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  1. Combined Tc-99m sesta MIBI scin

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    Enas Abdel Ghany Ibrahim

    2015-12-01

    Conclusions: Accurate localization of adenomas should be obtained prior to surgery for minimal invasive surgical procedure. In this study, combined ultrasonography and Tc-99m MIBI improved localization of parathyroid adenomas.

  2. Combined Tc-99m sesta MIBI scin

    OpenAIRE

    Enas Abdel Ghany Ibrahim; Momena Essam Elsadawy

    2015-01-01

    Purpose: To evaluate the role of combined high resolution ultrasonography and technetium Tc-99m methoxyisobutylisonitrile (MIBI) parathyroid scintigraphy in preoperative detection and localization of parathyroid adenoma, for better operative outcome. Patients and methods: 40 patients were included in this study, 25 of them were females and 15 were males, with age ranging between 31 and 75 (mean 46 ± 15). All patients presented with some of symptoms of hyperparathyroidism with venous blood ...

  3. Evaluation of myocardial perfusion using Tc-99m MIBI

    International Nuclear Information System (INIS)

    Tc-99m MIBI (methoxy isobutyl isonitrile) is newly introduced cationic myocardial perfusion agent. For its favorable dosimetry, upto 30 mCi of Tc-99m MIBI can be used in a patient. Therefore, unlike T1-201, first-pass ejection fraction, ventricular wall motion, and SPECT studies are readily evaluated using Tc-99m MIBI on patients who are undergoing myocardial perfusion study. Nineteen individuals entered into this study and 9 of 19 had T1-201 myocardial scintiscan. For the detection of myocardial ischemia, an IV dipyridamole with hand-grip or bicycle exercise was used. Tc-99m MIBI scintigraphic findings are compared to patients 'EKG' T1-201 scans and coronary angiograms. The result were summerized as follows; 1. In 9 cases, Tc-99m MIBI studies were comparable to T1-201 scintiscans. 2. In the remaining 10 cases, Tc-99m MIBI images were correlated well with clinical pictures, EKG, and angiographic findings. 3. Using Tc-99m MIBI, one can easily obtain fist-pass ejection fraction, ventricular wall motion and SPECT studies. It was concluded that Tc-99m MIBI seems to be an excellent agent for myocardial perfusion study. Tc-99m MIBI images are superior in quality than T1-201 images mainly due to high photon yield and ideal energy of Tc-99m MIBI

  4. Technique using Technetium-99m-MIBI Uptake Kinetics

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    Shinro Matsuo

    2013-04-01

    Full Text Available Mitochondria play an important role in energy production for the cell. The proper function of a myocardial cell largely depends on the functional capacity of the mitochondria.  Therefore it is necessary to establish a novel and reliable method for a non-invasive assessment of mitochondrial function and metabolism in humans. Although originally designed for evaluating myocardial perfusion, 99mTc-MIBI can be also used to evaluate cardiac mitochondrial function. In a clinical study on ischemic heart disease, reverse redistribution of 99mTc-MIBI was evident after direct percutaneous transluminal coronary angioplasty. The presence of increased washout of 99mTc-MIBI was associated with the infarct-related artery and preserved left ventricular function. In non-ischemic cardiomyopathy, an increased washout rate of 99mTc-MIBI, which correlated inversely with left ventricular ejection fraction, was observed in patients with congestive heart failure. Increased 99mTc-MIBI washout was also observed in mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS and in doxorubicin-induced cardiomyopathy. Noninvasive assessment of cardiac mitochondrial function could be greatly beneficial in monitoring possible cardiotoxic drug use and in the evaluation of cardiac damage in clinical medicine.

  5. Comparative 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin uptake in human soft tissue sarcoma cell lines

    International Nuclear Information System (INIS)

    The uptake characteristics of technetium-99m hexakis-2-methoxyisobutylisonitrile (MIBI), 99mTc-tetrofosmin and 99mTc-furifosmin in human soft tissue sarcoma cell lines were investigated and compared. After 10-120 min of incubation at 37 C, 32 C and 22 C with 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin, the kinetics of cellular uptake of these tracers in human soft tissue sarcoma cells SW 684 (fibrosarcoma), SW 872 (liposarcoma), SW 982 (synovial sarcoma) and SW 1353 (chondrosarcoma) was assessed. The uptake of 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin was temperature dependent. The kinetics of uptake of 99mTc-MIBI and of 99mTc-tetrofosmin was similar between fibrosarcoma and liposarcoma cells, as well as between synovial sarcoma and chondrosarcoma cells. 99mTc-furifosmin showed similar uptake kinetics in all cell lines. The uptake of 99mTc-furifosmin was, however, significantly higher in liposarcoma than in the other cells. The data indicate that the cellular uptake of 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin is dependent on cellular metabolic activity. (orig.)

  6. A Comparative Study of 99mTc-YIGSR and 99mTc-MIBI Uptake in Tumor Cells

    Institute of Scientific and Technical Information of China (English)

    HU Jia; LAN Xiaoli; ZHANG Yongxue; GAO Zairong; WU Jun

    2005-01-01

    To investigate a new kind of tumor tracer 99mTc-YIGSR developed from a five amino structure (YIGSR) of the Laminin -chain,which can bind to the laminin receptors of tumor specifically, and radiolabeled with MAG3. (1) Preparation of the 99m Tc-YIGSR probe: with S-Acetly-NH3-MAG3as the chelator and with proper reductants YIGSR was labeled with 99mTc; (2) Cell culture and viability measurement: EAC was maintained in RPMI 1640 supplemented with calf serum; the trypan blue exclusion was applied to calculate the cell viability; (3) Study of the cell dynamic: The EACs uptake of 99mTc-YIGSR and99mTc-MIBI was observed at 37 ℃ and 22 ℃, respectively. (1)The labeling efficiencies of 99mTc-YIGSR and99mTc-MIBI were (62±3) % and (96±2) %, respectively; (2) The cell viability was declined with time of incubation; (3) At 37 ℃, the EACS uptake of 99mTc-YIGSR and99mTc-MIBI reached the peak of (43. 16±2.4) % and (24.4±1.8) % at 60min, respectively; and at 22 ℃, the highest uptake was (26.5±2.1) % and (9. 47±1.9) % at 60min, respectively. The in vitro study suggests that 99mTc-YIGSR is superior to 99mTc-MIBI in cell uptake and has potential value in tumor imaging.

  7. Pituitary Incidentalomas Detected with Technetium-99m MIBI in Patients with Suspected Parathyroid Adenoma: Preliminary Results

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    Tiktinsky, Ekaterina; Horne, Tifha; Friger, Michael; Agranovich, Svetlana; Lantsberg, Sophie

    2012-01-01

    Tc-99m MIBI (MIBI) is a cationic lipophilic agent, which has traditionally been used for myocardial perfusion scintigraphy, detection and monitoring of different benign and malignant tumors. The objective of this study was to evaluate the frequency of pituitary incidentalomas detected on MIBI scans performed on patients with suspected parathyroid adenomas and to provide semiquantitative analysis of tracer uptake in the pituitary region. Tomographic images of MIBI scans on 56 patients with sus...

  8. Different dynamic of Tc-99m-tetrofosmin and Tc-99m-MIBI in a parathyroid adenoma; Unterschiedliche Dynamik von Tc-99m-Tetrofosmin und Tc-99m-MIBI in einem Nebenschilddruesen-Adenom

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    Nowak, B.; Sabri, O.; Reinartz, P.; Kleinhans, E.; Zimny, M. [Universitaetsklinikum Aachen (Germany). Klinik fuer Nuklearmedizin; Hoff, C. [Universitaetsklinikum Aachen (Germany). Medizinische Klinik III

    1999-07-01

    Case report of a patient with hypercalcemia and suspected parathyroid adenoma. Because of a previous strumectomy a precise preoperative localization of the suspected parathyroid adenoma was demanded. Tc-99m-tetrofosmin scintigraphy in double phase technique failed to detect a parathyroid adenoma by failing to show a region of increased focal uptake with delayed washout in relation to the thyroid gland. Only comparison of the Tc-99m-tetrofosmin images with a Tc-99m-pertechnetate scan revealed a right caudal parathyroid adenoma. A double phase Tc-99m-MIBI study of the same patient was able to localize this parathyroid adenoma without the need of a corresponding Tc-99m-pertechnetate scintigraphy due to a differential washout with persistent focal uptake in the parathyroid adenoma and a progressively decreasing uptake in the thyroid tissue. This case indicates that Tc-99m-tetrofosmin is a suitable agent for parathyroid imaging only if used together with Tc-99m-pertechnetate but is seems to lack the differential washout characteristics of Tc-99m-MIBI according parathyroid gland and thyroid gland. (orig.) [German] Bei einer Patientin mit Hyperkalzaemie und Verdacht auf primaeren Hyperparathyreoidismus wurde wegen einer vorherigen Strumektomie eine genaue praeopertive Lokalisationsdiagnostik gefordert. Eine in Zwei-Phasen-Technik durchgefuehrte Szintigraphie mit Tc-99m-Tetrofosmin allein erbrachte keinen sicheren Nachweis eines Nebenschilddruesen-Adenoms, da an keiner Lokalisation eine fokale Mehranreicherung mit relativ zur Schilddruese verzoegertem Washout festzustellen war. Erst der Vergleich mit dem Tc-99m-Pertechnetat-Szintigramm ergab den Hinweis auf ein Nebenschilddruesen-Adenom rechts kaudal. Eine zusaetzlich durchgefuehrte Tc-99m-MIBI-Szintigraphie konnte dieses Adenom alleine aufgrund einer hier vorhandenen Persistenz der Nuklidspeicherung im Nebenschilddruesen-Adenom bei gleichzeitig deutlichem Washout aus der uebrigen Schilddruese nachweisen. Dies weist darauf

  9. Localization of primary hyperparathyroidism using {sup 99m}Tc-MIBI scintigraphy

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    Yane, Katsunari; Emoto, Mie; Okamoto, Hideyuki; Yuki, Kazue; Hosoi, Hiroshi [Nara Medical Univ., Kashihara (Japan); Miyahara, Hiroshi [Osaka Prefectural Hospital (Japan)

    2003-03-01

    To determine the clinical utility of 99mTc-MIBI scintigraphy (MIBI) in the localization of hyperfunctioning parathyroid lesions, detection of lesions in 14 patients with primary hyperparathyroidism was compared between MIBI, ultrasonography and computed tomography (CT). MIBI (85.7%) and ultrasonography (78.5%) demonstrated higher detection rates than CT (50%). Furthermore, combination of MIBI and ultrasonography (92.8%) increased the detection rate. One case of ectopic parathyroid adenoma was detected only on MIBI. The smallest parathyroid lesion detected using MIBI was a parathyroid adenoma weighing 300 mg. A combination of MIBI and ultrasonography is therefore useful for the localization of primary hyperparathyroidism, and MIBI is particularly useful for detecting ectopic lesions. (author)

  10. The usefulness of 99mTc-MIBI in the detection of active pulmonary tuberculosis

    International Nuclear Information System (INIS)

    The use of radiopharmaceuticals in evaluation of pulmonary tuberculosis may help to resolve difficult diagnostic problems such as discordance between sputum examinations and chest roentgenographic findings. We investigated the usefulness of 99mTc-methoxyisobutylisonitrile (MIBI) scintigraphy in the detection of active pulmonary tuberculosis. Forty-six patients with suspected active pulmonary tuberculosis were studied with sputum smear of AFB, sputum AFB culture, chest X-ray and MIBI scan. MIBI image was obtained 15 and 60 min after intravenous injection of 370MBq(10mCi) 99mTc-MIBI. In 16 patients of them Ga scans were performed in addition to MIBI scan. Repeated MIBI scans were done in 7 patients with active pulmonary tuberculosis after 4∼6 months of antituberculous chemotherapy. Thirty-two patients were confirmed as active tuberculosis by sputum culture. Sensitivity of MIBI scan to active tuberculosis was 87.5%(28/32) and MIBI findings were negative in all of 14 patients with inactive disease. Focal uptake of MIBI was dense in the area that was strongly suggested active tuberculous lesions by chest roentgenogram. There was no discordance between MIBI and Ga image in 16 patients. But the uptake areas of Ga images were broader than that of MIBI images. After 4∼6 months of antituberculous treatment all repeated MIBI scans revealed negative findings except 1 patient with persistent active pulmonary tuberculosis due to drug resistance. MIBI scan could be used in the detection of active pulmonary tuberculosis as a useful noninvasive diagnostic tool

  11. Different dynamic of Tc-99m-tetrofosmin and Tc-99m-MIBI in a parathyroid adenoma

    International Nuclear Information System (INIS)

    Case report of a patient with hypercalcemia and suspected parathyroid adenoma. Because of a previous strumectomy a precise preoperative localization of the suspected parathyroid adenoma was demanded. Tc-99m-tetrofosmin scintigraphy in double phase technique failed to detect a parathyroid adenoma by failing to show a region of increased focal uptake with delayed washout in relation to the thyroid gland. Only comparison of the Tc-99m-tetrofosmin images with a Tc-99m-pertechnetate scan revealed a right caudal parathyroid adenoma. A double phase Tc-99m-MIBI study of the same patient was able to localize this parathyroid adenoma without the need of a corresponding Tc-99m-pertechnetate scintigraphy due to a differential washout with persistent focal uptake in the parathyroid adenoma and a progressively decreasing uptake in the thyroid tissue. This case indicates that Tc-99m-tetrofosmin is a suitable agent for parathyroid imaging only if used together with Tc-99m-pertechnetate but is seems to lack the differential washout characteristics of Tc-99m-MIBI according parathyroid gland and thyroid gland. (orig.)

  12. The Reevaluation of MIBI as the Ligand of 99mTc-MIBI Radiopharmaceutical for Myocardial Imaging

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    Z. Nurlaila

    2008-01-01

    Full Text Available Methoxy isobutyl isonitrile labelled with technetium-99m (99mTc radionuclide can be used for the evaluation of acute myocardial infarction. The 99mTc-MIBI radiopharmaceutical is available in the lyophilized-kit, which is separately packed with its radionuclide. Recently, in CNTMR-BATAN Bandung, 99mTc-MIBI radiopharmaceutical was prepared using MIBI ligand synthesized in 1996 as lyophilized-kits having the radiochemical purity less than 90 % and not accumulated in the myocardium. It could be caused by several factors, among others were technical preparation of lyophilized dry-kit and the quality of MIBI; therefore the reevaluation of the ligand for the quality improvement was needed. Firstly, MIBI ligand was recristallized. It was found that MIBI as [Cu(MIBI4]BF4 salt was a glassy solid with the melting point of 98 -99 oC. The infra red analysis showed the presence of spectra vibration at 1085, 1180 cm-1 and 2180, 2220 cm-1 for –OCH3 and N≡C groups, respectively. The preparation of 99mTc-MIBI was performed by adding 99mTc radionuclide into new MIBI liquid-kit, then boiled in water bath for minimum period of 10 minutes. The radiochemical purity of 99mTc-MIBI was analysed by thin layer chromatographic method using absolute ethanol as a mobile phase. The pre-clinical evaluation was observed in Wistar rat and clinical studies were performed in volunter using gamma camera. It is revealed that the radiochemical purity of more than 90 % i.e. 96.83 ± 1.43 % was still stable until 24 hours storage at room temperature. The stability studies of 99mTc-MIBI in human blood plasma showed that in 6 hours, the radiochemical purity was decreased to 90.89 ± 2.90 %. Clinical application of 99mTc-MIBI with tomography techniques using gamma camera showed the distinct accumulation of radioactivity in the heart.

  13. A correlative study between {sup 99m}Tc-ESTCPTA and {sup 99m}Tc-MIBI in rats

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    Unak, Perihan E-mail: unak@bornova.ege.edu.tr; Enginar, Huseyin; Zuemruet Biber, F.; Yurt Lambrecht, Fatma; Aslani, M.A.A.; Ozkilic, Hayal

    2002-11-01

    Tissue distribution of the {sup 99m}Tc labeled derivative of the estrogen compound 3,17-{alpha}-estradiolyl propyl 1,4,8,11-tetraazacyclotetradecanyl-1-(4-methylbenzoic acid) ester (ESTCPTA), which has an 3,17-{alpha}-estradiolyl propinol coupled to 1-(4-methylbenzoic acid)1,4,8,11-tetraazacyclotetradecane (CPTA), was compared to {sup 99m}Tc-MIBI (methoxyisobutyl isonitrile) in female Albino Wistar rats. Tissues of interest included lung, liver, heart, kidneys, spleen, stomach, intestines, pancreas, muscle, blood, breast, ovary, fat, and uterus. {sup 99m}Tc-ESTCPTA uptake by the uterus and ovary, as ER-rich tissues, was highly selective. Maximum uptakes for {sup 99m}Tc-MIBI and {sup 99m}Tc-ESTCPTA are 90 min in breast, ovary and uterus. The pancreas also showed significant receptor saturated and unsaturated ratios for {sup 99m}Tc-ESTCPTA. Results are sufficiently encouraging to generate further evaluation of these and related compounds as possible estrogen receptor based tumor imaging and therapeutic agents in estrogen-rich tissues.

  14. [Complementary scintigraphic examinations (99mTc-MDP, 99mTc-MIBI and Octreoscan) in breast neoplasm assessment].

    Science.gov (United States)

    Stefănescu, C; Chossiere, L; Rusu, V; Meignan, M

    1996-01-01

    Scintigraphic imagery was used in breast carcinoma mostly for the bone metastases screening, using 99mTc-MDP. Others radiopharmaceuticals can be used to visualize either the primary tumour or the soft tissues metastases. It's mainly the case of 99mTc-MIBI, and also of some somatostatin analogues, like Octreoscan. This case report of a patient with a T3N1M+ breast adenocarcinoma having different secondary sites represent also a prove of the complementarity of these explorations, which give the possibility of diagnostic and therapeutic evaluation and, in the same time, the possibility of a better understood of the metastasizing mechanism. PMID:9455458

  15. Bone marrow uptake of 99mTc-MIBI in patients with multiple myeloma

    International Nuclear Information System (INIS)

    In a previous study, we showed the ability of technetium-99m methoxyisobutylisonitrile (99mTc-MIBI) scan to identify active disease in patients with multiple myeloma (Eur J Nucl Med 1998; 25: 714-720). In particular, a semiquantitative score of the extension and intensity of bone marrow uptake was derived and correlated with both the clinical status of the disease and plasma cell bone marrow infiltration. In order to estimate quantitatively 99mTc-MIBI bone marrow uptake and to verify the intracellular localization of the tracer, bone marrow samples obtained from 24 multiple myeloma patients, three patients with monoclonal gammopathy of undetermined significance (MGUS) and two healthy donors were studied for in vitro uptake. After centrifugation over Ficoll-Hypaque gradient, cell suspensions were incubated with 99mTc-MIBI and the uptake was expressed as the percentage of radioactivity specifically retained within the cells. The cellular localization of the tracer was assessed by micro-autoradiography. Twenty-two out of 27 patients underwent 99mTc-MIBI scan within a week of bone marrow sampling. Whole-body images were obtained 10 min after intravenous injection of 555 MBq of the tracer; the extension and intensity of 99mTc-MIBI uptake were graded using the semiquantitative score. A statistically significant correlation was found between in vitro uptake of 99mTc-MIBI and both plasma cell infiltration (Pearson's coefficient of correlation r=0.69, P99mTc-MIBI inside the plasma cells infiltrating the bone marrow. Therefore, our findings show that the degree of tracer uptake both in vitro and in vivo is related to the percentage of infiltrating plasma cells which accumulate the tracer in their inner compartments. (orig.)

  16. Preliminary clinical observation of 99mTc-MIBI breast tumor imaging

    International Nuclear Information System (INIS)

    An effective, noninvasive diagnostic method of breast cancer is investigated. 99mTc-MIBI breast tumor imaging was performed in 78 patients with palpable breast mass. All was pathologically proved after operation. Of 78 patients, 42 were breast carcinoma, among them 35 were detected using 99MTc-MIBI scintigraphy. The smallest detectable mass was a infiltrating ductal carcinoma measuring 1.5 cm x 1.5 cm x 1.2 cm. Of 36 patients with benign lesions, 30 with negative result, among the 6 positive one, 5 were big adenoma, 1 was plasma cell mastitis. The sensitivity and specificity of 99mTc-MIBI imaging in detecting breast cancer wa 83.3% either. 99mTc-MIBI scintigraphy can be used as an accessory method in detecting breast cancer. But it was useless for differentiation between breast cancer and big adenoma

  17. Incidentally detected diaphragmatic hernia mimicking angina pectoris with Tc-99m MIBI myocardial perfusion imaging

    Science.gov (United States)

    Cınar, Alev; Sadıc, Murat; Demırel, Koray; Korkmaz, Meliha

    2013-01-01

    In recent years 99mTechnetium methoxy-isobutyl-isonitrile (99mTc MIBI) is widely used in the evaluation of myocardial perfusion imaging (MPI). In this imaging protocol besides the cardiac evaluation, numerous other organs are included in the field of view. 99mTechnetium MIBI is taken up in all metabolically active tissue in the body except for the brain. Extracardiac uptake patterns as benign or serious conditions can be revealed during the MPI. In the imaging protocol, we should be aware of distribution of this radiotracer in order to recognize the abnormal uptake. Here in, we present a large diaphragmatic hernia, seemed as a mirror vision of myocardium that was determined incidentally during the MPI with 99mTc MIBI. PMID:24379540

  18. The value of {sup 99m}technetium-MIBI scintiscanning for diagnostic evaluation of breast cancer; Stellenwert der {sup 99m}Technetium-MIBI-Szintigraphie in der Mammadiagnostik

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    Hagedorn, K.; Kraemer, S.; Lang, N. [Universitaets-Frauenklinik, Erlangen (Germany); Schulz-Wendtland, R.; Bautz, W. [Universitaet Erlangen-Nuernberg (Germany) Institut fuer Diagnostische Radiologie; Kat, S.; Wolf, F. [Universitaet Erlangen-Nuernberg (Germany). Nuklearmedizinische Klinik

    1998-03-01

    We performed {sup 99m}Tc-MIBI scintiscanning in 42 patients with indications for surgery of mammary lesions, ascertained by palpation, mammography, ultrasonography, or MRI. We had a total of 62 histologically examined tumors for our analysis, comprising 24 benign tumors (10 fibroadenomas, 1 papilloma, 1 cyst, 1 lymph node, 10 mastopathies), 4 in-situ carcinomas, and 34 malignant tumors (32 duct carcinomas, 1 intramammary lymph node matastasis, 1 low-grade malignant sarcoma). The benign mammary gland tumors were not shown by contrast agent accumulation in the {sup 99m}Tc-MIBI scintiscans. Out of 14 carcinomas of a size less than 15 mm across, only 5 were detected by scintiscanning, and none of the in-situ carcinomas was revealed in the scintiscans. However, all malignomas of a size of 15 mm or more were detected by scintiscanning. The results of the comparative analysis of the diagnostic methods relating to sensitivity and specificity are (n=58, without CIS):{sup 9}9mTc-MIBI scintiscans: 73.5%/100%; mammography: 94,1%/62.5%; palpation:82.4%/91.7%; ultrasonography:94.1%/58.3%. As compared to the other four methods, {sup 9}9mTc-MIBI scintiscanning ranks last in terms of sensitivity, and is the best method interms of specificity. The high specificity shown by our study could not be confirmed by other studies, and the sensitivity is particularly restricted when tumors are small in size, or at unfavourable locations. The conclusion therefore is that this scintiscanning method is not to be recommended as a screening method, nor is it a method of choice for complementary diagnostic evaluation of breast lesions, except perhaps for a few specific cases. (orig./CB) [Deutsch] Wir fuehrten die {sup 99m}Tc-MIBI-Szintigraphie bei 42 Patientinnen mit operationsbeduerftigem Mammabefund durch, die zuvor palpatorisch, mammographisch, sonographisch und zum Teil mittels Magnetresonanztomographie der Mamma untersucht worden waren. Insgesamt konnten wir 62 histologisch untersuchte

  19. Comparison of uptake of 99mTc-MIBI, 99mTc-tetrofosmin and 99mT-Q12 into human breast cancer cell lines

    International Nuclear Information System (INIS)

    Technetium-99m hexakis-2-methoxyisobutyl-isonitrile (MIBI), 99mTc-tetrofosmin and 99mTc-Q12 were all introduced for myocardial imaging but found additional applications as they are taken up by different tumours, enabling imaging of these lesions in patients. The aim of this study was to compare the uptake characteristics of these compounds in vitro in the human adenocarcinoma breast cell lines MCF-7 and ZR-75. It was shown that 99mTc-MIBI had the highest cellular uptake (15.9%±0.5% dose/mg protein after 60 min in MCF-7, and 14.2%±0.4% dose/mg protein in ZR-75), followed by 99mTc-tetrofosmin (6.8%±0.6% dose/mg protein in MCF-7, and 8.2%±0.2% dose/mg protein in ZR-75) and 99mTC-Q12 (3.2%±0.1% dose/mg protein in MCF-7, and 3.5%±0.3% dose/mg protein in ZR-75 cells). For all three compounds tenfold differences in specific activity did not influence total cell-associated radioactivity. Uptake of 99mTc-MIBI and 99mTc-tetrofosmin was obviously lower at 4 C than at 37 C, whereas 99mTc-Q12 uptake showed only slight temperature dependence. When uptake was compared in cells grown to different cell densities (1 mg/ml cellular protein versus 0.3 mg/ml), no differences in uptake were detected when uptake was corrected for the amount of cellular protein present in the dishes. Furthermore, for all compounds it was shown that cellular radioactivity decreased rapidly after washing. Apart from the differences in cellular uptake of the three compounds after 60 min, no differences in residual cellular radioactivity after washing were found between the different compounds when expressed as a percentage of their 60-min uptake, suggesting that the efflux process of the radiolabelled compounds was similar. The differences in cell-associated activity after 60 min were thus presumably caused by differences in uptake. (orig./MG)

  20. Evaluation of multiple myeloma with technetium-99m-MIBI scintigraphy

    International Nuclear Information System (INIS)

    Aim: To evaluate the clinical utility of whole-body scintigraphy with 99mTc-MIBI in the detection of bone marrow involvement in patients (pts) with multiple myeloma (MM) and related monoclonal disease, in assessing a disease extent and activity, and to define the relationship of different patterns of 99mTc-MIBI uptake with clinical status and stage of disease. Material and Methods: Eighty-three consecutive pts (40 men and 43 women) with MM or monoclonal gammopathy of undetermined significance (MGUS) enrolled in this study were divided into three subgroups: 1. Forty-three MM pts with active disease (AD) in stage II or III according to standard criteria, 2. Thirteen pts in complete remission (CR), and 3. Twenty-seven MGUS pts or MM pts in stage IA. Whole-body scans were obtained 10 min after the i.v. injection of 740 MBq 99mTc-MIBI in anterior and posterior view. The scans were scored semi-quantitatively according to extension and intensity of tracer uptake. Results: Thirty-nine MM pts in the first subgroup with AD showed different patterns of 99mTc-MIBI uptake of high intensity while 4 exhibited physiological distribution of tracer. All thirteen pts in CR in the second subgroup showed a negative 99mTc-MIBI scan. In the third subgroup, twenty-three MGUS pts had no pathological uptake of the radiotracer while 3 MM pts in stage IA presented the diffuse and one both focal and diffuse pattern of 99mTc-MIBI uptake. The uptake score correlated significantly with clinical status and stage of disease and its activity as determined by infiltration of bone marrow by plasma cells (p<0.05), paraprotein (p<0.05), beta2-microglobulin (p<0.05), serum thymidinekinase (p<0.05) and telopeptide-ICTP (p<0.05). Overall sensitivity of the scintigraphic procedure was 91% and specificity 90%. Conclusion: Our results show that whole-body 99mTc-MIBI scintigraphy provides important information which is clinically useful in the detection of bone marrow involvement and in assessing the extent

  1. Iterative reconstruction: an improvement of technetium-99m MIBI SPET for the detection of parathyroid adenomas?

    Energy Technology Data Exchange (ETDEWEB)

    Moka, D.; Eschner, W.; Voth, E.; Dietlein, M.; Schicha, H. [Dept. of Nuclear Medicine, University of Cologne (Germany); Larena-Avellaneda, A. [Dept. of Surgery, St. Katharinen-Hospital, Frechen (Germany)

    2000-05-01

    The purpose of this study was to assess the value of technetium-99m methoxyisobutylisonitrile (MIBI) single-photon emission tomography (SPET) and an iterative reconstruction algorithm for the preoperative localisation of parathyroid adenomas (PTAs). Seventy-two patients (26 male, 46 female, mean age 58{+-}16 years) with known primary hyperparathyroidism were examined preoperatively. First, a thyroid examination was performed to detect possible MIBI-accumulating thyroid lesions. Planar scans were then acquired 15 and 120 min and tomographic images 120 min after intravenous injection of 740 MBq {sup 99m}Tc-MIBI, using a triple-head gamma camera (Picker Prism 3000). Additionally, {sup 99m}Tc-MIBI/ {sup 99m}Tc-pertechnetate subtraction scintigraphy of the early planar images was performed. The SPET data were evaluated using an iterative reconstruction (multiplicative iterative SPET reconstruction: MISR) as well as a standard algorithm (FBP: filtered back-projection with application of a 3-D low-pass postfilter). The weight of the resected PTAs ranged from 110 mg to 5 g. Using planar MIBI scans, correct localisation of the side of the PTA was possible in 81% of cases (58% for PTAs weighing less than 500 mg). Sensitivity increased to 94% using SPET and FBP, while with MISR it rose further, to 97%. Patients with PTAs weighing less than 500 mg showed a sensitivity of 88% with MISR and 81% with FBP. Furthermore, there was a clear improvement in image quality using MISR. None of the normal parathyroid glands were visualised. This study indicates that, in comparison with planar scintigraphy, {sup 99m}Tc-MIBI SPET is a more sensitive and specific tool for topographical localisation of PTAs, especially those that are small. There is a further improvement in sensitivity and image quality when iterative reconstruction is used instead of FBP. (orig.)

  2. Bone marrow uptake of {sup 99m}Tc-MIBI in patients with multiple myeloma

    Energy Technology Data Exchange (ETDEWEB)

    Fonti, R. [Medicina Nucleare, Facolta di Medicina, Univ. Federico II, Naples (Italy); Centro per la Medicina Nucleare C.N.R., Cattedra di Medicina Nucleare, Napoli (Italy); Del Vecchio, S.; Zannetti, A.; Di Gennaro, F.; Pace, L.; Salvatore, M. [Centro per la Medicina Nucleare C.N.R., Cattedra di Medicina Nucleare, Napoli (Italy); De Renzo, A.; Catalano, L.; Califano, C.; Rotoli, B. [Cattedra di Ematologia, Dipt. di Medicina Clinica e Sperimentale, Napoli (Italy)

    2001-02-01

    In a previous study, we showed the ability of technetium-99m methoxyisobutylisonitrile ({sup 99m}Tc-MIBI) scan to identify active disease in patients with multiple myeloma (Eur J Nucl Med 1998; 25: 714-720). In particular, a semiquantitative score of the extension and intensity of bone marrow uptake was derived and correlated with both the clinical status of the disease and plasma cell bone marrow infiltration. In order to estimate quantitatively {sup 99m}Tc-MIBI bone marrow uptake and to verify the intracellular localization of the tracer, bone marrow samples obtained from 24 multiple myeloma patients, three patients with monoclonal gammopathy of undetermined significance (MGUS) and two healthy donors were studied for in vitro uptake. After centrifugation over Ficoll-Hypaque gradient, cell suspensions were incubated with {sup 99m}Tc-MIBI and the uptake was expressed as the percentage of radioactivity specifically retained within the cells. The cellular localization of the tracer was assessed by micro-autoradiography. Twenty-two out of 27 patients underwent {sup 99m}Tc-MIBI scan within a week of bone marrow sampling. Whole-body images were obtained 10 min after intravenous injection of 555 MBq of the tracer; the extension and intensity of {sup 99m}Tc-MIBI uptake were graded using the semiquantitative score. A statistically significant correlation was found between in vitro uptake of {sup 99m}Tc-MIBI and both plasma cell infiltration (Pearson's coefficient of correlation r=0.69, P<0.0001) and in vivo score (Spearman rank correlation coefficient r=0.60, P<0.01). No specific tracer uptake was found in bone marrow samples obtained from the two healthy donors. Micro-autoradiography showed localization of {sup 99m}Tc-MIBI inside the plasma cells infiltrating the bone marrow. Therefore, our findings show that the degree of tracer uptake both in vitro and in vivo is related to the percentage of infiltrating plasma cells which accumulate the tracer in their inner

  3. Myeloma, source of false positive of parathyroid scintigraphy with {sup 99m}Tc-MIBI; Le myelome, source de faux-positif de la scintigraphie parathyroidienne au 99mTc-MIBI

    Energy Technology Data Exchange (ETDEWEB)

    Zaabar, L.; Ben Sellem, D.; El Ajmi, W.; Slim, I.; Letaief, B.; Mhiri, A.; Ben Slimene, M.F. [Institut Salah Azaiez, Service de medecine nucleaire, Tunis (Tunisia)

    2010-07-01

    Purpose: Considering a specificity of the MIBI dual-phase parathyroid scintigraphy with {sup 99m}Tc-MIBI presents false positives which number has declined since the systematic implementation of the SPECT. We report a case of a superior mediastinal false-positive. Conclusions: the myeloma represents a potential cause of false-positive of parathyroid scintigraphy with {sup 99m}Tc-MIBI. (N.C.)

  4. Lesion localization in patients with hyperparathyroidism using double-phase Tc-99m MIBI parathyroid scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Jung Woo; Ryu, Jin Sook; Kim, Jae Seung; Moon, Dae Hyuk; Hong, Seung Mo; Gong, Gyung Yub; Hong, Suk Joon; Lee, Hee Kyung [College of Medicine, Ulsan Univ., Seoul (Korea, Republic of)

    1999-02-01

    This study was performed to evaluate the diagnostic usefulness of double-phase Tc-99m MIBI parathyroid scintigraphy with single photon emission computed tomography (SPECT) in patients with hyperparathyroidism. We also evaluated the relationship between Tc-99m MIBI uptake and oxyphil cell contents in parathyroid glands. The subjects were 28 parathyroid glands of 10 patients who underwent Tc-99m MIBI parathyroid scintigraphy and parathyroidectomy for clinically suspected hyperparathyroidism. Early and delayed pinhole images were obtained at 15 minutes and 2 hours after injection of Tc-99m MIBI, and SPECT images were followed. The weight and oxyphil cell contents of parathyroid tissue were obtained from pathologic specimen, and the scintigraphic findings were compared with histopathology. In surgical histopathology, 6 parathyroid adenomas and 9 parathyroid hyperplasias were confirmed. The sensitivity, specificity, and positive predictive value of early and delayed images were 46.7% (7/15), 76.9% (10/13), 70% (7/10) and 66.7% (10/15), 92.3% (12/13), 90.9% (10/11), respectively. SPECT image detected an additional small hyperplasia. The sensitivity, specificity, and positive predictive value of combined interpretation of early and delayed images with SPECT were 73.3% (11/15), 100% (13/13), 100% (11/11). The sensitivity was 100% (6/6) for adenoma, whereas that was 55.5% (5/9) for hyperplasia. Both adenomas and hyperplasias showed significantly increased oxyphil cell contents compared with normal parathyroid glands (p<0.0001), but the oxyphil cell content and weight were not significantly different between adenomas and hyperplasias. Double-phase Tc-99m MIBI parathyroid scintigraphy with SPECT is useful for lesion localization in patients with hyperparathyroidism. Although both adenoma and hyperplasia have increased oxyphil cell content, the sensitivity is high in adenoma, but low in hyperplasia.

  5. Mammography and 99mTc-MIBI scintimammography in breast cancer diagnosis

    International Nuclear Information System (INIS)

    The aim of this work has been to evaluate whether a diagnostic protocol based on the joint use of mammography and 99mTc-MIBI scintimammography can help to distinguish the lesions and to reduce the number of biopsies required in patients with suspected breast cancer. A total of 58 women were evaluated by palpation, mammography, scintimammography. Twenty-four patients were scintimammographed with 99mTc-MIBI at 10 min after injection. Thirty-four patients were taken double phase scintimammography with 99mTc-MIBI 10 min and 60-90 min after injection. Based on mammography, the suspicion degrees of malignancy were rated, and 30 results of malignancy were confirmed by histopathology. Based on mammography, 18 lesions were considered to be most probably benign (of which 3 were histopathologically breast cancer), 19 as indeterminate (of which 9 were histopathologically breast cancer), and 21 as malignant (of which 18 were histopathologically breast cancer). The results of early and delayed phases 99mTc-MIBI scintimammography were the same. The sensitivity, specificity and accuracy of scintimammography were 74.29%, 86.96% and 79.31%, respectively. Scintimammography gave 16 correct diagnosis in 19 mammogram indeterminate (84.2%) and demonstrated 5 out of 8 cases axillary lymph nodes metastasis (62.5%). These studies show that 99mTc-MIBI scintimammography used as a complementary testing technique to mammography is useful in the examination of patients with suspected breast cancer. The adoption of a joint mammography-scintimammography diagnostic protocol could considerably reduce the number of biopsies performed in patients with lesions of indeterminate mammographic suspicion of malignancy

  6. Tc-99m-MIBI brain SPECT in differentiating tumor recurrences from necrosis

    International Nuclear Information System (INIS)

    Brain SPECT using 99m-TC MIBI can distinguish between local tumor recurrence and radio necrosis of the primary brain tumor, whereas CT scan and MRI do not have this ability. 1. Is it possible to search for tumoral cells in the brain by using TC-99m MIBI? 2. How sensitive and specific is the SPECT in distinguishing the presence of active tumor in the brain and differentiating it from post-therapy necrosis? 3. Is it feasible to substitute this diagnostic modality for stereotactic biopsy surgery? Patients who presented to the neurosurgery clinic with the clinical manifestations of brain tumor relapse between 22nd August 1999 and 1.; February 2000 and were candidates for stereotactic biopsy were chosen. A 99m-TC MIBI SPECT was performed before biopsy. The total number of patients was 13. Five patients had the diagnosis of brain tumor by surgery and biopsy and had undergone a course of radiotherapy and chemotherapy. These patients were normal clinically and MIBI SPECT was done for the purpose of follow-up. Clinical manifestations consisted of, Weakness, Vertigo visual disorders, loss of consciousness, headache, aphasia and hemiparesis. The primary tumors were composed of a variety of lei sons including: grade I, II astrocytoma (62.5%), glioblastoma (25%) and medulloblastoma (12.5%). eight patients who had MIBI SPECT firstly and then had biopsy, brain tumor relapse was reported by both biopsy and SPECT in seven patients. This proved a 100% sensitivity and a 100% specificity for MIBI SPECT in differentiating, between tumor relapse and necrosis, a result comparable to stereotactic biopsy. Also in 5 patients with clinical evidence of remission, MIBI SPECT was negative for tumor recurrence in all. Patients who present with the clinical manifestations of brain tumor relapse, usually have a history of surgery, radiotherapy or chemotherapy and any invasive procedures like stereotactic biopsy on these patients carries a high risk for anesthesia and surgery, besides being costly

  7. Technetium-99m tetrofosmin for parathyroid scintigraphy: a direct comparison with {sup 99m}Tc-MIBI, {sup 201}Tl, MRI and US

    Energy Technology Data Exchange (ETDEWEB)

    Wakamatsu, Hideyuki [Dept. of Radiology, Noguchi Thyroid Clinic and Hospital Foundation, Oita (Japan); Noguchi, Shiro; Yamashita, Hiroyuki [Dept. of Surgery, Noguchi Thyroid Clinic and Hospital Foundation, Oita (Japan); Yamashita, Hiroto [Dept. of Pathology, Noguchi Thyroid Clinic and Hospital Foundation, Oita (Japan); Tamura, Shozo; Jinnouchi, Seishi; Nagamachi, Shigeki; Futami, Shigemi [Dept. of Radiology, Miyazaki Medical College, Miyazaki (Japan)

    2001-12-01

    The aim of this study was to evaluate the efficacy and role of technetium-99m tetrofosmin for the detection of abnormal parathyroid glands to be referred for surgical treatment. Twenty-eight consecutive patients, including 25 primary and 3 secondary cases of hyperparathyroidism, were evaluated. {sup 99m}Tc-tetrofosmin/{sup 99m}Tc-pertechnetate subtraction scintigraphy (TF/Tc) was performed on all patients, and the results were directly compared with those of {sup 99m}Tc-methoxyisobutylisonitrile (MIBI)/{sup 99m}Tc-pertechnetate subtraction scintigraphy (MIBI/Tc), {sup 201}Tl/{sup 99m}Tc-pertechnetate subtraction scintigraphy (Tl/Tc), magnetic resonance imaging (MRI) and ultrasonography (US). In cases of single-gland disease, the sensitivities of TF/Tc, MIBI/Tc, Tl/Tc, MRI and US were 63.2%, 68.4%, 57.9%, 55.6% and 63.2%, respectively. In cases of multi-gland disease, the sensitivities of TF/Tc, MIBI/Tc, Tl/Tc, MRI and US were 41.7%, 41.7%, 37.5%, 58.3% and 54.2%, respectively. In cases of parathyroid adenoma, the sensitivities of TF/Tc, MIBI/Tc, Tl/Tc, MRI and US were 68.8%, 75.0%, 68.8%, 62.5% and 75.0%, respectively. In cases of parathyroid hyperplasia, the sensitivities of TF/Tc, MIBI/Tc, Tl/Tc, MRI and US were 40.7%, 40.7%, 33.3%, 53.8% and 48.1%, respectively. It is concluded that, for the detection of abnormal parathyroid glands, {sup 99m}Tc-tetrofosmin is as useful as {sup 99m}Tc-MIBI and is more useful than {sup 201}Tl. (orig.)

  8. Comparison of Tc-99m pertechnetate images with dual-phase Tc 99m MIBI and SPECT images in primary hyperparathyroidism

    Directory of Open Access Journals (Sweden)

    Sait Sager

    2014-01-01

    Full Text Available Background: The purpose of this study was to evaluate the value of Tc-99m pertechnetate planar, dual-phase MIBI and MIBI-SPECT images in the determination and localization of parathyroid lesions. Materials and Methods: In this study, 38 patients who underwent operation for primary hyperparathyroidism were included. Tc-99m pertechnetate planar-pinhole imaging of the neck and then MIBI planar and SPECT images in supine position was performed. Late SPECT images were acquired 120 minutes after the injection. Early and late MIBI images were quantitatively evaluated. Results: Of the 38 patients, 30 of them had adenoma, 2 patients had hyperplasia and 6 of them were normal on planar images. Thirty-four of 38 patients were positive on SPECT images. SPECT images of the patients with positive results were matched with pathology results. Conclusion: As a result, Tc-99m pertechnetate planar-pinhole, Tc-99m MIBI planar and SPECT images are useful for localization of parathyroid lesions especially in multinodular thyroid gland. However, US or CT images are necessary for more accurate localization and Tc-99m pertechnetate images are useful for interpreting and comparing with the early and late MIBI images.

  9. Synthesis of Cu[MIBI]4Cl to prepare 99m Tc [MIBI]6+ as a myocardial perfusion agent

    International Nuclear Information System (INIS)

    2-Methoxyisobutylisonitrile (MIBI) is a key starting material in the preparation of technetium-99m hexakis (2-methoxyisobutylisonitrile). This cationic complex has been reported to be clinically useful as myocardial perfusion agent. In this work, we designed an improved synthetic method to prepare 2-methoxyisobutylisonitrile in a two step synthesis with an overall yield of 52.9 % , on the basis of 2-methoxyisobutylamine is a primary amine which, when is heated with a mixture of chloroform and alcoholic potash, produces an isonitrile. Its spectroscopic properties as well as the preparation of its copper and technetium-99m complexes are also presented. (Author)

  10. Quantitative interpretation method for detection of head and neck cancer with Tc-99m MIBI SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Seok, Ju Won; Kim, In Ju; Kim, Yong Ki; Wang, Soo Geun [College of Medicine, Pusan National Univ., Pusan (Korea, Republic of)

    2001-07-01

    The aim of this study was to present a method for diagnosis of head and neck cancer with Tc-99m MIBI SPECT by creating regions of interest (ROIs). Tc-99m MIBI SPECT was performed at 10 minutes after injection of 750 MBq of Tc-99m MIBI on 42 patients (36 males, 6 females. mean age=59.1yr) with the clinical suspicion for head and neck tumors. All lesions were histopathologically proven and data was analysed by creating ROIs over lesions and various backgrounds; submandibular gland (L/S), parotid gland (L/P). nuchal muscle (L/N), scalp muscle (L/Se). Each lesion to background ratios were calculated for each patients. Malignant disease were 37 patients and benign lesions were 5 patients, there was no statistically significant correlation between the histopathologic results and L/S ratio (p>0.05). L/P, L/N. L/Sc ratios of malignant disease and benign lesion revealed statistical difference (p<0.05). When ROC curve analysis was used, Area under the ROC curve of L/Sc ratio was largest. Sensitivity and specificity of MIBI SPECT using L/Sc ratio were 100% and 100%, respectively. It is more helpful and objective method appling L/Sc ratio to differentiating malignant from benign lesions in head and neck.

  11. 99mTc-MIBI scintigraphy and beta-2-microglobulin in patients with multiple myeloma

    International Nuclear Information System (INIS)

    Multiple myeloma is malignancy characterizing with autoimmune proliferation of malignant plasma cells. The aim of the study was to investigate the clinical usefulness of 99mTc-MIBI scintigraphy and serum beta-2-microglobulin for diagnosis, staging and therapy control in patients with multiple myeloma. 67 patients with multiple myeloma were investigated. 42 patients ware in active state and 25 patients were in remission. Planar images and/ or SPECT were performed on the rotating gamma camera (Siemens) 30 minutes and 3 hours after i.v. injection of 555-740 MBq 99mTc-MIBI. The uptake patterns were grouped as normal, diffusely increased and focal increased. Beta-2-microglobulin levels were measured by radioimmunoassay. The scintigraphy with 99mTc-MIBI was true positive in 40 patients with MM. From them, 21 patients were with diffuse uptake and 19 were with focal uptake with 29 lesions. 99mTc-MIBl marrow uptake correlated with the percentage of bone marrow plasma cells. All samples from patients in active state had a serum beta-2-microglobulin above the normal range. In two patients with false negative scan, the results were compared with the data of CT images. Positive clinical findings and increased value of tumour marker were found in these patients. One patient was with false positive scintigraphy. After therapy, the scintigraphy was true negative in 25 patients. In these patients in remission, the levels of the serum beta-2-microglobulin were near to the normal levels. In conclusion, our results demonstrated the effectiveness of both methods - 99mTc-MIBI scintigraphy and serum beta-2-microglobulin in different stage of the disease for the diagnosis, staging and therapy control by patients with multiple myeloma. (authors)

  12. Preoperative localization of parathyroid lesions in patients with primary hyperparathyroidism by {sup 99m}Tc-MIBI scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Noriyuki; Miura, Daisyu; Nakazawa, Hideki [Toranomon Hospital, Tokyo (Japan)

    2000-09-01

    The purpose of this study was to evaluate parathyroid scans obtained by early and late imaging following {sup 99m}Tc-MIBI injection. {sup 99m}Tc-MIBI scintigraphy was performed 15 min and 2 hr after intravenous injection of 300 MBq (or 370 MBq) of {sup 99m}Tc-MIBI. The subjects were 37 patients with primary hyperparathyroidism (adenoma, 34 cases; hyperplasia, 3 cases; and carcinoma 1 case) operated on between January 1994 and March 1996. Sensitivities for localization of adenoma, hyperplasia, and for the both lesions were 84%, 54%, and 76%, respectively. These results were poorer than the results of ultrasonography (US) (adenoma 91%, hyperplasia 54%, and both lesions 81%), and better than by {sup 201}TlCl/{sup 99m}Tc-O{sub 4}{sup -} subtraction scintigraphy (adenoma 69%, hyperplasia 23%, and both lesions 55%). Parathyroid gland weight and associated thyroid lesions decreased the sensitivity of localization detection using {sup 99m}Tc-MIBI. Combined use of {sup 99m}Tc-MIBI scintigraphy and US yielded higher sensitivity (89%), than obtained by either method alone. In the 53 cases operated on between April 1996 and March 2000 (adenoma, 47 cases; hyperplasia, 4 cases; carcinoma, 1 case; and diagnosis uncertain, 1 case), the sensitivities of {sup 99m}Tc-MIBI scintigraphy for the localization adenoma, hyperplasia, and for the both lesions were 83%, 20%, and 71%, respectively, versus 98%, 30%, and 85%, respectively, for US. It is possible that the light weight of parathyroid glands in the patients with hyperplasia decreased the sensitivity of late imaging. {sup 99m}Tc-MIBI scintigraphy is of high diagnostic value in terms of parathyroid function, and US in terms of morphology. The combination of {sup 99m}Tc-MIBI scintigraphy and US appeared to be the optimal diagnostic tool for localization of the lesion in hyperparathyroidism. (K.H.)

  13. The comparison of two gated SPET protocols: adenosine Tc-99m tetrafosmin and treadmill exercise Tc-99m MIBI

    International Nuclear Information System (INIS)

    The effect of adenosine and exercise on gated SPET left ventricular ejection fraction (LVEF), end diastolic volume (EDV) and end systolic volume (ESV) has not been fully investigated. The aim of the study was to compare functional measurements obtained in one-day adenosine rest and two-day stress-rest protocols in relation to ischaemia. Out of 226 consecutive patients examined with submaximal treadmill stress-rest 700 MBq Tc-99m MIBI, 26 were chosen to match those subjected to adenosine (140 μg/kg/min) enhanced by a low level exercise protocol (300 MBq and 700 MBq Tc-99m tetrofosmin for stress and rest respectively). All images were acquired on a double head system and were gated using 8 frames, 25 s per frame. ED and ES volumes increased after adenosine but decreased after treadmill resulting in the post-stress LVEF being significantly greater than after adenosine, 60 ± 11 v. 51 ± 13% (p < 0.01). This was caused by the smaller post-stress ESV in the treadmill group 40 ± 20 v. 51 ± 34, p < 0.05. In non-ischaemic scans the LVEF was greater (61± 8 v. 51 ± 14, p < 0.01) and EDV and ESV smaller after both stress and rest. The adenosine test may have an opposite influence on the EDV and ESV in comparison to the submaximal treadmill test and therefore the left ventricular function measurements after adenosine infusion should be interpreted carefully and may not represent those acquired after physical exercise. In the gated SPET scans showing ischaemia the post-stress EDV and ESV may be greater and the LVEF lower than at rest. (author)

  14. {sup 99m}Tc-MIBI scintigraphy of parathyroid adenomas and its relation to tumour size and oxyphil cell abundance

    Energy Technology Data Exchange (ETDEWEB)

    Melloul, M.; Paz, A.; Cytron, S. [Dept. of Nuclear Medicine, Hasharon Hospital, Rabin Medical Center, Petah Tikva (Israel); Koren, R.; Gal, R. [Dept. of Pathology, Hasharon Hospital, Rabin Medical Center, Petah Tikva (Israel); Feinmesser, R. [Dept. of Oto-Rhino-Laryngology, Hasharon Hospital, Rabin Medical Center, Petah Tikva (Israel)

    2001-02-01

    The aim of this study was to assess the correlation between technetium-99m methoxyisobutylisonitrile (MIBI) uptake by parathyroid adenomas, oxyphil cell content and volume of the lesions. Thirty-one patients with parathyroid adenomas were evaluated prospectively. Preoperative double-phase {sup 99m}Tc-MIBI scintigraphy was performed in all patients and tracer uptake by parathyroid lesions was assessed semi-quantitatively employing region of interest ratios to normal adjacent neck areas. Surgical specimens underwent histological evaluation and oxyphil cell content was determined. The intensity of tracer uptake was compared with oxyphil cell content, volume of the lesions and serum levels of calcium and parathormone. {sup 99m}Tc-MIBI tracer uptake was correlated with oxyphil cell content, volume of parathyroid lesions and the functional status of the parathyroid adenomas. Tracer accumulation in oxyphil cells might partially explain the preferential {sup 99m}Tc-MIBI retention in parathyroid lesions. (orig.)

  15. 99mTc-MIBI scintigraphy of parathyroid adenomas and its relation to tumour size and oxyphil cell abundance

    International Nuclear Information System (INIS)

    The aim of this study was to assess the correlation between technetium-99m methoxyisobutylisonitrile (MIBI) uptake by parathyroid adenomas, oxyphil cell content and volume of the lesions. Thirty-one patients with parathyroid adenomas were evaluated prospectively. Preoperative double-phase 99mTc-MIBI scintigraphy was performed in all patients and tracer uptake by parathyroid lesions was assessed semi-quantitatively employing region of interest ratios to normal adjacent neck areas. Surgical specimens underwent histological evaluation and oxyphil cell content was determined. The intensity of tracer uptake was compared with oxyphil cell content, volume of the lesions and serum levels of calcium and parathormone. 99mTc-MIBI tracer uptake was correlated with oxyphil cell content, volume of parathyroid lesions and the functional status of the parathyroid adenomas. Tracer accumulation in oxyphil cells might partially explain the preferential 99mTc-MIBI retention in parathyroid lesions. (orig.)

  16. The role of 99mTc-MIBI SPECT-CT in reoperation therapy of persistent hyperparathyroidism patients

    Directory of Open Access Journals (Sweden)

    Yin Lijie

    2015-01-01

    Full Text Available To compare the role of 99mTc-MIBI SPECT-CT image and US in reoperation of persistent secondary hyperparathyroidism patients. A total of 8 persistent secondary hyperparathyroidism patients underwent parathyroidectomy. The sensitivity and accuracy of US and 99mTc-MIBI images before operation were determined. 9 of 14 surgical resection tissues from 8 persistent secondary hyperparathyroidism patients were confirmed to parathyroid hyperplasia. The results showed that the sensitivities were 77.8% and 100%, respectively, for US and 99mTc-MIBI SPECT-CT images. And the accuracies of US and 99mTc-MIBI dual time planar image and SPECT-CT were 50%, 78.6%, respectively. There was significantly difference between two procedures (P=0.021. The superior and inferior localization of glands were both detected by the ultrasound and scintigraphy. Two ectopic parathyroidism nodules were found by 99mTc- MIBI SPECT-CT imaging, while US provided no consistent findings. There was no significantly difference between two procedures (P=0.3. Although two cases of them were not confirmed by pathology, the iPTH of them (800 and 1429 respectively were much higher than other four cases (<400 pg/ml. PTX is a safe and effective treatment for the patients with persistent SHPT. 99mTc-MIBI planar and SPECT-CT imaging may provide more helps for clinician’s localization the hyperparathyroidism glands accurately.

  17. Diagnostic accuracy of Tc-99m MIBI scintimammography in breast cancer

    International Nuclear Information System (INIS)

    For the characterization of breast mass, the results of Tc-99m MIBI scintimmamography were much varied between studies. This may result from various factors including tumor size, experience of observer and expression of MDR gene, So, we evaluated the influence of tumor size, knowledge of clinical information, experience of observer and expression of Pgp53 gene on the accuracy of MIBI scintimmamography for the characterization of breast mass. MIBI scintimmamography was prospectively performed in 44 patients with breast masses before surgical resection. The anterior and both lateral prone chest images were acquired at 5 min and 3 hour after injection of 740 MBq of Tc-99m MIBI. Two independent observers reviewed the images for characterization of breast mass without any clinical information. Then the images were reviewed with knowledge of clinical information including tumor size and location. If lesions proved to be malignant, presence of Pgp53 was evaluated by immunohistochemical staining. The comparisons of intraobserver/interobserver diagnostic accuracy and variance were performed by chi-square test and kappa test, respectively. The 54.4% of all lesions (24/44) were malignant. The sensitive, specificity and accuracy of scintimmamography were 73.3%, 82.8%, 79.5% and 73.3%, 84.5%, 80.7% with/without clinical information in observer A, and 76.7%, 86.2%, 83% and 70%, 89.7%, 83% with/without clinical information in observer B, respectively. There were no significant differences in sensitivity, specificity and accuracy between two observers or in same observer with/without clinical information. The agreement for the characterization of breast mass between the two observers was significantly excellent (κ=0.876/ κ=0.897 with/without clinical information, respectively). Intraobserver variance according to the knowledge of clinical information was also small ( κ=0.795 in observer A; κ=0.900 in observer B). Pgp53 was positive in 8 lesions. The sensitivities of

  18. Evaluation of some ratio effects in 99mTc-MIBI imaging of breast tumors

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The effectiveness of using some ratios in 99mTc-MIBI imaging fbr the diagnosis of breast tumors was evaluated. After 100 patients with the breast tumor underwent 99mTc-MIBI imaging, the ratios of tunor to contralateral uptake (T/N). tumor to heart uptake (T/H), and tumor to sternum uptake (T/S) were obtained and then analysed about their reproducibility and values in differentiating benign breast lesion the from malignant tumor. To detect breast cancers, the sensitivity, specificit y and accuracy of T/N were 92%, 90% and 91%, respectively. However, those of T/S were 70% (p <0.01), 74% (p <0.05), 72% (p <0.01), and those of T/H were 74%(p <0.05). 76% (p >0.05). 75% (p <0.01). The average coefticients of variation(CV) of T/N, T/S and T/H were 9.439±9.712. 4.856+4.420 (p >0.05), and 3.736±3.489 (p <0.05). It was found that T/N had the best sensitivity, specificity and accuracy todetect the breast cancer, but its reproducibility is poor. On the other hand, T/H has better reproducibility.

  19. Preparation of 186 Re-MIBI Complex for Myocardial Perfusion Imaging as Potential Replacement of Analogues 99mTc-MIBI

    International Nuclear Information System (INIS)

    The aim of the present study is to label 2-methoxy isobutyl-isonitrile (MIBI) compound with pure 186 Re and study the optimum conditions to prepare the 186 Re-MIBI complex as a stable contrast agent for myocardial perfusion imaging. From the obtained data the complexation of MIBI with 186 Re was carried out of using 1 mg MIBI, 1 mg SnCl2, 3 mg gentesic acid and 1 ml 186 Re(37 MBq) at ph 2 in a boiling water bath for 30 min. The biodistribution studies in mice indicate that, the complex was cleared from the body by kidneys to urinary bladder and finally was eliminated from the body by urine. 186 Re-MIBI demonstrated satisfactory heart uptake and retention like 99mTc-MIBI (8.94% dose/organ at 5 minutes), blood clearance was fast, while liver activity decreased by time and negligible activity in the lunges. The obtained data showed that 186 Re-MIBI as a potential replacement of 99mTc-MIBI for myocardial perfusion imaging

  20. Is Tc99m-MIBI scintigraphy a predictor of response to pre-operative neoadjuvant chemotherapy in Osteosarcoma?

    Directory of Open Access Journals (Sweden)

    Vahidreza Dabbagh Kakhki

    2013-10-01

    Full Text Available Objectives: Multidrug resistance (MDR, which may be due to the over expression of P-glycoprotein (Pgp and/or MRP, is a major problem in neoadjuvant chemotherapy of osteosarcoma. The aim of this study was to investigate the role of Tc-99m MIBI scan for predicting the response to pre-operative chemotherapy. Materials and Methods: Twenty-five patients (12 males and 13 females, aged between 8 and 52y with osteosarcoma were studied. Before the chemotherapy, planar 99mTc-MIBI anterior and posterior images were obtained 10-min [tumor-to-background ratio: (T1/B110min] and 3-hr after tracer injection. After completion of chemotherapy, again 99mTc-MIBI scan was performed at 10-min after tracer injection. In addition to calculation of decay corrected tumor to background (T/B ratios ,  using the 10-min and 3-hr images of the pre-chemotherapy scintigraphy , percent wash-out rate (WR% of 99mTc-MIBI was calculated. Using the 10-min images of the pre- and post-chemotherapy scans, the percent reduction in uptake at the tumor site after treatment (Red% was also calculated. Then after surgical resection, tumor response was assessed by percentage of necrosis. Results: All patients showed significant 99mTc-MIBI uptake in early images. Only 9 patients showed good response to chemotherapy (necrosis≥90% while 16 patients were considered as non-responder (necrosis

  1. THE CLINICAL SIGNIFICANCE OF 99mTc-MIBI BREAST IMAGING IN THE DIAGNOSIS OF EARLY BREAST CANCER

    Institute of Scientific and Technical Information of China (English)

    任长才; 金少津; 邹强; 朱汇庆; 王红鹰; 梁春立

    2001-01-01

    Objective: To find an effective, sensitive, specific and noninvasive diagnostic method of breast cancer. Methods: 109 masses of 102 patients with breast lesions smaller than 2 cm in diameter were divided into three groups to undergo 99mTc-MIBI imaging and compared with the results of pathology examination. 20 cases without breast lesions were selected as control. Abnormal condensation of 99mTc-MIBI in the breast reaching 10% higher than that in the counterpart of the healthy breast was regarded as positive. Results: Of 32 breast cancers, positive imaging appeared in 25. Negative imaging were found in 31 of 38 benign breast lesions. Of 39 occult breast lesions, positive imaging appeared in 6 and 3 of them were breast cancer, 2 of 3 patients with slightly increased 99mTc-MIBI imaging threshold were breast cancer also. No positive imaging was found in the control group. The diagnostic accuracy, sensitivity, specificity, positive predictive value, negative predictive value of 99mTc-MIBI was 88.4%, 89.2%, 88.0%, 75.0% and 95.3%, respectively. Conclusion: 99mTc-MIBI imaging had higher sensitivity and accuracy in the diagnosis of breast cancer and differentiation between benign and malignant breast lesions. It could provide useful information for the diagnosis of clinically suspected breast cancer.

  2. Evaluation of Cardiac Mitochondrial Function by a Nuclear Imaging Technique using Technetium-99m-MIBI Uptake Kinetics

    Directory of Open Access Journals (Sweden)

    Shinro Matsuo

    2013-04-01

    Full Text Available Mitochondria play an important role in energy production for the cell. The proper function of a myocardial cell largely depends on the functional capacity of the mitochondria. Therefore it is necessary to establish a novel and reliable method for a non-invasive assessment of mitochondrial function and metabolism in humans. Although originally designed for evaluating myocardial perfusion, 99mTc-MIBI can be also used to evaluate cardiac mitochondrial function. In a clinical study on ischemic heart disease, reverse redistribution of 99mTc-MIBI was evident after direct percutaneous transluminal coronary angioplasty. The presence of increased washout of 99mTc-MIBI was associated with the infarct-related artery and preserved left ventricular function. In non-ischemic cardiomyopathy, an increased washout rate of 99mTc-MIBI, which correlated inversely with left ventricular ejection fraction, was observed in patients with congestive heart failure. Increased 99mTc-MIBI washout was also observed in mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS and in doxorubicin-induced cardiomyopathy. Noninvasive assessment of cardiac mitochondrial function could be greatly beneficial in monitoring possible cardiotoxic drug use and in the evaluation of cardiac damage in clinical medicine.

  3. Role of 99mTc-MIBI in evaluation of palpable breast lesions

    International Nuclear Information System (INIS)

    Full text: Breast carcinoma is the most frequently diagnosed malignancy in women with 41% of all new cases diagnosed in developing countries. Over 323,000 women in the developing countries were detected in 1990, leading to 140,000 deaths. The annual incidence of breast carcinoma among women in Pakistan accounts for 32% of all detected carcinomas and 18% of all cancer related deaths. Three years data (2000-2002) collected from Bahawalpur Institute of Nuclear Medicine and Oncology (BINO) indicated that 16.5% of the total patients were registered with carcinoma breast. To reduce the mortality associated with this disease, screening of asymptomatic women has been advocated to diagnose problems at an early stage, particularly by X-ray mammography on annual basis after the age of 50. The available imaging modalities like mammography and ultrasound lack the desired specificity to differentiate the malignant masses from benign ones; hence there is a need to search for more specific imaging tool and to save the patients from unnecessary biopsies. Technetium-99m labeled methoxy-isobutyl-isonitrile (99mTc-MIBI) is a well-known cardiac perfusion-imaging agent. After convincing reports from various parts of the world that it specifically accumulates in malignant breast masses, we carried out a study to evaluate the diagnostic performance of 99mTc-MIBI scintimammography in palpable breast lesions. Patients with palpable breast lesions were studied after obtaining informed written consent. The study was carried out as a prospective trial. A total of 42 patients with 45 palpable breast masses were included in this study. The median age was 44 years (range 19-84 years). Tc-99m-MIBI was injected in a dose of 740 MBq in dorsal pedal vein and if unsuccessful then in medial cubital vein on the opposite side of the breast, to be imaged for the lump. Planar 10 minutes images in prone lateral and in supine position with arms raised behind the head were acquired 10 minutes and 60-90 minute

  4. Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI6]+

    Directory of Open Access Journals (Sweden)

    D.P. Faria

    2015-01-01

    Full Text Available Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [99mTc(MIBI6]+ as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol:chloroform (25:75, Whatman 3MM paper and ethanol:chloroform (25:75, and the more expensive ITLC-SG and 1-propanol:chloroform (10:90 were suitable systems for the direct determination of radiochemical purity of [99mTc(MIBI6]+ since impurities such as 99mTc-reduced-hydrolyzed (RH, 99mTcO4 - and [99mTc(cysteine2]- complex were completely separated from the radiopharmaceutical, which moved toward the front of chromatographic systems while impurities were retained at the origin. The time required for analysis was 4 to 15 min, which is appropriate for nuclear medicine routines.

  5. Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [{sup 99m}Tc(MIBI){sub 6}]{sup +}

    Energy Technology Data Exchange (ETDEWEB)

    Faria, D.P.; Buchpiguel, C.A.; Marques, F.L.N., E-mail: danielefaria1@gmail.com [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Faculdade de Medicina. Departamento de Radiologia. Servico de Medicina Nuclear

    2015-10-15

    Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [{sup 99m}Tc(MIBI){sub 6}]{sup +} as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol: chloroform (25:75), Whatman 3MM paper and ethanol:chloroform (25:75), and the more expensive ITLC-SG and 1-propanol: chloroform (10:90) were suitable systems for the direct determination of radiochemical purity of [{sup 99m}Tc(MIBI){sub 6}]{sup +} since impurities such as {sup 99m}Tc-reduced-hydrolyzed (RH), {sup 99m}TcO4{sup -} and [{sup 99m}Tc(cysteine){sub 2}]{sup -} complex were completely separated from the radiopharmaceutical, which moved toward the front of chromatographic systems while impurities were retained at the origin. The time required for analysis was 4 to 15 min, which is appropriate for nuclear medicine routines. (author)

  6. Preoperative localization of parathyroid lesions. Value of {sup 99m}Tc-MIBI tomography and factors influencing detection

    Energy Technology Data Exchange (ETDEWEB)

    Ansquer, C.; Carlier, T.; Kraeber-Bodere, F. [Hotel Dieu Univ. Hospital, Nantes (France). Nuclear Medicine Dept.; Mirallie, E. [Hotel Dieu Univ. Hospital, Nantes (France). Surgery Dept.; Abbey-Huguenin, H. [Rene Gauducheau Centre, Saint Herblain (France). Biostatistics Dept.; St Jacques Univ. Hospital, Nantes (France). PIMESP; Aubron, F. [Ultrasonography Centre, Nantes (France)

    2008-07-01

    The aim of our study was to assess retrospectively the value of {sup 99m}Tc-MIBI SPECT in the localization of parathyroid lesions in primary hyperparathyroidism and to determine the impact of PTH level, age, sex, characteristics of the lesions and thyroid nodules on the sensitivity of imaging. Patients, methods: Fifty nine patients who were cured after the resection of 60 lesions (50 adenomas, 9 hyperplasias and 1 carcinoma, 9 of them in ectopy) were selected. {sup 99m}TcO{sub 4}{sup -}, early and late {sup 99m}Tc-MIBI planar images (n=59), {sup 99m}Tc-MIBI SPECT (n=58) and ultrasound (n=50) performed preoperatively were analyzed. The imaging results were compared to surgical and histological findings and correlated to different factors suspected of influencing the imaging's sensitivity. Results: Sensitivity of double phase {sup 99m}Tc-MIBI/{sup 99m}TcO{sub 4}{sup -} scintigraphy was higher than that of early or late scintigraphy alone. SPECT increased the sensitivity of scintigraphy from 85% to 92% and was useful to confirm doubtful foci and to localize ectopic lesions. Ultrasound (US) had the lowest sensitivity (56%) and the highest rate of false-positive results (n=10), but identified 2 adenomas which were not detected by scintigraphy. Combining all imaging modalities, sensitivity reached 96%. Better sensitivities were observed when age <69 years, preoperative PTH level {>=}155 pg/ml, weight of the gland {>=}0.80 g and in the absence of thyroid nodules. US was more influenced by these factors than scintigraphy. Conclusion: Combination of US, double-phase {sup 99m}Tc-MIBI/{sup 99m}TcO{sub 4}{sup -} planar scintigraphy and SPECT is the most accurate method for the detection of parathyroid lesions and should be performed before minimally invasive surgery, especially when PTH level is low, in older patients and in cases of multinodular goiter. (orig.)

  7. Extra cardiac activity detected on myocardial perfusion scintigraphy after intra-arterial injection of 99mTc-MIBI

    DEFF Research Database (Denmark)

    Afzelius, Pia; Henriksen, Jens H

    2008-01-01

    , prolongation of the study and interference of the extra cardiac activity with the cardiac image reconstructions. Whole-body scintigraphy disclosed an arterial flow distribution of activity to skeletal muscles in left shoulder and upper limb. CONCLUSION: Accidentally injected radiotracer retrogradely......PURPOSE: We present an unusual case of extra cardiac activity of 99mTc-MIBI in the left part of thorax and left upper extremity in a patient admitted for myocardial perfusion scintigraphy. METHODS: A standard 2-day protocol of 99mTc-MIBI perfusion scintigraphies starting day 1 with stress...... (dipyridamol) imaging and followed by rest imaging day 2 was performed. RESULTS: On day 2, when rest perfusion scintigraphy was carried out, extra cardiac activity was present in the left part of thorax and in the left upper extremity resulting in reduced accumulation of 99mTc-MIBI in cardiac tissue...

  8. Non-visualized Thyroid Gland by Tc-{sup 99m} MIBI Scan with Normal Thyroid Scan

    Energy Technology Data Exchange (ETDEWEB)

    Koca, Gokhan; Atilgan, Hasan Ikbal; Baskin, Aylin; Demirel, Koray; Korkmaz, Meliha [Ankara Training and Research Hospital, Ankara (Turkmenistan)

    2013-09-15

    We present the case of a 21-year-old man who was referred to us for parathyroid scintigraphy with high blood levels of intact parathormone and osteoporosis. Several methods and radiopharmaceuticals, e.g., Tc-99m MIBI and Tl-201 chloride/Tc-99m pertechnetate (Tl-201/TcPO{sup -4}) subtraction, are commonly used for this purpose. We present the case of a thyroid gland that demonstrates quite normal Tc-99m pertechnetate uptake, no accumulation of Tc-99m MIBI, and very low grade Tl-201 uptake. To the best of our knowledge, no similar case has been reported previously. A 21-year-old male with osteoporosis and growth-development retardation was referred for MIBI parathyroid scan because of high blood levels of intact parathormone and bone-specific alkaline-phosphatase, which were 219.4 (15-88 pg/ml) and 355 (21-58 U/L), respectively. In his Tc-99m pertechnetate (TcPO{sup -4}) pinhole scintigraphy, bilateral clearly visualized radioactivity accumulation in the thyroid gland was seen. In both early or late images of the Tc-99m MIBI parathyroid scan, the thyroid gland was not visualized. Therefore, a Tl-201/TcPO{sub -4} subtraction scan method was used. However, the Tl-201 accumulation level in the thyroid gland was not sufficient for the subtraction method. In his thyroid ultrasonography, the thyroid gland echo was homogenous, and there was neither any solid nor cystic lesion. The physical examination of his neck was normal. Other laboratory findings were all normal as follows. TSH: 3.03 (0.35-5.6 IU/mL), free T3: 3.66 (2.5-3.9 pg/mL), free T4: 0.90 (0.59-1.3 ng/dL), Anti-TPO:0.3 (0.40 IU/mL), Anti-TG-Ab: <2.2 (0-+u/L), TSH receptor Ab: 1.0 (0.14 U/L), osteocalcine: 9.13 (1.5-15 ng/dL), growth hormone: 1.3 (0.014-5.21), calcitonin:17 (0.150 ng/mL), sedimentation:6 (0.15 mm/h). There were no significant symptoms of acute or chronic thyroiditis. The cause for discordant uptake in the thyroid gland with T1-201 and Tc-99m MIBI scan could not be provided through clinical or

  9. Localization of parathyroid adenoma by echography and scintigraphy with 99mTc-MIBI: the influence of lesion's weight

    International Nuclear Information System (INIS)

    In the examination for pre-surgery localization of a primary hyperparathyroidism (HPT I), the size of parathyroid lesion constitutes the main factor limiting the diagnostic efficiency of different imaging techniques. We have tried to test this hypothesis by echography and scintigraphy with MIBI for the detection of unique adenomas. The study covered 16 patients explored pre-surgically by echography and scintigraphy with 99mTc-MIBI for a HPT I as referred to a unique cervical adenoma (reference = surgery + anatomical-pathological examination). The scintigraphy with MIBI is achieved and interpreted by the 'double phase' technique proposed by Taillefer with pin-hole acquisition of 15 and 120 minutes after injection of around 370 MBq of 99mTc-MIBI. The average weight of adenoma is 3180 mg (218 to 22000). The echography localizes correctly the adenoma in 8 cases, in a wrong position, in 2 cases and does not find images evoking abnormal parathyroid in 6 patients. The MIBI visualizes properly the adenoma in 15 cases and in an incorrect position, in 1 patient. A table is given presenting the echography and MIBI-scintigraphy sensitivities as a function of adenoma weights. In conclusion, in our study the MIBI appear to be more sensitive than the echography in localizing the parathyroid adenoma. The diagnostic value of the two examinations appears to be independent of its weight; however, the results should be confirmed on larger batches

  10. Correlation between P-glycoprotein (P-gp) expression in parathyroid and Tc-99m MIBI parathyroid image findings

    Energy Technology Data Exchange (ETDEWEB)

    Sun, S.-S.; Shiau, Y.-C.; Lin, C.-C.; Kao, Albert E-mail: albertkaotw@yahoo.com.tw; Lee, C.-C

    2001-11-01

    The major factor to influence localization of parathyroid adenomas is tumor size. P-glycoprotein (P-gp) expression in parathyroid adenomas has been considered to be an another possible factor to influence localization of parathyroid adenomas because false-negative studies have been reported with large tumors and true-positives reported with very small tumors in previous studies. The aim of this study was to characterize Tc-99m MIBI uptake and retention by parathyroid adenomas and to correlate this with cell surface expression of P-gp. Sixteen patients with parathyroid adenoma (larger than 1.5 gm) underwent dual-phase (10min and 2hr) Tc-99m MIBI parathyroid image immediately before parathyroid exploration. Tissues were obtained from normal and abnormal parathyroid glands and from the thyroid gland. Immunohistochemistry (IHC) was obtained with monoclonal antibodies to identify P-gp expression in all tissues. All of the 16 parathyroid adenomas and 32 normal control specimens (16 normal parathyroid and 16 normal thyroid specimens) were submitted for P-gp detection by IHC. The dual-phase Tc-99m MIBI parathyroid image accurately localized 14 parathyroid adenomas, but not the remaining 2 adenomas. The 14 parathyroid adenomas with significant Tc-99m MIBI uptake in delayed 2hr images revealed negative P-gp expression, but the 2 adenomas without significant Tc-99m MIBI uptake, as well as normal parathyroid and normal thyroid specimens, revealed positive P-gp expression when evaluated by IHC. Not only the size of parathyroid adenomas, but also significant P-gp expression limited the sensitivity of dual-phase Tc-99m MIBI parathyroid image to localize parathyroid adenomas before operation.

  11. 99mTc-MIBI SPECT in primary hyperparathyroidism. Influence of concomitant vitamin D deficiency for visualization of parathyroid adenomas

    International Nuclear Information System (INIS)

    Aim of the study was to analyse the influence of a concomitant vitamin D deficiency on the results of 99mTc-MIBI studies in patients (pts) with primary hyperparathyroidism (pHPT). Patients, methods: between January 1998 and May 2004, 71 pts with pHPT had undergone operation after a 99mTc-MIBI study of whom 54 pts (76%) had normal values of 25-OH-vitamin D3 and 17 pts (24%) had vitamin D deficiency. Results of a dual-phase 99mTc-MIBI protocol with SPECT were compared with histopathology. Results: in 54 pts with normal vitamin D values late SPECT images identified more lesions (n 51, sensitivity 91%) than early planar (n = 45, sensitivity 82%) or late planar images (n = 50, sensitivity 88%). In 17 pts with vitamin D deficiency late SPECT images identified more lesions (n = 13, sensitivity 72%) than early planar (n = 10, sensitivity 56%) or late planar images (n = 10, sensitivity 56%) too. In pts with vitamin D deficiency the sensitivity of a 99mTc-MIBI SPECT study was lower than in those with normal vitamin D status (72% vs. 91%) and dependent on the value for PTH. However, the results did not reach statistical significance: early planar: p = 0.1625; late planar: p = 0.0039; 99mTc-MIBI SPECT: p 0.1180. Conclusion: the likelihood of a pathological 99mTc-MIBI study being obtained in pts with pHPT is dependent on the parathyroid hormone level. However, a negative influence of a low vitamin D level on the scintigraphic detection rate of a parathyroid adenoma could not be proven which may be due to the low number of pts with vitamin D deficiency. (orig.)

  12. 99mTc-MIBI进行鼻咽癌显像的临床研究%Detection of nasopharyngeal carcinoma with Tc-99m MIBI

    Institute of Scientific and Technical Information of China (English)

    杜建强; 裴庆华; 岳殿超; 曾世荃

    2004-01-01

    Objective It is difficult to differentiate primary and recurrent nasopharyngeal carcinoma (NPC). Using 99mTc MIBI scintigraphy, we study patients with NPC in order to determine the efticacy of 99mTc MIBI scintigraphy in detecting the primary, recurrent and metastatic tumors. Method NPC patients were divided into two groups, primary (n= 43) and recurrent (n= 17). 99mTc MIBI SPECT study of head and neck and whole body scan to detect the primary, recurrent and metastases foci were performed on both groups as well as on volunteers (n = 21). Comparing with the scalp, the uptakes on the nasopharynx were divided into 5 grades, assessed by 3 physicians independently without any clinical information. Results In the primary NPC group, 36 patients were positive and 5 were negative. 13 patients had metastases in neck lymph nodes and 2 had lungs metastases. The specificity was 90.5 % ( 19 of 21) and the sensitivity was 82.7 % (36 of 43 ). In the recurrent group, 13 patients were positive, and 4 were negative. The sensitivity was 76.5 % ( 13 of 17 ). Conclusion This study suggests that 99mTc MIBI SPECT is a useful method for detecting NPC with particular potential value in diagnosis of recurrent NPC.%目的在临床上,鼻咽癌复发的诊断是非常困难的,我们用99mTc-MIBI来探索鼻咽癌的原发灶、复发灶和转移灶的诊断.方法用99mTc-MIBI断层显像检测21例对照组和41例治疗前鼻咽癌病例,病灶的放射性摄取与头皮比较,由3名以上的医师在不知道任何临床情况下分别阅片.结果41例治疗前鼻咽癌病例中共有36例阳性,特异性为90.5%,灵敏度为83.7%.结论99mTc-MIBI显像对鼻咽癌原发灶和转移灶的诊断,是一种灵敏的方法,值得进一步探讨.

  13. Labelling, biodistribution and compartmental analysis of N-acetylcysteine labelled with Tc-99m. Comparative investigation with with {sup 99m} Tc-MIBI in an in vivo tumoral model; Estudo de marcacao, biodistribuicao e analise compartimental da N-acetil cisteina marcada com Tc-99m. Investigacao comparativa com MIBI-{sup 99m}Tc em modelo tumoral in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Faintuch, Bluma Linkowski

    1997-07-01

    Labelling and biodistribution studies were done with two different ligands, respectively Methoxy isobutyl isonitrile (MIBI) and N-acetylcysteine (NAC), employing Tc-99m as a tracer. The main objective was to assess the pharmacokinetic properties of the second substance, aiming at its possible application in cancer diagnosis. To this purpose an in vivo investigation was done using healthy and tumor-bearing rats with experimental cancer. Images of tumor-bearing rats registered in a scintillation camera indicated that with {sup 99m} Tc-MIBI none of the two selected times was adequate for visualization of the cancer mass. In contrast, {sup 99m} Tc-NAC permitted clear identification of the humor, four hours after injection. The results have demonstrated that {sup 99m} Tc-NAC is a radiopharmaceutical with affinity for cancer tissue and promising for further investigation concerning imaging diagnosis of tumors. (author)

  14. Effect of P-glycoprotein and multidrug resistance associated protein gene expression on Tc-99m MIBI imaging in hepatocellular carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Chang, C.-S. E-mail: changcs@vghtc.gov.tw; Huang, W.-T.; Yang, S.-S.; Yeh, H.-Z.; Kao, C.-H.; Chen, G.-H

    2003-02-01

    P-glycoprotein (Pgp) and multidrug resistance-associated protein (MRP) expressions as well as Tc-99m methoxisobutylisonitrile (MIBI) images were assessed in 25 patients hepatocellular carcinoma (HCC). Tc-99m MIBI imaging was performed 10 minutes after intravenous injection of 20 mCi Tc-99m MIBI. Using immunohistochemical staining, 60% of the HCC lesions showed positive for Pgp and 64% showed positive for MRP. In 3 patients with MIBI uptake, immunohistochemical study of tumor tissue showed no Pgp stained cells. Nevertheless, they were all positive for MRP. The result of Tc-99m MIBI imaging is more related to the expression of Pgp than MRP gene. It is possible that other membrane transporters as well as Pgp and MRP are involved in the efflux of Tc-99m MIBI.

  15. Combined Use of Tc-99m MIBI Scan and MRI for Evaluation of Active Lesions in Multiple Myeloma

    International Nuclear Information System (INIS)

    Full text: Multiple Myeloma is a malignancy of plasma cells, in which neoplastic bone involvement is one of the leading symptoms. Many researchers have been trying to develop new imaging modalities and new methods for detecting the bone involvement, mainly because apart from the fact that bone marrow infiltration is one of the diagnostic criteria, typical radiographic findings can be found only in 60 70% of pts with first diagnosis of MM, and 10% of pts can present only osteoporosis-like changes. Purpose To evaluate the efficacy of 99mTc-MIBI whole-body scan in diagnosis of Multiple Myeloma, in correlation with other imaging modalities, and mainly with MRI. Material and Method: We performed a retrospective review of 29 cases of Multiple Myeloma Patients, all of them in active stage of the disease. All of them underwent 99mTc-MIBI whole body scan, MRI examination of the spine and a bone scan. Diagnosis and staging of the disease was made by using standard criteria. MRI was performed mainly for imaging spinal cord compression and for morphological characterization of lesions. We obtained saggital and transverse images which included T1-weighted spin-echo, T2-weighted turbo spin-echo and STIR sequences. We performed whole body MIBI scan in anterior and posterior views, 10 - 20 min after IV injection of 500-550 MBq of 99mTc-MIBI, using a dual head gamma-camera Philips- Axis. Results: We obtained pathologic changes in 25/29 pts that underwent whole-body MIBI scan(86.75%), and in 27/29(89.65%) for the MRI. Bone scan shown pathologic uptake of radiotracer in 18/29 pts(62.06%). In 4 pts that demonstrated pathologic increased uptake of MIBI we found photopenic lesions on the bone scan which were missed at the first interpretation of the bone scan; these lesions were reported only after comparative evaluation of bone scan and MIBI scan.We found three different patterns of pathologic MIBI uptake: focal increased uptake of MIBI in different sites (9 pts), diffuse increased

  16. Parathyroid glands: combination of (99m) Tc MIBI scintigraphy and US for demonstration of parathyroid glands and nodules

    OpenAIRE

    Colagrande, S; C. Biagini; BISI, G; L.Vaggelli; D. Borrelli; P. Cicchi; TONELLI, F; Amorosi, A; M.Serio; De Feo, M.L; Brandi, M.L.

    2000-01-01

    PURPOSE: To determine the appropriate choice of imaging techniques for localization of nodular lesions of parathyroid glands. MATERIALS AND METHODS: First, computed tomographic (CT), magnetic resonance (MR), ultrasonographic (US), and technetium 99m methoxyisobutyl-isonitrile (MIBI) scintigraphic images in 49 patients with primary hyperparathyroidism were retrospectively evaluated. A single-blind, prospective study that included 16 patients with primary hyperparathyroidism was then condu...

  17. Absorbed radiation to the nuclear medicine nurses from patients administered 201Tl and 99mTc- MIBI

    International Nuclear Information System (INIS)

    People who have administrated radiopharmaceuticals could be a source of radiation to their relatives, medical nurses, and people who have contact them. In this study, the dose rates at various distances of 5, 10,50 and 100cm from 70 patients, who were administered diagnostic amounts of 201Tl-Chloride and 99mTc-MIBI, was measured using an ionization chamber. For determination of external radiation dose to the nurses the radiations in three deferent interval times have measured. The maximum values of external dose rates of 201Tl and 99mTc- MIBI were 11.2μSv/h ±2.3 and 43.1μSv/h ±11.9 respectively at 5cm from the patients. Significant exposure from patients after injection of 99mTc-MIBI was limited on the day of administration. Departure doses rate of 201Tl fell gradually so by 3 days after administration was significant. Maximum and average absorbed dose of nuclear medicine staff for one 201Tl scan was 4.6 and 2.7μSv/h, and for 99mTc-MIBI was 18.1 and 9.8μSv/h in a working day. (author)

  18. Contribution of the Technetium-99m-sestamibi (MIBI) scintigraphy in primary hyperparathyroidism to determination of parathyroidectomy

    Energy Technology Data Exchange (ETDEWEB)

    Nishida, Takuro; Hayakawa, Katsumi; Nishimura, Kazumasa; Kawakami, Satoshi; Sakamoto, Makoto; Koh, Toshikiyo; Hida, Shuichi [Kyoto City Hospital (Japan)

    1999-02-01

    We reviewed retrospectively the 12 patients who underwent parathyroidectomy because of hyperparathyroidism and judged to what degree the Technetium-99m-sestamibi (MIBI) scintigraphy contributed to determination of parathyroidectomy. From the view point that weather parathyroidectomy could be decided without the MIBI scintigraphy, we classified the cases into three groups; great, fair, and poor contribution. We judged 4 cases as great contribution, 5 cases as fair contribution and 3 cases as poor contribution. We concluded that if the MIBI scintigraphy is positive in patients with primary hyperparathyroidism, parathyroidectomy is strongly recommended, because of its high sensitivity and accuracy for detection of parathyroid adenomas and because there were no false-positive cases with the MIBI scintigraphy. (author)

  19. Multiple myeloma: predictive value of Tc-99m MIBI scintigraphy and MRI in its diagnosis and therapy

    International Nuclear Information System (INIS)

    We assessed the validity of 99mTc-MIBI scintigraphy and MRI in the diagnosis and prediction of the effect of therapy in patients with multiple myeloma (MM) and monoclonal gammopathy of unknown significance (MGUS), in whom both examinations were performed within 14 days. Forty-seven consecutive patients with MM and 5 with MGUS were enrolled in the study. Out of 47 MM patients, 6 were in Durie-Salmon stage I and 41 had active disease in stage II or III. Fifteen patients were examined before and within 2 months of intensive chemotherapy. Anterior and posterior whole-body scans were obtained 10 min after IV administration of 740 MBq (20 mCi) 99mTc-MIBI. MRI of Th and LS spine, T1 w.i. and STIR in the sagittal plane were performed. Bone marrow pathological changes in 41 MM patients with active disease were detected in 39 (95%) scintigraphic examinations and in 38 (94%) of MRI. Among 41 MM patients with active disease, 21 showed diffuse patterns of 99mTc-MIBI uptake, 8 showed focal patterns and 10 showed both focal and diffuse patterns, while 34 patients exhibited focal lesions in MRI and 4 both focal and diffuse findings. Moreover, 5 of 38 patients had epidural mass and 18 had vertebrae compression. Out of 15 patients after therapy, 13 reached complete remission and 2 had stable disease. Normal 99mTc-MIBI scintigraphy was found in 11 (85%) patients with complete remission, 2 presented both focal and diffuse patterns of 99mTc-MIBI uptake. Two patients with stable disease also had focal and diffuse radiotracer uptake. MRI findings were normal only in 3 (23%) patients in complete remission. Eight patients exhibited focal lesions and 2 showed partial conversion in MRI. 99mTc-MIBI scintigraphy and MRI are methods of equal sensitivity in detecting active MM and complement each other. The advantage of 99mTc-MIBI scintigraphy is the possibility of whole body examination, which allows superiority in detection of MM in appendicular skeleton and extramedular lesions, and faster

  20. {sup 99m}Tc-MIBI pinhole SPECT in primary hyperparathyroidism: comparison with conventional SPECT, planar scintigraphy and ultrasonography

    Energy Technology Data Exchange (ETDEWEB)

    Carlier, Thomas; Bodet-Milin, Caroline; Kraeber-Bodere, Francoise; Ansquer, Catherine [Hotel Dieu University Hospital, Nuclear Medicine Department, Nantes (France); INSERM CRCNA, Nantes (France); Oudoux, Aurore [Hotel Dieu University Hospital, Nuclear Medicine Department, Nantes (France); Mirallie, Eric [Hotel Dieu University Hospital, Surgery Department, Nantes (France); Seret, Alain [University of Liege, Institute of Physics, Experimental Medical Imaging, Liege (Belgium); Daumy, Isabelle [Ultrasonography Center, Nantes (France); Leux, Christophe [Saint Jacques University Hospital, PIMESP, Nantes (France)

    2008-03-15

    A pinhole collimator is routinely used to increase the resolution of scintigraphy. This prospective study was conducted to determine the interest of {sup 99m}Tc-MIBI pinhole single-photon emission computed tomography (SPECT) for the preoperative localisation of parathyroid lesions in primary hyperparathyroidism. All patients underwent a neck ultrasonography (US), {sup 99m}TcO{sub 4}{sup -} and {sup 99m}Tc-MIBI planar images and two consecutive SPECT with a parallel (C-SPECT) and a pinhole collimator (P-SPECT). P-SPECT was performed with a tilted detector equipped with a pinhole collimator and reconstructed with a dedicated OSEM algorithm. A diagnostic confidence score (CS) was assigned to each procedure considering intensity and extra-thyroidal location of suspected lesions: 0 = negative, 1 = doubtful, 2 = moderately positive, 3 = positive. The results of these preoperative localisation studies were compared with surgical, pathological and 6-month biological findings. Fifty-one patients cured after surgery were included. Surgery revealed 55 lesions (median weight 0.5 g, 11 in ectopy). Sensitivities of US, planar imaging, C-SPECT and P-SPECT were, respectively, 51, 76, 82 and 87%. Nine glands were only detected by tomography and five glands only by P-SPECT. {sup 99m}Tc-MIBI/{sup 99m}TcO{sub 4}{sup -} planar scans and P-SPECT were complementary and, when combined together, showed the highest sensitivity (93%). Compared with planar imaging and C-SPECT, P-SPECT increased CS for 42 and 53% of lesions, respectively, and contributed to markedly reduce the number of uncertain results. A combination of planar {sup 99m}Tc-MIBI/{sup 99m}TcO{sub 4}{sup -} scintigraphy and P-SPECT appears to be a highly accurate preoperative imaging procedure in primary hyperparathyroidism. (orig.)

  1. 99mTc-MIBI Washout Rate to Evaluate the Effects of Steroid Therapy in Cardiac Sarcoidosis

    Directory of Open Access Journals (Sweden)

    Masayoshi Sarai

    2013-10-01

    Full Text Available Objective: We sought to determine the usefulness of the 99mTc-MIBI (MIBI washout rate for the evaluation of steroid therapy in cardiac sarcoidosis (CS. Methods: Eleven CS patients underwent MIBI myocardial SPECT both before and 6 months after initiating steroid therapy. The washout rate (WOR of MIBI was calculated using early and delayed polar map images. The washout score (WOS of MIBI was derived from the difference between the early and delayed total defect scores (TDS. Results: Serum ACE and BNP exhibited significant improvement after the therapy (p = 0.004, p = 0.045. In the LV function, EDV and E/A ratio exhibited significant improvement after the therapy (p = 0.041, p = 0.007, while there were no significant differences between before and after therapy in EF or ESV. Early and delayed TDS showed no significant differences between before and after the therapy. In contrast, WOR differed significantly (p < .0001, while WOS did not differ significantly between before and after the therapy.Conclusion: The washout rate of MIBI is suitable for assessment of cardiac function in CS with steroid therapy, being especially better than the washout score of MIBI for assessment of disease activity of mild myocardial damage in CS with steroid therapy.

  2. 99mTc-(V)DMSA scintimammography in the assessment of breast lesions: comparative study with 99mTc-MIBI

    International Nuclear Information System (INIS)

    The purpose of this study was to evaluate and compare the diagnostic accuracy of pentavalent technetium-99m dimercaptosuccinic acid [99mTc-(V)DMSA] and 99mTc-methoxyisobutylisonitrile (MIBI) in the detection of primary breast cancer and metastatic lymph node involvement, and in the clarification of cases with indeterminate mammograms. Forty-one women (mean age±SD 55±7 years) referred for a suspicious breast lesion on physical examination and/or an abnormal mammogram underwent MIBI and (V)DMSA scintimammography (SMM) at separate sessions (48-h interval). Lateral prone and anterior supine images were obtained at 10 and 60 min after administration of 740-925 MBq of each tracer, in the arm contralateral to the breast lesion. The ipsilateral axillary region was also included in the field of view. The results of SMM and mammography were compared with histological findings. Breast cancer was histologically confirmed in 26 patients (mean diameter±SD 2.87±1.5 cm). Benign lesions were found in 15 patients (mean diameter±SD 2.04±2.7 cm). Mammography was definitely positive in 23/26 patients with breast cancer and indeterminate in 3/26 (sensitivity 88.4%). In benign lesions, mammography was true negative in 5/15 cases and indeterminate in 10/15 (specificity 33.3%). Both MIBI and (V)DMSA SMM detected 23/26 breast cancers (sensitivity 88.4%) and were true negative in 14/15 (specificity 93.3%). T/B ratios for breast cancer in MIBI and (V)DMSA scans were similar, and significantly higher than for benign lesions. MIBI correctly diagnosed 12/13 and (V)DMSA 11/13 cases in which the findings of mammography were indeterminate. In addition, (V)DMSA detected seven of eight cases of in situ ductal carcinoma (DCIS) associated with infiltrating carcinomas, while MIBI detected only two of these eight cases. (V)DSMA was also diffusely concentrated in benign lesions complicated by epithelial hyperplasia. Metastatic lymph node involvement was successfully imaged in 15/19 patients with

  3. 99mTc-YIGSR as a Receptor Tracer in Imaging the Ehrlich Ascites Tumor-bearing Mice as Compared with 99mTc-MIBI

    Institute of Scientific and Technical Information of China (English)

    HU Jia; QIN Guangming; ZHANG Yongxue; AN Rui; LAN Xiaoli

    2007-01-01

    The validity of 99mTc-YIGSR, a novel receptor radio-tracer, in imaging the Ehrlich ascites tumor was evaluated. YIGSR, a pentapeptide of laminin, was labeled with 99mTc by using a bifunctional chelator S-Acetly-NH3-MAG3. The MIBI was labeled with 99mTc by following the kit instruction. The mice of tumor group were intravenously injected 1-2 mCi of 99mTc-YIGSR or 99mTc-MIBI via caudal vein, immobilized and imaged under a Gamma camera. The same procedure was performed in mice of blockade group, in which the unlabeled YIGSR was previously injected to block the receptor-recognition sites, and inflammation group serving as control. The reverse-phase Sep-Pak C18 chromatogram was found to have an essentially complete conjugation between YIGSR and S-Acetly-NH3-MAG3. The conjugated YIGSR could be radio-labeled successfully with 99mTc at room temperature and neutral pH, with a radio-labeling yield of 62%. Without the chelator S-Acetly-NH3-MAG3, the YIGSR was labeled with 99mTc at an efficiency of 4%. The imagological study revealed obvious tumor accumulation of 99mTc-YIGSR 15 min after the injection, and the uptake peaked after 3 h with a tumor-to-muscle ratio (T/M) of 11.36. The radio-tracer was slowly cleared up and resulted in a T/M of 3.01 at the 8th h after the injection. As for blocked group, the tumor uptake of radiotracer was significantly lower, with the highest T/M being 4.61 after 3 h and 0.89 after 8 h. The T/M was 3.72 at the 3rd h and 1.29 at the 8th h after the 99mTc-YIGSR injection in the inflammatory group. The T/M was significantly higher in tumor group than in inflammatory group or control group (P<0.001). In the 99mTc-MIBI group, the T/M was 1.40 at the 3rd h and 0.55 at the 8th h after the injection, which showed a significant difference as compared with 99mTc-YIGSR (P<0.001).It is concluded that YIGSR can be successfully radiolabelled by using S-Acetly-NH3-MAG3.99mTc-YIGSR has many advantages in tumor imaging, such as quick and clear visualization

  4. Effect of verapamil on cellular uptake of Tc-99m MIBI and tetrofosmin on several cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dae Hyun; Yoo, Jung Ah; Bae, Jin Ho; Jeong, Shin Young; Suh, Myung Rang; Ahn, Byeong Cheol; Lee, Kyu Bo; Lee, Jae Tae [School of Medicine, Kyungpook National Univ., Daegu (Korea, Republic of)

    2004-02-01

    Cellular uptake of {sup 99}mTc-sestamibi (MIBI) and {sup 99}mTc-tetrofosmin (TF) is low in cancer cells expressing multidrug resistance(MDR) by p-glycoprotein(Pgp) or multidrug related protein(MRP). Verapamil is known to increase cellular uptake of MIBI in MDR cancer cells, but is recently reported to have different effects on tracer uptake in certain cancer cells. This study was prepared to evaluate effects of verapamil on cellular uptake of MIBI and TF in several cancer cells. Cellular uptakes of Tc-99m MIBI and TF were measured in erythroleukemia K562 cell, breast cancer MCF7 cell, and human ovarian cancer SK-OV-3 cells, and data were compared with those of doxorubicin-resistant K562(Ad) cells. RT-PCR and Western blot analysis were used for the detection of mdr1 mRNA and Pgp expression, and to observe changes in isotypes of PKC enzyme. Effects of verapamil on MIBI and TF uptake were evaluated at different concentrations upto 200 {mu}M at 1*10{sup 6} cells/{sup m}l at 37.deg.C. Radioactivity in supernatant and pellet was measured with gamma counter to calculate cellular uptake ratio. Toxicity of verapamil was measured with MTT assay. Cellular uptakes of MIBI and TF were increased by time in four cancer cells studied. Co-incubation with verapamil resulted in an increase in uptake of MIBI and TF in K562(Adr) cell at a concentration of 100 {mu}M and the maximal increase at 50 {mu}M was 10-times to baseline. In contrast, uptakes of MIBI and TF in K562, MCF7m SK-OV3 cells were decreased with verapamil treatment at a concentration over 1 {mu}M. With a concentration of 200 {mu}M verapamil, respectively. Cellular uptakes of MIBI and TF in MCF7 and SK-OV-3 cells were not changed with 10{mu}M, but were also decreased with verapamil higher than 10{mu}M, resulting 40% and 5% of baseline at 50 {mu}M. MTT assay of four cells revealed that K562, MCF7, SK-OV3 were not damaged with verapamil at 200 {mu}M. Although verapamil increases uptake of MIBI and TF in MDR cancer cells

  5. The value of Tc-99m MIBI scintigraphy in active disease and remission phase of multiple myeloma

    International Nuclear Information System (INIS)

    Background: 99m Tc methoxy isobutyl isonitrile (99m Tc -MIBI)has been proposed as a tumor seeking agent in malignant disease. The goal of this study is to evaluate the frequency distribution of the different patterns, intensity and extension of abnormal uptake identified in MIBI scan in relation with various clinical status of the patients diagnosed as a multiple myeloma. Methods: forty-three patients entered the study, including six patients with no prior treatment , 22 patients who received autologous bone morrow graft, and 15 patients with history of chemotherapy and radiotherapy. Plasma protein electrophoresis for monoclonal antibody, bone morrow biopsy and urine analysis for Bence-Jones protein has been carried out and standard criteria were used for diagnosis of active disease and remission phase for each patients. The extension of each lesion(E-score) on scintigraphy were categorized into E0-E3 by three nuclear physicians who were blinded to the patient's clinical condition. I-score was also obtained with comparing the intensity of the lesions with intensity of myocardial uptake and classified as I0-I3. Results: the sensitivity, specificity, positive predictive value and negative predictive value of 99m Tc -MIBI scan for determining active lesions and released cases were 69%, 100%, 100% and 60%, respectively. Nineteen patients were initially thought to be in remission phase, but scintigraphy was abnormal in 5 cases who were diagnosed as active myeloma later in the course of the study. There was a significant correlation between clinical status and pattern, intensity and extension of the abnormal uptake of 99m Tc -MIBI. Also a significant correlation between intensity and extension of the abnormal tracer uptake with serum monoclonal component and urine Bence-Jones protein was noted, however no correlation between blood hemo globulin and degree of extension in scintigraphy was seen. Conclusion: Our study suggests the pattern, extension and intensity of 99m Tc

  6. Clinical Investigation of 99mTc-MIBI Imaging in Head and Neck Tumors%头颈部肿瘤99mTc-MIBI阳性显像的临床研究

    Institute of Scientific and Technical Information of China (English)

    刘志军; 张永学; 张萍; 张继青

    2006-01-01

    Objective: To investigate the diagnostic potential value of 99mTc-MIBI imaging in head and neck tumors. Methods: Ninety-one patients with malignant and benign head and neck lesions were subjected to99mTc-MIBI tomography, and 20 healthy volunteers served as control group. Results: The overall sensitivity, specificity, accuracy and positive predictive accuracy of early/delay 99mTc-MIBI imaging in diagnosis of head and neck malignant tumors were 78.7%/72.3%, 72.1%/88.4%, 75.6%/80.0% and 75.5%/87.2%respectively. The results of 99mTc-MIBI Imaging in diagnosis of nasopharyngeal cancer and sinonasal non-Hodgkin's lymphoma were more satisfactory than those in maxillary sinus cancer. In distinguishing recurrent/residual nasopharyngeal carcinoma and sinonasal non-Hodgkin's lymphoma, 99mTc-MIBI scintigraphy was superior to CT/MRI. Conclusion: 99mTc-MIBI imaging is a promising useful tool in identifying head and neck tumor, and it has a special value to evaluate the local invasion and metastasis involved.

  7. Effect of multidrug resistance gene-1(mdr1) overexpression on in-vitro uptake of {sup 99m}Tc-sestaMIBI in murine L1210 leukemia cells

    Energy Technology Data Exchange (ETDEWEB)

    Chun, Kyung Ah; Lee, Jae Tae; Lee, Sang Woo; Kang, Do Young; Sohn, Snag Kyun; Lee, Jong Kee; Jun, Soo Han; Lee, Kyu Bo [College of Medicine, Kyungpook National Univ., Taegu (Korea, Republic of); Chung, June Key [College of Medicine, Seoul National Univ., Seoul (Korea, Republic of)

    1999-02-01

    To determine whether {sup 99m}Tc-MIBI is recognized by the multidrug resistant P-glycoprotein (Pgp), we have measured quantitatively {sup 99m}Tc-MIBI uptake in cancer cells. The effects of various Pgp reversing agents on cellular {sup 99m}Tc-MIBI uptake were also investigated in the presence of multidrug resistance gene-1 (mdr 1 gene) overexpression. We measured percentage uptake of {sup 99m}Tc-MIBI at different incubation temperatures both in mdr1 positive and negative cells. The effects of verapamil, cyclosporin, and dipyridamole on cellular uptake of {sup 99m}Tc-MIBI were also evaluated with or without overexpression of mdr1 gene in cultured murine leukemia L1210 cells. The mdr1 gene expressing cell lines were effectively induced in in vitro with continuous application of low-dose adriamycin or vincristine. Cellular uptake of {sup 99m}Tc-MIBI was higher in mdr1 negative L1210 cells than those of mdr1 positive cells, and higher when incubated in 37 .deg. C than 4 .deg. C. In the presence of verapamil, cyclosporin or dipyridamole, {sup 99m}Tc-MIBI uptake was increased upto 604% in mdr1 positive cells. Cellular uptake of {sup 99m}Tc-MIBI is lower in leukemia cells over-expressing mdr1 gene, and MDR-reversing agents increase cellular uptake. These results suggest the {sup 99m}Tc-MIBI can be used for characterizing Pgp expression and developing MDR-reversing agents in vitro.

  8. THE VALUE OF 99mTc-MIBI MYOCARDIAL PERFUSION SPECT IMAGING IN DETECTING CORONARY ARTERY DISEASE IN PATIENTS WITH VALVULAR DISEASE BEFORE OPERATION

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective. The aim of this study was to detect coronary artery disease using99m Tc-MIBI myocardial perfusion imaging in patients with valvular disease.Methods. Thirty patients with valvular disease confirmed by echocardiography underwent 99mTc-MIBI myocardial perfusion imaging using multiSPECT 1h after stress test (exercise, dipyridamole or dobutamine test) and were performed coronary angiography within 1 month before valvular operation.Results.For 29 out of the 30 patients, the results of 99mTc-MIBI myocardial perfusion imaging were similar with those of coronary angiography, the concordance rate was 96.7% and the negative predictability was 100%.Conclusion.99m Tc-MIBI myocardial perfusion imaging is a reliable non-invasive method for detecting coronary artery disease in patients with valvular disease and so as to draw up suitable operation programs for them.

  9. Tc-99m MIBI imaging for secondary skeletal involvement in breast and prostate cancers and multiple myeloma

    International Nuclear Information System (INIS)

    Objective:The Objective of the study was to evaluate the role of Tc-99m MIBI whole body imaging in assessing secondary osseous involvement in patients with malignancy of breast, prostate or multiple myeloma. In this study a total of 41 patients were included. Out of these 18 had breast carcinoma, 12 had prostate carcinoma and 11 were diagnosed cases of multiple myeloma. All patients had their whole body MIBI imaging done which was compared with MDP bone scan by employing some other diagnostic modality (plain radiographs, CT scan, MRI scan or histopathological evidence) to confirm the lesions detected by either of the scans. The results of all the studies were evaluated qualitatively by assessing the number of lesions visually by three experienced nuclear physicians. Quantitative analysis of the lesions was also done, by calculating the lesion to normal uptake ratio, to augment the findings of visual assessment and for statistical analysis. Results: Results obtained in this study by MIBI and MDP imaging varied significantly among different groups and subgroups of patients depending on the primary malignancy and stage of therapy. However results obtained by imaging of patients within a group and subgroup were consistent with each other. MIBI scan showed a sensitivity of 99% in cases of multiple myeloma, where MDP scan was only 16% sensitive. In case of pre-therapy patients of breast and prostate carcinoma, the sensitivity of MIBI scan came out to be 80% and 74% respectively. In patients who were on chemo/radiotherapy MIBI scan was 54% sensitive in patients with breast carcinoma and 38% sensitive in patients with prostate carcinoma. MDP scan showed a sensitivity of 100% in all the groups. The positive predictive value of MIBI scan came out to be 100% but that of MDP was 42-76% in different groups. It is therefore concluded that the most significant role of MIBI imaging is in detection of bone metastases secondary to breast and prostate carcinoma in pre

  10. Scintigraphy with Tc99m-MIBI to annual prediction the evolution of women referred for evaluation of precordial pain

    International Nuclear Information System (INIS)

    In order to evaluate the capacity of myocardial perfusion scintigraphy with Tc99m-MIBI to predict the evolution of women referred for evaluation of precordial pain, the rates of cardiovascular events at one year were studied in a group of patients with normal scintigraphy (group 1: 17 patients) and in another with reversible perfusion defects (group 2: 17 patients). The one-day protocol was applied for scintigraphy with Tc99m-MIBI (rest/stress) with combined stress (ergometrin bicycle plus 0.28 mg/Ig of dipyridamole) in those cases where it was necessary. A quantitative and qualitative analysis of the regional uptake was made. Patients from group 1 were younger than those from group 2 (average age: 49 vs 55, respectively, p = 0.04) and they had less hypercholesterolemia (112 % vs 59 %, respectively, p = 0.01). The rates of appearance of cardiovascular events at one year were calculated for patients from group 2 by using the Kaplan-Meier's method. The rates of appearance of clinical events (myocardial infarction and unstable angina) and of revascularization procedures (percutaneous transluminal coronary angioplasty and aortocoronary bypass surgery) were 0.24 in both cases. Only a patient from group 1 presented varying angina at 5 months. No deaths were reported in any of the groups. 94 % of the patients with normal perfusion scintigraphy were free of events after a one-year follow-up, whereas only 53 % of those with reversible perfusion defects showed a similar behavior. It was concluded that myocardial perfusion scintigraphy with Tc99m-MIBI is a useful tool to predict the evolution of women referred for evaluation of precordial pain at one year

  11. 99mTc-MIBI scintigraphy as a functional method for multi drug resistance (MDR) evaluation in breast cancer patients

    International Nuclear Information System (INIS)

    Full text: The purpose of this study was to evaluate the role of 99mTc-MIBI scintigraphy for assessing MDR in breast cancer (BC) patients and correlate the results with P-gp overexpression. 22 women of 35-68 years age having breast cancer were studied. Two or 3 cycles of chemotherapy (NCT) were given to all patients using standard regimen (FEC in 15 and CMF in 7). Planar and SPECT scintigraphy using 740-925 MBq Tc-99m MIBI was performed before and after NCT. Focal uptake of Tc-99m MIBI in BC lesions was used as scintigraphic criteria of abnormality. Tumor/Background uptake index (T/B) was also calculated. Immunohistochemistry was carried out after surgery for P-gp detection in all cases. The degree of expression was evaluated according to the semiquantitative score analysis from 0 to 4. Planar imaging was true positive in 21 patients, false positive in 1 patient and false negative in 1 patient. SPECT imaging was true positive in 22 patients, false positive in 1 patient. SPECT showed additional tumor masses in 3 patients with multi focal BC. Sensitivity was 95% (21/22) and 100% (22/22) respectively for planar and SPECT detection of BC. P-gp expression was positive in 40.8% of patients and negative in 59.2%. Intensive 99mTc-MIBI uptake was seen on the planar images in 21 patients independent of P-gp expression. No significant relationship was seen between T/B Index and P-gp detection. Objectively 2 patients showed complete remissions, 50% tumoral reduction in 1 patient and minimal response (20-35% reduction) in 12 pts. No change in the lesion was noticed in 7 patients. T/B Index was reduced after NCT > 20% in 9 patients. In conclusion, use of SPECT is an important diagnostic approach for identification of deeply located breast cancer lesions and satellite tumor spots in multifocal BC. T/B Index does not correlate with P-gp overexpression. (author)

  12. Breast Scintigraphy using Tc-99m MIBI in Patients with Malignant Breast Mass: The correlation between scintigraphy and histopathology

    International Nuclear Information System (INIS)

    Purpose: In this study, we evaluated the relationship between measurements (10 minutes, 2 hours uptake ratio and washout ratio) acquired in Tc-99m MIBI breast scintigraphy and postoperative histopathologic finding. Materials and Methods: We studied 23 patients of breast malignant tumor (mean age = 50 ∫5.8 years old); 16 cases of invasive ductal carcinoma, 3 cases of metaplastic carcinoma, 2 cases of infiltrative ductal carcinoma and 2 cases of atypical ductal carcinoma. In all patients, Tc-99m MIBI breast Scintigraphy was obtained with measuring uptake and washout ratio. The regions of interests (ROI) were drawn within the central portion of the lesion and the diameters of ROI were about 70% of the diameter of the lesion. The uptake ratio was calculated by counts within the lesion in affecting side dividing by the counts of comparable region of opposite side in 10 minutes and 2 hours after injection. Washout ratio was calculated by subtracting counts at 2 hours from these at 10 minutes and dividing by the number of counts at 10 minutes. Histopathologic findings were graded by size of the mass, Ki-67 index, number of mitosis in 10 high power fields, number of metastatic lymphadenopathy and grade of structural atypism. To compare the measurement of Tc-99m MIBI breast scintigraphy and histopathologic finding, we used Pearson's correlation analysis and P<0.05 was regarded as significant. Results: There was statistically significant positive correlation between 10 minutes uptake ratio and 2 hours uptake ratio (r=0.91, P<0.01). There is positive relationship between 10 minutes, 2 hours uptake ratio and size of the lesion (r=0.48, r=0.31), between washout ratio and Ki-67 index (r=0.28), between washout ratio and size(r=0.29), but without statistical significance. There is negative relationship between 10 minutes, 2 hours uptake ratio and KI-67 index (r=-0.36, r=-0.29), between 10 minutes, 2 hours uptake ratio and number of mitosis (r=-0.30, r=-0.22), 10 minutes, 2 hours

  13. Labelling, biodistribution and compartmental analysis of N-acetylcysteine labelled with Tc-99m. Comparative investigation with with 99m Tc-MIBI in an in vivo tumoral model

    International Nuclear Information System (INIS)

    Labelling and biodistribution studies were done with two different ligands, respectively Methoxy isobutyl isonitrile (MIBI) and N-acetylcysteine (NAC), employing Tc-99m as a tracer. The main objective was to assess the pharmacokinetic properties of the second substance, aiming at its possible application in cancer diagnosis. To this purpose an in vivo investigation was done using healthy and tumor-bearing rats with experimental cancer. Images of tumor-bearing rats registered in a scintillation camera indicated that with 99m Tc-MIBI none of the two selected times was adequate for visualization of the cancer mass. In contrast, 99m Tc-NAC permitted clear identification of the humor, four hours after injection. The results have demonstrated that 99m Tc-NAC is a radiopharmaceutical with affinity for cancer tissue and promising for further investigation concerning imaging diagnosis of tumors. (author)

  14. Molecular imaging with {sup 99m}Tc-MIBI and molecular testing for mutations in differentiating benign from malignant follicular neoplasm: a prospective comparison

    Energy Technology Data Exchange (ETDEWEB)

    Giovanella, L.; Treglia, G.; Ceriani, L. [Oncology Institute of Southern Switzerland, Department of Nuclear Medicine and Thyroid Centre, Bellinzona (Switzerland); Campenni, A. [Policlinico Universitario, Istituto di Medicina Nucleare, Messina (Italy); Verburg, F.A. [RWTH University Hospital Aachen, Department of Nuclear Medicine, Aachen (Germany); Trimboli, P. [Oncology Institute of Southern Switzerland, Department of Nuclear Medicine and Thyroid Centre, Bellinzona (Switzerland); Ospedale Israelitico, Sezione di Endocrinologia e Diabetologia, Roma (Italy); Bongiovanni, M. [Centre Hopitalier Universitaire Vaudouise, Institut de Pathologie, Lausanne (Switzerland)

    2016-06-15

    To compare mutation analysis of cytology specimens and {sup 99m}Tc-MIBI thyroid scintigraphy for differentiating benign from malignant thyroid nodules in patients with a cytological reading of follicular neoplasm. Patients ≥18 years of age with a solitary hypofunctioning thyroid nodule (≥10 mm), normal thyrotropin and calcitonin levels, and a cytological diagnosis of follicular neoplasm were prospectively enrolled. Mutation analysis and {sup 99m}Tc-MIBI scintigraphy were performed and patients were subsequently operated on to confirm or exclude a malignant lesion. Mutations for KRAS, HRAS and NRAS and for BRAF and translocations of PAX8/PPARγ, RET/PTC1 and RET/PTC3 were investigated. Static thyroid scintigraphic images were acquired 10 and 60 min after intravenous injection of 200 MBq of {sup 99m}Tc-MIBI and visually assessed. Additionally, the MIBI washout index was calculated using a semiquantitative method. In our series, 26 % of nodules with a follicular pattern on cytology were malignant with a prevalence of follicular carcinomas. {sup 99m}Tc-MIBI scintigraphy was found to be significantly more accurate (positive likelihood ratio 4.56 for visual assessment and 12.35 for semiquantitative assessment) than mutation analysis (positive likelihood ratio 1.74). A negative {sup 99m}Tc-MIBI scan reliably excluded malignancy. In patients with a thyroid nodule cytologically diagnosed as a follicular proliferation, semiquantitative analysis of {sup 99m}Tc-MIBI scintigraphy should be the preferred method for differentiating benign from malignant nodules. It is superior to molecular testing for the presence of differentiated thyroid cancer-associated mutations in fine-needle aspiration cytology sample material. (orig.)

  15. Using 99mTc-MIBI to Evaluate the Effects of Chemosensitizer on P-glycoprotein in Multidrug-resistant Carcinoma Cells

    Institute of Scientific and Technical Information of China (English)

    ZHANGZhenwei; ZHANGXuemei; WUHua; ZHAOMing; XIANYUZhiqun; ZHOUJian; LAIShiying

    2005-01-01

    Objective: To establish a method to evaluate the effects of chemosensitizer on P-glycoprotein using 99mTc-MIBI, and observe the changes of 99mTc-MIBI uptake kinetics and P-glycoprotein levels after using verapamil in MDR human breast cells MCF-7/Adr. Methods: MDR breast carcinoma cells, MCF-7/Adr, were incubated and different protocols were performed. Protocol I: a chemosensitizer, verapamil (10μmol/L), was added into cell culture medium, while in control group, the same volume of DMEM was given. Cells were harvested after 2 h incubation with 99mTc-MIBI. Protocol Ⅱ: Verapamil (10μmol/L) was added into cell culture medium and incubated for 20 min, 40 min, 60 rain, 80 min, 8 h, 24 h, 48 h and 72 h respectively. Cells were harvested after 2 h incubation with 99mTc-MIBI. The radioactivity of the cells was measured and P-glycoprotein expression levels were determined with immunohistochemical stain. Results: Protocol I: After 2h incubation with verapamil the cellular uptake of 99mTc-MIBI was remarkably higher than control group (t=2.33, P0.05). Protocol

  16. Monitoring of Biological Responses of Tumor Cells after Irradiation with 99mTc-MIBI —— An In Vitro Study

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    To explore the possibility to employ 99mTc-MIBI to monitor biological response of tumor cells after irradiation and to observe the relation between the radiation doses and the uptake levels of 99mTc-MIBI in tumor cells, the cells were irradiated with a single dose of 2 Gy, 10 Gy and 20 Gy respectively. The uptake of 99mTc-MIBI in each dosage group was determined before and 24, 48, 72 h after irradiation respectively. Apoptosis index (AI), plating efficiency (PE) of tumor cells was simultaneously determined. There was a positive correlation between uptake levels of 99mTc-MIBI and AI(r=-0.91, P<0.05). A negative correlation was noted between the uptake levels and PE (r=-0.86, P<0.05). It is suggested that 99mTc-MIBI may be used as a tracer to monitor the change of viability state of tumor cells after being irradiated with different doses.

  17. Impact of the parathyroid scintigraphy with MIBI -{sup 99m}Tc in the detection of an infra-centimetric parathyroid adenoma; Impact de la scintigraphie parathyroidienne au MIBI-99mTc dans la detection d'un adenome parathyroidien infracentimetrique

    Energy Technology Data Exchange (ETDEWEB)

    Ben Ali, K.; Mortazavi-Jhanno, N.; Burg; Rouzet, F.; Lebtahi, R.; Le Guludec, D. [Service de medecine nucleaire, hopital Bichat, AP-HP, Paris, (France); Leghzali, H.; Besse, H.; Stievenart, J.L. [service de medecine nucleaire, hopital Beaujon, AP-HP, Paris, (France)

    2009-05-15

    The objective is to enlighten the contribution of the parathyroid scintigraphy with MIBI-{sup 99m}Tc in the detection of infra-centimetric parathyroid adenomas. Conclusions: the small low intense visible centres with a single time of scintigraphy can correspond to adenomas. The association parathyroid scintigraphy with MIBI-{sup 99m}Tc to a cervical echography contributes to raise the sensitivity in the detection of parathyroid infra-centimetric adenomas. (N.C.)

  18. The role of {sup 99m}Tc-MIBI scintigraphy in the assessment of MDR1 overexpression in patients with musculoskeletal sarcomas: comparison with therapy response

    Energy Technology Data Exchange (ETDEWEB)

    Burak, Z.; Erinc, R.; Oezcan, Z.; Dirlik, A. [Dept. of Nuclear Medicine, Ege University Medical Faculty, Bornova (Turkey); Ersoy, Oe.; Basdemir, G. [Dept. of Pathology, Ege University Medical Faculty, Izmit (Turkey); Moretti, J.-L. [Service de Medecine Nucleaire et Biophysique, Univ. Paris 8 (France); Sabah, D. [Dept. of Orthopaedics, Ege University Medical Faculty, Izmir (Turkey)

    2001-09-01

    The occurrence of multidrug resistance (MDR), which is in part due to the overexpression of P-glycoprotein (Pgp), is a major problem in neoadjuvant therapy of malignant musculoskeletal tumours. The aim of this study was to investigate the role of technetium-99m hexakis-2-methoxyisobutylisonitrile ({sup 99m}Tc-MIBI) scintigraphy for functional imaging of the MDR1 phenotype in patients with musculoskeletal sarcomas. We aimed to compare {sup 99m}Tc-MIBI uptake and washout kinetics with the expression of Pgp and with chemotherapy response. Twenty-five patients (16 males and 9 females, aged between 8 and 65 years) with malignant musculoskeletal tumours were studied. After injection of 555-740 MBq {sup 99m}Tc-MIBI, dynamic flow images of the involved area were obtained for 3 min, and planar images were acquired at 10 min and 1 h. From the dynamic images, a tumour perfusion index (TPI) was obtained using Patlak-Rutland analysis. Tumour to background (T/B) ratios of both early and delayed images and percent wash-out rate (WR%) of {sup 99m}Tc-MIBI were calculated. Immunohistochemical analysis of Pgp was performed on biopsy specimens and the degree of expression was graded according to a semiquantitative scoring system, from 0 to 6. After neoadjuvant therapy, tumour response was assessed by examining the ratio of viable cells and by detecting percent necrosis. Scintigraphic results were compared with Pgp status and therapy response. Irrespective of the Pgp status, all patients showed significant perfusion and {sup 99m}Tc-MIBI uptake in early images. There was not a significant correlation between T/B ratios of early and delayed images and Pgp expression. We observed a positive correlation between WR% and Pgp status (r=0.61, P<0.01), and the wash-out rate of {sup 99m}Tc-MIBI was significantly higher in patients with high Pgp expression than in those with a low Pgp score (33%{+-}9% vs 17%{+-}9%). Therapy response was determined in 21 of 25 patients, and in only 5 of 21 cases

  19. Using Tc-99m MIBI scintimammography to differentiate nodular lesions in breast and detect axillary lymph node metastases from breast cancer

    Institute of Scientific and Technical Information of China (English)

    王景; 吴华; 周健; 胡健

    2003-01-01

    Objective To test the clinical value of using Tc-99m MIBI scintimammography (Tc-99m MIBI SMM) to differentiate nodular lesions in breasts and detect axillary lymph node metastases of breast cancer. Methods The subjects consisted of sixty female patients exhibiting unilateral palpable breast mass. The early and delay static acquisitions were undertaken in all subjects at 10 and 90 min after injection with Tc-99m MIBI. The acquisition included three positions: anterior supine, left lateral prone and right lateral prone. A special device for SMM which was developed by our laboratory was used. The ratio of [(T-B)/(NT-B)] was calculated as radioactivity of mass to that of opposite corresponding tissue, and [(T-B)/(NT-B)]≥1.21 was used as the cut-off for semi-quantitative discrimination of malignancy from benignity in semi-quantitative analysis. The final diagnosis was obtained from histology examination in all subjects. Results Using Tc-99m MIBI SMM to differentiate between nodular lesions in breasts, the diagnostic sensitivity and specificity were 92.9% and 90.6% respectively, the positive predictive value 89.7%, the negative predictive value 93.5%, and the accuracy 91.7%. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy obtained with Tc-99m MIBI SMM for detecting axillary lymph node metastases were 83.3%, 86.1%, 80%, 88.6% and 85% respectively. Conclusions It is suggested that Tc-99m MIBI SMM may be not only valuable in differentiating malignant lesions from benign nodular masses of breast, but also helpful in detecting axillary lymph node metastases of breast cancer.

  20. Comparison of 99mTc-MIBI scanning and sentinel node biopsy in the detection of occult melanoma lymph node metastases

    International Nuclear Information System (INIS)

    Aim: Sentinel node biopsy (SNB) is a highly accurate technique for detecting occult metastatic disease in the regional lymph nodes. Our group has reported that 99mTc-MIBI scintigraphy is an imaging technique that can detect clinically undetectable metastases. This prospective study shows preliminary results on the comparison of both techniques for the detection of lymph node invasion. Material and Methods: Twenty-two consecutive patients (15 women, 7 men; mean age, 55 years) with primary melanoma > 1.0 mm thick were enrolled (mean 2.6 mm). Patients underwent 99mTc-MIBI scintigraphy of regional lymph nodes 1-2 weeks before surgery, acquiring 10-minute planar images with a LFOV gamma camera. A preoperative lymphoscintigraphy using 99mTc-colloidal (Re) sulphide was performed the day before surgery, using a dose of 74-93 MBq. Radio guided SNB was performed with a hand-held gamma probe. Lymph nodes were examined histologically and immunostained for S-100 and HMB-45. Results: The sentinel node (SN) was identified in 21/22 patients. An average of 1.6 SN/lesion were harvested from melanomas located in the following regions: head and neck (n=3), trunk (n=6), extremities (n=12). All patients with negative SN (n=11) were also negative with MIBI. In 10 cases the SN demonstrated metastatic involvement, whereas 99mTc-MIBI was positive in 8 of them (80%). In cases with positive SN/negative 99mTc-MIBI, the pathology report revealed micro metastatic disease. Conclusion: These preliminary results suggest that 99mTc-MIBI scanning has the potential of selecting a group of patients who might benefit from a selective complete lymphadenectomy

  1. Diagnostic capabilities of I-131, TI-201, and Tc-99m-MIBI scintigraphy for metastatic differentiated thyroid carcinoma after total thyroidectomy.

    Directory of Open Access Journals (Sweden)

    Fujie,Shunji

    2005-06-01

    Full Text Available

    We investigated the diagnostic capabilities of I-131, Tl-201, and Tc-99m-MIBI (hexakis-2-methoxyisobutyl- isonitrile scintigraphy for thyroid cancer metastases after total thyroidectomy over the entire body and for every locus before and after thyroid bed ablation. After total thyroidectomy of thyroid cancer, 36 cases were subjected to I-131 treatment 64 times. They consisted of 17 men and 19 women with 31 papillary carcinomas and 5 follicular carcinomas. Their ages were 22--75(an average of 60.5+/-12.3 years. I-131 scintigraphy(I-131, Tl-201 scintigraphy(Tl-201, and Tc-99m- MIBI scintigraphy (Tc-99m-MIBI were performed. We defined the metastases as those cases in which serum thyroglobulin (Tgincreased significantly or in which we were able to prove the lesions on CT (computed tomography, MRI (magnetic resonance imaging or bone scintigram. Three radiology medical specialists visually evaluated each scintigram and calculated the sensitivity, specificity, and likelihood ratio. For whole-body sensitivity, both Tl-201 and Tc-99m-MIBI were high before ablation and I-131 was high after ablation. Before ablation, the negative likelihood ratio was less than 0.1 for Tl-201 and Tc-99m-MIBI, while the positive likelihood ratio was more than 10 for Tl-201. After ablation, the positive likelihood ratio for I-131, Tl-201, and Tc-99m-MIBI was more than 10. The sensitivity of the mediastinum was appropriate, except for I-131 before ablation, and the sensitivity of the lung before and after ablation was inferior for either tracer. The specificity of the cervix for I-131 before ablation was markedly deteriorated, but it increased after ablation.

  2. {sup 99m}Tc-MIBI SPECT in primary hyperparathyroidism. Influence of concomitant vitamin D deficiency for visualization of parathyroid adenomas

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, M.; Thoma, N.; Dietlein, M.; Moka, D.; Eschner, W.; Schicha, H. [Dept. of Nuclear Medicine, Univ. of Cologne (Germany); Faust, M. [Dept. of II of Internal Medicine (Endocrinology), Univ. of Cologne (Germany); Schroeder, W. [Dept. of Surgery, Univ. of Cologne (Germany); Huelst-Schlabrendorff, M. von; Ehses, W. [Dept. of Endocrinology Clinic, Univ. of Cologne (Germany)

    2008-07-01

    Aim of the study was to analyse the influence of a concomitant vitamin D deficiency on the results of {sup 99m}Tc-MIBI studies in patients (pts) with primary hyperparathyroidism (pHPT). Patients, methods: between January 1998 and May 2004, 71 pts with pHPT had undergone operation after a {sup 99m}Tc-MIBI study of whom 54 pts (76%) had normal values of 25-OH-vitamin D3 and 17 pts (24%) had vitamin D deficiency. Results of a dual-phase {sup 99m}Tc-MIBI protocol with SPECT were compared with histopathology. Results: in 54 pts with normal vitamin D values late SPECT images identified more lesions (n = 51, sensitivity 91%) than early planar (n = 45, sensitivity 82%) or late planar images (n = 50, sensitivity 88%). In 17 pts with vitamin D deficiency late SPECT images identified more lesions (n = 13, sensitivity 72%) than early planar (n = 10, sensitivity 56%) or late planar images (n = 10, sensitivity 56%) too. In pts with vitamin D deficiency the sensitivity of a {sup 99m}Tc-MIBI SPECT study was lower than in those with normal vitamin D status (72% vs. 91%) and dependent on the value for PTH. However, the results did not reach statistical significance: early planar: p = 0.1625; late planar: p = 0.0039; {sup 99m}Tc-MIBI SPECT: p = 0.1180. Conclusion: the likelihood of a pathological {sup 99m}Tc-MIBI study being obtained in pts with pHPT is dependent on the parathyroid hormone level. However, a negative influence of a low vitamin D level on the scintigraphic detection rate of a parathyroid adenoma could not be proven which may be due to the low number of pts with vitamin D deficiency. (orig.)

  3. Magnetic resonance Imaging (MRI) and technetium-99m-methoxyisonitrile (MIBI) scintigraphy to evaluate the abnormal parathyroid gland and PEIT efficacy for secondary hyperparathyroidism

    Energy Technology Data Exchange (ETDEWEB)

    Wada, Akihiko; Sugihara, Masaki; Sugimura, Kazuro [Shimane Medical Univ., Izumo (Japan); Kuroda, Hiroyuki

    1999-08-01

    Percutaneous ethanol injection therapy (PEIT) of the abnormal parathyroid gland is an effective treatment in patients with chronic renal failure with dialysis that tends to be unresponsive to medication. To evaluate the efficacy of PEIT, we investigated the correlation between serum intact PTH (iPTH), and the findings of MR imaging and {sup 99m}Tc-MIBI scintigraphy. PEIT was performed 32 times in 24 patients with secondary hyperparathyroidism. Both MR imaging and MIBI scintigraphy were performed before and after PEIT. The detectability of parathyroid lesions was evaluated by MRI and MIBI scintigraphy with reference to ultrasound (B-mode) findings (as a standard) and the comparison of each treatment was done between imaging changes and serum iPTH levels. In the small parathyroid glands (<0.5 ml with US), MR images detected abnormal glands in 74.0% (20/27), and MIBI accumulation was observed in 40.7% of the cases. Whereas, MR images and MIBI showed similar detectability (95.0% vs. 90.0%) to large parathyroid glands ({>=}0.5 ml with US). After PEIT, glands in which ethanol was successfully injected showed decreased signal intensity on T2-weighted images and decreased accumulation of MIBI. MR imaging and MIBI scintigraphy showed almost equivalent high sensitivity in evaluating therapeutic changes (73.3% vs. 76.4%) according to our evaluation category of more than 30% reduction of iPTH as effective. However, the specificity of MR imaging was much higher (100%) than that of MIBI scintigraphy in evaluating therapeutic effects. In conclusion, MRI provided better detectability of abnormal parathyroid glands than {sup 99m}Tc-MIBI scintigraphy. The reduction of high signal intensity area after PEIT on T2-weighted MR images is considered an useful therapeutic evaluation guideline than the reduction of MIBI accumulation on scintigraphy. (author)

  4. Comparison of thallium-201, Tc-99m MIBI and I-131 scan in the follow-up assessment after I-131 ablative therapy in differentiated thyroid cancer

    Energy Technology Data Exchange (ETDEWEB)

    Kwon, Jae Sung; Lee, Sung Keun; Kim, Doe Min; Park, Sae Jong; Jang, Kyong Sun; Kim, Eun Sil; Kim, Chong Soon [Hanil General Hospital, Seoul (Korea, Republic of)

    1999-08-01

    We conducted a comparative study to evaluate the diagnostic values of Tl-201, Tc-99m MIBI and I-131 scans in the follow-up assessment after ablative I-131 therapy in differentiated thyroid cancer. The study population consisted of 20 patients who underwent surgical removal of thyroid cancer and ablative radioactive iodine therapy, and followed by one or more times of I-131 retreatment (33 cases). In all patients, Tl-201, Tc-99m MIBI, diagnostic and therapeutic I-131 scans were performed and the results were analyzed retrospectively. Also serum thyroglobulin levels were measured in all patients. The final diagnosis of recurrent or metastatic thyroid cancer was determined by clinical, biochemical, radiologic and/or biopsy findings. Positive rates (PR) of Tc-99m MIBI, Tl-201, diagnostic and therapeutic I-131 scans in detecting malignant thyroid tissue lesions were 70% (19/27), 54% (15/28), 35% (17/48) and 63% (30/48), respectively. The PR in the group of 20 cases (28 lesions) who underwent concomitant Tl-201 and I-131 scans were in the order of therapeutic 131 scan 71%, Tl-201 scan 54% and diagnostic I-131 scan 36%. There was no statistically significant difference between Tl-201 and diagnostic I-131 scans (p>0.05). In the group of 20 cases (27 lesions) who underwent concomitant Tc-99m MIBI and I-131 scans, the PR were in the order of Tc-99m MIBI scan 70%, I-131 therapeutic scan 52% and I-131 diagnostic scan 33%. The PR of Tc-99m MIBI was significantly higher than that of diagnostic I-131 scan (p<0.05). Tc-99m MIBI scan is superior to diagnostic I-131 scan in detecting recurrent or metastatic thyroid cancer following ablation therapy in patients with differentiated thyroid cancer. Tl-201 scan did not showed significantly higher positive rate than diagnostic I-131 scan. Instead of diagnostic I-131 scan before the I-131 retreatment, Tc-99m MIBI scan without discontinuing thyroid hormone replacement would be a prudent and effective approach in the management of these

  5. Role of pre-operative imaging using {sup 99m}Tc-MIBI and neck ultrasound in patients with secondary hyperparathyroidism who are candidates for subtotal parathyroidectomy

    Energy Technology Data Exchange (ETDEWEB)

    Fuster, David; Ortin, Jaime; Setoain, Xavier; Paredes, Pilar; Duch, Joan; Pons, Francesca [Hospital Clinic, Nuclear Medicine Department, Barcelona (Spain); Ybarra, Juan; Torregrosa, Jose-Vicente [Hospital Clinic, Renal Transplant Unit, Barcelona (Spain); Gilabert, Rosa [Hospital Clinic, Radiology Department, Barcelona (Spain)

    2006-04-15

    The purpose of this study was to assess whether pre-operative {sup 99m}Tc-methoxyisobutylisonitrile (MIBI) scintigraphy and neck ultrasound (US) are of value in improving the outcome of subtotal parathyroidectomy in patients with secondary hyperparathyroidism. Forty-eight consecutive haemodialysis patients with severe secondary hyperparathyroidism prospectively underwent ''blinded'' subtotal parathyroid surgery, with 1-year follow-up to establish cure or relapse of their secondary hyperparathyroidism. Double-phase {sup 99m}Tc-MIBI scintigraphy and neck US were performed pre-operatively in all patients. When the preserved gland showed {sup 99m}Tc-MIBI uptake or an abnormal size on US, it was considered that ''{sup 99m}Tc-MIBI advice'' and ''US advice'', respectively, had not been followed. Pre-operative and follow-up parathyroid hormone (PTH) levels were obtained in all patients. All data were evaluated on a patient by patient basis. Four parathyroid glands were identified in each patient at primary surgery, resulting in an operative success rate of 100%. Their weight ranged from 15 to 7,300 mg (mean 1,120{+-}900 mg). Nine of the 48 patients (19%) showed a recurrence of their secondary hyperparathyroidism. The recurrence rate was 2% (1/48) and 10% (5/48), respectively, when {sup 99m}Tc-MIBI and US advice was followed. The sensitivity, specificity, NPV and PPV for pre-operative imaging were 72%, 95%, 97% and 80% respectively for {sup 99m}Tc-MIBI, and 55%, 67%, 87% and 28% for US. {sup 99m}Tc-MIBI scintigraphy is a reliable non-invasive exploratory tool and its preoperative use results in a significant reduction in the number of recurrences in haemodialysis patients with secondary hyperparathyroidism who are candidates for subtotal parathyroidectomy. The use of neck US did not significantly improve the results obtained with {sup 99m}Tc-MIBI alone. (orig.)

  6. Evaluation of MS-209, a novel multidrug-resistance-reversing agent, in tumour-bearing mice by technetium-99m-MIBI imaging

    Energy Technology Data Exchange (ETDEWEB)

    Tatsumi, Mitsuaki; Nishimura, Tsunehiko [Division of Tracer Kinetics, Osaka University Graduate School of Medicine (Japan); Tsuruo, Takashi [Department of Chemical Biology, Institute of Molecular and Cellular Biosciences, University of Tokyo (Japan)

    2002-03-01

    MS-209 is a newly synthesised multidrug-resistance (MDR)-reversing agent with few side-effects. In this study, we evaluated the effect of MS-209 on P-glycoprotein (Pgp)-positive tumours in mice by means of technetium-99m methoxyisobutylisonitrile (MIBI) imaging. Mice received Pgp-negative KB-3-1 or Pgp-positive KB-C1 cell xenografts. KB-3-1-bearing mice were administered 0 or 100 mg/kg of MS-209 (groups S0 and S100, respectively), and KB-C1-bearing mice 0, 50, 100 or 200 mg/kg (groups R0, R50, R100 and R200, respectively), 30 min before imaging studies. Dynamic {sup 99m}Tc-MIBI images were acquired for 30 min, followed by biodistribution studies. Washout of {sup 99m}Tc-MIBI from the tumours was faster in group R0 than in group S0 on visual analysis, and the washout half-time estimated by region of interest analysis was shorter in group R0 than in group S0. Biodistribution studies revealed that {sup 99m}Tc-MIBI accumulation in tumours (expressed as %ID/g) was also lower in group R0 than in group S0. MS-209 delayed the {sup 99m}Tc-MIBI washout from KB-C1 tumours. Washout half-time was longer in groups R50, R100 and R200 than in group R0. A higher %ID/g was observed in groups R100 and R200 than in group R0. In KB-3-1 tumours, MS-209 had no effect on the washout half-time or the %ID/g. This study demonstrated that MS-209 increases the {sup 99m}Tc-MIBI accumulation in Pgp-positive tumours. The MDR-reversing effect of MS-209 could be effectively evaluated with {sup 99m}Tc-MIBI visually and non-invasively in vivo. {sup 99m}Tc-MIBI imaging with MS-209 prior to chemotherapy should contribute significantly to the treatment strategy in patients with multidrug-resistant tumours. (orig.)

  7. Frequency and severity of myocardial perfusion abnormalities using Tc-99m MIBI SPECT in cardiac syndrome X

    International Nuclear Information System (INIS)

    Cardiac syndrome X is defined by a typical angina pectoris with normal or near normal (stenosis <40%) coronary angiogram with or without electrocardiogram (ECG) change or atypical angina pectoris with normal or near normal coronary angiogram plus a positive none-invasive test (exercise tolerance test or myocardial perfusion scan) with or without ECG change. Studies with myocardial perfusion imaging on this syndrome have indicated some abnormal perfusion scan. We evaluated the role of myocardial perfusion imaging (MPI) and also the severity and extent of perfusion abnormality using Tc-99m MIBI Single Photon Emission Computed Tomography (SPECT) in these patients. The study group consisted of 36 patients with cardiac syndrome X. The semiquantitative perfusion analysis was performed using exercise Tc-99m MIBI SPECT. The MPI results were analyzed by the number, location and severity of perfusion defects. Abnormal perfusion defects were detected in 13 (36.10%) cases, while the remaining 23 (63.90%) had normal cardiac imaging. Five of 13 (38.4%) abnormal studies showed multiple perfusion defects. The defects were localized in the apex in 3, apical segments in 4, midventricular segments in 12 and basal segments in 6 cases. Fourteen (56%) of all abnormal segments revealed mild, 7(28%) moderate and 4 (16%) severe reduction of tracer uptake. No fixed defects were identified. The vessel territories were approximately the same in all subjects. The Exercise treadmill test (ETT) was positive in 25(69%) and negative in 11(30%) patients. There was no consistent pattern as related to the extent of MPI defects or exercise test results. Our study suggests that multiple perfusion abnormalities with different levels of severity are common in cardiac syndrome X, with more than 30 % of these patients having at least one abnormal perfusion segment. Our findings suggest that in these patients microvascular angina is probably more common than is generally believed

  8. The key role of 99mTc-MIBI SPECT/CT in the diagnosis of parathyroid adenoma: a case report.

    Science.gov (United States)

    Li, Qing; Pan, Jiemin; Luo, Quanyong; Wang, Yan; Bao, Yuqian; Jia, Weiping

    2015-06-01

    The concomitant occurrence of primary hyperparathyroidism (PHPT) due to parathyroid adenoma and papillary thyroid carcinoma (PTC) is not common. The co-occurrence of parathyroid tumors and thyroid diseases can lead to misdiagnosis owing to mutual interference of imaging in the early period of disease. The most commonly used imaging techniques for the preoperative localization of parathyroid and thyroid adenomas are technetium-99m sestamibi (99mTc-MIBI) scintigraphy and ultrasonography of the neck. Recently, 99mTc-MIBI single-photon emission computed tomography associated with computed tomography scintigraphy (SPECT/CT) has been used to detect PHPT, and its diagnostic value is being evaluated. Herein, we report a patient with an unusual co-occurrence of parathyroid adenoma and multiple thyroid diseases, including papillary thyroid carcinoma, thyroid adenoma and lymphocytic thyroiditis, which were localized with 99mTc-MIBI SPECT/CT and confirmed surgically. We suggest that 99mTc-MIBI SPECT/CT can play an important role in the diagnosis of parathyroid and thyroid lesions. PMID:26154096

  9. The key role of {sup 99m}Tc-MIBI SPECT/CT in the diagnosis of parathyroid adenoma: a case report

    Energy Technology Data Exchange (ETDEWEB)

    Li, Qing; Pan, Jiemin; Luo, Quanyong; Wang, Yan; Bao, Yuqian; Jia, Weiping, E-mail: wpjia@sjtu.edu.cn, E-mail: wpjia@yahoo.com [Shanghai Jiao Tong University Affiliated Sixth People' s Hospital, Shanghai (China)

    2015-06-15

    The concomitant occurrence of primary hyperparathyroidism (PHPT) due to parathyroid adenoma and papillary thyroid carcinoma (PTC) is not common. The co-occurrence of parathyroid tumors and thyroid diseases can lead to misdiagnosis owing to mutual interference of imaging in the early period of disease. The most commonly used imaging techniques for the preoperative localization of parathyroid and thyroid adenomas are technetium-99m sestamibi ({sup 99m}Tc-MIBI) scintigraphy and ultrasonography of the neck. Recently, {sup 99m}Tc-MIBI single-photon emission computed tomography associated with computed tomography scintigraphy (SPECT/CT) has been used to detect PHPT, and its diagnostic value is being evaluated. Herein, we report a patient with an unusual co-occurrence of parathyroid adenoma and multiple thyroid diseases, including papillary thyroid carcinoma, thyroid adenoma and lymphocytic thyroiditis, which were localized with {sup 99}mTc-MIBI SPECT/CT and confirmed surgically. We suggest that {sup 99m}Tc-MIBI SPECT/CT can play an important role in the diagnosis of parathyroid and thyroid lesions. (author)

  10. The key role of 99mTc-MIBI SPECT/CT in the diagnosis of parathyroid adenoma: a case report

    International Nuclear Information System (INIS)

    The concomitant occurrence of primary hyperparathyroidism (PHPT) due to parathyroid adenoma and papillary thyroid carcinoma (PTC) is not common. The co-occurrence of parathyroid tumors and thyroid diseases can lead to misdiagnosis owing to mutual interference of imaging in the early period of disease. The most commonly used imaging techniques for the preoperative localization of parathyroid and thyroid adenomas are technetium-99m sestamibi (99mTc-MIBI) scintigraphy and ultrasonography of the neck. Recently, 99mTc-MIBI single-photon emission computed tomography associated with computed tomography scintigraphy (SPECT/CT) has been used to detect PHPT, and its diagnostic value is being evaluated. Herein, we report a patient with an unusual co-occurrence of parathyroid adenoma and multiple thyroid diseases, including papillary thyroid carcinoma, thyroid adenoma and lymphocytic thyroiditis, which were localized with 99mTc-MIBI SPECT/CT and confirmed surgically. We suggest that 99mTc-MIBI SPECT/CT can play an important role in the diagnosis of parathyroid and thyroid lesions. (author)

  11. Preparation and evaluation of modified composition for lyophilized kits of [Cu(MIBI4]BF4 for [99mTc] technetium labeling

    Directory of Open Access Journals (Sweden)

    Marisa de Jesus de C. Lima

    2005-10-01

    Full Text Available The [Cu(MIBI4]BF4 complex was synthesized and different formulations for lyophilized kits that could be cost-effectively used with different routines in nuclear medicine laboratories were investigated. In one preparation the kit components were kept similar to the Cardiolite® , except that the SnCl2.2H2O concentration was increased to 0.150 mg. In a second formulation, component concentrations were reduced to 1/5 of the original value and the SnCl2.2H2O concentration was adjusted to 0.04 mg. These products were labeled with maximum activities of 55.5 GBq and 8.14 GBq, respectively, and have shown an average radiochemical purity of 95 %. Biodistribution of the products was assessed by dissection in mice and in rabbits, and did not show any statistical difference when compared to Cardiolite®. In the synthesis of [Cu(MIBI4]BF4 a new procedure was introduced for the synthesis of N-(2-methyl-propenyl-formamide, with the use of microwave radiation as heat source. This modification reduced the reaction time to 25 seconds, while maintaining a yield of 68%.Neste trabalho são apresentados dados relativos a síntese do complexo [Cu(MIBI4]BF4 e à utilização do mesmo para produção de kit liofilizado para obtenção do radiofármaco [[99mTc](MIBI6]+. No processo de síntese foi utilizado radiação de microondas como fonte de aquecimento em uma etapa do processo, permitindo que a reação fosse executada em 25 segundos, obtendo rendimento de 68 %. Na preparação dos kits liofilizados, um deles denominado FULL, nos quais a quantidade dos componentes foi mantida igual ao produto comercial Cardiolite®, e outro denominado FRACTION, nos quais as quantidades foram reduzidas a 1/5 do total, foram adicionadas quantidades extras de SnCl2.2H2O. Deste modo, tornou-se possível marcar os kits liofilizados com atividades máximas de 55,5 GBq e 8,14 GBq, respectivamente. A biodistribuição dos produtos marcados, em camundongos e coelhos, não mostrou diferen

  12. Are there any differences in uptake of Tc-99m MIBI and tetrofosmin between cancer cells expressing P-glycoprotein and MDR-related protein?

    Energy Technology Data Exchange (ETDEWEB)

    Seo, J. H.; Yoo, J. A.; Seo, M. R.; Bae, J. H.; Jeong, S. Y.; Ahn, B. C.; Lee, K. B.; Lee, J [Kyungpook National University Hospital, Daegu (Korea, Republic of)

    2004-07-01

    Uptake of Tc-99m MIBI and Tc-99m tetrofosmin(TF) in two multidrug resistance cells, human colon cancer HCT/CLO2 cells expressing P-glycoprotein(Pgp) and non-small cell lung cancer A549 cells showing multidrug-resistance related protein(MRP) in vivo. RT-PCR Western blot analysis and immunohistochemistry(IHC) were used to detect Pgp and MRP. Cellular uptake of MIBI and TF was measured with single cell suspension of HCR/CLO2 and 549 cells. Cells were inoculated in flanks of 24 nude mice. Group 1 (Gr1) and Gr3 mice were injected with only MIBI or TF, and Gr2 and Gr4 mice were treated with cyclosporin A(CsA) before injection of MIBI or TF. Mice were sacrificed at 10, 60 and 240-min after tracer injection. Radioactivities of organs and tumors were measured. 549 cells expressed MRPr1 protein detected by Western blot and IHC staining of tumor tissue at cell membrane but not Pgp, A549/CLO2 was vice versa. Cellular uptake and % increases with CsA were higher for MIBI than TF for both cells in vitro. Animal studies showed that MIBI and TF uptake were progressively increased by time in both Pgp(+) HCT/CLO2 tumor and MRP(+) A549 cells in both Gr1 and Gr3. There were no significant differences in tumoral uptake of MIBI and TF between Grl and Gr3 for both cells except higher MIBI uptake in Pgp cells at 60-min. % increases of MIBI (111% at 10 min, 220% at 60 min, 426% at 240 min) and TF uptake (186% at 10 min 579% at 60 min, 390% at 240 min) were progressively increased with time up to 240-min with CsA % increases of MIBI (114% at 10 min, 257% at 60 min 396% at 240 min) and TF uptake (110% at 10 min, 205% at 60 min, 410% at 240 min) were similar with those in HCT/CLO2 tumor. There were no differences in MIBI and TF uptake between Pgp and MRP tumors. Although MIBI showed better MDR reversal effect of modulator CsA than TF in vitro, there were no differences for both tumors in vivo. MIBI and TF are suitable tracers for imaging Pgp and MRP-mediated drug resistance in cancers.

  13. Diagnostic accuracy of quantitative 99mTc-MIBI scintimammography according to ROC curve analysis

    Energy Technology Data Exchange (ETDEWEB)

    Kim, J. H.; Lee, H. K.; Seo, J. W.; Cho, N. S.; Cha, K. H.; Lee, T. H. [Gachon Medical College, Gil Medical Center, Inchon (Korea, Republic of)

    1998-07-01

    99mTc-sestamibi scintimammography (SMM) has been shown to be a useful diagnostic test in the detection of breast cancer and the receiver operating characteristic (ROC) curve analysis provides detailed information of a diagnostic test. The purpose of this study was to evaluate the feasibility and efficacy of quantitative indices of SMM in the detection of malignant breast lesions according to ROC analysis. Prone anterior, lateral planar and supine SPECT imagings were performed on 75 female patients (mean age=43.4 yr) with breast mass (size{>=}0.8cm) after intravenous injection of 20-30 mCi 99mTc-sestamibi. 45 Malignant (Invasive ductal ca(36), Inv lobular ca(5), Inv duc + lob (1), Inv tubular ca (3)) and 30 benign (fibroadenoma (13), fib cyst(12), Fat necrosis(3), papilloma(1), paraffinoma (1)) lesions were histologically proven. Data were analyzed by creating three regions of interest (ROIs) over designated areas: lesion, normal breast and right chest wall. Lesion to normal (L/NL) and lesion to chest wall (L/CW) ratios were calculated for each patient both on the planar and SPECT. The area under the ROC curve (AUC) was calculated and compared among four semiquantitative indices and an average scintimammographic index (SMM(mean)) from arithmatic mean. ROC curve analysis revealed planar L/N, SPECT L/N and L/CW ratios provide comparable better diagnostic accuracies for detection of breast cancer than planar L/CW ratio (p<0.05), respectively. For quantitative SMM of 75 lesions, malignancy rate was 60%, and Sensitivity, Specificity, Positive Predictive Value, Negative Predictive Value and Accuracy were 0.78, 0.77, 0.84, 0.72 and 0.77, respectively. Quantitative SMM is an useful objective method for differentiating malignant from benign breast lesions.

  14. Evaluation of parathyroid imaging methods with {sup 99m}Tc-MIBI. The comparison of planar images obtained using a pinhole collimator and a parallel-hole collimator

    Energy Technology Data Exchange (ETDEWEB)

    Fujii, Hirofumi; Iwasaki, Ryuichiro; Hashimoto, Jun; Nakamura, Kayoko; Kunieda, Etsuo; Sanmiya, Toshikazu; Kubo, Atsushi [Keio Univ., Tokyo (Japan). School of Medicine; Ogawa, Koichi; Inagaki, Kazutoshi

    1999-07-01

    Parathyroid scintigraphy with {sup 99m}Tc-MIBI was performed using two kinds of collimators, namely, a pinhole one and a parallel-hole one, to evaluate which one was more suitable for the detection of hyperfunctioning parathyroid lesions. In the studies using {sup 99m}Tc source, the pinhole collimator showed better efficiency and spatial resolution in the distance where the parathyroid scan are actually performed. In the phantom study, the nodular activities modeling parathyroid lesions were visualized better on the images obtained using the pinhole collimator. In clinical studies for 30 patients suspicious of hyperparathyroidism, hyperfunctioning parathyroid nodules were better detected when the pinhole collimator was used. In conclusion, the pinhole collimator was thought to be more suitable for parathyroid scintigraphy with {sup 99m}Tc-MIBI than the parallel-hole collimator. (author)

  15. Diffuse Bone Marrow Uptake of {sup 99m}Tc-MIBI in A Case of Intravascular Large B-cell Lymphoma

    Energy Technology Data Exchange (ETDEWEB)

    Moon, Seung Hwan; Oh, So Won; Paeng, Jin Chul; Paik, Jin Ho; Lee, Dong Soo [Seoul National University College of Medicine, Seoul (Korea, Republic of)

    2009-08-15

    Intravascular large B-cell lymphoma (IVLBCL) is a subtype of diffuse large cell lymphoma, characterized by proliferation of lymphoid cells in the intravascular space of various organs without causing a mass effect. Although {sup 18}F-FDG PET is a powerful imaging tool in lymphoma, the usefulness of {sup 18}F-FDG PET in the assessment of IVLBCL is still controversial. {sup 99m}Tc-MIBI, a tumor imaging radiopharmaceutical with a different mechanism from that of {sup 18}F-FDG, has been reported to be also effective in lymphoma. However, there is nearly no report on the efficacy of {sup 99m}Tc-Mibg in the assessment of IVLBCL. We present one case of IVLBCL that showed {sup 99m}Tc-MIBI accumulation in the involved bone marrow as an incidental finding, which was discrepant from that of {sup 18}F-FDG PET.

  16. Prediction of response to chemotherapy in locally advanced breast cancer patients using Tc-99m MIBI scintimammography

    International Nuclear Information System (INIS)

    Locally advanced breast cancer is a common presentation in our part of the world. Down staging of the disease followed by surgery and further therapy has shown to prolong survival in such patients. Early prediction of response to therapy and chemo-resistance is of vital importance to ensure better outcome. The study was done to evaluate the possible role of Tc-99m MIBI scintimammography (MSM) in reliable and early prediction of response to therapy through tracer dynamics of the tumor. Response assessment through clinical criteria and other available modalities was also compared. 16 female patients were evaluated through dynamic and static prone MSM performed before and after two cycles of chemotherapy. Decay corrected washout curves were generated by drawing regions of interest (ROIs) all around the tumor. The tracer washout kinetics of the tumor were studied through half life estimation and tracer retention at 60 minute with reference to peak activity on pre and post therapy studies. Results were subjected to statistical analysis. There was a good correlation between the clinical assessment and dynamic MSM findings in good responders while the dynamic MSM was able to provide better information in poor responders. The results conclude that using tracer kinetics of MSM, early and reliable prediction of response to therapy and chemo-resistance may be possible

  17. Scintimammography with {sup 99m}Tc-MIBI versus dynamic MRI for non-invasive characterization of breast masses

    Energy Technology Data Exchange (ETDEWEB)

    Imbriaco, M.; Del Vecchio, S.; Pace, L. [Nuclear Medicine Center of the National Research Council (CNR), University Napoli (Italy); Riccardi, A.; Di Salle, F.; Di Gennaro, F.; Salvatore, M.; Sodano, A. [Department of Biomorphological and Functional Sciences, University ' ' Federico II' ' , Napoli (Italy)

    2001-01-01

    Although mammography remains the technique of choice for the early detection of breast cancer, several imaging techniques, including scintimammography and magnetic resonance imaging (MRI), have recently been proposed as adjuncts for this purpose and included in many diagnostic protocols. This study was undertaken to assess the clinical accuracy of scintimammography with technetium-99m methoxyisobutylisonitrile (MIBI) and contrast-enhanced MRI in the detection of primary breast carcinoma in patients with equivocal mammographic findings. Forty-nine patients with a suspicious breast mass detected either by physical examination or by mammography and ultrasound (US) were studied. All patients underwent scintimammography and dynamic contrast-enhanced MRI 1 week apart. The results of the two techniques were compared and correlated to the final diagnoses. Two independent readers reported the scans using a four-point confidence scale. The areas under the receiver operator characteristic (ROC) curves were obtained. Scintimammography showed an accuracy for tumour detection of 84%, with a sensitivity of 80% and a specificity of 88%. MRI showed an accuracy of 86%, with a sensitivity and specificity of 96% and 75%, respectively. Comparison of the two areas under the ROC curves showed no significant differences between MRI, 0.91{+-}0.05 (mean{+-}SD), and scintimammography, 0.88{+-}0.05 (P=0.9). It is concluded that dynamic MRI and scintimammography possess comparable accuracy in the diagnosis of primary breast carcinoma in patients with equivocal mammographic or US findings. (orig.)

  18. Thyroid nodules with indeterminate cytology: molecular imaging with 99mTc-methoxyisobutylisonitrile (MIBI) is more cost-effective than the Afirma registered gene expression classifier

    International Nuclear Information System (INIS)

    To compare the cost-effectiveness of 99mTc-methoxyisobutylisonitrile (MIBI) thyroid scintigraphy and the Afirma registered gene expression classifier for the assessment of cytologically indeterminate thyroid nodules. A decision tree model was used. Costs were calculated from the perspective of the German health insurance system. The robustness of the results was assessed with probabilistic sensitivity analyses using a Monte Carlo simulation. Life expectancy was 34.3 years (estimated costs per patient EUR1,459 - EUR2,224) for the MIBI scan and 34.1 years (estimated costs EUR3,560 - EUR4,071) for the molecular test. These results were confirmed by the Monte Carlo simulation. MIBI thyroid scintigraphy is more cost-effective than the gene expression classifier. (orig.)

  19. Relationship Between Adenosine - Induced ST Segment Depression During 99mTc-MIBI Scintigraphy and The Severity of Coronary Artery Disease

    International Nuclear Information System (INIS)

    Pharmacologic coronary vasodilation in conjunction with myocardial perfusion scintigraphy has become an alternative to dynamic exercise test for the diagnosis and risk stratification of coronary artery disease, especially in patients who are unable to perform adequate exercise. Dipyridamole and adenosine have been used for pharmacologic stress testing with myocardial perfusion imaging. Adenosine is a potent, coronary vasodilator with rapid onset of action, short half life, near maximal coronary vasodilation and less serious side effects. ST segment depression has been reported in about 7-15% of patients with coronary artery disease receiving dipyridamole in conjunction with myocardial perfusion imaging. The exact cause and clinical significance are not known. In order to evaluate the relationship between adenosine-induced ST segment depression during 99mTc-MIBI myocardial perfusion scintigraphy and the severity of coronary artery disease, we performed 99m-MIBI imaging after intravenous infusion of adenosine in 120 patients with suspected coronary artery disease. Of the 120 patients, 28 also performed coronary angiography. There were 24 patients with ST segment depression during 99mTc-MIIBI scintigraphy and 96 patients without ST segment depression. Adenosine was infused intravenously at a dose of 0.14 mg/kg per minute for 6 minutes and 99MmTc-MIB1 was injected at 3 minute. We then compared the hemodynamic changes, side effects, scintigraphic and angiographic findings. Heart rate increased 90 ± 19 beats/minute in the group with ST depression compared with 80 ±16 beats/minute in the group without ST depression(p9mTc-MIBI images were abnormal in 23(96%) patients with ST segment depression and 66(69%) patients without ST segment depression(p99mTc-MIBI myocardial perfusion scintigraphy with intravenous adenosine is related to the severity of coronary artery disease.

  20. Planar scintimammography 99mTc-MIBI in breast cancer. Part 1. Review of literature and studies in healthy women

    International Nuclear Information System (INIS)

    After reviewing of the literature concerning 99mTc-MIBI and scintimammography (SCM) using this tracer, the results of planar technique obtained in 40 ''healthy'' women chosen from the candidates for myocardial perfusion study are presented. The aim of the study was to assess the normalcy rate, e.g. the percentage of normal images in women with minimal risk of breast cancer and the analysis the sources of potential overdiagnosis changes caused by this disease. Two observers an oncologists and a nuclear medicine physician) reviewed scintigrams of both sides of the chest (80 images) focusing on the regions where changes caused by breast cancer may happen to occur. Positive approval as normal met 95% images of the breast and subclavicular region, 82.5% of the axilla, 97% of the supraclavicular region and 96% of the parasternal region. In 2 women prominent ductal pattern was noted. In 7 cases focal uptake in the axilla was observed (on 4 in the same side as injection) and in further 7 patients increased accumulation in the infero-external part of the axilla was shown (the muscles?). In 3 patients foci of increased uptake were noted in the parasternal region. Two cases of symmetrically increased activity in suclavicular region and 1 in supraclavicular region were recognized as due to overexpression. Planar SCM is specific enough to be used in the breast cancer patients, although avoiding of overexpression of the images and caution in interpretation of the results (especially for the axilla) is recommended. Nonspecific accumulation of the tracer in various conditions may lead to false positive diagnosis, therefore an interview about trauma and possible inflammatory or vasculary changes is mandatory with the analysis of other additional results. (author)

  1. Assessment of cardiac outcome in pancreas kidney transplant recipients using 99mTc-MIBI myocardial perfusion scintigraphy

    International Nuclear Information System (INIS)

    Full text: Simultaneous pancreas-kidney transplantation (SPK) in diabetic patients (patients) 99mTc-MIBI myocardial perfusion scintigraphy (MPI) in predicting cardiac outcome post SPK. One hundred and forty patients underwent SPK at our institution over a 15-year period. Sixty-six subjects were excluded because of incomplete follow-up (n=54) or death from non-cardiac causes (n=12). Seventy-four patients (44 men, 30 women) with age 44.3+/-7.4 yrs (range: 31-62yrs) were followed for up to 14 yrs post SPK. The mean interval between SPK and MPI was 48.1+/-37.1 months (median: 37.5, range: 6-132 months). MPI was performed using a standard two-day protocol with Adenosine or Dipyridamole and a dual-head gamma camera using 900 configuration, attenuation and scatter correction and resolution recovery. Images were reported by at least one experienced observer using standard software. MPI were classified as normal (n=33), infarct (n=4), small (n=32) or large (n=2) areas of impaired coronary flow reserve (CFR) or a combination of infarct and impaired CFR (n=3). Cardiac events were considered if infarct, angina, death or coronary revascularisation occurred. Two patients underwent coronary artery bypass surgery five yrs post SPK (both two yrs post MPI) and one patient developed angina six yrs post SPK (three yrs post MPI). In these patients, MPI showed only small areas of impaired CFR. The negative and positive predictive values for MPI were 100 and 9%, respectively. In summary, MPI has excellent NPV post SPK. The PPV is poor, suggesting that myocardial perfusion defects correlate only weakly with overall atherosclerotic risk profile post SPK. Copyright (2002) The Australian and New Zealand Society of Nuclear Medicine Inc

  2. A comparison of radionuclide thyroid angiography, {sup 99m}Tc-MIBI scintigraphy and power Doppler ultrasonography in the differential diagnosis of solitary cold thyroid nodules

    Energy Technology Data Exchange (ETDEWEB)

    Demirel, Koray; Kapucu, Oezlem [Department of Nuclear Medicine, Gazi University School of Medicine, Ankara (Turkey); Yuecel, Cem; Oezdemir, Hakan [Department of Radiology, Gazi University School of Medicine, Ankara (Turkey); Ayvaz, Goeksun [Department of Endocrinology and Metabolic Diseases, Gazi University School of Medicine, Ankara (Turkey); Taneri, Ferit [Department of Surgery, Gazi University School of Medicine, Ankara (Turkey)

    2003-05-01

    We prospectively studied 43 patients with solitary cold thyroid nodules greater than 1.5 cm in diameter to determine the comparative diagnostic value of radionuclide thyroid angiography (RTA), technetium-99m methoxyisobutylisonitrile ({sup 99m}Tc-MIBI) scintigraphy and power Doppler ultrasonography (PDUS) in the differentiation of benign and malignant thyroid nodules. Perfusion of the nodules in RTA was compared with the perfusion in the surrounding normal thyroid tissue and classified as follows: 0, avascular; 1, hypovascular; 2, isovascular; 3, hypervascular. {sup 99m}Tc-MIBI uptake in the nodules compared with that in surrounding thyroid tissue was scored for both early and delayed images as follows: 0, cold; 1, decreased; 2, equal; 3, increased. PDUS patterns were classified as nodule vascularisation patterns. The malignancy criteria were set as follows: hypervascular nodule with rapid washout in RTA; complex ring sign with anarchic structure or delta sign in PDUS, and positive retention and increased uptake in the nodule in the early and delayed {sup 99m}Tc-MIBI images. These data were compared with the histopathological results. Histology revealed thyroid carcinoma in nine patients (five cases of papillary carcinoma, three of follicular carcinoma and one of medullary carcinoma) and benign conditions in 34 patients (30 cases of nodular goitre, three of lymphocytic thyroiditis and one of follicular adenoma). Sensitivity, specificity and accuracy were, respectively, 0.89, 1.00 and 0.97 for RTA, 1.00, 0.76 and 0.81 for PDUS, and 0.67, 0.91 and 0.86 for {sup 99m}Tc-MIBI scintigraphy (when nodules with increased uptake in both the early and the delayed images and a positive retention index were considered as malignant). RTA, {sup 99m}Tc-MIBI scintigraphy and PDUS could be helpful in the preoperative assessment of solitary cold thyroid nodules. In this study, RTA was found to be the most accurate and specific method for differentiation of malignant from benign

  3. Lack of correlation between Tc-99m-sestaMIBI uptake and cadherin expression in infiltrating ductal breast carcinoma as prognostic indicators

    International Nuclear Information System (INIS)

    Despite using various kinds of prognostic indicators, it is still not possible to predict the biological behavior of breast cancer in all patients. Tc-99m-sestaMIBI (MIBI) uptake determined by breast scintigraphy and cadherin expression of tumor tissue revealed by immunohistochemistry are suggested as potential agents for this purpose. We hypothesize that there can be a correlation between MIBI whose cellular mitochondrial content is claimed to play a significant role in its tumor uptake and cadherin whose downregulation causes an increase in mitochondrial activity in human mammary carcinoma cell lines. The aim of this study was to assess the relationship between the degree of MIBI tumor uptake and cadherin expression in infiltrating ductal breast carcinoma. Correlation with response to chemotherapy and some known prognostic factors of breast cancer such as tumor size, number of metastatic axillary lymph nodes and microscopic grading was also done. Fourteen patients who underwent scintimammography and subsequent surgical excisional biopsy that revealed infiltrating ductal carcinoma were enrolled in this study. Statistical analysis did not show any correlation between MIBI uptake and cadherin expression (p>0.05). Also, no statistically significant correlation was noted between MIBI uptake and tumor size, number of metastatic lymph nodes, microscopic grade, stage of the disease or response to chemotherapy. Similarly, there was no statistically significant correlation between cadherin expression and tumor size, number of metastatic lymph nodes, microscopic grade, stage of the disease or chemotherapy response. The results of this study imply that there is no correlation between MIBI tumor uptake and cadherin expression with neither of them good enough to be used as prognostic indicators for breast cancer. (author)

  4. 99mTc-MIBI Lung Scintigraphy in the Assessment of Pulmonary Involvement in Interstitial Lung Disease and Its Comparison With Pulmonary Function Tests and High-Resolution Computed Tomography: A Preliminary Study.

    Science.gov (United States)

    Bahtouee, Mehrzad; Saberifard, Jamshid; Javadi, Hamid; Nabipour, Iraj; Raeisi, Alireza; Assadi, Majid; Eftekhari, Mohammad

    2015-11-01

    The differentiation of active inflammatory processes from an inactive form of the disease is of great value in the management of interstitial lung disease (ILD). The aim of this investigation was to assess the efficacy of 99mTc-methoxy-isobutyl-isonitrile (99mTc-MIBI) scans in distinguishing the severity of the disease compared to radiological and clinical parameters.In total, 19 known cases of ILD were included in this study and were followed up for 1 year. Five patients without lung disease were considered as the control group. The patients underwent pulmonary function tests (PFTs) and high-resolution computed tomography scans, followed by 99mTc-MIBI scanning. The 99mTc-MIBI scans were analyzed either qualitatively (subjectively) or semiquantitatively.All 19 ILD patients demonstrated a strong increase in 99mTc-MIBI uptake in the lungs compared to the control group. The 99mTc-MIBI scan scores were higher in the patient group in both the early phase (0.24[0.19-0.31] vs 0.11[0.10-0.15], P 0.14). The 99mTc-MIBI scan scores were not significantly correlated with the PFT findings (P > 0.05). In total, 5 patients died and 14 patients were still alive over the 1-year follow-up period. There was also a significant difference between the uptake intensity of 99mTc-MIBI and the outcome in the early phase (dead: 0.32[0.29-0.43] vs alive: 0.21[0.18-0.24], P lung scans might distinguish the severity of pulmonary involvement in early views, which were well correlated with HRCT findings. These results also revealed that 99mTc-MIBI lung scans might be used as a complement to other diagnostic and clinical examinations in terms of functional information in ILD; however, further investigations are strongly required.

  5. Sympathetic reinnervation in cardiac transplants: 123I-MIBG and 201Tl/99mTc-MIBI scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Kim, J. H.; Oh, S. J.; Son, M. S.; Son, J. W.; Koh, K. K.; Choi, I. S.; Shin, E. K.; Park, K. Y. [Gachon Medical College, Gil Heart Center, Inchon (Korea, Republic of)

    1998-07-01

    Iodine-123 metaiodobenzylguanidine (123I-MIBG) is a norepinephrine (NE) analogue and taken up by myocardial sympathetic nerves. To determine whether cardiac sympathetic reinnervation occurs after orthotopic heart transplantation (TPL). We performed 24 serial or followup cardiac 123I-MIBG imaging and 201T1/99mTc-MIBI dipyridamole stress gated myocardial perfusion SPECT (g-MPS) in 15 pts(M : F =10 : 5; mean ages = 34.67{+-}12.92 yr; idiopathic: rheumatic=14:1) (10.80{+-}11.88 (1-48) mo) after TPL. 123I-MIBG imagins were performed in anterior position 15 minutes, 4 and 24 hours after i.v. injection of 148 MBq 123I MIBG. Image quantitation was based on the ratio of heart to mediastinal MIBG uptake (HMR). 12 subjects with < 13 (4.91{+-}3.67) months after TPL had no visible 123I-MIBG uptake on early 15 min imaging however, 12 subjects with 13 to 48(28.58{+-}12.77) months had visible cardiac 123I-MIBG uptake (HMR: 1.65 {+-}0.21 vs. 1.32{+-}0.26 p=0.002). Correlation was found between plasma NE concentration and HMR ( r=0.80: p<0.05). Compared to HMR on 15 min images (1.48{+-}0.28), neither four nor 24 hour delayed images (1.26{+-}0.23 vs. 1.06{+-}0.10 : p<0.05, respectively, ANOVA) showed definite delayed localization of MIBG. To dipyridamole stress, transplant hearts showed significant subnormal hemodynamic responses of HR, s-BP, d-BP, and rate pressure product (90.9{+-}14.9 to 102.2{+-}15.3, 136.5{+-}17.3 to 124.9{+-}13.3, 83.3{+-}12.5 to 74.7{+-}15.6, 123.2{+-}19.4 to 127.4{+-}21.8 p<0.05, respectively). One-year followup 123I-MIBG scintigraphy in nine pts showed increased HMR (1.50{+-}0,37 to 1.61{+-}0.15, p=ns) but couldnt reach the statistical significance. Out of nine followup patients, five showed increased HMR but four didnt. gMPS performed at post-TPL 48 months in one patient complaining vague chest pain whose HMR value 1.73 to 1.62 showed an apicoanterior wall reversible perfusion defect which confirmed as 90% distal left anterior descending artery stenosis by

  6. Sensitivity of parathyroid scintigraphy with Tc99m-MIBI (two phase) for localization of parathyroid adenoma

    International Nuclear Information System (INIS)

    Hyperparathyroidism is caused by increment of serum parathyroid Hormone and parathyroid adenoma is the underling pathology in 85 to 90% of cases. In 9 to 15% of cases etiology is parathyroid Hyperplasia. Treatment is surgical resection of abnormal gland. Nuclear medicine method is one of the best methods for localization of abnormal parathyroid gland. All patients who referred for parathyroid scan to nuclear medicine department were studied. After complete history and thyroid examination, the patients were injected with 20 mCi-Tc-99m-MIBI intravenously and imaging were performed in 2 seconds intervals for 2 minutes than one minute images for 10 minutes and another 10 minutes image was recorded. Also delayed image was down at 2 hours. Forty eight patients were studied. Parathyroid scan was negative in 26 patients and positive in 22 patients. Chief complaint was general malaise in 37.8% of patients. From 14 patients who have adenoma in pathology, in 11 patients adenoma were accurately localized and 3 patients had negative scan. From 4 cases with parathyroid hyperplasia only one case had positive scan and 3 foci was detected in scintigraphy. Sensitivity of scan for diagnosis and localization of parathyroid adenoma was 78.5% and sensitivity for diagnosis of hyperplasia was only 33.3%. Serum Ca level was significantly higher inpatients with parathyroid adenoma (10.4 mg/d L) in scintigraphy compared with scan negative patients (9.5 mg/d L). (P=0.02) Respectively serum phosphorus level was 2.5 mg/d L in patients with adenoma and 3.4 mg/dl in other patients. (P=0.003). Serum T H level was 316.2 I U in patients with adenoma and 191.4 I U in other patients which was statistically significant (P=0.03). Conclusion: Parathyroid scan is recommended for all patients undergoing parathyroid surgery with respect high sensitivity for localization of parathyroid adenoma and fairly low cost. The mean serum Ca in patients with parathyroid adenoma was 10.4 mg/d L which is far less than

  7. Assessing Response Using Tc99m-MIBI Early after Interstitial Chemotherapy with Carmustine-Loaded Polymers in Glioblastoma Multiforme: Preliminary Results

    Directory of Open Access Journals (Sweden)

    D. Cecchin

    2014-01-01

    Full Text Available Introduction. Early signs of response after applying wafers of carmustine-loaded polymers (gliadel are difficult to assess with imaging because of time-related imaging changes. Tc99m-sestamibi (MIBI brain single-photon emission tomography (SPET has reportedly been used to reveal areas of cellularity distinguishing recurrent neoplasm from radionecrosis. Our aim was to explore the role of MIBI SPET in assessing response soon after gliadel application in glioblastoma multiforme (GBM. Methods. We retrospectively reviewed the charts on 28 consecutive patients with a radiological diagnosis of GBM who underwent MIBI SPET/CT before surgery (with intracavitary gliadel placement in 17 patients, soon after surgery, and at 4 months. The area of uptake was selected using a volume of interest that was then mirrored contralaterally to obtain a semiquantitative ratio. Results. After adjusting for ratio at the baseline, the effect of treatment (gliadel versus non-gliadel was not statistically significant. Soon after surgery, however, 100% of patients treated with gliadel had a decreased ratio, as opposed to 62.5% of patients in the non-gliadel group P=0.0316. The difference between ratios of patients with radical versus partial resection reached statistical significance by a small margin P=0.0528. Conclusions. These data seem to suggest that the MIBI ratio could be a valuable tool for monitoring the effect of gliadel early after surgery.

  8. Reversal of multidrug resistance with KR-30035: evaluated with biodistribution of Tc-99m MIBI in nude mice bearing human tumor xenografts

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jung Kyun; Lee, Jae Tae; Lee, Byung Ho [Kyungpook National Univ. Hospital, Taegu (Korea, Republic of)] [and others

    2001-06-01

    KR-30035 (KR), a new MDR reversing agent, has been found to produce a similar degree of increased Tc-99m MIBI uptake in cultured tumor cells over-expressing mdr1 mRNA compared to verapamil (VP), with less cardiovascular effects. We assessed the MDR-reversing ability of KR in vivo, and effects of various doses of KR on MIBI uptake in nude mice bearing P-glycoprotein (P-gp) positive (+) and P-gp negative (-) human tumor xenografts. P-gp (+) HCT15/CLO2 colorectal and P-gp (-) A549 non-small cell cancer cells were inoculated in each flank of 120 nude mice (20 mice x 6 groups). Group 1 (Gr1) mice received 10mg/kg Kr i.p. 3 times (x3); Gr2, 10mg/kg VP i.p. x3; Gr3, 10mg/kg KR i.p. x2 + 25mg/kg KR i.p. x1; Gr4, 10mg/kg KR i.p. x 2 + 50mg/kg i.p. x1; Gr5, 10mg/kg Kr i.p. x2 + 25mg/kg KR i.v. x1, GrC, controls. The mice were then injected with Tc-99m MIBI and sacrificed after 10 min, 30 min, 90 min and 240 min. Tumor uptake of MIBI (TU) in each group was compared. Tu in P-gp (+) and (-)tumors were both higher in Gr1 than Gr2. Washout rate between the 10 min and 4 hours was lower in Gr5 of P-gp (+) cell (0.93) than the control. Percentage increases in Tu were higher in P-gp (+) than P-gp (-) tumors with all KR doses. Pgp (+) TU were highest at 10 min (173% of GrC) and persisted up to 240 min (144%) in Gr3. Larger doses of KR resulted in a lesser degree of increase in P-gp (+) TU at 10 min (130% in Gr4 and 117% in Gr5) and 30 min (178%, 129%), but TU increased by time up to 240 min (177%, 196%). Heart and lung uptakes were markedly increased in Gr4 and Gr5 at 10 and 3C min, likely due to cardiovascular effects. No mice died. These data further suggest that KR that has significantly lower cardiovascular toxicity than verapamil can be used as an active inhibitor of MDR. Even a relatively low dose of KR significantly increased Tc-99m MIBI uptake in P-gp (+) tumors in vivo.

  9. Early detection of radiation-induced heart disease using 99mTc-MIBI SPECT gated myocardial perfusion imaging in patients with oesophageal cancer during radiotherapy

    International Nuclear Information System (INIS)

    Background and purpose: The primary aim of this prospective study was to investigate the value of 99mTc-methoxyisobutylisonitrile (MIBI) single photon emission computed tomography (SPECT) gated myocardial perfusion imaging (GMPI) in the detection of radiation-induced heart disease (RIHD) as early as during radiotherapy (RT) for oesophageal cancer (EC). The second aim was to analyse the correlation between cardiac toxicity and the dose–volume factors. Materials and methods: The 99mTc-MIBI SPECT GMPI was performed both pre-RT and during RT (40 Gray). The results of the SPECT were quantitatively analysed with QGS/QPS software and read by two experienced nuclear medicine physicians. The correlation between the changes in the SPECT parameters and the RT dosimetric data was analysed. Results: Eighteen patients with locally advanced EC were enrolled in the study. Compared with the baseline, the imaging during RT showed not only significant decreases in the wall motion (WM) (1/20 segments), wall thickening (WT) (2/20 segments), end-diastolic perfusion (EDP) (5/20 segments) and end-systolic perfusion (ESP) (8/20 segments) (p < 0.05) but also a significant increase in the heart rate (74.63 ± 7.79 vs 81.49 ± 9.90, p = 0.036). New myocardial perfusion defects were observed in 8 of the 18 patients. The V37–V40 was significantly higher (p < 0.05) in the patients with the new perfusion defects during RT than in the patients who did not exhibit these defects. Conclusions: Radiotherapy for EC induces cardiac damage from an early stage. 99mTc-MIBI SPECT GMPI can detect the occurrence of cardiac impairment during RT. The WM, WT, EDP and ESP may be valuable as early indicators of RIHD. The percentage of the heart volume that receives a high dose is an important factor that is correlated with RIHD

  10. Is technetium-99m-MIBI taken up by the normal pituitary gland? A comparison of normal pituitary glands and pituitary adenomas

    International Nuclear Information System (INIS)

    The aim of this study was to compare the behavioral uptake of a normal gland and a pituitary adenoma and to assess the ability to diagnose pituitary adenoma by means of technetium-99m-hexakis-2-methoxy-isobutyl-isonitrile (MIBI) single photon emission computed tomography (SPECT). The study included 15 patients with pituitary adenomas (mean age=44.0 years, range 19-63) and 15 control subjects (mean age=50.7 years, range 20-67). SPECT was performed 15 minutes after an intravenous injection of MIBI 600 MBq. The shape and location of MIBI uptake were evaluated on a magnetic resonance (MR) imaging/SPECT registration image. The shape patterns and location were classified as follows: Shape C (circular); LO (longitudinal oval); T/R (triangular or rectangular) and location P (pituitary gland or adenoma); D/C (dorsum sellae and/or clivus). Analysis of the uptake showed that 10 (67%) adenomas were C, and 5 (33%) were LO. Of the controls, 5 (33%) were C, and 10 (69%) were T/R. With regard to location, all patients with pituitary adenomas were classified as P, and all control subjects (93%) but one showed uptake in the dorsum sellae and clivus (D/C). MIBI was taken up in the dorsum sellae or clivus but not the normal pituitary gland and had a strong affinity for the pituitary adenoma. This result implies that MIBI SPECT may be a useful new auxiliary examination technique for the location diagnosis of pituitary adenoma. (author)

  11. Evaluation of the influence of percent labeling of 99mTc-MIBI on nuclear medicine procedures in Recife, Pernambuco State, Brazil

    International Nuclear Information System (INIS)

    The aim of this work was to evaluate the influence of percent labeling of the radiopharmaceutical 99mTc-MIBI on the procedures adopted by the nuclear medicine clinics in Recife, Pernambuco State, Brazil, and their possible interference with image quality in myocardial perfusion examinations. This work was performed at two nuclear medicine services in Recife that use pharmaceuticals provided by two different manufacturers: Cardiolite, provided by Dupont and Cardiosyd, provided by SYDMA. The pH, percent labeling and stability of samples of 99mTc-MIBI and the percent uptake in heart and liver of a number of patients were evaluated. The results showed that the pH values measured in all samples of both radiopharmaceuticals were within the limits recommended. In 48% of the Cardiolite samples, the percent labeling was less than 90%, which is the minimum recommended limit. On the other hand, 87,5% of the Cardiosyd samples measured values above 98%. Both radiopharmaceuticals had good labeling stability, even in samples with low percent labeling efficiencies. In spite of the higher percent labeling of Cardiosyd its heart uptake is similar to that observed with the Cardiolite, even when the percent labeling is lower than 90%. On the other hand, the image quality, according to physicians, evaluation was poorer for Cardiosyd images, due to high noise and poor sharpness. (author)

  12. Long-term prognostic value of exercise {sup 99m}Tc-MIBI SPET myocardial perfusion imaging in patients after percutaneous coronary intervention

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Xiaoli; Liu, Xiujie; He, Zuo-Xiang; Shi, Rongfang; Yang, Minfu; Gao, Runlin; Chen, Jilin; Yang, Yuejin; Fang, Wei [Cardiovascular Institute and Fu Wai Hospital, PUMC and CAMS, A 167 Beilishilu, 100037, Beijing (China)

    2004-05-01

    The purpose of this study was to evaluate the long-term prognostic value of exercise technetium-99m methoxyisobutylisonitrile ({sup 99m}Tc-MIBI) single-photon emission tomography (SPET) imaging in patients after percutaneous coronary intervention (PCI). Three hundred and eighteen consecutive post-PCI patients who underwent exercise and rest {sup 99m}Tc-MIBI SPET myocardial perfusion imaging (MPI) were followed up for 38{+-}27 months. Patients with early revascularisation (<3 months after MPI) were excluded. A semiquantitative visual analysis employing a 20-segment and four-point scoring system was used to define the summed stress score (SSS), summed rest score (SRS) and summed difference score (SDS). Death and non-fatal myocardial infarction (MI) were considered as hard events, and late revascularisation procedures ({>=}3 months after MPI) as soft events. Fifty-one patients (16.0%) suffered from cardiac events during follow-up, including 1 (0.3%) death, 13 (4.1%) non-fatal MIs, 9 (2.8%) coronary artery bypass grafting procedures and 28 (8.8%) PCIs. According to the SPET results, patients were classified into three groups: patients with normal MPI (SSS=0, n=153), patients with irreversible defects (SDS<3 and SRS>1, n=100) and patients with reversible defects (SDS{>=}3, n=65). The annual hard cardiac event rate in patients with reversible defects was 3.9%, which was significantly higher than that in patients with normal MPI (0.2%, {chi}{sup 2}=7.71; P<0.01). The annual soft cardiac event rate in patients with reversible defects was 10.7%, which was significantly higher than that in patients with irreversible defects (2.5% {chi}{sup 2}=17.69; P<0.001), and also significantly higher than that in patients with normal MPI (1.5%, {chi}{sup 2}=33.89; P<0.001). In patients with normal and reversible defects, there was no significant difference in soft and hard cardiac event rates according to whether patients were symptomatic or asymptomatic (P>0.05). However, the annual

  13. The role of 99mTc-SestaMIBI in predicting post-revascularization recovery in patients with chronic coronary artery disease and LV dysfunction: a meta-analysis

    International Nuclear Information System (INIS)

    The utility of 99mTc-Sesta (MIBI) as a marker of myocardial viability is still a controversial topic. A perusal of the different published studies was therefore done to address this issue by looking at the ability of this tracer to predict recovery or non-recovery of a dysfunctional myocardium after revascularization based on MIBI uptake. Literature search was done using the Index Medicus, MEDLINE and cross-reference on the use of 99mTc-SestaMIBI in thrombolytic therapy, revascularization and viability assessment. Only studies in human were included. A qualitative and quantitative (visual) uptake was studied. These segments are supposedly expected to have no recovery even after revascularization. Eventual revascularization was done however on their corresponding coronary vessels. 57% (78/136) showed recovery in function while only 43% (58/136) were identified correctly (p>0.05=ns). Quantitative analysis of uptake were done on these segments with poor visual uptake. One study (Marzullo et.al.) divided these segments into those with MIBI uptake above and below 55%. His findings revealed that 35/45 segments with uptake >55% had improved function after revascularization (PPV=78%) while 14/21 segments with uptake 50% showed functional improvement while 61% (NPV) of segments with uptake 99mTc-SestaMIBI is primarily a perfusion agent. Attempts therefore to use this tracer to asses myocardial viability should be made with caution and if ever, must be done quantitatively. (author)

  14. Evaluation of the quality of the radiopharmaceutical 99mTc-MIBI and its influence on image quality in myocardial perfusion scintigraphy

    International Nuclear Information System (INIS)

    This study evaluated the quality of the 99mTc-MIBI radiopharmaceutical from different manufacturers, used in three nuclear medicine services (NMS) in Recife-PE, through labeling procedure of each service. It was observed their biodistribution by quantifying the activity present in the organs of interest (heart / liver), the influence and interference in image quality and in myocardial scintigraphy diagnosis exam. In these NMS (A, B and C) were done quality controls in the eluates of 99Mo/99mTc generators (radionuclidic, chemical and radiochemical purity and pH) and of the 99mTc-MIBI radiopharmaceutical (radiochemical purity and pH) used in myocardial scintigraphy exam. In the case of radiochemical purity (RCP), was used the thin layer chromatography technique; after the chromatographic ran on, the plates were analyzed both in the dose calibrator, and in scintillation camera of each NMS. The radiopharmaceutical biodistribution was evaluated through the activities present in the heart and liver images in 60 patients, using the technique of combined images counting. Five nuclear physicians analyzed 24 images through myocardial perfusion visual interpretation during stress, it was verified the agreement degree among them. The results of the quality control showed that all eluate samples were in agreement with the manufacturers in relation to radionuclidic purity and pH. In relation to chemical purity, 10% of the services samples B and C showed Al+3 values above 10 ppm. In the RCP, it was observed that using the scintillation camera, only 22% of the samples would be discarded, while with dose calibrator would be 78%, indicating that the scintillation camera is more sensitive in chromatographic pale analysis. For the labeled radiopharmaceutical, the services B and C presented respectively one and three samples with RCP percentage below 90%. However, C service presented the lowest medium to liver/heart proportions, showing that this factor does not depends on the labeling

  15. Comparison of the kinetics of active efflux of Tc-99m-MIBI in cells with P-glycoprotein-mediated and multidrug-resistance protein-associated multidrug-resistance phenotypes

    NARCIS (Netherlands)

    Vergote, J; Moretti, JL; De Vries, EGE; Garnier-Suillerot, A

    1998-01-01

    The overexpression of two membrane glycoproteins, P-glycoprotein and multidrug-resistance protein (MRP1) is a major cause of resistance to chemotherapeutic agents in the treatment of human cancers. Both proteins confer a similar multidrug-resistant (MDR) phenotype. Tc-99m-MIBI, a myocardial imaging

  16. Evaluation of the influence of percent labeling of {sup 99m}Tc-MIBI on nuclear medicine procedures in Recife, Pernambuco State, Brazil; Avaliacao da influencia do percentual de marcacao do {sup 99m}Tc-MIBI em procedimentos de medicina nuclear em Recife, PE

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Jucilene Maria

    2003-08-15

    The aim of this work was to evaluate the influence of percent labeling of the radiopharmaceutical {sup 99m}Tc-MIBI on the procedures adopted by the nuclear medicine clinics in Recife, Pernambuco State, Brazil, and their possible interference with image quality in myocardial perfusion examinations. This work was performed at two nuclear medicine services in Recife that use pharmaceuticals provided by two different manufacturers: Cardiolite, provided by Dupont and Cardiosyd, provided by SYDMA. The pH, percent labeling and stability of samples of {sup 99m}Tc-MIBI and the percent uptake in heart and liver of a number of patients were evaluated. The results showed that the pH values measured in all samples of both radiopharmaceuticals were within the limits recommended. In 48% of the Cardiolite samples, the percent labeling was less than 90%, which is the minimum recommended limit. On the other hand, 87,5% of the Cardiosyd samples measured values above 98%. Both radiopharmaceuticals had good labeling stability, even in samples with low percent labeling efficiencies. In spite of the higher percent labeling of Cardiosyd its heart uptake is similar to that observed with the Cardiolite, even when the percent labeling is lower than 90%. On the other hand, the image quality, according to physicians, evaluation was poorer for Cardiosyd images, due to high noise and poor sharpness. (author)

  17. Comparison of 18F-FDG PET/CT, 99mTc-methy-isobutyl-isonitrile(MIBI) scan and radioiodine diagnostic whole body scan with I-131 post-therapeutic whole body scan in papillary thyroid carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Chung Ho; Park, Young Ha; Yoo, Ie Ryung; Kim, Sung Hoon; Chung, Soo Kyo [The Catholic University of Korea, Seoul (Korea, Republic of)

    2007-07-01

    To evaluate the usefulness of 18F-FDG PET/CT and 99mTc-MIBI scan in the detection of remnant thyroid tissue, recurrence or metastasis before high dose I-131 ablation therapy. Twenty-four patients (20 female, 4 male, age=52.913.2), who received 99mTc-MIBI scan, radioiodine diagnostic whole body scan (WBS) and 18F-FDG PET/CT within a week before high dose I-131 ablation therapy from May 2004 to September 2007 were retrospectively reviewed. All patients thyroid stimulating hormone, thyroglobulin (Tg) and anti-thyroglobulin level were checked before administering I-123 or I-131 for diagnostic WBS. TSH level were higher than 30 mIU/L in all patients. The radioiodine diagnostic WBS, 99mTc-MIBI scan and 18F-FDG PET/CT were compared with the post-therapeutic WBS obtain 7 days after administration of I-131 (=150 mCi). Post-therapeutic WBS of 4 patients showed distant metastases, commonly in lung, all 4 were positive on 18F-FDG PET/CT, 1 on 99mTc-MIBI scan, but none of them were detectable on radioiodine diagnostic WBS. 7 patients with cervical or supraclavicular lymph node metastases on post-therapeutic WBS, 5 were positive on 18F-FDG PET/CT, 4 on radioiodine diagnostic WBS, and 3 on 99mTc-MIBI scan. 9 patients with remnant thyroid tissue only in thyroid bed, 7 were positive on radioiodine diagnostic WBS, 5 on 18F-FDG PET/CT and 3 on 99mTc-MIBI scan. One case with high Tg level (>10ng/mI) was positive only on post-therapeutic WBS scan. 3 patients with high Tg level, all of diagnostic scans and post-therapeutic scan were negative. Compared to the post-therapeutic WBS with radioiodine diagnostic WBS, 99mTc-MIBI scan, 18F-FDG PET/CT, none of the studies were perfectly matched with post-therapeutic WBS, but can compensate each other. 18F-FDG PET/CT might be helpful, when lymph node or lung metastasis is suspected. Further evaluation with larger population is necessary.

  18. Parathyroid ectopia evidenced by {sup 99m}Tc-sestamibi scintigraphy: Case report; Ectopie parathyroidienne objectivee par la scintigraphie au {sup 99m}Tc-MIBI: a propos d'un cas

    Energy Technology Data Exchange (ETDEWEB)

    Ghfir, I.; Ben Rais, N. [Hopital Ibn-Sina, Service de Medecine Nucleaire (Morocco)

    2007-08-15

    Introduction The presence of an ectopic parathyroid gland is the most frequent cause of persistent hyperparathyroidism. Its detection constitutes a source of topographic diagnostic difficulties particularly for purely morphological explorations. The goal of this work is to elucidate the interest of scintigraphy using {sup 99m}Tc-sestamibi in the diagnosis and the therapeutic orientation of ectopic parathyroid. Case report A 50-year-old patient was followed for persistent hyperparathyroidism after subtotal para thyroidectomy. The clinical examination was without particularity contrasting with an always high parathormone rate. Echography, computed tomography and magnetic resonance imaging were not conclusive. Scintigraphy carried out after intravenous injection of 740 MBq of sestamibi-Tc{sup 99m} with acquisition of static images at 20 min and 2 h showed an area of high uptake in projection of the right upper mediastinum. The diagnosis of an ectopic parathyroid localization was retained. Resection and pathologic examination of the surgical specimen revealed parathyroid adenoma tissue. The evolution was marked by a normalisation of the rate of parathormone. Discussion {sup 99m}Tc-sestamibi scintigraphy is a functional exploration with a higher sensitivity than the other morphological imaging techniques for preoperative topographic determination of ectopic parathyroid. It significantly contributes to an elective and more rapid surgical resection of these lesions. (authors)

  19. Synthesis of the compound tetrakis (2- methoxy isobutyl isonitrile) copper (I) tetrafluoroborate -(Cu[MIBI]4BF4): substance used in the production of the radiopharmaceutical sestamibi 99m Tc

    International Nuclear Information System (INIS)

    The radiopharmaceutical SESTAMIBI-99mTc presents interest and application in the clinical routine in Nuclear Medicine, especially for evaluation of the myocardium function and in the diagnosis of some tumors types, and the Center of Radiopharmacy of the IPEN has interest in the development of this radiopharmaceutical for attendance of the nuclear medicine class necessities. In this work it was demonstrated the synthesis and characterization of the active compound tetrakis (2- methoxy isobutyl isonitrile) copper (I) tetrafluoroborate - Cu[MIBI]4BF4- to be used in the preparation of lyophilized reagents for labeling with technetium-99m as well as labeling studies and biological distribution. The synthesis of the reagent Cu[MIBI]4BF4 was carried out in accordance with previously described procedures, in 5 steps and the compounds were characterized for infra-red, magnetic nuclear resonance, melting point and thin layer chromatography. The characterization of the chelate of MIBI -Cu[MIBI]4BF4 - was carried out using high performance liquid chromatography, compared with a standard sample of the product and presented similar results, as described in literature. The lyophilized reagent was prepared and labeled with technetium-99m, showing high radiochemical purity, higher than 97%, with high stability. Biological distribution in different animals showed good uptake in the heart, in time compatible with the acquisition of scintigraphy images, demonstrating the applicability of the reagent synthesized in the attainment of cardiac scintigraphy images. (author)

  20. Noninvasive stress testing of myocardial ischemia: comparison of GRE-MRI perfusion and wall motion analysis to 99mTc-MIBI-SPECT, relation to coronary angiography

    International Nuclear Information System (INIS)

    In the evaluation of ischemic heart disease only MR imaging seems to have the potential to assess myocardial perfusion, function, and coronary morphology on a single instrument. The aim of this study was to assess the feasibility of a stress test with dipyridamole (0.56 mg/kg) to analyze myocardial perfusion by Gd first-pass enhancement in ultrafast gradient-recalled-echo MRI (perf-MRI), and wall motion by cine gradient-recalled-echo MRI (Cine-MRI) in one imaging session. Twelve patients underwent complete rest and stress studies; satisfactory MR images were acquired in 10 patients. By 99mTc-MIBI-SPECT sensitivities to detect ischemic segments were 66.7 % with Perf-MRI, 80.0 % with WM-MRI and 86.7 % for Perf-WM-MRI (Perf-MRI vs Perf-WM-MRI; p = 0.03). Scar was equally detected with a sensitivity of 91.6 % with either MRI technique. Thus, Perf-Cine-MRI provides complementary information for the management of ischemic heart disease and has a higher sensitivity than Perf-MRI alone. (orig.). With 3 figs., 2 tabs

  1. Comparison of whole-body {sup 18}F-FDG PET, {sup 99m}Tc-MIBI SPET, and post-therapeutic {sup 131}I-Na scintigraphy in the detection of metastatic thyroid cancer

    Energy Technology Data Exchange (ETDEWEB)

    Iwata, Masahiro [Department of Radiology, Hikone Municipal Hospital, 1882 Hassakacho, 522-8539, Hikone, Shiga (Japan); Kasagi, Kanji [Department of Nuclear Medicine, Takamatsu Red Cross Hospital, Takamatsu (Japan); Misaki, Takashi [Radioisotope Center, Tenri Hospital, Tenri (Japan); Matsumoto, Keiichi; Nakamoto, Yuji [Institute of Biomedical Research and Innovation, Kobe (Japan); Iida, Yasuhiro; Ishimori, Takayoshi; Higashi, Tatsuya; Saga, Tsuneo; Konishi, Junji [Department of Nuclear Medicine and Diagnostic Imaging, Kyoto Graduate School of Medicine, Kyoto (Japan)

    2004-04-01

    The usefulness of fluorine-18 fluorodeoxyglucose (FDG) positron emission tomography (PET) in differentiated thyroid cancer (DTC) has been demonstrated by many investigators, but in only a small number of studies have FDG-PET images been compared with those obtained using other non-iodine tumour-seeking radiopharmaceuticals. In most of the studies, planar imaging was performed for comparison using thallium-201 chloride or technetium-99m 2-methoxyisobutylisonitrile ({sup 99m}Tc-MIBI). Furthermore, FDG-PET studies were not always performed in the hypothyroid state with increased levels of thyroid stimulating hormone (TSH), which are known to increase FDG uptake by DTC. The aim of this study was to compare the ability of FDG-PET to detect metastatic DTC with that of {sup 99m}Tc-MIBI whole-body single-photon emission tomography (SPET) and post-therapeutic iodine-131 scintigraphy, evaluated under TSH stimulation. Nineteen patients (8 men, 11 women; age range, 38-72 years, mean 60 years; 17 thyroidectomised and 2 inoperable patients following {sup 131}I ablation of the remaining thyroid tissue; 16 papillary and 3 follicular carcinomas) with metastatic DTC underwent FDG-PET whole-body scan (WBS) and {sup 99m}Tc-MIBI SPET WBS at an interval of less than 1 week, followed by {sup 131}I therapy. The SPET images were reconstructed using the maximum likelihood expectation maximisation (ML-EM) method. All patients were hypothyroid at the time of each scan. {sup 131}I WBS was performed 3-5 days after oral administration of the therapeutic dose. A total of 32 lesions [10 lymph node (LN), 15 lung, 6 bone, 1 muscle] were diagnosed as metastases, as confirmed by histopathology and/or other imaging modalities (X-ray, US, CT, MRI, bone, {sup 201}Tl and {sup 131}I scans). FDG-PET, {sup 99m}Tc-MIBI SPET and post-therapeutic {sup 131}I scintigraphy respectively revealed a total of 26 (81.3%), 20 (62.5%) and 22 (68.8%) lesions. These techniques respectively demonstrated nine (90.0%), eight

  2. Evaluation of the quality of the radiopharmaceutical 99mTc-MIBI and its influence on image quality in myocardial perfusion scintigraphy; Avaliacao da qualidade do radiofarmaco {sup 99m}Tc-MIBI e sua influencia na qualidade da imagem em cintilografia de perfusao do miocardio

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Poliane Angelo de Lucena

    2013-07-01

    This study evaluated the quality of the {sup 99m}Tc-MIBI radiopharmaceutical from different manufacturers, used in three nuclear medicine services (NMS) in Recife-PE, through labeling procedure of each service. It was observed their biodistribution by quantifying the activity present in the organs of interest (heart / liver), the influence and interference in image quality and in myocardial scintigraphy diagnosis exam. In these NMS (A, B and C) were done quality controls in the eluates of {sup 99}Mo/{sup 99m}Tc generators (radionuclidic, chemical and radiochemical purity and pH) and of the {sup 99m}Tc-MIBI radiopharmaceutical (radiochemical purity and pH) used in myocardial scintigraphy exam. In the case of radiochemical purity (RCP), was used the thin layer chromatography technique; after the chromatographic ran on, the plates were analyzed both in the dose calibrator, and in scintillation camera of each NMS. The radiopharmaceutical biodistribution was evaluated through the activities present in the heart and liver images in 60 patients, using the technique of combined images counting. Five nuclear physicians analyzed 24 images through myocardial perfusion visual interpretation during stress, it was verified the agreement degree among them. The results of the quality control showed that all eluate samples were in agreement with the manufacturers in relation to radionuclidic purity and pH. In relation to chemical purity, 10% of the services samples B and C showed Al{sup +3} values above 10 ppm. In the RCP, it was observed that using the scintillation camera, only 22% of the samples would be discarded, while with dose calibrator would be 78%, indicating that the scintillation camera is more sensitive in chromatographic pale analysis. For the labeled radiopharmaceutical, the services B and C presented respectively one and three samples with RCP percentage below 90%. However, C service presented the lowest medium to liver/heart proportions, showing that this factor

  3. Sympathetic reinnervation in cardiac transplants: {sup 123}I-MIBG and {sup 201}Tl/{sup 99m}Tc-MIBI scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Kim, J. H.; Oh, S. J.; Son, M. S.; Son, J. W.; Choi, I. S.; Shin, E. K.; Park, C. H. [Gachon Medical School, Gil Heart Cener, Inchon (Korea, Republic of)

    2000-07-01

    The purpose was to evaluate cardiac sympathetic reinnervation and hemodynamic changes after orthotopic heart transplantation (TPL). We performed 24 serial or followup cardiac 123I-MIBG imaging and rest 201Tl/99mTc-MIBI dipyridamole stress gated myocardial perfusion SPECT (g-MPS) in 15 patients (M:F=10:5;mean ages=34.5{+-}13.0 yr; idiopathic:rheumatic=14:1; one heart lung TPL)(10.80 {+-}11.88 (1-48) mo) after TPL 123I-MIBG imagings were performed in anterior position 15 minutes, 4 and 24 hours after i.v. injection of 148 MBq 123I MIBG. Image quantitation was based on the ratio of heart to mediastinal MIBG uptake (HMR) Compared to HMR on 15 min images (1.48 {+-} 0.28), neither four nor 24 hour delayed images (1.26 {+-} 0.23 vs. 1.06 {+-} 0.26: p<0.05, respectively, ANOVA) showed definite delayed localization of MIBG. 12 subjects with <13 (4.9 {+-}3.7) months after TPL had no visible 123I-MIBG uptake on early 15 min imaging however, 12 subjects with 13 to 48(28.6{+-}12.8) months had visible cardiac 123I-MIBG uptake (HMR: 1.65{+-}0.21 vs. 1.32{+-}0.26; p=0.002). One-year followup 123I-MIBG scintigraphy in nine pts showed significantly increased HMR(1.40{+-}0.31 to 1.61{+-}0.16, p<0.05) but a plateau was reached at HMR value of 2.0, which was still lower than 3.0 in normal controls. Plasma NE was increased according to I-123 MIBG myocardial uptake. Annual G-MPS detected an allograft atherosclerosis in one pt and showed progressive normalization of tachycardia and significant deterioration of LVEF and cardiac indices according to severity of rejection. To dipyridamole stress, transplant heats showed significant subnormal hemodynamic responses. Partial sympathetic late reinnervation can occur <1 year after TPL, and reached a plateau of two-third of normal value. G-MPS seems to be a useful screening test for the detection of allograft atherosclerosis and rejection.

  4. Sympathetic reinnervation in cardiac transplants : preliminary results {sup 123}I-MIBG and {sup 201}Tl/{sup 99m}Tc-MIBI scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Joug Ho; Oh, Se Jin; Son, Min Soo; Son, Ji Won; Choi, In Seok; Shin, Euk Kyun; Park, Kuk Yang; Kim, Ju E. [International Medicine and Thoraic Surgery, Inchon (Korea, Republic of)

    1997-07-01

    Iodine-123 metaiodobenzylguanidine ({sup 123}I-MIBG) is a norepinephrine (NE) analogue. To determine whether cardiac sympathetic reinnervation occurs after orthotopic heart transplantation (TPL). Nine patients (M : F=7 :2; mean ages=34{+-}24.1 yr; idiopathic:rheumatic = 8: 1) within 197.{+-}14.3 (4-36) months after TPL performed both {sup 123}I-MIBG scintigraphy and {sup 201}Tl/{sup 99m}Tc-MIBI dipyridamole stress gated myocardial perfusion SPECT (g-MPS). {sup 23}I-MIBG imagings were performed in anterior position 15 minutes, 4 and 24 hours after i.v. injection of 148 MBq {sup 123}I MIBG. Image quantitation was based on the ratio of hear to mediastinal MIBG uptake (HMR). Six subjects with <14 (4.3{+-}1.4) months after TPL had no visible {sup 123}I-MIBG uptake on early 15. min imaging however, three subjects with 26 to 36(32.0{+-}5.3) months had visible cardiac {sup 123}I-MIBG uptake (HMR:1.24{+-}0.09 vs. 1.8{+-}0.2). Correlation was found between plasma NE concentration and HMR(r=0.80: p<0.05). Compared to HMR on 15 min images (1.5{+-}0.3), neither four nor 24 hour delayed images (1.3{+-}0.3 vs. 1.1{+-}0.1 : p<0.05, respectively, ANOVA) showed definite delayed localization of MIBG. The uptakes in the liver, lung, salivary glands and spleen were present. To dipyridamole stress, transplant hearts showed significant subnormal hemodynamic responses of HR, s-BP, d-BP, and rate pressure product (95.4{+-}13.8 to 107.4{+-}14.6, 131.0{+-}16.7 to 123.6{+-}13.4, 79.1{+-}12.7 to 72.2{+-}12.7, 124.5{+-}19.6 to 133.0{+-}23.6 p<0.05, respectively). G-MPS of one patient shod an apicoanterior wall reversible perfusion defect which was confirmed as 90% distal left anterior descending artery stenosis by coronary angiography. MIBG uptake seems to involve mainly the specific sodium and energy dependent uptake-1 pathway, and the non-neuronal uptake-2 involving simple diffusion is not significant. Conclusively, partial sympathetic late reinnervation of the transplant human hearts can

  5. Labelling, biodistribution and compartmental analysis of N-acetylcysteine labelled with Tc-99m. Comparative investigation with with sup 9 sup 9 sup m Tc-MIBI in an in vivo tumoral model

    CERN Document Server

    Faintuch, B L

    1997-01-01

    Labelling and biodistribution studies were done with two different ligands, respectively Methoxy isobutyl isonitrile (MIBI) and N-acetylcysteine (NAC), employing Tc-99m as a tracer. The main objective was to assess the pharmacokinetic properties of the second substance, aiming at its possible application in cancer diagnosis. To this purpose an in vivo investigation was done using healthy and tumor-bearing rats with experimental cancer. Images of tumor-bearing rats registered in a scintillation camera indicated that with sup 9 sup 9 sup m Tc-MIBI none of the two selected times was adequate for visualization of the cancer mass. In contrast, sup 9 sup 9 sup m Tc-NAC permitted clear identification of the humor, four hours after injection. The results have demonstrated that sup 9 sup 9 sup m Tc-NAC is a radiopharmaceutical with affinity for cancer tissue and promising for further investigation concerning imaging diagnosis of tumors.

  6. Initial and delayed stress phase imaging in a single-injection double-acquisition SPECT. The potential value of early {sup 99m}Tc-MIBI redistribution in assessment of myocardial perfusion reversibility in patients with coronary artery disease

    Energy Technology Data Exchange (ETDEWEB)

    Beiki, D. [Research Inst. for Nuclear Medicine, Tehran Univ. of Medical Sciences, Tehran (Iran); Dept. of Nuclear Pharmacy, Tehran Univ. of Medical Sciences, Tehran (Iran); Fallahi, B.; Fard-Esfahani, A.; Eftekhari, M. [Research Inst. for Nuclear Medicine, Tehran Univ. of Medical Sciences, Tehran (Iran); Mohseni, Z.; Khalaj, A. [Dept. of Nuclear Pharmacy, Tehran Univ. of Medical Sciences, Tehran (Iran)

    2010-07-01

    Some studies reported that {sup 99m}Tc-MIBI may redistribute in ischaemic myocardium and this phenomenon may have potential role for better assessment of viability by delayed {sup 99m}Tc-MIBI imaging. Some studies also suggested that infusion of low dose dobutamine during delayed imaging may enhance the value of {sup 99m}Tc-MIBI imaging for evaluation of viability. The aim of this study is to determine whether the observed changes of perfusion defects on delayed images are caused by early radiotracer redistribution or as a result of reversal partial volume effect secondary to inotropic stimulation. Patients, methods: 89 patients with angiographically proven coronary artery disease (CAD) were enrolled in this randomized clinical trial study. In all cases, gated-SPECT images were obtained 60 minutes after stress with dipyridamole injection. Subsequently the patients were randomly allocated in two groups and the second imaging was performed at 120{sup th} minute during low dose dobutamine (dobutamine group; 45 cases) or placebo infusion (placebo group; 44 cases). Difference between summed stress score of the first (SSS{sub 1}) and second (SSS{sub 2}) stress images ({delta}SSS) was considered as a marker of reversibility in single-injection double-acquisition (SIDA) protocol. Also summed difference score (SDS) was recorded as a marker of reversibility in standard stress/rest, double-injection double-acquisition (DIDA) protocol. {delta}SSS of the two studied groups were compared. Also the correlation and agreement between {delta}SSS and SDS were analyzed. Results: A significant difference was found between SSS{sub 1} (median 15, range 0-48) and SSS{sub 2} (median 11, range 0-42) in total patients (p < 0.0001). A significant correlation was noted between {delta}SSS and SDS in dobutamine group (r = 0.58, p = 0.002) as well as in placebo group (r = 0.57, p < 0.0001). Considering DIDA protocol as a standard reference method, the influence of dobutamine infusion was not

  7. Value of I-123-subtraction and single-photon emission computed tomography in addition to planar Tc-99m-MIBI scintigraphy before parathyroid surgery

    NARCIS (Netherlands)

    Jorna, Francisca H.; Jager, Pieter L.; Que, Tjin H.; Lemstra, Clara; Plukker, John T. M.

    2007-01-01

    Purpose. To find out if single-photon emission computed tomography (SPECT) and I-123-subtraction can enhance the findings of Tc-99-methoxyisobutylisonitrile (MIBI) scintigraphy for the preoperative localization of parathyroid (PT) tumors. Methods. Among the 111 consecutive patients who underwent pre

  8. Clinical role of {sup 99m}TcO{sub 4}/MIBI scan, ultrasound and intra-operative gamma probe in the performance of unilateral and minimally invasive surgery in primary hyperparathyroidism

    Energy Technology Data Exchange (ETDEWEB)

    Casara, D.; Rubello, D. [Dept. of Radiotherapy, Regional Hospital of Padova (Italy); Pelizzo, M.R. [Dept. of Surgery, University of Padova, Padova (Italy); Shapiro, B. [Dept. of Radiology, University of Michigan Medical Center, Ann Arbor (United States)

    2001-09-01

    One hundred and forty-three consecutive patients with primary HPT were enrolled in the study. We used a modified {sup 99m}TcO{sub 4}/MIBI scintigraphic procedure which included the oral administration of potassium perchlorate to cause rapid {sup 99m}TcO{sub 4} washout from the thyroid tissue, thereby permitting the acquisition of high-quality early MIBI images. A single-photon emission tomography (SPET) acquisition was also obtained in 21 patients, of whom seven had an enlarged parathyroid gland (EPG) in the mediastinum at planar scintigraphy and 14 had discordant scan/US findings for the presence of a cervical EPG. Neck US was performed in the same session as scintigraphy using a small-parts, high-resolution 10-MHz transducer. All patients were then operated on by the same surgical team. Quick PTH assay (QPTH) was used to measure PTH intraoperatively to confirm successful parathyroidectomy. In patients with scan/US evidence of a solitary EPG and with a normal thyroid gland, limited, unilateral neck surgery or, more recently, MIRS was planned (n=91). In patients with scan/US evidence of multiglandular disease (MGD) (n=21) or concomitant nodular goitre (n=24) or in patients with a negative scan/US evaluation (n=7), extensive bilateral neck exploration was planned (n=52). In 87 of the 91 patients (95.6%) in whom preoperative imaging indicated the presence of a solitary EPG and a normal thyroid gland, a single parathyroid adenoma was found at surgery, and these patients were treated by unilateral neck exploration or MIRS. In the remaining four patients of this group, conversion to bilateral neck exploration was required because parathyroid carcinoma (n=3) or MGD (n=1) was diagnosed at operation. In some cases SPET was helpful in better localising the EPG. In particular, in 5 of the 21 patients evaluated, SPET localised an EPG deep in the neck or mediastinum and at surgery a parathyroid adenoma was found in the paratracheal or para-oesophageal space. In 43 of the 46

  9. Evaluation of 99m Tc-MIBI myocardial perfusion imaging with intravenous infusion of adenosine triphosphate in diagnosis of coronary artery disease%静脉注射三磷酸腺苷99mTc-MIBI心肌灌注显像诊断冠心病的评价

    Institute of Scientific and Technical Information of China (English)

    何青; 姚稚明; 于雪; 屈婉莹; 孙福成; 季福绥; 许锋; 钱贻简

    2002-01-01

    目的评价三磷酸腺苷(ATP)药物负荷99mTc-MIBI 心肌灌注断层显像在诊断冠心病中的可行性、安全性和特异性.方法共263例临床诊断为冠心病的病人.所有病人都行ATP负荷的99mTc-MIBI心肌灌注显像(0.16 mg/kg/min, 5 min)检查. 在静脉注射ATP 3分钟时静脉注射20 mCi的99mTc-MIBI, 60分钟后行心肌断层显像.再于48小时后静脉注射99mTc-MIBI 20 mCi, 行静息心肌灌注断层显像.51例病人在2周内行冠状动脉造影以评价ATP介入心肌灌注断层显像诊断冠心病的准确性.在静脉注射ATP的过程中仔细地观察心脏的和非心脏的反应.结果所有病人都完成整个ATP负荷试验.尽管有58.9% 的病人有不同类型的副作用发生,但其程度都不严重.无任何病人需要氨茶碱.最为严重的副作用是II度II型房室传导阻滞(4/263 ),但其持续时间均短暂.ATP介入心肌灌注断层显像诊断冠心病的敏感性和特异性分别为97.1%和82.4%.结论对于不能完成运动试验的病人,ATP负荷心肌灌注断层显像是安全、可行的诊断冠心病的影像学技术.%Objective To evaluate the feasibility, safety and diagnostic accuracy of pharmacologic stress of 99m Technetium-MIBI single-photon emission computed tomography (SPECT) with intravenous adenosine triphosphate (ATP) in patients with suspected coronary artery disease.Methods The study group included 263 patients who were suspected of having coronary artery disease. All patients underwent 99m Tc-MIBI myocardial perfusion imaging with ATP infusion (0.16 mg/kg body weight per min for 5 min). 20 mCi of 99m Tc-MIBI were injected 3 minutes after the start of ATP infusion. Myocardial SPECT images were obtained 60 minutes later. Then, two days later, 20 mCi of 99m Tc-MIBI were administered at rest and myocardial SPECT was repeated. 51 patients also underwent coronary angiography within two weeks for evaluation of sensitivity and specificity of ATP-myocardial perfusion

  10. 99mTc-MIBI评价2-脱氧-D-葡萄糖对鼻咽癌耐药株多药耐药的逆转作用及机制%Evaluation of reversal effect of 2-DG on multidrug resistance by detecting uptake of 99mTc-MIBI in HNE-1/DDP cells

    Institute of Scientific and Technical Information of China (English)

    申勇; 孙伟莉; 袁超; 徐慧琴; 刘斌

    2015-01-01

    Aim To evaluate the reversal effect of 2-deoxy-D-glucose ( 2-DG ) on multidrug resistance ( MDR) by observing the uptake change of 99m Tc-MIBI in HNE-1/DDP cells, and to explore its mechanism. Methods The uptake of 99m Tc-MIBI in HNE-1/DDP cells under different concentrations of 2-DG was detec-ted by γ-counter, and the clearance rates of 99m Tc-MI-BI in HNE-1 cells and HNE-1/DDP cells after treated with 2-DG (10 mmol·L-1 ) were compared. The con-tent of ATP in HNE-1/DDP cells was detected after treated with 2-DG. P-glycoprotein ( P-gp ) and multi-drug resistance-associated proteins ( MRP ) expression were measured by Western blot. Apoptotic HNE-1/DDP cells treated with DDP alone or combined with 2-DG (10 mmol·L-1 ) were detected by propidium io-dide ( PI ) staining. Results The clearance rate of 99m Tc-MIBI in HNE-1/DDP cells was 54. 8%, which was significantly higher than that ( - 41. 3%) in HNE-1 cells (P<0. 01). The clearance rate of 99mTc-MIBI in HNE-1/DDP cells was -203. 7% after treat-ment with 2-DG ( 10 mmol · L-1 ) , which could be significantly reduced compared with the control group ( P<0. 01 ) . The level of ATP was 55 . 69% compared with the negative control group and the expression of P-gp and MRP protein decreased dramatically in HNE-1/DDP. With the combination of 2-DG and DDP, the ap-optotic rate of HNE-1/DDP cells reached 49 . 4%which was significantly higher than DDP treated group (22. 5%) . Conclusion Multidrug resistance and the reversal effect of 2-DG on multidrug resistance could be evaluated effectively by detecting the uptake change of 99m Tc-MIBI in HNE-1/DDP cells. The mechanism may be related with the inhibition of ATP level and the re-duced expression of P-gp and MRP protein in cancer cells.%目的:探讨2-脱氧-D-葡萄糖(2-DG)对鼻咽癌顺铂耐药株细胞( HNE-1/DDP )多药耐药( MDR )的逆转机制,观察99m锝-甲氧基异丁基异腈(99m technetium-methoxyisobutyli-sonitrile,99m Tc-MIBI)细胞内的摄取变化,评价2

  11. Evaluation of occult lymph node metastases by means of 99mTc-MIBI scintigraphy and sentinel node biopsy with RT-PCR multi-marker assay in early-stage melanoma patients

    International Nuclear Information System (INIS)

    Early diagnosis of regional lymph node metastasis is a key step in the management of melanoma patients. This enables the identification of those patients who are candidates for adjuvant therapy and early therapeutic lymph node dissection. PET using FDG, has been shown to be effective in detecting recurrent melanoma lesions. However, this technique has no proved to offer any significant advantage in diagnostic sensitivity for pre-treatment nodal staging. Lymphoscintigraphy along with intraoperative probe detection of the sentinel node (SN) has become widely accepted for regional lymph node staging. Serial sectioning and immuno-histochemistry (IHC) have been shown to improve the detection of occult melanoma cells, compared with conventional hematoxylin and eosin (H and E) staining alone. More recently, mRNA multi-marker RT-PCR assays have been used to detect micro metastases in lymph nodes where disease was not found by either H and E or IHC techniques. During the last few years, our group has been studying the clinical value of 99mTc-MIBI scintigraphy for the evaluation of melanoma lesions. The technique was found to have the potential of detecting sub clinical recurrent disease including lymph node metastases. Therefore, the aim of the study was to compare the sensitivity of 99mTc-MIBI with that of sentinel node biopsy (SNB) with histopathological and molecular analysis, for the detection of sub clinical nodal disease

  12. Normative values of 99mTc-MIBI distribution in myocardium in males and females, as a basis for quantitative planar scintigraphic method for detection of coronary artery disease in patients of both sexes

    International Nuclear Information System (INIS)

    Radionuclide perfusion studies of myocardium are being performed using planar and tomographic (SPECT) procedures. The latter method enables better detection as well as assessment of localisation and severity of scintigraphically visualised perfusion defects. On the other hand, lower cost of planar procedures using 99mTc-MIBI as the tracer and their much wider availability in countries of Central and Eastern Europe could significantly increase the diagnostic potential of nuclear cardiology in this region.The aim of the study was an assessment of normal distribution of 99mTc-MIBI in the myocardium and generation of normative basis for quantitative planar scintigraphic procedure, aiming at detection of CAD in patients of both sexes.The study was based on 250 patients. The reference (control) group consisted of 53 individuals (29 men, 24 women) with the low (0 and LAO 700). These mean curves differed significantly between both sexes. In LAO 450 projection the differences affected mostly the region of the septum and postero-lateral wall of the left ventricle (LV). In LAO 700 projection differences were most pronounced in the antero-septal wall of the LV. Sensitivity, specificity and accuracy of CAD detection using the elaborated method, taking into account inter-sex differences, amounted to 86, 87 and 86% respectively in males, and correspondingly 81, 84 and 83% in females. The differences between corresponding indices for two sexes were statistically insignificant. For the whole group of patients the sensitivity, specificity and accuracy was 85, 86 and 85%, respectively. (author)

  13. Comparative scanning of thyroid nodules with technetium-99m pertechnetate and technetium-99m methoxyisobutylisonitrile

    Energy Technology Data Exchange (ETDEWEB)

    Foeldes, I.; Levay, A. (Central Hospital of the Ministry of the Interior, Budapest (Hungary). Dept. of Nuclear Medicine); Stotz, G. (Central Hospital of the Ministry of the Interior, Budapest (Hungary). Dept. of Pathology)

    1993-04-01

    Thyroid imaging was performed using technetium-99m methoxyisobutylisonitrile and technetium-99m pertechnetate in 58 patients. The [sup 99m]Tc-pertechnetate scans showed a total of 77 nodules: 60 cold, 13 hot and 4 of normal acitivity. There was no [sup 99m]Tc-MIBI accumulation in 46.4% of [sup 99m]TC-pertechnetate cold nodules; 27 (45%) of these nodules showed [sup 99m]TC-MIBI uptake with the same intensity as the surrounding normal tissue, and five (8.6%) became hot with [sup 99m]Tc-MIBI. Of the [sup 99m]TC-pertechnetate hot nodules 11 (84.6%) could not be differentiated from the normal extranodular tissue on the [sup 99m]Tc-MIBI scan. The histopathology of 34 surgically removed nodules proved that increased, normal or decreased [sup 99m]Tc-MIBI accumulation is not specific for thyroid malignance and that the [sup 99m]Tc-MIBI uptake depends mainly on the viability of thyroid tissue. (orig.).

  14. Technetium-99m Sestamibi in Multiple Myeloma

    International Nuclear Information System (INIS)

    Technetium-99m 2-methoxy - isobutyl - isonitrile (99mTc-MIBI) has been reported to be useful in evaluating patients with multiple myeloma. The aim of this study is to evaluate the role of technetium-99m sestamibi (99mTc-MIBI) scintigraphy in the diagnosis. staging and follow-up of patients with multiple myeloma. Methods and Materials: twenty-five consecutive patients with multiple myeloma were studied using 99mTc- MIBI. Of the 25 patients included in this study, 6 were in stage I, II in stage II and 8 in stage III. Anterior and posterior whole-body imaging were obtained 20 min after I.V. injection of 740 MBq of 99mTc-MIBI. Four different MIBI patterns could be described in our patients: physiological (P), diffuse (D), focal (F) and combined diffuse and focal (D+F). All patients in stages II and III as well as 3 patients in stage I were treated with chemotherapy (cyclophosphamide and prednisone) then 99mTc-MlBI scans were repeated after 6 courses. Results: in comparison to conventional X-ray skeletal survey, 99mTc-MIBI scans showed a higher number of myeloma bone disease at diagnosis. All patients with stage II and III multiple myeloma were positive with 99mTc-MlBl scans at diagnosis. The pattern of positive MIBI accumulation was diffuse in 13 (52%) patients, focal in 4 (16%) and combined focal and diffuse in 6 (24%) patients. The intensity of 99mTc-MIBI correlated with disease activity as determined by lactate dehydrogenase (LDH), number of plasma cells in bone marrow and serum electrophoresis. There was a direct correlation between 99mTc-MIBI scan result and clinical outcome of patients following 6 courses of chemotherapy. Sensitivity and specificity of 99mTc-MIBI scintigraphy in detecting myeloma bone lesions were 92% and 90% respectively. Conclusion: 99mTc-MIBI scintigraphy is a reliable method to evaluate bone marrow activity in patients with multiple myeloma and follow-up of myeloma bone lesions

  15. Surgical management of primary hyperparathyroidism guided by double-phase Tc-99m-MIBI scintigraphy%Tc-99m-MIBI双时相显像在原发性甲状旁腺功能亢进症手术治疗中的价值

    Institute of Scientific and Technical Information of China (English)

    Abdel Hamid Hussein Ezzat; Tarek El. Baradie; Amr Attia; Magdy Kotb; Ahmad Zaher; Iman Gouda

    2012-01-01

    Objective: The aim of this work was to study the clinicopathological features of cases with primary hyperparathyroidism (PHPT) referred to National Cancer Institute (NCI), Cairo University in the last six years and to study the role of nuclear medicine techniques in the initial diagnosis and preoperative localization directing surgical management of these cases. Methods: This study included 27 patients with PHPT properly diagnosed and treated in the NCI, Cairo University from January 2005 to December 2010. Preoperative neck U/S and Tc-99m-MIBI scintigraphy were done in all cases. If preoperative localization detected a single lesion, unilateral exploration was done. Bilateral exploration was done if multiple foci of active parathyroid glands or no lesions were detected. Results: This study included 27 patients (8 males and 19 females). The median age was 43 years (range from 19 to 68 years). All cases presented with bone disease in the form of bony pain in 23 patients, bony swellings in 15 patients and pathological fractures in 6 cases. Twenty one patients (77.8%) had single adenoma, 5 cases (18.5%) had parathyroid hyperplasia, and only one case had double adenomas. The sensitivity of neck U/S in detecting single adenoma was 61.9% (13/21), with 81% positive predictive value (PPV) while the sensitivity of Tc-99m- MIBI scintigraphy was 90.5% (19/21) with 100% PPV. Based on preoperative localization tests, unilateral exploration was done in 19 patients with solitary adenomas. All cases in this group were cured with no reported case of persistent or recurrent hypercalcemia. Conclusion: Presentation of PHPT may mimic malignant bone tumors but fortunately these patients were correctly diagnosed with bone scan which confirm the presence of metabolic bone disease rather than bone metastases. This was followed by estimation of serum calcium and parathormone levels for confirmation. Unilateral exploration based on the combination of ultrasound and preoperative localization

  16. {sup 99m}Tc-M.I.B.I. pinhole SPECT in primary hyperparathyroidism; Tomographie stenopeique au {sup 99m}Tc-MIBI dans l'hyperparathyroidie primaire

    Energy Technology Data Exchange (ETDEWEB)

    Oudoux, A.; Carlier, T.; Bodet-Milin, C.; Kraeber-Bodere, F.; Ansquer, C. [Centre Hospitalier Universitaire, Service de Medecine Nucleaire, Hotel-Dieu, 44 - Nantes (France); Mirallie, E. [Centre Hospitalier Universitaire, Service de Chirurgie, Hotel-Dieu, 44 - Nantes (France); Seret, A. [Liege Univ. (Belgium). Inst. de Physique; Aubron, F.; Daumy, I. [Centre d' Echographie de l' ile Gloriette, 44 - Nantes (France); Leux, C. [Centre Hospitalier Universitaire, PIMESP, Hopital Saint-Jacques, 44 - Nantes (France)

    2007-10-15

    Purpose: this prospective study was conducted to determine the interest of 99 mTc-M.I.B.I. pinhole SPECT compared with conventional SPECT, planar scintigraphy and ultrasonography, for the preoperative localization of parathyroid lesions in primary hyperparathyroidism. Methods: fifty-one patients cured after surgery were studied. Pinhole SPECT was reconstructed with a dedicated O.S.E.M. algorithm. Scintigraphies were analyzed visually. A diagnostic confidence score (C.S.) was assigned to each procedure considering intensity and extra thyroidal location of suspected lesions and was defined as follows: 0 = negative, 1 = doubtful, 2 = moderately positive, 3 positive. Results: surgery revealed 55 lesions. Sensitivity of ultrasonography, planar imaging, conventional SPECT and pinhole SPECT were respectively, 51, 76, 82 and 87%. Five glands were only detected by pinhole SPECT. Combination of ultrasonography, planar and pinhole SPECT showed the highest sensitivity (94.5%). The mean C.S. of the 55 pathologic glands was significantly higher with pinhole SPECT compared with planar imaging and conventional SPECT (p < 0.0001). Compared with planar imaging and conventional SPECT, pinhole SPECT increased C.S. for 42 and 53% of parathyroid lesions, respectively, and contributed to markedly reduce the number of uncertain results. Nevertheless, planar imaging and ultrasonography were useful to analyze thyroid morphology and to detect some ectopic glands. Conclusion: the use of pinhole SPECT increases sensitivity and C.S. of scintigraphy. Combination of ultrasonography, planar and pinhole SPECT appears the optimal preoperative imaging procedure in primary hyperparathyroidism. (authors)

  17. Comparison study among methodologies of planar chromatography for radiochemical control of technetium-99m; Estudo comparativo entre metodologias de cromatografia planar para controle radioquimico de radiofarmacos de tecnecio-99m

    Energy Technology Data Exchange (ETDEWEB)

    Monteiro, Elisiane de Godoy

    2012-07-01

    Radiopharmaceuticals are substances that have radioisotopes in their composition. About 95% of the procedures performed in nuclear medicine use radiopharmaceuticals with diagnostic purposes, and the Lyophilized Reagents (LR) labeled with Technetium-99m ({sup 99}mTc), obtained from {sup 99}Mo/{sup 99}mTc generator, are the most one used. Quality Control represents the set of assays to be performed to assure that the product is adequate to its purpose. An important feature to be evaluated in {sup 99m}Tc radiopharmaceuticals is the radiochemical purity (% RqP) to quantify free pertechnetate ({sup 99}mTcO{sub 4}{sup -}) and technetium colloidal (99mTcO{sub 2}) mainly by paper chromatography (PC), thin layer (TLC) and High Performance Liquid Chromatography (HPLC). The objective of this work was to perform the comparison among the radiochemical control methodologies of LR labeled with {sup 99m}Tc, described in the United States Pharmacopoeia (USP) and European Pharmacopoeia (EP) and those used by IPEN. {sup 99m}TcO{sub 4}{sup -} eluate and DISIDA, DMSA, DTPA, EC, ECD, GHA, MIBI, MDP, PIRO, SAH and Sn Coloidal LR were provided by IPEN-CNEN/SP. TLC-cellulose, TLC-SG.TLC-SG reverse phase, HPTLC-cellulose, HPTLC-SG (Merck) and ITLC-SG (Pall Corporation), W1MM, W3MM, W17M e W31ET (Whatman) chromatographic plates were used. The measurement of the radioactivity was done in a Perkin Elmer Cobra D-5002 gamma counter. LR were labeled to obtain 55,0 MBq mL{sup 1} (1,5 mCi mL{sup 1}) of final radioactive concentration. The %{sup 99m}TcO{sub 4}{sup -}, %{sup 99m}TcO{sub 2} and % RqP were determined up to 4 hour labeling. From 11 LR, only EC and GHA have no radiochemical control methods in USP and EP. In USP and/or EP, DTPA, MDP, PIRO, SAH and Sn Coloidal methods use ITLC-SG; IPEN uses this chromatography plate in DISIDA, EC, ECD, GHA, PIRO, MIBI and SAH. As ITLC-SG had been out of production (recommended in 40, 70 and 41% of the USP, EP and IPEN methodologies, respectively), it was

  18. Nude mice multi-drug resistance model of orthotopic transplantation of liver neoplasm and Tc-99m MIBI SPECT on p-glycoprotein

    Institute of Scientific and Technical Information of China (English)

    Yu Han; Xiao-Ping Chen; Zhi-Yong Huang; Hong Zhu

    2005-01-01

    AIM: To establish a model of drug-resistant neoplasms using a nude mice model, orthotopic transplantation of liver neoplasm and sporadic abdominal chemotherapy.METHODS: Hepatocellular carcinoma cells HepG2 were cultured and injected subdermally to form the tumorsupplying mice. The orthotopic drug-resistant tumors were formed by implanting the tumor bits under the envelope of the mice liver and induced by abdominal chemotherapy with Pharmorubicin. Physical examination, ultrasonography, spiral CT and visual inspection were used to examine tumor progression. RT-PCR and immunohistochemistry wereused to detect expression of mdr1 mRNA and its encodedprotein p-glycoprotein (p-gp). Tc-99m sestamibi scintigraphy was performed by obtaining planar abdominal images at 20 min after injection, and the liver/heart ratios werecalculated.RESULTS: Post-implantation mortality was 0% (0/25),tumor implantation success was 90% (22/25), and the rate of implanting successfully for the second time was 100% (3/3). Tumor induction using Pharmorubicin was 80% (16/20). The mdr1 mRNA expression of the induced group was 23 times higher than that of the control group, and p-gp protein expression was 13-fold higher compared to the control group. The liver/heart ratio (as assessed in vivo, using Tc-99m radiography) was decreased significantly in the induced group as compared to the control group. CONCLUSION: We have established an in vivo model of mdr1 in nude mice by orthotopic transplantation of liver neoplasm coupled to chemotherapy. We propose that identification of drug resistance as characterized by decreased 99mTc-ppm radiography due to enhanced clearance by p-gp may be useful in detecting in vivo drug resistance, as well as a useful tool in designing more effective therapies.

  19. Clinical Value of Double-phase 99mTc-MIBI Scintigraphy in the Diagnosis of Hyperparathyroidism%99mTc-MIBI双时相法诊断甲状旁腺功能亢进症的临床价值

    Institute of Scientific and Technical Information of China (English)

    唐玲; 马云川; 王荣福; 付占立; 范岩

    2008-01-01

    目的 探讨核素99mTc-甲氧基异丁基异腈(99mTc-MIBI)双时相法显像在诊断甲状旁腺功能亢进症(hyper-parathyroidism,HPT)中的作用,并与其他影像检查方法进行比较.方法 65例临床考虑为HPT的患者均分别进行了核素显像及超声检查, 20例做了CT检查,11例做了MRI检查. 核素显像采用99mTc-MIBI双时相法.比较几种影像检查的灵敏度、特异性,分析其对HPT的诊断价值.结果 65例患者中,甲状旁腺激素(PTH)增高36例,以PTH结果为诊断标准.核素检查阳性34例,阴性2例,灵敏度94.4%,特异性100%,准确性96.9%;超声检出32例,阴性4例,假阳性2例,其灵敏度88.9%,特异性93.1%,准确性90.8%;CT阳性17例,MRI阳性8例.结论 核素99mTc-MIBI双时相法甲状旁腺显像是一种简便有效的检查方法,可提高甲状旁腺瘤术前定位诊断的准确率.临床高度怀疑原发性甲状旁腺功能亢进症的病人,若超声检查阴性应进一步行核素检查除外甲状旁腺瘤.

  20. 99mTc-MIBISPECT-CT显像在纵隔内异位甲状旁腺诊治中的应用价值%Application value of 99m Tc-MIBI SPECT-CT scintigraphy in diagnosis and treatment of mediastinal ectopic parathyroid

    Institute of Scientific and Technical Information of China (English)

    李征

    2015-01-01

    Objective To analyze the application value of single photon emission computed tomography (SPECT)‐CT 99m Tc‐methoxyisobutylisonitrile(MIBI) scintigraphy in the diagnosis and treatment of mediastinal ec‐topic parathyroid .Methods 100 cases of secondary hyperparathyroidism in the Beijing Shijitan Hospital from Aug . 2013 to Feb .2013 were selected as the research subjects and performed the 99m Tc‐MIBI dual‐phase scintigraphy , plane and SPECT and CT imaging with the same machine .The fused images of mediastinal abnormal radioactive high uptake lesions were analyzed .And the relationship between T/NT radioactivity ratio and intact parathyroid hormone (iPTH) level and pathological results were also analyzed .Results Among 100 cases ,mediastinal ectopic parathyroid appeared in 2 cases (2 .0% ) ,in which 1 case was given the parathyroidectomy before imaging and the postoperative iPTH was increased again;another case was treated by medication .These two cases showed the ectopic parathyroid imaging by postoperative SPECT‐CT imaging ,which located in the sternoclavicular joint .The plane ,SPECT and CT imaging with the same machine showed that the highest T/NT radioactivity ratio of parathyroid was positively corre‐lated with the iPT H level before imagine(r=0 .419 ,P0 .05) .Conclusion 99m Tc‐MIBI SPECT‐CT scintigraphy could effectively fuse the anatomical im‐ages and functional images ,may have the extremely high specificity ,sensitivity and accuracy for the diagnosis of hy‐perparathyroidism ,at the same time could accurately localize the lesions ,increase the operation efficiency and make the operation to reach the satisfactory effect .%目的:分析99mTc‐甲氧基异丁基异腈(MIBI)单光子发射计算机断层显像(SPECT)‐CT显像在纵隔内异位甲状旁腺诊治中的应用价值。方法以2013年8月至2014年2月北京世纪坛医院收治的100例继发性甲状旁腺功能亢进症(SHPT)患者为研究

  1. Study on Effect of Shenmai Injection Protecting Myocardium against Ischemia-Reperfusion Injury in Thrombolytic Therapy with Urokinase for Acute Myocardial Infarction Patient Evaluated by 99mTc-MIBI Myocardial Imaging

    Institute of Scientific and Technical Information of China (English)

    郭松鹏; 张言镇

    2001-01-01

    Objective: To evaluate the myocardial protecting effect of Shenmai injection (SMI) against ischemia/reperfusion injury in thrombolytic therapy with urokinase (UK) for acute myocardial infarction patients by 99mTc-MIBI myocardial imaging (SPECT). Methods: Five hundred and thirty-seven patients were divided into two groups randomly. The SMI group (n=292) was treated with thrombolytictreatment plus SMI and the control group (n=245) with thrombolytic treatment solely. Single photon emission computed tomography (SPECT) was carried out on the 7th day after thrombolysis to determine the ischemic myocardial area (IMA) and ejection fraction (EF) in both groups and compared. Results: The infarction related area (IRA) of reperfusion rate in the two groups was not different significantly (72.26% vs 72.65%, P >0.05). The IMA in patients of the SMI group, no matter with or without reperfused IRA (211 cases and 81 cases) respectively, was significantly lower than that in the control group (178 cases and 67 cases) respectively, P<0.01 and P<0.05 respectively. The EF value in the SMI group was significantly higher than that in the control group (P<0.01). Conclusion:Using SMI in early stage of thrombolytic treatment in acute myocardial infarction could significantly reduce IMA and increase EF. SMI showed good protective effect against myocardial ischemia/reperfusion injury in thrombolytic treatment.

  2. Study on Effect of Shenmai Injection Protecting Myocardium against Ischemia-Reperfusion Injury in Thrombolytic Therapy with Urokinase for Acute Myocardial Infarction Patient Evaluated by 99mTc-MIBI Myocardial Imaging

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective: To evaluate the myocardial protecting effect of Shenmai injection (SMI) against ischemia/reperfusion injury in thrombolytic therapy with urokinase (UK) for acute myocardial infarction patients by 99mTc-MIBI myocardial imaging (SPECT). Methods: Five hundred and thirty-seven patients were divided into two groups randomly. The SMI group (n=292) was treated with thrombolytictreatment plus SMI and the control group (n=245) with thrombolytic treatment solely. Single photon emission computed tomography (SPECT) was carried out on the 7th day after thrombolysis to determine the ischemic myocardial area (IMA) and ejection fraction (EF) in both groups and compared. Results: The infarction related area (IRA) of reperfusion rate in the two groups was not different significantly (72.26% vs 72.65%, P >0.05). The IMA in patients of the SMI group, no matter with or without reperfused IRA (211 cases and 81 cases) respectively, was significantly lower than that in the control group (178 cases and 67 cases) respectively, P<0.01 and P<0.05 respectively. The EF value in the SMI group was significantly higher than that in the control group (P<0.01). Conclusion:Using SMI in early stage of thrombolytic treatment in acute myocardial infarction could significantly reduce IMA and increase EF. SMI showed good protective effect against myocardial ischemia/reperfusion injury in thrombolytic treatment.

  3. Marcaje de ciprofloxacina con 99mTc para el diagnóstico de infección activa.

    Directory of Open Access Journals (Sweden)

    Abel Hernández Cairo

    2005-01-01

    Full Text Available Introducción: La 99mTc-Ciprofloxacina, es un radiofármaco eficaz para el diagnóstico gammagráfico de infecciones que sólo detecta la presencia de bacterias vivas, ventaja que la distingue de los productos ya conocidos. Radiomarcarla con 99mTc, estudiar su actividad biológica in vivo y su biodistribución en ratas, fueron los objetivos propuestos en esta investigación. Materiales y Métodos: Para marcar con 99mTc se estudiaron varias relaciones molares Ciprofloxacina: Fluoruro de Estaño (SnF2, manteniendo fija la cantidad de fármaco y la actividad. Se controló la pureza por cromatografía en TLC silica gel. Se realizó modelo de absceso con Staphilococcus Aureus (SA y Escherichia coli (EC y los estudios biocinéticos se realizaron en ratas Wistar. Se administró 666-740 MBq/kg de 99mTc-Ciprofloxacina y se midió el porciento de dosis inyectada en diferentes órganos. Se colectó toda la orina durante 24 horas en 4 ratas. Resultados: La mayor pureza radioquímica se obtuvo cuando se utilizó 19.1¿g del reductor (92.5 ± 2.5%. El marcaje fue >90% por más de 10 horas. La relación lesión/ fondo en el modelo animal se mantuvo en aumento durante 20 horas. El momento óptimo para la adquisición resulto ser a las 4 horas de la inyección del radiofármaco. La biodistribución mostró marcada acumulación en hígado, riñones y en intestinos todo el tiempo. Por vía renal se eliminó el 41±1% de la dosis. Conclusiones: Con esta técnica se logró un alto rendimiento de 99mTc-Ciprofloxacina con adecuada estabilidad. El radiofármaco fue capaz de reconocer abscesos activos en zonas músculo-esqueléticas de ratas Wistar y presentó una biocinética favorable para los propósitos perseguidos en la clínica. La técnica es simple y perfectamente reproducible.

  4. 99mTc-MIBI运动后早期门控心肌显像诊断冠心病的价值%Diagnostic value of ECG-gated early post-exercise 99mTc-MIBI myocardial perfusion imaging in coronary artery disease

    Institute of Scientific and Technical Information of China (English)

    李殿富; 黄峻; 冯建林; 李建华; 程旭; 杨志建; 朱铁兵; 曹克将

    2006-01-01

    目的:比较99mTc-MIBI门控心肌显像(G-MPI)与传统的非门控心肌显像(NG-MPI)对冠心病(CHD)的诊断价值.方法:215例同期1个月内做负荷(运动后15~20 min)/静息2日法99mTc-MIBI G-MPI和冠状动脉造影的CHD患者,以冠状动脉直径狭窄≥50%为诊断标准,比较NG-MPI和G-MPI的诊断价值. 结果:心电图运动试验(ETT)、NG-MPI和G-MPI诊断CHD的敏感性分别为:58.0%,88.3%,90.7%;特异性分别为:67.9%,79.2%,88.7%.NG-MPI和G-MPI的敏感性与ETT相比均差异有统计学意义(P<0.01).G-MPI的诊断敏感性比NG-MPI有增高趋势 ,但两者比较差异无统计学意义(P>0.05).在诊断特异性方面,G-MPI与ETT相比差异有统计学意义 (P<0.01);对男、女2个性别的特异性分析发现,G-MPI对女性诊断特异性的增加尤其显著.对于严重CHD(狭窄≥70%), G-MPI对严重3支病变的诊断敏感性显著高于NG-MPI(100%:92.2%,P<0.05).结论:G-MPI对诊断CHD的价值显著优越于ETT,也优于NG-MPI.

  5. 99mTc-sestamibi and 99mTc-tetrofosmin scintigraphy in myeloma bone disease

    International Nuclear Information System (INIS)

    Full text: Evaluation of the role of scintigraphy with 99mTc-Sestamibi (99mTc-MIBI) and 99mTc-Tetrofosmin (99mTc-TF) in the detection of bone marrow involvement in patients (pts) with multiple myeloma (MM) and in follow-up. 62 pts with MM and 38 pts with monoclonal gammopathy of undetermined significance (MGUS) were enrolled in this study. Forty-seven out sixty-two MM pts had active disease (AD), 10/62 were in complete remission (CR) and 5/62 in partial remission (PR) after chemotherapy. Whole-body scans were obtained 10 min after the i.v. injection of 740 MBq of 99mTc-MIBI in anterior and posterior view. The scans were scored semiquantitatively according to extension and intensity of tracer uptake. All 38 MGUS pts had a negative 99mTc-MIBI scan. As to the MM pts, 49/62 pts (44 with AD, 4 with PR and 1 with CR) had a positive 99mTc-MIBI scan, while the 99mTc-MIBI scan was negative in 13/62 pts (9 with CR, 1 with PR and 3 with AD). The overall sensitivity of the scintigraphic procedure was 92 % while specificity was 97 %. A total of 42 follow-up scans with 99mTc-MIBI were performed in 30 MM pts after high-dose chemotherapy, with an average follow-up duration of 14.6 ± 8.9. The follow-up 99mTc-MIBI scintigraphy was positive in 14/15 of the AD pts, while 6/8 negative scans were observed in the CR pts; the seven PR pts exhibited in five cases a negative scan and in two a mild scintigrafic positivity. The overall sensitivity and specificity of the follow-up scan were, respectively, 86 % and 75 %. In six patients, one at the diagnosis and five showing a positive 99mTc-MIBI scan during the follow-up, 740 MBq of 99mTc-TF was administered within two days with the same acquisition protocol. The scans provided substantially identical information. 99mTc-TF provided a mildly higher contrast between lesion and background activities. The results obtained in this study provide additional evidence indicating that 99mTc-MIBI scintigraphy closely reflects myeloma disease activity in

  6. 131I和99mTc-MIBI全身显像联合血清Tg和CEA检测在分化型甲状腺癌131I治疗随访中的应用%The Application of 131I- and 99mTc-MIBI Whole Body Scan Combined Determination with Serum Thyroglobulin and CEA Levels in the Follow-up of Differentiated Thyroid Carcinoma with131I Radiotherapy

    Institute of Scientific and Technical Information of China (English)

    汪兵; 池晓华; 李贵平; 黄凯; 刘峰; 邓志芳; 黄宝丹

    2011-01-01

    Objective To evaluate the value of131 I- and 99mTc-MBI whole body scan combined determination with serum thyro-globulin and CEA levels in the follow-up of differentiated thyroid carcinoma with I radiotherapy after surgical treatment. Methods Fourty eight patients with DTC after surgical treatment were treated with 131I radiotherapy. 131I-whole body scan combined determination with serum thyroglobulin; CEA levels were performed three to six months after I radiotherapy. While Tc-MIBI whole body scan was performed one year after treatment when 131I-whole body scan was negative. Results Regular follow-up after I treatment in 48 patients showed that 35 cases were found negative with 131I-WBS and serum Tg level while 6 cases were proved positive in both methods; and 5 cases were negative in 131I-WBS with positive serumTg level; on the other hand; 2 cases were positive in I-WBS with negative serum Tg level. 40 cases of 131I-WBS negative patients in the 99mTc-MIBI whole body scintigraphy were also negative. In 48 patients only 2 cases had positive serum CEA level. Conclusion I -whole body scan combined with serum Tg level determination were the most commonly method in the detecting of a recurrent or metastatic thyroid cancer; and 99mTc-MIBI whole body scan and serum CEA level may become a useful supplement to 131I whole body scan and serum Tg level determination.%目的:评价131I-WBS和99mTc-MIBI全身显像联合血清Tg和CEA检测在分化型甲状腺癌术后放射性131I治疗随访中的应用价值.方法:48例DTC患者均行手术治疗并经131I治疗后(3~6)个月随访复查131I-WBS和血清Tg和CEA测定,131I-WBS显像阴性者于治疗后1年行99mTc-MIBI全身显像.结果:48例DTC患者行131I治疗后定期随访显示,35例患者的血清Tg水平及131I全身显象(131I-WBS)均为阴性,6例患者的血清Tg水平和131I-WBS均为阳性,两者符合率为85.42%(41/48);5例患者血清Tg水平为阳性而131I-WBS显像为阴性;2例

  7. Is Brown Adipose Tissue Visualization Reliable on 99mTc-Methoxyisobutylisonitrile Diagnostic SPECT Scintigraphy?

    OpenAIRE

    Haghighatafshar, Mahdi; Farhoudi, Farinaz

    2016-01-01

    Abstract The 99mTc-MIBI has been used with great value as a diagnostic technique in patients with primary hyperparathyroidism. False-positive scans may occur due to misinterpretation of the physiologic distribution of the 99mTc-MIBI. Reviewing consecutive SPECT scans, we evaluated this possibility and assessed how frequently brown adipose tissue (BAT) is seen on 99mTc-MIBI scintigraphy. Here, we retrospectively reviewed scans of consecutive patients who were evaluated for parathyroid adenomas...

  8. 双能CT首过及延迟心肌灌注成像评价心肌活性:与99mT c-MIBI心肌灌注显影的对照研究%First-pass and Delay Myocardial Perfusion Imaging in Assessment of Myocardial Viability Using Dual-energy Computed Tomography:Comparison with99mTc-MIBI Imaging

    Institute of Scientific and Technical Information of China (English)

    李小荣; 欧陕兴; 钱民; 彭光明

    2015-01-01

    目的:通过对心肌梗死患者行双源CT首过及延迟增强双能量扫描,与99mTc-MIBI心肌显像结果进行对比研究,探讨双源CT首过及延迟心肌灌注成像在识别及定位存活心肌的诊断价值。方法28例心肌梗死患者接受双源首过及延迟(5min)心肌灌注扫描及99mTc-MIBI心肌灌注显影检查,观察双源CT心肌灌注碘图评价心肌灌注缺损,将双源CT首过及延迟扫描心肌灌注缺损与99mTc-MIBI的相应节段进行对照。结果28例受检者,共476个心肌节段,双源CT首过扫描心肌灌注共有371节段灌注减低及缺损,其中延迟扫描共156个节段表现为低灌注,215个节段表现为相对高灌注(延迟强化);99mTc-MIBI心肌灌注显影结果共有237心肌灌注减低及缺损;17个节段中,共13节段两者结果一致性好(Kappa值>0.75),其余4节段(第2、3、8、9节段)一致性一般(0.40<Kappa值<0.75);第8节段敏感度最高为(100%),第12节段特异度为最高为(100%),第12节段准确度最高为(92.85%)。结论双源CT首过及延迟心肌灌注成像评价心肌活性与99mTc-MIBI心肌灌注显影对比结果一致性良好,可较为准确地定位心肌梗死,并对心肌活性作出评估。%Objective To evaluate the feasibility and diagnostic accuracy of first-pass and delayed enhancement of dual-energy computed tomography(DECT) in myocardial perfusion imaging for detecting myocardial viability compared with99mTc-MIBI myocardial perfusion imaging.Methods Twenty-eight patients with coronary artery disease were detected by first-pass and delayed enhancement (5min) DECT and99mTc-MIBI PET respectively. A color-coded iodine map was used to evaluate the myocardial perfusion defects. Myocardial perfusion defects according to the first-pass and delayed enhancement were compared with99mTc-MIBI myocardial perfusion imaging on a segmental basis.Results A total of 476 myocardial segments of 28 patients were analyzed

  9. A Pilot Comparison of 18F-fluorocholine PET/CT, Ultrasonography and 123I/99mTc-sestaMIBI Dual-Phase Dual-Isotope Scintigraphy in the Preoperative Localization of Hyperfunctioning Parathyroid Glands in Primary or Secondary Hyperparathyroidism

    OpenAIRE

    Michaud, Laure; Balogova, Sona; Burgess, Alice; Ohnona, Jessica; Huchet, Virginie; Kerrou, Khaldoun; Lefèvre, Marine; Tassart, Marc; Montravers, Françoise; Périé, Sophie; Talbot, Jean-Noël

    2015-01-01

    Abstract We compared 18F-fluorocholine hybrid positron emission tomography/X-ray computed tomography (FCH-PET/CT) with ultrasonography (US) and scintigraphy in patients with hyperparathyroidism and discordant, or equivocal results of US and 123I/99mTc-sesta-methoxyisobutylisonitrile (sestaMIBI) dual-phase parathyroid scintigraphy. FCH-PET/CT was performed in 17 patients with primary (n = 11) lithium induced (n = 1) or secondary hyperparathyroidism (1 dialyzed, 4 renal-transplanted). The refer...

  10. Technetium-99m Sestamibi and [F18]Fluorodeoxyglucose (FDG) PET in assessing patients with multiple myeloma

    International Nuclear Information System (INIS)

    Full text: The aim of the study is to evaluate the diagnostic and prognostic value of 99mTc-Sestamibi (MIBI) in the detection on bone marrow involvement in patients suffering from Multiple Myeloma (MM), its possible role in the follow-up and comparison of 99mTc-MIBI and [F18]FDG PET imaging. We present the results, for patients at our institution, that have undergone 99mTc-MIBI scans to assess MM. We considered patients (pts) with MM (in active disease, partial and chronic remission) and with monoclonal gammopathy of undetermined significance (MGUS). A subgroup of patients with MM repeated a 99mTc-MIBI scintigraphic study at least 2 months after high-dose chemotherapy. All the scans were scored semi-quantitatively according to extension and intensity of tracer uptake. Twelve patients had concurrent 99mTc-MIBI and [F18]FDG PET. The overall sensitivity of the 99mTc-MIBI scintigraphy was 92%, specificity was 96%. In the follow up of the pts treated with chemotherapy 99mTc-sestamibi closely paralleled the activity of myeloma bone disease. A multivariate analysis showed that 99mTc-MIBI uptake pattern has an added value in relation to known prognostic variables. In another study, patients showing disease progression at restaging had higher washout of 99mTc-MIBI (10 and 60 minutes) than patients in remission; disease free survival was significantly better in patients with lower washout in absence of differences in therapeutic regimen and stage of disease at admission. [F18]FDG-PET and MIBI were concordant in seven of 12 cases (58%); only in one case FDG detected bone marrow sites in addition to those detected by 99mTc-MIBI. Our experience indicates that 99mTc-MIBI scintigraphy closely reflects myeloma disease activity in the bone marrow, and that a negative 99mTc-MIBI scan in pts with suspected MM indicates absence of disease or clinical remission. The results of these studies suggest a potential role of 99mTc-MIBI washout in predicting response to chemotherapy in

  11. Are P.T.H. plasma levels useful for the selection of patients with secondary hyperparathyroidism for preoperative MIBI ({sup 99m}Tc)/{sup 123}I dual-isotope scintigraphy?;La concentration plasmatique de PTH permet-elle de selectionner les patients atteints d'hyperparathyroidie secondaire pour beneficier de la scintigraphie double isotope MIBI ({sup 99m}Tc)/{sup 123}I preoperatoire?

    Energy Technology Data Exchange (ETDEWEB)

    Balogova, S.; Sauer, A.M.; Dudczak, J.; Pascal, O.; Kerrou, K.; Huchet, V.; Montravers, F.; Talbot, J.N. [Universite Pierre-et-Marie-Curie, Service de medecine nucleaire, hopital Tenon, 75 - Paris (France); Perie, S.; Lacau St-Guily, J. [Universite Pierre-et-Marie-Curie, ORL, hopital Tenon, 75 - Paris (France); Nataf, V. [Universite Pierre-et-Marie-Curie, hopital Tenon, Service de radiopharmacie, 75 - Paris (France); Balogova, S. [Faculte de medecine, universite Comenius, Bratislava (Slovakia)

    2010-07-15

    The utility of preoperative scintigraphy in case of secondary hyperparathyroidism is questioned by some authors. Obviously, an imaging modality that will detect all hyperplastic glands, including the ectopic ones, would be of interest in those patients at high risk for surgery. However, scintigraphy has a limited detection rate in some patients. We investigated whether one of the following parameters would identify a subgroup of patients in whom the detection rate would be optimal: age, gender, hemodialysis and duration since its onset, and plasma levels of parathyrin (P.T.H.). Methods: Retrospective series of 38 patients referred for preoperative parathyroid scintigraphy due to secondary hyperparathyroidism who then underwent para thyroidectomy. Scintigraphy was performed 20 min and then 3 h after injection of 8 MBq/kg of sestamibi ({sup 99m}Tc) with a previous ingestion of 0.1 MBq/kg iodine-123, 3 h before. Result: No significant correlation was observed between the number of glands detected on scintigraphy (and confirmed by postoperative histology) and plasma P.T.H. levels (r = -0.17). A weak positive correlation (r = +0.34) was noted in the group of six non-hemo dialysed patients. No significant relationship between this number of detected glands and a clinical parameter was observed. Conclusion: In our experience, these parameters do not permit to select, among patients with secondary hyperparathyroidism and scheduled for para thyroidectomy, those who will better benefit from parathyroid scintigraphy. (authors)

  12. Preparation and assessment of [{sup 99m}Tc]technetium aquacarbonyl complexes with 1,2-diaminoethane-N-substituted ligands for tumor detection; Preparo e avaliacao de complexos de [{sup 99m}Tc]tecnecio aquacarbonil com ligantes 1,2-diaminoetano-N-substituidos para deteccao de tumores

    Energy Technology Data Exchange (ETDEWEB)

    Radin, Adriano

    2010-07-01

    Over least 15 years the complex [[{sup 99m}Tc](H{sub 2}0){sub 3}(CO){sub 3}]{sup +} has been used as an intermediary to obtain technetium radiopharmaceuticals for applications in cardiology, neurology and oncology. Two important characteristics of this molecule are: the facility for obtaining that compound from aqueous solutions and the easiness of substituting H{sub 2}O molecules by atoms of other ligand molecules. In this project we prepared new complexes [[{sup 99m}Tc](CMN{sup S001-3})(H{sub 2}O)(CO){sub 3}]{sup +}, where (CMNS001) = N-[(4-methoxy) benzyl]-1,2-diaminoethane, (CMNS003) = N,N'-bis-[(4-methoxy)benzyl]-1,2-diaminoethane, and assessed the uptake of these complexes in murine melanoma cancer cell B16F10 and breast cells MCF-7 and MDA-MD-231, and compared with [[{sup 99m}](MIBI){sub 6}]{sup +} uptake. In vitro uptake for both new technetium complex reached values close to 5%, for all cell lines, whereas the [[{sup 99m}Tc](MIBI){sub 6}]{sup +} uptake was close to 1 %. The assessment of subcellular distribution showed high accumulation of the new complex in the membrane fraction, for MDAMB-231, while for B16F10 accumulation occurred both in membrane and cytoplasm; the concentration of [[{sup 99m}Tc](MIBI){sub 6}]{sup +} was mainly in the cytoplasm portion. Biodistribution study in mice allowed to observe the capture of up to 1.6% of the administered dose per gram of tumor tissue for the complex [[{sup 99m}Tc](CMNS001)(H{sub 2}O)(CO){sub 3}]{sup +}, whereas other organs such as heart, lung and muscle, showed uptake of about 5.6%, 6.4% and 2%, respectively. The complexes in this work showed a high rate of uptake in vitro, but was not reproduced in vivo model, which can be related to low concentration of the complexes inside the cells and reduced vascularity of tumor tissue, with lower intake of complex through the blood system. (author)

  13. Study of the optical densitometry measurement for analysis of osseous and renal scintigraphies with MDP-{sup 99m} Tc and DMSA-{sup 99m} Tc; Estudo da medida densitometrica optica para analise de cintilografias osseas e renais obtidas com MDP-{sup 99m} Tc e DMSA-{sup 99m} Tc

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Maria Clara Carvalho da

    2001-07-01

    The optical densitometry can be used as a technique for radiographic images measurement to determine the radiopharmaceutical uptake or reception by human organs. In this paper were studied optical densitometry measurements using about 600 osseous scintigraphies produced with MDP-{sup 99m}Tc in eighteen parts of the human skeleton from 170 healthy and sick people and also in 56 renal scintigraphies produce with DMSA-{sup 99m}Tc in the same number of people both healthy and sick that performed this second examination type. The results showed that for the skeleton, the optical densitometry can give a correct analysis of the person pathological areas and, if it will be automated, through microcomputer it can become a useful instrument to perform diagnoses with smaller probability of mistakes than the visual traditional method performed by the physician, turning the most effective individual treatment. For the kidney, the optical densitometry can visualize the relative renal function showing the uptake percentage of the radiopharmaceutical by the organ, but it is not capable to supply a complete diagnosis. (author)

  14. A Pilot Comparison of 18F-fluorocholine PET/CT, Ultrasonography and 123I/99mTc-sestaMIBI Dual-Phase Dual-Isotope Scintigraphy in the Preoperative Localization of Hyperfunctioning Parathyroid Glands in Primary or Secondary Hyperparathyroidism: Influence of Thyroid Anomalies.

    Science.gov (United States)

    Michaud, Laure; Balogova, Sona; Burgess, Alice; Ohnona, Jessica; Huchet, Virginie; Kerrou, Khaldoun; Lefèvre, Marine; Tassart, Marc; Montravers, Françoise; Périé, Sophie; Talbot, Jean-Noël

    2015-10-01

    We compared (18)F-fluorocholine hybrid positron emission tomography/X-ray computed tomography (FCH-PET/CT) with ultrasonography (US) and scintigraphy in patients with hyperparathyroidism and discordant, or equivocal results of US and (123)I/(99m)Tc-sesta-methoxyisobutylisonitrile (sestaMIBI) dual-phase parathyroid scintigraphy. FCH-PET/CT was performed in 17 patients with primary (n = 11) lithium induced (n = 1) or secondary hyperparathyroidism (1 dialyzed, 4 renal-transplanted).The reference standard was based on results of surgical exploration and histopathological examination. The results of imaging modalities were evaluated, on site and by masked reading, on per-patient and per-lesion bases.In a first approach, equivocal images/foci were considered as negative. On a per-patient level, the sensitivity was for US 38%, for scintigraphy 69% by open and 94% by masked reading, and for FCH-PET/CT 88% by open and 94% by masked reading. On a per-lesion level, sensitivity was for US 42%, for scintigraphy 58% by open and 83% by masked reading, and for FCH-PET/CT 88% by open and 96% by masked reading. One ectopic adenoma was missed by the 3 imaging modalities. Considering equivocal images/foci as positive increased the accuracy of the open reading of scintigraphy or of FCH-PET/CT, but not of US. FCH-PET/CT was significantly superior to US in all approaches, whereas it was more sensitive than scintigraphy only for open reading considering equivocal images/foci as negative (P = 0.04). FCH uptake was more intense in adenomas than in hyperplastic parathyroid glands. Thyroid lesions were suspected in 9 patients. They may induce false-positive results as in one case of oncocytic thyroid adenoma, or false-negative results as in one case of intrathyroidal parathyroid adenoma. Thyroid cancer (4 cases) can be visualized with FCH as with (99m)Tc-sestaMIBI, but the intensity of uptake was moderate, similar to that of parathyroid hyperplasia.This pilot study confirmed that

  15. Contribution of {sup 99m}Tc-sestamibi scintigraphy by double phase in the exploration of hyperparathyroidism. Report of 20 cases; Apport de la scintigraphie parathyroidienne au {sup 99m}Tc-MIBI en double phase dans l'exploration des hyperparathyroidies. A propos de 20 cas

    Energy Technology Data Exchange (ETDEWEB)

    Ghfir, I.; Ben Rais, N. [Hopital Ibn Sina, CHU de Rabat, Service de Medecine Nucleaire de Rabat (Morocco)

    2008-11-15

    Introduction {sup 99m}Tc-sestamibi parathyroid scintigraphy is a means of functional imaging allowing the exploration of hyperparathyroidism. The aim of our study is to demonstrate the utility of double-phase {sup 99m}Tc-sestamibi scintigraphy in the exploration of the secreting abnormal parathyroid gland. Materials and methods We report, through this work, the observation of 20 patients followed for a biologically ascertained hyperparathyroidism and explored, for the majority of them, by ultrasonography and/or computed tomography. All our patients benefited from a double-phase {sup 99m}Tc-sestamibi scintigraphy. Results On the 20 studied cases, the sex-ratio was equal to 1, two patients exhibited three high uptake foci at the {sup 99m}Tc-sestamibi scintigraphy, six exhibited two foci, twelve exhibited one parathyroid focus. In our series, 80% of patients exhibited secondary hyperparathyroidism and 20% exhibited a primary hyperparathyroidism. The pathologic exam revealed four cases of parathyroid adenoma and 16 parathyroid cases of hyperplasia. Discussion The double-phase {sup 99m}Tc-sestamibi scintigraphy contributes to the orientation and the improvement of the surgical attitude of the hyperparathyroidism, insofar as it could affirm the multiplicity of some adenomas, the diffuse form of some hyperplasia, and especially ectopic localization of the abnormal parathyroid gland.

  16. The Relationship Between Technetium-99m-Methoxyisobutyl Isonitrile Parathyroid Scintigraphy and Hormonal and Biochemical Markers in Suspicion of Primary Hyperparathyroidism

    OpenAIRE

    Güler Silov; Ayşegül Özdal; Zeynep Erdoğan; Özgül Turhal; Hatice Karaman

    2013-01-01

    Objective: Technetium-99m-methoxyisobutyl isonitrile (Tc-99m MIBI) has been widely used to evaluate hyperfunctioning autonomous parathyroid glands in patients with elevated intact parathyroid hormone (iPTH) and/or calcium (Ca) level. The aim of this study was to evaluate the relationship between Tc-99m MIBI parathyroid scintigraphy and hormonal and biochemical markers in suspicion of primary hyperparathyroidism (PHPT). Material and Methods: Dual-phase Tc-99m MIBI parathyroid scintigraphy and ...

  17. Development of methods of labeling pentavalent DMSA with {sup 99m}Tc and {sup 188}Re; Desenvolvimento de metodos para marcacao de DMSA pentavalente com {sup 99m}Tc e {sup 188}Re

    Energy Technology Data Exchange (ETDEWEB)

    Brambilla, Tania de Paula, email: jtoniolo@ipen.br

    2009-07-01

    Technetium-99 m is the most useful radionuclide in diagnostic imaging procedures in Nuclear Medicine, more than 80 percent of radiopharmaceuticals are {sup 99m}Tc-labeled compounds. {sup 99m}Tc-DMSA(V) has been used for imaging of soft tissue, head and neck tumors. It shows a particularly high specificity for medullary thyroid carcinoma and bone metastases in a variety of cancers. Biodistribution studies of {sup 188}Re-DMSA(V) have shown that its general pharmacokinetic properties are similar to that of {sup 99m}Tc-DMSA(V), so this agent could be used for targeted radiotherapy of these tumors. The aim of this work is the development of methods of labeling DMSA(V) with {sup 99m}Tc and {sup 188}Re. {sup 99m}Tc-DMSA(V) can be prepared by two methods. One of them is the indirect one, through the use of a commercial kit of DMSA (III), by adjusting the pH from 2.5 to {approx} 8.5 with NaHCO{sub 3}. This method was evaluated and optimized presenting high labeling yields. The other method is the direct one, through the preparation of a lyophilised kit ready for labeling with {sup 99m}Tc, being the method of interest of this work, due to the easy of its clinical use. The most adequate formulation of the kit was: 1.71 mg of DMSA, 0.53 mg of SnCl{sub 2}.2H{sub 2}O and 0.83 mg of ascorbic acid (pH 9). Labeling yields higher than 95% were achieved labeling this kit with 1 to 2 m L of {sup 99m}Tc with activities up to 4736 MBq (128 mCi). The kit was stable up to 6 months and biodistribution studies confirmed the quality of the DMSA (V) labeled with {sup 99m}Tc using this kit. The reduction potential of Re is lower than the one for Tc, so the labeling conditions of {sup 188}Re-DMSA(V) are different from the ones used for {sup 99m}Tc- DMSA(V). {sup 188}Re-DMSA(V) is prepared in acid solution, that makes it possible to use the DMSA (III) commercial kit developed for labeling with {sup 99m}Tc, prepared in pH 2.5, for labeling with {sup 188}Re. Labeling yields higher than 95% were

  18. Clinical significance of exercise-induced ST segment depression in patients with lateral myocardial infarction involving the left circumflex artery. Evaluation by exercise 99mTc-MIBI myocardial scintigraphy

    International Nuclear Information System (INIS)

    The aim of this study is to clarify the causes of exercise-induced ST-segment depression in patients with broad lateral old myocardial infraction involving left circumflex branch (LCX) (LCX-OMI) without ischemia on exercise scintigraphy. Twenty one patients (M/F=11/10, age=62±19 years) with myocardial infraction involving LCX (LCX-MI), but without fill-in on exercise and rest MIBI quantitative gated SPECT (QGS), were selected. They were divided into two groups of Group ST(+) (n=11, with significant ST depression (max-2.8±0.4 mm), Group ST (-) (n=10) without ST depression. On 20 SPECT segments of both exercise and rest SPECT, we scored uptake score as defect score (DS) (0=normal to 3=defect) and wall motion as wall motion score (WMS) (0=normal to -5=dyskinesis) and summed DS (TDS) and WMS (TWMS) in LCX region, furthermore, calculated the difference of TWMS (ΔTWMS {exercise-rest}), end diastolic volume (EDV) and ejection fraction (EF) during exercise were compared between the two groups. Group ST(+) showed significantly (p2-4 without ischemia in LCX-MI was observed in patients with broad LCX-MI, low EF, and was related to impaired wall motion in LCX region. ST depression in V2-4 was considered to appear as miller image of ST elevation at postero-inferior wall due to disturbed wall motion on exercise. (author)

  19. Is Brown Adipose Tissue Visualization Reliable on 99mTc-Methoxyisobutylisonitrile Diagnostic SPECT Scintigraphy?

    Science.gov (United States)

    Haghighatafshar, Mahdi; Farhoudi, Farinaz

    2016-01-01

    The 99mTc-MIBI has been used with great value as a diagnostic technique in patients with primary hyperparathyroidism. False-positive scans may occur due to misinterpretation of the physiologic distribution of the 99mTc-MIBI. Reviewing consecutive SPECT scans, we evaluated this possibility and assessed how frequently brown adipose tissue (BAT) is seen on 99mTc-MIBI scintigraphy. Here, we retrospectively reviewed scans of consecutive patients who were evaluated for parathyroid adenomas from March 2015 to June 2015, using dual-phase (early and delayed) planar imaging and SPECT. We identified 60 patients (48 female and 12 male; mean age, 52.25 ± 15.20 years; range, 22-86 years).We detected the presence of 99mTc-MIBI uptake in BAT in 20 of 60 patients (33.33%) in the neck. Although the patients with T99mc-MIBI uptake in BAT were younger (mean age, 48.85 ± 15.27 years, range, 26-73 years) than the patients with no 99mTc-MIBI uptake (mean age, 53.95 ± 15.07 years, range, 22-86 years), this difference was not statistically significant (P = 0.224). The percentage of female patients with BAT detection was higher (17/48 patients; 37.5%) than that of the male population (3/12 patients; 25%), this difference was not also statistically significant (P = 0.85).In patient population referred to 99mTc MIBI scintigraphy of the parathyroid glands, uptake of 99mTc-MIBI in BAT should not be misinterpreted with 99mTc-MIBI-avid-tumors. Fused SPECT/CT images (not SPECT-only) are necessary to distinguish BAT from bone, muscle, thyroid, myocardium, parathyroids, and other structures in the neck and chest. PMID:26765463

  20. Standardization of a method to calculate absolute renal uptake of {sup 99m} Tc-DMSA in children; Padronizacao do metodo para calculo da captacao renal absoluta do {sup 99m}Tc-DMSA em criancas

    Energy Technology Data Exchange (ETDEWEB)

    Ono, Carla Rachel; Sapienza, Marcelo Tatit; Watanabe, Tomoco; Costa, Paulo Luiz Aguirre; Okamoto, Miriam Roseli Yoshie; Garcez, Alexandre Teles; Buchpiguel, Carlos Alberto [Sao Paulo Univ., SP (Brazil). Hospital das Clinicas. Servico de Medicina Nuclear]. E-mail: crachelo@usp.br; Machado, Beatriz Marcondes; Machado, Marcia Melo Campos [Paulo Univ., SP (Brazil). Faculdade de Medicina. Hospital Universitario; Liberato Junior, Waldyr de Paula

    2006-01-15

    Objective:To standardize a method and determine normal values for absolute renal uptake of 99m Tc-DMSA in children with normal creatinine clearance. Materials and methods: Twenty-two children (between 7 months and 10 years of age; mean 4.5 years) without clinical evidence of renal disease were studied using 99m Tc-DMSA scintigraphy. Eighteen had normal renal ultrasonography, micturating urethrocystography, creatinine clearance and visual interpretation of the scintigraphy with 99m Tc-DMSA. Four children were excluded, one with incomplete creatinine clearance and three due to reduction in the creatinine clearance. Absolute renal uptake of 99m Tc-DMSA (DMSA-Abs) was expressed as the fraction of the administered dose retained by each kidney six hours after administration of the radiopharmaceutical. Results: DMSA-Abs was 21.8 +- 3.2% for the right kidney and 23.1 +-3.3% for the left kidney. There was no correlation between renal uptake and the age groups studied, although there was a tendency to an increase in the creatinine clearance with age. Conclusion: Normal values of DMSA-Abs can be used as an additional parameter for the initial diagnostic evaluation and during follow-up of renal diseases, mainly when bilateral impairment of renal function is suspected or in a patient with a single functioning kidney (in which renal differential function is of limited value). (author)

  1. {sup 99m} Tc-UBI 29-41: radiolabelled antimicrobial peptide for the diagnostic by image of infectious processes; {sup 99m} Tc-UBI 29-41: peptido antimicrobiano radiomarcado para el diagnostico por imagen de procesos infecciosos

    Energy Technology Data Exchange (ETDEWEB)

    Ferro F, G.; Ramirez C, F.M. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico); Murphy, C.A. de; Pedraza L, M.; Rodriguez C, J. [INCMNSZ, 14000 Mexico D.F. (Mexico); Melendez A, L. [Facultad de Medicina, UAEM, 50000 Toluca, Estado de Mexico (Mexico)

    2005-07-01

    Recently the radiolabelled anti microbic peptides has intended as new radiopharmaceuticals for distinguish a bacterial infection of a sterile inflammatory process through a diagnostic image. The ubiquicidine 29-41 (UBI), it is a fragment of an anti microbic cationic peptide located in the human skin whose sequence of amino acids is Thr-Gly-Arg-Ala-Lys-Arg-Arg-Met-Gln-Tyr-Asn-Arg-Arg. The objective of this study was to develop a derived of the UBI 29-41 radiolabelled with Tc-99m in high radiochemical purity and to evaluate the feasibility of using it in the specific detection of infectious focuses. The molecular structures of the UBI as well as of other 2 cationic peptides used as control (Tat-1-Scr and Tat-2-Scr), they were calculated and optimized in their more stable configuration by molecular mechanics procedures and quantum mechanics. Once established the site for the labelled with {sup 99m} Tc, the three complexes were represented by the general formula [Tc(V)(O)(H{sub 2}O){sub 2} (Lys{sub n=1,2}-Arg{sub n=0.1}-peptide)]{sup 10+,11+}, with potential energy of 104.5 Kcal/mol, 95.6 Kcal/mol and 90.8 Kcal/mol for the {sup 99m}Tc-Tat-1-Scr, {sup 99m} Tc-Tat-2-Scr and {sup 99m}Tc-UBI 29-41 respectively. The three radio complexes got ready experimentally and their stability in vitro saline solution is evaluated, human serum and cysteine solutions. The experimental results correlated appropriately with the calculated data. The specificity in vitro was carried out evaluating the percentage of union of the {sup 99m}Tc-UBI 29-41 as well as of the control peptides at bacteria of S. aureus and two tumoral cellular lines (LS174T and ACHN). The In vivo specificity was determined using Balb-C mice with induction in a paw of an infectious process and in another paw of a sterile inflammatory process. Simultaneously it was administered to the mice with infection and inflammation induced {sup 99m}Tc-UBI 29-41 and {sup 67}Ga-citrate this last an unspecific radiopharmaceutical

  2. Development of lyophilized kit of Tin-Glucoheptonate for in vitro labeling of leucocytes with {sup 99m}Tc; Desenvolvimento de reagente liofilizado de glucoheptonato-estanho para marcacao de leucocitos com Tecnecio-99m in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Nascimento, Rosemeire Fagundes

    2007-07-01

    The study and localization of inflammatory and infection process in Nuclear Medicine represents a relevant tool in diagnostic procedures. In same cases, the diagnostic is easy and based on anamnesis and clinical observation; in other cases, the patients are asymptomatic or present non specific symptoms that difficult the diagnostic. The early diagnostic of inflammatory or infectious process allow the early introduction of therapy and prevents complications. Farther, the differentiation between inflammation and infection is of extreme importance as well as the localization of the focus. The use of labeled leucocytes, studied and applied in much pathologies, is the method of choice for the visualization of inflammation and infection. The scintigraphy using labeled leucocytes was introduced at 1976 by McAffe and Thakur and since of this is used in the diagnostic of different pathologies related to leucocyte infiltration like intestinal inflammatory disease, bone or prosthetic-vascular infections. The in vitro labeling of leucocytes with {sup 111}In was performed using oxime or tropolone as ligand and with {sup 99m}Tc using hexamethylpropylene amine oxime (HMPAO) as ligand, resulting in a lipophilic complex. The {sup 99m}Tc-HMPAG complex was preferably employed in many indications and countries do to the ideal physical properties of {sup 99m}Tc that results in low dose to the patient. However, the labeling employing the HMPAO complex results in some disadvantages like the low stability of the complex, and some requirements related to the {sup 99m}Tc elution (like the time pos elution), beyond the high cost of the compound that is imported. The aim of this work was the development of a tin-glucoheptonate lyophilized kit for in vitro leucocytes labeling with {sup 99m}Tc using the pre-stannization method. The optimization of the labeling technique was developed using leucocytes isolated from total blood and employing different volumes of the tinglucoheptonate reagent and

  3. Staging and therapy monitoring of multiple Myeloma by 99mTc-Sestamibi Scintigraphy: a five year single center experience

    International Nuclear Information System (INIS)

    The aim of the present study was the evaluation of the diagnostic value of 99mTc-sestamibi (MIBI) in the detection of bone marrow involvement in patients suffering from multiple myeloma (MM) and its possible role in the follow-up. Between 1998 and 2003, 68 patients with MM and 42 pts with monoclonal gammopathy of undetermined significance (MGUS) were consecutively enrolled in this study. 51/68 MM patients had active disease (AD), 11/62 were in complete remission (CR) and 6/68 in partial remission (PR) after chemotherapy. 18 patients with MM repeated a 99mTc-MIBI scintigraphic study at least 2 months after high-dose chemotherapy. All the scans were score semi quantitatively according to extension and intensity of tracer uptake. All MGUS pts had a negative 99mTc-MIBI. As far as the MM pts are concerned, 54/68 (49%) pts (48 with AD, 5 with PR and 1 with CR) had a positive 99mTc-MIBI scan, while the 99mTc-MIBI scan was negative in 14/68 pts (10 with CR, 1 with PR and 3 with AD). The overall sensitivity of the 99mTc-MIBI scintigraphy was 92%; specificity was 96%. In the follow up of the pts treated with chemotherapy 99mTc-MIBI closely paralleled the activity of myeloma bone disease. In conclusion, these results indicate that 99mTc-MIBI scintigraphy closely reflects myeloma disease activity in the bone marrow, and that a negative 99mTc-MIBI scan in patients with suspected MM clearly, though not absolutely, indicates absence of disease or clinical remission. The results of this study suggest a clear diagnostic value of 99mTc-MIBI scintigraphy in patients with MM and its potential role during the follow-up for the monitoring of MM bone disease

  4. Development of a formulation lyophilized for the obtention of a antimicrobial peptide Ubiquicidine labelled with {sup 99m} Tc; Desarrollo de una formulacion liofilizada para la obtencion del peptido antimicrobiano Ubiquicidina marcado con Tc-99m

    Energy Technology Data Exchange (ETDEWEB)

    Palomares R, P.; Hernandez B, C.A.; Contreras N, G.; Garcia P, M.L.; Pantoja H, I.E. [UAEM, Toluca (Mexico); Ferro F, G. [ININ, A.P. 18-1027, 11801 Mexico D.F. (Mexico)]. E-mail: patodish@hotmail.com

    2004-07-01

    The {sup 99m} Tc-UBI 29-41 are a labelled fragment of the antimicrobial human peptide Ubiquicidine proposed as a new radiopharmaceutical able to differentiate an infectious process of an inflammatory one through the gamma graphic image. It has been demonstrated that the {sup 99m} Tc-UBI 29-41 unite to bacteria in vitro and that accumulates in infection sites in human with minimum captivation in inflammation sites. In this work the development of a pharmaceutical lyophilized formulation is presented for the instantaneous marked one of the UBI 29-41 with {sup 99m} Tc. The selection of the components of the formulation settled down by means of the employment of an experimental design of 3 factors with mixed levels, evaluating the effect of the diluent type, concentration of tinny chloride and the reaction volume. The obtained formulations showed to be stable until for 6 months, being obtained complexes of the radiolabelled peptide with radiochemical purity > 95 % in sterile form and apirogen. The developed pharmaceutical form, will facilitate the routinary use of this new radiopharmaceutical in the diverse hospital departments of nuclear medicine. (Author)

  5. Development of methodology for evaluation of {sup 99m}Tc and {sup 131}I incorporated activities during lactation; Desenvolvimento de metodologia para avaliacao da atividade de {sup 99m}Tc e {sup 131}I em lactantes

    Energy Technology Data Exchange (ETDEWEB)

    Santos, L. [Universidade Federal do Rio de Janeiro (IF/UFRJ), RJ (Brazil). Inst. de Fisica; Dantas, A.L.A.; Mesquita, S.A. [Universidade Federal do Rio de Janeiro (IF/UFRJ), RJ (Brazil). Inst. de Fisica; Oliveira, S.M.V., E-mail: adantas@ird.gov.br, E-mail: silvia@ird.gov.br [Universidade Federal do Rio de Janeiro (IF/UFRJ), RJ (Brazil). Inst. de Fisica; Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil). Div. de Dosimetria

    2012-07-01

    Internal contamination of babies may occur for milk ingestion or inhalation of mothers occupationally exposed to ionizing radiation with possible incorporation or mothers submitted to medical exposures during lactation. Radionuclide concentrations in the mother's milk may cause organ absorbed doses in the babies proportionally to the breast volumes. Milk analysis allow to determine activities ingested by the babies by determining the peak of mother's milk considering the decrease of the activity rate and the milk activities drunk at different time intervals. The work had the aim to develop simulators and methodology to evaluate {sup 99m}Tc and {sup 131}I in lactation, in the following steps: to prepare standard solution of contaminated milk separately with {sup 99m}Tc and {sup 131}I; to build four breast simulators (600 g and 800 g) and respective calibration for two geometries (breast and whole-body) in the Whole-Body Counter Unit in Instituto de Radioprotecao e Dosimetria. The results demonstrated the system efficiency to determine {sup 99m}Tc and {sup 131}I activities in breasts during the lactation period. The methodology for positioning in the 'breast geometry' seemed to be more efficient than the 'whole-body geometry' for different breast volumes. The experiment allows achieving better evaluation of internal dosimetry of mothers and their young children. (author)

  6. Effect of expression of P-glycoprotein on technetium-99m methoxyisobutylisonitrile single photon emission computed tomography of brain tumors

    Energy Technology Data Exchange (ETDEWEB)

    Shibata, Yasushi; Matsumura, Akira; Nose, Tadao [Tsukuba Univ., Ibaraki (Japan). Inst. of Clinical Medicine

    2002-08-01

    The expression of P-glycoprotein was investigated imunohistochemically in 26 brain tumor tissues and compared with the findings of technetium-99m methoxyisobutylisonitrile single photon emission computed tomography ({sup 99m}Tc-MIBI SPECT) to clarify the effect of P-glycoprotein on the diagnostic accuracy. P-glycoprotein labeling index of both tumor cells and vascular endothelial cells showed no clear relationship with the findings of {sup 99m}Tc-MIBI SPECT imaging. Expression of P-glycoprotein has no effect on the diagnostic accuracy of {sup 99m}Tc-MIBI SPECT. (author)

  7. Radiation exposure of the patient in diagnostic nuclear medicine. Experimental studies of the biokinetics of {sup 111}In-DTPA-D-Phe{sup 1}-octreotide, {sup 99m}Tc-MIBI, {sup 14}C-triolein and {sup 14}C-urea, and development of dosimetric models

    Energy Technology Data Exchange (ETDEWEB)

    Leide Svegborn, S

    1999-03-01

    Biokinetic and dosimetric models for a number of clinically used radiopharmaceuticals, for which information on the radiation dosimetry is scarce, have been produced. On patients undergoing investigations with {sup 111}In-DTPA-D-Phe{sup 1}-octreotide (for diagnosis of neuroendocrine tumours) and {sup 99m}Tc-MIBI (for myocardial perfusion imaging), whole body gamma camera scanning was performed several times after administration of the radiopharmaceutical. Total body and organ activity content was determined using the geometric mean of the number of counts in two 180 deg opposed planar images. A thorough investigation of sources influencing the accuracy of the quantification of activity was carried out, showing an overall uncertainty varying from 10% to 30% for organs with a significant uptake and 5% for the whole body. The activity in blood and urine was also measured. {sup 111}In-DTPA-D-Phe{sup 1}-octreotide was predominantly excreted via the kidney-bladder system and a typical investigation with 1200 MBq resulted in an effective dose of 8.4 mSv (0.076 mSv/MBq). {sup 99m}Tc-MIBI was to a great extent excreted via the gastrointestinal tract and an investigation with 1200 MBq resulted in an effective dose of 13 mSv (0.011 mSv/MBq). Accelerator mass spectrometry (AMS) was used to investigate the possibility to measure ultra-low activity concentrations of {sup 14}CO{sub 2}, in exhaled air from patients undergoing {sup 14}C-breath tests, with special application to {sup 14}C-triolein (for study of fat malabsorption). AMS was proven to be a useful technique for long-term retention studies of {sup 14}C, and was used together with liquid scintillation counting in an investigation of the biokinetics of {sup 14}C-urea in adult and paediatric patients (for diagnosis of Helicobacter pylori infection in the upper gastrointestinal tract). The effective dose for {sup 14}C-urea was 0.019 mSv/MBq for adults and from 0.041 to 0.019 mSv/MBq for seven- to fourteen -year-old children

  8. Radiation exposure of the patient in diagnostic nuclear medicine. Experimental studies of the biokinetics of 111In-DTPA-D-Phe1-octreotide, 99mTc-MIBI, 14C-triolein and 14C-urea, and development of dosimetric models

    International Nuclear Information System (INIS)

    Biokinetic and dosimetric models for a number of clinically used radiopharmaceuticals, for which information on the radiation dosimetry is scarce, have been produced. On patients undergoing investigations with 111In-DTPA-D-Phe1-octreotide (for diagnosis of neuroendocrine tumours) and 99mTc-MIBI (for myocardial perfusion imaging), whole body gamma camera scanning was performed several times after administration of the radiopharmaceutical. Total body and organ activity content was determined using the geometric mean of the number of counts in two 180 deg opposed planar images. A thorough investigation of sources influencing the accuracy of the quantification of activity was carried out, showing an overall uncertainty varying from 10% to 30% for organs with a significant uptake and 5% for the whole body. The activity in blood and urine was also measured. 111In-DTPA-D-Phe1-octreotide was predominantly excreted via the kidney-bladder system and a typical investigation with 1200 MBq resulted in an effective dose of 8.4 mSv (0.076 mSv/MBq). 99mTc-MIBI was to a great extent excreted via the gastrointestinal tract and an investigation with 1200 MBq resulted in an effective dose of 13 mSv (0.011 mSv/MBq). Accelerator mass spectrometry (AMS) was used to investigate the possibility to measure ultra-low activity concentrations of 14CO2, in exhaled air from patients undergoing 14C-breath tests, with special application to 14C-triolein (for study of fat malabsorption). AMS was proven to be a useful technique for long-term retention studies of 14C, and was used together with liquid scintillation counting in an investigation of the biokinetics of 14C-urea in adult and paediatric patients (for diagnosis of Helicobacter pylori infection in the upper gastrointestinal tract). The effective dose for 14C-urea was 0.019 mSv/MBq for adults and from 0.041 to 0.019 mSv/MBq for seven- to fourteen -year-old children, resulting in an effective dose of approximately 0.002 mSv per investigation

  9. Prediction of sensitivity to anticancer agents for patients with advanced or recurrent breast cancer by Tc-99m sestamibi

    International Nuclear Information System (INIS)

    Tc-99m Sestamibi (99mTc-MIBI) is known to be a substrate of P-glycoprotein (P-gp) that effluxes the drugs out of cancer cells. The overexpression of P-gp involved in multidrug resistance phenomenon in patients with advanced or recurrent breast cancers was shown in the plasma membrane of breast cancer cells. In this study, we examined the usefulness of 99mTc-MIBI scintigraphy for the prediction of sensitivity to anticancer agents in 8 cases with advanced or recurrent breast cancer. The retrospective analysis showed that the sensitivity to the chemotherapy could be evaluated in 3 cases by 99mTc-MIBI scintigraphy, but in the other 5 cases 99mTc-MIBI scintigraphy was not eligible for the prediction of sensitivity. Two out of 3 cases showed over 50% in reduction rate of target tumors (PR) with higher accumulation of 99mTc-MIBI, while another case with PD showed lower. These results suggest that the accumulation of 99mTc-MIBI could be associated with the sensitivity to P-gp-related anticancer agents, and that the functional analysis of P-gp by 99mTc-MIBI might be useful for the prediction of responsiveness of chemotherapy in patients with breast cancer. (author)

  10. Evaluation of aptamers labelled with {sup 99m}Tc for identification of Staphylococcus aureus bacteria; Avaliacao de aptameros marcados com {sup 99m}Tc para identificacao de focos infecciosos de Staphylococcus aureus

    Energy Technology Data Exchange (ETDEWEB)

    dos Santos, Sara Roberta

    2014-06-01

    Staphylococcus aureus is specie of great medical importance because it is often associated with many infections in humans. This bacterium can cause diseases ranging from simple infections to life-threatening infections such as endocarditis, pneumonia, meningitis, toxic shock syndrome, septicemia, osteomyelitis, among others. S. aureus is the most commonly agent found in infections of the skin and soft tissues, bone infections and bone prostheses. The difficulty in early detection of specific foci caused by bacteria has raised the need to search for new techniques for this purpose. Diagnosis by scintigraphy has advantages over other methods because it is able to identify damage tissues without the need of invasive procedures and is able to perform an early diagnosis even before anatomic changes. Thus, nuclear medicine could contribute to an accurate diagnosis of bacterial infections, since specific radiopharmaceuticals were developed. Aptamers are oligonucleotides that have high affinity and specificity for their molecular targets and are emerging as a new class of molecules for radiopharmaceuticals development. Radiolabeled aptamers specific to the infectious agents, could give a significant contribution to the infection diagnosis by scintigraphy. In this study, aptamers selected to S. aureus were labeled with {sup 99m}Tc and used for the bacteria identification in vitro and in vivo. The aptamers labeled with {sup 32}P and incubated in vitro with S. aureus cells showed high affinity for the bacterial cells when compared with the library of oligonucleotides with random sequences used as control. The aptamers labeled with {sup 99m}Tc also showed affinity for S. aureus cells when compared with the library, but unspecific binding was also verified. The {sup 99m}Tc labelled aptamers were stable in 0.9% saline, plasma of Swiss mice and in excess of cysteine. The in vivo biodistribution studies using Swiss mice with intramuscular infection in the right thigh showed that

  11. Intraoperative injection of technetium-{sup 99m}-dextran 500 for the identification of sentinel lymph node in breast cancer; Injecao intraoperatiria de dextran-500-{sup 99m}-tecnecio para identificacao do linfonodo sentinela em cancer de mama

    Energy Technology Data Exchange (ETDEWEB)

    Delazeri, Gerson Jacob, E-mail: gersonjacob@gmail.co [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Programa de Pos-Graduacao em Medicina e Ciencias Medicas; Xavier, Nilton Leite [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Fac. de Medicina. Dept. de Ginecologia e Obstetricia; Menke, Carlos Henrique; Bittelbrunn, Ana Cristina [Hospital de Clinicas (HCPA), Porto Alegre, RS (Brazil). Servico de Mastologia; Spiro, Bernardo Leao [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Fac. de Medicina. Dept. de Radiologia; Mosmann, Marcos Pretto [Hospital de Clinicas (HCPA), Porto Alegre, RS (Brazil). Servico de Medicina Nuclear; Graudenz, Marcia Silveira [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS (Brazil). Dept. de Patologia

    2010-07-01

    Purpose: to determine the efficacy of intraoperative injection of Dextran-500-{sup 99m}-technetium (Tc) for the identification of the sentinel lymph node (SLN) in breast cancer and analyze time to label the SLN in the axillary region. Methods: a prospective study between April 2008 and June 2009, which included 74 sentinel lymph node biopsies (SLNB) in patients with breast cancer in stages T1N0 and T2N0. After induction of anesthesia, 0.5 to 1.5 mCi of Dextran-500-{sup 99m}-Tc filtered 0.22 {mu}m in a volume of 5 mL was injected intraoperative using the subareolar technique for SLNB. After labeling with the radioisotope, 2 mL of patent blue was injected. The time elapsed between injection and the axillary hot spot, the in vivo and ex vivo counts of the hottest nodes, the background count, and the number of SLN identified were documented. Data were analyzed using descriptive statistics with SPSS program, version 18. Results: we identified the SLN in 100% of cases. The rate of SLN identification with the probe was 98% (73/74 cases). In one case (1.35%) the SLN was labeled only with the blue dye. The mean dose of radioisotope injected was 0.97{+-}0.22 mCi. The average time to label the SLN was 10.7 minutes ({+-}5.7 min). We identified on average of 1.66 SLN labeled with the radioisotope. Conclusion: the procedure for SLN identification with an intraoperative injection of the radioisotope is oncologically safe and comfortable for the patient, providing agility to the surgical team. (author)

  12. Development of nano radiopharmaceutical based on Bevacizumab labelled with Technetium-99m for early diagnosis of gastrointestinal stromal tumor; Desenvolvimento de nanorradiofarmaco a base de Bevacizumabe marcado com tecnecio-99m para diagnostico precoce do tumor estromal gastrointestinal

    Energy Technology Data Exchange (ETDEWEB)

    Braga, Thais Ligiero

    2015-06-01

    The development of new radiopharmaceuticals is an essential activity to improve nuclear medicine, and essential for the early and effective diagnosis of oncological diseases. Among the various possibilities current research in the world, the radiopharmaceuticals to chemotherapeutic base may be the most effective in detecting tumors, particularly Gastrointestinal Stromal Tumor (GIST), the Metastatic Renal Cell Carcinoma and neuroendocrine pancreatic tumors. However, difficulties in directing, as well as adhesion of the radiopharmaceutical in the desired location, are currently the main problems in the early detection and treatment of some of these tumors. Advances in the field of nanotechnology, particularly in recent years, indicate significant contribution to overcoming these obstacles, particularly in the implementation of molecular barriers as well as the functionalization of the nanoparticles, thereby improving targeting by the use of surface nucleotides, and the increased adhesion, which facilitates the release of the drug and therefore increases the chances of early diagnosis and more effective treatment. This study aimed to the production, characterization and evaluation of cytotoxicity, as well as in vivo biodistribution test Bevacizumab nanoparticles labeled with Technetium-99m radionuclide for detection of type GIST tumors. Bevacizumab was encapsulated in the form of nanoparticles by the emulsification method using double poly-acetic acid and polyvinyl alcohol polymers (PLA / PVA) at a concentration of 2% of the monoclonal antibody. The characterization of the nanoparticles was performed by the technique of scanning electron microscopy (SEM). The cytotoxicity assessment was performed by XTT assay with various cell lines of solid tumor cells. The labeling with technetium-99m was done by the direct method, and its yield determined by paper chromatography using paper Whatmam 1 as the stationary phase and acetone as mobile phase. In the biodistribution study

  13. Localization of parathyroid enlargement: experience with technetium-99m methoxyisobutylisonitrile and thallium-201 scintigraphy, ultrasonography and computed tomography

    Energy Technology Data Exchange (ETDEWEB)

    Geatti, O. (Ist. di Medicina Nucleare, Ospedale Civile, Udine (Italy)); Shapiro, B. (Div. of Nuclear Medicine, Michigan Univ., Ann Arbor, MI (United States)); Orsolon, P.G. (Ist. di Medicina Nucleare, Ospedale Civile, Udine (Italy)); Proto, G. (Endocrinologia, Ospedale Civile, Udine (Italy)); Guerra, U.P. (Ist. di Medicina Nucleare, Ospedale Civile, Udine (Italy)); Antonucci, F. (Nefrologia, Ospedale Civile, Udine (Italy)); Gasparini, D. (Ist. di Radiologia 2, Ospedale Civile, Udine (Italy))

    1994-01-01

    Technetium-99m methoxyisobutylisonitrile (MIBI), like thallium-201, has recently been introduced as a myocardial perfusion agent and is now also showing very promising results in parathyroid scintigrapy. The results of [sup 201]Tl/[sup 99m]Tc-pertechnetate and [sup 99m]Tc/MIBI/[sup 99m]Tc-pertechnetate subtraction scintigraphy, ultrasonography and computed tomography are presented in a series of 43 patients operated on for hyperparathyroidism. All four imaging modalities were confirmed to be reliable, scintigraphy being the most accurate. Sensitivities ranged from 81% to 95%, that of [sup 99m]Tc-MIBI being the highest. Moreover this tracer, which has more favourable physical and also biochemical properties, yielded images of superior quality. This allowed localization of the lesion by visual inspection only is as many as 86% of the patients with positive [sup 99m]Tc-MIBI/n9[sup 9m]Tc-pertechnetate subtraction scintigraphy. We believe that the higher sensitivity, superior image quality and lower cost of [sup 99m]Tc-MIBI imaging will make [sup 99m]Tc-MIBI the new radiopharmaceutical of choice for parathyroid scintigraphy (when one takes into account) the stability of labelling with large activities it is possible to perform three or four cardiac studies together with one parathyroid scintigraphic examination using one lyophililzed vial. (orig.)

  14. Sensitivity of [Tc-99m]methoxyisobutylisonitrile scan in patients with metastatic differentiated thyroid cancer

    NARCIS (Netherlands)

    Ronga, G.; Ventroni, G.; Montesano, T.; Filesi, M.; Ciancamerla, M.; Di Nicola, A. D.; Travascio, L.; Viestri, A. R.; Signore, A.

    2007-01-01

    Aim. The aim of the present study was to evaluate the diagnostic sensitivity of [Tc-99m]methoxyisobutylisonitrile ([Tc-99m]MIBI) in a large series of patients with metastatic differentiated thyroid carcinoma (DTC), as compared with I-131-whole body scan (WBS) and other diagnostic imaging techniques.

  15. Synthesis, labeling with {sup 99m}Tc and biokinetics of brains scintigraphy diaminodithiol perfusion radiopharmaceuticals; Sintese, marcacao com {sup 99m}Tc e biocinetica de radiofarmacos perfusorios diaminoditiolicos para cintilografias cerebrais

    Energy Technology Data Exchange (ETDEWEB)

    Goncalves, Marcos Moises

    1999-07-01

    The recent tomography status using radiopharmaceuticals have been contributing greatly with the 'age of certainty' in the diagnosis examination of syndromes, pathologies and clinical signs, because they can evidence some phenomena occurring in a molecular manner. The purpose of this work have had the development of new diaminodithiol (DADT) perfusion radiopharmaceuticals to be used in brain diagnosis using S.P.E.T. (Single Photon Emission Tomography). Initially, the rational planning had been performed with the new DADT molecular structures as radiopharmaceutical candidates. Using of Q.S.A.R. (Quantitative Structure Activity Relationship) techniques, the molecular descriptors such as partition coefficient and effective polarizability, have been studied in order to increase the blood brain barrier transport and the brain uptake respectively. Applying the Q.S.P.R. (Quantitative Structure Property Relationship) concepts to perform drug latentiation, based on bio-labile functional groups, the congener DADT derivative has been transformed into a pro-drug that works as a DADT moiety carrier, allowing the increasing of brain radiopharmaceutical uptake. Later on, synthetic routes and chemical purifications have been developed allowing the creation of the proposed chemical structure. Each new DADT derivative has been synthesized and analyzed in terms of elemental analysis, infrared and NMR spectra, in order to confirm its proposed chemical structure. Then, the new derivative has been labeled with {sup 99m}Tc, radiochemically purified, intravenously injected in Swiss mice, allowing its biodistribution to evidence its brain transport and uptake. The rational planning studies have been re-evaluated after each biodistribution had been performed, to see what kind of molecular descriptor was responsible for causing a stronger optimization in the brain perfusion characteristics and then, new DADT derivatives have been prepared. Three new DADT derivatives have been

  16. Technetium-99m tetrofosmin imaging in thyroid diseases: comparison with Tc-99m-pertechnetate, thallium-201 and Tc-99m-methoxyisobutylisonitrile scans

    Energy Technology Data Exchange (ETDEWEB)

    Klain, M. [Centro di Studio per la Medicina Nucleare, Consiglio Nazionale delle Ricerche (CNR) Napoli (Italy)]|[Univ. di Napoli (Italy). Medicina Nucleare; Maurea, S. [Medicina Nucleare, Istituto Nazionale dei Tumori, Napoli (Italy); Cuocolo, A. [Centro di Studio per la Medicina Nucleare, Consiglio Nazionale delle Ricerche (CNR) Napoli (Italy)]|[Univ. di Napoli (Italy). Medicina Nucleare; Colao, A. [Endocrinologia, Centro di Studio per la Medicina Nucleare del Consiglio Nazionale delle Ricerche (CNR), Napoli (Italy)]|[Univ. di Napoli (Italy); Marzano, L. [Endocrinologia, Centro di Studio per la Medicina Nucleare del Consiglio Nazionale delle Ricerche (CNR), Napoli (Italy)]|[Univ. di Napoli (Italy); Lombardi, G. [Endocrinologia, Centro di Studio per la Medicina Nucleare del Consiglio Nazionale delle Ricerche (CNR), Napoli (Italy)]|[Univ. di Napoli (Italy); Salvatore, M. [Centro di Studio per la Medicina Nucleare, Consiglio Nazionale delle Ricerche (CNR) Napoli (Italy)]|[Univ. di Napoli (Italy). Medicina Nucleare

    1996-12-01

    In this study, tetrofosmin whole-body scintigraphy was performed in 35 patients with evidence of thyroid diseases. All patients underwent laboratory evaluation of thyroid function as well as {sup 99m}Tc pertechnetate scan, thallium-201 {sup 99m}Tc-methoxyisobutylisonitrile (MIBI) whole-body studies. Thyroid images were semi-quantitatively analysed by a 4-point score: 0=no significant uptake; 1=uptake increased but inferior to normal thyroid tissue; 2=uptake equal to normal thyroid tissue; 3=uptake superior to normal thyroid tissue. A total of 41 thyroid nodules were detected, of which 15 were goitre nodules, 13 adenomas and 13 malignant lesions. In goitre nodules, concordant results of tetrofosmin and pertechnetate uptake (score 1 or 0) were observed in the majority of lesions (87%). In function adenomas both tetrofosmin uptake and pertechnetate uptake were score 3. In non-function adenomas tetrofosmin uptake was score 3, while pertechnetate uptake was score 0. In six malignant lesions, tetrofosmin uptake was score 3, while pertechnetate uptake was score 0; in the other seven lesions, where a prevalence of goitre abnormalities was observed, results of tetrofosmin and pertechnetate uptake were similar (score 0 or 1). In (70%) patients with malignant nodules, whole-body tetrofosmin images showed increased abnormal uptake in a total of 28 extra-thyroid tumour sites, as subsequently confirmed by other techniques. When tetrofosmin images were compared to {sup 201}Tl and {sup 99m}Tc-MIBI scans, concordant results were observed in all cases. In conclusion, tetrofosmin imaging may be particularly useful to characterize and stage patients with malignant thyroid nodules; it shows similar results to thallium but provides better image quality. Comparable findings were observed between tetrofosmin and MIBI studies. Thus, tetrofosmin may be an alternative to thallium and MIBI in the aforementioned patients. (orig.). With 4 figs., 3 tabs.

  17. {sup 99m}Tc sestamibi imaging. Can it be a useful substitute for hepatobiliary scintigraphy in infantile jaundice?

    Energy Technology Data Exchange (ETDEWEB)

    Sadeghi, R.; Kakhki, V.R.D.; Zakavi, R. [Mashhad Univ. of Medical Sciences (Iran). Nuclear Medicine Dept.; Kianifar, H.R. [Mashhad Univ. of Medical Sciences (Iran). Paediatric Dept.; Ansari, K. [Tehran Univ. of Medical Sciences (Iran). Nuclear Medicine Dept.

    2009-07-01

    Hepatobiliary scintigraphy is an integral part in the diagnostic work-up of the neonatal cholestasis syndrome. However, less than optimal specificity is its major disadvantage. Differentiation between biliary atresia and neonatal hepatitis is nearly impossible in some cases with poor hepatocellular function. {sup 99m}Tc sestamibi (MIBI) is a cationic lipophilic agent which is a substrate of P-glycoprotein. This glycoprotein is normally expressed in biliary canalicular surfaces of hepatocytes. This property provides a hepatic excretory mechanism which is different from bilirubin excretion. In this study we evaluated the value of {sup 99m}Tc MIBI in differential diagnosis of neonatal cholestasis. 20 infants with a mean age of 2.41 months (range, 0.1-5 months) were included in the study. Ten infants turned out to have extrahepatic biliary atresia and the other ten had neonatal hepatitis. Hepatobiliary (with {sup 99m}Tc BrIDA) and {sup 99m}Tc MIBI scintigraphy were performed for all the patients. {sup 99m}Tc MIBI scintigraphy has shown bowel activity in all patients, including the patients with biliary atresia. Hepatobiliary scintigraphy revealed bowel activity only in five patients with neonatal hepatitis. Bowel visualization with {sup 99m}Tc MIBI may be seen in patients with biliary atresia and {sup 99m}Tc MIBI has limited value in differential diagnosis of neonatal cholestasis. (orig.)

  18. Comparative uptake of Tc-99m sestamibi and Tc-99m tetrofosmin in cancer cells and tissue expressing P-Glycoprotein or multidrug resistance associated protein

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Jung Ah; Lee, Jae Tae; Yoo, Jung Ah [School of Medicine, Kyungpook National University, Daegu (Korea, Republic of)] (and others)

    2005-02-15

    {sup 99m}Tc-sestamibi(MIBI) and {sup 99m}Tc-tetrofosmin have been used as substrates for P-glycoprotein (Pgp) and multidrug resistance associated protein (MRP), which are closely associated with multidrug resistance of the tumors. To understand different handling of radiotracers in cancer cell lines expressing Pgp and MRP, we compared cellular uptakes of {sup 99m}Tc-MIBI and {sup 99m}Tc-tetrofosmin. The effects of cyclosporin A (CsA), well-known multidrug resistant reversing agent, on the uptake of both tracers were also compared. HCT15/CL02 human colorectal cancer cells for Pgp expressing cells, and human non-small cell lung cancer A549 cells for MRP expressing cells, were used for in vitro and in vivo studies. RT-PCR, western blot analysis and immunohistochemistry were used for detection of Pgp and MRP. MDR-reversal effect with CsA was evaluated at different drug concentrations after incubation with MIBI or tetrofosmin. Radioactivities of supernatant and pellet were measured with gamma well counter. Tumoral uptake of the tracers were measured from tumor bearing nude mice treated with or without CsA. RT-PCR, western blot analysis of the cells and immunochemical staining revealed selective expression of Pgp and MRP for HCT15/CL02 and A549 cells, respectively. There were no significant difference in cellular uptakes of both tracers in HCT15/CL02 cells, but MIBI uptake was slightly higher than that of tetrofosmin in A549 cells. Co-incubation with CsA resulted in a increase in cellular uptakes of MIBI and tetrofosmin. Uptake of MIBI or tetrofosmin in HCT15/CL02 cells was increased by 10-and 2.4-fold, and by 7.5 and 6.3-fold in A549 cells, respectively. Percentage increase of MIBI was higher than that of tetrofosmin with CsA for both cells ({rho} < 0.05). In vivo biodistribution study showed that MIBI (114% at 10 min, 257% at 60 min, 396% at 24C min) and tetrofosmin uptake (110% at 10 min, 205% at 60 min, 410% at 240 min) were progressively increased by the time, up to

  19. Usefulness of {sup 99m}Tc-methoxy-isobutyl-isonitrile scintigraphy for preoperative localization of adenoma in primary hyperparathyroidism

    Energy Technology Data Exchange (ETDEWEB)

    Yamaguchi, Seiji; Kobayashi, Yoshiyuki; Tsujikawa, Kozo [Ikeda Municipal Hospital, Osaka (Japan)] (and others)

    2001-09-01

    We evaluated the usefulness of {sup 99m}Tc-methoxy-isobutyl-isonitrile (MIBI) dual phase scintigraphy for detecting hyperfunctioning parathyroid adenoma. We retrospectively reviewed 18 hyperparathyroid patients who received MIBI prior to neck exploration and compared the radiological findings of MIBI with ultrasonography (US) and magnetic resonance imaging (MRI). Fifteen patients were studied with MRI, and 17 patients were examined with US. All patients were found to have a solitary parathyroid adenoma histopathologically. MIBI correctly revealed the location of 17 adenomas among 18 confirmed tumors. In our series, there was one false-positive case that was found to have thyroid adenoma. The diagnostic sensitivity of MIBI MRI and US and 94.4%, 80% and 52.5%, respectively. The positive predictive value (PPV) was 94.4% for MIBI, 81.8% for MRI and 92.3% for US. We conclude that MIBI is useful and accurate for the preoperative localization of adenoma in primary hyperparathyroidism. (author)

  20. Usefulness of 99mTc-methoxy-isobutyl-isonitrile scintigraphy for preoperative localization of adenoma in primary hyperparathyroidism

    International Nuclear Information System (INIS)

    We evaluated the usefulness of 99mTc-methoxy-isobutyl-isonitrile (MIBI) dual phase scintigraphy for detecting hyperfunctioning parathyroid adenoma. We retrospectively reviewed 18 hyperparathyroid patients who received MIBI prior to neck exploration and compared the radiological findings of MIBI with ultrasonography (US) and magnetic resonance imaging (MRI). Fifteen patients were studied with MRI, and 17 patients were examined with US. All patients were found to have a solitary parathyroid adenoma histopathologically. MIBI correctly revealed the location of 17 adenomas among 18 confirmed tumors. In our series, there was one false-positive case that was found to have thyroid adenoma. The diagnostic sensitivity of MIBI MRI and US and 94.4%, 80% and 52.5%, respectively. The positive predictive value (PPV) was 94.4% for MIBI, 81.8% for MRI and 92.3% for US. We conclude that MIBI is useful and accurate for the preoperative localization of adenoma in primary hyperparathyroidism. (author)

  1. Postreperfusion myocardial technetium-99m-sestamibi defect corresponds to area at risk

    Energy Technology Data Exchange (ETDEWEB)

    Poulsen, Runa Hyldgaard, E-mail: runa.poulsen@ki.au.dk [Clinical Institute, Aarhus University, DK-8000 Aarhus C (Denmark); Botker, Hans Erik [Department of Cardiology, Aarhus University Hospital, Skejby (Denmark); Rehling, Michael [Department of Nuclear Medicine, Aarhus University Hospital, Skejby (Denmark)

    2011-08-15

    Introduction: Technetium-99m-sestamibi (MIBI) is the most frequently used myocardial perfusion tracer in patients with ischemic heart disease. In patients with acute ST-elevation myocardial infarction, we previously found that the defect in myocardial MIBI uptake was the same in patients injected with MIBI before primary angioplasty and in patients injected immediately after successful treatment. Thus, reperfusion may not be followed by increased uptake of MIBI. Instead, the MIBI defect after reperfusion may reflect the area at risk (AAR) defined by MIBI injected before treatment. We intended to investigate whether myocardial imaging with MIBI administered after reperfusion reflects myocardial perfusion or rather the ischemic AAR. Methods: In 12 pigs, left anterior descending coronary artery was totally occluded for 45 min with an angioplasty balloon. After a 2-h reperfusion, MIBI was injected intravenously, and {sup 153}Gd-microspheres were injected in left atrium. AAR and infarct size (IS) were determined by histochemical staining. MIBI and microsphere distribution were evaluated by counting the sliced left ventricle on a gamma camera. Defects were defined as uptake less than 45% of maximum uptake. Results: The mean{+-}S.D. defect size as a fraction of left ventricle was for MIBI 21%{+-}5.5%, AAR 25%{+-}6.3%, IS 13%{+-}3.9% and microspheres defect size 7.3%{+-}5.5%. MIBI defect size overestimated IS (P=.0005) and microspheres defect size (P=.0001), but it was not significantly different from AAR (P=.30). Conclusion: In a porcine model of myocardial infarction after 45 min of ischemia, MIBI administered 120 min after reperfusion delineates AAR.

  2. Development of MIBI kit for heart imaging

    International Nuclear Information System (INIS)

    99mTc-isonitriles have been shown to be a very promising substitute for Thallium-201 (201TI) for myocardial perfusion imaging. In this study, the lyophilized kit of Methoxyisobutylisonitrile (MIBI) was prepared and labeled with 99mTc. Several factors affecting the labeling yield such as the kit's stannous content, boiling time during labeling, and the volume of 99mTc used during reconstitution were also investigated. The radiochemical purity (RCP) determination of the labeled product was analyzed by HPLC, solvent-extraction, TLC and ITLC-SG chromatographic methods in various systems. Animal biodistribution study performed in rats indicated the 99mTc-MIBI accumulation in the myocardial is up to 3 hours with little or no redistribution. Toxicity studies performed indicate no clinical signs of abnormality in mice at injected dose equivalent in amount of 100 times the human dose in proportion to body weight. Stability studies of the labeled complex performed at room temperature showed no change in radiochemical purity (> 95%) 6 hours post-preparation. Compatibility and comparative studies were done using both MINT and commercially available MIBI kits and 99mTc generator eluates. From the results obtained the MINT produced MIBI kits were found to be comparable in quality to that of commercials. (author)

  3. Comparison of the accumulation and efflux kinetics of technetium-99m sestamibi and technetium-99m tetrofosmin in an MRP-expressing tumour cell line

    International Nuclear Information System (INIS)

    The potential clinical use of technetium-99m labeled sestamibi (Tc-MIBI) and tetrofosmin (Tc-Tfos) to image tumours is currently being evaluated. In this study, the accumulation and efflux of Tc-MIBI and Tc-Tfos in the nasopharyngeal carcinoma cell line CNE-1 were examined in the presence or absence of various inhibitors of P-glycoprotein (PGP) and/or multidrug resistance associated protein (MRP) activity [GG918, PSC833, verapamil (Vrp), cyclosporin A (CsA) and buthionine sulfoximine (BSO)]. Reverse-transcriptase polymerase chain reaction analysis and immunodetection of the CNE-1 cells detected expression of MRP, MRP1 and MRP2 but not PGP. Tc-MIBI and Tc-Tfos accumulation was increased (P2 times greater than for Tc-MIBI). However, no qualitative differences in inhibitors were seen between Tc-MIBI and Tc-Tfos. These results suggest that both Tc-MIBI and Tc-Tfos are substrates for the MRP transporter and that PSC833, Vrp, CsA and BSO but not GG918 can inhibit MRP activity. These results indicate that Tc-MIBI and Tc-Tfos may be suitable imaging agents for detecting MRP-mediated drug resistance in human cancers. (orig.)

  4. Technetium-99m ceftizoxime kit preparation

    Directory of Open Access Journals (Sweden)

    Simone Odília Fernandes Diniz

    2005-10-01

    -99m Ceftizoxima (99mTc-CFT, com estabilidade e atividade biológica preservadas, capaz de identificar um foco séptico (E.coli em um modelo experimental de infecção em ratos. A preparação do kit de CFT baseou-se em uma mistura de soluções contendo o antibiótico ceftizoxima (2,5mg/mL e o agente redutor ditionito de sódio (6,0mg/mL que foram submetidos a um processo de liofilização. Após a liofilização, o kit foi reconstituído with 1,0 mL de solução de pertecnetato de sódio (Na99mTcO4 -, contendo uma atividade de 370 MBq. Em seguida, a solução foi incubada, por 10 min, em banho fervente (1000C e, posteriormente, foi resfriada em água corrente por 5 min. A eficiência de marcação foi da ordem de 92% permanecendo estável por 6 horas e o kit permaneceu estável por 2 meses. A atividade biológica do 99mTc-CFT foi avaliada por difusão em ágar impregnado com E.coli e S. aureus. Foram utilizados 07 ratos Wistar, pesando entre 200 a 250 g, para o desenvolvimento do foco séptico. Após 24 horas da indução do foco infeccioso (E.coli, os animais foram anestesiados e 0,1 mL da 99mTc-CFT (37 MBq foi injetado na veia da cauda dos animais. As imagens de 1, 2 e 6 horas após a injeção foram adquiridas em uma gama câmara e as regiões de interesse (ROIS foram calculadas. Os valores obtidos dos diâmetros dos halos de inibição para 99mTc-CFT foram 27,16±0,23 e 27,17±0,20 para S. aureus e E.coli, respectivamente, sendo que para CFT não marcada foram 30,4±0,33 e 29,43±0,26, respectivamente. Os resultados obtidos da biodistribuição da 99mTc-CFT nos animais com focos infecciosos mostraram uma relação alvo/não alvo de 1,97±0,31, 2,10±0,42 e 2,01±0,42 para os tempos de 1, 2 e 6 horas, respectivamente. As imagens obtidas mostraram nítida captação do antibiótico marcado (99mTc-CFT pelo foco infeccioso ao longo do experimento. Os resultados obtidos neste trabalho atestam a viabilidade de produção de um kit da ceftizoxima marcada com 99m tecn

  5. Bone marrow uptake of 99mTc-sestamibi in patients with multiple myeloma and related gammopathies

    International Nuclear Information System (INIS)

    Aim: The aim of this study was to assess the diagnostic value of 99mTc-sestamibi (MIBI) in the detection on bone marrow involvement in patients suffering from multiple myeloma (MM) or monoclonal gammopathy of undefined significance. 99mTc-Sestamibi is routinely used in the diagnostic approach of several neoplastic diseases and a MIBI scan was recently proposed as a diagnostic tool for staging and monitoring the therapy in multiple myeloma. Materials and methods: 68 pts with MM and 82 pts with monoclonal gammopathy of undetermined significance (MGUS) were enrolled in this study. Forty nine out sixty eight MM pts had active disease (AD), 11/68 were in complete remission (CR) and 8/68 in partial remission (PR) after chemotherapy. Diagnosis of the disease was made according to standard criteria (Greipp, 1992). The clinical status at time of the scan was assessed by complete clinical and biochemical evaluations including full blood counts, renal and liver function tests, protein electrophoresis and evaluation of monoclonal component, serum Ig concentration, CRP, 2-microglobulin, urinary light chain and bone marrow biopsy. In all the pts a whole-body scans were obtained 10 min after the i.v. injection of 740 MBq of 99mTc-MIBI in anterior and posterior view. All the scans were scored semi quantitatively according to extension and intensity of tracer uptake (Pace, 1998). Results: 81 out 82 MGUS pts had a negative 99mTc-MIBI scan. One MGUS patient showed a mild diffuse pathologic uptake in bone marrow of the thighs. As to the MM pts, 54/68 pts (47 with AD, 8 with PR and 1 with CR) had a positive 99mTc-MIBI scan, while the 99mTc-MIBI scan was negative in 14/68 pts (10 with CR, 2 with PR and 2 with AD). The radioactivity ratios of the more evident bone marrow involvement or soft tissue uptake to a contralateral normal tissue (T/N) were calculated in AD patients(T/N=2.06 0.5). The overall sensitivity of the 99m Tc-MIBI scintigraphy was 92% while specificity was 95% in overall

  6. Padronização do método para cálculo da captação renal absoluta do99mTc-DMSA em cria Standardization of a method to calculate absolute renal uptake of 99mTc-DMSA in children

    Directory of Open Access Journals (Sweden)

    Carla Rachel Ono

    2006-02-01

    Full Text Available OBJETIVO: O trabalho teve por objetivo padronizar o método e estabelecer valores normais da captação renal absoluta do99mTc-DMSA em crianças. MATERIAIS E MÉTODOS: Vinte e duas crianças (idade de 7 meses a 10 anos; média de 4,5 anos sem doença renal prévia foram submetidas a cintilografia renal estática com 99mTc-DMSA. Dezoito apresentavam ultra-sonografia, uretrocistografia miccional, "clearance" de creatinina e padrão visual da cintilografia renal estática normais. Quatro crianças foram excluídas por não terem completado ou por apresentarem redução do "clearance" de creatinina. A captação absoluta de DMSA (DMSA-Abs foi calculada como a porcentagem da atividade administrada retida em cada rim após seis horas da administração do radiofármaco. RESULTADOS: Os valores de DMSA-Abs foram de 21,8% ± 3,2% para o rim direito e de 23,1% ± 3,3% para o rim esquerdo. Os valores da captação absoluta não mostraram correlação com a idade dos pacientes estudados, apesar da tendência de aumento do "clearance" de creatinina com a idade. CONCLUSÃO: A definição de valores normais da DMSA-Abs permite o emprego deste parâmetro na avaliação inicial e acompanhamento de doenças renais, principalmente em pacientes com acometimento bilateral ou com rim único (nos quais a função diferencial direita X esquerda tem valor limitado.OBJECTIVE: To standardize a method and determine normal values for absolute renal uptake of 99mTc-DMSA in children with normal creatinine clearance. MATERIALS AND METHODS: Twenty-two children (between 7 months and 10 years of age; mean 4.5 years without clinical evidence of renal disease were studied using 99mTc-DMSA scintigraphy. Eighteen had normal renal ultrasonography, micturating urethrocystography, creatinine clearance and visual interpretation of the scintigraphy with 99mTc-DMSA. Four children were excluded, one with incomplete creatinine clearance and three due to reduction in the creatinine clearance

  7. Estudos in vitro e in vivo de análogo da timidina marcada com complexo organometálico de tecnécio-99m para potencial uso em diagnóstico tumoral Studies in vitro and in vivo of thymidine analog labeled with organometalic complex of technetium-99m for potential use in tumor diagnosis

    Directory of Open Access Journals (Sweden)

    Rodrigo Luis Silva Ribeiro Santos

    2008-03-01

    Full Text Available Análogos da timidina têm sido marcados com diferentes radioisótopos devido ao seu potencial em monitorar a proliferação incontrolável de células. Considerando que o radioisótopo tecnécio-99m ainda mantém uma posição privilegiada devido às suas propriedades químicas e nucleares, este trabalho constituiu-se no desenvolvimento da marcação da timidina com o 99mTc, mediante o emprego de compostos organometálicos. Os objetivos principais foram a síntese do precursor carbonil-tecnécio-99m, marcação da timidina com este precursor, estudo da estabilidade, e avaliações radioquímicas e biológicas com animais sadios e portadores de tumor. A síntese do precursor organometálico e a marcação da timidina com este precursor foi realizada com > 97% e > 94% de pureza radioquímica, respectivamente, obtendo-se também uma boa estabilidade em até 6 h em temperatura ambiente. A transquelação frente aos aminoácidos cisteína e histidina apresentou perdas entre 8 e 11% para concentrações de até 300 mM. Os ensaios de biodistribuição em camundongos sadios indicaram que o complexo radiomarcado apresentou um rápido depuramento sangüíneo e baixa captação nos demais órgãos, com predominância de excreção da droga pelo sistema urinário e hepatobiliar. A captação tumoral foi de 0,28 e 0,18 %DI/g para tumor de pulmão e mama, respectivamente. Os resultados obtidos sugerem maiores investigações em outros análogos da timidina.Thymidine analogs have been labeled with different radioisotopes due to their potential in monitoring the uncontrollable cell proliferation. Considering that the radioisotope technetium-99m still keeps a privileged position as a marker due to its chemical and nuclear properties, this work was designed to develop a new technique of labeling of thymidine analog with 99mTc, by means of the organometallic compounds. The aims of this research were: synthesis of the organometallic precursor technetium-99m

  8. A comparative study of technetium-99m sestamibi and technetium-99m tetrofosmin single-photon tomography in the detection of nasopharyngeal carcinoma

    International Nuclear Information System (INIS)

    The intention of this prospective study was to compare the diagnostic potential of technetium-99m sestamibi (MIBI) and a novel radiotracer, 99mTc-Tetrofosmin (Tetro), for the assessment of primary nasopharyngeal carcinoma (NPC) and the differentiation of residual disease from post-therapy changes. A total of 38 patients underwent MIBI and Tetro single-photon emission tomography (SPET) imaging at initial presentation (n=22) or following therapy (n=16). The findings were correlated with computed tomography or magnetic resonance imaging (MRI) on a site-by-site basis. Tumour/background (Tm/Bkg) ratios were obtained on coronal sections. Biopsy (nine patients) and/or 12- to 24-month clinical follow-up data were available in the post-therapy group. All primary disease sites were accurately detected by both imaging studies. Although there was no statistical difference between the two imaging techniques in the detection of primary disease, MIBI was superior to Tetro in the detection of regional lymph node metastases (sensitivity: 95% vs 79%). Tetro and MIBI SPET were true-positive in all patients (n=7) with proven residual/recurrent disease. In nine patients who had no evidence of residual/recurrent tumour, MRI was false-positive in five while Tetro and MIBI SPET were false-positive in two and three patients, respectively. Tm/Bkg ratios were ≤1.7 in all false-positive cases except one. Tetro, MIBI and MRI had specificities of 78%, 67% and 44%, and accuracies of 87.5%, 81% and 69%, respectively. The results of Tetro and of MIBI SPET were not statistically different from one another with regard to the prediction of residual/recurrent or metastatic NPC. (orig.). With 3 figs., 4 tabs

  9. Tc-99m imaging agents

    International Nuclear Information System (INIS)

    A wide range of pharmaceuticals for labeling with Tc-99m, developed by the Soreq Radiopharmaceuticals Department, is described. Details of the production and quality control of 13 kits are given, as well as the range of results required for consistently high quality imaging agents

  10. Technetium-99m methoxyisobutylisonitrile imaging for parathyroid adenoma: relationship to P-glycoprotein or multidrug resistance-related protein expression

    International Nuclear Information System (INIS)

    Gland size has been reported to have a major influence on localisation of parathyroid adenomas by technetium-99m methoxyisobutylisonitrile (99mTc-MIBI) imaging. It has also been suggested that P-glycoprotein (Pgp) expression in parathyroid adenomas may influence localisation because false negative studies have been reported with large tumours and true positives with very small tumours. Therefore, the purpose of this study was to retrospectively evaluate the relationship between 99mTc-MIBI parathyroid imaging results and Pgp or multidrug resistance-related protein (MRP) expression in parathyroid adenomas. Before surgery, 47 patients with large parathyroid adenomas (larger than 1.5 g) underwent early and delayed parathyroid imaging, 10 min and 2 h after intravenous injection of 99mTc-MIBI. Immunohistochemical analyses (IHA) were performed, using multiple non-consecutive sections of the operative specimens, to detect Pgp or MRP expression. According to the results of IHA, the 34 parathyroid adenomas were separated into four groups: (1) three adenomas positive for both Pgp and MRP expression, (2) one adenoma positive for Pgp but negative for MRP expression, (3) four adenomas negative for Pgp but positive for MRP expression and (4) 39 adenomas with negative for both Pgp and MRP expression. All 39 adenomas in group 4 could be detected by 99mTc-MIBI parathyroid imaging. None of the eight adenomas in groups 1-3 could be detected by 99mTc-MIBI parathyroid imaging (P99mTc-MIBI imaging in localising parathyroid adenomas preoperatively. (orig.)

  11. Comparison of the accumulation and efflux kinetics of technetium-99m sestamibi and technetium-99m tetrofosmin in an MRP-expressing tumour cell line

    Energy Technology Data Exchange (ETDEWEB)

    Utsunomiya, K.; Su, Z.-F.; Ichise, M. [Nuclear Medicine, Mount Sinai Hospital, University of Toronto, Ont. (Canada); Ballinger, J.R. [Nuclear Medicine, Addenbrooke' s Hospital, Cambridge University, Cambridge (United Kingdom); Piquette-Miller, M.; Tang, W. [Faculty of Pharmacy, University of Toronto, 19 Russell Street, Toronto, ON (Canada); Rauth, A.M. [Division of Experimental Therapeutics, Princess Margaret Hospital, Ontario Cancer Institute, ON (Canada)

    2000-12-01

    The potential clinical use of technetium-99m labeled sestamibi (Tc-MIBI) and tetrofosmin (Tc-Tfos) to image tumours is currently being evaluated. In this study, the accumulation and efflux of Tc-MIBI and Tc-Tfos in the nasopharyngeal carcinoma cell line CNE-1 were examined in the presence or absence of various inhibitors of P-glycoprotein (PGP) and/or multidrug resistance associated protein (MRP) activity [GG918, PSC833, verapamil (Vrp), cyclosporin A (CsA) and buthionine sulfoximine (BSO)]. Reverse-transcriptase polymerase chain reaction analysis and immunodetection of the CNE-1 cells detected expression of MRP, MRP1 and MRP2 but not PGP. Tc-MIBI and Tc-Tfos accumulation was increased (P<0.0001) and efflux decreased (P<0.05) in the presence of BSO, CsA, Vrp and PSC833 but not GG918, which is a specific inhibitor of PGP. The absolute accumulation of Tc-MIBI was approximately twofold higher than that seen with Tc-Tfos, whereas the addition of inhibitors caused a much greater suppression of Tc-Tfos transport (>2 times greater than for Tc-MIBI). However, no qualitative differences in inhibitors were seen between Tc-MIBI and Tc-Tfos. These results suggest that both Tc-MIBI and Tc-Tfos are substrates for the MRP transporter and that PSC833, Vrp, CsA and BSO but not GG918 can inhibit MRP activity. These results indicate that Tc-MIBI and Tc-Tfos may be suitable imaging agents for detecting MRP-mediated drug resistance in human cancers. (orig.)

  12. Hypoxia-induced alteration of tracer accumulation in cultured cancer cells and xenografts in mice: implications for pre-therapeutic prediction of treatment outcomes with 99mTc-sestamibi, 201Tl chloride and 99mTc-HL91

    International Nuclear Information System (INIS)

    Weak visualization of tumours in pre-therapeutic scintigrams with technetium-99m sestamibi (MIBI) is likely a predictive sign of unfavourable tumour response to radiotherapy and chemotherapy. However, factors relating to this scintigraphic finding are not well understood. The presence of hypoxic tumour cells is one of the major reasons for therapeutic failure; consequently, we attempted to determine whether oxygenation status affects 99mTc-MIBI accumulation in tumour cells. LS180 human colon cancer and T24 human bladder cancer cells were incubated in air or N2 gas at 37 C. Cellular uptake of 99mTc-MIBI was subsequently determined at 15, 60 and 120 min. Uptake of thallium-201 chloride was also assessed. Uptake of 99mTc-HL91 was assessed as a hypoxic marker. Accumulation of the tracers in LS180 xenografts was observed in mice treated with 5 mg/kg hydralazine and compared with that in untreated mice. pO2 in the medium and tumours was measured with O2 microelectrodes. N2 gas flow gradually reduced pO2 in the cell suspension to 1-2 mmHg in 60 min. Cellular uptake of 99mTc-MIBI in LS180 cells decreased by approximately 30% in N2 gas in comparison to that in air throughout the study. Hypoxia had a more prominent influence on 201Tl uptake, which displayed a reduction of approximately 60% in N2 gas at 120 min, than on 99mTc-MIBI uptake. On the other hand, N2 gas induced an increase of 170% in 99mTc-HL91 uptake at 120 min, indicating the hypoxic condition of cells. The results of in vitro assays employing the T24 cell line were similar to those obtained with the LS180 cell line. Hydralazine treatment markedly reduced 99mTc-MIBI and 201Tl accumulation in LS180 xenografts; moreover, intratumoural pO2 decreased from 14.5±6.6 mmHg to 7.6±6.2 mmHg. 99mTc-HL91 accumulation in xenografts was markedly increased by hydralazine. In conclusion, hypoxia reduced accumulation of 99mTc-MIBI and 201Tl in tumour cells. Accordingly, hypoxia may be an important factor in terms of the

  13. Technetium-99m methoxyisobutylisonitrile localizes an ectopic ACTH-producing tumour: case report and review of the literature

    Energy Technology Data Exchange (ETDEWEB)

    Jacobsson, H. (Dept. of Diagnostic Radiology, Karolinska Hospital, Stockholm (Sweden)); Wallin, G. (Dept. of Surgery, Karolinska Hospital, Stockholm (Sweden)); Werner, S. (Dept. of Endocrinology and Diabetology, Karolinska Hospital, Stockholm (Sweden)); Larsson, S.A. (Dept. of Hospital Physics, Karolinska Hospital, Stockholm (Sweden))

    1994-06-01

    Extensive investigation including whole-body examinations with computed tomography and magnetic resonance imaging did not detect the suspected ectopic ACTH-producing tumour in a patient with advanced Cushing's syndrome and hypokalemic alkalosis. Gamma camera examination with technetium-99m methoxyisobutylisonitrile (MIBI) depicted the tumour, which was localized in the anterior neck and mediastinum. This was later verified by surgery. [sup 99m]Tc-MIBI is normally used for myocardial scintigraphy. Its accumulation is unspecific and merely reflects metabolic activity. Despite this, the present case shows that examination with this agent can provide important information with regard to tumour localization in a given situation, thereby serving as a complement to other imaging modalities. The current literature on [sup 99m]Tc-MIBI for tumour diagnosis is reviewed. (orig.)

  14. MIBI-SPECT in hypofunctioning thyroid nodules for detection of thyroid carcinoma; MIBI-SPECT bei kalten Knoten zur Schilddruesenkarzinomdetektion

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, M.; Schicha, H. [Universitaetsklinikum Koeln (Germany). Klinik und Poliklinik fuer Nuklearmedizin

    2010-12-15

    The clinical usefulness of Tc-99m-MIBI in hypofunctioning thyroid nodules for detection of thyroid carcinoma is presented. Tc-99m-MIBI is a lipophilic cation and a non-specific radiopharmaceutical for tumour imaging. It has become an important imaging technique for the assessment of hypofunctioning thyroid nodules because of its high negative predictive value excluding malignant thyroid tumours. After injection of Tc-99m-MIBI either a single-phase protocol with late planar and SPECT images about 1-2 h post injection or a double-phase protocol with early (about 15-30 min p.i.) and late images (about 2 h p.i.) were reported. Findings include a reduced, an isointense or an increased Tc-99m-MIBI accumulation in the thyroid nodule in comparison to the paranodular thyroid tissue and in comparison to pertechnetate thyroid scintigraphy. A 'Match' between pertechnetate and Tc-99m-MIBI scintigraphy is a concordantly decreased uptake in the thyroid nodule in comparison to the normal thyroid gland. This finding has a negative predictive value of >97% to exclude differentiated thyroid cancer. A definite 'Mismatch' means a cold thyroid nodule on pertechnetate scintigraphy and an increased uptake of Tc-99m-MIBI in comparison to the MIBI-uptake of the paranodular thyroid tissue. The positive predictive value of this finding for malignancy varies between studies and is in the range of <10-65% (Cologne data: 19%) depending on the prevalence of malignant thyroid tumours in the patient population studied. An isointense uptake was not associated with thyroid malignancy according to 'Cologne' data. Further studies are desirable for better characterization of the method. (orig.)

  15. Property of electrocardiogram gated single photon emission tomography by sup 99m Tc-methoxy isobutyl isonitrile

    Energy Technology Data Exchange (ETDEWEB)

    Imai, Kamon; Nishio, Yukari; Araki, Yasushi; Saito, Satoshi; Ozawa, Yukio; Yasugi, Tadao; Hagiwara, Kazuo; Kamata, Rikisaburo (Nihon Univ., Tokyo (Japan). School of Medicine)

    1992-06-01

    {sup 99m}Tc-methoxy isobutyl isonitrile (MIBI) is a new developed myocardial perfusion imaging agent. Because this compound has higher photon energy than thallium (Tl), electrocardiogram gated single photon emission tomography (SPECT): end-diastolic (ED) and end-systolic (ES) short axis (SA) images could be taken. To investigate property of gated MIBI SPECT, MIBI myocardial scintigraphy, Tl scintigraphy (TMS) and analysis of left ventricular wall motion were performed in 6 patients with myocardial infarction. Left ventricule was divided into 8 segments. Perfusion defect (PD) was scored: '0' (normal), '1' (hypo-perfusion), '2' (defect). Wall motion abnormality (WMA) was also scored: '0' (normo-kinesis), '1' (hypo-kinesis), '2' (a-, dys-kinesis). Severity and extent of PD and WMA were calculated. Severity of WMA was 3.0{+-}2.0 (M{+-}SD), severity of PD was 3.3{+-}1.7 in TMS, 3.7{+-}1.3 in no-gated MIBI, 5.0{+-}0.6 in ES-MIBI, 7.3{+-}2.0 in ED-MIBI. Extent of WMA was 2.3{+-}1.0. Extent of PD was 2.5{+-}1.3 in TMS, 3.0{+-}1.6 in no-gated MIBI, 3.5{+-}0.8 in ES-MIBI, 4.8{+-}1.0 in ED-MIBI. Compared with wall motion abnormality, severity and extent of PD in ED-MIBI was larger. From our data, it is concluded that perfusion defect in ED-MIBI was overestimated significantly. When we evaluate gated MIBI image, we must consider this property. (author).

  16. Comparison of the uptakes of {sup 99m}Tc-sestamibi and {sup 99m}Tc-tetrofosmin in cancer cell lines expressing multidrug resistance

    Energy Technology Data Exchange (ETDEWEB)

    Yoo, Jeong Ah; Chung, Shin Young; Seo, Myeng Rang; Kwak, Dong Suk; Ahm, Byeong Cheol; Lee, Kyu Bo; Lee, Jae Tae [Kyungpook National University School of Medicine, Taegu (Korea, Republic of)

    2003-06-01

    Cellular uptakes of {sup 99m}Tc-sestamibi(MIBI) and {sup 99m}Tc-tetrofosmin into cancer cell lines expressing multidrug resistance(MDR) were investigated and compared. The effects of verapamil and cyclosporin A, well-known multidrug resistant reversing agents, on cellular uptakes of both tracers were also compared. Doxorubicin-resistant HCT15/CL02 human colorectal cell and doxorubicin-resistant K562(Adr) and vincristin-resistant K562(Vcr) human leukemic cells were studied. RT-PCR analysis was used for the detection of mdr1 mRNA expression. MDR-reversal effects with verapamil and cyclosporine A were evaluated at different drug concentrations after incubation with MIBI and tetrofosimin for 1, 15, 30, 45 and 60 min, using single-cell suspensions at 1x10{sup 6} cells/ml incubated at 37 .deg. C. Radioactivity in supernatants and pellets were measured with gamma well counter. The cellular uptakes of MIBI and tetrofosmin in K562(Adr) and K562(Vcr) were lower than those of parental K562 cell. In HCT15/CL02 cells ad K562(Adr) cells, there were no significant difference in cellular uptakes of both tracers, but cellular uptake of MIBI was higher than that of tetrofosmin in K562(Vcr) cells. Coincubation with verapamil resulted in a increase in cellular uptakes of MIBI and tetrofosmin. Verapamil increased cellular uptakes of MIBI and tetrofosmin by HCT15/LC02 cell by 11.9- and 6.8-fold, by K562(Adr) cell by 14.3- and 8-fold and by K562(Vcr) cell by 7- and 5.7-fold in maximum, respectively. Cyclosporin A increased cellular uptakes of MIBI and tetrofosmin by HCT15/CL02 cell by 10- and 2.4-fold, by K562(Adr) cell by 44- and 13-fold and by K562(Vcr) cell by 18.8- and 11.8-fold in maximum, respectively. Taking together, MIBI and tetrofosmin are considered as suitable radiopharmaceuticals for detecting multidrug resistance. However, MIBI seems to be a better tracer than tetrofosmin for evaluating MDR reversal effect of the modulators. Since cellular uptakes of both tracers might

  17. The role of Tc-99m sestamibi imaging in predicting clinical response to chemotherapy in lung cancer

    International Nuclear Information System (INIS)

    Multidrug resistance (MDR) is a major problem in lung cancer. Tc-99m methoxyisobutyl isonitrile (MIBI) has been demonstrated to be a non-invasive marker to diagnose MDR1 related P-glycoprotein (Pgp) and multidrug resistance-associated protein (MRP) expression in various solid tumors. The aim of this study was to evaluate the relationship between the degree of Tc-99m MIBI uptake and its retention on delayed images and the response to chemotherapy in lung cancer. Twenty-three patients (1 woman and 22 men, age range 40-67 years) with lung cancer (9 small cell and 14 non-small cell) were examined with Tc-99m MIBI imaging before chemotherapy. After i.v. administration of 740 MBq Tc-99m MIBI, planar and SPECT imaging at 30 minutes and 2 hours was performed. Tumor to normal lung uptake ratio (T/N) and percent retention were measured. Response to chemotherapy was evaluated according to follow-up CT and grouped as complete responders (CR), partial responders (PR) and non-responders (NR). Clinical follow-up and CT evaluation revealed that 12 patients had partial remission, 4 patients had complete remission and 7 patients had no-remission after chemotherapy. Statistically, there was no significant correlation between early (30 min), delayed (2 hr) T/N ratios and percent retention of Tc-99m MIBI with chemotherapeutic response of the lung cancer among the three groups (p>0.05). Results of the current study imply that Tc-99m MIBI uptake and the retention index may not correlate with chemotherapy response in lung cancer, so that the accuracy of this method needs to be verified in a larger series with additional investigation at the molecular level. (author)

  18. The role of Tc-99m sestamibi imaging in predicting clinical response to chemotherapy in lung cancer

    Energy Technology Data Exchange (ETDEWEB)

    Dirlik, A.; Burak, Z.; Goksel, T.; Erinc, R.; Karakus, H.; Ozcan, Z.; Veral, A.; Ozhan, M. [Ege Universitesi, Izmir (Turkey). Medical Faculty

    2002-04-01

    Multidrug resistance (MDR) is a major problem in lung cancer. Tc-99m methoxyisobutyl isonitrile (MIBI) has been demonstrated to be a non-invasive marker to diagnose MDR1 related P-glycoprotein (Pgp) and multidrug resistance-associated protein (MRP) expression in various solid tumors. The aim of this study was to evaluate the relationship between the degree of Tc-99m MIBI uptake and its retention on delayed images and the response to chemotherapy in lung cancer. Twenty-three patients (1 woman and 22 men, age range 40-67 years) with lung cancer (9 small cell and 14 non-small cell) were examined with Tc-99m MIBI imaging before chemotherapy. After i.v. administration of 740 MBq Tc-99m MIBI, planar and SPECT imaging at 30 minutes and 2 hours was performed. Tumor to normal lung uptake ratio (T/N) and percent retention were measured. Response to chemotherapy was evaluated according to follow-up CT and grouped as complete responders (CR), partial responders (PR) and non-responders (NR). Clinical follow-up and CT evaluation revealed that 12 patients had partial remission, 4 patients had complete remission and 7 patients had no-remission after chemotherapy. Statistically, there was no significant correlation between early (30 min), delayed (2 hr) T/N ratios and percent retention of Tc-99m MIBI with chemotherapeutic response of the lung cancer among the three groups (p>0.05). Results of the current study imply that Tc-99m MIBI uptake and the retention index may not correlate with chemotherapy response in lung cancer, so that the accuracy of this method needs to be verified in a larger series with additional investigation at the molecular level. (author)

  19. The role of Tc-99m sestamibi imaging in predicting clinical response to chemotherapy in lung cancer.

    Science.gov (United States)

    Dirlik, Aysegul; Burak, Zeynep; Goksel, Tuncay; Erinc, Ruya; Karakus, Haydar; Ozcan, Zehra; Veral, Ali; Ozhan, Mustafa

    2002-04-01

    Multidrug resistance (MDR) is a major problem in lung cancer. Tc-99m methoxyisobutyl isonitrile (MIBI) has been demonstrated to be a non-invasive marker to diagnose MDRI related P-glycoprotein (Pgp) and multidrug resistance-associated protein (MRP) expression in various solid tumors. The aim of this study was to evaluate the relationship between the degree of Tc-99m MIBI uptake and its retention on delayed images and the response to chemotherapy in lung cancer. Twenty-three patients (1 woman and 22 men, age range 40-67 years) with lung cancer (9 small cell and 14 non-small cell) were examined with Tc-99m MIBI imaging before chemotherapy. After i.v. administration of 740 MBq Tc-99m MIBI, planar and SPECT imaging at 30 minutes and 2 hours was performed. Tumor to normal lung uptake ratio (T/N) and percent retention were measured. Response to chemotherapy was evaluated according to follow-up CT and grouped as complete responders (CR), partial responders (PR) and non-responders (NR). Clinical follow-up and CT evaluation revealed that 12 patients had partial remission, 4 patients had complete remission and 7 patients had no-remission after chemotherapy. Statistically, there was no significant correlation between early (30 min), delayed (2 hr) T/N ratios and percent retention of Tc-99m MIBI with chemotherapeutic response of the lung cancer among the three groups (p > 0.05). Results of the current study imply that Tc-99m MIBI uptake and the retention index may not correlate with chemotherapy response in lung cancer, so that the accuracy of this method needs to be verified in a larger series with additional investigation at the molecular level.

  20. The Relationship Between Technetium-99m-Methoxyisobutyl Isonitrile Parathyroid Scintigraphy and Hormonal and Biochemical Markers in Suspicion of Primary Hyperparathyroidism

    Directory of Open Access Journals (Sweden)

    Güler Silov

    2013-04-01

    Full Text Available Objective: Technetium-99m-methoxyisobutyl isonitrile (Tc-99m MIBI has been widely used to evaluate hyperfunctioning autonomous parathyroid glands in patients with elevated intact parathyroid hormone (iPTH and/or calcium (Ca level. The aim of this study was to evaluate the relationship between Tc-99m MIBI parathyroid scintigraphy and hormonal and biochemical markers in suspicion of primary hyperparathyroidism (PHPT. Material and Methods: Dual-phase Tc-99m MIBI parathyroid scintigraphy and total serum iPTH, Ca, phosphorus (P and albumin measurements were performed in 60 patients (52 females, 8 males; mean age, 59.38±12.51 years; range, 34 to 86 years with suspicion of PHPT. Results: The iPTH median level was 160.3 pg/mL (47.8 to 782.6. Thirty-five of the patients had surgical resection of hyperfunctioning parathyroid glands. Of the 35 patients, parathyroid gland pathology was detected in 30 patients using scintigraphic examination. Tc-99m MIBI parathyroid scintigraphy was negative in 30 patients. The iPTH, Ca and P levels were significantly different between in the Tc-99m MIBI positive group and the negative group, respectively: For iPTH, 202.1 (47.8-782.6 pg/mL versus 111.6 (80.1-373 pg/mL; p0.05. Tc-99m MIBI parathyroid scintigraphy showed good correlation with iPTH level and histopathological diagnosis. Sensitivity and specificity was found 83.3% and 76.7%, respectively at the level of iPTH>147.7pg/mL. Conclusion: Tc-99m MIBI parathyroid scintigraphy is most likely to produce identification and localization of a parathyroid adenoma when both iPTH and Ca are elevated as well as decreased P levels.

  1. Biodistribution dosimetric study of radiopharmaceutical {sup 99mT}c Ixolaris in mice for melanoma diagnosis by molecular image and translational model for human beings; Estudo dosimetrico da biodistribuicao do radiofarmaco Ixolaris-{sup 99m}Tc em camundongos para diagnostico de melanoma atraves de imagem molecular e modelo translacional para humanos

    Energy Technology Data Exchange (ETDEWEB)

    Soriano, Sarah Canuto Silva

    2015-07-01

    The labeling of Ixolaris with {sup 99m}Tc was developed by Barboza et.al. (2013) aiming its use primarily in glioblastoma and after in melanoma diagnosis, a less common but very aggressive cancer and with high mortality rate. Preliminary tests on animals have proven its effectiveness of labeling but a dosimetric study to human clinical trials should be performed. This study aimed to: (1) determine the biokinetic model for the radiotracer {sup 99m}Tc-Ixolaris in mice by imaging dosimetry method; and (2) estimate the absorbed and effective dose resulting from the use of a new radiopharmaceutical for melanoma and metastases diagnosis in human beings, since a dosimetric study of new radiopharmaceuticals in animals is necessary to test them subsequently in humans and apply for registration in ANVISA. According to SPECT images, was found a latency period of 15 to 21 days for the development of lung metastasis in mice. Three C57BL6 mice, one control animal, and two animals with induced cell line B16-F10 murine melanoma were tested. The {sup 99m}Tc-Ixolaris radiopharmaceutical was administered intravenously in a caudal vein, and SPECT images were acquired 0.5 h, 1.5 h, 2.5 h, 3.5 h and 24 h post-administration for analysis and biodistribution quantification. The biokinetic model was determined and thus, obtained cumulative activity in order to estimate the absorbed dose in each organ. The mass and metabolic differences between mice and humans were considered and used to extrapolate the data acquired at different scales. Based on dose factors provided by the software MIRDOSE and Olinda (S factor), absorbed doses in irradiated target organs were calculated for the source organs, and finally the effective dose was estimated. The results indicate that for diagnostic exams conducted in human melanoma patients by administering approximately 25.7 MBq the estimated effective dose was 4.3 mSv. Comparing with effective doses obtained in other diagnostic techniques with {sup 99m

  2. Synthesis of the d,I-HM-PAO and formulation of nucleo-equipment for the obtention of {sup 99m} Tc-(d,I)-HM-PAO; Sintesis del d,I-HM-PAO y formulacion de nucleo-equipos para la obtencion de {sup 99m} Tc-(d,I)-HM-PAO

    Energy Technology Data Exchange (ETDEWEB)

    Lezama C, J.; Ferro F, G.; Alcazar A, P

    1991-09-15

    Most brain imaging radiopharmaceuticals are conventional hydrophilic compounds that are excluded from entering the normal brain by an intact blood-brain barrier (BBB). Under pathologic conditions, the barrier is disrupted and radiotracer concentrates in the leisure for positive identification. {sup 99m} Tc- hexa methyl propylene amine oxime ({sup 99} {sup m} Tc-HM-PAO) is a newer-type lipophilic agent that enter the normal brain through an intact BBB. Studies with this agent offer the promise of measuring cerebral perfusion in the normal and diseased brain. In this paper we present the synthesis and Tc-99m labelling of d,I-HM-PAO. The synthesis of the ligand was carried out by condensation of two molecular equivalents of butanedione monoxime with one molecular equivalent of 1,3 propanediamine provided a bis imine intermediate, which was reduced with sodium borohydride to get the meso and d,I diastereoisomers of HM-PAO. Separation of these was achieved by fractional crystallization. {sup 99m} Tc-(d,I)HM-PAO was obtained by stannous ion reduction of Mo-99/Tc-99m generator eluate in the presence of the ligand. Complex radiochemical purity was determined by instant thin layer chromatography and paper chromatography. Finally, we obtained {sup 99m} Tc-(d,I)HM-PAO with a high radiochemical yield, in excess of 90%. However, for subsequent clinical studies the preparation has to be done a few minutes before application because our product has a low stability. (Author)

  3. Background {sup 99m}Tc-methoxyisobutylisonitrile uptake of breast-specific gamma imaging in relation to background parenchymal enhancement in magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Hai-Jeon; Kim, Bom Sahn [Ewha Womans University, Department of Nuclear Medicine, Yangchun-Ku, Seoul (Korea, Republic of); Kim, Yemi [Ewha Womans University, Clinical Research Institute and Department of Conservative Dentistry, Seoul (Korea, Republic of); Lee, Jee Eun [Ewha Womans University, Department of Radiology, Seoul (Korea, Republic of)

    2015-01-15

    This study investigated factors that could affect background uptake of {sup 99m}Tc- methoxyisobutylisonitrile (MIBI) on normal breast by breast-specific gamma imaging (BSGI). In addition, the impact of background {sup 99m}Tc-MIBI uptake on the diagnostic performance of BSGI was further investigated. One hundred forty-five women with unilateral breast cancer who underwent BSGI, MRI, and mammography were retrospectively enrolled. Background uptake on BSGI was evaluated qualitatively and quantitatively. Patients were classified into non-dense and dense breast groups according to mammographic breast density. Background parenchymal enhancement (BPE) was rated according to BI-RADS classification. The relationship of age, menopausal status, mammographic breast density, and BPE with background {sup 99m}Tc-MIBI uptake was analyzed. Heterogeneous texture and high background uptake ratio on BSGI were significantly correlated with younger age (p < 0.001, respectively), premenopausal status (p < 0.001 and p = 0.003), dense breast (p < 0.001, respectively), and marked BPE (p < 0.001, respectively). On multivariate analysis, only BPE remained a significant factor for background MIBI uptake (p < 0.001).There was a significant reduction in positive predictive value (p = 0.024 and p = 0.002) as background MIBI uptake and BPE grade increased. BPE on MRI was the most important factor for background MIBI uptake on BSGI. High background MIBI uptake or marked BPE can diminish the diagnostic performance of BSGI. (orig.)

  4. Lyophilized kits of diamino dithiol compounds for labelling with {sup 99m}-technetium. Pharmacokinetics studies and distribution compartmental models of the related complexes; Conjuntos de reativos liofilizados de compostos diaminoditiolicos para marcacao com tecnecio-99m. Estudo farmacocinetico e elaboracao de modelos compartimentalizados dos respectivos complexos

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Elaine Bortoleti de

    1995-07-01

    The present work reflects the clinical interest for labelling diamino dithiol compounds with technetium-99m. Both chosen compounds, L,L-Ethylene dicysteine (L,L-EC) and L,L-Ethylene dicysteine diethyl esther (L,L-ECD) were obtained with relative good yield and characterized by IR and NMR. The study of labelling conditions with technetium-99m showed the influence of the type and mass of reducing agent as well as the pH on the formation of complexes with desired biological characteristics. Radiochemical purity was determined by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Lyophilised kits of L,L-EC and L,L-ECD for labelling with {sup 99m}Tc were obtained, with stability superior to 120 days, when stored under refrigeration, enabling the kits marketing. The ideal formulation of the kits as well as the use of liquid nitrogen in the freezing process, determined the lyophilization success. Distribution biological studies of the {sup 99m}Tc complexes were performed on mice by invasive method and on bigger animals by scintigraphic evaluation. Biological distribution studies of the complex {sup 99m}Tc-L,L-EC showed fast blood clearance, with the elimination of about 90% of the administered dose after 60 minutes, almost exclusively by the urinary system. The biological distribution results were adjusted to a three compartmental distribution model, as expected for a radiopharmaceutical designed to renal dynamic studies, with tubular elimination. The complex interaction with renal tubular receptors is related with structural characteristics of the compound, more specifically with the presence and location of polar groups. In comparison with {sup 99m}Tc-L,L-EC, biological studies of the complex {sup 99m}Tc -L,L-ECD showed different distribution aspects, despite some structural similarities. The presence of ethyl groups confers to the complex neutrality and lipophilicity. It cross the intact blood brain barrier and is retained in the brain

  5. Dosimetric contribution of organs of biokinetics of {sup 99m}Tc and {sup 123}I to estimate radiation doses in thyroids of children of 1 and 5 years; Contribucion dosimetrica de organos de la biocinetica del {sup 99m}Tc y {sup 123}I para estimar dosis en tiroides de ninos de 1 y 5 anos

    Energy Technology Data Exchange (ETDEWEB)

    Vasquez, M.V.; Castillo, C.E.; Abanto, D.M.; Rocha, D.; Garcia, W.H.; Marin, K., E-mail: marvva@hotmail.com [Universidad Nacional de Trujillo (UNT), Trujillo (Peru); Quispe, R. [Centro de Ciencias Aplicadas y Desarrollo Tecnologico, UNAM, Mexico (Mexico)

    2015-07-01

    The absorbed doses by thyroids during uptake studies through biokinetics of radiopharmaceuticals containing {sup 123}I (iodine) or {sup 99m}Tc (pertechnetate) are estimated. Using the MIRD scheme and the representation of Cristy-Eckerman for thyroids in children of 1 and 5 years, the objective of the study was to determine whether the dosimetric biokinetic contributions of the organs of {sup 123}I (iodide) and {sup 99m}Tc (pertechnetate) biokinetic are significant in the estimated of the absorbed dose for thyroid uptake studies.

  6. Ressonância magnética vs cintilografia com pirofosfato marcado com tecnécio-99m para a detecção de necrose miocárdica perioperatória Magnetic resonance vs technetium-99m pyrophosphate scintigraphy in the detection of perioperative myocardial necrosis

    Directory of Open Access Journals (Sweden)

    Guilherme Urpia Monte

    2008-08-01

    Full Text Available FUNDAMENTO: O infarto do miocárdio perioperatório (IMPO é uma complicação da cirurgia de revascularização miocárdica (CRM com potencial impacto prognóstico. A cintilografia miocárdica (CM com pirofosfato marcado com tecnécio-99m é utilizada no diagnóstico de IMPO, mas demonstra limitada sensibilidade para lesões subendocárdicas. A ressonância magnética cardiovascular (RMC, por sua vez, detém alta acurácia para a detecção de necrose miocárdica. OBJETIVO: Comparar a RMC e a CM para a detecção de IMPO após CRM. MÉTODOS: Foram estudados 24 pacientes portadores de doença arterial coronária crônica, com a técnica de realce tardio pela RMC e com a CM, antes e depois da CRM, analisando-se o surgimento de áreas de necrose miocárdica perioperatória (IMPO. Mensuraram-se também marcadores bioquímicos de lesão miocárdica (CKMB e troponina I, antes e depois da cirurgia. RESULTADOS: Dezenove pacientes completaram o estudo. Desses, 6 (32% apresentaram IMPO à RMC, e 4 (21% à CM (p = NS. Dos 323 segmentos do ventrículo esquerdo avaliados, 17 (5,3% exibiram necrose perioperatória à RMC, e 7 (2,2% à CM (p = 0,013. Observou-se moderada concordância entre os métodos (kappa = 0,46, havendo divergência, quanto ao diagnóstico de IMPO, em 4 (21% casos, a maioria com pequenas áreas de necrose perioperatória à RMC, não visualizadas à CM. Em todos os casos com IMPO à RMC, houve elevação significativa de CKMB e troponina I. CONCLUSÃO: Houve moderada concordância diagnóstica entre os métodos para a detecção de IMPO, mas a RMC permitiu a visualização de pequenas áreas de necrose miocárdica perioperatória, não identificadas pela CM e associadas à elevação de marcadores bioquímicos de lesão miocárdica.BACKGROUND: Perioperative myocardial infarction (POMI is a complication of coronary artery bypass grafting (CABG with a potential prognostic impact. Technetium-99m pyrophosphate myocardial scintigraphy (MS is

  7. Reduction of 99mTc-sestamibi and 99mTc-tetrofosmin uptake in MRP-expressing breast cancer cells under hypoxic conditions is independent of MRP function

    International Nuclear Information System (INIS)

    Hypoxia reduces the uptake of technetium-99m sestamibi (MIBI) in human cancer cell lines. In the current investigation, we attempted to identify the relationship between hypoxia-induced alteration of 99mTc-MIBI accumulation and expression of multi-drug resistance-associated protein (MRP) in the MCF7/WT breast cancer cell line and its subclonal cell line, MCF7/VP, which expresses high levels of MRP1. A second cationic compound, 99mTc-tetrofosmin (TF), was also examined. Cellular uptake of 99mTc-MIBI and 99mTc-TF was significantly higher in parental MCF7/WT cells than in MCF7/VP cells. Hypoxic conditions generated with a mixture of 95% N2 and 5% CO2 reduced cellular uptake of the two tracers in both parental MCF7/WT cells and MRP1-expressing MCF7/VP cells. Cell binding assay with iodine-125-labelled anti-MRP1 antibody demonstrated its specific binding to MCF7/VP cells. Hypoxia did not affect the amount of antibody bound to MCF7/VP cells. These results indicate that hypoxia-induced reduction of tracer uptake in tumour cells is a phenomenon independent of MRP function. (orig.)

  8. Technetium-99m nitrido radiopharmaceuticals with unprecedented biological properties

    Directory of Open Access Journals (Sweden)

    Adriano Duatti

    2002-09-01

    Full Text Available The chemical methods for the production of technetium-99m radiopharmaceuticals containing a terminal TcºN triple bond have been established more than a decade ago. From that time, the chemistry of nitrido Tc-99m complexes has provided a highly efficient tool for the design and preparation of novel classes of diagnostic agents, and a number of potentially useful radiopharmaceuticals have been discovered. In particular, nitrido technetium-99m tracers have been developed for heart perfusion imaging. In this short review, the chemical and biological properties of the neutral myocardial perfusion tracer bis(N-ethoxy, N-ethyl-dithiocarbamato nitrido Tc-99m (TcN-NOEt will be summarized along with the preparation and preliminary biological evaluation of the first class of monocationic nitrido technetium-99m radiopharmaceuticals exhibiting improved biodistribution properties closer to those expected for an ideal perfusion imaging agent.Os métodos químicos para produção de radiofármacos marcados com tecnécio-99m contendo a ligação tripla terminal TcºN foram estabelecidos há mais de uma década. Desde esta época, a química dos complexos nitridos marcados com 99mTc tem sido uma ferramenta altamente eficiente para o desenho e preparo de novas classes de agentes para diagnóstico e, foi descoberto um número de radiofarmacos potencialmente úteis. Nesta pequena revisão, as propriedades biológicas e químicas do traçador para perfusão miocárdica neutra, o bis(N-etoxi, N-etil-ditiocarbamato nitrido 99mTc (TcN-NOEt, serão resumidas junto com o preparo e avaliação biológica preliminar da primeira classe de radiofármacos nitrido monocatiônico marcado com tecnécio-99m que exibe melhores propriedades em relação à biodistribuição, mais próximas daquelas esperadas para um agente perfusor ideal para imagens.

  9. Padronização do método para cálculo da captação renal absoluta do99mTc-DMSA em cria Standardization of a method to calculate absolute renal uptake of 99mTc-DMSA in children

    OpenAIRE

    Carla Rachel Ono; Marcelo Tatit Sapienza; Beatriz Marcondes Machado; Márcia Melo Campos Pahl; Waldyr de Paula Liberato Jr.; Miriam Roseli Yoshie Okamoto; Alexandre Teles Garcez; Tomoco Watanabe; Paulo Luiz Aguirre Costa; Carlos Alberto Buchpiguel

    2006-01-01

    OBJETIVO: O trabalho teve por objetivo padronizar o método e estabelecer valores normais da captação renal absoluta do99mTc-DMSA em crianças. MATERIAIS E MÉTODOS: Vinte e duas crianças (idade de 7 meses a 10 anos; média de 4,5 anos) sem doença renal prévia foram submetidas a cintilografia renal estática com 99mTc-DMSA. Dezoito apresentavam ultra-sonografia, uretrocistografia miccional, "clearance" de creatinina e padrão visual da cintilografia renal estática normais. Quatro crianças foram exc...

  10. Preparation of the radiopharmaceutical {sup 99m} Tc-HYNIC-cyclo-Lys-D-Phe-RGD for In vivo image of integrines; Preparacion del radiofarmaco {sup 99m} Tc-HYNIC-ciclo-Lys-D-Phe-RGD para imagen In vivo de integrinas

    Energy Technology Data Exchange (ETDEWEB)

    Hernandez H, E. [ININ, 52750 La Marquesa, Estado de Mexico (Mexico)

    2007-07-01

    The diagnostic of some pathological processes by means of images constitutes one of the used methods in the determination of the origin, condition and/or evolution of one illness. The use of contrast agents in conjunction with other techniques help to the obtaining and visualization of complex systems, among these we can find to those radiopharmaceuticals used in nuclear medicine to visualize diverse organs and corporal systems. At the moment it is sought to develop a radiopharmaceutical of third generation that can be used for image In vivo of integrines with the purpose of detecting angio genesis processes, that which would allow to diagnose in way it specifies a wide range of primary tumors and their metastasis. Presently work it developed the radiopharmaceutical {sup 99m}Tc-HYNIC-cycle-Lys-D-Phe-RGD, likewise the good conditions were determined for the formation of this complex. The HYNIC was employee as chelating agent, using as co ligands EDDA and Tricine for to complete the sphere of coordination of the {sup 99m}Tc. The conjugated HYNIC-RGD was synthesized, purified, characterized and radiolabelled In situ with {sup 99m}Tc using High pressure liquid chromatography as analysis method in Reverse Phase (RP-HPLC). By this way it was developed the lyophilized formulation for its instantaneous labelled to which were carried out quality control tests. The one conjugated was obtained free of impurities, showing stability at same as their complex formed with {sup 99m}Tc. The analysis method was validated turning out to be necessary, exact, lineal and specific for the quantification of the analyte of interest. The lyophilized formulation showed a radiochemical purity bigger than 95%, besides being sterile and free of pyrogens. The biodistribution tests in athymic mice with induced tumors showed that the radiopharmaceutical was united mainly to the tumor and that this it was excreted mainly for renal via. (Author)

  11. The effect of Tc99m Sestamibi scans during acute chest pain on clinical management

    International Nuclear Information System (INIS)

    Full text: The aim of this study is to assess whether the increased sensitivity and specificity of Tc99m sestamibi scans, during acute chest pain, will lead to alteration in clinical management and potential cost saving in an Australian population. Consecutive patients who presented with acute chest pain were injected 800 MBq of Tc99m sestamibi during pain (Hot MlBI) and SPECT imaging performed 1-6 hours later. The population was those only with a 'intermediate risk' of myocardial ischaemia The patients included in patients, those in the Emergency Department, and those with a previous history of cardiac disease. 25% of patients required a second, pain free study the following day to differentiate acute ischaemia from prior infarction. A question sheet was filled out by the requesting physician prior to the study indicating the likelihood of cardiac disease and the proposed management if no 'Hot MIBI' scan was available. The treatment that the patient subsequently received was ascertained from the patient's medical record. Of the 28 patients, a prediction whether to or not to proceed to coronary angiography was made in 13 patients prior to the MIBI study being performed. Of the 13, 5 would have had coronary angiography performed. and in all 5, the decision to proceed to coronary angiography was averted by the 'Hot MIBI'. Of note, 3 patients were admitted purely because of an abnormal 'Hot MIBI'. The 'Hot MIBI' was able to reduce coronary care admissions by 83% reduce all admissions by 17%, and avert coronary angiography in 38% of patients. In this intermediate risk category patient, this translates to not only admissions saved but potential cost saving

  12. Optimization of preparation, labelling and quality control procedures of {sup 99m}Tc-glucarate for the diagnosis of acute myocardial infarction; Otimizacao dos procedimentos de preparacao, marcacao e controle de qualidade do glucarato-{sup 99m}Tc para diagnostico do infarto agudo do miocardio

    Energy Technology Data Exchange (ETDEWEB)

    Camargo, Ana Claudia

    2007-07-01

    Cardiovascular diseases account for the main causes of morbidity and mortality among the adult population. Despite the advances of preventive medicine, acute myocardial infarction continues to be frequent and the difficulty of the differential diagnosis is an additional cause of fatal events. Its diagnosis is based on the triad: typical chest pain, alterations in the electrocardiogram and elevation of serum cardiac enzymes. However, many patients evolve without the complete syndrome. Therefore the development of non-invasive assays for the early detection of damaged myocardium is of extreme importance. {sup 99m}Tc-glucarate is a radiopharmaceutical of great interest for application in Nuclear Medicine, because it is referred as a potential marker of necrotic tissues in the early phase of an acute coronary event. This presentation describes the optimization of preparation, lyophilization and labelling as well as quality control procedures of {sup 99m}Tc-glucarate, based on former studies published in the literature. Radiochemical purity was evaluated by the ascending paper chromatography technique. Labelling efficiency as a function respectively of time of reaction, volume and activity of {sup 99m}TcO4- was consistently higher than 97%. The lyophilized kit was stable during 12 months of storage. Biodistribution in healthy Swiss mice showed rapid blood clearance, renal excretion and low uptake by organs adjacent to the heart. Scintigraphic studies of injected Wistar rats confirmed the results of the biodistribution obtained by direct organ counting. Scintigraphies of infarcted female rats proved the sensitivity of the method. (author)

  13. Technetium-99m sestamibi uptake in human breast carcinoma cell lines displaying glutathione-associated drug-resistance

    Energy Technology Data Exchange (ETDEWEB)

    Kabasakal, L. [Dept. of Nuclear Medicine and Biochemistry, Medical College of Wisconsin, Milwaukee, WI (United States); Oezker, K. [Dept. of Nuclear Medicine and Biochemistry, Medical College of Wisconsin, Milwaukee, WI (United States); Hayward, M. [Dept. of Nuclear Medicine and Biochemistry, Medical College of Wisconsin, Milwaukee, WI (United States); Akansel, G. [Dept. of Nuclear Medicine and Biochemistry, Medical College of Wisconsin, Milwaukee, WI (United States); Griffith, O. [Dept. of Nuclear Medicine and Biochemistry, Medical College of Wisconsin, Milwaukee, WI (United States); Isitman, A.T. [Dept. of Nuclear Medicine and Biochemistry, Medical College of Wisconsin, Milwaukee, WI (United States); Hellman, R. [Dept. of Nuclear Medicine and Biochemistry, Medical College of Wisconsin, Milwaukee, WI (United States); Collier, D. [Dept. of Nuclear Medicine and Biochemistry, Medical College of Wisconsin, Milwaukee, WI (United States)

    1996-05-01

    An in vitro study was designed to evaluate the uptake of sestamibi (MIBI) in P-glycoprotein (Pgp) and glutathione-associated (GSH) multidrug-resistant (MDR) cell lines. MIBI uptake was studied in various human breast carcinoma cell lines, i.e. in wild-type (MCF7/wt) cells, in adriamycin-resistant (MCF7/adr) cells which express Pgp and in melphalan-resistant (MCF7/mph) cells with increased levels of GSH. The effects of buthiomine sulphoximine (BSO) and verapamil on MIBI uptake were also studied in the MCF7/mph and MCF7/adr cells respectively. The cells were incubated for 1 h with a dose of 0.1 MBq thallium-201 and technetium-99m MIBI. Both BIBI and {sup 201}Tl uptakes were higher for MCF7/mph cells than for the other cells studied. The mean MIBI uptake in MCF7/adr cells was significantly lower than that in MCF7/wt cells (1.9%{+-}0.5% vs 3.1%.0.6%; P<0.01). Verapamil treatment increased the MIBI uptake in MCF7/adr cells (to 2.6%.0.3%; P<0.05). Treatment of MCF7/mph cells with BSO resulted in a significant reduction in GSH content (from 243.2{+-}81.1 nmol/mg protein to 17.6{+-}4.4 nmol/mg protein; P<0.001). However, MIBI uptake in BSO-treated and untreated MCF7/mph cells was similar (4.43%{+-}0.5% and 5.93%{+-}1.7%, respectively; P>0.1). This study suggests that the uptake of MIBI is not diminished by glutathione-associated drug resistance and that MIBI uptake in a tumour sample does not necessarly indicate that a cancer is sensitive to drugs. (orig.)

  14. Study of the viability of technetium-{sup 99m} labeling of whole antimyosin antibody and its fragment: development of radiopharmaceutical for cardiac survey; Estudo da viabilidade da marcacao com tecnecio-99m do anticorpo antimiosina integro e seu fragmento: desenvolvimento de radiofarmaco para avaliacao cardiaca

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Guilherme Luiz de Castro

    2007-07-01

    In the acute myocardium infarction, the myocytes cell membrane loses its integrity, allowing the influx of extracellular macromolecules such as circulating antibody into the damaged cell. The use of the specific antibodies against cardiac myosin labeled with {sup 99m}Tc allows to determine the localization and extension of myocardial infarction. The purpose of this work was to study the viability of labeling of the antimyosin monoclonal antibody and its fragment F(ab')2 with {sup 99m}Tc. Because of the high cost of antimyosin antibody, others antibodies were used to optimize the methodology and the best condition was used for antimyosin antibody. The intact antibody was cleaved by pepsin to produce F(ab'){sub 2} fragment. The F(ab'){sub 2} and the intact antibody were reduced by treatment with Dithiothreitol (DTT) and 2-Mercaptoethanol (2-ME) and labeled with {sup 99m}Tc by direct method. Different concentrations of reductant, mixing conditions and incubation times were studied. In the standard condition, incubation at molar ratio 1:1000 (antibody:reducing agent) at room temperature for 30 minutes with continuous rotation (850 rpm), 13.28 - SH groups were formed per molecule. It was studied the influence of p H, of the concentration of stannous chloride (Sn{sup 2+}) and incubation time in the labeling condition. The better radiochemical yield (90.06 +- 1.53%) was obtained using 2.5 {mu}g of Sn{sup 2+} in p H 4.5 for 60 minutes. The labeling of the fragment F(ab'){sub 2} did not present satisfactory results because of the low yield of the digestion. After purification by PD-10, the biodistribution study was performed and showed that the intact antimyosin antibody labeled with {sup 99m}Tc presented fast kinetic compatible with the biodistribution of an intact antibody labeled with {sup 99m}Tc. Scintigraphy image of the animal with myocardial infarction was obtained and compared with the image of a normal animal. The studies allow to conclude that

  15. Dimer of the peptide cycle (Ar-Gly-Asp-D-Phe-Lys) radiolabeled with {sup 99m}Tc for the integrin s over-expression image: formulation, biokinetics and dosimetry; Dimero del peptido ciclo(Arg-Gly-Asp-D-Phe-Lys) radiomarcado con {sup 99m}Tc para la imagen de sobre-expresion de integrinas: formulacion, biocinetica y dosimetria

    Energy Technology Data Exchange (ETDEWEB)

    Ortiz A, Z.

    2013-07-01

    In breast cancer, α(v)β(3) and/or α(v)β(5) integrin s are over-expressed in both endothelial and tumour cells. Radiolabeled peptides based on the RGD (Arg-Gly-Asp) sequence are radiopharmaceuticals with high affinity and selectivity for those integrin s. The RGD-dimer peptide (E-[c(RGDfK)]{sub 2}) radiolabeled with {sup 99m}Tc has been reported as a radiopharmaceutical with 10-fold higher affinity for the α(v)β(3) integrin as compared to the RGD-monomer. EDDA (Ethylenediamine-N,N-diacetic acid) is a hydrophilic molecule that may favours renal excretion when used as coligand in the {sup 99m}Tc labelling of HYNIC-peptides and can easily be formulated in a lyophilized kit. Aim: Establish a biokinetic model for {sup 99m}Tc-EDDA/HYNIC-E-[c(RGDfK)]{sub 2} prepared from lyophilized kits and evaluate the dosimetry as breast cancer imaging agent. Methods: {sup 99m}Tc labelling was performed by addition of sodium pertechnetate solution and 0.2 M phosphate buffer ph 7.0 to a lyophilized formulation containing E-[c(RGDfK)]{sub 2}, EDDA, tricine, mannitol and stannous chloride. Radiochemical purity was evaluated by reversed phase HPLC and ITLC-SG analyses. Stability studies in human serum were carried out by size-exclusion HPLC. In-vitro cell uptake was tested using breast cancer cells (MCF7, T47D and MDA-MB-231) with blocked and non-blocked receptors. Biodistribution and tumour uptake were determined in MCF7 tumour-bearing nude mice with blocked and non-blocked receptors, and images were obtained using a micro-SPECT/CT. Whole-body images from seven healthy women were acquired at 1, 3, 6 and 24 h after {sup 99m}Tc-EDDA/HYNIC-E-[c(RGDfK)]{sub 2} administration obtained with radiochemical purities of >94 %. Regions of interest (ROIs) were drawn around source organs on each time frame. Each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate {sup 99m}Tc-EDDA/HYNIC-E-[c(RGDfK)]{sub 2} time-activity curves in each

  16. Cyclotron Production of Technetium-99m

    Science.gov (United States)

    Gagnon, Katherine M.

    Technetium-99m (99mTc) has emerged as the most widely used radionuclide in medicine and is currently obtained from a 99Mo/ 99mTc generator system. At present, there are only a handful of ageing reactors worldwide capable of producing large quantities of the parent isotope, 99Mo, and owing to the ever growing shutdown periods for maintenance and repair of these ageing reactors, the reliable supply 99mTc has been compromised in recent years. With an interest in alternative strategies for producing this key medical isotope, this thesis focuses on several technical challenges related to the direct cyclotron production of 99mTc via the 100Mo(p,2n)99mTc reaction. In addition to evaluating the 100Mo(p,2n)99mTc and 100Mo(p,x)99Mo reactions, this work presented the first experimental evaluation of the 100Mo(p,2n) 99gTc excitation function in the range of 8-18 MeV. Thick target calculations suggested that large quantities of cyclotron-produced 99mTc may be possible. For example, a 6 hr irradiation at 500 μA with an energy window of 18→10 MeV is expected to yield 1.15 TBq of 99mTc. The level of coproduced 99gTc contaminant was found to be on par with the current 99Mo/99mTc generator standard eluted with a 24 hr frequency. Highly enriched 100Mo was required as the target material for 99mTc production and a process for recycling of this expensive material is presented. An 87% recovery yield is reported, including metallic target preparation, irradiation, 99mTc extraction, molybdate isolation, and finally hydrogen reduction to the metal. Further improvements are expected with additional optimization experiments. A method for forming structurally stable metallic molybdenum targets has also been developed. These targets are capable of withstanding more than a kilowatt of beam power and the reliable production and extraction of Curie quantities of 99mTc has been demonstrated. With the end-goal of using the cyclotron-produced 99mTc clinically, the quality of the cyclotron

  17. Tl-201 and Tc-99m-Sestamibi SPECT for brain tumor detection: Comparison using MRI coregistration

    Energy Technology Data Exchange (ETDEWEB)

    Darcourt, J.; Itti, L.; Chang, L. [UCLA Medical Center, Torrance, CA (United States)] [and others

    1994-05-01

    Tl-201 (Tl) brain SPECT has been validated for the differential diagnosis of high versus low grade gliomas and recurrence versus radiation necrosis. We compared this technique to Tc-99m-Sestamibi (MIBI) SPECT in 9 patients (pts) with brain tumors using MRI coregistration. Pts were injected with 4 mCi of Tl and brain SPECT was performed using a dedicated brain system. This was immediately following by an injection of 20 mCi of MIBI and a brain SPECT using the same camera and with the pt in the same position. Four pts were studied for the diagnosis of radiation necrosis vs. tumor recurrence (2 had biopsy proven recurrence); 5 pts were studied for primary tumor evaluation: 2 meningiomas, 1 oligodendroglioma, 1 low-grade astrocytoma, 1 cysticercosis. Coregistration was performed for every pt by 3D surface fitting of the inner skull MIBI contour to the MRI brain surface extracted automatically. ROIs were drawn on the MRI and applied to the coregistered MIBI and Tl images for tumor to non-tumor ratios T/NT calculations. There was a tight correlation between MIBI and Tl T/NT (r-0.96) and a 1.5 threshold separated radiation necrosis from recurrence and low from high grade primary tumors. Therefore, the data already available on Tl brain tumor imaging can be used with MIBI SPECT with the advantage of a better image quality (2.5 to 4 times more counts).

  18. Usefulness of 99mTc-Sestamibi whole body scan in the follow-up of differentiated thyroid cancer

    International Nuclear Information System (INIS)

    Although radioiodine is and had been the more common used radioisotope in the follow-up of differentiated thyroid cancer (DTC), other radioisotopes, such as 99mTc sestamibi (MIBI), had been incorporated as auxiliary methods. Our objective was to assess the diagnostic usefulness of MIBI in the long term follow-up of DTC. Thirty-two patients (26 (81%) female), mean age 45 ± 12.6 years, 2 with follicular carcinoma, and 30 with papillary carcinoma, were prospectively studied with MIBI. Mean time of follow-up from thyroidectomy to MIBI was 4.6 ± 4.9 years. (range, 7 months to 22 years). Sensitivity (Sens), specificity (Spec), positive predictive value (PPV), negative predictive value (NPV), and accuracy (A) was evaluated. We assessed agreement for MIBI and 131I, by means of percent agreement and Cohen's kappa (k) statistic. The gold standard method to indicate persistent disease was serum thyroglobulin (Tg) measurement by ICMA, with cut-off level of = 2 ng/ml 'off T4', and > 0.2 ng/ml 'on T4'. In 3 patients with positive anti-Tg antibodies, 131I scan and other auxiliary methods (Ultrasound, CT scan) were considered as gold standard. For MIBI the values of Sens, Spec, PPV, NPP, and A were 29% (26-32), 93% (89-96), 83% (74-91), 53% (51-55), and 59% (57-61), respectively. For 131I were 31% (27-34), 100% (96-100), 100% (90-100), 56% (53-58), and 63% (61-65), respectively. The percent agreement between both radiopharmaceuticals was 76.6%, and k was 0.2 (-0.19 -0.63). Similar statistical indicators for MIBI and 131I warrant the use of the former as alternative method. At the same time, a fair agreement between both radiopharmaceuticals demonstrates the additive information offered by MIBI in the follow-up of DTC. (author)

  19. Dosimetric evaluation in organs of the Tc{sup 99m}, I{sup 123} bio-kinetics to estimate dose in thyroid children 1 and 5 years; Evaluacion dosimetrica en organos de la biocinetica del Tc{sup 99m}, I{sup 123} para estimar dosis en tiroides ninos 1 y 5 anos

    Energy Technology Data Exchange (ETDEWEB)

    Vasquez, A. M.; Quispe, R.; Vasquez, D. J.; Rocha, M. D.; Morales, N. R.; Marin, R. K. [Universidad Nacional de Trujillo, Av. Juan Pablo II s/n, Ciudad Universitaria, Trujillo (Peru); Zelada, A. L., E-mail: marvva@hotmail.com [Universidad Cesar Vallejo, Grupo de Fisica Medica, Av. Larco s/n, Trujillo (Peru)

    2012-10-15

    Using the formalism MIRD and the representation of Cristy-Eckerman for the thyroid in children of 1 and 5 years, is demonstrated that the dosimetric contribution of the organs of I{sup 123} (iodure) bio-kinetics is not significant in the dose estimate. The total dose absorbed by the gland is its auto dose. The dosimetric contribution of the organs source of the Tc{sup 99m} (pertechnetate) bio-kinetics in the gland is significant in the dose estimate like to be ignored. The reported results for the iodure are not significantly different to the found for the Marinelli scheme (auto-dose) for thyroid represented by a sphere of 1,78 and 3,45 grams. (Author)

  20. Predictive value of technetium-99m sestamibi in patients with multiple myeloma and potential role in the follow-up

    Energy Technology Data Exchange (ETDEWEB)

    Pace, L.; Del Vecchio, S.; Salvatore, M. [Cattedra di Medicina Nucleare, Dipt. di Scienze Biomorfologiche e Funzionali, Univ. Federico II, Napoli (Italy); Centro C.N.R. per la Medicina Nucleare, Napoli (Italy); Catalano, L.; De Renzo, A.; Califano, C.; Rotoli, B. [Cattedra di Ematologia, Dipt. di Medicina Clinica e Sperimentale, Univ. Federico II, Napoli (Italy); Di Gennaro, F.; Sica, G.; Tedesco, N.; Borrelli, G. [Cattedra di Medicina Nucleare, Dipt. di Scienze Biomorfologiche e Funzionali, Univ. Federico II, Napoli (Italy)

    2001-03-01

    Technetium-99m 2-methoxyisobutylisonitrile ({sup 99m}Tc-MIBI or setamibi) has recently been proposed for use in the evaluation of multiple myeloma (MM). The aims of this study were to investigate its potential predictive value in patients with MM and its possible role in the follow-up. Thirty patients with MM who had undergone two {sup 99m}Tc-MIBI scintigraphic studies at least 2 months apart constituted the study group; 22 of them received chemotherapy in the interval between the two scans. The scans were classified as showing pattern N when only physiological uptake was present, pattern D when diffuse bone marrow uptake was observed, pattern F when areas of focal uptake of the tracer were evident, and pattern F+D when both D and F patterns were observed. Comparative {sup 99m}Tc-MIBI scintigraphy was considered indicative of disease progression when there was a worsening of the pattern (i.e. from N to D, or from N or D to F or to F+D) or an increase in the pattern D semiquantitative score. It was considered indicative of disease improvement when the opposite trend was observed; otherwise, it was considered to document a stable condition. A significant association was observed between the baseline scintigraphic pattern and clinical status at follow-up in the group of patients evaluated after chemotherapy ({chi}{sup 2}=16.7, P<0.05). A negative baseline {sup 99m}Tc-MIBI scintigram showed a high predictive accuracy (100%) for remission, while the presence of pattern F or F+D was often associated with a less favourable outcome. A multivariate analysis showed that {sup 99m}Tc-MIBI uptake pattern has an added value in relation to known prognostic variables such as C-reactive protein. {sup 99m}Tc-MIBI scintigraphy patterns at follow-up were significantly associated with the clinical status evaluated after chemotherapy ({chi}{sup 2}=32.6, P<0.0001). Considering pattern N as indicating remission, pattern D stable condition, and pattern F or F+D progressive disease, a high

  1. Labeling of creatinine with technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Yurt Lambrecht, F. [Ege Univ., Bornova, Izmir (Turkey). Dept. of Nuclear Applications, Inst. of Nuclear Sciences; Durkan, K. [Dokuz Eylul Univ., Buca, Izmir (Turkey). Chemistry Technicianship Program, Izmir Vocational School; Soylu, A. [Dokuz Eylul Univ., Narlidere, Izmir (Turkey). Dept. of Pediatrics, Medical Faculty

    2004-07-01

    Creatinine is a clinically important index of renal glomerular filtration rate. Urine creatinine levels can be used as a screening test to evaluate kidney function or can be part of the creatinine clearance test. In case of kidney dysfunction or muscle disorders the creatinine concentration in serum/plasma may rise to a higher value than in healthy body. Technetium- 99m has been used in nuclear medicine and in biomedical research to label molecular and cellular structures employed as radiotracers. {sup 99m}Tc is utilized to label molecules and cells, used as radiopharmaceuticals, and also to label biological species. It presents many desirable characteristics. SnCl{sub 2} method is frequently used as a reducing agent in the {sup 99m}Tc- labeling process. Creatinine metabolism might be investigated by using labeled {sup 99m}Tc- creatinine in healthy or uremic rats. (orig.)

  2. Novel Tc-99m radiotracers for brain imaging

    Directory of Open Access Journals (Sweden)

    Alessandra Boschi

    2007-09-01

    Full Text Available A novel class of Tc-99m complexes able to cross the blood brain barrier has been investigated and described here. These compounds are formed by reacting the bis-substituted nitrido precusors [99mTc(N(PS2] (PS = phosphino-thiol ligand with triethylborane (BEt3 under strictly anhydrous conditions and using non-aqueous solvents. The molecular structure of these tracers was not fully established, but preliminary experimental evidence suggests that they result from the interaction of the Lewis base [99mTc(N(PS2] with the Lewis acid BEt3, which leads to the formation of the novel -B-Nº Tc- adduct. After purification and recovery in a physiological solution, the new borane-nitrido Tc-99m derivatives were injected in rats for evaluating their in vivo biological behavior. Results showed a significant accumulation in brain tissue, thus indicating that these complexes are capable of penetrating the intact blood brain barrier. Uptake in the central nervous system was confirmed by imaging the distribution of activity on the integrated living animal using a YAP(SSPECT small animal scanner.Uma nova classe de complexos de Tc-99m capazes de atravessar a barreira hemato-encefálica foi investigada e descrita neste trabalho. Estes compostos são formados reagindo os precursores bissubstituídos do nitrido [99mTc(N(PS2] (PS = phosphino-tiol ligante com trietillborano (BEt3 sob restritas condições anidras e usando solventes não aquosos. A estrutura molecular desses traçadores não foi totalmente estabelecida, mas evidências experimentais preliminares sugerem que eles resultam da interação da base de Lewis [99mTc(N(PS2] com o ácido de Lewis BEt3, levando a formação do novo aducto -B-Nº Tc-. Após purificação e recuperação em uma solução fisiológica, os novos derivados borano-nitrido-Tc-99m foram injetados em ratos para avaliação de seu comportamento biológico in vivo. Os resultados mostraram uma acumulação significativa no tecido cerebral

  3. Under used technetium-99m generators

    International Nuclear Information System (INIS)

    Health care reform truly has become a global issue and it will undoubtedly have a dramatic impact on the future of nuclear medicine business in particular. A bigger concern within the nuclear medicine community is its competitiveness with other modalities and cost effectiveness.Technetium-99m and its generators are playing key role for the majority of diagnostic scans performed in the world today. Availability of ''9''9''mTc can be increased if it is separated from ''9''9Mo after much shorter growth times. After proper planning with the extra ''9''9''mTc, a significant number of scans can be performed or we would be able to order approximately 30% low activity ''9''9Tc generators to fulfill our requirements

  4. P-gp and MRP1 Expression in Parathyroid Tumors Related to Histology, Weight and Tc-99m-Sestamibi Imaging Results

    NARCIS (Netherlands)

    Jorna, F. H.; Hollema, H.; Hendrikse, H. N.; Bart, J.; Brouwers, A. H.; Plukker, J. T. M.

    2009-01-01

    Objective: P-glycoprotein (P-gp) and multidrug resistance-associated protein (MRP) are membrane efflux pumps that may have a role in the kinetics of Tc-99m-sestamibi (MIBI) in parathyroid tumors. P-gp and MRP1 expression in parathyroid tumors was studied and related to histology, weight and pre- and

  5. Noninvasive diagnosis of coronary artery stenosis in women with limited exercise capacity: comparison of dobutamine stress echocardiography and 99mTc sestamibi single-photon emission CT

    NARCIS (Netherlands)

    A. Elhendy (Abdou); J.J. Bax (Jeroen); P.R. Nierop; M.L. Geleijnse (Marcel); M.M. Ibrahim; J.R.T.C. Roelandt (Jos); R.T. van Domburg (Ron)

    1998-01-01

    textabstractOBJECTIVES: To compare the accuracy of dobutamine stress echocardiography (DSE) and simultaneous 99mTc sestamibi (MIBI) single-photon emission CT (SPECT) imaging for the diagnosis of coronary artery stenosis in women. PATIENTS: Seventy women with limited exe

  6. Practical aspects of the preparation of 99mTc-labeled radiopharmaceuticals using cold kit

    International Nuclear Information System (INIS)

    This paper describes notes for preparation of radiopharmaceuticals (RP) based on the legal standard, package inserts and academic papers. The nuclide 99mTc is the most popular one among RP and labels compounds by ligand-replacement or complex-formation. Radiochemical purity of RP, particularly important for the diagnostic specificity, is practically examined by chromatography (paper, thin-layer, high-performance liquid, electrophoresis, etc). The chromatographic radioactivity is detected by autoradiography, NaI(Tl) scanner or cut-and-count procedure. The sterility and milking interval are important for preparation. Finally, 15 RP specifications are summarized: 99mTc-labeled ECD, HMPAO, MIBI, tetrofosmin, HSA, pyrophosphate, MDP, HMDP, MAA, Sn colloid, phytate, MAG3, DTPA and DMSA (2 preparations). (N.I.)

  7. Comparison of SPECT/CT and planar MIBI in terms of operating time and cost in the surgical management of primary hyperparathyroidism.

    Science.gov (United States)

    Setabutr, Dhave; Vakharia, Kavita; Nogan, Stephen J; Kamel, George N; Allen, Thomas; Saunders, Brian D; Goldenberg, David

    2015-01-01

    We conducted a retrospective study to compare operating times and costs in patients who underwent guided parathyroidectomies with either (1) technetium-99m ((99m)Tc) multiplex ion-beam imaging (MIBI) parathyroid scintigraphy with single-photon emission computed tomography/computed tomography (SPECT/CT) fusion images or (2) sestamibi dual-phase (99m)Tc MIBI planar parathyroid scintigraphy alone preoperatively. Our study population was made up of the first 24 patients at our facility who had undergone SPECT/CT parathyroid imaging with technetium-99m ((99m)Tc) MIBI and a group of 24 patients who had undergone MIBI planar imaging alone. Patient demographics, preoperative laboratory test results, operating times, and hospital charges were analyzed. We found that less operating time was required for the planar MIBI group than in the SPECT/CT group (mean: 135 vs. 158 min), although the difference was not statistically significant. Likewise, the total cost of treatment was lower in the planar MIBI group (mean: $10,035 vs. $11,592); the difference was statistically significant by one measure (p × 0.02, Wilcoxon rank sum test) but not by another (p × 0.06, Student t test). Although SPECT/CT is efficient for patients with small and difficult-to-localize adenomas, it has yet to demonstrate greater efficacy or cost-effectiveness than planar MIBI for routine parathyroidectomy in patients with primary hyperparathyroidism when an easily identifiable parathyroid adenoma is localized. PMID:26535821

  8. Imaging recognition of inhibition of multidrug resistance in human breast cancer xenografts using {sup 99m}Tc-labeled sestamibi and tetrofosmin

    Energy Technology Data Exchange (ETDEWEB)

    Liu Zhonglin [Department of Radiology University of Arizona, Tucson, AZ 85724-5067 (United States)]. E-mail: zliu@radiology.arizona.edu; Stevenson, Gail D. [Department of Radiology University of Arizona, Tucson, AZ 85724-5067 (United States); Barrett, Harrison H. [Department of Radiology University of Arizona, Tucson, AZ 85724-5067 (United States); Furenlid, Lars R. [Department of Radiology University of Arizona, Tucson, AZ 85724-5067 (United States); Wilson, Donald W. [Department of Radiology University of Arizona, Tucson, AZ 85724-5067 (United States); Kastis, George A. [Department of Radiology University of Arizona, Tucson, AZ 85724-5067 (United States); Bettan, Michael [Department of Radiology University of Arizona, Tucson, AZ 85724-5067 (United States); Woolfenden, James M. [Department of Radiology University of Arizona, Tucson, AZ 85724-5067 (United States)

    2005-08-01

    Background: {sup 99m}Tc-sestamibi (MIBI) and {sup 99m}Tc-tetrofosmin (TF) are avid transport substrates recognized by the multidrug resistance (MDR) P-glycoprotein (Pgp). This study was designed to compare the properties of MIBI and TF in assessing the inhibition of Pgp by PSC833 in severe combined immunodeficient mice bearing MCF7 human breast tumors using SPECT imaging. Methods: Animals with drug-sensitive (MCF/WT) and drug-resistant (MCF7/AdrR) tumors were treated by PSC833 and by carrier vehicle 1 h before imaging, respectively. Dynamic images were acquired for 30 min after intravenous injection of MIBI/TF using a SPECT system, FastSPECT. The biodistribution of MIBI and TF was determined at the end of the imaging session. Results: MCF7/WT in the absence and presence of PSC833 could be visualized by MIBI and TF imaging within 5 min and remained detectable for 30 min postinjection. MCF7/AdrR could be visualized only 2-5 min without PSC833 treatment but could be detected for 30 min with PSC833, very similar to MCF7/WT. MCF7/AdrR without PSC833 showed significantly greater radioactive washout than MCF7/WT and MCF7/AdrR with PSC833 treatment. PSC833 increased the accumulation (%ID/g) in MCF7/AdrR 3.0-fold (1.62{+-}0.15 vs. 0.55{+-}0.05, P<.05) for TF and 1.9-fold (1.21{+-}0.04 vs. 0.64{+-}0.05, P<.05) for MIBI but did not affect MCF7/WT. Conclusions: The feasibility of MIBI and TF for assessment of MDR expression and inhibition was demonstrated in mice through FastSPECT imaging. The results indicate that TF may be at least comparable with MIBI in recognizing Pgp expression and modulation.

  9. Imaging prostatitis with Tc-99m ciprofloxacin

    International Nuclear Information System (INIS)

    Aim: Tc-99m ciprofloxacin (Infecton) imaging was developed to discriminate infection from inflammation. It claims to show an area of viable, proliferating bacterial population that is actively using DNA gyrase, which the ciprofloxacin inhibits. The application of this imaging has been reported in patients with infected arthroplasty, soft tissue infection, pelvic inflammatory diseases, etc. Clinical evaluation with conventional urological laboratory tests often provides insufficient data in differentiating chronic bacterial prostatitis (CBP) from pelvic pain syndrome, which causes inappropriate or inadequate antibiotic therapy without definite evidence of presence or absence of bacterial infection. Therefore, we investigated the diagnostic value of the Tc-99m ciprofloxacin imaging for CBP by comparing the results of the imaging with those from the conventional tests. Materials and Methods: We custom-formulated a Tc-99m ciprofloxacin kit. We used stannous tartrate for a reducing agent in making the kit. SPECT images of the region of interest were obtained at 3 hours after injection of the radiopharmaceutical. For positive control, we imaged 2 patients: acute prostatitis and urethritis. Then the actual study included 20 subjects. We excluded from the study the patients who had received antibiotic therapy within 6 weeks. The conventional tests included CBC, urinalysis, 4-glass tests, microscopic examination of expressed prostatic secretion, bacterial culture, or PCR for chlamydia trachomatis. Kappa statistics were obtained for comparison of the two tests. Results: By conventional tests, there were 10 subjects with CBP and 10 normal subjects. The typical pattern in the Tc-99m ciprofloxacin imaging indicating CBP was the increased uptake around the prostatic urethra. Five of the 10 subjects with CBP diagnosed by conventional tests showed the increased uptake to be positive cases in the Tc-99m ciprofloxacin imaging. Of the 10 subjects diagnosed as normal by the

  10. Gentc99m, computational system for the technetium-99m generator

    International Nuclear Information System (INIS)

    The technetium-99m generator is one of the main products of the PPR, as the continuity of the technetium-99m generator production is important for supporting the development of nuclear medicine. GENTC99M has been made for computational for the technetium-99m generator and includes data processing, documentation and information GENTC99M is also very useful in quality control application especially for the determinations of yield and radionuclidic impurities which consume much time. microsoft visual basic for MS-DOS and visual basic for windows have been used for making GENTC99M. Microsoft visual basic has several features that make it an ideal development language for both MS-DOS and Microsoft Windows. These features not only increase productivity, they also provide all the tools and hooks needed to develop some very sophisticated applications. for a production centre like PPR, GENTC99M is very useful to support the data processing, documentation and information system of the technetium-99m generator and it can also be modified for other products

  11. Biodistribution studies of 99mTc-labeled myoblasts in a murine model of muscular dystrophy

    International Nuclear Information System (INIS)

    The purpose of this study was twofold: first, to evaluate the myoblast labeling of various 99mTc complexes and to select the complex that best accomplishes this labeling, and second to evaluate the biodistribution of myoblasts labeled with this complex using mice with MDX muscular dystrophy (the murine homologue of Duchenne's muscular dystrophy). The following ligands were used to prepare the corresponding 99mTc complexes: hexakis-methoxy-isobutyl-isonitrile (MIBI), bis(2-ethoxyethyl)diphosphinoethane (Tf), (RR,SS)-4,8-diaza-3,6,6,9-tetramethyl-undecane-2,10-dione-bisoxime (HM-PAO), bis(N-ethyl)dithiocarbamate (NEt), and bis(N-ethoxy, N-ethyl)dithiocarbamate (NOEt). One million murine myoblasts were incubated for 30-60 minutes with 5 mCi of each of the 99mTc complexes prepared from the above ligands. Viability was assessed by microscopic counting after trypan blue staining, and the radioactivity absorbed in the cells was measured after centrifugation. The compound with the highest uptake in cellular pellets was [99mTc]N-NOEt. The biodistribution of myoblasts labeled with this complex was evaluated after intraaortic injection in dystrophic mice. Such an approach has the potential of effecting widespread gene transfer through the bloodstream to muscles lacking dystrophin

  12. Detection of axillary lymph node involvement of breast cancer by Tc-99m MIBI scintimammography

    International Nuclear Information System (INIS)

    Breast cancer is the most common malignancy among women, leading to hundreds of thousands of deaths annually around the world. Lymph node status is the most important prognostic indicator in newly diagnosed breast cancer. The presence of axillary lymph node metastases has major prognostic implications in breast cancer patients, and it is an important criterion in determining the need for adjuvant chemotherapy. There is not an accurate anatomical test for detecting axillary lymph node metastasis and clinical examination has inappropriate diagnostic values. Routine lymph node dissection is the only accepted method for therapeutic decisions but it is invasive and produces significant morbidity such as lymphedema and infections. On the other hand, an important proportion of breast cancer patients are nodenegative. Ultrasonography has also been reported to be helpful, especially in conjunction with fine needle aspiration biopsy

  13. The role of scintimammography with 99MTC-MIBI in evaluation of the breast lesions

    International Nuclear Information System (INIS)

    Introduction: The aim of this study was to determine diagnostic value of prone lateral 99mTc-MIBI scintigraphy in detection of primary breast cancer in patients with breast lesions. Materials and methods: We evaluated 142 patients with breast lesions and/or suspicious mammographic findings. In all patients, the diagnosis was established by pathology. Pattern of abnormal MIBI uptake ( focal or diffuse ) and quantitative measurement of the Ratio of Lesion to normal tissue uptake( T/N Ratio) was recorded. All lesions with abnormal focal uptake with T/N Ratio of more than 1.30 were considered as malignant lesion. Cases with normal homogeneous or abnormal diffusely increased uptake in the breast tissue were interpreted as negative for malignant lesion. Results: Of 142 patients, histopathologic study of 36 cases showed Malignancy which 34 cases of them had Positive MIBI scan. Of 106 cases of negative pathology ,cases had Negative MIBI scintimammography. Analysis of the findings showed high sensitivity, Specificity, accuray and Negative predictive value for 99m-MIBI scintimammography in detection of malignant breast lesion. Conclusion: We concluded that MIBI Scan can be used as complementary or even competitory imaging to the mammography in the evaluation of the breast lesions. (authors)

  14. Technetium-99m pyridoxylideneglutamate (P. G. ) cholescintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Stadalnik, R.C.; Matolo, N.M.; Jansholt, A.L.; Krohn, K.A.; DeNardo, G.L.; Wolfman, E.F Jr.

    1976-12-01

    Technetium-99m P.G. cholescintigraphy was performed in 27 human volunteers and 81 patients referred for hepatobiliary tract disease. The gallbladder, biliary system, and gastrointestinal tract were well visualized in the normal patients and volunteers. The gallbladder was not visualized in 22 patients with histologically proved cholecystitis with cystic duct obstruction. Nine patients with complete extrahepatic obstruction of the common bile duct were correctly diagnosed. Hepatocellular disease and incomplete obstruction, with and without jaundice, were diagnosed with this technique. Oral cholecystography is superior to this method for the detection of cholelithiasis in nonjaundiced patients.

  15. Evaluation of the effect of trimetazidine on Tc-99m methoxyisobutyl isonitrile gated scintigraphy in patients with coronary artery ectasia

    International Nuclear Information System (INIS)

    Coronary artery ectasia (CAE) is the abnormal dilatation of a segment of the coronary artery to a diameter of at least 1.5 times that of normal adjacent segments. Symptoms are variable, and its prognosis and treatment modalities are unclear. The aim of this study is to evaluate the effect of trimetazidine (TMZ) on ischemic left ventricular function by rest-stress Tc-99m methoxyisobutyl isonitrile (Tc-99m MIBI) myocardial scintigraphy in symptomatic patients with CAE. We included patients with ectasia admitted to our Cardiology Department, Turkey, between 2003 and 2004 in this study. All patients underwent coronary angiography and diagnosed with CAE, before and 4 weeks after TMZ administration. Seventeen patients (9 men, 8 women) underwent gated single-proton emission tomographic (SPECT) using Tc-99m MIBI. We performed quantitative global and regional ventricular functional analysis using quantitative gated SPECT software. The global ejection fraction increased from 59.9%+/-8.9% to 62.6%+/-8.3% after therapy (p=0.033). In addition, the end systolic volume and the end diastolic volume decreased after therapy (101.7+/-23.5 ml to 95.1+/-22.9 ml, p=0.002; from 41.1+/-14.3 to 36.4+/-13.6, p=002). In all segments, we observed significant post-therapy increases in relative tracer uptake. Percentage of MIBI uptake was 71.2+/-15.3 at baseline stress and 73.2+/-15 post-TMZ (p=0.001). As global function parameters, the total wall motion normal areas changed significantly (67-74% p=0.01) after therapy, but the total wall thickness did not changed significantly(49-45%, p=0.21). The results of this study demonstrate that TMZ improves myocardial function by rest-stress Tc-99m MIBI gated SPECT during stress-induced ischemia in patients with CAE. Also, the outcomes revealed improvement in functional parameters, and percentage of MIBI uptake post TMZ administration. We can use this procedure to monitor the effect of TMZ in CAE patients. (author)

  16. Comparison study among methodologies of planar chromatography for radiochemical control of technetium-99m

    International Nuclear Information System (INIS)

    Radiopharmaceuticals are substances that have radioisotopes in their composition. About 95% of the procedures performed in nuclear medicine use radiopharmaceuticals with diagnostic purposes, and the Lyophilized Reagents (LR) labeled with Technetium-99m (99mTc), obtained from 99Mo/99mTc generator, are the most one used. Quality Control represents the set of assays to be performed to assure that the product is adequate to its purpose. An important feature to be evaluated in 99mTc radiopharmaceuticals is the radiochemical purity (% RqP) to quantify free pertechnetate (99mTcO4-) and technetium colloidal (99mTcO2) mainly by paper chromatography (PC), thin layer (TLC) and High Performance Liquid Chromatography (HPLC). The objective of this work was to perform the comparison among the radiochemical control methodologies of LR labeled with 99mTc, described in the United States Pharmacopoeia (USP) and European Pharmacopoeia (EP) and those used by IPEN. 99mTcO4- eluate and DISIDA, DMSA, DTPA, EC, ECD, GHA, MIBI, MDP, PIRO, SAH and Sn Coloidal LR were provided by IPEN-CNEN/SP. TLC-cellulose, TLC-SG.TLC-SG reverse phase, HPTLC-cellulose, HPTLC-SG (Merck) and ITLC-SG (Pall Corporation), W1MM, W3MM, W17M e W31ET (Whatman) chromatographic plates were used. The measurement of the radioactivity was done in a Perkin Elmer Cobra D-5002 gamma counter. LR were labeled to obtain 55,0 MBq mL1 (1,5 mCi mL1) of final radioactive concentration. The %99mTcO4-, %99mTcO2 and % RqP were determined up to 4 hour labeling. From 11 LR, only EC and GHA have no radiochemical control methods in USP and EP. In USP and/or EP, DTPA, MDP, PIRO, SAH and Sn Coloidal methods use ITLC-SG; IPEN uses this chromatography plate in DISIDA, EC, ECD, GHA, PIRO, MIBI and SAH. As ITLC-SG had been out of production (recommended in 40, 70 and 41% of the USP, EP and IPEN methodologies, respectively), it was necessary to search alternatives to replace ITLC-SG plate in the radiochemical control, comparing with HPTLC

  17. Failed Switching off in the MIBI-Parathyroid Scintigraphy in a Dialyzed Patient with Secondary Hyperparathyroidism Responsive to Cinacalcet Therapy

    OpenAIRE

    Piergiorgio Bolasco; Alessandra Serra; Maurizio Loi; Andrea Galfré; Mario Piga

    2010-01-01

    The aims of your case report is to show the predictivity of Tc99m-sestamibi (MIBI) scintigraphy and doppler ultrasound imaging on secondary hyperparathyroidism (SHPT) in a patient responsive to calcimimetic treatment. Moreover, it has been reported that calcimimetic has great potential in reducing the volume of the parathyroid gland. On the other hand, the MIBI scintigraphy is considered a crucial diagnostic procedure to monitor the response to therapy in terms of turnover and cellular metabo...

  18. Diagnosis of parathyroid adenoma - the role of two phase mibi scintigraphy (abstract)

    International Nuclear Information System (INIS)

    Dual radionuclide imaging using a combination of /sup 201/Ti with/sup 99m/Tc pertechnetate is a useful procedure in the localization of parathyroid adenomas. Recently /sup 99m/Tc Sesta - Methoxy Isobutyle Isonitrile (MIBI) has been used for detection and localization of parathyroid adenoma in patients with hyperparathyroidism as a single radionuclide imaging procedure (Double phase study). In our study 4 female patients with picture of metabolic bone disease on bone scintigraphy and raised serum parathyroid hormone levels and raised levels of serum calcium, were subjected to two phase parathyroid MIBI scintigraphy. Cervicothoracic planar images (Anterior view) were acquired at 15-20 minutes and 2-4 hours after an intravenous injection of 15-20 mCi /sup 99m/Tc MIBI. A positive scan was defined as an area of increased focal uptake in normal tissue, which progressively decreased over time. All the 4 patients had a positive scan for parathyroid adenoma which was confirmed by histopathological examination of the excised tumor. It was concluded that the two phase 99mTc-MIBI parathyroid scintigraphy is a specific and sensitive modality for detection, localization and even diagnosis of parathyroid adenoma. (author)

  19. Use of 99mTc 2-methoxyisobutyl isonitrile in minimally invasive radioguided surgery in patients with primary hyperparathyroidism: A narrative review of the current literature

    International Nuclear Information System (INIS)

    The use of technetium-99m 2-methoxyisobutyl isonitrile (99mTc MIBI) for assistance in minimally invasive radioguided surgery (MIRS) is growing in popularity as a safe, effective, and proficient technique used for parathyroidectomy in primary hyperparathyroidism (PHPT) treatment. Previously, the preferred treatment for PHPT was bilateral neck exploration (BNE), a very invasive, costly, and lengthy procedure. However, as a large majority (80–85% of cases of PHPT) are attributed to a single parathyroid adenoma (PA), a simpler more direct technique such as MIRS is a far better option. The following article is an exploration of the current literature concerning varied protocols utilizing 99mTc MIBI for assistance in MIRS for patients undergoing treatment of PHPT. This technique boasts many advantageous outcomes for patients suffering from PHPT. These include a reduction in cost, operating time, and patient recovery; less evidence of post-surgical hypocalcaemia, less pain, and complications; superior cosmetic results; same-day discharge; and the possibility of local anaesthesia which is particularly beneficial in elderly patients. Better outcomes for patients with deep or ectopic PAs, reduced intra-operative complications, and improved cosmetic outcomes for patients who have previously undergone thyroid and/or parathyroid surgery are also advantageous. Of the literature reviewed it was also found that no patients suffered any major surgical complications such as laryngeal nerve palsy or permanent hypoparathyroidism using 99mTc MIBI for assistance in MIRS

  20. Use of {sup 99m}Tc 2-methoxyisobutyl isonitrile in minimally invasive radioguided surgery in patients with primary hyperparathyroidism: A narrative review of the current literature

    Energy Technology Data Exchange (ETDEWEB)

    Denmeade, Kristie A [Nuclear Medicine and Ultrasound Department, Bankstown-Lidcombe Hospital, Bankstown, New South Wales (Australia); Constable, Chris [Brain and Mind Research Institute, University of Sydney, New South Wales (Australia); Reed, Warren M [Discipline of Medical Radiation Sciences, Faculty of Health Sciences, The University of Sydney, New South Wales (Australia); Nuclear Medicine and Ultrasound Department, Bankstown-Lidcombe Hospital, Bankstown, New South Wales (Australia)

    2013-06-15

    The use of technetium-99m 2-methoxyisobutyl isonitrile ({sup 99m}Tc MIBI) for assistance in minimally invasive radioguided surgery (MIRS) is growing in popularity as a safe, effective, and proficient technique used for parathyroidectomy in primary hyperparathyroidism (PHPT) treatment. Previously, the preferred treatment for PHPT was bilateral neck exploration (BNE), a very invasive, costly, and lengthy procedure. However, as a large majority (80–85% of cases of PHPT) are attributed to a single parathyroid adenoma (PA), a simpler more direct technique such as MIRS is a far better option. The following article is an exploration of the current literature concerning varied protocols utilizing {sup 99m}Tc MIBI for assistance in MIRS for patients undergoing treatment of PHPT. This technique boasts many advantageous outcomes for patients suffering from PHPT. These include a reduction in cost, operating time, and patient recovery; less evidence of post-surgical hypocalcaemia, less pain, and complications; superior cosmetic results; same-day discharge; and the possibility of local anaesthesia which is particularly beneficial in elderly patients. Better outcomes for patients with deep or ectopic PAs, reduced intra-operative complications, and improved cosmetic outcomes for patients who have previously undergone thyroid and/or parathyroid surgery are also advantageous. Of the literature reviewed it was also found that no patients suffered any major surgical complications such as laryngeal nerve palsy or permanent hypoparathyroidism using {sup 99m}Tc MIBI for assistance in MIRS.

  1. Development and validation of methodology for technetium-99m radiopharmaceuticals using high performance liquid chromatography (HPLC)

    International Nuclear Information System (INIS)

    Radiopharmaceuticals are compounds, with no pharmacological action, which have a radioisotope in their composition and are used in Nuclear Medicine for diagnosis and therapy of several diseases. In this work, the development and validation of an analytical method for 99mTc-HSA, 99mTc-EC, 99mTc-ECD and 99mTc-Sestamibi radiopharmaceuticals and for some raw materials were carried out by high performance liquid chromatography (HPLC). The analyses were performed in a Shimadzu HPLC equipment, LC-20AT Prominence model. Some impurities were identified by the addition of a reference standard substance. Validation of the method was carried out according to the criteria defined in RE n. 899/2003 of the National Sanitary Agency (ANVISA). The results for robustness of the method showed that it is necessary to control flow rate conditions, sample volume, pH of the mobile phase and temperature of the oven. The analytical curves were linear in the concentration ranges, with linear correlation coefficients (r2) above 0.9995. The results for precision, accuracy and recovery showed values in the range of 0.07-4.78%, 95.38-106.50% and 94.40-100.95%, respectively. The detection limits and quantification limits varied from 0.27 to 5.77 μg mL-1 and 0.90 to 19.23 μg mL-1, respectively. The values for HAS, EC, ECD and MIBI in the lyophilized reagents were 8.95; 0.485; 0.986 and 0.974 mg L-1, respectively. The mean radiochemical purity for 99mTc-HSA, 99mTc-EC, 99mTc-ECD and 99mTc-Sestamibi was (97.28 ± 0.09)%, (98.96 ± 0.03)%, (98.96 ± 0.03)% and (98.07 ± 0.01)%, respectively. All the parameters recommended by ANVISA were evaluated and the results are below the established limits. (author)

  2. Possible role of 99mTc-Dextran (99mTc-Dx) in detection of occult small intestinal lesions

    International Nuclear Information System (INIS)

    Two cases are presented wherein 99mTc-dextran (99mTc-Dx) imaging localised focal pathology in the small intestine and contributed in reaching a preoperative diagnosis. In the first case, 99mTc-pertechnetate and 99mTc-Dx accumulated in a suspected Meckel's diverticulum equally well. 99mTc-Dx additionally identified exudative discharge from the lesion. In the second case, 99mTc-Dx identified a distal ileal lesion later proved to be a non-specific ulcer on surgery. 99mTc-Dx scanning may have a role in imaging of occult intestinal lesions. (author)

  3. Bioavailability of {sup 99m}Tc-paclitaxel-glucuronide ({sup 99m}Tc-PAC-G)

    Energy Technology Data Exchange (ETDEWEB)

    Biber Muftuler, F.Z.; Demir, I.; Uenack, P.; Ichedef, C.; Yurt Kilcar, A. [Ege Univ., Izmir (Turkey). Dept. of Nuclear Applications

    2011-07-01

    An antitumor agent paclitaxel (PAC) has been proved to be efficient in the treatment of breast and ovarian cancer. Glucuronic acid-derived paclitaxel compound (paclitaxel-glucuronide (PAC-G)) was enzymatically synthesized using microsome preparate separated from rat livers. The biodistribution mechanism of PAC-G in healthy female Albino Wistar rats has been investigated. The expected structure is confirmed according to LC/MS results, and the possible attachment is to C2-hydroxyl group. PAC-G was labeled with {sup 99m}Tc and the radiochemical yield of radiolabeled compound ({sup 99m}Tc-PAC-G) was 98.0 {+-} 02.74% (n=9). The range of the breast/blood and breast/muscle ratios is approximately between 3 and 35 in 240 min. All these experimental studies indicate that {sup 99m}Tc-PAC-G may potentially be used in breast tissue as an imaging agent. (orig.)

  4. Tc- 99 m gel generator; an alternative source of Tc-99 m for medical uses

    International Nuclear Information System (INIS)

    Tc-99 m is widespread radioisotope in nuclear medicine and it can be produced by two different methods. The first method is performed by chromatographic genera tory by using Mo-99 prepared by fission of U-235, and another method is activation of Mo-99 can be complexed by ZrOCl2 and produce a gel. Mo-99 remains in this gel and Tc-99 m elute with normal saline solution. Many factors such as P H, concentration of Mo in the gel structure, and drying conditions of gel affect on the efficiency of the generator. In this research the gel generator production and some factors which are effecting on the 99 m Tc production are presented

  5. Evaluation of Pakgen {sup 99m}Tc generators loaded with indigenous fission {sup 99}Mo

    Energy Technology Data Exchange (ETDEWEB)

    Mushtaq, A.; Pervez, S.; Hussain, S.; Mirza, J.A.; Asif, M.; Siddique, M.U.; Khalid, U.; Khan, B.; Khalid, M. [Pakistan Institute of Nuclear Science and Technology, Islamabad (Pakistan). Isotope Production Div.; Khan, M.M.

    2012-07-01

    Fission produced {sup 99}Mo/{sup 99m}Tc generators, called Pakgen, have been manufactured at the PINSTECH generator production facility since 2000 for nuclear medicine applications in Pakistan. These generators were loaded with fission {sup 99}Mo imported from NTP South Africa. Recently production of fission {sup 99}Mo has begun in the Molybdenum-99 Production Facility at PINSTECH. Prior to use in the clinic, eight sets of generators were produced, 23 GBq and 74 GBq at time of calibration, and they were subjected to various quality control procedures recommended in the pharmacopoeia to assess their performance. The elution profile, volume, activity, pH, radionuclidic, chemical, radiochemical and biological purity, and expiry time of the eluates were examined. Labeling efficiency tests were also carried out with a number of the more widely used in vivo radiopharmaceutical kits like DTPA, MDP and MIBI. Performance of {sup 99m}Tc generators loaded with locally produced and imported fission {sup 99}Mo was comparable, and the users of Pakgen generators were fully satisfied. The effect of a wet vs. a dry column on {sup 99m}Tc yields of generators loaded with low and high activity was also studied for the first time. (orig.)

  6. Myocardial {sup 99m}Tc-sestamibi extraction and washout in hypertensive heart failure using an isolated rat heart

    Energy Technology Data Exchange (ETDEWEB)

    Fukushima, Kenji [Department of Cardiology, Tokyo Women' s Medical University, Tokyo 162-8666 (Japan); Department of Radiology, Johns Hopkins University, Baltimore, MD (United States); Momose, Mitsuru, E-mail: mmomose@rad.twmu.ac.j [Department of Diagnostic Imaging and Nuclear Medicine, Tokyo Women' s Medical University, Tokyo 162-8666 (Japan); Kondo, Chisato [Department of Diagnostic Imaging and Nuclear Medicine, Tokyo Women' s Medical University, Tokyo 162-8666 (Japan); Higuchi, Takahiro [Department of Radiology, Johns Hopkins University, Baltimore, MD (United States); Kusakabe, Kiyoko [Department of Diagnostic Imaging and Nuclear Medicine, Tokyo Women' s Medical University, Tokyo 162-8666 (Japan); Hagiwara, Nobuhisa [Department of Cardiology, Tokyo Women' s Medical University, Tokyo 162-8666 (Japan)

    2010-11-15

    Purpose: Myocardial mitochondria are the primary part of energy production for healthy cardiac contraction. And mitochondrial dysfunction would play an important role in progressive heart failure. In the recent years, myocardial washout of {sup 99m}Tc-sestamibi [({sup 99m}Tc-hexakis-2-methoxy-2-methylpropyl isonitrile (MIBI)] has been introduced to be a potential marker in patients with heart failure. The objective of this study was to clarify MIBI extraction and washout kinetics using isolated perfusion system in hypertension induced model of myocardial dysfunction. Methods: Six-week-old Dahl-salt sensitive rats, allotted to 4 groups; a 5-week high-salt group (5wk-HS), 12-week high-salt group (12wk-HS) and two age-matched, low-salt diet control groups (5wk-LS and 12wk-LS). The rats in 5wk-HS and 12wk-HS groups were fed a high-salt diet (containing 8% NaCl). Cardiac function was examined by echocardiography before removing heart. Hearts were perfused according to the Langendorff method at a constant flow rate, in which 20-min MIBI washin was conducted followed by 25-min MIBI washout. Whole heart radioactivity was collected every sec by an external gamma detector. The myocardial extraction, K{sub 1} (ml/min) and washout rate, k{sub 2} (min{sup -1}) were generated. Results: High-salt diet groups showed significant high-blood pressure. Echocardiography revealed thickened LV walls in 5wk-HS, and reduced cardiac function in 12wk-HS, compared to each age-matched control group. K{sub 1} showed no significant difference among all groups (5wk-HS: 2.36{+-}1.07, 5wk-control: 2.59{+-}0.28, 12wk-HS: 1.91{+-}0.90, and 12wk-control: 2.84{+-}0.57). k{sub 2} in 5wk-HS was comparable to that in the age matched control group (0.00030{+-}0.00039 vs -0.000010{+-}0.00044), but it was increased remarkably in 18wk-HS compared to the age matched control group (0.0025{+-}0.0011 vs 0.000025{+-}0.000041, P<.01), and 5wk-HS (P<.01). Conclusion: In the course of hypertensive heart disease, MIBI

  7. 99mTc-sestamibi scintimammographic evaluation of the mammae for carcinoma

    International Nuclear Information System (INIS)

    Full text: Introduction: The value of 99m Tc-sestamibi (MIBI) in the detection of breast carcinoma has been reported extensively, but the extent to which it might complement conventional mammography demands more studies. Although an excellent diagnostic intervention, the sensitivity of mammography decreases to less than 70% in women with radiologically dense breast tissue. The sensitivity of scintimammography, unlike standard screening modalities, is not compromised by inherent breast density. The incidence of breast cancer varies significantly among racial/ethnic groups and apparently Negroid women have higher blood estrogen levels than their Caucasian counterparts. Considering the racial, cultural and socio-economic differences of the South African population, as well as the relative simple technology and cost effectiveness of MIBI scintimammography, the question is: should scintimammography not play a more prominent role as rule-out diagnostic protocol in the evaluation of the breasts for carcinoma? Materials and methodology: Fifty-three consecutive patients presenting to the surgical out-patient department with or without breast nodules but high risk category for breast carcinoma have been recruited. After taking a history and performing a physical examination patients were referred for scintimammography and some of the patients were also referred for radiological mammography. Fine needle aspiration (FNA) was performed on all the lesions. Results: Clinical evaluation: sensitivity: 88,89%; specificity: 88.64%; false +ve: 4; false -ve :1. Sonographic evaluation: sensitivity: 100%; specificity: 33.35%; false +ve: 4; false -ve :0. MIBI scintimammography evaluation: sensitivity: 90.91%; specificity: 90,48%; false +ve: 4; false -ve: 1. Mammographic evaluation: sensitivity: 100%; specificity: 45,5%; false +ve: 6; false -ve: O. Fine needle aspiration cytology: sensitivity: 100%; specificity: 95,35%; false +ve: 2; false -ve: 1. Conclusion: Interesting as the results may

  8. Preparation and assessment of [99mTc]technetium aquacarbonyl complexes with 1,2-diaminoethane-N-substituted ligands for tumor detection

    International Nuclear Information System (INIS)

    Over least 15 years the complex [[99mTc](H20)3(CO)3]+ has been used as an intermediary to obtain technetium radiopharmaceuticals for applications in cardiology, neurology and oncology. Two important characteristics of this molecule are: the facility for obtaining that compound from aqueous solutions and the easiness of substituting H2O molecules by atoms of other ligand molecules. In this project we prepared new complexes [[99mTc](CMNS001-3)(H2O)(CO)3]+, where (CMNS001) = N-[(4-methoxy) benzyl]-1,2-diaminoethane, (CMNS003) = N,N'-bis-[(4-methoxy)benzyl]-1,2-diaminoethane, and assessed the uptake of these complexes in murine melanoma cancer cell B16F10 and breast cells MCF-7 and MDA-MD-231, and compared with [[99m](MIBI)6]+ uptake. In vitro uptake for both new technetium complex reached values close to 5%, for all cell lines, whereas the [[99mTc](MIBI)6]+ uptake was close to 1 %. The assessment of subcellular distribution showed high accumulation of the new complex in the membrane fraction, for MDAMB-231, while for B16F10 accumulation occurred both in membrane and cytoplasm; the concentration of [[99mTc](MIBI)6]+ was mainly in the cytoplasm portion. Biodistribution study in mice allowed to observe the capture of up to 1.6% of the administered dose per gram of tumor tissue for the complex [[99mTc](CMNS001)(H2O)(CO)3]+, whereas other organs such as heart, lung and muscle, showed uptake of about 5.6%, 6.4% and 2%, respectively. The complexes in this work showed a high rate of uptake in vitro, but was not reproduced in vivo model, which can be related to low concentration of the complexes inside the cells and reduced vascularity of tumor tissue, with lower intake of complex through the blood system. (author)

  9. Process modifications of obtaining Tc-99m by solvent extraction

    International Nuclear Information System (INIS)

    This paper describes a modification in the process to obtaining Tc-99m by the extraction method of solvent from Mo-99 produced by irradiation. Tc-99m is considered an ideal radionuclide for medical and biological applications

  10. MIBI scintigraphy in hypofunctioning thyroid nodules. Can it predict the dignity of the lesion?

    Energy Technology Data Exchange (ETDEWEB)

    Theissen, P.; Schmidt, M.; Ivanova, T.; Dietlein, M.; Schicha, H. [Dept. of Nuclear Medicine, Univ. of Cologne (Germany)

    2009-07-01

    Aim: Several authors have investigated the value of technetium-MIBI scanning to predict the dignity of hypofunctioning, cold thyroid nodules (HTN) in regions with differing levels of iodine supply. They concluded that an MIBI scan can exclude thyroid malignancy, although comparisons between the studies are of limited value owing to differences in methodology and wide variations in patient selection criteria. The present study investigates whether the above claim is also valid in Germany, a country with a long-standing and persistent, mild iodine deficiency and a high incidence of nodules in large goiters with a low prevalence of malignancy. Patients, methods: The study compares the results of {sup 99m}Tc-MIBI scintigraphy (incl. SPECT and planar images) in HTN (MIBI) with those of fine-needle aspiration biopsy (FNAB) and histology. Of 154 consecutive patients (121 women, 33 men; mean age 56 {+-} 12 years), 73 underwent thyroid surgery from which the results of FNAB and MIBI were assessed. Selection criteria were risk estimation or conditions limiting the feasibility of FNAB. The mean thyroid volume was 42 {+-} 25 ml, with 2.1 {+-} 1.4 nodules per patient. Results: Histology revealed thyroid malignancies in 8 out of 73 patients (11.0%). The negative predictive value for MIBI was 97%, which is comparable to FNAB (94%). However, in 19.5% of patients FNAB was indeterminate. Lower specificity (54%) and low positive predictive value (19%) showed that MIBI accumulation cannot differentiate between malignant and benign thyroid nodules. However, comparison with cytological and/or histological findings indicated that it could distinguish between lesions with differing rates of mitochondrial metabolism. Conclusion: Even in areas of former or mild iodine deficiency MIBI forms a basis for choosing between wait-and-see and surgical strategies if FNAB is unfeasible or not representative. However, even for pathological MIBI results, the prevalence of malignancy is not very high

  11. Current status of Tc-99m production in Thailand

    Energy Technology Data Exchange (ETDEWEB)

    Charoen, Sakda [Office of Atomic Energy for Peace, Bangkok (Thailand)

    2003-03-01

    Technetium-99m is the workhorse of nuclear medicine and currently accounts for over 80% of all in vivo diagnostic procedures. In Thailand, Office of Atomic Energy for Peace (OAEP) had experiences in production of Technetium-99m by solvent extraction process and alternative technology for Technetium-99m generator based on zirconium molybdate and titanium molybdate gel generators were also studied. The paper describes past experiences and future plan of Technetium-99m production in Thailand. (author)

  12. Technetium-99m-dimethylglyoxime ([sup 99m]Tc-DMG) as renal imaging agent

    Energy Technology Data Exchange (ETDEWEB)

    Adonaylo, V.N. (Buenos Aires Univ. (Argentina). Facultad de Ciencias Exactas y Naturales Buenos Aires Univ. (Argentina). Dept. de Ciencias Biologicas); Stahl, Adriana; Pomilio, A.B.; Vitale, A.A. (Buenos Aires Univ. (Argentina). Facultad de Ciencias Exactas y Naturales); Canellas, C.O. (Buenos Aires Univ. (Argentina). Facultad de Ciencias Exactas y Naturales Comision Nacional de Energia Atomica, Buenos Aires (Argentina))

    1993-06-01

    Dimethylglyoxime (DMG) labelled with [sup 99m]Tc is presented as a renal imaging agent. The behaviour of this complex was analysed at different pH by means of UV spectral data and using DMG-calcium chloride as a reference complex. Biokinetic data were evaluated in two biological models, Sprague-Dawley rats and Didelphis albiventris argentine opossum. Biodistribution in rats demonstrated fast and specific renal excretion. Time-activity values over both kidneys could be quantified for this complex. Renographic studies led to mean time-to maximum values on twelve assays of 2.0 [+-] 0.1 min and a mean relative function of 53.0 [+-] 2.3 and 47.0 [+-] 3.2 for right and left kidneys, respectively. [sup 99m]Tc-DMG showed specificity for the renal excretion pathway and therefore seems to be a very useful radiopharmaceutical for renal function studies. (Author).

  13. Technetium-99m-dimethylglyoxime (99mTc-DMG) as renal imaging agent

    International Nuclear Information System (INIS)

    Dimethylglyoxime (DMG) labelled with 99mTc is presented as a renal imaging agent. The behaviour of this complex was analysed at different pH by means of UV spectral data and using DMG-calcium chloride as a reference complex. Biokinetic data were evaluated in two biological models, Sprague-Dawley rats and Didelphis albiventris argentine opossum. Biodistribution in rats demonstrated fast and specific renal excretion. Time-activity values over both kidneys could be quantified for this complex. Renographic studies led to mean time-to maximum values on twelve assays of 2.0 ± 0.1 min and a mean relative function of 53.0 ± 2.3 and 47.0 ± 3.2 for right and left kidneys, respectively. 99mTc-DMG showed specificity for the renal excretion pathway and therefore seems to be a very useful radiopharmaceutical for renal function studies. (Author)

  14. Kinetic investigations of sup(99m)Tc-labelled radiopharmaceuticals

    International Nuclear Information System (INIS)

    Several radiopharmaceuticals (sup(99m)Tc-Fe-ascorbates, sup(99m)Tc-Sn-DTPA, sup(99m)Tc-Sn-ACD-citrate-complex and sup(99m)Tc-Sn-tetracyclin-HAsc-ACD-complex) for renal and tumour scintigraphy were tested in animal experiments. Also tested was sup(99m)Tc-penicillamine for scintigraphic investigations of the gallbladder and the liver. The findings suggest that the different radiopharmaceuticals have different degrees of reliability and exactness, and that some of them should be combined to achieve better diagnostic values. (GSE/AK)

  15. The role of Sesta-Mibi-SPECT in angiographically documented coronary heart disease

    International Nuclear Information System (INIS)

    131 patients were studied to compare the results of 99mTc-Mibi-SPECT with coronary angiography (CAG). 127 patients remained for final evaluation. A scar was falsely diagnosed in 17. 13 patients had a normal CAG and pathologic Sesta-Mibi-SPECT. 5 of them suffered from cardiomyopathy. 2 patients have had a myocarditis, at 2 others a PTCA was perfomred. 1 patient had a 20% LCA-stenosis and another one a left ventricular hypertrophy. Two times the reason for the pathologic Sesta-Mibi-SPECT could not be found. 1 patient had a normal Sesta-Mibi-SPECT despite of a pathologic CAG, angina pectoris and pathologic ECG. We got congruent results in 113 patients. 7 patients showed a left bundle branch block (LBBB), in 5 of them the Mibi-SPECT corresponded well with the CAG. 2 patients with dilated cardiomyopathy and no CHD showed an exercise induced ischemia in the anteroseptal wall. In patiens with angiographically documented CHD Sesta-Mibi-SPECT gives reliable diagnostic information concerning the myocardial perfusion. (orig./MG)

  16. GAMMAGRAFÍA CON 99TC-MIBI PARA DETERMINAR LA EFECTIVIDAD DE LA HEBERQUINASA PARA REPERFUNDIR LA ARTERIA RELACIONADA CON EL INFARTO / 99tc-mibi gammagraphy to determine Heberkinase effectiveness in the reperfusion of the infarction-related artery

    Directory of Open Access Journals (Sweden)

    Marilyn Ramírez Méndez

    2010-12-01

    Full Text Available Resumen Introducción y objetivos: La utilización de técnicas nucleares para la determinación de la permeabilidad de la arteria relacionada con el infarto, es de gran importancia clínica. El objetivo de esta investigación fue conocer el estado de la perfusión miocárdica del territorio dependiente de la irrigación de esa arteria y determinar la presencia o no de su reapertura después de la trombólisis. Método: Se realizó una investigación experimental puntual con una muestra de 10 pacientes consecutivos, de ambos sexos y cualquier edad, que ingresaron en la Unidad de Cuidados Intensivos del Hospital Universitario "Dr. Celestino Hernández Robau" de Santa Clara, con el diagnóstico clínico y electrocardiográfico de infarto agudo de miocardio a los que se les administró tratamiento trombolítico con Estreptoquinasa Recombinante Cubana. Resultados: El 60 % pertenece al sexo masculino y el 70 % de la muestra presentaba edades superiores a los 50 años. Fue más frecuente la localización inferior del infarto (50 %, y el 70 % de la serie recibió tratamiento trombolítico en las primeras 6 horas de evolución. La evaluación gammagráfica de la perfusión miocárdica demostró 12 defectos de captación, la tercera parte de ellos (33,3 % se consideraron leves. Después de la aplicación del tratamiento trombolítico hubo una reducción del 50 % de los defectos de captación. Conclusiones: El estudio gammagráfico con 99Tc-MIBI fue útil para demostrar la recanalización de la arteria relacionada con el infarto, tras la utilización de tratamiento trombolítico con Estreptoquinasa Recombinante Cubana. / Abstract Introduction and objectives: Of great clinical importance is the use of nuclear techniques for determining the permeability of the infarction-related artery. The objective of this research was to know the myocardial perfusion state of the area dependent on the irrigation of this artery and to determine whether or not a reopening

  17. Localizacao de glandulas paratireoides ectopicas e supranumerarias em pacientes com hiperparatireoidismo secundario e terciario: descricao cirurgica e correlacao com ultrassonografia e cintilografia Tc99m-Sestamibi pre-operatorios

    Directory of Open Access Journals (Sweden)

    Jose Santos Cruz de Andrade

    2014-01-01

    Full Text Available Introdução: O hiperparatireoidismo é uma consequência metabólica esperada na doença renal crônica (DRC. Paratireoides (PT ectópicas e/ou supranumerárias podem ser causa de falha cirúrgica nos pacientes submetidos à paratireoidectomia total (PTX. Objetivo: Definir cirurgicamente a localização das PT, em pacientes com hiperparatireoidismo associado à DRC, e correlacionar esses achados com os exames pré-operatórios. Materiais e métodos: Foi conduzido um estudo retrospectivo com 166 pacientes submetidos à PTX. A localização das PT no intraoperatório foi registrada, sendo classificada como tópica ou ectópica. A localização pré-operatória, definida pela ultrassonografia (USG e pela cintilografia Tc99m-Sestamibi (MIBI, foi comparada com aos achados cirúrgicos. Resultados: Nos 166 pacientes, foram identificadas 664 PT. Foram classificadas como tópicas e ectópicas 577 (86,4% e 91(13,6% glândulas, respectivamente. Oito PT supranumerárias foram encontradas (7 tópicas e 1 ectópica. As localizações mais comuns de PT ectópicas foram as regiões retroesofágica e tímica. Associadas, a USG e a MIBI não identificaram 56 glândulas (61,5% ectópicas. Entretanto, a MIBI foi positiva para 69,7% daquelas localizadas nas regiões tímicas e mediastinal. Conclusão: A presença de glândulas ectópicas e supranumerárias em pacientes com hiperparatireoidismo associado à DRC é significativa. Os exames de imagem pré-operatórios não localizaram a maioria das glândulas ectópicas. A MIBI pode ter importância na identificação de PT nas regiões tímica e mediastinal.

  18. Retention of 99mTc-DMSA(III) and 99mTc-nanocolloid in different syringes affects imaging quality.

    Science.gov (United States)

    Bauwens, Matthias; Pooters, Ivo; van der Pol, Jochen; Mottaghy, Felix M; van Kroonenburgh, Marinus

    2014-04-01

    (99m)Tc-dimercaptosuccinic acid [DMSA(III)] and colloidal human serum albumin ((99m)Tc-nanocolloid) are widely used radiopharmaceuticals. Recently, in our institution we encountered image quality problems in DMSA scans after changing the brand of syringes we were using, which triggered us to look into the adsorption properties of syringes from different brands for (99m)Tc-DMSA(III) and (99m)Tc-nanocolloid. We also describe a clinical case in which adsorption of (99m)Tc-DMSA(III) caused inferior imaging quality. DMSA and nanocolloid were labeled with (99m)Tc following manufacturer guidelines. After synthesis, syringes with (99m)Tc-DMSA(III) and (99m)Tc-nanocolloid were stored for 15, 30, 60, and 120 min. We evaluated Luer Lock syringes manufactured by different brands such as Artsana, Henke-Sass-Wolf, B. Braun Medical N.V., CODAN Medizinische Geräte GmbH & Co KG, Becton Dickinson and Company, and Terumo Europe. Adsorption of (99m)Tc-DMSA(III) and (99m)Tc-nanocolloid was acceptably low for all syringes (brands with (99m)Tc-DMSA(III) adsorption rates of 36 and 30%, respectively, and for one brand with a (99m)Tc-nanocolloid adsorption rate of 27%. Adsorption of (99m)Tc-DMSA(III) and (99m)Tc-nanocolloid reaches critical levels in syringes produced by two brands, potentially causing poor image quality--for example, in DMSA scans using pediatric radiopharmaceutical doses. It is advised to check the compatibility of any radiopharmaceutical with syringes as an integral part of the quality assurance program. PMID:24569706

  19. Biodistribution studies of {sup 99m}Tc-labeled myoblasts in a murine model of muscular dystrophy

    Energy Technology Data Exchange (ETDEWEB)

    Colombo, F.R. E-mail: colombof@policlinico.mi.it; Torrente, Y.; Casati, R.; Benti, R.; Corti, S.; Salani, S.; D' Angelo, M.G.; DeLiso, A.; Scarlato, G.; Bresolin, N.; Gerundini, P

    2001-11-01

    The purpose of this study was twofold: first, to evaluate the myoblast labeling of various {sup 99m}Tc complexes and to select the complex that best accomplishes this labeling, and second to evaluate the biodistribution of myoblasts labeled with this complex using mice with MDX muscular dystrophy (the murine homologue of Duchenne's muscular dystrophy). The following ligands were used to prepare the corresponding {sup 99m}Tc complexes: hexakis-methoxy-isobutyl-isonitrile (MIBI), bis(2-ethoxyethyl)diphosphinoethane (Tf), (RR,SS)-4,8-diaza-3,6,6,9-tetramethyl-undecane-2,10-dione-bisoxime (HM-PAO), bis(N-ethyl)dithiocarbamate (NEt), and bis(N-ethoxy, N-ethyl)dithiocarbamate (NOEt). One million murine myoblasts were incubated for 30-60 minutes with 5 mCi of each of the 99mTc complexes prepared from the above ligands. Viability was assessed by microscopic counting after trypan blue staining, and the radioactivity absorbed in the cells was measured after centrifugation. The compound with the highest uptake in cellular pellets was [{sup 99m}Tc]N-NOEt. The biodistribution of myoblasts labeled with this complex was evaluated after intraaortic injection in dystrophic mice. Such an approach has the potential of effecting widespread gene transfer through the bloodstream to muscles lacking dystrophin.

  20. Sup(99m)Tc compounds for diagnostic purposes

    International Nuclear Information System (INIS)

    The applications of sup(99m)Tc in nuclear medicine are discussed, such as sodium pertechnetate in thyroid and brain scintigraphy, complex compounds in lungs and liver diao.nosis. Technetium generators are classified according to the method of separating sup(99m)Tc from 99Mo. Adsorption generators are used, molybdate-99 is adsorbed on an Al2O3-packed column while pertechnetate-99m is eluted with 0.9% NaCl solution. Also used is continuous pertechnetate-99m extraction with methyl ethyl ketone from 0.5 M potassium molybdate and 2.5 M of potassium carbonate. The manufacture is described of kits for sup(99m)Tc radiopharmaceuticals preparation, eg., Diagos I, a gluconate complex, a lyophilisate for sup(99m)Tc-sodium pyrophosphate injections, a diagnostic kit for lung scintiscanning. (H.S.)

  1. 99mTc-甲氧基异丁基异腈显像对乳腺肿物及腋淋巴结转移的诊断%99mTc-methoxyisobutyl isonitrile imaging in identification of benign and malignant breast lesions and detection of metastatic axillary lymph node

    Institute of Scientific and Technical Information of China (English)

    张致峰; 毕建威; 孔令山; 张晏玲; 苏金莲; 梁丽

    2001-01-01

    Objective: To identify benign and malignant breast lesions and detect metastatic axillary lymph nodes using99mTc-methoxyisobutyl isonitrile(MIBI) imaging. Methods: Fifty-one women with a palpable breast lesions and 11 patients suspicious of metastatic lymph node after primary breast cancer operation were studied with planar imaging 10 min and 60 min after injection of 99mTc-MIBI. Semiquantitative analysis of uptake ratio(UR) of 99mTc-MIBI in locations of tumour and lymph nodes were performed. All the results were compared with pathologic study. Results: In palpable lesions, the sensitivity of 99mTc-MIBI planar imaging for detecting primary breast cancer was 93.3% and the specificity was 95.2%;The positive predictive value was 96.6%. The negative predictive value was 90.9%. The sensitivity of 99mTc-MIBI planar imaging to detect metastatic axillary lymph nodes was 64.0% and the specificity was 94.1%;The positive predictive value was 94.1%. The negative predictive value was 64.0%. Conclusion: 99mTc-MIBI planar imaging has high sensitivity and specificity for differentiation of breast tumors and detection of axillary lymph node involvement.%目的:研究99mTc-甲氧基异丁基异腈(99mTc- MIBI)显像鉴别诊断乳腺肿物的良、恶性及探测腋淋巴结转移的情况。方法:对51例可触及的乳腺肿物及11例乳腺癌术后疑腋淋巴结转移患者分别于注射99mTc-MIBI后10和60 min行平面显像,半定量分析肿物部位的摄取比值(uptake ratio,UR),均与病理结果对比。结果:99mTc-MIBI平面显像对可触及的乳腺肿物诊断乳腺癌的敏感性为93.3%,特异性为95.2%;阳性预测值为96.6%,阴性预测值为90.9%。探测腋淋巴结转移的敏感性为64.0%,特异性为94.1%;阳性预测值为94.1%,阴性预测值为64.0%。结论:99mTc-MIBI平面显像鉴别乳腺肿物的良、恶性及探测腋淋巴结转移有较高的敏感性和特异性。

  2. {sup 99m}Tc sestamibi myocardial perfusion scintigraphy with the novel use of metamizol for the detection of perfusion reversibility

    Energy Technology Data Exchange (ETDEWEB)

    Erguen, Eser Lay; Caglar, Meltem; Bozkurt, Murat Fani [Hacettepe University, Department of Nuclear Medicine, School of Medicine, Ankara (Turkey); Erguen, Hakan [Ankara University, Department of Pharmacology and Clinical Pharmacology, Faculty of Medicine, Ankara (Turkey)

    2008-08-15

    This study aims to investigate whether induction with metamizol, an analgesic-antipyretic drug having spasmolitic activity, could be used to increase the detectability of ischemic/jeopardized myocardium during MPS (myocardial perfusion scintigraphy). Metamizol-enhanced rest MPS (45 min after administration of 1 g metamizol orally, 740 MBq {sup 99m}Tc sestamibi was injected, MPS was acquired 45 min later) was performed in 21 patients who had perfusion defects on their previous stress-rest {sup 99m}Tc sestamibi MPS. Blood pressure was monitored at 15-min intervals. Stress, rest, metamizol-rest MPS images were interpreted on the model of 20 segments using a visual uptake score (VUS; 0 = normal, 1 = mild, 2 = moderate, 3 = significant decreases, 4 = no uptake). {sup 99m}Tc sestamibi uptake ratios (MIBI-UR; mean counts in the region of the perfusion defect/mean counts in the region of the normal-perfused wall) were obtained on each MPS and compared with each other. Average MIBI-UR in each scintigraphic examination was calculated. MPS were compared with coronary angiography results. VUS and MIBI-UR results showed that metamizol-rest MPS displayed the defect reversibility better than rest MPS. Of the 14 segments with fixed perfusion defects on stress-rest MPS, 8 showed improvement of perfusion after metamizol induction. In 33 segments, lesion reversibility was better delineated on metamizol-rest MPS. Metamizol-induced sestamibi uptake was significantly higher (p < 0.001) than stress/baseline rest examinations as calculated by the MIBI-UR. Blood pressure remained unaltered. Coronary angiography results were in concordance with metamizol induced MPS. Metamizol-enhanced rest MPS increases detectability of ischemic/viable myocardium during MPS. Metamizol should be discontinued like nitrates before stress MPS since it may mask the visualization of ischemic perfusion defects. (orig.)

  3. Influence of background correction in the estimation of myocardial uptake of 99mTc labelled perfusion imaging agents

    International Nuclear Information System (INIS)

    The effects of different corrections for background activity in the estimation of low organ uptake of radiopharmaceuticals have been examined using myocardial perfusion imaging agents. Estimates of myocardial uptake of 99mTc-labelled MIBI and tetrofosmin were made both at rest and after exercise. Patients were given one or other of the agents (12 MIBI; 17 tetrofosmin) and the measurements at rest and after exercise were made within a week of each other using a planar gamma camera method incorporating an attenuation-corrected, geometric mean technique. Myocardial uptakes were estimated using two different background corrections and also with no background subtraction. Mean values were in the range 1.3 to 3.0% and showed that, for both agents, uptakes estimated with and without background correction could differ by a factor of two. Although the study was not designed to compare myocardial uptakes of the two agents, a background correction which accounted separately for activity in tissue over- and under-lying the heart resulted in similar mean values for tetrofosmin (1.7% both at rest and after exercise) and for MIBI (1.8% rest; 1.9% exercise). For both agents, no significant difference was observed between myocardial uptakes at rest and after exercise measured at about two hours post-injection. (author) The effects of different corrections for background activity in the estimation of low organ uptake of radiopharmaceuticals have been examined using myocardial perfusion imaging agents. Estimates of myocardial uptake of Tc-labelled MIBI and tetrofosmin were made both at rest and after exercise. Patients were given one or other of the agents (12 MIBI; 17 tetrofosmin) and the measurements at rest and after exercise were made within a week of each other using a planar gamma camera method incorporating an attenuation-corrected, geometric mean technique. Myocardial uptakes were estimated using two different background corrections and also with no background subtraction

  4. 99mTc-ciprofloxacin for diagnosis of bacterial infection

    Science.gov (United States)

    Aungurarat, A.; Ngamprayad, T.; Dangprasert, M.; Phumkem, S.; Jowanaridhi, B.

    2015-05-01

    Preparation of 99mTc-ciprofloxacin for diagnosis of bacterial infection was investigated by varying factors which affected this compound. The optimum conditions for preparation of 99mTc-ciprofloxacin and a lyophilized kit for Tc-99m labelling were studied. The results from biodistribution study showed that the percentages of the injected dose per gram tissues of infected area at 1 and 3 hours after injection were around 0.25-0.56. 99mTc-ciprofloxacin was found sterile, pyrogen-free and non-toxic. Radiochemical purity was greater than 90% with greater than 6 hours of stability.

  5. Imaging bone infection using Tc-99m infecton: case studies

    International Nuclear Information System (INIS)

    Tc-99m infecton (ciprofloxacin) was introduced recently as an infection imaging agent which is specific for bacteria. If successful, this may prove superior to labelled leucocyte imaging, antibodies and other nonspecific agents in terms of logistics, cost and efficacy. We had recently acquired few kits for Tc-99m infecton preparation. Our preliminary experience suggests that it has high specificity for the infected bone and may be useful in bone tuberculosis. Three cases are presented where both Tc-99m MDP scanning and Tc-99m infecton study were undertaken to highlight the potential of this new agent. (author)

  6. Tc99m-Sestamibi: Development of Radiopharmaceutical Kit For Heart Imaging

    International Nuclear Information System (INIS)

    A development and comparison study of the Nuclear Malaysia's Tc99m-sestamibi heart imaging agents (NM kits) and the commercial products is reported. Three batches of kits labelled as B1, B2 and B3 were produced by freeze drying technique. The commercial products such as sestamibi Cardiolite, Polatom, ChiMIBI and tetrofosmin Myoview were used in this study. The quality control testing which included microbiology testing, radiochemical and animal biodistribution study were conducted accordingly. The NM sestamibi kits passed the sterility and pirogen test. The Radiochemical Purity testing (RCP) was assessed by the Instant Thin Layer Chromatography (ITLC) and High-Performance Liquid Chromatography (HPLC) methods after the reconstitution of the Tc99m-sestamibi. The RCP results were above 90 % and the kits were stable for 52 weeks. The animal biodistribution studies were carried out on Sprague-Dawley rats at 5, 30, 60, 120 and 1440 minutes post injection time intervals. The percentage injected dose per gram organ in heart for Tc99m-B1 were 4.722 ± 0.343 %, 3.752 ± 0.438 %, 4.564 ± 0.664 %, 4.180 ± 1.293 % and 1.090 ± 0.230 % at 5, 30, 60, 120 and 1440 minutes respectively. This is followed by the Tc99m-B2; 3.852 ± 0.406 %, 3.268 ± 0.425 %, 3.366 ± 0.316 %, 4.324 ± 1.044 % and 1.038 ± 0.144 %, Tc99m-B3; 5.404 ± 0.351 %, 4.818 ± 0.579 %, 6.015 ± 0.774 %, 5.028 ± 1.353 % and 1.623 ± 0.692 % at the same time intervals. Independent T Test showed that heart uptake was significant as compared to the control (p<0.05). The biodistribution study also showed that the radiopharmaceuticals localized selectively in the myocardium of rats. The NM sestamibi kits were comparable to the commercial products. Blood, lung, kidney and intestines washout were found to be fast and efficient. The biodistribution and analysis of heart-to-lung and heart-to-liver uptake ratios showed that NM sestamibi kits has a potential and could be used as heart imaging agents. (author)

  7. The radiopharmaceuticals labelled with technetium-99m and the radiopharmacy; Les radiopharmaceutiques marques au technetium-99m et la radiopharmacie

    Energy Technology Data Exchange (ETDEWEB)

    Bodenant, V

    1998-10-01

    In less than fifty years, the place of nuclear medicine is become primordial. Among all the radiopharmaceuticals used in nuclear medicine, the technetium-99m is the most used because of its physico-chemical properties and its great availability with the molybdenum-99m - technetium-99m generator. Since 1992, the radiopharmaceuticals, the packages, the generators are included in the pharmaceutic monopole. They are now under the reliability of the radio-pharmacist. This thesis has for object to introduce these different radiopharmaceuticals labelled with technetium-99m and to show the primordial place of the radio-pharmacist in a service of nuclear medicine. (N.C.)

  8. Comparative scintigraphic study of renal function with 99mTc-Mercaptoacetyl-triglycine and 99mTc-Ethylenedicysteine

    OpenAIRE

    Beiki D.; Eftekhari M; Fallahi B.; Gheisari F.; Hozhabrosadati M.; Fard-Esfahani A.; Takavar A; Gholamrezanezhad A.; Saghari M. Ansari Gilani K.

    2008-01-01

    99mTc-MAG3 is a standard radiotracer for renal dynamic functional study. Despite its"nproperties for clinical uses, it has numerous technical limitations. 99mTc-EC is also a tubular"nradiotracer for renal imaging, which has not been used worldwide. In this study, the use of 99mTc-EC"nand 99mTc-MAG3 for renal functional study were compared."nMethods: Thirty five patients (20 male, 15 female; mean age of 34.63 ±10.69 years) were entered in"nthe study. About 1...

  9. Sustained availability of 99mTc: possible paths forward.

    Science.gov (United States)

    Pillai, Maroor Raghavan Ambikalmajan; Dash, Ashutosh; Knapp, F F Russ

    2013-02-01

    The availability of (99m)Tc for single-photon imaging in diagnostic nuclear medicine is crucial, and current availability is based on the (99)Mo/(99m)Tc generator fabricated from fission-based molybdenum (F (99)Mo) produced using high enriched uranium (HEU) targets. Because of risks related to nuclear material proliferation, the use of HEU targets is being phased out and alternative strategies for production of both (99)Mo and (99m)Tc are being evaluated intensely. There are evidently no plans for replacement of the limited number of reactors that have primarily provided most of the (99)Mo. The uninterrupted, dependable availability of (99m)Tc is a crucial issue. For these reasons, new options being pursued include both reactor- and accelerator-based strategies to sustain the continued availability of (99m)Tc without the use of HEU. In this paper, the scientific and economic issues for transitioning from HEU to non-HEU are also discussed. In addition, the comparative advantages, disadvantages, technical challenges, present status, future prospects, security concerns, economic viability, and regulatory obstacles are reviewed. The international actions in progress toward evolving possible alternative strategies to produce (99)Mo or (99m)Tc are analyzed as well. The breadth of technologies and new strategies under development to provide (99)Mo and (99m)Tc reflects both the broad interest in and the importance of the pivotal role of (99m)Tc in diagnostic nuclear medicine.

  10. Quality control of techetium 99m radiopharmacentical in nuclear medicine

    International Nuclear Information System (INIS)

    Gel chromatography column scanning (GCS) is a new method for radiochemical quality control. GCS techniques for Technetium-99m radiopharmaceuticals in nuclear medicine have been developed for use in both research and routine clinical work. The dependence on several of the parameters of the GCS method have been investigated, e.g. type of gel, column dimensions, eluent, equilibration, elution volume, flow rate and resolution of the recording system (radiochromatographic scanner or scintillation camera). The GCS method has been compared with conventional gel filtration, thinlayer cromatography (TLC) and paper cromatography (PC). The GCS method is to be preferred due to few artifacts, much information, good reproducibility, rapidity, simplicity and the convenience of the test. The GCS method has been applied to the development of labelling techniques for the new radiopharmaceuticals Tc-99m plasmin and Tc-99m unithiol (2.3 dimercaptopropane sodiumsulphonate), use for investigating deep vein thrombosis and renal cortical morphology respectively. The GCS method has also been applied for studying some labelling parameters, the radiochemical purity and the labelling of Tc-99m macroaggregated albumin, Tc-99m pyrophosphate, Tc-99m methylenedisphosphate, in addition to Tc-99m plasmin and Tc-99m unithiol. (Author)

  11. Muscle localization of Tc-99m MDP after exertion

    Energy Technology Data Exchange (ETDEWEB)

    Valk, P.

    1984-09-01

    Very high muscle uptake of Tc-99m MDP was seen two days after the start of a program of vigorous weight-lifting exercises. Localization of Tc-99m bone tracer in muscle that has been damaged by exertion may be a more common phenomenon than is recognized at present.

  12. Initial experience with 99mTc-tetrofosmin scintimammography

    International Nuclear Information System (INIS)

    Full text:99mTc-tetrofosmin prone scintimammography was performed in 12 patients with palpable breast lesions and abnormal mammography findings. Right and left lateral prone and anterior supine views were obtained starting at 10 min of intravenous injection of 20 mCi 99mTc labelled tetrofosmin. Focal increased concentration of radiopharmaceutical as compared to surrounding normal breast tissue was considered positive for malignant disease. Patients underwent surgery with histopathological examination. 7 of the eight malignant lesions showed accumulation of 99mTc-tetrofosmin. Involved lymph nodes were also seen to concentrate the radiopharmaceutical. Four benign breast masses did not show focal 99mTc-tetrofosmin uptake. Initial results suggest that 99mTc-tetrofosmin could play a useful role in evaluation of breast tumours

  13. Comparative scintigraphic study of renal function with 99mTc-Mercaptoacetyl-triglycine and 99mTc-Ethylenedicysteine

    Directory of Open Access Journals (Sweden)

    Beiki D.

    2008-03-01

    Full Text Available 99mTc-MAG3 is a standard radiotracer for renal dynamic functional study. Despite its"nproperties for clinical uses, it has numerous technical limitations. 99mTc-EC is also a tubular"nradiotracer for renal imaging, which has not been used worldwide. In this study, the use of 99mTc-EC"nand 99mTc-MAG3 for renal functional study were compared."nMethods: Thirty five patients (20 male, 15 female; mean age of 34.63 ±10.69 years were entered in"nthe study. About 10 mCi of 99mTc-EC and 99mTc-MAG3 were administered in different days within 7"ndays intervals and serial images were obtained for 30 minutes. Serum creatinine and visual"nscintigraphic findings of all patients were within normal limits."nResults: In this study, the renal uptake of 99mTc-EC was significantly higher than 99mTc-MAG3 (6.20%"nvs 4.39% of the injected dose, while the hepatic activity of 99mTc-EC was significantly lower (307 vs"n439 mean pixel count, p<0.0001. Also the absolute values for some other quantitative parameters"nsuch as right and left kidney transit times and ERPF were different for each radiotracer. In spite of"nthese differences most quantitative parameters (except for right kidney transit time, liver uptake and"nTmax of both kidneys showed good correlations for both agents."nConclusion: The calculated ERPF with 99mTc-EC as compared to 99mTc-MAG3 is in closer proximity"nto the true values. Also the use of 99mTc-EC compared to 99mTc-MAG3 in clinic due to simplicity of"npreparation, higher stability, higher renal uptake, and lower hepatobiliary activity is better. Therefore"nthe use of 99mTc-EC as an appropriate substitute or ever preferred radiopharmaceutical for 99mTc-"nMAG3 radiopharmaceutical for renal function studies is recommended.

  14. Evaluation of the absorbed dose to the kidneys due to Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3) and Tc{sup 99m} (Dmsa); Evaluacion de la dosis absorbida en los rinones debido al Tc{sup 99m} (DTPA) / Tc{sup 99m} (MAG3) y Tc{sup 99m} (DMSA)

    Energy Technology Data Exchange (ETDEWEB)

    Vasquez A, M.; Murillo C, F.; Castillo D, C.; Rocha J, J.; Sifuentes D, Y.; Sanchez S, P. [Universidad Nacional de Trujillo, Av. Juan Pablo II s/n, Trujillo (Peru); Idrogo C, J.; Marquez P, F., E-mail: marvva@hotmail.com [Instituto Nacional de Enfermedades Neoplasicas, Av. Angamos 2520, Lima (Peru)

    2015-10-15

    The absorbed dose in the kidneys of adult patients has been assessed using the biokinetics of radiopharmaceuticals containing Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3) or Tc{sup 99m} (Dmsa).The absorbed dose was calculated using the formalism MIRD and the Cristy-Eckerman representation for the kidneys. The absorbed dose to the kidneys due to Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3), are given by 0.00466 mGy.MBq{sup -1} / 0.00339 mGy.MBq{sup -1}. Approximately 21.2% of the absorbed dose is due to the bladder (content) and the remaining tissue, included in biokinetics of Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3). The absorbed dose to the kidneys due to Tc{sup 99m} (Dmsa) is 0.17881 mGy.MBq{sup -1}. Here, 1.7% of the absorbed dose is due to the bladder, spleen, liver and the remaining tissue, included in biokinetics of Tc{sup 99m} (Dmsa). (Author)

  15. Evaluation of adenosine preconditioning with 99mTc-His10-annexin V in a porcine model of myocardium ischemia and reperfusion injury: preliminary study

    International Nuclear Information System (INIS)

    Purpose: The goal of this study was to evaluate the feasibility of 99mTc-His10-annexin V for the detection of acute myocardial cell death and to assess the effect of adenosine preconditioning in a porcine model of myocardium ischemia and reperfusion injury (RI). Materials and Methods: 99mTc-His10-annexin V was prepared by one-step direct labeling, and RCP and radiostability were tested. The binding of 99mTc-His10-annexin V to apoptosis was validated in vitro using camptothecin-induced Jurkat cells. In vivo biodistribution was determined in mice by the dissection method. Ischemia of 20-30 min was induced by balloon occlusion of the epicardial coronary artery of the porcine model (n=14). Adenosine was infused intravenously in six pigs before coronary occlusion. 99mTc-His10-annexin V (n=12) was injected intravenously at 1 h after reperfusion. SPECT/CT was acquired at 3 h postinjection. Myocardial perfusion imaging (MPI) with 99mTc-MIBI was also performed 1 day after His10-annexin V imaging. Cardiac tissues were analyzed postmortem using hematoxylin-and-eosin and TUNEL staining. Caspase-3 activity was measured to confirm the presence of apoptosis. Results: 99mTc-His10-annexin V had a RCP >98% and high stability 2 h after radiolabeling; it could bind to apoptotic cells with high affinity. Biodistribution of 99mTc-His10-annexin V showed a predominant uptake in the kidney and relatively low uptake in the myocardium, liver and gastrointestinal tract; rapid clearance from blood and kidney was observed. In the untreated group, intense uptake of His10-annexin V was visualized in the defect which was shown in MPI, whereas in the adenosine group a mild uptake of 99mTc-His10-annexin was found in the risk area which showed no defects in the 99mTc-MIBI image. TUNEL staining and activated caspase-3 confirmed the ongoing apoptosis in RI. Adenosine preconditioning significantly diminished the level of apoptosis. Uptake of His10-annexin V in RI correlated with TUNEL-positive nuclei

  16. Scintigraphic and intraoperative scintimetric localization of an ectopic parathyroid adenoma with Tc-99m Tetrofosmin and Tc-99m Sestamibi

    International Nuclear Information System (INIS)

    Background: We report on a case of preoperative scintigraphic and intraoperative scintimetric confirmation of an ectopic parathyroid adenoma with Tc-99m Tetrofosmin in a 52-year old patient with persistent hyperparathyroidism after unsuccessful primary preoperative localization and reoperation. Methods: In preoperative localization study, dynamic, sequential static scans and SPECT of the neck and mediastinum were performed using Tc-99m Sestamibi and Tc-99m Tetrofosmin. Reoperation was performed by help of a hand-held gamma-probe for intraoperative scintimetric localization after preoperative injection of Tc-99m Tetrofosmin. Results: An ectopic adenoma could be detected preoperatively with both tracers. Tc-99m Tetrofosmin showed no significant difference in image quality vs. Tc-99m Sestamibi. The adenoma could also be detected scintimetrically by elevated count-rates. Conclusions: This case gives promise for Tc-99m Tetrofosmin to be a possible alternative in parathyroid imaging with similar imaging properties and slight theoretical advantages concerning background clearance, radiation exposure and constitution over Tc-99m Sestamibi. Intraoperative localization and confirmation of ectopic parathyroid adenoma, eventually with a geometrically adapted gamma-probe, seems to be possible. Whether there are significant advantages resulting in a possible reduction of operating time, should be evaluated in further studies. (author)

  17. Tc-99m Ciprofloxacin SPECT of Pulmonary Tuberculosis

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Min Kyung; Hwang, Kyung Hoon [Gachon University Gil Hospital, Incheon (Korea, Republic of); Yoon, Min Ki [Good Samaritan Hospital, Pohang (Korea, Republic of); Choe, Won Sick [Kangbuk Samsung Hospital, Seoul (Korea, Republic of)

    2010-06-15

    Tc-99m ciprofloxacin is available for imaging infection. However, there has been no study on employing single photon emission computed tomography (SPECT) with using Tc-99m ciprofloxacin to image active pulmonary tuberculosis. Therefore, we conducted this study to assess the efficacy of Tc-99m ciprofloxacin SPECT for imaging active pulmonary tuberculosis. Twenty-one participants were enrolled in this prospective study. They were divided into two groups according to the clinical and radiological assessment. Group one (Gr. 1) consisted of five normal volunteers and six patients with inactive pulmonary tuberculosis. Group two (Gr. 2) consisted of ten patients with active pulmonary tuberculosis. SPECT was performed 3 h after injecting 555 MBq (15 mCi) of Tc-99m ciprofloxacin. The findings of Tc-99m ciprofloxacin SPECT were interpreted by a nuclear medicine specialist and then the results were analyzed according to the patients' clinical and radiological classifications. The results of Tc-99m ciprofloxacin SPECT were as follows: eight true-positive cases, ten true-negative cases, one false-positive case and two false-negative cases. The sensitivity and specificity was 80.0% and 90.0%, respectively. The positive predictive value was 88.9% and the negative predictive value was 83.3%. Conclusions Tc-99m ciprofloxacin SPECT is feasible for imaging active pulmonary tuberculosis. It is a useful nuclear-imaging method for discriminating between the active and inactive tuberculosis states in patients with a past medical history of pulmonary tuberculosis.

  18. Tc-99m Ciprofloxacin SPECT of Pulmonary Tuberculosis

    International Nuclear Information System (INIS)

    Tc-99m ciprofloxacin is available for imaging infection. However, there has been no study on employing single photon emission computed tomography (SPECT) with using Tc-99m ciprofloxacin to image active pulmonary tuberculosis. Therefore, we conducted this study to assess the efficacy of Tc-99m ciprofloxacin SPECT for imaging active pulmonary tuberculosis. Twenty-one participants were enrolled in this prospective study. They were divided into two groups according to the clinical and radiological assessment. Group one (Gr. 1) consisted of five normal volunteers and six patients with inactive pulmonary tuberculosis. Group two (Gr. 2) consisted of ten patients with active pulmonary tuberculosis. SPECT was performed 3 h after injecting 555 MBq (15 mCi) of Tc-99m ciprofloxacin. The findings of Tc-99m ciprofloxacin SPECT were interpreted by a nuclear medicine specialist and then the results were analyzed according to the patients' clinical and radiological classifications. The results of Tc-99m ciprofloxacin SPECT were as follows: eight true-positive cases, ten true-negative cases, one false-positive case and two false-negative cases. The sensitivity and specificity was 80.0% and 90.0%, respectively. The positive predictive value was 88.9% and the negative predictive value was 83.3%. Conclusions Tc-99m ciprofloxacin SPECT is feasible for imaging active pulmonary tuberculosis. It is a useful nuclear-imaging method for discriminating between the active and inactive tuberculosis states in patients with a past medical history of pulmonary tuberculosis.

  19. New preparation method for /sup 99m/Tc-phytate

    International Nuclear Information System (INIS)

    A new method is described in which Pt-Sn or Sn-Sn electrodes are used to activate /sup 99m/TcO4-. The /sup 99m/Tc is incorporated into phytic acid by stannous ion released from a tin anode by the corrosive reaction. The most suitable pH for labeling phytic acid by this method was below 5 and the /sup 99m/Tc-phytate formed could be precipitated with Ca+2 at a pH above 3.5. Though /sup 99m/Tc-phytate is soluble in aqueous solution, it forms an insoluble species with Ca+2 in vivo and is trapped in the reticuloendothelial system. More than 93 percent of the /sup 99m/Tc-phytate localized in the liver of mice; here its biologic half-time is about 112 hr. The /sup 99m/Tc-phytate prepared by this method promises to be useful as a liver-scanning agent. Since our method does not require an applied electric potential, it appears to be one of the most convenient methods for labeling compounds with /sup 99m/Tc

  20. {sup 99m}Tc-sestamibi. Clinical applications

    Energy Technology Data Exchange (ETDEWEB)

    Bucerius, Jan [Maastricht Univ. Medical Center (Netherlands). Dept. of Nuclear Medicine and Cardiovascular Research Institute Maastricht; Ahmadzadehfar, Hojjat; Biersack, Hans-Juergen (eds.) [Universitaetsklinikum Bonn (Germany). Klinik und Poliklinik fuer Nuklearmedizin

    2012-07-01

    {sup 99m}Tc-sestamibi is a single-photon emission computed tomography (SPECT) radiotracer that was introduced into clinical routine for myocardial perfusion imaging more than two decades ago. Although today the main application of {sup 99m}Tc-sestamibi SPECT remains the imaging of myocardial perfusion, it is also an accepted and well-proven imaging technique for a variety of oncologic and non-oncologic applications, including brain, breast, and thyroid cancer and thyroid and parathyroid adenoma. Its efficacy in a range of indications ensures that {sup 99m}Tc-sestamibi SPECT will remain widely used despite the rapid diffusion of 18F-FDG PET. {sup 99m}Tc-Sestamibi - Clinical Applications provides a detailed and informative overview of almost all the oncologic and non-oncologic applications of {sup 99m}Tc-sestamibi SPECT, including several relatively rare indications. Different disease-related protocols for {sup 99m}Tc-sestamibi SPECT are presented, and for each disease a comprehensive summary of the relevant pathology and epidemiology is provided. Throughout, there is a strong emphasis on the practical aspects of use of this popular tracer, including instructions for the preparation of several commercially available tracer kits. Clinical practitioners will find this book to be an invaluable guide to the application and benefits of {sup 99m}Tc-sestamibi SPECT in both the inpatient and the outpatient setting. (orig.)

  1. QC studies and biological evaluations of three locally produced renal pharmaceutical preparations: 99mTc-DTPA, 99mTc-GH, and 99mTc- DMSA

    International Nuclear Information System (INIS)

    Quality control (QC) studies were done of three locally produced renal preparations of 99mTc- DTPA, 99mTc-GH and 99mTc-DMSA, as part of the production activity before the Gulf war. These studies were performed using gel chromatography column scanning (GCS) technique, although there are other alternative techniques. These kits were tested for radiochemical purity, and the labeling yields were found to be excellent. Biological studies included the following parameters: organ distribution (in mice, rats and rabbits), blood clearance in rabbits, and plasma protein binding in rats at different time intervals. Poor renal concentration of three agents in mice was significant at two time intervals. The tissue distribution in mice of DTPA, GH, and DMSA kits, which were stored at 37 deg. C for 30 days, after intravenous injection of the radioactivity at 5, 30, and 60 minutes, respectively, is presented. Blood retention of the renal agents was considerably low compared to those in rats and rabbits at the respective time intervals. The blood clearance of 99mTc-DMSA was relatively slow, and 99mTc-GH has rapid blood clearance, identical with that of 99mTc-DTPA for the first 20 minutes but slower thereafter. The binding of DTPA, GH and DMSA with plasma protein was 5, 65, 95 % at 1 h, respectively. Various amounts of tin (II) (as SnCl2), were used in the kits, with up to 10 times more of the usual dose for respective tin-complex. It was observed that there was no significant variation of the organ distribution in mice

  2. Study of 99mTc-ECD instant kit

    International Nuclear Information System (INIS)

    The authors report the preparation of 99mTc-ECD instant kit, and a comparison of the instant kit with the ligand exchange kit. The radiochemical purity, stability, animal experiment and preliminary clinical trial showed that the instant kit possessed the following advantages: (1) The labelling yield (greater than 95%) and stability of 99mTc-ECD (the liberation of 99mTc 5 hr after preparation was less than 1%) were high; (2) The labelling method was simple, convenient and effective; (3) The quality of brain SPECT images was good. This instant kit is more suitable for the clinic application than the 2-step kit

  3. {sup 99m}Tc-tetrofosmin or {sup 99m}Tc-sestamibi for double-phase parathyroid scintigraphy?

    Energy Technology Data Exchange (ETDEWEB)

    Froeberg, Alida C.; Valkema, Roelf [Department of Nuclear Medicine, Erasmus MC, Dr. Molewaterplein 40, 3015 GD Rotterdam (Netherlands); Bonjer, Jaap H. [Department of Surgery, Erasmus MC, Rotterdam (Netherlands); Krenning, Eric P. [Department of Nuclear Medicine, Erasmus MC, Dr. Molewaterplein 40, 3015 GD Rotterdam (Netherlands); Department of Internal Medicine, Erasmus MC, Rotterdam (Netherlands)

    2003-02-01

    Several years ago technetium-99m tetrofosmin was reported to localise parathyroid adenomas. The aim of this study was to compare the sensitivity of this radiopharmaceutical with that of {sup 99m}Tc-sestamibi using a double-phase parathyroid scintigraphy protocol. Scans of 12 patients were evaluated visually and lesion to thyroid ratios were calculated. Nine of the patients were subsequently operated on; a total of eight parathyroid adenomas or hyperplastic glands were histologically confirmed in seven of the patients, while in one patient a parathyroid carcinoma was histologically proven. All of these patients had positive {sup 99m}Tc-sestamibi scintigrams, whereas only two {sup 99m}Tc-tetrofosmin scintigrams were positive. With {sup 99m}Tc-sestamibi there was a significant increase in the lesion to thyroid ratio from 10 min to 90 min and 150 min p.i. which was not seen on scintigraphy with {sup 99m}Tc-tetrofosmin. This makes {sup 99m}Tc-tetrofosmin less suitable for double-phase parathyroid scintigraphy. (orig.)

  4. [Diagnosis of achalasia using 99m-Tc pertechnetate scintigraphy].

    Science.gov (United States)

    Mikosch, P; Gallowitsch, H J; Kresnik, E; Lind, P

    1997-06-01

    A 73-year-old patient presented a 99mTc scintiscan with a focal tracer accumulation left and caudal of the thyroid gland. Further investigations with sonography, CT, esophagoscopy and barium swallow provided the diagnosis of achalasia as the reason for focal 99mTc retention caudal of the thyroid gland. Explanation for 99mTc accumulation within the esophagus may be the nonspecific accumulation of 99mTc not only in the thyroid gland but also in the salivary glands. Excretion of the tracer follows with the saliva. Structural and motor disorders of the esophagus can thus lead to focal tracer retention within the esophagus simulating thyroid tissue. PMID:9395425

  5. Preparation of 99mTc-HYNIC-Annexin V

    Institute of Scientific and Technical Information of China (English)

    LAN Xiao-Li; ZHANG Yong-Xue

    2005-01-01

    99mTc-HYNIC-Annexin V was prepared for apoptosis radionuclide imaging in vivo. The expression plasmid of human Annexin V was constructed and expressed in E. coli. HYNIC was prepared and used as bifunctional chelating agent for 99mTc labeling. The radiochemical purity, radiolabeling yield and stability of 99mTc-HYNIC-Annexin V were studied. The coding sequence of human Annexin V gene was successfully cloned into the expression vector, and highly expressed in E. coli. A protein with a molecular weight of about 36kD could be induced and confirmed by Western Blotting and SDS-PAGE. Annexin V could be labeled with 99mTc using HYNIC, and showed high radiochemical purity of more than 90%. The radiolabeling yield could reach more than 90%. The reaction condition was moderate. It suggested that 99nTc-HYNIC-Annexin V may be a promising agent for apoptosis imaging and clinical application.

  6. Discordant results in Tc-99m tetrofosmin and Tc-99m sestamibi parathyroid scintigraphies; Resultados discordantes em cintilografias das paratireoides realizadas com tetrofosmin-99mTc e com sestamibi-99mTc

    Energy Technology Data Exchange (ETDEWEB)

    Duarte, Paulo Schiavom; Domingues, Fernanda C.; Santi Costa, Michele; Brandao, Cynthia; Oliveira, Marco A.C. de; Vieira, Jose G.H. [Fleury - Centro de Medicina Diagnostica, Sao Paulo, SP (Brazil)]. E-mail: paulo.duarte@fleury.com.br

    2007-10-15

    Parathyroid scintigraphies have been used to detect pathological parathyroid glands either before as well as after the parathyroid resection surgery in patients with hyperparathyroidism. One of the most utilized techniques to perform the studies is the double-phase images with Tc-99m sestamibi, which has been shown to be very accurate in the localization of enlarged parathyroid glands. Similar to Tc-99m sestamibi, Tc-99m tetrofosmin is a radiopharmaceutical initially developed to perform myocardial perfusion study that has been used to perform parathyroid scintigraphies. Although most of the papers suggest that the overall sensitivities of both radiopharmaceuticals are similar, there are some papers questioning the accuracy of Tc-99m tetrofosmin to detect abnormal parathyroid glands. In the present article, we report a case with discordant results by both methods. (author)

  7. The role of technetium-99m-labeled octreotide acetate scintigraphy in suspected breast cancer and correlates with expression of SSTR

    Energy Technology Data Exchange (ETDEWEB)

    Wang Feng [Department of Nuclear Medicine, Nanjing First Hospital affiliated to Nanjing Medical University, Nanjing 210006 (China)], E-mail: fengwangcn@hotmail.com; Wang Zizheng; Wu Jie; Qu Wei; Yao Weixuan [Department of Nuclear Medicine, Nanjing First Hospital affiliated to Nanjing Medical University, Nanjing 210006 (China); Zhao Jianhua [Department of Pathology, Nanjing First Hospital affiliated to Nanjing Medical University, Nanjing 210006 (China); Liu Zijun [Department of General Surgery, Nanjing First Hospital affiliated to Nanjing Medical University, Nanjing 210006 (China)

    2008-08-15

    Purpose: To evaluate the value of {sup 99m}Tc-octreotide acetate (hereafter, {sup 99m}Tc-octreotide) somatostatin receptor (SSTR) scintigraphy in the detection of primary breast cancer and to correlate with expression of SSTRs. Materials and Methods: Fifty-four female and 1 male patients (range, 17-77 years; mean age, 48 years) with palpable breast lesion were included in this study. {sup 99m}Tc-octreotide and {sup 99m}Tc-MIBI scintigraphy were undertaken in all patients, and the region of interest was drawn around each lesion. Tumor uptake was measured and expressed as the ratio of tumor to normal tissue activity (T/NT). Final clinical diagnosis was confirmed by histopathological analysis. Expression of SSTR1-5 mRNA was measured with RT-PCR in 15 patients with malignant neoplasm, and protein level of SSTR-2 and SSTR-5 was measured using immunohistochemical staining in 15 patients with malignant neoplasm and 18 patients with benign lesion. Results: Thirty-five patients were confirmed to have infiltrative ductal breast carcinoma, 1 patient with cellular cancer, 1 patient with adenocarcinoma and 18 patients had benign lesions. The sensitivity, specificity, positive predictive value and negative predictive value of {sup 99m}Tc-octreotide in detection of primary lesion were 91.8%, 22.2%, 71.8% and 57.1%, respectively. No significant correlation was observed between T/NT of {sup 99m}Tc-octreotide and {sup 99m}Tc-MIBI. SSTR mRNA subtypes were variably expressed. SSTR3 was the most highly expressed, followed by SSTR1, SSTR2 and SSTR5; SSTR4 was the least expressed in the level of mRNA of SSTR. Significant correlation was shown between T/NT and the expression of SSTR2 mRNA (r=0.73, P<.01). Conclusion: {sup 99m}Tc-octreotide acetate scintigraphy was sensitive for the detection of primary lesion of breast cancer; however, nonspecific breast tissue uptake hampered the specificity and clinical value in the detection of lymph node metastasis. Five subtypes of SSTR mRNA and

  8. Labelling of biological structures with technetium 99 m

    International Nuclear Information System (INIS)

    The labelling of red blood cells (RBC) with technetium 99m (99m Tc) depends on several factors, as the stannous ion (Sn++) concentration, the time and temperature of incubation, the anticoagulant utilized, the presence of plasma proteins (PP) and others. Although the blinding of 99m Tc with hemoglobin and PP are similar, they appear to have specific characteristics as demonstrated by precipitation with alcohol, acetone, trichloroacetic acid, hydrochloric acid and mercury chloride. The bacterial cultures labeled with Technetium-99m, at optimal Sn++ ion concentration, presents a large stability and their viability is not altered by this treatment. The electrophoretic mobility, the hydrophobicity, the cationized ferritin distribution and the adherence to human buccal epithelial cells are not modified either. The possibility of labelling with 99m Tc of planaria and cercariae of Schistossoma mansoni evaluative cycle increases the utilization of this radionuclide to an experimental level. The results described with the labelling of these biological structures with 99m Tc demonstrated that stable labeled and viable operations are obtained. (author)

  9. Tumour imaging using technetium-99m-citrate

    International Nuclear Information System (INIS)

    Sixteen patients with soft tissue malignancy or fibroadenoma of the breast (Group A) were imaged using 99mTc-citrate. Majority of the patients (n=14) has new untreated lesions. Appreciable skeletal uptake of the tracer was serendipitously noticed in all cases. One of these had widespread bone metastases seen almost identically in 99mTc-citrate and 99mTc-MDP studies. Accordingly, 10 patients (Group B) having more than 40 malignant lesions on the bone scan underwent 99mTc-citrate study. In group A, accumulation of the tracer was seen in all malignant breast nodules and axillary lymphnode mass (n=4), medullary carcinoma of the thyroid along with its metastasis and a carcinoid (n=4) and an ovarian malignancy. Uptake and outflow pattern could differentiate fibroadenoma (n=3) from carcinoma of the breast. No significant uptake was seen in liver secondaries (n>10), lymphoma lesions (n=5), papillary carcinoma of thyroid, renal cell and embryonal cell carcinoma. In group B patients, the radiotracer accumulated well in the metastatic lesions while there was distinctly lesser uptake in normal/degenerated joints compared to the bone scan. The study shows potential of the tracer in imaging soft tissue malignancies. Bone scanning with 99mTc-citrate is an interesting possibility since mechanism of its uptake appears to be different to 99mTc-MDP. (author)

  10. Technetium-99m pentavalent dimercaptosuccinic acid imaging for pituitary adenomas

    International Nuclear Information System (INIS)

    The authors performed scintigraphy using 99mTc(V)-DMSA (Technetium-99m pentavalent dimercaptosuccinic acid) on patients with pituitary adenomas. Three non-functioning (100%), 2 GH-secreting (67%), 4 PRL-secreting (80%), and zero ACTH-secreting (0%) adenomas concentrated the 99mTc(V)-DMSA, but all 5 of the non-adenomatous lesions and 1 normal pituitary gland did not. There was no significant relationship between tumor-to-background ratios and tumor sizes, or the serum hormone level. The 99mTc(V)-DMSA scintigraphy showed an overall sensitivity of 69% (9/13) in detecting pituitary adenomas, which increased to 82% for non-functioning, GH-secreting and PRL-secreting adenomas. In conclusions, 99mTc(V)-DMSA was found to be a suitable radiotracer for detecting pituitary adenomas. But further studies are necessary to define the processes that concentrate 99mTc(V)-DMSA and their role in pituitary adenomas. (author)

  11. Poor Tc-99m dimercaptosuccinic acid uptake, re-evaluation with Tc-99m MAG3 scintigraphy in Lowe syndrome

    OpenAIRE

    Koca, Gokhan; Atilgan, Hasan Ikbal; Demirel, Koray; Diri, Akif; KORKMAZ, Meliha

    2011-01-01

    Tc-99m dimercaptosuccinic acid (DMSA) is filtered through the glomeruli and reabsorbed by the proximal tubules as low molecular weight proteins. In Lowe syndrome this mechanism is impaired and so poor DMSA uptake is seen. Poor DMSA uptake was shown in very few studies, but none mentioned normal Tc-99m MAG3 uptake. In this case, the patient had poor DMSA uptake, normal MAG3 uptake and a neurogenic bladder in anterior to the left kidney that attenuates left kidney.

  12. {sup 99m}Tc-ECD and {sup 99m}Tc-HM-PAO SPECT in five patients with MELAS

    Energy Technology Data Exchange (ETDEWEB)

    Katagiri, Shinako; Nishimaki, Hiroshi; Kitano, Masashi; Horiike, Shigeharu; Kan, Shinichi; Ishii, Katsumi; Matsubayashi, Takashi; Sakai, Fumihiko [Kitasato Univ., Sagamihara, Kanagawa (Japan). School of Medicine

    1998-03-01

    Cerebral perfusion was studied in five patients with mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS), using single photon emission computed tomography (SPECT) with {sup 99m}Tc-ethyl cysteinate dimer ({sup 99m}Tc-ECD) or {sup 99m}Tc-hexamethyl propyleneamine oxime ({sup 99m}Tc-HM-PAO). In four cases, regional cerebral blood flow (rCBF) was evaluated by the method reported by Mastuda et al. Immediately after the stroke-like episodes, accumulation of the tracer was relatively increased in the temporooccipital lobe, and also increased rCBF was shown in the same area. However, the region showed decreased radioactivity at the chronic stage, and rCBF decreased also. These findings are consistent with positron emission tomography (PET) at the acute stage and autopsy. {sup 99m}Tc-ECD SPECT and {sup 99m}Tc-HM-PAO SPECT may be useful in the diagnosis and assessment of the progress of the MELAS. (author)

  13. Labeling cellular elements of blood with Technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Dewanjee, M.K.

    1990-08-01

    The purpose of this proposal is to develop new technique of labeling platelets and white cells with Tc-99m radionuclide. The conditions of labeling canine platelets and white cells with the lipid-soluble Tc-99m HMPAO have been optimized. The function of labeled platelets were evaluated by the determination of platelet survival time and recovery and these values were compared with that of In-111 tropolone labeled platelets. We developed the bilateral femoral catheterization model for the evaluation of platelet-thrombosis on control and heparin-bonded catheters in dogs. We are evaluating platelet thrombosis in the hollow-fiber hemodialyzer with Tc-99m and In-111 labeled platelets. We have developed the flow-loop for in vitro studies and are using a pig model for quantitation of platelet-consumption during hemodialysis. We are currently evaluating the new technique of platelet and white cell-labeling with Tc-99m and testing them in animal models of thrombosis and infection (osteo-myelitis). We are also using the Tc-99m HMPAO labeled mixed white cells in the early diagnosis (3-hour post-injection) of acute and chronic infection in patients and comparing the results with that of IN-111 oxine labeled white cells.

  14. Synthesis and formulation of 99m Tc-ECD radiopharmaceutical

    International Nuclear Information System (INIS)

    Nuclear medicine is a medical specialty which uses radioactive compounds (radionuclides) for diagnostic and therapeutic purposes. 99m Tc is the more common radionuclide used in many studies in nuclear medicine because its advantages: it has a photopeak of 140 KeV and a half-life of 6 hours; it can be eluted from a Molybdenum 99 generator, so radiopharmaceuticals can be prepared on site. Ethyl cysteine dimer (ECD) labelled with reduced Technetium 99m has been purposed recently as a promising radiopharmaceutical for brain perfusion imaging 99m Tc-ECD is a lipophilic neutral complex which cross the brain blood barrier and show high brain uptake. The objective of this work was synthesize and to design a freeze dried formulation for the instant preparation of 99m Tc-ECD complex useful for brain perfusion imaging. We obtained a freeze dried stable formulation for the preparation of 99m Tc-ECD kit with a radiochemical purity higher than 90 %, which fulfills with the quality control of radiopharmaceuticals. Furthermore, we developed analytic techniques for the determination of the different chemical compounds into the lyophilized kit. (Author)

  15. Determination of radiochemical yield of {sup 99m}Tc radiopharmaceutical preparations using gamma counter and linear radiochromatography scanner

    Energy Technology Data Exchange (ETDEWEB)

    Martins, Patricia de A.; Moura, Rebeca G.; Shiki, Andressa M.; Fukumori, Neuza T.O.; Matsuda, Margareth M.N., E-mail: patyosborne@yahoo.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    The radiochemical purity (RCP) evaluation is a prerequisite for radiopharmaceuticals before the administration in patients. RCP is defined as the proportion of the total radioactivity in the product that is present in the specified chemical form. The most widely used techniques for RCP determination in radiopharmaceutical preparations are thin layer chromatography (TLC-Al), instant thin layer chromatography (ITLC-SG) and paper chromatography (PC). These techniques combined with radioactivity detection are one of the most important tools in the RCP of the radiopharmaceutical compounds. Several methods are used for the determination of the spatial distribution of radioactivity on the strips. The aim of this study was to compare two methods for radioactivity measurement in the determination of RCP in {sup 99m}Tc radiopharmaceuticals using gamma counter and linear radiochromatography scanner. Lyophilized radiopharmaceuticals were labeled with {sup 99m}Tc. The analysis was carried out using TLC-Al and high performance thin layer chromatography (HPTLC-Cellulose) sheets, ITLC-SG and 3MM Whatman PC. The radioactivity distribution was determined by counting each strip during 1 minute in a radiochromatography TLC scanner. For comparison, the strips were cut into small pieces and each one was separately measured in a gamma-counter during 0.20 minutes in 70-210 KeV {sup 99m}Tc window. USP 36 and FDA specify that not less than 90% of the total radioactivity must be in the spot corresponding to {sup 99m}Tc labeled compound. In conclusion, the procedure for RCP determination of ALBUMINA-TEC, DEX500-TEC, ECD-TEC, MACRO-TEC and MIBI-TEC can be faster using radiochromatography. (author)

  16. Comparison of relative renal function measured with either 99mTc-DTPA or 99mTc-EC dynamic scintigraphies with that measured with 99mTc-DMSA static scintigraphy

    Directory of Open Access Journals (Sweden)

    F. C. Domingues

    2006-08-01

    Full Text Available OBJECTIVE: The aim of this study was to compare the renal function measured with either 99mTc-DTPA or 99mTc-EC dynamic scintigraphies with that measured using 99mTc-DMSA static scintigraphy. METHODS: the values of relative renal function measured in 111 renal dynamic scintigraphies performed either with 99mTc-DTPA (55 studies or with 99mTc-EC (56 studies were compared with the relative function measured using 99mTc-DMSA static scintigraphy performed within a 1-month period. The comparisons were performed using Wilcoxon signed rank test. The number of 99mTc-DTPA and 99mTc-EC studies that presented relative renal function different by more than 5% from that measured with 99mTc-DMSA, using chi square test were also compared. RESULTS: the relative renal function measured with 99mTc-EC is not statistically different from that measured with 99mTc-DMSA (p = 0.97. The relative renal function measured with 99mTc-DTPA was statistically different from that measured using 99mTc-DMSA, but with a borderline statistical significance (p = 0.05. The number of studies with relative renal function different by more than 5% from that measured with 99mTc-DMSA is higher for the 99mTc-DTPA scintigraphy (p = 0.04 than for 99mTc-EC. CONCLUSION: the relative renal function measured with 99mTc-EC dynamic scintigraphy is comparable with that measured with 99mTc-DMSA static scintigraphy, while the relative renal function measured with 99mTc-DTPA dynamic scintigraphy presents a significant statistical difference from that measured with 99mTc-DMSA static scintigraphy.

  17. 99mTc-Ciprofloxacin for diagnosis of bacterial infection

    International Nuclear Information System (INIS)

    Preparation of '9'9'mTc-Ciprofloxacin for diagnosis of bacterial infection was investigated in this research. Factors including pH, injected dose and the purity of 99mTc-Ciprofloxacin were determined. The optimal labeling condition of 99mTc-Ciprofloxacin was found to be at pH 4.0-5.0. In terms of efficiency, the injected dose per gram of infected area was 0.25-0.56, which lasted for the duration of 1-3 h after injection. The radiochemical purity remained >90% with stability for the duration of 6 h. Therefore, this study has demonstrated the preparation of sterile, pyrogen-free and non-toxic 99mTc-Ciprofloxacin.

  18. Tc-99m ciprofloxacin scintigraphy in evaluation of orthopedic infections

    International Nuclear Information System (INIS)

    Identification of bacterial infection in orthopedic practice is an important issue for appropriate management. Conventional radiography including CT examination has limited role in such a situation. A Tc-99m Ciprofloxacin has been developed to localize bacterial infection in order to differentiate infection from inflammation. Critical evaluation of role of Tc-99m Ciprofloxacin scintigraphy in orthopedic practice was done in the present study. 104 patients with suspected infection in orthopedic clinic were taken up for Tc-99m Ciprofloxacin scintigraphy. A dose of 370 MBq of Tc-99m Ciprofloxacin was injected intravenously. Imaging was performed using Siemens integrated DIACAM camera with low energy all parallel or high-resolution collimators. Multiple spot views at serial time (1 hour, 4 hours and 24 hours) were obtained in 256 x 256 matrix size in ICON computer. Focal area of abnormal radionuclide concentration, which increased with time, was considered to be the pattern suggestive of presence of infection. In cases of equivocal qualitative appearance, quantitation of uptake was ascertained by the count statistics obtained using region of interest program. Final diagnosis of sepsis was based on microbiological examination or evidence of beneficial response to antibiotic therapy. Tc-99 Ciprofloxacin scintigraphy showed most favourable results in peripheral tubercular as well as non-tubercular osteomyelitis, tubercular arthritis and in cases with prosthesis (including vascular stent). It showed limited role in Pott's spine. The best sensitivity, specificity and accuracy of Tc-99m Ciprofloxacin using the defined criteria was 90%, 84% and 88% respectively in cases with non-tubercular osteomyelitis. Serial imaging up to 24 hours was required in ∼ 70% of cases. In ∼ 30% of cases quantification was required to form an opinion. We conclude that Tc-99m Ciprofloxacin is a highly useful technique in the management of infection in orthopedic practice. The procedure should

  19. Transferrin receptor targeted imaging using the Tc-99m transferrin

    Energy Technology Data Exchange (ETDEWEB)

    Jung, H. J.; Kim, E. M.; Kim, B. C.; Kim, C. G [Wonkwang University Hospital, Iksan (Korea, Republic of)

    2004-07-01

    Transferrin receptors (Tf-R) were expressed on the surfaces of cancer cells. In cancer cells that are rapidly proliferating, transferrin receptors are upregulated due to the need for increasing non-hem iron to catalyze the first and rate-limiting step of DNA synthesis. Therefore transferrin (Tf) is thought to be a major ligand to find cancer cells. In this study, we investigated the targetability of Tf conjugated compounds to Tf-R expressed on cancer cells for detection and diagnosis and the usefulness of gamma probe-targeting delivery system on monitoring whether the gene complex bind to the cells specifically. For the detection and diagnosis of Tf-R positive cancer cells, Tf-chitosan conjugates were synthesized as previously described by Kircheis et al with some modifications. Succinimidyl 6-hydrazino nicotinate hydrochloride (HYNIC) was bound to Tf-chitosan conjugates. HYNlC-Tf-chitosan conjugates were labelled with 99m Tc. For tumor model, 5- to 6-week-old female BALB/c nude mice were injected subcutaneously in the left thigh with Ramos cells (human Burkitt's lymphoma). The gamma imagings were acquired after administration of Tc-99m Tf-chitosan conjugates. To compare the image acquired with HYNIC-Tf conjugate, Ga-67 study was performed. Radiolabeling yields of both Tf-chitosan conjugates was above 90% until 12hr. Uptake in the Ramos model of Tc-99m Tf-chitosan conjugate showed higher than those of Ga-67. A few minutes after injection Tc-99m Tf-chitosan conjugate localized mainly in the circulation (heart), kidneys, and tumor. At 24hr after injection, tumor to nontumor ratio of Ga-67 was approximately 4, but those of Tc-99m Tf-chitosan conjugate showed until 5 only 2 hr after injection. Using Tc-99m Tf-chitosan conjugate, transferrin binding to transferrin receptor on tumor cell could be seen in vivo.

  20. Technetium-99m sestamibi: an indicator of breast cancer invasiveness

    International Nuclear Information System (INIS)

    As recently shown, angiogenesis is the most reliable marker of breast cancer invasiveness. Unfortunately it must be assessed by immunohistochemistry on tissue specimens. We have used technetium-99m sestamibi, a marker of regional blood flow in other organs that often but not always images breast cancer, to assess the invasiveness of this tumour. Nineteen patients, ten with nodal metastases and nine without any metastases, were studied with 99mTc-sestamibi scintigraphy before operation. Angiogenesis was quantitatively assessed by immunohistochemical staining of endothelia for factor VIII. All the node-positive (N+) patients at surgical revesion showed a positive 99mTc-sestamibi scan of the primary tumour and all the N-patients were negative. Nine out of ten N+ and sestamibi-positive tumours showed more than 135 microvessels/mm2 and one showed 99 microvessels/mm2; by contrast there were 71.6±12.1 microvessels/mm2 in the nine N- and sestamibi-negative tumours. Our study suggests that 99mTc-sestamibi is a marker of breast cancer invasiveness: its uptake is related to angiogenesis and, possibly, to oxidative metabolism of the tumour. (orig.)

  1. Technetium-99m sestamibi: an indicator of breast cancer invasiveness

    Energy Technology Data Exchange (ETDEWEB)

    Scopinaro, F. (Section of Nuclear Medicine, Dept. of Experimental Medicine, Univ. ' La Sapienza' , Rome (Italy)); Schillaci, O. (Section of Nuclear Medicine, Dept. of Experimental Medicine, Univ. ' La Sapienza' , Rome (Italy)); Scarpini, M. (1st Inst. of Surgery, Univ. ' La Sapienza' , Rome (Italy)); Mingazzini, P.L. (1st Inst. of Surgery, Univ. ' La Sapienza' , Rome (Italy)); Di Macio, L. (Section of Nuclear Medicine, Dept. of Experimental Medicine, Univ. ' La Sapienza' , Rome (Italy)); Banci, M. (Section of Nuclear Medicine, Dept. of Experimental Medicine, Univ. ' La Sapienza' , Rome (Italy)); Danieli, R. (Section of Nuclear Medicine, Dept. of Experimental Medicine, Univ. ' La Sapienza' , Rome (Italy)); Zerilli, M. (1st Inst. of Surgery, Univ. ' La Sapienza' , Rome (Italy)); Limiti, M.R. (1st Inst. of Surgery, Univ. ' La Sapienza' , Rome (Italy)); Centi Colella, A. (Section of Nuclear Medicine, Dept. of Experimental Medicine, Univ. ' La Sapienza' , Rome (Italy))

    1994-09-01

    As recently shown, angiogenesis is the most reliable marker of breast cancer invasiveness. Unfortunately it must be assessed by immunohistochemistry on tissue specimens. We have used technetium-99m sestamibi, a marker of regional blood flow in other organs that often but not always images breast cancer, to assess the invasiveness of this tumour. Nineteen patients, ten with nodal metastases and nine without any metastases, were studied with [sup 99m]Tc-sestamibi scintigraphy before operation. Angiogenesis was quantitatively assessed by immunohistochemical staining of endothelia for factor VIII. All the node-positive (N+) patients at surgical revesion showed a positive [sup 99m]Tc-sestamibi scan of the primary tumour and all the N-patients were negative. Nine out of ten N+ and sestamibi-positive tumours showed more than 135 microvessels/mm[sup 2] and one showed 99 microvessels/mm[sup 2]; by contrast there were 71.6[+-]12.1 microvessels/mm[sup 2] in the nine N- and sestamibi-negative tumours. Our study suggests that [sup 99m]Tc-sestamibi is a marker of breast cancer invasiveness: its uptake is related to angiogenesis and, possibly, to oxidative metabolism of the tumour. (orig.)

  2. Technetium-99m Radiopharmaceuticals in Neurology. Chapter 6

    International Nuclear Information System (INIS)

    The ideal radioisotope for single photon emission computed tomography imaging is 99mTc, due to its physical decay characteristics, its availability through commercially available generator systems and its low cost per dose. Technetium-99m hydrophilic complexes are used to evaluate the integrity of the blood-brain barrier, while neutral and lipophilic complexes are used as brain perfusion imaging agents for determination of changes in regional cerebral blood flow in various neurological disorders. Radiopharmaceuticals that bind to central nervous system (CNS) receptors in vivo are useful for understanding the pathophysiology of a number of neurological and psychiatric disorders, their diagnosis and treatment. Nowadays, CNS receptor imaging agents are, with some exceptions, typically positron emission tomography radionuclide based radiopharmaceuticals. The reason for this is not based on principal but is rather as a result of the fact that efforts in the direction of 99mTc containing agents have not been strong or consistent enough. In the chapter, the progress made in the development of 99mTc complexes for imaging dopamine transporter, 5-HT1A receptor and amyloid plaques is presented. (author)

  3. Osteomyelitis diagnosis by {sup 99m}Tc radiolabeled aptamers

    Energy Technology Data Exchange (ETDEWEB)

    Santos, S.R.; Ferreira, I.M.; Andrade, A.S.R., E-mail: sararoberta7@hotmail.com, E-mail: imendesf@yahoo.com.br, E-mail: antero@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Barros, A.L.B.; Cardoso, V.N.; Diniz, O.F., E-mail: brancodebarros@yahoo.com.br, E-mail: valbertcardoso@yahoo.com.br, E-mail: simoneodilia@yahoo.com.br [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Faculdade de Farmacia. Departamento de Analises Clinicas e Toxicologicas

    2015-07-01

    Osteomyelitis, which is characterized by progressive inflammatory destruction and new opposition of bone, is still a difficult infection to treat. The clinical diagnosis in late stages is achieved easily, but an early diagnosis is more challenging. Staphylococcus aureus is a common agent found in osteomyelitis and bone prostheses infection. Diagnosis by scintigraphy has advantages because it is a non-invasive procedure and is able to perform an early diagnosis even before anatomic changes. Thus, nuclear medicine could contribute to an accurate diagnosis since specific radiopharmaceuticals were developed. In this study, aptamers selected to Staphylococcus aureus were labeled with {sup 99m}Tc and used for bacteria identification in an osteomyelitis experimental model. The aptamers selected to S. aureus were directly labelled with {sup 99m}Tc and were evaluated by biodistribution studies. Wistar rats with intraosseous infection in the right paw were used. A random aptamer labelled with {sup 99m}Tc was as control. Six animals were used in each group. The aptamers labeled with {sup 99m}Tc were able to identify the infection foci caused by S. aureus displaying a target/non-target ratio of 2,23 ± 0,20, after 3 h. The control group presented a target/non-target ratio 1,08 ± 0.23. The results indicated that the radiolabeled aptamers were able to identify specifically the infection foci and they should be further explored for infection diagnosis by scintigraphy. (author)

  4. Preparation and quality control of fibrinogen 99mTc

    International Nuclear Information System (INIS)

    A method of fibrinogen preparation is presented in order to label it with 99mTc employing Sn as reducing agent in alkaline medium. Purity controls by chromatography, coagulation in rabbits and biodistribution in rats were performed. It is concluded that optimal time incubation is between 22 and 23 hs. (M.E.L.)

  5. 99m Tc-labeled heparin test in orthopaedic surgery

    Energy Technology Data Exchange (ETDEWEB)

    Bouvier, J.F.; Lafon, J.C.; Colin, M.; Chatelut, J.; Beaubatie, F. (Hopital Universitaire Dupuytren, Limoges (France))

    1983-06-30

    99m Tc-labeled heparin test was performed for early detection of phlebitis or pulmonary embolism after orthopaedic prothesis. Heparinic treatment and surgery per se were demonstrated to have no effect on the results. If this test demonstrates a statistical difference for pathologic patients, it is of greater value to consider ratio between rates before and after intervention.

  6. Osteomyelitis diagnosis by 99mTc radiolabeled aptamers

    International Nuclear Information System (INIS)

    Osteomyelitis, which is characterized by progressive inflammatory destruction and new opposition of bone, is still a difficult infection to treat. The clinical diagnosis in late stages is achieved easily, but an early diagnosis is more challenging. Staphylococcus aureus is a common agent found in osteomyelitis and bone prostheses infection. Diagnosis by scintigraphy has advantages because it is a non-invasive procedure and is able to perform an early diagnosis even before anatomic changes. Thus, nuclear medicine could contribute to an accurate diagnosis since specific radiopharmaceuticals were developed. In this study, aptamers selected to Staphylococcus aureus were labeled with 99mTc and used for bacteria identification in an osteomyelitis experimental model. The aptamers selected to S. aureus were directly labelled with 99mTc and were evaluated by biodistribution studies. Wistar rats with intraosseous infection in the right paw were used. A random aptamer labelled with 99mTc was as control. Six animals were used in each group. The aptamers labeled with 99mTc were able to identify the infection foci caused by S. aureus displaying a target/non-target ratio of 2,23 ± 0,20, after 3 h. The control group presented a target/non-target ratio 1,08 ± 0.23. The results indicated that the radiolabeled aptamers were able to identify specifically the infection foci and they should be further explored for infection diagnosis by scintigraphy. (author)

  7. Revision: quality control of {sup 99m}Tc; Revisao: controle da qualidade do {sup 99m}Tc

    Energy Technology Data Exchange (ETDEWEB)

    Alves, S.M.; Kabad, M.C. [Universidade Estacio de Sa (UESA), Rio de Janeiro, RJ (Brazil); Teixeira, G.J.; Sousa, C H.S., E-mail: sanny.alves@yahoo.com.br [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

    2015-07-01

    The radiopharmaceutical most commonly used in nuclear medicine is technetium-99m. Radiopharmaceuticals labeled with {sup 99m}Tc are the main agents for diagnosis, being used in the vast majority of procedures due to radionuclide characteristics. During its preparation impurities can be generated that make products with different qualities of the desired. In this paper were presented some of the factors that can interfere with radiopharmaceuticals and the tests used for quality assurance, certifying that the product is fit for purpose. It is up to nuclear medicine centers deploy a program to raise the quality of exams and increase patient safety. (author)

  8. Poor Tc-99m dimercaptosuccinic acid uptake, re-evaluation with Tc-99m MAG3 scintigraphy in Lowe syndrome

    Science.gov (United States)

    Koca, Gokhan; Atilgan, Hasan Ikbal; Demirel, Koray; Diri, Akif; Korkmaz, Meliha

    2011-01-01

    Tc-99m dimercaptosuccinic acid (DMSA) is filtered through the glomeruli and reabsorbed by the proximal tubules as low molecular weight proteins. In Lowe syndrome this mechanism is impaired and so poor DMSA uptake is seen. Poor DMSA uptake was shown in very few studies, but none mentioned normal Tc-99m MAG3 uptake. In this case, the patient had poor DMSA uptake, normal MAG3 uptake and a neurogenic bladder in anterior to the left kidney that attenuates left kidney. PMID:23559713

  9. The radio-labeling of Ciprofloxacin with Technetium-99m

    International Nuclear Information System (INIS)

    Even with rapid technological development in the field of diagnostic imaging, the localization of infection continues to pose challenges in day-to-day routine clinical practice. Tc-99m Ciprofloxacin is a relatively new radiopharmaceutical, which has proven its utility in imaging infection. This paper presents a new method of labeling Ciprofloxacin with Tc-99m using SnCI2.2H20 as reducing agent. The procedure used 2 mg of Ciprofloxacin manufactured by Bayer, 400 μg of SnCI2.2H20 (Sigma Chemical Co.) and 185MBq of Technetium-99m in the form of pertechnetate (Tc-99mO4) in a volume of 300 μl. The labeling was carried out at 100 deg. C for 10 minutes and at ambient temperature for a similar period of time. The solution obtained was filtered using millipore filter of 0.22 μm size. The efficiency of the labeling, verified by ascending chromatography on Whatman No.1 paper was found to be 97.3 % (±1.6), while it was 96.8% (±2.3) using Whatman No.3 paper and 96.6% (± 2.1) using thin-layer chromatography. Chromatography by exclusion chromatography (Bio-Gel P 10) was used for confirmation of the above results. The labeled molecules were eluted first, followed by the molecules of Technetium-99m while the colloids remained attached to the column. The results of the present study are comparable with the results of previous studies reported in literature. (author)

  10. Production technologies for molybdenum-99 and technetium-99m

    International Nuclear Information System (INIS)

    Technetium-99m (6.02 h) is the most widely used radioisotope in nuclear medicine, accounting for more than 80% of all diagnostic nuclear medicine procedures. It is almost exclusively produced from the decay of its parent 99Mo. The present sources of 99Mo are research reactors by using the (n,γ) nuclear reaction with natural Mo (98Mo, ∼24%), resulting in inexpensive but low-specific activity 99Mo, or by neutron-induced fission of 235U, which results in expensive but high specific activity 99Mo. This publication covers several aspects related to the production of 99Mo and 99mTc. The contributed papers reflect the current status of the technology and discuss potential alternative methodologies for the production of 99Mo and 99mTc for medical use. The first four papers address the technologies using nuclear reactors, including the description of a new method using an aqueous homogenous reactor core for production of fission 99Mo and the latest development efforts to fabricate 235U low enriched targets (LEU, 235U). The next five papers discuss the potential of utilizing particle accelerators and assess the current status of the available nuclear data for the production of both, 99Mo and 99mTc with proton and deuteron beams. The last paper discusses a new technology based on gel system for the preparation of 99Mo/99mTc generators using low specific activity 99Mo produced in research reactors by the neutron activation of natural and inexpensive molybdenum oxide targets. Each individual paper was indexed and abstracted

  11. Differential Findings of Tc 99m Sestamibi Dual Phase Parathyroid Scintigraphy Between Benign and Malignant Parathyroid Lesions in Patients with Primary Hyperparathyroidism

    Energy Technology Data Exchange (ETDEWEB)

    Cheon, Miju; Choi, Joon Young; Chung, Jae Hoon; Lee, Ji Young; Cho, Sook Kyung; Yoo, Jang; Park, Soo Bin; Lee, Kyung Han; Kim, Byung Tae [Sungkyunkwan, Univ. School of Medicine, Seoul (Korea, Republic of)

    2011-12-15

    This study aimed to investigate the differential findings in clinical and biochemical features, and Tc 99m sestamibi (MIBI) dual phase parathyroid lesions in patients with primary hyperparathyroidism. Subjects were 102 parathyroid lesions from 91 patients with primary hyperparathyroidism. Scintigraphic findings included radioactivity grade, uptake pattern, uptake contour lesion size on early and delayed images, and degree of washout. Clinical and biochemical features were also evaluated. Histopathology confirmed the final diagnosis for all the patients. Final diagnoses were 94 benign parathyroid lesions and 8 parathyroid carcinomas. The patients with parathyroid carcinoma were significantly older (p=0.002) and had significantly higher serum parathyroid hormone concentrations than those with benign parathyroid lesions (p<0.001). All malignant parathyroid lesions showed intense radioactivity similar to or greater than the submandibular gland activity on delayed images (p=0.007), and little radioactivity difference between early and delayed images (p=0.012). The cancer incidence for parathyroid lesions with both intense radioactivity and no washout was 17.0% (8/47). When parathyroid lesions with all of the above mentioned findings were regarded as malignant, the cancer incidence significantly increased from 17.0% to 33.3% (8/24, p<0.001). For Tc 99m MIBI dual phase parathyroid scintigraphy, uptake grade on delayed images and washout were significantly useful diagnostic criteria for differentiating benign from malignant parathyroid lesions, along with age and parathyroid hormone serum concentration.

  12. [A new lymph node imaging agent--99mTc-polyphase liposome oleatis (99mTc-plo)].

    Science.gov (United States)

    Yu, B F

    1988-07-01

    A new lymph node radio-imaging agent, technetium-99m polyphase liposome oleatis (99mTc-plo), has recently been developed. Polyphase liposome oleatis was labelled with radionuclide by stannous chloride method. The labelled rate was 90% or more as technically identified by thin layer chromatography, external gamma-camera imaging and radioautography. In animal experiment, 0.2-0.3 ml (0.2 mci) of the 99mTc-plo was injected subcutaneously into the toes web of rats. After half an hour, the regional lymph nodes of popliteal fossa were visualized very clearly. The imaging figures may keep their distinct shadow up to 24 hours. The tested rabbits were sacrificed 10 hours after 99mTc-plo injection for detecting various kinds of tissue and organ with a scintillation counter. The regional lymph nodes revealed the highest uptake rate of the new agent, 12,116; 1,303; 1,615 times higher than that of the adjacent muscles, liver and spleen, respectively. In clinical experiment, 0.2-0.3 ml (0.5-0.8 mci) of the 99mTc-plo was injected subcutaneously into the toes web of patients. Half an hour later, the lymph nodes of inguinal, external iliac and common iliac regions appeared in sequence. If the new agent is injected perianally, the internal iliac lymph nodes will be seen. No side effect was observed in both types of experiment. This new agent has been tried in rats, rabbits and dogs with similar positive results. The new lymph node imaging technique is simple, safe, reliable and reproducible. This agent, being directed toward the lymph nodes and possessing affinity to cancer cells, is expected to be supplementary method to CT and B-ultrasonography for detecting lymphoid malignancy and lymph node metastasis. PMID:3248482

  13. 99m-technetium tetrofosmin and 99mTc-methylene diphosphonate in pre-surgical breast cancer

    International Nuclear Information System (INIS)

    Full text: The aim of this study was to assess the role of the Tc-99m Tetrofosmin (TF) in conjunction with Tc-99m methylenediphosphonate (MDP) in pre-surgical breast cancer (BC) staging. Thirty-six female patients, age range was 32-70 years (average 51.45 years), where the clinical examination, mammography (MG) and fine-needle aspiration cytology were inconclusive were subjected to Tc-99m TF Scintimammography (SMM). All the patients were examined in a specialized breast clinic by experienced surgeons and radiologists. Of the 36 patients, 24 had hyperdense breasts, 8 had undergone lumpectomy or mastectomy due to cancer in one of the breasts and were included in the present study because of suspicion of lesion in the contra lateral breast, 2 had palpable axillary lymph nodes (LN) but no palpable breast lump and 2 patients were of mastitis carcinomatosis. All biopsies were histopathologically verified 740 MBq of Tc-99m TF (Myoview-Amersham) was injected in the arm opposite to the side of the breast lesion. In patients with bilateral breast lesions (BL) radiopharmaceutical was injected in a pedal vein. Planar imaging in prone position was done 10-15 min later. Two lateral views of the left and right breasts including axilla were acquired followed by an anterior view in supine position with arm in an upright position so as to include both breasts and axillary region in the field of imaging view. Imaging was done using a large field of view single-head gamma camera (Diacam-Siemens) coupled with low-energy high-resolution collimator. In patients with locally advanced BC who were to receive pre-operative neo-adjuvant chemotherapy, cardiac GATED SPECT was also acquired using standard protocol. After few days, Tc-99m MDM scintimammography and whole-body bone scintigraphy was also performed. SMM, using the same acquisition protocol as with 99mTc-TF, was done 5-10 min after intravenous injection of 555-740 MBq 99mTc MDP. Standard WBBS was acquired two to three hours later. SMM

  14. Tc-99m ciprofioxacin imaging in acute cholecystitis

    Institute of Scientific and Technical Information of China (English)

    Yun-Mee Choe; Kee-Chun Hong; Seok-Hwan Shin; Kyung-Rae Kim; Ze-Hong Woo; Wonsick Choe; Keon-Young Lee; Seung-Ik Ahn; Kwang Kim; Young Up Cho; Sun Keun Choi; Yoon-Seok Hur; Sei-Joong Kim

    2007-01-01

    AIM: To evaluate the efficacy of a new nuclear imaging Infecton (Tc-99m ciprofioxacin) for the diagnosis of acute cholecystitis.METHODS: Sixteen patients thought to have acute cholecystitis were included in this study. The diagnosis of acute cholecystitis was made based on clinical symptoms and ultrasonographic and pathologic findings.RESULTS: The 16 patients were composed of 12 acute and 4 chronic cholecystitis patients. Twelve patients with acute cholecystitis were image-positive, including one false-positive. Four patients with chronic cholecystitis were image-negative, of whom three were true-negative.This nuclear imaging had a sensitivity of 91.7%, a specificity of 75%, a positive-predictive value of 91.7%,and a negative-predictive value of 75%.CONCLUSION: Tc-99m ciprofloxacin imaging is easy to perform and applicable for the diagnosis of acute cholecystitis.

  15. Tumor scintigraphy with sup(99m)Tc-tetracycline

    International Nuclear Information System (INIS)

    Of all radiopharmaceuticals, sup(99m)Tc-tetracycline has turned out to be the most efficient and to have a specific affinity for tumours. Due to its favourable physical properties, high doses can be given at relatively low radiation loads. Its pharmacological properties ensure that it is quickly eliminated from the rest of the body during enrichment in affinite tissue. Furthermore, it can be labelled at any time with an economical radionuclide. (GSE/AK)

  16. Tc99m-sestamibi dosimetry in myocardial perfusion imaging

    International Nuclear Information System (INIS)

    This paper addressed myocardial perfusion imaging providing a spatial dosimetric investigation of the 99mTc-radiopharmaceutical dose distribution at the myocardium. Radiological data manipulation was performed in order to create a computational voxel model of the heart. A set of images obtained by thoracic angiotomography and abdominal aorta was set up providing anatomic and functional information for heart modeling in SISCODES code. A homogeneous distribution of 99mTc was assumed into the cardiac muscle. Simulations of the transport of particles through the voxel and the interaction with the heart tissues were performed on the MCNP - Monte Carlo Code. The spatial dose distribution in the heart model is displayed as well as the dose versus volume histogram of the heart muscle. The present computational tools can generate spatial doses distribution in myocardial perfusion imaging. Specially, the dosimetry performed elucidates imparted dose distribution in the myocardial muscle per unit of injected 99m Tc activity, which can contribute to future deterministic effect investigations. (author)

  17. Technetium-99m in nuclear medicine and radiation protection experience

    Energy Technology Data Exchange (ETDEWEB)

    Ninkovic, M. M.; Raicevic, J. J.; Pavlovic, S.; Glisic, R. [Institute of Nuclear Sciences, Belgrade, Yugoslavia (Yugoslavia)

    2002-07-01

    The one of the major contributors to personnel exposure whithin nuclear medicine laboratories is primarily now ubiquitous {sup 99}Mo-{sup 99m}Tc generators. These generators, first available about more than forthy years ago, are now providing large quantities of {sup 99m} Tc radioactive source daily in the most laboratories, both for direct injection, as a per-technetate ion, and through the utilization of the kits for the preparation of dipherent radiopharmaceuticals. The safe and efficient utilization of technetium-99m in nuclear medicine, as other radionuclides, begin with the design, construction and operation of the 'Hot'Laboratory area, where radionuclide are stored and patient doses are prepared. Regardless of the specific physical configuration, suitable facilities need to be provided and good working practices established in order to get these principal objectives: (a) keep radiation exposure to personnel at ALARA levels; (b) prevent contamination of personnel; (c) prevent contamination of measuring instruments (including imaging devices such as gamma cameras and rectilinear scanners), and (d) prevent spread of contamination to places or persons outside the laboratory.

  18. Scintimammography with Technetium-99m-lipophilic cations in the diagnosis of breast cancer and lymph node metastasis

    International Nuclear Information System (INIS)

    The unfavorable outcome of breast cancer is related both to the frequency of the disease and to deficiencies in diagnosis, staging, prognostic characterization and therapeutic strategies. Mammography is the first diagnostic approach in the detection of breast masses, but it may yield false-negative and false-positive results, with a low accuracy in patients with dense breasts, implants, mastectomy or dysplasia. Ultrasonography mainly provides integrative information , but it does not significantly improve accuracy. Computed tomography is only rarely useful and magnetic resonance imaging is a promising method, but in a second-line role. Therefore, diagnostic invasive procedures such as fine needle aspiration and biopsy are still necessary, even if they can yield false negative or not adequate results due to sampling problems. Furthermore, none of these techniques is capable of reliable assessing axillary or internal lymph nodes involvement. Against this background, scintimammography may find a clinical role in a subset of patients. Promising results have been obtained with 99mTc-methylene diphosphonate and with tracer presenting cellular uptake such as 201Tl chloride, 99mTc-Sestamibi and 99mTc-Tetrofosmin. Sestamini (MIBI) and Tetrofosmin (TF) are lipophilic cations developed as myocardial perfusion agents. 99mTc-Mibi as well as 99mTc-TF have shown potential utility as a tumor imaging agent for parathyroid adenoma, lung cancer, thyroid cancer, soft tissue carcinoma and other tumors, Sensibility and specificity of scintimammography with 99mTc-Mibi are 85% and 89%, and with 99mTc-TF respectively 93% and 84%. We studies 46 patients with mammographic suspect of breast disease (age range 29-69 years, mean age 51). All patients underwent scintimammography 20 minutes after e.v. injection of 99mTc-TF 640-740 MBq in the arm contralateral to the affected breast planar scintigraphic images in supine anterior and prone lateral views are acquired. In order to minimize count

  19. Uptake of {sup 99m}Tc tetrofosmin in lymphoma cell lines: a comparative study with {sup 99m}Tc sestamibi

    Energy Technology Data Exchange (ETDEWEB)

    Ding, H.-J.; Shiau, Y.-C.; Tsai, S.-C.; Wang, J.-J.; Ho, S.-T.; Kao, Albert E-mail: albertkaotw@yahoo.com.tw

    2002-06-01

    Technetium-99m ({sup 99m}Tc) tetrofosmin has been used as a tumor-seeking agent. However, its role in detecting lymphomas has not been widely investigated. The aim of the present study was to determine the uptake and clearance characteristics of {sup 99m}Tc tetrofosmin in lymphoma cell lines. {sup 99m}Tc sestamibi was also evaluated for comparison. Three lymphoma cell lines (U-937: monocyte-like, histiocytic lymphoma, human; RAMOS: B-lymphoma cell line, American Burkitt lymphoma, lymphoblastoid, human; Hs445: Hodgkin's disease, lymphoid, human) were studied. After incubation of radiotracers {sup 99m}Tc tetrofosmin and {sup 99m}Tc sestamibi in medium for 0, 10, 20, 30, 60, 120 and 180 min, the uptake and clearance of each radiotracer were measured in the three lymphoma cell lines. The uptake of {sup 99m}Tc tetrofosmin was lower than that of {sup 99m}Tc sestamibi in these lymphoma cell lines. Among the three cell lines, Hs445 showed the greatest {sup 99m}Tc tetrofosmin uptake capacity. RAMOS and U-937 showed similar {sup 99m}Tc tetrofosmin uptake capacities. {sup 99m}Tc tetrofosmin accumulated in the three tested lymphoma cell lines, especially in the Hodgkin's disease cell line. However, in comparison with {sup 99m}Tc sestamibi, {sup 99m}Tc tetrofosmin may not be the best radiotracer for detection of lymphoma.

  20. Comparison of 99mTc-DTPA GFR and 99mTc-DTPA Renal Clearance%肾动态体外摄取法GFR与99mTc-DTPA肾清除率的对比研究

    Institute of Scientific and Technical Information of China (English)

    齐慧敏; 辛岗; 张春丽; 李驚子; 邓丽荣; 王海燕

    2002-01-01

    Objective To evaluate the two methods in GFR measurements. Methods By using Receiver Operating Characteristic (ROC) curve, likelihood ratio and regression analysis we compared 99mTc- DTPA GFR with clearance of 99mTc-DTPA (CL 99mTc). Results The 99mTc - DTPA GFR mean area under ROC curve was 0.79 mean sensitivity was 62%, mean specificity was 83.8%, r = 0.78, regression formula was Y = 8.5 + 0.76X. Conclusion The accuracy of 99mTc - DTPA was not so good.

  1. Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats

    International Nuclear Information System (INIS)

    This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1 ml of 99mTc-DMSA IV (0.48 MBq) was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g) determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (p<0.05) was done. There was a significant reduction in %ATI/g in kidney and blood (p<0.05) of splenectomized animals, a significant increase (p<0.05) of urea (88.8 ± 18.6 mg/dL) and creatinine (0.56 ± 0.08 mg/dL), compared to the controls (51.5±1.6, 0.37±0.02 mg/dL, respectively), as well as increase in platelets and leucocytes, and hematocrit reduction. The analysis of the results indicates that in rats, splenectomy seems to alter the renal function and the uptake of 99mTc-DMSA. (author)

  2. Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats

    Energy Technology Data Exchange (ETDEWEB)

    Acucena, Maria Kadja Meneses Torres; Pereira, Kercia Regina Santos Gomes; Villarim Neto, Arthur; Rego, Amalia Cinthia Meneses [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Centro de Ciencias da Saude; Bernardo-Filho, Mario [Universidade do Estado do Rio de Janeiro (UERJ), RJ (Brazil). Inst. de Biologia Roberto Alcantara Gomes. Dept. de Biofisica e Biometria; Azevedo, Italo Medeiros; Araujo Filho, Irami; Medeiros, Aldo Cunha [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Dept. de Cirurgia]. E-mail: aldo@ufrnet.br

    2008-12-15

    This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1 ml of 99mTc-DMSA IV (0.48 MBq) was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g) determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (p<0.05) was done. There was a significant reduction in %ATI/g in kidney and blood (p<0.05) of splenectomized animals, a significant increase (p<0.05) of urea (88.8 {+-} 18.6 mg/dL) and creatinine (0.56 {+-} 0.08 mg/dL), compared to the controls (51.5{+-}1.6, 0.37{+-}0.02 mg/dL, respectively), as well as increase in platelets and leucocytes, and hematocrit reduction. The analysis of the results indicates that in rats, splenectomy seems to alter the renal function and the uptake of 99mTc-DMSA. (author)

  3. Mandibular brown tumor revealing primary hyperparathyroidism. Contribution of the {sup 99}Tc-MIBI scintigraphy (report of case); Tumeur brune mandibulaire revelant une hyperparathyroidie primaire. Apport de la scintigraphie au {sup 99}Tc-MIBI: (a propos d'un cas)

    Energy Technology Data Exchange (ETDEWEB)

    Bahri, H.; Mhiri, A.; Zayed, S.; Letaief, B.; Slim, I.; Kraiem, T.; Ben Slimen, M.F. [Institut Salah Azaiez, Service de Medecine Nucleaire (Tunisia); Sellem, A.; Hammami, H. [Hopital Militaire Principal d' Instruction, Service de Medecine Nucleaire (Tunisia); Ladgham, A. [Institut Salah Azaiez, Service d' ORL (Tunisia)

    2006-06-15

    Thanks to the development of new diagnostic and therapeutic techniques, it has became rare to discover a primary hyperparathyroidism at the stage of renal and/or bony complications. The contribution of the {sup 99m}Tc-MIBI scintigraphy has been well described in the detection of the parathyroid adenoma but few publications showed its capacity to detect also brown tumors. We report a case of mandible brown tumor, revealing a primary hyperparathyroidism. {sup 99m}Tc-MDP scintigraphy, done in the setting of the bony lesion balance, showed the multifocal character of this tumor. {sup 99m}Tc-MIBI scintigraphy pointed out both parathyroid adenoma and brown tumor that fixed the radio tracer. (author)

  4. Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA in rats

    Directory of Open Access Journals (Sweden)

    Maria Kadja Meneses Torres Açucena

    2008-12-01

    Full Text Available This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6 and control (n = 6. After splenectomy (15 days, the administration of 0.1ml of 99mTc-DMSA IV (0.48 MBq was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (pEstudo com objetivo de avaliar se a esplenectomia altera a biodistribuição do 99mTc-DMSA e alguns parâmetros bioquímicos e hematológicos em ratos Wistar. Os animais forma divididos em 2 grupos: esplenectomizados (n=6 e controle(n=6. Após 15 dias, administração de 0,1 ml de 99mTc-DMSA via plexo orbital (0,48 MBq foi realizada. Rim, coração, pulmão, tireóide, estômago, bexiga e fêmur e amostras de sangue foram separadas. Após pesagem e contagem da radioatividade foi determinado o percentual de radioatividade/g (% ATI/g. Dosadas uréia e creatinina sérica, hematócrito, plaquetas e leucócitos. Estatística pelo teste t, significância 0,05 foi realizada. Foi observada redução significante no %ATI/g no rim e sangue (p<0,05 dos animais esplenectomizados, aumento significante (p<0.05 da uréia (88,8±18,6 mg/dL e creatinina (0,56±0,08, comparado aos controles (51,5±1,6; 0,37±0,02mg/dL, respectivamente assim como aumento de leucócitos e plaquetas e redução de hematócrito. Conclui-se que em ratos, a esplenectomia alterou a captação de 99mTc-DMSA pelo rim, e a função renal.

  5. Quantification of ocular inflammation with technetium-99m glucoheptonate

    International Nuclear Information System (INIS)

    Histological and morphometric evaluation of ocular inflammation is difficult, particularly when there is extensive ocular involvement with abscess formation and necrosis. A quantitative imaging procedure applicable to humans would be important clinically. To establish such a procedure, turpentine-induced ocular inflammation was obtained by subconjunctival injection in the right eye of 55 rabbits. The left eye was used as control and injected with a volume of saline equal to the volume of turpentine in the right eye. Volumes of turpentine or saline were 0.02, 0.04, 0.06, 0.2 and 0.6 ml, and the rabbits were divided into groups 1-5, according to these volumes. Imaging was performed 48 h after turpentine injection and 6 h after intravenous injection of 10 mCi of technetium 99m glucoheptonate (99mTc-GH). An inflammatory reaction index (IRI), defined as the ratio of counts of the right eye divided by counts of the left eye, was used. IRIs were proportional to the degree of inflammation and allowed the distinction of 3 subgroups: One represented by group 4, one by group 5 and one by groups 1, 2 and 3. This method of quantification of ocular inflammatory processes using 99mTc-GH is original, rapid, non-invasive, reproducible and safe, although unable to differentiate inflammatory processes caused by doses of turpentine which are very small and close to each other. It is conceivable that its application to humans will bring new insight into the ocular inflammatory process and response to therapy. (orig.)

  6. Sup(99m)Tc-DTPA radio aerosol ventilation study

    International Nuclear Information System (INIS)

    Papers on deposition pattern analyses of radioaerosol lung scans are few and not easy to interpret as there is a general lack of technical standardization and the number of patients studied is not large. Moreover these reports have been generally obtained with non-hydrosoluble radioaerosols. In the present study 43 patients underwent conventional pulmonary function testing and lung scanning using small droplet (equal or less than 2 microns) polydisperse sup(99m)Tc-DTPA radioaerosol produced with the ''Settling Bag System'' Medi 400 (Sorin). The scans were analysed by two methods: 1) a semiquantitative method proposed by Taplin (SQT); 2) an original simpler semiquantitative method (SQM). Correlations of SQT and SQM with FEV1 and MEF75 resulted highly significant (p<0,001). SQM proved to be superior so SQT as far as reproducibility is concerned. The following conclusions can be drawn: 1) small particle sup(99m)Tc-DTPA aerosol can be easily and cheaply produced by a commercial device; 2) such a radioaerosol is well suited for the evaluation of small and large airways patency, as reflected by MEF75 and FEV1 respectively, with a diagnostic yield comparable to non-hydrosoluble radioaerosols; 3) the original semiquantitative description of the deposition pattern proposed and validated in this study is quite easy to implement and it yields a high correlation with pulmonary function tests; moreover such an approach does not require digital data processing; 4) the sensitivity of sup(99m)Tc-DTPA for the diagnosis of bronchial obstruction is very high and superior to routine pulmonary function tests; in addition the ventilation scan allows the topographical localization of the obstruction themselves

  7. Sclerosing cholangitis: cholescintigraphy with Tc-99m-labeled DISIDA

    Energy Technology Data Exchange (ETDEWEB)

    Ament, A.E.; Bick, R.J.; Miraldi, F.D.; Haaga, J.R.; Wiedenmann, S.D.

    1984-04-01

    Four patients with primary sclerosing cholangitis (PCS) were examined with the hepatobiliary agent Tc-99m-labeled DISIDA (diisopropylphenylcarbamoyl iminodiacetic acid), and the results correlated with those of invasive cholangiography. Cholescintigraphy is superior to cholangiography in cases of suspected PSC where there is nonfilling of biliary radicals due to high-grade stenosis. The finding of delayed hepatic parenchymal clearance can allow estimation of the degree of obstruction of the various branches of the major bile ducts. Cholescintigraphy offers a noninvasive method of investigating patients with suspected sclerosing chloangitis, leading to earlier diagnosis. Confirmation with invasive cholangiographic procedures is recommended.

  8. Ventilation imaging with 99m technetium labeled aerosols

    International Nuclear Information System (INIS)

    The major clinical use of ventilation perfusion scintigraphy is for the diagnosis of pulmonary embolism (PE). Accurate diagnosis of PE is essential since effective treatment if available. The scintigraphic characteristic of PE are segmental perfusion defects in lung that is normally ventilated. The inherent shortcoming of perfusion scintigraphy is its lack of specificity. Combining a ventilation study with perfusion imaging improves the diagnostic specificity of lungs scintigraphy. This article describes clinical use of simple same day ventilation and perfusion imaging technique. The technique is based on a low dose ventilation procedure using 99m Tc labelled aerosol immediately followed by a standard dose perfusion procedure. (author)

  9. Technetium-99m-diethyl-IDA instant kit

    International Nuclear Information System (INIS)

    A formulation of stannous-diethyl-IDA freeze-dried kit, containing 50 mg diethyl-IDA and 0.4 mg hydrated stannous chloride, to be labelled with technetium was developed for hepatobiliary scintigraphy. The organ distribution data of 99mTc-diethyl-IDA in mice for 60 min post injection were satisfactory. The radiopharmaceutical exhibits rapid blood clearance, great hepatic clearance and very short hepatocyte transit time. Uptake of the radiopharmaceutical was highest in mouse liver and intestine. The renal uptake of the HB agent in mice is relatively low. Blood clearance data showed that the HB agent is rapidly cleared. (author) 20 refs.; 4 tabs

  10. Technetium 99m pertechnetate thyroid scintigraphy: Congenital hypothyroid screening

    International Nuclear Information System (INIS)

    Technetium 99m pertechnetate thyroid scans were performed on 57 infants referred for evaluation of suspected congenital hypothyroidism. Thyroid anatomy may be characterized by four general types, based on the scintigraphic findings: (1) normal size and location. (2) ectopic location. (3) no detectable thyroid activity. (4) normal location with increased size or uptake. There are diverse etiologies of congential hypothyroidism. Correlation of thyroid scintigraphy with blood T4 and TSH levels allows specific etiological diagnosis in the majority of cases of congential hypothyroidism. (orig.)

  11. Technetium 99m pertechnetate scans in congenital hypothyroidism

    International Nuclear Information System (INIS)

    Goiters are rarely palpable in infants with congenital hypothyroidism except in the case of maternal ingestion of iodide. The presence or absence of glandular tissue is, however, important for genetic and prognostic counseling and for acceleration of diagnosis in other affected siblings. The detection of thyroid tissue by /sup 99m/Tc pertechnetate scans in a significant number of our patients heretofore considered athyreotic establishes that physical findings and traditional laboratory data are not adequate to determine whether or not thyroid tissue is present

  12. 99Tcm-MIBI evaluation of myeloma

    International Nuclear Information System (INIS)

    Full text: Preliminary reports of increased bone marrow uptake of 99Tcm-MIBI in myeloma have demonstrated the potential for assessment of activity of the disease. A series of 27 patients with myeloma in various stages of activity ranging from monoclonal gammopathies of uncertain significance (MGUS) and myeloma in remission or plateau phase to active or relapsed disease were compared with 30 control patients with normal bone marrow. Whole-body images were acquired 10 min following intravenous administration of 900 MBq 99Tcm-MIBI. The whole-body distribution of activity in 30 patients being routinely followed up for thyroid carcinoma, in whom there was no evidence of disease, were used as normal controls against which the myeloma patient scans were compared. Diffuse homogeneous uptake of 99Tcm-MIBI was evident throughout the bone marrow of spine, pelvis or sternum in two-thirds of controls. The 99Tcm-MIBI study was deemed positive in the myeloma patients only if diffuse bone marrow uptake was more intense or extensive than in controls, or if focal abnormalities were observed. Of 20 patients with clinical evidence of active myeloma, the99Tcm-MIBI study was positive in 18, while the 99Tcm-MIBI study was negative in 6 of the 7 patients with MGUS or myeloma in remission or plateau phase. These results support the use of 99Tcm-MIBI as an accurate means of assessment of myeloma disease activity

  13. Evaluation of adenosine preconditioning with {sup 99m}Tc-His{sub 10}-annexin V in a porcine model of myocardium ischemia and reperfusion injury: preliminary study

    Energy Technology Data Exchange (ETDEWEB)

    Ye Fei [Department of Cardiology, Nanjing First Hospital Affiliated to Nanjing Medical University, 68 Changle Road, Nanjing 210006 (China); Fang Wei [Cardiovascular Institute and Fuwai Hospital, No. 167 Bei-Li-Shi-Lu, Beijing 100037 (China); Wang Feng, E-mail: fengwang1972cn@gmail.co [Department of Nuclear Medicine, Nanjing First Hospital Affiliated to Nanjing Medical University, 68 Changle Road, Nanjing 210006 (China); State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, College of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093 (China); Hua Zichun [State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, College of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093 (China); Wang Zizheng [Department of Nuclear Medicine, Nanjing First Hospital Affiliated to Nanjing Medical University, 68 Changle Road, Nanjing 210006 (China); Yang Xiang [State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, College of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093 (China)

    2011-05-15

    Purpose: The goal of this study was to evaluate the feasibility of {sup 99m}Tc-His{sub 10}-annexin V for the detection of acute myocardial cell death and to assess the effect of adenosine preconditioning in a porcine model of myocardium ischemia and reperfusion injury (RI). Materials and Methods: {sup 99m}Tc-His{sub 10}-annexin V was prepared by one-step direct labeling, and RCP and radiostability were tested. The binding of {sup 99m}Tc-His{sub 10}-annexin V to apoptosis was validated in vitro using camptothecin-induced Jurkat cells. In vivo biodistribution was determined in mice by the dissection method. Ischemia of 20-30 min was induced by balloon occlusion of the epicardial coronary artery of the porcine model (n=14). Adenosine was infused intravenously in six pigs before coronary occlusion. {sup 99m}Tc-His{sub 10}-annexin V (n=12) was injected intravenously at 1 h after reperfusion. SPECT/CT was acquired at 3 h postinjection. Myocardial perfusion imaging (MPI) with {sup 99m}Tc-MIBI was also performed 1 day after His{sub 10}-annexin V imaging. Cardiac tissues were analyzed postmortem using hematoxylin-and-eosin and TUNEL staining. Caspase-3 activity was measured to confirm the presence of apoptosis. Results: {sup 99m}Tc-His{sub 10}-annexin V had a RCP >98% and high stability 2 h after radiolabeling; it could bind to apoptotic cells with high affinity. Biodistribution of {sup 99m}Tc-His{sub 10}-annexin V showed a predominant uptake in the kidney and relatively low uptake in the myocardium, liver and gastrointestinal tract; rapid clearance from blood and kidney was observed. In the untreated group, intense uptake of His{sub 10}-annexin V was visualized in the defect which was shown in MPI, whereas in the adenosine group a mild uptake of {sup 99m}Tc-His{sub 10}-annexin was found in the risk area which showed no defects in the {sup 99m}Tc-MIBI image. TUNEL staining and activated caspase-3 confirmed the ongoing apoptosis in RI. Adenosine preconditioning significantly

  14. Technetium-99m NGA functional hepatic imaging: preliminary clinical experience

    International Nuclear Information System (INIS)

    Technetium-99m galactosyl-neoglycoalbumin ( [Tc]NGA) is a radiolabeled ligand to hepatic binding protein, a receptor which resides at the plasma membrane of hepatocytes. This receptor-binding radiopharmaceutical and its kinetic model provide a noninvasive method for the assessment of liver function. Eighteen patients were studied: seven with hepatoma, eight with liver metastases, four with cirrhosis, and one patient with acute fulminant non-A, non-B hepatitis. Technetium-99m NGA liver imaging provided anatomic information of diagnostic quality comparable to that obtained with other routine imaging modalities, including computed tomography, angiography, ultrasound, and [Tc]sulfur colloid scintigraphy. Kinetic modeling of dynamic [Tc]NGA data produced estimates of standardized hepatic blood flow, Q (hepatic blood flow divided by total blood volume), and hepatic binding protein concentration, [HBP]. Significant rank correlation was obtained between [HBP] estimates and CTC scores. This correlation supports the hypothesis that [HBP] is a measure of functional hepatocyte mass. The combination of decreased Q and markedly reduced [HBP] may have prognostic significance; all three patients with this combination died of hepatic failure within 6 wk of imaging

  15. Biodistribution of the radiopharmaceutical technetium-99m-sodium phytate in rats after splenectomy

    Directory of Open Access Journals (Sweden)

    Kércia Regina Santos Gomes Pereira

    2008-12-01

    Full Text Available Drugs and surgery can interfere with the biodistribution of radiopharmaceuticals and data about the effect of splenectomy on the metabolism of phytate-Tc-99m are scarce. This study aimed at evaluating the interference of splenectomy on phytate-Tc-99m biodistribution and liver function in rats. The SP group rats (n=6 underwent splenectomy. In group C (control the animals were not operated on. After 15 days, all rats were injected with 0.1mL of Tc-99m-phytate via orbital plexus (0.66MBq. After 30 minutes, liver samples were harvested, weighed and the percentage of radioactivity per gram (%ATI/g was determined by a Wizard Perkin-Elme gama counter. The ATI%/g in splenectomized rats (0.99±0.02 was significantly higher than in controls (0.4±0.02, (p=0.034. ALT, AST and HDL were significantly lower in SP rats (p= 0.001 and leukocytosis was observed in SP rats. In conclusion, splenectomy in rats changed the hepatic biodistribution of Tc-99m-phytate and liver enzimatic activity.O radiofármaco fitato-Tc-99m é usado no diagnóstico através de exames de imagem, na dependência de sua biodistribuição. O objetivo do trabalho foi avaliar efeito da esplenectomia na biodistribuição do fitato-Tc-99m e função hepática em ratos Wistar. Sob anestesia e técnica asséptica, os animais do grupo SP (n=6 foram esplenectomizados. Grupo C(controle; n=6 não operado. Após 15 dias, injeção de 0,1ml de fitato-Tc-99m via plexo orbital (0,66MBq. Após 30 minutos, retiradas biópsias hepáticas para determinação do percentual de radioatividade/grama (% ATI/g, usando-se contador gama WizardPerkin-Elmer®. Realizada dosagem de ALT, AST e HDL, e leucometria. Estatística pelo teste t, significância 0,05. O %ATI/g nos ratos esplenectomizados foi 0,99 ± 0,2 e nos controles 0,40 ± 0,2 (p=0,034. ALT, AST e HDL tiveram dosagens significativamente menores nos esplenectomizados (p=0,01, com leucocitose, comparando com controles. Em conclusão, em ratos a esplenectomia

  16. Using radioisotopes compounds (99mTc-infecton and 99mTcHIG) in evaluating and examination of pulmonary tuberculosis patients

    International Nuclear Information System (INIS)

    This study examined 40 patients: 34 have pulmonary tuberculosis, 3 have pneumonitis, 2 have lung cancer, and one has bilharziasis. All patients have been imaged using radioisotopes compounds (99mTc-infecton and 99mTcHIG) and after 1 hour and 4 hours of patients injection.Using 99mTc-infecton gave positive results in 30 pulmonary tuberculosis patients out of 34, the 3 pneumonitis patients, while using 99mTcHIG gave positive results in 27 pulmonary tuberculosis patients including the 3 pneumonitis patients. Both 99mTc-infecton and 99mTcHIG gave negative results with the lung cancer patients and unreal positive results with the bilharziasis patient. It has been found that the sensitivity and accuracy in the examinations using 99mTc-infecton were 88%, 93%, and 85.7% respectively in comparison with using 99mTcHIG where the values were 70.6%, 91%, and 68.5% respectively. Continuing examination and monitoring of 18 tuberculosis patients for 2-18 months with anti-pulmonary tuberculosis treatment, showed complete response of 12 patients using 99mTc-infecton, and 8 by using 99mTcHIG. As a result, 99mTc-infecton can be used for examining pulmonary tuberculosis patients

  17. Study on 99mTc-MAG3 and 99mTc-DMSA renal accumulation using in vitro cellular model.

    Science.gov (United States)

    Nový, Zbynĕk; Mandíková, Jana; Trejtnar, Frantisek

    2011-02-01

    Mercaptoacetyltriglycine (MAG3) and dimercaptosuccinic acid (DMSA) labelled with technetium-99m belongs to standard renal radiodiagnostics. However, the renal transport mechanisms responsible for their high renal uptake have not been fully explained. In addition, no in vitro experimental study comparing the renal uptake of these radiopharmaceuticals at the cellular level has not been performed. The investigation compared the 99mTc-MAG3 and 99mTc-DMSA renal uptake using primary rat renal cells and evaluated contribution of active and passive transport processes to the renal accumulation. The renal cells were isolated from the rat kidneys by means of the two-phase collagenase perfusion method. The used experimental model showed to be useful tool for such type of investigation. The results documented significant quantitative and qualitative differences in the accumulation of 99mTc-DMSA and 99mTc-MAG3 in the rat isolated cells. The found experimental data indicated several times higher uptake of 99mTc-MAG3 than that found in 99mTc-DMSA. 99mTc-MAG3 cellular uptake was substantially decreased when active, energy-dependent processes were inhibited. However, 99mTc-DMSA accumulation in the renal cells demonstrated only a minor dependency on energy. These findings demonstrate a very different character of the membrane transport determining 99mTc-DMSA and 99mTc-MAG3 renal accumulation.

  18. Radiopharmacokinetic data for 99mTc-ABP - A new radiopharmaceutical for bone scanning: Comparison with 99mTc-MDP

    International Nuclear Information System (INIS)

    Technetium-99m-labeled alendronate is a new radiopharmaceutical for bone scanning developed under strict quality control at the INNSZ. The purpose of this work was to compare the radiopharmacokinetic data and the dosimetry of 99mTc-ABP and 99mTc-MDP in 10 volunteers, after it was tested in laboratory animals. 99mTc-ABP has shorter mean residence time (MRT) and t(1(2)) β; is less protein bound; has a higher renal clearance; smaller Vdss, and similar bone uptake at 1 and 2 h. 99mTc-ABP gives less radiation exposure to the patient with a 740 MBq dose, and the quality of the bone scan is excellent. 99mTc-ABP is a better radiopharmaceutical than 99mTc-MDP for bone scanning

  19. Estudo experimental de linfonodo sentinela na mama da cadela com azul patente e Tecnécio Tc99m Experimental study of the sentinel lymph node in the dog breast using blue dye and technetium Tc99m

    Directory of Open Access Journals (Sweden)

    Luiz Gonzaga Porto Pinheiro

    2003-12-01

    Full Text Available Pesquisa do Linfonodo Sentinela (LS tem se mostrado efetiva na avaliação axilar nas portadoras de neoplasia mamária. O LS negativo torna desnecessário o esvaziamento axilar. OBJETIVO: Identificar o LS da região subareolar da mama em cadelas utilizando corante azul patente (AP, Tecnécio Tc99m ou a associação de ambas as técnicas e compará-las quanto à sensibilidade na detecção do linfonodo sentinela. MÉTODOS: Foram estudadas dezessete cães fêmeas. Um total de 55 mamas foram analisadas. Utilizou-se AP (2,5%/0,5 ml e/ou Tc99m (1,0 mC/0,8ml para identificação do LS. A aplicação do Tc99m era realizada duas horas antes da realização do experimento. O AP era injetado na região subareolar da mama. Localizado o LS, realizava-se sua exérese. RESULTADOS: Dos 44 linfonodos em que se utilizou AP, pôde-se verificar que 40 estavam corados (90,90%. Dos 48 linfonodos em que se utilizou Tc99m, 47 linfonodos estavam radioativos (97,91% (p=0.18; k= - 0.067. Nas 37 mamas em que se associou AP ao Tc99m, apesar de 02 linfonodos não estarem corados, todos estavam radioativos (AP vs AP+Tc: p=0.12; k=0.083; Tc vs AP+Tc: p=1.0; k=0.018. CONCLUSÃO: O Tc99m e o corante azul patente, isolados ou associados, prestam-se à identificação do LS da mama do animal.The sentinel lymph node research (SLN has been effective in the evaluation of nodal status in patients with breast cancer. A negative SLN makes an axillary lymphadenectomy unnecessary. PURPOSE: To identify the SLN of the subareolar region in female dog breasts using blue dye (BD, Technetium (Tc99m or the association of both techniques and to compare their sensibility in the detection of the SLN. METHODS: Seventeen female dogs were studied. 55 breasts were analyzed. DB and/or Tc99m were used for the identification of SLN. Tc99m was introduced two hours before the experiment. BD was introduced some minutes before the procedure. Once the SLN was localized its dissection was performed. RESULTS

  20. 99mTc-MDP全身骨扫描在肾性骨病中的显像特点探讨

    Institute of Scientific and Technical Information of China (English)

    程旭; 李永军; 徐兆强; 袁孝军; 许昕丹; 包丽华

    2014-01-01

    目的:探讨99mTc标记的亚甲基二磷酸盐(99mTc-methylene diphosphonate,99mTc-MDP)全身骨扫描在肾性骨病患者中的显像特点.方法:65例临床证实慢性肾功能衰竭的患者均接受99mTc-MDP全身骨扫描、血尿素氮、血肌酐和甲状旁腺激素测定.对骨扫描图像进行定性分析和半定量评分,评价代谢性骨病的特征.部分患者(33例)同时接受99mTc标记的甲氧基异丁基异腈(99mqc-methoxyisobutylisonitrile,99mTc-MIBI)双时相甲状旁腺显像,对所得图像进行分析.结果:65例中,分别有28、27、9和1例具备5、4、3和2个代谢性骨病征象.具有双肾显影异常特征的患者数最多(61例),具备领带样胸骨特征的患者数最少(13例).半定量评分总分为4、5、6、7、8、9和10分的患者数分别为6、13、8、5、14、11和8例.33例同时接受99mTc-MIBI双时相甲状旁腺显像患者图像上均可见甲状旁腺机能亢进组织.结论:99mTc-MDP全身骨扫描在肾性骨病中具有其显像特点,结合半定量评分等有助于为肾性骨病患者的诊断、疗效和预后评价提供有价值的信息.

  1. Synthesis and characterisation of technetium-99m labelled ciprofloxacin (Infecton)

    International Nuclear Information System (INIS)

    Full text: Infecton is Tc-99m labelled Ciprofloxacin, which is a synthetic carboxyquinolone derivative with broad-spectrum antimicrobial activity. It is a new class of radiopharmaceutical designed for imaging live bacterial infection. Conventional imaging agents such as Tc-99m or In-111 labelled leucocytes are either time-consuming or hazardous due to blood handling. These can be obviated by the use of Infecton which we synthesised by modifying the procedure described by Britton and co-workers (Lancet 1996;..347: 233-235). Bioassay and animal studies have been performed with a view to its use in infection imaging in patients. The first task was to prepare pure ciprofloxacin from its commercially available lactate salt. This was achieved by isoelectric precipitation at pH 8.6 by adding sodium hydroxide to the formulation. The resultant precipitate was washed with 200 mL of water for injection and filtered through a cintered-glass filtering unit. The precipitate was free of lactate and sodium as analysed by Biochem analyser. The UV spectrophotometric analysis showed an absorption peak at 276.3 nm which is close to the theoretical value of 277 nm, thus confirming the purity of the compound. Infecton was synthesised by adding 2 mg of pure ciprofloxacin, 0.4 mg of formimidine sulfinic acid ( non-stannous reducing agent) and 1.0 GBq Tc-99m pertechnetate in a final volume of 1.0 mL saline into a sterile N2-filled Amersham vial and boiling the mixture at 100 deg C for 10 min. The purity of the product was 40-45%. It was passed through a DEAE Sephadex A-25 column and eluted with phosphate buffered saline (0.01M, pH 6.9) with a purity of >96%. The preparation was characterised by bioassay (n=3) by adding Infecton to four different broths of bacterial strains viz Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and MRSA with resultant binding of 50.4, 45.6, 48.5 and 50.3% respectively. The binding was less than 1.0% when either ciprofloxacin or

  2. 99mTc: Labeling Chemistry and Labeled Compounds

    Science.gov (United States)

    Alberto, R.; Abram, U.

    This chapter reviews the radiopharmaceutical chemistry of technetium related to the synthesis of perfusion agents and to the labeling of receptor-binding biomolecules. To understand the limitations of technetium chemistry imposed by future application of the complexes in nuclear medicine, an introductory section analyzes the compulsory requirements to be considered when facing the incentive of introducing a novel radiopharmaceutical into the market. Requirements from chemistry, routine application, and market are discussed. In a subsequent section, commercially available 99mTc-based radiopharmaceuticals are treated. It covers the complexes in use for imaging the most important target organs such as heart, brain, or kidney. The commercially available radiopharmaceuticals fulfill the requirements outlined earlier and are discussed with this background. In a following section, the properties and perspectives of the different generations of radiopharmaceuticals are described in a general way, covering characteristics for perfusion agents and for receptor-specific molecules. Technetium chemistry for the synthesis of perfusion agents and the different labeling approaches for target-specific biomolecules are summarized. The review comprises a general introduction to the common approaches currently in use, employing the N x S4-x , [3+1] and 2-hydrazino-nicotinicacid (HYNIC) method as well as more recent strategies such as the carbonyl and the TcN approach. Direct labeling without the need of a bifunctional chelator is briefly reviewed as well. More particularly, recent developments in the labeling of concrete targeting molecules, the second generation of radiopharmaceuticals, is then discussed and prominent examples with antibodies/peptides, neuroreceptor targeting small molecules, myocardial imaging agents, vitamins, thymidine, and complexes relevant to multidrug resistance are given. In addition, a new approach toward peptide drug development is described. The section

  3. Cardiac amyloidosis detection with pyrophosphate-99mTc scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Souza, D.S.F.; Ichiki, W.A.; Coura Filho, G.B.; Izaki, M.; Giorgi, M.C.P.; Soares Junior, J; Meneghetti, J.C. [Universidade de Sao Paulo (FM/USP), SP (Brazil). Fac. de Medicina. Instituto do Coracao. Servico de Medicina Nuclear e Imagem Molecular

    2008-07-01

    Full text: Introduction: Amyloidosis is a rare disease, characterized by extracellular deposition of insoluble amyloid fibrils in organs and tissues. It may affect virtually any system, preferably heart, kidneys and liver. The cardiac involvement produces a spectrum of clinical features, usually with progressive dysfunction. Early diagnosis is important for institution of appropriate therapy. Case report: Male patient, 75 years old, with diagnosed congestive heart failure functional class III and Mobitz II second-degree atrial-ventricular block, was hospitalized for implantation of definitive cardiac pacemaker. Patient mentioned history of worsening effort dyspnoea over a one-month period, progressing to minimum effort, orthopnea, paroxysmal nocturnal dyspnoea and paroxysms of dry cough, and swelling of lower limbs. Echocardiography showed diffuse hypertrophy of left ventricle (LV), with systolic dysfunction due to diffuse hypokinesia and hyperrefringent aspect in the septum. It was questioned a cardiac infiltrating process. Cardiac amyloidosis was considered as a diagnostic hypothesis. The patient underwent a pyrophosphate-{sup 99m}Tc scintigraphy, which showed abnormal tracer uptake in the heart projection, with diffuse pattern on the left ventricle walls, compatible with the clinical suspicion cardiac amyloidosis, which was later confirmed by endomyocardial biopsy. Discussion: In this case report, the patient had clinical and other auxiliary examinations, such as electrocardiography and Doppler echocardiography, compatible with cardiac amyloidosis, which led to implementation with pyrophosphate-{sup 99m}Tc scintigraphy and later endomyocardial biopsy. Cardiac amyloidosis occurs in about half the cases of primary amyloidosis (AL) and is rare in secondary amyloidosis (AA). Its clinical presentation is polymorphic and it can be classified into four distinctive types: restrictive cardiomyopathy, systolic dysfunction, postural hypotension and conduction disorders

  4. Synthesis and characterisation of technetium-99m labelled ciprofloxacin (Infecton)

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, V.; Choong, K.K.L.; Olma, T.R.; Mieczkowski, S. [Westmead and the New Childrens Hospital, Westmead, NSW (Australia). Department of Nuclear Medicineand Ultrasound and Centre for infectious Diseases and Microbiology laboratory Services

    1998-06-01

    Full text: Infecton is Tc-99m labelled Ciprofloxacin, which is a synthetic carboxyquinolone derivative with broad-spectrum antimicrobial activity. It is a new class of radiopharmaceutical designed for imaging live bacterial infection. Conventional imaging agents such as Tc-99m or In-111 labelled leucocytes are either time-consuming or hazardous due to blood handling. These can be obviated by the use of Infecton which we synthesised by modifying the procedure described by Britton and co-workers (Lancet 1996;..347: 233-235). Bioassay and animal studies have been performed with a view to its use in infection imaging in patients. The first task was to prepare pure ciprofloxacin from its commercially available lactate salt. This was achieved by isoelectric precipitation at pH 8.6 by adding sodium hydroxide to the formulation. The resultant precipitate was washed with 200 mL of water for injection and filtered through a cintered-glass filtering unit. The precipitate was free of lactate and sodium as analysed by Biochem analyser. The UV spectrophotometric analysis showed an absorption peak at 276.3 nm which is close to the theoretical value of 277 nm, thus confirming the purity of the compound. Infecton was synthesised by adding 2 mg of pure ciprofloxacin, 0.4 mg of formimidine sulfinic acid ( non-stannous reducing agent) and 1.0 GBq Tc-99m pertechnetate in a final volume of 1.0 mL saline into a sterile N{sub 2}-filled Amersham vial and boiling the mixture at 100 deg C for 10 min. The purity of the product was 40-45%. It was passed through a DEAE Sephadex A-25 column and eluted with phosphate buffered saline (0.01M, pH 6.9) with a purity of >96%. The preparation was characterised by bioassay (n=3) by adding Infecton to four different broths of bacterial strains viz Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and MRSA with resultant binding of 50.4, 45.6, 48.5 and 50.3% respectively. The binding was less than 1.0% when either ciprofloxacin or

  5. Different uptake of {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO in the same brains: analysis by statistical parametric mapping

    Energy Technology Data Exchange (ETDEWEB)

    Hyun, I.Y. [Dept. of Nuclear Medicine, Inha University College of Medicine, Incheon (Korea); Lee, J.S.; Lee, D.S. [Dept. of Nuclear Medicine, Seoul National University College of Medicine, Seoul (Korea); Rha, J.H.; Lee, I.K.; Ha, C.K. [Dept. of Neurology, Inha University College of Medicine, Incheon (Korea)

    2001-02-01

    The purpose of this study was to investigate the differences between technetium-99m ethyl cysteinate dimer ({sup 99m}Tc-ECD) and technetium-99m hexamethylpropylene amine oxime ({sup 99m}Tc-HMPAO) uptake in the same brains by means of statistical parametric mapping (SPM) analysis. We examined 20 patients (9 male, 11 female, mean age 62{+-}12 years) using {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO single-photon emission tomography (SPET) and magnetic resonance imaging (MRI) of the brain less than 7 days after onset of stroke. MRI showed no cortical infarctions. Infarctions in the pons (6 patients) and medulla (1), ischaemic periventricular white matter lesions (13) and lacunar infarction (7) were found on MRI. Split-dose and sequential SPET techniques were used for {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO brain SPET, without repositioning of the patient. All of the SPET images were spatially transformed to standard space, smoothed and globally normalized. The differences between the {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO SPET images were statistically analysed using statistical parametric mapping (SPM) 96 software. The difference between two groups was considered significant at a threshold of uncorrected P values less than 0.01. Visual analysis showed no hypoperfused areas on either {sup 99m}Tc-ECD or {sup 99m}Tc-HMPAO SPET images. SPM analysis revealed significantly different uptake of {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO in the same brains. On the {sup 99m}Tc-ECD SPET images, relatively higher uptake was observed in the frontal, parietal and occipital lobes, in the left superior temporal lobe and in the superior region of the cerebellum. On the {sup 99m}Tc-HMPAO SPET images, relatively higher uptake was observed in the medial temporal lobes, thalami, periventricular white matter and brain stem. These differences in uptake of the two tracers in the same brains on SPM analysis suggest that interpretation of cerebral perfusion is possible using SPET with {sup 99m}Tc-ECD and

  6. Different uptake of 99mTc-ECD and 99mTc-HMPAO in the same brains: analysis by statistical parametric mapping

    International Nuclear Information System (INIS)

    The purpose of this study was to investigate the differences between technetium-99m ethyl cysteinate dimer (99mTc-ECD) and technetium-99m hexamethylpropylene amine oxime (99mTc-HMPAO) uptake in the same brains by means of statistical parametric mapping (SPM) analysis. We examined 20 patients (9 male, 11 female, mean age 62±12 years) using 99mTc-ECD and 99mTc-HMPAO single-photon emission tomography (SPET) and magnetic resonance imaging (MRI) of the brain less than 7 days after onset of stroke. MRI showed no cortical infarctions. Infarctions in the pons (6 patients) and medulla (1), ischaemic periventricular white matter lesions (13) and lacunar infarction (7) were found on MRI. Split-dose and sequential SPET techniques were used for 99mTc-ECD and 99mTc-HMPAO brain SPET, without repositioning of the patient. All of the SPET images were spatially transformed to standard space, smoothed and globally normalized. The differences between the 99mTc-ECD and 99mTc-HMPAO SPET images were statistically analysed using statistical parametric mapping (SPM) 96 software. The difference between two groups was considered significant at a threshold of uncorrected P values less than 0.01. Visual analysis showed no hypoperfused areas on either 99mTc-ECD or 99mTc-HMPAO SPET images. SPM analysis revealed significantly different uptake of 99mTc-ECD and 99mTc-HMPAO in the same brains. On the 99mTc-ECD SPET images, relatively higher uptake was observed in the frontal, parietal and occipital lobes, in the left superior temporal lobe and in the superior region of the cerebellum. On the 99mTc-HMPAO SPET images, relatively higher uptake was observed in the medial temporal lobes, thalami, periventricular white matter and brain stem. These differences in uptake of the two tracers in the same brains on SPM analysis suggest that interpretation of cerebral perfusion is possible using SPET with 99mTc-ECD and 99mTc-HMPAO. (orig.)

  7. Scintimammography with Tc-99m sestamibi in breast cancer

    International Nuclear Information System (INIS)

    Full text: It is already known that breast cancer is the most common malignancy in women. Physical breast examination and mammography are currently the most recognized screening tools for detection of breast cancer. However, breast mammography has some limitations, especially in women with dense breast or in patients who have had surgery, radiotherapy or chemotherapy. The aim of this study was to investigate the role of Tc-99m Sestamibi scintimammography in the diagnosis of breast cancer. Forty-two women (median age= 46 years, range 21-78 years) were investigated. All had a thorough clinical examination, ultrasonography, mammography, determination of the level of tumor markers - CA15.3 and CEA, fine needle aspiration cytology (FNA) and scintimammography before surgery. The final diagnosis was determined by histopathological examination. For scintimammography, a dose of 740 MBq of Tc-99m sestamibi was injected into a foot vein followed by 10ml saline flush. Imaging was performed 10 minutes later in prone position; lateral views of the breasts and axilla were taken (10 minutes per view, matrix size 128x128, high resolution collimator). Anterior views of the breast and axillary areas were also acquired with patients in supine position. Delayed scintigraphy in prone position (lateral view) was done 60 minutes after injection. Focal accumulation of sestamibi in tumor on early and delayed scintigraphy was considered positive. The results of scintimammography were compared with histopathology. The histopathological results revealed malignancy in 33 (78.6%) and benign tumors in 9 (21.4%) cases. Scintimammography showed true positive findings in 31 (73.8%) patients having breast cancer. It was false negative in 2 cases (1 of invasive ductal carcinoma and 1 of invasive lobular carcinoma). All 5 cases with nonpalpable invasive ductal carcinoma and two cases with ductal carcinoma in-situ were positive on scintimammography. True negative findings with no focally increased

  8. Somatostatin analogues labelled with 99mTc

    International Nuclear Information System (INIS)

    Biological and radiochemical studies have been carried out on two labelled somatostatin analogues, the peptide RC-150 and the Tyr3-Octreotide. Both analogues have been labelled with 99mTc using the direct and the indirect method and MAG-3 and HYNIC as chelating agents. By the direct method RC-150 was labelled using sodium ascorbate and dithionite as reducing agents. The radiochemical purity was 70%. By the indirect method, in the case of RC-160 with MAG-3 a radiochemical purity higher than 70% was attained while a purity of 100% was reached in the case of Tyr3-Octreotide with HYNIC. The biological distribution of HYNIC-Tyr3-Octreotide has been studied in rats. (author)

  9. Study of technetium behaviour in radiopharmaceuticals. Characterization of sup(99m)Tc-pyrophosphate, sup(99m)Tc-dimercaptosuccinate, sup(99m)Tc-diethylenetriaminepentaacetate complexes and sup(99m)Tc-colloidal rhenium sulphide

    International Nuclear Information System (INIS)

    The chemistry of technetium in extremely dilute solution was approached through the study of three complexing agents and a colloid. By the application of high-performance chromatographic techniques to the analysis of (Tc-pyro), (Tc-DTPA), (Tc-DMSA) complexes it was possible to isolate one or more chelates from a single complexing agent. Addition of pertechnetates to a solution of sodium pyrophosphates and stannous chloride at neutral pH leads to the formation of two complexes, both highly osteotropic. By the use of sup(117m)Sn it was shown that tin employed as reducing agent enters into the composition of one of the two complexes, either of which may be obtained preferentially by varying the (Sn)/(pyro) ratio. With technetium at acid pH (2.5) DMSA gives one or more chelates according to the concentration of the reagents present. DTPA with technetium at neutral pH gives a single complex for which a structure is proposed. The addition of calcium, indispensable for DTPA injection, leads to the appearance of a second bimetallic complex in very much smaller proportions than the first. The size distribution of some colloids was studied by ultrafiltration and permeation on gel. The preparation of colloidal rhenium sulphide and the technetium labelling conditions needed to obtain a very fine colloid were developed. The behaviour of technetium in the presence of colloidal rhenium sulphide and tin pyrophosphate was followed by sup(99m)Tc - sup(186)Re and sup(99m)Tc - sup(117m)Sn double-labelling tests. One reduced technetium fraction associates with the hydrolysed tin, the other follows the rhenium sulphide

  10. Effect of cyclophosphamide in blood cells biodistribution of 99 mTc-radiopharmaceuticals; Comparacao do efeito da ciclofosfamida na biodistribuicao sanguinea de radiofarmacos marcados com Tecnecio-99m

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Josefina S.; Braga, Ana C.S.; Gutfilen, Bianca; Bernardo-Filho, Mario [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Dept. de Biofisica e Biometria

    1997-12-01

    When a radiopharmaceutical is administered to a human being, a fraction of is always bound to the blood elements. Several authors have reported that therapeutic drugs can modify the binding of radiopharmaceutical to blood elements, this fact can alter its biodistribution. The aim of this work is to evaluate, in vivo, if the cyclophosphamide (CTX) is capable to alter the binding of {sup 99m} Tc-DTPA, {sup 99m} Tc-MDP, {sup 99m} Tc-GHA, {sup 99m} Tc-PHY and {sup 99m} Tc O{sub 4} to the blood elements.Mice were also treatment with CTX. After the radiopharmaceuticals were also administered to the same animals. Cyclophosphamide altered the percentage of radioactivity: in P for {sup 99m} Tc-PHY and (0,25 to 8 h) for {sup 99m} Tc-MDP; in FI-P (0,25 to 8 h) for {sup 99m} Tc-MDP; in FI-C (2 and 4 h) for {sup 99m} Tc-DTPA and (0,25 to 4 h) for {sup 99m} Tc O{sub 4}. 7 refs., 9 tabs.

  11. Comparison of the Efficiency for Tc-99m Tin-colloid and Tc-99m Phytate in Sentinel Node Detection in Breast Cancer Patients

    International Nuclear Information System (INIS)

    Lymphoscintigraphy and sentinel node biopsy has become a standard method for detection of axillary lymph node metastasis in breast cancer patients, but the standard radiopharmaceutical was not prepared. About detection of axillary lymph node metastasis by lymphoscintigraphy and sentinel node biopsy in breast cancer patient, we compared the results of Tc-99m Tin-colloid and Tc-99m Phytate by subareolar injection. This study included 382 breast cancer patients who were performed operation during 2001-2008. Three hundred forty nine patients was injected 0.8 ml of Tc-99m Tin-colloid (37-185 MBq) by subareolar injection. Thirty three patients was injected 0.8 ml of Tc-99m Phytate (37-185 MBq). Lymphoscintigraphy was performed in supine position and sentinel node localization was performed by hand-held gamma probe in operation. Among 349 patients by Tc-99m Tin-colloid, 312 cases (89.4%) localized the sentinel node by lymphoscintigraphy, 304 cases (87.1%) localized by gamma probe. Among 33 patients by Tc-99m Phytate, 32 cases (97.0%) localized by lymphoscintigraphy, 33 cases (100%) localized by gamma probe. Detection rate by lymphoscintigraphy and gamma probe was superior for Tc-99m Phytate compared to that with Tc-99m Tin-colloid, with a statistically significant difference. (p<0.05, p<0.05) Tc-99m Phytate is a better choice for localization of sentinel node than Tc-99m Tin-colloid in breast cancer patients

  12. Discordant uptake of Tc-99m DTPA-galactosyl human serum albumin and Tc-99m Sn colloid in a patient with severe acute hepatitis.

    Science.gov (United States)

    Miyazaki, C; Matsunaga, T; Kubo, K

    1994-08-01

    A patient with recently diagnosed severe acute hepatitis underwent serial liver scintigraphy with Tc-99m Sn colloid and Tc-99m DTPA-galactosyl human serum albumin. In initial studies, radionuclide distribution on Tc-99m DTPA-galactosyl human serum albumin scintigraphy was completely discrepant to that on Tc-99m Sn colloid scintigraphy. In a follow-up study 1 month later, the distribution of both radionuclides in the liver appeared relatively homogeneous. The uptake of Tc-99m DTPA-galactosyl human serum albumin and Tc-99m Sn colloid reflects the function of hepatocytes and Kupffer cells, respectively. Both kinds of scintigraphic study may be helpful to assess histopathologic change of different hepatic tissue architectures. PMID:7955747

  13. Cyclotron production of NCA 99mTc and 99Mo. An alternative non-reactor supply source of instant 99mTc and 99Mo → 99mTc generators

    International Nuclear Information System (INIS)

    The direct production of no-carrier-added (NCA) 6.02 h 99mTc and of 66 h 99mMo using proton beams on natural Mo targets was investigated. The major objective of this work was to evaluate the potential of utilizing high-intensity proton accelerators as a supply source of 99mTc and 99Mo for use in diagnostic nuclear medicine. The excitation functions for the production of the directly-made 99mTc via the 100Mo(p, 2n)99mTc reaction, and of its parent 99Mo via the 100Mo(p, pn) 99Mo and 100Mo(p, 2p)99mNb(15 s) → 99Mo reactions, were measured in the 68-8 MeV energy range. Single and cumulative yields for 99mTc and 99Mo, and for other Tc, Mo, Zr, Nb and Y radiocontaminants were also determined. The prospects of integrating the use of enriched 100Mo targets with high-intensity, dual beam, H- accelerators was analyzed. The potential of this combined method to replace or complement the current reactor-based supply sources of 99Mo → 99mTc generators, is also discussed. Finally, a brief analysis is made on the potential use of this combined technology to support the anticipated expansion of nuclear medicine in developing nations. (author)

  14. /sup 99m/Tc-aminohexylidendiphosphonate and /sup 99m/Tc-pyrophosphate in the scintigraphic diagnosis of experimental myocardial infarction in dogs

    Energy Technology Data Exchange (ETDEWEB)

    Duska, F.; Hadas, L.; Vizda, J.; Kafka, P.; Urbanova, E.; Palicka, V.; Mazurova, Y.; Kuba, M.; Grossman, V.

    1988-10-01

    /sup 99m/Tc-aminohexylidendiphosphonate (/sup 99m/Tc-AHDP) is a new Czechoslovak pharmaceutical of the phosphonate line which contains the amino group NH/sub 2/ in its molecule. This substance was tested in 5 dogs with experimentally provoked 48-h old myocardial infarction. The in-vivo scan and the radioactivity of tissue samples demonstrated that /sup 99m/Tc-AHDP is as suitable for imaging acute myocardial infarction as is the commonly used /sup 99m/Tc-pyrophosphate.

  15. Analysis of 99mTc-labeled radiopharmaceuticals by high-performance liquid chromatography

    International Nuclear Information System (INIS)

    High-performance liquid chromatography (HPLC) equiped with on line radiometric and optical detectors (i.e. radio-HPLC) have been applied to the radiochemical analysis of commonly-used 99mTc-radio pharma ceuticals with a view point to check the radiochemical purities of the compounds. Chromatographic conditions were determined by examination of the types of column, mobile phase and pH. An aqueous size-exclusion (Shim-pack Diol-300) and reversed-phase column (Zorbax-ODS) were found to be suitable for 99mTc-HSA and the other 99mTc-agents, respectively. The analysis of low molecular weight 99mTc-agents (e.g. 99mTc-DTPA, 99mTc-DMSA, 99mTc-pyrophosphate, 99mTc-phytic acid, 99mTc-MDS, 99mTc-HMDP) were done by reversed-phaseion pairing chromatography using a optimized mobile phase consisted on a mixture of 50 mM phosphate buffer (pH 7.0) and 2 mM TBA (tetra nbutyl) ammonium hydroxide) in 30 % methanol. The mobile phases for analysis of medium molecular weight 99mTc-HSA were consisted of a mixture of 50 mM phosphate buffer (ph 7.0) in 30 % methanol, and a mixtures of 1 % SDS (sodium dodecyl sulfonate) in Tris buffer (pH. 7.0), respectively. It was apparent from the radio-chromatograms obtained from these chromatographic conditions, that impurity of 99mTcO4 was observed in 99mTc-pyrophosphate, 99mTc-phytic acid, 99mTc-MDP, 99mTc-HMDP, and impurities of 99mTc-labeled species and 99mTcO4, were observed in 99mTc-HIDA, 99mTc-HIDA, 99mTc-HSA. The radiochemical impurities of the 99mTc-radiopharmaceuticals were ranged between 90 and 100 %. From these results, radio-HPLC has been shown to be suitable method for analysis of 99mTc-radiopharmaceuticals, with rapidity and excellent precision. (author)

  16. Mononuclear and dinuclear complexes of sup(99m)Tc

    Energy Technology Data Exchange (ETDEWEB)

    Yokoyama, A.; Horiuchi, K. (Kyoto Univ. (Japan). Faculty of Pharmaceutical Science)

    1982-10-01

    In the labelling of penicillamine (pen) with sup(99m)Tc, various complexes were detected. Studies of the conditions for differentiating the product formed in order to understand the chemical and biological behavior were carried out. Marked roles of pH and SnCl/sub 2/ concentration were observed. Controlled delivery of Sn/sup 2 +/ ion was obtained with the tin-adsorbed resin (Sn-resin) developed. Radiopharmaceutical and biological characterization of mononuclear and dinuclear complexes of Tc-Pen, under given sets of labeling conditions, were established. Those studies provided a good basis in technetium chemistry which facilitated the development of new Tc-radiopharmaceuticals. Selection of the appropriate ligand offered a stable mononuclear complex of Tc-bleomycin for tumor detection. Tc-Pen-ethyl-ester for blood-element labeling and, above all, development of a bis-thiosemicarbazone derivative as a new bifunctional chelating agent to be coupled to proteins, fatty acids, glucose etc. On the other hand, various cholescintigraphic agents were better conceived of as dinuclear complexes and this created the possibility of generating a new agent, a phthalein derivative, Tc-Pc containing the required coordination environment for the technetium to be bridged in a dinuclear state.

  17. Mononuclear and dinuclear complexes of /sup 99m/Tc

    Energy Technology Data Exchange (ETDEWEB)

    Yokoyama, A.; Horiuchi, K.

    1982-10-01

    In the labelling of penicillamine (Pen) with /sup 99m/Tc, various complexes were detected. Studies of the conditions for differentiating the product formed in order to understand the chemical and biological behavior were carried out. Marked roles of pH and SnCl/sub 2/ concentration were observed. Controlled delivery of Sn/sub 2//sup +/ ion was obtained with the tin-adsorbed resin (Sn-resin) developed. Radiopharmaceutical and biological characterization of mononuclear and dinuclear complexes of Tc-Pen, under given sets of labelling condition, were established. Those studies provided a good basis in technetium chemistry which facilitated the development of new Tc-radiopharmaceuticals. Selection of the appropriate ligand offered a stable mononuclear complex of Tc-bleomycin for tumor detection, Tc-Pen-ethyl-ester for blood-element labeling and, above all, development of a bis-thiosemicarbazone derivative as a new bifunctional chelating agent to be coupled to proteins, fatty acids, glucose etc. On the other hand, various cholescintigraphic agents were better conceived of as dinuclear complexes and this created the possibility of generating a new agent, a phthalein derivative, Tc-PC, containing the required coordination environment for the technetium to be bridged in a dinuclear state.

  18. Dynamic 99mTc-DTPA radioaerosol lung scanning

    International Nuclear Information System (INIS)

    51 patients (10 normal subjects and 41 pathological ones) underwent dynamic 99mTc-DTPA radioaerosol lung scanning to evaluate different technical aspects of alveolar-capillary permeability, as reflected by the rate constant (k) of the radiotracer exponential washout. A significant difference (p<0.01) was found between normal and pathological subjects but not among pathological groups as a damage of alveolar-capillary barrier is a non specific event common to different lung conditions. No significant correlation was found between total lung k and either spirometric tests (FEV1 and MEF75) or radioaerosol initial distribution, proving thus that the alveolar-capillary permeability is not related to derangements of ventilation. Inter and intraoperator reproducibilities were fairly satisfactory, showing correlation coefficients of 0.992 and 0.996, respectively. Sensitivity and specificity for the diagnosis of increased alveolar-capillary permeability were calculated using different criteria. The best results in the patients studied were achieved by using total rather than regional ks, leading to a specificity of 90% and a sensitivity of 86%. (orig.)

  19. Tc 99m - scorpion venom: labelling, biodistribution and scintiimaging

    International Nuclear Information System (INIS)

    Labelling of scorpion (Mesobuthus tamulus concanesis Pocock) venom was successfully achieved with Tc 99m using direct tin reduction procedure. Biodistribution studies were carried out in Wistar rats at different time intervals after i.v. administration of the labelled venom. Scintiimages were obtained after scorpion envenoming using a large field of view gamma camera to ascertain the pharmacological action of venom in the body. Within 5 min of administration, labelled venom was found in the blood (27.7%), muscle (30.11%), bone (13.3%), kidneys (11.5%), liver (10.4%) and other organs. The level of venom in the kidneys was higher than in the liver. The labelled venom was excreted through renal and hepatobiliary pathways. An immunoreactivity study was carried out in rabbits after i.v. injection of labelled scorpion venom followed by the injection of the species specific antivenom. A threefold increase in uptake by the kidneys ss was observed compared with that seen with scorpion venom alone. the neutralisation of the venom in the kidneys was higher than in the liver. (author)

  20. Tc 99m - scorpion venom: labelling, biodistribution and scintiimaging

    Energy Technology Data Exchange (ETDEWEB)

    Murugesan, S.; Noronha, O.P.D.; Samuel, A.M. [Bhabha Atomic Research Centre, Mumbai (India). Tata Hospital Annexe. Radiation Medicine Center; Murthy, K. Radha Krishna [Seth G.S. Medical College, Mumbai (India). Dept. of Physiology

    1999-07-01

    Labelling of scorpion (Mesobuthus tamulus concanesis Pocock) venom was successfully achieved with Tc 99m using direct tin reduction procedure. Biodistribution studies were carried out in Wistar rats at different time intervals after i.v. administration of the labelled venom. Scintiimages were obtained after scorpion envenoming using a large field of view gamma camera to ascertain the pharmacological action of venom in the body. Within 5 min of administration, labelled venom was found in the blood (27.7%), muscle (30.11%), bone (13.3%), kidneys (11.5%), liver (10.4%) and other organs. The level of venom in the kidneys was higher than in the liver. The labelled venom was excreted through renal and hepatobiliary pathways. An immunoreactivity study was carried out in rabbits after i.v. injection of labelled scorpion venom followed by the injection of the species specific antivenom. A threefold increase in uptake by the kidneys ss was observed compared with that seen with scorpion venom alone. the neutralisation of the venom in the kidneys was higher than in the liver. (author)

  1. Clinical evaluation of 99mTc-pseudogas (Technegas)

    International Nuclear Information System (INIS)

    99mTc-pseudogas (Technegas)--a new agent for lung ventilation scintigraphy--was studied in 6 healthy controls and 42 patients with various pulmonary diseases. Technegas easily enabled the obtaining of multiple planar images and even SPECT images. Its image was superior to that of 133Xe gas or 81mKr gas. We classified its posterior deposition patterns into 6 patterns (Type A: normal distribution; Type B1: mildly irregular distribution; Type B2 : severely irregular distribution; Type C: severely irregular distribution mingled with hot spots; Type D: non hilar hot spots; and Type E: hilar hot spots). The deposition patterns showed mainly Type B1 in restrictive pulmonary diseases and Type B2, C and D in obstructive pulmonary diseases. Further, the deposition patterns correlated well with %FEV1.0, which was a good indicator of the severity of both obstructive and restrictive pulmonary diseases. No adverse reaction was found. We concluded that Technegas is a safe and very useful agent for ventilation study. (author)

  2. Uptake and localization of sup(99m)technetium-methylene-diphosphonate in bone

    International Nuclear Information System (INIS)

    The author investigated the uptake and localization of 99m-technetium-methylene-diphosphonate (99m-Tc-MDP) in bone, to develop a sensitive mean for the detection of early osseous disease. In an electrolysis procedure without the presence of contaminating reductants a 99m-Tc-MDP complex is formed with clear bone-seeking properties. The scans performed in experimental animals are comparable in quality with 99m-Tc(Sn)-MDP scans. The uptake of 99m-Tc-MDP is faster and higher than the uptake of reduced hydrolyzed 99m-Tc. Uptake of 99m-Tc(Sn)-MDP in bone can only take place after decomposition of the complex. As 99m-Tc-MDP is taken up as a unit, this may be a better agent to evaluate the osteoblastic activity in the skeleton. (Auth./R.B.)

  3. Early myocardial damage assessment in dystrophinopathies using 99Tcm-MIBI gated myocardial perfusion imaging

    Directory of Open Access Journals (Sweden)

    Zhang L

    2015-12-01

    Full Text Available Li Zhang,1,* Zhe Liu,2,* Ke-You Hu,3 Qing-Bao Tian,3 Ling-Ge Wei,4 Zhe Zhao,5 Hong-Rui Shen,5 Jing Hu5 1Department of Cardiovascular Disorders, 2Department of Geriatrics, The Third Hospital of Hebei Medical University, 3The Public Health Department, Hebei Medical University, 4Department of Nuclear Medicine, 5Department of Neuromuscular Disorders, The Third Hospital of Hebei Medical University, Shijiazhuang, People’s Republic of China *Li Zhang and Zhe Liu are first coauthors of this paper Background: Early detection of muscular dystrophy (MD-associated cardiomyopathy is important because early medical treatment may slow cardiac remodeling and attenuate symptoms of cardiac dysfunction; however, no sensitive and standard diagnostic method for MD at an earlier stage has been well-recognized. Thus, the aim of this study was to test the early diagnostic value of technetium 99m-methoxyisobutylisonitrile (99Tcm-MIBI gated myocardial perfusion imaging (G-MPI for MD.Methods and results: Ninety-one patients underwent 99Tcm-MIBI G-MPI examinations when they were diagnosed with Duchenne muscular dystrophy (DMD (n=77 or Becker muscular dystrophy (BMD; n=14. 99Tcm-MIBI G-MPI examinations were repeated in 43 DMD patients who received steroid treatments for 2 years as a follow-up examination. Myocardial defects were observed in nearly every segment of the left ventricular wall in both DMD and BMD patients compared with controls, especially in the inferior walls and the apices by using 99Tcm-MIBI G-MPI. Cardiac wall movement impairment significantly correlated with age in the DMD and BMD groups (rs=0.534 [P<0.05] and rs=0.784 [P<0.05], respectively. Intermittent intravenous doses of glucocorticoids and continuation with oral steroid treatments significantly improved myocardial function in DMD patients (P<0.05, but not in BMD patients.Conclusion: 99Tcm-MIBI G-MPI is a sensitive and safe approach for early evaluation of cardiomyopathy in patients with DMD or BMD

  4. Comparative myocardial uptake of technetium-99 m sestamibi and technetium-99m tetrofosmin one hour after stress injection

    International Nuclear Information System (INIS)

    Technetium-99m sestamibi and 99mTc-tetrofosmin are at present the preferred tracers for simultaneous assessment of myocardial perfusion and function by gated single-photon emission tomography (SPET). The aim of this work was to compare sestamibi and tetrofosmin myocardial uptake 1 h after stress injection. Consecutive unselected patients were studied either with sestamibi or with tetrofosmin on a random basis, until at least 100 patients had been enrolled for each gender and tracer. Stress was obtained by dipyridamole or exercise or combined dipyridamole + exercise; in the latter cases, exercise was sustained for at least 1.5 min after tracer injection. Injected activity was similarly adjusted to body weight. For each patient, imaging began 60-75 min after injection. All SPET projections were summed; due to the acquisition technology (''roving zoom'', i.e. a mobile zoom), the heart always appeared at the centre of the frame in all projections and in the sum image. Thus minimal lung background contamination could be assumed in an elliptic region of interest placed over the heart on the sum image. Three indexes were analysed: total myocardial counts (Sum), mean myocardial pixel (Mean) and maximum myocardial pixel (Max). Four patient groups were analysed: males with sestamibi or tetrofosmin (MS: n = 189 and MT: n = 157), females with sestamibi or tetrofosmin (FS: n = 101 and FT: n = 104). MS and MT groups were comparable for physical variables, maximum heart rate and stress type, as were the FS and FT groups. Sum, Mean and Max were significantly higher with sestamibi (P = 0.0001 by ANOVA). Comparing MS vs MT and FS vs FT, mean values ± SD were as follows: for Sum (kcounts) 750±184 vs 652±166, and 707±202 vs 594±189; for Mean (counts) 4517±1171 vs 4107±898, and 4908±1119 vs 4144±1025; and for Max (counts) 6471±1654 vs 5794±1312, and 7318±1886 vs 6152±1684. The mean gain with sestamibi was +15%, +10% and +12% in males, and +19%, +18% and +19% in females

  5. The quality control of technetium-99m radiopharmaceuticals produced at the AAEC Research Establishment

    International Nuclear Information System (INIS)

    The methods of quality control used for technetium-99m radiopharmaceuticals produced at the AAEC Research Establishment are described for both non-fission and fission derived sources of sodium pertechnetate, technetium-99m labelled radipopharmaceuticals, and reagent kits produced for technetium-99m labelling

  6. Patient dosimetry of intravenously administered 99mTc-annexin V

    NARCIS (Netherlands)

    Kemerink, G J; Liem, I H; Hofstra, L; Boersma, H H; Buijs, W C; Reutelingsperger, C P; Heidendal, G A

    2001-01-01

    UNLABELLED: Annexin V labeled with 99mTc is evaluated as a potential in vivo marker for tissue with increased apoptosis. Promising results in patients have been obtained with 99mTc-(n-1-imino-4-mercaptobutyl)-annexin V (99mTc-i-AnxV). Because information on biodistribution and radiation burden is de

  7. Combined protocol of 99mTc-Ciprofloxalina and 99mTc-MDP in the diagnosis of complicated osteomyelitis and infected prosthesis

    International Nuclear Information System (INIS)

    Introduction: Bone scintigraphy with 99mTc-MDP is a useful technique in the diagnosis of osteomyelitis, however, has limited specificity in the presence of previous bone pathology (complicated osteomyelitis). 99mTc-ciprofloxacin is one of the most widely used radiotracers for the detection of bone infection, although controversies persist on its diagnostic performance. Objective: To determine the clinical value of 99mTc-ciprofloxacin/99mTc-MDP combined protocol in the diagnosis of complicated osteomyelitis and infected joint prosthesis. Materials and methods: 37 patients with clinically suspected complicated osteomyelitis or infected prosthesis were studied with 99mTc-ciprofloxacin and 99mTc-MDP scintigraphy. 26/37 patients had previous fractures, 7 had knee replacements and 4 had hip replacements. All of the patients underwent clinical and bacteriological follow-up. Results: The method presented sensitivity of 94%, 79% specificity, 81% positive predictive value and 94% negative predictive value, with an accuracy of 86%. Conclusions: The combined protocol using 99mTc-ciprofloxacin/99mTc-MDP showed high diagnostic performance in complicated osteomyelitis and infected joint prosthesis

  8. The influence of experimental conditions on the preparation of 99mTc-pyrrolidino-methyl tetracycline [99mTc-PMT] in the form of an instant kit

    International Nuclear Information System (INIS)

    The effects of varying the experimental conditions of the preparation of the renal scanning agent 99mTc-PMT on its organ distribution have been investigated. The stability of 99mTc-PMT was determined in vitro. A procedure for the preparation of this agent is recommended. (orig.)

  9. Sup (99m) Tc-MDP accumulation and absence of concentration of sup (99m) Tc-sulfur colloid in the spleen

    International Nuclear Information System (INIS)

    A case of a nine years old girl with sickle cell anemia, that showed splenic accumulation of sup (99m) Tc-MDP during bone scintigraphy is reported. On the other side, the scan with sup (99m) Tc-sulfur colloid showed no uptake in the spleen. These findings are discussed with a brief review of the pertinent literature. (Author)

  10. (99m)Tc-Galacto-RGD2: a novel 99mTc-labeled cyclic RGD peptide dimer useful for tumor imaging.

    Science.gov (United States)

    Ji, Shundong; Czerwinski, Andrzej; Zhou, Yang; Shao, Guoqiang; Valenzuela, Francisco; Sowiński, Paweł; Chauhan, Satendra; Pennington, Michael; Liu, Shuang

    2013-09-01

    This study sought to evaluate [(99m)Tc(HYNIC-Galacto-RGD2)(tricine)(TPPTS)] ((99m)Tc-Galacto-RGD2: HYNIC = 6-hydrazinonicotinyl; Galacto-RGD2 = Glu[cyclo[Arg-Gly-Asp-D-Phe-Lys(SAA-PEG2-(1,2,3-triazole)-1-yl-4-methylamide)

  11. Noninvasive quantitation of myocardial infarction with technetium-99m pyrophosphate

    International Nuclear Information System (INIS)

    We sought to quantitate infarct size using radioactive imaging techniques. Infarcts were created in closed chest dogs. Using a scintillation camera interfaced to a computer, infarct images were made in the anterior, left lateral, LAO, and RAO projections, 48 hours after infarction and 75 to 90 min following the intravenous injection of 15 mCi of technetium-99m pyrophosphate (Tc-PYP). Images were computer enhanced and area was calibrated with a radioactive grid source of known dimensions. Image radioactivity was normalized for decay and dose corrected for body weight. Animals were sacrificed two hours following the injection of Tc-PYP. Postmortem images were also computer enhanced and calibrated. Gross infarct area and weight were estimated and transmural biopsies were evaluated for Tc-PYP activity and analyzed for creatine phosphokinase (CPK) content. Contiguous biopsies were pathologically analyzed and graded. There was a negative correlation between tissue Tc-PYP activity and CPK content (r = -0.89). Pathologic severity worsened with increased Tc-PYP activity and diminished CPK content. There was a good correlation between gross infarct area and image infarct area, both in vivo (r = 0.79), and at post-mortem examination (r = 0.95). Gross infarct weight also correlated well with image infarct activity in vivo (r = 0.83 in the RAO view) and at postmortem examination (r = 0.87). An additional correlation between gross infarct weight and in vivo image infarct area (r = 0.92 in the LAO view) appeared most promising for future clinical evaluation. These experimental relationships are analyzed and future patient application of these imaging techniques are considered

  12. Technetium-99m-sestamibi scintigraphy in breast nodes; Estudo cintilografico de nodulos mamarios com tecnecio-99m-sestamibi

    Energy Technology Data Exchange (ETDEWEB)

    Pellini, Marcos Pinto; Fonseca, Lea Mirian Barbosa da [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina; Balen, Jacir Luiz; Fabricio, Maria Ines Menescal [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina. Inst. de Ginecologia; Amarante Junior, Jose Luiz de Medeiros

    1997-01-01

    The purpose was to was to determine if technetium-99m-sestamibi accumulates preferentially within the malignant palpable nodes of breast. Twenty-five patients, mean age 36.16 ({+-} 9.34) year, and without any other additional information, underwent breast scintigraphy and excisional biopsy. We had nineteen true-negative cases, four true-positive, two false-positive and none false-negative. Sensitivity, 100% specificity, 90.5%, accuracy, 92%; PPV, 66.7%, NPV, 100%. The four true positive were invasive intraductal carcinomas and one of them metastases in auxiliary lymph-nodes, confirmed in biopsy and well defined in scintigraphy. The two false-positives were a fibroadenoma with high cellularity and a fibrodisplasy surrounded by chronic inflammatory process. Two statistical tests were applied: chi-square and Fisher. Both rejected the antithesis at a confidence interval of 99% (p , 0.01) We concluded that technetium-99-sestamibi accumulates preferentially within the malignant nodes of breast. (author) 17 refs., 3 figs., 2 tabs.

  13. /sup 99m/Tc-aminohexylidendiphosphonate and /sup 99/mTc-Pyrophosphate in the scintigraphic diagnosis of experimental cardiomyopathy in dogs

    Energy Technology Data Exchange (ETDEWEB)

    Duska, F.; Kafka, P.; Mazurova, Y.; Hadas, L.; Vizda, J.; Palicka, V.; Grossman, V.

    1987-10-01

    Experimental cardiomyopathy was provoked in 24 dogs with high intravenous doses of adrenaline and theophylline. These lesions were studied by means of the new agent /sup 99m/Tc-AHDP and /sup 99m/Tc-PYP in comparison. Cardiomyopathy could be imaged as early as 4 h after the onset of involvement but not later than 7 days. A maximum accumulation occurred in lesions 24 h old, /sup 99m/Tc uptake in the myocardium was graded scintigraphically, /sup 99m/Tc-AHDP was accumulated in the altered myocardium to a greater extent than /sup 99m/Tc-PYP. Scintigraphic findings were in good agreement with plasma levels of creatine-kinase. A comparison with histology demonstrated that the maximum accumulation of radiopharmaceuticals occurred at the time when the development of myocardium involvement reached the stage of myocytolysis.

  14. Pulmonary clearance of 99mTc-DTPA and 99mTc-albumin in rabbits with surfactant dysfunction and lung injury

    International Nuclear Information System (INIS)

    We measured the pulmonary clearance of inhaled 99mTc-DTPA and 99mTc-albumin in rabbits with surfacant dysfunction induced by dioctyl sodium sulphosuccinate and in rabbits with lung injury induced by oleic acid. After inhalation of 99mTc-albumin in ten animals, clearance of the tracer from the lungs was monitored for 90 min. The first 30 min was a control period. Dioctyl sodium sulphosuccinate was then administered in aerosol and after another 30 min oleic acid was injected intravenously. Ten other rabbits were given 99mTc-DTPA, and clearance was externally recorded for 60 min. Five animals inhaled detergent aerosol and five animals were given oleic acid intravenously after 30 min. Airway pressures, tidal volume, and arterial blood gases were measured before and after each intervention. The half-life of 99mTc-albumin in the lung was 442 ± 123 min during the control period, 363 ± 52 min after detergent administration, and 134 ± 18 min after oleic acid administration. The half-life of 99mTc-DTPA was 94 ± 16 min before and 10 ± 0.6 min after detergent administration and 75 ± 12 min before and 18 ± 1.8 min after oleic acid administration. Gas exchange was not affected by administration of dioctyl sodium sulphosuccinate but markedly impaired after injection of oleic acid. Compliance of the respiratory system remained unaffected by detergent but decreased after injection of oleic acid. The results indicate that the rate limiting factors for the alveolo-capillary transfer of 99mTc-albumin and 99mTc-DTPA are different. Surfactant dysfunction affects the transfer of 99mTc-DTPA but not 99mTc-albumin. (author)

  15. Application of extraction generator for preparation of radiopharmaceuticals on 99mTc base

    International Nuclear Information System (INIS)

    Data on extraction generator use for preparation of high-purity 99mTc of 99Mo are presented, quality of 99mTc solution is investigated. Extractant (methyl ethyl ketone) radiolysis products are studied. High degree of purification of 99mTc eluate from extraction generator both of organic and inorganic impurities is achieved. This fact is a guarantee of high quality of radiopharmaceuticals prepared using this 99mTc solution. 99mTc solution obtained from extraction generator surpasses by quality analogues solution from sorption generator. In the case of use of centrifugal extractor for 99Mo and 99mTc separation concentration of methyl ethyl ketone in 99mTc solution is not more 0.04 mg/ml

  16. Technetium-99m sestamibi kinetics in reperfused canine myocardium

    International Nuclear Information System (INIS)

    The purpose of the current study was to clarify the myocardial kinetics of technetium-99m sestamibi when the latter is administered during reperfusion. Sestamibi has in the past been given to patients following thrombolytic therapy to document reperfusion and assess salvage. However, the factors which affect sestamibi kinetics during reperfusion are not clearly defined. In this study the left circumflex coronary artery was occluded for 2 h in six dogs (group 1) and for 3 h in six dogs (group 2), followed by reperfusion. Five additional dogs were not reperfused (group 3). Sestamibi was administered during reperfusion in groups 1 and 2, and during ongoing occlusion in group 3. Regional myocardial sestamibi activity was monitored for 3 h using miniature implanted radiation detectors and gamma camera imaging. Group 1 dogs had no infarcts, group 2 had moderate infarcts (mean: 13.9%), and group 3 had large infarcts (mean: 25.2%). Three-hour fractional myocardial clearances were significantly greater for reperfused infarcted (group 2) (0.23±0.02 SEM) and for nonreperfused infarcted myocardium (group 3) (0.24±0.02) compared to control (0.10±0.01) and reperfused noninfarcted myocardium (group 1) (0.07±0.02; P<0.01). Quantitative image analysis demonstrated a significant reduction in the left circumflex/left anterior descending count ratios from initial to final scans for group 2 (0.74±0.03 to 0.65±0.03, P<0.05), and a trend towards a reduction in the count ratios from initial to final scans for group 3 (0.38±0.04 to 0.30±0.04; P=0.06). Thus, probederived myocardial sestamibi kinetics following reperfusion do differentiate non-infarcted from infarcted myocardium. Although the detection of accelerated clearance can be demonstrated by quantitative analysis of gamma camera images in dogs, this may be technically difficult in clinical situations. (orig.)

  17. Investigation of four {sup 99m}Tc-labeled bacteriophages for infection-specific imaging

    Energy Technology Data Exchange (ETDEWEB)

    Rusckowski, Mary [Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655-0243 (United States)], E-mail: mary.rusckowski@umassmed.edu; Gupta, Suresh; Liu Guozheng; Dou Shuping; Hnatowich, Donald J. [Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655-0243 (United States)

    2008-05-15

    Introduction: This study investigated radiolabeled bacteriophages for specific detection of infection through gamma imaging. Previously, a {sup 99m}Tc-labeled M13 phage demonstrated specific binding for its host Escherichia coli in vitro and in mice through imaging. Methods: This study was extended to phages P22, E79, VD-13 and phage 60. Each was radiolabeled with {sup 99m}Tc using the chelator MAG{sub 3}, and were evaluated for binding to host and non-host bacteria in vitro and in a mouse infection model. Results: In vitro, each {sup 99m}Tc-phage bound to its host at least 4-fold higher than to non-host bacteria. For example, {sup 99m}Tc-E79 showed 10- to 20-fold greater binding to host Pseudomonas aeruginosa compared to non-host Escherichia coli and Salmonella enterica, and {sup 99m}Tc-phage 60 showed 20-fold greater binding to host Klebsiella pneumoniae over non-hosts. Mice received host or non-host bacteria in one thigh, and 3 h later, the {sup 99m}Tc-phages were administered intravenously. After a further 3 h, the tissues were counted. Liver accumulation was highest for {sup 99m}Tc-E79, averaging 39% compared to an average of 13% for the other {sup 99m}Tc-phages. Animals infected with host bacteria showed infected thigh/normal thigh ratios of 14.2 for {sup 99m}Tc-E79, 2.9 for {sup 99m}Tc-P22, 3.5 for {sup 99m}Tc-VD-13 and 2.1 for {sup 99m}Tc-phage 60. Conclusions: Although specific host binding was observed in vitro for each of these four {sup 99m}Tc-phages, only {sup 99m}Tc-E79 showed specificity for its host in an in vivo model.

  18. Assessment of the direct cyclotron production of (99m)Tc: An approach to crisis management of (99m)Tc shortage.

    Science.gov (United States)

    Rovais, Mohammad Reza Aboudzadeh; Aardaneh, Khosro; Aslani, Gholamreza; Rahiminejad, Ali; Yousefi, Kamran; Boulouri, Fatemeh

    2016-06-01

    Nowadays, the cyclotron production of technetium-99m ((99m)Tc) has been increased, due to the worldwide (99m)Tc generator shortage. In the present work, an improved strategy for the production of (99m)Tc, using the proton irradiation of the enriched (100)Mo was developed. The performance of this method in terms of the production yield, chemical purity, radiochemical purity, as well as radionuclide purity was evaluated. The average production yield was measured to be 356MBqμA(-1)h(-1). A good agreement was found between the calculated production yield and the experimental one. The radiochemical separation and total recovery yields of (99m)Tc were 92% and 69%, respectively. The radiochemical and the radionuclide purities of the (99m)Tc were 99% and >99.99% at the end of purification, respectively. The results of quality control tests (QC) support the concept that cyclotron-produced (99m)Tc is suitable for preparation of USP-compliant.

  19. Regional differences between 99mTc-ECD and 99mTc-HMPAO SPET in perfusion changes with age and gender in healthy adults

    International Nuclear Information System (INIS)

    A number of studies using single-photon emission tomography (SPET) have shown perfusion changes with age in several cortical and subcortical areas, which might distort the results of perfusion imaging studies of neuropsychiatric disorders. Technetium-99m labelled ethyl cysteinate dimer (ECD) and hexamethylpropylene amine oxime (HMPAO) are both used as markers of cerebral perfusion, but have different pharmacokinetics and retention patterns. The aim of this study was to determine whether age and gender effects on perfusion SPET differ depending on whether 99mTc-HMPAO or 99mTc-ECD is used. Forty-five subjects (20 male and 25 female, mean age 52.8±6.6 years) were assigned to 99mTc-HMPAO SPET (HMPAO group), and 39 subjects (24 male and 15 female, mean age 52.6±6.7 years) to 99mTc-ECD SPET (ECD group). SPET images were obtained about 10 min after intravenous injection of approximately 800 MBq 99mTc-HMPAO or 99mTc-ECD using the same SPET scanner. Three-dimensional volumetric magnetic resonance imaging was performed to as7sess morphological changes in the grey matter. All image processing and statistical analyses were performed using SPM99 software. An area in the right anterior frontal lobe showed an increase in perfusion with age only in the HMPAO group, whereas areas in the bilateral retrosplenial cortex showed decreases in perfusion with age only in the ECD group; neither group showed corresponding changes in the grey matter. The present study shows that different effects of age on perfusion are observed depending on whether 99mTc-HMPAO and 99mTc-ECD is used. This suggests that the results of perfusion SPET are differently confounded depending on the tracer used, and that perfusion SPET with these tracers has limitations when used in research on subtle perfusion changes. (orig.)

  20. [Quantification of separate renal function using Tc 99m DTPA and Tc 99m DMSA. Correlations between individual isotopic data and creatinine clearance].

    Science.gov (United States)

    Chevet, D; Moisan, A; Le Pogamp, P; Le Cloirec, J; Wehbe, B; Herry, J Y

    1984-01-01

    The purpose of this prospective study (30 controls and 100 renal patients) is to compare one to each other the values of renal function measured with: 1--the DTPA 99mTc , 2--the DMSA 99mTc , and 3--the creatinine clearance. After the control values being established, correlations are done for the whole group (n = 130) between the creatinine clearance, the DTPA 99mTc clearance and the fixation rate of the DMSA 99mTc . The correlations obtained are:--Clcr Versus Cldtpa = 0,91.--Clcr Versus % Dmsa = 0,90. - Cldtpa Versus % Dmsa = 0,93. and indicate the two isotopic tests performed give similar quantitative results than creatinine clearance. Camera and computer allow to measure the separate renal function one by one, the two isotopic test giving similar values for each kidney. The results of these studies give a good concordance with the observed diseases, except for partial renal artery stenosis. The pyelic retention of DMSA 99mTc product, in condition of ureteral compression, gives a potential overestimation of this test and is a matter to debate. The extreme simplicity and facility of DMSA 99mTc procedures make this investigation accessible to any patient and give excellent morphological and functional tests. DTPA 99mTc clearances--global and separate kidney--give a more sensitive approach than DMSA 99mTc does because vascular captation , elimination rates could be focused. DTPA 99mTc procedure is to be used in patients with transplanted kidney, silent IVP kidney, contrast media contraindication or for exploring glomerular clearance without urine collection.

  1. Mechanism of sup(99m)Tc-methylene diphosphonate (sup(99m)Tc-MDP) localization in experimental studies of bone tumors

    International Nuclear Information System (INIS)

    [Materials and Methods] (I) VX2 carcinoma was transplanted to the rabbit tibia, sup(99m)Tc-MDP was intravenously injected when bone destruction was radiologically seen. Preoperative scintigraphs, postoperative one of the resected tibia and fibra and scintigraphs of sliced tumor were compared with each other. Tissue fragments were collected from the sliced tumor, and RI was measured to compare concentration rates at different parts together with histological investigations. (II) Tetracycline was given to the rabbit of Experiment (I) to compare with the concentration of sup(99m)Tc-MDP. (III) Microautoradiography method was done on the experimental animals for investigation of the concentration of sup(99m)Tc-MDP. [Results] (I) Localization of sup(99m)Tc-MDP was increased at the sites of tumorous bone formation, of reactive bone formation at tumor edges, and of cartilage calcification, with little concentration at tumor cells or necrotic tissue. (II) Deposition sites of tetracycline nearly corresponded with those showing higher concentration of sup(99m)Tc-MDP. (III) microautoradiograms showed high precipitation of silver granules at new bone formation sites. More remarkable localization of sup(99m)Tc-MDP were seen at sites of formation of tumorous bone, of reactive bone at tumor edges and of cartilage calcification. The results deny the view that localization of sup(99m)Tc-MDP was determined by increased blood flow and collagen metabolism, and affirm the view of Galasko et al. that it was determined by new bone formation. Further, results of studies on tetracycline distribution correspond with those of investigations on sup(99m)Tc-MDP localization, indicating that sup(99m)Tc-MDP concentration is strongly determined by tumorous and reactive new bone formation and in cartilage calcification. (author)

  2. Differential uptake of Tc-99m DMSA and Tc-99m EC in renal tubular disorders: Report of two cases and review of the literature

    International Nuclear Information System (INIS)

    Tc-99m DMSA and Tc-99m EC studies are invaluable functional imaging modalities for renal structural and functional assessment. Normally, the relative renal function estimated by the two methods correlates well with each other. We here present two patients with renal tubular acidosis who showed impaired/altered DMSA uptake with normal EC renal dynamic study depicting the pitfall of DMSA imaging in tubular disorders. The two presented cases also depict distinct pattern of Tc-99m DMSA scintigraphic findings in patients with proximal and distal renal tubular acidosis, thus highlighting the factors affecting DMSA kinetics

  3. Is the renal uptake of 99mTc-DMSA decreased in microalbuminuric diabetic patient?

    International Nuclear Information System (INIS)

    Diabetic nephropathy is the most common cause of end stage renal disease and the incidence is progressively increasing. The aim of this study was to investigate the differences of 99mTc-DMSA renal uptake among diabetic patients with normoalbuminuria, microalbuminuria and overt proteinuria, and then to determine the clinical usefulness of 99mTc-DMSA in predicting early diabetic nephropathy. 99mTc-DMSA scan was performed and a total renal uptake of 99mTc-DMSA was measured in 145 diabetic patients. Patients were divided into 3 groups according to the amount of 24 hour urinary albumin excretion as Group I (normoalbuminuria, 74 cases ), Group II (microalbuminuria, 39 cases), and Group III (overt proteinuria, 32 cases). The differences of 99mTc-DMSA renal uptake among the 3 groups and the correlation between the renal uptake of 99mTc-DMSA and other clinical parameters were analyzed. The total renal uptake of 99mTc-DMSA of Group II (40.8±11.0%) was significantly lower than that of Group I (54.4±6.3%, p99mTc-DMSA total renal uptakes correlated negatively with serum creatinine level (r=0.629, p99mTc-DMSA total renal uptake of diabetic patients with microalbuminuria was significantly decreased compared with that of patients of normoalbuminuria. Therefore, 99mTc-DMSA scan can be used as a diagnostic study for early detection of the diabetic nephropathy

  4. [sup 99m]Tc-ECD SPECT study in dementia and aphasia

    Energy Technology Data Exchange (ETDEWEB)

    Kuwabara, Yasuo; Ichiya, Yuichi; Otsuka, Makoto; Sasaki, Masayuki; Akashi, Yuko; Fukumura, Toshimitsu; Yoshida, Tsuyoshi; Masuda, Kouji; Ichimiya, Atsushi (Kyushu Univ., Fukuoka (Japan). Faculty of Medicine)

    1993-09-01

    We studied clinical significance of [sup 99m]Tc-L,L,-ethyl cysteine dimer ([sup 99m]Tc-ECD) SPECT study in dementia and aphasia, and compared it with [sup 99m]Tc-HMPAO SPECT study. The subjects consisted of 13 patients, including 10 patients with dementia and 3 patients with aphasia. Hypoperfusion areas were detected in 5 out of 10 patients with dementia and 2 out of 3 patients with aphasia in [sup 99m]Tc-ECD SPECT, and in 4 out of 10 patients with dementia and all of 3 patients with aphasia in [sup 99m]Tc-HMPAO SPECT. The count rate ratios in [sup 99m]Tc-ECD and [sup 99m]Tc-HMPAO SPECT were correlated well with each other, and the contrast of the [sup 99m]Tc-ECD SPECT image was equivalent or slightly higher as compared with [sup 99m]Tc-HMPAO. Therefore, [sup 99m]Tc-ECD SPECT study was considered to be useful for the evaluation of cerebral perfusion in dementia and aphasia. (author).

  5. 99mTc-ECD SPECT study in dementia and aphasia

    International Nuclear Information System (INIS)

    We studied clinical significance of 99mTc-L,L,-ethyl cysteine dimer (99mTc-ECD) SPECT study in dementia and aphasia, and compared it with 99mTc-HMPAO SPECT study. The subjects consisted of 13 patients, including 10 patients with dementia and 3 patients with aphasia. Hypoperfusion areas were detected in 5 out of 10 patients with dementia and 2 out of 3 patients with aphasia in 99mTc-ECD SPECT, and in 4 out of 10 patients with dementia and all of 3 patients with aphasia in 99mTc-HMPAO SPECT. The count rate ratios in 99mTc-ECD and 99mTc-HMPAO SPECT were correlated well with each other, and the contrast of the 99mTc-ECD SPECT image was equivalent or slightly higher as compared with 99mTc-HMPAO. Therefore, 99mTc-ECD SPECT study was considered to be useful for the evaluation of cerebral perfusion in dementia and aphasia. (author)

  6. Hydroxamamide as a chelating moiety for the preparation of 99mTc-radiopharmaceuticals. Pt. 3: Characterization of various 99mTc-hydroxamamides

    International Nuclear Information System (INIS)

    Five hydroxamamide (Ham) derivatives with various nonpolar substituents and different lipophilicities have been selected as ligands for preparation of 99mTc-complexes. Analysis via high performance liquid chromatography (HPLC) resolved the 99mTc-complexes of each ligand into two radioactive components, which are considered as isomers. The stability of both components was studied at pH 5 and 7, showing good stability at pH 7, but some transition between the components at pH 5. Use of mixture of two ligands for complexation showed not only the complexes of the separate ligands, but also complexes containing both ligands. These results indicate that hydroxamamides very likely form complexes composed of Ham and 99mTc in a 2:1 molar ratio. This may also explain the stability of the 99mTc-Ham complexes in spite of the bidentate character of a Ham ligand. (author)

  7. Guarana (Paullinia cupana) effect on radiolabelling of erythrocytes with Technetium-99m; Efeito do guarana (Paulinnia cupana) na marcacao de hemaceas com tecnecio-99m

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Joelma F.; Braga, Ana Cristina S.; Avila, Antonio Sergio R.; Gutfilen, Bianca [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Biologia; Bernardo-Filho, Mario [Instituto Nacional do Cancer, Rio de Janeiro, RJ (Brazil)

    1996-07-01

    The labelling of red blood cells (RBC) with technetium-99m (Tc-99m) have been used for many studies in nuclear medicine. This labeling procedure depends on a reducing agent and stannous chloride is normally used for this purpose. Many factors, including drug therapy, can affect the biodistribution of radiopharmaceuticals, such factors many lead to poor organ visualization, requirement to repeat the examination procedure or even misdiagnosis. Guarana (Paullinia cupana) is commonly used in popular medicine. Here, we investigate if guarana is capable to alter the labeling of RBC with Tc-99m and to modify the radioactivity binding to plasma proteins and cellular constituents from insoluble and soluble fractions. The results were obtained with blood samples from Wistar rats with heparine and incubated with different guarana solutions. The percentage of radioactivity (% rad) from BC and P were calculated. The distribution of radioactivity in P and BC showed that the uptake of Tc-99m decreased significantly in all concentrations. (author)

  8. Preparation and In Vitro Evaluation of Tc-99m Labeled Octreotide%99mTc-octreotide的标记及受体分析

    Institute of Scientific and Technical Information of China (English)

    叶千春; 吴翼伟; 范我; 江一民; 章斌

    2003-01-01

    目的探讨直接法标记99mTc-octreotide的方法学及其与大鼠脑皮质细胞的亲和力.方法用直接法标记99mTc-octreotide,探讨其最佳标记条件;并以99mTc-octreotide作为配基,检测大鼠脑皮质细胞与其结合的平衡解离常数.结果99mTc-octreotide标记率为53%~57%,经Sephadex G-10纯化后放化纯大于95%;受体分析显示大鼠脑皮质细胞与99mTc-octreotide保持了较高的亲和力(Kd=10.4nM).结论本标记方法是可行的,99mTc-octreotide有希望作为具有临床实用价值的生长抑素受体显像剂.

  9. Characterization of technetium-99m-L,L-ECD for brain perfusion imaging, Part 1: Pharmacology of technetium-99m ECD in nonhuman primates

    Energy Technology Data Exchange (ETDEWEB)

    Walovitch, R.C.; Hill, T.C.; Garrity, S.T.; Cheesman, E.H.; Burgess, B.A.; O' Leary, D.H.; Watson, A.D.; Ganey, M.V.; Morgan, R.A.; Williams, S.J. (E.I. Du Pont de Nemours Co., Inc., No. Billerica, MA (USA))

    1989-11-01

    Technetium-99m ethyl cysteinate dimer (({sup 99m}Tc)ECD) is a neutral, lipophilic complex which rapidly crosses the blood-brain barrier. Brain retention and tissue metabolism of ({sup 99m}Tc)ECD is dependent upon the stereochemical configuration of the complex. While both L,L and D,D enantiomers are extracted by the brain, only the L,L but not the D,D form, is metabolized and retained in the monkey brain (4.7% injected dose initially, T 1/2 greater than 24 hr). Dynamic single photon emission computed tomography imaging studies in one monkey indicates {sup 99m}Tc-L,L-ECD to be distributed in a pattern consistent with regional cerebral blood flow for up to 16 hr postinjection. Dual-labeled {sup 99m}Tc-L,L-ECD and ({sup 14}C)iodoantipyrine autoradiography studies performed 1 hr after administration show cortical gray to white matter ratios of both isotopes to be equivalent (approximately 4-5:1). These data suggest that {sup 99m}Tc-L,L-ECD will be useful for the scintigraphic assessment of cerebral perfusion in humans.

  10. Synthesis, characterization, biodistribution and scintigraphy of 99mTc-paclitaxel. A potential tracer of paclitaxel

    International Nuclear Information System (INIS)

    99mTc-paclitaxel was synthesized by using sodium borohydride as a reducing agent. Greater than 95 % labelling efficiency was achieved. Radiochemical purity of the synthesized 99mTc-paclitaxel was validated by thin layer chromatography (TLC) scanner and high performance liquid chromatography (HPLC). 99mTc-paclitaxel passed in vitro stability tests. Biodistribution and scintigraphy studies were performed in Sprague-Dawley rats. The biodistribution study results of 99mTc-paclitaxel were related mainly to the metabolism and excretion routes followed by the parental drug, paclitaxel. Apart from that, biodistribution of 99mTc-paclitaxel was altered after pre-treatment with cold paclitaxel. Hence, 99mTc-paclitaxel may be used as a tracer for paclitaxel. (author)

  11. Organ distribution of sup(99m)Tc-Sn tetracycline antibiotics in rats

    International Nuclear Information System (INIS)

    The organ distribution of [sup(99m)Tc-Sn]tetracycline hydrochloride, [sup(99m)Tc-Sn]oxytetracycline hydrochloride and [sup(99m)Tc-Sn]rolitetracycline nitrate was studied in rats. It was shown that these preparations have a very similar organ distribution and are predominantly deposited in the kidneys. The maximum renal radioactivity level was found 2 to 4 hours after intravenous administration of the preparation, this, for [sup(99m)Tc-Sn]tetracycline hydrochloride 18.60%; for [sup(99m)Tc-Sn]oxytetracycline hydrochloride 17.09.=.; for [sup(99m)Tc-Sn]rolitetracycline nitrate 20.12%. The activity levels in the muscle, liver, heart, brain, lungs, stomach and spleen are minimal. (author)

  12. 99mTc-MAMA-HA与99mTcN-MAMA-HA的制备及生物性能的比较%THE PREPARATION AND BIOLOGICAL PROPERTIES OF 99mTc-MAMA-HA AND 99mTcN-MAMA-HA

    Institute of Scientific and Technical Information of China (English)

    吴战宏; 王学斌; 李至敏

    2002-01-01

    制备了2种脂肪酸标记物99mTc-MAMA-HA和99mTcN-MAMA-HA,改进了标记方法,放化纯均大于90%;对标记物进行了小鼠的生物分布实验,结果表明二者都有一定的心肌摄取,99mTc-MAMA-HA的心肌摄取要好于99mTcN-MAMA-HA,而后者的心肌滞留要好于前者,这说明99mTcN的引入明显改变了99mTc-MAMA-HA的生物分布.

  13. GAMMAGRAFÍA CON 99TC-MIBI PARA DETERMINAR LA EFECTIVIDAD DE LA HEBERQUINASA PARA REPERFUNDIR LA ARTERIA RELACIONADA CON EL INFARTO / 99tc-mibi gammagraphy to determine Heberkinase effectiveness in the reperfusion of the infarction-related artery

    OpenAIRE

    Marilyn Ramírez Méndez; Luis Castañeda Casarvilla; Iguer F. Aladro Miranda; Francisco Luis Moreno-Martínez; Carlos M. Osorio Gómez; Rudy Hernández Ortega; Marisel I. Noda Valledor

    2010-01-01

    Resumen Introducción y objetivos: La utilización de técnicas nucleares para la determinación de la permeabilidad de la arteria relacionada con el infarto, es de gran importancia clínica. El objetivo de esta investigación fue conocer el estado de la perfusión miocárdica del territorio dependiente de la irrigación de esa arteria y determinar la presencia o no de su reapertura después de la trombólisis. Método: Se realizó una investigación experimental puntual con una muestra de 10 pacientes con...

  14. Physicochemical and biological study of a renal scintigraphy agent: the DMSA - 99mTc complex

    International Nuclear Information System (INIS)

    This research thesis deals with the study of the dimercaptosuccinic acid (DMSA) marked with 99mTc, a recently developed scintigraphy agent used for the kidney isotopic exploration. The author notably studied the relationships between the physicochemical properties of solutions of dimercaptosuccinic acid marked with 99mTc and the biological distribution of 99mTc in order to reach a better understanding of the biological mechanism which results in technetium fixation to the kidney

  15. Diagnosis of orbital cavernous hemangioma with Tc-99m RBC SPECT.

    Science.gov (United States)

    Ki, W W; Shin, J W; Won, K S; Ryu, J S; Yang, S O; Lee, H K; Kim, Y J

    1997-08-01

    The authors report two cases of orbital cavernous hemangioma diagnosed by Tc-99m RBC SPECT. Tc-99m RBC SPECT showed a typical scintigraphic pattern commonly seen in hepatic hemangioma in which there is intense focally increased uptake on delayed SPECT images. Tc-99m RBC SPECT in orbital cavernous hemangioma may be as useful a diagnostic modality as in hepatic hemangioma. PMID:9262901

  16. Study with radio aerosol of DTPA technetium-99 m in individuals with pulmonary disease by amiodarone

    International Nuclear Information System (INIS)

    In order to evaluate the role of the clearance of 99 m Technetium chelated to diethylenetriamine-penta-acetate (99 m Tc-DTPA) in amiodarone induced pulmonary disease, 40 individuals were studied in four groups. After spirometry, where a volume-time curve was registered, all individuals inhaled 740 MBq of 99 m Tc-DTPA diluted in 4 ml of saline, for five minutes. Pulmonary images were obtained in a computerized scintillation camera and 9 regions of interest were selected. (author)

  17. Detection and Characterization of Parathyroid Adenoma/Hyperplasia for Preoperative Localization: Comparison Between {sup 11}C-Methionine PET/CT and {sup 99m}Tc-Sestamibi Scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Chun, In Kook; Cheon, Gi Jeong; Paeng, Jin Chul; Kang, Keon Wook; Chung, Junekey; Lee, Dong Soo [Seoul National Univ. College of Medicine, Seoul (Korea, Republic of)

    2013-09-15

    {sup 11}C-Methionine PET/CT (Met-PET/CT) is a useful imaging method for detection of parathyroid adenoma; however, the reported detection rate has been variable. The current study was intended to investigate detection sensitivity and preoperative localization of parathyroid adenoma (PA) or parathyroid hyperplasia (PH) on Met-PET/CT compared with {sup 99m}Tc-sestamibi (MIBI) scintigraphy in patients with primary hyperparathyroidism (HPT) or suspected PA. Met-PET/CT and MIBI scintigraphy images were reviewed by two nuclear medicine physicians unaware of pathologic results. Detection sensitivities and preoperative localization of detected parathyroid tissues into five predefined segments were evaluated by visual assessment and semiquantitative analysis with ratio of standardized uptake values (SUVR) between parathyroid tissue and normal lung as reference. Linear regression analysis with SUVR and serum parathyroid hormone (sPTH) was performed for characterization of PA or PH. Predicted PTH (pPTH) was calculated and compared with sPTH in PH and PA. Each pPTH was obtained for a calculated SUVR by using linear regression model from the result of previous linear regression analysis between SUVR and sPTH. In 16 patients, detection sensitivities of Met-PET/CT and MIBI scintigraphy were 91.7 % (11/12) and 41.7 % (5/12) for PA and PH including both biopsy-confirmed and clinically-suspected cases, and 100 % (8/8) and 50 % (4/8) for pathologically confirmed PA and PH cases only, respectively. Met-PET/CT showed higher performance than MIBI scintigraphy in localization of parathyroid tissues; correct localization rate was 87.5 % (7/8) on Met-PET/CT and 50 % (4/8) on MIBI scintigraphy. In semi-quantitative analysis, SUVR was linearly associated with sPTH by linear regression analysis (sPTH=39.53ΧSUVR-89.84, p=0.0383). There was a borderline significant difference in pPTH between PH and PA (35.1 vs 204.7±164.0, p=0.052),while there was no significant difference in sPTH between PH

  18. Technetium-99m labeling anti-amastigote polyclonal antibodies of Leishmania amazonensis

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, J.G.V.C.; Toledo, V.P.C.P.; Guimaraes, T.M.P.D.; Bernardo-Filho, M.; Simal, C.J.R.; Mota, L.G.; Diniz, S.O.F.; Cardoso, V.N. E-mail: cardosov@farmacia.ufmg.br

    2002-05-01

    Anti-amastigote polyclonal antibody (IgG) was incubated with solutions of stannous chloride and sodium borohidride. After that, 3.7 MBq of technetium-99m ({sup 99m}Tc) was added. A labeling yield of the antibody about 84% was obtained. After filtration of {sup 99m}Tc-IgG, the radiochemical purity increased from 84 to 95%. The labeling of IgG with {sup 99m}Tc did not modify the immunoreactivity of the antibody, since it was able to identify in vitro and in vivo the specific antigen of Leishmania amazonensis.

  19. A new technology of technetium-99m production for medicine imaging

    Energy Technology Data Exchange (ETDEWEB)

    Dovbnya, A.N.; Dikiy, N.P.; Medvedyeva, Ye.P.; Tur, Yu.D.; Uvarov, U.L. [National Science Center Kharkov Inst. of Physics and Technology, Kharkov (Ukraine)

    1998-07-01

    Technetium-99m provides now up to 90% of medical analysis using gamma-scintigraphy method. The parent isotope {sup 99} Mo is produced mainly in fission reactors, that is rather technically complicated and ecologically unwonted. The report contains a short description of new approach to {sup 99m} Tc production problem using irradiation of Mo-target with mixed e, n, {gamma}-radiation of electron accelerator and extraction of {sup 99m} Tc by electrolysis. Medical test of {sup 99m} Tc based radiopharmaceutical thus produced their high isotope purity. (author)

  20. Effective detection of the tumors causing osteomalacia using [Tc-99m]-HYNIC-octreotide (99mTc-HYNIC-TOC) whole body scan

    Energy Technology Data Exchange (ETDEWEB)

    Jing, Hongli, E-mail: annsmile1976@sina.com [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Li, Fang, E-mail: lifang@pumch.cn [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Zhuang, Hongming, E-mail: zhuang@email.chop.edu [Department of Radiology, The Children' s Hospital of Philadelphia, University of Pennsylvania Perelman School of Medicine, 34th and Civic Ctr Blvd, Philadelphia, PA 19104 (United States); Wang, Zhenghua, E-mail: ccq1214@yahoo.com.cn [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Tian, Jian, E-mail: tianjian4809@hotmail.com [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Xing, Xiaoping, E-mail: xingxp@126.com [Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Jin, Jin, E-mail: jinjin9010@126.com [Department of Orthopedic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Zhong, Dingrong, E-mail: ZhongDR@pumch.cn [Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Zhang, Jingjing, E-mail: zhangjingjingtag@163.com [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China)

    2013-11-01

    Purpose: Tumor-induced osteomalacia (TIO) is an endocrine disorder caused by tumors producing excessive fibroblast growth factor-23 (FGF-23). The causative tumors are generally small, slow-growing benign mesenchymal tumors. The only cure of the disease depends on resection of the tumors, which are extremely difficult to localize due to their small sizes and rare locations. Since these tumors are known to express somatostatin receptors, this research was undertaken to evaluate efficacy of [Tc-99m]-HYNIC-octreotide (99mTc-HYNIC-TOC) whole body imaging in this clinical setting Methods: Images of 99mTc-HYNIC-TOC scans and clinical chart from 183 patients with hypophosphatemia and clinically suspected TIO were retrospectively reviewed. The scan findings were compared to the results of histopathological examinations and clinical follow-ups. Results: Among 183 patients, 72 were confirmed to have TIO while 103 patients were found to have other causes of hypophosphatemia. The possibility of TIO could not be either diagnosed or excluded in the remaining 8 patients. For analytical purposes, these 8 patients who could neither be diagnosed nor excluded as having TIO were regarded as having the disease, bringing the total of TIO patients to 80. The 99mTc-HYNIC-TOC scan identified 69 tumors in 80 patients with TIO, which rendered a sensitivity of 86.3% (69/80). 99mTc-HYNIC-TOC scintigraphy excluded 102 patients without TIO with a specificity of 99.1% (102/103). The overall accuracy of 99mTc-HYNIC-TOC whole body scan in the localization of tumors responsible for osteomalacia is 93.4% (171/183). Conclusions: Whole body 99mTc-HYNIC-TOC imaging is effective in the localization of occult tumors causing TIO.

  1. Effective detection of the tumors causing osteomalacia using [Tc-99m]-HYNIC-octreotide (99mTc-HYNIC-TOC) whole body scan

    International Nuclear Information System (INIS)

    Purpose: Tumor-induced osteomalacia (TIO) is an endocrine disorder caused by tumors producing excessive fibroblast growth factor-23 (FGF-23). The causative tumors are generally small, slow-growing benign mesenchymal tumors. The only cure of the disease depends on resection of the tumors, which are extremely difficult to localize due to their small sizes and rare locations. Since these tumors are known to express somatostatin receptors, this research was undertaken to evaluate efficacy of [Tc-99m]-HYNIC-octreotide (99mTc-HYNIC-TOC) whole body imaging in this clinical setting Methods: Images of 99mTc-HYNIC-TOC scans and clinical chart from 183 patients with hypophosphatemia and clinically suspected TIO were retrospectively reviewed. The scan findings were compared to the results of histopathological examinations and clinical follow-ups. Results: Among 183 patients, 72 were confirmed to have TIO while 103 patients were found to have other causes of hypophosphatemia. The possibility of TIO could not be either diagnosed or excluded in the remaining 8 patients. For analytical purposes, these 8 patients who could neither be diagnosed nor excluded as having TIO were regarded as having the disease, bringing the total of TIO patients to 80. The 99mTc-HYNIC-TOC scan identified 69 tumors in 80 patients with TIO, which rendered a sensitivity of 86.3% (69/80). 99mTc-HYNIC-TOC scintigraphy excluded 102 patients without TIO with a specificity of 99.1% (102/103). The overall accuracy of 99mTc-HYNIC-TOC whole body scan in the localization of tumors responsible for osteomalacia is 93.4% (171/183). Conclusions: Whole body 99mTc-HYNIC-TOC imaging is effective in the localization of occult tumors causing TIO

  2. Development of Tc99m-Saccharic Acid for Heart Imaging

    International Nuclear Information System (INIS)

    Cardiovascular disease especially the coronary heart disease (CHD) is the leading cause of death worldwide. Coronary heart disease is a common term for the buildup of plaque in the heart coronary arteries that could block the blood supply to the myocardial and this could lead to heart attack. An estimated 17 million people died from cardiovascular disease in 2008 representing 30% of all global death. In United Kingdom, coronary heart disease killed as much as 82,000 people each year. Hence, early detection of the coronary heart disease is very important in reducing the mortality among the world population. One of the most sensitive detection methods is by radioimaging using Technetium-99m radiopharmaceuticals. Several different radio imaging agents such as Tc99m radiopharmaceutical were developed as radiagnostic agent in determining the CHD especially in identifying the blockage of the coronary artery of the heart muscle. Despite the success of Tc99m-sestamibi and Tc99m-tetrofosmin as effective agents for myocardial perfusion study, the search for other Tc99m heart imaging agents has never been interrupted. This report described the formulation of the Tc99m-saccharic acid radiopharmaceutical kit, radiolabelling of the kit, radiochemical purity evaluation of the Tc99m labeled saccharaic acid, and animal study involving radio imaging using gamma camera. The animal are then sacrificed and the biological distribution of the Tc99m-saccharic acid in-vivo was determined. Comparative study was also conducted using commercially available Tc99m-tetrafosmin, a CHD radiopharmaceutical kit. The Tc99m-saccharic acid developed gave a very high labeling efficiency of >92% with Tc99m and good uptake in the heart muscle. The saccharic acid kit developed was also found to be comparable in quality to the commercially available Tc99-tetrafosmin kit. (author)

  3. Determination of 99Mo contamination in a nuclear medicine patient submitted to a diagnostic procedure with 99mTc

    Directory of Open Access Journals (Sweden)

    Bernardo Maranhão Dantas

    2005-10-01

    Full Text Available 99mTc is a radionuclide widely used for imaging diagnosis in nuclear medicine. In Brazil it is obtained by elution from 99Mo-99mTc generators supplied by the Nuclear Energy Research Institute (IPEN. The elution is carried out in radiopharmacy laboratories located in hospitals and clinics. Depending of the quality of the generator and conditions of use during the elution process, 99Mo can be extracted from the column of the generator, becoming a radionuclidic impurity of the eluate used for the obtention of the radiopharmaceutical to be administered to the patient. 99Mo emits high-energy photons and beta particles and its presence degrades the quality of the image and unnecessarily increases the radiation dose delivered to the patient. An in-vivo measurement technique was developed to verify the occurrence of internal contamination by 99Mo in nuclear medicine patients. Direct measurements were made in a volunteer who underwent myocardial scintigraphy with 99mTc-sestamibi. The results indicated the presence of internal contamination of the patien by 99Mot. The activity was tracked for several days, and an assessment of the radiation dose from the contaminant 99Mo was made.O 99mTc é um radionuclídeo largamente utilizado em diagnósticos por imagem em medicina nuclear. No Brasil, ele é obtido por eluição de um gerador de 99Mo-99mTc fornecido pelo IPEN. A eluição do gerador é feita nas clínicas onde se realizam os exames. Durante a eluição o 99Mo pode ser carreado da coluna, tornando-se uma impureza radionuclídica do eluato a ser utilizado para a obtenção do radiofármaco administrado ao paciente. O 99Mo emite fótons de alta energia e partículas beta, e sua presença, além de provocar degradação na qualidade da imagem do exame, aumenta desnecessariamente a dose de radiação no paciente. Assim, com o objetivo de verificar a possível ocorrência de contaminação interna por 99Mo em pacientes de medicina nuclear, foi desenvolvida

  4. {sup 99m}Tc-interleukin-2 and {sup 99m}Tc-HMPAO granulocyte scintigraphy in patients with inactive Crohn's disease

    Energy Technology Data Exchange (ETDEWEB)

    Annovazzi, Alessio; Chianelli, Marco; Capriotti, Gabriela; Signore, Alberto [Nuclear Medicine Unit, Department of Clinical Sciences, Policlinico Umberto I, University ' ' La Sapienza' ' , 00161, Rome (Italy); Biancone, Livia; Pallone, Francesco [Gastroenterology Unit, University ' ' Tor Vergata' ' , Rome (Italy); Caviglia, Renato [Gastroenterology Unit, University ' ' Campus Biomedico' ' , Rome (Italy); Mather, Stephen J. [Department of Nuclear Medicine, St Bartholomew' s Hospital, Cancer Research UK, London (United Kingdom); Caprilli, Renzo [Gastroenterology Unit, Department of Clinical Sciences, University ' ' La Sapienza' ' , Rome (Italy); Scopinaro, Francesco [Department of Radiological Sciences, University ' ' La Sapienza' ' , Rome (Italy)

    2003-03-01

    Crohn's disease (CD) is a chronic inflammatory bowel disease that may involve the whole gut. Marked intestinal T cell and macrophage activation is a key feature of the disease. Polymorphonuclear cell infiltration is also observed in the diseased gut, mainly during active inflammation. Scintigraphic detection of granulocytes and activated lymphocytes infiltrating the gut wall may be useful in identifying a subgroup of patients with clinically inactive CD who are undergoing early clinical relapse. The aims of the present study were (a) to compare the effectiveness of scintigraphy with {sup 99m}Tc-labelled interleukin-2 ({sup 99m}Tc-IL2) and with {sup 99m}Tc-HMPAO labelled granulocytes ({sup 99m}Tc-WBC) in detecting the presence and extent of bowel inflammation in patients with long-term inactive CD (>12 months) and (b) to assess the accuracy of these techniques in predicting future disease relapse. We studied 29 patients with ileal and/or colonic CD in stable clinical remission (Crohn's Disease Activity Index <150 for at least 12 months) using both {sup 99m}Tc-IL2 and {sup 99m}Tc-WBC scintigraphy in order to evaluate the extent of acute and chronic inflammation in the bowel. Planar and single-photon emission tomography images were acquired in each patient at 1 h p.i. For quantitative analysis of {sup 99m}Tc-IL2 uptake, the abdomen was divided into 32 regions of interest. Despite the absence of symptoms, 18 patients (62%) showed a positive {sup 99m}Tc-IL2 and 18 (62%) a positive {sup 99m}Tc-WBC scan. Only 12 patients (41.4% of the total group) were positive on both scans, and the sites of IL2 and granulocyte bowel uptake were usually located in different segments, indicating that in CD, acute and chronic inflammation can be present in different sites. As far as the prognostic role of the two scans in predicting future disease relapse is concerned, both {sup 99m}Tc-IL2 and {sup 99m}Tc-WBC scintigraphy showed a high negative predictive value (1.00 and 0

  5. Genotoxic effect of radio marked lymphocytes using Tc-99m complexes; Efecto genotoxico del radiomarcado de linfocitos empleando complejos de Tc-99m

    Energy Technology Data Exchange (ETDEWEB)

    Pedraza L, M.; Ferro F, G.; Mendiola C, M.T.; Morales R, P. [Instituto nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1997-07-01

    The genotoxic effect of radio marked lymphocytes was evaluated using {sup 99m}-Tc-HMPAO and {sup 99m}-Tc- gentisic acid complexes. With the results of this work it is pretended to contribute to the knowledge of genetic and structural damages that provokes the radiation in the marked lymphocytes. The d, 1-HMPAO was synthesized in laboratory with a yielding of 30 %. The radiochemical purity of the complexes was greater than 85%. Mouse lymphocytes obtained of sanguineous volumes 2 ml were used. The radio marked efficiency of cells was 19.6 {+-} 6.4% and 25.6 {+-} 5.8% for {sup 99m}Tc-HMPAO and {sup 99m} Tc gentisic acid respectively. The genotoxic effect was evaluated using the technique of Unicellular Electrophoresis in Micro gel (Comet assay). The results showed that both {sup 99m} Tc complexes produce genotoxicity due to their capacity to penetrate cells, therefore the Auger and M internal conversion electrons place all their energy obtaining doses of Gray order. (Author)

  6. Diversification of 99Mo/99mTc separation: non–fission reactor production of 99Mo as a strategy for enhancing 99mTc availability.

    Science.gov (United States)

    Pillai, Maroor R A; Dash, Ashutosh; Knapp, Furn F Russ

    2015-01-01

    This paper discusses the benefits of obtaining (99m)Tc from non-fission reactor-produced low-specific-activity (99)Mo. This scenario is based on establishing a diversified chain of facilities for the distribution of (99m)Tc separated from reactor-produced (99)Mo by (n,γ) activation of natural or enriched Mo. Such facilities have expected lower investments than required for the proposed chain of cyclotrons for the production of (99m)Tc. Facilities can receive and process reactor-irradiated Mo targets then used for extraction of (99m)Tc over a period of 2 wk, with 3 extractions on the same day. Estimates suggest that a center receiving 1.85 TBq (50 Ci) of (99)Mo once every 4 d can provide 1.48-3.33 TBq (40-90 Ci) of (99m)Tc daily. This model can use research reactors operating in the United States to supply current (99)Mo needs by applying natural (nat)Mo targets. (99)Mo production capacity can be enhanced by using (98)Mo-enriched targets. The proposed model reduces the loss of (99)Mo by decay and avoids proliferation as well as waste management issues associated with fission-produced (99)Mo.

  7. Effect of passiflora quadrangularis extracts on the technetium-99m biodistribution in mice; Efeito de extratos do maracuja-acu na biodistribuicao do tecnecio-99m em camundongos

    Energy Technology Data Exchange (ETDEWEB)

    Bezerra, A.L.; Souza, G.M.L.; Nascimento, E.V.; Carvalho, E.B.; Catanho, M.T.J.A. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Biofisica] E-mail: mariajansem@hotmail.com

    2002-07-01

    Medicinal plants have been evaluated and their effect on the biodistribution of radiopharmaceuticals are very important. This work aims to evaluate the interaction of extracts of Passiflora quadrangularis on the biodistribution of {sup 99m}Tc in mice (Mus musculus). The animals were submitted to brief treatment with aqueous (G1) and hydroalcoholic 10% (G2) extracts. Control animals received saline solution 0.9% (n=5). The reaction {sup 99m}Tc (3.7 MBq) in the presence of stannous chloride and extracts was incubated for 10 minute, then it was injected (ip way) in mice. At the end of 30 minutes, animals were sacrificed for removal of organs and 0.5 mL of blood, which were counted in gamma counter. The percentage of {sup 99m}Tc-binding was calculated in relation to the weight of each organ. The results show a reduction on right and left kidneys in the presence of aqueous extract at about 26.74%, and more significant reduction in the presence of hydroalcoholic 10% extract at about 72.81%. the trachea presents a binding capacity to {sup 99m}Tc in order of 263.5% in the presence of aqueous extract. These results demonstrate that {sup 99m}Tc presents affinity to some organs and that the extract of Passiflora quadrangularis is able to alter this labeling. Therefore, more attention has been given to diagnoses with pharmaceutical compounds labeled with radioisotopes. (author)

  8. Hepatic clearance of {sup 99m}Tc-GSA in cases of postoperative biliary atresia. A retrospective comparison with hepatic clearance of {sup 99m}Tc-PMT

    Energy Technology Data Exchange (ETDEWEB)

    Yamazaki, Tetsuro; Yamada, Shogo [Tohoku Univ., Sendai (Japan). School of Medicine; Maruoka, Shin

    1998-11-01

    There are a few reports about usefulness of {sup 99m}Tc-GSA liver scintigraphy (GSA imaging) for the evaluation of postoperative state of biliary atresia. However, comparison of significance for the disease between GSA imaging and {sup 99m}Tc-PMT hepatobiliary imaging have not been reported. Fifty-six GSA imagings for 41 postoperative biliary atresia patients were reviewed and their scintigraphic findings and the functional parameters were compared with those obtained from {sup 99m}Tc-PMT hepatobiliary imaging. In quantitative analysis, although the hepatic clearance of both tracers calculated by graphical analysis (Patlak plot) were well correlated in most cases, a discrepancy was observed in several cases with jaundice. The authors conclude that the hepatic clearance of {sup 99m}Tc-GSA is a more accurate index of the hepatic reserve than that of {sup 99m}Tc-PMT which can be influenced by hyperbilirubinemia and that the combination of both examinations is helpful to evaluate development of liver injury in postoperative biliary atresia patients. (author)

  9. [Hepatic clearance of 99mTc-GSA in cases of postoperative biliary atresia--a retrospective comparison with hepatic clearance of 99mTc-PMT].

    Science.gov (United States)

    Yamazaki, T; Maruoka, S; Yamada, S

    1998-11-01

    There are a few reports about usefulness of 99mTc-GSA liver scintigraphy (GSA imaging) for the evaluation of postoperative state of biliary atresia. However, comparison of significance for the disease between GSA imaging and 99mTc-PMT hepatobiliary imaging have not been reported. Fifty-six GSA imagings for 41 postoperative biliary atresia patients were reviewed and their scintigraphic findings and the functional parameters were compared with those obtained from 99mTc-PMT hepatobiliary imaging. In quantitative analysis, although the hepatic clearance of both tracers calculated by graphical analysis (Patlak plot) were well correlated in most cases, a discrepancy was observed in several cases with jaundice. The authors conclude that the hepatic clearance of 99mTc-GSA is a more accurate index of the hepatic reserve than that of 99mTc-PMT which can be influenced by hyperbilirubinemia and that the combination of both examinations is helpful to evaluate development of liver injury in postoperative biliary atresia patients. PMID:9916401

  10. Diversification of 99Mo/99mTc separation: non–fission reactor production of 99Mo as a strategy for enhancing 99mTc availability.

    Science.gov (United States)

    Pillai, Maroor R A; Dash, Ashutosh; Knapp, Furn F Russ

    2015-01-01

    This paper discusses the benefits of obtaining (99m)Tc from non-fission reactor-produced low-specific-activity (99)Mo. This scenario is based on establishing a diversified chain of facilities for the distribution of (99m)Tc separated from reactor-produced (99)Mo by (n,γ) activation of natural or enriched Mo. Such facilities have expected lower investments than required for the proposed chain of cyclotrons for the production of (99m)Tc. Facilities can receive and process reactor-irradiated Mo targets then used for extraction of (99m)Tc over a period of 2 wk, with 3 extractions on the same day. Estimates suggest that a center receiving 1.85 TBq (50 Ci) of (99)Mo once every 4 d can provide 1.48-3.33 TBq (40-90 Ci) of (99m)Tc daily. This model can use research reactors operating in the United States to supply current (99)Mo needs by applying natural (nat)Mo targets. (99)Mo production capacity can be enhanced by using (98)Mo-enriched targets. The proposed model reduces the loss of (99)Mo by decay and avoids proliferation as well as waste management issues associated with fission-produced (99)Mo. PMID:25537991

  11. Study on technetium-99m labeling of graphene oxide nanosheets through click chemistry-99mTc labeling of graphene oxide nanosheets

    Institute of Scientific and Technical Information of China (English)

    江大卫; 彭程; 孙艳红; 贾丽娜; 李剑波; 张岚

    2015-01-01

    Graphene oxide (GO) nanosheets possess several advantages, such as a large surface, outstanding bio-compatibility, and straightforward chemical modification capability. They also have great potential as a drug-carrier. In this article, we radiolabeled GO nanosheets with 99mTc, which satisfies the potential needs of micro-SPECT imaging probes in pre-clinical and clinical research. GO nanosheets were synthesized through the modified Hummers’ method, then GO nanosheets with azide group covalently functionalized in two steps were conjugated to DOTA (1,4,7,10-tetraazacyclododecane-N,N0,N00,N000-tetraacetic acid) and functionalized with an alkynyl group by means of click chemistry. Then through the addition and reduction of technetium-99m, the 99mTc-DOTA-GO were attained. DOTA-conjugated GOs with lateral dimensions of 500–600 nm were synthe-sized. Both atomic force microscopy (AFM) and FT-IR were performed to characterize the GO-DOTA. Labeling efficiency of GO-DOTA with 99mTc was>90%and radiochemical purities were>96%with purification. We successfully synthesized graphene oxide derivatives, DOTA-conjugated GOs, via Click Chemistry, and it was labeled with 99mTc for SPECT imaging. High radiolabeling efficiency makes GO nanosheets suitable platforms for future molecular imaging research.

  12. Predictive value of brain SPECT with 99 technetium - MIBI for differentiation of histologic grade brain gliomas

    International Nuclear Information System (INIS)

    Diagnosis and treatment of primary tumors of the nervous system remain difficult and are a challenge to be addressed in a multidisciplinary way. In order to determine the usefulness of brain SPECT 99Tc MIBI to differentiate histologic grade brain gliomas - Frequently brain tumors - they were studied 68 patients with this technique. A dynamic study first step in AP and lateral view was performed, and a SPECT at 20 minutes post-administration and at 2 hours late views. the post-surgical histological study of injuries was used as control. several imaging parameters such as the absolute activity of 99mTc-MIBI were calculated both early and late phase, cortex contralateral tumor rates; pituitary tumor; choroid plexus tumor and Reason Late / Early phase tumor index / contralateral cortex tumor volume functional phase, the volume concentration of MIBI activity in the tumor and the retention rate of the radiopharmaceutical. Of the 68 patients studied, 11 were high-grade tumors and 57 low grade. The cortex contralateral tumor in late stage index showed a negative satisfactory sensitivity of 98.6% and specificity 77.1%, positive predictive value (PPV) of 48.2% and (NPV) of 99.8%. The reason late stage / early in the index tumor / contralateral cortex showed values ​​in turn 96.3%, 98.7%, 98.8% and 98.8% sensitivity, specificity, PPV and NPV respectively. The retention rate showed a 99% sensitivity, 89% specificity and PPV, NPV of 95% and 99% respectively. Conclusion: The combination cortex contralateral tumor rate in late stage, the reason late stage / early stage tumor index / contralateral cortex and the retention rate of the radiopharmaceutical are the most useful parameters to predict histologic grade of brain gliomas. (author)

  13. Scintigraphic evaluation of chronic osteomyelitis with technetium 99 m labeled polyclonal immunoglobulin

    International Nuclear Information System (INIS)

    Active chronic osteomyelitis or complicating osteomyelitis are difficult to be diagnosed by radiological imaging modalities, such as plain radiograph and CT. They frequently cause increased bone remodeling, leading to nonspecific uptake of Tc 99 m-bone scan agents and gallium-67. New radiopharmaceuticals with greater infection avidity are being developed, including the nonspecific polyclonal immunoglobulin (IgG) labeled with technetium 99 m. Tc 99 m-IgG may be available as a ready to use kit, with no reported side effects, low patient absorbed radiation dose and low cost. Material and Methods: 23 bone segments with suspected active chronic osteomyelitis or violated bone osteomyelitis were studied by Tc 99 m IgG scintigraphy. All patients underwent standard three phase bone scintigraphy using methylene diphosphonate (Tc 99 m MDP), gallium 67 scintigraphy and plain radiographs, compared with clinical evaluation and laboratory tests values. Results: Infection was found in 8 sites. Sensitivity and specificity for Tc-99 m MDP, gallium 67 and Tc 99 m IgG scintigraphy were, respectively, 88 and 36%, 75 and 73%, 88 and 82%. Conclusion: Tc-99 m IgG may be useful in the scintigraphic evaluation of osteomyelitis. (author)

  14. Imaging endocarditis with Tc-99m-labeled antibody--an experimental study: concise communication

    Energy Technology Data Exchange (ETDEWEB)

    Wong, D.W.; Dhawan, V.K.; Tanaka, T.; Mishkin, F.S.; Reese, I.C.; Thadepalli, H.

    1982-03-01

    The sensitivity and specificity of Tc-99m-labeled antibacterial antibody (Tc-99m Ab) for detecting bacterial endocarditis were evaluated in an experimental model. Rabbit-produced antistaphylococcal antibody was extracted using Rivanol and chemically labeled with Tc-99m. This Tc-99m Ab was injected intravenously in New Zealand rabbits 24 hr after producing Staphylococcus aureus endocarditis of the aortic valve. Imaging and tissue analyses were performed on the following day. All 11 animals developed S. aureus aortic-valve vegetations and showed increased uptake of Tc-99m Ab at the aortic valve, 118 times higher than at the uninfected tricuspid valve. Although high hepatic radioactivity and anatomic uncertainties interfered with in vivo delineation of these lesions, images of the excised hearts showed all affected valves. Two rabbits inoculated with Escherichia coli did not develop endocarditis and had little uptake of Tc-99m Ab, while six rabbits with enterococcal endocarditis had no uptake of the Tc-99m Ab in their vegetations. The findings suggest potential value of Tc-99m Ab on the rapid diagnosis of endocarditis.

  15. Establishment of experimental system for 99Mo/99mTc production by neutron activation method

    International Nuclear Information System (INIS)

    The research and development (R and D) on the production of 99Mo/99mTc by neutron activation method ((n, γ) method) using JMTR has been carried out in the Neutron Irradiation and Testing Reactor Center. The specific radioactivity of 99Mo by (n, γ) method is extremely low compared with that by fission method ((n,f) method), and as a result, the radioactive concentration of the obtained 99mTc solution is also low. Focusing on the solvent extraction method with methyl ethyl ketone (MEK) as the recovery method of 99mTc from 99Mo produced by (n, γ) method to solve the problem, we have developed the 99Mo/99mTc separation/extraction/concentration devices, and we have carried out the performance tests with the devices. In this report, in order to establish an experimental system for 99Mo/99mTc production, the R and D results of the system are summarized on the improvement of the devices for high-recovery rate of 99mTc, on the dissolution of the pellets, which is the high-density molybdenum trioxide (MoO3) pellets irradiated in Kyoto University Research Reactor (KUR), on the production of 99mTc, and on the inspection of the recovered 99mTc solutions. (author)

  16. Tc-99m-HMPAO brain scintigraphy. A comparative study with I-123-IMP

    Energy Technology Data Exchange (ETDEWEB)

    Nishimoto, Hitoshi; Yamamoto, Yoichi; Yoshida, Shoji; Akagi, Naoki; Ohara, Shuichi; Kamiike, Osamu; Maeda, Tomoho

    1989-04-01

    Sixteen single photon emission computed tomography (SPECT) studies with Tc-99m hexamethyl propylene amine oxime (Tc-99m HMPAO) were performed in patients with cerebral infarction (n=10), transient ischemic attack (n=2), or brain tumor (n=3). X-ray computed tomography (CT) scans and SPECT scans with N-isopropyl-p-(I-123)iodoamphetamine (I-123 IMP) were also obtained for comparison with findings of SPECT with Tc-99m HMPAO. Crossed cerebellar diaschisis (CCD) was detected in five patients (33%) on both early and delayed SPECT scans with Tc-99m HMPAO. In 8 patients, early scans with Tc-99m HMPAO tended to depict lesions more extensively when compared with CT. Early scanning with I-123 IMP was superior to that with Tc-99m HMPAO in detecting lesions. In detecting CCD, both methods were comparable to each other. In visualizing the basal ganglion, early scans with Tc-99m HMPAO were superior to scans with I-123 IMP. Tc-99m HMPAO has the advantages of short half-life period, small amounts of exposure doses, simple labeling, and prompt usage in emergency cases, such as cerebral infarction and cerebrovascular disorder. (Namekawa, K).

  17. In Vitro Evaluation of Tc-99m Radiopharmaceuticals for Gastric Emptying Studies

    Directory of Open Access Journals (Sweden)

    Türkan Ertay

    2014-02-01

    Full Text Available Objective: Gastrointestinal motility and functional motility disorders causing either delayed or accelerated gastric emptying (GE may result in similar symptoms including nausea, vomiting, early satiety, fullness, bloating, and abdominal discomfort or pain. Hence, it is important to evaluate patients for both rapid and delayed GE in the same test. The gold standard technique to measure GE is scintigraphy by radiolabeled test meals. The aim of this study was to test alternative Tc-99m agents to label eggs as the solid meal and compare to Tc-99m sulfur colloid (SC for gastric emptying studies. Methods: In search of alternative agents for gastric emptying studies, we mixed and fried eggs with four different particulate compounds (Tc-99m labeled SC, tin colloid, nanocolloid and MAA, as well as with free pertechnetate and Tc-99m DTPA. We then measured the stability of these compounds in simulated gastric juice. Results: Our experiments demonstrated that in addition to Tc-99m sulfur colloid;Tc-99m MAA, Tc-99m nanocolloid and Tc-99m tin colloid also appear to make stable complexes with eggs in acidic environment. Conclusion: Therefore, these agents may be used for gastric emptying studies which could be more practical in routine conditions.