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Sample records for 7-days moxifloxacin based

  1. Antimicrobial resistance of H. pylori to the outcome of 10-days vs. 7-days Moxifloxacin based therapy for the eradication: a randomized controlled trial

    OpenAIRE

    Tomić Monika; Bakula Vinko; Kućišec Nastja; Belošić-Halle Željka; Majstorović Karolina; Bago Josip; Bago Petra; Troskot Rosana

    2010-01-01

    Abstract Introduction Antibiotic resistance decreases success of Helicobacter pylori (Hp) eradication. Recently published results show low rate of resistance and better compliance with moxifloxacin based regiments. Aims&methods Whether 7 days moxifloxacin with lansoprasole and amoxycillin can be compared with 10 days moxifloxacin with lansoprasole and amoxycillin according to moxifloxacin resistance. Patients with non-ulcer dyspepsia who had culture and histology positive Hp infection (n = 15...

  2. Moxifloxacin

    Science.gov (United States)

    ... is used to treat certain infections such as pneumonia, bronchitis, and sinus, skin, and abdominal (stomach area) ... skin or eyes dark urine decreased urination seizures unusual bruising or bleeding joint or muscle pain Moxifloxacin ...

  3. Drug-drug interactions between moxifloxacin and rifampicin based on pharmacokinetics in vivo in rats.

    Science.gov (United States)

    Huang, Lifei; Liu, Jiajun; Yu, Xin; Shi, Lei; Liu, Jian; Xiao, Heping; Huang, Yi

    2016-10-01

    Moxifloxacin and rifampicin are all the first-line options for the treatment of active tuberculosis, which are often combined for the treatment of multidrug resistance pulmonary tuberculosis in clinic. However, the potential drug-drug interactions between moxifloxacin and rifampicin were unknown. The aim of this study was to investigate the drug-drug interactions between moxifloxacin and rifampicin based on their pharmacokinetics in vivo after oral administration of the single drug and both drugs, and reveal their mutual effects on their pharmacokinetics. Eighteen male Sprague-Dawley rats were randomly assigned to three groups: moxifloxacin group, rifampicin group and moxifloxacin + rifampicin group. Plasma concentrations of moxifloxacin and rifampicin were determined using LC-MS at the designated time points after drug administration, and the main pharmacokinetic parameters were calculated. In addition, effects of moxifloxacin and rifampicin on their metabolic rate and absorption were investigated using rat liver microsome incubation systems and Caco-2 cell transwell model. The main pharmacokinetic parameters of moxifloxacin including Tmax , Cmax , t1/2 and AUC(0-t) increased more in the moxifloxacin + rifampicin group than in the moxifloxacin group, but the difference was not significant (p > 0.05). However, the pharmacokinetic parameters of rifampicin, including peak concentration, area under the concentration-time curve, half-life and the area under the first moment plasma concentration-time curve, increased significantly (p 0.05). The rat liver microsome incubation experiment indicated that moxifloxacin could increase the metabolic rate of rifampicin from 23.7 to 38.7 min. However, the Caco-2 cell transwell experiment showed that moxifloxacin could not affect the absorption rate of rifampicin. These changes could enhance the drug efficacy, but they could also cause drug accumulation, which might induce adverse effect, so it was suggested that the drug dosage

  4. Isoniazid or Moxifloxacin in Rifapentine-based Regimens for Experimental Tuberculosis?

    OpenAIRE

    Rosenthal, Ian M; Zhang, Ming; Almeida, Deepak; Jacques H Grosset; Eric L Nuermberger

    2008-01-01

    Rationale: Recent studies have demonstrated that combined substitutions of rifapentine for rifampin and moxifloxacin for isoniazid in the standard, daily, short-course regimen of rifampin, isoniazid, and pyrazinamide produces stable cure in 12 weeks or less. This study was designed to more precisely evaluate the contribution of moxifloxacin and isoniazid to rifapentine-based regimens.

  5. Moxifloxacin Population Pharmacokinetics and Model-Based Comparison of Efficacy between Moxifloxacin and Ofloxacin in African Patients

    OpenAIRE

    Zvada, Simbarashe P.; Denti, Paolo; Sirgel, Frederick A.; Chigutsa, Emmanuel; Hatherill, Mark; Charalambous, Salome; Mungofa, Stanley; Wiesner, Lubbe; Simonsson, Ulrika S. H.; Jindani, Amina; Harrison, Thomas; McIlleron, Helen M.

    2014-01-01

    Pharmacokinetic exposure and the MIC of fluoroquinolones are important determinants of their efficacy against Mycobacterium tuberculosis. Population modeling was used to describe the steady-state plasma pharmacokinetics of moxifloxacin in 241 tuberculosis (TB) patients in southern Africa. Monte Carlo simulations were applied to obtain the area under the unbound concentration-time curve from 0 to 24 h (fAUC0–24) after daily doses of 400 mg or 800 mg moxifloxacin and 800 mg ofloxacin. The MIC d...

  6. Moxifloxacin Ophthalmic

    Science.gov (United States)

    ... sore throat, fever, chills and other signs of infection ear pain or fullness rash hives itching difficulty breathing or swallowing swelling of the face, throat, tongue, lips, eyes, hands, feet, ankles, or lower legs Moxifloxacin eye drops may cause other side ...

  7. The efficacy of moxifloxacin-based triple therapy in treatment of Helicobacter pylori infection: a systematic review and meta-analysis of randomized clinical trials

    Directory of Open Access Journals (Sweden)

    G. Zhang

    2013-08-01

    Full Text Available Recent evidence shows that moxifloxacin could exert an antimicrobial effect against Helicobacter pylori in both in vitro and in vivo models. To systematically evaluate whether moxifloxacin-containing triple therapy could improve eradication rates and reduce side effects in first-line or second-line anti-H. pylori treatment, eligible articles were identified by searches of electronic databases. We included all randomized trials comparing moxifloxacin-based triple therapy with standard triple or quadruple therapy during H. pylori eradication treatment. Statistical analysis was performed with Review Manager 5.0.10. Subanalysis/sensitivity analysis was also performed. We identified seven randomized trials (n=1263. Pooled H. pylori eradication rates were 79.03% (95%CI: 75.73-82.07 and 68.33% (95%CI: 64.44-72.04 for patients with moxifloxacin-based triple therapy or with standard triple or quadruple therapy, respectively (intention-to-treat analysis. The odds ratio (OR was 1.82 (95%CI: 1.17-2.81, the occurrence of total side effects was 15.23% (95%CI: 12.58-18.20 and 27.17% (95%CI: 23.64-30.92 for groups with or without moxifloxacin, and the summary OR was 0.45 (95%CI: 0.26-0.77. In subgroup analyses, we noted that the second-line eradication rate in the moxifloxacin group was significantly higher than that in the quadruple therapy group (73.33 vs 60.17%, OR: 1.78, 95%CI: 1.16-2.73, P<0.001. However, there was no difference in first-line eradication treatment. Findings from this meta-analysis suggest that moxifloxacin-based triple therapy is more effective and better tolerated than standard triple or quadruple therapy. Therefore, a moxifloxacin-based triple regimen should be used in the second-line treatment of H. pylori infection.

  8. Repeated Administration of High-Dose Intermittent Rifapentine Reduces Rifapentine and Moxifloxacin Plasma Concentrations▿

    OpenAIRE

    Dooley, Kelly; Flexner, Charles; Hackman, Judith; Peloquin, Charles A.; Nuermberger, Eric; Chaisson, Richard E.; Dorman, Susan E.

    2008-01-01

    Moxifloxacin- and rifapentine-based regimens are under investigation for the treatment of tuberculosis. However, rifapentine may induce enzymes that metabolize moxifloxacin, resulting in decreased moxifloxacin concentrations. In this phase I, two-period, sequential-design study, 13 subjects received 400 mg moxifloxacin daily for 4 days followed by daily moxifloxacin coadministered with 900 mg rifapentine thrice weekly. Pharmacokinetic analyses were performed after the 4th and 19th doses of mo...

  9. Moxifloxacin-warfarin interaction

    OpenAIRE

    Ji, Yan; Hokayem, Youssef

    2012-01-01

    Two case reports presented here show elevated prothrombin time/international normalized ratios (PT/INR) following coadministration of warfarin and moxifloxacin. Although the underlying mechanism of this interaction still remains unclear, health care providers should be careful when prescribing moxifloxacin to patients on warfarin therapy, especially to patients with low albumin levels. More frequent monitoring of INR in these patients may be warranted.Keywords: warfarin; moxifloxacin; PT/INR;...

  10. Increased Moxifloxacin Utilization Associated with an Unrestricted Addition to a Drug Reimbursement Formulary: A Population-Based Analysis

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    Alissa Jade Wright

    2014-01-01

    Full Text Available OBJECTIVES:To determine whether utilization of moxifloxacin, a broad-spectrum fluoroquinolone antibiotic, has changed since its addition to the British Columbia provincial formulary in 2009 and to determine whether utilization was guideline concordant.

  11. In Vivo Efficacy of Moxifloxacin Compared with Cloxacillin and Vancomycin in a Staphylococcus aureus Rabbit Arthritis Experimental Model▿

    OpenAIRE

    Grossi, Olivier; Caillon, Jocelyne; Arvieux, Cedric; Jacqueline, Cedric; Bugnon, Denis; Potel, Gilles; Hamel, Antoine

    2007-01-01

    We investigated the efficacies of moxifloxacin, cloxacillin, and vancomycin in a rabbit model of Staphylococcus aureus arthritis. No significant difference between therapeutic regimens was observed after a 7-day treatment. Oral moxifloxacin could be a suitable alternative to standard parenteral therapy for S. aureus arthritis.

  12. Moxifloxacin-warfarin interaction

    Directory of Open Access Journals (Sweden)

    Yan Ji

    2012-01-01

    Full Text Available Two case reports presented here show elevated prothrombin time/international normalized ratios (PT/INR following coadministration of warfarin and moxifloxacin. Although the underlying mechanism of this interaction still remains unclear, health care providers should be careful when prescribing moxifloxacin to patients on warfarin therapy, especially to patients with low albumin levels. More frequent monitoring of INR in these patients may be warranted.

  13. A Phase 2 Randomized Trial of a Rifapentine plus Moxifloxacin-Based Regimen for Treatment of Pulmonary Tuberculosis.

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    Marcus B Conde

    Full Text Available The combination of rifapentine and moxifloxacin administered daily with other anti-tuberculosis drugs is highly active in mouse models of tuberculosis chemotherapy. The objective of this phase 2 clinical trial was to determine the bactericidal activity, safety, and tolerability of a regimen comprised of rifapentine, moxifloxacin, isoniazid, and pyrazinamide administered daily during the first 8 weeks of pulmonary tuberculosis treatment.Adults with sputum smear-positive pulmonary tuberculosis were randomized to receive either rifapentine (approximately 7.5 mg/kg plus moxifloxacin (investigational arm, or rifampin (approximately 10 mg/kg plus ethambutol (control daily for 8 weeks, along with isoniazid and pyrazinamide. The primary endpoint was sputum culture status at completion of 8 weeks of treatment.121 participants (56% of accrual target were enrolled. At completion of 8 weeks of treatment, negative cultures using Löwenstein-Jensen (LJ medium occurred in 47/60 (78% participants in the investigational arm vs. 43/51 (84%, p = 0.47 in the control arm; negative cultures using liquid medium occurred in 37/47 (79% in the investigational arm vs. 27/41 (66%, p = 0.23 in the control arm. Time to stable culture conversion was shorter for the investigational arm vs. the control arm using liquid culture medium (p = 0.03, but there was no difference using LJ medium. Median rifapentine area under the concentration-time curve (AUC0-24 was 313 mcg*h/mL, similar to recent studies of rifapentine dosed at 450-600 mg daily. Median moxifloxacin AUC0-24 was 28.0 mcg*h/mL, much lower than in trials where rifapentine was given only intermittently with moxifloxacin. The proportion of participants discontinuing assigned treatment for reasons other than microbiological ineligibility was higher in the investigational arm vs. the control arm (11/62 [18%] vs. 3/59 [5%], p = 0.04 although the proportions of grade 3 or higher adverse events were similar (5/62 [8%] in the

  14. A Phase 2 Randomized Trial of a Rifapentine plus Moxifloxacin-Based Regimen for Treatment of Pulmonary Tuberculosis

    Science.gov (United States)

    Conde, Marcus B.; Mello, Fernanda C. Q.; Duarte, Rafael Silva; Cavalcante, Solange C.; Rolla, Valeria; Dalcolmo, Margareth; Loredo, Carla; Durovni, Betina; Armstrong, Derek T.; Efron, Anne; Barnes, Grace L.; Marzinke, Mark A.; Savic, Radojka M.; Dooley, Kelly E.; Cohn, Silvia; Moulton, Lawrence H.; Chaisson, Richard E.; Dorman, Susan E.

    2016-01-01

    Background The combination of rifapentine and moxifloxacin administered daily with other anti-tuberculosis drugs is highly active in mouse models of tuberculosis chemotherapy. The objective of this phase 2 clinical trial was to determine the bactericidal activity, safety, and tolerability of a regimen comprised of rifapentine, moxifloxacin, isoniazid, and pyrazinamide administered daily during the first 8 weeks of pulmonary tuberculosis treatment. Methods Adults with sputum smear-positive pulmonary tuberculosis were randomized to receive either rifapentine (approximately 7.5 mg/kg) plus moxifloxacin (investigational arm), or rifampin (approximately 10 mg/kg) plus ethambutol (control) daily for 8 weeks, along with isoniazid and pyrazinamide. The primary endpoint was sputum culture status at completion of 8 weeks of treatment. Results 121 participants (56% of accrual target) were enrolled. At completion of 8 weeks of treatment, negative cultures using Löwenstein-Jensen (LJ) medium occurred in 47/60 (78%) participants in the investigational arm vs. 43/51 (84%, p = 0.47) in the control arm; negative cultures using liquid medium occurred in 37/47 (79%) in the investigational arm vs. 27/41 (66%, p = 0.23) in the control arm. Time to stable culture conversion was shorter for the investigational arm vs. the control arm using liquid culture medium (p = 0.03), but there was no difference using LJ medium. Median rifapentine area under the concentration-time curve (AUC0-24) was 313 mcg*h/mL, similar to recent studies of rifapentine dosed at 450–600 mg daily. Median moxifloxacin AUC0-24 was 28.0 mcg*h/mL, much lower than in trials where rifapentine was given only intermittently with moxifloxacin. The proportion of participants discontinuing assigned treatment for reasons other than microbiological ineligibility was higher in the investigational arm vs. the control arm (11/62 [18%] vs. 3/59 [5%], p = 0.04) although the proportions of grade 3 or higher adverse events were

  15. Moxifloxacin: Clinically compatible contrast agent for multiphoton imaging

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    Wang, Taejun; Jang, Won Hyuk; Lee, Seunghun; Yoon, Calvin J.; Lee, Jun Ho; Kim, Bumju; Hwang, Sekyu; Hong, Chun-Pyo; Yoon, Yeoreum; Lee, Gilgu; Le, Viet-Hoan; Bok, Seoyeon; Ahn, G.-One; Lee, Jaewook; Gho, Yong Song; Chung, Euiheon; Kim, Sungjee; Jang, Myoung Ho; Myung, Seung-Jae; Kim, Myoung Joon; So, Peter T. C.; Kim, Ki Hean

    2016-06-01

    Multiphoton microscopy (MPM) is a nonlinear fluorescence microscopic technique widely used for cellular imaging of thick tissues and live animals in biological studies. However, MPM application to human tissues is limited by weak endogenous fluorescence in tissue and cytotoxicity of exogenous probes. Herein, we describe the applications of moxifloxacin, an FDA-approved antibiotic, as a cell-labeling agent for MPM. Moxifloxacin has bright intrinsic multiphoton fluorescence, good tissue penetration and high intracellular concentration. MPM with moxifloxacin was demonstrated in various cell lines, and animal tissues of cornea, skin, small intestine and bladder. Clinical application is promising since imaging based on moxifloxacin labeling could be 10 times faster than imaging based on endogenous fluorescence.

  16. Evaluation of moxifloxacin-induced biochemical changes in mice

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    Grace E Ukpo

    2012-01-01

    Full Text Available The aim of the present study was to investigate the toxicological effects of moxifloxacin in mice to determine the toxicological implications. Forty mice of both sexes were divided into four groups of 10 mice each, designated as A, B, C and D. Group A served as the control and received 2 ml of distilled water, while Groups B, C and D were orally administered 12.5, 25 and 50 mg/kg body weight of moxifloxacin once daily for 7 days, respectively. The weights of the mice were recorded before and throughout the duration of drug administration. Blood samples were collected for serum analysis. Total blood protein, cholesterol, triglyceride, creatinine, activities of aspartate transaminase, alanine transaminase and alkaline phosphatase, levels of high density lipoprotein-cholesterol and low density lipoprotein-cholesterol were assayed. There were significant ( P≤0.05 differences in the concentrations of serum creatinine, urea, aspartate transaminase, alanine transaminase and alkaline phosphatase, levels of high density lipoprotein-cholesterol, low density lipoprotein-cholesterol, cholesterol and triglyceride of mice administered moxifloxacin. Serum level of total bilirubin in low dose treated animals was not significantly different from that of the control group animals, but there were significant dose dependent decrease in the animals treated with 25 mg/kg as well as 50 mg/kg. Data of the study indicate there was a dose dependent reduction in the protein metabolites, lipid profile and liver enzyme activities of mice administered moxifloxacin.

  17. Update on the Cardiac Safety of Moxifloxacin

    OpenAIRE

    Haverkamp, Wilhelm; Kruesmann, Frank; Fritsch, Anna; van Veenhuyzen, David; Arvis, Pierre

    2012-01-01

    Cardiac safety was compared in patients receiving moxifloxacin and other antimicrobials in a large patient population from Phase II–IV randomized active-controlled clinical trials. Moxifloxacin 400 mg once-daily monotherapy was administered orally (PO) or sequentially (intravenous/oral, IV/PO). Across 64 trials, 21,298 patients received PO therapy (10,613 moxifloxacin, 10,685 comparators) while 6846 received sequential IV/PO therapy (3431 moxifloxacin, 3415 comparators). Treatment-emergent ca...

  18. Moxifloxacin Induced Acute Delirium with Visual Hallucinations

    OpenAIRE

    Karagoz, Ergenekon; Ulcay, Asim; Budakli, Asil; Tutuncu, Recep

    2015-01-01

    AbstractA few reports accuse and implicate moxifloxacin as a contributor to delirium state.Here, we report the A 60-year-old female patient, who developed acute delirium with visual hallucinations following treatment with moxifloxacin for atypical pneumonia.   Key Words: Moxifloxacin, delirium, hallucination 

  19. Heterogeneous photocatalysis of moxifloxacin in water: chemical transformation and ecotoxicity.

    Science.gov (United States)

    Van Doorslaer, Xander; Haylamicheal, Israel Deneke; Dewulf, Jo; Van Langenhove, Herman; Janssen, Colin R; Demeestere, Kristof

    2015-01-01

    This work provides new insights on the impact of TiO2/UV catalyzed chemical transformation of moxifloxacin on ecotoxicity effects towards the green alga Pseudokirchneriella subcapitata. The moxifloxacin median effect concentration (EC-50=0.78 [0.56, 1.09] mg L(-1)), determined in accordance to the OECD 72-h growth inhibition test guideline, was 7 times lower than that of the older and widely used fluoroquinolone ciprofloxacin (EC-50=5.57 [4.86, 6.38] mg L(-1)). Applying heterogeneous photocatalysis as an advanced oxidation technique to degrade moxifloxacin in aqueous solution decreased the average growth inhibition from 72% to 14% after 150 min of treatment. No significant carbon mineralization was observed and liquid chromatography mass spectrometry analysis revealed the formation of 13 degradation products for which a chemical structure could be proposed based on accurate mass determination. Combined chemical and ecotoxicological analysis showed that as long as moxifloxacin is present in the reaction solution, it is the main compound affecting algal growth inhibition. However, also the contribution of the degradation products to the observed ecotoxicity cannot be neglected. Photocatalytically induced modifications of moxifloxacin mainly occur at the diazobicyclo-substituent as ring opening, oxidation into carbonyl groups, and hydroxylation. This results into the formation of more hydrophilic compounds with a decreased biological activity compared with moxifloxacin. The change in lipophilicity, and possibly a modified acid-base speciation, most probably also affect the cell membrane permeation of the degradation products, which might be another factor explaining the observed lower residual ecotoxicity of the photocatalytically treated reaction solutions. PMID:24735961

  20. Heterogeneous photocatalysis of moxifloxacin in water: chemical transformation and ecotoxicity.

    Science.gov (United States)

    Van Doorslaer, Xander; Haylamicheal, Israel Deneke; Dewulf, Jo; Van Langenhove, Herman; Janssen, Colin R; Demeestere, Kristof

    2015-01-01

    This work provides new insights on the impact of TiO2/UV catalyzed chemical transformation of moxifloxacin on ecotoxicity effects towards the green alga Pseudokirchneriella subcapitata. The moxifloxacin median effect concentration (EC-50=0.78 [0.56, 1.09] mg L(-1)), determined in accordance to the OECD 72-h growth inhibition test guideline, was 7 times lower than that of the older and widely used fluoroquinolone ciprofloxacin (EC-50=5.57 [4.86, 6.38] mg L(-1)). Applying heterogeneous photocatalysis as an advanced oxidation technique to degrade moxifloxacin in aqueous solution decreased the average growth inhibition from 72% to 14% after 150 min of treatment. No significant carbon mineralization was observed and liquid chromatography mass spectrometry analysis revealed the formation of 13 degradation products for which a chemical structure could be proposed based on accurate mass determination. Combined chemical and ecotoxicological analysis showed that as long as moxifloxacin is present in the reaction solution, it is the main compound affecting algal growth inhibition. However, also the contribution of the degradation products to the observed ecotoxicity cannot be neglected. Photocatalytically induced modifications of moxifloxacin mainly occur at the diazobicyclo-substituent as ring opening, oxidation into carbonyl groups, and hydroxylation. This results into the formation of more hydrophilic compounds with a decreased biological activity compared with moxifloxacin. The change in lipophilicity, and possibly a modified acid-base speciation, most probably also affect the cell membrane permeation of the degradation products, which might be another factor explaining the observed lower residual ecotoxicity of the photocatalytically treated reaction solutions.

  1. Moxifloxacin versus amoxicillin/clavulanic acid in outpatient acute exacerbations of COPD: MAESTRAL results.

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    Wilson, Robert; Anzueto, Antonio; Miravitlles, Marc; Arvis, Pierre; Alder, Jeff; Haverstock, Daniel; Trajanovic, Mila; Sethi, Sanjay

    2012-07-01

    Bacterial infections causing acute exacerbations of chronic obstructive pulmonary disease (AECOPD) frequently require antibacterial treatment. More evidence is needed to guide antibiotic choice. The Moxifloxacin in Acute Exacerbations of Chronic Bronchitis TriaL (MAESTRAL) was a multiregional, randomised, double-blind non-inferiority outpatient study. Patients were aged ≥ 60 yrs, with an Anthonisen type I exacerbation, a forced expiratory volume in 1 s clavulanic acid 875/125 mg p.o. b.i.d. (7 days). The primary end-point was clinical failure 8 weeks post-therapy in the per protocol population. Moxifloxacin was noninferior to amoxicillin/clavulanic acid at the primary end-point (111 (20.6%) out of 538, versus 114 (22.0%) out of 518, respectively; 95% CI -5.89-3.83%). In patients with confirmed bacterial AECOPD, moxifloxacin led to significantly lower clinical failure rates than amoxicillin/clavulanic acid (in the intent-to-treat with pathogens, 62 (19.0%) out of 327 versus 85 (25.4%) out of 335, respectively; p=0.016). Confirmed bacterial eradication at end of therapy was associated with higher clinical cure rates at 8 weeks post-therapy overall (p=0.0014) and for moxifloxacin (p=0.003). Patients treated with oral corticosteroids had more severe disease and higher failure rates. The MAESTRAL study showed that moxifloxacin was as effective as amoxicillin/clavulanic acid in the treatment of outpatients with AECOPD. Both therapies were well tolerated.

  2. Sustained Uptake of a Hospital-Based Handwashing with Soap and Water Treatment Intervention (Cholera-Hospital-Based Intervention for 7 Days [CHoBI7]): A Randomized Controlled Trial.

    Science.gov (United States)

    George, Christine Marie; Jung, Danielle S; Saif-Ur-Rahman, K M; Monira, Shirajum; Sack, David A; Mahamud-ur Rashid; Mahmud, Md Toslim; Mustafiz, Munshi; Rahman, Zillur; Bhuyian, Sazzadul Islam; Winch, Peter J; Leontsini, Elli; Perin, Jamie; Begum, Farzana; Zohura, Fatema; Biswas, Shwapon; Parvin, Tahmina; Sack, R Bradley; Alam, Munirul

    2016-02-01

    Diarrhea is the second leading cause of death in children under 5 years of age globally. The time patients and caregivers spend at a health facility for severe diarrhea presents the opportunity to deliver water, sanitation, and hygiene (WASH) interventions. We recently developed Cholera-Hospital-Based Intervention for 7 days (CHoBI7), a 1-week hospital-based handwashing with soap and water treatment intervention, for household members of cholera patients. To investigate if this intervention could lead to sustained WASH practices, we conducted a follow-up evaluation of 196 intervention household members and 205 control household members enrolled in a randomized controlled trial of the CHoBI7 intervention 6 to 12 months post-intervention. Compared with the control arm, the intervention arm had four times higher odds of household members' handwashing with soap at a key time during 5-hour structured observation (odds ratio [OR]: 4.71, 95% confidence interval [CI]: 2.61, 8.49) (18% versus 50%) and a 41% reduction in households in the World Health Organization very high-risk category for stored drinking water (OR: 0.38, 95% CI: 0.15, 0.96) (58% versus 34%) 6 to 12 months post-intervention. Furthemore, 71% of observed handwashing with soap events in the intervention arm involved the preparation and use of soapy water, which was promoted during the intervention, compared to 9% of control households. These findings demonstrate that the hospital-based CHoBI7 intervention can lead to significant increases in handwashing with soap practices and improved stored drinking water quality 6 to 12 months post-intervention. PMID:26728766

  3. Sustained Uptake of a Hospital-Based Handwashing with Soap and Water Treatment Intervention (Cholera-Hospital-Based Intervention for 7 Days [CHoBI7]): A Randomized Controlled Trial.

    Science.gov (United States)

    George, Christine Marie; Jung, Danielle S; Saif-Ur-Rahman, K M; Monira, Shirajum; Sack, David A; Mahamud-ur Rashid; Mahmud, Md Toslim; Mustafiz, Munshi; Rahman, Zillur; Bhuyian, Sazzadul Islam; Winch, Peter J; Leontsini, Elli; Perin, Jamie; Begum, Farzana; Zohura, Fatema; Biswas, Shwapon; Parvin, Tahmina; Sack, R Bradley; Alam, Munirul

    2016-02-01

    Diarrhea is the second leading cause of death in children under 5 years of age globally. The time patients and caregivers spend at a health facility for severe diarrhea presents the opportunity to deliver water, sanitation, and hygiene (WASH) interventions. We recently developed Cholera-Hospital-Based Intervention for 7 days (CHoBI7), a 1-week hospital-based handwashing with soap and water treatment intervention, for household members of cholera patients. To investigate if this intervention could lead to sustained WASH practices, we conducted a follow-up evaluation of 196 intervention household members and 205 control household members enrolled in a randomized controlled trial of the CHoBI7 intervention 6 to 12 months post-intervention. Compared with the control arm, the intervention arm had four times higher odds of household members' handwashing with soap at a key time during 5-hour structured observation (odds ratio [OR]: 4.71, 95% confidence interval [CI]: 2.61, 8.49) (18% versus 50%) and a 41% reduction in households in the World Health Organization very high-risk category for stored drinking water (OR: 0.38, 95% CI: 0.15, 0.96) (58% versus 34%) 6 to 12 months post-intervention. Furthemore, 71% of observed handwashing with soap events in the intervention arm involved the preparation and use of soapy water, which was promoted during the intervention, compared to 9% of control households. These findings demonstrate that the hospital-based CHoBI7 intervention can lead to significant increases in handwashing with soap practices and improved stored drinking water quality 6 to 12 months post-intervention.

  4. Moxifloxacin Induced Seizures -A Case Report.

    Directory of Open Access Journals (Sweden)

    Jiana Shi

    2014-09-01

    Full Text Available A 73-year-old female patient developed a generalized tonic-clonic seizure on the 6th day after treatment with moxifloxacin 400 mg daily intravenously for appendicitis. This patient had atrial fibrillation and history of a surgery for intracerebral hemorrhage, with impaired renal function and liver function, but without history of seizures. Moxifloxacin was discontinued and switched to cefuroxime. The patient remained seizure-free at discharge four days later. The naranjo adverse drug reaction probability scale score was 4, indicating a possible adverse reaction to moxifloxacin. The potential risk factors related to moxifloxacin-induced seizures are discussed. It highlights that preexisting central nervous system disease, elderly female with lower bodyweight and severe renal impairment may be the risk factors involved in moxifloxacin-induced seizures.

  5. In vivo 3D measurement of moxifloxacin and gatifloxacin distributions in the mouse cornea using multiphoton microscopy

    Science.gov (United States)

    Lee, Seunghun; Lee, Jun Ho; Park, Jin Hyoung; Yoon, Yeoreum; Chung, Wan Kyun; Tchah, Hungwon; Kim, Myoung Joon; Kim, Ki Hean

    2016-05-01

    Moxifloxacin and gatifloxacin are fourth-generation fluoroquinolone antibiotics used in the clinic to prevent or treat ocular infections. Their pharmacokinetics in the cornea is usually measured from extracted ocular fluids or tissues, and in vivo direct measurement is difficult. In this study multiphoton microscopy (MPM), which is a 3D optical microscopic technique based on multiphoton fluorescence, was applied to the measurement of moxifloxacin and gatifloxacin distribution in the cornea. Intrinsic multiphoton fluorescence properties of moxifloxacin and gatifloxacin were characterized, and their distributions in mouse cornea in vivo were measured by 3D MPM imaging. Both moxifloxacin and gatifloxacin had similar multiphoton spectra, while moxifloxacin had stronger fluorescence than gatifloxacin. MPM imaging of mouse cornea in vivo showed (1) moxifloxacin had good penetration through the superficial corneal epithelium, while gatifloxacin had relatively poor penetration, (2) both ophthalmic solutions had high intracellular distribution. In vivo MPM results were consistent with previous studies. This study demonstrates the feasibility of MPM as a method for in vivo direct measurement of moxifloxacin and gatifloxacin in the cornea.

  6. Moxifloxacin Induced Seizures -A Case Report

    OpenAIRE

    Jiana Shi; Huimin Xu

    2014-01-01

    Abstract A 73-year-old female patient developed a generalized tonic-clonic seizure on the 6th day after treatment with moxifloxacin 400 mg daily intravenously for appendicitis. This patient had atrial fibrillation and history of a surgery for intracerebral hemorrhage, with impaired renal function and liver function, but without history of seizures. Moxifloxacin was discontinued and switched to cefuroxime. The patient remained seizure-free at discharge four days later. The naranjo adverse drug...

  7. Powerful Bactericidal Activity of Moxifloxacin in Human Leprosy▿

    OpenAIRE

    Pardillo, Fe Eleanor F.; Burgos, Jasmin; Fajardo, Tranquilino T.; Cruz, Eduardo Dela; Abalos, Rodolfo M.; Paredes, Rose Maria D.; Andaya, Cora Evelyn S.; Gelber, Robert H.

    2008-01-01

    In a clinical trial of moxifloxacin in eight multibacillary leprosy patients, moxifloxacin proved highly effective. In all trial patients, a single 400-mg dose of moxifloxacin resulted in significant killing (P ≤ 0.006) of Mycobacterium leprae, ranging from 82% to 99%, with a mean of 91%. In all instances, no viable bacilli were detected with an additional 3 weeks of daily therapy, this observed rapid bactericidal activity being matched previously only by rifampin. On moxifloxacin therapy, sk...

  8. Bacteriostatic and Bactericidal Activities of Moxifloxacin against Coxiella burnetii

    OpenAIRE

    Rolain, Jean-Marc; Maurin, Max; Raoult, Didier

    2001-01-01

    The in vitro activity of moxifloxacin against Coxiella burnetii was compared to those of pefloxacin, ofloxacin, and doxycycline. MICs of moxifloxacin ranged from 0.5 to 1 μg/ml for the Nine Mile, Priscilla, and Q212 strains. Moxifloxacin was not bactericidal against C. burnetii at 4 μg/ml.

  9. In vivo 3D measurement of moxifloxacin and gatifloxacin distributions in the mouse cornea using multiphoton microscopy

    OpenAIRE

    Seunghun Lee; Jun Ho Lee; Jin Hyoung Park; Yeoreum Yoon; Wan Kyun Chung; Hungwon Tchah; Myoung Joon Kim; Ki Hean Kim

    2016-01-01

    Moxifloxacin and gatifloxacin are fourth-generation fluoroquinolone antibiotics used in the clinic to prevent or treat ocular infections. Their pharmacokinetics in the cornea is usually measured from extracted ocular fluids or tissues, and in vivo direct measurement is difficult. In this study multiphoton microscopy (MPM), which is a 3D optical microscopic technique based on multiphoton fluorescence, was applied to the measurement of moxifloxacin and gatifloxacin distribution in the cornea. I...

  10. Severe QT interval prolongation associated with moxifloxacin: a case report

    OpenAIRE

    Koide, Tetsuro; Shiba, Masato; Tanaka, Katsuhiro; Muramatsu, Masatoshi; Ishida, Satoshi; Kondo, Yoshihiro; Watanabe, Keiko

    2008-01-01

    Introduction The QT interval prolongation is an adverse effect associated with moxifloxacin. This adverse effect can lead to potentially life-threatening arrhythmias such as Torsades de pointes. We describe a case of severe QT interval prolongation associated with moxifloxacin which may cause the development of Torsades de pointes. There have been no reported case of severe corrected QT interval prolongation caused by moxifloxacin in the patient of normal heart rate. Case presentation In an 8...

  11. Rifampicin reduces plasma concentrations of moxifloxacin in patients with tuberculosis.

    OpenAIRE

    Nijland, H.M.J.; Ruslami, R.; Suroto, A.J.; Burger, D. M.; Alisjahbana, B.; van Crevel, R.; Aarnoutse, R E

    2007-01-01

    BACKGROUND: The long duration of the current tuberculosis (TB) treatment is demanding and warrants the development of new drugs. Moxifloxacin shows promising results and may be combined with rifampicin to shorten the duration of TB treatment. Rifampicin induces the phase II metabolic enzymes that are involved in the biotransformation of moxifloxacin. Therefore, the interaction between rifampicin and moxifloxacin should be investigated. PATIENTS AND METHODS: Nineteen Indonesian patients with p...

  12. HERG Protein Plays a Role in Moxifloxacin-Induced Hypoglycemia

    OpenAIRE

    Hai-Yan Qiu; Sha-Sha Yuan; Fang-Yuan Yang; Ting-Ting Shi; Jin-Kui Yang

    2016-01-01

    The purpose of this study was to investigate the effect of moxifloxacin on HERG channel protein and glucose metabolism. HERG expression was investigated using immunohistochemistry. The whole-cell patch clamp method was used to examine the effect of moxifloxacin on HERG channel currents. A glucose tolerance test was used to analyze the effects of moxifloxacin on blood glucose and insulin concentrations in mice. Results show that HERG protein was expressed in human pancreatic β-cells. Moxifloxa...

  13. Penetration of Moxifloxacin into Peripheral Compartments in Humans

    OpenAIRE

    Müller, Markus; Staß, Heino; Brunner, Martin; Möller, Jan G.; Lackner, Edith; Eichler, Hans G.

    1999-01-01

    To characterize the penetration of moxifloxacin (BAY 12-8039) into peripheral target sites, the present study aimed at measuring unbound moxifloxacin concentrations in the interstitial space fluid by means of microdialysis, an innovative clinical sampling technique. In addition, moxifloxacin concentrations were measured in cantharides-induced skin blisters, saliva, and capillary plasma and compared to total- and free-drug concentrations in venous plasma. For this purpose, 12 healthy volunteer...

  14. Chitosan gel-embedded moxifloxacin niosomes: An efficient antimicrobial hybrid system for burn infection.

    Science.gov (United States)

    Sohrabi, Shohreh; Haeri, Azadeh; Mahboubi, Arash; Mortazavi, Alireza; Dadashzadeh, Simin

    2016-04-01

    The purpose of this study was to prepare and characterize a hybrid system of moxifloxacin loaded niosomes incorporated into chitosan gel as a potential carrier for topical antimicrobial delivery. The prepared system was characterized regarding entrapment efficiency, particle size, zeta potential, in vitro drug release kinetics, morphology, FTIR analysis, bioadhesive strength and rheological behavior. The effect of different formulation parameters (surfactant type, surfactant to drug ratio, cholesterol percentage and loading methodology) on moxifloxacin entrapment and drug release was evaluated. The antibacterial effectiveness of various formulations was also assessed by measuring the minimal inhibitory concentrations, minimal bactericidal concentrations and agar diffusion assay using Pseudomonas aeruginosa and Staphylococcus aureus as model pathogens. The optimized niosomal formulation showed 73% drug entrapment, 47% drug release in 8h and was ∼290 nm in particle diameter and negatively charged (ζ∼-23 mV). The gel-embedded niosomes exhibited pseudo-plastic flow behavior and more sustained drug release profile compared to niosomes. The niosomal formulation of moxifloxacin was the most efficient system against P. aeruginosa, while gel based formulations were superior against S. aureus. Taken together, moxifloxacin-in-niosomes-in-gels hold great promise for topical microbial infections. PMID:26794314

  15. Fuel utilization during exercise after 7 days of bed rest

    Science.gov (United States)

    Barrows, Linda H.; Harris, Bernard A.; Moore, Alan D.; Siconolfi, Steven F.

    1992-01-01

    Energy yield from carbohydrate, fat, and protein during physical activity is partially dependent on an individual's fitness level. Prolonged exposure to microgravity causes musculoskeletal and cardiovascular deconditioning; these adaptations may alter fuel utilization during space flight. Carbohydrate and fat metabolism during exercise were analyzed before and after 7 days of horizontal bed rest.

  16. Moxifloxacin (Avelox) Induced Thrombotic Thrombocytopenic Purpura

    OpenAIRE

    Surana, Sikander P.; Zahily Sardinas; Multz, Alan S.

    2012-01-01

    We report a case of a 66-year-old African-American female who presented with complaints of progressively worsening weakness, shortness of breath on minimal exertion, lethargy for the last few days, and short episodes of aphasia lasting 20–30 seconds. Prior to presentation, she was treated with two courses of moxifloxacin for sinusitis. Laboratory examination was remarkable for anemia and thrombocytopenia with elevated lactate dehydrogenase and no evidence of renal failure. Peripheral smear sh...

  17. Pharmacokinetics of Moxifloxacin in an Infant with Mycoplasma hominis Meningitis

    OpenAIRE

    Watt, Kevin M; Massaro, Matthew M; Smith, Brian; Cohen-Wolkowiez, Michael; Benjamin, Daniel K; Laughon, Matthew M

    2012-01-01

    Treatment of Mycoplasma hominis meningitis in infants is limited by a lack of consensus regarding therapy and limited pharmacokinetic data for agents to which M. hominis is susceptible. We report the successful treatment of a premature infant with M. hominis meningitis with doxycycline and moxifloxacin and provide a pharmacokinetic profile of moxifloxacin.

  18. Moxifloxacin Prophylaxis against MDR TB, New York, New York, USA

    OpenAIRE

    Trieu, Lisa; Proops, Douglas C.; Shama D. Ahuja

    2015-01-01

    Contacts of persons infected with multidrug-resistant tuberculosis (MDR TB) have few prophylaxis options. Of 50 contacts of HIV- and MDR TB–positive persons who were treated with moxifloxacin, 30 completed treatment and 3 discontinued treatment because of gastrointestinal symptoms. Moxifloxacin was generally well-tolerated; further research of its efficacy against MDR TB is needed.

  19. IR Camera Report for the 7 Day Production Test

    Energy Technology Data Exchange (ETDEWEB)

    Holloway, Michael Andrew [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-02-22

    The following report gives a summary of the IR camera performance results and data for the 7 day production run that occurred from 10 Sep 2015 thru 16 Sep 2015. During this production run our goal was to see how well the camera performed its task of monitoring the target window temperature with our improved alignment procedure and emissivity measurements. We also wanted to see if the increased shielding would be effective in protecting the camera from damage and failure.

  20. IR Camera Report for the 7 Day Production Test

    International Nuclear Information System (INIS)

    The following report gives a summary of the IR camera performance results and data for the 7 day production run that occurred from 10 Sep 2015 thru 16 Sep 2015. During this production run our goal was to see how well the camera performed its task of monitoring the target window temperature with our improved alignment procedure and emissivity measurements. We also wanted to see if the increased shielding would be effective in protecting the camera from damage and failure.

  1. Plasma Disposition of Conventional and Long-Acting Moxifloxacin in Sheep after Intravenous Administration

    OpenAIRE

    C. M. Modi; S. K. Mody; Modi, F. D.; Patel, H. B.

    2012-01-01

    This study describes disposition of long-acting moxifloxacin and conventional formulations of moxifloxacin in sheep after intravenous administration in five male sheep. Long acting moxifloxacin solution (10% moxifloxacin in solution with L-arginine, N-butyl alcohol, and benzyl alcohol) and conventional moxifloxacin (10%) were injected in jugular vein. Blood samples were collected from contralateral jugular vein in test tubes containing 30–50 IU heparin (anticoagulant) periodically from 0.083 ...

  2. Moxifloxacin in the Therapy of Experimental Pneumococcal Meningitis

    OpenAIRE

    Schmidt, H.; Dalhoff, A.; Stuertz, K; Trostdorf, F.; Chen, V.; Schneider, O.; Kohlsdorfer, C.; BRÜCK, W.; Nau, R.

    1998-01-01

    The activity of moxifloxacin (BAY 12-8039) against a Streptococcus pneumoniae type 3 strain (MIC and minimum bactericidal concentration [MBC] of moxifloxacin, 0.06 and 0.25 μg/ml, respectively; MIC and MBC of ceftriaxone, 0.03 and 0.06 μg/ml, respectively) was determined in vitro and in a rabbit model of meningitis. Despite comparable bactericidal activity, 10 μg of moxifloxacin per ml released lipoteichoic and teichoic acids less rapidly than 10 μg of ceftriaxone per ml in vitro. Against exp...

  3. Drug-induced immune thrombocytopenia due to moxifloxacin

    OpenAIRE

    Coker, Timothy J

    2013-01-01

    A 39-year-old woman with 1 day of oral petechiae, leg ecchymoses and epistaxis was found to have isolated thrombocytopenia. She had recently completed a 10-day course of moxifloxacin for an upper respiratory infection. On further questioning, she had developed thrombocytopenia 2 years earlier after a treatment course with moxifloxacin. After ruling out other causes, drug-induced immune thrombocytopenia due to moxifloxacin was diagnosed. Her platelets returned to normal range 15 days after fin...

  4. The role of moxifloxacin in tuberculosis therapy

    Directory of Open Access Journals (Sweden)

    Stephen H. Gillespie

    2016-03-01

    Full Text Available Tuberculosis (TB remains a global threat with more than 9 million new infections. Treatment remains difficult and there has been no change in the duration of the standard regimen since the early 1980s. Moreover, many patients are unable to tolerate this treatment and discontinue therapy, increasing the risk of resistance. There is a growing tide of multidrug resistance and few effective antibiotics to tackle the problem. Since the turn of the millennium there has been a surge in interest in developing new therapies for TB and a number of new drugs have been developed. In this review the repurposing of moxifloxacin, an 8-methoxy-fluoroquinolone, for TB treatment is discussed. The evidence that underpins the development of this agent is reviewed. The results of the recently completed phase III trials are summarised and the reasons for the unexpected outcome are explored. Finally, the design of new trials that incorporate moxifloxacin, and that address both susceptible disease and multidrug resistance, is described.

  5. Efficacy and safety of moxifloxacin in acute exacerbations of chronic bronchitis: a prospective, multicenter, observational study (AVANTI

    Directory of Open Access Journals (Sweden)

    Chuchalin Alexander

    2013-01-01

    Full Text Available Abstract Background Acute exacerbations of chronic bronchitis (AECB, including chronic obstructive pulmonary disease (AECOPD, represent a substantial patient burden. Few data exist on outpatient antibiotic management for AECB/AECOPD in Eastern/South Eastern Europe, in particular on the use of moxifloxacin (Avelox®, although moxifloxacin is widely approved in this region based on evidence from international clinical studies. Methods AVANTI (AVelox® in Acute Exacerbations of chroNic bronchiTIs was a prospective, observational study conducted in eight Eastern European countries in patients > 35 years with AECB/AECOPD to whom moxifloxacin was prescribed. In addition to safety and efficacy outcomes, data on risk factors and the impact of exacerbation on daily life were collected. Results In the efficacy population (N = 2536, chronic bronchitis had been prevalent for > 10 years in 31.4% of patients and 66.0% of patients had concomitant COPD. Almost half the patients had never smoked, in contrast to data from Western Europe and the USA, where only one-quarter of COPD patients are non-smokers. The mean number of exacerbations in the last 12 months was 2.7 and 26.3% of patients had been hospitalized at least once for exacerbation. Physician compliance with the recommended moxifloxacin dose (400 mg once daily was 99.6%. The mean duration of moxifloxacin therapy for the current exacerbation (Anthonisen type I or II in 83.1%; predominantly type I was 6.4 ± 1.9 days. Symptom improvement was reported after a mean of 3.4 ± 1.4 days. After 5 days, 93.2% of patients reported improvement and, in total, 93.5% of patients were symptom-free after 10 days. In the safety population (N = 2672, 57 (2.3% patients had treatment-emergent adverse events (TEAEs and 4 (0.15% had serious TEAEs; no deaths occurred. These results are in line with the known safety profile of moxifloxacin. Conclusions A significant number of patients in this

  6. Cohort Study of Intracameral Moxifloxacin in Postoperative Endophthalmitis Prophylaxis

    OpenAIRE

    Virgilio Galvis; Alejandro Tello; Mary Alejandra Sánchez; Paul Anthony Camacho

    2014-01-01

    We conducted a cohort study to evaluate post-cataract surgery endophthalmitis rates in relation to prophylactic intracameral moxifloxacin administration. A total of 2332 patients (2674 eyes) who underwent phacoemulsification by a single surgeon from January 2007 through December 2012 were included in the study. A total of 1056 eyes did not receive intracameral prophylactic moxifloxacin and the antibiotic was injected in 1618 eyes. The incidence of presumed postoperative endophthalmitis in the...

  7. Moxifloxacin in the treatment of skin and skin structure infections

    OpenAIRE

    Guay, David RP

    2006-01-01

    Moxifloxacin is a recent addition to the fluoroquinolone class, differing from ciprofloxacin and other older agents in having much better in vitro activity against Gram-positive aerobes while retaining potent activity against Gram-negative aerobes. It is also active against the pathogens of human and animal bite wounds and those species of atypical mycobacteria associated with dermatologic infections. Its activity against anaerobes is quite variable. Moxifloxacin penetrates well into inflamma...

  8. Moxifloxacin: Clinically compatible contrast agent for multiphoton imaging

    OpenAIRE

    Taejun Wang; Won Hyuk Jang; Seunghun Lee; Yoon, Calvin J.; Jun Ho Lee; Bumju Kim; Sekyu Hwang; Chun-Pyo Hong; Yeoreum Yoon; Gilgu Lee; Viet-Hoan Le; Seoyeon Bok; G-One Ahn; Jaewook Lee; Yong Song Gho

    2016-01-01

    Multiphoton microscopy (MPM) is a nonlinear fluorescence microscopic technique widely used for cellular imaging of thick tissues and live animals in biological studies. However, MPM application to human tissues is limited by weak endogenous fluorescence in tissue and cytotoxicity of exogenous probes. Herein, we describe the applications of moxifloxacin, an FDA-approved antibiotic, as a cell-labeling agent for MPM. Moxifloxacin has bright intrinsic multiphoton fluorescence, good tissue penetra...

  9. Safety of undiluted intracameral moxifloxacin without postoperative topical antibiotics in cataract surgery.

    Science.gov (United States)

    Zhou, Andrew Xingyu; Messenger, Wyatt Boyer; Sargent, Steven; Ambati, Balamurali Krishna

    2016-08-01

    The objective of this study is to evaluate the safety of undiluted 0.5 % intracameral moxifloxacin for postoperative endophthalmitis prophylaxis in cataract surgery patients without the use of additional postoperative topical antibiotics. All phacoemulsification cataract surgeries performed by a single surgeon (B.A.) at the John A. Moran Eye Center from June 2012 to May 2015 were reviewed retrospectively. From June 2012 to April 2014, patients were given topical 0.5 % moxifloxacin postoperatively. From May 2014 to May 2015, all patients were given moxifloxacin intracamerally with no antibiotics postoperatively. The follow-up period was 1 month after surgery. Preoperative visual acuity and postoperative visual acuity, corneal edema, and anterior chamber reaction were recorded and compared between the two groups. 384 cataract surgeries were performed during the study period. None of the 384 eyes in the study developed endophthalmitis. Of those 384 eyes, 222 were included in the study for analysis based on the inclusion and exclusion criteria. 131 were part of the topical antibiotic group and 91 were part of the intracameral group. The differences in uncorrected visual acuity at 1 day postoperatively (p = 0.595) and best corrected visual acuity at 1 month postoperatively (p = 0.099) were not statistically significant. Differences in corneal edema (p = 0.370) and anterior chamber reaction (p = 0.069) at 1 day postoperatively and corneal edema (p = 0.512) and anterior chamber reaction (p = 0.512) at 1 month postoperatively were also not statistically significant. Undiluted 0.5 % moxifloxacin can be safely injected intracamerally following cataract surgery without additional postoperative antibiotic prophylaxis to prevent endophthalmitis without adverse effects on patient outcomes. PMID:26577588

  10. A comparison of 5 or 7 days of rabeprazole triple therapy for eradication of Helicobacter pylori

    Directory of Open Access Journals (Sweden)

    Ari F. Syam

    2010-05-01

    Full Text Available Aim A combination of PPI and 1000 mg amoxicillin/500 mg clarithromycin twice daily for 2 weeks has been proven effective in the eradication of H. pylori. Most studies suggested that treatment for 7 and 10 days may be equally effective. Few data are available on the efficacy of 5-day triple therapy. Aim of this study was to compare 5-day and 7-day rabeprazole triple therapy for eradication of H. pylori infection.Methods We prospectively studied 60 consecutive H. pylori-infected patients who came to hospitals in six centres in Indonesia and who underwent upper endoscopy and biopsy. H. pylori infection was confirmed if two rapid urease tests (Pronto Dry and histology or urea breath test were positive. Patients were assigned to either an open-labelled 5-day or 7-day course of oral amoxicillin 1000 mg b.i.d., clarithromycin 500 mg b.i.d., and rabeprazole 10 mg b.i.d. (RAC. Four weeks after therapy, all patients had a repeated UBT for evaluation of the presence of H. pylori.Results Of the 60 patients (42 males and 18 females with mean age (± SD 47.63 ± 13.93 years, range 21–74 years, 25 patients (41.7% had 5-day treatment and 35 patients (58.3% had 7-day treatment. With 5-day treatment, 18 patients (72% and with 7-day treatment 32 patients (91.4% became negative for H. pylori infection. The eradication failure was found on 7 patients (28.0% in 5-day treatment and 3 patients (8.6% in 7-day treatment.Conclusions The study showed that the eradication of H. pylori infection by triple rabeprazole-based treatment in 7-day is still better than in 5-day. (Med J Indones 2010; 19:113-7Keywords: H. pylori, rabeprazole, triple therapy

  11. In Vitro Activities of Moxifloxacin, 127I-Moxifloxacin and 131I-Moxifloxacin Against Staphylococcus Aureus Biofilms

    Directory of Open Access Journals (Sweden)

    Hasan Demiroğlu

    2015-02-01

    Full Text Available Objective: The aim of the study was to investigate the antimicrobial effect of Moxifloxacin (MXF, radio (Na131I and cold (K127I iodinated MXF on methicillin susceptible Staphylococcus aureus ATCC 35556 (MSSA biofilms. Methods: MXF was labeled with Na131I using the iodogen method. The optimum radiodination conditions for 131I-MXF was determined by thin-layer radio chromatography studies. Thin-layer radio chromatography (TLRC chromatograms were obtained by using Cyclone Plus Storage Phosphor System. The MICs of MXF, 127I-MXF and 131I-MXF were determined using the microdilution broth method according to CLSI criteria. Time kill curves were performed over 24 h using an inoculum of 2×105 (CFU/mL. Biofilms were grown in microtitre plates, dyed with crystal violet and the mean optical density (OD630 was used for quantification. Biofilms were incubated MXF, 127I-MXF and 131I-MXF at various concentration (0.03 to 64 µg/mL. Results: MXF was labeled with 131I iodogen method. 131I-MXF was obtained with high a yield 95±3%. The MIC values for MXF, 127I-MXF and 131I-MXF was 0.06 µg/mL. Bactericidal activity was demonstrated at 0.25 µg/mL 4 hour for MXF, 127I-MXF and 131I-MXF. At MIC levels, MXF, 127I-MXF and 131I-MXF was not showed a marked reduction of metabolic activity in the S. aureus biofilm. The ODs of biofilm after incubation with an increasing antibiotic concentration were significantly lower than the ODs of biofilms without antibiotic p≤005. The radiolabeled MXF was most effective than MXF, 127I-MXF in reducing the number of bacteria in biofilm. After 24 h incubation Log10 CFU/mL values for 32 µg/mL antibiotic concentration: Control, MXF, 127I-MXF and 131I-MXF were 9.5, 4.3, 4.8 and 3.1, respectively. Conclusion: 131I and 127I were used alone there was no penetration of the S. aureus biofilm and no damage. In contrast our results demonstrate that the radiolabeled Moxifloxacin (131I-MXF have potent anti-biofilm activity against S. aureus

  12. Pharmacokinetics and Safety of Moxifloxacin in Children With Multidrug-Resistant Tuberculosis

    OpenAIRE

    Thee, Stephanie; Anthony J Garcia-Prats; Draper, Heather R.; McIlleron, Helen M.; Wiesner, Lubbe; Castel, Sandra; Schaaf, H Simon; Hesseling, Anneke C

    2014-01-01

    Moxifloxacin serum concentrations in children receiving multidrug-resistant tuberculosis treatment following an oral dose of 10 mg/kg were low. Human immunodeficiency virus infection was associated with lower moxifloxacin exposure. Moxifloxacin was generally well tolerated when taken for several months.

  13. Moxifloxacin Penetration in Bronchial Secretions of Mechanically Ventilated Patients with Pneumonia

    OpenAIRE

    Leone, Marc; Albanèse, Jacques; Sampol-Manos, Emmanuelle; Simon, Nicolas; Lacarelle, Bruno; Bruguerolle, Bernard; Martin, Claude

    2004-01-01

    The pharmacokinetics of moxifloxacin was studied in 17 mechanically ventilated patients with pneumonia. Patients were given 400 mg of moxifloxacin intravenously. Blood samples and bronchial secretions were taken on days 1 and 4. A dose of 400 mg of moxifloxacin allows one to achieve efficient concentrations in bronchial secretions and plasma.

  14. Moxifloxacin Population Pharmacokinetics in Patients with Pulmonary Tuberculosis and the Effect of Intermittent High-Dose Rifapentine

    OpenAIRE

    Zvada, Simbarashe P.; Denti, Paolo; Geldenhuys, Hennie; Meredith, Sandra; van As, Danelle; Hatherill, Mark; Hanekom, Willem; Wiesner, Lubbe; Simonsson, Ulrika S. H.; Jindani, Amina; Harrison, Thomas; McIlleron, Helen M.

    2012-01-01

    We described the population pharmacokinetics of moxifloxacin and the effect of high-dose intermittent rifapentine in patients with pulmonary tuberculosis who were randomized to a continuation-phase regimen of 400 mg moxifloxacin and 900 mg rifapentine twice weekly or 400 mg moxifloxacin and 1,200 mg rifapentine once weekly. A two-compartment model with transit absorption best described moxifloxacin pharmacokinetics. Although rifapentine increased the clearance of moxifloxacin by 8% during ant...

  15. Intraocular penetration of sequentially instilled topical moxifloxacin, gatifloxacin, and levofloxacin

    Directory of Open Access Journals (Sweden)

    Koji Sugioka

    2009-10-01

    Full Text Available Koji Sugioka1, Masahiko Fukuda1, Shohei Komoto1, Motoki Itahashi1, Masakazu Yamada2, Yoshikazu Shimomura11Department of Ophthalmology, Kinki University School of Medicine, Osaka-Sayama City, Osaka, Japan; 2Division for Vision Research, National Institute of Sensory Organs, National Tokyo Medical Center, Tokyo, JapanPurpose: The objective of the study was to compare the intraocular penetration levels of the newer fluoroquinolones, moxifloxacin, gatifloxacin, and levofloxacin in the rabbit’s cornea, aqueous humor, and conjunctiva after topical instillation.Methods: 0.5% moxifloxacin, 0.3% gatifloxacin, and 0.5% levofloxacin were instilled in random sequence in both eyes of nine New Zealand White rabbits at two-minute intervals. Instillation was repeated every 15 minutes for a total of three drops of each fluoroquinolone per eye. Three additional animals had only moxifloxacin instilled bilaterally using the same schedule. Sixty minutes after the final instillation, the rabbits were sacrificed for determination of corneal, aqueous humor, and conjunctival fluoroquinolone concentrations using highperformance liquid chromatography.Results: Moxifloxacin achieved significantly higher concentrations than levofloxacin and gatifloxacin in the cornea (P = 0.0102 and P = 0.0006, respectively, aqueous humor (P = 0.0015 and P < 0.0001, respectively, and conjunctiva (P = 0.0191 and P = 0.0236, respectively. Conclusions: 0.5% moxifloxacin eyedrops provided superior intraocular penetration in rabbits’ eyes compared with the two other fluoroquinolones, 0.5% levofloxacin and 0.3% gatifloxacin.Keywords: fluoroquinolone, gatifloxacin, levofloxacin, moxifloxacin, penetration, rabbit

  16. Zabofloxacin versus moxifloxacin in patients with COPD exacerbation: a multicenter, double-blind, double-dummy, randomized, controlled, Phase III, non-inferiority trial

    Directory of Open Access Journals (Sweden)

    Rhee CK

    2015-10-01

    -inferiority clinical trial designed to compare oral zabofloxacin (367 mg once daily for 5 days with moxifloxacin (400 mg once daily for 7 days for the treatment of patients with COPD exacerbation. In all, 345 COPD patients with a moderate COPD exacerbation were enrolled in the study via the outpatient clinics at 31 university hospitals. Clinical per protocol analysis revealed that the clinical cure rate for zabofloxacin was 86.7% and that for moxifloxacin was 86.3% (the rate difference, 0.4%; 95% confidence interval, –7.7%–8.6%. Intention-to-treat analysis revealed clinical cure rates of 77.1% and 77.3% (difference, –0.2%; 95% confidence interval, –9.0%–8.8%, respectively. These results confirm that zabofloxacin is not inferior to moxifloxacin. The favorable microbiological response rate for zabofloxacin was 67.4% and that for moxifloxacin was 79.5% (P=0.22. Patients in the zabofloxacin group showed better patient-oriented outcomes, as measured by EXAcerbations of Chronic Pulmonary Disease Tool-Patient-Reported Outcome and the COPD assessment test scores, than patients in the moxifloxacin group. Adverse drug reactions related to zabofloxacin occurred in 9.7% of cases and those related to moxifloxacin occurred in 9.6% of cases (P=0.97. The dropout rate due to adverse events was 0% (0/175 in the zabofloxacin group and 1.8% (3/167 in the moxifloxacin group (P=0.12. Oral zabofloxacin (367 mg once daily for 5 days was not inferior to oral moxifloxacin (400 mg once daily for 7 days for the treatment of patients with COPD exacerbation. Keywords: chronic obstructive pulmonary disease, exacerbation, zabofloxacin, quinolone, EXACT-PRO, CAT

  17. A Faropenem, Linezolid, and Moxifloxacin Regimen for Both Drug-Susceptible and Multidrug-Resistant Tuberculosis in Children: FLAME Path on the Milky Way

    Science.gov (United States)

    Deshpande, Devyani; Srivastava, Shashikant; Nuermberger, Eric; Pasipanodya, Jotam G.; Swaminathan, Soumya; Gumbo, Tawanda

    2016-01-01

    Background. The regimen of linezolid and moxifloxacin was found to be efficacious in the hollow fiber system model of pediatric intracellular tuberculosis. However, its kill rate was slower than the standard 3-drug regimen of isoniazid, rifampin, and pyrazinamide. We wanted to examine the effect of adding a third oral agent, faropenem, to this dual combination. Methods. We performed a series of studies in the hollow fiber system model of intracellular Mycobacterium tuberculosis, by mimicking pediatric pharmacokinetics of each antibiotic. First, we varied the percentage of time that faropenem persisted above minimum inhibitory concentration (TMIC) on the moxifloxacin-linezolid regimen. After choosing the best faropenem exposure, we performed experiments in which we varied the moxifloxacin and linezolid doses in the triple regimen. Finally, we performed longer-duration therapy validation experiments. Bacterial burden was quantified using both colony-forming units per milliliter (CFU/mL) and time to positivity (TTP). Kill slopes were modeled using exponential regression. Results. TTP was a more sensitive measure of bacterial burden than CFU/mL. A faropenem TMIC > 62% was associated with steepest microbial kill slope. Regimens of standard linezolid and moxifloxacin plus faropenem TMIC > 60%, as well as higher-dose moxifloxacin, achieved slopes equivalent to those of the standard regimen based by both TTP and CFU/mL over 28 days of treatment. Conclusions. We have developed an oral faropenem-linezolid-moxifloxacin (FLAME) regimen that is free of first-line drugs. The regimen could be effective against both multidrug-resistant and drug-susceptible tuberculosis in children. PMID:27742640

  18. Activity of and Resistance to Moxifloxacin in Staphylococcus aureus

    OpenAIRE

    Ince, Dilek; Zhang, Xiamei; Hooper, David C.

    2003-01-01

    Moxifloxacin has enhanced potency against Staphylococcus aureus, lower propensity to select for resistant mutants, and higher bactericidal activity against highly resistant strains than ciprofloxacin. Despite similar activity against purified S. aureus topoisomerase IV and DNA gyrase, it selects for topoisomerase IV mutants, making topoisomerase IV the preferred target in vivo.

  19. Moxifloxacin dosing in post-bariatric surgery patients

    NARCIS (Netherlands)

    Colin, Pieter; Eleveld, Douglas J.; Struys, Michel M. R. F.; T'Jollyn, Huybrecht; Van Bortel, Luc M.; Ruige, Johannes; De Waele, Jan; Van Bocxlaer, Jan; Boussery, Koen

    2014-01-01

    Introduction Given the ever increasing number of obese patients and obesity related bypass surgery, dosing recommendations in the post-bypass population are needed. Using a population pharmacokinetic (PK) analysis and PK-pharmacodynamic (PD) simulations, we investigated whether adequate moxifloxacin

  20. Rifampicin reduces plasma concentrations of moxifloxacin in patients with tuberculosis.

    NARCIS (Netherlands)

    Nijland, H.M.J.; Ruslami, R.; Suroto, A.J.; Burger, D.M.; Alisjahbana, B.; Crevel, R. van; Aarnoutse, R.E.

    2007-01-01

    BACKGROUND: The long duration of the current tuberculosis (TB) treatment is demanding and warrants the development of new drugs. Moxifloxacin shows promising results and may be combined with rifampicin to shorten the duration of TB treatment. Rifampicin induces the phase II metabolic enzymes that ar

  1. Safety of Moxifloxacin following repeated intramuscular administration in Wistar rats

    Directory of Open Access Journals (Sweden)

    K.A. Sadariya

    Full Text Available Moxifloxacin is a novel fourth generation fluoroquinolone with broad spectrum of antibacterial activity. The study was conducted to evaluate the safety of Moxifloxacin (5.0 mg/kg after repeated intramuscular administration at 24 h interval for 14 days in male and female wistar rats. Hematological (Haemoglobin, RBC, WBC, MCV, MCH, MCHC, HCT and DLC, blood biochemical parameters (AST, ALT, ALP, Total Bilirubin, Total Serum Protein, Serum Albumin, Globulin, Serum Creatinine, Urea, Uric acid and Blood glucose and histopathological examination of various tissues were carried out in the present study. Male and female animals of any group did not reveal any clinical symptoms and mortality attributable to the 14 days intramuscular administration of Moxifloxacin. The data were compared by unpaired two tail `t` test using Graph Pad Prism (Version 4.00. All above hematological and blood biochemical parameters were found to fluctuate within normal range during treatment period and the mean values were not significantly differ (p < 0.05 from corresponding control values. Moreover, no gross or microscopic changes were found in the liver, kidney, heart, spleen, stomach, intestine and joint cartilages of the treated wistar rats. Results indicate that daily administration of Moxifloxacin for 14 days seems to be safe and well tolerated in rats. [Veterinary World 2010; 3(10.000: 449-452

  2. DEVELOPMENT OF COMPOSITION OF INFUSION MEDICATION ON BASIS OF MOXIFLOXACIN

    Directory of Open Access Journals (Sweden)

    Almakaeva L.G.

    2015-05-01

    Full Text Available Application of fluoroquinolones covers by experience of treatment more than 800 million patients, and presently they are one of basic classes in the antimicrobial arsenal of practical medicine. Such achievements became possible after the clear understanding of intercommunication of structure and activity of molecules of this class of antibiotics. This knowledge became the basis for the synthesis of new derivatives with a wide range, powerful activity and improved pharmacokinetic profile for the best clinical outcome. Moxifloxacin is 8-methoxyfluoroquinolon of wide spectrum which interacts mainly with DNA gyrase of gram-negative and with topoisomerase of IV type of gram-positive bacteria. He has the extended activity against gram-positive cocci, however keeps activity against gram-negative bacteria. Moxifloxacin also has good activity against atypical respiratory pathogens (Legionella of pneumophila, Chlamydia of pneumoniae and Mycoplasma of pneumoniae. Another his feature is high anti-anaerobic activity. Therefore development of domestic medication with Moxifloxacin - a fluoroquinolone 4 generations - is actual. Materials and methods Research material was a substance of Moxifloxacin hydrochloride, produced by firm «Sansh Biotech Pvt. Ltd.», India, a dosage form on the basis of Moxifloxacin - solution for infusion. Qqualitative and quantitative control of samples of the drug were conducted on parameters which characterize stability: рН, content of active substance, transparency, colour, related impurities, mechanical inclusions on methods, which are described in SPhU. Results and Discussion Proposed the drug is antibiotic of wide spectrum of action of fluoroquinolone. Moxifloxacin hydrochloride is powder pale yellow with slightly hygroscopic nature. He moderately dissolve in water and methanol, poorly will dissolve in hydrochloric acid and ethanol, and practically will not dissolve in an acetone and toluene. рН 0,2 % solution is in a range

  3. Streptococcus pneumoniae: sensibilidade a penicilina e moxifloxacina Streptococcus pneumoniae: susceptibility to penicillin and moxifloxacin

    Directory of Open Access Journals (Sweden)

    Flávia Rossi

    2012-02-01

    Full Text Available OBJETIVO: Determinar a concentração inibitória mínima (CIM de penicilina parenteral e moxifloxacina contra cepas de Streptococcus pneumoniae isoladas em um centro hospitalar. Métodos: Estudo in vitro prospectivo de 100 isolados de S. pneumoniae coletados de pacientes tratados entre outubro de 2008 e julho de 2010 no complexo do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, em São Paulo (SP. Os isolados foram obtidos de culturas do trato respiratório e de amostras de sangue não relacionadas a infecções meníngeas e foram testados quanto à suscetibilidade a penicilina e moxifloxacina por E test. As interpretações categóricas de CIM foram baseadas em padrões atualizados. RESULTADOS: Todos os isolados foram suscetíveis a penicilina parenteral (CIM OBJECTIVE: To determine the minimum inhibitory concentrations (MICs of parenteral penicillin and moxifloxacin against Streptococcus pneumoniae strains isolated at a hospital center. METHODS: In-vitro, prospective study involving 100 S. pneumoniae isolates collected from patients who had been treated, between October of 2008 and July of 2010, at the Hospital das Clínicas complex of the University of São Paulo School of Medicine, located in the city of São Paulo, Brazil. The isolates were obtained from respiratory tract cultures or blood samples unrelated to meningeal infections, and they were tested for penicillin and moxifloxacin susceptibility by E-test. The MIC category interpretations were based on updated standards. RESULTS: All isolates were fully susceptible to parenteral penicillin (MIC < 2 µg/mL, and, consequently, they were also susceptible to amoxicillin, ampicillin, third/fourth generation cephalosporins, and ertapenem. Of the S. pneumoniae strains, 99% were also susceptible to moxifloxacin, and only one strain showed an MIC = 1.5 µg/mL (intermediate. CONCLUSIONS: Our results showed high susceptibility rates to parenteral penicillin and

  4. The Effects of Moxifloxacin on QTc Interval in Healthy Korean Male Subjects

    OpenAIRE

    Moon, Seol Ju; Lee, Jongtae; An, Hyungmi; Yim, Dong-Seok; Chung, Jae-Yong; Yu, Kyung-Sang; Cho, Joo-Youn; Lim, Kyoung Soo

    2014-01-01

    Background and objective Moxifloxacin 400 mg is a widely used positive control in thorough QT (TQT) studies, but its QT-prolonging effects in Korean subjects have not been studied. The present study was conducted to collect pilot data in Korean subjects after moxifloxacin administration to evaluate the adequacy of moxifloxacin as a positive control. Methods Thirty-eight, healthy, Korean, male subjects were recruited for pharmacokinetic (PK) blood sampling and electrocardiography (ECG) recordi...

  5. Simultaneous determination of moxifloxacin and cefixime by first and ratio first derivative ultraviolet spectrophotometry

    OpenAIRE

    Attimarad Mahesh; Al-Dhubiab Bander E; Alhaider Ibrahim A; Nair Anroop B; N Sree; K Mueen

    2012-01-01

    Abstract Background The new combination of moxifloxacin HCl and cefixime trihydrate is approved for the treatment of lower respiratory tract infections in adults. At initial formulation development and screening stage a fast and reliable method for the dissolution and release testing of moxifloxacin and cefixime were highly desirable. The zero order overlaid UV spectra of moxifloxacin and cefixime showed >90% overlapping. Hence, simple, accurate precise and validated two derivative spectropho...

  6. In Vitro Postantibiotic Effects of Rifapentine, Isoniazid, and Moxifloxacin against Mycobacterium tuberculosis

    OpenAIRE

    Chan, Chiu-Yeung; Au-Yeang, Carrie; Yew, Wing-Wai; Leung, Chi-Chiu; Cheng, Augustine F. B.

    2004-01-01

    Postantibiotic effects (PAEs) of rifapentine, isoniazid, and moxifloxacin against Mycobacterium tuberculosis ATCC 27294 were studied using a radiometric culture system. Rifapentine at 20 mg/liter gave the longest PAE (104 h) among the drugs used alone. The combinations of rifapentine plus isoniazid, rifapentine plus moxifloxacin, and isoniazid plus moxifloxacin gave PAEs of 136.5, 59.0, and 8.3 h, respectively.

  7. Early Bactericidal Activity of Moxifloxacin in Treatment of Pulmonary Tuberculosis: a Prospective, Randomized Study

    OpenAIRE

    Pletz, Mathias W. R.; De Roux, Andres; Roth, Andreas; NEUMANN, Karl-Heinz; Mauch, Harald; Lode, Hartmut

    2004-01-01

    Moxifloxacin is the most active fluoroquinolone against Mycobacterium tuberculosis in vitro. However, data about the efficacy in patients are not available. We enrolled 17 patients with tuberculosis in a prospective, randomized study. After 5 days of monotherapy with either moxifloxacin or isoniazid, we detected significant decreases in mean CFU per milliliter in sputum in both groups. The calculated early bactericidal activities for isoniazid and moxifloxacin were 0.209 and 0.273 log10 CFU p...

  8. Review of moxifloxacin hydrochloride ophthalmic solution in the treatment of bacterial eye infections

    OpenAIRE

    Darlene Miller

    2008-01-01

    Darlene MillerAbrams Ocular Microbiology Laboratory, Bascom Palmer Eye Institute, Anne Bates Leach Eye Hospital, Miller School of Medicine-University of Miami, FL, USAAbstract: Moxifloxacin hydrochloride ophthalmic solution 0.5% (Vigamox®) is the ocular formulation/adaptation of moxifloxacin. Moxifloxacin is a broad spectrum 8-methoxyfluoroquinolone which terminates bacterial growth by binding to DNA gyrase (topoisomerase II) and topoisomerase IV, essential bacterial enzymes involved ...

  9. Comparison of the In Vitro Efficacies of Moxifloxacin and Amoxicillin against Listeria monocytogenes▿ †

    OpenAIRE

    Grayo, S.; Join-Lambert, O.; Desroches, M. C.; Le Monnier, A.

    2008-01-01

    Listeria monocytogenes is a facultative intracellular bacterium that causes severe infections associated with a high mortality rate. Moxifloxacin presents extended activity against gram-positive bacteria and has recently been suggested to be a potential alternative in the treatment of listeriosis. We evaluated the in vitro efficacy of moxifloxacin against L. monocytogenes using a combination of epidemiological and experimental approaches. The median MIC of moxifloxacin for a large collection ...

  10. In Vitro Activities of Moxifloxacin and Other Fluoroquinolones against Mycoplasma pneumoniae

    OpenAIRE

    Hamamoto, Kumiko; Shimizu, Takashi; Fujimoto, Naoyuki; Zhang, Ye; Arai, Sumio

    2001-01-01

    A total of 105 isolates of Mycoplasma pneumoniae were evaluated for susceptibility to moxifloxacin, sparfloxacin, levofloxacin, and ciprofloxacin. Moxifloxacin, a newly synthesized compound, showed the greatest activity. The MICs and MBCs at which 50 and 90% of isolates were affected were 0.15 (MIC50 and MBC50) and 0.3 μg/ml (MIC90 and MBC90) respectively. The results indicate that moxifloxacin might be promising an antimycoplasmal agent.

  11. Moxifloxacin Retains Antimycobacterial Activity in the Presence of gyrA Mutations

    OpenAIRE

    McGrath, Marieta; Gey van Pittius, Nico C.; Sirgel, Frederick A.; van Helden, Paul D.; Warren, Robin M

    2014-01-01

    Moxifloxacin-resistant Mycobacterium tuberculosis mutants were selected in vitro using different concentrations of moxifloxacin. gyrA mutations at codons 88 and 94 were associated with resistance (defined as an MIC of ≥2 μg/ml) (P < 0.0001 and P = 0.0053, respectively). Despite the presence of gyrA mutations, moxifloxacin significantly impedes bacterial growth, supporting its use for the treatment of ofloxacin-resistant M. tuberculosis.

  12. A protocol for conducting 7-day daily renewal tests with Lemna gibba.

    Science.gov (United States)

    Brain, Richard A; Solomon, Keith R

    2007-01-01

    Lemna gibba (a duckweed) is a freshwater macrophyte commonly used in toxicity testing, and Lemna spp are currently the only aquatic higher plants required for evaluation of pesticides under the pesticide registration guidelines of the EPA. The methods currently available for toxicity testing by various organizations and agencies, including ASTM, OECD, EPA and Environment Canada, are largely static or semistatic tests with unspecified renewal intervals (OECD) and may not provide a consistent means of exposure owing to short toxicant half-life in aquatic media, uptake of chemical by plants and evaporation of nutrient media. The procedure outlined here details a simple and efficient 7-day daily static renewal procedure for conducting toxicity tests with L. gibba, the appropriate end points to assess, the statistical criteria necessary for analyzing the toxicity data, as well as the steps required to culture and maintain L. gibba. This protocol is based on a modified version of a widely accepted static method. PMID:17446897

  13. Impairment of IFN-gamma response to synthetic peptides of Mycobacterium tuberculosis in a 7-day whole blood assay.

    Directory of Open Access Journals (Sweden)

    Hannah Priyadarshini Gideon

    Full Text Available Studies on Mycobacterium tuberculosis (MTB antigens are of interest in order to improve vaccine efficacy and to define biomarkers for diagnosis and treatment monitoring. The methodologies used for these investigations differ greatly between laboratories and discordant results are common. The IFN-gamma response to two well characterized MTB antigens ESAT-6 and CFP-10, in the form of recombinant proteins and synthetic peptides, was evaluated in HIV-1 uninfected persons in both long-term (7 day and 24 hour, commercially available QuantiFERON TB Gold in Tube (QFT-GIT, whole blood assays. Our findings showed differences in the IFN-gamma response between 24 hour and 7 day cultures, with recombinant proteins inducing a significantly higher response than the peptide pools in 7 day whole blood assays. The activity of peptides and recombinant proteins did not differ in 24 hour whole blood or peripheral blood mononuclear cell (PBMC based assays, nor in the ELISpot assay. Further analysis by SELDI-TOF mass spectrometry showed that the peptides are degraded over the course of 7 days of incubation in whole blood whilst the recombinant proteins remain intact. This study therefore demonstrates that screening antigenic candidates as synthetic peptides in long-term whole blood assays may underestimate immunogenicity.

  14. Structural basis of DNA gyrase inhibition by antibacterial QPT-1, anticancer drug etoposide and moxifloxacin.

    Science.gov (United States)

    Chan, Pan F; Srikannathasan, Velupillai; Huang, Jianzhong; Cui, Haifeng; Fosberry, Andrew P; Gu, Minghua; Hann, Michael M; Hibbs, Martin; Homes, Paul; Ingraham, Karen; Pizzollo, Jason; Shen, Carol; Shillings, Anthony J; Spitzfaden, Claus E; Tanner, Robert; Theobald, Andrew J; Stavenger, Robert A; Bax, Benjamin D; Gwynn, Michael N

    2015-01-01

    New antibacterials are needed to tackle antibiotic-resistant bacteria. Type IIA topoisomerases (topo2As), the targets of fluoroquinolones, regulate DNA topology by creating transient double-strand DNA breaks. Here we report the first co-crystal structures of the antibacterial QPT-1 and the anticancer drug etoposide with Staphylococcus aureus DNA gyrase, showing binding at the same sites in the cleaved DNA as the fluoroquinolone moxifloxacin. Unlike moxifloxacin, QPT-1 and etoposide interact with conserved GyrB TOPRIM residues rationalizing why QPT-1 can overcome fluoroquinolone resistance. Our data show etoposide's antibacterial activity is due to DNA gyrase inhibition and suggests other anticancer agents act similarly. Analysis of multiple DNA gyrase co-crystal structures, including asymmetric cleavage complexes, led to a 'pair of swing-doors' hypothesis in which the movement of one DNA segment regulates cleavage and religation of the second DNA duplex. This mechanism can explain QPT-1's bacterial specificity. Structure-based strategies for developing topo2A antibacterials are suggested. PMID:26640131

  15. Synthesis, characterization and antimicrobial investigation of some moxifloxacin metal complexes

    Science.gov (United States)

    Sadeek, Sadeek A.; El-Shwiniy, Walaa H.; El-Attar, Mohamed S.

    2011-12-01

    The new complexes of moxifloxacin (MOX), with Ti(IV), Y(III), Pd(II) and Ce(IV) have been synthesized. These complexes were then characterized by melting point, magnetic studies and spectroscopic techniques involving infrared spectra (IR), UV-Vis, 1H NMR. C, H, N and halogen elemental analysis and thermal behavior of complexes also investigated. The results suggested that the molar ratio for all complexes is M: MOX = 1:2 where moxifloxacin acts as a bidentate via one of the oxygen atoms of the carboxylate group and through the ring carbonyl group and the complexes have the following formula [Ti(MOX) 2](SO 4) 2·7H 2O, [Y(MOX) 2Cl 2]Cl·12H 2O, [Pd(MOX) 2(H 2O) 2]Cl 2·6H 2O and [Ce(MOX) 2](SO 4) 2·2H 2O. The activation energies, E*, enthalpies, Δ H*, entropies, Δ S* and Gibbs free energies, Δ G*, of the thermal decomposition reactions have been derived from thermogravimetric (TGA) and differential thermogravimetric (DrTG) curves, using Coats-Redfern (CR) and Horowitz-Metzger (HM) methods. The antimicrobial activity of these complexes has been evaluated against three Gram-positive and three Gram-negative bacteria and compared with the reference drug moxifloxacin. The antibacterial activity of Ti(IV) complex is significant for E. coli K32 and highly significant for S. aureus K1, B. subtilis K22, Br. otitidis K76, P. aeruginosa SW1 and K. oxytoca K42 compared with free moxifloxacin.

  16. Evaluation of Moxifloxacin, a New 8-Methoxyquinolone, for Treatment of Meningitis Caused by a PenicillinResistant Pneumococcus in Rabbits

    OpenAIRE

    Østergaard, Christian; Klitmøller Sørensen, Tina; Dahl Knudsen, Jenny; Frimodt-Møller, Niels

    1998-01-01

    Moxifloxacin is a new 8-methoxyquinolone with high activity against gram-positive bacteria, including penicillin-resistant pneumococci. In an experimental meningitis model, we studied the pharmacokinetics of moxifloxacin in infected and uninfected rabbits and evaluated the antibiotic efficacies of moxifloxacin, ceftriaxone, and vancomycin against a penicillin-resistant Streptococcus pneumoniae strain (penicillin, ceftriaxone, vancomycin, and moxifloxacin MICs were 1, 0.5, 0.5, and 0.125 μg/ml...

  17. Susceptibilities of Campylobacter jejuni Isolates from Germany to Ciprofloxacin, Moxifloxacin, Erythromycin, Clindamycin, and Tetracycline

    OpenAIRE

    Wagner, Jutta; Jabbusch, Miriam; Eisenblätter, Martin; Hahn, Helmut; Wendt, Constanze; Ignatius, Ralf

    2003-01-01

    To elucidate Campylobacter jejuni resistance to antibiotics in Germany, MICs of ciprofloxacin, moxifloxacin, erythromycin, clindamycin, and tetracycline were determined (using agar dilution) for 144 clinical isolates. The data indicate a considerable ciprofloxacin resistance (45.1%) without a clonal relationship of the strains and a greater in vitro activity of moxifloxacin, erythromycin, and clindamycin.

  18. Selection of Streptococcus pneumoniae Mutants Having Reduced Susceptibility to Moxifloxacin and Levofloxacin

    OpenAIRE

    Li, Xinying; Zhao, Xilin; Drlica, Karl

    2002-01-01

    With Streptococcus pneumoniae, moxifloxacin was 4- and 10-fold more effective than levofloxacin at restricting selection of resistant mutants and at killing resistant mutants, respectively. The selection frequency for first-step topoisomerase mutants was 1,000 times lower for moxifloxacin than for levofloxacin; this difference was lost when second-step mutants were selected.

  19. Moxifloxacin Lethality against Mycobacterium tuberculosis in the Presence and Absence of Chloramphenicol

    OpenAIRE

    Malik, Muhammad; Drlica, Karl

    2006-01-01

    The C-8-methoxy fluoroquinolone moxifloxacin was more lethal against chloramphenicol-treated Mycobacterium tuberculosis than Bay y3114, a C-8-H cognate of moxifloxacin, and two C-8-methoxy fluoroquinolones, gatifloxacin and BMS-433368, which have different C-7 substituents. Thus, an optimal combination of C-7 and C-8 substituents is likely to be important for killing nongrowing M. tuberculosis.

  20. Effects of Subinhibitory Concentrations of Antibiotics on Colonization Factor Expression by Moxifloxacin-Susceptible and Moxifloxacin-Resistant Clostridium difficile Strains▿

    OpenAIRE

    Denève, Cécile; Bouttier, Sylvie; Dupuy, Bruno; Barbut, Frédéric; Collignon, Anne; Janoir, Claire

    2009-01-01

    Recent outbreaks of Clostridium difficile infection have been related to the emergence of the NAP1/027 epidemic strain. This strain demonstrates increased virulence and resistance to the C-8-methoxyfluoroquinolones gatifloxacin and moxifloxacin. These antibiotics have been implicated as major C. difficile infection-inducing agents. We investigated by real-time reverse transcription-PCR the impact of subinhibitory concentrations of ampicillin, clindamycin, ofloxacin, and moxifloxacin on the ex...

  1. Simultaneous determination of moxifloxacin and cefixime by first and ratio first derivative ultraviolet spectrophotometry

    Directory of Open Access Journals (Sweden)

    Attimarad Mahesh

    2012-09-01

    Full Text Available Abstract Background The new combination of moxifloxacin HCl and cefixime trihydrate is approved for the treatment of lower respiratory tract infections in adults. At initial formulation development and screening stage a fast and reliable method for the dissolution and release testing of moxifloxacin and cefixime were highly desirable. The zero order overlaid UV spectra of moxifloxacin and cefixime showed >90% overlapping. Hence, simple, accurate precise and validated two derivative spectrophotometric methods have been developed for the determination of moxifloxacin and cefixime. Methods In the first derivative spectrophotometric method varying concentration of moxifloxacin and cefixime were prepared and scanned in the range of 200 to 400 nm and first derivative spectra were calculated (n = 1. The zero crossing wavelengths 287 nm and 317.9 nm were selected for determination of moxifloxacin and cefixime, respectively. In the second method the first derivative of ratio spectra was calculated and used for the determination of moxifloxacin and cefixime by measuring the peak intensity at 359.3 nm and 269.6 nm respectively. Results Calibration graphs were established in the range of 1–16 μg /mL and 1–15 μg /mL for both the drugs by first and ratio first derivative spectroscopic methods respectively with good correlation coefficients. Average accuracy of assay of moxifloxacin and cefixime were found to be 100.68% and 98 93%, respectively. Relative standard deviations of both inter and intraday assays were less than 1.8%. Moreover, recovery of moxifloxacin and cefixime was more than 98.7% and 99.1%, respectively. Conclusions The described derivative spectrophotometric methods are simple, rapid, accurate, precise and excellent alternative to sophisticated chromatographic techniques. Hence, the proposed methods can be used for the quality control of the cited drugs and can be extended for routine analysis of the drugs in formulations.

  2. Uptake and Intracellular Activity of Moxifloxacin in Human Neutrophils and Tissue-Cultured Epithelial Cells

    OpenAIRE

    Pascual, Alvaro; García, Isabel; Ballesta, Sofía; Perea, Evelio J.

    1999-01-01

    The penetration by moxifloxacin of human neutrophils (polymorphonuclear leukocytes [PMN]) and tissue-cultured epithelial cells (McCoy cells) was evaluated by a fluorometric assay. At extracellular concentrations of 5 mg/liter, the cellular-to-extracellular concentration ratios (C/E) of moxifloxacin in PMN and McCoy cells were 10.9 ± 1.0 and 8.7 ± 1.0, respectively (20 min; 37°C). The uptake of moxifloxacin by PMN was rapid, reversible, nonsaturable (at extracellular concentrations ranging fro...

  3. Orally administered moxifloxacin prolongs QTc in healthy Chinese volunteers: a randomized, single-blind, crossover study

    OpenAIRE

    Chen, Qian; Liu, Yan-Mei; Liu, Yun; Mendzelevski, Boaz; Chanter, Dennis; Pu, Hua-hua; Liu, Gang-yi; Weng, Onglee; Hu, Chao-Ying; Wang, Wei; Yu, Chen; Jia, Jing-Ying

    2015-01-01

    Aim: To investigate the QT/QTc effects of orally administered moxifloxacin in healthy Chinese volunteers. Methods: This was a single-blinded, randomized, single-dose, placebo-controlled, two-period cross-over study. A total of 24 healthy Chinese volunteers were enrolled, randomly assigned to two groups: one group received moxifloxacin (400 mg, po) followed by placebo with a 7-d interval, another group received placebo followed by moxifloxacin with a 7-d interval. On the days of dosing, 12-lea...

  4. Exercise-induced pyruvate dehydrogenase activation is not affected by 7 days of bed rest

    DEFF Research Database (Denmark)

    Kiilerich, Kristian; Jørgensen, Stine Ringholm; Biensø, Rasmus Sjørup;

    2011-01-01

    To test the hypothesis that physical inactivity impairs the exercise-induced modulation of pyruvate dehydrogenase (PDH), 6 healthy normally physically active male subjects completed 7 days of bed rest. Before and immediately after the bed rest, the subjects completed an OGTT and a one-legged knee...

  5. A Case Report of Severe Corneal Toxicity following 0.5% Topical Moxifloxacin Use

    Directory of Open Access Journals (Sweden)

    A.P. Vignesh

    2015-02-01

    Full Text Available Moxifloxacin is a widely used topical antibiotic in various bacterial infections of the eye. Its safety and efficacy have been proved by many studies. We report a case of a rare adverse effect following its use. A 10-year-old female who had presented with acute bacterial conjunctivitis in both eyes with no corneal involvement was started on preservative-free 0.5% topical moxifloxacin four times a day. The child developed a severe form of corneal toxicity in both eyes with circumcorneal congestion and corneal edema following its use. The child's visual acuity had dropped from 20/20 to 20/400 in both the eyes. Topical moxifloxacin was discontinued, following which the cornea cleared dramatically and the visual acuity became normal. This case indicates that though rare, topical moxifloxacin can cause severe keratitis and that more studies need to be conducted to evaluate its safety.

  6. Review of moxifloxacin hydrochloride ophthalmic solution in the treatment of bacterial eye infections

    Directory of Open Access Journals (Sweden)

    Darlene Miller

    2008-03-01

    Full Text Available Darlene MillerAbrams Ocular Microbiology Laboratory, Bascom Palmer Eye Institute, Anne Bates Leach Eye Hospital, Miller School of Medicine-University of Miami, FL, USAAbstract: Moxifloxacin hydrochloride ophthalmic solution 0.5% (Vigamox® is the ocular formulation/adaptation of moxifloxacin. Moxifloxacin is a broad spectrum 8-methoxyfluoroquinolone which terminates bacterial growth by binding to DNA gyrase (topoisomerase II and topoisomerase IV, essential bacterial enzymes involved in the replication, translation, repair and recombination of deoxyribonucleic acid. Affinity for both enzymes improves potency and reduces the probability of selecting resistant bacterial subpopulations. Vigamox is a bactericidal, concentration dependent, anti-infective. It is preservative free, and well tolerated with minimal ocular side effects. It provides increased penetration into ocular tissues and fluids with improved activity against Streptococci and Staphylococci species and moderate to excellent activity against clinically relevant, gram- negative ocular pathogens.Keywords: moxifloxacin, vigamox, pharmacodynamic indices, minimal inhibitory concentrations

  7. Toxicity and bioconcentration of the pharmaceuticals moxifloxacin, rosuvastatin, and drospirenone to the unionid mussel Lampsilis siliquoidea.

    Science.gov (United States)

    Gilroy, Eve A M; Klinck, Joel S; Campbell, Sheena D; McInnis, Rodney; Gillis, Patricia L; de Solla, Shane R

    2014-07-15

    Pharmaceuticals and personal care products (PPCPs) and their metabolites are continually released from wastewater treatment plants into the aquatic environment; however, their impact on aquatic biota is poorly understood. This study examined the toxicity and bioconcentration of three pharmaceuticals: moxifloxacin, rosuvastatin, and drospirenone to the unionid mussel Lampsilis siliquoidea. Effects of moxifloxacin and rosuvastatin were assessed through aqueous 21-d static-renewal tests using 2-year-old mussels, at 0.01, 0.1, 1, 10 and 100mg/L (nominal concentrations). Following exposure, survival, behavior, algal clearance rate, hemocyte viability and density, and glutathione S-transferase (GST) activity were assessed. In addition, the acute (48 h) toxicity of moxifloxacin (0-100mg/L) and drospirenone (0-3mg/L) to glochidia (larval mussels) were examined. In 21 day exposures (2-yr old mussels), there were no differences in survival, oxygen consumption, hemocyte density, or GST activity over the range of concentrations examined; however, the proportion of time mussels spent filtering, and consequently the algal clearance rate, decreased at the higher moxifloxacin and rosuvastatin concentrations. Bioconcentration factors (BCFs) ranged between 0.03 and 70 for moxifloxacin, and between 0 and 0.05 for rosuvastatin for exposures up to 100mg/L. The BCF for moxifloxacin at the highest exposure concentration was lower than that at the mid-level concentrations, likely due to decreased filtering activity at the higher exposure levels. The feeding rates declined and the amount of time the subadult mussels spent with their valves closed increased at the higher moxifloxacin and rosuvastatin exposures. Glochidia viability did not vary with exposure to drospirenone, but declined at the highest moxifloxacin concentration, resulting in an EC50 of 120 mg/L. Overall, observed sublethal and lethal effects occurred at concentrations which exceed expected environmental concentrations

  8. Penetration of Moxifloxacin into Healthy and Inflamed Subcutaneous Adipose Tissues in Humans

    OpenAIRE

    Joukhadar, Christian; Stass, Heino; Müller-Zellenberg, Ulrike; Lackner, Edith; Kovar, Florian; Minar, Erich; Müller, Markus

    2003-01-01

    The present study addressed the ability of moxifloxacin to penetrate into healthy and inflamed subcutaneous adipose tissues in 12 patients with soft tissue infections (STIs). Penetration of moxifloxacin into the interstitial space fluid of healthy and inflamed subcutaneous adipose tissues was measured by use of in vivo microdialysis following administration of a single intravenous dosage of 400 mg in six diabetic and six nondiabetic patients with STIs. For the entire study population, the mea...

  9. Rapid Eradication of Listeria monocytogenes by Moxifloxacin in a Murine Model of Central Nervous System Listeriosis▿

    OpenAIRE

    Grayo, Solène; Lott-Desroches, Marie-Catherine; Dussurget, Olivier; Respaud, Renaud; Fontanet, Arnaud; Join-Lambert, Olivier; Singlas, Eric; Le Monnier, Alban

    2008-01-01

    Listeriosis is a rare but life-threatening infection. A favorable outcome is greatly aided by early administration of antibiotics with rapid bactericidal activity against Listeria monocytogenes. Moxifloxacin, a new-generation fluoroquinolone with extended activity against gram-positive bacteria, has proved its effectiveness in vitro against intracellular reservoirs of bacteria. The efficacies of moxifloxacin and amoxicillin were compared in vivo by survival curve assays and by studying the ki...

  10. Moxifloxacin induced fatal hepatotoxicity in a 72-year-old man: a case report

    OpenAIRE

    Verma, Rajanshu; Dhamija, Radhika; Batts, Donald H.; Stephen C Ross; Loehrke, Mark E

    2009-01-01

    Moxifloxacin is a newer-generation synthetic fluoroquinolone that is used for treatment of acute bacterial sinusitis, acute exacerbation of chronic bronchitis, community acquired pneumonia, intra-abdominal infections and skin/skin structure infections. We describe a case of fatal hepatotoxicity caused by Moxifloxacin in a 72-year-old man. He presented with jaundice and epigastric tenderness that started one week after being treated for acute exacerbation of his chronic bronchitis with Moxiflo...

  11. Effects of Moxifloxacin on Human Neutrophil and T-Lymphocyte Functions in Vitro

    OpenAIRE

    Ronald Anderson; Riana Cockeran; Charles Feldman; Theron, Annette J.; Moliehi Potjo

    2010-01-01

    Moxifloxacin is useful in the treatment of respiratory infections, including community-acquired pneumonia, and also shows promise in the treatment of tuberculosis, a clinical setting which necessitates extended administration of this agent. Relatively little is known, however, about the effects of this agent on the antimicrobial and proliferative activities of human neutrophils and T-lymphocytes, respectively. In the current study, we have investigated the effects of moxifloxacin at therapeut...

  12. Potentiometric determination of moxifloxacin in some pharmaceutical formulation using PVC membrane sensors

    OpenAIRE

    Hefnawy, Mohammed M; Homoda, Atef M; Abounassif, Mohammed A; Alanazi, Amer M; Al-Majed, Abdulrahaman; Mostafa, Gamal A

    2014-01-01

    Background The construction and electrochemical response characteristics of Poly (vinyl chloride) membrane sensors for moxifloxacin HCl (MOX) are described. The sensing membranes incorporate ion association complexes of moxifloxacin cation and sodium tetraphenyl borate (NaTPB) (sensor 1), phosphomolybdic acid (PMA) (sensor 2) or phosphotungstic acid (PTA) (sensor 3) as electroactive materials. Results The sensors display a fast, stable and near-Nernstian response over a relative wide moxiflox...

  13. The influence of continuous venovenous haemodialysis on the pharmacokinetics of multiple oral moxifloxacin administration to patients with severe renal dysfunction

    OpenAIRE

    Stass, H; Bührmann, S; Mitchell, A.; Kubitza, D; Möller, J-G; Kribben, A; Wenzel, R R; Schäfers, R F

    2007-01-01

    What is already known about this subjectAlthough renal excretion is one important route of excretion of moxifloxacin and its metabolites, moxifloxacin pharmacokinetics are similar in patients with varying degrees of renal impairment (but not on dialysis) and in healthy subjects.What this study addsThis study showed that moxifloxacin pharmacokinetics are comparable in patients with severe renal failure requiring haemodialysis and in healthy subjects and patients with impaired renal function no...

  14. Huge congenital cervical immature teratoma mimicking lymphatic malformation in a 7-day-old male neonate

    Directory of Open Access Journals (Sweden)

    Jihoon Jang

    2016-05-01

    Full Text Available Most cervical teratomas are benign. However, because of their location and size, these neoplasms cause airway obstruction, and the prognosis of patients is poor if left untreated. Early diagnosis by antenatal ultrasound, airway management, and a multidisciplinary team approach to treatment are therefore necessary. This report describes a 7-day-old male neonate who presented at our institution with a huge neck mass that was treated by surgical excision.

  15. In vitro function of random donor platelets stored for 7 days in composol platelet additive solution

    Directory of Open Access Journals (Sweden)

    Gupta Ashish

    2011-01-01

    Full Text Available Background and Aim: Platelets are routinely isolated from whole blood and stored in plasma for 5 days. The present study was done to assess the in vitro function of random donor platelets stored for 7 days in composol platelet additive solution at 22°C. Materials and Methods: The study sample included 30 blood donors of both sex in State Blood Bank, CSM Medical University, Lucknow. Random donor platelets were prepared by platelet rich plasma method. Whole blood (350 ml was collected in anticoagulant Citrate Phosphate Dextrose Adenine triple blood bags. Random donor platelets were stored for 7 days at 22°C in platelet incubators and agitators, with and without additive solution. Results: Platelet swirling was present in all the units at 22°C on day 7, with no evidence of bacterial contamination. Comparison of the mean values of platelet count, platelet factor 3, lactate dehydrogenase, pH, glucose and platelet aggregation showed no significant difference in additive solution, whereas platelet factor 3, glucose and platelet aggregation showed significant difference (P < 0.001 on day 7 without additive solution at 22°C. Conclusion: Our study infers that platelet viability and aggregation were best maintained within normal levels on day 7 of storage in platelet additive solution at 22°C. Thus, we may conclude that in vitro storage of random donor platelets with an extended shelf life of 7 days using platelet additive solution may be advocated to improve the inventory of platelets.

  16. Moxifloxacin (BAY12-8039), a New 8-Methoxyquinolone, Is Active in a Mouse Model of Tuberculosis

    OpenAIRE

    Miyazaki, Eishi; Miyazaki, Miki; Chen, Jong Min; Chaisson, Richard E.; Bishai, William R.

    1999-01-01

    Moxifloxacin (BAY12-8039) is a new 8-methoxyquinolone shown to be active against Mycobacterium tuberculosis in vitro. We tested moxifloxacin for activity in mice against M. tuberculosis CSU93, a highly virulent, recently isolated clinical strain. The MIC of moxifloxacin for the CSU93 strain was 0.25 μg/ml. The serum moxifloxacin concentration after oral administration in mice peaked within 0.25 h, reaching 7.8 μg/ml with doses of 100 mg/kg of body weight; the maximum concentration and the ana...

  17. 莫西沙星与左氧氟沙星治疗社区获得性肺炎的临床研究%The Clinical stady of moxifloxacin and levofloxacin in the treatment of community-acquired pneumonia for the treatment community-acquired pneumonia

    Institute of Scientific and Technical Information of China (English)

    曾峰; 林雅; 肖靖华; 廖美玲

    2011-01-01

    目的 探讨莫西沙星和左氧氟沙星治疗社区获得性肺炎的疗效.方法 选择社区获得性肺炎患者120例,随机分成莫西沙星组60例和左氧氟沙星组60例,分别给予治疗7d,运用药物经济学的成本-效果分析方法进行评价.结果 莫西沙星组和左氧氟沙星组治疗有效率分别为98.33%和71.67%,细菌清除率分别为91.67和65.57%,不良反应发生率分别为8.33%和13.33%,成本-效果比分别为21.87%和10.26%.结论 莫西沙星组的治疗有效率和细菌清除率明显优于左氧氟沙星组(P<0.01),莫西沙星治疗社区获得性肺炎在临床治疗中是优选方案.%Objective To study the clinical Value in moxifloxacin and levofloxacin for the treatment of community-acquired pneumonia (CAP). Methods 120 patients of CAP were chosen and divided randomly into team of moxifloxacin and team of levofloxacin. These two categories of patients were given separate treatments for 7 days which were analyzed and appraised by the cost-effect method in pharmacoeconomic research. Results The Overall clinical effective rate for team of moxifloxacin was 98.33% and for team of levofloxcine was 71. 67% ; the bacterial clearance rate for team of moxifloxacin wag 91. 67% and for team of levofloxcine was 65. 57% ; Occurrence of the adverse reaction for team moxifloxacin was 8. 33% and for team levofloxcine was 13.33% , and the cost-effectiveness ratios were 21. 87 and 10. 26. Conclusion The overall clinical effective rate and the bacterial clearance rate of team moxifloxacin is better than that of team levofloxcine. Moxifloxacin for the treatment CAP is the preferred plan in the clinic.

  18. In vitro function of random donor platelets stored for 7 days in composol platelet additive solution

    Directory of Open Access Journals (Sweden)

    Gupta Ashish

    2011-01-01

    Full Text Available Background and Aim: Platelets are routinely isolated from whole blood and stored in plasma for 5 days. This study was done to assess the in vitro function of random donor platelets stored for 7 days in composol platelet additive solution at 22°C. Materials and Methods: The study sample included 30 blood donors of both sex in State Blood Bank, C S M Medical University, Lucknow. Random donor platelets were prepared by the platelet-rich plasma method. Whole blood (350 ml was collected in anticoagulant Citrate Phosphate Dextrose Adenine triple blood bags. Random donor platelets were stored for 7 days at 22°C in platelet incubators and agitators with and without additive solution. Results: Platelet swirling was present in all the units at 22°C on day 7 with no evidence of bacterial contamination. Comparison of the mean values of platelet count, platelet factor 3, lactate dehydrogenase, pH, glucose and platelet aggregation showed no significant difference in additive solution while platelet factor 3, glucose and platelet aggregation showed significant difference (P < 0.001 on day 7 without additive solution at 22°C. Conclusion: Our study infers that the platelet viability and aggregation were the best maintained within normal levels on day 7 of storage in platelet additive solution at 22°C. Thus, we may conclude that in vitro storage of random donor platelets with an extended shelf life of 7 days using platelet additive solution may be advocated to improve the inventory of platelets.

  19. Eccentric and concentric muscle performance following 7 days of simulated weightlessness

    Science.gov (United States)

    Hayes, Judith C.; Roper, Mary L.; Mazzocca, Augustus D.; Mcbrine, John J.; Barrows, Linda H.; Harris, Bernard A.; Siconolfi, Steven F.

    1992-01-01

    Changes in skeletal muscle strength occur in response to chronic disuse or insufficient functional loading. The purpose of this study was to examine changes in muscle performance of the lower extremity and torso prior to and immediately after 7 days of simulated weightlessness (horizontal bed rest). A Biodex was used to determine concentric and eccentric peak torque and angle at peak torque for the back, abdomen, quadriceps, hamstring, soleus, and tibialis anterior. A reference angle of 0 degrees was set at full extension. Data were analyzed by ANOVA.

  20. Delayed profound thrombocytopenia presenting 7 days after use of abciximab (ReoPro).

    Science.gov (United States)

    Sharma, Sanjiv; Bhambi, Brijesh; Nyitray, William; Sharma, Geetanjali; Shambaugh, Shawn; Antonescu, Adrian; Shukla, Pankaj; Denny, Eileen

    2002-01-01

    A case of a 65-year-old woman presenting with delayed profound thrombocytopenia 7 days after the use of abciximab (ReoPro) in the setting of percutaneous coronary intervention is described. The patient had normal platelet counts for the first 24 hours after the use of abciximab (ReoPro). She presented with petechiae and profound thrombocytopenia 1 week later. The patient was treated successfully with a platelet transfusion and recovered uneventfully. Profound thrombocytopenia occurs acutely within the first few hours after abciximab (ReoPro) use, so this case was unique in that the profound thrombocytopenia presented 1 week after use of abciximab (ReoPro).

  1. Efficacy of Ciprofloxacin and Moxifloxacin against Nocardia brasiliensis In Vitro and in an Experimental Model of Actinomycetoma in BALB/c Mice▿

    OpenAIRE

    Chacon-Moreno, Brenda Edith; Welsh, Oliverio; Cavazos-Rocha, Norma; de la Luz Salazar-Cavazos, Maria; Garza-Lozano, Hector Gerardo; Said-Fernandez, Salvador; Ocampo-Candiani, Jorge; Vera-Cabrera, Lucio

    2008-01-01

    The efficacy of ciprofloxacin and moxifloxacin against Nocardia brasiliensis was evaluated by applying 25 mg of each drug/kg subcutaneously every 8 h in BALB/c mice infected with N. brasiliensis. A statistically significant difference was observed only with moxifloxacin. A moxifloxacin-trimethoprim-sulfamethoxazole combination was as active as when each compound was used alone.

  2. 荧光分析法检测牛奶中的残留莫西沙星%Determination of Moxifloxacin Residues in Milk by Fluorimetry Method

    Institute of Scientific and Technical Information of China (English)

    席会平; 师兆忠

    2012-01-01

    目的:建立测定牛奶中莫西沙星残留量的方法.方法:基于在pH 6.50的硼酸缓冲溶液中,莫西沙星可使硫化镉-牛血清白蛋白体系的荧光强度显著猝灭的原理建立了荧光分析法测定莫西沙星残留量的新方法,其中激发波长和发射波长分别为372、536 nm.结果:莫西沙星检测浓度线性范围为50~400 μg·L-1(r=0.9991),最低检测限为13.6 μg· L-1;平均回收率为93.51%,RSD均不超过1.74%(n=5).结论:该方法简便、快捷、准确,可用于残留莫西沙星的检测.%OBJECTIVE: To establish the method for the determination of moxifloxacin residues in milk. METHODS: Based on quenching acting of moxifloxacin on the fluorescence intensity emitted by the reaction of cadmium sulfide and bovine serum albumin in a B-R buffer solution (pH 6.50), new fluorimetry method for the determination of moxifloxacin residues was established. The excitation wavelength and emission wavelength were 372 nm and 536 nm. RESULTS: The linear range of moxifloxacin was 50~400 μg·L-1 (r=0.999 1) with average recovery of 93.51% (RSD≤l.74%, n=5). The detection limit was 13.6 ug·L-1. CONCLUSIONS: The method is simple, rapid and accurate, and it is suitable for the determination of moxifloxacin residues.

  3. Pulsed moxifloxacin for the prevention of exacerbations of chronic obstructive pulmonary disease: a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Rubinstein Ethan

    2010-01-01

    Full Text Available Abstract Background Acute exacerbations contribute to the morbidity and mortality associated with chronic obstructive pulmonary disease (COPD. This proof-of-concept study evaluates whether intermittent pulsed moxifloxacin treatment could reduce the frequency of these exacerbations. Methods Stable patients with COPD were randomized in a double-blind, placebo-controlled trial to receive moxifloxacin 400 mg PO once daily (N = 573 or placebo (N = 584 once a day for 5 days. Treatment was repeated every 8 weeks for a total of six courses. Patients were repeatedly assessed clinically and microbiologically during the 48-week treatment period, and for a further 24 weeks' follow-up. Results At 48 weeks the odds ratio (OR for suffering an exacerbation favoured moxifloxacin: per-protocol (PP population (N = 738, OR 0.75, 95% confidence interval (CI 0.565-0.994, p = 0.046, intent-to-treat (ITT population (N = 1149, OR 0.81, 95% CI 0.645-1.008, p = 0.059, and a post-hoc analysis of per-protocol (PP patients with purulent/mucopurulent sputum production at baseline (N = 323, OR 0.55, 95% CI 0.36-0.84, p = 0.006. There were no significant differences between moxifloxacin and placebo in any pre-specified efficacy subgroup analyses or in hospitalization rates, mortality rates, lung function or changes in St George's Respiratory Questionnaire (SGRQ total scores. There was, however, a significant difference in favour of moxifloxacin in the SGRQ symptom domain (ITT: -8.2 vs -3.8, p = 0.009; PP: -8.8 vs -4.4, p = 0.006. Moxifloxacin treatment was not associated with consistent changes in moxifloxacin susceptibility. There were more treatment-emergent, drug related adverse events with moxifloxacin vs placebo (p Conclusions Intermittent pulsed therapy with moxifloxacin reduced the odds of exacerbation by 20% in the ITT population, by 25% among the PP population and by 45% in PP patients with purulent/mucopurulent sputum at baseline. There were no unexpected adverse

  4. SRI-286, a Thiosemicarbazole, in Combination with Mefloquine and Moxifloxacin for Treatment of Murine Mycobacterium avium Complex Disease

    OpenAIRE

    Bermudez, Luiz E.; Kolonoski, Peter; Seitz, Lianne E.; Petrofsky, Mary; Reynolds, Robert; Wu, Martin; Young, Lowell S.

    2004-01-01

    Treatment of Mycobacterium avium disease remains challenging when macrolide resistance develops. We infected C57 beige mice and treated them with mefloquine, SRI-286, and moxifloxacin. SRI-286 (80 mg/kg) was bactericidal in the liver. Mefloquine plus moxifloxacin or mefloquine plus SRI-286 were better than mefloquine alone.

  5. Modeling In Vivo Pharmacokinetics and Pharmacodynamics of Moxifloxacin Therapy for Mycobacterium tuberculosis Infection by Using a Novel Cartridge System

    OpenAIRE

    Ginsburg, Amy Sarah; Lee, Jin; Woolwine, Samuel C.; Jacques H Grosset; Hamzeh, Fayez M.; Bishai, William R.

    2005-01-01

    To study the efficacy of moxifloxacin treatment for tuberculosis, we utilized a novel cartridge system to simulate in vivo pharmacokinetics. We found this system to be a robust method for modeling in vivo pharmacokinetics and present data supporting the utility of intermittent moxifloxacin treatment as a component of antituberculosis chemotherapy.

  6. No proarrhythmic properties of the antibiotics Moxifloxacin or Azithromycin in anaesthetized dogs with chronic-AV block

    DEFF Research Database (Denmark)

    Thomsen, Morten Bækgaard; Beekman, J D M; Attevelt, N J M;

    2006-01-01

    The therapeutically available quinolone antibiotic moxifloxacin has been used as a positive control for prolonging the QT interval in both clinical and non-clinical studies designed to assess the potential of new drugs to delay cardiac repolarization. Despite moxifloxacin prolonging QT, it has no...

  7. Supramolecular interaction of Moxifloxacin and β-cyclodextrin spectroscopic characterization and analytical application

    Science.gov (United States)

    Dsugi, Nuha Fathi Ali; Elbashir, Abdalla A.

    2015-02-01

    The supramolecular interaction of Moxifloxacin (Moxi) and β-cyclodextrin (β-CD) has been examined by UV-VIS, FTIR, H1NMR, SEM and fluorescence spectroscopy. The formation of inclusion complex has been confirmed on the base of changes of spectroscopy properties. The results showed that β-CD reacted with Moxi to form an inclusion complex. The Moxi and β-CD complex formed a host-guest complex in 1:1 stoichiometry and inclusion constant (K = 3.95 × 102 L mol-1) was ascertained by the typical double reciprocal plots. Furthermore, the thermodynamic parameters (ΔH°, ΔS° and ΔG°) associated with the inclusion process were also determined. Based on the significant enhancement of the fluorescence intensity of Moxi produced through complex formation, a simple, accurate, rapid and highly sensitive spectrofluorometric method for the determination of Moxi in pharmaceutical formulation was developed. The measurement of relative fluorescence intensity was carried out at 464 nm with excitation at 289 nm. The factors affecting the inclusion complex formation were studied and optimized. Under the optimum reaction conditions, linear relationships with good correlation coefficients (0.99973) were in the concentration range of 10-60 ng/mL for spectrofluorimetry. The limit of detection (LOD) was found to be 1.6 ng/mL. The proposed method was successfully applied to the analysis of Moxi in pharmaceutical preparation.

  8. Changes of human serum proteome profile during 7-day “dry” immersion

    Science.gov (United States)

    Pakharukova, N. A.; Pastushkova, L. Kh.; Larina, I. M.; Grigoriev, A. I.

    2011-05-01

    The aim of this study was to characterize changes of serum proteome profile during 7-day "dry" immersion (DI). The experiment with DI consisted of three series: control group without countermeasures (10 men), with using mechanical stimulation (6 men) and low-frequency myostimulation (5 men) as preventive means. Serum samples were fractionated using ClinProt robot (Bruker Daltonics) on magnetic beads (weak cation exchange magnetic beads—MB WCX) prior to mass-spectral profiling. It was obtained 170 peaks after fractionation of serum samples in each group. On 7th immersion day peak areas of fibrinopeptide A ( m/ z=1206; 1464), angiotensin II ( m/ z=1051), high molecular mass kininogen fragment ( m/ z=2133 Da) and C3-fragment of the complement system ( m/ z=1350 Da) were significantly decreased comparing with pre-experimental values of all experimental series. Peak areas of apolipoprotein C III ( m/ z=9419) and C4a fragment of the complement system ( m/ z=3206 Da) were increased. On 7th day of the recovery peak areas of all changed peaks were not close to pre-experimental values. This fact provided evidence of incomplete recovery of an organism after DI. The depth of the alterations had considerable individual variability. Thereby the detected changes of serum proteome profile in the experiment. They indicated a reorganization of the hormonal, immune systems and lipid metabolism. The use of myostimulation and mechanical stimulation as countermeasures partly compensated adverse effects of 7-day dry immersion on the parameters of coagulation system (fibrinopeptide A) and lipid metabolism (apolipoprotein CIII).

  9. The time course of altered brain activity during 7-day simulated microgravity

    Directory of Open Access Journals (Sweden)

    Yang eLiao

    2015-05-01

    Full Text Available Microgravity causes multiple changes in physical and mental levels in humans, which can induce performance deficiency among astronauts. Studying the variations in brain activity that occur during microgravity would help astronauts to deal with these changes. In the current study, resting-state functional magnetic resonance imaging (rs-fMRI was used to observe the variations in brain activity during a 7-day head down tilt (HDT bed rest, which is a common and reliable model for simulated microgravity. The amplitudes of low frequency fluctuation (ALFF of twenty subjects were recorded pre-head down tilt (pre-HDT, during a bed rest period (HDT0, and then each day in the HDT period (HDT1–HDT7. One-way analysis of variance of the ALFF values over these 8 days was used to test the variation across time period (P<0.05, corrected. Compared to HDT0, subjects presented lower ALFF values in the posterior cingulate cortex and higher ALFF values in the anterior cingulate cortex during the HDT period, which may partially account for the lack of cognitive flexibility and alterations in autonomic nervous system seen among astronauts in microgravity. Additionally, the observed improvement in function in CPL during the HDT period may play a compensatory role to the functional decline in the paracentral lobule to sustain normal levels of fine motor control for astronauts in a microgravity environment. Above all, those floating brain activities during 7 days of simulated microgravity may indicate that the brain self-adapts to help astronauts adjust to the multiple negative stressors encountered in a microgravity environment.

  10. Spectrophotometric Determination of Gemifloxacin Mesylate, Moxifloxacin Hydrochloride, and Enrofloxacin in Pharmaceutical Formulations Using Acid Dyes

    Directory of Open Access Journals (Sweden)

    Ayman A. Gouda

    2014-01-01

    Full Text Available Simple, rapid, and extractive spectrophotometric methods were developed for the determination of some fluoroquinolones antibiotics: gemifloxacin mesylate (GMF, moxifloxacin hydrochloride (MXF, and enrofloxacin (ENF in pure forms and pharmaceutical formulations. These methods are based on the formation of ion-pair complexes between the basic drugs and acid dyes, namely, bromocresol green (BCG, bromocresol purple (BCP, bromophenol blue (BPB, bromothymol blue (BTB, and methyl orange (MO in acidic buffer solutions. The formed complexes were extracted with chloroform and measured at 420, 408, 416, 415, and 422 nm for BCG, BCP, BPB, BTB, and MO, respectively, for GMF; at 410, 415, 416, and 420 nm for BCP, BTB, BPB, and MO, respectively, for MXF; and at 419 and 414 nm for BCG and BTB, respectively, in case of ENF. The analytical parameters and their effects are investigated. Beer’s law was obeyed in the ranges 1.0–30, 1.0–20, and 2.0–24 μg mL−1 for GMF, MXF, and ENF, respectively. The proposed methods have been applied successfully for the analysis of the studied drugs in pure forms and pharmaceutical formulations. Statistical comparison of the results with the reference methods showed excellent agreement and indicated no significant difference in accuracy and precision.

  11. Allometric scaling of marbofloxacin, moxifloxacin, danofloxacin and difloxacin pharmacokinetics: a retrospective analysis.

    Science.gov (United States)

    Cox, S K

    2007-10-01

    The purpose of this study was to examine the allometric analyses of marbofloxacin, moxifloxacin, danofloxacin and difloxacin using pharmacokinetic data from the literature. The parameters of interest (half-life, clearance and volume of distribution) were correlated across species as a function of body weight using an allometric approach (Y = aWb). Results of the allometric analysis indicated similarity between clearance and volume of distribution as they relate to body weight for all drugs. The elimination half-life was independent of body mass for all fluoroquinolones except moxifloxacin. Results of the analysis suggest that allometric scaling can be used as a tool for predicting pharmacokinetic parameters for fluoroquinolones.

  12. A validated HPTLC method for estimation of moxifloxacin hydrochloride in tablets.

    Science.gov (United States)

    Dhillon, Vandana; Chaudhary, Alok Kumar

    2010-10-01

    A simple HPTLC method having high accuracy, precision and reproducibility was developed for the routine estimation of moxifloxacin hydrochloride in the tablets available in market and was validated for various parameters according to ICH guidelines. moxifloxacin hydrochloride was estimated at 292 nm by densitometry using Silica gel 60 F254 as stationary phase and a premix of methylene chloride: methanol: strong ammonia solution and acetonitrile (10:10:5:10) as mobile phase. Method was found linear in a range of 9-54 nanograms with a correlation coefficient >0.99. The regression equation was: AUC = 65.57 × (Amount in nanograms) + 163 (r(2) = 0.9908). PMID:23781417

  13. Moxifloxacin's Limited Efficacy in the Hollow-Fiber Model of Mycobacterium abscessus Disease.

    Science.gov (United States)

    Ferro, Beatriz E; Srivastava, Shashikant; Deshpande, Devyani; Pasipanodya, Jotam G; van Soolingen, Dick; Mouton, Johan W; van Ingen, Jakko; Gumbo, Tawanda

    2016-06-01

    Current regimens used to treat pulmonary Mycobacterium abscessus disease have limited efficacy. There is an urgent need for new drugs and optimized combinations and doses. We performed hollow-fiber-system studies in which M. abscessus was exposed to moxifloxacin lung concentration-time profiles similar to human doses of between 0 and 800 mg/day. The minimum bactericidal concentration and MIC were 8 and 2 mg/liter, respectively, in our M. abscessus strain, suggesting bactericidal activity. Measurement of the moxifloxacin concentrations in each hollow-fiber system revealed an elimination rate constant (kel) of 0.11 ± 0.05 h(-1) (mean ± standard deviation) (half-life of 9.8 h). Inhibitory sigmoid maximal effect (Emax) modeling revealed that the highest Emax was 3.15 ± 1.84 log10 CFU/ml on day 3, and the exposure mediating 50% of Emax (EC50) was a 0- to 24-h area under the concentration time curve (AUC0-24)-to-MIC ratio of 41.99 ± 31.78 (r(2) = 0.99). The EC80 was an AUC0-24/MIC ratio of 102.11. However, no moxifloxacin concentration killed the bacteria to burdens below the starting inoculum. There was regrowth beyond day 3 in all doses, with replacement by a resistant subpopulation that had an MIC of >32 mg/liter by the end of the experiment. A quadratic function best described the relationship between the AUC0-24/MIC ratio and the moxifloxacin-resistant subpopulation. Monte Carlo simulations of 10,000 patients revealed that the 400- to 800-mg/day doses would achieve or exceed the EC80 in ≤12.5% of patients. The moxifloxacin susceptibility breakpoint was 0.25 mg/liter, which means that almost all M. abscessus clinical strains are moxifloxacin resistant by these criteria. While moxifloxacin's efficacy against M. abscessus was poor, formal combination therapy studies with moxifloxacin are still recommended. PMID:27067317

  14. Stability indicating HPLC method for simultaneous determination of moxifloxacin hydrochloride and ketorolac tromethamine in pharmaceutical formulations

    OpenAIRE

    Syed Naeem Razzaq; Islam Ullah Khan; Muhammad Ashfaq; Irfana Mariam

    2012-01-01

    A simple, RP-HPLC method was established for determining moxifloxacin and ketorolac in pharmaceutical formulations. Moxifloxacin, ketorolac and their degradation products were separated using C8 column with methanol and phosphate buffer pH 3.0 (55:45 v/v) as the mobile phase. Detection was performed at 243 nm using a diode array detector. The method was validated using ICH guidelines and was linear in the range 20-140 µg mL-1 for both analytes. Good separation of both the analytes and their d...

  15. A Meta-analysis of Sequential Intravenous/Oral Moxifloxacin Monotherapy for Treatment of Skin and Skin Structure Infections.

    Science.gov (United States)

    Chu, Y; Qu, J; Qu, L-Y; Luo, Y-F; Jiang, M-Y

    2015-12-01

    Moxifloxacin is widely recognized for the treatment of bacterial infections of the respiratory tract such as community acquired pneumonia, acute bacterial sinusitis and acute exacerbations of chronic bronchitis. However, the use of moxifloxacin for skin infections is much valued in recent years. This study is to compare the clinical efficacy and safety of moxifloxacin monotherapy among adults with skin and skin structure infections. The meta-analysis of RCTs is conducted by searching Medline, Embase, Pubmed and the Cochrane Library. 6 RCTs, involving a total of 2608 patients, were included in the meta-analysis. English and Chinese language papers were reviewed. The results of the meta-analysis showed that the moxifloxacin monotherapy has similar clinical cure rate, bacteriological success rates and mortality compared with the control group. The drug-related adverse of moxifloxacin was significantly higher than that in the control group, although the overall incidence of adverse events, serious adverse events, and serious drug-related adverse events were similar between the compared treatment groups. Through this meta-analysis, we can draw a conclusion that moxifloxacin monotherapy has similar effectiveness and relative safety as other recommended antibiotics for the treatment of SSSIs. At the same time, it possesses the superior bacteria eradication rate. The once-daily dosing of moxifloxacin monotherapy may be a useful alternative for other recommended antibiotic therapy. PMID:26070015

  16. Pyruvate ingestion for 7 days does not improve aerobic performance in well-trained individuals

    Science.gov (United States)

    Morrison, M. A.; Spriet, L. L.; Dyck, D. J.

    2000-01-01

    The purposes of the present studies were to test the hypotheses that lower dosages of oral pyruvate ingestion would increase blood pyruvate concentration and that the ingestion of a commonly recommended dosage of pyruvate (7 g) for 7 days would enhance performance during intense aerobic exercise in well-trained individuals. Nine recreationally active subjects (8 women, 1 man) consumed 7, 15, and 25 g of pyruvate and were monitored for a 4-h period to determine whether blood metabolites were altered. Pyruvate consumption failed to significantly elevate blood pyruvate, and it had no effect on indexes of carbohydrate (blood glucose, lactate) or lipid metabolism (blood glycerol, plasma free fatty acids). As a follow-up, we administered 7 g/day of either placebo or pyruvate, for a 1-wk period to seven, well-trained male cyclists (maximal oxygen consumption, 62.3 +/- 3.0 ml. kg(-1). min(-1)) in a randomized, double-blind, crossover trial. Subjects cycled at 74-80% of their maximal oxygen consumption until exhaustion. There was no difference in performance times between the two trials (placebo, 91 +/- 9 min; pyruvate, 88 +/- 8 min). Measured blood parameters (insulin, peptide C, glucose, lactate, glycerol, free fatty acids) were also unaffected. Our results indicate that oral pyruvate supplementation does not increase blood pyruvate content and does not enhance performance during intense exercise in well-trained cyclists.

  17. Autonomous Motivation Predicts 7-Day Physical Activity in Hong Kong Students.

    Science.gov (United States)

    Ha, Amy S; Ng, Johan Y Y

    2015-07-01

    Autonomous motivation predicts positive health behaviors such as physical activity. However, few studies have examined the relation between motivational regulations and objectively measured physical activity and sedentary behaviors. Thus, we investigated whether different motivational regulations (autonomous motivation, controlled motivation, and amotivation) predicted 7-day physical activity, sedentary behaviors, and health-related quality of life (HRQoL) of students. A total of 115 students (mean age = 11.6 years, 55.7% female) self-reported their motivational regulations and health-related quality of life. Physical activity and sedentary behaviors were measured using accelerometers for seven days. Using multilevel modeling, we found that autonomous motivation predicted higher levels of moderate-to-vigorous physical activity, less sedentary behaviors, and better HRQoL. Controlled motivation and amotivation each only negatively predicted one facet of HRQoL. Results suggested that autonomous motivation could be an important predictor of physical activity behaviors in Hong Kong students. Promotion of this form of motivational regulation may also increase HRQoL.

  18. Limited-Sampling Strategies for Therapeutic Drug Monitoring of Moxifloxacin in Patients With Tuberculosis

    NARCIS (Netherlands)

    Pranger, Arianna D.; Kosterink, Jos G. W.; van Altena, Richard; Aarnoutse, Rob E.; van der Werf, Tjip S.; Uges, Donald R. A.; Alffenaar, Jan-Willem C.

    2011-01-01

    Background: Moxifloxacin (MFX) is a potent drug for multidrug resistant tuberculosis(TB) treatment and is also useful if first-line agents are not tolerated. Therapeutic drug monitoring may help to prevent treatment failure. Obtaining a full concentration-time curve of MFX for therapeutic drug monit

  19. Optimization of separation and determination of moxifloxacin and its related substances by RP-HPLC.

    Science.gov (United States)

    Djurdjevic, Predrag; Ciric, Andrija; Djurdjevic, Aleksandra; Stankov, Milena Jelikic

    2009-09-01

    A RP-HPLC method for the separation and determination of impurities of moxifloxacin, in its pharmaceutical forms as well as moxifloxacin degradation products, was developed with the aid of DryLab software and chemometric (response surface) approach. The separation of four synthesis-related impurities was achieved on a Waters C(18) XTerra column using a mobile phase of (water+triethylamine (2%, v/v)): acetonitrile=90:10 (v/v%); the pH of water phase being adjusted with phosphoric acid to 6.0. Flow rate of the mobile phase was 1.5 ml/min and UV detection at 290 nm was employed. The column was thermostated at 45 degrees C. The resolution between the two least resolved impurity peaks was in average, R(s,min) > 1.5. Method validation parameters indicate linear dynamic range 0.2-2.0 microg/ml with LOQ ca. 0.20 microg/ml and LOD ca. 0.05 microg/ml for all analytes. The method was applied for the impurities determination in drug tablets and infusion (Avelox), Bayer AG) and for degradation products determination in a stability study of moxifloxacin. The impurity content in the tablets and infusion was quantified as 0.1% of total drug. Two degradation products were noted under hydrolytic conditions. The method can also be used for rapid and accurate quantification of moxifloxacin hydrochloride in its tablets during stability testing.

  20. Effect of norfloxacin and moxifloxacin on melanin synthesis and antioxidant enzymes activity in normal human melanocytes.

    Science.gov (United States)

    Beberok, Artur; Wrześniok, Dorota; Otręba, Michał; Miliński, Maciej; Rok, Jakub; Buszman, Ewa

    2015-03-01

    Fluoroquinolone antibiotics provide broad-spectrum coverage for a number of infectious diseases, including respiratory as well as urinary tract infections. One of the important adverse effects of these drugs is phototoxicity which introduces a serious limitation to their use. To gain insight the molecular mechanisms underlying the fluoroquinolones-induced phototoxic side effects, the impact of two fluoroquinolone derivatives with different phototoxic potential, norfloxacin and moxifloxacin, on melanogenesis and antioxidant enzymes activity in normal human melanocytes HEMa-LP was determined. Both drugs induced concentration-dependent loss in melanocytes viability. The value of EC50 for these drugs was found to be 0.5 mM. Norfloxacin and moxifloxacin suppressed melanin biosynthesis; antibiotics were shown to inhibit cellular tyrosinase activity and to reduce melanin content in melanocytes. When comparing the both analyzed fluoroquinolones, it was observed that norfloxacin possesses greater inhibitory effect on tyrosinase activity in melanocytes than moxifloxacin. The extent of oxidative stress in cells was assessed by measuring the activity of antioxidant enzymes: SOD, CAT, and GPx. It was observed that norfloxacin caused higher depletion of antioxidant status in melanocytes when compared with moxifloxacin. The obtained results give a new insight into the mechanisms of fluoroquinolones toxicity directed to pigmented tissues. Moreover, the presented differences in modulation of biochemical processes in melanocytes may be an explanation for various phototoxic activities of the analyzed fluoroquinolone derivatives in vivo.

  1. Pharmacokinetics of Moxifloxacin in Cerebrospinal Fluid and Plasma in Patients with Tuberculous Meningitis

    NARCIS (Netherlands)

    Alffenaar, J. W. C.; van Altena, R.; Bokkerink, H. J.; Luijckx, G. J.; van Soolingen, D.; Aarnoutse, R. E.; van der Werf, T. S.

    2009-01-01

    Moxifloxacin cerebrospinal fluid (CSF) penetration was evaluated by obtaining full plasma and CSF time concentration curves for 4 patients with tuberculous meningitis. The geometric mean ratio of the areas under the curve for CSF to plasma were 0.82 (range, 0.70-0.94) at 400 mg once per day and 0.71

  2. Synthesis and evaluation of {sup 99m}Tc-moxifloxacin, a potential infection specific imaging agent

    Energy Technology Data Exchange (ETDEWEB)

    Chattopadhyay, Sankha [Radiopharmaceuticals Laboratory, Board of Radiation and Isotope Technology, Variable Energy Cyclotron Centre, 1/AF, Bidhan Nagar, Kolkata 700 064 (India)], E-mail: sankha@veccal.ernet.in; Saha Das, Sujata [Radiopharmaceuticals Laboratory, Board of Radiation and Isotope Technology, Variable Energy Cyclotron Centre, 1/AF, Bidhan Nagar, Kolkata 700 064 (India); Chandra, Susmita; De, Kakali; Mishra, Mridula [Nuclear Medicine Department, Indian Institute of Chemical Biology, Kolkata (India); Ranjan Sarkar, Bharat; Sinha, Samarendu; Ganguly, Shantanu [Regional Radiation Medicine Centre, Variable Energy Cyclotron Centre, Kolkata (India)

    2010-02-15

    To synthesize and evaluate a {sup 99m}Tc labeled fluroquinolone, moxifloxacin as a potential bacteria specific infection imaging agent. A radiolabeling formulation including moxifloxacin, [Moxicip{sup TM} injection, Cipla] (4 mg), sodium pertechnetate and stannous chloride (5 {mu}g) gave the best radiolabeling efficiency and moderately stable labeled {sup 99m}Tc moxifloxacin. Quality control analysis was performed by ITLC. Rats and rabbit with infectious intramuscular lesions induced in either thigh with E. Colli were used for studying biodistribution and scintigraphic imaging of the labeled product. Imaging of an infected thigh of a rabbit was performed with a {gamma}-camera at various intervals. A good radiolabeling efficiency (90-95%) was obtained within 5 min. No purification of the labeled product was done. Labeled product retained its radiochemical purity upto 85% even at 3 h. Scintigraphy showed uptake in infectious lesions at 30 min after injection, which remains constant upto 3 h study. Abscess-to-muscle ratios were 1.60, 1.62, 1.74 and 1.75 at 30 min, 1, 2 and 3 h, respectively. Thus, {sup 99m}Tc moxifloxacin, a new potential radiopharmaceutical has been developed for infection imaging agent.

  3. Evaluation of moxifloxacin for the treatment of tuberculosis : 3 years of experience

    NARCIS (Netherlands)

    Pranger, A. D.; van Altena, R.; Aarnoutse, R. E.; van Soolingen, D.; Uges, D. R. A.; Kosterink, J. G. W.; van der Werf, T. S.; Alffenaar, J. W. C.

    2011-01-01

    Moxifloxacin (MFX) is a powerful second-line anti-tuberculosis (TB) agent, but the optimal dose has not yet been established and long-term safety data are scarce. We retrospectively reviewed the medical charts of TB patients treated at the Tuberculosis Centre Beatrixoord, University Medical Centre G

  4. Pharmacokinetics of moxifloxacin in cerebrospinal fluid and plasma in patients with tuberculous meningitis.

    NARCIS (Netherlands)

    Alffenaar, J.W.C.; Altena, R. van; Bokkerink, H.J.; Luijckx, G.J.R.; Soolingen, D. van; Aarnoutse, R.E.; Werf, T.S. van der

    2009-01-01

    Moxifloxacin cerebrospinal fluid (CSF) penetration was evaluated by obtaining full plasma and CSF time concentration curves for 4 patients with tuberculous meningitis. The geometric mean ratio of the areas under the curve for CSF to plasma were 0.82 (range, 0.70-0.94) at 400 mg once per day and 0.71

  5. Oral bioavailability of moxifloxacin after Roux-en-Y gastric bypass surgery

    NARCIS (Netherlands)

    De Smet, Julie; Colin, Pieter; De Paepe, Peter; Ruige, Johannes; Batens, Helene; Van Nieuwenhove, Yves; Vogelaers, Dirk; Blot, Stijn; Van Bocxlaer, Jan; Van Bortel, Luc M.; Boussery, Koen

    2012-01-01

    Objectives: Roux-en-Y gastric bypass surgery is the most commonly performed procedure for the treatment of morbid obesity. This anatomical alteration may affect the absorption and consequently the bioavailability of oral drugs. This study aims to investigate the oral bioavailability of moxifloxacin

  6. 莫西沙星控制结核病复发的效果分析%Analysis of the effect of moxifloxacin on tuberculosis recurrence

    Institute of Scientific and Technical Information of China (English)

    王秀丽; 汪远红; 梁肇和

    2012-01-01

    目的 分析初治结核病人强化期使用莫西沙星可减少结核病复发.方法 对2005年1月至2006年12月200例初治肺结核病人随机分为两组,每组100例,初治强化期二个月Ⅰ组用药HREZ,Ⅱ组在用HREZ基础上加用莫西沙星0.4g,每天一次,巩固期六个月均为HR,观察5年病人的复发率.结果 Ⅰ组病人100例5年内复发18例,复发率为18%,Ⅱ组病人100例5年内复发2例,复发率为2%.结论 初治病人强化期治疗使用莫西沙星能有效地控制结核病人的复发.%Objective To analyze the effects of moxifloxacin on tuberculosis recurrence patients initially treated in intensive stage. Method 200 pulmonary tuberculosis patients initially treated from January 2005 to December 2006 were randomly divided into two groups, 100 cases in each group. In the intensive period of two months, group Ⅰ received 11REZ, while group Ⅱ received moxifloxacin 0. 4g qd based on the treatment of groupⅠ In consolidation period of six months, two groups all received 11REZ. Relapse rate of 5 years was observed in two groups. Result 18 cases recurred in group Ⅰ , and relapse rate of 5 years was 18% ; 2 cases recurred in group Ⅰ , and relapse rate of 5 years was 2% . Conclusion Moxifloxacin can effectively control the recurrence of tuberculosis patients initially treated in intensive stage.

  7. In vitro activity of moxifloxacin and piperacillin/sulbactam against pathogens of acute cholangitis

    Institute of Scientific and Technical Information of China (English)

    Andreas Weber; Wolfgang Huber; Klaus Kamereck; Philipp Winkle; Petra Voland; Hans Weidenbach; Roland M Schmid; Christian Prinz

    2008-01-01

    AIM:To analyze the in vitro activity of moxifioxacin and piperacillin/sulbactam against pathogens isolated from patients with acute cholangitis.METHODS:In this prospective study a total of 65 patients with acute cholangitis due to biliary stone obstruction (n = 7),benign biliary stricture (n =16),and malignant biliary stricture (n = 42) were investigated with regard to spectrum of bacterial infection and antibiotic resistance.Pathogens were isolated from bile cultures in all study patients.In 22 febrile patients,blood cultures were also obtained.In vitro activity of moxifloxacin and piperacillin/sulbactam was determined by agar diffusion.RESULTS:Thirty-one out of 65 patients had positive bile and/or blood cultures.In 31 patients,63 isolates with 17 different species were identified.The predominant strains were Enterococcus species (26/63),Ecoli (13/63) and Klebsiella species (8/63).A comparable in vitro activity of moxifloxacin and piperacillin/sulbactam was observed for E.coli and Klebsiella species.In contrast,Enterococcus species had higher resistances towards moxifloxacin.Overall bacteria showed antibiotic resistances in vitro of 34.9% for piperacillin/sulbactam and 36.5% for moxifloxacin.CONCLUSION:Enterococcus species,E.coli andKlebsiella species were the most common bacteria isolated from bile and/or blood from patients with acute cholangitis.Overall,a mixed infection with several species was observed,and bacteria showed a comparable in vitro activity for piperacillin/sulbactam and moxifloxacin.

  8. Intensified regimen containing rifampicin and moxifloxacin for tuberculous meningitis: an open-label, randomised controlled phase 2 trial.

    NARCIS (Netherlands)

    Ruslami, R.; Ganiem, A.R.; Dian, S.; Apriani, L.; Achmad, T.H.; Ven, A.J.A.M. van der; Borm, G.F.; Aarnoutse, R.E.; Crevel, R. van

    2013-01-01

    BACKGROUND: Intensified antibiotic treatment might improve the outcome of tuberculous meningitis. We assessed pharmacokinetics, safety, and survival benefit of several treatment regimens containing high-dose rifampicin and moxifloxacin in patients with tuberculous meningitis in a hospital setting. M

  9. Comparison of aqueous humour concentration after single high dose versus multiple administration of topical moxifloxacin in rabbits

    OpenAIRE

    Monika Chopra; Rehan, H. S.; Rachna Gupta; Ahmad, F. J.; M D Tariq; Gupta, L. K.

    2014-01-01

    For the prevention of postoperative ocular infections prophylactic topical antibiotics are routinely used. Studies evaluating comparative difference between single dose versus multiple dose administration on aqueous humour concentration of moxifloxacin are lacking. This study compared the aqueous humour concentration of moxifloxacin following its topical administration in rabbit eyes with two dose regimens. Twelve albino rabbits were divided into two groups. In group-1, two drops were adminis...

  10. Comparative evaluation of aqueous and plasma concentration of topical moxifloxacin alone and with flurbiprofen in patients of cataract surgery

    OpenAIRE

    Sujash Halder; Kanchan Kumar Mondal; Supreeti Biswas; Tapan Kumar Mandal; Bakul Kumar Dutta; Mithilesh Haldar

    2013-01-01

    Objectives: To determine the aqueous and plasma concentrations of moxifloxacin administered topically alone and with flurbiprofen in patients undergoing cataract surgery. Materials and Methods: A total of 50 subjects scheduled for routine cataract surgery were randomly allocated to two groups (n = 25 each). Group-1 patients were treated with topical moxifloxacin alone: One drop 6 times/day for 3 days before surgery and one drop 4 times on the day of surgery: Group-2 patients were treated ...

  11. Efficacy and Safety of Intravenous Moxifloxacin Versus Cefoperazone with Azithromycin in the Treatment of Community Acquired Pneumonia

    Institute of Scientific and Technical Information of China (English)

    XU Shuyun; XIONG Shengdao; XU Yongjian; LIU Jin; LIU Huiguo; ZHAO Jianping; XIONG Weining

    2006-01-01

    To compare the efficacy, safety, and tolerability of intravenous moxifloxacin with those of a commonly used empirical antibiotic regimen, cefoperazone and azithromycin in the treatment of community acquired pneumonia (CAP) in adult patients requiring initial parenteral therapy, 40 patients with CAP were divided into two groups, a moxifloxacin group (n=20) and a control group(n=20), which were treated for 7 to 14 days. The patients in the moxifloxacin group were intravenously given 400 mg of moxifloxacin (AveloxR) once a day. Patients in the control group were administered 2.0 g of cefoperazone twice a day and azithromycin 0.5 g once a day. Clinical, bacteriological, and laboratory examinations were performed before the treatment, and at the end of the treatment. Our results showed that there was no significant difference in the clinical efficacy rate between two treatment groups at end of therapy (90 % for moxifloxacin, 95 % for cefoperazone plus azithromycin) (P>0.05). The bacteriologic eradication rate at the end of treatment was 90 % in the moxifloxacin group and 80 % in the cefoperazone-plus-azithromycin group, whereas there was no significant difference between the two groups (P>0.05). In addition, both drugs were well-tolerated in this trial, with the number of drug-related adverse events being comparable. It is concluded that moxifloxacin is an effective and well-tolerated treatment for CAP and was equivalent to the commonly used empirical treatment of cefoperazone plus azithromycin. Moxifloxacin is likely to offer clinicians an alternative for reliable empirical CAP treatment in the face of increasing antibiotic resistance.

  12. [In vitro activity of linezolid, moxifloxacin, levofloxacin, clindamycin and rifampin, alone and in combination, against Staphylococcus aureus and Staphylococcus epidermidis].

    Science.gov (United States)

    Soriano, A; Jurado, A; Marco, F; Almela, M; Ortega, M; Mensa, J

    2005-06-01

    Information about the in vitro effect of combinations of anti-staphylococcal agents on staphylococci is scarce. The aim of the study was to evaluate the in vitro activity of linezolid, moxifloxacin, levofloxacin, clindamycin and rifampin, alone or in combination, against Staphylococcus spp. Two Staphylococcus aureus and two Staphylococcus epidermidis strains isolated from blood cultures were studied using the killing curve method. The combinations analyzed were linezolid+moxifloxacin, linezolid+levofloxacin, linezolid+clindamycin, linezolid+rifampin, moxifloxacin+rifampin, moxifloxacin+clindamycin, levofloxacin+rifampin and levofloxacin+clindamycin. The following concentrations (mg/l) were used: 8 and 16 for linezolid, 2 for moxifloxacin, 3 for levofloxacin, 2 for clindamycin and 2 and 5 for rifampin. The activity was considered synergistic when a reduction in growth of at least 2 log(10) was produced with the combination in comparison to the most active antibiotic alone; antagonistic when a growth of at least 2 log(10) was produced with the combination in comparison to the most active antibiotic alone; and indifferent if the variation was less than 1 log(10). Linezolid and clindamycin were bacteriostatic, while moxifloxacin and levofloxacin were bactericidal. Rifampin was bacteriostatic against S. aureus and bactericidal against S. epidermidis. Linezolid and clindamycin reduced the bactericidal activity of levofloxacin and moxifloxacin, however an antagonistic effect was only observed against S. aureus. Other combinations of linezolid, rifampin, clindamycin, levofloxacin or moxifloxacin were indifferent. Linezolid and clindamycin antagonize the bactericidal activity of fluorquinolones against staphylococci. There was no difference between any other combinations against either S. aureus or S. epidermidis.

  13. Comparison of aqueous humour concentration after single high dose versus multiple administration of topical moxifloxacin in rabbits

    Directory of Open Access Journals (Sweden)

    Monika Chopra

    2014-01-01

    Full Text Available For the prevention of postoperative ocular infections prophylactic topical antibiotics are routinely used. Studies evaluating comparative difference between single dose versus multiple dose administration on aqueous humour concentration of moxifloxacin are lacking. This study compared the aqueous humour concentration of moxifloxacin following its topical administration in rabbit eyes with two dose regimens. Twelve albino rabbits were divided into two groups. In group-1, two drops were administered thrice (total six drops at 2 min intervals, in both the eyes; in group-2, two drops of moxifloxacin were administered three times a day for three days and also two h before aqueous humour collection i.e. on fourth day. Mean aqueous humour concentrations were calculated and compared using Student′s ′t′ test and P<0.05 was considered significant. Moxifloxacin concentration in aqueous humour in group-1 was 23.79 μg/ml and in group-2 was 42.08 μg/ml. Both dosing regimens produced substantially higher aqueous concentrations than the known minimum inhibitory concentration for most bacteria. Moxifloxacin concentration in aqueous humour with multiple instillations is significantly higher than single instillation (P<0.05, which is adequate to cover ciprofloxacin-resistant gram-negative bacteria. Repeated topical moxifloxacin administration achieved significantly higher aqueous humour concentrations than single administration.

  14. Comparative evaluation of aqueous and plasma concentration of topical moxifloxacin alone and with flurbiprofen in patients of cataract surgery

    Science.gov (United States)

    Halder, Sujash; Mondal, Kanchan Kumar; Biswas, Supreeti; Mandal, Tapan Kumar; Dutta, Bakul Kumar; Haldar, Mithilesh

    2013-01-01

    Objectives: To determine the aqueous and plasma concentrations of moxifloxacin administered topically alone and with flurbiprofen in patients undergoing cataract surgery. Materials and Methods: A total of 50 subjects scheduled for routine cataract surgery were randomly allocated to two groups (n = 25 each). Group-1 patients were treated with topical moxifloxacin alone: One drop 6 times/day for 3 days before surgery and one drop 4 times on the day of surgery: Group-2 patients were treated with topical moxifloxacin as in Group-1 and with topical flurbiprofen: One drop 4 times/day for 3 days before and on the day of surgery. The interval between two drugs was 30 min for last 3 days and 15 min on the day of surgery. Last dose was administered 1 h before aqueous humor and blood sampling for both the groups. The antibiotic concentration in aqueous humor and plasma were determined by using high performance liquid chromatography. Results: The mean concentration of moxifloxacin in aqueous humor was 1.71 ± 0.82 mg/ml in Group-1 and 2.39 ± 1.34 mg/ml in Group-2. Concentrations of moxifloxacin in aqueous humor were significantly higher in Group-2 than that of Group-1. Conclusion: Flurbiprofen may increase the concentration of moxifloxacin in aqueous humor. PMID:23833362

  15. Comparative evaluation of aqueous and plasma concentration of topical moxifloxacin alone and with flurbiprofen in patients of cataract surgery

    Directory of Open Access Journals (Sweden)

    Sujash Halder

    2013-01-01

    Full Text Available Objectives: To determine the aqueous and plasma concentrations of moxifloxacin administered topically alone and with flurbiprofen in patients undergoing cataract surgery. Materials and Methods: A total of 50 subjects scheduled for routine cataract surgery were randomly allocated to two groups (n = 25 each. Group-1 patients were treated with topical moxifloxacin alone: One drop 6 times/day for 3 days before surgery and one drop 4 times on the day of surgery: Group-2 patients were treated with topical moxifloxacin as in Group-1 and with topical flurbiprofen: One drop 4 times/day for 3 days before and on the day of surgery. The interval between two drugs was 30 min for last 3 days and 15 min on the day of surgery. Last dose was administered 1 h before aqueous humor and blood sampling for both the groups. The antibiotic concentration in aqueous humor and plasma were determined by using high performance liquid chromatography. Results: The mean concentration of moxifloxacin in aqueous humor was 1.71 ± 0.82 mg/ml in Group-1 and 2.39 ± 1.34 mg/ml in Group-2. Concentrations of moxifloxacin in aqueous humor were significantly higher in Group-2 than that of Group-1. Conclusion: Flurbiprofen may increase the concentration of moxifloxacin in aqueous humor.

  16. Comparative Efficacy and Safety of Moxifloxacin and Clindamycin in the Treatment of Odontogenic Abscesses and Inflammatory Infiltrates: a Phase II, Double-Blind, Randomized Trial▿

    OpenAIRE

    Cachovan, Georg; Böger, Rainer H.; Giersdorf, Ina; Hallier, Olaf; Streichert, Thomas; Haddad, Munif; Platzer, Ursula; Schön, Gerhard; Wegscheider, Karl; Sobottka, Ingo

    2010-01-01

    Moxifloxacin penetrates well into oromaxillary tissue and covers the causative pathogens that show an increasing resistance to standard antibiotics. Clinical reports suggest that moxifloxacin may be effective for the treatment of odontogenic infections that can lead to serious complications. The objective of this prospective, randomized, double-blind, multicenter study was to compare the efficacies and safeties of moxifloxacin and clindamycin for the medical treatment of patients with gingiva...

  17. Disposition kinetics of long acting moxifloxacin following intravenous administration in Sheep

    Directory of Open Access Journals (Sweden)

    Chirag M. Modi

    Full Text Available Aim: The objective of the present study was to study the disposition kinetics and dosage regimens of long acting moxifloxacin following intravenous administration at the dose rate of 7.5 mg/kg-1 b. wt. in six male sheep and to calculate dosage regimens of the same in sheep. Materials and Methods: The study was conducted using six healthy male sheep. Long acting Moxifloxacin solution (10 % moxifloxacin in solution with L- arginine, N-butyl alcohol and benzyl alcohol was injected in jugular vein and periodical blood samples were collected from contra-lateral jugular vein in test tubes containing 30-50 IU heparin (anticoagulant at 0.083 (5 min, 0.166 (10 min, 0.5, 1, 2, 4, 8, 12, 24, 36, 48, 60, 72 and up to 96 h post administration of drug. Drug concentration in plasma was determined using High Performance Liquid Chromatography (HPLC with Fluorescence Detector. The blood concentrations versus time data were analyzed using software. Results: After single dose intravenous administration of long acting moxifloxacin the plasma concentration of 0.016 ± 0.001 μg/ml-1 was maintained for up to 72 h. Distribution half-life (t and elimination half-life (t were 1.637 ± 0.053 h, and 1/2 1/2 12.130 ± 0.202 h, following IV administration. The mean values of apparent volume of distribution V 5.436 ± 0.135 L/kg-1 d(area as well as mean residence time 10.02 ± 4.787 minute were detected with IV administration. Conclusion: The long acting Moxifloxacin @ the dose 7.5 mg/kg IV maintains the effective therapeutic concentration in the plasma of sheep for up to 72 hours. The long acting Moxifloxacin at this dose rate can be used to treat sensitive bacteria causing infectious diseases in sheep. [Vet World 2012; 5(9.000: 517-521

  18. Activity of Moxifloxacin by Itself and in Combination with Ethambutol, Rifabutin, and Azithromycin In Vitro and In Vivo against Mycobacterium avium

    OpenAIRE

    Bermudez, Luiz E.; Inderlied, Clark B.; Kolonoski, Peter; Petrofsky, Mary; Aralar, Priscilla; Wu, Martin; Young, Lowell S.

    2001-01-01

    Moxifloxacin activity against Mycobacterium avium complex (MAC) was evaluated in vitro against 25 strains. The MIC was determined to range from 0.125 to 2.0 μg/ml. In addition, U937 macrophage monolayers infected with MAC strain 101 (serovar 1) were treated with moxifloxacin (0.25 to 8 μg/ml) daily, and the number of intracellular bacteria was quantitated after 4 days. Moxifloxacin showed inhibitory activity at 0.5 μg/ml and higher. To assess the activity of moxifloxacin containing regimens i...

  19. Anstieg der Clostridium difficile-assoziierten Diarrhoe nach Einsatz von Moxifloxacin

    OpenAIRE

    Günther, Claudia

    2010-01-01

    The Clostridium-difficile associated diarrhea has shown a rising incidence in recent years. This has a dramatic medial and economical impact. The main cause for the disease is a recent antibiotic therapy. There have been numerous studies which have established certain antibiotics most potent for causing colitis, eg. Cephalosporins of group II an III, clindamycin and ciprofloxacin. Moxifloxacin was introduced as a new flourochinolon a few years ago. With a high bioavailability it has s...

  20. Moxifloxacin in the management of exacerbations of chronic bronchitis and COPD

    OpenAIRE

    Miravitlles, Marc

    2007-01-01

    Bacteria are isolated in more than 50% of exacerbations of chronic bronchitis (CB) and chronic obstructive pulmonary disease (COPD). The most prevalent respiratory pathogens include Gram-positive (Streptococcus pneumoniae) and Gram-negative (Haemophilus influenzae, Moraxella catarrhalis) microorganims. Moxifloxacin is a fourth-generation fluoroquinolone that has been shown to be effective against respiratory pathogens, including atypicals and those resistant to most common antibiotics. The bi...

  1. Validated spectrophotometric methods for the estimation of moxifloxacin in bulk and pharmaceutical formulations

    Science.gov (United States)

    Motwani, Sanjay K.; Chopra, Shruti; Ahmad, Farhan J.; Khar, Roop K.

    2007-10-01

    New, simple, cost effective, accurate and reproducible UV-spectrophotometric methods were developed and validated for the estimation of moxifloxacin in bulk and pharmaceutical formulations. Moxifloxacin was estimated at 296 nm in 0.1N hydrochloric acid (pH 1.2) and at 289 nm in phosphate buffer (pH 7.4). Beer's law was obeyed in the concentration range of 1-12 μg ml -1 ( r2 = 0.9999) in hydrochloric acid and 1-14 μg ml -1 ( r2 = 0.9998) in the phosphate buffer medium. The apparent molar absorptivity and Sandell's sensitivity coefficient were found to be 4.63 × 10 4 l mol -1 cm -1 and 9.5 ng cm -2/0.001 A in hydrochloric acid; and 4.08 × 10 4 l mol -1 cm -1 and 10.8 ng cm -2/0.001 A in phosphate buffer media, respectively indicating the high sensitivity of the proposed methods. These methods were tested and validated for various parameters according to ICH guidelines. The detection and quantitation limits were found to be 0.0402, 0.1217 μg ml -1 in hydrochloric acid and 0.0384, 0.1163 μg ml -1 in phosphate buffer medium, respectively. The proposed methods were successfully applied for the determination of moxifloxacin in pharmaceutical formulations (tablets, i.v. infusions, eye drops and polymeric nanoparticles). The results demonstrated that the procedure is accurate, precise and reproducible (relative standard deviation moxifloxacin in different dosage forms and dissolution studies.

  2. Reproductive and Developmental Effects of Moxifloxacin on Female Mice and Embryos

    Directory of Open Access Journals (Sweden)

    Hanaa M. Roshdy

    2004-12-01

    Full Text Available Moxifloxacin (Avelox® is a fluoroquinolone antibiotic with a broad spectrum of activity and bactericidal action. Moxifloxacin has in vitro activity against a wide range of Gram-positive and Gram-negative organisms. The safe use of moxifloxacin in human pregnancy has not been established. In order to evaluate the genotoxic and embryo toxic effects of (Avelox® during pregnancy, Avelox was administrated orally to female mice with doses (8.7, 17 and 26 mg/kg/day from 1 to 17 days of pregnancy. Caesarean sections were completed on gestation day 18 and complete fetal examinations and cytogenetic analysis were conducted. Decreases in the fetal body weights and increases in the external visceral and skeletal anomalies were found in all doses of (8.7, 17 and 26 mg Avelox compared to the controls. Cytogenetic analysis in mothers and embryos revealed that all the tests doses produced chromosomal aberrations and micronuclei (MN formations in a dose dependent manner compared to the controls. These results indicate that Avelox has a maternal and embryotoxic effects on the female mice and their embryos when administered with a recommended and above the recommended dose during pregnancy.

  3. Synthesis and in vitro Antimycobacterial Activity of Moxifloxacin Methylene and Ethylene Isatin Derivatives

    Institute of Scientific and Technical Information of China (English)

    FENG Lian-shun; LIU Ming-liang; WANG Shuo; CHAI Yun; LI Su-jie; GUO Hui-yuan

    2012-01-01

    A series of novel moxifloxacin methylene and ethylene isatin derivatives with remarkable improvement in lipophilicity,compared to the parent moxifloxacin,was designed,synthesized and characterized by 1H NMR,MS and HRMS.These derivatives were initially evaluated for their in vitro antimycobacterial activity against M.smegmatis CMCC 93202.Compounds 3a-3f,5a,5f and 5j were chosen for the further evaluation of their in vitro activity against Mycobacterium tuberculosis(MTB) H37Rv ATCC 27294 and MDR-MTB 09710.All the target compounds[minimum inhibitory concentration(M1C):0.39->16 μg/mL] were far more active than rifampin(MIC:2.0->256 μg/mL),but less active than moxifloxacin(MIC:0.1-1.0 μg/mL) against the three tested strains.The most active compounds 3a and 3c were found to be 2-64 fold more potent than isoniazid and rifampin against M.smegmatis CMCC 93202,2 fold more potent than rifampin against MTB H37Rv ATCC 27294,and 16->64 fold more potent than ethambutol,isoniazid and rifampin against MDR-MTB 09710.

  4. Clinical efficacy and safety of moxifloxacin versus levofloxacin plus metronidazole for community-acquired pneumonia with aspiration factors

    Institute of Scientific and Technical Information of China (English)

    Sun Tieying; Sun Li; Wang Rongmei; Ren Xiaoping; Sui Dong-jiang; Pu Chun; Ren Yajuan

    2014-01-01

    Background Community-acquired pneumonia (CAP) is a common infectious disease throughout the world and the incidence continues to grow as the population ages.Aspiration is an important pathogenic mechanism for pneumonia in the elderly and the management of patients with community-acquired pneumonia with aspiration factors is a major medical problem.Our study aimed to assess whether moxifloxacin in comparison to levofloxacin plus metronidazole are effective and safe in the treatment of community-acquired pneumonia with aspiration factors.Methods In this prospective,multicenter,open-label,randomized controlled trial,77 patients with mild-to-moderate community-acquired pneumonia with aspiration factors were enrolled and randomly assigned to receive moxifloxacin or levofloxacin plus metronidazole.The primary efficacy variables were clinical outcomes in evaluable patients at a follow-up visit 7 to 14 days after the end of therapy.Results Seven days after the end of therapy a clinical cure was achieved for 76.7% (23 of 37) of efficacy-evaluable patients in the moxifloxacin group and 51.7% (15 of 40) of patients in the levofloxacin plus metronidazole group.There was a significant difference between the two groups (x2=4.002,P <0.05).Bacteriological success rates were similar in the moxifloxacin group (93.3%) and levofloxacin plus metronidazole group (96.4%),there was no significant difference between the two groups (P >0.05).The overall adverse event rate was 10.8% (4/37) in the moxifloxacin group versus 17.5% (7/40) in the levofloxacin plus metronidazole group,there was no significant difference between the two groups (P>0.05).No serious adverse events were observed.Conclusions Moxifloxacin is effective and safe for treatment of community-acquired pneumonia with aspiration factors.And the regimen of moxifloxacin monotherapy is more convenient compared with levofloxacin plus metronidazole.

  5. In Vitro Activities of Moxifloxacin against 900 Aerobic and Anaerobic Surgical Isolates from Patients with Intra-Abdominal and Diabetic Foot Infections

    OpenAIRE

    Edmiston, Charles E.; Krepel, Candace J.; Seabrook, Gary R.; Somberg, Lewis R.; Nakeeb, Atilla; Cambria, Robert A.; Towne, Jonathan B.

    2004-01-01

    The in vitro activities of moxifloxacin, ciprofloxacin, levofloxacin, gatifloxacin, imipenem, piperacillin-tazobactam, clindamycin, and metronidazole against 900 surgical isolates were determined using NCCLS testing methods. Moxifloxacin exhibited good to excellent antimicrobial activity against most aerobic (90.8%) and anaerobic (97.1%) microorganisms, suggesting that it may be effective for the treatment of polymicrobial surgical infections.

  6. Moxifloxacin and Azithromycin but not Amoxicillin Protect Human Respiratory Epithelial Cells against Streptococcus pneumoniae In Vitro when Administered up to 6 Hours after Challenge

    OpenAIRE

    Ulrich, Martina; Albers, Cordula; Möller, Jan-Georg; Dalhoff, Axel; Korfmann, Gisela; Künkele, Frank; Döring, Gerd

    2005-01-01

    We determined the protective effect of moxifloxacin, azithromycin, and amoxicillin against Streptococcus pneumoniae infection of respiratory cells. Moxifloxacin and azithromycin effectively killed intracellular S. pneumoniae strains and protected respiratory epithelial cells significantly even when given 6 h after S. pneumoniae challenge. Amoxicillin was less effective.

  7. Diabetes, hypertension, overweight and hyperlipidemia and 7-day case-fatality in first myocardial infarction

    Directory of Open Access Journals (Sweden)

    H.K. Quintana

    2016-09-01

    Conclusions: In this population-based inception cohort study, diabetes but not hypertension and hyperlipidemia were associated with MI fatality. This further emphasizes the importance of diabetes as a cardiovascular risk factor and the need for close surveillance of diabetic patients. Overweight was however associated with decreased MI fatality.

  8. EPR characteristics of free radicals in DOPA-melanin-moxifloxacin complexes at ambient level of UVA radiation

    Science.gov (United States)

    Beberok, Artur; Zdybel, Magdalena; Pilawa, Barbara; Buszman, Ewa; Wrześniok, Dorota

    2014-01-01

    EPR studies pointed out that o-semiquinone free radicals with g-values 2.0038-2.0040 take part in moxifloxacin-melanin complex formation. The process contributed to increase in free radicals concentration in nonirradiated complexes. This effect was observed for the complexes with 1 × 10-4 M, 1 × 10-3 M and 4 × 10-3 M drug concentrations. UV irradiation contributed to decrease in free radicals concentration in DOPA-melanin complexes with moxifloxacin, besides the complexes with the drug concentration of 1 × 10-4 M. The strongest decrease was observed for DOPA-melanin-moxifloxacin complexes with the drug concentration of 1 × 10-3 M. Homogeneous broadening of EPR lines, strong dipolar interactions and slow spin-lattice relaxation processes characterized all the tested melanin samples.

  9. Moxifloxacin-Gelrite in situ ophthalmic gelling system against photodynamic therapy for treatment of bacterial corneal inflammation.

    Science.gov (United States)

    El-Laithy, Hanan M; Nesseem, Demiana I; El-Adly, Amira A; Shoukry, Meriana

    2011-10-01

    In this study, six in situ gelling formulations based on Gelrite were prepared and evaluated for the retained ophthalmic delivery of Moxifloxacin (Mox). The effectiveness of the best developed formula G5 was compared with photodynamic therapy (PDT), the recent expanding approach for the treatment of ophthalmologic disorders after the assessment of optimum photodynamic inactivation parameters that permit efficient pathogens eradication. It was found that, Staphylococcus aureus (S. aureus) (Gram-positive) was more susceptible to effective lethal photosensitization that reaches 93.5% reduction in viable count than Escherichia coli (E. coli) (Gramnegative) of 76.1% using 3 mg/mL Hematoporphyrin (HP), illuminated by 630 nm Light Emitting Diode (LED) at 9 J/cm(2) and incubated for 15 min. Following topical instillation of G5 to rabbits corneas, higher amount of Mox was retained in the aqueous humor up to 24 h with significant 6-fold increase in the C(max) and AUC((0-∞)) compared to vigamox commercial eye drops. After post corneal infection with S. aureus, both approaches were effectively treating the infection without causing ocular irritation or collateral damage to corneal tissue where G5 showed remarkable improvement after four days compared to seven days of PDT treatment.

  10. Correlation between GyrA Substitutions and Ofloxacin, Levofloxacin, and Moxifloxacin Cross-Resistance in Mycobacterium tuberculosis

    Science.gov (United States)

    Willby, Melisa; Sikes, R. David; Malik, Seidu; Metchock, Beverly

    2015-01-01

    The newer fluoroquinolones moxifloxacin (MXF) and levofloxacin (LVX) are becoming more common components of tuberculosis (TB) treatment regimens. However, the critical concentrations for testing susceptibility of Mycobacterium tuberculosis to MXF and LVX are not yet well established. Additionally, the degree of cross-resistance between ofloxacin (OFX) and these newer fluoroquinolones has not been thoroughly investigated. In this study, the MICs for MXF and LVX and susceptibility to the critical concentration of OFX were determined using the agar proportion method for 133 isolates of M. tuberculosis. Most isolates resistant to OFX had LVX MICs of >1 μg/ml and MXF MICs of >0.5 μg/ml. The presence of mutations within the gyrA quinolone resistance-determining regions (QRDR) correlated well with increased MICs, and the level of LVX and MXF resistance was dependent on the specific gyrA mutation present. Substitutions Ala90Val, Asp94Ala, and Asp94Tyr resulted in low-level MXF resistance (MICs were >0.5 but ≤2 μg/ml), while other mutations led to MXF MICs of >2 μg/ml. Based on these results, a critical concentration of 1 μg/ml is suggested for LVX and 0.5 μg/ml for MXF drug susceptibility testing by agar proportion with reflex testing for MXF at 2 μg/ml. PMID:26100699

  11. Biopsy Specimens Obtained 7 Days After Starting Chemoradiotherapy (CRT) Provide Reliable Predictors of Response to CRT for Rectal Cancer

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Toshiyuki [Department of Surgery, Tokai University School of Medicine, Kanagawa (Japan); Sadahiro, Sotaro, E-mail: sadahiro@is.icc.u-tokai.ac.jp [Department of Surgery, Tokai University School of Medicine, Kanagawa (Japan); Tanaka, Akira; Okada, Kazutake; Kamata, Hiroko; Kamijo, Akemi [Department of Surgery, Tokai University School of Medicine, Kanagawa (Japan); Murayama, Chieko [Department of Clinical Pharmacology, Tokai University School of Medicine, Kanagawa (Japan); Akiba, Takeshi; Kawada, Shuichi [Department of Radiology, Tokai University School of Medicine, Kanagawa (Japan)

    2013-04-01

    Purpose: Preoperative chemoradiation therapy (CRT) significantly decreases local recurrence in locally advanced rectal cancer. Various biomarkers in biopsy specimens obtained before CRT have been proposed as predictors of response. However, reliable biomarkers remain to be established. Methods and Materials: The study group comprised 101 consecutive patients with locally advanced rectal cancer who received preoperative CRT with oral uracil/tegafur (UFT) or S-1. We evaluated histologic findings on hematoxylin and eosin (H and E) staining and immunohistochemical expressions of Ki67, p53, p21, and apoptosis in biopsy specimens obtained before CRT and 7 days after starting CRT. These findings were contrasted with the histologic response and the degree of tumor shrinkage. Results: In biopsy specimens obtained before CRT, histologic marked regression according to the Japanese Classification of Colorectal Carcinoma (JCCC) criteria and the degree of tumor shrinkage on barium enema examination (BE) were significantly greater in patients with p21-positive tumors than in those with p21-negative tumors (P=.04 and P<.01, respectively). In biopsy specimens obtained 7 days after starting CRT, pathologic complete response, histologic marked regression according to both the tumor regression criteria and JCCC criteria, and T downstaging were significantly greater in patients with apoptosis-positive and p21-positive tumors than in those with apoptosis-negative (P<.01, P=.02, P=.01, and P<.01, respectively) or p21-negative tumors (P=.03, P<.01, P<.01, and P=.02, respectively). The degree of tumor shrinkage on both BE as well as MRI was significantly greater in patients with apoptosis-positive and with p21-positive tumors than in those with apoptosis-negative or p21-negative tumors, respectively. Histologic changes in H and E-stained biopsy specimens 7 days after starting CRT significantly correlated with pathologic complete response and marked regression on both JCCC and tumor

  12. Impact of moxifloxacin on serum free amino acid and cytokines in patients with tuberculosis treated by

    Institute of Scientific and Technical Information of China (English)

    Zhao-Zhi Wang

    2015-01-01

    Objective:To explore the impact of serum free amino acid and cytokines in patients with tuberculosis treated by Moxifloxacin.Methods:Chose 130 cases pulmonary tuberculosis patients,they were divided into observation group and control group according to random number table method, 65 cases in each group, all patients were given tuberculosis standard treatment, and on this basis, patients in control group were given levofloxacin tablets, patients in observation group were given moxifloxacin hydrochloride oral, they were treated for 6 months, compared the serum free amino acid and cytokine interleukin-18 (IL-18), tumor necrosis factor alpha-gamma (TNF-α), interferon (IFN-γ), interleukin-10 (IL-10) between two groups before and after treatment. The serum isoleucine, phenylalanine, threonine, leucine, valine, aspartic acid, glutamic acid and arginine in two groups after were increased significantly than before treatment, the difference was statistically significant, acquired aspartic acid, glutamic acid in observation group after treatment were significantly higher than the control group after treatment, the difference was statistically significant; The serum IL-18, IFN-γ, TNF-α and IL-10 in two groups after treatment were significantly reduced, the difference was statistically significant (P<0.05); the serum IL-18, IFN-γ, TNF-α and IL-10 in observation group after treatment were significantly lower than the control group after treatment, the difference was statistically significant.Conclusion:Compared with levofloxacin, moxifloxacin treatment tuberculosis can improve the patients' serum free amino acid levels, adjust the Th1/Th2 balance.

  13. Reactive Oxygen Species Contribute to the Bactericidal Effects of the Fluoroquinolone Moxifloxacin in Streptococcus pneumoniae.

    Science.gov (United States)

    Ferrándiz, M J; Martín-Galiano, A J; Arnanz, C; Zimmerman, T; de la Campa, A G

    2016-01-01

    We studied the transcriptomic response of Streptococcus pneumoniae to the fluoroquinolone moxifloxacin at a concentration that inhibits DNA gyrase. Treatment of the wild-type strain R6, at a concentration of 10× the MIC, triggered a response involving 132 genes after 30 min of treatment. Genes from several metabolic pathways involved in the production of pyruvate were upregulated. These included 3 glycolytic enzymes, which ultimately convert fructose 6-phosphate to pyruvate, and 2 enzymes that funnel phosphate sugars into the glycolytic pathway. In addition, acetyl coenzyme A (acetyl-CoA) carboxylase was downregulated, likely leading to an increase in acetyl-CoA. When coupled with an upregulation in formate acetyltransferase, an increase in acetyl-CoA would raise the production of pyruvate. Since pyruvate is converted by pyruvate oxidase (SpxB) into hydrogen peroxide (H2O2), an increase in pyruvate would augment intracellular H2O2. Here, we confirm a 21-fold increase in the production of H2O2 and a 55-fold increase in the amount of hydroxyl radical in cultures treated during 4 h with moxifloxacin. This increase in hydroxyl radical through the Fenton reaction would damage DNA, lipids, and proteins. These reactive oxygen species contributed to the lethality of the drug, a conclusion supported by the observed protective effects of an SpxB deletion. These results support the model whereby fluoroquinolones cause redox alterations. The transcriptional response of S. pneumoniae to moxifloxacin is compared with the response to levofloxacin, an inhibitor of topoisomerase IV. Levofloxacin triggers the transcriptional activation of iron transport genes and also enhances the Fenton reaction. PMID:26525786

  14. Plasma and Tissue Disposition of Moxifloxacin in Japanese Quail ( Coturnix japonica ).

    Science.gov (United States)

    Goudah, Ayman; Hasabelnaby, Sherifa

    2016-06-01

    Plasma disposition and depletion of moxifloxacin were investigated in Japanese quail ( Coturnix japonica ) after single intravenous, intramuscular, and oral administration of 5 mg/kg and after intramuscular and oral administration of 5 mg/kg q24h for 5 consecutive days, respectively. Drug concentrations in plasma and tissues were measured by high-performance liquid chromatography with fluorescence detection. After intravenous injection, plasma drug concentration-time curves were best described by a 2-compartment open model. The decline in plasma drug concentration was biexponential with half-lives of 0.3 hours and 2.18 hours for distribution and elimination phases, respectively. Steady-state volume of distribution and total body clearance after intravenous administration were estimated to be 1.12 L/kg and 0.41 L/h per kilogram, respectively. After intramuscular and oral administration of moxifloxacin at the same dose, the peak plasma concentrations were 2.14 and 1.94 μg/mL and were obtained at 1.4 and 1.87 hours, respectively, and the elimination half-lives were 2.56 and 1.97 hours, respectively. The systemic bioavailabilities were 92.48% and 87.94%, respectively. Tissue levels after intramuscular and oral administration were highest in liver and kidneys, respectively, and decreased in the following order: plasma, lungs, and muscle. Moxifloxacin concentrations after intramuscular and oral administration were below the detection limit of the assay in tissues and plasma after 120 hours. PMID:27315376

  15. Safety of prophylactic intracameral moxifloxacin ophthalmic solution after cataract surgery in patients with penetrating keratoplasty

    Institute of Scientific and Technical Information of China (English)

    Osman; Sevki; Arslan; Ceyhun; Arici; Mustafa; Unal; Erdogan; Cicik; Mehmet; Serhat; Mangan; Eray; Atalay

    2014-01-01

    AIM:To determine the safety of prophylactic intracameral moxifloxacin after cataract surgery in patients with penetrating keratoplasty(PKP).METHODS:In this retrospective study of consecutive patients who had phacoemulsification cataract surgery after PKP, were treated with intracameral moxifloxacin0.5% ophthalmic solution(0.5 mg/0.1 mL). The main outcome measures were anterior chamber reaction, best corrected visual acuity(BCVA), corneal endothelial cell count(ECC), and central corneal thickness(CCT).RESULTS:Fifty-five patients were recruited(26 males,29 females). The mean age was 54.36±4.97y(range 45-64y).All eyes had improved postoperative BCVA. The mean BCVA was 0.25 preoperatively and 0.57 postoperatively,which was statistically significant(P <0.001). One eye had 3+, 7 eyes had 2+, 12 eyes had 1+ and 8 eyes had trace amount of aqueous cells on the first day after surgery. All eyes had no anterior chamber cells at subsequent follow up examinations. Effective phacoemulsification time was 4.33 ±1.01 s. The mean ECC was 2340.20 cells/mm2 preoperatively and 1948.75 cells/mm21 mo postoperatively(P <0.001). The increase of21.09 μm in postoperative pachymetry 1mo after surgery was statistically significant(P <0.001).CONCLUSION:Nountowardeffectswereobservedafter intracameral injection of moxifloxacin(0.5 mg/0.1 mL) in terms of anterior chamber reaction, CCT, ECC, and visual rehabilitation at the conclusion of cataract surgery in patients with PKP.

  16. Effects of moxifloxacin exposure on the conjunctival flora and antibiotic resistance profile following repeated intravitreal injections

    Institute of Scientific and Technical Information of China (English)

    Mustafa; Atas; Burhan; Baskan; Ayse; zkse; Fatma; Mutlu; Sarιgüzel; Süleyman; Demircan; Emine; Pangal

    2014-01-01

    AIM:To evaluate the effects of moxifloxacin exposure on the conjunctival flora and antibiotic resistance profile following repeated intravitreal injections.METHODS:Seventy-two eyes of 36 patients [36 eyes in control group, 36 eyes in intravitreal injection(IVI) group]were enrolled in the study. All the eyes had at least one IVI and had diabetic macular edema(DME) or age-related macular degeneration(ARMD). Moxifloxacin was prescribed to all the patients four times a day for five days following injection. Conjunctival cultures were obtained from the lower fornix via standardized technique with every possible effort made to minimize contamination from the lids, lashes, or skin. Before the application of any ophthalmic medication, conjunctival cultures were obtained from both eyes using sterile cotton culture. An automated microbiology system was used to identify the growing bacteria and determine antibiotic sensitivity.RESULTS:The bacterial cultures were isolated from 72 eyes of 36 patients, sixteen of whom patients(44.4%)were male and twenty(55.6%) were female. Average age was 68.4 ±9.0(range 50-86). The average number of injections before taking cultures was 3.1+1.0. Forty-eight(66.7%) of 72 eyes had at least one significant organism.There was no bacterial growth in 8(20.5%) of IVI eyes and in 16(44.4%) of control eyes(P =0.03). Of the bacteria isolated from culture, 53.8% of coagulase negative staphylococci(CoNS) in IVI eyes and 47.2%CoNS in control eyes. This difference between IVI eyes and control eyes about bacteria isolated from culture was not statistically significant(P =0.2). Eleven of 25 bacteria(44.0%) isolated from IVI eyes and 11(57.9%) of 19 bacteria isolated from control eyes were resistant to oxacillin. The difference in frequency of moxifloxacine resistance between two groups was not statistically significant(12.0% in IVI eyes and 21.1% in control eyes)(P =0.44). There were no cases of resistance to vancomycin, teicoplanin and linezolid

  17. Efficacy of moxifloxacin & econazole against multidrug resistant (MDR Mycobacterium tuberculosis in murine model

    Directory of Open Access Journals (Sweden)

    U D Gupta

    2015-01-01

    Full Text Available Background & objectives: Studies have shown the bactericidal potential of econazole and clotrimazole against Mycobacterium tuberculosis under in vitro and ex vivo conditions along with their synergism with conventional antituberculosis drugs. These molecules were also found to be effective against different multidrug resistant (MDR M. tuberculosis isolates in vitro. Hence the present study was designed to evaluate the in vivo antimycobacterial potential of moxifloxacin and econazole alone and in combination against multidrug resistant tuberculosis (MDR-TB in a mice model. Methods: Mice were infected with 2.5×10 [7] bacilli of MDR strain of M. tuberculosis by aerosol route of infection. After four weeks of infection, chemotherapy was started orally by moxifloxacin 8.0 mg/kg body wt and econazole 3.3 mg/kg alone and in combination, as well as with four first line anti-tuberculosis drugs as a positive control. The animals were sacrificed and the lungs and spleen were excised under aspetic conditions. The tissues were homogenized with sterile normal saline, an aliquot of the homogenate was plated on Middlebrook 7H11 agar supplemented with oleate albumin dextrose catalase (OADC and incubated at 37°C for four weeks. The number of visible and individual colonies were counted. Results: The first line anti-tuberculosis drugs (RIF+INH+EMB+PZA after eight weeks of therapy had no impact as the bacillary load in lungs and spleens remained unchanged. However, econazole, moxifloxacin alone as well as in combination significantly reduced the bacillary load in lungs as well as in spleens of MDR-TB bacilli infected mice. Interpretation & conclusions: Co-administration of the two drugs (econazole and moxifloxacin to MDR-TB strain JAL-7782 infected mice exhibited additive effect, the efficacy of the drugs in combination being higher as compared with ECZ or MOX alone. These results were substantiated by histopathological studies. This study suggests the utility of

  18. Effect of burden and origin sites of premature ventricular contractions on left ventricular function by 7-day Holter monitor.

    Science.gov (United States)

    Xu, Wenhua; Li, Mingfang; Chen, Minglong; Yang, Bing; Wang, Daowu; Kong, Xiangqing; Chen, Hongwu; Ju, Weizhu; Gu, Kai; Cao, Kejiang; Liu, Hailei; Jiang, Qi; Shi, Jiaojiao; Cui, Yan; Wang, Hong

    2015-11-01

    Recent studies have shown that premature ventricular contractions (PVCs) could enlarge the heart, but its risk factors are incompletely understood as a single 24-hour recording cannot reflect the true PVC burden due to day-to-day variability. Our purpose was to investigate the effect of burden and origin sites on left ventricular (LV) function in patients with PVCs by 7-day Holter electrocardiography (ECG). From May 2012 to August 2013, 112 consecutive patients with PVCs were recruited from the authors' affiliated hospital. All patients received 2-dimensional transthoracic echocardiography, 12-lead routing ECG and 7-days Holter ECG. Serum N-terminal pro-brain natriuretic peptide (NT-proBNP) levels were measured. A total of 102 participants with PVCs were included in the final analysis. Origin of PVCs from the tricuspid annulus had the highest burden and NT-proBNP level. LV papillary muscle had a higher LV ejection fraction (EF) level and a lower LV end-systolic dimension (ESD) than other PVC foci (PHolter monitor showed the true PVC burden on patients with PVCs. PVCs with a lower burden or origin from the LV papillary muscle and the fascicle were relatively benign, while PVCs with a higher burden or origin from the tricuspid annulus may lead to cardiac dysfunction.

  19. Growth Recovery of Lemna gibba and Lemna minor Following a 7-Day Exposure to the Herbicide Diuron.

    Science.gov (United States)

    Burns, Mitchell; Hanson, Mark L; Prosser, Ryan S; Crossan, Angus N; Kennedy, Ivan R

    2015-08-01

    In agricultural catchments, aquatic ecosystems can experience a pulse exposure to pesticides. Following such exposure, non-target organisms that are not extirpated may recover. This paper investigates the potential of two duckweed species (Lemna minor and Lemna gibba) to recover from a 7-day exposure to different concentrations (0.4-208 µg L(-1)) of the herbicide diuron. There was significant inhibition in the growth and biomass after the initial 7-day exposure (e.g. frond number EC50=59.2 and 52.2 µg L(-1) for L. minor and L. gibba, respectively). Following transfer to clean media, recovery (the highest concentration yielding no significant difference in the effect endpoint from the control) was observed for all effects endpoints at concentrations ranging 60-111 µg L(-1) for L. minor and 60-208 µg L(-1) for L. gibba. These results suggest that recovery is possible for primary producers at environmentally relevant concentrations considered significant in ecological risk assessment. PMID:26067703

  20. Bactericidal activity of moxifloxacin against multidrug-resistant Streptoccocus pneumoniae at clinically achievable serum and epithelial lining fluid concentrations compared with three other antimicrobials.

    Science.gov (United States)

    Cafini, Fabio; Alou, Luis; Sevillano, David; Valero, Eva; Prieto, José

    2004-10-01

    Time-kill studies compared the activities of moxifloxacin with those of levofloxacin, azithromycin and amoxicillin-clavulanate against 10 pneumococcal strains with various drug susceptibilities. Three Streptococcus pneumoniae strains were resistant to moxifloxacin: 6, 7 and 2 strains were resistant to levofloxacin, azithromycin and amoxicillin-clavulanate, respectively. Of these, 1 strain was resistant to all antimicrobial agents studied. Moxifloxacin and amoxicillin-clavulanate were bactericidal after 24h at serum Cmax levels against 9 and 8 strains, respectively, while levofloxacin and azithromycin were bactericidal against 3 and 2 strains, respectively. A higher activity was only observed for amoxicillin-clavulanate for logarithmic phase cultures at 1, 4 and 8h compared with stationary phase organisms. Amoxicillin-clavulanate and moxifloxacin were bactericidal at free serum levels (protein unbound) after 24h against 8 and 3 strains, respectively. Moxifloxacin was bactericidal at epithelial lining fluid levels against all strains at 24h, including one moxifloxacin, amoxicillin-clavulanate and azithromycin-resistant strain; lower levels of bactericidal activity was observed for levofloxacin, azithromycin and amoxicillin-clavulanate against 7, 2 and 4 strains, respectively. This demonstrated the importance of moxifloxacin tissue levels. PMID:15380257

  1. Development and characterisation of thermo reversible mucoadhesive moxifloxacin hydrochloride in situ ophthalmic gel

    Directory of Open Access Journals (Sweden)

    M Dholakia

    2012-01-01

    Full Text Available A sustain release thermo reversible in situ gel of Moxifloxacin Hydrochloride using mucoadhesive polymer was prepared. Mucoadhesive polymer was used to obtain an ophthalmic delivery system with improved mechanical and mucoadhesive properties that will provide prolong retention time for treatment of ocular diseases. Developed formulations were evaluated for drug-excipient compatibility study, pH, Clarity, Gelation temperature study, Mucoadhesion properties and in-vitro release studies. Drug-excipient compatibility study was performed by FTIR technique.The individual IR spectra of the pure drug and polymers as well as the combination spectra of the drug and polymer were taken, which indicate no interaction between Moxifloxacin and polymers when compared with infrared spectrum of pure drug as all functional group frequencies were present. The values of other parameters obtained were in acceptable range. In vitro release tests revealed that 98% drug was released from the in situ gel containing 0.5% and 1.00% HPMC in 12 hr. provides prolonged release.

  2. Influence of Moxifloxacin on Hepatic Redox Status and Plasma Biomarkers of Hepatotoxicity and Nephrotoxicity in Rat

    Directory of Open Access Journals (Sweden)

    Ayokanmi Ore

    2015-01-01

    Full Text Available Moxifloxacin is a broad spectrum fluoroquinolone antibacterial agent. We examined the hepatic redox status and plasma biomarkers of nephrotoxicity and hepatotoxicity in rat following administration of moxifloxacin (MXF. Twenty-four Wistar rats, 180–200 g, were randomized into four groups (I–IV. Animals in group I (control received 1 mL of distilled water, while animals in groups II, III, and IV received 1 mL each of MXF equivalent to 4 mg/kg b.w., 8 mg/kg b.w., and 16 mg/kg b.w., respectively. After seven days, plasma urea, bilirubin, and creatinine were significantly (P<0.05 elevated in the MXF-treated animals. Activities of alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase were significantly increased in the plasma of MXF-treated animals compared to control. Also plasma total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglycerides increased significantly in the MXF-treated groups relative to control. Moreover, MXF triggered a significant decrease in hepatic catalase, superoxide dismutase, and glutathione-S transferase activities. Likewise, MXF caused a decrease in the hepatic levels of glutathione and vitamin C. A significant increase in hepatic MDA content was also observed in the MXF-treated animals relative to control. Overall, our data suggest that the half-therapeutic, therapeutic, and twice the therapeutic dose of MXF induced nephrotoxicity, hepatotoxicity, and altered hepatic redox balance in rats.

  3. Gender differences in endocrine responses to posture and 7 days of -6 degrees head-down bed rest

    Science.gov (United States)

    Vernikos, J.; Dallman, M. F.; Keil, L. C.; O'Hara, D.; Convertino, V. A.

    1993-01-01

    Endocrine regulation of fluids and electrolytes during 7 days of -6 degrees head-down bed rest (HDBR) was compared in male (n = 8) and, for the first time, female (n = 8) volunteers. The subjects' responses to quiet standing for 2 h before and after HDBR were also tested. In both sexes, diuresis and natriuresis were evident during the first 2-3 days of HDBR, resulting in a marked increase in the urinary Na(+)-to-K+ ratio and significant Na+ retention on re-ambulation. After the 1st day of HDBR, plasma renin activity (PRA) was increased relative to aldosterone (Aldo), plasma volume was decreased, and the renal response to Aldo appeared to be appropriate. Circulating levels of arginine vasopressin, cortisol, and ACTH were unchanged during HDBR. Plasma testosterone decreased slightly on day 2 of HDBR in males. The ratio of early morning ACTH to cortisol was lower in females than in males because ACTH was lower in females. Urinary cortisol increased and remained elevated throughout the HDBR in males only. There were no gender differences in the responses to 7 days of HDBR, except those in the pituitary-adrenal system; those differences appeared unrelated to the postural change. The provocative cardiovascular test of quiet standing before and after HDBR revealed both sex differences and effects of HDBR. There were significant sex differences in cardiovascular responses to standing before and after HDBR. Females had greater PRA and Aldo responses to standing before HDBR and larger Aldo responses to standing after HDBR than males.(ABSTRACT TRUNCATED AT 250 WORDS).

  4. In vitro permeation characteristics of moxifloxacin from oil drops through excised goat, sheep, buffalo and rabbit corneas.

    Science.gov (United States)

    Pawar, P K; Majumdar, D K

    2007-11-01

    The objective of present investigation was to study the in vitro permeation characteristics of moxifloxacin from oil drops through freshly excised goat, sheep, buffalo and rabbit corneas. Moxifloxacin, 0.043 to 0.048% (w/v) ophthalmic solutions with or without (0.5% v/v) benzyl alcohol were made in arachis, castor, cottonseed, olive, soybean, sunflower and sesame oils. Permeation studies were conducted by putting 1 ml oil formulation on cornea (0.50 cm2) fixed between donor and receptor compartments of an all glass modified Franz diffusion cell and measuring the drug permeated in receptor (containing 10 ml bicarbonate ringer, pH 7.4 at 37 degrees C under stirring) by spectrophotometry at 291 nm, after 120 min. Post permeation corneal hydration was measured to assess corneal damage. The study was designed with paired corneas i.e. one cornea of an animal received formulation without benzyl alcohol while the contralateral cornea received formulation with benzyl alcohol. Moxifloxacin ophthalmic solution in castor oil showed maximum permeation with all the corneas. Addition of benzyl alcohol, a preservative, to oil drops reduced permeation of moxifloxacin from each oil drop, with corneas of all the species. Partition experiments with moxifloxacin oil drops and phosphate buffer (pH 7.4) indicated higher partitioning of drug in the oil phase, in presence of benzyl alcohol. Thus results of permeation are consistent with the partition characteristics of drug between oil and aqueous phase. Corneal hydration obtained with all the formulations was between 75 to 80% indicating no corneal damage.

  5. Levofloxacin plus metronidazole administered once daily versus moxifloxacin monotherapy against a mixed infection of Escherichia coli and Bacteroides fragilis in an in vitro pharmacodynamic model.

    Science.gov (United States)

    Hermsen, Elizabeth D; Hovde, Laurie B; Sprandel, Kelly A; Rodvold, Keith A; Rotschafer, John C

    2005-02-01

    Moxifloxacin has been suggested as an option for monotherapy of intra-abdominal infections. Recent data support the use of a once-daily metronidazole regimen. The purpose of this study was to investigate the activity of levofloxacin (750 mg every 24 h [q24h]) plus metronidazole (1,500 mg q24h) compared with that of moxifloxacin (400 mg q24h) monotherapy in a mixed-infection model. By using an in vitro pharmacodynamic model in duplicate, Escherichia coli and Bacteroides fragilis were exposed to peak concentrations of 8.5 mg of levofloxacin/liter q24h, 32 mg of metronidazole/liter q24h, and 2 mg for moxifloxacin/liter q24h for 24 h. The activities of levofloxacin, metronidazole, moxifloxacin, and levofloxacin plus metronidazole were evaluated against E. coli, B. fragilis, and E. coli plus B. fragilis. The targeted half-lives of levofloxacin, metronidazole, and moxifloxacin were 8, 8, and 12 h, respectively. Time-kill curves were analyzed for time to 3-log killing, slope, and regrowth. Pre- and postexposure MICs were determined. The preexposure levofloxacin, metronidazole, and moxifloxacin MICs for E. coli and B. fragilis were 0.5 and 1, >64 and 0.5, and 1 and 0.25 mg/liter, respectively. Levofloxacin and moxifloxacin achieved a 3-log killing against E. coli and B. fragilis in all experiments, as did metronidazole against B. fragilis. Metronidazole did not decrease the starting inoculum of E. coli. The area under the concentration-time curve/MIC ratios for E. coli and B. fragilis were 171.7 and 85.9, respectively, for levofloxacin and 26 and 103.9, respectively, for moxifloxacin. Levofloxacin plus metronidazole exhibited the fastest rates of killing. The levofloxacin and moxifloxacin MICs for B. fragilis increased 8- to 16-fold after the organism was exposed to moxifloxacin. No other changes in the postexposure MICs were found. Levofloxacin plus metronidazole administered once daily exhibited activity similar to that of moxifloxacin against the mixed E. coli and B

  6. Activities of New Antimicrobial Agents (Trovafloxacin, Moxifloxacin, Sanfetrinem, and Quinupristin-Dalfopristin) against Bacteroides fragilis Group: Comparison with the Activities of 14 Other Agents

    OpenAIRE

    Betriu, Carmen; Gómez, María; Palau, M. Luisa; Sánchez, Ana; Picazo, Juan J.

    1999-01-01

    The antimicrobial activities of trovafloxacin, moxifloxacin, sanfetrinem, quinupristin-dalfopristin, and 14 other antimicrobial agents against 218 Bacteroides fragilis group strains were determined. A group of 10 imipenem-resistant strains were also tested. Imipenem, meropenem, and sanfetrinem had the lowest MICs of all of the β-lactams. Quinupristin-dalfopristin inhibited all of the strains at 2 μg/ml. Overall, the MICs of trovafloxacin and moxifloxacin for 90% of the strains tested were 1 a...

  7. In-vitro Wirksamkeit von Moxifloxacin und Linezolid gegen Staphylococcus aureus-, Streptococcus pneumoniae- und Enterococcus spp.-Isolate in Abhängigkeit vom Testmedium und der Keimlokalisation

    OpenAIRE

    Wilhelm, Cornelia

    2004-01-01

    Ziel der vorliegenden Arbeit ist die Untersuchung der zuvor nicht bekannten Beeinflussung der Aktivität von bestimmten Antibiotika durch unterschiedliche Testnährmedien. Zu diesem Zweck wird die Aktivität von Moxifloxacin, Linezolid, Penicillin G, Oxacillin und Cefuroxim gegen Bakterienisolate der Spezies S. aureus, S. pneumoniae, E. faecalis und E. faecium in Bouillon und Blut getestet. Des Weiteren wird die Aktivität von Moxifloxacin und Linezolid gegen intrazellulär in humanen Granulozyten...

  8. Serine-to-Asparagine Substitution in the GyrA Gene Leads to Quinolone Resistance in Moxifloxacin-Exposed Chlamydia pneumoniae

    OpenAIRE

    Rupp, Jan; Gebert, Andreas; Solbach, Werner; Maass, Matthias

    2005-01-01

    Quinolone resistance of Chlamydia pneumoniae has not been described previously. Serial subcultures of C. pneumoniae under increasing moxifloxacin concentrations (0.0125 to 6.4 mg/liter) resulted in a 256-fold MIC increase compared to moxifloxacin-naive strains. GyrA gene sequencing revealed a novel point mutation with a Ser→Asn substitution. Subcultures under rifalazil and macrolides did not alter the respective MICs.

  9. Efficacy and safety of single and double doses of ivermectin versus 7-day high dose albendazole for chronic strongyloidiasis.

    Directory of Open Access Journals (Sweden)

    Yupin Suputtamongkol

    Full Text Available BACKGROUND: Strongyloidiasis, caused by an intestinal helminth Strongyloides stercoralis, is common throughout the tropics. It remains an important health problem due to autoinfection, which may result in hyperinfection and disseminated infection in immunosuppressed patients, especially patients receiving chemotherapy or corticosteroid treatment. Ivermectin and albendazole are effective against strongyloidiasis. However, the efficacy and the most effective dosing regimen are to be determined. METHODS: A prospective, randomized, open study was conducted in which a 7-day course of oral albendazole 800 mg daily was compared with a single dose (200 microgram/kilogram body weight, or double doses, given 2 weeks apart, of ivermectin in Thai patients with chronic strongyloidiasis. Patients were followed-up with 2 weeks after initiation of treatment, then 1 month, 3 months, 6 months, 9 months, and 1 year after treatment. Combination of direct microscopic examination of fecal smear, formol-ether concentration method, and modified Koga agar plate culture were used to detect strongyloides larvae in two consecutive fecal samples in each follow-up visit. The primary endpoint was clearance of strongyloides larvae from feces after treatment and at one year follow-up. RESULTS: Ninety patients were included in the analysis (30, 31 and 29 patients in albendazole, single dose, and double doses ivermectin group, respectively. All except one patient in this study had at least one concomitant disease. Diabetes mellitus, systemic lupus erythrematosus, nephrotic syndrome, hematologic malignancy, solid tumor and human immunodeficiency virus infection were common concomitant diseases in these patients. The median (range duration of follow-up were 19 (2-76 weeks in albendazole group, 39 (2-74 weeks in single dose ivermectin group, and 26 (2-74 weeks in double doses ivermectin group. Parasitological cure rate were 63.3%, 96.8% and 93.1% in albendazole, single dose oral

  10. Eficacia de la moxifloxacina en infecciones odontogénicas Efficacy of moxifloxacin in odontogenic infections

    Directory of Open Access Journals (Sweden)

    C.M. Ardila Medina

    2009-08-01

    Full Text Available Las infecciones de la boca se caracterizan por ser polimicrobianas, endógenas, oportunistas, dinámicas y mixtas debido a que intervienen bacterias aerobias y especialmente anaerobias. La mayor parte de estas infecciones son odontogénicas, siendo las más frecuentes caries, periodontitis, absceso periapical, absceso periodontal, pericoronaritis, pulpitis, sinusitis, osteítis e infección de los espacios aponeuróticos. Las quinolonas presentan una alta penetración tisular y una buena absorción cuando se suministra después de dosis orales, pero solamente los compuestos desarrollados recientemente como la Moxifloxacina demuestran suficiente efectividad clínica y microbiológica. El objetivo de este artículo es presentar la eficacia in vitro e in vivo de la Moxifloxacina para el tratamiento de las infecciones de origen dental.The infections of the mouth are characterized for being polimicrobians, endogenous, opportunistic, dynamic and mixed because aerobic and specially anaerobic bacteria take part. Most of these infections are odontogenics, being the most frequent decays, periodontitis, periapical abscess, periodontal abscess, pericoronitis, pulpitis, sinusitis, osteitis and infection of the aponeurotic spaces. Quinolonas displays a high tissue penetration and a good absorption when it is provided after oral doses, but only the compounds developed recently as the Moxifloxacin demonstrates sufficient clinical and microbiological effectiveness. The aim of this article is to present the effectiveness in vitro and in vivo of the Moxifloxacin for the treatment of the infections of dental origin.

  11. Gender differences in endocrine responses to posture and 7 days of 6 deg head down bed rest

    Science.gov (United States)

    Vernikos, J.; Dallman, M. F.; Keil, L. C.; Ohara, D.; Convertino, V. A.

    1993-01-01

    Endocrine regulation of fluids and electrolytes during seven days of 6 deg head down bed rest (HDBR) was compared in male (n = 8) and, for the first time, female (n = 8) volunteers. The subjects' responses to quiet standing for 2 hr before and after HDBR were also tested. In both sexes, diuresis and natriuresis were evident during the first 2-3 days of HDBR, resulting in a marked increase in the urinary Na/K ratio and significant Na retention on reambulation. After the first day of HDBR, plasma renin activity (PRA) was increased relative to aldosterone, plasma volume was decreased, and the renal response to aldosterone appeared to be appropriate. Circulating levels of arginine vasopressin (AVP), cortisol, and ACTH were unchanged during HDBR. Plasma testosterone decreased slightly on day 2 of HDBR in males. The ratio of AM ACTH to cortisol was lower in females than in males because ACTH was lower in females. Urinary cortisol increased and remained elevated throughout the HDBR in males only. There were no gender differences in the responses to 7 day HDBR, except those in the pituitary-adrenal system; those differences appeared unrelated to the postural change. The provocative cardiovascular test of quiet standing before and after bed rest revealed both sex differences and effects of HDBR. There were significant sex differences in cardiovascular responses to standing, before and after HDBR. Females had greater PRA and aldosterone responses to standing before bedrest and larger aldosterone responses to standing after HDBR than males. Cardiovascular responses to standing before and after bedrest differed markedly: arterial pressure and heart rates increased with standing before HDBR, by contrast, arterial pressure decreased, with greater increases in heart rates after HDBR. In both sexes, all hormonal responses to standing were greater after HDBR. The results show clearly that similar responses to standing as well as to HDBR occur in both sexes, but that females exhibit

  12. Moxifloxacin Injection

    Science.gov (United States)

    ... appropriate for them to remain available as a therapeutic option.FDA is continuing to assess safety issues ... review has shown that fluoroquinolones when used systemically (i.e. tablets, capsules, and injectable) are associated with disabling ...

  13. Low Serum Concentrations of Moxifloxacin, Prothionamide, and Cycloserine on Sputum Conversion in Multi-Drug Resistant TB

    OpenAIRE

    Lee, Seung Heon; Seo, Kyung-Ah; Lee, Young Min; Lee, Hyun-Kyung; Kim, Je Hyeong; Shin, Chol; Ghim, Jong-Ryul; Shin, Jae-Gook; Kim, Dong hyun

    2015-01-01

    Purpose Low serum concentrations of drugs used to treat multi-drug resistant tuberculosis (MDR-TB) have occasionally been associated with treatment failure. We determined the frequencies of low serum concentrations of anti-MDR-TB drugs, and assessed the effects of these concentrations on 2-month sputum conversion. Materials and Methods The serum levels of moxifloxacin (MF), prothionamide (PTH), and cycloserine (CS) were determined for 89 serum samples by high-pressure liquid chromatography-ta...

  14. Comparative In Vitro Activities of Linezolid, Quinupristin-Dalfopristin, Moxifloxacin, and Trovafloxacin against Erythromycin-Susceptible and -Resistant Streptococci

    OpenAIRE

    Betriu, Carmen; Redondo, Montserrat; Palau, M. Luisa; Sánchez, Ana; Gómez, María; Culebras, Esther; Boloix, Ana; Picazo, Juan J.

    2000-01-01

    The in vitro activities of the new agents linezolid, quinupristin-dalfopristin, moxifloxacin, and trovafloxacin were determined and compared with those of penicillin, clindamycin, and four macrolides against 53 erythromycin-resistant Streptococcus pneumoniae, 117 S. pyogenes (64 erythromycin-susceptible and 53 -resistant), and 101 S. agalactiae (53 erythromycin-susceptible and 48 -resistant) isolates. Differentiation of macrolide resistance phenotypes was performed by the double-disk method. ...

  15. Clostridium difficile with Moxifloxacin/Clindamycin Resistance in Vegetables in Ohio, USA, and Prevalence Meta-Analysis

    OpenAIRE

    Alex Rodriguez-Palacios; Sanja Ilic; LeJeune, Jeffrey T.

    2014-01-01

    We (i) determined the prevalence of Clostridium difficile and their antimicrobial resistance to six antimicrobial classes, in a variety of fresh vegetables sold in retail in Ohio, USA, and (ii) conducted cumulative meta-analysis of reported prevalence in vegetables since the 1990s. Six antimicrobial classes were tested for their relevance as risk factors for C. difficile infections (CDIs) (clindamycin, moxifloxacin) or their clinical priority as exhaustive therapeutic options (metronidazole...

  16. Should Moxifloxacin Be Used for the Treatment of Extensively Drug-Resistant Tuberculosis? An Answer from a Murine Model▿

    OpenAIRE

    Poissy, Julien; Aubry, Alexandra; Fernandez, Christine; Lott, Marie-Catherine; Chauffour, Aurelie; Jarlier, Vincent; Farinotti, Robert; Veziris, Nicolas

    2010-01-01

    The prevalence of extensively drug-resistant tuberculosis (XDR-TB), defined as TB that is resistant to isoniazid, rifampin, fluoroquinolones, and aminoglycosides, is rising worldwide. The extent of Mycobacterium tuberculosis resistance to fluoroquinolones depends on the mutation in the DNA gyrase, the only target of fluoroquinolones. The MIC of moxifloxacin, the most active fluoroquinolone against M. tuberculosis, may be lower than its peak serum level for some ofloxacin-resistant strains of ...

  17. 莫西沙星引起QT间期异常延长%Abnormal QT interval prolongation associated with moxifloxacin

    Institute of Scientific and Technical Information of China (English)

    郑策; 甄健存

    2011-01-01

    One 87-year-old man with a history of atrial fibrillation and coronary heart disease for more than 20 years, was intravenously given 400 mg of moxifloxacin once daily for suspected lung infection. On the second day of moxifloxacin administration, the patient developed dyspnea and dizziness, and electrocardiogram showed prolongation of the QTc interval to 489 ms, which was 54 ms longer than the value at the time of admission (435 ms). Moxifloxacin was promptly withdrawn. Eight hours later, the QTc interval was 442 ms, which was close to the baseline range, and had no abnormal change during the therapy time.%1例87岁男性患者,房颤、冠心病史20余年,因怀疑肺部感染静脉用莫西沙星400 mg,qd.次日,患者出现胸闷、头晕,心电图显示QTc489ms,较入院时的435ms延长了54ms.立即停用莫西沙星,8h后,QTc442ms,接近入院时水平,以后均未有异常改变.

  18. 莫西沙星和左氧氟沙星致急性肾小管间质肾病%Acute interstitial nephritis caused by moxifloxacin and levofloxacin

    Institute of Scientific and Technical Information of China (English)

    李颖慧; 杭永付; 梁雁

    2016-01-01

    A 31-year-old male patient received an oral moxifloxacin 0. 4 g once daily for respiratory infection and after 3 days of treatment,his symptoms were not improved and appeared urine decrease. Moxifloxacin was stopped and changed into levofloxacin 0. 2 g twice daily by mouth. After 8 days of treatment,the patient's symptoms was not improved apparently and appeared urinary protein( + ),5-10 erythrocytes/ high magnification( HP),3-5 white blood cells/ HP,serum creatinine 419 μmol/ L. Renal biopsy showed acute tubular interstitial nephropathy. Drug-induced acute interstitial nephritis-induced by moxifloxacin and levofloxacin was considered. Levofloxacin was stopped and an IV infusion of methylpred-nisolone 250 mg once daily was given,and 3 days later,it was changed into oral prednison 40 mg once daily. After 7 days of treatment,the patient' s blood creatinine was 168 mol/ L,urea 13. 6 mmol/ L, glomerular filtration rate 45. 8 ml/ min/ 1. 73 m2 . The patient got better and was discharged. At 2 months of follow up,the patient's blood creatinine was 119 μmol/ L and urea was 6. 2 mmol/ L.%1例31岁男性患者因呼吸道感染给予莫西沙星0.4 g、1次/ d 口服,用药3 d 病情无改善并出现尿量减少;换用左氧氟沙星0.2 g、2次/ d 口服,用药8 d 病情仍无明显好转,且出现尿蛋白(+),5~10个红细胞/ HP,3~5个白细胞/ HP,Scr 419μmol/ L。肾活检示急性肾小管间质肾病。考虑可能为莫西沙星和左氧氟沙星引起的急性间质性肾炎。停用左氧氟沙星,给予甲泼尼龙250 mg、1次/ d 静脉滴注;3 d 后改为泼尼松40 mg、1次/ d 口服。治疗7 d 后患者 Scr 降至168μmol/ L,尿素13.6 mmol/ L,eGFR 45.8 ml·min-1·1.73 m-2。2个月后复查,患者 Scr 119μmol/ L,尿素6.2 mmol/ L。

  19. A stream-gaging network analysis for the 7-Day, 10-year annual low flow in New Hampshire streams

    Science.gov (United States)

    Flynn, Robert H.

    2003-01-01

    The 7-day, 10-year (7Q10) low-flow-frequency statistic is a widely used measure of surface-water availability in New Hampshire. Regression equations and basin-characteristic digital data sets were developed to help water-resource managers determine surface-water resources during periods of low flow in New Hampshire streams. These regression equations and data sets were developed to estimate streamflow statistics for the annual and seasonal low-flow-frequency, and period-of-record and seasonal period-of-record flow durations. generalized-least-squares (GLS) regression methods were used to develop the annual 7Q10 low-flow-frequency regression equation from 60 continuous-record stream-gaging stations in New Hampshire and in neighboring States. In the regression equation, the dependent variables were the annual 7Q10 flows at the 60 stream-gaging stations. The independent (or predictor) variables were objectively selected characteristics of the drainage basins that contribute flow to those stations. In contrast to ordinary-least-squares (OLS) regression analysis, GLS-developed estimating equations account for differences in length of record and spatial correlations among the flow-frequency statistics at the various stations. A total of 93 measurable drainage-basin characteristics were candidate independent variables. On the basis of several statistical parameters that were used to evaluate which combination of basin characteristics contribute the most to the predictive power of the equations, three drainage-basin characteristics were determined to be statistically significant predictors of the annual 7Q10: (1) total drainage area, (2) mean summer stream-gaging station precipitation from 1961 to 90, and (3) average mean annual basinwide temperature from 1961 to 1990. To evaluate the effectiveness of the stream-gaging network in providing regional streamflow data for the annual 7Q10, the computer program GLSNET (generalized-least-squares NETwork) was used to analyze the

  20. Levofloxacin plus Metronidazole Administered Once Daily versus Moxifloxacin Monotherapy against a Mixed Infection of Escherichia coli and Bacteroides fragilis in an In Vitro Pharmacodynamic Model

    OpenAIRE

    Hermsen, Elizabeth D.; Hovde, Laurie B.; Sprandel, Kelly A.; Rodvold, Keith A.; Rotschafer, John C.

    2005-01-01

    Moxifloxacin has been suggested as an option for monotherapy of intra-abdominal infections. Recent data support the use of a once-daily metronidazole regimen. The purpose of this study was to investigate the activity of levofloxacin (750 mg every 24 h [q24h]) plus metronidazole (1,500 mg q24h) compared with that of moxifloxacin (400 mg q24h) monotherapy in a mixed-infection model. By using an in vitro pharmacodynamic model in duplicate, Escherichia coli and Bacteroides fragilis were exposed t...

  1. Simultaneous determination of moxifloxacin and ofloxacin in physiological fluids using high performance liquid chromatography with ultraviolet detection.

    Science.gov (United States)

    Khan, Fahim Ullah; Nasir, Fazli; Iqbal, Zafar; Khan, Ismail; Shahbaz, Naila; Hassan, Muhammad; Ullah, Farhad

    2016-04-01

    A novel, sensitive and validated RP-HPLC-UV method was developed for simultaneous determination of moxifloxacin and ofloxacin using timolol maleate as internal standard in physiological fluids. Different experimental parameters were optimized and validated according to international guidelines. Complete separation of the analytes was achieved with Kromasil 100-5C18 analytical column (250mm×4.6mm×5μm), methanol and 0.05% trifloroacetic acid (TFA) (38:62v/v) were used as mobile phase, pumped at flow rate of 1.1ml/min in isocratic phase, column oven temperature maintained at 45°C and detection wavelength of 290nm. Protein precipitation method was applied to extract the drugs from human plasma and bovine aqueous humor samples using methanol as precipitating solvent. This method is linear in concentration range of 0.018-100μg/ml for moxifloxacin and 0.014-20μg/ml for ofloxacin. The recoveries of the method were 97.52 and 97.39% in human plasma for MX and OFN respectively, while in aqueous humor 94.48% for MX. The LOD values in plasma were found to be 10.0 and 8.00ng/ml for MX and OFN respectively, while their respective LOQ values were 18.0 and 14ng/ml. In aqueous humor the LOD and LOQ for MX were 16.0 and 24ng/ml respectively. In future, this method will be used to study the pharmacokinetic profile of moxifloxacin and ofloxacin in biological fluids and pharmaceutical products. PMID:26970846

  2. Kinetics of kill of bacterial conjunctivitis isolates with moxifloxacin, a fluoroquinolone, compared with the aminoglycosides tobramycin and gentamicin

    Directory of Open Access Journals (Sweden)

    Rudolph S Wagner

    2010-01-01

    Full Text Available Rudolph S Wagner1, David B Granet2, Steven J Lichtenstein3, Tiffany Jamison4, Joseph J Dajcs4, Robert D Gross5, Paul Cockrum41New Jersey Medical School, Newark, NJ, USA; 2Ratner Children’s Eye Center, University of California – San Diego, La Jolla, CA, USA; 3University of Illinois College of Medicine at Peoria, Peoria, Illinois, USA; 4Alcon Research, Ltd, Fort Worth, TX, USA; 5Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX, USAPurpose: To compare the kinetics and speed of kill of Streptococcus pneumoniae and Haemophilus influenzae on exposure to three topical ophthalmic antibiotic solutions.Materials and methods: Bacterial conjunctivitis isolates of S. pneumoniae and H. influenzae were exposed to 1:1000 dilutions of moxifloxacin 0.5%, tobramycin 0.3%, gentamicin 0.3%, and water (control. At 15, 30, 60, 120, and 180 minutes after exposure, aliquots were collected, cells were cultured, and viable cell counts were determined using standard microbiological methods.Results: Moxifloxacin achieved 99.9% kill (3-log reduction at approximately 2 hours for S. pneumoniae and at 15 minutes for H. influenzae. Tobramycin and gentamicin did not achieve 3-log reduction of S. pneumoniae during the 180-minute study period. An increase in bacterial growth was noted for these isolates. Gentamicin took more than 120 minutes to achieve the 3-log reduction of H. influenzae and tobramycin did not reach the 3-log reduction of this pathogen during the 180-minute study period.Conclusion: Moxifloxacin killed S. pneumoniae and H. influenzae in vitro faster than tobramycin and gentamicin, suggesting its potential clinical benefit as a first-line treatment for bacterial conjunctivitis to minimize patient symptoms and to limit the contagiousness of the disease.Keywords: kinetics of kill, bacterial conjunctivitis, in vitro, Streptococcus pneumoniae, Haemophilus influenzae, fluoroquinolones, aminoglycosides

  3. Effects of a mouthwash with chlorine dioxide on oral malodor and salivary bacteria: a randomized placebo-controlled 7-day trial

    Directory of Open Access Journals (Sweden)

    Ohnuki Mari

    2010-02-01

    Full Text Available Abstract Background Previous research has shown the oxidizing properties and microbiological efficacies of chlorine dioxide (ClO2. Its clinical efficacies on oral malodor have been evaluated and reported only in short duration trials, moreover, no clinical studies have investigated its microbiological efficacies on periodontal and malodorous bacteria. Thus, the aim of this study was to assess the inhibitory effects of a mouthwash containing ClO2 used for 7 days on morning oral malodor and on salivary periodontal and malodorous bacteria. Methods/Design A randomized, double blind, crossover, placebo-controlled trial was conducted among 15 healthy male volunteers, who were divided into 2 groups. Subjects were instructed to rinse with the experimental mouthwash containing ClO2 or the placebo mouthwash, without ClO2, twice per day for 7 days. After a one week washout period, each group then used the opposite mouthwash for 7 days. At baseline and after 7 days, oral malodor was evaluated with Organoleptic measurement (OM, and analyzed the concentrations of hydrogen sulfide (H2S, methyl mercaptan (CH3SH and dimethyl sulfide ((CH32S, the main VSCs of human oral malodor, were assessed by gas chromatography (GC. Clinical outcome variables included plaque and gingival indices, and tongue coating index. The samples of saliva were microbiologically investigated. Quantitative and qualitative analyses were performed using the polymerase chain reaction-Invader method. Results and Discussion The baseline oral condition in healthy subjects in the 2 groups did not differ significantly. After rinsing with the mouthwash containing ClO2 for 7 days, morning bad breath decreased as measured by the OM and reduced the concentrations of H2S, CH3SH and (CH32S measured by GC, were found. Moreover ClO2 mouthwash used over a 7-day period appeared effective in reducing plaque, tongue coating accumulation and the counts of Fusobacterium nucleatum in saliva. Future research is

  4. Characterisation of patients receiving moxifloxacin for acute bacterial rhinosinusitis in clinical practice: results from an international, observational cohort study.

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    Ralph Mösges

    Full Text Available We conducted a prospective, non-controlled, multi-centre Phase IV observational cohort study of patients with acute bacterial rhinosinusitis who were treated with moxifloxacin in clinical practice in 19 countries in Asia Pacific, Europe and the Middle East. With the data collected we evaluated the presentation and course of the current disease episode, particularly in terms of the principal clinical signs and symptoms of acute rhinosinusitis and diagnostic procedures. A final assessment of moxifloxacin therapy was made to evaluate the impact of the sinusitis episode on activities of daily life and on sleep disturbance, and to evaluate the clinical outcome of treatment. A total of 7,090 patients were enrolled, of whom 3909 (57.6% were included in the valid for clinical outcome and safety population. Regional differences were observed in the main symptoms of acute rhinosinusitis and, according to several characteristics, disease episodes appeared to be more severe in patients in Europe than in the Asia Pacific or Middle East regions. The sinusitis episode impacted on daily living for mean (SD periods of 3.6 (3.2, 4.6 (3.9 and 3.1 (3.0 days and disturbed sleep for 3.6 (3.2, 4.6 (3.9 and 3.1 (3.0 nights in the Asia Pacific, Europe and Middle East regions, respectively. With moxifloxacin treatment, the mean (SD time to improvement of symptoms was 3.0 (1.5, 3.4 (1.6 and 3.2 (1.5 days, and the time to resolution of symptoms was 4.8 (2.6 days, 5.7 (2.4 days and 5.5 (2.5 days, in the Asia Pacific, Europe and Middle East regions, respectively. In conclusion, acute rhinosinusitis remains a substantial health burden with significant impact on patients' quality of life, and there are differences between global regions in the clinical presentation, diagnosis and clinical course of disease episodes. Moxifloxacin was an effective and well-tolerated treatment option in the overall population.ClinicalTrials.gov Identifier: NCT00930488.

  5. Eradication of common pathogens at days 2, 3 and 4 of moxifloxacin therapy in patients with acute bacterial sinusitis

    Directory of Open Access Journals (Sweden)

    Benson Alice

    2006-04-01

    Full Text Available Abstract Background Acute bacterial sinusitis (ABS is a common infection in clinical practice. Data on time to bacteriologic eradication after antimicrobial therapy are lacking for most agents, but are necessary in order to optimize therapy. This was a prospective, single-arm, open-label, multicenter study to determine the time to bacteriologic eradication in ABS patients (maxillary sinusitis treated with moxifloxacin. Methods Adult patients with radiologically and clinically confirmed ABS received once-daily moxifloxacin 400 mg for 10 days. Middle meatus secretion sampling was performed using nasal endoscopy pre-therapy, and repeated on 3 consecutive days during treatment. Target enrollment was 30 bacteriologically evaluable patients (pre-therapy culture positive for Streptococcus pneumoniae, Haemophilus influenzae or Moraxella catarrhalis and evaluable cultures for at least Day 2 and Day 3 during therapy visits, including at least 10 each with S. pneumoniae or H. influenzae. Results Of 192 patients enrolled, 42 were bacteriologically evaluable, with 48 pathogens isolated. Moxifloxacin was started on Day 1. Baseline bacteria were eradicated in 35/42 (83.3% patients by day 2, 42/42 (100% patients by day 3, and 41/42 (97.6% patients by day 4. In terms of individual pathogens, 12/18 S. pneumoniae, 22/23 H. influenzae and 7/7 M. catarrhalis were eradicated by day 2 (total 41/48; 85.4%, and 18/18 S. pneumoniae and 23/23 H. influenzae were eradicated by day 3. On Day 4, S. pneumoniae was isolated from a patient who had negative cultures on Days 2 and 3. Thus, the Day 4 eradication rate was 47/48 (97.9%. Clinical success was achieved in 36/38 (94.7% patients at the test of cure visit. Conclusion In patients with ABS (maxillary sinusitis, moxifloxacin 400 mg once daily for 10 days resulted in eradication of baseline bacteria in 83.3% of patients by Day 2, 100% by Day 3 and 97.6% by Day 4.

  6. A comparative study of moxifloxacin and levofloxacin in treating AECOPD%莫西沙星与左氧氟沙星治疗AECOPD的对比研究

    Institute of Scientific and Technical Information of China (English)

    周凤丽; 黄静; 李洪涛; 张文先; 朱家馨; 张天托

    2011-01-01

    目的 观察莫西沙星注射液治疗慢性阻塞性肺疾病急性发作(AECOPD)的疗效、副作用和具体用药方法。方法 收集住院的AECOPD患者113例,随机分成两组,分别以莫西沙星和左氧氟沙星(对照组)治疗,比较两组治疗后的疗效、细菌清除率及对预后指标(FEV1、呼吸困难、6min步行距离)的改善率及药物副作用;同时将莫西沙星治疗组随机分为持续静脉滴注组和静滴加口服序贯治疗组,比较两组治疗后的疗效、细菌清除率及对预后指标(FEV1、呼吸困难、6min步行距离)的改善率。结果 莫西沙星治疗组的临床有效率(77.78%)、细菌清除率(81.48%)及对三项预后指标的改善率(82.54%、73.02%、69.84%)均高于左氧氟沙星组,而药物副作用发生率两组相当。莫西沙星持续治疗组与序贯治疗组比较,临床有效率、细菌清除率及对三项预后指标的改善率均无明显差异。结论 莫西沙星用于治疗AECOPD优于左氧氟沙星,且持续静滴给药治疗AECOPD与序贯给药比较无优势,从经济学角度,应推荐序贯给药治疗。%Objective To observe the curative effects and side effects and administration of moxifloxacin injection when used to treat acute explosion chronic obstructive pulmonary disease(AECOPD). Methods 113 hospitalized patients of AECOPD were collected and randomizedly divided into two groups and were treated with moxifloxacin or levofloxacin respectivly. The efficacy rates, bacteriums eradication rates and the side effects of two groups were compared. And the improvement rates of FEV1 or walking distance of 6min or dyspnea were also compared. Besides, the patients of AECOPD treated with moxifloxacin were randomizedly divided into two groups and treated with moxifloxacin injections continuously or moxifloxacin injections and tables sequently respetively. Similarly, the differents were compared between the two groups. Results The efficacy

  7. Treatment duration of febrile urinary tract infection (FUTIRST trial): a randomized placebo-controlled multicenter trial comparing short (7 days) antibiotic treatment with conventional treatment (14 days)

    OpenAIRE

    Kuijper Ed J; Ablij Hans C; Delfos Nathalie M; Wattel-Louis G Hanke; Koster Ted; Leyten Eliane MS; Elzevier Henk W; Assendelft Willem JJ; van't Wout Jan W; van Nieuwkoop Cees; Pander Jan; Blom Jeanet W; Spelt Ida C; van Dissel Jaap T

    2009-01-01

    Abstract Background Current guidelines on the management of urinary tract infection recommend treating febrile urinary tract infection or acute pyelonephritis with antimicrobials for at least 14 days. Few randomized trials showed the effectiveness of treatment durations of 5 to 7 days but this has only been studied in young previously healthy women. Methods/Design A randomized placebo-controlled double-blind multicenter non-inferiority trial in which 400 patients with community acquired febri...

  8. Nutrition: a key environmental dietary factor in clinical severity and cardio-metabolic risk in psoriatic male patients evaluated by 7-day food-frequency questionnaire

    OpenAIRE

    Barrea, Luigi; Macchia, Paolo Emidio; Tarantino, Giovanni; Di Somma, Carolina; Pane, Elena; Balato, Nicola; Napolitano, Maddalena; Colao, Annamaria; Savastano, Silvia

    2015-01-01

    Background Western dietary pattern is included among the environmental dietary factors involved in the pathogenesis of psoriasis. Nutritional data collection methods and gender differences might affect the association between diet and psoriasis. The 7-day food records is considered the “gold standard” of self-administered food frequency questionnaires. In this study, we evaluated the differences in the dietary intake, anthropometric measurements and cardio-metabolic risk profile in a group of...

  9. Comparing a 7-day diary vs. 24 h-recall for estimating fluid consumption in overweight and obese Mexican women

    OpenAIRE

    Hernández-Cordero, Sonia; López-Olmedo, Nancy; Rodríguez-Ramírez, Sonia; Barquera-Cervera, Simón; Rivera-Dommarco, Juan; Popkin, Barry

    2015-01-01

    Background High intake of sugar-sweetened beverages (SSB) is linked to increased weight, energy intake, and diabetes. Even though the increasing interest on beverages and water intake, there are few dietary tools carefully validated. The purpose of this paper is to compare a fluid intake 7-day diary against a 24-h recall questionnaire to estimate the fluid consumption in overweight and obese women participating in a randomized controlled trial in Mexico. Methods This cross-sectional study exp...

  10. Quadruple therapy with moxifloxacin and bismuth for first-line treatment of Helicobacter pylori

    Institute of Scientific and Technical Information of China (English)

    Antonio Francesco Ciccaglione; Luigina Cellini; Laurino Grossi; Leonardo Marzio

    2012-01-01

    AIM:To compare triple therapy vs quadruple therapy for 10 d as first-line treatment of Helicobacterpylori (H.pylori) infection.METHODS:Consecutive H.pylori positive patients never treated in the past for this infection were randomly treated with triple therapy of pantoprazole (PAN) 20 mg bid,amoxicillin (AMO) 1 g bid and moxifloxacin (MOX) 400 mg bid for 10 d (PAM) or with quadruple therapy of PAN 20 mg bid,AMO 1 g bid,MOX 400 mg bid and bismuth subcitrate 240 mg bid for 10 d (PAMB).All patients were found positive at 13 C-Urea breath test (UBT) performed within ten days prior to the start of the study.A successful outcome was confirmed with an UBT performed 8 wk after the end of treatment.x2 analysis was used for statistical comparison.Per protocol (PP) and intention-to-treat (ITT) values were also calculated.RESULTS:Fifty-seven patients were enrolled in the PAM group and 50 in the PAMB group.One patient in each group did not return for further assessment.Eradication was higher in the PAMB group (negative:46 and positive:3) vs the PAN group (negative:44 and positive:12).The H.pylori eradication rate was statistically significantly higher in the PAMB group vs the PAM group,both with the PP and ITT analyses (PP:PAMB 93.8%,PAM 78.5%,P < 0.02; ITT:PAMB 92%,PAM 77.1%,P <0.03).CONCLUSION:The addition of bismuth subcitrate can be considered a valuable adjuvant to triple therapy in those areas where H.pylori shows a high resistance to fluoroquinolones.

  11. Evaluation of Trimethoprim/Sulfamethoxazole (SXT), Minocycline, Tigecycline, Moxifloxacin, and Ceftazidime Alone and in Combinations for SXT-Susceptible and SXT-Resistant Stenotrophomonas maltophilia by In Vitro Time-Kill Experiments

    Science.gov (United States)

    Cai, Xuejiu; Zhao, Jin; Cui, Junchang

    2016-01-01

    Background The optimal therapy for infections caused by Stenotrophomonas maltophilia (S. maltophilia) has not yet been established. The objective of our study was to evaluate the efficacy of trimethoprim/sulfamethoxazole (SXT), minocycline, tigecycline, moxifloxacin, levofloxacin, ticarcillin-clavulanate, polymyxin E, chloramphenicol, and ceftazidime against clinical isolated S. maltophilia strains by susceptibility testing and carried out time-kill experiments in potential antimicrobials. Methods The agar dilution method was used to test susceptibility of nine candidate antimicrobials, and time-killing experiments were carried out to evaluate the efficacy of SXT, minocycline, tigecycline, moxifloxacin, levofloxacin, and ceftazidime both alone and in combinations at clinically relevant antimicrobial concentrations. Results The susceptibility to SXT, minocycline, tigecycline, moxifloxacin, levofloxacin, ticarcillin-clavulanate, chloramphenicol, polymyxin E, and ceftazidime were 93.8%, 95.0%, 83.8%, 80.0%, 76.3%, 76.3%, 37.5%, 22.5%, and 20.0% against 80 clinical consecutively isolated strains, respectively. Minocycline and tigecycline showed consistent active against 22 SXT-resistant strains. However, resistance rates were high in the remaining antimicrobial agents against SXT-resistant strains. In time-kill experiments, there were no synergisms in most drug combinations in time-kill experiments. SXT plus moxifloxacin displayed synergism when strains with low moxifloxacin MICs. Moxifloxacin plus Minocycline and moxifloxacin plus tigecycline displayed synergism in few strains. No antagonisms were found in these combinations. Overall, compared with single drug, the drug combinations demonstrated lower bacterial concentrations. Some combinations showed bactericidal activity. Conclusions In S. maltophilia infections, susceptibility testing suggests that minocycline and SXT may be considered first-line therapeutic choices while tigecycline, moxifloxacin, levofloxacin

  12. Development and validation of sensitive kinetic spectrophotometric method for the determination of moxifloxacin antibiotic in pure and commercial tablets

    Science.gov (United States)

    Ashour, Safwan; Bayram, Roula

    2015-04-01

    New, accurate, sensitive and reliable kinetic spectrophotometric method for the assay of moxifloxacin hydrochloride (MOXF) in pure form and pharmaceutical formulations has been developed. The method involves the oxidative coupling reaction of MOXF with 3-methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH) in the presence of Ce(IV) in an acidic medium to form colored product with lambda max at 623 and 660 nm. The reaction is followed spectrophotometrically by measuring the increase in absorbance at 623 nm as a function of time. The initial rate and fixed time methods were adopted for constructing the calibration curves. The linearity range was found to be 1.89-40.0 μg mL-1 for initial rate and fixed time methods. The limit of detection for initial rate and fixed time methods is 0.644 and 0.043 μg mL-1, respectively. Molar absorptivity for the method was found to be 0.89 × 104 L mol-1 cm-1. Statistical treatment of the experimental results indicates that the methods are precise and accurate. The proposed method has been applied successfully for the estimation of moxifloxacin hydrochloride in tablet dosage form with no interference from the excipients. The results are compared with the official method.

  13. 1 case Moxifloxacin-induced abnormal taste%莫西沙星致味觉异常1例

    Institute of Scientific and Technical Information of China (English)

    许世伟

    2013-01-01

    A 31-year-old man patient with upper respiratory tract infection was given moxifloxacin hydrochloride tablet 0.4g qd po. Two hours later, He felt bitter and abnormal taste, He felt bitter and abnormal taste;on the fourth day, on the fourth day, he took moxifloxacin for some reason and the same symptom appeared again. Finally he decided not to take any again. The adverse effect faded away. He turned to cefpodoxime proxetil tablets. No adverse effect occurred this time.%  1例31岁男性患者,因上呼吸道感染给予盐酸莫西沙星片口服0.4g/次,一日1次.2小时后出现口苦、味觉异常等变化,3天后消失;第4天患者自行再次服用莫西沙星片后又出现味觉异常表现,停用后改为头孢泊肟酯片后未出现味觉异常.

  14. 莫西沙星的严重不良反应及其防范%Severe Adverse Drug Reactions Induced by Moxifloxacin and Their Prevention

    Institute of Scientific and Technical Information of China (English)

    陈晨钟; 史道华

    2011-01-01

    目的:提示临床高度关注莫西沙星的不良反应.方法:检索我院不良反应监测系统,对其中莫西沙星产生的3例严重不良反应报告进行分析.结果:3例莫西沙星严重不良反应中,暴发性肝衰竭1例,经抢救无效死亡;过敏性休克2例,经抢救未产生严重后果.结论:临床应重视莫西沙星严重不良反应的危害性.%OBJECTIVE: To prompt the adverse drug reactions (ADR) of moxifloxacin should be paid high attention to clinic.METHODS: Retrieved from ADR reporting and monitoring system of our hospital, 3 serious ADR cases induced by moxifloxacin were analyzed. RESULTS: Among 3 serious ADR cases induced by moxifloxacin, one case developed fulminant hepatic failure leading to death after rescue, and other 2 patients with anaphylactic shock was recovered without severe consequence after rescue.CONCLUSION:Great importance should be attached to ADR induced by moxifloxacin in the clinic.

  15. Susceptibilities of Mycobacterium marinum to Gatifloxacin, Gemifloxacin, Levofloxacin, Linezolid, Moxifloxacin, Telithromycin, and Quinupristin-Dalfopristin (Synercid) Compared to Its Susceptibilities to Reference Macrolides and Quinolones

    OpenAIRE

    Bråbäck, Martin; Riesbeck, Kristian; Forsgren, Arne

    2002-01-01

    The susceptibility pattern of Mycobacterium marinum was determined. Quinupristin-dalfopristin and telithromycin were less active than clarithromycin. Linezolid showed good antimicrobial activity at clinically achievable concentrations. Gatifloxacin, levofloxacin, and moxifloxacin displayed activities similar to those of ciprofloxacin. Gemifloxacin was less active. The Etest method showed variable agreement with the reference method.

  16. A validated stability-indicating high performance liquid chromatographic method for moxifloxacin hydrochloride and ketorolac tromethamine eye drops and its application in pH dependent degradation kinetics

    Directory of Open Access Journals (Sweden)

    Jayant B Dave

    2013-01-01

    Full Text Available Background and Aim: A fixed dose combination of moxifloxacin hydrochloride and ketorolac tromethamine is used in ratio of 1:1 as eye drops for the treatment of the reduction of post operative inflammatory conditions of the eye. A simple, precise, and accurate High Performance Liquid Chromatographic (HPLC method was developed and validated for determination of moxifloxacin hydrochloride and ketorolac tromethamine in eye drops. Materials and Methods: Isocratic HPLC separation was achieved on a ACE C 18 column (C 18 (5 μm, 150 mm×4.6 mm, i.d. using the mobile phase 10 mM potassium di-hydrogen phosphate buffer pH 4.6-Acetonitrile (75:25 v/v at a flow rate of 1.0 mL/min. The detection was performed at 307 nm. Drugs were subjected to acid, alkali and neutral hydrolysis, oxidation and photo degradation. Moreover, the proposed HPLC method was utilized to investigate the pH dependent degradation kinetics of moxifloxacin hydrochloride and ketorolac tromethamine in buffer solutions at different pH values like 2.0, 6.8 and 9.0. Results and Conclusion: The retention time (t R of moxifloxacin hydrochloride and ketorolac tromethamine were 3.81±0.01 and 8.82±0.02 min, respectively. The method was linear in the concentration range of 2-20 μ/mL each for moxifloxacin hydrochloride and ketorolac tromethamine with a correlation coefficient of 0.9996 and 0.9999, respectively. The method was validated for linearity, precision, accuracy, robustness, specificity, limit of detection and limit of quantitation. The drugs could be effectively separated from different degradation products and hence the method can be used for stability analysis. Different kinetics parameters like apparent first-order rate constant, half-life and t 90 (time for 90% potency left were calculated.

  17. Moxifloxacin Improves Treatment Outcomes in Patients with Ofloxacin-Resistant Multidrug-Resistant Tuberculosis.

    Science.gov (United States)

    Chien, Jung-Yien; Chien, Shun-Tien; Chiu, Wei-Yih; Yu, Chong-Jen; Hsueh, Po-Ren

    2016-08-01

    It is unclear whether the use of moxifloxacin (MFX), a newer synthetic fluoroquinolone, results in better outcomes in patients with ofloxacin (OFX)-resistant multidrug-resistant tuberculosis (MDR-TB). During the period from April 2006 to December 2013, a total of 2,511 patients with culture-confirmed tuberculosis (TB) were treated at a TB referral hospital in southern Taiwan. Of the 2,511 patients, 325 (12.9%) had MDR-TB, and of those 325 patients, 81 (24.9%) had OFX-resistant MDR-TB and were included in the study. Among the 81 patients with OFX-resistant MDR-TB, 50 (61.7%) were successfully treated and 31 (38.3%) had unfavorable outcomes, including treatment failure (n = 25; 30.9%), loss to follow-up (n = 2; 2.5%), and death (n = 4; 4.9%). Patients treated with MFX had a significantly higher rate of treatment success (77.3% versus 43.2%; odds ratio [OR] = 4.46, 95% confidence interval [CI] = 1.710 to 11.646, P = 0.002) than patients not treated with MFX, especially among those infected with MFX-susceptible isolates (40.7%) or isolates with low-level resistance to MFX (28.4%). Multivariate logistic regression analysis showed that treatment with MFX (adjusted odds ratio = 6.54, 95% CI = 1.44 to 29.59, P = 0.015) was the only independent factor associated with treatment success. Mutation at codon 94 in the gyrA gene was the most frequent mutation (68.0%) associated with high-level MFX resistance. Multivariate Cox proportional hazards regression analysis showed that treatment with MFX was also an independent factor associated with early culture conversion (hazard ratio = 3.12, 95% CI = 1.48 to 6.54, P = 0.003). Our results show that a significant proportion of OFX-resistant MDR-TB isolates were susceptible or had low-level resistance to MFX, indicating that patients with OFX-resistant MDR-TB benefit from treatment with MFX. PMID:27216062

  18. Intracameral cefuroxime and moxifloxacin used as endophthalmitis prophylaxis after cataract surgery: systematic review of effectiveness and cost-effectiveness

    Directory of Open Access Journals (Sweden)

    Linertová R

    2014-08-01

    Full Text Available Renata Linertová,1,2 Rodrigo Abreu-González,3 Lidia García-Pérez,1,2 Marta Alonso-Plasencia,3 Luis Mateo Cordovés-Dorta,4 José Augusto Abreu-Reyes,4 Pedro Serrano-Aguilar2,5 1Fundación Canaria de Investigación y Salud (FUNCIS, Santa Cruz de Tenerife, Spain; 2Red de Investigación en Servicios Sanitarios en Enfermedades Crónicas (REDISSEC, Madrid, Spain; 3Ophthalmology Service, University Hospital Ntra Sra de La Candelaria, Santa Cruz de Tenerife, Spain; 4Ophthalmology Service, University Hospital of Canary Islands, La Laguna, Spain; 5HTA Unit, Canary Health Service, Santa Cruz de Tenerife, Spain Abstract: Postoperative endophthalmitis is one of the most serious potential complications of ocular lens surgery. Its incidence can be reduced by means of antibiotic prophylaxis. Although the prophylactic use of intracameral cefuroxime has been extended, other drugs, such as moxifloxacin, have arisen as alternatives. We performed a systematic literature review on the effectiveness and efficiency of intracameral cefuroxime and moxifloxacin for the prophylaxis of postoperative endophthalmitis after cataract surgery. Several bibliographic databases were searched up to October 2010 and were updated up to January 2013. Outcomes were the onset of endophthalmitis after surgery and the cost-effectiveness ratio of using both antibiotic prophylaxis alternatives. The following were included: a clinical trial reported in two papers, six observational studies, and an economic evaluation. All studies assessed cefuroxime compared with another antibiotic prophylaxis or no prophylaxis. The only randomized controlled trial performed by the European Society of Cataract and Refractive Surgery found that intracameral cefuroxime is significantly more effective than not using prophylaxis or the use of a topical antibiotic. The observational studies support these results. The economic evaluation compared different prophylaxis regimens and concluded that intracameral

  19. Al3+-MXFX体系的紫外特性及莫西沙星的测定%UV characteristic of Al3+-moxifloxacin and determination of moxifloxacin tablets

    Institute of Scientific and Technical Information of China (English)

    朱红梅; 席会平; 龚文琪

    2009-01-01

    目的:建立Al3+增敏,紫外分光光度法测定莫西沙星(Moxifloxacin,MXFX)的含量.方法:基于在pH=6.0的缓冲溶液中,铝(Ⅲ)对莫西沙星的吸光度有显著的增敏作用,建立了铝(Ⅲ)增敏,紫外分光光度法快速测定莫西沙星的新方法.结果:将该方法用于MXFX片剂的测定,样品平均回收率为97.70%,RSD=2.68%,检出限为0.01542μg/ml.结论:该方法简便、快捷、准确.

  20. Efficacy of 7-Day and 14-Day Triple Therapy Regimens for the Eradication of Helicobacter pylori: A Comparative Study in a Cohort of Romanian Patients

    Science.gov (United States)

    Arama, Stefan Sorin; Tiliscan, Catalin; Negoita, Cristina; Croitoru, Alexandru; Arama, Victoria; Mihai, Carmen Marina; Pop, Florinel

    2016-01-01

    Objective. This study compared the eradication rates of of Helicobacter pylori (HP) infection by a 7-day and 14-day anti-HP regimen. Materials and Methods. An open, randomized, prospective study was performed to evaluate the response to anti-HP treatment in adult HP-positive patients following a 7-day course (Regimen A) of a proton pump inhibitor in association with clarithromycin and amoxicillin compared to a 14-day course (Regimen B). Gastric biopsies were performed at baseline and two months after anti-HP treatment. Results. Seventy-eight patients aged 18–64 years (28 males, 50 females) diagnosed with HP infection were included. Fifty-two (66.7%) patients received Regimen B and 26 (33.3%) Regimen A. The overall eradication rate was 70.5%. Better treatment response (p duodenal lesions in both regimens. Younger patients ≤35 years had a better response to Regimen B. Better treatment response was seen in women, urban residents, and those with tertiary level of education in both groups. Conclusion. 14-day anti-HP regimen offered a significant better overall eradication of HP in study population. PMID:26858750

  1. Analysis of mutations in the gyrA and gyrB genes and their association with the resistance of Mycobacterium tuberculosis to levofloxacin, moxifloxacin and gatifloxacin.

    Science.gov (United States)

    Nosova, Elena Yu; Bukatina, Anastasia A; Isaeva, Yulia D; Makarova, Marina V; Galkina, Ksenia Yu; Moroz, Arkadyi M

    2013-01-01

    The purpose of the present study was to analyse mutations in the gyrA and gyrB genes of Mycobacterium tuberculosis and define the possible correlation between these mutations and resistance to levofloxacin (LVX), moxifloxacin (MFX) and gatifloxacin (GAT), based on their MICs. One hundred and forty-two M. tuberculosis clinical isolates were collected from pulmonary tuberculosis patients in the Moscow region. All M. tuberculosis strains were tested for drug susceptibility to rifampicin and isoniazid using the BACTEC MGIT 960 System and to ofloxacin (OFX) using the absolute concentration method on solid Lowenstein-Jensen slants. All in all, 68 strains were selected at random (38 strains were resistant and 30 were susceptible to OFX) for further analysis using the TB-BIOCHIP-2 test system and DNA sequence analysis. The MICs of LVX, MFX and GAT for selected strains were determined using the BACTEC MGIT 960 System. Mutations in the gyrA gene were observed in 36 out of 38 (94.7 %) OFX-resistant M. tuberculosis strains. Asn538Asp and Asp500His substitutions in the gyrB gene only were found in two (5.3 %) strains. Twenty-nine out of 30 OFX-sensitive M. tuberculosis strains had no mutations in either gene. One (3.3 %) OFX-sensitive M. tuberculosis strain carried an Arg485His substitution in gyrB. The results of our investigation showed that there is no clear correlation between the type of mutation in the genes gyrA and gyrB, and the MIC levels of LVX, MFX and GAT for resistant strains. Mutations in gyrA and Asn538Asp, and Asp500His substitutions in gyrB were associated with cross-resistance of M. tuberculosis to fluoroquinolones. The substitution Arg485His in gyrB does not confer resistance to LVX, MFX and GAT in M. tuberculosis.

  2. Radiosynthesis and biological evaluation of the {sup 99m}Tc-tricarbonyl moxifloxacin dithiocarbamate complex as a potential Staphylococcus aureus infection radiotracer

    Energy Technology Data Exchange (ETDEWEB)

    Shah, Syed Qaiser, E-mail: ssqaiser2002@yahoo.co [Nuclear Medicine Research Laboratory (NMRL), University of Peshawar, Peshawar, KPK (Pakistan); Khan, Muhammad Rafiullah [Phytopharmaceutical and Neutraceuticals Research Laboratory (PNRL), University of Peshawar, Peshawar, KPK (Pakistan)

    2011-04-15

    In the present investigation, radiosynthesis of the {sup 99m}Tc-tricarbonyl moxifloxacin dithiocarbamate complex ({sup 99m}Tc(CO){sub 3}-MXND) and its biological evaluation in male Wister rats (MWR) artificially infected with Staphylococcus aureus (S. aureus) was assessed. The {sup 99m}Tc(CO){sub 3}-MXND complex was radiochemically examined in terms of stability in saline and in serum and biologically its in-vitro binding with S. aureus and percent absorption in MWR models. Radiochemically the {sup 99m}Tc(CO){sub 3}-MXND complex showed more than 90% stability in saline up to 240 min and in serum 14.95% undesirable species was appeared within 16 h. In-vitro the {sup 99m}Tc(CO){sub 3}-MXND complex showed saturated binding with S. aureus. In MWR artificially infected with live S. aureus the complex showed about six fold higher uptakes in the infected muscle as compared to the normal muscle. However, insignificant change in the uptake of {sup 99m}Tc(CO){sub 3}-MXND complex in the infected and inflamed or normal muscle was observed in the MWR infected with heat killed S. aureus. The {sup 99m}Tc(CO){sub 3}-MXND complex disappeared from the circulatory system and appeared in the urinary system within 60-90 min followed by excretion through normal route of urinary system. Based on the elevated and stable radiochemical succumb in saline, serum, saturated in-vitro binding with S. aureus and higher accumulation in the target organ of the MWR, we recommend the {sup 99m}Tc(CO){sub 3}-MXND complex for radio-localization of the infection induced by S. aureus in human.

  3. Mesoporous Silica Nanoparticles with pH-Sensitive Nanovalves for Delivery of Moxifloxacin Provide Improved Treatment of Lethal Pneumonic Tularemia.

    Science.gov (United States)

    Li, Zilu; Clemens, Daniel L; Lee, Bai-Yu; Dillon, Barbara Jane; Horwitz, Marcus A; Zink, Jeffrey I

    2015-11-24

    We have optimized mesoporous silica nanoparticles (MSNs) functionalized with pH-sensitive nanovalves for the delivery of the broad spectrum fluoroquinolone moxifloxacin (MXF) and demonstrated its efficacy in treating Francisella tularensis infections both in vitro and in vivo. We compared two different nanovalve systems, positive and negative charge modifications of the mesopores, and different loading conditions-varying pH, cargo concentration, and duration of loading-and identified conditions that maximize both the uptake and release capacity of MXF by MSNs. We have demonstrated in macrophage cell culture that the MSN-MXF delivery platform is highly effective in killing F. tularensis in infected macrophages, and in a mouse model of lethal pneumonic tularemia, we have shown that the drug-loaded MSNs are much more effective in killing F. tularensis than an equivalent amount of free MXF.

  4. [b][/b]Relative efficiencies of the Burkard 7-Day, Rotorod and Burkard Personal samplers for Poaceae and Urticaceae pollen under field conditions

    Directory of Open Access Journals (Sweden)

    Robert Peel

    2014-11-01

    Full Text Available [b]Introduction[/b]. In aerobiological studies it is often necessary to compare concentration data recorded with different models of sampling instrument. Sampler efficiency typically varies from device to device, and depends on the target aerosol and local atmospheric conditions. To account for these differences inter-sampler correction factors may be applied, however for many pollen samplers and pollen taxa such correction factors do not exist and cannot be derived from existing published work. [b]Materials and methods.[/b] In this study, the relative efficiencies of the Burkard 7-Day Recording Volumetric Spore Trap, the Sampling Technologies Rotorod Model 20, and the Burkard Personal Volumetric Air Sampler were evaluated for Urticaceae and Poaceae pollen under field conditions. The influence of wind speed and relative humidity on these efficiency relationships was also assessed. Data for the two pollen taxa were collected during 2010 and 2011–2012, respectively. [b]Results[/b]. The three devices were found to record significantly different concentrations for both pollen taxa, with the exception of the 7-Day and Rotorod samplers for Poaceae pollen. Under the range of conditions present during the study, wind speed was found to only have a significant impact on inter-sampler relationships involving the vertically-orientated Burkard Personal sampler, while no interaction between relative efficiency and relative humidity was observed. [b]Conclusions[/b]. Data collected with the three models of sampler should only be compared once the appropriate correction has been made, with wind speed taken into account where appropriate.

  5. Continuous 7-Days-A-Week External Beam Irradiation in Locally Advanced Cervical Cancer: Final Results of the Phase I/II Study

    International Nuclear Information System (INIS)

    Purpose: To evaluate the feasibility and efficacy of definitive continuous 7-days-a-week pelvic irradiation without breaks between external beam radiotherapy and brachytherapy in locally advanced cervical cancer. Methods and Materials: Between November 1998 and December 1999, 30 patients with International Federation of Obstetrics and Gynecology Stage IIB or IIIB cervical cancer were included in a prospective Phase I/II study of continuous 7-days-a-week pelvic irradiation, to the total Manchester point B dose of 40.0–57.6 Gy. The first 13 patients (Group A) were given a daily tumor dose of 1.6 Gy, and the remaining 17 patients (Group B) were given 1.8 Gy. One or two immediate brachytherapy applications (point A dose 10–20 Gy, each) were performed in 28 cases. Results: Two patients did not complete the irradiation because of apparent early progression of disease during the irradiation. Eleven of the 28 evaluable patients (39%; 45% and 35% in Groups A and B, respectively) completed their treatment within the prescribed overall treatment time. Acute toxicity (including severe European Organisation for Research and Treatment of Cancer/Radiation Therapy Oncology Group Grade 3 and 4 effects in 40%) was experienced by 83% of patients and resulted in unplanned treatment interruptions in 40% of all patients (31% and 47% of patients in Groups A and B, respectively). Severe intestinal side effects occurred in 31% and 41% of Patients in Groups A and B, respectively (p = 0.71). The 5-year overall survival probability was 33%. Cancer recurrence occurred in 63% of patients: 20% inside and 57% outside the pelvis. Cumulative incidence of late severe bowel and urinary bladder toxicity at 24 months was 15%. Conclusion: Continuous irradiation in locally advanced cervical cancer is associated with a high incidence of severe acute toxicity, resulting in unplanned treatment interruptions. Late severe effects and survival after continuous radiotherapy do not substantially differ from

  6. Moxifloxacin, Ofloxacin, Sparfloxacin, and Ciprofloxacin against Mycobacterium tuberculosis: Evaluation of In Vitro and Pharmacodynamic Indices That Best Predict In Vivo Efficacy▿

    OpenAIRE

    Shandil, Radha K.; Jayaram, Ramesh; Kaur, Parvinder; Gaonkar, Sheshagiri; Suresh, B. L.; Mahesh, B. N.; Jayashree, R.; Nandi, Vrinda; Bharath, Sowmya; V. Balasubramanian

    2006-01-01

    Members of the fluoroquinolone class are being actively evaluated for inclusion in tuberculosis chemotherapy regimens, and we sought to determine the best in vitro and pharmacodynamic predictors of in vivo efficacy in mice. MICs for Mycobacterium tuberculosis H37Rv were 0.1 mg/liter (sparfloxacin [SPX]) and 0.5 mg/liter (moxifloxacin [MXF], ciprofloxacin [CIP], and ofloxacin [OFX]). The unbound fraction in the presence of murine serum was concentration dependent for MXF, OFX, SPX, and CIP. In...

  7. An assessment of the tolerability of moxifloxacin 0.5% compared to azithromycin 1.0% in DuraSite®

    OpenAIRE

    Granet, David; Lichtenstein, Steven J; Onofrey, Bruce; Katz, James A

    2007-01-01

    This subject-masked, randomized, active and placebo-controlled study compared subjects’ perceptions of two antibiotic ophthalmic drops. One hundred and twenty-five healthy volunteers received two of the following solutions: moxifloxacin 0.5% ophthalmic solution (Vigamox®, Alcon Laboratories, Inc., Ft Worth, TX, USA), azithromycin 1% in DuraSite® (AzaSite™, Inspire Pharmaceuticals, Inc., Durham, NC, USA), or Tears Naturale II® (Alcon Laboratories, Inc., Ft. Worth, TX, USA) in contralateral eye...

  8. Choice of baseline in a multiple-dose thorough QT study (TQTS – effect on analysis of moxifloxacin-induced QTc prolongation

    Directory of Open Access Journals (Sweden)

    B Tyl, S Azzam, E Reinbolt

    2009-12-01

    Full Text Available B Tyl1, S Azzam2, E Reinbolt2, N Blanco1, J Olbertz3, W Wheeler11MDS Pharma Services, Centralized Cardiac Services, Baillet-en-France, France; 2MDS Pharma Services, Early Clinical Research, Lincoln (Nebraska, USA; 3MDS Pharma Services, Early Clinical Research, Phoenix (Arizona, USABackground: The primary endpoint of a thorough QT study (TQTS is the change from baseline in QT corrected (QTc measured on electrocardiograms (ECG tracings. It has been suggested that during a crossover study, the time-matched or predose baseline could be recorded. The choice of method for baseline ECG collection may influence the results and the cost of the TQTS.Objective: The objective of our study was to compare the collection of a time-matched baseline before each period (TMEACH, an average of all the time-matched baseline (TMMEAN, a time-matched baseline before period 1 (TMP1 and a predose baseline (PDEACH on QT interval prolongation induced by moxifloxacin, in a 30 subjects, 5 arm cross-over TQTS.Results: Moxifloxacin induced a similar significant increase in QT corrected using Fridericia’s formula (QTc (lower limit of the confidence interval excluded 5 ms at all time-points between 0.5 hours and 12 hours with all baseline methods. TMEACH was associated with a lower within subject variability (SD 5.59 ms than TMMEAN and TMP1 (5.90 and 6.90 ms, respectively. PDEACH was associated with the highest variability (7.41 ms.Conclusion: The collection of ECG tracings during a full baseline day before each period was associated with the lowest variability. However, the two more cost effective designs (TMP1 and PDEACH were sufficient, in this small TQTS, to significantly detect moxifloxacin.Keywords: thorough QT study, QT correction, baseline, design, superimposed representative complex, moxifloxacin

  9. Mental disorders attributed to moxifloxacin in an elderly patient%莫西沙星致高龄患者精神异常

    Institute of Scientific and Technical Information of China (English)

    谭喜莹

    2011-01-01

    1例87岁女性患者,因胸闷、心慌加重伴喘息、咯痰给予莫西沙星0.4g、1次/d口服,1d后改为莫西沙星0.4 g、1次/d静脉滴注.第3天患者出现烦躁、谵语、神志模糊,应答不切题,夜间症状加重.停用莫西沙星,并给予地西泮肌内注射,2d后上述症状消失.%A 87-year-old female patient received moxifloxacin 0.4 g once daily orally for increasing chest tightness and palpitation with short of breath and expectoration. One day later, her regimen was changed to an Ⅳ infusion of moxifloxacin 0.4 g once daily. On day 3, the patient experienced agitation, delirium, confusion, and irrelevant speech. And her symptoms aggravated at that night. Moxifloxacin was discontinued and IM diazepam was given. Two days later, the above-mentioned symptoms subsided.

  10. Changes in messenger RNA of pancreatic enzymes and intestinal cholecystokinin after a 7-day bile-pancreatic juice diversion from the proximal small intestine in rats.

    Science.gov (United States)

    Hara, H; Ochi, Y; Kasai, T

    1997-06-01

    We have previously demonstrated the bile-pancreatic juice (BPJ)-independent stimulation of pancreatic enzyme secretion in chronic BPJ-diverted rats. Pancreatic and intestinal adaptation to 7-day BPJ diversion was next examined. Pancreatic enzyme mRNA and cholecystokinin mRNA in the jejunal mucosa were measured in rats with BPJ diverted into the ileum (PBD rats) in comparison with the figures for rats with BPJ returned to the duodenum (normal rats) or laparotomized (Intact) rats under well-nourished conditions. Amylase mRNA in the pancreas was lower and trypsinogen plus chymotrypsinogen mRNA was higher in the PBD rats than in the intact rats. The change in pancreatic mRNA was similar to that in the specific activities of the enzymes after a chronic BPJ diversion. This finding suggests that these pancreatic enzymes were regulated by the mRNA level. The portal concentration of cholecystokinin in the postabsorptive period (exogenously non-stimulated status) was 4-fold higher in the PBD group than in the normal and intact groups. Cholecystokinin mRNA in the jejunal mucosa of PBD rats was somewhat higher than that of intact rats. These results suggest that intestinal cholecystokinin was predominantly increased at the translational or later stage by chronic BPJ diversion.

  11. Effect of Broiler Breeder Age and Glutamine Supplementation on the Development of the Intestinal Mucosa of 7-Day-Old Chicks

    Directory of Open Access Journals (Sweden)

    A Maiorka

    2016-03-01

    Full Text Available ABSTRACT The aim of this study was to evaluate the possible effects of glutamine and broiler breeder age on the development of intestinal mucosa in broiler chicks during first week of age. For this purpose, 32 one-day-old broiler chicks were distributed according to a completely randomized experimental design in a 2 x 2 factorial arrangement. Treatments consisted of two broiler breeder ages (30 and 60 weeks and two dietary glutamine supplementation levels (0% and 1%. The morphological development of intestinal mucosa, particularly villus height and crypt depth, was evaluated. The results showed that the supplementation with 1% of glutamine influenced the development of villus height in the duodenum (p=0.009, jejunum (p = 0.006, and ileum (p = 0.001, as well as crypt depth in the jejunum (p = 0.037 of 7-day-old broilers. These results suggest that the presence of glutamine influenced the development of intestinal mucosa during the first week of age, when these tissues are highly influenced by dietary components, especially by trophic agents. The results show that broiler breeder age (30 or 60 weeks did not influence the evaluated parameters.

  12. 莫西沙星与左氧氟沙星治疗社区获得性肺炎的对比分析%Clinical analysis on efficacy of moxifloxacin and levofloxacin in treatment of community acquired pneumonia

    Institute of Scientific and Technical Information of China (English)

    宋雪梅

    2013-01-01

    Objective To explore the efficacy of moxifloxacin and levofloxacin in treatment of community acquired pneumonia. Methods The clinical data of patients with community acquired pneumonia were collected, and they were divided into moxifloxacin group ( 30 cases ) and levofloxacin group ( 30 cases ). Results The duration for disappearance of coughing, defervescent time, normalized time for blood routine examination , and time for X - ray lung shadow apparent absorption > 50% in patients of moxifloxacin group were lower than those of patients in levofloxacin group, the total effectiveness rate of clinical cure in patients of moxifloxacin group was higher than that of patients in levofloxacin group. The rate of bacterial clearance in patients of moxifloxacin group was better than that of patients in levofloxacin group, and their difference was statistically significant ( P 50%时间均低于左氧氟沙星组,莫西沙星组细菌清除率和临床治疗总有效率均明显优于左氧氟沙星组,差异均有统计学意义(P<0.05).结论 莫西沙星治疗社区获得性肺炎临床症状改善明显,效果优于左氧左氧氟沙星,值得临床推广应用.

  13. Intravenous moxifloxacin in routine hospital treatment of respiratory tract infections in China: results of a multicenter, noninterventional study

    Directory of Open Access Journals (Sweden)

    Chen R

    2011-04-01

    Full Text Available Rongchang Chen1, Wenjiang Ma2, Xuezhong Yu3, Xinmin Liu4, Jihong Zhu5, Hong Liang6, Xiaomei Wu7, Tao Guo81State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, China; 2Respiratory Department, The First Affiliated Hospital of Medical School of Zhejiang University, China; 3Emergency Department, Peking Union Medical College Hospital, China; 4Geriatric Department, Peking University First Hospital, China; 5Emergency Department, Peking University People's Hospital, China; 6Respiratory Department, Huadong Hospital Affiliated to Fudan University, China; 7Respiratory Department, The Second Affiliated Hospital of Harbin Medical University, China; 8Hematology Department, Wuhan Union Hospital, ChinaObjective: To investigate the effectiveness, safety, and tolerability of moxifloxacin (MXF (intravenous [IV] or sequential therapy [IV followed by oral] under daily treatment conditions in a large number of patients with respiratory tract infections.Design: Patients with a diagnosis of respiratory tract infection should be treated with MXF IV and/or tablets 400 mg once daily for a duration at the physician's discretion. For each patient, the physician documented data at an initial visit and at the end of therapy (EOT visit and/or, in the case of sequential therapy, an interim visit when the patient switched to oral treatment.Results: A total of 1953 patients treated with MXF were documented and were valid for an effectiveness and safety evaluation. An improvement was observed in 98.1% (n = 1911/1949 of patients treated with MXF. Recovery was documented in 89.9% (n = 1754/1951 of the patients. At the EOT visit, severity of infection was assessed to be "relieved" or at least "improved" in 96.5% (n = 1873/1940 of the patients. Physicians assessed overall effectiveness as "good" or "very good" in 93.3% (n = 1822/1953 of all patients. The physicians' overall tolerability rating was "very good" or "good" in 93.5% (n

  14. Effect of chick weight, geometric mean diameter and sodium level in prestarter diets (1 to 7 days on broiler perfomance up to 21 days of age

    Directory of Open Access Journals (Sweden)

    AML Ribeiro

    2004-12-01

    Full Text Available Seven hundred and twenty Ross 308 chicks were raised in a controlled environment room, distributed in a factorial design with 3 hatching chick weights (37, 40 and 44 g, 3 geometric mean diameter (GMD (0.561; 0.783 and 0.997 mm and 4 total sodium levels (Na (0.12; 0.24; 0.36 and 0.48% in the pre-starter diet (1 to 7 days. From 8 to 21 days (d one single basal diet was used for all treatments. The thirty seven-gram chicks had the smallest yolk sack weight at 4d, smallest body weight (BW and feed intake (FI at 7d and 21d, but the same feed conversion (FC than the other groups of hatching weight. Chicks receiving diet with intermediate GMD had the greatest BW and FI at 7d, but at 21d this effect was no longer seen. The diet with finest particle size resulted in birds with the smallest gizzard weight at 7d. The 0.12%Na diet was statistically different from the other Na levels, resulting in chicks at 7d with the worst FC and lowest body weights. At 21d, BW still was the lowest for this group of chicks. Birds with 0.48%Na produced more watery excreta and less dry matter in the carcass at 7d. Water consumption (C H2O was influenced linearly by chick weight up to 0.36%Na level. In the 0.48%Na level, 40 and 44 g chicks had similar C H2O, which was different from 37-g chicks. For best performance, Na values were set from 0.31 to 0.48%. The three studied factors influenced quality of pre-starter diet and consequently chick performance.

  15. Spectrofluorimetric study of the interaction between europium(III) and moxifloxacin in micellar solution and its analytical application

    Science.gov (United States)

    Kamruzzaman, Mohammad; Alam, Al-Mahmnur; Lee, Sang Hak; Ragupathy, Dhanusuraman; Kim, Young Ho; Park, Sang-Ryoul; Kim, Sung Hong

    2012-02-01

    A sensitive spectrofluorimetric method has been developed for the determination of moxifloxacin (MOX) using europium(III)-MOX complex as a fluorescence probe in the presence of an anionic surfactant, sodium dodecyl benzene sulfonate (SDBS). The fluorescence (FL) intensity of Eu 3+ was enhanced by complexation with MOX at 614 nm after excitation at 373 nm. The FL intensity of the Eu 3+-MOX complex was significantly intensified in the presence of SDBS. Under the optimum conditions, it was found that the enhanced FL intensity of the system showed a good linear relationship with the concentration of MOX over the range of 1.8 × 10 -11-7.3 × 10 -9 g mL -1 with a correlation coefficient of 0.9998. The limit of detection of MOX was found to be 2.8 × 10 -12 g mL -1 with relative standard deviation (RSD) of 1.25% for 5 replicate determination of 1.5 × 10 -8 g mL -1 MOX. The proposed method is simple, offers higher sensitivity with wide linear range and can be successfully applied to determine MOX in pharmaceutical and biological samples with good reproducibility. The luminescence mechanism is also discussed in detail with ultraviolet absorption spectra.

  16. Moxifloxacin versus Levofloxacin for Acute Exacerbation of Chronic Obstructive Pulmonary Diseases: A Systematic Review%莫西沙星与左氧氟沙星治疗慢性阻塞性肺疾病急性加重期疗效及安全性比较的系统评价

    Institute of Scientific and Technical Information of China (English)

    王小虎; 刘晓菊

    2012-01-01

    of meta-analysis showed that moxifloxacin group was significantly superior to levofloxacin group in the effective rate (OR=3.15, 95%CI 1.80 to 5.49, P<0.000 1). The bacterial clearance rate in moxifloxacin group was also higher than that in the levofloxacin group (OR=2.79, 95%CI 1.30 to 5.97, P=0.008). In addition, adverse effects of moxifloxacin group were less than levofloxacin (OR=0.48, 95%CI 0.24 to 0.98, P=0.04). Conclusion Based on current studies, moxifloxacin is superior to levofloxacin in improving effective rate and bacterial clearance rate, and in lowering side effects when treating AECOPD. Hence it is considerable to use moxifloxacin instead of levofloxacin in the treatment of AECOPD if necessary. Due to the limitation of both quantity and quality of included studies, this conclusion should be further confirmed with more high quality and large sample studies.

  17. A SWOT Analysis of the Development of 7 Days Inn%7天连锁酒店发展的SWOT分析

    Institute of Scientific and Technical Information of China (English)

    周兴; 彭保发; 吴云超; 黄佳

    2015-01-01

    since the luxury hotel is beyond the accept scope of most people ,the economic hotels are more welcomed by many people,although the present market situation is sound and the market prospects are broad, but along with the rapid development of economic hotels ,there are also a lot of problems.This paper is started from the current situation of economic hotels in our country and took the typical representative 7 Days Inn for example. it the pointed out rea-sons why the economic hotels are developing rapidly and the main problems existing in development with the contrasive analysis and comparative analysis,data analysis and other analysis methods Comprehensively,and proposed the development prospect and strategies in this industry, ,like to lay emphasis on theme culture,to carry out the differential management, to select the optimal franchisees ,to establish perfect management system and so on,hoping to provide reference for the development of ecomonic hotel in our country.%由于高档酒店超出了多数人的接受范围,使得经济型酒店受到了很多人的欢迎,虽然目前市场形势较好,市场前景广阔,但在经济型酒店发展迅速同时,也存在着不少的问题。文章就是从我国经济型酒店的现状入手,以极具代表性的7天连锁酒店为例。综合运用对比分析、数据分析等不同分析方法,指出了近几年我国经济型酒店迅猛发展的原因和发展过程中存在的主要问题,提出了这个行业未来的发展前景和发展战略,如重视主题文化、实行差异化管理、择优选择加盟店、建立完善的管理系统等等,希望对我国经济型酒店的发展提供参考价值。

  18. Activation of K{sup +} channels and Na{sup +}/K{sup +} ATPase prevents aortic endothelial dysfunction in 7-day lead-treated rats

    Energy Technology Data Exchange (ETDEWEB)

    Fiorim, Jonaina, E-mail: nanafiorim@hotmail.com [Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, ES (Brazil); Ribeiro Júnior, Rogério Faustino, E-mail: faustino43@oi.com.br [Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, ES (Brazil); Azevedo, Bruna Fernades, E-mail: brunafernandes.azevedo@gmail.com [Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, ES (Brazil); Simões, Maylla Ronacher, E-mail: yllars@hotmail.com [Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, ES (Brazil); Padilha, Alessandra Simão, E-mail: ale_spadilha@yahoo.com.br [Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, ES (Brazil); Stefanon, Ivanita, E-mail: ivanita@pq.cnpq.br [Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, ES (Brazil); Alonso, Maria Jesus, E-mail: mariajesus.alonso@urjc.es [Departamento de Ciencias de la Salud III, Universidad Rey Juan Carlos, Alcorcón (Spain); Salaices, Mercedes, E-mail: mercedes.salaices@uam.es [Departamento de Farmacología, Universidad Autónoma de Madrid, Instituto de Investigación Hospital Universitario La Paz (IdiPaz) (Spain); Vassallo, Dalton Valentim, E-mail: daltonv2@terra.com.br [Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, ES (Brazil)

    2012-07-01

    Seven day exposure to a low concentration of lead acetate increases nitric oxide bioavailability suggesting a putative role of K{sup +} channels affecting vascular reactivity. This could be an adaptive mechanism at the initial stages of toxicity from lead exposure due to oxidative stress. We evaluated whether lead alters the participation of K{sup +} channels and Na{sup +}/K{sup +}-ATPase (NKA) on vascular function. Wistar rats were treated with lead (1st dose 4 μg/100 g, subsequent doses 0.05 μg/100 g, im, 7 days) or vehicle. Lead treatment reduced the contractile response of aortic rings to phenylephrine (PHE) without changing the vasodilator response to acetylcholine (ACh) or sodium nitroprusside (SNP). Furthermore, this treatment increased basal O{sub 2}{sup −} production, and apocynin (0.3 μM), superoxide dismutase (150 U/mL) and catalase (1000 U/mL) reduced the response to PHE only in the treated group. Lead also increased aortic functional NKA activity evaluated by K{sup +}-induced relaxation curves. Ouabain (100 μM) plus L-NAME (100 μM), aminoguanidine (50 μM) or tetraethylammonium (TEA, 2 mM) reduced the K{sup +}-induced relaxation only in lead-treated rats. When aortic rings were precontracted with KCl (60 mM/L) or preincubated with TEA (2 mM), 4-aminopyridine (4-AP, 5 mM), iberiotoxin (IbTX, 30 nM), apamin (0.5 μM) or charybdotoxin (0.1 μM), the ACh-induced relaxation was more reduced in the lead-treated rats. Additionally, 4-AP and IbTX reduced the relaxation elicited by SNP more in the lead-treated rats. Results suggest that lead treatment promoted NKA and K{sup +} channels activation and these effects might contribute to the preservation of aortic endothelial function against oxidative stress. -- Highlights: ► Increased free radicals production ► Increased Na{sup +}/K{sup +} ATPase activity ► Promotes activation of the K{sup +} channels and reduced vascular reactivity ► These effects preserve endothelial function against oxidative

  19. Cytotoxic effects in 3T3-L1 mouse and WI-38 human fibroblasts following 72 hour and 7 day exposures to commercial silica nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Stępnik, Maciej, E-mail: mstep@imp.lodz.pl [Nofer Institute of Occupational Medicine, Łódź (Poland); Arkusz, Joanna; Smok-Pieniążek, Anna [Nofer Institute of Occupational Medicine, Łódź (Poland); Bratek-Skicki, Anna; Salvati, Anna; Lynch, Iseult; Dawson, Kenneth A. [Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4 (Ireland); Gromadzińska, Jolanta [Nofer Institute of Occupational Medicine, Łódź (Poland); De Jong, Wim H. [National Institute for Public Health and the Environment, Antonie van Leeuwenhoeklaan 9 NL‐3720, Bilthoven (Netherlands); Rydzyński, Konrad [Nofer Institute of Occupational Medicine, Łódź (Poland)

    2012-08-15

    The potential toxic effects in murine (3T3-L1) and human (WI-38) fibroblast cell lines of commercially available silica nanoparticles (NPs), Ludox CL (nominal size 21 nm) and CL-X (nominal size of 30 nm) were investigated with particular attention to the effect over long exposure times (the tests were run after 72 h exposure up to 7 days). These two formulations differed in physico-chemical properties and showed different stabilities in the cell culture medium used for the experiments. Ludox CL silica NPs were found to be cytotoxic only at the higher concentrations to the WI-38 cells (WST-1 and LDH assays) but not to the 3T3-L1 cells, whereas the Ludox CL-X silica NPs, which were less stable over the 72 h exposure, were cytotoxic to both cell lines in both assays. In the clonogenic assay both silica NPs induced a concentration dependent decrease in the surviving fraction of 3T3-L1 cells, with the Ludox CL-X silica NPs being more cytotoxic. Cell cycle analysis showed a trend indicating alterations in both cell lines at different phases with both silica NPs tested. Buthionine sulfoximine (γ-glutamylcysteine synthetase inhibitor) combined with Ludox CL-X was found to induce a strong decrease in 3T3-L1 cell viability which was not observed for the WI-38 cell line. This study clearly indicates that longer exposure studies may give important insights on the impact of nanomaterials on cells. However, and especially when investigating nanoparticle effects after such long exposure, it is fundamental to include a detailed physico-chemical characterization of the nanoparticles and their dispersions over the time scale of the experiment, in order to be able to interpret eventual impacts on cells. -- Highlights: ► Ludox CL silica NPs are cytotoxic to WI-38 fibroblasts but not to 3T3-L1 fibroblasts. ► Ludox CL-X silica NPs are cytotoxic to both cell lines. ► In clonogenic assay both silica NPs induce cytotoxicity, higher for CL-X silica. ► Cell cycle analysis shows

  20. 莫西沙星引起不良反应及分析%Adverse reaction and analyse the causes of Moxifloxacin

    Institute of Scientific and Technical Information of China (English)

    罗耀群

    2013-01-01

    Objective To summarize analysis of adverse reactions caused by moxifloxacin, to guide the rational use of clinical. Methods The domestic literature in recent years, the reported adverse reactions of moxifloxacin, summary, analysis and summary. Results The main adverse reactions of gastrointestinal system, central nervous system, cardiovascular system, etc;The elderly are adverse reactions of high-risk groups;Dosage has no significant gender differences, injected more than the oral medicine. Conclusion Moxifloxacin is the fourth generation of new fluoroquinolone antibiotic, by a large number of applications in recent years, adverse reaction is increasingly outstanding, should strengthen the management and reasonable use.%目的总结分析莫西沙星引起的不良反应,以指导临床合理使用。方法查阅近年来国内文献,对报道莫西沙星的不良反应,进行总结、分析、汇总。结果不良反应主要表现为胃肠道系统、心血管系统等;老年人是发生不良反应的高危人群;无明显的的性别差异,给药途径为注射给药多于口服给药。结论莫西沙星是第四代新型氟喹诺酮类抗菌药物,近年来被大量的应用,不良反应日益突出,应加强管理合理使用。

  1. Separation and identification of moxifloxacin impurities in drug substance by high-performance liquid chromatography coupled with ultraviolet detection and Fourier transform ion cyclotron resonance mass spectrometry

    Institute of Scientific and Technical Information of China (English)

    Cai Sheng Wu; Zhi Xin Jia; Bao Ming Ning; Jin Lan Zhang; Song Wu

    2012-01-01

    In this paper,a high-performance liquid chromatography coupled with ultraviolet detection and Fourier transform-ion cyclotron resonance mass spectrometry (HPLC-UV/FTICRMS) method was described for the investigation of impurity profile in moxifloxacin (MOX) drug substance and chemical reference substance.Ten impurities were detected by HPLC-UV,while eight impurities were identified by using the high accurate molecular mass combined with multiple-stage mass spectrometric data and fragmentation rules.In addition,to our knowledge,five impurities were founded for the first time in MOX drug substance.

  2. Comparison of the IKr blockers moxifloxacin, dofetilide and E-4031 in five screening models of pro-arrhythmia reveals lack of specificity of isolated cardiomyocytes

    DEFF Research Database (Denmark)

    Nalos, L; Varkevisser, R; Jonsson, Mkb;

    2012-01-01

    Background and purpose Drug discovery and development require testing of new chemical entities for possible adverse effects. For cardiac safety screening, improved assays are urgently needed and isolated adult cardiomyocytes (CM) and human embryonic stem cell-derived cardiomyocytes (hESC-CM) may...... obtained from the normal rabbit. Arrhythmic outcome was defined as Torsade de Pointes (TdP) in the animal models, and early afterdepolarizations (EADs) in the cell models. Key results At clinically relevant concentrations (5-12 µM), moxifloxacin was free of pro-arrhythmic properties in all assays...

  3. A cross-over study comparing an online versus a paper 7-day food record: focus on total water intake data and participant’s perception of the records

    OpenAIRE

    Monnerie, B.; Tavoularis, L. G.; Guelinckx, I.; Hebel, P.; Boisvieux, T.; Cousin, A.; Le Bellego, L

    2015-01-01

    Purpose To compare (1) fluid, food and nutrient intake obtained with a paper versus an online version of a 7-day food record and (2) user’s acceptability of both versions of the food record. Methods A cross-over study was carried out in 2010 in France. A total of 246 participants aged 18–60 years reported their food and fluid intake using both versions of the 7-day food record, separated by a 7- to 14-day washout period. To help participants in estimating consumed portions, both versions of t...

  4. Redox-Triggered Release of Moxifloxacin from Mesoporous Silica Nanoparticles Functionalized with Disulfide Snap-Tops Enhances Efficacy Against Pneumonic Tularemia in Mice.

    Science.gov (United States)

    Lee, Bai-Yu; Li, Zilu; Clemens, Daniel L; Dillon, Barbara Jane; Hwang, Angela A; Zink, Jeffrey I; Horwitz, Marcus A

    2016-07-01

    Effective and rapid treatment of tularemia is needed to reduce morbidity and mortality of this potentially fatal infectious disease. The etiologic agent, Francisella tularensis, is a facultative intracellular bacterial pathogen which infects and multiplies to high numbers in macrophages. Nanotherapeutics are particularly promising for treatment of infectious diseases caused by intracellular pathogens, whose primary host cells are macrophages, because nanoparticles preferentially target and are avidly internalized by macrophages. A mesoporous silica nanoparticle (MSN) has been developed functionalized with disulfide snap-tops that has high drug loading and selectively releases drug intracellularly in response to the redox potential. These nanoparticles, when loaded with Hoechst fluorescent dye, release their cargo exclusively intracellularly and stain the nuclei of macrophages. The MSNs loaded with moxifloxacin kill F. tularensis in macrophages in a dose-dependent fashion. In a mouse model of lethal pneumonic tularemia, MSNs loaded with moxifloxacin prevent weight loss, illness, and death, markedly reduce the burden of F. tularensis in the lung, liver, and spleen, and are significantly more efficacious than an equivalent amount of free drug. An important proof-of-principle for the potential therapeutic use of a novel nanoparticle drug delivery platform for the treatment of infectious diseases is provided. PMID:27246117

  5. Synthesis and Structure Characterization of Moxifloxacin Hydrochloride%盐酸莫西沙星的合成及结构解析

    Institute of Scientific and Technical Information of China (English)

    沈小莉; 陈家润

    2015-01-01

    以莫西沙星母核为起始原料,经硼酸酯活化、烃化、酸解三步反应合成了盐酸莫西沙星,通过高分辨质谱(HRMS)、紫外光谱(UV)、红外光谱(IR)、核磁共振氢谱(1 HNMR)、核磁共振碳谱(13 CNMR)、DEPT 谱、氢-氢相关谱(H-H COSY)、NOESY 谱、HSQC 谱、二级高分辨质谱及 XRD 谱确证了盐酸莫西沙星的结构。%Using ethyl 1-cyclopropyl-6,7-difluoro-8-methoxy-4-oxo-1,4-dihydroquinoline-3-carboxylate as starting material,moxifloxacin hydrochloride was synthesized via a three-step reaction including borate ester ac-tivation,N-alkylation and acidification.The molecular structure of moxifloxacin hydrochloride was characterized by HRMS,UV,IR,1 HNMR,13 CNMR,DEPT,H-H COSY,NOESY,HSQC,two-stage HRMS and XRD.

  6. A randomized trial of the efficacy and safety of sequential intravenous/oral moxifloxacin monotherapy versus intravenous piperacillin/tazobactam followed by oral amoxicillin/clavulanate for complicated skin and skin structure infections

    NARCIS (Netherlands)

    Gyssens, I.C.J.; Dryden, M.; Kujath, P.; Nathwani, D.; Schaper, N.; Hampel, B.; Reimnitz, P.; Alder, J.; Arvis, P.

    2011-01-01

    OBJECTIVES: The primary aim of the RELIEF study was to evaluate the efficacy and safety of two sequential intravenous (iv)/oral regimens: moxifloxacin iv/oral versus piperacillin/tazobactam (TZP) iv followed by oral amoxicillin/clavulanate (AMC). PATIENTS AND METHODS: The study had a prospective, ra

  7. Activity of Moxifloxacin against Resistant Staphylococcus Biofilms in Vitro%莫西沙星对耐药葡萄球菌生物膜体外药效学研究

    Institute of Scientific and Technical Information of China (English)

    姜剑伟; 陈向东; 汪辉; 李魁; 范璐; 王素霞

    2015-01-01

    Objective: To investigate the antibacterial effect of moxifloxacin against four strains of clinical resistant Staphylococcus biofilms in vitro. Methods: MIC (minimal inhibitory concentrations), MBIC (minimal biofilm inhibitory concentration) and MBEC (minimal biofilm eradication concentration) were examined by microdilution in broth; the percentages of biofilms formed and the viable cells of biofilms were investigated; and the synergy effects against biofilms were evaluated by a microdilution checkerboard method. Results: Moxifloxacin can eradicat biofilms at the concentration of 16-256 mg·L-1; The biofilms formed significantly decreased when moxifloxacin was at 2×MIC; moxifloxacin at 100×MIC caused significant decrease in viable cells of biofilms; the fractional biofilm inhibitory concentrations (FBICs) of moxifloxacin in combination with topical antibiotic retapamulin were all less than 1.0. Conclusions: Moxifloxacin can inhibit and eradicate clinical resistant Staphylococcus biofilms in vitro; synergy was demonstrated for moxifloxacin in combination with topical antibiotic retapamulin against them.%目的:研究莫西沙星对4株临床耐药葡萄球菌生物膜的体外药效学。方法:采用微量肉汤稀释法测定莫西沙星的最低抑菌浓度(minimal inhibitory concentration,MIC)、最低抑制生物膜浓度(minimal biofilm inhibitory concentration,MBIC)和最低摧毁生物膜浓度(minimal biofilm eradication concentration,MBEC);测定莫西沙星对细菌生物膜形成量以及存活菌的影响;采用微量稀释棋盘法测定莫西沙星与局部用药瑞他帕林的联合抗生物膜效果。结果:莫西沙星在16~256 mg·L-1的范围内可完全摧毁细菌生物膜;2×MIC显著降低生物膜的形成量;100× MIC可显著降低生物膜存活菌数;与瑞他帕林的联合抗生物膜分数(fractional biofilm inhibitory concentration,FBIC)均小于1.0。结论:莫西沙星对4株耐药葡

  8. 93例莫西沙星致严重不良反应报告分析%Analysis of 93 Cases of Severe Adverse Drug Reactions Caused by Moxifloxacin

    Institute of Scientific and Technical Information of China (English)

    周冰; 张俊; 张黎明

    2011-01-01

    目的 分析93例莫西沙星致严重不良反应报告,探讨莫西沙星致严重不良反应的特点和规律,为临床安全合理用药提供参考.方法 对2004年1月1日至2009年12月31日,北京市药品不良反应监测中心收到的93例莫西沙星致严重不良反应报告按人群、药品、不良反应情况等方面进行统计、分析.结果 临床使用莫西沙星多数能够按照药品使用说明书使用,少数存在不合理用药现象.结论 使用莫西沙星应严格按药品使用说明书用药,加强用药过程监护,减少药品带来的伤害.%Objective To probe into the general pattern and characteristics of severs ADRs indused by moxifloxacin so as to procide references for clinic retional use of moxifloxacin. Methods A total of 93 cases indused by moxifloxacin received from 2004 to 2009 in Beijing were analyzed statistically. Results The clinical use of moxifloxacin most can defer to the drugs instruction, the minority exists the irreasonabl phenomenon. Conclusion The use of moxifloxacin should strictly accord to the drugs instruction, enhance medication monitoring process, reduce the injury which the drugs bring.

  9. Spectroscopic studies, thermal analyses and biological evaluation of new V(IV), Zr(IV) and U(VI) moxifloxacin complexes

    Science.gov (United States)

    Sadeek, Sadeek A.; El-Shwiniy, Walaa H.; Zordok, Wael A.; Kotb, Essam

    2011-12-01

    The synthesis and characterization of the new solid complexes [VO(MOX) 2H 2O]SO 4·11H 2O, [ZrO(MOX) 2Cl]Cl·15H 2O and [UO 2(MOX) 3](NO 3) 2·3H 2O formed in the interaction of moxifloxacin (MOX) with VOSO 4·H 2O, ZrOCl 2·8H 2O and UO 2(NO 3) 2·6H 2O in methanol and acetone as a solvents at room temperature were reported. The isolated solid complexes have been characterized with melting points, elemental analysis, molar conductance, magnetic moments studies, spectral (UV-Visible, IR and 1HNMR) as well as thermal analyses (TGA and DTG). The results support the formation of the complexes and indicate that moxifloxacin reacts as a bidentate ligand chelate to the metal ion through the pyridone oxygen and one carboxylato oxygen. The kinetic parameters of thermogravimetric (TGA) and its differential (DTG), such as activation energies, E*, enthalpies, Δ H*, entropies, Δ S* and Gibbs free energies, Δ G*, have been evaluated by using Coats-Redfern (CR) and Horowitz-Metzeger (HM) methods. The proposed structure of the ligand and their complexes were detected by using the density functional theory (DFT) at the B3LYP/CEP-31G level of theory. The bond stretching force constant and length of the U dbnd O for the [UO 2(MOX) 3](NO 3) 2·3H 2O complex were calculated. The antibacterial activity of the free moxifloxacin ligand and their metal complexes have been tested against some selected bacterial strains such as: Streptococcus aureus K1, Bacillus subtilis K22, Brevibacterium otitidis K76, Escherichia coli K32, Pseudomonas aeruginosa SW1 and Klebsiella oxytoca K42. The complexes showed good antibacterial effect to the selected bacterial strains as compared to the free ligand and Zr(IV) complex is very highly significant compared with the other two complexes.

  10. Randomized clinical trial of thrice-weekly 4-month moxifloxacin or gatifloxacin containing regimens in the treatment of new sputum positive pulmonary tuberculosis patients.

    Directory of Open Access Journals (Sweden)

    Mohideen S Jawahar

    Full Text Available BACKGROUND: Shortening tuberculosis (TB treatment duration is a research priority. This paper presents data from a prematurely terminated randomized clinical trial, of 4-month moxifloxacin or gatifloxacin regimens, in South India. METHODS: Newly diagnosed, sputum-positive HIV-negative pulmonary TB patients were randomly allocated to receive gatifloxacin or moxifloxacin, along with isoniazid and rifampicin for 4 months with pyrazinamide for first 2 months (G or M or isoniazid and rifampicin for 6 months with ethambutol and pyrazinamide for first 2 months (C. All regimens were administered thrice-weekly. Clinical and bacteriological assessments were done monthly during treatment and for 24 months post-treatment. The Data and Safety Monitoring Board recommended termination of the trial due to high TB recurrence rates in the G and M regimens. RESULTS: Of 416 patients in intent-to-treat analysis, 6 (5% of 124, 2 (2% of 110 and 2 (2% of 137 patients with drug-susceptible TB in the G, M and C arms respectively had unfavorable response at the end of treatment; during the next 24 months, 17 (15% of 115, 11 (11% of 104 and 8 (6% of 132 patients respectively, had TB recurrence. Of 38 drug-resistant patients 1 of 8 and 3 of 26 in the G and C arms respectively had unfavourable response at the end of treatment; and TB recurrence occurred in 2 of 7 and 2 of 23 patients, respectively. The differences in TB recurrence rates between the G and C arms was statistically significant (p = 0.02. Gastro-intestinal symptoms occurred in 23%, 22% and 9% of patients in the G, M and C arms respectively, but most reactions were mild and manageable with symptomatic measures; 1% required regimen modification. CONCLUSIONS: 4-month thrice-weekly regimens of gatifloxacin or moxifloxacin with isoniazid, rifampicin and pyrazinamide, were inferior to standard 6-month treatment, in patients with newly diagnosed sputum positive pulmonary TB. TRIAL REGISTRATION: Clinical Trials Registry

  11. 莫西沙星治疗慢性盆腔炎临床探讨%Clinical Observation of Moxifloxacin Treatment of Chronic Pelvic Inflammatory Disease

    Institute of Scientific and Technical Information of China (English)

    程金阳

    2015-01-01

    Objective To Study of moxifloxacin in the treatmentof chronic pelvic inflammatory disease treatment effect. MethodWe selected 320 patients with chronic pelvic inflammatory disease in 2013 June --2014 yearinJune I treated patients in Department of gynecology and obstetrics, Were randomly divided into 2 groups with 160 cases in each, The control group was given penicillin combined with metronidazole in treatment, The observation group was given moxifloxacin treatment,Comparative analysis of therapeutic effect and adverse reaction oftwo groups.ResultThe observation group, the total efficiency is 96.3%, The control group the total effective rate was 81.8%, The observation group was significantly better than the control group(P< 0.05);The observation group the incidence of adverse reaction was 2.5%;the controlgroup, the incidence rate of adverse reaction was 10.6%, There was significant difference between two groups (P<0.05).Conclusion Moxifloxacin treatment using can obviously improve the clinical symptoms of patients with chronic pelvic inflammatory disease,And less adverse reaction, It is worth to popularize in the clinical application.%目的:探讨莫西沙星治疗慢性盆腔炎的治疗效果。方法选取2013年6月—2014年6月该妇产科收治的慢性盆腔炎患者320例,随机分为2组各160例,对照组给予青霉素联合甲硝唑治疗,观察组给予莫西沙星治疗,对比分析两组治疗效果和不良反应。结果观察组总有效率为96.3%,对照组总有效率为81.8%,观察组明显优于对照组(P<0.05);观察组不良反应发生率为2.5%;对照组不良反应发生率为10.6%,两组比较差异有统计学意义(P<0.05)。结论慢性盆腔炎患者采用莫西沙星治疗可明显改善患者临床症状,且不良反应少,值得在临床推广应用。

  12. Comparing of moxifloxacin and azythromycin against urogenital chlamydia trachomatis%莫西沙星与阿奇霉素治疗泌尿生殖道沙眼衣原体感染疗效比较

    Institute of Scientific and Technical Information of China (English)

    展小飞; 王树椿; 陈昭; 刘全忠

    2012-01-01

    目的:比较莫西沙星与阿奇霉素两种抗生素治疗泌尿生殖道沙眼衣原体感染的疗效.方法:收集1 671例确诊为泌尿生殖道沙眼衣原体感染并服用莫西沙星或阿奇霉素治疗的患者的临床资料,对两种药物的治愈率进行统计分析.结果:(1)两种药物的病原学治愈率分别是:莫西沙星为83.61%,阿奇霉素为73.28%,但两种抗生素病原学治愈率均有逐年递减的趋势(均P>0.05).(2)临床学治愈率分别是:莫西沙星为74.88%,阿奇霉素为88.91%.结论:莫西沙星的病原学治愈率高于阿奇霉素,而阿奇霉素的临床学治愈率好于莫西沙星.%Objective: To compare the effects of moxifloxacin and azythromycin in treating with urogenital chlamydia trachomatis. Methods: The data of 1 671 patients who were insured to have got urogenital chlamydia trachomatis infection and treated with moxifloxacin or azythromycin were collected and the effects of the two antibiotics were analyzed with descriptive epidemiological method. Results: The etiology cure rate of moxifloxacin was 83.61%, that of azythromycin was 73.28%. What's more, their effects decreased year by year. The clinical cure rate of moxifloxacin was 74.88%, that of azythromycin was 88.91%. Conclusion: The etiology cure rate of moxifloxacin is higher than that of azythromycin, but the clinical cure rate of moxifloxacin is lower than that of azythromycin.

  13. Comparative Activities of Clinafloxacin, Grepafloxacin, Levofloxacin, Moxifloxacin, Ofloxacin, Sparfloxacin, and Trovafloxacin and Nonquinolones Linozelid, Quinupristin-Dalfopristin, Gentamicin, and Vancomycin against Clinical Isolates of Ciprofloxacin-Resistant and -Susceptible Staphylococcus aureus Strains

    OpenAIRE

    Jones, Mark E.; Visser, Maarten R.; Klootwijk, Miriam; Heisig, Peter; Verhoef, Jan; Schmitz, Franz-Josef

    1999-01-01

    The activities of eight fluoroquinolones and linezolid, quinupristin-dalfopristin (Synercid), gentamicin, and vancomycin were tested against 96 ciprofloxacin-susceptible and 205 ciprofloxacin-resistant Staphylococcus aureus strains. Overall, clinafloxacin, followed by moxifloxacin and trovafloxacin, was the most active quinolone tested. For all isolates, linezolid and quinupristin-dalfopristin showed activities that were at least comparable to vancomycin, with no cross-resistance to any other...

  14. Moxifloxacin hydrochloride treatment of respiratory tract infection of 44 cases%盐酸莫西沙星治疗下呼吸道感染44例临床分析

    Institute of Scientific and Technical Information of China (English)

    梁福贵

    2009-01-01

    目的 评价盐酸莫西沙星治疗下呼吸道感染的疗效.方法 88例下呼吸道感染患者随机分为两组,治疗组用盐酸莫西沙星注射液0.4g/次,qd;对照组用左氧氟沙星治疗.疗程均为7~10d.结果 莫西沙星组和左氧氟沙星组临床总有效率分别为93.2%和90.9%,细菌清除率为94.7%和89.5%,两组不良反应少见而轻微.结论 盐酸莫西沙星治疗下呼吸道感染疗效确切,可作为治疗下呼吸道感染的一线用药.%Objective To evaluate the moxifloxacin hydrochloride treatment of respiratory tract infection.Methods 88 cases of respiratory tract infections were, randomly divided into 2 groups, group therapy with moxifloxacin hydrochloride injection 0.4g / times, qd; with the control group. Courses are 7 ~ 10d. Results Moxifloxacin and levofloxacin clinical total effective rate of 93.2 percent and 90.9 percent; bacterial clearance rate 94.7% and 89.5%, two rare and minor side effects. Conclusion Moxifloxacin hydrochloride in the treatment of respiratory tract infection the exact effect can be used as the treatment of lower respiratory tract infection of the first-line drugs.

  15. Clinical Observation of Moxifloxacin in the Treatment of Cerebrovascular Disease Complicating with Aspiration Pneumonia%莫西沙星治疗脑血管病伴吸人性肺炎的临床观察

    Institute of Scientific and Technical Information of China (English)

    刘石磊; 焦雪; 杜娟; 陆思静

    2011-01-01

    OBJECTIVE: To evaluate the efficacy and safety of moxifloxacin in the treatment of cerebrovascular disease complicating with aspiration pneumonia.METHODS: 112 patients with cerebrovascular disease complicating with aspiration pneumonia who were treated with moxifloxacin(0.4 g, qd, iv.gtt) were enrolled in this study.The clinical efficacy and safety were analyzed.RESULTS: The effective rate and curative rate of moxifloxacin therapy were 86 % and 55 %, respectively.The bacterial eradication rate was 77%, and the rate of adverse drug reaction was 7%.CONCLUSION: Moxifloxacin therapy is effective in the treatment of cerebrovascular disease complicating with aspiration pneumonia with few adverse drug reaction.%目的:观察莫西沙星治疗脑血管病伴吸入性肺炎患者的有效性和安全性.方法:选择112例脑血管病伴吸入性肺炎患者,应用莫西沙星(0.4 g,qd,静脉滴注)治疗,观察其临床疗效、安全性.结果:莫西沙星治疗脑血管病患者吸入性肺炎有效率为86%,痊愈率为55%,细菌清除率为77%,不良反应发生率为7%.结论:莫西沙星治疗脑血管病伴吸入性肺炎效果好,不良反应少.

  16. Clinical Efficacy of Moxifloxacin in the Treatment of Anaerobic Infection%莫西沙星治疗厌氧菌感染的临床疗效观察

    Institute of Scientific and Technical Information of China (English)

    陈琦; 何双军; 陈怡; 朱长清

    2011-01-01

    Objective:To evaluate the clinical efficacy of moxifloxacin in the treatment of anaerobic infection.Methods:The clinical efficacy for anaerobic bacteria infection was analyzed in a randomized,cotrolled clinical trial of moxifloxacin compared with metronidazole therapy in the treatment of 250 cases.Results:The efficacy rate was 90.3% in moxifloxacin group and 79.2% in the control gruop.The incidence of drug-resistant strains in the two gruops was 0% and 2.2%,respectively.The difference between groups was statistically significant(P<0.05).Conclusion:The use of moxifloxacin in the treatment of anaerobic infection revealed increased efficacy and lower incidence of drug-resistant strains.%目的:评价莫西沙星治疗厌氧菌感染的临床疗效.方法:在莫西沙星与甲硝唑治疗厌氧菌感染的临床随机对照研究中,对250例厌氧菌感染患者的疗效作对照分析.结果:莫西沙星组和对照组有效率分别为90.3%和79.2%,耐药菌株发生率分别为0%和2.2%,两组比较差异有统计学意义(P<0.05).结论:莫西沙星在治疗厌氧菌感染中有显著疗效且较少产生耐药菌株.

  17. 莫西沙星与左氧氟沙星治疗社区获得性肺炎的效果分析%The Clinical Analyze of Curing Community Acquired Pneumonia by Moxifloxacin and Levofloxacin

    Institute of Scientific and Technical Information of China (English)

    刘锋涛; 张玮

    2013-01-01

      目的:探讨莫西沙星与左氧氟沙星治疗社区获得性肺炎(CAP)的效果情况。方法:分析我院收治的社区获得性肺炎患者临床资料,依据治疗方式不同分为莫西沙星组40例和左氧氟沙星组40例。结果:莫西沙星组咳嗽消失时间、体温恢复正常时间、血常规恢复时间、胸部阴影明显吸收>50%时间均低于左氧氟沙星组,莫西沙星组临床治疗总有效率明显高于左氧氟沙星组,P50%time of moxifloxacin group were lower than levofloxacin group,the clinical curing total efficiency of moxifloxacin group were higher than levofloxacin group,The pulmonary function and quality of life of detection group were better than control group,P<0.05,the difference were statistical significance. Conclusion:The clinical symptoms of curing community acquired pneumonia by moxifloxacin was obviously and effect of good,which was to be used.

  18. Comparison of moxifloxacin hydrochloride with azithromycin in treatment of female genital infection%莫西沙星与阿奇霉素对比治疗女性生殖道支原体感染疗效观察

    Institute of Scientific and Technical Information of China (English)

    郭丽娜

    2011-01-01

    目的:对比观察盐酸莫西沙星及阿奇霉素治疗女性生殖道支原体感染的疗效.方法:78例患者随机分为2组:40例口服盐酸莫西沙星400mg每日一次;38例口服阿奇霉素500mg每日一次,疗程6d,结果:两组患者疗效观察,临床总有效率分别为90%、76.32%.结论:盐酸莫西沙星是治疗女性生殖道支原体感染有效、安全的新抗菌药物.%Objective: To compare the efficacy of moxifloxacin hydrochloride and azithromyc in treatment of female genital infection Methods A total of 78 patients were randomized into 2 groups: 40 cases of oral moxifloxacin hydrochloride 400mg once a day: 38 cases of oral azithromycin 500mg once daily for 6 days Results:The clinical total effective rate was 90% and 7632% in the 2 grnups, respectively Conclusion: Moxifloxacin hydrochloride is a safe and effective drug for treatment of female genital mycoplasma infection

  19. Sterility test method for moxifloxacin hydrochloride and sodium chloride injection%盐酸莫西沙星氯化钠注射液无菌检查方法的建立

    Institute of Scientific and Technical Information of China (English)

    孙红岩; 于风平

    2014-01-01

    Objective To establish a sterility test method for moxifloxacin hydrochloride and sodium chloride injection. Methods A test method was set up and improved on the basis of the officia line of Chinese Pharmacopoeia(edited in 2010). Results Antibacterial activity of moxifloxacin hydrochloride could be eliminated by membrane filtration method in which a water solution of 0.1% peptone was used as rinse solution. The volume of the rinse solution was 400 ml, 0.1 mol/L of manganese sulfate solution was used for neutralization. Conclusion The method established in the paper is valid in sterility test for moxifloxacin hydrochloride and sodium chloride injection.%目的:建立盐酸莫西沙星氯化钠注射液无菌检查方法。方法参照《中国药典》2010年版方法进行试验。结果采用薄膜过滤法(冲洗量:400 ml/膜)并结合中和法(每100 ml培养基加入:0.1 mol/L的硫酸锰溶液5 ml)即可消除样品对各菌株的抗菌活性。结论建立的方法可行,可用于盐酸莫西沙星氯化钠注射液无菌检查。

  20. 钙黄绿素-刚果红荧光光度法测定片剂及尿液中莫西沙星含量%Fluorometric determination of moxifloxacin with calcein-congo red

    Institute of Scientific and Technical Information of China (English)

    蔡花真; 席会平; 郭海星

    2011-01-01

    It was found that the effective energy transfer could occur between Congo Red(CR) and Calcein in the Britton-Robinson solution ( pH6. 00), which quenched the fluorescence intensity of Calcein. The fluorescence of Calcein reappeared with the addition of moxifloxacin. Therefore, a novel fluorescence method was developed for indirect determination of moxifloxacin. This method has been applied to the determination of moxifloxacin in tablets and urine, and the analytical results were in agreement with those obtained by the HPLC method.%在pH6.0的Britton-Robinson(B-R)缓冲溶液中,刚果红与钙黄绿素能够发生有效的能量转移,使钙黄绿素荧光猝灭.莫西沙星的加入,又使得钙黄绿素重现荧光,据此建立了测定莫西沙星的新方法.将该方法用于片剂和尿液中莫西沙星的测定,结果满意.实验表明,该方法简单、快速、灵敏、准确.

  1. 左氧氟沙星与莫西沙星治疗耐药性肺结核的疗效和安全性比较%Comparison of Efficacy and Safety of Levofloxacin and Moxifloxacin in the Treatment of Patients with Drug-resistant Tuberculosis

    Institute of Scientific and Technical Information of China (English)

    朱刚

    2016-01-01

    OBJECTIVE:To compare the efficacy and safety of levofloxacin and moxifloxacin in the treatment of patients with drug-resistant tuberculosis. METHODS:148 patients with drug-resistant tuberculosis were divided into observation group(74 cases) and control group(74 cases). All patients were treated with 300 mg Aminosalicylic acid isoniazid tablet+250 mg Ethambutol hydro-chloride tablet+200 mg Pyrazinamide tablet+200 mg Protionamide tablet,3 times a day,treatment for 3 months;based on it,obser-vation group was orally given 0.4 g Moxifloxacin hydrochloride tablet,once a day;control group was orally given 0.5 g Levofloxa-cin hydrochloride tablet,once a day. They were treated for 12 months. The total efficacy,sputum negative convevsion rate after 1, 4,8 and 12 months,X-ray cavities lesion improvement and incidence of ADR in 2 groups were observed. RESULTS:The total effi-cacy,sputum negative conversion rate after 1,4,8 and 12 months,and cavity closure and reduced proportion in observation group were significantly higher than control group,the empty change and increase proportion,incidence of ADR were significantly lower control group,the differences were statistically significant (P<0.05). CONCLUSIONS:Based on conventional treatment, moxifloxacin shows better efficacy and safety than levofloxacin in the treatment of patients with drug-resistant tuberculosis.%目的:比较左氧氟沙星与莫西沙星治疗耐药性肺结核的疗效和安全性。方法:148例耐药性肺结核患者随机分为观察组(74例)和对照组(74例)。两组患者均给予氨基水杨酸异烟肼片300 mg+盐酸乙胺丁醇片250 mg+吡嗪酰胺片200 mg+丙硫异烟胺片200 mg,均为每日3次,连用3个月。在此基础上,观察组患者口服盐酸莫西沙星片0.4 g,每日1次;对照组患者口服盐酸左氧氟沙星片0.5 g,每日1次。两组均治疗12个月。比较两组患者的临床疗效,治疗1、4、8、12个月后痰菌转阴率,X线空洞病

  2. A novel oral preparation of human growth hormone (hGH) is absorbed and increases serum IGF-I levels after 7 days administration to GH-deficient adults

    DEFF Research Database (Denmark)

    Laursen, Torben; Mindeholm, Linda; Haemmerle, Sibylle;

    2007-01-01

    Growth hormone deficient (GHD) patients are currently effectively treated with daily subcutaneous (sc) injections of hGH in the evening, but alternative routes would be attractive. An oral formulationulation of hGH, using an amino-caprilic acid derivative (5-CNAC, Emisphere's eligen® technology) as...... carrier has recently been developed. The aim of this study was to determine if this oral formulation of hGH could be absorbed and be bioactive. Eight GHD men (mean age 50 years) receiving sc hGH therapy were withdrawn from therapy for 7 days and then treated for 7 days orally with tablets of HGH191...... (Novartis). Each tablet contained 100 mg of hGH and 200 mg 5-CNAC. One tablet was given at 08 h and 17 h, and two tablets at 22 h to match nocturnal GH secretion. The patients were studied thrice for 24 hours: at day 7 of washout, at days 1 and 7 of treatment. GH peaks were recorded in all patients...

  3. 耐多药肺结核病治疗方案中使用莫西沙星的临床效果%Clinical effect of applying moxifloxacin in treatment scheme for multi-drug resistant tuberculosis

    Institute of Scientific and Technical Information of China (English)

    吴颖

    2015-01-01

    Objective To compare and analyze clinical effects of applying moxifloxacin and levofloxacin in the treatment scheme for multi-drug resistant tuberculosis. Methods A total of 48 patients with multi-drug resistant tuberculosis were divided into two groups, as moxifloxacin group with 24 cases and levofloxacin group with 24 cases. Both groups received basic antituberculous scheme by isoniazid aminosalicylate tablets, ethambutol, pyrazinamide, and protionamide. The levofloxacin group was treated by additional levofloxacin tablets, and the moxifloxacin group received additional moxifloxacintablets. Curative effects and adverse reactions were observed after 12 months of treatment. Results The total effective rate after treatment of the moxifloxacin group was 91.7%, which was higher than 75.0%of the levofloxacin group, and the difference had statistical significance (P0.05). Conclusion Moxifloxacin has remarkably better effect than levofloxacin in the treatment scheme for multi-drug resistant tuberculosis.%目的:对比分析耐多药肺结核病治疗方案中使用莫西沙星和左氧氟沙星的临床效果。方法48例耐多药肺结核病患者分为两组,即莫西沙星组24例和左氧氟沙星组24例,两组患者均以对氨基水杨酸异烟肼片、乙胺丁醇、吡嗪酰胺和丙硫异烟胺为基础抗结核方案,左氧氟沙星组联合应用左氧氟沙星片,莫西沙星组联合应用莫西沙星片,治疗12个月观察疗效和不良反应。结果治疗后莫西沙星组总有效率为91.7%,高于左氧氟沙星组的75.0%,差异有统计学意义(P0.05)。结论在耐多药肺结核病治疗方案中莫西沙星的疗效明显优于左氧氟沙星。

  4. Effect of moxifloxacin on treatment of pulmonary infections after esophageal cancer resection%莫西沙星对食管癌术后肺部感染的疗效分析

    Institute of Scientific and Technical Information of China (English)

    陈勇杰; 俞达辉; 褚文炎; 吴道立

    2013-01-01

    目的 分析莫西沙星治疗食管癌术后肺部感染的疗效,评价其在临床应用的价值.方法 选取2007年6月-2012年6月收治的食管癌术后发生肺部感染208例患者,将其随机分为3组即莫西沙星组76例、舒巴哌酮组70例、左氧氟沙星组62例,分别使用莫西沙星、舒巴哌酮、左氧氟沙星治疗食管癌术后的肺部感染,比较3组应用后的临床疗效、并计算细菌学清除率和安全性等.结果 莫西沙星组、舒巴哌酮组、左氧氟沙星组的有效率分别为93.42%、88.57%、74.19%,3组细菌清除率分别为92.75%、92.06%、70.18%,莫西沙星组与舒巴哌酮组疗效相当,差异无统计学意义;莫西沙星组与左氧氟沙星组相比,莫西沙星组疗效较好,差异有统计学意义(P<0.05).结论 莫西沙星对于食管癌术后的肺部感染的疗效与舒巴哌酮相当,但要好于三代头孢菌素,莫西沙星的不良反应较少、安全性高.%OBJECTIVE To explore the efficacy of moxifloxacin in the treatment of pulmonary infections after esophageal cancer resection so as to evaluate the value in clinical application.METHODS A total of 208 cases of patients with pulmonary infections,who underwent esophageal cancer resection from Jun 2007 to Jun 2012,were enrolled in the study and were randomly divided into the the moxifloxacin group(n =76),cefoperazune-sulbactam sodium group(n =70),and the levofloxacin group(n =62),which were respectively treated with moxifloxacin,cefoperazune-sulbactam,or levofloxacin for the pulmonary infections.The clinical efficacy after the treatment was compared between the three groups,the bacterial clearance rate was calculated,and the safety was evaluated.RESULTS The effective rates of the moxifloxacin group,cefoperazune-sulbactam group,and the levofloxacin group were 93.42%,88.57%,and 74.19%,respectively,the bacterial clearance rates of the three groups were 92.75%,92.06%,and 70.18

  5. Simple and sensitive stability-indicating ion chromatography method for the determination of cyclopropylamine in nevirapine and moxifloxacin hydrochloride drug substances.

    Science.gov (United States)

    Kothapalli, Pavan Kumar S R; Khagga, Mukkanti; Mekala, Nageswara Rao; Sigamani, John Prasanna; Vundavilli, Jagadeesh Kumar; Masani, Narendra Kumar; Sharma, Hemant Kumar

    2012-01-01

    A simple and sensitive ion chromatography method has been developed for the determination of cyclopropylamine (CPA) in nevirapine (NEV) and moxifloxacin HCl (MOX) pharmaceutical drug substances. Efficient chromatographic separation was achieved on a Metrosep C4, 5 μm (250 mm × 4.0 mm) column. The mobile phase consists of 5 mM hydrochloric acid containing 10% (v/v) acetonitrile and was delivered in an isocratic mode at a flow rate of 0.9 mL min(-1) at 27°C. A conductometric detector was used for the detection of the analyte. The drug substances were subjected to stress conditions including oxidation, thermal, photolytic and humidity for the evaluation of the stability-indicating nature of the method. The method was validated for specificity, precision, linearity, accuracy and solution stability. The limit of detection (LOD) and limit of quantification (LOQ) values are 0.10 μg mL(-1) and 0.37 μg mL(-1) respectively. The linearity range of the method is between 0.37 μg mL(-1) and 1.5 μg mL(-1) and the correlation coefficient is found to be 0.9971. The average recoveries of CPA in NEV and MOX are 97.0% and 98.0%, respectively.

  6. Design, characterization, and aerosolization of organic solution advanced spray-dried moxifloxacin and ofloxacin dipalmitoylphosphatidylcholine (DPPC) microparticulate/nanoparticulate powders for pulmonary inhalation aerosol delivery

    Science.gov (United States)

    Duan, Jinghua; Vogt, Frederick G; Li, Xiaojian; Hayes, Don; Mansour, Heidi M

    2013-01-01

    The aim of this study was to design and develop respirable antibiotics moxifloxacin (MOXI) hydrochloride and ofloxacin (OFLX) microparticles and nanoparticles, and multifunctional antibiotics particles with or without lung surfactant 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) for targeted dry powder inhalation delivery as a pulmonary nanomedicine. Particles were rationally designed and produced by advanced spray-drying particle engineering from an organic solution in closed mode (no water) from dilute solution. Scanning electron microscopy indicated that these particles had both optimal particle morphology and surface morphology, and the particle size distributions were suitable for pulmonary delivery. Comprehensive and systematic physicochemical characterization and in vitro aerosol dispersion performance revealed significant differences between these two fluoroquinolone antibiotics following spray drying as drug aerosols and as cospray-dried antibiotic drug: DPPC aerosols. Fourier transform infrared spectroscopy and confocal Raman microspectroscopy were employed to probe composition and interactions in the solid state. Spray-dried MOXI was rendered noncrystalline (amorphous) following organic solution advanced spray drying. This was in contrast to spray-dried OFLX, which retained partial crystallinity, as did OFLX:DPPC powders at certain compositions. Aerosol dispersion performance was conducted using inertial impaction with a dry powder inhaler device approved for human use. The present study demonstrates that the use of DPPC offers improved aerosol delivery of MOXI as cospray-dried microparticulate/nanoparticulate powders, whereas residual partial crystallinity influenced aerosol dispersion of OFLX and most of the compositions of OFLX:DPPC inhalation powders. PMID:24092972

  7. Comparative Activity of Ciprofloxacin, Levofloxacin and Moxifloxacin against Klebsiella pneumoniae, Pseudomonas aeruginosa and Stenotrophomonas maltophilia Assessed by Minimum Inhibitory Concentrations and Time-Kill Studies

    Science.gov (United States)

    Grillon, Antoine; Schramm, Frédéric; Kleinberg, Magali; Jehl, François

    2016-01-01

    The aim of this study was to compare the in vitro susceptibility of Klebsiella pneumoniae, Pseudomonas aeruginosa and Stenotrophomonas maltophilia to three fluoroquinolones. The minimum inhibitory concentrations (MICs) to ciprofloxacin, levofloxacin and moxifloxacin were examined by E-test® for a total of 40 K. pneumoniae strains, 40 S. maltophilia strains and 40 P. aeruginosa strains. Then, the bactericidal activity of these fluoroquinolones was investigated on five strains of each bacterial species by means of time-kill curves. For K. pneumoniae and P. aeruginosa, the distance of the measured MIC from the clinical break-point is a good indicator of the bactericidal activity for ciprofloxacin and levofloxacin as obtained in our experiments. The lower the MIC, the better the bactericidal activity in term of CFU Log decreases. If MIC of ciprofloxacin and levofloxacin against the considered bacteria are far from clinical breakpoint, these two antibiotics are equivalent. According to our MIC50 and modal MIC, the breakpoints of both ciprofloxacin and levofloxacin seem to be somewhat high and data suggest reducing them. On S. maltophilia, none of the tested antibiotics showed a satisfactory activity. PMID:27257956

  8. Simultaneous determination of moxifloxacin and H2 receptor antagonist in pharmaceutical dosage formulations by RP-HPLC: application to in vitro drug interactions

    Directory of Open Access Journals (Sweden)

    Najma Sultana

    2011-01-01

    Full Text Available Simultaneous determination of moxifloxacin (MOX and H2-antagonists was first time developed in bulk and formulations. Purospher STAR C18 (250 x 4.6 mm, 5 μm column was used. The mobile phase (methanol: water: ACN, 60:45:5 v/v/v, pH 2.7 was delivered at a flow rate of 1.0 mL min-1, eluent was monitored at 236, 270 and 310 nm for cimetidine, famotidine and ranitidine, respectively. The proposed method is specific, accurate (98-103%, precise (intra-day and inter-day variation 0.098-1.970% and linear (r>0.998. The LOD and LOQ were 0.006-0.018 and 0.019-0.005 μg mL-1, respectively. The statistical parameters were applied to verify the results. The method is applicable to routine analysis of formulations and interaction of MOX with H2-antagonist.

  9. 莫西沙星序贯治疗老年社区获得性肺炎的药物经济学评价%Pharmacoeconomic efficacy of moxifloxacin sequential therapy for community-acquired pneumonia in elderly patients

    Institute of Scientific and Technical Information of China (English)

    王媛媛; 刘丽萍; 操艺; 洪钰; 李丽莉

    2012-01-01

    目的 对莫西沙星两种不同给药方案治疗老年社区获得性肺炎进行药物经济学评价.方法 将80例患者随机分为莫西沙星序贯治疗组(序贯组)和莫西沙星静脉滴注组(对照组),每组各40例.序贯治疗组给予莫西沙星注射液0.4 g,静脉滴注,1次·d-1,5 d后,病情好转,改为莫西沙星片0.4 g,口服,1次·d-1,总疗程11 d;对照组给予莫西沙星注射液0.4 g,静脉滴注,1次·d-1,总疗程11 d.评价临床疗效及药物经济学.结果 序贯组和对照组痊愈率分别为57.5%、62.5%(P>0.05),有效率分别为90.0%、92.5%(P>0.05),不良反应发生率分别为7.5%、10%(P>0.05).序贯治疗组的成本-效果比为2 000.0,低于对照组(3 864.9).结论 莫西沙星序贯治疗老年社区获得性肺炎疗效高、安全、经济.%Objective To evaluate pharmacoeconomic efficacy of two different dosage regimens of moxifloxacin in the treatment of community acquired pneumonia in elderly patients. Method A total of 80 patients were randomly enrolled into 2 groups: moxifloxacin injection followed by tablet ( sequential group ) and moxifloxacin injection group ( control group ). Moxifloxacin was administered by intravenous infusion,400 mg/250 ml once daily for 11 days in control group( n =40 ). In sequential group( n =40 ),moxifloxacin was given by intravenous infusion 400 mg/250 ml, once daily for 5 days, followed by oral administration at the same dose. The total treatment duration was also 11 days. After the end of therapy,to the clinical efficacy and pharmacoeconomic efficacy were evaluation. Results For sequential group vs. Control group,the cute rate was 57. 5% vs 62. 5%( P>0. 05 ),the effective rate was 90. 0% vs 92. 5%( P >0. 05 ),and the incidence of ADRs was 7. 5% vs 10% ( P > 0. 05 ). The cost-effectiveness of sequential group( 2 000. 0 ) was lower than control group ( 3864. 9 )o Conclusion Moxifloxacin sequential therapy is efficacious, safety and cost-effective for community

  10. 莫西沙星在中国健康受试者体内的药代动力学研究%Pharmcokinetics of moxifloxacin in Chinese healthy volunteers

    Institute of Scientific and Technical Information of China (English)

    刘银萍; 秦玉花; 白楠; 唐铭婧; 王睿; 赵红伟

    2015-01-01

    Objective To study the pharmacokinetics of oral administra-tion of moxifloxacin in Chinese healthy volunteers .Methods The study was designed as an open , randomized , parallel and single -dose cycle study.The volunteers were administered with a single dose of moxifloxa-cin 400 mg for consistent four days , respectively.The plasma concentra-tions of moxifloxacin were determined by LC -MS/MS method. The pharmacokinetic parameters of moxifloxacin were calculated by WinNon-lin 5.2.1 software.Results Six subjects quit from the study , and the main pharmacokinetic parameters of the remaining 42 subjects were as follows:t1/2 was (11.71 ±3.53 ) h, Cmax was (0.51 ±0.19 ) ×10 -4 ng・ mL-1, tmax was(2.94 ±1.41) h, MRT0-24 hwas(18.02 ±4.45)h, CLz/F was ( 0.84 ± 0.75 ) × 10 -4 mL ・ h-1 , AUC0-24 h was (5.82 ±1.82 ) ×10 -4 ng・ mL-1・ h.Conclusion The characteristics of oral moxifloxacin 400 mg has shown a high blood concentration , long half-life time and it can be well tolerated.The doses adopted in foreign study is recommended .There was a gender difference in volunteers when with same dose of moxifloxacin .%目的:评价中国健康受试者连续4 d单剂量口服盐酸莫西沙星片400 mg的药代动力学。方法开放、随机、平行、单次、单周期试验设计。48名健康受试者,男女各半,单剂量口服盐酸莫西沙星片400 mg,连续4 d,用高效液相色谱串联质谱法测定血药浓度,用 WinNonlin 5.2.1程序计算药代动力学参数。结果有6例受试者中途退出,其余42名健康受试者口服莫西沙星后的主要药代动力学参数如下, t1/2为(11.71±3.53) h, Cmax为(0.51±0.19)×10-4 ng・ mL-1, tmax为(2.94±1.41) h, MRT 0-24 h为(18.02±4.45) h, CLz/F 为(0.84±0.75)×10-4 mL・ h-1,AUC0-24 h为(5.82±1.82)×10-4 ng・ mL-1・ h。结论我国健康受试者口服莫西沙星血药浓度高、半衰期长、耐受性好,可参照国外剂量,并且

  11. The efficacy of moxifloxacin in reducing conjunctival bacterial flora in vivo%莫西沙星对减少结膜囊细菌效果的临床实验研究

    Institute of Scientific and Technical Information of China (English)

    胡楠; Herminia Mino de Kaspar; Christopher N.Ta

    2008-01-01

    目的 探讨术前应用0.5%莫西沙星眼药水对减少结膜囊细菌的效果.方法 对96例(98只眼)行眼前节手术的患者,随机分为A、B两组,分别于术前3 d和1 d用0.5%莫西沙星眼药水点眼,用药及手术前后取结膜囊标本做肉汤培养基培养、血平板培养及厌氧菌培养,对阳性结果行药物敏感性试验.结果 肉汤培养结果示:用药3 d或1 d后(T1)A、B两组的结膜囊细菌阳性率均低于用药前(P=0.000),术前1 h用药及聚维酮碘冲洗结膜囊后(T2),A、B两组的阳性率均低于T1(P=0.005;P=0.016),手术完成时(T3)A、B两组的阳性率与T2无差异(P=0.399;P=0.140),在各个时间点,A、B两组之间结膜囊细菌培养阳性率均差异无统计学意义(P>0.05).血平板培养结果示:用药后(T1)两组结膜囊细菌阳性率均明显下降(P<0.001),CFUs>10的菌株消失.厌氧菌培养结果示:用药后(T1)两组结膜囊细菌阳性率无明显下降(P>0.05).结论 术前1 d局部应用0.5%莫西沙星眼药水可以明显减少结膜囊需氧菌.%Objective To investigate the efficacy of 0.5% moxifloxacin in reducing the rates of positive conjunctival bacteria cultures.Methods 96 patients (98 eyes) were randomized to 2 groups and the patients received topical 0.5% moxifloxacin 3d (group A) or 1d (group B) before surgery.Conjunctival cultures were obtained at 4 separate time points and were inoculated in solid and liquid culture media for both aerobic and anaerobic bacteria.The presence of bacteria was determined, quantified,and antibiotic susceptibility testing was performed.Results After application of topical 0.5% moxifloxacin (T1),the rates of positive conjunctival cultures were statistically lower in both groups (P=0.000).After 1h preoperative antibiotic application and povidone iodine irrigation,the rates of positive conjunctival cultures were statistically lower than T1 in both groups (P=0.005;P=0.016).At the conclusion of surgery(T3),there was no

  12. 莫西沙星有效治疗老年人社区获得性肺炎%Moxifloxacin in the Treatment of Elderly Patients with Community Acquired Pneumonia

    Institute of Scientific and Technical Information of China (English)

    伏杭江; 游云鹏

    2011-01-01

    Community acquired pneumonia is a common infectious disease in the elderly patients.The new generation fluoroquinolone moxifloxacin has a definite clinical effect and high safety, can be widely used in the treatment of elderly patients with community acquired pneumonia.%社区获得性肺炎是老年人常见的感染性疾病.新一代的氟喹诺酮类药物莫西沙星临床疗效确切、安全性高,可作为老年人社区获得性肺炎经验治疗用药.

  13. Wild-Type and Non-Wild-Type Mycobacterium tuberculosis MIC Distributions for the Novel Fluoroquinolone Antofloxacin Compared with Those for Ofloxacin, Levofloxacin, and Moxifloxacin.

    Science.gov (United States)

    Yu, Xia; Wang, Guirong; Chen, Suting; Wei, Guomei; Shang, Yuanyuan; Dong, Lingling; Schön, Thomas; Moradigaravand, Danesh; Parkhill, Julian; Peacock, Sharon J; Köser, Claudio U; Huang, Hairong

    2016-09-01

    Antofloxacin (AFX) is a novel fluoroquinolone that has been approved in China for the treatment of infections caused by a variety of bacterial species. We investigated whether it could be repurposed for the treatment of tuberculosis by studying its in vitro activity. We determined the wild-type and non-wild-type MIC ranges for AFX as well as ofloxacin (OFX), levofloxacin (LFX), and moxifloxacin (MFX), using the microplate alamarBlue assay, of 126 clinical Mycobacterium tuberculosis strains from Beijing, China, of which 48 were OFX resistant on the basis of drug susceptibility testing on Löwenstein-Jensen medium. The MIC distributions were correlated with mutations in the quinolone resistance-determining regions of gyrA (Rv0006) and gyrB (Rv0005). Pharmacokinetic/pharmacodynamic (PK/PD) data for AFX were retrieved from the literature. AFX showed lower MIC levels than OFX but higher MIC levels than LFX and MFX on the basis of the tentative epidemiological cutoff values (ECOFFs) determined in this study. All strains with non-wild-type MICs for AFX harbored known resistance mutations that also resulted in non-wild-type MICs for LFX and MFX. Moreover, our data suggested that the current critical concentration of OFX for Löwenstein-Jensen medium that was recently revised by the World Health Organization might be too high, resulting in the misclassification of phenotypically non-wild-type strains with known resistance mutations as wild type. On the basis of our exploratory PK/PD calculations, the current dose of AFX is unlikely to be optimal for the treatment of tuberculosis, but higher doses could be effective. PMID:27324769

  14. One Case of Transient Epileptic Seizures Caused by Intravenous Drip of Moxifloxacin%静脉滴注莫西沙星致短暂癫痫样发作1例

    Institute of Scientific and Technical Information of China (English)

    蔡然; 张杰根

    2013-01-01

    A 47-year-old male patient with pneumonia was given moxifloxacin hydrochloride and sodium chloride injection ( 400 mg ) via intravenous drip . On the next day , convulsions and loss of consciousness occurred during the process of receiving the intravenous drip of moxifloxacin hydrochloride and sodium chloride injection , which lasted for about 20 seconds and then relieved . The above mentioned symptoms didn ’ t happen when the dripping speed was slowed down but without withdrawl of the injection .%1例47岁男性患者,因肺炎给予盐酸莫西沙星氯化钠注射液(拜复乐)400 mg ,1次/d静脉滴注。用药次日,患者再次输注拜复乐过程中发生肢体抽搐,意识丧失,持续约20秒钟缓解,后调慢滴速,未予停药,上述症状未再发生。

  15. 莫西沙星序贯治疗老年社区获得性肺炎的疗效研究%Sequential moxifloxacin therapy in treatment of elderly patients with community-acquired pneumonia

    Institute of Scientific and Technical Information of China (English)

    王玉珠

    2011-01-01

    Objective To evaluate the efficacy, safety and cost of sequential moxifloxacin therapy in elder patients with community acquired pneumonia compared with moxifloxacin injection. Methods 80 elder patients with community acquired pneumonia were randomly divided into two groups, which were treated with moxifioxacin injection followed by tablet (sequential group) or moxifloxacin injection group (control group). Results The effective rate for the sequential group was 90.00%. While for the control group was 92.50%. There was no statistically significant difference between the two groups (P>0.05 ). The bacterial eradication rate in the two groups was 88. 23% and 88.89%, respectively.There was also no significant difference between the two group ( P>0.05 ). The average cost and the incidence of adverse drug reaction of the sequential group was lower than that of the control group. Conclusion The sequential moxifloxacin therapy for CAP is safe, effective, and cost-saved compared with full-course moxifloxacin infusion.%目的 研究莫西沙星序贯治疗老年社区获得性肺炎的疗效.安全性和治疗费用.方法 80例老年社区获得性肺炎患者先予以莫西沙星注射液400 mg/250 ml静脉滴注.每天1次,治疗5~7 d.临床症状改善后,随机分成序贯组和对照组.序贯组40例改口服莫西沙星片剂400 mg,每天1次.对照组40例继续静脉滴注莫西沙星注射液400mg/250 ml,每天1次.两组总疗程10 d.观察疗效、药物的不良反应和治疗费用.结果 两组的有效率无明显差异(P>0.05),分别为90.00%和92.50%.两组细菌清除率分别为88.23%和88.89%,无明显差异(P>0.05).序贯组的治疗费用、不良反应均明显低于对照组(P<0.05).结论 莫西沙星序贯治疗老年社区获得性肺炎疗效确切、安全,且节约费用.

  16. Design, characterization, and aerosolization of organic solution advanced spray-dried moxifloxacin and ofloxacin dipalmitoylphosphatidylcholine (DPPC microparticulate/nanoparticulate powders for pulmonary inhalation aerosol delivery

    Directory of Open Access Journals (Sweden)

    Duan J

    2013-09-01

    Full Text Available Jinghua Duan,1,2 Frederick G Vogt,3 Xiaojian Li,1 Don Hayes Jr,4,5 Heidi M Mansour6 1University of Kentucky College of Pharmacy, Department of Pharmaceutical Sciences – Drug Development Division, Lexington, KY, USA; 2University of Washington-Seattle, College of Pharmacy, Department of Pharmaceutics, Seattle, WA, USA; 3GlaxoSmithKline, Analytical Sciences, Product Development, King of Prussia, PA, USA; 4The Ohio State University College of Medicine, Departments of Pediatrics and Internal Medicine, Nationwide Children's Hospital Lung and Heart-Lung Transplant Programs, Columbus, OH, USA; 5The Ohio State University, Davis Heart and Lung Research Institute, Columbus, OH, USA; 6The University of Arizona–Tucson, College of Pharmacy, Skaggs Center of Pharmaceutical Sciences, Tucson, AZ, USA Abstract: The aim of this study was to design and develop respirable antibiotics moxifloxacin (MOXI hydrochloride and ofloxacin (OFLX microparticles and nanoparticles, and multifunctional antibiotics particles with or without lung surfactant 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC for targeted dry powder inhalation delivery as a pulmonary nanomedicine. Particles were rationally designed and produced by advanced spray-drying particle engineering from an organic solution in closed mode (no water from dilute solution. Scanning electron microscopy indicated that these particles had both optimal particle morphology and surface morphology, and the particle size distributions were suitable for pulmonary delivery. Comprehensive and systematic physicochemical characterization and in vitro aerosol dispersion performance revealed significant differences between these two fluoroquinolone antibiotics following spray drying as drug aerosols and as cospray-dried antibiotic drug: DPPC aerosols. Fourier transform infrared spectroscopy and confocal Raman microspectroscopy were employed to probe composition and interactions in the solid state. Spray-dried MOXI was

  17. 前房内给予莫西沙星预防眼内炎在白内障手术中的应用%Application of intracameral moxifloxacin to prevent endophthalmitis in cataract surgery

    Institute of Scientific and Technical Information of China (English)

    Servet Cetinkaya; Yasemin F Cetinkaya; Nursen O Acir; Zeynep Dadaci

    2015-01-01

    目的:评价在白内障手术中采用前房内应用莫西沙星预防眼内炎的安全性和效果。  方法:选取2012年1-6 mo在我院行白内障手术患者65例65眼。部分患者手术结束时前房给予莫西沙星,另外一部分作为对照组未使用莫西沙星。对患者术前和术后logMAR最佳矫正视力、眼内压、角膜水肿、前房形态进行检查。  结果:共33例患者(男19,女14)使用了莫西沙星,平均年龄64.81±11.61(41~82)岁;32例患者(男15,女17)没有使用,平均年龄65.43±11.10(42-81)岁。患者的年龄(P=0.827)和性别(P=0.396)无统计学差异。术前两组的最佳矫正视力均接近20/130。术后,莫西沙星组和对照组的最佳矫正视力分别为20/25和20/23( P=0.160)。术前莫西沙星组眼内压为14.93±2.77(11-21) mmHg,对照组为15.06±2.42(12-21)mmHg(P=0.850)。术后,两组眼内压均无统计学差异[莫西沙星组:14.06±2.31(10-19) mmHg;对照组:14.03±2.36(10-19) mmHg ( P=0.726)]。两组角膜水肿(P=0.623)与前房细胞发生率(P=0.726)均无统计学差异。  结论:白内障手术后给予莫西沙星预防眼内炎是安全而有效的。%AIM:To evaluate the safety and efficacy of intracameral moxifloxacin in preventing endophthalmitis after cataract surgery. METHODS:Sixty-five eyes of 65 patients underwent cataract surgery between January and June 2012. Some patients received intracameral moxifloxacin at the end of surgery, while others did not ( controls ) . Pre - and postperative logarithm of the minimum angle of resolution ( logMAR ) best corrected visual acuity ( BCVA) , intraocular pressure ( IOP) , corneal edema, and anterior chamber ( AC) status were examined. RESULTS: Thirty - three patients ( 19 males, 14 females); average age, 64. 81±11. 61y ( range: 41-82y) received moxifloxacin and 32 patients ( 15 males, 17 females); average age, 65. 43±11. 10y ( range: 42-81y) did not. The differences in patient age (P=0. 827

  18. Clinical analysis on transcutaneous bilirubin value of normal neonates at 7 days after birth%正常新生儿生后7天经皮胆红素值的临床分析

    Institute of Scientific and Technical Information of China (English)

    王金秀; 王瑛; 江华; 张琳; 刘滢; 丁雪晶

    2011-01-01

    Objective: To provide the transcutaneous bilirubin (TcB) value of normal neonates at 7 days after birth in Changzhou region, obtain the average peak value of TcB, provide a basis for clinical diagnosis, draft an hour - specific TcB percentile nomogram suitable for the neonates in the region. Methods: 7 482 TcB values were obtained from 679 healthy full term neonates and near full term neonates by JH20 - 1B percutaneous jaundice apparatus from January to March in 2010, the detection time was from 12 hours after birth until 152 hours after birth. Every TcB percentile for each designated time was calculated and used for drafting an hour - specific nomogram. Results: TcB values varied at different time points among the normal neonates at 12 ~ 152 hours after birth, peaked at 116 hours after birth, the proportion of neonatal hyperbilirubinemia (TcB>12.9 mg/dL) was 8.4% (57/679) , no bilirubin encephalopathy occurred during follow - up after leaving the hospital TcB nomogram reflected the natural history of TcB among the normal neonates at 7 days after birth. The average peak value of bilirubin was (10.0 ±2. 2) mg/dL, the P40, P75 and P95 of bilirubin were 8. 3 mg/dL, 10. 8mg/dL and 12.6 mg/dL, respectively. Among the perinatal factors, TcB level was significantly associated with gestational age, premature rupture of membrane and cephalhematoma (P < 0. 05) . Conclusion: The appearance time and peak time of neonatal jaundice in Changzhou region are similar to the published reports at home and abroad; the diagnostic criterion of neonatal hyperbilirubinemia in China should be higher than current criterion, thus, the interventional standard of hyperbilirubinemia should be improved correspondingly.%目的:提供常州地区正常新生儿生后7天经皮胆红素(TcB)值,得出胆红素的平均峰值,为临床诊断奠定基础并制定一份适合本地区新生儿小时TcB百分位列线图.方法:2010年1~3月679例健康足月和近足月新生儿采用JH20 - 1B

  19. 含莫西沙星强化期抗结核方案用于初治肺结核的效果评价%Therapeutic effect of the regimen containing Moxifloxacin in intensive phase for pulmonary tuberculosis patients

    Institute of Scientific and Technical Information of China (English)

    贾维

    2011-01-01

    Objective To explore the effect and safety of the regimen containing Moxifloxacin in intensive phase for pulmonary tuberculosis patients. Methods 88 cases with initial tuberculosis(each group of 44 cases) were studied in comparison in the same period. The treatment group contained isoniazid, rifampicin and moxifloxacin of antituberculosis regime and the control group contained isoniazid,rifampicin and pyrazineamide. Results After 2 months intensive phase anti-tubercular treatment ,the sputum negative conversion rates was 95.0% and control's was 94.4%(P > 0.05); the absorb rate of lesions: the treatment group 77.3 % and the control group 79.5%; the liver damage rate: the treatment group and control group was 68.2% ,34.1% respectively(P < 0.05). Conclusion Anti-tuberculosis intensive phase regimes containing moxifloxacin for tuberculosis treatment was safe and effective in initial treatment of pulmonary tuberculose.%目的 探讨强化期含莫西沙星用于初治肺结核的有效性和安全性.方法 对88例初治肺结核进行了同期对照配对研究(每组44例).其中治疗组为含莫西沙星(异烟肼、利福平、莫西沙星)的抗结核方案,对照组为异烟肼、利福平、吡嗪酰胺的治疗方案.结果 抗结核治疗2个月,治疗组与对照组痰菌阴转率(95.0%与94.4%)、病灶吸收好转率(77.3%与79.5%)差异均无统计学意义(均P>0.05));肝损率治疗组34.1%,对照组68.2%,差异有统计学意义(P<0.05).结论 含莫西沙星的强化期联合抗结核方案用于初治肺结核的治疗安全有效.

  20. 莫西沙星在支原体肺炎治疗中的运用研究%The Applied Research on Moxifloxacin Medicine Treatment of Mycoplasma Pneumonia

    Institute of Scientific and Technical Information of China (English)

    王兵

    2015-01-01

    目的:研究莫西沙星在支原体肺炎治疗中的运用效果。方法搜集2013年6月~2014年7月我院接收的支原体肺炎84例患者,按照就诊日期单双号分为对照组、研究组。给予对照组42例阿奇霉素,给予研究组42例莫西沙星。观察对照组与研究组的治疗效果,并对比。结果与对照组相比,研究组治疗有效率较高,症状改善较迅速,不良反应较少,有明显差异,有统计学意义(P<0.05)。结论莫西沙星治疗支原体肺炎疗效确切,且可靠、安全。%Objective The applied effect of moxifloxacin medicine treatment in mycoplasma pneumonia is to be analyzed. Methods Choose 84 patients with mycoplasma pneumonia who are treated in hospital from june 2013 to july 2014 and separate them into study group and control group according to patients’ treatment date number (odd and even numbers), patients in control group are given azithromycin medicine treatment and patients in study group are given moxifloxacin medicine treatment, and then observe and compare the treatment effects of the two groups. Results Compared to control group, the efficiency of study group is much higher, and patients’ symptoms in study group are improved quickly with less side effects. such a differential has statistic value (P<0.05). Conclusion Moxifloxacin medicine treatment is quite effective to cure mycoplasma pneumonia, it is reliable and safe, which is worthy of being promoted and applied.

  1. 莫西沙星治疗艾滋病合并下呼吸道感染的临床观察%Clinical Observation of Moxifloxacin in the Treatment of Acquired Immunodeficiency Syndrome Complicated with Lower Respiratory Tract Infection

    Institute of Scientific and Technical Information of China (English)

    李洪智

    2011-01-01

    目的探讨莫西沙星治疗敏感菌引起的艾滋病合并下呼吸道感染的疗效.方法将184例艾滋病合并下呼吸道感染患者随机分成两组,治疗组(n=94)予莫西沙星静脉滴注,对照组(n=90)予盐酸左氧氟沙星静脉滴注,疗程均为7 ~12 d.结果两组治疗效果比较差异有统计学意义(P<0.05).细菌清除率:治疗组92.3%,对照组88.5%,两组比较差异无统计学意义(P>0.05);不良反应发生率:治疗组4.3%,对照组5.6%,两组比较差异无统计学意义(P>0.05).结论莫西沙星治疗艾滋病合并下呼吸道感染疗效确切,安全性好.%Objective To evaluate the efficacy and safety of moxifloxacin therapy in the treatment of patients with acquired immunodeficiency syndrome( AIDS )complicated with lower respiratory tract infection.Methods 184 patients with AIDS complicated with lower respiratory tract Infection were randomly divided into two groups. In treatment group( n = 94 ),moxifloxacin was administered by intravenous infusion. In control group( n = 90 ), levofloxacin was administered by intravenous infusion. The total treatment duration was 7 ~ 12 days. Results There was statistically significant difference( P < 0.05 )in treatment effectiveness. The bacterial clearance rates were 92.3% in treatment group,88.5% in control group. There was no significant difference( P > 0.05 ). The incidence of adverse drug reactions of two groups were 4.3% in treatment group and 5.6% in control group,respectively. There was no significant difference( P > 0.05 ). Conclusion moxifloxacin intravenous infusion in the treatment of AIDS complicated with lower respiratory tract infection was more effective and safe.

  2. 中药联合莫西沙星治疗老年性肺炎临床疗效分析%Analysis of Clinical Efficacy of Traditional Chinese Medicine Combined with Moxifloxacin in the Treatment of Senile Pneumonia

    Institute of Scientific and Technical Information of China (English)

    徐汉明

    2013-01-01

      目的:探讨中药联合莫西沙星治疗老年性肺炎的临床疗效。方法:老年性肺炎56例平均分为观察组(采用联合用药治疗)与对照组(单纯采用莫西沙星治疗),2周疗程后对比两组的临床疗效。结果:观察组的痊愈20例,显效5例,好转3例,总有效率为89.28%;对照组痊愈12例,显效10例,好转2例,无效4例,总有效率为78.57%;观察组临床疗效明显高于对照组(P<0.05)。结论:肺炎老年患者临床联合应用中药及莫西沙星可显著提高临床疗效,值得推广应用。%Objective:To investigate the clinical efficacy of traditional Chinese medicine combined with moxifloxacin in the treat-ment of senile pneumonia. Methods:56 cases of senile pneumonia were divided into observation group (used drug combination treat-ment) and control group (simple used moxifloxacin treatment), the clinical effects were compared between the two groups after 2 weeks. Results:20 cases in observation group were cured, 5 were effective, 3 were improved, the total effective rate was 89.28%;12 cases in control group were cured, 10 were effective, 2 were improved, 4 were ineffective, the total efficiency was 78.57%;the clini-cal curative effect of observation group was higher than control group (P<0.05). Conclusion:The clinical efficacy of traditional Chi-nese medicine combined with moxifloxacin in the treatment of senile pneumonia is significantly improved, which is worthy of popu-larization and application.

  3. Greatest International ANtiinfective Trial (GIANT with moxifloxacin in the treatment of acute exacerbation of  chronic bronchitis: subanalysis of Chinese data of a global, multicenter, noninterventional study

    Directory of Open Access Journals (Sweden)

    Yulin Feng

    2010-03-01

    Full Text Available Yulin Feng1, Faguang Jin2, Shuang Mu3, Hong Shen4, Xiaohong Yang5, Yuling Wang6, Zhenshan Wang7, Yingjun Kong8, Zuke Xiao9, Qiming Feng101Respiratory Department, West China Hospital, Sichuan University, China; 2Respiratory Department, Tang Du Hospital, China; 3Respiratory Department, Peking University People’s Hospital, China; 4Emergency Department, The General Hospital of the People’s Liberation Army, China; 5Respiratory Department, Xinjiang People’s Hospital, China; 6Respiratory Department, Shi Jiazhuang First Hospital, China; 7Respiratory Department, The Second Affiliated Hospital of Dalian Medical University, China; 8Respiratory Department, The First Clinical College of Harbin Medical University, China; 9Respiratory Department, The Jiangxi Provincial People’s Hospital, China; 10Emergency Department, Shanghai Sixth People’s Hospital, ChinaBackground and objective: A single infective acute exacerbation of chronic bronchitis (AECB has a sustained effect on health status. Although a number of clinical investigations have demonstrated the efficacy of antibiotics in AECB, increased bacterial resistance has caused concern about the efficacy of currently available antibiotic therapies. This subanalysis of a global noninterventional study aimed to evaluate the impact of AECB on the patient and the community and the effectiveness and safety of a treatment with moxifloxacin (MXF tablets in daily life clinical practice in China.Methods: This prospective, noninterventional, noncontrolled, multicenter observational study, which started in China in April 2004 and ended in February 2007, was part of the global GIANT study. Patients with a diagnosis of mild to severe AECB were treated with MXF tablets 400 mg for a period at the physician’s discretion. The observation period for each patient covered a complete treatment period with MXF. For each patient, the physician documented data at an initial visit (baseline and at least one follow-up visit

  4. Determination of moxifloxacin hydrochloridum standard by non-aqueous potentiometry titration%非水电位滴定法测定盐酸莫西沙星含量的方法研究

    Institute of Scientific and Technical Information of China (English)

    聂忠莉; 李长生; 唐雪枫; 陈小宁; 李芳

    2013-01-01

    目的:探究非水电位滴定法[李发美.分析化学[M].第6版.北京:人民卫生出版社,2007:152-179.]测定盐酸莫西沙星含量的方法。方法以0.01 mol/L盐酸溶液-无水甲醇(1∶8)为溶剂,用甲醇钠滴定液(0.1 mol/L)进行滴定。结果非水电位滴定法:方法精密度RSD为0.46%(n=9),平均回收率为100.0%。结论非水电位滴定法的测定结果与高效液相色谱法在统计学上无显著性差异,方法简便快速,结果准确可靠。%Objective To establish a non-aqueous potentiometry titration method for the content determination of moxifloxacin hydrochloridum standard. Methods 0.01 mol·L-1 solution of hydrochloric acid-anhydrous methanol(1:8) were used as solvent for non-aqueous potentiomerry titration. 0.1 mol·L-1 solution of sodium methylate was used to titrate the content of moxifloxacin hydrochloridum standard.Results Non-aqueous Potentiometry titration: The precision RSD was 0.46%(n=9), the average recovery was 100.0%. Conclusion Non-aqueous potentimetry titration method is identical statistically. Non-aqueous potentiometry titration method is accurate and used widely.

  5. 盐酸莫西沙星氯化钠注射液与氟康唑注射液的配伍稳定性考察%Study on Compatible Stability of Moxifloxacin Hydrochloride and Sodium Chlorid Injection Mixed with Fluconazole Injection

    Institute of Scientific and Technical Information of China (English)

    李俊; 张丽梅

    2011-01-01

    目的 考察盐酸莫西沙星氯化钠注射液与氟康唑注射液的配伍稳定性.方法 分别观察配伍液在室温(20±1)℃下放置8 h内的外观并测定pH变化,用紫外分光光度法测定主药含量.结果 两药配伍后4 h内,外观、pH、含量均无明显变化.结论 盐酸莫西沙星氯化钠注射液与氟康唑注射液在4 h内可以配伍使用.%Objective To observe the compatibility of Moxifloxacin Hydrochloride and sodium Chloride Injection with Fluconazole Injec-tion. Methods The change in appearance and pH value of the compatible solution placed at room temperature (20 ± 1) ℃ for 8 h was ob-served, while the content of moxifloxacin and fluconazole was determined by UV spectrophotometry as well. Results There were no signifi-cant changes in appearance, pH value and concentration after the mixture of the two drugs within 4 h. Conclusion Moxifloxacin Hydrochlo-ride and Sodium Chloride Injection can be mixed with Fluconazole Injection for use within 4 h.

  6. 利福布汀联合莫西沙星治疗耐多药肺结核的近期疗效观察%Short-term Therapeutic Effect of Rifabutin and Moxifloxacin on Multidrug Resistant Pulmonary Tuberculosis

    Institute of Scientific and Technical Information of China (English)

    张玲莉; 郭胜利; 翟守恒; 杨变转; 原艳明

    2013-01-01

    Objective:To evaluate the therapeutic effect of rifabutin(B)and moxifloxacin(M)on multi-drug resistant pulmonary tuberculosis (MDR-PTB).Method:41 cases of MDR-PTB were randomly divided into two qroups:21 cases of treatment group and 20 cases of control group.The treatment group were treated with moxifloxacin(M),rifabutin(B),associated with pasiniazid(D),protionamide(Th),amikacin(A),ethambutol (E).The control group were treated with levofloxacin(V),rifampicin(L),associated with pasiniazid(D),protionamide(Th),amikacin(A), ethambutol(E).The treatment course were all 18 months.Result:The sputum smear negative conversion rate was 85.7%(18/21)and 55.0%(11/20) respectively in the treatment group and control group(P0.05).The effective rate in chest X-ray was 81%(17/21),50%(10/20)respectively in the treatment group and the control group(P0.05).There were no significant differences in side effects between the groups(P>0.05).Conclusion:Regimens containing rifabutin and moxifloxacin are very effective in sputum negative conversion rate,lesion absorption and cavity closing for the treatment of multi-drug resistant pulmonary tuberculosis,with good safety and tolerance.%目的:评价利福布汀和莫西沙星联合化疗方案对耐多药肺结核(MDR-PTB)的疗效。方法:将41例MDR-PTB患者随机分为两组,治疗组21例,采用以莫西沙星(M)、利福布汀(B)为主,联合力克肺疾(D)、丙硫异烟胺(Th)、阿米卡星(A)、乙胺丁醇(E)治疗;对照组20例,采用以左氧氟沙星(V)、利福喷汀(L)为主,联合D、Th、A、E治疗;疗程均为18个月。结果:治疗组痰菌阴转率为85.7%(18/21),对照组痰菌阴转率为55.0%(11/20),治疗组明显高于对照组(P0.05)。治疗组和对照组病灶有效率分别为81.0%(17/21)、50.0%(10/20),差异有统计学意义(P0.05),且两组间副作用比较差异无统计学意义(P>0.05)。结论:利福布汀联

  7. QT interval prolongation and type I hypersensitivity reaction caused by orally taken moxifloxacin%莫西沙星片致 QT 间期延长和速发型变态反应

    Institute of Scientific and Technical Information of China (English)

    欧登科; 周晓明; 陈愉; 肇丽梅; 赵立

    2014-01-01

    Mezlocillin sodium and sulbactam sodium for injection(2. 5 g,once every 12 hours)and azithromycin injection(0. 5 g,once daily)were given to a 23-year-old female patient due to the community-aquired mycoplasma pneumonia and acute purulent tonsillitis. On the eleventh day,the treatments above mentioned were changed to oral moxifloxacin 0. 4 g once daily because of the improvement of the patient's condition. Thirty minutes after the moxifloxacin administration for the first time,the patient felt chest tightness,palpitation,fever,and generalized flush accompanied by wheal and papula on her limbs and trunk. The electrocardiogram examination showed double directional or inverted T waves in the precordial leads and prolonged QT period with corrected QT interval 462 ms. Laboratory examination showed creatine kinase(CK)239 U/ L,isoenzyme of creatine kinase(CK-MB)35 U/ L. TypeⅠhypersensitivity reaction was diagnosed. Moxifloxacin was stopped and ananaphylaxis and supportive treatments were given immediately. Three days later,her skin symptoms disappeared,ECG examination result returned to normal. The levels of CK and CK-MB were 40 U/ L and 11 U/ L,respectively.%1例23岁女性患者因支原体肺炎合并急性化脓性扁桃体炎给予注射用美洛西林钠舒巴坦钠(2.5 g,1次/12 h)和阿奇霉素(0.5 g,1次/ d)静脉滴注,第11天因病情好转改为莫西沙星0.4 g口服,1次/ d。患者首次服用莫西沙星后约30 min 出现胸闷、心悸、气促、发热,接着出现颜面及全身潮红,四肢及躯干出现风团、红色丘疹伴瘙痒,心电图提示胸前导联 T 波双向、倒置,QT 间期延长,校正的 QT 间期为462 ms。实验室检查示肌酸激酶(CK)239 U/ L,CK 同工酶(CK-MB)35 U/ L。诊断为速发型过敏反应。停用莫西沙星并立即给予抗过敏治疗及对症处理,3 d 后皮肤症状消失,心电图正常,CK 40 U/ L,CK-MB 11 U/ L。

  8. 莫西沙星片致 QT 间期延长和速发型变态反应%QT interval prolongation and type I hypersensitivity reaction caused by orally taken moxifloxacin

    Institute of Scientific and Technical Information of China (English)

    欧登科; 周晓明; 陈愉; 肇丽梅; 赵立

    2014-01-01

    1例23岁女性患者因支原体肺炎合并急性化脓性扁桃体炎给予注射用美洛西林钠舒巴坦钠(2.5 g,1次/12 h)和阿奇霉素(0.5 g,1次/ d)静脉滴注,第11天因病情好转改为莫西沙星0.4 g口服,1次/ d。患者首次服用莫西沙星后约30 min 出现胸闷、心悸、气促、发热,接着出现颜面及全身潮红,四肢及躯干出现风团、红色丘疹伴瘙痒,心电图提示胸前导联 T 波双向、倒置,QT 间期延长,校正的 QT 间期为462 ms。实验室检查示肌酸激酶(CK)239 U/ L,CK 同工酶(CK-MB)35 U/ L。诊断为速发型过敏反应。停用莫西沙星并立即给予抗过敏治疗及对症处理,3 d 后皮肤症状消失,心电图正常,CK 40 U/ L,CK-MB 11 U/ L。%Mezlocillin sodium and sulbactam sodium for injection(2. 5 g,once every 12 hours)and azithromycin injection(0. 5 g,once daily)were given to a 23-year-old female patient due to the community-aquired mycoplasma pneumonia and acute purulent tonsillitis. On the eleventh day,the treatments above mentioned were changed to oral moxifloxacin 0. 4 g once daily because of the improvement of the patient's condition. Thirty minutes after the moxifloxacin administration for the first time,the patient felt chest tightness,palpitation,fever,and generalized flush accompanied by wheal and papula on her limbs and trunk. The electrocardiogram examination showed double directional or inverted T waves in the precordial leads and prolonged QT period with corrected QT interval 462 ms. Laboratory examination showed creatine kinase(CK)239 U/ L,isoenzyme of creatine kinase(CK-MB)35 U/ L. TypeⅠhypersensitivity reaction was diagnosed. Moxifloxacin was stopped and ananaphylaxis and supportive treatments were given immediately. Three days later,her skin symptoms disappeared,ECG examination result returned to normal. The levels of CK and CK-MB were 40 U/ L and 11 U/ L,respectively.

  9. Efficacy of amoxicillin and clavulanate in combination with moxifloxacin and esomeprazole in treatment of drug resistant Helicobacter pylor infection%四联疗法治疗耐药幽门螺杆菌感染的疗效分析

    Institute of Scientific and Technical Information of China (English)

    李仁祥; 杨英君; 卢惠伦

    2013-01-01

    Objective To observe the efficacy of amoxicillin and clavulanate potassium combined with moxifloxacin and esomeprazole in treatment of Helicobactor pylori infection failed after treatment with conventional triple therapy for the treatment of failure of drug resistance of Helicobacter pylori infection,and a control group with quadruple therapy for Helicobacter pylori eradication rate difference.Methods The 85 patients with Helicobacter pylori infections and treated with conventional triple therapy for a course of over 2 weeks and confirmed treatment failure by the C14 breath test were randomly divided into therapeutic group and control group.The patients in the therapeutic group were treated with amoxicillin potassium clavulanate 914mg Bid+ moxifloxacin tablet 400mg Qd+ esomeprazole 20mg Bid.Those in the control group were treated with esomeprazole 20mg Bid+ colloidal bismuth tartrate 165mg Qid+ amoxicillin capsule 1000mg Bid + clarithromycin 500mg Bid,for a course of 1 week.Results About 83.3% of Helicobacter pylori in the patients of therapeutic group was eradicated and eradication rate in the control group was 70% showing significant difference(P<0.05).Conclusions Combined use of amoxicillin and clavulanate potassium,moxifloxacin and esomeprazole is superior to the quadruple therapy containing bismuth in treatment of Helicobactor pylori-infected patients failed with conventional triple therapy.The regimen could be used as the second-line therapy with mild side effects.%目的 观察阿莫西林克拉维酸钾和莫西沙星与埃索美拉唑联用,治疗传统的三联疗法治疗失败的耐药幽门螺杆菌感染的根除率,并与对照组四联疗法比较是否存在差异. 方法 将85例传统的三联疗法治疗2周以上,经C14呼气试验检查证实治疗失败的耐药幽门螺杆菌感染病例,随机分为试验组及对照组,试验组治疗方案为阿莫西林克拉维酸钾片914mgBid+莫西沙星片400mgQd+埃索美拉唑片20mgBid,对

  10. 联合应用莫西沙星和微卡方案治疗耐多药肺结核病的临床研究%Clinical Research on Combined Application of Moxifloxacin and Micro Card Scheme in the Treatment of Multidrug-resistant ;Tuberculosis

    Institute of Scientific and Technical Information of China (English)

    吴斌; 朱丽阳

    2014-01-01

    目的:研究联合应用莫西沙星和微卡方案治疗耐多药肺结核病的临床疗效。方法:选取2010年8月-2013年8月在本院接受治疗的78例耐多药肺结核患者作为研究对象,将所有患者按照随机数字表法分为莫西沙星治疗组、微卡治疗组、联合治疗组各26例。三组患者均进行常规结核病化疗,莫西沙星治疗组使用莫西沙星等药物进行治疗,微卡治疗组使用微卡等方法进行治疗,联合治疗组应用莫西沙星组与微卡治疗组的治疗方法,观察比较细菌学检查及X线检查结果。结果:联合治疗组治疗540 d痰菌阴转率明显高于莫西沙星治疗组和微卡治疗组,且治疗结束后痰菌培养阳性率明显低于莫西沙星治疗组和微卡治疗组,差异均有统计学意义(P0.05)。结论:联合应用莫西沙星和微卡方案治疗耐多药肺结核病临床疗效显著,但存在一定的不良反应。%Objective:To study the clinical curative effect on combined application of moxifloxacin and micro card scheme in the treatment of multi-drug resistant tuberculosis.Method:78 multi-drug resistant tuberculosis patients were selected from August 2010 to August 2013 in our hospital for treatment as research objects,they were divided into the moxifloxacin treatment group,the micro card treatment group and the combination group according to random number table method,26 cases in each group.Three groups of patients were treated with routine TB chemotherapy,the moxifloxacin treatment group was given the length magnitude drug treatment,the micro card treatment group was given micro card treatment methods,the combination group was given moxifloxacin treatment combined with micro card treatment,bacteriology inspection and X-ray inspection result of three groups were observed and compared.Result:The 540 d phlegm germ feminine changed rate of the combination group was significantly higher than that of moxifloxacin treatment

  11. 口服莫西沙星致过敏伴腹痛和阴道出血%Allergy complicated with abdominal pain and colporrhagia due to oral moxifloxacin

    Institute of Scientific and Technical Information of China (English)

    姚丹林; 牟红梅

    2015-01-01

    1例45岁女性患者因急性支气管炎自行口服莫西沙星0.4 g。约5 min 后,患者出现周身瘙痒、全身皮肤发红、颜面水肿、声音嘶哑、呕吐、腹痛、腹泻;约25 min 后出现视物模糊、神志恍惚。遂静脉注射甲泼尼龙120 mg,快速静脉滴注0.9%氯化钠注射液1000 ml,但患者腹痛无缓解,服药后1 h 出现阴道出血。腹部超声检查未见明显异常。给予哌替啶100 mg 肌内注射,约30 min 后患者腹痛减轻,口服莫西沙星24 h 后阴道出血停止。患者1个月前有相同发病及诊治经历。%A 45 year-old woman with acute bronchitis took moxifloxacin 0. 4 g orally by herself. She developed pruritus and rubefaction on the whole body,edema in the face,hoarseness,vomiting,abdominal pain,and diarrhea 5 minutes after taking the medicine. About 25 minutes later,she developed clouded vision and became unconscious. She was given an intravenous injection of methylprednisolone 120 mg,and rapid intravenous drip of 0. 9% sodium chloride injection 1 000 ml. But the abdominal pain did not alleviate. She developed colporrhagia one hour after taking the medicine. The result of abdominal ultrasound examination showed no obvious abnormity. Then she was given an intramuscular injection of meperidine 100 mg. About 30 minutes later,her abdominal pain was relieved. Twenty-four hours after receiving moxifloxacin,her colporrhagia was stopped. The patient had a similar experience of morbidity,diagnosis and therapy one month ago.

  12. Analysis of 1416 Cases of Moxifloxacin-inducing Adverse Drug Reaction/event in Beijing Area from 2007 to 2011%北京地区2007~2011年1416例莫西沙星不良反应/事件分析

    Institute of Scientific and Technical Information of China (English)

    曾艳; 闫素英

    2013-01-01

    Objective:To analyze the reports of moxifloxacin-inducing adverse drug reaction/event(ADR/ADE)in Beijing area to provide reference for rational use of moxifloxacin.Methods:Collect reports of ADR/ADE induced by moxifloxacin received by the national drug adverse reaction monitoring center from January 1 th in 2007 to December 31 th in 2011,classify the adverse reactions according to MedDRA and make analysis of the patients'clinical characteristics and type and outcome of the ADR/ADE.Results:Off-label use of moxifloxacin was prominent.The clinical manifestations of ADR/ ADE were mostly skin and subcutaneous tissue damages,nervous system disorders and gastrointestinal disorders.And the serious ADR/ADE were mostly immune system disorders,nervous system disorders and hepatobiliary disorders.Conclusion:Safe to use with moxifloxacin is worth paying attention by clinicians,who should detect and deal with ADR/ADE of moxifloxacin in time on baisis of rational use.%目的:统计分析北京地区近5年来莫西沙星不良反应/事件报告,为莫西沙星的安全合理应用提供依据.方法:收集整理2007年1月1日~2011年12月3日全国药品不良反应监测网络接收到的北京地区怀疑药品为莫西沙星的不良反应/事件报告,提取患者一般情况、用药情况、不良反应/事件发生情况及转归等信息,按照MedDRA系统将不良反应或不良事件进行系统分类,进行统计分析.结果:莫西沙星的不良反应/事件报告中,患者超说明书用药、超适应证用药较为突出,不良反应主要表现在皮下及皮下组织症状、神经系统症状、胃肠系统症状等.而严重不良反应以免疫系统症状、神经系统症状、肝胆系统症状为主.结论:莫西沙星的安全性是临床应关注的问题,应该在合理使用的基础上,监测并及时处理其不良反应/事件.

  13. HPLC-荧光法测定莫西沙星在犬体内的血药浓度及其药动学研究%Determination of Plasma Concentration of Moxifloxacin in Beagle Dogs by HPLC-fluorescent Method and Its Pharmacokinetic Study

    Institute of Scientific and Technical Information of China (English)

    徐建平; 易红; 袁浩宇

    2012-01-01

    目的:建立测定犬血浆中莫西沙星浓度的方法,并研究其药动学特性.方法:取Beagle犬6只,单剂量灌服莫西沙星120mg,采用高效液相色谱-荧光法测定给药后72 h内血浆中莫西沙星的浓度,采用DAS软件计算药动学参数.色谱柱为HypersilBDSC18,流动相为乙腈-1.0mol·L-1醋酸溶液(25∶75,pH=2.0),流速为1.0 mL· min-,激发波长为273 nm,发射波长为488 nm.结果:莫西沙星检测浓度的线性范围为0.1~20.0 μg·mL-1(r=0.999 2),平均相对回收率为89.5%~96.7%,日内和日间RSD均<6%.莫西沙星在犬体内的药动学特性符合二室模型,主要药动学参数Cmax:( 10.25±4.21) μg·mL-1,tmax:(3.0±1.0)h,t1/2β:(9.76±2.48)h,AUC0~24h:(142.10±49.80) mg·h·L-1.结论:本方法准确、灵敏、快速,适用于莫西沙星的血药浓度测定和药动学研究.%OBJECTIVE: To establish the method for determining the plasma concentration of moxifloxacin in Beagle dogs, and to study pharmacokinetic characteristics of it. METHODS: 6 Beagle dogs were given a single dose of moxifloxacin 120 mg. The plasma concentration of moxifloxacin in samples within 72 h after administration was determined by HPLC-fluorescent method and pharmacokinetic parameters was calculated by using DAS software. The determination was performed on Hypersil BDS C18 column with mobile phase consisted of acetonitrile-1.0 mol·L‐1 acetic acid solution (25:75, pH=2.0) at the flow rate of 1.0 mL·min‐1. The excitation wavelength was set at 273 nm and emission wavelength was set at 488 run. RESULTS: The liner range of moxifloxacin was 0.1~20.0 μg·mL‐1 (r=0.999 2) with relative recovery rate of 89.5%~96.7%. The RSDs of intra-day and inter-day were less than 6%. The pharmacokinetics of moxifoxacin was fitted to two-compartment open pharmacokinetic model. The main pharmacokinetic parameters of moxifloxacin were as follows: Cmax: (10.25 ±4.21) μg·mL‐1, tmax: (3.0± 1.0) h, t1/2β: (9.76±2.48) h, AUCo~24h

  14. Pharmacokinetics and Pharmacodynamics of Moxifloxacin in the Treatment of Lower Respiratory Tract Infection%盐酸莫西沙星氯化钠注射液治疗下呼吸道感染的药动学和药效学研究

    Institute of Scientific and Technical Information of China (English)

    朱文静; 王少华; 张淑立; 赵艳; 张文竹; 孙韬华

    2013-01-01

    目的:研究盐酸莫西沙星氯化钠注射液在下呼吸道感染患者体内的药动学、临床疗效及病原学疗效.方法:选取符合条件的下呼吸道感染患者18例,给予莫西沙星氯化钠注射液400 mg/d静脉滴注.采用高效液相色谱法测定莫西沙星的血药浓度,观察并记录临床及病原学疗效.在治疗前、后留取患者的痰标本进行细菌培养,采用肉汤稀释法进行细菌最低抑菌浓度(MIC)的测定.结果:莫西沙星的峰浓度、谷浓度、稳态血药浓度分别为(3.69±0.05)、(1.43±0.10)、(4.25±0.06)mg/L.经莫西沙星氯化钠注射液治疗的18例下呼吸道感染患者,15例治愈,1例显效,2例进步,总有效率达到88.89%.临床各项症状体征大多在用药3d后得到明显改善.病原菌清除率为88.89%.结论:盐酸莫西沙星氯化钠注射液在治疗下呼吸道感染时可以达到有效的治疗浓度,并取得良好的治疗效果.%OBJECTIVE:To study the pharmacokinetics,clinical and bacteriological efficacies of moxifloxacin in the treatment of lower respiratory tract infection.METHODS:18 cases of lower respiratory tract infection were collected and treated with moxifloxacin 400 mg/d intravenously.The blood concentration of moxifloxacin was determined by HPLC.Both clinical and bacteriological efficacies were both recorded.The sputum specimens collected before and after the therapy were cultured to measure the MIC by broth dilution method.RESULTS:The peak concentration,trough concentration and steady state blood concentration of moxifloxacin reached (3.69 ± 0.05)mg/L,(1.43 ± 0.10)mg/L and (4.25 ± 0.06)mg/L,respectively.Among 18 cases of lower respiratory tract infection treated with Moxifloxacin sodium chloride injection,15 cases were cured,1 case was significantly effective and 2 cases improved.The total effective rate was 88.89%.Most of the clinical symptoms and signs improved obviously after 3 days.Bacteria clearance was 88.89

  15. Characteristics associated with non-participation in 7-day accelerometry

    Directory of Open Access Journals (Sweden)

    Franziska Weymar

    2015-01-01

    Conclusion: We found a self-selection bias in participation in accelerometry. Women declined study participation more likely than men. The number of cardiometabolic risk factors decreased compliance only in men. Future studies should consider strategies to reduce this bias.

  16. Mortality after surgery in Europe: a 7 day cohort study

    NARCIS (Netherlands)

    Pearse, R.M.; Moreno, R.P.; Bauer, P.; Pelosi, P.; Metnitz, P.; Spies, C.; Vallet, B.; Vincent, J.L.; Hoeft, A.; Rhodes, A.; Pickkers, P.; Bouw, M.P.

    2012-01-01

    BACKGROUND: Clinical outcomes after major surgery are poorly described at the national level. Evidence of heterogeneity between hospitals and health-care systems suggests potential to improve care for patients but this potential remains unconfirmed. The European Surgical Outcomes Study was an intern

  17. Clinical Effect of Moxifloxacin or Levofloxacin in the Treatment of Multi Drug Resistant Pulmonary Tuberculosis%莫西沙星或左氧氟沙星用于耐多药肺结核治疗中的临床效果

    Institute of Scientific and Technical Information of China (English)

    李莹莹

    2016-01-01

    目的:研究莫西沙星或左氧氟沙星治疗耐多药肺结核的临床效果。方法选取我院2013年12月~2014年12月收治的耐多药肺结核病患者62例,分为两组,A组用莫西沙星治疗,B组用左氧氟沙星治疗,分析两组治疗效果。结果 A组治疗后的总有效率高于B组(P<0.05);两组不良反应比较差异无统计学意义(P>0.05)。结论采用莫西沙星治疗耐多药肺结核的效果突出。%Objective To study moxifloxacin or levofloxacin for clinical effect in the treatment of multi drug resistant pulmonary tuberculosis. MethodsOur hospital were treated multi-drug resistant tuberculosis patients 62 cases between December 2013 and December 2014. They were divided into two groups,group A was treated with moxifloxacin,group B was treated with levofloxacin,analysis of two groups of therapeutic effect.ResultsAfter treatment the total effective rate of group A was higher than group B(P0.05.Conclusion Using moxifloxacin in treatment of multi-drug resistant tuberculosis,the effect is remarkable.

  18. In vitro antibiotic susceptibilities of ocular bacteria isolates from the cornea and conjunctiva to moxifloxacin, gatifloxacin and other fluoroquinolones Testes de sensibilidade antimicrobiana de bactérias isoladas da córnea e da conjuntiva à moxifloxacina, gatifloxacina e outras fluoroquinolonas

    Directory of Open Access Journals (Sweden)

    Ana Luisa Höfling-Lima

    2004-12-01

    Full Text Available PURPOSE: To assess and compare the in vitro susceptibility of ocular bacterial isolates to fluoroquinolones, including moxifloxacin and gatifloxacin. METHODS: A total of 154 bacterial ocular isolates from keratitis and conjunctivitis were tested for sensitivity to the studied antibiotics, using the disk diffusion method. RESULTS: Of the 51 corneal isolates, 46 (90.2% were sensitive to ciprofloxacin, 45 (88.2% to ofloxacin and 41 (80.4% to lomefloxacin. All corneal isolates were sensitive to moxifloxacin and gatifloxacin. Of the 103 bacterial conjunctival isolates, 101 (98.1% and 103 (100% were sensitive to gatifloxacin and moxifloxacin, respectively. Two strains of Streptococcus sp viridans group were resistant to gatifloxacin. A total of 82 (79.6% specimens were sensitive to lomefloxacin, 89 (86.4% and 96 (93.2% to ciprofloxacin and ofloxacin, respectively. CONCLUSION: Fourth generation fluoroquinolones, herein exemplified by moxifloxacin and gatifloxacin, seem to be more effective than previous generation fluoroquinolones against frequently encountered organisms isolated from patients with bacterial keratitis and conjunctivitis.OBJETIVOS: Conhecer e comparar a susceptibilidade in vitro de bactérias isoladas do olho à fluoroquinolonas, incluindo moxifloxacina e gatifloxacina. MÉTODOS: Um total de 154 bactérias isoladas de ceratites e conjuntivites foram submetidas a testes de sensibilidade antimicrobiana aos antibióticos usando o método de difusão em disco. RESULTADOS: Das 51 bactérias isoladas da córnea, 46 (90,2% foram sensíveis a ciprofloxacina, 45 (88,2% a ofloxacina e 41 (80,4% a lomefloxacina. Todas as bactérias isoladas da córnea foram sensíveis a moxifloxacina e a gatifloxacina. Das 103 bactérias isoladas da conjuntiva 101 (98,1% e 103 (100% foram sensíveis a gatifloxacina e moxifloxacina, respectivamente. Duas cepas de Streptococcus sp do grupo viridans mostraram-se resistentes à gatifloxacina. Um total de 82 (79,6% esp

  19. Clinical comparative observation and its pharmacoeconomic evaluation of moxifloxacin vs. cefoperazone sodium and tazobactam sodium on genitourinary infection%莫西沙星与头孢哌酮钠/他唑巴坦钠用于泌尿生殖系感染的临床对比观察及其药物经济学评价

    Institute of Scientific and Technical Information of China (English)

    李爱国

    2013-01-01

    目的 比较莫西沙星与头孢哌酮钠/他唑巴坦钠治疗泌尿生殖系感染的临床疗效,并通过药物经济学分析,为临床合理选用抗菌药物提供参考依据.方法 选择115例泌尿生殖系感染患者,按随机数字表法分为莫西沙星组(59例)和头孢哌酮钠/他唑巴坦钠组(头孢组,56例),疗程均为7d.比较两组的临床疗效及不良反应发生率,对两种药物进行药物经济学评价.结果 莫西沙星组与头孢组临床总有效率分别为86.4%(51/59)、85.7%(48/56),细菌学总清除率分别为89.3%(67/75)、88.9%(64/72),两组比较差异均无统计学意义(P>0.05).莫西沙星组与头孢组的增量成本-效果比分别为2.29、21.56,差异有统计学意义(x2=271.126,P<0.01).莫西沙星组与头孢组的不良反应发生率分别为6.8%(4/59)、3.6%(2/56),差异无统计学意义(x2=0.598,P> 0.05).结论 在泌尿生殖系感染的治疗中,莫西沙星与头孢哌酮钠/他唑巴坦钠疗效相当,但莫西沙星能更好地减少治疗成本,服用方便,是治疗泌尿生殖系感染较好的治疗方案.%Objective To compare the clinical efficacy of moxifloxacin vs.cefoperazone sodium/tazobactam sodium in the treatment of genitourinary infection,and provide a reference for clinical rational use of antimicrobial drugs through pharmacoeconomics studies.Methods A total of 115 cases with genitourinary infection were randomly divided into moxifloxacin group (59 cases) and cefoperazone sodium/tazobactam sodium group (cephalosporin group,56 cases) by random digits table method.Moxifloxacin group was treated with moxifloxacin hydrochloride tablets,cephalosporin group was given cefoperazone sodium/tazobactam sodium by intravenous infusion for 7 d.The clinical efficacy and incidence of adverse reaction was compared,and the two drugs were evaluated by pharmacoeconomic study.Results The clinical total effective rate in moxifloxacin group and cephalosporin group was respectively

  20. 莫西沙星和左氧氟沙星在耐多药肺结核病治疗方案中的效果比较%Comparison of Clinical Effect of Moxifloxacin and Levofloxacin in treatment of Multi-Drug Resistant Pulmonary Tuberculosis

    Institute of Scientific and Technical Information of China (English)

    邓敏

    2015-01-01

    目的:临床使用莫西沙星及左氧氟沙星治疗耐多药肺结核病,比较其临床作用效果。方法选取60例2012年9月—2014年10月该所经确诊为耐多药肺结核病患者临床资料进行回顾性分析,根据用药原则不同分成左氧氟沙星组与莫西沙星组,每组各为30例。两组均进行统一基础抗结核方案治疗,并各自加用所选择药物。分别对两组临床治疗效果进行观察并作比较。结果莫西沙星组整体治疗有效率为93.33%,左氧氟沙星组整体治疗有效率为80.00%,比较结果显示莫西沙星组治疗效果更为显著,P0.05。结论莫西沙星治疗耐多药肺结核效果显著,引起的不良反应较少,临床可推广应用。%Objective To compare the clinical effects of moxifloxacin and levofloxacin in the treatment of multi-drug resistant pul-monary tuberculosis. Methods The clinical data of 60 patients with multi-drug resistant pulmonary tuberculosis confirmed in our hospital from September 2012 to October 2014 was retrospectively reviewed. The patients were divided into levofloxacin group and moxifloxacin group according to different medication principles, 30 cases in each group. Two groups adopted the unified basic treatment and separately chose the different drugs. The clinical treatment effect of two groups was observed and compared. Results The total effective rate of the moxifloxacin group (93.33%) was significantly higher than that of levofloxacin group (80.00%) ( P0.05). Conclusion Moxifloxacin has an obvious treatment effect in multi-drug resistant pulmonary tuberculosis and fewer adverse reactions. It is wor-thy of clinical promotion and application.

  1. 连锁酒店产品战略分析--以济南市槐荫区7天连锁酒店为例%Analysis of chain hotel product strategy:Taking Ji'nan District 7 Days Inn as an example

    Institute of Scientific and Technical Information of China (English)

    曹炜

    2013-01-01

      以济南市槐荫区7天连锁酒店经十中路分店的产品销售战略为研究对象,主要采用随机问卷调查和实地考察的方法进行数据收集,运用 Excel软件对调查的数据进行整理分析,以SWO T战略组合模型提出该酒店可以采用WO 战略,即扭转内部劣势把握外部机会,使其产品在同类酒店中更具优势,从而使得7天连锁酒店在济南市经济型酒店市场中更具竞争力。%In Huaiyin District of Ji'nan city 7 Days Inn after ten road store sales strategy as the research ob-ject ,mainly adopts the method of random questionnaire and on-the-spot investigation to collect data ,use Excel software to analyze the survey data ,the SWOT strategic combination model is proposed for the hotel can adopt the WO strategy ,namely the torsion internal weaknesses grasp the external opportunities ,make the products more competitive in the same hotel ,w hich makes the 7 Days Inn in the more competitive mar-ket in Ji'nan city Econo Hotel .

  2. Clinical observation of moxifloxacin in the treatment of acute exlosion chronic obstructive pulmonary disease patients with invasive mechanical ventilation%莫西沙星治疗慢性阻塞性肺疾病急性加重期有创机械通气患者的临床效果观察

    Institute of Scientific and Technical Information of China (English)

    林钦汉; 罗刚; 陈军

    2014-01-01

    目的 观察莫西沙星治疗慢性阻塞性肺疾病急性加重期(AECOPD)有创机械通气患者的临床效果.方法 将60例AECOPD有创机械通气患者完全随机分成观察组和对照组,各30例.莫西沙星组静脉滴注莫西沙星治疗0.4g,1次/d,治疗7~ 14 d;左氧氟沙星组静脉滴注左氧氟沙星,0.5g,1次/d,治疗7~14d.比较2组疗效、细菌清除率及脱呼吸机时间.结果 莫西沙星组的临床有效率[83.3%(25/30)]、细菌清除率[86.7% (26/30)]、脱呼吸机成功率[76.7% (23/30)]均高于左氧氟沙星组[46.7%(14/30),53.3% (16/30),43.3% (13/30)](P<0.01或P<0.05),莫西沙星组低血糖发生率较左氧氟沙星组低[3.0% (1/30)比23.3% (7/30)],差异有统计学意义(P<0.01).结论 莫西沙星治疗AECOPD有创机械通气患者的临床疗效优于左氧氟沙星,且不良反应发生率低.%Objective To observe the clinical effects of moxifloxacin on acute exlosion chronic obstructive pulmonary disease(AECOPD) with invasive mechanical ventilation.Methods Sixty patients of AECOPD with invasive mechanical ventilation were randomly divided into two groups; they were treated with moxifloxacin and levofloxacin respectively.The efficacy rates,bacteriums eradication rates and the time detaching from the ventilator were compared.Results The efficacy rates (83.3%,25/30),bacteriums eradication rates (86.7%,26/30),the success rate of detaching from the ventilator (76.7%,23/30) in moxifloxacin group were higher than those in the levofloxacin group [46.7% (14/30),53.3% (16/30),43.3% (13/30)] (P < 0.01 or P < 0.05),the incidence of adverse drug reactions in moxifloxacin group lower than that in the levofloxacin group [3.0% (1/30) vs 23.3 % (7/30)],with statistical significance (P < 0.01).Conclusion In the treatment of AECOPD that need for invasive mechanical ventilation patients,moxifloxacin has good clinical efficacy.

  3. 莫西沙星联合卷曲霉素治疗耐多药肺结核的临床效果观察%Clinical effect of moxifloxacin combined with capreomycin in the treatment of multi-drug resistant tuberculo-sis

    Institute of Scientific and Technical Information of China (English)

    刘东华

    2014-01-01

    Objective To explore the clinical effect of moxifloxacin combined with capreomycin in the treat-ment of multi-drug resistant tuberculosis. Methods 108 patients with multi-drug resistant tuberculosis were random-ly divided into two groups, 54 cases in each group. The control group was treated with moxifloxacin, and the experi-ment group was treated with moxifloxacin combined with capreomycin. The clinical effect of the two groups was ob-served after the treatment. Results The total absorption rate of lesion was 88. 33% and the cavity closure rate was 85. 19% in the experiment group, which were significantly higher than those in the control group ( 57. 40% and 59. 62%) (P<0. 05). Conclusion The application of moxifloxacin combined with capreomycin is more effective than moxifloxacin alone in the treatment of patients with multi-drug resistant tuberculosis, which has higher cure rate and no obvious adverse reactions.%目的:对莫西沙星联合卷曲霉素治疗耐多药肺结核的临床效果进行探讨。方法选取108名在我院进行治疗的耐多药肺结核的患者,随机分成对照组和实验组,平均每组54人。对照组通过使用莫西沙星治疗耐多药肺结核,实验组则采用莫西沙星联合卷曲霉素治疗耐多药肺结核。比较并观察两组患者治疗后的临床效果。结果两组患者经过一段时间的治疗后,实验组的病灶总吸收率(88.33%)、空洞闭合有效率(85.19%)明显高于对照组的病灶总吸收率(57.40%)、空洞闭合有效率(59.62%),统计学上有意义(P<0.05)。结论通过观察分析使用莫西沙星联合卷曲霉素治疗耐多药肺结核的疗效明显好于单独使用莫西沙星的治疗效果,病灶吸收和空洞闭合明显高于对照组。治愈率高,无不良反应,安全可靠,值得在临床上广泛应用。

  4. 莫西沙星对多重耐药肺结核患者血清对氧磷酶活性及氧化应激指标影响研究%Effect of moxifloxacin on serum levels of PON1 and oxidative markers in patients with multiple drug resistant tuberculosis

    Institute of Scientific and Technical Information of China (English)

    杨宏生; 许锡荣; 沈琪; 金妍

    2016-01-01

    目的 探讨莫西沙星对多重耐药肺结核患者血清对氧磷酶(PON1)活性及氧化应激指标影响.方法 本研究连续性收录2012年1月至2014年6月于我院就诊并被确诊为耐多药肺结核的患者80例,分为莫西沙星组(n=40)和对照组(n=40).监测治疗前后血清总氧化态(TOS)、总抗氧化态(TAS)、氧化应激指数(OSI)和PON1水平改变.结果 莫西沙星组患者治疗后TAS和PON1水平明显上升(P<0.05),TOS和OSI水平明显下降(P<0.05),而对照组仅可见TOS和OSI水平明显下降(P<0.05).莫西沙星与△TAS(r=0.703,P=0.01)和△PON1(r=0.783,P=0.01)呈正相关,与△TOS(r=-0.598,P=0.03)呈负相关.结论 MDR-TB患者体内处于氧化应激损伤状态,通过莫西沙星联合抗痨治疗后可以明显改善氧化-抗氧系统的失衡情况.%Objective To evaluate the effect of moxifloxacin on serum levels of PON1 and oxidative markers in patients with multiple drug resistant tuberculosis(MDR-TB). Methods 80 patients with MDR-TB were enrolled in this study and divided into two group:the moxifloxacin group(n=40)and the control group(n=40)randomly and equally. The changes of serum levels of TAS, TOS, OSI and PON1 after treatment were recorded and analyzed. Results The levels of TAS and PON1 were significantly increased in the moxifloxacin group after anti-TB treatment, while the levels of TOS and OSI were significantly decreased. Correlation analysis showed that moxifloxacin adminis-tration was positively associated with changes of TAS and PON1, while negatively related with changes of TOS and OSI. Conclusion Oxidative stress injury exists in patients with MDR-TB and moxifloxacin treatment can improve the unbalance of oxidative and anti-oxidative system, which may contribute to enhance anti-tuberculosis therapeutic effect.

  5. A randomized, placebo-controlled, four-period crossover, definitive QT study of the effects of APF530 exposure, high-dose intravenous granisetron, and moxifloxacin on QTc prolongation

    International Nuclear Information System (INIS)

    Regulatory concern about potential QT-interval prolongation by serotonin-receptor antagonist antiemetics prompted product-label changes. The first-generation serotonin-receptor antagonist granisetron is available in oral (PO), intravenous (IV), and transdermal formulations. APF530 is a formulation that provides sustained release of granisetron when administered as a single subcutaneous (SC) injection. The Phase I study reported here evaluated effects of APF530 on electrocardiographic intervals. This single-site, double-blind, placebo-controlled, four-period crossover trial randomized healthy men and women to receive varying sequences of APF530 1 g SC, granisetron 50 μg/kg IV, moxifloxacin 400 mg PO, and placebo. Subjects were assessed for 49 hours after each treatment. The primary objective was to evaluate differences between baseline-adjusted, heart rate-corrected QT-interval change using the Fridericia rate correction (dQTcF) for APF530 1 g SC and placebo. Electrocardiograms were performed at various times throughout the assessment period. Pharmacokinetics and safety were evaluated. The upper one-sided 95% confidence interval (CI) for mean baseline-adjusted dQTcF at each post-dose time point between APF530 and placebo excluded 10 ms, indicating that APF530 1 g SC had no clinically significant effect on QTcF. Maximum observed QTcF change was 4.15 ms (90% CI, 0.94 to 7.36) at Hour 3. No clinically significant changes in other electrocardiogram intervals were observed. APF530 SC pharmacokinetics were as expected, with slow absorption (maximum plasma concentration 35.8 ng/mL, median time to maximum plasma concentration 11.1 hours) and slow elimination (mean half-life 18.6 hours; systemic clearance 20.2 L/hour) of granisetron versus the expected early peak concentration and elimination of granisetron IV. APF530 SC was well tolerated. Adverse events, most commonly constipation and SC injection-site reactions, were generally mild and quickly resolved. APF530 1 g SC did

  6. A randomized, placebo-controlled, four-period crossover, definitive QT study of the effects of APF530 exposure, high-dose intravenous granisetron, and moxifloxacin on QTc prolongation

    Directory of Open Access Journals (Sweden)

    Mason JW

    2014-03-01

    Full Text Available Jay W Mason,1 Thomas E Moon,2 Erin O’Boyle,3 Albert Dietz41Department of Medicine, University of Utah, Salt Lake City, UT, 2Tarizona eHealth Services, Inc., San Carlos, CA, 3AP Pharma, Redwood City, CA, 4Spaulding Clinical Research, West Bend, WI, USABackground: Regulatory concern about potential QT-interval prolongation by serotonin-receptor antagonist antiemetics prompted product-label changes. The first-generation serotonin-receptor antagonist granisetron is available in oral (PO, intravenous (IV, and transdermal formulations. APF530 is a formulation that provides sustained release of granisetron when administered as a single subcutaneous (SC injection. The Phase I study reported here evaluated effects of APF530 on electrocardiographic intervals.Methods: This single-site, double-blind, placebo-controlled, four-period crossover trial randomized healthy men and women to receive varying sequences of APF530 1 g SC, granisetron 50 μg/kg IV, moxifloxacin 400 mg PO, and placebo. Subjects were assessed for 49 hours after each treatment. The primary objective was to evaluate differences between baseline-adjusted, heart rate-corrected QT-interval change using the Fridericia rate correction (dQTcF for APF530 1 g SC and placebo. Electrocardiograms were performed at various times throughout the assessment period. Pharmacokinetics and safety were evaluated.Results: The upper one-sided 95% confidence interval (CI for mean baseline-adjusted dQTcF at each post-dose time point between APF530 and placebo excluded 10 ms, indicating that APF530 1 g SC had no clinically significant effect on QTcF. Maximum observed QTcF change was 4.15 ms (90% CI, 0.94 to 7.36 at Hour 3. No clinically significant changes in other electrocardiogram intervals were observed. APF530 SC pharmacokinetics were as expected, with slow absorption (maximum plasma concentration 35.8 ng/mL, median time to maximum plasma concentration 11.1 hours and slow elimination (mean half-life 18.6 hours

  7. 妇科千金片联合莫西沙星治疗急性盆腔炎60例临床分析%The Clinical Analysis of Fukeqianjin Tablets Combined with Moxifloxacin in Treatment of 60 Patients with Acute Pelvic Inflammatory

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

      目的:探讨妇科千金片联合莫西沙星治疗急性盆腔炎的临床效果。方法:将2009年2月至2011年2月收治的急性盆腔炎患者120例随机分为观察组和对照组各60例,观察组采用妇科千金片联合莫西沙星治疗,对照组采用头孢三嗪联合替硝唑治疗,比较两组临床效果。结果:观察组疗效明显优于对照组(P <0.05),两组不良反应发生率无显著性差异(P >0.05)。结论:妇科千金片联合莫西沙星治疗急性盆腔炎的效果优于头孢三嗪联合替硝唑,且不良反应少,建议临床推广应用。%Objective:To explore the clinical effect of Fukeqianjin tablets combined with moxifloxacin for patients with acute pelvic inflammation. Methods: 120 patients with acute pelvic inflammatory were randomly divided into observation group and control group from Feb 2009 to Feb 2011, each group was 60 cases. The observation group was treated with Fukeqianjin tablets combined with moxifloxacin, while the control group was treated by triazine combined with tinidazole. The clinical effect was compared between the two groups. Results: The clinical effect was significantly better in the observation group than that in the control group, P 0.05. Conclusion: The clinical effect of Fukeqianjin tablets combined with moxifloxacin was better than triazine combined with tinidazole for patients with acute pelvic inflammation and less adverse reaction, so it is worth popularizing in clinic.

  8. Comparative Studv of Moxifloxacin and Piperacillin Sulbactam Sodium in Treatment of Community Acquired Pneumonia%莫西沙星与哌拉西林舒巴坦钠治疗社区获得性肺炎的比较研究

    Institute of Scientific and Technical Information of China (English)

    李芝兰; 郭胜利; 范永红

    2012-01-01

    目的 比较莫西沙星与哌拉西林舒巴坦钠治疗社区获得性肺炎的临床疗效. 方法 2010年3月-2011年3月,98例社区获得性肺炎患者随机分为两组,A组49例采用莫西沙星治疗,B组49例采用哌拉西林舒巴坦钠治疗,比较观察两组的临床疗效以及主要症状、体征恢复情况. 结果 A组的总有效率和细菌清除率明显高于B组(P<0.05).A组咳嗽消失、体温恢复正常、血常规恢复正常和胸部影像明显吸收时间明显短于B组(P<0.05). 结论 与哌拉西林舒巴坦钠比较,莫西沙星对社区获得性肺炎进行治疗,疗效显著,并且患者症状、体征恢复较快,是治疗获得性肺炎的良好选择.%Objective To compare the clinical efficacy of moxifloxacin and piperacillin sulbactam sodium in the treatment of community acquired pneumonia. Methods Ninety- eight patients with community acquired pneumonia hospitalized in the 264 Hospital of Chinese PLA from March 2010 to March 2011 were randomly divided group A and group B (each n=49). Group A was treated with moxifloxacin, while group B was received piperacillin sulbactam sodium. The effective rate, the main clinical symptoms and physical signs were compared between the two groups. Results The total effective rate and bacterial clearance rate in group A were significantly higher than those in group B (P<0.05). The time of cough disappearance, the duration of returning to normal body temperature, returning to normal blood, and obvious absorption of pneumonic foci in group A were significantly shorter than those in group B (P<0.05). Conclusions Compared with piperacillin sulbactam sodium, moxifloxacin has the advantages of good therapeutic effects and rapid recovery of the patients in the treatment of community acquired pneumonia. It is a good choice for community acquired pneumonia.

  9. Pre-Column derivatization Chiral HPLC Method for the separation and quantification of (R,R-2,8-diazobicyclo [4.3.0]nonane content in (S,S-2,8-diazobicyclo[4.3.0]nonane, A Key Intermediate of Moxifloxacin Hydrochloride

    Directory of Open Access Journals (Sweden)

    Bulusu Lakshmi sushma

    2015-12-01

    Full Text Available A simple and robust analytical method was developed for the separation and quantification of (R,R-isomer in (S,S-2,8-diazobicyclo[4.3.0]nonane, a non chromophoric moiety and key intermediate in the synthesis of Moxifloxacin hydrochloride by using pre-column derivatization reagent 4-Chloro-7-nitrobenzofurazan (NBD Chloride. The derivatization was brought to be optimized at room temperature. The separation was achieved on Chiralpak IC 250 mm × 4.6 mm, 5 µm column with isocratic elution mode with a flow rate of 1.0 mL min-1. The mobile phase was a mixture of methanol, ethanol and diethyl amine in the ratio of 1:1:0.1 (v/v/v. The eluents were monitored at 340 nm using a UV detector.

  10. 七氟烷麻醉对新生7d大鼠学习记忆功能及S100β蛋白的影响%Effects of sevoflurane anesthesia on learning and memory function and S100β protein in the 7-day-old rat

    Institute of Scientific and Technical Information of China (English)

    唐冬梅; 高琳; 徐桂萍; 苏涛; 马雪萍

    2015-01-01

    Objective To investigate the effects of sevoflurane anesthesia on learning and memory functions and S100β protein in anaplasis in the 7-day-old rat.Methods 48 SD rats of both sexes aged 7-day-old weighing 1 2-1 6 g were randomly divided into 3 groups (1 6 rats in each group):group A and group B inhaled 3 % sevoflurane in oxygen(1L/min) for 6h and 2h respectively; group C inhaled oxygen(1L/min) only.After inhalation,the effects of sevoflurane anesthesia on learning and memory function were assessed by Morris water-maze test and Y-maze test from 16d to 24d.The rats were killed in the day of 8d and 25d respectively,and the blood were collected for the expression of serum concentration S100β protein by enzyme-linked immunosorbent assay.Results (1) Results of Morris water-maze test:Compared with group C,escape latency prolonged in group A and group B in 17-20d(P < 0.05 or 0.01) ;escape latency prolonged in group A compared with group B in 19-20d(P < 0.01) ;but there were no significant differences in the probe time in original platform quadrant and the frequency of crossing original platform among three groups(P >0.05).(2) Result of Y-maze test:In 22d,the total reaction time of group A and B were longer than group C (P <0.05 or 0.01),and the error number was increased in group A and B compared with group C(P < 0.01) ; while in 23-24d,there were no significant differences between every index of each groups (P > 0.05).(3) Results of the blood serum index:In 8d,serum concentration of S100β protein was significantly increased in group A and B compared with group C (P <0.01),and serum concentration of S100β protein was significantly increased in group A compared with group B (P < 0.05) ; But there were no significant differences in serum concentration of S100β protein of each groups in 25d(P > 0.05).Conclusion Sevoflurane anesthesia in the 7-day-old rat can temporarily decrease the ability of learning and memory functions in the length of inhalation

  11. 模拟失重环境下腹腔感染大鼠肝脏微循环障碍及抗感染疗效%Effects of simulated microgravity and intravenous moxifloxacin on hepatic microcirculation of rat with abdominal infection

    Institute of Scientific and Technical Information of China (English)

    邓忠伟; 鲍时明; 孙宏伟; 姜福全; 徐冰心; 周金莲; 王平; 张宏文; 崔彦

    2015-01-01

    Objective To investigate the effects of simulated microgravity and intravenous moxifloxacin on hepatic microcirculation of rat with abdominal infection.Methods Sixty healthy male adult Wistar rats were divided into 6 groups (n =10) by random number table,including microgravity + shamoperation (MSO),microgravity + abdominal-infection (MAI),microgravity + abdominal-infection + moxifloxacin (MAIM),normal-gravity + sham-operation (NGSO),normal-gravity + abdominal-infection (NGAI) and normal-gravity + abdominal-infection + moxifloxacin (NGAIM).Tail-suspension was used to simulate the weightlessness animal model,and cecal ligation and puncture (CLP) model was used to mimic the abdominal infection.According to the experimental design,the CLP procedure was performed in the rats under simulated weightlessness for 48 hours.The injections of moxifloxacin hydrochloride were given via tail vein to the animals of group MAIM and group GAIM at 0 hour,24 hours and 48 hours post-CLP respectively (30 mg/kg).At 60 hours post-CLP,blood and liver samples were obtained for analysis of serum NO by Nitrate reductase method,ET-1 and iNOS in liver by Western blotting and real-time PCR respectively.The pathological changes of the liver were observed under light microscope.Results The light microscope revealed that the hepatic sinusoids were enlarged with local congestion,and there were focal necrosis in the liver tissue and inflammatory cell infiltration in portal area.The pathological alterations in group MAIM and group NGAIM were alleviated than that of in group MAI and group NGAI.The value of serum NO and expression of ET-1 and iNOS in liver were significantly increased in group MAI than that of in group MSO (P < 0.05),and the value of serum NO and expression of ET-1 and iNOS in liver were down-regulated significantly in group MAIM compared with group MAI (P < 0.05).The serum NO and hepatic ET-1 and iNOS in groups of normal gravity showed a similar tendency compared with that of

  12. Evaluation of an Internet, Stage-Based Physical Activity Intervention.

    Science.gov (United States)

    Hager, Ronald L.; Hardy, Aaron; Aldana, Steven G.; George, James D.

    2002-01-01

    Evaluated the impact of online, stage-based materials on exercise behavior and stage of readiness to change. College faculty participated in stage-based, action-message, or control groups. Occupational and leisure activity, 7-day physical activity, exercise self-efficacy, and stage of readiness to change were assessed at baseline and 6 weeks.…

  13. Observation on curative effect of moxifloxacin in the treatment of initial-treated smear positive pulmonary tuberculosis patients during intensive chemotherapy%莫西沙星在初治肺结核患者化疗中疗效的观察

    Institute of Scientific and Technical Information of China (English)

    漆沄; 苟超伦; 李炜; 苗凤济; 李冬; 周倩

    2016-01-01

    目的:观察含莫西沙星抗结核方案在初治涂阳肺结核患者强化期化疗中的疗效。方法临床选取3年间我院收治的符合纳入及排除标准的涂阳初治肺结核患者93例。随机分为观察组和对照组,观察组在强化期给予HRZE常规抗结核方案基础上加用莫西沙星,对照组给予HRZE常规抗结核方案。对强化期各治疗时间点的痰菌阴转情况和其他临床指标进行分析。结果观察组第4、6、8周痰结核菌阴转率明显高于对照组(P0. 05). The adverse reactions of the two groups were similar (P>0. 05). Conclusion Moxifloxacin-containing anti-TB program can reduce sputum negative conversion time and improve the negative conversion rate and lesions absorption efficiency in the treatment of initial-treated smear positive pulmonary tuberculosis patients during intensive chemotherapy, with the similar adverse reactions as the control group.

  14. Vivax malaria in children: recurrences with standard total dose of primaquine administered in 3 vs. 7 days Malaria vivax en niños: recurrencias con dosis total estándar de primaquina administrada durante 3 frente a 7 días

    Directory of Open Access Journals (Sweden)

    Jaime Carmona-Fonseca

    2010-02-01

    Full Text Available

    Introduction: Worldwide, the efficacy of cloroquine-primaquine for treating acute Plasmodium vivax malarious attacks has not been thoroughly evaluated. In Latin America such studies are scarce, and in Colombia, almost nonexisting.

    Objective: To assess the efficacy of two regimens for administration of primaquine in children aged less than 18 years.

    Methodology: A clinical, controlled, unmasked study was carried out, with randomized administration of two primaquine regimens, namely: 0.50 mg/kg/day for 7 days (0.50-7 vs. 1.17 mg/kg/day for 3 days (1.17-3.

  15. 莫西沙星对老年肾功能不全社区获得性肺炎的治疗效果及安全性评价%Efficiency and safety of moxifloxacin in treatment of elderly community acquired pneumonia with renal dysfunction

    Institute of Scientific and Technical Information of China (English)

    杭晨晨; 王烁

    2014-01-01

    Objective To evaluate the effectiveness and safety of moxifloxacinin treatment of community acquired pneumonia in elderly patients with renal dysfunction , so as to provide safe and effective antibiotics for the treatment of community acquired pneumonia for patients . elderly renal dysfunction .Methods A total of 128 elderly patients with community acquired pneumonia , who enrolled the emergency department of the hospital from Jan 2011 to Dec 2013 , were divided into two groups according to the renal function .The normal group ( n =63 ) and the renal dysfunction group without hemodialysis ( n =65 ) were both treated with moxifloxacin .The clinical efficacies and the adverse reactions were observed .Results There was no statistically difference in the clinical effective rates (81.0% vs.87.7%, P=0.294), the pathogensclearance rate (80.56% vs.88.37%, P=0.335) and the incidence of the adverse reactions (7.93% vs.9.23%, P=0.794) between the two groups.There was no significant difference of moxifloxacin plasma concentration between the two groups at each time point.The plasma concentration was stable in the whole protocol .Conclusion Moxifloxacincan achieve good clinic effect in the treatment of community acquired pneumonia with renal dysfunction in elderly patients with less adverse reaction .%目的:评估莫西沙星在老年肾功能不全社区获得性肺炎( community acquired pneumonia, CAP)中的治疗效果及安全性,为临床治疗老年肾功能不全CAP选择安全、高效的抗菌药物。方法选取2011-01~2013-12在急诊科住院的128例老年CAP患者,根据肾功能状况分为肾功能正常组(n=63)和肾功能不全但未透析组(n=65),均给予莫西沙星治疗,观察临床疗效及不良反应。结果肾功能正常组与肾功能不全组比较,治疗有效率(81.0%vs.87.7%,P=0.294),细菌清除率(80.56%vs.88.37%,P=0.335),不良反应发生率(7.93%vs.9

  16. The clinical effect of moxifloxacin combined cefalexin in the treatment of lower respiratory tract bacterial infections in elderly patients%莫西沙星联合头孢氨苄治疗老年患者下呼吸道细菌感染的临床疗效

    Institute of Scientific and Technical Information of China (English)

    王永红; 冉茂娟; 龙晓莉

    2015-01-01

    OBJECTIVE To investigate the clinical effect and safety of moxifloxacin combined cefalexin in the treatment of lower respiratory tract bacterial infections in elderly patients so as to provide basis for clinical treatment .METHODS Totally 100 cases of elderly patients with lower respiratory tract bacterial infections treated from Mar .2013 to Mar .2014 were collected and were divided into the observation group and the control group with 50 cases in each group according to the random number table method .The observation group was given moxifloxacin combined cefalexin for treatment while the control group was given cefalexin for treatment , the clinical efficacy of two groups were compared .Data were analyzed by the statistical software SPSS 17 .0 .RESULTS Totally 103 strains of pathogens were detected in the elderly patients ,including 64 gram‐negative bacteria ,20 gram‐positive bacteria and 19 fungi ,accounting for 62 .1% ,19 .4% ,and 18 .5% .The effective rate in the observation group was 92 .0% ,significantly higher than 72 .0% in the control group (χ2 = 10 .31 ,P< 0 .05) . There was no significant difference (P<0 .05) between two groups of patients in the recovery of body temperature and white blood cell count as well as the disappearance time of physical signs and clinical symptoms such as cough and pulmonary rales .There were 4 cases of adverse reactions in the observation group ,and 5 cases in the control group ,the difference in occurrence of adverse reactions was not significant between the two groups .CONCLUSION Moxifloxacin combined cefalexin has an obvious clinical treatment effect in elderly patients with lower respiratory tract infections ,and the adverse reactions are few ,it can be used as an important method of treatment for elderly lower respiratory tract infections .%目的:探讨莫西沙星联合头孢氨苄治疗老年患者下呼吸道细菌感染的临床疗效及安全性,为临床治疗提供依据。方法收集2013年3月-2014

  17. Listeria monocytogenes and Escherichia coli septicemia and meningoencephalitis in a 7-day-old llama.

    OpenAIRE

    Frank, N.; Couëtil, L L; Clarke, K. A.

    1998-01-01

    Listeria monocytogenes and Escherichia coli were isolated from blood collected on presentation and tissues samples taken postmortem. Listeria monocytogenes was isolated from cerebrospinal fluid collected antemortem. The importance of passive transfer of immunity, the subtlety of neurologic signs in early meningitis, and considering blood-CSF penetration in antimicrobial selection are discussed.

  18. 29 CFR 2530.200b-7 - Day of service for employees in the maritime industry.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Day of service for employees in the maritime industry. 2530.200b-7 Section 2530.200b-7 Labor Regulations Relating to Labor (Continued) EMPLOYEE BENEFITS SECURITY... industry. (a) General rule. A day of service in the maritime industry which must, as a minimum, be...

  19. Milk-derived proteins and minerals alter serum osteocalcin in prepubertal boys after 7 days

    DEFF Research Database (Denmark)

    Mark, A.B.; Hoppe, Camilla; Michaelsen, K.F.;

    2010-01-01

    growth depending on the content of milk minerals (calcium and phosphorus). This was a randomized, parallel, double-blind study. Eight-year-old boys (n = 57) received 1 of 4 milk drinks: whey protein with low or high content of minerals, or casein protein with low or high content of minerals. The amount...... significantly affected by the treatments (P <.05). There was a significant interaction between milk-derived proteins and minerals with regard to sOC (P = .01). The intake of milk drinks containing whey increased sOC at the low content of minerals, whereas it decreased sOC at the high content of minerals (P <.05......). In contrast, milk drinks containing casein increased sOC both at the low and at the high contents of minerals. In conclusion, whey and casein (corresponding to their content in 1.5 L of milk) differently affect sOC in 8-year-old boys depending on the content of milk minerals, but do not seem to...

  20. A Model Humanitarian Cleft Mission: 312 Cleft Surgeries in 7 Days

    Directory of Open Access Journals (Sweden)

    Ghulam Qadir Fayyaz, MBBS, DSS, MS

    2015-03-01

    Conclusion: A locoregional cleft team can be more effective to care for the patients with cleft in countries where surgical and other expertise can be utilized by proper organization of cleft missions on a national level.

  1. Dark Matter Results from First 98.7-day Data of PandaX-II Experiment

    CERN Document Server

    Tan, Andi; Cui, Xiangyi; Chen, Xun; Chen, Yunhua; Fang, Deqing; Fu, Changbo; Giboni, Karl; Giuliani, Franco; Gong, Haowei; Hu, Shouyang; Huang, Xingtao; Ji, Xiangdong; Ju, Yonglin; Lei, Siao; Li, Shaoli; Li, Xiaomei; Li, Xinglong; Liang, Hao; Lin, Qing; Liu, Huaxuan; Liu, Jianglai; Lorenzon, Wolfgang; Ma, Yugang; Mao, Yajun; Ni, Kaixuan; Ren, Xiangxiang; Schubnell, Michael; Shen, Manbin; Shi, Fang; Wang, Hongwei; Wang, Jiming; Wang, Meng; Wang, Qiuhong; Wang, Siguang; Wang, Xuming; Wang, Zhou; Wu, Shiyong; Xiao, Xiang; Xie, Pengwei; Yan, Binbin; Yang, Yong; Yue, Jianfeng; Zeng, Xionghui; Zhang, Hongguang; Zhang, Hua; Zhang, Huanqiao; Zhang, Tao; Zhao, Li; Zhou, Jing; Zhou, Ning; Zhou, Xiaopeng

    2016-01-01

    We report the WIMP dark matter search results using the first physics-run data of the PandaX-II 500 kg liquid xenon dual-phase time-projection chamber, operating at the China JinPing Underground Laboratory. No dark matter candidate is identified above background. In combination with the data set during the commissioning run, with a total exposure of 3.3$\\times10^4$ kg-day,the most stringent limit to the spin-independent interaction between the ordinary and WIMP dark matter is set for a range of dark matter mass between 3.5 and 1000 GeV/c$^2$. The best upper limit on the scattering cross section is found $2.5\\times 10^{-46}$ cm$^2$ for the WIMP mass 40 GeV/c$^2$ at 90% confidence level.

  2. N-Nitroso compounds: Assessing agreement between food frequency questionnaires and 7-day food records

    Science.gov (United States)

    N-nitroso compounds are recognized as important dietary carcinogens. Accurate assessment of N-nitroso intake is fundamental to advancing research regarding its role with cancer. Previous studies have not used a quantitative database to estimate the intake of these compounds in a US population. To ad...

  3. Ketamine Affects the Neurogenesis of the Hippocampal Dentate Gyrus in 7-Day-Old Rats.

    Science.gov (United States)

    Huang, He; Liu, Cun-Ming; Sun, Jie; Hao, Ting; Xu, Chun-Mei; Wang, Dan; Wu, Yu-Qing

    2016-08-01

    Ketamine has been reported to cause neonatal neurotoxicity via a neuronal apoptosis mechanism; however, no in vivo research has reported whether ketamine could affect postnatal neurogenesis in the hippocampal dentate gyrus (DG). A growing number of experiments suggest that postnatal hippocampal neurogenesis is the foundation of maintaining normal hippocampus function into adulthood. Therefore, this study investigated the effect of ketamine on hippocampal neurogenesis. Male Sprague-Dawley rats were divided into two groups: the control group (equal volume of normal saline), and the ketamine-anesthesia group (40 mg/kg ketamine in four injections at 1 h intervals). The S-phase marker 5-bromodeoxyuridine (BrdU) was administered after ketamine exposure to postnatal day 7 (PND-7) rats, and the neurogenesis in the hippocampal DG was assessed using single- or double-immunofluorescence staining. The expression of GFAP in the hippocampal DG was measured by western blot analysis. Spatial reference memory was tested by Morris water maze at 2 months after PND-7 rats exposed to ketamine treatment. The present results showed that neonatal ketamine exposure significantly inhibited neural stem cell (NSC) proliferation, decreased astrocytic differentiation, and markedly enhanced neuronal differentiation. The disruptive effect of ketamine on the proliferation and differentiation of NSCs lasted at least 1 week and disappeared by 2 weeks after ketamine exposure. Moreover, the migration of newborn neurons in the granule cell layer and the growth of astrocytes in the hippocampal DG were inhibited by ketamine on PND-37 and PND-44. Finally, ketamine caused a deficit in hippocampal-dependent spatial reference memory tasks at 2 months old. Our results suggested that ketamine may interfere with hippocampal neurogenesis and long-term neurocognitive function in PND-7 rats. These findings may provide a new perspective to explain the adult neurocognitive dysfunction induced by neonatal ketamine exposure. PMID:26966008

  4. Sevoflurane exposure in 7-day-old rats affects neurogenesis,neurodegeneration and neurocognitive function

    Institute of Scientific and Technical Information of China (English)

    Fang Fang; Zhanggang Xue; Jing Cang

    2012-01-01

    Objective Sevoflurane is widely used in pediatric anesthesia and former studies showed that it causes neurodegeneration in the developing brain.The present study was carried out to investigate the effects of sevoflurane on neurogenesis,neurodegeneration and behavior.Methods We administered 5-bromodeoxyuridine,an S-phase marker,before,during,and after 4 h of sevoflurane given to rats on postnatal day 7 to assess dentate gyrus progenitor proliferation and Fluoro-Jade staining for degeneration.Spatial reference memory was tested 2 and 6 weeks after anesthesia.Results Sevoflurane decreased progenitor proliferation and increased cell death until at least 4 days after anesthesia.Spatial reference memory was not affected at 2 weeks but was affected at 6 weeks after sevoflurane administration.Conclusion Sevoflurane reduces neurogenesis and increases the death of progenitor cells in developing brain.This might mediate the lateonset neurocognitive outcome after sevoflurane application.

  5. Is a 7-day Helicobater pylori treatment enough for eradication and inactivation of gastric inflammatory activity?

    Institute of Scientific and Technical Information of China (English)

    Carlos Robles-lara; Carlos Robles-Medranda; Manuel Moncayo; Byron Landivar; Johnny Parrales

    2008-01-01

    AIM- To compare the efficacy of a 7-d vs 10-d triple therapy regarding H pylori eradication, endoscopic findings and histological gastric inflammatory inactivation in the Ecuadorian population. METHODS: 136 patients with dyspepsia and H pylori infection were randomized in 2 groups (68 per group): group 1, 7-d therapy; group 2, t0-d therapy. Both groups received the same medication and daily dosage: omeprazole 20 mg bid, clarithromycin 500 mg bid and amoxicillin I g bid. Endoscopy was performed for histological assessment and H pylori infection status before and 8 wk after treatment. RESULTS: H pylori was eradicated in 68% of group 1 vs 83. 8% of group 2 for the intention-to-treat analysis (ITT) (P = 0. 03; OR = 2. 48; 95% CI, 1. 1-5. 8), and 68% in group 1 vs 88% in group 2 for the per-protocol analysis (PP) (P = 0. 008; OR = 3. 66; 95% CI, 1. 4-10). Endoscopic gastric mucosa normalization was observed in 56. 9% in group 1 vs 61. 2% in group 2 for ITT, with similar results for the PP, the difference being statistically not significant. The rate of inflammatory inactivation was 69% in group 1 vs 88. 7% in group 2 for ITT (P = 0. 007;OR = 3. 00; 95% CI, 1. 2-7. 5), and 69% in group 1 vs96% in group 2 for PP (P = 0. 0002; OR = 7. 25; 95% CI, 2-26). CONCLUSION: In this Ecuadorian population, the 10-d therapy was more effective than the 7-d therapy for H pylori eradication as well as for gastric mucosa inflammatory inactivation.

  6. Partial restoration of dietary fat induced metabolic adaptations to training by 7 days of carbohydrate diet

    DEFF Research Database (Denmark)

    Helge, Jørn Wulff; Watt, Peter W; Richter, Erik A;

    2002-01-01

    We tested the hypothesis that a shift to carbohydrate diet after prolonged adaptation to fat diet would lead to decreased glucose uptake and impaired muscle glycogen breakdown during exercise compared with ingestion of a carbohydrate diet all along. We studied 13 untrained men; 7 consumed a high......-fat (Fat-CHO; 62% fat, 21% carbohydrate) and 6 a high-carbohydrate diet (CHO; 20% fat, 65% carbohydrate) for 7 wk, and thereafter both groups consumed the carbohydrate diet for an eighth week. Training was performed throughout. After 8 wk, during 60 min of exercise (71 +/- 1% pretraining maximal oxygen...... +/- 59 vs. 688 +/- 43 mmol/kg dry wt) in Fat-CHO than in CHO. In conclusion, shift to carbohydrate diet after prolonged adaptation to fat diet and training causes increased resting muscle glycogen levels but impaired leg glucose uptake and similar muscle glycogen breakdown, despite higher resting levels...

  7. Eficácia do regime terapêutico empregando a associação de pantoprazol, claritromicina e amoxicilina, durante uma semana, na erradicação do Helicobacter pylori em pacientes com úlcera péptica Efficacy of the dosing regimen of pantoprazole 40 mg, amoxicillin 1000 mg and clarithromycin 500 mg, twice daily for 7 days, in the eradication of Helicobacter pylori in patients with peptic ulcer

    Directory of Open Access Journals (Sweden)

    Luiz Gonzaga Vaz Coelho

    2004-03-01

    : Seventy-one patients (36 females, 35 males, average age 41.9 years from three Brazilian university centers (located in the cities of Belo Horizonte and Porto Alegre, with peptic ulcers confirmed by endoscopy, and infections by H. pylory proven by at least two diagnostic testings were admitted in the trial. An association of pantoprazole 40 mg, clarithromycin 500 mg and amoxicillin 1.0 g was administered to patients twice daily for 7 days. RESULTS: By the end of treatment all patients were examined for digestive symptoms, presence of adverse events, and treatment adherence. Sixty days after the end of the treatment a new endoscopy with biopsies and respiratory function testing with 13C-urea breath test was performed in order to determine the eradication rates of that microorganism. Patients showing negative results at least in the 13C-urea breath test and in one other test (urease or histology were considered H. pylory-negative. By the end of the trial, 60/69 (87%, CI 95% = 78.9-94.8 patients had the H. pylory eradicated in the per protocol analysis and 60/71 (84.5%, CI 95% = 76-92.9 in the intention-to-treat analysis. One patient was withdrawn from the trial due to a diarrhea. Twelve (16.9% patients showed adverse symptoms that were deemed as mild symptoms. CONCLUSION: Our conclusion is that the association of pantoprazole, amoxicillin and clarithromycin administered during 7 days is an effective and well-tolerated alternative as regards the eradication of H. pylory in patients with peptic ulcer in Brazil.

  8. Improved extraction of fluoroquinolones with recyclable ionic-liquid-based aqueous biphasic systems†

    Science.gov (United States)

    Almeida, Hugo F. D.; Freire, Mara G.; Marrucho, Isabel M.

    2016-01-01

    In the past few years, the improvement of advanced analytical tools allowed to confirm the presence of trace amounts of metabolized and unchanged active pharmaceutical ingredients (APIs) in wastewater treatment plants (WWTPs) as well as in freshwater surfaces. It is known that the continuous contact with APIs, even at very low concentrations (ng L−1–μg L−1), leads to serious human health problems. In this context, this work shows the feasibility of using ionic-liquid-based aqueous biphasic systems (IL-based ABS) in the extraction of quinolones present in aqueous media. In particular, ABS composed of imidazolium- and phosphonium-based ILs and aluminium-based salts (already used in water treatment plants) were evaluated in one-step extractions of six fluoroquinolones (FQs), namely ciprofloxacin, enrofloxacin, moxifloxacin, norfloxacin, ofloxacin and sarafloxacin, and extraction efficiencies up to 98% were obtained. Despite the large interest devoted to IL-based ABS as extractive systems of outstanding performance, their recyclability/reusability has seldomly been studied. An efficient extraction/cleaning process of the IL-rich phase is here proposed by FQs induced precipitation. The recycling of the IL and its further reuse without losses in the ABS extractive performance for FQs were established, as confirmed by the four consecutive removal/extraction cycles evaluated. This novel recycling strategy supports IL-based ABS as sustainable and cost-efficient extraction platforms.

  9. Are Broad-Spectrum Fluoroquinolones More Likely To Cause Clostridium difficile-Associated Disease?

    OpenAIRE

    Dhalla, Irfan A.; Muhammad M Mamdani; Simor, Andrew E; Kopp, Alex; Rochon, Paula A; Juurlink, David N.

    2006-01-01

    Limited evidence suggests that broad-spectrum fluoroquinolones such as gatifloxacin and moxifloxacin are more likely to cause Clostridium difficile-associated disease than levofloxacin. In a population-based case-control study of outpatients prescribed fluoroquinolones, we found no increased risk of C. difficile-associated disease requiring hospitalization among patients prescribed gatifloxacin or moxifloxacin compared to levofloxacin.

  10. DEVELOPMENT OF COMPOSITION OF INFUSION MEDICATION ON BASIS OF MOXIFLOXACIN

    OpenAIRE

    Almakaeva L.G.; Begunova N.V.; Naumenok L.G.; Dolya V.G.; Almakaev М.S.

    2015-01-01

    Application of fluoroquinolones covers by experience of treatment more than 800 million patients, and presently they are one of basic classes in the antimicrobial arsenal of practical medicine. Such achievements became possible after the clear understanding of intercommunication of structure and activity of molecules of this class of antibiotics. This knowledge became the basis for the synthesis of new derivatives with a wide range, powerful activity and improved pharm...

  11. Base

    DEFF Research Database (Denmark)

    Hjulmand, Lise-Lotte; Johansson, Christer

    2004-01-01

    BASE - Engelsk basisgrammatik er resultatet af Lise-Lotte Hjulmands grundige bearbejdning og omfattende revidering af Christer Johanssons Engelska basgrammatik. Grammatikken adskiller sig fra det svenske forlæg på en lang række punkter. Den er bl.a. tilpasset til et dansk publikum og det danske...

  12. Influence of moxifloxacin on lipoteichoic acid induced apoptosis and expression of inflammatory cytokines in human alveolar macrophage%莫西沙星对脂磷壁酸诱导的人肺泡巨噬细胞凋亡及炎症因子释放的影响

    Institute of Scientific and Technical Information of China (English)

    罗进梅; 吴本权; 刘慧; 李洪涛; 黄静; 朱家馨; 张天托

    2012-01-01

    Objective To investigate the lipoteichoic acid(LTA) induced apoptosis and the expression of inflammatory cytokines in human alveolar macrophage (AM) and the anti-apoptotic and anti-inflamatory effect of moxifloxacin (MXF).Methods Obtained human AM from bronchoalveolar lavage and used MTT assay to observe the effects of LTA and MXF on cell activity,optical microscope to investigate the change of the cell morphology,flow cytometry to assess cell apoptosis,RT-PCR to detect the mRNA levels of TLR2,IL-1 β,IL-8 and TNF-α,ELISA for the production of IL-8 to exam RT-PCR.Results LTA showed cytotoxicity on AM in a dose-dependent manner ( P<0.05 ) ; MXF inhibited the effect of LTA without cytotoxicicy ( P<0.05 ).LTA promoted apoptosis ( P<0.05 ) and the mRNA expressions of TRL2,IL-1 β,IL-8 and TNF-α significantly in AM (P<0.05),the peaks and peak time ofthe above factors were (3.56±0.03) at 12 h,(46.63±7.06) at 6 h,(28.07±1.24) at 12 h and (2.34 ±0.50) at 3 h respectively and increased the release of IL-8 protein level at 24 h (P<0.05).MXF inhibited the cell apoptosis and the above mRNA expression at 12h ( P<0.05 ),and inhibited the IL-8 protein level at 24 h( P<0.05 ).Conclusion LTA showed cytotoxicity on AM,induced AM apoptosis and increased the expression of TLR2,IL-I β,IL-8 and TNF-α of AM ; MXF could protect AM through inhibiting of the above effects and may play a key role beside bactericidal effect in gram-positive bacteria pneumonia.%目的 探讨脂磷壁酸(LTA)对人肺泡巨噬细胞(AM)凋亡及炎症因子释放的影响和莫西沙星(MXF)对其反应的抑制作用.方法 收集、提纯及体外培养人AM,LTA刺激4h后,加或不加MXF与其共孵育,于各实验终点用MTT法计算细胞相对活力,光学显微镜观察细胞形态,流式细胞术检测细胞凋亡率,RT-PCR法检测TLR2、IL-1β、IL-8及TNF-α的mRNA水平,ELISA检测IL-8蛋白水平,验证RT-PCR.结果 LTA对AM有细胞毒性,并呈浓度递增关系(P<0

  13. [Dimensional changes of silver and gallium-based alloy].

    Science.gov (United States)

    Ballester, R Y; Markarian, R A; Loguercio, A D

    2001-01-01

    Gallium-based dental alloys were created with the aim of solving the problem of toxicity of mercury. The material shows mechanical properties similar to those of dental amalgam, but researches point out two unfavorable characteristics: great corrosion and excessive post-setting expansion, and the latter is capable of cracking dental structures. The aim of this study was to evaluate, during 7 days, the in vitro dimensional alteration of a gallium dental alloy (Galloy, SDI, Australia), in comparison with a dental amalgam containing zinc (F400, SDI, Australia), as a function of the contact with saline solution (0.9% NaCl) during the setting period. The storage experimental conditions were: storage in dry environment, immersion in saline solution and contamination during condensation. Additionally, the effects of contamination during the trituration of dental amalgam and the effects of protecting the surface of the gallium alloy with a fluid resin were studied. Specimens were stored at 37 degrees C +/- 1 degree C, and measuring was carried out, sequentially, every 24 h during 7 days. When the gallium alloy was either contaminated or immersed, an expansion significantly greater than that observed in the other experimental conditions was noticed after 7 days. The application of a fluid resin to protect the surface of the cylinders was able to avoid the increase in expansion caused by superficial moisture. The amalgam alloy did not show significant dimensional alterations, except when it was contaminated during trituration.

  14. Anthropometric surrogates for screening of low birth weight newborns: a community-based study.

    Science.gov (United States)

    Rustagi, Neeti; Prasuna, J G; Taneja, D K

    2012-03-01

    In developing countries, where about 75% of births occur at home or in the community, logistic problems prevent the weighing of every newborn child. This study compares various anthropometric surrogates for identification of low birth weight neonates. A longitudinal community based study was done in an urban resettlement colony and 283 singleton neonates within 7 days of birth were examined for the anthropometric measurements such as head, chest, mid upper arm circumference and foot length as a screening tool for low birth weight. Chest circumference measured within 7 days of birth appeared to be the most appropriate surrogate of low birth weight with highest sensitivity (75.4%), specificity (78.4%), and positive predictive value (48.9%) as compared with other anthropometric parameters. Low birth weight neonates in absence of weighing scales can be early identified by using simple anthropometric measurements for enhanced home-based care and timely referral.

  15. Dentinal shear bond strength, microleakage, and contraction gap of visible light-polymerized liners/bases.

    Science.gov (United States)

    Cooley, R L; Barkmeier, W W

    1991-06-01

    The bond strength and microleakage patterns of three light-curing glass-ionomer cement liners/bases (Vitrebond, XR Ionomer, and Zionomer) were evaluated and compared to a fluoride-releasing resin (TimeLine) designed for the same use. Bond strength tests were performed at 24 hours and 7 days. At 24 hours Vitrebond, Time-Line, and Zionomer had statistically significantly greater bond strengths than XR Ionomer. At 7 days, Vitrebond had a statistically significantly stronger bond than the others. Microleakage was evaluated after 24 hours of thermocycling. Vitrebond and XR Ionomer had statistically significantly less leakage than the others, while TimeLine had significantly more leakage than the others. Polymerization contraction gaps between the liners/bases and dentin were examined with scanning electron microscopy. Contraction gaps were approximately 10 microns with Vitrebond and XR Ionomer and 5 microns with TimeLine. A contraction gap generally was not observed with Zionomer.

  16. Intraperitoneal inflammatory response to water- and oil-based iodinated contrast material

    International Nuclear Information System (INIS)

    This study was undertaken to evaluate the early (24 hours) and delayed (7 and 30 days) inflammatory response of the peritoneal surface of guinea pigs to the intraperitoneal administration of water- and oil-based iodinated contrast material. The control group (Ringer's lactate) revealed no inflammatory reaction. Conray 60 (iothalamate meglumine) produced minimal reaction at 24 hours and no reaction at 7 or 30 days. Ioxitol (nonionic) yielded minimal inflammation at 24 hours and 30 days, with significant inflammation present only at 7 days. The greatest inflammation for a water soluble agent occurred with Hypaque 50 (diatrizoate sodium), with maximum inflammation identified at 7 days. The animals receiving Ethiodol (oil based) demonstrated minimal inflammation at 24 hours, with progressively increasing reaction at 7 and 30 days. The 30-day group showed a striking inflammatory response with granulamatous features and fibrosis

  17. Diet-related greenhouse gas emissions assessed by a food frequency questionnaire and validated using 7-day weighed food records

    OpenAIRE

    Sjörs, Camilla; Raposo, Sara E; Sjölander, Arvid; Bälter, Olle; Hedenus, Fredrik; Bälter, Katarina

    2016-01-01

    Background: The current food system generates about 25 % of total greenhouse gas emissions (GHGE), including deforestation, and thereby substantially contributes to the warming of the earth’s surface. To understand the association between food and nutrient intake and GHGE, we therefore need valid methods to assess diet-related GHGE in observational studies. Methods: Life cycle assessment (LCA) studies assess the environmental impact of different food items. We linked LCA data expressed as kg ...

  18. Decrease of contractile properties and transversal stiffness of single fibers in human soleus after 7-day “dry” immersion

    Science.gov (United States)

    Ogneva, I. V.; Ponomareva, E. V.; Kartashkina, N. L.; Altaeva, E. G.; Fokina, N. M.; Kurushin, V. A.; Kozlovskaya, I. B.; Shenkman, B. S.

    2011-05-01

    The simulation model of "dry" immersion was used to evaluate the effects of plantar mechanical stimulation (PMS) and high frequency electromyostimulation (EMS) on the mechanical properties of human soleus fibers under the conditions of gravitational unloading. We examined contractile properties of single fibers by means of tensometry, transversal stiffness of sarcolemma and different areas of the contractile apparatus by means of atomic force microscopy. It was shown that there is a reduction of transversal stiffness in single muscle fibers under hypogravitational conditions. Application of different countermeasures could compensate this effect. Meanwhile pneumostimulation and electro stimulation act in quite different way. Therefore, pneumostimulation seems to be more effective. The data obtained can be considered as the evidence of the fact that such countermeasures as PMS and electromyostimulation influence on muscle fibers in quite different ways and PMS efficiency is likely to be higher. On the basis of our experimental data on transverse stiffness of mechanotransductional nodes and the contractile apparatus, we can assume that support stimulation allows prevention of destructive processes in muscle fibers. Electrostimulation seems to stimulate contractile activity only without suppression of impairment of the fiber mechanical properties.

  19. Effects of 7 days of exercise training on insulin sensitivity and responsiveness in type 2 diabetes mellitus

    DEFF Research Database (Denmark)

    Kirwan, John P; Solomon, Thomas; Wojta, Daniel M;

    2009-01-01

    The objectives of this study were to determine whether 1) the improvement in insulin action induced by short-term exercise training in patients with type 2 diabetes is due to an improvement in insulin sensitivity, an improvement in insulin responsiveness, or a combination of improved insulin sens...

  20. Differential effects of erythropoietin on neural and cognitive measures of executive function 3 and 7 days post-administration.

    Science.gov (United States)

    Miskowiak, Kamilla; Inkster, Becky; O'Sullivan, Ursula; Selvaraj, Sudhakar; Goodwin, Guy M; Harmer, Catherine J

    2008-01-01

    Erythropoietin (Epo) has neuroprotective and neurotrophic effects and improves cognitive function in animal models of neurodegenerative and neuropsychiatric illness. In humans, weekly Epo administration over 3 months improves cognitive function in schizophrenia. The neural underpinnings and time-course of this effect of Epo are currently unknown. It is also unclear whether the cognitive improvement reflects direct neurobiological actions or is secondary to hematological effects. We therefore assessed the actions of single administration of Epo (40,000 IU) vs. saline to healthy volunteers on cognitive and neural measures of executive function using a verbal fluency task and N-back working memory (WM) paradigm during functional magnetic resonance imaging (fMRI) on day 3 and 7 after administration in two separate cohorts of subjects. Epo modulated neuronal response in a fronto-parietal network during WM performance at both time points; on day 3 after administration, activation was increased in left-hemisphere frontal and cingulate cortex and reduced in the right parietal cortex; in contrast, neural response was enhanced in a right-lateralized fronto-parietal network and reduced in left-side regions 1 week post-administration. In addition, Epo-treated volunteers displayed improved verbal fluency performance 1 week post-administration. These effects occurred in the absence of changes in hematological measures suggesting that they reflect direct neurobiological actions of Epo. The findings are consistent with enduring effects of Epo on neurotrophic signaling and induction of neurochemical changes over time in neural networks typically affected in neuropsychiatric illness. The present study supports the notion that Epo may have clinical applications in the treatment of psychiatric disorder characterized by cognitive dysfunction.

  1. Eradication of H pylori infection in a rural population:One-day quadruple therapy versus 7-day triple therapy

    Institute of Scientific and Technical Information of China (English)

    Lian Zhang; Wei-Cheng You; Lin Shen; Jun-Ling Ma; Kai-Feng Pan; Wei-Dong Liu; Jie Li; Shu-Dong Xiao; San-Ren Lin; Meinhard Classen

    2006-01-01

    AIM: To compare the one-day quadruple therapy with a standard 7-d triple therapy for H pylori eradication in a rural population of China.METHODS: A total of 396 patients with 13C-urea breath test positive for H pylori were assigned into two groups:239 patients received one-day quadruple therapy (amoxicillin 2000 mg qid; metronidazole 500 mg qid; bismuth citrate 900 mg qid and lansoprazole 60 mg once daily)and 157 patients received 7-d standard triple therapy (amoxicillin 1000 mg bid; clarithromycin 500 mg bid and lansoprazole 30 mg bid). All the patients underwent a 13C-UBT to assess the eradication of H pylori infection six weeks after treatment.RESULTS: Two hundred and twenty-nine patients completed the one-day therapy (95.8%) and 148patients completed the 7-d therapy (94.2%). The oneday therapy eradicated H pylori infection in 64 patients (27.95%). In contrast, 103 patients (69.59%) were H pylori negative after the 7-d therapy (P < 0.01).CONCLUSION: This pilot study suggests there is no beneficial effect of the one-day therapy in treatment of H pylori infection compared with the 7-d standard therapy.

  2. Survey of the vestibulum, and behavior of xenopus laevis larvae developed during a 7-days space flight

    Science.gov (United States)

    Briegleb, W.; Neubert, J.; Schatz, A.; Klein, T.; Kruse, B.

    Aquatic animals have almost no body weight related proprioception for spatial orientation. Xenopus larvae, like fish, maintain their attitude in water by continuous correction with their fin(s). For these reasons a special performance of the equilibrium system compared to terrestrial animals is necessary. Evidently fish therefore have more compact (dense) otoliths; Xenopus larvae have less dense otolith (membranes) similar to land vertebrates; but their sacculus-otoliths are vertically positioned, which also may lead to a higher g-sensitivity. For plausibility reasons gravity should influence the embryonic development of gravity receptors. Yet, evaluations of photographs taken from the surface of cut deep-frozen objects by incident light show no aberration of the shape of the whole vestibulum and of the shape, density, size and position of the otolith membrane in larvae developed under near-zero g (NEXPA-BW-STATEX in D1-Mission). The further evaluation of the ``weightless-larvae'' revealed a probably not yet described statolith-like formation in the dorsal wall of the vestibulum. In the weightless larvae this formation outnumbers, also qualitatively, strongly the 1-g controls. An extra result is the lack of striking effects of cosmic radiation on the embryonic development of the flown Xenopus eggs. The swimming behavior of the larvae which was observed about one hour after landing of the Space Shuttle showed a typical anomaly (loop swimming), which is known from larvae developed on the clinostat or from fish flown aboard Apollo capsules.

  3. An evaluation of the sensitivity of freshwater mussels in 7-day effluent toxicity tests compared to commonly tested species

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The federally endangered Tar River spinymussel (Elliptio steinstansana) is endemic to the Tar River and Neuse River systems in North Carolina. The causes of...

  4. Planktonic and biofilm communities from 7-day-old chicken cecal microflora cultures: Characterization and resistance to Salmonella colonization

    Science.gov (United States)

    Over the last few years, both scientific organizations and regulatory agencies have focused on the use of antimicrobial agents in food animals and the related risk of developing antibiotic resistance. Despite increased information relating to the importance of bacterial biofilms and their potential...

  5. Interaction of silicon-based quantum dots with gibel carp liver: oxidative and structural modifications

    Science.gov (United States)

    Stanca, Loredana; Petrache, Sorina Nicoleta; Serban, Andreea Iren; Staicu, Andrea Cristina; Sima, Cornelia; Munteanu, Maria Cristina; Zărnescu, Otilia; Dinu, Diana; Dinischiotu, Anca

    2013-05-01

    oxidative stress induced 7 days after silicon-based QDs exposure in an efficient manner.

  6. Levofloxacin-based first-line therapy versus standard first-line therapy for Helicobacter pylori eradication: meta-analysis of randomized controlled trials.

    Directory of Open Access Journals (Sweden)

    Musthafa Chalikandy Peedikayil

    Full Text Available BACKGROUND: First-line levofloxacin-based treatments eradicate Helicobacter pylori with varying success. We examined the efficacy and safety of first-line levofloxacin-based treatment in comparison to standard first-line therapy for H pylori eradication. MATERIALS AND METHODS: We searched literature databases from Medline, EMBASE, and the Cochrane Register of Randomized Controlled Trials through March 2013 for randomized controlled trials comparing first-line levofloxacin and standard therapy. We included randomized controlled trials conducted only on naïve H pylori infected patients in adults. A systematic review was conducted. Meta-analysis was performed with Review Manager 5.2. Treatment effect was determined by relative risk with a random or fixed model by the Mantel-Haenszel method. RESULTS: Seven trials were identified with 888 patients receiving 7 days of first-line levofloxacin and 894 treated with standard therapy (Amoxicillin, Clarithromycin and proton pump inhibitor for 7 days. The overall crude eradication rate in the Levofloxacin group was 79.05% versus 81.4% in the standard group (risk ratio 0.97; 95% CI; 0.93, 1.02. The overall dropout was 46 (5.2% in the levofloxacin group and 52 (5.8% for standard therapy. The dizziness was more common among group who took Levofloxacin based treatment and taste disturbance was more common among group who took standard therapy. Meta-analysis of overall adverse events were similar between the two groups with a relative risk of 1.06 (95% CI 0.72, 1.57. CONCLUSION: Helicobacter pylori eradication with 7 days of Levofloxacin-based first line therapy was safe and equal compared to 7 days of standard first-line therapy.

  7. EXPERIMENTAL EVALUATION OF LOW CALCIUM FLY ASH BASED GEOPOLYMER CONCRETE

    Directory of Open Access Journals (Sweden)

    NIRAGI DAVE

    2012-12-01

    Full Text Available There are many ways to reduce environmental pollution that causes by production of Portland cement and cause by the increasing of waste material. Geopolymer concrete results from the reaction of a source material that is rich in silica and alumina with alkaline liquid. In this experiment low calcium (ASTM Class F fly ash based Geopolymer is used as the binder, in the replacement of portland cement to produce Geopolymer concrete. The alkaline liquid that been used in geopolymerisation is the combination of sodium hydroxide (NaOH and sodium metasilicate (Na2Sio2. Fly ash has been collected from Badarpur NTPC plant, New Delhi. In the present study, three (3 series of geopolymer concrete specimens composing molar of sodium hydroxide (NaOH which is 8M was adopted. There are 9 cube specimens at size 150mm x 150mm x 150mm were prepared. The test specimenswere cured at 60 degree in an oven and at ambient condition. The curing time varied from 24 hour to 168 hours (7 days. The result shows that the strength of geopolymer concrete after 24 hour is not superior and after 7 day the compressive strength of Geopolymer concrete is moderate. Therefore further curing period and temperatureshould be improved.

  8. A pilot study on mindfulness based stress reduction for smokers

    Directory of Open Access Journals (Sweden)

    Baker Timothy B

    2007-01-01

    Full Text Available Abstract Background Mindfulness means paying attention in the present moment, non-judgmentally, without commentary or decision-making. We report results of a pilot study designed to test the feasibility of using Mindfulness Based Stress Reduction (MBSR (with minor modifications as a smoking intervention. Methods MBSR instructors provided instructions in mindfulness in eight weekly group sessions. Subjects attempted smoking cessation during week seven without pharmacotherapy. Smoking abstinence was tested six weeks after the smoking quit day with carbon monoxide breath test and 7-day smoking calendars. Questionnaires were administered to evaluate changes in stress and affective distress. Results 18 subjects enrolled in the intervention with an average smoking history of 19.9 cigarettes per day for 26.4 years. At the 6-week post-quit visit, 10 of 18 subjects (56% achieved biologically confirmed 7-day point-prevalent smoking abstinence. Compliance with meditation was positively associated with smoking abstinence and decreases in stress and affective distress. Discussions and conclusion The results of this study suggest that mindfulness training may show promise for smoking cessation and warrants additional study in a larger comparative trial.

  9. Effect of early progressive resistance training compared with home-based exercise after total hip replacement

    DEFF Research Database (Denmark)

    Mikkelsen, Lone Ramer; Mechlenburg, Inger; Søballe, Kjeld;

    Introduction Muscle strength and physical function deficits persist after total hip replacement (THR). Training effect evidence after THR is lacking. This study investigates the effect of supervised progressive resistance training in early post-THR rehabilitation on muscle strength and functional...... performance. Material and Method 73 THR patients with preoperative self-assessed disability (HOOS ADL score≤67) were randomly assigned to a control group (CG, home-based exercise 7 days/week) or an intervention group (IG, home-based exercise 5 days/week and resistance training of hip and thigh muscles 2 days...... in CG (1.58 [0.8;2.4] sec) (p=0.05). No significant differences were found in stair test; yet, borderline significance (p=0.06-0.09) favoured IG in STS and isometric strength. Conclusion 7 days/week of home-based exercise was just as effective as 5 days/week of home-based exercise plus 2 days/week of...

  10. Vegetarianism, low meat consumption and the risk of colorectal cancer in a population based cohort study.

    Science.gov (United States)

    Gilsing, Anne M J; Schouten, Leo J; Goldbohm, R Alexandra; Dagnelie, Pieter C; van den Brandt, Piet A; Weijenberg, Matty P

    2015-01-01

    To study how a vegetarian or low meat diet influences the risk of colorectal cancer compared to a high meat diet, and to assess the explanatory role of factors associated with these diets. In the Netherlands Cohort Study - Meat Investigation Cohort (NLCS-MIC) (cohort of 10,210 individuals including 1040 self-defined vegetarians), subjects completed a baseline questionnaire in 1986, based on which they were classified into vegetarians (n = 635), pescetarians (n = 360), 1 day/week- (n = 1259), 2-5 day/week- (n = 2703), and 6-7 day/week meat consumers (n = 5253). After 20.3 years of follow-up, 437 colorectal cancer cases (307 colon, 92 rectal) were available. A non-significantly decreased risk of CRC for vegetarians, pescetarians, and 1 day/week compared to 6-7 day/week meat consumers was observed (age/sex adjusted Hazard Ratios (HR): 0.73(0.47-1.13), 0.80(0.47-1.39), and 0.72(0.52-1.00), respectively). Most of the differences in HR between these groups could be explained by intake of dietary fiber and soy products. Other (non-)dietary factors characteristic for a vegetarian or low meat diet had negligible individual effects, but attenuated the HRs towards the null when combined. Vegetarians, pescetarians, and 1 day/week meat eaters showed a non-significantly decreased risk of colorectal cancer compared to 6-7 day/week meat consumers, mainly due to differences in dietary pattern other than meat intake. PMID:26316135

  11. Effects of neem-based insecticides on beet armyworm (Lepidoptera: Noctuidae)

    Institute of Scientific and Technical Information of China (English)

    SHOILM.GREENBERG; ALLANT.SHOWLER; TONG-XIANLIU

    2005-01-01

    Three commercial neem [Azadirachta indica A. Juss (Meliaceae)]-based insecticides, Agroneem, Ecozin, and Neemix, and a non-commercial neem leaf powder,were evaluated for oviposition deterrence, antifeedant effect on larvae, and toxicity to eggs and larvae of the beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae),on cotton leaves in the laboratory. Oviposition deterrence in no-choice, and two- and fivechoice assays, was observed for the neem-based insecticide treatments when compared with a non-treated control. Neem-based insecticides also deterred feeding by beet armyworm larvae. Direct contact with neem-based insecticides decreased the survival of beet armyworm eggs. Survival of beet armyworm larvae fed for 7 days on leaves treated with neembased insecticides was reduced to 27, 33, 60, and 61% for neem leaf powder, Ecozin,Agroneem, and Neemix, respectively. Possibilities for adoption of neem-based insecticides in commercial cotton for beet armyworm control are discussed.

  12. Algal-based immobilization process to treat the effluent from a secondary wastewater treatment plant (WWTP)

    Energy Technology Data Exchange (ETDEWEB)

    He Shengbing, E-mail: heshengbing@sjtu.edu.cn [School of Environmental Science and Engineering, Shanghai Jiaotong University, 800 DongChuan Road, Shanghai 200240 (China); Xue Gang [School of Environmental Science and Engineering, Donghua University, Shanghai 200051 (China)

    2010-06-15

    Algal-based immobilization process was applied to treat the effluent from a secondary wastewater treatment plant. Batch test proved that algae could attach onto fiber-bundle carrier in 7 days, and then the algal-based immobilization reactor could reduce TN (total nitrogen) and TP (total phosphorus) significantly within 48 h. Based on the above investigations, the hydraulic retention time (HRT) of the algal-based immobilization reactor in continuous operation mode was determined to be 2 days. During the 91 days of experiment on the treating secondary effluent of Guang-Rao wastewater treatment plant, it was found that the fiber-bundle carrier could collect the heterobacteria and nitrifying bacteria gradually, and thus improved the COD removal efficiency and nitrification performance step by step. Results of the continuous operation indicated that the final effluent could meet the Chinese National First A-level Sewage Discharge Standard when the algal-based immobilization reactor reached steady state.

  13. ALKALI-ACTIVATED CEMENT MORTARS CONTAINING RECYCLED CLAY-BASED CONSTRUCTION AND DEMOLITION WASTE

    Directory of Open Access Journals (Sweden)

    F. Puertas

    2015-09-01

    Full Text Available The use of clay-based waste as an aggregate for concrete production is an amply studied procedure. Nonetheless, research on the use of this recycled aggregate to prepare alkaline cement mortars and concretes has yet to be forthcoming. The present study aimed to determine: the behaviour of this waste as a pozzolan in OPC systems, the mechanical strength in OPC, alkali-activated slag (AAS and fly ash (AAFA mortars and the effect of partial replacement of the slag and ash themselves with ground fractions of the waste. The pozzolanic behaviour of clay-based waste was confirmed. Replacing up to 20 % of siliceous aggregate with waste aggregate in OPC mortars induced a decline in 7 day strength (around 23 wt. %. The behaviour of waste aggregate in AAMs mortars, in turn, was observed to depend on the nature of the aluminosilicate and the replacement ratio used. When 20 % of siliceous aggregate was replaced by waste aggregate in AAS mortars, the 7 day strength values remained the same (40 MPa. In AAFA mortars, waste was found to effectively replace both the fly ash and the aggregate. The highest strength for AAFA mortars was observed when they were prepared with both a 50 % replacement ratio for the ash and a 20 % ratio for the aggregate.

  14. Juvenile nasopharyngeal angiofibroma with skull base invasion : intratumoral direct puncture embolization

    International Nuclear Information System (INIS)

    The purpose of this study is to demonstrate the utility and efficacy of percutaneous direct glue embolization for juvenile nasopharyngeal angiofibromas with skull base invasion. In nine cases of juvenile nasopharyngeal angiofibromas with invasion of the skull base, embolization under general anethesia was performed. Using an 18G spinal needle, direct puncture were made via the transnasal or mandibular sciatic notch. A glue-lipiodol mixture (1:1 -1:3) was injected slowly for 15 to 30 seconds under fluoroscopic control; the number of post-embolization angiography and the distribution of embolic materials was assessed on CT within 1-3 days. The mass was surgically removed 3 to 7 days after embolization. Direct glue embolization of juvenile angiofibroma with skull base invasion appears to be a simple and safe procedure. The technique could be used for other hypervascular lesions in the base of the skull or parapharyngeal space. (author). 19 refs., 1 tab., 2 figs

  15. Moxifloxacin Compared With Ciprofloxacin/Amoxicillin in Treating Fever and Neutropenia in Patients With Cancer

    Science.gov (United States)

    2012-09-20

    Chronic Myeloproliferative Disorders; Fever, Sweats, and Hot Flashes; Infection; Leukemia; Lymphoma; Multiple Myeloma and Plasma Cell Neoplasm; Myelodysplastic Syndromes; Myelodysplastic/Myeloproliferative Neoplasms; Neutropenia; Precancerous Condition; Unspecified Adult Solid Tumor, Protocol Specific

  16. 莫西沙星合成路线图解%Graphical Synthetic Routes of Moxifloxacin

    Institute of Scientific and Technical Information of China (English)

    江超; 周宜遂

    2009-01-01

    莫西沙星(moxifloxacin,1),化学名为1.环丙基-6-氟-1,4.二氢-8-甲氧基-7-[(4aS,7aS)-八氢.6H-吡咯并[3,4-b]吡啶-6-基]-4-氧代-3-喹啉羧酸,是由德国Bayer公司研发的氟喹诺酮类抗菌药,临床用其盐酸盐。本品于1999年9月首次在德国上市,同年12月在美国上市,用于治疗急性窦腺炎、慢性支气管炎的急性发作、社区获得性肺炎,以及无并发症的皮肤感染和皮肤软组织感染。

  17. Moxifloxacin in Preventing Bacterial Infections in Patients Who Have Undergone Donor Stem Cell Transplant

    Science.gov (United States)

    2013-03-14

    Breast Cancer; Chronic Myeloproliferative Disorders; Gestational Trophoblastic Tumor; Infection; Leukemia; Lymphoma; Multiple Myeloma and Plasma Cell Neoplasm; Myelodysplastic Syndromes; Myelodysplastic/Myeloproliferative Neoplasms; Neuroblastoma; Ovarian Cancer; Testicular Germ Cell Tumor

  18. Population pharmacokinetics and limited sampling strategy for first-line tuberculosis drugs and moxifloxacin

    NARCIS (Netherlands)

    Magis-Escurra, C.; Later-Nijland, H. M. J.; Alffenaar, J. W. C.; Broeders, J.; Burger, D. M.; van Crevel, R.; Boeree, M. J.; Donders, A. R. T.; van Altena, R.; van der Werf, T. S.; Aarnoutse, R. E.

    2014-01-01

    Therapeutic drug monitoring (TDM) of tuberculosis (TB) drugs currently focuses on peak plasma concentrations, yet total exposure [area under the 24-h concentration-time curve (AUC(0-24))] is probably most relevant to the efficacy of these drugs. We therefore assessed population AUC(0-24) data for al

  19. Moxifloxacin pharmacokinetic profile and efficacy evaluation in empiric treatment of community-acquired pneumonia

    DEFF Research Database (Denmark)

    Öbrink-Hansen, Kristina; Hardlei, Tore Forsingdal; Brock, Birgitte;

    2015-01-01

    for each patient were evaluated against epidemiological cutoff MIC values for Streptococcus pneumoniae, Haemophilus influenzae, and Legionella pneumophila. PK-PD targets adopted were a Cmax/MIC of ≥12.2 for all pathogens, an fAUC0-24/MIC of >34 for S. pneumoniae, and an fAUC0-24/MIC of >75 for H....... influenzae and L. pneumophila. Individual predicted estimates for Cmax/MIC and fAUC0-24/MIC as well as simulated maximal MICs resulting in target attainment for oral and intravenous administration of the drug were suitable for S. pneumoniae and H. influenzae but not for L. pneumophila. These results indicate...

  20. Effects of a 7-day continuous infusion of octreotide on circulating levels of growth factors and binding proteins in growth hormone (GH)-treated GH-deficient patients

    DEFF Research Database (Denmark)

    Laursen, Torben; Møller, Jens; Fisker, Sanne;

    1999-01-01

    Abstract In patients with acromegaly, clinical improvement has been reported after octreotide (OCT) treatment, even in cases of only a moderate suppression of growth hormone (GH) levels. In rats, OCT suppresses IGF-I mRNA expression and generation of serum and tissue IGF-I levels. A direct effect...

  1. Validation of the Danish 7-day pre-coded food diary among adults: energy intake v. energy expenditure and recording length

    DEFF Research Database (Denmark)

    Biltoft-Jensen, Anja Pia; Matthiessen, Jeppe; Rasmussen, Lone Banke;

    2009-01-01

    position-and-motion instrument (ActiReg®). Further, the influence of recording length on EI:BMR, percentage consumers, the number of meal occasions and recorded food items per meal was examined. A total of 138 Danish volunteers aged 20–59 years wore the ActiReg® and recorded their food intake for 7...... for EI and EE were − 6·29 and 3·09 MJ/d. Of the participants, 73 % were classified as acceptable reporters, 26 % as under-reporters and 1 % as over-reporters. EI:BMR was significantly lower on 1–3 consecutive recording days compared with 4–7 recording days (P <0·03). Percentage consumers of selected...... beginning and the end of the 7 d reporting. Together, the results indicate that EI was underestimated at group level and that a 7 d recording is preferable to a 4 d recording period....

  2. Relative efficiencies of the Burkard 7-Day, Rotorod and Burkard Personal samplers for Poaceae and Urticaceae pollen under field conditions

    DEFF Research Database (Denmark)

    Peel, Robert George; Kennedy, Roy; Smith, Matt;

    2014-01-01

    Trap, the Sampling Technologies Rotorod Model 20 and the Burkard Personal Volumetric Air Sampler were evaluated for Urticaceae and Poaceae pollen under field conditions, and the influence of wind speed and relative humidity on these efficiency relationships was assessed. Data for the two pollen taxa were...... to only have a significant impact on inter-sampler relationships involving the vertically orientated Burkard Personal sampler, whilst no interaction between relative efficiency and relative humidity was observed. Conclusions: Data collected with the three models of sampler should only be compared once......Introduction: In aerobiological studies it is often necessary to compare concentration data recorded with different models of sampling instrument. Sampler efficiency typically varies from device to device, and depends on the target aerosol and local atmospheric conditions. To account...

  3. Evaluation of 2 × 24-h dietary recalls combined with a food-recording booklet, against a 7-day food-record method among schoolchildren

    DEFF Research Database (Denmark)

    Trolle, Ellen; Amiano, P.; Ege, Majken;

    2011-01-01

    Background/Objectives:The aim of this study was to evaluate the estimated energy, nutrient and food intake from the suggested trans-European methodology for undertaking representative dietary surveys among schoolchildren: 2 Ã 24-h dietary recalls (24-HDRs) combined with a food-recording booklet (...

  4. User experiences of wearable activity monitor among 3-6-year-old preschool children – Are children willing to wear monitor 7 days 24 hours per day?

    Directory of Open Access Journals (Sweden)

    Suvi Määttä

    2015-10-01

    This study was conducted as a part of long-term DAGIS project that aims to improve the health behaviors and diminish socioeconomic inequalities in health behaviors among preschool children in Finland. A large cross-sectional survey is conducted in autumn 2015. Children (N=800, aged 3-6 years wear Actigraph WGT3X-BT accelerometer for seven days, 24 hours per day. Simultaneously with accelerometer use, parents fill in diary with informing the user experiences of accelerometer and possible non-wearing times. Parents are advised that the child wears accelerometer 24 hours and remove the belt only when in water (e.g. in shower. The accelerometer data are checked straight after data collection. Choi (2011 wear time analyses are conducted for data. The device acceptability, compliance for wearing times and reported barriers for using accelerometer 24 hours in seven days among 3-6-year-old children are reported. Conclusions This study provides new information about the usability of wearable activity monitors among 3-6-year-old children, an age group that is less studied.

  5. Investigation of the thrombin-generating capacity, evaluated by thrombogram, and clot formation evaluated by thrombelastography of platelets stored in the blood bank for up to 7 days

    DEFF Research Database (Denmark)

    Johansson, Per Ingemar; Svendsen, M.S.; Salado, J.;

    2008-01-01

    .0035). No correlation between ETP and TTG was found (P = 0.65). CONCLUSION: The kinetics of thrombin generation, as evaluated by CAT, correlates with the thrombus generation, as evaluated by thrombelastography and this may in part explain the clinical utility of the TEG in identifying clinically relevant coagulopathies...

  6. Earthquake prediction in seismogenic areas of the Iberian Peninsula based on computational intelligence

    Science.gov (United States)

    Morales-Esteban, A.; Martínez-Álvarez, F.; Reyes, J.

    2013-05-01

    A method to predict earthquakes in two of the seismogenic areas of the Iberian Peninsula, based on Artificial Neural Networks (ANNs), is presented in this paper. ANNs have been widely used in many fields but only very few and very recent studies have been conducted on earthquake prediction. Two kinds of predictions are provided in this study: a) the probability of an earthquake, of magnitude equal or larger than a preset threshold magnitude, within the next 7 days, to happen; b) the probability of an earthquake of a limited magnitude interval to happen, during the next 7 days. First, the physical fundamentals related to earthquake occurrence are explained. Second, the mathematical model underlying ANNs is explained and the configuration chosen is justified. Then, the ANNs have been trained in both areas: The Alborán Sea and the Western Azores-Gibraltar fault. Later, the ANNs have been tested in both areas for a period of time immediately subsequent to the training period. Statistical tests are provided showing meaningful results. Finally, ANNs were compared to other well known classifiers showing quantitatively and qualitatively better results. The authors expect that the results obtained will encourage researchers to conduct further research on this topic. Development of a system capable of predicting earthquakes for the next seven days Application of ANN is particularly reliable to earthquake prediction. Use of geophysical information modeling the soil behavior as ANN's input data Successful analysis of one region with large seismic activity

  7. Speckle Tracking Based Strain Analysis Is Sensitive for Early Detection of Pathological Cardiac Hypertrophy.

    Directory of Open Access Journals (Sweden)

    Xiangbo An

    Full Text Available Cardiac hypertrophy is a key pathological process of many cardiac diseases. However, early detection of cardiac hypertrophy is difficult by the currently used non-invasive method and new approaches are in urgent need for efficient diagnosis of cardiac malfunction. Here we report that speckle tracking-based strain analysis is more sensitive than conventional echocardiography for early detection of pathological cardiac hypertrophy in the isoproterenol (ISO mouse model. Pathological hypertrophy was induced by a single subcutaneous injection of ISO. Physiological cardiac hypertrophy was established by daily treadmill exercise for six weeks. Strain analysis, including radial strain (RS, radial strain rate (RSR and longitudinal strain (LS, showed marked decrease as early as 3 days after ISO injection. Moreover, unlike the regional changes in cardiac infarction, strain analysis revealed global cardiac dysfunction that affects the entire heart in ISO-induced hypertrophy. In contrast, conventional echocardiography, only detected altered E/E', an index reflecting cardiac diastolic function, at 7 days after ISO injection. No change was detected on fractional shortening (FS, E/A and E'/A' at 3 days or 7 days after ISO injection. Interestingly, strain analysis revealed cardiac dysfunction only in ISO-induced pathological hypertrophy but not the physiological hypertrophy induced by exercise. Taken together, our study indicates that strain analysis offers a more sensitive approach for early detection of cardiac dysfunction than conventional echocardiography. Moreover, multiple strain readouts distinguish pathological cardiac hypertrophy from physiological hypertrophy.

  8. Speckle Tracking Based Strain Analysis Is Sensitive for Early Detection of Pathological Cardiac Hypertrophy.

    Science.gov (United States)

    An, Xiangbo; Wang, Jingjing; Li, Hao; Lu, Zhizhen; Bai, Yan; Xiao, Han; Zhang, Youyi; Song, Yao

    2016-01-01

    Cardiac hypertrophy is a key pathological process of many cardiac diseases. However, early detection of cardiac hypertrophy is difficult by the currently used non-invasive method and new approaches are in urgent need for efficient diagnosis of cardiac malfunction. Here we report that speckle tracking-based strain analysis is more sensitive than conventional echocardiography for early detection of pathological cardiac hypertrophy in the isoproterenol (ISO) mouse model. Pathological hypertrophy was induced by a single subcutaneous injection of ISO. Physiological cardiac hypertrophy was established by daily treadmill exercise for six weeks. Strain analysis, including radial strain (RS), radial strain rate (RSR) and longitudinal strain (LS), showed marked decrease as early as 3 days after ISO injection. Moreover, unlike the regional changes in cardiac infarction, strain analysis revealed global cardiac dysfunction that affects the entire heart in ISO-induced hypertrophy. In contrast, conventional echocardiography, only detected altered E/E', an index reflecting cardiac diastolic function, at 7 days after ISO injection. No change was detected on fractional shortening (FS), E/A and E'/A' at 3 days or 7 days after ISO injection. Interestingly, strain analysis revealed cardiac dysfunction only in ISO-induced pathological hypertrophy but not the physiological hypertrophy induced by exercise. Taken together, our study indicates that strain analysis offers a more sensitive approach for early detection of cardiac dysfunction than conventional echocardiography. Moreover, multiple strain readouts distinguish pathological cardiac hypertrophy from physiological hypertrophy.

  9. Pharmacokinetic/Pharmacodynamic (PK/PD) Indices of Antibiotics Predicted by a Semimechanistic PKPD Model: a Step toward Model-Based Dose Optimization▿

    OpenAIRE

    Nielsen, Elisabet I.; Cars, Otto; Friberg, Lena E.

    2011-01-01

    A pharmacokinetic-pharmacodynamic (PKPD) model that characterizes the full time course of in vitro time-kill curve experiments of antibacterial drugs was here evaluated in its capacity to predict the previously determined PK/PD indices. Six drugs (benzylpenicillin, cefuroxime, erythromycin, gentamicin, moxifloxacin, and vancomycin), representing a broad selection of mechanisms of action and PK and PD characteristics, were investigated. For each drug, a dose fractionation study was simulated, ...

  10. Evaluating the interaction of faecal pellet deposition rates and DNA degradation rates to optimize sampling design for DNA-based mark-recapture analysis of Sonoran pronghorn.

    Science.gov (United States)

    Woodruff, S P; Johnson, T R; Waits, L P

    2015-07-01

    Knowledge of population demographics is important for species management but can be challenging in low-density, wide-ranging species. Population monitoring of the endangered Sonoran pronghorn (Antilocapra americana sonoriensis) is critical for assessing the success of recovery efforts, and noninvasive DNA sampling (NDS) could be more cost-effective and less intrusive than traditional methods. We evaluated faecal pellet deposition rates and faecal DNA degradation rates to maximize sampling efficiency for DNA-based mark-recapture analyses. Deposition data were collected at five watering holes using sampling intervals of 1-7 days and averaged one pellet pile per pronghorn per day. To evaluate nuclear DNA (nDNA) degradation, 20 faecal samples were exposed to local environmental conditions and sampled at eight time points from one to 124 days. Average amplification success rates for six nDNA microsatellite loci were 81% for samples on day one, 63% by day seven, 2% by day 14 and 0% by day 60. We evaluated the efficiency of different sampling intervals (1-10 days) by estimating the number of successful samples, success rate of individual identification and laboratory costs per successful sample. Cost per successful sample increased and success and efficiency declined as the sampling interval increased. Results indicate NDS of faecal pellets is a feasible method for individual identification, population estimation and demographic monitoring of Sonoran pronghorn. We recommend collecting samples >7 days old and estimate that a sampling interval of 4-7 days in summer conditions (i.e. extreme heat and exposure to UV light) will achieve desired sample sizes for mark-recapture analysis while also maximizing efficiency. PMID:25522240

  11. Participant-level meta-analysis of mobile phone-based interventions for smoking cessation across different countries.

    Science.gov (United States)

    Ybarra, Michele L; Jiang, Yannan; Free, Caroline; Abroms, Lorien C; Whittaker, Robyn

    2016-08-01

    With meta-analysis, participant-level data from five text messaging-based smoking cessation intervention studies were pooled to investigate cessation patterns across studies and participants. Individual participant data (N=8315) collected in New Zealand (2001-2003; n=1705), U.K. (2008-2009; n=5792), U.S. (2012; n=503; n=164) and Turkey (2012; n=151) were collectively analyzed in 2014. The primary outcome was self-reported 7-day continuous abstinence at 4weeks post-quit day. Secondary outcomes were: (1) self-reported 7-day continuous abstinence at 3months and (2) self-reported continuous abstinence at 6months post-quit day. Generalized linear mixed models were fit to estimate the overall treatment effect, while accounting for clustering within individual studies. Estimates were adjusted for age, sex, socioeconomic status, previous quit attempts, and baseline Fagerstrom score. Analyses were intention to treat. Participants lost to follow-up were treated as smokers. Twenty-nine percent of intervention participants and 12% of control participants quit smoking at 4weeks (adjusted odds ratio [aOR]=2.89, 95% CI [2.57, 3.26], pcost and scalable programs, along with ongoing evaluation, appear warranted. PMID:27154349

  12. Niobium based coatings for dental implants

    International Nuclear Information System (INIS)

    Niobium based thin films were deposited on stainless steel (SS) substrates to evaluate them as possible biocompatible surfaces that might improve the biocompatibility and extend the life time of stainless steel dental implants. Niobium nitride and niobium oxide thin films were deposited by reactive unbalanced magnetron sputtering under standard deposition conditions without substrate bias or heating. The biocompatibility of the surfaces was evaluated by testing the cellular adhesion and viability/proliferation of human cementoblasts during different culture times, up to 7 days. The response of the films was compared to the bare substrate and pieces of Ti6Al4V; the most commonly used implant material for orthopedics and osteo-synthesis applications. The physicochemical properties of the films were evaluated by different means; X-ray diffraction, Rutherford backscattering spectroscopy and contact angle measurements. The results suggested that the niobium oxide films were amorphous and of stoichiometric Nb2O5 (a-Nb2O5), while the niobium nitride films were crystalline in the FCC phase (c-NbN) and were also stoichiometric with an Nb to N ratio of one. The biological evaluation showed that the biocompatibility of the SS could be improved by any of the two films, but neither was better than the Ti6Al4V alloy. On the other hand, comparing the two films, the c-NbN seemed to be a better surface than the oxide in terms of the adhesion and proliferation of human cemetoblasts.

  13. Niobium based coatings for dental implants

    Science.gov (United States)

    Ramírez, G.; Rodil, S. E.; Arzate, H.; Muhl, S.; Olaya, J. J.

    2011-01-01

    Niobium based thin films were deposited on stainless steel (SS) substrates to evaluate them as possible biocompatible surfaces that might improve the biocompatibility and extend the life time of stainless steel dental implants. Niobium nitride and niobium oxide thin films were deposited by reactive unbalanced magnetron sputtering under standard deposition conditions without substrate bias or heating. The biocompatibility of the surfaces was evaluated by testing the cellular adhesion and viability/proliferation of human cementoblasts during different culture times, up to 7 days. The response of the films was compared to the bare substrate and pieces of Ti6Al4V; the most commonly used implant material for orthopedics and osteo-synthesis applications. The physicochemical properties of the films were evaluated by different means; X-ray diffraction, Rutherford backscattering spectroscopy and contact angle measurements. The results suggested that the niobium oxide films were amorphous and of stoichiometric Nb2O5 (a-Nb2O5), while the niobium nitride films were crystalline in the FCC phase (c-NbN) and were also stoichiometric with an Nb to N ratio of one. The biological evaluation showed that the biocompatibility of the SS could be improved by any of the two films, but neither was better than the Ti6Al4V alloy. On the other hand, comparing the two films, the c-NbN seemed to be a better surface than the oxide in terms of the adhesion and proliferation of human cemetoblasts.

  14. OCT-based label-free in vivo lymphangiography within human skin and areola

    Science.gov (United States)

    Baran, Utku; Qin, Wan; Qi, Xiaoli; Kalkan, Goknur; Wang, Ruikang K.

    2016-02-01

    Due to the limitations of current imaging techniques, visualization of lymphatic capillaries within tissue in vivo has been challenging. Here, we present a label-free high resolution optical coherence tomography (OCT) based lymphangiography (OLAG) within human skin in vivo. OLAG enables rapid (~seconds) mapping of lymphatic networks, along with blood vessel networks, over 8 mm x 8 mm of human skin and 5 mm x 5 mm of human areola. Moreover, lymphatic system’s response to inflammation within human skin is monitored throughout an acne lesion development over 7 days. The demonstrated results promise OLAG as a revolutionary tool in the clinical research and treatment of patients with pathologic conditions such as cancer, diabetes, and autoimmune diseases.

  15. Tumors in murine brains studied by grating-based phase contrast microtomography

    Science.gov (United States)

    Schulz, Georg; Dominietto, Marco; Kovacs, Zsofia; Schmitz, Rüdiger; Hieber, Simone E.; Thalmann, Peter; Beckmann, Felix; Müller, Bert

    2014-09-01

    Angiogenesis, i.e. the formation of vessels, is one of the key processes during tumor development. The newly formed vessels transport oxygen and nutrients from the healthy tissue to the tumor and gives tumor cells the possibility to replicate. The principle of anti-angiogenic therapy is to block angiogenic process in order to stop tumor growth. The aim of the present study is the investigation of murine glioma vascular architecture at early (7 days), intermediate (10 and 15 days) and late (23 days) stage of growth by means of grating-based phase contrast microtomography. We demonstrate that this technique yields premium contrast between healthy and cancerous parts of murine brain tissues.

  16. Sonochemical processing and characterization of composite materials based on soy protein and alginate containing micron-sized bioactive glass particles

    Science.gov (United States)

    Silva, Raquel; Bulut, Buse; Roether, Judith A.; Kaschta, Joachim; Schubert, Dirk W.; Boccaccini, Aldo R.

    2014-09-01

    Novel composite hydrogels based on the combination of natural polymers; namely alginate and soy protein isolate, and bioactive glass (BG) particles (mean size: 2 μm) were developed. For this purpose a sonochemical approach was used and homogeneous composite hydrogels, incorporating two concentrations of BG particles, were successfully obtained. Further physico-chemical characterization was performed in order to evaluate the influence of each component on hydrogel properties. The water uptake ability, weight loss, protein release, as well as FTIR, SEM and DMTA characterization were carried out. The biomineralization process in simulated body fluid (SBF) was followed over time and the results demonstrated that the composite materials have the ability to form a surface apatite layer after 7 days in SBF. The design of novel composite hydrogels based on soy protein, alginate and BG can be a suitable approach for bone regeneration applications.

  17. Mechanical properties of gangue-containing aluminosilicate based cementitious materials

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    High performance aluminosilicate based cementitious materials were produced using calcined gangue as one of the major raw materials.The gangue was calcined at 500℃.The main constituent was calcined gangue, fly ash and slag, while alkali-silicate solutions were used as the diagenetic agent.The structure of gangue-containing aluminosilicate based cementitious materials was studied by the methods of IR, NMR and SEM.The results show that the mechanical properties are affected by the mass ratio between the gangue, slag and fly ash, the kind of activator and additional salt.For 28-day curing time, the compressive strength of the sample with a mass proportion of 2:1:1 (gangue: slag: fly ash) is 58.9 MPa, while the compressive strength of the sample containing 80wt%gangue can still be up to 52.3 MPa.The larger K+ favors the formation of large silicate oligomers with which Al(OH)4- prefers to bind.Therefore, in Na-K compounding activator solutions more oligomers exist which result in a stronger compressive strength of aluminosilicate-based cementitious materials than in the case of Na-containing activator.The reasons for this were found through IR and NMR analysis.Glauber's salt reduces the 3-day compressive strength of the paste, but increases its 7-day and 28-day compressive strengths.

  18. Covariate analysis of QTc and T-wave morphology: new possibilities in the evaluation of drugs that affect cardiac repolarization

    DEFF Research Database (Denmark)

    Graff, Claus; Struijk, Johannes J.; Matz, J;

    2010-01-01

    This study adds the dimension of a T-wave morphology composite score (MCS) to the QTc interval-based evaluation of drugs that affect cardiac repolarization. Electrocardiographic recordings from 62 subjects on placebo and 400 mg moxifloxacin were compared with those from 21 subjects on 160 and 320...

  19. Chronic respiratory symptoms, bronchial responsiveness and dietary sodium and potassium: a population-based study.

    Science.gov (United States)

    Zoia, M C; Fanfulla, F; Bruschi, C; Basso, O; De Marco, R; Casali, L; Cerveri, I

    1995-04-01

    A possible relationship between Na+ intake and increased prevalence and mortality from asthma and chronic obstructive pulmonary disease (COPD) has been suggested but not clearly proven for several reasons (difficulty in assessing Na+ and K+ both by 24 h excretion and dietary recall, too small an effect of these ions on the pathology, and the role of potential confounders). We wanted to determine the relationship of Na+ and K+ intake, assessed by means of a 7 day recall, with chronic respiratory symptoms and bronchial responsiveness in a sample of the general population. Two hundred and five subjects were studied, with complete dietary and respiratory questionnaires, and baseline respiratory function tests, together with a subsample of 146 subjects who underwent histamine challenge. The 7 day recall consisted of two parts: the first assessed discretionary Na+; and the second assessed Na+ and K+ contained in food. The whole sample was split into two groups based on the levels of consumption, and the statistical analysis was performed contrasting the three lower quartiles vs the highest. Smoking habit, social economic status, age and body mass index (BMI) were not confounders for Na+ and K+ intake. The prevalence of symptomatic subjects and baseline respiratory function values were not significantly different in the two groups of quartiles for Na+ and K+. Baseline respiratory values and dose-response slope of the subsample were also not significantly different. We did not prove a relationship between these dietary factors and either bronchial responsiveness or chronic respiratory symptoms. Although we consider that our questionnaire is more reliable than other methods for Na+ and K+ assessment, several potential biases still remain.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:7613539

  20. Bioactivity of polyurethane-based scaffolds coated with Bioglass (registered)

    International Nuclear Information System (INIS)

    Polyurethane (PUR) and polyurethane/poly(d, l-lactide) acid (PUR/PDLLA) based scaffolds coated with Bioglass (registered) particles for application in bone tissue engineering were fabricated. The slurry-dipping method was used for coating preparation. The homogeneous structure of the Bioglass (registered) coatings on the surface of the PUR and PUR/PDLLA foams indicated a good adhesion of the bioactive glass particles to polyurethane without any additional surface treatment. In vitro studies in simulated body fluid (SBF) were performed to study the influence of Bioglass (registered) coating on biodegrability and bioactivity of PUR-based scaffolds. The surface of Bioglass (registered) -coated samples was covered by a layer of carbonate-containing apatite after 7 days of immersion in SBF, while in uncoated polymer samples apatite crystals were not detected even after 21 days of immersion in SBF. The apatite layer was characterized by scanning electron microscopy (SEM), EDS analysis and attenuated total reflectance-Fourier transform infrared spectrometry (FTIR-ATR). Weight loss measurements showed that the in vitro degradation rate of the composite scaffolds in SBF was higher in comparison to uncoated polyurethane samples. PUR and PUR/PDLLA foams with Bioglass (registered) coating have potential to be used as bioactive, biodegradable scaffolds in bone tissue engineering

  1. Alginate/chitosan based bi-layer composite membrane as potential sustained-release wound dressing containing ciprofloxacin hydrochloride

    Science.gov (United States)

    Han, Fei; Dong, Yang; Song, Aihua; Yin, Ran; Li, Sanming

    2014-08-01

    The aims of this research were to develop and evaluate a novel ciprofloxacin hydrochloride loaded bi-layer composite membrane based on alginate and chitosan. In vitro antimicrobial activity, drug permeation study, morphology, cytotoxicity, primary skin irritation and in vivo pharmacodynamics were investigated. Results showed that the membranes could inhibit the growth of microorganisms for longer than 7 days. And there was no significant decrease in the metabolic activity of the Hacat fibroblasts cells were treated with the membranes. No edema and erythema were observed after administration of membranes on the rabbit skin after 14 days. Moreover, the results of pharmacodynamics showed that the membranes were more effective in improving the wound healing process. In conclusion, a novel bi-layer composite membrane was developed and results suggested that it could be exploited as sustained-release wound dressings.

  2. Caregiver Training in Mindfulness-Based Positive Behavior Supports (MBPBS): Effects on Caregivers and Adults with Intellectual and Developmental Disabilities.

    Science.gov (United States)

    Singh, Nirbhay N; Lancioni, Giulio E; Karazsia, Bryan T; Myers, Rachel E

    2016-01-01

    Caregivers often manage the aggressive behavior of individuals with intellectual and developmental disabilities that reside in community group homes. Sometimes this results in adverse outcomes for both the caregivers and the care recipients. We provided a 7-day intensive Mindfulness-Based Positive Behavior Support (MBPBS) training to caregivers from community group homes and assessed the outcomes in terms of caregiver variables, individuals' behaviors, and an administrative outcome. When compared to pre-MBPBS training, the MBPBS training resulted in the caregivers using significantly less physical restraints, and staff stress and staff turnover were considerably reduced. The frequency of injury to caregivers and peers caused by the individuals was significantly reduced. A benefit-cost analysis showed substantial financial savings due to staff participation in the MBPBS program. This study provides further proof-of-concept for the effectiveness of MBPBS training for caregivers, and strengthens the call for training staff in mindfulness meditation. PMID:26903906

  3. Caregiver Training in Mindfulness-Based Positive Behavior Supports (MBPBS: Effects on Caregivers and Adults with Intellectual and Developmental Disabilities

    Directory of Open Access Journals (Sweden)

    Nirbhay N. Singh

    2016-02-01

    Full Text Available Caregivers often manage the aggressive behavior of individuals with intellectual and developmental disabilities that reside in community group homes. Sometimes this results in adverse outcomes for both the caregivers and the care recipients. We provided a 7-day intensive Mindfulness-Based Positive Behavior Support (MBPBS training to caregivers from community group homes and assessed the outcomes in terms of caregiver variables, individuals’ behaviors, and an administrative outcome. When compared to pre-MBPBS training, the training resulted in the caregivers using significantly less physical restraints, and staff stress and staff turnover were considerably reduced. The frequency of injury to caregivers and peers caused by the individuals was significantly reduced. A benefit-cost analysis showed substantial financial savings due to staff participation in the MBPBS program. This study provides further proof of concept for the effectiveness of MBPBS training for caregivers, and strengthens the call for training staff in mindfulness meditation.

  4. Resistance-Associated NS5A Variants of Hepatitis C Virus Are Susceptible to Interferon-Based Therapy.

    Directory of Open Access Journals (Sweden)

    Jun Itakura

    Full Text Available The presence of resistance-associated variants (RAVs of hepatitis C virus (HCV attenuates the efficacy of direct acting antivirals (DAAs. The objective of this study was to characterize the susceptibility of RAVs to interferon-based therapy.Direct and deep sequencing were performed to detect Y93H RAV in the NS5A region. Twenty nine genotype 1b patients with detectable RAV at baseline were treated by a combination of simeprevir, pegylated interferon and ribavirin. The longitudinal changes in the proportion of Y93H RAV during therapy and at breakthrough or relapse were determined.By direct sequencing, Y93H RAV became undetectable or decreased in proportion at an early time point during therapy (within 7 days in 57% of patients with both the Y93H variant and wild type virus at baseline when HCV RNA was still detectable. By deep sequencing, the proportion of Y93H RAV against Y93 wild type was 52.7% (5.8%- 97.4% at baseline which significantly decreased to 29.7% (0.16%- 98.3% within 7 days of initiation of treatment (p = 0.023. The proportion of Y93H RAV was reduced in 21 of 29 cases (72.4% and a marked reduction of more than 10% was observed in 14 cases (48.7%. HCV RNA reduction was significantly greater for Y93H RAV (-3.65±1.3 logIU/mL/day than the Y93 wild type (-3.35±1.0 logIU/mL/day (p<0.001.Y93H RAV is more susceptible to interferon-based therapy than the Y93 wild type.

  5. Development of dietary-based toxic reference values to assess the risk of chlorophacinone to non-target raptorial birds

    Science.gov (United States)

    Rattner, Barnett A.; Lazarus, Rebecca; Shultz, S. L.; Horak, Katherine E.; Abbo, B.G.; Volker, Steven F.

    2014-01-01

    Regulatory changes in the use of some second-generation anticoagulant rodenticides in parts of North America may result in expanded use of first-generation anticoagulant rodenticides (FGARs). Recent toxicological studies with captive raptors have demonstrated that these species are considerably more sensitive to the FGAR diphacinone than traditional avian wildlife test species (mallard, bobwhite). We have now examined the toxicity of the FGAR chlorophacinone (CPN) to American kestrels fed rat tissue mechanically-amended with CPN, or rat tissue containing biologically-incorporated CPN, for 7 days. Nominal CPN concentrations in these diets were 0.15, 0.75 and 1.5 µg/g food wet weight, and actual CPN concentration in diets were analytically verified as being close to target values. Food intake was consistent among groups, body weight fluctuated by less than 6%, exposure and adverse effects were generally dose-dependent, and there were no dramatic differences in toxicity between mechanically-amended and biologically-incorporated CPN diets. Using benchmark dose statistical methods, toxic reference values at which clotting times were prolonged in 50% of the kestrels was estimated to be about 80 µg CPN consumed/kg body weight-day for prothrombin time and 40 µg CPN/kg body weight-day for Russell's viper venom time. Based upon carcass CPN residues reported in rodents from field baiting studies, empirical measures of food consumption in kestrels, and dietary-based toxic reference values derived from the 7-day exposure scenario, some free-ranging raptors consuming CPN exposed prey might exhibit coagulopathy and hemorrhage. These sublethal responses associated with exposure to environmentally realistic concentrations of CPN could compromise survival of exposed birds.

  6. Niobium based coatings for dental implants

    Energy Technology Data Exchange (ETDEWEB)

    Ramirez, G., E-mail: enggiova@hotmail.com [Instituto de Investigaciones en Materiales, Universidad Nacional Autonoma de Mexico, Circuito Exterior s/n, CU, Mexico D.F. 04510 (Mexico); Facultad de Quimica, Departamento de Ingenieria Quimica, Universidad Nacional Autonoma de Mexico, Mexico D.F. 04510 (Mexico); Rodil, S.E. [Instituto de Investigaciones en Materiales, Universidad Nacional Autonoma de Mexico, Circuito Exterior s/n, CU, Mexico D.F. 04510 (Mexico); Arzate, H. [Laboratorio de Biologia Celular y Molecular, Facultad de Odontologia, Universidad Nacional Autonoma de Mexico, CU, Mexico D.F. 04510 (Mexico); Muhl, S. [Instituto de Investigaciones en Materiales, Universidad Nacional Autonoma de Mexico, Circuito Exterior s/n, CU, Mexico D.F. 04510 (Mexico); Olaya, J.J. [Unidad de Materiales, Departamento de Ingenieria Mecanica y Mecatronica, Universidad Nacional de Colombia, Cra. 30 45-03 Bogota (Colombia)

    2011-01-15

    Niobium based thin films were deposited on stainless steel (SS) substrates to evaluate them as possible biocompatible surfaces that might improve the biocompatibility and extend the life time of stainless steel dental implants. Niobium nitride and niobium oxide thin films were deposited by reactive unbalanced magnetron sputtering under standard deposition conditions without substrate bias or heating. The biocompatibility of the surfaces was evaluated by testing the cellular adhesion and viability/proliferation of human cementoblasts during different culture times, up to 7 days. The response of the films was compared to the bare substrate and pieces of Ti6Al4V; the most commonly used implant material for orthopedics and osteo-synthesis applications. The physicochemical properties of the films were evaluated by different means; X-ray diffraction, Rutherford backscattering spectroscopy and contact angle measurements. The results suggested that the niobium oxide films were amorphous and of stoichiometric Nb{sub 2}O{sub 5} (a-Nb{sub 2}O{sub 5}), while the niobium nitride films were crystalline in the FCC phase (c-NbN) and were also stoichiometric with an Nb to N ratio of one. The biological evaluation showed that the biocompatibility of the SS could be improved by any of the two films, but neither was better than the Ti6Al4V alloy. On the other hand, comparing the two films, the c-NbN seemed to be a better surface than the oxide in terms of the adhesion and proliferation of human cemetoblasts.

  7. Radiocalcium Measurement of Bone Turnover in Disorders of Calcium Metabolism Using a Model Based on an Expanding Pool

    International Nuclear Information System (INIS)

    To determine bone mineralization rate the patient eats a constant daily diet for the duration of the study. 10 μCi of 47CaCl2 are injected intravenously on day one of the investigation. Blood samples are taken at 5, 10, 20, 40 and 90 minutes, 3, 5, 9 and 22 hours and 2, 3, 4, 5 and 7 days. 47Ca radioactivity is measured in all the plasma and daily faecal and urine samples. Bone mineralization is determined using a model based on the concept that the exchangeable calcium pool is continuously expanding according to a power function and that activity is lost from the pool into faeces, urine and bone (by mineralization) in direct proportion to the specific activity of the plasma (S*). The power function can be determined graphically from a log/log plot of the S* data over the first 15 hours. Extrapolation of this line to day 7 permits calculation of the pool size at that time (reciprocal of the calculated Sp*7) and the product of the calculated pool and actual S7* yields the amount of activity in the pool at day 7. This is deducted from the activity retained at day 7 to give the activity attributable to bone mineralization which is then divided by the integrated S* over the 7 days to yield the bone mineralization rate. Usually the whole calculation is performed by curve fitting the data points with the aid of a computer. In contrast to previously published methods for the determination of the bone mineralization rates the results presented are independent of the portion of the S* curve that is analysed and thus bone mineralization can be determined in 7 days, previous methods requiring 14 days or more. The normal mineralization rate is 4-6 mg Ca/kg per day. High values of bone mineralization are found in groups of patients with Paget' s disease, osteomalacia, hyperthyroidism, hyperparathyroidism and renal osteodystrophy. Low values for bone mineralization have been found in some patients with crush fracture osteoporosis and in hypoparathyroidism. Changes in bone

  8. Web-based data delivery services in support of disaster-relief applications

    Science.gov (United States)

    Jones, B.K.; Risty, R.R.; Buswell, M.

    2003-01-01

    The U.S. Geological Survey Earth Resources Observation Systems Data Center responds to emergencies in support of various government agencies for human-induced and natural disasters. This response consists of satellite tasking and acquisitions, satellite image registrations, disaster-extent maps analysis and creation, base image provision and support, Web-based mapping services for product delivery, and predisaster and postdisaster data archiving. The emergency response staff are on call 24 hours a day, 7 days a week, and have access to many commercial and government satellite and aerial photography tasking authorities. They have access to value-added data processing and photographic laboratory services for off-hour emergency requests. They work with various Federal agencies for preparedness planning, which includes providing base imagery. These data may include digital elevation models, hydrographic models, base satellite images, vector data layers such as roads, aerial photographs, and other predisaster data. These layers are incorporated into a Web-based browser and data delivery service that is accessible either to the general public or to select customers. As usage declines, the data are moved to a postdisaster nearline archive that is still accessible, but not in real time.

  9. Successful alternative treatment of extensively drug-resistant tuberculosis in Argentina with a combination of linezolid, moxifloxacin and thioridazine.

    NARCIS (Netherlands)

    Abbate, E.; Vescovo, M.; Natiello, M.; Cufre, M.; Garcia, A.; Gonzalez Montaner, P.; Ambroggi, M.; Ritacco, V.; Soolingen, D. van

    2012-01-01

    OBJECTIVES: Current drug choices to treat extensively drug-resistant (XDR) tuberculosis (TB) are scarce; therefore, information on the safety, tolerability and efficacy of alternative regimens is of utmost importance. The aim of this study was to describe the management, drug adverse effects and out

  10. Moxifloxacin hydrochloride-induced visual hallucinations%盐酸莫西沙星导致视幻觉

    Institute of Scientific and Technical Information of China (English)

    刘辉; 孙锋钢

    2008-01-01

    1名47岁女性患者,因泌尿系感染服用盐酸莫西沙星400 mg,2~3 h后患者出现恶心和多汗,5~6 h后发生视幻觉,眼前出现人和动物,持续3 h左右.次日再服同剂量莫西沙星2 h后,又出现上述视幻觉.脑电图示较多的β快波节律.停服莫西沙星并予对症治疗,症状消失.半年后随访,无神经系统症状、体征,脑电图基本正常.

  11. Effect of discarded keratin-based biocomposite hydrogels on the wound healing process in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Park, Mira [Department of Organic Materials & Fiber Engineering, Chonbuk National University, Jeonju 561–756 (Korea, Republic of); Shin, Hye Kyoung [Department of Chemistry, Inha University, 100 Inharo, Incheon 402–751 (Korea, Republic of); Kim, Byoung-Suhk [Department of BIN fusion technology, Chonbuk National University, Jeonju 561–756 (Korea, Republic of); Kim, Myung Jin; Kim, In-Shik [Department of Veterinary Anatomy, College of Veterinary Medicine and Bio-safety Research institute, Chonbuk National University, Jeonju 561–756 (Korea, Republic of); Park, Byung-Yong, E-mail: parkb@jbnu.ac.kr [Department of Veterinary Anatomy, College of Veterinary Medicine and Bio-safety Research institute, Chonbuk National University, Jeonju 561–756 (Korea, Republic of); Kim, Hak-Yong, E-mail: khy@jbnu.ac.kr [Department of BIN fusion technology, Chonbuk National University, Jeonju 561–756 (Korea, Republic of)

    2015-10-01

    Biocompatible keratin-based hydrogels prepared by electron beam irradiation (EBI) were examined in wound healing. As the EBI dose increased to 60 kGy, the tensile strength of the hydrogels increased, while the percentage of elongation of the hydrogels decreased. After 7 days, the dehydrated wool-based hydrogels show the highest mechanical properties (the % elongation of 1341 and the tensile strength of 6030 g/cm{sup 2} at an EBI dose of 30 kGy). Excision wound models were used to evaluate the effects of human hair-based hydrogels and wool-based hydrogels on various phases of healing. On post-wounding days 7 and 14, wounds treated with either human hair-based or wool-based hydrogels were greatly reduced in size compared to wounds that received other treatments, although the hydrocolloid wound dressing-treated wound also showed a pronounced reduction in size compared to an open wound as measured by a histological assay. On the 14th postoperative day, the cellular appearances were similar in the hydrocolloid wound dressing and wool-based hydrogel-treated wounds, and collagen fibers were substituted with fibroblasts and mixed with fibroblasts in the dermis. Furthermore, the wound treated with a human hair-based hydrogel showed almost complete epithelial regeneration, with the maturation of immature connective tissue and hair follicles and formation of a sebaceous gland. - Highlights: • Biocompatible keratin-based hydrogels were examined for wound healing process. • Human hair-based hydrogel is superior to wool-based hydrogel in wound healing. • Discarded keratin-based hydrogels are expected more eco-friendly therapeutic agents.

  12. SOL-GEL SILICA-BASED Ag–Ca–P COATINGS WITH AGRESSIVE PRETREATMENT OF TITANIUM SUBSTRATE

    Directory of Open Access Journals (Sweden)

    ELENA BORSHCHEVA

    2011-12-01

    Full Text Available The aim of the experiment was the obtaining of thin silica coatings on titanium by sol-gel method, using mechanical (SiC - paper No.180 and chemical (leaching in HF pretreatments of the titanium substrates. The solutions were based on TEOS. For the sol-gel dipping process 4 different solutions were prepared: silica, silica with AgNO3 and silica + AgNO3 with brushite (CaHPO4·2H2O or monetite (CaHPO4 powders. The solutions were aged for 7 and 14 days at laboratory temperature. After sol-gel dip-coating process the samples were dried and fired. The adhesion of fired coatings was measured by tape test according to ASTM procedure and the bioactivity of the coatings was tested using in vitro test. The surfaces of the samples after firing, tape test and in vitro test were observed with the optical and electron microscopes. The firing results showed that silica-silver coatings did not change, brushite sol-gel coatings have cracked and the monetite sol-gel coatings have cracked also, but less than brushite ones. In spite of coating´s crackings, the square’s frames made on the surfaces were without any breakdowns after tape tests and the adhesion of all coatings was very good, classified by the highest grade 5. The results of in vitro tests showed that all coatings interacted with simulated body fluid (SBF. After exposition in SBF the new layer formed on substrates. In case of 7 days aged coatings containing brushite the new layer was uniform and compact. In case of 7 days aged coatings containing monetite the new layer was formed by crystals aggregated tightly together. The monetite and brushite coatings prepared from 14 days aged sol were the same as previous ones, but they were thicker. X-ray analyses after in vitro test confirmed dellaite, titanate and hydroxyapatite phases.

  13. Efficacy of rifabutin-based triple therapy as second-line treatment to eradicate helicobacter pylori infection

    Directory of Open Access Journals (Sweden)

    Méndez Isabel

    2007-07-01

    Full Text Available Abstract Background Rifabutin has been found to be effective in multi-resistant patients after various treatment cycles for Helicobacter pylori (HP infection, but it has not been analysed as a second-line treatment. Therefore, we seek to compare the effectiveness of a treatment regimen including rifabutin versus conventional quadruple therapy (QT. Methods Open clinical trial, randomised and multi-centre, of two treatment protocols: A Conventional regime -QT- (omeprazole 20 mg bid, bismuth citrate 120 mg qid, tetracycline 500 mg qid and metronidazole 500 mg tid; B Experimental one -OAR- (omeprazole 20 mg bid, amoxicillin 1 gr bid, and rifabutin 150 mg bid, both taken orally for 7 days, in patients with HP infection for whom first-line treatment had failed. Eradication was determined by Urea Breath Test (UBT. Safety was determined by the adverse events. Results 99 patients were randomised, QT, n = 54; OAR, n = 45. The two groups were homogeneous. In 8 cases, treatment was suspended (6 in QT and 2 in OAR. The eradication achieved, analysed by ITT, was for QT, 38 cases (70.4%, and for OAR, 20 cases (44.4%; p = 0.009, OR = 1.58. Of the cases analysed PP, QT were 77.1%; OAR, 46.5%; p = 0.002. Adverse effects were described in 64% of the QT patients and in 44% of the OAR patients (p = 0.04. Conclusion A 7-day rifabutin-based triple therapy associated to amoxicillin and omeprazole at standard dose was not found to be effective as a second-line rescue therapy. The problem with quadruple therapy lies in the adverse side effects it provokes. We believe the search should continue for alternatives that are more comfortably administered and that are at least as effective, but with fewer adverse side effects. Trial Registration Current Controlled Trials ISRCTN81058036

  14. Formation of Porous Apatite Layer during In Vitro Study of Hydroxyapatite-AW Based Glass Composites

    Directory of Open Access Journals (Sweden)

    Pat Sooksaen

    2015-01-01

    Full Text Available This research discussed the fabrication, characterization, and in vitro study of composites based on the mixture of hydroxyapatite powder and apatite-wollastonite (AW based glass. AW based glass was prepared from the SiO2-CaO-MgO-P2O5-CaF2 glass system. This study focuses on the effect of composition and sintering temperature that influences the properties of these composites. Microstructural study revealed the formation of apatite layer on the composite surfaces when immersed in simulated body fluid (SBF solution at 37°C. Composites containing ≥50 wt% AW based glass showed good bioactivity after 7 days of immersion in the SBF. A porous calcium phosphate (potentially hydroxycarbonate apatite, HCA layer formed at the SBF-composite interface and the layer became denser at longer soaking period, for periods ranging from 7 to 28 days. Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES analysis showed that early stage of soaking occurred with the release of Ca and Si ions from the composites and the decrease of P ions with slow exchange rate.

  15. Testing Game-Based Performance in Team-Handball.

    Science.gov (United States)

    Wagner, Herbert; Orwat, Matthias; Hinz, Matthias; Pfusterschmied, Jürgen; Bacharach, David W; von Duvillard, Serge P; Müller, Erich

    2016-10-01

    Wagner, H, Orwat, M, Hinz, M, Pfusterschmied, J, Bacharach, DW, von Duvillard, SP, and Müller, E. Testing game-based performance in team-handball. J Strength Cond Res 30(10): 2794-2801, 2016-Team-handball is a fast paced game of defensive and offensive action that includes specific movements of jumping, passing, throwing, checking, and screening. To date and to the best of our knowledge, a game-based performance test (GBPT) for team-handball does not exist. Therefore, the aim of this study was to develop and validate such a test. Seventeen experienced team-handball players performed 2 GBPTs separated by 7 days between each test, an incremental treadmill running test, and a team-handball test game (TG) (2 × 20 minutes). Peak oxygen uptake (V[Combining Dot Above]O2peak), blood lactate concentration (BLC), heart rate (HR), sprinting time, time of offensive and defensive actions as well as running intensities, ball velocity, and jump height were measured in the game-based test. Reliability of the tests was calculated using an intraclass correlation coefficient (ICC). Additionally, we measured V[Combining Dot Above]O2peak in the incremental treadmill running test and BLC, HR, and running intensities in the team-handball TG to determine the validity of the GBPT. For the test-retest reliability, we found an ICC >0.70 for the peak BLC and HR, mean offense and defense time, as well as ball velocity that yielded an ICC >0.90 for the V[Combining Dot Above]O2peak in the GBPT. Percent walking and standing constituted 73% of total time. Moderate (18%) and high (9%) intensity running in the GBPT was similar to the team-handball TG. Our results indicated that the GBPT is a valid and reliable test to analyze team-handball performance (physiological and biomechanical variables) under conditions similar to competition.

  16. Designing a Sustainable Noise Mapping System Based on Citizen Scientists Smartphone Sensor Data

    Science.gov (United States)

    Shim, Eunyoung; Kim, Dohyeong; Woo, Hyekyung; Cho, Youngtae

    2016-01-01

    In this study, we attempted to assess the feasibility of collecting population health data via mobile devices. Specifically, we constructed noise maps based on sound information monitored by individuals’ smartphones. We designed a sustainable way of creating noise maps that can overcome the shortcomings of existing station-based noise-monitoring systems. Three hundred and nine Seoul residents aged 20–49 years who used Android-based smartphones were recruited, and the subjects installed a special application that we developed for this study. This application collected information on sound and geographical location every 10 min for 7 days. Using GIS, we were able to construct various types of noise maps of Seoul (e.g., daytime/nighttime and weekdays/weekends) using the information on sound and geographical location obtained via the users’ smartphones. Despite the public health importance of noise management, a number of countries and cities lack a sustainable system to monitor noise. This pilot study showed the possibility of using the smartphones of citizen scientists as an economical and sustainable way of monitoring noise, particularly in an urban context in developing countries. PMID:27626273

  17. Designing a Sustainable Noise Mapping System Based on Citizen Scientists Smartphone Sensor Data.

    Science.gov (United States)

    Shim, Eunyoung; Kim, Dohyeong; Woo, Hyekyung; Cho, Youngtae

    2016-01-01

    In this study, we attempted to assess the feasibility of collecting population health data via mobile devices. Specifically, we constructed noise maps based on sound information monitored by individuals' smartphones. We designed a sustainable way of creating noise maps that can overcome the shortcomings of existing station-based noise-monitoring systems. Three hundred and nine Seoul residents aged 20-49 years who used Android-based smartphones were recruited, and the subjects installed a special application that we developed for this study. This application collected information on sound and geographical location every 10 min for 7 days. Using GIS, we were able to construct various types of noise maps of Seoul (e.g., daytime/nighttime and weekdays/weekends) using the information on sound and geographical location obtained via the users' smartphones. Despite the public health importance of noise management, a number of countries and cities lack a sustainable system to monitor noise. This pilot study showed the possibility of using the smartphones of citizen scientists as an economical and sustainable way of monitoring noise, particularly in an urban context in developing countries.

  18. Virological failure of staggered and simultaneous treatment interruption in HIV patients who began Efavirenz-based regimens after allergic reactions to nevirapine

    Directory of Open Access Journals (Sweden)

    Siripassorn Krittaecho

    2013-01-01

    Full Text Available Abstract Objective The objective of this work was to study the virological outcomes associated with two different types of treatment interruption strategies in patients with allergic reactions to nevirapine (NVP. We compared the virological outcomes of (1 HIV-1-infected patients who discontinued an initial NVP-based regimen because of cutaneous allergic reactions to NVP; different types of interruption strategies were used, and second-line regimen was based on efavirenz (EFV; and (2 HIV-1-infected patients who began an EFV-based regimen as a first-line therapy (controls. Methods This retrospective cohort included patients who began an EFV-based regimen, between January 2002 and December 2008, as either an initial regimen or as a subsequent regimen after resolving a cutaneous allergic reaction against an initial NVP-based regimen. The study ended in March 2010. The primary outcome was virological failure, which was defined as either (a two consecutive plasma HIV-1 RNA levels >400 copies/mL or (b a plasma HIV-1 RNA level >1,000 copies/mL plus any genotypic resistance mutation. Results A total of 559 patients were stratified into three groups: (a Simultaneous Interruption, in which the subjects simultaneously discontinued all the drugs in an NVP-based regimen following an allergic reaction (n=161; (b Staggered Interruption, in which the subjects discontinued NVP treatment while continuing nucleoside reverse transcriptase inhibitor (NRTI backbone therapy for a median of 7 days (n=82; and (c Control, in which the subjects were naïve to antiretroviral therapy (n=316. The overall median follow-up time was 43 months. Incidence of virological failure in Simultaneous Interruption was 12.9 cases per 1,000 person-years, which trended toward being higher than the incidences in Staggered Interruption (5.4 and Control (6.6. However, differences were not statistically significant. Conclusions Among the patients who had an acute allergic reaction to first

  19. Accurate diagnoses, evidence based drugs, and new devices (3 Ds in heart failure

    Directory of Open Access Journals (Sweden)

    Bambang B. Siswanto

    2012-02-01

    Full Text Available Heart failure becomes main problem in cardiology because of increasing of heart failure patients, rehospitalization rate, morbidity, and mortality rate. The main causes of increasing heart failure problems are: (1 Successful treatment of acute myocardial infarction can be life saving, but its sequelae can cause heart failure. (2 Increasing life expectancy rate grows along with incidences of ageing related heart failure. (3 High prevalence of infection in Indonesia can cause rheumatic heart disease post Streptococcal beta hemolyticus infection, viral myocarditis, infective endocartitis, and tuberculoid pericarditis. (4 Many risk factors for coronary heart disease are often found in heart failure patients, for examples smoking, diabetes, hypercholesterolemia, hypertension, and obesity. Indonesia joined international multicentered registry in 2006. Acute Decompensated HEart failure REgistry is a web based international registry to record patient with acute decompensated heart failure treated in emergency room. It was found that heart failure patients in 5 big hospitals in Java and Bali island that joined this registry are younger, sicker and late to seek treatment. The median hospital length of stay was 7 days and in hospital mortality rate was 6.7%. The aim of this article is to give summary about essential things in diagnosing and treating heart failure patients. 3D (accurate diagnoses, evidence based drugs, and new devices are the most important but what to do and what not to do in dealing with heart failure is also useful for your daily practice. (Med J Indones 2012;21:52-8Keywords: Devices, diagnostic, drugs, heart failure

  20. Metabolism of the benzidine-based azo dye Direct Black 38 by human intestinal microbiota

    Energy Technology Data Exchange (ETDEWEB)

    Manning, B.W.; Cerniglia, C.E.; Federle, T.W.

    1985-07-01

    Benzidine-based azo dyes are proven mutagens and have been linked to bladder cancer. Previous studies have indicated that their initial reduction is the result of the azo reductase activity of the intestinal microbiota. Metabolism of the benzidine-based dye Direct Black 38 was examined by using a semicontinuous culture system that simulates the lumen of the human large intestine. The system was inoculated with freshly voided feces, and an active flora was maintained as evidenced by volatile fatty acid and gas production. Within 7 days after exposure to the dye, the following metabolites were isolated and identified by gas chromatography - mass spectrometry: benzidine, 4-aminobiphenyl, monoacetylbenzidine, and acetylaminobiphenyl. Benzidine reached its peak level after 24 h, accounting for 39.1% of the added dye. Its level began to decline, and by day 7 the predominant metabolite was acetylaminobiphenyl, which accounted for 51.1% of the parent compound. Formation of the deaminated and N-acetylated analogs of benzidine, which have enhanced mutagenicity and lipophilicity, previously has not been attributed to the intestinal microbiota.

  1. Novel edible coating based on aloe vera gel to maintain table grape quality and safety.

    Science.gov (United States)

    Valverde, Juan Miguel; Valero, Daniel; Martínez-Romero, Domingo; Guillén, Fabián; Castillo, Salvador; Serrano, María

    2005-10-01

    A novel edible coating based on Aloe vera gel obtained according to SP Patent Filed 200302937 has been used as a means of preservation to maintain the quality and safety of cv. Crimson Seedless table grapes during cold storage and subsequent shelf life. Table grapes have a crucial economic value as a dessert fruit, but once harvested show a reduction of shelf life due to a rapid loss of quality. Uncoated clusters showed a rapid deterioration with an estimated shelf life period of 7 days at 1 degrees C plus 4 days at 20 degrees C, based on the fast weight loss, color changes, accelerated softening and ripening, rachis browning, and high incidence of berry decay. On the contrary, those clusters treated with A. vera gel significantly delayed the above parameters related to postharvest quality losses, and storability could be extended up to 35 days at 1 degrees C. Interestingly, this edible coating was able to reduce the initial microbial counts for both mesophillic aerobic and yeast and molds, which significantly increased in uncoated berries over storage. Moreover, the sensory analyses revealed beneficial effects in terms of delaying rachis browning and dehydration and maintenance of the visual aspect of the berry without any detrimental effect on taste, aroma, or flavors. To the authors' knowledge, this is the first time A. vera gel has been used as an edible coating in fruits, which would be an innovative and interesting means for commercial application and an alternative to the use of postharvest chemical treatments. PMID:16190634

  2. Conversion of borate-based glass scaffold to hydroxyapatite in a dilute phosphate solution.

    Science.gov (United States)

    Liu, Xin; Pan, Haobo; Fu, Hailuo; Fu, Qiang; Rahaman, Mohamed N; Huang, Wenhai

    2010-02-01

    Porous scaffolds of a borate-based glass (composition in mol%: 6Na2O, 8K2O, 8MgO, 22CaO, 36B2O3, 18SiO2, 2P2O5), with interconnected porosity of approximately 70% and pores of size 200-500 microm, were prepared by a polymer foam replication technique. The degradation of the scaffolds and conversion to a hydroxyapatite-type material in a 0.02 M K2HPO4 solution (starting pH = 7.0) at 37 degrees C were studied by measuring the weight loss of the scaffolds, as well as the pH and the boron concentration of the solution. X-ray diffraction, scanning electronic microscopy and energy dispersive x-ray analysis showed that a hydroxyapatite-type material was formed on the glass surface within 7 days of immersion in the phosphate solution. Cellular response to the scaffolds was assessed using murine MLO-A5 cells, an osteogenic cell line. Scanning electron microscopy showed that the scaffolds supported cell attachment and proliferation during the 6 day incubation. The results indicate that this borate-based glass could provide a promising degradable scaffold material for bone tissue engineering applications. PMID:20057014

  3. Conversion of borate-based glass scaffold to hydroxyapatite in a dilute phosphate solution

    Energy Technology Data Exchange (ETDEWEB)

    Liu Xin; Huang Wenhai [Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai 200092 (China); Pan Haobo [Department of Orthopaedics and Traumatology, University of Hong Kong, 999077 (Hong Kong); Fu Hailuo; Fu Qiang; Rahaman, Mohamed N, E-mail: tsliuxin@hotmail.co, E-mail: whhuang@tongji.edu.c [Department of Materials Science and Engineering, and Center for Bone and Tissue Repair and Regeneration, Missouri University of Science and Technology, MO 65409 (United States)

    2010-02-15

    Porous scaffolds of a borate-based glass (composition in mol%: 6Na{sub 2}O, 8K{sub 2}O, 8MgO, 22CaO, 36B{sub 2}O{sub 3}, 18SiO{sub 2}, 2P{sub 2}O{sub 5}), with interconnected porosity of {approx}70% and pores of size 200-500{mu}m, were prepared by a polymer foam replication technique. The degradation of the scaffolds and conversion to a hydroxyapatite-type material in a 0.02 M K{sub 2}HPO{sub 4} solution (starting pH = 7.0) at 37 deg. C were studied by measuring the weight loss of the scaffolds, as well as the pH and the boron concentration of the solution. X-ray diffraction, scanning electronic microscopy and energy dispersive x-ray analysis showed that a hydroxyapatite-type material was formed on the glass surface within 7 days of immersion in the phosphate solution. Cellular response to the scaffolds was assessed using murine MLO-A5 cells, an osteogenic cell line. Scanning electron microscopy showed that the scaffolds supported cell attachment and proliferation during the 6 day incubation. The results indicate that this borate-based glass could provide a promising degradable scaffold material for bone tissue engineering applications.

  4. Law enforcers recognition level emerging threats based on physical appearance and behavior signs the enemy

    Directory of Open Access Journals (Sweden)

    Radzievskiy R.M.

    2015-02-01

    Full Text Available Purpose: examine the effectiveness of the training method of differential approach to the choice of means of influence on the action of law enforcers opponent with different levels of aggressiveness. Material : the experiment involved 15 students of the Kyiv National Academy of Internal Affairs and the 15 employees of the State Guard of Ukraine. Results : presented curriculum for special physical and tactical training. The program details the conceptual apparatus of THREATS and DANGERS manifestations of different levels of aggressiveness opponent (case analysis of its motor behavior. The study participants underwent 7 day course focused training. The basis of the course is an advanced theoretical base. The base is aimed at developing knowledge and skills of employees in determining the level of danger. Including threats from testing and modeling episodes of extreme situations the options cadets. Conclusions : In the simulated collision situations with aggressive opponent to the students significantly improved the adequacy of the response to the threat of execution time and within the legal grounds. Recognition was determined by the level of aggressiveness manifest manners enemy, his emotions, motivation, motor behavior, positional arrangement for 2 - 3 seconds. The program contributed to the development of qualities: attention, orientation, perception, motor lead.

  5. Staining susceptibility of methacrylate and silorane-based materials: influence of resin type and storage time

    Directory of Open Access Journals (Sweden)

    Leonardo Fernandes da Cunha

    2013-06-01

    Full Text Available Introduction: The color stability of composite resins is a fundamental factor in their clinical behavior. Objective: To evaluate the color stability of composite resins of different colors exposed to a cola-based soft drink after different storage periods. Additionally, three methacrylate-based materials and one silorane-based material were evaluated. Material and methods: Specimens of three methacrylate-based materials (Opallis EA3, DA3 and T-Neutral; Filtek Supreme XT A3E, A3D and CT; 4 Seasons A3 Enamel, A3 Dentin and High Value and one silorane-based material (Filtek P90 A3 were prepared, light-cured for 40 s, and manually polished with Sof-Lex discs. Samples were stored for 1 h, 24 h or 7 days. The color was evaluated by CIE-Lab system before and after immersion for 10 min in a cola-based soft drink. Color variation (∆E was calculated from individual values of L*, a* and b*, being considered imperceptible when < 1, clinically acceptable when ≤ 3.3, and clinically inacceptable when higher than 3.3. Data were evaluated by two-way Anova and Dunnett’s T3 tests (α = 0.05. Results: There were differences among the resins (p < 0.001, with an interaction effect being also observed (p < 0.001. Storage time was not significant (p = 0.246. P90 showed a ∆E smaller than one unit at all studied times. Supreme XT CT and 4 Seasons High Value showed higher ∆E, but not above the critical value of 3.3. The only material that showed ∆E higher than 3.3 was Opallis DA3 after 1 h of storage. Conclusion: The silorane-based composite resin showed smaller ∆E at the times studied.

  6. PREVALENCE OF ADVERSE PREGNANCY OUTCOMES: A COMMUNITY BASED LONGITUDINAL STUDY

    Directory of Open Access Journals (Sweden)

    Vidya

    2015-06-01

    Full Text Available BACKGROUND: In most developed countries, pregnancies are planned, complications are few and outcomes are generally favorable for both mother and infant. But in developing countries, adverse pregnancy outcomes are far more frequent due to various reasons. T he most severe adverse outcome of pregnancy is the death of the mother or her offspring. Over the years maternal and child health programmes are striving to improve the health status of pregnant women and neonates. However, the adverse pregnancy outcomes ( M aternal and N eonatal still remain high. OBJECTIVE: To study the prevalence of adverse pregnancy in the study area. METHODOLOGY: A community based longitudinal study was carried out in the 36 villages of Kaiwara from January 2011 to December 2011. All the antenatal mothers were traced through Anganwadi records maintained at different villages. They were contacted at their residence and the questionnaire was administered in their local language. The questionnaire was administered during three different visi ts to collect information regarding socio - demographic details, pregnancy outcomes. The first visit was made before delivery and subsequently second and third visits were made within 7 days and 42 nd day after delivery respectively. Maternal and child protec tion cards were used to validate the collected information. Statistical analysis was performed using SPSS software version 18.0 RESULTS: The present study revealed that, the proportion of low birth weight in the study area was 31.9% (95% CI=25.74 - 38.06, p reterm birth 20.5% (95% CI=15.28 - 25.72, postnatal complications 5% (95% CI=14.819 - 9.181, abortion 2.1% (95% CI=0.25 - 3.95, maternal death 0.4% (95% CI=0.416 - 1.216 and neonatal death 0.4% (95% CI=0.416 - 1.216. CONCLUSION: The present study revealed that the proportion of adverse pregnancy outcomes was in par with the national average.

  7. Toxicodynamics of rigid polystyrene microparticles on pulmonary gas exchange in mice: Implications for microemboli-based drug delivery systems

    Energy Technology Data Exchange (ETDEWEB)

    Kutscher, H.L. [Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 (United States); Gao, D.; Li, S. [Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 (United States); UMDNJ-Rutgers CounterACT Research Center of Excellence, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 (United States); Massa, C.B.; Cervelli, J. [Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 (United States); Deshmukh, M. [Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 (United States); UMDNJ-Rutgers CounterACT Research Center of Excellence, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 (United States); Joseph, L.B.; Laskin, D.L. [UMDNJ-Rutgers CounterACT Research Center of Excellence, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 (United States); Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 (United States); Sinko, P.J., E-mail: sinko@rci.rutgers.edu [Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 (United States); UMDNJ-Rutgers CounterACT Research Center of Excellence, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 (United States)

    2013-01-15

    The toxicodynamic relationship between the number and size of pulmonary microemboli resulting from uniformly sized, rigid polystyrene microparticles (MPs) administered intravenously and their potential effects on pulmonary gas exchange were investigated. CD-1 male mice (6–8 weeks) were intravenously administered 10, 25 and 45 μm diameter MPs. Oxygen hemoglobin saturation in the blood (SpO{sub 2}) was measured non-invasively using a pulse oximeter while varying inhaled oxygen concentration (F{sub I}O{sub 2}). The resulting data were fit to a physiologically based non-linear mathematical model that estimates 2 parameters: ventilation–perfusion ratio (V{sub A}/Q) and shunt (percentage of deoxygenated blood returning to systemic circulation). The number of MPs administered prior to a statistically significant reduction in normalized V{sub A}/Q was dependent on particle size. MP doses that resulted in a significant reduction in normalized V{sub A}/Q one day post-treatment were 4000, 40,000 and 550,000 MPs/g for 45, 25 and 10 μm MPs, respectively. The model estimated V{sub A}/Q and shunt returned to baseline levels 7 days post-treatment. Measuring SpO{sub 2} alone was not sufficient to observe changes in gas exchange; however, when combined with model-derived V{sub A}/Q and shunt early reversible toxicity from pulmonary microemboli was detected suggesting that the model and physical measurements are both required for assessing toxicity. Moreover, it appears that the MP load required to alter gas exchange in a mouse prior to lethality is significantly higher than the anticipated required MP dose for effective drug delivery. Overall, the current results indicate that the microemboli-based approach for targeted pulmonary drug delivery is potentially safe and should be further explored. -- Highlights: ► Murine pulmonary gas exchange after microembolization was non-invasively studied. ► A physiologically based model quantified impairment of pulmonary gas exchange.

  8. Toxicodynamics of rigid polystyrene microparticles on pulmonary gas exchange in mice: Implications for microemboli-based drug delivery systems

    International Nuclear Information System (INIS)

    The toxicodynamic relationship between the number and size of pulmonary microemboli resulting from uniformly sized, rigid polystyrene microparticles (MPs) administered intravenously and their potential effects on pulmonary gas exchange were investigated. CD-1 male mice (6–8 weeks) were intravenously administered 10, 25 and 45 μm diameter MPs. Oxygen hemoglobin saturation in the blood (SpO2) was measured non-invasively using a pulse oximeter while varying inhaled oxygen concentration (FIO2). The resulting data were fit to a physiologically based non-linear mathematical model that estimates 2 parameters: ventilation–perfusion ratio (VA/Q) and shunt (percentage of deoxygenated blood returning to systemic circulation). The number of MPs administered prior to a statistically significant reduction in normalized VA/Q was dependent on particle size. MP doses that resulted in a significant reduction in normalized VA/Q one day post-treatment were 4000, 40,000 and 550,000 MPs/g for 45, 25 and 10 μm MPs, respectively. The model estimated VA/Q and shunt returned to baseline levels 7 days post-treatment. Measuring SpO2 alone was not sufficient to observe changes in gas exchange; however, when combined with model-derived VA/Q and shunt early reversible toxicity from pulmonary microemboli was detected suggesting that the model and physical measurements are both required for assessing toxicity. Moreover, it appears that the MP load required to alter gas exchange in a mouse prior to lethality is significantly higher than the anticipated required MP dose for effective drug delivery. Overall, the current results indicate that the microemboli-based approach for targeted pulmonary drug delivery is potentially safe and should be further explored. -- Highlights: ► Murine pulmonary gas exchange after microembolization was non-invasively studied. ► A physiologically based model quantified impairment of pulmonary gas exchange. ► Number and size of microemboli determine severity

  9. Preparation, characterization and biological test of 3D-scaffolds based on chitosan, fibroin and hydroxyapatite for bone tissue engineering

    Energy Technology Data Exchange (ETDEWEB)

    Lima, Paulo Autran Leite; Resende, Cristiane Xavier [Departamento de Ciências de Materiais, Universidade Federal de Sergipe, Av. Marechal Rondon, s/n. Jardim Rosa Elze, São Cristóvão, Sergipe CEP 49000-100 (Brazil); Dulce de Almeida Soares, Glória [Departamento de Ciências de Materiais, Universidade Federal do Rio de Janeiro, Av. Brigadeiro Trompowisk, s/n. Ilha do Fundão, Rio de Janeiro, Rio de Janeiro CEP 21900-000 (Brazil); Anselme, Karine [Institut de Science des Matériaux de Mulhouse (IS2M), CNRS LRC7228, 15, Jean Starcky Street, BP 2488, 68054 Mulhouse cedex (France); Almeida, Luís Eduardo, E-mail: lealmeida2009@gmail.com [Departamento de Ciências de Materiais, Universidade Federal de Sergipe, Av. Marechal Rondon, s/n. Jardim Rosa Elze, São Cristóvão, Sergipe CEP 49000-100 (Brazil)

    2013-08-01

    This work describes the preparation and characterization of porous 3D-scaffolds based on chitosan (CHI), chitosan/silk fibroin (CHI/SF) and chitosan/silk fibroin/hydroxyapatite (CHI/SF/HA) by freeze drying. The biomaterials were characterized by X-ray diffraction, attenuated total reflection Fourier transform infrared spectroscopy, thermogravimetric analysis, differential scanning calorimetry, scanning electron microscopy and energy dispersive spectroscopy. In addition, studies of porosity, pore size, contact angle and biological response of SaOs-2osteoblastic cells were performed. The CHI scaffolds have a porosity of 94.2 ± 0.9%, which is statistically higher than the one presented by CHI/SF/HA scaffolds, 89.7 ± 2.6%. Although all scaffolds were able to promote adhesion, growth and maintenance of osteogenic differentiation of SaOs-2 cells, the new 3D-scaffold based on CHI/SF/HA showed a significantly higher cell growth at 7 days and 21 days and the level of alkaline phosphatase at 14 and 21 days was statistically superior compared to other tested materials. - Highlights: • Preparation of 3D-scaffolds based on CHI, with or without addition of SF and HA. • Scaffolds exhibited interconnected porous structure (pore size superior to 50 μm). • The tripolyphosphate did not induce any significant cytotoxic response. • The CHI/SF/HA composite showed a higher cell growth and ALP activity.

  10. Preparation, characterization and biological test of 3D-scaffolds based on chitosan, fibroin and hydroxyapatite for bone tissue engineering

    International Nuclear Information System (INIS)

    This work describes the preparation and characterization of porous 3D-scaffolds based on chitosan (CHI), chitosan/silk fibroin (CHI/SF) and chitosan/silk fibroin/hydroxyapatite (CHI/SF/HA) by freeze drying. The biomaterials were characterized by X-ray diffraction, attenuated total reflection Fourier transform infrared spectroscopy, thermogravimetric analysis, differential scanning calorimetry, scanning electron microscopy and energy dispersive spectroscopy. In addition, studies of porosity, pore size, contact angle and biological response of SaOs-2osteoblastic cells were performed. The CHI scaffolds have a porosity of 94.2 ± 0.9%, which is statistically higher than the one presented by CHI/SF/HA scaffolds, 89.7 ± 2.6%. Although all scaffolds were able to promote adhesion, growth and maintenance of osteogenic differentiation of SaOs-2 cells, the new 3D-scaffold based on CHI/SF/HA showed a significantly higher cell growth at 7 days and 21 days and the level of alkaline phosphatase at 14 and 21 days was statistically superior compared to other tested materials. - Highlights: • Preparation of 3D-scaffolds based on CHI, with or without addition of SF and HA. • Scaffolds exhibited interconnected porous structure (pore size superior to 50 μm). • The tripolyphosphate did not induce any significant cytotoxic response. • The CHI/SF/HA composite showed a higher cell growth and ALP activity

  11. Self-healing of Early Age Cracks in Cement-based Materials by Mineralization of Carbonic Anhydrase Microorganism

    Directory of Open Access Journals (Sweden)

    Chunxiang eQian

    2015-11-01

    Full Text Available This research investigated the self-healing potential of early age cracks in cement-based materials incorporating the bacteria which can produce carbonic anhydrase. Cement-based materials specimens were pre-cracked at the age of 7, 14, 28, 60 days to study the repair ability influenced by cracking time, the width of cracks were between 0.1 and 1.0 mm to study the healing rate influenced by width of cracks. The experimental results indicated that the bacteria showed excellent repairing ability to small cracks formed at early age of 7 days, cracks below 0.4 mm was almost completely closed. The repair effect reduced with the increasing of cracking age. Cracks width influenced self-healing effectiveness significantly. The transportation of CO2 and Ca2+ controlled the self-healing process. The computer simulation analyses revealed the self-healing process and mechanism of microbiologically precipitation induced by bacteria and the depth of precipitated CaCO3 could be predicted base on valid Ca2+.

  12. Surface Roughness, Microhardness, and Microleakage of a Silorane-Based Composite Resin after Immediate or Delayed Finishing/Polishing.

    Science.gov (United States)

    Lins, Fernanda Carvalho Rezende; Ferreira, Raquel Conceição; Silveira, Rodrigo Richard; Pereira, Carolina Nemésio Barros; Moreira, Allyson Nogueira; Magalhães, Claudia Silami

    2016-01-01

    Objective. This study evaluated the effect of immediate or delayed finishing/polishing using different systems on the surface roughness, hardness, and microleakage of a silorane-based composite. Material and Methods. Specimens were made with silorane-based composite (Filtek P90, 3M ESPE) and assigned to the treatments: control (light-cured); aluminum oxide discs (Sof-Lex, 3M ESPE); diamond-impregnated silicone tips (Astropol, Ivoclar Vivadent); aluminum oxide-impregnated silicone tips (Enhance, Dentsply). Half of the specimens were finished/polished immediately and the rest after 7 days. Surface roughness (Ra, μm; n = 20) and Vickers microhardness (50 g; 45 s; n = 10) were measured. Cavities were prepared in bovine incisors and filled with Filtek P90. The fillings received immediate or delayed finishing/polishing (n = 10) and were subjected to dye penetration test (0.5% basic fuchsin, 24 h). Data were analyzed by ANOVA and Scheffe, Kruskal-Wallis, and Mann-Whitney tests (p < 0.05). Results. The finishing/polishing system significantly influenced roughness and microhardness (p < 0.0001). For enamel, microleakage was not affected by the finishing/polishing system (p = 0.309). For dentin, Sof-Lex discs and Astropol points promoted greater microleakage than Enhance points (p = 0.033). Conclusion. Considering roughness, microhardness, and microleakage together, immediate finishing/polishing of a silorane-based composite using aluminum oxide discs may be recommended. PMID:26977150

  13. Touch BASE

    CERN Multimedia

    Antonella Del Rosso

    2015-01-01

    In a recent Nature article (see here), the BASE collaboration reported the most precise comparison of the charge-to-mass ratio of the proton to its antimatter equivalent, the antiproton. This result is just the beginning and many more challenges lie ahead.   CERN's AD Hall, where the BASE experiment is set-up. The Baryon Antibaryon Symmetry Experiment (BASE) was approved in June 2013 and was ready to take data in August 2014. During these 14 months, the BASE collaboration worked hard to set up its four cryogenic Penning traps, which are the heart of the whole experiment. As their name indicates, these magnetic devices are used to trap antiparticles – antiprotons coming from the Antiproton Decelerator – and particles of matter – negative hydrogen ions produced in the system by interaction with a degrader that slows the antiprotons down, allowing scientists to perform their measurements. “We had very little time to set up the wh...

  14. Comparative in-vitro efficacy of fluoroquinolones against Streptococcus pneumoniae recovered from bacterial keratitis as determined by E-test

    Directory of Open Access Journals (Sweden)

    Ramakrishnan R

    2010-04-01

    Full Text Available Background and Objectives: The advent of new fluoroquinolones has drawn the attention for reliable methods on the in-vitro susceptibility testing of Streptococccus pneumoniae. This study attempts to determine the minimum inhibitory concentration (MIC of second-generation (ciprofloxacin and ofloxacin, third-generation (levofloxacin and the fourth-generation (moxifloxacin and gatifloxacin fluoroquinolones against S. pneumoniae recovered from bacterial keratitis. Materials and Methods: In retrospect, the MICs of 50 strains of S. pneumoniae isolated from the corneal scrapes of patients with bacterial keratitis were determined against ciprofloxacin, ofloxacin, levofloxacin, gatifloxacin and moxifloxacin using E-tests. The National Committee of Clinical Laboratory Standards (NCCLS susceptibility patterns and the potencies of the MICs were statistically compared. Results: The median MIC of ciprofloxacin (0.25μg/ml was found to be lower than the median MICs of ofloxacin (0.5μg/ml (P < 0.449 and levofloxacin (1.0μg/ml (P < 0.001. The median MICs of gatifloxacin (0.1μg/ml was lower than the median MICs of ciprofloxacin (0.25μg/ml (P < 0.001, ofloxacin (0.5μg/ml (P < 0.001 and levofloxacin (1.0μg/ml (P < 0.001. Moxifloxacin (0.06μg/ml had showed lower median MICs than gatifloxacin (0.1μg/ml (P < 0.001 levofloxacin (1.0μg/ml (P < 0.001, ofloxacin (0.5μg/ml (P < 0.001 and ciprofloxacin (0.25μg/ml (P < 0.001. Moxifloxacin (0.06μg/ml had a lower MIC50 (μg/ml than gatifloxacin (0.1μg/ml, levofloxacin (1.0μg/ml, ciprofloxacin (0.25μg/ml and ofloxacin (0.5μg/ml. MIC90 (μg/ml of moxifloxacin (0.06μg/ml was found to be lower than the MIC90 (μg/ml of gatifloxacin (0.5μg/ml, levofloxacin (1.0μg/ml, ofloxacin (0.5μg/ml and ciprofloxacin (0.5μg/ml. Conclusion: Based on in-vitro testing, the five portrayed fluoroquinolones 100% sensitivity to S. pneumoniae. However, the fourth-generation fluoroquinolone, moxifloxacin appeared to be more

  15. A protein-based hydrogel for in vitro expansion of mesenchymal stem cells.

    Directory of Open Access Journals (Sweden)

    Jingyu Wang

    Full Text Available Hydrogels are widely used as scaffolds in tissue engineering because they can provide excellent environments for bioactive components including growth factors and cells. We reported in this study on a physical hydrogel formed by a specific protein-peptide interaction, which could be used for the three dimensional (3D cell culture of murine mesenchymal stem cells (mMSC. The mMSC kept dividing during the 7-day culture period and the metabolic-active cell number at day 7 was 359% more than that at day 1. This kind of physical hydrogel could be converted to a homogeneous solution by firstly adding an equal volume of culture medium and then pipeting for several times. Therefore, mMSC post culture could be easily separated from cell-gel constructs. We believed that the protein-based hydrogel system in this study could be developed into a promising scaffold for in vitro expansion of stem cells and cell therapy. This work would be in the general interests of researchers in the fields of biomaterials and supramolecular chemistry.

  16. The fate of free radicals in a cellulose based hydrogel: detection by electron paramagnetic resonance spectroscopy.

    Science.gov (United States)

    Basumallick, Lipika; Ji, J Andrea; Naber, Nariman; Wang, Y John

    2009-07-01

    Cellulose derivatives are commonly used as gelling agents in topical and ophthalmic drug formulations. During the course of manufacturing, cellulose derivatives are believed to generate free radicals. These free radicals may degrade the gelling agent, leading to lower viscosity. Free radicals also may react with the active ingredient in the product. The formation of radicals in a 3% hydrogel of hypromellose (hydroxypropyl methylcellulose) was monitored by electron paramagnetic resonance (EPR) spectroscopy and spin trapping techniques. Radicals were trapped with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) and quantitated by comparing the EPR intensity with 4-hydroxy-2,2,6,6-tetramethylpiperidinyloxy (TEMPOL), a stable free radical. Typically, the hydrogels showed an initial increase in the radical concentration within 2 days after autoclaving, followed by a drop in radical concentration in 7 days. EDTA prevented the formation of free radicals in the hypromellose (HPMC) hydrogel, suggesting the involvement of metal ions in the generation of free radicals. The oxidizing potential of the hydrogel was estimated by measuring the rate at which methionine (a model for the protein active pharmaceutical ingredient) was degraded, and was consistent with the amount of radicals present in the gel. This study is the first report investigating the application of EPR spectroscopy in detecting and estimating free radical concentration in cellulose based hydrogels. PMID:19090570

  17. Evaluation Clinical Effects of Anti-Septic Solution Based on Propolis in Caries-Active Children

    Directory of Open Access Journals (Sweden)

    Rossana Vanessa Dantas de ALMEIDA

    2006-04-01

    Full Text Available Objective: This study aimed validate the action of one anti-septic solution based on propolis against the clinical indexes and levels of S. mutans. Method: The antimicrobial activity of the extract was done on solid plates to determinate the Minimum Inhibitory Concentration (MIC using sorts of S. mutans. With the MIC of the extract, was made a mouthrinse solution of propolis (6.25%, which was used clinically as a test solution and compared with positive control solution, the chlorexidine (0.12%. Through the crossover design, fifteen children used the mouthrinse solution of propolis during 15 consecutive days and after a break of 21 days, they used daily mouthrinse with the chlorexidine. Were collected indexes for accumulation of oral biofilm (OHI-S and for gun disease (GBI before (T0 and 24 hours after (T1 the use of the solutions, besides of the couting of the S. mutans from saliva's samples before (T0 and 24 hours (T0, 7 days (T7, 15 days (T15 e 21 days (T21 after the end of both mouthrinses. Results: The results demonstrated significative reduction of the S. mutans levels 24 hours (p0.05. Conclusion: The propolis extract showed satisfactory antimicrobial activity and close to the chlorexidine's action, besides of act against clinical conditions of oral biofilm's presence and gun disease what becomes it useful as therapeutic agent.

  18. Physiologically Based Pharmacokinetic Modeling Framework for Quantitative Prediction of an Herb–Drug Interaction

    Science.gov (United States)

    Brantley, S J; Gufford, B T; Dua, R; Fediuk, D J; Graf, T N; Scarlett, Y V; Frederick, K S; Fisher, M B; Oberlies, N H; Paine, M F

    2014-01-01

    Herb–drug interaction predictions remain challenging. Physiologically based pharmacokinetic (PBPK) modeling was used to improve prediction accuracy of potential herb–drug interactions using the semipurified milk thistle preparation, silibinin, as an exemplar herbal product. Interactions between silibinin constituents and the probe substrates warfarin (CYP2C9) and midazolam (CYP3A) were simulated. A low silibinin dose (160 mg/day × 14 days) was predicted to increase midazolam area under the curve (AUC) by 1%, which was corroborated with external data; a higher dose (1,650 mg/day × 7 days) was predicted to increase midazolam and (S)-warfarin AUC by 5% and 4%, respectively. A proof-of-concept clinical study confirmed minimal interaction between high-dose silibinin and both midazolam and (S)-warfarin (9 and 13% increase in AUC, respectively). Unexpectedly, (R)-warfarin AUC decreased (by 15%), but this is unlikely to be clinically important. Application of this PBPK modeling framework to other herb–drug interactions could facilitate development of guidelines for quantitative prediction of clinically relevant interactions. PMID:24670388

  19. In situ gelling polyvalerolactone-based thermosensitive hydrogel for sustained drug delivery.

    Science.gov (United States)

    Mishra, Gyan P; Kinser, Reid; Wierzbicki, Igor H; Alany, Raid G; Alani, Adam W G

    2014-10-01

    Biodegradable poly(ethyleneglycol)-poly(valerolactone)-poly(ethyleneglycol) [PEG-PVL-PEG] copolymers were synthesized through ring opening polymerization of δ-valerolactone (VL) followed by the coupling of monomethoxy poly(ethyleneglycol-poly(valerolactone) (mPEG-PVL) with hexamethylene diisocyanate (HDI). The copolymers were characterized by (1)H NMR, FT-IR, and GPC. Block copolymers of PEG and PVL with different VL/PEG molar ratios were successfully synthesized. One of the copolymers (Copolymer 2, PEG550-PVL6768-PEG550) displayed a sol-gel transition at a physiological temperature based on the test tube inverting method and rheological studies. The thermogelling copolymer demonstrated a characteristic crystalline peak for PVL block as determined by DSC and XRD analysis. In vitro release from the copolymer hydrogel matrix indicated that dexamethasone (DEX), a hydrophobic model drug, released comparatively slower than 5-fluoruracil (5-FU), a hydrophilic model drug, due to the potential partitioning of DEX into the PVL core. 5-FU in vitro release from copolymer 2 was 86% in 22 h, whereas only 14% of DEX was released in 24h. Cell viability studies confirmed that hydrogels composed of block copolymers are biocompatible. Copolymer 2 showed more than 80% relative cell viability at all concentrations, including concentrations greater than 200 fold CMC. In vivo gel formation studies indicate that gel integrity was maintained for 7 days upon subcutaneous injection into mice. These results indicate that PEG-PVL-PEG copolymers are suitable for drug delivery applications. PMID:24931340

  20. Preliminary optimization analysis of the radiation shielding of the China Lead-based Research Reactor

    International Nuclear Information System (INIS)

    Accelerator Driven subcritical System (ADS) is recognized as an efficient nuclear waste transmutation device. Supported by the Strategic Priority Research Program of 'the Future Advanced Nuclear Fission Energy-ADS transmutation system', the China LEAd-based Research Reactor (CLEAR-I) is proposed. Along with the approaching of the CLEAR-I design, the radiation shielding for CLEAR-I is updated and optimized step by step to meet with new shielding requirements. Employing the modeling program MCAM and calculation system VisualBUS developed by FDS Team, the shielding capability was verified using Monte Carlo method. As shown from the results, the fast neutron flux for components in reactor vessel is under the limitation and the neutron radiation for mechanism in containing room has been as low as possible. After shutdown for 7 days, the dose rate in most area of containing room is lower than 100 μSv/hr, allowing hands on operation. Replacement of components such as the spallation target in containing room is possible. (author)

  1. Benefits of Sealed-Curing on Compressive Strength of Fly Ash-Based Geopolymers

    Directory of Open Access Journals (Sweden)

    Sujeong Lee

    2016-07-01

    Full Text Available There is no standardized procedure for producing geopolymers; therefore, many researchers develop their own procedures for mixing and curing to achieve good workability and strength development. The curing scheme adopted is important in achieving maximum performance of resultant geopolymers. In this study, we evaluated the impact of sealed and unsealed curing on mechanical strength of geopolymers. Fly ash-based geopolymers cured in sealed and unsealed moulds clearly revealed that retention of water during curing resulted in superior strength development. The average compressive strength of sealed-cured geopolymers measured after 1 day of curing was a modest 50 MPa, while after 7 day curing the average compressive strength increased to 120~135 MPa. In the unsealed specimens the average compressive strength of geopolymers was lower; ranging from 60 to 90 MPa with a slight increase as the curing period increased. Microcracking caused by dehydration is postulated to cause the strength decrease in the unsealed cured samples. These results show that water is a crucial component for the evolution of high strength three-dimensional cross-linked networks in geopolymers.

  2. Peak load forecasting using Bayesian regularization, Resilient and adaptive backpropagation learning based artificial neural networks

    Energy Technology Data Exchange (ETDEWEB)

    Saini, Lalit Mohan [Department of Electrical Engineering, National Institute of Technology, Kurukshetra, Haryana 136119 (India)

    2008-07-15

    Up to 7 days ahead electrical peak load forecasting has been done using feed forward neural network based on Steepest descent, Bayesian regularization, Resilient and adaptive backpropagation learning methods, by incorporating the effect of eleven weather parameters and past peak load information. To avoid trapping of network into a state of local minima, the optimization of user-defined parameters viz., learning rate and error goal has been performed. The sliding window concept has been incorporated for selection of training data set. It was then reduced as per relevant selection according to the day type and season for which the forecast is made. To reduce the dimensionality of input matrix, the Principal Component Analysis method of factor extraction or correlation analysis technique has been used and their performance has been compared. The resultant data set was used for training of three-layered neural network. In order to increase the learning speed, the weights and biases were initialized according to Nguyen and Widrow method. To avoid over fitting, early stopping of training was done at the minimum validation error. (author)

  3. Development of a ground-based space micro-algae photo-bioreactor

    Science.gov (United States)

    Ai, W.; Guo, S.; Qin, L.; Tang, Y.

    The purpose of the research is to develop a photo-bioreactor which may produce algae protein and oxygen for future astronauts in comparatively long-term exploration, and remove carbon dioxide in a controlled ecological life support system. Based on technical parameters and performance requirements, the project planning, design drafting, and manufacture were conducted. Finally, a demonstration test for producing algae was done. Its productivity for micro-algae and performance of the photo-bioreactor were evaluated. The facility has nine subsystems, including the reactor, the illuminating unit, the carbon dioxide (CO2) production unit and oxygen (O2) generation unit, etc. The demonstration results showed that the facility worked well, and the parameters, such as energy consumption, volume, and productivity for algae, met with the design requirement. The density of algae in the photo-bioreactor increased from 0.174 g (dry weight) L-1 to 4.064 g (dry weight) L-1 after 7 days growth. The principle of providing CO2 in the photo-bioreactor for algae and removing O2 from the culture medium was suitable for the demand of space conditions. The facility has reasonable technical indices, and smooth and dependable performances.

  4. Web based foundry knowledge base

    Directory of Open Access Journals (Sweden)

    A. Stawowy

    2009-01-01

    Full Text Available The main assumptions and functions of proposed Foundry Knowledge Base (FKB are presented in this paper. FKB is a framework forinformation exchange of casting products and manufacturing methods. We use CMS (Content Management System to develope andmaintain our web-based system. The CastML – XML dialect developed by authors for description of casting products and processes – isused as a tool for information interchange between ours and outside systems, while SQL is used to store and edit knowledge rules and alsoto solve the basic selection problems in the rule-based module. Besides the standard functions (companies data, news, events, forums and media kit, our website contains a number of nonstandard functions; the intelligent search module based on expert system is the main advantage of our solution. FKB is to be a social portal which content will be developed by foundry community.

  5. Toxicity of Amphotericin B Deoxycholate-Based Induction Therapy in Patients with HIV-Associated Cryptococcal Meningitis

    Science.gov (United States)

    Bottomley, Christian; Loyse, Angela; Brouwer, Annemarie E.; Muzoora, Conrad; Taseera, Kabanda; Jackson, Arthur; Phulusa, Jacob; Hosseinipour, Mina C.; van der Horst, Charles; Limmathurotsakul, Direk; White, Nicholas J.; Wilson, Douglas; Wood, Robin; Meintjes, Graeme; Harrison, Thomas S.; Jarvis, Joseph N.

    2015-01-01

    Amphotericin B deoxycholate (AmBd) is the recommended induction treatment for HIV-associated cryptococcal meningitis (CM). Its use is hampered by toxicities that include electrolyte abnormalities, nephrotoxicity, and anemia. Protocols to minimize toxicity are applied inconsistently. In a clinical trial cohort of AmBd-based CM induction treatment, a standardized protocol of preemptive hydration and electrolyte supplementation was applied. Changes in blood counts, electrolyte levels, and creatinine levels over 14 days were analyzed in relation to the AmBd dose, treatment duration (short course of 5 to 7 days or standard course of 14 days), addition of flucytosine (5FC), and outcome. In the 368 patients studied, the hemoglobin levels dropped by a mean of 1.5 g/dl (95% confidence interval [CI], 1.0 to 1.9 g/dl) following 7 days of AmBd and by a mean of 2.3 g/dl (95% CI, 1.1 to 3.6 g/dl) after 14 days. Serum creatinine levels increased by 37 μmol/liter (95% CI, 30 to 45 μmol/liter) by day 7 and by 49 μmol/liter (95% CI, 35 to 64μmol/liter) by day 14 of AmBd treatment. Overall, 33% of patients developed grade III/IV anemia, 5.6% developed grade III hypokalemia, 9.5% had creatinine levels that exceeded 220 μmol, and 6% discontinued AmBd prematurely. The addition of 5FC was associated with a slight increase in anemia but not neutropenia. Laboratory abnormalities stabilized or reversed during the second week in patients on short-course induction. Grade III/IV anemia (adjusted odds ratio [aOR], 2.2; 95% CI, 1.1 to 4.3; P = 0.028) and nephrotoxicity (aOR, 4.5; 95% CI, 1.8 to 11; P = 0.001) were risk factors for 10-week mortality. In summary, routine intravenous saline hydration and preemptive electrolyte replacement during AmBd-based induction regimens for HIV-associated CM minimized the incidence of hypokalemia and nephrotoxicity. Anemia remained a concerning adverse effect. The addition of flucytosine was not associated with increased neutropenia. Shorter AmBd courses

  6. In vitro evaluation of borate-based bioactive glass scaffolds prepared by a polymer foam replication method

    International Nuclear Information System (INIS)

    Borate-based bioactive glass scaffolds with a microstructure similar to that of human trabecular bone were prepared using a polymer foam replication method, and evaluated in vitro for potential bone repair applications. The scaffolds (porosity = 72 ± 3%; pore size = 250-500 μm) had a compressive strength of 6.4 ± 1.0 MPa. The bioactivity of the scaffolds was confirmed by the formation of a hydroxyapatite (HA) layer on the surface of the glass within 7 days in 0.02 M K2HPO4 solution at 37 deg. C. The biocompatibility of the scaffolds was assessed from the response of cells to extracts of the dissolution products of the scaffolds, using assays of MTT hydrolysis, cell viability, and alkaline phosphatase activity. For boron concentrations below a threshold value (0.65 mM), extracts of the glass dissolution products supported the proliferation of bone marrow stromal cells, as well as the proliferation and function of murine MLO-A5 cells, an osteogenic cell line. Scanning electron microscopy showed attachment and continuous increase in the density of MLO-A5 cells cultured on the surface of the glass scaffolds. The results indicate that borate-based bioactive glass could be a potential scaffold material for bone tissue engineering provided that the boron released from the glass could be controlled below a threshold value.

  7. Physical activity levels and patterns in older adults: the influence of a DVD-based exercise program.

    Science.gov (United States)

    Gothe, Neha P; Wójcicki, Thomas R; Olson, Erin A; Fanning, Jason; Awick, Elizabeth; Chung, H David; Zuniga, Krystle E; Mackenzie, Michael J; Motl, Robert W; McAuley, Edward

    2015-02-01

    The use of multimedia to influence health behaviors offers unique advantages over more traditional center-based programs, however, little is known about the effectiveness of such approaches in improving physical activity levels over time. The purpose of this study was to examine the efficacy of a progressive and age-appropriate, DVD-delivered exercise program in promoting physical activity levels among older adult cohorts. Community dwelling older adults (N = 307, Mean age = 71 years) were randomized to one of two groups: a 6-month home-based DVD-delivered exercise (i.e., FlexToBa™) intervention group or a healthy aging DVD control group. Physical activity was assessed objectively using a standard 7-day accelerometer wear period and subjectively using the Godin Leisure Time Exercise Questionnaire, at baseline and follow-up. Analysis of covariances indicated a statistically significant treatment effect for subjectively [F(1,250) = 8.42, P = .004, η(2) = .03] and objectively [F(1,240) = 3.77, P = .05, η(2) = .02] measured physical activity. The older cohort (>70) in the FlexToBa condition further had significantly larger improvements in physical activity levels compared to their younger counterparts. From a public health perspective, media-delivered interventions such as the FlexToBa program might prove to be cost-effective, have a broader reach and at the same time be effective in improving physical activity levels in older adults.

  8. The Impact of a Pulmonary-Artery-Catheter-Based Protocol on Fluid and Catecholamine Administration in Early Sepsis

    Directory of Open Access Journals (Sweden)

    Carina Bethlehem

    2012-01-01

    Full Text Available Objective. The pulmonary artery catheter (PAC remains topic of debate. Despite abundant data, it is of note that many trials did not incorporate a treatment protocol. Methods. We retrospectively evaluated fluid balances and catecholamine doses in septic patients after the introduction of a PAC-based treatment protocol in comparison to historic controls. Results. 2×70 patients were included. The first day the PAC group had a significantly higher positive fluid balance in comparison to controls (6.1±2.6 versus 3.8±2.4 litre, <0.001. After 7 days the cumulative fluid balance in the PAC group was significantly lower than in controls (9.4±7.4 versus 13±7.6 litre, =0.001. Maximum dose of norepinephrine was significantly higher in the PAC group. Compared to controls this was associated with a significant reduction in ventilator and ICU days. Conclusions. Introduction of a PAC-based treatment protocol in sepsis changed the administration of fluid and vasopressors significantly.

  9. In vitro evaluation of borate-based bioactive glass scaffolds prepared by a polymer foam replication method

    Energy Technology Data Exchange (ETDEWEB)

    Fu Hailuo, E-mail: fuhailuo@hotmail.com [Department of Materials Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409 (United States); Institute of Bio-Engineering and IT Materials, Tongji University, Shanghai 200092 (China); Fu Qiang, E-mail: fuharry@hotmail.com [Department of Materials Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409 (United States); Zhou Nai [Institute of Bio-Engineering and IT Materials, Tongji University, Shanghai 200092 (China); Huang Wenhai, E-mail: whhuang@mail.tongji.edu.cn [Institute of Bio-Engineering and IT Materials, Tongji University, Shanghai 200092 (China); Rahaman, Mohamed N. [Department of Materials Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409 (United States); Wang Deping [Institute of Bio-Engineering and IT Materials, Tongji University, Shanghai 200092 (China); Liu Xin [Department of Materials Science and Engineering, Missouri University of Science and Technology, Rolla, MO 65409 (United States); Institute of Bio-Engineering and IT Materials, Tongji University, Shanghai 200092 (China)

    2009-08-31

    Borate-based bioactive glass scaffolds with a microstructure similar to that of human trabecular bone were prepared using a polymer foam replication method, and evaluated in vitro for potential bone repair applications. The scaffolds (porosity = 72 {+-} 3%; pore size = 250-500 {mu}m) had a compressive strength of 6.4 {+-} 1.0 MPa. The bioactivity of the scaffolds was confirmed by the formation of a hydroxyapatite (HA) layer on the surface of the glass within 7 days in 0.02 M K{sub 2}HPO{sub 4} solution at 37 deg. C. The biocompatibility of the scaffolds was assessed from the response of cells to extracts of the dissolution products of the scaffolds, using assays of MTT hydrolysis, cell viability, and alkaline phosphatase activity. For boron concentrations below a threshold value (0.65 mM), extracts of the glass dissolution products supported the proliferation of bone marrow stromal cells, as well as the proliferation and function of murine MLO-A5 cells, an osteogenic cell line. Scanning electron microscopy showed attachment and continuous increase in the density of MLO-A5 cells cultured on the surface of the glass scaffolds. The results indicate that borate-based bioactive glass could be a potential scaffold material for bone tissue engineering provided that the boron released from the glass could be controlled below a threshold value.

  10. Rapid detection of listeria spp. using an internalin A aptasensor based on carbon-metal nanohybrid structures

    Science.gov (United States)

    Vanegas, D. C.; Rong, Yue; Schwalb, N.; Hills, K. D.; Gomes, C.; McLamore, E. S.

    2015-05-01

    Foodborne outbreaks caused by Listeria monocytogenes continue to raise major public health concerns worldwide. In the United States alone, the centers for disease control and prevention have confirmed the occurrence of 183 cases of listeriosis with 39 fatalities within the last 3 years. Standard methods for the detection of pathogenic strains require up to 7 days to yield results, thus faster techniques with the same level of reliability for bacteria detection are desirable. This study reports on the development of a rapid, accurate, and sensitive electrochemical biosensor for rapid testing of Listeria spp. based on the selective binding of InlA aptamers to internalins in the cell membrane of the target bacteria. Hybrid nanomaterial platforms based on reduced graphene oxide and nanoplatinum were deposited onto Pt/Ir electrodes for enhancing electrochemical transduction during the recognition events. InlA aptamers were immobilized onto the nanomaterial platforms via metal-thiol adsorption. Aptamer loading onto different platform nanostructures was investigated through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The detection mechanism was evaluated by recording the electrochemical response to several bacterial dilutions in PBS buffer using the non-pathogenic species Listeria innocua. These preliminary results show that the aptasensor can be tuned for detection of Listeria concentrations as low as 100 CFU/ml in less than 3 hours (including incubation time and data analysis). The developed aptasensor opens a promising direction for rapid testing of Listeria monocytogenes in food products.

  11. Systemic Administration of Interleukin 2 Enhances the Therapeutic Efficacy of Dendritic Cell-Based Tumor Vaccines

    Science.gov (United States)

    Shimizu, K.; Fields, R. C.; Giedlin, M.; Mule, J. J.

    1999-03-01

    We have reported previously that murine bone marrow-derived dendritic cells (DC) pulsed with whole tumor lysates can mediate potent antitumor immune responses both in vitro and in vivo. Because successful therapy was dependent on host immune T cells, we have now evaluated whether the systemic administration of the T cell stimulatory/growth promoting cytokine interleukin-2 (IL-2) could enhance tumor lysate-pulsed DC-based immunizations to further promote protective immunity toward, and therapeutic rejection of, syngeneic murine tumors. In three separate approaches using a weakly immunogenic sarcoma (MCA-207), the systemic administration of non-toxic doses of recombinant IL-2 (20,000 and 40,000 IU/dose) was capable of mediating significant increases in the potency of DC-based immunizations. IL-2 could augment the efficacy of tumor lysate-pulsed DC to induce protective immunity to lethal tumor challenge as well as enhance splenic cytotoxic T lymphocyte activity and interferon-γ production in these treated mice. Moreover, treatment with the combination of tumor lysate-pulsed DC and IL-2 could also mediate regressions of established pulmonary 3-day micrometastases and 7-day macrometastases as well as established 14- and 28-day s.c. tumors, leading to either significant cure rates or prolongation in overall survival. Collectively, these findings show that nontoxic doses of recombinant IL-2 can potentiate the antitumor effects of tumor lysate-pulsed DC in vivo and provide preclinical rationale for the use of IL-2 in DC-based vaccine strategies in patients with advanced cancer.

  12. Copula-Based Bivariate Flood Frequency Analysis in a Changing Climate-A Case Study in the Huai River Basin, China

    Institute of Scientific and Technical Information of China (English)

    Kai Duan; Yadong Mei; Liping Zhang

    2016-01-01

    ABSTRACT:Copula-based bivariate frequency analysis can be used to investigate the changes in flood characteristics in the Huai River Basin that could be caused by climate change. The univariate distribu-tions of historical flood peak, maximum 3-day and 7-day volumes in 1961–2000 and future values in 2061–2100 projected from two GCMs (CSIRO-MK3.5 and CCCma-CGCM3.1) under A2, A1B and B1 emission scenarios are analyzed and compared. Then, bivariate distributions of peaks and volumes are constructed based on the copula method and possible changes in joint return periods are characterized. Results indicate that the Clayton copula is more appropriate for historical and CCCma-CGCM3.1 simu-lating flood variables, while that of Frank and Gumbel are better fitted to CSIRO-MK3.5 simulations. The variations of univariate and bivariate return periods reveal that flood characteristics may be more sensi-tive to different GCMs than different emission scenarios. Between the two GCMs, CSIRO-MK3.5 evi-dently predicts much more severe flood conditions in future, especially under B1 scenario, whereas CCCma-CGCM3.1 generally suggests contrary changing signals. This study corroborates that copulas can serve as a viable and flexible tool to connect univariate marginal distributions of flood variables and quantify the associated risks, which may provide useful information for risk-based flood control.

  13. Effectiveness of 0.2% chlorhexidine gel and a eugenol-based paste on postoperative alveolar osteitis in patients having third molars extracted: a randomised controlled clinical trial.

    Science.gov (United States)

    Jesudasan, James Solomon; Wahab, P U Abdul; Sekhar, M R Muthu

    2015-11-01

    The aim of this study was to compare the effect of application of 0.2% chlorhexidine gel, a eugenol-based paste, together with a control group on the postoperative incidence of alveolar osteitis in patients having third molars extracted. A total of 270 patients who had this procedure at the Dept of Oral and Maxillofacial Surgery, Saveetha Dental College and who met the inclusion criteria were enrolled in the study and divided into 3 groups: the first had a 0.2% chlorhexidine-based gel applied to the alveolar socket once after extraction; the second had a eugenol-based paste applied to the alveolar socket once after extraction; and the third group acted as controls, with no treatment. The incidence of alveolar osteitis was recorded for 7 days. We also recorded postoperative pain, inflammation, infection, and wound healing. Nine of the control group (10%) and 2 (2%) of the chlorhexidine group developed alveolar osteitis on the seventh postoperative day. The overall incidence (11/270) was 4%, which is less than reported elsewhere. The distribution of alveolar osteitis among the 3 groups was significant (p=0.002), with the eugenol group having no cases. The chlorhexidine group showed less incidence of alveolar osteitis than other reported studies and also less pain, inflammation, infection, and better wound healing than the control group. We conclude that eugenol was the better of the 2 interventions.

  14. Impact of Helicobacter pylori eradication on refractory thrombocytopenia in patients with chronic HCV awaiting antiviral therapy.

    Science.gov (United States)

    Hanafy, A S; El Hawary, A T; Hamed, E F; Hassaneen, A M

    2016-07-01

    The possibility of delaying treatment of HCV due to severe thrombocytopenia is challenging. This study aimed to detect the prevalence of active helicobacter infection as a claimed cause of thrombocytopenia in a cohort of Egyptian patients with chronic active HCV awaiting combined anti-viral therapy. The study included 400 chronic HCV patients with thrombocytopenia. Laboratory investigations included liver function tests, real time quantitative PCR, reticulocytic count, ESR, ANA, bone marrow aspiration, measurement of anti-helicobacter antibodies, and helicobacter stool antigen. Positive cases for active H. pylori were given the standard triple therapy for 2 weeks. Helicobacter stool antigen was detected 4 weeks after termination of therapy and the change in platelet count was detected 1 month after eradication. A total of 248 out of 281 seropositive patients for H. pylori (88.3 %) showed positive stool antigen (p = 0.01). Eradication was achieved in 169 (68.1 %) patients with platelet mean count 114.9 ± 18.8 × 10(3)/μl with highly significant statistical difference from pretreatment value (49.7 ± 9.2 × 10(3)/μl, p = 0.000). Seventy-nine patients were resistant to conventional triple therapy and given a 7-day course of moxifloxacin-based therapy; 61 patients responded (77.1 %) with mean platelet improvement from 76.4 ± 17.4 × 10(3)/μl to 104.2 ± 15.2 × 10(3)/μl (p = 0.000). The non-responders showed no improvement in their platelet count (74.6 ± 20.5 vs. 73.6 ± 15.3 × 10(3)/ul, P = 0.5). Eradication of active H. pylori in HCV augments platelet count and enhances the early start of antiviral therapy. PMID:27180243

  15. Push-out bond strength of MTA HP, a new high-plasticity calcium silicate-based cement.

    Science.gov (United States)

    Silva, Emmanuel Jnl; Carvalho, Nancy Kudsi; Zanon, Mayara; Senna, Plínio Mendes; DE-Deus, Gustavo; Zuolo, Mário Luis; Zaia, Alexandre Augusto

    2016-06-14

    This study was designed to investigate the resistance to dislodgment provided by MTA HP, a new high-plasticity calcium silicate-based cement. Biodentine and White MTA Angelus were used as reference materials for comparison. Three discs 1 ± 0.1 mm thick were obtained from the middle third of the roots of 5 maxillary canines. Three 0.8-mm-wide holes were drilled on the axial surface of each root disc. Standardized irrigation was performed. Then the holes were dried with paper points and filled with one of the three tested cements. The filled dental slices were immersed in a phosphate-buffered saline (PBS) solution (pH 7.2) for 7 days before the push-out assessment. The Kruskal-Wallis test was applied to assess the effect of each endodontic cement on the push-out bond strength. Mann-Whitney with Bonferroni correction was used to isolate the differences. The alpha-type error was set at 0.05. All specimens had measurable push-out values and no premature failure occurred. There were significant differences among the materials (p <0.05). The Biodentine specimens had the highest push-out bond strength values (p < 0.05). MTA HP had significantly higher bond strength than White MTA (p < 0.05). MTA HP showed better push-out bond strength than its predecessor, White MTA; however, Biodentine had higher dislodgment resistance than both MTA formulations. PMID:27305515

  16. Identification of Ustilago cynodontis as a new producer of glycolipid biosurfactants, mannosylerythritol lipids, based on ribosomal DNA sequences.

    Science.gov (United States)

    Morita, Tomotake; Konishi, Masaaki; Fukuoka, Tokuma; Imura, Tomohiro; Kitamoto, Dai

    2008-01-01

    Mannosylerythritol lipids (MELs) are one of the most promising glycolipid biosurfactants known because of their multifunctionality and biocompatibility. The search for novel producers of MELs was undertaken based on the analysis of ribosomal DNA sequences on basidiomycetous yeasts. The bermuda grass smut fungus Ustilago cynodontis NBRC 7530, which taxonomically relates to Pseudozyma shanxiensis known as a MEL-C producer, was found to accumulate glycolipids in the cultured medium. Under a shake flask culture with soybean oil, the amount of the glycolipids was 1.4 g/L for 7 days at 25 degrees C. As a result of the structural characterization, the main glycolipids was identified as 4-O-[(4'-O-acetyl-3'-O-alka(e)noyl-2'-O-butanoyl)-beta-D-mannopyranosyl]-D-erythritol, and the major fatty acids were C(14) and C(16) ones. The glycolipid was highly hydrophilic MEL-C, and very similar to those produced by P. shanxiensis. The fungi of the genus Ustilago are thus likely to be potential producers of MELs as well as the yeasts of the genus Pseudozyma. PMID:18781055

  17. Reliability of a Cycle Ergometer Peak Power Test in Running-based Team Sport Athletes: A Technical Report.

    Science.gov (United States)

    Wehbe, George M; Gabbett, Tim J; Hartwig, Timothy B; Mclellan, Christopher P

    2015-07-01

    Given the importance of ensuring athletes train and compete in a nonfatigued state, reliable tests are required to regularly monitor fatigue. The purpose of this study was to investigate the reliability of a cycle ergometer to measure peak power during short maximal sprint cycle efforts in running-based team sport athletes. Fourteen professional male Australian rules footballers performed a sprint cycle protocol during 3 separate trials, with each trial separated by 7 days. The protocol consisted of a standardized warm-up, a maximal 6-second sprint cycle effort, a 1-minute active recovery, and a second maximal 6-second sprint cycle effort. Peak power was recorded as the highest power output of the 2 sprint cycle efforts. Absolute peak power (mean ± SD) was 1502 ± 202, 1498 ± 191, and 1495 ± 210 W for trials 1, 2, and 3, respectively. The mean coefficient of variation, intraclass correlation coefficient, and SE of measurement for peak power between trials was 3.0% (90% confidence intervals [CIs] = 2.5-3.8%), 0.96 (90% CIs = 0.91-0.98), and 39 W, respectively. The smallest worthwhile change for relative peak power was 6.0%, which equated to 1.03 W·kg⁻¹. The cycle ergometer sprint test protocol described in this study is highly reliable in elite Australian rules footballers and can be used to track meaningful changes in performance over time, making it a potentially useful fatigue-monitoring tool.

  18. Residual Monomer Content and Its Release into Water from the Denture Base Nanocomposite Using Organic Montmorillonite as Reinforcement

    Institute of Scientific and Technical Information of China (English)

    LI Hongbo; ZHANG Chao; LI Zhian; WANG Yining; XIAO Qun

    2008-01-01

    A novel kind of denture base nanocomposite was prepared by polymethyl methyacrylate(PMMA) and cethyltrimethylammonium bromide modified organic montmorillonite (OMMT).The dispersion of montmorillonite in the polymer matrix was characterized by x-ray diffraction (XRD) and transimission electron microscope (TEM).The content of residual MMA in nanocomposites and the amount of MMA released to water from nanocomposites were determined by gas chromatography (GC).The analysis of TEM and XRD showed that exfoliated-intercalated and intercalated nanocomposites were formed when the content of OMMT was 3% and 5% in the PMMA powder respectively.The results of GC showed that the residual MMA increased with the increase of OMMT content in the polymer matrix.After 7 days in water,the amount of MMA released into water from the nanocomposites tended to be stable.The results of one-way ANOVA and t-test showed that OMMT gave a significant increase of residual MMA concentration (p<0.05) in nanocomposites.

  19. Hydrological response to climate change in the Lesse and the Vesdre catchments: contribution of a physically based model (Wallonia, Belgium

    Directory of Open Access Journals (Sweden)

    A. Bauwens

    2011-06-01

    Full Text Available The Meuse is an important rain-fed river in North-Western Europe. Nine million people live in its catchment, split over five countries. Projected changes in precipitation and temperature characteristics due to climate change would have a significant impact on the Meuse River and its tributaries. In this study, we focused on the impacts of climate change on the hydrology of two sub-catchments of the Meuse in Belgium, the Lesse and the Vesdre, placing the emphasis on the water-soil-plant continuum in order to highlight the effects of climate change on plant growth, and water uptake on the hydrology of two sub-catchments. These effects were studied using two climate scenarios and a physically based distributed model, which reflects the water-soil-plant continuum. Our results show that the vegetation will evapotranspirate between 10 and 17 % less at the end of the century because of water scarcity in summer, even if the root development is better under climate change conditions. In the low scenario, the mean minimal 7 days discharge value could decrease between 19 and 24 % for a two year return period, and between 20 and 35 % for a fifty year return period. It will lead to rare but severe drought in rivers, with potentially huge consequences on water quality.

  20. Push-out bond strength of MTA HP, a new high-plasticity calcium silicate-based cement.

    Science.gov (United States)

    Silva, Emmanuel Jnl; Carvalho, Nancy Kudsi; Zanon, Mayara; Senna, Plínio Mendes; DE-Deus, Gustavo; Zuolo, Mário Luis; Zaia, Alexandre Augusto

    2016-06-14

    This study was designed to investigate the resistance to dislodgment provided by MTA HP, a new high-plasticity calcium silicate-based cement. Biodentine and White MTA Angelus were used as reference materials for comparison. Three discs 1 ± 0.1 mm thick were obtained from the middle third of the roots of 5 maxillary canines. Three 0.8-mm-wide holes were drilled on the axial surface of each root disc. Standardized irrigation was performed. Then the holes were dried with paper points and filled with one of the three tested cements. The filled dental slices were immersed in a phosphate-buffered saline (PBS) solution (pH 7.2) for 7 days before the push-out assessment. The Kruskal-Wallis test was applied to assess the effect of each endodontic cement on the push-out bond strength. Mann-Whitney with Bonferroni correction was used to isolate the differences. The alpha-type error was set at 0.05. All specimens had measurable push-out values and no premature failure occurred. There were significant differences among the materials (p Biodentine specimens had the highest push-out bond strength values (p Biodentine had higher dislodgment resistance than both MTA formulations.

  1. In situ-forming click-crosslinked gelatin based hydrogels for 3D culture of thymic epithelial cells.

    Science.gov (United States)

    Truong, Vinh X; Hun, Michael L; Li, Fanyi; Chidgey, Ann P; Forsythe, John S

    2016-07-21

    Hydrogels prepared from naturally derived gelatin can provide a suitable environment for cell attachment and growth, making them favourable materials in tissue engineering. However, physically crosslinked gelatin hydrogels are not stable under physiological conditions while chemical crosslinking of gelatin by radical polymerization may be harmful to cells. In this study, we attached the norbornene functional group to gelatin, which was subsequently crosslinked with a polyethylene glycol (PEG) linker via the nitrile oxide-norbornene click reaction. The rapid crosslinking process allows the hydrogel to be formed within minutes of mixing the polymer solutions under physiological conditions, allowing the gels to be used as injectable materials. The hydrogels properties including mechanical strength, swelling and degradation, can be tuned by changing either the ratio of the reacting groups or the total concentration of the polymer precursors. Murine embryonic fibroblastic cells cultured in soft gels (2 wt% of gelatin and 1 wt% of PEG linker) demonstrated high cell viability as well as similar phenotypic profiles (PDGFRα and MTS15) to Matrigel cultures over 5 days. Thymic epithelial cell and fibroblast co-cultures produced epithelial colonies in these gels following 7 days incubation. These studies demonstrate that gelatin based hydrogels, prepared using "click" crosslinking, provide a robust cell culture platform with retained benefits of the gelatin material, and are therefore suitable for use in various tissue engineering applications. PMID:27217071

  2. Improving the stability of chitosan-gelatin-based hydrogels for cell delivery using transglutaminase and controlled release of doxycycline.

    Science.gov (United States)

    Tormos, Christian J; Abraham, Carol; Madihally, Sundararajan V

    2015-12-01

    Although local cell delivery is an option to repair tissues, particularly using chitosan-based hydrogels, significant attrition of injected cells prior to engraftment has been a problem. To address this problem, we explored the possibility of stabilizing the chitosan-gelatin (CG) injectable hydrogels using (1) controlled release of doxycycline (DOX) to prevent premature degradation due to increased gelatinase activity (MMP-2 and MMP-9), and (2) transglutaminase (TG) to in situ cross-link gelatin to improve the mechanical stability. We prepared DOX-loaded PLGA nanoparticles, loaded into the CG hydrogels, measured DOX release for 5 days, and modeled using a single-compartmental assumption. Next, we assessed the influence of TG and DOX on hydrogel compression properties by incubating hydrogels for 7 days in PBS. We evaluated the effect of these changes on retention of fibroblasts and alterations in MMP-2/MMP-9 activity by seeding 500,000 fibroblasts for 5 days. These results showed that 90 % of DOX released from cross-linked CG hydrogels after 4 days, unlike CG hydrogels where 90 % of DOX was released within the first day. Addition of TG enhanced the CG hydrogel stability significantly. More than 60 % of seeded fibroblasts were recovered from the CG-TG hydrogels at day 5, unlike 40 % recovered from CG-hydrogels. Inhibition of MMP-2/MMP-9 were observed. In summary, controlled release of DOX from CG hydrogels cross-linked with TG shows a significant potential as a carrier for cell delivery.

  3. Application of visible light-based projection stereolithography for live cell-scaffold fabrication with designed architecture.

    Science.gov (United States)

    Lin, Hang; Zhang, Dongning; Alexander, Peter G; Yang, Guang; Tan, Jian; Cheng, Anthony Wai-Ming; Tuan, Rocky S

    2013-01-01

    One-step scaffold fabrication with live cell incorporation is a highly desirable technology for tissue engineering and regeneration. Projection stereolithography (PSL) represents a promising method owing to its fine resolution, high fabrication speed and computer-aided design (CAD) capabilities. However, the majority of current protocols utilize water-insoluble photoinitiators that are incompatible with live cell-fabrication, and ultraviolet (UV) light that is damaging to the cellular DNA. We report here the development of a visible light-based PSL system (VL-PSL), using lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as the initiator and polyethylene glycol diacrylate (PEGDA) as the monomer, to produce hydrogel scaffolds with specific shapes and internal architectures. Furthermore, live human adipose-derived stem cells (hADSCs) were suspended in PEGDA/LAP solution during the PSL process, and were successfully incorporated within the fabricated hydrogel scaffolds. hADSCs in PEG scaffolds showed high viability (>90%) for up to 7 days after fabrication as revealed by Live/Dead staining. Scaffolds with porous internal architecture retained higher cell viability and activity than solid scaffolds, likely due to increased oxygen and nutrients exchange into the interior of the scaffolds. The VL-PSL should be applicable as an efficient and effective tissue engineering technology for point-of-care tissue repair in the clinic.

  4. Evaluation of tetrazolium-based semiautomatic colorimetric assay for measurement of human antitumor cytotoxicity

    Energy Technology Data Exchange (ETDEWEB)

    Heo, D.S.; Park, J.G.; Hata, K.; Day, R.; Herberman, R.B.; Whiteside, T.L. (Univ. of Pittsburgh School of Medicine, PA (USA))

    1990-06-15

    A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-based colorimetric assay was developed and compared with 51Cr release from different adherent tumor cell targets (human squamous cell carcinoma lines of the head and neck established in our laboratory, melanoma, and colorectal carcinoma) using 5-7-day human lymphokine-activated killer cells and monocyte-depleted peripheral blood lymphocytes as effectors. With adherent tumor cell targets, MTT colorimetry was more sensitive than the 51Cr release assay in measuring the antitumor activity of effectors: median, 4385 (range, 988-8144) versus median, 1061 (range, 582-7294) lytic units (the number of effector cells required to lyse 20% of 5 x 10(3) targets)/10(7) effectors (P less than 0.01). Background effects (without effector cells) were comparable in 4-h assays (9% versus 10%) between MTT colorimetry and 51Cr release. In 24-h assays, MTT colorimetry showed higher antitumor activity (70-100% versus 40-60% lysis at 1:1 effector:target cell ratio) but lower background effects (6% versus 38%) than 51Cr release assay. Thus, MTT colorimetry was more sensitive, did not use radiolabeled targets, required fewer effector cells, and was easier, less expensive, and better adaptable to serial monitoring of effector cell function in cancer patients. This colorimetric assay is especially well suited to adherent tumor cell targets. The use of adherent tumor cell monolayers, as opposed to trypsinized single cell suspensions, provides an opportunity to measure interactions of effector cells with enzymatically unaltered solid tumor targets. Because of the greater sensitivity of the colorimetric assay, the transformation of MTT data into lytic units, as commonly used for 51Cr release assays, required an adjustment to avoid the extrapolation based on the exponential fit equation.

  5. Assessing uncertainties in superficial water provision by different bootstrap-based techniques

    Science.gov (United States)

    Rodrigues, Dulce B. B.; Gupta, Hoshin V.; Mendiondo, Eduardo Mario

    2014-05-01

    An assessment of water security can incorporate several water-related concepts, characterizing the interactions between societal needs, ecosystem functioning, and hydro-climatic conditions. The superficial freshwater provision level depends on the methods chosen for 'Environmental Flow Requirement' estimations, which integrate the sources of uncertainty in the understanding of how water-related threats to aquatic ecosystem security arise. Here, we develop an uncertainty assessment of superficial freshwater provision based on different bootstrap techniques (non-parametric resampling with replacement). To illustrate this approach, we use an agricultural basin (291 km2) within the Cantareira water supply system in Brazil monitored by one daily streamflow gage (24-year period). The original streamflow time series has been randomly resampled for different times or sample sizes (N = 500; ...; 1000), then applied to the conventional bootstrap approach and variations of this method, such as: 'nearest neighbor bootstrap'; and 'moving blocks bootstrap'. We have analyzed the impact of the sampling uncertainty on five Environmental Flow Requirement methods, based on: flow duration curves or probability of exceedance (Q90%, Q75% and Q50%); 7-day 10-year low-flow statistic (Q7,10); and presumptive standard (80% of the natural monthly mean ?ow). The bootstrap technique has been also used to compare those 'Environmental Flow Requirement' (EFR) methods among themselves, considering the difference between the bootstrap estimates and the "true" EFR characteristic, which has been computed averaging the EFR values of the five methods and using the entire streamflow record at monitoring station. This study evaluates the bootstrapping strategies, the representativeness of streamflow series for EFR estimates and their confidence intervals, in addition to overview of the performance differences between the EFR methods. The uncertainties arisen during EFR methods assessment will be

  6. Evaluation of Bias Correction Method for Satellite-Based Rainfall Data

    Directory of Open Access Journals (Sweden)

    Haris Akram Bhatti

    2016-06-01

    Full Text Available With the advances in remote sensing technology, satellite-based rainfall estimates are gaining attraction in the field of hydrology, particularly in rainfall-runoff modeling. Since estimates are affected by errors correction is required. In this study, we tested the high resolution National Oceanic and Atmospheric Administration’s (NOAA Climate Prediction Centre (CPC morphing technique (CMORPH satellite rainfall product (CMORPH in the Gilgel Abbey catchment, Ethiopia. CMORPH data at 8 km-30 min resolution is aggregated to daily to match in-situ observations for the period 2003–2010. Study objectives are to assess bias of the satellite estimates, to identify optimum window size for application of bias correction and to test effectiveness of bias correction. Bias correction factors are calculated for moving window (MW sizes and for sequential windows (SW’s of 3, 5, 7, 9, …, 31 days with the aim to assess error distribution between the in-situ observations and CMORPH estimates. We tested forward, central and backward window (FW, CW and BW schemes to assess the effect of time integration on accumulated rainfall. Accuracy of cumulative rainfall depth is assessed by Root Mean Squared Error (RMSE. To systematically correct all CMORPH estimates, station based bias factors are spatially interpolated to yield a bias factor map. Reliability of interpolation is assessed by cross validation. The uncorrected CMORPH rainfall images are multiplied by the interpolated bias map to result in bias corrected CMORPH estimates. Findings are evaluated by RMSE, correlation coefficient (r and standard deviation (SD. Results showed existence of bias in the CMORPH rainfall. It is found that the 7 days SW approach performs best for bias correction of CMORPH rainfall. The outcome of this study showed the efficiency of our bias correction approach.

  7. Novel gut-based pharmacology of metformin in patients with type 2 diabetes mellitus.

    Directory of Open Access Journals (Sweden)

    Antonella Napolitano

    Full Text Available Metformin, a biguanide derivate, has pleiotropic effects beyond glucose reduction, including improvement of lipid profiles and lowering microvascular and macrovascular complications associated with type 2 diabetes mellitus (T2DM. These effects have been ascribed to adenosine monophosphate-activated protein kinase (AMPK activation in the liver and skeletal muscle. However, metformin effects are not attenuated when AMPK is knocked out and intravenous metformin is less effective than oral medication, raising the possibility of important gut pharmacology. We hypothesized that the pharmacology of metformin includes alteration of bile acid recirculation and gut microbiota resulting in enhanced enteroendocrine hormone secretion. In this study we evaluated T2DM subjects on and off metformin monotherapy to characterize the gut-based mechanisms of metformin. Subjects were studied at 4 time points: (i at baseline on metformin, (ii 7 days after stopping metformin, (iii when fasting blood glucose (FBG had risen by 25% after stopping metformin, and (iv when FBG returned to baseline levels after restarting the metformin. At these timepoints we profiled glucose, insulin, gut hormones (glucagon-like peptide-1 (GLP-1, peptide tyrosine-tyrosine (PYY and glucose-dependent insulinotropic peptide (GIP and bile acids in blood, as well as duodenal and faecal bile acids and gut microbiota. We found that metformin withdrawal was associated with a reduction of active and total GLP-1 and elevation of serum bile acids, especially cholic acid and its conjugates. These effects reversed when metformin was restarted. Effects on circulating PYY were more modest, while GIP changes were negligible. Microbiota abundance of the phylum Firmicutes was positively correlated with changes in cholic acid and conjugates, while Bacteroidetes abundance was negatively correlated. Firmicutes and Bacteroidetes representation were also correlated with levels of serum PYY. Our study suggests that

  8. Multi-model ensemble-based probabilistic prediction of tropical cyclogenesis using TIGGE model forecasts

    Science.gov (United States)

    Jaiswal, Neeru; Kishtawal, C. M.; Bhomia, Swati; Pal, P. K.

    2016-02-01

    An extended range tropical cyclogenesis forecast model has been developed using the forecasts of global models available from TIGGE portal. A scheme has been developed to detect the signatures of cyclogenesis in the global model forecast fields [i.e., the mean sea level pressure and surface winds (10 m horizontal winds)]. For this, a wind matching index was determined between the synthetic cyclonic wind fields and the forecast wind fields. The thresholds of 0.4 for wind matching index and 1005 hpa for pressure were determined to detect the cyclonic systems. These detected cyclonic systems in the study region are classified into different cyclone categories based on their intensity (maximum wind speed). The forecasts of up to 15 days from three global models viz., ECMWF, NCEP and UKMO have been used to predict cyclogenesis based on multi-model ensemble approach. The occurrence of cyclonic events of different categories in all the forecast steps in the grided region (10 × 10 km2) was used to estimate the probability of the formation of cyclogenesis. The probability of cyclogenesis was estimated by computing the grid score using the wind matching index by each model and at each forecast step and convolving it with Gaussian filter. The proposed method is used to predict the cyclogenesis of five named tropical cyclones formed during the year 2013 in the north Indian Ocean. The 6-8 days advance cyclogenesis of theses systems were predicted using the above approach. The mean lead prediction time for the cyclogenesis event of the proposed model has been found as 7 days.

  9. Effect of leaching residual methyl methacrylate concentrations on in vitro cytotoxicity of heat polymerized denture base acrylic resin processed with different polymerization cycles

    Directory of Open Access Journals (Sweden)

    Canan Bural

    2011-08-01

    Full Text Available OBJECTIVES: Residual methyl methacrylate (MMA may leach from the acrylic resin denture bases and have adverse effects on the oral mucosa. This in vitro study evaluated and correlated the effect of the leaching residual MMA concentrations ([MMA]r on in vitro cytotoxicity of L-929 fibroblasts. MATERIAL AND METHODS: A total of 144 heat-polymerized acrylic resin specimens were fabricated using 4 different polymerization cycles: (1 at 74ºC for 9 h, (2 at 74ºC for 9 h and terminal boiling (at 100ºC for 30 min, (3 at 74ºC for 9 h and terminal boiling for 3 h, (4 at 74ºC for 30 min and terminal boiling for 30 min. Specimens were eluted in a complete cell culture medium at 37ºC for 1, 2, 5 and 7 days. [MMA]r in eluates was measured using high-performance liquid chromatography. In vitro cytotoxicity of eluates on L-929 fibroblasts was evaluated by means of cell proliferation using a tetrazolium salt XTT (sodium 3´-[1-phenyl-aminocarbonyl-3,4-tetrazolium]bis(4-methoxy-6-nitrobenzenesulphonic acid assay. Differences in [MMA]r of eluates and cell proliferation values between polymerization cycles were statistically analyzed by Kruskal-Wallis, Friedman and Dunn's multiple comparison tests. The correlation between [MMA]r of eluates and cell proliferation was analyzed by Pearson's correlation test (p<0.05. RESULTS: [MMA]r was significantly (p<0.001 higher in eluates of specimens polymerized with cycle without terminal boiling after elution of 1 and 2 days. Cell proliferation values for all cycles were significantly (p<0.01 lower in eluates of 1 day than those of 2 days. The correlation between [MMA]r and cell proliferation values was negative after all elution periods, showing significance (p<0.05 for elution of 1 and 2 days. MMA continued to leach from acrylic resin throughout 7 days and leaching concentrations markedly reduced after elution of 1 and 2 days. CONCLUSION: Due to reduction of leaching residual MMA concentrations, use of terminal boiling in

  10. Foundation: Transforming data bases into knowledge bases

    Science.gov (United States)

    Purves, R. B.; Carnes, James R.; Cutts, Dannie E.

    1987-01-01

    One approach to transforming information stored in relational data bases into knowledge based representations and back again is described. This system, called Foundation, allows knowledge bases to take advantage of vast amounts of pre-existing data. A benefit of this approach is inspection, and even population, of data bases through an intelligent knowledge-based front-end.

  11. Fermentation Process of Cocoa Based on Optimum Condition of Pulp PectinDepolymerization by Endogenous Pectolityc Enzymes

    Directory of Open Access Journals (Sweden)

    G.P. Ganda-Putra

    2010-08-01

    Full Text Available Pulp degradation during cocoa fermentation can be carried out by depolymerization process of pulp pectin using endogenous pectolytic enzymes at optimum condition. The objectives of this research were to study the effect of fermentation process based on optimum condition in terms of temperature and pH of pulp pectin depolymerization using endogenous pectolytic enzymes polygalakturonase (PG and pectin metyl esterase (PME and fermentation period in cocoa processing on quality characteristics of cocoa beans produced and to study the role of those fermentation process in reducing fermentation time to produce cocoa beans with standard quality. This research used split plot design, with treatments of process condition of cocoa fermentation as main plot and fermentation period as split plot. Treatment of process condition of cocoa fermentation consisted of optimum condition for pulp pectin depolymerization by PGs (temperature 47.5OC; initial pulp pH 4.6; optimum condition of depolymerization on sequence depolymerization by PGs (temperature 48.5OC; initial pulp pH 8.0 during 1 day; last temperature 47.5OC; initial pulp pH 4.6 during 6 days, and natural fermentation process a control. While treatment of fermentation period consisted of 0, 1, 2, 3, 4, 5, 6 and 7 days. Evaluation of fermentation period was carried out based on pursuant to criteria of unfermented beans content and fermentation index. The results showed that process condition and fermentation time of cocoa affected quality characteristic of cocoa beans produced. Period of cocoa fermentation process based on optimum condition for pulp pectin depolymerization using endogenous pectolytic enzymes was 2 days shorter compared to natural fermentation. Cocoa beans quality of grade I and II were obtained from fermentation time of 4 and 2 days, respectively, using fermentation process based on optimum condition of pulp pectin depolymerization using endogenous pectolytic enzymes, whereas 6 and 4 days

  12. Assessing the Effect of Composting Cassava Peel Based Substrates on the Yield, Nutritional Quality, and Physical Characteristics of Pleurotus ostreatus (Jacq. ex Fr. Kummer

    Directory of Open Access Journals (Sweden)

    N. K. Kortei

    2014-01-01

    Full Text Available Cassava peel based substrate formulations as an alternative substrate were used to grow mushrooms. The effect of two compost heights, three composting periods on the mycelia growth, physical characteristics, yield, and nutritional qualities of Pleurotus ostreatus (Jacq. ex Fr. Kummer was studied. Mean mycelia growth of 16.2 cm after a period of seven (7 weeks was the best for 1.5 m compost height. Cap diameter and stipe length differed significantly (P<0.05 with the compost heights (0.8 m and 1.5 m. The yield on compost height of 1.5 m, composted for 5 days, differed significantly (P<0.05 from that of 0.8 m and gave increasing yields as follows: cassava peels and manure, cassava peels only, cassava peels and corn cobs (1 : 1 ratio, and cassava peels and corn cobs (1 : 1 ratio with chicken manure. Composting periods (3 and 7 days gave varying yields depending on the compost height. Based on the findings an interaction of 1.5 m compost height and 5 days composting period on cassava peels and corncobs (1 : 1 ratio with chicken manure produced the best results. The nutritional quality of the mushrooms also differed significantly (P<0.05, indicating that cassava peels could be used as a possible substrate in cultivation of mushroom.

  13. Mortality impact of positive blood cultures in patients with suspected community-acquired bacteraemia. A Danish population-based cohort study

    DEFF Research Database (Denmark)

    Søgaard, Mette; Nørgaard, Mette; Sørensen, Henrik Toft;

    2009-01-01

    the prognostic impact of bacteraemia persisted throughout the follow-up period (0-7 days: MRR = 1.7 (95% CI 1.2-2.6); 8-30 days: MRR = 1.2 (95% CI 0.7-1.9); 31-180 days: MRR = 1.6 (95% CI 1.0-2.1)). Conclusion: Positive blood culture is a predictor of mortality in patients with suspected bacteraemia.......Objective: We examined the prognostic impact of positive blood cultures compared to negative cultures on mortality in patients, who had blood cultures obtained during the first 72 hours of admission to a medical ward. Methods: We conducted this population-based cohort study of adults (n = 20...... from medical databases. Positive cultures were defined as those with growth of one or more pathogen given an aetiological role based on joint clinical and microbiological assessment. Mortality within 180 days following the date of first blood culture was determined through the Danish Civil Registration...

  14. Reducing Environmental Tobacco Smoke Exposure of Preschool Children: A Randomized Controlled Trial of Class-Based Health Education and Smoking Cessation Counseling for Caregivers

    Directory of Open Access Journals (Sweden)

    Yun Wang

    2015-01-01

    Full Text Available Objectives: To assess counseling to caregivers and classroom health education interventions to reduce environmental tobacco smoke exposure of children aged 5–6 years in China. Methods: In a randomized controlled trial in two preschools in Changsha, China, 65 children aged 5–6 years old and their smoker caregivers (65 were randomly assigned to intervention (n = 33 and control (no intervention groups (n = 32. In the intervention group, caregivers received self-help materials and smoking cessation counseling from a trained counselor, while their children were given classroom-based participatory health education. Children’s urinary cotinine level and the point prevalence of caregiver quitting were measured at baseline and after 6 months. Results: At the 6-month follow-up, children’s urinary cotinine was significantly lower (Z = –3.136; p = 0.002 and caregivers’ 7-day quit rate was significantly higher (34.4% versus 0% (p < 0.001; adjusted OR = 1.13; 95% CI: 1.02–1.26 in the intervention than control group. Conclusions: Helping caregivers quitting smoke combined with classroom-based health education was effective in reducing children’s environmental tobacco smoke exposure. Larger-scale trials are warranted.

  15. Assessing the Effect of Composting Cassava Peel Based Substrates on the Yield, Nutritional Quality, and Physical Characteristics of Pleurotus ostreatus (Jacq. ex Fr.) Kummer.

    Science.gov (United States)

    Kortei, N K; Dzogbefia, V P; Obodai, M

    2014-01-01

    Cassava peel based substrate formulations as an alternative substrate were used to grow mushrooms. The effect of two compost heights, three composting periods on the mycelia growth, physical characteristics, yield, and nutritional qualities of Pleurotus ostreatus (Jacq. ex Fr.) Kummer was studied. Mean mycelia growth of 16.2 cm after a period of seven (7) weeks was the best for 1.5 m compost height. Cap diameter and stipe length differed significantly (P corn cobs (1 : 1 ratio), and cassava peels and corn cobs (1 : 1 ratio) with chicken manure. Composting periods (3 and 7 days) gave varying yields depending on the compost height. Based on the findings an interaction of 1.5 m compost height and 5 days composting period on cassava peels and corncobs (1 : 1 ratio) with chicken manure produced the best results. The nutritional quality of the mushrooms also differed significantly (P cultivation of mushroom. PMID:25580299

  16. Freeze-Thaw Performance and Moisture-Induced Damage Resistance of Base Course Stabilized with Slow Setting Bitumen Emulsion-Portland Cement Additives

    Directory of Open Access Journals (Sweden)

    Mojtaba Shojaei Baghini

    2015-01-01

    Full Text Available Freeze-thaw (FT cycles and moisture susceptibility are important factors influencing the geotechnical characteristics of soil-aggregates. Given the lack of published information on the behavior of cement-bitumen emulsion-treated base (CBETB under environmental conditions, especially freezing and thawing, this study investigated the effects of these additives on the CBETB performance. The primary goal was to evaluate the resistance of CBETB to moisture damage by performing FT, Marshall conditioning, and AASHTO T-283 tests and to evaluate the long-term stripping susceptibility of CBETB while also predicting the liquid antistripping additives to assess the mixture’s durability and workability. Specimens were stabilized with Portland cement (0%–6%, bitumen emulsion (0%–5%, and Portland cement-bitumen emulsion mixtures and cured for 7 days, and their short- and long-term performances were studied. Evaluation results of both the Marshall stability ratio and the tensile strength ratio show that the additions of additives increase the resistance of the mixtures to moisture damage. Results of durability tests performed for determining the resistance of compacted specimens to repeated FT cycles indicate that the specimen with the 4% cement-3% bitumen emulsion mixture significantly improves water absorption, volume changes, and weight losses. This indicates the effectiveness of this additive as a road base stabilizer with excellent engineering properties for cold regions.

  17. Results of the PAS Study: A Randomized Controlled Trial Evaluating the Effectiveness of a Web-Based Multiple Tailored Smoking Cessation Program Combined With Tailored Counseling by Practice Nurses.

    Science.gov (United States)

    Smit, E S; Candel, M J J M; Hoving, C; de Vries, H

    2016-09-01

    This study investigated the effects of Web-based multiple computer tailoring and counseling by a practice nurse (MTC) compared with computer tailoring without counseling (MT) and usual care (UC) on smoking cessation rates, via a randomized controlled trial with 414 Dutch adult smokers, recruited by 91 practice nurses from May 2009 to June 2010. Logistic multilevel regression analyses were conducted with 24-hour point prevalence, 7-day point prevalence, and prolonged abstinence after 6 and 12 months as dependent variables and experimental condition as the independent variable. After 6 and 12 months, 38% and 56% of respondents were followed up, respectively. At both follow-ups, no main effects of the interventions could be identified when comparing them with care as usual and with each other-neither in analyses using available data nor in analyses using a negative scenario in which respondents lost to follow-up were considered to still be smoking. A Web-based multiple computer-tailored smoking cessation program combined with a single face-to-face counseling session by a practice nurse may not be more effective than this computer-tailored program alone or than usual smoking cessation care in the general practice setting. Yet before concluding that the addition of counseling to Web-based computer tailoring cannot be successful, more research needs to be conducted to identify the optimal number of counseling sessions to be combined with the Web-based program and to how to best attune the two modalities. PMID:26934538

  18. 头孢唑啉钠与莫西沙星配伍稳定性观察%Compatibility of cefazoin sodium combined with moxifloxacin

    Institute of Scientific and Technical Information of China (English)

    宋畅; 张丽梅; 路凯华

    2009-01-01

    莫西沙星(Moxifloxacin)为第四代新型8-甲氧基氟喹诺酮类抗菌药物,目前在我国上市的有莫西沙星片、胶囊及其注射液。本品既保持了早期喹诺酮类药物对G-菌的抗菌活性,同时又增强了对G+球菌,非典型病原体如支原体、衣原体,军团菌属和厌氧菌的抗菌活性,具有广谱、高效、低毒、呈低水平耐药、无光毒性等特点;

  19. Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells.

    Science.gov (United States)

    Garcia, Lucas da Fonseca Roberti; Santos, Alailson Domingos dos; Moraes, João Carlos Silos; Costa, Carlos Alberto de Souza

    2016-01-01

    The present study aimed at evaluating the cytotoxic effects of a novel cement called CER on periodontal fibroblast-like cells of mice (MDPL-20), in comparison with different formulations of Mineral Trioxide Aggregate (MTA), by means of the cell viability test (MTT) and cell morphology analysis. Thirty-two round-shaped samples were fabricated with the following cements: white MTA, white and gray CER and experimental white MTA. The samples were immersed in serum-free culture medium for 24 hours or 7 days (n = 16). The extracts (culture medium + components released from the cements) were applied for 24 hours to previously cultured cells (40.000 cells/cm2) in the wells of 24-well plates. Cells seeded in complete culture medium were used as a negative control. Cell viability was assessed using the MTT assay. Two samples of each cement were used for cell morphology analysis by Scanning Electron Microscopy (SEM). The extracts obtained at the 7-day period presented higher cytotoxicity compared with the 24-hour period (p 0.05). However, at the 7-day period, the experimental white MTA presented no significant difference in comparison with the other cements (p > 0.05). At the 7-day period, CER cement presented cytotoxic effects on fibroblast-like cells, similar to different MTA formulations. However, the immersion period in the culture medium influenced the cytotoxicity of the cements, which was greater for CER cement at 24 hours.

  20. Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells

    Directory of Open Access Journals (Sweden)

    Lucas da Fonseca Roberti GARCIA

    2016-01-01

    Full Text Available Abstract The present study aimed at evaluating the cytotoxic effects of a novel cement called CER on periodontal fibroblast-like cells of mice (MDPL-20, in comparison with different formulations of Mineral Trioxide Aggregate (MTA, by means of the cell viability test (MTT and cell morphology analysis. Thirty-two round-shaped samples were fabricated with the following cements: white MTA, white and gray CER and experimental white MTA. The samples were immersed in serum-free culture medium for 24 hours or 7 days (n = 16. The extracts (culture medium + components released from the cements were applied for 24 hours to previously cultured cells (40.000 cells/cm2 in the wells of 24-well plates. Cells seeded in complete culture medium were used as a negative control. Cell viability was assessed using the MTT assay. Two samples of each cement were used for cell morphology analysis by Scanning Electron Microscopy (SEM. The extracts obtained at the 7-day period presented higher cytotoxicity compared with the 24-hour period (p 0.05. However, at the 7-day period, the experimental white MTA presented no significant difference in comparison with the other cements (p > 0.05. At the 7-day period, CER cement presented cytotoxic effects on fibroblast-like cells, similar to different MTA formulations. However, the immersion period in the culture medium influenced the cytotoxicity of the cements, which was greater for CER cement at 24 hours.

  1. Silicon microfluidic flow focusing devices for the production of size-controlled PLGA based drug loaded microparticles.

    Science.gov (United States)

    Keohane, Kieran; Brennan, Des; Galvin, Paul; Griffin, Brendan T

    2014-06-01

    The increasing realisation of the impact of size and surface properties on the bio-distribution of drug loaded colloidal particles has driven the application of micro fabrication technologies for the precise engineering of drug loaded microparticles. This paper demonstrates an alternative approach for producing size controlled drug loaded PLGA based microparticles using silicon Microfluidic Flow Focusing Devices (MFFDs). Based on the precise geometry and dimensions of the flow focusing channel, microparticle size was successfully optimised by modifying the polymer type, disperse phase (Qd) flow rate, and continuous phase (Qc) flow rate. The microparticles produced ranged in sizes from 5 to 50 μm and were highly monodisperse (coefficient of variation <5%). A comparison of Ciclosporin (CsA) loaded PLGA microparticles produced by MFFDs vs conventional production techniques was also performed. MFFDs produced microparticles with a narrower size distribution profile, relative to the conventional approaches. In-vitro release kinetics of CsA was found to be influenced by the production technique, with the MFFD approach demonstrating the slowest rate of release over 7 days (4.99 ± 0.26%). Finally, MFFDs were utilised to produce pegylated microparticles using the block co-polymer, PEG-PLGA. In contrast to the smooth microparticles produced using PLGA, PEG-PLGA microparticles displayed a highly porous surface morphology and rapid CsA release, with 85 ± 6.68% CsA released after 24h. The findings from this study demonstrate the utility of silicon MFFDs for the precise control of size and surface morphology of PLGA based microparticles with potential drug delivery applications.

  2. Re-use of stabilised flue gas ashes from solid waste incineration in cement-treated base layers for pavements.

    Science.gov (United States)

    Cai, Zuansi; Jensen, Dorthe L; Christensen, Thomas H; Bager, Dirch H

    2003-02-01

    Fly ash from coal-burning power plants has been used extensively as a pozzolan and fine filler in concrete for many years. Laboratory experiments were performed investigating the effect of substituting the coal-based fly ash with chemically stabilised flue gas ashes (FGA) from waste incineration. Two types of FGA were treated by the Ferrox-process, which removes the majority of the easily soluble salts in the FGA and provides binding sites for heavy metals in terms of ferrihydrite. Cubes of cement treated base layer materials containing 5% stabilised FGA were cast, sealed and cured for two weeks. Cylinders (diameter 100 mm, length 150 mm) were drilled from these cubes for tank leaching experiments. Duplicate specimens were subject to compression strength testing and to tank leaching experiments. The compressive strength of the CTB fulfilled the Danish requirements for CTB, i.e. strength more than 5 MPa after 7 days. The tank leaching tests revealed that leaching of heavy metals was not significantly affected by the use of chemically stabilised flue gas ashes from waste incineration. Assuming that diffusion controls the leaching process it was calculated that less than 1% of the metals would leach during a 100-year period from a 0.5 m thick concrete slab exposed to water on one side. Leaching of the common ions Ca, Cl, Na and SO4 was increased 3-20 times from the specimens with chemically stabilised flue gas ashes from waste incineration. However, the quantities leached were still modest. These experiments suggest that FGA from waste incineration after Ferrox-treatment could be re-used in CTB without compromising the strength and leaching from the base layer.

  3. Effects of Community-Based Newborn Care Intervention on Neonate Health Status in a District of Tehran (Iran)

    Science.gov (United States)

    Nayeri, Fatemeh; Dalili, Hosein; Shahzadeh Fazeli, Kazem; Delbarpoor Ahmadi, Shahnaz; Akrami, Frozan; Esmailnia, Tahereh; Habibelahi, Abbas; Shariat, Mamak

    2016-01-01

    Objective: To identify the effects of community-based interventions on the Neonatal Health Index in one district of Tehran-Iran. Materials and methods: A community and healthcare center-based study was carried out from January 2011 through September 2014. The population of the study included newborns from mothers residing in the 4th district of Tehran, Iran. Demographic data of mothers and infants were recorded in questionnaires before and after intervention. Interventions were implemented in hospitals, participants' homes, and health centers. The primary outcomes were comparison of mean birth weight, weight gain during the first 3-7 days, first week visit rate, hospitalization rate between the before and after intervention groups. Results: The populations in the before and after intervention groups were 274 and 250, respectively. A significant difference was seen between the gestational ages (P value = 0.007) of the two groups. Mean birth height in the first group was 50.35 ± 3.48 and in the second group was 55 ± 5.32 cm (P value = 0.04). Neonatal complications in the second group were 6.9% lower than in the first group (P value = 0.048). In the first group 41 neonates (15%) were hospitalized in the NICU while in the second group 12 cases (4.8%) were hospitalized (P value = 0.018). Seven cases (2.6%) in the first group and one case (0.4%) in the second group were resuscitated (P value = 0.0001). Conclusion: The results of implementing community-based newborn care strategies witnessed at the first week postnatal visit included improvements inneonatal gestational growth, management of neonates with potentially serious illnesses, diagnosis of warning signs and neonatal care practices.

  4. Design of novel injectable cationic microspheres based on aminated gelatin for prolonged insulin action.

    Science.gov (United States)

    Morimoto, Kazuhiro; Chono, Sumio; Kosai, Tadashi; Seki, Toshinobu; Tabata, Yasuhiko

    2005-07-01

    The aim of this study was to prepare two types of injectable cationized microspheres based on a native gelatin (NGMS) and aminated gelatin with ethylenediamine (CGMS) to prolong the action of insulin. Release of rhodamin B isothiocyanate insulin from CGMS was compared with that from NGMS under in-vitro and in-vivo conditions. Lower release of insulin from CGMS compared with that from NGMS was caused by the suppression of initial release. The disappearance of 125I-insulin from the injection site after intramuscular administration by NGMS and CGMS had a biphasic profile in mice. Almost all the 125I-insulin had disappeared from the injection site one day after administration by NGMS. The remaining insulin at the injection site after administration by CGMS was prolonged, with approximately 59% remaining after one day and 16% after 14 days. The disappearance of CGMS from the injection site was lower than that of NGMS. However, the difference in these disappearance rates was not great compared with those of 125I-insulin from the injection site by NGMS and CGMS. The time course of disappearance of 125I-CGMS from the injection site was similar to that of 125I-insulin by CGMS. The initial hypoglycaemic effect was observed 1 h after administration of insulin by NGMS, thereafter its effect rapidly disappeared. The hypoglycaemic effect was observed 2-4 h after administration by CGMS and continued to be exhibited for 7 days. The prolonged hypoglycaemic action by CGMS depended on the time profiles of the disappearance of insulin from muscular tissues, which occurs due to the enzymatic degradation of CGMS.

  5. Individual-specific transgenerational marking of fish populations based on a barium dual-isotope procedure.

    Science.gov (United States)

    Huelga-Suarez, Gonzalo; Moldovan, Mariella; Garcia-Valiente, America; Garcia-Vazquez, Eva; Alonso, J Ignacio Garcia

    2012-01-01

    The present study focuses on the development and evaluation of an individual-specific transgenerational marking procedure using two enriched barium isotopes, (135)Ba and (137)Ba, mixed at a given and selectable molar ratio. The method is based on the deconvolution of the isotope patterns found in the sample into four molar contribution factors: natural xenon (Xe nat), natural barium (Ba nat), Ba135, and Ba137. The ratio of molar contributions between Ba137 and Ba135 is constant and independent of the contribution of natural barium in the sample. This procedure was tested in brown trout ( Salmo trutta ) kept in captivity. Trout were injected with three different Ba137/Ba135 isotopic signatures ca. 7 months and 7 days before spawning to compare the efficiency of the marking procedure at long and short term, respectively. The barium isotopic profiles were measured in the offspring by means of inductively coupled plasma mass spectrometry. Each of the three different isotopic signatures was unequivocally identified in the offspring in both whole eggs and larvae. For 9 month old offspring, the characteristic barium isotope signatures could also be detected in the otoliths even in the presence of a high and variable amount of barium of natural isotope abundance. In conclusion, it can be stated that the proposed dual-isotope marking is inheritable and can be detected after both long-term and short-term marking. Furthermore, the dual-isotope marking can be made individual-specific, so that it allows identification of offspring from a single individual or a group of individuals within a given fish group.

  6. Mg-Zn based composites reinforced with bioactive glass (45S5) fabricated via powder metallurgy

    Science.gov (United States)

    Ab llah, N.; Jamaludin, S. B.; Daud, Z. C.; Zaludin, M. A. F.

    2016-07-01

    Metallic implants are shifting from bio-inert to bioactive and biodegradable materials. These changes are made in order to improve the stress shielding effect and bio-compatibility and also avoid the second surgery procedure. Second surgery procedure is required if the patient experienced infection and implant loosening. An implant is predicted to be well for 15 to 20 years inside patient body. Currently, magnesium alloys are found to be the new biomaterials because of their properties close to the human bones and also able to degrade in the human body. In this work, magnesium-zinc based composites reinforced with different content (5, 15, 20 wt. %) of bioactive glass (45S5) were fabricated through powder metallurgy technique. The composites were sintered at 450˚C. Density and porosity of the composites were determined using the gas pycnometer. Microstructure of the composites was observed using an optical microscope. In-vitro bioactivity behavior was evaluated in the simulated body fluid (SBF) for 7 days. Fourier Transform Infrared (FTIR) was used to characterize the apatite forming on the samples surface. The microstructure of the composite showed that the pore segregated near the grain boundaries and bioglass clustering was observed with increasing content of bioglass. The true density of the composites increased with the increasing content of bioglass and the highest value of porosity was indicated by the Mg-Zn reinforced with 20 wt.% of bioglass. The addition of bio-glass to the Mg-Zn has also induced the formation of apatite layer after soaking in SBF solution.

  7. Associations of Monitor-Assessed Activity with Performance-Based Physical Function.

    Science.gov (United States)

    Reid, Natasha; Daly, Robin M; Winkler, Elisabeth A H; Gardiner, Paul A; Eakin, Elizabeth G; Owen, Neville; Dunstan, David W; Healy, Genevieve N

    2016-01-01

    The purpose of this study was to investigate the cross-sectional associations of monitor-derived measures of sedentary time and physical activity with performance-based physical function in healthy Australian adults. Data from 602 participants (mean age 58.1±10.0 years; 58% female) from the 2011/12 wave of the Australian Diabetes, Obesity and Lifestyle (AusDiab3) study were analyzed. The thigh-worn activPAL3™ monitor (7-days continuous wear) was used to derive time during waking hours spent: sitting/reclining; standing; and, stepping (overall, and separately as light [<3 METs] and moderate-to-vigorous physical activity [MVPA; ≥3 METs]), and number of sit-stand transitions. Associations of these (in hours/day, or 15 transitions/day) with physical function measures (8ft Timed Up and Go [TUG-8; log-transformed seconds] and Knee Extensor Strength [KES; kg]) were tested via linear regression, adjusting for confounders. Interactions by sex and age-category (<45; 45-54; 55-64; ≥65 years) were tested. In all participants, KES was significantly (p<0.05) associated with stepping and MVPA stepping only; none of the activity measures were associated with TUG-8. However, subgroup analysis revealed that in older adults (≥65 years), TUG-8 was associated with stepping and MVPA stepping (both p<0.05). All associations with sitting time, standing, sit-stand transition and sex interactions were not statistically significant. In summary, sitting time was not significantly associated with impaired muscle strength or gait/mobility in Australian adults aged 36-80 years, but light- to moderate activity (stepping) was positively associated with muscle strength, and gait/mobility in older adults aged ≥65 years. The direction of causation is not known and remains important to investigate considering the high prevalence of both poor function and limited activity in older age. PMID:27073888

  8. Photodegradation of polycyclic aromatic hydrocarbons in soils under a climate change base scenario.

    Science.gov (United States)

    Marquès, Montse; Mari, Montse; Audí-Miró, Carme; Sierra, Jordi; Soler, Albert; Nadal, Martí; Domingo, José L

    2016-04-01

    The photodegradation of polycyclic aromatic hydrocarbons (PAHs) in two typical Mediterranean soils, either coarse- or fine-textured, was here investigated. Soil samples, spiked with the 16 US EPA priority PAHs, were incubated in a climate chamber at stable conditions of temperature (20 °C) and light (9.6 W m(-2)) for 28 days, simulating a climate change base scenario. PAH concentrations in soils were analyzed throughout the experiment, and correlated with data obtained by means of Microtox(®) ecotoxicity test. Photodegradation was found to be dependent on exposure time, molecular weight of each hydrocarbon, and soil texture. Fine-textured soil was able to enhance sorption, being PAHs more photodegraded than in coarse-textured soil. According to the EC50 values reported by Microtox(®), a higher detoxification was observed in fine-textured soil, being correlated with the outcomes of the analytical study. Significant photodegradation rates were detected for a number of PAHs, namely phenanthrene, anthracene, benzo(a)pyrene, and indeno(123-cd)pyrene. Benzo(a)pyrene, commonly used as an indicator for PAH pollution, was completely removed after 7 days of light exposure. In addition to the PAH chemical analysis and the ecotoxicity tests, a hydrogen isotope analysis of benzo(a)pyrene was also carried out. The degradation of this specific compound was associated to a high enrichment in (2)H, obtaining a maximum δ(2)H isotopic shift of +232‰. This strong isotopic effect observed in benzo(a)pyrene suggests that compound-specific isotope analysis (CSIA) may be a powerful tool to monitor in situ degradation of PAHs. Moreover, hydrogen isotopes of benzo(a)pyrene evidenced a degradation process of unknown origin occurring in the darkness.

  9. Photodegradation of polycyclic aromatic hydrocarbons in soils under a climate change base scenario.

    Science.gov (United States)

    Marquès, Montse; Mari, Montse; Audí-Miró, Carme; Sierra, Jordi; Soler, Albert; Nadal, Martí; Domingo, José L

    2016-04-01

    The photodegradation of polycyclic aromatic hydrocarbons (PAHs) in two typical Mediterranean soils, either coarse- or fine-textured, was here investigated. Soil samples, spiked with the 16 US EPA priority PAHs, were incubated in a climate chamber at stable conditions of temperature (20 °C) and light (9.6 W m(-2)) for 28 days, simulating a climate change base scenario. PAH concentrations in soils were analyzed throughout the experiment, and correlated with data obtained by means of Microtox(®) ecotoxicity test. Photodegradation was found to be dependent on exposure time, molecular weight of each hydrocarbon, and soil texture. Fine-textured soil was able to enhance sorption, being PAHs more photodegraded than in coarse-textured soil. According to the EC50 values reported by Microtox(®), a higher detoxification was observed in fine-textured soil, being correlated with the outcomes of the analytical study. Significant photodegradation rates were detected for a number of PAHs, namely phenanthrene, anthracene, benzo(a)pyrene, and indeno(123-cd)pyrene. Benzo(a)pyrene, commonly used as an indicator for PAH pollution, was completely removed after 7 days of light exposure. In addition to the PAH chemical analysis and the ecotoxicity tests, a hydrogen isotope analysis of benzo(a)pyrene was also carried out. The degradation of this specific compound was associated to a high enrichment in (2)H, obtaining a maximum δ(2)H isotopic shift of +232‰. This strong isotopic effect observed in benzo(a)pyrene suggests that compound-specific isotope analysis (CSIA) may be a powerful tool to monitor in situ degradation of PAHs. Moreover, hydrogen isotopes of benzo(a)pyrene evidenced a degradation process of unknown origin occurring in the darkness. PMID:26841292

  10. Iron-phosphate-based chemically bonded phosphate ceramics for mixed waste stabilization

    International Nuclear Information System (INIS)

    In an effort to develop chemically bonded phosphate ceramics for mixed waste stabilization, a collaborative project to develop iron-phosphate based ceramics has been initiated between Argonne National Laboratory and the V. G. Khlopin Radium Institute in St. Petersburg, Russia. The starter powders are oxides of iron that are generated as inexpensive byproduct materials in the iron and steel industry. They contain iron oxides as a mixture of magnetite (Fe3O4) and haematite (Fe2O3). In this initial phase of this project, both of these compounds were investigated independently. Each was reacted with phosphoric acid solution to form iron phosphate ceramics. In the case of magnetite, the reaction was rapid. Adding ash as the waste component containing hazardous contaminants resulted in a dense and hard ceramic rich in glassy phase. On the other hand, the reaction of phosphoric acid solution with a mixture of haematite and ash waste contaminated with cesium and americium was too slow. Samples had to be molded under pressure. They were cured for 2-3 weeks and then hardened by heating at 350 degrees C for 3 h. The resulting ceramics in both cases were subjected to physical tests for measurement of density, open porosity, compression strength, phase analyses using X-ray diffraction and differential thermal analysis, and leaching tests using toxicity characteristic leaching procedure (TCLP) and ANS 16.1 with 7 days of leaching. Using the preliminary information obtained from these tests, we evaluated these materials for stabilization of Department of Energy's mixed waste streams

  11. Effect of Pulmonary Surfactant on Antimicrobial Activity In Vitro

    OpenAIRE

    Schwameis, R; Erdogan-Yildirim, Z.; Manafi, M.; Zeitlinger, M. A.; Strommer, S.; Sauermann, R.

    2013-01-01

    Time-kill curve experiments were performed with linezolid, doripenem, tigecycline, moxifloxacin, and daptomycin against Staphylococcus aureus and with colistin, moxifloxacin, and doripenem against Pseudomonas aeruginosa to evaluate the effect of porcine pulmonary surfactant on antimicrobial activity. Pulmonary surfactant significantly impaired the activities of moxifloxacin and colistin. When antibiotics are being developed for respiratory tract infections, the method described here might be ...

  12. Literature analysis on ADR induced by moxinoxacin in the elderly%莫西沙星玫老年人不良反应文献分析

    Institute of Scientific and Technical Information of China (English)

    郭安平; 唐丽琴

    2012-01-01

    Objective To explore the status quo of ADR induced by moxifloxacin in the elderly.Methods Retrieved from CNKI data-base with“Moxifloxacin”as key words,49 literatures about 73 ADR cases induced by Moxifloxacin in the elderly were statistically ana-lyzed in respects of patients'age and sex,occurrence time of ADR,route of administration,organs and systems involved in ADR and clin-ical manifestation.Etc.Results There were 73 cases of moxifloxacin-induced ADR.63.1%of which were male and 31.5%the elderly (aged from 81 to 90).The ADRs of moxifloxacin mainly appeared as symptoms in nervous system(41.1%),followed by digestive sys-tern(27.4%)and cardiovascular system(15.1%).The main clinical manifestations of ADR in the nervous system were abnormal mental status and epilepsy(40.0%).Conclusion Great importance should be attached to the ADR induced by moxifloxacin in the elderly and occurrence of severe ADR.%目的 了解莫西沙星致老年人不良反应发生的情况.方法 以"莫西沙星"为题名,检索2000-2010年中有关莫西沙星致老年人(≥60岁)不良反应的文献资料49篇,共73例,并按患者性别、ADR发生时间、给药途经、累及系统及临床表现等进行统计分析.结果 引起不良反应以男性居多(63.0%);81-90岁老年人发生不良反应较多(31.5%);多表现为神经系统反应(41.1%)、消化系统反应(27.4%)、心血管系统反应(15.1%),睡眠障碍和烦躁是神经系统不良反应的主要表现(40.0%).结论 临床应重视莫西沙星在老年人中引起的不良反应,警惕严重不良反应的发生.

  13. 基于信息化的医院门急诊流程再造实践%Informatization-Based Practice of BPR for Emergency and Outpatient Departments in Hospital

    Institute of Scientific and Technical Information of China (English)

    黄培

    2014-01-01

    Informatization-based BPR (Business Process Reengineering) for Department of Outpatient and Department of Emergency in the hospital has served as an important move in improving medical attention and simplifying the service process. With basic theoretical illustration of the concept BPR for the two departments in the hospital, Wuxi No.2 Prople’s Hospital was exempliifed in this paper for its successful accomplishment of BPR by establishment of a HIS-based booking platform, an intensive comprehensive inspection service booking platform as well as a queue system, and implementation of the self-service-based treatment-before-payment process and 24-hour&7-day self-help registration service for fees payment.%基于信息化的医院门急诊流程再造是改善医疗服务、精简环节的重要举措。本文阐述医院门诊急流程再造的理论基础,具体介绍我院建立的包括基于HIS的预约平台、基于自助的先诊疗后结算、推行24 h×7自助挂号缴费、建立集约式的综合检查预约平台和排队叫号系统的流程再造。

  14. INVOLUTIVE BASES UNDER COMPOSITION

    Institute of Scientific and Technical Information of China (English)

    Zailiang TANG

    2007-01-01

    In this paper, the behaviors of involutive bases under composition operation are studied.For two kinds of involutive bases, i.e., Pommaret bases, Janet bases, we study their behavior problems under composition. Some further problems are also proposed.

  15. Development of Co-based bulk metallic glasses as potential biomaterials.

    Science.gov (United States)

    Zhou, Zeyan; Wei, Qin; Li, Qiang; Jiang, Bingliang; Chen, You; Sun, Yanfei

    2016-12-01

    A new series of Co80-x-yCrxMoyP14B6 (x=5 y=5; x=5 y=10; x=10 y=10, all values in at.%) bulk metallic glasses (BMGs) with a maximum diameter of 1.5mm has been developed for using them as potential bio-implant materials by a combination of fluxing treatment and J-quenching technique. The performance of the present Co-based BMGs in biomedical implant applications was investigated as compared to the CoCrMo biomedical alloy (ASTM F75) and 316L stainless steel (316L SS). The corrosion behavior of the samples was investigated in both Hank's solution (pH=7.4) and artificial saliva solution (pH=6.3) at 37°C employing electrochemical measurements. The results indicate that the Co-based BMGs exhibit much higher corrosion resistance in the simulated body solutions than that of 316L SS. Compared with the corrosion resistance of ASTM F75, that of Co70Cr5Mo5P14B6 and Co65Cr5Mo10P14B6 BMGs is found to be lower and that of Co60Cr10Mo10P14B6 BMG is higher. The concentrations of Co, Cr, and Mo ions released into the simulated body solutions from our Co-based BMGs after potentiodynamic polarization are significantly lower than that released from ASTM F75. The biocompatibility of the specimens was evaluated using an in vitro test of NIH3T3 cell culture in the specimen extraction media for 1, 3, 5, and 7days, revealing the non-cytotoxicity of the Co-based BMGs towards NIH3T3 cells. Moreover, examinations on the cell adhesion and growth on the surface of the specimens indicate that the Co-based BMGs exhibit better cell viability compared to ASTM F75 and 316L SS biomedical alloys. PMID:27612687

  16. Use of an integrated biomarker-based strategy to evaluate physiological stress responses induced by environmental concentrations of caffeine in the Mediterranean mussel Mytilus galloprovincialis.

    Science.gov (United States)

    Capolupo, Marco; Valbonesi, Paola; Kiwan, Alisar; Buratti, Sara; Franzellitti, Silvia; Fabbri, Elena

    2016-09-01

    The occurrence of caffeine (CF), a biologically active drug, has widely been documented in coastal waters, and whether its environmental concentrations do represent a threat for marine organisms is unclear. The present study aimed at assessing sub-lethal effects induced by a 7-day exposure to environmentally relevant concentrations of CF (5, 50 and 500ng/L) in the Mediterranean mussel, Mytilus galloprovincialis. CF in water and mussel tissues, and a battery of biomarkers, including lysosomal parameters of general stress, oxidative stress responses and endpoints of neurological and genetic damages, were evaluated and tested for significance vs controls (pGST) and catalase was unmodified in gills, while a significant increase of GST activity was observed in digestive gland (at 5 and 500ng/L CF), suggesting the occurrence of GST-mediated phase II detoxifying processes. CF did not induce geno/neurotoxicity, as shown by the lack of effects on primary DNA damages and acetylcholinesterase activity. In line with its high hydrophilicity, CF did not bioaccumulate in mussel tissues. Data were integrated using the Mussel Expert System, which assigned a low stress level to mussels exposed to 500ng/L CF, whereas no alterations of animal health status were highlighted at lower dosages. This study revealed a low profile of toxicity for environmental concentrations of CF, and confirmed the suitability of an integrated biomarker-based approach to provide a comprehensive picture of the degree of stress induced by emerging contaminants in marine invertebrates. PMID:27152995

  17. Analysing the fate of nanopesticides in soil and the applicability of regulatory protocols using a polymer-based nanoformulation of atrazine.

    Science.gov (United States)

    Kah, Melanie; Machinski, Patrick; Koerner, Petra; Tiede, Karen; Grillo, Renato; Fraceto, Leonardo Fernandes; Hofmann, Thilo

    2014-10-01

    For the first time, regulatory protocols defined in the OECD guidelines were applied to determine the fate properties of a nanopesticide in two agricultural soils with contrasting characteristics. The nanoformulation studied had no effect on the degradation kinetics of atrazine indicating that (1) the release of atrazine from the polymer nanocarriers occurred rapidly relative to the degradation kinetics (half-lives 36-53 days) and/or that (2) atrazine associated with the nanocarriers was subject to biotic or abiotic degradation. Sorption coefficients, derived from a batch and a centrifugation technique at a realistic soil-to-solution ratio, were higher for the nanoformulated atrazine than for the pure active ingredient. Results indicate that the nanoformulation had an effect on the fate of atrazine. However, since the protocols applied were designed to assess solutes, conclusions about the transport of atrazine loaded onto the nanocarriers should be made extremely cautiously. The centrifugation method applied over time (here over 7 days) appears to be a useful tool to indirectly assess the durability of nanopesticides under realistic soil-to-solution ratios and estimate the period of time during which an influence on the fate of the active ingredient may be expected. More detailed investigations into the bioavailability and durability of nanopesticides are necessary and will require the development of novel methods suitable to address both the "nano" and "organic" characteristics of polymer-based nanopesticides.

  18. Effect of cold storage on collagen-based hydrogels for the three-dimensional culture of adipose-derived stem cells

    International Nuclear Information System (INIS)

    Collagen gels have been extensively used as three-dimensional (3D) cell culture systems. To enhance their mechanical properties, the manufacture of collagen-based gels with agarose has been proposed. However, little is known about the stability of these gels under cold storage conditions. The consequences of cold storage on biological tissues for clinical applications are known to be significant; yet, they have not been considered on hydrogels used for in vitro experiments. This work studies the effect of extended cold storage on the stability of collagen and collagen-agarose hydrogels using rheometry and scanning electron microscopy. In addition, cell-matrix interactions of adipose-derived stem cells (ADSC) have been studied using these gels. Results show that both the storage modulus (G′) and loss modulus (G″) of pure collagen gels gradually decrease with extended cold storage along the 30 days of the study, while G′ and G″ increase in collagen-agarose gels under the same conditions. Moreover, significant changes in both moduli of collagen-agarose gels were only found after 30 days of cold storage, while in the case of collagen gels significant changes were already detected after 7 days. Finally, a reduction in the ability of ADSC to remodel the gel after prolonged cold storage was observed. To the best of our knowledge, this is the first work proving that cold storage of hydrogels prior to cell culture might have a significant impact on their mechanical properties and cell–matrix interactions. (paper)

  19. The Effect of Variation of Molarity of Alkali Activator and Fine Aggregate Content on the Compressive Strength of the Fly Ash: Palm Oil Fuel Ash Based Geopolymer Mortar

    Directory of Open Access Journals (Sweden)

    Iftekhair Ibnul Bashar

    2014-01-01

    Full Text Available The effect of molarity of alkali activator, manufactured sand (M-sand, and quarry dust (QD on the compressive strength of palm oil fuel ash (POFA and fly ash (FA based geopolymer mortar was investigated and reported. The variable investigated includes the quantities of replacement levels of M-sand, QD, and conventional mining sand (N-sand in two concentrated alkaline solutions; the contents of alkaline solution, water, POFA/FA ratio, and curing condition remained constant. The results show that an average of 76% of the 28-day compressive strength was found at the age of 3 days. The rate of strength development from 3 to 7 days was found between 12 and 16% and it was found much less beyond this period. The addition of 100% M-sand and QD shows insignificant strength reduction compared to mixtures with 100% N-sand. The particle angularity and texture of fine aggregates played a significant role in the strength development due to the filling and packing ability. The rough texture and surface of QD enables stronger bond between the paste and the fine aggregate. The concentration of alkaline solution increased the reaction rate and thus enhanced the development of early age strength. The use of M-sand and QD in the development of geopolymer concrete is recommended as the strength variation between these waste materials and conventional sand is not high.

  20. Effect of Ethanol on Fluoroquinolone Efficacy in a Rat Model of Pneumococcal Pneumonia

    Science.gov (United States)

    Olsen, Keith M.; Gentry-Nielsen, Martha; Yue, Mei; Snitily, Mary U.; Preheim, Laurel C.

    2006-01-01

    This investigation compared the effect of ethanol on fluoroquinolone antibiotic efficacy and pharmacodynamics in an ethanol-fed rat model of pneumococcal pneumonia. Male Sprague-Dawley rats received a liquid diet containing 36% of total calories as ethanol. Paired controls (pair-fed controls) were fed a liquid diet without ethanol or received rat chow. Diets began 7 days before and continued for 10 days after transtracheal infections with 10 times the 50% lethal dose of type 3 Streptococcus pneumoniae. Beginning 18 h after infection, the rats received once daily subcutaneous phosphate-buffered saline, levofloxacin, moxifloxacin, or trovafloxacin at 50 or 100 mg/kg of body weight. White blood cell counts were determined, blood samples were collected for culture, and mortality was recorded. Additional rats were killed on day 5 for pharmacodynamic studies and quantitative cultures of bronchoalveolar lavage fluid. Bacteremia occurred by day 3 in 20 of 22 untreated rats. All 22 untreated rats died by day 9. Moxifloxacin treatment was effective in all diet groups at both the 50- and 100-mg/kg doses. In contrast, 50-mg/kg doses of levofloxacin and trovafloxacin improved survival in ethanol-fed rats but were ineffective in chow-fed rats. High-dose trovafloxacin at 100 mg/kg was associated with increased mortality in pair-fed rats. The free-fraction area under the concentration-time curve/MIC ratio exceeded 50 with all antibiotics in the ethanol group but dropped below 30 with levofloxacin and trovafloxacin in the pair- and chow-fed rats, with higher mortality. Achievement of adequate antibiotic-free fraction area under the concentration-time curve/MIC ratios helps overcome ethanol-induced immune defects induced in experimental pneumococcal pneumonia. PMID:16377688

  1. Toxicodynamics of Rigid Polystyrene Microparticles on Pulmonary Gas Exchange in Mice: Implications for Microemboli-based Drug Delivery Systems

    Science.gov (United States)

    Kutscher, HL.; Gao, D.; Li, S.; Massa, CB.; Cervelli, J.; Deshmukh, M.; Joseph, LB.; Laskin, DL.; Sinko, PJ.

    2013-01-01

    The toxicodynamic relationship between the number and size of pulmonary microemboli resulting from uniformly sized, rigid polystyrene microparticles (MPs) administered intravenously and their potential effects on pulmonary gas exchange was investigated. CD-1 male mice (6–8 wk) were intravenously administered 10, 25 and 45 μm diameter MPs. Oxygen hemoglobin saturation in the blood (SpO2) was measured non-invasively using a pulse oximeter while varying inhaled oxygen concentration (FIO2). Resulting data were fit to a physiologically based non-linear mathematical model that estimates 2 parameters: ventilation-perfusion ratio (VA/Q) and shunt (percentage of deoxygenated blood returning to systemic circulation). The number of MPs administered prior to a statistically significant reduction in normalized VA/Q was dependent on particle size. MP doses that resulted in a significant reduction in normalized VA/Q one day post-treatment were 4,000, 40,000 and 550,000 MPs/g for 45, 25 and 10 μm MPs, respectively. The model estimated VA/Q and shunt returned to baseline levels 7 days post-treatment. Measuring SpO2 alone was not sufficient to observe changes in gas exchange; however, when combined with model-derived VA/Q and shunt early reversible toxicity from pulmonary microemboli was detected suggesting that the model and physical measurements are both required for assessing toxicity. Moreover, it appears that the MP load required to alter gas exchange in a mouse prior to lethality is significantly higher than the anticipated required MP dose for effective drug delivery. Overall, the current results indicate that the microemboli-based approach for targeted pulmonary drug delivery is potentially safe and should be further explored. PMID:23142466

  2. A new anti-tumor strategy based on in vivo tumstatin overexpression after plasmid electrotransfer in muscle

    International Nuclear Information System (INIS)

    Highlights: ► A new therapeutic strategy based on tumstatin in vivo overexpression is proposed. ► pVAX1©–tumstatin electrotransfer in muscle mediates protein expression in muscle. ► A substantial expression of tumstatin is detected in the serum of electrotransfected mice. ► Tumstatin overexpression decreases tumor growth and increases mouse survival. -- Abstract: The NC1 domains from the different α(IV) collagen chains were found to exert anti-tumorigenic and/or anti-angiogenic activities. A limitation to the therapeutic use of these matrikines is the large amount of purified recombinant proteins, in the milligram range in mice that should be administered daily throughout the experimental procedures. In the current study, we developed a new therapeutic approach based on tumstatin (NC1α3(IV)) overexpression in vivo in a mouse melanoma model. Gene electrotransfer of naked plasmid DNA (pDNA) is particularly attractive because of its simplicity, its lack of immune responsiveness and its safety. The pDNA electrotransfer in muscle mediates a substantial gene expression that lasts several months. A pVAX1© vector containing the tumstatin cDNA was injected into the legs of C57BL/6 mice and submitted to electrotranfer. Sera were collected at different times and tumstatin was quantified by ELISA. Tumstatin secretion reached a plateau at day 21 with an expression level of 12 μg/mL. For testing the effects of tumstatin expression on tumor growth in vivo, B16F1 melanoma cells were subcutaneously injected in mice 7 days after empty pVAX1© (Mock) or pVAX1©–tumstatin electrotransfer. Tumstatin expression triggered a large decrease in tumor growth and an increase in mouse survival. This new therapeutic approach seems promising to inhibit tumor progression in vivo

  3. Toxicodynamics of rigid polystyrene microparticles on pulmonary gas exchange in mice: implications for microemboli-based drug delivery systems.

    Science.gov (United States)

    Kutscher, H L; Gao, D; Li, S; Massa, C B; Cervelli, J; Deshmukh, M; Joseph, L B; Laskin, D L; Sinko, P J

    2013-01-15

    The toxicodynamic relationship between the number and size of pulmonary microemboli resulting from uniformly sized, rigid polystyrene microparticles (MPs) administered intravenously and their potential effects on pulmonary gas exchange were investigated. CD-1 male mice (6-8 weeks) were intravenously administered 10, 25 and 45 μm diameter MPs. Oxygen hemoglobin saturation in the blood (SpO(2)) was measured non-invasively using a pulse oximeter while varying inhaled oxygen concentration (F(I)O(2)). The resulting data were fit to a physiologically based non-linear mathematical model that estimates 2 parameters: ventilation-perfusion ratio (V(A)/Q) and shunt (percentage of deoxygenated blood returning to systemic circulation). The number of MPs administered prior to a statistically significant reduction in normalized V(A)/Q was dependent on particle size. MP doses that resulted in a significant reduction in normalized V(A)/Q one day post-treatment were 4000, 40,000 and 550,000 MPs/g for 45, 25 and 10 μm MPs, respectively. The model estimated V(A)/Q and shunt returned to baseline levels 7 days post-treatment. Measuring SpO(2) alone was not sufficient to observe changes in gas exchange; however, when combined with model-derived V(A)/Q and shunt early reversible toxicity from pulmonary microemboli was detected suggesting that the model and physical measurements are both required for assessing toxicity. Moreover, it appears that the MP load required to alter gas exchange in a mouse prior to lethality is significantly higher than the anticipated required MP dose for effective drug delivery. Overall, the current results indicate that the microemboli-based approach for targeted pulmonary drug delivery is potentially safe and should be further explored. PMID:23142466

  4. A novel study design for antibiotic trials in acute exacerbations of COPD: MAESTRAL methodology

    Directory of Open Access Journals (Sweden)

    Wilson R

    2011-06-01

    Full Text Available Robert Wilson1, Antonio Anzueto2, Marc Miravitlles3, Pierre Arvis4, Geneviève Faragó5, Daniel Haverstock6, Mila Trajanovic5, Sanjay Sethi71Host Defence Unit, Royal Brompton Hospital, London, England, UK; 2University of Texas Health Science Center at San Antonio, South Texas Veterans HealthCare System, San Antonio, TX, USA; 3Institut d'Investigacions Biomèdiques August Pi I Sunyer (IDIBAPS, Ciber de Enfermedades Respiratorias (CIBERES, Hospital Clinic, Barcelona, Spain; 4Bayer HealthCare, Loos, France; 5Bayer Inc, Toronto, ON, Canada; 6Bayer HealthCare Pharmaceuticals, Montville, NJ, USA; 7Division of Pulmonary, Critical Care and Sleep Medicine, University at Buffalo, State University of New York, Buffalo, NY, USAAbstract: Antibiotics, along with oral corticosteroids, are standard treatments for acute exacerbations of chronic obstructive pulmonary disease (AECOPD. The ultimate aims of treatment are to minimize the impact of the current exacerbation, and by ensuring complete resolution, reduce the risk of relapse. In the absence of superiority studies of antibiotics in AECOPD, evidence of the relative efficacy of different drugs is lacking, and so it is difficult for physicians to select the most effective antibiotic. This paper describes the protocol and rationale for MAESTRAL (moxifloxacin in AECBs [acute exacerbation of chronic bronchitis] trial; www.clinicaltrials.gov: NCT00656747, one of the first antibiotic comparator trials designed to show superiority of one antibiotic over another in AECOPD. It is a prospective, multinational, multicenter, randomized, double-blind controlled study of moxifloxacin (400 mg PO [per os] once daily for 5 days vs amoxicillin/clavulanic acid (875/125 mg PO twice daily for 7 days in outpatients with COPD and chronic bronchitis suffering from an exacerbation. MAESTRAL uses an innovative primary endpoint of clinical failure: the requirement for additional or alternate treatment for the exacerbation at 8 weeks

  5. Effects of quinolones on liver microsome cytochrome P450 in rats

    Directory of Open Access Journals (Sweden)

    Yi ZHANG

    2012-11-01

    Full Text Available Objective  To study and compare the effects of fluoroquinolones (levofloxacin, gatifloxacin, moxifloxacin and pazufloxacin on the enzyme system of liver microsome cytochrome P450 in rat. Methods  Thirty male Wistar rats were equally assigned into five groups: control group, levofloxacin (LV group, gatifloxacin (GT group, moxifloxacin (MX group and pazufloxacin (PZ group. Each drug was consecutively administered by tail vein injection for 7 days in a dosage of 120 mg/(kg•d. Liver microsomes were prepared by differential centrifugation, the concentration of protein in the liver microsome was measured by Lowry method, the content and activity of cy tochrome P450 were detected by spectrophotometric determination, and the results were analyzed by one-way ANOVA. Results  Compared with control group, the weight of liver in MX group and GT group was significantly reduced (P 0.05. Assay of aminopyrine-N-demethylase activity showed that the difference in enzyme activity was statistically significant between the control group and groups LV, GT and MX (P < 0.01. Erythromycin-N-demethylase activity measurement revealed that the enzyme activity was lowered in GT group and slightly elevated in MX group, and the difference was statistically significant compared with that of control group (P < 0.01. Measurement of activity of rat liver microsomal CYP450 enzyme system subfamily showed that the BROD activity increased in LV, MX and PZ groups (P < 0.01, and slightly decreased in GT group as compared with control group (P < 0.05. The PROD activity increased in GT group, but decreased in PZ group (P < 0.01. The EROD activity increased in all the four groups (P < 0.01. Conclusions  The four fluoroquinolones have some effects on the enzyme system of liver microsome cytochrome P450 in rats, but the effects may be different (enhancement or attenuation of the enzymatic activity depending on the enzymes, and the extent of the decrease of effect is in the

  6. A novel Listeria monocytogenes-based DNA delivery system for cancer gene therapy.

    LENUS (Irish Health Repository)

    van Pijkeren, Jan Peter

    2012-01-31

    Bacteria-mediated transfer of plasmid DNA to mammalian cells (bactofection) has been shown to have significant potential as an approach to express heterologous proteins in various cell types. This is achieved through entry of the entire bacterium into cells, followed by release of plasmid DNA. In a murine model, we show that Listeria monocytogenes can invade and spread in tumors, and establish the use of Listeria to deliver genes to tumors in vivo. A novel approach to vector lysis and release of plasmid DNA through antibiotic administration was developed. Ampicillin administration facilitated both plasmid transfer and safety control of vector. To further improve on the gene delivery system, we selected a Listeria monocytogenes derivative that is more sensitive to ampicillin, and less pathogenic than the wild-type strain. Incorporation of a eukaryotic-transcribed lysin cassette in the plasmid further increased bacterial lysis. Successful gene delivery of firefly luciferase to growing tumors in murine models and to patient breast tumor samples ex vivo was achieved. The model described encompasses a three-phase treatment regimen, involving (1) intratumoral administration of vector followed by a period of vector spread, (2) systemic ampicillin administration to induce vector lysis and plasmid transfer, and (3) systemic administration of combined moxifloxacin and ampicillin to eliminate systemic vector. For the first time, our results reveal the potential of Listeria monocytogenes for in vivo gene delivery.

  7. Plasma exchange-based plasma recycling dialysis system as a potential platform for artificial liver support.

    Science.gov (United States)

    Nishimura, Akimasa; Umehara, Yutaka; Umehara, Minoru; Hakamada, Kenichi; Narumi, Shunji; Toyoki, Yoshikazu; Yoshihara, Shuichi; Sasaki, Mutsuo

    2006-08-01

    We developed a plasma recycling dialysis (PRD) system based on plasma exchange (PE). In this system, rapid reduction of toxic substances and restitution of deficient essential substances are performed by PE, and subsequent blood purification is performed by dialysis between separated plasma recycled over a purification device and the patient's blood across the membrane of the plasma separator. This study was performed to demonstrate the safety and efficacy of this system. Hyperbilirubinemia was induced by ligating the bile duct in pigs, and 7 days later, only PE for 2 h (group PE) or PE for 2 h followed by PRD for 6 h (group PE + PRD) was performed. The separated plasma was recycled over anion-exchange resin through the extra fiber space of the plasma separator. The safety and efficacy of this system were evaluated based on the values of hemodynamic and laboratory parameters. Transfer from PE to PRD was completed in a few minutes. The hemodynamic status and blood cells counts were stable and hemolysis was not observed during the procedure. In the PE + PRD group, the concentrations of total bile acids continuously decreased (pretreatment, 155.5 +/- 40.6 microM; 2 h [end of PE], 76.1 +/- 14.4 microM; 8 h [end of PRD], 25.8 +/- 9.1 microM) and the value was significantly lower than in the PE group after 6 h. The total bilirubin also continuously decreased during PRD (pretreatment, 55.3 +/- 11.5 microM; 2 h [end of PE], 33.8 +/- 8.4 microM; 8 h [end of PRD], 18.6 +/- 7.7 microM) and was significantly lower than in the PE group after 4 h. No significant change was observed in other laboratory values. This PE-based PRD system allowed a swift transfer from PE to sorbent-based blood purification. The safety of this system was demonstrated and the removal of toxic substances was significant. This study confirmed the clinical utility of this system as a platform for artificial liver support. PMID:16911318

  8. Connectomic and surface-based morphometric correlates of acute mild traumatic brain injury

    Directory of Open Access Journals (Sweden)

    Patrizia eDall'Acqua

    2016-03-01

    Full Text Available Reduced integrity of white matter (WM pathways and subtle anomalies in gray matter (GM morphology have been hypothesized as mechanisms in mild traumatic brain injury (mTBI. However, findings on structural brain changes in early stages after mTBI are inconsistent and findings related to early symptoms severity are rare.Fifty-one patients were assessed with multimodal neuroimaging and clinical methods exclusively within 7 days following mTBI and compared to 53 controls. Whole-brain connectivity based on diffusion tensor imaging was subjected to network-based statistics, whereas cortical surface area, thickness, and volume based on T1-weighted MRI scans were investigated using surface-based morphometric analysis. Reduced connectivity strength within a subnetwork of 59 edges located predominantly in bilateral frontal lobes was significantly associated with higher levels of self-reported symptoms. In addition, cortical surface area decreases were associated with stronger complaints in five clusters located in bilateral frontal and postcentral cortices, and in the right inferior temporal region. Alterations in WM and GM were localized in similar brain regions and moderately-to-strongly related to each other. Furthermore, the reduction of cortical surface area in the frontal regions was correlated with poorer attentive-executive performance in the mTBI group. Finally, group differences were detected in both the WM and GM, especially when focusing on a subgroup of patients with greater complaints, indicating the importance of classifying mTBI patients according to severity of symptoms. This study provides evidence that mTBI affects not only the integrity of WM networks by means of axonal damage but also the morphology of the cortex during the initial post-injury period. These anomalies might be greater in the acute period than previously believed and the involvement of frontal brain regions was consistently pronounced in both findings. The dysconnected

  9. Determining the Feasibility of Ambulance-Based Randomised Controlled Trials in Patients with Ultra-Acute Stroke: Study Protocol for the "Rapid Intervention with GTN in Hypertensive Stroke Trial" (RIGHT, ISRCTN66434824).

    Science.gov (United States)

    Ankolekar, Sandeep; Sare, Gillian; Geeganage, Chamila; Fuller, Michael; Stokes, Lynn; Sprigg, Nikola; Parry, Ruth; Siriwardena, A Niroshan; Bath, Philip M W

    2012-01-01

    Background. Time from acute stroke to enrolment in clinical trials needs to be reduced to improve the chances of finding effective treatments. No completed randomised controlled trials of ambulance-based treatment for acute stroke have been reported in the UK, and the practicalities of recruiting, consenting, and treating patients are unknown. Methods. RIGHT is an ambulance based, single-blind, randomised controlled trial with blinded-outcome assessment. The trial will assess feasibility of using ambulance services to deliver ultra-acute stroke treatments; a secondary aim is to assess the effect of glyceryl trinitrate (GTN) on haemodynamic variables and functional outcomes. Initial consent, randomisation, and treatment are performed by paramedics prior to hospitalisation. Patients with ultra-acute stroke (≤4 hours of onset) are randomised to transdermal GTN (5 mg/24 hours) or gauze dressing daily for 7 days. The primary outcome is systolic blood pressure at 2 hours. Secondary outcomes include feasibility, haemodynamics, dependency, and other functional outcomes. A nested qualitative study is included. Trial Status. The trial has all relevant ethics and regulatory approvals and recruitment started on February 15, 2010. The trial stopped recruitment in December 2011 after 41 patients were recruited. Trial Registration. The trial registration number is ISRCTN66434824 and EudraCT number is 2007-004766-40.

  10. A dynamic knowledge base based search engine

    Institute of Scientific and Technical Information of China (English)

    WANG Hui-jin; HU Hua; LI Qing

    2005-01-01

    Search engines have greatly helped us to find thedesired information from the Intemet. Most search engines use keywords matching technique. This paper discusses a Dynamic Knowledge Base based Search Engine (DKBSE), which can expand the user's query using the keywords' concept or meaning. To do this, the DKBSE needs to construct and maintain the knowledge base dynamically via the system's searching results and the user's feedback information. The DKBSE expands the user's initial query using the knowledge base, and returns the searched information after the expanded query.

  11. Knowledge Based Strategies for Knowledge Based Organizations

    Directory of Open Access Journals (Sweden)

    Madalina Cristina Tocan

    2012-12-01

    Full Text Available In the present, we can observe that a new economy is arising. It is an economy based on knowledge and ideas, in which the key factor for prosperity and for creation of the new jobs is the knowledge capitalization. Knowledge capitalization, intellectual capital, obtaining prosperity in the market economy imposes a new terminology, new managerial methods and techniques, new technologies and also new strategies. In other words, knowledge based economy, as a new type of economy; impose a new type of management- knowledge based management and a new type of enterprise-knowledge based enterprise.Taking in consideration these predictable evolutions, this paper will try to present: • The main ideas of the researches the field of the knowledge based economy • The characteristics and principles of the knowledge based organizations and knowledge based management • The challenges and opportunities for knowledge based organizations.The first part of the paper will present the principles of the new economy- knowledge base economy, the essential and interrelated elements necessary to build and to implement a strategy in a knowledge economy and the opportunities and challenges to the countries as they seek to build regionally and globally competitive knowledge-based economies.The second part of the paper will present the characteristics of the knowledge based organizations, the principles and characteristics of their management. For many organizations, knowledge management is a relatively new concept, because they are discovering the knowledge value in their operations. Many firms have undertaken formal and informal knowledge management initiatives designed to improve process performance, increase customer responsiveness and spur innovation. But while some organizations have reaped significant benefits from their investment in knowledge efforts, others have run into noteworthy challenges.

  12. Relationship of heat treatment-mechanical properties of nickel base superalloys

    International Nuclear Information System (INIS)

    The nickel-base superalloys have high strength, excellent corrosion resistant, and good creep and fatigue resistance. These alloy improved properties at high temperature derive their mechanical and creep behavior on γ precipitate morphology, and the evolution of such morphology during different heat treatment conditions. The main microstructural variable of Nickel-based superalloys, responsible for the mechanical properties are: a) amount and morphology of precipitates; b) size and shape of grains; and c) carbide distribution. In this work, a Nickel-base superalloy Nimonic 80A, modified little with Zr prepared by melting and casting practices of materials electrolytic in vacuum-induction melting (VIM) type Balzers, to obtain five alloys different and ingots of 2 Kg and 1 Kg, with composition in weight % of Nimonic 80-A is: Ni = bal (76.66), C = 0.01, Cr = 19.83, Fe = 2.4, Mn = 0.17, Si 0.47, Al = 0.19, Zr = 0.4. The solidification process is made in a steel mold. After having realized four thermal treatments, the most representative microstructures there were obtained. The results from tensile tests performed on Instron Servohydraulic testing systems at uniaxial dynamic testing, at constant speeds to ,0.2 cm/min, were: the yield strength, the ultimate strength value, percentage elongation and area reduction. Creep tests were performed at in stress of 90 and 129 MPa, at a temperature of 600 and 680 Centigrades at different times and width of specimen of 1 mm. The alloys were analyzed by MEB(JEOL 35CF) at different magnifications. The nucleation and growth of intergranular cavities during creep of alloy Nimonic M3, were investigated. One sample was deformed in creep at 129 MPa and 680 Centigrades during 110 hs. Creep samples were annealing heat treated at 800 Centigrades, during 7 days. After a careful sample preparation procedure, 3100 of cavities were measured in the sample . The cavity size distributions in the sample were obtained. The cavity growth rate, was

  13. Winner of the Young Investigator Award of the Society for Biomaterials at the 10th World Biomaterials Congress, May 17-22, 2016, Montreal QC, Canada: Microribbon-based hydrogels accelerate stem cell-based bone regeneration in a mouse critical-size cranial defect model.

    Science.gov (United States)

    Han, Li-Hsin; Conrad, Bogdan; Chung, Michael T; Deveza, Lorenzo; Jiang, Xinyi; Wang, Andrew; Butte, Manish J; Longaker, Michael T; Wan, Derrick; Yang, Fan

    2016-06-01

    Stem cell-based therapies hold great promise for enhancing tissue regeneration. However, the majority of cells die shortly after transplantation, which greatly diminishes the efficacy of stem cell-based therapies. Poor cell engraftment and survival remain a major bottleneck to fully exploiting the power of stem cells for regenerative medicine. Biomaterials such as hydrogels can serve as artificial matrices to protect cells during delivery and guide desirable cell fates. However, conventional hydrogels often lack macroporosity, which restricts cell proliferation and delays matrix deposition. Here we report the use of injectable, macroporous microribbon (μRB) hydrogels as stem cell carriers for bone repair, which supports direct cell encapsulation into a macroporous scaffold with rapid spreading. When transplanted in a critical-sized, mouse cranial defect model, μRB-based hydrogels significantly enhanced the survival of transplanted adipose-derived stromal cells (ADSCs) (81%) and enabled up to three-fold cell proliferation after 7 days. In contrast, conventional hydrogels only led to 27% cell survival, which continued to decrease over time. MicroCT imaging showed μRBs enhanced and accelerated mineralized bone repair compared to hydrogels (61% vs. 34% by week 6), and stem cells were required for bone repair to occur. These results suggest that paracrine signaling of transplanted stem cells are responsible for the observed bone repair, and enhancing cell survival and proliferation using μRBs further promoted the paracrine-signaling effects of ADSCs for stimulating endogenous bone repair. We envision μRB-based scaffolds can be broadly useful as a novel scaffold for enhancing stem cell survival and regeneration of other tissue types. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1321-1331, 2016.

  14. Empirical Comparison on Risk-free Interest Rate Based on Three Benchmarks%三种度量基准下的无风险利率实证比较

    Institute of Scientific and Technical Information of China (English)

    秦学志; 胡友群; 张康

    2011-01-01

    Taking Shanghai Composite Index, Shenzhen Component Index and Shenzhen-Shanghai 300 Index as the samples, this paper constructs a multi-factor model. And based on the monthly data about index return and factors, it studies empirically implied risk-free interest rates and risk values of Shanghai stock market,Shenzhen stock market and the national comprehensive stock market by using the least square method. The results show as follows;the implied risk compensation in stock market is negative, which is contrary to the traditional theory of risk premium;the implied risk-free interest rate is quite different from short-term bank deposit rate, 7-day Shibor rate or 7-day bond repurchase rate. Therefore,these indicators should not be unselectively used as the proxy indicator of risk-free interest rate when studying financial derivative.%以上证综指、深圳成指和沪深300指数为研究样本,构建了多因子模型,并利用2003年1月-2009年2月三类指数收益率及各因子的月度数据,用最小二乘法实证反演了上海证券交易市场、深圳证券交易市场以及沪深综合证券交易市场隐含的无风险利率和风险价值。研究发现:股市隐含的风险补偿为负,与传统的风险溢价理论相悖;以短期银行存款利率、7天Shibor利率及7天国债回购利率为度量基准,股市隐含的无风险利率与其存在较大差异,因此在金融衍生品等相关研究中不宜不加选择地将它们作为无风险利率的代理指标。

  15. Dendritic cell-based vaccines in the setting of peripheral blood stem cell transplantation: CD34+ cell-depleted mobilized peripheral blood can serve as a source of potent dendritic cells.

    Science.gov (United States)

    Choi, D; Perrin, M; Hoffmann, S; Chang, A E; Ratanatharathorn, V; Uberti, J; McDonagh, K T; Mulé, J J

    1998-11-01

    We are investigating the use of tumor-pulsed dendritic cell (DC)-based vaccines in the treatment of patients with advanced cancer. In the current study, we evaluated the feasibility of obtaining both CD34+ hematopoietic stem/ progenitor cells (HSCs) and functional DCs from the same leukapheresis collection in adequate numbers for both peripheral blood stem cell transplantation (PBSCT) and immunization purposes, respectively. Leukapheresis collections of mobilized peripheral blood mononuclear cells (PBMCs) were obtained from normal donors receiving granulocyte colony-stimulating factor (G-CSF) (for allogeneic PBSCT) and from intermediate grade non-Hodgkin's lymphoma or multiple myeloma patients receiving cyclophosphamide plus G-CSF (for autologous PBSCT). High enrichment of CD34+ HSCs was obtained using an immunomagnetic bead cell separation device. After separation, the negative fraction of mobilized PBMCs from normal donors and cancer patients contained undetectable levels of CD34+ HSCs by flow cytometry. This fraction of cells was then subjected to plastic adherence, and the adherent cells were cultured for 7 days in GM-CSF (100 ng/ml) and interleukin 4 (50 ng/ml) followed by an additional 7 days in GM-CSF, interleukin 4, and tumor necrosis factor alpha (10 ng/ml) to generate DCs. Harvested DCs represented yields of 4.1+/-1.4 and 5.8+/-5.4% of the initial cells plated from the CD34+ cell-depleted mobilized PBMCs of normal donors and cancer patients, respectively, and displayed a high level expression of CD80, CD86, HLA-DR, and CD11c but not CD14. This phenotypic profile was similar to that of DCs derived from non-CD34+ cell-depleted mobilized PBMCs. DCs generated from CD34+ cell-depleted mobilized PBMCs elicited potent antitetanus as well as primary allogeneic T-cell proliferative responses in vitro, which were equivalent to DCs derived from non-CD34+ cell-depleted mobilized PBMCs. Collectively, these results demonstrate the feasibility of obtaining both DCs and

  16. Solid Base Catalysis

    CERN Document Server

    Ono, Yoshio

    2011-01-01

    The importance of solid base catalysts has come to be recognized for their environmentally benign qualities, and much significant progress has been made over the past two decades in catalytic materials and solid base-catalyzed reactions. The book is focused on the solid base. Because of the advantages over liquid bases, the use of solid base catalysts in organic synthesis is expanding. Solid bases are easier to dispose than liquid bases, separation and recovery of products, catalysts and solvents are less difficult, and they are non-corrosive. Furthermore, base-catalyzed reactions can be performed without using solvents and even in the gas phase, opening up more possibilities for discovering novel reaction systems. Using numerous examples, the present volume describes the remarkable role solid base catalysis can play, given the ever increasing worldwide importance of "green" chemistry. The reader will obtain an overall view of solid base catalysis and gain insight into the versatility of the reactions to whic...

  17. A phase Ⅱ clinical trial of celecoxib combined with platinum-based chemotherapy in the treatment of patients with advanced NSCLC as first-line treatment

    Directory of Open Access Journals (Sweden)

    Zhijie WANG

    2008-06-01

    Full Text Available Background and objective Currently, platinum-based regimes are standard first-line chemotherapy for non-small cell lung cancer. The aim of this study is to evaluate the efficacy, influencing factors and adverse events of celecoxib plus platinum-based chemotherapy in advanced non-small cell lung cancer as first-line treatment. Methods Previously untreated patients of advanced non-small cell lung cancer with COX-2 positive, confirmed by immunohistochemical staining, were randomized to receive GP, NP or TP regimens. Celecoxib of 400 mg Bid was given, po 5-7 days before chemotherapy,and not stopped until evidence of disease progression or toxicity. Adverse events were cirtified according to NCI-CTC criteria. Kaplan-Meier method was used to analyze the survival rate. COX regression was used to define the predictive factors. Primary endpoint: median survival time, 1-year survival rate and median progression free survival time. Secondary endpoint: response rate and toxicity. Results Forty-four evaluable patients were enrolled in the study from February 2005 to March 2007. Response rate was 45%, disease control rate 59%. Median progression free survival time and median survival time were 6months (95%CI: 4-8 months and 18 months (95%CI: 9-27months, respectively. One-year survival rate was 68%. Numbers of chemotherapy cycles and overall evaluation were the predictive factors for PFS in COX model (P=0.023 and 0.000. No factor was defined to affect survival time. Neutropenia and nausea/vomit were the most common toxicity. Conclusion Celecoxib combined with platinum-based chemotherapy appears to be well tolerated and demonstrates encouraging activity in patients of previously untreated advanced NSCLC.

  18. Comparison of passive transfer of immunity in neonatal dairy calves fed colostrum or bovine serum-based colostrum replacement and colostrum supplement products

    Science.gov (United States)

    Poulsen, Keith P.; Foley, Andrea L.; Collins, Michael T.; McGuirk, Sheila M.

    2011-01-01

    Objective To compare serum total protein (sTP) and serum IgG (sIgG) concentrations in neonatal calves administered colostrum or a bovine serum-based colostrum replacement (CR) product followed by a bovine serum-based colostrum supplement (CS) product. Design Randomized controlled clinical trial. Animals 18 Jersey and 269 Holstein neonatal heifer calves. Procedures 141 calves were given 4 L of colostrum in 1 or 2 feedings (first or only feeding was provided ≤ 2 hours after birth; when applicable, a second feeding was provided between 2 and 12 hours after birth). Other calves (n = 146) were fed 2 L of a CR product ≤ 2 hours after birth and then 2 L of a CS product between 2 and 12 hours after birth. Concentrations of sTP and sIgG were measured 1 to 7 days after birth. Data from cohorts on individual farms and for all farms were analyzed. Results Mean sTP and sIgG concentrations differed significantly between feeding groups. In calves fed colostrum and calves fed CR and CS products, mean ± SD sTP concentration was 5.58 ± 0.67 g/dL and 5.26 ± 0.54 g/dL, respectively, and mean sIgG concentration was 1,868 ± 854 mg/dL and 1,320 ± 620 mg/dL, respectively. The percentage of calves that had failure of passive transfer of immunity (ie, sIgG concentrations < 1,000 mg/dL) was not significantly different between groups. Conclusions and Clinical Relevance Results suggested that sequential feeding of bovine serum-based CR and CS products to neonatal calves is an alternative to feeding colostrum for achieving passive transfer of immunity. PMID:20946083

  19. VectorBase

    Data.gov (United States)

    U.S. Department of Health & Human Services — VectorBase is a Bioinformatics Resource Center for invertebrate vectors. It is one of four Bioinformatics Resource Centers funded by NIAID to provide web-based...

  20. Mobile Inquiry Based Learning

    NARCIS (Netherlands)

    Specht, Marcus

    2012-01-01

    Specht, M. (2012, 8 November). Mobile Inquiry Based Learning. Presentation given at the Workshop "Mobile inquiry-based learning" at the Mobile Learning Day 2012 at the Fernuniversität Hagen, Hagen, Germany.

  1. The ground based plan

    International Nuclear Information System (INIS)

    The paper presents a report of ''The Ground Based Plan'' of the United Kingdom Science and Engineering Research Council. The ground based plan is a plan for research in astronomy and planetary science by ground based techniques. The contents of the report contains a description of:- the scientific objectives and technical requirements (the basis for the Plan), the present organisation and funding for the ground based programme, the Plan, the main scientific features and the further objectives of the Plan. (U.K.)

  2. "Education-based Research"

    DEFF Research Database (Denmark)

    Degn Johansson, Troels

    This paper lays out a concept of education-based research-the production of research knowledge within the framework of tertiary design education-as an integration of problem-based learning and research-based education. This leads to a critique of reflective practice as the primary way to facilitate...... learning at this level, a discussion of the nature of design problems in the instrumentalist tradition, and some suggestions as to how design studies curricula may facilitate education-based research....

  3. Stolen Base Physics

    Science.gov (United States)

    Kagan, David

    2013-01-01

    Few plays in baseball are as consistently close and exciting as the stolen base. While there are several studies of sprinting, the art of base stealing is much more nuanced. This article describes the motion of the base-stealing runner using a very basic kinematic model. The model will be compared to some data from a Major League game. The…

  4. LC/MS-based metabolomics strategy to assess the amelioration effects of ginseng total saponins on memory deficiency induced by simulated microgravity.

    Science.gov (United States)

    Feng, Li; Yue, Xiao-Fei; Chen, Yi-Xi; Liu, Xin-Min; Wang, Li-Sha; Cao, Fang-Rui; Wang, Qiong; Liao, Yong-Hong; Pan, Rui-le; Chang, Qi

    2016-06-01

    Microgravity-induced memory deficiency seriously affects learning and memory ability of the astronaut during spaceflight, with few effective countermeasures. Panax ginseng C. A. Mey. has been used as a nootropic herb for thousands of years in Asian countries. Saponins are recognized as its major active components. Previous studies have shown that ginseng saponins offer protection against memory deficits caused by various factors. Nevertheless, the underlying mechanisms of their nootropic effects are still largely unknown. In this study, we evaluated the memory-improving effects of ginseng total saponins (GTS) on simulated microgravity hindlimb-unloaded rats using a metabolomics approach. After being exposed to a 7-days hindlimb unloading (HU), variations of plasmatic and hippocampal metabolic profiles of rats with and without GTS intervention were examined by a liquid chromatography-mass spectrometry (LC-MS) based untargeted metabolomics method. Subsequently, 8 hippocampal neurotransmitters were determined using a LC-MS/MS method. Finally, a LC-MS/MS based targeted metabolomics was performed to validate biomarkers found in the untargeted analysis. Besides, to support the metabolomics results, passive avoidance (PA) test, Nissl staining, and plasmatic corticosterone (CORT) levels determination were performed. The results showed that HU could lead to variations of 7 neurotransmitters and significantly different plasmatic and hippocampal metabolic profiles. GTS could restore most of the imbalanced neurotransmitters, especially glutamic acid and acetylcholine, and correct the levels of various disturbed learning and memory relevant biomarkers such as asparagine, phenylalanine, tyrosine, tryptophan, and choline. In addition, GTS could markedly ameliorate HU-induced memory deficiency, protect hippocampal neurons from damage, and down-regulate elevated CORT levels. In conclusion, GTS exhibits memory-improving effects mainly through regulating the metabolism of amino acids

  5. Clearance of Vancomycin-Resistant Enterococcus Concomitant With Administration of a Microbiota-Based Drug Targeted at Recurrent Clostridium difficile Infection

    Science.gov (United States)

    Dubberke, Erik R.; Mullane, Kathleen M.; Gerding, Dale N.; Lee, Christine H.; Louie, Thomas J.; Guthertz, Harriet; Jones, Courtney

    2016-01-01

    Background. Vancomycin-resistant Enterococcus (VRE) is a major healthcare-associated pathogen and a well known complication among transplant and immunocompromised patients. We report on stool VRE clearance in a post hoc analysis of the Phase 2 PUNCH CD study assessing a microbiota-based drug for recurrent Clostridium difficile infection (CDI). Methods. A total of 34 patients enrolled in the PUNCH CD study received 1 or 2 doses of RBX2660 (microbiota suspension). Patients were requested to voluntarily submit stool samples at baseline and at 7, 30, and 60 days and 6 months after the last administration of RBX2660. Stool samples were tested for VRE using bile esculin azide agar with 6 µg/mL vancomycin and Gram staining. Vancomycin resistance was confirmed by Etest. Results. VRE status (at least 1 test result) was available for 30 patients. All stool samples for 19 patients (63.3%, mean age 61.7 years, 68% female) tested VRE negative. Eleven patients (36.7%, mean age 75.5 years, 64% female) were VRE positive at the first test (baseline or 7-day follow-up). Of these patients, 72.7%, n = 8 converted to negative as of the last available follow-up (30 or 60 days or 6 months). Of the other 3: 1 died (follow-up data not available); 1 patient remained positive at all follow-ups; 1 patient retested positive at 6 months with negative tests during the interim. Conclusions. Although based on a small sample size, this secondary analysis demonstrated the possibility of successfully converting a high percentage of VRE-positive patients to negative in a recurrent CDI population with RBX2660.

  6. Expression of cumulus-oocyte complex genes and embryonic development in goats subjected to progestogen-based estrus synchronization.

    Science.gov (United States)

    Thammasiri, Jiratti; Navanukraw, Chainarong; Uriyapongson, Suthipong; Khanthusaeng, Vilaivan; Kamollirt, Chuchat

    2016-07-15

    The objective was to investigate the effect of short-term (7 days) and long-term (14 days) progesterone-based estrus synchronization on number of follicles, progesterone concentrations, cumulus-oocyte complex (COC) gene expression, and embryonic development in goats. Nulliparous Thai-native goats (n = 45) were randomly assigned to one of two estrus synchronization treatments. Goats were treated with intravaginal sponges containing 60-mg medroxyprogesterone acetate (MAP; Synchrogest esponjas, Spain) during 7 or 14 days (short-term or long-term protocol, respectively). Multiple follicular development was induced by intramuscularly injections of 300-IU eCG in both groups (1 day before sponge withdrawal). An ovariectomy was performed at 24 hours after sponge removal to evaluate number of follicle and collect oocyte for IVF. Oocyte quality (healthy or nonhealthy) was determined by morphology of COCs before IVM. Recovery of COCs and total cellular RNA isolation were applied to determine apoptosis-related gene expression. After IVF, embryos were evaluated during the eight-day culture as numbers of cleaved oocyte, morula, and blastocyst embryo. Total numbers of follicles and oocytes were similar for both treatments. Plasma progesterone concentrations were not different during MAP insertion period (P > 0.05). However, goats that received the short-term protocol had a greater number of 4 to 6-mm follicle, healthy oocytes, cleaved oocytes, and morula embryos than goats that received the long-term protocol (P < 0.01). In addition, the expression of B-cell lymphoma 2 messenger RNA was greater (P < 0.05) in COCs derived from the 7 days MAP-treated when compared to the 14 days MAP-treated goats. These data highlight that the 7-day progestin-based treatment may contribute to quality of oocytes and embryonic development in goats. PMID:27056418

  7. The Survey of Birth Defects Rate Based on Birth Registration System

    OpenAIRE

    Min Yu; Zhiguang Ping; Shuiping Zhang; Yuying He; Rui Dong; Xiong Guo

    2015-01-01

    Background: To investigate the surveillance trend of birth defects, incidence, distribution, occurrence regularity, and their relevant factors in Xi′an City in the last 10 years for proposing control measures. Methods: The birth defects monitoring data of infants during perinatal period (28 weeks of gestation to 7 days after birth) were collected from obstetrics departments of all hospitals during 2003-2012. Microsoft Excel 2003 was used for data input, and Statistical Package for the Soc...

  8. The Survey of Birth Defects Rate Based on Birth Registration System

    OpenAIRE

    Yu, Min; Ping, Zhiguang; Zhang, Shuiping; He, Yuying; Dong, Rui; Guo, Xiong

    2015-01-01

    Background: To investigate the surveillance trend of birth defects, incidence, distribution, occurrence regularity, and their relevant factors in Xi’an City in the last 10 years for proposing control measures. Methods: The birth defects monitoring data of infants during perinatal period (28 weeks of gestation to 7 days after birth) were collected from obstetrics departments of all hospitals during 2003–2012. Microsoft Excel 2003 was used for data input, and Statistical Package for the Social ...

  9. Outcomes of a Clinic-Based Pediatric Constraint-Induced Movement Therapy Program

    Science.gov (United States)

    Reidy, Teressa Garcia; Naber, Erin; Viguers, Emily; Allison, Kristen; Brady, Kathleen; Carney, Joan; Salorio, Cynthia; Pidcock, Frank

    2012-01-01

    A single-group pre- and post-test design was used to evaluate functional outcomes of a constraint-induced movement therapy (CIMT) protocol implemented in an outpatient therapy center. The participants were 29 children with hemiplegia, ages 1.6-19.1 years old. The less-involved upper limb was placed in a cast that was worn 24 hr a day, 7 days a…

  10. Contribution of the Nitroimidazoles PA-824 and TBA-354 to the Activity of Novel Regimens in Murine Models of Tuberculosis

    OpenAIRE

    Tasneen, Rokeya; Williams, Kathy; Amoabeng, Opokua; Minkowski, Austin; Mdluli, Khisimuzi E.; Upton, Anna M.; Eric L Nuermberger

    2014-01-01

    New regimens based on two or more novel agents are sought in order to shorten or simplify the treatment of both drug-susceptible and drug-resistant forms of tuberculosis. PA-824 is a nitroimidazo-oxazine now in phase II trials and has shown significant early bactericidal activity alone and in combination with the newly approved agent bedaquiline or with pyrazinamide with or without moxifloxacin. While the development of PA-824 continues, a potential next-generation derivative, TBA-354, has be...

  11. Designing tragacanth gum based sterile hydrogel by radiation method for use in drug delivery and wound dressing applications.

    Science.gov (United States)

    Singh, Baljit; Varshney, Lalit; Francis, Sanju; Rajneesh

    2016-07-01

    Present article discusses synthesis and characterization of the sterile and pure hydrogel wound dressings which were prepared through radiation method by using polyvinyl alcohol (PVA), tragacanth gum (TG) and sodium alginate (SA). The polymer films were characterized by SEM, Cryo-SEM, FTIR, solid state C(13) NMR and XRD, TGA, and DSC. Some important biological properties such as O2 permeability, water vapor transmission rate, microbial permeability, haemolysis, thrombogenic behavior, antioxidant activity, bio-adhesion and mechanical properties were also studied. The hydrogel film showed thrombogenicity (82.43±1.54%), haemolysis (0.83±0.09%), oxygen permeability (6.433±0.058mg/L) and water vapor permeability (197.39±25.34g/m(2)/day). Hydrogel films were found biocompatible and impermeable to microbes. The release of antibiotic drug moxifloxacin occurred through non-Fickian mechanism and release profile was best fitted in Hixson-Crowell model for drug release. Overall, these results indicate the suitability of these hydrogels in wound dressing applications. PMID:27020943

  12. Web-based smoking cessation intervention that transitions from inpatient to outpatient: study protocol for a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Harrington Kathleen F

    2012-08-01

    Full Text Available Abstract Background E-health tools are a new mechanism to expand patient care, allowing supplemental resources to usual care, including enhanced patient-provider communication. These applications to smoking cessation have yet to be tested in a hospitalized patient sample. This project aims to evaluate the effectiveness and cost-effectiveness of a tailored web-based and e-message smoking cessation program for current smokers that, upon hospital discharge, transitions the patient to continue a quit attempt when home (Decide2Quit. Design A randomized two-arm follow-up design will test the effectiveness of an evidence- and theoretically-based smoking cessation program designed for post-hospitalization. Methods A total of 1,488 patients aged 19 or older, who smoked cigarettes in the previous 30 days, are being recruited from 27 patient care areas of a large urban university hospital. Study-eligible hospitalized patients receiving usual tobacco cessation usual care are offered study referral. Trained hospital staff assist the 744 patients who are being randomized to the intervention arm with registration and orientation to the intervention website. This e-mail and web-based program offers tailored messages as well as education, self-assessment and planning aids, and social support to promote tobacco use cessation. Condition-blind study staff assess participants for tobacco use history and behaviors, tobacco use cost-related information, co-morbidities and psychosocial factors at 0, 3, 6, and 12 months. The primary outcome is self-reported 30-day tobacco abstinence at 6 months follow-up. Secondary outcomes include 7-day point prevalence quit rates at 3-, 6-, and 12-month follow-up, 30-day point prevalence quit rates at 3 and 12 months, biologically confirmed tobacco abstinence at 6-month follow-up, and multiple point-prevalence quit rates based on self-reported tobacco abstinence rates at each follow-up time period. Healthcare utilization and quality

  13. Impact of 4.0% chlorhexidine cleansing of the umbilical cord on mortality and omphalitis among newborns of Sylhet, Bangladesh: design of a community-based cluster randomized trial

    Directory of Open Access Journals (Sweden)

    Ahmed Saifuddin

    2009-10-01

    Full Text Available Abstract Background The World Health Organization recommends dry cord care for newborns but this recommendation may not be optimal in low resource settings where most births take place in an unclean environment and infections account for up to half of neonatal deaths. A previous trial in Nepal indicated that umbilical cord cleansing with 4.0% chlorhexidine could substantially reduce mortality and omphalitis risk, but policy changes await additional community-based data. Methods The Projahnmo Chlorhexidine study was a three-year, cluster-randomized, community-based trial to assess the impact of three cord care regimens on neonatal mortality and omphalitis. Women were recruited mid-pregnancy, received a basic package of maternal and neonatal health promotion messages, and were followed to pregnancy outcome. Newborns were visited at home by local village-based workers whose areas were randomized to either 1 single- or 2 7-day cord cleansing with 4.0% chlorhexidine, or 3 promotion of dry cord care as recommended by WHO. All mothers received basic messages regarding hand-washing, clean cord cutting, and avoidance of harmful home-base applications to the cord. Death within 28 days and omphalitis were the primary outcomes; these were monitored directly through home visits by community health workers on days 1, 3, 6, 9, 15, and 28 after birth. Discussion Due to report in early 2010, the Projahnmo Chlorhexidine Study examines the impact of multiple or single chlorhexidine cleansing of the cord on neonatal mortality and omphalitis among newborns of rural Sylhet District, Bangladesh. The results of this trial will be interpreted in conjunction with a similarly designed trial previously conducted in Nepal, and will have implications for policy guidelines for optimal cord care of newborns in low resource settings in Asia. Trial Registration ClinicalTrials.gov (NCT00434408

  14. Alteração dimensional de amálgama de prata e liga à base de gálio Dimensional alteration of silver amalgam and gallium-based alloy

    Directory of Open Access Journals (Sweden)

    Rafael Yagüe BALLESTER

    2001-12-01

    -setting expansion, and the latter is capable of cracking dental structures. The aim of this study was to evaluate, during 7 days, the in vitro dimensional alteration of a gallium dental alloy (Galloy, SDI, Australia, in comparison with a dental amalgam containing zinc (F400, SDI, Australia, as a function of the contact with saline solution (0.9% NaCl during the setting period. The storage experimental conditions were: storage in dry environment, immersion in saline solution and contamination during condensation. Additionally, the effects of contamination during the trituration of dental amalgam and the effects of protecting the surface of the gallium alloy with a fluid resin were studied. Specimens were stored at 37ºC ± 1ºC, and measuring was carried out, sequentially, every 24 h during 7 days. When the gallium alloy was either contaminated or immersed, an expansion significantly greater than that observed in the other experimental conditions was noticed after 7 days. The application of a fluid resin to protect the surface of the cylinders was able to avoid the increase in expansion caused by superficial moisture. The amalgam alloy did not show significant dimensional alterations, except when it was contaminated during trituration.

  15. Case-based reasoning

    CERN Document Server

    Kolodner, Janet

    1993-01-01

    Case-based reasoning is one of the fastest growing areas in the field of knowledge-based systems and this book, authored by a leader in the field, is the first comprehensive text on the subject. Case-based reasoning systems are systems that store information about situations in their memory. As new problems arise, similar situations are searched out to help solve these problems. Problems are understood and inferences are made by finding the closest cases in memory, comparing and contrasting the problem with those cases, making inferences based on those comparisons, and asking questions whe

  16. Synthetic Base Fluids

    Science.gov (United States)

    Brown, M.; Fotheringham, J. D.; Hoyes, T. J.; Mortier, R. M.; Orszulik, S. T.; Randles, S. J.; Stroud, P. M.

    The chemical nature and technology of the main synthetic lubricant base fluids is described, covering polyalphaolefins, alkylated aromatics, gas-to-liquid (GTL) base fluids, polybutenes, aliphatic diesters, polyolesters, polyalkylene glycols or PAGs and phosphate esters.Other synthetic lubricant base oils such as the silicones, borate esters, perfluoroethers and polyphenylene ethers are considered to have restricted applications due to either high cost or performance limitations and are not considered here.Each of the main synthetic base fluids is described for their chemical and physical properties, manufacture and production, their chemistry, key properties, applications and their implications when used in the environment.

  17. Monitoring Knowledge Base (MKB)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Monitoring Knowledge Base (MKB) is a compilation of emissions measurement and monitoring techniques associated with air pollution control devices, industrial...

  18. Soy-based polyols

    Science.gov (United States)

    Suppes, Galen; Lozada, Zueica; Lubguban, Arnold

    2013-06-25

    The invention provides processes for preparing soy-based oligomeric polyols or substituted oligomeric polyols, as well as urethane bioelasteromers comprising the oligomeric polyols or substituted oligomeric polyols.

  19. Gasification-based biomass

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2009-01-18

    The gasification-based biomass section of the Renewable Energy Technology Characterizations describes the technical and economic status of this emerging renewable energy option for electricity supply.

  20. XML-BASED REPRESENTATION

    Energy Technology Data Exchange (ETDEWEB)

    R. KELSEY

    2001-02-01

    For focused applications with limited user and use application communities, XML can be the right choice for representation. It is easy to use, maintain, and extend and enjoys wide support in commercial and research sectors. When the knowledge and information to be represented is object-based and use of that knowledge and information is a high priority, then XML-based representation should be considered. This paper discusses some of the issues involved in using XML-based representation and presents an example application that successfully uses an XML-based representation.