WorldWideScience

Sample records for 99m clearance curves

  1. Effect of increased surface tension and assisted ventilation on /sup 99m/Tc-DTPA clearance

    Energy Technology Data Exchange (ETDEWEB)

    Jefferies, A.L.; Kawano, T.; Mori, S.; Burger, R.

    1988-02-01

    Experiments were performed to determine the effects of conventional mechanical ventilation (CMV) and high-frequency oscillation (HFO) on the clearance of technetium-99m-labeled diethylenetriamine pentaacetate (/sup 99m/Tc-DTPA) from lungs with altered surface tension properties. A submicronic aerosol of /sup 99m/Tc-DTPA was insufflated into the lungs of anesthetized, tracheotomized rabbits before and 1 h after the administration of the aerosolized detergent dioctyl sodium sulfosuccinate (OT). Rabbits were ventilated by one of four methods: 1) spontaneous breathing; 2) CMV at 12 cmH2O mean airway pressure (MAP); 3) HFO at 12 cmH2O MAP; 4) HFO at 16 cmH2O MAP. Administration of OT resulted in decreased arterial PO2 (PaO2), increased lung wet-to-dry weight ratios, and abnormal lung pressure-volume relationships, compatible with increased surface tension. /sup 99m/Tc-DTPA clearance was accelerated after OT in all groups. The post-OT rate of clearance (k) was significantly faster (P less than 0.05) in the CMV at 12 cmH2O MAP (k = 7.57 +/- 0.71%/min (SE)) and HFO at 16 cmH2O MAP (k = 6.92 +/- 0.61%/min) groups than in the spontaneously breathing (k = 4.32 +/- 0.55%/min) and HFO at 12 cmH2O MAP (4.68 +/- 0.63%/min) groups. The clearance curves were biexponential in the former two groups. We conclude that pulmonary clearance of /sup 99m/Tc-DTPA is accelerated in high surface tension pulmonary edema, and this effect is enhanced by both conventional ventilation and HFO at high mean airway pressure.

  2. Clearance of inhaled [sup 99m]Tc-DTPA predicts the clinical course of fibrosing alveolitis

    Energy Technology Data Exchange (ETDEWEB)

    Wells, A.U.; Hansell, D.M.; Harrison, N.K.; Bois, R.M. du (Interstitial Lung Disease Unit and Dept. of Radiology, Royal Brompton National Heart and Lung Hospital, London (United Kingdom)); Lawrence, R. (Royal Marsden Hospital, London (United Kingdom)); Black, C.M. (Royal Free Hospital, London (United Kingdom))

    1993-01-01

    The aim of this study was to determine whether the speed of technetium-labelled diethylene-triamine-pentacetate ([sup 99m]Tc-DTPA) clearance from the lung is predictive of disease progression in fibrosing alveolitis, as judged by changes in respiratory function tests. 82 nonsmoking patients with fibrosing alveolitis were studied (progressive systemic sclerosis, n=53, lone cryptogenic fibrosing alveolitis, n=29). Normal [sup 99m]Tc-DTPA clearance identified patients at risk of deterioration. Repeat measurement of clearance, approximately 12 months later, enabled the definition of a subgroup at higher risk, with persistently abnormal [sup 99m]Tc-DTPA clearance, and a smaller subgroup in whom reversion of clearance to normal was associated with a sustained improvement in respiratory function indices. These findings were not attributable to differences in treatment between subgroups. We conclude that the speed of [sup 99m]Tc-DTPA clearance discriminates between stable and progressive disease in fibrosing alveolitis. (au) (26 refs.).

  3. Does Technetium-99m Diethylenetriaminepentaacetate Clearance Predict the Clinical Course of Idiopathic Pulmonary Fibrosis?

    Directory of Open Access Journals (Sweden)

    Marco Mura

    2004-01-01

    Full Text Available Clearance of inhaled technetium-99m diethylenetriaminepentaacetate (99mTc-DTPA is a potential indicator of disease activity and progression in idiopathic pulmonary fibrosis (IPF. The objective of the present study was to evaluate the prognostic value of 99mTc-DTPA scans in IPF. A total of 22 patients (18 males, aged 33 to 80 years with IPF were followed for six to 20 months (mean 13 months. At diagnosis, high resolution computed tomography (HRCT scans showed a honeycomb pattern with bibasilar reticular opacities in all cases. At T0 (diagnosis and T1 (follow-up, each patient had pulmonary function tests (forced vital capacity, diffusing capacity of the lung for carbon monoxide and partial arterial O2 pressure, extension of fibrosis evaluated by HRCT visual score and 99mTc-DTPA lung clearance. Results at T0 and T1 were compared, taking into account the whole population and patients with relatively fast and slow 99mTc-DTPA wash-out. 99mTc-DTPA clearance did not show any significant correlation with functional tests or HRCT score. These findings indicate that clearance of inhaled 99mTc-DTPA is not of value in following the progress of IPF.

  4. [Quantification of separate renal function using Tc 99m DTPA and Tc 99m DMSA. Correlations between individual isotopic data and creatinine clearance].

    Science.gov (United States)

    Chevet, D; Moisan, A; Le Pogamp, P; Le Cloirec, J; Wehbe, B; Herry, J Y

    1984-01-01

    The purpose of this prospective study (30 controls and 100 renal patients) is to compare one to each other the values of renal function measured with: 1--the DTPA 99mTc , 2--the DMSA 99mTc , and 3--the creatinine clearance. After the control values being established, correlations are done for the whole group (n = 130) between the creatinine clearance, the DTPA 99mTc clearance and the fixation rate of the DMSA 99mTc . The correlations obtained are:--Clcr Versus Cldtpa = 0,91.--Clcr Versus % Dmsa = 0,90. - Cldtpa Versus % Dmsa = 0,93. and indicate the two isotopic tests performed give similar quantitative results than creatinine clearance. Camera and computer allow to measure the separate renal function one by one, the two isotopic test giving similar values for each kidney. The results of these studies give a good concordance with the observed diseases, except for partial renal artery stenosis. The pyelic retention of DMSA 99mTc product, in condition of ureteral compression, gives a potential overestimation of this test and is a matter to debate. The extreme simplicity and facility of DMSA 99mTc procedures make this investigation accessible to any patient and give excellent morphological and functional tests. DTPA 99mTc clearances--global and separate kidney--give a more sensitive approach than DMSA 99mTc does because vascular captation , elimination rates could be focused. DTPA 99mTc procedure is to be used in patients with transplanted kidney, silent IVP kidney, contrast media contraindication or for exploring glomerular clearance without urine collection.

  5. Residual kidney function after donor nephrectomy. Assessment by {sup 99m}Tc-MAG3-Clearance

    Energy Technology Data Exchange (ETDEWEB)

    Hamscho, N.; Doebert, N.; Menzel, C.; Berner, U.; Zaplatnikov, K.; Gruenwald, F. [Hospital of the J.W.G.-University, Frankfurt am Main (Germany). Dept. of Nuclear Medicine; Wilhelm, A.; Gossmann, J.; Scheuermann, E.H. [Hospital of the J.W.G.-University, Frankfurt am Main (Germany). Dept. of Internal Medicine

    2005-07-01

    Aim: We evaluated the long-term residual renal function after donor nephrectomy using {sup 99m}Tc-mercaptoacetyltriglycin (MAG3)-clearance. Donors, methods: Altogether 49 kidney donors were examined using {sup 99m}Tc-MAG3-clearance after nephrectomy for donation to a relative (m:f=11.38; age 55{+-}27 years). The donors were examined 16{+-}8 years postoperatively (1.5-26 years). 42 donors (86%) showed normal creatinine values, whereas the other seven (14%) exhibited slightly elevated levels. 20 donors were examined pre- and postoperatively and compared intraindividually. The kidney function was compared to the age adapted normal values of healthy persons with two kidneys (67-133% of age related mean). Results: After nephrectomy all donors showed a normal perfusion, good secretion, merely physiological intrarenal transit and a normal elimination from the kidneys. The {sup 99m}Tc-MAG3-clearance was 69{+-}15% of the normal mean value of healthy carriers of two kidneys regardless of the gender. 20 donors with a preoperative examination showed a significantly reduced total renal function from 84{+-}15% of the mean normal value preoperatively to 60{+-}15% postoperatively (p<0.005). 15 donors of this group exhibited a significant functional increase of the residual kidney from 40% initially to 60% after nephrectomy (p=0.003). No correlation was found between the initial {sup 99m}Tc-MAG3-clearance measured prior to nephrectomy and the clearance levels after nephrectomy. Also, no correlation between the preoperative {sup 99m}Tc-MAG3-clearance and the postoperative serum creatinine values could be observed. Althogether, 22% of the donors (11/49) developed arterial hypertension 10{+-}8 years after donation (1-23 years). This corresponds to the normal age prevalence of hypertension in the carriers of two kidneys. Three donors suffered from arterial hypertension prior to the operation. Conclusion: Kidney donors with normal or slightly elevated creatinine values postoperatively

  6. Effect of raised thoracic pressure and volume on 99mTc-DTPA clearance in humans

    Energy Technology Data Exchange (ETDEWEB)

    Nolop, K.B.; Maxwell, D.L.; Royston, D.; Hughes, J.M.

    1986-05-01

    Although positive airway pressure is often used to treat acute pulmonary edema, the effects on epithelial solute flux are not well known. We measured independently the effect of 1) positive pressure and 2) voluntary hyperinflation on the clearance of inhaled technetium-99m-labeled diethylenetriaminepentaacetic acid (/sup 99m/Tc-DTPA) in six nonsmokers and six smokers. Lung volumes were monitored by inductance plethysmography. Each subject was studied in four situations: 1) low end-expiratory volume (LO-), 2) low volume plus 9 cmH2O continuous positive airway pressure (LO+), 3) high end-expiratory volume (HI-), and 4) high volume plus continuous positive airway pressure (HI+). The clearance half time of 99mTc-DTPA for the nonsmokers decreased from 64.8 +/- 7.0 min (mean +/- SE) at LO- to 23.2 +/- 5.3 min at HI- (P less than 0.05). Positive pressure had no synergistic effect. The mean clearance half time for the smokers was faster than nonsmokers at base line but unaffected by similar changes in thoracic volume and pressure. We conclude that, in nonsmokers, positive airway pressure increases /sup 99m/Tc-DTPA clearance primarily through an increase in lung volume and that smokers are immune to these effects.

  7. Lung clearance of 99mTc-DTPA in systemic lupus erythematosus

    Directory of Open Access Journals (Sweden)

    P.T.R. Dalcin

    2002-06-01

    Full Text Available The early demonstration of lung involvement in systemic lupus erythematosus (SLE patients is a difficult but important task. In the present study we attempted to identify abnormalities in pulmonary clearance of 99mTc-DTPA in SLE, correlating their clearance data with clinical findings and disease activity. Forty-six consecutive SLE patients with and without active disease (LACC score and 30 normal volunteers were studied. All subjects were submitted to pulmonary scintigraphy with 99mTc-DTPA to evaluate the pulmonary clearance, and to a chest X-ray, and SLE patients were submitted to tests of disease activity, spirometry, arterial blood gases and tests to assess acute-phase proteins. Pulmonary clearance was faster in SLE patients with active disease when compared to normal controls [half-life of 67.04 min (51.52-82.55 min in active SLE versus 85.87 min (78.85-92.87 min in controls, P<0.05] and there was a higher frequency of abnormal clearance rates in patients with active disease (11 of 26 patients, 42.3% when compared with SLE patients without disease activity (2 of 20 patients, 10% (P = 0.04. A significant correlation was observed between the clearance rates and cough (P<0.05, but not between the clearance rates and dyspnea symptoms or radiological findings, duration of SLE disease, antinuclear antibody titers and patterns, C-reactive protein or anti-double stranded DNA antibodies. We conclude that the pulmonary clearance of 99mTc-DTPA is increased in SLE patients with active disease.

  8. Lung clearance of {sup 99m} Tc- DTPA in systemic lupus erythematous

    Energy Technology Data Exchange (ETDEWEB)

    Dalcin, P.T.R; Barreto, S.S.M.; Xavier, R.M.; Brenol, J.C.T. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Hospital de Clinicas. Dept. de Medicina Interna; Cunha, R.D.; Marroni, B.J. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Hospital de Clinicas. Servico de Medicina Nuclear]. E-mail: rxavier@hcpa.ufrgs.br

    2002-06-01

    The early demonstration of lung involvement in systemic lupus erythematosus (SLE) patients is a difficult but important task. In the present study we attempted to identify abnormalities in pulmonary clearance of 99 mTc-DTPA in SLE, correlating their clearance data with clinical findings and disease activity. Forty-six consecutive SLE patients with and without active disease (LACC score) and 30 normal volunteers were studied. All subjects were submitted to pulmonary scintigraphy with 99 mTc-DTPA to evaluate the pulmonary clearance, and to a chest X-ray, and SLE patients were submitted to tests of disease activity, spirometry, arterial blood gases and tests to assess acute-phase proteins. Pulmonary clearance was faster in SLE patients with active disease when compared to normal controls [half-life of 67.04 min (51.52-82.55 min) in active SLE versus 85.87 min (78.85-92.87 min) in controls, P<0.05] and there was a higher frequency of abnormal clearance rates in patients with active disease (11 of 26 patients, 42.3%) when compared with SLE patients without disease activity (2 of 20 patients, 10%) (P = 0.04). A significant correlation was observed between the clearance rates and cough (P<0.05), but not between the clearance rates and dyspnoea symptoms or radiological findings, duration of SLE disease, antinuclear antibody titers and patterns, C-reactive protein or anti-double stranded DNA antibodies. We conclude that the pulmonary clearance of 99 m Tc-DTPA is increased in SLE patients with active disease. (author)

  9. Technetium-99m mercaptoacetyltriglycine clearance: reference values for infants and children

    Energy Technology Data Exchange (ETDEWEB)

    Schofer, O. [Department of Pediatrics, Johannes-Gutenberg University, Mainz (Germany); Koenig, G. [Department of Pediatrics, Johannes-Gutenberg University, Mainz (Germany); Bartels, U. [Department of Pediatrics, Johannes-Gutenberg University, Mainz (Germany); Bockisch, A. [Department of Nuclear Medicine, Johannes-Gutenberg University, Mainz (Germany); Piepenburg, R. [Department of Nuclear Medicine, Johannes-Gutenberg University, Mainz (Germany); Beetz, R. [Department of Pediatrics, Johannes-Gutenberg University, Mainz (Germany); Meyer, G. [Department of Nuclear Medicine, Ludwig Maximilians University, Munich (Germany); Hahn, K. [Department of Nuclear Medicine, Ludwig Maximilians University, Munich (Germany)

    1995-11-01

    Six hundred and thirty-nine clearance studies performed in children aged 7 days to 19 years utilizing technetium-99m mercaptoacetyltriglycine (MAG 3) were retrospectively analysed. Standardized conditions for the investigation included: parenteral hydration (60 ml/hxm{sup 2} body surface) in addition to normal oral fluid intake, weight-related dose of {sup 99m}Tc-MAG 3 (1 MBq/kg body weight, minimum 15 MBq) and calculation of clearance according to Bubeck et al. Of the 513 children, 169 included in this analysis could be classified as ``normal`` with regard to their renal function. Normal kidney function was judged by the following criteria: normal GFR for age, normal tubular function (absence of proteinuria and glucosuria), normal renal parenchyma (on ultrasonography, MAG 3 scan and intravenous pyelography), absence of significant obstruction and gross reflux (> grade I) no single kidney and no difference in split renal function > 20%. Results showed increasing MAG 3 clearance values for infants during the first months of life, reaching the normal range for older children and adults between 7 and 12 months. (orig.)

  10. Effect of different levels of continuous positive airway pressure on the 99mTc-DTPA lung clearance

    Directory of Open Access Journals (Sweden)

    Dulciane Nunes Paiva

    2014-04-01

    Full Text Available Backgound and Objectives: Positive airway pressure continues (CPAP produces significant hemodynamic changes that may influence the variability of breathing pattern and heart rate, acting as an additional therapy to prevent atelectasis and to combat hypoxia. The rate of inhaled 99 m Technetiumdiethylenetriaminepentaacetic acid (99mTc-DTPA, along with changes in the lung epithelium cause an increase in the rate of clearance of this compound. The aim of this study was evaluate the pulmonary clearance rate of 99mTechnetium Diethylenetriaminepentaacetic acid (99mTc-DTPA through the use of different levels of CPAP. Methods: It was a quasi-experimental study involving 17 healthy individuals with normal lung functional. 99mTc-DTPA, as aerosol, was nebulized for 3 minutes with the individual in a sitting position. The pulmonary clearance rate was assessed through pulmonary scintigraphy under spontaneous breathing and under 20 and 10 cmH2 O CPAP in the sitting position. The clearance rate was expressed as the half-time (T½ that is the time for the activity to decrease to 50% of the peak value. Results: 20 cmH2 O CPAP produced significant reduction of the T½ of 99mTc-DTPA in the sitting position (p=0.005. However, 10 cmH2 O CPAP did not alter the T½ of DTPA in the same positions. Conclusion: High levels of continuos positive pressure in normal lungs resulted in faster 99mTc-DTPA clearance moreover, 10 cmH2 O did not alter its clearance rate. KEYWORDS: Noninvasive ventilation. Technetium Tc 99m Pentetate. Radionuclide Imaging.

  11. TECHNETIUM-99M-MAG3 CLEARANCE AS A PARAMETER OF EFFECTIVE RENAL PLASMA-FLOW IN PATIENTS WITH PROTEINURIA AND LOWERED SERUM-ALBUMIN LEVELS

    NARCIS (Netherlands)

    KENGEN, RA; MEIJER, S; BEEKHUIS, H; PIERS, DA

    1991-01-01

    Although the renal clearance of Tc-99m-MAG3 is about 60% of the I-131-hippurate clearance, Tc-99m-MAG3 clearance may be useful to estimate ERPF. In one study, however, proteinuria seemed to influence the MAG3/hippurate clearance ratio. In order to establish whether proteinuria or serum albumin level

  12. Stability, protein binding and clearance studies of [99mTc]DTPA. Evaluation of a commercially available dry-kit

    DEFF Research Database (Denmark)

    Rehling, M

    1988-01-01

    [99mTc]DTPA has achieved widespread use for the measurement of glomerular filtration rate (GFR) with the single injection plasma clearance technique and for gamma-camera renography. However, the quality of the commercial preparations varies. The purpose of the present investigation was to study...... the quality of a commercial [99mTc]DTPA preparation (C.I.S., France) with reference to stability, protein binding and accuracy of the determined plasma clearance values as a measure of GFR. The stability of the preparations was studied by thin-layer chromatography, the in vitro protein binding by Sephadex...... filtration after incubation with human serum albumin and in vivo protein binding by filtration of human plasma. The accuracy of the plasma clearance values was investigated by comparison with the simultaneously measured plasma clearance of [51Cr]EDTA. There was no detectable free pertechnetate or hydrolysed...

  13. Respiratory clearance of 99mTc-DTPA and pulmonary involvement in sarcoidosis

    Energy Technology Data Exchange (ETDEWEB)

    Dusser, D.J.; Collignon, M.A.; Stanislas-Leguern, G.; Barritault, L.G.; Chretien, J.; Huchon, G.J.

    1986-09-01

    To investigate the relationships between the respiratory epithelial clearance of micronic aerosolized /sup 99m/Tc-DTPA (RC-DTPA) and pulmonary function, serum angiotensin-converting enzyme (SACE), and lymphocytic alveolitis in patients with sarcoidosis, RC-DTPA was measured in 49 nonsmokers with pulmonary sarcoidosis and 38 normal nonsmokers. Pulmonary involvement was evaluated on chest roentgenograms (type O = normal, type I = hilar adenopathies, type II = hilar adenopathies associated with parenchymal shadows, type III = parenchymal shadows without adenopathy) and by pulmonary function tests. Serum angiotensin-converting enzyme was determined, and a bronchoalveolar lavage was performed for alveolar lymphocyte differential counting (Ly%). RC-DTPA was increased (greater than or equal to 1.96%/min) in 12 of 31 patients with type II or III involvement but was normal in all 18 patients with type O or I involvement (p = 0.002). Patients with increased RC-DTPA had low FVC, TLC, FEV1, and resting Pao2 (p less than 0.05); resting and exercise AaPo2 were increased (p less than 0.05), but RC-DTPA correlated negatively with FEV1 (p less than 0.01), Pao2 at rest (p less than 0.005), and DLCO (p less than 0.05) and positively with resting and exercise AaPO2 (p less than 0.01). In patients with increased RC-DTPA (42 +/- 17%), Ly% did not differ from Ly% in patients with normal RC-DTPA (34 +/- 16%). SACE was increased in patients with increased RC-DTPA (56 +/- 26 U/ml versus 38 +/- 16 U/ml; p = 0.007) and correlated positively with RC-DTPA (p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

  14. Stability, protein binding and clearance studies of [99mTc]DTPA. Evaluation of a commercially available dry-kit

    DEFF Research Database (Denmark)

    Rehling, M

    1988-01-01

    the quality of a commercial [99mTc]DTPA preparation (C.I.S., France) with reference to stability, protein binding and accuracy of the determined plasma clearance values as a measure of GFR. The stability of the preparations was studied by thin-layer chromatography, the in vitro protein binding by Sephadex...

  15. Clearance of technetium-99m-DTPA and HRCT findings in the evaluation of patients with Idiopathic Pulmonary Fibrosis

    Directory of Open Access Journals (Sweden)

    Karkavitsas Nikolaos

    2006-02-01

    Full Text Available Abstract Background Clearance of inhaled technetium-labeled diethylenetriamine pentaacetate (99mTc-DTPA is a marker of epithelial damage and an index of lung epithelial permeability. The aim of this study was to investigate the role of 99mTc-DTPA scan in patients with Idiopathic Pulmonary Fibrosis (IPF. Our hypothesis is that the rate of pulmonary 99mTc-DTPA clearance could be associated with extent of High Resolution Computed Tomography (HRCT abnormalities, cell differential of bronchoalveolar lavage fluid (BALF and pulmonary function tests (PFTs in patients with IPF. Methods We studied prospectively 18 patients (14 male, 4 female of median age 67yr (range 55–81 with histologically proven IPF. HRCT scoring included the mean values of extent of disease. Mean values of these percentages represented the Total Interstitial Disease Score (TID. DTPA clearance was analyzed according to a dynamic study using a Venticis II radioaerosol delivery system. Results The mean (SD TID score was 36 ± 12%, 3 patients had mild, 11 moderate and 4 severe TID. Abnormal DTPA clearance half-time (t1/2 Conclusion Our data suggest that 99mTc-DTPA lung scan is not well associated with HRCT abnormalities, PFTs, and BALF cellularity in patients with IPF. Further studies in large scale of patients are needed to define the role of this technique in pulmonary fibrosis.

  16. Stability, protein binding and clearance studies of [99mTc]DTPA. Evaluation of a commercially available dry-kit

    DEFF Research Database (Denmark)

    Rehling, M

    1988-01-01

    [99mTc]DTPA has achieved widespread use for the measurement of glomerular filtration rate (GFR) with the single injection plasma clearance technique and for gamma-camera renography. However, the quality of the commercial preparations varies. The purpose of the present investigation was to study...... the quality of a commercial [99mTc]DTPA preparation (C.I.S., France) with reference to stability, protein binding and accuracy of the determined plasma clearance values as a measure of GFR. The stability of the preparations was studied by thin-layer chromatography, the in vitro protein binding by Sephadex.......7% and 1.1%, respectively. The plasma clearance of [99mTc]DTPA was on an average 3.7% higher than that of [51Cr]EDTA in 27 patients. It is concluded that the [99mTc]DTPA preparation is reliable for the measurement of GFR. The preparation is stable for at least six hours at room temperature...

  17. Diagnostic accuracy of quantitative 99mTc-MIBI scintimammography according to ROC curve analysis

    Energy Technology Data Exchange (ETDEWEB)

    Kim, J. H.; Lee, H. K.; Seo, J. W.; Cho, N. S.; Cha, K. H.; Lee, T. H. [Gachon Medical College, Gil Medical Center, Inchon (Korea, Republic of)

    1998-07-01

    99mTc-sestamibi scintimammography (SMM) has been shown to be a useful diagnostic test in the detection of breast cancer and the receiver operating characteristic (ROC) curve analysis provides detailed information of a diagnostic test. The purpose of this study was to evaluate the feasibility and efficacy of quantitative indices of SMM in the detection of malignant breast lesions according to ROC analysis. Prone anterior, lateral planar and supine SPECT imagings were performed on 75 female patients (mean age=43.4 yr) with breast mass (size{>=}0.8cm) after intravenous injection of 20-30 mCi 99mTc-sestamibi. 45 Malignant (Invasive ductal ca(36), Inv lobular ca(5), Inv duc + lob (1), Inv tubular ca (3)) and 30 benign (fibroadenoma (13), fib cyst(12), Fat necrosis(3), papilloma(1), paraffinoma (1)) lesions were histologically proven. Data were analyzed by creating three regions of interest (ROIs) over designated areas: lesion, normal breast and right chest wall. Lesion to normal (L/NL) and lesion to chest wall (L/CW) ratios were calculated for each patient both on the planar and SPECT. The area under the ROC curve (AUC) was calculated and compared among four semiquantitative indices and an average scintimammographic index (SMM(mean)) from arithmatic mean. ROC curve analysis revealed planar L/N, SPECT L/N and L/CW ratios provide comparable better diagnostic accuracies for detection of breast cancer than planar L/CW ratio (p<0.05), respectively. For quantitative SMM of 75 lesions, malignancy rate was 60%, and Sensitivity, Specificity, Positive Predictive Value, Negative Predictive Value and Accuracy were 0.78, 0.77, 0.84, 0.72 and 0.77, respectively. Quantitative SMM is an useful objective method for differentiating malignant from benign breast lesions.

  18. Effects of positive expiratory pressure on pulmonary clearance of aerosolized technetium-99m-labeled diethylenetriaminepentaacetic acid in healthy individuals

    Science.gov (United States)

    de Albuquerque, Isabella Martins; Cardoso, Dannuey Machado; Masiero, Paulo Ricardo; Paiva, Dulciane Nunes; Resqueti, Vanessa Regiane; Fregonezi, Guilherme Augusto de Freitas; Menna-Barreto, Sérgio Saldanha

    2016-01-01

    ABSTRACT Objective: To evaluate the effects of positive expiratory pressure (PEP) on pulmonary epithelial membrane permeability in healthy subjects. Methods: We evaluated a cohort of 30 healthy subjects (15 males and 15 females) with a mean age of 28.3 ± 5.4 years, a mean FEV1/FVC ratio of 0.89 ± 0.14, and a mean FEV1 of 98.5 ± 13.1% of predicted. Subjects underwent technetium-99m-labeled diethylenetriaminepentaacetic acid (99mTc-DTPA) radioaerosol inhalation lung scintigraphy in two stages: during spontaneous breathing; and while breathing through a PEP mask at one of three PEP levels-10 cmH2O (n = 10), 15 cmH2O (n = 10), and 20 cmH2O (n = 10). The 99mTc-DTPA was nebulized for 3 min, and its clearance was recorded by scintigraphy over a 30-min period during spontaneous breathing and over a 30-min period during breathing through a PEP mask. Results: The pulmonary clearance of 99mTc-DTPA was significantly shorter when PEP was applied-at 10 cmH2O (p = 0.044), 15 cmH2O (p = 0.044), and 20 cmH2O (p = 0.004)-in comparison with that observed during spontaneous breathing. Conclusions: Our findings indicate that PEP, at the levels tested, is able to induce an increase in pulmonary epithelial membrane permeability and lung volume in healthy subjects. PMID:28117469

  19. Effects of positive expiratory pressure on pulmonary clearance of aerosolized technetium-99m-labeled diethylenetriaminepentaacetic acid in healthy individuals

    Directory of Open Access Journals (Sweden)

    Isabella Martins de Albuquerque

    Full Text Available ABSTRACT Objective: To evaluate the effects of positive expiratory pressure (PEP on pulmonary epithelial membrane permeability in healthy subjects. Methods: We evaluated a cohort of 30 healthy subjects (15 males and 15 females with a mean age of 28.3 ± 5.4 years, a mean FEV1/FVC ratio of 0.89 ± 0.14, and a mean FEV1 of 98.5 ± 13.1% of predicted. Subjects underwent technetium-99m-labeled diethylenetriaminepentaacetic acid (99mTc-DTPA radioaerosol inhalation lung scintigraphy in two stages: during spontaneous breathing; and while breathing through a PEP mask at one of three PEP levels-10 cmH2O (n = 10, 15 cmH2O (n = 10, and 20 cmH2O (n = 10. The 99mTc-DTPA was nebulized for 3 min, and its clearance was recorded by scintigraphy over a 30-min period during spontaneous breathing and over a 30-min period during breathing through a PEP mask. Results: The pulmonary clearance of 99mTc-DTPA was significantly shorter when PEP was applied-at 10 cmH2O (p = 0.044, 15 cmH2O (p = 0.044, and 20 cmH2O (p = 0.004-in comparison with that observed during spontaneous breathing. Conclusions: Our findings indicate that PEP, at the levels tested, is able to induce an increase in pulmonary epithelial membrane permeability and lung volume in healthy subjects.

  20. Lung clearance of /sup 99m/Tc-DTPA in patients with acute lung injury and pulmonary edema

    Energy Technology Data Exchange (ETDEWEB)

    Coates, G.; O' Brodovich, H.; Dolovich, M.

    1988-07-01

    Several acute and chronic conditions that alter the integrity of the pulmonary epithelium increased the rate of absorption or clearance into the circulation of small solutes deposited in the alveoli. Technetium 99m diethylenetriamine pentaacetic acid can be deposited in the lungs as a submicronic aerosol and its rate of clearance measured with a gamma camera or simple probe. This clearance technique is currently being used to evaluate patients who have developed pulmonary edema and also to detect those patients from a high risk group who are likely to develop adult respiratory distress syndrome (ARDS). Its role in the evaluation of patients with pulmonary edema is still under active investigation. It is clear that a single measurement in patients who smoke is not useful, but repeated measurements may provide important information. The lung clearance measurement is very sensitive to changes in epithelial integrity but is not specific for ARDS. It may be most useful in combination with other predictive tests or when the clearance rate is normal. 54 references.

  1. Effects of positive expiratory pressure on pulmonary clearance of aerosolized technetium-99m-labeled diethylenetriaminepentaacetic acid in healthy individuals.

    Science.gov (United States)

    Albuquerque, Isabella Martins de; Cardoso, Dannuey Machado; Masiero, Paulo Ricardo; Paiva, Dulciane Nunes; Resqueti, Vanessa Regiane; Fregonezi, Guilherme Augusto de Freitas; Menna-Barreto, Sérgio Saldanha

    2016-01-01

    To evaluate the effects of positive expiratory pressure (PEP) on pulmonary epithelial membrane permeability in healthy subjects. We evaluated a cohort of 30 healthy subjects (15 males and 15 females) with a mean age of 28.3 ± 5.4 years, a mean FEV1/FVC ratio of 0.89 ± 0.14, and a mean FEV1 of 98.5 ± 13.1% of predicted. Subjects underwent technetium-99m-labeled diethylenetriaminepentaacetic acid (99mTc-DTPA) radioaerosol inhalation lung scintigraphy in two stages: during spontaneous breathing; and while breathing through a PEP mask at one of three PEP levels-10 cmH2O (n = 10), 15 cmH2O (n = 10), and 20 cmH2O (n = 10). The 99mTc-DTPA was nebulized for 3 min, and its clearance was recorded by scintigraphy over a 30-min period during spontaneous breathing and over a 30-min period during breathing through a PEP mask. The pulmonary clearance of 99mTc-DTPA was significantly shorter when PEP was applied-at 10 cmH2O (p = 0.044), 15 cmH2O (p = 0.044), and 20 cmH2O (p = 0.004)-in comparison with that observed during spontaneous breathing. Our findings indicate that PEP, at the levels tested, is able to induce an increase in pulmonary epithelial membrane permeability and lung volume in healthy subjects. Avaliar os efeitos da pressão expiratória positiva (PEP) na permeabilidade da membrana epitelial pulmonar em indivíduos saudáveis. Foi avaliada uma coorte de 30 indivíduos saudáveis (15 homens e 15 mulheres), com média de idade de 28,3 ± 5,4 anos, média da relação VEF1/CVF de 0,89 ± 0,14 e média de VEF1 de 98,5 ± 13,1% do previsto. Os indivíduos foram submetidos a cintilografia pulmonar por inalação de radioaerossol de ácido dietilenotriaminopentacético marcado com tecnécio-99m (99mTc-DTPA em inglês) em dois estágios: durante respiração espontânea e durante respiração com uma máscara de PEP de 10 cmH2O (n = 10), 15 cmH2O (n = 10) ou 20 cmH2O (n = 10). O 99mTc-DTPA foi nebulizado por 3 min, e sua depuração foi registrada por cintilografia por um

  2. Estimating GFR in children with 99mTc-DTPA renography: a comparison with single-sample 51Cr-EDTA clearance

    DEFF Research Database (Denmark)

    Gutte, Henrik; Møller, Michael L; Pfeifer, Andreas K

    2010-01-01

    study was to evaluate the accuracy of this non-invasive method in children. We calculated GFR from (99m)Tc-diethylene triamine pentaacetic acid (DTPA) renography and compared with (51)Cr-EDTA plasma clearance of 29 children between the age of 1 month and 12 years (mean 4.7 years). The correlation...... between (99m)Tc-DTPA renography and (51)Cr-EDTA plasma clearance was for all children R = 0.96 (n = 29, P...

  3. Estimating glomerular filtration rate in oncology patients receiving Cisplatin chemotherapy: Predicted creatinine clearance against 99mTc-DTPA methods

    Science.gov (United States)

    Khaidah Syed Sahab, Sharifah; Manap, Mahayuddin; Hamzah, Fadzilah

    2017-05-01

    The therapeutic potential of cisplatin as the best anticancer treatment for solid tumor is limited by its potential nephrotoxicity. This study analyses the incidence of cisplatin induced nephrotoxicity in oncology patients through GFR estimation using 99mTc-DTPA plasma sampling (reference method) and to compare with predicted creatinine clearance and Tc-99m renal scintigraphy. A prospective study of 33 oncology patients referred for GFR estimation in Penang Hospital. The incidence of cisplatin induced nephrotoxicity was analysed via radionuclide and creatinine based method. Of 33 samples, only 21 selected for the study. The dose of cisplatin given was 75 mg/m2 for each cycle. The mean difference of GFR pre and post chemotherapy (PSC 2) was 13.38 (-4.60, 31.36) ml/min/1.73m2 (p 0.136). Of 21 patients, 3 developed severe nephrotoxicity (GFR < 50ml/min/1.73 m2) contributing 14.3% of incidence. Bland-Altman plot showed only PSC 1 is in agreement with PSC 2 technique. Intraclass Correlation Coefficients (ICC) also showed that PSC 1 has high degree of reliability in comparison to PSC 2 (p < 0.001). The other methods do not show reliability and agreement in comparison to PSC 2 (p < 0.05). 3 of 21 patients (14.3%) developed severe nephrotoxicity post cisplatin chemotherapy. This percentage is much less than the reported 20 - 25% of cases from other studies, probably due to small sample size and biased study population due to strict exclusion criteria. Radionuclide method for evaluating GFR is the most sensitive method for the detection of cisplatin induced nephrotoxicity by showing 3 of 21 patients developing severe nephrotoxicity. PSC 1 was found to be a reliable substitute of PSC 2. The other methods are not reliable for detection of early nephrotoxicity. We will recommend the use of single plasma sampling method (PSC 1) for GFR estimation in monitoring post cisplatin chemotherapy patients.

  4. Accurate and precise plasma clearance measurement using four 99mTc-DTPA plasma samples over 4 h

    OpenAIRE

    Wanasundara, Surajith N.; Wesolowski, Michal J.; Barnfield, Mark C.; Waller, Michael L.; Murray, Anthony W.; Burniston, Maria T.; Babyn, Paul S.; Wesolowski, Carl A.

    2015-01-01

    Objectives Glomerular filtration rate can be measured as the plasma clearance (CL) of a glomerular filtration rate marker despite body fluid disturbances using numerous, prolonged time samples. We desire a simplified technique without compromised accuracy and precision. Materials and methods We compared CL values derived from two plasma concentration curve area methods – (a) biexponential fitting [CL (E2)] and (b) Tikhonov adaptively regularized gamma variate fitting [CL (Tk-GV)] – for 4 vers...

  5. 99mTc-HEPIDA hepatic clearance as a diagnostic tool: usefulness of plasma and hepatic clearance for assessment of hepatic parenchyma performance.

    Science.gov (United States)

    Frieske, Izabela; Surma, Marian J; Bieńkiewicz, Małgorzata; Białkowska-Warzecha, Jolanta; Liniecki, Julian; Kuydowicz, Jan; Kuśmierek, Jacek

    2003-01-01

    Plasma clearance of (99m)Tc-HEPIDA (Cl(Pl)) has been used for two decades for assessment of liver function in patients with diseases of this organ. A specific determination of (99m)Tc-HEPIDA liver clearance (Cl(Hp)) has been developed that provides more direct possibility to evaluate performance of liver parenchyma. Both tests have been studied in healthy volunteers of varying age (48 individuals) and in 83 patients with varying degree of liver damage. The liver damage has been evaluated on the basis of 5 biochemical tests (AspAT, ALAT, GGTP, bilirubine serum concentration, proteinogram) and a score system used for total impairment, which was calculated for each patient. Normal range of Cl(Pl) and Cl(Hp) was determined from a study on healthy individuals (volunteers). The results seem independent of age, but show sex differences. The following values (mean +/- SD) of Cl(Hp) were found in males and females of: (181 +/- 31) ml//min/1.73 m(2) and (158 +/- 22) ml/min/1.73m(2), and of Cl(Pl) were (224 +/- 33) ml/min/1.73 m(2) and (202 +/- 25) ml/min/1.73 m(2) respectively. Accepted lower boundaries of both quantities (mean -2SD) are 115 ml/min/1.73 m(2) and 150 ml/min/1.73 m(2) correspondingly. Negative correlation of individual values of both clearances in all patients with individual score of liver damage were highly significant and correlation coefficients obtained were higher for Cl(Hp) (r = -0.63) than those for Cl(Pl) (r= -0.56). Factorial analysis was performed with the intention of seeing which of the studied factors had the highest factor loading for parenchyma performance that was assumed as the common factor responsible for correlations. The highest value was obtained for hepatic clearance (Cl(Hp)) of (99m)Tc-HEPIDA. In conclusion this quantity seems highly promising as a clinically useful test for assessment of liver performance, both in screening for liver damage and for monitoring of organ conditions during therapy and follow-up of patients.

  6. Tc-99m MDP bone clearance - a new technique designed to evaluate bone metabolism on a quantitative basis. Tc-99m-MDP Knochenclearance, ein neues Verfahren zur quantitativen Beurteilung des Knochenstoffwechsels

    Energy Technology Data Exchange (ETDEWEB)

    Fegert, J.

    1984-09-18

    A new technique for the quantitative evaluation of bone metabolism (bone clearance) is described, the diagnostic value of which at the hospital level is rated higher than that of qualitative bone scanning, as it permits metabolic imbalances in the bone to be ascertained at an earlier stage and to reduce the radiation exposure of the patients and hospital staff. Bone clearance was expressed as the ratio between the per minute accumulation of activity in the bone and the plasma activity per ml and calculated on the basis of the measured elimination rate of 99m-Tc MDP from the blood and soft tissues. The latter, in turn, was determined by subtraction of the glomerular filtration rate from the wholebody retention of 113mIn DTPA. The results obtained were corrected for the relative amounts of radioactivity bound to plasma proteins. Considerably increased clearance values were determined for patients showing metabolic or systemic disorders and, in particular, thyrotoxicosis (normal thyroid findings in the scintigrams) as well as in cases of premature ossification of epiphyseal cartilages in a growing organism. Markedly decreased clearance values were seen in patients receiving cortisone. The measured values were easily reproducible and consistent with the relevant hormone levels, while being completely independent of the renal function. (TRV).

  7. Improved renal clearance and tumor targeting of {sup 99m}Tc-labeled anti-Tac monoclonal antibody Fab by chemical modifications

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Meyoung-kon; Jeong, Hyeh-Jean; Kao, Chih-Hao K.; Yao, Zhengsheng; Paik, David S.; Pie, Jae Eun; Kobayashi, Hisataka; Waldmann, Thomas A.; Carrasquillo, Jorge A.; Paik, Chang H. E-mail: cpaik@mail.cc.nih.gov

    2002-02-01

    This study was undertaken to improve the renal clearance and tumor targeting properties of {sup 99m}Tc-labeled humanized anti-Tac (HuTac) monoclonal antibody Fab fragments using two chemical approaches: 1) labeling with a renal secretion agent {sup 99m}Tc-mercaptoacetyltriglycine (MAG3) and 2) lowering its isoelectric point (pI) by acylation. HuTac Fab (3.3 mg/mL) was reacted with a trifluorophenyl ester (TFP) of {sup 99m}Tc-MAG3 alone or was additionally reacted with TFP-glycolate to reduce the pI. In Balb/c mice, {sup 99m}Tc-MAG3-Fab (pI>9.3) rapidly accumulated in the kidneys (177% injected dose [ID]/g at 15 min) and then gradually cleared out of the kidneys. In contrast, the glycolation (pI 4.6{approx}6.6) drastically reduced the renal uptake (31% ID/g) and also the whole-body retention (82% ID vs 101% for the nonglycolated) at 15 min, indicating that the glycolated {sup 99m}Tc-MAG3-Fab (pI 4.6{approx}6.6) was rapidly excreted. The glycolated remained in the blood longer than the nonglycolated (1.2% vs 0.3% ID/g at 360 min), but this effect was less drastic than the effect shown on the renal uptake. In nude mice bearing receptor-positive (ATAC4) tumors, the glycolated {sup 99m}Tc-MAG3-Fab increased the peak tumor uptake to 14.8% ID/g from 8.3% ID/g for {sup 99m}Tc-MAG3-Fab, whereas the glycolation resulted in a drastic reduction of the renal uptake at 15 min. We demonstrated that the renal clearance and the tumor targeting of Fab could be optimized by chemical modifications.

  8. Biological effects in lymphocytes irradiated with {sup 99m}Tc: determination of the curve dose-response; Efeitos biologicos em linfocitos irradiados com {sup 99m}Tc: determinacao da curva dose-resposta

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Romero Marcilio Barros Matias de

    2002-08-01

    Biological dosimetry estimates the absorbed dose taking into account changes in biological parameters. The most used biological indicator of an exposition to ionizing radiation is the quantification of chromosomal aberrations of lymphocytes from irradiated individuals. The curves of dose versus induced biological effects, obtained through bionalyses, are used in used in retrospective evaluations of the dose, mainly in the case of accidents. In this research, a simple model for electrons and photons transports was idealized to simulate the irradiation of lymphocytes with {sup 99m} Tc, representing a system used for irradiation of blood cells. The objective of the work was to establish a curve of dose versus frequencies of chromosomal aberrations in lymphocytes of human blood. For the irradiation of blood samples micro spheres of human serum of albumin (HSAM) market with {sup 99m} Tc were used, allowing the irradiation of blood with different administered activities of {sup 99m} Tc, making possible the study the cytogenetical effects as a function of such activities. The conditions of irradiation in vivo using HSAM spheres marked with {sup 99m} Tc were simulated with MCNP 4C (Monte Carlo N-Particle) code to obtain the dose-response curve. Soft tissue composition was employed to simulate blood tissue and the analyses of the curve of dose versus biological effect showed a linear quadratic response of the unstable chromosomal aberrations. As a result, the response of dose versus chromosomal aberrations of blood irradiation with {sup 99m} Tc was best fitted by the curve Y=(8,99 {+-}2,06) x 1-{sup -4} + (1,24 {+-}0,62) x 10{sup -2} D + (5,67 {+-} 0,64) x 10{sup -2} D{sup 2}. (author)

  9. Detection of impaired renal function. Is the modern serologic marker cystatin C more accurate than the {sup 99m}Tc-MAG{sub 3} clearance?

    Energy Technology Data Exchange (ETDEWEB)

    Reinhardt, M.J.; Weidling, H.; Breuel, H.P.; Biersack, H.J. [Klinik und Poliklinik fuer Nuklearmedizin, Univ. Bonn (Germany)

    2004-12-01

    Aim: Real function is usually determined by means of creatinine-clearance, and of serum Cystatin C, the latter with increasing frequency. The present study analyses, whether the diagnostic accuracy of {sup 99m}Tc-MAG{sub 3} clearance is comparable to that of these modern serologic methods. Patients, methods: 71 consecutive adult Caucasian patients (42 female, 29 male; age 50{+-}16 yrs., range 20-83) who were referred to a nuclear medicine department for determination of bilateral renal function with {sup 99m}Tc-MAG{sub 3} were included. Following sufficient hydration, 10 ml of blood were taken for determination of Cystatin C and creatinine in serum prior to i.v. injection of the radiotracer. According to the recommendations of the National Kidney Foundation, glomerular filtration rate (GFR) was calculated form serum creatinine using either Cockcroft and Gault and Modification of Diet in Renal Disease (MDRD) study equation. These estimates of GFR served as reference. Cystatin C is a low molecular protein produced by all nuclear cells and is eliminated to 85% by glomerular filtration. Analysis of {sup 99m}Tc-MAG{sub 3} clearance was performed by means of Bubeck's formula. Results: Linear regression analysis produced Pearson's correlation coefficients of r=0.68 and r=-0.69 for the comparison of either Cystatin C and {sup 99m}Tc-MAG{sub 3} clearance with the Cockcroft and Gault equation. The comparison of Cystatin C and {sup 99m}Tc-MAG{sub 3} clearance with MDRD study equation resulted in correlation coefficients of r=0.755 and r=-0.77. None of these differences were significant. The exclusion of renal impairment or the detection of an at least moderate renal impairment revealed again no significant differences between Cystatin C and {sup 99m}Tc-MAG{sub 3} clearance. Conclusions: Cystatin C and {sup 99m}Tc-MAG{sub 3} clearance are equally suited to exclude renal impairment or to detect a relevant renal impairment. Differences between both procedures are more

  10. [Radioisotopic quantification of kidney function using Tc-99m-DMSA. Comparison with creatinine clearance in children with a single kidney].

    Science.gov (United States)

    Baillet, G; Gagnadoux, M F; Mathonnat, F; de Vernejoul, P; Broyer, M

    1986-01-01

    To assess the accuracy of renal function quantification with Tc 99m-DMSA in children, we compared DMSA renal uptake and creatinine clearance in 16 cases of children with single kidney. The age of the patients ranged from two month to fourteen years. DMSA renal uptake was measured 7 hours after injection and was normalized in percent of the injected activity. A significant correlation was found between creatinine clearance and DMSA uptake (Pearson's r = 0.866, p less than 0.01). Normal creatinine clearance in children (80 to 120 ml/min-1 X 1.73 m-2) allowed determination of normal renal uptake (36 to 60%). This study indicates that in cases of asymmetrical renal impairment renal uptake reflects split renal creatinine clearance. Since the former is much easier to measure, DMSA should play an important role in the evaluation of differential renal function.

  11. Assessment of sup 99m Tc-DMSA renoscintigraphy and uptake compared with creatinine clearance in rats with drug-induced nephrotoxicity, 2; Cisplatin-induced nephrotoxicity

    Energy Technology Data Exchange (ETDEWEB)

    Yamada, Masafumi (Ehime Univ., Shigenobu (Japan). School of Medicine)

    1991-04-01

    For evaluation of technetium-99m dimercaptosuccinic acid ({sup 99m}Tc-DMSA) renal uptake as an absolute renal function, {sup 99m}Tc-DMSA uptake was compared with endogenous creatinine clearance (Ccr) in cisplatin-induced nephrotoxicity. At first, male Wistar rats were given intraperitoneally 1.8 mg/kg/day of cisplatin for periods of 3, 5, 7 and 9 days. On the next day, {sup 99m}Tc-DMSA uptake and Ccr were measured. Ccr of 5-day treated group was significantly lower than that of control (0.13+-0.10 vs 0.34+-0.05 ml/min/100 g; p<0.01) but {sup 99m}Tc-DMSA uptake did not change. {sup 99m}Tc-DMSA uptake of 7-day treated group was significantly lower than that of control (28.57+-7.23 vs 39.84+-2.23%; p<0.01). As the second experiment, cisplatin (3.6 mg/kg/day) was given intraperitoneally on the 1st, 2nd, 15th and 16th day. On the 5th, 8th, 11th, 15th, 20th, 23rd, 26th and 30th day, the same measurements were done as the first one. Ccr was lower in cisplatin treated rats on the 5th day than that in control (0.10+-0.03 vs 0.34+-0.05 ml/min/100 g; p<0.01), thereafter tended to be recovered to the control level. On the other hand, {sup 99m}Tc-DMSA uptake was lower than that of control on the 8th, 11th and 15th day (32.40+-3.86, 32.56+-1.19, 35.21+-2.97 vs 39.84+-2.23%, respectively; p<0.01). The discrepancy between {sup 99m}Tc-DMSA uptake and Ccr was observed in the cisplatin-induced nephrotoxicity. {sup 99m}Tc-DMSA uptake was suggested to be a reliable indicator of a renal function in a different way from Ccr. (author).

  12. Effects of positive expiratory pressure on pulmonary clearance of aerosolized technetium-{sup 99m}-labeled diethylenetriaminepentaacetic acid in healthy individuals

    Energy Technology Data Exchange (ETDEWEB)

    Albuquerque, Isabella Martins de, E-mail: albuisa@gmail.com [Universidade Federal de Santa Maria (UFSM), Santa Maria, RS (Brazil). Departamento de Fisioterapia e Reabilitacao; Cardoso, Dannuey Machado; Paiva, Dulciane Nunes [Universidade de Santa Cruz do Sul, RS (Brazil); Masiero, Paulo Ricardo; Menna-Barreto, Sergio Saldanha [Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre (Brazil); Resqueti, Vanessa Regiane; Fregonezi, Guilherme Augusto de Freitas [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil)

    2016-11-15

    Objective: To evaluate the effects of positive expiratory pressure (PEP) on pulmonary epithelial membrane permeability in healthy subjects. Methods: We evaluated a cohort of 30 healthy subjects (15 males and 15 females) with a mean age of 28.3 ± 5.4 years, a mean FEV{sub 1}/FVC ratio of 0.89 ± 0.14, and a mean FEV{sub 1} of 98.5 ± 13.1% of predicted. Subjects underwent technetium-99m labeled diethylenetriaminepentaacetic acid ({sup 99m}TcDTPA) radio aerosol inhalation lung scintigraphy in two stages: during spontaneous breathing; and while breathing through a PEP mask at one of three PEP levels—10 cmH{sub 2}O (n = 10), 15 cmH{sub 2}O (n = 10), and 20 cmH{sub 2}O (n = 10). The {sup 99m}Tc-DTPA was nebulized for 3 min, and its clearance was recorded by scintigraphy over a 30-min period during spontaneous breathing and over a 30-min period during breathing through a PEP mask. Results: The pulmonary clearance of {sup 99m}Tc-DTPA was significantly shorter when PEP was applied—at 10 cmH{sub 2}O (p = 0.044), 15 cmH{sub 2}O (p = 0.044), and 20 cmH{sub 2}O (p = 0.004) - in comparison with that observed during spontaneous breathing. Conclusions: Our findings indicate that PEP, at the levels tested, is able to induce an increase in pulmonary epithelial membrane permeability and lung volume in healthy subjects. (author)

  13. Clearance of inhaled technetium-99m-DTPA as a clinical index of pulmonary vascular disease in systemic sclerosis

    Energy Technology Data Exchange (ETDEWEB)

    Kon, O.M.; Daniil, Z.; Bois, R.M. du [Royal Brompton Hospital, Interstitial Lung Disease Unit, London (United Kingdom); Black, C.M. [Royal Free Hospital, Dept. of Rheumatology, London (United Kingdom)

    1999-01-01

    This study evaluated the utility of the clearance time of inhaled diethylenetriamine pentaacetate (DTPA) to distinguish pulmonary vascular disease from early fibrosing alveolitis (FA) in patients with systemic sclerosis (SSc) It was hypothesized that this would be preserved in patients with vascular disease compared with FA, despite similar gas-transfer deficits and matching lung volumes, because of the preservation of alveolar epithelial integrity. All patients had SSc and were categorized into a control group (C; n=9), pulmonary vascular group (VAS; n=14) or FA group (n=14) dependent on the appearance on a computed tomography (CT) scan and the transfer factor of the lung for carbon monoxide (TL,CO) (VAS and FA {<=}70%, C {>=}80%). All patients had a forced vital capacity (FVC) of >80%. The TL,CO (median) was similar in the VAS (57.5%) and FA (60%) groups. There was a significant difference in median DTPA clearance half-times between FA (21.25 min) and VAS (46.5 min) (p=0.014) and between FA and C (84.5 min) (p=0.0004). No difference was found between VAS and C (p=0.0778). Follow-up data from the VAS group showed no subsequent development of FA on the CT scan and no decrease in FVC (n=13, mean 42 months). These results suggest that clearance of diethylenetriamine pentaacetate is preserved in patients likely to have pulmonary vascular disease and may be useful in distinguishing fibrosing alveolitis from vascular disease in systemic sclerosis. (au) 22 refs.

  14. Comparison of the Glomerular Filtration Rate Estimated by Equation with That Assayed with Isotope 99m Tc-DTPA Clearance%核素99mTc-DTPA测定GFR与公式估算GFR的相关性研究

    Institute of Scientific and Technical Information of China (English)

    俞传琪; 蒋更如

    2008-01-01

    目的:探讨核素99mTc-DTPA测定GFR与MDRD公式、简化MDRD公式估算GFR的相关性.方法:178例CKD患者,用核素99mTc-DTPA测定GFR,同时测定血清肌酐、尿素氮和白蛋白,根据年龄和性别分别用MDRD和简化MDRD公式估算GFR.结果:核素99mTc-DTPA测定的GFR与MDRD公式估算的GFR有显著性差异,P=0.0001, MDRD公式高估了GFR;核素99mTc-DTPA测定的GFR与简化MDRD公式估算的GFR亦有显著性差异,P=0.0001,简化MDRD公式低估了GFR; MDRD公式估算的GFR与核素99mTc-DTPA测定的GFR呈正相关,r=0.8292, P<0.01;简化MDRD公式估算的GFR与核素99mTc-DTPA测定的GFR值呈正相关,r=0.8277, P<0.01.结论:MDRD公式、简化MDRD公式估算的GFR与核素99mTc-DTPA测得的GFR有差异,需进一步校正后用于估算GFR.

  15. Assessment of the arterial input curve for [99mTc]-d,l-HM-PAO by rapid octanol extraction

    DEFF Research Database (Denmark)

    Andersen, A R; Friberg, H; Lassen, N A

    1988-01-01

    a rapid method for measuring the radiochemical purity (RCP) of lipophilic [99mTc]-d,l-HM-PAO. In saline, the RCP declined with a half-life of more than 1 h. In human plasma and whole blood, the conversion of [99mTc]-d,l-HM-PAO was biexponential due to the differences in the conversion rates of the d and l...... isomeric forms. The initial half-life representing the conversion rate of the l form was 1.7 min in blood and 1.4 min in plasma, while the conversion half-life of the d form was 7.4 and 24.4 min, respectively. In vivo, the RCP of arterial blood sampled after an i.v. bolus injection showed an initial peak...... value of 75% (68-79%) during the initial, first passage of the bolus. It declined to approximately 35% (29-40%) after 1.5 min and reached very low levels (about 1%) at 6 to 10 min. Quantitative measurements of cerebral blood flow using [99mTc]-d,l-HM-PAO necessitates a rapid method for RCP determination...

  16. Application of measuring {sup 99m}Tc-MAG3 plasma clearance based on one-compartment model (MPC method) to pediatric patients

    Energy Technology Data Exchange (ETDEWEB)

    Koizumi, Kiyoshi [Tokyo Medical Coll., Hachioji (Japan). Hachioji Medical Center; Higashida, Kousuke; Arbab, A.S.; Toyama, Keiji; Arai, Takao; Yoshitomi, Tatsuya

    1997-04-01

    Measurement of {sup 99m}Tc-MAG3 plasma clearance based on 1-compartment model (MPC method) were applied to 12 pediatric patients and evaluated for the factors which might affect the calculated results. Depth correction is a critical factor for the measurement of renal uptake. Three different equations for estimating renal depth were compared with the real depth measured by ultrasonography. The equation proposed by K. Itoh was suitable though the equations by T. Ito and Raynaud were insufficient. Estimation of distribution volume, which is regarded as circulating plasma volume (CPV), is also critical for the calculation of MAG3 clearance by MPC method. Precisely, hematocrit measured by venous sampling and circulating blood volume (CBV) calculated as 7.5% of body weight are used for estimation of CPV. However, assumed CPV as 5% of body weight was acceptable if the hematocrit was not severely deviated from the normal value. Simplified MPC method utilizing two factors mentioned above gave a positive correlation with Russell`s one point sampling method. In conclusion, MPC method is applicable for pediatric patients. (author)

  17. Clearance of /sup 99m/-technetium-labelled DTPA in asbestos-exposed subjects without clinical or radiological evidence of interstitial lung disease

    Energy Technology Data Exchange (ETDEWEB)

    Gellert, A.R.; Langford, J.A.; Winter, R.J.; Lewis, C.A.; Tolfree, S.E.; Rudd, R.M.

    1985-01-01

    The half-time clearance of an inhaled aerosol of /sup 99m/-technetium-labelled diethylene triamine pentacetate from lung to blood (T1/2LB) was measured using a gamma camera in 20 non-smoking subjects (mean age 54, range 40-69 years) with previous occupational asbestos exposure, but no clinical or radiological evidence of asbestosis, and 20 non-smoking normal subjects (mean age 54, range 40-62). Mean T1/2LB was 44.7 minutes (range 12-102) in exposed subjects, significantly less than 57.2 minutes (range 30.5-109) in normal subjects (P less than 0.05). There was no correlation between age and T1/2LB in either group. In exposed subjects T1/2LB showed a weak rank correlation with the membrane component of DLco (Dm) (r = 0.40, P less than 0.05) but no significant correlation with FVC, TLC, DLco, Kco, resting Pao2 or change in Pao2 on exercise. In six exposed subjects T1/2LB was shorter than in any of the normal subjects. These six did not differ from the other 14 exposed subjects in any physiological variables. T1/2LB is abnormal in some asbestos-exposed subjects without clinical, radiological or physiological evidence of asbestosis. Follow-up will show whether it is an early indicator of development of interstitial lung disease.

  18. Area between the hepatic and heart curves of (99m)Tc-galactosyl-human serum albumin scintigraphy represents liver function and disease progression for preoperative evaluation in hepatocellular carcinoma patients.

    Science.gov (United States)

    Harada, Kohei; Mizuguchi, Toru; Katagiri, Yoshimi; Kawamoto, Masaki; Nakamura, Yukio; Meguro, Makoto; Ota, Shigenori; Sasaki, Shigeru; Miyanishi, Koji; Sonoda, Tomoko; Mori, Mitsuru; Shinomura, Yasuhisa; Kato, Junji; Hirata, Koichi

    2012-11-01

    We developed software to calculate the pixels of interest in the area between the hepatic and heart curves (ABC) of (99m)Tc-galactosyl human serum albumin (GSA) scintigraphy. The aim of this study was to examine the accuracy of the ABC to evaluate liver function before hepatectomy. Between January 2005 and December 2010, 205 consecutive patients who underwent initial hepatectomy were enrolled in this study. The ABC was calculated using original computer software. The area under the receiver operating characteristic curve (AUC) was calculated for evaluation of Child-Pugh score grade B (Child B), pathological chronic hepatitis (CH), and liver cirrhosis (LC). The AUC of any indicator for Child B was more than 0.900 except bilirubin. The AUC of ABC for CH and LC (AUC 0.734 each) was comparable to those of HH15 (clearance index; AUC 0.704 and 0.700, respectively) and LHL15 (receptor index; AUC 0.703 and 0.706, respectively) in multiple receiver operating characteristic comparison. We have developed a novel liver function indicator, the ABC, to count radioactivity in sequence. The ABC reflects liver function according to pathological deterioration of the liver. Although the ABC gave no significant advantage compared to HH15 and LHL15, it improved the AUC evaluation by 0.028-0.034.

  19. Technetium-99m tetrofosmin imaging in patients with subacute thyroiditis

    Energy Technology Data Exchange (ETDEWEB)

    Hiromatsu, Yuji; Miyake, Ikuyo; Nonaka, Kyohei [Div. of Endocrinology and Metabolism, Kurume Univ. School of Medicine, Kurume (Japan); Ishibashi, Masatoshi [Division of Nuclear Medicine, Kurume Univ. School of Medicine, Kurume (Japan)

    1998-10-01

    We studied the significance of technetium-99m tetrofosmin scintigraphy in patients with subacute thyroiditis (SAT). Six patients with SAT, who had painful goitre with thyrotoxicosis, underwent {sup 99m}Tc-pertechnetate scintigraphy and {sup 99m}Tc-tetrofosmin imaging during the acute and recovery stages of SAT. The thyroid uptake ratio of tetrofosmin was compared with the clinical parameters associated with SAT. {sup 99m}Tc-pertechnetate scintigraphy showed markedly reduced uptake in the thyroid during the acute stage of SAT, suggesting that the appropriate metabolic pathway is not functioning. Conversely, {sup 99m}Tc-tetrofosmin images showed diffuse increased uptake in the thyroid region on early and delayed imaging. Tetrofosmin images in the acute stage and in the recovery stage of SAT showed different clearance curves for tetrofosmin uptake. The uptake ratio assessed as thyroid uptake/background (T/B) correlated with the serum C-reactive protein concentration. In conclusion, {sup 99m}Tc-tetrofosmin uptake may reflect the inflammatory process associated with SAT, and thus this tracer may have potential as a marker of disease activity and severity. (orig.) With 3 figs., 1 tab., 23 refs.

  20. Labeling of creatinine with technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Yurt Lambrecht, F. [Ege Univ., Bornova, Izmir (Turkey). Dept. of Nuclear Applications, Inst. of Nuclear Sciences; Durkan, K. [Dokuz Eylul Univ., Buca, Izmir (Turkey). Chemistry Technicianship Program, Izmir Vocational School; Soylu, A. [Dokuz Eylul Univ., Narlidere, Izmir (Turkey). Dept. of Pediatrics, Medical Faculty

    2004-07-01

    Creatinine is a clinically important index of renal glomerular filtration rate. Urine creatinine levels can be used as a screening test to evaluate kidney function or can be part of the creatinine clearance test. In case of kidney dysfunction or muscle disorders the creatinine concentration in serum/plasma may rise to a higher value than in healthy body. Technetium- 99m has been used in nuclear medicine and in biomedical research to label molecular and cellular structures employed as radiotracers. {sup 99m}Tc is utilized to label molecules and cells, used as radiopharmaceuticals, and also to label biological species. It presents many desirable characteristics. SnCl{sub 2} method is frequently used as a reducing agent in the {sup 99m}Tc- labeling process. Creatinine metabolism might be investigated by using labeled {sup 99m}Tc- creatinine in healthy or uremic rats. (orig.)

  1. Technetium-99m generator system

    Science.gov (United States)

    Mirzadeh, S.; Knapp, F.F. Jr.; Collins, E.D.

    1998-06-30

    A {sup 99}Mo/{sup 99m}Tc generator system includes a sorbent column loaded with a composition containing {sup 99}Mo. The sorbent column has an effluent end in fluid communication with an anion-exchange column for concentrating {sup 99m}Tc eluted from the sorbent column. A method of preparing a concentrated solution of {sup 99m}Tc includes the general steps of: (a) providing a sorbent column loaded with a composition containing {sup 99}Mo, the sorbent column having an effluent end in fluid communication with an anion-exchange column; (b) eluting the sorbent column with a salt solution to elute {sup 99m}Tc from the sorbent and to trap and concentrate the eluted {sup 99m}Tc on the ion-exchange column; and (c) eluting the concentrated {sup 99m}Tc from the ion-exchange column with a solution comprising a reductive complexing agent. 1 fig.

  2. Evaluation of lung epithelial permeability in the volatile substance abuse using Tc-99m DTPA aerosol scintigraphy.

    Science.gov (United States)

    Cayir, Derya; Demirel, Koray; Korkmaz, Meliha; Koca, Gokhan

    2011-10-01

    Chronic inhalant use is associated with significant toxic effects, including neurological, renal, hepatic, and pulmonary damage. However, there is a paucity of reports regarding respiratory complications in inhalant abusers. The aim of this study was to evaluate pulmonary epithelial permeability in the volatile substance abuse (VSA) using Tc-99m DTPA aerosol scintigraphy. This study included 18 patients with volatile substance abuse and 18 volunteer controls. All of patients and controls were smokers. Tc-99m DTPA aerosol scintigraphy was performed in all cases. Time-activity curves from each lung were generated and clearance half-time (T(1/2)) of Tc-99m DTPA were calculated. T(1/2) of whole lung was calculated as a mean of the T(1/2) of left and right lung. The T(1/2) values of Tc-99m DTPA clearance in the substance abusers were significantly decreased as compared to the control group with respective mean values of 28.86 ± 8.44, and 62.14 ± 26.12 min (p = 0.001). It was seen Tc-99m DTPA clearance from lung was faster as the duration of substance abuse was increased. Tc-99m DTPA pulmonary clearance is markedly accelerated in the volatile substance abuse. This suggests that inhalant abuse of substance may produce abnormalities in pulmonary alveolo-capillary membrane function.

  3. 三种肾小球滤过率检测方法与99mTc-DTPA清除率的比较与分析%Evaluation on three kinds of glomerular filtration rate and comparison with 99mTc-DTPA clearance

    Institute of Scientific and Technical Information of China (English)

    唐琦; 史浩; 王伟铭; 陈楠; 陈晓农; 徐耀文

    2005-01-01

    目的为临床准确评估肾小球滤过率(GFR)提供一定依据.方法对101例各种慢性肾脏病患者,外周静脉注射非离子型造影剂碘海醇(iohexol)5 ml,4 h后取血2ml,用高效液相色谱(HPLC)法检测血浆iohexol浓度,运用数字模型计算血浆iohexol清除率(iohexol-GFR).同步检测Scr及99mTc-DTPA清除率(99mTc-GFR).应用Cockcroft-Gault(CG)、肾脏病膳食改良试验(MDRD)公式计算Ccr、GFR.以99mTc-GFR为标准,运用相关回归分析及受试者工作曲线(ROC)对上述指标进行比较.结果慢性肾脏病第1期至第5期,iohexol-GFR与99mT-GFR相关系数r分别为0.87、0.89、0.88、0.86、0.87(P均<0.01);iohexol-GFR在ROC曲线下面积平均为0.97.MDRD-GFR与99mTc-GFR相关系数r分别为0.80、0.75、0.71、0.67、0.56(P均<0.01),MDRD-GFR在ROC曲线下面积平均为0.82.CG-Ccr与99mTc-GFR相关系数r分别为0.76、0.67、0.62、0.60、0.53(P均<0.05),CG-Ccr在ROC曲线下面积平均为0.82.结论iohexol-GFR检测GFR的准确性优于MDRD-GFR、CG-Ccr,值得推广应用.在无检测条件的情况下,CG、MDRD公式不失为衡量GFR的良好指标.

  4. sup 99m Tc renal tubular function agents: Current status

    Energy Technology Data Exchange (ETDEWEB)

    Eshima, D.; Fritzberg, A.R.; Taylor, A. Jr. (Emory Univ. School of Medicine, Atlanta, GA (USA))

    1990-01-01

    Orthoiodohippuric (OIH) acid labeled with 131I is a widely used renal radiopharmaceutical agent and has been the standard radiopharmaceutical agent for the measurement of effective renal plasma flow (EPRF). Limitations to the routine clinical use of 131I OIH are related to the suboptimal imaging properties of the 131I radionuclide and its relatively high radiation dose. 123I has been substituted for 131I; however, its high cost and short shelf-life have limited its widespread use. Recent work has centered on the development of a new 99mTc renal tubular function agent, which would use the optimal radionuclidic properties and availability of 99mTc and combine the clinical information provided by OIH. The search for a suitable 99mTc renal tubular function agent has focused on the diamide dithiolate (N2S2), the paraaminohippuric iminodiacetic acid (PAHIDA), and the triamide mercaptide (N3S) donor ligand systems. To date, the most promising 99mTc tubular function agent is the N3S complex: 99mTc mercaptoacetyltriglycine (99mTc MAG3). Studies in animal models in diuresis, dehydration, acid or base imbalance, ischemia, and renal artery stenosis demonstrate that 99mTc MAG3 behaves similarly to 131I OIH. A simple kit formulation is available that yields the 99mTc MAG3 complex in high radiochemical purity. Studies in normal subjects and patients indicate that 99mTc MAG3 is an excellent 99mTc renal tubular agent, but its plasma clearance is only 50% to 60% that of OIH. In an effort to develop an improved 99mTc renal tubular function agent, changes have been made in the core N3S donor ligand system, but to date no agent has been synthesized that is clinically superior to 99mTc MAG3. 61 references.

  5. 计算法与应用99mTc-DTPA清除率测定肾小球滤过率的临床对比研究%The clinical contrast study of determining glomerular filtration rate by calculation and using clearance rate of99mTc-DTPA

    Institute of Scientific and Technical Information of China (English)

    吴锡信; 陈江林; 彭健

    2002-01-01

    目的评价临床应用计算法测定肾小球滤过率(GFR)的准确性.方法用99mTc-DTPA清除率测定60例不同疾病住院病人GFR(Tc-GFR),并测血清肌酐,同时以WCP公式测定GFR(WCP-GFR),以Cockcroft/Gault公式计算内生肌酐清除率(CG-CCr).肾功能不全及正常组WCP-GFR、CG-GFR分别与Tc-GFR进行相关分析,比较两组WCP-GFR、CG-GFR与Tc-GFR间显著性差异以及WCP-GFR、Tc-GFR间平均差和CG-GFR、Tc-GFR间平均差的显著性差异.结果 WCP-GFR及CG-CCr均可在一定程度上准确反映GFR,而以WCP-GFR数值更接近Tc-GFR,准确性更高,临床上可代替Tc-GFR.结论 WCP-GFR是一种准确、简便、快捷测定GFR方法.

  6. In vivo hepatic uptake inhibition study of Tc-99m-labeled hepatobiliary agents

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Meyoung Kon [National Institutes of Health, Bethesda (United States)

    1998-07-01

    We have previously reported that the hepatic uptake of Tc-99m-mercaptoacetyltriglycine (MAG3)-biocytin was not affected by coinjecting bilirubin in mice whereas the uptake of Tc-99m-diisopropyliminodiacetic acid (DISIDA) was inhibited (Kim et al. J Nucl Med, 38 : 50 p, 1997). The aim of this study was to investigate whether the hepatic uptake of Tc-99m-MAG3-biocytin or Tc-99m-DISIDA could be inhibited by biotin and biocytin. The Balb/c mice (female, 20g, n=5-8) were injected i.v. with the hepatobiliary agents alone (15-22 MBq/20-40 {mu}g) or together with inhibitors at two doses (14 and 28 mg/kg). For pharmacokinetic studies, images were acquired at 10-sec intervals for 20 min using a gamma camera equipped with a pin-hole collimator (d= 1 mm), starting immediately after intravenous injection. Pharmacokinetic parameters, peak liver/heart ratio (Rmax) and hepatic half clearance time (HCT), were calculated from liver and heart time-acitivity curves from regions-of-interest. Dynamic images showed rapid hepatic uptake inhibition was characterized by persistent high blood background. These qualitative scintigraphic findings were reflected in the pharmacokinetic parameters. Tc-99m-MAG3-biocytin without inhibitor coinjection showed Rmax of 9.3 and HCT of 383 sec. These parameters did not change significantly when bilirubin or biotin was coinjected, but did change significantly (P<0.05) for biocytin only at the higher dose: 52% decrease in HCT. In contrast, the parameters for Tc-99m-DISIDA (Rmax of 9.2 and HCT of 258 sec) were greatly affected (P<0.01) by biotin (79% decrease in Rman and 2-fold increase in HCT) even at 14 mg/kg concentration. We concluded that Tc-99m-MAG3-biocytin is less sensitive to inhibition by bilirubin, biotin, and biocytin than Tc-99m-DISIDA. Tc-99m-MAG3-biocytin appears to be a promising hepatobiliary imaging agent for hepatic function studies and may also be a useful tool to investigate the hepatic uptake mechanism of biotin derivatives in vivo.

  7. Biokinetics of {sup 99m}Tc-UBI 29-41 in humans

    Energy Technology Data Exchange (ETDEWEB)

    Melendez-Alafort, Laura; Rodriguez-Cortes, Jeannette; Ferro-Flores, Guillermina E-mail: gff@nuclear.inin.mx; Arteaga De Murphy, Consuelo; Herrera-Rodriguez, Ruth; Mitsoura, Eleni; Martinez-Duncker, Carlos

    2004-04-01

    Antimicrobial peptides have been proposed as new agents to distinguish between bacterial infections and sterile inflammatory processes. {sup 99m}Tc-UBI labeled by a direct method has shown high in vitro and in vivo stability, specific uptake at the site of infection, rapid background clearance, minimal accumulation in non-target tissues and rapid detection of infection sites in mice. The aim of this study was to establish a {sup 99m}Tc-UBI biokinetic model and evaluate its feasibility as an infection imaging agent in humans. Whole-body images from 6 children with suspected bone infection were acquired at 1, 30, 120, 240 min and 24 h after {sup 99m}Tc-UBI administration. Regions of interest (ROIs) were drawn around source organs (heart, liver, kidneys and bladder) on each time frame. The same set of ROIs was used for all 6 scans and the cpm of each ROI were converted to activity using the conjugate view counting method. Counts were corrected by physical decay and by the background correction factor derived from preclinical phantom studies. The image sequence was used to extrapolate {sup 99m}Tc-UBI time-activity curves in each organ and calculate the cumulated activity (A-tilde). Urine samples were used to obtain the cumulative percent of injected activity (% I.A.) versus time renal elimination. The absorbed dose in organs was evaluated according to the general equation described in the MIRD formalism. In addition, {sup 67}Ga-citrate images were obtained from all the patients and used as a control. Biokinetic data showed a fast blood clearance with a mean residence time of 0.52 h. Approximately 85% of the injected activity was eliminated by renal clearance 24 h after {sup 99m}Tc-UBI administration. Images showed minimal accumulation in non-target tissues with an average target/non-target ratio of 2.18 {+-} 0.74 in positive lesions at 2 h. All infection positive{sup 99m}Tc-UBI images were in agreement with those obtained with {sup 67}Ga-citrate. The mean radiation

  8. Novel (99m)Tc(III) Complexes [(99m)TcCl(CDO)(CDOH)2B-R] (CDOH2 = Cyclohexanedione Dioxime) Useful as Radiotracers for Heart Imaging.

    Science.gov (United States)

    Liu, Min; Fang, Wei; Liu, Shuang

    2016-11-16

    In this study, we evaluated seven new (99m)Tc(III) complexes [(99m)TcCl(CDO)(CDOH)2B-R] ((99m)Tc-2Fboroxime: R = 2-formylfuran-3-yl (2F); (99m)Tc-3Fboroxime: R = furan-3-yl (3F); (99m)Tc-5Fboroxime: R = 5-formyfuran-2-yl (5F); (99m)Tc-HPboroxime: R = 6-hydroxylpyridin-2-yl (HP); (99m)Tc-MPYboroxime: R = 5-methoxypyridin-3-yl (MPY); (99m)Tc-PMboroxime: R = 1,5-pyrimidin-3-yl (PM); and (99m)Tc-4PYboroxime: R = pyridin-4-yl (4PY)) for their potential as heart imaging agents. All new (99m)Tc(III) radiotracers except (99m)Tc-2Fboroxime were prepared with high radiochemical purity (RCP > 95%). The low RCP (∼75%) for (99m)Tc-2Fboroxime is most likely caused by steric hindrance from the 3-formyl group. Biodistribution and imaging studies were performed in SD rats. Planar image quantification was performed to compare their myocardial retention times. We found that the myocardial washout curves of new (99m)Tc(III) radiotracers were best fitted the biexponential decay function. The AUC (area under the curve) values followed the general trend: (99m)Tc-5Fboroxime (129 ± 6) > (99m)Tc-3Fboroxime (114 ± 11) > (99m)Tc-Teboroxime (104 ± 16) > (99m)Tc-MPYboroxime (92 ± 18) > (99m)Tc-4PYboroxime (77 ± 10) > (99m)Tc-PMboroxime (68 ± 14) ≈ (99m)Tc-HPboroxime (62 ± 14). The 2 min heart uptake values from biodistribution studies follow the ranking order of (99m)Tc-5Fboroxime (3.75 ± 0.15%ID/g) ≈ (99m)Tc-MPYboroxime (3.73 ± 0.24%ID/g) > (99m)Tc-PMboroxime (3.47 ± 0.15%ID/g) ≈ (99m)Tc-3Fboroxime ≈ (3.25 ± 0.77%ID/g). The 5 min heart uptake of (99m)Tc-5Fboroxime (3.91 ± 0.09%ID/g) was almost identical to its 2 min heart uptake (3.75 ± 0.15%ID/g), and its 15 min heart uptake value (2.83 ± 0.08%ID/g) compared well to the 2 min heart uptake of (99m)Tc-Teboroxime (3.00 ± 0.37%ID/g). It took ∼5 min for (99m)Tc-5Fboroxime to approach the 1 min heart uptake value of (99m)Tc-Teboroxime (∼3.5% ID/g) and ∼9.5 min to reach the 2 min heart uptake value of (99m

  9. Technetium-99m in production and use

    Directory of Open Access Journals (Sweden)

    Vučina Jurij

    2009-01-01

    Full Text Available Several types of generators have been developed for the production of 99mTc. Due to its excellent performances, the chromatographic type, based on the fission-produced 99Mo sorbed in alumina, is predominant. Technetium-99m is obtained in the form of sodium pertechnetate-99mTc. However, due to the known disadvantages of the production of (n, f99Mo, attempts are made to avoid uranium fission. The technologies based on (n, g99Mo (sublimation, extraction, gel are, with the exception of the gel generator, of limited importance. Certain nuclear reactions in cyclotrons can produce 99Mo (or directly 99mTc but the obtained results are still not satisfying. Technetium-99m is used in the form of radiopharmaceuticals which are prepared by addition of 99mTc-eluate to the inactive components comprised in the 'cold' kits. The chromatographic (n, f99Mo/99mTc generator and several 99mTc-radiopharmaceuticals have been developed and are regularly produced in the Vinča Institute of Nuclear Sciences (Laboratory for Radioisotopes.

  10. Cyclotron Production of Technetium-99m

    Science.gov (United States)

    Gagnon, Katherine M.

    Technetium-99m (99mTc) has emerged as the most widely used radionuclide in medicine and is currently obtained from a 99Mo/ 99mTc generator system. At present, there are only a handful of ageing reactors worldwide capable of producing large quantities of the parent isotope, 99Mo, and owing to the ever growing shutdown periods for maintenance and repair of these ageing reactors, the reliable supply 99mTc has been compromised in recent years. With an interest in alternative strategies for producing this key medical isotope, this thesis focuses on several technical challenges related to the direct cyclotron production of 99mTc via the 100Mo(p,2n)99mTc reaction. In addition to evaluating the 100Mo(p,2n)99mTc and 100Mo(p,x)99Mo reactions, this work presented the first experimental evaluation of the 100Mo(p,2n) 99gTc excitation function in the range of 8-18 MeV. Thick target calculations suggested that large quantities of cyclotron-produced 99mTc may be possible. For example, a 6 hr irradiation at 500 μA with an energy window of 18→10 MeV is expected to yield 1.15 TBq of 99mTc. The level of coproduced 99gTc contaminant was found to be on par with the current 99Mo/99mTc generator standard eluted with a 24 hr frequency. Highly enriched 100Mo was required as the target material for 99mTc production and a process for recycling of this expensive material is presented. An 87% recovery yield is reported, including metallic target preparation, irradiation, 99mTc extraction, molybdate isolation, and finally hydrogen reduction to the metal. Further improvements are expected with additional optimization experiments. A method for forming structurally stable metallic molybdenum targets has also been developed. These targets are capable of withstanding more than a kilowatt of beam power and the reliable production and extraction of Curie quantities of 99mTc has been demonstrated. With the end-goal of using the cyclotron-produced 99mTc clinically, the quality of the cyclotron

  11. Comparative scanning of thyroid nodules with technetium-99m pertechnetate and technetium-99m methoxyisobutylisonitrile

    Energy Technology Data Exchange (ETDEWEB)

    Foeldes, I.; Levay, A. (Central Hospital of the Ministry of the Interior, Budapest (Hungary). Dept. of Nuclear Medicine); Stotz, G. (Central Hospital of the Ministry of the Interior, Budapest (Hungary). Dept. of Pathology)

    1993-04-01

    Thyroid imaging was performed using technetium-99m methoxyisobutylisonitrile and technetium-99m pertechnetate in 58 patients. The [sup 99m]Tc-pertechnetate scans showed a total of 77 nodules: 60 cold, 13 hot and 4 of normal acitivity. There was no [sup 99m]Tc-MIBI accumulation in 46.4% of [sup 99m]TC-pertechnetate cold nodules; 27 (45%) of these nodules showed [sup 99m]TC-MIBI uptake with the same intensity as the surrounding normal tissue, and five (8.6%) became hot with [sup 99m]Tc-MIBI. Of the [sup 99m]TC-pertechnetate hot nodules 11 (84.6%) could not be differentiated from the normal extranodular tissue on the [sup 99m]Tc-MIBI scan. The histopathology of 34 surgically removed nodules proved that increased, normal or decreased [sup 99m]Tc-MIBI accumulation is not specific for thyroid malignance and that the [sup 99m]Tc-MIBI uptake depends mainly on the viability of thyroid tissue. (orig.).

  12. Technetium-99m DTPA inhalation scintigraphy in patients treated with fluoxetine and maprotiline: preliminary results

    Energy Technology Data Exchange (ETDEWEB)

    Kaya, G.C.; Durak, H.; Oezdogan, Oe.; Sayit, E.; Degirmenci, B.; Derebek, E. [Dokuz Eyluel Univ., Izmir (Turkey). School of Medicine; Yemez, B.; Turhal, Ue. [Dept. of Psychiatry, Dokuz Eylul Univ. School of Medicine, Izmir (Turkey)

    2000-09-01

    Drug-metabolising enzymatic activities have been detected in tracheobronchiolar, bronchiolar and alveolar regions in the lungs. Induction of phospholipidosis by amine drugs such as clorphentermine has also been shown. This study aimed to investigate the effect of fluoxetine and maprotiline, which contain amine groups in their structure, on pulmonary epithelial membrane permeability. Twenty-seven patients (mean age 36{+-}12 years) with various psychiatric problems, of whom 17 were treated with fluoxetine and 10 with maprotiline, were included in this study. Technetium-99m diethylene triamine penta-acetic acid (DTPA) aerosol inhalation scintigraphy was performed before and after 4-6 weeks of therapy. Following the inhalation of 1480 MBq {sup 99m}Tc-DTPA for 3 min, lung images in a 64 x 64 matrix were obtained every minute for 30 min. Regions of interest were drawn around the periphery of the lungs and on the major airways. Clearance half-times (T{sub 1/2}) were calculated by placing a mono-exponential fit on the curves. Penetration index (PI) was calculated on the first-minute image. There was no difference between the clearance rates of {sup 99m}Tc-DTPA before and after therapy for either the fluoxetine or the maprotiline group. After therapy, a significant decrease in PI was found in patients treated with fluoxetine (PI values before and after therapy: 0.53{+-}0.03 and 0.49{+-}0.05 respectively, P{<=}0.05). This finding might have been due to the induction of increased synaptic serotonin (5-HT) by fluoxetine, which acts by inhibiting the re-uptake of 5-HT on presynaptic membranes. Bronchoconstriction of small and medium airways may be caused by direct and indirect effects of 5-HT on smooth muscle contraction. (orig.)

  13. Evaluation of residual functional lung volume on Tc-99m DTPA aerosol ventilation and Tc-99m MAA perfusion scintigraphy in primary ciliary dyskinesia (Kartagener syndrome).

    Science.gov (United States)

    Chen, Yu-Wen; Chang, Chin-Chuan; Lai, Yung-Chuang; Lu, Chia-Ying; Dai, Zen-Kong

    2008-12-01

    Kartagener syndrome is diagnosed as sinusitis, bronchitis (bronchiectasis), and situs inversus by the clinical features. It is a subclass of primary ciliary dyskinesia (PCD) disease. A 12-year-old girl who had frequent upper and lower airway infections since birth, which was confirmed as Kartagener syndrome by HRCT imaging. We present the residual functional lung volume and mucociliary clearance findings seen on Tc-99m DTPA aerosol ventilation and Tc-99m MAA perfusion scintigraphy.

  14. Determination of the mean transit time for the transport of aerosolized 99mTc-DTPA across the pulmonary epithelial membrane. A plasma sample method

    DEFF Research Database (Denmark)

    Groth, Sofie; Lassen, N A; Rossing, Niels Nygaard

    1988-01-01

    During the last decade it has been customary to estimate pulmonary epithelial permeability (P-P) as the pulmonary clearance of inhaled nebulized 99mTc-DTPA from a time-activity (t-a) curve registered externally by monitoring over the chest. The t-a curve, however, is not defined by the degree of P......-P alone but also by factors such as the deposition of the 99mTc-DTPA in the lungs. To avoid these problems a plasma sample method was derived. It describes P-P by the mean transit time (t) for the transport of 99mTc-DTPA across the pulmonary epithelial membrane, t(L). The calculation of t(L) involves two...... steps. Following the inhalation of Tc-DTPA the plasma t-a curve is defined and used to calculate t for the transport for 99mTc-DTPA across the pulmonary epithelial membrane, through the ECV and until elimination by the kidneys, t(L+ECV). Subsequently, 99mTc-DTPA is injected as a bolus i.v. and the new t...

  15. Technetium-99m ceftizoxime kit preparation

    Directory of Open Access Journals (Sweden)

    Simone Odília Fernandes Diniz

    2005-10-01

    Full Text Available The aim of this work was to prepare a kit of 99mTc-ceftizoxime (99mTc-CFT, with stability and biological activity preserved, able to identify a septic focus (E. coli in the experimental infection model in rats. The preparation of the CFT kit involved the use of lyophilized solutions containing the antibiotic ceftizoxime and the sodium dithionite reducing agent (6.0 mg/mL. After lyophilization, the kit was reconstituted with 1.0 mL of sodium 99mTc-pertechnetate solution (Na99mTcO4- with an activity of 370 MBq. The solution was boiled for 10 min and filtered through a cellulose ester filter. The labeling efficiency was on the order of 92%, remaining stable for six hours and the kit remained stable for two months. The biological activity of the 99mTc-CFT was evaluated by diffusion in agar impregnated with E.coli and S. aureus. Seven Wistar rats, weighing from 200 to 250 g, were used for the development of the septic focus. After 24 hours from the induction of the infectious site (E.coli, the animals were anesthetized and 0.1 mL of 99mTc-CFT (37 MBq was injected into the tail veins of the animals. The images were obtained with a gamma camera one, two and six hours after injection and the regions of interest (ROIs were calculated. The diameters of the inhibition halos for 99mTc-CFT were 27.16 ± 0.23 and 27.17 ± 0.20 for S.aureus and E.coli, respectively, while those for the unlabeled CFT were 30.4 ± 0.33 and 29.43 ± 0.26, respectively. The results for the biodistribution of 99mTc-CFT in infected animals furnished a ratio of 1.97 ± 0.31, 2.10 ± 0.42 and 2.01 ± 0.42 for cpm-target/cpm-no target for the one, two and six-hour periods, respectively. The images showed a clear uptake of labeled antibiotic (99mTc-CFT by the infectious site during the experiment. The results attest to the viability of producing a kit with 99m technetium-labeled ceftizoxime for the investigation of infectious processes.O objetivo deste trabalho foi preparar um kit de Tc

  16. Sclerosing cholangitis: cholescintigraphy with Tc-99m-labeled DISIDA

    Energy Technology Data Exchange (ETDEWEB)

    Ament, A.E.; Bick, R.J.; Miraldi, F.D.; Haaga, J.R.; Wiedenmann, S.D.

    1984-04-01

    Four patients with primary sclerosing cholangitis (PCS) were examined with the hepatobiliary agent Tc-99m-labeled DISIDA (diisopropylphenylcarbamoyl iminodiacetic acid), and the results correlated with those of invasive cholangiography. Cholescintigraphy is superior to cholangiography in cases of suspected PSC where there is nonfilling of biliary radicals due to high-grade stenosis. The finding of delayed hepatic parenchymal clearance can allow estimation of the degree of obstruction of the various branches of the major bile ducts. Cholescintigraphy offers a noninvasive method of investigating patients with suspected sclerosing chloangitis, leading to earlier diagnosis. Confirmation with invasive cholangiographic procedures is recommended.

  17. Direct 99m Tc labeling of Herceptin (trastuzumab) by 99m Tc(I) tricarbonyl ion.

    Science.gov (United States)

    Chen, Wan-Jou; Yen, Chao-Liang; Lo, Su-Tang; Chen, Kuo-Ting; Lo, Jem-Mau

    2008-03-01

    By simply incubating Herceptin (trastuzumab) with [99m Tc(CO)3(OH2)3]+ ion in saline, a significant yield of 99m Tc-labeled trastuzumab was found to be achievable. The effective labeling may be based on that trastuzumab is inherent with endogenous histidine group to which 99m Tc(I) tricarbonyl ion can be strongly bound. For practical 99m Tc labeling processing, trastuzumab was purified beforehand from the commercial product, Herceptin (Genentech) via size exclusion chromatography to remove the excipient, alpha-histidine and a high-labeled yield could be obtained by incubating the purified trastuzumab with [99m Tc(CO)3(OH2)3]+. Retention of bioactivity of the 99m Tc(I)-labeled trastuzumab was validated using a cell binding test.

  18. Experimental studies on imaging of infected site with 99mTc-Iabeled ciprofloxacin in mice

    Institute of Scientific and Technical Information of China (English)

    ZHANG Hong; JIANG Ning-yi; ZHU Lin

    2009-01-01

    Background Bacterial infection can pose a substantial diagnostic dilemma.99mTc-labeled ciprofloxacin (CPF) was developed as a biologically active radiopharmaceutical to diagnose infection.In the present research,we studied the biodistribution and imaging properties of infection tracer 99mTc-CPF in a mouse model of infection.Methods CPF was labeled with 99mTc and the radiochemical purity and labeling rate were measured.A mouse model of infection was established.We then determined the biodistribution of 99mTC-CPF and conducted the whole body scintigraphy of the animal model.Results 99mTc-Ciprotech was stable for at least 6 hours at room temperature.The labeling rate of CPF by 99mTc was over 90%.Clearance of radioactivity mainly occurred in the liver and kidney,and the clearance from blood was rapid.Both biodistribution and imaging results showed higher uptake of 99mTc-CPF at sites of infection.The infectious tissue/normal tissue ratio peak was 4.30 at 4 hours after injection.Conclusions 99mTc-CPF is a sensitive radiopharmaceutical for scintigraphy of infectious lesions and it is easy to prepare.

  19. A kinetic model for 99mTc-DMSA in the rat.

    Science.gov (United States)

    Maneval, D C; D'Argenio, D Z; Wolf, W

    1990-01-01

    A pharmacokinetic model was developed for the renal imaging agent 99mTc-DMSA in anesthetized rats, which incorporated data from serial measurements of blood and urine simultaneously with dynamic images obtained over an 8-h period. Animals which received a 10 mg/kg dose of unlabeled DMSA immediately before 99mTc-DMSA injection had a significantly reduced kidney accumulation and greater urinary elimination of 99mTc than animals which received the radiopharmaceutical alone. The kidney clearance was also significantly lower in rats receiving unlabeled DMSA, but no significant difference was determined between the urine clearance estimates of the two animal groups. Because the increase in the amount eliminated in the urine was not coupled with a significant change in urine clearance, it would appear that unlabeled DMSA saturated the kidney uptake mechanism(s) of 99mTc-DMSA without modifying the urinary clearance process. This interpretation is consistent with the hypothesis that renal handling of 99mTc-DMSA is governed by both glomerular filtration and peritubular capillary uptake. The simultaneous acquisition of blood, urine and non invasive image data allows for a comprehensive and informative model of the physiological disposition of 99mTc-DMSA.

  20. Dynamic coupling of {sup 99m}Tc-MIBI efflux and apoptotic pathway activation in untreated breast cancer patients

    Energy Technology Data Exchange (ETDEWEB)

    Del Vecchio, Silvana; Zannetti, Antonella; Aloj, Luigi; Fonti, Rosa; Salvatore, Marco [Nuclear Medicine Center of the National Research Council (CNR) and Department of Biomorphological and Functional Sciences University ' ' Federico II' ' , Naples (Italy); Ciarmiello, Andrea; Botti, Gerardo; D' Aiuto, Giuseppe [National Cancer Institute, Via M. Semmola, Naples (Italy); Caraco, Corradina [Institute of Experimental Medicine and Biotechnologies, CNR, Piano Lago Mangone, Cosenza (Italy)

    2002-06-01

    Our previous studies showed that the efflux rate of technetium-99m methoxyisobutylisonitrile (MIBI) is directly correlated to P-glycoprotein (Pgp) levels in breast carcinoma. The aim of this study was to test whether the Pgp-dependent efflux of {sup 99m}Tc-MIBI is related to the apoptotic pathway activation in breast carcinoma. Thirty-three untreated non-consecutive patients were intravenously injected with 740 MBq {sup 99m}Tc-MIBI and serial images were obtained up to 4 h. The rate of efflux was determined by mono-exponential fitting of decay-corrected time-activity curves. Tumour specimens were then obtained at surgery and processed for the determination of the apoptotic index by in situ end-labelling of DNA fragments (Tunel). The rate of tumour cell proliferation was also determined using Ki67 monoclonal antibody. All breast carcinomas showed focal uptake of {sup 99m}Tc-MIBI and the time to half clearance varied between 85 and 574 min. The apoptotic index ranged between 0.3% and 4.2%, whereas the rate of proliferation varied between 13% and 40%. We found a positive and significant correlation between the apoptotic index and the rate of proliferation (r=0.79, P<0.0001). The efflux rate of {sup 99m}Tc-MIBI was directly and significantly correlated with the apoptotic index (r=0.74, P<0.0001) and with the rate of proliferation (r=0.58, P<0.001). After partial correlation analysis, only the apoptotic index showed a significant correlation with the efflux rate of {sup 99m}Tc-MIBI (r=0.57, P<0.001). Our findings indicate that enhanced transport activity of Pgp is associated with increased activation of the apoptotic pathway, suggesting that inhibition of Pgp function with specific modulators may be effective in these patients. Furthermore, since mitochondria are central executioners of apoptosis and intracellular sites of {sup 99m}Tc-MIBI sequestration, a model for the dynamic coupling of Pgp-dependent {sup 99m}Tc-MIBI efflux and apoptotic pathway activation may be

  1. {sup 99m}Tc-glucarate kinetics differentiate normal, stunned, hibernating, and nonviable myocardium in a perfused rat heart model

    Energy Technology Data Exchange (ETDEWEB)

    Okada, David R. [University of Pennsylvania School of Medicine, Philadelphia, PA (United States); Liu, Zhonglin [University of Arizona School of Medicine, Tucson, AZ (United States); Johnson, Gerald; Okada, Robert D. [University of Oklahoma Health Sciences Center, Oklahoma, OK (United States); University of Tulsa, Tulsa, OK (United States); Beju, Delia [Oklahoma State University School of Medicine, Tulsa, OK (United States); Khaw, Ban An [Northeastern University, Boston, MA (United States)

    2010-10-15

    {sup 99m}Tc-glucarate is an infarct-avid imaging agent. However, patients may have mixtures of normal, irreversibly injured, stunned, and hibernating myocardium. The purposes were to determine {sup 99m}Tc-glucarate uptake and clearance kinetics in these four conditions, and its ability to determine the extent of injury. Twenty-two perfused rat hearts were studied: controls (n = 5), stunned (n = 5; 20-min no-flow followed by 5-min reflow), hibernating (n = 6; 120-min low flow at 4 ml/min), and ischemic-reperfused (n = 6; 120-min no-flow followed by reflow). {sup 99m}Tc-glucarate was then infused. Tracer activity was monitored using a NaI scintillation detector and a multichannel analyzer. Creatine kinase, electron microscopy, and triphenyltetrazolium chloride determined viability. {sup 99m}Tc-glucarate 10-min myocardial uptake was significantly greater in ischemic-reperfused (2.50 {+-} 0.09) (cpm, SEM) than in control (1.74 {+-} 0.07), stunned (1.68 {+-} 0.11), and hibernating (1.59 {+-} 0.11) (p < 0.05). Tracer retention curves for ischemic-reperfused were elevated at all time points as compared with the other groups. {sup 99m}Tc-glucarate 60-min myocardial uptake was significantly greater in ischemic-reperfused (7.60 {+-} 0.63) than in control (1.98 {+-} 0.15), stunned (1.79 {+-} 0.08), and hibernating (2.33 {+-} 0.15) (p < 0.05). The 60-min well-counted tracer activity ratio of ischemic-reperfused to control was 9:1 and corroborated the NaI detector results. Creatine kinase, triphenyltetrazolium chloride, and electron microscopy all demonstrated significantly greater injury in ischemic-reperfused compared to the other groups. An excellent correlation was observed between viability markers and tracer activity (r = 0.99 triphenyltetrazolium chloride; r = 0.90 creatine kinase). {sup 99m}Tc-glucarate activity continually and progressively increased in irreversibly injured myocardium. {sup 99m}Tc-glucarate uptake was strongly correlated with myocardial necrosis as

  2. Clinical need for both scintigraphy with technetium-99m GSA and per-rectal portal scintigraphy in some patients with chronic liver disease

    Energy Technology Data Exchange (ETDEWEB)

    Shiomi, Susumu; Iwata, Yoshinori; Sasaki, Nobumitsu [Osaka City Univ. (Japan). Medical School] (and others)

    1999-08-01

    Scintigraphy with {sup 99m}Tc-diethylenetriaminepentaacetate with galactosyl human serum albumin ({sup 99m}Tc-GSA) and per-rectal portal scintigraphy are useful for evaluating hepatic functional reserve and portal circulation, respectively. We did the procedures simultaneously in some patients to examine the relationship between hepatic functional reserve and portal circulation in chronic liver disease. Scintigraphy with {sup 99m}Tc-GSA was done in 10 healthy subjects, 45 patients with chronic hepatitis, and 165 patients with cirrhosis. Fifty-seven patients (13 with hepatitis and 44 with cirrhosis) also underwent per-rectal portal scintigraphy with {sup 99m}Tc-pertechnetate within two weeks. A receptor index was calculated by dividing the radioactivity of the liver region of interest (ROI) by that of the liver-plus-heart ROI at 15 min after the injection of {sup 99m}Tc-GSA. The index of blood clearance was calculated by dividing the radioactivity of the heart ROI at 15 min by that of the heart ROI at 3 min. A solution containing {sup 99m}Tc-pertechnetate was instilled into the rectum, and serial scintigrams were taken while radioactivity curves for the liver and heart were recorded sequentially. A per-rectal portal shunt index was determined by calculating the ratio of counts for the liver to counts for the heart integrated for 24 seconds immediately after the appearance of the liver time-activity curve. The median receptor index was lower for more severe liver disorders, increasing in the order of chronic hepatitis, compensated cirrhosis and decompensated cirrhosis, and the median index of blood clearance was higher. The median receptor index was significantly lower when a complication (varices, ascites, or encephalopathy) was present, and the median index of blood clearance was higher. The shunt index was correlated significantly with the two other indices, but these values for some one-third of the patients disagreed in either indices. Scintigraphy with {sup 99m

  3. A novel {sup 99m}Tc-labeled dimethyl-substituted zoledronic acid (DMIDP) with improved bone imaging efficiency

    Energy Technology Data Exchange (ETDEWEB)

    Qiu, L.; Lin, J.; Luo, S.; Wang, Y.; Cheng, W.; Zhang, S. [Jiangsu Institute of Nuclear Medicine, Wuxi (China). Key Laboratory of Nuclear Medicine

    2012-07-01

    A novel zoledronic acid (ZL) derivative, 1-hydroxy-2-(2,4-dimethyl-1H-imidazol-1-yl)-ethane-1,1-diyldiphosphonic acid (DMIDP, dimezoledronate), was successfully prepared and labeled with {sup 99m}Tc in a high labeling yield. The biodistribution in mice shows that {sup 99m}Tc-DMIDP has significant advantage regarding bone resorption and clearance from soft tissues compared with {sup 99m}Tc-ZL and the clinically widely-used bone-imaging agent {sup 99m}Tc-MDP (methylenediphosphonic acid). Kinetics of blood clearance displayed that distribution half-life (T{sub 1/2{alpha}}) and elimination half-life (T{sub 1/2{beta}}) of {sup 99m}Tc-DMIDP were 2.53 min and 23.53 min, while those of {sup 99m}Tc-ZL were 2.28 and 52.63 min, respectively. Excellent images of the rabbit skeleton can be quickly obtained from {sup 99m}Tc-DMIDP, which was faster than {sup 99m}Tc-ZL and {sup 99m}Tc-MDP. The present findings indicate that {sup 99m}Tc-DMIDP possesses excellent potential for application as a novel bone scanning agent. (orig.)

  4. Glomerular filtration rate estimated from the uptake phase of 99mTc-DTPA renography in chronic renal failure

    DEFF Research Database (Denmark)

    Petersen, L J; Petersen, J R; Talleruphuus, U

    1999-01-01

    The purpose of the study was to compare the estimation of glomerular filtration rate (GFR) from 99mTc-DTPA renography with that estimated from the renal clearance of 51Cr-EDTA, creatinine and urea.......The purpose of the study was to compare the estimation of glomerular filtration rate (GFR) from 99mTc-DTPA renography with that estimated from the renal clearance of 51Cr-EDTA, creatinine and urea....

  5. Glomerular filtration rate estimated from the uptake phase of 99mTc-DTPA renography in chronic renal failure

    DEFF Research Database (Denmark)

    Petersen, L J; Petersen, J R; Talleruphuus, U

    1999-01-01

    The purpose of the study was to compare the estimation of glomerular filtration rate (GFR) from 99mTc-DTPA renography with that estimated from the renal clearance of 51Cr-EDTA, creatinine and urea.......The purpose of the study was to compare the estimation of glomerular filtration rate (GFR) from 99mTc-DTPA renography with that estimated from the renal clearance of 51Cr-EDTA, creatinine and urea....

  6. Standardization of 99mTc.

    Science.gov (United States)

    Sahagia, Maria

    2006-01-01

    The radioactivity of 99mTc was standardized by the 4piPC-gamma coincidence method with two different modes. One is using coincidences between (119.5-142.6)keV conversion electrons and K X-rays, and the other is coincidences between the 2.13 keV conversion electrons and 140.5 keVgamma-rays. The background of the K X-ray peak and the sensitivity of the proportional counter (PC) to 140 keV gamma-rays were the main sources of uncertainties in the first case and low detection efficiency for conversion electrons in the second case. General coincidence equations were written, with specific forms, for the three measurement variants, including literature variant. Comparison with the ionization chamber calibration is reported.

  7. Is the renal uptake of {sup 99m}Tc-DMSA decreased in microalbuminuric diabetic patient?

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Seong Jang; Kim, In Ju; Kim, Yong Ki [College of Medicine, Pusan National Univ., Pusan (Korea, Republic of)

    1999-08-01

    Diabetic nephropathy is the most common cause of end stage renal disease and the incidence is progressively increasing. The aim of this study was to investigate the differences of {sup 99m}Tc-DMSA renal uptake among diabetic patients with normoalbuminuria, microalbuminuria and overt proteinuria, and then to determine the clinical usefulness of {sup 99m}Tc-DMSA in predicting early diabetic nephropathy. {sup 99m}Tc-DMSA scan was performed and a total renal uptake of {sup 99m}Tc-DMSA was measured in 145 diabetic patients. Patients were divided into 3 groups according to the amount of 24 hour urinary albumin excretion as Group I (normoalbuminuria, 74 cases ), Group II (microalbuminuria, 39 cases), and Group III (overt proteinuria, 32 cases). The differences of {sup 99m}Tc-DMSA renal uptake among the 3 groups and the correlation between the renal uptake of {sup 99m}Tc-DMSA and other clinical parameters were analyzed. The total renal uptake of {sup 99m}Tc-DMSA of Group II (40.8{+-}11.0%) was significantly lower than that of Group I (54.4{+-}6.3%, p<0.001). The uptake of Group III (27.7{+-}12.0%) was significantly lower than those of both Group I and Group II (p<0.001). {sup 99m}Tc-DMSA total renal uptakes correlated negatively with serum creatinine level (r=0.629, p<0.001) and positively correlated with creatinite clearance rate (r=0.702, p<0.001). {sup 99m}Tc-DMSA total renal uptake of diabetic patients with microalbuminuria was significantly decreased compared with that of patients of normoalbuminuria. Therefore, {sup 99m}Tc-DMSA scan can be used as a diagnostic study for early detection of the diabetic nephropathy.

  8. Pharmacokinetics evaluation of Technetium{sup 99m} Ethylene-di-cysteine diethyl ester radiopharmaceutical; Avaliacao farmacocinetica do radiofarmaco etilenodicisteina dietil este'-{sup 99m} Tc (ECD-{sup 99m} Tc)

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Elaine Bortoleti de; Carvalho, Olga G. de; Muramoto, Emiko; Almeida, Maria A.T.M. de; Mesquita, Carlos H. de [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)

    1996-07-01

    The biological distribution studies of {sup 99m} Tc-ECD in mice were performed in this work. Using ANACOMP computer program, a composed by seven compatiments was adjusted, characterized by a fast blood clearance, an undirectional renal depuration and intestinal elimination, determined by intense hepato biliary transit. Plasma, bile and urinary samples were analysed by HPLC, showing the in vivo metabolism of the compound. (author)

  9. Diagnosis of pulmonary infections with HIV (+) patients. Brought of aerosol DTPA-Tc99m and of Ga67 citrate. Le diagnostic des infections pulmonaires chez les malades VIH (+). Apport de l'aerosol DTPA-Tc99m et du citrate de Ga67

    Energy Technology Data Exchange (ETDEWEB)

    Banzo, I.; Quirce, R.; Serrano, J.; Jimenez, J.; Tabuenca, O.; Carril, J.M. (Hopital Universitaire Valdecilla, Santander (Spain))

    1993-04-01

    The pulmonary clearance of aerosol DTPA-Tc99m is a technology easy to use, well support by patients with immediate results. With the pneumonia at Pneumocystis Carinii (PPC), the clearance is more sensitive and more specific than the thoracic scintigraphy with Ga67. Used with a thorax radiography, results will lead to three directions: High probability of PPC, di phasic curve and very fast T50, equal inferior to 5,10 mn. Low probability of PPC, monoexponential curve and abnormal thorax radiography or di phasic curve with a value T50 superior to 5,10 mn. With these patients other explorations will be made (Ga67, biopsy) and if possible search pulmonary tuberculosis. Extra pulmonary pathology: monoexponential curve associated with a normal thoracic radiography. 2 figs.

  10. /sup 99m/Tc-aprotinin: A new tracer for kidney morphology and function

    Energy Technology Data Exchange (ETDEWEB)

    Bianchi, C.; Donadio, C.; Tramonti, G.; Lorusso, P.; Bellitto, L.; Lunghi, F.

    1984-01-01

    Aprotinin (Ap), a low molecular weight polyeptide (6500 dalton), is a protease inhibitor which is electively and stably accumulated in the kidney. In 112 adult patients, with either uni- or bilateral renal disease with different degrees of renal impairment (from normal GFR to advanced renal failure), renal scans were performed by means of Ap labelled with /sup 99m/Tc. Highly satisfactory renal scans were obtained in all patients. In 20 patients with renal failure (serum creatinine 1.8 - 8.5 mg/dl, mean 4.7) a comparison was made of the renal scans obtained with /sup 99m/Tc-Ap and with /sup 99m/Tc-DMSA. /sup 99m/Tc-Ap was slightly better than /sup 99m/Tc-DMSA, especially in patients with far advanced renal failure. Some aspects of the pharmacokinetics of /sup 99m/Tc-Ap were studied in 72 cases. In 22 of these patients plasma clearance of /sup 99m/Tc-Ap was determined by the single injection method using a two-compartment model. In patients with GFR>90 ml/min plasma cl of /sup 99m/Tc-Ap was 67.6 +- 8.4 SD ml/min. A good correlation was observed between plasma clearance of /sup 99m/Tc-Ap and GFR (r = 0.74). After i.v. injection /sup 99m/Tc-Ap was stably fixed by the kidney. Renal radioactivity remained stable between the 2nd and the 8th hour after the injection. Urinary excretion of radioactivity measured in 35 patients in the first and in the second 2-hour interval after i.v. injection of /sup 99m/Tc-Ap was negligible in all patients (2.7 +- 1.5 SD percent of the dose in the fist 2 hours; 2.8 +- 1.4 SD between the 2nd and the 4th hour). Conclusions. /sup 99m/Tc-Ap is an excellent agent for renal imaging. It also seems promising for renal function studies.

  11. Tc-99m erythromycin lactobionate inhalation scintigraphy in parenchymal lung diseases

    Energy Technology Data Exchange (ETDEWEB)

    Durak, Hatice E-mail: hdurak@kordon.deu.edu.tr; Aktogu, Serir; Degirmenci, Berna; Sayit, Elvan; Ertay, Tuerkan; Dereli, Sevket

    1999-08-01

    We have investigated Technetium 99m erythromycin lactobionate (Tc 99m EL) clearance from the lungs after inhalation, in the presence of an alveolitis. Eighteen patients (6 sarcoidosis, 7 idiopathic fibrosis, and 5 miliary tuberculosis) were imaged after the patients inhaled 1,110 MBq of Tc 99m EL. Clearance half time for the first 45 min, for 24 h, and retention at 24 h correlated with percentage of lymphocytes in bronchoalveolar lavage fluid (BAL) (r=.729, r=.883, and r=.826, respectively). There was a positive correlation between peripheral penetration (PP) and forced expiratory volume in 1 s (FEV{sub 1}) (r=.806) and forced vital capacity (FVC) (r=.781). Retention was more marked in sarcoidosis compared with tuberculosis (0.02599m EL may be related to number of BAL cells or presence of a lymphocytic alveolitis. Long residency time of Tc 99m EL in the lungs implies that erythromycin can also be administered by inhalation for therapeutic purposes.

  12. Biological evaluation of 99mTc-labeled cyclic glycoprotein IIb/IIIa receptor antagonists in the canine arteriovenous shunt and deep vein thrombosis models: effects of chelators on biological properties of [99mTc]chelator-peptide conjugates.

    Science.gov (United States)

    Barrett, J A; Damphousse, D J; Heminway, S J; Liu, S; Edwards, D S; Looby, R J; Carroll, T R

    1996-01-01

    A series of 99mTc-labeled cyclic glycoprotein IIb/IIIa receptor antagonists, [99mTcO(L1-III)]-, [99mTcO-(L6-III)]-, [99mTcO(L1-V)]-, and [99mTcO(L6-V)]-, were evaluated in a canine arteriovenous (AV) shunt model for their potential use as thrombus imaging agents. The thrombus formed consists of a platelet-rich head and a fibrin-rich tail. All four agents were incorporated into the growing thrombus under both arterial (platelet-rich) and venous (platelet-poor) conditions. The rank order for uptake was [99mTcO(L1-V)]- > [99mTcO(L6-V)]- > [99mTcO(L6-III)]- > [99mTcO(L1-III)]- (arterial range, 5.8-0.47% id/g; venous range, 0.58-0.04% id/g). The uptakes of both [99mTcO(L6-III)]- and [99mTcO-(L1-III)]- under both arterial and venous conditions were not significantly greater than that of [99mTc]-albumin and [125I]fibrinogen. In contrast, the uptakes of both [99mTcO(L1-V)]- and [99mTcO(L6-V)]- were significantly greater than those of [99mTc]albumin and [125I]fibrinogen and comparable to that of [111In]platelets under both arterial and venous conditions. All four [99mTc]chelator-peptide conjugates are cleared faster than the controls with the clearance of the conjugates of peptide III faster than that of the conjugates of peptide V. The differences in incorporation are attributable to the effect of both the cyclic peptide and the chelator. The conjugate [99mTcO(L1-V)]- was also studied using a canine DVT (deep vein thrombosis) model. [99mTcO(L1-V)]- was actively incorporated into the growing thrombus with images clearly detectable within 15 min postinjection. At 2 h postinjection, thrombus/blood and thrombus/muscle ratios [region of interest (ROI)/background] were approximately 7/1 and 10/1, respectively. This clearly demonstrated that the conjugate [99mTcO(L1-V)]- has the potential for rapid diagnosis of thrombolic events occurring under both arterial and venous conditions.

  13. Current status of Tc-99m production in Thailand

    Energy Technology Data Exchange (ETDEWEB)

    Charoen, Sakda [Office of Atomic Energy for Peace, Bangkok (Thailand)

    2003-03-01

    Technetium-99m is the workhorse of nuclear medicine and currently accounts for over 80% of all in vivo diagnostic procedures. In Thailand, Office of Atomic Energy for Peace (OAEP) had experiences in production of Technetium-99m by solvent extraction process and alternative technology for Technetium-99m generator based on zirconium molybdate and titanium molybdate gel generators were also studied. The paper describes past experiences and future plan of Technetium-99m production in Thailand. (author)

  14. Novel (99m)Tc(III)-azide complexes [(99m)Tc(N3)(CDO)(CDOH)2B-R] (CDOH2=cyclohexanedione dioxime) as potential radiotracers for heart imaging.

    Science.gov (United States)

    Liu, Min; Zheng, Yumin; Avcibasi, Ugur; Liu, Shuang

    2016-11-01

    In this study, novel (99m)Tc(III)-azide complexes [(99m)Tc(N3)(CDO)(CDOH)2B-R] ((99m)Tc-ISboroxime-N3: R=IS; (99m)Tc-MPboroxime-N3: R=MP; (99m)Tc-PAboroxime-N3: R=PA; (99m)Tc-PYboroxime-N3: R=PY; and (99m)Tc-Uboroxime-N3: R=5U) were evaluated as heart imaging agents. Complexes [(99m)Tc(N3)(CDO)(CDOH)2B-R] (R=IS, MP, PA, PY and 5U) were prepared by ligand exchange between NaN3 and [(99m)TcCl(CDO)(CDOH)2B-R]. Biodistribution and imaging studies were carried out in Sprague-Dawley rats. Image quantification was performed to compare their initial heart uptake and myocardial retention. (99m)Tc-ISboroxime-N3, (99m)Tc-PYboroxime-N3 and (99m)Tc-Uboroxime-N3 were prepared with high RCP (93-98%) while the RCP of (99m)Tc-MPboroxime-N3 and (99m)Tc-PAboroxime-N3 was 80-85%. The myocardial retention curves of (99m)Tc-ISboroxime-N3, (99m)Tc-PYboroxime-N3 and (99m)Tc-Uboroxime-N3 were best fitted to the bi-exponential decay function. The half-time of the fast component was 1.6±0.4min for (99m)Tc-ISboroxime-N3, 0.7±0.1min for (99m)Tc-PYboroxime-N3 and 0.9±0.4min for (99m)Tc-Uboroxime-N3. The 2-min heart uptake from biodistribution studies followed the ranking order of (99m)Tc-ISboroxime-N3 (3.60±0.68%ID/g)>(99m)Tc-PYboroxime-N3 (2.35±0.37%ID/g)≫(99m)Tc-Uboroxime-N3 (1.29±0.06%ID/g). (99m)Tc-ISboroxime-N3 had the highest 2-min heart uptake among (99m)Tc radiotracers revaluated in SD rats. High quality SPECT images were obtained with the right and left ventricular walls being clearly delineated. The best image acquisition window was 0-5min for (99m)Tc-ISboroxime-N3. Both azide coligand and boronate caps had significant impact on the heart uptake and myocardial retention of complexes [(99m)Tc(N3)(CDO)(CDOH)2B-R]. Among the radiotracers evaluated in SD rats, (99m)Tc-ISboroxime-N3 has the highest initial heart uptake with the heart retention comparable to that of (99m)Tc-Teboroxime. (99m)Tc-ISboroxime-N3 is a promising alternative to (99m)Tc-Teboroxime for SPECT MPI. Copyright

  15. Effects of radiofrequency ablation on individual renal function: assessment by technetium-99m mercaptoacetyltriglycine renal scintigraphy.

    Directory of Open Access Journals (Sweden)

    Nasu,Yasutomo

    2006-04-01

    Full Text Available We quantitatively evaluated total and individual renal function by technetium-99m mercaptoacetyltriglycine (Tc-99m MAG3 renal scintigraphy before and after radiofrequency ablation (RFA of renal tumors. Eleven patients who underwent Tc-99m MAG3 renal scintigraphy 1 week before and after RFA were evaluated (7 men and 4 women ; age range : 23-83 years ; mean age : 60.6 years. Five patients had solitary kidneys, and five had normally or minimally functioning contralateral kidneys. One patient had a renal cell carcinoma in the contralateral kidney. One patient with a solitary kidney underwent RFA a second time for a residual tumor. In patients with a solitary kidney, MAG3 clearance decreased after 5 of 6 RFAs, and in patients with a normally functioning contralateral kidney, MAG3 clearance decreased after 4 of 5 RFAs, but no significant differences were observed between before and after treatments. In addition to the total MAG3 clearance, the split MAG3 clearance was evaluated in patients with a normally functioning contralateral kidney. MAG3 clearance decreased in 4 of 5 treated kidneys, while it adversely increased in the contralateral kidneys after 4 of 5 RFAs. No significant differences, however, were observed between before and after treatments. The results of our study revealed no significant differences in sCr, BUN, CCr, or MAG3 clearance between pre- and post-RFA values. These results support data regarding the functional impact and safety of renal RFA in published reports. We evaluated total and individual renal function quantitatively using Tc-99m MAG3 renal scintigraphy before and after treatment. This scintigraphy was very useful in assessing the effects of RFA on renal function.

  16. Mismatched uptake of Tc-99m-ECD and Tc-99m-HMPAO in subacute cerebral infarction: Tc-99m-ECD for viability and Tc-99m-HMPAO for flow restoration

    Energy Technology Data Exchange (ETDEWEB)

    Lee, D. S.; Hyun, I. Y.; Kim, S. K. [College of Medicine, Seoul National Univ., Seoul (Korea, Republic of)] [and others

    1997-07-01

    Tc-99m-HMPAO reflects tissue perfusion but Tc-99m-ECD uptake is affected by tissue viability in addition to tissue perfusion which the varied state of cellular retention of Tc-99m-ECD reflects. Luxuriously perfused area on Tc-99m-HMPAO SPECT implies that this cortex was already reperfused either spontaneously or after thrombolysis and that accompanied paralysis of vascular reactivity in those zones warms progressive deterioration. We tried to find out if we can use sequential Tc-99m-ECD/Tc-99m-HMPAO SPECT to reveal cortical perfusion and severity and range of risky areas of cerbral cortex despite reperfusion in sub-acute infarction. In 13 patients (M ; F =7 : 6, mean age 57 (range: 26-84)) with cortical (n=12) and basal ganglia infarction (1), we performed sequential Tc-99m-ECD/Tc-99m-HMPAO SPECT at the same position. At first, 555 MBq of Tc-99m-ECD was injected and imaged and then 1110 MBq of Tc-99m-HMPAO was injected again and imaged with the patients in situ, and the first image (Tc-99m-ECD) and the subtracted image (2nd- 1st : Tc-99m-HMPAO) were compared slice by slice. Study was done from 3 days to 31 days (16{+-}9) after ictus. Tc-99m-ECD uptake was always less than or equal to Tc-99m-HMPAO uptake at the lesion in all cases. Luxury perfusion was prominent in four patients. Mismatched uptake was found in 10 patients. Severity of mismatch showed diverse spectrum and was ranged from total middle cerebral artery territory (1 case) to peripheral thin zones around infarction (2 cases). The other 7 showed intermediate amount of tissues with mismatch , i.e., Tc-99m-ECD defects where Tc-99m-HMPAO uptake is in part increased, normal or decreased. Upon discharge, patients having more uptake with Tc-99m-ECD predicted improvement. Patients having mismatched uptake went dichotomous way. In conclusion, Tc-99m-ECD/Tc-99m-HMPAO sequential SPECT is feasible and reveal both tissue perfusion (Tc-99m-HMPAO ) and discrepant Tc-99m-ECD uptake probably reflecting viability in acute

  17. Usefulness of renal dynamic function study with [sup 99m]Tc-MAG[sub 3] (mercaptoacetylglycylglycylglycine)

    Energy Technology Data Exchange (ETDEWEB)

    Shirakawa, Seishi; Tamaki, Nagara; Torizuka, Tatsuo; Fujita, Toru; Yano, Shinsuke; Tsuchimochi, Shinsaku; Yonekura, Yoshiharu; Konishi, Junji; Terai, Akito (Kyoto Univ. (Japan). Faculty of Medicine)

    1994-08-01

    The clinical value of a newly developed renography agent [sup 99m]Tc-mercaptoacetylglycylglycylglycine ([sup 99m]Tc-MAG[sub 3]) was assessed in comparison with [sup 123]I-orthoiodohippurate ([sup 123]I-OIH). Clear perfusion images were obtained early after [sup 99m]Tc-MAG[sub 3] administration due to physical advantages of the tracer. A close correlation was observed of T[sub max] and T[sub 1/2] values between [sup 99m]Tc-MAG[sub 3] and [sup 123]I-OIH. However, T[sub 1/2] calculated by [sup 99m]Tc-MAG[sub 3] was significantly longer than that by [sup 123]I-OIH. In addition, the effective renal plasma flow (ERPF) value calculated by the clearance rate of the tracer by Tauxe method (radioactivity in plasma at 43 minutes after tracer administration) was smaller than that by [sup 123]I-OIH. In conclusion, [sup 99m]Tc-MAG[sub 3] is considered as a useful agent for renography. (author).

  18. Novel 99mTc labeled

    Institute of Scientific and Technical Information of China (English)

    FAN; Caiyun

    2006-01-01

    ,Nucl.Med.Biol.,2003,30:273-284.[33]John,C.S.,Lim,B.B.,Geyer,B.C.Et al.,99mTc-labeled σ-receptor-binding complex:Synthesis,characterization,and specific binding to human ductal breast carcinoma (T47D) cells,Bioconj.Chem.,1997,8:304-309.[34]Choi,S-R.,Yang,B.,P(o)ssl,K.Et al.,Development of a Tc-99m labeled sigma-2 receptor-specific ligand as a potential breast tumor imaging agent,Nucl.Med.Biol.,2001,28:657-666.[35]Zhang,Y.,Williams,W.,Torrence-Campbell,C.et al.,Characterization of novel N,N′-disubstituted piperazines as sigma receptor ligands,J.Med.Chem.,1998,41:4950-4957.[36]Maeda,D.N.,Williams,W.,Kim,W.E.et al.,N-arylalkylpi-peridines as high-affinuty sigma-1 and sigma-2 receptor ligands:Phenylpropylamine as potential leads for selective sigma-2 agents,Bioorg.Med.Chem.Lett.,2002,12:497-500.[37]Moore,T.S.,Boyle,M.,Thorn,V.M.et al.,N-substituted derivatives of piperazine and ethylenediamine,Part Ⅰ.The preparation of N-monosubstituted derivatives,J.Chem.Soc.,1929:39.[38]Stewart,H.W.,Turner,R.J.,Denton,J.J.et al.,Experimental chemotherapy of filariasis,Ⅳ.The preparation of derivatives of piperazine,J.Org.Chem.,1948,13:134-143.[39]O'Neil,J.P.,Wilson,S.R.,Katzenellenbogen,J.A.,Preparation and structural characterization of monoamine-monoamide bis(thio) oxo complexes of technetium(V) and rhenium(V),Inorg.Chem.,1994,33:319-323.[40]Bowen,W.D.,Sigma receptors:Recent advances and new clinical potentials,Pharm.Acta Helv.,2000,74:211-218.[41]Deuther-Conrad,W.,Patt,J.T.,Feuerbach,D.et al.,Norchloro-fluoro-homoepibatidine:Specificity to neuronal nicotinic acetylcholine receptor subtypes in vitro,IL Farmaco,2004,59:785-792.[42]Vilner,B.J.,Bowen,W.D.,Modulation of cellular calcium by sigma-2 receptors:Release form intracellular stores in human SK-N-SH neuroblastoma cells,J.Pharmacol.Exp.Ther.,2000,292:900-911.[43]Cheng,Y.,Prusoff,W.H.,Relationship between the inhibition constant (Ki) and the concentration of inhibitor which cause 50% inhibition (IC50) of an enzymatic reaction

  19. Biokinetics and dosimetry in patients of {sup 99m}Tc-HYNIC-Lys{sup 3}-Bombesin: images of GRP receptors; Biocinetica y dosimetria en humanos de {sup 99m}Tc-HYNIC-Lys{sup 3}-Bombesina: imagenes de receptores GRP

    Energy Technology Data Exchange (ETDEWEB)

    Santos C, C. L. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)

    2007-07-01

    The bombesin (BN) receptor subtype 2 (GRP-r) is expressed in several normal human tissues and is over-expressed in various human tumors including breast, prostate, small cell lung and pancreatic cancer. Recently [{sup 99m}Tc]EDDA/HYNIC-Lys{sup 3}-bombesin ({sup 99m}Tc-HYNIC-BN) was reported as a radiopharmaceutical with high stability in human serum, specific cell GRP-r binding and rapid cell internalization. The aim of this study was to evaluate the feasibility of using {sup 99m}Tc-HYNIC-BN to image GRP-r and to assess the radiopharmaceutical biokinetics and dosimetry in 4 breast cancer patients and in 7 healthy women. Methods: Whole-body images were acquired at 20, 90, 180 min and 24 h after {sup 99m}Tc-HYNIC-BN administration. Regions of interest (ROIs) were drawn around source organs on each time frame. The same set of ROIs was used for all 11 scans and the cpm of each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate {sup 99m}Tc-HYNIC-BN time-activity curves in each organ in order to calculate the total number of disintegrations (N) that occurred in the source regions, according with MIRD methodology. N data were the input for the OLINDA/EXM code to calculate internal radiation dose estimates. Results: Images showed a rapid radiopharmaceutical blood clearance with renal excretion as predominant route. {sup 99m}Tc-HYNIC-BN exhibited high in vivo affinity for GRP-r over-expression successfully visualized in cancer mammary glands and well differentiated from the ubiquitous GRP-r expression in normal breast, lungs and airways. There was no statistically significant difference (p > 0.05) in the radiation absorbed doses between cancer patients and healthy women. The average equivalent doses (n=11) for a study using 740 MBq were 24.8 +- 8.8 mSv (kidneys), 7.3 +- 1.8 mSv (lungs), 6.5 +- 4.0 mSv (breast) 2.0 +- 0.3 mSv (pancreas), 1.6 +- 0.3 mSv (liver), 1.2 +- 0.2 mSv (ovaries) and 1.0 +- 0.2 mSv (red

  20. Biokinetics and dosimetry in patients of {sup 99m}Tc-EDDA/HYNIC-Tyr{sup 3}-octreotide prepared from lyophilized kits

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez-Vazquez, Armando [Departamento de Medicina Nuclear, Hospital Militar (Mexico); Facultad de Medicina, Universidad Autonoma del Estado de Mexico (Mexico); Ferro-Flores, Guillermina [Departamento de Materiales Radiactivos, Gerencia de Aplicaciones Nucleares en la Salud, Instituto Nacional de Investigaciones Nucleares, Km. 36.5 Carretera Mexico-Toluca, Ocoyoacac, Estado de Mexico, C.P. 52045 (Mexico)]. E-mail: gff@nuclear.inin.mx; Arteaga de Murphy, Consuelo [Departamento de Medicina Nuclear, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran (Mexico); Gutierrez-Garcia, Zohar [Departamento de Medicina Nuclear, Hospital Militar (Mexico)

    2006-07-15

    {sup 99m}Tc-EDDA/HYNIC-Tyr{sup 3}-octreotide ({sup 99m}Tc-HYNIC-TOC) has shown high in vitro and in vivo stability, rapid background clearance and rapid detection of somatostatin receptor-positive tumors. The aim of this study was to establish a biokinetic model for {sup 99m}Tc-HYNIC-TOC prepared from lyophilized kits, and to evaluate its dosimetry as a tumor imaging agent in patients with histologically confirmed neuroendocrine tumors. Whole-body images from eight patients were acquired at 5, 60, 90, 180 min and 24 h after {sup 99m}Tc-HYNIC-TOC administration obtained from instant freeze-dried kit formulations with radiochemical purities >95%. Regions of interest (ROIs) were drawn around source organs on each time frame. The same set of ROIs was used for all eight scans and the count per minute (cpm) of each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate {sup 99m}Tc-HYNIC-TOC time-activity curves in each organ, to adjust a biokinetic model using the SAAM software, and to calculate the total number of disintegrations (N) that occurred in the source regions. N data were the input for the OLINDA/EXM code to calculate internal radiation dose estimates. Images showed an average tumor/blood (heart) ratio of 4.3{+-}0.7 in receptor-positive tumors at 1 h. The mean radiation absorbed dose calculated for a study using 740 MBq was 24, 21.5, 5.5 and 1.0 mSv for spleen, kidneys, liver and bone marrow respectively and the effective dose was 4.4 mSv.

  1. Reduction-mediated technetium-99m labeling of monoclonal antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Mather, S.J.; Ellison, D. (St. Bartholomews Hospital, London (England))

    1990-05-01

    A simple and generally applicable method for labeling antibodies with technetium-99m ({sup 99m}Tc) is described. Following reduction of intrinsic disulphide bonds, the antibody is labeled with {sup 99m}Tc in the presence of a weak competing ligand methylene diphosphonate. High labeling efficiencies (greater than 97%), in a final labeling step taking only a few minutes, can be routinely obtained with high in-vitro stability over 24 hr. No effect upon antibody reactivity is seen.

  2. Retention of 99mTc-DMSA(III) and 99mTc-nanocolloid in different syringes affects imaging quality.

    Science.gov (United States)

    Bauwens, Matthias; Pooters, Ivo; van der Pol, Jochen; Mottaghy, Felix M; van Kroonenburgh, Marinus

    2014-04-01

    (99m)Tc-dimercaptosuccinic acid [DMSA(III)] and colloidal human serum albumin ((99m)Tc-nanocolloid) are widely used radiopharmaceuticals. Recently, in our institution we encountered image quality problems in DMSA scans after changing the brand of syringes we were using, which triggered us to look into the adsorption properties of syringes from different brands for (99m)Tc-DMSA(III) and (99m)Tc-nanocolloid. We also describe a clinical case in which adsorption of (99m)Tc-DMSA(III) caused inferior imaging quality. DMSA and nanocolloid were labeled with (99m)Tc following manufacturer guidelines. After synthesis, syringes with (99m)Tc-DMSA(III) and (99m)Tc-nanocolloid were stored for 15, 30, 60, and 120 min. We evaluated Luer Lock syringes manufactured by different brands such as Artsana, Henke-Sass-Wolf, B. Braun Medical N.V., CODAN Medizinische Geräte GmbH & Co KG, Becton Dickinson and Company, and Terumo Europe. Adsorption of (99m)Tc-DMSA(III) and (99m)Tc-nanocolloid was acceptably low for all syringes (image quality--for example, in DMSA scans using pediatric radiopharmaceutical doses. It is advised to check the compatibility of any radiopharmaceutical with syringes as an integral part of the quality assurance program.

  3. Tumor targeting with a (99m)Tc-labeled AS1411 aptamer in prostate tumor cells.

    Science.gov (United States)

    Noaparast, Zohreh; Hosseinimehr, Seyed Jalal; Piramoon, Majid; Abedi, Seyed Mohammad

    2015-01-01

    AS1411, a 26-base guanine-rich oligonucleotide aptamer, has high affinity to nucleolin, mainly on tumor cell surfaces. In this study, a modified AS1411 was labeled with (99m)Tc and evaluated as a potential tumor-targeting agent for imaging. The AS1411 aptamer was conjugated with HYNIC and labeled with (99m)Tc in the presence a co-ligand. Radiochemical purity and stability testing of the (99m)Tc-HYNIC-AS1411 aptamer were carried out with thin layer chromatography and a size-exclusion column in normal saline and human serum. Cellular nucleolin-specific binding, cellular internalization in DU-145 cells, as high levels of nucleolin expression, were performed. Additionally, biodistribution in normal mice and DU-145 tumour-bearing mice was assessed. Radiolabeling of the aptamer resulted in a reasonable yield and radiochemical purity after purification. The aptamer was stable in normal saline and human serum, and cellular experiments demonstrated specific binding of the AS1411 aptamer to the nucleolin protein. Based on biodistribution assessment of (99m)Tc-HYNIC-AS1411, rapid blood clearance was seen after injection and it appears that the excretion route was via the urinary system at 1 h post-injection. Tumours also showed a higher accumulation of radioactivity with this labeled aptamer. (99m)Tc-AS1411 can be a potential tool for the molecular imaging of nucleolin-overexpressing cancers.

  4. 99mTc-ciprofloxacin for diagnosis of bacterial infection

    Science.gov (United States)

    Aungurarat, A.; Ngamprayad, T.; Dangprasert, M.; Phumkem, S.; Jowanaridhi, B.

    2015-05-01

    Preparation of 99mTc-ciprofloxacin for diagnosis of bacterial infection was investigated by varying factors which affected this compound. The optimum conditions for preparation of 99mTc-ciprofloxacin and a lyophilized kit for Tc-99m labelling were studied. The results from biodistribution study showed that the percentages of the injected dose per gram tissues of infected area at 1 and 3 hours after injection were around 0.25-0.56. 99mTc-ciprofloxacin was found sterile, pyrogen-free and non-toxic. Radiochemical purity was greater than 90% with greater than 6 hours of stability.

  5. Direct {sup 99m}Tc labeling of Herceptin (trastuzumab) by {sup 99m}Tc(I) tricarbonyl ion

    Energy Technology Data Exchange (ETDEWEB)

    Chen, W.-J.; Yen, C.-L.; Lo, S.-T.; Chen, K.-T. [Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan (China); Lo, J.-M. [Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan (China)], E-mail: jmlo@mx.nthu.edu.tw

    2008-03-15

    By simply incubating Herceptin (trastuzumab) with [{sup 99m}Tc(CO){sub 3}(OH{sub 2}){sub 3}]{sup +} ion in saline, a significant yield of {sup 99m}Tc-labeled trastuzumab was found to be achievable. The effective labeling may be based on that trastuzumab is inherent with endogenous histidine group to which {sup 99m}Tc(I) tricarbonyl ion can be strongly bound. For practical {sup 99m}Tc labeling processing, trastuzumab was purified beforehand from the commercial product, Herceptin (Genentech) via size exclusion chromatography to remove the excipient, {alpha}-histidine and a high-labeled yield could be obtained by incubating the purified trastuzumab with [{sup 99m}Tc(CO){sub 3}(OH{sub 2}){sub 3}]{sup +}. Retention of bioactivity of the {sup 99m}Tc(I)-labeled trastuzumab was validated using a cell binding test.

  6. The radiopharmaceuticals labelled with technetium-99m and the radiopharmacy; Les radiopharmaceutiques marques au technetium-99m et la radiopharmacie

    Energy Technology Data Exchange (ETDEWEB)

    Bodenant, V

    1998-10-01

    In less than fifty years, the place of nuclear medicine is become primordial. Among all the radiopharmaceuticals used in nuclear medicine, the technetium-99m is the most used because of its physico-chemical properties and its great availability with the molybdenum-99m - technetium-99m generator. Since 1992, the radiopharmaceuticals, the packages, the generators are included in the pharmaceutic monopole. They are now under the reliability of the radio-pharmacist. This thesis has for object to introduce these different radiopharmaceuticals labelled with technetium-99m and to show the primordial place of the radio-pharmacist in a service of nuclear medicine. (N.C.)

  7. Sustained availability of 99mTc: possible paths forward.

    Science.gov (United States)

    Pillai, Maroor Raghavan Ambikalmajan; Dash, Ashutosh; Knapp, F F Russ

    2013-02-01

    The availability of (99m)Tc for single-photon imaging in diagnostic nuclear medicine is crucial, and current availability is based on the (99)Mo/(99m)Tc generator fabricated from fission-based molybdenum (F (99)Mo) produced using high enriched uranium (HEU) targets. Because of risks related to nuclear material proliferation, the use of HEU targets is being phased out and alternative strategies for production of both (99)Mo and (99m)Tc are being evaluated intensely. There are evidently no plans for replacement of the limited number of reactors that have primarily provided most of the (99)Mo. The uninterrupted, dependable availability of (99m)Tc is a crucial issue. For these reasons, new options being pursued include both reactor- and accelerator-based strategies to sustain the continued availability of (99m)Tc without the use of HEU. In this paper, the scientific and economic issues for transitioning from HEU to non-HEU are also discussed. In addition, the comparative advantages, disadvantages, technical challenges, present status, future prospects, security concerns, economic viability, and regulatory obstacles are reviewed. The international actions in progress toward evolving possible alternative strategies to produce (99)Mo or (99m)Tc are analyzed as well. The breadth of technologies and new strategies under development to provide (99)Mo and (99m)Tc reflects both the broad interest in and the importance of the pivotal role of (99m)Tc in diagnostic nuclear medicine.

  8. Accumulation of 99m TC-methylene diphosphonate radiotracer in ...

    African Journals Online (AJOL)

    Accumulation of 99m TC-methylene diphosphonate radiotracer in rat's forelimb. ... by gamma camera 2 h after the injection of 99mTc-MDP via the tail vein. ... can be used for survey radioactivity examination in different bones of the forelimb.

  9. Muscle localization of Tc-99m MDP after exertion

    Energy Technology Data Exchange (ETDEWEB)

    Valk, P.

    1984-09-01

    Very high muscle uptake of Tc-99m MDP was seen two days after the start of a program of vigorous weight-lifting exercises. Localization of Tc-99m bone tracer in muscle that has been damaged by exertion may be a more common phenomenon than is recognized at present.

  10. Standardization of a method to calculate absolute renal uptake of {sup 99m} Tc-DMSA in children; Padronizacao do metodo para calculo da captacao renal absoluta do {sup 99m}Tc-DMSA em criancas

    Energy Technology Data Exchange (ETDEWEB)

    Ono, Carla Rachel; Sapienza, Marcelo Tatit; Watanabe, Tomoco; Costa, Paulo Luiz Aguirre; Okamoto, Miriam Roseli Yoshie; Garcez, Alexandre Teles; Buchpiguel, Carlos Alberto [Sao Paulo Univ., SP (Brazil). Hospital das Clinicas. Servico de Medicina Nuclear]. E-mail: crachelo@usp.br; Machado, Beatriz Marcondes; Machado, Marcia Melo Campos [Paulo Univ., SP (Brazil). Faculdade de Medicina. Hospital Universitario; Liberato Junior, Waldyr de Paula

    2006-01-15

    Objective:To standardize a method and determine normal values for absolute renal uptake of 99m Tc-DMSA in children with normal creatinine clearance. Materials and methods: Twenty-two children (between 7 months and 10 years of age; mean 4.5 years) without clinical evidence of renal disease were studied using 99m Tc-DMSA scintigraphy. Eighteen had normal renal ultrasonography, micturating urethrocystography, creatinine clearance and visual interpretation of the scintigraphy with 99m Tc-DMSA. Four children were excluded, one with incomplete creatinine clearance and three due to reduction in the creatinine clearance. Absolute renal uptake of 99m Tc-DMSA (DMSA-Abs) was expressed as the fraction of the administered dose retained by each kidney six hours after administration of the radiopharmaceutical. Results: DMSA-Abs was 21.8 +- 3.2% for the right kidney and 23.1 +-3.3% for the left kidney. There was no correlation between renal uptake and the age groups studied, although there was a tendency to an increase in the creatinine clearance with age. Conclusion: Normal values of DMSA-Abs can be used as an additional parameter for the initial diagnostic evaluation and during follow-up of renal diseases, mainly when bilateral impairment of renal function is suspected or in a patient with a single functioning kidney (in which renal differential function is of limited value). (author)

  11. Intra-arterial injection of Tc-99m MDP

    Directory of Open Access Journals (Sweden)

    Ebru Salmanoglu

    2016-03-01

    Full Text Available Bone scintigraphy is applied to evaluate abnormalities of axial skeleton and appendicular skeleton including osteomyelitis, prosthesis infection, prosthesis loosening, avascular necrosis, stress fracture, bone metastasis. Technetium-99m methylene diphosphonate (Tc-99m MDP is most common used radiopharmaceutical. Injection of Tc-99m MDP should be done intravenous but it can be done intra-arterial, accidentally. After intra-arterial injection of Tc-99m MDP, it cause prominent soft tissue uptake distal to the injection site. This situation can create confusion in the scintigraphic images. Usually, it imitate reflex sympathetic dystrophy, so differential diagnosis is important. The aim of this report is to present the appearance of inadvertent intra-arterial injection of Tc-99m MDP. [Cukurova Med J 2016; 41(0.100: 79-82

  12. Detection of esophageal ulcerations with technetium-99m albumin sucralfate

    Energy Technology Data Exchange (ETDEWEB)

    Goff, J.S.; Adcock, K.A.; Schmelter, R.

    1986-07-01

    Technetium-99m albumin-sucralfate ((/sup 99m/Tc)Su) can be used to demonstrate peptic ulcer disease in man and animals. We evaluated the usefulness of (/sup 99m/Tc)Su for detecting various grades of esophagitis. (/sup 99m/Tc)Su adhered to the distal esophagus for up to 3 hr in five of six patients with esophageal ulcers but adhered to only two of nine with lesser degrees of esophagitis. No adherence was seen in five patients without esophagitis. Thus, (/sup 99m/Tc)Su may not be useful for detecting any but the most severe grade of esophagitis. Based on these results, we speculate that the previously documented beneficial effects of sucralfate on mild to moderate esophagitis may be due to other mechanisms besides adherence to the ulcerated mucosa.

  13. Scintigraphic findings on {sup 99m}Tc-MDP, {sup 99m}Tc-sestamibi and {sup 99m}Tc-HMPAO images in Gaucher`s disease

    Energy Technology Data Exchange (ETDEWEB)

    Mariani, G. [Nuclear Medicine Service, DIMI, University of Genoa (Italy); Molea, N. [Regional Center of Nuclear Medicine, University of Pisa (Italy); La Civita, L. [Rheumatology Unit, University of Pisa (Italy); Porciello, G. [Rheumatology Unit, University of Pisa (Italy); Lazzeri, E. [Regional Center of Nuclear Medicine, University of Pisa (Italy); Ferri, C. [Rheumatology Unit, University of Pisa (Italy)

    1996-04-01

    We report here on the use of the lipophilic cationic complex technetium-99m sestamibi ({sup 99m}Tc-MIBI), employed as an indicator of increased cellular density and metabolic activity, to evaluate Gaucher cell infiltrates in the bone marrow; {sup 99m}Tc-hexametazime ({sup 99m}Tc-HMPAO) was also employed, as a pure indicator of lipidic infiltration in the bone marrow. A 67-year-old patient with known type 1 Gaucher`s disease presented with a painful left hip and knee and difficulty in gait subsequent to traumatic fracture of the left femoral neck that had required implant of a fixation screw-plaque. Bone scan with {sup 99m}Tc-methylene diphosphonate revealed reduced uptake at the distal metaphyseal-epiphyseal femoral region. In addition, whole-body maps and spot-view acquisitions of the thighs and legs were recorded at both 30 min and 2.5 h after the injection of {sup 99m}Tc-MIBI: the scintigraphic pattern clearly showed increased uptake at several sites involved by Gaucher deposits in the bone marrow (both knees, with variable intensity in different areas), matching the bone changes detected by conventional x-ray. The target to non-target ratios slowly decreased with time, from an average value of 2.25 in the early scan to an average value of 2 in the delayed scan. The lipid-soluble agent {sup 99m}Tc-HMPAO exhibited a superimposable scintigraphic pattern of accumulation at the involved sites, though with lower target to non-target ratios (1.27-1.48). The results obtained in this patient suggest a potential role of {sup 99m}Tc-MIBI in the scintigraphic evaluation of Gaucher`s lipid deposits in the bone marrow. If the results are confirmed in other patients, this radiopharmaceutical would offer clear advantages over {sup 133}Xe because of its wider availability and greater practicality (i.v. administration of {sup 99m}Tc-MIBI versus inhalation of {sup 133}Xe, and use of a single gamma camera instead of two as with {sup 133}Xe). (orig.). With 3 figs.

  14. A Comparative Study of 99mTc-YIGSR and 99mTc-MIBI Uptake in Tumor Cells

    Institute of Scientific and Technical Information of China (English)

    HU Jia; LAN Xiaoli; ZHANG Yongxue; GAO Zairong; WU Jun

    2005-01-01

    To investigate a new kind of tumor tracer 99mTc-YIGSR developed from a five amino structure (YIGSR) of the Laminin -chain,which can bind to the laminin receptors of tumor specifically, and radiolabeled with MAG3. (1) Preparation of the 99m Tc-YIGSR probe: with S-Acetly-NH3-MAG3as the chelator and with proper reductants YIGSR was labeled with 99mTc; (2) Cell culture and viability measurement: EAC was maintained in RPMI 1640 supplemented with calf serum; the trypan blue exclusion was applied to calculate the cell viability; (3) Study of the cell dynamic: The EACs uptake of 99mTc-YIGSR and99mTc-MIBI was observed at 37 ℃ and 22 ℃, respectively. (1)The labeling efficiencies of 99mTc-YIGSR and99mTc-MIBI were (62±3) % and (96±2) %, respectively; (2) The cell viability was declined with time of incubation; (3) At 37 ℃, the EACS uptake of 99mTc-YIGSR and99mTc-MIBI reached the peak of (43. 16±2.4) % and (24.4±1.8) % at 60min, respectively; and at 22 ℃, the highest uptake was (26.5±2.1) % and (9. 47±1.9) % at 60min, respectively. The in vitro study suggests that 99mTc-YIGSR is superior to 99mTc-MIBI in cell uptake and has potential value in tumor imaging.

  15. Biokinetics and dosimetry of {sup 99m} Tc-EDDA/HYNIC-[Lys{sup 3}]-bombesin in humans: imaging of GRP receptors

    Energy Technology Data Exchange (ETDEWEB)

    Santos C, C.L.; Ferro F, G. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico); Murphy, C.A de [INCMNSZ, 14000 Mexico D.F. (Mexico); Cardena, E.; Pichardo R, P. [Departamento de Medicina Nuclear, Oncologia Centro Medico Siglo XXI, Mexico D.F. (Mexico)

    2007-07-01

    Full text: Bombesin (BN) receptor subtype 2 (GRP-r) is over-expressed on various human tumors including breast, prostate, small cell lung and pancreatic cancer. Recently we reported the {sup 99-}mTc-EDDA/HYNIC-[Lys{sup 3}]-Bombesin ({sup 99m}Tc-HYNIC-BN) complex as a new radiopharmaceutical with high stability in human serum, specific cell GRP-receptor binding and rapid internalization. The aim of this study was to evaluate the {sup 99m}Tc-HYNIC-BN biokinetics and dosimetry in 5-healthy and 3-breast cancer women. Whole-body images were acquired at 20, 90, 180 min and 24 h after {sup 99m}Tc-HYNIC-BN administration. Regions of interest (ROIs) were drawn around source' organs on each time frame. The same set of ROIs was used for all 8 scans and the cpm of each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate {sup 99m}Tc-HYNIC-BN time activity curves in each organ, to calculate the total number of disintegrations (N) that occurred in the source regions. N data were the input for the OLINDA/EXM code to calculate internal radiation dose estimates. Images showed a rapid radiopharmaceutical blood clearance with predominantly renal excretion and minimal hepatobiliary elimination. {sup 99m}Tc-HYNIC-BN exhibited high in vivo affinity for GRP-r over-expression successfully visualized in breast cancer lesions and well differentiated from GRP-r expression in lungs and airways with normal GRP-r density (ratio 3:1). The equivalent doses for a study using 370 MBq were 7.38{+-}1.68, 0.59{+-}0.08, 2.07{+-}0.60, 0.58{+-}0.1, 0.75{+-}0.09 and 0.43{+-}0.07 mSv for kidneys, liver, lungs, ovaries, pancreas and red marrow respectively. The effective dose was 1.64{+-}0.25 mSv which is comparable with the doses known for most of the {sup 99m}Tc radiopharmaceutical studies in nuclear medicine. (Author)

  16. Evaluation of the absorbed dose to the kidneys due to Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3) and Tc{sup 99m} (Dmsa); Evaluacion de la dosis absorbida en los rinones debido al Tc{sup 99m} (DTPA) / Tc{sup 99m} (MAG3) y Tc{sup 99m} (DMSA)

    Energy Technology Data Exchange (ETDEWEB)

    Vasquez A, M.; Murillo C, F.; Castillo D, C.; Rocha J, J.; Sifuentes D, Y.; Sanchez S, P. [Universidad Nacional de Trujillo, Av. Juan Pablo II s/n, Trujillo (Peru); Idrogo C, J.; Marquez P, F., E-mail: marvva@hotmail.com [Instituto Nacional de Enfermedades Neoplasicas, Av. Angamos 2520, Lima (Peru)

    2015-10-15

    The absorbed dose in the kidneys of adult patients has been assessed using the biokinetics of radiopharmaceuticals containing Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3) or Tc{sup 99m} (Dmsa).The absorbed dose was calculated using the formalism MIRD and the Cristy-Eckerman representation for the kidneys. The absorbed dose to the kidneys due to Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3), are given by 0.00466 mGy.MBq{sup -1} / 0.00339 mGy.MBq{sup -1}. Approximately 21.2% of the absorbed dose is due to the bladder (content) and the remaining tissue, included in biokinetics of Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3). The absorbed dose to the kidneys due to Tc{sup 99m} (Dmsa) is 0.17881 mGy.MBq{sup -1}. Here, 1.7% of the absorbed dose is due to the bladder, spleen, liver and the remaining tissue, included in biokinetics of Tc{sup 99m} (Dmsa). (Author)

  17. {sup 99m}Tc-sestamibi. Clinical applications

    Energy Technology Data Exchange (ETDEWEB)

    Bucerius, Jan [Maastricht Univ. Medical Center (Netherlands). Dept. of Nuclear Medicine and Cardiovascular Research Institute Maastricht; Ahmadzadehfar, Hojjat; Biersack, Hans-Juergen (eds.) [Universitaetsklinikum Bonn (Germany). Klinik und Poliklinik fuer Nuklearmedizin

    2012-07-01

    {sup 99m}Tc-sestamibi is a single-photon emission computed tomography (SPECT) radiotracer that was introduced into clinical routine for myocardial perfusion imaging more than two decades ago. Although today the main application of {sup 99m}Tc-sestamibi SPECT remains the imaging of myocardial perfusion, it is also an accepted and well-proven imaging technique for a variety of oncologic and non-oncologic applications, including brain, breast, and thyroid cancer and thyroid and parathyroid adenoma. Its efficacy in a range of indications ensures that {sup 99m}Tc-sestamibi SPECT will remain widely used despite the rapid diffusion of 18F-FDG PET. {sup 99m}Tc-Sestamibi - Clinical Applications provides a detailed and informative overview of almost all the oncologic and non-oncologic applications of {sup 99m}Tc-sestamibi SPECT, including several relatively rare indications. Different disease-related protocols for {sup 99m}Tc-sestamibi SPECT are presented, and for each disease a comprehensive summary of the relevant pathology and epidemiology is provided. Throughout, there is a strong emphasis on the practical aspects of use of this popular tracer, including instructions for the preparation of several commercially available tracer kits. Clinical practitioners will find this book to be an invaluable guide to the application and benefits of {sup 99m}Tc-sestamibi SPECT in both the inpatient and the outpatient setting. (orig.)

  18. Tc-99m Ciprofloxacin SPECT of Pulmonary Tuberculosis

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Min Kyung; Hwang, Kyung Hoon [Gachon University Gil Hospital, Incheon (Korea, Republic of); Yoon, Min Ki [Good Samaritan Hospital, Pohang (Korea, Republic of); Choe, Won Sick [Kangbuk Samsung Hospital, Seoul (Korea, Republic of)

    2010-06-15

    Tc-99m ciprofloxacin is available for imaging infection. However, there has been no study on employing single photon emission computed tomography (SPECT) with using Tc-99m ciprofloxacin to image active pulmonary tuberculosis. Therefore, we conducted this study to assess the efficacy of Tc-99m ciprofloxacin SPECT for imaging active pulmonary tuberculosis. Twenty-one participants were enrolled in this prospective study. They were divided into two groups according to the clinical and radiological assessment. Group one (Gr. 1) consisted of five normal volunteers and six patients with inactive pulmonary tuberculosis. Group two (Gr. 2) consisted of ten patients with active pulmonary tuberculosis. SPECT was performed 3 h after injecting 555 MBq (15 mCi) of Tc-99m ciprofloxacin. The findings of Tc-99m ciprofloxacin SPECT were interpreted by a nuclear medicine specialist and then the results were analyzed according to the patients' clinical and radiological classifications. The results of Tc-99m ciprofloxacin SPECT were as follows: eight true-positive cases, ten true-negative cases, one false-positive case and two false-negative cases. The sensitivity and specificity was 80.0% and 90.0%, respectively. The positive predictive value was 88.9% and the negative predictive value was 83.3%. Conclusions Tc-99m ciprofloxacin SPECT is feasible for imaging active pulmonary tuberculosis. It is a useful nuclear-imaging method for discriminating between the active and inactive tuberculosis states in patients with a past medical history of pulmonary tuberculosis.

  19. Asialoglycoprotein receptor targeted imaging using Tc-99m galactosylated chitosan

    Energy Technology Data Exchange (ETDEWEB)

    Kim, E. M.; Jeong, H. J.; Kim, B. C.; Kim, C. K [Wonkang University College of Medicine, Iksan (Korea, Republic of)

    2004-07-01

    The asialoglycoprotein receptor (ASGP-R) is expressed on liver hepatocytes. Chitosan conjugates of galactose have shown to be specifically taken up by liver parenchymal cells via ASGP-R. In this study, Tc-99m hydrazino nicotinamide (HYNIC)-galactosylated chitosan (HYNIC-GC) was synthesized and evaluated as a targeted agent for the imaging of hepatocytes. GC was obtained after coupling of lactobionic acid as the galactose moiety and coupled with HYNIC. HYNIC-GC was radiolabeled with Tc-99m using stannous chloride and tricine as reducing agent and coligand respectively. Hepatic uptake property of Tc-99m HYNIC-GC was studied in female Balb/C mouse. Tc-99m HYNIC--GC and Tc-99m HYNIC-Chitosan as a control were intravenously injected into mice. Receptor binding was identified by coinjection with 50 mM and 80mM free galactose respectively. Biodistribution was determined at three different time points. The level of galactose substitution was 7.6%. Labeling efficiency was >90% both in vitro and serum up to 24 h. Tc-99m HYNIC-GC injected via tail vein of mice showed high selectivity of liver. On the other hands, Tc-99m HC without galactose group showed low uptake (Fig. 1A, 1B). Hepatic uptake of Tc-99m HYNIC-GC was dramatically blocked by 50 mM and 80 mM free galactose coinjection (Fig. 1C, 1D). The liver accumulated about 14 percent injected dose per gram (%ID/g) up to 120 min after injection. Tc-99m HYNIC-GC showed specific and rapid targeting to liver. It is a promising specific radiopharmaceutical with potential applications in the imaging of liver parenchymal cells.

  20. {sup 99m}Technetium labelled Escherichia coli

    Energy Technology Data Exchange (ETDEWEB)

    Diniz, S.O.F.; Cardoso, V.N. [Radioisotope Laboratory, Faculty of Pharmacy/UFMG, Belo Horizonte (Brazil); Resende, B.M.; Nunan, E.A. [Biological Control Laboratory, Faculty of Pharmacy/UFMG, Belo Horizonte (Brazil); Simal, C.J.R. [Laboratory Nuclear Medicine, Faculty of Medicine/UFMG, Belo Horizonte (Brazil)

    1999-07-01

    Samples of a culture of unlabeled Escherichia coli were incubated with different concentrations of stannous chloride for various time periods. {sup 99m}Tc (26.0 MBq) was added to each preparation and the results showed a labelling yield of 98% for E. coli. Since the bacterial viability of {sup 99m}Tc-E. coli and E. coli did not show any statistical differences, these results demonstrate that labelling of E. coli with {sup 99m}Tc does not modify the bacterial viability, and the radiolabelled bacteria may be a good model to study bacterial translocation.

  1. Effects of concurrent drug therapy on technetium /sup 99m/Tc gluceptate biodistribution

    Energy Technology Data Exchange (ETDEWEB)

    Hinkle, G.H.; Basmadjian, G.P.; Peek, C.; Barker, K.K.; Ice, R.D.

    1982-11-01

    Drug interactions with /sup 99m/Tc gluceptate resulting in altered biodistribution were studied using chart review and animal tests. Charts of nine patients who had abnormal gallbladder uptake of technetium /sup 99m/Tc gluceptate during a two-year period were reviewed to obtain data such as concurrent drug therapy, primary diagnosis, and laboratory values. Adult New Zealand white rabbits were then used for testing the biodistribution of technetium /sup 99m/Tc gluceptate when administered concurrently with possibly interacting drugs identified in the chart review--penicillamine, penicillin G potassium, penicillin V potassium, acetaminophen, and trimethoprim-sulfamethoxazole. Chart review revealed no conclusive patterns of altered biodistribution associated with other factors. The data did suggest the possibility that the five drugs listed above might cause increased hepatobiliary clearance of the radiopharmaceutical. Animal tests showed that i.v. penicillamine caused substantial distribution of radioactivity into the gallbladder and small bowel. Minimally increased gallbladder radioactivity occurred when oral acetaminophen and trimethoprim-sulfamethoxazole were administered concurrently. Oral and i.v. penicillins did not increase gallbladder activity. Penicillamine may cause substantial alteration of the biodistribution of technetium /sup 99m/Tc gluceptate.

  2. Exploring the Potential of (99m)Tc(CO)3-Labeled Triazolyl Peptides for Tumor Diagnosis.

    Science.gov (United States)

    Gaonkar, Raghuvir H; Ganguly, Soumya; Baishya, Rinku; Dewanjee, Saikat; Sinha, Samarendu; Gupta, Amit; Ganguly, Shantanu; Debnath, Mita C

    2016-04-01

    In recent years the authors have reported on (99m)Tc(CO)3-labeled peptides that serve as carriers for biomolecules or radiopharmaceuticals to the tumors. In continuation of that work they report the synthesis of a pentapeptide (Met-Phe-Phe-Gly-His; pep-1), a hexapeptide (Met-Phe-Phe-Asp-Gly-His; pep-2), and a tetrapeptide (Asp-Gly-Arg-His; pep-3) and the attachment of 3-amino-1,2,4-triazole to the β carboxylic function of the aspartic acid unit of pep-2 and pep-3. The pharmacophores were radiolabeled in high yields with [(99m)Tc(CO)3(H2O)3](+) metal aqua ion, characterized for their stability in serum and saline, as well as in His solution, and found to be substantially stable. B16F10 cell line binding studies showed favorable uptake and internalization. In vivo behavior of the radiolabeled triazolyl peptides was assessed in mice bearing induced tumor. The (99m)Tc(CO)3-triazolyl pep-3 demonstrated rapid urinary clearance and comparatively better tumor uptake. Imaging studies showed visualization of the tumor using (99m)Tc(CO)3-triazolyl pep-3, but due to high abdominal background, low delineation occurred. Based on the results further experiments will be carried out for targeting tumor with triazolyl peptides.

  3. Discordant results in Tc-99m tetrofosmin and Tc-99m sestamibi parathyroid scintigraphies; Resultados discordantes em cintilografias das paratireoides realizadas com tetrofosmin-99mTc e com sestamibi-99mTc

    Energy Technology Data Exchange (ETDEWEB)

    Duarte, Paulo Schiavom; Domingues, Fernanda C.; Santi Costa, Michele; Brandao, Cynthia; Oliveira, Marco A.C. de; Vieira, Jose G.H. [Fleury - Centro de Medicina Diagnostica, Sao Paulo, SP (Brazil)]. E-mail: paulo.duarte@fleury.com.br

    2007-10-15

    Parathyroid scintigraphies have been used to detect pathological parathyroid glands either before as well as after the parathyroid resection surgery in patients with hyperparathyroidism. One of the most utilized techniques to perform the studies is the double-phase images with Tc-99m sestamibi, which has been shown to be very accurate in the localization of enlarged parathyroid glands. Similar to Tc-99m sestamibi, Tc-99m tetrofosmin is a radiopharmaceutical initially developed to perform myocardial perfusion study that has been used to perform parathyroid scintigraphies. Although most of the papers suggest that the overall sensitivities of both radiopharmaceuticals are similar, there are some papers questioning the accuracy of Tc-99m tetrofosmin to detect abnormal parathyroid glands. In the present article, we report a case with discordant results by both methods. (author)

  4. Monoclonal anti-elastin antibody labelled with technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Marcia B.N. de; Silva, Claudia R. da; Araujo, Adriano C. de; Bernardo Filho, Mario [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Instituto de Biologia Roberto Alcantara Gomes. Lab. de Radiofarmacia; Porto, Luis Cristovao M.S.; Gutfilen, Bianca [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Instituto de Biologia Roberto Alcantara Gomes; Souza, J.E.Q. [Instituto Nacional do Cancer, Rio de Janeiro, RJ (Brazil). Centro de Pesquisa Basica; Frier, Malcolm [University Hospital, Nottingham (United Kingdom). Dept. of Medical Physics

    1999-11-01

    Technetium-99m ({sup 99m} Tc) is widely employed in nuclear medicine due to its desirable physical, chemical and biological properties. Moreover, it is easily available and normally is inexpensive. A reducing agent is necessary to label cells and molecules with {sup 99m} Tc and stannous chloride (Sn C L{sub 2}) is usually employed. Elastin is the functional protein component of the elastic fiber and it is related with some diseases such as arteriosclerosis, pulmonary emphysema and others. The present study refers to the preparation of the {sup 99m} Tc labeled monoclonal anti-elastin antibody. The monoclonal antibody was incubated with an excess of 2-iminothiolane. The free thiol groups created, were capable of binding with the reduced technetium. Labeling was an exchange reaction with {sup 99m} Tc-glucoheptonate. The labeled preparation was left at 4 deg C for one hour. Then, it was passed through a Sephadex G50 column. Various fractions were collected and counted. A peak corresponding to the radiolabeled antibody was obtained. Stability studies of the labelled anti-elastin were performed at 0,3 6, 24 hours, at both 4 deg C or room temperature. The biodistribution pattern of the {sup 99m} Tc-anti-elastin was studied in healthy male Swiss mice. The immunoreactivity was also determined. An useful labeled-anti-elastin was obtained to future immunoscintigraphic investigations. (author) 4 refs., 7 figs., 6 tabs.

  5. Development of superior bone scintigraphic agent from a series of {sup 99m}Tc-labeled zoledronic acid derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Lin Jianguo [Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063 (China); Qiu Ling, E-mail: qiulingwx@gmail.com [Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063 (China); Cheng Wen [Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063 (China); Luo Shineng, E-mail: shineng914@yahoo.com.cn [Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063 (China); Xue Li; Zhang Shu [Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063 (China)

    2012-05-15

    Two novel zoledronic acid (ZL) derivatives, 1-hydroxy-4-(1H-imidazol-1-yl)butane-1,1-diyldiphosphonic acid (IBDP) and 1-hydroxy-5-(1H-imidazol-1-yl)pentane-1,1-diyldiphosphonic acid (IPeDP), were prepared and labeled with the radionuclide technetium-99m in a high labeling yield. In vitro stabilities of these radiolabeled complexes were measured by the radio-HPLC analysis as a function of time, which showed excellent stability with the radiochemical purity of over 95% at 6 h post preparation. Their in vivo biological performances were evaluated and compared with those of {sup 99m}Tc-ZL and {sup 99m}Tc-MDP (methylenediphosphonic acid). The biodistribution in mice and scintigraphic images of the rabbit showed that the tracer agent {sup 99m}Tc-IPeDP had highly selective uptake in the skeletal system and rapid clearance from the blood and soft tissues and an excellent scintigraphic image can be obtained in a shorter time post injection with clear visualization of the skeleton and low soft tissue activity. These preclinical studies suggest that {sup 99m}Tc-IPeDP would be a novel superior bone scintigraphic agent. - Highlights: Black-Right-Pointing-Pointer Two novel diphosphonic acids were labeled with the {sup 99m}Tc in high labeling yield. Black-Right-Pointing-Pointer {sup 99m}Tc-IPeDP had high uptake in skeletal system and rapid clearance from blood. Black-Right-Pointing-Pointer {sup 99m}Tc-IPeDP reveals attractive biological features as superior bone scanning agent.

  6. Evaluation of {sup 99m}Tc-glucarate as a breast cancer imaging agent in a xenograft animal model

    Energy Technology Data Exchange (ETDEWEB)

    Gambini, Juan Pablo [Nuclear Medicine Center, Clinical Hospital, University of Uruguay, Montevideo, 11600 (Uruguay); Cabral, Pablo [Nuclear Investigations Center, School of Science, University of Uruguay, Montevideo, 11400 (Uruguay); Alonso, Omar [Nuclear Medicine Center, Clinical Hospital, University of Uruguay, Montevideo, 11600 (Uruguay); Savio, Eduardo [Department of Radiochemistry, School of Chemistry, University of Uruguay, Montevideo, 11800 (Uruguay); Daibes Figueroa, Said [Research Service, Harry S. Truman Veterans Memorial Hospital, Columbia, MO 65201 (United States); Zhang Xiuli [Research Service, Harry S. Truman Veterans Memorial Hospital, Columbia, MO 65201 (United States); Department of Biochemistry, University of Missouri, Columbia, MO 65211 (United States); Ma Lixin [Research Service, Harry S. Truman Veterans Memorial Hospital, Columbia, MO 65201 (United States); Department of Radiology, University of Missouri, Columbia, MO 65212 (United States); Deutscher, Susan L. [Research Service, Harry S. Truman Veterans Memorial Hospital, Columbia, MO 65201 (United States); Department of Biochemistry, University of Missouri, Columbia, MO 65211 (United States); Quinn, Thomas P., E-mail: quinnt@missouri.ed [Research Service, Harry S. Truman Veterans Memorial Hospital, Columbia, MO 65201 (United States); Department of Biochemistry, University of Missouri, Columbia, MO 65211 (United States); Department of Radiology, University of Missouri, Columbia, MO 65212 (United States)

    2011-02-15

    Introduction: The use of [{sup 99m}Tc]glucarate has been reported as an infarct-avid agent with the potential for very early detection of myocardial infarction. [{sup 99m}Tc]Glucarate has also been postulated as an agent for non-invasive detection of tumors. The aim of our study was to develop a Glucarate kit and evaluate [{sup 99m}Tc]glucarate as a potential cancer imaging agent in female SCID mice bearing human MDA-MB-435 breast tumors. Methods: Glucarate in a kit formulation was labeled with {sup 99m}Tc and evaluated for radiolabelling efficiency and radiochemical purity. The Glucarate kit stability was assessed by monthly quality controls. The pharmacokinetics of [{sup 99m}Tc]glucarate were determined in female SCID mice bearing MDA-MB-435 human breast carcinoma tumors at 0.5, 1, 2, 4 and 24 h. Nuclear imaging studies were performed with a micro-single photon emission tomography (SPECT)/computed tomography (CT) system at 2 h post injection, while magnetic resonance imaging (MRI) was employed for tumor morphology analysis and metastatic deposit localization. Results: The Glucarate kits exhibited a stable shelf life of 6 months. [{sup 99m}Tc]Glucarate was obtained with radiochemical purity greater than 95%. Biodistribution studies demonstrated moderate tumor uptake coupled with high renal clearance. Tumor-to-muscle ratios were 4.85 and 5.14 at 1 and 4 h post injection. MRI analysis showed tumors with dense cellular growth and moderate central necrosis. [{sup 99m}Tc]Glucarate uptake in the primary MDA-MB-435 shoulder tumors and metastatic lesions were clearly visualized with micro-SPECT/CT imaging. Conclusions: Selective tumor uptake and rapid clearance from nontarget organs makes [{sup 99m}Tc]glucarate a potential agent for breast cancer imaging that awaits validation in a clinical trial.

  7. Poor Tc-99m dimercaptosuccinic acid uptake, re-evaluation with Tc-99m MAG3 scintigraphy in Lowe syndrome

    OpenAIRE

    Koca, Gokhan; Atilgan, Hasan Ikbal; Demirel, Koray; Diri, Akif; KORKMAZ, Meliha

    2011-01-01

    Tc-99m dimercaptosuccinic acid (DMSA) is filtered through the glomeruli and reabsorbed by the proximal tubules as low molecular weight proteins. In Lowe syndrome this mechanism is impaired and so poor DMSA uptake is seen. Poor DMSA uptake was shown in very few studies, but none mentioned normal Tc-99m MAG3 uptake. In this case, the patient had poor DMSA uptake, normal MAG3 uptake and a neurogenic bladder in anterior to the left kidney that attenuates left kidney.

  8. Fab(nimotuzumab)-HYNIC-99mTc: Antibody Fragmentation for Molecular Imaging Agents.

    Science.gov (United States)

    Calzada, Victoria; García, María Fernanda; Alonso-Martínez, Luis Michel; Camachoc, Ximena; Goicochea, Enzo; Fernández, Marcelo; Castillo, Abmel Xiques; Díaz-Miqueli, Arlhee; Iznaga-Escobar, Normando; Montaña, René Leyva; Alonso, Omar; Gambini, Juan Pablo; Cabral, Pablo

    2016-01-01

    Finally, fast blood clearance nimotuzumab is a humanized monoclonal antibody that recognise, with high specific affinity, the epidermal growth factor receptor (EGF-R) which play an important role in the growth process associated with many solid tumors. In this work, the whole antibody was digested with papain in order to generate a Fab fragment, derivatized with NHS-HYNIC-Tfa and radiolabel with technetium-99m (99mTc) as a potential agent of molecular imaging of cancer. Both, whole and fragment radiolabels were in-vivo and in-vitro characterized. Radiolabeling conditions with Tricine as coligand and quality controls were assessed to confirm the integrity of the labeled fragment. Biodistribution and imaging studies in normal and spontaneous adenocarcinoma mice were performed at different times to determine the in-vivo characteristics of the radiolabel fragment. Tumor localization was visualized by conventional gamma camera imaging studies, and the results were compared with the whole antibody. Also, an immunoreactivity assay was carried out for both. The results showed clearly the integrity of the nimotuzumab fragment and the affinity by the receptor was verified. Fab(nimotuzumab)-HYNIC was obtained with high purity and a simple strategy of radiolabeling was performed. Finally, a fast blood clearance was observed in the biodistribution studies increasing the tumor uptake of Fab(nimotuzumab)- HYNIC-99mTc over time, with tumor/muscle ratios of 3.81 ± 0.50, 5.16 ± 1.97 and 6.32 ± 1.98 at 1 h, 4 h and 24 h post injection. Urinary excretion resulted in 32.89 ± 3.91 %ID eliminated at 24 h. Scintigraphy images showed uptake in the tumor and the activity in non-target organs was consistent with the biodistribution data at the same time points. Hence, these preliminary results showed important further characteristic of Fab(nimotuzumab)-HYNIC-99mTc as a molecular imaging agent of cancer.

  9. {sup 99m}Tc-ECD and {sup 99m}Tc-HM-PAO SPECT in five patients with MELAS

    Energy Technology Data Exchange (ETDEWEB)

    Katagiri, Shinako; Nishimaki, Hiroshi; Kitano, Masashi; Horiike, Shigeharu; Kan, Shinichi; Ishii, Katsumi; Matsubayashi, Takashi; Sakai, Fumihiko [Kitasato Univ., Sagamihara, Kanagawa (Japan). School of Medicine

    1998-03-01

    Cerebral perfusion was studied in five patients with mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS), using single photon emission computed tomography (SPECT) with {sup 99m}Tc-ethyl cysteinate dimer ({sup 99m}Tc-ECD) or {sup 99m}Tc-hexamethyl propyleneamine oxime ({sup 99m}Tc-HM-PAO). In four cases, regional cerebral blood flow (rCBF) was evaluated by the method reported by Mastuda et al. Immediately after the stroke-like episodes, accumulation of the tracer was relatively increased in the temporooccipital lobe, and also increased rCBF was shown in the same area. However, the region showed decreased radioactivity at the chronic stage, and rCBF decreased also. These findings are consistent with positron emission tomography (PET) at the acute stage and autopsy. {sup 99m}Tc-ECD SPECT and {sup 99m}Tc-HM-PAO SPECT may be useful in the diagnosis and assessment of the progress of the MELAS. (author)

  10. Rapid diagnosis of occult abscesses using sup 99m Tc-labeled monoclonal antibodies

    Energy Technology Data Exchange (ETDEWEB)

    Coons, T.A.; Rhodes, B.A. (RhoMed, Inc., Albuquerque, NM (USA)); Thakur, M.L. (Thomas Jefferson Univ., Philadelphia, PA (USA)); Marcus, C.S. (Harbor-UCLA Medical Center, Torrance, CA (USA)); Ballou, B. (Pittsburgh Univ., PA (USA))

    1991-01-01

    Acute infections, such as appendicitis and occult infections in AIDS patients, can be diagnosed within two hours by gamma scintigraphy after i.v. administration of {sup 99m}Tc labeled antibodies reactive with human granulocytes. The antibody, murine IgM anti-SSEA-1, is partially reduced using Sn(II) to expose and protect reactive sulfide groups. The antibody is then purified, stannous tartrate and stabilizers are added, and the mixture is lyophilized. To label, sodium pertechnetate is added. After a 15 minute incubation the tracer drug is injected. The rate of accumulation and degree of concentration at the site of infection is presumptively determinative of the severity of the infection. Acceptance criteria and tests for the {sup 99m}Tc labeled antibody product have been established and validated. Greater than 93% of the {sup 99m}Tc is firmly bound to the protein as determined by quantitative HPLC. Radiochemical impurities, colloidal {sup 99m}Tc and free pertechnetate are together less than 4% as determined by thin layer chromatography. The immunoreactive fraction, measured by binding to solid phase antigen, and affinity measured be ELISA, are unchanged by the {sup 99m}Tc-direct labeling process. Two hour blood clearance in rats is within 90% of the value of the {sup 125}I labeled analog. The immunoreactive fraction decreases less than 10% when incubated in human plasma for 24 hours. This method has been compared to other direct labeling methods, and found to give higher radiochemical yields. 5 figs.

  11. (99m)Tc-zolmitriptan: radiolabeling, molecular modeling, biodistribution and gamma scintigraphy as a hopeful radiopharmaceutical for lung nuclear imaging.

    Science.gov (United States)

    Rashed, H M; Marzook, F A; Farag, H

    2016-12-01

    Lung imaging radiopharmaceuticals are helpful agents for measuring pulmonary blood flow and allow detection of pulmonary embolism and lung cancer. The goal of this study was to develop a novel potential radiopharmaceutical for lung imaging. Zolmitriptan (a selective serotonin receptor agonist) was successfully labeled with (99m)Tc via direct labeling method under reductive conditions studying different factors affecting the labeling efficiency. (99m)Tc-zolmitriptan was obtained with a maximum labeling yield of 92.5 ± 0.61 % and in vitro stability up to 24 h. Molecular modeling was done to predict the structure of (99m)Tc-zolmitriptan and ensure that radiolabeling did not affect binding ability of zolmitriptan to its receptor. Biodistribution studies showed that maximum lung uptake of (99m)Tc-zolmitriptan was 23.89 ± 1.2 % injected dose/g tissue at 15 min post-injection and retention in lungs remained high up to 1 h, whereas the clearance from mice appeared to proceed mainly via the renal pathway. Scintigraphic images confirmed the biodistribution results showing a high resolution lung image with low accumulation of radioactivity in other organs except kidneys and urinary bladder. (99m)Tc-zolmitriptan is not a blood product and so it is more safe than the currently available (99m)Tc-MAA, and its lung uptake is higher than that of the recently discovered (123)I-IPMPD, (99m)Tc(CO)5I and (99m)Tc-DHPM. So, (99m)Tc-zolmitriptan could be used as a hopeful radiopharmaceutical for lung scintigraphic imaging.

  12. Tc-99m-tetrofosmin scintigraphy: an alternative scintigraphic method for following up differentiated thyroid carcinoma--preliminary results.

    Science.gov (United States)

    Gallowitsch, H J; Kresnik, E; Mikosch, P; Pipam, W; Gomez, I; Lind, P

    1996-12-01

    The usefulness of the myocardial perfusion agent, Tc-99m-tetrofosmin (Myoview) in the follow-up of differentiated thyroid carcinoma was evaluated in a clinical study of 15 patients, primary treated with thyroidectomy and high-dose I-131-therapy (2960-3700 MBq). 12 with suspected recurrence and metastases and three patients without any suspicion and compared with other non-specific tracers like TI-201 and Tc-99m-sestamibi. Twelve patients with elevated thyroglobulin (Tg) levels of more than 10 ng/ml (group A), four of these had negative I-131 scans, and three patients with Tg levels less than 10 ng/ml (group B) were examined under TSH suppressive L-Thyroxine treatment. Whole body scans were taken with Tl-201 (74 MBq: 20 mn post injection), Tc-99m-sestamibi (370 MBq: 20-60 min post injection) and Tc-99m-tetrofosmin (370 MBq: 20-60 min post injection). Tumor/background ratios and optional time/activity analyses (up to 150 min post injection) were evaluated using the region of interest approach. Compared with Tl-201 (T/BG: 1.59, +/- 0.396). Tc-99m-tetrofosmin showed slightly but not significant better T/BG ratios and detection rates (T/BG: 1.76, +/- 0.345). Tc-99m-sestamibi (1.51, +/- 0.31 p = 0.05) showed significantly lower values than Tc-99m-tetrofosmin In the light of these results, scintigraphy with Tc-99m-tetrofosmin seems to be a possibly sensitive imaging modality in the follow-up of DTC with possible advantages concerning T/Bg ratio, background clearance, detection rate and dosimetry compared with Tl-201 and Tc-99m-sestamibi, especially in patients with elevated Tg level and no iodine uptake, but further investigations are needed to confirm our preliminary results.

  13. Perfusion lymphoscintigraphy using sup 99m Tc-human serum albumin in patients with treated uterine cancer

    Energy Technology Data Exchange (ETDEWEB)

    Kataoka, Masaaki; Kawamura, Masashi; Nishiyama, Yasuyuki; Itoh, Hisao; Hamamoto, Ken; Hamada, Katsuyuki; Matsuura, Shumpei (Ehime Univ., Shigenobu (Japan). School of Medicine)

    1991-07-01

    Perfusion lymphoscintigraphy was performed by subcutaneous injection of 7.4 MBq (0.2 mCi) {sup 99m}Tc-human serum albumin ({sup 99m}Tc-HSA) in 25 patients with uterine cancer treated by operation and/or irradiation. Radioactivity at the injection site was counted for 3 min at 10 min (a) and at 3 hr (b) after injection, and the clearance of {sup 99m}Tc-HSA was defined as (1-(b)/(a))x100(%) ((a) and (b) were corrected for decay of the isotope). The clearance in legs with lymphedema was significantly more delayed than those in legs without lymphedema in patients treated with both operation and irradiation (16.6{+-}7.7% vs 34.9{+-}9.3%; p<0.01) and in patients treated with radiation therapy alone (33.1{+-}7.4 vs 48.0{+-}5.6; p<0.01). The clearances in edematous legs in a case which had developed venous occulusion after operation were 44.2% and 41.7%, which were almost the same as those in the non-edematous patients treated with operation alone. Clearance in patients treated with both operation and irradiation were significantly more delayed than those in patients treated with a single modality of operation or irradiation (30.1{+-}11.4% vs 41.9{+-}8.9%, 42.0{+-}9.7%, respectively; p<0.01). Radiation doses at points B were well correlated with clearance of {sup 99m}Tc-HSA (p<0.05). These data suggest that perfusion lymphoscintigraphy using {sup 99m}Tc-HSA is useful for evaluating the patients with lymphedema and for differentiating it from the edema caused by the other mechanisms. It is also suggested that radiation dose is one of factors in the occurrence of lymphedema. (author).

  14. Technetium-99m nitrido radiopharmaceuticals with unprecedented biological properties

    Directory of Open Access Journals (Sweden)

    Adriano Duatti

    2002-09-01

    Full Text Available The chemical methods for the production of technetium-99m radiopharmaceuticals containing a terminal TcºN triple bond have been established more than a decade ago. From that time, the chemistry of nitrido Tc-99m complexes has provided a highly efficient tool for the design and preparation of novel classes of diagnostic agents, and a number of potentially useful radiopharmaceuticals have been discovered. In particular, nitrido technetium-99m tracers have been developed for heart perfusion imaging. In this short review, the chemical and biological properties of the neutral myocardial perfusion tracer bis(N-ethoxy, N-ethyl-dithiocarbamato nitrido Tc-99m (TcN-NOEt will be summarized along with the preparation and preliminary biological evaluation of the first class of monocationic nitrido technetium-99m radiopharmaceuticals exhibiting improved biodistribution properties closer to those expected for an ideal perfusion imaging agent.Os métodos químicos para produção de radiofármacos marcados com tecnécio-99m contendo a ligação tripla terminal TcºN foram estabelecidos há mais de uma década. Desde esta época, a química dos complexos nitridos marcados com 99mTc tem sido uma ferramenta altamente eficiente para o desenho e preparo de novas classes de agentes para diagnóstico e, foi descoberto um número de radiofarmacos potencialmente úteis. Nesta pequena revisão, as propriedades biológicas e químicas do traçador para perfusão miocárdica neutra, o bis(N-etoxi, N-etil-ditiocarbamato nitrido 99mTc (TcN-NOEt, serão resumidas junto com o preparo e avaliação biológica preliminar da primeira classe de radiofármacos nitrido monocatiônico marcado com tecnécio-99m que exibe melhores propriedades em relação à biodistribuição, mais próximas daquelas esperadas para um agente perfusor ideal para imagens.

  15. Labeling cellular elements of blood with Technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Dewanjee, M.K.

    1990-08-01

    The purpose of this proposal is to develop new technique of labeling platelets and white cells with Tc-99m radionuclide. The conditions of labeling canine platelets and white cells with the lipid-soluble Tc-99m HMPAO have been optimized. The function of labeled platelets were evaluated by the determination of platelet survival time and recovery and these values were compared with that of In-111 tropolone labeled platelets. We developed the bilateral femoral catheterization model for the evaluation of platelet-thrombosis on control and heparin-bonded catheters in dogs. We are evaluating platelet thrombosis in the hollow-fiber hemodialyzer with Tc-99m and In-111 labeled platelets. We have developed the flow-loop for in vitro studies and are using a pig model for quantitation of platelet-consumption during hemodialysis. We are currently evaluating the new technique of platelet and white cell-labeling with Tc-99m and testing them in animal models of thrombosis and infection (osteo-myelitis). We are also using the Tc-99m HMPAO labeled mixed white cells in the early diagnosis (3-hour post-injection) of acute and chronic infection in patients and comparing the results with that of IN-111 oxine labeled white cells.

  16. Technique using Technetium-99m-MIBI Uptake Kinetics

    Directory of Open Access Journals (Sweden)

    Shinro Matsuo

    2013-04-01

    Full Text Available Mitochondria play an important role in energy production for the cell. The proper function of a myocardial cell largely depends on the functional capacity of the mitochondria.  Therefore it is necessary to establish a novel and reliable method for a non-invasive assessment of mitochondrial function and metabolism in humans. Although originally designed for evaluating myocardial perfusion, 99mTc-MIBI can be also used to evaluate cardiac mitochondrial function. In a clinical study on ischemic heart disease, reverse redistribution of 99mTc-MIBI was evident after direct percutaneous transluminal coronary angioplasty. The presence of increased washout of 99mTc-MIBI was associated with the infarct-related artery and preserved left ventricular function. In non-ischemic cardiomyopathy, an increased washout rate of 99mTc-MIBI, which correlated inversely with left ventricular ejection fraction, was observed in patients with congestive heart failure. Increased 99mTc-MIBI washout was also observed in mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS and in doxorubicin-induced cardiomyopathy. Noninvasive assessment of cardiac mitochondrial function could be greatly beneficial in monitoring possible cardiotoxic drug use and in the evaluation of cardiac damage in clinical medicine.

  17. Synthesis, radiolabeling and in vivo biological evaluation of {sup 99m}Tc-labeled MAG{sub 3}-based bisnitroimidazole complexes as tumor hypoxia markers

    Energy Technology Data Exchange (ETDEWEB)

    Mei, Lei; Chu, Taiwei [Peking Univ., Beijing (China). Beijing National Laboratory for Molecular Sciences, Radiochemistry and Radiation Chemistry

    2014-04-01

    Hypoxia, as a common phenomenon in solid tumors, is of interest for its relationship with resistance to tumor therapies and malignant progression of tumor. The noninvasive nuclear medical imaging technique using hypoxia markers is an important method for the detection of tumor hypoxia. The aim of current study is designing tumor hypoxia markers with hypoxia selectivity and improved properties. Two MAG{sub 3}-based bisnitroimidazole compounds were synthesized and purified by semi-preparative HPLC. Both the MAG{sub 3} derivatives were labeled with {sup 99m}Tc-oxo-technetium core via stannous tartrate exchange method in high yields (> 95%). The {sup 99m}Tc-MAG{sub 3} complexes were stable at 37 C, 4 h after preparation, and were more hydrophilic than {sup 99m}Tc-MAMA complexes. As biodistribution results showed, clearances of background activity for both the complexes were fast and they were excreted mainly through the hepatobiliary tract and part of renal tract. Although tumor uptakes of {sup 99m}Tc-MAG{sub 3}-B2NIL were lower than those of {sup 99m}Tc-MAG{sub 3}-B4NIL, tumor-to-blood ratios of {sup 99m}Tc-MAG{sub 3}-B2NIL showed an increasing trend and were better than those of {sup 99m}Tc-MAG{sub 3}-B4NIL after 2 h due to their different blood clearances. Tumor-to-muscle ratios of {sup 99m}Tc-MAG{sub 3}-B2NIL and {sup 99m}Tc-MAG{sub 3}-B4NIL were similar. Comparing with {sup 99m}Tc-MAMA complexes, {sup 99m}Tc-MAG{sub 3}-B2NIL with better tumor-to-blood ratios exhibits improved feature for hypoxia imaging, though it has lower tumor uptake than {sup 99m}Tc-MAMA complexes. (orig.)

  18. Technetium 99m stannous glucoheptonate for renal imaging and evaluating individual renal function

    Energy Technology Data Exchange (ETDEWEB)

    Corriere, J.N. Jr.; Staum, M.M.; Gadbois, W.F.; Benson, G.S.

    1975-01-01

    Technetium 99m stannous glucoheptonate is a new radiopharmaceutical that, on intravenous administration, is said to be useful for kidney imaging and limited flow studies. The kidney-localizing properties of the agent may be due to binding in the proximal and distal tubule cells, and as such reflect functioning tubular mass. This mechanism is similar to that of chlormerodrin Hg 197 but delivers only 0.06% of the radiation of the mercurial compound. To test the validity of these expectations, we compared the actions of this compound to the images and functional data of 48-hr delayed chlormerodrin scans as well as conventional clearances and excretory urograms in the canine.

  19. Gastric Reflux on Routine Tc-99m DISIDA hepatobiliary Scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Kang Wook; Lee, Heon Young [Chungnam National University College of Medicine, Daejeon (Korea, Republic of)

    1995-06-15

    Reflux of bile and digestive enzymes from the small bowel and duodenum into stomach has been observed in patients with various gastroduodenal diseases. Tc-99m iminodiacetic acid derivatives hepatobiliary scan has been used as a noninvasive method to detect duodenogastric reflux. Sometimes, gastric reflux can be observed incidentally on routine Tc-99m DISA hepatobiliary scintigraphy. To evaluate the clinical meaning of gastric reflux on routine Tc-99m DISIDA hepatobiliary scan, we analyzed 36 patients showed gastric reflux incidentally on the routine Tc-99m-DISIDA hepatobiliary scintigraphy from December 1991 to June 1995 in Chungnam National University Hospital. The results were as follows: 1) The gastric reflux was observed in 2.3% of 1,553 cases of routine Tc-99m DISIDA Hepatobiliary scintigraphy for 43 months. 2) Nineteen percent of patients with gastric reflux had the past medical history of operations on stomach or biliary system. And that history was more prevalent in patients with reflux than those without reflux, significantly (p<0.01). 3) On fiberoptic gastroduodenpscopic examination, 87% of the patients with gastric reflux had the gastroduodenal diseases such as gastritis, gastric ulcer, duodenal ulcer, gastric cancer, duodenal cancer and ampullary diverticulosis. We thought that the gastric reflux can be observed considerably in patients without any operation history on stomach or duodenum, although the operation history is more prevalent in patients with gastric reflux than those without reflux, significantly and most of patients with gastric reflux on routine Tc-99m DISIDA scan has various gastroduodenal diseases.

  20. Technetium-99m radiopharmaceuticals for in vivo diagnostics

    Directory of Open Access Journals (Sweden)

    Đokić Divna Đ.

    2005-01-01

    Full Text Available Technetiiim-99m is an ideal radionuclide with optimum decay characteristics. It can be obtained in sterile, pyrogen-free and carrier-free condition, as sodium pertechnetate (Na99mTcO4, from 99Mo/99Tc generator. Because of its six-hour physical half-life and monochromatic 140 keV photons free of -radiation, administration of small amounts of 99mTc solution is possible, without a significant radiation damage to the patient. Technetium eluted from the 99Mo/99mTc generator is in the highest oxidation form (+7. It can be used for diagnostic purposes alone, but it is often used for labeling different organic and inorganic compounds. As it is unreactive, reduction with a chemical reductant, (+1, (+3 and (+5 oxidation are necessary before use. Nowadays almost 80% of radiopharmaceuticals are based on 99mTc. Radiopharmaceuticals. Radiopharmaceuticals are radionuclides or radioactive compounds used in diagnosis and therapy of human diseases. A pharmaceutical is chosen based on its localization in the organ, or its participation in its physiological function. Radiation emitted from a radionuclide is detected by a radiation detector. The ability to incorporate available radionuclides into tracer molecules has been the main goal in developing radiopharmaceuticals. As radionuclides with nuclear characteristics used as either diagnostic or therapeutic radiopharmaceuticals, are predominantly metals, they can be designed as metal essential, whereby biological distribution is determined by coordination compound, or metal tagged, in which case the properties of the carrier molecule (ligand system determine the biological distribution. This paper reviews the development of 99mTc-radiopharmaceuticals. .

  1. Novel Tc-99m radiotracers for brain imaging

    Directory of Open Access Journals (Sweden)

    Alessandra Boschi

    2007-09-01

    Full Text Available A novel class of Tc-99m complexes able to cross the blood brain barrier has been investigated and described here. These compounds are formed by reacting the bis-substituted nitrido precusors [99mTc(N(PS2] (PS = phosphino-thiol ligand with triethylborane (BEt3 under strictly anhydrous conditions and using non-aqueous solvents. The molecular structure of these tracers was not fully established, but preliminary experimental evidence suggests that they result from the interaction of the Lewis base [99mTc(N(PS2] with the Lewis acid BEt3, which leads to the formation of the novel -B-Nº Tc- adduct. After purification and recovery in a physiological solution, the new borane-nitrido Tc-99m derivatives were injected in rats for evaluating their in vivo biological behavior. Results showed a significant accumulation in brain tissue, thus indicating that these complexes are capable of penetrating the intact blood brain barrier. Uptake in the central nervous system was confirmed by imaging the distribution of activity on the integrated living animal using a YAP(SSPECT small animal scanner.Uma nova classe de complexos de Tc-99m capazes de atravessar a barreira hemato-encefálica foi investigada e descrita neste trabalho. Estes compostos são formados reagindo os precursores bissubstituídos do nitrido [99mTc(N(PS2] (PS = phosphino-tiol ligante com trietillborano (BEt3 sob restritas condições anidras e usando solventes não aquosos. A estrutura molecular desses traçadores não foi totalmente estabelecida, mas evidências experimentais preliminares sugerem que eles resultam da interação da base de Lewis [99mTc(N(PS2] com o ácido de Lewis BEt3, levando a formação do novo aducto -B-Nº Tc-. Após purificação e recuperação em uma solução fisiológica, os novos derivados borano-nitrido-Tc-99m foram injetados em ratos para avaliação de seu comportamento biológico in vivo. Os resultados mostraram uma acumulação significativa no tecido cerebral

  2. Comparison of relative renal function measured with either 99m Tc-DTPA or 99m Tc-EC dynamic scintigraphies with that measured with 99m Tc-DMSA static scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Domingues, F.C.; Fujikawa, G.Y.; Decker, H.; Alonso, G.; Pereira, J.C.; Duarte, P.S. [Centro de Diagnostico Fleury, Sao Paulo, SP (Brazil). Secao de Medicina Nuclear; Sao Paulo Univ. (USP), SP (Brazil). Escola de Saude Publica. Dept. de Epidemiologia]. E-mail: paulo.duarte@fleury.com.br

    2006-07-15

    Objective: The aim of this study was to compare the renal function measured with either {sup 99m}Tc-DTPA or {sup 99m}Tc-EC dynamic scintigraphies with that measured using {sup 99m}Tc-DMSA static scintigraphy. Methods: the values of relative renal function measured in 111 renal dynamic scintigraphies performed either with {sup 99m}Tc-DTPA (55 studies) or with {sup 99m}Tc-EC (56 studies) were compared with the relative function measured using {sup 99m}Tc-DMSA static scintigraphy performed within a 1-month period. The comparisons were performed using Wilcoxon signed rank test. The number of {sup 99m}Tc-DTPA and {sup 99m}Tc-EC studies that presented relative renal function different by more than 5% from that measured with {sup 99m}Tc-DMSA, using chi square test were also compared. Results: the relative renal function measured with {sup 99m}Tc-EC is not statistically different from that measured with {sup 99m}Tc-DMSA (p = 0.97). The relative renal function measured with {sup 99m}Tc-DTPA was statistically different from that measured using {sup 99m}Tc-DMSA, but with a borderline statistical significance (p = 0.05). The number of studies with relative renal function different by more than 5% from that measured with {sup 99m}Tc-DMSA is higher for the {sup 99m}Tc-DTPA scintigraphy (p 0.04) than for {sup 99m}Tc-EC. Conclusion: the relative renal function measured with {sup 99m}Tc-EC dynamic scintigraphy is comparable with that measured with {sup 99m}Tc-DMSA static scintigraphy, while the relative renal function measured with {sup 99m}Tc-DTPA dynamic scintigraphy presents a significant statistical difference from that measured with {sup 99m}Tc-DMSA static scintigraphy. (author)

  3. Comparison of relative renal function measured with either 99mTc-DTPA or 99mTc-EC dynamic scintigraphies with that measured with 99mTc-DMSA static scintigraphy

    Directory of Open Access Journals (Sweden)

    F. C. Domingues

    2006-08-01

    Full Text Available OBJECTIVE: The aim of this study was to compare the renal function measured with either 99mTc-DTPA or 99mTc-EC dynamic scintigraphies with that measured using 99mTc-DMSA static scintigraphy. METHODS: the values of relative renal function measured in 111 renal dynamic scintigraphies performed either with 99mTc-DTPA (55 studies or with 99mTc-EC (56 studies were compared with the relative function measured using 99mTc-DMSA static scintigraphy performed within a 1-month period. The comparisons were performed using Wilcoxon signed rank test. The number of 99mTc-DTPA and 99mTc-EC studies that presented relative renal function different by more than 5% from that measured with 99mTc-DMSA, using chi square test were also compared. RESULTS: the relative renal function measured with 99mTc-EC is not statistically different from that measured with 99mTc-DMSA (p = 0.97. The relative renal function measured with 99mTc-DTPA was statistically different from that measured using 99mTc-DMSA, but with a borderline statistical significance (p = 0.05. The number of studies with relative renal function different by more than 5% from that measured with 99mTc-DMSA is higher for the 99mTc-DTPA scintigraphy (p = 0.04 than for 99mTc-EC. CONCLUSION: the relative renal function measured with 99mTc-EC dynamic scintigraphy is comparable with that measured with 99mTc-DMSA static scintigraphy, while the relative renal function measured with 99mTc-DTPA dynamic scintigraphy presents a significant statistical difference from that measured with 99mTc-DMSA static scintigraphy.

  4. Lung fluid clearance in chronic heart failure patients.

    Science.gov (United States)

    Dixon, Dani-Louise; De Pasquale, Carmine G; Lawrence, Mark D; Cavallaro, Elena; Rubino, Vito; Bersten, Andrew D

    2017-10-01

    Chronic elevation of pulmonary microvascular pressure (Pmv) consistently leads to alveolocapillary barrier thickening and reduction in the filtration coefficient. In animal models of chronic heart failure (CHF) the lung remains dry despite hydrostatic forces. As fluid flux is bi-directional, it has been postulated that an increase in alveolar fluid clearance may facilitate the dry lung when Pmv is chronically elevated. In this study we aimed to examine alveolar fluid clearance in ambulatory patients with CHF secondary to left ventricular (LV) systolic dysfunction compared against non-CHF controls. Lung clearance following aerosol delivery of (99m)technetium (Tc)-diethyl triaminepentaacetic acid (DTPA) was measured non-invasively by scintigraphy and half time of (99m)Tc-DTPA clearance (T (1/2)) was calculated by mono-exponential curve fit. Alveolar fluid clearance measured as half time DTPA clearance was significantly faster in CHF patients than controls (P=0.001). This was further defined by NYHA classification. No correlation was found between DTPA clearance and plasma epinephrine, norepinephrine or aldosterone hormone (P>0.05). Our results support an association between increasing alveolar fluid clearance and disease severity in CHF, and the concept of controlled bi-directional fluid flux in CHF associated with increasing Pmv, and represents another defence mechanism of the lung against pulmonary oedema. Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.

  5. Technetium-99m tetrofosmin imaging in thyroid diseases: comparison with Tc-99m-pertechnetate, thallium-201 and Tc-99m-methoxyisobutylisonitrile scans

    Energy Technology Data Exchange (ETDEWEB)

    Klain, M. [Centro di Studio per la Medicina Nucleare, Consiglio Nazionale delle Ricerche (CNR) Napoli (Italy)]|[Univ. di Napoli (Italy). Medicina Nucleare; Maurea, S. [Medicina Nucleare, Istituto Nazionale dei Tumori, Napoli (Italy); Cuocolo, A. [Centro di Studio per la Medicina Nucleare, Consiglio Nazionale delle Ricerche (CNR) Napoli (Italy)]|[Univ. di Napoli (Italy). Medicina Nucleare; Colao, A. [Endocrinologia, Centro di Studio per la Medicina Nucleare del Consiglio Nazionale delle Ricerche (CNR), Napoli (Italy)]|[Univ. di Napoli (Italy); Marzano, L. [Endocrinologia, Centro di Studio per la Medicina Nucleare del Consiglio Nazionale delle Ricerche (CNR), Napoli (Italy)]|[Univ. di Napoli (Italy); Lombardi, G. [Endocrinologia, Centro di Studio per la Medicina Nucleare del Consiglio Nazionale delle Ricerche (CNR), Napoli (Italy)]|[Univ. di Napoli (Italy); Salvatore, M. [Centro di Studio per la Medicina Nucleare, Consiglio Nazionale delle Ricerche (CNR) Napoli (Italy)]|[Univ. di Napoli (Italy). Medicina Nucleare

    1996-12-01

    In this study, tetrofosmin whole-body scintigraphy was performed in 35 patients with evidence of thyroid diseases. All patients underwent laboratory evaluation of thyroid function as well as {sup 99m}Tc pertechnetate scan, thallium-201 {sup 99m}Tc-methoxyisobutylisonitrile (MIBI) whole-body studies. Thyroid images were semi-quantitatively analysed by a 4-point score: 0=no significant uptake; 1=uptake increased but inferior to normal thyroid tissue; 2=uptake equal to normal thyroid tissue; 3=uptake superior to normal thyroid tissue. A total of 41 thyroid nodules were detected, of which 15 were goitre nodules, 13 adenomas and 13 malignant lesions. In goitre nodules, concordant results of tetrofosmin and pertechnetate uptake (score 1 or 0) were observed in the majority of lesions (87%). In function adenomas both tetrofosmin uptake and pertechnetate uptake were score 3. In non-function adenomas tetrofosmin uptake was score 3, while pertechnetate uptake was score 0. In six malignant lesions, tetrofosmin uptake was score 3, while pertechnetate uptake was score 0; in the other seven lesions, where a prevalence of goitre abnormalities was observed, results of tetrofosmin and pertechnetate uptake were similar (score 0 or 1). In (70%) patients with malignant nodules, whole-body tetrofosmin images showed increased abnormal uptake in a total of 28 extra-thyroid tumour sites, as subsequently confirmed by other techniques. When tetrofosmin images were compared to {sup 201}Tl and {sup 99m}Tc-MIBI scans, concordant results were observed in all cases. In conclusion, tetrofosmin imaging may be particularly useful to characterize and stage patients with malignant thyroid nodules; it shows similar results to thallium but provides better image quality. Comparable findings were observed between tetrofosmin and MIBI studies. Thus, tetrofosmin may be an alternative to thallium and MIBI in the aforementioned patients. (orig.). With 4 figs., 3 tabs.

  6. Temporal change in regional brain distribution of the technetium-99m ethyl cysteinate dimer

    Energy Technology Data Exchange (ETDEWEB)

    Ueda, Osamu; Ashihara, Tatsuhiko; Wake, Seiji; Tsuru, Masato; Izawa, Ichiro; Takahashi, Ryuji (Himeji Cardiovascular Center, Hyogo (Japan)); Kida, Tohru; Nakade, Takahide

    1993-09-01

    To determine the data acquisition timing on the SPECT of the brain using Technetium-99m ethyl cysteinate dimer ([sup 99m]Tc-ECD), the changes in the brain distribution of the tracer were examined in 10 patients with cerebrovascular disease (CVD) and 4 patients with neurological degenerative disease. Immediately after I.V. administration of [sup 99m]Tc-ECD with a dose of 740 MBq, the dynamic planar images (5 sec/frame x 60 frames) and consecutive early 6 SPECT images (5 sec/view x 64 views each) were obtained. The delayed SPECT images (30 sec/view x 64 views) were taken 3 hr after injection. The following parameters were evaluated: (1) the whole brain time-activity curve of [sup 99m]Tc-ECD; (2) the count of the region of interest (ROI) placed in the frontal lobe, temporal lobe, occipital lobe, parietal lobe, and cerebellum; (3) the count ratio of the lesion to the counterpart of the unaffected side, and to the ipsilateral cerebellum in CVD patients; and (4) the changes in the intra-cranial background and the extra-cranial background, where the former was expressed as the count ratio of the ventricle to gray matter and the latter as the count ratio of the peri-cranial regions to the whole brain. The whole brain time-activity curves of [sup 99m]Tc-ECD demonstrated a rapid raise in the uptake after the tracer injection and a subsequent plateau 3 min after the injection. A washout of [sup 99m]Tc-ECD was minimal in all ROIs during the period on the early 6 SPECT images. The count ratio of the lesion to the normal areas was constant on both early and delayed SPECT images. The intra-cranial background activity was unchanged during the whole periods, while the extra-cranial background activity reduced with time. These results suggested that the data acquisition of brain SPECT with [sup 99m]Tc-ECD could be initiated 5 min after the tracer injection. (author).

  7. Evaluation of flow characteristics of soft-tissue vascular malformations using technetium-99m labelled red blood cells

    Energy Technology Data Exchange (ETDEWEB)

    Inoue, Yusuke; Yoshikawa, Kohki; Yoshioka, Naoki; Ohtake, Tohru; Ohtomo, Kuni [Tokyo Univ. (Japan). Dept. of Radiology; Wakita, Shinichi; Kaji, Nobuyuki; Harii, Kiyonori [Dept. of Plastic Surgery, Univ. of Tokyo (Japan)

    1999-04-29

    The estimation of intralesional haemodynamics is crucial in determining appropriate treatment for soft-tissue vascular malformations. The aim of this study was to develop a method to evaluate the flow characteristics of soft-tissue vascular malformations using technetium-99m labelled red blood cells ({sup 99m}Tc-RBCs). Seventy-nine soft-tissue vascular malformations, including 20 arteriovenous malformations and 59 venous malformations, in 57 patients were examined. Following the intravenous injection of {sup 99m}Tc-RBCs, dynamic imaging was performed for 30 min with the lesion in the field of view ({sup 99m}Tc-RBC flow study). A time-activity curve was generated for the lesion, and the lesion was categorized as a high-flow or low-flow lesion by visual inspection of the curve. In low-flow lesions, mean vascular transit time (MTT) was calculated by curve fitting based on a two-compartment model. Twenty-nine lesions in 19 patients were examined twice, and reproducibility was assessed. In 23 venous malformations in 16 patients, {sup 99m}Tc-Sn colloid was percutaneously injected into the intravascular space of the lesion, and dynamic data of 5-min duration were acquired (direct puncture scintigraphy). MTT was estimated from the washout curve and compared with MTT estimated by {sup 99m}Tc-RBC flow study. {sup 99m}Tc-RBC flow study classified all 20 arteriovenous malformations as high-flow lesions and all 59 venous malformations as low-flow lesions. In the low-flow lesions, MTT estimated by {sup 99m}Tc-RBC flow study ranged from 61.2 to 2174.9 s. In the reproducibility study, complete concordance in classification and high correlation in MTT were shown between the first and second examinations. MTT estimated by {sup 99m}Tc-RBC flow study was significantly correlated with that estimated by direct puncture scintigraphy. In summary, {sup 99m}Tc-RBC flow study provides a quantitative indicator of intralesional haemodynamics in low-flow lesions in addition to accurate

  8. Osteomyelitis diagnosis by {sup 99m}Tc radiolabeled aptamers

    Energy Technology Data Exchange (ETDEWEB)

    Santos, S.R.; Ferreira, I.M.; Andrade, A.S.R., E-mail: sararoberta7@hotmail.com, E-mail: imendesf@yahoo.com.br, E-mail: antero@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Barros, A.L.B.; Cardoso, V.N.; Diniz, O.F., E-mail: brancodebarros@yahoo.com.br, E-mail: valbertcardoso@yahoo.com.br, E-mail: simoneodilia@yahoo.com.br [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Faculdade de Farmacia. Departamento de Analises Clinicas e Toxicologicas

    2015-07-01

    Osteomyelitis, which is characterized by progressive inflammatory destruction and new opposition of bone, is still a difficult infection to treat. The clinical diagnosis in late stages is achieved easily, but an early diagnosis is more challenging. Staphylococcus aureus is a common agent found in osteomyelitis and bone prostheses infection. Diagnosis by scintigraphy has advantages because it is a non-invasive procedure and is able to perform an early diagnosis even before anatomic changes. Thus, nuclear medicine could contribute to an accurate diagnosis since specific radiopharmaceuticals were developed. In this study, aptamers selected to Staphylococcus aureus were labeled with {sup 99m}Tc and used for bacteria identification in an osteomyelitis experimental model. The aptamers selected to S. aureus were directly labelled with {sup 99m}Tc and were evaluated by biodistribution studies. Wistar rats with intraosseous infection in the right paw were used. A random aptamer labelled with {sup 99m}Tc was as control. Six animals were used in each group. The aptamers labeled with {sup 99m}Tc were able to identify the infection foci caused by S. aureus displaying a target/non-target ratio of 2,23 ± 0,20, after 3 h. The control group presented a target/non-target ratio 1,08 ± 0.23. The results indicated that the radiolabeled aptamers were able to identify specifically the infection foci and they should be further explored for infection diagnosis by scintigraphy. (author)

  9. Evaluation of myocardial revascularization utilizing Tc-99m pyrophosphate.

    Science.gov (United States)

    Liddicoat, J E; Bekassy, S M; Johnson, P C; Ajmani, S K; DeBakey, M E

    1974-01-01

    Early clinical application of technetium 99m labeled pyrophosphate for cardiac imaging has revealed a safe noninvasive method of determining areas of impaired myocardial perfusion in patients with atherosclerotic occlusive coronary artery disease. Using this method, we were able to demonstrate improvement in myocardial blood supply following coronary artery bypass surgery.

  10. Development of Tc-99m Imaging Agents for Abeta Plaques

    Energy Technology Data Exchange (ETDEWEB)

    Zhi-Ping, Zhuang; Mei-Ping Kung; Catherihne Hou; Hank F. Kung

    2008-09-26

    Development of SPECT imaging agents based on Tc-99m targeting Aβ plaques is useful for diagnosis of Alzheimer’s disease (AD). A stilbene derivative, [11C]SB-13, showing promise in detecting senile plaques present in AD patients has been reported previously1,2. Based on the 4’-amino-stilbene core structure we have added substituted groups through which a chelating group, N2S2, was conjugated. We report herein a series of Tc-99m labeled stilbene derivative conjugated with a TcO[N2S2] core. The syntheses of stilbenes containing a N2S2 chelating ligand are achieved by a scheme shown. Lipophilic 99mTc stilbene complexes were successfully prepared and purified through HPLC. Preliminary results of in vitro labeling of brain sections from transgenic mice showed very promising plaque labeling. These 99mTc stilbene derivatives are warranted for further evaluations as potential imaging agents targeting amyloid plaques.

  11. Technetium-99m direct radiolabeling of Lanreotide: a Somatostatin analog

    Energy Technology Data Exchange (ETDEWEB)

    Pervez, Shahid; Mushtaq, A. E-mail: pinstech@paknet2.ptc.pk; Arif, Muhammad

    2001-11-01

    Lanreotide, a synthetic octapeptide analog of a native hormone somatostatin, was labeled with a commonly available, inexpensive radionuclide {sup 99m}Tc. Labeling was accomplished by reduction of the cysteine bridge, which provided sulfhydryl groups for chelation with {sup 99m}Tc. Stannous chloride was used as reducing agent, while tartrate acted as transchelating agent. Lanreotide (100 {mu}g), stannous chloride dihydrate (100 {mu}g) and tartaric acid (64 {mu}g) were dissolved in acetate/acetic acid buffer (pH 2.8). After overnight ({approx}18 h) incubation, {approx}444 MBq (12 mCi) {sup 99m}Tc was added and kept in boiling water for 30 min. More than 97% labeling efficiency was confirmed by RP-HPLC, ITLC-SG and C{sub 18} cartridge analysis. Radiolabeling results in one major peak when analyzed by reverse-phase HPLC. The stability of the {sup 99m}Tc-peptide bond was evaluated by cysteine challenge studies.

  12. 99m Tc-labeled heparin test in orthopaedic surgery

    Energy Technology Data Exchange (ETDEWEB)

    Bouvier, J.F.; Lafon, J.C.; Colin, M.; Chatelut, J.; Beaubatie, F. (Hopital Universitaire Dupuytren, Limoges (France))

    1983-06-30

    99m Tc-labeled heparin test was performed for early detection of phlebitis or pulmonary embolism after orthopaedic prothesis. Heparinic treatment and surgery per se were demonstrated to have no effect on the results. If this test demonstrates a statistical difference for pathologic patients, it is of greater value to consider ratio between rates before and after intervention.

  13. Technetium-99m diethylenetriaminepentaacetic acid radioaerosol scintigraphy in organophosphate induced pulmonary toxicity: experimental study.

    Science.gov (United States)

    Yavuz, Yucel; Kaya, Eser; Yurumez, Yusuf; Sahin, Onder; Bas, Orhan; Fidan, Huseyin; Sezer, Murat

    2008-09-01

    The aim of this experimental study was to investigate pathological signs of lung damages caused by acute organophosphate (OP) poisoning by using Tc-99m DTPA radioaerosol scintigraphy and histopathological investigation. Fourteen rabbits were divided into two equal groups (n = 7). Group 1 (control group) received normal saline (same volume of fenthion, 2 ml/kg) via orogastric tube. Group 2 (OP toxicity group) received 150 mg/kg of fenthion (diluted fenthion, 2 ml/kg) via orogastric tube. Six hours later, Tc-99m-DTPA aerosol inhalation lung scintigraphy was performed in both groups. Then all rabbits were anesthetized with ketamine hydrochloride (35 mg/kg, i.p.) and xysilazine (5 mg/kg, i.p.), and sacrificed by intracardiac blood discharge. The lungs were then removed. There was a significant difference in T1/2 values of Tc-99m DTPA clearance between control group and OP toxicity group (p = 0.04). Intraparenchymal vascular congestion and thrombosis, intraparenchymal hemorrhage, respiratory epithelial proliferation, number of macrophages in the alveolar, and bronchial lumen, alveolar destruction, emphysematous changes, and bronchoalveolar hemorrhage scores were significantly higher in the rabbits exposed to OP compared with the control group (p lung scintigraphy was found to be a sensitive determination of acute lung damage in OP poisoning.

  14. Revision: quality control of {sup 99m}Tc; Revisao: controle da qualidade do {sup 99m}Tc

    Energy Technology Data Exchange (ETDEWEB)

    Alves, S.M.; Kabad, M.C. [Universidade Estacio de Sa (UESA), Rio de Janeiro, RJ (Brazil); Teixeira, G.J.; Sousa, C H.S., E-mail: sanny.alves@yahoo.com.br [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

    2015-07-01

    The radiopharmaceutical most commonly used in nuclear medicine is technetium-99m. Radiopharmaceuticals labeled with {sup 99m}Tc are the main agents for diagnosis, being used in the vast majority of procedures due to radionuclide characteristics. During its preparation impurities can be generated that make products with different qualities of the desired. In this paper were presented some of the factors that can interfere with radiopharmaceuticals and the tests used for quality assurance, certifying that the product is fit for purpose. It is up to nuclear medicine centers deploy a program to raise the quality of exams and increase patient safety. (author)

  15. Joint scintigraphy using technetium-99m pyrophosphate in experimental hemarthrosis

    Energy Technology Data Exchange (ETDEWEB)

    Salimi, Z.; Vas, W.; Restrepo, G.

    1986-02-01

    To determine the validity of a method for induction of experimental hemarthrosis in dogs and for the nuclear imaging of hemarthrosis, serial technetium-99m pyrophosphate ((/sup 99m/Tc)PYP) flow and blood-pool scans were performed monthly in eight dogs who received bi-weekly injections of autologous blood into their femoro-tibial joints (also called stifle joint). In four control dogs, one joint was injected with saline while the other joint received only a sham injection. In addition, two dogs received intra-articular injections of autologous blood into their right stifle joint and saline into their left stifle joint. These dogs were studied with /sup 99m/TcO/sub 4/ joint scintigraphy at monthly intervals. The dogs were periodically taken out of the study and explored surgically. Pathologic examination of synovial tissue was performed. Serial radiographs were also obtained and correlated with the scan and surgical findings. There was a striking abnormal increase in blood-pool activity of (/sup 99m/Tc)PYP in the treated stifle joints, commencing at the first examination after 1 mo of blood injections and continuing for the length of the study. All radiographs showed only minimal joint space widening and some soft-tissue swelling. On pathologic examination, both grossly and microscopically, there was profuse pannus formation, with intense inflammatory infiltrate replacing much of the subsynovial fat. The scintigraphic findings correlated well with these pathologic findings. This study not only validates this method for simulating hemophilic hemarthrosis but also suggests that (/sup 99m/Tc)PYP joint scintigraphy is a simple, and noninvasive method for monitoring the early changes in hemophilic arthropathy and is superior to pertechnetate imaging for this disease process.

  16. Detection of melanoma metastases with Tc-99m-tetrofosmin; Nachweis von Melanommetastasen mit Tc-99m-Tetrofosmin

    Energy Technology Data Exchange (ETDEWEB)

    Rettenbacher, L.; Kaessmann, H.; Galvan, G. [Inst. fuer Nuklearmedizin und Endokrinologie, Landeskrankenanstalten Salzburg (Austria); Koller, J. [Abt. fuer Dermatologie, Landeskrankenanstalten Salzburg (Austria)

    2000-06-01

    Aim: The aim of this study was to evaluate Tc-99m-tetrofosmin whole-body imaging in the detection of metastases in patients with malignant melanoma. Methods: In 30 patients with suspected melanoma metastases we performed whole body imaging. After administration of about 600 MBq Tc-99m-tetrofosmin dynamic images up to 10 min were performed in 7 patients (1 image per 10 sec) to evaluate the optimal tracer uptake in the metastases. In all patients whole-body images were performed 5-10 min p.i. using an acquisition time of 5 min per image. The final diagnosis was confirmed by surgical histology in 30 lesions, by computertomography and clinical course in the remaining lesions. Results: Out of 64 melanoma metastases 49 were detected using Tc-99m-tetrofosmin scintigraphy (49 rp., 15 fn.). The overall sensitivity for the detection of malignant lesions was 77%. Referring only to the lymph node metastases, the sensitivity was 87% (26 rp; 4fn.). The maximal tracer uptake was reached 1 min after injection, with a slow decrease in the following 10 minutes. The size of the lesions ranged between 0.5 and 7.0 cm and the T/B ratios between 1.3 and 3.0 (mean 1.88). Conclusions: Tc-99m-tetrofosmin whole body imaging is a simple and side-effect-less method for the detection of melanoma metastases especially of lymph node metastases. The results are comparable to Tc-99m-sestamibi and Tl-201 scintigraphy. (orig.) [German] Ziel: Ziel dieser Studie war es, festzustellen, wieweit sich die Tc-99m-Tetrofosminszintigraphie zum Nachweis von Melanommetastasen eignet. Methoden: Bei 30 Patienten mit suspekten Melanommetastasen wurde eine Tc-99m-Tetrofosmin Ganzkoerperszintigraphie durchgefuehrt. Nach intravenoeser Injektion von 600 MBq Tc-99m-Tetrofosmin erfolgten bei 7 Patienten Sequenzszintigraphien ueber 10 Min. (1 Bild pro 10 Sek.), um den optimalen Traceruptake im Tumor festzustellen und bei allen Patienten Ganzkoerperaufnahmen (Aufnahmebeginn 5-10 min p.i., Aufnahmezeit 5 Min. pro Bild). 30

  17. Clinical experience with technetium-99m teboroxime, a neutral, lipophilic myocardial perfusion imaging agent

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, L.L.; Seldin, D.W. (Columbia Univ., New York, NY (USA))

    1990-10-16

    Technetium-99m (Tc-99m) teboroxime is a new technetium-based myocardial perfusion imaging agent (investigational code = SQ30217 (Cardiotec, Squibb Diagnostics)). A member of a class of neutral, lipophilic, technetium-containing complexes known as boronic acid adducts of technetium dioxime (BATO) complexes, this agent is chemically very different from the cationic tracer thallium-201 (Tl-201) and from the cationic technetium complex Tc-99m sestamibi (Cardiolite, Du Pont Imaging Agents). Tc-99m teboroxime has high myocardial extraction, rapid blood clearance, little lung uptake and rapid myocardial washout. A biexponential pattern of myocardial washout is demonstrated in animals and in man. Effective half-lives of the 2 washout components in man are 5.2 minutes and 3.8 hours and represent approximately 66 and 33% of the myocardial activity, respectively. The first half-life for the myocardium is approximately 11 minutes. As the agent washes out of the heart, hepatic uptake occurs, peaking at about 5 minutes after injection. The liver is the major organ of excretion and receives, along with the large bowel, the largest radiation dose. Rapid imaging protocols using standard cameras have achieved good myocardial counts from 3 planar views acquired over a 4- to 5-minute period or for single photon emission computed tomography (SPECT) images acquired over a 10-minute period. An entire stress/rest procedure can be completed in 1 hour. Analysis of data from 155 patients from 4 centers using planar or SPECT imaging showed a sensitivity and specificity for blinded readings of 82 and 91%, respectively, when compared against overall clinical impression. 13 references.

  18. Preclinical evaluation of technetium 99m-labeled P1827DS for infection imaging and comparison with technetium 99m IL-8.

    NARCIS (Netherlands)

    Krause, S.; Rennen, H.J.J.M.; Boerman, O.C.; Baumann, S.; Cyr, J.E.; Manchanda, R.; Lister-James, J.; Corstens, F.H.M.; Dinkelborg, L.M.

    2007-01-01

    BACKGROUND: The technetium 99 m (99mTc)-radiolabeled, leukocyte-avid peptide-glycoseaminoglycan complex, [99mTc]P1827DS, has been synthesized as an improved infection/inflammation imaging agent to [99mTc]P483H (LeukoTect, Diatide). In a phase I/II clinical trail, [99mTc]P483H images were equivalent

  19. Tc-99m ciprofioxacin imaging in acute cholecystitis

    Institute of Scientific and Technical Information of China (English)

    Yun-Mee Choe; Kee-Chun Hong; Seok-Hwan Shin; Kyung-Rae Kim; Ze-Hong Woo; Wonsick Choe; Keon-Young Lee; Seung-Ik Ahn; Kwang Kim; Young Up Cho; Sun Keun Choi; Yoon-Seok Hur; Sei-Joong Kim

    2007-01-01

    AIM: To evaluate the efficacy of a new nuclear imaging Infecton (Tc-99m ciprofioxacin) for the diagnosis of acute cholecystitis.METHODS: Sixteen patients thought to have acute cholecystitis were included in this study. The diagnosis of acute cholecystitis was made based on clinical symptoms and ultrasonographic and pathologic findings.RESULTS: The 16 patients were composed of 12 acute and 4 chronic cholecystitis patients. Twelve patients with acute cholecystitis were image-positive, including one false-positive. Four patients with chronic cholecystitis were image-negative, of whom three were true-negative.This nuclear imaging had a sensitivity of 91.7%, a specificity of 75%, a positive-predictive value of 91.7%,and a negative-predictive value of 75%.CONCLUSION: Tc-99m ciprofloxacin imaging is easy to perform and applicable for the diagnosis of acute cholecystitis.

  20. Technetium-99m DMSA preparation: Trivial issues causing severe problems

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, V. [Westmead and The Children`s Hospital, Westmead, NSW (Australia)

    1997-12-01

    Urinary tract infection (UTI) in children involving renal parenchyma, upper collecting system or bladder is one of the major causes for consideration in the diagnosis and management of paediatric nuclear medicine. Acute pyelonephritis is one of the prime causes of morbidity associated with urinary tract infection in children which can lead to progressive renal damage. Technetium-99m dimercaptosuccinic acid (DMSA) is used extensively for the assessment of UTI in paediatrics. The radiopharmaceutical preparation could be influenced by several factors, most of them are trivial, but invariably have severe impact on the quality of the scintiphotographs. This communication is mainly to highlight some of the issues related to {sup 99}mTc-DMSA preparation and the possible precautionary measures that need to be taken to obviate unwarranted problems. (author) 2 refs., 4 figs.

  1. Development and biodistrubition modeling of 99mTc-DTPA

    Directory of Open Access Journals (Sweden)

    Mohammed Bricha

    2015-09-01

    Full Text Available Purpose: In this study, the team modeled the biodistribution and the efficiency of two 99m-technetium diethylene triamine penta acetate (99mTc-DTPA based radiopharmaceuticals.Methods: The first radiopharmaceutical (DTPA-CNESTEN is developed at the laboratories of the radiopharmaceutical production unit of the National Center for Nuclear Energy, Sciences and Technologies (CNESTEN-Morocco, and the second one is the commercial DTPA (DTPA-ref. Freeze-dried kits were successfully radiolabeled (radiochemical purity >95% with the 99m Tc. Then drugs were injected to male BALB/c mice. In each 2 min, 5 min, 15 min, 1 h and 2 h time points after injections we evaluate tissue’s distributions characteristics. At the end, an automatic modeling of the data were recorded from thyroid, blood and urinary excretion kinetics and biodistribution in mice using both DTPA kits. The study aimed to extract the parameters of the function used to fit the recorded data. Results and Conclusion: the team concluded that the biodistribution of 99mTc-DTPA can be modeled using a combination of two exponential parts. Moreover, the resultant plots showed that there is strong correlation between the formula found in literature and the one derived on the basis of the fit of data sets in this study. In addition, it was found that the biodistribution behaviors of the developed kit and the commercial one were very close. The obtained results suggest that the developed DTPA has practically the same kinetics as the commercial one.

  2. Evaluation of RI images of hepatic blood flow using Tc-99m PMT

    Energy Technology Data Exchange (ETDEWEB)

    Fujiwara, Hiromichi; Iwasaki, Naoya; Ichikawa, Kesato

    1988-03-01

    To evaluate the clinical significance of RI images of hepatic blood flow using Tc-99m-PMT, analysis of the RI images and estimation of hepatic blood flow were carried out in patients with various liver diseases (37 cases). After intravenous injection of Tc-99m-PMT, hepatic accumulation curve of ROI positioned at whole liver area and time activity curve of ROI positioned at celiac artery were obtained through scintillation camera images with the computer-analysed system. Hepatic blood flow coefficient (K) was calculated from the hepatic accumulation curve. Based on the differential curve calculated from the time activity curve, chronological images of arterial, portal, parenchymal and saturated parenchymal phases were obtained. Results ; 1) K was 0.50 + 0.04, 0.35 + 0.02 and 0.26 + 0.04/min in normal type, CH type and LC type respectively. These coefficients well correlated with clinical severity of hepatic diseases. 2) Perfusional phase images of the liver became poorer in accordance with progression of liver disease, while images of spleen, portal venous system and collateral channels were more clearly obtained in liver cirrhosis. This method was shown to have a potential to understanding of severity of liver disease and hepatic blood flow dynamics.

  3. Tc-99m-tetrofosmin scintigraphy: An alternative scintigraphic method for following up differentiated thyroid carcinoma - preliminary results

    Energy Technology Data Exchange (ETDEWEB)

    Gallowitsch, H.J. [LKH Klagenfurt (Austria). Dept. of Nuclear Medicine and Special Endocrinology; Kresnik, E. [LKH Klagenfurt (Austria). Dept. of Nuclear Medicine and Special Endocrinology; Mikosch, P. [LKH Klagenfurt (Austria). Dept. of Nuclear Medicine and Special Endocrinology; Pipam, W. [LKH Klagenfurt (Austria). Dept. of Nuclear Medicine and Special Endocrinology; Gomez, I. [LKH Klagenfurt (Austria). Dept. of Nuclear Medicine and Special Endocrinology; Lind, P. [LKH Klagenfurt (Austria). Dept. of Nuclear Medicine and Special Endocrinology

    1996-12-01

    Aim: The usefulness of the myocardial perfusion agent, Tc-99m-tetrofosmin (Myoview{sup R}) in the follow-up of differentiated thyroid carcinoma was evaluated in a clinical study of 15 patients, primary treated with thyroidectomy and high-dose I-131-therapy (2960-3700 MBq), 12 with suspected recurrence and metastases and three patients without any suspicion and compared with other non-specific tracers like Tl-201 and Tc-99m-sestamibi. Method: Twelve patients with elevated thyroglobulin (Tg) levels of more than 10 ng/ml (group A), four of these had negative I-131 scans, and three patients with Tg levels less than 10 ng/ml (group B) were examined under TSH suppressive L-Thyroxine treatment. Whole body scans were taken with Tl-201 (74 MBq; 20 min post injection), Tc-99m-sestamibi (370 MBq; 20-60 min post injection) and Tc-99m-tetrofosmin (370 MBq; 20-60 min post injection). Tumor/background ratios and optional time/activity analyses (up to 150 min post injection) were evaluated using the region of interest approach. Results: Compared with Tl-201 (T/BG: 1.59{+-}0.396), Tc-99m-tetrofosmin showed slightly but not significant better T/BG ratios and detection rates (T/BG: 1.76, {+-}0.345). Tc-99m-sestamibi (1.51, {+-}0.31 p=0.05) showed significantly lower values than Tc-99m-tetrofosmin. Conclusion: In the light of these results, scintigraphy with Tc-99m-tetrofosmin seems to be a possibly sensitive imaging modality in the follow-up of DTC with possible advantages concerning T/Bg ratio, background clearance, detection rate and dosimetry compared with Tl-201 and Tc-99m-sestamibi, especially in patients with elevated Tg level and no iodine uptake, but further investigations are needed to confirm our preliminary results. (orig.) [Deutsch] Ziel: Die Verwendbarkeit des myokardialen Perfusionstracers Tc-99m-Tetrofosmin (Myoview{sup R}) fuer die Nachsorge des differenzierten Schilddruesenkarzinoms wurde in einer Pilotstudie an primaer operierten und radiojodtherapierten Patienten

  4. Evaluation of anti-peptidoglycan aptamers labeled with Technetium-99m for in vivo bacterial infection identification; Avaliacao de aptameros anti-peptidoglicano marcados com Tecnecio-99m para identificacao in vivo de infecoes bacterianas

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, Ieda Mendes

    2017-07-01

    Aptamers are oligonucleotides that display high affinity and specificity for their molecular targets and are emerging as promising molecules for radiopharmaceuticals development. In a previous work, we selected two aptamers for peptidoglycan (the main constituent of bacterial cell walls) termed Antibac1 and Antibac2. In the present study, the characterization of these aptamers was completed, and the dissociation coefficients (K{sub d}) were determined. The aptamers were further labeled with {sup 99m}Tc and evaluated for bacterial infection diagnosis by scintigraphy. The K{sub d} obtained for Antibac1 was of 0.415 ± 0.047 μM and for Antibac2 of 1.261 ± 0.280 μM. The direct labeling method with {sup 99m}Tc allowed radiolabel yields higher than 90% and the radiolabel stability in saline, plasma and cysteine excess indicated that the process was suitable for labeling of both aptamers. The {sup 99m}Tc-aptamers are prone to bind to plasma proteins: 39.5% ± 2.9% (1 h) and 43.6% ± 1.2% (3 h) for {sup 99m}Tc-Antibac1; 37.6% ± 2.0% (1 h) and 40.9% ± 0% (3 h) for {sup 99m}Tc-Antibac2. The blood clearance half-life for {sup 99m}Tc-Antibac1 was of 41.26 min and for the {sup 99m}Tc-Antibac2 of 31.58 min. The {sup 99m}Tc-Antibac1 in the group infected with S. aureus presented a target/non-target ratio of 2.81 ± 0.67, significantly higher than verified for the {sup 99m}Tc-library (control): 1.52 ± 0.07. In the model with C. albicans infection the target/non-target ratio for {sup 99m}Tc-Antibac1 was 1.46 ± 0.11, similar that obtained for the {sup 99m}Tc-library in the same model: 1.52 ± 0.05. The {sup 99m}Tc-Antibac2 in the group infected with S. aureus showed a target/non-target ratio of 2.61 ± 0.66, statistically higher than achieved for the {sup 99m}Tc-library in the same infection model: 1.52 ± 0.07. In the group infected with C. albicans this ratio for {sup 99m}Tc-Antibac2 was 1.75 ± 0.19, it was significantly higher than verified for the {sup 99m}Tc-library: 1

  5. Perfusion lymphoscintigraphy using sup 99m Tc-human serum albumin in patients with treated uterine cancer

    Energy Technology Data Exchange (ETDEWEB)

    Kataoka, Masaaki; Hamada, Katsuyuki; Hamamoto, Ken; Takeda, Yasunari; Matsuura, Shumpei (Ehime Univ., Matsuyama (Japan). School of Medicine); Kawamura, Masashi

    1990-09-01

    Perfusion lymphoscintigraphy was performed by subcutaneous injection of 7.4 MBq (0.2mCi) {sup 99m}Tc-human serum albumin ({sup 99m}Tc-HSA) on 18 patients with uterine cancer treated by operation and/or irradiation. Radioactivity at the injection site was counted for 3 min at 10 min (a) and at 3 hr (b) after injection, and the clearance of {sup 99m}Tc-HSA was defined as (1-(b)/(a)) x 100(%) ((a) and (b) were corrected for decay of the isotope). The clearance in 6 legs with lymphedema was significantly more delayed than that in 16 legs without lymphedema in the patients treated with both surgery and irradiation (16.6 +- 7.7% vs 34.9 +- 9.3%: P< 0.01). The clearances in edematous legs in a case which had developed a venous occulusion after operation were 44.2% and 41.7%, which were almost the same as those in the non-edematous patients treated with surgery alone. The clearance in patients treated with both operation and irradiation was significantly more delayed than that in patients treated with the signle modality of operation or irradiation (29.7 +- 11.9% vs 41.1 +- 7.2%, 44.5 +- 7.7%, respectively: P< 0.01). These data suggest that perfusion lymphoscintigraphy using {sup 99m}Tc-HSA is useful for evaluating patients with lymphedema and for differentiating it from edema caused by other mechanisms. (author).

  6. Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats

    Energy Technology Data Exchange (ETDEWEB)

    Acucena, Maria Kadja Meneses Torres; Pereira, Kercia Regina Santos Gomes; Villarim Neto, Arthur; Rego, Amalia Cinthia Meneses [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Centro de Ciencias da Saude; Bernardo-Filho, Mario [Universidade do Estado do Rio de Janeiro (UERJ), RJ (Brazil). Inst. de Biologia Roberto Alcantara Gomes. Dept. de Biofisica e Biometria; Azevedo, Italo Medeiros; Araujo Filho, Irami; Medeiros, Aldo Cunha [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Dept. de Cirurgia]. E-mail: aldo@ufrnet.br

    2008-12-15

    This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1 ml of 99mTc-DMSA IV (0.48 MBq) was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g) determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (p<0.05) was done. There was a significant reduction in %ATI/g in kidney and blood (p<0.05) of splenectomized animals, a significant increase (p<0.05) of urea (88.8 {+-} 18.6 mg/dL) and creatinine (0.56 {+-} 0.08 mg/dL), compared to the controls (51.5{+-}1.6, 0.37{+-}0.02 mg/dL, respectively), as well as increase in platelets and leucocytes, and hematocrit reduction. The analysis of the results indicates that in rats, splenectomy seems to alter the renal function and the uptake of 99mTc-DMSA. (author)

  7. Estimation of hepatic functional reserve by asialoglycoprotein receptor-binding, radiolabeled synthetic ligand 'Tc-99m-galactosyl-neoglycoalbumin'. Preclinical and clinical studies

    Energy Technology Data Exchange (ETDEWEB)

    Kudo, Masatoshi; Todo, Akio; Ikekubo, Katsuji

    1987-11-01

    Tc-99m-Galactosyl-Neoglycoalbumin (Tc-99m-NGA) is a receptor binding radiolabeled ligand, which binds to the asialoglycoprotein receptor of the plasma membrane of hepatocytes. NGA was prepared by covalently coupling D-galactose to normal human albumin. NGA was labeled with technetium-99m according to the electrolytic method by Benjamin, which produced Tc-99m-NGA with labeling yield in excess of 95%. Tc-99m-NGA was proved to be stable for at least 4 hours as determined by high performance liquid chromatography. Biodistribution studies of Tc-99m-NGA in normal rats demonstrated that liver is a sole target organ of Tc-99m-NGA. The lack of hepatic uptake of Tc-99m-NGA in healthy chicken corresponded to the lack of the asialoglycoprotein receptors in avian species. Tc-99m-NGA study in a normal volunteer showed apparently different images from those of Tc-99m-pyridoxyl-5-methyltryptophan (PMT) or Tc-99m phytate, suggesting that Tc-99m-NGA is a novel liver imaging radiopharmaceutical. The estimation of the time activity curves in the liver corresponded to the hepatic functional reserves which were evaluated by existing method in 9 clinical cases (2: Normal volunteers, 1: Chronic Active Hepatitis, 1: Liver Cirrhosis (Child A), 2: Liver Cirrhosis (Child B), 3: Liver Cirrhosis (Child C)). The NGA is useful as an imaging agent as well as in the estimation of the hepatic functional reserve, as the dynamic curves of the liver correlates to the asialoglycoprotein receptor concentration.

  8. Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA in rats

    Directory of Open Access Journals (Sweden)

    Maria Kadja Meneses Torres Açucena

    2008-12-01

    Full Text Available This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6 and control (n = 6. After splenectomy (15 days, the administration of 0.1ml of 99mTc-DMSA IV (0.48 MBq was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (pEstudo com objetivo de avaliar se a esplenectomia altera a biodistribuição do 99mTc-DMSA e alguns parâmetros bioquímicos e hematológicos em ratos Wistar. Os animais forma divididos em 2 grupos: esplenectomizados (n=6 e controle(n=6. Após 15 dias, administração de 0,1 ml de 99mTc-DMSA via plexo orbital (0,48 MBq foi realizada. Rim, coração, pulmão, tireóide, estômago, bexiga e fêmur e amostras de sangue foram separadas. Após pesagem e contagem da radioatividade foi determinado o percentual de radioatividade/g (% ATI/g. Dosadas uréia e creatinina sérica, hematócrito, plaquetas e leucócitos. Estatística pelo teste t, significância 0,05 foi realizada. Foi observada redução significante no %ATI/g no rim e sangue (p<0,05 dos animais esplenectomizados, aumento significante (p<0.05 da uréia (88,8±18,6 mg/dL e creatinina (0,56±0,08, comparado aos controles (51,5±1,6; 0,37±0,02mg/dL, respectivamente assim como aumento de leucócitos e plaquetas e redução de hematócrito. Conclui-se que em ratos, a esplenectomia alterou a captação de 99mTc-DMSA pelo rim, e a função renal.

  9. Hyperfixation of Tc-99m ECD in subacute cortical infarction

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jae Seung; Kweon, Sun Uck; Ryu, Jin Sook; Moon, Dae Hyuk; Lee, Hee Kyung [College of Medicine, Ulsan Univ., Seoul (Korea, Republic of)

    2001-07-01

    It has been known that hyperfixation of Tc-99m ECD (HF) is not shown in subacute cerebral infarction because the brain distribution of Tc-99m ECD reflects not only perfusion but also the metabolic status of brain tissue. However, we observed several cases with HF in the subacute pure cortical infarction. To find out the cause of HF in subacute cortical infarction. We assessed the difference in associated cerebral hemodynamics and clinical findings between the subacute cortical infarctions with and without HF. We reviewed 16 patients (63.8{+-}8.6 yr, M/F: 15/1) with pure cortical infarction not involving adjacent subcortical white matter on MRI. All patients underwent acetazolamide stress brain perfusion SPECT using Tc-99m ECD and MRI at subacute period (7.3{+-}4.4 days from ictus). Uptake of Tc-99m ECD in infarcted cortex was assessed visually comparing the contralateral side. To assess the difference in associate clinical findings between the infarctions with and without HF, rCVR of the cerebral territory including infarcted cortex, extent of Gd-enhancement on MRI. Intervals between SPECT and ictus, and the presence of associated ICA stenosis were evaluated. Infarctions were focal (n=8) or multifocal (n=8) and located in frontoparietal cortices on MRI. Twelve patients were accompanied with ipsilateral ICA stenosis. Resting SPECT showed increased cortical uptake (=HF) in 7 patients and decreased in 9. rCVR of the MCA territory was preserved in all of the 7 patients with HF, compared with 4 of the 9 patients without HF (p=0.03). Gd-enhancement was minimal in all of the 7 patients with HF, compared with of the 0 patients without HF (p=0.03). Presence of ipsilateral ICA stenosis and intervals from ictus were not different (p>0.1) Subacute cerebral cortical infarction with HF was more frequently associated with preserved rCVR and minimal destruction of the blood-brain barrier than that without HF. Our findings suggest that HF may result from luxury perfusion of

  10. Technetium 99m pertechnetate scans in congenital hypothyroidism

    Energy Technology Data Exchange (ETDEWEB)

    Bauman, R.A.; Bode, H.H.; Hayek, A.; Crawford, J.D.

    1976-08-01

    Goiters are rarely palpable in infants with congenital hypothyroidism except in the case of maternal ingestion of iodide. The presence or absence of glandular tissue is, however, important for genetic and prognostic counseling and for acceleration of diagnosis in other affected siblings. The detection of thyroid tissue by /sup 99m/Tc pertechnetate scans in a significant number of our patients heretofore considered athyreotic establishes that physical findings and traditional laboratory data are not adequate to determine whether or not thyroid tissue is present.

  11. Imaging of brain tumors and other lesions utilizing /sup 99m/Tc phosphates and /sup 99m/Tc pertechnetate

    Energy Technology Data Exchange (ETDEWEB)

    Schauwecker, D.S.; Burt, R.W.; Richmond, B.D.

    1982-11-01

    The intensity of parenchymal brain lesions was compared using /sup 99m/Tc pertechnetate and /sup 99m/Tc phosphate. The following conclusions were made: 1. If the /sup 99m/Tc phosphate intensity is greater than the intensity of the /sup 99m/Tc pertechnetate scan, and the patient is evaluated within four weeks of ictus, the lesion is a CVA (P less than .001). 2. If the /sup 99m/Tc phosphate intensity is less than or equal to the intensity of the /sup 99m/Tc pertechnetate scan, and the patient is evaluated within four weeks of ictus, the parenchymal lesion is not a CVA (P less than .001). 3. If the evaluation takes place longer than six weeks after ictus, then no evaluation about the nature of the lesion can be made based upon uptake of /sup 99m/Tc phosphate and /sup 99m/Tc pertechnetate.

  12. The single-plasma-sample method for determining the glomerular filtration rate with Tc-99m-diethylenetriamine pentaacetic acid in childhood and adolescence. Is it age-dependent?

    Energy Technology Data Exchange (ETDEWEB)

    Itoh, Kazuo [JR Sapporo General Hospital (Japan); Matsuyama, Takeshi [Tokyo Metropolitan National Health Insurance Organization Fussa Hospital (Japan)

    2002-12-01

    The aim of this study is to assess the accuracy of the pre-existing single-plasma-sample method (SPSM) to measure the glomerular filtration rate (GFR) with Tc-99m-diethylenetriamine pentaacetic acid (Tc-99m-DTPA) in children and adolescents. In addition, the age-independent SPSM is evaluated with two algorithms (Bubeck and Russell) applied for Tc-99m-mercaptoacetyltriglycine (Tc-99m-MAG3) SPSM. The study was performed on 14 patients (12 men and 2 women; age range 3 to 19 yr) with renal diseases. Tc-99m-DTPA (5 MBq/kg) was injected intravenously and thereafter blood samples were taken at 5, 15, 60, 90, 120, 150 and 180 min via the indwelling tube. Radioactivity in the injection syringe and plasma was measured by means of a double-well single-plastic scintillation counter. The true'' GFR as a reference was determined by two methods: 2-exponential curve fitting 7 samples (GFR{sub 7}) and 1-exponential curve fitting 3 samples between 90 and 150 min (GFR{sub 3}) in a slow clearance phase. The GFR{sub 7} and GFR{sub 3} were searched for to the clearance (GFR{sub 1}) estimated from a plasma concentration at various sample times by means of 3 equations designed for children (Groth and Aasted, Ham-I and -II) and 3 for adults (Christensen and Groth, Jacobsson, Itoh). All the SPSM showed close correlations (r>0.95) with the reference methods. Among them, Jacobsson's equation at sample time=120 min tended to be the most accurate (r=0.9826, root mean squared error (RMSE)=7.8 ml/min). On the other hand, Ham-I's equation at sample time=120 min was the most accurate, when it was referred to GFR{sub 3} in correction for overestimation (r=0.9951, RMSE=4.60 ml/min). The Bubeck and Russells' algorithms showed that the regression equation between the GFR{sub 7} and the estimates was different in 2 groups of adults (49 cases) and children/adolescents. Our study indicates that Jacobsson's and Christensen and Groth's equations designed for adults are

  13. Assessment of various parameters in the estimation of differential renal function using technetium-99m mercaptoacetyltriglycine

    Energy Technology Data Exchange (ETDEWEB)

    Lythgoe, M.F.; Gordon, I.; Khader, Z.; Smith, T.; Anderson, P.J. [Department of Radiology, Great Ormond Street Hospital for Children NHS Trust, London (United Kingdom)

    1999-02-01

    Differential renal function (DRF) is an important parameter that should be assessed from virtually every dynamic renogram. With the introduction of technetium-99m mercaptoacetyltriglycine ({sup 99m}Tc-MAG3), a tracer with a high renal extraction, the estimation of DRF might hopefully become accurate and reproducible both between observers in the same institution and also between institutions. The aim of this study was to assess the effect of different parameters on the estimation of DRF. To this end we investigated two groups of children: group A, comprising 35 children with a single kidney (27 of whom had poor renal function), and group B, comprising 20 children with two kidneys and normal global function who also had an associated {sup 99m}Tc-dimercaptosuccinic acid scan ({sup 99m}Tc-DMSA). The variables assessed for their effect on the estimation of DRF were: different operators, the choice of renal regions of interest (ROIs), the applied background subtraction, and six different techniques for analysis of the renogram. The six techniques were based on: linear regression of the slopes in the Rutland-Patlak plot, matrix deconvolution, differential method, integral method, linear regression of the slope of the renograms, and the area under the curve of the renogram. The estimation of DRF was less dependent upon both observer and method in patients with two normally functioning kidneys than in patients with a single kidney. The inter-observer comparison among children in either group was not dependent on either ROI or background subtraction. However, in patients with poor renal function the method of choice for the estimation of DRF was dependent on background subtraction, though not ROI. In children with two kidneys and normal renal function, the estimation of DRF from the 24 techniques gave similar results. Methods that produced DRF values closest to expected results, from either group of children, were the Rutland-Patlak plot and matrix deconvolution methods

  14. Evaluation of usefulness of [sup 99m]Tc-GSA liver scintigraphy in postoperative biliary atresia patients

    Energy Technology Data Exchange (ETDEWEB)

    Kurono, Kenji; Mizutani, Hirokazu; Ohba, Satoru; Hashimoto, Takashi; Yura, Jirou (Nagoya City Univ. (Japan). Faculty of Medicine)

    1994-06-01

    Liver scintigraphy was performed using a newly developed radiopharmaceutical, [sup 99m]Tc-DTPA-galactosyl-human serum albumin ([sup 99m]Tc-GSA), in 17 patients after hepatic portenterostomy for biliary atresia (age: 3 months-14 years old, body weight: 5.8-39 kg). These patients were divided into two groups; good hepatic function group was 3 cases which had no symptom after operation, and poor hepatic function group was 14 cases which needed some therapeutic procedures for ascending cholangitis, GI tract bleeding etc. Dynamic data were obtained by a gamma camera during 20 minutes after intravenous injection of 1.5-3.0 mg (92.5-185 MBq) of [sup 99m]Tc-GSA. The livers were clearly delineated in all cases. We calculated HH[sub 15] (clearance index) for the index of clearance and LHL[sub 15] (receptor index) for the index of hepatic uptake. In good hepatic function group, HH[sub 15] was 0.396[+-]0.059 (mean[+-]S.D.), LHL[sub 15] was 0.960[+-]0.009. In poor hepatic function group, HH[sub 15] was 0.629[+-]0.080, LHL[sub 15] was 0.875[+-]0.060. The difference between the mean value of both indices was statistically significant. Using single photon emission CT, inhomogenisity of liver accumulation of [sup 99m]Tc-GSA was clarified, and focal hepatocyte damage, which could not be detected on CT, was able to be estimated. We consider that [sup 99m]Tc-GSA liver scintigraphy is a useful study for continuous observation of the patients with biliary atresia. (author).

  15. {sup 99m}Tc-sestamibi thyroid uptake in euthyroid individuals and in patients with autoimmune thyroid disease

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Allan O. [Campinas State University, Division of Nuclear Medicine, Department of Radiology, School of Medical Sciences, Campinas (Brazil); Avenida Independencia, Piracicaba, Sao Paulo (Brazil); Zantut-Wittmann, D.E.; Tambascia, M.A. [Campinas State University, Division of Endocrinology, Department of Internal Medicine, School of Medical Sciences, Campinas (Brazil); Nogueira, R.O.; Etchebehere, E.C.S.C.; Lima, M.C.L.; Camargo, E.E.; Ramos, C.D. [Campinas State University, Division of Nuclear Medicine, Department of Radiology, School of Medical Sciences, Campinas (Brazil)

    2005-06-01

    We investigated the biokinetics of {sup 99m}Tc-sestamibi in the thyroid of euthyroid volunteers (EVs) and in patients with autoimmune thyroid diseases and determined the best time interval between {sup 99m}Tc-sestamibi injection and calculation of uptake. Forty EVs, 30 patients with Graves' disease (GD), 15 patients with atrophic Hashimoto's thyroiditis (AHT) and 15 patients with hypertrophic Hashimoto's thyroiditis (HHT) underwent {sup 99m}Tc-sestamibi thyroid scintigraphy. Dynamic images were acquired for 20 min, and static images were obtained 20 min, 60 min and 120 min post injection. Five-, 20-, 60- and 120-min uptake, time to maximal uptake (T{sub max}) and T{sub 1/2} of tracer clearance were calculated. Thyroid hormones and antibodies were measured. {sup 99m}Tc-pertechnetate uptake was investigated in GD patients. T{sub max} was approximately 5 min in all four groups. The mean T{sub 1/2} value for EVs was similar to the GD value and lower than the HHT and AHT values. The mean ({+-}SD) 5-min uptake was 0.13% ({+-}0.05%) for EVs. The 5-min uptake in GD was higher than that in EVs(P<0.001) and correlated with free thyroxine (r=0.54) and with {sup 99m}Tc-pertechnetate uptake (r=0.68). Uptake in HHT was higher than that in AHT (P=0.0003) and EVs (P=0.002). Uptake in AHT was lower than uptake in EVs (P=0.0001). Five minutes is the optimal time interval between {sup 99m}Tc-sestamibi injection and calculation of thyroid uptake. Five-minute uptake differentiates euthyroid individuals from GD patients. There is a high correlation between {sup 99m}Tc-sestamibi and {sup 99m}Tc-pertechnetate uptake in GD. The reduced {sup 99m}Tc-sestamibi uptake in AHT patients is probably due to glandular destruction and fibrosis. Inflammatory infiltrate and high mitochondrial density in thyrocytes possibly explain the increased uptake in GD and HHT. (orig.)

  16. Retention of 99mTc-bicisate in the human brain after intracarotid injection

    DEFF Research Database (Denmark)

    Friberg, L; Andersen, A R; Lassen, N A

    1994-01-01

    flow (rCBF) was measured tomographically with the 133Xe inhalation technique. We applied a three-compartment kinetic model and algorithms modified from a previous analysis of 99mTc d,l-hexamethylpropyleneamine oxide (HM-PAO) kinetics. The bicisate brain uptake and retention curve was very similar...... to that of HM-PAO, and it can be described by a triexponential function including an initial steep component representing the vascular transmitted spike, a second less steep component representing back-diffusion from brain tissue to blood, and a third, very slow component, representing the very slow loss due...

  17. Standardization of (99m)Tc by means of a software coincidence system.

    Science.gov (United States)

    Brito, A B; Koskinas, M F; Litvak, F; Toledo, F; Dias, M S

    2012-09-01

    The procedure followed by the Nuclear Metrology Laboratory, at IPEN, for the primary standardization of (99m)Tc is described. The primary standardization has been accomplished by the coincidence method. The beta channel efficiency was varied by electronic discrimination using a software coincidence counting system. Two windows were selected for the gamma channel: one at 140 keV gamma-ray and the other at 20 keV X-ray total absorption peaks. The experimental extrapolation curves were compared with Monte Carlo simulations by means of code ESQUEMA.

  18. Changes in renal uptake of Tc-99m methylene diphosphonate (MDP) in stone-forming rats

    Energy Technology Data Exchange (ETDEWEB)

    McAfee, J.G.; Thomas, F.D.; Roskopf, M.; Ritter, K.; Lyons, B.; Lilien, O.M.; Schoonmaker, J.E.

    A pyridoxine (vitamin B6)-deficient diet in rats was used as a model of early renal lithiasis to find out if stone-formers could be identified from control animals by differences in the biodistribution of Tc-99m MDP. The mean renal uptake of this agent at 3 hours was about 70% higher in test animals than in controls, but there was considerable overlap between the upper limits of the normal range and lower values in stone-formers. If these results were valid for humans, the metabolic abnormality in males with early stone-forming disease could not be identified with certainty by in vivo measurements of Tc-99m MDP renal uptake alone. However, the skeletal uptake of MDP in the stone-forming animals was depressed by 28 to 35%, compared with control rats. Consequently, the renal to skeletal MDP concentration ratio was invariably elevated in stone-formers beyond the 95 percentile normal range. Unexpectedly, 76% of the pyridoxine-deficient animals had a higher accumulation of MDP in the myocardium than the upper limit of the normal range. The pyridoxine-deficient diet induced no remarkable early changes in the biodistribution or renal clearance of I-131 Hippuran.

  19. Diagnostische Bedeutung von pentavalenter 99m Tc-Dimercaptobern-steinsaure ([V]-DMSA) bei Kopf-Hals-Tumoren

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The purpose of this investigation was to assess the biodistribution of 99m Tc-[V]-DMSA in human being,and its diagnostic value in patients with head and neck tumours.20 patients with histologically confirmed head and neck tumours were examined with planar as well as SPECT scintigraphy. Whole body scintigraphy in different time after injection of 99m Tc-[V]-DM-SA was performed to assess the major sequential organ biodistribution. Our results showed that the blood clearance of 99m Tc-[V]-DMSA was bi-exponential. All organs except kidneys showed a relatively rapid elimination of 99m Tc-[V]-DMSA. The kidneys showed a increasing accumulation in the first 2h, which is probably due to the tubular mabsorption of 99m Tc-[V]-DMSA. In 15 of 20 patients, 19 lesions could be proven by means of planar scintigraphy (corresponding sensitivity of 75%). 29 lesions in 18 patients could however be detected by the application of SPECT (corresponding sensitivity of 90%). Except primary tumours and local lymphadenmetastases in 5 patients distant metastases (3 thorax wall,I liver and I ingvinal/paravesicle) were found. Altogether a sensitivity of 76.9% and a specificity of 71.4% were calculated for the detection of primary tumour. The sensitivity and specificity for the exploration of lymphadenmetastases were 75% and 100%. In conclusion, this study shows that 99m Tc-[V]-DMSA, particularly with SPECT imaging, is useful in localising the primary tumours and lymphadenmetastases as well as distant metastases of head and neck tumours. The possible therapeutic application of 188/186Re-[V]-DMSA is also evaluated.

  20. Pulmonary clearance of 99mTc-DTPA: role of the pulmonary surfactant system.

    NARCIS (Netherlands)

    J.A.H. Bos (Jelle)

    1992-01-01

    textabstractIt is established that in a wide variety of acute and chronic lung diseases the integrity of the alveolar-capillary membrane is altered. Increased permeability of the barrier is thought to cause increased filtration of fluids and proteins into the alveoli and increased absorption of inha

  1. 99mTc: Labeling Chemistry and Labeled Compounds

    Science.gov (United States)

    Alberto, R.; Abram, U.

    This chapter reviews the radiopharmaceutical chemistry of technetium related to the synthesis of perfusion agents and to the labeling of receptor-binding biomolecules. To understand the limitations of technetium chemistry imposed by future application of the complexes in nuclear medicine, an introductory section analyzes the compulsory requirements to be considered when facing the incentive of introducing a novel radiopharmaceutical into the market. Requirements from chemistry, routine application, and market are discussed. In a subsequent section, commercially available 99mTc-based radiopharmaceuticals are treated. It covers the complexes in use for imaging the most important target organs such as heart, brain, or kidney. The commercially available radiopharmaceuticals fulfill the requirements outlined earlier and are discussed with this background. In a following section, the properties and perspectives of the different generations of radiopharmaceuticals are described in a general way, covering characteristics for perfusion agents and for receptor-specific molecules. Technetium chemistry for the synthesis of perfusion agents and the different labeling approaches for target-specific biomolecules are summarized. The review comprises a general introduction to the common approaches currently in use, employing the N x S4-x , [3+1] and 2-hydrazino-nicotinicacid (HYNIC) method as well as more recent strategies such as the carbonyl and the TcN approach. Direct labeling without the need of a bifunctional chelator is briefly reviewed as well. More particularly, recent developments in the labeling of concrete targeting molecules, the second generation of radiopharmaceuticals, is then discussed and prominent examples with antibodies/peptides, neuroreceptor targeting small molecules, myocardial imaging agents, vitamins, thymidine, and complexes relevant to multidrug resistance are given. In addition, a new approach toward peptide drug development is described. The section

  2. Cardiac amyloidosis detection with pyrophosphate-99mTc scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Souza, D.S.F.; Ichiki, W.A.; Coura Filho, G.B.; Izaki, M.; Giorgi, M.C.P.; Soares Junior, J; Meneghetti, J.C. [Universidade de Sao Paulo (FM/USP), SP (Brazil). Fac. de Medicina. Instituto do Coracao. Servico de Medicina Nuclear e Imagem Molecular

    2008-07-01

    Full text: Introduction: Amyloidosis is a rare disease, characterized by extracellular deposition of insoluble amyloid fibrils in organs and tissues. It may affect virtually any system, preferably heart, kidneys and liver. The cardiac involvement produces a spectrum of clinical features, usually with progressive dysfunction. Early diagnosis is important for institution of appropriate therapy. Case report: Male patient, 75 years old, with diagnosed congestive heart failure functional class III and Mobitz II second-degree atrial-ventricular block, was hospitalized for implantation of definitive cardiac pacemaker. Patient mentioned history of worsening effort dyspnoea over a one-month period, progressing to minimum effort, orthopnea, paroxysmal nocturnal dyspnoea and paroxysms of dry cough, and swelling of lower limbs. Echocardiography showed diffuse hypertrophy of left ventricle (LV), with systolic dysfunction due to diffuse hypokinesia and hyperrefringent aspect in the septum. It was questioned a cardiac infiltrating process. Cardiac amyloidosis was considered as a diagnostic hypothesis. The patient underwent a pyrophosphate-{sup 99m}Tc scintigraphy, which showed abnormal tracer uptake in the heart projection, with diffuse pattern on the left ventricle walls, compatible with the clinical suspicion cardiac amyloidosis, which was later confirmed by endomyocardial biopsy. Discussion: In this case report, the patient had clinical and other auxiliary examinations, such as electrocardiography and Doppler echocardiography, compatible with cardiac amyloidosis, which led to implementation with pyrophosphate-{sup 99m}Tc scintigraphy and later endomyocardial biopsy. Cardiac amyloidosis occurs in about half the cases of primary amyloidosis (AL) and is rare in secondary amyloidosis (AA). Its clinical presentation is polymorphic and it can be classified into four distinctive types: restrictive cardiomyopathy, systolic dysfunction, postural hypotension and conduction disorders

  3. Study on 99mTc-MAG3 and 99mTc-DMSA renal accumulation using in vitro cellular model.

    Science.gov (United States)

    Nový, Zbynĕk; Mandíková, Jana; Trejtnar, Frantisek

    2011-02-01

    Mercaptoacetyltriglycine (MAG3) and dimercaptosuccinic acid (DMSA) labelled with technetium-99m belongs to standard renal radiodiagnostics. However, the renal transport mechanisms responsible for their high renal uptake have not been fully explained. In addition, no in vitro experimental study comparing the renal uptake of these radiopharmaceuticals at the cellular level has not been performed. The investigation compared the 99mTc-MAG3 and 99mTc-DMSA renal uptake using primary rat renal cells and evaluated contribution of active and passive transport processes to the renal accumulation. The renal cells were isolated from the rat kidneys by means of the two-phase collagenase perfusion method. The used experimental model showed to be useful tool for such type of investigation. The results documented significant quantitative and qualitative differences in the accumulation of 99mTc-DMSA and 99mTc-MAG3 in the rat isolated cells. The found experimental data indicated several times higher uptake of 99mTc-MAG3 than that found in 99mTc-DMSA. 99mTc-MAG3 cellular uptake was substantially decreased when active, energy-dependent processes were inhibited. However, 99mTc-DMSA accumulation in the renal cells demonstrated only a minor dependency on energy. These findings demonstrate a very different character of the membrane transport determining 99mTc-DMSA and 99mTc-MAG3 renal accumulation.

  4. TOPOLOGY AND VORTEX STRUCTURES OF A CURVING TURBINE CASCADE WITH TIP CLEARANCE ( Ⅱ )- TOPOLOGICAL FLOW PATTERN AND VORTEX STRUCTURE IN THE TRANSVERSE SECTION OF A BLADE CASCADE

    Institute of Scientific and Technical Information of China (English)

    杨庆海; 黄洪雁; 韩万今

    2002-01-01

    By means of ink trace visualization of the flows in conventional straight,positively curved and negatively curved cascades with tip clearance, and measurement of the aerodynamic parameters in transverse section, and by appling topology theory, the topological structures and vortex structure in the transverse section of a blade cascade were analyzed. Compared with conventional straight cascade, blade positive curving eliminates the separation line of the upper passage vortex, and leads the secondary vortex to change from close separation to open separation, while blade negative curving effects merely the positions of singular points and the intensities and scales of vortex.

  5. TOPOLOGY AND VORTEX STRUCTURES OF A CURVING TURBINE CASCADE WITH TIP CLEARANCE ( Ⅰ )- EXPERIMENTAL MODEL AND TOPOLOGICAL FLOW PATTERNS ON BOTH ENDWALLS AND BLADE SURFACES

    Institute of Scientific and Technical Information of China (English)

    杨庆海; 黄洪雁; 韩万今

    2002-01-01

    By means of ink trace visualization of the flows in conventional straight, positively curved and negatively curved cascades with tip clearance, and measurement of the aerodynamic parameters in the transverse section, and by appling topology theory, the structures on both endwalls and blade surfaces were analyzed. Compared with conventional straight cascade, blade positive curving eliminates the separation line of the upper passage vortex and leads the secondary vortex to change from close separation to open separation,while blade negative curving effects merely the positions of singular points and the intensities and scales of vortex.

  6. Technetium-99m-hexamethylpropylene amine oxime lung scintigraphy findings in patients with Behçet's disease.

    Science.gov (United States)

    Gumuser, Gul; Pirildar, Timur; Tarhan, Serdar; Batok, Dilek; Ruksen, Ebru; Sakar, Aysin; Sayit, Elvan

    2011-05-01

    Behçet's disease (BD) is a multisystem disorder that is characterized by vasculitis, and consists of a triad of recurrent ulcers of the oral and genital mucosa with relapsing uveitis. The prevalence of pulmonary involvement varies in the range of 1-10% in various studies, and its complications are severe and life threatening. To objectively assess the degree of pulmonary vascular endothelial damage in BD, the lung uptake, and the clearance rate of technetium-99m-hexamethylpropylene amine oxime (Tc-99m-HMPAO) were determined. Twenty-three nonsmoking patients with BD (10 female, 13 male) and 11 (six female, five male) healthy nonsmoking controls underwent Tc-99m-HMPAO lung scanning, pulmonary function tests (PFT), chest radiograph examination, contrast-enhanced spiral chest computed tomography scanning, and high-resolution computed tomography examinations. Immediately after the bolus injection of 740 MBq of Tc-99m-HMPAO posterior sequential images were obtained at 1-s intervals for 150 s and subsequent images were obtained at one frame per minute (min) over a 10-min period, using a double-headed gamma camera equipped with a low-energy all-purpose parallel hole collimator. Dynamic images were used to calculate the Tc-99m-HMPAO lung clearance rate (T1/2). Simultaneously, a static anterior image, including a large part of the liver, was obtained of 5-min duration at 10 min after the injection. Regular regions of interests were drawn over the midportion of the right lung and the highest activity area of the liver parenchyma was selected and lung/liver (L/Li) uptake ratios were calculated to represent the degree of pulmonary vascular endothelial damage. Chest radiograph, high-resolution computed tomography, and computed tomography findings in patients with BD were nonspecific for BD. There was also a statistically significant difference between patients with BD (30.26 ± 10.55 s) and normal controls (19.53 ± 6.24 s) on their T1/2 values (P = 0.0004). The results show

  7. 99mTechnetium Sestamibi-123Iodine Scintigraphy Is More Accurate Than 99mTechnetium Sestamibi Alone before Surgery for Primary Hyperparathyroidism

    Science.gov (United States)

    Ryhänen, Eeva M.; Schildt, Jukka; Heiskanen, Ilkka; Väisänen, Mika; Ahonen, Aapo; Löyttyniemi, Eliisa; Schalin-Jäntti, Camilla; Välimäki, Matti J.

    2015-01-01

    Objectives. Studies comparing outcome of single-99mTc-methoxyisobutylisonitrile (99mTc-sestamibi) and dual-tracer 99mTc-sestamibi scintigraphy in combination with 123I before primary surgery of primary hyperparathyroidism (PHPT) are scarce. Methods. We compared 99mTc-sestamibi/123I and 99mTc-sestamibi in a single-centre retrospective series of 269 PHPT patients. The results were related to laboratory, surgical and histological findings. Results. 99mTc-sestamibi/123I and 99mTc-sestamibi were positive in 206 (76.6%) and 111 (41.3%) of 269 patients, respectively (P < 0.001). Accuracies for 99mTc-sestamibi/123I and 99mTc-sestamibi were 63.4% and 34.9%, respectively (96% CI, P < 0.001). Prevalence of multiglandular disease was 15.2%. In multiglandular disease, 99mTc-sestamibi/123I and 99mTc-sestamibi revealed 43.8 and 22.1% of pathological glands, respectively (P < 0.001). Cure rate was similar for patients with (191/206; 92.7%) and without (59 of 63; 93.7%) a positive 99mTc-sestamibi/123I finding. Duration of targeted surgery (one or two quadrants) was 21 and 15 minutes shorter than bilateral neck exploration, respectively (both P < 0.001). Higher serum calcium (P = 0.014) and PTH (P = 0.055) concentrations and larger tumours (P < 0.001) characterized the 206 patients with a positive preoperative scan who were cured by removal of a single adenoma. Conclusions. 99mTc-sestamibi/123I scintigraphy is more accurate than 99mTc-sestamibi before surgery of PHPT. However, outcome of surgery is not determined by scintigraphy alone. PMID:25722888

  8. (99m)Technetium Sestamibi-(123)Iodine Scintigraphy Is More Accurate Than (99m)Technetium Sestamibi Alone before Surgery for Primary Hyperparathyroidism.

    Science.gov (United States)

    Ryhänen, Eeva M; Schildt, Jukka; Heiskanen, Ilkka; Väisänen, Mika; Ahonen, Aapo; Löyttyniemi, Eliisa; Schalin-Jäntti, Camilla; Välimäki, Matti J

    2015-01-01

    Objectives. Studies comparing outcome of single-(99m)Tc-methoxyisobutylisonitrile ((99m)Tc-sestamibi) and dual-tracer (99m)Tc-sestamibi scintigraphy in combination with (123)I before primary surgery of primary hyperparathyroidism (PHPT) are scarce. Methods. We compared (99m)Tc-sestamibi/(123)I and (99m)Tc-sestamibi in a single-centre retrospective series of 269 PHPT patients. The results were related to laboratory, surgical and histological findings. Results. (99m)Tc-sestamibi/(123)I and (99m)Tc-sestamibi were positive in 206 (76.6%) and 111 (41.3%) of 269 patients, respectively (P < 0.001). Accuracies for (99m)Tc-sestamibi/(123)I and (99m)Tc-sestamibi were 63.4% and 34.9%, respectively (96% CI, P < 0.001). Prevalence of multiglandular disease was 15.2%. In multiglandular disease, (99m)Tc-sestamibi/(123)I and (99m)Tc-sestamibi revealed 43.8 and 22.1% of pathological glands, respectively (P < 0.001). Cure rate was similar for patients with (191/206; 92.7%) and without (59 of 63; 93.7%) a positive (99m)Tc-sestamibi/(123)I finding. Duration of targeted surgery (one or two quadrants) was 21 and 15 minutes shorter than bilateral neck exploration, respectively (both P < 0.001). Higher serum calcium (P = 0.014) and PTH (P = 0.055) concentrations and larger tumours (P < 0.001) characterized the 206 patients with a positive preoperative scan who were cured by removal of a single adenoma. Conclusions. (99m)Tc-sestamibi/(123)I scintigraphy is more accurate than (99m)Tc-sestamibi before surgery of PHPT. However, outcome of surgery is not determined by scintigraphy alone.

  9. Biodistribution of {sup 99m}Tc-lactosylated serum albumin in mice with diethylnitrosamine or thiacetamide induced liver injury

    Energy Technology Data Exchange (ETDEWEB)

    Whang, Jae Seok; Ahn, Byeong Cheol; Sung, Young Ok; Seo, Ji Hyoung; Bae, Jin Ho; Jeong, Shin Young; Yoo, Jung Soo; Lee, Jae Tae; Lee, Kyu Bo [Kyungpook National University Medical School, Daegu (Korea, Republic of); Jeong, Jae Min [Seoul National University College of Medicine, Seoul (Korea, Republic of)

    2005-02-15

    Tc-99m labeled diethylenetriaminepentaacetic acid (DTPA)-coupled galactosylated human serum albumin (GSA) is a currently used imaging agent for asialoglycoprotein receptor (ASGPR) of the liver, but it has several shortcomings. Recently a new ASGPR imaging agent, {sup 99m}Tc-lactosylated human serum albumin (LSA), with simple labeling procedure, high labeling efficiency, high stability was developed. In order to assess the feasibility of the {sup 99m}Tc-LSA as a ASGPR imaging radiopharmaceuticals, we performed biodistribution study of the tracer in liver injured mice model and the results were compared with histologic data. To induce hepatic damage in ICR mice, diethylnitrosamine (DEN) (60 mg/kg/week X 5 time, low dose or 180 mg/kg/week X 2 time, high dose) and thioacetamide (TAA) (50 mg/kg X 1 time) were administrated intraperitoneally. Degree of liver damage was evaluated by tissue hematoxilin-eosin stain, and expression of asialoglycoprotein receptor (ASGPR) was assessed by immunohistochemistry using ASGPR antibody. {sup 99m}Tc-LSA was intravenously administrated via tail vein in DEN or TAA treated mice, and biodistribution study of the tracer was also performed. DEN treated mice showed ballooning of hepatocyte and inflammatory cell infiltration in low dose group and severe hepatocyte necrosis in high dose group, and low dose group showed higher ASGPR staining than control mice in immunohistochemical staining. TAA treated mice showed severe hepatic necrosis. {sup 99m}Tc-LSA biodistribution study showed that mice with hepatic necrosis induced by high dose. DEN or TAA revealed higher blood activity and lower liver activity than control mice, due to slow clearance of the tracer by the liver. The degree of liver uptake was inversely correlated with the degree of histologic liver damage. But low dose DEN treated mice with mild hepatic injury showed normal blood clearance and hepatic activity, partly due to overexpression of ASGPR in mice with mild degree hepatic

  10. Comparative evaluation of glutamate-sensitive radiopharmaceuticals: Technetium-99m-glutamic acid and technetium-99m-diethylenetriaminepentaacetic acid-bis(glutamate) conjugate for tumor imaging.

    Science.gov (United States)

    Kakkar, Dipti; Tiwari, Anjani K; Chuttani, Krishna; Kaul, Ankur; Singh, Harpal; Mishra, Anil K

    2010-12-01

    Single-photon emission computed tomography has become a significant imaging modality with huge potential to visualize and provide information of anatomic dysfunctions that are predictive of future diseases. This imaging tool is complimented by radiopharmaceuticals/radiosubstrates that help in imaging specific physiological aspects of the human body. The present study was undertaken to explore the utility of technetium-99m (⁹⁹(m)Tc)-labeled glutamate conjugates for tumor scintigraphy. As part of our efforts to further utilize the application of chelating agents, glutamic acid was conjugated with a multidentate ligand, diethylenetriaminepentaacetic acid (DTPA). The DTPA-glutamate conjugate [DTPA-bis(Glu)] was well characterized by IR, NMR, and mass spectroscopy. The biological activity of glutamic acid was compared with its DTPA conjugate by radiocomplexation with ⁹⁹(m)Tc (labeling efficiency ≥98%). In vivo studies of both the radiolabeled complexes ⁹⁹(m)Tc-Glu and ⁹⁹(m)Tc-DTPA-bis(Glu) were then carried out, followed by gamma scintigraphy in New Zealand albino rabbits. Improved serum stability of ⁹⁹(m)Tc-labeled DTPA conjugate indicated that ⁹⁹(m)Tc remained bound to the conjugate up to 24 hours. Blood clearance showed a relatively slow washout of the DTPA conjugate when compared with the labeled glutamate. Biodistribution characteristics of the conjugate in Balb/c mice revealed that DTPA conjugation of glutamic acid favors less accumulation in the liver and bone and rapid renal clearance. Tumor scintigraphy in mice showed increasing tumor accumulation, stable up to 4 hours. These preliminary studies show that ⁹⁹(m)Tc-DTPA-bis(Glu) can be a useful radiopharmaceutical for diagnostic applications in single-photon emission computed tomography imaging.

  11. Spatially resolved assessment of hepatic function using 99mTc-IDA SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Hesheng [Department of Radiation Oncology, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, Michigan 48109 (United States); Cao, Yue [Department of Radiation Oncology, Radiology, and Biomedical Engineering, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, Michigan 48109 (United States)

    2013-09-15

    Purpose: 99mTc-iminodiacetic acid (IDA) hepatobiliary imaging is usually quantified for hepatic function on the entire liver or regions of interest (ROIs) in the liver. The authors presented a method to estimate the hepatic extraction fraction (HEF) voxel-by-voxel from single-photon emission computed tomography (SPECT)/CT with a 99mTc-labeled IDA agent of mebrofenin and evaluated the spatially resolved HEF measurements with an independent physiological measurement.Methods: Fourteen patients with intrahepatic cancers were treated with radiation therapy (RT) and imaged by 99mTc-mebrofenin SPECT before and 1 month after RT. The dynamic SPECT volumes were with a resolution of 3.9 × 3.9 × 2.5 mm{sup 3}. Throughout the whole liver with approximate 50 000 voxels, voxelwise HEF quantifications were estimated and compared between using arterial input function (AIF) from the heart and using vascular input function (VIF) from the spleen. The correlation between mean of the HEFs over the nontumor liver tissue and the overall liver function measured by Indocyanine green clearance half-time (T1/2) was assessed. Variation of the voxelwise estimation was evaluated in ROIs drawn in relatively homogeneous regions of the livers. The authors also examined effects of the time range parameter on the voxelwise HEF quantification.Results: Mean of the HEFs over the liver estimated using AIF significantly correlated with the physiological measurement T1/2 (r= 0.52, p= 0.0004), and the correlation was greatly improved by using VIF (r= 0.79, p < 0.0001). The parameter of time range for the retention phase did not lead to a significant difference in the means of the HEFs in the ROIs. Using VIF and a retention phase time range of 7–30 min, the relative variation of the voxelwise HEF in the ROIs was 10%± 6% of respective mean HEF.Conclusions: The voxelwise HEF derived from 99mTc-IDA SPECT by the deconvolution analysis is feasible to assess the spatial distribution of hepatic function in the

  12. Urinary bladder blood flow. I. Comparison of clearance of locally injected 99mtechnetium pertechnate and radioactive microsphere technique in dogs

    DEFF Research Database (Denmark)

    Krøyer, Kristian; Bülow, J; Nielsen, S L

    1990-01-01

    The blood flow of the dog urinary bladder measured by radioactive microsphere technique was compared to the clearance of locally injected 99mTechnetium pertechnate (99mTc) in the bladder wall. In semilogarithmic plots the 99mTc washout curves showed a multiexponential course. From the initial...... slopes (median 5.7 min) the bladder blood flow was calculated to be only 30-62% of the results obtained from the radioactive microsphere technique (blood flow in the muscular layer 21.7-44.8 ml/100 g/min). These lower values imply that the rate of removal of the hydrophilic tracer 99mTc at these flow...

  13. Synthesis and preliminary biological evaluation of the first (99m)Tc(I)-specific semi-rigid tridentate ligand based on a click chemistry strategy.

    Science.gov (United States)

    Guizani, Sihem; Malek Saied, Nadia; Picard, Claude; Benoist, Eric; Saidi, Mouldi

    2014-03-01

    A novel bifunctional chelating agent based on a click chemistry strategy has been synthesized and characterized on the basis of spectroscopic techniques. The metal chelating part of this new class of tridentate N2O ligand combined a triazole unit and an aromatic ring. This latter semi-rigid framework induced a pre-organization of the chelating cavity, improving the stability of the corresponding metallic complexes (M = (99m) Tc, Re). Thus, the (99m) Tc(CO)3 complex, obtained with good yield and excellent radiochemical purity (>90%), exhibited a high in vitro serum stability. Tissue biodistribution in normal mice showed a rapid clearance, no long-term retention in organs and no in vivo reoxidation of technetium-99m, making this compound a promising (99m)Tc-chelating system.

  14. Different uptake of 99mTc-ECD adn 99mTc-HMPAO in the same brains: analysis by statistical parametric mapping.

    Science.gov (United States)

    Hyun, Y; Lee, J S; Rha, J H; Lee, I K; Ha, C K; Lee, D S

    2001-02-01

    The purpose of this study was to investigate the differences between technetium-99m ethyl cysteinate dimer (99mTc-ECD) and technetium-99m hexamethylpropylene amine oxime (99mTc-HMPAO) uptake in the same brains by means of statistical parametric mapping (SPM) analysis. We examined 20 patients (9 male, 11 female, mean age 62+/-12 years) using 99mTc-ECD and 99mTc-HMPAO single-photon emission tomography (SPET) and magnetic resonance imaging (MRI) of the brain less than 7 days after onset of stroke. MRI showed no cortical infarctions. Infarctions in the pons (6 patients) and medulla (1), ischaemic periventricular white matter lesions (13) and lacunar infarction (7) were found on MRI. Split-dose and sequential SPET techniques were used for 99mTc-ECD and 99mTc-HMPAO brain SPET, without repositioning of the patient. All of the SPET images were spatially transformed to standard space, smoothed and globally normalized. The differences between the 99mTc-ECD and 99mTc-HMPAO SPET images were statistically analysed using statistical parametric mapping (SPM) 96 software. The difference between two groups was considered significant at a threshold of uncorrected P values less than 0.01. Visual analysis showed no hypoperfused areas on either 99mTc-ECD or 99mTc-HMPAO SPET images. SPM analysis revealed significantly different uptake of 99mTc-ECD and 99mTc-HMPAO in the same brains. On the 99mTc-ECD SPET images, relatively higher uptake was observed in the frontal, parietal and occipital lobes, in the left superior temporal lobe and in the superior region of the cerebellum. On the 99mTc-HMPAO SPET images, relatively higher uptake was observed in the medial temporal lobes, thalami, periventricular white matter and brain stem. These differences in uptake of the two tracers in the same brains on SPM analysis suggest that interpretation of cerebral perfusion is possible using SPET with 99mTc-ECD and 99mTc-HMPAO.

  15. Different uptake of {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO in the same brains: analysis by statistical parametric mapping

    Energy Technology Data Exchange (ETDEWEB)

    Hyun, I.Y. [Dept. of Nuclear Medicine, Inha University College of Medicine, Incheon (Korea); Lee, J.S.; Lee, D.S. [Dept. of Nuclear Medicine, Seoul National University College of Medicine, Seoul (Korea); Rha, J.H.; Lee, I.K.; Ha, C.K. [Dept. of Neurology, Inha University College of Medicine, Incheon (Korea)

    2001-02-01

    The purpose of this study was to investigate the differences between technetium-99m ethyl cysteinate dimer ({sup 99m}Tc-ECD) and technetium-99m hexamethylpropylene amine oxime ({sup 99m}Tc-HMPAO) uptake in the same brains by means of statistical parametric mapping (SPM) analysis. We examined 20 patients (9 male, 11 female, mean age 62{+-}12 years) using {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO single-photon emission tomography (SPET) and magnetic resonance imaging (MRI) of the brain less than 7 days after onset of stroke. MRI showed no cortical infarctions. Infarctions in the pons (6 patients) and medulla (1), ischaemic periventricular white matter lesions (13) and lacunar infarction (7) were found on MRI. Split-dose and sequential SPET techniques were used for {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO brain SPET, without repositioning of the patient. All of the SPET images were spatially transformed to standard space, smoothed and globally normalized. The differences between the {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO SPET images were statistically analysed using statistical parametric mapping (SPM) 96 software. The difference between two groups was considered significant at a threshold of uncorrected P values less than 0.01. Visual analysis showed no hypoperfused areas on either {sup 99m}Tc-ECD or {sup 99m}Tc-HMPAO SPET images. SPM analysis revealed significantly different uptake of {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO in the same brains. On the {sup 99m}Tc-ECD SPET images, relatively higher uptake was observed in the frontal, parietal and occipital lobes, in the left superior temporal lobe and in the superior region of the cerebellum. On the {sup 99m}Tc-HMPAO SPET images, relatively higher uptake was observed in the medial temporal lobes, thalami, periventricular white matter and brain stem. These differences in uptake of the two tracers in the same brains on SPM analysis suggest that interpretation of cerebral perfusion is possible using SPET with {sup 99m}Tc-ECD and

  16. 99mTc-octreotide Receptor Scintigraphy in NCI-H446 Small Cell Lung Cancer Nude Mice Model

    Directory of Open Access Journals (Sweden)

    Chao LI

    2015-01-01

    Full Text Available Background and objective For highly aggressive small cell lung cancer (SCLC, early diagnosis is important for its prognosis, but the current inspection methods are more limited, with poor specificity of the traditional imaging methods, and the high cost of PET/CT, difficult to popularization and application. SCLC is kind of neuroendocrine tumors, high expression of somatostatin receptors, which is the cornerstone of its early molecular imaging diagnosis. The aim of this study is to observe the biodistribution and metabolism of 99mTc-octreotide in normal and the human SCLC bearing nude mice. Methods Dynamic and static scintigraphy at 0.5 h, 2 h, 3 h, 4 h were performed in both normal and tumor bearing nude mice after intravenous injection of 99mTc-octreotide. The technique of drawing region of interest (ROI was used to obtain the averaged pixel counts and the activity-time (A-T curve of brain, heart, lung, liver, kidney, tumor, respectively. Results ① The biodistribution study in normal nude mice showed highest uptake in kidney and liver, lower in lung and heart, lowest in brain. Most 99mTc-octreotide was excreted via kidney. ② All tumors were displayed clearly at 3 h postinjection of 99mTc-octreotide. The averaged T/N ratio at 0.5 h, 2 h, 3 h, 4 h postinjection of 99mTc-octreotide was 1.163±0.03, 2.08±0.12, 3.03±0.23, 2.689±0.31, respectively (F=51.69, P<0.000,1. The radioactivity of tumor was lower than liver, and similar with the lung. The curve of tumor showed a radioactivity peak at 2 min-3 min postinjection. Conclusion 99mTc-octreotide receptor imaging on nude mice bearing SCLC shares high positive rate, especially at 3 h postinjection.

  17. [99mTc-octreotide receptor scintigraphy in NCI-H446 small cell lung cancer nude mice model].

    Science.gov (United States)

    Li, Chao; Zuo, Shuyao; Wang, Xufu; Liu, Xinfeng; Wang, Guoming; Wu, Fengyu

    2015-01-01

    For highly aggressive small cell lung cancer (SCLC), early diagnosis is important for its prognosis, but the current inspection methods are more limited, with poor specificity of the traditional imaging methods, and the high cost of PET/CT, difficult to popularization and application. SCLC is kind of neuroendocrine tumors, high expression of somatostatin receptors, which is the cornerstone of its early molecular imaging diagnosis. The aim of this study is to observe the biodistribution and metabolism of 99mTc-octreotide in normal and the human SCLC bearing nude mice. Dynamic and static scintigraphy at 0.5 h, 2 h, 3 h, 4 h were performed in both normal and tumor bearing nude mice after intravenous injection of 99mTc-octreotide. The technique of drawing region of interest (ROI) was used to obtain the averaged pixel counts and the activity-time (A-T) curve of brain, heart, lung, liver, kidney, tumor, respectively. ① The biodistribution study in normal nude mice showed highest uptake in kidney and liver, lower in lung and heart, lowest in brain. Most 99mTc-octreotide was excreted via kidney. ② All tumors were displayed clearly at 3 h postinjection of 99mTc-octreotide. The averaged T/N ratio at 0.5 h, 2 h, 3 h, 4 h postinjection of 99mTc-octreotide was 1.163 ± 0.03, 2.08 ± 0.12, 3.03 ± 0.23, 2.689 ± 0.31, respectively (F=51.69, Plung. The curve of tumor showed a radioactivity peak at 2 min-3 min postinjection. 99mTc-octreotide receptor imaging on nude mice bearing SCLC shares high positive rate, especially at 3 h postinjection.

  18. Comparison of the Efficiency for Tc-99m Tin-colloid and Tc-99m Phytate in Sentinel Node Detection in Breast Cancer Patients

    Energy Technology Data Exchange (ETDEWEB)

    Seok, Ju Won [Chung-Ang University, Seoul (Korea, Republic of); Kim, In Ju [College of Medicine, Pusan National University, Pusan (Korea, Republic of)

    2008-12-15

    Lymphoscintigraphy and sentinel node biopsy has become a standard method for detection of axillary lymph node metastasis in breast cancer patients, but the standard radiopharmaceutical was not prepared. About detection of axillary lymph node metastasis by lymphoscintigraphy and sentinel node biopsy in breast cancer patient, we compared the results of Tc-99m Tin-colloid and Tc-99m Phytate by subareolar injection. This study included 382 breast cancer patients who were performed operation during 2001-2008. Three hundred forty nine patients was injected 0.8 ml of Tc-99m Tin-colloid (37-185 MBq) by subareolar injection. Thirty three patients was injected 0.8 ml of Tc-99m Phytate (37-185 MBq). Lymphoscintigraphy was performed in supine position and sentinel node localization was performed by hand-held gamma probe in operation. Among 349 patients by Tc-99m Tin-colloid, 312 cases (89.4%) localized the sentinel node by lymphoscintigraphy, 304 cases (87.1%) localized by gamma probe. Among 33 patients by Tc-99m Phytate, 32 cases (97.0%) localized by lymphoscintigraphy, 33 cases (100%) localized by gamma probe. Detection rate by lymphoscintigraphy and gamma probe was superior for Tc-99m Phytate compared to that with Tc-99m Tin-colloid, with a statistically significant difference. (p<0.05, p<0.05) Tc-99m Phytate is a better choice for localization of sentinel node than Tc-99m Tin-colloid in breast cancer patients.

  19. Comparative biological evaluation between {sup 99m}Tc tricarbonyl and {sup 99m}Tc-Sn(II) levosalbutamol as a β{sub 2}-adrenoceptor agonist

    Energy Technology Data Exchange (ETDEWEB)

    Sanad, Mahmoud H. [Atomic Energy Authority, Cairo (Egypt). Labeled Compounds Dept.; Borai, Emad H. [Atomic Energy Authority, Cairo (Egypt). Analytical Chemistry Dept.

    2015-07-01

    This study describes the comparison between {sup 99m}Tc-tricarbonyl and {sup 99m}Tc-Sn (II) levosalbutamol as a β{sub 2}-adrenoceptors radiopharmaceutical and evaluation of their different biological characteristics using experimental animals. Levosalbutamol was labeled firstly with {sup 99m}Tc in the presence of SnCl{sub 2} . 2H{sub 2}O as a reducing agent under the optimum conditions: pH 8, 50 μg SnCl{sub 2} . 2H{sub 2}O, room temperature, 40 μg levosalbutamol and 30 min reaction time to give a maximum radiochemical yield of 98 ± 0.1%. The obtained {sup 99m}Tc-levosalbutamol was stable for a time up to 8 h. Secondly, {sup 99m}Tc-tricarbonyl ([{sup 99m}Tc(CO){sub 3}(H{sub 2}O){sub 3}]{sup +}) levosalbutamol was prepare under 30 min heating at 100 C. Labeling yield and stability were analyzed by high performance liquid chromatography (labeling yield >99% and stability for 8 h). Biodistribution investigation showed that, the maximum uptake ratio of the {sup 99m}Tc-levosalbutamol ({sup 99m}Tc-Lev) between lung and heart was 2.34 ± 0.62 % of the injected activity/g tissue organ, at 30 min post-injection. But in case of {sup 99m}Tc-tricarbonyl levosalbutamol ({sup 99m}Tc-tricarbonyl Lev) the maximum uptake ratio was 3.6 ± 0.11 of the injected activity/g tissue organ, at 30 min post-injection. This indicates that {sup 99m}Tc-tricarbonyl levosalbutamol was more selective for lung β{sub 2}-adrenoceptors than {sup 99m}Tc-levosalbutamol. These results introduce {sup 99m}Tc-tricarbonyl levosalbutamol as a novel potential radiopharmaceutical for lung imaging.

  20. A novel ternary ligand system useful for preparation of cationic (99m)Tc-diazenido complexes and (99m)Tc-labeling of small biomolecules.

    Science.gov (United States)

    Kim, Young-Seung; He, Zhengjie; Hsieh, Wen-Yuan; Liu, Shuang

    2006-01-01

    This report describes a novel ternary ligand system composed of a phenylhydrazine, a crown ether-containing dithiocarbamate (DTC), and a PNP-type bisphosphine (PNP). The combination of three different ligands with (99m)Tc results in cationic (99m)Tc-diazenido complexes, [(99m)Tc(NNAr)(DTC)(PNP)]+, with potential radiopharmaceuticals for heart imaging. Synthesis of cationic (99m)Tc-diazenido complexes can be accomplished in two steps. For example, the reaction of phenylhydrazine with (99m)TcO4- at 100 degrees C in the presence of excess stannous chloride and 1,2-diaminopropane-N,N,N',N'-tetraacetic acid (PDTA) results in the [(99m)Tc(NNPh)(PDTA)n] intermediate, which then reacts with sodium N-(dithiocarbamato)-2-aminomethyl-15-Crown-5 (L4) and N,N-bis[2-(bis(3-ethoxypropyl)phosphino)ethyl]ethoxyethylamine (PNP6) at 100 degrees C for 15 min to give the complex, [(99m)Tc(NNPh)(L4)(PNP6)]+ in high yield (>90%). Cationic complexes [(99m)Tc(NNPh)(DTC)(PNP)]+ are stable for > or = 6 h. Their composition was determined to be 1:1:1:1 for Tc:NNPh:DTC:PNP using the mixed-ligand experiments on the tracer ((99m)Tc) level and was further confirmed by the ESI-MS spectral data of a model compound [Re(NNPh)(L4)(L6)]+. It was found that both DTCs and bisphosphines have a significant impact on the lipophilicity of their cationic (99m)Tc-diazenido complexes. Results from a (99m)Tc-labeling efficiency experiment showed that 4-hydrazinobenzoic acid (HYBA) might be useful as a bifunctional coupling agent for (99m)Tc-labeling of small biomolecules. However, the (99m)Tc-labeling efficiency of HYBA is much lower than that of 6-hydrazinonicotinic acid (HYNIC) with tricine and trisodium triphenylphosphine-3,3',3''-trisulfonate (TPPTS) as coligands.

  1. {sup 99m}Tc-HYNIC-derivatized ternary ligand complexes for {sup 99m}Tc-labeled polypeptides with low in vivo protein binding

    Energy Technology Data Exchange (ETDEWEB)

    Ono, Masahiro; Arano, Yasushi E-mail: arano@p.chiba-u.ac.jp; Mukai, Takahiro; Fujioka, Yasushi; Ogawa, Kazuma; Uehara, Tomoya; Saga, Tsuneo; Konishi, Junji; Saji, Hideo

    2001-04-01

    6-Hydrazinopyridine-3-carboxylic acid (HYNIC) is a representative agent used to prepare technetium-99m ({sup 99m}Tc)-labeled polypeptides with tricine as a coligand. However, {sup 99m}Tc-HYNIC-labeled polypeptides show delayed elimination rates of the radioactivity not only from the blood but also from nontarget tissues such as the liver and kidney. In this study, a preformed chelate of tetrafluorophenol (TFP) active ester of [{sup 99m}Tc](HYNIC)(tricine)(benzoylpyridine: BP) ternary complex was synthesized to prepare {sup 99m}Tc-labeled polypeptides with higher stability against exchange reactions with proteins in plasma and lysosomes using the Fab fragment of a monoclonal antibody and galactosyl-neoglycoalbumin (NGA) as model polypeptides. When incubated in plasma, [{sup 99m}Tc](HYNIC-Fab)(tricine)(BP) showed significant reduction of the radioactivity in high molecular weight fractions compared with [{sup 99m}Tc](HYNIC-Fab)(tricine){sub 2.} When injected into mice, [{sup 99m}Tc](HYNIC-NGA)(tricine)(BP) was metabolized to [{sup 99m}Tc](HYNIC-lysine)(tricine)(BP) in the liver with no radioactivity detected in protein-bound fractions in contrast to the observations with [{sup 99m}Tc](HYNIC-NGA)(tricine){sub 2.} In addition, [{sup 99m}Tc](HYNIC-NGA)(tricine)(BP) showed significantly faster elimination rates of the radioactivity from the liver as compared with [{sup 99m}Tc](HYNIC-NGA)(tricine){sub 2.} Similar results were observed with {sup 99m}Tc-labeled Fab fragments where [{sup 99m}Tc](HYNIC-Fab)(tricine)(BP) exhibited significantly faster elimination rates of the radioactivity not only from the blood but also from the kidney. These findings indicated that conjugation of [{sup 99m}Tc](HYNIC)(tricine)(BP) ternary ligand complex to polypeptides accelerated elimination rates of the radioactivity from the blood and nontarget tissues due to low binding of the [{sup 99m}Tc](HYNIC)(tricine)(BP) complex with proteins in the blood and in the lysosomes. Such characteristics

  2. Stabilization of Tc-99m radiopharmaceuticals by chemical additives (NOTE

    Directory of Open Access Journals (Sweden)

    NADEZDA VUKICEVIC

    2001-09-01

    Full Text Available The reliability and applicability of the preparation of the three, for nuclear medicine very important, 99mTc-radiopharmaceuticals from the inactive (technetium-cold kit solutions were tested. Each examined commercial kit was dissolved in saline (0.9 % NaCl. The conditions of the storage of the inactive kit solutions till labeling were examined. The main problem is the stablity of the reductant stannous ions which is very difficult to predict. To stabilize and ensure a good quality of the labeled radiopharmaceuticals, ascorbic or gentisic acid were added. It was found that the best results were obtained by keeping the samples frozen at –20 ?C. Both stabilizers can be used but for an effective protection much lower concentrations of ascorbic acid are needed. Its concentrations of 12–60 mg/ml of the kit, stabilized dimercaptosuccinate (DMS and pyrophosphate (PyP for about 7–8 days. The solution of 2,3-dicarboxypropane-1,1-diphosphonate (DPD was found to be stable even without the stabilizer. This could be attributed probably to the chemical nature of this complex. However, in routine praxis the applied procedure demands great care and personel very experienced in radiopharmacy.

  3. The retention mechanism of technetium-99m-HM-PAO

    DEFF Research Database (Denmark)

    Neirinckx, R D; Burke, J F; Harrison, R C

    1988-01-01

    Preparations of d,l- and meso-hexamethylpropyleneamine oxime (HM-PAO) labeled with technetium-99m were added to rat brain homogenates diluted with phosphate buffer (1:10). The conversion of d,l-HM-PAO to hydrophilic forms took place with an initial rate constant of 0.12 min-1. Incubation...... of the brain homogenate with 2% diethyl maleate for 5 h decreased the homogenate's measured glutathione (GSH) concentration from 160 to 16 microM and decreased the conversion rate to 0.012 min-1. Buffered aqueous solutions of glutathione rapidly converted the HM-PAO tracers to hydrophilic forms having the same...... chromatographic characteristics as found in the brain homogenates. The rate constant for the conversion reaction of d,l-HM-PAO in GSH aqueous solution was 208 and 317 L/mol/min in two different assay systems and for meso-HM-PAO the values were 14.7 and 23.2 L/mol/min, respectively. Rat brain has a GSH...

  4. Preoperative radiological diagnosis by {sup 99m}Tc{center_dot}MIBI-{sup 99m}Tc subtraction scintigraphy for primary hyperparathyroidism

    Energy Technology Data Exchange (ETDEWEB)

    Inouye, Takahiro; Tomita, Toshiki; Shinden, Seiichi; Takagi, Hitoshi [Tochigi Prefecture Saiseikai Utsunomiya Hospital (Japan); Kano, Shigeru

    1996-04-01

    Preoperative radiological diagnosis constitutes the most important factor for the surgical treatment of hyperparathyroidism. In this regard, MRI is useful for detecting the abnormal parathyroid, but it is often difficult to localize it using MRI only. It is thus necessary to combine this procedure with excellent subtraction scintigraphy. We performed both {sup 201}Tl-{sup 99m}Tc and {sup 99m}Tc{center_dot}MIBI-{sup 99m}Tc subtraction scintigraphy in seven patients with primary hyperparathyroidism and compared them the radiological results. Five patients presented parathyroid adenomas and the rest hypertrophy of the parathyroid. We could detect the abnormal parathyroid in four patients (57.1%) by {sup 201}Tl-{sup 99m}Tc subtraction scintigraphy and in six patients (85.7%) by {sup 99m}Tc{center_dot}MIBI-{sup 99m}Tc subtraction scintigraphy. We therefore believe that {sup 99m}Tc{center_dot}MIBI-{sup 99m}Tc subtraction scintigraphy will become an essential examination for primary hyperparathyroidism rather than the presently employed {sup 201}Tl-{sup 99m}Tc subtraction scintigraphy. (author).

  5. /sup 99m/Tc-aminohexylidendiphosphonate and /sup 99m/Tc-pyrophosphate in the scintigraphic diagnosis of experimental myocardial infarction in dogs

    Energy Technology Data Exchange (ETDEWEB)

    Duska, F.; Hadas, L.; Vizda, J.; Kafka, P.; Urbanova, E.; Palicka, V.; Mazurova, Y.; Kuba, M.; Grossman, V.

    1988-10-01

    /sup 99m/Tc-aminohexylidendiphosphonate (/sup 99m/Tc-AHDP) is a new Czechoslovak pharmaceutical of the phosphonate line which contains the amino group NH/sub 2/ in its molecule. This substance was tested in 5 dogs with experimentally provoked 48-h old myocardial infarction. The in-vivo scan and the radioactivity of tissue samples demonstrated that /sup 99m/Tc-AHDP is as suitable for imaging acute myocardial infarction as is the commonly used /sup 99m/Tc-pyrophosphate.

  6. Analysis of sup 99m Tc-labeled radiopharmaceuticals by high-performance liquid chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Muto, Toshio (Tokyo Metropolitan Isotope Research Center (Japan))

    1990-02-01

    High-performance liquid chromatography (HPLC) equiped with on line radiometric and optical detectors (i.e. radio-HPLC) have been applied to the radiochemical analysis of commonly-used {sup 99m}Tc-radio pharma ceuticals with a view point to check the radiochemical purities of the compounds. Chromatographic conditions were determined by examination of the types of column, mobile phase and pH. An aqueous size-exclusion (Shim-pack Diol-300) and reversed-phase column (Zorbax-ODS) were found to be suitable for {sup 99m}Tc-HSA and the other {sup 99m}Tc-agents, respectively. The analysis of low molecular weight {sup 99m}Tc-agents (e.g. {sup 99m}Tc-DTPA, {sup 99m}Tc-DMSA, {sup 99m}Tc-pyrophosphate, {sup 99m}Tc-phytic acid, {sup 99m}Tc-MDS, {sup 99m}Tc-HMDP) were done by reversed-phaseion pairing chromatography using a optimized mobile phase consisted on a mixture of 50 mM phosphate buffer (pH 7.0) and 2 mM TBA (tetra nbutyl) ammonium hydroxide in 30 % methanol. The mobile phases for analysis of medium molecular weight {sup 99m}Tc-HSA were consisted of a mixture of 50 mM phosphate buffer (ph 7.0) in 30 % methanol, and a mixtures of 1 % SDS (sodium dodecyl sulfonate) in Tris buffer (pH. 7.0), respectively. It was apparent from the radio-chromatograms obtained from these chromatographic conditions, that impurity of {sup 99m}TcO{sub 4} was observed in {sup 99m}Tc-pyrophosphate, {sup 99m}Tc-phytic acid, {sup 99m}Tc-MDP, {sup 99m}Tc-HMDP, and impurities of {sup 99m}Tc-labeled species and {sup 99m}TcO{sub 4}, were observed in {sup 99m}Tc-HIDA, {sup 99m}Tc-HIDA, {sup 99m}Tc-HSA. The radiochemical impurities of the {sup 99m}Tc-radiopharmaceuticals were ranged between 90 and 100 %. From these results, radio-HPLC has been shown to be suitable method for analysis of {sup 99m}Tc-radiopharmaceuticals, with rapidity and excellent precision. (author).

  7. Scatter and attenuation correction in Technetium-99m brain SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Hashimoto, Jun [Keio Univ., Tokyo (Japan). School of Medicine

    1997-11-01

    The imaging in the nuclear medicine is inferior to other modalities in the spatial resolution, but it can quantitatively evaluate the biological function. The attempt to measure the absolute or the relative values of local functions quantitatively based on the SPECT of the brain or the heart, has become popular recently. It is not only necessary to introduce an appropriate analytical model for each radioisotope tracer for the correct quantification, but also to be equipped with the photographing system to measure correctly the accumulation of the tracer. The main cause to decrease the precision of the photographing system is the scatter and the absorption (attenuation) of photon. In the present study we attempted to enhance the quantification of the brain SPECT by the usable and practical correction method for the scatter and the attenuation in the daily clinical business. We used the triple-energy-window (TEW) method as the scatter correction and the attenuation calculation map obtained from the transmission scanning (TCT) by the external source as the attenuation correction. We examined the brain SPECT in combination with these two correction methods. We could not measure correctly the amount of the tracer accumulation by only one correction method, and obtain the excellent quantitative SPECT images in combination with both correction methods. We could get excellently stable data by collecting the emission and the transmission data simultaneously as well as separately, and the excellent images by the arithmetical treatment of data between the scatter beam and the detector correction when we used {sup 99m}Tc as the source which is economically cheap, and adopted the combination of the tracers labeled by the same kind of radioisotopes. Altogether we expect that our method is very practical and will contribute to enhance the accuracy in quantitative analysis of clinical brain SPECT imaging. (K.H.)

  8. Technetium-99m-sestamibi scintigraphy in breast nodes; Estudo cintilografico de nodulos mamarios com tecnecio-99m-sestamibi

    Energy Technology Data Exchange (ETDEWEB)

    Pellini, Marcos Pinto; Fonseca, Lea Mirian Barbosa da [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina; Balen, Jacir Luiz; Fabricio, Maria Ines Menescal [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina. Inst. de Ginecologia; Amarante Junior, Jose Luiz de Medeiros

    1997-01-01

    The purpose was to was to determine if technetium-99m-sestamibi accumulates preferentially within the malignant palpable nodes of breast. Twenty-five patients, mean age 36.16 ({+-} 9.34) year, and without any other additional information, underwent breast scintigraphy and excisional biopsy. We had nineteen true-negative cases, four true-positive, two false-positive and none false-negative. Sensitivity, 100% specificity, 90.5%, accuracy, 92%; PPV, 66.7%, NPV, 100%. The four true positive were invasive intraductal carcinomas and one of them metastases in auxiliary lymph-nodes, confirmed in biopsy and well defined in scintigraphy. The two false-positives were a fibroadenoma with high cellularity and a fibrodisplasy surrounded by chronic inflammatory process. Two statistical tests were applied: chi-square and Fisher. Both rejected the antithesis at a confidence interval of 99% (p , 0.01) We concluded that technetium-99-sestamibi accumulates preferentially within the malignant nodes of breast. (author) 17 refs., 3 figs., 2 tabs.

  9. Lessons from the Tc-99m shortage: implications of substituting Tl-201 for Tc-99m single-photon emission computed tomography.

    Science.gov (United States)

    Small, Gary R; Ruddy, Terrence D; Simion, Oana; Alam, Mohammed; Fuller, Lyanne; Chen, Li; Beanlands, Rob S; Chow, Benjamin J W

    2013-09-01

    In 2009, the Chalk River nuclear reactor closed for repairs that led to a critical shortage of technetium-99m (Tc-99m). Several centers used thallium-201 (Tl-201) as an alternative radiotracer for myocardial perfusion imaging. Because Tl-201 is considered by many as a suboptimal radiotracer, we sought to understand the impact of using Tl-201 (during the Tc-99m shortage) on downstream resource utilization. We performed a retrospective study at the Ottawa Heart Institute of 7402 patients (60% men; mean age, 62.6 ± 11.8 years), patients were referred for myocardial perfusion imaging between May 2008 and January 2011 (PRE_Tc-99m [2938 patients]), during (DURING_Tl-201 [2959 patients]), and after (POST_Tc-99m [1505 patients]) the Tc-99m shortage. Patients were followed for 6 months after their index myocardial perfusion imaging to determine subsequent rates of cardiac catheterization or noninvasive imaging. More downstream testing was seen in the Tl-201 cohort (639 [21.4%] patients) than the Tc-99m cohort (537 [12.1%] patients; PTl-201 cohort compared with the Tc-99m cohort ($90.97; 95% confidence interval, 83.42-98.90; PTl-201 (23.57 mSv; 95% confidence interval, 23.16-23.96) than in Tc-99m (12.92 mSv; 95% confidence interval, 12.55-13.40; PTl-201 during the Tc-99m shortage was associated with an increase in downstream testing, cost, and patient radiation exposure, but these findings may not be generalizable to other centers. Although Tl-201 provided a short-term solution to the unexpected Tc-99m shortage, long-term cost-effective solutions should be areas of future study.

  10. Synthesis of specific SPECT-radiopharmaceutical for tumor imaging based on methionine: 99mTc-DTPA-bis(methionine).

    Science.gov (United States)

    Hazari, Puja Panwar; Shukla, Gauri; Goel, Vijay; Chuttani, Krishna; Kumar, Nitin; Sharma, Rajnish; Mishra, Anil Kumar

    2010-02-17

    Methionine-diethylenetriaminepentaaceticacid-methionine [DTPA-bis(Met)] was synthesized by covalently conjugating two molecules of methionine (Met) to DTPA and was labeled with (99m)Tc in high radiochemical purity and specific activity (166-296 MBq/micromol). Kinetic analysis showed K(m) of 12.95 +/- 3.8 nM and a maximal transport rate velocity (V(max)) of 80.35 +/- 0.42 pmol microg protein(-1) min(-1) of (99m)Tc-DTPA-bis(Met) in U-87MG cells. DTPA-bis(Met) had dissociation constants (K(d)) of 0.067 and 0.077 nM in U-87MG and BMG, respectively. (35)S-methionine efflux was trans-stimulated by (99m)Tc-labeled DTPA conjugate demonstrating concentrative transport. The blood kinetic studies showed fast clearance with t(1/2) (F) = 36 +/- 0.5 min and t(1/2) (S) = 5 h 55 min +/- 0.85 min. U-87MG and BMG tumors saturated at approximately 2000 +/- 280 nmol/kg of (99m)Tc-DTPA-bis(Met). Initial rate of transport of (99m)Tc-DTPA-bis(Met) in U-87MG tumor was found to be 4.68 x 10(-4) micromol/kg/min. The tumor (BMG cell line, malignant glioma) grafted in athymic mice were readily identifiable in the gamma images. Semiquantitative analysis from region of interest (ROI) placed over areas counting average counts per pixel with maximum radiotracer uptake on the tumor was found to be 11.05 +/- 3.99 and compared ROI with muscle (0.55 +/- 0.13). The tumor-to-contralateral muscle tissue ratio of (99m)Tc-DTPA-bis(Met) was found to be 23 +/- 3.3. Biodistribution revealed significant tumor uptake and good contrast in the U-87MG, BMG, and EAT tumor-bearing mice. In clinical trials, the sensitivity, specificity, and positive predictive values were found to be 87.8%, 92.8%, and 96.6%, respectively. (99m)Tc-DTPA-bis(Met) showed excellent tumor targeting and has promising utility as a SPECT-radiopharmaceutical for imaging methionine-dependent human tumors and to quantify the ratio of MET(+)/HCY(-).

  11. Development and validation of methodology for technetium-99m radiopharmaceuticals using high performance liquid chromatography (HPLC); Desenvolvimento e validacao de metodologia para radiofarmacos de tecnecio-99m empregando cromatografia liquida de alta eficiencia (CLAE)

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Erika Vieira de

    2009-07-01

    Radiopharmaceuticals are compounds, with no pharmacological action, which have a radioisotope in their composition and are used in Nuclear Medicine for diagnosis and therapy of several diseases. In this work, the development and validation of an analytical method for {sup 99}mTc-HSA, {sup 99}mTc-EC, {sup 99}mTc-ECD and {sup 99}mTc-Sestamibi radiopharmaceuticals and for some raw materials were carried out by high performance liquid chromatography (HPLC). The analyses were performed in a Shimadzu HPLC equipment, LC-20AT Prominence model. Some impurities were identified by the addition of a reference standard substance. Validation of the method was carried out according to the criteria defined in RE n. 899/2003 of the National Sanitary Agency (ANVISA). The results for robustness of the method showed that it is necessary to control flow rate conditions, sample volume, pH of the mobile phase and temperature of the oven. The analytical curves were linear in the concentration ranges, with linear correlation coefficients (r{sup 2}) above 0.9995. The results for precision, accuracy and recovery showed values in the range of 0.07-4.78%, 95.38-106.50% and 94.40-100.95%, respectively. The detection limits and quantification limits varied from 0.27 to 5.77 {mu}g mL{sup -1} and 0.90 to 19.23 {mu}g mL{sup -1}, respectively. The values for HAS, EC, ECD and MIBI in the lyophilized reagents were 8.95; 0.485; 0.986 and 0.974 mg L-1, respectively. The mean radiochemical purity for {sup 99}mTc-HSA, {sup 99}mTc-EC, {sup 99}mTc-ECD and {sup 99}mTc-Sestamibi was (97.28 {+-} 0.09)%, (98.96 {+-} 0.03)%, (98.96 {+-} 0.03)% and (98.07 {+-} 0.01)%, respectively. All the parameters recommended by ANVISA were evaluated and the results are below the established limits. (author)

  12. Potential value of color-coded dynamic breast-specific gamma-imaging; comparing {sup 99m}Tc-(V)-DMSA, {sup 99m}Tc-MIBI, and {sup 99m}Tc-HDP in a mouse mammary tumor model

    Energy Technology Data Exchange (ETDEWEB)

    Leeuwen, Fijs W.B. van, E-mail: fw.v.leeuwen@nki.n [Departments of Radiology and Nuclear Medicine, Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, 1066 CX Amsterdam (Netherlands); Buckle, Tessa; Batteau, Lukas; Pool, Bert; Sinaasappel, Michiel [Departments of Radiology and Nuclear Medicine, Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, 1066 CX Amsterdam (Netherlands); Jonkers, Jos [Department of Molecular Biology, Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, 1066 CX Amsterdam (Netherlands); Gilhuijs, Kenneth G.A. [Departments of Radiology and Nuclear Medicine, Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, 1066 CX Amsterdam (Netherlands)

    2010-12-15

    Using a mouse mammary tumor model based on orthotopic transplantation of luciferase-expressing mouse ILC cells (KEP1-Luc cells), we evaluated the diagnostic value of three clinically applied tracers: {sup 99m}Tc-(V)-DMSA, {sup 99m}Tc-MIBI, and {sup 99m}Tc-HDP. Uptake of the tracers is compared using static and dynamic imaging procedures. We found that dynamic imaging in combination with pixel-by-pixel color coding has an added value over (high resolution) static imaging procedures. Such dynamic imaging procedures could enhance the potential of breast-specific gamma-imaging.

  13. (99m)Tc-Galacto-RGD2: a novel 99mTc-labeled cyclic RGD peptide dimer useful for tumor imaging.

    Science.gov (United States)

    Ji, Shundong; Czerwinski, Andrzej; Zhou, Yang; Shao, Guoqiang; Valenzuela, Francisco; Sowiński, Paweł; Chauhan, Satendra; Pennington, Michael; Liu, Shuang

    2013-09-01

    This study sought to evaluate [(99m)Tc(HYNIC-Galacto-RGD2)(tricine)(TPPTS)] ((99m)Tc-Galacto-RGD2: HYNIC = 6-hydrazinonicotinyl; Galacto-RGD2 = Glu[cyclo[Arg-Gly-Asp-D-Phe-Lys(SAA-PEG2-(1,2,3-triazole)-1-yl-4-methylamide)

  14. Comparison of differential renal function using technetium-99m mercaptoacetyltriglycine (MAG3) and technetium-99m dimercaptosuccinic acid (DMSA) renography in a paediatric population

    Energy Technology Data Exchange (ETDEWEB)

    Ritchie, Gillian; Wilkinson, Alistair G. [Royal Hospital for Sick Children, Edinburgh (United Kingdom); Prescott, Robin J. [University of Edinburgh Medical School, Medical Statistics Unit, Public Health Sciences, Edinburgh (United Kingdom)

    2008-08-15

    In children who have undergone both {sup 99m}Tc-DMSA and {sup 99m}Tc-MAG3 studies for the assessment of differential renal function (DRF) and drainage, respectively, we have noticed good agreement between the calculated DRF values, and hypothesized that there is no significant difference in DRF values calculated from these tests. Therefore, both tests may not always be necessary. To determine whether there is a statistically significant difference between DRF values calculated using {sup 99m}Tc-DMSA and those calculated using {sup 99m}Tc-MAG3. We retrospectively identified children imaged with {sup 99m}Tc-DMSA and {sup 99m}Tc-MAG3. We recorded DRF values, age, indication, and renal pelvis diameter. For the {sup 99m}Tc-DMSA studies we recorded the imaging time after injection. For the {sup 99m}Tc-MAG3 studies we recorded the delay between injection and data acquisition, diuretic use and evidence of delayed drainage or reflux. We identified 100 episodes in 92 children where both {sup 99m}Tc-DMSA and {sup 99m}Tc-MAG3 scans had been performed within a few days. The commonest indication was urinary tract infection or pelviureteric junction obstruction. The mean age of the children was 6.96 years. A significant but clinically acceptable trend was seen between abnormal DRF and difference between tests. A significant link was found with the difference between tests and the time of imaging after DMSA injection, and also with scarring. No significant effect was caused by renal pelvis dilatation, delayed drainage, frusemide administration, or delayed {sup 99m}Tc-MAG3 imaging. If a {sup 99m}Tc-MAG3 study has been performed then a {sup 99m}Tc-DMSA study is unnecessary provided DRF is normal on the {sup 99m}Tc-MAG3 study and there is no scarring. A change in practice would lead to considerable savings in time, cost and radiation burden. (orig.)

  15. /sup 99m/Tc-aminohexylidendiphosphonate and /sup 99/mTc-Pyrophosphate in the scintigraphic diagnosis of experimental cardiomyopathy in dogs

    Energy Technology Data Exchange (ETDEWEB)

    Duska, F.; Kafka, P.; Mazurova, Y.; Hadas, L.; Vizda, J.; Palicka, V.; Grossman, V.

    1987-10-01

    Experimental cardiomyopathy was provoked in 24 dogs with high intravenous doses of adrenaline and theophylline. These lesions were studied by means of the new agent /sup 99m/Tc-AHDP and /sup 99m/Tc-PYP in comparison. Cardiomyopathy could be imaged as early as 4 h after the onset of involvement but not later than 7 days. A maximum accumulation occurred in lesions 24 h old, /sup 99m/Tc uptake in the myocardium was graded scintigraphically, /sup 99m/Tc-AHDP was accumulated in the altered myocardium to a greater extent than /sup 99m/Tc-PYP. Scintigraphic findings were in good agreement with plasma levels of creatine-kinase. A comparison with histology demonstrated that the maximum accumulation of radiopharmaceuticals occurred at the time when the development of myocardium involvement reached the stage of myocytolysis.

  16. Preclinical Validation of 99mTc–Annexin A5–128 in Experimental Autoimmune Myocarditis and Infective Endocarditis: Comparison with 99mTc–HYNIC–Annexin A5

    Directory of Open Access Journals (Sweden)

    Khadija Benali

    2015-01-01

    Full Text Available Hydrazinonicotinamide–annexin A5 (HYNIC-Anx, a 99m technetium (99mTc-labeled agent targeting phosphatidylserine, proved to be sensitive for the detection of apoptosis and thrombosis but is no longer available for clinical use. A mutant of human annexin designed for direct 99mTc labeling (referred to as Anx A5–128 showed improved binding affinity to phosphatidylserine and is expected to be used in humans. We compared both radiotracers with regard to pharmacokinetics and diagnostic ability in animal models. Biodistribution studies were performed in normal rats. Radiolabeled Anx A5–128 and HYNIC-Anx were compared in cardiovascular settings involving phosphatidylserine expression: experimental autoimmune myocarditis and infective endocarditis. Initial blood clearance was faster for Anx A5–128 than for HYNIC-Anx, and tissue biodistribution was similar overall for both tracers. The diagnostic sensitivity of Anx A5–128 was excellent and comparable to that of HYNIC-Anx. Anx A5–128 showed biodistribution and diagnostic ability similar to those of the HYNIC-Anx derivative, supporting its translation to clinical use.

  17. A Novel Ternary Ligand System Useful for Preparation of Cationic 99mTc-Diazenido Complexes and 99mTc-Labeling of Small Biomolecules

    Science.gov (United States)

    Kim, Young-Seung; He, Zhengjie; Hsieh, Wen-Yuan; Liu, Shuang

    2008-01-01

    This report describes a novel ternary ligand system composed of a phenylhydrazine, a crown ether-containing dithiocarbamate (DTC) and a PNP-type bisphosphine (PNP). The combination of three different ligands with 99mTc results in cationic 99mTc-diazenido complexes,[99mTc(NNAr)(DTC)(PNP)]+, with potential radiopharmaceuticals for heart imaging. Synthesis of cationic 99mTc-diazenido complexes can be accomplished in two steps with high yield. For example, the reaction of phenylhydrazine with 99mTcO4− at 100 °C in the presence of excess stannous chloride and 1,2-diaminopropane-N,N,N’,N’-tetraacetic acid (PDTA) results in the [99mTc(NNPh)(PDTA)n] intermediate, which then reacts with sodium N-(dithiocarbamato)-2-aminimethyl-15-Crown-5 (L4) and N,N-bis[2-(bis(3-ethoxypropyl)phosphino)ethyl]ethoxy-ethylamine (PNP6) at 100 °C for 15 min to give the complex, [99mTc(NNPh)(L4)(PNP6)]+ in high yield (> 90%). Cationic complexes [99mTc(NNPh)(DTC)(PNP)]+ are stable for ≥6 h. Their composition was determined to be 1:1:1:1 for Tc:NNPh:DTC:PNP using the mixed-ligand experiments on the tracer (99mTc) level, and was further confirmed by the ESI-MS spectral data of a model compound [Re(NNPh)(L4)(L6)]+. It was found that both DTCs and bisphosphines have a significant impact on the lipophilicity of their cationic 99mTc-diazenido complexes. Results from a 99mTc-labeling efficiency experiment showed that 4-hydrazinobenzoic acid (HYBA) might be useful as a bifunctional coupling agent for 99mTc-labeling of small biomolecules. However, the 99mTc-labeling efficiency of HYBA is much lower than that of 6-hydrazinonicotinic acid (HYNIC) with tricine and trisodium triphenylphosphine-3,3’,3”-trisulfonate (TPPTS) as coligands. PMID:16536480

  18. Brain PET and technetium-99m-ECD SPECT imaging in Lhermitte-Duclos disease

    Energy Technology Data Exchange (ETDEWEB)

    Ogasawara, K.; Yasuda, S.; Beppu, T.; Kobayashi, M.; Doi, M.; Kuroda, K.; Ogawa, A. [Dept. of Neurosurgery, Iwate Medical Univ., Morioka (Japan)

    2001-11-01

    Two patients with Lhermitte-Duclos disease were evaluated by brain positron emission tomography (PET) and technetium-99m-ethyl cysteinate dimer ({sup 99m}Tc-ECD) single-photon emission computed tomography (SPECT). In the lesions in both patients, hyperperfusion was detected on cerebral blood flow images obtained by PET, and hyperactivity by standard {sup 99m}Tc-ECD SPECT. Dynamic {sup 99m}Tc-ECD SPECT images demonstrated a plateau of activity in each lesion. These findings suggest that lesions in Lhermitte-Duclos disease have a retention mechanism for {sup 99m}Tc-ECD equivalent to that of normal neural tissue. (orig.)

  19. Comparison of efficacy of Tc-99m HIG and Tc-99m nanocolloid on sentinel lymph node mapping in patients with breast cancer.

    Science.gov (United States)

    Yalçin, Hulya; Gençoglu, Esra Arzu; Yagmurdur, Mahmut; Aras, Murat; Ozen, Aynur; Aktas, Ayse

    2010-10-01

    This study compared the effectiveness of Tc-99m human polyclonal immunoglobulin (HIG) and Tc-99m nanocolloid at detecting sentinel lymph nodes (SLNs) with lymphoscintigraphy and an intraoperative gamma-probe (IGP) in patients with early breast cancer. The study group consisted of 50 women; 25 patients each were given Tc-99m HIG or Tc-99m nanocolloid for lymphoscintigraphy. Then, intraoperative SLN localization with IGP was performed. The results of IGP, lymphoscintigraphy, blue dye injected just before surgery, and pathology were compared. In the Tc-99m HIG group, one patient had tumours in both breasts. In two patients, we could not detect SLNs with lymphoscintigraphy, although they were detected with IGP and blue dye. We found SLNs for all tumours with IGP. With the intraoperative blue dye, SLNs were identified for 25 tumours; for one tumour, no SLN was detected with blue dye. In the histopathological examination, 13 tumours showed metastasis in the SLN and in 11 of these 13, there were also metastases in the axilla. One patient had a skip metastasis. In the Tc-99m nanocolloid group, SLNs were identified in 24 patients with lymphoscintigraphy. IGP found SLNs in 24 patients. The blue dye detected SLNs in all patients. On histopathological examination, 10 patients had metastasis in the SLN and there were also metastases in the axilla in all of these patients. Tc-99m HIG can be used in SLN detection with preoperative lymphoscintigraphy and IGP in early-stage breast cancer patients.

  20. Assessment of the direct cyclotron production of (99m)Tc: An approach to crisis management of (99m)Tc shortage.

    Science.gov (United States)

    Rovais, Mohammad Reza Aboudzadeh; Aardaneh, Khosro; Aslani, Gholamreza; Rahiminejad, Ali; Yousefi, Kamran; Boulouri, Fatemeh

    2016-06-01

    Nowadays, the cyclotron production of technetium-99m ((99m)Tc) has been increased, due to the worldwide (99m)Tc generator shortage. In the present work, an improved strategy for the production of (99m)Tc, using the proton irradiation of the enriched (100)Mo was developed. The performance of this method in terms of the production yield, chemical purity, radiochemical purity, as well as radionuclide purity was evaluated. The average production yield was measured to be 356MBqμA(-1)h(-1). A good agreement was found between the calculated production yield and the experimental one. The radiochemical separation and total recovery yields of (99m)Tc were 92% and 69%, respectively. The radiochemical and the radionuclide purities of the (99m)Tc were 99% and >99.99% at the end of purification, respectively. The results of quality control tests (QC) support the concept that cyclotron-produced (99m)Tc is suitable for preparation of USP-compliant.

  1. Investigation of four {sup 99m}Tc-labeled bacteriophages for infection-specific imaging

    Energy Technology Data Exchange (ETDEWEB)

    Rusckowski, Mary [Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655-0243 (United States)], E-mail: mary.rusckowski@umassmed.edu; Gupta, Suresh; Liu Guozheng; Dou Shuping; Hnatowich, Donald J. [Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655-0243 (United States)

    2008-05-15

    Introduction: This study investigated radiolabeled bacteriophages for specific detection of infection through gamma imaging. Previously, a {sup 99m}Tc-labeled M13 phage demonstrated specific binding for its host Escherichia coli in vitro and in mice through imaging. Methods: This study was extended to phages P22, E79, VD-13 and phage 60. Each was radiolabeled with {sup 99m}Tc using the chelator MAG{sub 3}, and were evaluated for binding to host and non-host bacteria in vitro and in a mouse infection model. Results: In vitro, each {sup 99m}Tc-phage bound to its host at least 4-fold higher than to non-host bacteria. For example, {sup 99m}Tc-E79 showed 10- to 20-fold greater binding to host Pseudomonas aeruginosa compared to non-host Escherichia coli and Salmonella enterica, and {sup 99m}Tc-phage 60 showed 20-fold greater binding to host Klebsiella pneumoniae over non-hosts. Mice received host or non-host bacteria in one thigh, and 3 h later, the {sup 99m}Tc-phages were administered intravenously. After a further 3 h, the tissues were counted. Liver accumulation was highest for {sup 99m}Tc-E79, averaging 39% compared to an average of 13% for the other {sup 99m}Tc-phages. Animals infected with host bacteria showed infected thigh/normal thigh ratios of 14.2 for {sup 99m}Tc-E79, 2.9 for {sup 99m}Tc-P22, 3.5 for {sup 99m}Tc-VD-13 and 2.1 for {sup 99m}Tc-phage 60. Conclusions: Although specific host binding was observed in vitro for each of these four {sup 99m}Tc-phages, only {sup 99m}Tc-E79 showed specificity for its host in an in vivo model.

  2. PEGylation of {sup 99m}Tc-labeled bombesin analogues improves their pharmacokinetic properties

    Energy Technology Data Exchange (ETDEWEB)

    Daepp, Simone; Garayoa, Elisa Garcia [Paul Scherrer Institute, Center for Radiopharmaceutical Sciences ETH-PSI-USZ, CH-5232 Villigen-PSI (Switzerland); Maes, Veronique; Brans, Luc; Tourwe, Dirk A. [Department of Organic Chemistry, Vrije Universiteit Brussel, 1050 Brussels (Belgium); Mueller, Cristina [Paul Scherrer Institute, Center for Radiopharmaceutical Sciences ETH-PSI-USZ, CH-5232 Villigen-PSI (Switzerland); Schibli, Roger, E-mail: roger.schibli@psi.ch [Paul Scherrer Institute, Center for Radiopharmaceutical Sciences ETH-PSI-USZ, CH-5232 Villigen-PSI (Switzerland); Department of Chemistry and Applied Biosciences, ETH Zurich, 8093 Zurich (Switzerland)

    2011-10-15

    Introduction: Radiolabeled bombesin (BN) conjugates are promising radiotracers for imaging and therapy of breast and prostate tumors in which BN{sub 2}/gastrin-releasing peptide (GRP) receptors are overexpressed. However, the low in vivo stability of BN conjugates may limit their clinical application. In an attempt to improve their pharmacokinetics and counteract their rapid enzymatic degradation, we prepared a series of polyethylene glycol (PEG)-ylated BN(7-14) analogues for radiolabeling with {sup 99m}Tc(CO){sub 3} and evaluated them in vitro and in vivo. Methods: Derivatization of a stabilized (N{sup {alpha}H}is)Ac-BN(7-14)[Cha{sup 13},Nle{sup 14}] analogue with linear PEG molecules of various sizes [5 kDa (PEG{sub 5}), 10 kDa (PEG{sub 10}) and 20 kDa (PEG{sub 20})] was performed by PEGylation of the {epsilon}-amino group of a {beta}{sup 3}hLys-{beta}Ala-{beta}Ala spacer between the stabilized BN sequence and the (N{sup {alpha}H}is)Ac chelator. The analogues were then radiolabeled by employing the {sup 99m}Tc-tricarbonyl technique. Binding affinity and internalization/externalization studies were performed in vitro in human prostate carcinoma PC-3 cells. Stability was investigated in vitro in human plasma and in vivo in Balb/c mice. Finally, single photon emission computed tomography (SPECT)/X-ray computed tomography studies were performed in nude mice bearing PC-3 tumor xenografts. Results: PEGylation did not affect the binding affinity of BN analogues, as the binding affinity for BN{sub 2}/GRP receptors remained high (K{sub d}<0.9 nM). However, in vitro binding kinetics of the PEGylated analogues were slower. Steady-state condition was reached after 4 h, and the total cell binding was 10 times lower than that for the non-PEGylated counterpart. Besides, PEGylation improved the stability of BN conjugates in vitro and in vivo. The BN derivative conjugated with a PEG{sub 5} molecule showed the best pharmacokinetics in vivo, i.e., faster blood clearance and

  3. {sup 99m}Tc-MDP and {sup 99m}Tc-MIBI scintimammography in patients with clinically suspicious breast cancer

    Energy Technology Data Exchange (ETDEWEB)

    Pai, Moon Sun; Park, Chan H.; Kim, Su Zy; Yoon, Suk Nam; Park, Hee Boong [College of Medicine, Ajou Univ., Suwon (Korea, Republic of)

    1997-07-01

    {sup 99m}Tc-MDP scintimammography(SM) was performed at the time of the preoperative bone scan in patients with suspicious breast cancer. These patients also had {sup 99m}Tc-MIBI scintimammography before or after the bone scan. The purpose of this report is to compare {sup 99m}Tc-MDP SM and {sup 99m}Tc-MIBI SM with the surgical results. Scintimammography with {sup 99m}Tc-MDP was performed in 20 patients with palpable mass and radiologic suspicion or proven diagnosis of breast cancer preoperatively. Patients ranged in age from 34 to 72. No male patient was included. Both prone lateral and supine anterior views were obtained for 10 min each 10-15min after IV administration of 740 MBq of {sup 99m}Tc-MDP. The {sup 99m}Tc-MIBI scintimammography was also performed before surgery. {sup 99m}Tc-MIBI SM began in 5 min. postinjection of 740MBq of {sup 99m}Tc-MIBI with prone lateral views for 10 min. Scintimammography was evaluated as positive or negative for presence or absence of focal localization of the tracers and the results were compared to histological findings. Breast cancer was histologically diagnosed in all patients ( 16 infiltrating ductal carcinoma, 2 comedocarcinoma, 1 phyllodes tumor, 1 paget disease). The size of tumor mass ranged 1.5-3 cm. Lymph node metastasis was found in 4 patients by the histologic examination. The uptake of {sup 99m}Tc-MDP in breast cancer was noted in all patients except two. One of the two patients was in post excisional biopsy state and the other was also negative in the MIBI scan. There was MDP axillary node uptake in 5 patients. Two of 5 patients with axillary lymph node uptake were false positive but they were all positive with {sup 99m}Tc-MIBI scintimammography. Two masses found in MIBI scan of a patients cannot be separated o the MDP scan. It showed more diffuse uptake than MIBI scan. The concordance between MDP-SM with MIBI-SM(similar pattern of uptake) was 45% and the remaining cases showed not focal but more diffuse uptake

  4. (99m)Tc sulfur colloid and (99m)Tc mebrofenin hepatobiliary functional liver imaging in normal and diabetic rats.

    Science.gov (United States)

    Al-Saeedi, Fatma; Loutfi, Issa

    2011-01-01

    To use (99m)Tc sulfur colloid ((99m)Tc-SC) and (99m)Tc mebrofenin ((99m)Tc-BrIDA) to study liver function in normal and diabetic rats. Radionuclide imaging was performed on 2 groups of rats, using (99m)Tc-SC for one group and (99m)Tc-BrIDA for the other (20 rats per group) before and after induction of diabetes mellitus (DM) using streptozotocin administration (55 mg/kg i.p.). Dynamic acquisition was obtained for 1 h after the injection of 37 MBq of radiotracer. For the (99m)Tc-SC group, organ/tissue uptake was determined by drawing regions of interest (ROI) over the heart, liver, spleen and also the whole body (WB). The ratio of the ROI of each organ to the WB ROI was calculated. For (99m)Tc-BrIDA, ratios of cumulative count rates in liver, liver parenchyma, biliary tree and abdomen ROI to a WB ROI were also calculated. Statistical analysis was performed to compare the ratios of organ/tissue uptake to WB uptake before and after DM induction using the paired t test. (99m)Tc-SC uptake ratios (means ±SD) showed a lower liver-to-WB uptake ratio (0.75 ± 0.05) in the rats after DM induction compared to baseline (0.81 ± 0.06), while the cardiac blood pool showed higher uptake ratios in the rats after DM induction (p = 0.026). For (99m)Tc-BrIDA, there was no significant difference in radiotracer uptake ratios obtained from the rats before and after DM induction (p = 0.41). Using functional liver imaging, there was a statistically significant decrease in the liver phagocytic/reticuloendothelial system function after DM induction, as evidenced by decreased (99m)Tc-SC liver uptake and increased blood pool compared to prediabetes, while the hepatobiliary function remained unchanged after DM induction using (99m)Tc-BrIDA imaging. Copyright © 2011 S. Karger AG, Basel.

  5. RP463: a stabilized technetium-99m complex of a hydrazino nicotinamide derivatized chemotactic peptide for infection imaging.

    Science.gov (United States)

    Edwards, D S; Liu, S; Ziegler, M C; Harris, A R; Crocker, A C; Heminway, S J; Barrett, J A; Bridger, G J; Abrams, M J; Higgins, J D

    1999-01-01

    A HYNIC-conjugated chemotactic peptide (fMLFK-HYNIC) was labeled with (99m)Tc using tricine and TPPTS as coligands. The combination of fMLFK-HYNIC, tricine, and TPPTS with (99m)Tc produced a ternary ligand complex [(99m)Tc(fMLFK-HYNIC)(tricine)(TPPTS)] (RP463). RP463 was synthesized either in two steps, in which the binary ligand complex [(99m)Tc(fMLFK-HYNIC)(tricine)(2)] (RP469) was formed first and then reacted with TPPTS, or in one step by direct reduction of [(99m)Tc]pertechnetate with stannous chloride in the presence of fMLFK-HYNIC, tricine, and TPPTS. The radiolabeling yield for RP463 was usually >/=90% using 10 microg of fMLFK-HYNIC and 100 mCi of [(99m)Tc]pertechnetate. Unlike RP469, which decomposed rapidly in the absence of excess tricine coligand, RP463 was stable in solution for at least 6 h. [(99)Tc]RP463 was prepared and characterized by HPLC and electrospray mass spectrometry. In an in vitro assay, [(99)Tc]RP463 showed an IC(50) of 2 nM against binding of [(3)H]fMLF to receptors on PMNs. [(99)Tc]RP463 also induces effectively the superoxide release of polymorphonuclear leukocytes (PMNs) with an EC(50) value of 0.2 +/- 0.2 nM. The localization of RP463 in the infection foci was assessed in a rabbit infection model. RP463 was cleared from the blood faster than RP469 and was excreted mainly through the renal system. As a result of rapid blood clearance and increased uptake, the target-to-background ratios continuously increased from 1.5 +/- 0.2 at 15 min postinjection to 7.5 +/- 0.4 at 4 h postinjection. Visualization of the infected area could be as early as 2 h. A transient decrease in white blood cell count of 35% was observed during the first 30 min after injection of the HPLC-purified RP463 in the infected rabbit. This suggests that future research in this area should focus on developing highly potent antagonists for chemotactic peptide receptor or other receptors on PMNs and monocytes.

  6. [sup 99m]Tc-ECD SPECT study in dementia and aphasia

    Energy Technology Data Exchange (ETDEWEB)

    Kuwabara, Yasuo; Ichiya, Yuichi; Otsuka, Makoto; Sasaki, Masayuki; Akashi, Yuko; Fukumura, Toshimitsu; Yoshida, Tsuyoshi; Masuda, Kouji; Ichimiya, Atsushi (Kyushu Univ., Fukuoka (Japan). Faculty of Medicine)

    1993-09-01

    We studied clinical significance of [sup 99m]Tc-L,L,-ethyl cysteine dimer ([sup 99m]Tc-ECD) SPECT study in dementia and aphasia, and compared it with [sup 99m]Tc-HMPAO SPECT study. The subjects consisted of 13 patients, including 10 patients with dementia and 3 patients with aphasia. Hypoperfusion areas were detected in 5 out of 10 patients with dementia and 2 out of 3 patients with aphasia in [sup 99m]Tc-ECD SPECT, and in 4 out of 10 patients with dementia and all of 3 patients with aphasia in [sup 99m]Tc-HMPAO SPECT. The count rate ratios in [sup 99m]Tc-ECD and [sup 99m]Tc-HMPAO SPECT were correlated well with each other, and the contrast of the [sup 99m]Tc-ECD SPECT image was equivalent or slightly higher as compared with [sup 99m]Tc-HMPAO. Therefore, [sup 99m]Tc-ECD SPECT study was considered to be useful for the evaluation of cerebral perfusion in dementia and aphasia. (author).

  7. sup(99m)Tc-Ethylenediamine-N, N-diacetic acid. A radiopharmaceutical with tumor affinity

    Energy Technology Data Exchange (ETDEWEB)

    Imoto, Takeshi (Kyushu Univ., Fukuoka (Japan). Faculty of Medicine)

    1984-05-01

    sup(99m)Tc-Ethylenediamine-N, N-Diacetic Acid (sup(99m)Tc-EDDA), a compound having affinity for tumors was evaluated, and the results were as follows. 1) sup(99m)Tc-EDDA accumulated in experimentally-induced tumors. The tumor/tissue ratio (%g/dose) of sup(99m)Tc-EDDA was greater than that of /sup 67/Ga-citrate, especially with Ehrlich ascites tumors. 2) sup(99m)Tc-EDDA accumulated mainly in the nuclear components of tumor cells. 3) No acute toxic effects of EDDA were observed in mice, rabbits or in volunteers. 4) The sup(99m)Tc-EDDA image was positive in 72 % of clinical examinations of lung cancer. The sup(99m)Tc-EDDA did not accumulate in the liver, and this suggests that tumor near the liver may be more easily detected with sup(99m)Tc-EDDA than with /sup 67/Ga-citrate. 5) In some cases sup(99m)Tc-EDDA accumulated in mammary glands (nipples) and in pleural effusions (pleuritis), though the mechanism of which was obscure. 6) sup(99m)Tc-EDDA is a good agent for detecting malignant tumors.

  8. Absorbed dose at subcellular level by Monte Carlo simulation for a {sup 99m}Tc-peptide with nuclear internalization

    Energy Technology Data Exchange (ETDEWEB)

    Rojas C, E. L.; Ferro F, G. [ININ, Carretera Mexico-Toluca s/n, Ocoyoacac 52750, Estado de Mexico (Mexico); Santos C, C. L., E-mail: leticia.rojas@inin.gob.m [Universidad Autonoma del Estado de Mexico, Paseo Tollocan esquina Paseo Colon s/n, Toluca 50120, Estado de Mexico (Mexico)

    2010-10-15

    The utility of radiolabeled peptides for the early and specific diagnosis of cancer is being investigated around the world. Recent investigations have demonstrated the specificity of {sup 99m}Tc-bombesin conjugates to target breast and prostate cancer cells. The novel idea of adding the Tat (49-57) peptide to the radiopharmaceutical in order to penetrate the cell nucleus is a new proposal for therapy at cellular level. {sup 99m}Tc radionuclide produces Auger energy of 0.9 keV/decay and internal conversion electron energy of 15.4 keV/decay, which represent 11.4% of the total {sup 99m}Tc energy released per decay. It is expected that the dose delivered at specific microscopic levels in cancer cells induce a therapeutic effect. The aim of this research was to assess in vitro internalization kinetics in breast and prostate cancer cells of {sup 99m}Tc-Tat(49-57)-bombesin and to evaluate the radiation absorbed dose at subcellular level simulating the electron transport. The pen main program from the 2006 version of the Penelope code was used to simulate and calculate the absorbed dose by Auger and internal conversion electron contribution in the membrane, cytoplasm and nucleus of Pc-3 prostate cancer and MCF7 and MDA human breast cancer cell lines. Nuclear data were obtained from the 2002 BNM-LNHB {sup 99m}Tc decay scheme. The spatial distribution of the absorbed doses to the membrane, cytoplasm and nucleus were calculated using a geometric model built from real images of cancer cells. The elemental cell composition was taken from the literature. The biokinetic data were obtained evaluating total disintegrations in each subcellular compartment by integration of the time-activity curves acquired from experimental data. Results showed that 61, 63 and 46% of total disintegrations per cell-bound {sup 99m}Tc-Tat-Bn activity unit occurred in the nucleus of Pc-3, MCF7 and MDA-MB231 respectively. {sup 99m}Tc--Tat-Bn absorbed doses were 1.78, 5.76 and 2.59 Gy/Bq in the nucleus of

  9. Clinical investigation of accumulation process of {sup 99m}Tc-HMDP and -MDP in bone

    Energy Technology Data Exchange (ETDEWEB)

    Matumoto, Takahiro [Health Insurance Naruto Hospital, Tokushima (Japan); Miyoshi, Hirokazu; Satou, Kazuo; Kishi, Taro; Yoshino, Tomio; Nishitani, Hiromu

    2000-06-01

    The accumulation process in bone of {sup 99m}Tc-hydroxymethylene diphosphonate ({sup 99m}Tc-HMDP) and {sup 99m}Tc-methylene diphosphonate ({sup 99m}Tc-MDP) was examined on 9 subjects (5 for {sup 99m}Tc-HMDP and 4 for {sup 99m}Tc-MDP). {sup 99m}Tc compounds in blood were analyzed with radio-thinlayer chromatography, radioactivity was measured using a scintillation counter, and the uptake of both {sup 99m}Tc diphosphonates in bone was examined by scintigraphy. The amounts of {sup 99m}Tc-HMDP and MDP compounds disappeared from blood for 2 h after injection were 83% and 87%, respectively, and these were almost unchanged even after 3 h. While the greater part of {sup 99m}TcO{sub 4}{sup -} contained in both diphosphonates was disappeared from blood, the small amounts of unknown {sup 99m}Tc compounds were produced in blood. The ratio of the radioactivity of {sup 99m}Tc-HMDP in lumbar vertebra to soft tissue near bone was 1.5{+-}0.5 after about 3 h, showing that equilibrium occurred in the process of the accumulation in bone, and good scintigrams were depicted in all the cases. These results suggest that the accumulation in bone of both {sup 99m}Tc diphosphonates may be by both fast chemical absorption onto the surface of the hydroxyapatite in bone and slow uptake mediated through osteoblast-like cells and organic matters. (author)

  10. Guarana (Paullinia cupana) effect on radiolabelling of erythrocytes with Technetium-99m; Efeito do guarana (Paulinnia cupana) na marcacao de hemaceas com tecnecio-99m

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Joelma F.; Braga, Ana Cristina S.; Avila, Antonio Sergio R.; Gutfilen, Bianca [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Biologia; Bernardo-Filho, Mario [Instituto Nacional do Cancer, Rio de Janeiro, RJ (Brazil)

    1996-07-01

    The labelling of red blood cells (RBC) with technetium-99m (Tc-99m) have been used for many studies in nuclear medicine. This labeling procedure depends on a reducing agent and stannous chloride is normally used for this purpose. Many factors, including drug therapy, can affect the biodistribution of radiopharmaceuticals, such factors many lead to poor organ visualization, requirement to repeat the examination procedure or even misdiagnosis. Guarana (Paullinia cupana) is commonly used in popular medicine. Here, we investigate if guarana is capable to alter the labeling of RBC with Tc-99m and to modify the radioactivity binding to plasma proteins and cellular constituents from insoluble and soluble fractions. The results were obtained with blood samples from Wistar rats with heparine and incubated with different guarana solutions. The percentage of radioactivity (% rad) from BC and P were calculated. The distribution of radioactivity in P and BC showed that the uptake of Tc-99m decreased significantly in all concentrations. (author)

  11. Characterization of technetium-99m-L,L-ECD for brain perfusion imaging, Part 1: Pharmacology of technetium-99m ECD in nonhuman primates

    Energy Technology Data Exchange (ETDEWEB)

    Walovitch, R.C.; Hill, T.C.; Garrity, S.T.; Cheesman, E.H.; Burgess, B.A.; O' Leary, D.H.; Watson, A.D.; Ganey, M.V.; Morgan, R.A.; Williams, S.J. (E.I. Du Pont de Nemours Co., Inc., No. Billerica, MA (USA))

    1989-11-01

    Technetium-99m ethyl cysteinate dimer (({sup 99m}Tc)ECD) is a neutral, lipophilic complex which rapidly crosses the blood-brain barrier. Brain retention and tissue metabolism of ({sup 99m}Tc)ECD is dependent upon the stereochemical configuration of the complex. While both L,L and D,D enantiomers are extracted by the brain, only the L,L but not the D,D form, is metabolized and retained in the monkey brain (4.7% injected dose initially, T 1/2 greater than 24 hr). Dynamic single photon emission computed tomography imaging studies in one monkey indicates {sup 99m}Tc-L,L-ECD to be distributed in a pattern consistent with regional cerebral blood flow for up to 16 hr postinjection. Dual-labeled {sup 99m}Tc-L,L-ECD and ({sup 14}C)iodoantipyrine autoradiography studies performed 1 hr after administration show cortical gray to white matter ratios of both isotopes to be equivalent (approximately 4-5:1). These data suggest that {sup 99m}Tc-L,L-ECD will be useful for the scintigraphic assessment of cerebral perfusion in humans.

  12. sup(99m)Tc-DTPA and sup(99m)Tc-DMSA renal gamma imaging in the surveillance of patients with conduit urinary diversion

    Energy Technology Data Exchange (ETDEWEB)

    Lindell, O.; Lehtonen, T.; Kivisaari, A.

    1986-06-01

    We studied renal anatomy and function using sup(99m)Tc-2-3 dimercaptosuccinic acid (DMSA) and sup(99m)Tc-diethylenetriamine pentaacetic acid (DTPA) in 27 patients with conduit urinary diversion. In this condition, free ureteral reflux is often associated with bacteriuria, and these factors are thought to precipitate progressive renal deterioration. Gamma-camera images provided valuable information concerning the structure of the renal parenchyma, the function of individual kidneys and possible ureteral obstruction, thus helping us to decide whether or not to instigate further treatment. The information gained using renal gamma imaging with sup(99m)Tc-DTPA and sup(99m)Tc-DMSA was complementary and partly overlapping. We preferred the use of sup(99m)Tc-DTPA because of its ability to visualise the ureters and the region of ureteroconduit anastomosis. Using diuretic medication, we were able to differentiate true ureteral obstruction from atony in 9 patients using sup(99m)Tc-DTPA.

  13. Diagnosis of Raynaud's phenomenon by (99m)Tc-pertechnetate hand perfusion scintigraphy: a pilot study.

    Science.gov (United States)

    Pavlov-Dolijanovic, Slavica; Petrovic, Nebojsa; Vujasinovic Stupar, Nada; Damjanov, Nemanja; Radunovic, Goran; Babic, Dragan; Sobic-Saranovic, Dragana; Artiko, Vera

    2016-12-01

    We assess the usefulness of (99m)Tc-pertechnetate hand perfusion scintigraphy in patients with Raynaud's phenomenon (RP). The study population consisted of 18 patients with primary RP, 25 patients with secondary RP within systemic sclerosis (SSc), and ten healthy individuals. Gamma camera dynamic first-pass study during the first 60 s and a static scintigraphy after 5 min were recorded following a bolus injection of (99m)Tc-pertechnetate via a cubital vein. Regions of interest were drawn on the summed images around the fingers and the palmar region. The fingers-to-palm ratios were then calculated. The mean fingers-to-palm ratio for dynamic study (blood flow) was 0.58 ± 0.19 for the healthy group, 0.45 ± 0.18 for the primary RP, and 0.43 ± 0.21 for the SSc patients. The mean fingers-to-palm ratio for static study (blood pool) was 0.44 ± 0.06 for the healthy group, 0.42 ± 0.06 for the primary RP, and 0.36 ± 0.07 for the SSc patients. Analysis of variance showed these differences to be significant (p = 0.039 from blood flow and p = 0.004 from blood pool). The receiver operating characteristic curve showed sensitivity of 80% and a specificity of 60% when using cutoff values of 0.40 for blood flow and sensitivity of 79% and a specificity of 70% when using cutoff values of 0.37 for blood pool. Our method is able to differentiate between patients with normal and those with abnormal microcirculation of the hands. Dynamic study separates the healthy subjects from patients with RP, while static study separates primary from secondary RP.

  14. Comparative microvascular exchange kinetics of [{sup 77}Br]bromide and {sup 99m}Tc-DTPA in humans

    Energy Technology Data Exchange (ETDEWEB)

    Cousins, Claire [Department of Clinical Radiology, Addenbrooke' s Hospital, Cambridge (United Kingdom); Skehan, Stephen J.; Rolph, Susan M.; Flaxman, Mary E.; Ballinger, James R.; Bird, Nicholas J.; Barber, Robert W.; Peters, Michael A. [Department of Nuclear Medicine, Addenbrooke' s Hospital, Cambridge (United Kingdom)

    2002-05-01

    The plasma clearance curves of small hydrophilic solutes comprise three exponentials, consistent with a three-compartmental distribution model. A previous comparison between inulin and diethylene triamine penta-acetic acid (DTPA) suggested that these three compartments are in series, the first being plasma and the second and third representing compartments within the extravascular space. Moreover, whilst the total distribution volumes of these two indicators were similar, the volume of the second compartment was higher for DTPA. The purpose of the current study was to investigate whether a solute smaller than DTPA, namely bromide, fits the hypothesis that the second space volume is an inverse function of the size of the solute. Two groups of subjects were studied: group A comprised eight patients undergoing routine diagnostic arteriography and group B, eight patients referred for routine measurement of glomerular filtration rate plus two normal volunteers. {sup 99m}Tc-DTPA and sodium [{sup 77}Br]bromide were intravenously administered simultaneously. In group A, frequent arterial samples were obtained up to 40 min after injection, and antecubital venous samples 30 s after each arterial sample. In group B, frequent venous samples were obtained up to 280 min after injection. Volume measurements based on bromide were corrected for erythrocyte bromide accumulation. In both subject groups, the normalised venous concentration ratio of bromide to DTPA, corrected for red cell bromide uptake, was significantly less than unity in the earliest blood samples, being 0.56 (SD 0.08) at 1 min, consistent with faster diffusion of bromide from plasma to interstitial fluid. Furthermore, the extraction fraction of bromide from plasma to interstitial fluid in the forearm was about 0.6, higher than that of DTPA (about 0.5) in spite of red cell bromide accumulation which equilibrated with plasma bromide within 20 s and resulted in a red cell to plasma concentration ratio of 0.51 (0

  15. Comparative study of 188Re( V )-DMSA and 99mTc ( V )-DMSA in tumor model%188Re(V)-DMSA与99mTc(V)-DMSA在肿瘤模型体内的对比研究

    Institute of Scientific and Technical Information of China (English)

    孙逊; 安锐

    2004-01-01

    Objective To compare the biodistribution and imaging characteristics of 188Re(V)-DMSA and 99mTc(V)-DMSA in tumor model, and to discuss the possibility of treating tumors with 188Re(V)-DMSA. Methods The solid neoplasm bearing mice (Ehrlich carcinoma bearing mice) models underwent biodistribution study and static whole body planar imaging after injection of 188Re(V)-DMSA and 99mTc(V)-DMSA respectively. When the mice were sacrificed at different time after the injection, the tumor, blood and contralateral normai muscles were removed, weighted and the radioactivity was measured. Then the radioactivity ratios of target (tumor)-to-blood (T/B),target-to-non targeted (contralateral limbs or muscles) (T/NT) were calculated. ROIs were drawn and T/NT were calculated in planar imagines. Results Two radiopharmaceuticals were mainly concentrated in bone and kidney, and the uptake ratios in tumor were high too.The half-clearance times of these two radiopharmaceuticals in blood were both less than 1h. The greatest T/NT ratio of 99mTc group was higher than 188Re group in planar imagings, but the highest T/B, T/NT ratios of these two radiopharmaceuticals in biodistribution study had no significant difference and were all above 3.0. Conclusion The biodistribution characteristics of 188Re(V)-DMSA and 99mTc( V)-DMSA were similar. 188Re(V)-DMSA has good applied foreground in treating tumors and their metastases.%目的比较188Re(V)-DMSA(五价188Re-二巯基丁二酸钠)和99mTc(V)-DMSA在肿瘤模型体内生物分布与显像的特点,探讨188Re(V)-DMSA用于肿瘤治疗的可能性.方法用188Re(V)-DMSA和99mTc(V)-DMSA对实验性实体肿瘤(小鼠艾氏腹水癌)模型进行生物学分布实验和全身平面显像,并通过脏器克组织百分摄取率(%ID/g)测定法和感兴趣区(ROI)技术进行定量分析,计算各时点两种放射性药物的靶/血、靶/非靶比值.结果两种放射性药物均主要浓聚于骨骼和肾脏,肿瘤组织也有较高的摄

  16. Dimer of the peptide cycle (Ar-Gly-Asp-D-Phe-Lys) radiolabeled with {sup 99m}Tc for the integrin s over-expression image: formulation, biokinetics and dosimetry; Dimero del peptido ciclo(Arg-Gly-Asp-D-Phe-Lys) radiomarcado con {sup 99m}Tc para la imagen de sobre-expresion de integrinas: formulacion, biocinetica y dosimetria

    Energy Technology Data Exchange (ETDEWEB)

    Ortiz A, Z.

    2013-07-01

    In breast cancer, α(v)β(3) and/or α(v)β(5) integrin s are over-expressed in both endothelial and tumour cells. Radiolabeled peptides based on the RGD (Arg-Gly-Asp) sequence are radiopharmaceuticals with high affinity and selectivity for those integrin s. The RGD-dimer peptide (E-[c(RGDfK)]{sub 2}) radiolabeled with {sup 99m}Tc has been reported as a radiopharmaceutical with 10-fold higher affinity for the α(v)β(3) integrin as compared to the RGD-monomer. EDDA (Ethylenediamine-N,N-diacetic acid) is a hydrophilic molecule that may favours renal excretion when used as coligand in the {sup 99m}Tc labelling of HYNIC-peptides and can easily be formulated in a lyophilized kit. Aim: Establish a biokinetic model for {sup 99m}Tc-EDDA/HYNIC-E-[c(RGDfK)]{sub 2} prepared from lyophilized kits and evaluate the dosimetry as breast cancer imaging agent. Methods: {sup 99m}Tc labelling was performed by addition of sodium pertechnetate solution and 0.2 M phosphate buffer ph 7.0 to a lyophilized formulation containing E-[c(RGDfK)]{sub 2}, EDDA, tricine, mannitol and stannous chloride. Radiochemical purity was evaluated by reversed phase HPLC and ITLC-SG analyses. Stability studies in human serum were carried out by size-exclusion HPLC. In-vitro cell uptake was tested using breast cancer cells (MCF7, T47D and MDA-MB-231) with blocked and non-blocked receptors. Biodistribution and tumour uptake were determined in MCF7 tumour-bearing nude mice with blocked and non-blocked receptors, and images were obtained using a micro-SPECT/CT. Whole-body images from seven healthy women were acquired at 1, 3, 6 and 24 h after {sup 99m}Tc-EDDA/HYNIC-E-[c(RGDfK)]{sub 2} administration obtained with radiochemical purities of >94 %. Regions of interest (ROIs) were drawn around source organs on each time frame. Each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate {sup 99m}Tc-EDDA/HYNIC-E-[c(RGDfK)]{sub 2} time-activity curves in each

  17. Usefulness of per-rectal portal scintigraphy with Tc-99m pertechnetate for galactosemia in infants

    Energy Technology Data Exchange (ETDEWEB)

    Shiomi, Susumu; Sasaki, Nobumitsu; Ikeoka, Naoko; Kuroki, Tetsuo; Okano, Yoshiyuki; Kawabe, Joji; Ochi, Hironobu [Osaka City Univ. (Japan). Medical School

    1998-12-01

    Galactosemia discovered by newborn screening is rarely caused by enzyme deficiency. It has recently been reported that among patients without enzyme deficiency portosystemic shunting may be a cause of galactosemia in some patients. We did per-rectal portal scintigraphy in patients with such galactosemia detected during screening of newborns to examine the usefulness of this method for the diagnosis of portosystemic shunts via the inferior mesenteric vein. The subjects were eight neonates with galactosemia without enzyme deficiency detected during screening. A solution containing technetium-99m pertechnetate was instilled into the rectum, and serial scintigrams were taken while radioactivity curves for the liver and heart were recorded sequentially. The per-rectal portal shunt index was determined by calculating the ratio for counts of the liver to counts for the heart integrated for 24 seconds immediately after the appearance of the liver time-activity curve. A portosystemic shunt was detected in both of the patients with a shunt index of 30% or more, but not in the six patients with a shunt index less than 30%. The blood galactose levels of these six patients later entered the reference range. This method is noninvasive and there is little exposure to the radionuclide. It seemed to be useful for the diagnosis of portosystemic shunt in newborns with galactosemia without enzyme deficiency. (author)

  18. Serum Human Chorionic Gonadotropin (β- hCG) Clearance Curves in Women with Successfully Expectantly Managed Tubal Ectopic Pregnancies: A Retrospective Cohort Study.

    Science.gov (United States)

    Helmy, Samir; Mavrelos, Dimitrios; Sawyer, Elinor; Ben-Nagi, Jara; Koch, Marianne; Day, Andrea; Jurkovic, Davor

    2015-01-01

    To establish clearance curves for serum β -hCG in women with successfully expectantly managed tubal ectopic pregnancies. Retrospective cohort study. Non- viable tubal ectopic pregnancy was diagnosed on transvaginal ultrasound. If initial serum β hCG was less than 5000 IU/L and patients were asymptomatic, expectant management was offered. Patients underwent serial β hCG measurements until serum β hCG was less than 20 IU/l, or the urine pregnancy test was negative. Early Pregnancy and Gynaecology Assessment Unit, Kings College Hospital, London (December 1998 to July 2006). We included 161 women with diagnosed non-viable tubal ectopic pregnancy who underwent successful expectant management. Serum β hCG level. Mean initial serum β- hCG was 488 IU/L (41 - 4883) and median serum β hCG clearance time was 19 days (5 - 82). The average half-life of β hCG clearance was 82.5 hours (±SD 50.2) in patients with steadily declining serum β- hCG levels compared to 106.7 hours (±SD 72.0) in patients with primarily plateauing β-hCG levels in the declining phase. However, these differences were not significant (p>0.05). We identified a median follow-up of 19 days until serum β hCG clearance in women with tubal ectopic pregnancy and successful expectant management. Although non- significant, women with initially plateauing serum β hCG showed a longer follow-up time until clearance compared to women with steadily declining β hCG levels. This information may serve as a guideline enabling clinicians to predict the length of follow-up for women with tubal ectopic pregnancy and expectant management.

  19. {sup 99m}Tc-2GAM: A tracer for renal imaging

    Energy Technology Data Exchange (ETDEWEB)

    Gianolli, Luigi; Dosio, Flaviano; Matarrese, Mario; Colombo, Fabio; Cutler, Cathy; Stepniak-Biniakiewicz, Danuta; Deutsch, Edward; Savi, Annarita; Lucignani, Giovanni; Fazio, Ferruccio

    1996-11-01

    We propose a renal imaging agent, the {sup 99m}Tc complex of the bidentate-N,S chelate N-(mercaptoacetyl)glycine ({sup 99m}Tc-2GAM), with the imaging characteristics of {sup 99m}Tc-DMSA but a faster kidney uptake; chemical evidence supports the formulation of {sup 99m}Tc-2GAM as [Tc{sup V}(O)(GAM){sub 2}]{sup -}. After biodistribution and toxicity studies in animals, {sup 99m}Tc-2GAM was evaluated in five normal volunteers. {sup 99m}Tc-2GAM is rapidly cleared from the blood (t{sub (1(2))} = 9 min) and 50% of the ID is excreted in the urine in the first 2 h. Dynamic data show a rapid renal uptake that increases up to 1 h with no significant wash-out between 1 and 8 h. The uptake in each kidney ranges from 11.3% to 20.7% ID. Low, stable liver uptake is observed. No significant activity is detected in other organs. We showed no differences between {sup 99m}Tc-2GAM and {sup 99m}Tc-DMSA compared in three patients with unilateral kidney disease. We conclude that {sup 99m}Tc-2GAM has good practical and dosimetric features for renal imaging.

  20. Primary neuroblastoma uptake of /sub 99m/technetium methylene diphosphonate

    Energy Technology Data Exchange (ETDEWEB)

    Smith, F.W.; Gilday, D.L.; Ash, J.M.; Reid, R.H.

    1980-11-01

    Forty patients, ages three days to 12 years, with neuroblastomas had bone scans with /sub 99m/technetium methylene diphosphonate (/sub 99m/Tc-MDP) as part of their pretreatment examination. Twenty-four (60%) had primary tumor uptake and 16 (40%) did not. No difference was seen between the two groups in the incidence of tumor calcification or necrosis. No relationship between the level of urinary vanillylmandelic acid (VMA) and the presence of primary tumor uptake of /sub 99m/Tc-MDP was found. The possible causes for the localization of /sub 99m/Tc-MDP are discussed.

  1. {sup 99m}Tc reduces clonogenic survival after intracellular uptake in NIS positive cells in vitro more than {sup 131}I; {sup 99m}Tc reduziert nach intrazellulaerer Aufnahme in NIS-positiven Zellen in vitro das klonogene Ueberleben staerker als {sup 131}I

    Energy Technology Data Exchange (ETDEWEB)

    Wendisch, M.; Freudenberg, R.; Drechsel, J.; Runge, R.; Wunderlich, G.; Kotzerke, Joerg [Universitaetsklinikum Carl Gustav Carus Dresden - Technische Univ. Dresden (Germany). Klinik und Poliklinik fuer Nuklearmedizin

    2010-07-01

    In addition to gamma radiation of 140 keV {sup 99m}Tc emits during the transition to {sup 99}Tc electrons of low energy and tiny path-lengths. These Auger electrons cannot be utilized in diagnostic procedures. However, they were discussed frequently for therapeutic application. Hitherto proof of effect of the Auger electrons from {sup 99m}Tc is missing which is supplied now in an in vitro-system in comparison to beta-emitter {sup 131}I. The thyroid cell line PC CI3 (sodium iodide symporter (NIS)-positive) was incubated with {sup 131}I-sodium iodide ({sup 131}I) or {sup 99m}Tc-pertechnetate ({sup 99m}Tc) in presence or absence of perchlorate. For comparison the amount of radioactivity was adjusted to obtain the same dose from extracellular irradiation for both radionuclides. The colony forming assay detects the clonogenic cell survival as surviving fraction. In addition, intracellular radionuclide uptake was quantified. Dose effect curves were established for {sup 131}I and {sup 99m}Tc for variable extra- and intracellular distribution of the radioactivity. In presence of perchlorate no cellular uptake of radioactivity was detectable. Survival curves were largely comparable confirming the dosimetric calculations. In absence of perchlorate cellular radiotracer uptake varied from 1.39% ({sup 131}I) to 1.90% ({sup 99m}Tc). Effects on survival were twice for the beta-emitter and ten-fold higher for {sup 99m}Tc. Intracellular uptake of {sup 131}I and {sup 99m}Tc increases DNA-damage compared to strict extracellular radiotracer distribution which was demonstrated by means of colony forming assay. Increasing radiotoxicity from intracellular {sup 99m}Tc is explained most likely by increased dose deposition in cellular structures due to Auger- und conversion-electrons of low range and high local energy deposition. (orig.)

  2. Effective detection of the tumors causing osteomalacia using [Tc-99m]-HYNIC-octreotide (99mTc-HYNIC-TOC) whole body scan

    Energy Technology Data Exchange (ETDEWEB)

    Jing, Hongli, E-mail: annsmile1976@sina.com [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Li, Fang, E-mail: lifang@pumch.cn [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Zhuang, Hongming, E-mail: zhuang@email.chop.edu [Department of Radiology, The Children' s Hospital of Philadelphia, University of Pennsylvania Perelman School of Medicine, 34th and Civic Ctr Blvd, Philadelphia, PA 19104 (United States); Wang, Zhenghua, E-mail: ccq1214@yahoo.com.cn [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Tian, Jian, E-mail: tianjian4809@hotmail.com [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Xing, Xiaoping, E-mail: xingxp@126.com [Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Jin, Jin, E-mail: jinjin9010@126.com [Department of Orthopedic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Zhong, Dingrong, E-mail: ZhongDR@pumch.cn [Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Zhang, Jingjing, E-mail: zhangjingjingtag@163.com [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China)

    2013-11-01

    Purpose: Tumor-induced osteomalacia (TIO) is an endocrine disorder caused by tumors producing excessive fibroblast growth factor-23 (FGF-23). The causative tumors are generally small, slow-growing benign mesenchymal tumors. The only cure of the disease depends on resection of the tumors, which are extremely difficult to localize due to their small sizes and rare locations. Since these tumors are known to express somatostatin receptors, this research was undertaken to evaluate efficacy of [Tc-99m]-HYNIC-octreotide (99mTc-HYNIC-TOC) whole body imaging in this clinical setting Methods: Images of 99mTc-HYNIC-TOC scans and clinical chart from 183 patients with hypophosphatemia and clinically suspected TIO were retrospectively reviewed. The scan findings were compared to the results of histopathological examinations and clinical follow-ups. Results: Among 183 patients, 72 were confirmed to have TIO while 103 patients were found to have other causes of hypophosphatemia. The possibility of TIO could not be either diagnosed or excluded in the remaining 8 patients. For analytical purposes, these 8 patients who could neither be diagnosed nor excluded as having TIO were regarded as having the disease, bringing the total of TIO patients to 80. The 99mTc-HYNIC-TOC scan identified 69 tumors in 80 patients with TIO, which rendered a sensitivity of 86.3% (69/80). 99mTc-HYNIC-TOC scintigraphy excluded 102 patients without TIO with a specificity of 99.1% (102/103). The overall accuracy of 99mTc-HYNIC-TOC whole body scan in the localization of tumors responsible for osteomalacia is 93.4% (171/183). Conclusions: Whole body 99mTc-HYNIC-TOC imaging is effective in the localization of occult tumors causing TIO.

  3. The role of Tc-99m DTPA aerosol scintigraphy in the differential diagnosis of COPD and asthma.

    Science.gov (United States)

    Karacavus, Seyhan; Intepe, Yavuz S

    2015-04-01

    Chronic obstructive lung disease (COPD) and asthma are characterized as similar to each other in causing airway obstruction and being an inflammatory process. The purpose of this study was to investigate whether technetium-99m diethylenetriaminepentaacetic acid ((99m) Tc-DTPA) aerosol scintigraphy could be used in the differential diagnosis of asthma and COPD. Eighty-four patients (male/female: 32/52; mean age 50.2 ± 12.7 years) with obstructive lung disease and 30 healthy volunteers as the control group were enrolled in the study. The patients were divided into two groups as COPD and asthma and also smoking subgroups. Alveolar clearance study was performed using a radiolabeled aerosol of (99m) Tc-DTPA. Mucociliary clearance was evaluated with T½ , cap value and penetration index parameters. All patient underwent pulmonary function tests and Forced expiratory volume (FEV1 ), forced vital capacity (FVC) and FEV1 /FVC parameters were obtained. The mean of T½ values of (99m) Tc-DTPA aerosol and FEV1 /FVC value among spirometric tests of the nonsmoking COPD patients were significantly lower than nonsmoking asthma patients (46.1 ± 14.3, 62.3 ± 18.7, P = 0.02; 65.2 ± 10.8, 81.4 ± 16.5, P = 0.04, respectively). The cap value was significantly higher in nonsmoking COPD patients (1.21 ± 0.49, 0.76 ± 0.22, P = 0.03). While there were no statistically and significantly different between control and asthmatic groups at the scintigraphic parameters and spirometric parameters, the mean of T½ values, cap value and spirometric parameters were statistically different between control and COPD groups (P scintigraphy was a useful, easy to apply and a noninvasive technique to use in the differential diagnosis of nonsmoker COPD and asthma. © 2014 John Wiley & Sons Ltd.

  4. 99mTcO-4/99mTc-MIBI联合显像对甲状腺癌的诊断价值%Diagnosis value of 99mTcO-4/99mTc-MIBI combined with imaging in thyroid carcinoma

    Institute of Scientific and Technical Information of China (English)

    孙文伟; 魏丽琴; 侯洁; 任磊; 戚良晨

    2010-01-01

    目的:比较99mTcO-4/99mTc-甲氧基异丁基异腈(99mTcO-4/99mTc-MIBI)联合显像与颈部彩色多普勒超声(CDU)检查在甲状腺癌、术后甲状腺癌复发或颈部转移中的诊断结果,探讨99mTcO-4/99mTc-MIBI联合显像在甲状腺癌诊断中的价值.方法:选择经手术治疗甲状腺占位患者150例以及甲状腺癌术后疑似复发或颈部转移而行二次手术患者21例.以术后病理结果为金标准,分别对99mTcO-4/99mTc-MIBI联合显像、颈部CDU检查在437个甲状腺占位、215个直径大于1 cm的甲状腺占位以及87个疑似甲状腺癌术后复发或颈部转移中的诊断结果进行对比分析.结果:在甲状腺癌的诊断中CDU检查灵敏度(89.10%)高于99mTcO-4/99mTc-MIBI联合显像(81.99%);CDU与99mTcO-4/99mTc-MIBI联合显像的病理符合率分别为84.67%和80.78%,差异无显著性(P>0.05).在直径大于1 cm甲状腺癌的诊断中99mTcO-4/99mTc-MIBI联合显像灵敏度(97.75%)高于CDU检查(89.89%);CDU与99mTcO-4/99mTc-MIBI联合显像的病理符合率分别为84.65%和87.44%,差异无显著性(P>0.05).在术后甲状腺癌复发或颈部转移灶的诊断中颈部CDU检查灵敏度、特异性(44.78%,40.00%)均低于99mTcO-4/99mTc-MIBI联合显像(64.18%,80.00%);CDU与99mTcO-4/99mTc-MIBI联合显像的病理符合率分别为43.68%和67.82%,差异有显著性(P<0.05).结论:99mTcO-4/99mTc-MIBI联合显像对直径较大的甲状腺癌诊断较灵敏,尤其是在诊断术后甲状腺癌复发或颈部转移方面优势明显.

  5. 99mTc-labeled HYNIC-DAPI causes plasmid DNA damage with high efficiency.

    Science.gov (United States)

    Kotzerke, Joerg; Punzet, Robert; Runge, Roswitha; Ferl, Sandra; Oehme, Liane; Wunderlich, Gerd; Freudenberg, Robert

    2014-01-01

    (99m)Tc is the standard radionuclide used for nuclear medicine imaging. In addition to gamma irradiation, (99m)Tc emits low-energy Auger and conversion electrons that deposit their energy within nanometers of the decay site. To study the potential for DNA damage, direct DNA binding is required. Plasmid DNA enables the investigation of the unprotected interactions between molecules and DNA that result in single-strand breaks (SSBs) or double-strand breaks (DSBs); the resulting DNA fragments can be separated by gel electrophoresis and quantified by fluorescent staining. This study aimed to compare the plasmid DNA damage potential of a (99m)Tc-labeled HYNIC-DAPI compound with that of (99m)Tc pertechnetate ((99m)TcO4(-)). pUC19 plasmid DNA was irradiated for 2 or 24 hours. Direct and radical-induced DNA damage were evaluated in the presence or absence of the radical scavenger DMSO. For both compounds, an increase in applied activity enhanced plasmid DNA damage, which was evidenced by an increase in the open circular and linear DNA fractions and a reduction in the supercoiled DNA fraction. The number of SSBs elicited by 99mTc-HYNIC-DAPI (1.03) was twice that caused by (99m)TcO4(-) (0.51), and the number of DSBs increased fivefold in the (99m)Tc-HYNIC-DAPI-treated sample compared with the (99m)TcO4(-) treated sample (0.02 to 0.10). In the presence of DMSO, the numbers of SSBs and DSBs decreased to 0.03 and 0.00, respectively, in the (99m)TcO4(-) treated samples, whereas the numbers of SSBs and DSBs were slightly reduced to 0.95 and 0.06, respectively, in the (99m)Tc-HYNIC-DAPI-treated samples. These results indicated that (99m)Tc-HYNIC-DAPI induced SSBs and DSBs via a direct interaction of the (99m)Tc-labeled compound with DNA. In contrast to these results, (99m)TcO4(-) induced SSBs via radical formation, and DSBs were formed by two nearby SSBs. The biological effectiveness of (99m)Tc-HYNIC-DAPI increased by approximately 4-fold in terms of inducing SSBs and by

  6. 99mTc-labeled HYNIC-DAPI causes plasmid DNA damage with high efficiency.

    Directory of Open Access Journals (Sweden)

    Joerg Kotzerke

    Full Text Available (99mTc is the standard radionuclide used for nuclear medicine imaging. In addition to gamma irradiation, (99mTc emits low-energy Auger and conversion electrons that deposit their energy within nanometers of the decay site. To study the potential for DNA damage, direct DNA binding is required. Plasmid DNA enables the investigation of the unprotected interactions between molecules and DNA that result in single-strand breaks (SSBs or double-strand breaks (DSBs; the resulting DNA fragments can be separated by gel electrophoresis and quantified by fluorescent staining. This study aimed to compare the plasmid DNA damage potential of a (99mTc-labeled HYNIC-DAPI compound with that of (99mTc pertechnetate ((99mTcO4(-. pUC19 plasmid DNA was irradiated for 2 or 24 hours. Direct and radical-induced DNA damage were evaluated in the presence or absence of the radical scavenger DMSO. For both compounds, an increase in applied activity enhanced plasmid DNA damage, which was evidenced by an increase in the open circular and linear DNA fractions and a reduction in the supercoiled DNA fraction. The number of SSBs elicited by 99mTc-HYNIC-DAPI (1.03 was twice that caused by (99mTcO4(- (0.51, and the number of DSBs increased fivefold in the (99mTc-HYNIC-DAPI-treated sample compared with the (99mTcO4(- treated sample (0.02 to 0.10. In the presence of DMSO, the numbers of SSBs and DSBs decreased to 0.03 and 0.00, respectively, in the (99mTcO4(- treated samples, whereas the numbers of SSBs and DSBs were slightly reduced to 0.95 and 0.06, respectively, in the (99mTc-HYNIC-DAPI-treated samples. These results indicated that (99mTc-HYNIC-DAPI induced SSBs and DSBs via a direct interaction of the (99mTc-labeled compound with DNA. In contrast to these results, (99mTcO4(- induced SSBs via radical formation, and DSBs were formed by two nearby SSBs. The biological effectiveness of (99mTc-HYNIC-DAPI increased by approximately 4-fold in terms of inducing SSBs and by approximately

  7. In vitro properties and in vivo behavior of technetium-99m labeled fibrinogens

    Energy Technology Data Exchange (ETDEWEB)

    Karube, Yoshiharu; Ito, Sanae; Katsuno, Kentaro; Takata, Jiro [Fukuoka Univ. (Japan). Faculty of Pharmaceutical Sciences

    1996-02-01

    Fibrinogen was labeled with Tc-99m by two methods and the in vitro stability and in vivo behavior in mice were studied. The Tc-99m labeling was performed by mixing an unreduced fibrinogen (UnFib) or a reduced fibrinogen (ReFib) with Tc-99m pertechnetate in the presence of stannous chloride. In both of them, chelation with Tc-99m resulted in a single radiochemical product. For the in vitro stability studies, Tc-99m labeled fibrinogen (Tc-99m UnFib) was prepared with UnFib, and transchelation with cysteine solution was easy to produce compared to Tc-99m labeled fibrinogen (Tc-99m ReFib) prepared with ReFib. The radioactivity bound to clottable protein for Tc-99m UnFib and Tc-99m ReFib was about 70% and about 69%, respectively. The in vivo behavior of these labeled fibrinogens was studied, and their efficiencies for imaging an abscess and Ehrlich tumor in mice were determined with a gamma camera. Technetium-99m UnFib underwent a rapid partial exchange of the Tc-99m with compounds of the blood buffer system in vivo, resulting in early urinary excretion. On the other hand, the fraction of Tc-99m ReFib that remained intact in vivo was biologically active and would be incorporated into the abscess and tumor. The uptake in the abscess increased slightly over time with Tc-99m ReFib, but the abscess to blood and abscess to muscle ratios were 0.09 and 2.6 at 5 hr, respectively. Clearly delineated images of the abscess were obtained beginning at about 5 hr after injection. The tumor to blood and tumor to muscle ratios were 0.05 and 1.4 at 5 hr, respectively. The Ehrlich tumor image in mice was slightly visible at 10 hr. The short half-life of Tc-99m was inappropriate for fibrinogen with a low pharmacokinetic value, because it was necessary for imaging of the abscess and tumor to take a long time. (author).

  8. The Use of ~(99m)Tc-Scanning in Children with Acute Abdomen%~(99m)锝在小儿急腹症的选择应用

    Institute of Scientific and Technical Information of China (English)

    刘唐彬; 李伟龙; 李穗生; 莫家骢

    1988-01-01

    ~(99m)Tc-scanning is used extensively in the examination of the thyroid gland,salivary glands and stomach. The affinity of this isotope to the parietal cells of the gastric mucosa makes it useful in detecting the ectoic gastric mucosa.~(99m)Te has a half-life period of only six hours and is used in blood pool studies.Seven children with acute abdomen were examined with ~(99m)Tc-scanning,5 of them were positive,Discussed in the article are: (1) the general indication;(2)its use in acute gastrointestinal bleeding;(3)the diagnosis of Meckel's diverticulum and (4)the screening study of recurrent vomiting and abdominal pain.%文章利用~(99m)锝半衰期短,主要经胃粘膜壁细胞排泄,及可作为血池显像剂特点,对7例急腹症小儿进行筛选检查,核素诊断阳性者5例.指出~(99m)锝腹部扫描,不仅有助于美克耳氏憩室出血的诊断,也可作为鉴别消化道出血的一个方法,对反复腹痛、呕吐患儿的病因筛选检查研究具有一定的临床价值.

  9. Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [{sup 99m}Tc(MIBI){sub 6}]{sup +}

    Energy Technology Data Exchange (ETDEWEB)

    Faria, D.P.; Buchpiguel, C.A.; Marques, F.L.N., E-mail: danielefaria1@gmail.com [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Faculdade de Medicina. Departamento de Radiologia. Servico de Medicina Nuclear

    2015-10-15

    Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [{sup 99m}Tc(MIBI){sub 6}]{sup +} as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol: chloroform (25:75), Whatman 3MM paper and ethanol:chloroform (25:75), and the more expensive ITLC-SG and 1-propanol: chloroform (10:90) were suitable systems for the direct determination of radiochemical purity of [{sup 99m}Tc(MIBI){sub 6}]{sup +} since impurities such as {sup 99m}Tc-reduced-hydrolyzed (RH), {sup 99m}TcO4{sup -} and [{sup 99m}Tc(cysteine){sub 2}]{sup -} complex were completely separated from the radiopharmaceutical, which moved toward the front of chromatographic systems while impurities were retained at the origin. The time required for analysis was 4 to 15 min, which is appropriate for nuclear medicine routines. (author)

  10. Study on technetium-99m labeling of graphene oxide nanosheets through click chemistry-99mTc labeling of graphene oxide nanosheets

    Institute of Scientific and Technical Information of China (English)

    江大卫; 彭程; 孙艳红; 贾丽娜; 李剑波; 张岚

    2015-01-01

    Graphene oxide (GO) nanosheets possess several advantages, such as a large surface, outstanding bio-compatibility, and straightforward chemical modification capability. They also have great potential as a drug-carrier. In this article, we radiolabeled GO nanosheets with 99mTc, which satisfies the potential needs of micro-SPECT imaging probes in pre-clinical and clinical research. GO nanosheets were synthesized through the modified Hummers’ method, then GO nanosheets with azide group covalently functionalized in two steps were conjugated to DOTA (1,4,7,10-tetraazacyclododecane-N,N0,N00,N000-tetraacetic acid) and functionalized with an alkynyl group by means of click chemistry. Then through the addition and reduction of technetium-99m, the 99mTc-DOTA-GO were attained. DOTA-conjugated GOs with lateral dimensions of 500–600 nm were synthe-sized. Both atomic force microscopy (AFM) and FT-IR were performed to characterize the GO-DOTA. Labeling efficiency of GO-DOTA with 99mTc was>90%and radiochemical purities were>96%with purification. We successfully synthesized graphene oxide derivatives, DOTA-conjugated GOs, via Click Chemistry, and it was labeled with 99mTc for SPECT imaging. High radiolabeling efficiency makes GO nanosheets suitable platforms for future molecular imaging research.

  11. Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI6]+

    Directory of Open Access Journals (Sweden)

    D.P. Faria

    2015-01-01

    Full Text Available Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [99mTc(MIBI6]+ as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol:chloroform (25:75, Whatman 3MM paper and ethanol:chloroform (25:75, and the more expensive ITLC-SG and 1-propanol:chloroform (10:90 were suitable systems for the direct determination of radiochemical purity of [99mTc(MIBI6]+ since impurities such as 99mTc-reduced-hydrolyzed (RH, 99mTcO4 - and [99mTc(cysteine2]- complex were completely separated from the radiopharmaceutical, which moved toward the front of chromatographic systems while impurities were retained at the origin. The time required for analysis was 4 to 15 min, which is appropriate for nuclear medicine routines.

  12. Genotoxic effect of radio marked lymphocytes using Tc-99m complexes; Efecto genotoxico del radiomarcado de linfocitos empleando complejos de Tc-99m

    Energy Technology Data Exchange (ETDEWEB)

    Pedraza L, M.; Ferro F, G.; Mendiola C, M.T.; Morales R, P. [Instituto nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1997-07-01

    The genotoxic effect of radio marked lymphocytes was evaluated using {sup 99m}-Tc-HMPAO and {sup 99m}-Tc- gentisic acid complexes. With the results of this work it is pretended to contribute to the knowledge of genetic and structural damages that provokes the radiation in the marked lymphocytes. The d, 1-HMPAO was synthesized in laboratory with a yielding of 30 %. The radiochemical purity of the complexes was greater than 85%. Mouse lymphocytes obtained of sanguineous volumes 2 ml were used. The radio marked efficiency of cells was 19.6 {+-} 6.4% and 25.6 {+-} 5.8% for {sup 99m}Tc-HMPAO and {sup 99m} Tc gentisic acid respectively. The genotoxic effect was evaluated using the technique of Unicellular Electrophoresis in Micro gel (Comet assay). The results showed that both {sup 99m} Tc complexes produce genotoxicity due to their capacity to penetrate cells, therefore the Auger and M internal conversion electrons place all their energy obtaining doses of Gray order. (Author)

  13. Experimental study of {sup 99m}Tc-aluminum oxide use for sentinel lymph nodes detection

    Energy Technology Data Exchange (ETDEWEB)

    Chernov, V. I., E-mail: Chernov@oncology.tomsk.ru; Sinilkin, I. G.; Zelchan, R. V.; Medvedeva, A. A. [Tomsk Cancer Research Institute, Kooperativny Street 5, Tomsk, 634050 (Russian Federation); Tomsk Polytechnic University, Lenin Avenue 30, Tomsk, 634050 (Russian Federation); Lyapunov, A. Yu., E-mail: Lyapunov1720.90@mail.ru [Tomsk Cancer Research Institute, Kooperativny Street 5, Tomsk, 634050 (Russian Federation); Bragina, O. D.; Varlamova, N. V.; Skuridin, V. S. [Tomsk Polytechnic University, Lenin Avenue 30, Tomsk, 634050 (Russian Federation)

    2016-08-02

    The purpose of the study was a comparative research in the possibility of using the radiopharmaceuticals {sup 99m}Tc-Al{sub 2}O{sub 3} and {sup 99m}Tc-Nanocis for visualizing sentinel lymph nodes. The measurement of the sizes of {sup 99m}Tc-Al{sub 2}O{sub 3} and {sup 99m}Tc-Nanocis colloidal particles was performed in seven series of radiopharmaceuticals. The pharmacokinetics of {sup 99m}Tc-Al{sub 2}O{sub 3} and {sup 99m}Tc-Nanocis was researched on 50 white male rats. The possibility of the use of {sup 99m}Tc-Al{sub 2}O{sub 3} and {sup 99m}Tc-Nanocis for lymphoscintigraphy was studied in the experiments on 12 white male rats. The average dynamic diameter of the sol particle was 52–77 nm for {sup 99m}Tc-Al{sub 2}O{sub 3} and 16.7–24.5 nm for {sup 99m}Tc-Nanocis. Radiopharmaceuticals accumulated in the inguinal lymph node in 1 hour after administration; the average uptake of {sup 99}mTc-Al{sub 2}O{sub 3} was 8.6% in it, and the accumulation of {sup 99m}Tc-Nanocis was significantly lower—1.8% (p < 0.05). In all study points the average uptake of {sup 99m}Tc-Al{sub 2}O{sub 3} in the lymph node was significantly higher than {sup 99m}Tc-Nanocis accumulation. The results of dynamic scintigraphic studies in rats showed that {sup 99m}Tc-Al{sub 2}O{sub 3} and {sup 99m}Tc-Nanocis actively accumulated into the lymphatic system. By using {sup 99m}Tc-Al{sub 2}O{sub 3} inguinal lymph node was determined in 5 minutes after injection and clearly visualized in all the animals in the 15th minute, when the accumulation became more than 1% of the administered dose. Further observation indicated that the {sup 99m}Tc-Al{sub 2}O{sub 3} accumulation reached a plateau in a lymph node (average 10.5%) during 2-hour study and then its accumulation remained practically at the same level, slightly increasing to 12% in 24 hours. In case of {sup 99m}Tc-Nanocis inguinal lymph node was visualized in all animals for 15 min when it was accumulated on the average 1.03% of the administered dose

  14. Labeling of thymidine analog with an organometallic complex of technetium-99m for diagnostic of cancer: radiochemical and biological evaluation; Marcacao de analogo da timidina com complexo organometalico de tecnecio-99m para diagnostico de cancer: avaliacao radioquimica e biologica

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Rodrigo Luis Silva Ribeiro

    2007-07-01

    Thymidine analogs have been labeled with different radioisotopes due to their potential in monitoring the uncontrollable cell proliferation. Considering that the radioisotopes technetium-99m still keep a privileged position as a marker due to its chemical and nuclear properties, this dissertation was constituted by the developed of a new technique of labeling of thymidine analog with {sup 99m}Tc, by means of the organometallic complex. The aims of this research were: synthesis of the organometallic complex technetium-99m-carbonyl, thymidine labeling with this precursor, evaluation of stability, and radiochemical e biological evaluation with healthy and tumor-bearing animals. The preparation of the organometallic precursor, using the CO gas, was easily achieved, as well as the labeling of thymidine with this precursor, resulting itself a radiochemical pureness of {>=} 97% and {>=} 94%, respectively. Chromatography systems with good levels of trustworthiness were used, ensuring the qualification and quantification of the radiochemical samples. The result of in vitro testing of lipophilicity disclosed that the radiolabeled complex is hydrophilic, with a partition coefficient (log P) of -1.48. The precursor complex and the radiolabeled have good radiochemical stability up to 6 h in room temperature. The cysteine and histidine challenge indicated losses between 8 and 1 1 % for concentrations until 300 mM. The biodistribution assay in healthy mice revealed rapid blood clearance and low uptake by general organs with renal and hepatobiliary excretion. The tumor concentration was low with values of 0.28 and 0.18 %ID/g for lung and breast cancer, respectively. The results imply more studies in other tumor models or the modification of the structure of the organic molecule that act like ligand. (author)

  15. Preparation and Biodistribution of 99mTc-Pamidronate as Bone Imaging Agent

    Directory of Open Access Journals (Sweden)

    LUO Hong-yi

    2016-02-01

    Full Text Available Labeling of Pamidronate (PAM with 99mTc was studied by a direct labeling method in the presence of SnCl2•2H2O as reducing agent. The influences of the concentration of SnCl2•2H2O, PAM concentration and pH value, reaction time on labeling yield were investigated. The optimum labeling was determined. The results showed that the radiochemistry purity of 99mTc-PAM was more than 95%. Biodistribution studies in normal mice and rats showed very high uptake of 99mTc-PAM and long retain in bone. 99mTc-PAM was washed out from the blood very quickly. In addition, considerable uptake in the kidneys indicated this complex was excreted mainly by renal pathway. On the other hand, the radioactivity in liver, lung and heart was negligible. It could be known from SPECT images that 99mTc-PAM would be an excellent bone-imaging agent. Bone uptake of 99mTc-PAM was higher than that of 99mTc-MDP in mice. This study suggested that 99mTc-PAM was a promising bone imaging agent and further study was worthwhile.

  16. Placental localization in abdominal pregnancy using technetium-99m-labeled red blood cells

    Energy Technology Data Exchange (ETDEWEB)

    Martin, B.; Payan, J.M.; Jones, J.S.; Buse, M.G. (Medical Univ. of South Carolina, Charleston (USA))

    1990-06-01

    In a patient with third trimester abdominal pregnancy with fetal demise, technetium-99m-labeled erythrocytes ({sup 99m}Tc-RBCs) localized the placenta preoperatively, after nonvisualization by ultrasonography and arteriography. Extrauterine placental localization by blood-pool imaging may be useful when ultrasound fails.

  17. Incidental detection of a bleeding gastrointestinal stromal tumor on Tc-99m red blood cell scintigraphy.

    Science.gov (United States)

    Santhosh, Sampath; Bhattacharya, Anish; Gupta, Vikas; Singh, Rajinder; Radotra, Bishan Dass; Mittal, Bhagwant Rai

    2012-10-01

    The role of 99m-technetium labeled red blood cell (RBC) scintigraphy in acute gastro-intestinal bleed is well-established. The authors report a case of a bleeding gastrointestinal stromal tumor (GIST) incidentally discovered on Tc-99m RBC scintigraphy.

  18. In Vitro Evaluation of Tc-99m Radiopharmaceuticals for Gastric Emptying Studies

    Directory of Open Access Journals (Sweden)

    Türkan Ertay

    2014-02-01

    Full Text Available Objective: Gastrointestinal motility and functional motility disorders causing either delayed or accelerated gastric emptying (GE may result in similar symptoms including nausea, vomiting, early satiety, fullness, bloating, and abdominal discomfort or pain. Hence, it is important to evaluate patients for both rapid and delayed GE in the same test. The gold standard technique to measure GE is scintigraphy by radiolabeled test meals. The aim of this study was to test alternative Tc-99m agents to label eggs as the solid meal and compare to Tc-99m sulfur colloid (SC for gastric emptying studies. Methods: In search of alternative agents for gastric emptying studies, we mixed and fried eggs with four different particulate compounds (Tc-99m labeled SC, tin colloid, nanocolloid and MAA, as well as with free pertechnetate and Tc-99m DTPA. We then measured the stability of these compounds in simulated gastric juice. Results: Our experiments demonstrated that in addition to Tc-99m sulfur colloid;Tc-99m MAA, Tc-99m nanocolloid and Tc-99m tin colloid also appear to make stable complexes with eggs in acidic environment. Conclusion: Therefore, these agents may be used for gastric emptying studies which could be more practical in routine conditions.

  19. Labeling of thymine with {sup 99m} technetium: a suggestion of a chemical model

    Energy Technology Data Exchange (ETDEWEB)

    Gutfilen, Bianca; Silva, Claudia Ribeiro da; Bernardo Filho, Mario [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Biologia. Dept. de Biofisica e Biometria; Ribeiro, Barbara Luzia Almeida [Instituto Nacional do Cancer, Rio de Janeiro, RJ (Brazil). Centro de Pesquisa Basica; Mattos, Maura Ferreira [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Quimica

    1996-03-01

    Successful targeting of diagnose but also to stage cancer. It has been shown that certain tumor cells are permeable to low level of exogenous adenosine-diphosphate and adenosine-triphosphate nucleotides, that are incorporated into intracellular pools. We present the labeling of a nucleotide precursor, a base, thymine technetium-99m ({sup 99m} Tc). (author)

  20. Sensitivity of [Tc-99m]methoxyisobutylisonitrile scan in patients with metastatic differentiated thyroid cancer

    NARCIS (Netherlands)

    Ronga, G.; Ventroni, G.; Montesano, T.; Filesi, M.; Ciancamerla, M.; Di Nicola, A. D.; Travascio, L.; Viestri, A. R.; Signore, A.

    2007-01-01

    Aim. The aim of the present study was to evaluate the diagnostic sensitivity of [Tc-99m]methoxyisobutylisonitrile ([Tc-99m]MIBI) in a large series of patients with metastatic differentiated thyroid carcinoma (DTC), as compared with I-131-whole body scan (WBS) and other diagnostic imaging techniques.

  1. Simulation of the direct production of 99mTc at a small cyclotron

    Science.gov (United States)

    Eslami, M.; Kakavand, T.

    2014-06-01

    Usually 99mTc is produced indirectly through generator 99Mo/99mTc. In the present study, the direct production of this radioisotope by charged particle irradiation was investigated using Monte Carlo method. After scouting of the reactions that produce 99mTc, excitation functions of these reactions were predicted by optical model components in the TALYS-1.6 code. Suitable energy range of projectile for this production was selected by spotting of maximum cross section and minimum impurity due to other emission channels. Then target geometry was designed based on stopping power calculation by the SRIM code. Thick target yield of 100Mo(p,2n)99mTc, 98Mo(p,γ)99mTc and natMo(p,x)99mTc reactions was predicted by the result of excitation function and stopping power calculations. Finally, 100Mo(p,2n)99mTc reaction was selected as a primary reaction for the direct production of 99mTc and its process was simulated by employing the MCNPX code to calculate the energy distribution of proton in the 100Mo target body and estimation of residual nuclei during irradiation. Good agreement was obtained between the experimental, the theoretical, and the simulation-based (analytical and directly) production yields. This study demonstrated that Monte Carlo provides a method for the design and optimization of targets for the radionuclide production purposes.

  2. Clinical evaluation of hepatic scintigraphy using sup 99m Tc-GSA

    Energy Technology Data Exchange (ETDEWEB)

    Ishii, Katsumi; Nishiyama, Shogo; Nishimaki, Hiroshi; Tadokoro, Katsumi; Ikeda, Toshiaki; Matsubayashi, Takashi; Ishii, Kodo (Kitasato Univ., Sagamihara, Kanagawa (Japan). School of Medicine)

    1992-06-01

    Functional hepatic imaging was performed using {sup 99m}Tc-DTPA-galactosyl human serum albumin ({sup 99m}Tc-GSA), a radiolabeled ligand that reacts specifically to the asialoglycoprotein receptor that resides at the plasma membrane of hepatocytes, in 21 patients: five with chronic active hepatitis (CAH), 14 with compensative liver cirrhosis (LC), one with chronic inactive hepatitis and one with acute hepatitis. The former two diseases were mainly investigated. Serial liver images were acquired at the rates of 10 sec/frame for 0-5 min and 2 min/frame for 6-30 min after the injection of {sup 99m}Tc-GSA, and the images were compared with {sup 99m}Tc-phytase images in 2 patients with CAH and 11 with LC, and those with portal scintigrams using {sup 123}I-iodoamphetamine (IMP) in 3 patients with LC. The images using {sup 99m}Tc-GSA were in better agreement with hepatic function than those using {sup 99m}Tc-phytase, and with the findings of portal scintigraphy using {sup 123}I-IMP. LHL15 (liver/liver and heart radioactivities at 15 min after injection of {sup 99m}Tc-GSA) correlated with the hepaplastin test (r=0.978 in CAH, and r=0.544 in LC), indicators of hepatic reserve. These results suggest that liver scintigraphy using {sup 99m}Tc-GSA might be a useful method for evaluating liver function. (author).

  3. Tc-99m-HMPAO brain scintigraphy. A comparative study with I-123-IMP

    Energy Technology Data Exchange (ETDEWEB)

    Nishimoto, Hitoshi; Yamamoto, Yoichi; Yoshida, Shoji; Akagi, Naoki; Ohara, Shuichi; Kamiike, Osamu; Maeda, Tomoho

    1989-04-01

    Sixteen single photon emission computed tomography (SPECT) studies with Tc-99m hexamethyl propylene amine oxime (Tc-99m HMPAO) were performed in patients with cerebral infarction (n=10), transient ischemic attack (n=2), or brain tumor (n=3). X-ray computed tomography (CT) scans and SPECT scans with N-isopropyl-p-(I-123)iodoamphetamine (I-123 IMP) were also obtained for comparison with findings of SPECT with Tc-99m HMPAO. Crossed cerebellar diaschisis (CCD) was detected in five patients (33%) on both early and delayed SPECT scans with Tc-99m HMPAO. In 8 patients, early scans with Tc-99m HMPAO tended to depict lesions more extensively when compared with CT. Early scanning with I-123 IMP was superior to that with Tc-99m HMPAO in detecting lesions. In detecting CCD, both methods were comparable to each other. In visualizing the basal ganglion, early scans with Tc-99m HMPAO were superior to scans with I-123 IMP. Tc-99m HMPAO has the advantages of short half-life period, small amounts of exposure doses, simple labeling, and prompt usage in emergency cases, such as cerebral infarction and cerebrovascular disorder. (Namekawa, K).

  4. 99mTc-YIGSR as a Receptor Tracer in Imaging the Ehrlich Ascites Tumor-bearing Mice as Compared with 99mTc-MIBI

    Institute of Scientific and Technical Information of China (English)

    HU Jia; QIN Guangming; ZHANG Yongxue; AN Rui; LAN Xiaoli

    2007-01-01

    The validity of 99mTc-YIGSR, a novel receptor radio-tracer, in imaging the Ehrlich ascites tumor was evaluated. YIGSR, a pentapeptide of laminin, was labeled with 99mTc by using a bifunctional chelator S-Acetly-NH3-MAG3. The MIBI was labeled with 99mTc by following the kit instruction. The mice of tumor group were intravenously injected 1-2 mCi of 99mTc-YIGSR or 99mTc-MIBI via caudal vein, immobilized and imaged under a Gamma camera. The same procedure was performed in mice of blockade group, in which the unlabeled YIGSR was previously injected to block the receptor-recognition sites, and inflammation group serving as control. The reverse-phase Sep-Pak C18 chromatogram was found to have an essentially complete conjugation between YIGSR and S-Acetly-NH3-MAG3. The conjugated YIGSR could be radio-labeled successfully with 99mTc at room temperature and neutral pH, with a radio-labeling yield of 62%. Without the chelator S-Acetly-NH3-MAG3, the YIGSR was labeled with 99mTc at an efficiency of 4%. The imagological study revealed obvious tumor accumulation of 99mTc-YIGSR 15 min after the injection, and the uptake peaked after 3 h with a tumor-to-muscle ratio (T/M) of 11.36. The radio-tracer was slowly cleared up and resulted in a T/M of 3.01 at the 8th h after the injection. As for blocked group, the tumor uptake of radiotracer was significantly lower, with the highest T/M being 4.61 after 3 h and 0.89 after 8 h. The T/M was 3.72 at the 3rd h and 1.29 at the 8th h after the 99mTc-YIGSR injection in the inflammatory group. The T/M was significantly higher in tumor group than in inflammatory group or control group (P<0.001). In the 99mTc-MIBI group, the T/M was 1.40 at the 3rd h and 0.55 at the 8th h after the injection, which showed a significant difference as compared with 99mTc-YIGSR (P<0.001).It is concluded that YIGSR can be successfully radiolabelled by using S-Acetly-NH3-MAG3.99mTc-YIGSR has many advantages in tumor imaging, such as quick and clear visualization

  5. Tc-99m sestamibi parathyroid gland scintigraphy: added value of Tc-99m pertechnetate thyroid imaging for increasing interpretation confidence and avoiding additional testing.

    Science.gov (United States)

    Powell, Daniel K; Nwoke, Franklin; Goldfarb, Richard C; Ongseng, Fukiat

    2013-01-01

    To evaluate the benefit of adding a pertechnetate parathyroid scan (dual-isotope imaging) in the interpretation of sestamibi dual-phase parathyroid scintigraphy. One hundred and sixteen dual Tc-99m sestamibi (MIBI) and Tc-99m pertechnetate subtraction parathyroid studies, performed between January 2000 and February 2006, were retrospectively reviewed. Dual-phase technetium sestamibi examinations were initially interpreted, with blinding to the technetium pertechnetate findings. Subsequently, technetium pertechnetate scan findings were added, and changes in interpretation were recorded. By adding Tc-99m pertechnetate imaging, the interpretation of 17 scans (17/116=14.6%) was substantially altered. This included 5 scans (4%) that changed from negative to positive and 9 scans (8%) that changed from equivocal to positive, excluding ectopic tissue and directing minimally invasive surgery, without the need for further imaging, such as ultrasound, in 12% of cases. One examination changed from positive to negative. In addition, 2 scans changed from equivocal to negative, necessitating further preoperative imaging for the evaluation of additional pathology such as thyroid nodules and lymph nodes and the consideration of hyperplasia. Among the remaining 99 patients, Tc-99m pertechnetate scans may also have contributed to the diagnosis in the 66 positive Tc-99m MIBI scans by increasing confidence in the interpretation and obviating additional imaging. Ten cases remained equivocal. By adding Tc-99m pertechnetate imaging, scan interpretation was changed in 14.6% of cases, and interpretation confidence was enhanced in all but 10 remaining equivocal cases. The addition of a dual-isotope subtraction also eliminated the need for additional testing, such as ultrasound, in 12% of our cases. Increased confidence in interpretation that comes with dual-isotope subtraction may come at the cost of slight lengthening of imaging time but likely simplifies preoperative localization and

  6. Technetium-99m labelled human immunoglobulin scintigraphy predicts rheumatoid arthritis in patients with arthralgia

    Energy Technology Data Exchange (ETDEWEB)

    Bois, M.H.W. de; Arndt, J.W.; Speyer, I.; Pauwels, E.K.J.; Breedveld, F.C.

    1996-05-01

    The ability of {sup 99m}Tc-IgG scintigraphy to predict the development of rheumatoid arthritis (RA) in 47 patients with arthralgia was investigated. {sup 99m}Tc-IgG scintigraphy and the serum test for rheumatoid factor (RF), measured at the beginning of a year long study, were compared for their ability to predict RA. During the study 8 patients developed RA. The specificity and positive predictive values of RF in predicting RA were 79% and 50% respectively, and for {sup 99m}Tc-IgG scintigraphy 97% and 88%. The sensitivity and negative predictive values of RF were 100% and of {sup 99m}Tc-IgG-scintigraphy 88% and 97%, respectively. In conclusion, {sup 99m}Tc-IgG scintigraphy has additional value to RF with respect to the prediction of the development of RA in patients with arthralgia. 27 refs.

  7. Incidental detection of gastrointestinal stromal tumor by Tc-99m MDP bone scan.

    Science.gov (United States)

    Shepherd, Timothy M; Idakoji, Ibrahim A; Pampaloni, Miguel H

    2012-02-01

    This case demonstrates extraosseous 99m-technetium methylene diphosphonate (Tc-99m MDP) accumulation from a gastrointestinal stromal tumor. A 75-year-old woman underwent a temporal bone CT for conductive hearing loss that showed sclerosis in the right occipital condyle. Follow-up Tc-99m MDP bone scan for osseous metastases instead showed a mass-like extraosseous accumulation of Tc-99m MDP in the anterior left upper quadrant. Differential diagnoses included gastric cancer, lymphoma, metastatic melanoma, systemic hypercalcemia, or heterotopic mesenteric ossification. Contrast CT showed a well-circumscribed mass arising from the stomach, and subsequent pathology confirmed gastrointestinal stromal tumor. These tumors rarely can contain osteoclast-like giant cells and should be considered for extraosseous Tc-99m MDP accumulation.

  8. Synthesis and biodistribution of {sup 99m}Tc-Vancomycin in a model of bacterial infection

    Energy Technology Data Exchange (ETDEWEB)

    Roohi, S.; Mushtaq, A. [Isotope Production Div., Pakistan Inst. of Nuclear Science and Technology, Islamabad (Pakistan); Malik, S.A. [Dept. of Biological Sciences, Quaid-e-Azam Univ., Islamabad (Pakistan)

    2005-07-01

    Vancomycin Hydrochloride is an antibiotic produced by the growth of certain strains of Streptomyces orientalis. As vancomycin hydrochloride is poorly absorbed after oral administration; it is given intravenously for therapy of systemic infections. Vancomycin was labeled with technetium-99m pertechnetate using SnCl{sub 2} . 2H{sub 2}O as reducing agent. The labeling efficiency depends on ligand/reductant ratio, pH, and volume of reaction mixture. Radiochemical purity and stability of {sup 99m}Tc-Vancomycin was determined by thin layer chromatography. Biodistribution studies of {sup 99m}Tc-Vancomycin were performed in a model of bacterial infection in Sprague-Dawley rats. A significantly higher accumulation of {sup 99m}Tc-Vancomycin was seen at sites of S. aureus infected animals. Whereas uptake of {sup 99m}Tc-Vancomycin in turpentine inflamed rats were quite low. (orig.)

  9. Preparation and evaluation of (99m)Tc-DMSA lyophilized kit for renal imaging.

    Science.gov (United States)

    Jan, Syed Umer; Abbass, Hafiz Ghulam

    2013-05-01

    Dimercaptosuccinic acid (DMSA) has been evaluated and used with technetium 99m ((99m)Tc) in imaging of kidneys. DMSA lyophilized kits were prepared and radiolabelled with (99m)Tc. Paper and thin-layer chromatography have been employed using various eluent systems for the radiochemical analysis, percentage labeling and binding capacity of (99m)Tc-DMSA. Female albino rabbits were used for this study. Biological data obtained after intravenous injection of radiolabelled DMSA to female albino rabbits revealed 32.42% uptake and long retention time in the kidneys. On the basis of animal biodistribution data, it is suggested that DMSA when labeled with (99m)Tc is useful complex for renal imaging and can be successfully applied as a diagnostic tool in nuclear medicine. Clinical biodistribution and radiation dosimetry studies are planned in future.

  10. Accumulation velocity of /sup 99m/Tc-HMDP in hip joint diseases

    Energy Technology Data Exchange (ETDEWEB)

    Torizumi, Kazutami; Aibata, Hirofumi; Kiji, Shigeyuki; Ueyoshi, Akitaka; Kiura, Yoshifumi; Okamoto, Yukiharu; Tuda, Tadaaki; Ota, Kiichiro

    1988-07-01

    The accumulation rate and velocity of /sup 99m/Tc-hydroxymethylene diphosphonate (/sup 99m/Tc-HMDP) in hip joints were determined within four minutes after a bolus of injection of /sup 99m/Tc-HMDP in the patients with hip joint disorders. There exist a significant relationship between accumulation velocity of /sup 99m/Tc-HMDP in the affected joint and serum osteocalcin in the patients with eight cases of degenerative osteoarthropathy and six cases of femoral necrosis. This result indicates that the accumulation velocithy of /sup 99m/Tc-HMDP in the affected joint is likely to become a marker of bone turnover in hip joint disorder.

  11. Synthesis and preliminary biological evaluation of a technetium-99m labeled thymidine analog

    Institute of Scientific and Technical Information of China (English)

    Chun Xiong Lu; Zheng Wu Wang; Quan Fu Jiang; Jie Tang; Cheng Tan; Jian Kang Zhang

    2011-01-01

    The synthesis and labeling of 99mTc-N3-{N'-[2-sulfanyl-ethylamino)acetyl]-2-aminoethyl-sulfanyl-l-hexanamide}thymidine (99mTc-NHT) were studied. In the presence of sodium glucoheptonate (GH) and ethylene diamine tetraacetic acid (EDTA), 99mTc-NHT was obtained by using bisaminoethanethiol (N2S2) as a bifunctional coupling agent. The radiochemical purity of the 99mTc-NHT was over 95%. Biodistribution of 99mTc-NHT was performed in hepatoma HepA tumor-bearing mice. At 2 h p.i., the ratios of tumor-to-muscle, tumor-to-bone and tumor-to-blood were 4.41 ± 0.32, 2.45 ± 0.24 and 1.51 ±0.18, respectively.

  12. Effect of technetium-99m on iodine-131 thyroid uptake measurements

    Energy Technology Data Exchange (ETDEWEB)

    Wasserman, H.J.; Klopper, J.F.; Erlank, P.

    1985-06-01

    Following administration of 2.5-5.0 mCi(/sup 99m/Tc)pertechnetate, several hundred microcuries may be present in the necks of hyperthyroid patients after 6 hr. Coincidence summing of Tc-99m photons may disturb I-131 uptake measurements in such patients if an oral diagnostic dose of I-131 is administered immediately after completion of a (/sup 99m/Tc)pertechnetate scintigram. Using a lower discriminator level of 300 keV, false increases of 10%-50% may occur at 6 hr. The 24-hr uptake is also affected if an I-131 predose measurement is performed after administration of the Tc-99m dose. The authors have shown that these errors may be prevented by using a 1-mm lead filter in front of the scintillation detector. The attenuation of Tc-99m photons by the filter effectively eliminates summation pulses while it reduces the I-131 count rate by approximately 28%.

  13. Biokinetic and dosimetry of {sup 99m}Tc-14F7-monoclonal antibody in patients with breast cancer

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, Joaquin; Calderon, Carlos; Pimentel, Gilmara; Oliva, Juan P., E-mail: jgg@infomed.sld.cu [Departmento de Medicina Nuclear, Instituto de Oncologia y Radiobiologia, Cidad Habana (Cuba); Casaco, Angel [Division de Ensayos Clinicos, Centro de Inmunologia Molecular, Reparto Siboney, Ciudad Habana (Cuba)

    2008-07-01

    Full text: The 14F7 murine MAb is an IgG1 that binds specifically to GM3(Neu-Gc) ganglioside strongly recognizing the antigen displayed in human breast and melanoma tumors. A preliminary report showed in vivo evidences of presence of GM3(Neu-Gc) ganglioside in human breast primary tumors using 14F7 MAb labelled with {sup 99m}Tc. In this work the pharmacokinetics and biodistribution of {sup 99m}Tc-14F7 MAb in patient with breast cancer were studied. The absorbed dose in normal organ and tumors was also calculated. The patients were selected from a Phase I/II clinical trial which included 14 female patients with cytological diagnose of breast carcinoma in stage II without previous onco-specific treatment. Three groups were conformed: Group I (0.3mg, n=5), Group II (1mg, n=5) and Group III (3mg, n=4). The activity ranged in 1.11 - 1.48GBq (30- 40mCi) was intravenously administered during 1-2min. The {sup 99m}Tc-14F7 plasma clearance was determined by blood sampling at 5, 10, 20, 30min and 1, 2, 4, 8 and 24h after injection. All urine excreted by patients was collected during 24h after injection at intervals of 0-2h, 2-8h and 8-24h. Prior to injection, a transmission scan of head, thorax, abdomen and pelvis was obtained using a {sup 57}Co flood source. Anterior and posterior whole body images were acquired at 30min, 2h, 4h, 8h and 24h after injection using a Gamma Camera (SophyCamera DS7) with pinhole collimator. Static anterior and posterior images were obtained from head, thorax, abdomen, pelvis and lateral images of each breast in pendular position at 2h, 4h, 8h and 24h. Internal absorbed dose calculation was performed according to MIRD formalism using the S values for adult non-pregnant female phantom downloaded from the RADAR website. Self-absorbed tumor dose were calculated assuming those as spheres with uniform distribution of activity. The plasma clearance of radiotracer in all groups shows a monoexponential decay behaviour with biological elimination half time

  14. Contact thermography, 99mTc-plasmin scintimetry and 99mTc-plasmin scintigraphy as screening methods for deep venous thrombosis following major hip surgery

    DEFF Research Database (Denmark)

    Christensen, S W; Wille-Jørgensen, P; Kjaer, L

    1987-01-01

    Fifty-six patients scheduled for total hip alloplasty were screened for deep venous thrombosis by means of 99mTc-plasmin scintimetry, 99mTc-plasmin scintigraphy and contact thermography. Investigations were performed on the seventh postoperative day, and a total of 112 legs were examined. Bilateral....... The nosographic sensitivity/specificity was 33%/75% for scintimetry, 50%/91% for scintigraphy and 33%/87% for contact thermography, respectively. It is concluded that all three tests are of no value as screening methods for deep venous thrombosis following major elective hip surgery....

  15. [Diagnostic possibilities of static gamma-scintigraphy using 99mTc pyrophosphate and Tc 99m phosphate in children with glomerulo nephritis].

    Science.gov (United States)

    Mil'ko, V I; Bagdasarova, I V; Moskalenko, N I; Ivanov, D D

    1989-01-01

    Children with nephrotic glomerulonephritis were examined for incorporation of 99mTc-pyrophosphate and 99mTc-phosphone. The relationship was discovered between the accumulation of the radiopharmaceutical drug and the sensitivity to glucocorticoids used in the pathogenetic treatment. Accumulation of the drug in the course of the treatment documents the presence of complete, partial remission or the disease activity. It is recommended that static gammascintigraphy should be carried out for early assessment of the efficacy of the treatment and its correction.

  16. The preparation of {sup 99m}Tc from {sup 99}Mo-{sup 99m}Tc using Poly-Zirconium Compound (PZC)

    Energy Technology Data Exchange (ETDEWEB)

    Laohawilai, S. [Isotope Production Div., Office of Atomic Energy for Peace (Thailand)

    1998-10-01

    The process of for providing the {sup 99m}Tc-gel generator followed the protocol that received from Department of Radioisotopes, Tokai Research Establishment, JAERI. Sodium molybdate (molybdenum-99), pH 7 with concentration 20 mg/ml and activity nearly 1 mCi/ml was added into the adsorbent (PZC). The total activity of {sup 99}Mo was about 10 mCi and the heating time was varied from 3-18 hours at 70degC. The properties of the gel were studied for elution profile and elution efficiency. The molybdenum breakthrough in sodium pertechnetate (technetium-99m) was also performed. (author)

  17. 99mTc-pyrophosphate scintigraphy in the diagnosis of acute myocardial infarction. Myokardscintigrafi med 99mTc-pyrofosfat ved akutt hjerteinfarkt

    Energy Technology Data Exchange (ETDEWEB)

    Hole, T.; Rokkones, T. (Central Hospital in Moere og Romsdal, Aalesund (Norway))

    1990-02-01

    50 consecutive patients, hospitalized because of suspected myocardial infarction, were examined by 99mTc-pyrophosphate scintigraphy. The scans were interpreted blind by one of the authors. There were positive results in 17 of 21 patients with myocardial infarction (sensivity 0.81). However, scintigraphy was also positive in 13 patients with no evidence of myocardial infarction as asessed by ECG and enzyme criteria (specificity 0.55). It is concluded that 99mTc-pyrophosphate myocardial scintigraphy did not contribute valuable information to the routine diagnosis of myocardial infarction. 11 refs.; 3 tabs.

  18. Bowel activity caused by free Tc-99m pertechnetate mimicking urine leaks during Tc-99m MAG{sub 3} renal scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Lim, Seok Tae; Kim, Min Woo; Sohn, Myung Hee [Chonbuk National University Medical School, Chonju (Korea, Republic of)

    2003-04-01

    A 43-year-old woman with diabetic nephropathy underwent a Tc-99m MAG3 renal scintigraphy for the evaluation of renal function. Posterior images at 60 minutes demonstrated a migration of radiotracer activity beyond the lower pole of the left kidney, which might be incorrectly interpreted as urine leaks. However, the increased activities were moving along the bowel lumens over time. Another ring-like radioactivity was also seen in the suprasplenic region, and increased with time. These radioactivities were in the gastric fundus and gastrointestinal tract and caused by free Tc-99m pertechnetate.

  19. Technetium-99m hexamethylpropylene amine oxime lung scintigraphy findings in low-dose amiodarone therapy.

    Science.gov (United States)

    Kaya, G Capa; Ertay, T; Tuna, B; Bekis, R; Tasci, C; Sayit, E; Yilmaz, O; Kargi, A; Durak, H

    2006-01-01

    Amiodarone (AD)-induced pulmonary toxicity is one of the major complications of long-term AD therapy. Technetium-99m-labeled D: ,L: -hexamethylpropylene amine oxime (Tc-99m HMPAO) scintigraphy has been used to assess lung injury. We designed this study to clarify lung uptake changes of Tc-99m HMPAO using low doses of AD (5 mg/kg/day) during long-term therapy in a rabbit model. Group 1 consisted of 7 rabbits fed with AD by gavage for 6 months. To investigate the effect of ketamine on Tc-99m HMPAO uptake, 5 rabbits were included in Group 2 as a control group. Tc-99m HMPAO scintigraphy was performed in both Group 1 and Group 2 at baseline and after 2, 4, 6, 8, and 12 weeks of AD intake. After 16, 20, and 24 weeks of drug intake, Tc-99m HMPAO scintigraphy was repeated only in group 1. One-min anterior images were acquired 30 min after the injection of 37 MBq of Tc-99m HMPAO. For semiquantitative evaluation, the mean count values were obtained and lung/background and liver/background ratios were calculated. Histopathologic evaluation was performed. No increase in lung and liver uptake of Tc-99m HMPAO was found 2, 4, 6, 8, and 12 weeks after drug intake. There was no significant increase in L/B and H/B ratios of Tc-99m HMPAO in Group 1 compared with Group 2. Both scintigraphic studies and histopathologic examinations showed nonspecific changes. Longitudinal studies investigating Tc-99m HMPAO lung uptake may be planned in patients carrying risk factors for AD-induced lung toxicity.

  20. Anti-MUC1 aptamers: radiolabelling with {sup 99m}Tc and biodistribution in MCF-7 tumour-bearing mice

    Energy Technology Data Exchange (ETDEWEB)

    Da Pieve, Chiara [Department of Chemistry and Analytical Sciences, Open University, Walton Hall, MK7 6AA Milton Keynes (United Kingdom); Perkins, Alan C. [Academic Medical Physics, School of Clinical Sciences, University of Nottingham, NG7 2UH Nottingham (United Kingdom); Missailidis, Sotiris [Department of Chemistry and Analytical Sciences, Open University, Walton Hall, MK7 6AA Milton Keynes (United Kingdom)], E-mail: s.missailidis@open.ac.uk

    2009-08-15

    Introduction: Aptamers previously selected against the protein core (AptA) or the tumour glycosylated (AptB) MUC1 glycoprotein have been conjugated to MAG2 and labelled with {sup 99m}Tc, for the potential use as radiopharmaceuticals for diagnostic imaging of breast cancer. Methods: The conjugation was achieved in high yield using standard peptide coupling reactions between an amino modification on the aptamer and the activated carboxylic group on the ligands. The retention of the affinity of the MAG2 modified AptA for the MUC1 protein core was confirmed using a fluorescent intercalator displacement binding assay. The labelled aptamers were separated from free {sup 99m}Tc using ultrafiltration and monitored by high-performance liquid chromatography at all stages, to ensure that only radiolabelled aptamers were produced. The biodistribution properties of the two aptamer-radionuclide conjugates were analysed in MCF-7 tumour bearing mice and compared. Results: Efficient and convenient labelling of the two aptamers with {sup 99m}Tc was achieved as the last step of the synthesis (post-conjugation labelling). Both the aptamer-chelator conjugates had strong {sup 99m}Tc binding properties and the resulting complexes were stable in vivo, both in terms of nuclease degradation and leaking of the metal. The radiolabelled aptamers showed a high renal clearance and a high uptake in the intestine. Conclusions: AptA and AptB have been successfully conjugated in high yield to the ligand MAG2 and labelled with {sup 99m}Tc. The radiolabelled aptamers showed different tumour uptake and clearance, but will require further development prior to diagnostic use.

  1. The simultaneous use of 99m-Tc-HIDA scintigraphy and ultrasound in determination of gallbladder storage and emptying in the fasting state

    DEFF Research Database (Denmark)

    Qvist, Niels; Rafaelsen, Søren Rafael; Øster-Jørgensen, E.

    1991-01-01

    In eight healthy fasting young men simultaneous hepatobiliary scintigraphy and ultrasonography of the gallbladder was performed using intravenous infusion of 99m-Tc-HIA administered at a rate of 40 Mbq/h. Time-activity curves over the gallbladder and intestinal area were generated. Gallbladder vo...

  2. Tc-99m-Human Serum Albumin Transit Time as a Measure of Arm Breast Cancer-Related Lymphedema

    DEFF Research Database (Denmark)

    Toyserkani, Navid M; Hvidsten, Svend; Tabatabaeifar, Siavosh

    2017-01-01

    34-68 years, with unilateral arm lymphedema following breast cancer treatment underwent bilateral lymphoscintigraphy using intradermal injection in both hands of technetium-99m-labeled human serum albumin and sequential 5 min imaging for 5 hours. The mean transit time (MTT) in the arms was calculated...... based on time activity curves generated from injection site and arm regions. Visual lymphedema scoring was performed based on dermal backflow and lymph node presence. Excess arm volume was calculated from circumference measurements. RESULTS: The MTT (mean ± SD) was significantly longer in the lymphedema......, 18.6-68.7 minutes; P lymphedema from...

  3. Capillary permeability of 99mTc-DTPA in canine myocardium determined by intracoronary bolus injection and residue detection

    DEFF Research Database (Denmark)

    Svendsen, Jesper Hastrup; Efsen, F; Haunsø, S

    1989-01-01

    Capillary permeability of 99mtechnetium-diethylenetriaminepenta-acetic acid (99mTc-DTPA; MW 485.0) and 51chromium-ethylenediaminetetra-acetate (51Cr-EDTA; MW 340.2) was studied in an in vivo canine heart preparation by the single injection, residue detection (SIRD) method. In experiments on open...... chest dogs (group A) these indicators were administered separately as bolus injections into a cannulated diagonal branch of the left anterior descending coronary artery (LAD) and the curve of the response function was recorded by external activity registration. In further experiments on closed and open...

  4. Extraction of [99mTc]-d,l-HM-PAO across the blood-brain barrier

    DEFF Research Database (Denmark)

    Andersen, A R; Friberg, H; Knudsen, K B

    1988-01-01

    The initial extraction (E) across the blood-brain barrier (BBB) of [99mTc]-d,l-HM-PAO after intracarotid injection was measured in 14 Wistar rats and 6 patients using the double indicator, single injection method with Na-24 as the cotracer. In both series, cerebral blood flow (CBF) was measured...... using the initial slope of the xenon-133 washout curve after intracarotid bolus injection. In rats, bolus size (20 or 120 microliters), bolus type (saline or 10% albumin), or CBF were changed. First-pass extraction was dependent on CBF (p less than 0.001): With a small bolus of saline and at resting CBF...... the apparent extraction across brain capillaries. In patients using a bolus of 1 ml saline, E decreased linearly with increasing CBF (r = -0.81, p less than 0.001). For a CBF of 0.59 ml/g/min and an average apparent E of 0.72, an apparent PS product of 0.76 ml/g/min was calculated.(ABSTRACT TRUNCATED AT 250...

  5. Evaluation of dynamic renal function by nuclear medicine using Tc-99m DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Ishibashi, M.

    1987-01-01

    Dynamic renal function was nucleomedically evaluated using Tc-99m DTPA, and its value was assessed in various renal diseases. In addition to RI angiography, sequential image and renogram curve which were traditionally used as routine studies, Renal First Blood Flow (RFBF) by aortic blood flow exactly at the entrance of the renal artery, GFR (DTPA-GFR) by renal uptake and mean transit time (MTT) of the tracer by deconvolution analysis were measured. RFBF was useful to evaluate the stricture at the confluence of the renal artery and renal circulation, particularly cortical circulation. DTPA-GFR enabled the simple and rapid measurement of Total-GFR and Split-GFR. MTT was considered to be valuable in diagnosing obstructive nephropathy. In particular, RFBF was useful for assessment of renovascular hypertension. The measurement of the above-mentioned parameters made it possible to evaluate renal function objectively and quantitatively in addition to imaging and semiquantitative studies obtained by traditional routine examination. The major advantage of this procedure includes its possible performance along with routine renal function tests without any special preparation.

  6. Gallbladder ejection fraction using {sup 99m}Tc-DISIDA scan in diabetic autonomic neuropathy

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Seong Jang; Kim, In Ju; Kim, Yong Ki; An, Jun Hyup [Pusan National Univ. Hospital, Pusan (Korea, Republic of); Yoo, Seok Dong [Dongkuk Univ. College of Medicine, Seoul (Korea, Republic of)

    2000-02-01

    We performed this study to evaluate the changes of gallbladder ejection fraction (GBEF) in diabetic patients with or without autonomic neuropathy. This study included 37 diabetic patients (25 women, 12 men, mean age 51 years) and 24 normal controls (10 women, 14 men, mean age 38 years). After intravenous injection of 185 MBq of {sup 99m}T{sub c}-DISIDA, serial anterior abdominal images were acquired before and after fatty meal. Regions of interest were applied on gallbladder and right hepatic lobe on 60 and 90 minute images to calculate GBEF. GBEF was significantly reduced in diabetes with autonomic neuropathy (43{+-}12.3%) and without autonomic neuropathy (57.5{+-}13.2%) compared with normal controls (68{+-}11.6%, p<0.05). And also, GBEF was significantly reduced in diabetes with autonomic neuropathy compared with diabetes without autonomic neuropathy (p<0.05). Fasting blood glucose level, age, sex, hemoglobin A1c, body mass index, serum lipid level were not different in these two diabetic patient groups (p>0.05). When 50.2% of GBEF was used as the criteria for diabetic autonomic neuropathy, the sensitivity and specificity were 80%, 76.5%, respectively. The area under receiver operating characteristic curve was 0.846. GBEF of diabetic patients with autonomic neuropathy was significantly reduced than that of diabetic patients without autonomic neuropathy.

  7. Splenic sequestration of Tc-99m labeled heat treated red blood cells. [Feasibility of use for spleen imaging

    Energy Technology Data Exchange (ETDEWEB)

    Atkins, H L; Goldman, A G; Fairchild, R G; Oster, Z H; Som, P; Richards, P; Meinken, G E; Srivastava, S C

    1979-01-01

    The rate of blood clearance and spleen uptake as well as the total spleen uptake of heat damaged red blood cells labeled with Tc-99m was determined in eight patients, six of whom had chronic lymphatic leukemia, one had polycythemia vera and one had eosinophilia of unknown origin. Spleen uptake at 2 hrs was 72.0 +- 18.5%. Approximately 82.6% of the initial radioactivity was cleared from the blood by 2 hrs with a rapid T 1/2 component of 6.3 +- 4.7 min. The T 1/2 of splenic uptake was 8.3 +- 4.6 min with a plateauing of splenic radioactivity by 30 min. The preliminary results indicate that the method of preparation is reliable but the usefulness of the method for evaluating spleen function remains to be determined.

  8. Blood flow and tracer uptake in normal and abnormal canine bone: comparisons with Sr-85 microspheres, Kr-81m, and Tc-99m MDP

    Energy Technology Data Exchange (ETDEWEB)

    Lavender, J.P.; Khan, R.A.A.; Hughes, S.P.F.

    1979-05-01

    The bone scan, showing the 4-hr residue in bone of technetium diphosphonate, is a widely used diagnostic tool. The question arises whether it reflects changes in local blood flow to bone, or avidity, or both. Two experimental approaches have been used in trying to answer this question. The first was to determine immediate single-passage clearance and 4-hr residue in normal and abnormal bone. If flow is the determinant of residue these should be the same since, in this event, extraction will not change. An osteotomy was produced in the dog tibia and retrograde catheterization of the anterior tibial artery allowed measured amounts of technetium-methylene-diphosphonate (Tc-99m MDP), to be infused into the nutrient artery. Similar preparation in normal bone allowed comparison of both clearance and 4-hr residue. Results have shown increased 4-hr residue 10 days after the osteotomy (normal 4.9% ..-->.. 13.4%). The second approach involved use of a gamma camera to record profiles of blood flow in normal and abnormal bone: (a) by injecting Sr-85 microspheres into the nutrient artery, and (b) by infusing the short-lived radioactive gas, krypton-81m, thus producing an image of regional distribution of flow. Comparison of these profiles with the distribution of Tc-99m MDP shows that a moderate increase in flow is associated with a much greater increase in Tc-99m MDP residue. We conclude that, although blood flow is one determinant of the distribution of bone-seeking radionuclides such as Tc-99m MDP, some other determinants of clearance and residue explain their localization.

  9. The relationship between the percentage of lung shunting on Tc-99m macroaggregated albumin (Tc-99m MAA scan and the grade of hepatocellular carcinoma vascularity

    Directory of Open Access Journals (Sweden)

    Rania Refaat

    2014-06-01

    Conclusion: Tc-99m MAA scan is fundamental prior to Y-90 microsphere SIRT as it minimizes the risk of post-radioembolization complications, hence, enhancing the safety of Y-90 microsphere subsequent administration. Moreover, the percentage of lung shunting varies considerably among patients with HCC relying on the grade of tumor vascularity.

  10. [Use of a gamma probe for intraoperative localization of parathyroid adenomas with Tc-99m-tetrofosmin and Tc-99m-sestamibi].

    Science.gov (United States)

    Gallowitsch, H J; Fellinger, J; Kresnik, E; Mikosch, P; Pipam, W; Unterweger, O F; Lind, P

    1997-01-01

    Especially ectopic adenomas may be problematic in intraoperative localization despite preoperative imaging resulting in a longer operating time and more extensive surgical preparation. The aim of our study was to evaluate the possibility of intraoperative scintimetric detection of parathyroid adenomas with cationic, Tc-99m labelled tracers for its usefulness in ectopic adenomas. 12 women with biochemically confirmed hyperparathyroidism were included in our study. After injection of 370 MBq Tc-99m Tetrofosmin respectively Sestamibi, preoperative scintigraphy (double phase study and SPECT) was performed and T/NT ratios were evaluated for early, delayed and SPECT images. Surgery was performed using a hand-held gamma probe after preoperative injection of 555-925 MBq Tc-99m Tetrofosmin or Sestamibi. Count rates (cts/10 sec) were measured and used for calculating in situ- and ex situ-T/NT ratios. In 9 out of 12 patients, adenoma could be detected on static images. Three cases could only be detected with SPECT. 11 of 12 parathyroid adenomas could be confirmed intraoperatively. Intraoperative localization of parathyroid adenoma with Tc-99m-labelled cationic complexes and a gamma probe is possible and may be useful in case of ectopic adenoma by influencing surgical approach and operating time.

  11. Mapping lymph nodes in cancer management – role of 99mTc-tilmanocept injection

    Directory of Open Access Journals (Sweden)

    Tausch C

    2014-06-01

    Full Text Available Christoph Tausch, Astrid Baege, Christoph RagethBrust-Zentrum, Zürich, SwitzerlandAbstract: Two decades ago, lymphatic mapping of sentinel lymph nodes (SLN was introduced into surgical cancer management and was termed sentinel node navigated surgery. Although this technique is now routinely performed in the management of breast cancer and malignant melanoma, it is still under investigation for use in other cancers. The radioisotope technetium (99mTc and vital blue dyes are among the most widely used enhancers for SLN mapping, although near-infrared fluorescence imaging of indocyanine green is also becoming more commonly used. 99mTc-tilmanocept is a new synthetic radioisotope with a relatively small molecular size that was specifically developed for lymphatic mapping. Because of its small size, 99mTc-tilmanocept quickly migrates from its site of injection and rapidly accumulates in the SLN. The mannose moieties of 99mTc-tilmanosept facilitate its binding to mannose receptors (CD206 expressed in reticuloendothelial cells of the SLN. This binding prevents transit to second-echelon lymph nodes. In Phase III trials of breast cancer and malignant melanoma, and Phase II trials of other malignancies, 99mTc-tilmanocept had superior identification rates and sensitivity compared with blue dye. Trials comparing 99mTc-tilmanocept with other 99mTc-based agents are required before it can be routinely used in clinical settings.Keywords: lymphatic mapping, sentinel lymph node, new tracer

  12. Direct 99mTc labeling of monoclonal antibodies: radiolabeling and in vitro stability.

    Science.gov (United States)

    Garron, J Y; Moinereau, M; Pasqualini, R; Saccavini, J C

    1991-01-01

    Direct labeling involves 99mTc binding to different donor groups on the protein, giving multiple binding sites of various affinities resulting in an in vivo instability. The stability has been considerably improved by activating the antibody using a controlled reduction reaction (using 2-aminoethanethiol). This reaction generates sulfhydryl groups, which are known to strongly bind 99mTc. The direct 99mTc antibody labeling method was explored using whole antibodies and fragments. Analytical methods were developed for routine evaluation of radiolabeling yield and in vitro stability. Stable direct antibody labeling with 99mTc requires the generation of sulfhydryl groups, which show high affinity binding sites for 99mTc. Such groups are obtained with 2-aminoethanethiol (AET), which induces the reduction of the intrachain or interchain disulfide bond, with no structural deterioration or any loss of immunobiological activity of the antibody. The development of fast, reliable analytical methods has made possible the qualitative and quantitative assessment of technetium species generated by the radiolabeling process. Labeling stability is determined by competition of the 99mTc-antibody bond with three ligands, Chelex 100 (a metal chelate-type resin), free DTPA solution and 1% HSA solution. Very good 99mTc-antibody stability is obtained with activated IgG (IgGa) and Fab' fragment, which makes these substances possible candidates for immunoscintigraphy use.

  13. Tumor scintigraphy by the method for subtracting the initial image with technetium-99m labeled antibody

    Energy Technology Data Exchange (ETDEWEB)

    Karube, Yoshiharu; Katsuno, Kentaro; Ito, Sanae; Matsunaga, Kazuhisa; Takata, Jiro [Fukuoka Univ. (Japan). Faculty of Pharmaceutical Sciences; Kuroki, Masahide; Murakami, Masaaki; Matsuoka, Yuji

    1999-12-01

    The method for subtracting the initial image from the localization image was evaluated for radioimmunoscintigraphy of tumors with technetium-99m (Tc-99m) labeled antibodies. Monoclonal antibodies were parental mouse and mouse-human chimeric antibodies to carcinoembryonic antigen (CEA), designated F11-39 and ChF11-39, respectively, both of which have been found to discriminate CEA in tumor tissues from the CEA-related antigens. After reduction of the intrinsic disulfide bonds, these antibodies were labeled with Tc-99m. In vivo studies were performed on athymic nude mice bearing the human CEA-producing gastric carcinoma xenografts. Though biodistribution results showed selective and progressive accumulation of Tc-99m labeled antibodies at the tumor site, high radioactivity in blood was inappropriate for scintigraphic visualization of the tumors within a few hours. We examined the subtraction of the initial Tc-99m image from the Tc-99m localization image after a few hours. Subtracted images of the same count reflected the in vivo behavior of the Tc-99m radioactivity. The subtracted scintigrams revealed excellent tumor images with no significant extrarenal background. Visualization of the tumor site was dependent on antigen-specific binding and nonspecific exudation. These results demonstrate that a method of subtraction of the initial image may serve as a potentially useful diagnostic method for an abnormal site for agents with a low pharmacokinetic value. (author)

  14. A new approach for manufacturing and processing targets to produce 99mTc with cyclotrons

    Science.gov (United States)

    Matei, L.; McRae, G.; Galea, R.; Niculae, D.; Craciun, L.; Leonte, R.; Surette, G.; Langille, S.; Louis, C. St.; Gelbart, W.; Abeysekera, B.; Johnson, R. R.

    2017-06-01

    The most important radioisotope for nuclear medicine is 99mTc. After the supply crisis of 99Mo starting in 2008, the availability of 99mTc became a worldwide concern. Alternative methods for producing the medical imaging isotope 99mTc are actively being developed around the world. The reaction 100Mo(p, 2n)99mTc provides a direct route that can be incorporated into routine production in nuclear medicine centers that possess medical cyclotrons for production of other isotopes, such as those used for Positron Emission Tomography. This paper describes a new approach for manufacturing targets for the (p, 2n) nuclear reaction on 100Mo and the foundation for the subsequent commercial separation and purification of the 99mTc produced. Two designs of targets are presented. The targets used to produce 99mTc are subject to a number of operational constraints.They must withstand the temperatures generated by the irradiation, accommodate temperature gradients from cooling system of the target, must be resilient and must be easily post-processed to separate the 99mTc. After irradiation, the separation of Tc from Mo was carried out using an innovative two-step approach. The process described in this paper can be automated with modules that easily fit in standard production hot cells found in nuclear medicine facilities.

  15. A solvent-extraction module for cyclotron production of high-purity technetium-99m.

    Science.gov (United States)

    Martini, Petra; Boschi, Alessandra; Cicoria, Gianfranco; Uccelli, Licia; Pasquali, Micòl; Duatti, Adriano; Pupillo, Gaia; Marengo, Mario; Loriggiola, Massimo; Esposito, Juan

    2016-12-01

    The design and fabrication of a fully-automated, remotely controlled module for the extraction and purification of technetium-99m (Tc-99m), produced by proton bombardment of enriched Mo-100 molybdenum metallic targets in a low-energy medical cyclotron, is here described. After dissolution of the irradiated solid target in hydrogen peroxide, Tc-99m was obtained under the chemical form of (99m)TcO4(-), in high radionuclidic and radiochemical purity, by solvent extraction with methyl ethyl ketone (MEK). The extraction process was accomplished inside a glass column-shaped vial especially designed to allow for an easy automation of the whole procedure. Recovery yields were always >90% of the loaded activity. The final pertechnetate saline solution Na(99m)TcO4, purified using the automated module here described, is within the Pharmacopoeia quality control parameters and is therefore a valid alternative to generator-produced (99m)Tc. The resulting automated module is cost-effective and easily replicable for in-house production of high-purity Tc-99m by cyclotrons.

  16. 肺肿瘤99mTc-MDP显像研究%Study of 99mTc-MDP Imaging in Diagnosis of Lung Neoplasms

    Institute of Scientific and Technical Information of China (English)

    谢昌辉; 马志海; 李秀江; 张玉萍; 高凤彤

    2004-01-01

    目的探讨99mTc-羟基亚甲基二磷酸(MDP)显像鉴别诊断肺肿瘤的机制和临床价值.方法对63例经病理证实的肺肿瘤患者和10例正常对照进行早期10min99mTc-MDP显像,用感兴趣区分析法计算肿瘤和正常肺组织的放射性比(T/NT),探讨肺肿瘤病灶早期摄取99mTc-MDP的机制.结果肺癌的T/NT值(2.36±0.56)明显高于良性病灶(1.44±0.47)和正常对照(0.98±0.12)(t=5.26,7.70,P<0.01);以摄取比值>1.8为判断标准,99mTc-MDP显像诊断肺癌的灵敏度、特异性和准确率分别为88.2%、86.3%和86.7%;肺癌组织内残留的宿主血管、大量的新生血管和病变周围的血管集束为高水平99mTc-MDP血流灌注的特征性病理结构,肺癌的局部浸润转移促进了99mTc-MDP的摄取.结论99mTc-MDP的早期摄取与肺肿瘤的血供和局部浸润转移密切相关,对肺肿瘤的鉴别诊断有一定的临床价值.

  17. 99mTc-MIBI进行鼻咽癌显像的临床研究%Detection of nasopharyngeal carcinoma with Tc-99m MIBI

    Institute of Scientific and Technical Information of China (English)

    杜建强; 裴庆华; 岳殿超; 曾世荃

    2004-01-01

    Objective It is difficult to differentiate primary and recurrent nasopharyngeal carcinoma (NPC). Using 99mTc MIBI scintigraphy, we study patients with NPC in order to determine the efticacy of 99mTc MIBI scintigraphy in detecting the primary, recurrent and metastatic tumors. Method NPC patients were divided into two groups, primary (n= 43) and recurrent (n= 17). 99mTc MIBI SPECT study of head and neck and whole body scan to detect the primary, recurrent and metastases foci were performed on both groups as well as on volunteers (n = 21). Comparing with the scalp, the uptakes on the nasopharynx were divided into 5 grades, assessed by 3 physicians independently without any clinical information. Results In the primary NPC group, 36 patients were positive and 5 were negative. 13 patients had metastases in neck lymph nodes and 2 had lungs metastases. The specificity was 90.5 % ( 19 of 21) and the sensitivity was 82.7 % (36 of 43 ). In the recurrent group, 13 patients were positive, and 4 were negative. The sensitivity was 76.5 % ( 13 of 17 ). Conclusion This study suggests that 99mTc MIBI SPECT is a useful method for detecting NPC with particular potential value in diagnosis of recurrent NPC.%目的在临床上,鼻咽癌复发的诊断是非常困难的,我们用99mTc-MIBI来探索鼻咽癌的原发灶、复发灶和转移灶的诊断.方法用99mTc-MIBI断层显像检测21例对照组和41例治疗前鼻咽癌病例,病灶的放射性摄取与头皮比较,由3名以上的医师在不知道任何临床情况下分别阅片.结果41例治疗前鼻咽癌病例中共有36例阳性,特异性为90.5%,灵敏度为83.7%.结论99mTc-MIBI显像对鼻咽癌原发灶和转移灶的诊断,是一种灵敏的方法,值得进一步探讨.

  18. Indications for pediatric {sup 99m}Tc-dimercaptosuccinic-scintigraphy; Indikationen fuer die Szintigraphie mit {sup 99m}Tc-Dimercaptosuccinid in der Paediatrie

    Energy Technology Data Exchange (ETDEWEB)

    Meller, J.; Becker, W. [Goettingen Univ. (Germany). Abt. fuer Nuklearmedizin; Zappel, H. [Goettingen Univ. (Germany). Abt. fuer Paediatrische Kardiologie

    2000-11-01

    This paper gives an overview about the diagnostic use of {sup 99m}Tc-Dimercaptosuccinic ({sup 99m}Tc-DMSA)-scintigraphy in siblings and children and presents our own results with this tracer in pediatric nephro-urology. {sup 99m}Tc-DMSA offers a high renal accumulation and therefore is suited for the diagnosis of renal malformations. Furthermore, a calculation of the tubular renal split function and imaging of the renal cortex is possible due its high tubular retention. The tracer may be used with success in the non invasive diagnostic work up of both renoparenchymal and renovascular hypertension in childhood and especially in the diagnosis of segmental renal artery stenosis. Scintigraphy with {sup 99m}Tc-DMSA is a simple and highly effective method in the diagnosis of pyelonephritis and renal scarring. It offers important informations about the further prognosis of a child with urinary tract infection. In upper urinary tract infection {sup 99m}Tc-DMSA-scintigraphy may be more specific than available clinical tests. In the diagnosis of pyelonephritis and renal scarring scintigraphic imaging has been proven to be more sensitive than pyelography and ultrasound and its diagnostic power is at least equal compared with computed tomography. Therefore, imaging with {sup 99m}Tc-DMSA can be considered as the reference method in these questions. Regarding that reflux is seen in less than 40% of children with a pathologic DMSA-scan and that the prognosis of children with an urinary tract infection without a pathologic DMSA-scan is usually good, one could question the use of micturating cystourethrography in the diagnostic work up of children with symptomatic urinary tract infection and a normal DMSA-scan. (orig.) [German] Die vorliegende Arbeit gibt einen Ueberblick ueber die Verwendungsmoeglichkeiten der {sup 99m}Tc-Dimercaptosuccinid ({sup 99m}Tc-DMSA)-Szintigraphie im Saeuglings- und Kindesalter und moechte in diesem Rahmen auch ueber eigene Ergebnisse bei

  19. Novel 99mTc labeled σ receptor ligand as a potential tumor imaging agent

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A novel 99mTc labeled complex, [N-[2-((2-oxo-2-(4-(3-phenylpropyl)piperazin-1-yl)ethyl) (2-mercaptoethyl)amino)acetyl]-2-aminoethanethiolato]Technetium(V) oxide (PPPE-MAMA′-99mTcO) ([99mTc]-2) has been designed and prepared based on the integrated approach. The corresponding rhenium complex (PPPE-MAMA′-ReO)(Re-2) has been prepared and characterized. In vitro competition binding assays show moderate affinity of Re-2 towards σ1 and σ2 receptors with Ki values of 8.67 ± 0.07 and 5.71 ± 1.88 μmol, respectively. Planar images obtained at 0.5 h, 4 h, 20 h after I.v. Injection indicate the accumulation of [99mTc]-2 in MCF-7 human breast tumor bearing mice at 20 h. Furthermore, the accumulation of [99mTc]-2 has been inhibited at 20 h after co-injection of [99mTc]-2 plus haloperidol (1 mg/kg). Biodistribution studies of [99mTc]-2 display an in vivo tumor uptake of 0.14% ± 0.01% ID/g at 24 h post I.v. Injection with a tumor/muscle ratio of 6.02 ± 0.87. The above results suggest that [99mTc]-2, derived from a previously published lead compound, retains certain tumor uptake and affinity for σ receptors. [99mTc]-2 may be used as a basis for further structural modifications to develop tumor imaging agents with high affinity for σ receptors.

  20. Simulation of the direct production of {sup 99m}Tc at a small cyclotron

    Energy Technology Data Exchange (ETDEWEB)

    Eslami, M., E-mail: emohammad@znu.ac.ir [Department of Physics, Faculty of Science, Zanjan University, Zǝngan (Zanjan) (Iran, Islamic Republic of); Kakavand, T. [Department of Physics, Faculty of Science, Imam Khomeini International University, Qazvin (Iran, Islamic Republic of)

    2014-06-01

    Highlights: • The production yield was estimated for {sup 98,100,nat}Mo(p,x){sup 99m}Tc nuclear reactions. • MCNPX was used to calculate the proton energy distribution and secondary neutron flux. • The residual nuclei during the reactions were monitored as an alternative method. • Good agreement was obtained between experimental, theoretical and simulation-based yields. - Abstract: Usually {sup 99m}Tc is produced indirectly through generator {sup 99}Mo/{sup 99m}Tc. In the present study, the direct production of this radioisotope by charged particle irradiation was investigated using Monte Carlo method. After scouting of the reactions that produce {sup 99m}Tc, excitation functions of these reactions were predicted by optical model components in the TALYS-1.6 code. Suitable energy range of projectile for this production was selected by spotting of maximum cross section and minimum impurity due to other emission channels. Then target geometry was designed based on stopping power calculation by the SRIM code. Thick target yield of {sup 100}Mo(p,2n){sup 99m}Tc, {sup 98}Mo(p,γ){sup 99m}Tc and {sup nat}Mo(p,x){sup 99m}Tc reactions was predicted by the result of excitation function and stopping power calculations. Finally, {sup 100}Mo(p,2n){sup 99m}Tc reaction was selected as a primary reaction for the direct production of {sup 99m}Tc and its process was simulated by employing the MCNPX code to calculate the energy distribution of proton in the {sup 100}Mo target body and estimation of residual nuclei during irradiation. Good agreement was obtained between the experimental, the theoretical, and the simulation-based (analytical and directly) production yields. This study demonstrated that Monte Carlo provides a method for the design and optimization of targets for the radionuclide production purposes.

  1. 99Mo/(99m)Tc separation: an assessment of technology options.

    Science.gov (United States)

    Dash, Ashutosh; Knapp, F F Russ; Pillai, M R A

    2013-02-01

    Several strategies for the effective separation of (99m)Tc from (99)Mo have been developed and validated. Due to the success of column chromatographic separation using acidic alumina coupled with high specific activity fission (99)Mo (F (99)Mo) for production of (99)Mo/(99m)Tc generators, however, most technologies until recently have generated little interest. The reduced availability of F (99)Mo and consequently the shortage of (99)Mo/(99m)Tc column generators in the recent past have resurrected interest in the production of (99)Mo as well as (99m)Tc by alternate routes. Most of these alternative production processes require separation techniques capable of providing clinical grade (99m)Tc from low specific activity (99)Mo or irradiated Mo targets. For this reason there has been renewed interest in alternate separation routes. This paper reviews the reported separation technologies which include column chromatography, solvent extraction, sublimation and gel systems that have been traditionally used for the fabrication of (99)Mo/(99m)Tc generator systems. The comparative advantage, disadvantage, and technical challenges toward adapting the emerging requirements are discussed. New developments such as solid-phase column extraction, electrochemical separation, extraction chromatography, supported liquid membrane (SLM) and thermochromatographic techniques are also being evaluated for their potential application in the changed scenario of providing (99m)Tc from alternate routes. Based on the analysis provided in this review, it appears that some proven separation technologies can be quickly resurrected for the separation of clinical grade (99m)Tc from macroscopic levels of reactor or cyclotron irradiated molybdenum targets. Furthermore, emerging technologies can be developed further to respond to the expected changing modes of (99m)Tc production.

  2. Synthesis, biodistribution and imaging of {sup 99m}tc-7-HYNIC-TAXOL

    Energy Technology Data Exchange (ETDEWEB)

    Rha, D. S.; Park, J. Y.; Awh, O. D. [College of Health Science, Yonsei University, Seoul (Korea, Republic of); Lee, T. S.; Choi, T. H.; Woo, K. S.; Chung, W. S.; Choi, C. W.; Kim, S. M. [Korea Institutes of Radiological and Medical Sciences, Seoul (Korea, Republic of)

    2005-07-01

    Taxol has been used in the treatment of breast, ovary and lung cancers. To evaluate the feasibility of 99mTc-7-HYNIC(hydrazino nicotinamide)-taxol as a tumor imaging agent, it was synthesized, and its biodistribution and gamma camera image were obtained in B16-F10 melanoma bearing C57BL6 mice 7-t-BOC-HYNIC-taxol was synthesized through six steps, and 7-HYNIC-taxol was finally obtained by t-BOC deprotecting from 7-t-BOC-HYNIC-taxol. The product was purified by column chromatography. 99mTc-7-HYNIC-taxol complex from 7-HYNIC-taxol was prepared by labeling with 99mTc in the presence of SnCl2{center_dot}2H20 and tricine. The biochemical behaviors of the complex such as in vitro stability and lipophilicity, in vitro transchelation were investigated. The biodistribution and in vivo image of 99mTc-7-HYNIC-taxol were obtained in B16-F10 melanoma bearing C57BL6 mice. After 1, 6 and 24 hr post-injection, the weight and radioactivity of each organ were measured and gamma camera image was obtained. The total synthetic yield of 7-HYNIC-taxol was 42.6%. Radiolabeling yield of 99mTc-HYNIC-taxol was 99.9%. 99mTc-7-HYNIC-taxol was stable at 37? for 24 hrs. 99mTc-7-HYNIC-taxol was slightly more soluble in water than in organic solvent. The binding ability of 99mTc-7-HYNIC-taxol to serum proteins was 39.9%. In vivo transchelation test, the 99mTc-7-HYNIC-taxol retained over 86% of radiochemical purity after incubation with DTPA or cysteine. 99mTc-7-HYNIC-taxol was intravenously administered to C57BL6 mice bearing B16-F10 melanoma at footpad. Tumor/blood ratios were 1.17, 26.0, and 2.87, and tumor/muscle ratios were 12.2, 168, and 15.0 at 1 h. 6 h and 24 h post injection, respectively. The gamma camera image was obtained at 6 h post injection showed selectively localized in tumor. 99mTc-7-HYNIC-taxol showed high stability and was selectively localized in B16-F10 melanoma. These results suggest that 99mTc-7-HYNIC-taxol can be used as tumor imaging agent.

  3. Leukocyte-technetium-99m uptake in Crohn s disease:Does it show subclinical disease?

    Institute of Scientific and Technical Information of China (English)

    Luciene; G; Mota; Luiz; GV; Coelho; Carlos; JR; Simal; Maria; LA; Ferrari; Clodomiro; Toledo; Josep; Martin-Comin; Simone; OF; Diniz; Valbert; N; Cardoso

    2010-01-01

    AIM:To evaluate inflammatory activity in patients with Crohn's disease (CD) using technetium-99m-hexamethylpropyleneamine oxime (99mTc-HMPAO) granulocyte scintigraphy.METHODS: Twenty patients (7 male and 13 female) with CD and five healthy volunteers were selected for 99mTc-HMPAO granulocyte scintigraphy. The Crohn's Disease Activity Index (CDAI), blood tests and C-reactive protein (CRP) of each patient were performed 7 d before the scintigraphic images. The leukocytes were labeled according to the Internat...

  4. 99mTc MIBI in neurofibromatosis imaging diagnosis: case report.

    Science.gov (United States)

    Stefănescu, C; Meignan, M; Volkenstein, P; Rusu, V

    1996-01-01

    99mTc MIBI has been shown to accumulate in different cancer cells types, in vitro and malignant tumours in vivo, making evidence of the scintigraphic diagnostic of them. We report two cases of neurofibromatosis type I, which has realised high levels of 99mTc MIBI uptake, primary to all chemo- or radiotherapy. Only certain zones of tumorous localisation have been seen, but a relation with the lesion histology was not really established. In conclusion, 99mTc MIBI scintigraphy may be usefull in the investigation of type I neurofibromatosis.

  5. In vitro study: binding of 99mTc-DPD to synthetic amyloid fibrils

    Directory of Open Access Journals (Sweden)

    Buroni Federica E

    2015-12-01

    Full Text Available This paper is an report of the investigation of the in vitro binding of 99mTc-DPD for synthetic amyloid fibrils used for the diagnosis of cardiac amyloidosis (CA, as compared with the use of 99mTc-HMDP and 99mTc-PPI. It also includes an inquiry into the role played by Ca2+ ions and serum proteins on binding to amyloid like materials, as well as the saturability and specificity of DPD for fibrils versus amorphous precipitates (AP.

  6. A case of hepatopulmonary syndrome diagnosed by {sup 99m}Tc-MAA perfusion lung scan

    Energy Technology Data Exchange (ETDEWEB)

    Oh, Hyung Tae; Lee, Moo Yong; Song, Il Han; Park, Seok Gun [College of Medicine, Dankook Univ., Cheonan (Korea, Republic of)

    2002-06-01

    In patients with chronic liver disease, hepatopulmonary syndrome, the right to left shunt without previous pulmonary and cardiac disease, can develope and cause respiratory distress. Tools to prove shunt are contrast echocardiography, pulmonary angiography, and {sup 99m}Tc-MAA perfusion lung scan. Among them, {sup 99m}Tc-MAA scan is a simple and safe method detecting the right to left shunt. At the same time, quantitation of shunt amount is possible by this method. We report a case of hepatopulmonary syndrome confirmed by {sup 99m}Tc-MAA scan and contrast echocardiography with review of literatures.

  7. A conveniently prepared Tc-99m resin for semisolid gastric emptying studies

    Energy Technology Data Exchange (ETDEWEB)

    Wirth, N.; Swanson, D.; Shapiro, B.; Nakajo, M.; Coffey, J.L.; Eckhauser, F.; Owyang, C.

    1983-06-01

    A polystyrene resin, suitable for semi-solid gastric emptying studies, was rapidly (less than 20 min) and conveniently prepared using commercially available reagents. Using the outlined procedure, Chelex-100 resin bound Tc-99m with greater than 98% labeling efficiency. The resulting Tc-99m Chelex-100 resin demonstrated excellent in vitro and in vivo stability. The clinical application of Tc-99m Chelex-100 resin, mixed with oatmeal, was tested in normal subjects and in various patient groups, including diabetic autonomic neuropathy, pyloric obstruction, postoperative dumping syndrome, and morbidly obese patients before and after gastroplasty.

  8. Conveniently prepared Tc-99m resin for semisolid gastric emptying studies

    Energy Technology Data Exchange (ETDEWEB)

    Wirth, N. (Univ. of Michigan, Ann Arbor); Shapiro, B.; Nakajo, M.; Coffey, J.L.; Eckhauser, F.; Owyang, C.

    1983-06-01

    A polystyrene resin, suitable for semi-solid gastric emptying studies, was rapidly (<20 min) and coveniently prepared using commercially available reagents. Using the outlined procedure, Chelex-100 resin bound Tc-99m with greater than 98% labeling efficiency. The resulting Tc-99m Chelex-100 resin demonstrated excellent in vitro and in vivo stability. The clinical application of Tc-99m Chelex-100 resin, mixed with oatmeal, was tested in normal subjects and in various patient groups, including diabetic autonomic neuropathy, pyloric obstruction, postoperative dumping syndrome, and morbidly obese patients before and after gastroplasty.

  9. In vivo detection of multidrug-resistant (MDR1) phenotype by technetium-99m sestamibi scan in untreated breast cancer patients

    Energy Technology Data Exchange (ETDEWEB)

    Del Vecchio, S. [Medicina Nucleare, Istituto di Scienze Radiologiche, Universita degli Studi `Federico II`, Naples (Italy)]|[Centro per lo Studio della Medicina Nucleare, CNR, Naples (Italy); Ciarmiello, A. [Istituto Nazionale per lo Studio e la Cura dei Tumori, Via M. Semmola, Naples (Italy); Potena, M.I. [Istituto Nazionale per lo Studio e la Cura dei Tumori, Via M. Semmola, Naples (Italy); Carriero, M.V. [Istituto Nazionale per lo Studio e la Cura dei Tumori, Via M. Semmola, Naples (Italy); Mainolfi, C. [Medicina Nucleare, Istituto di Scienze Radiologiche, Universita degli Studi `Federico II`, Naples (Italy)]|[Centro per lo Studio della Medicina Nucleare, CNR, Naples (Italy); Botti, G. [Istituto Nazionale per lo Studio e la Cura dei Tumori, Via M. Semmola, Naples (Italy); Thomas, R. [Istituto Nazionale per lo Studio e la Cura dei Tumori, Via M. Semmola, Naples (Italy); Cerra, M. [Istituto Nazionale per lo Studio e la Cura dei Tumori, Via M. Semmola, Naples (Italy); D`Aiuto, G. [Istituto Nazionale per lo Studio e la Cura dei Tumori, Via M. Semmola, Naples (Italy); Tsuruo, T. [Institute of Applied Microbiology, University of Tokyo, Bunkyo-Ku, Tokyo 113 (Japan); Salvatore, M. [Medicina Nucleare, Istituto di Scienze Radiologiche, Universita degli Studi `Federico II`, Naples (Italy)]|[Centro per lo Studio della Medicina Nucleare, CNR, Naples (Italy)

    1997-02-01

    Technetium-99m sestamibi is a transport substrate recognised by the multidrug-resistant P-glycoprotein (Pgp). To test whether {sup 99m}Tc-sestamibi efflux is enhanced in breast carcinomas overexpressing Pgp, we determined the efflux rates of {sup 99m}Tc-sestamibi and Pgp levels in tumours from 30 patients with untreated breast carcinoma. Patients were intravenously injected with 740 MBq of {sup 99m}Tc-sestamibi and underwent a 15-min dynamic study followed by the acquisition of static planar images at 0.5, 1, 2 and 4 h. Tumour specimens were obtained from each patient 24 h after {sup 99m}Tc-sestamibi scan and Pgp levels were determined using {sup 125}I-MRK16 monoclonal antibody and in vitro quantitative autoradiography. All breast carcinomas showed high uptake of {sup 99m}Tc-sestamibi and data from region of interest analysis on sequential images were fitted with a monoexponential function. The efflux rates of {sup 99m}Tc-sestamibi, calculated from decay-corrected time-activity curves, ranged between 0.00121 and 0.01690 min{sup -1}and were directly correlated with Pgp levels measured in the same tumours (r=0.62; P <0.001). Ten out of 30 breast carcinomas (33%) contained 5 times more Pgp than benign breast lesions and showed a mean concentration of 5.73 {+-} 1.63 pmol/g of tumour (group A). The remaining 20 breast carcinomas had a mean Pgp concentration of 1.29 {+-}0.64 pmol/g (group B), equivalent to that found in benign breast lesions. {sup 99m}Tc-sestamibi efflux from tumours of group A was 2.7 times higher than that observed in tumours of group B (0.00686 {+-}0.00390 min {sup -1}vs 0.00250 {+-}0.00090 min {sup -1}, P <0.001). The in vivo functional test with {sup 99m}Tc-sestamibi showed a sensitivity and a specificity of 80% and 95%, respectively. In conclusion, the efflux rate of {sup 99m}Tc-sestamibi may be used for the in vivo identification of the multidrug resistant (MDR1) phenotype in untreated breast cancer patients. (orig.). With 7 figs., 3 tabs.

  10. [Protein losing enteropathy (PLE) detected by Tc99m-labelled human serum albumin abdominal scintigraphy--case report].

    Science.gov (United States)

    Hubalewska-Hoła, Alicja; Sowa-Staszczak, Anna; Szczerbiński, Tomasz; Lis, Grzegorz; Huszno, Bohdan; Szybiński, Zbigniew

    2003-01-01

    Protein losing enteropathy (PLE) is a gastrointestinal disorder that is associated with excessive loss of plasma protein into the gut resulting from abnormal mucosal permeability. The disease is usually caused by inflammation. The loss of protein in PLE is a nonselective process affecting albumin, globulin and transferrin. Abdominal scintigraphy with human serum albumin marked by Tc99m seems to be an easy and sensitive method for diagnosing PLE. An 4-year-old girl was presented to an outside Pediatric Department due to hypoproteinemia and recurrent pneumonia which had caused several prior hospitalizations. The laboratory tests revealed hypoproteinemia, hypoalbuminemia, low level of IgG, sideropenia, and a decreased level of T lymphocytes. The loss of protein into the gut was confirmed by fecal clearance of alfa-1 antitrypsin. Only nonspecific inflammation was detected by biopsy of the small intestine. These clinical and laboratory findings, quickly decreasing IgG and albumin levels in spite of i.v. supplementation and the lack of proteinuria permitted PLE diagnosis. The abdominal scintigraphy was planned to assess and localise protein losing through GIT and for strategy of possible surgical treatment. Abdominal dynamic scintigraphy was performed immediately after the injection of 300 MBq Tc99m human albumin. 90 images were taken within 180 minutes. Delayed abdominal images were obtained 6 and 24 hours after the tracer injection. Anterior abdominal scintigraphy showed pathological activity of Tc99m-albumin in small bowel in the upper left segment of the abdomen in the 40th minute after injection. Extensive accumulation of albumin was seen in the 160th minute. Delayed images, after 3 and 6 hours, revealed translocation of the tracer into the lower right abdominal segment. The further passage and tracer concentration was detected in ascendant and transverse colon. Based on the laboratory tests and scintigraphic images the girl was suspected to have segmental

  11. The diagnostic value of 11C-methionine PET in hyperparathyroidism with negative 99mTc-MIBI SPECT: a meta-analysis.

    Science.gov (United States)

    Yuan, Leilei; Liu, Jun; Kan, Ying; Yang, Jigang; Wang, Xufu

    2017-05-01

    Background 99mTc-sestamibi (MIBI) parathyroid SPECT is generally regarded as the best preoperative localizing method in patients with hyperparathyroidism (HPT). However, 99mTc-MIBI SPECT is false negative in approximately 25% of adenomas. 11C-methionine positron emission tomography (PET) has been used in HPT with negative 99mTc-MIBI SPECT scan results. Purpose To systematically review and conduct a meta-analysis of published data on the performance of 11C-methionine PET in patients with HPT with negative 99mTc-MIBI SPECT. Material and Methods A comprehensive review of the literature was performed. Pooled sensitivity and specificity of 11C-methionine PET in patients with HPT and a negative 99mTc-MIBI SPECT was calculated on a per-patient basis using receiver-operating characteristic (ROC) methodology. Results Nine studies that met all inclusion and exclusion criteria were included into our meta-analysis, comprising a total sample size of 137 patients. Pooled sensitivity and specificity of 11C-methionine PET in patients with HPT with negative or inconclusive 99mTc-MIBI SPECT scans was 86% and 86%, respectively. The area under the ROC curve was 0.87. Conclusion By merit of the high overall sensitivity, specificity, and accuracy, 11C-methionine PET can potentially complement the diagnostic workup of patients with HPT and negative or inconclusive 99mTc-MIBI SPECT. 11C-methionine PET appears to be a promising diagnostic modality in complicated cases with HPT.

  12. Synthesis and evaluation of a technetium-99m labeled cytotoxic bombesin peptide conjugate for targeting bombesin receptor-expressing tumors

    Energy Technology Data Exchange (ETDEWEB)

    Okarvi, Subhani M. [Cyclotron and Radiopharmaceuticals Department, King Faisal Specialist Hospital and Research Centre, MBC-03, PO Box 3354, Riyadh 11211 (Saudi Arabia)], E-mail: sokarvi@kfshrc.edu.sa; Al Jammaz, Ibrahim [Cyclotron and Radiopharmaceuticals Department, King Faisal Specialist Hospital and Research Centre, MBC-03, PO Box 3354, Riyadh 11211 (Saudi Arabia)

    2010-04-15

    Conjugation of the cytotoxic drugs to receptor-binding peptides is an attractive approach for the targeted delivery of cytotoxic peptide conjugates to tumor cells. In an attempt to develop an efficient peptide-based radiopharmaceutical for targeting bombesin (BN) receptor-expressing tumors (i.e., breast and prostate), we have prepared by solid-phase peptide synthesis, a novel BN analog derived from the universal sequence of BN and conjugated to a widely characterized antineoplastic agent, methotrexate (MTX). MTX-BN, after radiolabeling with {sup 99m}Tc via stannous-tartrate exchange, showed a good stability against cysteine and histidine transchelation as well as a high in vitro metabolic stability in human plasma. In vitro cell-binding and internalization on MDA-MB-231, MCF-7, T47-D breast cancer and PC-3 prostate cancer cell lines demonstrated high affinity and specificity of {sup 99m}Tc-MTX-BN towards both human breast and prostate cancer cells (binding affinities in nanomolar range). In addition, the radioconjugate displayed a significant internalization (values ranged between 19-35%) into the tumor cells. In vivo biodistribution and clearance kinetics in Balb/c mice are characterized by an efficient clearance from the blood and excretion mainly through the renal-urinary pathway with some elimination via the hepatobiliary system. In vivo tumor uptake in nude mice bearing MDA-MB-231 cells was 2.70{+-}0.44% ID/g at 1 h, whereas in nude mice with human epidermoid KB cells the accumulation in the tumor was found to be 1.48{+-}0.31% ID/g at 1 h post injection. The tumor uptake was always higher than in the blood and muscle, with good tumor retention and good tumor-to-blood and tumor-to-muscle ratios. The accumulation/retention in the major organs (i.e., lungs, stomach, liver, intestines, etc.) was low to moderate (<6% ID/g) in both healthy and tumor-bearing mice. However, the uptake/retention in the kidneys was rather high (up to 11.05{+-}1.80% ID/g), which is of a

  13. Novel [99mTcN]2+ Labeled EGFR Inhibitors as Potential Radiotracers for Single Photon Emission Computed Tomography (SPECT Tumor Imaging

    Directory of Open Access Journals (Sweden)

    Mingxia Zhao

    2014-04-01

    Full Text Available The epidermal growth factor receptor (EGFR is overexpressed in many cancers, including breast, ovarian, endometrial and non-small cell lung cancer. An EGFR-specific imaging agent could facilitate clinical evaluation of primary tumors or metastases. To achieve this goal, 4-(2-aminoethylamino-6,7-dimethoxyquinazoline (ADMQ was synthesized based on a 4-aminoquinazoline core and then conjugated with N-mercapto- acetylglycine (MAG and N-mercaptoacetyltriglycine (MAG3, respectively, to give compounds 1 and 2. The final complexes [99mTcN]-1 and [99mTcN]-2 were successfully obtained with radiochemical purities of >99% and >98% as measured by radio-HPLC. No decomposition of the two complexes at room temperature was observed over a period of 2 h. Their partition coefficients indicated they were hydrophilic and the electrophoresis results showed they were negatively charged. Biodistribution in tumor-bearing mice demonstrated that the two new complexes showed tumor accumulation, high tumor-tomuscle (T/M ratios and fast clearance from blood and muscle. Between the two compounds, the 99mTcN-MAG3-ADMQ ([99mTcN]-2 showed the better characteristics, with the tumor/muscle and tumor/blood ratios reached 2.11 and 1.90 at 60 min post-injection, 4.20 and 1.10 at 120 min post-injection, suggesting it could be a promising radiotracer for SPECT tumor imaging.

  14. Synthesis and biological assessment of folate-accepted developer (99m)Tc-DTPA-folate-polymer.

    Science.gov (United States)

    Chen, Fei; Shao, Kejing; Zhu, Bao; Jiang, Mengjun

    2016-05-15

    A novel cancer-targetable folate-poly(2-hydroxyethyl methacrylate) (PFDH) copolymer containing DTPA segment was prepared by conventional chemical synthesis and labeled with (99m)Tc subsequently. The (99m)Tc-labled PFDH could be produced easily with high radiochemical yield of 91% and radiochemical purity of 95%. The LogP octanol-water value for the (99m)Tc-labled PFDH was -2.19 and the radiotracer was stable in phosphate-buffered saline and human serum for 2h (>95% in PBS or ∼90% in human serum). To investigate (99m)Tc-labled PFDH tumor targeting, the in vitro and in vivo stability, cell uptake, in vivo biodistribution, and SPECT imaging were evaluated, respectively. These preliminary results strongly suggest that the novel folate conjugated dendrimer maybe developed to be potential for delivery of therapeutic radionuclides.

  15. Tc-99m-MIBI Uptake in Active Pulmonary Tuberculosis: A Case Report

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Chang Ho; Park, Chan Hee; Hwang, Hee Sung; Bae, Moon Sun [Ajou University College of Medicine, Suwon (Korea, Republic of)

    1996-06-15

    Technetium-99m MIBI was developed as a myocardiac perfusion imagine agent and has been used effectively in the detection and post-therapeutic evaluation of various neoplasm such as thyroid, lung, bone and breast tumors. As an infrequent findings, Tc-99m MIBI agent has shown in non-neoplastic pulmonary conditions including fibroding alveolitis, pulmonary actinomycosis, active pulmonary sarcoidosis, pulmonary interstitial fibrosis in progressive systemic sclerosis and active osteomyelitis. In a recent report conducted by Cetin Oncel, Tc-99m MIBI imaging is an effective method in the detection and follow-up of pulmonary tuberculosis. We have also experienced Tc-99m-MIBI uptake in active pulmonary tuberculosis incidentally found in a patient with suspected proliferative villonodular synovitis of the left ankle.

  16. Normal appearances of technetium-99m dimercaptosuccinic acid in children on planar imaging

    Energy Technology Data Exchange (ETDEWEB)

    Pusuwan, P.; Reyes, L.; Gordon, I. [Department of Radiology, Hospital for Children NHS Trust, London (United Kingdom)

    1999-05-01

    Thirty-two children who underwent renal arteriography with normal results also underwent technetium-99m dimercaptosuccinic acid (DMSA) imaging. Variations in the normal appearance of {sup 99m}Tc-DMSA images are described in these children. Criteria for high-quality {sup 99m}Tc DMSA images are defined in terms of kidney outline and contrast between the inner and the outer part of the kidney. Most of the images in this study were of high quality. The two most common features of normal {sup 99m}Tc-DMSA images were a round-shaped (50%) or flat (either lateral or medial aspect) (24.8%) contour. A small number of unusual appearances were observed and these have been illustrated. The mean differential function of the left kidney was 51% with a range of 45%-57%. (orig.) With 9 figs., 3 tabs., 15 refs.

  17. Calibration of KRISS reference ionization chamber for key comparison of (99m)Tc measurement.

    Science.gov (United States)

    Lee, Jong-Man; Lee, K B; Lee, S H; Park, Tae Soon

    2012-09-01

    KRISS, as the national metrology institute of Korea, has used a reference ionization chamber system to certify the activity of (99m)Tc aqueous sources, but could only recently participate in a comparison exercise by the BIPM (BIPM.RI(II)-K4.Tc-99m) to secure the international equivalence of (99m)Tc radioactivity measurement by way of the BIPM transfer instrument (SIRTI). The KRISS ionization chamber system was calibrated about 100 days before the comparison with a (99m)Tc solution source standardized by the 4πβ(LS)-γ(NaI(Tl)) coincidence counting method. During the comparison, beginning with a higher activity mother solution, the KRISS ionization chamber measured its specific activity without a dilution. The activity of a diluted-solution source was measured by the SIRTI at the same time.

  18. Limitations and pitfalls of 99mTc-EDDA/HYNIC-TOC (Tektrotyd) scintigraphy.

    Science.gov (United States)

    Garai, Ildikó; Barna, Sandor; Nagy, Gabor; Forgacs, Attila

    2016-01-01

    Tektrotyd kit was developed by Polatom company for 99mTc labeling to make an alternative tracer of somatostatin receptor scintigraphy available. Since 2005, 99mTc-EDDA/HYNIC-Tyr3-Octreotide has been used in clinical imaging and achieved high impact in management of patients with neuroendocrine tumors. Knowing the limitations and pitfalls is essential to provide ac-curate diagnosis. Therefore, the potential pitfalls associated with the use of 99mTc-EDDA/HYNIC-TOC are reviewed on the basis of own experience. Data were analyzed of 310 patients who underwent somatostatin receptor scintigraphy with 99mTc-Tektrotyd. Pitfalls during radiolabeling process or acquisition can worsen the sensitivity of SRS (somatostatin receptor scintigraphy). Recognizing physi-ological and clinical pitfalls, the diagnostic accuracy will improve.

  19. .sup.100Mo compounds as accelerator targets for production of .sup.99mTc

    Science.gov (United States)

    Richards, Vernal; Lapi, Suzanne

    2016-09-20

    Methods of synthesizing .sup.100Mo.sub.2C and .sup.99mTcO.sub.4.sup.- are disclosed. Methods of .sup.100Mo.sub.2C generation involve thermally carburizing .sup.100MoO.sub.3. Methods of .sup.99mTcO.sub.4 generation involve proton bombardment of .sup.100Mo.sub.2C in a cyclotron. Yields of .sup.99mTcO.sub.4 can be increased by sintering .sup.100Mo.sub.2C prior to bombardment. The methods also include recycling of .sup.100Mo.sub.2C to form .sup.100MoO.sub.3. SPECT images obtained using .sup.99mTcO.sub.4 generated by the disclosed methods are also presented.

  20. Abnormal uptake of technetium-99m hexakis-2-methoxyisobutylisonitrile in a primary cardiac lymphoma

    Energy Technology Data Exchange (ETDEWEB)

    Medolago, G.; Virotta, G.; Bertocchi, C. (Ospedali Riuniti di Bergamo (Italy). Dept. of Nuclear Medicine); Piti, A.; Tespili, M.; D' Adda, F. (Ospedali Riuniti di Bergamo (Italy). Dept. of Cardiology); Rottoli, M.R. (Ospedali Riuniti di Bergamo (Italy). Dept. of Neurology); Comotti, B. (Ospedali Riuniti di Bergamo (Italy). Dept. of Hematology); Motta, T. (Ospedali Riuniti di Bergamo (Italy). Dept. of Pathology); Orlandi, C. (Du Pont Pharma, North Billerica, MA (United States))

    1992-03-01

    Abnormally high uptake of technetium-99m hexakis-2-methoxyisobutylisonitrile ({sup 99m}Tc-SESTAMIBI) in the right ventricle and in the septum was observed in a 47-year-old woman initially presenting with dysarthria and left hemiparesis. Endomyocardial biopsy demonstrated a high-grade malignant non-Hodgkin's lymphoma. Complete remission was achieved by combined cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP) chemotherpay and radiotherapy of the heart and mediastinum. The post-remission single photon emission tomography (SPET) {sup 99m}Tc-SESTAMIBI study showed a homogeneous distribution pattern, in agreement with echocardiography computed tomography and magnetic resonance imaging. Increased uptake of {sup 99m}Tc-SESTAMIBI, a myocardial perfusion agent, has been observed in some benign and malignant tumours. It may prove to be useful in the diagnosis and follow-up of malignancies. (orig.).

  1. Labelling, biodistribution and compartmental analysis of N-acetylcysteine labelled with Tc-99m. Comparative investigation with with {sup 99m} Tc-MIBI in an in vivo tumoral model; Estudo de marcacao, biodistribuicao e analise compartimental da N-acetil cisteina marcada com Tc-99m. Investigacao comparativa com MIBI-{sup 99m}Tc em modelo tumoral in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Faintuch, Bluma Linkowski

    1997-07-01

    Labelling and biodistribution studies were done with two different ligands, respectively Methoxy isobutyl isonitrile (MIBI) and N-acetylcysteine (NAC), employing Tc-99m as a tracer. The main objective was to assess the pharmacokinetic properties of the second substance, aiming at its possible application in cancer diagnosis. To this purpose an in vivo investigation was done using healthy and tumor-bearing rats with experimental cancer. Images of tumor-bearing rats registered in a scintillation camera indicated that with {sup 99m} Tc-MIBI none of the two selected times was adequate for visualization of the cancer mass. In contrast, {sup 99m} Tc-NAC permitted clear identification of the humor, four hours after injection. The results have demonstrated that {sup 99m} Tc-NAC is a radiopharmaceutical with affinity for cancer tissue and promising for further investigation concerning imaging diagnosis of tumors. (author)

  2. {sup 99m}Tc-besilesomab (Scintimun {sup registered}) in peripheral osteomyelitis: comparison with {sup 99m}Tc-labelled white blood cells

    Energy Technology Data Exchange (ETDEWEB)

    Richter, Wolf S. [Pharmtrace Klinische Entwicklung GmbH, Berlin (Germany); University Clinics Magdeburg (Germany). Clinics for Radiology and Nuclear Medicine; Ivancevic, Velimir [Nuclear Medicini, Celle (Germany); Meller, Johannes [University Medicine, Goettingen (Germany). Dept. of Nuclear Medicine; Lang, Otto [UH Kralovske, Prague (Czech Republic). Dept. of Nclear Medicine; Le Guludec, Dominique [CHU Bichat-Claude Bernard, Paris (France). Service de Medecine Nucleaire; Szilvazi, Istvan [Orszagos Gyogyintezeti Koezpont, Budapest (Hungary). Dept. of Nuclear Medicine; Amthauer, Holger [University Clinics Magdeburg (Germany). Clinics for Radiology and Nuclear Medicine; Chossat, Florence; Dahmane, Amel [IBA/CIS Bio International, Gif sur Yvette (France); Schwenke, Carsten [SCOSSIS, Berlin (Germany); Signore, Alberto [University of Rome, Faculty of Medicine, Nuclear Medicine Unit, Rome (Italy)

    2011-05-15

    The diagnosis of osteomyelitis is a challenge for diagnostic imaging. Nuclear medicine procedures including white blood cell imaging have been successfully used for the identification of bone infections. This multinational, phase III clinical study in 22 European centres was undertaken to compare anti-granulocyte imaging using the murine IgG antibody besilesomab (Scintimun {sup registered}) with {sup 99m}Tc-labelled white blood cells in patients with peripheral osteomyelitis. A total of 119 patients with suspected osteomyelitis of the peripheral skeleton received {sup 99m}Tc-besilesomab and {sup 99m}Tc-hexamethylpropyleneamine oxime (HMPAO)-labelled white blood cells (WBCs) in random order 2-4 days apart. Planar images were acquired at 4 and 24 h after injection. All scintigraphic images were interpreted in an off-site blinded read by three experienced physicians specialized in nuclear medicine, followed by a fourth blinded reader for adjudication. In addition, clinical follow-up information was collected and a final diagnosis was provided by the investigators and an independent truth panel. Safety data including levels of human anti-mouse antibodies (HAMA) and vital signs were recorded. The agreement in diagnosis across all three readers between Scintimun {sup registered} and {sup 99m}Tc-HMPAO-labelled WBCs was 0.83 (lower limit of the 95% confidence interval 0.8). Using the final diagnosis of the local investigator as a reference, Scintimun {sup registered} had higher sensitivity than {sup 99m}Tc-HMPAO-labelled WBCs (74.8 vs 59.0%) at slightly lower specificity (71.8 vs 79.5%, respectively). All parameters related to patient safety (laboratory data, vital signs) did not provide evidence of an elevated risk associated with the use of Scintimun {sup registered} except for two cases of transient hypotension. HAMA were detected in 16 of 116 patients after scan (13.8%). Scintimun {sup registered} imaging is accurate, efficacious and safe in the diagnosis of peripheral

  3. Evaluation of (99m)Tc(i)-tricarbonyl complexes of fluoroquinolones for targeting bacterial infection.

    Science.gov (United States)

    Nayak, Dipak Kumar; Baishya, Rinku; Halder, Kamal Krishna; Sen, Tuhinadri; Sarkar, Bharat R; Ganguly, Shantanu; Das, M K; Debnath, Mita Chatterjee

    2012-11-01

    The aim of this study was to develop (99m)Tc(CO)(3)-labeled fluoroquinolones as novel SPECT radiopharmaceuticals for imaging bacterial infection. Fluoroquinolones, e.g., ofloxacin (OFX), levofloxacin (LVX), lomefloxacin (LMX) and norfloxacin (NFX) were labeled with a fac-[(99m)Tc(CO)(3)(H(2)O)(3)](+) precursor. The radiochemical purity of the radiopharmaceuticals exceeded 97% as determined by thin layer chromatography and HPLC. No further purification was necessary before injection. The Re(CO)(3) complex of one of the fluoroquinolones (levofloxacin) was synthesized using [Re(CO)(3)(H(2)O)(3)]OTf and Re(CO)(5)Br precursors in separate experiments and characterized by IR, NMR and mass spectroscopic analysis. These studies revealed the formation of a single species in which the piperazinyl nitrogen and the -COOH group attached to the benzoxazine ring system of quinolone were involved in co-ordination to the Re(CO)(3) core. The HPLC elution pattern and retention time of the Re(CO)(3)-LVX complex were comparable to those of the corresponding (99m)Tc(CO)(3)-complex proving their similarity. When incubated in isotonic saline and serum up to 24 h (99m)Tc(CO)(3)-labeled fluoroquinolones exhibited good in vitro stability. Biodistribution studies performed at different time points on rats intramuscularly infected with S. aureus as well as on rats with sterile inflammation revealed a higher uptake in the infected area than the turpentine induced inflamed area. The uptake in infected thigh was significant with (99m)Tc(CO)(3)-OFX followed by (99m)Tc(CO)(3)-LVX. The mean ratios of the uptake in infected/non-infected thighs were 4.75 and 4.27 at 8 h and 24 h, respectively, for (99m)Tc(CO)(3)-OFX and 4.42 and 4.18 at 24 h and 8 h, respectively, for (99m)Tc(CO)(3)-LVX. The above abscess to muscle ratios were higher than reported for (99m)Tc-ciprofloxacin and other (99m)Tc-labeled fluoroquinolones. Scintigraphy studies also showed a significant uptake in the infectious lesions

  4. In vitro and in vivo studies with pentavalent technetium-99m dimercaptosuccinic acid

    Energy Technology Data Exchange (ETDEWEB)

    Lam, A.S.K. [Research School of Biosciences, Univ. of Kent, Canterbury (United Kingdom); Puncher, M.R.B. [Research School of Biosciences, Univ. of Kent, Canterbury (United Kingdom); Blower, P.J. [Research School of Biosciences, Univ. of Kent, Canterbury (United Kingdom)]|[Nuclear Medicine Department, Kent and Canterbury Hospital (United Kingdom)

    1996-12-01

    The purpose of this investigation was to characterise the in vivo chemistry and binding mecha- nisms of technetium-99m dimercaptosuccinic acid [{sup 99m}Tc(V)DMSA]. Biodistribution was studied in mice by frozen section whole-body autoradiography and microautoradiography in selected tissues. Binding to bone mineral analogues was studied in vitro using various forms of calcium phosphate and hydroxyapatite under varied conditions. Similar studies with {sup 99m}Tc-hydroxymethylene diphosphonate (HDP) were also carried out for comparison. The in vivo stability of {sup 99m}Tc(V)DMSA was monitored by high-performance liquid chromatographic analysis of blood and urine samples taken over 24 h from patients injected with the tracer. Whole-body autoradiography shows that {sup 99m}Tc(V)DMSA has highest affinity for bone (cortical rather than medullary) in mice. Substantial uptake of the tracer was also observed in the kidney (cytoplasm of cortical renal tubular cells). No specific localisation was observed in the liver at either the microscopic or the macroscopic level. While {sup 99m}Tc-HDP bound strongly to calcium phosphates under all conditions, {sup 99m}Tc(V)DMSA binding was inhibited in the presence of phosphate and was stronger at pH 6.0 than at pH 7.4. In non-phosphate buffers, however, the binding of {sup 99m}Tc(V)DMSA remained high across the pH range 4-7.4. {sup 99m}Tc(V)DMSA binds to calcium phosphates chemically unaltered, and no radioactive species other than the three isomers of {sup 99m}Tc(V)DMSA were detected in blood or urine samples taken from patients up to 24 h after injection. {sup 99m}Tc(V)DMSA is stable in vivo, and no conversion of the complex to other chemical species needs to be invoked to explain its uptake in bone metastases or soft tissue tumour. Bone affinity may be due to reversible binding of the unaltered complex to the mineral phase of bone. (orig.). With 6 figs., 1 tab.

  5. Human pharmacokinetics, biodistribution and dosimetry of the kit of monoclonal antibody IOR EGF/R3 labelled with {sup 99m} Tc

    Energy Technology Data Exchange (ETDEWEB)

    Torres, L.A.; Ramos, M.; Perera, A.; Hernandez, A.; Iznaga, M.E. N. [Solano, Ivette Alvarez, Jose L. Rodriguez. Centro de InvestigacionesClinicas. 34 no.4501 e/45 y 47 Kohly, Playa, C. Habana (Cuba)

    1998-12-31

    The aim of this work was to assess the human pharmacokinetics, biodistribution and dosimetry of the {sup 99m} Tc-labeled MAb ior egf/r3. Five patients were included in the biodistribution and dosimetric studies and three in the pharmacokinetic analysis. Multiple blood and urine samples we recollected and sequential anterior and posterior whole-body scintigraphies u pto 24 hr post-injection were performed to all patients . The internal radiation dosimetry was estimated from gamma camera imaging data using the methods developed by the Medical Internal radiation dosimetry (MIRD)committee. Raw data were computed from operations between gamma graphic images and regions of interest (ROI) using the Bio-Dose software and time-activity curves were calculated in order to determine the residence times of the source organs. The Pharmacokinetics and Biodistribution results showed that this compound have a bio exponential plasmatic and blood clearance with a rapid biodistribution phase of 9.1 {+-} 8.4 min and 12.2{+-}4.4 min, respectively, and a slower elimination phase of 6.6 {+-} 1.6 hr and 10.8 {+-} 6.8 hr. respectively. The urinary and hepatobiliary excretion showed 4.7 {+-} 0.4 % and 9.9 {+-} 1.8 % of the total administered dose,eliminated by these ways. Liver was the target organ of this product and had an uptake peak at 1 hr post-injection (61.2%) and a great retention of the MAb(T 1/2 eff = 5.3 hr, T 1/2 Biol. = 45.0 hr). The dosimetric results showed that liver, gallbladder and spleen received the higher absorbed. The effective dose and the effective equivalent dose were 1,2E-01 mSv/MBq and 9,2E-02 mSv/MBq respectively. These results allow to see the i or egf/r3 kit in a safe and controlled way. (Author)

  6. Intense splenic 99mTc-MDP uptake in a patient with myelofibrosis.

    Science.gov (United States)

    Chen, Ming; Liu, Chun; Yang, Jigang

    2013-12-01

    99mTc-MDP bone scan was performed in a 49-year-old woman with breast cancer. Whole-body bone scan showed multiple foci of increased MDP activity in the bone and intense splenic 99mTc-MDP uptake. Initial bone marrow aspiration in multiple locations yielded no blood cells. A subsequent bone marrow biopsy in the left anterior superior iliac spine showed myelofibrosis in addition to the known bone metastasis.

  7. Tc-99m Labeled HMPAO white Blood Cell Scintigraphy in Pediatric Patients

    OpenAIRE

    Funda Aydın3; Arzu Kın Cengiz; Fırat Güngör

    2012-01-01

    Objective: 99mTc labeled hexamethylpropylene amine oxime (HMPAO) white blood cell (WBC) scintigraphy is a frequently used option for acute infection, particularly in pediatric patients. This scintigraphy is applied to detect sites of infection/inflammation in patients with fever of unknown origin, to find and follow up osteomyelitis, and to detect suspicion of acute appendicitis. The aim of this retrospective study was to evaluate the value of 99mTc-HMPAO labeled WBC scintigraphy in pediatric...

  8. Dose calibrator linearity test: 99mTc versus 18F radioisotopes

    Directory of Open Access Journals (Sweden)

    José Willegaignon

    2015-02-01

    Full Text Available Objective: The present study was aimed at evaluating the viability of replacing 18F with 99mTc in dose calibrator linearity testing. Materials and Methods: The test was performed with sources of 99mTc (62 GBq and 18F (12 GBq whose activities were measured up to values lower than 1 MBq. Ratios and deviations between experimental and theoretical 99mTc and 18F sources activities were calculated and subsequently compared. Results: Mean deviations between experimental and theoretical 99mTc and 18F sources activities were 0.56 (± 1.79% and 0.92 (± 1.19%, respectively. The mean ratio between activities indicated by the device for the 99mTc source as measured with the equipment pre-calibrated to measure 99mTc and 18F was 3.42 (± 0.06, and for the 18F source this ratio was 3.39 (± 0.05, values considered constant over the measurement time. Conclusion: The results of the linearity test using 99mTc were compatible with those obtained with the 18F source, indicating the viability of utilizing both radioisotopes in dose calibrator linearity testing. Such information in association with the high potential of radiation exposure and costs involved in 18F acquisition suggest 99mTc as the element of choice to perform dose calibrator linearity tests in centers that use 18F, without any detriment to the procedure as well as to the quality of the nuclear medicine service.

  9. A {sup 99m}Tc Generator using PZC for (n,{gamma}) {sup 99}Mo

    Energy Technology Data Exchange (ETDEWEB)

    Adang, H.G.; Mutalib, A.; Suparman, I.; Hamid; Purwadi, B.; Pancoko, M.; Setiowati, S.; Yulianti, V.; Robertus, D.H. [Radioisotope Production Center, National Atomic Energy Agency Kawasan PUSPIPTEK, Serpong (Indonesia)

    1998-10-01

    The high performance adsorbent Poly Zirconium Compound (PZC) was produced by Department of Radioisotope, Japan Atomic Energy Research Institute. This compound was developed as an adsorbent for natural Mo (n,{gamma}) {sup 99}Mo-{sup 99m}Tc Generator. In the present paper, we report the performance of the PZC for a {sup 99m}Tc Generator which was focused on the yield, on elution profile and {sup 99}Mo breakthrough. (author)

  10. Evaluation of breast MIBI-Tc 99 m scintigraphy for the detection of breast cancer

    Energy Technology Data Exchange (ETDEWEB)

    Loriaux, C.; Baeyens, L.; Paternot, J.; Martin, P.; Verhas, M. [Hospital Universitaire Brugmann, Bruxelles (Belgium). Service de Medecine Nucleaire et Clinique de Senologie

    1997-12-31

    Full text. The aim of the work was to appreciate the contribution of MIBI-Tc 99 m scintigraphy for the detection of breast cancer and to situate this procedure in comparison with mammography 5 (X-ray M) and echography (E C). 21 patients presenting suspected lesion on clinical evaluation/or mammography were referred to the department for breast MIBI-Tc 99 m scintigraphy. 10 min after IV injection of MIBI-Tc 99 m 3 planar scan were realized with a present time of 10 min. The whole investigation including X-ray and surgical procedures were performed within 15 days. The pathologic findings were: 21 malignant lesion (18 canal cancers, 3 lobular cancers), 4 benign lesions (1 fibroadenoma, 2 granuloma, 1 fibrocystic disease). Lesions were bilateral in 3 cases and there was 1 bifocal lesion. 17 tumours were palpable and 8 not palpable, 10 lesion were <1 cm (40%), 9 were between 1,1 and 2,5 cm (36%) and 7 were >2,5 cm (24%). The overall sensitivity and specificity for MIBI-Tc 99 m were 67% and 75% respectively. For the group of palpable tumours, sensitivity of MIBI-Tc 99 m was 86%. In three cases, the X-ray M was difficult to interpret, the MIBI-Tc 99 m was true positive. In 3 false positive X-ray M procedures, the scintigraphy was true negative but, whatever the size of the tumor the best sensitivity was obtained with X-ray M (71%) and E C (70%) while for MIBI Tc-99 m, sensitivity was less (67%). MIBI-Tc 99 m appears to be a complementary tool in cases of difficulty of interpretation of mammography particularly in case of recurrence of the disease

  11. Biological behavior of {sup 99m}Tc(v)DMSA in mice

    Energy Technology Data Exchange (ETDEWEB)

    Correia, Marilia B.L.; Silva, Orion M.C. da; Magnata, Simey S.S.L.P. [Universidade Federal de Pernambuco, Recife, PE (Brazil). Dept. de Energia Nuclear. Centro de Tecnologia e Geociencias]. E-mail: marilialiborio@ig.com.br; Barros, Andre L.S.; Catanho, Maria Teresa J. de A. [Universidade Federal de Pernambuco, Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia]. E-mail: mariajansem@terra.com.br; Lima, Fabiana F. de [Centro Regional de Ciencias Nucleares CRCN/NE-CNEN, Recife, PE (Brazil)]. E-mail: fflima@cnen.gov.br

    2007-07-01

    The {sup 99m}Tc(v)DMSA is a tumor-seeking agent which has been reported in bone metastasis and others tumors scintigraphies. The bone affinity and tumor uptake has been researched to elucidate its mechanism and biological behavior. The aim of this study was to obtain {sup 99m}Tc(v)DMSA from a conventional DMSA kit, to evaluate its biodistribution in an animal model, and to verify the efficiency of this method based on literature data. DMSA kit (produced by IPEN) were used to prepare {sup 99m}Tc(v)DMSA by reconstituting the lyophilized kit with 0.2mL of 3.5% NaHCO{sub 3} and addition of {sup 99m}TcO{sup 4}{sup -}. The biodistribution assays were made with mice (130, 115 and 70 days old), males and females. To each assay, the control group (n=5) received intravenous {sup 99m}Tc-DMSA and the experimental group (n=5) received {sup 99m}Tc(v)DMSA. After 30 min or 1 h, the animals were sacrificed, the organs excised and the activity measured by a gamma counter. The uptake percentage per gram (% uptake/g), tissue/blood ratio (kidney/blood - K/BL) and kidney/bone ratio (K/BO) were evaluated. In all assays there were different significant biodistribution (p{<=}0.05) between control and experimental groups, the results showed the less kidney uptake of {sup 99m}Tc(v)DMSA while increase bone affinity as young as be the animal. The {sup 99m}Tc(v)DMSA arising from DMSA kit by modified binding method was compatible with literature data. (author)

  12. Dose calibrator linearity test: {sup 99m}Tc versus {sup 18}F radioisotopes

    Energy Technology Data Exchange (ETDEWEB)

    Willegaignon, Jose; Coura-Filho, George Barberio; Garcez, Alexandre Teles, E-mail: willegaignon@hotmail.com [Instituto do Cancer do Estado de Sao Paulo Octavio Frias de Oliveira (ICESP), Sao Paulo, SP (Brazil); Sapienza, Marcelo Tatit; Buchpiguel, Carlos Alberto [Universidade de Sao Paulo (FM/USP), Sao Paulo, SP (Brazil). Fac. de Medicina; Alves, Carlos Eduardo Gonzalez Ribeiro; Cardona, Marissa Anabel Rivera; Gutterres, Ricardo Fraga [Comissao Nacional de Energia Nuclear (CNEN), Rio de Janeiro, RJ (Brazil)

    2015-01-15

    Objective: the present study was aimed at evaluating the viability of replacing {sup 18}F with {sup 99m}Tc in dose calibrator linearity testing. Materials and methods: the test was performed with sources of {sup 99m}Tc (62 GBq) and {sup 18}F (12 GBq) whose activities were measured up to values lower than 1 MBq. Ratios and deviations between experimental and theoretical {sup 99m}Tc and {sup 18}F sources activities were calculated and subsequently compared. Results: mean deviations between experimental and theoretical {sup 99m}Tc and {sup 18}F sources activities were 0.56 (± 1.79)% and 0.92 (± 1.19)%, respectively. The mean ratio between activities indicated by the device for the {sup 99m}Tc source as measured with the equipment precalibrated to measure {sup 99m}Tc and {sup 18}F was 3.42 (± 0.06), and for the {sup 18}F source this ratio was 3.39 (± 0.05), values considered constant over the measurement time. Conclusion: the results of the linearity test using {sup 99m}Tc were compatible with those obtained with the {sup 18}F source, indicating the viability of utilizing both radioisotopes in dose calibrator linearity testing. Such information in association with the high potential of radiation exposure and costs involved in {sup 18}F acquisition suggest {sup 99m}Tc as the element of choice to perform dose calibrator linearity tests in centers that use {sup 18}F, without any detriment to the procedure as well as to the quality of the nuclear medicine service. (author)

  13. Clinical evaluation of renal function using {sup 99m}Tc-DMSA scintigram

    Energy Technology Data Exchange (ETDEWEB)

    Moon, Tae Yong [College of Medicine, Pusan National University, Busan (Korea, Republic of)

    1991-09-15

    {sup 99m}Tc-DMSA (dimercaptosuccinic acid) renal uptake rates have provided an index for the cortical functioning mass and also made feasible a quantitative assessment of differential renal function by setting up a regression equation such as y=0.841 x -4.5746 (y:quantitative renal uptake rate, x:simple renal uptake rate). The authors have investigated {sup 99m}Tc-DMSA renal uptake rates in order to evaluate the renal function according to 1 group with normal kidneys and 5 groups with renal diseases and to compare with serum BUN and creatinine in 335 cases which underwent {sup 99m}Tc-DMSA renal scintigram during the last 3 years at Pusan National University Hospital. The quantitative renal uptake rates of {sup 99m}Tc-DMSA at 3 hours after intravenous injection were 50.1 {+-} 1.5% in the normal kidneys group, 49.2 {+-} 3.4% in the healthy solitary kidneys group, 28.3 {+-} 3.9% in the pathologic solitary kidneys group, 45.9 {+-} 1.3% in the unilateral nephropathy group, 26.0 {+-} 3.2% in the bilateral symmetric nephropathy group, and 32.3 {+-} 3.9% in the bilateral dissymmetric nephropathy group. When the diagnostic sensitivity of {sup 99m}Tc-DMSA renal uptake rates was compared with those of serum BUN and creatinine, the {sup 99m}Tc-DMSA renal uptake rates were more sensitive than the latter in the true positive ratio. The {sup 99m}Tc-DMSA renal scintigram provides good renal image quality concomitantly. So {sup 99m}Tc-DMSA renal scintigram could provide important clues in determining the diagnosis, therapeutic effectiveness, and prognosis of renal diseases.

  14. Evaluation of inflammatory bowel disease with /sup 99mTc-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Kadir, S.; Strauss, H.W.

    1979-02-01

    Technetium-99m-labelled DTPA was found to localize in segments of bowel with inflammation due to ulcerative colitis, regional enteritis, and other forms of enterocolitis. The concentration of tracer was apparently related to the clinical activity of the disease process. Imaging with /sup 99m/Tc-DTPA may offer an appealing, noninvasive alternative for identifying and following up patients with inflammatory bowel disease.

  15. Dose calibrator linearity test: 99mTc versus 18F radioisotopes*

    Science.gov (United States)

    Willegaignon, José; Sapienza, Marcelo Tatit; Coura-Filho, George Barberio; Garcez, Alexandre Teles; Alves, Carlos Eduardo Gonzalez Ribeiro; Cardona, Marissa Anabel Rivera; Gutterres, Ricardo Fraga; Buchpiguel, Carlos Alberto

    2015-01-01

    Objective The present study was aimed at evaluating the viability of replacing 18F with 99mTc in dose calibrator linearity testing. Materials and Methods The test was performed with sources of 99mTc (62 GBq) and 18F (12 GBq) whose activities were measured up to values lower than 1 MBq. Ratios and deviations between experimental and theoretical 99mTc and 18F sources activities were calculated and subsequently compared. Results Mean deviations between experimental and theoretical 99mTc and 18F sources activities were 0.56 (± 1.79)% and 0.92 (± 1.19)%, respectively. The mean ratio between activities indicated by the device for the 99mTc source as measured with the equipment pre-calibrated to measure 99mTc and 18F was 3.42 (± 0.06), and for the 18F source this ratio was 3.39 (± 0.05), values considered constant over the measurement time. Conclusion The results of the linearity test using 99mTc were compatible with those obtained with the 18F source, indicating the viability of utilizing both radioisotopes in dose calibrator linearity testing. Such information in association with the high potential of radiation exposure and costs involved in 18F acquisition suggest 99mTc as the element of choice to perform dose calibrator linearity tests in centers that use 18F, without any detriment to the procedure as well as to the quality of the nuclear medicine service. PMID:25798005

  16. Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m

    Institute of Scientific and Technical Information of China (English)

    ABREU P.R.C.; ALMEIDA M.C.; BERNARDO R.M.; BERNARDO L.C.; BRITO L.C.; GARCIA E.A.C.; FONSECA A.S.; BERNARDO-FILHO M.

    2006-01-01

    Psidium guajava (guava) leaf is a phytotherapic used in folk medicine to treat gastrointestinal and respiratory disturbances and is used as anti-inflammatory medicine. In nuclear medicine, blood constituents (BC) are labelled with technetium-99m (99mTc) and used to image procedures. However, data have demonstrated that synthetic or natural drugs could modify the labelling of BC with 99mTc. The aim of this work was to evaluate the effects of aqueous extract of guava leaves on the labelling of BC with 99mTc. Blood samples of Wistar rats were incubated with different concentrations of guava extract and labelled with 99mTc after the percentage of incorporated radioactivity (%ATI) in BC was determined. The results suggest that aqueous guava extract could present antioxidant action and/or alters the membrane structures involved in ion transport into cells, thus decreasing the radiolabelling of BC with 99mTc. The data showed significant (P<0.05) alteration of ATI in BC from blood incubated with guava extract.

  17. Tc-99m-EDDA-HYNIC-TOC somatostatin receptor scintigraphy in patients with carcinoids

    Directory of Open Access Journals (Sweden)

    Vlajković Marina

    2014-11-01

    Full Text Available The aim of this paper is to determine the influence of Ki-67 proliferation index on somatostatin receptor scintigraphy (SSRS with Tc-99m-EDDA-HYNIC-TOC (Tc-99m-Tektrotyd somatostatin analogue in patients with carcinoid tumors. Sixty-one patients (31 female, 30 male; age range: 33-76 years were examined: 13 patients highly suspected of having a carcinoid, and 48 patients who had undergone the surgical removal of the tumor. Whole body SSRS at 4 h postinjection, spot scintigrams and SPECT of the selected regions were obtained for all patients. Tc-99m-Tektrotyd scintigraphy was classified as true positive in 26 out of 30 and true negative in 24 out of 28 patients. The sensitivity of Tc-99mTc-Tektrotyd scintigraphy was found to be as high as 94.74% in the group of patients with low mitotic index Ki67 (20%. The likelihood of Tc-99m-Tektrotyd scan being positive when a carcinoid is present was found to be inversely proportional to the value of Ki67 proliferation index. The results showed that Tc-99m-Tektrotyd SSRS is a sensitive method for diagnosing and staging patients with well-differentiated carcinoid tumors. However, in poorly differentiated tumors with high Ki67 proliferation index, additional analyses are necessary for precise staging.

  18. Effect of mitochondrial viability and metabolism on technetium-99m-sestamibi myocardial retention

    Energy Technology Data Exchange (ETDEWEB)

    Crane, P.; Laliberte, R.; Heminway, S.; Thoolen, M.; Orlandi, C. (Du Pont Merck Pharmaceutical Co., Billerica, MA (United States). Radiopharmaceutical Div.)

    1993-01-01

    This study investigated the mechanism of myocardial retention of technetium-99m-sestamibi. [sup 99m]Tc-sestamibi was injected intravenously into guinea pigs, and the myocardium was homogenized and fractionated by differential centrifugation. More than 90% of myocardial [sup 99m]Tc-sestamibi was localized within the mitochondrial fraction. Calcium was found to release [sup 99m]Tc-sestamibi from the mitochondrial fraction, with an IC[sub 50] of 2.54[+-]0.98 mM. This effect was potentiated by NaCl, and inhibited by the mitochondrial calcium channel blocker ruthenium red. In vitro uptake of [sup 99m]Tc-sestamibi was found to increase from 10.5%[+-]3.0% to 61.2%[+-]0.2% with the addition of 10 mM succinate, indicating that respiration is involved. Since irreversible ischemia results in cellular and mitochondrial calcium 'overload,' and loss of mitochondrial metabolic function, [sup 99m]Tc-sestamibi should not be retained in necrotic or irreversibly ischemic myocardium, and could potentially act as a sensitive indicator of myocardial cell viability. (orig.).

  19. Effect of mitochondrial viability and metabolism on technetium-99m-sestamibi myocardial retention.

    Science.gov (United States)

    Crane, P; Laliberté, R; Heminway, S; Thoolen, M; Orlandi, C

    1993-01-01

    This study investigated the mechanism of myocardial retention of technetium-99m-sestamibi. 99mTc-sestamibi was injected intravenously into guinea pigs, and the myocardium was homogenized and fractionated by differential centrifugation. More than 90% of myocardial 99mTc-sestamibi was localized within the mitochondrial fraction. Calcium was found to release 99mTc-sestamibi from the mitochondrial fraction, with an IC50 of 2.54 +/- 0.98 mM. This effect was potentiated by NaCl, and inhibited by the mitochondrial calcium channel blocker ruthenium red. In vitro uptake of 99mTc-sestamibi was found to increase from 10.5% +/- 3.0% to 61.2% +/- 0.2% with the addition of 10 mM succinate, indicating that respiration is involved. Since irreversible ischemia results in cellular and mitochondrial calcium "overload" and loss of mitochondrial metabolic function, 99mTc-sestamibi should not be retained in necrotic or irreversibly ischemic myocardium, and could potentially act as a sensitive indicator of myocardial cell viability.

  20. Thymidine kinase enzyme selective imaging radiopharmaceutical. {sup 99m}Tc(CO){sub 3}-Ganciclovir

    Energy Technology Data Exchange (ETDEWEB)

    Gedik, B.; Teksoez, S.; Ichedef, C.; Kilcar, A.Y.; Medine, E.I.; Ucar, E. [Ege Univ., Bornova, Izmir (Turkey). Dept. of Nuclear Applications

    2013-03-01

    The aim of this study is to radiolabel Ganciclovir, known as having selective antiviral properties against thymidine kinase, with technetium tricarbonylcore ({sup 99m}Tc(CO){sub 3}{sup +}) and to investigate the biological behavior of this complex in vitro and in vivo. Commercially provided Ganciclovir (GCV) was radiolabeled with {sup 99m}Tc(CO){sub 3}{sup +}. Initially, optimum radiolabeling conditions were determined by analyzing factors such as temperature, pH and time. Quality control of the radiolabeled compound was performed. The radiolabeling yield was found to be 97%. The {sup 99m}Tc(CO){sub 3}-GCV complex also displayed good in vitro stability during the 24 h period. In vitro cell uptake studies showed that the {sup 99m}Tc(CO){sub 3}-GCV complex is highly uptaken in A-549, PC-3, HeLa cell lines according to the control group {sup 99m}Tc(I)-tricarbonyl core. The knowledge gained from in vivo and in vitro studies of {sup 99m}Tc(CO){sub 3}-GCV could contribute to the development of a new HSV1-tk gene imaging agent. (orig.)

  1. Acetylsalicylic acid and labeling of blood constituents with technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Fonseca, Adenilson de Souza da [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Dept. de Farmacologia e Psicobiologia; Frydman, Jacques Natan Grinapel; Rocha, Vanessa Camara da; Bernardo-Filho, Mario [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Biologia Roberto Alcantara Gomes. Dept. de Biofisica e Biometria

    2005-10-15

    Acetylsalicylic acid is the drug most used an anti-inflammatory agent and for secondary prevention of thrombotic phenomenon. Drugs can modify the labeling of blood constituents with technetium-99m (99m Tc). The aim of this work was to evaluate the effect of in vitro or in vivo assays with acetylsalicylic acid on the labeling of the blood constituents with 99m Tc. In vitro assay was performed with samples of whole blood from Wistar rats incubated with acetylsalicylic acid (1.0 mg/ml) for one hour before the 99m Tc-labeling process. For in vivo assay, Wistar rats were treated with acetylsalicylic acid (1.5 mg/kg) during one hour, and the whole blood was withdrawn for the 99m Tc-labeling process. Saline was used in control groups. Data showed that the fixation of 99m Tc to the blood constituents was not significantly (p>0.05) modified in in vitro and in vivo assays with acetylsalicylic acid, at least not when the experiments were carried out with the doses normally used in human beings. (author)

  2. Fraction of Tc99m-incorporations in the workplace activity in nuclear medicine; Anteil von Inkorporationen an der Arbeitsplatzaktivitaet fuer TC-99M in nuklearmedizinischen Einrichtungen

    Energy Technology Data Exchange (ETDEWEB)

    Schoenmuth, T. [VKTA Rossendorf (Germany); Alborzi, H. [LfULG Sachsen (Germany); Andeeff, M. [Uniklinikum Dresden (Germany); Lorenz, J. [SMUL Sachsen (Germany); Petzold, J. [Uniklinikum Leipzig (Germany)

    2009-07-01

    In the nuclear medicine, in particular in the area of the diagnostics, Tc-99m-Pharmaka are most often used. Besides, no regular monitoring occurred in praxi up to now. With use of the factors recommended, the requirement threshold (1 mSv committed dose in the calendar year) can be crossed in many areas. Within the scope of a study employees of different nuclear-medical facilities were examined in the period from March to July 2008. In the result of more than 200 measurements was founded out that a regular incorporation monitoring is not necessary for the examined employees. The estimated dose values (actual committed dose in the calendar year) was by the majority in an area below 0.1 mSv. If single evaluations of dose values are necessary an incorporation factor of 1E-07 is recommended by using connections on the basis of Tc-99m in the area of the nuclear-medical. (orig.)

  3. Efficacy of 99mTc-MDP in treatment of osteoporosis%99mTc-MDP治疗骨质疏松症疗效评价

    Institute of Scientific and Technical Information of China (English)

    郭忠; 张彩杰; 吴乃宝; 张维奇

    2009-01-01

    目的 评价稳定性核素99m锝-亚甲基二膦酸盐(99mTc-MDP)治疗骨质疏松症的效果.方法 93例骨质疏松症患者随机分为治疗组58例与对照组35例.治疗组采用99mTc-MDP注射治疗:99mTc 10 ml及MDP 10 ml加入0.9%氯化钠注射液50 ml中静脉滴注,1次/d,10~15 d为1个疗程;对照组常规口服钙剂及维生素D制剂.分别观察2组治疗前及治疗3、6、12个月时血中骨钙素(BGP)、骨特异性碱性磷酸酶(BALP)及骨密度(BMD)的变化.结果 治疗组治疗3个月后BMD、BGP值出现增高,在6个月时明显增高(P均<0.05);BALP值逐渐降低,与治疗前比较差异有统计学意义(P均<0.05);对照组治疗前后3项指标均无变化.治疗6、12个月时2组各指标的比较差异有统计学意义(P均<0.05).结论 99mTc-MDP治疗能较快地增加机体BMD,降低骨吸收,增加骨强度,对骨质疏松症有较好疗效.

  4. Part of the HIG-99mTc in the inflammatory illness of intestine. Role de la HIG-99mTc dans la maladie inflammatoire intestinale

    Energy Technology Data Exchange (ETDEWEB)

    Lancha, C.; Curto, L.M.M.; Mitjavila, M.; Vila, T. (Hopital Emaon et Cajal, Madrid (Spain)); Prat, E.; Abos, M.D.; Banzo, J.; Delgado, M. (Hopital Universitaire, Zaragaza (Spain))

    1993-04-01

    The diagnosis of intestine inflammatory illness is based on endoscopy, radiological exploration with contrast product and histological study. This kind of exploration can be contra-indicated during acute episode because of serious complications. Several methods with isotopes were applied because of their low aggressiveness. We realized a study in the aim to precise the role of (HIG)-99mTc (non specific human immunoglobulin) in the evaluation of diagnosis of the intestine inflammatory illness.

  5. Minimizing {sup 99m}Tc incorporation of the staff during lung ventilation studies; Verringerung der Inkorporation von {sup 99m}Technetium beim Personal waehrend Lungenventilationsuntersuchungen

    Energy Technology Data Exchange (ETDEWEB)

    Petzold, J.; Fundke, R.; Petzold, L.; Sabri, O.; Seese, A. [Universitaetsklinikum Leipzig (Germany). Klinik und Poliklinik fuer Nuklearmedizin; Alborzi, H. [Universitaetsklinikum Dresden (Germany)

    2007-07-01

    The causes of incorporation of {sup 99m}Tc of the staff during nuclear medical lung ventilation studies are investigated with the aim of minimizing it. It is shown that the incorporation of medical staff can be considerably reduced from more than 500 kBq per lung study to less than 500 Bq by some simple modifications of the technical equipment and small changes in the examination procedure. (orig.)

  6. Effect of methylprednisolone upon technetium-99m pyrophosphate assessment of myocardial necrosis in the canine countershock model

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, R.M.; Hayslett, J.P.; Downing, S.E.; Berger, H.J.; Donabedian, R.K.; Zaret, B.L.

    1977-12-01

    RRepeat DC countershock reproducibly results in myocardial necrosis in dogs. In this model, myocardial technetium-/sup 99m/ pyrophosphate (PYP) uptake correlates linearly with tissue creatine kinase depletion (r = -0.83). The effect of pretreatment with methylprednisolone (MP) was studied with PYP in 25 dogs. In myocardium damaged by countershock, 12 MP dogs had higher tissue radioactivity sample:normal (S:N) ratios than control (P < 0.05), suggesting increased tissue injury. However, by several other measures of tissue damage, the two groups did not differ. MP-elevated PYP S:N ratios were explained by reduced PYP activity in normal myocardium of MP dogs. Further experiments in 21 dogs revealed that renal PYP clearance, which correlated with glomerular filtration rate (GFR) as measured by creatinine clearance, was increased in MP dogs, resulting in accelerated urinary excretion of PYP (46.9 +- 3.6 vs 35.8 +- 2.4 percent injected dose in one hour, P < 0.01), and reduced blood PYP. Thus, MP does not modify countershock-induced myocardial injury. However, by increasing GFR, MP increased PYP excretion, resulting in lowered blood and normal zone myocardial PYP, thereby spuriously affecting myocardial PYP tissue uptake data.

  7. Lyophilized kits of diamino dithiol compounds for labelling with {sup 99m}-technetium. Pharmacokinetics studies and distribution compartmental models of the related complexes; Conjuntos de reativos liofilizados de compostos diaminoditiolicos para marcacao com tecnecio-99m. Estudo farmacocinetico e elaboracao de modelos compartimentalizados dos respectivos complexos

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Elaine Bortoleti de

    1995-07-01

    The present work reflects the clinical interest for labelling diamino dithiol compounds with technetium-99m. Both chosen compounds, L,L-Ethylene dicysteine (L,L-EC) and L,L-Ethylene dicysteine diethyl esther (L,L-ECD) were obtained with relative good yield and characterized by IR and NMR. The study of labelling conditions with technetium-99m showed the influence of the type and mass of reducing agent as well as the pH on the formation of complexes with desired biological characteristics. Radiochemical purity was determined by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Lyophilised kits of L,L-EC and L,L-ECD for labelling with {sup 99m}Tc were obtained, with stability superior to 120 days, when stored under refrigeration, enabling the kits marketing. The ideal formulation of the kits as well as the use of liquid nitrogen in the freezing process, determined the lyophilization success. Distribution biological studies of the {sup 99m}Tc complexes were performed on mice by invasive method and on bigger animals by scintigraphic evaluation. Biological distribution studies of the complex {sup 99m}Tc-L,L-EC showed fast blood clearance, with the elimination of about 90% of the administered dose after 60 minutes, almost exclusively by the urinary system. The biological distribution results were adjusted to a three compartmental distribution model, as expected for a radiopharmaceutical designed to renal dynamic studies, with tubular elimination. The complex interaction with renal tubular receptors is related with structural characteristics of the compound, more specifically with the presence and location of polar groups. In comparison with {sup 99m}Tc-L,L-EC, biological studies of the complex {sup 99m}Tc -L,L-ECD showed different distribution aspects, despite some structural similarities. The presence of ethyl groups confers to the complex neutrality and lipophilicity. It cross the intact blood brain barrier and is retained in the brain

  8. Magnesium-molybdenum compounds as matrices of generators of {sup 99m} Tc; Compuestos de magnesio-molibdeno como matrices de generadores de {sup 99m} Tc

    Energy Technology Data Exchange (ETDEWEB)

    Jimenez M, T.S.; Monroy G, F. [ININ, A.P. 18-1027, 11801 Mexico D.F. (Mexico)]. E-mail: thania_susana@terra.com.mx

    2004-07-01

    The generator system of radionuclides more diffused, and used in the world, it is the{sup 99}Mo / {sup 99m} Tc. These use {sup 99} Mo, product of fission of the {sup 235} U of very high specific activity, adsorbed on alumina (0.2% of {sup 99} Mo/gram of alumina). An alternative for the production of generators of low activity specifies, via the reaction {sup 98} Mo(n, {gamma}) {sup 99} Mo, it is based on the use of compounds with molybdates base, as matrices of the generators {sup 99} Mo / {sup 99m} Tc. In this work is proposed to develop a generator at base of compounds of magnesium molybdates that could be irradiated after its synthesis, given the short half life of the only radioisotope produced by magnesium: {sup 27} Mg (t{sub 1/2} = 9.46 m). In this work two parameters were studied, fundamental in the preparation of the magnesium molybdates, matrices of the generators {sup 99}Mo / {sup 99m}Tc, and their influence in the efficiency and radionuclide purity: the washing of the gels previous to its irradiation and the molar ratio Mo:Mg. The magnesium molybdates non washing presents bigger efficiencies (72%), but they don't fulfill a smaller percentage to 0.015% of {sup 99} Mo, neither with a radiochemical purity of 90%, except when the molar ratio Mo: Mg of 1:1.08 which provide the best results. (Author)

  9. Evaluation of ethinylestradiol effect on labelling red blood cells with Tc-99m; Avaliacao do efeito do etinilestradiol sobre a marcacao de hemacias com tecnecio-99m

    Energy Technology Data Exchange (ETDEWEB)

    Braga, A.C.S.; Oliveira, J.F.; Santos, J.S.; Oliveira, M.B.N.; Gutfilen, B.; Bernardo-Filho, Mario [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Biologia Roberto Alcantara Gomes. Dept. de Biofisica e Biometria

    1999-11-01

    Significant alterations on the radiopharmaceutical distribution in humans are caused by drug interactions. The labeling red blood cells with technetium-99m is a daily routine procedure in nuclear medicine. Here, we investigated if the ethinylestradiol, an oral contraceptive, could alter the labeling of RBC with Tc-99m. Samples of blood with acid citrate dextrose were incubated with ethynilestradiol. Then, different concentrations of Sn C L{sub 2} were added and, after that, Tc-99m was added. Samples were centrifuged and plasma (P) and cells (C) were separated. the results showed that the drug studied decreased the uptake of radioactivity (%ATI) in the C to the reducing agent in the concentration of 1.2 (from 92.3 to 78.0) and increased in 12.0 (18.8 to 36.0) and in 24.0 (22.8 to 32.0){mu}/ml of Sn C L{sub 2}. The obtained results can be explained by the fact that this drug could alter the membrane permeability to the transport of stannous and/or pertechnetate ions. (author) 18 refs., 2 tabs.

  10. A {sup 99m}Tc-labeled dual-domain cytokine ligand for imaging of inflammation

    Energy Technology Data Exchange (ETDEWEB)

    Liu Zhonglin, E-mail: zliu@radiology.arizona.edu [Department of Radiology, University of Arizona, Tucson, P.O. Box 245067 Tucson, AZ 85724-5067 (United States); Wyffels, Leonie; Barber, Christy [Department of Radiology, University of Arizona, Tucson, P.O. Box 245067 Tucson, AZ 85724-5067 (United States); Hui, Mizhou M. [AmProtein, Inc. San Gabriel, CA (United States); Woolfenden, James M. [Department of Radiology, University of Arizona, Tucson, P.O. Box 245067 Tucson, AZ 85724-5067 (United States)

    2011-08-15

    Introduction: Interleukin (IL)-1 and IL-18 are potent proinflammatory cytokines in inflammation-related diseases. Their actions are regulated by IL-1 receptor antagonist (IL-1ra) and IL-18 binding protein (IL-18bp). This study was designed to {sup 99m}Tc-radiolabel an IL-1ra and IL-18bp dual-domain cytokine ligand, IL-18bp-Fc-IL-1ra, for specific inflammation targeting. Methods: The {sup 99m}Tc-IL-18bp-Fc-IL-1ra was obtained by direct labeling via 2-iminothiolane reduction. Competitive binding of {sup 99m}Tc-labeled and unlabeled IL-18bp-Fc-IL-1ra to rat polymorphonuclear leukocytes was assessed in vitro. A mouse ear edema model was used to evaluate specific targeting properties of {sup 99m}Tc-IL-18bp-Fc-IL1ra in vivo. The correlation between {sup 99m}Tc-IL-18bp-Fc-IL-1ra uptake and {sup 111}In-labeled polymorphonuclear neutrophil infiltration was studied using ischemic-reperfused rat hearts. Results: Direct {sup 99m}Tc-labeling yielded a stable dual-domain cytokine radioligand with radiochemical purity greater than 95% after gel filtration. Competitive binding studies showed specific targeting of {sup 99m}Tc-IL-18bp-Fc-IL-1ra to inflammatory cells. The {sup 99m}Tc-IL-18bp-Fc-IL-1ra uptake was 1.80{+-}0.17 % injected dose per gram (%ID/g) in the inflamed ear without blocking, whereas uptake in the presence of IL-18bp-Fc-IL-1ra was 1.09{+-}0.08 %ID/g (P<.05). The amounts of IL-1{beta} and IL-18 were significantly increased in the inflamed ears compared to the vehicle controls. A significant correlation of {sup 99m}Tc-IL-18bp-Fc-IL-1ra with {sup 111}In-labeled neutrophil distribution was observed in the ischemic-reperfused hearts (P<.001). Conclusion: Targeting proinflammatory cytokines with {sup 99m}Tc-IL-18bp-Fc-IL-1ra may provide a suitable approach for specific detection of inflammatory sites.

  11. 99mTc-GSA SPECT显像在肝脏疾病中的应用%99mTc-GSA SPECT imaging in liver diseases

    Institute of Scientific and Technical Information of China (English)

    陈静; 李建生; 许戈良; 荚卫东; 马金良

    2013-01-01

    Asialogycoprotein receptor (ASGPR) is a specific receptor of mammalian hepatocytes,exclusively up-take the glycoprotein in the blood.The number of ASGPR correlates significantly with liver function,and the reduction of the number of ASGPR suggest liver dysfunction.Decreased ASGPR in patients with liver cirrhosis or portal hypertension or hepatocellar carcinoma,lead to varying degrees of liver dysfunction,which made it more susceptible to post operative complications.liver ASGPR scintigraphy with Technetium-99 m DTPA-galactosyl human serum albumin (99m Tc-GSA),combinating with single photon emission computed tomography (SPECT) technology can assess the functional reserve of remnant liver and predict incidence of postoperative complications,then assist to evaluate the use fulness for clinically surgical decisions.Current situation and progress of 99mTc-GSA SPECT imaging in hepatic surgery were reviewed in the paper.%去唾液酸糖蛋白受体(ASGPR)是哺乳动物肝细胞表面的特异性受体,能专一摄取血液中的糖蛋白.肝细胞表面ASGPR的数量与肝脏功能状态具有明显的相关性,受体数量减少提示肝功能不全.肝硬化、门脉高压症和肝癌患者的ASGPR数量减少,导致肝脏储备功能下降,极易发生术后并发症.锝标记的半乳糖基人血清白蛋白(99mTc-GSA)对该受体显像,结合单光子发射型计算机断层显像(SPECT)技术,可用于预测术后剩余肝脏的储备功能和术后并发症的发生,协助临床手术决定.本文就99mTc-GSA SPECT显像在肝脏外科应用的现状和进展进行综述.

  12. Investigating the role of 99mTc-TRODAT-1 SPECT imaging in idiopathic Parkinson's disease

    Institute of Scientific and Technical Information of China (English)

    GENG Yu; SHI Guo-hua; JIANG Yun; XU Ling-xun; HU Xing-yue; SHAO Yu-quan

    2005-01-01

    Objective: To investigate the role of 99mTc-TRODAT-1 SPECT in diagnosis and assessing severity of idiopathic Parkinson's disease (PD). Methods: Thirty-eight patients with primary, tentative diagnosis of PD and eighteen age-matched normal controls were studied with 99mTc-TRODAT-1 SPECT imaging. The regions of interests (ROIs) were drawn manually on cerebellum (CB), occipital cortex (OC) and three transverse plane slice-views of striatums, the semiquantitative BG (background)/[(OC+CB)/2] were then calculated. Results: A lower uptake of 99mTc-TRODAT-Ⅰ in striatums were displayed in thirty-six out of thirty-eight PD patients by visual inspection, compared to controls. In twenty-four PD cases with HYS (Hoehn and Yahr scale) stage I, a greater loss of DAT uptake was found in striatum and its subregions contralateral striatum to the affected limbs than in the same regions of the controls, although the striatal uptake was bilaterally reduced. Using Spearman correlation analysis showed that the reduction of the uptake ratios significantly correlated with the UPDRS in striatum and all its subregions in the PD group (P<0.05), a similar change was also found in the putamen by using the rating scale of Hoehn and Yahr (P<0.05).However, analysis of variance (ANOVA) did not show any relationship between the decreasing uptake of 99mTc-TRODAT-1 and increasing severity of PD patients, although the specific uptake of 99mTc-TRODAT-Ⅰ was continuously decreased in the striatum by visual inspection with the progress of PD from HYS stage Ⅰ to Ⅲ. Conclusion: 99mTc-TRODAT- 1 SPECT imaging may serve as a useful method for improving the correct diagnosis of PD. In assessing the role of99mTc-TRODAT- 1 SPECT in disease severity of PD, UPDRS can offer a comprehensive index, although the Hoehn and Yahr assessment may be available in part.

  13. Evaluation of {sup 99m}Tc-Pheophorbide-a use in infection imaging: A rat model

    Energy Technology Data Exchange (ETDEWEB)

    Ocakoglu, Kasim [Advanced Technologies Research and Application Center, Mersin University, TR 33343 Yenisehir, Mersin (Turkey); Bayrak, Elif; Onursal, Mehmet [Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova 35100, Izmir (Turkey); Yilmaz, Osman [Department of Animal Research Center, Dokuz Eylul University, Izmir (Turkey); Yurt Lambrecht, Fatma, E-mail: fatma.yurt.lambrecht@ege.edu.tr [Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova 35100, Izmir (Turkey); Holzwarth, Alfred R. [Max-Planck-Institut fuer Bioanorganische Chemie, Stiftstrasse 34-36, D-45470, Muelheim an der Ruhr (Germany)

    2011-09-15

    This study aims to prepare {sup 99m}Technetium Pheophorbide-a ({sup 99m}Tc-PH-A) complex and evaluate its efficiency as an infection imaging agent. First, PH-A was obtained from Spirulina maxima algae, and the product compound was confirmed using {sup 1}H NMR and MS (ESI) methods. The PH-A was then labeled with {sup 99m}Tc using the tin chloride method and its biological efficacy as a potential radiotracer for Staphylococcus aureus (S. aureus) infection was evaluated in bacterially infected and sterile inflamed rats. The radiochemical stability of the {sup 99m}Tc-PH-A in human serum was determined by thin-layer radiochromatography (TLRC). The radiochemical purity was 87{+-}3.2% and remained constant at more than 80{+-}0.1% even in serum for 120 min after radiolabeling. These experiments indicated that the ratio of {sup 99m}Tc-PH-A uptake in bacterially infected muscle, as compared to normal muscle, [target/non-target (T/NT)=5.6 at 1 h] was over four times higher than that in sterile inflamed muscle (T/NT=1.29 at 1 h). Disappearance of activity from the kidney and liver indicated that the urinary and hepatobiliary systems were the normal routes of excretion of the complex. {sup 99m}Technetium Pheophorbide prepared with high yield is able to localize well in the bacterially infected muscle of the rats and {sup 99m}Tc-PH-A may be developed as a radiopharmaceutical agent to distinguish infection from inflammation by nuclear imaging.

  14. Technetium-99m tetrofosmin parathyroid imaging in patients with primary hyperparathyroidism

    Energy Technology Data Exchange (ETDEWEB)

    Hiromatsu, Yuji; Ishibashi, Masatoshi; Nishida, Hidemi; Okuda, Seiya; Miyake, Ikuyo [Kurume Univ., Fukuoka (Japan). School of Medicine

    2000-02-01

    To confirm the clinical significance of {sup 99m}Tc-tetrofosmin imaging for the localization of hyperfunctioning parathyroid glands in patients with primary hyperparathyroidism. All patients were imaged with {sup 99m}Tc-tetrofosmin at 10 minutes and 2 hours after radiotracer injection, and with ultrasonography (US), computed tomography (CT), and magnetic resonance imaging (MRI). The parathyroid/ thyroid uptake ratio of {sup 99m}Tc-tetrofosmin (P/T uptake ratio) was calculated. Twenty patients with primary hyperparathyroidism were referred to our clinic, underwent surgical neck exploration or mediastinotomy and were diagnosed as having parathyroid adenoma. These patients were investigated for the preoperative localization by {sup 99m}Tc-tetrofosmin scintigraphy. {sup 99m}Tc-tetrofosmin imaging demonstrated focal uptake in 19 out of 20 patients with parathyroid adenoma. Two of the lesions were ectopic. US identified 17 parathyroid glands. CT and MRI initially detected 17 parathyroid glands. However, two additional parathyroid glands were localized on repeated CT and MRI in tandem with the results of the {sup 99m}Tc-tetrofosmin imaging. Thus, the sensitivity and specificity of tetrofosmin imaging were 95% (19/20) and 95% (19/20); US, 85% (17/20) and 94% (16/17); initial CT, 85% (17/20) and 94% (16/17); and initial MRI, 88% (17/20) and 94% (16/17), respectively. The P/T uptake ratio at 2 hours after tetrofosmin injection was correlated with the serum concentration of intact parathyroid hormone (PTH) (rs=0.47, p<0.05) and the resected tumor weight (rs=0.53, p<0.05). {sup 99m}Tc-tetrofosmin scintigraphy is useful for localization of parathyroid adenoma. Tetrofosmin uptake depends on the tumor weight and serum intact PTH levels. (author)

  15. The effect of drugs on the labeling of blood elements with technetium-99m.

    Science.gov (United States)

    Braga, A C; Oliveira, M B; Feliciano, G D; Reiniger, I W; Oliveira, J F; Silva, C R; Bernardo-Filho, M

    2000-07-01

    The influence of drugs on the labeling of red blood cells and plasma proteins with 99mTc has been reported. Any drug, which alters the labeling of the tracer, could be expected to modify the disposition of the radiopharmaceuticals. Red blood cells (RBC) labeled with technetium-99m (99mTc) are used for several evaluations in nuclear medicine. We have evaluated the effect of Thuya occidentalis, Peumus boldus and Nicotiana tabacum (tobacco) extracts on the labeling of RBC and plasma and cellular proteins with 99mTc. Blood was incubated with the drugs. Stannous chloride (SnCl2) solutions and 99mTc were added. Plasma (P) and blood cells (BC) were separated. The percentage of radioactivity (%ATI) bound to P and BC was determined. The %ATI on the plasma and cellular proteins was also evaluated by precipitation of P and BC samples with trichloroacetic acid (TCA) and isolation of soluble (SF) and insoluble (IF) fractions. The analysis of the results shows that there is a decrease in %ATI (from 97.64 to 75.89 percent) in BC with Thuya occidentalis extract. The labeling of RBC and plasma proteins can be decreased in presence of tobacco. This can be due either a direct or indirect effect (reactive oxygen species) of tobacco. The analysis of radioactivity in samples of P and BC isolated from samples of whole blood treated with Peumus boldus showed a rapid uptake of the radioactivity by blood cells in the presence of the Peumus boldus, whereas there was a slight decrease in the amount of 99mTc radioactivity in the TCA-insoluble fraction of plasma. This study shows that extracts of some medicinal plants can affect the radiolabeling of red blood cells with 99mTc using an in vitro technique.

  16. Role of /sup 99/mTc pertechnetate uptake in the evaluation of thyroid function

    Energy Technology Data Exchange (ETDEWEB)

    Sucupira, M.S.; Camargo, E.E.; Nickoloff, E.L.; Alderson, P.O.; Wagner, H.N. Jr.

    1983-01-01

    To investigate the usefulness of the 20 min /sup 99/mTc-pertechnetate uptake test, the records of 246 consecutive patients were reviewed. Of these, 192 patients (151 females, 41 males; 10 weeks to 78 years) had at least one year clinical follow-up or a confirmed diagnosis by biopsy or surgery and were included in our study. In these patients, the /sup 99/mTc pertechnetate uptake and hormonal values (T3 resin uptake, T4 RIA, T-index) were obtained. These results were then compared to the clinical diagnosis at the time of the uptake and one year later. All patients received an i.v. injection of 5 mCi of /sup 99/mTc pertechnetate. Imaging was performed using a pinhole collimator and a scintillation camera interfaced to a computer. Regions of interest for the thyroid and the background were used to calculate the 20 min /sup 99/mTc pertechnetate uptake as a percentage of the injected dose. /sup 99/mTc uptake and hormonal values were confirmatory in 158 patients (82.3%): 138 were euthyroid, 18 were hyperthyroid and 2 were hypothyroid. In 29 other patients (15.1%) the pertechnetate uptake provided useful additional information and helped to identify Hashimoto's thyroiditis (8 patients); thyroid suppression by exogenous iodide, steroids or T4 (7 patients); overtreated hyperthyroidism (1 patient); persistent hyperthyroidism (5 patients); different stages of Grave's disease (4 patients); and toxic nodular goiter (4 patients). The /sup 99/mTc uptake was misleading in 5 euthyroid patients (2.6%). We have found the /sup 99/mTc pertechnetate uptake a useful adjunct to measurement of hormonal levels in patients with suspected thyroid disease.

  17. Development of technetium-99m-based CNS receptor ligands: have there been any advances?

    Energy Technology Data Exchange (ETDEWEB)

    Johannsen, B. [Forschungszentrum Rossendorf e.V. (FZR), Dresden (Germany); Pietzsch, H.-J. [Forschungszentrum Rossendorf, Institut fuerr Bioanorganische und Radiopharmazeutische Chemie, Dresden (Germany)

    2002-02-01

    By virtue of its ideal nuclear physical characteristics for routine nuclear medicine diagnostics and its ready availability, technetium-99m is of outstanding interest in the development of novel radiopharmaceuticals. The potential for the development of {sup 99m}Tc-based radioligands for the study the receptor function in the central nervous system (CNS) is also well recognised despite the difficulties to be overcome. A fundamental challenge is the pharmacologically acceptable integration of the transition metal technetium, with its specific coordination chemistry, into the molecular entity of CNS receptor ligands. Conceptually, the ligand molecule can be assembled by three building blocks: a small neutral chelate unit, an organic linker that may also serve as a pharmacological modifier and a receptor-binding region derived from selective receptor antagonists. The recent introduction of novel technetium chelate units, particularly mixed-ligand complexes and low-valency organometallic compounds of technetium, provides an impetus for the further development of CNS receptor ligands. Moreover, progress in receptor pharmacology and the experience gained with positron emission tomography radiotracers have facilitated the design of numerous {sup 99m}Tc-based CNS receptor ligands. The formidable challenge of developing {sup 99m}Tc probes as single-photon emission tomography imaging agents targeting CNS receptors can be viewed with optimism given the successful development of [{sup 99m}Tc]TRODAT-1 as a {sup 99m}Tc complex for imaging dopamine transporters in the brain, although there are a number of receptor-specific imaging agents that have so far resisted all efforts to develop them. This review presents recent advances and discusses the remaining hurdles in the design of {sup 99m}Tc-based CNS receptor imaging agents. (orig.)

  18. Synthesis and evaluation of {sup 99m}Tc/{sup 99}Tc-MAG3-biotin conjugates for antibody pretargeting strategies

    Energy Technology Data Exchange (ETDEWEB)

    Gog, Frank B. van; Visser, Gerard W.M.; Gowrising, Radjish W.A.; Snow, Gordon B.; Dongen, Guus A.M.S. van E-mail: gams.vandongen@azvu.nl

    1998-10-01

    Four {sup 99m}Tc-MAG3-biotin conjugates were synthesized to determine their potential use in antibody pretargeting strategies for radioimmunoscintigraphy (RIS). To use these {sup 99m}Tc-MAG3-biotin conjugates as model compounds for {sup 186}Re-MAG3-biotin conjugates for radioimmunotherapy (RIT), nanomolar amounts of {sup 99}Tc were added as carrier to {sup 99m}Tc. The biotin derivatives used for the preparation of the conjugates - biocytin, biotin hydrazide, biotinyl-piperazine, and biotinyl-diaminosuccinic acid - differed at the site that is regarded to be susceptible to hydrolysis by biotinidase present in human plasma. All four conjugates were produced with high radiochemical purity, were stable in PBS, and demonstrated full binding capacity to streptavidin. The {sup 99m}Tc/{sup 99}Tc-MAG3-labeled biotinyl-piperazine and biotinyl-diaminosuccinic acid conjugates were stable in mouse as well as human plasma, whereas the corresponding biocytin and biotin hydrazide conjugates were rapidly degraded. The biodistribution in nude mice at 30 min after injection was similar for all conjugates, and a rapid blood clearance and high intestinal excretion were both observed. It is concluded that the metabolic routing of a conjugate containing biotin and MAG3 is dominated by these two moieties. For this reason, MAG3-biotin conjugates do not seem suited for pretargeted RIT, for which quantitative and fast renal excretion is a prerequisite to minimize radiation toxicity. However, in a pretargeted RIS approach the {sup 99m}Tc-MAG3-biotin conjugates might have potential.

  19. Evaluation of '9{sup 9}Mo presence in eluates of {sup 99}mTc used in nuclear medicine; Avaliacao da presenca de {sup 99}Mo em eluatos de {sup 99m}Tc utilizados em medicina nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Ribeiro, Bianca da Silva

    2010-07-01

    Tc-99m is used for diagnostic imaging in nuclear medicine through SPECT technique. It is obtained by the elution of {sup 99}Mo/{sup 99m}Tc generators. During the elution process {sup 99}Mo can be extracted becoming a radionuclidic impurity. One of the quality parameters of the eluate is the radionuclidic purity, MBT (molybdenum break through), defined as the ratio between {sup 99}Mo and {sup 99m}Tc activities in the eluate. The North-American and European pharmacopoeias restrict the {sup 99}Mo content, respectively, in 0.015 e 0.1% and, according to the International Atomic Energy Agency (IAEA), the activity ratio at the moment of administration of the radiopharmaceutical to the patient, should not exceed 0.015%. In Brazil, the control of such parameter is not obliged in official regulations. Thus, the objective of this work is to evaluate the occurrence of {sup 99}Mo in {sup 99m}Tc eluates. It was initially optimized a methodology to determine the activity of {sup 99}Mo in eluate samples. Efficiency curves were obtained for a NaI (Tl) 8'' x 4'' scintillation detector installed at the In Vivo Monitoring Laboratory (LABMIV-IRD). The methodology has been validated through the measurement of a {sup 99}Mo standard liquid source calibrated at the National Metrology Laboratory for Ionizing Radiation (LNMRI-IRD). The samples analyzed in this work were gently supplied by 5 Nuclear Medicine Clinics located in the city of Rio de Janeiro. The activities of {sup 99}mTc and {sup 99}Mo in those samples have been measured respectively at the clinics and at the LABMIV. By applying a standardized methodology, the ration between the activities were calculated. The results show that 147 out of 174 samples presented {sup 99}Mo activities above the minimum detectable activity of the technique. On the other hand, only 2 out of 147 samples surpassed the MBT limit suggested by the IAEA and have been detected in samples eluted from generators of 750 mCi. In one of the

  20. Tc-99m-tetrofosmin scintimammography: A prospective study in primary breast lesions

    Energy Technology Data Exchange (ETDEWEB)

    Lind, P. [LKH Klagenfurt (Austria). Dept. of Nuclear Medicine and Special Endocrinology; Gallowitsch, H.J. [LKH Klagenfurt (Austria). Dept. of Nuclear Medicine and Special Endocrinology; Kogler, D. [LKH Klagenfurt (Austria). Dept. of Radiology; Kresnik, E. [LKH Klagenfurt (Austria). Dept. of Nuclear Medicine and Special Endocrinology; Mikosch, P. [LKH Klagenfurt (Austria). Dept. of Nuclear Medicine and Special Endocrinology; Gomez, I. [LKH Klagenfurt (Austria). Dept. of Nuclear Medicine and Special Endocrinology

    1996-12-01

    The purpose of our study was to evaluate prospectively the sensitivity, specificity, PPV and NPV of scintimammography with a new catonic complex Tc-99m-tetrofosmin. Methods: Fourtyeight patients in whom mammography and/or high resolution ultrasonography (10 MHz) revealed suspicious breast lesions were studied with Tc-99m-tetrofosmin scintimammography. In thirty four of them biopsy and/or surgery was performed for histological evaluation. After intravenous injection of 555 MBq Tc-99m-tetrofosmin dynamic images over three minutes planar images in anterior and lateral projections and SPECT imaging including 3-D-reconstruction (20 min. p.i.) were performed. Scintimammography was evaluated as negative, equivocal (+), probably (++) or definitely (+++) positive. Results: Scintimammography with Tc-99m-tetrofosmin was negative in 18 patients (17 t.n.; 1 f.n.) and positive in 16 patients (10 t.p., 6 f.p.). The false negative scintimammography was observed in a patient with infiltrating ductal carcinoma pT1, the false positive result in a patient with fibrocystic disease; all of the five fibroadenomas were also `false` positive. Sensitivity of Tc-99m-tetrofosmin scintimammography in this prospective study was 91%, specificity 74%, PPV 63% and NPV 94%. Scintimammographic results in patients with suspicious breast lesion show, that Tc-99m-tetrofosmin accumulates in breast cancer as well as in fibroadenoma. However the high NPV of 94% excludes breast cancer in suspicious mammographic lesions in a very high degree and therefore reduces the need of biopsy and/or surgery in most of these patients. Conclusion: Our first results show that scintimammography with Tc-99m-tetrofosmin might play a role as further diagnostic step before surgery for women in whom mammography and/or ultrasonography show suspicious lesions. [Deutsch] Ziel unserer prospektiven Studie war es, die Sensitivitaet, Spezifitaet sowie den positiven (PVW) und negativen Voraussagewert (NVW) der Mammoszintigraphie

  1. Study of potential utility of new radiopharmaceuticals based on technetium-99m labeled derivative of glucose

    Energy Technology Data Exchange (ETDEWEB)

    Zeltchan, R., E-mail: r.zelchan@yandex.ru; Medvedeva, A.; Sinilkin, I.; Chernov, V. [Tomsk Cancer Research Institute, Tomsk, 634050 (Russian Federation); Tomsk Polytechnic University, Tomsk, 634050 (Russian Federation); Stasyuk, E.; Rogov, A.; Il’ina, E.; Larionova, L.; Skuridin, V. [Tomsk Polytechnic University, Tomsk, 634050 (Russian Federation)

    2016-08-02

    Purpose: to study the potential utility of 1-thio-D-glucose labeled with {sup 99m}Tc for cancer imaging in laboratory animals. Materials and method: the study was carried out in cell cultures of normal CHO (Chinese hamster ovary cells CHO) and malignant tissues MCF-7 (human breast adenocarcinoma MCF-7). To evaluate the uptake of {sup 99m}Tc-1-thio-D-glucose in normal and tumor tissue cells, 25 MBq of 1-thio-D-glucose labeled with {sup 99m}Tc was added to the vials with 3 million cells and incubated for 30 min at room temperature. After centrifugation of the vials with cells, the supernatant was removed. The radioactivity in vials with normal and tumor cells was then measured. In addition, the study included 40 mice of C57B1/6j lines with tumor lesion of the right femur. For neoplastic lesions, Lewis lung carcinoma model was used. Following anesthesia, mice were injected intravenously with 25 MBq of {sup 99m}Tc-1-thio-D-glucose. Planar scintigraphy was performed 15 minutes later in a matrix of 512x512 pixels for 5 min. Results: when measuring the radioactivity of normal and malignant cells after incubation with {sup 99m}Tc-1-thio-D-glucose, it was found that the radioactivity of malignant cells was higher than that of normal cells. The mean values of radioactivity levels in normal and malignant cells were 0.3 ± 0.15 MBq and 1.07 ± 0.6 MBq, respectively. All examined animals had increased accumulation of {sup 99m}Tc-1-thio-D-glucose at the tumor site. The accumulation of {sup 99m}Tc-1-thio-D-glucose in the tumor was on average twice as high as compared to the symmetric region. Conclusion: The present study demonstrated that {sup 99m}Tc-1-thio-D-glucose is a prospective radiopharmaceutical for cancer visualization. In addition, high accumulation of {sup 99m}Tc-1-thio-D-glucose in the culture of cancer cells and in tumor tissue of animals demonstrates tumor tropism of the radiopharmaceutical.

  2. Experimental study of radiopharmaceuticals based on technetium-99m labeled derivative of glucose for tumor diagnosis

    Science.gov (United States)

    Zeltchan, R.; Medvedeva, A.; Sinilkin, I.; Bragina, O.; Chernov, V.; Stasyuk, E.; Rogov, A.; Il'ina, E.; Larionova, L.; Skuridin, V.; Dergilev, A.

    2016-06-01

    Purpose: to study the potential utility of 1-thio-D-glucose labeled with 99mTc for cancer imaging in laboratory animals. Materials and method: the study was carried out in cell cultures of normal CHO (Chinese hamster ovary cells CHO) and malignant tissues MCF-7 (human breast adenocarcinoma MCF-7). To evaluate the uptake of 99mTc-1-thio-D-glucose in normal and tumor tissue cells, 25 MBq of 1-thio-D-glucose labeled with 99mTc was added to the vials with 3 million cells and incubated for 30 minutes at room temperature. After centrifugation of the vials with cells, the supernatant was removed. Radioactivity in vials with normal and tumor cells was then measured. In addition, the study included 40 mice of C57B 1/6j lines with tumor lesion of the right femur. For neoplastic lesions, Lewis lung carcinoma model was used. Following anesthesia, mice were injected intravenously with 25MBq of 99mTc-1-thio-D-glucose. Planar scintigraphy was performed 15 minutes later in a matrix of 512x512 pixels for 5 minutes. Results: when measuring the radioactivity of normal and malignant cells after incubation with 99mTc-1-thio-D- glucose, it was found that the radioactivity of malignant cells was higher than that of normal cells. The mean values of radioactivity levels in normal and malignant cells were 0.3±0.15MBq and 1.07±0.6MBq, respectively. All examined animals had increased accumulation of 99mTc-1-thio- D-glucose at the tumor site. The accumulation of 99mTc-1-thio-D-glucose in the tumor was on average twice as high as compared to the symmetric region. Conclusion: The present study demonstrated that 99mTc-1-thio-D-glucose is a prospective radiopharmaceutical for cancer visualization. In addition, high accumulation of 99mTc-1-thio-D-glucose in the culture of cancer cells and in tumor tissue of animals demonstrates tumor tropism of the radiopharmaceutical.

  3. Study of potential utility of new radiopharmaceuticals based on technetium-99m labeled derivative of glucose

    Science.gov (United States)

    Zeltchan, R.; Medvedeva, A.; Sinilkin, I.; Chernov, V.; Stasyuk, E.; Rogov, A.; Il'ina, E.; Larionova, L.; Skuridin, V.

    2016-08-01

    Purpose: to study the potential utility of 1-thio-D-glucose labeled with 99mTc for cancer imaging in laboratory animals. Materials and method: the study was carried out in cell cultures of normal CHO (Chinese hamster ovary cells CHO) and malignant tissues MCF-7 (human breast adenocarcinoma MCF-7). To evaluate the uptake of 99mTc-1-thio-D-glucose in normal and tumor tissue cells, 25 MBq of 1-thio-D-glucose labeled with 99mTc was added to the vials with 3 million cells and incubated for 30 min at room temperature. After centrifugation of the vials with cells, the supernatant was removed. The radioactivity in vials with normal and tumor cells was then measured. In addition, the study included 40 mice of C57B1/6j lines with tumor lesion of the right femur. For neoplastic lesions, Lewis lung carcinoma model was used. Following anesthesia, mice were injected intravenously with 25 MBq of 99mTc-1-thio-D-glucose. Planar scintigraphy was performed 15 minutes later in a matrix of 512x512 pixels for 5 min. Results: when measuring the radioactivity of normal and malignant cells after incubation with 99mTc-1-thio-D-glucose, it was found that the radioactivity of malignant cells was higher than that of normal cells. The mean values of radioactivity levels in normal and malignant cells were 0.3 ± 0.15 MBq and 1.07 ± 0.6 MBq, respectively. All examined animals had increased accumulation of 99mTc-1-thio-D-glucose at the tumor site. The accumulation of 99mTc-1-thio-D-glucose in the tumor was on average twice as high as compared to the symmetric region. Conclusion: The present study demonstrated that 99mTc-1-thio-D-glucose is a prospective radiopharmaceutical for cancer visualization. In addition, high accumulation of 99mTc-1-thio-D-glucose in the culture of cancer cells and in tumor tissue of animals demonstrates tumor tropism of the radiopharmaceutical.

  4. New bone-seeking agent. Animal study of Tc-99m-incadronate

    Energy Technology Data Exchange (ETDEWEB)

    Shigematsu, Makoto; Shiomi, Susumu; Iwao, Hiroshi; Ochi, Hironobu [Osaka City Univ. (Japan). Graduate School of Medicine

    2002-02-01

    Disodium cycloheptylaminomethylenediphosphonate monohydrate (incadronate disodium) is a third-generation bisphosphonate compound which potently inhibits bone resorption, and a highly effective drug in the treatment of metastatic bone disease. We first labeled incadronate disodium with {sup 99m}Tc, and examined its biodistribution and bone uptake after intravenous injection in rats to assess its potential for clinical use as a bone-seeking agent for judgment of the therapeutic effect of incadronate on bone metastases. Bone scan with {sup 99m}Tc-labeled incadronate ({sup 99m}Tc-incadronate) may yield important information prior to the use of incadronate for treatment of bone metastases. Synthesis of {sup 99m}Tc-incadronate was carried out by reduction of {sup 99m}Tc-pertechnetate in the presence of SnCl{sub 2} and N{sub 2} gas. Normal rats were injected with 18.5 MBq (0.5 mCi) {sup 99m}Tc-incadronate in a volume of 0.1 ml intravenously and then sacrificed at 15 min, 30 min, 1 h or 2 h (six rats at each time point) after injection. Samples of muscle, stomach, small intestine, kidney, liver and bone (femur) were taken and weighed. In addition, a 1-ml sample of blood was drawn from the heart, and urine was taken from the urinary bladder immediately after sacrifice. Samples were measured for radioactivity and expressed as percent uptake of injected dose per gram or per milliliter (% ID/g or ml). Bone-to-blood and bone-to-muscle uptake ratios were determined from the % ID/g or ml values for these organs. The greatest accumulation of {sup 99m}Tc-incadronate was found in bone. Radioactivity in bone was as high as 3.22{+-}0.68% ID/g at 2 hours after injection. Scintigraphic images of {sup 99m}Tc-incadronate in normal rats revealed highly selective skeletal uptake. {sup 99m}Tc-incadronate exhibited high uptake in bone, and relatively low uptake in soft tissue, suggesting that it may be useful as a bone-seeking agent for judgment of the therapeutic effect of incadronate on

  5. Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study

    Directory of Open Access Journals (Sweden)

    Priscilla Brunelli Pujatti

    2005-10-01

    Full Text Available Technetium-99m (99mTc has been the radionuclide of choice for nuclear medicine procedures and experimental research. Because of its optimal nuclear properties, 99mTc is suitable for high efficiency detection with the advantage of reduced radiological waste. Crotalus venom (CV has been shown to reduce tumors in clinical studies and tissue distribution studies are very important for clinical use. The goal of this work was to obtain CV labeled with 99mTc which preserves its biological activity. After labeling, biological activity was assessed by hemolytic activity evaluation. Labeled and crude venom caused indirect hemolysis provided that the incubation medium contained an exogenous source of lecithin. High yield radiolabeled-CV was obtained and biological activity was preserved. The results suggest that 99mTc-CV can be a useful tool for biodistribution studies.Tecnécio-99m tem sido o radioisótopo de escolha para procedimentos médicos e pesquisas experimentais. Em decorrência de suas propriedades nucleares, 99mTc é adequado para detecção de alta eficiência com a vantagem do baixo risco radiológico. O veneno de Crotalus (CV apresentou propriedades antitumorais em estudos clínicos e estudos de biodistribuição são fundamentais em pesquisas clínicas. Esse trabalho teve como objetivo obter um análogo de veneno de Crotalus marcado com 99mTc que preservasse sua atividade biológica. Após a marcação, a atividade biológica foi avaliada através do ensaio de atividade hemolítica. Veneno nativo e marcado apresentaram atividade hemolítica indireta quando incubados em um meio contendo uma fonte exógena de lecitina. Obteve-se um alto rendimento de marcação e a atividade biológica das moléculas foi preservada. Nossos resultados sugerem que 99mTc-CV pode representar uma ferramenta muito útil para estudos de biodistribuição.

  6. Clinical evaluation of technetium-99m infecton for the localisation of bacterial infection

    Energy Technology Data Exchange (ETDEWEB)

    Britton, K.E. [Department of Nuclear Medicine, St. Bartholomew`s Hospital, London (United Kingdom); Vinjamuri, S. [Department of Nuclear Medicine, St. Bartholomew`s Hospital, London (United Kingdom); Hall, A.V. [Medical Microbiology, St. Bartholomew`s Hospital, London (United Kingdom); Solanki, K. [Department of Nuclear Medicine, St. Bartholomew`s Hospital, London (United Kingdom); Siraj, Q.H. [Department of Nuclear Medicine, St. Bartholomew`s Hospital, London (United Kingdom); Bomanji, J. [Department of Nuclear Medicine, St. Bartholomew`s Hospital, London (United Kingdom); Das, S. [Medical Microbiology, St. Bartholomew`s Hospital, London (United Kingdom)

    1997-05-01

    The aim of the study was to distinguish infection from inflammation in patients with suspected infection using technetium-99m Infecton. Ninety-nine patients (102 studies) referred for infection evaluation underwent imaging with 400 MBq {sup 99m}Tc-Infecton at 1 and 4 h. Most patients had appropriate microbiological tests and about half (56) had radiolabelled white cell scans as well. No adverse effects were noted in any patient. The clinical efficacy of {sup 99m}Tc-Infecton depended in part on whether imaging was undertaken during antibiotic therapy for infection or not. In consultation with the microbiologist, 5-14 days of appropriate and successful antibiotic therapy was considered adequate to classify some results as true-negatives. The figures for sensitivity and specificity of {sup 99m}Tc-Infecton for active or unsuccessfully treated infection were 83% and 91% respectively. It is concluded that {sup 99m}Tc-Infecton imaging contributed to the differential diagnosis of inflammation. It is being used as the first imaging modality when bacterial infection is suspected. (orig.). With 2 figs., 1 tab.

  7. Solvation effects on brain uptakes of isomers of 99mTc brain imaging agents

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Analysis of electrostatic hydration free energies of the isomers of the 99mTc-BAT and 99mTc-DADT complexes is carried out using the computer simulation technique. The results show that not only a correlation exists between the logarithm of the brain uptake and the electrostatic hydration free energy for the isomers of 99mTc-brain radiopharmaceuticals, but also a linear relationship exists between the logarithm of the ratio of the brain uptake of the syn isomer to that of the anti one and the difference between the electrostatic hydration free energy of the syn-isomer and that of the anti one. Furthermore, the investigation on the important factors influencing the brain uptakes of 99mTc-radiopharmaceuticals and the reasons of the different biodistribution of the isomers of the 99mTc-complexes is explored at the molecular level. The results may provide a reference for the rational drug design of brain imaging agents.

  8. Vesicoureteral Reflux Detected with 99mTc-DTPA Renal Scintigraphy during Evaluation of Renal Function

    Directory of Open Access Journals (Sweden)

    Nevena Manevska

    2015-12-01

    Full Text Available BACKGROUND: Radionuclide techniques, as direct radionuclide cystography and 99mTc-DMSA scintigraphy, have been used in evaluation of vesicoureteral reflux (VUR and reflux nephropathy (RN in children. Dynamic 99mTc-DTPA scintigraphy is reserved for evaluation of differential renal function and obstruction in children, where hydronephrosis is detected by ultrasonography (US pre- or postnatally. CASE REPORT: Six year old boy was prenatally diagnosed with bilateral hydronephrosis. Postnatal, severe bilateral VUR was detected by voiding urethrocytography. US and 99mTc-DTPA scintigraphy performed in the first month of life showed small left kidney that participated with 2% in the global renal function. Bilateral cutaneous ureterostomy has been performed in order to obtain good renal drainage and promote optimal renal growth. Twelve months later, classic antireflux procedure was done. Control 99mTc-DTPA scintigraphy, 5 ys after antireflux surgery, revealed persisting radioactivity during the diuretic phase, in the left kidney that indicated antireflux procedure failure with VUR reappearance. CONCLUSION: 99mTc-DTPA scintigraphy is the first method of choice for long-term monitoring of individual kidney function in children with VUR and other congenital urinary tract anomalies. Additionally, it can be used as indirect radionuclide cystography when rising of radioactivity in the kidney region, during the diuretic phase can indicate presence of VUR.

  9. Vesicoureteral Reflux Detected with 99mTc-DTPA Renal Scintigraphy during Evaluation of Renal Function

    Science.gov (United States)

    Manevska, Nevena; Stojanoski, Sinisa; Majstorov, Venjamin; Pop-Gjorcheva, Daniela; Zdraveska, Nikolina; Kuzmanovska, Dafina

    2016-01-01

    BACKGROUND: Radionuclide techniques, as direct radionuclide cystography and 99mTc-DMSA scintigraphy, have been used in evaluation of vesicoureteral reflux (VUR) and reflux nephropathy (RN) in children. Dynamic 99mTc-DTPA scintigraphy is reserved for evaluation of differential renal function and obstruction in children, where hydronephrosis is detected by ultrasonography (US) pre- or postnatally. CASE REPORT: Six year old boy was prenatally diagnosed with bilateral hydronephrosis. Postnatal, severe bilateral VUR was detected by voiding urethrocytography. US and 99mTc-DTPA scintigraphy performed in the first month of life showed small left kidney that participated with 2% in the global renal function. Bilateral cutaneous ureterostomy has been performed in order to obtain good renal drainage and promote optimal renal growth. Twelve months later, classic antireflux procedure was done. Control 99mTc-DTPA scintigraphy, 5 ys after antireflux surgery, revealed persisting radioactivity during the diuretic phase, in the left kidney that indicated antireflux procedure failure with VUR reappearance. CONCLUSION: 99mTc-DTPA scintigraphy is the first method of choice for long-term monitoring of individual kidney function in children with VUR and other congenital urinary tract anomalies. Additionally, it can be used as indirect radionuclide cystography when rising of radioactivity in the kidney region, during the diuretic phase can indicate presence of VUR. PMID:27275347

  10. Tc-99m macro aggregated albumin scintigraphy – indications other than pulmonary embolism: A pictorial essay

    Science.gov (United States)

    Gandhi, Sunny J; Babu, Sanjay; Subramanyam, Padma; Shanmuga Sundaram, Palaniswamy

    2013-01-01

    Introduction: Tc-99m macro aggregated albumin (MAA) is synonymous for lung perfusion scintigraphy and is part of the study in the evaluation of pulmonary thromboembolism. We wanted to highlight the utilities of Tc-99m MAA other than pulmonary embolism as a pictorial assay. Materials and Methods: Patients referred for Tc-99m MAA scintigraphy under various indications were included in this pictorial essay. Commercially available TechneScan LyoMAA cold kit from Mallinckrodt Medical B.V., Holland was used. Acquisition protocols for different indications are described in this article. Different clinical indications (e.g., pulmonary artery stenosis, hepatopulmonary syndrome, FEV1 calculation in lung surgery planning, selective internal radiation therapy planning, venography for deep venous thrombosis, left to right cardiac shunts, etc.) where Tc-99m MAA scintigraphy was asked for; how it helped in different clinical scenarios and how it can be used clinically is explained with unique and interesting case examples and images. We also reviewed the literature to look for certain remote indications of MAA imaging for the sake of completion like – (shunt scintigraphy, peritoneopleural communication, etc.) Conclusion: Tc-99m MAA is a very useful radiopharmaceutical, which can be used for many other indications apart from the commonly used indication of lung perfusion scan in pulmonary embolism. It can provide useful clinical information in other indications, which we try to highlight in this article. PMID:24250023

  11. Role of technetium-99m pertechnetate scintigraphy in the management of extra-abdominal fibromatosis

    Energy Technology Data Exchange (ETDEWEB)

    Terui, Shoji [Div. of Nuclear Medicine, National Cancer Hospital, Tokyo (Japan); Terauchi, Takashi [Div. of Nuclear Medicine, National Cancer Hospital, Tokyo (Japan); Abe, Hiroyuki [Div. of Nuclear Medicine, National Cancer Hospital, Tokyo (Japan); Muramatsu, Yukio [Div. of Diagnostic Radiology, National Cancer Hospital, Tokyo (Japan); Fukuma, Hisatoshi [Div. of Orthopedics, National Cancer Center Hospital, Tokyo (Japan); Beppu, Yasuo [Div. of Orthopedics, National Cancer Center Hospital, Tokyo (Japan); Yokoyama, Ryohei [Div. of Orthopedics, National Cancer Center Hospital, Tokyo (Japan)

    1995-07-01

    The purpose of this study was to investigate technetium-99m pertechnetate (Tc-99m) as a tumorscanning agent in patients with extra-abdominal fibromatosis, and to establish the sensitivity of this type of scintigraphy. Eleven patients with extra-abdominal fibromatosis were studied: all but one having postsurgical recurrences. Of the 11 patients, diagnosed histologically, 5 underwent repeated Tc-99m scintigraphic follow-up examinations. The injected 370 MBq Tc-99m gave us an early scintigram within 10 min and a delayed one 2 h later. For adequate comparison, the region of interest (ROI) of the scintigram was placed over the tumor. The tumor-to-background (T/BG) count ratio was computed. Extra-abdominal fibromatoses, even recurrences, were demonstrated scintigraphically in both the early and the delayed phase, in all 11 patients. The average T/BG ratio was 2.11 in the early scintigram and 2.15 in the delayed one. The sensitivity and the specificity were both 100%. Tc-99m scintigraphy has proved useful in detecting extra-abdominal fibromatoses and in the follow-up of patients. (orig.)

  12. Efficacy of (99m)Tc-EDDA/HYNIC-TOC SPECT/CT scintigraphy in Graves' ophthalmopathy.

    Science.gov (United States)

    Zhao, Rong; Wang, Jiang; Deng, Jinglan; Yang, Weidong; Wang, Jing

    2012-01-01

    The aim of this study was to investigate the predictive role of the orbital somatostatin receptor scintigraphy with (99m)Tc-EDDA/HYNIC-TOC ((99m)Tc-TOC) to detect clinical stage of Graves' ophthalmopathy and the response to corticosteroid therapy. The subjects of the experiment were 46 patients with Graves' ophthalmopathy (GO) and four volunteers without eye disease or GO as the normal group (NG). Single photon emission computed tomography (SPECT), computed tomography (CT) and the left and right lateral position planar imaging of the heads of the all subjects were obtained 4 h after the intravenous injection of 555 MBq of (99m)Tc-TOC. The (99m)Tc-TOC SPECT/CT was repeated 3 months later. 35 (35/46) patients were received corticosteroid therapy (prednisolone, 10 mg po tid ) for 3 months, however, the other 11 patients as control groups did not receive any treatment. The treatment effect was evaluated both by the orbital (99m)Tc-TOC uptake and NOSPECS. A significant decrease in the O/OC ratio was observed in 22 GO patients between pre- and post-treatment (1.64 ± 0.13 vs. 1.21 ± 0.09, P TOC scintigraphy is a feasible technique to estimate the Graves' ophthalmopathy activity and predict the response to subsequent corticosteroid therapy in GO patients. The technique could be a useful tool for physicians not familiar with CAS determination.

  13. Radiolabelling of human platelets with sup 99m Tc-HMPAO

    Energy Technology Data Exchange (ETDEWEB)

    Vorne, M.; Honkanen, T.; Karppinen, K.; Roening, M.; Sakki, S. (Paeijaet-Haeme Central Hospital, Lahti (Finland))

    1989-01-01

    The optimum conditions for labelling platelets with lipophilic {sup 99m}Tc-hexamethyl propylene amine oxime ({sup 99m}Tc-HMPAO) were evaluated. An aseptic closed system was used throughout the procedure in patient studies. 48 ml blood were withdrawn into 12 ml ACD using sterile 60 ml plastic syringes. After mixing, the blood was transferred to sterile 10 ml vacuum tubes and platelets were isolated according to standard centrifugation procedures. The labelling efficiency was not dependent upon incubation temperature (22 deg. C, 37 deg. C) but was greater in saline than in the presence of plasma. The labelling efficiency increased with time up to 60 min in saline. The elution of {sup 99m}Tc from platelets was about 25% in plasma milieu in vitro but did not increase with time during 160 min. 5 patients with verified fresh deep vein thrombosis in the lower leg were imaged after injection of labelled autologous platelets. All 4 of the patients without anticoagulant therapy showed positive uptake of {sup 99m}Tc-HMPAO-labelled platelets, but the 5th patient - under heparin therapy - was negative in scientigraphy. Our results are encouraging and {sup 99m}Tc-HMPAO-labelled platelets offer a promising tool for evaluating various clinical situations. (author).

  14. Effect of iron deficiency anemia on the biodistribution of {sup 99m}Tc radiopharmaceuticals

    Energy Technology Data Exchange (ETDEWEB)

    Calmanovici, Gabriela P. [Radioisotopes Laboratory, Physics Department, School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 - 1113, Buenos Aires (Argentina); Salgueiro, Maria J. [Radioisotopes Laboratory, Physics Department, School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 - 1113, Buenos Aires (Argentina); Janjetic, Mariana A. [Physics Department, School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 - 1113, Buenos Aires (Argentina); Leonardi, Natalia M. [Radioisotopes Laboratory, Physics Department, School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 - 1113, Buenos Aires (Argentina); Boccio, Jose R. [Physics Department, School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 - 1113, Buenos Aires (Argentina); Zubillaga, Marcela B. [Radioisotopes Laboratory, Physics Department, School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 - 1113, Buenos Aires (Argentina)]. E-mail: mzubi@ffyb.uba.ar

    2006-05-15

    The distribution of colloids and labeled cells in organs is influenced by their intrinsic properties and by the state of the investigated subject. Iron deficiency remains an unsolved nutritional problem all over the world; one of its severe consequences is anemia. Because iron metabolism principally takes place in the liver, spleen, bone marrow, skeletal muscle and blood, we studied the effect of iron deficiency anemia on the biodistribution of {sup 99m}Tc phytate, {sup 99m}Tc gelatin colloid and {sup 99m}Tc RBC (red blood cells labeled with {sup 99m}Tc). Our results show that iron deficiency anemia modifies the pattern of biodistribution of the two colloids assayed. However, this behavior is different for both of them. This work contributes to studies that kinetically and statistically establish that iron deficiency anemia induces a significant inversion in the spleen-liver activity relationship when centellographic studies are performed with colloids such as {sup 99m}Tc phytate.

  15. Preparation and stability of the {sup 99m} Tc-HNE{sub 2} radiopharmaceutical; Preparacion y estabilidad del radiofarmaco {sup 99m} TC-HNE{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Estrada T, J

    2002-07-01

    A radiopharmaceutical is all substance containing a radioactive atom inside of its structure and what because of its pharmaceutical form, quantity and quality of radiation can be administered in the human beings with diagnostic or therapeutic aims. With the purpose to developing effective radiopharmaceuticals it is necessary to pick carefully the appropriate radionuclide in combination with the In vivo localization and the pharmacon kinetic properties of the carrier molecule. The peptides are designed by the nature to stimulate, regulate or inhibit numerous life functions, they act mainly as information transmitters and activity coordinators of several tissues in the body; it has been found that such substances are present in cells and in the body fluids in quantities extremely small, therefore the peptides have been considered as ideal agents for therapeutic applications. Elastase of human neutrophylls is a 29 kDa protease which is produced in high levels inside the neutrophyll and it is released as response for an inflammatory stimulus in infection/inflammation places. Once it liberated is quickly inhibited by the anti elastase {alpha} tripsine (HNE-2) peptide. Therefore, the neutrophylls elastase is considered as a target to obtain In vivo images of inflammatory/infectious processes by the intravenous application of {sup 99m} Tc-HNE-2. The objective of this work was to develop a labelling method with {sup 99m} Tc for the inhibitor peptide of the human neutrophyll elastase (HNE-2). Likewise, for evaluating its In vivo and In vitro stabilities. The methodology which was followed as first step to conjugate the (HNE-2) peptide with the bi chelating agents HYNIC and DTPA capable to chelate the {sup 99m} Tc metal. Therefore the attachment reactions to the peptide were realized starting from the NHS and HYNIC and the DTPA anhydride in buffer of 0.1 M, pH= 9.0/DMF (10:1) bicarbonates with a molar relation peptide/bi chelating agent 1:5. For the purification of the

  16. Preparation and stability of the {sup 99m} Tc-HNE{sub 2} radiopharmaceutical; Preparacion y estabilidad del radiofarmaco {sup 99m} TC-HNE{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Estrada T, J

    2002-07-01

    A radiopharmaceutical is all substance containing a radioactive atom inside of its structure and what because of its pharmaceutical form, quantity and quality of radiation can be administered in the human beings with diagnostic or therapeutic aims. With the purpose to developing effective radiopharmaceuticals it is necessary to pick carefully the appropriate radionuclide in combination with the In vivo localization and the pharmacon kinetic properties of the carrier molecule. The peptides are designed by the nature to stimulate, regulate or inhibit numerous life functions, they act mainly as information transmitters and activity coordinators of several tissues in the body; it has been found that such substances are present in cells and in the body fluids in quantities extremely small, therefore the peptides have been considered as ideal agents for therapeutic applications. Elastase of human neutrophylls is a 29 kDa protease which is produced in high levels inside the neutrophyll and it is released as response for an inflammatory stimulus in infection/inflammation places. Once it liberated is quickly inhibited by the anti elastase {alpha} tripsine (HNE-2) peptide. Therefore, the neutrophylls elastase is considered as a target to obtain In vivo images of inflammatory/infectious processes by the intravenous application of {sup 99m} Tc-HNE-2. The objective of this work was to develop a labelling method with {sup 99m} Tc for the inhibitor peptide of the human neutrophyll elastase (HNE-2). Likewise, for evaluating its In vivo and In vitro stabilities. The methodology which was followed as first step to conjugate the (HNE-2) peptide with the bi chelating agents HYNIC and DTPA capable to chelate the {sup 99m} Tc metal. Therefore the attachment reactions to the peptide were realized starting from the NHS and HYNIC and the DTPA anhydride in buffer of 0.1 M, pH= 9.0/DMF (10:1) bicarbonates with a molar relation peptide/bi chelating agent 1:5. For the purification of the

  17. Diagnostic performance of an expert system for interpretation of 99mTc MAG3 scans in suspected renal obstruction.

    Science.gov (United States)

    Taylor, Andrew; Garcia, Ernest V; Binongo, Jose Nilo G; Manatunga, Amita; Halkar, Raghuveer; Folks, Russell D; Dubovsky, Eva

    2008-02-01

    The purpose of the study was to compare diuresis renography scan interpretation generated by a renal expert system with the consensus interpretation of 3 expert readers. The expert system was evaluated in 95 randomly selected furosemide-augmented patient studies (185 kidneys) obtained for suspected obstruction; there were 55 males and 40 females with a mean age +/- SD of 58.6 +/- 16.5 y. Each subject had a baseline (99m)Tc-mercaptoacetyltriglycine ((99m)Tc-MAG3) scan followed by furosemide administration and a separate 20-min acquisition. Quantitative parameters were automatically extracted from baseline and furosemide acquisitions and forwarded to the expert system for analysis. Three experts, unaware of clinical information, independently graded each kidney as obstructed/probably obstructed, equivocal, and probably nonobstructed/nonobstructed; experts resolved differences by a consensus reading. These 3 expert categories were compared with the obstructed, equivocal, and nonobstructed interpretations provided by the expert system. Agreement was assessed using weighted kappa, and the predictive accuracy of the expert system compared with expert readers was assessed by the area under receiver-operating-characteristic (ROC curve) curves. The expert system agreed with the consensus reading in 84% (101/120) of nonobstructed kidneys, in 92% (33/36) of obstructed kidneys, and in 45% (13/29) of equivocal kidneys. The weighted kappa between the expert system and the consensus reading was 0.72 and was comparable with the weighted kappa between experts. There was no significant difference in the areas under the ROC curves when the expert system was compared with each expert using the other 2 experts as the gold standard. The renal expert system showed good agreement with the expert interpretation and could be a useful educational and decision support tool to assist physicians in the diagnosis of renal obstruction. To better mirror the clinical setting, algorithms to

  18. Evaluation of renal allograft dysfunction employing dynamic SPECT with {sup 99m}Tc-MAG3 and graph plot analysis

    Energy Technology Data Exchange (ETDEWEB)

    Akahira, Hideaki [Oyokyo Kidney Research Inst., Hirosaki, Aomori (Japan). Hirosaki Hospital

    1996-11-01

    To estimate renal blood flow and tubular function in transplanted kidneys, we applied the 4 compartments model and the graphic analysis method to {sup 99m}Tc-MAG3 dynamic SPECT and calculated some parameters, i.e. K1 (renal influx rate constant), K3 (tubular transporting rate constant), Vd12 (intrarenal distribution volume), and others. Twenty-three renal transplant recipients were examined and divided into following 3 groups according to their serum creatinine levels (SCr); Group I: less than 13 mg/dl (1.1{+-}0.3, n=7), Group II: 1.4-2.5 mg/dl (1.8{+-}0.3, n=11), and Group III more than 2.6 mg/dl (3.9{+-}0.9, n=5). The K3 value became lower in the order of Group I>II>III, and well correlated with blood urea nitrogen (BUN, r=-0.95, p<0.001) and creatinine clearance (Ccr, r=0.78, p<0.001). The K1 value reduced markedly in Group III despite of no difference between Group I and II. Although the K1 value also correlated with SCr, BUN and Ccr, correlation coefficients were smaller than those with the K3. Effective renal plasma flow derived from K1 and K3 showed a good correlation with the tubular extraction rate by Bubeck`s method. From these results and clinical conditions including histopathological findings, it is suggested that K1, K3 and Vd12 are useful parameters of renal central arterial blood flow, renal peripheral arteriolar blood flow and renal {sup 99m}Tc-MAG3 uptake function, respectively. (author)

  19. MO-F-CAMPUS-I-05: Radiation Dosimetry of 99mTc-IDA-D-[c(RGDfK)]2, a SPECT Agent for Angiogenesis Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Kim, J [Korea Research Institute of Standards and Science, Daejeon (Korea, Republic of)

    2015-06-15

    Purpose: Tc-99m labeled IDA-D-[c(RGDfK){sub 2} ( {sup 99m}Tc-RGD) is a recently developed radiotracer for gamma camera or single photon emission computed tomography (SPECT) imaging and promising agent for the visualization of angiogenesis. In this study, we investigated the internal radiation dosimetry of {sup 99m}Tc-RGD in humans. Methods: Six normal controls (F:M=4:2; 68.3±3.2 years; 56.5±10.7 kg) were participated in this study. Simultaneous anterior and posterior scans of whole-body were performed using dual head gamma camera system. Before the emission scan, transmission scan was performed just before injection of {sup 99m}Tc-RGD using Co-57 flood source. After an intravenous injection of 388.7±29.3 MBq of {sup 99m}Tc-RGD, six serial emission scans were performed at 0, 1, 2, 4, 8 and 24 hours post-injection. The anterior and posterior images were geometrically averaged and attenuation correction was applied using transmission scan image. Regions of interest (ROIs) were drawn on liver, gallbladder, kidneys, urinary bladder, spleen, brain, and large intestine. Time activity curves were obtained from serial emission scan and ROIs. The number of disintegrations per unit activity administered (residence time) were calculated from the area under the curve of time activity curves and injected dose of each patient. Finally, the radiation dose for each organ and effective doses were obtained using OLINDA/EXM 1.1 software and residence time. Results: High radiation doses were reported on renal and biliary excretion tracks such as urinary bladder wall, upper large intestine, kidneys, liver and gallbladder wall and their doses were 19.15±6.84, 19.28±4.78, 15.67±0.90, 9.13±1.71 and 9.09±2.03 µGy/MBq, respectively. The effective dose and effective dose equivalent were 5.08±0.53 and 7.11±0.58 µSv/MBq, respectively. Conclusion: We evaluated the radiation dose of 99mTc-RGD, which has an acceptable effective radiation dose compare to the other Tc-99m labeled radio-tracers.

  20. Synthesis and evaluation of a {sup 99m}Tc-MAMA-propyl-thymidine complex as a potential probe for in vivo visualization of tumor cell proliferation with SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Celen, Sofie [Laboratory for Radiopharmacy, K.U. Leuven, B-3000 Leuven (Belgium); Groot, Tjibbe de [Radiopharmacy, U.Z. Gasthuisberg K.U. Leuven, B-3000 Leuven (Belgium); Balzarini, Jan [Rega Institute for Medical Research, K.U. Leuven, B-3000 Leuven (Belgium); Vunckx, Kathleen [Nuclear Medicine, U.Z. Gasthuisberg K.U. Leuven, B-3000 Leuven (Belgium); Terwinghe, Christelle [Radiopharmacy, U.Z. Gasthuisberg K.U. Leuven, B-3000 Leuven (Belgium); Vermaelen, Peter [Nuclear Medicine, U.Z. Gasthuisberg K.U. Leuven, B-3000 Leuven (Belgium); Van Berckelaer, Lizette [Rega Institute for Medical Research, K.U. Leuven, B-3000 Leuven (Belgium); Vanbilloen, Hubert [Radiopharmacy, U.Z. Gasthuisberg K.U. Leuven, B-3000 Leuven (Belgium); Nuyts, Johan [Nuclear Medicine, U.Z. Gasthuisberg K.U. Leuven, B-3000 Leuven (Belgium); Mortelmans, Luc [Nuclear Medicine, U.Z. Gasthuisberg K.U. Leuven, B-3000 Leuven (Belgium); Verbruggen, Alfons [Laboratory for Radiopharmacy, K.U. Leuven, B-3000 Leuven (Belgium); Bormans, Guy [Laboratory for Radiopharmacy, K.U. Leuven, B-3000 Leuven (Belgium)]. E-mail: guy.bormans@pharm.kuleuven.be

    2007-04-15

    Introduction: Cytosolic thymidine kinase (TK1) catalyzes phosphorylation of thymidine to its monophosphate. TK1 activity is closely related with DNA synthesis, and thymidine analogs derivatized with bulky carboranylalkyl groups at the N-3 position were reported to be good substrates for TK1. Accordingly, we have synthesized {sup 99m}Tc-MAMA-propyl-thymidine and evaluated it as a potential tumor tracer. Methods: The bis(S-trityl)-protected MAMA-propyl-thymidine precursor (3-N-[S-trityl-2-mercaptoethyl]-N-[N'-(S-trityl-2-mercaptoethyl) amidoacetyl] -aminopropyl-thymidine) was prepared in three steps, and its structure was confirmed with {sup 1}H NMR and mass spectrometry. Deprotection of the thiols and labeling with {sup 99m}Tc were done in a two-step, one-pot procedure, yielding {sup 99m}Tc-MAMA-propyl-thymidine, which was analyzed with high-performance liquid chromatography, radio-LC-MS analysis (ESI+) and electrophoresis, and its log P was determined. The biodistribution in normal mice was evaluated, and its biodistribution in a radiation-induced fibrosarcoma (RIF) tumor mouse was compared with that of 3'-deoxy-3'-[{sup 18}F] fluorothymidine [{sup 18}F]FLT. Results: {sup 99m}Tc-MAMA-propyl-thymidine was obtained with a radiochemical yield of 70%. Electrophoresis indicated that the complex is uncharged, and its log P was 1.0. The molecular ion mass of the Tc complex was 589 Da, which is compatible with the hypothesized N{sub 2}S{sub 2}-oxotechnetium structure. Tissue distribution showed fast clearance from plasma primarily by the hepatobiliary pathway. Whole-body planar imaging after injection of {sup 99m}Tc-MAMA-propyl-thymidine in an RIF tumor-bearing mouse showed high uptake in the liver and the intestines. No uptake was observed in the tumor, in contrast to the clear uptake observed for [{sup 18}F] FLT visualized with {mu}PET. Conclusions: Although it has been reported that TK1 accepts large substituents at the N-3 position of the thymine ring

  1. Magnesium-molybdenum compounds as matrixes of {sup 99m} Tc generators; Compuestos de magnesio-molibdeno como matrices de generadores de {sup 99m} Tc

    Energy Technology Data Exchange (ETDEWEB)

    Jimenez M, T.S

    2005-07-01

    In order to finding new production alternatives of {sup 99}Mo/ {sup 99m}Tc generators, easy to produce and with high elutriation efficiencies, it is proposed in this work to develop a generator with basis of magnesium and molybdenum that could be irradiated after their synthesis, given the short half life of the only radioisotope produced by the magnesium: {sup 27} Mg (t{sub 1/2} = 9.46 m). It is necessary to mention that have not been reported works in relation to this type of compound, being then important to carry out basic studies on the formation and behavior of these in relation to their matrix functions of those generated of {sup 99} Mo/ {sup 99m} Tc. In this work it was determined the effect that has, the magnesium salt used in the synthesis of those molybdenum-magnesium compounds, the molar ratio Mo: Mg, the concentration of the magnesium salt, the pH of the used ammonium molybdates in the synthesis of the final compounds and the washing of the molybdates of synthesized magnesium, in the performance of the {sup 99} Mo/ {sup 99m} Tc generators. Parameters like the elutriation efficiency, the radionuclide purity, radiochemical and chemical of the eluates and their pH, were determined in each case, also its were characterized the synthesized compounds using: neutron activation analysis (NAA), X-ray diffraction (XRD) and scanning electron microscopy of high vacuum (SEM), besides of complementary techniques: infrared spectroscopy (IR), and thermal gravimetric analysis (TGA). (Author)

  2. Scintigraphic study of palpable breast nodes with {sup 99m} Tc-tetrofosmin; Estudo cintilografico de nodulos palpaveis da mama com {sup 99m} Tc - tetrofosmin

    Energy Technology Data Exchange (ETDEWEB)

    Pellini, Marcos Pinto; Fonseca, Lea Mirian Barbosa da [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina. Dept. de Radiologia; Amarante Junior, Jose Luiz de Medeiros [Hospital Naval Marcilio Dias, Rio de Janeiro, RJ (Brazil). Servico de Medicina Nuclear; Fabricio, Maria Ines Menescal [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Ginecologia

    1998-12-01

    In Brazil, breast carcinoma has the greater incidence among women. In 1996, about 31,210 new cases were diagnosed. The purpose of this study is to determine if {sup 99m} technetium-tetrofosmin preferentially concentrates within malignant palpable breast nodes, distinguishing them from the benign lumps. Fifth-four consecutive and unsolicited women, mean age 47.5 +- 10.7 years, with palpable nodes in breast, underwent breast scintigraphy with {sup 99m} technetium-tetrofosmin and excisional biopsy or fine needle puncture within interval eight weeks. Histopathologic studies demonstrated 13 cysts, 1 cyst with local inflammation, 11 fibroadenoma, 2 fibrodysplasias, 3 fibroadenomas with high cellularity, 1 fibrocystic change with local inflammation, 23 invasive intraductal carcinomas and 1 invasive lobular carcinoma. The nodes ranged from 10 to 100 mm in greater diameter. The scintigraphic study yielded 30 true-negative cases, 24 true-positives, no false-negative and 1 false-positive. This case was a fibrocystic lump with local inflammation. Two patients had metastases in axillary lymph nodes, well detected in scintigraphy. The statistic analysis showed: sensitivity, 100%; specificity, 96.8%; accuracy, 98.2%; negative predictive value, 100%; positive predictive value, 96.0%. Nonparametric tests of Fisher and Chi-square rejected the antithesis at a confidence interval of 99.9% (p < 0.001). We concluded that {sup 99m} technetium-tetrofosmin preferentially concentrates in malignant palpable breast nodes and can help to distinguish them from the benign tumors with with very high accuracy. (author)

  3. Diffuse Myocardial Uptake of {sup 99m}Tc-HDP in Multiple Myeloma

    Energy Technology Data Exchange (ETDEWEB)

    Demirel, Koray; Sadic, Murat; Korkmaz, Meliha; Comak, Aylin; Atilgan, Hasan Ikbal; Koca, Goekhan [Ministry of Health Ankara Training and Research Hospital, Ankara (Turkmenistan)

    2013-09-15

    Soft tissue uptake is a rare finding in bone scintigraphy, with an incidence of 2%. Although the mechanism has not yet been fully clarified, several causes have been reported for this unusual uptake pattern. This paper presents a case of diffuse myocardial accumulation of technetium-99m hydroxymethylene diphosphonate ({sup 99m}Tc-HDP) without either solid/visceral organ or soft tissue with multiple myeloma (MM) in skeletal scintigraphy. A 93-year-old man with hypertension and chronic heart failure for 14 years underwent bone scanning due to a 2-month history of back pain within a 1-year period of MM. Three hours later, {sup 99m}Tc-HDP late static images showed diffuse myocardial radiotracer accumulation and there were no other sites of abnormal soft tissue or visceral uptake. Myocardial accumulation had disappeared on 24-h delayed static images. This accumulation was thought to be related with AL-type amyloidosis associated with MM.

  4. Subcutaneous injection of sup 99m Tc pertechnetate at acupuncture points K-3 and B-60

    Energy Technology Data Exchange (ETDEWEB)

    Wu Chung-Chieng; Jong Shiang-Bin; Lin Chun-Ching; Chen Min-Fen; Chen Jong-Rern (Kaohsiung Medical Coll., Taiwan (China)); Chung Chieng

    1990-06-01

    The acupuncture points are known to be morphologically related to the nerves and vessels. Yet the physiological role of blood vessels in the formation of acupuncture points remains unknown. With subcutaneous injection of {sup 99m}Tc pertechnetate at the acupuncture points K-3 and B-60 and with intra-acupuncture point injection of {sup 99m}Tc pertechnetate at K-3 and B-60, a lower-limb venography like what was obtained by intravenous injection of {sup 99m}Tc macroaggregated albumin was demonstrated in the present study. It seems that some acupuncture points do play a role in drainage of tissue fluid from soft tissue into the veins. (author).

  5. 99mTc-HMPAO perfusion SPECT/CT in the diagnosis of brain death.

    Science.gov (United States)

    Derlin, Thorsten; Weiberg, Desiree

    2016-01-01

    This report describes a case of brain death (BD) evaluated by 99mTc-hexamethylpropylene amine oxime (HMPAO) single photon emission tomography/computed tomography (SPECT/CT). A 16-year-old boy with a history of rapid unexpected brain herniation due to pilocytic astrocytoma underwent 99mTc-HMPAO SPECT/CT for evaluation of brain death in the context of organ donation. Flow images demonstrated lack of blood flow to the brain, and delayed images showed absence of demonstrable radionuclide activity within the brain. SPECT/CT confirmed absence of tracer accumulation, and was deemed helpful for evaluation of the brain stem. 99mTc-HMPAO SPECT/CT is a valuable tool enabling imaging-based confirmation of BD.

  6. Present status of {sup 99}Mo-{sup 99m}Tc generator in Japan

    Energy Technology Data Exchange (ETDEWEB)

    Genka, Tsuguo [Japan Atomic Industrial Forum, Inc., Asia Cooperation Center, Tokyo (Japan)

    2003-03-01

    In Japan most of the demand of {sup 99m}Tc is relying on fission produced {sup 99}Mo imported from overseas. The (n, {gamma}) {sup 99}Mo-{sup 99m}Tc generator is not in the state of practical use but research and development are going on. Since 1995, Japan Atomic Energy Research Institute (JAERI) and Kaken Co. Ltd. have been collaborating to develop the Mo adsorbent material of poly zirconium compound (PZC) fit for so called (n, {gamma}) method. In 2000 the Kaken and the National Nuclear Energy Agency (BATAN) of Indonesia has facilitated to promote the technology of PZC based {sup 99m}Tc generator under the joint research agreement. Along with the cooperative experiments between two bodies, very promising results are coming out. Some of these results will be disclosed in the present workshop. (author)

  7. Comparative uptake of Tc-99m sestamibi and Tc-99m tetrofosmin in cancer cells and tissue expressing P-Glycoprotein or multidrug resistance associated protein

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Jung Ah; Lee, Jae Tae; Yoo, Jung Ah [School of Medicine, Kyungpook National University, Daegu (Korea, Republic of)] (and others)

    2005-02-15

    {sup 99m}Tc-sestamibi(MIBI) and {sup 99m}Tc-tetrofosmin have been used as substrates for P-glycoprotein (Pgp) and multidrug resistance associated protein (MRP), which are closely associated with multidrug resistance of the tumors. To understand different handling of radiotracers in cancer cell lines expressing Pgp and MRP, we compared cellular uptakes of {sup 99m}Tc-MIBI and {sup 99m}Tc-tetrofosmin. The effects of cyclosporin A (CsA), well-known multidrug resistant reversing agent, on the uptake of both tracers were also compared. HCT15/CL02 human colorectal cancer cells for Pgp expressing cells, and human non-small cell lung cancer A549 cells for MRP expressing cells, were used for in vitro and in vivo studies. RT-PCR, western blot analysis and immunohistochemistry were used for detection of Pgp and MRP. MDR-reversal effect with CsA was evaluated at different drug concentrations after incubation with MIBI or tetrofosmin. Radioactivities of supernatant and pellet were measured with gamma well counter. Tumoral uptake of the tracers were measured from tumor bearing nude mice treated with or without CsA. RT-PCR, western blot analysis of the cells and immunochemical staining revealed selective expression of Pgp and MRP for HCT15/CL02 and A549 cells, respectively. There were no significant difference in cellular uptakes of both tracers in HCT15/CL02 cells, but MIBI uptake was slightly higher than that of tetrofosmin in A549 cells. Co-incubation with CsA resulted in a increase in cellular uptakes of MIBI and tetrofosmin. Uptake of MIBI or tetrofosmin in HCT15/CL02 cells was increased by 10-and 2.4-fold, and by 7.5 and 6.3-fold in A549 cells, respectively. Percentage increase of MIBI was higher than that of tetrofosmin with CsA for both cells ({rho} < 0.05). In vivo biodistribution study showed that MIBI (114% at 10 min, 257% at 60 min, 396% at 24C min) and tetrofosmin uptake (110% at 10 min, 205% at 60 min, 410% at 240 min) were progressively increased by the time, up to

  8. Age-related normal uptake of {sup 99m}Tc-MDP in the lower spine

    Energy Technology Data Exchange (ETDEWEB)

    Sundkvist, G.M.G. [Dept. of Clinical Physiology, Centralsjukhuset, Kristianstad (Sweden); Lilja, B. [Dept. of Clinical Physiology, Universitetssjukhuset MAS, Malmoe (Sweden); Mattsson, S. [Dept. of Radiation Physics, Universitetssjukhuset MAS, Malmoe (Sweden)

    1997-03-01

    Aim: The uptake of {sup 99m}Tc-MDP in the lower spine was quantified in connection with ordinary bone scintigraphy. Methods: Fifty-seven women and seventy-two men (aged 20-69 y), with no signs of metabolic or malignant disease and no history of back pain or atraumatic fractures, were included in the study. Results: The women showed an increase in {sup 99m}Tc-MDP uptake from the age of 20 y to about 45 y with a decrease from the time of menopause. In contrast to women, the uptake in men showed a slight decrease from the age of 20 y to about 50 y and thereafter a more marked decrease was seen. The major difference between men and women is the high premenopausal uptake in the female skeleton. Conclusion: An understanding of age-related changes in {sup 99m}Tc-MDP uptake, which are indices of the skeletal metabolism, is of importance especially in prevention and treatment of osteoporosis, a common disorder in postmenopausal women. (orig.) [Deutsch] Ziel: In Verbindung mit einer routinemaessigen Skelettszintigraphie wurde die Speicherung von {sup 99m}Tc-MDP in der unteren Wirbelsaeule quantifiziert. Methoden: 57 Frauen und 22 Maenner (Alter von 20-69 Jahren) ohne metabolische oder boesartige Erkrankungen sowie ohne Rueckenschmerzen oder Wirbelsinterungen wurden in die Studien aufgenommen. Ergebnisse: Bei Frauen zeigte sich ein Anstieg der {sup 99m}Tc-MDP-Speicherung zwischen den Altersabschnitten 20 bis 45 Jahre und dann ein Abfall waehrend der Menopause. Im Gegensatz hierzu zeigte sich bei Maennern ein leichter Abfall der Speicherung vom 20. bis 50. Lebensjahr und danach eine noch staerkere Abnahme. Der Hauptunterschied zwischen den Geschlechtern ist die relativ hohe Speicherung im weiblichen Skelett praemenopausal. Schlussfolgerung: Das Verstaendnis der Altersbeziehungen der {sup 99m}Tc-MDP-Speicherung als Indikator des Knochenstoffwechsels ist fuer Praevention und Behandlung der postmenopausalen Osteoporose wichtig. (orig.)

  9. Technetium 99m-labeled annexin v scintigraphy of platelet activation in vegetations of experimental endocarditis

    Energy Technology Data Exchange (ETDEWEB)

    Rouzet, F.; Sarda-Mantel, L.; Le Guludec, D. [Nucl Med Serv, Grp Hosp Bichat Claude Bernard, AP-HP, Paris (France); Rouzet, F.; Sarda-Mantel, L.; LeGuludec, D. [Univ Denis Diderot Paris 7, UMR S773, Paris (France); Rouzet, F.; Sarda-Mantel, L.; Le Guludec, D. [INSERM, U773, Paris (France); Hernandez, M.D.; Louedec, L.; Michel, J.B. [Univ Paris 07, CHU Xavier Bichat, INSERM, U698, Paris (France); Hervatin, F. [CEA, DSV, DRM, SHFJ, Orsay (France); Lefort, A.; Fantin, B. [Univ Denis Diderot Paris 7, EA 3964, Paris (France); Duval, X. [Univ Denis Diderot Paris 7, INSERM, CIC 007, Paris (France); Duval, X. [Univ Denis Diderot Paris 7, AP-HP, Grp Hosp Bichat Claude Bernard, Ctr Invest Clin, Paris (France); Hernandez, M.D. [Univ Guadalajara, DeptPathol, Guadalajara 44430, Jalisco (Mexico)

    2008-07-01

    Background: The pathophysiology of infective endocarditis involves a pathogen/host tissue interaction, leading to formation of infected thrombotic vegetations. Annexin V is a ligand of phosphatidyl-serines exposed by activated platelets and apoptotic cells. Because vegetations are platelet-fibrin clots in which platelet pro-aggregant activity is enhanced by bacterial colonization, we investigated the ability of annexin V labeled with technetium {sup 99m}Tc ({sup 99m}Tc-ANX) to provide functional imaging of these vegetations in experimental models of infective endocarditis. This ability was assessed in rabbits and rats because of the different interest of these 2 species in preclinical analysis. Methods and Results: Non-bacterial thrombotic endocarditis was induced with the use of a catheter left indwelling through the aortic or tricuspid valve, and animals were injected with either a bacterial inoculum or saline. Scintigraphic investigations were performed 5 days later and showed a higher {sup 99m}Tc-ANX uptake by vegetations in infected versus non-infected animals (ratio,1.3 for in vivo acquisitions and 2 for autoradiography; P {<=} 0.0001 for all), whereas no significant uptake was present in controls. Right-sided endocarditis was associated with pulmonary uptake foci corresponding to emboli. Histological analysis of vegetations showed a specific uptake of {sup 99m}Tc-ANX at the interface between circulating blood and vegetation. In parallel, underlying myocardial tissue showed myocyte apoptosis and mucoid degeneration, without extracellular matrix degradation at this stage. Conclusions: {sup 99m}Tc-ANX is suitable for functional imaging of platelet-fibrin vegetations in endocarditis, as well as embolic events. {sup 99m}Tc-ANX uptake reflects mainly platelet activation in the luminal layer of vegetations. This uptake is enhanced by bacterial colonization. (authors)

  10. Clinical Significances of {sup 99m}Tc-DMSA Renal Scan in Patients with Acute Pyelonephritis

    Energy Technology Data Exchange (ETDEWEB)

    Mok, Cha Soo; Shin, Dong Jin; Choi, Ho Cheon; Kim, Gyeong Wook; Yi, Gang Wook; Choi, Dae Seop [Sang Ae General Hospital, Seoul (Korea, Republic of)

    1989-07-15

    Patients with acute pyleonephritis may present with a spectrum of clinical symptoms and signs. There are few noninvasive diagnostic studies, however to confirm or exclude this diagnosis. To evaluate the clinical utility of {sup 99m}Tc-DMSA renal scan in diagnosis of acute pyelonephritis, we performed {sup 99m}Tc-DMSA renal scan in 37 patients suspected with urinary tract infection. Simultaneously kidney ultrasonography was done in 21 patients diagnosed with acute pyelonephritis, clinically. And we performed the followup scan after treatment in two to six weeks. The results were as follows. 1) {sup 99m}Tc-DMSA renal scan disclosed single of multiple cortical defects and decreased radiouptake ratio of affected kidney in 23 patients among 25 patients diagnosed with acute pyleonephritis. 2) In the 21 patients with acute pyelonephritis, kidney ultrasonography showed abnormal findings in the 7 patients. And {sup 99m}Tc-DMSA renal scan disclosed abnormal cortical defects in this 7 patients also. 3) Between the patients with acute pyelonephritis and those with lower urinary tract infection, asymptomatic bacteriuria or pyuria, right-to-left radiouptake ratios (R/L ratio ) were significantly different(p<0.001). 4) In two to six weeks after antibiotic therapy, we performed followup {sup 99m}Tc-DMSA renal scan for 5 patients among 25 acute pyelonephritis patients. And we have found the improvement of cortical defects and the right-to-left radiouptake ratio. In conclusion, we thought that {sup 99m}Tc-DMSA renal scan should be useful in diagnosis of acute pyelonephritis and follow up examination.

  11. Phantom evaluation of simultaneous thallium-201/technetium-99m acquisition in single-photon emission tomography

    Energy Technology Data Exchange (ETDEWEB)

    Cao Zongjian [Department of Diagnostic Radiology, University of Maryland Medical Center, Baltimore, MD (United States); Chen, C.C. [Department of Diagnostic Radiology, University of Maryland Medical Center, Baltimore, MD (United States); Maunoury, C. [Department of Diagnostic Radiology, University of Maryland Medical Center, Baltimore, MD (United States); Holder, L.E. [Department of Diagnostic Radiology, University of Maryland Medical Center, Baltimore, MD (United States); Abraham, T.C. [Department of Diagnostic Radiology, University of Maryland Medical Center, Baltimore, MD (United States); Tehan, A. [Department of Diagnostic Radiology, University of Maryland Medical Center, Baltimore, MD (United States)

    1996-11-01

    This study investigated downscatter effects in cardiac single-photon emission tomographic studies with simultaneous thallium-201/technetium-99m acquisition, and evaluated a previously proposed subtraction technique for downscatter compensation. Ten studies were carried out with different defect sizes and locations and varying activity distributions using four energy windows: 70{+-}10% keV, 140{+-}10% keV, 100{+-}10% KeV, and 103{+-}16% keV. The subtraction technique used the 100- or 103-keV data to remove scattered {sup 99m}Tc counts from the 70-keV data. The size and contrast of infarcts in the dual-isotope 70-keV image were artificially decreased compared to those in the 140-keV image, caused by scattered {sup 99m}Tc counts that were comparable to the primary {sup 201}Tl counts in the 70-keV window. The subtraction technique produced larger defects and more heterogeneous activity in the myocardial wall in dual-isotope 70-keV images compared to the corresponding {sup 201}Tl-only images. These artifacts were caused by the markedly different spatial distributions of scattered {sup 99m}Tc counts in the 100-keV (or 103-keV) window as compared with the 70-keV window. It is concluded that scattered {sup 99m}Tc photons may cause overestimation of ischemia and myocardial viability in simultaneous dual-isotope patient studies. The proposed subtraction technique was inaccurate and produced image artifacts. Adequate downscatter compensation methods must be developed before applying simultaneous {sup 201}Tl/{sup 99m}Tc acquisition in clinical practice. (orig.). With 6 figs., 3 tabs.

  12. Labeling polypeptide with 99mTc and bioactivity get back

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A method for labeling polypeptide(insulin) with technetium-99(99mTc) was established without marked loss of biological activity. Following reduction of intrinsic disulfide bonds by mercaptoethanol and purification on a Sephadex G50 column,the polypeptide was labeled with 99mTc by transchelation from methylene diphosphonate (MDP). 99mTc labeled insulin was identified by thin layer chromatograph (TLC)and the change of blood sugar of mice injected, their hypoglycemic shock symptom was also observed. Six hours after labeling, the dissociation of labeled insulin was only 3%,From then on to 24h, there was no more dissociation. The blood sugar concentration of mice injected with the mercaptoethanol-reduced insulin was (5.0±3.2)μmol·L-1, while those injected with the original insulin was (l.4±l.2)μmol·L-1, the difference was significant(Q test, p<0.01). Blood sugar concentration of the mice was 0.3±0.2μmol·L-1for the labeled insulin, and was about the same with that for the original insulin.The labeling efficiency was 74.31% for the labeled insulin, whereas the original insuin cannot be labeled with 99mTc. The result suggests that while disulfide bonds of polypeptide were reduced by mercaptoethanol, it became free sulfhydryl group, and its bioactivity descended. Then free sulfhydryl group was chelated with 99mTc under mild condition, restablishing the disulfide bond, therefore, the bioactivity came back.The 99mTc-labeled insulin was stable during 24 h.

  13. Tc-99m Dimercaptosuccinic Acid (DMSA) Renal Scintigraphy in Patients with Acute Pyelonephritis

    Science.gov (United States)

    Lee, Kang Wook; Bin, Ki Tae; Jeong, Min Soo; Shong, Min Ho; Shin, Young Tai; Ro, Heung Kyu

    1995-01-01

    Objectives Recently, several authors reported that Tc-99m DMSA renal scan frequently showed cortical defects of the involved kidneys even in the patients with acute pyelonephritis who did not show abnormal findings in the ultrasonography and intravenous pyelography IVP). Methods In order to evaluate the utilities of Tc-99m DMSA renal scan and the clinical meanig of cortical defects in the Tc-99m DMSA renal scan of the patients with acute pyelonephritis, ninety two patients with acute pyelonephritis, from March 1991 to February 1994 in Chungnam National University Hospital(CNUH), were included in this study. Patients were subdivided as Group A:Patients showing normal Tc-99m DMSA renal scan(n=42) and Group B:Patients with definit cortical defects on the Tc-99m DMSA renal scan(n=50). We compared clinical characteristics such as age and sex, recurrency, duration of fever, bacterial culture study, incidence of renal insufficiency and the results of renal ultrasonography and intravenous pyelography between the two groups. Results Fifty four percents of 92 patients with acute pyelonephritis showed a significantly longer febrile period after admission, higher positive rates on the urine and blood culture studies and higher incidence of renal insufficiency than those of the Group A patients. Sixty nine percents of Group B patients showed normal results in ultrasonography or IVP study. Conclusions Tc-99m DMSA renal scan was a more sensitive imaging test than ultrasonography in kidneys and IVP to detect pyelonephritis lesions and may be useful to predict the patient group with a severe disease course. These patients may need more careful management and further studies to evaluate the possibility of complications. PMID:7626556

  14. Future of low specific activity molybdenum-99/technetium-99m generator.

    Science.gov (United States)

    Mushtaq, A

    2012-10-01

    In last few years, the shortage of molybdenum-99 (99Mo) was felt in the developed and developing countries hospitals, where diagnostic nuclear medicine is practiced. To overcome the shortage of 99Mo various routes of its production by accelerators and reactors generating low and high specific activity products have been planned. High specific activity 99Mo obtained by fission of uranium-235 (235U) has completely dominated in the manufacturing of technetium-99m (99mTc) generators in last 3-4 decades, but due to proliferation and dirty bomb, issues non fission routes of 99Mo production are emphasized. Future of low specific activity 99Mo is discussed.

  15. Juvenile Dermatomyositis Diagnosed by {sup 99m}Tc-HDP Three-phase Bone Scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Ja Hye; Song, Ho Chun; Yoo, Su Ung; Ha, Jung Min; Chong, A Ri; Oh, Jong Ryool; Min, Jung Joon; Bom, Hee Seung; Jeong, Eun Hui; Lee, Min Chul [Chonnam National University Hospital, Gwangju (Korea, Republic of)

    2009-12-15

    Juvenile dermatomyositis is a common inflammatory muscle disease of childhood, characterized by weakness in proximal muscles and specific skin rash. In case of juvenile dermatomyositis without characteristic clinical features, non-invasive imaging tools such as {sup 99m}Tc-HDP three-phase bone scan are very helpful in diagnostic workup of myopathies. We report a case of 13-year old female with juvenile dermatomyositis, in which {sup 99m}Tc-HDP three-phase bone scan was useful in diagnosis and assessing therapy response.

  16. 99mTc-Labeled HYNIC-DAPI Causes Plasmid DNA Damage with High Efficiency

    OpenAIRE

    Joerg Kotzerke; Robert Punzet; Roswitha Runge; Sandra Ferl; Liane Oehme; Gerd Wunderlich; Robert Freudenberg

    2014-01-01

    (99m)Tc is the standard radionuclide used for nuclear medicine imaging. In addition to gamma irradiation, (99m)Tc emits low-energy Auger and conversion electrons that deposit their energy within nanometers of the decay site. To study the potential for DNA damage, direct DNA binding is required. Plasmid DNA enables the investigation of the unprotected interactions between molecules and DNA that result in single-strand breaks (SSBs) or double-strand breaks (DSBs); the resulting DNA fragments ca...

  17. Performance tests on column materials for {sup 99}Mo-{sup 99m}Tc generator

    Energy Technology Data Exchange (ETDEWEB)

    Sombrito, E.Z.; Bulos, A.D.; Tangonan, M.C. [Chemistry Research Section, Atomic Research Div., Philippine Nuclear Research Inst., Quezon (Philippines)

    1998-10-01

    To meet the need of producing a {sup 99}Mo-{sup 99m}Tc generator, based on low specific activity reactor-produced {sup 99}Mo, different procedures for preparing zirconium molybdate gels were tested. Performance tests were done on molybdate gel columns prepared using the procedures developed by Vietnam and China, and recently, on a polyzirconium compound (PZC) prepared in Japan. The conditions for the batch drying of a large volume of the gel material were studied as well as the conditions in preparing a column to concentrate technetium-99m. The performance of PZC sample as column material for the generator was also evaluated. (author)

  18. Homogeneous SLOWPOKE reactors for Mo-99/Tc-99m production in North America

    Energy Technology Data Exchange (ETDEWEB)

    Hilborn, J.W., E-mail: hilbovanw@sympatico.ca [Deep River, Ontario (Canada); Bonin, H.W. [Royal Military College of Canada, Kingston, Ontario (Canada)

    2014-07-01

    The 15 month shutdown of NRU in 2009 - 2010 caused an overall isotope shortage of approximately 30%; and in North America, the annual Tc-99m demand decreased from an estimated 20 million unit doses to about 15 million unit doses. Mo-99/Tc-99m is produced from HEU targets, irradiated in NRU for 11 days, and after chemical removal of uranium it is shipped to Nordion in Kanata, Ontario. Nordion further purifies the material and sends it to Lantheus Medical Imaging in the USA for manufacture of Mo-99 generators, which are then distributed to hundreds of hospital radiopharmacies throughout North America. One other American company, Covidien, manufactures and distributes Mo-99 generators like Lantheus, but they import bulk Mo-99 from Europe or South Africa. At the hospitals, Tc-99m is chemically extracted daily from the Mo-99 generators and loaded into syringes for immediate clinical use. Fortuitously, the 66 hour half-life of Mo-99 allows the replenishment of Tc-99m in the generator over a growth period of about 20 hours; and a generator can be 'milked' daily for up to two weeks. A more efficient model is the direct production and distribution of Tc-99m unit doses to regional hospitals from 10 'industrial' radiopharmacies located at existing licensed reactor sites in North America. A 20 kW homogeneous SLOWPOKE reactor at each site would deliver 15 litres of irradiated uranyl sulphate fuel solution daily to industrial-scale hot cells for extraction of Mo-99, which would be incorporated in large Mo-99/Tc-99m generators for extraction of Tc-99m five days a week; and the Low Enriched Uranium (LEU) would be recycled. Each automated hot-cell facility would be designed to load up to 7,000 Tc-99m syringes daily, for courier delivery to all of the Nuclear Medicine hospitals within a 3 hour average range by road transport. Typically, the delivered doses would be in the range 10 to 30 mCi. Assuming an average unit dose of 25 mCi at the hospital and 5 x 52

  19. Biodistribution of technetium-99m pertechnetate after total colectomy in rats

    Energy Technology Data Exchange (ETDEWEB)

    Meneses Rego, Amalia Cinthia; Alcantara Oliveira Ramalho, Rachel; Tabosa Egito, Eryvaldo Socrates; Araujo-Filho, Irami; Medeiros Azevedo, Italo [Postgraduate Program in Health Sciences, Federal University of Rio Grande do Norte (UFRN), Ave. Miguel Alcides Araujo 1889, Natal-RN 59078-270 (Brazil); Palestro, Christopher J. [Division of Nuclear Medicine and Molecular Imaging, North Shore Long Island Jewish Health System, Manhasset and New Hyde Park, NY (United States); Medeiros, Aldo Cunha, E-mail: aldom@uol.com.b [Postgraduate Program in Health Sciences, Federal University of Rio Grande do Norte (UFRN), Ave. Miguel Alcides Araujo 1889, Natal-RN 59078-270 (Brazil)

    2010-12-15

    This study evaluated the effects of total colectomy on the biodistribution of technetium-99m pertechnetate ({sup 99m}TcO{sub 4}{sup -}) on the 28th postoperative day in rats. Samples of several organs were harvested for counting the percent of injected radioactivity/g of tissue (%ATI/g). The %ATI/g in colectomy rats was higher in the stomach and ileum than in sham and controls (p<0.05). Increase in mucosa and muscularis size of ileum was observed. Colectomy was associated with lower biodistribution in bladder and thyroid, T3, and T4, than in controls.

  20. Evaluation of regenerative capacity after kidney ischemic/reperfustion injury using 99mTc-DMSA

    Energy Technology Data Exchange (ETDEWEB)

    Kwak, W. J.; Kim, J. W.; Park, K. M.; Lee, S. W.; Ahn, B. C.; Lee, J. T.; Yoo, J. S. [Kyungpook National University School of Medicine, Daegu (Korea, Republic of)

    2007-07-01

    Acute renal failure can be caused by a reduced renal blood flow induced because of ischemic injury. The damaged kidney can be completely restored in structure and function. {sup 99m}Tc-DMSA binds to cortical tubules in kidney and its uptake has been suggested to indicate function of cortical mass. Herein, the generative capacity of kidney after bilateral or unilateral ischemia/reperfusion (I/R) injury was evaluated non-invasively by scintigraphic imaging. Three different animal models were used. One or both kidneys of mice were subjected ischemic for 30 min for unilateral or bilateral I/R model, respectively. In third model, one kidney was excised and the other kidney was subjected ischemic for 30 min to give nephrectomy model. At 1 hr, 1 d, 3 d, 1 w, 2 w, 3 w after reperfusion, {sup 99m}Tc-DMSA (27.7 MBq) was injected via tail vein. After 3 hr, the mice were scanned for 30 min with pinhole equipped gamma camera. The ratio of ROI counts of kidney to total counts was calculated. In unilateral I/R mouse, the {sup 99m}Tc-DMSA uptake of injured kidney was decreased continuously up to 3 w (13.9 to 7.7%), while uptake in normal kidney is slowly increased. In case of nephrectomy model, {sup 99m}Tc-DMSA uptake of injured kidney was rapidly restored within 1 w after I/R operation (8.5 to 30%). Bilateral model showed reduced {sup 99m}Tc-DMSA uptake at 1 d, but total uptake in both I/R kidney was also increase up to 30% after 1 w and the uptake was maintained up to 3 w. In unilateral model, the {sup 99m}Tc-DMSA uptake of injured kidney kept decreasing up to 3 w while normal kidney showed increased {sup 99m}Tc-DMSA uptake. The restoration of I/R kidney was not observed within 3 w. However, in case of animal models which have only I/R kidneys such as bilateral and nephrectomy models, the {sup 99m}Tc-DMSA uptake was restored within 1 w and the excised kidney size was also normal in contrast to much smaller I/R kidney of unilateral model.

  1. Extrarenal abnormalities in Tc-99m-DTPA renal blood flow studies

    Energy Technology Data Exchange (ETDEWEB)

    Shih, W.J.; Domstad, P.A.; DeLand, F.H.

    1985-01-01

    The authors observed extrarenal abnormalities during renal flow scintigraphy and retrospectively reviewed 90 patient studies to determine the types and frequencies of such abnormal findings. For each routine Tc-99m-DTPA renal flow study, they obtained nine 2-second sequential images, which included the heart, abdominal aorta, spleen and kidneys. Eighty abnormalities, observed in 62 patients, were divided into three categories: aortic, 37 cases; splenic, 40 cases; and miscellaneous, 3 cases. Other correlative studies including Tc-99m sulfur colloid-spleen scintigraphy, ultrasonography (US), CT, aortography, and surgical and/or autopsy findings were available for corroboration in 56 of 80 lesions.

  2. 99mTc-albumin can replace 125I-albumin to determine plasma volume repeatedly

    DEFF Research Database (Denmark)

    Bonfils, Peter K; Damgaard, Morten; Stokholm, Knud H

    2012-01-01

    OBJECTIVE: Plasma volume assessment may be of importance in several disorders. The purpose of the present study was to compare the reliability of plasma volume measurements by technetium-labeled human serum albumin ((99m)Tc-HSA) with a simultaneously performed plasma volume determination with iod......OBJECTIVE: Plasma volume assessment may be of importance in several disorders. The purpose of the present study was to compare the reliability of plasma volume measurements by technetium-labeled human serum albumin ((99m)Tc-HSA) with a simultaneously performed plasma volume determination...

  3. Milking technique of sup(99m)Tc generators and labeling efficiencies

    Energy Technology Data Exchange (ETDEWEB)

    Salehi, N.; Guignard, P.A. (Royal Melbourne Hospital, Parkville (Australia). Dept. of Nuclear Medicine)

    1985-05-01

    Increased levels of /sup 99/Tc in generator produced sup(99m)Tc have an adverse effect on the labelling efficiency of red blood cells and human serum albumin. A two-step milking technique in which the first 1-2 ml of eluate is discarded has been found to produce higher and more constant labelling efficiency of lymphocytes and platelets than a one-step procedure. Binding efficiency of platelet-rich plasma and lymphocytes with sup(99m)Tc is greater in the two-step technique. High activity concentration in the eluate for critical labelling is between 1.5-3 ml.

  4. sup 99m Tc-sucralfate scintigraphy in inflammatory bowel disease

    Energy Technology Data Exchange (ETDEWEB)

    Mortensen, P.B.; Lech, Y.; Moeller-Petersen, J.; Vilien, M.; Fallingborg, J. (Aalborg Sygehus (Denmark)); Ekelund, S. (Copenhagen County Hospital (Denmark))

    1989-01-01

    Technetium-99m-labelled albumin-sucralfate was orally administered to 11 patients (Crohn's disease, 8; ulcerative colitis, 3) and 3 healthy volunteers. Serial scintigraphy was performed, and scintigraphic interpretations were compared with radiographic end endoscopic findings in an open study. It was not possible in any patient to relate the scintigraphic findings to the localizations of inflammatory bowel disease, nor was it possible to distinguish the scans in the patients from the scans of the healthy volunteers. It is concluded the {sup 99m}Tc-albumin-sucralfate scintigraphy is of no value in the detection of inflammatory bowel disease. 8 refs.

  5. Improved method to label beta-2 agonists in metered-dose inhalers with technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Ballinger, J.R.; Calcutt, L.E.; Hodder, R.V.; Proulx, A.; Gulenchyn, K.Y. (Ottawa Civic Hospital, Ottawa (Canada). Div. of Nuclear Medicine and Respiratory Unit)

    1993-01-01

    Labelling beta-2 agonists in a metered-dose inhaler (MDI) with technetium-99m allows imaging of the deposition of the aerosol in the respiratory tract. We have developed an improved labeling method in which anhydrous pertechnetate is dissolved in a small volume of ethanol, diluted with a fluorocarbon, and introduced into a commercial MDI. Imaging the MDI demonstrated that the [sup 99m]Tc was associated with the active ingredient, not just the propellant. The method has been used successfully with salbutamol and fenoterol MDIs and should be directly applicable to other MDIs which contain hydrophilic drugs. (Author).

  6. A computerized compact module for separation of (99m)Tc-radionuclide from molybdenum.

    Science.gov (United States)

    Chattopadhyay, Sankha; Barua, Luna; De, Anirban; Saha Das, Sujata; Kuniyil, Remashan; Bhaskar, Partha; Pal, Sasanka Shekhar; Sarkar, Sishir Kumar; Das, Malay Kanti

    2012-11-01

    An automated closed cycle module for the separation and recovery of various isotopes, radioactive or non-radioactive, using solvent extraction and column chromatography techniques, and in particular, for separation and recovery of (99m)Tc from low-medium specific activity (99)Mo, is described. The module may also be applicable for separation of (99m)Tc produced in a cyclotron. The module is safe and reliable to avoid human interference and hazards posed by handling of radioactive and hazardous chemicals. The entire system of automation includes a user-friendly PC based graphical user interface (GUI) that actually supervises the process via an embedded system based electronic controller.

  7. Detection of acute cerebral ischaemia with Tc-99m apcitide scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Tepmongkol, S. [Nuclear Medicine Division, Department of Radiology, Faculty of Medicine, Chulalongkorn University, Rama IV Road, Bangkok 10330 (Thailand)

    2002-10-01

    The established indication for Tc99m apcitide scintigraphy is for detecting deep venous thrombosis. However, due to its mechanism of binding to GP IIb/IIIa receptors on activated platelets, it can be used to image acute cerebral thrombosis. I report a patient with an acute ischaemic stroke, with right leg swelling, referred for Tc99m apcitide scintigraphy to show of deep venous thrombosis. There was no abnormal uptake in the legs but there was in the left parieto-occipital region. This correlated with the clinical and CT data, indicating an acute ischaemic stroke in this area. (orig.)

  8. Bone marrow uptake of {sup 99m}Tc-MIBI in patients with multiple myeloma

    Energy Technology Data Exchange (ETDEWEB)

    Fonti, R. [Medicina Nucleare, Facolta di Medicina, Univ. Federico II, Naples (Italy); Centro per la Medicina Nucleare C.N.R., Cattedra di Medicina Nucleare, Napoli (Italy); Del Vecchio, S.; Zannetti, A.; Di Gennaro, F.; Pace, L.; Salvatore, M. [Centro per la Medicina Nucleare C.N.R., Cattedra di Medicina Nucleare, Napoli (Italy); De Renzo, A.; Catalano, L.; Califano, C.; Rotoli, B. [Cattedra di Ematologia, Dipt. di Medicina Clinica e Sperimentale, Napoli (Italy)

    2001-02-01

    In a previous study, we showed the ability of technetium-99m methoxyisobutylisonitrile ({sup 99m}Tc-MIBI) scan to identify active disease in patients with multiple myeloma (Eur J Nucl Med 1998; 25: 714-720). In particular, a semiquantitative score of the extension and intensity of bone marrow uptake was derived and correlated with both the clinical status of the disease and plasma cell bone marrow infiltration. In order to estimate quantitatively {sup 99m}Tc-MIBI bone marrow uptake and to verify the intracellular localization of the tracer, bone marrow samples obtained from 24 multiple myeloma patients, three patients with monoclonal gammopathy of undetermined significance (MGUS) and two healthy donors were studied for in vitro uptake. After centrifugation over Ficoll-Hypaque gradient, cell suspensions were incubated with {sup 99m}Tc-MIBI and the uptake was expressed as the percentage of radioactivity specifically retained within the cells. The cellular localization of the tracer was assessed by micro-autoradiography. Twenty-two out of 27 patients underwent {sup 99m}Tc-MIBI scan within a week of bone marrow sampling. Whole-body images were obtained 10 min after intravenous injection of 555 MBq of the tracer; the extension and intensity of {sup 99m}Tc-MIBI uptake were graded using the semiquantitative score. A statistically significant correlation was found between in vitro uptake of {sup 99m}Tc-MIBI and both plasma cell infiltration (Pearson's coefficient of correlation r=0.69, P<0.0001) and in vivo score (Spearman rank correlation coefficient r=0.60, P<0.01). No specific tracer uptake was found in bone marrow samples obtained from the two healthy donors. Micro-autoradiography showed localization of {sup 99m}Tc-MIBI inside the plasma cells infiltrating the bone marrow. Therefore, our findings show that the degree of tracer uptake both in vitro and in vivo is related to the percentage of infiltrating plasma cells which accumulate the tracer in their inner

  9. Padronização do método para cálculo da captação renal absoluta do99mTc-DMSA em cria Standardization of a method to calculate absolute renal uptake of 99mTc-DMSA in children

    Directory of Open Access Journals (Sweden)

    Carla Rachel Ono

    2006-02-01

    Full Text Available OBJETIVO: O trabalho teve por objetivo padronizar o método e estabelecer valores normais da captação renal absoluta do99mTc-DMSA em crianças. MATERIAIS E MÉTODOS: Vinte e duas crianças (idade de 7 meses a 10 anos; média de 4,5 anos sem doença renal prévia foram submetidas a cintilografia renal estática com 99mTc-DMSA. Dezoito apresentavam ultra-sonografia, uretrocistografia miccional, "clearance" de creatinina e padrão visual da cintilografia renal estática normais. Quatro crianças foram excluídas por não terem completado ou por apresentarem redução do "clearance" de creatinina. A captação absoluta de DMSA (DMSA-Abs foi calculada como a porcentagem da atividade administrada retida em cada rim após seis horas da administração do radiofármaco. RESULTADOS: Os valores de DMSA-Abs foram de 21,8% ± 3,2% para o rim direito e de 23,1% ± 3,3% para o rim esquerdo. Os valores da captação absoluta não mostraram correlação com a idade dos pacientes estudados, apesar da tendência de aumento do "clearance" de creatinina com a idade. CONCLUSÃO: A definição de valores normais da DMSA-Abs permite o emprego deste parâmetro na avaliação inicial e acompanhamento de doenças renais, principalmente em pacientes com acometimento bilateral ou com rim único (nos quais a função diferencial direita X esquerda tem valor limitado.OBJECTIVE: To standardize a method and determine normal values for absolute renal uptake of 99mTc-DMSA in children with normal creatinine clearance. MATERIALS AND METHODS: Twenty-two children (between 7 months and 10 years of age; mean 4.5 years without clinical evidence of renal disease were studied using 99mTc-DMSA scintigraphy. Eighteen had normal renal ultrasonography, micturating urethrocystography, creatinine clearance and visual interpretation of the scintigraphy with 99mTc-DMSA. Four children were excluded, one with incomplete creatinine clearance and three due to reduction in the creatinine clearance

  10. Evaluation of renal allografts using {sup 99m} Tc mononuclear leukocytes; Avaliacao de transplantes renais utilizando-se {sup 99m} Tc-leucocitos mononucleares

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Sergio Augusto Lopes de; Martins, Flavia Paiva Proenca; Carvalho, Antonio Carlos Pires; Gutfilen, Bianca [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina. Dept. de Radiologia]. E-mail: sergioalsouza@ufrj.br; Goncalves, Renato Torres; Pontes, Daniela Salomao [Hospital Universitario Clementino Fraga Filho, Rio de Janeiro, RJ (Brazil). Servico de Nefrologia; Fonseca, Lea Mirian Barbosa da [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina. Dept. de Medicina Nuclear

    2004-02-01

    Renal allograft acute rejection must be promptly diagnosed since its reversibility is related to the readiness in which treatment is initiated. The aim of this study was: to establish a quantitative method to evaluate kidney rejection and acute tubular necrosis (Attn); to assess the potential role of {sup 99m} Tc-mononuclear leukocytes scintigraphy in the diagnosis of renal rejection and differential diagnosis of Attn. One hundred and sixty studies were performed in 80 renal transplant patients at the first and fifth day after transplantation. Autologous cells were used for labeling. Images were obtained at 30 minutes, 3 hours and 24 hours after intravenous administration of 444 MBq (12 mCi) of labeled cells. There was abnormal labeled cells uptake in 27 of 31 cases of rejection and in 6 of 8 cases of Attn. The results of each patient were compared with clinical findings. Doppler scanning detected 18 of 31 cases of rejection. Rejection diagnosis sensitivity and specificity rates using scintigraphy were 87.1 per cent and 100 per cent, respectively, and 58.1 per cent and 100 per cent, respectively using ultrasound. Renal biopsy was performed in eight patients which demonstrated seven cases of rejection and one case of ATN. These results suggest that {sup 99m} Tc-mononuclear leukocytes imaging may be useful in the early diagnosis of rejection and in the differential diagnosis of ATN. (author)

  11. /sup 99m/pertechnetate uptake in the transplanted kidney. [/sup 99m/Tc, /sup 133/Xe, /sup 131/I, /sup 203/Hg

    Energy Technology Data Exchange (ETDEWEB)

    Aquino, H.C.; Preston, D.F.; Luke, R.G.

    1971-01-01

    Acute renal failure is a common complication of kidney transplantation. The major causes are acute tubular necrosis (ATN), arterial or venous thrombosis, rejection, ureteral obstruction, and extravasation. Each situation requires a different treatment and demands prompt diagnosis to prevent loss of the graft and patient morbidity or mortality. The clinical problem is further complicated by the possible coincidence of more than one of these pathologies, in particular the development of graft rejection superimposed on ATN in the post-transplant period. The diagnostic studies used in this differential diagnosis may include retrograde ureteral catheterization, renal arteriography, open or closed renal biopsy, isotope studies with /sup 133/xenon, /sup 131/I-hippuran, /sup 203/Hg-chlormerodrin, and more recently /sup 99m/pertechnetate. Only the latter methods with hippuran, chlormerodrin, and pertechnetate avoid direct manipulation of the graft or its artery or ureter with the inherent risks of such procedures. We present results of serial studies of sodium /sup 99m/pertechnetate photoscanning(Tc scan) in 38 renal homografts. In some studies computer determined graphs of renal radioactivity versus time (Tc renogram) were obtained.

  12. Scintigraphic head-to-head comparison between {sup 99m}Tc-WBCs and {sup 99m}Tc-LeukoScan in the evaluation of inflammatory bowel disease: a pilot study

    Energy Technology Data Exchange (ETDEWEB)

    Stokkel, M.P.M.; Reigman, H.I.E.; Pauwels, E.K.J. [Leiden University Medical Centre, Department of Nuclear Medicine, Leiden (Netherlands)

    2002-02-01

    Scintigraphy with technetium-99m labelled white blood cells (WBCs) is routinely used in our hospital for the assessment of inflammatory bowel disease (IBD). The main disadvantages of this diagnostic tool are its time-consuming nature and the handling of blood itself. {sup 99m}Tc-LeukoScan is a relatively new, easily prepared agent that is used for the detection of osteomyelitis. To assess its value in IBD, a scintigraphic head-to-head comparison was performed between {sup 99m}Tc-LeukoScan and {sup 99m}Tc-WBCs. {sup 99m}Tc-LeukoScan scintigraphy was performed in six patients with clinically active IBD and increased uptake on {sup 99m}Tc-WBC images. The interval between the scintigraphic studies ranged from 2 to 7 days, and endoscopy was subsequently performed to confirm active IBD. In three out of six patients with increased uptake on the {sup 99m}Tc-WBC scans, {sup 99m}Tc-LeukoScan images showed very discreet activity in the bowel, but the sites did not correspond with the inflammation sites seen on {sup 99m}Tc-WBC scintigraphy and found at endoscopy. In the other three patients, {sup 99m}Tc-LeukoScan scintigraphy revealed a physiological distribution but no abnormalities. In conclusion, {sup 99m}Tc-LeukoScan is not an alternative agent for the assessment of IBD. A prospective study is not justified owing to the false-negative results. (orig.)

  13. Evaluation of adenosine preconditioning with {sup 99m}Tc-His{sub 10}-annexin V in a porcine model of myocardium ischemia and reperfusion injury: preliminary study

    Energy Technology Data Exchange (ETDEWEB)

    Ye Fei [Department of Cardiology, Nanjing First Hospital Affiliated to Nanjing Medical University, 68 Changle Road, Nanjing 210006 (China); Fang Wei [Cardiovascular Institute and Fuwai Hospital, No. 167 Bei-Li-Shi-Lu, Beijing 100037 (China); Wang Feng, E-mail: fengwang1972cn@gmail.co [Department of Nuclear Medicine, Nanjing First Hospital Affiliated to Nanjing Medical University, 68 Changle Road, Nanjing 210006 (China); State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, College of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093 (China); Hua Zichun [State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, College of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093 (China); Wang Zizheng [Department of Nuclear Medicine, Nanjing First Hospital Affiliated to Nanjing Medical University, 68 Changle Road, Nanjing 210006 (China); Yang Xiang [State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, College of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093 (China)

    2011-05-15

    Purpose: The goal of this study was to evaluate the feasibility of {sup 99m}Tc-His{sub 10}-annexin V for the detection of acute myocardial cell death and to assess the effect of adenosine preconditioning in a porcine model of myocardium ischemia and reperfusion injury (RI). Materials and Methods: {sup 99m}Tc-His{sub 10}-annexin V was prepared by one-step direct labeling, and RCP and radiostability were tested. The binding of {sup 99m}Tc-His{sub 10}-annexin V to apoptosis was validated in vitro using camptothecin-induced Jurkat cells. In vivo biodistribution was determined in mice by the dissection method. Ischemia of 20-30 min was induced by balloon occlusion of the epicardial coronary artery of the porcine model (n=14). Adenosine was infused intravenously in six pigs before coronary occlusion. {sup 99m}Tc-His{sub 10}-annexin V (n=12) was injected intravenously at 1 h after reperfusion. SPECT/CT was acquired at 3 h postinjection. Myocardial perfusion imaging (MPI) with {sup 99m}Tc-MIBI was also performed 1 day after His{sub 10}-annexin V imaging. Cardiac tissues were analyzed postmortem using hematoxylin-and-eosin and TUNEL staining. Caspase-3 activity was measured to confirm the presence of apoptosis. Results: {sup 99m}Tc-His{sub 10}-annexin V had a RCP >98% and high stability 2 h after radiolabeling; it could bind to apoptotic cells with high affinity. Biodistribution of {sup 99m}Tc-His{sub 10}-annexin V showed a predominant uptake in the kidney and relatively low uptake in the myocardium, liver and gastrointestinal tract; rapid clearance from blood and kidney was observed. In the untreated group, intense uptake of His{sub 10}-annexin V was visualized in the defect which was shown in MPI, whereas in the adenosine group a mild uptake of {sup 99m}Tc-His{sub 10}-annexin was found in the risk area which showed no defects in the {sup 99m}Tc-MIBI image. TUNEL staining and activated caspase-3 confirmed the ongoing apoptosis in RI. Adenosine preconditioning significantly

  14. Hot spot(s) of the lung in technetium-99m albumin colloid liver-spleen scintigraphy: case report

    Energy Technology Data Exchange (ETDEWEB)

    Shih, W.J.; Brandenburg, S.; Coupal, J.J.; Sullivan, J.D.; Beeler, J.A.; Magoun, S.; Ryo, U.Y.

    1988-06-01

    The authors replaced /sup 99m/Tc albumin colloid for /sup 99m/Tc sulfur colloid as a radiopharmaceutical for liver-spleen imaging and found two instances of hot spot(s) in the lung. The preparation procedure of albumin colloid is easier and more convenient as compared to that of sulfur colloid. Whereas replacement of /sup 99m/Tc sulfur colloid by /sup 99m/Tc albumin colloid is inevitable, it should be emphasized that one should avoid blood withdrawal in the syringe containing albumin colloid to prevent formation of clot(s) during the venous puncture for /sup 99m/Tc albumin colloid.

  15. [The diagnostic value of 99mTc-MDP bone scan and computed tomography for bone metastases of breast cancer: a systematic review].

    Science.gov (United States)

    Yu, Yanxia; Kuang, Anren

    2014-06-01

    According to the inclusion and exclusion criteria, we searched for relevant original articles in some big Chinese and English databases. The qualities of the studies were evaluated with QUADAS quality assessment tool. A software program, Meta-disc, was used to obtain the pooled estimates and heterogeneity test for sensitivity, specificity, SROC curve, and so on. Finally the 17 article were included. On a per-patient basis, the pooled sensitivity (SEN), specificity (SPE), positive likelihood ratio (LR+), negative likelihood ratio (LR-), diagnostic odds ratio (DOR), Q*, SROC area under curve for 99mTc-MDP bone scan and computed tomography (CT) were 0. 87 and 0.99, 0.81 and 0.98, 3.88 and 13.86, 0.2 and 0.03, 27.73 and 612.17, 0.8418 and 0.9732, 0.9097 and 0.9952, respectively. On a per-focus basis, the pooled SEN, SPE, LR+, LR-, DOR, Q*, SROC area under curve for 99mTc-MDP bone scan was 0.86, 0.97, 13.32, 0.16, 102.4, 0.8944, 0.9528, respectively. For CT, only 1 article were included. This paper demonstrate: whether 99mTc-MDP or CT both have high diagnostic efficiency for bone metastase of breast cancer.

  16. Lung and renal uptake of techneticum Tc 99m sulphur colloid related to disseminated intravascular coagulation

    Energy Technology Data Exchange (ETDEWEB)

    Jelle Teertstra, H.; Verdegaal, W.P.; Ras, G.J.

    1985-01-01

    In addition to a recently published case study, we present another three cases in which we observed both lung and renal uptake of technetium Tc 99m sulphur colloid which was related to a period of disseminated intravascular coagulation. Being familiar with this relationship may influence the diagnosis and course of the illness in certain patients.

  17. Structural Requirements For In Vivo Detection of Cell Death with 99mTc-Annexin V

    OpenAIRE

    2005-01-01

    99mTc-annexin V is used to image cell death in vivo via high-affinity binding to exposed phosphatidylserine. We investigated how changes in membrane binding affinity, molecular charge, and method of labeling affected its biodistribution in normal mice and its uptake in apoptotic tissues.

  18. Evaluation of Tc-99m leukocyte scan in the diagnosis of acute appendicitis

    Energy Technology Data Exchange (ETDEWEB)

    Butler, J.A.; Marcus, C.S.; Henneman, P.L.; Inkelis, S.H.; Wilson, S.E.

    1987-05-01

    A new /sup 99m/Tc Microlite leukocyte scan was performed in 38 patients to assess its value in the diagnosis of acute appendicitis. Autologous leukocytes are labeled with /sup 99m/Tc by inducing phagocytosis of /sup 99m/Tc albumin microcolloid particles. The advantages of this test over the standard indium-111 scan include superior imaging capability, a marked reduction (greater than 75%) in the radiation dose, and performance of the test including labeling, in less than 3 hr. Imaging is performed at 5-90 min postinjection of labeled cells. There were 19 male and 19 female patients with ages ranging from 10 to 80 years, in whom the diagnosis of appendicitis was indeterminate on clinical examination. Of the 13 of the 38 patients (34%) who came to surgery 12 had acute appendicitis. The WBC scan correctly identified 10 of the 12 cases of appendicitis. There were two false-negative studies. In the nonoperative group of 25 patients admitted for observation, 21 studies were reported as negative and four identified other sites of inflammation. All patients with a negative study have remained asymptomatic on follow-up. With a sensitivity of 83% (10/12) and a specificity of 100% (26/26) the /sup 99m/Tc leukocyte scan provides a rapid and highly accurate method for diagnosis of appendicitis in this preliminary study of patients with equivocal clinical exams.

  19. Technetium-99m-sestamibi/pertechnetate subtraction scintigraphy vs ultrasonography for preoperative localization in primary hyperparathyroidism

    Energy Technology Data Exchange (ETDEWEB)

    Berczi, C.; Lukacs, G.; Balazs, G. [Department of Surgery, University of Debrecen (Hungary); Mezosi, E.; Bajnok, L. [1. Department of Internal Medicine, University of Debrecen (Hungary); Galuska, L.; Varga, J. [Department of Nuclear Medicine, University of Debrecen (Hungary)

    2002-03-01

    A prospective study was performed to evaluate the efficacy of technetium-99m-sestamibi and technetium-99m-pertechnetate subtraction scanning and US for imaging parathyroid glands in primary hyperparathyroidism. Sixty-three patients were surgically treated for primary hyperparathyroidism (HPT). Preoperative scintigraphy and US were performed in all cases. Bilateral neck exploration was carried out on each patient. Results of radionuclide studies and US were compared with surgical and histological findings. In 57 patients with primary HPT the radionuclide scanning gave true-positive results. Four false-negative and two false-positive scintigrams were obtained. The sensitivity and the positive predictive value (PPV) of scintigraphy were 93 and 97%, respectively. Forty-one cases were correctly localized by the US. Seventeen US results were false negative and five were false positive. The sensitivity and the PPV for US were 71 and 89%, respectively. There was a statistically significant difference between the sensitivity of the scintigraphy compared with the US (p=0.001). Sensitivities of radionuclide scans and US were higher for adenomas (100 and 83%) than for hyperplastic glands (75 and 40%). The sensitivity of technetium-99m-sestamibi and technetium-99m-pertechnetate subtraction scintigraphy was significantly higher compared with US. This sensitive method could help surgeons in performing a rapid and directed parathyroidectomy. (orig.)

  20. Role of 99mTc-ECD SPECT in the Management of Children with Craniosynostosis

    Directory of Open Access Journals (Sweden)

    Mayadhar Barik

    2014-01-01

    Full Text Available Purpose of the Report. There is a paucity of data on correlation of various imaging modalities with clinical findings in craniosynostosis. Moreover, no study has specifically reported the role of Tc99m-ECD SPECT in a large number of subjects with craniosynostosis. Materials and Methods. We prospectively analyzed a cohort of 85 patients with craniosynostosis from year 2007 to 2012. All patients underwent evaluation with Tc99m-ECD SPECT and the results were correlated with radiological and surgical findings. Results. Tc99m-ECD SPECT revealed regional perfusion abnormalities in the cerebral hemisphere corresponding to the fused sutures preoperatively that disappeared postoperatively in all the cases. Corresponding to this, the mean mental performance quotient (MPQ increased significantly P<0.05 postoperatively only in those children with absent perfusion defect postoperatively. Conclusions. Our study suggests that early surgery and release of craniosynostosis in patients with preoperative perfusion defects (absent on Tc99m-ECD SPECT study are beneficial, as theylead to improved MPQ after surgery.

  1. Tc-99m pyrophosphate scanning after ischemic exercise in McArdle's disease

    Energy Technology Data Exchange (ETDEWEB)

    Uno, Hideaki; Kawano, Keizo; Yukawa, Susumu; Nomoto, Hiroshi (Wakayama Medical Coll. (Japan))

    1982-03-01

    In order to clarify the mechanism of muscle contracture induced by ischemic exercise in a patient with McArdle's disease, bone scanning with Tc-99m pyrophosphate was performed. The clinical diagnosis was established in the patient based on the biochemical examinations of skeletal muscle biopsy. Ischemic exercise was done initially on the left forearm and then 20 hours later on the right forearm. Two hours later, 15 mCi of Tc-99m pyrophosphate was infused through the left antecubital vein. Exactly 4 hours later, a conventional bone scanning was carried out. In the patient with McArdle's disease, muscle contracture developed in both forearms during the ischemic exercise. Conventional bone scanning showed increased Tc-99m pyrophosphate labeling of the right forearm muscles at 2 hours after ischemic exercise. However, increased labeling of the left forearm muscles was not found at 22 hours after ischemic exercise. In the control, no muscle contracture developed during ischemic exercise and bone scan showed no increase in Tc-99m pyrophosphate labeling in the antebrachial region. These findings suggest that the basis of muscle contracture appears to be an increased concentration of Ca in muscle cells due to a failure of sarcoplasmic reticulum to reaccumulate Ca at ischemic exercise.

  2. Technetium-99m scintimammography in the diagnosis of malignant breast tumors

    Directory of Open Access Journals (Sweden)

    Jauković Ljiljana

    2004-01-01

    Full Text Available Background. Technetium-99m (99mTc tetrofosmin scintigraphy is a new imaging method for the diagnosis of various malignancies, such as lung, thyroid, and most frequently breast neoplasms. The aim of this study was to evaluate the diagnostic reliability of 99mTc-Tetrofosmin breast scintigraphy in the detection of malignant breast disease. Methods. 99mTc -Tetrofosmin scintimammography (SMM was performed in 28 patients with 30 breast lesions suspicious for malignancy. Standard mammography (MM was also done. After surgery, the results of SMM and MM were compared to definitive histopathological findings as the "gold standard". After intravenous injection of radiotracer, SMM was performed in prone and supine views of the thorax, using large field-of-view Gamma camera. Results. The results of SMM were interpreted visually and semiquantitatively, and evaluated as positive or negative. Sensitivity, specificity and accuracy, positive (PPV and negative predictive value (NPV were obtained in relation to histopathology. After comparing the results of SMM and MM, SMM was proved more sensitive (95% for SMM vs. 80% for MM, while the specificity of both methods was similar. Conclusion. The results of this study indicate that the contribution of SMM as a nuclear medicine procedure has its place in the diagnostic protocol for patients suspected of malignant breast cancer.

  3. Tc-99m-labeled Rituximab for Imaging B Lymphocyte Infiltration in Inflammatory Autoimmune Disease Patients

    NARCIS (Netherlands)

    Malviya, G.; Anzola, K. L.; Podesta, E.; Lagana, B.; Del Mastro, C.; Dierckx, R. A.; Scopinaro, F.; Signore, A.

    2012-01-01

    The rationale of the present study was to radiolabel rituximab with 99m-technetium and to image B lymphocytes infiltration in the affected tissues of patients with chronic inflammatory autoimmune diseases, in particular, the candidates to be treated with unlabelled rituximab, in order to provide a r

  4. Biodistribution of the radiopharmaceutical technetium-99m-sodium phytate in rats after splenectomy

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Kercia Regina Santos Gomes; Acucena, Maria Kadja Meneses Torres; Villarim Neto, Arthur; Rego, Amalia Cinthia Meneses [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Centro de Ciencias da Saude; Bernardo-Filho, Mario [Universidade do Estado do Rio de Janeiro (UERJ), RJ (Brazil). Inst. de Biologia Roberto Alcantara Gomes. Dept. de Biofisica e Biometria; Azevedo, Italo M