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Sample records for 99m clearance curves

  1. Comparison of 99mTc-DTPA GFR and 99mTc-DTPA Renal Clearance%肾动态体外摄取法GFR与99mTc-DTPA肾清除率的对比研究

    Institute of Scientific and Technical Information of China (English)

    齐慧敏; 辛岗; 张春丽; 李驚子; 邓丽荣; 王海燕

    2002-01-01

    Objective To evaluate the two methods in GFR measurements. Methods By using Receiver Operating Characteristic (ROC) curve, likelihood ratio and regression analysis we compared 99mTc- DTPA GFR with clearance of 99mTc-DTPA (CL 99mTc). Results The 99mTc - DTPA GFR mean area under ROC curve was 0.79 mean sensitivity was 62%, mean specificity was 83.8%, r = 0.78, regression formula was Y = 8.5 + 0.76X. Conclusion The accuracy of 99mTc - DTPA was not so good.

  2. Effect of increased surface tension and assisted ventilation on /sup 99m/Tc-DTPA clearance

    International Nuclear Information System (INIS)

    Experiments were performed to determine the effects of conventional mechanical ventilation (CMV) and high-frequency oscillation (HFO) on the clearance of technetium-99m-labeled diethylenetriamine pentaacetate (/sup 99m/Tc-DTPA) from lungs with altered surface tension properties. A submicronic aerosol of /sup 99m/Tc-DTPA was insufflated into the lungs of anesthetized, tracheotomized rabbits before and 1 h after the administration of the aerosolized detergent dioctyl sodium sulfosuccinate (OT). Rabbits were ventilated by one of four methods: 1) spontaneous breathing; 2) CMV at 12 cmH2O mean airway pressure (MAP); 3) HFO at 12 cmH2O MAP; 4) HFO at 16 cmH2O MAP. Administration of OT resulted in decreased arterial PO2 (PaO2), increased lung wet-to-dry weight ratios, and abnormal lung pressure-volume relationships, compatible with increased surface tension. /sup 99m/Tc-DTPA clearance was accelerated after OT in all groups. The post-OT rate of clearance (k) was significantly faster (P less than 0.05) in the CMV at 12 cmH2O MAP [k = 7.57 +/- 0.71%/min (SE)] and HFO at 16 cmH2O MAP (k = 6.92 +/- 0.61%/min) groups than in the spontaneously breathing (k = 4.32 +/- 0.55%/min) and HFO at 12 cmH2O MAP (4.68 +/- 0.63%/min) groups. The clearance curves were biexponential in the former two groups. We conclude that pulmonary clearance of /sup 99m/Tc-DTPA is accelerated in high surface tension pulmonary edema, and this effect is enhanced by both conventional ventilation and HFO at high mean airway pressure

  3. Pulmonary clearance of 99mTc-DTPA aerosol in patients with progressive systemic scleroderma

    International Nuclear Information System (INIS)

    Alveolar epithelial permeability was assessed in 32 patients with progressive systemic scleroderma (PSS), using 99mTc-DTPA aerosol. Immediately after the inhalation of 99mTc-DTPA aerosol for 3 to 6 minutes under normal tidal breathing, lung was imaged sequentially for 30 minutes from the posterior by a gamma camera and exponential fitting was processed on the time activity curve. T1/2 (min) was used as a parameter for the evaluation of permeability of alveolar epithelium. Patients with collagen disease showed shorter T1/2 (T1/2=43.7±23.8 min) than the normal volunteers (T1/2=76.8±8.7 min). No significant difference was observed between patients with or without interstitial changes on the chest CT. Significant correlation was not observed between T1/2 and %VC or %DLco. In 8 cases, studies were repeated in the interval of 3 to 19 months. Improvement of T1/2 was seen in 4 cases, independent of CT findings. These results suggest that 99mTc-DTPA aerosol clearance study provides information independent from other lung examinations, and may be useful for the assessment of lung interstitial changes in patients with PSS. (author)

  4. Clearance of 99mTc-DTPA and experimentally increased alveolar surfactant content

    International Nuclear Information System (INIS)

    The authors measured clearance of 99mTc-labeled diethylenetriamine pentaacetic acid (99mTc-DTPA) in rabbits with experimentally increased alveolar surfactant content. In one group of animals, surfactant production was increased by treatment with ambroxol, and another group of animals was treated with tracheal instillation of natural surfactant. A group of untreated control animals and animals treated with instillation of saline were also studied. Clearance was measured during standard conditions of mechanical ventilation and during ventilation with large tidal volumes. In ambroxol- and surfactant-treated groups, clearance rate was reduced compared with untreated control animals. In contrast, clearance rate increased after saline instillation. The differences were observed at both modes of ventilation. The findings indicate that the pulmonary surfactant system is a rate-limiting factor for the clearance of 99mTc-DTPA and that the volume dependence of clearance is not explained by stretching of the alveolar wall only. 28 refs., 2 figs., 1 tab

  5. Hepatic clearance of {sup 99m}Tc-GSA in cases of postoperative biliary atresia. A retrospective comparison with hepatic clearance of {sup 99m}Tc-PMT

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    Yamazaki, Tetsuro; Yamada, Shogo [Tohoku Univ., Sendai (Japan). School of Medicine; Maruoka, Shin

    1998-11-01

    There are a few reports about usefulness of {sup 99m}Tc-GSA liver scintigraphy (GSA imaging) for the evaluation of postoperative state of biliary atresia. However, comparison of significance for the disease between GSA imaging and {sup 99m}Tc-PMT hepatobiliary imaging have not been reported. Fifty-six GSA imagings for 41 postoperative biliary atresia patients were reviewed and their scintigraphic findings and the functional parameters were compared with those obtained from {sup 99m}Tc-PMT hepatobiliary imaging. In quantitative analysis, although the hepatic clearance of both tracers calculated by graphical analysis (Patlak plot) were well correlated in most cases, a discrepancy was observed in several cases with jaundice. The authors conclude that the hepatic clearance of {sup 99m}Tc-GSA is a more accurate index of the hepatic reserve than that of {sup 99m}Tc-PMT which can be influenced by hyperbilirubinemia and that the combination of both examinations is helpful to evaluate development of liver injury in postoperative biliary atresia patients. (author)

  6. [Hepatic clearance of 99mTc-GSA in cases of postoperative biliary atresia--a retrospective comparison with hepatic clearance of 99mTc-PMT].

    Science.gov (United States)

    Yamazaki, T; Maruoka, S; Yamada, S

    1998-11-01

    There are a few reports about usefulness of 99mTc-GSA liver scintigraphy (GSA imaging) for the evaluation of postoperative state of biliary atresia. However, comparison of significance for the disease between GSA imaging and 99mTc-PMT hepatobiliary imaging have not been reported. Fifty-six GSA imagings for 41 postoperative biliary atresia patients were reviewed and their scintigraphic findings and the functional parameters were compared with those obtained from 99mTc-PMT hepatobiliary imaging. In quantitative analysis, although the hepatic clearance of both tracers calculated by graphical analysis (Patlak plot) were well correlated in most cases, a discrepancy was observed in several cases with jaundice. The authors conclude that the hepatic clearance of 99mTc-GSA is a more accurate index of the hepatic reserve than that of 99mTc-PMT which can be influenced by hyperbilirubinemia and that the combination of both examinations is helpful to evaluate development of liver injury in postoperative biliary atresia patients. PMID:9916401

  7. [Quantification of separate renal function using Tc 99m DTPA and Tc 99m DMSA. Correlations between individual isotopic data and creatinine clearance].

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    Chevet, D; Moisan, A; Le Pogamp, P; Le Cloirec, J; Wehbe, B; Herry, J Y

    1984-01-01

    The purpose of this prospective study (30 controls and 100 renal patients) is to compare one to each other the values of renal function measured with: 1--the DTPA 99mTc , 2--the DMSA 99mTc , and 3--the creatinine clearance. After the control values being established, correlations are done for the whole group (n = 130) between the creatinine clearance, the DTPA 99mTc clearance and the fixation rate of the DMSA 99mTc . The correlations obtained are:--Clcr Versus Cldtpa = 0,91.--Clcr Versus % Dmsa = 0,90. - Cldtpa Versus % Dmsa = 0,93. and indicate the two isotopic tests performed give similar quantitative results than creatinine clearance. Camera and computer allow to measure the separate renal function one by one, the two isotopic test giving similar values for each kidney. The results of these studies give a good concordance with the observed diseases, except for partial renal artery stenosis. The pyelic retention of DMSA 99mTc product, in condition of ureteral compression, gives a potential overestimation of this test and is a matter to debate. The extreme simplicity and facility of DMSA 99mTc procedures make this investigation accessible to any patient and give excellent morphological and functional tests. DTPA 99mTc clearances--global and separate kidney--give a more sensitive approach than DMSA 99mTc does because vascular captation , elimination rates could be focused. DTPA 99mTc procedure is to be used in patients with transplanted kidney, silent IVP kidney, contrast media contraindication or for exploring glomerular clearance without urine collection.

  8. Sidestream smoke inhalation decreases respiratory clearance of 99mTc-DTPA acutely

    International Nuclear Information System (INIS)

    The permeability of the alveolar-capillary barrier to an inhaled aerosol of technetium 99m labelled diethylenetriamine penta-acetate (99mTc-DTPA is used as an index of alveolar epithelial injury. Permeability is greatly increased in active smokers. The aim of this study was to determine the effect of sidestream smoke inhalation on permeability as this has not been described previously. Lung clearance of inhaled 99mTc-DTPA aerosol was measured in 20 normal non-smoking subjects before and after exposure to one hours sidestream smoke inhalation. Measured carbon monoxide (CO) levels rose to a maximum of 23.5 ±6.2 ppm from baseline values of 0.6±1.3 (p99mTc-DTPA clearance rose from baseline 69.1± 15.6 (mean ± to 77.4 ±17.8) after smoke exposure. No effect of 99mTc-DTPA scanning of sidestream smoke was demonstrated on lung function. It was concluded that low level sidestream smoke inhalation decreases 99mTc-DTPA clearance acutely in humans. The mechanism of this unexpected result is not established but may include differences in constituents between sidestream and mainstream smoke, alterations in pulmonary microvascular blood flow, or changes in surfactant due to an acute phase irritant response. 34 refs., 2 figs

  9. Relationship of end-expiratory pressure, lung volume, and /sup 99m/Tc-DTPA clearance

    International Nuclear Information System (INIS)

    We investigated the dose-response effect of positive end-expiratory pressure (PEEP) and increased lung volume on the pulmonary clearance rate of aerosolized technetium-99m-labeled diethylenetriaminepentaacetic acid (99mTc-DTPA). Clearance of lung radioactivity was expressed as percent decrease per minute. Base-line clearance was measured while anesthetized sheep (n = 20) were ventilated with 0 cmH2O end-expiratory pressure. Clearance was remeasured during ventilation at 2.5, 5, 10, 15, or 20 cmH2O PEEP. Further studies showed stepwise increases in functional residual capacity (FRC) (P less than 0.05) measured at 0, 2.5, 5, 10, 15, and 20 cmH2O PEEP. At 2.5 cmH2O PEEP, the clearance rate was not different from that at base line (P less than 0.05), although FRC was increased from base line. Clearance rate increased progressively with increasing PEEP at 5, 10, and 15 cmH2O (P less than 0.05). Between 15 and 20 cmH2O PEEP, clearance rate was again unchanged, despite an increase in FRC. The pulmonary clearance of aerosolized /sup 99m/Tc-DTPA shows a sigmoidal response to increasing FRC and PEEP, having both threshold and maximal effects. This relationship is most consistent with the hypothesis that alveolar epithelial permeability is increased by lung inflation

  10. Pulmonary clearance of 99mTc-DTPA and 99mTc-albumin in rabbits with surfactant dysfunction and lung injury

    International Nuclear Information System (INIS)

    We measured the pulmonary clearance of inhaled 99mTc-DTPA and 99mTc-albumin in rabbits with surfacant dysfunction induced by dioctyl sodium sulphosuccinate and in rabbits with lung injury induced by oleic acid. After inhalation of 99mTc-albumin in ten animals, clearance of the tracer from the lungs was monitored for 90 min. The first 30 min was a control period. Dioctyl sodium sulphosuccinate was then administered in aerosol and after another 30 min oleic acid was injected intravenously. Ten other rabbits were given 99mTc-DTPA, and clearance was externally recorded for 60 min. Five animals inhaled detergent aerosol and five animals were given oleic acid intravenously after 30 min. Airway pressures, tidal volume, and arterial blood gases were measured before and after each intervention. The half-life of 99mTc-albumin in the lung was 442 ± 123 min during the control period, 363 ± 52 min after detergent administration, and 134 ± 18 min after oleic acid administration. The half-life of 99mTc-DTPA was 94 ± 16 min before and 10 ± 0.6 min after detergent administration and 75 ± 12 min before and 18 ± 1.8 min after oleic acid administration. Gas exchange was not affected by administration of dioctyl sodium sulphosuccinate but markedly impaired after injection of oleic acid. Compliance of the respiratory system remained unaffected by detergent but decreased after injection of oleic acid. The results indicate that the rate limiting factors for the alveolo-capillary transfer of 99mTc-albumin and 99mTc-DTPA are different. Surfactant dysfunction affects the transfer of 99mTc-DTPA but not 99mTc-albumin. (author)

  11. The effect of airway deposition on the assessment of lung injury by 99mTc-DTPA clearance

    International Nuclear Information System (INIS)

    The 99mTc-DTPA aerosol inhalation method permits detection of pulmonary epithelial damage. We investigated one of several problems, airway deposition of inhaled aerosol, on the assessment of pulmonary epithelial permeability in healthy nonsmokers and patients with interstitial lung diseases. We used the rate constant of pulmonary 99mTc-DTPA clearance curve, k, as a parameter of the epithelial permeability. The alveolar-peripheral airway deposition of aerosol was estimated by the duplicated inhalation method, which we newly developed. The mean k in patients with interstitial lung disease (2.52±0.72%/min, n=8; pc) was higher in patients with interstitial lung disease (4.08±1.63%/min; pc in both groups (pc obtained among the subjects (r=0.951; p99mTc-DTPA aerosol inhalation method although the correction was significant in the individual subjects. (author)

  12. Effects of sustained exercise on pulmonary clearance of aerosolized 99mTc-DTPA

    International Nuclear Information System (INIS)

    The effects of intensive prolonged exercise on the pulmonary clearance rate of aerosolized 99mTc-labeled diethylenetriaminepentaacetate (99mTc-DTPA) and pulmonary mechanics were studied in seven healthy nonsmoking volunteers. 99mTc-DTPA clearance and pulmonary mechanics (lung volumes and compliance) were assessed before and after 75 min of constant-load exercise performed on a treadmill, corresponding to 75% of maximal O2 uptake. Because both clearance measurements were made in similar conditions of pulmonary blood flow, respiratory rate, and tidal volume, changes in clearance rate can be assumed to represent changes of alveolar epithelial permeability. After exercise, total, apical, and basal clearance were significantly increased (P less than 0.01, 0.05, and 0.05, respectively) and the increases in total clearance and tidal volume observed during exercise were significantly correlated (P less than 0.05). In contrast, no significant change was found in pulmonary mechanics. These results show that prolonged intensive exercise induces an increase in epithelial permeability, which appears to be related to the mechanical effects of sustained increased ventilation. Because no change was evidenced in pulmonary volumes or in lung elasticity, our results suggest that this increase may result from alteration of the intercellular tight junctions rather than from a surfactant deficiency

  13. Effect of raised thoracic pressure and volume on 99mTc-DTPA clearance in humans

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    Nolop, K.B.; Maxwell, D.L.; Royston, D.; Hughes, J.M.

    1986-05-01

    Although positive airway pressure is often used to treat acute pulmonary edema, the effects on epithelial solute flux are not well known. We measured independently the effect of 1) positive pressure and 2) voluntary hyperinflation on the clearance of inhaled technetium-99m-labeled diethylenetriaminepentaacetic acid (/sup 99m/Tc-DTPA) in six nonsmokers and six smokers. Lung volumes were monitored by inductance plethysmography. Each subject was studied in four situations: 1) low end-expiratory volume (LO-), 2) low volume plus 9 cmH2O continuous positive airway pressure (LO+), 3) high end-expiratory volume (HI-), and 4) high volume plus continuous positive airway pressure (HI+). The clearance half time of 99mTc-DTPA for the nonsmokers decreased from 64.8 +/- 7.0 min (mean +/- SE) at LO- to 23.2 +/- 5.3 min at HI- (P less than 0.05). Positive pressure had no synergistic effect. The mean clearance half time for the smokers was faster than nonsmokers at base line but unaffected by similar changes in thoracic volume and pressure. We conclude that, in nonsmokers, positive airway pressure increases /sup 99m/Tc-DTPA clearance primarily through an increase in lung volume and that smokers are immune to these effects.

  14. Reverse lung clearance of Tc-99m DTPA induced by postural changes

    International Nuclear Information System (INIS)

    The lung clearance of Tc 99m DTPA is generally attributed to the degree of distension of the interepithelial junctions and thus of the alveoli. To assess this hypothesis a submicronic aerosol of Tc 99m DTPA (0.5 μ mass median diameter) was inhaled by eight non smoking volunteers placed in left lateral decubitus (LLD) where the alveoli of the left lung are compressed and those of the right lung expanded. The lung radioactivity was followed with a gamma camera 15 minutes in LLD, then 15 minutes in right lateral decubitus (RLD) to reverse the relative distension of the alveoli. Regional clearances were computed on each lung divided in three horizontal slices. These results suggest that alveolar distension has a major role in the DTPA clearance which is relatively independent of the initial deposition of the aerosol

  15. Lung clearance of 99mTc-DTPA in systemic lupus erythematosus

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    P.T.R. Dalcin

    2002-06-01

    Full Text Available The early demonstration of lung involvement in systemic lupus erythematosus (SLE patients is a difficult but important task. In the present study we attempted to identify abnormalities in pulmonary clearance of 99mTc-DTPA in SLE, correlating their clearance data with clinical findings and disease activity. Forty-six consecutive SLE patients with and without active disease (LACC score and 30 normal volunteers were studied. All subjects were submitted to pulmonary scintigraphy with 99mTc-DTPA to evaluate the pulmonary clearance, and to a chest X-ray, and SLE patients were submitted to tests of disease activity, spirometry, arterial blood gases and tests to assess acute-phase proteins. Pulmonary clearance was faster in SLE patients with active disease when compared to normal controls [half-life of 67.04 min (51.52-82.55 min in active SLE versus 85.87 min (78.85-92.87 min in controls, P<0.05] and there was a higher frequency of abnormal clearance rates in patients with active disease (11 of 26 patients, 42.3% when compared with SLE patients without disease activity (2 of 20 patients, 10% (P = 0.04. A significant correlation was observed between the clearance rates and cough (P<0.05, but not between the clearance rates and dyspnea symptoms or radiological findings, duration of SLE disease, antinuclear antibody titers and patterns, C-reactive protein or anti-double stranded DNA antibodies. We conclude that the pulmonary clearance of 99mTc-DTPA is increased in SLE patients with active disease.

  16. Lung clearance of 99m Tc- DTPA in systemic lupus erythematous

    International Nuclear Information System (INIS)

    The early demonstration of lung involvement in systemic lupus erythematosus (SLE) patients is a difficult but important task. In the present study we attempted to identify abnormalities in pulmonary clearance of 99 mTc-DTPA in SLE, correlating their clearance data with clinical findings and disease activity. Forty-six consecutive SLE patients with and without active disease (LACC score) and 30 normal volunteers were studied. All subjects were submitted to pulmonary scintigraphy with 99 mTc-DTPA to evaluate the pulmonary clearance, and to a chest X-ray, and SLE patients were submitted to tests of disease activity, spirometry, arterial blood gases and tests to assess acute-phase proteins. Pulmonary clearance was faster in SLE patients with active disease when compared to normal controls [half-life of 67.04 min (51.52-82.55 min) in active SLE versus 85.87 min (78.85-92.87 min) in controls, P<0.05] and there was a higher frequency of abnormal clearance rates in patients with active disease (11 of 26 patients, 42.3%) when compared with SLE patients without disease activity (2 of 20 patients, 10%) (P = 0.04). A significant correlation was observed between the clearance rates and cough (P<0.05), but not between the clearance rates and dyspnoea symptoms or radiological findings, duration of SLE disease, antinuclear antibody titers and patterns, C-reactive protein or anti-double stranded DNA antibodies. We conclude that the pulmonary clearance of 99 m Tc-DTPA is increased in SLE patients with active disease. (author)

  17. Lung clearance of {sup 99m} Tc- DTPA in systemic lupus erythematous

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    Dalcin, P.T.R; Barreto, S.S.M.; Xavier, R.M.; Brenol, J.C.T. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Hospital de Clinicas. Dept. de Medicina Interna; Cunha, R.D.; Marroni, B.J. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Hospital de Clinicas. Servico de Medicina Nuclear]. E-mail: rxavier@hcpa.ufrgs.br

    2002-06-01

    The early demonstration of lung involvement in systemic lupus erythematosus (SLE) patients is a difficult but important task. In the present study we attempted to identify abnormalities in pulmonary clearance of 99 mTc-DTPA in SLE, correlating their clearance data with clinical findings and disease activity. Forty-six consecutive SLE patients with and without active disease (LACC score) and 30 normal volunteers were studied. All subjects were submitted to pulmonary scintigraphy with 99 mTc-DTPA to evaluate the pulmonary clearance, and to a chest X-ray, and SLE patients were submitted to tests of disease activity, spirometry, arterial blood gases and tests to assess acute-phase proteins. Pulmonary clearance was faster in SLE patients with active disease when compared to normal controls [half-life of 67.04 min (51.52-82.55 min) in active SLE versus 85.87 min (78.85-92.87 min) in controls, P<0.05] and there was a higher frequency of abnormal clearance rates in patients with active disease (11 of 26 patients, 42.3%) when compared with SLE patients without disease activity (2 of 20 patients, 10%) (P = 0.04). A significant correlation was observed between the clearance rates and cough (P<0.05), but not between the clearance rates and dyspnoea symptoms or radiological findings, duration of SLE disease, antinuclear antibody titers and patterns, C-reactive protein or anti-double stranded DNA antibodies. We conclude that the pulmonary clearance of 99 m Tc-DTPA is increased in SLE patients with active disease. (author)

  18. Effect of inspiratory resistance and PEEP on /sup 99m/Tc-DTPA clearance

    International Nuclear Information System (INIS)

    Experiments were performed to determine the effect of markedly negative pleural pressure (Ppl) or positive end-expiratory pressure (PEEP) on the pulmonary clearance (k) of technetium-99m-labeled diethylenetriaminepentaacetic acid (/sup 99m/Tc-DTPA). A submicronic aerosol containing /sup 99m/Tc-DTPA was insufflated into the lungs of anesthetized intubated sheep. In six experiments k was 0.44 +/- 0.46% (SD)/min during the initial 30 min and was unchanged during the subsequent 30-min interval [k = 0.21 +/- 12%/min] when there was markedly increased inspiratory resistance. A 3-mm-diam orifice in the inspiratory tubing created the resistance. It resulted on average in a 13-cmH2O decrease in inspiratory Ppl. In eight additional experiments sheep were exposed to 2, 10, and 15 cmH2O PEEP (20 min at each level). During 2 cmH2O PEEP k = 0.47 +/- 0.15%/min, and clearance increased slightly at 10 cmH2O PEEP [0.76 +/- 0.28%/min, P less than 0.01]. When PEEP was increased to 15 cmH2O a marked increase in clearance occurred [k = 1.95 +/- 1.08%/min, P less than 0.001]. The experiments demonstrate that markedly negative inspiratory pressures do not accelerate the clearance of /sup 99m/Tc-DTPA from normal lungs. The effect of PEEP on k is nonlinear, with large effects being seen only with very large increases in PEEP

  19. [Evaluation on blood clearance and hepatic uptake of 99mTc-GSA in rats with blood flow conversion].

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    Ohno, K

    1997-05-01

    This study was aimed to clarify a contribution of the hepatic blood flow in hepatic accumulation of 99mTc-DTPA-galactosyl serum albumin (99mTc-GSA). The experiment was performed in rats by the blood flow conversion with an external scintillation gamma camera and laser doppler flowmeter. Rats were divided into 4 groups: hepatic artery ligation (HAL, n = 10), portal vein ligation (PVL, n = 8), both hepatic artery and portal vein ligation (HAL+PVL, n = 9), and control (CONT, n = 10) groups. The scintigraphic data were obtained in each group for 10 minutes after intravenous injection of 99mTc-GSA (50 micrograms/100 g B.W). The regions of interest were assigned over the heart and whole liver and the time activity curves (TAC) were generated. Five parameters of HH 4, LHL 4, KH1, KH2, KL, were calculated as blood clearance and hepatic accumulation from TAC in each rat. HH4 as blood clearance index in CONT, HAL, PVL and HAL+PVL was 0.58 +/- 0.04 (mean +/- SE), 0.63 +/- 0.04, 0.85 +/- 0.04, 0.97 +/- 0.001, respectively. HH 4 between CONT vs PVL, HAL+PVL was statistically significant (p hepatic uptake index in CONT, HAL, PVL and HAL+PVL was 0.96 +/- 0.001, 0.93 +/- 0.01, 0.71 +/- 0.07, 0.41 +/- 0.04, respectively. This parameter was also statistically significant between CONT vs PVL and HAL+PVL groups. Another parameter of KH1 for blood clearance and KL for hepatic uptake were also significant between CONT vs PVL and HAL+PVL groups. All parameters obtained in 99mTc-GSA study correlated well with the hepatic flow rate which was measured with a laser doppler flowmeter and reflected the reduction rate of the hepatic tissue blood flow 4 minutes after the ligation of target vessels. These result suggest that blood clearance and hepatic uptake of 99mTc-GSA are significantly affected by hepatic blood inflow. 99mTc-GSA scintigraphy may be useful in evaluating hepatic tissue blood flow. PMID:9226469

  20. Exercise increases the lung clearance of inhaled Tc-99m DTPA

    International Nuclear Information System (INIS)

    It has been suggested that blood flow have little effect on the lung clearance of Tc-99m DTPA which depends on the alveolar distension. However an increase of pulmonary blood flow, induces a recruitment of new vascular areas which can enhance this clearance. To assess this hypothesis the pulmonary apical and basal clearances of a sumicronic aerosol of Tc-99m DTPA were studied at rest and at exercise in seven non smoking volunteers in upright posture. As a matter of fact exercising upright is known to increase primarily the apical blood flow. After inhalation the subjects were seated on a bicycle their back against a gamma camera which was linked to a computer. The lung radioactivity was registered successively during a resting and an exercising period. At rest there was a gradient of clearance from the apex to the base of the lung, the apical clearance being significantly higher. At exercise (50 Watts, 60 c. min /sup -1/, 7 min.) this regional gradient was enhanced by a large and significant increase of the apical clearances (0.0340 min /sup -1/ +-0.0063 SD versus 0.00183 min /sup -1/ +- 0.074 SD at rest, n = 7, p < 0.01). By contrast the changes of the basal clearances were slight and unsignificant (0.0146 min /sup -1/ +- 0.0062 SD versus 0.0140 min /sup -1/ +- 0.0082 SD). Since exercise induces little distension of the apical alveoli but drastically enhances the apical blood flow, the observed increase of the apical lung clearance could be primarily attributed to the recruitment of new capillaries induced by exercise in the apices. This led to an increase of the surface area permeability product

  1. Clearance of technetium-99m-DTPA and HRCT findings in the evaluation of patients with Idiopathic Pulmonary Fibrosis

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    Karkavitsas Nikolaos

    2006-02-01

    Full Text Available Abstract Background Clearance of inhaled technetium-labeled diethylenetriamine pentaacetate (99mTc-DTPA is a marker of epithelial damage and an index of lung epithelial permeability. The aim of this study was to investigate the role of 99mTc-DTPA scan in patients with Idiopathic Pulmonary Fibrosis (IPF. Our hypothesis is that the rate of pulmonary 99mTc-DTPA clearance could be associated with extent of High Resolution Computed Tomography (HRCT abnormalities, cell differential of bronchoalveolar lavage fluid (BALF and pulmonary function tests (PFTs in patients with IPF. Methods We studied prospectively 18 patients (14 male, 4 female of median age 67yr (range 55–81 with histologically proven IPF. HRCT scoring included the mean values of extent of disease. Mean values of these percentages represented the Total Interstitial Disease Score (TID. DTPA clearance was analyzed according to a dynamic study using a Venticis II radioaerosol delivery system. Results The mean (SD TID score was 36 ± 12%, 3 patients had mild, 11 moderate and 4 severe TID. Abnormal DTPA clearance half-time (t1/2 Conclusion Our data suggest that 99mTc-DTPA lung scan is not well associated with HRCT abnormalities, PFTs, and BALF cellularity in patients with IPF. Further studies in large scale of patients are needed to define the role of this technique in pulmonary fibrosis.

  2. Respiratory clearance of 99mTc-DTPA and pulmonary involvement in sarcoidosis

    International Nuclear Information System (INIS)

    To investigate the relationships between the respiratory epithelial clearance of micronic aerosolized /sup 99m/Tc-DTPA (RC-DTPA) and pulmonary function, serum angiotensin-converting enzyme (SACE), and lymphocytic alveolitis in patients with sarcoidosis, RC-DTPA was measured in 49 nonsmokers with pulmonary sarcoidosis and 38 normal nonsmokers. Pulmonary involvement was evaluated on chest roentgenograms (type O = normal, type I = hilar adenopathies, type II = hilar adenopathies associated with parenchymal shadows, type III = parenchymal shadows without adenopathy) and by pulmonary function tests. Serum angiotensin-converting enzyme was determined, and a bronchoalveolar lavage was performed for alveolar lymphocyte differential counting (Ly%). RC-DTPA was increased (greater than or equal to 1.96%/min) in 12 of 31 patients with type II or III involvement but was normal in all 18 patients with type O or I involvement (p = 0.002). Patients with increased RC-DTPA had low FVC, TLC, FEV1, and resting Pao2 (p less than 0.05); resting and exercise AaPo2 were increased (p less than 0.05), but RC-DTPA correlated negatively with FEV1 (p less than 0.01), Pao2 at rest (p less than 0.005), and DLCO (p less than 0.05) and positively with resting and exercise AaPO2 (p less than 0.01). In patients with increased RC-DTPA (42 +/- 17%), Ly% did not differ from Ly% in patients with normal RC-DTPA (34 +/- 16%). SACE was increased in patients with increased RC-DTPA (56 +/- 26 U/ml versus 38 +/- 16 U/ml; p = 0.007) and correlated positively with RC-DTPA (p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

  3. The measurement of 99mTc-DTPA pulmonary clearance in normals, asymptomatic smokers and diabetic patients

    International Nuclear Information System (INIS)

    We measured pulmonary epithelial permeability by 99mTc-DTPA radioaerosol clearance in patients with diabetes and correlated with the presence of microangiopathy to understand the pathophysiology of pulmonary microangiopathy and evaluate 99mTc-DTPA radioaerosol clearance as a diagnostic test to assess pulmonary microangiopathy. We performed 99mTc-DTPA radioaerosol scan in 10 normal subjects, 10 asymptomatic smokers, 20 diabetic patients without history of smoking (10 with microangiopathy, 10 without microangiopathy). 99mTc-DTPA clearance half-time (T1/2) was calculated, then compared with the result of chest radiography and pulmonary function test. Chest radiography and pulmonary function test were normal in all subjects. There were no significant difference of clinical or laboratory characteristics between these groups except age. The diabetic patients with microangiophaty were significantly older (p1/2 of normal subjects and asymptomatic smokers were significantly different (65.2±23.7 min vs 39.6±9.8 min, p1/2 was 90.5±46.5 min and significantly delayed when compared with those of normals and asymptomatic smokers (p1/2 of diabetic patients without microangiopathy, 70.0±12.7 min, was not significantly different from those of normals or asymptomatic smokers (p>0.05). No significant correlation was found between the T1/2 and spirometric parameters including DLco, FVC, FEV1, FEV1/FVC (%) and FEF25-75% in all subjects, and between the T1/2 and duration of diabetes in diabetic patients. Eventhough the influence of age can't be excluded, delayed 99mTc-DTPA clearance half-time (T1/2) in diabetic patients with microangiopathy indicates decreased pulmonary capillary permeability as one of the pathophysiologic results of pulmonary microangiopaththy. Further studies are needed in larger number of age matched control and diabetic patients to evaluate the diagnostic efficacy

  4. Diagnostic accuracy of quantitative 99mTc-MIBI scintimammography according to ROC curve analysis

    Energy Technology Data Exchange (ETDEWEB)

    Kim, J. H.; Lee, H. K.; Seo, J. W.; Cho, N. S.; Cha, K. H.; Lee, T. H. [Gachon Medical College, Gil Medical Center, Inchon (Korea, Republic of)

    1998-07-01

    99mTc-sestamibi scintimammography (SMM) has been shown to be a useful diagnostic test in the detection of breast cancer and the receiver operating characteristic (ROC) curve analysis provides detailed information of a diagnostic test. The purpose of this study was to evaluate the feasibility and efficacy of quantitative indices of SMM in the detection of malignant breast lesions according to ROC analysis. Prone anterior, lateral planar and supine SPECT imagings were performed on 75 female patients (mean age=43.4 yr) with breast mass (size{>=}0.8cm) after intravenous injection of 20-30 mCi 99mTc-sestamibi. 45 Malignant (Invasive ductal ca(36), Inv lobular ca(5), Inv duc + lob (1), Inv tubular ca (3)) and 30 benign (fibroadenoma (13), fib cyst(12), Fat necrosis(3), papilloma(1), paraffinoma (1)) lesions were histologically proven. Data were analyzed by creating three regions of interest (ROIs) over designated areas: lesion, normal breast and right chest wall. Lesion to normal (L/NL) and lesion to chest wall (L/CW) ratios were calculated for each patient both on the planar and SPECT. The area under the ROC curve (AUC) was calculated and compared among four semiquantitative indices and an average scintimammographic index (SMM(mean)) from arithmatic mean. ROC curve analysis revealed planar L/N, SPECT L/N and L/CW ratios provide comparable better diagnostic accuracies for detection of breast cancer than planar L/CW ratio (p<0.05), respectively. For quantitative SMM of 75 lesions, malignancy rate was 60%, and Sensitivity, Specificity, Positive Predictive Value, Negative Predictive Value and Accuracy were 0.78, 0.77, 0.84, 0.72 and 0.77, respectively. Quantitative SMM is an useful objective method for differentiating malignant from benign breast lesions.

  5. Clearance of inhaled sup(99m)Tc DTPA from regions of the lung recently affected by pulmonary embolus

    International Nuclear Information System (INIS)

    Pulmonary emboli lead to regional limitation of pulmonary artery perfusion, often without affecting distribution of ventilation. We have studied the effect of this regional reduction of pulmonary artery perfusion on the integrity of epithelium of alveoli (and possibly bronchioli). Integrity of alveolar epithelium was assessed by measuring regional rates of clearance from lung to blood of an inhaled aerosol of a small molecular weight solute, sup(99m)Tc DTPA (technetium-99m diethylene-triamine-penta-acetate). Ten patients with pulmonary emboli were studied, where the diagnosis was made from 'mismatching' seen on ventilation (V) and perfusion (Q) lung scintigraphy. Rates of clearance of DTPA in regions with normal V/Q ratios were compared with embolized regions with high V/Q ratios. Clearance rates were increased in embolized regions (V/Q ratio > 1) : 2.59 +- (SD) 0.89%. min-1, compared with normal regions (V/Q ratio -1 (p<0.01). In repeat studies in nine patients (one died), after intervals between 2 and 12 weeks, the V/Q ratio of previously embolized regions decreased towards unity in all nine patients, suggesting resolution. The differences in clearance rates for DTPA between normal and embolized regions decreased in association with this return towards normal of V/Q ratios. We surmise that reduction in pulmonary artery perfusion which occurs in pulmonary embolic disease alters the integrity of the alveolar (and possibly bronchiolar) epithelium. This effect is largely reversible, recovering with return of pulmonary artery perfusion

  6. Comparison of /sup 99m/Tc-DTPA and /sup 113m/In-DTPA aerosol clearances in humans. Effects of smoking, hyperinflation, and in vitro oxidation

    Energy Technology Data Exchange (ETDEWEB)

    Nolop, K.B.; Maxwell, D.L.; Fleming, J.S.; Braude, S.; Hughes, J.M.; Royston, D.

    1987-11-01

    As an index of permeability of the alveolar epithelium, the clearance of an inhaled aerosol of /sup 99m/Tc-DTPA is increased in several disease states. However, the usefulness of the test to assess the severity of disease is limited because healthy smokers also have abnormally rapid rates of clearance. Because the stability of the /sup 99m/Tc-DTPA bond might be a contributory factor, we tested the affinity of /sup 99m/Tc for DTPA in vitro, and in groups of healthy smokers (n = 13) and nonsmokers (n = 7) we measured the clearances of /sup 99m/Tc-DTPA and /sup 113m/In-DTPA, which have a similar molecular shape and charge. In vitro, sodium hypochlorite or hydrogen peroxide released as much as 98% of free /sup 99m/Tc from the /sup 99m/Tc-DTPA complex. When incubated with human neutrophils stimulated with phorbol myristate acetate, between 4 and 7% of free /sup 99m/Tc-DTPA was released after 30 min, and 12% was released after 60 min. In vivo, the clearances of both /sup 99m/Tc-DTPA and /sup 113m/In-DTPA in the smokers (n = 13) were faster than in the nonsmokers (n = 7) (p less than 0.05). Within the smokers, the mean /sup 99m/Tc-DTPA clearance (T1/2 25 +/- 4 min) was faster than the mean /sup 113m/In-DTPA clearance (34 +/- 6 min), (p less than 0.05). For nonsmokers, the difference was smaller (T1/2 /sup 99m/Tc-DTPA, 56 +/- 6; T1/2 /sup 113m/In-DTPA, 62 +/- 6) and not significant. During hyperinflation, smokers (n = 8) and nonsmokers (n = 8) both demonstrated an increase in /sup 113m/In-DTPA clearance.

  7. Evaluation of novel cationic {sup 99m}Tc-nitrido complexes as radiopharmaceuticals for heart imaging: improving liver clearance with crown ether groups

    Energy Technology Data Exchange (ETDEWEB)

    Liu Shuang [School of Health Sciences, Purdue University, Lafayette, IN 47907 (United States)]. E-mail: lius@pharmacy.purdue.edu; He Zhengjie [School of Health Sciences, Purdue University, Lafayette, IN 47907 (United States); Hsieh, W.-Y. [School of Health Sciences, Purdue University, Lafayette, IN 47907 (United States); Kim, Young-Seung [School of Health Sciences, Purdue University, Lafayette, IN 47907 (United States)

    2006-04-15

    This report describes the evaluation of a series of novel cationic {sup 99m}Tc-nitrido complexes, [{sup 99m}TcN(DTC)(PNP)]{sup +} (DTC=crown ether-containing dithiocarbamates; PNP=bisphosphine), as potential radiotracers for myocardial perfusion imaging. Synthesis of cationic {sup 99m}Tc-nitrido complexes was accomplished in two steps according to literature methods. Biodistribution studies were performed in rats. Planar images of Sprague-Dawley rats administered with 15{+-}2 MBq of cationic {sup 99m}Tc radiotracer were obtained using a PhoGama large field-of-view Anger camera. Samples from both urine and feces were analyzed by a reversed-phase radio-HPLC method. Results from biodistribution studies showed that most of the cationic {sup 99m}Tc-nitrido complexes have a high initial heart uptake with a long myocardial retention. They also show a rapid clearance from the liver and lungs. Cationic complexes [{sup 99m}TcN(L2)(L6)]{sup +} and [{sup 99m}TcN(L4)(L6)]{sup +} show heart/liver ratios four to five times better than that of {sup 99m}Tc-sestamibi due to their much faster liver clearance. Their heart uptake and heart/liver ratio are comparable to that of {sup 99m}TcN-DBODC5 within the experimental error. These findings have been confirmed by the results from imaging studies. Radio-HPLC analysis of urine and feces samples indicated that there was very little metabolism of cationic {sup 99m}Tc-nitrido complexes in rats under anesthesia. The key finding of this study is that lipophilicity remains the most important factor affecting both heart uptake and target-to-background (T/B) ratios. Crown ethers are very useful functional groups to improve the liver clearance of cationic {sup 99m}Tc-nitrido complexes. It is the combination of the appropriate DTCs and bisphosphines that results in cationic {sup 99m}Tc-nitrido complexes with high heart uptake and fast clearance from the liver at the same time. The fast liver clearance of [{sup 99m}TcN(L2)(L6)]{sup +} and [{sup 99

  8. Measurement of Regional and Global Pulmonary Clearance of 99mTc-DTPA (Demethylamitriptylene-Acetate): An Index of Alveolar Epithelial Permeability

    International Nuclear Information System (INIS)

    The main purpose of this study has been introduction of a new method for alveoli-capillary permeability evaluation. Many reports pointed out to the altered transit of soluble particles through this barrier. From pathophysiological aspect the main interest is the elucidation of permeability's alteration in different pulmonary pathology. We decided to use for lung epithelial permeability measurements 99mTc-DTPA inhaled aerosols and sequential assessment of its lung clearance. The aerosols were obtained using oxygen flow nebulizers with aerosols' generators Ultra Vent (Malinkrodt) and Venticis II (CIS bio international) that enabled as to get submicron particles. Oxygen flow between 9 and 11 liters per minute was used. Optimum images were obtained with 1480 MBq of inhaled aerosols at least 2 to 3 minutes. DTPA that was used for aerosols labeling had been produced in our Department and the results were compared with DTPA provided by CIS bio international. High correlation between both agents was proven. During the whole study ex tamper prepared radiopharmaceuticals were used and quality control was done using paper chromatography method. Acquisition was done in sitting position with gamma camera interfaced to a ADAC and Scintiview. The measurements lasted for 20 minutes. Data were stored on 64x64 matrices. Regions of interest over both lungs were drown and each one was divided in three segments: apical, medial, and basal. Using computer program curves of 99mTc-DTPA lung clearance were derived. From the obtained time activity curves half-time of the global and the regional lung clearance was assessed. In the control group comprised of 32 healthy volunteers (non-smokers) we had got values, used after works as reference range. Our normal values for global clearance are: 68±5,5 min. for left whole lung, 68,1±6,5 min for right whole lung, and 49±7,7 min for apical, 66,9±8 min for middle, and 75,9±6,4 min basal regional lung clearance, and they are in keeping with the

  9. Stability, protein binding and clearance studies of [99mTc]DTPA. Evaluation of a commercially available dry-kit

    DEFF Research Database (Denmark)

    Rehling, M

    1988-01-01

    the quality of a commercial [99mTc]DTPA preparation (C.I.S., France) with reference to stability, protein binding and accuracy of the determined plasma clearance values as a measure of GFR. The stability of the preparations was studied by thin-layer chromatography, the in vitro protein binding by Sephadex...... filtration after incubation with human serum albumin and in vivo protein binding by filtration of human plasma. The accuracy of the plasma clearance values was investigated by comparison with the simultaneously measured plasma clearance of [51Cr]EDTA. There was no detectable free pertechnetate or hydrolysed...... reduced technetium in eight vials five and six hours after the preparation. The in vitro protein binding 10 (20), 120 and 300 min after the preparation of eight vials was 2.3% (0.8%), 0.2% and 0.1%, respectively. The in vivo protein binding in 12 patients 5, 90 and 180 min after the injection was 0.3%, 0...

  10. Hepatic clearance mechanism of /sup 99m/Tc-HIDA and its effect on quantitation of hepatobiliary function concise communication

    International Nuclear Information System (INIS)

    Parameters affecting the hepatobiliary clearance of /sup 99m/Tc N(2,6-dimethylphenylcarbamoylmethyl) iminodiacetic acid (Tc-HIDA) were evaluated in dogs. Competitive clearance studies were performed with Tc-HIDA after infusion to plasma saturation levels of an anion, sodium sulfobromophthalein (BSP), and a cation, oxyphenonium. The results demonstrated that Tc-HIDA is transported through hepatocytes by a carrier-mediated organic-anion pathway. The data are consistent with an alteration of the elimination kinetics of Tc-HIDA induced by elevations in the serum bilirubin level, and it is predicted that serum bilirubin at some increased concentration will dominate the distribution and elimination kinetics of Tc-HIDA independently of hepatobiliary status. A quantitative description of liver function in terms of regional distribution and elimination rate constants will require either a pharmacokinetic model that expressly includes the effects of bilirubin from transport binding sites, or the development of new hepatobiliary agents that use a different clearance mechanism from that used by bilirubin

  11. Clearance of technetium-99m-labeled DTPA in hyperthyroidism without clinical evidence of lung disease, and relation to pulmonary function

    International Nuclear Information System (INIS)

    The mechanisms of dyspnea and exercise intolerance have not been fully elucidated. We aimed to investigate the clearance rate of technetium-99m diethyltriaminepentaaceticacid (Tc-99m DTPA) from lungs in hyperthyroid patients without clinical evidence of lung disease and to explore the interactions between their Tc-99m DTPA radioaerosol lung scintigraphy, spirometric measurements, and the levels of thyroid hormones. We studied 19 hyperthyroid patients and 16 sex- and age-matched controls. Thyroid hormone levels were assessed. Spirometric lung function tests, diffusing capacity of the lung for carbon monoxide (DLCO) and the clearance rate of Tc-99m DTPA were performed in all participants. Ratio of DLCO value to the alveolar ventilation (DLCO/VA) and the means of half-time (T1/2) of Tc-99m DTPA clearance rate, which were used to evaluate alveolar-capillary membrane permeability, were calculated. There were no statistical differences between spirometric parameters (vital capacity (VC), force vital capacity (FVC), one second forced expiratory volume (FEV1)/FVC, mean forced expiratory flow during the middle of FVC (FEF 25-75)) of the two groups (p>0.05). Although the mean FEV1 level was significantly lower in the hyperthyroid patients than the control subjects (p1 was only less than 80 percent of the predicted value. No significant difference in the means of DLCO, DLCO/VA or T1/2 values of Tc-99m DTPA clearance was observed between the two groups (p>0.05). In hyperthyroid patients, there was a positive relation between DLCO/VA, DLCO/VA% and T1/2 values of Tc-99m DTPA clearance (p1/2 values of Tc-99m DTPA clearance in hyperthyroid group (p>0.05). We conclude that increased thyroid hormones have no effect on permeability of alveolar-capillary membrane in hyperthyroid patients. (author)

  12. Biological effects in lymphocytes irradiated with 99mTc: determination of the curve dose-response

    International Nuclear Information System (INIS)

    Biological dosimetry estimates the absorbed dose taking into account changes in biological parameters. The most used biological indicator of an exposition to ionizing radiation is the quantification of chromosomal aberrations of lymphocytes from irradiated individuals. The curves of dose versus induced biological effects, obtained through bionalyses, are used in used in retrospective evaluations of the dose, mainly in the case of accidents. In this research, a simple model for electrons and photons transports was idealized to simulate the irradiation of lymphocytes with 99m Tc, representing a system used for irradiation of blood cells. The objective of the work was to establish a curve of dose versus frequencies of chromosomal aberrations in lymphocytes of human blood. For the irradiation of blood samples micro spheres of human serum of albumin (HSAM) market with 99m Tc were used, allowing the irradiation of blood with different administered activities of 99m Tc, making possible the study the cytogenetical effects as a function of such activities. The conditions of irradiation in vivo using HSAM spheres marked with 99m Tc were simulated with MCNP 4C (Monte Carlo N-Particle) code to obtain the dose-response curve. Soft tissue composition was employed to simulate blood tissue and the analyses of the curve of dose versus biological effect showed a linear quadratic response of the unstable chromosomal aberrations. As a result, the response of dose versus chromosomal aberrations of blood irradiation with 99m Tc was best fitted by the curve Y=(8,99 ±2,06) x 1--4 + (1,24 ±0,62) x 10-2 D + (5,67 ± 0,64) x 10-2 D2. (author)

  13. 99mTc-HEPIDA hepatic clearance as a diagnostic tool: usefulness of plasma and hepatic clearance for assessment of hepatic parenchyma performance.

    Science.gov (United States)

    Frieske, Izabela; Surma, Marian J; Bieńkiewicz, Małgorzata; Białkowska-Warzecha, Jolanta; Liniecki, Julian; Kuydowicz, Jan; Kuśmierek, Jacek

    2003-01-01

    Plasma clearance of (99m)Tc-HEPIDA (Cl(Pl)) has been used for two decades for assessment of liver function in patients with diseases of this organ. A specific determination of (99m)Tc-HEPIDA liver clearance (Cl(Hp)) has been developed that provides more direct possibility to evaluate performance of liver parenchyma. Both tests have been studied in healthy volunteers of varying age (48 individuals) and in 83 patients with varying degree of liver damage. The liver damage has been evaluated on the basis of 5 biochemical tests (AspAT, ALAT, GGTP, bilirubine serum concentration, proteinogram) and a score system used for total impairment, which was calculated for each patient. Normal range of Cl(Pl) and Cl(Hp) was determined from a study on healthy individuals (volunteers). The results seem independent of age, but show sex differences. The following values (mean +/- SD) of Cl(Hp) were found in males and females of: (181 +/- 31) ml//min/1.73 m(2) and (158 +/- 22) ml/min/1.73m(2), and of Cl(Pl) were (224 +/- 33) ml/min/1.73 m(2) and (202 +/- 25) ml/min/1.73 m(2) respectively. Accepted lower boundaries of both quantities (mean -2SD) are 115 ml/min/1.73 m(2) and 150 ml/min/1.73 m(2) correspondingly. Negative correlation of individual values of both clearances in all patients with individual score of liver damage were highly significant and correlation coefficients obtained were higher for Cl(Hp) (r = -0.63) than those for Cl(Pl) (r= -0.56). Factorial analysis was performed with the intention of seeing which of the studied factors had the highest factor loading for parenchyma performance that was assumed as the common factor responsible for correlations. The highest value was obtained for hepatic clearance (Cl(Hp)) of (99m)Tc-HEPIDA. In conclusion this quantity seems highly promising as a clinically useful test for assessment of liver performance, both in screening for liver damage and for monitoring of organ conditions during therapy and follow-up of patients.

  14. Biological effects in lymphocytes irradiated with {sup 99m}Tc: determination of the curve dose-response; Efeitos biologicos em linfocitos irradiados com {sup 99m}Tc: determinacao da curva dose-resposta

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Romero Marcilio Barros Matias de

    2002-08-01

    Biological dosimetry estimates the absorbed dose taking into account changes in biological parameters. The most used biological indicator of an exposition to ionizing radiation is the quantification of chromosomal aberrations of lymphocytes from irradiated individuals. The curves of dose versus induced biological effects, obtained through bionalyses, are used in used in retrospective evaluations of the dose, mainly in the case of accidents. In this research, a simple model for electrons and photons transports was idealized to simulate the irradiation of lymphocytes with {sup 99m} Tc, representing a system used for irradiation of blood cells. The objective of the work was to establish a curve of dose versus frequencies of chromosomal aberrations in lymphocytes of human blood. For the irradiation of blood samples micro spheres of human serum of albumin (HSAM) market with {sup 99m} Tc were used, allowing the irradiation of blood with different administered activities of {sup 99m} Tc, making possible the study the cytogenetical effects as a function of such activities. The conditions of irradiation in vivo using HSAM spheres marked with {sup 99m} Tc were simulated with MCNP 4C (Monte Carlo N-Particle) code to obtain the dose-response curve. Soft tissue composition was employed to simulate blood tissue and the analyses of the curve of dose versus biological effect showed a linear quadratic response of the unstable chromosomal aberrations. As a result, the response of dose versus chromosomal aberrations of blood irradiation with {sup 99m} Tc was best fitted by the curve Y=(8,99 {+-}2,06) x 1-{sup -4} + (1,24 {+-}0,62) x 10{sup -2} D + (5,67 {+-} 0,64) x 10{sup -2} D{sup 2}. (author)

  15. Detection of impaired renal function. Is the modern serologic marker cystatin C more accurate than the {sup 99m}Tc-MAG{sub 3} clearance?

    Energy Technology Data Exchange (ETDEWEB)

    Reinhardt, M.J.; Weidling, H.; Breuel, H.P.; Biersack, H.J. [Klinik und Poliklinik fuer Nuklearmedizin, Univ. Bonn (Germany)

    2004-12-01

    Aim: Real function is usually determined by means of creatinine-clearance, and of serum Cystatin C, the latter with increasing frequency. The present study analyses, whether the diagnostic accuracy of {sup 99m}Tc-MAG{sub 3} clearance is comparable to that of these modern serologic methods. Patients, methods: 71 consecutive adult Caucasian patients (42 female, 29 male; age 50{+-}16 yrs., range 20-83) who were referred to a nuclear medicine department for determination of bilateral renal function with {sup 99m}Tc-MAG{sub 3} were included. Following sufficient hydration, 10 ml of blood were taken for determination of Cystatin C and creatinine in serum prior to i.v. injection of the radiotracer. According to the recommendations of the National Kidney Foundation, glomerular filtration rate (GFR) was calculated form serum creatinine using either Cockcroft and Gault and Modification of Diet in Renal Disease (MDRD) study equation. These estimates of GFR served as reference. Cystatin C is a low molecular protein produced by all nuclear cells and is eliminated to 85% by glomerular filtration. Analysis of {sup 99m}Tc-MAG{sub 3} clearance was performed by means of Bubeck's formula. Results: Linear regression analysis produced Pearson's correlation coefficients of r=0.68 and r=-0.69 for the comparison of either Cystatin C and {sup 99m}Tc-MAG{sub 3} clearance with the Cockcroft and Gault equation. The comparison of Cystatin C and {sup 99m}Tc-MAG{sub 3} clearance with MDRD study equation resulted in correlation coefficients of r=0.755 and r=-0.77. None of these differences were significant. The exclusion of renal impairment or the detection of an at least moderate renal impairment revealed again no significant differences between Cystatin C and {sup 99m}Tc-MAG{sub 3} clearance. Conclusions: Cystatin C and {sup 99m}Tc-MAG{sub 3} clearance are equally suited to exclude renal impairment or to detect a relevant renal impairment. Differences between both procedures are more

  16. [Radioisotopic quantification of kidney function using Tc-99m-DMSA. Comparison with creatinine clearance in children with a single kidney].

    Science.gov (United States)

    Baillet, G; Gagnadoux, M F; Mathonnat, F; de Vernejoul, P; Broyer, M

    1986-01-01

    To assess the accuracy of renal function quantification with Tc 99m-DMSA in children, we compared DMSA renal uptake and creatinine clearance in 16 cases of children with single kidney. The age of the patients ranged from two month to fourteen years. DMSA renal uptake was measured 7 hours after injection and was normalized in percent of the injected activity. A significant correlation was found between creatinine clearance and DMSA uptake (Pearson's r = 0.866, p less than 0.01). Normal creatinine clearance in children (80 to 120 ml/min-1 X 1.73 m-2) allowed determination of normal renal uptake (36 to 60%). This study indicates that in cases of asymmetrical renal impairment renal uptake reflects split renal creatinine clearance. Since the former is much easier to measure, DMSA should play an important role in the evaluation of differential renal function.

  17. Gfr estimation using 99mTc DTPA gates method for assessment of early diabetic nephropathy - a comparison with 24-hour creatinine clearance

    International Nuclear Information System (INIS)

    To correlate Gates glomerular filtration rate (GGFR) using technetium-99m diethylene triaminepentacetic acid (99mTc DTPA) with 24-hour creatinine clearance (CRCL) and to establish relationship with duration of diabetes in patients with early diabetic nephropathy. Study Design: A cross-sectional comparative study carried out in Nuclear Medical Centre from Aug 2009 to Jan 2010 at Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan. Patients and Methods: A total of eighty three subjects were enrolled, who were divided into three groups; group 1 comprised 31 normotensive diabetics, group 2 had 37 hypertensive diabetics while group 3 had 15 normal subjects. The DTPA GFR and creatinine clearance in healthy subjects as well as diabetic patients were compared using the unpaired student's t-test. The linear association between GFR, creatinine clearance and disease duration was expressed by Pearson's correlation coefficient 'r' along with their significance levels. Results: Gates GFR showed hyperfiltration in normotensive diabetics (96.6 +- 3.3 ml/min/1.73 m/sub 2/), significantly (p<0.05) higher than controls (85.5 +- 5 ml/min/1.73 m/sub 2/), whereas hypertensive diabetics had a significantly lower (p<0.05) Gates GFR (76.8 +- 3.7) than that of controls. Significant degree of correlation existed between GGFR and CRCL in hypertensive diabetics (p<0.05, r=0.716) and controls (r=0.546). Gates GFR also showed good correlation with duration of diabetes in both diabetic groups as compared to that of CRCL. GGFR also correlated well with duration of hypertension 0.37 (0.31-0.43) as compared to CRCL 0.155 (0.15-0.16) in all groups. Conclusions: The 99mTc-DTPA clearance correlates significantly with 24-hour creatinine clearance as well as with disease duration and can provide a simple and convenient index of kidney function in patients of early diabetic nephropathy. (author)

  18. The measurement of tracheo-bronchial mucociliary clearance with 99mTc-DTPA aerosol scintigraphy and its preliminary application in COPD patients

    International Nuclear Information System (INIS)

    Mucocoiliary clearance of respiratory channels is one of the important mechanisms guarding against retention of foreign particles within the lungs. Disorders of the mucociliary transport system play a major role among non-respiratory function in causing congenital and acquired bronchial disease. Thus, objective assay of the system is essential to recognizing and understanding abnormalities. In the present paper, a simple, noninvasive, and reliable in vivo method of monitoring mucociliary clearance function is reported. 18 healthy subjects and 32 COPD patients were studied with 99mTc-DTPA aerosol scintigraphy. Monitoring was performed by visual inspection (cinescintigraphy, to observe the distribution of 99mTc-DTPA particles and the movement of radiomucous 'hot bolus') and quantitative analysis (two indexes were utilized: the first is airway clearance ratio (ACR); the second is mucociliary clearance rate (MCC), i.e. speed of advances of mucous bolus, calculated reported by Zwas). Siemens 3700 SPECT, 64 X 64 byte mode, zoom 2. Sequential images (frame/60s) were obtained for 120 minutes. The deposition pattern of 99mTc-DTPA particles in normal subjects was uniform. The deposition pattern in COPD patients demonstrated in general a centrally located distribution with major retention in the proximal airways. Four abnormal mucous transport patterns were regionally observed: stasis, regurgitation, straying and spiral or zigzag transport. Statistical analysis showed there was a significant difference of ACR between healthy subjects and COPD patients at different time points (P<0.05). The MCC in healthy subjects and COPD patients was 3.89±0.92 mm/min and 1.32±0.59 mm/min respectively. The method of assaying tracheo-bronchial mucociliary clearance reported here is simple and objective. It has not only the advantage of visual inspection and quantitative analysis, but also has a potential usefulness in studying other bronchial diseases and evaluating of therapeutic

  19. Estimating GFR in children with 99mTc-DTPA renography: a comparison with single-sample 51Cr-EDTA clearance

    DEFF Research Database (Denmark)

    Gutte, Henrik; Møller, Michael L; Pfeifer, Andreas K;

    2010-01-01

    Glomerular filtration rate (GFR) measurement by (51)Cr-ethylenediaminetetraacetic acid (EDTA) and blood sampling in children is usually cumbersome for the patient, parents and laboratory technicians. We have previously developed a method accurately estimating GFR in adults. The aim of the present...... study was to evaluate the accuracy of this non-invasive method in children. We calculated GFR from (99m)Tc-diethylene triamine pentaacetic acid (DTPA) renography and compared with (51)Cr-EDTA plasma clearance of 29 children between the age of 1 month and 12 years (mean 4.7 years). The correlation...

  20. Assessment of the arterial input curve for [99mTc]-d,l-HM-PAO by rapid octanol extraction

    DEFF Research Database (Denmark)

    Andersen, A R; Friberg, H; Lassen, N A;

    1988-01-01

    The in vitro conversion of the lipophilic molecule [99mTc]-d,l-hexamethylpropyleneamine oxime [( 99mTc]-d,l-HM-PAO) to a hydrophilic form was studied in saline, plasma, and blood at 37 degrees C by paper chromatography and by octanol extraction. The octanol:saline ratio was 79.9. From this value...

  1. Clearance of inhaled technetium-99m-DTPA as a clinical index of pulmonary vascular disease in systemic sclerosis

    Energy Technology Data Exchange (ETDEWEB)

    Kon, O.M.; Daniil, Z.; Bois, R.M. du [Royal Brompton Hospital, Interstitial Lung Disease Unit, London (United Kingdom); Black, C.M. [Royal Free Hospital, Dept. of Rheumatology, London (United Kingdom)

    1999-01-01

    This study evaluated the utility of the clearance time of inhaled diethylenetriamine pentaacetate (DTPA) to distinguish pulmonary vascular disease from early fibrosing alveolitis (FA) in patients with systemic sclerosis (SSc) It was hypothesized that this would be preserved in patients with vascular disease compared with FA, despite similar gas-transfer deficits and matching lung volumes, because of the preservation of alveolar epithelial integrity. All patients had SSc and were categorized into a control group (C; n=9), pulmonary vascular group (VAS; n=14) or FA group (n=14) dependent on the appearance on a computed tomography (CT) scan and the transfer factor of the lung for carbon monoxide (TL,CO) (VAS and FA {<=}70%, C {>=}80%). All patients had a forced vital capacity (FVC) of >80%. The TL,CO (median) was similar in the VAS (57.5%) and FA (60%) groups. There was a significant difference in median DTPA clearance half-times between FA (21.25 min) and VAS (46.5 min) (p=0.014) and between FA and C (84.5 min) (p=0.0004). No difference was found between VAS and C (p=0.0778). Follow-up data from the VAS group showed no subsequent development of FA on the CT scan and no decrease in FVC (n=13, mean 42 months). These results suggest that clearance of diethylenetriamine pentaacetate is preserved in patients likely to have pulmonary vascular disease and may be useful in distinguishing fibrosing alveolitis from vascular disease in systemic sclerosis. (au) 22 refs.

  2. Clearance of inhaled technetium-99m-DTPA as a clinical index of pulmonary vascular disease in systemic sclerosis

    International Nuclear Information System (INIS)

    This study evaluated the utility of the clearance time of inhaled diethylenetriamine pentaacetate (DTPA) to distinguish pulmonary vascular disease from early fibrosing alveolitis (FA) in patients with systemic sclerosis (SSc) It was hypothesized that this would be preserved in patients with vascular disease compared with FA, despite similar gas-transfer deficits and matching lung volumes, because of the preservation of alveolar epithelial integrity. All patients had SSc and were categorized into a control group (C; n=9), pulmonary vascular group (VAS; n=14) or FA group (n=14) dependent on the appearance on a computed tomography (CT) scan and the transfer factor of the lung for carbon monoxide (TL,CO) (VAS and FA ≤70%, C ≥80%). All patients had a forced vital capacity (FVC) of >80%. The TL,CO (median) was similar in the VAS (57.5%) and FA (60%) groups. There was a significant difference in median DTPA clearance half-times between FA (21.25 min) and VAS (46.5 min) (p=0.014) and between FA and C (84.5 min) (p=0.0004). No difference was found between VAS and C (p=0.0778). Follow-up data from the VAS group showed no subsequent development of FA on the CT scan and no decrease in FVC (n=13, mean 42 months). These results suggest that clearance of diethylenetriamine pentaacetate is preserved in patients likely to have pulmonary vascular disease and may be useful in distinguishing fibrosing alveolitis from vascular disease in systemic sclerosis. (au)

  3. Comparison of the Glomerular Filtration Rate Estimated by Equation with That Assayed with Isotope 99m Tc-DTPA Clearance%核素99mTc-DTPA测定GFR与公式估算GFR的相关性研究

    Institute of Scientific and Technical Information of China (English)

    俞传琪; 蒋更如

    2008-01-01

    目的:探讨核素99mTc-DTPA测定GFR与MDRD公式、简化MDRD公式估算GFR的相关性.方法:178例CKD患者,用核素99mTc-DTPA测定GFR,同时测定血清肌酐、尿素氮和白蛋白,根据年龄和性别分别用MDRD和简化MDRD公式估算GFR.结果:核素99mTc-DTPA测定的GFR与MDRD公式估算的GFR有显著性差异,P=0.0001, MDRD公式高估了GFR;核素99mTc-DTPA测定的GFR与简化MDRD公式估算的GFR亦有显著性差异,P=0.0001,简化MDRD公式低估了GFR; MDRD公式估算的GFR与核素99mTc-DTPA测定的GFR呈正相关,r=0.8292, P<0.01;简化MDRD公式估算的GFR与核素99mTc-DTPA测定的GFR值呈正相关,r=0.8277, P<0.01.结论:MDRD公式、简化MDRD公式估算的GFR与核素99mTc-DTPA测得的GFR有差异,需进一步校正后用于估算GFR.

  4. Assessment of calibration curve in visual analysis by a scoring system using 99mTc-GSA liver scintigraphy

    International Nuclear Information System (INIS)

    With a view to improving the correlation of liver scintigraphy using 99mTc-DTPA-galactosyl human serum albumin (GSA) with hepatic function as indicated by indices such as LHL15, HH15, and laboratory data, the present study was carried out to set up a calibration curve (CC) for visual analysis. The application of 7 patterns of CC with different settings to a 1-min image (5 to 6 minutes after radiotracer injection) in 98 cases led to the preparation of 686 images whose correlation with hepatic function was examined by 15 observers via the grade classification of a five-point scoring system using visual analysis. The grade classification was as follows: grade 1 (faint cardiac blood-pool image), grade 2 (slight cardiac blood-pool image compared with liver uptake), grade 3 (almost the same appearance of cardiac blood-pool image and that of liver uptake), grade 4 (intense cardiac blood-pool image associated with possible liver uptake), and grade 5 (very slight liver uptake). The five-point scoring system could be used for CC slopes of 4.21, 4.79 and 2.79 (the above correlation was strongest for 4.21). The significance of inter-grade group differences in ICG-R15, LHL15 and heart/liver ratio was examined according to slope. With the CC slope of 4.21, a significant inter-grade group difference was observed between grades 1 and 2, grades 2 and 3, grades 3 and 4, and grades 4 and 5. In conclusion, when the value of the CC slope was 4.2, it was considered appropriate for grade classification on a five-point scoring system using visual analysis. (author)

  5. Study with radio aerosol of DTPA technetium-99 m in individuals with pulmonary disease by amiodarone

    International Nuclear Information System (INIS)

    In order to evaluate the role of the clearance of 99 m Technetium chelated to diethylenetriamine-penta-acetate (99 m Tc-DTPA) in amiodarone induced pulmonary disease, 40 individuals were studied in four groups. After spirometry, where a volume-time curve was registered, all individuals inhaled 740 MBq of 99 m Tc-DTPA diluted in 4 ml of saline, for five minutes. Pulmonary images were obtained in a computerized scintillation camera and 9 regions of interest were selected. (author)

  6. Effect of background region of interest and time-interval selection on glomerular filtration ratio estimation by percentage dose uptake of (99m)Tc-DTPA in comparison with (51)Cr-EDTA clearance in healthy cats.

    Science.gov (United States)

    Debruyn, Katrien; Vandermeulen, Eva; Saunders, Jimmy H; Dobbeleir, André A; Ham, Hamphrey R; Peremans, Kathelijne

    2013-08-01

    Evaluation of glomerular function is a useful part of the diagnostic approach in animals suspected of having renal disease. Time-interval and background region of interest (bg ROI) selection are determining factors when calculating the glomerular filtration ratio (GFR) based on percentage uptake of (99m)technetium-labelled diethylene triamine penta-acetic acid ((99m)Tc-DTPA). Therefore, three different time intervals (60-120 s, 120-180 s, 60-180 s) and three different bg ROIs (C-shape, caudolateral, cranial + caudal) were investigated. In addition, global GFRs based on percentage dose uptake of (99m)Tc-DTPA for the different time-intervals and bg ROIs were compared with the global GFR based on (51)chromium-ethylene diaminic tetra-acetic acid ((51)Cr-EDTA) plasma clearance in nine healthy European domestic shorthair cats. Paired Student's t-tests and linear regression analysis were used to analyse the data. Different time intervals seemed to cause significant variation (P <0.01) in absolute GFR values, regardless of the choice of bg ROI. Significant differences (P <0.01) between bg ROIs were only observed in the 120-180s time interval between the C-shape and cranial + caudal bg ROI, and between the caudolateral and cranial + caudal bg ROI. The caudolateral bg ROI in the 60-180 s time interval showed the highest correlation coefficient (r = 0.882) between (99m)Tc-DTPA and (51)Cr-EDTA, although a significant difference (P <0.05) was present between both techniques. PMID:23349527

  7. Inhalation scintiscanning of persons with healthy lungs and lung diseases using sup(99m)Tc-sulfur colloid with special regard to the mucociliary clearance

    International Nuclear Information System (INIS)

    It is reported about inhalation scintiscanning with radioaerosols under the application of a gamma camera and a magnetic core- and tape memory, providing an interactive display. 89 patients suffering form various pulmonary diseases and 10 test persons with healthy lungs were investigated. The scintigraphic photos were not only evaluated visually, but also according to quantitative parameters, i.e. left/right distribution, degree of central deposition and mucociliary clearance. The latter resulted to be the most informative quantitative diagnostic method. In the 10 test persons with healthy lungs the scintigraphic images always showed a homogeneous distribution of radioactivity and a mucociliary half-time of 9.7 or 11.9 hours respectively was found. A central bronchial carcinoma provoked on the affected side of the lungs a reduced deposition of radioactivity, an increased central deposition and an accelerated mucociliary clearance. In patients suffering from peripheral bronchial carcinoma, pneumonia, pleural processes, pulmonary infarction, pulmonary circular focus or pneumothorax, only inconstant areas of reduced radioactivity deposition could be detected. However, these areas differed by shape, intensity and location from the central bronchial carcinoma. In 3 patients with pulmonary embolism, no pathologic changes could be detected only then, when no infarction occurred. In this case, also the quantitative parameters were normal. With chronical obstructive pulmonary diseases, diseases of the pulmonary framework and in cases of bronchial asthma a clearly pathologic image of the distribution of radioactivity resulted in all cases; in these a differentiation could only be achieved by additionally considering the quantitative parameters. (orig./MG)

  8. Labeling of creatinine with technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Yurt Lambrecht, F. [Ege Univ., Bornova, Izmir (Turkey). Dept. of Nuclear Applications, Inst. of Nuclear Sciences; Durkan, K. [Dokuz Eylul Univ., Buca, Izmir (Turkey). Chemistry Technicianship Program, Izmir Vocational School; Soylu, A. [Dokuz Eylul Univ., Narlidere, Izmir (Turkey). Dept. of Pediatrics, Medical Faculty

    2004-07-01

    Creatinine is a clinically important index of renal glomerular filtration rate. Urine creatinine levels can be used as a screening test to evaluate kidney function or can be part of the creatinine clearance test. In case of kidney dysfunction or muscle disorders the creatinine concentration in serum/plasma may rise to a higher value than in healthy body. Technetium- 99m has been used in nuclear medicine and in biomedical research to label molecular and cellular structures employed as radiotracers. {sup 99m}Tc is utilized to label molecules and cells, used as radiopharmaceuticals, and also to label biological species. It presents many desirable characteristics. SnCl{sub 2} method is frequently used as a reducing agent in the {sup 99m}Tc- labeling process. Creatinine metabolism might be investigated by using labeled {sup 99m}Tc- creatinine in healthy or uremic rats. (orig.)

  9. Evaluation of lung epithelial permeability in the volatile substance abuse using Tc-99m DTPA aerosol scintigraphy

    International Nuclear Information System (INIS)

    Chronic inhalant use is associated with significant toxic effects, including neurological, renal, hepatic, and pulmonary damage. However, there is a paucity of reports regarding respiratory complications in inhalant abusers. The aim of this study was to evaluate pulmonary epithelial permeability in the volatile substance abuse (VSA) using technetium-99m-labeled diethylenetriamine pentaacetic acid (Tc-99m DTPA) aerosol scintigraphy. This study included 18 patients with volatile substance abuse and 18 volunteer controls. All of patients and controls were smokers. Tc-99m DTPA aerosol scintigraphy was performed in all cases. Time-activity curves from each lung were generated and clearance half-time (T1/2) of Tc-99m DTPA were calculated. T1/2 of whole lung was calculated as a mean of the T1/2 of left and right lung. The T1/2 values of Tc-99m DTPA clearance in the substance abusers were significantly decreased as compared to the control group with respective mean values of 28.86±8.44, and 62.14±26.12 min (p=0.001). It was seen Tc-99m DTPA clearance from lung was faster as the duration of substance abuse was increased. Tc-99m DTPA pulmonary clearance is markedly accelerated in the volatile substance abuse. This suggests that inhalant abuse of substance may produce abnormalities in pulmonary alveolo-capillary membrane function. (author)

  10. Detection of alveolar epithelial injury by Tc-99m DTPA radioaerosol inhalation lung scan in rheumatoid arthritis patients

    International Nuclear Information System (INIS)

    Rheumatoid arthritis (RA) is a systemic autoimmune disorder primarily involving the joints. Lung alterations in RA may be primary or secondary to pharmacological treatments and may involve the alveoli, interstitium, airways and/or pleura. Technetium-99 m diethylenetriaminepentaacetic acid (Tc-99m DTPA) aerosol inhalation scintigraphy is a sensitive and noninvasive test commonly employed to assess pulmonary epithelial membrane permeability. The purpose of this study was to investigate the changes of pulmonary alveolar epithelial permeability in patients with RA, to determine the relationship between the clearance rate of Tc-99m DTPA and pulmonary function test (PFT) results, and to determine the relationship between the clearance rate of Tc-99m DTPA and clinical parameters of disease. Twenty-five patients with RA but without lung alterations were included in the study. The patients were 22 females, and 3 males; mean age 53.6±8.7 years. Technetium-99m DTPA aerosol inhalation scintigraphy was performed on the study and healthy control groups. Clearance half times (T1/2) were calculated by placing a mono-exponential fit on the curves. Penetration index (PI) was calculated on the first-minute image. There were no significant differences in the mean T1/2 or mean PI values between the RA patients and control subjects. No correlation was found between the mean T1/2 values of Tc-99m DTPA clearance and activity of RA, clinical values, or the spirometric measurements except FEV1/FVC and functional status in RA patients (p=0.02, p=0.01, respectively). However, a weak correlation was found between duration of disease and T1/2 values of Tc-99m DTPA clearance (p=0.006). PI values tended to correlate with FEF25-75, although, this was not statistically significant (p=0.057). This study shows that no changes occur in alveolar-capillary permeability in RA patients without lung alterations. (author)

  11. 三种肾小球滤过率检测方法与99mTc-DTPA清除率的比较与分析%Evaluation on three kinds of glomerular filtration rate and comparison with 99mTc-DTPA clearance

    Institute of Scientific and Technical Information of China (English)

    唐琦; 史浩; 王伟铭; 陈楠; 陈晓农; 徐耀文

    2005-01-01

    目的为临床准确评估肾小球滤过率(GFR)提供一定依据.方法对101例各种慢性肾脏病患者,外周静脉注射非离子型造影剂碘海醇(iohexol)5 ml,4 h后取血2ml,用高效液相色谱(HPLC)法检测血浆iohexol浓度,运用数字模型计算血浆iohexol清除率(iohexol-GFR).同步检测Scr及99mTc-DTPA清除率(99mTc-GFR).应用Cockcroft-Gault(CG)、肾脏病膳食改良试验(MDRD)公式计算Ccr、GFR.以99mTc-GFR为标准,运用相关回归分析及受试者工作曲线(ROC)对上述指标进行比较.结果慢性肾脏病第1期至第5期,iohexol-GFR与99mT-GFR相关系数r分别为0.87、0.89、0.88、0.86、0.87(P均<0.01);iohexol-GFR在ROC曲线下面积平均为0.97.MDRD-GFR与99mTc-GFR相关系数r分别为0.80、0.75、0.71、0.67、0.56(P均<0.01),MDRD-GFR在ROC曲线下面积平均为0.82.CG-Ccr与99mTc-GFR相关系数r分别为0.76、0.67、0.62、0.60、0.53(P均<0.05),CG-Ccr在ROC曲线下面积平均为0.82.结论iohexol-GFR检测GFR的准确性优于MDRD-GFR、CG-Ccr,值得推广应用.在无检测条件的情况下,CG、MDRD公式不失为衡量GFR的良好指标.

  12. Tc-99m imaging agents

    International Nuclear Information System (INIS)

    A wide range of pharmaceuticals for labeling with Tc-99m, developed by the Soreq Radiopharmaceuticals Department, is described. Details of the production and quality control of 13 kits are given, as well as the range of results required for consistently high quality imaging agents

  13. Uptake of {sup 99m}Tc tetrofosmin in lymphoma cell lines: a comparative study with {sup 99m}Tc sestamibi

    Energy Technology Data Exchange (ETDEWEB)

    Ding, H.-J.; Shiau, Y.-C.; Tsai, S.-C.; Wang, J.-J.; Ho, S.-T.; Kao, Albert E-mail: albertkaotw@yahoo.com.tw

    2002-06-01

    Technetium-99m ({sup 99m}Tc) tetrofosmin has been used as a tumor-seeking agent. However, its role in detecting lymphomas has not been widely investigated. The aim of the present study was to determine the uptake and clearance characteristics of {sup 99m}Tc tetrofosmin in lymphoma cell lines. {sup 99m}Tc sestamibi was also evaluated for comparison. Three lymphoma cell lines (U-937: monocyte-like, histiocytic lymphoma, human; RAMOS: B-lymphoma cell line, American Burkitt lymphoma, lymphoblastoid, human; Hs445: Hodgkin's disease, lymphoid, human) were studied. After incubation of radiotracers {sup 99m}Tc tetrofosmin and {sup 99m}Tc sestamibi in medium for 0, 10, 20, 30, 60, 120 and 180 min, the uptake and clearance of each radiotracer were measured in the three lymphoma cell lines. The uptake of {sup 99m}Tc tetrofosmin was lower than that of {sup 99m}Tc sestamibi in these lymphoma cell lines. Among the three cell lines, Hs445 showed the greatest {sup 99m}Tc tetrofosmin uptake capacity. RAMOS and U-937 showed similar {sup 99m}Tc tetrofosmin uptake capacities. {sup 99m}Tc tetrofosmin accumulated in the three tested lymphoma cell lines, especially in the Hodgkin's disease cell line. However, in comparison with {sup 99m}Tc sestamibi, {sup 99m}Tc tetrofosmin may not be the best radiotracer for detection of lymphoma.

  14. Assessment of alveolar epithelial permeability in Behcet's disease with 99mTc-DTPA aerosol scintigraphy

    International Nuclear Information System (INIS)

    Behcet's disease (BD) is a multisystem disorder characterized by vasculitis, and consists of a triad of recurrent ulcers of the oral and genital mucosa with relapsing uveitis. The prevalance of pulmonary involvement varies in the range of 1-10% in various studies and its complications are severe and life threatening. In this study, we investigated the changes of pulmonary epithelial permeability of patients with BD using technetium-99m diethylene triamine penta-acetic acid (99mTc-DTPA) aerosol scintigraphy, so as to begin the therapy regimen as soon as possible. Twenty-one nonsmoking patients with BD (8 women, 13 men; mean age 38.67±8.86 years) and 15 healthy volunteer nonsmoking controls (8 women, 7 men; mean age 50.87±12.45 years) underwent 99mTc-DTPA aerosol inhalation scintigraphy and pulmonary function tests (PFTs). Subjects inhaled 1480 MBq of 99mTc-DTPA for 4 min in the supine position. Scintigraphic data were recorded dynamically (1 frame/min) in the posterior projection on a 64 x 64 matrix for a 30-min period using a double-headed gamma camera (Infinia, GE, Tirat Hacarmel, Israel) equipped with a low-energy all-purpose parallel hole collimator. Half time of 99mTc-DTPA clearance (T1/2) was calculated by placing a mono-exponential fit on the curves. Penetration index (PI) was also calculated by dividing the peripheral total counts by the sum of the peripheral and central total counts on the first minute image, in order to quantify the distribution of the inhaled aerosol. The clearance half time of 99mTc-DTPA radioaerosols in the BD patients (24.81±6.22 min) was faster than in the normal control group (46.53±22.41 min) (P=0.004). There was also a significant difference between PI of the patients with BD (0.15±0.03) and that of the controls (0.21±0.06) (P=0.002). No correlation was found between the mean T1/2 values of 99mTc-DTPA clearance or the spirometric measurements in the BD patients. Penetration indices were not correlated with PET in the BD

  15. Urinary bladder blood flow. I. Comparison of clearance of locally injected 99mtechnetium pertechnate and radioactive microsphere technique in dogs

    DEFF Research Database (Denmark)

    Krøyer, Kristian; Bülow, J; Nielsen, S L;

    1990-01-01

    The blood flow of the dog urinary bladder measured by radioactive microsphere technique was compared to the clearance of locally injected 99mTechnetium pertechnate (99mTc) in the bladder wall. In semilogarithmic plots the 99mTc washout curves showed a multiexponential course. From the initial slo...

  16. Scintigraphic and intraoperative scintimetric localization of an ectopic parathyroid adenoma with Tc-99m Tetrofosmin and Tc-99m Sestamibi

    International Nuclear Information System (INIS)

    Background: We report on a case of preoperative scintigraphic and intraoperative scintimetric confirmation of an ectopic parathyroid adenoma with Tc-99m Tetrofosmin in a 52-year old patient with persistent hyperparathyroidism after unsuccessful primary preoperative localization and reoperation. Methods: In preoperative localization study, dynamic, sequential static scans and SPECT of the neck and mediastinum were performed using Tc-99m Sestamibi and Tc-99m Tetrofosmin. Reoperation was performed by help of a hand-held gamma-probe for intraoperative scintimetric localization after preoperative injection of Tc-99m Tetrofosmin. Results: An ectopic adenoma could be detected preoperatively with both tracers. Tc-99m Tetrofosmin showed no significant difference in image quality vs. Tc-99m Sestamibi. The adenoma could also be detected scintimetrically by elevated count-rates. Conclusions: This case gives promise for Tc-99m Tetrofosmin to be a possible alternative in parathyroid imaging with similar imaging properties and slight theoretical advantages concerning background clearance, radiation exposure and constitution over Tc-99m Sestamibi. Intraoperative localization and confirmation of ectopic parathyroid adenoma, eventually with a geometrically adapted gamma-probe, seems to be possible. Whether there are significant advantages resulting in a possible reduction of operating time, should be evaluated in further studies. (author)

  17. QC studies and biological evaluations of three locally produced renal pharmaceutical preparations: 99mTc-DTPA, 99mTc-GH, and 99mTc- DMSA

    International Nuclear Information System (INIS)

    Quality control (QC) studies were done of three locally produced renal preparations of 99mTc- DTPA, 99mTc-GH and 99mTc-DMSA, as part of the production activity before the Gulf war. These studies were performed using gel chromatography column scanning (GCS) technique, although there are other alternative techniques. These kits were tested for radiochemical purity, and the labeling yields were found to be excellent. Biological studies included the following parameters: organ distribution (in mice, rats and rabbits), blood clearance in rabbits, and plasma protein binding in rats at different time intervals. Poor renal concentration of three agents in mice was significant at two time intervals. The tissue distribution in mice of DTPA, GH, and DMSA kits, which were stored at 37 deg. C for 30 days, after intravenous injection of the radioactivity at 5, 30, and 60 minutes, respectively, is presented. Blood retention of the renal agents was considerably low compared to those in rats and rabbits at the respective time intervals. The blood clearance of 99mTc-DMSA was relatively slow, and 99mTc-GH has rapid blood clearance, identical with that of 99mTc-DTPA for the first 20 minutes but slower thereafter. The binding of DTPA, GH and DMSA with plasma protein was 5, 65, 95 % at 1 h, respectively. Various amounts of tin (II) (as SnCl2), were used in the kits, with up to 10 times more of the usual dose for respective tin-complex. It was observed that there was no significant variation of the organ distribution in mice

  18. 计算法与应用99mTc-DTPA清除率测定肾小球滤过率的临床对比研究%The clinical contrast study of determining glomerular filtration rate by calculation and using clearance rate of99mTc-DTPA

    Institute of Scientific and Technical Information of China (English)

    吴锡信; 陈江林; 彭健

    2002-01-01

    目的评价临床应用计算法测定肾小球滤过率(GFR)的准确性.方法用99mTc-DTPA清除率测定60例不同疾病住院病人GFR(Tc-GFR),并测血清肌酐,同时以WCP公式测定GFR(WCP-GFR),以Cockcroft/Gault公式计算内生肌酐清除率(CG-CCr).肾功能不全及正常组WCP-GFR、CG-GFR分别与Tc-GFR进行相关分析,比较两组WCP-GFR、CG-GFR与Tc-GFR间显著性差异以及WCP-GFR、Tc-GFR间平均差和CG-GFR、Tc-GFR间平均差的显著性差异.结果 WCP-GFR及CG-CCr均可在一定程度上准确反映GFR,而以WCP-GFR数值更接近Tc-GFR,准确性更高,临床上可代替Tc-GFR.结论 WCP-GFR是一种准确、简便、快捷测定GFR方法.

  19. In vivo hepatic uptake inhibition study of Tc-99m-labeled hepatobiliary agents

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Meyoung Kon [National Institutes of Health, Bethesda (United States)

    1998-07-01

    We have previously reported that the hepatic uptake of Tc-99m-mercaptoacetyltriglycine (MAG3)-biocytin was not affected by coinjecting bilirubin in mice whereas the uptake of Tc-99m-diisopropyliminodiacetic acid (DISIDA) was inhibited (Kim et al. J Nucl Med, 38 : 50 p, 1997). The aim of this study was to investigate whether the hepatic uptake of Tc-99m-MAG3-biocytin or Tc-99m-DISIDA could be inhibited by biotin and biocytin. The Balb/c mice (female, 20g, n=5-8) were injected i.v. with the hepatobiliary agents alone (15-22 MBq/20-40 {mu}g) or together with inhibitors at two doses (14 and 28 mg/kg). For pharmacokinetic studies, images were acquired at 10-sec intervals for 20 min using a gamma camera equipped with a pin-hole collimator (d= 1 mm), starting immediately after intravenous injection. Pharmacokinetic parameters, peak liver/heart ratio (Rmax) and hepatic half clearance time (HCT), were calculated from liver and heart time-acitivity curves from regions-of-interest. Dynamic images showed rapid hepatic uptake inhibition was characterized by persistent high blood background. These qualitative scintigraphic findings were reflected in the pharmacokinetic parameters. Tc-99m-MAG3-biocytin without inhibitor coinjection showed Rmax of 9.3 and HCT of 383 sec. These parameters did not change significantly when bilirubin or biotin was coinjected, but did change significantly (P<0.05) for biocytin only at the higher dose: 52% decrease in HCT. In contrast, the parameters for Tc-99m-DISIDA (Rmax of 9.2 and HCT of 258 sec) were greatly affected (P<0.01) by biotin (79% decrease in Rman and 2-fold increase in HCT) even at 14 mg/kg concentration. We concluded that Tc-99m-MAG3-biocytin is less sensitive to inhibition by bilirubin, biotin, and biocytin than Tc-99m-DISIDA. Tc-99m-MAG3-biocytin appears to be a promising hepatobiliary imaging agent for hepatic function studies and may also be a useful tool to investigate the hepatic uptake mechanism of biotin derivatives in vivo.

  20. Spiperone dithiocarbamate- 99mTc kit - a potential diagnosis agent for dopaminergic D-2 brain pathologies - biodistribution

    International Nuclear Information System (INIS)

    Psycho pharmacology has been discovering much about the D 2 dopamine receptors and their interrelationship to brain pathologies such as Parkinson's Disease, Schizophrenia and Huntington Disease. Those biological receptors have got affinity with dopamine endogenous agent, so that they complex and, in non pathological individuals, the biological receptors contribute to bring the levels of dopamine and free acetylcholine into equilibrium. The Spiperon Dithiocarbamate (SPDC) from Spiperon is synthesized and its complexation with Technetium-99 m has been prepared with its reaction parameters after being studied and improved. The SPDC- 99m Tc complex biological distribution has been made in Wistar rats and the uptake of spleen, heart, liver, stomach, lung, kidney, blood, intestine and brain have been resolved. The plasmatic clearance curve has been based on Wistar rats data and the Know-how of the kit ( for label SPDC with 99m Tc) has been achieved. (author)

  1. Radiopharmacokinetic data for 99mTc-ABP - A new radiopharmaceutical for bone scanning: Comparison with 99mTc-MDP

    International Nuclear Information System (INIS)

    Technetium-99m-labeled alendronate is a new radiopharmaceutical for bone scanning developed under strict quality control at the INNSZ. The purpose of this work was to compare the radiopharmacokinetic data and the dosimetry of 99mTc-ABP and 99mTc-MDP in 10 volunteers, after it was tested in laboratory animals. 99mTc-ABP has shorter mean residence time (MRT) and t(1(2)) β; is less protein bound; has a higher renal clearance; smaller Vdss, and similar bone uptake at 1 and 2 h. 99mTc-ABP gives less radiation exposure to the patient with a 740 MBq dose, and the quality of the bone scan is excellent. 99mTc-ABP is a better radiopharmaceutical than 99mTc-MDP for bone scanning

  2. Technetium-99m-diethyl-IDA instant kit

    International Nuclear Information System (INIS)

    A formulation of stannous-diethyl-IDA freeze-dried kit, containing 50 mg diethyl-IDA and 0.4 mg hydrated stannous chloride, to be labelled with technetium was developed for hepatobiliary scintigraphy. The organ distribution data of 99mTc-diethyl-IDA in mice for 60 min post injection were satisfactory. The radiopharmaceutical exhibits rapid blood clearance, great hepatic clearance and very short hepatocyte transit time. Uptake of the radiopharmaceutical was highest in mouse liver and intestine. The renal uptake of the HB agent in mice is relatively low. Blood clearance data showed that the HB agent is rapidly cleared. (author) 20 refs.; 4 tabs

  3. The placental binding and transfer of radiopharmaceuticals II: Tc-99m Sestamibi, Tc-99m Tetrofosmin and Tl-201 Thallous Chloride

    International Nuclear Information System (INIS)

    It has been shown that some radiopharmaceuticals can cross the placental barrier and enter into fetal circulation. The objective of this study was to investigate placental transfer of cardiac perfusion imaging radiopharmaceuticals (Tc-99m sestamibi, Tc-99m tetrofosmin and TI-201 thallous chloride (Tl+ -201)) in pregnant guinea pigs and in-vitro mechanism of the transfer using human placental lobule. Pregnant guinea pigs in the first, second and third trimesters were used. Following intravenous injection of Tc-99m sestamibi, Tc-99m tetrofosmin or Tl+ -201 the guinea pig was imaged using a gamma camera; killed and the fetuses removed. Each fetus was then imaged separately using a low energy general-purpose collimator. The placentas, ovary, and fetal hearts, lungs, liver, kidneys and blood were then removed and the radio-activity in each organ along with a 1.0 ml standard solution was counted separately in a dose calibrator. For the in-vitro experiment, the effect of ouabain on the transfer of Tl+ -201 and tritiated water (as internal reference marker) in the maternal-fetal direction was studied in human placentas (collected postpartum) from normal uncomplicated pregnancies and suitable lobules perfused with either normal or ouabain-treated perfusate. The differential transport rate of Tl+ -201 in the maternal-fetal direction was measured as time taken in minutes for 50% fraction of Tl+ -201 (TR50 ) to be transported across the fetal vein and expressed as the ratio of the TR50 of Tl+ -201 to tritiated water (TR50 index). The following pharmacokinetic parameters were also measured: the area under the curve, clearance and absorption rate indices. Neither Tc-99m sestamibi nor Tc-99m tetrofosmin was transferred across the placental barrier while 1.7 ± 0.5% of the injected activity (0.027 ± 0.005 % per gram) of Tl+ -201 radioactivity during the third trimester was transferred across the placenta into fetal circulation. Most of the radioactivity from Tl+ -201 in the fetus

  4. Diagnosis of pulmonary infections with HIV (+) patients. Brought of aerosol DTPA-Tc99m and of Ga67 citrate

    International Nuclear Information System (INIS)

    The pulmonary clearance of aerosol DTPA-Tc99m is a technology easy to use, well support by patients with immediate results. With the pneumonia at Pneumocystis Carinii (PPC), the clearance is more sensitive and more specific than the thoracic scintigraphy with Ga67. Used with a thorax radiography, results will lead to three directions: High probability of PPC, di phasic curve and very fast T50, equal inferior to 5,10 mn. Low probability of PPC, monoexponential curve and abnormal thorax radiography or di phasic curve with a value T50 superior to 5,10 mn. With these patients other explorations will be made (Ga67, biopsy) and if possible search pulmonary tuberculosis. Extra pulmonary pathology: monoexponential curve associated with a normal thoracic radiography. 2 figs

  5. Diagnostic efficiency of sup(99m)Tc-phytic colloid in the studies of hepatosplenic area

    International Nuclear Information System (INIS)

    High diagnostic efficiency of sup(99m)Tc-phytic frostimag colloid for studies of the anatomic-topographic characteristics of the liver and spleen, and its advantages over sup(99m)Tc-sulphide colloid for studies of splenic clearance have been shown. The profile scanner (MNIRRi-GAMMA) was used for the quality control over the preparation of Tc-labelled colloids

  6. Cyclotron Production of Technetium-99m

    Science.gov (United States)

    Gagnon, Katherine M.

    Technetium-99m (99mTc) has emerged as the most widely used radionuclide in medicine and is currently obtained from a 99Mo/ 99mTc generator system. At present, there are only a handful of ageing reactors worldwide capable of producing large quantities of the parent isotope, 99Mo, and owing to the ever growing shutdown periods for maintenance and repair of these ageing reactors, the reliable supply 99mTc has been compromised in recent years. With an interest in alternative strategies for producing this key medical isotope, this thesis focuses on several technical challenges related to the direct cyclotron production of 99mTc via the 100Mo(p,2n)99mTc reaction. In addition to evaluating the 100Mo(p,2n)99mTc and 100Mo(p,x)99Mo reactions, this work presented the first experimental evaluation of the 100Mo(p,2n) 99gTc excitation function in the range of 8-18 MeV. Thick target calculations suggested that large quantities of cyclotron-produced 99mTc may be possible. For example, a 6 hr irradiation at 500 μA with an energy window of 18→10 MeV is expected to yield 1.15 TBq of 99mTc. The level of coproduced 99gTc contaminant was found to be on par with the current 99Mo/99mTc generator standard eluted with a 24 hr frequency. Highly enriched 100Mo was required as the target material for 99mTc production and a process for recycling of this expensive material is presented. An 87% recovery yield is reported, including metallic target preparation, irradiation, 99mTc extraction, molybdate isolation, and finally hydrogen reduction to the metal. Further improvements are expected with additional optimization experiments. A method for forming structurally stable metallic molybdenum targets has also been developed. These targets are capable of withstanding more than a kilowatt of beam power and the reliable production and extraction of Curie quantities of 99mTc has been demonstrated. With the end-goal of using the cyclotron-produced 99mTc clinically, the quality of the cyclotron

  7. Technetium-99m Sestamibi in Multiple Myeloma

    International Nuclear Information System (INIS)

    Technetium-99m 2-methoxy - isobutyl - isonitrile (99mTc-MIBI) has been reported to be useful in evaluating patients with multiple myeloma. The aim of this study is to evaluate the role of technetium-99m sestamibi (99mTc-MIBI) scintigraphy in the diagnosis. staging and follow-up of patients with multiple myeloma. Methods and Materials: twenty-five consecutive patients with multiple myeloma were studied using 99mTc- MIBI. Of the 25 patients included in this study, 6 were in stage I, II in stage II and 8 in stage III. Anterior and posterior whole-body imaging were obtained 20 min after I.V. injection of 740 MBq of 99mTc-MIBI. Four different MIBI patterns could be described in our patients: physiological (P), diffuse (D), focal (F) and combined diffuse and focal (D+F). All patients in stages II and III as well as 3 patients in stage I were treated with chemotherapy (cyclophosphamide and prednisone) then 99mTc-MlBI scans were repeated after 6 courses. Results: in comparison to conventional X-ray skeletal survey, 99mTc-MIBI scans showed a higher number of myeloma bone disease at diagnosis. All patients with stage II and III multiple myeloma were positive with 99mTc-MlBl scans at diagnosis. The pattern of positive MIBI accumulation was diffuse in 13 (52%) patients, focal in 4 (16%) and combined focal and diffuse in 6 (24%) patients. The intensity of 99mTc-MIBI correlated with disease activity as determined by lactate dehydrogenase (LDH), number of plasma cells in bone marrow and serum electrophoresis. There was a direct correlation between 99mTc-MIBI scan result and clinical outcome of patients following 6 courses of chemotherapy. Sensitivity and specificity of 99mTc-MIBI scintigraphy in detecting myeloma bone lesions were 92% and 90% respectively. Conclusion: 99mTc-MIBI scintigraphy is a reliable method to evaluate bone marrow activity in patients with multiple myeloma and follow-up of myeloma bone lesions

  8. Combined Tc-99m sesta MIBI scin

    Directory of Open Access Journals (Sweden)

    Enas Abdel Ghany Ibrahim

    2015-12-01

    Conclusions: Accurate localization of adenomas should be obtained prior to surgery for minimal invasive surgical procedure. In this study, combined ultrasonography and Tc-99m MIBI improved localization of parathyroid adenomas.

  9. Comparative scanning of thyroid nodules with technetium-99m pertechnetate and technetium-99m methoxyisobutylisonitrile

    Energy Technology Data Exchange (ETDEWEB)

    Foeldes, I.; Levay, A. (Central Hospital of the Ministry of the Interior, Budapest (Hungary). Dept. of Nuclear Medicine); Stotz, G. (Central Hospital of the Ministry of the Interior, Budapest (Hungary). Dept. of Pathology)

    1993-04-01

    Thyroid imaging was performed using technetium-99m methoxyisobutylisonitrile and technetium-99m pertechnetate in 58 patients. The [sup 99m]Tc-pertechnetate scans showed a total of 77 nodules: 60 cold, 13 hot and 4 of normal acitivity. There was no [sup 99m]Tc-MIBI accumulation in 46.4% of [sup 99m]TC-pertechnetate cold nodules; 27 (45%) of these nodules showed [sup 99m]TC-MIBI uptake with the same intensity as the surrounding normal tissue, and five (8.6%) became hot with [sup 99m]Tc-MIBI. Of the [sup 99m]TC-pertechnetate hot nodules 11 (84.6%) could not be differentiated from the normal extranodular tissue on the [sup 99m]Tc-MIBI scan. The histopathology of 34 surgically removed nodules proved that increased, normal or decreased [sup 99m]Tc-MIBI accumulation is not specific for thyroid malignance and that the [sup 99m]Tc-MIBI uptake depends mainly on the viability of thyroid tissue. (orig.).

  10. Detection of alveolar epithelial injury by 99mTc-DTPA radioaerosol inhalation lung scan following blunt chest trauma

    International Nuclear Information System (INIS)

    DTPA clearance rate is a reliable index of alveolar epithelial permeability, and is a highly sensitive marker of pulmonary epithelial damage, even of mild degree. In this study, 99mTc-DTPA aerosol inhalation scintigraphy was used to assess the pulmonary epithelial membrane permeability and to investigate the possible application of this permeability value as an indicator of early alveolar or interstitial changes in patients with blunt chest trauma. A total of 26 patients was chest trauma (4 female, 22 male, 31-80 yrs, mean age; 53±13 yrs) who were referred to the emergency department in our hospital participated in this study. Technetium-99m diethylene triamine pentaacetic acid (DTPA) aerosol inhalation scintigraphy was performed on the first and thirtieth days after trauma. Clearance half times (T1/2) were calculated by placing a mono-exponential fit on the curves. Penetration index (PI) was calculated on the first-minute image. On the first day, mean T1/2 value of the whole lung was 63±19 minutes (min), and thirtieth day mean T1/2 value was 67±21 min. On the first day, mean PI values of the lung and 30th day mean PI value were 0.60±0.05, and 0.63 ±0.05, respectively. Significant changes were observed in radioaerosol clearance and penetration indices. Following chest trauma, clearance of 99mTc-DTPA increased owing to breakdown of the alveolar-capillary barrier. This increase in the epithelial permeability of the lung appears to be an early manifestation of lung disease that may lead to efficient therapy in the early phase. (author)

  11. Experimental studies on imaging of infected site with 99mTc-Iabeled ciprofloxacin in mice

    Institute of Scientific and Technical Information of China (English)

    ZHANG Hong; JIANG Ning-yi; ZHU Lin

    2009-01-01

    Background Bacterial infection can pose a substantial diagnostic dilemma.99mTc-labeled ciprofloxacin (CPF) was developed as a biologically active radiopharmaceutical to diagnose infection.In the present research,we studied the biodistribution and imaging properties of infection tracer 99mTc-CPF in a mouse model of infection.Methods CPF was labeled with 99mTc and the radiochemical purity and labeling rate were measured.A mouse model of infection was established.We then determined the biodistribution of 99mTC-CPF and conducted the whole body scintigraphy of the animal model.Results 99mTc-Ciprotech was stable for at least 6 hours at room temperature.The labeling rate of CPF by 99mTc was over 90%.Clearance of radioactivity mainly occurred in the liver and kidney,and the clearance from blood was rapid.Both biodistribution and imaging results showed higher uptake of 99mTc-CPF at sites of infection.The infectious tissue/normal tissue ratio peak was 4.30 at 4 hours after injection.Conclusions 99mTc-CPF is a sensitive radiopharmaceutical for scintigraphy of infectious lesions and it is easy to prepare.

  12. Sclerosing cholangitis: cholescintigraphy with Tc-99m-labeled DISIDA

    Energy Technology Data Exchange (ETDEWEB)

    Ament, A.E.; Bick, R.J.; Miraldi, F.D.; Haaga, J.R.; Wiedenmann, S.D.

    1984-04-01

    Four patients with primary sclerosing cholangitis (PCS) were examined with the hepatobiliary agent Tc-99m-labeled DISIDA (diisopropylphenylcarbamoyl iminodiacetic acid), and the results correlated with those of invasive cholangiography. Cholescintigraphy is superior to cholangiography in cases of suspected PSC where there is nonfilling of biliary radicals due to high-grade stenosis. The finding of delayed hepatic parenchymal clearance can allow estimation of the degree of obstruction of the various branches of the major bile ducts. Cholescintigraphy offers a noninvasive method of investigating patients with suspected sclerosing chloangitis, leading to earlier diagnosis. Confirmation with invasive cholangiographic procedures is recommended.

  13. A kinetic model for 99mTc-DMSA in the rat.

    Science.gov (United States)

    Maneval, D C; D'Argenio, D Z; Wolf, W

    1990-01-01

    A pharmacokinetic model was developed for the renal imaging agent 99mTc-DMSA in anesthetized rats, which incorporated data from serial measurements of blood and urine simultaneously with dynamic images obtained over an 8-h period. Animals which received a 10 mg/kg dose of unlabeled DMSA immediately before 99mTc-DMSA injection had a significantly reduced kidney accumulation and greater urinary elimination of 99mTc than animals which received the radiopharmaceutical alone. The kidney clearance was also significantly lower in rats receiving unlabeled DMSA, but no significant difference was determined between the urine clearance estimates of the two animal groups. Because the increase in the amount eliminated in the urine was not coupled with a significant change in urine clearance, it would appear that unlabeled DMSA saturated the kidney uptake mechanism(s) of 99mTc-DMSA without modifying the urinary clearance process. This interpretation is consistent with the hypothesis that renal handling of 99mTc-DMSA is governed by both glomerular filtration and peritubular capillary uptake. The simultaneous acquisition of blood, urine and non invasive image data allows for a comprehensive and informative model of the physiological disposition of 99mTc-DMSA.

  14. Clinical need for both scintigraphy with technetium-99m GSA and per-rectal portal scintigraphy in some patients with chronic liver disease

    Energy Technology Data Exchange (ETDEWEB)

    Shiomi, Susumu; Iwata, Yoshinori; Sasaki, Nobumitsu [Osaka City Univ. (Japan). Medical School] (and others)

    1999-08-01

    Scintigraphy with {sup 99m}Tc-diethylenetriaminepentaacetate with galactosyl human serum albumin ({sup 99m}Tc-GSA) and per-rectal portal scintigraphy are useful for evaluating hepatic functional reserve and portal circulation, respectively. We did the procedures simultaneously in some patients to examine the relationship between hepatic functional reserve and portal circulation in chronic liver disease. Scintigraphy with {sup 99m}Tc-GSA was done in 10 healthy subjects, 45 patients with chronic hepatitis, and 165 patients with cirrhosis. Fifty-seven patients (13 with hepatitis and 44 with cirrhosis) also underwent per-rectal portal scintigraphy with {sup 99m}Tc-pertechnetate within two weeks. A receptor index was calculated by dividing the radioactivity of the liver region of interest (ROI) by that of the liver-plus-heart ROI at 15 min after the injection of {sup 99m}Tc-GSA. The index of blood clearance was calculated by dividing the radioactivity of the heart ROI at 15 min by that of the heart ROI at 3 min. A solution containing {sup 99m}Tc-pertechnetate was instilled into the rectum, and serial scintigrams were taken while radioactivity curves for the liver and heart were recorded sequentially. A per-rectal portal shunt index was determined by calculating the ratio of counts for the liver to counts for the heart integrated for 24 seconds immediately after the appearance of the liver time-activity curve. The median receptor index was lower for more severe liver disorders, increasing in the order of chronic hepatitis, compensated cirrhosis and decompensated cirrhosis, and the median index of blood clearance was higher. The median receptor index was significantly lower when a complication (varices, ascites, or encephalopathy) was present, and the median index of blood clearance was higher. The shunt index was correlated significantly with the two other indices, but these values for some one-third of the patients disagreed in either indices. Scintigraphy with {sup 99m

  15. Technetium-99m ceftizoxime kit preparation

    Directory of Open Access Journals (Sweden)

    Simone Odília Fernandes Diniz

    2005-10-01

    Full Text Available The aim of this work was to prepare a kit of 99mTc-ceftizoxime (99mTc-CFT, with stability and biological activity preserved, able to identify a septic focus (E. coli in the experimental infection model in rats. The preparation of the CFT kit involved the use of lyophilized solutions containing the antibiotic ceftizoxime and the sodium dithionite reducing agent (6.0 mg/mL. After lyophilization, the kit was reconstituted with 1.0 mL of sodium 99mTc-pertechnetate solution (Na99mTcO4- with an activity of 370 MBq. The solution was boiled for 10 min and filtered through a cellulose ester filter. The labeling efficiency was on the order of 92%, remaining stable for six hours and the kit remained stable for two months. The biological activity of the 99mTc-CFT was evaluated by diffusion in agar impregnated with E.coli and S. aureus. Seven Wistar rats, weighing from 200 to 250 g, were used for the development of the septic focus. After 24 hours from the induction of the infectious site (E.coli, the animals were anesthetized and 0.1 mL of 99mTc-CFT (37 MBq was injected into the tail veins of the animals. The images were obtained with a gamma camera one, two and six hours after injection and the regions of interest (ROIs were calculated. The diameters of the inhibition halos for 99mTc-CFT were 27.16 ± 0.23 and 27.17 ± 0.20 for S.aureus and E.coli, respectively, while those for the unlabeled CFT were 30.4 ± 0.33 and 29.43 ± 0.26, respectively. The results for the biodistribution of 99mTc-CFT in infected animals furnished a ratio of 1.97 ± 0.31, 2.10 ± 0.42 and 2.01 ± 0.42 for cpm-target/cpm-no target for the one, two and six-hour periods, respectively. The images showed a clear uptake of labeled antibiotic (99mTc-CFT by the infectious site during the experiment. The results attest to the viability of producing a kit with 99m technetium-labeled ceftizoxime for the investigation of infectious processes.O objetivo deste trabalho foi preparar um kit de Tc

  16. Under used technetium-99m generators

    International Nuclear Information System (INIS)

    Health care reform truly has become a global issue and it will undoubtedly have a dramatic impact on the future of nuclear medicine business in particular. A bigger concern within the nuclear medicine community is its competitiveness with other modalities and cost effectiveness.Technetium-99m and its generators are playing key role for the majority of diagnostic scans performed in the world today. Availability of ''9''9''mTc can be increased if it is separated from ''9''9Mo after much shorter growth times. After proper planning with the extra ''9''9''mTc, a significant number of scans can be performed or we would be able to order approximately 30% low activity ''9''9Tc generators to fulfill our requirements

  17. {sup 99m}Tc-glucarate kinetics differentiate normal, stunned, hibernating, and nonviable myocardium in a perfused rat heart model

    Energy Technology Data Exchange (ETDEWEB)

    Okada, David R. [University of Pennsylvania School of Medicine, Philadelphia, PA (United States); Liu, Zhonglin [University of Arizona School of Medicine, Tucson, AZ (United States); Johnson, Gerald; Okada, Robert D. [University of Oklahoma Health Sciences Center, Oklahoma, OK (United States); University of Tulsa, Tulsa, OK (United States); Beju, Delia [Oklahoma State University School of Medicine, Tulsa, OK (United States); Khaw, Ban An [Northeastern University, Boston, MA (United States)

    2010-10-15

    {sup 99m}Tc-glucarate is an infarct-avid imaging agent. However, patients may have mixtures of normal, irreversibly injured, stunned, and hibernating myocardium. The purposes were to determine {sup 99m}Tc-glucarate uptake and clearance kinetics in these four conditions, and its ability to determine the extent of injury. Twenty-two perfused rat hearts were studied: controls (n = 5), stunned (n = 5; 20-min no-flow followed by 5-min reflow), hibernating (n = 6; 120-min low flow at 4 ml/min), and ischemic-reperfused (n = 6; 120-min no-flow followed by reflow). {sup 99m}Tc-glucarate was then infused. Tracer activity was monitored using a NaI scintillation detector and a multichannel analyzer. Creatine kinase, electron microscopy, and triphenyltetrazolium chloride determined viability. {sup 99m}Tc-glucarate 10-min myocardial uptake was significantly greater in ischemic-reperfused (2.50 {+-} 0.09) (cpm, SEM) than in control (1.74 {+-} 0.07), stunned (1.68 {+-} 0.11), and hibernating (1.59 {+-} 0.11) (p < 0.05). Tracer retention curves for ischemic-reperfused were elevated at all time points as compared with the other groups. {sup 99m}Tc-glucarate 60-min myocardial uptake was significantly greater in ischemic-reperfused (7.60 {+-} 0.63) than in control (1.98 {+-} 0.15), stunned (1.79 {+-} 0.08), and hibernating (2.33 {+-} 0.15) (p < 0.05). The 60-min well-counted tracer activity ratio of ischemic-reperfused to control was 9:1 and corroborated the NaI detector results. Creatine kinase, triphenyltetrazolium chloride, and electron microscopy all demonstrated significantly greater injury in ischemic-reperfused compared to the other groups. An excellent correlation was observed between viability markers and tracer activity (r = 0.99 triphenyltetrazolium chloride; r = 0.90 creatine kinase). {sup 99m}Tc-glucarate activity continually and progressively increased in irreversibly injured myocardium. {sup 99m}Tc-glucarate uptake was strongly correlated with myocardial necrosis as

  18. Biodistribution and dosimetry of 99mTc-ciprofloxacin, a promising agent for the diagnosis of bacterial infection

    International Nuclear Information System (INIS)

    This study reports on the biodistribution and dosimetry of technetium-99m ciprofloxacin, a radio-ligand developed for the visualisation of bacterial infection. Whole body scans were performed up to 24 h after intravenous injection of 370 MBq 99mTc-ciprofloxacin in three male and three female volunteers. Blood samples were taken at various times up to 24 h after injection. Urine was also collected up to 24 h after injection, allowing calculation of renal clearance and interpretation of whole body clearance. Time-activity curves were generated for the thyroid, heart, liver and whole body by fitting the organ-specific geometric mean counts, obtained from regions of interest. The MIRD formulation was applied to calculate the absorbed radiation doses for various organs. The images showed rapid, predominantly urinary excretion of 99mTc ciprofloxacin, with low to absent brain, lung and bone marrow uptake and low liver uptake and excretion. Accordingly, imaging conditions are excellent for both the thoracic and the abdominal region, even at early time points (60 min) post injection. In none of the volunteers was the gallbladder visualised. Approximately 60% of the injected activity was recovered in urine by 24 h post injection. The highest absorbed doses were received by the urinary bladder wall, the thyroid, the upper large intestine, the lower large intestine and the uterus. The estimated mean effective dose for the adult subject, taking into account the weight factors of the ICRP60 publication, was 0.0083 mSv/MBq. The amount of 99mTc ciprofloxacin required for adequate planar and tomographic imaging results in an acceptable effective dose to the patient. (orig.)

  19. Imaging prostatitis with Tc-99m ciprofloxacin

    International Nuclear Information System (INIS)

    Aim: Tc-99m ciprofloxacin (Infecton) imaging was developed to discriminate infection from inflammation. It claims to show an area of viable, proliferating bacterial population that is actively using DNA gyrase, which the ciprofloxacin inhibits. The application of this imaging has been reported in patients with infected arthroplasty, soft tissue infection, pelvic inflammatory diseases, etc. Clinical evaluation with conventional urological laboratory tests often provides insufficient data in differentiating chronic bacterial prostatitis (CBP) from pelvic pain syndrome, which causes inappropriate or inadequate antibiotic therapy without definite evidence of presence or absence of bacterial infection. Therefore, we investigated the diagnostic value of the Tc-99m ciprofloxacin imaging for CBP by comparing the results of the imaging with those from the conventional tests. Materials and Methods: We custom-formulated a Tc-99m ciprofloxacin kit. We used stannous tartrate for a reducing agent in making the kit. SPECT images of the region of interest were obtained at 3 hours after injection of the radiopharmaceutical. For positive control, we imaged 2 patients: acute prostatitis and urethritis. Then the actual study included 20 subjects. We excluded from the study the patients who had received antibiotic therapy within 6 weeks. The conventional tests included CBC, urinalysis, 4-glass tests, microscopic examination of expressed prostatic secretion, bacterial culture, or PCR for chlamydia trachomatis. Kappa statistics were obtained for comparison of the two tests. Results: By conventional tests, there were 10 subjects with CBP and 10 normal subjects. The typical pattern in the Tc-99m ciprofloxacin imaging indicating CBP was the increased uptake around the prostatic urethra. Five of the 10 subjects with CBP diagnosed by conventional tests showed the increased uptake to be positive cases in the Tc-99m ciprofloxacin imaging. Of the 10 subjects diagnosed as normal by the

  20. Gentc99m, computational system for the technetium-99m generator

    International Nuclear Information System (INIS)

    The technetium-99m generator is one of the main products of the PPR, as the continuity of the technetium-99m generator production is important for supporting the development of nuclear medicine. GENTC99M has been made for computational for the technetium-99m generator and includes data processing, documentation and information GENTC99M is also very useful in quality control application especially for the determinations of yield and radionuclidic impurities which consume much time. microsoft visual basic for MS-DOS and visual basic for windows have been used for making GENTC99M. Microsoft visual basic has several features that make it an ideal development language for both MS-DOS and Microsoft Windows. These features not only increase productivity, they also provide all the tools and hooks needed to develop some very sophisticated applications. for a production centre like PPR, GENTC99M is very useful to support the data processing, documentation and information system of the technetium-99m generator and it can also be modified for other products

  1. Comparative 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin uptake in human soft tissue sarcoma cell lines

    International Nuclear Information System (INIS)

    The uptake characteristics of technetium-99m hexakis-2-methoxyisobutylisonitrile (MIBI), 99mTc-tetrofosmin and 99mTc-furifosmin in human soft tissue sarcoma cell lines were investigated and compared. After 10-120 min of incubation at 37 C, 32 C and 22 C with 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin, the kinetics of cellular uptake of these tracers in human soft tissue sarcoma cells SW 684 (fibrosarcoma), SW 872 (liposarcoma), SW 982 (synovial sarcoma) and SW 1353 (chondrosarcoma) was assessed. The uptake of 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin was temperature dependent. The kinetics of uptake of 99mTc-MIBI and of 99mTc-tetrofosmin was similar between fibrosarcoma and liposarcoma cells, as well as between synovial sarcoma and chondrosarcoma cells. 99mTc-furifosmin showed similar uptake kinetics in all cell lines. The uptake of 99mTc-furifosmin was, however, significantly higher in liposarcoma than in the other cells. The data indicate that the cellular uptake of 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin is dependent on cellular metabolic activity. (orig.)

  2. Combined Tc-99m sesta MIBI scin

    OpenAIRE

    Enas Abdel Ghany Ibrahim; Momena Essam Elsadawy

    2015-01-01

    Purpose: To evaluate the role of combined high resolution ultrasonography and technetium Tc-99m methoxyisobutylisonitrile (MIBI) parathyroid scintigraphy in preoperative detection and localization of parathyroid adenoma, for better operative outcome. Patients and methods: 40 patients were included in this study, 25 of them were females and 15 were males, with age ranging between 31 and 75 (mean 46 ± 15). All patients presented with some of symptoms of hyperparathyroidism with venous blood ...

  3. Studies of the myocardial uptake and excretion mechanisms of a novel 99mTc heart perfusion agent

    International Nuclear Information System (INIS)

    Introduction: 99mTc-TMEOP is a novel heart perfusion radiotracer exhibiting high initial and persistent heart uptake associated with rapid blood and liver clearance. This study aimed at determining the mechanisms of myocardial localization and fast liver clearance of 99mTc-TMEOP. Methods: Subcellular distribution of 99mTc-TMEOP was determined in excised rat heart tissue by differential centrifugation. The effect of cyclosporin A on the pharmacokinetic behaviour of 99mTc-TMEOP was evaluated by both ex vivo biodistribution and in vivo planar imaging studies. Results: Subcellular distribution studies showed that more than 73% of 99mTc-TMEOP was associated with the mitochondrial fraction. Comparison with subcellular distribution of 99mTc-sestamibi showed no significant difference in the mitochondrial accumulation between the two tracers. Biodistribution studies in the presence of cyclosporin A revealed an increase in kidneys and liver uptake of 99mTc-TMEOP, suggesting the involvement of multidrug resistance transporters in determining its pharmacokinetic profile. Conclusions: The heart uptake mechanism of 99mTc-TMEOP is similar to that of the other reported monocationic 99mTc cardiac agents and is associated with its accumulation in the mitochondria. Cyclosporin A studies indicate that the fast liver and kidney clearance kinetics is mediated by P-glycoprotein (Pgp), supporting the potential interest of this radiotracer for imaging Pgp function associated with multidrug-resistant tumours.

  4. Measurement of lymphatic function with technetium-99m-labelled polyclonal immunoglobulin

    International Nuclear Information System (INIS)

    A reliable method for measuring lymph flow in physiological units would be valuable, especially in conditions in which it is uncertain whether lymph flow is increased or decreased. The requirements of a radiopharmaceutical for such measurement include stable radionuclide labelling and rapid access to lymphatic vessels following tissue injection but no access to blood vessels. A soluble macromolecule is likely to come closest to meeting these requirements. Technetium-99m- labelled human polyclonal immunoglobulin (HIG) was therefore investigated firstly in comparison with 99mTc-labelled human serum albumin (HSA) in patients undergoing routine lymphoscintigraphy and secondly with respect to injection site in a group of volunteers with post-mastectomy oedema (PMO). Subcutaneous injection of 99mTc-HIG into the web space of a distal extremity gave images in which lymphatic vessels were more clearly defined compared with images obtained after injection of 99mTc-HSA. Lymph nodes were also more clearly defined, suggesting specific retention of HIG, possibly through Fc-mediated binding. Peripheral blood sampling showed a delayed arrival in blood of radioactivity after 99mTc-HIG compared with 99mTc-HSA, although ultimately, the blood recovery of 99mTc-HIG was significantly higher (P 99mTc-HSA. Clearance rates of radioactivity from the injection site were not sinificantly different, however, between the two agents. In patients with PMO, web space injection of 99mTc-HIG gave excellent images of normal lymphatic vessels, of lymph nodes and of abnormal lymph drainage such as dermal backflow in swollen arms. In contrast, neither lymphatic vessels nor lymph nodes were visualised after injection into the skin of the dorsum of the distal forearm. Although there was no difference in clearance rates from the injection sites between normal and swollen arms with either agent in PMO, clearance was significantly faster following injection into the web space (0.11% per minute for normal and

  5. 99mTc-sestamibi and 99mTc-tetrofosmin scintigraphy in myeloma bone disease

    International Nuclear Information System (INIS)

    Full text: Evaluation of the role of scintigraphy with 99mTc-Sestamibi (99mTc-MIBI) and 99mTc-Tetrofosmin (99mTc-TF) in the detection of bone marrow involvement in patients (pts) with multiple myeloma (MM) and in follow-up. 62 pts with MM and 38 pts with monoclonal gammopathy of undetermined significance (MGUS) were enrolled in this study. Forty-seven out sixty-two MM pts had active disease (AD), 10/62 were in complete remission (CR) and 5/62 in partial remission (PR) after chemotherapy. Whole-body scans were obtained 10 min after the i.v. injection of 740 MBq of 99mTc-MIBI in anterior and posterior view. The scans were scored semiquantitatively according to extension and intensity of tracer uptake. All 38 MGUS pts had a negative 99mTc-MIBI scan. As to the MM pts, 49/62 pts (44 with AD, 4 with PR and 1 with CR) had a positive 99mTc-MIBI scan, while the 99mTc-MIBI scan was negative in 13/62 pts (9 with CR, 1 with PR and 3 with AD). The overall sensitivity of the scintigraphic procedure was 92 % while specificity was 97 %. A total of 42 follow-up scans with 99mTc-MIBI were performed in 30 MM pts after high-dose chemotherapy, with an average follow-up duration of 14.6 ± 8.9. The follow-up 99mTc-MIBI scintigraphy was positive in 14/15 of the AD pts, while 6/8 negative scans were observed in the CR pts; the seven PR pts exhibited in five cases a negative scan and in two a mild scintigrafic positivity. The overall sensitivity and specificity of the follow-up scan were, respectively, 86 % and 75 %. In six patients, one at the diagnosis and five showing a positive 99mTc-MIBI scan during the follow-up, 740 MBq of 99mTc-TF was administered within two days with the same acquisition protocol. The scans provided substantially identical information. 99mTc-TF provided a mildly higher contrast between lesion and background activities. The results obtained in this study provide additional evidence indicating that 99mTc-MIBI scintigraphy closely reflects myeloma disease activity in

  6. Glomerular filtration rate estimated from the uptake phase of 99mTc-DTPA renography in chronic renal failure

    DEFF Research Database (Denmark)

    Petersen, L J; Petersen, J R; Talleruphuus, U;

    1999-01-01

    The purpose of the study was to compare the estimation of glomerular filtration rate (GFR) from 99mTc-DTPA renography with that estimated from the renal clearance of 51Cr-EDTA, creatinine and urea.......The purpose of the study was to compare the estimation of glomerular filtration rate (GFR) from 99mTc-DTPA renography with that estimated from the renal clearance of 51Cr-EDTA, creatinine and urea....

  7. Permeability of the bronchial mucosa to /sup 99m/Tc-DTPA in asthma

    International Nuclear Information System (INIS)

    Previous investigators, using 99mTc-DTPA aerosol as a marker to assess epithelial permeability in asthma, did not find an increased permeability in this group. However, they either failed to deliver the aerosol to the optimal site (bronchial mucosa, not alveoli) or failed to account for mucociliary clearance in analyzing their results. We studied 10 asthmatics and eight age-matched control subjects using a dosimeter (Spira-Elektra 2) and a carefully controlled breathing pattern to deliver aerosol to the subjects' airways. Two aerosols were delivered on separate days in each patient; 99mTc-DTPA aerosol, and 99mTc-HSA (human serum albumin), using similar breathing patterns to ensure reproducibility of the deposition pattern with the two aerosols. From measurements of retention versus time over a 1-h period, rate constants Ktot and Km were determined for the clearance of 99mTc-DTPA and 99mTc-HSA, respectively. By modelling the airways as a single compartment with two possible routes of clearance, we determined the permeability rate constant, Kp, as Ktot minus Km. There was no significant difference between Ktot in normal subjects and asthmatics; however, because of the slower mucociliary clearance in the asthmatic group, and the relative importance of mucociliary clearance in determining the washout of 99mTc-DTPA aerosol, there was a significant difference in airway permeability between the normal subjects and the asthmatics (t1/2 = 296 min +/- 141 SD and 126 min +/- 58, p less than 0.01, in normal subjects and asthmatics, respectively)

  8. Technetium-99m pyridoxylideneglutamate (P. G. ) cholescintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Stadalnik, R.C.; Matolo, N.M.; Jansholt, A.L.; Krohn, K.A.; DeNardo, G.L.; Wolfman, E.F Jr.

    1976-12-01

    Technetium-99m P.G. cholescintigraphy was performed in 27 human volunteers and 81 patients referred for hepatobiliary tract disease. The gallbladder, biliary system, and gastrointestinal tract were well visualized in the normal patients and volunteers. The gallbladder was not visualized in 22 patients with histologically proved cholecystitis with cystic duct obstruction. Nine patients with complete extrahepatic obstruction of the common bile duct were correctly diagnosed. Hepatocellular disease and incomplete obstruction, with and without jaundice, were diagnosed with this technique. Oral cholecystography is superior to this method for the detection of cholelithiasis in nonjaundiced patients.

  9. /sup 99m/Tc-aprotinin: A new tracer for kidney morphology and function

    Energy Technology Data Exchange (ETDEWEB)

    Bianchi, C.; Donadio, C.; Tramonti, G.; Lorusso, P.; Bellitto, L.; Lunghi, F.

    1984-01-01

    Aprotinin (Ap), a low molecular weight polyeptide (6500 dalton), is a protease inhibitor which is electively and stably accumulated in the kidney. In 112 adult patients, with either uni- or bilateral renal disease with different degrees of renal impairment (from normal GFR to advanced renal failure), renal scans were performed by means of Ap labelled with /sup 99m/Tc. Highly satisfactory renal scans were obtained in all patients. In 20 patients with renal failure (serum creatinine 1.8 - 8.5 mg/dl, mean 4.7) a comparison was made of the renal scans obtained with /sup 99m/Tc-Ap and with /sup 99m/Tc-DMSA. /sup 99m/Tc-Ap was slightly better than /sup 99m/Tc-DMSA, especially in patients with far advanced renal failure. Some aspects of the pharmacokinetics of /sup 99m/Tc-Ap were studied in 72 cases. In 22 of these patients plasma clearance of /sup 99m/Tc-Ap was determined by the single injection method using a two-compartment model. In patients with GFR>90 ml/min plasma cl of /sup 99m/Tc-Ap was 67.6 +- 8.4 SD ml/min. A good correlation was observed between plasma clearance of /sup 99m/Tc-Ap and GFR (r = 0.74). After i.v. injection /sup 99m/Tc-Ap was stably fixed by the kidney. Renal radioactivity remained stable between the 2nd and the 8th hour after the injection. Urinary excretion of radioactivity measured in 35 patients in the first and in the second 2-hour interval after i.v. injection of /sup 99m/Tc-Ap was negligible in all patients (2.7 +- 1.5 SD percent of the dose in the fist 2 hours; 2.8 +- 1.4 SD between the 2nd and the 4th hour). Conclusions. /sup 99m/Tc-Ap is an excellent agent for renal imaging. It also seems promising for renal function studies.

  10. Early detection of amphotericin B induced nephrotoxicity by 99mTc-DTPA: a useful test

    International Nuclear Information System (INIS)

    Amphotericin B (AMB) with a broad spectrum of antifungal activity is used for the treatment of life-threatening mycoses, especially in immunocompromised patients. Since measurements of the blood level of AMB and of creatinine do not provide early warning of AMB induced renal toxicity, we studied the effects of AMB on biodistribution of a glomerular agent, 99mTc-DTPA. In Swiss mice, the toxicity of AMB was studied at single intravenous doses of 0.5-3.5 mg/kg body weight. Dose dependent effects consisted of decreased blood clearance and urinary excretion. Recovery of function was shown in dose ranges corresponding to high level clinical schedules. Serum creatinine changes lagged behind the99m Tc-DTPA alterations. This suggests that AMB toxicity might be monitored by blood/urinary clearance of 99mTc-DTPA

  11. Pharmacokinetics evaluation of Technetium{sup 99m} Ethylene-di-cysteine diethyl ester radiopharmaceutical; Avaliacao farmacocinetica do radiofarmaco etilenodicisteina dietil este'-{sup 99m} Tc (ECD-{sup 99m} Tc)

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Elaine Bortoleti de; Carvalho, Olga G. de; Muramoto, Emiko; Almeida, Maria A.T.M. de; Mesquita, Carlos H. de [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)

    1996-07-01

    The biological distribution studies of {sup 99m} Tc-ECD in mice were performed in this work. Using ANACOMP computer program, a composed by seven compatiments was adjusted, characterized by a fast blood clearance, an undirectional renal depuration and intestinal elimination, determined by intense hepato biliary transit. Plasma, bile and urinary samples were analysed by HPLC, showing the in vivo metabolism of the compound. (author)

  12. Possible role of 99mTc-Dextran (99mTc-Dx) in detection of occult small intestinal lesions

    International Nuclear Information System (INIS)

    Two cases are presented wherein 99mTc-dextran (99mTc-Dx) imaging localised focal pathology in the small intestine and contributed in reaching a preoperative diagnosis. In the first case, 99mTc-pertechnetate and 99mTc-Dx accumulated in a suspected Meckel's diverticulum equally well. 99mTc-Dx additionally identified exudative discharge from the lesion. In the second case, 99mTc-Dx identified a distal ileal lesion later proved to be a non-specific ulcer on surgery. 99mTc-Dx scanning may have a role in imaging of occult intestinal lesions. (author)

  13. Bioavailability of {sup 99m}Tc-paclitaxel-glucuronide ({sup 99m}Tc-PAC-G)

    Energy Technology Data Exchange (ETDEWEB)

    Biber Muftuler, F.Z.; Demir, I.; Uenack, P.; Ichedef, C.; Yurt Kilcar, A. [Ege Univ., Izmir (Turkey). Dept. of Nuclear Applications

    2011-07-01

    An antitumor agent paclitaxel (PAC) has been proved to be efficient in the treatment of breast and ovarian cancer. Glucuronic acid-derived paclitaxel compound (paclitaxel-glucuronide (PAC-G)) was enzymatically synthesized using microsome preparate separated from rat livers. The biodistribution mechanism of PAC-G in healthy female Albino Wistar rats has been investigated. The expected structure is confirmed according to LC/MS results, and the possible attachment is to C2-hydroxyl group. PAC-G was labeled with {sup 99m}Tc and the radiochemical yield of radiolabeled compound ({sup 99m}Tc-PAC-G) was 98.0 {+-} 02.74% (n=9). The range of the breast/blood and breast/muscle ratios is approximately between 3 and 35 in 240 min. All these experimental studies indicate that {sup 99m}Tc-PAC-G may potentially be used in breast tissue as an imaging agent. (orig.)

  14. Tc- 99 m gel generator; an alternative source of Tc-99 m for medical uses

    International Nuclear Information System (INIS)

    Tc-99 m is widespread radioisotope in nuclear medicine and it can be produced by two different methods. The first method is performed by chromatographic genera tory by using Mo-99 prepared by fission of U-235, and another method is activation of Mo-99 can be complexed by ZrOCl2 and produce a gel. Mo-99 remains in this gel and Tc-99 m elute with normal saline solution. Many factors such as P H, concentration of Mo in the gel structure, and drying conditions of gel affect on the efficiency of the generator. In this research the gel generator production and some factors which are effecting on the 99 m Tc production are presented

  15. Preparation and preclinical pharmacological study on a novel bone imaging agent {sup 99m}Tc-EMIDP

    Energy Technology Data Exchange (ETDEWEB)

    Lin Jianguo [Key Laboratory of Nuclear Medicine, Ministry of Health and Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063 (China); Luo Shineng, E-mail: shineng914@yahoo.com.c [Key Laboratory of Nuclear Medicine, Ministry of Health and Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063 (China); Chen Chuanqing [Key Laboratory of Nuclear Medicine, Ministry of Health and Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063 (China); School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122 (China); Qiu Ling, E-mail: qiulingwx@gmail.co [Key Laboratory of Nuclear Medicine, Ministry of Health and Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063 (China); Wang Yan [Key Laboratory of Nuclear Medicine, Ministry of Health and Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063 (China); School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122 (China); Cheng Wen; Ye Wanzhong [Key Laboratory of Nuclear Medicine, Ministry of Health and Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063 (China); Xia Yongmei [School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122 (China)

    2010-09-15

    A novel zoledronic acid (ZL) derivative, 1-hydroxy-2-(2-ethyl-4-methyl-1H-imidazol-1-yl)ethane-1,1-diyldiphosphonic acid (EMIDP), was prepared and labeled with {sup 99m}Tc successfully in a high labeling yield and good stability in vitro. The preclinical pharmacological properties of {sup 99m}Tc-EMIDP were investigated and compared with {sup 99m}Tc-MDP and {sup 99m}Tc-ZL. The studies of biodistribution in mice and SPECT bone imaging of the rabbit suggest that {sup 99m}Tc-EMIDP has highly selective uptake in the skeletal system and rapid clearance in the soft tissues. The present findings indicate that {sup 99m}Tc-EMIDP holds great potential for bone scintigraphy.

  16. In vitro incorporation studies of 99mTc-alendronate sodium at different bone cell lines

    International Nuclear Information System (INIS)

    Bisphosphonates can be labeled with Technetium-99m (99mTc) and are used for bone imaging because of their good localization in the skeleton and rapid clearance from soft tissues. Over the last decades bone scintigraphy has been used extensively in the evaluation of oncological patients to provide information about the sites of bone lesions, their prognosis and the effectiveness of therapy by showing the sequential changes in tracer uptake. Since the lesion visualization and lesion/bone ratio are important utilities for a bone scanning radiopharmaceutic; in this study incorporation of 99mTc labeled alendronate sodium (99mTc-ALD) was evaluated in U2OS (human bone osteosarcoma) and NCI-H209 (human bone carcinoma) cell lines. ALD was directly labeled by 99mTc, radiochemical purity and stability of the complex were analyzed by radioactive thin layer chromatography and radioactive high performance liquid chromatography studies. For cell incorporation study, NCI-H209 and U2OS cell lines were used with standard cell culture methods. The six well plates were used for all experiments and the integrity of each cell monolayer was checked by measuring its transepithelial electrical resistance (TEER) with an epithelial voltammeter. Results confirmed that ALD was successfully radiolabeled with 99mTc. 99mTc-ALD incorporated with NCI-H209 and U2OS cells. The uptake percentages of 99mTc-ALD in NCI-H209 and U2OS cell lines were found significantly different. Since 99mTc-ALD highly uptake in cancer cell line, the results demonstrated that radiolabeled ALD may be a promising agent for bone cancer diagnosis. (author)

  17. /sup 99m/Tc(Sn)-N-pyridoxylaminates: a new series of hepatobiliary imaging agents

    International Nuclear Information System (INIS)

    Three derivatives of N-pyridoxylamino acid were synthesized: /sup 99m/Tc(Sn)-N-pyridoxylphenylalanine, -N-pyridoxyltryptophan, and -N-pyridoxyl-5-methyltryptophan. The chemical and biologic properties of each complex was found to be quite analogous to those of the corresponding /sup 99m/Tc(Sn)pyridoxylideneaminate. In rats, /sup 99m/Tc(Sn)-N-pyridoxyl-5-methyltryptophan [/sup 99m/Tc(Sn)PHMT] showed rapid blood clearance, fast hepatobiliary transit, low urinary excretion, and no intestinal reabsorption. Over 90% of the dose arrived in the intestine through the liver at 30 min after i.v. administration, whereas only 2% of the dose escaped through the kidneys. In rabbits the gallbladder was clearly visualized within 5 min of injection and no renal or bladder images were noted on any scintiphotos. The BSP (sulfobromophthalein) intervention study revealed that the biliary excretion of /sup 99m/Tc(Sn)PHMT is much more resistant than that of /sup 99m/Tc(Sn) diethyl-IDA to the intervention of BSP and hence to serum bilirubin. Toxicity studies on PHMT and Sn-PHMT indicated a wide margin of safety for the proposed human dose

  18. Usefulness of renal dynamic function study with [sup 99m]Tc-MAG[sub 3] (mercaptoacetylglycylglycylglycine)

    Energy Technology Data Exchange (ETDEWEB)

    Shirakawa, Seishi; Tamaki, Nagara; Torizuka, Tatsuo; Fujita, Toru; Yano, Shinsuke; Tsuchimochi, Shinsaku; Yonekura, Yoshiharu; Konishi, Junji; Terai, Akito (Kyoto Univ. (Japan). Faculty of Medicine)

    1994-08-01

    The clinical value of a newly developed renography agent [sup 99m]Tc-mercaptoacetylglycylglycylglycine ([sup 99m]Tc-MAG[sub 3]) was assessed in comparison with [sup 123]I-orthoiodohippurate ([sup 123]I-OIH). Clear perfusion images were obtained early after [sup 99m]Tc-MAG[sub 3] administration due to physical advantages of the tracer. A close correlation was observed of T[sub max] and T[sub 1/2] values between [sup 99m]Tc-MAG[sub 3] and [sup 123]I-OIH. However, T[sub 1/2] calculated by [sup 99m]Tc-MAG[sub 3] was significantly longer than that by [sup 123]I-OIH. In addition, the effective renal plasma flow (ERPF) value calculated by the clearance rate of the tracer by Tauxe method (radioactivity in plasma at 43 minutes after tracer administration) was smaller than that by [sup 123]I-OIH. In conclusion, [sup 99m]Tc-MAG[sub 3] is considered as a useful agent for renography. (author).

  19. Effects of radiofrequency ablation on individual renal function: assessment by technetium-99m mercaptoacetyltriglycine renal scintigraphy.

    Directory of Open Access Journals (Sweden)

    Nasu,Yasutomo

    2006-04-01

    Full Text Available We quantitatively evaluated total and individual renal function by technetium-99m mercaptoacetyltriglycine (Tc-99m MAG3 renal scintigraphy before and after radiofrequency ablation (RFA of renal tumors. Eleven patients who underwent Tc-99m MAG3 renal scintigraphy 1 week before and after RFA were evaluated (7 men and 4 women ; age range : 23-83 years ; mean age : 60.6 years. Five patients had solitary kidneys, and five had normally or minimally functioning contralateral kidneys. One patient had a renal cell carcinoma in the contralateral kidney. One patient with a solitary kidney underwent RFA a second time for a residual tumor. In patients with a solitary kidney, MAG3 clearance decreased after 5 of 6 RFAs, and in patients with a normally functioning contralateral kidney, MAG3 clearance decreased after 4 of 5 RFAs, but no significant differences were observed between before and after treatments. In addition to the total MAG3 clearance, the split MAG3 clearance was evaluated in patients with a normally functioning contralateral kidney. MAG3 clearance decreased in 4 of 5 treated kidneys, while it adversely increased in the contralateral kidneys after 4 of 5 RFAs. No significant differences, however, were observed between before and after treatments. The results of our study revealed no significant differences in sCr, BUN, CCr, or MAG3 clearance between pre- and post-RFA values. These results support data regarding the functional impact and safety of renal RFA in published reports. We evaluated total and individual renal function quantitatively using Tc-99m MAG3 renal scintigraphy before and after treatment. This scintigraphy was very useful in assessing the effects of RFA on renal function.

  20. Phase II clinical study on sup 99m Tc-GSA, a new agent for functional imaging of the liver

    Energy Technology Data Exchange (ETDEWEB)

    Torizuka, Kanji (Fukui Medical School (Japan)); Ha-Kawa, S.K.; Kudo, Masatoshi; Kitagawa, Shinichi; Kubota, Yoshitsugu; Tanaka, Yoshimasa; Hino, Megumu; Ikekubo, Katsuji

    1992-01-01

    Phase II study of {sup 99m}Tc-DTPA-galactosyl human serum albumin ({sup 99m}Tc-GSA), a new radiopharmaceutical which binds to the asialoglycoprotein receptors on the hepatocytes, was performed in 81 patients with liver diseases to validate its safety and possibility for the evaluation of hepatic function. None of adverse reactions, abnormal clinical laboratory findings and anti-{sup 99m}Tc-GSA antibody production due to {sup 99m}Tc-GSA was recognized. Immediately after the injection of {sup 99m}Tc-GSA, the dynamic data and serial hepatic images were obtained for 60 min. The indices for blood clearance and liver accumulation were calculated based on the counts in the regions of interest on the hearts and livers. In 54 patients with chronic hepatic disorders such as liver cirrhosis, the blood clearance and liver accumulation of {sup 99m}Tc-GSA were retarded according to the progress of the hepatic disorders. The findings of {sup 99m}Tc-GSA scintigraphy also reflected the hepatic functions of the patients with large hepatic tumors, obstructive jaundice and acute hepatitis. These results suggest that {sup 99m}Tc-GSA has the clinical potentials to evaluate the liver functions in the patients with hepatic disorders. (author).

  1. Process modifications of obtaining Tc-99m by solvent extraction

    International Nuclear Information System (INIS)

    This paper describes a modification in the process to obtaining Tc-99m by the extraction method of solvent from Mo-99 produced by irradiation. Tc-99m is considered an ideal radionuclide for medical and biological applications

  2. Current status of Tc-99m production in Thailand

    Energy Technology Data Exchange (ETDEWEB)

    Charoen, Sakda [Office of Atomic Energy for Peace, Bangkok (Thailand)

    2003-03-01

    Technetium-99m is the workhorse of nuclear medicine and currently accounts for over 80% of all in vivo diagnostic procedures. In Thailand, Office of Atomic Energy for Peace (OAEP) had experiences in production of Technetium-99m by solvent extraction process and alternative technology for Technetium-99m generator based on zirconium molybdate and titanium molybdate gel generators were also studied. The paper describes past experiences and future plan of Technetium-99m production in Thailand. (author)

  3. Technetium-99m-dimethylglyoxime ([sup 99m]Tc-DMG) as renal imaging agent

    Energy Technology Data Exchange (ETDEWEB)

    Adonaylo, V.N. (Buenos Aires Univ. (Argentina). Facultad de Ciencias Exactas y Naturales Buenos Aires Univ. (Argentina). Dept. de Ciencias Biologicas); Stahl, Adriana; Pomilio, A.B.; Vitale, A.A. (Buenos Aires Univ. (Argentina). Facultad de Ciencias Exactas y Naturales); Canellas, C.O. (Buenos Aires Univ. (Argentina). Facultad de Ciencias Exactas y Naturales Comision Nacional de Energia Atomica, Buenos Aires (Argentina))

    1993-06-01

    Dimethylglyoxime (DMG) labelled with [sup 99m]Tc is presented as a renal imaging agent. The behaviour of this complex was analysed at different pH by means of UV spectral data and using DMG-calcium chloride as a reference complex. Biokinetic data were evaluated in two biological models, Sprague-Dawley rats and Didelphis albiventris argentine opossum. Biodistribution in rats demonstrated fast and specific renal excretion. Time-activity values over both kidneys could be quantified for this complex. Renographic studies led to mean time-to maximum values on twelve assays of 2.0 [+-] 0.1 min and a mean relative function of 53.0 [+-] 2.3 and 47.0 [+-] 3.2 for right and left kidneys, respectively. [sup 99m]Tc-DMG showed specificity for the renal excretion pathway and therefore seems to be a very useful radiopharmaceutical for renal function studies. (Author).

  4. Technetium-99m-dimethylglyoxime (99mTc-DMG) as renal imaging agent

    International Nuclear Information System (INIS)

    Dimethylglyoxime (DMG) labelled with 99mTc is presented as a renal imaging agent. The behaviour of this complex was analysed at different pH by means of UV spectral data and using DMG-calcium chloride as a reference complex. Biokinetic data were evaluated in two biological models, Sprague-Dawley rats and Didelphis albiventris argentine opossum. Biodistribution in rats demonstrated fast and specific renal excretion. Time-activity values over both kidneys could be quantified for this complex. Renographic studies led to mean time-to maximum values on twelve assays of 2.0 ± 0.1 min and a mean relative function of 53.0 ± 2.3 and 47.0 ± 3.2 for right and left kidneys, respectively. 99mTc-DMG showed specificity for the renal excretion pathway and therefore seems to be a very useful radiopharmaceutical for renal function studies. (Author)

  5. The single-plasma-sample method for determining the glomerular filtration rate with Tc-99m-diethylenetriamine pentaacetic acid in childhood and adolescence. Is it age-dependent?

    International Nuclear Information System (INIS)

    The aim of this study is to assess the accuracy of the pre-existing single-plasma-sample method (SPSM) to measure the glomerular filtration rate (GFR) with Tc-99m-diethylenetriamine pentaacetic acid (Tc-99m-DTPA) in children and adolescents. In addition, the age-independent SPSM is evaluated with two algorithms (Bubeck and Russell) applied for Tc-99m-mercaptoacetyltriglycine (Tc-99m-MAG3) SPSM. The study was performed on 14 patients (12 men and 2 women; age range 3 to 19 yr) with renal diseases. Tc-99m-DTPA (5 MBq/kg) was injected intravenously and thereafter blood samples were taken at 5, 15, 60, 90, 120, 150 and 180 min via the indwelling tube. Radioactivity in the injection syringe and plasma was measured by means of a double-well single-plastic scintillation counter. The true'' GFR as a reference was determined by two methods: 2-exponential curve fitting 7 samples (GFR7) and 1-exponential curve fitting 3 samples between 90 and 150 min (GFR3) in a slow clearance phase. The GFR7 and GFR3 were searched for to the clearance (GFR1) estimated from a plasma concentration at various sample times by means of 3 equations designed for children (Groth and Aasted, Ham-I and -II) and 3 for adults (Christensen and Groth, Jacobsson, Itoh). All the SPSM showed close correlations (r>0.95) with the reference methods. Among them, Jacobsson's equation at sample time=120 min tended to be the most accurate (r=0.9826, root mean squared error (RMSE)=7.8 ml/min)). On the other hand, Ham-I's equation at sample time=120 min was the most accurate, when it was referred to GFR3 in correction for overestimation (r=0.9951, RMSE=4.60 ml/min). The Bubeck and Russells' algorithms showed that the regression equation between the GFR7 and the estimates was different in 2 groups of adults (49 cases) and children/adolescents. Our study indicates that Jacobsson's and Christensen and Groth's equations designed for adults are also applicable in determining the GFR with Tc-99m-DTPA in children and

  6. Biokinetics and dosimetry in patients of {sup 99m}Tc-HYNIC-Lys{sup 3}-Bombesin: images of GRP receptors; Biocinetica y dosimetria en humanos de {sup 99m}Tc-HYNIC-Lys{sup 3}-Bombesina: imagenes de receptores GRP

    Energy Technology Data Exchange (ETDEWEB)

    Santos C, C. L. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)

    2007-07-01

    The bombesin (BN) receptor subtype 2 (GRP-r) is expressed in several normal human tissues and is over-expressed in various human tumors including breast, prostate, small cell lung and pancreatic cancer. Recently [{sup 99m}Tc]EDDA/HYNIC-Lys{sup 3}-bombesin ({sup 99m}Tc-HYNIC-BN) was reported as a radiopharmaceutical with high stability in human serum, specific cell GRP-r binding and rapid cell internalization. The aim of this study was to evaluate the feasibility of using {sup 99m}Tc-HYNIC-BN to image GRP-r and to assess the radiopharmaceutical biokinetics and dosimetry in 4 breast cancer patients and in 7 healthy women. Methods: Whole-body images were acquired at 20, 90, 180 min and 24 h after {sup 99m}Tc-HYNIC-BN administration. Regions of interest (ROIs) were drawn around source organs on each time frame. The same set of ROIs was used for all 11 scans and the cpm of each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate {sup 99m}Tc-HYNIC-BN time-activity curves in each organ in order to calculate the total number of disintegrations (N) that occurred in the source regions, according with MIRD methodology. N data were the input for the OLINDA/EXM code to calculate internal radiation dose estimates. Results: Images showed a rapid radiopharmaceutical blood clearance with renal excretion as predominant route. {sup 99m}Tc-HYNIC-BN exhibited high in vivo affinity for GRP-r over-expression successfully visualized in cancer mammary glands and well differentiated from the ubiquitous GRP-r expression in normal breast, lungs and airways. There was no statistically significant difference (p > 0.05) in the radiation absorbed doses between cancer patients and healthy women. The average equivalent doses (n=11) for a study using 740 MBq were 24.8 +- 8.8 mSv (kidneys), 7.3 +- 1.8 mSv (lungs), 6.5 +- 4.0 mSv (breast) 2.0 +- 0.3 mSv (pancreas), 1.6 +- 0.3 mSv (liver), 1.2 +- 0.2 mSv (ovaries) and 1.0 +- 0.2 mSv (red

  7. Kinetic investigations of sup(99m)Tc-labelled radiopharmaceuticals

    International Nuclear Information System (INIS)

    Several radiopharmaceuticals (sup(99m)Tc-Fe-ascorbates, sup(99m)Tc-Sn-DTPA, sup(99m)Tc-Sn-ACD-citrate-complex and sup(99m)Tc-Sn-tetracyclin-HAsc-ACD-complex) for renal and tumour scintigraphy were tested in animal experiments. Also tested was sup(99m)Tc-penicillamine for scintigraphic investigations of the gallbladder and the liver. The findings suggest that the different radiopharmaceuticals have different degrees of reliability and exactness, and that some of them should be combined to achieve better diagnostic values. (GSE/AK)

  8. 99mTc radiolabeling and biodistribution study of scorpionfish (Scorpaena plumieri) venom in Swiss mice

    International Nuclear Information System (INIS)

    The use of radiotracers in research of animal venom has been scarce, although it allows an excellent approach to follow the process of biodistribution and kinetics of toxins, and tissue distribution studies are very important for clinical use. Our group has demonstrated that Scorpaena plumieri venom (SP) possess high antitumoral activity and can be a source of template molecules for the development of antitumoral drugs. The purpose of this study was to radiolabel SP with 99mTc and investigate its biodistribution profile. High labeling yield was obtained and the results suggest that [99mTc]SP can be an useful tool for in vivo studies. The analysis indicated that SP is excreted manly by the kidneys with a slow clearance rate. The significant [99mTc]SP uptake in the heart and lungs may explain, at least partially, the pulmonary edema and effect cardiac observed by the envenoming. (author)

  9. Technetium 99 m spiperone dithiocarbamate study as a potential agent for brain pathologies diagnosis related to D 2 dopamine receptors

    International Nuclear Information System (INIS)

    Psycho-pharmacology has been discovering much about the D 2 dopamine receptors and their interrelationship to brain pathologies such as Parkinson's Disease, Schizophrenia and Huntington Disease. Those biological receptors have got affinity with dopamine endogenous agent, so that they complex and, in non pathological individuals, the biological receptors contribute to bring the levels of dopamine and free acetylcholine into equilibrium. The D 2 antagonistic psychotropic agents because of having got strong affinity with those receptors, have been being transformed into radiopharmaceuticals to diagnose these pathological disease of Central Nervous System. The Spiperone Dithiocarbamate complex studied by us, is a potential diagnosis agent because of being highly lipo-soluble and having close relationship with D 2 receptors. Besides, it is a photon emitter, allowing the use of SPECT (Single Photon Emission Computed Tomography) technique which is economically less expensive if compared to the PET (Positron Emission Tomography) technique. The Spiperone Dithiocarbamate (SPDC) is synthesized from Spiperone and its complexation with Technetium-99 m has been prepared with its reaction parameters after being studied and improved. The SPDC-99m Tc complex biological distribution have made in Wistar rats and the uptake of spleen, heart, liver stomach, lung, kidney, blood, intestine and brain have been resolved. The plasmatic clearance curve has been based on Wistar rats data and the know-how of the kit (for label SPDC with Tc) has been achieved. (author). 5 figs, 4 tabs

  10. Biokinetics and dosimetry in patients of {sup 99m}Tc-EDDA/HYNIC-Tyr{sup 3}-octreotide prepared from lyophilized kits

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez-Vazquez, Armando [Departamento de Medicina Nuclear, Hospital Militar (Mexico); Facultad de Medicina, Universidad Autonoma del Estado de Mexico (Mexico); Ferro-Flores, Guillermina [Departamento de Materiales Radiactivos, Gerencia de Aplicaciones Nucleares en la Salud, Instituto Nacional de Investigaciones Nucleares, Km. 36.5 Carretera Mexico-Toluca, Ocoyoacac, Estado de Mexico, C.P. 52045 (Mexico)]. E-mail: gff@nuclear.inin.mx; Arteaga de Murphy, Consuelo [Departamento de Medicina Nuclear, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran (Mexico); Gutierrez-Garcia, Zohar [Departamento de Medicina Nuclear, Hospital Militar (Mexico)

    2006-07-15

    {sup 99m}Tc-EDDA/HYNIC-Tyr{sup 3}-octreotide ({sup 99m}Tc-HYNIC-TOC) has shown high in vitro and in vivo stability, rapid background clearance and rapid detection of somatostatin receptor-positive tumors. The aim of this study was to establish a biokinetic model for {sup 99m}Tc-HYNIC-TOC prepared from lyophilized kits, and to evaluate its dosimetry as a tumor imaging agent in patients with histologically confirmed neuroendocrine tumors. Whole-body images from eight patients were acquired at 5, 60, 90, 180 min and 24 h after {sup 99m}Tc-HYNIC-TOC administration obtained from instant freeze-dried kit formulations with radiochemical purities >95%. Regions of interest (ROIs) were drawn around source organs on each time frame. The same set of ROIs was used for all eight scans and the count per minute (cpm) of each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate {sup 99m}Tc-HYNIC-TOC time-activity curves in each organ, to adjust a biokinetic model using the SAAM software, and to calculate the total number of disintegrations (N) that occurred in the source regions. N data were the input for the OLINDA/EXM code to calculate internal radiation dose estimates. Images showed an average tumor/blood (heart) ratio of 4.3{+-}0.7 in receptor-positive tumors at 1 h. The mean radiation absorbed dose calculated for a study using 740 MBq was 24, 21.5, 5.5 and 1.0 mSv for spleen, kidneys, liver and bone marrow respectively and the effective dose was 4.4 mSv.

  11. Biokinetics and dosimetry in patients of 99mTc-HYNIC-Lys3-Bombesin: images of GRP receptors

    International Nuclear Information System (INIS)

    The bombesin (BN) receptor subtype 2 (GRP-r) is expressed in several normal human tissues and is over-expressed in various human tumors including breast, prostate, small cell lung and pancreatic cancer. Recently [99mTc]EDDA/HYNIC-Lys3-bombesin (99mTc-HYNIC-BN) was reported as a radiopharmaceutical with high stability in human serum, specific cell GRP-r binding and rapid cell internalization. The aim of this study was to evaluate the feasibility of using 99mTc-HYNIC-BN to image GRP-r and to assess the radiopharmaceutical biokinetics and dosimetry in 4 breast cancer patients and in 7 healthy women. Methods: Whole-body images were acquired at 20, 90, 180 min and 24 h after 99mTc-HYNIC-BN administration. Regions of interest (ROIs) were drawn around source organs on each time frame. The same set of ROIs was used for all 11 scans and the cpm of each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate 99mTc-HYNIC-BN time-activity curves in each organ in order to calculate the total number of disintegrations (N) that occurred in the source regions, according with MIRD methodology. N data were the input for the OLINDA/EXM code to calculate internal radiation dose estimates. Results: Images showed a rapid radiopharmaceutical blood clearance with renal excretion as predominant route. 99mTc-HYNIC-BN exhibited high in vivo affinity for GRP-r over-expression successfully visualized in cancer mammary glands and well differentiated from the ubiquitous GRP-r expression in normal breast, lungs and airways. There was no statistically significant difference (p > 0.05) in the radiation absorbed doses between cancer patients and healthy women. The average equivalent doses (n=11) for a study using 740 MBq were 24.8 +- 8.8 mSv (kidneys), 7.3 +- 1.8 mSv (lungs), 6.5 +- 4.0 mSv (breast) 2.0 +- 0.3 mSv (pancreas), 1.6 +- 0.3 mSv (liver), 1.2 +- 0.2 mSv (ovaries) and 1.0 +- 0.2 mSv (red marrow). The mean effective dose was 3

  12. Water-pipe smoking effects on pulmonary permeability using technetium-99m DTPA inhalation scintigraphy

    International Nuclear Information System (INIS)

    Although extensive work has been done on cigarette smoking and its effects on pulmonary function, there are limited number of studies on water-pipe smoking. The effects of water-pipe smoking on health are not widely investigated. The aim of this study was to determine the effects of water-pipe smoking on pulmonary permeability. Technetium-99m DTPA inhalation scintigraphy was performed on 14 water-pipe smoker volunteers (all men, mean age 53.7±9.8) and 11 passive smoker volunteers (1 woman, 10 men, mean age 43.8±12). Clearance half-time (T 1/2) was calculated by placing a monoexponential fit on the time activity curves. Penetration index (PI) of the radioaerosol was also calculated. PI was 0.58±0.14 and 0.50±0.12 for water-pipe smokers (WPS) and passive smokers (PS) respectively. T 1/2 of peripheral lung was 57.3±12.7 and 64.6±13.2 min, central airways was 55.8±23.5 and 80.1±35.2 min for WPS and PS, respectively (p≤0.05). Forced expiratory volume in one second/forced vital capacity (FEV1/FVC)% was 82.1±8.5 (%) and 87.7±6.5 (%) for WPS and PS, respectively (0.025< p≤0.05). We suggest that water-pipe smoking effects pulmonary epithelial permeability more than passive smoking. Increased central mucociliary clearance in water-pipe smoking may be due to preserved humidity of the airway tracts. (author)

  13. Tc-99m p-aminohippuric acid renal scintigraphy: results of preliminary animal and human studies

    International Nuclear Information System (INIS)

    Purpose: Tc-99m p-aminohippuric acid (PAH) is a new radiopharmaceutical labeled with Tc-99m in presence of calcium three sodium salt of DTPA by use of Sn(II) - reduction method. Tc-99m PAH is rapidly secreted by the kidneys in a manner consistent with tubular secretion, as confirmed by rat probenecid studies. The present study aimed at evaluating Tc-99m PAH as renal imaging agent. Methods: After bolus injection of radiopharmaceutical in a Wister rat (17 MBq) and in 10 healthy subjects - 2 normal volunteers and 8 potential kidney. donors (111 MBq) dynamic images were acquired for 20 min in three frame groups. In all patients Tc-99m MAG3 and Tc-99m DTPA studies were also performed several days later, in l day intervals to acquire renal studies for comparison. The scintigraphic images and time/activity curves were analyzed. Results: Rat study gave typical three-phase renogram and satisfactory renal functional images with no significant extrarenal background. During Tc-99m PAH scintigraphy in humans rapid disappearance of radiopharmaceutical from the vascular system and high uptake in kidneys followed by its very fast elimination and low residual activity at the end of the study were registered. Anterior whole body images obtained at 40 min and 3 h post injection to evaluate the distribution of 99mTc-PAH within the body showed neither gallbladder nor liver activity. Mean values of the following semi-quantitative parameters were calculated; time to peak (Tmax): 3.65±0.91, time from peak to 50% of peak activity (T1/2): 6.91±2.71, percentage of residual activity in each kidney at the end of the study: 26±2.71 and split renal function: 51.26±3.92. Mean values of Tmax and T1/2 obtained with Tc-99m PAH were significantly lower in comparison with Tc-99m DTPA. Mean values of relative function did not show any significant difference between three tracers. Conclusion: Our preliminary results confirmed good biological characteristics of 99mTc-PAH, indicating its potential

  14. Evaluation of myocardial perfusion using Tc-99m MIBI

    International Nuclear Information System (INIS)

    Tc-99m MIBI (methoxy isobutyl isonitrile) is newly introduced cationic myocardial perfusion agent. For its favorable dosimetry, upto 30 mCi of Tc-99m MIBI can be used in a patient. Therefore, unlike T1-201, first-pass ejection fraction, ventricular wall motion, and SPECT studies are readily evaluated using Tc-99m MIBI on patients who are undergoing myocardial perfusion study. Nineteen individuals entered into this study and 9 of 19 had T1-201 myocardial scintiscan. For the detection of myocardial ischemia, an IV dipyridamole with hand-grip or bicycle exercise was used. Tc-99m MIBI scintigraphic findings are compared to patients 'EKG' T1-201 scans and coronary angiograms. The result were summerized as follows; 1. In 9 cases, Tc-99m MIBI studies were comparable to T1-201 scintiscans. 2. In the remaining 10 cases, Tc-99m MIBI images were correlated well with clinical pictures, EKG, and angiographic findings. 3. Using Tc-99m MIBI, one can easily obtain fist-pass ejection fraction, ventricular wall motion and SPECT studies. It was concluded that Tc-99m MIBI seems to be an excellent agent for myocardial perfusion study. Tc-99m MIBI images are superior in quality than T1-201 images mainly due to high photon yield and ideal energy of Tc-99m MIBI

  15. Retention of 99mTc-DMSA(III) and 99mTc-nanocolloid in different syringes affects imaging quality.

    Science.gov (United States)

    Bauwens, Matthias; Pooters, Ivo; van der Pol, Jochen; Mottaghy, Felix M; van Kroonenburgh, Marinus

    2014-04-01

    (99m)Tc-dimercaptosuccinic acid [DMSA(III)] and colloidal human serum albumin ((99m)Tc-nanocolloid) are widely used radiopharmaceuticals. Recently, in our institution we encountered image quality problems in DMSA scans after changing the brand of syringes we were using, which triggered us to look into the adsorption properties of syringes from different brands for (99m)Tc-DMSA(III) and (99m)Tc-nanocolloid. We also describe a clinical case in which adsorption of (99m)Tc-DMSA(III) caused inferior imaging quality. DMSA and nanocolloid were labeled with (99m)Tc following manufacturer guidelines. After synthesis, syringes with (99m)Tc-DMSA(III) and (99m)Tc-nanocolloid were stored for 15, 30, 60, and 120 min. We evaluated Luer Lock syringes manufactured by different brands such as Artsana, Henke-Sass-Wolf, B. Braun Medical N.V., CODAN Medizinische Geräte GmbH & Co KG, Becton Dickinson and Company, and Terumo Europe. Adsorption of (99m)Tc-DMSA(III) and (99m)Tc-nanocolloid was acceptably low for all syringes (brands with (99m)Tc-DMSA(III) adsorption rates of 36 and 30%, respectively, and for one brand with a (99m)Tc-nanocolloid adsorption rate of 27%. Adsorption of (99m)Tc-DMSA(III) and (99m)Tc-nanocolloid reaches critical levels in syringes produced by two brands, potentially causing poor image quality--for example, in DMSA scans using pediatric radiopharmaceutical doses. It is advised to check the compatibility of any radiopharmaceutical with syringes as an integral part of the quality assurance program. PMID:24569706

  16. Sup(99m)Tc compounds for diagnostic purposes

    International Nuclear Information System (INIS)

    The applications of sup(99m)Tc in nuclear medicine are discussed, such as sodium pertechnetate in thyroid and brain scintigraphy, complex compounds in lungs and liver diao.nosis. Technetium generators are classified according to the method of separating sup(99m)Tc from 99Mo. Adsorption generators are used, molybdate-99 is adsorbed on an Al2O3-packed column while pertechnetate-99m is eluted with 0.9% NaCl solution. Also used is continuous pertechnetate-99m extraction with methyl ethyl ketone from 0.5 M potassium molybdate and 2.5 M of potassium carbonate. The manufacture is described of kits for sup(99m)Tc radiopharmaceuticals preparation, eg., Diagos I, a gluconate complex, a lyophilisate for sup(99m)Tc-sodium pyrophosphate injections, a diagnostic kit for lung scintiscanning. (H.S.)

  17. Biokinetics and dosimetry of {sup 99m} Tc-EDDA/HYNIC-[Lys{sup 3}]-bombesin in humans: imaging of GRP receptors

    Energy Technology Data Exchange (ETDEWEB)

    Santos C, C.L.; Ferro F, G. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico); Murphy, C.A de [INCMNSZ, 14000 Mexico D.F. (Mexico); Cardena, E.; Pichardo R, P. [Departamento de Medicina Nuclear, Oncologia Centro Medico Siglo XXI, Mexico D.F. (Mexico)

    2007-07-01

    Full text: Bombesin (BN) receptor subtype 2 (GRP-r) is over-expressed on various human tumors including breast, prostate, small cell lung and pancreatic cancer. Recently we reported the {sup 99-}mTc-EDDA/HYNIC-[Lys{sup 3}]-Bombesin ({sup 99m}Tc-HYNIC-BN) complex as a new radiopharmaceutical with high stability in human serum, specific cell GRP-receptor binding and rapid internalization. The aim of this study was to evaluate the {sup 99m}Tc-HYNIC-BN biokinetics and dosimetry in 5-healthy and 3-breast cancer women. Whole-body images were acquired at 20, 90, 180 min and 24 h after {sup 99m}Tc-HYNIC-BN administration. Regions of interest (ROIs) were drawn around source' organs on each time frame. The same set of ROIs was used for all 8 scans and the cpm of each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate {sup 99m}Tc-HYNIC-BN time activity curves in each organ, to calculate the total number of disintegrations (N) that occurred in the source regions. N data were the input for the OLINDA/EXM code to calculate internal radiation dose estimates. Images showed a rapid radiopharmaceutical blood clearance with predominantly renal excretion and minimal hepatobiliary elimination. {sup 99m}Tc-HYNIC-BN exhibited high in vivo affinity for GRP-r over-expression successfully visualized in breast cancer lesions and well differentiated from GRP-r expression in lungs and airways with normal GRP-r density (ratio 3:1). The equivalent doses for a study using 370 MBq were 7.38{+-}1.68, 0.59{+-}0.08, 2.07{+-}0.60, 0.58{+-}0.1, 0.75{+-}0.09 and 0.43{+-}0.07 mSv for kidneys, liver, lungs, ovaries, pancreas and red marrow respectively. The effective dose was 1.64{+-}0.25 mSv which is comparable with the doses known for most of the {sup 99m}Tc radiopharmaceutical studies in nuclear medicine. (Author)

  18. {sup 99m}Tc-interleukin-2 and {sup 99m}Tc-HMPAO granulocyte scintigraphy in patients with inactive Crohn's disease

    Energy Technology Data Exchange (ETDEWEB)

    Annovazzi, Alessio; Chianelli, Marco; Capriotti, Gabriela; Signore, Alberto [Nuclear Medicine Unit, Department of Clinical Sciences, Policlinico Umberto I, University ' ' La Sapienza' ' , 00161, Rome (Italy); Biancone, Livia; Pallone, Francesco [Gastroenterology Unit, University ' ' Tor Vergata' ' , Rome (Italy); Caviglia, Renato [Gastroenterology Unit, University ' ' Campus Biomedico' ' , Rome (Italy); Mather, Stephen J. [Department of Nuclear Medicine, St Bartholomew' s Hospital, Cancer Research UK, London (United Kingdom); Caprilli, Renzo [Gastroenterology Unit, Department of Clinical Sciences, University ' ' La Sapienza' ' , Rome (Italy); Scopinaro, Francesco [Department of Radiological Sciences, University ' ' La Sapienza' ' , Rome (Italy)

    2003-03-01

    .91, respectively) but a weak positive predictive value (0.44 and 0.39, respectively). Nevertheless, Kaplan-Meier curves generated between scintigraphic findings and time free from disease relapse were statistically different only for {sup 99m}Tc-IL2 scintigraphy (log-rank test, P=0.013). These results indicate that {sup 99m}Tc-IL2 scintigraphy can be useful in selecting CD patients in clinical remission who could benefit from preventive therapy to avoid disease relapse. (orig.)

  19. Standardization of a method to calculate absolute renal uptake of {sup 99m} Tc-DMSA in children; Padronizacao do metodo para calculo da captacao renal absoluta do {sup 99m}Tc-DMSA em criancas

    Energy Technology Data Exchange (ETDEWEB)

    Ono, Carla Rachel; Sapienza, Marcelo Tatit; Watanabe, Tomoco; Costa, Paulo Luiz Aguirre; Okamoto, Miriam Roseli Yoshie; Garcez, Alexandre Teles; Buchpiguel, Carlos Alberto [Sao Paulo Univ., SP (Brazil). Hospital das Clinicas. Servico de Medicina Nuclear]. E-mail: crachelo@usp.br; Machado, Beatriz Marcondes; Machado, Marcia Melo Campos [Paulo Univ., SP (Brazil). Faculdade de Medicina. Hospital Universitario; Liberato Junior, Waldyr de Paula

    2006-01-15

    Objective:To standardize a method and determine normal values for absolute renal uptake of 99m Tc-DMSA in children with normal creatinine clearance. Materials and methods: Twenty-two children (between 7 months and 10 years of age; mean 4.5 years) without clinical evidence of renal disease were studied using 99m Tc-DMSA scintigraphy. Eighteen had normal renal ultrasonography, micturating urethrocystography, creatinine clearance and visual interpretation of the scintigraphy with 99m Tc-DMSA. Four children were excluded, one with incomplete creatinine clearance and three due to reduction in the creatinine clearance. Absolute renal uptake of 99m Tc-DMSA (DMSA-Abs) was expressed as the fraction of the administered dose retained by each kidney six hours after administration of the radiopharmaceutical. Results: DMSA-Abs was 21.8 +- 3.2% for the right kidney and 23.1 +-3.3% for the left kidney. There was no correlation between renal uptake and the age groups studied, although there was a tendency to an increase in the creatinine clearance with age. Conclusion: Normal values of DMSA-Abs can be used as an additional parameter for the initial diagnostic evaluation and during follow-up of renal diseases, mainly when bilateral impairment of renal function is suspected or in a patient with a single functioning kidney (in which renal differential function is of limited value). (author)

  20. 99mTc-ciprofloxacin for diagnosis of bacterial infection

    Science.gov (United States)

    Aungurarat, A.; Ngamprayad, T.; Dangprasert, M.; Phumkem, S.; Jowanaridhi, B.

    2015-05-01

    Preparation of 99mTc-ciprofloxacin for diagnosis of bacterial infection was investigated by varying factors which affected this compound. The optimum conditions for preparation of 99mTc-ciprofloxacin and a lyophilized kit for Tc-99m labelling were studied. The results from biodistribution study showed that the percentages of the injected dose per gram tissues of infected area at 1 and 3 hours after injection were around 0.25-0.56. 99mTc-ciprofloxacin was found sterile, pyrogen-free and non-toxic. Radiochemical purity was greater than 90% with greater than 6 hours of stability.

  1. Imaging bone infection using Tc-99m infecton: case studies

    International Nuclear Information System (INIS)

    Tc-99m infecton (ciprofloxacin) was introduced recently as an infection imaging agent which is specific for bacteria. If successful, this may prove superior to labelled leucocyte imaging, antibodies and other nonspecific agents in terms of logistics, cost and efficacy. We had recently acquired few kits for Tc-99m infecton preparation. Our preliminary experience suggests that it has high specificity for the infected bone and may be useful in bone tuberculosis. Three cases are presented where both Tc-99m MDP scanning and Tc-99m infecton study were undertaken to highlight the potential of this new agent. (author)

  2. Development and evaluation of a single vial cold kit for infection imaging: Tc-99m Ciprofloxacin

    International Nuclear Information System (INIS)

    Radiolabeled antibiotics have been used in nuclear medicine for the diagnosis of bacterial infection. They are used as specific infection-imaging agents because of their affinity to bind with bacteria. Tc-99m Ciprofloxacin is the most widely used infection-imaging agent. The aim of the present work is to increase the shelf life of an in house developed single vial kit for the preparation of Tc-99m Ciprofloxacin. Different methods were used to make the Ciprofloxacin kits. Kits were stored at a temperature of 4 deg. C under nitrogen atmosphere and evaluated for stability by serially estimating the labeling efficiency using instant thin layer chromatography (ITLC) following reconstitution with Tc99m pertechnetate at regular intervals of time up to 90 days after their preparation. The data were subjected to Sigmaplotsoftware version 8.0 for shelf-life analysis. The kits were further evaluated for a number of standard quality control parameters. Kits containing sodium chloride-Ciprofloxacin pellets were found to have a shelf life of 7 months and constantly maintaining a labeling efficiency of 95% Tc-99m Ciprofloxacin. Biodistribution studies in balb/c mice showed significant levels in kidney, liver and intestine. Blood clearance studies showed a slow and biphasic clearance in rabbits. Gamma camera imaging of the rabbits bearing S.aureus infectious lesion confirmed the utility and specificity of the reconstituted kit in imaging infection. The final kit, which was recommended for clinical use was found to be stable for nine months with all its characteristics remaining unchanged from those of the freshly prepared Tc-99m Ciprofloxacin during the entire period of observation. (author)

  3. The radiopharmaceuticals labelled with technetium-99m and the radiopharmacy; Les radiopharmaceutiques marques au technetium-99m et la radiopharmacie

    Energy Technology Data Exchange (ETDEWEB)

    Bodenant, V

    1998-10-01

    In less than fifty years, the place of nuclear medicine is become primordial. Among all the radiopharmaceuticals used in nuclear medicine, the technetium-99m is the most used because of its physico-chemical properties and its great availability with the molybdenum-99m - technetium-99m generator. Since 1992, the radiopharmaceuticals, the packages, the generators are included in the pharmaceutic monopole. They are now under the reliability of the radio-pharmacist. This thesis has for object to introduce these different radiopharmaceuticals labelled with technetium-99m and to show the primordial place of the radio-pharmacist in a service of nuclear medicine. (N.C.)

  4. Comparative scintigraphic study of renal function with 99mTc-Mercaptoacetyl-triglycine and 99mTc-Ethylenedicysteine

    OpenAIRE

    Beiki D.; Eftekhari M; Fallahi B.; Gheisari F.; Hozhabrosadati M.; Fard-Esfahani A.; Takavar A; Gholamrezanezhad A.; Saghari M. Ansari Gilani K.

    2008-01-01

    99mTc-MAG3 is a standard radiotracer for renal dynamic functional study. Despite its"nproperties for clinical uses, it has numerous technical limitations. 99mTc-EC is also a tubular"nradiotracer for renal imaging, which has not been used worldwide. In this study, the use of 99mTc-EC"nand 99mTc-MAG3 for renal functional study were compared."nMethods: Thirty five patients (20 male, 15 female; mean age of 34.63 ±10.69 years) were entered in"nthe study. About 1...

  5. Sustained availability of 99mTc: possible paths forward.

    Science.gov (United States)

    Pillai, Maroor Raghavan Ambikalmajan; Dash, Ashutosh; Knapp, F F Russ

    2013-02-01

    The availability of (99m)Tc for single-photon imaging in diagnostic nuclear medicine is crucial, and current availability is based on the (99)Mo/(99m)Tc generator fabricated from fission-based molybdenum (F (99)Mo) produced using high enriched uranium (HEU) targets. Because of risks related to nuclear material proliferation, the use of HEU targets is being phased out and alternative strategies for production of both (99)Mo and (99m)Tc are being evaluated intensely. There are evidently no plans for replacement of the limited number of reactors that have primarily provided most of the (99)Mo. The uninterrupted, dependable availability of (99m)Tc is a crucial issue. For these reasons, new options being pursued include both reactor- and accelerator-based strategies to sustain the continued availability of (99m)Tc without the use of HEU. In this paper, the scientific and economic issues for transitioning from HEU to non-HEU are also discussed. In addition, the comparative advantages, disadvantages, technical challenges, present status, future prospects, security concerns, economic viability, and regulatory obstacles are reviewed. The international actions in progress toward evolving possible alternative strategies to produce (99)Mo or (99m)Tc are analyzed as well. The breadth of technologies and new strategies under development to provide (99)Mo and (99m)Tc reflects both the broad interest in and the importance of the pivotal role of (99m)Tc in diagnostic nuclear medicine.

  6. Quality control of techetium 99m radiopharmacentical in nuclear medicine

    International Nuclear Information System (INIS)

    Gel chromatography column scanning (GCS) is a new method for radiochemical quality control. GCS techniques for Technetium-99m radiopharmaceuticals in nuclear medicine have been developed for use in both research and routine clinical work. The dependence on several of the parameters of the GCS method have been investigated, e.g. type of gel, column dimensions, eluent, equilibration, elution volume, flow rate and resolution of the recording system (radiochromatographic scanner or scintillation camera). The GCS method has been compared with conventional gel filtration, thinlayer cromatography (TLC) and paper cromatography (PC). The GCS method is to be preferred due to few artifacts, much information, good reproducibility, rapidity, simplicity and the convenience of the test. The GCS method has been applied to the development of labelling techniques for the new radiopharmaceuticals Tc-99m plasmin and Tc-99m unithiol (2.3 dimercaptopropane sodiumsulphonate), use for investigating deep vein thrombosis and renal cortical morphology respectively. The GCS method has also been applied for studying some labelling parameters, the radiochemical purity and the labelling of Tc-99m macroaggregated albumin, Tc-99m pyrophosphate, Tc-99m methylenedisphosphate, in addition to Tc-99m plasmin and Tc-99m unithiol. (Author)

  7. Muscle localization of Tc-99m MDP after exertion

    Energy Technology Data Exchange (ETDEWEB)

    Valk, P.

    1984-09-01

    Very high muscle uptake of Tc-99m MDP was seen two days after the start of a program of vigorous weight-lifting exercises. Localization of Tc-99m bone tracer in muscle that has been damaged by exertion may be a more common phenomenon than is recognized at present.

  8. 99mTc-labelled biguanide derivatives: chemical speciation modelling thereof and evaluation in vervets

    International Nuclear Information System (INIS)

    99mTc-DMSA (dimercaptosuccinic acid) is known to be a safe and effective agent for static renal imaging. However, it has a long uptake time which is a limiting factor in diagnostic procedures and also leads to a relatively high radiation dose being administered to patients. There is a constant search for possible new renal imaging agents with a good resolution, kidney/liver contrast and low radiation dose to all organs. A series of biguanide derivatives (potential as non-insulin-dependent diabetes mellitus agents) labelled with 99mTc were investigated as potential alternative kidney-imaging agents on theoretical grounds (in silico) and their biodistribution (in vivo) verified in a limited number of animal experiments. Such a dual approach has the benefit that it reduces the number of animal experiments needed to evaluate a potential radiopharmaceutical. The blood plasma model shows little or no complexation of the biguanide type ligands by the metal ions in blood plasma. It was therefore expected that these ligands will clear rapidly through the kidneys and liver (increased lipophilicity). These predictions were verified by studies on single vervets comparing them with 99mTc-DMSA as gold standard. All the biguanide derivatives labelled with 99mTc show liver, kidney and gallbladder uptake in vervets. It was shown that the agent 99mTc-CBIG (carboxylbiguanide) has a very fast kidney clearance, which will reduce the dose to organs (as experienced for 99mTc-DMSA), although it's potential as a kidney agent is limited by its gallbladder uptake. (author)

  9. Different dynamic of Tc-99m-tetrofosmin and Tc-99m-MIBI in a parathyroid adenoma; Unterschiedliche Dynamik von Tc-99m-Tetrofosmin und Tc-99m-MIBI in einem Nebenschilddruesen-Adenom

    Energy Technology Data Exchange (ETDEWEB)

    Nowak, B.; Sabri, O.; Reinartz, P.; Kleinhans, E.; Zimny, M. [Universitaetsklinikum Aachen (Germany). Klinik fuer Nuklearmedizin; Hoff, C. [Universitaetsklinikum Aachen (Germany). Medizinische Klinik III

    1999-07-01

    Case report of a patient with hypercalcemia and suspected parathyroid adenoma. Because of a previous strumectomy a precise preoperative localization of the suspected parathyroid adenoma was demanded. Tc-99m-tetrofosmin scintigraphy in double phase technique failed to detect a parathyroid adenoma by failing to show a region of increased focal uptake with delayed washout in relation to the thyroid gland. Only comparison of the Tc-99m-tetrofosmin images with a Tc-99m-pertechnetate scan revealed a right caudal parathyroid adenoma. A double phase Tc-99m-MIBI study of the same patient was able to localize this parathyroid adenoma without the need of a corresponding Tc-99m-pertechnetate scintigraphy due to a differential washout with persistent focal uptake in the parathyroid adenoma and a progressively decreasing uptake in the thyroid tissue. This case indicates that Tc-99m-tetrofosmin is a suitable agent for parathyroid imaging only if used together with Tc-99m-pertechnetate but is seems to lack the differential washout characteristics of Tc-99m-MIBI according parathyroid gland and thyroid gland. (orig.) [German] Bei einer Patientin mit Hyperkalzaemie und Verdacht auf primaeren Hyperparathyreoidismus wurde wegen einer vorherigen Strumektomie eine genaue praeopertive Lokalisationsdiagnostik gefordert. Eine in Zwei-Phasen-Technik durchgefuehrte Szintigraphie mit Tc-99m-Tetrofosmin allein erbrachte keinen sicheren Nachweis eines Nebenschilddruesen-Adenoms, da an keiner Lokalisation eine fokale Mehranreicherung mit relativ zur Schilddruese verzoegertem Washout festzustellen war. Erst der Vergleich mit dem Tc-99m-Pertechnetat-Szintigramm ergab den Hinweis auf ein Nebenschilddruesen-Adenom rechts kaudal. Eine zusaetzlich durchgefuehrte Tc-99m-MIBI-Szintigraphie konnte dieses Adenom alleine aufgrund einer hier vorhandenen Persistenz der Nuklidspeicherung im Nebenschilddruesen-Adenom bei gleichzeitig deutlichem Washout aus der uebrigen Schilddruese nachweisen. Dies weist darauf

  10. Technetium-99m sestamibi kinetics in reperfused canine myocardium

    International Nuclear Information System (INIS)

    The purpose of the current study was to clarify the myocardial kinetics of technetium-99m sestamibi when the latter is administered during reperfusion. Sestamibi has in the past been given to patients following thrombolytic therapy to document reperfusion and assess salvage. However, the factors which affect sestamibi kinetics during reperfusion are not clearly defined. In this study the left circumflex coronary artery was occluded for 2 h in six dogs (group 1) and for 3 h in six dogs (group 2), followed by reperfusion. Five additional dogs were not reperfused (group 3). Sestamibi was administered during reperfusion in groups 1 and 2, and during ongoing occlusion in group 3. Regional myocardial sestamibi activity was monitored for 3 h using miniature implanted radiation detectors and gamma camera imaging. Group 1 dogs had no infarcts, group 2 had moderate infarcts (mean: 13.9%), and group 3 had large infarcts (mean: 25.2%). Three-hour fractional myocardial clearances were significantly greater for reperfused infarcted (group 2) (0.23±0.02 SEM) and for nonreperfused infarcted myocardium (group 3) (0.24±0.02) compared to control (0.10±0.01) and reperfused noninfarcted myocardium (group 1) (0.07±0.02; P<0.01). Quantitative image analysis demonstrated a significant reduction in the left circumflex/left anterior descending count ratios from initial to final scans for group 2 (0.74±0.03 to 0.65±0.03, P<0.05), and a trend towards a reduction in the count ratios from initial to final scans for group 3 (0.38±0.04 to 0.30±0.04; P=0.06). Thus, probederived myocardial sestamibi kinetics following reperfusion do differentiate non-infarcted from infarcted myocardium. Although the detection of accelerated clearance can be demonstrated by quantitative analysis of gamma camera images in dogs, this may be technically difficult in clinical situations. (orig.)

  11. Initial experience with 99mTc-tetrofosmin scintimammography

    International Nuclear Information System (INIS)

    Full text:99mTc-tetrofosmin prone scintimammography was performed in 12 patients with palpable breast lesions and abnormal mammography findings. Right and left lateral prone and anterior supine views were obtained starting at 10 min of intravenous injection of 20 mCi 99mTc labelled tetrofosmin. Focal increased concentration of radiopharmaceutical as compared to surrounding normal breast tissue was considered positive for malignant disease. Patients underwent surgery with histopathological examination. 7 of the eight malignant lesions showed accumulation of 99mTc-tetrofosmin. Involved lymph nodes were also seen to concentrate the radiopharmaceutical. Four benign breast masses did not show focal 99mTc-tetrofosmin uptake. Initial results suggest that 99mTc-tetrofosmin could play a useful role in evaluation of breast tumours

  12. Comparative scintigraphic study of renal function with 99mTc-Mercaptoacetyl-triglycine and 99mTc-Ethylenedicysteine

    Directory of Open Access Journals (Sweden)

    Beiki D.

    2008-03-01

    Full Text Available 99mTc-MAG3 is a standard radiotracer for renal dynamic functional study. Despite its"nproperties for clinical uses, it has numerous technical limitations. 99mTc-EC is also a tubular"nradiotracer for renal imaging, which has not been used worldwide. In this study, the use of 99mTc-EC"nand 99mTc-MAG3 for renal functional study were compared."nMethods: Thirty five patients (20 male, 15 female; mean age of 34.63 ±10.69 years were entered in"nthe study. About 10 mCi of 99mTc-EC and 99mTc-MAG3 were administered in different days within 7"ndays intervals and serial images were obtained for 30 minutes. Serum creatinine and visual"nscintigraphic findings of all patients were within normal limits."nResults: In this study, the renal uptake of 99mTc-EC was significantly higher than 99mTc-MAG3 (6.20%"nvs 4.39% of the injected dose, while the hepatic activity of 99mTc-EC was significantly lower (307 vs"n439 mean pixel count, p<0.0001. Also the absolute values for some other quantitative parameters"nsuch as right and left kidney transit times and ERPF were different for each radiotracer. In spite of"nthese differences most quantitative parameters (except for right kidney transit time, liver uptake and"nTmax of both kidneys showed good correlations for both agents."nConclusion: The calculated ERPF with 99mTc-EC as compared to 99mTc-MAG3 is in closer proximity"nto the true values. Also the use of 99mTc-EC compared to 99mTc-MAG3 in clinic due to simplicity of"npreparation, higher stability, higher renal uptake, and lower hepatobiliary activity is better. Therefore"nthe use of 99mTc-EC as an appropriate substitute or ever preferred radiopharmaceutical for 99mTc-"nMAG3 radiopharmaceutical for renal function studies is recommended.

  13. A Comparative Study of 99mTc-YIGSR and 99mTc-MIBI Uptake in Tumor Cells

    Institute of Scientific and Technical Information of China (English)

    HU Jia; LAN Xiaoli; ZHANG Yongxue; GAO Zairong; WU Jun

    2005-01-01

    To investigate a new kind of tumor tracer 99mTc-YIGSR developed from a five amino structure (YIGSR) of the Laminin -chain,which can bind to the laminin receptors of tumor specifically, and radiolabeled with MAG3. (1) Preparation of the 99m Tc-YIGSR probe: with S-Acetly-NH3-MAG3as the chelator and with proper reductants YIGSR was labeled with 99mTc; (2) Cell culture and viability measurement: EAC was maintained in RPMI 1640 supplemented with calf serum; the trypan blue exclusion was applied to calculate the cell viability; (3) Study of the cell dynamic: The EACs uptake of 99mTc-YIGSR and99mTc-MIBI was observed at 37 ℃ and 22 ℃, respectively. (1)The labeling efficiencies of 99mTc-YIGSR and99mTc-MIBI were (62±3) % and (96±2) %, respectively; (2) The cell viability was declined with time of incubation; (3) At 37 ℃, the EACS uptake of 99mTc-YIGSR and99mTc-MIBI reached the peak of (43. 16±2.4) % and (24.4±1.8) % at 60min, respectively; and at 22 ℃, the highest uptake was (26.5±2.1) % and (9. 47±1.9) % at 60min, respectively. The in vitro study suggests that 99mTc-YIGSR is superior to 99mTc-MIBI in cell uptake and has potential value in tumor imaging.

  14. Comparison of uptake of 99mTc-MIBI, 99mTc-tetrofosmin and 99mT-Q12 into human breast cancer cell lines

    International Nuclear Information System (INIS)

    Technetium-99m hexakis-2-methoxyisobutyl-isonitrile (MIBI), 99mTc-tetrofosmin and 99mTc-Q12 were all introduced for myocardial imaging but found additional applications as they are taken up by different tumours, enabling imaging of these lesions in patients. The aim of this study was to compare the uptake characteristics of these compounds in vitro in the human adenocarcinoma breast cell lines MCF-7 and ZR-75. It was shown that 99mTc-MIBI had the highest cellular uptake (15.9%±0.5% dose/mg protein after 60 min in MCF-7, and 14.2%±0.4% dose/mg protein in ZR-75), followed by 99mTc-tetrofosmin (6.8%±0.6% dose/mg protein in MCF-7, and 8.2%±0.2% dose/mg protein in ZR-75) and 99mTC-Q12 (3.2%±0.1% dose/mg protein in MCF-7, and 3.5%±0.3% dose/mg protein in ZR-75 cells). For all three compounds tenfold differences in specific activity did not influence total cell-associated radioactivity. Uptake of 99mTc-MIBI and 99mTc-tetrofosmin was obviously lower at 4 C than at 37 C, whereas 99mTc-Q12 uptake showed only slight temperature dependence. When uptake was compared in cells grown to different cell densities (1 mg/ml cellular protein versus 0.3 mg/ml), no differences in uptake were detected when uptake was corrected for the amount of cellular protein present in the dishes. Furthermore, for all compounds it was shown that cellular radioactivity decreased rapidly after washing. Apart from the differences in cellular uptake of the three compounds after 60 min, no differences in residual cellular radioactivity after washing were found between the different compounds when expressed as a percentage of their 60-min uptake, suggesting that the efflux process of the radiolabelled compounds was similar. The differences in cell-associated activity after 60 min were thus presumably caused by differences in uptake. (orig./MG)

  15. Evaluation of the absorbed dose to the kidneys due to Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3) and Tc{sup 99m} (Dmsa); Evaluacion de la dosis absorbida en los rinones debido al Tc{sup 99m} (DTPA) / Tc{sup 99m} (MAG3) y Tc{sup 99m} (DMSA)

    Energy Technology Data Exchange (ETDEWEB)

    Vasquez A, M.; Murillo C, F.; Castillo D, C.; Rocha J, J.; Sifuentes D, Y.; Sanchez S, P. [Universidad Nacional de Trujillo, Av. Juan Pablo II s/n, Trujillo (Peru); Idrogo C, J.; Marquez P, F., E-mail: marvva@hotmail.com [Instituto Nacional de Enfermedades Neoplasicas, Av. Angamos 2520, Lima (Peru)

    2015-10-15

    The absorbed dose in the kidneys of adult patients has been assessed using the biokinetics of radiopharmaceuticals containing Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3) or Tc{sup 99m} (Dmsa).The absorbed dose was calculated using the formalism MIRD and the Cristy-Eckerman representation for the kidneys. The absorbed dose to the kidneys due to Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3), are given by 0.00466 mGy.MBq{sup -1} / 0.00339 mGy.MBq{sup -1}. Approximately 21.2% of the absorbed dose is due to the bladder (content) and the remaining tissue, included in biokinetics of Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3). The absorbed dose to the kidneys due to Tc{sup 99m} (Dmsa) is 0.17881 mGy.MBq{sup -1}. Here, 1.7% of the absorbed dose is due to the bladder, spleen, liver and the remaining tissue, included in biokinetics of Tc{sup 99m} (Dmsa). (Author)

  16. Study on the preparation of methylene diphosphonate (MDP) labeling with 99mTc for bone scintigraphy

    International Nuclear Information System (INIS)

    The 99mTc-MDP kit was prepared by using aseptic technique. The composition of MDP invivo kit including 5 mg MDP, 0.5 mg stannous chloride, 0.5 mg ascorbic acid and 5 mg sodium chloride. The kit produces 99mTc-MDP with more than 95% labelling efficiency at pH 6-7 when mixed with requisite amount of 99mTc-pertechnetate. The preparation has been found sterity, pyrogen free, to be sufficiency stable invitro as well as invivo, blood clearance. After intravenous administrations abound 50% of the dose is accumulated and retained by the skeleton. The kit provides quality product in high yield, more stable than other phosphonate kit, high bone uptake. High avidity of 99mTc-MDP for bone imaging makes it a promising agent for the detection of bone diseases. (author)

  17. Different dynamic of Tc-99m-tetrofosmin and Tc-99m-MIBI in a parathyroid adenoma

    International Nuclear Information System (INIS)

    Case report of a patient with hypercalcemia and suspected parathyroid adenoma. Because of a previous strumectomy a precise preoperative localization of the suspected parathyroid adenoma was demanded. Tc-99m-tetrofosmin scintigraphy in double phase technique failed to detect a parathyroid adenoma by failing to show a region of increased focal uptake with delayed washout in relation to the thyroid gland. Only comparison of the Tc-99m-tetrofosmin images with a Tc-99m-pertechnetate scan revealed a right caudal parathyroid adenoma. A double phase Tc-99m-MIBI study of the same patient was able to localize this parathyroid adenoma without the need of a corresponding Tc-99m-pertechnetate scintigraphy due to a differential washout with persistent focal uptake in the parathyroid adenoma and a progressively decreasing uptake in the thyroid tissue. This case indicates that Tc-99m-tetrofosmin is a suitable agent for parathyroid imaging only if used together with Tc-99m-pertechnetate but is seems to lack the differential washout characteristics of Tc-99m-MIBI according parathyroid gland and thyroid gland. (orig.)

  18. Exploring the Potential of (99m)Tc(CO)3-Labeled Triazolyl Peptides for Tumor Diagnosis.

    Science.gov (United States)

    Gaonkar, Raghuvir H; Ganguly, Soumya; Baishya, Rinku; Dewanjee, Saikat; Sinha, Samarendu; Gupta, Amit; Ganguly, Shantanu; Debnath, Mita C

    2016-04-01

    In recent years the authors have reported on (99m)Tc(CO)3-labeled peptides that serve as carriers for biomolecules or radiopharmaceuticals to the tumors. In continuation of that work they report the synthesis of a pentapeptide (Met-Phe-Phe-Gly-His; pep-1), a hexapeptide (Met-Phe-Phe-Asp-Gly-His; pep-2), and a tetrapeptide (Asp-Gly-Arg-His; pep-3) and the attachment of 3-amino-1,2,4-triazole to the β carboxylic function of the aspartic acid unit of pep-2 and pep-3. The pharmacophores were radiolabeled in high yields with [(99m)Tc(CO)3(H2O)3](+) metal aqua ion, characterized for their stability in serum and saline, as well as in His solution, and found to be substantially stable. B16F10 cell line binding studies showed favorable uptake and internalization. In vivo behavior of the radiolabeled triazolyl peptides was assessed in mice bearing induced tumor. The (99m)Tc(CO)3-triazolyl pep-3 demonstrated rapid urinary clearance and comparatively better tumor uptake. Imaging studies showed visualization of the tumor using (99m)Tc(CO)3-triazolyl pep-3, but due to high abdominal background, low delineation occurred. Based on the results further experiments will be carried out for targeting tumor with triazolyl peptides. PMID:27093344

  19. A new method for the preparation of 99mTc-stannous colloid instant kit

    International Nuclear Information System (INIS)

    A new way for the preparation of 99mTc-stannous cooloid instant kit, its animal experimental results and its application to clinical practice were described. 500 mg sodium fluoride and 25 mg stannous chloride were dissolved in 50 ml sterile water, and the pH was adjusted to 6.4 with 0.05 mol/LHCl 0.5 millilitre of this solution was filtered into a vial and dryed under sterile condition. The kit can be used immediately after adding 2 ∼ 4 ml of 99mTcO4. It is suitable for intravenous administration as a imaging agent for liver or other organs of the reticuloerdothelial (RE) system. Distribution stadies of the 99mTc-stannous colloid in rats showed a rapid clearance of radioactivity from the blood stream and a subsequent rapid increase of radioactivity in the liver, up to approximately 90% of the injected dose. No toxic reactions were observed in rabbits at a dose of 1 ml of 99mTc-stannous colloid/kg

  20. Effects of concurrent drug therapy on technetium /sup 99m/Tc gluceptate biodistribution

    Energy Technology Data Exchange (ETDEWEB)

    Hinkle, G.H.; Basmadjian, G.P.; Peek, C.; Barker, K.K.; Ice, R.D.

    1982-11-01

    Drug interactions with /sup 99m/Tc gluceptate resulting in altered biodistribution were studied using chart review and animal tests. Charts of nine patients who had abnormal gallbladder uptake of technetium /sup 99m/Tc gluceptate during a two-year period were reviewed to obtain data such as concurrent drug therapy, primary diagnosis, and laboratory values. Adult New Zealand white rabbits were then used for testing the biodistribution of technetium /sup 99m/Tc gluceptate when administered concurrently with possibly interacting drugs identified in the chart review--penicillamine, penicillin G potassium, penicillin V potassium, acetaminophen, and trimethoprim-sulfamethoxazole. Chart review revealed no conclusive patterns of altered biodistribution associated with other factors. The data did suggest the possibility that the five drugs listed above might cause increased hepatobiliary clearance of the radiopharmaceutical. Animal tests showed that i.v. penicillamine caused substantial distribution of radioactivity into the gallbladder and small bowel. Minimally increased gallbladder radioactivity occurred when oral acetaminophen and trimethoprim-sulfamethoxazole were administered concurrently. Oral and i.v. penicillins did not increase gallbladder activity. Penicillamine may cause substantial alteration of the biodistribution of technetium /sup 99m/Tc gluceptate.

  1. Tc-99m Ciprofloxacin SPECT of Pulmonary Tuberculosis

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Min Kyung; Hwang, Kyung Hoon [Gachon University Gil Hospital, Incheon (Korea, Republic of); Yoon, Min Ki [Good Samaritan Hospital, Pohang (Korea, Republic of); Choe, Won Sick [Kangbuk Samsung Hospital, Seoul (Korea, Republic of)

    2010-06-15

    Tc-99m ciprofloxacin is available for imaging infection. However, there has been no study on employing single photon emission computed tomography (SPECT) with using Tc-99m ciprofloxacin to image active pulmonary tuberculosis. Therefore, we conducted this study to assess the efficacy of Tc-99m ciprofloxacin SPECT for imaging active pulmonary tuberculosis. Twenty-one participants were enrolled in this prospective study. They were divided into two groups according to the clinical and radiological assessment. Group one (Gr. 1) consisted of five normal volunteers and six patients with inactive pulmonary tuberculosis. Group two (Gr. 2) consisted of ten patients with active pulmonary tuberculosis. SPECT was performed 3 h after injecting 555 MBq (15 mCi) of Tc-99m ciprofloxacin. The findings of Tc-99m ciprofloxacin SPECT were interpreted by a nuclear medicine specialist and then the results were analyzed according to the patients' clinical and radiological classifications. The results of Tc-99m ciprofloxacin SPECT were as follows: eight true-positive cases, ten true-negative cases, one false-positive case and two false-negative cases. The sensitivity and specificity was 80.0% and 90.0%, respectively. The positive predictive value was 88.9% and the negative predictive value was 83.3%. Conclusions Tc-99m ciprofloxacin SPECT is feasible for imaging active pulmonary tuberculosis. It is a useful nuclear-imaging method for discriminating between the active and inactive tuberculosis states in patients with a past medical history of pulmonary tuberculosis.

  2. Tc-99m Ciprofloxacin SPECT of Pulmonary Tuberculosis

    International Nuclear Information System (INIS)

    Tc-99m ciprofloxacin is available for imaging infection. However, there has been no study on employing single photon emission computed tomography (SPECT) with using Tc-99m ciprofloxacin to image active pulmonary tuberculosis. Therefore, we conducted this study to assess the efficacy of Tc-99m ciprofloxacin SPECT for imaging active pulmonary tuberculosis. Twenty-one participants were enrolled in this prospective study. They were divided into two groups according to the clinical and radiological assessment. Group one (Gr. 1) consisted of five normal volunteers and six patients with inactive pulmonary tuberculosis. Group two (Gr. 2) consisted of ten patients with active pulmonary tuberculosis. SPECT was performed 3 h after injecting 555 MBq (15 mCi) of Tc-99m ciprofloxacin. The findings of Tc-99m ciprofloxacin SPECT were interpreted by a nuclear medicine specialist and then the results were analyzed according to the patients' clinical and radiological classifications. The results of Tc-99m ciprofloxacin SPECT were as follows: eight true-positive cases, ten true-negative cases, one false-positive case and two false-negative cases. The sensitivity and specificity was 80.0% and 90.0%, respectively. The positive predictive value was 88.9% and the negative predictive value was 83.3%. Conclusions Tc-99m ciprofloxacin SPECT is feasible for imaging active pulmonary tuberculosis. It is a useful nuclear-imaging method for discriminating between the active and inactive tuberculosis states in patients with a past medical history of pulmonary tuberculosis.

  3. New preparation method for /sup 99m/Tc-phytate

    International Nuclear Information System (INIS)

    A new method is described in which Pt-Sn or Sn-Sn electrodes are used to activate /sup 99m/TcO4-. The /sup 99m/Tc is incorporated into phytic acid by stannous ion released from a tin anode by the corrosive reaction. The most suitable pH for labeling phytic acid by this method was below 5 and the /sup 99m/Tc-phytate formed could be precipitated with Ca+2 at a pH above 3.5. Though /sup 99m/Tc-phytate is soluble in aqueous solution, it forms an insoluble species with Ca+2 in vivo and is trapped in the reticuloendothelial system. More than 93 percent of the /sup 99m/Tc-phytate localized in the liver of mice; here its biologic half-time is about 112 hr. The /sup 99m/Tc-phytate prepared by this method promises to be useful as a liver-scanning agent. Since our method does not require an applied electric potential, it appears to be one of the most convenient methods for labeling compounds with /sup 99m/Tc

  4. {sup 99m}Tc-sestamibi. Clinical applications

    Energy Technology Data Exchange (ETDEWEB)

    Bucerius, Jan [Maastricht Univ. Medical Center (Netherlands). Dept. of Nuclear Medicine and Cardiovascular Research Institute Maastricht; Ahmadzadehfar, Hojjat; Biersack, Hans-Juergen (eds.) [Universitaetsklinikum Bonn (Germany). Klinik und Poliklinik fuer Nuklearmedizin

    2012-07-01

    {sup 99m}Tc-sestamibi is a single-photon emission computed tomography (SPECT) radiotracer that was introduced into clinical routine for myocardial perfusion imaging more than two decades ago. Although today the main application of {sup 99m}Tc-sestamibi SPECT remains the imaging of myocardial perfusion, it is also an accepted and well-proven imaging technique for a variety of oncologic and non-oncologic applications, including brain, breast, and thyroid cancer and thyroid and parathyroid adenoma. Its efficacy in a range of indications ensures that {sup 99m}Tc-sestamibi SPECT will remain widely used despite the rapid diffusion of 18F-FDG PET. {sup 99m}Tc-Sestamibi - Clinical Applications provides a detailed and informative overview of almost all the oncologic and non-oncologic applications of {sup 99m}Tc-sestamibi SPECT, including several relatively rare indications. Different disease-related protocols for {sup 99m}Tc-sestamibi SPECT are presented, and for each disease a comprehensive summary of the relevant pathology and epidemiology is provided. Throughout, there is a strong emphasis on the practical aspects of use of this popular tracer, including instructions for the preparation of several commercially available tracer kits. Clinical practitioners will find this book to be an invaluable guide to the application and benefits of {sup 99m}Tc-sestamibi SPECT in both the inpatient and the outpatient setting. (orig.)

  5. Evaluation of {sup 99m}Tc-glucarate as a breast cancer imaging agent in a xenograft animal model

    Energy Technology Data Exchange (ETDEWEB)

    Gambini, Juan Pablo [Nuclear Medicine Center, Clinical Hospital, University of Uruguay, Montevideo, 11600 (Uruguay); Cabral, Pablo [Nuclear Investigations Center, School of Science, University of Uruguay, Montevideo, 11400 (Uruguay); Alonso, Omar [Nuclear Medicine Center, Clinical Hospital, University of Uruguay, Montevideo, 11600 (Uruguay); Savio, Eduardo [Department of Radiochemistry, School of Chemistry, University of Uruguay, Montevideo, 11800 (Uruguay); Daibes Figueroa, Said [Research Service, Harry S. Truman Veterans Memorial Hospital, Columbia, MO 65201 (United States); Zhang Xiuli [Research Service, Harry S. Truman Veterans Memorial Hospital, Columbia, MO 65201 (United States); Department of Biochemistry, University of Missouri, Columbia, MO 65211 (United States); Ma Lixin [Research Service, Harry S. Truman Veterans Memorial Hospital, Columbia, MO 65201 (United States); Department of Radiology, University of Missouri, Columbia, MO 65212 (United States); Deutscher, Susan L. [Research Service, Harry S. Truman Veterans Memorial Hospital, Columbia, MO 65201 (United States); Department of Biochemistry, University of Missouri, Columbia, MO 65211 (United States); Quinn, Thomas P., E-mail: quinnt@missouri.ed [Research Service, Harry S. Truman Veterans Memorial Hospital, Columbia, MO 65201 (United States); Department of Biochemistry, University of Missouri, Columbia, MO 65211 (United States); Department of Radiology, University of Missouri, Columbia, MO 65212 (United States)

    2011-02-15

    Introduction: The use of [{sup 99m}Tc]glucarate has been reported as an infarct-avid agent with the potential for very early detection of myocardial infarction. [{sup 99m}Tc]Glucarate has also been postulated as an agent for non-invasive detection of tumors. The aim of our study was to develop a Glucarate kit and evaluate [{sup 99m}Tc]glucarate as a potential cancer imaging agent in female SCID mice bearing human MDA-MB-435 breast tumors. Methods: Glucarate in a kit formulation was labeled with {sup 99m}Tc and evaluated for radiolabelling efficiency and radiochemical purity. The Glucarate kit stability was assessed by monthly quality controls. The pharmacokinetics of [{sup 99m}Tc]glucarate were determined in female SCID mice bearing MDA-MB-435 human breast carcinoma tumors at 0.5, 1, 2, 4 and 24 h. Nuclear imaging studies were performed with a micro-single photon emission tomography (SPECT)/computed tomography (CT) system at 2 h post injection, while magnetic resonance imaging (MRI) was employed for tumor morphology analysis and metastatic deposit localization. Results: The Glucarate kits exhibited a stable shelf life of 6 months. [{sup 99m}Tc]Glucarate was obtained with radiochemical purity greater than 95%. Biodistribution studies demonstrated moderate tumor uptake coupled with high renal clearance. Tumor-to-muscle ratios were 4.85 and 5.14 at 1 and 4 h post injection. MRI analysis showed tumors with dense cellular growth and moderate central necrosis. [{sup 99m}Tc]Glucarate uptake in the primary MDA-MB-435 shoulder tumors and metastatic lesions were clearly visualized with micro-SPECT/CT imaging. Conclusions: Selective tumor uptake and rapid clearance from nontarget organs makes [{sup 99m}Tc]glucarate a potential agent for breast cancer imaging that awaits validation in a clinical trial.

  6. Clinical studies of alveolar-capillary permeability using technetium-99m DTPA aerosol

    International Nuclear Information System (INIS)

    Soluble radioaerosols such as technetium-99m diethylene triamine pentacetate (DTPA) permit simple quantitative studies of alveolar-capillary permeability to be performed, since the submicronic aerosols are deposited mainly at the lung periphery and are cleared across the alveolar-capillary membrane. Regional alterations in permeability can also be noted using this radionuclide technique. We have measured the pulmonary epithelial permeability in normal subjects and the alteration in smokers, glue-sniffers, patients with inhalation burns, chronic obstructive pulmonary disease (COPD) and patients with lung metastases from thyroid cancer treated with radioiodine 131I. In the normal volunteers, the time taken for 50% of inhaled 99mTc DTPA to be cleared from the lungs (T1/2) was 66 minutes±1 sd of 12 mins. The smokers had a mean T1/2 of 20 mins±1 sd 4 min. In the hard-core glue-sniffing group, the majority were smokers who had stopped smoking and glue-sniffing for periods varying from 1 day to 42 days, and it was possible to note the changes in clearance times against period of abstinence. In the patients with inhalation burns, there was change in lung clearance arising from pulmonary epithelial damage; these patients showed increased rate of clearance (short T1/2) with mean T1/2 of 36 min±1 sd of 11 mins, while the retention images revealed regional lung damage in moderately severe inhalation burns. Twenty-four patients with COPD had inhalation scans done with Tc-99m tin colloid radioaerosol, and these images were compared with the perfusion lung scans done with 99mTc macroaggregated albumin (MAA); in general the perfusion images matched the defects noted in the inhalation scans. The 99mTc DTPA clearance rate in these patients was normal i.e. T1/2=78±14 mins. In the thyroid cancer patients with lung metastases, who had high doses of radioiodine treatment, the T1/2 values were normal or prolonged slightly, mean T1/2=76 min±23. (author)

  7. Alveolar epithelial permeability in patients with primary spontaneous pneumothorax as determined by Tc-99m DTPA aerosol scintigraphy

    International Nuclear Information System (INIS)

    Primary spontaneous pneumothorax (PSP) occurs subsequent to a disruption in the continuity of visceral pleura and escape of air into the pleural space. The cause of PSP is most often the rupture of subpleural blebs or bullae. It is usually difficult to detect evidence of pulmonary pathology. The purposes of the present study were to investigate the changes of pulmonary alveolar epithelial permeability in patients with PSP as determined by Tc-99m DTPA aerosol lung scintigraphy, to assess whether or not some differences exist between apical and basal parts of the lungs, and to determine the relationship between the clearance rate of Tc-99m DTPA and the pulmonary function test (PFT) results, the recurrence rate of PSP, and the percentage of pneumothorax in affected lung. Thirteen PSP patients (two females, 11 males; mean age 32.5±11.8 years) with normal chest X-ray were studied. Thirteen healthy non-smoking volunteers (1 female, 12 males; mean age, 35.8±10 years) were selected as a control group. Tc-99m DTPA aerosol lung scintigraphy and PFT were performed in all patients and controls. Clearance rates (%/min) of Tc-99m DTPA aerosol in right and left lung field, and apical and basal parts of each lung were calculated from dynamic images for 15 min. There was no significant difference (p>0.05) between patients and controls, or between apical and basal parts of each lung. No correlation was found between the clearance rate of Tc-99m DTPA and PFT results, the recurrence rate of PSP, or the percentage of pneumothorax. This study demonstrates that pulmonary epithelial permeability is not altered in PSP patients; the clearance rate of Tc-99m DTPA shows no difference between apical and basal parts of each lung. (author)

  8. Study of 99mTc-ECD instant kit

    International Nuclear Information System (INIS)

    The authors report the preparation of 99mTc-ECD instant kit, and a comparison of the instant kit with the ligand exchange kit. The radiochemical purity, stability, animal experiment and preliminary clinical trial showed that the instant kit possessed the following advantages: (1) The labelling yield (greater than 95%) and stability of 99mTc-ECD (the liberation of 99mTc 5 hr after preparation was less than 1%) were high; (2) The labelling method was simple, convenient and effective; (3) The quality of brain SPECT images was good. This instant kit is more suitable for the clinic application than the 2-step kit

  9. Evaluation of technetium 99m cyclobutylpropylene amine oxime as a potential brain perfusion imaging agent for SPET

    International Nuclear Information System (INIS)

    99mTc-labelled d,l-cyclobutylpropylene amine oxime (99mTc-CBPAO) has been developed as a brain imaging agent for single photon emission tomography (SPET). 99mTc-CBPAO can be prepared using a simple labelling procedure suitable for routine clinical use. It has a high in vitro stability, as has been demonstrated by high-pressure liquid chromatography (HPCL) analysis. This shows that 3 h after labelling, less than 5% of the primary lipophilic complex which is capable of crossing the blood-brain barrier (BBB) converts to a secondary hydrophilic complex. Brain uptake (% dose/g wet tissue) of 99mTc-CBPAO, determined at 5 and 30 min after injection in two groups of six adult male Sprague-Dawley rats, was found to be 0.74±0.06 and 0.73±0.13 (mean±SD), respectively. These values are not significantly different from those obtained repeating the experiment with 99mTc-labelled hexamethylpropylene amine oxime (99mTc-HMPAO) (0.72±0.15 at 5 min and 0.88±0.24 at 30 min after injection). Since the rat brain uptake of 99mTc-CBPAO remained unchanged for a period of time suitable for tomographic study, the comparison of the two tracers was extended to two groups of ten patients. The latter were affected by neurological and psychiatric disorders and were studied with SPET. Human brain uptake (% dose/cc cortical grey matter) of 99mTc-CBPAO and 99mTc-HMPAO were 3.04±0.57 and 4.22±0.46 (mean x 10-3±SD x 10-3), respectively, with a 32% significant difference. In two other groups of five patients, the first transit time-activity curves of the two tracers were compared. From the analysis of these curves we suggest that 99mTc-CBPAO has a higher binding effect on blood components and/or a higher degradation rate in blood than that of 99mTc-HMPAO. This may account for the reduced human brain uptake. In conclusion, SPET images of 99mTc-CBPAO reflect blood perfusion, and they have a good diagnostic quality. The main advantage of 99mTc-CBPAO is its in vitro stability; however, 99m

  10. {sup 99m}Tc-tetrofosmin or {sup 99m}Tc-sestamibi for double-phase parathyroid scintigraphy?

    Energy Technology Data Exchange (ETDEWEB)

    Froeberg, Alida C.; Valkema, Roelf [Department of Nuclear Medicine, Erasmus MC, Dr. Molewaterplein 40, 3015 GD Rotterdam (Netherlands); Bonjer, Jaap H. [Department of Surgery, Erasmus MC, Rotterdam (Netherlands); Krenning, Eric P. [Department of Nuclear Medicine, Erasmus MC, Dr. Molewaterplein 40, 3015 GD Rotterdam (Netherlands); Department of Internal Medicine, Erasmus MC, Rotterdam (Netherlands)

    2003-02-01

    Several years ago technetium-99m tetrofosmin was reported to localise parathyroid adenomas. The aim of this study was to compare the sensitivity of this radiopharmaceutical with that of {sup 99m}Tc-sestamibi using a double-phase parathyroid scintigraphy protocol. Scans of 12 patients were evaluated visually and lesion to thyroid ratios were calculated. Nine of the patients were subsequently operated on; a total of eight parathyroid adenomas or hyperplastic glands were histologically confirmed in seven of the patients, while in one patient a parathyroid carcinoma was histologically proven. All of these patients had positive {sup 99m}Tc-sestamibi scintigrams, whereas only two {sup 99m}Tc-tetrofosmin scintigrams were positive. With {sup 99m}Tc-sestamibi there was a significant increase in the lesion to thyroid ratio from 10 min to 90 min and 150 min p.i. which was not seen on scintigraphy with {sup 99m}Tc-tetrofosmin. This makes {sup 99m}Tc-tetrofosmin less suitable for double-phase parathyroid scintigraphy. (orig.)

  11. Labeling of scorpion venom with 99mTc and its biodistribution

    International Nuclear Information System (INIS)

    Labeling of scorpion venom (SV) was successfully achieved with 99mTc using direct chelating method. Venom was labeled with 99mTc using stannous chloride as reducing agent. Preliminary studies were done to establish the optimum conditions for obtaining the highest yield of the labeled venom. The labeling technique is effective, as a maximum labeling yield (97 %) was obtained after 30-min reaction time by using 80 μg SV in phosphate buffer of pH 7 and 25 μg Sncl2·2H2O at room temperature. Venom was injected into normal mice to determine the excretion pathway. Biodistribution studies in normal mice with SV shows rapid clearance of the venom from blood and tissue except for kidneys. The improvement of the immunotherapeutic treatment of envenomation requires a better knowledge of the biological actions of the SV since tissue distribution studies are very important for clinical purpose. (author)

  12. [Diagnosis of achalasia using 99m-Tc pertechnetate scintigraphy].

    Science.gov (United States)

    Mikosch, P; Gallowitsch, H J; Kresnik, E; Lind, P

    1997-06-01

    A 73-year-old patient presented a 99mTc scintiscan with a focal tracer accumulation left and caudal of the thyroid gland. Further investigations with sonography, CT, esophagoscopy and barium swallow provided the diagnosis of achalasia as the reason for focal 99mTc retention caudal of the thyroid gland. Explanation for 99mTc accumulation within the esophagus may be the nonspecific accumulation of 99mTc not only in the thyroid gland but also in the salivary glands. Excretion of the tracer follows with the saliva. Structural and motor disorders of the esophagus can thus lead to focal tracer retention within the esophagus simulating thyroid tissue. PMID:9395425

  13. Preparation of 99mTc-HYNIC-Annexin V

    Institute of Scientific and Technical Information of China (English)

    LAN Xiao-Li; ZHANG Yong-Xue

    2005-01-01

    99mTc-HYNIC-Annexin V was prepared for apoptosis radionuclide imaging in vivo. The expression plasmid of human Annexin V was constructed and expressed in E. coli. HYNIC was prepared and used as bifunctional chelating agent for 99mTc labeling. The radiochemical purity, radiolabeling yield and stability of 99mTc-HYNIC-Annexin V were studied. The coding sequence of human Annexin V gene was successfully cloned into the expression vector, and highly expressed in E. coli. A protein with a molecular weight of about 36kD could be induced and confirmed by Western Blotting and SDS-PAGE. Annexin V could be labeled with 99mTc using HYNIC, and showed high radiochemical purity of more than 90%. The radiolabeling yield could reach more than 90%. The reaction condition was moderate. It suggested that 99nTc-HYNIC-Annexin V may be a promising agent for apoptosis imaging and clinical application.

  14. Discordant results in Tc-99m tetrofosmin and Tc-99m sestamibi parathyroid scintigraphies; Resultados discordantes em cintilografias das paratireoides realizadas com tetrofosmin-99mTc e com sestamibi-99mTc

    Energy Technology Data Exchange (ETDEWEB)

    Duarte, Paulo Schiavom; Domingues, Fernanda C.; Santi Costa, Michele; Brandao, Cynthia; Oliveira, Marco A.C. de; Vieira, Jose G.H. [Fleury - Centro de Medicina Diagnostica, Sao Paulo, SP (Brazil)]. E-mail: paulo.duarte@fleury.com.br

    2007-10-15

    Parathyroid scintigraphies have been used to detect pathological parathyroid glands either before as well as after the parathyroid resection surgery in patients with hyperparathyroidism. One of the most utilized techniques to perform the studies is the double-phase images with Tc-99m sestamibi, which has been shown to be very accurate in the localization of enlarged parathyroid glands. Similar to Tc-99m sestamibi, Tc-99m tetrofosmin is a radiopharmaceutical initially developed to perform myocardial perfusion study that has been used to perform parathyroid scintigraphies. Although most of the papers suggest that the overall sensitivities of both radiopharmaceuticals are similar, there are some papers questioning the accuracy of Tc-99m tetrofosmin to detect abnormal parathyroid glands. In the present article, we report a case with discordant results by both methods. (author)

  15. 99mTc-sestamibi thyroid uptake in euthyroid individuals and in patients with autoimmune thyroid disease

    International Nuclear Information System (INIS)

    We investigated the biokinetics of 99mTc-sestamibi in the thyroid of euthyroid volunteers (EVs) and in patients with autoimmune thyroid diseases and determined the best time interval between 99mTc-sestamibi injection and calculation of uptake. Forty EVs, 30 patients with Graves' disease (GD), 15 patients with atrophic Hashimoto's thyroiditis (AHT) and 15 patients with hypertrophic Hashimoto's thyroiditis (HHT) underwent 99mTc-sestamibi thyroid scintigraphy. Dynamic images were acquired for 20 min, and static images were obtained 20 min, 60 min and 120 min post injection. Five-, 20-, 60- and 120-min uptake, time to maximal uptake (Tmax) and T1/2 of tracer clearance were calculated. Thyroid hormones and antibodies were measured. 99mTc-pertechnetate uptake was investigated in GD patients. Tmax was approximately 5 min in all four groups. The mean T1/2 value for EVs was similar to the GD value and lower than the HHT and AHT values. The mean (±SD) 5-min uptake was 0.13% (±0.05%) for EVs. The 5-min uptake in GD was higher than that in EVs(P99mTc-pertechnetate uptake (r=0.68). Uptake in HHT was higher than that in AHT (P=0.0003) and EVs (P=0.002). Uptake in AHT was lower than uptake in EVs (P=0.0001). Five minutes is the optimal time interval between 99mTc-sestamibi injection and calculation of thyroid uptake. Five-minute uptake differentiates euthyroid individuals from GD patients. There is a high correlation between 99mTc-sestamibi and 99mTc-pertechnetate uptake in GD. The reduced 99mTc-sestamibi uptake in AHT patients is probably due to glandular destruction and fibrosis. Inflammatory infiltrate and high mitochondrial density in thyrocytes possibly explain the increased uptake in GD and HHT. (orig.)

  16. 99mTc-MAMA-chrysamine G, a probe for beta-amyloid protein of Alzheimer's disease

    International Nuclear Information System (INIS)

    Chrysamine G (CG), an analogue of Congo red, is known to bind in vitro to the β-amyloid protein (Aβ 10-43) and to homogenates of several regions of the brain of Alzheimer's disease (AD) patients. We synthesised a conjugate of 2-(acetamido)-CG with a bis-S-trityl protected monoamide-monoaminedithiol (MAMA-Tr2) tetraligand, which was efficiently deprotected and labelled with a 75% yield with technetium-99m, to obtain 99mTc-MAMA-CG. In mice, 99mTc-MAMA-CG was cleared mainly by the hepatobiliary system, resulting in a fast blood clearance. Brain uptake of 99mTc-MAMA-CG was low. Co-injection with the blood pool tracer iodine-125 human serum albumin (125I-HSA) demonstrated a brain/blood activity ratio for 99mTc-MAMA-CG that was significantly higher than that for 125I-HSA (t test for dependent samples, P99mTc-MAMA-CG to cross the blood-brain barrier. In vitro autoradiography demonstrated pronounced binding of 99mTc-MAMA-CG to β-amyloid deposits in autopsy sections of the parietal and occipital cortex of an AD patient as compared with controls. Adding 10 μM Congo red during incubation displaced the binding of 99mTc-MAMA-CG. Congo red staining and autoradiography identified the same lesions. 99mTc-MAMA-CG seems to bind selectively to β-amyloid deposition in human brain parenchyma and blood vessels in vitro and thus might be a lead compound for further development of a useful tracer agent for the in vivo diagnosis of Alzheimer's disease. (orig.)

  17. Perfusion lymphoscintigraphy using sup 99m Tc-human serum albumin in patients with treated uterine cancer

    Energy Technology Data Exchange (ETDEWEB)

    Kataoka, Masaaki; Kawamura, Masashi; Nishiyama, Yasuyuki; Itoh, Hisao; Hamamoto, Ken; Hamada, Katsuyuki; Matsuura, Shumpei (Ehime Univ., Shigenobu (Japan). School of Medicine)

    1991-07-01

    Perfusion lymphoscintigraphy was performed by subcutaneous injection of 7.4 MBq (0.2 mCi) {sup 99m}Tc-human serum albumin ({sup 99m}Tc-HSA) in 25 patients with uterine cancer treated by operation and/or irradiation. Radioactivity at the injection site was counted for 3 min at 10 min (a) and at 3 hr (b) after injection, and the clearance of {sup 99m}Tc-HSA was defined as (1-(b)/(a))x100(%) ((a) and (b) were corrected for decay of the isotope). The clearance in legs with lymphedema was significantly more delayed than those in legs without lymphedema in patients treated with both operation and irradiation (16.6{+-}7.7% vs 34.9{+-}9.3%; p<0.01) and in patients treated with radiation therapy alone (33.1{+-}7.4 vs 48.0{+-}5.6; p<0.01). The clearances in edematous legs in a case which had developed venous occulusion after operation were 44.2% and 41.7%, which were almost the same as those in the non-edematous patients treated with operation alone. Clearance in patients treated with both operation and irradiation were significantly more delayed than those in patients treated with a single modality of operation or irradiation (30.1{+-}11.4% vs 41.9{+-}8.9%, 42.0{+-}9.7%, respectively; p<0.01). Radiation doses at points B were well correlated with clearance of {sup 99m}Tc-HSA (p<0.05). These data suggest that perfusion lymphoscintigraphy using {sup 99m}Tc-HSA is useful for evaluating the patients with lymphedema and for differentiating it from the edema caused by the other mechanisms. It is also suggested that radiation dose is one of factors in the occurrence of lymphedema. (author).

  18. Synthesis, radiolabeling and in vivo biological evaluation of {sup 99m}Tc-labeled MAG{sub 3}-based bisnitroimidazole complexes as tumor hypoxia markers

    Energy Technology Data Exchange (ETDEWEB)

    Mei, Lei; Chu, Taiwei [Peking Univ., Beijing (China). Beijing National Laboratory for Molecular Sciences, Radiochemistry and Radiation Chemistry

    2014-04-01

    Hypoxia, as a common phenomenon in solid tumors, is of interest for its relationship with resistance to tumor therapies and malignant progression of tumor. The noninvasive nuclear medical imaging technique using hypoxia markers is an important method for the detection of tumor hypoxia. The aim of current study is designing tumor hypoxia markers with hypoxia selectivity and improved properties. Two MAG{sub 3}-based bisnitroimidazole compounds were synthesized and purified by semi-preparative HPLC. Both the MAG{sub 3} derivatives were labeled with {sup 99m}Tc-oxo-technetium core via stannous tartrate exchange method in high yields (> 95%). The {sup 99m}Tc-MAG{sub 3} complexes were stable at 37 C, 4 h after preparation, and were more hydrophilic than {sup 99m}Tc-MAMA complexes. As biodistribution results showed, clearances of background activity for both the complexes were fast and they were excreted mainly through the hepatobiliary tract and part of renal tract. Although tumor uptakes of {sup 99m}Tc-MAG{sub 3}-B2NIL were lower than those of {sup 99m}Tc-MAG{sub 3}-B4NIL, tumor-to-blood ratios of {sup 99m}Tc-MAG{sub 3}-B2NIL showed an increasing trend and were better than those of {sup 99m}Tc-MAG{sub 3}-B4NIL after 2 h due to their different blood clearances. Tumor-to-muscle ratios of {sup 99m}Tc-MAG{sub 3}-B2NIL and {sup 99m}Tc-MAG{sub 3}-B4NIL were similar. Comparing with {sup 99m}Tc-MAMA complexes, {sup 99m}Tc-MAG{sub 3}-B2NIL with better tumor-to-blood ratios exhibits improved feature for hypoxia imaging, though it has lower tumor uptake than {sup 99m}Tc-MAMA complexes. (orig.)

  19. Biodistribution and biological characteristics of p-[(bis-carboxymethyl) aminomethyl carboxyamino] hippuric acid (Pahida) labelled with technetium-99m. Establishment of pharmacokinetics parameters through compartmental model

    International Nuclear Information System (INIS)

    Biologic distribution of p- [(bis-carboxymethylaminomethyl carboxyamino)] hippuric acid (PAHIDA) labeled with sup(99m)Tc in Wistar rats, showed a selective renal uptake among the other organs and tissues. The compound is predominantly eliminated by urinary tract, with small enterohepatic percent of excretion Chromatographic analysis of urine showed the product and possible metabolites. PAHIDA- sup(99m)Tc blood clearance is relatively rapid and a good percent is transported by plasmatic proteins. The percent binding to the erythrocytes is significant after one hour, this is due probably to hydrolysed technetium. The extrapolation of the plasmatic curve denoted the existence of three exponentials, suggesting a model with three compartments: central or intravascular and two peripherics or extravasculars - rapid and slow exchange (retention). Exponential's half life and the transfer constant (k) among the compartments were determined. The compound retention was reaffirmed by whole body determination. The decomposition of the curve in two exponentials allowed to assess the component's half-life. The compartmental model proposed in agreement with the experimental results, showed the complex retention that may be related the binding with the blood components, the possibility of renal metabolization or a structural impediment in the interaction with the tubular cells receptors. (author)

  20. Fab(nimotuzumab)-HYNIC-99mTc: Antibody Fragmentation for Molecular Imaging Agents.

    Science.gov (United States)

    Calzada, Victoria; García, María Fernanda; Alonso-Martínez, Luis Michel; Camachoc, Ximena; Goicochea, Enzo; Fernández, Marcelo; Castillo, Abmel Xiques; Díaz-Miqueli, Arlhee; Iznaga-Escobar, Normando; Montaña, René Leyva; Alonso, Omar; Gambini, Juan Pablo; Cabral, Pablo

    2016-01-01

    Finally, fast blood clearance nimotuzumab is a humanized monoclonal antibody that recognise, with high specific affinity, the epidermal growth factor receptor (EGF-R) which play an important role in the growth process associated with many solid tumors. In this work, the whole antibody was digested with papain in order to generate a Fab fragment, derivatized with NHS-HYNIC-Tfa and radiolabel with technetium-99m (99mTc) as a potential agent of molecular imaging of cancer. Both, whole and fragment radiolabels were in-vivo and in-vitro characterized. Radiolabeling conditions with Tricine as coligand and quality controls were assessed to confirm the integrity of the labeled fragment. Biodistribution and imaging studies in normal and spontaneous adenocarcinoma mice were performed at different times to determine the in-vivo characteristics of the radiolabel fragment. Tumor localization was visualized by conventional gamma camera imaging studies, and the results were compared with the whole antibody. Also, an immunoreactivity assay was carried out for both. The results showed clearly the integrity of the nimotuzumab fragment and the affinity by the receptor was verified. Fab(nimotuzumab)-HYNIC was obtained with high purity and a simple strategy of radiolabeling was performed. Finally, a fast blood clearance was observed in the biodistribution studies increasing the tumor uptake of Fab(nimotuzumab)- HYNIC-99mTc over time, with tumor/muscle ratios of 3.81 ± 0.50, 5.16 ± 1.97 and 6.32 ± 1.98 at 1 h, 4 h and 24 h post injection. Urinary excretion resulted in 32.89 ± 3.91 %ID eliminated at 24 h. Scintigraphy images showed uptake in the tumor and the activity in non-target organs was consistent with the biodistribution data at the same time points. Hence, these preliminary results showed important further characteristic of Fab(nimotuzumab)-HYNIC-99mTc as a molecular imaging agent of cancer. PMID:26961312

  1. Fab(nimotuzumab)-HYNIC-99mTc: Antibody Fragmentation for Molecular Imaging Agents.

    Science.gov (United States)

    Calzada, Victoria; García, María Fernanda; Alonso-Martínez, Luis Michel; Camachoc, Ximena; Goicochea, Enzo; Fernández, Marcelo; Castillo, Abmel Xiques; Díaz-Miqueli, Arlhee; Iznaga-Escobar, Normando; Montaña, René Leyva; Alonso, Omar; Gambini, Juan Pablo; Cabral, Pablo

    2016-01-01

    Finally, fast blood clearance nimotuzumab is a humanized monoclonal antibody that recognise, with high specific affinity, the epidermal growth factor receptor (EGF-R) which play an important role in the growth process associated with many solid tumors. In this work, the whole antibody was digested with papain in order to generate a Fab fragment, derivatized with NHS-HYNIC-Tfa and radiolabel with technetium-99m (99mTc) as a potential agent of molecular imaging of cancer. Both, whole and fragment radiolabels were in-vivo and in-vitro characterized. Radiolabeling conditions with Tricine as coligand and quality controls were assessed to confirm the integrity of the labeled fragment. Biodistribution and imaging studies in normal and spontaneous adenocarcinoma mice were performed at different times to determine the in-vivo characteristics of the radiolabel fragment. Tumor localization was visualized by conventional gamma camera imaging studies, and the results were compared with the whole antibody. Also, an immunoreactivity assay was carried out for both. The results showed clearly the integrity of the nimotuzumab fragment and the affinity by the receptor was verified. Fab(nimotuzumab)-HYNIC was obtained with high purity and a simple strategy of radiolabeling was performed. Finally, a fast blood clearance was observed in the biodistribution studies increasing the tumor uptake of Fab(nimotuzumab)- HYNIC-99mTc over time, with tumor/muscle ratios of 3.81 ± 0.50, 5.16 ± 1.97 and 6.32 ± 1.98 at 1 h, 4 h and 24 h post injection. Urinary excretion resulted in 32.89 ± 3.91 %ID eliminated at 24 h. Scintigraphy images showed uptake in the tumor and the activity in non-target organs was consistent with the biodistribution data at the same time points. Hence, these preliminary results showed important further characteristic of Fab(nimotuzumab)-HYNIC-99mTc as a molecular imaging agent of cancer.

  2. Labelling of biological structures with technetium 99 m

    International Nuclear Information System (INIS)

    The labelling of red blood cells (RBC) with technetium 99m (99m Tc) depends on several factors, as the stannous ion (Sn++) concentration, the time and temperature of incubation, the anticoagulant utilized, the presence of plasma proteins (PP) and others. Although the blinding of 99m Tc with hemoglobin and PP are similar, they appear to have specific characteristics as demonstrated by precipitation with alcohol, acetone, trichloroacetic acid, hydrochloric acid and mercury chloride. The bacterial cultures labeled with Technetium-99m, at optimal Sn++ ion concentration, presents a large stability and their viability is not altered by this treatment. The electrophoretic mobility, the hydrophobicity, the cationized ferritin distribution and the adherence to human buccal epithelial cells are not modified either. The possibility of labelling with 99m Tc of planaria and cercariae of Schistossoma mansoni evaluative cycle increases the utilization of this radionuclide to an experimental level. The results described with the labelling of these biological structures with 99m Tc demonstrated that stable labeled and viable operations are obtained. (author)

  3. Tumour imaging using technetium-99m-citrate

    International Nuclear Information System (INIS)

    Sixteen patients with soft tissue malignancy or fibroadenoma of the breast (Group A) were imaged using 99mTc-citrate. Majority of the patients (n=14) has new untreated lesions. Appreciable skeletal uptake of the tracer was serendipitously noticed in all cases. One of these had widespread bone metastases seen almost identically in 99mTc-citrate and 99mTc-MDP studies. Accordingly, 10 patients (Group B) having more than 40 malignant lesions on the bone scan underwent 99mTc-citrate study. In group A, accumulation of the tracer was seen in all malignant breast nodules and axillary lymphnode mass (n=4), medullary carcinoma of the thyroid along with its metastasis and a carcinoid (n=4) and an ovarian malignancy. Uptake and outflow pattern could differentiate fibroadenoma (n=3) from carcinoma of the breast. No significant uptake was seen in liver secondaries (n>10), lymphoma lesions (n=5), papillary carcinoma of thyroid, renal cell and embryonal cell carcinoma. In group B patients, the radiotracer accumulated well in the metastatic lesions while there was distinctly lesser uptake in normal/degenerated joints compared to the bone scan. The study shows potential of the tracer in imaging soft tissue malignancies. Bone scanning with 99mTc-citrate is an interesting possibility since mechanism of its uptake appears to be different to 99mTc-MDP. (author)

  4. Technetium-99m pentavalent dimercaptosuccinic acid imaging for pituitary adenomas

    International Nuclear Information System (INIS)

    The authors performed scintigraphy using 99mTc(V)-DMSA (Technetium-99m pentavalent dimercaptosuccinic acid) on patients with pituitary adenomas. Three non-functioning (100%), 2 GH-secreting (67%), 4 PRL-secreting (80%), and zero ACTH-secreting (0%) adenomas concentrated the 99mTc(V)-DMSA, but all 5 of the non-adenomatous lesions and 1 normal pituitary gland did not. There was no significant relationship between tumor-to-background ratios and tumor sizes, or the serum hormone level. The 99mTc(V)-DMSA scintigraphy showed an overall sensitivity of 69% (9/13) in detecting pituitary adenomas, which increased to 82% for non-functioning, GH-secreting and PRL-secreting adenomas. In conclusions, 99mTc(V)-DMSA was found to be a suitable radiotracer for detecting pituitary adenomas. But further studies are necessary to define the processes that concentrate 99mTc(V)-DMSA and their role in pituitary adenomas. (author)

  5. Modulation of the multidrug resistance P-glycoprotein: Detection with technetium-99m-sestamibi in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Luker, G.D.; Fracasso, P.M.; Dobkin, J.; Piwnica-Worms, D. [Washington Univ. School of Medicine, St. Louis, MO (United States)

    1997-03-01

    Overexpression of the multidrug resistance (MDR1) P-glycoprotein (Pgp) has been documented in nearly all forms of human cancers and increased levels of Pgp in some tumors correlate with poor response to treatment. Technetium-99m-sestamibi has recently been validated as a Pgp transport substrate. Pgp is also normally expressed along the biliary canalicular surface of hepatocytes and the luminal side of proximal tubule cells in the kidney, while not expressed in heart. Focused on these organs with known Pgp status, we present the findings on {sup 99m}Tc-sestamibi showed normal, prompt clearance of the radiotracer from the liver and kidneys relative to the heart. After administration of the Pgp modulator, {sup 99m}Tc-sestamibi was selectively retained in the liver and kidneys. Hepatobiliary and renal clearance of {sup 99m}Tc-sestamibi are Pgp-mediated, and inhibition of Pgp transport in these organs can be successfully imaged using {sup 99m}Tc-sestamibi in patients. Similar results might be expected with this and related radiopharmaceuticals for functional imaging of Pgp transport and modulation in tumors. 34 refs., 2 figs.

  6. Poor Tc-99m dimercaptosuccinic acid uptake, re-evaluation with Tc-99m MAG3 scintigraphy in Lowe syndrome

    OpenAIRE

    Koca, Gokhan; Atilgan, Hasan Ikbal; Demirel, Koray; Diri, Akif; KORKMAZ, Meliha

    2011-01-01

    Tc-99m dimercaptosuccinic acid (DMSA) is filtered through the glomeruli and reabsorbed by the proximal tubules as low molecular weight proteins. In Lowe syndrome this mechanism is impaired and so poor DMSA uptake is seen. Poor DMSA uptake was shown in very few studies, but none mentioned normal Tc-99m MAG3 uptake. In this case, the patient had poor DMSA uptake, normal MAG3 uptake and a neurogenic bladder in anterior to the left kidney that attenuates left kidney.

  7. A correlative study between {sup 99m}Tc-ESTCPTA and {sup 99m}Tc-MIBI in rats

    Energy Technology Data Exchange (ETDEWEB)

    Unak, Perihan E-mail: unak@bornova.ege.edu.tr; Enginar, Huseyin; Zuemruet Biber, F.; Yurt Lambrecht, Fatma; Aslani, M.A.A.; Ozkilic, Hayal

    2002-11-01

    Tissue distribution of the {sup 99m}Tc labeled derivative of the estrogen compound 3,17-{alpha}-estradiolyl propyl 1,4,8,11-tetraazacyclotetradecanyl-1-(4-methylbenzoic acid) ester (ESTCPTA), which has an 3,17-{alpha}-estradiolyl propinol coupled to 1-(4-methylbenzoic acid)1,4,8,11-tetraazacyclotetradecane (CPTA), was compared to {sup 99m}Tc-MIBI (methoxyisobutyl isonitrile) in female Albino Wistar rats. Tissues of interest included lung, liver, heart, kidneys, spleen, stomach, intestines, pancreas, muscle, blood, breast, ovary, fat, and uterus. {sup 99m}Tc-ESTCPTA uptake by the uterus and ovary, as ER-rich tissues, was highly selective. Maximum uptakes for {sup 99m}Tc-MIBI and {sup 99m}Tc-ESTCPTA are 90 min in breast, ovary and uterus. The pancreas also showed significant receptor saturated and unsaturated ratios for {sup 99m}Tc-ESTCPTA. Results are sufficiently encouraging to generate further evaluation of these and related compounds as possible estrogen receptor based tumor imaging and therapeutic agents in estrogen-rich tissues.

  8. {sup 99m}Tc-ECD and {sup 99m}Tc-HM-PAO SPECT in five patients with MELAS

    Energy Technology Data Exchange (ETDEWEB)

    Katagiri, Shinako; Nishimaki, Hiroshi; Kitano, Masashi; Horiike, Shigeharu; Kan, Shinichi; Ishii, Katsumi; Matsubayashi, Takashi; Sakai, Fumihiko [Kitasato Univ., Sagamihara, Kanagawa (Japan). School of Medicine

    1998-03-01

    Cerebral perfusion was studied in five patients with mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS), using single photon emission computed tomography (SPECT) with {sup 99m}Tc-ethyl cysteinate dimer ({sup 99m}Tc-ECD) or {sup 99m}Tc-hexamethyl propyleneamine oxime ({sup 99m}Tc-HM-PAO). In four cases, regional cerebral blood flow (rCBF) was evaluated by the method reported by Mastuda et al. Immediately after the stroke-like episodes, accumulation of the tracer was relatively increased in the temporooccipital lobe, and also increased rCBF was shown in the same area. However, the region showed decreased radioactivity at the chronic stage, and rCBF decreased also. These findings are consistent with positron emission tomography (PET) at the acute stage and autopsy. {sup 99m}Tc-ECD SPECT and {sup 99m}Tc-HM-PAO SPECT may be useful in the diagnosis and assessment of the progress of the MELAS. (author)

  9. Tc-99m and Re-186 complexes of tetraphosphonate ligands and their biodistribution pattern in animal models

    International Nuclear Information System (INIS)

    The syntheses of four α-aminomethyl phosphonates and their complexation studies with 99mTc and 186/188Re are reported. Complexation conditions were standardized to give maximum yields, which ranged from 90-97%. The yields of complexation were estimated by paper chromatography. The 99mTc complexes were stable for more than 4 h, while the 186/188Re complexes were stable for 3-8 days when stored at 4 deg. C. Biodistribution of these complexes in Wistar rats were carried out, and the uptake in bone and other soft tissue are detailed. Bone uptake of the 99mTc complexes varied from 40-60% at 30 min postinjection depending on the ligands. The uptake in soft tissue was minimum with all the complexes. A comparison of the biodistribution studies of the 99mTc complexes with that of the well-established radiopharmaceutical 99mTc-MDP was carried out for the purpose of evaluating the efficacy of the radiopharmaceutical preparation with the complexes of these ligands. The bone uptake of the 186/188Re complexes varied from 19-28% corresponding to 1.6-3% per g at 3 h postinjection. The residual activity in both 99mTc and 186/188Re complexes showed renal clearance

  10. In vivo Behaviour of Technetium-99m Labelled N, N' -bis-(1-Carboxy-2-Mercaptoethyl)-Ethylenediamine(EC)

    International Nuclear Information System (INIS)

    N,N-bis-(1-carboxy-2-mercaptoethyl)ethylenediamine(EC), a precursor for potential new generation technetium radiopharmaceutical for renal function studies, was synthesized in simple two step method. High percentage labelling (90%) was achieved during its radiopharmaceutical preparation with Tc-99m pertechnetate in presence of stannous chloride. Its renal clearance in experimental animals was found to be better than that of 131 I-ortho-iodohippuric acid and comparable to that of the recently developed 99m Tc-MAG3. 13 refs., 7 tables (author)

  11. Technetium-99m nitrido radiopharmaceuticals with unprecedented biological properties

    Directory of Open Access Journals (Sweden)

    Adriano Duatti

    2002-09-01

    Full Text Available The chemical methods for the production of technetium-99m radiopharmaceuticals containing a terminal TcºN triple bond have been established more than a decade ago. From that time, the chemistry of nitrido Tc-99m complexes has provided a highly efficient tool for the design and preparation of novel classes of diagnostic agents, and a number of potentially useful radiopharmaceuticals have been discovered. In particular, nitrido technetium-99m tracers have been developed for heart perfusion imaging. In this short review, the chemical and biological properties of the neutral myocardial perfusion tracer bis(N-ethoxy, N-ethyl-dithiocarbamato nitrido Tc-99m (TcN-NOEt will be summarized along with the preparation and preliminary biological evaluation of the first class of monocationic nitrido technetium-99m radiopharmaceuticals exhibiting improved biodistribution properties closer to those expected for an ideal perfusion imaging agent.Os métodos químicos para produção de radiofármacos marcados com tecnécio-99m contendo a ligação tripla terminal TcºN foram estabelecidos há mais de uma década. Desde esta época, a química dos complexos nitridos marcados com 99mTc tem sido uma ferramenta altamente eficiente para o desenho e preparo de novas classes de agentes para diagnóstico e, foi descoberto um número de radiofarmacos potencialmente úteis. Nesta pequena revisão, as propriedades biológicas e químicas do traçador para perfusão miocárdica neutra, o bis(N-etoxi, N-etil-ditiocarbamato nitrido 99mTc (TcN-NOEt, serão resumidas junto com o preparo e avaliação biológica preliminar da primeira classe de radiofármacos nitrido monocatiônico marcado com tecnécio-99m que exibe melhores propriedades em relação à biodistribuição, mais próximas daquelas esperadas para um agente perfusor ideal para imagens.

  12. Labeling cellular elements of blood with Technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Dewanjee, M.K.

    1990-08-01

    The purpose of this proposal is to develop new technique of labeling platelets and white cells with Tc-99m radionuclide. The conditions of labeling canine platelets and white cells with the lipid-soluble Tc-99m HMPAO have been optimized. The function of labeled platelets were evaluated by the determination of platelet survival time and recovery and these values were compared with that of In-111 tropolone labeled platelets. We developed the bilateral femoral catheterization model for the evaluation of platelet-thrombosis on control and heparin-bonded catheters in dogs. We are evaluating platelet thrombosis in the hollow-fiber hemodialyzer with Tc-99m and In-111 labeled platelets. We have developed the flow-loop for in vitro studies and are using a pig model for quantitation of platelet-consumption during hemodialysis. We are currently evaluating the new technique of platelet and white cell-labeling with Tc-99m and testing them in animal models of thrombosis and infection (osteo-myelitis). We are also using the Tc-99m HMPAO labeled mixed white cells in the early diagnosis (3-hour post-injection) of acute and chronic infection in patients and comparing the results with that of IN-111 oxine labeled white cells.

  13. Technique using Technetium-99m-MIBI Uptake Kinetics

    Directory of Open Access Journals (Sweden)

    Shinro Matsuo

    2013-04-01

    Full Text Available Mitochondria play an important role in energy production for the cell. The proper function of a myocardial cell largely depends on the functional capacity of the mitochondria.  Therefore it is necessary to establish a novel and reliable method for a non-invasive assessment of mitochondrial function and metabolism in humans. Although originally designed for evaluating myocardial perfusion, 99mTc-MIBI can be also used to evaluate cardiac mitochondrial function. In a clinical study on ischemic heart disease, reverse redistribution of 99mTc-MIBI was evident after direct percutaneous transluminal coronary angioplasty. The presence of increased washout of 99mTc-MIBI was associated with the infarct-related artery and preserved left ventricular function. In non-ischemic cardiomyopathy, an increased washout rate of 99mTc-MIBI, which correlated inversely with left ventricular ejection fraction, was observed in patients with congestive heart failure. Increased 99mTc-MIBI washout was also observed in mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS and in doxorubicin-induced cardiomyopathy. Noninvasive assessment of cardiac mitochondrial function could be greatly beneficial in monitoring possible cardiotoxic drug use and in the evaluation of cardiac damage in clinical medicine.

  14. Synthesis and formulation of 99m Tc-ECD radiopharmaceutical

    International Nuclear Information System (INIS)

    Nuclear medicine is a medical specialty which uses radioactive compounds (radionuclides) for diagnostic and therapeutic purposes. 99m Tc is the more common radionuclide used in many studies in nuclear medicine because its advantages: it has a photopeak of 140 KeV and a half-life of 6 hours; it can be eluted from a Molybdenum 99 generator, so radiopharmaceuticals can be prepared on site. Ethyl cysteine dimer (ECD) labelled with reduced Technetium 99m has been purposed recently as a promising radiopharmaceutical for brain perfusion imaging 99m Tc-ECD is a lipophilic neutral complex which cross the brain blood barrier and show high brain uptake. The objective of this work was synthesize and to design a freeze dried formulation for the instant preparation of 99m Tc-ECD complex useful for brain perfusion imaging. We obtained a freeze dried stable formulation for the preparation of 99m Tc-ECD kit with a radiochemical purity higher than 90 %, which fulfills with the quality control of radiopharmaceuticals. Furthermore, we developed analytic techniques for the determination of the different chemical compounds into the lyophilized kit. (Author)

  15. Retention of 99mTc-bicisate in the human brain after intracarotid injection

    DEFF Research Database (Denmark)

    Friberg, L; Andersen, A R; Lassen, N A;

    1994-01-01

    99mTc-bicisate (ECD) was injected as a bolus into the internal carotid artery, and cerebral uptake and retention were recorded with fast-rotating single photon emission computed tomography (SPECT) equipment in four patients suffering from temporal lobe epilepsy. Quantitative regional cerebral blood...... flow (rCBF) was measured tomographically with the 133Xe inhalation technique. We applied a three-compartment kinetic model and algorithms modified from a previous analysis of 99mTc d,l-hexamethylpropyleneamine oxide (HM-PAO) kinetics. The bicisate brain uptake and retention curve was very similar...... to incomplete retention of the deesterified hydrophilic metabolites. Computerized curve-fitting on data from three patients gave average kinetic values for the first-passage (unilateral) extraction of E = 0.60 (range, 0.59-0.61); the overall retained fraction of the tracer supplied was R = 0.44 (0...

  16. Development and validation of methodology for technetium-99m radiopharmaceuticals using high performance liquid chromatography (HPLC)

    International Nuclear Information System (INIS)

    Radiopharmaceuticals are compounds, with no pharmacological action, which have a radioisotope in their composition and are used in Nuclear Medicine for diagnosis and therapy of several diseases. In this work, the development and validation of an analytical method for 99mTc-HSA, 99mTc-EC, 99mTc-ECD and 99mTc-Sestamibi radiopharmaceuticals and for some raw materials were carried out by high performance liquid chromatography (HPLC). The analyses were performed in a Shimadzu HPLC equipment, LC-20AT Prominence model. Some impurities were identified by the addition of a reference standard substance. Validation of the method was carried out according to the criteria defined in RE n. 899/2003 of the National Sanitary Agency (ANVISA). The results for robustness of the method showed that it is necessary to control flow rate conditions, sample volume, pH of the mobile phase and temperature of the oven. The analytical curves were linear in the concentration ranges, with linear correlation coefficients (r2) above 0.9995. The results for precision, accuracy and recovery showed values in the range of 0.07-4.78%, 95.38-106.50% and 94.40-100.95%, respectively. The detection limits and quantification limits varied from 0.27 to 5.77 μg mL-1 and 0.90 to 19.23 μg mL-1, respectively. The values for HAS, EC, ECD and MIBI in the lyophilized reagents were 8.95; 0.485; 0.986 and 0.974 mg L-1, respectively. The mean radiochemical purity for 99mTc-HSA, 99mTc-EC, 99mTc-ECD and 99mTc-Sestamibi was (97.28 ± 0.09)%, (98.96 ± 0.03)%, (98.96 ± 0.03)% and (98.07 ± 0.01)%, respectively. All the parameters recommended by ANVISA were evaluated and the results are below the established limits. (author)

  17. Comparison of relative renal function measured with either 99mTc-DTPA or 99mTc-EC dynamic scintigraphies with that measured with 99mTc-DMSA static scintigraphy

    Directory of Open Access Journals (Sweden)

    F. C. Domingues

    2006-08-01

    Full Text Available OBJECTIVE: The aim of this study was to compare the renal function measured with either 99mTc-DTPA or 99mTc-EC dynamic scintigraphies with that measured using 99mTc-DMSA static scintigraphy. METHODS: the values of relative renal function measured in 111 renal dynamic scintigraphies performed either with 99mTc-DTPA (55 studies or with 99mTc-EC (56 studies were compared with the relative function measured using 99mTc-DMSA static scintigraphy performed within a 1-month period. The comparisons were performed using Wilcoxon signed rank test. The number of 99mTc-DTPA and 99mTc-EC studies that presented relative renal function different by more than 5% from that measured with 99mTc-DMSA, using chi square test were also compared. RESULTS: the relative renal function measured with 99mTc-EC is not statistically different from that measured with 99mTc-DMSA (p = 0.97. The relative renal function measured with 99mTc-DTPA was statistically different from that measured using 99mTc-DMSA, but with a borderline statistical significance (p = 0.05. The number of studies with relative renal function different by more than 5% from that measured with 99mTc-DMSA is higher for the 99mTc-DTPA scintigraphy (p = 0.04 than for 99mTc-EC. CONCLUSION: the relative renal function measured with 99mTc-EC dynamic scintigraphy is comparable with that measured with 99mTc-DMSA static scintigraphy, while the relative renal function measured with 99mTc-DTPA dynamic scintigraphy presents a significant statistical difference from that measured with 99mTc-DMSA static scintigraphy.

  18. 99mTc-Ciprofloxacin for diagnosis of bacterial infection

    International Nuclear Information System (INIS)

    Preparation of '9'9'mTc-Ciprofloxacin for diagnosis of bacterial infection was investigated in this research. Factors including pH, injected dose and the purity of 99mTc-Ciprofloxacin were determined. The optimal labeling condition of 99mTc-Ciprofloxacin was found to be at pH 4.0-5.0. In terms of efficiency, the injected dose per gram of infected area was 0.25-0.56, which lasted for the duration of 1-3 h after injection. The radiochemical purity remained >90% with stability for the duration of 6 h. Therefore, this study has demonstrated the preparation of sterile, pyrogen-free and non-toxic 99mTc-Ciprofloxacin.

  19. Tc-99m ciprofloxacin scintigraphy in evaluation of orthopedic infections

    International Nuclear Information System (INIS)

    Identification of bacterial infection in orthopedic practice is an important issue for appropriate management. Conventional radiography including CT examination has limited role in such a situation. A Tc-99m Ciprofloxacin has been developed to localize bacterial infection in order to differentiate infection from inflammation. Critical evaluation of role of Tc-99m Ciprofloxacin scintigraphy in orthopedic practice was done in the present study. 104 patients with suspected infection in orthopedic clinic were taken up for Tc-99m Ciprofloxacin scintigraphy. A dose of 370 MBq of Tc-99m Ciprofloxacin was injected intravenously. Imaging was performed using Siemens integrated DIACAM camera with low energy all parallel or high-resolution collimators. Multiple spot views at serial time (1 hour, 4 hours and 24 hours) were obtained in 256 x 256 matrix size in ICON computer. Focal area of abnormal radionuclide concentration, which increased with time, was considered to be the pattern suggestive of presence of infection. In cases of equivocal qualitative appearance, quantitation of uptake was ascertained by the count statistics obtained using region of interest program. Final diagnosis of sepsis was based on microbiological examination or evidence of beneficial response to antibiotic therapy. Tc-99 Ciprofloxacin scintigraphy showed most favourable results in peripheral tubercular as well as non-tubercular osteomyelitis, tubercular arthritis and in cases with prosthesis (including vascular stent). It showed limited role in Pott's spine. The best sensitivity, specificity and accuracy of Tc-99m Ciprofloxacin using the defined criteria was 90%, 84% and 88% respectively in cases with non-tubercular osteomyelitis. Serial imaging up to 24 hours was required in ∼ 70% of cases. In ∼ 30% of cases quantification was required to form an opinion. We conclude that Tc-99m Ciprofloxacin is a highly useful technique in the management of infection in orthopedic practice. The procedure should

  20. Transferrin receptor targeted imaging using the Tc-99m transferrin

    Energy Technology Data Exchange (ETDEWEB)

    Jung, H. J.; Kim, E. M.; Kim, B. C.; Kim, C. G [Wonkwang University Hospital, Iksan (Korea, Republic of)

    2004-07-01

    Transferrin receptors (Tf-R) were expressed on the surfaces of cancer cells. In cancer cells that are rapidly proliferating, transferrin receptors are upregulated due to the need for increasing non-hem iron to catalyze the first and rate-limiting step of DNA synthesis. Therefore transferrin (Tf) is thought to be a major ligand to find cancer cells. In this study, we investigated the targetability of Tf conjugated compounds to Tf-R expressed on cancer cells for detection and diagnosis and the usefulness of gamma probe-targeting delivery system on monitoring whether the gene complex bind to the cells specifically. For the detection and diagnosis of Tf-R positive cancer cells, Tf-chitosan conjugates were synthesized as previously described by Kircheis et al with some modifications. Succinimidyl 6-hydrazino nicotinate hydrochloride (HYNIC) was bound to Tf-chitosan conjugates. HYNlC-Tf-chitosan conjugates were labelled with 99m Tc. For tumor model, 5- to 6-week-old female BALB/c nude mice were injected subcutaneously in the left thigh with Ramos cells (human Burkitt's lymphoma). The gamma imagings were acquired after administration of Tc-99m Tf-chitosan conjugates. To compare the image acquired with HYNIC-Tf conjugate, Ga-67 study was performed. Radiolabeling yields of both Tf-chitosan conjugates was above 90% until 12hr. Uptake in the Ramos model of Tc-99m Tf-chitosan conjugate showed higher than those of Ga-67. A few minutes after injection Tc-99m Tf-chitosan conjugate localized mainly in the circulation (heart), kidneys, and tumor. At 24hr after injection, tumor to nontumor ratio of Ga-67 was approximately 4, but those of Tc-99m Tf-chitosan conjugate showed until 5 only 2 hr after injection. Using Tc-99m Tf-chitosan conjugate, transferrin binding to transferrin receptor on tumor cell could be seen in vivo.

  1. A combined procedure for sup(99m)Tc aerosol ventilation and perfusion imaging

    International Nuclear Information System (INIS)

    For several years, radioaerosols have been successfully used to provide detailed images of regional ventilation to aid in the differential diagnosis of pulmonary embolism. It has been widely advocated that the ventilation images should follow the perfusion scan and that the amount of aerosol deposited in the patient's lungs should be three times greater than the perfusion dose. We employed an alternative approach which avoided the deposition of an unpredictable amount of aerosol in individual patients. The aerosol study was performed first, and the activity of the microspheres used for the perfusion images was then tailored to the actual amount of aerosol which the patient had retained. This allowed a microsphere/aerosol activity ratio of 10:1 to be readily achieved, thus successfully masking the ventilation pattern by the perfusion activity. The faster biological clearance of sup(99m)Tc-DTPA aerosol from the lung fields, as compared to sup(99m)Tc-sulphur-colloid aerosol, allowed higher initial activities to be deposited in the lungs, thus enabling a high-resolution collimator to be used. When the perfusion study was delayed by 1 h (one effective half-life for the sup(99m)Tc-DTPA aerosol), it was not necessary to increase the perfusion activity required to mask the ventilation image. (orig.)

  2. Novel Tc-99m radiotracers for brain imaging

    Directory of Open Access Journals (Sweden)

    Alessandra Boschi

    2007-09-01

    Full Text Available A novel class of Tc-99m complexes able to cross the blood brain barrier has been investigated and described here. These compounds are formed by reacting the bis-substituted nitrido precusors [99mTc(N(PS2] (PS = phosphino-thiol ligand with triethylborane (BEt3 under strictly anhydrous conditions and using non-aqueous solvents. The molecular structure of these tracers was not fully established, but preliminary experimental evidence suggests that they result from the interaction of the Lewis base [99mTc(N(PS2] with the Lewis acid BEt3, which leads to the formation of the novel -B-Nº Tc- adduct. After purification and recovery in a physiological solution, the new borane-nitrido Tc-99m derivatives were injected in rats for evaluating their in vivo biological behavior. Results showed a significant accumulation in brain tissue, thus indicating that these complexes are capable of penetrating the intact blood brain barrier. Uptake in the central nervous system was confirmed by imaging the distribution of activity on the integrated living animal using a YAP(SSPECT small animal scanner.Uma nova classe de complexos de Tc-99m capazes de atravessar a barreira hemato-encefálica foi investigada e descrita neste trabalho. Estes compostos são formados reagindo os precursores bissubstituídos do nitrido [99mTc(N(PS2] (PS = phosphino-tiol ligante com trietillborano (BEt3 sob restritas condições anidras e usando solventes não aquosos. A estrutura molecular desses traçadores não foi totalmente estabelecida, mas evidências experimentais preliminares sugerem que eles resultam da interação da base de Lewis [99mTc(N(PS2] com o ácido de Lewis BEt3, levando a formação do novo aducto -B-Nº Tc-. Após purificação e recuperação em uma solução fisiológica, os novos derivados borano-nitrido-Tc-99m foram injetados em ratos para avaliação de seu comportamento biológico in vivo. Os resultados mostraram uma acumulação significativa no tecido cerebral

  3. Evaluation of usefulness of [sup 99m]Tc-GSA liver scintigraphy on hepatic ischemia-reperfusion injury in rats with obstructive jaundice

    Energy Technology Data Exchange (ETDEWEB)

    Gohbara, Hideo; Kohno, Yoshihiro; Sakae, Katsuyoshi; Sato, Shuhei; Shimizu, Mitsuharu; Takeda, Yoshihiro; Hiraki, Yoshio (Okayama Univ. (Japan). School of Medicine)

    1992-08-01

    [sup 99m]Tc-DTPA-galactosyl human serum albumin ([sup 99m]Tc-GSA) is a new liver-imaging agent which binds to hepatic binding protein. This study evaluated the sensitivity of [sup 99m]Tc-GSA kinetics and imaging anatomy to hepatic ischemia-reperfusion injury of obstructive jaundiced rats. Regional hepatic ischemia was induced by clamping the left hepatic artery and the left portal vein for 45 min. The blood clearance and hepatic accumulation indices were obtained on the basis of the dynamic data. A significant difference of these indices was observed between the ischemic group and the control. Marked depression in hepatic [sup 99m]Tc-GSA uptake was observed in the ischemic group. In conclusion, [sup 99m]tc-GSA is useful for evaluating the hepatic ischemia reperfusion injury in rats with obstructive jaundice. (author).

  4. Technetium-99m tetrofosmin imaging in thyroid diseases: comparison with Tc-99m-pertechnetate, thallium-201 and Tc-99m-methoxyisobutylisonitrile scans

    Energy Technology Data Exchange (ETDEWEB)

    Klain, M. [Centro di Studio per la Medicina Nucleare, Consiglio Nazionale delle Ricerche (CNR) Napoli (Italy)]|[Univ. di Napoli (Italy). Medicina Nucleare; Maurea, S. [Medicina Nucleare, Istituto Nazionale dei Tumori, Napoli (Italy); Cuocolo, A. [Centro di Studio per la Medicina Nucleare, Consiglio Nazionale delle Ricerche (CNR) Napoli (Italy)]|[Univ. di Napoli (Italy). Medicina Nucleare; Colao, A. [Endocrinologia, Centro di Studio per la Medicina Nucleare del Consiglio Nazionale delle Ricerche (CNR), Napoli (Italy)]|[Univ. di Napoli (Italy); Marzano, L. [Endocrinologia, Centro di Studio per la Medicina Nucleare del Consiglio Nazionale delle Ricerche (CNR), Napoli (Italy)]|[Univ. di Napoli (Italy); Lombardi, G. [Endocrinologia, Centro di Studio per la Medicina Nucleare del Consiglio Nazionale delle Ricerche (CNR), Napoli (Italy)]|[Univ. di Napoli (Italy); Salvatore, M. [Centro di Studio per la Medicina Nucleare, Consiglio Nazionale delle Ricerche (CNR) Napoli (Italy)]|[Univ. di Napoli (Italy). Medicina Nucleare

    1996-12-01

    In this study, tetrofosmin whole-body scintigraphy was performed in 35 patients with evidence of thyroid diseases. All patients underwent laboratory evaluation of thyroid function as well as {sup 99m}Tc pertechnetate scan, thallium-201 {sup 99m}Tc-methoxyisobutylisonitrile (MIBI) whole-body studies. Thyroid images were semi-quantitatively analysed by a 4-point score: 0=no significant uptake; 1=uptake increased but inferior to normal thyroid tissue; 2=uptake equal to normal thyroid tissue; 3=uptake superior to normal thyroid tissue. A total of 41 thyroid nodules were detected, of which 15 were goitre nodules, 13 adenomas and 13 malignant lesions. In goitre nodules, concordant results of tetrofosmin and pertechnetate uptake (score 1 or 0) were observed in the majority of lesions (87%). In function adenomas both tetrofosmin uptake and pertechnetate uptake were score 3. In non-function adenomas tetrofosmin uptake was score 3, while pertechnetate uptake was score 0. In six malignant lesions, tetrofosmin uptake was score 3, while pertechnetate uptake was score 0; in the other seven lesions, where a prevalence of goitre abnormalities was observed, results of tetrofosmin and pertechnetate uptake were similar (score 0 or 1). In (70%) patients with malignant nodules, whole-body tetrofosmin images showed increased abnormal uptake in a total of 28 extra-thyroid tumour sites, as subsequently confirmed by other techniques. When tetrofosmin images were compared to {sup 201}Tl and {sup 99m}Tc-MIBI scans, concordant results were observed in all cases. In conclusion, tetrofosmin imaging may be particularly useful to characterize and stage patients with malignant thyroid nodules; it shows similar results to thallium but provides better image quality. Comparable findings were observed between tetrofosmin and MIBI studies. Thus, tetrofosmin may be an alternative to thallium and MIBI in the aforementioned patients. (orig.). With 4 figs., 3 tabs.

  5. Technetium-99m sestamibi: an indicator of breast cancer invasiveness

    International Nuclear Information System (INIS)

    As recently shown, angiogenesis is the most reliable marker of breast cancer invasiveness. Unfortunately it must be assessed by immunohistochemistry on tissue specimens. We have used technetium-99m sestamibi, a marker of regional blood flow in other organs that often but not always images breast cancer, to assess the invasiveness of this tumour. Nineteen patients, ten with nodal metastases and nine without any metastases, were studied with 99mTc-sestamibi scintigraphy before operation. Angiogenesis was quantitatively assessed by immunohistochemical staining of endothelia for factor VIII. All the node-positive (N+) patients at surgical revesion showed a positive 99mTc-sestamibi scan of the primary tumour and all the N-patients were negative. Nine out of ten N+ and sestamibi-positive tumours showed more than 135 microvessels/mm2 and one showed 99 microvessels/mm2; by contrast there were 71.6±12.1 microvessels/mm2 in the nine N- and sestamibi-negative tumours. Our study suggests that 99mTc-sestamibi is a marker of breast cancer invasiveness: its uptake is related to angiogenesis and, possibly, to oxidative metabolism of the tumour. (orig.)

  6. Technetium-99m sestamibi: an indicator of breast cancer invasiveness

    Energy Technology Data Exchange (ETDEWEB)

    Scopinaro, F. (Section of Nuclear Medicine, Dept. of Experimental Medicine, Univ. ' La Sapienza' , Rome (Italy)); Schillaci, O. (Section of Nuclear Medicine, Dept. of Experimental Medicine, Univ. ' La Sapienza' , Rome (Italy)); Scarpini, M. (1st Inst. of Surgery, Univ. ' La Sapienza' , Rome (Italy)); Mingazzini, P.L. (1st Inst. of Surgery, Univ. ' La Sapienza' , Rome (Italy)); Di Macio, L. (Section of Nuclear Medicine, Dept. of Experimental Medicine, Univ. ' La Sapienza' , Rome (Italy)); Banci, M. (Section of Nuclear Medicine, Dept. of Experimental Medicine, Univ. ' La Sapienza' , Rome (Italy)); Danieli, R. (Section of Nuclear Medicine, Dept. of Experimental Medicine, Univ. ' La Sapienza' , Rome (Italy)); Zerilli, M. (1st Inst. of Surgery, Univ. ' La Sapienza' , Rome (Italy)); Limiti, M.R. (1st Inst. of Surgery, Univ. ' La Sapienza' , Rome (Italy)); Centi Colella, A. (Section of Nuclear Medicine, Dept. of Experimental Medicine, Univ. ' La Sapienza' , Rome (Italy))

    1994-09-01

    As recently shown, angiogenesis is the most reliable marker of breast cancer invasiveness. Unfortunately it must be assessed by immunohistochemistry on tissue specimens. We have used technetium-99m sestamibi, a marker of regional blood flow in other organs that often but not always images breast cancer, to assess the invasiveness of this tumour. Nineteen patients, ten with nodal metastases and nine without any metastases, were studied with [sup 99m]Tc-sestamibi scintigraphy before operation. Angiogenesis was quantitatively assessed by immunohistochemical staining of endothelia for factor VIII. All the node-positive (N+) patients at surgical revesion showed a positive [sup 99m]Tc-sestamibi scan of the primary tumour and all the N-patients were negative. Nine out of ten N+ and sestamibi-positive tumours showed more than 135 microvessels/mm[sup 2] and one showed 99 microvessels/mm[sup 2]; by contrast there were 71.6[+-]12.1 microvessels/mm[sup 2] in the nine N- and sestamibi-negative tumours. Our study suggests that [sup 99m]Tc-sestamibi is a marker of breast cancer invasiveness: its uptake is related to angiogenesis and, possibly, to oxidative metabolism of the tumour. (orig.)

  7. Technetium-99m Radiopharmaceuticals in Neurology. Chapter 6

    International Nuclear Information System (INIS)

    The ideal radioisotope for single photon emission computed tomography imaging is 99mTc, due to its physical decay characteristics, its availability through commercially available generator systems and its low cost per dose. Technetium-99m hydrophilic complexes are used to evaluate the integrity of the blood-brain barrier, while neutral and lipophilic complexes are used as brain perfusion imaging agents for determination of changes in regional cerebral blood flow in various neurological disorders. Radiopharmaceuticals that bind to central nervous system (CNS) receptors in vivo are useful for understanding the pathophysiology of a number of neurological and psychiatric disorders, their diagnosis and treatment. Nowadays, CNS receptor imaging agents are, with some exceptions, typically positron emission tomography radionuclide based radiopharmaceuticals. The reason for this is not based on principal but is rather as a result of the fact that efforts in the direction of 99mTc containing agents have not been strong or consistent enough. In the chapter, the progress made in the development of 99mTc complexes for imaging dopamine transporter, 5-HT1A receptor and amyloid plaques is presented. (author)

  8. Osteomyelitis diagnosis by {sup 99m}Tc radiolabeled aptamers

    Energy Technology Data Exchange (ETDEWEB)

    Santos, S.R.; Ferreira, I.M.; Andrade, A.S.R., E-mail: sararoberta7@hotmail.com, E-mail: imendesf@yahoo.com.br, E-mail: antero@cdtn.br [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Barros, A.L.B.; Cardoso, V.N.; Diniz, O.F., E-mail: brancodebarros@yahoo.com.br, E-mail: valbertcardoso@yahoo.com.br, E-mail: simoneodilia@yahoo.com.br [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil). Faculdade de Farmacia. Departamento de Analises Clinicas e Toxicologicas

    2015-07-01

    Osteomyelitis, which is characterized by progressive inflammatory destruction and new opposition of bone, is still a difficult infection to treat. The clinical diagnosis in late stages is achieved easily, but an early diagnosis is more challenging. Staphylococcus aureus is a common agent found in osteomyelitis and bone prostheses infection. Diagnosis by scintigraphy has advantages because it is a non-invasive procedure and is able to perform an early diagnosis even before anatomic changes. Thus, nuclear medicine could contribute to an accurate diagnosis since specific radiopharmaceuticals were developed. In this study, aptamers selected to Staphylococcus aureus were labeled with {sup 99m}Tc and used for bacteria identification in an osteomyelitis experimental model. The aptamers selected to S. aureus were directly labelled with {sup 99m}Tc and were evaluated by biodistribution studies. Wistar rats with intraosseous infection in the right paw were used. A random aptamer labelled with {sup 99m}Tc was as control. Six animals were used in each group. The aptamers labeled with {sup 99m}Tc were able to identify the infection foci caused by S. aureus displaying a target/non-target ratio of 2,23 ± 0,20, after 3 h. The control group presented a target/non-target ratio 1,08 ± 0.23. The results indicated that the radiolabeled aptamers were able to identify specifically the infection foci and they should be further explored for infection diagnosis by scintigraphy. (author)

  9. Preparation and quality control of fibrinogen 99mTc

    International Nuclear Information System (INIS)

    A method of fibrinogen preparation is presented in order to label it with 99mTc employing Sn as reducing agent in alkaline medium. Purity controls by chromatography, coagulation in rabbits and biodistribution in rats were performed. It is concluded that optimal time incubation is between 22 and 23 hs. (M.E.L.)

  10. 99m Tc-labeled heparin test in orthopaedic surgery

    Energy Technology Data Exchange (ETDEWEB)

    Bouvier, J.F.; Lafon, J.C.; Colin, M.; Chatelut, J.; Beaubatie, F. (Hopital Universitaire Dupuytren, Limoges (France))

    1983-06-30

    99m Tc-labeled heparin test was performed for early detection of phlebitis or pulmonary embolism after orthopaedic prothesis. Heparinic treatment and surgery per se were demonstrated to have no effect on the results. If this test demonstrates a statistical difference for pathologic patients, it is of greater value to consider ratio between rates before and after intervention.

  11. Osteomyelitis diagnosis by 99mTc radiolabeled aptamers

    International Nuclear Information System (INIS)

    Osteomyelitis, which is characterized by progressive inflammatory destruction and new opposition of bone, is still a difficult infection to treat. The clinical diagnosis in late stages is achieved easily, but an early diagnosis is more challenging. Staphylococcus aureus is a common agent found in osteomyelitis and bone prostheses infection. Diagnosis by scintigraphy has advantages because it is a non-invasive procedure and is able to perform an early diagnosis even before anatomic changes. Thus, nuclear medicine could contribute to an accurate diagnosis since specific radiopharmaceuticals were developed. In this study, aptamers selected to Staphylococcus aureus were labeled with 99mTc and used for bacteria identification in an osteomyelitis experimental model. The aptamers selected to S. aureus were directly labelled with 99mTc and were evaluated by biodistribution studies. Wistar rats with intraosseous infection in the right paw were used. A random aptamer labelled with 99mTc was as control. Six animals were used in each group. The aptamers labeled with 99mTc were able to identify the infection foci caused by S. aureus displaying a target/non-target ratio of 2,23 ± 0,20, after 3 h. The control group presented a target/non-target ratio 1,08 ± 0.23. The results indicated that the radiolabeled aptamers were able to identify specifically the infection foci and they should be further explored for infection diagnosis by scintigraphy. (author)

  12. [Complementary scintigraphic examinations (99mTc-MDP, 99mTc-MIBI and Octreoscan) in breast neoplasm assessment].

    Science.gov (United States)

    Stefănescu, C; Chossiere, L; Rusu, V; Meignan, M

    1996-01-01

    Scintigraphic imagery was used in breast carcinoma mostly for the bone metastases screening, using 99mTc-MDP. Others radiopharmaceuticals can be used to visualize either the primary tumour or the soft tissues metastases. It's mainly the case of 99mTc-MIBI, and also of some somatostatin analogues, like Octreoscan. This case report of a patient with a T3N1M+ breast adenocarcinoma having different secondary sites represent also a prove of the complementarity of these explorations, which give the possibility of diagnostic and therapeutic evaluation and, in the same time, the possibility of a better understood of the metastasizing mechanism. PMID:9455458

  13. Revision: quality control of {sup 99m}Tc; Revisao: controle da qualidade do {sup 99m}Tc

    Energy Technology Data Exchange (ETDEWEB)

    Alves, S.M.; Kabad, M.C. [Universidade Estacio de Sa (UESA), Rio de Janeiro, RJ (Brazil); Teixeira, G.J.; Sousa, C H.S., E-mail: sanny.alves@yahoo.com.br [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

    2015-07-01

    The radiopharmaceutical most commonly used in nuclear medicine is technetium-99m. Radiopharmaceuticals labeled with {sup 99m}Tc are the main agents for diagnosis, being used in the vast majority of procedures due to radionuclide characteristics. During its preparation impurities can be generated that make products with different qualities of the desired. In this paper were presented some of the factors that can interfere with radiopharmaceuticals and the tests used for quality assurance, certifying that the product is fit for purpose. It is up to nuclear medicine centers deploy a program to raise the quality of exams and increase patient safety. (author)

  14. Poor Tc-99m dimercaptosuccinic acid uptake, re-evaluation with Tc-99m MAG3 scintigraphy in Lowe syndrome

    Science.gov (United States)

    Koca, Gokhan; Atilgan, Hasan Ikbal; Demirel, Koray; Diri, Akif; Korkmaz, Meliha

    2011-01-01

    Tc-99m dimercaptosuccinic acid (DMSA) is filtered through the glomeruli and reabsorbed by the proximal tubules as low molecular weight proteins. In Lowe syndrome this mechanism is impaired and so poor DMSA uptake is seen. Poor DMSA uptake was shown in very few studies, but none mentioned normal Tc-99m MAG3 uptake. In this case, the patient had poor DMSA uptake, normal MAG3 uptake and a neurogenic bladder in anterior to the left kidney that attenuates left kidney. PMID:23559713

  15. The radio-labeling of Ciprofloxacin with Technetium-99m

    International Nuclear Information System (INIS)

    Even with rapid technological development in the field of diagnostic imaging, the localization of infection continues to pose challenges in day-to-day routine clinical practice. Tc-99m Ciprofloxacin is a relatively new radiopharmaceutical, which has proven its utility in imaging infection. This paper presents a new method of labeling Ciprofloxacin with Tc-99m using SnCI2.2H20 as reducing agent. The procedure used 2 mg of Ciprofloxacin manufactured by Bayer, 400 μg of SnCI2.2H20 (Sigma Chemical Co.) and 185MBq of Technetium-99m in the form of pertechnetate (Tc-99mO4) in a volume of 300 μl. The labeling was carried out at 100 deg. C for 10 minutes and at ambient temperature for a similar period of time. The solution obtained was filtered using millipore filter of 0.22 μm size. The efficiency of the labeling, verified by ascending chromatography on Whatman No.1 paper was found to be 97.3 % (±1.6), while it was 96.8% (±2.3) using Whatman No.3 paper and 96.6% (± 2.1) using thin-layer chromatography. Chromatography by exclusion chromatography (Bio-Gel P 10) was used for confirmation of the above results. The labeled molecules were eluted first, followed by the molecules of Technetium-99m while the colloids remained attached to the column. The results of the present study are comparable with the results of previous studies reported in literature. (author)

  16. Production technologies for molybdenum-99 and technetium-99m

    International Nuclear Information System (INIS)

    Technetium-99m (6.02 h) is the most widely used radioisotope in nuclear medicine, accounting for more than 80% of all diagnostic nuclear medicine procedures. It is almost exclusively produced from the decay of its parent 99Mo. The present sources of 99Mo are research reactors by using the (n,γ) nuclear reaction with natural Mo (98Mo, ∼24%), resulting in inexpensive but low-specific activity 99Mo, or by neutron-induced fission of 235U, which results in expensive but high specific activity 99Mo. This publication covers several aspects related to the production of 99Mo and 99mTc. The contributed papers reflect the current status of the technology and discuss potential alternative methodologies for the production of 99Mo and 99mTc for medical use. The first four papers address the technologies using nuclear reactors, including the description of a new method using an aqueous homogenous reactor core for production of fission 99Mo and the latest development efforts to fabricate 235U low enriched targets (LEU, 235U). The next five papers discuss the potential of utilizing particle accelerators and assess the current status of the available nuclear data for the production of both, 99Mo and 99mTc with proton and deuteron beams. The last paper discusses a new technology based on gel system for the preparation of 99Mo/99mTc generators using low specific activity 99Mo produced in research reactors by the neutron activation of natural and inexpensive molybdenum oxide targets. Each individual paper was indexed and abstracted

  17. Quantitative interpretation method for detection of head and neck cancer with Tc-99m MIBI SPECT

    Energy Technology Data Exchange (ETDEWEB)

    Seok, Ju Won; Kim, In Ju; Kim, Yong Ki; Wang, Soo Geun [College of Medicine, Pusan National Univ., Pusan (Korea, Republic of)

    2001-07-01

    The aim of this study was to present a method for diagnosis of head and neck cancer with Tc-99m MIBI SPECT by creating regions of interest (ROIs). Tc-99m MIBI SPECT was performed at 10 minutes after injection of 750 MBq of Tc-99m MIBI on 42 patients (36 males, 6 females. mean age=59.1yr) with the clinical suspicion for head and neck tumors. All lesions were histopathologically proven and data was analysed by creating ROIs over lesions and various backgrounds; submandibular gland (L/S), parotid gland (L/P). nuchal muscle (L/N), scalp muscle (L/Se). Each lesion to background ratios were calculated for each patients. Malignant disease were 37 patients and benign lesions were 5 patients, there was no statistically significant correlation between the histopathologic results and L/S ratio (p>0.05). L/P, L/N. L/Sc ratios of malignant disease and benign lesion revealed statistical difference (p<0.05). When ROC curve analysis was used, Area under the ROC curve of L/Sc ratio was largest. Sensitivity and specificity of MIBI SPECT using L/Sc ratio were 100% and 100%, respectively. It is more helpful and objective method appling L/Sc ratio to differentiating malignant from benign lesions in head and neck.

  18. Evaluation of RI images of hepatic blood flow using Tc-99m PMT

    Energy Technology Data Exchange (ETDEWEB)

    Fujiwara, Hiromichi; Iwasaki, Naoya; Ichikawa, Kesato

    1988-03-01

    To evaluate the clinical significance of RI images of hepatic blood flow using Tc-99m-PMT, analysis of the RI images and estimation of hepatic blood flow were carried out in patients with various liver diseases (37 cases). After intravenous injection of Tc-99m-PMT, hepatic accumulation curve of ROI positioned at whole liver area and time activity curve of ROI positioned at celiac artery were obtained through scintillation camera images with the computer-analysed system. Hepatic blood flow coefficient (K) was calculated from the hepatic accumulation curve. Based on the differential curve calculated from the time activity curve, chronological images of arterial, portal, parenchymal and saturated parenchymal phases were obtained. Results ; 1) K was 0.50 + 0.04, 0.35 + 0.02 and 0.26 + 0.04/min in normal type, CH type and LC type respectively. These coefficients well correlated with clinical severity of hepatic diseases. 2) Perfusional phase images of the liver became poorer in accordance with progression of liver disease, while images of spleen, portal venous system and collateral channels were more clearly obtained in liver cirrhosis. This method was shown to have a potential to understanding of severity of liver disease and hepatic blood flow dynamics.

  19. [A new lymph node imaging agent--99mTc-polyphase liposome oleatis (99mTc-plo)].

    Science.gov (United States)

    Yu, B F

    1988-07-01

    A new lymph node radio-imaging agent, technetium-99m polyphase liposome oleatis (99mTc-plo), has recently been developed. Polyphase liposome oleatis was labelled with radionuclide by stannous chloride method. The labelled rate was 90% or more as technically identified by thin layer chromatography, external gamma-camera imaging and radioautography. In animal experiment, 0.2-0.3 ml (0.2 mci) of the 99mTc-plo was injected subcutaneously into the toes web of rats. After half an hour, the regional lymph nodes of popliteal fossa were visualized very clearly. The imaging figures may keep their distinct shadow up to 24 hours. The tested rabbits were sacrificed 10 hours after 99mTc-plo injection for detecting various kinds of tissue and organ with a scintillation counter. The regional lymph nodes revealed the highest uptake rate of the new agent, 12,116; 1,303; 1,615 times higher than that of the adjacent muscles, liver and spleen, respectively. In clinical experiment, 0.2-0.3 ml (0.5-0.8 mci) of the 99mTc-plo was injected subcutaneously into the toes web of patients. Half an hour later, the lymph nodes of inguinal, external iliac and common iliac regions appeared in sequence. If the new agent is injected perianally, the internal iliac lymph nodes will be seen. No side effect was observed in both types of experiment. This new agent has been tried in rats, rabbits and dogs with similar positive results. The new lymph node imaging technique is simple, safe, reliable and reproducible. This agent, being directed toward the lymph nodes and possessing affinity to cancer cells, is expected to be supplementary method to CT and B-ultrasonography for detecting lymphoid malignancy and lymph node metastasis. PMID:3248482

  20. Perfusion lymphoscintigraphy using sup 99m Tc-human serum albumin in patients with treated uterine cancer

    Energy Technology Data Exchange (ETDEWEB)

    Kataoka, Masaaki; Hamada, Katsuyuki; Hamamoto, Ken; Takeda, Yasunari; Matsuura, Shumpei (Ehime Univ., Matsuyama (Japan). School of Medicine); Kawamura, Masashi

    1990-09-01

    Perfusion lymphoscintigraphy was performed by subcutaneous injection of 7.4 MBq (0.2mCi) {sup 99m}Tc-human serum albumin ({sup 99m}Tc-HSA) on 18 patients with uterine cancer treated by operation and/or irradiation. Radioactivity at the injection site was counted for 3 min at 10 min (a) and at 3 hr (b) after injection, and the clearance of {sup 99m}Tc-HSA was defined as (1-(b)/(a)) x 100(%) ((a) and (b) were corrected for decay of the isotope). The clearance in 6 legs with lymphedema was significantly more delayed than that in 16 legs without lymphedema in the patients treated with both surgery and irradiation (16.6 +- 7.7% vs 34.9 +- 9.3%: P< 0.01). The clearances in edematous legs in a case which had developed a venous occulusion after operation were 44.2% and 41.7%, which were almost the same as those in the non-edematous patients treated with surgery alone. The clearance in patients treated with both operation and irradiation was significantly more delayed than that in patients treated with the signle modality of operation or irradiation (29.7 +- 11.9% vs 41.1 +- 7.2%, 44.5 +- 7.7%, respectively: P< 0.01). These data suggest that perfusion lymphoscintigraphy using {sup 99m}Tc-HSA is useful for evaluating patients with lymphedema and for differentiating it from edema caused by other mechanisms. (author).

  1. 99m-technetium tetrofosmin and 99mTc-methylene diphosphonate in pre-surgical breast cancer

    International Nuclear Information System (INIS)

    Full text: The aim of this study was to assess the role of the Tc-99m Tetrofosmin (TF) in conjunction with Tc-99m methylenediphosphonate (MDP) in pre-surgical breast cancer (BC) staging. Thirty-six female patients, age range was 32-70 years (average 51.45 years), where the clinical examination, mammography (MG) and fine-needle aspiration cytology were inconclusive were subjected to Tc-99m TF Scintimammography (SMM). All the patients were examined in a specialized breast clinic by experienced surgeons and radiologists. Of the 36 patients, 24 had hyperdense breasts, 8 had undergone lumpectomy or mastectomy due to cancer in one of the breasts and were included in the present study because of suspicion of lesion in the contra lateral breast, 2 had palpable axillary lymph nodes (LN) but no palpable breast lump and 2 patients were of mastitis carcinomatosis. All biopsies were histopathologically verified 740 MBq of Tc-99m TF (Myoview-Amersham) was injected in the arm opposite to the side of the breast lesion. In patients with bilateral breast lesions (BL) radiopharmaceutical was injected in a pedal vein. Planar imaging in prone position was done 10-15 min later. Two lateral views of the left and right breasts including axilla were acquired followed by an anterior view in supine position with arm in an upright position so as to include both breasts and axillary region in the field of imaging view. Imaging was done using a large field of view single-head gamma camera (Diacam-Siemens) coupled with low-energy high-resolution collimator. In patients with locally advanced BC who were to receive pre-operative neo-adjuvant chemotherapy, cardiac GATED SPECT was also acquired using standard protocol. After few days, Tc-99m MDM scintimammography and whole-body bone scintigraphy was also performed. SMM, using the same acquisition protocol as with 99mTc-TF, was done 5-10 min after intravenous injection of 555-740 MBq 99mTc MDP. Standard WBBS was acquired two to three hours later. SMM

  2. The single-plasma-sample method for determining the glomerular filtration rate with Tc-99m-diethylenetriamine pentaacetic acid in childhood and adolescence. Is it age-dependent?

    Energy Technology Data Exchange (ETDEWEB)

    Itoh, Kazuo [JR Sapporo General Hospital (Japan); Matsuyama, Takeshi [Tokyo Metropolitan National Health Insurance Organization Fussa Hospital (Japan)

    2002-12-01

    The aim of this study is to assess the accuracy of the pre-existing single-plasma-sample method (SPSM) to measure the glomerular filtration rate (GFR) with Tc-99m-diethylenetriamine pentaacetic acid (Tc-99m-DTPA) in children and adolescents. In addition, the age-independent SPSM is evaluated with two algorithms (Bubeck and Russell) applied for Tc-99m-mercaptoacetyltriglycine (Tc-99m-MAG3) SPSM. The study was performed on 14 patients (12 men and 2 women; age range 3 to 19 yr) with renal diseases. Tc-99m-DTPA (5 MBq/kg) was injected intravenously and thereafter blood samples were taken at 5, 15, 60, 90, 120, 150 and 180 min via the indwelling tube. Radioactivity in the injection syringe and plasma was measured by means of a double-well single-plastic scintillation counter. The true'' GFR as a reference was determined by two methods: 2-exponential curve fitting 7 samples (GFR{sub 7}) and 1-exponential curve fitting 3 samples between 90 and 150 min (GFR{sub 3}) in a slow clearance phase. The GFR{sub 7} and GFR{sub 3} were searched for to the clearance (GFR{sub 1}) estimated from a plasma concentration at various sample times by means of 3 equations designed for children (Groth and Aasted, Ham-I and -II) and 3 for adults (Christensen and Groth, Jacobsson, Itoh). All the SPSM showed close correlations (r>0.95) with the reference methods. Among them, Jacobsson's equation at sample time=120 min tended to be the most accurate (r=0.9826, root mean squared error (RMSE)=7.8 ml/min). On the other hand, Ham-I's equation at sample time=120 min was the most accurate, when it was referred to GFR{sub 3} in correction for overestimation (r=0.9951, RMSE=4.60 ml/min). The Bubeck and Russells' algorithms showed that the regression equation between the GFR{sub 7} and the estimates was different in 2 groups of adults (49 cases) and children/adolescents. Our study indicates that Jacobsson's and Christensen and Groth's equations designed for adults are

  3. Tc-99m ciprofioxacin imaging in acute cholecystitis

    Institute of Scientific and Technical Information of China (English)

    Yun-Mee Choe; Kee-Chun Hong; Seok-Hwan Shin; Kyung-Rae Kim; Ze-Hong Woo; Wonsick Choe; Keon-Young Lee; Seung-Ik Ahn; Kwang Kim; Young Up Cho; Sun Keun Choi; Yoon-Seok Hur; Sei-Joong Kim

    2007-01-01

    AIM: To evaluate the efficacy of a new nuclear imaging Infecton (Tc-99m ciprofioxacin) for the diagnosis of acute cholecystitis.METHODS: Sixteen patients thought to have acute cholecystitis were included in this study. The diagnosis of acute cholecystitis was made based on clinical symptoms and ultrasonographic and pathologic findings.RESULTS: The 16 patients were composed of 12 acute and 4 chronic cholecystitis patients. Twelve patients with acute cholecystitis were image-positive, including one false-positive. Four patients with chronic cholecystitis were image-negative, of whom three were true-negative.This nuclear imaging had a sensitivity of 91.7%, a specificity of 75%, a positive-predictive value of 91.7%,and a negative-predictive value of 75%.CONCLUSION: Tc-99m ciprofloxacin imaging is easy to perform and applicable for the diagnosis of acute cholecystitis.

  4. Dynamic 99mTc-DTPA radioaerosol lung scanning for the evaluation of alveolar-capillary barrier permeability

    International Nuclear Information System (INIS)

    Pulmonary clearance of small droplet 99mTc-DTPA radioaerosol was studied in 100 patients (12 normal subjects, N; 10 asymptomatic healthy smoker, FA; 31 patients with interstitial lung diseases, IP; 47 patients with chronic obstructive lung disease, BPCO). The first seven minutes of clearance were described with the function At=Ao*exp(-K*t) and the time constant K was considered representative of the 99mTc-DTPA clearance rate and hence of the alveolar-capillary barrier permeability. Groups FA, IP and BPCO showed a significant (p99mTc-DTPA dynamic lung scanning is an easy, non-invasive method to assess derangements of alveolar-capillary barrier permeability secondary to epithelial damage; 2) permeability increase is a very early effect of cigarette smoke damafe to the epithelium; 3) other mechanisms of epithelial injury are present in diffuse lung disease; 4) while the clinical role of this new pathophysiological test is not yet clear, it is likely that it may become a very early marker of pulmonary epithelial damage in diffuse lung disease

  5. Tumor scintigraphy with sup(99m)Tc-tetracycline

    International Nuclear Information System (INIS)

    Of all radiopharmaceuticals, sup(99m)Tc-tetracycline has turned out to be the most efficient and to have a specific affinity for tumours. Due to its favourable physical properties, high doses can be given at relatively low radiation loads. Its pharmacological properties ensure that it is quickly eliminated from the rest of the body during enrichment in affinite tissue. Furthermore, it can be labelled at any time with an economical radionuclide. (GSE/AK)

  6. Tc99m-sestamibi dosimetry in myocardial perfusion imaging

    International Nuclear Information System (INIS)

    This paper addressed myocardial perfusion imaging providing a spatial dosimetric investigation of the 99mTc-radiopharmaceutical dose distribution at the myocardium. Radiological data manipulation was performed in order to create a computational voxel model of the heart. A set of images obtained by thoracic angiotomography and abdominal aorta was set up providing anatomic and functional information for heart modeling in SISCODES code. A homogeneous distribution of 99mTc was assumed into the cardiac muscle. Simulations of the transport of particles through the voxel and the interaction with the heart tissues were performed on the MCNP - Monte Carlo Code. The spatial dose distribution in the heart model is displayed as well as the dose versus volume histogram of the heart muscle. The present computational tools can generate spatial doses distribution in myocardial perfusion imaging. Specially, the dosimetry performed elucidates imparted dose distribution in the myocardial muscle per unit of injected 99m Tc activity, which can contribute to future deterministic effect investigations. (author)

  7. Technetium-99m in nuclear medicine and radiation protection experience

    Energy Technology Data Exchange (ETDEWEB)

    Ninkovic, M. M.; Raicevic, J. J.; Pavlovic, S.; Glisic, R. [Institute of Nuclear Sciences, Belgrade, Yugoslavia (Yugoslavia)

    2002-07-01

    The one of the major contributors to personnel exposure whithin nuclear medicine laboratories is primarily now ubiquitous {sup 99}Mo-{sup 99m}Tc generators. These generators, first available about more than forthy years ago, are now providing large quantities of {sup 99m} Tc radioactive source daily in the most laboratories, both for direct injection, as a per-technetate ion, and through the utilization of the kits for the preparation of dipherent radiopharmaceuticals. The safe and efficient utilization of technetium-99m in nuclear medicine, as other radionuclides, begin with the design, construction and operation of the 'Hot'Laboratory area, where radionuclide are stored and patient doses are prepared. Regardless of the specific physical configuration, suitable facilities need to be provided and good working practices established in order to get these principal objectives: (a) keep radiation exposure to personnel at ALARA levels; (b) prevent contamination of personnel; (c) prevent contamination of measuring instruments (including imaging devices such as gamma cameras and rectilinear scanners), and (d) prevent spread of contamination to places or persons outside the laboratory.

  8. Preparation and in vivo biological investigations on a novel radioligand for bone scanning: technetium-99m-labeled zoledronic acid derivative

    Energy Technology Data Exchange (ETDEWEB)

    Lin Jianguo; Qiu Ling, E-mail: qiulingwx@gmail.com; Cheng Wen; Luo Shineng; Ye Wanzhong

    2011-07-15

    Introduction: To enable imaging at an earlier time after injection, a radiopharmaceutical with higher affinity for bone, larger ratio of bone-to-soft tissue uptake and more rapid clearance from blood is required. The nature of diphosphonic acid is a key factor to determine the advantages of the radiopharmaceuticals. The purpose of this study is to optimize the linker chain between the imidazolyl and geminal diphosphonate group in the zoledronic acid (ZL) to develop novel single photon emission computed tomography (SPECT) bone imaging agent. Methods: A novel ZL derivative, 1-hydroxy-3-(1H-imidazol-1-yl)propane-1,1-diyldiphosphonic acid (IPrDP), was successfully prepared and labeled with {sup 99m}Tc in a high labeling yield. Biodistribution of {sup 99m}Tc-IPrDP and {sup 99m}Tc-ZL in normal mice were studied and compared. SPECT bone scanning was performed on the rabbit and a series of dynamic and static images were recorded by Philips SKY Light emission computed tomography. Results: In the biodistribution studies, {sup 99m}Tc-IPrDP exhibits significant advantages on the bone resorption and the clearance from soft tissues compared with {sup 99m}Tc-ZL. Kinetics of blood clearance in mice showed that T{sub 1/2{alpha}} and T{sub 1/2{beta}} of {sup 99m}Tc-IPrDP were 1.47 min and 46.47 min, while those of {sup 99m}Tc-ZL were 2.28 and 52.63 min respectively. Excellent images of the rabbit skeleton can be quickly obtained for {sup 99m}Tc-IPrDP, which was faster than {sup 99m}Tc-ZL and the clinically widely used bone imaging agent {sup 99m}Tc-MDP (technetium-99m labeled with methylenediphosphonate). Conclusions: {sup 99m}Tc-IPrDP possesses excellent characteristics for the potential application as a novel bone scanning agent.

  9. TOPOLOGY AND VORTEX STRUCTURES OF A CURVING TURBINE CASCADE WITH TIP CLEARANCE ( Ⅰ )- EXPERIMENTAL MODEL AND TOPOLOGICAL FLOW PATTERNS ON BOTH ENDWALLS AND BLADE SURFACES

    Institute of Scientific and Technical Information of China (English)

    杨庆海; 黄洪雁; 韩万今

    2002-01-01

    By means of ink trace visualization of the flows in conventional straight, positively curved and negatively curved cascades with tip clearance, and measurement of the aerodynamic parameters in the transverse section, and by appling topology theory, the structures on both endwalls and blade surfaces were analyzed. Compared with conventional straight cascade, blade positive curving eliminates the separation line of the upper passage vortex and leads the secondary vortex to change from close separation to open separation,while blade negative curving effects merely the positions of singular points and the intensities and scales of vortex.

  10. Evaluation of '99Mo presence in eluates of 99mTc used in nuclear medicine

    International Nuclear Information System (INIS)

    Tc-99m is used for diagnostic imaging in nuclear medicine through SPECT technique. It is obtained by the elution of 99Mo/99mTc generators. During the elution process 99Mo can be extracted becoming a radionuclidic impurity. One of the quality parameters of the eluate is the radionuclidic purity, MBT (molybdenum break through), defined as the ratio between 99Mo and 99mTc activities in the eluate. The North-American and European pharmacopoeias restrict the 99Mo content, respectively, in 0.015 e 0.1% and, according to the International Atomic Energy Agency (IAEA), the activity ratio at the moment of administration of the radiopharmaceutical to the patient, should not exceed 0.015%. In Brazil, the control of such parameter is not obliged in official regulations. Thus, the objective of this work is to evaluate the occurrence of 99Mo in 99mTc eluates. It was initially optimized a methodology to determine the activity of 99Mo in eluate samples. Efficiency curves were obtained for a NaI (Tl) 8'' x 4'' scintillation detector installed at the In Vivo Monitoring Laboratory (LABMIV-IRD). The methodology has been validated through the measurement of a 99Mo standard liquid source calibrated at the National Metrology Laboratory for Ionizing Radiation (LNMRI-IRD). The samples analyzed in this work were gently supplied by 5 Nuclear Medicine Clinics located in the city of Rio de Janeiro. The activities of 99mTc and 99Mo in those samples have been measured respectively at the clinics and at the LABMIV. By applying a standardized methodology, the ration between the activities were calculated. The results show that 147 out of 174 samples presented 99Mo activities above the minimum detectable activity of the technique. On the other hand, only 2 out of 147 samples surpassed the MBT limit suggested by the IAEA and have been detected in samples eluted from generators of 750 mCi. In one of the samples, eluted from a generator of 1000 mCi, the calculated MBT value was very close to the IAEA

  11. Comparison of measurements of plasma clearance of Mebrofenine and of indocyanine green; Comparaison des mesures de clairance plasmatique de la mebrofenine et du vert d`indocyanine

    Energy Technology Data Exchange (ETDEWEB)

    Maurel, G.; Coutris, G. [Service Central de Medecine Nucleaire, Hopital Saint Antoine (France); Vaubourdolle, M. [Laboratoire de Biochimie A, Hopital Saint Antoine (France); Attal, P.; Patureau, R.; Tabsoba, Th. [Service Central de Medecine Nucleaire, Hopital Saint Antoine (France); Mensch, B. [Laboratoire de Biophysique, CHU Saint Antoine (France); Askienazy, Pr.S. [Service Central de Medecine Nucleaire, Hopital Saint Antoine (France)

    1997-12-31

    The importance of clearance measurements in evaluating renal or hepatic function needs not further demonstration. The isotopic methods were proved in renal exploration but their utilization in exploration of hepato-biliary function needs to be evaluated. In this work we have compared the measurement of plasma clearance by Mebrofenine, a radiopharmaceutical of specifically hepatic elimination, with the reference method, the clearance of indocyanine green. On the basis of scintigraphic data from 23 patients who benefited by a hepato-biliary exploration by Mebrofenine-{sup 99m}Tc for pre-surgery account and surveillance, we have determined the plasma clearance of this radiopharmaceutical. The method makes use of the curve of normalized cardiac activity by a blood sampling, taken 60 minutes after injection. The measurement of the clearance of indocyanine green was effected within a delay inferior to 1 month from the moment of biliary scintigraphy. The average clearance of Mebrofenine-{sup 99m}Tc is of 553 ml/min for 1.73 m{sup 2} of corporal surface (typical interval is 374 ml/min), the value of indocyanine green is of 450 ml/min (typical interval is 229 ml/min). The two measurements of clearance are correlated (p = 0.05). In conclusion, the measurement of clearance by Mebrofenine-{sup 99m} appears to be adapted to the evaluation of hepato-biliary function

  12. Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats

    International Nuclear Information System (INIS)

    This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1 ml of 99mTc-DMSA IV (0.48 MBq) was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g) determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (p<0.05) was done. There was a significant reduction in %ATI/g in kidney and blood (p<0.05) of splenectomized animals, a significant increase (p<0.05) of urea (88.8 ± 18.6 mg/dL) and creatinine (0.56 ± 0.08 mg/dL), compared to the controls (51.5±1.6, 0.37±0.02 mg/dL, respectively), as well as increase in platelets and leucocytes, and hematocrit reduction. The analysis of the results indicates that in rats, splenectomy seems to alter the renal function and the uptake of 99mTc-DMSA. (author)

  13. Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats

    Energy Technology Data Exchange (ETDEWEB)

    Acucena, Maria Kadja Meneses Torres; Pereira, Kercia Regina Santos Gomes; Villarim Neto, Arthur; Rego, Amalia Cinthia Meneses [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Centro de Ciencias da Saude; Bernardo-Filho, Mario [Universidade do Estado do Rio de Janeiro (UERJ), RJ (Brazil). Inst. de Biologia Roberto Alcantara Gomes. Dept. de Biofisica e Biometria; Azevedo, Italo Medeiros; Araujo Filho, Irami; Medeiros, Aldo Cunha [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Dept. de Cirurgia]. E-mail: aldo@ufrnet.br

    2008-12-15

    This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1 ml of 99mTc-DMSA IV (0.48 MBq) was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g) determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (p<0.05) was done. There was a significant reduction in %ATI/g in kidney and blood (p<0.05) of splenectomized animals, a significant increase (p<0.05) of urea (88.8 {+-} 18.6 mg/dL) and creatinine (0.56 {+-} 0.08 mg/dL), compared to the controls (51.5{+-}1.6, 0.37{+-}0.02 mg/dL, respectively), as well as increase in platelets and leucocytes, and hematocrit reduction. The analysis of the results indicates that in rats, splenectomy seems to alter the renal function and the uptake of 99mTc-DMSA. (author)

  14. Assessment of various parameters in the estimation of differential renal function using technetium-99m mercaptoacetyltriglycine

    Energy Technology Data Exchange (ETDEWEB)

    Lythgoe, M.F.; Gordon, I.; Khader, Z.; Smith, T.; Anderson, P.J. [Department of Radiology, Great Ormond Street Hospital for Children NHS Trust, London (United Kingdom)

    1999-02-01

    Differential renal function (DRF) is an important parameter that should be assessed from virtually every dynamic renogram. With the introduction of technetium-99m mercaptoacetyltriglycine ({sup 99m}Tc-MAG3), a tracer with a high renal extraction, the estimation of DRF might hopefully become accurate and reproducible both between observers in the same institution and also between institutions. The aim of this study was to assess the effect of different parameters on the estimation of DRF. To this end we investigated two groups of children: group A, comprising 35 children with a single kidney (27 of whom had poor renal function), and group B, comprising 20 children with two kidneys and normal global function who also had an associated {sup 99m}Tc-dimercaptosuccinic acid scan ({sup 99m}Tc-DMSA). The variables assessed for their effect on the estimation of DRF were: different operators, the choice of renal regions of interest (ROIs), the applied background subtraction, and six different techniques for analysis of the renogram. The six techniques were based on: linear regression of the slopes in the Rutland-Patlak plot, matrix deconvolution, differential method, integral method, linear regression of the slope of the renograms, and the area under the curve of the renogram. The estimation of DRF was less dependent upon both observer and method in patients with two normally functioning kidneys than in patients with a single kidney. The inter-observer comparison among children in either group was not dependent on either ROI or background subtraction. However, in patients with poor renal function the method of choice for the estimation of DRF was dependent on background subtraction, though not ROI. In children with two kidneys and normal renal function, the estimation of DRF from the 24 techniques gave similar results. Methods that produced DRF values closest to expected results, from either group of children, were the Rutland-Patlak plot and matrix deconvolution methods

  15. The role of pharmacological stress Tc-99m sestamibi myocardial perfusion imaging in an Australian population

    International Nuclear Information System (INIS)

    This observational study was performed in order to assess the exercise-related incremental diagnostic accuracy of Tc-99m sestamibi myocardial perfusion imaging (MPI) in a hospital-based Australian population, and to assess the relative roles of exercise and pharmacological stress in myocardial perfusion imaging (MPI). Two hundred and eight adult patients who had both Tc-99m sestamibi myocardial perfusion imaging and coronary angiography within a median time of 16 weeks were studied. The diagnostic end-point was coronary artery lesions of ≥50% and >70% stenosis detected on angiography. Using receiver operating characteristic curve analysis, the overall diagnostic accuracy was calculated, as well as the comparative accuracies in patients who undertook various levels of exercise stress testing (n=130) and those who received pharmacological (dipyridamole) stress testing (n=78). The overall respective diagnostic accuracy of Tc-99m sestamibi MPI for the diagnosis of coronary artery disease (>70% stenosis) was 81% and 76% when using the diagnostic criterion of ≥50% stenosis. On direct comparison of perfusion defects with angiographic stenoses, the respective sensitivity and specificity for the detection of >70% stenosis in each coronary artery territory was 73% and 79%. Pharmacological MPI showed a significantly greater sensitivity for the detection of localised stenoses compared to the overall group who had exercise MPI performed. Consequently, exercise stress MPI showed significantly more false negative lesions compared to dipyridamole stress imaging (p<0.003). However, a large proportion of patients were unable to perform to adequate exercise levels in this patient sample. We conclude that Tc-99m sestamibi myocardial perfusion imaging is an accurate non-invasive test for the diagnosis of coronary artery disease. Where any doubt exists as to the patient's ability to achieve exercise levels at or above 85% of the predicted value for age and gender, pharmacological

  16. Disintegration rate of Tc -99m and In -111 radioactive solutions in coincidence systems

    International Nuclear Information System (INIS)

    The 111In and 99mTc standardization in a 4πβ-γ coincidence system is described. The 111In was produced by the reaction of 111Cd (p, n) 111In in the cyclotron. The 111In decays with a half life of 2.8 days by electron capture process, populating the excited levels of 111Cd, emitting two main gamma rays with energies of 171 keV and 245 keV. The 99mTc decay with a half life of 6.007 h for isomeric transition, from the radioactive decay of 99Mo. 111In standardization was carried out in a 4πβ-γ system, consisted of a gas flow proportional counter with 4π geometry coupled to a pair of NaI(Tl) scintillation counter with conventional electronics. The gamma window was set comprising the (171 keV + 245 keV) total absorption energy peaks. The choice of the window was based on the analysis of the extrapolation curves prediction, obtained by Monte Carlo simulation. The 99mTc standardization has been accomplished by the 4πβ-γ coincidence method using a thin window proportional counter in a 4π geometry coupled to a single NaI(Tl) scintillation counter. The beta efficiency was varied by electronic discrimination using a software coincidence counting system (SCS). Two windows were selected for the gamma channel: one at 140 keV gamma ray and the other at 20 keV X ray total absorption peaks. The result of the experimental activity of 111In two solutions agree with the results obtained by Monte Carlo simulation. The experimental activities of 99mTc for the two gamma windows are in agreement within the experimental uncertainty, indicating that the adopted methodology is adequate. (author)

  17. Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA in rats

    Directory of Open Access Journals (Sweden)

    Maria Kadja Meneses Torres Açucena

    2008-12-01

    Full Text Available This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6 and control (n = 6. After splenectomy (15 days, the administration of 0.1ml of 99mTc-DMSA IV (0.48 MBq was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (pEstudo com objetivo de avaliar se a esplenectomia altera a biodistribuição do 99mTc-DMSA e alguns parâmetros bioquímicos e hematológicos em ratos Wistar. Os animais forma divididos em 2 grupos: esplenectomizados (n=6 e controle(n=6. Após 15 dias, administração de 0,1 ml de 99mTc-DMSA via plexo orbital (0,48 MBq foi realizada. Rim, coração, pulmão, tireóide, estômago, bexiga e fêmur e amostras de sangue foram separadas. Após pesagem e contagem da radioatividade foi determinado o percentual de radioatividade/g (% ATI/g. Dosadas uréia e creatinina sérica, hematócrito, plaquetas e leucócitos. Estatística pelo teste t, significância 0,05 foi realizada. Foi observada redução significante no %ATI/g no rim e sangue (p<0,05 dos animais esplenectomizados, aumento significante (p<0.05 da uréia (88,8±18,6 mg/dL e creatinina (0,56±0,08, comparado aos controles (51,5±1,6; 0,37±0,02mg/dL, respectivamente assim como aumento de leucócitos e plaquetas e redução de hematócrito. Conclui-se que em ratos, a esplenectomia alterou a captação de 99mTc-DMSA pelo rim, e a função renal.

  18. Diagnostische Bedeutung von pentavalenter 99m Tc-Dimercaptobern-steinsaure ([V]-DMSA) bei Kopf-Hals-Tumoren

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The purpose of this investigation was to assess the biodistribution of 99m Tc-[V]-DMSA in human being,and its diagnostic value in patients with head and neck tumours.20 patients with histologically confirmed head and neck tumours were examined with planar as well as SPECT scintigraphy. Whole body scintigraphy in different time after injection of 99m Tc-[V]-DM-SA was performed to assess the major sequential organ biodistribution. Our results showed that the blood clearance of 99m Tc-[V]-DMSA was bi-exponential. All organs except kidneys showed a relatively rapid elimination of 99m Tc-[V]-DMSA. The kidneys showed a increasing accumulation in the first 2h, which is probably due to the tubular mabsorption of 99m Tc-[V]-DMSA. In 15 of 20 patients, 19 lesions could be proven by means of planar scintigraphy (corresponding sensitivity of 75%). 29 lesions in 18 patients could however be detected by the application of SPECT (corresponding sensitivity of 90%). Except primary tumours and local lymphadenmetastases in 5 patients distant metastases (3 thorax wall,I liver and I ingvinal/paravesicle) were found. Altogether a sensitivity of 76.9% and a specificity of 71.4% were calculated for the detection of primary tumour. The sensitivity and specificity for the exploration of lymphadenmetastases were 75% and 100%. In conclusion, this study shows that 99m Tc-[V]-DMSA, particularly with SPECT imaging, is useful in localising the primary tumours and lymphadenmetastases as well as distant metastases of head and neck tumours. The possible therapeutic application of 188/186Re-[V]-DMSA is also evaluated.

  19. Phase III multi-center clinical study on sup 99m Tc-GSA, a new agent for functional imaging of the liver

    Energy Technology Data Exchange (ETDEWEB)

    Torizuka, Kanji (Fukui Medical School, Matsuoka (Japan)); Ha-Kawa, Sang Kil; Kudo, Masatoshi (and others)

    1992-02-01

    A multi-center clinical study was performed in patinets with hepatic disorders to evaluate the clinical usefulness of {sup 99m}Tc-DTPA-galactosyl serum albumin ({sup 99m}Tc-GSA), a new radiopharmaceutical which binds to asialoglycoprotein receptors on hepatocytes. The blood clearance and hepatic accumulation were evaluated on the basis of the dynamic data and serial hepatic images obtained for 20 min after {sup 99m}Tc-GSA injection. The blood clearance and hepatic accumulation indices of {sup 99m}Tc-GSA demonstrated the followings. In acute liver diseases, these indices reflected the clinical features of the disease and correlated with the laboratory test indices for the blood coagulation system. In chronic liver diseases, these indices changed in direct proportion to the progression of the hepatic disorder and correlated well with the conventional laboratory test results. In obstructive jaundice, these indices aided evaluation of the liver function despite the high serum bilirubin level. The indices reflected the change in the number of hepatocytes before and after hepatectomy. The scintigraphic findings with {sup 99m}Tc-GSA permitted both functional and morphological evaluations of the liver and provide additional information compared with conventional liver scintigraphy. These results suggest that {sup 99m}Tc-GSA scintigraphy may be useful for evaluating both the functional and morphology of the liver from a new viewpoint of receptor-mediated accumulation. (author).

  20. Quantification of ocular inflammation with technetium-99m glucoheptonate

    International Nuclear Information System (INIS)

    Histological and morphometric evaluation of ocular inflammation is difficult, particularly when there is extensive ocular involvement with abscess formation and necrosis. A quantitative imaging procedure applicable to humans would be important clinically. To establish such a procedure, turpentine-induced ocular inflammation was obtained by subconjunctival injection in the right eye of 55 rabbits. The left eye was used as control and injected with a volume of saline equal to the volume of turpentine in the right eye. Volumes of turpentine or saline were 0.02, 0.04, 0.06, 0.2 and 0.6 ml, and the rabbits were divided into groups 1-5, according to these volumes. Imaging was performed 48 h after turpentine injection and 6 h after intravenous injection of 10 mCi of technetium 99m glucoheptonate (99mTc-GH). An inflammatory reaction index (IRI), defined as the ratio of counts of the right eye divided by counts of the left eye, was used. IRIs were proportional to the degree of inflammation and allowed the distinction of 3 subgroups: One represented by group 4, one by group 5 and one by groups 1, 2 and 3. This method of quantification of ocular inflammatory processes using 99mTc-GH is original, rapid, non-invasive, reproducible and safe, although unable to differentiate inflammatory processes caused by doses of turpentine which are very small and close to each other. It is conceivable that its application to humans will bring new insight into the ocular inflammatory process and response to therapy. (orig.)

  1. Sup(99m)Tc-DTPA radio aerosol ventilation study

    International Nuclear Information System (INIS)

    Papers on deposition pattern analyses of radioaerosol lung scans are few and not easy to interpret as there is a general lack of technical standardization and the number of patients studied is not large. Moreover these reports have been generally obtained with non-hydrosoluble radioaerosols. In the present study 43 patients underwent conventional pulmonary function testing and lung scanning using small droplet (equal or less than 2 microns) polydisperse sup(99m)Tc-DTPA radioaerosol produced with the ''Settling Bag System'' Medi 400 (Sorin). The scans were analysed by two methods: 1) a semiquantitative method proposed by Taplin (SQT); 2) an original simpler semiquantitative method (SQM). Correlations of SQT and SQM with FEV1 and MEF75 resulted highly significant (p<0,001). SQM proved to be superior so SQT as far as reproducibility is concerned. The following conclusions can be drawn: 1) small particle sup(99m)Tc-DTPA aerosol can be easily and cheaply produced by a commercial device; 2) such a radioaerosol is well suited for the evaluation of small and large airways patency, as reflected by MEF75 and FEV1 respectively, with a diagnostic yield comparable to non-hydrosoluble radioaerosols; 3) the original semiquantitative description of the deposition pattern proposed and validated in this study is quite easy to implement and it yields a high correlation with pulmonary function tests; moreover such an approach does not require digital data processing; 4) the sensitivity of sup(99m)Tc-DTPA for the diagnosis of bronchial obstruction is very high and superior to routine pulmonary function tests; in addition the ventilation scan allows the topographical localization of the obstruction themselves

  2. Standardization of (99m)Tc by means of a software coincidence system.

    Science.gov (United States)

    Brito, A B; Koskinas, M F; Litvak, F; Toledo, F; Dias, M S

    2012-09-01

    The procedure followed by the Nuclear Metrology Laboratory, at IPEN, for the primary standardization of (99m)Tc is described. The primary standardization has been accomplished by the coincidence method. The beta channel efficiency was varied by electronic discrimination using a software coincidence counting system. Two windows were selected for the gamma channel: one at 140 keV gamma-ray and the other at 20 keV X-ray total absorption peaks. The experimental extrapolation curves were compared with Monte Carlo simulations by means of code ESQUEMA.

  3. Normal fasting and postprandial diisopropyl-IDA Tc 99m hepatobiliary stud

    International Nuclear Information System (INIS)

    Diisopropyl-IDA TC 99m imaging studies were performed in 11 normal subjects in both the fasting and postprandial states. In 5- to 60-minute analog images obtained in both fasting and postprandial studies, the cardiac blood pool was almost never seen, renal pelvic radioactivity was commonly seen, the extrahepatic biliary tract was always seen, and the left hepatic duct was always more prominent than the right hepatic duct. The billiary tract was visualized by ten minutes in nine of 11 fasting studies and 10 of 11 postprandial studies. The gallbladder was visualized in all eleven fasting studies, but in only four postprandial studies (p<0.05). The zero- to sixty-minute digital data indicated a greater hepatocyte clearance, an earlier time of peak parenchymal radioactivity, and a faster parenchymal washout in the postprandial studies compared with fasting studies (p<0.05). Approximately nine percent of the injected dose was recovered in the urine during the first three hours in fasting and postprandial studies. The normal diisopropyl-IDA Tc 99m study in the fasting and postprandial states is defined; significant differences exist between the two states

  4. Changes in renal uptake of Tc-99m methylene diphosphonate (MDP) in stone-forming rats

    International Nuclear Information System (INIS)

    A pyridoxine (vitamin B6)-deficient diet in rats was used as a model of early renal lithiasis to find out if stone-formers could be identified from control animals by differences in the biodistribution of Tc-99m MDP. The mean renal uptake of this agent at 3 hours was about 70% higher in test animals than in controls, but there was considerable overlap between the upper limits of the normal range and lower values in stone-formers. If these results were valid for humans, the metabolic abnormality in males with early stone-forming disease could not be identified with certainty by in vivo measurements of Tc-99m MDP renal uptake alone. However, the skeletal uptake of MDP in the stone-forming animals was depressed by 28 to 35%, compared with control rats. Consequently, the renal to skeletal MDP concentration ratio was invariably elevated in stone-formers beyond the 95 percentile normal range. Unexpectedly, 76% of the pyridoxine-deficient animals had a higher accumulation of MDP in the myocardium than the upper limit of the normal range. The pyridoxine-deficient diet induced no remarkable early changes in the biodistribution or renal clearance of I-131 Hippuran

  5. Ventilation imaging with 99m technetium labeled aerosols

    International Nuclear Information System (INIS)

    The major clinical use of ventilation perfusion scintigraphy is for the diagnosis of pulmonary embolism (PE). Accurate diagnosis of PE is essential since effective treatment if available. The scintigraphic characteristic of PE are segmental perfusion defects in lung that is normally ventilated. The inherent shortcoming of perfusion scintigraphy is its lack of specificity. Combining a ventilation study with perfusion imaging improves the diagnostic specificity of lungs scintigraphy. This article describes clinical use of simple same day ventilation and perfusion imaging technique. The technique is based on a low dose ventilation procedure using 99m Tc labelled aerosol immediately followed by a standard dose perfusion procedure. (author)

  6. Technetium 99m pertechnetate thyroid scintigraphy: Congenital hypothyroid screening

    International Nuclear Information System (INIS)

    Technetium 99m pertechnetate thyroid scans were performed on 57 infants referred for evaluation of suspected congenital hypothyroidism. Thyroid anatomy may be characterized by four general types, based on the scintigraphic findings: (1) normal size and location. (2) ectopic location. (3) no detectable thyroid activity. (4) normal location with increased size or uptake. There are diverse etiologies of congential hypothyroidism. Correlation of thyroid scintigraphy with blood T4 and TSH levels allows specific etiological diagnosis in the majority of cases of congential hypothyroidism. (orig.)

  7. Technetium 99m pertechnetate scans in congenital hypothyroidism

    International Nuclear Information System (INIS)

    Goiters are rarely palpable in infants with congenital hypothyroidism except in the case of maternal ingestion of iodide. The presence or absence of glandular tissue is, however, important for genetic and prognostic counseling and for acceleration of diagnosis in other affected siblings. The detection of thyroid tissue by /sup 99m/Tc pertechnetate scans in a significant number of our patients heretofore considered athyreotic establishes that physical findings and traditional laboratory data are not adequate to determine whether or not thyroid tissue is present

  8. Assessment of renal function in mice with unilateral ureteral obstruction using 99mTc-MAG3 dynamic scintigraphy

    Directory of Open Access Journals (Sweden)

    Tantawy Mohammed N

    2012-12-01

    Full Text Available Abstract Background Renal scintigraphy using 99mTc-mercaptoacetyltriglycine (99mTc-MAG3 is widely used for the assessment of renal function in humans. However, the application of this method to animal models of renal disease is currently limited, especially in rodents. Here, we have applied 99mTc-MAG3 renal scintigraphy to a mouse model of unilateral ureteral obstruction (UUO and evaluated its utility in studying obstructive renal disease. Methods UUO mice were generated by complete ligation of the left ureter. Sham-operated mice were used as a control. Renal function was investigated on days 0, 1, 3, and 6 post-surgery using dynamic planar imaging of 99mTc-MAG3 activity following retro-orbital injection. Time-activity curves (TACs were produced for individual kidneys and renal function was assessed by 1 the slope of initial 99mTc-MAG3 uptake (SIU, which is related to renal perfusion; 2 peak activity; and 3 the time-to-peak (TTP. The parameters of tubular excretion were not evaluated in this study as 99mTc-MAG3 is not excreted from UUO kidneys. Results Compared to sham-operated mice, SIU was remarkably (>60% reduced in UUO kidneys at day 1 post surgery and the TACs plateaued, indicating that 99mTc-MAG3 is not excreted in these kidneys. The plateau activity in UUO kidneys was relatively low (~40% of sham kidney’s peak activity as early as day1 post surgery, demonstrating that uptake of 99mTc-MAG3 is rapidly reduced in UUO kidneys. The time to plateau in UUO kidneys exceeded 200 sec, suggesting that 99mTc-MAG3 is slowly up-taken in these kidneys. These changes advanced as the disease progressed. SIU, peak activity and TTPs were minimally changed in contra-lateral kidneys during the study period. Conclusions Our data demonstrate that renal uptake of 99mTc-MAG3 is remarkably and rapidly reduced in UUO kidneys, while the changes are minimal in contra-lateral kidneys. The parametric analysis of TACs suggested that renal perfusion as well as tubular

  9. Biodistribution of {sup 99m}Tc-lactosylated serum albumin in mice with diethylnitrosamine or thiacetamide induced liver injury

    Energy Technology Data Exchange (ETDEWEB)

    Whang, Jae Seok; Ahn, Byeong Cheol; Sung, Young Ok; Seo, Ji Hyoung; Bae, Jin Ho; Jeong, Shin Young; Yoo, Jung Soo; Lee, Jae Tae; Lee, Kyu Bo [Kyungpook National University Medical School, Daegu (Korea, Republic of); Jeong, Jae Min [Seoul National University College of Medicine, Seoul (Korea, Republic of)

    2005-02-15

    Tc-99m labeled diethylenetriaminepentaacetic acid (DTPA)-coupled galactosylated human serum albumin (GSA) is a currently used imaging agent for asialoglycoprotein receptor (ASGPR) of the liver, but it has several shortcomings. Recently a new ASGPR imaging agent, {sup 99m}Tc-lactosylated human serum albumin (LSA), with simple labeling procedure, high labeling efficiency, high stability was developed. In order to assess the feasibility of the {sup 99m}Tc-LSA as a ASGPR imaging radiopharmaceuticals, we performed biodistribution study of the tracer in liver injured mice model and the results were compared with histologic data. To induce hepatic damage in ICR mice, diethylnitrosamine (DEN) (60 mg/kg/week X 5 time, low dose or 180 mg/kg/week X 2 time, high dose) and thioacetamide (TAA) (50 mg/kg X 1 time) were administrated intraperitoneally. Degree of liver damage was evaluated by tissue hematoxilin-eosin stain, and expression of asialoglycoprotein receptor (ASGPR) was assessed by immunohistochemistry using ASGPR antibody. {sup 99m}Tc-LSA was intravenously administrated via tail vein in DEN or TAA treated mice, and biodistribution study of the tracer was also performed. DEN treated mice showed ballooning of hepatocyte and inflammatory cell infiltration in low dose group and severe hepatocyte necrosis in high dose group, and low dose group showed higher ASGPR staining than control mice in immunohistochemical staining. TAA treated mice showed severe hepatic necrosis. {sup 99m}Tc-LSA biodistribution study showed that mice with hepatic necrosis induced by high dose. DEN or TAA revealed higher blood activity and lower liver activity than control mice, due to slow clearance of the tracer by the liver. The degree of liver uptake was inversely correlated with the degree of histologic liver damage. But low dose DEN treated mice with mild hepatic injury showed normal blood clearance and hepatic activity, partly due to overexpression of ASGPR in mice with mild degree hepatic

  10. Technetium-99m-human polyclonal IgG radiolabeled via the hydrazino nicotinamide derivative for imaging focal sites of infection in rats

    International Nuclear Information System (INIS)

    The biologic behavior of human polyclonal immunoglobulin (IgG) radiolabeled with technetium-99m (99mTc) by a novel method, via a nicotinyl hydrazine derivative, was evaluated in rats. Technetium-99m- and indium-111-IgG were co-administered to normal rats and biodistribution was determined at 2, 6, and 16 hr. The inflammation imaging properties of the two reagents were compared in rats with deep-thigh infection due to Escherichia coli. Blood clearance of both antibody preparations was well described by a bi-exponential function: (99mTc-IgG: t1/2 = 3.82 +/- 0.89 and 57.52 +/- 1.70 hr. 111In-IgG: 3.93 +/- 0.117 and 40.71 +/- 1.26 hr). Biodistributions in the solid organs were similar, however, small but statistically significant differences were detected: 99mTc-IgG greater than 111In-IgG in lung, liver, and spleen; 99mTc-IgG less than 111In-IgG in kidney and skeletal muscle (p less than 0.01). At all three imaging times, target-to-background ratio and percent residual activity for the two compounds were remarkably similar. These studies establish that human polyclonal IgG labeled with 99mTc via a nicotinyl hydrazine modified intermediate is equivalent to 111In-IgG for imaging focal sites of infection in experimental animals

  11. Technetium-99m NGA functional hepatic imaging: preliminary clinical experience

    International Nuclear Information System (INIS)

    Technetium-99m galactosyl-neoglycoalbumin ( [Tc]NGA) is a radiolabeled ligand to hepatic binding protein, a receptor which resides at the plasma membrane of hepatocytes. This receptor-binding radiopharmaceutical and its kinetic model provide a noninvasive method for the assessment of liver function. Eighteen patients were studied: seven with hepatoma, eight with liver metastases, four with cirrhosis, and one patient with acute fulminant non-A, non-B hepatitis. Technetium-99m NGA liver imaging provided anatomic information of diagnostic quality comparable to that obtained with other routine imaging modalities, including computed tomography, angiography, ultrasound, and [Tc]sulfur colloid scintigraphy. Kinetic modeling of dynamic [Tc]NGA data produced estimates of standardized hepatic blood flow, Q (hepatic blood flow divided by total blood volume), and hepatic binding protein concentration, [HBP]. Significant rank correlation was obtained between [HBP] estimates and CTC scores. This correlation supports the hypothesis that [HBP] is a measure of functional hepatocyte mass. The combination of decreased Q and markedly reduced [HBP] may have prognostic significance; all three patients with this combination died of hepatic failure within 6 wk of imaging

  12. Using radioisotopes compounds (99mTc-infecton and 99mTcHIG) in evaluating and examination of pulmonary tuberculosis patients

    International Nuclear Information System (INIS)

    This study examined 40 patients: 34 have pulmonary tuberculosis, 3 have pneumonitis, 2 have lung cancer, and one has bilharziasis. All patients have been imaged using radioisotopes compounds (99mTc-infecton and 99mTcHIG) and after 1 hour and 4 hours of patients injection.Using 99mTc-infecton gave positive results in 30 pulmonary tuberculosis patients out of 34, the 3 pneumonitis patients, while using 99mTcHIG gave positive results in 27 pulmonary tuberculosis patients including the 3 pneumonitis patients. Both 99mTc-infecton and 99mTcHIG gave negative results with the lung cancer patients and unreal positive results with the bilharziasis patient. It has been found that the sensitivity and accuracy in the examinations using 99mTc-infecton were 88%, 93%, and 85.7% respectively in comparison with using 99mTcHIG where the values were 70.6%, 91%, and 68.5% respectively. Continuing examination and monitoring of 18 tuberculosis patients for 2-18 months with anti-pulmonary tuberculosis treatment, showed complete response of 12 patients using 99mTc-infecton, and 8 by using 99mTcHIG. As a result, 99mTc-infecton can be used for examining pulmonary tuberculosis patients

  13. Study on 99mTc-MAG3 and 99mTc-DMSA renal accumulation using in vitro cellular model.

    Science.gov (United States)

    Nový, Zbynĕk; Mandíková, Jana; Trejtnar, Frantisek

    2011-02-01

    Mercaptoacetyltriglycine (MAG3) and dimercaptosuccinic acid (DMSA) labelled with technetium-99m belongs to standard renal radiodiagnostics. However, the renal transport mechanisms responsible for their high renal uptake have not been fully explained. In addition, no in vitro experimental study comparing the renal uptake of these radiopharmaceuticals at the cellular level has not been performed. The investigation compared the 99mTc-MAG3 and 99mTc-DMSA renal uptake using primary rat renal cells and evaluated contribution of active and passive transport processes to the renal accumulation. The renal cells were isolated from the rat kidneys by means of the two-phase collagenase perfusion method. The used experimental model showed to be useful tool for such type of investigation. The results documented significant quantitative and qualitative differences in the accumulation of 99mTc-DMSA and 99mTc-MAG3 in the rat isolated cells. The found experimental data indicated several times higher uptake of 99mTc-MAG3 than that found in 99mTc-DMSA. 99mTc-MAG3 cellular uptake was substantially decreased when active, energy-dependent processes were inhibited. However, 99mTc-DMSA accumulation in the renal cells demonstrated only a minor dependency on energy. These findings demonstrate a very different character of the membrane transport determining 99mTc-DMSA and 99mTc-MAG3 renal accumulation.

  14. Pulmonary clearance of 99mTc-DTPA: role of the pulmonary surfactant system.

    OpenAIRE

    Bos, Jelle

    1992-01-01

    textabstractIt is established that in a wide variety of acute and chronic lung diseases the integrity of the alveolar-capillary membrane is altered. Increased permeability of the barrier is thought to cause increased filtration of fluids and proteins into the alveoli and increased absorption of inhaled substances into the blood stream. This can eventually lead to impaired gas exchange and, therefore, deterioration of tissue oxygenation. Attempts to elucidate the nature of changes occurring in...

  15. Preparation of chromatographic and solid-solvent extraction 99mTc generators using gel-type targets

    International Nuclear Information System (INIS)

    We have studied two types of targets zirconium-molybdate (ZrMo) and titanium-molybdate (TiMo) prepared by precipitating reaction between ammonium-molybdate and zirconium-chloride or titanium-chloride solutions, respectively. Other types of targets were also prepared by co-precipitating ZrMo or TiMo with hydrous manganese-dioxide, hydrous silica, and hydrous titanium-dioxide or by impregnated ZrMo or TiMo with Iodate anions. The results on extraction of Tc-99m from neutron irradiated TiMo solid phase using solvents such as MEK, aceton, ethylic ether, chloroform, etc showed that separation yield (SY) of Tc-99m in case of aceton extraction was from 70% to 80% and in other cases non higher than 40%. The Tc-99m elution curves and column kinetic in case of aceton extraction (after evaporation of aceton and recovery of Tc-99m in 0,9% NaCl solution) was superior than in case chromatographic generator using saline eluant. As result obtained, two types of generators were successfully prepared and put into use: Chromatographic generator using titanium-molybdate target as packing material and saline as eluant. Solid-solvent extraction 99mTc generator using titanium-molybdate target (as solid phase) and aceton as extracting solvent. (author)

  16. Preparation of chromatographic and solid-solvent extraction {sup 99m}Tc generators using gel-type targets

    Energy Technology Data Exchange (ETDEWEB)

    Le Van So [Radioisotope Department, Nuclear Research Institute, Dalat (Viet Nam)

    2000-10-01

    We have studied two types of targets zirconium-molybdate (ZrMo) and titanium-molybdate (TiMo) prepared by precipitating reaction between ammonium-molybdate and zirconium-chloride or titanium-chloride solutions, respectively. Other types of targets were also prepared by co-precipitating ZrMo or TiMo with hydrous manganese-dioxide, hydrous silica, and hydrous titanium-dioxide or by impregnated ZrMo or TiMo with Iodate anions. The results on extraction of Tc-99m from neutron irradiated TiMo solid phase using solvents such as MEK, aceton, ethylic ether, chloroform, etc showed that separation yield (SY) of Tc-99m in case of aceton extraction was from 70% to 80% and in other cases non higher than 40%. The Tc-99m elution curves and column kinetic in case of aceton extraction (after evaporation of aceton and recovery of Tc-99m in 0,9% NaCl solution) was superior than in case chromatographic generator using saline eluant. As result obtained, two types of generators were successfully prepared and put into use: Chromatographic generator using titanium-molybdate target as packing material and saline as eluant. Solid-solvent extraction {sup 99m}Tc generator using titanium-molybdate target (as solid phase) and aceton as extracting solvent. (author)

  17. Synthesis and characterisation of technetium-99m labelled ciprofloxacin (Infecton)

    International Nuclear Information System (INIS)

    Full text: Infecton is Tc-99m labelled Ciprofloxacin, which is a synthetic carboxyquinolone derivative with broad-spectrum antimicrobial activity. It is a new class of radiopharmaceutical designed for imaging live bacterial infection. Conventional imaging agents such as Tc-99m or In-111 labelled leucocytes are either time-consuming or hazardous due to blood handling. These can be obviated by the use of Infecton which we synthesised by modifying the procedure described by Britton and co-workers (Lancet 1996;..347: 233-235). Bioassay and animal studies have been performed with a view to its use in infection imaging in patients. The first task was to prepare pure ciprofloxacin from its commercially available lactate salt. This was achieved by isoelectric precipitation at pH 8.6 by adding sodium hydroxide to the formulation. The resultant precipitate was washed with 200 mL of water for injection and filtered through a cintered-glass filtering unit. The precipitate was free of lactate and sodium as analysed by Biochem analyser. The UV spectrophotometric analysis showed an absorption peak at 276.3 nm which is close to the theoretical value of 277 nm, thus confirming the purity of the compound. Infecton was synthesised by adding 2 mg of pure ciprofloxacin, 0.4 mg of formimidine sulfinic acid ( non-stannous reducing agent) and 1.0 GBq Tc-99m pertechnetate in a final volume of 1.0 mL saline into a sterile N2-filled Amersham vial and boiling the mixture at 100 deg C for 10 min. The purity of the product was 40-45%. It was passed through a DEAE Sephadex A-25 column and eluted with phosphate buffered saline (0.01M, pH 6.9) with a purity of >96%. The preparation was characterised by bioassay (n=3) by adding Infecton to four different broths of bacterial strains viz Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and MRSA with resultant binding of 50.4, 45.6, 48.5 and 50.3% respectively. The binding was less than 1.0% when either ciprofloxacin or

  18. 99mTc: Labeling Chemistry and Labeled Compounds

    Science.gov (United States)

    Alberto, R.; Abram, U.

    This chapter reviews the radiopharmaceutical chemistry of technetium related to the synthesis of perfusion agents and to the labeling of receptor-binding biomolecules. To understand the limitations of technetium chemistry imposed by future application of the complexes in nuclear medicine, an introductory section analyzes the compulsory requirements to be considered when facing the incentive of introducing a novel radiopharmaceutical into the market. Requirements from chemistry, routine application, and market are discussed. In a subsequent section, commercially available 99mTc-based radiopharmaceuticals are treated. It covers the complexes in use for imaging the most important target organs such as heart, brain, or kidney. The commercially available radiopharmaceuticals fulfill the requirements outlined earlier and are discussed with this background. In a following section, the properties and perspectives of the different generations of radiopharmaceuticals are described in a general way, covering characteristics for perfusion agents and for receptor-specific molecules. Technetium chemistry for the synthesis of perfusion agents and the different labeling approaches for target-specific biomolecules are summarized. The review comprises a general introduction to the common approaches currently in use, employing the N x S4-x , [3+1] and 2-hydrazino-nicotinicacid (HYNIC) method as well as more recent strategies such as the carbonyl and the TcN approach. Direct labeling without the need of a bifunctional chelator is briefly reviewed as well. More particularly, recent developments in the labeling of concrete targeting molecules, the second generation of radiopharmaceuticals, is then discussed and prominent examples with antibodies/peptides, neuroreceptor targeting small molecules, myocardial imaging agents, vitamins, thymidine, and complexes relevant to multidrug resistance are given. In addition, a new approach toward peptide drug development is described. The section

  19. Cardiac amyloidosis detection with pyrophosphate-99mTc scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Souza, D.S.F.; Ichiki, W.A.; Coura Filho, G.B.; Izaki, M.; Giorgi, M.C.P.; Soares Junior, J; Meneghetti, J.C. [Universidade de Sao Paulo (FM/USP), SP (Brazil). Fac. de Medicina. Instituto do Coracao. Servico de Medicina Nuclear e Imagem Molecular

    2008-07-01

    Full text: Introduction: Amyloidosis is a rare disease, characterized by extracellular deposition of insoluble amyloid fibrils in organs and tissues. It may affect virtually any system, preferably heart, kidneys and liver. The cardiac involvement produces a spectrum of clinical features, usually with progressive dysfunction. Early diagnosis is important for institution of appropriate therapy. Case report: Male patient, 75 years old, with diagnosed congestive heart failure functional class III and Mobitz II second-degree atrial-ventricular block, was hospitalized for implantation of definitive cardiac pacemaker. Patient mentioned history of worsening effort dyspnoea over a one-month period, progressing to minimum effort, orthopnea, paroxysmal nocturnal dyspnoea and paroxysms of dry cough, and swelling of lower limbs. Echocardiography showed diffuse hypertrophy of left ventricle (LV), with systolic dysfunction due to diffuse hypokinesia and hyperrefringent aspect in the septum. It was questioned a cardiac infiltrating process. Cardiac amyloidosis was considered as a diagnostic hypothesis. The patient underwent a pyrophosphate-{sup 99m}Tc scintigraphy, which showed abnormal tracer uptake in the heart projection, with diffuse pattern on the left ventricle walls, compatible with the clinical suspicion cardiac amyloidosis, which was later confirmed by endomyocardial biopsy. Discussion: In this case report, the patient had clinical and other auxiliary examinations, such as electrocardiography and Doppler echocardiography, compatible with cardiac amyloidosis, which led to implementation with pyrophosphate-{sup 99m}Tc scintigraphy and later endomyocardial biopsy. Cardiac amyloidosis occurs in about half the cases of primary amyloidosis (AL) and is rare in secondary amyloidosis (AA). Its clinical presentation is polymorphic and it can be classified into four distinctive types: restrictive cardiomyopathy, systolic dysfunction, postural hypotension and conduction disorders

  20. Synthesis and characterisation of technetium-99m labelled ciprofloxacin (Infecton)

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, V.; Choong, K.K.L.; Olma, T.R.; Mieczkowski, S. [Westmead and the New Childrens Hospital, Westmead, NSW (Australia). Department of Nuclear Medicineand Ultrasound and Centre for infectious Diseases and Microbiology laboratory Services

    1998-06-01

    Full text: Infecton is Tc-99m labelled Ciprofloxacin, which is a synthetic carboxyquinolone derivative with broad-spectrum antimicrobial activity. It is a new class of radiopharmaceutical designed for imaging live bacterial infection. Conventional imaging agents such as Tc-99m or In-111 labelled leucocytes are either time-consuming or hazardous due to blood handling. These can be obviated by the use of Infecton which we synthesised by modifying the procedure described by Britton and co-workers (Lancet 1996;..347: 233-235). Bioassay and animal studies have been performed with a view to its use in infection imaging in patients. The first task was to prepare pure ciprofloxacin from its commercially available lactate salt. This was achieved by isoelectric precipitation at pH 8.6 by adding sodium hydroxide to the formulation. The resultant precipitate was washed with 200 mL of water for injection and filtered through a cintered-glass filtering unit. The precipitate was free of lactate and sodium as analysed by Biochem analyser. The UV spectrophotometric analysis showed an absorption peak at 276.3 nm which is close to the theoretical value of 277 nm, thus confirming the purity of the compound. Infecton was synthesised by adding 2 mg of pure ciprofloxacin, 0.4 mg of formimidine sulfinic acid ( non-stannous reducing agent) and 1.0 GBq Tc-99m pertechnetate in a final volume of 1.0 mL saline into a sterile N{sub 2}-filled Amersham vial and boiling the mixture at 100 deg C for 10 min. The purity of the product was 40-45%. It was passed through a DEAE Sephadex A-25 column and eluted with phosphate buffered saline (0.01M, pH 6.9) with a purity of >96%. The preparation was characterised by bioassay (n=3) by adding Infecton to four different broths of bacterial strains viz Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and MRSA with resultant binding of 50.4, 45.6, 48.5 and 50.3% respectively. The binding was less than 1.0% when either ciprofloxacin or

  1. Different uptake of {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO in the same brains: analysis by statistical parametric mapping

    Energy Technology Data Exchange (ETDEWEB)

    Hyun, I.Y. [Dept. of Nuclear Medicine, Inha University College of Medicine, Incheon (Korea); Lee, J.S.; Lee, D.S. [Dept. of Nuclear Medicine, Seoul National University College of Medicine, Seoul (Korea); Rha, J.H.; Lee, I.K.; Ha, C.K. [Dept. of Neurology, Inha University College of Medicine, Incheon (Korea)

    2001-02-01

    The purpose of this study was to investigate the differences between technetium-99m ethyl cysteinate dimer ({sup 99m}Tc-ECD) and technetium-99m hexamethylpropylene amine oxime ({sup 99m}Tc-HMPAO) uptake in the same brains by means of statistical parametric mapping (SPM) analysis. We examined 20 patients (9 male, 11 female, mean age 62{+-}12 years) using {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO single-photon emission tomography (SPET) and magnetic resonance imaging (MRI) of the brain less than 7 days after onset of stroke. MRI showed no cortical infarctions. Infarctions in the pons (6 patients) and medulla (1), ischaemic periventricular white matter lesions (13) and lacunar infarction (7) were found on MRI. Split-dose and sequential SPET techniques were used for {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO brain SPET, without repositioning of the patient. All of the SPET images were spatially transformed to standard space, smoothed and globally normalized. The differences between the {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO SPET images were statistically analysed using statistical parametric mapping (SPM) 96 software. The difference between two groups was considered significant at a threshold of uncorrected P values less than 0.01. Visual analysis showed no hypoperfused areas on either {sup 99m}Tc-ECD or {sup 99m}Tc-HMPAO SPET images. SPM analysis revealed significantly different uptake of {sup 99m}Tc-ECD and {sup 99m}Tc-HMPAO in the same brains. On the {sup 99m}Tc-ECD SPET images, relatively higher uptake was observed in the frontal, parietal and occipital lobes, in the left superior temporal lobe and in the superior region of the cerebellum. On the {sup 99m}Tc-HMPAO SPET images, relatively higher uptake was observed in the medial temporal lobes, thalami, periventricular white matter and brain stem. These differences in uptake of the two tracers in the same brains on SPM analysis suggest that interpretation of cerebral perfusion is possible using SPET with {sup 99m}Tc-ECD and

  2. Different uptake of 99mTc-ECD and 99mTc-HMPAO in the same brains: analysis by statistical parametric mapping

    International Nuclear Information System (INIS)

    The purpose of this study was to investigate the differences between technetium-99m ethyl cysteinate dimer (99mTc-ECD) and technetium-99m hexamethylpropylene amine oxime (99mTc-HMPAO) uptake in the same brains by means of statistical parametric mapping (SPM) analysis. We examined 20 patients (9 male, 11 female, mean age 62±12 years) using 99mTc-ECD and 99mTc-HMPAO single-photon emission tomography (SPET) and magnetic resonance imaging (MRI) of the brain less than 7 days after onset of stroke. MRI showed no cortical infarctions. Infarctions in the pons (6 patients) and medulla (1), ischaemic periventricular white matter lesions (13) and lacunar infarction (7) were found on MRI. Split-dose and sequential SPET techniques were used for 99mTc-ECD and 99mTc-HMPAO brain SPET, without repositioning of the patient. All of the SPET images were spatially transformed to standard space, smoothed and globally normalized. The differences between the 99mTc-ECD and 99mTc-HMPAO SPET images were statistically analysed using statistical parametric mapping (SPM) 96 software. The difference between two groups was considered significant at a threshold of uncorrected P values less than 0.01. Visual analysis showed no hypoperfused areas on either 99mTc-ECD or 99mTc-HMPAO SPET images. SPM analysis revealed significantly different uptake of 99mTc-ECD and 99mTc-HMPAO in the same brains. On the 99mTc-ECD SPET images, relatively higher uptake was observed in the frontal, parietal and occipital lobes, in the left superior temporal lobe and in the superior region of the cerebellum. On the 99mTc-HMPAO SPET images, relatively higher uptake was observed in the medial temporal lobes, thalami, periventricular white matter and brain stem. These differences in uptake of the two tracers in the same brains on SPM analysis suggest that interpretation of cerebral perfusion is possible using SPET with 99mTc-ECD and 99mTc-HMPAO. (orig.)

  3. Scintimammography with Tc-99m sestamibi in breast cancer

    International Nuclear Information System (INIS)

    Full text: It is already known that breast cancer is the most common malignancy in women. Physical breast examination and mammography are currently the most recognized screening tools for detection of breast cancer. However, breast mammography has some limitations, especially in women with dense breast or in patients who have had surgery, radiotherapy or chemotherapy. The aim of this study was to investigate the role of Tc-99m Sestamibi scintimammography in the diagnosis of breast cancer. Forty-two women (median age= 46 years, range 21-78 years) were investigated. All had a thorough clinical examination, ultrasonography, mammography, determination of the level of tumor markers - CA15.3 and CEA, fine needle aspiration cytology (FNA) and scintimammography before surgery. The final diagnosis was determined by histopathological examination. For scintimammography, a dose of 740 MBq of Tc-99m sestamibi was injected into a foot vein followed by 10ml saline flush. Imaging was performed 10 minutes later in prone position; lateral views of the breasts and axilla were taken (10 minutes per view, matrix size 128x128, high resolution collimator). Anterior views of the breast and axillary areas were also acquired with patients in supine position. Delayed scintigraphy in prone position (lateral view) was done 60 minutes after injection. Focal accumulation of sestamibi in tumor on early and delayed scintigraphy was considered positive. The results of scintimammography were compared with histopathology. The histopathological results revealed malignancy in 33 (78.6%) and benign tumors in 9 (21.4%) cases. Scintimammography showed true positive findings in 31 (73.8%) patients having breast cancer. It was false negative in 2 cases (1 of invasive ductal carcinoma and 1 of invasive lobular carcinoma). All 5 cases with nonpalpable invasive ductal carcinoma and two cases with ductal carcinoma in-situ were positive on scintimammography. True negative findings with no focally increased

  4. Somatostatin analogues labelled with 99mTc

    International Nuclear Information System (INIS)

    Biological and radiochemical studies have been carried out on two labelled somatostatin analogues, the peptide RC-150 and the Tyr3-Octreotide. Both analogues have been labelled with 99mTc using the direct and the indirect method and MAG-3 and HYNIC as chelating agents. By the direct method RC-150 was labelled using sodium ascorbate and dithionite as reducing agents. The radiochemical purity was 70%. By the indirect method, in the case of RC-160 with MAG-3 a radiochemical purity higher than 70% was attained while a purity of 100% was reached in the case of Tyr3-Octreotide with HYNIC. The biological distribution of HYNIC-Tyr3-Octreotide has been studied in rats. (author)

  5. In vivo and in vitro study of the 99mTc-DMSA radiopharmaceutical connection to blood elements

    International Nuclear Information System (INIS)

    Radiopharmaceuticals are widely used in nuclear medicine. The comprehension of their uptake mechanism in target organs, as well as their clearance may depend on the elucidation of their biochemical characteristics, for instance, their binding to blood elements. The reported precipitating studies of blood with radiopharmaceuticals have shown that the results can not be easily compared. Then, we decide evaluate of the binding proteins on the blood elements using trichloroacetic acid (TCA) to determine the radioactivity of the dimercaptosuccinic acid with technetium-99m (99mTc-DMSA) present in precipitating plasma (P) and blood cells (BC). Depending on the TCA concentration we have determined different values in the insoluble fractions of the plasma when the in vivo and in vitro evaluations were carried out. (author)

  6. Study of technetium behaviour in radiopharmaceuticals. Characterization of sup(99m)Tc-pyrophosphate, sup(99m)Tc-dimercaptosuccinate, sup(99m)Tc-diethylenetriaminepentaacetate complexes and sup(99m)Tc-colloidal rhenium sulphide

    International Nuclear Information System (INIS)

    The chemistry of technetium in extremely dilute solution was approached through the study of three complexing agents and a colloid. By the application of high-performance chromatographic techniques to the analysis of (Tc-pyro), (Tc-DTPA), (Tc-DMSA) complexes it was possible to isolate one or more chelates from a single complexing agent. Addition of pertechnetates to a solution of sodium pyrophosphates and stannous chloride at neutral pH leads to the formation of two complexes, both highly osteotropic. By the use of sup(117m)Sn it was shown that tin employed as reducing agent enters into the composition of one of the two complexes, either of which may be obtained preferentially by varying the (Sn)/(pyro) ratio. With technetium at acid pH (2.5) DMSA gives one or more chelates according to the concentration of the reagents present. DTPA with technetium at neutral pH gives a single complex for which a structure is proposed. The addition of calcium, indispensable for DTPA injection, leads to the appearance of a second bimetallic complex in very much smaller proportions than the first. The size distribution of some colloids was studied by ultrafiltration and permeation on gel. The preparation of colloidal rhenium sulphide and the technetium labelling conditions needed to obtain a very fine colloid were developed. The behaviour of technetium in the presence of colloidal rhenium sulphide and tin pyrophosphate was followed by sup(99m)Tc - sup(186)Re and sup(99m)Tc - sup(117m)Sn double-labelling tests. One reduced technetium fraction associates with the hydrolysed tin, the other follows the rhenium sulphide

  7. Effect of cyclophosphamide in blood cells biodistribution of 99 mTc-radiopharmaceuticals; Comparacao do efeito da ciclofosfamida na biodistribuicao sanguinea de radiofarmacos marcados com Tecnecio-99m

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Josefina S.; Braga, Ana C.S.; Gutfilen, Bianca; Bernardo-Filho, Mario [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Dept. de Biofisica e Biometria

    1997-12-01

    When a radiopharmaceutical is administered to a human being, a fraction of is always bound to the blood elements. Several authors have reported that therapeutic drugs can modify the binding of radiopharmaceutical to blood elements, this fact can alter its biodistribution. The aim of this work is to evaluate, in vivo, if the cyclophosphamide (CTX) is capable to alter the binding of {sup 99m} Tc-DTPA, {sup 99m} Tc-MDP, {sup 99m} Tc-GHA, {sup 99m} Tc-PHY and {sup 99m} Tc O{sub 4} to the blood elements.Mice were also treatment with CTX. After the radiopharmaceuticals were also administered to the same animals. Cyclophosphamide altered the percentage of radioactivity: in P for {sup 99m} Tc-PHY and (0,25 to 8 h) for {sup 99m} Tc-MDP; in FI-P (0,25 to 8 h) for {sup 99m} Tc-MDP; in FI-C (2 and 4 h) for {sup 99m} Tc-DTPA and (0,25 to 4 h) for {sup 99m} Tc O{sub 4}. 7 refs., 9 tabs.

  8. Comparison of the Efficiency for Tc-99m Tin-colloid and Tc-99m Phytate in Sentinel Node Detection in Breast Cancer Patients

    International Nuclear Information System (INIS)

    Lymphoscintigraphy and sentinel node biopsy has become a standard method for detection of axillary lymph node metastasis in breast cancer patients, but the standard radiopharmaceutical was not prepared. About detection of axillary lymph node metastasis by lymphoscintigraphy and sentinel node biopsy in breast cancer patient, we compared the results of Tc-99m Tin-colloid and Tc-99m Phytate by subareolar injection. This study included 382 breast cancer patients who were performed operation during 2001-2008. Three hundred forty nine patients was injected 0.8 ml of Tc-99m Tin-colloid (37-185 MBq) by subareolar injection. Thirty three patients was injected 0.8 ml of Tc-99m Phytate (37-185 MBq). Lymphoscintigraphy was performed in supine position and sentinel node localization was performed by hand-held gamma probe in operation. Among 349 patients by Tc-99m Tin-colloid, 312 cases (89.4%) localized the sentinel node by lymphoscintigraphy, 304 cases (87.1%) localized by gamma probe. Among 33 patients by Tc-99m Phytate, 32 cases (97.0%) localized by lymphoscintigraphy, 33 cases (100%) localized by gamma probe. Detection rate by lymphoscintigraphy and gamma probe was superior for Tc-99m Phytate compared to that with Tc-99m Tin-colloid, with a statistically significant difference. (p<0.05, p<0.05) Tc-99m Phytate is a better choice for localization of sentinel node than Tc-99m Tin-colloid in breast cancer patients

  9. Discordant uptake of Tc-99m DTPA-galactosyl human serum albumin and Tc-99m Sn colloid in a patient with severe acute hepatitis.

    Science.gov (United States)

    Miyazaki, C; Matsunaga, T; Kubo, K

    1994-08-01

    A patient with recently diagnosed severe acute hepatitis underwent serial liver scintigraphy with Tc-99m Sn colloid and Tc-99m DTPA-galactosyl human serum albumin. In initial studies, radionuclide distribution on Tc-99m DTPA-galactosyl human serum albumin scintigraphy was completely discrepant to that on Tc-99m Sn colloid scintigraphy. In a follow-up study 1 month later, the distribution of both radionuclides in the liver appeared relatively homogeneous. The uptake of Tc-99m DTPA-galactosyl human serum albumin and Tc-99m Sn colloid reflects the function of hepatocytes and Kupffer cells, respectively. Both kinds of scintigraphic study may be helpful to assess histopathologic change of different hepatic tissue architectures. PMID:7955747

  10. Cyclotron production of NCA 99mTc and 99Mo. An alternative non-reactor supply source of instant 99mTc and 99Mo → 99mTc generators

    International Nuclear Information System (INIS)

    The direct production of no-carrier-added (NCA) 6.02 h 99mTc and of 66 h 99mMo using proton beams on natural Mo targets was investigated. The major objective of this work was to evaluate the potential of utilizing high-intensity proton accelerators as a supply source of 99mTc and 99Mo for use in diagnostic nuclear medicine. The excitation functions for the production of the directly-made 99mTc via the 100Mo(p, 2n)99mTc reaction, and of its parent 99Mo via the 100Mo(p, pn) 99Mo and 100Mo(p, 2p)99mNb(15 s) → 99Mo reactions, were measured in the 68-8 MeV energy range. Single and cumulative yields for 99mTc and 99Mo, and for other Tc, Mo, Zr, Nb and Y radiocontaminants were also determined. The prospects of integrating the use of enriched 100Mo targets with high-intensity, dual beam, H- accelerators was analyzed. The potential of this combined method to replace or complement the current reactor-based supply sources of 99Mo → 99mTc generators, is also discussed. Finally, a brief analysis is made on the potential use of this combined technology to support the anticipated expansion of nuclear medicine in developing nations. (author)

  11. The comparison of two gated SPET protocols: adenosine Tc-99m tetrafosmin and treadmill exercise Tc-99m MIBI

    International Nuclear Information System (INIS)

    The effect of adenosine and exercise on gated SPET left ventricular ejection fraction (LVEF), end diastolic volume (EDV) and end systolic volume (ESV) has not been fully investigated. The aim of the study was to compare functional measurements obtained in one-day adenosine rest and two-day stress-rest protocols in relation to ischaemia. Out of 226 consecutive patients examined with submaximal treadmill stress-rest 700 MBq Tc-99m MIBI, 26 were chosen to match those subjected to adenosine (140 μg/kg/min) enhanced by a low level exercise protocol (300 MBq and 700 MBq Tc-99m tetrofosmin for stress and rest respectively). All images were acquired on a double head system and were gated using 8 frames, 25 s per frame. ED and ES volumes increased after adenosine but decreased after treadmill resulting in the post-stress LVEF being significantly greater than after adenosine, 60 ± 11 v. 51 ± 13% (p < 0.01). This was caused by the smaller post-stress ESV in the treadmill group 40 ± 20 v. 51 ± 34, p < 0.05. In non-ischaemic scans the LVEF was greater (61± 8 v. 51 ± 14, p < 0.01) and EDV and ESV smaller after both stress and rest. The adenosine test may have an opposite influence on the EDV and ESV in comparison to the submaximal treadmill test and therefore the left ventricular function measurements after adenosine infusion should be interpreted carefully and may not represent those acquired after physical exercise. In the gated SPET scans showing ischaemia the post-stress EDV and ESV may be greater and the LVEF lower than at rest. (author)

  12. /sup 99m/Tc-aminohexylidendiphosphonate and /sup 99m/Tc-pyrophosphate in the scintigraphic diagnosis of experimental myocardial infarction in dogs

    Energy Technology Data Exchange (ETDEWEB)

    Duska, F.; Hadas, L.; Vizda, J.; Kafka, P.; Urbanova, E.; Palicka, V.; Mazurova, Y.; Kuba, M.; Grossman, V.

    1988-10-01

    /sup 99m/Tc-aminohexylidendiphosphonate (/sup 99m/Tc-AHDP) is a new Czechoslovak pharmaceutical of the phosphonate line which contains the amino group NH/sub 2/ in its molecule. This substance was tested in 5 dogs with experimentally provoked 48-h old myocardial infarction. The in-vivo scan and the radioactivity of tissue samples demonstrated that /sup 99m/Tc-AHDP is as suitable for imaging acute myocardial infarction as is the commonly used /sup 99m/Tc-pyrophosphate.

  13. Analysis of 99mTc-labeled radiopharmaceuticals by high-performance liquid chromatography

    International Nuclear Information System (INIS)

    High-performance liquid chromatography (HPLC) equiped with on line radiometric and optical detectors (i.e. radio-HPLC) have been applied to the radiochemical analysis of commonly-used 99mTc-radio pharma ceuticals with a view point to check the radiochemical purities of the compounds. Chromatographic conditions were determined by examination of the types of column, mobile phase and pH. An aqueous size-exclusion (Shim-pack Diol-300) and reversed-phase column (Zorbax-ODS) were found to be suitable for 99mTc-HSA and the other 99mTc-agents, respectively. The analysis of low molecular weight 99mTc-agents (e.g. 99mTc-DTPA, 99mTc-DMSA, 99mTc-pyrophosphate, 99mTc-phytic acid, 99mTc-MDS, 99mTc-HMDP) were done by reversed-phaseion pairing chromatography using a optimized mobile phase consisted on a mixture of 50 mM phosphate buffer (pH 7.0) and 2 mM TBA (tetra nbutyl) ammonium hydroxide) in 30 % methanol. The mobile phases for analysis of medium molecular weight 99mTc-HSA were consisted of a mixture of 50 mM phosphate buffer (ph 7.0) in 30 % methanol, and a mixtures of 1 % SDS (sodium dodecyl sulfonate) in Tris buffer (pH. 7.0), respectively. It was apparent from the radio-chromatograms obtained from these chromatographic conditions, that impurity of 99mTcO4 was observed in 99mTc-pyrophosphate, 99mTc-phytic acid, 99mTc-MDP, 99mTc-HMDP, and impurities of 99mTc-labeled species and 99mTcO4, were observed in 99mTc-HIDA, 99mTc-HIDA, 99mTc-HSA. The radiochemical impurities of the 99mTc-radiopharmaceuticals were ranged between 90 and 100 %. From these results, radio-HPLC has been shown to be suitable method for analysis of 99mTc-radiopharmaceuticals, with rapidity and excellent precision. (author)

  14. Study on the preparation of 99mTc=3- bromo-2, 4, 6-Trimethylacetanilidoiminodiacetic Acid (Br-IDA) as a hepatobiliary imaging agent

    International Nuclear Information System (INIS)

    Br-IDA compound is used for in vivo diagnotic application in nuclar medicine as hepatobiliary imaging agent after being labelled with 99m Tc. A high labelling yield (>97%) and a high in vitro stability (= 95% after 5 h) were achieved by reduction of heptavalent 99m TcO4 which eluted under aseptic conditions from commercially available 99 Mo/99m Tc generator. The stannous reduction method for formation of 99m Tc-Br-IDA complex was used. The different parameters affecting the labelling yield are studied in order to optimize the labelling yield. These factors are the amount of ligand, the Sn(II) content, the pH of reaction mixture and reaction time. The radiochemical purity of 99m Tc-Br-IDA complex was examined by high performance liquid chrimatography (HPLC) and instant thin layer chromatography (ITLC). 99m Tc-Br-IDA complex was rapidly excreted via the hepatocytes with fast liver clearance and minimum urinary excretion (>75% of the activity was found in the gastrointestinal system and < 5% in the kidney, at one hour post injection in mice)

  15. Technetium-99m as alternative to produce somatostatin-labeled derivatives: comparative biodistribution evaluation with {sup 111}In-DTPA-octreotide

    Energy Technology Data Exchange (ETDEWEB)

    Melo, Ivani B.; Buchpiguel, Carlos Alberto, E-mail: ivani@hcnet.usp.br [Universidade de Sao Paulo (USP/LIM43), SP (Brazil). Centro de Medicina Nuclear. Departamento de Radiologia; Ueda, Laura T.; Araujo, Elaine B. de; Muramoto, Emiko; Barboza, Marycel F. de; Mengatti, Jair; Silva, Constancia P.G. da, E-mail: ebaraujo@ipen.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Centro de Radiofarmacia

    2008-07-01

    Synthetic somatostatin (SST) analogues have been used in the preparation of receptor-specific radiopharmaceuticals for diagnostic and therapy of neuroendocrine (NE) tumors. {sup 111}In-DTPA-Octreotide (OctreoScan®) has found useful for imaging a range of tumors, including NE cancer, carcinoide and lymphoma. Unfortunately, {sup 111}In is a high-cost cyclotron produced radioisotope with gamma emission not so suitable for scintigraphic images and for dosimetry like {sup 99m}Tc. This work studied the labeling conditions with {sup 99m}Tc and biological distribution in Swiss mice of two SST analogs (HYNIC-Tyr{sup 3}-Octreotide and HYNICTyr{sup 3}- Octreotate) and compared the biodistribution pattern with {sup 111}In-DTPA-Octreotide. {sup 99}mTc-HYNIC-Tyr{sup 3}- Octreotate ({sup 99m}Tc-HYNIC-TATE) and {sup 99m}Tc-HYNIC-Tyr{sup 3}-Octreotide ({sup 99m}Tc-HYNIC-OCT) were produced by labeling conditions using tricine and EDDA as coligands. {sup 111}In-DTPA-Octreotide ({sup 111}In-DTPA-OCT) was produced by labeling DTPA-Octreotide with {sup 111}InCl{sub 3} (Nordion). Radiochemical purity of labeled preparations was determined by ITLC-SG. Biological distribution studies were performed after injection of radiopharmaceuticals on Swiss mice. Labeling procedures resulted on high radiochemical yield for all three preparations and the labeled products presented high in vitro stability. Biological distribution studies evidenced similar general biodistribution of {sup 99m}Tc-labeled peptides when compared with indium-labeled peptide with fast blood clearance and elimination by urinary tract. Kidneys uptake of {sup 99}mTc-HYNIC-TATE are similar to {sup 111}In-DTPA-Octreotide, and both are significantly higher than {sup 99}mTc-HYNIC-OCT. All labeled peptides presented similar uptake on liver, but the retention in time at intestines, particularly at large intestine, was more expressive for {sup 111}In-labeled peptide. The %ID of {sup 99m}Tc-HYNIC-OCT and {sup 99m}Tc-HYNIC-TATE in

  16. Mononuclear and dinuclear complexes of sup(99m)Tc

    Energy Technology Data Exchange (ETDEWEB)

    Yokoyama, A.; Horiuchi, K. (Kyoto Univ. (Japan). Faculty of Pharmaceutical Science)

    1982-10-01

    In the labelling of penicillamine (pen) with sup(99m)Tc, various complexes were detected. Studies of the conditions for differentiating the product formed in order to understand the chemical and biological behavior were carried out. Marked roles of pH and SnCl/sub 2/ concentration were observed. Controlled delivery of Sn/sup 2 +/ ion was obtained with the tin-adsorbed resin (Sn-resin) developed. Radiopharmaceutical and biological characterization of mononuclear and dinuclear complexes of Tc-Pen, under given sets of labeling conditions, were established. Those studies provided a good basis in technetium chemistry which facilitated the development of new Tc-radiopharmaceuticals. Selection of the appropriate ligand offered a stable mononuclear complex of Tc-bleomycin for tumor detection. Tc-Pen-ethyl-ester for blood-element labeling and, above all, development of a bis-thiosemicarbazone derivative as a new bifunctional chelating agent to be coupled to proteins, fatty acids, glucose etc. On the other hand, various cholescintigraphic agents were better conceived of as dinuclear complexes and this created the possibility of generating a new agent, a phthalein derivative, Tc-Pc containing the required coordination environment for the technetium to be bridged in a dinuclear state.

  17. Mononuclear and dinuclear complexes of /sup 99m/Tc

    Energy Technology Data Exchange (ETDEWEB)

    Yokoyama, A.; Horiuchi, K.

    1982-10-01

    In the labelling of penicillamine (Pen) with /sup 99m/Tc, various complexes were detected. Studies of the conditions for differentiating the product formed in order to understand the chemical and biological behavior were carried out. Marked roles of pH and SnCl/sub 2/ concentration were observed. Controlled delivery of Sn/sub 2//sup +/ ion was obtained with the tin-adsorbed resin (Sn-resin) developed. Radiopharmaceutical and biological characterization of mononuclear and dinuclear complexes of Tc-Pen, under given sets of labelling condition, were established. Those studies provided a good basis in technetium chemistry which facilitated the development of new Tc-radiopharmaceuticals. Selection of the appropriate ligand offered a stable mononuclear complex of Tc-bleomycin for tumor detection, Tc-Pen-ethyl-ester for blood-element labeling and, above all, development of a bis-thiosemicarbazone derivative as a new bifunctional chelating agent to be coupled to proteins, fatty acids, glucose etc. On the other hand, various cholescintigraphic agents were better conceived of as dinuclear complexes and this created the possibility of generating a new agent, a phthalein derivative, Tc-PC, containing the required coordination environment for the technetium to be bridged in a dinuclear state.

  18. Dynamic 99mTc-DTPA radioaerosol lung scanning

    International Nuclear Information System (INIS)

    51 patients (10 normal subjects and 41 pathological ones) underwent dynamic 99mTc-DTPA radioaerosol lung scanning to evaluate different technical aspects of alveolar-capillary permeability, as reflected by the rate constant (k) of the radiotracer exponential washout. A significant difference (p<0.01) was found between normal and pathological subjects but not among pathological groups as a damage of alveolar-capillary barrier is a non specific event common to different lung conditions. No significant correlation was found between total lung k and either spirometric tests (FEV1 and MEF75) or radioaerosol initial distribution, proving thus that the alveolar-capillary permeability is not related to derangements of ventilation. Inter and intraoperator reproducibilities were fairly satisfactory, showing correlation coefficients of 0.992 and 0.996, respectively. Sensitivity and specificity for the diagnosis of increased alveolar-capillary permeability were calculated using different criteria. The best results in the patients studied were achieved by using total rather than regional ks, leading to a specificity of 90% and a sensitivity of 86%. (orig.)

  19. Tc 99m - scorpion venom: labelling, biodistribution and scintiimaging

    International Nuclear Information System (INIS)

    Labelling of scorpion (Mesobuthus tamulus concanesis Pocock) venom was successfully achieved with Tc 99m using direct tin reduction procedure. Biodistribution studies were carried out in Wistar rats at different time intervals after i.v. administration of the labelled venom. Scintiimages were obtained after scorpion envenoming using a large field of view gamma camera to ascertain the pharmacological action of venom in the body. Within 5 min of administration, labelled venom was found in the blood (27.7%), muscle (30.11%), bone (13.3%), kidneys (11.5%), liver (10.4%) and other organs. The level of venom in the kidneys was higher than in the liver. The labelled venom was excreted through renal and hepatobiliary pathways. An immunoreactivity study was carried out in rabbits after i.v. injection of labelled scorpion venom followed by the injection of the species specific antivenom. A threefold increase in uptake by the kidneys ss was observed compared with that seen with scorpion venom alone. the neutralisation of the venom in the kidneys was higher than in the liver. (author)

  20. Tc 99m - scorpion venom: labelling, biodistribution and scintiimaging

    Energy Technology Data Exchange (ETDEWEB)

    Murugesan, S.; Noronha, O.P.D.; Samuel, A.M. [Bhabha Atomic Research Centre, Mumbai (India). Tata Hospital Annexe. Radiation Medicine Center; Murthy, K. Radha Krishna [Seth G.S. Medical College, Mumbai (India). Dept. of Physiology

    1999-07-01

    Labelling of scorpion (Mesobuthus tamulus concanesis Pocock) venom was successfully achieved with Tc 99m using direct tin reduction procedure. Biodistribution studies were carried out in Wistar rats at different time intervals after i.v. administration of the labelled venom. Scintiimages were obtained after scorpion envenoming using a large field of view gamma camera to ascertain the pharmacological action of venom in the body. Within 5 min of administration, labelled venom was found in the blood (27.7%), muscle (30.11%), bone (13.3%), kidneys (11.5%), liver (10.4%) and other organs. The level of venom in the kidneys was higher than in the liver. The labelled venom was excreted through renal and hepatobiliary pathways. An immunoreactivity study was carried out in rabbits after i.v. injection of labelled scorpion venom followed by the injection of the species specific antivenom. A threefold increase in uptake by the kidneys ss was observed compared with that seen with scorpion venom alone. the neutralisation of the venom in the kidneys was higher than in the liver. (author)

  1. Clinical evaluation of 99mTc-pseudogas (Technegas)

    International Nuclear Information System (INIS)

    99mTc-pseudogas (Technegas)--a new agent for lung ventilation scintigraphy--was studied in 6 healthy controls and 42 patients with various pulmonary diseases. Technegas easily enabled the obtaining of multiple planar images and even SPECT images. Its image was superior to that of 133Xe gas or 81mKr gas. We classified its posterior deposition patterns into 6 patterns (Type A: normal distribution; Type B1: mildly irregular distribution; Type B2 : severely irregular distribution; Type C: severely irregular distribution mingled with hot spots; Type D: non hilar hot spots; and Type E: hilar hot spots). The deposition patterns showed mainly Type B1 in restrictive pulmonary diseases and Type B2, C and D in obstructive pulmonary diseases. Further, the deposition patterns correlated well with %FEV1.0, which was a good indicator of the severity of both obstructive and restrictive pulmonary diseases. No adverse reaction was found. We concluded that Technegas is a safe and very useful agent for ventilation study. (author)

  2. Uptake and localization of sup(99m)technetium-methylene-diphosphonate in bone

    International Nuclear Information System (INIS)

    The author investigated the uptake and localization of 99m-technetium-methylene-diphosphonate (99m-Tc-MDP) in bone, to develop a sensitive mean for the detection of early osseous disease. In an electrolysis procedure without the presence of contaminating reductants a 99m-Tc-MDP complex is formed with clear bone-seeking properties. The scans performed in experimental animals are comparable in quality with 99m-Tc(Sn)-MDP scans. The uptake of 99m-Tc-MDP is faster and higher than the uptake of reduced hydrolyzed 99m-Tc. Uptake of 99m-Tc(Sn)-MDP in bone can only take place after decomposition of the complex. As 99m-Tc-MDP is taken up as a unit, this may be a better agent to evaluate the osteoblastic activity in the skeleton. (Auth./R.B.)

  3. /sup 99m/Tc-HIDA dynamic scintigraphy for the diagnosis of gastroesophageal reflux of bile

    Energy Technology Data Exchange (ETDEWEB)

    Bortolotti, M.; Bersani, G.; Labo, G.; Abbati, A.; Turba, E.; Pozzoto, R.

    1985-06-01

    In 5 patients with partial gastric resection and esophagitis, in whom esophageal pH metry was unable to demonstrate significant gastroesophageal reflux (GER), we administered i.v. 5 mCi (185 MBq) of /sup 99m/Tc-HIDA, the patient lying under a computer-assisted LFOV gamma camera. When gallbladder image was evident, caerulein was administered i.v. at a physiologic dose in order to induce gallbladder contraction, and in the subsequent 45 min the patient was asked to perform a standard series of manoeuvres that increase the intraabdominal pressure (Valsalva, etc.) and favour GER. Scintigraphic images and time/activity curves obtained from areas of interest corresponding to gastric remnant and distal esophagus showed that at least one of these manoeuvres in each case was followed by the appearance of the radiocompound in the distal esophagus, indicating a /sup 99m/Tc-HIDA-tagged bile GER. Consequently, we believe that HIDA-GER dynamic scintigraphy may be more useful than esophageal pH metry in demonstrating the bilairy origin of an esophagitis.

  4. sup(99m)Tc-HIDA dynamic scintigraphy for the diagnosis of gastroesophageal reflux of bile

    International Nuclear Information System (INIS)

    In 5 patients with partial gastric resection and esophagitis, in whom esophageal pH metry was unable to demonstrate significant gastroesophageal reflux (GER), we administered i.v. 5 mCi (185 MBq) of sup(99m)Tc-HIDA, the patient lying under a computer-assisted LFOV gamma camera. When gallbladder image was evident, caerulein was administered i.v. at a physiologic dose in order to induce gallbladder contraction, and in the subsequent 45 min the patient was asked to perform a standard series of manoeuvres that increase the intraabdominal pressure (Valsalva, etc.) and favour GER. Scintigraphic images and time/activity curves obtained from areas of interest corresponding to gastric remnant and distal esophagus showed that at least one of these manoeuvres in each case was followed by the appearance of the radiocompound in the distal esophagus, indicating asup(99m)Tc-HIDA-tagged bile GER. Consequently, we believe that HIDA-GER dynamic scintigraphy may be more useful than esophageal pH metry in demonstrating the bilairy origin of an esophagitis. (orig.)

  5. Pulmonary clearance of radiotracers after positive end-expiratory pressure or acute lung injury

    International Nuclear Information System (INIS)

    In anesthetized rabbits we measured clearance from lung to blood of eight aerosolized technetium-99m-labeled compounds: diethylenetriaminepentaacetate (99mTc-DTPA); cytochrome c; myoglobin; a myoglobin polymer; albumin; and anionic, cationic, and neutral dextrans of equivalent molecular size. We investigated the effect of applying positive end-expiratory pressure (PEEP) and, on a subsequent occasion, of injecting oleic acid intravenously to produce acute lung injury on the pulmonary clearance rate. Base-line clearance rates were monoexponential and varied with the molecular weights of the radiotracers. For each tracer the rate of clearance was increased a similar degree by either PEEP or oleic acid. However, with PEEP, clearance remained monoexponential, whereas after oleic acid, smaller molecular-weight radiotracers had multiexponential clearance curves. This suggests that after oleic acid the alveolar epithelium breaks down in a nonuniform fashion. We conclude that differentiation of the effect of PEEP from that of severe lung injury caused by oleic acid is not readily accomplished by either increasing the size of the tracer molecule or by varying the molecular charge

  6. Comparative myocardial uptake of technetium-99 m sestamibi and technetium-99m tetrofosmin one hour after stress injection

    International Nuclear Information System (INIS)

    Technetium-99m sestamibi and 99mTc-tetrofosmin are at present the preferred tracers for simultaneous assessment of myocardial perfusion and function by gated single-photon emission tomography (SPET). The aim of this work was to compare sestamibi and tetrofosmin myocardial uptake 1 h after stress injection. Consecutive unselected patients were studied either with sestamibi or with tetrofosmin on a random basis, until at least 100 patients had been enrolled for each gender and tracer. Stress was obtained by dipyridamole or exercise or combined dipyridamole + exercise; in the latter cases, exercise was sustained for at least 1.5 min after tracer injection. Injected activity was similarly adjusted to body weight. For each patient, imaging began 60-75 min after injection. All SPET projections were summed; due to the acquisition technology (''roving zoom'', i.e. a mobile zoom), the heart always appeared at the centre of the frame in all projections and in the sum image. Thus minimal lung background contamination could be assumed in an elliptic region of interest placed over the heart on the sum image. Three indexes were analysed: total myocardial counts (Sum), mean myocardial pixel (Mean) and maximum myocardial pixel (Max). Four patient groups were analysed: males with sestamibi or tetrofosmin (MS: n = 189 and MT: n = 157), females with sestamibi or tetrofosmin (FS: n = 101 and FT: n = 104). MS and MT groups were comparable for physical variables, maximum heart rate and stress type, as were the FS and FT groups. Sum, Mean and Max were significantly higher with sestamibi (P = 0.0001 by ANOVA). Comparing MS vs MT and FS vs FT, mean values ± SD were as follows: for Sum (kcounts) 750±184 vs 652±166, and 707±202 vs 594±189; for Mean (counts) 4517±1171 vs 4107±898, and 4908±1119 vs 4144±1025; and for Max (counts) 6471±1654 vs 5794±1312, and 7318±1886 vs 6152±1684. The mean gain with sestamibi was +15%, +10% and +12% in males, and +19%, +18% and +19% in females

  7. The quality control of technetium-99m radiopharmaceuticals produced at the AAEC Research Establishment

    International Nuclear Information System (INIS)

    The methods of quality control used for technetium-99m radiopharmaceuticals produced at the AAEC Research Establishment are described for both non-fission and fission derived sources of sodium pertechnetate, technetium-99m labelled radipopharmaceuticals, and reagent kits produced for technetium-99m labelling

  8. Patient dosimetry of intravenously administered 99mTc-annexin V

    NARCIS (Netherlands)

    Kemerink, G J; Liem, I H; Hofstra, L; Boersma, H H; Buijs, W C; Reutelingsperger, C P; Heidendal, G A

    2001-01-01

    UNLABELLED: Annexin V labeled with 99mTc is evaluated as a potential in vivo marker for tissue with increased apoptosis. Promising results in patients have been obtained with 99mTc-(n-1-imino-4-mercaptobutyl)-annexin V (99mTc-i-AnxV). Because information on biodistribution and radiation burden is de

  9. Combined protocol of 99mTc-Ciprofloxalina and 99mTc-MDP in the diagnosis of complicated osteomyelitis and infected prosthesis

    International Nuclear Information System (INIS)

    Introduction: Bone scintigraphy with 99mTc-MDP is a useful technique in the diagnosis of osteomyelitis, however, has limited specificity in the presence of previous bone pathology (complicated osteomyelitis). 99mTc-ciprofloxacin is one of the most widely used radiotracers for the detection of bone infection, although controversies persist on its diagnostic performance. Objective: To determine the clinical value of 99mTc-ciprofloxacin/99mTc-MDP combined protocol in the diagnosis of complicated osteomyelitis and infected joint prosthesis. Materials and methods: 37 patients with clinically suspected complicated osteomyelitis or infected prosthesis were studied with 99mTc-ciprofloxacin and 99mTc-MDP scintigraphy. 26/37 patients had previous fractures, 7 had knee replacements and 4 had hip replacements. All of the patients underwent clinical and bacteriological follow-up. Results: The method presented sensitivity of 94%, 79% specificity, 81% positive predictive value and 94% negative predictive value, with an accuracy of 86%. Conclusions: The combined protocol using 99mTc-ciprofloxacin/99mTc-MDP showed high diagnostic performance in complicated osteomyelitis and infected joint prosthesis

  10. The influence of experimental conditions on the preparation of 99mTc-pyrrolidino-methyl tetracycline [99mTc-PMT] in the form of an instant kit

    International Nuclear Information System (INIS)

    The effects of varying the experimental conditions of the preparation of the renal scanning agent 99mTc-PMT on its organ distribution have been investigated. The stability of 99mTc-PMT was determined in vitro. A procedure for the preparation of this agent is recommended. (orig.)

  11. Sup (99m) Tc-MDP accumulation and absence of concentration of sup (99m) Tc-sulfur colloid in the spleen

    International Nuclear Information System (INIS)

    A case of a nine years old girl with sickle cell anemia, that showed splenic accumulation of sup (99m) Tc-MDP during bone scintigraphy is reported. On the other side, the scan with sup (99m) Tc-sulfur colloid showed no uptake in the spleen. These findings are discussed with a brief review of the pertinent literature. (Author)

  12. (99m)Tc-Galacto-RGD2: a novel 99mTc-labeled cyclic RGD peptide dimer useful for tumor imaging.

    Science.gov (United States)

    Ji, Shundong; Czerwinski, Andrzej; Zhou, Yang; Shao, Guoqiang; Valenzuela, Francisco; Sowiński, Paweł; Chauhan, Satendra; Pennington, Michael; Liu, Shuang

    2013-09-01

    This study sought to evaluate [(99m)Tc(HYNIC-Galacto-RGD2)(tricine)(TPPTS)] ((99m)Tc-Galacto-RGD2: HYNIC = 6-hydrazinonicotinyl; Galacto-RGD2 = Glu[cyclo[Arg-Gly-Asp-D-Phe-Lys(SAA-PEG2-(1,2,3-triazole)-1-yl-4-methylamide)

  13. Noninvasive quantitation of myocardial infarction with technetium-99m pyrophosphate

    International Nuclear Information System (INIS)

    We sought to quantitate infarct size using radioactive imaging techniques. Infarcts were created in closed chest dogs. Using a scintillation camera interfaced to a computer, infarct images were made in the anterior, left lateral, LAO, and RAO projections, 48 hours after infarction and 75 to 90 min following the intravenous injection of 15 mCi of technetium-99m pyrophosphate (Tc-PYP). Images were computer enhanced and area was calibrated with a radioactive grid source of known dimensions. Image radioactivity was normalized for decay and dose corrected for body weight. Animals were sacrificed two hours following the injection of Tc-PYP. Postmortem images were also computer enhanced and calibrated. Gross infarct area and weight were estimated and transmural biopsies were evaluated for Tc-PYP activity and analyzed for creatine phosphokinase (CPK) content. Contiguous biopsies were pathologically analyzed and graded. There was a negative correlation between tissue Tc-PYP activity and CPK content (r = -0.89). Pathologic severity worsened with increased Tc-PYP activity and diminished CPK content. There was a good correlation between gross infarct area and image infarct area, both in vivo (r = 0.79), and at post-mortem examination (r = 0.95). Gross infarct weight also correlated well with image infarct activity in vivo (r = 0.83 in the RAO view) and at postmortem examination (r = 0.87). An additional correlation between gross infarct weight and in vivo image infarct area (r = 0.92 in the LAO view) appeared most promising for future clinical evaluation. These experimental relationships are analyzed and future patient application of these imaging techniques are considered

  14. Technetium-99m-sestamibi scintigraphy in breast nodes; Estudo cintilografico de nodulos mamarios com tecnecio-99m-sestamibi

    Energy Technology Data Exchange (ETDEWEB)

    Pellini, Marcos Pinto; Fonseca, Lea Mirian Barbosa da [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina; Balen, Jacir Luiz; Fabricio, Maria Ines Menescal [Universidade Federal, Rio de Janeiro, RJ (Brazil). Faculdade de Medicina. Inst. de Ginecologia; Amarante Junior, Jose Luiz de Medeiros

    1997-01-01

    The purpose was to was to determine if technetium-99m-sestamibi accumulates preferentially within the malignant palpable nodes of breast. Twenty-five patients, mean age 36.16 ({+-} 9.34) year, and without any other additional information, underwent breast scintigraphy and excisional biopsy. We had nineteen true-negative cases, four true-positive, two false-positive and none false-negative. Sensitivity, 100% specificity, 90.5%, accuracy, 92%; PPV, 66.7%, NPV, 100%. The four true positive were invasive intraductal carcinomas and one of them metastases in auxiliary lymph-nodes, confirmed in biopsy and well defined in scintigraphy. The two false-positives were a fibroadenoma with high cellularity and a fibrodisplasy surrounded by chronic inflammatory process. Two statistical tests were applied: chi-square and Fisher. Both rejected the antithesis at a confidence interval of 99% (p , 0.01) We concluded that technetium-99-sestamibi accumulates preferentially within the malignant nodes of breast. (author) 17 refs., 3 figs., 2 tabs.

  15. /sup 99m/Tc-aminohexylidendiphosphonate and /sup 99/mTc-Pyrophosphate in the scintigraphic diagnosis of experimental cardiomyopathy in dogs

    Energy Technology Data Exchange (ETDEWEB)

    Duska, F.; Kafka, P.; Mazurova, Y.; Hadas, L.; Vizda, J.; Palicka, V.; Grossman, V.

    1987-10-01

    Experimental cardiomyopathy was provoked in 24 dogs with high intravenous doses of adrenaline and theophylline. These lesions were studied by means of the new agent /sup 99m/Tc-AHDP and /sup 99m/Tc-PYP in comparison. Cardiomyopathy could be imaged as early as 4 h after the onset of involvement but not later than 7 days. A maximum accumulation occurred in lesions 24 h old, /sup 99m/Tc uptake in the myocardium was graded scintigraphically, /sup 99m/Tc-AHDP was accumulated in the altered myocardium to a greater extent than /sup 99m/Tc-PYP. Scintigraphic findings were in good agreement with plasma levels of creatine-kinase. A comparison with histology demonstrated that the maximum accumulation of radiopharmaceuticals occurred at the time when the development of myocardium involvement reached the stage of myocytolysis.

  16. Preparation and in vivo evaluation of 99mTcN-tertiary butyl xanthate as a potential myocardial agent

    International Nuclear Information System (INIS)

    99mTc-tertiary butyl isonitrile (TBI), a complex structurally similar to 99mTc-sestamibi, has been reported to be taken up in the myocardium, albeit with significant retention in liver and lungs, thereby leading to obscure heart images when used for in vivo imaging. In the present study, tert-butyl xanthate, which is similar to TBI, but possesses two sulphur donors, has been synthesized in excellent yield by reacting tert-butanol with carbon disulphide and sodium hydroxide in dry ether. The prepared ligand was then labeled with [99mTcN]2+ core and the resultant complex was characterized by paper electrophoresis and HPLC. The complex was obtained in more than 95% yield and was found to be neutral. The preparation was evaluated in Swiss mice for its myocardial perfusion characteristics. The maximum heart uptake observed was 2.34%ID/g at 5 min p.i., which was cleared rapidly, with retention of 0.50%ID/g of the activity at 60 min p.i. However, owing to slow clearance from blood, liver and lungs, the heart/blood, heart/liver and heart/lung ratios at all the time points of study were not significantly high

  17. An oestrogen-derivative labeled with 99mTc and its radiopharmaceutical potential

    International Nuclear Information System (INIS)

    An oestrogen derivative 3,17-α-oestradiolyl propyl 1,4,8,11-tetraazacyclotetradecanyl-1-(4-methylbenzoic acid)ester (ESTCPTA) that is 3,17-α-oestradiolyl propinol coupled to 1-(4-methylbenzoic acid)1,4,8,11-tetraazacyclotetradecane (CPTA) was synthesized in five steps. The product was purified by recrystallization in ethyl alcohol, and analysed by NMR and IR spectroscopy. ESTCPTA was labeled with 99mTc and radio thin layer chromatography (RTLC) and radio-paper electrophoresis were used to determine the radiochemical yields. Specific activity was approximately 23.7 GBq/mmol and the labeling yield was over 95%. The biodistribution studies were performed on female Albino Wistar rats. The rats were sacricified by ether narcotization at certain time intervals and the activity of the organs was counted by a gamma counter. The activity per gram tissue was calculated and time activity curves were generated. (author)

  18. Biological in vitro and in vivo studies of a series of new asymmetrical cationic [99mTc(N)(DTC-Ln)(PNP)]+ complex (DTC-Ln = alicyclic dithiocarbamate and PNP = diphosphinoamine).

    Science.gov (United States)

    Bolzati, Cristina; Cavazza-Ceccato, Mario; Agostini, Stefania; Refosco, Fiorenzo; Yamamichi, Yoshihiro; Tokunaga, Shinji; Carta, Davide; Salvarese, Nicola; Bernardini, Daniele; Bandoli, Giuliano

    2010-05-19

    (99m)Tc(N)-DBODC5 is a cationic mixed compound under clinical investigation as potential myocardial imaging agent. In spite of this, analogously to the other cationic (99m)Tc-agents, presents a relatively low first-pass extraction. Thus, modification of (99m)Tc(N)-DBODC(5) direct to increase its first-pass extraction keeping unaltered the favorable imaging properties would be desirable. This work describes the synthesis and biological evaluation of a series of novel cationic (99m)Tc-nitrido complexes, of general formula [(99m)TcN(DTC-Ln)(PNP)](+) (DTC-Ln= alicyclic dithiocarbamates; PNP = diphosphinoamine), as potential radiotracers for myocardial perfusion imaging. The synthesis of cationic (99m)Tc-(N)-complexes were accomplished in two steps. Biodistribution studies were performed in rats and compared with the distribution profiles of (99m)Tc(N)-DBODC5 and (99m)Tc-Sestamibi. The metabolisms of the most promising compounds were evaluated by HPLC methods. Biological studies revealed that most of the complexes have a high initial and persistent heart uptake with rapid clearance from nontarget tissues. Among tested compounds, 2 and 12 showed improved heart uptake with respect to the gold standard (99m)Tc-complexes with favorable heart-to-liver and slightly lower heart-to-lung ratios. Chromatographic profiles of (99m)Tc(N)-radioactivity extracted from tissues and fluids were coincident with the native compound evidencing remarkable in vivo stability of these agents. This study shows that the incorporation of alicyclic dithiocarbamate in the [(99m)Tc(N)(PNP)](+) building block yields to a significant increase of the heart uptake at early injection point suggesting that the first-pass extraction fraction of these novel complexes may be increased with respect to the other cationic (99m)Tc-agents keeping almost unaltered the favorable target/nontarget ratios. PMID:20402465

  19. Application of extraction generator for preparation of radiopharmaceuticals on 99mTc base

    International Nuclear Information System (INIS)

    Data on extraction generator use for preparation of high-purity 99mTc of 99Mo are presented, quality of 99mTc solution is investigated. Extractant (methyl ethyl ketone) radiolysis products are studied. High degree of purification of 99mTc eluate from extraction generator both of organic and inorganic impurities is achieved. This fact is a guarantee of high quality of radiopharmaceuticals prepared using this 99mTc solution. 99mTc solution obtained from extraction generator surpasses by quality analogues solution from sorption generator. In the case of use of centrifugal extractor for 99Mo and 99mTc separation concentration of methyl ethyl ketone in 99mTc solution is not more 0.04 mg/ml

  20. Investigation of four {sup 99m}Tc-labeled bacteriophages for infection-specific imaging

    Energy Technology Data Exchange (ETDEWEB)

    Rusckowski, Mary [Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655-0243 (United States)], E-mail: mary.rusckowski@umassmed.edu; Gupta, Suresh; Liu Guozheng; Dou Shuping; Hnatowich, Donald J. [Division of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655-0243 (United States)

    2008-05-15

    Introduction: This study investigated radiolabeled bacteriophages for specific detection of infection through gamma imaging. Previously, a {sup 99m}Tc-labeled M13 phage demonstrated specific binding for its host Escherichia coli in vitro and in mice through imaging. Methods: This study was extended to phages P22, E79, VD-13 and phage 60. Each was radiolabeled with {sup 99m}Tc using the chelator MAG{sub 3}, and were evaluated for binding to host and non-host bacteria in vitro and in a mouse infection model. Results: In vitro, each {sup 99m}Tc-phage bound to its host at least 4-fold higher than to non-host bacteria. For example, {sup 99m}Tc-E79 showed 10- to 20-fold greater binding to host Pseudomonas aeruginosa compared to non-host Escherichia coli and Salmonella enterica, and {sup 99m}Tc-phage 60 showed 20-fold greater binding to host Klebsiella pneumoniae over non-hosts. Mice received host or non-host bacteria in one thigh, and 3 h later, the {sup 99m}Tc-phages were administered intravenously. After a further 3 h, the tissues were counted. Liver accumulation was highest for {sup 99m}Tc-E79, averaging 39% compared to an average of 13% for the other {sup 99m}Tc-phages. Animals infected with host bacteria showed infected thigh/normal thigh ratios of 14.2 for {sup 99m}Tc-E79, 2.9 for {sup 99m}Tc-P22, 3.5 for {sup 99m}Tc-VD-13 and 2.1 for {sup 99m}Tc-phage 60. Conclusions: Although specific host binding was observed in vitro for each of these four {sup 99m}Tc-phages, only {sup 99m}Tc-E79 showed specificity for its host in an in vivo model.

  1. 99mTc-DTPA Renal Dynamic Imaging Method May Be Unsuitable To Be Used as the Reference Method in Investigating the Validity of CDK-EPI Equation for Determining Glomerular Filtration Rate

    OpenAIRE

    Xie, Peng; Huang, Jian-Min; LIU, XIAO-MEI; Wu, Wei-Jie; Pan, Li-Ping; Lin, Hai-Ying

    2013-01-01

    Objective To compare the measurements of glomerular filtration rate (GFR) determined by 99mTc-diethylene triamine pentaacetic acid (99mTc-DTPA) renal dynamic imaging with those estimated by Chronic Kidney Disease Epidemiology Collaboration (CDK-EPI) equation and to identify a more accurate measurement of GFR of chronic kidney disease (CKD) patients in clinical practice. Methods The GFR was determined simultaneously by 3 methods: (a) dual plasma sample clearance method (tGFR); (b) renal dynami...

  2. Assessment of the direct cyclotron production of (99m)Tc: An approach to crisis management of (99m)Tc shortage.

    Science.gov (United States)

    Rovais, Mohammad Reza Aboudzadeh; Aardaneh, Khosro; Aslani, Gholamreza; Rahiminejad, Ali; Yousefi, Kamran; Boulouri, Fatemeh

    2016-06-01

    Nowadays, the cyclotron production of technetium-99m ((99m)Tc) has been increased, due to the worldwide (99m)Tc generator shortage. In the present work, an improved strategy for the production of (99m)Tc, using the proton irradiation of the enriched (100)Mo was developed. The performance of this method in terms of the production yield, chemical purity, radiochemical purity, as well as radionuclide purity was evaluated. The average production yield was measured to be 356MBqμA(-1)h(-1). A good agreement was found between the calculated production yield and the experimental one. The radiochemical separation and total recovery yields of (99m)Tc were 92% and 69%, respectively. The radiochemical and the radionuclide purities of the (99m)Tc were 99% and >99.99% at the end of purification, respectively. The results of quality control tests (QC) support the concept that cyclotron-produced (99m)Tc is suitable for preparation of USP-compliant.

  3. Using 99mTc-DTPA radioaerosol inhalation lung scan as compared with computed tomography to detect lung injury in blunt chest trauma

    International Nuclear Information System (INIS)

    Detection of pulmonary contusion in patients with blunt chest trauma is very important so as to commence therapy immediately to avoid irreversible damage. The purpose of our study was to evaluate the efficacy of technetium-99m diethylene triamine penta-acetic acid (99mTc-DTPA) aerosol inhalation lung scintigraphy in comparison with chest computed tomography (CT) in the diagnosis of pulmonary contusion at acute blunt chest trauma. Twenty-nine patients with isolated blunt chest trauma were referred to the emergency department of our hospital, and nine healthy people participated in this study. Sixteen patients who had pulmonary contusion on CT scans were referred to as group 1, and 13 patients who had normal CT scans as group 2. Nine healthy people comprised a control group. 99mTc-DTPA aerosol inhalation lung scintigraphy was performed on the first day in all patients. The mean half time (T1/2) and penetration index values of 99mTc-DTPA clearance were significantly lower in groups 1 and 2 compared with the control group. Among the three groups, there were no significant differences in arterial blood gas analysis except for PO2. The mean T1/2 value of 99mTc-DTPA clearance did correlate with PO2 values but not with pH, PCO2, or HCO3 values. 99mTc-DTPA radioaerosol inhalation lung imaging may serve as a useful adjunct and supportive method to chest CT scanning for detecting mild pulmonary contusion. (author)

  4. Regional differences between 99mTc-ECD and 99mTc-HMPAO SPET in perfusion changes with age and gender in healthy adults

    International Nuclear Information System (INIS)

    A number of studies using single-photon emission tomography (SPET) have shown perfusion changes with age in several cortical and subcortical areas, which might distort the results of perfusion imaging studies of neuropsychiatric disorders. Technetium-99m labelled ethyl cysteinate dimer (ECD) and hexamethylpropylene amine oxime (HMPAO) are both used as markers of cerebral perfusion, but have different pharmacokinetics and retention patterns. The aim of this study was to determine whether age and gender effects on perfusion SPET differ depending on whether 99mTc-HMPAO or 99mTc-ECD is used. Forty-five subjects (20 male and 25 female, mean age 52.8±6.6 years) were assigned to 99mTc-HMPAO SPET (HMPAO group), and 39 subjects (24 male and 15 female, mean age 52.6±6.7 years) to 99mTc-ECD SPET (ECD group). SPET images were obtained about 10 min after intravenous injection of approximately 800 MBq 99mTc-HMPAO or 99mTc-ECD using the same SPET scanner. Three-dimensional volumetric magnetic resonance imaging was performed to as7sess morphological changes in the grey matter. All image processing and statistical analyses were performed using SPM99 software. An area in the right anterior frontal lobe showed an increase in perfusion with age only in the HMPAO group, whereas areas in the bilateral retrosplenial cortex showed decreases in perfusion with age only in the ECD group; neither group showed corresponding changes in the grey matter. The present study shows that different effects of age on perfusion are observed depending on whether 99mTc-HMPAO and 99mTc-ECD is used. This suggests that the results of perfusion SPET are differently confounded depending on the tracer used, and that perfusion SPET with these tracers has limitations when used in research on subtle perfusion changes. (orig.)

  5. Mechanism of sup(99m)Tc-methylene diphosphonate (sup(99m)Tc-MDP) localization in experimental studies of bone tumors

    International Nuclear Information System (INIS)

    [Materials and Methods] (I) VX2 carcinoma was transplanted to the rabbit tibia, sup(99m)Tc-MDP was intravenously injected when bone destruction was radiologically seen. Preoperative scintigraphs, postoperative one of the resected tibia and fibra and scintigraphs of sliced tumor were compared with each other. Tissue fragments were collected from the sliced tumor, and RI was measured to compare concentration rates at different parts together with histological investigations. (II) Tetracycline was given to the rabbit of Experiment (I) to compare with the concentration of sup(99m)Tc-MDP. (III) Microautoradiography method was done on the experimental animals for investigation of the concentration of sup(99m)Tc-MDP. [Results] (I) Localization of sup(99m)Tc-MDP was increased at the sites of tumorous bone formation, of reactive bone formation at tumor edges, and of cartilage calcification, with little concentration at tumor cells or necrotic tissue. (II) Deposition sites of tetracycline nearly corresponded with those showing higher concentration of sup(99m)Tc-MDP. (III) microautoradiograms showed high precipitation of silver granules at new bone formation sites. More remarkable localization of sup(99m)Tc-MDP were seen at sites of formation of tumorous bone, of reactive bone at tumor edges and of cartilage calcification. The results deny the view that localization of sup(99m)Tc-MDP was determined by increased blood flow and collagen metabolism, and affirm the view of Galasko et al. that it was determined by new bone formation. Further, results of studies on tetracycline distribution correspond with those of investigations on sup(99m)Tc-MDP localization, indicating that sup(99m)Tc-MDP concentration is strongly determined by tumorous and reactive new bone formation and in cartilage calcification. (author)

  6. Localization of gastrointestinal bleeding: superiority of 99mTc sulfur colloid compared with angiography

    International Nuclear Information System (INIS)

    Preliminary experience with technetium-99m sulfur colloid scintigraphy in 43 patients with lower gastrointestinal bleeding is described. Within minutes of the intravenous introduction of the radiopharmaceutical, a fraction of the injected activity extravasates at the bleeding site and is eliminated from the circulation. Because of rapid clearance of the radiopharmaceutical from the vascular pool by the reticuloendothelial system, a contrast develops between the site of bleeding and surrounding background. Based on animal experiments, bleeding rates as low as 0.05-0.1 ml/min can be detected with this technique. The sensitivity of this technique in the detection of the site of hemorrhage is significantly higher than arteriography or other techniques that use radioactive blood pool indicators. In this study all patients underwent both scintigraphy and arteriography within several hours. In 20 patients with negative scintigrams, arteriography showed no evidence of bleeding. In the other 23 patients with scintigraphic evidence of hemorrhage, arteriograms were positive for bleeding only in 10. In 19 of the 23 patients, a cause for bleeding was eventually established by other means. This technique appears to offer a simple, practical, and reliable approach to the evaluation of patients with lower gastrointestinal bleeding

  7. Localization of gastrointestinal bleeding: superiority of /sup 99m/Tc sulfur colloid compared with angiography

    International Nuclear Information System (INIS)

    Preliminary experience with technetium-99m sulfur colloid scintigraphy in 43 patients with lower gastrointestinal bleeding is described. Within minutes of the intravenous introduction of the radiopharmaceutical, a fraction of the injected activity extravasates at the bleeding site and is eliminated from the circulation. Because of rapid clearance of the radiopharmaceutical from the vascular pool by the rediculoendothelial system, a contrast develops between the site of bleeding and surrounding background. Based on animal experiments, bleeding rates as low as 0.05-0.1 ml/min can be detected with this technique. The sensitivity of this technique in the detection of the site of hemorrhage is significantly higher than arteriography or other techniques that use radioactive blood pool indicators. In this study all patients underwent both scintigraphy and arteriography within several hours. In 20 patients with negative scintigrams, arteriography showed no evidence of bleeding. In the other 23 patients with scintigraphic evidence of hemorrhage, arteriograms were positive for bleeding only in 10. In 19 of the 23 patients, a cause for bleeding was eventually established by other means. This technique appears to offer a simple, practical, and reliable approach to the evaluation of patients with lower gastrointestinal bleeding

  8. Detection of gastrointestinal bleeding with /sup 99m/Tc-sulfur colloid

    International Nuclear Information System (INIS)

    We have developed a single scintigraphic technique to detect and localize the site of bleeding in the gastrointestinal tract. This examination requires only intravenous administration of /sup 99m/Tc sulfur colloid and imaging of the abdomen. Very shortly after the introduction of the radiopharmaceutical, a fraction of the injected activity extravasates at the bleeding site and is eliminated from the circulation. This phenomenon is repeated each time the blood recirculates adding another, but smaller fraction to the extravasated activity at the site of hemorrhage. Because of continued clearance of the radiopharmaceutical from the vascular pool by the reticuloendothelial system, a contrast is eventually reached between the site of bleeding and surrounding background. Based on animal experiments bleeding rates as low as .05-0.1 cc/min can be detected with this technique. The sensitivity of the approach in the detection of the site of hemorrhage is significantly higher than arteriography or other techniques which utilize radioactive blood pool indicators. We have mainly used this technique in the evaluation of patients with GI bleeding. In more than 25 patients with negative scintigrams for hemorrhage, arteriography showed no evidence of bleeding. In 38 patients with scintigraphic evidence of bleeding. In 38 patients with scintigraphic evidence of hemorrhage, arteriograms were positive for bleeding in les than 50% of the cases. No false positive results have been noted in the latter group. This technique appears to offer a simple, practical and reliable approach to the evaluation of patients with gastrointestinal bleeding

  9. Differential uptake of Tc-99m DMSA and Tc-99m EC in renal tubular disorders: Report of two cases and review of the literature

    International Nuclear Information System (INIS)

    Tc-99m DMSA and Tc-99m EC studies are invaluable functional imaging modalities for renal structural and functional assessment. Normally, the relative renal function estimated by the two methods correlates well with each other. We here present two patients with renal tubular acidosis who showed impaired/altered DMSA uptake with normal EC renal dynamic study depicting the pitfall of DMSA imaging in tubular disorders. The two presented cases also depict distinct pattern of Tc-99m DMSA scintigraphic findings in patients with proximal and distal renal tubular acidosis, thus highlighting the factors affecting DMSA kinetics

  10. Dimer of the peptide cycle (Ar-Gly-Asp-D-Phe-Lys) radiolabeled with {sup 99m}Tc for the integrin s over-expression image: formulation, biokinetics and dosimetry; Dimero del peptido ciclo(Arg-Gly-Asp-D-Phe-Lys) radiomarcado con {sup 99m}Tc para la imagen de sobre-expresion de integrinas: formulacion, biocinetica y dosimetria

    Energy Technology Data Exchange (ETDEWEB)

    Ortiz A, Z.

    2013-07-01

    In breast cancer, α(v)β(3) and/or α(v)β(5) integrin s are over-expressed in both endothelial and tumour cells. Radiolabeled peptides based on the RGD (Arg-Gly-Asp) sequence are radiopharmaceuticals with high affinity and selectivity for those integrin s. The RGD-dimer peptide (E-[c(RGDfK)]{sub 2}) radiolabeled with {sup 99m}Tc has been reported as a radiopharmaceutical with 10-fold higher affinity for the α(v)β(3) integrin as compared to the RGD-monomer. EDDA (Ethylenediamine-N,N-diacetic acid) is a hydrophilic molecule that may favours renal excretion when used as coligand in the {sup 99m}Tc labelling of HYNIC-peptides and can easily be formulated in a lyophilized kit. Aim: Establish a biokinetic model for {sup 99m}Tc-EDDA/HYNIC-E-[c(RGDfK)]{sub 2} prepared from lyophilized kits and evaluate the dosimetry as breast cancer imaging agent. Methods: {sup 99m}Tc labelling was performed by addition of sodium pertechnetate solution and 0.2 M phosphate buffer ph 7.0 to a lyophilized formulation containing E-[c(RGDfK)]{sub 2}, EDDA, tricine, mannitol and stannous chloride. Radiochemical purity was evaluated by reversed phase HPLC and ITLC-SG analyses. Stability studies in human serum were carried out by size-exclusion HPLC. In-vitro cell uptake was tested using breast cancer cells (MCF7, T47D and MDA-MB-231) with blocked and non-blocked receptors. Biodistribution and tumour uptake were determined in MCF7 tumour-bearing nude mice with blocked and non-blocked receptors, and images were obtained using a micro-SPECT/CT. Whole-body images from seven healthy women were acquired at 1, 3, 6 and 24 h after {sup 99m}Tc-EDDA/HYNIC-E-[c(RGDfK)]{sub 2} administration obtained with radiochemical purities of >94 %. Regions of interest (ROIs) were drawn around source organs on each time frame. Each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate {sup 99m}Tc-EDDA/HYNIC-E-[c(RGDfK)]{sub 2} time-activity curves in each

  11. Is the renal uptake of 99mTc-DMSA decreased in microalbuminuric diabetic patient?

    International Nuclear Information System (INIS)

    Diabetic nephropathy is the most common cause of end stage renal disease and the incidence is progressively increasing. The aim of this study was to investigate the differences of 99mTc-DMSA renal uptake among diabetic patients with normoalbuminuria, microalbuminuria and overt proteinuria, and then to determine the clinical usefulness of 99mTc-DMSA in predicting early diabetic nephropathy. 99mTc-DMSA scan was performed and a total renal uptake of 99mTc-DMSA was measured in 145 diabetic patients. Patients were divided into 3 groups according to the amount of 24 hour urinary albumin excretion as Group I (normoalbuminuria, 74 cases ), Group II (microalbuminuria, 39 cases), and Group III (overt proteinuria, 32 cases). The differences of 99mTc-DMSA renal uptake among the 3 groups and the correlation between the renal uptake of 99mTc-DMSA and other clinical parameters were analyzed. The total renal uptake of 99mTc-DMSA of Group II (40.8±11.0%) was significantly lower than that of Group I (54.4±6.3%, p99mTc-DMSA total renal uptakes correlated negatively with serum creatinine level (r=0.629, p99mTc-DMSA total renal uptake of diabetic patients with microalbuminuria was significantly decreased compared with that of patients of normoalbuminuria. Therefore, 99mTc-DMSA scan can be used as a diagnostic study for early detection of the diabetic nephropathy

  12. [sup 99m]Tc-ECD SPECT study in dementia and aphasia

    Energy Technology Data Exchange (ETDEWEB)

    Kuwabara, Yasuo; Ichiya, Yuichi; Otsuka, Makoto; Sasaki, Masayuki; Akashi, Yuko; Fukumura, Toshimitsu; Yoshida, Tsuyoshi; Masuda, Kouji; Ichimiya, Atsushi (Kyushu Univ., Fukuoka (Japan). Faculty of Medicine)

    1993-09-01

    We studied clinical significance of [sup 99m]Tc-L,L,-ethyl cysteine dimer ([sup 99m]Tc-ECD) SPECT study in dementia and aphasia, and compared it with [sup 99m]Tc-HMPAO SPECT study. The subjects consisted of 13 patients, including 10 patients with dementia and 3 patients with aphasia. Hypoperfusion areas were detected in 5 out of 10 patients with dementia and 2 out of 3 patients with aphasia in [sup 99m]Tc-ECD SPECT, and in 4 out of 10 patients with dementia and all of 3 patients with aphasia in [sup 99m]Tc-HMPAO SPECT. The count rate ratios in [sup 99m]Tc-ECD and [sup 99m]Tc-HMPAO SPECT were correlated well with each other, and the contrast of the [sup 99m]Tc-ECD SPECT image was equivalent or slightly higher as compared with [sup 99m]Tc-HMPAO. Therefore, [sup 99m]Tc-ECD SPECT study was considered to be useful for the evaluation of cerebral perfusion in dementia and aphasia. (author).

  13. 99mTc-ECD SPECT study in dementia and aphasia

    International Nuclear Information System (INIS)

    We studied clinical significance of 99mTc-L,L,-ethyl cysteine dimer (99mTc-ECD) SPECT study in dementia and aphasia, and compared it with 99mTc-HMPAO SPECT study. The subjects consisted of 13 patients, including 10 patients with dementia and 3 patients with aphasia. Hypoperfusion areas were detected in 5 out of 10 patients with dementia and 2 out of 3 patients with aphasia in 99mTc-ECD SPECT, and in 4 out of 10 patients with dementia and all of 3 patients with aphasia in 99mTc-HMPAO SPECT. The count rate ratios in 99mTc-ECD and 99mTc-HMPAO SPECT were correlated well with each other, and the contrast of the 99mTc-ECD SPECT image was equivalent or slightly higher as compared with 99mTc-HMPAO. Therefore, 99mTc-ECD SPECT study was considered to be useful for the evaluation of cerebral perfusion in dementia and aphasia. (author)

  14. Comparative microvascular exchange kinetics of [{sup 77}Br]bromide and {sup 99m}Tc-DTPA in humans

    Energy Technology Data Exchange (ETDEWEB)

    Cousins, Claire [Department of Clinical Radiology, Addenbrooke' s Hospital, Cambridge (United Kingdom); Skehan, Stephen J.; Rolph, Susan M.; Flaxman, Mary E.; Ballinger, James R.; Bird, Nicholas J.; Barber, Robert W.; Peters, Michael A. [Department of Nuclear Medicine, Addenbrooke' s Hospital, Cambridge (United Kingdom)

    2002-05-01

    The plasma clearance curves of small hydrophilic solutes comprise three exponentials, consistent with a three-compartmental distribution model. A previous comparison between inulin and diethylene triamine penta-acetic acid (DTPA) suggested that these three compartments are in series, the first being plasma and the second and third representing compartments within the extravascular space. Moreover, whilst the total distribution volumes of these two indicators were similar, the volume of the second compartment was higher for DTPA. The purpose of the current study was to investigate whether a solute smaller than DTPA, namely bromide, fits the hypothesis that the second space volume is an inverse function of the size of the solute. Two groups of subjects were studied: group A comprised eight patients undergoing routine diagnostic arteriography and group B, eight patients referred for routine measurement of glomerular filtration rate plus two normal volunteers. {sup 99m}Tc-DTPA and sodium [{sup 77}Br]bromide were intravenously administered simultaneously. In group A, frequent arterial samples were obtained up to 40 min after injection, and antecubital venous samples 30 s after each arterial sample. In group B, frequent venous samples were obtained up to 280 min after injection. Volume measurements based on bromide were corrected for erythrocyte bromide accumulation. In both subject groups, the normalised venous concentration ratio of bromide to DTPA, corrected for red cell bromide uptake, was significantly less than unity in the earliest blood samples, being 0.56 (SD 0.08) at 1 min, consistent with faster diffusion of bromide from plasma to interstitial fluid. Furthermore, the extraction fraction of bromide from plasma to interstitial fluid in the forearm was about 0.6, higher than that of DTPA (about 0.5) in spite of red cell bromide accumulation which equilibrated with plasma bromide within 20 s and resulted in a red cell to plasma concentration ratio of 0.51 (0

  15. Effect of plasmapheresis on the liver uptake of ApoB-lipoproteins labeled with technetium-99m

    International Nuclear Information System (INIS)

    To study liver low density lipoprotein (LDL)-receptor activity before and after plasmapheresis, [99mTc] very low density lipoprotein (VLDL) was used. Autologous VLDL was labeled, sterilized by filtration, and administered intravenously to patients under a gamma camera. The uptake of lipoproteins in the liver was measured by scintiscanning. Liver activity curves were generated for each patient. The liver activity in patients with the heterozygous form of familial hypercholesterolemia (FH) and in patients with symptomatic atherosclerosis (SA) without hereditary deficit of LDL receptors was reduced as compared to healthy people. Plasmapheresis enhanced the liver uptake of the 99mTc-labeled lipoproteins in atherosclerotic patients. Thus, labeled metabolites could presumably be of use in assessing the effect of plasmapheresis on liver function

  16. Radiobiologic effect of radiation emitted by Tc-99m as determined with a bioluminescent bacterial dosimetry system

    International Nuclear Information System (INIS)

    Previous work with light-emitting bacteria has shown that, after external irradiation, there is a decrease in bioluminescence and that the decrease is related to the absorbed dose and relative biological effectiveness (RBE). This project is designed to use these bacteria for internal dosimetry of Tc-99m and to compare such dosimetry with calculations of absorbed dose that uses standard methods. Sixty-millicurie and .02-mCi samples of Tc-99m were added to 1-mL water solutions containing approximately 1 million luminescent bacteria. Light emitted from these bacteria was measured at 30-minute intervals for 4 1/2 hours by means of a photomultiplier detection system. Bioluminescent bacterial survival curves were thus obtained

  17. {sup 99m}Tc reduces clonogenic survival after intracellular uptake in NIS positive cells in vitro more than {sup 131}I; {sup 99m}Tc reduziert nach intrazellulaerer Aufnahme in NIS-positiven Zellen in vitro das klonogene Ueberleben staerker als {sup 131}I

    Energy Technology Data Exchange (ETDEWEB)

    Wendisch, M.; Freudenberg, R.; Drechsel, J.; Runge, R.; Wunderlich, G.; Kotzerke, Joerg [Universitaetsklinikum Carl Gustav Carus Dresden - Technische Univ. Dresden (Germany). Klinik und Poliklinik fuer Nuklearmedizin

    2010-07-01

    In addition to gamma radiation of 140 keV {sup 99m}Tc emits during the transition to {sup 99}Tc electrons of low energy and tiny path-lengths. These Auger electrons cannot be utilized in diagnostic procedures. However, they were discussed frequently for therapeutic application. Hitherto proof of effect of the Auger electrons from {sup 99m}Tc is missing which is supplied now in an in vitro-system in comparison to beta-emitter {sup 131}I. The thyroid cell line PC CI3 (sodium iodide symporter (NIS)-positive) was incubated with {sup 131}I-sodium iodide ({sup 131}I) or {sup 99m}Tc-pertechnetate ({sup 99m}Tc) in presence or absence of perchlorate. For comparison the amount of radioactivity was adjusted to obtain the same dose from extracellular irradiation for both radionuclides. The colony forming assay detects the clonogenic cell survival as surviving fraction. In addition, intracellular radionuclide uptake was quantified. Dose effect curves were established for {sup 131}I and {sup 99m}Tc for variable extra- and intracellular distribution of the radioactivity. In presence of perchlorate no cellular uptake of radioactivity was detectable. Survival curves were largely comparable confirming the dosimetric calculations. In absence of perchlorate cellular radiotracer uptake varied from 1.39% ({sup 131}I) to 1.90% ({sup 99m}Tc). Effects on survival were twice for the beta-emitter and ten-fold higher for {sup 99m}Tc. Intracellular uptake of {sup 131}I and {sup 99m}Tc increases DNA-damage compared to strict extracellular radiotracer distribution which was demonstrated by means of colony forming assay. Increasing radiotoxicity from intracellular {sup 99m}Tc is explained most likely by increased dose deposition in cellular structures due to Auger- und conversion-electrons of low range and high local energy deposition. (orig.)

  18. Hydroxamamide as a chelating moiety for the preparation of 99mTc-radiopharmaceuticals. Pt. 3: Characterization of various 99mTc-hydroxamamides

    International Nuclear Information System (INIS)

    Five hydroxamamide (Ham) derivatives with various nonpolar substituents and different lipophilicities have been selected as ligands for preparation of 99mTc-complexes. Analysis via high performance liquid chromatography (HPLC) resolved the 99mTc-complexes of each ligand into two radioactive components, which are considered as isomers. The stability of both components was studied at pH 5 and 7, showing good stability at pH 7, but some transition between the components at pH 5. Use of mixture of two ligands for complexation showed not only the complexes of the separate ligands, but also complexes containing both ligands. These results indicate that hydroxamamides very likely form complexes composed of Ham and 99mTc in a 2:1 molar ratio. This may also explain the stability of the 99mTc-Ham complexes in spite of the bidentate character of a Ham ligand. (author)

  19. Guarana (Paullinia cupana) effect on radiolabelling of erythrocytes with Technetium-99m; Efeito do guarana (Paulinnia cupana) na marcacao de hemaceas com tecnecio-99m

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Joelma F.; Braga, Ana Cristina S.; Avila, Antonio Sergio R.; Gutfilen, Bianca [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Biologia; Bernardo-Filho, Mario [Instituto Nacional do Cancer, Rio de Janeiro, RJ (Brazil)

    1996-07-01

    The labelling of red blood cells (RBC) with technetium-99m (Tc-99m) have been used for many studies in nuclear medicine. This labeling procedure depends on a reducing agent and stannous chloride is normally used for this purpose. Many factors, including drug therapy, can affect the biodistribution of radiopharmaceuticals, such factors many lead to poor organ visualization, requirement to repeat the examination procedure or even misdiagnosis. Guarana (Paullinia cupana) is commonly used in popular medicine. Here, we investigate if guarana is capable to alter the labeling of RBC with Tc-99m and to modify the radioactivity binding to plasma proteins and cellular constituents from insoluble and soluble fractions. The results were obtained with blood samples from Wistar rats with heparine and incubated with different guarana solutions. The percentage of radioactivity (% rad) from BC and P were calculated. The distribution of radioactivity in P and BC showed that the uptake of Tc-99m decreased significantly in all concentrations. (author)

  20. Comparative study of 188Re( V )-DMSA and 99mTc ( V )-DMSA in tumor model%188Re(V)-DMSA与99mTc(V)-DMSA在肿瘤模型体内的对比研究

    Institute of Scientific and Technical Information of China (English)

    孙逊; 安锐

    2004-01-01

    Objective To compare the biodistribution and imaging characteristics of 188Re(V)-DMSA and 99mTc(V)-DMSA in tumor model, and to discuss the possibility of treating tumors with 188Re(V)-DMSA. Methods The solid neoplasm bearing mice (Ehrlich carcinoma bearing mice) models underwent biodistribution study and static whole body planar imaging after injection of 188Re(V)-DMSA and 99mTc(V)-DMSA respectively. When the mice were sacrificed at different time after the injection, the tumor, blood and contralateral normai muscles were removed, weighted and the radioactivity was measured. Then the radioactivity ratios of target (tumor)-to-blood (T/B),target-to-non targeted (contralateral limbs or muscles) (T/NT) were calculated. ROIs were drawn and T/NT were calculated in planar imagines. Results Two radiopharmaceuticals were mainly concentrated in bone and kidney, and the uptake ratios in tumor were high too.The half-clearance times of these two radiopharmaceuticals in blood were both less than 1h. The greatest T/NT ratio of 99mTc group was higher than 188Re group in planar imagings, but the highest T/B, T/NT ratios of these two radiopharmaceuticals in biodistribution study had no significant difference and were all above 3.0. Conclusion The biodistribution characteristics of 188Re(V)-DMSA and 99mTc( V)-DMSA were similar. 188Re(V)-DMSA has good applied foreground in treating tumors and their metastases.%目的比较188Re(V)-DMSA(五价188Re-二巯基丁二酸钠)和99mTc(V)-DMSA在肿瘤模型体内生物分布与显像的特点,探讨188Re(V)-DMSA用于肿瘤治疗的可能性.方法用188Re(V)-DMSA和99mTc(V)-DMSA对实验性实体肿瘤(小鼠艾氏腹水癌)模型进行生物学分布实验和全身平面显像,并通过脏器克组织百分摄取率(%ID/g)测定法和感兴趣区(ROI)技术进行定量分析,计算各时点两种放射性药物的靶/血、靶/非靶比值.结果两种放射性药物均主要浓聚于骨骼和肾脏,肿瘤组织也有较高的摄

  1. Evaluation of adenosine preconditioning with 99mTc-His10-annexin V in a porcine model of myocardium ischemia and reperfusion injury: preliminary study

    International Nuclear Information System (INIS)

    Purpose: The goal of this study was to evaluate the feasibility of 99mTc-His10-annexin V for the detection of acute myocardial cell death and to assess the effect of adenosine preconditioning in a porcine model of myocardium ischemia and reperfusion injury (RI). Materials and Methods: 99mTc-His10-annexin V was prepared by one-step direct labeling, and RCP and radiostability were tested. The binding of 99mTc-His10-annexin V to apoptosis was validated in vitro using camptothecin-induced Jurkat cells. In vivo biodistribution was determined in mice by the dissection method. Ischemia of 20-30 min was induced by balloon occlusion of the epicardial coronary artery of the porcine model (n=14). Adenosine was infused intravenously in six pigs before coronary occlusion. 99mTc-His10-annexin V (n=12) was injected intravenously at 1 h after reperfusion. SPECT/CT was acquired at 3 h postinjection. Myocardial perfusion imaging (MPI) with 99mTc-MIBI was also performed 1 day after His10-annexin V imaging. Cardiac tissues were analyzed postmortem using hematoxylin-and-eosin and TUNEL staining. Caspase-3 activity was measured to confirm the presence of apoptosis. Results: 99mTc-His10-annexin V had a RCP >98% and high stability 2 h after radiolabeling; it could bind to apoptotic cells with high affinity. Biodistribution of 99mTc-His10-annexin V showed a predominant uptake in the kidney and relatively low uptake in the myocardium, liver and gastrointestinal tract; rapid clearance from blood and kidney was observed. In the untreated group, intense uptake of His10-annexin V was visualized in the defect which was shown in MPI, whereas in the adenosine group a mild uptake of 99mTc-His10-annexin was found in the risk area which showed no defects in the 99mTc-MIBI image. TUNEL staining and activated caspase-3 confirmed the ongoing apoptosis in RI. Adenosine preconditioning significantly diminished the level of apoptosis. Uptake of His10-annexin V in RI correlated with TUNEL-positive nuclei

  2. Renal vascular reverse could stratify angiopathy in diabetic patients using baseline an acetazolamide Tc99mMAG-3 renography

    International Nuclear Information System (INIS)

    Purpose: The aim of this study was to determine whether or not Tc99mMAG-3 renography can stratify the severity of renal parenchymal damage related to angiopathy in patients with normal, hypertension and diabetes with hypertension by combining baseline with Acetazolamide (ACZ), an inhibitor of carbonic anhydrase, renography. Methods: We obtained the informed consent of 30 patients to participate in this study of baseline and ACZ renography. There were divided into three groups of 10 each as follows: normal (NR), essential hypertension (EH) and Type 2 diabetes with hypertension (DM). We performed baseline renography for 20 min after an injection of 370 MBq Tc99mMAG-3, followed by ACZ renography performed in the same manner 10 min after a 1g injection ACZ. Quantitative analyses included tubular extraction rate (TER), effective renal plasma flow (ERPF) and time to the maximum activity of the renal curve (Tmax) determined using the deconvolution method applied to the time activity curve of Tc99mMAG-3 in the kidneys. The percent change in TER (ERPF and Tmax) between the baseline and ACZ renography was obtained in the following manner: %TER (%ERPF and %Tmax) = [ACZ TER (ERPF and Tmax) - baseline TER (ERPF and Tmax)] x 100/baseline TER (ERPF and Tmax) Corrected %TER (%ERPF and % Tmax) was then obtained by subtracting the variation in %TER (%ERPF and % Tmax) between baseline and sequential renograms derived from 10 control patients (CR group) injected with distilled water, from those injected with ACZ. Results: These parameters did not significantly differ between the Nr and Eh groups, but the differences between the Dm and Nr groups and between the Dm and Eh groups were significant (p<0.01). Conclusion: Combined baseline and ACZ Tc99mMAG-3 renography can evaluate renal vascular reverse, a loss of which could represent microangiopathy in diabetic patients (au)

  3. Technetium-99m tetrofosmin for parathyroid scintigraphy: a direct comparison with {sup 99m}Tc-MIBI, {sup 201}Tl, MRI and US

    Energy Technology Data Exchange (ETDEWEB)

    Wakamatsu, Hideyuki [Dept. of Radiology, Noguchi Thyroid Clinic and Hospital Foundation, Oita (Japan); Noguchi, Shiro; Yamashita, Hiroyuki [Dept. of Surgery, Noguchi Thyroid Clinic and Hospital Foundation, Oita (Japan); Yamashita, Hiroto [Dept. of Pathology, Noguchi Thyroid Clinic and Hospital Foundation, Oita (Japan); Tamura, Shozo; Jinnouchi, Seishi; Nagamachi, Shigeki; Futami, Shigemi [Dept. of Radiology, Miyazaki Medical College, Miyazaki (Japan)

    2001-12-01

    The aim of this study was to evaluate the efficacy and role of technetium-99m tetrofosmin for the detection of abnormal parathyroid glands to be referred for surgical treatment. Twenty-eight consecutive patients, including 25 primary and 3 secondary cases of hyperparathyroidism, were evaluated. {sup 99m}Tc-tetrofosmin/{sup 99m}Tc-pertechnetate subtraction scintigraphy (TF/Tc) was performed on all patients, and the results were directly compared with those of {sup 99m}Tc-methoxyisobutylisonitrile (MIBI)/{sup 99m}Tc-pertechnetate subtraction scintigraphy (MIBI/Tc), {sup 201}Tl/{sup 99m}Tc-pertechnetate subtraction scintigraphy (Tl/Tc), magnetic resonance imaging (MRI) and ultrasonography (US). In cases of single-gland disease, the sensitivities of TF/Tc, MIBI/Tc, Tl/Tc, MRI and US were 63.2%, 68.4%, 57.9%, 55.6% and 63.2%, respectively. In cases of multi-gland disease, the sensitivities of TF/Tc, MIBI/Tc, Tl/Tc, MRI and US were 41.7%, 41.7%, 37.5%, 58.3% and 54.2%, respectively. In cases of parathyroid adenoma, the sensitivities of TF/Tc, MIBI/Tc, Tl/Tc, MRI and US were 68.8%, 75.0%, 68.8%, 62.5% and 75.0%, respectively. In cases of parathyroid hyperplasia, the sensitivities of TF/Tc, MIBI/Tc, Tl/Tc, MRI and US were 40.7%, 40.7%, 33.3%, 53.8% and 48.1%, respectively. It is concluded that, for the detection of abnormal parathyroid glands, {sup 99m}Tc-tetrofosmin is as useful as {sup 99m}Tc-MIBI and is more useful than {sup 201}Tl. (orig.)

  4. Preparation and In Vitro Evaluation of Tc-99m Labeled Octreotide%99mTc-octreotide的标记及受体分析

    Institute of Scientific and Technical Information of China (English)

    叶千春; 吴翼伟; 范我; 江一民; 章斌

    2003-01-01

    目的探讨直接法标记99mTc-octreotide的方法学及其与大鼠脑皮质细胞的亲和力.方法用直接法标记99mTc-octreotide,探讨其最佳标记条件;并以99mTc-octreotide作为配基,检测大鼠脑皮质细胞与其结合的平衡解离常数.结果99mTc-octreotide标记率为53%~57%,经Sephadex G-10纯化后放化纯大于95%;受体分析显示大鼠脑皮质细胞与99mTc-octreotide保持了较高的亲和力(Kd=10.4nM).结论本标记方法是可行的,99mTc-octreotide有希望作为具有临床实用价值的生长抑素受体显像剂.

  5. Characterization of technetium-99m-L,L-ECD for brain perfusion imaging, Part 1: Pharmacology of technetium-99m ECD in nonhuman primates

    Energy Technology Data Exchange (ETDEWEB)

    Walovitch, R.C.; Hill, T.C.; Garrity, S.T.; Cheesman, E.H.; Burgess, B.A.; O' Leary, D.H.; Watson, A.D.; Ganey, M.V.; Morgan, R.A.; Williams, S.J. (E.I. Du Pont de Nemours Co., Inc., No. Billerica, MA (USA))

    1989-11-01

    Technetium-99m ethyl cysteinate dimer (({sup 99m}Tc)ECD) is a neutral, lipophilic complex which rapidly crosses the blood-brain barrier. Brain retention and tissue metabolism of ({sup 99m}Tc)ECD is dependent upon the stereochemical configuration of the complex. While both L,L and D,D enantiomers are extracted by the brain, only the L,L but not the D,D form, is metabolized and retained in the monkey brain (4.7% injected dose initially, T 1/2 greater than 24 hr). Dynamic single photon emission computed tomography imaging studies in one monkey indicates {sup 99m}Tc-L,L-ECD to be distributed in a pattern consistent with regional cerebral blood flow for up to 16 hr postinjection. Dual-labeled {sup 99m}Tc-L,L-ECD and ({sup 14}C)iodoantipyrine autoradiography studies performed 1 hr after administration show cortical gray to white matter ratios of both isotopes to be equivalent (approximately 4-5:1). These data suggest that {sup 99m}Tc-L,L-ECD will be useful for the scintigraphic assessment of cerebral perfusion in humans.

  6. labeling of some organic compounds of expected biological activity with Tc-99m eluted from a chromatographic column packed with zirconium molybdate containing mo-99

    International Nuclear Information System (INIS)

    using different analytical techniques like elemental analysis, IR, 1H-NMR and mass spectroscopy.The synthesized iminodiacetic acid derivatives (NIDA, DMIDA and BPIDA) were labeled with 99mTc and the optimum conditions used to prepare the 99mTc-NIDA, 99mTc-DMIDA and 99mTc-BPIDA can be summarized as follow:NIDA: 30 mg NIDA + 0.3 mg SnCl2.2H2O at ph 6 after 15 min, it gives 94. 2±2% labeling yield with 99mTc showing stability up to 6 h.DMIDA: 3 mg DMIDA + 30μg SnCl2.2H2O at ph 6 after 15 min, it gives 93.1±2% labeling yield with 99mTc showing stability up to 6 h.BPIDA: 2 mg BPIDA in the presence of 20μg SnCl2.2H2O at ph 6 after 15 min, it gives 85.4 ±3% labeling yield with 99mTc showing stability up to 6 h.Biodistribution studies of 99mTc-NIDA, 99mTc-DMIDA and 99mTc-BPIDA in mice showed that most of the injected activity was transported to the liver and cleared into the gallbladder and intestine with a minimal uptake in the other organs, a high degree of liver uptake and fast clearance where the activity in the liver was 19.23%, 25.17% and 18.63% after 10 min post injection, respectively, and cleared rapidly till reaching 3.31%, 7.21% and 5.2% after 3 h, respectively. The accumulation of the activity in the stomach was low, 0.47%, 0.28% and 1.07% after 60 min post injection indicating that 99mTc-NIDA, 99mTc-DMIDA and 99mTc-BPIDA complex remains stable without any decomposition inside the body.

  7. Synthesis, characterization, biodistribution and scintigraphy of 99mTc-paclitaxel. A potential tracer of paclitaxel

    International Nuclear Information System (INIS)

    99mTc-paclitaxel was synthesized by using sodium borohydride as a reducing agent. Greater than 95 % labelling efficiency was achieved. Radiochemical purity of the synthesized 99mTc-paclitaxel was validated by thin layer chromatography (TLC) scanner and high performance liquid chromatography (HPLC). 99mTc-paclitaxel passed in vitro stability tests. Biodistribution and scintigraphy studies were performed in Sprague-Dawley rats. The biodistribution study results of 99mTc-paclitaxel were related mainly to the metabolism and excretion routes followed by the parental drug, paclitaxel. Apart from that, biodistribution of 99mTc-paclitaxel was altered after pre-treatment with cold paclitaxel. Hence, 99mTc-paclitaxel may be used as a tracer for paclitaxel. (author)

  8. Organ distribution of sup(99m)Tc-Sn tetracycline antibiotics in rats

    International Nuclear Information System (INIS)

    The organ distribution of [sup(99m)Tc-Sn]tetracycline hydrochloride, [sup(99m)Tc-Sn]oxytetracycline hydrochloride and [sup(99m)Tc-Sn]rolitetracycline nitrate was studied in rats. It was shown that these preparations have a very similar organ distribution and are predominantly deposited in the kidneys. The maximum renal radioactivity level was found 2 to 4 hours after intravenous administration of the preparation, this, for [sup(99m)Tc-Sn]tetracycline hydrochloride 18.60%; for [sup(99m)Tc-Sn]oxytetracycline hydrochloride 17.09.=.; for [sup(99m)Tc-Sn]rolitetracycline nitrate 20.12%. The activity levels in the muscle, liver, heart, brain, lungs, stomach and spleen are minimal. (author)

  9. 99mTc-MAMA-HA与99mTcN-MAMA-HA的制备及生物性能的比较%THE PREPARATION AND BIOLOGICAL PROPERTIES OF 99mTc-MAMA-HA AND 99mTcN-MAMA-HA

    Institute of Scientific and Technical Information of China (English)

    吴战宏; 王学斌; 李至敏

    2002-01-01

    制备了2种脂肪酸标记物99mTc-MAMA-HA和99mTcN-MAMA-HA,改进了标记方法,放化纯均大于90%;对标记物进行了小鼠的生物分布实验,结果表明二者都有一定的心肌摄取,99mTc-MAMA-HA的心肌摄取要好于99mTcN-MAMA-HA,而后者的心肌滞留要好于前者,这说明99mTcN的引入明显改变了99mTc-MAMA-HA的生物分布.

  10. Assessment of alveolar epithelial permeability in progressive systemic sclerosis (PSS) using 99mTc-DTPA (diethylene triamine penta acetate) aerosol inhalation

    International Nuclear Information System (INIS)

    To evaluate alveolar epithelial damage in PSS, we studied pulmonary epithelial permeability by measuring the clearance of inhaled 99mTc-DTPA aerosol and performing thin slice CT scan, pulmonary function tests and right heart catheterization in 28 patients with PSS. The 99mTc-DTPA clearance rate (kep value) in PSS was greater than in 11 non-smoking normal subjects (18.2±7.63x10-3/min vs. 9.12±0.77x10-3/min, p2. In contrast, the kep value showed significant correlations with %DLco (diffusing capacity for carbon monoxide), extent of interstitial lesions evaluated by CT scan (CT score), and mean pulmonary artery pressure. On the other hand, the kep value was high in some patients with normal CT scan and normal %DLco. These findings indicate that pulmonary interstitial lesions in PSS are accompanied by alveolar epithelial damage, and that the clearance of 99mTc-DTPA may be an early predictor of interstitial change. (author)

  11. Is Brown Adipose Tissue Visualization Reliable on 99mTc-Methoxyisobutylisonitrile Diagnostic SPECT Scintigraphy?

    OpenAIRE

    Haghighatafshar, Mahdi; Farhoudi, Farinaz

    2016-01-01

    Abstract The 99mTc-MIBI has been used with great value as a diagnostic technique in patients with primary hyperparathyroidism. False-positive scans may occur due to misinterpretation of the physiologic distribution of the 99mTc-MIBI. Reviewing consecutive SPECT scans, we evaluated this possibility and assessed how frequently brown adipose tissue (BAT) is seen on 99mTc-MIBI scintigraphy. Here, we retrospectively reviewed scans of consecutive patients who were evaluated for parathyroid adenomas...

  12. Physicochemical and biological study of a renal scintigraphy agent: the DMSA - 99mTc complex

    International Nuclear Information System (INIS)

    This research thesis deals with the study of the dimercaptosuccinic acid (DMSA) marked with 99mTc, a recently developed scintigraphy agent used for the kidney isotopic exploration. The author notably studied the relationships between the physicochemical properties of solutions of dimercaptosuccinic acid marked with 99mTc and the biological distribution of 99mTc in order to reach a better understanding of the biological mechanism which results in technetium fixation to the kidney

  13. Diagnosis of orbital cavernous hemangioma with Tc-99m RBC SPECT.

    Science.gov (United States)

    Ki, W W; Shin, J W; Won, K S; Ryu, J S; Yang, S O; Lee, H K; Kim, Y J

    1997-08-01

    The authors report two cases of orbital cavernous hemangioma diagnosed by Tc-99m RBC SPECT. Tc-99m RBC SPECT showed a typical scintigraphic pattern commonly seen in hepatic hemangioma in which there is intense focally increased uptake on delayed SPECT images. Tc-99m RBC SPECT in orbital cavernous hemangioma may be as useful a diagnostic modality as in hepatic hemangioma. PMID:9262901

  14. Technetium-99m labeling anti-amastigote polyclonal antibodies of Leishmania amazonensis

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, J.G.V.C.; Toledo, V.P.C.P.; Guimaraes, T.M.P.D.; Bernardo-Filho, M.; Simal, C.J.R.; Mota, L.G.; Diniz, S.O.F.; Cardoso, V.N. E-mail: cardosov@farmacia.ufmg.br

    2002-05-01

    Anti-amastigote polyclonal antibody (IgG) was incubated with solutions of stannous chloride and sodium borohidride. After that, 3.7 MBq of technetium-99m ({sup 99m}Tc) was added. A labeling yield of the antibody about 84% was obtained. After filtration of {sup 99m}Tc-IgG, the radiochemical purity increased from 84 to 95%. The labeling of IgG with {sup 99m}Tc did not modify the immunoreactivity of the antibody, since it was able to identify in vitro and in vivo the specific antigen of Leishmania amazonensis.

  15. A new technology of technetium-99m production for medicine imaging

    Energy Technology Data Exchange (ETDEWEB)

    Dovbnya, A.N.; Dikiy, N.P.; Medvedyeva, Ye.P.; Tur, Yu.D.; Uvarov, U.L. [National Science Center Kharkov Inst. of Physics and Technology, Kharkov (Ukraine)

    1998-07-01

    Technetium-99m provides now up to 90% of medical analysis using gamma-scintigraphy method. The parent isotope {sup 99} Mo is produced mainly in fission reactors, that is rather technically complicated and ecologically unwonted. The report contains a short description of new approach to {sup 99m} Tc production problem using irradiation of Mo-target with mixed e, n, {gamma}-radiation of electron accelerator and extraction of {sup 99m} Tc by electrolysis. Medical test of {sup 99m} Tc based radiopharmaceutical thus produced their high isotope purity. (author)

  16. Usefulness of per-rectal portal scintigraphy with Tc-99m pertechnetate for galactosemia in infants

    Energy Technology Data Exchange (ETDEWEB)

    Shiomi, Susumu; Sasaki, Nobumitsu; Ikeoka, Naoko; Kuroki, Tetsuo; Okano, Yoshiyuki; Kawabe, Joji; Ochi, Hironobu [Osaka City Univ. (Japan). Medical School

    1998-12-01

    Galactosemia discovered by newborn screening is rarely caused by enzyme deficiency. It has recently been reported that among patients without enzyme deficiency portosystemic shunting may be a cause of galactosemia in some patients. We did per-rectal portal scintigraphy in patients with such galactosemia detected during screening of newborns to examine the usefulness of this method for the diagnosis of portosystemic shunts via the inferior mesenteric vein. The subjects were eight neonates with galactosemia without enzyme deficiency detected during screening. A solution containing technetium-99m pertechnetate was instilled into the rectum, and serial scintigrams were taken while radioactivity curves for the liver and heart were recorded sequentially. The per-rectal portal shunt index was determined by calculating the ratio for counts of the liver to counts for the heart integrated for 24 seconds immediately after the appearance of the liver time-activity curve. A portosystemic shunt was detected in both of the patients with a shunt index of 30% or more, but not in the six patients with a shunt index less than 30%. The blood galactose levels of these six patients later entered the reference range. This method is noninvasive and there is little exposure to the radionuclide. It seemed to be useful for the diagnosis of portosystemic shunt in newborns with galactosemia without enzyme deficiency. (author)

  17. Kinetic sensitivity of a receptor-binding radiopharmaceutical: Technetium-99m galactosyl-neoglycoalbumin

    International Nuclear Information System (INIS)

    Kinetic sensitivity is the ability of a physiochemical parameter to alter the time-activity curve of a radiotracer. The kinetic sensitivity of liver and blood time-activity data resulting from a single bolus injection of [99mTc]galactosyl-neoglycoalbumin [( Tc]NGA) into healthy pigs was examined. Three parameters, hepatic plasma flow scaled as flow per plasma volume, ligand-receptor affinity, and total receptor concentration, were tested using [Tc]NGA injections of various molar doses and affinities. Simultaneous measurements of plasma volume (iodine-125 human serum albumin dilution), and hepatic plasma flow (indocyanine green extraction) were performed during 12 [Tc]NGA studies. Paired data sets demonstrated differences (P(chi v2) less than 0.01) in liver and blood time-activity curves in response to changes in each of the tested parameters. We conclude that the [Tc]NGA radiopharmacokinetic system is therefore sensitive to hepatic plasma flow, ligand-receptor affinity, and receptor concentration. In vivo demonstration of kinetic sensitivity permits delineation of the physiologic parameters that determine the biodistribution of a radiopharmaceutical. This delineation is a prerequisite to a valid analytic assessment of receptor biochemistry via kinetic modeling

  18. Lyophilized kits of diamino dithiol compounds for labelling with 99m-technetium. Pharmacokinetics studies and distribution compartmental models of the related complexes

    International Nuclear Information System (INIS)

    The present work reflects the clinical interest for labelling diamino dithiol compounds with technetium-99m. Both chosen compounds, L,L-Ethylene dicysteine (L,L-EC) and L,L-Ethylene dicysteine diethyl esther (L,L-ECD) were obtained with relative good yield and characterized by IR and NMR. The study of labelling conditions with technetium-99m showed the influence of the type and mass of reducing agent as well as the pH on the formation of complexes with desired biological characteristics. Radiochemical purity was determined by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Lyophilised kits of L,L-EC and L,L-ECD for labelling with 99mTc were obtained, with stability superior to 120 days, when stored under refrigeration, enabling the kits marketing. The ideal formulation of the kits as well as the use of liquid nitrogen in the freezing process, determined the lyophilization success. Distribution biological studies of the 99mTc complexes were performed on mice by invasive method and on bigger animals by scintigraphic evaluation. Biological distribution studies of the complex 99mTc-L,L-EC showed fast blood clearance, with the elimination of about 90% of the administered dose after 60 minutes, almost exclusively by the urinary system. The biological distribution results were adjusted to a three compartmental distribution model, as expected for a radiopharmaceutical designed to renal dynamic studies, with tubular elimination. The complex interaction with renal tubular receptors is related with structural characteristics of the compound, more specifically with the presence and location of polar groups. In comparison with 99mTc-L,L-EC, biological studies of the complex 99mTc -L,L-ECD showed different distribution aspects, despite some structural similarities. The presence of ethyl groups confers to the complex neutrality and lipophilicity. It cross the intact blood brain barrier and is retained in the brain for enough period, permitting

  19. Effective detection of the tumors causing osteomalacia using [Tc-99m]-HYNIC-octreotide (99mTc-HYNIC-TOC) whole body scan

    Energy Technology Data Exchange (ETDEWEB)

    Jing, Hongli, E-mail: annsmile1976@sina.com [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Li, Fang, E-mail: lifang@pumch.cn [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Zhuang, Hongming, E-mail: zhuang@email.chop.edu [Department of Radiology, The Children' s Hospital of Philadelphia, University of Pennsylvania Perelman School of Medicine, 34th and Civic Ctr Blvd, Philadelphia, PA 19104 (United States); Wang, Zhenghua, E-mail: ccq1214@yahoo.com.cn [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Tian, Jian, E-mail: tianjian4809@hotmail.com [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Xing, Xiaoping, E-mail: xingxp@126.com [Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Jin, Jin, E-mail: jinjin9010@126.com [Department of Orthopedic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Zhong, Dingrong, E-mail: ZhongDR@pumch.cn [Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China); Zhang, Jingjing, E-mail: zhangjingjingtag@163.com [Department of Nuclear Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730 (China)

    2013-11-01

    Purpose: Tumor-induced osteomalacia (TIO) is an endocrine disorder caused by tumors producing excessive fibroblast growth factor-23 (FGF-23). The causative tumors are generally small, slow-growing benign mesenchymal tumors. The only cure of the disease depends on resection of the tumors, which are extremely difficult to localize due to their small sizes and rare locations. Since these tumors are known to express somatostatin receptors, this research was undertaken to evaluate efficacy of [Tc-99m]-HYNIC-octreotide (99mTc-HYNIC-TOC) whole body imaging in this clinical setting Methods: Images of 99mTc-HYNIC-TOC scans and clinical chart from 183 patients with hypophosphatemia and clinically suspected TIO were retrospectively reviewed. The scan findings were compared to the results of histopathological examinations and clinical follow-ups. Results: Among 183 patients, 72 were confirmed to have TIO while 103 patients were found to have other causes of hypophosphatemia. The possibility of TIO could not be either diagnosed or excluded in the remaining 8 patients. For analytical purposes, these 8 patients who could neither be diagnosed nor excluded as having TIO were regarded as having the disease, bringing the total of TIO patients to 80. The 99mTc-HYNIC-TOC scan identified 69 tumors in 80 patients with TIO, which rendered a sensitivity of 86.3% (69/80). 99mTc-HYNIC-TOC scintigraphy excluded 102 patients without TIO with a specificity of 99.1% (102/103). The overall accuracy of 99mTc-HYNIC-TOC whole body scan in the localization of tumors responsible for osteomalacia is 93.4% (171/183). Conclusions: Whole body 99mTc-HYNIC-TOC imaging is effective in the localization of occult tumors causing TIO.

  20. Effective detection of the tumors causing osteomalacia using [Tc-99m]-HYNIC-octreotide (99mTc-HYNIC-TOC) whole body scan

    International Nuclear Information System (INIS)

    Purpose: Tumor-induced osteomalacia (TIO) is an endocrine disorder caused by tumors producing excessive fibroblast growth factor-23 (FGF-23). The causative tumors are generally small, slow-growing benign mesenchymal tumors. The only cure of the disease depends on resection of the tumors, which are extremely difficult to localize due to their small sizes and rare locations. Since these tumors are known to express somatostatin receptors, this research was undertaken to evaluate efficacy of [Tc-99m]-HYNIC-octreotide (99mTc-HYNIC-TOC) whole body imaging in this clinical setting Methods: Images of 99mTc-HYNIC-TOC scans and clinical chart from 183 patients with hypophosphatemia and clinically suspected TIO were retrospectively reviewed. The scan findings were compared to the results of histopathological examinations and clinical follow-ups. Results: Among 183 patients, 72 were confirmed to have TIO while 103 patients were found to have other causes of hypophosphatemia. The possibility of TIO could not be either diagnosed or excluded in the remaining 8 patients. For analytical purposes, these 8 patients who could neither be diagnosed nor excluded as having TIO were regarded as having the disease, bringing the total of TIO patients to 80. The 99mTc-HYNIC-TOC scan identified 69 tumors in 80 patients with TIO, which rendered a sensitivity of 86.3% (69/80). 99mTc-HYNIC-TOC scintigraphy excluded 102 patients without TIO with a specificity of 99.1% (102/103). The overall accuracy of 99mTc-HYNIC-TOC whole body scan in the localization of tumors responsible for osteomalacia is 93.4% (171/183). Conclusions: Whole body 99mTc-HYNIC-TOC imaging is effective in the localization of occult tumors causing TIO

  1. Development of Tc99m-Saccharic Acid for Heart Imaging

    International Nuclear Information System (INIS)

    Cardiovascular disease especially the coronary heart disease (CHD) is the leading cause of death worldwide. Coronary heart disease is a common term for the buildup of plaque in the heart coronary arteries that could block the blood supply to the myocardial and this could lead to heart attack. An estimated 17 million people died from cardiovascular disease in 2008 representing 30% of all global death. In United Kingdom, coronary heart disease killed as much as 82,000 people each year. Hence, early detection of the coronary heart disease is very important in reducing the mortality among the world population. One of the most sensitive detection methods is by radioimaging using Technetium-99m radiopharmaceuticals. Several different radio imaging agents such as Tc99m radiopharmaceutical were developed as radiagnostic agent in determining the CHD especially in identifying the blockage of the coronary artery of the heart muscle. Despite the success of Tc99m-sestamibi and Tc99m-tetrofosmin as effective agents for myocardial perfusion study, the search for other Tc99m heart imaging agents has never been interrupted. This report described the formulation of the Tc99m-saccharic acid radiopharmaceutical kit, radiolabelling of the kit, radiochemical purity evaluation of the Tc99m labeled saccharaic acid, and animal study involving radio imaging using gamma camera. The animal are then sacrificed and the biological distribution of the Tc99m-saccharic acid in-vivo was determined. Comparative study was also conducted using commercially available Tc99m-tetrafosmin, a CHD radiopharmaceutical kit. The Tc99m-saccharic acid developed gave a very high labeling efficiency of >92% with Tc99m and good uptake in the heart muscle. The saccharic acid kit developed was also found to be comparable in quality to the commercially available Tc99-tetrafosmin kit. (author)

  2. Effects of lung volume on clearance of solutes from the air spaces of lungs

    International Nuclear Information System (INIS)

    Several investigators have shown that the clearance rate of aerosolized 99mTc-labeled diethylenetriamine pentaacetate (DTPA, mol wt = 492, radius = 0.6 nm) from the air spaces of the lungs of humans and experimental animals increases with lung volume. To further investigate this phenomenon we performed a compartmental analysis of the 2-h clearance of DTPA from the lungs of anesthetized sheep using a new method to more accurately correct for the effects of DTPA recirculation. This analysis showed that the DTPA clearance in eight sheep ventilated with zero end-expired pressure was best described by a one-compartment model with a clearance rate of 0.42 +/- 0.15%/min. Ventilating eight sheep with an end-expired pressure of 10 cmH2O throughout the study increased the end-expired volume 0.4 +/- 0.1 liter BTPS and created a clearance curve that was best described by a two-compartment model. In these sheep 56 +/- 16% of the DTPA cleared from the lungs at a rate of 7.9 +/- 2.9%/min. The remainder cleared at a rate similar to that measured in the sheep ventilated with zero end-expired pressure (0.35 +/- 0.18%/min). Additional control and lung inflation experiments were performed using /sup 99m/Tc-labeled human serum albumin (mol wt = 66,000, radius = 3.6 nm). In six control sheep ventilated with zero end-expired pressure the albumin clearance was best described by a one-compartment model with a clearance rate of 0.06 +/- 0.02%/min. The clearance rate in six sheep with increased lung volume was slightly larger (0.09 +/- 0.02, P less than 0.05) but was well described by a one-compartment model

  3. The biological characterization of {sup 99m}Tc-BnAO-NI as a SPECT probe for imaging hypoxia in a sarcoma-bearing mouse model

    Energy Technology Data Exchange (ETDEWEB)

    Hsia, Chien-Chung [Institute of Biomedical imaging and Radiological Sciences, National Yang-Ming University, Taiwan (China); Institute of Nuclear Energy Research, Taiwan (China); Huang, Fu-Lei [Institute of Nuclear Energy Research, Taiwan (China); Hung, Guang-Uei [Department of Nuclear Medicine, Chang-Bing Show Chwan Hospital, Taiwan (China); Shen, Lie-Hang [Institute of Nuclear Energy Research, Taiwan (China); Chen, Chuan-Lin, E-mail: clchen2@ym.edu.t [Institute of Biomedical imaging and Radiological Sciences, National Yang-Ming University, Taiwan (China); Wang, Hsin-Ell, E-mail: hewang@ym.edu.t [Institute of Biomedical imaging and Radiological Sciences, National Yang-Ming University, Taiwan (China)

    2011-04-15

    Objectives: Tumor growth beyond the region where vascular oxygen can reach creates a hypoxic domain. In this study, BnAO, a ligand that had been labeled with {sup 99m}Tc-pertechnetate for hypoxia imaging, was conjugated with 2-nitroimidazole to give 3,3,10,10-tetramethyl-1-(2-nitro-1H-imidazo-1-y1)-4,9-diazadodecane-2,11- dionedioxime (BnAO-NI) as a potential ligand for hypoxia detection. Pentoxifylline is a peripheral vasodilator and has been used as a radiosensitizer in tumor radiotherapy. {sup 99m}Tc-BnAO-NI/SPECT was applied to noninvasively assess the pharmacological effect of pentoxifylline in reducing tumor hypoxia in vivo. Methods: BnAO-NI was synthesized and formulated with methylene diphosphonate (MDP), stannous chloride and carbonate buffer to afford kits. After mixing with {sup 99m}Tc-pertechnetate, {sup 99m}Tc-BnAO-NI injection can be readily prepared. The partition coefficient, radiochemical purity and in vitro stability were determined. Cellular uptake of radiotracers in KHT cells under hypoxia was conducted in a CO{sub 2} incubator at 37 {sup o}C under hypoxia or normoxia. A biodistribution study after intravenous injection of {sup 99m}Tc-BnAO-NI in KHT sarcoma-implanted C3H mice was performed. The effect of pentoxifylline (100 mg/kg) on reducing tumor hypoxia was also studied. Results: The radiochemical purity (RCP) of the {sup 99m}Tc-BnAO-NI preparation was greater than 96% and stable at ambient temperature for 24 h (RCP>90%). The accumulation of {sup 99m}Tc-BnAO-NI and {sup 99m}Tc-BnAO in KHT cells under hypoxia were 3.57 and 4.13-fold higher than those under normoxic environment, indicating unambiguous oxygen-dependent uptakes of these two probes. The distribution of {sup 99m}Tc-BnAO-NI in KHT sarcoma-bearing mice revealed rapid clearance from the blood circulation. The tumor uptake peaked at 2 h post-injection (0.32{+-}0.05%ID/g) with tumor-to-blood and tumor-to-muscle ratios of 10.32 and 3.96, respectively. The effect of pentoxifylline on the

  4. Evaluation of usefulness of [sup 99m]Tc-GSA liver scintigraphy on fatty liver in the rat

    Energy Technology Data Exchange (ETDEWEB)

    Kimoto, Mitsunori; Akaki, Shiro; Kohno, Yoshihiro; Gohbara, Hideo; Sakae, Katsuyoshi; Nagaya, Isao; Takeda, Yoshihiro; Hiraki, Yoshio (Okayama Univ. (Japan). School of Medicine)

    1994-10-01

    [sup 99m]Tc-GSA is a new liver-imaging radiopharmaceutical which binds to the asialoglycoprotein receptors on the hepatocytes. We evaluated liver injury induced by fatty infiltration in the rats. Studies were performed in the Wistar rats under control conditions (6 cases), and with choline deficiency diet for 2, 4, 6, 10 and 12 weeks (6 cases respectively). [sup 99m]Tc-GSA was administered via the inferior vena cava. Immediately after injection, a dynamic imaging study was performed for 30 min. t[sub 90] (the time at which the liver time-activity curve reached 90% of its peak), K[sub u] and K[sub d] (calculated by 2 compartment model) were used as parameters which reflect on asialoglycoprotein receptors on the hepatocytes. t[sub 90] prolonged, and K[sub u] and K[sub d] decreased according to the severity of fatty infiltration. These results suggest that [sup 99m]Tc-GSA is useful for evaluating liver injury induced by fatty infiltration. (author).

  5. Anti-MUC1 aptamers: radiolabelling with {sup 99m}Tc and biodistribution in MCF-7 tumour-bearing mice

    Energy Technology Data Exchange (ETDEWEB)

    Da Pieve, Chiara [Department of Chemistry and Analytical Sciences, Open University, Walton Hall, MK7 6AA Milton Keynes (United Kingdom); Perkins, Alan C. [Academic Medical Physics, School of Clinical Sciences, University of Nottingham, NG7 2UH Nottingham (United Kingdom); Missailidis, Sotiris [Department of Chemistry and Analytical Sciences, Open University, Walton Hall, MK7 6AA Milton Keynes (United Kingdom)], E-mail: s.missailidis@open.ac.uk

    2009-08-15

    Introduction: Aptamers previously selected against the protein core (AptA) or the tumour glycosylated (AptB) MUC1 glycoprotein have been conjugated to MAG2 and labelled with {sup 99m}Tc, for the potential use as radiopharmaceuticals for diagnostic imaging of breast cancer. Methods: The conjugation was achieved in high yield using standard peptide coupling reactions between an amino modification on the aptamer and the activated carboxylic group on the ligands. The retention of the affinity of the MAG2 modified AptA for the MUC1 protein core was confirmed using a fluorescent intercalator displacement binding assay. The labelled aptamers were separated from free {sup 99m}Tc using ultrafiltration and monitored by high-performance liquid chromatography at all stages, to ensure that only radiolabelled aptamers were produced. The biodistribution properties of the two aptamer-radionuclide conjugates were analysed in MCF-7 tumour bearing mice and compared. Results: Efficient and convenient labelling of the two aptamers with {sup 99m}Tc was achieved as the last step of the synthesis (post-conjugation labelling). Both the aptamer-chelator conjugates had strong {sup 99m}Tc binding properties and the resulting complexes were stable in vivo, both in terms of nuclease degradation and leaking of the metal. The radiolabelled aptamers showed a high renal clearance and a high uptake in the intestine. Conclusions: AptA and AptB have been successfully conjugated in high yield to the ligand MAG2 and labelled with {sup 99m}Tc. The radiolabelled aptamers showed different tumour uptake and clearance, but will require further development prior to diagnostic use.

  6. Investigations of 99mTc-labeled glucarate as a SPECT radiotracer for non-small cell lung cancer (NSCLC) and potential tumor uptake mechanism

    International Nuclear Information System (INIS)

    This study attempted to evaluate the feasibility of 99mTc-labeled glucarate (99mTc-GLA) imaging in non-small cell lung cancer (NSCLC) and the potential tumor uptake mechanism. Cell lysates from two NSCLC cell lines, H292 and H1975, were immunoblotted with anti-glucose transporter 5 (GLUT5) antibody for Western blotting. Thereafter, the two cell lines were used to examine cellular uptake of 99mTc-GLA with or without fructose. SPECT/CT imaging studies were performed on small animals bearing H292 and H1975 tumors. Biodistribution studies were also conducted to achieve accurate tissue uptake of this tracer in two tumor models. Hematoxylin & eosin (H&E) staining and GLUT5, Ki67 and cytokeratin-7 (CK-7) immunohistochemistry (IHC) analysis were further investigated on tumor tissues. In Western blotting, H292 cells showed higher levels of GLUT5 compared to the H1975 cells. Meanwhile, the in vitro cell assays indicated GLUT5-dependent uptake of 99mTc-GLA in H292 and H1975 cells. The fructose competition assays showed a significant decrease in 99mTc-GLA uptake by H292 and H1975 cells when fructose was added. The 99mTc-GLA accumulation was as much as two-fold higher in H292 implanted tumors than in H1975 implanted tumors. 99mTc-GLA exhibited rapid clearance pharmacokinetics and reasonable uptake in human NSCLC H292 (1.69 ± 0.37 ID%/g) and H1975 (0.89 ± 0.06 ID%/g) implanted tumors at 30 min post injection. Finally, the expression of GLUT5, Ki67 and CK-7 on tumor tissues also exhibited positive correlation with the in vitro cell test results and in vivo SPECT/CT imaging results in xenograft tumors. Both in vitro and ex vivo studies demonstrated that the uptake of 99mTc-GLA in NSCLC is highly related to GLUT5 expression. Imaging and further IHC results support that 99mTc-GLA could be a promising SPECT imaging agent for NSCLC diagnosis and prognosis evaluation

  7. Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI6]+

    Directory of Open Access Journals (Sweden)

    D.P. Faria

    2015-01-01

    Full Text Available Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [99mTc(MIBI6]+ as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol:chloroform (25:75, Whatman 3MM paper and ethanol:chloroform (25:75, and the more expensive ITLC-SG and 1-propanol:chloroform (10:90 were suitable systems for the direct determination of radiochemical purity of [99mTc(MIBI6]+ since impurities such as 99mTc-reduced-hydrolyzed (RH, 99mTcO4 - and [99mTc(cysteine2]- complex were completely separated from the radiopharmaceutical, which moved toward the front of chromatographic systems while impurities were retained at the origin. The time required for analysis was 4 to 15 min, which is appropriate for nuclear medicine routines.

  8. Genotoxic effect of radio marked lymphocytes using Tc-99m complexes; Efecto genotoxico del radiomarcado de linfocitos empleando complejos de Tc-99m

    Energy Technology Data Exchange (ETDEWEB)

    Pedraza L, M.; Ferro F, G.; Mendiola C, M.T.; Morales R, P. [Instituto nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1997-07-01

    The genotoxic effect of radio marked lymphocytes was evaluated using {sup 99m}-Tc-HMPAO and {sup 99m}-Tc- gentisic acid complexes. With the results of this work it is pretended to contribute to the knowledge of genetic and structural damages that provokes the radiation in the marked lymphocytes. The d, 1-HMPAO was synthesized in laboratory with a yielding of 30 %. The radiochemical purity of the complexes was greater than 85%. Mouse lymphocytes obtained of sanguineous volumes 2 ml were used. The radio marked efficiency of cells was 19.6 {+-} 6.4% and 25.6 {+-} 5.8% for {sup 99m}Tc-HMPAO and {sup 99m} Tc gentisic acid respectively. The genotoxic effect was evaluated using the technique of Unicellular Electrophoresis in Micro gel (Comet assay). The results showed that both {sup 99m} Tc complexes produce genotoxicity due to their capacity to penetrate cells, therefore the Auger and M internal conversion electrons place all their energy obtaining doses of Gray order. (Author)

  9. Comparison of Tc-99m pertechnetate images with dual-phase Tc 99m MIBI and SPECT images in primary hyperparathyroidism

    Directory of Open Access Journals (Sweden)

    Sait Sager

    2014-01-01

    Full Text Available Background: The purpose of this study was to evaluate the value of Tc-99m pertechnetate planar, dual-phase MIBI and MIBI-SPECT images in the determination and localization of parathyroid lesions. Materials and Methods: In this study, 38 patients who underwent operation for primary hyperparathyroidism were included. Tc-99m pertechnetate planar-pinhole imaging of the neck and then MIBI planar and SPECT images in supine position was performed. Late SPECT images were acquired 120 minutes after the injection. Early and late MIBI images were quantitatively evaluated. Results: Of the 38 patients, 30 of them had adenoma, 2 patients had hyperplasia and 6 of them were normal on planar images. Thirty-four of 38 patients were positive on SPECT images. SPECT images of the patients with positive results were matched with pathology results. Conclusion: As a result, Tc-99m pertechnetate planar-pinhole, Tc-99m MIBI planar and SPECT images are useful for localization of parathyroid lesions especially in multinodular thyroid gland. However, US or CT images are necessary for more accurate localization and Tc-99m pertechnetate images are useful for interpreting and comparing with the early and late MIBI images.

  10. Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [{sup 99m}Tc(MIBI){sub 6}]{sup +}

    Energy Technology Data Exchange (ETDEWEB)

    Faria, D.P.; Buchpiguel, C.A.; Marques, F.L.N., E-mail: danielefaria1@gmail.com [Universidade de Sao Paulo (USP), Sao Paulo, SP (Brazil). Faculdade de Medicina. Departamento de Radiologia. Servico de Medicina Nuclear

    2015-10-15

    Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [{sup 99m}Tc(MIBI){sub 6}]{sup +} as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol: chloroform (25:75), Whatman 3MM paper and ethanol:chloroform (25:75), and the more expensive ITLC-SG and 1-propanol: chloroform (10:90) were suitable systems for the direct determination of radiochemical purity of [{sup 99m}Tc(MIBI){sub 6}]{sup +} since impurities such as {sup 99m}Tc-reduced-hydrolyzed (RH), {sup 99m}TcO4{sup -} and [{sup 99m}Tc(cysteine){sub 2}]{sup -} complex were completely separated from the radiopharmaceutical, which moved toward the front of chromatographic systems while impurities were retained at the origin. The time required for analysis was 4 to 15 min, which is appropriate for nuclear medicine routines. (author)

  11. Diversification of 99Mo/99mTc separation: non–fission reactor production of 99Mo as a strategy for enhancing 99mTc availability.

    Science.gov (United States)

    Pillai, Maroor R A; Dash, Ashutosh; Knapp, Furn F Russ

    2015-01-01

    This paper discusses the benefits of obtaining (99m)Tc from non-fission reactor-produced low-specific-activity (99)Mo. This scenario is based on establishing a diversified chain of facilities for the distribution of (99m)Tc separated from reactor-produced (99)Mo by (n,γ) activation of natural or enriched Mo. Such facilities have expected lower investments than required for the proposed chain of cyclotrons for the production of (99m)Tc. Facilities can receive and process reactor-irradiated Mo targets then used for extraction of (99m)Tc over a period of 2 wk, with 3 extractions on the same day. Estimates suggest that a center receiving 1.85 TBq (50 Ci) of (99)Mo once every 4 d can provide 1.48-3.33 TBq (40-90 Ci) of (99m)Tc daily. This model can use research reactors operating in the United States to supply current (99)Mo needs by applying natural (nat)Mo targets. (99)Mo production capacity can be enhanced by using (98)Mo-enriched targets. The proposed model reduces the loss of (99)Mo by decay and avoids proliferation as well as waste management issues associated with fission-produced (99)Mo.

  12. Effect of passiflora quadrangularis extracts on the technetium-99m biodistribution in mice; Efeito de extratos do maracuja-acu na biodistribuicao do tecnecio-99m em camundongos

    Energy Technology Data Exchange (ETDEWEB)

    Bezerra, A.L.; Souza, G.M.L.; Nascimento, E.V.; Carvalho, E.B.; Catanho, M.T.J.A. [Pernambuco Univ., Recife, PE (Brazil). Dept. de Biofisica] E-mail: mariajansem@hotmail.com

    2002-07-01

    Medicinal plants have been evaluated and their effect on the biodistribution of radiopharmaceuticals are very important. This work aims to evaluate the interaction of extracts of Passiflora quadrangularis on the biodistribution of {sup 99m}Tc in mice (Mus musculus). The animals were submitted to brief treatment with aqueous (G1) and hydroalcoholic 10% (G2) extracts. Control animals received saline solution 0.9% (n=5). The reaction {sup 99m}Tc (3.7 MBq) in the presence of stannous chloride and extracts was incubated for 10 minute, then it was injected (ip way) in mice. At the end of 30 minutes, animals were sacrificed for removal of organs and 0.5 mL of blood, which were counted in gamma counter. The percentage of {sup 99m}Tc-binding was calculated in relation to the weight of each organ. The results show a reduction on right and left kidneys in the presence of aqueous extract at about 26.74%, and more significant reduction in the presence of hydroalcoholic 10% extract at about 72.81%. the trachea presents a binding capacity to {sup 99m}Tc in order of 263.5% in the presence of aqueous extract. These results demonstrate that {sup 99m}Tc presents affinity to some organs and that the extract of Passiflora quadrangularis is able to alter this labeling. Therefore, more attention has been given to diagnoses with pharmaceutical compounds labeled with radioisotopes. (author)

  13. Diversification of 99Mo/99mTc separation: non–fission reactor production of 99Mo as a strategy for enhancing 99mTc availability.

    Science.gov (United States)

    Pillai, Maroor R A; Dash, Ashutosh; Knapp, Furn F Russ

    2015-01-01

    This paper discusses the benefits of obtaining (99m)Tc from non-fission reactor-produced low-specific-activity (99)Mo. This scenario is based on establishing a diversified chain of facilities for the distribution of (99m)Tc separated from reactor-produced (99)Mo by (n,γ) activation of natural or enriched Mo. Such facilities have expected lower investments than required for the proposed chain of cyclotrons for the production of (99m)Tc. Facilities can receive and process reactor-irradiated Mo targets then used for extraction of (99m)Tc over a period of 2 wk, with 3 extractions on the same day. Estimates suggest that a center receiving 1.85 TBq (50 Ci) of (99)Mo once every 4 d can provide 1.48-3.33 TBq (40-90 Ci) of (99m)Tc daily. This model can use research reactors operating in the United States to supply current (99)Mo needs by applying natural (nat)Mo targets. (99)Mo production capacity can be enhanced by using (98)Mo-enriched targets. The proposed model reduces the loss of (99)Mo by decay and avoids proliferation as well as waste management issues associated with fission-produced (99)Mo. PMID:25537991

  14. Study on technetium-99m labeling of graphene oxide nanosheets through click chemistry-99mTc labeling of graphene oxide nanosheets

    Institute of Scientific and Technical Information of China (English)

    江大卫; 彭程; 孙艳红; 贾丽娜; 李剑波; 张岚

    2015-01-01

    Graphene oxide (GO) nanosheets possess several advantages, such as a large surface, outstanding bio-compatibility, and straightforward chemical modification capability. They also have great potential as a drug-carrier. In this article, we radiolabeled GO nanosheets with 99mTc, which satisfies the potential needs of micro-SPECT imaging probes in pre-clinical and clinical research. GO nanosheets were synthesized through the modified Hummers’ method, then GO nanosheets with azide group covalently functionalized in two steps were conjugated to DOTA (1,4,7,10-tetraazacyclododecane-N,N0,N00,N000-tetraacetic acid) and functionalized with an alkynyl group by means of click chemistry. Then through the addition and reduction of technetium-99m, the 99mTc-DOTA-GO were attained. DOTA-conjugated GOs with lateral dimensions of 500–600 nm were synthe-sized. Both atomic force microscopy (AFM) and FT-IR were performed to characterize the GO-DOTA. Labeling efficiency of GO-DOTA with 99mTc was>90%and radiochemical purities were>96%with purification. We successfully synthesized graphene oxide derivatives, DOTA-conjugated GOs, via Click Chemistry, and it was labeled with 99mTc for SPECT imaging. High radiolabeling efficiency makes GO nanosheets suitable platforms for future molecular imaging research.

  15. Biokinetic and dosimetry of {sup 99m}Tc-14F7-monoclonal antibody in patients with breast cancer

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, Joaquin; Calderon, Carlos; Pimentel, Gilmara; Oliva, Juan P., E-mail: jgg@infomed.sld.cu [Departmento de Medicina Nuclear, Instituto de Oncologia y Radiobiologia, Cidad Habana (Cuba); Casaco, Angel [Division de Ensayos Clinicos, Centro de Inmunologia Molecular, Reparto Siboney, Ciudad Habana (Cuba)

    2008-07-01

    Full text: The 14F7 murine MAb is an IgG1 that binds specifically to GM3(Neu-Gc) ganglioside strongly recognizing the antigen displayed in human breast and melanoma tumors. A preliminary report showed in vivo evidences of presence of GM3(Neu-Gc) ganglioside in human breast primary tumors using 14F7 MAb labelled with {sup 99m}Tc. In this work the pharmacokinetics and biodistribution of {sup 99m}Tc-14F7 MAb in patient with breast cancer were studied. The absorbed dose in normal organ and tumors was also calculated. The patients were selected from a Phase I/II clinical trial which included 14 female patients with cytological diagnose of breast carcinoma in stage II without previous onco-specific treatment. Three groups were conformed: Group I (0.3mg, n=5), Group II (1mg, n=5) and Group III (3mg, n=4). The activity ranged in 1.11 - 1.48GBq (30- 40mCi) was intravenously administered during 1-2min. The {sup 99m}Tc-14F7 plasma clearance was determined by blood sampling at 5, 10, 20, 30min and 1, 2, 4, 8 and 24h after injection. All urine excreted by patients was collected during 24h after injection at intervals of 0-2h, 2-8h and 8-24h. Prior to injection, a transmission scan of head, thorax, abdomen and pelvis was obtained using a {sup 57}Co flood source. Anterior and posterior whole body images were acquired at 30min, 2h, 4h, 8h and 24h after injection using a Gamma Camera (SophyCamera DS7) with pinhole collimator. Static anterior and posterior images were obtained from head, thorax, abdomen, pelvis and lateral images of each breast in pendular position at 2h, 4h, 8h and 24h. Internal absorbed dose calculation was performed according to MIRD formalism using the S values for adult non-pregnant female phantom downloaded from the RADAR website. Self-absorbed tumor dose were calculated assuming those as spheres with uniform distribution of activity. The plasma clearance of radiotracer in all groups shows a monoexponential decay behaviour with biological elimination half time

  16. Scintigraphic evaluation of chronic osteomyelitis with technetium 99 m labeled polyclonal immunoglobulin

    International Nuclear Information System (INIS)

    Active chronic osteomyelitis or complicating osteomyelitis are difficult to be diagnosed by radiological imaging modalities, such as plain radiograph and CT. They frequently cause increased bone remodeling, leading to nonspecific uptake of Tc 99 m-bone scan agents and gallium-67. New radiopharmaceuticals with greater infection avidity are being developed, including the nonspecific polyclonal immunoglobulin (IgG) labeled with technetium 99 m. Tc 99 m-IgG may be available as a ready to use kit, with no reported side effects, low patient absorbed radiation dose and low cost. Material and Methods: 23 bone segments with suspected active chronic osteomyelitis or violated bone osteomyelitis were studied by Tc 99 m IgG scintigraphy. All patients underwent standard three phase bone scintigraphy using methylene diphosphonate (Tc 99 m MDP), gallium 67 scintigraphy and plain radiographs, compared with clinical evaluation and laboratory tests values. Results: Infection was found in 8 sites. Sensitivity and specificity for Tc-99 m MDP, gallium 67 and Tc 99 m IgG scintigraphy were, respectively, 88 and 36%, 75 and 73%, 88 and 82%. Conclusion: Tc-99 m IgG may be useful in the scintigraphic evaluation of osteomyelitis. (author)

  17. Imaging endocarditis with Tc-99m-labeled antibody--an experimental study: concise communication

    Energy Technology Data Exchange (ETDEWEB)

    Wong, D.W.; Dhawan, V.K.; Tanaka, T.; Mishkin, F.S.; Reese, I.C.; Thadepalli, H.

    1982-03-01

    The sensitivity and specificity of Tc-99m-labeled antibacterial antibody (Tc-99m Ab) for detecting bacterial endocarditis were evaluated in an experimental model. Rabbit-produced antistaphylococcal antibody was extracted using Rivanol and chemically labeled with Tc-99m. This Tc-99m Ab was injected intravenously in New Zealand rabbits 24 hr after producing Staphylococcus aureus endocarditis of the aortic valve. Imaging and tissue analyses were performed on the following day. All 11 animals developed S. aureus aortic-valve vegetations and showed increased uptake of Tc-99m Ab at the aortic valve, 118 times higher than at the uninfected tricuspid valve. Although high hepatic radioactivity and anatomic uncertainties interfered with in vivo delineation of these lesions, images of the excised hearts showed all affected valves. Two rabbits inoculated with Escherichia coli did not develop endocarditis and had little uptake of Tc-99m Ab, while six rabbits with enterococcal endocarditis had no uptake of the Tc-99m Ab in their vegetations. The findings suggest potential value of Tc-99m Ab on the rapid diagnosis of endocarditis.

  18. Establishment of experimental system for 99Mo/99mTc production by neutron activation method

    International Nuclear Information System (INIS)

    The research and development (R and D) on the production of 99Mo/99mTc by neutron activation method ((n, γ) method) using JMTR has been carried out in the Neutron Irradiation and Testing Reactor Center. The specific radioactivity of 99Mo by (n, γ) method is extremely low compared with that by fission method ((n,f) method), and as a result, the radioactive concentration of the obtained 99mTc solution is also low. Focusing on the solvent extraction method with methyl ethyl ketone (MEK) as the recovery method of 99mTc from 99Mo produced by (n, γ) method to solve the problem, we have developed the 99Mo/99mTc separation/extraction/concentration devices, and we have carried out the performance tests with the devices. In this report, in order to establish an experimental system for 99Mo/99mTc production, the R and D results of the system are summarized on the improvement of the devices for high-recovery rate of 99mTc, on the dissolution of the pellets, which is the high-density molybdenum trioxide (MoO3) pellets irradiated in Kyoto University Research Reactor (KUR), on the production of 99mTc, and on the inspection of the recovered 99mTc solutions. (author)

  19. Tc-99m-HMPAO brain scintigraphy. A comparative study with I-123-IMP

    Energy Technology Data Exchange (ETDEWEB)

    Nishimoto, Hitoshi; Yamamoto, Yoichi; Yoshida, Shoji; Akagi, Naoki; Ohara, Shuichi; Kamiike, Osamu; Maeda, Tomoho

    1989-04-01

    Sixteen single photon emission computed tomography (SPECT) studies with Tc-99m hexamethyl propylene amine oxime (Tc-99m HMPAO) were performed in patients with cerebral infarction (n=10), transient ischemic attack (n=2), or brain tumor (n=3). X-ray computed tomography (CT) scans and SPECT scans with N-isopropyl-p-(I-123)iodoamphetamine (I-123 IMP) were also obtained for comparison with findings of SPECT with Tc-99m HMPAO. Crossed cerebellar diaschisis (CCD) was detected in five patients (33%) on both early and delayed SPECT scans with Tc-99m HMPAO. In 8 patients, early scans with Tc-99m HMPAO tended to depict lesions more extensively when compared with CT. Early scanning with I-123 IMP was superior to that with Tc-99m HMPAO in detecting lesions. In detecting CCD, both methods were comparable to each other. In visualizing the basal ganglion, early scans with Tc-99m HMPAO were superior to scans with I-123 IMP. Tc-99m HMPAO has the advantages of short half-life period, small amounts of exposure doses, simple labeling, and prompt usage in emergency cases, such as cerebral infarction and cerebrovascular disorder. (Namekawa, K).

  20. In Vitro Evaluation of Tc-99m Radiopharmaceuticals for Gastric Emptying Studies

    Directory of Open Access Journals (Sweden)

    Türkan Ertay

    2014-02-01

    Full Text Available Objective: Gastrointestinal motility and functional motility disorders causing either delayed or accelerated gastric emptying (GE may result in similar symptoms including nausea, vomiting, early satiety, fullness, bloating, and abdominal discomfort or pain. Hence, it is important to evaluate patients for both rapid and delayed GE in the same test. The gold standard technique to measure GE is scintigraphy by radiolabeled test meals. The aim of this study was to test alternative Tc-99m agents to label eggs as the solid meal and compare to Tc-99m sulfur colloid (SC for gastric emptying studies. Methods: In search of alternative agents for gastric emptying studies, we mixed and fried eggs with four different particulate compounds (Tc-99m labeled SC, tin colloid, nanocolloid and MAA, as well as with free pertechnetate and Tc-99m DTPA. We then measured the stability of these compounds in simulated gastric juice. Results: Our experiments demonstrated that in addition to Tc-99m sulfur colloid;Tc-99m MAA, Tc-99m nanocolloid and Tc-99m tin colloid also appear to make stable complexes with eggs in acidic environment. Conclusion: Therefore, these agents may be used for gastric emptying studies which could be more practical in routine conditions.

  1. Sensitivity of [Tc-99m]methoxyisobutylisonitrile scan in patients with metastatic differentiated thyroid cancer

    NARCIS (Netherlands)

    Ronga, G.; Ventroni, G.; Montesano, T.; Filesi, M.; Ciancamerla, M.; Di Nicola, A. D.; Travascio, L.; Viestri, A. R.; Signore, A.

    2007-01-01

    Aim. The aim of the present study was to evaluate the diagnostic sensitivity of [Tc-99m]methoxyisobutylisonitrile ([Tc-99m]MIBI) in a large series of patients with metastatic differentiated thyroid carcinoma (DTC), as compared with I-131-whole body scan (WBS) and other diagnostic imaging techniques.

  2. Preparation and Biodistribution of 99mTc-Pamidronate as Bone Imaging Agent

    Directory of Open Access Journals (Sweden)

    LUO Hong-yi

    2016-02-01

    Full Text Available Labeling of Pamidronate (PAM with 99mTc was studied by a direct labeling method in the presence of SnCl2•2H2O as reducing agent. The influences of the concentration of SnCl2•2H2O, PAM concentration and pH value, reaction time on labeling yield were investigated. The optimum labeling was determined. The results showed that the radiochemistry purity of 99mTc-PAM was more than 95%. Biodistribution studies in normal mice and rats showed very high uptake of 99mTc-PAM and long retain in bone. 99mTc-PAM was washed out from the blood very quickly. In addition, considerable uptake in the kidneys indicated this complex was excreted mainly by renal pathway. On the other hand, the radioactivity in liver, lung and heart was negligible. It could be known from SPECT images that 99mTc-PAM would be an excellent bone-imaging agent. Bone uptake of 99mTc-PAM was higher than that of 99mTc-MDP in mice. This study suggested that 99mTc-PAM was a promising bone imaging agent and further study was worthwhile.

  3. Labeling of thymidine analog with an organometallic complex of technetium-99m for diagnostic of cancer: radiochemical and biological evaluation

    International Nuclear Information System (INIS)

    Thymidine analogs have been labeled with different radioisotopes due to their potential in monitoring the uncontrollable cell proliferation. Considering that the radioisotopes technetium-99m still keep a privileged position as a marker due to its chemical and nuclear properties, this dissertation was constituted by the developed of a new technique of labeling of thymidine analog with 99mTc, by means of the organometallic complex. The aims of this research were: synthesis of the organometallic complex technetium-99m-carbonyl, thymidine labeling with this precursor, evaluation of stability, and radiochemical e biological evaluation with healthy and tumor-bearing animals. The preparation of the organometallic precursor, using the CO gas, was easily achieved, as well as the labeling of thymidine with this precursor, resulting itself a radiochemical pureness of ≥ 97% and ≥ 94%, respectively. Chromatography systems with good levels of trustworthiness were used, ensuring the qualification and quantification of the radiochemical samples. The result of in vitro testing of lipophilicity disclosed that the radiolabeled complex is hydrophilic, with a partition coefficient (log P) of -1.48. The precursor complex and the radiolabeled have good radiochemical stability up to 6 h in room temperature. The cysteine and histidine challenge indicated losses between 8 and 1 1 % for concentrations until 300 mM. The biodistribution assay in healthy mice revealed rapid blood clearance and low uptake by general organs with renal and hepatobiliary excretion. The tumor concentration was low with values of 0.28 and 0.18 %ID/g for lung and breast cancer, respectively. The results imply more studies in other tumor models or the modification of the structure of the organic molecule that act like ligand. (author)

  4. p-carboxyethyl-phenylglyoxal bis(n-methylthiosemicarbazone) (CE-DTS), a bifunctional chelating agent for Tc-99m labeled monoclonal antibody

    Energy Technology Data Exchange (ETDEWEB)

    Arano, Y.; Yokoyama, A.; Furukawa, T.; Saji, H.; Endo, K.; Torizaka, K.

    1985-05-01

    In the search for bifunctional chelating agents (BCA) with better affinity, selectivity and stability as for Tc-99m, synthesis of a novel BCA containing di-thio-semicarbazone as for Tc-99m chelating site has offered interesting characteristics for the labeling of macromolecules. In the present paper, monoclonal IgG (MoAb) against human thyroglobulin was selected as a model and conditions for coupling, labeling reactions were tested along with immunological reactivity. CE-DTS was coupled to MoAb by the azido method and effect of conjugation on the MoAb immunoreactivity was followed by RIA. When CE-DTS was coupled to MoAb at the molar ratio of 1:1, no loss of its original immunoreactivity was observed. Tc-99m labeling, using the stannous ion reducing method, indicated the reaction pH as being a determinant parameter. The reducing agent prepared in tartrate buffer (pH 3) offered high yield and stable Tc-99m-CE-DTS-MoAb, as evidence by HPLC. In vivo studies in mice indicated percent of injected dose and blood clearance alike the I-131-MoAb. This good labeled state of Tc-99m-CE-DTS-MoAb was also demonstrated by using second antibody reaction in serum of mice. The newly synthesized CE-DTS offered good basis for the Tc-99m labeling of monclonal antibodies with preserved immunological properties, as desirable for the radioimmunodetection. Work with tumor related monoclonal antibodies is under progress.

  5. Technetium-99m Sestamibi and [F18]Fluorodeoxyglucose (FDG) PET in assessing patients with multiple myeloma

    International Nuclear Information System (INIS)

    Full text: The aim of the study is to evaluate the diagnostic and prognostic value of 99mTc-Sestamibi (MIBI) in the detection on bone marrow involvement in patients suffering from Multiple Myeloma (MM), its possible role in the follow-up and comparison of 99mTc-MIBI and [F18]FDG PET imaging. We present the results, for patients at our institution, that have undergone 99mTc-MIBI scans to assess MM. We considered patients (pts) with MM (in active disease, partial and chronic remission) and with monoclonal gammopathy of undetermined significance (MGUS). A subgroup of patients with MM repeated a 99mTc-MIBI scintigraphic study at least 2 months after high-dose chemotherapy. All the scans were scored semi-quantitatively according to extension and intensity of tracer uptake. Twelve patients had concurrent 99mTc-MIBI and [F18]FDG PET. The overall sensitivity of the 99mTc-MIBI scintigraphy was 92%, specificity was 96%. In the follow up of the pts treated with chemotherapy 99mTc-sestamibi closely paralleled the activity of myeloma bone disease. A multivariate analysis showed that 99mTc-MIBI uptake pattern has an added value in relation to known prognostic variables. In another study, patients showing disease progression at restaging had higher washout of 99mTc-MIBI (10 and 60 minutes) than patients in remission; disease free survival was significantly better in patients with lower washout in absence of differences in therapeutic regimen and stage of disease at admission. [F18]FDG-PET and MIBI were concordant in seven of 12 cases (58%); only in one case FDG detected bone marrow sites in addition to those detected by 99mTc-MIBI. Our experience indicates that 99mTc-MIBI scintigraphy closely reflects myeloma disease activity in the bone marrow, and that a negative 99mTc-MIBI scan in pts with suspected MM indicates absence of disease or clinical remission. The results of these studies suggest a potential role of 99mTc-MIBI washout in predicting response to chemotherapy in

  6. Preparation and bioevaluation of 99mTc-carbonyl complex of guanine

    International Nuclear Information System (INIS)

    The aim of this study is to prepare radiolabeled guanine with 99mTc(CO)3+ core. For this purpose, guanine has been radiolabeled with 99mTc(CO)3+ core. Quality control study of radiolabeled guanine molecule with 99mTc(CO)3+ core was performed by thin layer radio chromatography (TLRC) and high performance liquid radio chromatography (HPLRC). The results showed that the radiolabeling yield was quite high (94 ± 3%). Beside that 99mTc(CO)3-Gua complex has showed good in vitro stability during the 24 h period. Radiopharmaceutical potential of this complex was evaluated in Wistar Albino Rats. It was concluded that 99mTc(CO)3-Gua could be used as a nucleotide radiopharmaceutical for in vivo applications. (author)

  7. Preparation and evaluation of (99m)Tc-DMSA lyophilized kit for renal imaging.

    Science.gov (United States)

    Jan, Syed Umer; Abbass, Hafiz Ghulam

    2013-05-01

    Dimercaptosuccinic acid (DMSA) has been evaluated and used with technetium 99m ((99m)Tc) in imaging of kidneys. DMSA lyophilized kits were prepared and radiolabelled with (99m)Tc. Paper and thin-layer chromatography have been employed using various eluent systems for the radiochemical analysis, percentage labeling and binding capacity of (99m)Tc-DMSA. Female albino rabbits were used for this study. Biological data obtained after intravenous injection of radiolabelled DMSA to female albino rabbits revealed 32.42% uptake and long retention time in the kidneys. On the basis of animal biodistribution data, it is suggested that DMSA when labeled with (99m)Tc is useful complex for renal imaging and can be successfully applied as a diagnostic tool in nuclear medicine. Clinical biodistribution and radiation dosimetry studies are planned in future.

  8. Effect of iron deficiency anemia on the biodistribution of 99mTc radiopharmaceuticals

    International Nuclear Information System (INIS)

    The distribution of colloids and labeled cells in organs is influenced by their intrinsic properties and by the state of the investigated subject. Iron deficiency remains an unsolved nutritional problem all over the world; one of its severe consequences is anemia. Because iron metabolism principally takes place in the liver, spleen, bone marrow, skeletal muscle and blood, we studied the effect of iron deficiency anemia on the biodistribution of 99mTc phytate, 99mTc gelatin colloid and 99mTc RBC (red blood cells labeled with 99mTc). Our results show that iron deficiency anemia modifies the pattern of biodistribution of the two colloids assayed. However, this behavior is different for both of them. This work contributes to studies that kinetically and statistically establish that iron deficiency anemia induces a significant inversion in the spleen-liver activity relationship when centellographic studies are performed with colloids such as 99mTc phytate

  9. A STUDY ON THE PREPARATION AND BIOLOGICAL PROPERTIES OF 99mTc-MAMA-UA AND 99mTcN-MAMA-UA%99mTc-MAMA-UA与99mTcN-MAMA-UA的制备及生物性能的比较

    Institute of Scientific and Technical Information of China (English)

    吴战宏; 王学斌; 李至敏

    2003-01-01

    首次制备了2种脂肪酸标记物99mTc-MAMA-UA和99mTcN-MAMA-UA,放化纯均大于90%.对标记物进行了小鼠的生物分布实验,结果表明二者都有一定的心肌摄取;99mTc-MAMA-UA的心肌初始摄取要大于99mTc-MAMA-HA.这说明碳链的适当增长会改变99mTc-MAMA-FA(fatty acid)的生物分布.

  10. Synthesis and preliminary biological evaluation of a technetium-99m labeled thymidine analog

    Institute of Scientific and Technical Information of China (English)

    Chun Xiong Lu; Zheng Wu Wang; Quan Fu Jiang; Jie Tang; Cheng Tan; Jian Kang Zhang

    2011-01-01

    The synthesis and labeling of 99mTc-N3-{N'-[2-sulfanyl-ethylamino)acetyl]-2-aminoethyl-sulfanyl-l-hexanamide}thymidine (99mTc-NHT) were studied. In the presence of sodium glucoheptonate (GH) and ethylene diamine tetraacetic acid (EDTA), 99mTc-NHT was obtained by using bisaminoethanethiol (N2S2) as a bifunctional coupling agent. The radiochemical purity of the 99mTc-NHT was over 95%. Biodistribution of 99mTc-NHT was performed in hepatoma HepA tumor-bearing mice. At 2 h p.i., the ratios of tumor-to-muscle, tumor-to-bone and tumor-to-blood were 4.41 ± 0.32, 2.45 ± 0.24 and 1.51 ±0.18, respectively.

  11. Renal hyperconcentration of /sup 99m/Tc-HEDP in experimental acute tubular necrosis

    International Nuclear Information System (INIS)

    The effect of transient renal ischemia on renal concentration and distribution of /sup 99m/Tc-HEDP, /sup 99m/Tc-DMSA, and /sup 99m/Tc-DTPA was compared in rabbits with acute tubular necrosis. Scintigrams were obtained after injection in normal rabbits or ones with unilateral or bilateral ischemia. /sup 99m/Tc-HEDP concentration in ischemic tissue was 8 to 18 times normal 1 to 4 hrs after injection, and the resulting images delineated the morphological changes in the ischemic kidneys more accurately than those obtained with DMSA or DTPA. Calcium concentration in the ischemic kidneys increased sixfold. /sup 99m/Tc-HEDP may be useful in evaluation of renal failure secondary to tubular injury

  12. Synthesis and biodistribution of {sup 99m}Tc-Vancomycin in a model of bacterial infection

    Energy Technology Data Exchange (ETDEWEB)

    Roohi, S.; Mushtaq, A. [Isotope Production Div., Pakistan Inst. of Nuclear Science and Technology, Islamabad (Pakistan); Malik, S.A. [Dept. of Biological Sciences, Quaid-e-Azam Univ., Islamabad (Pakistan)

    2005-07-01

    Vancomycin Hydrochloride is an antibiotic produced by the growth of certain strains of Streptomyces orientalis. As vancomycin hydrochloride is poorly absorbed after oral administration; it is given intravenously for therapy of systemic infections. Vancomycin was labeled with technetium-99m pertechnetate using SnCl{sub 2} . 2H{sub 2}O as reducing agent. The labeling efficiency depends on ligand/reductant ratio, pH, and volume of reaction mixture. Radiochemical purity and stability of {sup 99m}Tc-Vancomycin was determined by thin layer chromatography. Biodistribution studies of {sup 99m}Tc-Vancomycin were performed in a model of bacterial infection in Sprague-Dawley rats. A significantly higher accumulation of {sup 99m}Tc-Vancomycin was seen at sites of S. aureus infected animals. Whereas uptake of {sup 99m}Tc-Vancomycin in turpentine inflamed rats were quite low. (orig.)

  13. 99mTc-YIGSR as a Receptor Tracer in Imaging the Ehrlich Ascites Tumor-bearing Mice as Compared with 99mTc-MIBI

    Institute of Scientific and Technical Information of China (English)

    HU Jia; QIN Guangming; ZHANG Yongxue; AN Rui; LAN Xiaoli

    2007-01-01

    The validity of 99mTc-YIGSR, a novel receptor radio-tracer, in imaging the Ehrlich ascites tumor was evaluated. YIGSR, a pentapeptide of laminin, was labeled with 99mTc by using a bifunctional chelator S-Acetly-NH3-MAG3. The MIBI was labeled with 99mTc by following the kit instruction. The mice of tumor group were intravenously injected 1-2 mCi of 99mTc-YIGSR or 99mTc-MIBI via caudal vein, immobilized and imaged under a Gamma camera. The same procedure was performed in mice of blockade group, in which the unlabeled YIGSR was previously injected to block the receptor-recognition sites, and inflammation group serving as control. The reverse-phase Sep-Pak C18 chromatogram was found to have an essentially complete conjugation between YIGSR and S-Acetly-NH3-MAG3. The conjugated YIGSR could be radio-labeled successfully with 99mTc at room temperature and neutral pH, with a radio-labeling yield of 62%. Without the chelator S-Acetly-NH3-MAG3, the YIGSR was labeled with 99mTc at an efficiency of 4%. The imagological study revealed obvious tumor accumulation of 99mTc-YIGSR 15 min after the injection, and the uptake peaked after 3 h with a tumor-to-muscle ratio (T/M) of 11.36. The radio-tracer was slowly cleared up and resulted in a T/M of 3.01 at the 8th h after the injection. As for blocked group, the tumor uptake of radiotracer was significantly lower, with the highest T/M being 4.61 after 3 h and 0.89 after 8 h. The T/M was 3.72 at the 3rd h and 1.29 at the 8th h after the 99mTc-YIGSR injection in the inflammatory group. The T/M was significantly higher in tumor group than in inflammatory group or control group (P<0.001). In the 99mTc-MIBI group, the T/M was 1.40 at the 3rd h and 0.55 at the 8th h after the injection, which showed a significant difference as compared with 99mTc-YIGSR (P<0.001).It is concluded that YIGSR can be successfully radiolabelled by using S-Acetly-NH3-MAG3.99mTc-YIGSR has many advantages in tumor imaging, such as quick and clear visualization

  14. Capillary permeability of 99mTc-DTPA in canine myocardium determined by intracoronary bolus injection and residue detection

    DEFF Research Database (Denmark)

    Svendsen, Jesper Hastrup; Efsen, F; Haunsø, S

    1989-01-01

    Capillary permeability of 99mtechnetium-diethylenetriaminepenta-acetic acid (99mTc-DTPA; MW 485.0) and 51chromium-ethylenediaminetetra-acetate (51Cr-EDTA; MW 340.2) was studied in an in vivo canine heart preparation by the single injection, residue detection (SIRD) method. In experiments on open...... chest dogs (group A) these indicators were administered separately as bolus injections into a cannulated diagonal branch of the left anterior descending coronary artery (LAD) and the curve of the response function was recorded by external activity registration. In further experiments on closed and open...

  15. Value of Tc99m-DTPA alveolar permeability in lung involvement detection of patients with HIV infection

    International Nuclear Information System (INIS)

    We studied 35 HIV patients in order to know the value of Tc99mDTPA in the assessment of pulmonary lung involvement, especially pneumocystis carinii (PC) infection. Lung DTPA clearance measures increased alveolar permeability. Twenty patients with respiratory symptoms were included, 4 with systemic symptoms and also 11 asymptomatics, with similar immune condition (CD4 lymphocytes <400) as a control group. Smoking habit was suspended prior the test. Clinical follow up, chest film, induced sputum and/or fibrobronchoscopy were obtained. There was histological confirmation of PC presence or absence in 16 symptomatics and 3 asymptomatics. DTPA sensitivity for PC detection was 78%, specificity 40% and accuracy 58%; the values were 85%, 60% and 79%, respectively, for inflammatory lung processes. There were 4/6 cases false positive for PC detection with respiratory features explaining DTPA abnormalities. Concluding, Tc99m-DTPA is sensitive but not specific for detecting PC pneumonia but its value is higher for pulmonary inflammatory processes (Au)

  16. Preparation of 99mTc-PQQE and preliminary biological evaluation for the NMDA receptor

    International Nuclear Information System (INIS)

    The 4, 5-dioxo-4, 5-dihydro-1H-pyrrolo(2, 3-f)quinoline-2, 7, 9-tricarboxylic acid 2-ethyl ester 7, 9-dimethyl ester (PQQE) was synthesized on the basis of Pyrroloquinoline quinine (PQQ). 99mTc-PQQE was prepared using stannous fluoride (SnF2) as reducing agent. Biological characteristics of 99mTc-PQQE include lipophilic and the charge properties were compared to 99mTc-PQQ. The biodistributions of 99mTc-PQQE in mice and brain regional distribution were performed. In vivo distribution of 99mTc-PQQE in mice indicates that the concentration ratio of drug and blood increases steadily over time. The major radioactivity may be metabolized by the hepatic and renal system. The elimination-phase half-time (t1/2 β) results indicate that the residence time of 99mTc-PQQE (203.92) in the body is twice as long as 99mTc-PQQ (100.45)., The uptake of 99nTc-PQQE in brain was improved due to the ameliorating of charge and lipophilicity. The highest total regional brain uptake of 99mTc-PQQE was in the frontal lobe and hippocampus, where the NMDA receptor is very abundant. 99mTc-PQQE had a good target to nontarget ratio (hippocampus/cerebellum) which preserved a higher value (peak 4.0 at 120 min) from 60 min to 180 min after injection. In vitro autoradiographic results are in close agreement with the regional brain map. The enrichment can be blocked by N-methyl-D-aspartate receptor (NMDAR) redox modulatory site antagonists-ebselen (EB). This work suggests that 99mTc-PQQE has some specific targeting to the NMDA receptor. (authors)

  17. Bone turnover markers are correlated with total skeletal uptake of 99mTc-methylene diphosphonate (99mTc-MDP)

    International Nuclear Information System (INIS)

    Skeletal uptake of 99mTc labelled methylene diphosphonate (99mTc-MDP) is used for producing images of pathological bone uptake due to its incorporation to the sites of active bone turnover. This study was done to validate bone turnover markers using total skeletal uptake (TSU) of 99mTc-MDP. 22 postmenopausal women (52–80 years) volunteered to participate. Scintigraphy was performed by injecting 520 MBq of 99mTc-MDP and taking whole body images after 3 minutes, and 5 hours. TSU was calculated from these two images by taking into account the urinary loss and soft tissue uptake. Bone turnover markers used were bone specific alkaline phosphatase (S-Bone ALP), three different assays for serum osteocalcin (OC), tartrate resistant acid phosphatase 5b (S-TRACP5b), serum C-terminal cross-linked telopeptides of type I collagen (S-CTX-I) and three assays for urinary osteocalcin (U-OC). The median TSU of 99mTc-MDP was 23% of the administered activity. All bone turnover markers were significantly correlated with TSU with r-values from 0.52 (p = 0.013) to 0.90 (p < 0.001). The two resorption markers had numerically higher correlations (S-TRACP5b r = 0.90, S-CTX-I r = 0.80) than the formation markers (S-Total OC r = 0.72, S-Bone ALP r = 0.66), but the difference was not statistically significant. TSU did not correlate with age, weight, body mass index or bone mineral density. In conclusion, bone turnover markers are strongly correlated with total skeletal uptake of 99mTc-MDP. There were no significant differences in correlations for bone formation and resorption markers. This should be due to the coupling between formation and resorption

  18. Splenic sequestration of Tc-99m labeled heat treated red blood cells. [Feasibility of use for spleen imaging

    Energy Technology Data Exchange (ETDEWEB)

    Atkins, H L; Goldman, A G; Fairchild, R G; Oster, Z H; Som, P; Richards, P; Meinken, G E; Srivastava, S C

    1979-01-01

    The rate of blood clearance and spleen uptake as well as the total spleen uptake of heat damaged red blood cells labeled with Tc-99m was determined in eight patients, six of whom had chronic lymphatic leukemia, one had polycythemia vera and one had eosinophilia of unknown origin. Spleen uptake at 2 hrs was 72.0 +- 18.5%. Approximately 82.6% of the initial radioactivity was cleared from the blood by 2 hrs with a rapid T 1/2 component of 6.3 +- 4.7 min. The T 1/2 of splenic uptake was 8.3 +- 4.6 min with a plateauing of splenic radioactivity by 30 min. The preliminary results indicate that the method of preparation is reliable but the usefulness of the method for evaluating spleen function remains to be determined.

  19. The preparation of {sup 99m}Tc from {sup 99}Mo-{sup 99m}Tc using Poly-Zirconium Compound (PZC)

    Energy Technology Data Exchange (ETDEWEB)

    Laohawilai, S. [Isotope Production Div., Office of Atomic Energy for Peace (Thailand)

    1998-10-01

    The process of for providing the {sup 99m}Tc-gel generator followed the protocol that received from Department of Radioisotopes, Tokai Research Establishment, JAERI. Sodium molybdate (molybdenum-99), pH 7 with concentration 20 mg/ml and activity nearly 1 mCi/ml was added into the adsorbent (PZC). The total activity of {sup 99}Mo was about 10 mCi and the heating time was varied from 3-18 hours at 70degC. The properties of the gel were studied for elution profile and elution efficiency. The molybdenum breakthrough in sodium pertechnetate (technetium-99m) was also performed. (author)

  20. [Diagnostic possibilities of static gamma-scintigraphy using 99mTc pyrophosphate and Tc 99m phosphate in children with glomerulo nephritis].

    Science.gov (United States)

    Mil'ko, V I; Bagdasarova, I V; Moskalenko, N I; Ivanov, D D

    1989-01-01

    Children with nephrotic glomerulonephritis were examined for incorporation of 99mTc-pyrophosphate and 99mTc-phosphone. The relationship was discovered between the accumulation of the radiopharmaceutical drug and the sensitivity to glucocorticoids used in the pathogenetic treatment. Accumulation of the drug in the course of the treatment documents the presence of complete, partial remission or the disease activity. It is recommended that static gammascintigraphy should be carried out for early assessment of the efficacy of the treatment and its correction.

  1. Lung function declines in patients with pulmonary sarcoidosis and increased respiratory epithelial permeability to 99mTc-DTPA

    International Nuclear Information System (INIS)

    Respiratory epithelial clearance of 99mTc-DTPA (RC-Tc-DTPA) and pulmonary function tests (PFT) were determined at intervals of 6 or 12 months in 37 untreated, nonsmoking patients with sarcoidosis over a period of 6 to 36 months. PFT included the measurements of total lung capacity (TLC), vital capacity (VC), FEV1, and diffusing capacity for carbon monoxide. No difference was found between the respiratory clearance of 113mIn-DTPA (2.25 +/- 1.00%/min) and RC-Tc-DTPA (2.29 +/- 1.11%/min) in eight patients with pulmonary sarcoidosis. Pulmonary function decreased 15% or more in at least 2 function tests during 11 follow-up periods, but it remained stable during 47 follow-up periods. In patients whose lung function deteriorated, RC-Tc-DTPA increased to 3.51 +/- 1.55%/min; in contrast, in patients whose lung function remained stable, regardless of the initial values, RC-Tc-DTPA was normal (1.00 +/- 0.50%/min; p less than 0.001). In eight patients who were treated with corticosteroids, RC-Tc-DTPA decreased from 3.48 +/- 1.31%/min to 1.56 +/- 0.64%/min (p less than 0.001), and PFT improved. We conclude that in nonsmokers with pulmonary sarcoidosis, increased RC-Tc-DTPA is not related to dissociation of 99mTc from DTPA, RC-Tc-DTPA is increased when pulmonary function decreases, and, when increased, RC-Tc-DTPA decreases with corticosteroid therapy

  2. Preparation, characterization, and biodistribution study of technetium-99m labelled crotalus venom

    International Nuclear Information System (INIS)

    Technetium-99m (99mTc) is a radionuclide widely used for nuclear medical examination. Called the workhorse of nuclear medicine, 99mTc is the favoured choice because it has the appropriate physical and chemical characteristics for imaging. The gamma radiation emitted by 99mTc has the appropriate energy (140 keV) to provide high efficiency detection with the advantage of reduced radiation burden for the patient and environmental (half-life of 6 h). Crotalus venom (CV) has been tested in a very few human cases and it has been shown to reduce tumours. Our group has shown that CV has antitumoural effects against some brain tumours in vitro. Pharmacokinetics and tissue distribution studies are very important for clinical use. The aim of the present study was to obtain an analogue of CV labelled with 99mTc which preserves its biological activity for use in biodistribution and binding studies. A direct method for 99mTc-labelling venom has been evaluated according to Pauwels, et al. The method employs stannous chloride and sodium borohydride as reducing agents. By altering the reaction conditions high yield of labelled venom was achieved. Labeling yield was estimated using chromatographic systems. Biological activity was assessed by haemolytic activity study. Comparing haemolytic activities of labelled and unlabeled venom we observed that neither 99mTc-CV nor CV caused direct lysis on washed erythrocytes. However, both of them caused indirect hemolysis provided that the incubation medium contained an exogenous source of lecithin. So, the biological activity of CV was preserved after labeling. Biodistribution of 99mTc-CV was evaluated in mice. Male swiss mice were injected i.p. with 99mTc-CV, vital organs were isolated and their respective radioactivities were measured. High radioactivity was found in the kidneys suggesting renal excretion. On the other hand, this radioactivity was displaced in animals pre-treated with excess of non labelled CV (not shown). These data show

  3. Clinical estimation of acute myocardial infarct size with /sup 99m/technetium pyrophosphate scintigraphy

    International Nuclear Information System (INIS)

    We evaluated scintigraphic techniques in estimating infarct size. In 26 patients with acute transmural myocardial infarction, /sup 99m/Technetium pyrophosphate (TcPYP) infarct scintigraphy, gated cardiac blood pool scintigraphy and 201-Thallium (201-Tl) perfusion scintigraphy were performed. Invasive hemodynamic measurements were obtained and serial venous blood specimens taken for measurement of total and MB creatine phosphokinase (CPK). Infarct size was estimated from the area of abnormal TcPYP uptake, the extent of reduced 101-Tl uptake, the percentage of abnormally contracting segments, and serial enzyme measurements. Left ventricular ejection fraction (LVEF) and stroke work index (LVSWI) were calculated. TcPYP infarct area was associated with the extent of reduced 201-Tl uptake (r = 0.66), the percentage of abnormally contracting segments (r = 0.64), and with both LVSWI (r = 0.73) and LVEF (r = -0.58). TcPYP infarct area did not correlate with cumulative total or MB-CPK release or the integrated total CPK-time curve, nor did the enzyme estimates of infarct size correlate with LVSWI or LVEF. Variable perfusion of infarcts of different sizes may explain the lack of correlation between TcPYP infarct area and enzyme estimates of infarct size. A combination of anatomic and functional indices derived from scintigraphic and hemodynamic measurements may provide the best assessment of infarct size

  4. Study on placental blood flow in late pregnancy by intravenous sup(99m)Tc method

    International Nuclear Information System (INIS)

    A method for the continuous recording of uteroplacental blood flow (PBF) in late pregnancies by using sup(99m)Tc-albumin has been described. 1) The PBF curve of toxemia of pregnancy has been plotted to indicate small artery spasm in proving ischemic necrosis of placenta. 2) In the PBF of placental insufficiency evidenced by the values for urinary E3, an unfavorable build-up and a delayed build-up time were observed. The pathologic diagnosis showed condensation, fusion and necrosis of villi. 3) In the PBF in which a intrauterine fetal death (IUFD) was caused by placental factors, a sudden change in the PBF was observed showing the presence of an ischemia. 4) In the PBF of pregnancy with diabetes, a large wave pattern change was observed indicating a decrease in the PBF. The pathologic diagnosis showed the fusion, hyalinization and necrosis of villi. 5) The PBF wave patterns were classified into four kinds: (1) normal pattern, (2) angio-spasm pattern, (3) delayed build-up pattern, (4) circulation pattern. It has become clear that these abnormal wave patterns are frequently observed in toxemia of pregnancy, placental insufficiency and pregnancy with diabetes. (author)

  5. Preparing an estrogen - derivative compound labeled with Tc-99m and determination of radiopharmaceutical potential

    International Nuclear Information System (INIS)

    In recent years, estrogen derivative compounds have been used with several purpose which may lead to rapid advances in both diagnosis and therapy of some human cancers. In this study, an estrogen derivative compound (ESTCPTA) that 3,17-β-estradiolyl propinol attached with 1-(4-methylbenzoic acid)-4,8,11- tetraazacyclotetradecane (CPTA) was synthesized at five steps. Synthesized product was purified by recrystallization in ethyl alcohol. Characterizations of purified products were made by NMR and IR spectroscopy. Synthesized compound was labeled with Tc-99m. TLC (Radio Thin Layer Chromatography) and radio-paper electrophoresis were used to determine radiochemical yields. Specific activity was approximately 23.7 GBq/mmol and labeling yield was over 95%. Biodistribution studies were performed on female Albino Wistar rats. Rats were sacrificed by ether narcotization at certain time intervals and the organs were removed. Their activities were counted by a gamma counter. The activity per gram tissue was calculated and time activity curves were generated

  6. Internal radiation absorbed dose estimation in human brain due to technetium-99m and iodine-131

    International Nuclear Information System (INIS)

    Internal dosimetry is a branch of medical physics that deals with the measurement of the internally absorbed dose by an organ after applying isotopes. In this study, internal radiation absorbed dose has been calculated for 99mTc and 131I, which are frequently used for functioning tests and therapeutic treatments of thyroid, respectively in these cases, some amount of isotopes are accumulated in other tissues like brain, which are very soft and cannot be regenerated if they are damaged. Using ionizing radiation inside the body and to ensure the safety of brain, the internal radiation absorbed dose has been calculated applying direct counting measurement. Accumulation of isotopes to target organ has been measured and this target organ is considered as primary target organ; also this organ is considered as source with respect to other organs. These organ counts have, been measured by computer-based scintillation system. The amount of exposure in brain has been measured with the help of the data obtained from the special set-up equipment, including NaI detector, radiation survey meter and water phantoms of various sizes. Absorbed dose in brain for each isotope has been calculated by applying time-activity curve analysis. Finally, these results have been compared with the data in ICRP l Reports 53 and 71. (author)

  7. Gallbladder ejection fraction using {sup 99m}Tc-DISIDA scan in diabetic autonomic neuropathy

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Seong Jang; Kim, In Ju; Kim, Yong Ki; An, Jun Hyup [Pusan National Univ. Hospital, Pusan (Korea, Republic of); Yoo, Seok Dong [Dongkuk Univ. College of Medicine, Seoul (Korea, Republic of)

    2000-02-01

    We performed this study to evaluate the changes of gallbladder ejection fraction (GBEF) in diabetic patients with or without autonomic neuropathy. This study included 37 diabetic patients (25 women, 12 men, mean age 51 years) and 24 normal controls (10 women, 14 men, mean age 38 years). After intravenous injection of 185 MBq of {sup 99m}T{sub c}-DISIDA, serial anterior abdominal images were acquired before and after fatty meal. Regions of interest were applied on gallbladder and right hepatic lobe on 60 and 90 minute images to calculate GBEF. GBEF was significantly reduced in diabetes with autonomic neuropathy (43{+-}12.3%) and without autonomic neuropathy (57.5{+-}13.2%) compared with normal controls (68{+-}11.6%, p<0.05). And also, GBEF was significantly reduced in diabetes with autonomic neuropathy compared with diabetes without autonomic neuropathy (p<0.05). Fasting blood glucose level, age, sex, hemoglobin A1c, body mass index, serum lipid level were not different in these two diabetic patient groups (p>0.05). When 50.2% of GBEF was used as the criteria for diabetic autonomic neuropathy, the sensitivity and specificity were 80%, 76.5%, respectively. The area under receiver operating characteristic curve was 0.846. GBEF of diabetic patients with autonomic neuropathy was significantly reduced than that of diabetic patients without autonomic neuropathy.

  8. Synthesis and biological evaluation of novel 99mTcN-labeled bisnitroimidazole complexes containing monoamine-monoamide dithiol as potential tumor hypoxia markers

    International Nuclear Information System (INIS)

    Tumor hypoxia can decrease the efficacy of clinical therapy due to resistance toward radiation damage and chemotherapy, thus detection of tumor hypoxia by radiolabeled hypoxia markers is important for the control of tumor. Radiopharmaceuticals with two bioreductive groups, such as propylene amine oxime-bisnitroimidazole or monoamine-monoamide dithiol (MAMA) -bisnitroimidazole, have potential to improve hypoxia selectivity. In order to obtain radiopharmaceuticals with better features, we synthesized two novel [99mTcN]2+ complexes with bisnitroimidazole moieties and MAMA ligand for targeting tumor hypoxia. Their physicochemical characters and biodistribution were also investigated. Both the [99mTcN]2+ complexes show good stability and hydrophilicity. They show faster clearance from blood and soft tissues, better tumor retention and favorable tumor-to-tissue ratios compared with a control complex without nitroimidazole group. In addition, both of them show more favorable biodistribution patterns than the corresponding [99mTcO]3+ complexes. These results indicate that the 99mTcN-labeled MAMA-bisnitroimidazole complexes would have potential to image tumor hypoxia in vivo. (author)

  9. Novel [99mTcN]2+ Labeled EGFR Inhibitors as Potential Radiotracers for Single Photon Emission Computed Tomography (SPECT Tumor Imaging

    Directory of Open Access Journals (Sweden)

    Mingxia Zhao

    2014-04-01

    Full Text Available The epidermal growth factor receptor (EGFR is overexpressed in many cancers, including breast, ovarian, endometrial and non-small cell lung cancer. An EGFR-specific imaging agent could facilitate clinical evaluation of primary tumors or metastases. To achieve this goal, 4-(2-aminoethylamino-6,7-dimethoxyquinazoline (ADMQ was synthesized based on a 4-aminoquinazoline core and then conjugated with N-mercapto- acetylglycine (MAG and N-mercaptoacetyltriglycine (MAG3, respectively, to give compounds 1 and 2. The final complexes [99mTcN]-1 and [99mTcN]-2 were successfully obtained with radiochemical purities of >99% and >98% as measured by radio-HPLC. No decomposition of the two complexes at room temperature was observed over a period of 2 h. Their partition coefficients indicated they were hydrophilic and the electrophoresis results showed they were negatively charged. Biodistribution in tumor-bearing mice demonstrated that the two new complexes showed tumor accumulation, high tumor-tomuscle (T/M ratios and fast clearance from blood and muscle. Between the two compounds, the 99mTcN-MAG3-ADMQ ([99mTcN]-2 showed the better characteristics, with the tumor/muscle and tumor/blood ratios reached 2.11 and 1.90 at 60 min post-injection, 4.20 and 1.10 at 120 min post-injection, suggesting it could be a promising radiotracer for SPECT tumor imaging.

  10. Preparation, molecular modeling and biodistribution of 99mTc-phytochlorin complex

    International Nuclear Information System (INIS)

    Phytochlorin [21H, 23H-Porphine-7-propanoicacid, 3-carboxy-5-(carboxymethyl)13-ethenyl-18-ethyl-7,8-dihydro-2,8,12,17- tetramethyl-,(7S,8S)] was labeled with 99mTc and the factors affecting the labeling yield of 99mTc-phytochlorin complex were studied in details. At pH 10, 99mTc-phytochlorin complex was obtained with a high radiochemical yield of 98.4 ± 0.6 % by adding 99mTc to 100 mg phytochlorin in the presence of 75 μg SnCl2·2H2O after 30 min reaction time. The molecular modeling study showed that the structure of 99mTc-phytochlorin complex presents nearly linear HO-Tc-OH unit with an angle of 179.27 deg and a coplanar Tc(N1N2N3N4) unit. Biodistribution of 99mTc-phytochlorin complex in tumor bearing mice showed high T/NT ratio (T/NT = 3.65 at 90 min post injection). This preclinical study showed that 99mTc-phytochlorin complex is a potential selective radiotracer for solid tumor imaging and afford it as a new radiopharmaceutical suitable to proceed through the clinical trials for tumor imaging. (author)

  11. Strategies toward the commercialization PZC-based Tc-99m generator in the Philippines

    International Nuclear Information System (INIS)

    Technetium-99m (99mTc) is the most widely used radioisotope in nuclear medicine. It accounts for more than 80% of the total demand for radioisotopes. Alone or conjugated with other ligands, it is very useful in the imaging and scanning of various organs such as the brain, lungs, kidneys, liver, thyroid and bone and in the diagnosis of metabolic disorders. It is imported to the country as 99Mo-99mTc generators. These commercial generators use fission molybdenum adsorbed onto alumina column. A Draft Business Plan for the Production of PZC 99Mo-99mTc Generator in the Philippines was presented during the FNCA 2005 Workshop on the Utilization of Research Reactors, August 08-12, 2005. The Philippine Nuclear Research Institute (PNRI) proposed in the draft business plan, the commercial production of PZC generator through the establishment of a facility for the in-house production of 99Mo-99mTc generators. The radioisotope laboratory of the Irradiation Services Unit will be renovated to house the PZC based 99Mo-99mTc generator experiment/production facility. Since the research reactor is on extended shutdown, the supply of 99Mo will be sourced out from neighboring countries. Information dissemination and promotions will be made in order to bring this PZC based 99Mo-99mTc generator to the nuclear medicine users. (author)

  12. Mapping lymph nodes in cancer management – role of 99mTc-tilmanocept injection

    Directory of Open Access Journals (Sweden)

    Tausch C

    2014-06-01

    Full Text Available Christoph Tausch, Astrid Baege, Christoph RagethBrust-Zentrum, Zürich, SwitzerlandAbstract: Two decades ago, lymphatic mapping of sentinel lymph nodes (SLN was introduced into surgical cancer management and was termed sentinel node navigated surgery. Although this technique is now routinely performed in the management of breast cancer and malignant melanoma, it is still under investigation for use in other cancers. The radioisotope technetium (99mTc and vital blue dyes are among the most widely used enhancers for SLN mapping, although near-infrared fluorescence imaging of indocyanine green is also becoming more commonly used. 99mTc-tilmanocept is a new synthetic radioisotope with a relatively small molecular size that was specifically developed for lymphatic mapping. Because of its small size, 99mTc-tilmanocept quickly migrates from its site of injection and rapidly accumulates in the SLN. The mannose moieties of 99mTc-tilmanosept facilitate its binding to mannose receptors (CD206 expressed in reticuloendothelial cells of the SLN. This binding prevents transit to second-echelon lymph nodes. In Phase III trials of breast cancer and malignant melanoma, and Phase II trials of other malignancies, 99mTc-tilmanocept had superior identification rates and sensitivity compared with blue dye. Trials comparing 99mTc-tilmanocept with other 99mTc-based agents are required before it can be routinely used in clinical settings.Keywords: lymphatic mapping, sentinel lymph node, new tracer

  13. Radioactive aerosols study: computer program proposal for processing alveoli-capillary clearance studies

    International Nuclear Information System (INIS)

    Studies on alveolar-capillary clearance (ACC) of technetium-99 m diethylene triamine penta-acetic acid (99 mTc-DTPA) allows early presumptive diagnosis of opportunistic lung infections with higher sensitivity than conventional methods. A computer program for processing such studies is developed. Acquired data are then processed by the program which corrects for radioactive decay, allows regions of interest drawing and builds time-activity curves up. The program still apply the least mean square method and gives the user the ACC rate (%/min), the best fitting equations and the time-activity graph. Thirteen non-smoker normal subjects (3 female, 10 male), 22-62 years-old (mean 34 years-old) are studied, using the developed program. (author). 123 refs, 20 figs

  14. A new technique of 99mTc-ciprofloxacin kit preparation

    International Nuclear Information System (INIS)

    Aim: Development of a simple and reproducible method for preparing a ciprofloxacin kit using a redox polymer, which would meet the requirements for an easy and reliable technique of labelling with 99mTc and diagnostic efficiency in scintigraphic imaging of infections. Material and methods: To prepare the kit, an insoluble redox polymer containing an end α(β)-alanine-N,N'-diacetate group anchored to the dextron matrix was used. The redox polymer synthesised by the authors was incubated at room temperature (10 h) with a solution of ciprofloxacin (1%) in a suspension (5%). The mixture was then filtered and dispensed into sterile vials (0.2 ml each). The kit was labelled with 99mTc for 10 min at room temperature. The radiochemical purity of the ciprofloxacin-99mTc complex was determined by ITLC and paper chromatography in relation to the following factors: pH, total content of ciprofloxacin, volume of sodium 99mTc-pertechnetate. Ciprofloxacin biodistribution was evaluated in Wistar rats with Staphylococcus aureus infection in the left inguinal region 24 h after abscess induction. Accumulation of 99mTc activity was determined both using external gamma camera imaging and counting dissected tissues 1 h after administration. Results: Radiochemical purity is >95% for kit-labelling (pH 3.3-3.7). With pH 3.45, labelled ciprofloxacin shows the highest stability and radiochemical purity. The 99mTc-ciprofloxacin complex is stable for at least 8 h. In experimentally induced inflammation, the amount of accumulated 99mTc-ciprofloxacin activity is five times higher than in controls. Conclusion: The developed method of 99mTc-ciprofloxacin kit preparation employs a redox polymer in a new procedure, which enables the preparation of a stable kit with a high 99mTc-labelling efficiency. The labelled kit is suitable for scintigraphic imaging of infection. (orig.)

  15. Novel 99mTc labeled σ receptor ligand as a potential tumor imaging agent

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A novel 99mTc labeled complex, [N-[2-((2-oxo-2-(4-(3-phenylpropyl)piperazin-1-yl)ethyl) (2-mercaptoethyl)amino)acetyl]-2-aminoethanethiolato]Technetium(V) oxide (PPPE-MAMA′-99mTcO) ([99mTc]-2) has been designed and prepared based on the integrated approach. The corresponding rhenium complex (PPPE-MAMA′-ReO)(Re-2) has been prepared and characterized. In vitro competition binding assays show moderate affinity of Re-2 towards σ1 and σ2 receptors with Ki values of 8.67 ± 0.07 and 5.71 ± 1.88 μmol, respectively. Planar images obtained at 0.5 h, 4 h, 20 h after I.v. Injection indicate the accumulation of [99mTc]-2 in MCF-7 human breast tumor bearing mice at 20 h. Furthermore, the accumulation of [99mTc]-2 has been inhibited at 20 h after co-injection of [99mTc]-2 plus haloperidol (1 mg/kg). Biodistribution studies of [99mTc]-2 display an in vivo tumor uptake of 0.14% ± 0.01% ID/g at 24 h post I.v. Injection with a tumor/muscle ratio of 6.02 ± 0.87. The above results suggest that [99mTc]-2, derived from a previously published lead compound, retains certain tumor uptake and affinity for σ receptors. [99mTc]-2 may be used as a basis for further structural modifications to develop tumor imaging agents with high affinity for σ receptors.

  16. {sup 99m}Tc-glycopeptide: Synthesis, biodistribution and imaging in breast tumor-bearing rodents

    Energy Technology Data Exchange (ETDEWEB)

    Wei, I-C. [Center for Innovation Development, Taiwan Hopax Chems Mfg. Co., Ltd., No. 28, Hua Dong Road, Daliao, Kaohsiung 83162, Taiwan (China); Tsao Ning [Division of Diagnostic Imaging, University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Box 59, Houston, TX 77030 (United States); Huang Yahui; Ho Yensheng; Wu Chungchin [Center for Innovation Development, Taiwan Hopax Chems Mfg. Co., Ltd., No. 28, Hua Dong Road, Daliao, Kaohsiung 83162, Taiwan (China); Yu Dongfang [Division of Diagnostic Imaging, University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Box 59, Houston, TX 77030 (United States); Yang, David J. [Division of Diagnostic Imaging, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Box 59, Houston, TX 77030 (United States)], E-mail: dyang@di.mdacc.tmc.edu

    2008-03-15

    This study was aimed to develop a glycopeptide (GP) to be used as a carrier for anti-cancer drug delivery. GP was synthesized by conjugating glutamate peptide and chitosan using carbodiimide as a coupling agent. Elemental analysis and capillary electrophoresis confirmed the purity was >95%. GP was labeled with sodium pertechnetate (Na{sup 99m}TcO{sub 4}) for in vitro and in vivo studies. Rhenium-GP was synthesized to support the binding site of {sup 99m}Tc at the glutamate positions 3-5. In vitro cellular uptake of {sup 99m}Tc-GP was performed in breast cancer cells. Cytosol had 60% whereas nucleus had 40% uptake of {sup 99m}Tc-GP. When cancer cells were incubated with glutamate or aspartate, followed by {sup 99m}Tc-GP, there was decreased uptake in cells treated with glutamate but not aspartate. The findings indicated that cellular uptake of {sup 99m}Tc-GP was via glutamate transporters. In addition, {sup 99m}Tc-GP was able to measure uptake differences after cells treated with paclitaxel. Biodistribution and planar imaging were conducted in breast tumor-bearing rats. Biodistribution of {sup 99m}Tc-GP showed increased tumor-to-tissue ratios as a function of time. Planar images confirmed that {sup 99m}Tc-GP could assess tumor uptake changes after paclitaxel treatment. In vitro and in vivo studies indicated that GP could target tumor cells, thus, GP may be a useful carrier for anti-cancer drug delivery.

  17. Synthesis and evaluation of a technetium-99m labeled cytotoxic bombesin peptide conjugate for targeting bombesin receptor-expressing tumors

    Energy Technology Data Exchange (ETDEWEB)

    Okarvi, Subhani M. [Cyclotron and Radiopharmaceuticals Department, King Faisal Specialist Hospital and Research Centre, MBC-03, PO Box 3354, Riyadh 11211 (Saudi Arabia)], E-mail: sokarvi@kfshrc.edu.sa; Al Jammaz, Ibrahim [Cyclotron and Radiopharmaceuticals Department, King Faisal Specialist Hospital and Research Centre, MBC-03, PO Box 3354, Riyadh 11211 (Saudi Arabia)

    2010-04-15

    Conjugation of the cytotoxic drugs to receptor-binding peptides is an attractive approach for the targeted delivery of cytotoxic peptide conjugates to tumor cells. In an attempt to develop an efficient peptide-based radiopharmaceutical for targeting bombesin (BN) receptor-expressing tumors (i.e., breast and prostate), we have prepared by solid-phase peptide synthesis, a novel BN analog derived from the universal sequence of BN and conjugated to a widely characterized antineoplastic agent, methotrexate (MTX). MTX-BN, after radiolabeling with {sup 99m}Tc via stannous-tartrate exchange, showed a good stability against cysteine and histidine transchelation as well as a high in vitro metabolic stability in human plasma. In vitro cell-binding and internalization on MDA-MB-231, MCF-7, T47-D breast cancer and PC-3 prostate cancer cell lines demonstrated high affinity and specificity of {sup 99m}Tc-MTX-BN towards both human breast and prostate cancer cells (binding affinities in nanomolar range). In addition, the radioconjugate displayed a significant internalization (values ranged between 19-35%) into the tumor cells. In vivo biodistribution and clearance kinetics in Balb/c mice are characterized by an efficient clearance from the blood and excretion mainly through the renal-urinary pathway with some elimination via the hepatobiliary system. In vivo tumor uptake in nude mice bearing MDA-MB-231 cells was 2.70{+-}0.44% ID/g at 1 h, whereas in nude mice with human epidermoid KB cells the accumulation in the tumor was found to be 1.48{+-}0.31% ID/g at 1 h post injection. The tumor uptake was always higher than in the blood and muscle, with good tumor retention and good tumor-to-blood and tumor-to-muscle ratios. The accumulation/retention in the major organs (i.e., lungs, stomach, liver, intestines, etc.) was low to moderate (<6% ID/g) in both healthy and tumor-bearing mice. However, the uptake/retention in the kidneys was rather high (up to 11.05{+-}1.80% ID/g), which is of a

  18. Use of 99mTc-HMPAO-leucocyte scintigraphy coupled to 99mTc-colloid scintigraphy in diagnosing the osteo-articular sepsis

    International Nuclear Information System (INIS)

    The object of this study was the scintigraphic diagnosis of osseous sepsis based on the absence of congruence between leucocytic and colloidal fixations and the evaluation of the effect of coupling between the scintigraphies (sc) with leucocytes (A) and colloids (B) on the diagnosis feasibility index of this technique. Seventy four patients, 97 sc were divided into two groups according to the scintigraphic technique [(A) only]: 35 sc; [(A) and (B)]: 62 sc. [Prosthesis: 48 patients, 68 sc. Osteitis: 26 patients, 29 sc]. The scintigraphies were performed as follows: (A) autologous leucocytes labelled by 250 - 300 MBq of 99mTc-HMPAO, with planar incidences (15 min), 3 - 4 hours after injection and (B) Labelled nano-colloids labelled by 180 - 200 MBq of 99mTc, the same incidences, 30 min after injection. The delay between (A) and (B): 48 hours. The final diagnosis of sepsis: bacteriological and histologic criteria after biopsies and/or clinical, biological and radiological evolution. The comparison between the two groups is given in a table in terms of prevalence, sensitivity and specificity, using the χ2 and Fisher tests. In conclusion, the scintigraphy by 99mTc-HMPAO-leucocyte is a sensitive method (Se ∼ 90%) for the diagnosis of osseous sepsis and coupled to the scintigraphy by 99mTc-colloids allow obtaining a high specificity (Sp ≥ 90%) by reducing the number of falsely positive diagnoses

  19. The relationship between the percentage of lung shunting on Tc-99m macroaggregated albumin (Tc-99m MAA scan and the grade of hepatocellular carcinoma vascularity

    Directory of Open Access Journals (Sweden)

    Rania Refaat

    2014-06-01

    Conclusion: Tc-99m MAA scan is fundamental prior to Y-90 microsphere SIRT as it minimizes the risk of post-radioembolization complications, hence, enhancing the safety of Y-90 microsphere subsequent administration. Moreover, the percentage of lung shunting varies considerably among patients with HCC relying on the grade of tumor vascularity.

  20. Validity of dual tracer 99mTc-tetrofosmin and 99mTc-pertechnetate subtraction parathyroid scintigraphy in patients with primary and secondary hyperparathyroidism

    Directory of Open Access Journals (Sweden)

    Dugonjić Sanja

    2009-01-01

    Full Text Available Background/Aim. Primary hyperparathyroidism (pHPT is an endocrine disease with the third highest incidence of all endocrine disorders after diabetes mellitus and hyperthyroidism. pHPT is typically caused by a solitary parathyroid adenoma, less frequently by multiple parathyroid gland disease (MGD and rarely by parathyroid carcinoma. Secondary hyperparatyroidism (sHPT is a common complication in patients with chronic renal failure. The aim of this study was to estimate sensitivity of dual tracer 99mTctetrofosmin and 99mTc-pertechnetate subtraction scintigraphy in detection of abnormal parathyroid glands in patients with pHPT and sHPT confirmed by histopathology. Methods. In 46 patients, (77 abnormal parathyroid glands, 30 with pHPT and 16 with sHPT parathyroid scintigraphy was done preoperatively. All the patients had histopathological confirmation of diagnosis. Abnormal parathyroid glands weighted from 0.1 to 7 g. After iv injection dynamic scintigraphy during 25 minutes (one frame-one minute using 555 MBq of 99mTc-tetrofosmin, and three hours latter using 111 MBq of 99mTc /pertechnetate was performed. 99mTc-tetrofosmin dynamic study was followed by static scintigraphy of the neck and chest 30 minutes, 1, 2 and 3 hours after iv injection. Results. An abnormal scintigraphic finding was found in 44 of 46 patients with sensitivity of 96%. In pHPT sensitivity was 93% (28 of 30 patients, and 28 of 30 glands. In sHPT scintigraphy was abnormal in all the patients (sensitivity 100%. In the patients with sHPT scintigraphy detected 30 of 47 abnormal parathyroid glands (sensitivity 64%. An overall sensitivity of scintigraphy per gland, for pHPT and sHPT in detecting 58 of 77 abnormal parathyroid glands was 75%. Conclusion. An abnormal scintigrafic result per patient was found in 44 patients (sensitivity 96% and 58 of 77 abnormal parathyroid glands were detected (sensitivity 75%. A high sensitivity of dual tracer subtraction 99mTc-tetrofosmin/99m

  1. 肺肿瘤99mTc-MDP显像研究%Study of 99mTc-MDP Imaging in Diagnosis of Lung Neoplasms

    Institute of Scientific and Technical Information of China (English)

    谢昌辉; 马志海; 李秀江; 张玉萍; 高凤彤

    2004-01-01

    目的探讨99mTc-羟基亚甲基二磷酸(MDP)显像鉴别诊断肺肿瘤的机制和临床价值.方法对63例经病理证实的肺肿瘤患者和10例正常对照进行早期10min99mTc-MDP显像,用感兴趣区分析法计算肿瘤和正常肺组织的放射性比(T/NT),探讨肺肿瘤病灶早期摄取99mTc-MDP的机制.结果肺癌的T/NT值(2.36±0.56)明显高于良性病灶(1.44±0.47)和正常对照(0.98±0.12)(t=5.26,7.70,P<0.01);以摄取比值>1.8为判断标准,99mTc-MDP显像诊断肺癌的灵敏度、特异性和准确率分别为88.2%、86.3%和86.7%;肺癌组织内残留的宿主血管、大量的新生血管和病变周围的血管集束为高水平99mTc-MDP血流灌注的特征性病理结构,肺癌的局部浸润转移促进了99mTc-MDP的摄取.结论99mTc-MDP的早期摄取与肺肿瘤的血供和局部浸润转移密切相关,对肺肿瘤的鉴别诊断有一定的临床价值.

  2. 99mTc-MIBI进行鼻咽癌显像的临床研究%Detection of nasopharyngeal carcinoma with Tc-99m MIBI

    Institute of Scientific and Technical Information of China (English)

    杜建强; 裴庆华; 岳殿超; 曾世荃

    2004-01-01

    Objective It is difficult to differentiate primary and recurrent nasopharyngeal carcinoma (NPC). Using 99mTc MIBI scintigraphy, we study patients with NPC in order to determine the efticacy of 99mTc MIBI scintigraphy in detecting the primary, recurrent and metastatic tumors. Method NPC patients were divided into two groups, primary (n= 43) and recurrent (n= 17). 99mTc MIBI SPECT study of head and neck and whole body scan to detect the primary, recurrent and metastases foci were performed on both groups as well as on volunteers (n = 21). Comparing with the scalp, the uptakes on the nasopharynx were divided into 5 grades, assessed by 3 physicians independently without any clinical information. Results In the primary NPC group, 36 patients were positive and 5 were negative. 13 patients had metastases in neck lymph nodes and 2 had lungs metastases. The specificity was 90.5 % ( 19 of 21) and the sensitivity was 82.7 % (36 of 43 ). In the recurrent group, 13 patients were positive, and 4 were negative. The sensitivity was 76.5 % ( 13 of 17 ). Conclusion This study suggests that 99mTc MIBI SPECT is a useful method for detecting NPC with particular potential value in diagnosis of recurrent NPC.%目的在临床上,鼻咽癌复发的诊断是非常困难的,我们用99mTc-MIBI来探索鼻咽癌的原发灶、复发灶和转移灶的诊断.方法用99mTc-MIBI断层显像检测21例对照组和41例治疗前鼻咽癌病例,病灶的放射性摄取与头皮比较,由3名以上的医师在不知道任何临床情况下分别阅片.结果41例治疗前鼻咽癌病例中共有36例阳性,特异性为90.5%,灵敏度为83.7%.结论99mTc-MIBI显像对鼻咽癌原发灶和转移灶的诊断,是一种灵敏的方法,值得进一步探讨.

  3. Human pharmacokinetics, biodistribution and dosimetry of the kit of monoclonal antibody IOR EGF/R3 labelled with {sup 99m} Tc

    Energy Technology Data Exchange (ETDEWEB)

    Torres, L.A.; Ramos, M.; Perera, A.; Hernandez, A.; Iznaga, M.E. N. [Solano, Ivette Alvarez, Jose L. Rodriguez. Centro de InvestigacionesClinicas. 34 no.4501 e/45 y 47 Kohly, Playa, C. Habana (Cuba)

    1998-12-31

    The aim of this work was to assess the human pharmacokinetics, biodistribution and dosimetry of the {sup 99m} Tc-labeled MAb ior egf/r3. Five patients were included in the biodistribution and dosimetric studies and three in the pharmacokinetic analysis. Multiple blood and urine samples we recollected and sequential anterior and posterior whole-body scintigraphies u pto 24 hr post-injection were performed to all patients . The internal radiation dosimetry was estimated from gamma camera imaging data using the methods developed by the Medical Internal radiation dosimetry (MIRD)committee. Raw data were computed from operations between gamma graphic images and regions of interest (ROI) using the Bio-Dose software and time-activity curves were calculated in order to determine the residence times of the source organs. The Pharmacokinetics and Biodistribution results showed that this compound have a bio exponential plasmatic and blood clearance with a rapid biodistribution phase of 9.1 {+-} 8.4 min and 12.2{+-}4.4 min, respectively, and a slower elimination phase of 6.6 {+-} 1.6 hr and 10.8 {+-} 6.8 hr. respectively. The urinary and hepatobiliary excretion showed 4.7 {+-} 0.4 % and 9.9 {+-} 1.8 % of the total administered dose,eliminated by these ways. Liver was the target organ of this product and had an uptake peak at 1 hr post-injection (61.2%) and a great retention of the MAb(T 1/2 eff = 5.3 hr, T 1/2 Biol. = 45.0 hr). The dosimetric results showed that liver, gallbladder and spleen received the higher absorbed. The effective dose and the effective equivalent dose were 1,2E-01 mSv/MBq and 9,2E-02 mSv/MBq respectively. These results allow to see the i or egf/r3 kit in a safe and controlled way. (Author)

  4. Use of {sup 99m}Tc-mercaptoacetyltriglycine (MAG3)-biocytin hepatobiliary scintigraphy to study the protective effect of a synthetic enzyme inhibitor on acute hepatotoxicity in mice

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Meyoung-kon; Song, Byoung J.; Seidel, Juergen; Soh, Yunjo; Jeong, Kyu-Shik; Kim, In Sook; Kobayashi, Hisataka; Green, Michael V.; Carrasquillo, Jorge A.; Paik, Chang H. E-mail: paik@nmdhst.cc.nih.gov

    1998-08-01

    Recent data suggest that inhibitors of ethanol-inducible cytochrome P450 (CYP2E1) can protect the liver from injury caused by various substrates of CYP2E1. In this study, we measured the protective effect of isopropyl-2-(1,3-dithioetane-2-ylidene)-2[N-(4-methylthiazol-2-yl) -carbamoyl]acetate (YH439), a transcriptional inhibitor of CYP2E1, against carbon tetrachloride (CCl{sub 4})-induced hepatotoxicity by using various conventional methods and dynamic scintigraphy with {sup 99m}Tc-mercaptoacetyltriglycine (MAG3)-biocytin, a recently developed scintigraphic agent. Balb/c mice were pretreated with two doses of YH439 (50 or 150 mg/kg per day) at 48 h and 24 h and one dose of CCl{sub 4} (0.25 mL/kg) at 18 h before scintigraphy. The results were compared with those of two other groups, one that received CCl{sub 4} but not YH439, and the other that received neither (control). Scintigraphic images were acquired continuously at 15-sec intervals for 30 min. Pharmacokinetic parameters, such as peak liver/heart ratio (r{sub max}), peak liver uptake time (t{sub max}), and hepatic half-clearance time (HCT), were obtained from time-activity curves derived from regions-of-interest (ROI) over the liver and the heart. Acute administration of CCl{sub 4} alone caused centrilobular necrosis and serum transaminase levels to rise more than 5 times higher than those of the control group. Pharmacokinetic parameters also changed significantly from those of the control group. Administration of YH439 prevented centrilobular necrosis and significantly improved pharmacokinetic parameters. This study demonstrates for the first time that hepatobiliary scintigraphy can be used to study in vivo biochemistry of the CYP2E1 inhibitor (YH439) against liver toxicity.

  5. Use of 99mTc-mercaptoacetyltriglycine (MAG3)-biocytin hepatobiliary scintigraphy to study the protective effect of a synthetic enzyme inhibitor on acute hepatotoxicity in mice.

    Science.gov (United States)

    Kim, M K; Song, B J; Seidel, J; Soh, Y; Jeong, K S; Kim, I S; Kobayashi, H; Green, M V; Carrasquillo, J A; Paik, C H

    1998-08-01

    Recent data suggest that inhibitors of ethanol-inducible cytochrome P450 (CYP2E1) can protect the liver from injury caused by various substrates of CYP2E1. In this study, we measured the protective effect of isopropyl-2-(1,3-dithioetane-2-ylidene)-2[N-(4-methylthiazol -2-yl)-carbamoyl]acetate (YH439), a transcriptional inhibitor of CYP2E1, against carbon tetrachloride (CCl4)-induced hepatotoxicity by using various conventional methods and dynamic scintigraphy with 99mTc-mercaptoacetyltriglycine (MAG3)-biocytin, a recently developed scintigraphic agent. Balb/c mice were pretreated with two doses of YH439 (50 or 150 mg/kg per day) at 48 h and 24 h and one dose of CCl4 (0.25 mL/kg) at 18 h before scintigraphy. The results were compared with those of two other groups, one that received CCl4 but not YH439, and the other that received neither (control). Scintigraphic images were acquired continuously at 15-sec intervals for 30 min. Pharmacokinetic parameters, such as peak liver/heart ratio (r(max)), peak liver uptake time (t(max)), and hepatic half-clearance time (HCT), were obtained from time-activity curves derived from regions-of-interest (ROI) over the liver and the heart. Acute administration of CCl4 alone caused centrilobular necrosis and serum transaminase levels to rise more than 5 times higher than those of the control group. Pharmacokinetic parameters also changed significantly from those of the control group. Administration of YH439 prevented centrilobular necrosis and significantly improved pharmacokinetic parameters. This study demonstrates for the first time that hepatobiliary scintigraphy can be used to study in vivo biochemistry of the CYP2E1 inhibitor (YH439) against liver toxicity. PMID:9751424

  6. Clinical evaluation of 99mTc-Technegas in pulmonary ventilation study

    International Nuclear Information System (INIS)

    To evaluate the clinical usefulness and the possibility of using 99mTc-Technegas (99mTc), the pulmonary distribution of 99mTc was assessed in 40 patients with various pulmonary diseases and compared with 81mKr gas (81mKr) and 99mTc-MAA. In 24 patients, the 99mTc was inhaled several times during deep inspiration with a breath hold in the supine position. In 16 patients, ventilation images were acquired during tidal breathing or deeper breathing without a breath hold. In all patients, a continuous inhalation of 81mKr was made after the inhalation of 99mTc. Then 99mTc-MAA was administered intravenously. Histograms of the frequency distribution of the ventilation-perfusion (V/Q) ratio for anterior, posterior and superimposed images were constructed with the obtained ventilation images of 99mTc or 81mKr and perfusion images of 99mTc-MAA. After the region of interest had been set to include the entire lungs, the V/Q ratio for each pixel in the region was calculated using a 128 x 128 matrix. In a comparative study with 81mKr, 99mTc showed a similar distribution to that of 81mKr in most patients, although some patients with obstructive disease showed small region of high-count density in the lung. 99mTc-SPECT showed the increased basal deposition that was seen at the back of lung in 8 of 9 patients with breath hold and in 8 of 13 patients without breath hold. This deposition difference was considered to be caused by the difference in inhalation method and the effect of gravity. In comparison of the proportion of V/Q counts, there was a significant positive correlation between 99mTc and 81mKr in breath hold patients for anterior images, posterior images, and superimposed images. In 16 patients without breath hold, a correlation was acquired as well as using breath hold for anterior images, posterior images, and superimposed images. (K.H.)

  7. Synthesis, biodistribution and imaging of {sup 99m}tc-7-HYNIC-TAXOL

    Energy Technology Data Exchange (ETDEWEB)

    Rha, D. S.; Park, J. Y.; Awh, O. D. [College of Health Science, Yonsei University, Seoul (Korea, Republic of); Lee, T. S.; Choi, T. H.; Woo, K. S.; Chung, W. S.; Choi, C. W.; Kim, S. M. [Korea Institutes of Radiological and Medical Sciences, Seoul (Korea, Republic of)

    2005-07-01

    Taxol has been used in the treatment of breast, ovary and lung cancers. To evaluate the feasibility of 99mTc-7-HYNIC(hydrazino nicotinamide)-taxol as a tumor imaging agent, it was synthesized, and its biodistribution and gamma camera image were obtained in B16-F10 melanoma bearing C57BL6 mice 7-t-BOC-HYNIC-taxol was synthesized through six steps, and 7-HYNIC-taxol was finally obtained by t-BOC deprotecting from 7-t-BOC-HYNIC-taxol. The product was purified by column chromatography. 99mTc-7-HYNIC-taxol complex from 7-HYNIC-taxol was prepared by labeling with 99mTc in the presence of SnCl2{center_dot}2H20 and tricine. The biochemical behaviors of the complex such as in vitro stability and lipophilicity, in vitro transchelation were investigated. The biodistribution and in vivo image of 99mTc-7-HYNIC-taxol were obtained in B16-F10 melanoma bearing C57BL6 mice. After 1, 6 and 24 hr post-injection, the weight and radioactivity of each organ were measured and gamma camera image was obtained. The total synthetic yield of 7-HYNIC-taxol was 42.6%. Radiolabeling yield of 99mTc-HYNIC-taxol was 99.9%. 99mTc-7-HYNIC-taxol was stable at 37? for 24 hrs. 99mTc-7-HYNIC-taxol was slightly more soluble in water than in organic solvent. The binding ability of 99mTc-7-HYNIC-taxol to serum proteins was 39.9%. In vivo transchelation test, the 99mTc-7-HYNIC-taxol retained over 86% of radiochemical purity after incubation with DTPA or cysteine. 99mTc-7-HYNIC-taxol was intravenously administered to C57BL6 mice bearing B16-F10 melanoma at footpad. Tumor/blood ratios were 1.17, 26.0, and 2.87, and tumor/muscle ratios were 12.2, 168, and 15.0 at 1 h. 6 h and 24 h post injection, respectively. The gamma camera image was obtained at 6 h post injection showed selectively localized in tumor. 99mTc-7-HYNIC-taxol showed high stability and was selectively localized in B16-F10 melanoma. These results suggest that 99mTc-7-HYNIC-taxol can be used as tumor imaging agent.

  8. The trend of 99mTc generator in FNCA countries

    International Nuclear Information System (INIS)

    In the 2001 Workshop on Utilization of Research Reactors held in Beijing, eight delegates from the FNCA countries, namely China, Indonesia, Japan, Korea, Malaysia, the Philippines, Thailand and Vietnam, for the 99mTc Generator Project, presented papers or orally pleaded the current status of utilization and production in terms of 99Mo solution, 99Mo/99mTc generator or extracted 99mTc solution in each country. This paper is a brief compilation of these topics and some additional information obtained afterward the workshop. (author)

  9. 99mTc-ciprofloxacin imaging in infectious and sterile inflammation in rat model

    International Nuclear Information System (INIS)

    99mTc-Ciprofloxacin is a potentially specific agent for bacterial infection. The aim of this study was to find out whether 99mTc-Ciprofloxacin accumulation can differentiate bacterial infectious inflammation from nonbacterial sterile inflammation. 99mTc-Ciprofloxacin was synthesized with 99mTc 20 mCi, ciprofloxacine 2 mg, formamidine sulphinic acid 1 mg, and 15 sec heating in microwave. For induction of infectious or sterile inflammation in SD rats, 2 x 108 of S. aureus in 0.2ml (group1, n=9) or 0.2 ml of terpentine oil (group 2, n=10) were injected to thigh muscle. Three days later, 99mTc-Ciprofloxacin images were obtained at 4 hrs after iv injection of 37MBq of 99mTc-Ciprofloxacin. Immediately after imaging, rats were sacrificed and dissected to obtain %ID/g of normal organs and inflammatory lesions. For histopathologic evaluation, tissue specimens from the inflammatory lesions were obtained. The induction of infection/inflammation produced marked swelling of thighs in both groups. 99mTc-Ciprofloxacin imaging of both groups showed increased uptake, but target to background uptake ratio in group 1 was significantly higher than group 2 (3.70±0.5 vs 2.18±0.3, p99mTc-Ciprofloxacin in liver, slpeen, kidney were 6.1±0.45, 5.1±0.42, 3.42±0.2%ID/g, respectively, and inflammatory lesion in group 1 and group 2 were 0.42±0.09 and 0.24±0.02%ID/g, respectively. Both lesion to normal muscle activity ratio and lesion to blood activity ratio of 99mTc-Ciprofloxacin in group 1 were significantly higher than group 2 (5.29±2.3 vs 1.69±0.2, 1.45±0.47 vs 0.93±0.2, p99mTc-Ciprofloxacin uptake is significantly higher in bacterial infectious inflammation than sterile inflammation. However, further studies and cautious clinical application are needed for differentiating infectious from sterile inflammation using 99mTc-Ciprofloxacin imaging, because there is still significant degree of 99mTc-Ciprofloxacin accumulation in sterile inflammation

  10. Preparation of 99mTc Radiopharmaceuticals for Diagnosis of Bacterial Infection

    International Nuclear Information System (INIS)

    Full text: Preparation of 99mTc-ciprofloxacin by Radioisotope Center, Thailand Institute of Nuclear Technology, for diagnosis of bacterial infection proceeded by varying the factors which affected this compound. The resulted 99mTc-ciprofloxacin had a pH of 4.0 - 5.0, 0.25 ≅ 0.56 % injected dose per gram of infected area (1 and 3 hours after injection) and at least 9 5% radiochemical purity with 6-hour stability. 99mTc-ciprofloxacin was found sterile, pyrogen-free and non-toxic

  11. Leukocyte-technetium-99m uptake in Crohn s disease:Does it show subclinical disease?

    Institute of Scientific and Technical Information of China (English)

    Luciene; G; Mota; Luiz; GV; Coelho; Carlos; JR; Simal; Maria; LA; Ferrari; Clodomiro; Toledo; Josep; Martin-Comin; Simone; OF; Diniz; Valbert; N; Cardoso

    2010-01-01

    AIM:To evaluate inflammatory activity in patients with Crohn's disease (CD) using technetium-99m-hexamethylpropyleneamine oxime (99mTc-HMPAO) granulocyte scintigraphy.METHODS: Twenty patients (7 male and 13 female) with CD and five healthy volunteers were selected for 99mTc-HMPAO granulocyte scintigraphy. The Crohn's Disease Activity Index (CDAI), blood tests and C-reactive protein (CRP) of each patient were performed 7 d before the scintigraphic images. The leukocytes were labeled according to the Internat...

  12. Sup(99m)Tc(Sn)-sucralfate; development of an instant labelling kit

    International Nuclear Information System (INIS)

    An instant labelling kit for the preparation of 99mTc(Sn)-sucralfate has been developed containing 500 mg sucralfate and 1 mg SnCl2 · 2 H2O. The labelling yield, in vitro stability and biodistribution indicate (bio)equivalence of 99mTc(Sn)-sucralfate and 99mTc(HSA)-sucralfate. The percentages of labelling obtained with the kit during a period of 8 months always exceeded 95%. The preparation kit is inexpensive and the labelling procedure fast and easy. (author)

  13. The role of Tc-99m RBC scintigraphy in the differential diagnosis of orbital cavernous hemangioma.

    Science.gov (United States)

    Sayit, E; Durak, I; Capakaya, G; Yilmaz, M; Durak, H

    2001-04-01

    The cavernous hemangioma is the most common benign orbital tumor in adults. Its presentation is during the forth to fifth decades with a slowly progressive unilateral proptosis. Intraconal cavernous hemangiomas may be difficult to differentiate from other intraconal lesions such as schwannomas, meningiomas and hemangiopericytomas. We report a case of orbital cavernous hemangioma diagnosed by Tc-99m RBC scintigraphy. Tc-99m RBC scintigraphy revealed a typical scintigraphic pattern in which there is intense focally increased uptake on the delayed image. We conclude that Tc-99m RBC scintigraphy can be a useful method in the differential diagnosis of orbital cavernous hemangioma as in hepatic hemangioma. PMID:11448074

  14. A conveniently prepared Tc-99m resin for semisolid gastric emptying studies

    Energy Technology Data Exchange (ETDEWEB)

    Wirth, N.; Swanson, D.; Shapiro, B.; Nakajo, M.; Coffey, J.L.; Eckhauser, F.; Owyang, C.

    1983-06-01

    A polystyrene resin, suitable for semi-solid gastric emptying studies, was rapidly (less than 20 min) and conveniently prepared using commercially available reagents. Using the outlined procedure, Chelex-100 resin bound Tc-99m with greater than 98% labeling efficiency. The resulting Tc-99m Chelex-100 resin demonstrated excellent in vitro and in vivo stability. The clinical application of Tc-99m Chelex-100 resin, mixed with oatmeal, was tested in normal subjects and in various patient groups, including diabetic autonomic neuropathy, pyloric obstruction, postoperative dumping syndrome, and morbidly obese patients before and after gastroplasty.

  15. Conveniently prepared Tc-99m resin for semisolid gastric emptying studies

    Energy Technology Data Exchange (ETDEWEB)

    Wirth, N. (Univ. of Michigan, Ann Arbor); Shapiro, B.; Nakajo, M.; Coffey, J.L.; Eckhauser, F.; Owyang, C.

    1983-06-01

    A polystyrene resin, suitable for semi-solid gastric emptying studies, was rapidly (<20 min) and coveniently prepared using commercially available reagents. Using the outlined procedure, Chelex-100 resin bound Tc-99m with greater than 98% labeling efficiency. The resulting Tc-99m Chelex-100 resin demonstrated excellent in vitro and in vivo stability. The clinical application of Tc-99m Chelex-100 resin, mixed with oatmeal, was tested in normal subjects and in various patient groups, including diabetic autonomic neuropathy, pyloric obstruction, postoperative dumping syndrome, and morbidly obese patients before and after gastroplasty.

  16. sup(99m)Tc-pyrophosphate and 201Tl myocardial scintigrams in a patient with myocarditis

    International Nuclear Information System (INIS)

    Myocardial necrosis in acute myocarditis was investigated by scintigraphy. sup(99m)Tc-pyrophosphate (PYP) and 201TI myocardial scintigrams were obtained on a patient with acute myocarditis due to mycoplasma infection. sup(99m)Tc-PYP myocardial scintigrams in the acute stage demonstrated grade 2+ findings, which remained until the chronic stage. 201TI myocardial scintigrams in the acute stage revealed impaired perfusion restricted to the posterolateral wall, and this decrease continued through the chronic stage. It was concluded that both of sup(99m)Tc-PYP and 201TI myocardial scintigrams can reveal abnormality of acute myocarditis. (Ueda, J.)

  17. Synthesis and characterization of zirconium molybdates of 99 Mo/99m Tc generators

    International Nuclear Information System (INIS)

    The zirconium molybdates are gels which are used as cation exchangers in the production of 99 Mo/99m Tc generators. The synthesis method and the characterization of these gels by thermogravimetry, infrared spectroscopy and X-ray diffraction is presented with the purpose of finding which the factors are that influence in the efficiency of the 99m Tc production. The results show that the quantity of molecular water contained in gel, is possibly the cause of variations of the efficiencies of the 99 Mo/99m Tc generator. (Author)

  18. Lyophilized kits of diamino dithiol compounds for labelling with {sup 99m}-technetium. Pharmacokinetics studies and distribution compartmental models of the related complexes; Conjuntos de reativos liofilizados de compostos diaminoditiolicos para marcacao com tecnecio-99m. Estudo farmacocinetico e elaboracao de modelos compartimentalizados dos respectivos complexos

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Elaine Bortoleti de

    1995-07-01

    The present work reflects the clinical interest for labelling diamino dithiol compounds with technetium-99m. Both chosen compounds, L,L-Ethylene dicysteine (L,L-EC) and L,L-Ethylene dicysteine diethyl esther (L,L-ECD) were obtained with relative good yield and characterized by IR and NMR. The study of labelling conditions with technetium-99m showed the influence of the type and mass of reducing agent as well as the pH on the formation of complexes with desired biological characteristics. Radiochemical purity was determined by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Lyophilised kits of L,L-EC and L,L-ECD for labelling with {sup 99m}Tc were obtained, with stability superior to 120 days, when stored under refrigeration, enabling the kits marketing. The ideal formulation of the kits as well as the use of liquid nitrogen in the freezing process, determined the lyophilization success. Distribution biological studies of the {sup 99m}Tc complexes were performed on mice by invasive method and on bigger animals by scintigraphic evaluation. Biological distribution studies of the complex {sup 99m}Tc-L,L-EC showed fast blood clearance, with the elimination of about 90% of the administered dose after 60 minutes, almost exclusively by the urinary system. The biological distribution results were adjusted to a three compartmental distribution model, as expected for a radiopharmaceutical designed to renal dynamic studies, with tubular elimination. The complex interaction with renal tubular receptors is related with structural characteristics of the compound, more specifically with the presence and location of polar groups. In comparison with {sup 99m}Tc-L,L-EC, biological studies of the complex {sup 99m}Tc -L,L-ECD showed different distribution aspects, despite some structural similarities. The presence of ethyl groups confers to the complex neutrality and lipophilicity. It cross the intact blood brain barrier and is retained in the brain

  19. Imaging of dopamine transporters in humans with technetium-99m TRODAT-1

    International Nuclear Information System (INIS)

    Technetium-99m TRODAT-1, a tropane derivative, has shown promise as a tracer for the imaging of dopamine transporters in preliminary studies in rats and baboons. The present report concerns the first study of the use of [99mTc]TRODAT-1 for the same purpose in humans. The specific uptake of [99mTc]TRODAT-1 in dopamine transporter sites located in the basal ganglia area was confirmed: the best contrast between the basal ganglia and the occipital area, which is devoid of dopamine transporters, was achieved at 120-140 min following injection. The development of a 99mTc-based agent bypasses the need for cyclotron-produced radionuclides, which will be of benefit for routine clinical studies. (orig.). With 2 figs

  20. Use of /sup 99m/Tc-labelled chelates to evaluate their penetration into the dentin

    International Nuclear Information System (INIS)

    The /sup 99m/Tc-labelled EDTA and DPTA complexes proved to be of high purity. After application of the complexes into the root canal their penetration into the dentin was examined autoradiographically

  1. Labelling, biodistribution and compartmental analysis of N-acetylcysteine labelled with Tc-99m. Comparative investigation with with {sup 99m} Tc-MIBI in an in vivo tumoral model; Estudo de marcacao, biodistribuicao e analise compartimental da N-acetil cisteina marcada com Tc-99m. Investigacao comparativa com MIBI-{sup 99m}Tc em modelo tumoral in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Faintuch, Bluma Linkowski

    1997-07-01

    Labelling and biodistribution studies were done with two different ligands, respectively Methoxy isobutyl isonitrile (MIBI) and N-acetylcysteine (NAC), employing Tc-99m as a tracer. The main objective was to assess the pharmacokinetic properties of the second substance, aiming at its possible application in cancer diagnosis. To this purpose an in vivo investigation was done using healthy and tumor-bearing rats with experimental cancer. Images of tumor-bearing rats registered in a scintillation camera indicated that with {sup 99m} Tc-MIBI none of the two selected times was adequate for visualization of the cancer mass. In contrast, {sup 99m} Tc-NAC permitted clear identification of the humor, four hours after injection. The results have demonstrated that {sup 99m} Tc-NAC is a radiopharmaceutical with affinity for cancer tissue and promising for further investigation concerning imaging diagnosis of tumors. (author)

  2. .sup.100Mo compounds as accelerator targets for production of .sup.99mTc

    Energy Technology Data Exchange (ETDEWEB)

    Richards, Vernal; Lapi, Suzanne

    2016-09-20

    Methods of synthesizing .sup.100Mo.sub.2C and .sup.99mTcO.sub.4.sup.- are disclosed. Methods of .sup.100Mo.sub.2C generation involve thermally carburizing .sup.100MoO.sub.3. Methods of .sup.99mTcO.sub.4 generation involve proton bombardment of .sup.100Mo.sub.2C in a cyclotron. Yields of .sup.99mTcO.sub.4 can be increased by sintering .sup.100Mo.sub.2C prior to bombardment. The methods also include recycling of .sup.100Mo.sub.2C to form .sup.100MoO.sub.3. SPECT images obtained using .sup.99mTcO.sub.4 generated by the disclosed methods are also presented.

  3. A kit for the preparation of basic sup(99m)Tc penicillamine for renal scanning

    International Nuclear Information System (INIS)

    A kit for preparing basic (pH 8.4) sup(99m)Tc-penicillamine complex for renal studies is described. The radiopharmaceutical prepared from the kit localizes in the kidneys, primarily in the renal cortex. Data is presented which demonstrates that the kit method for sup(99m)Tc-penicillamine results in biological distribution of the sup(99m)Tc-penicillamine complex equivalent to that observed for the older extemporaneous method of preparation. The per cent of inject dose localized in the kidneys of rabbits at one hour is 18.3 +- 3.1%, which compares favorably with other sup(99m)Tc-complexes used for renal imaging. (author)

  4. Incidentally detected diaphragmatic hernia mimicking angina pectoris with Tc-99m MIBI myocardial perfusion imaging

    Science.gov (United States)

    Cınar, Alev; Sadıc, Murat; Demırel, Koray; Korkmaz, Meliha

    2013-01-01

    In recent years 99mTechnetium methoxy-isobutyl-isonitrile (99mTc MIBI) is widely used in the evaluation of myocardial perfusion imaging (MPI). In this imaging protocol besides the cardiac evaluation, numerous other organs are included in the field of view. 99mTechnetium MIBI is taken up in all metabolically active tissue in the body except for the brain. Extracardiac uptake patterns as benign or serious conditions can be revealed during the MPI. In the imaging protocol, we should be aware of distribution of this radiotracer in order to recognize the abnormal uptake. Here in, we present a large diaphragmatic hernia, seemed as a mirror vision of myocardium that was determined incidentally during the MPI with 99mTc MIBI. PMID:24379540

  5. Calibration of KRISS reference ionization chamber for key comparison of (99m)Tc measurement.

    Science.gov (United States)

    Lee, Jong-Man; Lee, K B; Lee, S H; Park, Tae Soon

    2012-09-01

    KRISS, as the national metrology institute of Korea, has used a reference ionization chamber system to certify the activity of (99m)Tc aqueous sources, but could only recently participate in a comparison exercise by the BIPM (BIPM.RI(II)-K4.Tc-99m) to secure the international equivalence of (99m)Tc radioactivity measurement by way of the BIPM transfer instrument (SIRTI). The KRISS ionization chamber system was calibrated about 100 days before the comparison with a (99m)Tc solution source standardized by the 4πβ(LS)-γ(NaI(Tl)) coincidence counting method. During the comparison, beginning with a higher activity mother solution, the KRISS ionization chamber measured its specific activity without a dilution. The activity of a diluted-solution source was measured by the SIRTI at the same time.

  6. Patterns of localization of sup(99m)Tc-diphosphonate and tetracycline in bone tumors

    International Nuclear Information System (INIS)

    In the beginning of a study on mechanism of sup(99m)Tc-phosphoric acid compounds accumulation, regions where sup(99m)Tc-methylene diphosphonate (MDP) or tetracycline accumulated were compared in 5 patients with primary bone tumors (one with osteosarcoma, one with malignant fibrous histocytoma, one with osteochondroma, and two with chondrosarcoma). In accordance with regions where tetracycline accumulated, much sup(99m)Tc-MDP accumulated in neoplastic bone, calcified and ossified cartilage, and reactive new bone around bone tumors. It was concluded from above-mentioned results and mechanism of tetracycline accumulation that much sup(99m)Tc-MDP penetrated well and accumulated in reactive new bone around bone tumors probably because in reactive new bone around bone tumors, the size of hydroxyapatite was small, the density of matrix of bone was low, and surface area of hydroxyapatite crystal was very wide. (Tsunoda, M.)

  7. Normal appearances of technetium-99m dimercaptosuccinic acid in children on planar imaging

    Energy Technology Data Exchange (ETDEWEB)

    Pusuwan, P.; Reyes, L.; Gordon, I. [Department of Radiology, Hospital for Children NHS Trust, London (United Kingdom)

    1999-05-01

    Thirty-two children who underwent renal arteriography with normal results also underwent technetium-99m dimercaptosuccinic acid (DMSA) imaging. Variations in the normal appearance of {sup 99m}Tc-DMSA images are described in these children. Criteria for high-quality {sup 99m}Tc DMSA images are defined in terms of kidney outline and contrast between the inner and the outer part of the kidney. Most of the images in this study were of high quality. The two most common features of normal {sup 99m}Tc-DMSA images were a round-shaped (50%) or flat (either lateral or medial aspect) (24.8%) contour. A small number of unusual appearances were observed and these have been illustrated. The mean differential function of the left kidney was 51% with a range of 45%-57%. (orig.) With 9 figs., 3 tabs., 15 refs.

  8. Detection of Meckel's diverticulum in an infant by Tc-99m-sulfur colloid scintigraphy

    International Nuclear Information System (INIS)

    Active lower intestinal bleeding from a Meckel's diverticulum in an infant was detected with Tc-99m-sulfur colloid scintigraphy. This technique expands the scintigraphic armamentarium for evaluation of lower intestinal bleeding in infants and children

  9. Formulation and preliminary clinical application of the 99mTc(V)-dimercaptosuccinic acid ''instant'' kit

    International Nuclear Information System (INIS)

    99mTc labelling of DMSA under moderate reduction conditions (alkaline pH and low concentration of Sn(II)) leads to formation of various DMSA complexes. One of them 99mTc(V)-DMSa is proved to be suitable for tumour imaging in nuclear medicine diagnostics. The aims of our study were to define the optimum terms for high yield of 99mTc(V)-DMSA complex and kit formulation, which can be conveniently applied for diagnosis in nuclear medicine laboratories. The 99mTc labelled DMSA complexes were analysed by means of ascending thin layer chromatography (TCL) in three mobile phases - methyl ethyl ketone, n-butanol/acetic acid/water 3/2/3 and 0.154M NaCl. Appropriate formulae were chosen for kit preparation and applied for preliminary clinical investigations. (orig.)

  10. The preparation and clinical application of 99mTc-HSA instant kit

    International Nuclear Information System (INIS)

    The preparation and preliminary clinical application of 99mTc-HSA instant kit are reported. The data show that the radiochemical purity of 99mTc-HSA prepared by the method discribed is > 97%. Its stability is excellent in vitro and it can be left at room temperature for more than 2 hours. The T1/2 for disappearance of radio activity from the blood was 243 minutes. Sn-HSA kits can be stored at 2 ∼ 4 deg C for 3 months. By the use of 99mTc-HSA, the liver blood pool imaging and the gated cardiac blood pool imaging were performed satisfactoryly in 30 and 102 cases respectively. No toxic effects were observed in the patients examined. The authors are of the opinion that 99mTc-HSA prepared by the method is a successful agent for various blood pool imaging and is suitable for routine use

  11. Correlation of Tc-99m GSA hepatic studies with biopsies in patients with chronic active hepatitis.

    Science.gov (United States)

    Tomiguchi, S; Kira, T; Oyama, Y; Nabeshima, M; Nakashima, R; Tsuji, A; Kojima, A; Takahashi, M; Yoshimatsu, S; Sagara, K

    1995-08-01

    To determine whether scintigraphic findings of Tc-99m DTPA-galactosyl-HSA (GSA) correspond to histopathologic findings, Tc-99m GSA hepatic scintigraphy and biopsy were compared in 65 patients with chronic active hepatitis. After injecting 185 MBq of Tc-99m GSA, anterior images were obtained at 5 minutes and 15 minutes. Scintigrams were classified into three grades according to the extent of visualization of the cardiac blood pool on 5 minute and 15 minute images. Biopsies were subjectively graded for findings of necrosis and fibrosis. Scintigraphic grades on 5 minute images were correlated with hepatic necrosis and fibrosis and those on 15-minute images with hepatic fibrosis. Scintigraphic abnormalities of Tc-99m GSA correlated well with histopathologic abnormalities, especially with hepatic fibrosis and necrosis in patients with chronic active hepatitis. PMID:7586877

  12. The Noah's Ark experiment: species dependent biodistributions of cationic 99mTc complexes

    International Nuclear Information System (INIS)

    The time dependent biodistributions of three related 99mTc complexes of 1, 2-bis(dimethylphosphino)ethane (DMPE) were evaluated in several animal species including humans: trans-[99mTcv(DMPE)2O2]+, trans-[99mTcIII(DMPE)2Cl2]+ and [99mTcI(DMPE)3]+. Imaging studies were performed in 10 animal species to evaluate these complexes as myocardial perfusion imaging agents. Animal models adequately predict the uninteresting behaviour of the Tc(V) cation in humans, predict to only a very limited extent the behaviour of the Tc(III) cation in humans and totally fail to predict the behaviour of the Tc(I) cation in humans. (U.K.)

  13. The value of {sup 99m}technetium-MIBI scintiscanning for diagnostic evaluation of breast cancer; Stellenwert der {sup 99m}Technetium-MIBI-Szintigraphie in der Mammadiagnostik

    Energy Technology Data Exchange (ETDEWEB)

    Hagedorn, K.; Kraemer, S.; Lang, N. [Universitaets-Frauenklinik, Erlangen (Germany); Schulz-Wendtland, R.; Bautz, W. [Universitaet Erlangen-Nuernberg (Germany) Institut fuer Diagnostische Radiologie; Kat, S.; Wolf, F. [Universitaet Erlangen-Nuernberg (Germany). Nuklearmedizinische Klinik

    1998-03-01

    We performed {sup 99m}Tc-MIBI scintiscanning in 42 patients with indications for surgery of mammary lesions, ascertained by palpation, mammography, ultrasonography, or MRI. We had a total of 62 histologically examined tumors for our analysis, comprising 24 benign tumors (10 fibroadenomas, 1 papilloma, 1 cyst, 1 lymph node, 10 mastopathies), 4 in-situ carcinomas, and 34 malignant tumors (32 duct carcinomas, 1 intramammary lymph node matastasis, 1 low-grade malignant sarcoma). The benign mammary gland tumors were not shown by contrast agent accumulation in the {sup 99m}Tc-MIBI scintiscans. Out of 14 carcinomas of a size less than 15 mm across, only 5 were detected by scintiscanning, and none of the in-situ carcinomas was revealed in the scintiscans. However, all malignomas of a size of 15 mm or more were detected by scintiscanning. The results of the comparative analysis of the diagnostic methods relating to sensitivity and specificity are (n=58, without CIS):{sup 9}9mTc-MIBI scintiscans: 73.5%/100%; mammography: 94,1%/62.5%; palpation:82.4%/91.7%; ultrasonography:94.1%/58.3%. As compared to the other four methods, {sup 9}9mTc-MIBI scintiscanning ranks last in terms of sensitivity, and is the best method interms of specificity. The high specificity shown by our study could not be confirmed by other studies, and the sensitivity is particularly restricted when tumors are small in size, or at unfavourable locations. The conclusion therefore is that this scintiscanning method is not to be recommended as a screening method, nor is it a method of choice for complementary diagnostic evaluation of breast lesions, except perhaps for a few specific cases. (orig./CB) [Deutsch] Wir fuehrten die {sup 99m}Tc-MIBI-Szintigraphie bei 42 Patientinnen mit operationsbeduerftigem Mammabefund durch, die zuvor palpatorisch, mammographisch, sonographisch und zum Teil mittels Magnetresonanztomographie der Mamma untersucht worden waren. Insgesamt konnten wir 62 histologisch untersuchte

  14. Problems in the scintigraphic detection of osteomyelitis in children. [/sup 99m/Tc-pyrophosphate

    Energy Technology Data Exchange (ETDEWEB)

    Sullivan, D.C.; Rosenfield, N.S.; Ogden, J.; Gottschalk, A.

    1980-06-01

    /sup 99m/Tc pyrophosphate studies in 21 children with acute hematogenous osteomyelitis were compared with radiographic, clinical and surgical findings. Eleven /sup 99m/Tc studies revealed obvious abnormalities, four showed subtle abnormalities, two were misleading, and four were normal. No consistent explanation for the lack of positive radionuclide findings was found. We concluded that pediatric osteomyelitis presents a spectrum of scintigraphic appearances and interpretation is often difficult.

  15. Tc-99m Labeled HMPAO white Blood Cell Scintigraphy in Pediatric Patients

    OpenAIRE

    Funda Aydın; Arzu Kın Cengiz; Fırat Güngör

    2012-01-01

    Objective: 99mTc labeled hexamethylpropylene amine oxime (HMPAO) white blood cell (WBC) scintigraphy is a frequently used option for acute infection, particularly in pediatric patients. This scintigraphy is applied to detect sites of infection/inflammation in patients with fever of unknown origin, to find and follow up osteomyelitis, and to detect suspicion of acute appendicitis. The aim of this retrospective study was to evaluate the value of 99mTc-HMPAO labeled WBC scintigraphy in pediatric...

  16. Is Brown Adipose Tissue Visualization Reliable on 99mTc-Methoxyisobutylisonitrile Diagnostic SPECT Scintigraphy?

    Science.gov (United States)

    Haghighatafshar, Mahdi; Farhoudi, Farinaz

    2016-01-01

    The 99mTc-MIBI has been used with great value as a diagnostic technique in patients with primary hyperparathyroidism. False-positive scans may occur due to misinterpretation of the physiologic distribution of the 99mTc-MIBI. Reviewing consecutive SPECT scans, we evaluated this possibility and assessed how frequently brown adipose tissue (BAT) is seen on 99mTc-MIBI scintigraphy. Here, we retrospectively reviewed scans of consecutive patients who were evaluated for parathyroid adenomas from March 2015 to June 2015, using dual-phase (early and delayed) planar imaging and SPECT. We identified 60 patients (48 female and 12 male; mean age, 52.25 ± 15.20 years; range, 22-86 years).We detected the presence of 99mTc-MIBI uptake in BAT in 20 of 60 patients (33.33%) in the neck. Although the patients with T99mc-MIBI uptake in BAT were younger (mean age, 48.85 ± 15.27 years, range, 26-73 years) than the patients with no 99mTc-MIBI uptake (mean age, 53.95 ± 15.07 years, range, 22-86 years), this difference was not statistically significant (P = 0.224). The percentage of female patients with BAT detection was higher (17/48 patients; 37.5%) than that of the male population (3/12 patients; 25%), this difference was not also statistically significant (P = 0.85).In patient population referred to 99mTc MIBI scintigraphy of the parathyroid glands, uptake of 99mTc-MIBI in BAT should not be misinterpreted with 99mTc-MIBI-avid-tumors. Fused SPECT/CT images (not SPECT-only) are necessary to distinguish BAT from bone, muscle, thyroid, myocardium, parathyroids, and other structures in the neck and chest. PMID:26765463

  17. Mercaptoacetyltriglycine (MAG 3)-99mTc - a novel radiopharmaceutical for uropoietic system diagnosis

    International Nuclear Information System (INIS)

    Mercaptoacetyltriglycine (MAG 3) labelled with 99mTc is a novel prospective diagnostic agent for the uropoietic system which can replace injections of sodium iodohippurate labelled with 131I. Discussed are the procedure of labelling this novel radiopharmaceutical with 99mTc, evaluation of its quality by chromatographic methods, and bioavailability of MAG 3 in laboratory animals in comparison with that of injections of sodium iodohippurate labelled with 131I. (author). 8 figs., 7 refs

  18. labelling and quality control of some 99m Tc-radiopharmaceuticals of expected biological activity

    International Nuclear Information System (INIS)

    this thesis addresses the labelling and quality control of some 99mTc-radiopharmaceuticals which could be used for infection imaging. this study focuses on the labelling of sarafloxation, gatifloxation and cefepine with technetium-99m and biological evaluation of these labeled complexes and biodistribution in both normal and inflamed mice. the thesis is organized into two chapters: chapter I :labelling of some antibiotics chapter II :biological evaluation.

  19. A {sup 99m}Tc Generator using PZC for (n,{gamma}) {sup 99}Mo

    Energy Technology Data Exchange (ETDEWEB)

    Adang, H.G.; Mutalib, A.; Suparman, I.; Hamid; Purwadi, B.; Pancoko, M.; Setiowati, S.; Yulianti, V.; Robertus, D.H. [Radioisotope Production Center, National Atomic Energy Agency Kawasan PUSPIPTEK, Serpong (Indonesia)

    1998-10-01

    The high performance adsorbent Poly Zirconium Compound (PZC) was produced by Department of Radioisotope, Japan Atomic Energy Research Institute. This compound was developed as an adsorbent for natural Mo (n,{gamma}) {sup 99}Mo-{sup 99m}Tc Generator. In the present paper, we report the performance of the PZC for a {sup 99m}Tc Generator which was focused on the yield, on elution profile and {sup 99}Mo breakthrough. (author)

  20. Direct labeling of isoniazid with technetium-99m for diagnosis of tuberculosis

    International Nuclear Information System (INIS)

    Isonicotinic acid hydrazide (isoniazid) is one of the most effective agents in tuberculosis therapy. Hence it was chosen as ligand for 99mTc labeling and imaging in the developed animal model with a gamma camera. Direct labeling of isoniazid with technetium-99m was studied. Factors affecting the radiolabeling efficiency such as amount of reducing agent, pH and time of the reaction were studied. Biodistribution of the labeled compound was performed in Sprague-Dawley rats. The localization kinetics of the radiolabeled complex was also studied in the developed animal model by injecting 100-125 MBq 99mTc-isoniazid intravenously in the ear of rabbit and the images were taken with a gamma camera. Optimum conditions gave > 98% labeling efficiency of 99mTc-isoniazid. Biodistribution studies in rats revealed that the maximum uptake was in kidneys (15%, 8% and 2.5% at 0.5, 4 and 24 hours, respectively), indicating renal excretion of the 99mTc-isoniazid. High accumulation was obtained in liver (10%, 11% and 4% at 0.5, 4 and 24 hours, respectively) and significant radioactivity was also seen in the intestines (8%, 6% and 1% at 0.5, 4 and 24 hours, respectively), indicating hepatobiliary excretion of the complex. Less than 2% uptake in stomach until 24 hours confirmed good in vivo stability of the complex. 99mTc-isoniazid initially accumulated in infective lesions of S. aureus in rabbits due to hyper-vascularity, but because of its non specificity for S. aureus the residency of 99mTc-isoniazid was low and it showed rapid wash out from the lesion, whereas residency of tubercular lesion was high and it remained in the tubercular lesion in the delayed images also. The results suggest that 99mTc-isoniazid is a specific agent for localization of tubercular lesions. (orig.)

  1. Biological behavior of {sup 99m}Tc(v)DMSA in mice

    Energy Technology Data Exchange (ETDEWEB)

    Correia, Marilia B.L.; Silva, Orion M.C. da; Magnata, Simey S.S.L.P. [Universidade Federal de Pernambuco, Recife, PE (Brazil). Dept. de Energia Nuclear. Centro de Tecnologia e Geociencias]. E-mail: marilialiborio@ig.com.br; Barros, Andre L.S.; Catanho, Maria Teresa J. de A. [Universidade Federal de Pernambuco, Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia]. E-mail: mariajansem@terra.com.br; Lima, Fabiana F. de [Centro Regional de Ciencias Nucleares CRCN/NE-CNEN, Recife, PE (Brazil)]. E-mail: fflima@cnen.gov.br

    2007-07-01

    The {sup 99m}Tc(v)DMSA is a tumor-seeking agent which has been reported in bone metastasis and others tumors scintigraphies. The bone affinity and tumor uptake has been researched to elucidate its mechanism and biological behavior. The aim of this study was to obtain {sup 99m}Tc(v)DMSA from a conventional DMSA kit, to evaluate its biodistribution in an animal model, and to verify the efficiency of this method based on literature data. DMSA kit (produced by IPEN) were used to prepare {sup 99m}Tc(v)DMSA by reconstituting the lyophilized kit with 0.2mL of 3.5% NaHCO{sub 3} and addition of {sup 99m}TcO{sup 4}{sup -}. The biodistribution assays were made with mice (130, 115 and 70 days old), males and females. To each assay, the control group (n=5) received intravenous {sup 99m}Tc-DMSA and the experimental group (n=5) received {sup 99m}Tc(v)DMSA. After 30 min or 1 h, the animals were sacrificed, the organs excised and the activity measured by a gamma counter. The uptake percentage per gram (% uptake/g), tissue/blood ratio (kidney/blood - K/BL) and kidney/bone ratio (K/BO) were evaluated. In all assays there were different significant biodistribution (p{<=}0.05) between control and experimental groups, the results showed the less kidney uptake of {sup 99m}Tc(v)DMSA while increase bone affinity as young as be the animal. The {sup 99m}Tc(v)DMSA arising from DMSA kit by modified binding method was compatible with literature data. (author)

  2. Dose calibrator linearity test: 99mTc versus 18F radioisotopes*

    Science.gov (United States)

    Willegaignon, José; Sapienza, Marcelo Tatit; Coura-Filho, George Barberio; Garcez, Alexandre Teles; Alves, Carlos Eduardo Gonzalez Ribeiro; Cardona, Marissa Anabel Rivera; Gutterres, Ricardo Fraga; Buchpiguel, Carlos Alberto

    2015-01-01

    Objective The present study was aimed at evaluating the viability of replacing 18F with 99mTc in dose calibrator linearity testing. Materials and Methods The test was performed with sources of 99mTc (62 GBq) and 18F (12 GBq) whose activities were measured up to values lower than 1 MBq. Ratios and deviations between experimental and theoretical 99mTc and 18F sources activities were calculated and subsequently compared. Results Mean deviations between experimental and theoretical 99mTc and 18F sources activities were 0.56 (± 1.79)% and 0.92 (± 1.19)%, respectively. The mean ratio between activities indicated by the device for the 99mTc source as measured with the equipment pre-calibrated to measure 99mTc and 18F was 3.42 (± 0.06), and for the 18F source this ratio was 3.39 (± 0.05), values considered constant over the measurement time. Conclusion The results of the linearity test using 99mTc were compatible with those obtained with the 18F source, indicating the viability of utilizing both radioisotopes in dose calibrator linearity testing. Such information in association with the high potential of radiation exposure and costs involved in 18F acquisition suggest 99mTc as the element of choice to perform dose calibrator linearity tests in centers that use 18F, without any detriment to the procedure as well as to the quality of the nuclear medicine service. PMID:25798005

  3. Preparation of 99mTc- Tin colloid: a freeze-dried kit for liver imaging

    International Nuclear Information System (INIS)

    99mTc-tin-colloid was formulated as a useful agent for liver imaging. The radiochemical purity and stability of the labeled colloid were determined by paper chromatography and 85% methanol solvent, the labelling efficiency was > 95%. The comparative studies of the biological distribution of 99mTc-tin-colloid and 99Tc-Sulphur-colloid were made with Wistar rats. The results of organ distribution were similar in both products; > 90% liver uptake. (author)

  4. Compact device for the extraction of sup(99m)TcO4Na

    International Nuclear Information System (INIS)

    A non automatic device for the extraction of sup(99m)TcO4Na by liquid-liquid extraction method is described. It has been developed at the Laboratory of Labelled Molecules of the CNEA and was installed at the Nuclear Medicine Centre of the Hospital de Clinicas Jose de San Martin. The solutions of sup(99m)TcO4Na are used for the labelling of radiopharmaceuticals and also for making radiodiagnosis. (author)

  5. Evaluation of inflammatory bowel disease with /sup 99mTc-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Kadir, S.; Strauss, H.W.

    1979-02-01

    Technetium-99m-labelled DTPA was found to localize in segments of bowel with inflammation due to ulcerative colitis, regional enteritis, and other forms of enterocolitis. The concentration of tracer was apparently related to the clinical activity of the disease process. Imaging with /sup 99m/Tc-DTPA may offer an appealing, noninvasive alternative for identifying and following up patients with inflammatory bowel disease.

  6. Perfusion scanning using 99mTc-HMPAO detects early cerebrovascular changes in the diabetic rat

    OpenAIRE

    Fatma J Al-Saeedi

    2008-01-01

    Background 99mTc-HMPAO is a well-established isotope useful in the detection of regional cerebral blood flow. Diabetes gives rise to arterial atherosclerotic changes that can lead to significant end organ dysfunction, prominently affecting perfusion to the heart, kidneys, eyes and brain. In the current study, we investigated the role of 99mTc-HMPAO cerebral perfusion scans in detecting early vascular changes in the diabetic brain. Methods Cerebral perfusion studies were performed on both cont...

  7. Bone marrow uptake of 99mTc-MIBI in patients with multiple myeloma

    International Nuclear Information System (INIS)

    In a previous study, we showed the ability of technetium-99m methoxyisobutylisonitrile (99mTc-MIBI) scan to identify active disease in patients with multiple myeloma (Eur J Nucl Med 1998; 25: 714-720). In particular, a semiquantitative score of the extension and intensity of bone marrow uptake was derived and correlated with both the clinical status of the disease and plasma cell bone marrow infiltration. In order to estimate quantitatively 99mTc-MIBI bone marrow uptake and to verify the intracellular localization of the tracer, bone marrow samples obtained from 24 multiple myeloma patients, three patients with monoclonal gammopathy of undetermined significance (MGUS) and two healthy donors were studied for in vitro uptake. After centrifugation over Ficoll-Hypaque gradient, cell suspensions were incubated with 99mTc-MIBI and the uptake was expressed as the percentage of radioactivity specifically retained within the cells. The cellular localization of the tracer was assessed by micro-autoradiography. Twenty-two out of 27 patients underwent 99mTc-MIBI scan within a week of bone marrow sampling. Whole-body images were obtained 10 min after intravenous injection of 555 MBq of the tracer; the extension and intensity of 99mTc-MIBI uptake were graded using the semiquantitative score. A statistically significant correlation was found between in vitro uptake of 99mTc-MIBI and both plasma cell infiltration (Pearson's coefficient of correlation r=0.69, P99mTc-MIBI inside the plasma cells infiltrating the bone marrow. Therefore, our findings show that the degree of tracer uptake both in vitro and in vivo is related to the percentage of infiltrating plasma cells which accumulate the tracer in their inner compartments. (orig.)

  8. Direct labeling of isoniazid with technetium-99m for diagnosis of tuberculosis

    Energy Technology Data Exchange (ETDEWEB)

    Roohi, S.; Mushtaq, A.; Jehangir, M. [Isotope Production Div., Pakistan Inst. of Nuclear Science and Technology, P.O. Nilore, Islamabad (Pakistan); Malik, S.A. [Dept. of Biological Sciences, Quaid-e-Azam Univ., Islamabad (Pakistan)

    2006-07-01

    Isonicotinic acid hydrazide (isoniazid) is one of the most effective agents in tuberculosis therapy. Hence it was chosen as ligand for {sup 99m}Tc labeling and imaging in the developed animal model with a gamma camera. Direct labeling of isoniazid with technetium-99m was studied. Factors affecting the radiolabeling efficiency such as amount of reducing agent, pH and time of the reaction were studied. Biodistribution of the labeled compound was performed in Sprague-Dawley rats. The localization kinetics of the radiolabeled complex was also studied in the developed animal model by injecting 100-125 MBq {sup 99m}Tc-isoniazid intravenously in the ear of rabbit and the images were taken with a gamma camera. Optimum conditions gave > 98% labeling efficiency of {sup 99m}Tc-isoniazid. Biodistribution studies in rats revealed that the maximum uptake was in kidneys (15%, 8% and 2.5% at 0.5, 4 and 24 hours, respectively), indicating renal excretion of the {sup 99m}Tc-isoniazid. High accumulation was obtained in liver (10%, 11% and 4% at 0.5, 4 and 24 hours, respectively) and significant radioactivity was also seen in the intestines (8%, 6% and 1% at 0.5, 4 and 24 hours, respectively), indicating hepatobiliary excretion of the complex. Less than 2% uptake in stomach until 24 hours confirmed good in vivo stability of the complex. {sup 99m}Tc-isoniazid initially accumulated in infective lesions of S. aureus in rabbits due to hyper-vascularity, but because of its non specificity for S. aureus the residency of {sup 99m}Tc-isoniazid was low and it showed rapid wash out from the lesion, whereas residency of tubercular lesion was high and it remained in the tubercular lesion in the delayed images also. The results suggest that {sup 99m}Tc-isoniazid is a specific agent for localization of tubercular lesions. (orig.)

  9. Dose calibrator linearity test: {sup 99m}Tc versus {sup 18}F radioisotopes

    Energy Technology Data Exchange (ETDEWEB)

    Willegaignon, Jose; Coura-Filho, George Barberio; Garcez, Alexandre Teles, E-mail: willegaignon@hotmail.com [Instituto do Cancer do Estado de Sao Paulo Octavio Frias de Oliveira (ICESP), Sao Paulo, SP (Brazil); Sapienza, Marcelo Tatit; Buchpiguel, Carlos Alberto [Universidade de Sao Paulo (FM/USP), Sao Paulo, SP (Brazil). Fac. de Medicina; Alves, Carlos Eduardo Gonzalez Ribeiro; Cardona, Marissa Anabel Rivera; Gutterres, Ricardo Fraga [Comissao Nacional de Energia Nuclear (CNEN), Rio de Janeiro, RJ (Brazil)

    2015-01-15

    Objective: the present study was aimed at evaluating the viability of replacing {sup 18}F with {sup 99m}Tc in dose calibrator linearity testing. Materials and methods: the test was performed with sources of {sup 99m}Tc (62 GBq) and {sup 18}F (12 GBq) whose activities were measured up to values lower than 1 MBq. Ratios and deviations between experimental and theoretical {sup 99m}Tc and {sup 18}F sources activities were calculated and subsequently compared. Results: mean deviations between experimental and theoretical {sup 99m}Tc and {sup 18}F sources activities were 0.56 (± 1.79)% and 0.92 (± 1.19)%, respectively. The mean ratio between activities indicated by the device for the {sup 99m}Tc source as measured with the equipment precalibrated to measure {sup 99m}Tc and {sup 18}F was 3.42 (± 0.06), and for the {sup 18}F source this ratio was 3.39 (± 0.05), values considered constant over the measurement time. Conclusion: the results of the linearity test using {sup 99m}Tc were compatible with those obtained with the {sup 18}F source, indicating the viability of utilizing both radioisotopes in dose calibrator linearity testing. Such information in association with the high potential of radiation exposure and costs involved in {sup 18}F acquisition suggest {sup 99m}Tc as the element of choice to perform dose calibrator linearity tests in centers that use {sup 18}F, without any detriment to the procedure as well as to the quality of the nuclear medicine service. (author)

  10. Comparison of chromatography systems for radiochemical purity determination of lyophilized reagents labeled with technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Monteiro, Elisiane G.; Almeida, Erika V.; Ramos, Marcelo P.S.; Alves, Edson V.; Benedetti, Stella; Mengatti, Jair; Fukumori, Neuza T.O.; Matsuda, Margareth M.N., E-mail: elisianegodoy@terra.com.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2009-07-01

    A variety of lyophilized reagents (LR) labeled with {sup 99m}Tc has been developed for determining organ function or assessing disease status by imaging methods. Usually, the quality of the radiopharmaceutical preparations is evaluated by paper chromatography (PC), thin layer chromatography (TLC), instant thin layer chromatography silica gel (ITLC-SG), high performance liquid chromatography (HPLC) on reverse-phase columns and capillary electrophoresis (CE). PC and TLC have been applied due to the low cost and short time in the determination of pertechnetate ({sup 99m}TcO{sub 4}-) and technetium dioxide ({sup 99m}TcO{sub 2}). The present study reports the comparison between PC and TLC chromatographic methods for determination of the radiochemical purity of LR labeled with {sup 99m}Tc from IPEN-CNEN/SP (Brazil). PC was performed with Whatman 3MM/1MM paper chromatography strips and TLC with ITLC-SG sheets or reversed phase (RP). RP was used only for ECD. Although the radioactivity profile of the separation of the species on both stationary phases was satisfactory, the difference in results for % {sup 99m}TcO{sub 4}- and {sup 99m}TcO{sub 2} was up to 4.2 % using PC for ECD and PYP. ITLC supports gave better resolution than conventional PC supports for these products. In ECD analysis, the comparison was performed between RP and ITLC-SG stationary phases for determination of {sup 99m}TcO{sub 4}-, {sup 99m}TcO{sub 2} and other impurities. It was observed that the sheet length as described in the United States Pharmacopoeia was not sufficient for a good separation of the product and the impurities. The results showed that there were not significant differences between PC and TLC chromatographic stationary phases are going to be accomplished. (author)

  11. The retention of [99mTc]-d,l-HM-PAO in the human brain after intracarotid bolus injection

    DEFF Research Database (Denmark)

    Lassen, N A; Andersen, A R; Friberg, L;

    1988-01-01

    [99mTc]-d,l-HM-PAO (HM-PAO) was injected rapidly into the internal carotid artery and its retention in the brain was recorded by external scintillation cameras in eight human subjects. A model is described based on three compartments: the lipophilic tracer in the blood pool of the brain......, the lipophilic tracer inside the brain, and the hydrophilic form retained in the brain. The retention curve initially drops abruptly, corresponding to the nonextracted fraction of the injectate leaving the brain; it then falls exponentially towards the asymptotic level of the fractional steady-state retention R....... Cerebral blood flow (F) was measured using the xenon-133 intracarotid injection method. The first-pass extraction E of HM-PAO was calculated from F using an empiric regression equation. The residue curves for the whole brain after intracarotid HM-PAO injection were analyzed to yield a retention fraction (R...

  12. Studies of techniques for the post-elution concentration of 99mTc obtained from gel type 99Mo/99mTc generators

    International Nuclear Information System (INIS)

    On average 80% of the radiopharmaceuticals used in Nuclear Medicine are labeled with 99mTc due to its physical properties and easy attainment through of 99Mo/99mTc generators. The Directory of Radiopharmacy (DIRF) of IPEN-CNEN/SP developed a gel type chromatographic generator of MoZr with 99Mo produced by 98Mo(n,γ)99Mo reaction that occurs at the IEA-R1 Nuclear Reactor. The gel is composed of zirconium molybdate with elution volume of 12 mL with an activity of 11100 MBq (300 mCi) producing a radioactive concentration of 925 MBq (25 mCi)/mL. The fission generator gives a higher radioactive concentration around 1850 MBq (50 mCi)/mL. The aim of this work is to study a system of post-elution concentration of 99mTc for the attainment of a high enough radioactive concentration to meet the demands of the market, with a proved quality. Two types of systems of post-elution concentration were developed: the single and the tandem. The most appropriate system for the gel generator of 99Mo/99mTc, being at the same time sterile and vacuum automated, was the tandem system using Dionex 2.5 cc/QMA cartridges. The gel generator is eluted with 10 mL of solution of 0.1% NaCl and the pertechnetate anion is retained in the QMA cartridge and further eluted with 4 mL of saline. The process takes no more than 30 minutes. The elution efficiency of the system of concentration was 90 %. At the beginning of 2009 a global crisis in the supply of 99Mo took place making it necessary the development of alternative technologies for the production of 99Mo/99mTc generators using fission produced 99Mo and the development of an appropriate method to extend the useful life of this generator. The results of this study showed that the same system developed for the post- concentration of the gel generator can be employed for the fission generator, using the tandem system, giving a concentration factor of 3 for the elution of 99mTc. (author)

  13. {sup 99m}Tc-besilesomab (Scintimun {sup registered}) in peripheral osteomyelitis: comparison with {sup 99m}Tc-labelled white blood cells

    Energy Technology Data Exchange (ETDEWEB)

    Richter, Wolf S. [Pharmtrace Klinische Entwicklung GmbH, Berlin (Germany); University Clinics Magdeburg (Germany). Clinics for Radiology and Nuclear Medicine; Ivancevic, Velimir [Nuclear Medicini, Celle (Germany); Meller, Johannes [University Medicine, Goettingen (Germany). Dept. of Nuclear Medicine; Lang, Otto [UH Kralovske, Prague (Czech Republic). Dept. of Nclear Medicine; Le Guludec, Dominique [CHU Bichat-Claude Bernard, Paris (France). Service de Medecine Nucleaire; Szilvazi, Istvan [Orszagos Gyogyintezeti Koezpont, Budapest (Hungary). Dept. of Nuclear Medicine; Amthauer, Holger [University Clinics Magdeburg (Germany). Clinics for Radiology and Nuclear Medicine; Chossat, Florence; Dahmane, Amel [IBA/CIS Bio International, Gif sur Yvette (France); Schwenke, Carsten [SCOSSIS, Berlin (Germany); Signore, Alberto [University of Rome, Faculty of Medicine, Nuclear Medicine Unit, Rome (Italy)

    2011-05-15

    The diagnosis of osteomyelitis is a challenge for diagnostic imaging. Nuclear medicine procedures including white blood cell imaging have been successfully used for the identification of bone infections. This multinational, phase III clinical study in 22 European centres was undertaken to compare anti-granulocyte imaging using the murine IgG antibody besilesomab (Scintimun {sup registered}) with {sup 99m}Tc-labelled white blood cells in patients with peripheral osteomyelitis. A total of 119 patients with suspected osteomyelitis of the peripheral skeleton received {sup 99m}Tc-besilesomab and {sup 99m}Tc-hexamethylpropyleneamine oxime (HMPAO)-labelled white blood cells (WBCs) in random order 2-4 days apart. Planar images were acquired at 4 and 24 h after injection. All scintigraphic images were interpreted in an off-site blinded read by three experienced physicians specialized in nuclear medicine, followed by a fourth blinded reader for adjudication. In addition, clinical follow-up information was collected and a final diagnosis was provided by the investigators and an independent truth panel. Safety data including levels of human anti-mouse antibodies (HAMA) and vital signs were recorded. The agreement in diagnosis across all three readers between Scintimun {sup registered} and {sup 99m}Tc-HMPAO-labelled WBCs was 0.83 (lower limit of the 95% confidence interval 0.8). Using the final diagnosis of the local investigator as a reference, Scintimun {sup registered} had higher sensitivity than {sup 99m}Tc-HMPAO-labelled WBCs (74.8 vs 59.0%) at slightly lower specificity (71.8 vs 79.5%, respectively). All parameters related to patient safety (laboratory data, vital signs) did not provide evidence of an elevated risk associated with the use of Scintimun {sup registered} except for two cases of transient hypotension. HAMA were detected in 16 of 116 patients after scan (13.8%). Scintimun {sup registered} imaging is accurate, efficacious and safe in the diagnosis of peripheral

  14. 99mTc generator preparation using (n, γ)99Mo produced ex-natural molybdenum

    International Nuclear Information System (INIS)

    Theoretical assessment on the chromatographic 99mTc generator preparation using (n, γ) 99Mo produced ex-natural molybdenum was carried out. The relationship between the neutron flux for MoO3 target activation, Mo-content or Mo adsorption capacity of column packing material, 99mTc pertechnetate concentration and/or 99mTc radioactivity of eluate was established. The reasonably lower limit of neutron flux of reactor and Molybdenum content of column packing material were found out to estimate the production of portable chromatographic generators available for nuclear medicine application. The concentration of 99mTc pertechnetate eluate of low 99mTc concentration using the column elution technique was also evaluate theoretically and conducted successfully in practice. Three options of 99mTc generator using Titanium-Molybdate, Zirconium-Molybdate and Zirconium Oxide as generator column-packing materials were prepared and successfully put into use in nuclear medicine application. (author)

  15. Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m

    Institute of Scientific and Technical Information of China (English)

    ABREU P.R.C.; ALMEIDA M.C.; BERNARDO R.M.; BERNARDO L.C.; BRITO L.C.; GARCIA E.A.C.; FONSECA A.S.; BERNARDO-FILHO M.

    2006-01-01

    Psidium guajava (guava) leaf is a phytotherapic used in folk medicine to treat gastrointestinal and respiratory disturbances and is used as anti-inflammatory medicine. In nuclear medicine, blood constituents (BC) are labelled with technetium-99m (99mTc) and used to image procedures. However, data have demonstrated that synthetic or natural drugs could modify the labelling of BC with 99mTc. The aim of this work was to evaluate the effects of aqueous extract of guava leaves on the labelling of BC with 99mTc. Blood samples of Wistar rats were incubated with different concentrations of guava extract and labelled with 99mTc after the percentage of incorporated radioactivity (%ATI) in BC was determined. The results suggest that aqueous guava extract could present antioxidant action and/or alters the membrane structures involved in ion transport into cells, thus decreasing the radiolabelling of BC with 99mTc. The data showed significant (P<0.05) alteration of ATI in BC from blood incubated with guava extract.

  16. Acetylsalicylic acid and labeling of blood constituents with technetium-99m

    Energy Technology Data Exchange (ETDEWEB)

    Fonseca, Adenilson de Souza da [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Dept. de Farmacologia e Psicobiologia; Frydman, Jacques Natan Grinapel; Rocha, Vanessa Camara da; Bernardo-Filho, Mario [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Inst. de Biologia Roberto Alcantara Gomes. Dept. de Biofisica e Biometria

    2005-10-15

    Acetylsalicylic acid is the drug most used an anti-inflammatory agent and for secondary prevention of thrombotic phenomenon. Drugs can modify the labeling of blood constituents with technetium-99m (99m Tc). The aim of this work was to evaluate the effect of in vitro or in vivo assays with acetylsalicylic acid on the labeling of the blood constituents with 99m Tc. In vitro assay was performed with samples of whole blood from Wistar rats incubated with acetylsalicylic acid (1.0 mg/ml) for one hour before the 99m Tc-labeling process. For in vivo assay, Wistar rats were treated with acetylsalicylic acid (1.5 mg/kg) during one hour, and the whole blood was withdrawn for the 99m Tc-labeling process. Saline was used in control groups. Data showed that the fixation of 99m Tc to the blood constituents was not significantly (p>0.05) modified in in vitro and in vivo assays with acetylsalicylic acid, at least not when the experiments were carried out with the doses normally used in human beings. (author)

  17. Comparison between high-resolution computed tomography and 99mTc-technegas SPECT pulmonary emphysema

    International Nuclear Information System (INIS)

    Scintigraphy with 99mTc-technegas was recently introduced for clinical imaging of lung ventilation. This method has been found to be useful in emergencies, to be more suitable for single photon emission computed tomography (SPECT) than other agents used in ventilation scintigraphy, and could reveal abnormalities in ventilation more easily than high resolution computed tomography (HRCT) in pulmonary emphysema. We compared 99mTc-technegas SPECT with HRCT in six regions: the right upper, middle, and lower lobes, the left upper lobe, the lingula, and the left lower lobe, in 15 patients with pulmonary emphysema. Patients with centrilobular emphysema tended to show stronger changes in upper lobes than in lower lobes on both 99mTc-technegas SPECT and HRCT. Some regions showed no change on HRCT but various changes on 99mTc-SPECT. Patients with panlobular emphysema showed severe changes on 99mTc-SPECT in lower lung fields in which well-demarcated areas of low attenuation were not seen on HRCT. We conclude that 99mTc-SPECT is useful for detecting early changes and panlobular changes in pulmonary emphysema. (author)

  18. Development of a novel 99mTc-labelled brain perfusion agent

    International Nuclear Information System (INIS)

    A method has been described for radiolabeling of salbutamol with technetium-99m (99mTc). To a 1 ml solution, containing 5 mg salbutamol and 2 mg of ascorbic acid, was added a clear solution (10 μl) of SnCl2.2H2O (100 μg) in distilled water. The pH of the solution was adjusted to 8.5, mixed gently with 10 mCi (370 MBq) of 99mTc elute and incubated at room temperature for 15 minutes. The resulting solution was passed through 0.22 μ filter and radiolabeled fraction was quantified using paper chromatography. The radiochemical analysis, employing the use of above mentioned radioanalytical technique, revealed that greater than 97% of the radioactivity was bound to salbutamol and rest of the activity was in the form of free pertechnitate (99mTcO4). Biological data, obtained after i. v. injection of 99mTc-Salbutamol to female albino rabbits, revealed a fair uptake in the brain at 30 min and 60 min post injection time intervals. On the basis of animal biodistribution data it is suggested that 99mTc-salbutamol can be successfully applied as a brain perfusion agent. (author)

  19. Tc-99m-EDDA-HYNIC-TOC somatostatin receptor scintigraphy in patients with carcinoids

    Directory of Open Access Journals (Sweden)

    Vlajković Marina

    2014-11-01

    Full Text Available The aim of this paper is to determine the influence of Ki-67 proliferation index on somatostatin receptor scintigraphy (SSRS with Tc-99m-EDDA-HYNIC-TOC (Tc-99m-Tektrotyd somatostatin analogue in patients with carcinoid tumors. Sixty-one patients (31 female, 30 male; age range: 33-76 years were examined: 13 patients highly suspected of having a carcinoid, and 48 patients who had undergone the surgical removal of the tumor. Whole body SSRS at 4 h postinjection, spot scintigrams and SPECT of the selected regions were obtained for all patients. Tc-99m-Tektrotyd scintigraphy was classified as true positive in 26 out of 30 and true negative in 24 out of 28 patients. The sensitivity of Tc-99mTc-Tektrotyd scintigraphy was found to be as high as 94.74% in the group of patients with low mitotic index Ki67 (20%. The likelihood of Tc-99m-Tektrotyd scan being positive when a carcinoid is present was found to be inversely proportional to the value of Ki67 proliferation index. The results showed that Tc-99m-Tektrotyd SSRS is a sensitive method for diagnosing and staging patients with well-differentiated carcinoid tumors. However, in poorly differentiated tumors with high Ki67 proliferation index, additional analyses are necessary for precise staging.

  20. Radiochemical quality control of kits labelled with Tc-99m produced at IPEN-CNEN/SP

    International Nuclear Information System (INIS)

    The radiopharmaceuticals labelled with Tc-99m are routinely used in Nuclear Medicine Laboratories. A large number of these employ tin (II) reagents to reduce Tc (pertechnetate-VII) to a lower valence state thereby making it more able to complex forming reactions. The miniaturized chromatography system of Tc-99m labelled compounds using Whatman 3MM (8 x 1 cm) as a support and 30% NaC1: 0,9% 'NaC1: 85% MeOH and acetone as a solvent permits to assay the radiochemical purity in a few minutes after preparation. In addition this method introduced in routine work not only determines Tc-99m (pertechnetate) but also determines reduced Tc - 99m unbound to the radiopharmaceuticals (hydrolyzed reduced Tc-99m). The lyophilized kits for labelling with Tc-99m produced at IPEN-CNEN/SP are: MDP, DTPA, HSA, GHA, HIDA, Pyro, MAA, MIAA, Sulfur Colloid, Dextran-500, Sn.Cit. and Phytate. Radiochemical quality control of these kits were performed at the first day of preparation and during 12 months for determining' their validity for use. All preparation showed high yield of labelling (95-99%) during this period of time. (author)

  1. Preparation of the 99mTc-dextran lymphoscin tigraphic agent and its preliminary clinical application

    International Nuclear Information System (INIS)

    Dextran-70 was treated by gradient sedimentation, and that with a mean molecular weight of 105000 which was optimal for lymphatic system imaging rabbits was selected. Sn-Dextran kit was produced containing the above-mentioned dextran. Sn-Dextran kits can be stored at 4 deg C for 8 months. Its efficiency of labelling is more than 95%. The biodistribution, pharmacokinetics studies and lymphoscintigraphy in rabbits for 99mTc-dextran showed: (1) high radioactivity in the popliteal nodes and very low redioactivity in other nontarget organs except kidneys through which it was excreted; (2) 99mTc-dextran accumulated markedly and rapidly in lymphatic system. The lymph channels and nodes were well visualized in scintigrams; (3) 99mTc-dextran cleared rapidly from the injection site. Radioactivity of 95% of the injected dose disappearing after 6.5h. Toxicity tests indicated that: 99mTc-dextran is a drug of low toxicity and safe. Preliminary clinical applications of 99mTc-dextran in 100 cases provided satisfactory results. The process of imaging usually took less than 1h after injection, and no side-effects occurred in any patient. In most cases, the results corresponded basically with the clinical diagnosis. Hence, 99mTc-dextran is an ideal radiopharmaceutical that can be used for the visualization of the lymphatic system. It is recomminded for routine use in clincal practice

  2. 99m Tc-Cistine-Glucosamine in the evaluation of oncologic patients

    International Nuclear Information System (INIS)

    Full text: 18F fluorodeoxyglucose (FDG) PET imaging has been used to image different tumors. Due to the high cost and limited availability of PET scanners, this procedure is not accessible to many patients. Therefore, as an alternative to the expensive 18F-FDG, there is a need to develop 99mTc based agents suitable for routine clinical use with SPECT. Glucosamine(G) is a six-carbon amino sugar, that enters the cells by way of a glucose transporter systems and then by way of phosphate and glycolysis pathway forms glucosamine-6-phosphate. G labeled with 18F have shown increased uptake in tumors. By reacting G with ethylenedicysteine (EC) and using carbodiimide as coupling agent, it has been labelled with 99mTc (99mTc-ECDG). In animals, it has shown increased cellular uptake in breast and lung tumor. Its use as a tumor imaging has been tested in patients with different type of cancer. In the present study we used cistine (99mTcCG), in place of EC. The aim of this study was to evaluate the diagnostic value of 99mTc-CG as an imaging agent for tumors. The 99mTc-CG was prepared by diluting 5mg of cistine with 1ml of Sodium Hydroxide (0.1N) and conjugating it to 10mg glucosamine using 1mg N-hydroxysulfosuccinimide and 1mg carbodiimide as coupling agents. The pH was adjusted to 7.0 with NaOH and the mixtures were incubated at room temperature for 24 hrs. The compound was labeled with 99mTc in the presence of stannous chloride (1mg) and incubated at room temperature for 30 min. Sixteen patients (8 F, 8 M, mean age 60, range age 40- 79 years) with suspected primary, recurrent, or metastatic tumors based on clinical, mammography, CT/MRI, or histopathology finding were subjected to 99mTc-CG scintigraphy. Of these 16 patients 4 were of breast tumor, 1 head and neck cancer, 3 colon cancer, (2 of them with lung metastases and the other with pelvis recurrence), 5 lung cancer, and 1 patient of esophageal cancer. Two patients showed suspected breast lesions on mammography. Patients

  3. Fraction of Tc99m-incorporations in the workplace activity in nuclear medicine; Anteil von Inkorporationen an der Arbeitsplatzaktivitaet fuer TC-99M in nuklearmedizinischen Einrichtungen

    Energy Technology Data Exchange (ETDEWEB)

    Schoenmuth, T. [VKTA Rossendorf (Germany); Alborzi, H. [LfULG Sachsen (Germany); Andeeff, M. [Uniklinikum Dresden (Germany); Lorenz, J. [SMUL Sachsen (Germany); Petzold, J. [Uniklinikum Leipzig (Germany)

    2009-07-01

    In the nuclear medicine, in particular in the area of the diagnostics, Tc-99m-Pharmaka are most often used. Besides, no regular monitoring occurred in praxi up to now. With use of the factors recommended, the requirement threshold (1 mSv committed dose in the calendar year) can be crossed in many areas. Within the scope of a study employees of different nuclear-medical facilities were examined in the period from March to July 2008. In the result of more than 200 measurements was founded out that a regular incorporation monitoring is not necessary for the examined employees. The estimated dose values (actual committed dose in the calendar year) was by the majority in an area below 0.1 mSv. If single evaluations of dose values are necessary an incorporation factor of 1E-07 is recommended by using connections on the basis of Tc-99m in the area of the nuclear-medical. (orig.)

  4. Contact thermography, 99mTc-plasmin scintimetry and 99mTc-plasmin scintigraphy as screening methods for deep venous thrombosis following major hip surgery

    DEFF Research Database (Denmark)

    Christensen, S W; Wille-Jørgensen, P; Kjaer, L;

    1987-01-01

    Fifty-six patients scheduled for total hip alloplasty were screened for deep venous thrombosis by means of 99mTc-plasmin scintimetry, 99mTc-plasmin scintigraphy and contact thermography. Investigations were performed on the seventh postoperative day, and a total of 112 legs were examined. Bilateral...... ascending phlebography was used as reference procedure, and the criteria for deep venous thrombosis were intraluminal filling defects at phlebography. Six patients developed unilateral deep venous thrombosis. All three screening procedures revealed many false positive and several false negative results....... The nosographic sensitivity/specificity was 33%/75% for scintimetry, 50%/91% for scintigraphy and 33%/87% for contact thermography, respectively. It is concluded that all three tests are of no value as screening methods for deep venous thrombosis following major elective hip surgery....

  5. Development of 99mTc labelled somatostatin analogues with high affinity for somatostatin receptors

    International Nuclear Information System (INIS)

    A recent development in oncology involves the use of metabolically stabilized peptide hormone analogues labelled with metallic radionuclides for the diagnosis or therapy of malignant disease. This approach was successfully applied for the first time in the visualization of somatostatin positive tumours and their metastases with 111In DTPA-octreotide. In an effort to obtain a 99mTc somatostatin receptor affine radioligand we describe herein the synthesis, radiochemistry and preliminary biological evaluation of two novel 99mTc labelled somatostatin analogues, N4-TOC and N4-RC-160. In these compounds a tetraamine bifunctional unit was covalently attached to the N-terminal (D)Phe1 of the peptide chain using Boc-protection strategies. The peptide conjugates were purified by high performance liquid chromatography (HPLC) and characterized by UV/Vis and ES-MS spectroscopies. As revealed by HPLC, 99mTc labelling was quantitative under mild conditions, leading to a single 99mTc species in high specific activities. Affinity of 99mTc N4-TOC for the somatostatin receptor, as determined by in vitro binding assays in rat brain cortex membranes, was found unaffected by the presence of the bulky metal chelate. The binding properties of 99mTc N4-RC-160 could not be determined by this assay due to an extremely high non-specific binding of this radioligand, and will be shortly investigated by other methods. Tissue distribution in healthy mice revealed that 99mTc N4-TOC is clearing mainly through the kidneys and the urinary tract whereas 99mTc N4-RC-160 shows a high accumulation in the liver as a result of its lipophilicity. Analysis of urine samples by HPLC showed that 99mTc N4-TOC is excreted integer from the body of mice, while 99mTc N4-RC-160 is totally transformed to an unidentified hydrophilic metabolite in vivo. The location of this metabolism is currently investigated. In vivo blocking experiments using animals pre-treated with 50 μg octreotide prior to the injection of the

  6. 99mTc somatostatin imaging for detection of somatostatin receptor. A preliminary result

    International Nuclear Information System (INIS)

    Radiolabeled somatostatin analogs are used with great success in vivo for scintigraphy of somatostatin receptor and are of interest for clinical routine application as 111In octreotide is not optimal with respect to radionuclide properties, availability and cost. Objective: To validate the utility of 99mTc-somatostatin receptor (99mTcSR) imaging in clinical practice Methods: Twelve patients (5 M, 7 F, age range 2-75 yr) were studied. Three normal subject were studied for biodistribution of 99mTcSR. Nine patients had histological diagnostic of tumors (lung cancer 3), neuroblastoma, (2), pheochromocytoma (1), lymphoma (2), (1 with Hodgkin's disease (HD) and 1 with non Hodgkin's lymphoma (NHL)), meningioma (1). The 99mTC-SR was labeling following the method [99mTc (H2O)3(CO)3] + (J.Nucl Med 1999;40:1913-1917), and adding the commercially available octreotide (sandostatin) with DTPA as a chelator and tricine as a coligand. Patients were injected with 20-25 99mTc SR, and the scintigraphies were obtaining 1-5-24 hrs according to routine whole body scanning. The 99mTc SR was cleared from the circulation by the kidney and liver. Liver activity is excreted in the biliary system. The images at 4 hours show low activity in the G.I. tract. Scintigraphic images were evaluated and scored according to a) image acceptability: (1:excellent, 2:good, 3:fair, 4:poor), b) lesion uptake: (1:minimal,2:moderate,3:high), by 2 nuclear medicine physicians independently. Criteria for 99mTc RS positive finding were a focally increased uptake that persistent or increased over time, in these cases SPECT was always performed at 5 hours. Results: According to the scintigraphics finding the patients with tumors were divided in: 1) negative studies: 4 patients showed not abnormal uptake, and 2) positive studies: five patients (55,5%) showed moderate or high uptake, at 1-5-24 hours planar imaging. In these patients SPECT images at 5 hours identify the lesions with more certainty. According to

  7. Breast dosimetry in complementary radiotherapy with sodium pertechnetate-{sup 99m}-Tc (Na{sup 99m}TcO{sub 4}{sup -}) balloon at neoplasia

    Energy Technology Data Exchange (ETDEWEB)

    Lima, Carla Flavia de; Campos, Tarcisio P. R. de, E-mail: tprcampos@pq.cnpq.br, E-mail: carla.flavia@terra.com.br [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, BH (Brazil). Departamento de Engenharia Nuclear. Programa de Ciencias e Tecnicas Nucleares

    2013-07-01

    A dosimetric analyzes was performed at breast tissue in which a sodium pertechnetate-{sup 99m}Tc filled balloon was placed. An additional dose booster can be used as a complementary method to the surgical treatment in patients with breast cancer. The methodology involved the development of dosimetry in a physical phantom and a computational voxel model. Radiochromic films were calibrated providing dose versus optical density (OD) response from sample films taken at the surface of the balloon up to 10 cm far with the theoretical dose provided by MCNP modeling in water-equivalent model. A voxel model of the breast, developed at the SISCODES software, with an filled balloon inside was simulated at the MCNP code in order to generate the spatial dose distribution. Spatial dose distributions and the doses at surfaces of the breast, including those received in the chest wall, skin and lung were generated. The dosimetric results allow validating the dose in the tumor bed and adjacent health tissues. The simulations show that the application of sodium pertechnetate-{sup 99m}Tc implies high dose in the breast tissue adjacent to the tumor and preserves vital adjacent structures. As conclusion, the balloon presents itself as a viable option for the adjunctive treatment of breast cancer in patients who have appropriate indication. Irradiation with sodium pertechnetate-{sup 99m}Tc generates high doses in breast tissue and consequently in the tumor bed. Adjuvant radiation therapy in situ with sodium pertechnetate-{sup 99m}Tc balloon has low cost, availability and reduced time of treatment, decreasing the side effects of conventional radiotherapy. In progress, the dose versus OD mathematical representation will be used to identify absorbed doses at planar film placed at a breast phantom in order to generate maps of breast doses. (author)

  8. Part of the HIG-99mTc in the inflammatory illness of intestine. Role de la HIG-99mTc dans la maladie inflammatoire intestinale

    Energy Technology Data Exchange (ETDEWEB)

    Lancha, C.; Curto, L.M.M.; Mitjavila, M.; Vila, T. (Hopital Emaon et Cajal, Madrid (Spain)); Prat, E.; Abos, M.D.; Banzo, J.; Delgado, M. (Hopital Universitaire, Zaragaza (Spain))

    1993-04-01

    The diagnosis of intestine inflammatory illness is based on endoscopy, radiological exploration with contrast product and histological study. This kind of exploration can be contra-indicated during acute episode because of serious complications. Several methods with isotopes were applied because of their low aggressiveness. We realized a study in the aim to precise the role of (HIG)-99mTc (non specific human immunoglobulin) in the evaluation of diagnosis of the intestine inflammatory illness.

  9. Minimizing {sup 99m}Tc incorporation of the staff during lung ventilation studies; Verringerung der Inkorporation von {sup 99m}Technetium beim Personal waehrend Lungenventilationsuntersuchungen

    Energy Technology Data Exchange (ETDEWEB)

    Petzold, J.; Fundke, R.; Petzold, L.; Sabri, O.; Seese, A. [Universitaetsklinikum Leipzig (Germany). Klinik und Poliklinik fuer Nuklearmedizin; Alborzi, H. [Universitaetsklinikum Dresden (Germany)

    2007-07-01

    The causes of incorporation of {sup 99m}Tc of the staff during nuclear medical lung ventilation studies are investigated with the aim of minimizing it. It is shown that the incorporation of medical staff can be considerably reduced from more than 500 kBq per lung study to less than 500 Bq by some simple modifications of the technical equipment and small changes in the examination procedure. (orig.)

  10. Efficacy of 99mTc-MDP in treatment of osteoporosis%99mTc-MDP治疗骨质疏松症疗效评价

    Institute of Scientific and Technical Information of China (English)

    郭忠; 张彩杰; 吴乃宝; 张维奇

    2009-01-01

    目的 评价稳定性核素99m锝-亚甲基二膦酸盐(99mTc-MDP)治疗骨质疏松症的效果.方法 93例骨质疏松症患者随机分为治疗组58例与对照组35例.治疗组采用99mTc-MDP注射治疗:99mTc 10 ml及MDP 10 ml加入0.9%氯化钠注射液50 ml中静脉滴注,1次/d,10~15 d为1个疗程;对照组常规口服钙剂及维生素D制剂.分别观察2组治疗前及治疗3、6、12个月时血中骨钙素(BGP)、骨特异性碱性磷酸酶(BALP)及骨密度(BMD)的变化.结果 治疗组治疗3个月后BMD、BGP值出现增高,在6个月时明显增高(P均<0.05);BALP值逐渐降低,与治疗前比较差异有统计学意义(P均<0.05);对照组治疗前后3项指标均无变化.治疗6、12个月时2组各指标的比较差异有统计学意义(P均<0.05).结论 99mTc-MDP治疗能较快地增加机体BMD,降低骨吸收,增加骨强度,对骨质疏松症有较好疗效.

  11. Dimer of the peptide cycle (Ar-Gly-Asp-D-Phe-Lys) radiolabeled with 99mTc for the integrin s over-expression image: formulation, biokinetics and dosimetry

    International Nuclear Information System (INIS)

    In breast cancer, α(v)β(3) and/or α(v)β(5) integrin s are over-expressed in both endothelial and tumour cells. Radiolabeled peptides based on the RGD (Arg-Gly-Asp) sequence are radiopharmaceuticals with high affinity and selectivity for those integrin s. The RGD-dimer peptide (E-[c(RGDfK)]2) radiolabeled with 99mTc has been reported as a radiopharmaceutical with 10-fold higher affinity for the α(v)β(3) integrin as compared to the RGD-monomer. EDDA (Ethylenediamine-N,N-diacetic acid) is a hydrophilic molecule that may favours renal excretion when used as coligand in the 99mTc labelling of HYNIC-peptides and can easily be formulated in a lyophilized kit. Aim: Establish a biokinetic model for 99mTc-EDDA/HYNIC-E-[c(RGDfK)]2 prepared from lyophilized kits and evaluate the dosimetry as breast cancer imaging agent. Methods: 99mTc labelling was performed by addition of sodium pertechnetate solution and 0.2 M phosphate buffer ph 7.0 to a lyophilized formulation containing E-[c(RGDfK)]2, EDDA, tricine, mannitol and stannous chloride. Radiochemical purity was evaluated by reversed phase HPLC and ITLC-SG analyses. Stability studies in human serum were carried out by size-exclusion HPLC. In-vitro cell uptake was tested using breast cancer cells (MCF7, T47D and MDA-MB-231) with blocked and non-blocked receptors. Biodistribution and tumour uptake were determined in MCF7 tumour-bearing nude mice with blocked and non-blocked receptors, and images were obtained using a micro-SPECT/CT. Whole-body images from seven healthy women were acquired at 1, 3, 6 and 24 h after 99mTc-EDDA/HYNIC-E-[c(RGDfK)]2 administration obtained with radiochemical purities of >94 %. Regions of interest (ROIs) were drawn around source organs on each time frame. Each ROI was converted to activity using the conjugate view counting method. The image sequence was used to extrapolate 99mTc-EDDA/HYNIC-E-[c(RGDfK)]2 time-activity curves in each organ in order to adjust the biokinetic model and to calculate the