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Sample records for 60co irradiated tachyzoites

  1. Evaluation of immunity and protection induced in experimental models by soluble extract of Toxoplasma gondii tachyzoites irradiated by 60Co

    International Nuclear Information System (INIS)

    Toxoplasmosis affects 1/3 of the human population and only a vaccine for veterinary use. Gamma radiation alters the proteins making them more immunogenic by oxidation and better antigen presentation in the absence of adjuvants. Radiate soluble extract of RH strain tachyzoites of T. gondii (AgTg), and evaluate its use as a vaccine in BALB/c. Doses below 500Gy not affected and destroyed 2000Gy doses above extract, whereas animals immunized with irradiated extract at 1000, 1500 and 2000Gy had more of specific IgG avidity , compared to native AgTg (p<0,05) . AgTg 1500GY the immunized animals had increased proliferation of splenocytes, phenotyped as CD3+CD4+, CD3+CD8+ and B-lymphocytes immunized animals compared to the native AgTg . Animals immunized by AgTg 1500GY after challenge with strain ME- 49 cystogenic showed lower number of brain cysts and greater survival after challenge with virulent RH. Ionizing radiation in extracts of T. gondii increases the immune response and immune memory in the absence of adjuvants. (author)

  2. Toxoplasma gondii vs ionizing radiation: cell and humoral immunity in spleen and gut of isogenic mice immunized with 60Co irradiated tachyzoites

    International Nuclear Information System (INIS)

    We are developing a vaccine for toxoplasmosis, using ionizing radiation as a tool. Here we analyzed the production of systemic and intestinal immunity, with protection studies, in several strains of inbred mice, by oral or parenteral route, using 255 Gy irradiated tachyzoites of T. gondii RH strain, with challenge with cysts of ME- 49 strain. C57Bl/6j, BALB/c and C57Bl/6j IFN-γ-/- mice were immunized with 107 irradiated tachyzoites, be parenteral or oral route. Those preparations, both by parenteral or oral routes, induced the production of specific IgG, mainly of the lgG2b subclass, and IgA immunoglobulins in serum, , as determined by ELISA. IgM production was negligible. Parenteral immunized mice showed higher IgG avidity maturation, as compared to oral immunized mice. Fecal excretion of IgG, IgA and IgM was detected in stools of immunized animals, more intense in oral immunized mice. In cellular immunity studies, induced by antigen, with detection of cytokine production by quantitative real-time PCR, there are a great production of IFN-y by spleen cells, with lower levels in Peyer patches cells, where there are a greater IL-2 production. Challenge studies in immunized mice demonstrated protection to infection in all used schedules, greater in BALB/c mice. C57Bl/6j IFN-γ -/- mice, when immunized, showed no signs of disease and produced similar or greater levels of antibodies than wild type mice. They also excreted S-lgA and S-IgM in stools, but with low numbers of brain cysts in parenteral immunized mice, despite similar mortality. Our data points to a fair possibility of use of those irradiated parasites as an oral vaccine, devised to use for veterinary or wild felines vaccination, reducing the production of oocysts by those hosts and interrupting the chain transmission of human toxoplasmosis. (author)

  3. Evaluation of immunity and protection induced in experimental models by soluble extract of Toxoplasma gondii tachyzoites irradiated by {sup 60}Co; Avaliacao da imunidade e protecao induzida em modelos experimentais por extrato soluvel de taquizoitos de Toxoplasma gondii irradiado por {sup 60}Co

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    Costa, Andrea da

    2013-07-01

    Toxoplasmosis affects 1/3 of the human population and only a vaccine for veterinary use. Gamma radiation alters the proteins making them more immunogenic by oxidation and better antigen presentation in the absence of adjuvants. Radiate soluble extract of RH strain tachyzoites of T. gondii (AgTg), and evaluate its use as a vaccine in BALB/c. Doses below 500Gy not affected and destroyed 2000Gy doses above extract, whereas animals immunized with irradiated extract at 1000, 1500 and 2000Gy had more of specific IgG avidity , compared to native AgTg (p<0,05) . AgTg 1500GY the immunized animals had increased proliferation of splenocytes, phenotyped as CD3+CD4+, CD3+CD8+ and B-lymphocytes immunized animals compared to the native AgTg . Animals immunized by AgTg 1500GY after challenge with strain ME- 49 cystogenic showed lower number of brain cysts and greater survival after challenge with virulent RH. Ionizing radiation in extracts of T. gondii increases the immune response and immune memory in the absence of adjuvants. (author)

  4. Development of irradiator {sup 60}Co sources

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    Mosca, Rodrigo C.; Moura, Eduardo S.; Zeituni, Carlos A.; Mathor, Monica B., E-mail: rcmosca@usp.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    According to a recent report by the International Agency for Research on Cancer (IARC) / WHO (2008-2010), the global impact of cancer more than doubled in 30 years. In this report, it was estimated that occurred about 12 million new cancer cases and 7 million deaths. In Brazil in 2010, with estimates for the year 2011, point to the occurrence of 489,270 new cases of cancer. Among the possibilities for cancer treatment, radiotherapy is one of the most important therapeutic and resources used to combat it. However, inherent complications of treatment can occur such as tiredness, loss of appetite, radiodermatitis and in more extreme cases late radionecrosis. In order to reproduce a point of radionecrosis in the vicinity of radiodermatitis to mimic these effects in animals, producing a model for assessment of tissue repair, we propose the setting up of an irradiator source of collimated {sup 60}Co. The development of was based on 11 sources of {sup 60}Co with 1 mm thickness that were inserted by inference in stainless steel 'gate-source' screw (patent pending) and later adjusted in a cross-shaped arrangement reinforced so that the beam radiation is directed to a target point, saving for other regions around this target point. The main use of this irradiator with sources of {sup 60}Co is just one cause radionecrosis point (target point) of approximately 5 mm{sup 2} with a surrounding and adjacent area of radiodermatitis around about 8 to 10 mm{sup 2} in laboratory animals for subsequent coating with epidermal-dermal matrix populated by a cell culture of human fibroblasts, keratinocytes and mesenchymal stem cells. With that said, its use will be valuable for evaluation of curative treatments against the bone and radionecrosis or palliative treatment rather than as it is currently assumed. (author)

  5. Decontamination of fermented chicken feet by 60Co irradiation

    International Nuclear Information System (INIS)

    Fermented chicken feet was treated by 60Co irradiation, and the aerobic plate count, enumeration of coliforms, pathogens and TBARS value were measured during storage. The results showed that, aerobic plate count of all irradiated samples was lower than control, and enumeration of coliforms, and pathogens of Staphylococcus aureus, Shigella, Salmonella were not detected. TBARS value of all treatments was stable during 60 d storage. It could be concluded that 60Co irradiation of chicken feet was an effective method to prolong its shelf life. (authors)

  6. Toxoplasma gondii vs ionizing radiation: cell and humoral immunity in spleen and gut of isogenic mice immunized with {sup 60}Co irradiated tachyzoites; Toxoplasma gondii vs radiacao ionizante: imunidade humoral e celular em baco e intestino de camundongos isogenicos imunizados com taquizoitos irradiados por cobalto 60

    Energy Technology Data Exchange (ETDEWEB)

    Galisteo Junior, Andres Jimenez

    2008-07-01

    We are developing a vaccine for toxoplasmosis, using ionizing radiation as a tool. Here we analyzed the production of systemic and intestinal immunity, with protection studies, in several strains of inbred mice, by oral or parenteral route, using 255 Gy irradiated tachyzoites of T. gondii RH strain, with challenge with cysts of ME- 49 strain. C57Bl/6j, BALB/c and C57Bl/6j IFN-{gamma}{sup -/-} mice were immunized with 10{sup 7} irradiated tachyzoites, be parenteral or oral route. Those preparations, both by parenteral or oral routes, induced the production of specific IgG, mainly of the lgG2b subclass, and IgA immunoglobulins in serum, , as determined by ELISA. IgM production was negligible. Parenteral immunized mice showed higher IgG avidity maturation, as compared to oral immunized mice. Fecal excretion of IgG, IgA and IgM was detected in stools of immunized animals, more intense in oral immunized mice. In cellular immunity studies, induced by antigen, with detection of cytokine production by quantitative real-time PCR, there are a great production of IFN-y by spleen cells, with lower levels in Peyer patches cells, where there are a greater IL-2 production. Challenge studies in immunized mice demonstrated protection to infection in all used schedules, greater in BALB/c mice. C57Bl/6j IFN-{gamma} -{sup /-} mice, when immunized, showed no signs of disease and produced similar or greater levels of antibodies than wild type mice. They also excreted S-lgA and S-IgM in stools, but with low numbers of brain cysts in parenteral immunized mice, despite similar mortality. Our data points to a fair possibility of use of those irradiated parasites as an oral vaccine, devised to use for veterinary or wild felines vaccination, reducing the production of oocysts by those hosts and interrupting the chain transmission of human toxoplasmosis. (author)

  7. Radiolytic degradation scheme for 60Co-irradiated corticosteroids

    Energy Technology Data Exchange (ETDEWEB)

    Kane, M.P.; Tsuji, K.

    1983-01-01

    The cobalt 60 radiolytic degradation products have been identified in the following corticosteroids: cortisone, cortisone acetate, hydrocortisone, hydrocortisone acetate, hydrocortisone sodium succinate, isoflupredone acetate, methylprednisolone, methylprednisolone acetate, prednisolone, prednisolone acetate, and prednisone. Two major types of degradation processes have been identified: loss of the corticoid side chain on the D-ring to produce the C-17 ketone and conversion of the C-11 alcohol, if present, to the C-11 ketone. Minor degradation products derived from other changes affecting the side chain are also identified in several corticosteroids. These compounds are frequently associated in corticosteroids as process impurities or degradation compounds. No new radiolytic compounds unique to 60Co-irradiation have been found. The majority of corticosteroids have been shown to be stable to 60Co-irradiation. The rates of radiolytic degradation ranged from 0.2 to 1.4%/Mrad.

  8. Planning a 60Co Irradiation Facility for Fruit Preservation

    International Nuclear Information System (INIS)

    A conceptual design for a conveyor system is proposed for use in fruit irradiation. The seasonal nature of the fruit harvest requires that the 60Co source inventory should be sufficient to meet the demand at peak season, but this would be excessive at the beginning and towards the end of the harvest. Because of the short crop period the possibility of other irradiation services should be exploited to ensure full utilization of the facility. For successful extension of fruit shelf-life rigid practices in pre-irradiation treatment are essential and careful packaging is indispensable to the operation of the irradiator. Based on the time required for construction and equipment supply, a period of 18 months should be assumed for completion of the project. (author)

  9. Planning a 60Co irradiation facility for fruit preservation

    International Nuclear Information System (INIS)

    A conceptual design for a conveyor system is proposed for use in fruit irradiation. The seasonal nature of the fruit harvest requires that the 60Co source inventory should be sufficient to meet the demand at peak season, but this would be excessive at the beginning and towards the end of the harvest. Because of the short crop period the possibility of other irradiation services should be exploited to ensure full utilization of the facility. For successful extension of fruit shelf-life rigid practices in pre-irradiation treatment are essential and careful packaging is indispensable to the operation of the irradiator. Based on the time required for construction and equipment supply, a period of 18 months should be assumed for completion of the project. (author)

  10. Seed germination of peanuts irradiated with cobalt (60CO)

    International Nuclear Information System (INIS)

    This work was realized to evaluate the effect of gamma irradiation (60Co) at doses 0, 0.50, 1.00, 1.50, 2.00, 2.50, 3.00 and 4.00 kGy, on germination of seeds of peanut, cultivar BR1. Irradiation Department of Nuclear, UFPE, where he received after the irradiation, they were stored in packing of PET and polyethylene braided with a time of 90 days. Through the results, obtained monthly, concluded that the dose of 0.5 kGy was effective in the germination of seeds of peanut, not affecting its power of germination and overcoming the witness within 30 to 60 days. (author)

  11. Response of adrenal gland to whole body 60Co irradiation

    International Nuclear Information System (INIS)

    Whole body of the adult albino rates was exposed to 60Co radiation in a single dose of 600 R. Following irradiation the adrenal serotonin level was found higher till the end of 8th week except a fall on 14th day, whereas the blood 5HT level remained lower than the normal except a slight rise at the end of 1st week and dropped down at 14 days followed by a further rise. The blood catecholamine level was found increased at the end of 14th day followed by a fall at 4th and 8th weeks, but the levels were moving round the normal value. The histological studies of adrenal gland showed degranulation and hypertrophy of adrenal cortex and medullary cells at various intervals of post-irradiation. On the whole it is observed that maximum changes in the level of biogenic amines take place within 14 days after irradiation, and maximum rate of mortality also coincide with this period. Thus bringing out the fact that adrenal bioamines play an important role in the vital activities of the animals. (author)

  12. Effect of 60Co-gamma whole-body irradiation on serum amylase level

    International Nuclear Information System (INIS)

    Changes of serum amylase activity in rats, after several doses of acut 60Co-gamma irradiation as a function of time were investigated. These changes proved to be of no diagnostic value in early radiation damage. (author)

  13. Manufacture of 60Co source irradiation facility and measurement of radiation filed

    International Nuclear Information System (INIS)

    60Co source radiation facility is an important equipment to produce reference radiation filed, according to national standard, the scattered dose rate must be less than 5% of the total dose rate in the reference radiation filed. Scattered radiation contribution and uniformity of reference radiation field are important parameters to describe the radiation performance of 60CO source irradiation device, Monte Carlo method was used to study the scattered radiation and uniformity of the reference radiation filed to provide theoretical basis for the design of 60CO source irradiating device in order to avoid economic losses caused by design mistakes. When 60CO source irradiating device was manufactured, PTW ionization chamber was used to measure the irradiation field. The results showed that the scattered radiation and uniformity of the radiation filed were in good agreement with the simulation results. The radiation performance met the design requirements. (authors)

  14. Detection of Argentine onions treated with 60 Co irradiation

    International Nuclear Information System (INIS)

    Brazil has been the most important MERCOSUL's purchaser of fresh onions from Argentina. The increased claim for this fresh product has forced a consensus between the members nations, as regards to phytosanitary restrictions. The radio inhibition is described on National Food Codes in Brazil and Argentina. Methods of food irradiation detection must be performed, since they increase the consumer confidence. Quick and simple screening tests indicate whether a food product has been irradiated or not. This present study verified the DNA fragments of argentine fresh onions, produced during radiation process and 6 months of storage period. The DNA fragments are analyzed for detection of irradiated foods. The irradiated onions presented extensive DNA migrations, as comets, when submitted to agarose gel electrophoresis. They also showed more shelf life compared to the unirradiated onions. The unirradiated samples exhibited only limited DNA migration. This initial screen method showed to be effective for detection of irradiated onions. (author)

  15. Immunohistochemical study on effects of [sup 60]Co [gamma] irradiation on salivary gland ducts of rat

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    Saitoh, Kazuhiko (Meikai Univ., Sakato, Saitama (Japan). School of Dentistry)

    1992-09-01

    Cytokeratin distribution in salivary glands was detected by use of polyclonal antikeratin antiserum (TK) and monoclonal antibodies (KL 1, RGE 53, and RPN 1164). The salivary glands of male rats received either 17.82 Gy or 27.97 Gy [sup 60]Co [gamma] irradiation in a single exposure and were then compared immunohistochemically with those of normal rats. Polyclonal anti-keratin antiserum (TK), which reacts with 41-65 KD keratins, stained almost all ducts in normal glands. RPN 1164 (no. 8 keratin) staining was negative in intercalated ducts of normal parotid and submandibular glands, but strongly positive in both striated and excretory ducts of these glands. Monoclonal antibody KL 1 (55-57 KD keratins) and RGE 53 (no. 18 keratin) did not bind to any ductal or acinar epithelia. Only in the sublingual gland were acini positive for TK staining, possibly indicating myoepithelial cells. No effects of [sup 60]Co [gamma] irradiation were apparent regarding keratin distribution in the intercalated in apical cytoplasm by [sup 60]Co [gamma] irradiation. Also, in the excretory duct, the basal side of the cells exhibited weakened staining following [sup 60]Co [gamma] irradiation were the most significant in the parotid and the least in the sublingual gland. Also this reaction depended upon the doses of [sup 60]Co used. The present findings suggested that negative or weakened staining at the basal and perinuclear portions of striated duct cells specifically reflects the primary or secondary cell damage produced by [sup 60]Co [gamma] irradiation. Since the distribution of cytoskeletal proteins in the cytoplasm reflects certain pathological conditions, immunohistochemical detection of these proteins seem to have a diagnostic value with respect to cellular injury. (J.P.N.) 77 refs.

  16. SV40 DNA amplification and reintegration in surviving hamster cells after 60Co γ-irradiation

    International Nuclear Information System (INIS)

    SV40-transformed Chinese hamster embryo cells were exposed to 60Co γ-irradiation and the fate of the integrated SV40 sequences was pursued over a period of 20 days following radiation exposure. As shown by colony hybridization, integrated SV40 sequences were amplified in surviving and non-surviving cells. At later times, however, clonal sublines of surviving cells grown for 20-30 cell generations after irradiation had lost most of their amplified SV40 copies but showed altered restriction fragment patterns indicating reintegration of SV40 sequences at new sites of the hamster genome. This suggest that 60Co γ-irradiation can generate mutations by inducing over-replication of chromosome segments that are then substrates of enzymatic rearrangements. (author)

  17. Effect of 60Co-γ ray irradiation on green coffee beans, 1

    International Nuclear Information System (INIS)

    Green coffee beans were irradiated with 60Co-γ rays at doses of 0, 0.05, 0.5, 5.0 and 10.0 Mrad and the changes of general components in green and roast coffee beans were investigated together with those of the organoleptic properties of roast beans during storage according to the cup testing. In case of Brazil santos beans, irradiation of some 0.05 Mrad 60Co-γ ray gave rather favourable mild flavour and no harmful influence on the quality of coffee, and moreover, would tend to extend the shelf life of roast beans. But influence of irradiation on the quality of coffee differed somewhat between two cultivars, Brazil santos and Colombia. (auth.)

  18. Experiments for inactivation of mycoplasms and bacteria in calf sera, using 60Co irradiation

    International Nuclear Information System (INIS)

    The use of 60Co gamma radiation to inactivate mycoplasms in calf serum, newborn calf serum, and fetal calf serum is reported. A dose of 3 kGy, independent of dose rate, was found to be sufficient for inactivation in the above sera of several mycoplasms, including Acholeplasma laidlawii, Mycoplasma orale, M. arginini, M. hyorhinis, and M. bovis. The critical dose proved to be at 2 kGy. No difference was found to exist between the above species in susceptibility to irradiation in diluted sera (50% and 10% in Eagle MEM). Sensibility of wild mycoplasm strains was found to be identical with that of laboratory strains. Hence, 60Co gamma irradiation of sera appears to be a safe method by which to make sera free of mycoplasms. Bacillus subtilis in calf serum was inactivated by doses above 18 kGy, with the critical dose being 15 kGy. (author)

  19. Effect of Vaccination with Irradiated Tachyzoites on Histopathological Changes and DNA Damage in Hepatocytes of Experimental Toxoplasmosis

    International Nuclear Information System (INIS)

    Current strategies for the control of toxoplasmosis are based on chemotherapy, however successful vaccine has also been demonstrated. The present study aims to assess the effect of the vaccination with radiation-attenuated tachyzoites in challenged mice regarding histopathological alteration and DNA damage of hepatocytes. Sixty mice were equally divided as follow: Group I left as a normal control group II was infected with 2x103 RH virulent tachyzoite s (infected control). Groups III and IV were subdivided into two subgroups a and b where subgroups IIIa and IVa were vaccinate d with 2.47 mw-min/cm2 UV and 0.3 KGy gamma radiation – attenuate d tachyzoites respectively without challenge (as vaccine control). Subgroups III b and IV b were vaccinate d with UV and gamma radiation - attenuated tachyzoites and challenged after three weeks with 2x103 RH virulent tachyzoites. Livers were examined for histopathological changes and DNA comet assay. It was observed that acute infection with Toxoplasma tachyzoites produced toxic effects which lead to severe damage in liver tissues and DNA of hepatocytes. Meanwhile, the protective effect of UV or gamma radiation-attenuated tachyzoites vaccine resulted in the maintenance of normal histopathological characteristics and DNA of hepatocyte s and UV irradiation is better in its protective capacity

  20. Effects of 60Co-γ ray irradiation on antioxidant enzyme activities in tobacco

    International Nuclear Information System (INIS)

    Effects of 300Gy 60Co-γ ray irradiation on the activities of 3 antioxidant enzymes in tobacco were studied in this paper. The results showed that the activities of superoxide dismutase (SOD), peroxidas (POD) and catalse (CAT) in tobacco were gradually increased. All the activities of SOD POD and CAT reached the maximums at 12 h and then gradually decreased. Similar results were also observed in the expression of sod, pod and cat genes. These results indicated that the activities of antioxidant enzymes could play an important role in tobacco tolerance against irradiation

  1. Effect of 60Co-irradiation on penicillin G procaine in veterinary mastitis products

    International Nuclear Information System (INIS)

    The effect of 60Co-irradation on penicillin G procaine in a peanut oil-based veterinary mastitis product was examined by reversed-phase high-performance liquid chromatography (HPLC). The HPLC method is capable of separating and quantifiying procaine, penicillin G, and various degradation compounds. Values obtained by the HPLC method on the product irradiated and stored at various temperatures correlated well with those of the microbiological assay. No significant decrease in the procaine was detected even after 4.0-Mrad irradiation. The HPLC method is applicable for analysis of other beta-lactam antibiotics

  2. Protective effect of gingerol on leucocyte and bone marrow DNA of 60Co γ-rays irradiated mice

    International Nuclear Information System (INIS)

    In this article, the effect of gingerol on peripheral leucocyte and bone marrow DNA of 60Co γ-rays irradiated mice was developed., Twenty-four healthy healthy female Kunming mice were randomly divided into 4 groups: control, gingerol, irradiation and gingerol + irradiation group. Gingerol group and gingerol + irradiation group were given gingerol intragastrically once a day for five days. Irradiation group and gingerol + irradiation group were suffered from 5 Gy 60Co γ-rays irradiation at the rate of 1.2 Gy/min on the 6th day. Blood samples, spleens, livers and thigh bones were collected to be measured after 48 h. The results showed that, compared with irradiation group, gingerol + irradiation group had significantly higher spleen index (p60Co γ-rays irradiated mice. (authors)

  3. Effects of testicular irradiation with 60Co in guinea pigs CAVIA PORCELLUS

    International Nuclear Information System (INIS)

    Improvements were made in restaint systems and in the technique of electroejaculation in guinea pigs, emphasizing safety, effectiveness and repetition. Arrangements for simultaneous testicular irradiation with 60Co in 5 animals and a phantom to test dose levels in the target region and scattering were also made. Effects of single and fractionated exposures on sperm were studied. A collimated point-shaped 60Co-machine calibrated to 7 R/min was used as radiation source. Groups of five 12 month aged male guinea pigs were exposed to either single testicular irradiation of 50, 100, 200 and 400 R or fractionated weekly testicular irradiation of 2, 5, 10 and 20 R, during 10 weeks. A last group was exposed to 4 fractionated testicular irradiations of 20 R at time intervals of 21 days and a group of 8 animals was used as control. Semen samples evaluations were made during 5 weeks before irradiation and in the subsequent 11 weeks. Abnormalities in sperm morphology were studied by William's method and phase contrast microscopy. Mean percentages of semen normal espermatzoons and spermatic abnormalities are shown in figures and photomicrographs. Marked differences on concentration, sperm motility and sperm abnormalities were found, but not on volume, in favour of 100 and 200 R single doses levels, as compared to fractionated 10 and 20 R. (Author)

  4. Effects of 60Co γ-rays irradiation on seed growth of ground-cover chrysanthemum

    International Nuclear Information System (INIS)

    The seeds of ground-cover chrysanthemum were used to study the effects of different doses of 60Co γ-rays irradiation(10-50 Gy) on seed germination and physiological characteristics. The results showed that the rate of seed germination and seedling survival decreased significantly with the irradiation doses. With the increase of irradiation dose to above 20 Gy, the content of malondialdehyde (MDA) and activity of peroxidase (POD) in seedlings significantly increased. The similar trends were found in the activities of superoxide dismutase (SOD) and glutathione reductase (GR). Catalase (CAT) activity increased at doses lower than 20 Gy, and then decreased at the higher doses, whereas ascorbate peroxidase (APX) activity did not alter except for 40 Gy. It is concluded that the suitable irradiation dose of mutation breeding is 20 Gy for the seeds of ground-cover chrysanthemum. Although 60Co γ-rays irradiation resulted in damage of membrane lipid peroxidation in the survival seedlings, the increased activity of CAT and POD could protect them against the damage. (authors)

  5. Distribution of 60Co and 54Mn in graphite material of irradiated HTGR fuel assemblies

    International Nuclear Information System (INIS)

    Distribution of 60Co and 54Mn was measured in the graphite sleeves and blocks of the third and fourth HTGR fuel assemblies irradiated in the Oarai Gas Loop-1 (OGL-1), which is a high temperature inpile gas loop installed in the Japan Materials Testing Reactor (JMTR) of Japan Atomic Energy Research Institute (JAERI). Axial and circumferential profiles were obtained by gamma spectrometry, and radial profiles by lathe sectioning with gamma spectrometry. Distribution of 60Co is in good agreement with that of thermal neutron flux, and the Co content in the graphite is estimated to be -- 1 x 10-9 in weight fraction. Concentration of 54Mn decreases toward the axial center in its axial profile, and radially is almost uniform inside and appreciably higher at free surfaces. An estimated Fe content of --10-8 in wight fraction is smaller by two orders of magnitude than that from chemical analysis. Higher concentraion of 60Co and 54Mn at the free surfaces suggests the importance of transportation process of these nuclides in the coolant loop. (author)

  6. Cell survival of human tumor cells compared with normal fibroblasts following 60Co gamma irradiation

    International Nuclear Information System (INIS)

    Three tumor cell lines, two of which were shown to be HeLa cells, were irradiated with 60Co gamma irradiation, together with two cell cultures of normal human diploid fibroblasts. Cell survival was studied in three different experiments over a dose range of 2 to 14 gray. All the tumor cell lines showed a very wide shoulder in the dose response curves in contrast to the extremely narrow shoulder of the normal fibroblasts. In addition, the D/sub o/ values for the tumor cell lines were somewhat greater. These two characteristics of the dose response curves resulted in up to 2 orders of magnitude less sensitivity for cell inactivation of HeLa cells when compared with normal cells at high doses (10 gray). Because of these large differences, the extrapolation of results from the irradiation of HeLa cells concerning the mechanisms of normal cell killing should be interpreted with great caution

  7. Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom

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    Lucas de Oliveira, P.C.; Madruga, R.A.; Barbosa, N.P.U. [Uberaba School of Veterinary Medicine (UNIUBE), MG (Brazil)]. E-mail: pedrolucaso@uol.com.br; Sakate, M. [UNESP, Botucatu, SP (Brazil). School of Veterinary Medicine and Animal Husbandry

    2007-07-01

    Venoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals to increase antigenic induction and reduce the envenomation pathological effects. Gamma radiation from {sup 60}Co has been used as an attenuating agent of the venoms toxic properties. The main objective was to study, comparatively, clinical and laboratory aspects of goats inoculated with bothropic (Bothrops jararaca) venom, natural and irradiated from a {sup 60}Co source. Twelve goats were divided into two groups of six animals: GINV, inoculated with 0.5 mg/kg of natural venom; and GIIV, inoculated with 0.5 mg/kg of irradiated venom. Blood samples were collected immediately before and one, two, seven, and thirty days after venom injection. Local lesions were daily evaluated. The following exams were carried out: blood tests; biochemical tests of urea, creatinine, creatine kinase, aspartate amino-transferase and alanine amino-transferase; clotting time; platelets count; and total serum immunoglobulin measurement. In the conditions of the present experiment, irradiated venom was less aggressive and more immunogenic than natural venom. (author)

  8. Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom

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    P. C. Lucas de Oliveira

    2007-01-01

    Full Text Available Venoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals to increase antigenic induction and reduce the envenomation pathological effects. Gamma radiation from 60Co has been used as an attenuating agent of the venoms toxic properties. The main objective was to study, comparatively, clinical and laboratory aspects of goats inoculated with bothropic (Bothrops jararaca venom, natural and irradiated from a 60Co source. Twelve goats were divided into two groups of six animals: GINV, inoculated with 0.5mg/kg of natural venom; and GIIV, inoculated with 0.5mg/kg of irradiated venom. Blood samples were collected immediately before and one, two, seven, and thirty days after venom injection. Local lesions were daily evaluated. The following exams were carried out: blood tests; biochemical tests of urea, creatinine, creatine kinase (CK, aspartate amino-transferase (AST and alanine amino-transferase (ALT; clotting time; platelets count; and total serum immunoglobulin measurement. In the conditions of the present experiment, irradiated venom was less aggressive and more immunogenic than natural venom.

  9. Humoral immune response against native or 60Co irradiated venom and mucus from stingray Paratrygon aiereba

    International Nuclear Information System (INIS)

    Poisonings and traumas caused by poisonous freshwater fish such as rays are considered a major public health problem and draw attention because of accidents involving these animals cause serious local symptoms and are disabling, keeping the victim away from work. The therapy of these cases is based only on the symptoms of patients, which implies in its low efficiency, causing suffering for the victims. This study aims to evaluate and compare the humoral immune response in animals inoculated with native or 60Co irradiated Paratrygon aiereba venom and mucus. Ionizing radiation has proven to be an excellent tool to decrease the toxicity of venoms and isolated toxins. The mucus and venom samples of P. aiereba were irradiated using gamma rays from a 60Co source. Animals models were immunized with the native or irradiated mucus or venom. The assays were conducted to assess the production of antibodies by the immunized animals using enzyme immunoassay and western blotting. Preliminary results show the production of antibodies by the immunized animals. The resulting sera were also checked for antigenic cross- reactivity between venom and mucus, demonstrating the potential of mucus as an antigen for serum production for the specific treatment for accidents by stingrays. However, it is essential to carry out further tests in order to verify the neutralization of the toxin by antibodies formed by animals. (author)

  10. Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom

    International Nuclear Information System (INIS)

    Venoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals to increase antigenic induction and reduce the envenomation pathological effects. Gamma radiation from 60Co has been used as an attenuating agent of the venoms toxic properties. The main objective was to study, comparatively, clinical and laboratory aspects of goats inoculated with bothropic (Bothrops jararaca) venom, natural and irradiated from a 60Co source. Twelve goats were divided into two groups of six animals: GINV, inoculated with 0.5 mg/kg of natural venom; and GIIV, inoculated with 0.5 mg/kg of irradiated venom. Blood samples were collected immediately before and one, two, seven, and thirty days after venom injection. Local lesions were daily evaluated. The following exams were carried out: blood tests; biochemical tests of urea, creatinine, creatine kinase, aspartate amino-transferase and alanine amino-transferase; clotting time; platelets count; and total serum immunoglobulin measurement. In the conditions of the present experiment, irradiated venom was less aggressive and more immunogenic than natural venom. (author)

  11. The influence of 60Co gamma irradiation for downy mildew infection on corn

    International Nuclear Information System (INIS)

    Corn seeds of Genjah kertas variety were irradiated with 60Co gamma in the range of 0 rad - 40.000 rads, at interval of 2000 rads, as preliminary investigation to obtain possibilities of corn mutation. Several agronomical effects of radiation on the seedling-plant such as seed germination, height of the seedling, ages of flowering, ages of harvesting, length and circle of the cob, 100U dry seeds weight, number and diameter of stomata, and percentage of downy mildew infection have been observed. The exact conclusion can be obtained only after testing the results of M2 and M3. (author)

  12. Study on Several Characteristics of Agaricus blazei Murril Strain J3 Irradiated by 60Co

    Institute of Scientific and Technical Information of China (English)

    WENG Bo-qi; JIANG Zhi-he; HUANG Ting-jun; CHEN Jian; ZHENG Wei-wen

    2003-01-01

    A new mutant strain J3 from Agaricus blazei Murril was obtained by 60Co irradiation. The yields of successive generations were increased more than 70% to compare with their original strain. The component analysis on amino acids and fatty acid illuminated that the nutrient value of strain J3 fruiting body was better than the original strain. The apparent nodule structure was found in the hyphea of J3 strain through the observation by scanning electron microscope. RAPD analysis showed the great difference of PCR fingerprints between J3 and its original strain. It is a promising mutant strain for further commercial development in the future.

  13. Study for optimization of a 60 Co industrial irradiator of 250 k Ci

    International Nuclear Information System (INIS)

    This work presents results of a benchmark between calculated and experimental absorbed dose values for a typical product, in a 60 Co industrial irradiator, located at ININ, Mexico. The goal of this work is to reach a ± 10 % approximation between both values. ININ 60 Co industrial irradiator is a two level, two layer system with overlapping product configuration, 56 irradiation positions with an activity of around 250 k Ci. Calculated values were obtained by QAD-CGGP code. This code uses a point kernel technique, build-up factors fitting was done by geometrical progression and combinatorial geometry was used for 3 D system description. Main code modifications were related with source simulation by punctual sources and energy spectrum (16 energy groups) and anisotropic emission were also considered. Experimental data were obtained from routine dosimetry which was done with red acrylic pellets; they were irradiated together the product in predetermined positions, for 36 maximum and minimum absorbed dose values. Typical product was polypropylene Petri dish packages, apparent density 0.13 g/cm3. It was chosen because regular geometry (2 packages per full irradiator container) and enough amount for considering homogeneous loading inside irradiation chamber. Required minimum dose was 15 kGy. Results showed a 8% variation between calculated and experimental values for maximum absorbed dose (18.2 kGy vs 16.8 kGy) and 3 % variation for minimum absorbed dose (13.8 kGy vs 14.3 kGy); these results fixed with the original proposal. (Author)

  14. 60Co irradiation for sterilization of veterinary mastitis products containing antibiotics and steroids

    Science.gov (United States)

    Tsuji, K.; Kane, M. P.; Rahn, P. D.; Steindler, K. A.

    Effects of 60Co irradiation for sterilization of veterinary mastitis products were evaluated. The mastitis products which were examined contained various combinations of antibiotics and steroids suspended in peanut oil vehicle. Bioburden data indicated that the unirradiated products were only occasionally contaminated with microorganisms. The D-values of the nonsterile product and environmental isolates were 0.028, 0.15, 0.017, and 0.018 Mrads for Aspergillus fumigatus, Penicillium oxalicum, Pseudomonas aeruginosa, and Pseudomonas maltophilia, respectively. The D-value of the biological indicator organism, Bacillus pumilus spores, in the vehicle was 0.27 Mrads. Thus, an irradiation dose of 1.6 Mrads would be sufficient to achieve six log cycles of destruction of the biological indicator organism. The minimum absorbed irradiation dose of 2.5 Mrads preferred by many countries for sterilization would achieve 9.3 log cycle destruction of the indicator organism and guarantee a probability of 1 × 10 -15 assurance for the most radio-resistant product isolate, Penicillium oxalicum. In order to examine short and long term chemical stabilities of active components, stability indicating high-performance liquid chromatographic (HPLC) methods for the determination of the following antibiotics and steroids were developed. They were: dihydrostreptomycin, neomycin, novobiocin, penicillin G, hydrocortisone acetate, hydrocortisone sodium succinate, and prednisolone. The rates of degradation and radiolytic degradation schemes for the majority of these compounds were elucidated. Formation of new compounds was not observed in these antibiotics and steroids upon 60Co irradiation. The compounds that increased by irradiation were inherently present in commercially available non-irradiated lots and/or can easily be formed by either acidic, basic, or thermal treatment.

  15. Seed germination of peanuts irradiated with cobalt ({sup 60}CO); Germinacao de sementes de amendoim irradiadas com cobalto ({sup 60}Co)

    Energy Technology Data Exchange (ETDEWEB)

    Alves, Niedja Marrize C.; Almeida, Francisco de Assis C.; Gomes, Josivanda P.; Pessoa, Elvira B., E-mail: niedjamarizze@yahoo.com.b, E-mail: josivanda@deag.ufcg.edu.b, E-mail: elvirabe@gmail.co [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Dept. de Engenharia Agricola; Leal, Artur S. Cavalcanti, E-mail: arturcleal@yahoo.com.b [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Dept. de Engenharia de Materiais

    2010-07-01

    This work was realized to evaluate the effect of gamma irradiation ({sup 60}Co) at doses 0, 0.50, 1.00, 1.50, 2.00, 2.50, 3.00 and 4.00 kGy, on germination of seeds of peanut, cultivar BR1. Irradiation Department of Nuclear, UFPE, where he received after the irradiation, they were stored in packing of PET and polyethylene braided with a time of 90 days. Through the results, obtained monthly, concluded that the dose of 0.5 kGy was effective in the germination of seeds of peanut, not affecting its power of germination and overcoming the witness within 30 to 60 days. (author)

  16. Studies on mutagenic activity of 60Co γ-ray irradiated rape pollen

    International Nuclear Information System (INIS)

    In the present study on disinfection, the rape pollen was irradiated with 2.5 kGy 60Co γ-ray. Micronuclei, sister chromatid exchanges (SCE) of bone marrow cells and chromosomal aberrations of meiotic cells in mice were used as an indicater of chromosomal damage to study the mutagenicity of irradiated rape pollen. The results are as follows: (1) The frequency of micronuclei in polychromatic erythrocytes is 2.00 per mille; nucleated cells is 0.8 per mille in control group. In the numbers of polychromatic erythrocytes and nucleated cells with micronuclei, there is no obviously difference in irradiated and unirradiated groups. (2) SCE incidence of control group is 2.01 ± 0.12/cell. No significant difference in the frequency of SCE exists between non-irradiated rape pollen and the control groups. But the frequency of SCE in irradiated rape pollen group (3000 mg/kg/day x 7) is 2.36 ± 0.12/cell; high dose group (6000 mg/kg/day x 7) is 2.96 ± 0.14/cell. In comparison with control group, there is a significant difference. (3) The chromatid breaks, fragments, and univalents in primary spermatocytes have been obseved. The frequencies of chromosomal aberration showed no obviously difference among irradiated and non-irradiated rape pollen groups

  17. 60CoIrradiation Affects the Enzymatic Antioxidant System of the Citrus Red Mite Panonychus citri (Acari: Tetranychidae)

    OpenAIRE

    Ke Zhang; Zhibin Li; Shaowen Zhu; Qunfang Weng

    2014-01-01

    Radio-(60Co), which emits γ rays, has been used worldwide in pest control. The aim of the present study was to analyze the effect of effective-low-power 60Coirradiation on the enzymatic antioxidant system of the citrus red mite Panonychus citri. One day old female adults were exposed to 0.4 kGy 60Coirradiation and on the, 6th h, 1st day, 2nd day, and 5th day post treatment, the mites were euthanized for biochemical analysis. The activity of superoxide dismutase (SOD), catalase (CAT), p...

  18. Primary Observations on the Influence of 60Co Local Irradiation on Free Skin Flaps

    Institute of Scientific and Technical Information of China (English)

    杨建荣; 张园

    2002-01-01

    Objective To study the effect of postoperative radiation on free skin flaps.Methods Twenty-nine patients with free skin flaps applied to the plerosis of the postoperative defectwere followed up. Twenty-eight out of 29 patients received forearm free flaps while 1 had anterolater-al femoris skin flaps in a size ranging from 14 cm× 6 cm to 8 cm× 4 cm. These flaps were exposedto 60Co radiation ranging 40-69 Gy. Results Observation during irradiation: no visible changes ofskin flaps and oral mucous membrane were detected when irradiation dose was within 40 Gy; somechanges may be detected over 40 Gy. Observation 1 - 4 months after irradiation: pigmentations werefound in areas of erosion epidermis; skin flaps got dark but without evidence of necrosis. Observation2 years after irradiation: no necrosis happened in 29 skin flaps. Hairs were found on 15 of 29flaps. Sensation occurred within the margin of 1 cm. Conclusion Free skin flap can endure irradi-ation at the dose of 60 Gy.

  19. Biochemical and immunological alterations of 60 Co irradiated Bothrops jararacussu venom

    International Nuclear Information System (INIS)

    Proteins irradiation leads to structural alterations resulting in activity and function loss. This process has been useful to detoxify animal venoms and toxins, resulting in low toxicity products which increased immunogenicity. The Bothrops jararacussu venom behaves as a weak immunogen and its lethal activity in not neutralized by either autologous, heterologous or bothropic polyvalent antisera. This venom is markedly myotoxic and and the commercial bothropic antiserum does not neutralize this activity, because of this low immunogenicity of the myotoxins. This present work was done in order to evaluate the possibility of irradiating Bothrops jararacussu, intending to increase the immunogenicity of the myotoxic components, leading to productions of myotoxins neutralizing antibodies. Bothrops jararacussu venom samples were irradiated with 500, 1000 and 2000 Gy of 60 Co gamma rays. A 2.3 folds decrease of toxicity was observed for the 1000 Gy irradiated samples while the 2000 Gy irradiated sample was at least 3.7 folds attenuated. On the other hand, the 500 Gy did not promote any detoxification. Electrophoresis and HPLC data indicate that the irradiation lead to the formation of high molecular weight products (aggregates). The proteolytic and phospholipasic activities decreased in a dose dependent manner, the phospholipases being more resistant than the proteases. Both the animals (rabbit) immunized with either native or 2000 Gy irradiated venom produced native venom binding antibodies, a slightly higher titer being obtained in the serum of the rabbit immunized with the irradiated samples. Western blot data indicate that the anti-irradiated venom Ig Gs recognised a greater amount of either autologous or heterologous venom bands, both sera behaving as genus specific. The anti-native serum did not neutralize the myotoxic activity of native venom, while the anti-irradiated one was able to neutralize this activity. (author)

  20. Gamma 60Co-irradiation of organic matter in the Phosphoria Retort Shale

    Science.gov (United States)

    Lewan, M. D.; Ulmishek, G. F.; Harrison, W.; Schreiner, F.

    1991-04-01

    Irradiation experiments were conducted on a thermally immature rock sample of the Phosphoria Retort Shale and its isolated kerogen. A 60Co-source for gamma radiation was employed at dosages ranging from 81 to 885 Mrads, which are attainable by Paleozoic and Precambrian black shales with syngenetic uranium enrichments. Kerogen elemental, isotopic, and pyrolysate compositions are not affected at these dosages, but the bitumens extracted from the irradiated rock are affected. The major effects are reductions in the amounts of bitumen, acyclic isoprenoids, and high-molecular weight acyclic carboxylic acids. Natural differences in the amounts of bitumen and acyclic isoprenoid due to regional and stratigraphie variations in organic source input and depositional conditions make the radiation-induced reductions in these parameters difficult to use as indicators of natural radiation damage in black shales. However, the preferential reduction in the high-molecular weight acyclic carboxylic acids, which are ubiquitous in the living precursory organic matter, is diagnostic of experimental γ-irradiation but may not be diagnostic of natural irradiation. The overall process associated with radiation damage is polymerization by cross-linking through a free radical mechanism. As a result, irradiation of organic matter in black shales is more likely to retard rather than enhance petroleum generation.

  1. Evaluation by EPR of potential antioxidant components of 60Co-irradiated varieties of soybean

    International Nuclear Information System (INIS)

    Brazil is today the second main producer of soybean in the world with a planted ground of about 21 million hectares and an annual production of 60 million tons in 2008, being slight more than a fourth of the annual production. The presence of flavonoids, particularly isoflavones in soybean products has been related as important for human health. It has been suggested that flavonoids play a role in the protection of plants by screening vital cellular components from damaging UV radiation. Electron paramagnetic resonance (EPR) spectroscopy can measure free radicals produced by dissociation molecules resulting from irradiation. It has been successfully employed for the detection of some irradiated food products. Twenty one Brazilian soybean cultivars from two crops were gamma-irradiated with a 60Co source and evaluated by EPR. Correlation coefficients were made among the central EPR signal (g = 2.0039) and the total and partial isoflavones contents. There was no correlation with total contents, though glicitein and acetyl-daidzin showed a negative correlation. Even 7 months after irradiation the intensity of central EPR signal were high enough to distinguish the irradiated samples. EPR measurements of separated parts of the grain were more efficient for that purpose, particularly from hilum and coat. The radiation did not change substantially the total isoflavone contents, although there were some evidences suggesting some conversion of glycosides to aglycones. (author)

  2. Irradiation effect on enzymatic activity of papain with {sup 60}Co-{gamma} rays

    Energy Technology Data Exchange (ETDEWEB)

    Furuta, Masakazu; Ohashi, Isao; Oka, Masahito; Hayashi, Toshio [Osaka Prefecture Univ., Sakai (Japan). Research Inst. for Advanced Science and Technology

    1998-12-31

    An investigation was made on the durability of enzyme activity against {sup 60}Co-{gamma} irradiation at a dose up to 55 kGy/h using dry powder and aqueous solution of papain preparations on the market. Hybrid materials including bioactive molecules combined with biocompatible synthetic polymers are expected to have biocompatible properties and also biomimetic functions as a component of artificial organs for human body. The activity of papain in an aqueous solution was rapidly decreased at the early stage of irradiation through oxidation of SH group at its active site with active oxygen produced by the irradiation and then, partially recovered since SH group was reproduced in an anoxic state after O{sub 2} consumption in the solution irradiated at a high dose. A usual radiation method for sterilization was found applicable to decontamination of dry and frozen preparations of papain. When suitable conditions for radiation were chosen and N{sub 2} gas was purged to suppress the formation of free radicals, it was possible to keep the enzyme activity at more than 50% of the initial activity after radiation at 30 kGy. (M.N.)

  3. Proteomics study of progeny of normal human liver cells irradiated by 60Co γ-rays

    International Nuclear Information System (INIS)

    Objective: To characterize the differential protein expression in the progeny of human liver cells surviving from ionizing radiation by the proteomic analysis. Methods: Two-dimensional electrophoresis gel coupled with mass spectrometry was used to explore the specific protein expression in the progeny of 7702 human liver cells surviving from ionizing radiation. Alterations in expression level of protein spots between the control and the progeny groups were statistically analyzed by ImageMaster 2D Platinum software and mass spectrometry was used to identify the protein spots with significantly altered expression-level. Results: The progeny of irradiated ceils were derived from human liver cell line exposed to 0, 2, 4, 6 Gy of 60Co γ-irradiation. A total of 42 differentially expressed proteins between the control and the progeny of the irradiated cells groups were screened, of which 17 were identified by matrix assistant laser desorption ion-top off light-mass spectrometry (MALDI-TOF MS) analysis, including 4 up-regulated and 13 down-regnlated proteins. Conclusions: The differentially expressed proteins profile could be significantly altered in the progeny of irradiated cells. The proteomics approach has the potential to detect the protein changes relevant to radiatian-induced genomic instability (RIGI). Further study of differentially expressed proteins would likely reveal the molecular mechanisms of gene expression in RIGI. (authors)

  4. Decomposition of ρ-nonylphenols in water by 60Co γ-ray irradiation

    International Nuclear Information System (INIS)

    ρ-Nonylphenols (NPs), one of endocrine disrupting chemicals, are used as plastic flexibilizers or nonionic surfactants, and widely released into the water environment. Hydroxyl radicals produced from water molecules by γ-ray irradiation have high oxidation reactivity. Recently, treatments with the hydroxyl radicals have drawn much attention to conserve the water environment. In this study, decompositions of NPs in water were investigated using hydroxyl radicals by 60Co γ-rays irradiation. The concentrations of the NPs at initial concentration from 45 to 1000 nM were decomposed by γ-ray irradiation. Qualitative and quantitative analyses of NPs were carried out by high performance liquid chromatography. The decomposition curves of NPs at each initial concentration were analyzed as single exponential functions. Alkylphenol activity of aqueous NPs solution, which was estimated by enzyme-linked immunosorbent assay, implies the irradiation products have alkylphenol activity. Two products having molecular weight of 236 were investigated by liquid chromatography-mass spectrometry, and were considered to be ρ-nonylcatechol and 1-(ρ-hydroxyphenyl)-1-nonanol on the basis of the oxidation mechanisms of ρ-cresol and 4-ethylphenol. (author)

  5. Study of irradiated bothropstoxin-1 with {sup 60}Co gamma rays: immune system behavior

    Energy Technology Data Exchange (ETDEWEB)

    Caproni, P.; Baptista, J.A.; Almeida, T.L. de; Nascimento, N. [Nuclear and Energy Research Institute (IPEN/CNEN-SP), SP (Brazil). Biotechnology Center; Passos, L.A.C. [State University of Campinas (UNICAMP), SP (Brazil). Lab. of Genetics and Embryo Cryopreservation

    2009-07-01

    Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules. Very promising results were obtained when crude animal venoms, as well as isolated toxins, were treated with 60Co gamma rays, yielding toxoids with good immunogenicity. The achievement of modified antigens with lower toxicity and preserved or improved immunogenicity can be very useful. Ionizing radiation has already been proven to be a powerful tool to attenuate snake venom toxicity without affecting, and even increasing, their immunogenic properties. However, little is known about the modifications that irradiated molecules undergo and even less about the immunological response that such antigens elicit. In the present work, we investigated the immunological behavior of bothropstoxin-1, a K49 phospholipase, before and after irradiation. Structural modifications of the toxin were analyzed by SDS-PAGE. Isogenic mice were immunized with either the native or the irradiated toxin. The circulating antibodies were isotyped and titrated by ELISA. According to our data, irradiation promoted structural modifications in the toxin characterized by higher molecular weight forms of proteins (aggregates and oligomers). The results also indicated that irradiated toxins were immunogenic and antibodies elicited by them were able to recognize the native toxin in ELISA. These findings suggest that irradiation of toxic proteins can promote significant modifications in their structures; however they still retain many of the original antigenic and immunological properties of native proteins. Also, our data indicate that irradiated proteins induce higher titers of IgG2a and IgG2b, suggesting that Th1 cells are predominantly involved in the immune response. (author)

  6. Effects of 60Co γ-ray irradiation on growth characters of Chamaecrista seeds

    International Nuclear Information System (INIS)

    The seeds of 5 Chamaecrista varieties were irradiated by 60Co γ-ray of 300 Gy and the effects on M1 generation were studied. The results showed that the emergence of 5 Chamaecrista varieties decreased in some degree ; the stem-length of variety 34721 was significantly decreased, the stem-diameter of variety 2219 and the leaf-width of variety 2219 were significantly increased. The number of branch, leaf and leaf-length had no significant difference, but there were many black blots in the leaf during early period; the dry-weight of aerial part changed in two directions, either increased (92985) or decreased (34721). The total N of variety 2217 were significantly increased and the total P of variety 92985 were significantly decreased. The squaring stage, initiate flower stage, full bloom stage, pod-bearing stage, peak pod setting stage and maturing stage had changed in two direction, either early, or late

  7. Evaluation of myotoxic activity of Bothropstoxin-1 irradiated with 60Co gamma rays

    International Nuclear Information System (INIS)

    Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules and has been proven to be a powerful tool to attenuate snake venoms toxicity without affecting their immunogenic properties. Snake venoms and their isolated toxins showed structural modifications after gamma radiation process, in aqueous solution, mainly by water radiolysis sub products. Free radical scavengers, such as NaNO3 and t-butanol, present selective effects on specific radical from water radiolysis. The NaNO3 has affinity by aqueous electron, while the t-butanol has affinity by hydroxyl radical. At the present work, we have investigated the myotoxic activity of Bothropstoxin-1 (BTHX-1), a K49 phospholipase, present in Bothrops jararacussu crude venom, before and after irradiation process, with or without scavenger substances presence. BTHX-1 was irradiated with 2 kGy of 60Co gamma rays, in aqueous solution and in the presence of oxygen. BALB/c mice were inoculated with either native or irradiated toxin, with or without scavenger substances. After 3 hours, blood samples were collected and the myotoxic activity was evaluated by LDH (lactate dehydrogenase) release. The muscular tissue damage was directly related to the LDH amounts released. Irradiated Bothropstoxin-1, with or without NaNO3 substance, caused less damage than their native counterpart. But irradiated toxin, in the presence of t-butanol, was so myotoxic as the native BTHX-1. These results indicate that irradiation of toxic proteins can promote significant modifications on their structures, but still retaining many of the original biological properties of their native counterparts. Additionally, some scavengers substances can change these gamma radiation effects. (author)

  8. Effect of 60Co gamma-ray irradiation on dilute aqueous solutions of surfactants

    International Nuclear Information System (INIS)

    Present work deals with the effects of gamma irradiation from 60Co γ-ray source upon aqueous solutions of three kinds of surfactants. When dilute aqueous solutions of sodium dodecyl sulfate (SDS, anionic), cethyl trimethyl ammonium chloride (CTAC, cationic), and polyoxyethylene lauryl ether (POE, non-ionic) were irradiated with γ-rays at a room temperature, the residual concentration, products, surface tension, and forming power were examined by colorimetric method, IR spectrophotometric method, gaschromatography, Ross-Miles method, and Traube's stalagmometer etc. These surfactants were decomposed by the irradiation and thus the surface tension increased and the forming power, on the contrary, decreased with dose. Radiation chemical yields (G-value) of the degradation were about 1 for the solutions of SDS and CTAC, and about 0.3 for the POE solution. From the experimental results, it was found that following chemical reactions seem to occur followed by the radiolysis of water: a) bond cleavage of ester for SDS, of CN for CTAC, and of oxyethylene for POE, b) hydrogen abstraction from the surfactants, c) production of CO bond in the presence of dissolved oxygen. (auth.)

  9. 60Co irradiation for sterilization of veterinary mastitis products containing antibiotics and steroids

    International Nuclear Information System (INIS)

    Effects of 60Co irradiation for sterilization of veterinary mastitis products were evaluated. The mastitis products which were examined contained various combinations of antibiotics and steroids suspended in peanut oil vehicle. Bioburden data indicated that the unirradiated products were only occasionally contaminated with microorganisms. The D-values of the nonsterile product and environmental isolates were 0.028, 0.15, 0.017, and 0.018 Mrads for Aspergillus fumigatus, Penicillium oxalicum, Pseudomonas aeruginosa, and Pseudomonas maltophilia, respectively. The D-value of the biological indicator organism, Bacillus pumilus spores, in the vehicle was 0.27 Mrads. Thus, an irradiation dose of 1.6 Mrads would be sufficient to achieve six log cycles of destruction of the biological indicator organism. The minimum absorbed irradiation dose of 2.5 Mrads preferred by many countries for sterilization would achieve 9.3 log cycle destruction of the indicator organism and guarantee a probability of 1 x 10-15 assurance for the most radio-resistant product isolate, Penicillium oxalicum. In order to examine short and long term chemical stabilities of active components, stability indicating high-performance liquid chromatographic (HPLC) methods for the determination of several of the antibiotics and steroids were developed. The rates of degradation and radiolytic degradation schemes for the majority of the compounds were elucidated. (author)

  10. Chemical changes in the chloroform-paraffin system irradiated by 60Co gamma-rays, 1

    International Nuclear Information System (INIS)

    It has been reported that the chloroform-paraffin-dye system have excellent sensitivity for radiation as a solid chemical dosimeter or a phantom. However, the chemical changes in the irradiated system are not examined in detail. In the present study, the effect of paraffin on changes in the above system of a liquid state irradiated by 60Co γ-rays was examined by using various normal paraffin, and the other variable factors on the changes were done. When the chloroform solution and the solution containing 25 per cent of paraffin by volume with 5.0 x 15-5 mol/liter of Methyl Yellow as a dye were irradiated by 2000 R, G values for the formation of hydrogen chloride in the both solutions were 8.4 and 10.8, respectively, and were little affected by the kind of those, from C6 (hexane) to C36 (hexatria-contane). These results suggest that chlorine radical formed by radiolysis of chloroform may react with hydrogen atom from paraffin, thereby increasing the amount of hydrogen chloride. Presence of oxygen increased G value of the chloroform solution from 7.6 to 8.4, but did little that of the solution containing paraffin. (author)

  11. Biochemical and pharmacological characterization of irradiated crotamine by gamma rays of 60Co

    International Nuclear Information System (INIS)

    The serum production in Brazil, the only effective treatment in cases of snakebites, uses horses that although large size, have reduced l lifespan compared with horses not immunized. Ionizing radiation has been shown as an excellent tool in reducing the toxicity of venoms and toxins isolated, and promote the achievement of better immunogens for serum production, and contributing to the welfare of serum-producing animals. It is known, however, that the effects of ionizing radiation on protein are characterized by various chemical modifications, such as fragmentation, cross-linking due to aggregation and oxidation products generated by water radiolysis. However, the action of gamma radiation on toxins is not yet fully understood structurally and pharmacologically, a fact that prevents the application of this methodology in the serum production process. So we proposed in this paper the characterization of crotamine, an important protein from the venom of Crotalus durissus terrificus species, irradiated with 60Co gamma rays. After isolating the toxin by chromatographic techniques and testing to prove the obtaining of pure crotamine, it was irradiated with gamma rays and subjected to structural analysis, Fluorescence and Circular Dichroism. Using high hydrostatic pressure tests were also conducted in order to verify that the conformational changes caused by radiation suffer modifications under high pressures. From the pharmacological point of view, muscle contraction tests were conducted with the objective of limiting the action of crotamine in smooth muscle as well as the change in the action of toxin caused structural changes to the front. Analysis of Circular Dichroism and Fluorescence showed changes in structural conformation of crotamine when subjected to gamma radiation and that such changes possibly occurring in the secondary and tertiary structure of the protein. The observed in pharmacological tests showed that the irradiated crotamine was less effective in

  12. Perna perna (LINNAEUS, 1758) mussels irradiated by {sup 60}CO gamma rays cytotoxicity evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Martini, Gisela A.; Pusceddu, Fabio H.; Rogero, Sizue O.; Rogero, Jose Roberto, E-mail: gisela.martini@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    The aim of the present work was the study of ionizing radiation effects on aquatic biota regarding the location of nuclear facilities nearby coastal areas assuming the risk of leaks and nuclear accidents. Bivalve mollusks have been widely used in the monitoring of aquatic environment studies mainly for their sessile habit and pollutants bioconcentration ability. So marine mussel Perna perna (Bivalvia: Mytilidae) was used as organism test in this study. The study of radioactive toxicity was performed by cytotoxicity test exposing the organisms to 11Gy gamma radiation dose. After radiation the neutral red retention assay evaluated the lysosomal membrane integrity in the mussel hemocytes. 50% lethal dose assay (LD50) of gamma radiation on Perna perna mussels was carried out by exposure the organisms to {sup 60}Co gamma rays at doses ranging from 0 to 3000 Gy. The result of gamma radiation LD50 for these mussels was 1068 Gy and the neutral red retention time of irradiated organisms was about 47% lower than the control, non irradiated organisms. With the obtained results is expected to contribute in the study to identify the range of ionizing radiation doses which can cause toxic effects in marine invertebrates. (author)

  13. Biochemical and pharmacological characterization of irradiated crotamine by gamma rays of {sup 60}Co; Caracterizacao bioquimica e farmacologica da crotamina irradiada por raios gama de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Karina Corleto

    2014-07-01

    The serum production in Brazil, the only effective treatment in cases of snakebites, uses horses that although large size, have reduced l lifespan compared with horses not immunized. Ionizing radiation has been shown as an excellent tool in reducing the toxicity of venoms and toxins isolated, and promote the achievement of better immunogens for serum production, and contributing to the welfare of serum-producing animals. It is known, however, that the effects of ionizing radiation on protein are characterized by various chemical modifications, such as fragmentation, cross-linking due to aggregation and oxidation products generated by water radiolysis. However, the action of gamma radiation on toxins is not yet fully understood structurally and pharmacologically, a fact that prevents the application of this methodology in the serum production process. So we proposed in this paper the characterization of crotamine, an important protein from the venom of Crotalus durissus terrificus species, irradiated with {sup 60}Co gamma rays. After isolating the toxin by chromatographic techniques and testing to prove the obtaining of pure crotamine, it was irradiated with gamma rays and subjected to structural analysis, Fluorescence and Circular Dichroism. Using high hydrostatic pressure tests were also conducted in order to verify that the conformational changes caused by radiation suffer modifications under high pressures. From the pharmacological point of view, muscle contraction tests were conducted with the objective of limiting the action of crotamine in smooth muscle as well as the change in the action of toxin caused structural changes to the front. Analysis of Circular Dichroism and Fluorescence showed changes in structural conformation of crotamine when subjected to gamma radiation and that such changes possibly occurring in the secondary and tertiary structure of the protein. The observed in pharmacological tests showed that the irradiated crotamine was less effective

  14. Toxoplasma gondii vs ionizing radiation: intestinal immunity induced in C57bl/6j mice by irradiated tachyzoites

    International Nuclear Information System (INIS)

    We study the oral route for the development of a vaccine for toxoplasmosis, using parasites irradiated with 60 Cobalt, as an alternative for vaccine development to this worldwide parasitic infection. We evaluated the development of immunity at serum or mucosal levels, and their efficiency in protect the mice against challenge with oral cysts of the Me-49 strain. C57Bl/6j isogenic mice were immunized by oral route with 107 255 Gy irradiated tachyzoites from RH strain, at several protocols using milk as anti-peptic adjuvant and alum hydroxide as antacid. The preparations of irradiated tachyzoites induced production of serum IgG and IgA in immunized mice, as determined by ELISA, with IgG2a as the dominant subclass, similar to chronic infection. Their use with adjuvant allowed the excretion of significant amounts of IgA in stools also IgG, despite a lesser extent. There are suggestion of tolerance induction at mucosal level, with lower antigen induced proliferation and lower in vitro antibody production by spleen and gut lymphocytes, with the latter doses, specially when milk was used as adjuvant. All oral preparations induced some quantitative protection against challenge, which was similar to the parenteral route only isolated alum hydroxide was used as adjuvant. All these data support the possibility of the development of an oral vaccine against toxoplasmosis, using irradiated tachyzoites, which would be possible tool in near future for use in field baits, for immunizing either domestic or wild felines. (author)

  15. Detection and thermoluminescence of coriander (Coriandrum sativum L.) irradiated with 60Co

    International Nuclear Information System (INIS)

    The fresh and dried coriander (Coriandrum sativum L.) is a spice of high commercial value as food for consumption in Mexico. Their hygienic quality is often poor due to some factors: contaminants present in irrigation water and the cultivation atmosphere, harvesting and post harvest handling, mainly. The process by radiation is an alternative to achieve the sterilization to adequate dose for the hygienic quality for its consumption. However, irradiation also involves food detection exposed to radiation. This paper presents detected results on the dried coriander that was obtained from fresh samples and thermoluminescent properties such as glow curves structure from low doses (0.5 Gy) to high (15 kGy), the dose response, thermoluminescent signal decay, in order to determine the loss of stability during the storage of the poly mineral fraction. We obtained the inorganic fraction separation of the organic part of particle sizes of 10 μm by the Zimmerman method. The samples were exposed at two dose ranges; 0.5-400 Gy and 0.5-15 kGy of gamma radiation with 60Co. The limit of thermoluminescent detection of the irradiated coriander samples was from 1 Gy. The glow curves were at a broad band of 35-400 C, with a maximum thermoluminescence around 182-196 C, and run at 164 C for high doses of 15 kGy. The range of linear response to dose was 4-25 Gy, whereas higher doses than the kGy order the response increase with the dose. The thermoluminescent properties of the coriander poly mineral fraction, show it can be used to identify irradiated food at gamma doses relatively low and even in doses of commercial interest due to the high stability of thermoluminescent signals. (Author)

  16. Effects on agronomic traits of M1 by pollen of upland cotton irradiated by 60Co-γ ray

    International Nuclear Information System (INIS)

    [Objective] The aim was to provide reference for research on radiation and breeding of cotton pollen through irradiating common ripe pollen grain of upland cotton by 60Co-? Ray of varied doses. [Method] Ripe pollen grains of upland cotton were irradiated by 60Co-γ Ray with doses of 5, 10, 15, and 20 Gy, respectively, to learn radiation effect and select appropriate dose. [Result] Most properties of M1 obviously showed variation when dose was over 10 Gy; vitality, growth, and fertility were greatly inhibited when dose was 15 Gy which was almost semi-lethal concentration, and variation species were richest at the same time, which provided materials for practical breeding. [Conclusion] 60Co-γ Ray of 15 Gy was more suitable for inducing research for ripe pollen grains of upland cotton

  17. Detection and thermoluminescence of coriander (Coriandrum sativum L.) irradiated with {sup 60}Co; Deteccion y termoluminiscencia del cilantro (Coriandrum sativum L.) irradiado con {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Ruiz G, B. [Departamento de Agricultura y Ganaderia, Universidad de Sonora, 083190 Hermosillo, Sonora (Mexico); Cruz Z, E. [Unidad de Irradiacion y Seguridad Radiologica, Instituto de Ciencias Nucleares, UNAM, A. P. 70-543, 04510 Mexico D. F. (Mexico); Barboza F, M. [Centro de Investigacion en Fisica, Universidad de Sonora, A. P. 5-088, 083190 Hermosillo, Sonora (Mexico)

    2009-10-15

    The fresh and dried coriander (Coriandrum sativum L.) is a spice of high commercial value as food for consumption in Mexico. Their hygienic quality is often poor due to some factors: contaminants present in irrigation water and the cultivation atmosphere, harvesting and post harvest handling, mainly. The process by radiation is an alternative to achieve the sterilization to adequate dose for the hygienic quality for its consumption. However, irradiation also involves food detection exposed to radiation. This paper presents detected results on the dried coriander that was obtained from fresh samples and thermoluminescent properties such as glow curves structure from low doses (0.5 Gy) to high (15 kGy), the dose response, thermoluminescent signal decay, in order to determine the loss of stability during the storage of the poly mineral fraction. We obtained the inorganic fraction separation of the organic part of particle sizes of 10 {mu}m by the Zimmerman method. The samples were exposed at two dose ranges; 0.5-400 Gy and 0.5-15 kGy of gamma radiation with {sup 60}Co. The limit of thermoluminescent detection of the irradiated coriander samples was from 1 Gy. The glow curves were at a broad band of 35-400 C, with a maximum thermoluminescence around 182-196 C, and run at 164 C for high doses of 15 kGy. The range of linear response to dose was 4-25 Gy, whereas higher doses than the kGy order the response increase with the dose. The thermoluminescent properties of the coriander poly mineral fraction, show it can be used to identify irradiated food at gamma doses relatively low and even in doses of commercial interest due to the high stability of thermoluminescent signals. (Author)

  18. Efficacy of a 60Co irradiated vaccine for experimentally infected calves with dictyocaulus viviparus

    International Nuclear Information System (INIS)

    Dictyocaulus viviparus larvae in non-infected stage (L.1) were cultured in vitro to their infective stage (L.3) and were irradiated with 40 Krad from 60Co and used as a vaccine. The oral experimental vaccine dose was 1000 L.3/animal. Three groups were formed with 8 calves in each one: group No. 1 and group No. 2 were vaccinated at 10 weeks of age. Four weeks later group No. 1 was infected with non-irradiated 60 L.3/Kg. of animal weight 'challenge dose'. After four weeks of post-vaccination group No. 2 was revaccinated and 4 weeks later it was infected with the 'challenge dose' as the previous group. Eight calves constituted group No. 3, four in each group; they were infected with only the challenge dose respectively. Information data on respiratory and cardiac frequency, temperature, weight, and L.1 count/g. of feces were obtained from each animal three times a week. After five weeks of post-challenge all animals were slaughtered to observe anatomopathologic lessions in the heart and lungs; the number of adult Dictyocaulus viviparus present in the respiratory tract were search 't'. Student test was used for the statistical analysis. The weight increment difference between animals of group No. 1 related control group was 7Kg.; and 11.25 Kg/animal in group No. 2 respectively. The percentage of protection confered by the vaccine to the subjects in group No. 1 relative to the respectivecontrol group, was 83.2; that for the group No. 2 was 88.5 per cent. Post-morten examination revealed severe anatomopathologic lessions in the control groups; only few lessions were observed in group No. 1 and practically none in group No. 2

  19. Viabilidade e micoflora de sementes de amendoim irradiadas com cobalto (60Co Viability and mycoflora of irradiated seeds of peanut with cobalt (60Co

    Directory of Open Access Journals (Sweden)

    Niédja M. C. Alves

    2011-03-01

    Full Text Available Estudou-se o efeito de diferentes doses da irradiação gama na germinação e percentagem de infecção fúngica em sementes de amendoim cultivar BR1. Inicialmente, as sementes foram avaliadas quanto à micoflora, utilizando-se o método de papel de filtro umedecido, a germinação em substrato de papel germitest e determinação do teor de umidade em estufa, a 105 ± 2 ºC. Posteriormente, as sementes foram submetidas à irradiação com uma fonte de 60Co, tipo gammacell, onde se estudou o efeito de 8 doses de irradiação na micoflora e germinação das sementes acondicionadas em embalagem de PET e polietileno trançado durante 60 dias de armazenamento, sem controle de temperatura e umidade relativa do ar. Os fatores estudados (doses em kGy, embalagens e tempo de armazenamento foram analisados em um delineamento inteiramente casualizado, dispostos em fatorial. Com base nos resultados, observou-se que a irradiação gama afetou negativamente a germinação das sementes de amendoim e que doses acima de 3,0 kGy comprometem a germinação das sementes. A radiação a partir da dose 1,5 kGy eliminou os fungos Aspergillus niger e, Penicillium a partir da dose de 2,5 kGy.The effect of different doses of the gamma irradiation was studied on germination and percentage of fungal infection in peanut seeds cultivar BR1. Initially the seeds were evaluated for the mycoflora using the method of humidified filter paper, the germination in paper substratum germitest and determination of the moisture content in oven at 105 ± 2 ºC. Later on the seeds were submitted to irradiation with a source of 60Co, type gammacell, where the effect was studied of 8 doses of irradiation in the mycoflora and germination of the seeds conditioned in PET packing and polyethylene tressed during 60 days of storage, without control of temperature and relative humidity of the air. The studied factors (doses in kGy, packing and time of storage were analyzed in a completely

  20. 60CoIrradiation Affects the Enzymatic Antioxidant System of the Citrus Red Mite Panonychus citri (Acari: Tetranychidae

    Directory of Open Access Journals (Sweden)

    Ke Zhang

    2014-05-01

    Full Text Available Radio-(60Co, which emits γ rays, has been used worldwide in pest control. The aim of the present study was to analyze the effect of effective-low-power 60Coirradiation on the enzymatic antioxidant system of the citrus red mite Panonychus citri. One day old female adults were exposed to 0.4 kGy 60Coirradiation and on the, 6th h, 1st day, 2nd day, and 5th day post treatment, the mites were euthanized for biochemical analysis. The activity of superoxide dismutase (SOD, catalase (CAT, peroxidase (POD, phenoloxidase (PO and acetylocholinesterase (AchE were investigated. POD and CAT activities, as well as SOD were higher in the irradiated mites. We found that exposure to 60Co-γ radiation resulted in increased activities of SOD, CAT, POD and decreased AchE activity. When the recovery time lasted till the 5th day, the activities of POD and PO were significantly lower than the control, whereas the SOD, CAT and AchE activities returned to control levels. Cells possess protein repair pathways to rescue oxidized proteins and restore their functions, but if these repair processes fail, oxidized proteins may become cytotoxic. Our results confirm the hypothesis that low dosages of 60Coirradiation increase the level of oxidative stress in P. citri adults in a short time, causing meanwhile damage and sterility. The results of this study also indicate that stress caused by exposure to irradiation could inhibit the cholinergic system in P. citri.

  1. (60)Coirradiation affects the enzymatic antioxidant system of the citrus red mite Panonychus citri (Acari: Tetranychidae).

    Science.gov (United States)

    Zhang, Ke; Li, Zhibin; Zhu, Shaowen; Weng, Qunfang

    2014-01-01

    Radio-(60Co), which emits γ rays, has been used worldwide in pest control. The aim of the present study was to analyze the effect of effective-low-power 60Coirradiation on the enzymatic antioxidant system of the citrus red mite Panonychus citri. One day old female adults were exposed to 0.4 kGy 60Coirradiation and on the, 6th h, 1st day, 2nd day, and 5th day post treatment, the mites were euthanized for biochemical analysis. The activity of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), phenoloxidase (PO) and acetylocholinesterase (AchE) were investigated. POD and CAT activities, as well as SOD were higher in the irradiated mites. We found that exposure to 60Co-γ radiation resulted in increased activities of SOD, CAT, POD and decreased AchE activity. When the recovery time lasted till the 5th day, the activities of POD and PO were significantly lower than the control, whereas the SOD, CAT and AchE activities returned to control levels. Cells possess protein repair pathways to rescue oxidized proteins and restore their functions, but if these repair processes fail, oxidized proteins may become cytotoxic. Our results confirm the hypothesis that low dosages of 60Coirradiation increase the level of oxidative stress in P. citri adults in a short time, causing meanwhile damage and sterility. The results of this study also indicate that stress caused by exposure to irradiation could inhibit the cholinergic system in P. citri. PMID:24853454

  2. Influence of 60Co γ irradiation on fertility of Japanese pine sawyer beetle Monochamus alternatus hope (Coleoptera: Cerambycidae)

    International Nuclear Information System (INIS)

    The fertility of the Japanese pine sawyer beetle Monochamus alternatus (Coleoptera: Cerambycidae) irradiated with 60Co γ-rays was remarkable reduced at the doses of 30Gy, 35Gy and 40Gy, especially at 40Gy. When the non-irradiated females were coupled with the irradiated males first, and then coupled with non-irradiated males, the hatchability and the fertility had little higher but lower than the control. It explained that radiation has certain influence to the female gonad. It also has difference between the hatching rate and the amount of eggs in different match. (authors)

  3. Expression of FHIT in AHH-1 cells irradiated by 60Co γ-ray and bystander effect cells

    International Nuclear Information System (INIS)

    Objective: To investigate the expression of FHIT gene in the 60Co gamma-ray irradiated human lymphocytoblast (AHH-1) cell and the bystander effect cell, and to explore the function of FHIT gene in the bystander effect of ionizing radiation. Method: Preparation of bystander effect cell model: after inadiated with different dose of 60Co gamma-ray (0, 2, 5 Gy), the directly irradiated AHH-1 cells were collected immediately by centrifugation and co-cultivated with non-irradiated cells in Transwell, forming the bystander effect group P1. In addition, some culture media supematant of directly irradiated cells were transferred to the non- irradiated cells culture medium, forming the group P2. Then cells were collected at 0, 6, 12, and 24 h after irradiation and the total RNA and protein were extracted. RT-PCR and Western blot were performed to determine the FHIT mRNA and protein level, respectively. Flow cytometry assay and cell counting were conducted to detect the alteration of cell cycle and cell proliferation, respectively at 0, 24 h after irradiation. Results: The mRNA level of FHIT gene among control cells, directly irradiated cells and bystander cells showed no obvious difference, while the FHIT protein level of the directly irradiated cells and bystander cells was significantly down-regulated compared with the control cells (F=102.45, P2 phase arrest and obviously inhibited the proliferation ability. Conclusions: 2 and 5 Gy of 60Co γ-ray irradiated AHH-1 cells can result in down regulation of the FHIT protein expression, which suggests that FHIT gene is involved in the process of bystander effect induced by irradiation. (authors)

  4. Effect of irradiation using 60Co on Trichinella spiralis larvae and on the eggs and protoscolices of Echinococcus granulosus

    International Nuclear Information System (INIS)

    The effect of irradiation using 60Co on Trichinella spiralis larvae and on the eggs and protoscolices of Echinococcus granulosus was studied. A dose of 30 Gy had no effect on T. spiralis larvae and on the eggs and protoscolices of E. granulosus. Irradiation of free T. spiralis larvae from muscle in normal saline exposed to 90 Gy and higher doses produced loss of infection in mice. There was reduction in the infectivity of eggs and protoscolices of E. granulosus by irradiation at a dose of 120 Gy, while there was complete loss of activity at a dose of 500 Gy. (author). 5 refs, 2 figs

  5. Effect of 60Co γ-rays irradiation pretreatment on callus inductivity and differentiation of strawberry anther

    International Nuclear Information System (INIS)

    Strawberry buds, in which a majority of pollen had single nuclear keeping to the side, were pretreated by 60Co γ-rays irradiation at different doses. The irradiation effect on the callus inductivity and plant differentiation rate of strawberry anther were studied. The results indicated that the pretreatment by 60Co γ-rays could increase the anther callus inductivity and plant differentiation rate significantly, and 20 Gy was the best dose. The callus inductivity of Akihime and Toyonoka increased by 39.09% and 35.68%, respectively, compared with the no-pretreatment materials. On the same media, the plant differentiation rate of Akihime and Toyonoka were also increased by 6.67%-6.72% and 4.73%-6.45%, respectively, compared with the two varieties of no-pretreatment. (authors)

  6. Grafting of synthetic polyelectrolyte onto polymer surfaces--comparison of glow discharge and 60Co-gamma-irradiation method

    International Nuclear Information System (INIS)

    Water soluble polyelectrolyte synthesized from natural rubber contains sulfamate and carboxylate groups similar to that of heparin. It is observed that synthetic heparinoid polyelectrolyte is capable of inhibiting blood coagulation. In the present study, we attempted to graft the same onto polystyrene and polymethylmethacrylate surfaces using glow discharge technique and 60Co-gamma-irradiation method, and the surfaces were compared with respect to water contact angle and platelet adhesion parameters. Heparinized surfaces are also evaluated for relative comparison

  7. Manufacture of Single 60Co Source Irradiation Facility and the Measurement of Reference Radiation Filed

    Institute of Scientific and Technical Information of China (English)

    GAO; Fei; WANG; Hong-yu; NI; Ning; ZHANG; Li; HOU; Jin-bing; SONG; Ming-zhe

    2012-01-01

    <正>Reference radiation filed produced by isotope source is necessary for calibration of radiation dose meter. 60Co single source radiation facility is an important method to produce reference radiation. Collimation design of the facility is good for characteristics of the field, but there are scattered photon influences however. Scattered radiation is mainly come from collimation, source shield, floor, walls and

  8. Study on the down-regulation of PKCα by tamoxifen combined with 60Co γ-irradiation in glioma cells

    International Nuclear Information System (INIS)

    The mechanism of radio-sensitizing effect of tamoxifen on the influence of PKCα expression has been explored by tamoxifen combined with 60Co γ-irradiation in human glioma cells. The protein expression of PKCα and CyclinD1 were detected by using Western-Blot. The level of DNA strand breaks was detected by the alkaline single-cell gel electrophoresis assay. The results demonstrated that the combination of tamoxifen and 60Co γ-irradiation could reduce expression of PKCα and CyclinD1. And it also enhanced the radiation-induced DNA damage and attenuated the repair of damaged DNA. The above results reveal that the combination of tamoxifen and 60Co γ-irradiation can enhance the downregulation of the expression of PKCα and the induction in the low expression of CyclinD1 as well at the delay of DNA repair. All these may be related to the impairment of the radioresistance of SHG-44 cells by tamoxifen. (authors)

  9. Effects of accumulated 60Co γ-ray irradiation on the small molecular metabolites in rats urine

    International Nuclear Information System (INIS)

    Objective: To explore the effects of accumulated 60Co γ-ray irradiation on small molecular metabolites in rats urine. Methods: Ten healthy male SD rats were irradiated by 60Co γ-rays in 5 days and the accumulated doses were 0, 0.2, 0.4, 0.6, 0.8, 1.0 Gy, respectively. The metabolites in urine of different groups were measured with 1H-NMR combined with principal components analysis (PCA) and partial least square discriminate analysis (PLS-DA). Results: The metabolites in rat urine were obviously changed after irradiation. Compared with control group, the amount of acetoacetate decreased after irradiation (t=29.7-30.7, P<0.05), but its relative level was stable when the dose increased (P>0.05). Meanwhile,the relative level of hippuric acid increased (t=4.4-21.6, P<0.05) especially when the accumulated dose was higher than 1 Gy (t=21.6, P<0.05). The relative level of proline, taurine and trimethylamine-N-oxide increased after irradiation with the same trend(t=3.5-13.4, 4.7-11.5, 2.9-12.7, P<0.05). Conclusions: The acetoacetate, hippuric acid, proline, taurine, and trimethylamine-N-oxide may be applicable for biomarkers of accumulative irradiation on rat. (authors)

  10. Effects of 60Co γ-ray irradiation on expression of surface antigens in endothelial cells of human umbilical veins

    International Nuclear Information System (INIS)

    Culture of endothelial cells of human umbilical veins and avidin-biotin peroxidase complex (ABC) immunochemical technique were used in the experiment to detect the surface antigens in endothelial cells. Endothelial cells separated from five umbilical cords in original culture were divided into two groups, irradiated and non-irradiated. The cells were irradiated with 15 Gy of 60Co γ-rays at dose rates of 21.78 cGy/min. Then antigens RBC A, HLA-ABC, HLA-DR, CD4 and CD8 were assayed for both groups by the method of ABC. The results showed that the values of integrated optical density (IOD) for the surface antigens in the irradiated cells were lower than those in the non-irradiated cells with the difference in antigen expression in endothelial cells being significant (P<0.05) between the two groups

  11. 60Co-irradiation as an alternate method for sterilization of penicillin G, neomycin, novobiocin, and dihydrostreptomycin

    International Nuclear Information System (INIS)

    The effects of the use of 60Co-irradiation to sterilize antibiotics were evaluated. The antibiotic powders were only occasionally contaminated with microorganisms. The D-values of the products and environmental isolates were 0.028, 0.027, 0.015, 0.046, 0.15, 0.018, and 0.19 Mrads for Aspergillus species (UC 7297, 7298), A. fumigatus (UC 7299), Rhodotorula species (UC 7300), Penicillium oxalicum (UC 7269), Pseudomonas maltophilia (UC 6855), and a biological indicator microorganism, Bacillus pumilus spores (ATCC 27142). An irradiation dose of 1.14 Mrads, therefore, was sufficient to achieve a six-log cycle destruction of B. pumilus spores. Based on the bioburden data, a minimum irradiation dose of 1.05 Mrads was calculated to be sufficient to obtain a 10(-6) probability of sterilizing the most radioresistant isolate, Pen. oxalicum. To determine the radiolytic degradation scheme and the stability of the antibiotics following irradiation, high-performance liquid chromatographic (HPLC) methods were developed. The resulting rates of degradation for the antibiotics were 0.6, 1.2, 2.3, and 0.95%/Mrad for penicillin G, neomycin, novobiocin, and dihydrostreptomycin, respectively. Furthermore, radiolytic degradation pathways for the antibiotics were identified and found to be similar to those commonly encountered when antibiotics are subjected to acidic, basic, hydrolytic, or oxidative treatments. No radiolytic compounds unique to 60Co-irradiation were found

  12. Suppression by ellagic acid of 60Co-irradiation-induced lipid peroxidation in placenta and fetus of rats

    International Nuclear Information System (INIS)

    The effect of ellagic acid, a component of Eucalyptus maculata, on lipid peroxidation was examined in placenta and fetus of pregnant rats irradiated with 60Co. The increase in lipid peroxide levels by the irradiation of the placenta and fetus brain as well as those of the serum and organs of mother was suppressed by treatment of the mother rats with ellagic acid. This suppressing effect found in placenta and fetus was significantly correlated with that found in mother rats. Moreover, ellagic acid suppressed the morphological changes such as degeneration in the endothelial cells of placenta and liver cells of fetus caused by the irradiation and improved the survival rate after the irradiation. These suppressing effects of ellagic acid were approximately the same as those of α-tocopherol. (author)

  13. Morphological and histological studies on freshwater prawn Macrobrachium rosenbergii (de man) irradiated with {sup 60}Co gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Stalin, A.; Broos, K.V. [P.G. and Research Department of Zoology, Jamal Mohamed College (Autonomous), Tiruchirappalli 620020, Tamil Nadu (India); Sadiq Bukhari, A., E-mail: abjmc@yahoo.in [P.G. and Research Department of Zoology, Jamal Mohamed College (Autonomous), Tiruchirappalli 620020, Tamil Nadu (India); Syed Mohamed, H.E. [P.G. and Research Department of Zoology, Jamal Mohamed College (Autonomous), Tiruchirappalli 620020, Tamil Nadu (India); Singhal, R.K. [Analytical Spectroscopy Section, Analytical Chemistry Division, Bhabha Atomic Research Center, Trombay, Mumbai 4000085 (India); Venu-Babu, P. [Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center, Trombay, Mumbai 4000085 (India)

    2013-11-15

    Graphical abstract: -- Highlights: •Analyzing the impact of {sup 60}Co gamma radiation (low doses) in M. rosenbergii. •LD{sub 50} identified in 30 Gy; Irradiated exhibits morphological changes than control. •Significantly decreased HSI and histological changes in irradiated groups. •Aberration severities increased as increased of dose level. •Biomonitoring tool in determining low dose radiation pollution in aquatic ecosystem. -- Abstract: This study was framed to investigate the {sup 60}Co gamma radiation induced morphological and histological variations in freshwater prawn Macrobrachium rosenbergii. The LD{sub 50} value of {sup 60}Co gamma irradiated M. rosenbergii observed (by probit analysis) at 30 Gy. Prawns were irradiated to four different dose levels (3 mGy, 30 mGy, 300 mGy and 3000 mGy) using Theratron Phoenix TeleCobalt Unit [P-33] and one control group (without irradiation) maintained separately. Irradiated groups exhibited several morphological variations such as discoloration; damaged rostrum; opaque coloration in cephalothorax; black bands and dot formation in abdomen; deformed uropods and telson in tail regions when compared with control group. The Hepato Somatic Index reflected the severity of radiation on hepatopancreas. Histological variations in gills, hepatopancreas and muscles of irradiated groups were observed. In gills, structural changes such as swollen and fused lamellae, abnormal gill tips, hyperplasic, necrotic and clavate-globate lamellae were observed in gamma irradiated prawns. Accumulation of hemocytes in hemocoelic space, interstitial sinuses filled with abnormal infiltrated hemocytes, the tubular epithelium with ruptured basal laminae, abnormal and coagulated lumen, necrotic tubules, thickened basal laminae, tissue debris, necrotic hepatocytes were observed in irradiated prawn hepatopancreas. In muscle, shrinkage of muscular fiber and necrotic musculature were observed in irradiated prawns. These structural alterations of

  14. Morphological and histological studies on freshwater prawn Macrobrachium rosenbergii (de man) irradiated with (60)Co gamma radiation.

    Science.gov (United States)

    Stalin, A; Broos, K V; Sadiq Bukhari, A; Syed Mohamed, H E; Singhal, R K; Venu-Babu, P

    2013-11-15

    This study was framed to investigate the (60)Co gamma radiation induced morphological and histological variations in freshwater prawn Macrobrachium rosenbergii. The LD50 value of (60)Co gamma irradiated M. rosenbergii observed (by probit analysis) at 30 Gy. Prawns were irradiated to four different dose levels (3 mGy, 30 mGy, 300 mGy and 3,000 mGy) using Theratron Phoenix TeleCobalt Unit [P-33] and one control group (without irradiation) maintained separately. Irradiated groups exhibited several morphological variations such as discoloration; damaged rostrum; opaque coloration in cephalothorax; black bands and dot formation in abdomen; deformed uropods and telson in tail regions when compared with control group. The Hepato Somatic Index reflected the severity of radiation on hepatopancreas. Histological variations in gills, hepatopancreas and muscles of irradiated groups were observed. In gills, structural changes such as swollen and fused lamellae, abnormal gill tips, hyperplasic, necrotic and clavate-globate lamellae were observed in gamma irradiated prawns. Accumulation of hemocytes in hemocoelic space, interstitial sinuses filled with abnormal infiltrated hemocytes, the tubular epithelium with ruptured basal laminae, abnormal and coagulated lumen, necrotic tubules, thickened basal laminae, tissue debris, necrotic hepatocytes were observed in irradiated prawn hepatopancreas. In muscle, shrinkage of muscular fiber and necrotic musculature were observed in irradiated prawns. These structural alterations of the organs it is felt could affect the vital physiological functions such as respiration, osmotic and ionic regulation in gills and muscles; absorption, storage and secretion of the hepatopancreas which in turn could adversely affect the growth and survival of freshwater prawn M. rosenbergii.

  15. The sensitivity of the most common microorganisms of pig liquid manure to the effect of 60Co irradiation

    International Nuclear Information System (INIS)

    In model experiments the most common representatives of pathogens and facultative pathogens of pig liquid manure contaminating bacterial flora were examined for their sensitivity to different doses of 60Co, using liquid medium supplemented with 1% glucose, as well as Korthof-medium and different kinds of selective media. Erysipelothrix rhusiopathiea, Brucella suis, Mycoplasma hyorhinis and Pasteurella haemolytica proved to be the most senstive ones to irradiation, as they did not survive 100 krad dose. The highest resistances were observed in the cases of the following microorganisms: the spore containing Bacillus anthracis, killed by 700 krad irradiation dose, the saprophytic and pathogenic Mycobacteria and the spore containing Clostridium perfringens, killed by 1000-1500 krad irradiation. (author)

  16. Effect of combination of STAT3 RNAi and 60Co γ-irradiation on U251 cell proliferation

    International Nuclear Information System (INIS)

    Objective: To construct signal transduction and activators of transcription 3 (STAT3) small interference RNA (siRNA) expression vector and to study its effect on STAT3 expression and U251 cell line proliferation. Methods: STAT3 specific 19 bp oligonucleotides were designed and synthesized. These oligonucleotides were annealed to form the double strand DNA fragments and these fragments were cloned into Psilence2.1-U6-H1 vector. The recombinant of STAT3-siRNA expressing construction was confirmed by Hind III and BamH I double digestion and sequencing. The STAT3-siRNA was transfected into U251 cell. The inhibitory effect of STAT3-siRNA construction was tested by Western blot. Cellular proliferation activities were measured by tetrazolium bromide (MTT) colorimetry. Cloning efficiency and MTF were used to confirm the radiation dose. Results: STAT3-siRNA expression vector was successfully constructed, and it could effectively down-regulate the protein levels of STAT3 in transfected U251 cell line; and the radiation dose was confirmed to 2 Gy. U251 cells transfected with STAT3-siRNA expression vector showed lower cellular proliferation compared with non-transfected U251 cells (P60Co γ-irradiation showed lower cellular proliferation compared with non-irradiated U251 cells (P60Co γ irradiation can enhance the inhibitory efficiency. (authors)

  17. Effects of (60)Co gamma irradiation on behavior and gill histoarchitecture of giant fresh water prawn Macrobrachium rosenbergii (DE MAN).

    Science.gov (United States)

    Stalin, A; Broos, K V; Sadiq Bukhari, A; Syed Mohamed, H E; Singhal, R K; Venu-babu, P

    2013-06-01

    Present study was designed to observe the effects of (60)Co gamma radiation in behavioral and histological changes in the gills of giant fresh water prawn Macrobrachium rosenbergii. The adult prawns were irradiated with four different dose levels (3mGy, 30mGy, 300mGy and 3000mGy) and the control group (without irradiation) was maintained separately. Behavioral changes like hyperactivity, loss of balance, reduced swimming rate, slower rate of food intake and convulsions were observed in higher dose levels of 300mGy and 3000mGy. The histological alterations such as accumulated haemocytes in haemocoelic spaces, abnormal gill tips, lifted lamellar epithelium, swollen and fused lamellae, hyperplasic, necrotic, clavate-globate and complete disorganization of lamellae were observed in (60)Co gamma irradiated prawns. Significantly more considerable histological alterations were observed in the highest dose level of 3000mGy, but no mortality was evidenced. This study serves as biomonitoring tool to assess the radiation pollution in the aquatic environment.

  18. Humoral immune response against native or {sup 60}Co irradiated venom and mucus from stingray Paratrygon aiereba

    Energy Technology Data Exchange (ETDEWEB)

    Thomazi, Gabriela Ortega Coelho; Alves, Glaucie Jussilane; Aires, Raquel da Silva; Turibio, Thompson de Oliveira; Rocha, Andre Moreira; Spencer, Patrick Jack; Nascimento, Nanci do, E-mail: 0916@prof.itpacporto.com.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Seibert, Carla Simone, E-mail: carlaseibert@yahoo.com [Universidade Federal do Tocantins (UFT), Porto Nacional, TO (Brazil)

    2015-07-01

    Poisonings and traumas caused by poisonous freshwater fish such as rays are considered a major public health problem and draw attention because of accidents involving these animals cause serious local symptoms and are disabling, keeping the victim away from work. The therapy of these cases is based only on the symptoms of patients, which implies in its low efficiency, causing suffering for the victims. This study aims to evaluate and compare the humoral immune response in animals inoculated with native or {sup 60}Co irradiated Paratrygon aiereba venom and mucus. Ionizing radiation has proven to be an excellent tool to decrease the toxicity of venoms and isolated toxins. The mucus and venom samples of P. aiereba were irradiated using gamma rays from a {sup 60}Co source. Animals models were immunized with the native or irradiated mucus or venom. The assays were conducted to assess the production of antibodies by the immunized animals using enzyme immunoassay and western blotting. Preliminary results show the production of antibodies by the immunized animals. The resulting sera were also checked for antigenic cross- reactivity between venom and mucus, demonstrating the potential of mucus as an antigen for serum production for the specific treatment for accidents by stingrays. However, it is essential to carry out further tests in order to verify the neutralization of the toxin by antibodies formed by animals. (author)

  19. Study of the ionization of alkane-electron scavenger reactant mixtures irradiated by 60Co gamma rays

    International Nuclear Information System (INIS)

    This study deals with ionization of alkane-electron scavenger reactant mixtures, irradiated by 60Co γ-rays. It is shown that the extrapolated free-ion yields (extrapolated yield method) decrease with the reactant concentration. On the basis of ONSAGER model and theoretical treatment of MOZUMDER, the cross sections of epithermal electron attachment in hexane, cyclohexane, 2,2-dimethylbutane, cyclopentane, 2,2,4-trimethylpentane for CCl4, C7F14, C6H5Br, C6H5Cl, C6F14, (C6H5)2 are determined. A comparison between gas-phase and liquid-phase cross sections is established

  20. 60Co-γ-irradiation of dried DNA and isolated cell nuclei of chicken erythrocytes

    International Nuclear Information System (INIS)

    In this work low molecular products, which resulted from γ-irradiation of dried DNA, were isolated and quantitatively determined. Unchanged nucleic bases were released. The irradiation was successful in a vacuum as well as in oxygen. In the case of the drily irradiated DNA, the base release made up 30% of the total strand breaks. The release of DNA bases was also first studied in cell nuclei of eucaryotic cells, the erythrocyte nuclei of chicken blood. The second part of this work dealt with the isolation and identification of radiation induced changes in the 2-dioxyribose unit of DNA. In the third part it was investigated, whether as a result of γ-irradiation of DNA malonic dialdehyde was formed. It could be shown that neither malonic aldehyde nor basic propenal were formed, but instead products, which were still bound to the DNA and formed a chromophore with 2-thio barbituric acid. In this work the direct irradiation effect was investigated in DNA systems as well as in erythrocyte nuclei. By the isolation of low molecular irradiation products this work offers a contribution to the understanding of the irradiation chemistry in an eucaryotic cell, in which the direct effect on the DNA and the resulting radiation damage were given a deciding role for genetic information. (orig./MG)

  1. Cutaneous mitochondrial protection by topical treatment of herbal antioxodiants following 60 Co-gamma irradiation

    International Nuclear Information System (INIS)

    To find out the effective dose of herbal antioxidants to elevate the cutaneous lipid peroxidation (LP) at optimal level, gamma irradiation of cutaneous microsomal fraction was performed at different doses and 200 Gy was chosen. The present findings indicate that the failure of succinate in suppression of mitochondrial LP of irradiated rats might be due to diminishing of mitochondrial antioxidants which had been reduced by free radicals (generated by irradiation), since succinate suppressed mitochondrial LKP in antioxidant-treated rats. From these points of view, topical application of either curcumin or ellagic acid could be beneficial in prevention of radiation skin cancer

  2. Effect of 60Coirradiation on mating ability of males of Helicoverpa armigera

    International Nuclear Information System (INIS)

    The effects of γ-rays irradiation on the mating ability and flight capability of male cotton bollworm Helicoverpa armigera were studied in laboratory and field cages. The results showed that when the irradiation dose increased from 0Gy to 400Gy, the flight capability decreased: flight distance decreased from 69.65km to 38.30km; average flight distance decreased from 44.19km to 13.55km. The longevity of the irradiated males was significantly shorter than that of the wilds but there was no significant difference according to different doses. Egg hatchability was affected significantly by irradiation but there was no significant difference in eggs laid per female, fecundation eggs as increasing treated doses. When the ratio of sterilized males (treated dose 200 Gy) to fertile males was as 5:1 in field cages, the F1 hatchability was significantly decreased and the purpose of controlling cotton bollworm population could be effectively achieved. (authors)

  3. Effects of 60Coirradiation sterilization and influence on the quality of paprika powder

    International Nuclear Information System (INIS)

    The pollution of paprika powder was investigated and effects of gamma irradiation on the sterilization and quality were studied. The results showed that paprika powder was seriously polluted and gamma irradiation reduced significantly the total population of mesophilic bacteria, coliforms, lactobacillus, micrococcus, mould and spore. The mortality rate could reach 82%-94% at 2.62 kGy. There was no significant different between three kinds of packaging. The D10 va1ue of coliforms was 2.05-3.21 kGy, which were sensitive to irradiation and were the indicating bacteria of radiation dose setting. The lightness and yellowness of paprika powder decrease at high does, but total color difference and ASTA value had no significant difference, and the same to pungency by Scoville Test. So irradiation sterilization was perfect way to improve the quality paprika powder. (authors)

  4. Characteristic evaluation of papain irradiated with {sup 60}Co {gamma}-rays for the purpose of sterilization

    Energy Technology Data Exchange (ETDEWEB)

    Furuta, Masakazu; Ohashi, Isao; Oka, Masahito; Hayashi, Toshio [Osaka Prefectural Univ., Sakai (Japan). Research Inst. for Advanced Science and Technology

    1998-09-01

    To establish irradiation sterilization method for hybrid biomedical materials containing bioactive molecules together with artificial polymers, we employed dry powder and aqueous solution of papain as a model and examined radiation tolerance with {sup 60}Co {gamma}-rays. The dry powder and frozen aqueous solution showed significant resistance after 30-kGy irradiation, indicating that commercial irradiation sterilization method for disposable medical supplies was applicable. Unfrozen aqueous solutions (10mg/ml), in contrast, showed significant drop of enzymatic activity within the early period of irradiation (ca. 0.5kGy) but 40% of the activity was recovered at ca. 3-kGy before total inactivation at 15kGy. Taking various conditions including dose rate, concentration of enzyme, oxygen and nitrogen bubbling into irradiation inactivation experiments, we demonstrated that inactivation of papain could be controlled under anoxic condition, such as nitrogen bubbling, increasing sample volume at high dose rates and high concentration of enzyme where dissolved oxygen was consumed rapidly. It is suggested that radiation inactivation of papain in the aqueous solution was occurred through reversible oxidation of the sulfhydryl group at the active site by free radicals derived from radiolysis of water and dissolved oxygen. (author)

  5. Strand breaks and lethal damage in plasmid DNA subjected to 60CO-γirradiation

    International Nuclear Information System (INIS)

    Experiments with calf thymus DNA subjected to extracellular irradiation yield information on the role of direct and indirect effects in single-strand breakage, if this is evaluated with reference to the scavenger activity in respect of OH radicals. The role of the two processes in the occurrence of double-stand breaks and further damage leading to cell decay has so far remained largely obscure. It was the aim of the study described here to contribute to research in this field by performing in vitro experiments on biologically active DNA. For this purpose, DNA from pBR322 plasmids was irradiated in the presence of OH-radical scavengers. The number of single-strand and double-strand breaks was determined on the basis of the system's ability to eliminate OH radicals. In order to asses the influence of irradiation processes on the biological activity of DNA, investigations were carried out in E. coli for transformations caused by irradiated plasmid DNA. The results were interpreted in the light of theories about inhomogenous reaction kinetics put forward by Mark et al. (1989). It was finally discussed, which of the gamma-irradiation injuries occurring in DNA was to be held responsible for the inactivation of plasmid DNA and which enzymatic processes were additionally at work here. (orig./MG)

  6. Induction of mutation in Jujube (Zizyphus jujuba Mill) using tissue culture combined with 60Coγ-ray irradiation

    International Nuclear Information System (INIS)

    In vivo and in vitro mutagenesis techniques were assayed to explore effects of irradiation in jujube (Ziziphus jujuba Mill) improvement. 60Co γ-ray irradiated seeds and shoot tips of a land race of jujube originating in Shangdong province of China were micropropagated up to M1V4 generation on MS basal medium containing 2 mg/L BA and 0.4 mg/L IBA. The rooting MS medium contained 1 mg/L BA and 0.6 mg/L IAA, ZEA 1 mg/L, 2, 4-D 0.5 mg/L, and NAA 0.5 mg/L in different combinations. Adventitious buds were also produced from irradiated calli derived from leaf and hypocotyl fragments and the elongated adventitious buds rooted in vitro prior to green house transfer. Different doses (20 to 900Gy) were tested for in vitro explants as well as the jujube kernels irradiation. Six types of leaf shape and seven types of fruit shape mutations were observed and different ripening characters and growth habits were recorded in the orchard on putatively mutated mature trees. Even though there is a need for confirmation and molecular characterization, these mutations may be considered as a new and powerful way for jujube improvement in order to develop genotypes with promising value added traits. (author)

  7. {sup 60}Co irradiation effect on color in minimally processed cauliflower (Brassica spp)

    Energy Technology Data Exchange (ETDEWEB)

    Nunes, Thaise C.F.; Rogovschi, Vladimir D.; Santillo, Amanda G.; Villavicencio, Anna L.C.H. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)], e-mail: thaisecfnunes@hotmail.com; Michels, Robert [Hydrofarm Comercio e Representacao de Produtos Agricolas Ltda., Sao Roque, SP (Brazil); Pitombo, Ronaldo N.M. [Universidade de Sao Paulo (USP), SP (Brazil). Faculdade de Ciencias Farmaceuticas. Dept. de Alimentos e Nutricao Experimental

    2009-07-01

    Irradiation is a well-established process with clearly documented safety and efficacy. Consumers require a quality product that is convenient and has fresh-like characteristics and take product appearance, like color, into consideration as a primary criterion; it is considered a key role in food choice, food preference and acceptability. The objective of the present work was to investigate color degradation of cauliflower treated with gamma radiation during 14 days. Cauliflower irradiated samples with 0 and 1.5 kGy showed significant differences for value 'L' on the 10th and 14th day, respectively for value 'b' only the 0 kGy sample showed significant difference on day 10 of storage. On day 10 of shelf-life the irradiated cauliflower with doses of 1 and 1.5 kGy became more yellow compared to the control. The authors concluded that the treatment with low gamma radiation doses keeps the quality of fresh-cut vegetables. (author)

  8. Response of S. boulardii cells to {sup 60} Co irradiation and heat shock

    Energy Technology Data Exchange (ETDEWEB)

    Neves, M.J.; Andrade, A.S.R.; Santos, R.G. [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Belo Horizonte, MG (Brazil); Nicoli, J.R. [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Dept. Microbiologia

    1997-12-31

    Full text. Preparation of Saccharomyces boulardii, a non pathogenic yeast, has been widely used in Europe and other countries to prevent gastrointestinal disorders. However the mechanism of action of theses cells on the illness is unknown but the efficacy of S. boulardii depends on its viability. As trehalose is a well known viability protectant in yeast cells against several adverse conditions, we determined its level. We measured the level of trehalose in cells submitted to heat shock, gamma irradiation and simulation of gastric environmental, all these conditions are commonly found during the bio therapeutic production and in the patients oral treatment. Trehalose levels were higher in yeast cells surviving to gamma irradiation ({sup 60} Cobalt) than in control cells. S. boulardii cells growth in log phase and submitted to the heat shock (40 deg C). Accumulated more trehalose than S. cerevisiae and unlikely to these cells, the pool of trehalose accumulated in S. boulardii was mobilized very slowly (70% of the trehalose pool was present 5 hours after the return to the normal temperature 30 deg C). Our results suggested a rather different trehalose metabolism in S. boulardii when compared with S. cerevisiae and showed that one of the response to the stress of irradiation was an increasing on the level of intracellular trehalose

  9. Double strand breaks in DNA in vivo and in vitro after 60Co-γ-irradiation

    International Nuclear Information System (INIS)

    The questions of what the correlation is between double strand breaks in DNA in the cell and lethal radiation damage and by means of which possible mechanisms DNA double strand breaks could occur were studied. E. coli served as test system. In addition to this the molecular weight of the DNA from irradiated E. coli as a function of the radiation dose under various conditions was measured. This data was compared on the one hand to the survival of the cell and on the other hand to the formation of DNA double strand breaks in an aqueous buffer system, which in its ionic characteristics was similar to cell fluids. (orig./MG)

  10. The Effect of 60Co Gamma Irradiation on Various Fractions of Human Blood-Plasma Proteins

    International Nuclear Information System (INIS)

    The potential usefulness of employing ionizing radiation to cold-sterilize biomedical products has stimulated interest in characterizing the radiation sensitivity of various biologicals like vitamins, antibiotics, enzymes, antibodies and blood plasma fractions. This report presents findings on the sensitivity of haemagglutinin activity in human sera exposed to cobalt-60 gamma radiation. At doses tested between 0.2 and 5.0 Mrad, α-isohaemagglutinins were found to be more readily inactivated than ß-isohaemagglutinins, but neither was completely inactivated in this range. Sterility, clotting and antihaemophilic activity were also assessed on irradiated and freeze-dried preparations of human plasma with no significant differences in response detected. (author)

  11. High dose rate /sup 60/Co remote afterloading irradiation in cancer of the cervix in Haiti, 1977-1984

    Energy Technology Data Exchange (ETDEWEB)

    Streeter, O.E. Jr.; Goldson, A.L.; Chevallier, C.; Nibhanupudy, J.R.

    1988-06-01

    From 1977 through 1984, 293 previously untreated patients with biopsy proven carcinoma of the uterine cervix were treated by whole pelvis irradiation and high intensity 60Co remote afterloading (RAL) intrauterine tandem techniques in Haiti. The treatment results were analyzed retrospectively to evaluate the therapeutic results and prognostic factors of a strict protocol involving 40 Gy to the whole pelvis (2 Gy/day, 5 days/week). In addition, on the 5th day of the 3rd week, the first outpatient 60Co remote afterloading intracavitary insertion, delivering 7.5 Gy to point A with each insertion, repeated 3 times by a week separation for a total of 4 times. The total TDF for external beam plus RAL was 158 and 175 for early and late effects respectively. One hundred-four patients were evaluable after 1 year or more follow-up, with a median of 26.5 months. No evidence of disease (NED) by Stage at 1 year was: Stage I of 100% (3/3), Stage II of 82% (9/11), Stage III of 80% (47/59), and Stage IV of 58% (18/31). The post-therapeutic complication rate was 7.7%, with no fistulas or requirement of surgical intervention. Those with documented follow-up of at least 2 years (74 patients) had comparable survival to other high dose rate and low dose rate studies. This study shows that outpatient brachytherapy can be carried out without sophisticated and expensive equipment with minimal staff trained in radiation therapy. A detailed description of this outpatient RAL technique and results are described so that this method can be adapted to other developing and industrialized nations where cost containment is becoming a key issue.

  12. Influence of natural antioxidants on lipid composition of beef burgers submitted to irradiation in 60 Co source and electron beams

    International Nuclear Information System (INIS)

    Radiation processing has been employed in some countries as a mean of treatment to assure microbiological safety of meat and meat products, avoiding the occurrence of food-borne disease. The ionizing radiation may cause some undesirable changes on chemistry composition of food and the lipid oxidation is one of the main reactions. In meat products processing industry, the lipid composition is directly related to nutritional and sensory quality of the product. For preventing oxidation, use of antioxidants which can be synthetic or natural, has been practically applied in some products. Currently, most attention has been given to natural antioxidants from herbs and spices like rosemary and oregano. The aim this study was to assess the antioxidant effects of either rosemary and oregano extract in beef burgers submitted to irradiation in 60Co source with dose 6, 7 e 8 kGy, electron beams with dose 3,5 e 7 kGy and storage under freeze along 0, 45 e 90 days. The results showed that rosemary extract has the major antioxidant effects when it is used on heterogeneous food matrix like beef burger, but oregano extract was better efficient to delay lipid oxidation along storage time when it is used in synergism with rosemary and/or BHT/BHA. Although to have occurred changes in the fatty acids composition it was not possible to demonstrate a straight dependence of irradiation dose and/or storage time. Sensory analysis showed that between the samples prepared with natural antioxidants, the beef burger prepared with oregano has received better scores by panelists. Irradiated beef burger prepared with rosemary has received better scores when compared to non-irradiated one. The use of spices with antioxidant activity to avoid the oxidative damage in foods that contain fats in their formulation is thought to be promising to application in food facilities. (author)

  13. Effect of low dose irradiation of 60Co γ-rays on seed germination, seedling growth and enzymes activity of Lactuca sativa

    International Nuclear Information System (INIS)

    The seeds of Lactuca sativa were irradiated by different doses (10, 20, 30, 40, 50 Gy) of 60Co γ-rays. The effects of low dose irradiation on seed germination, seedling growth and enzymes activity were investigated. The results indicated that low dose irradiation could promote germination rate, germinating viability, germination rate in the field, root length and height of seedling. The suitable dosage for low dose irradiation for Lactuca sativa was 30 Gy. POD activity after irradiation treatment in the range of 10 to 50 Gy and CAT activity after irradiation treatment in the range of 20 Gy to 40 Gy was lower than that of control. (authors)

  14. 60Coγ射线损伤后小鼠造血系统生理指标的动态变化%Changes of Physiological Indexes in Hematopoietic System of Mice after 60Coγ Ray Irradiation

    Institute of Scientific and Technical Information of China (English)

    侯新然; 王晓波; 袭荣刚; 李忠亮; 高慧媛; 吴立军; 马晓梅

    2012-01-01

    Objective To explore the physiological variation of the hematopoietic system in mice after C07 ray irradiation. Methods Body weight, peripheral blood cell counts,the immune organ index and pathological changes in femoral bone marrow of mice were measured at different times after whole-body irradiation with C07 ray. The doses were 2,4,6 Gy respectively. Results The changes of peripheral blood of mice were different. The white blood cell counts declined most rapidly and obviously,followed by the platelet count,with a dose-effect relationship. Three days after radiation, the immune organ index of mice decreased significantly compared with the immune organ index in mice unirradiated. Myeloid elements in bone marrow were reduced and bone marrow hematopoiesis was depressed. Body weight of irradiated mice grew slowly, without significant difference with mice unirradiated. Conclusion 60Coγ ray radiation can decrease peripheral blood cells, decrease the immune organ index and inhibit bone marrow cell proliferation in mice.%目的 探索小鼠60Coγ射线损伤后造血系统各项生理指标的变化规律.方法 采用60Coγ射线对小鼠进行一次性全身照射,剂量分别为2、4、6 Gy,在不同时间分别测定小鼠体质量,外周血细胞计数,免疫器官指数及股骨骨髓的病理变化.结果 照射后小鼠的外周血象有变化,其中白细胞下降最迅速且明显,其次是血小板,且具有剂量效应关系.照射后3d免疫器官指数下降明显,与对照组比较具有显著性差异;骨髓造血细胞数目迅速减少,出现造血抑制;照射后小鼠体质量增加缓慢,但与对照组比较无显著性差异.结论 60Coγ射线照射可引起小鼠外周血细胞下降,免疫器官指数下降,并抑制骨髓造血细胞增生.

  15. Some Results Of Gamma-60Co Irradiation Application To Breed New Soybean Varieties At Institute Of Agricultural Genetics, 2001 - 2007

    International Nuclear Information System (INIS)

    To overcome the disadvantage characters and continue create new genetical materials in new Soybean variety selection to get high yield, resistance to with disadvantage conditions, good quality. The Institute of Agricultural Genetics successfully combinated the good particularities of mutant varieties DT84/DT83 and received DT2001 variety, and from mutant DT90/DT84 received new variety DT96. These varieties quite resists with rare good drought-tolerant, high yield from 2000 to 3500 kg/ha, appropriate with all of 3 crops: spring, summer and winter and have wide adaptation. DT96, DT2001 varieties were adopted by MARD Scientific Council as New National Varieties. cooperated with Dalat Institute of Nuclear research treated the Gamma - 60Co Irradiation on Soybean and Vegetable Soybean. Irradiated dry seed of 3 varieties DT96, DT2001, DT158 with dose levels of 150, 200, 250 Gy in order to shorten the rise time and strengthen the drought resistance. At M3 generation had selected 3 lines D.96/26, D.01/58, D.58/08, D.58/224, which have rise time from 3 to 5 day shorter and yield higher than control (D.96/150, D.01/245). Irradiated the 2 vegetable Soybean DT02 (to ameliorate its thick shell, improve sweetness and resist with collapse) and DT06 (to ameliorate its heat resistance) with dose levels of 100, 140, 180, 220 Gy were received the generations and now continue being researching the M3 generation in 2007 summer crop. (author)

  16. Obtained and evaluation of antisera raised against irradiated crotalic whole venom or crotoxin in 60 Co source

    International Nuclear Information System (INIS)

    Snake bite is a great Public Health problem in our country. The accidents with snakes from Crotalus genus are the most severe. About 1% of the victims die without seratherapy. The antivenons are obtained from hyper immune horse plasma. During the production these animals present signs of envenoming that result in a decrease of organic resistance besides the horses maintenance is very expensive and the producers are fewer, so the sera production is restrict. Many techniques which could reduce the venoms toxicity and increase the sera production using chemical and physical agents have been studied. The gamma rays are excellent tool to detoxify venoms and toxins. It is able to modify protein structures that decrease lethally, toxic and enzymatic activities without modifying the immunogenicity. So, it is important evaluate the sera production in rabbits using gamma rays detoxified venom and crotoxin as immunogen and their power as reagents in immuno assays. In order to obtain the antisera, Crotalus durissus terrificus whole venom or isolated crotoxin was irradiated with 2.000 Gy in 60 Co source, in a 150 mM NaCl solution, and inoculated in rabbits. The sera production were screened by immunoprecipitation, immuno enzymatic (ELISA) and immunoradiometric (IRMA) assays. The specificity was studied by immuno-electrophoresis, ELISA and western blot techniques. The neutralizing power was evaluated by neutralization of phospholipase A2 activity of toxin in vitro. The antisera were used as reagents in antigen capture assays ELISA and IRMA immuno assays to detect circulant antigens in sera of mice experimentally inoculated with crotalic venom or crotoxin. The results showed that both detoxified venom or crotoxin were good immunogens, and they were able to induce antibodies that could recognize non-irradiated venom or isolated crotoxin. The data suggest that those antibodies present more specificity and higher in vitro neutralizing power, when compared with commercial

  17. Differential expression profiles of microRNAs in liver of 60Co γ-ray irradiated mice

    International Nuclear Information System (INIS)

    Objective: To investigate the differential expression profiles of microRNAs in the liver of 60Co γ-ray irradiated mice using microRNA microarray and to explore their main functions by bioinformatic analysis. Methods: After SPF C57BL/6J mice expose to 4 Gy-single whole body radiation,total number of peripheral WBC and the fMNPCE were measured at 3 d.The differentially expressed miRNAs in mouse liver were detected with miRNA microarray, miRNA-124 and miR-34a were confirmed by real time RT-PCR assay. Bioinformatic analysis was applied to explore target genes and the main functions of the differential expressed miRNAs. Results: Compared with control group, the total number of peripheral WBC decreased (t=2.87, P<0.05), while the fMNPCE in bone marrow increased (t=-2.91, P<0.05) after 4 Gy γ-ray irradiation.miRNA microarray revealed that 17 miRNAs were differentially expressed, in which 9 up-regulated, 8 down-regulated. The expression levels of miR-124 and miR-34a were coincident with the result of real time RT-PCR. GO analysis showed that some pathways including adherens junction and cell cycle were suppressed, while some immune-related pathways were activated. Conclusions: miR-34a and miR-194 were involved in the regulation of acute radiation damage, some other miRNAs including miR-124, miR-382 and miR-92a* also played important roles in radiation process. (authors)

  18. Evaluation by EPR of potential antioxidant components of {sup 60}Co-irradiated varieties of soybean; Avaliacao por RPE de componentes com potencial antioxidante de variedades de soja irradiadas com {sup 60}CO

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Marcos Ronaldo Ramos de

    2009-07-01

    Brazil is today the second main producer of soybean in the world with a planted ground of about 21 million hectares and an annual production of 60 million tons in 2008, being slight more than a fourth of the annual production. The presence of flavonoids, particularly isoflavones in soybean products has been related as important for human health. It has been suggested that flavonoids play a role in the protection of plants by screening vital cellular components from damaging UV radiation. Electron paramagnetic resonance (EPR) spectroscopy can measure free radicals produced by dissociation molecules resulting from irradiation. It has been successfully employed for the detection of some irradiated food products. Twenty one Brazilian soybean cultivars from two crops were gamma-irradiated with a {sup 60}Co source and evaluated by EPR. Correlation coefficients were made among the central EPR signal (g = 2.0039) and the total and partial isoflavones contents. There was no correlation with total contents, though glicitein and acetyl-daidzin showed a negative correlation. Even 7 months after irradiation the intensity of central EPR signal were high enough to distinguish the irradiated samples. EPR measurements of separated parts of the grain were more efficient for that purpose, particularly from hilum and coat. The radiation did not change substantially the total isoflavone contents, although there were some evidences suggesting some conversion of glycosides to aglycones. (author)

  19. Biochemical and pharmacological studies of native and irradiated crotamine with gamma radiation of {sup 60}Co; Estudo bioquimico e farmacologico das crotaminas nativa e irradiada com radiacao gama de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Mitake, Malvina Boni

    2000-07-01

    Ionizing radiation can change the molecular structure and affect the biological properties of biomolecules. This has been employed to attenuate animal toxins. Crotamine is a strongly basic polypeptide from the South American rattlesnake venom, composed of 42 amino acid residues. It induces skeletal muscle spasms leading to a spastic paralysis of hind limbs in mice. The objective of this thesis was carry out biochemical and pharmacological studies of native and irradiated crotamine with {sup 60} Co. Crotamine was purified from Crotalus durissus terrificus venom by Sephadex G-100 gel filtration followed by ion exchange chromatography, using a Fast Performance Liquid Chromatography (FPLC) system. It was irradiated at 2 mg/ml in 0.15 M Na Cl with 2.0 kGy gamma radiation emitted by a {sup 60} Co source. The native and irradiated crotamine were evaluated by biochemical characterization, toxic activity (LD{sub 50} and biodistribution. The native and irradiated crotamine were labelled with 29.6 MBq of {sup 125} I using chloramine T method, and separated in a Sephadex G-50 column. Male Swiss mice (35{+-} 5 g), were injected i.p. with o.1 mL (2.4 x 10{sup 6} cpm/mouse) of {sup 125} I native crotamine or with 0.4 mL (1.3 x 10{sup 6} cpm/mouse) of {sup 125} I irradiated crotamine. At 0.08; 0.25; 0.5; 1; 2; 4; 8; 12 and 24 hours the animal were killed by ether inhalation. Blood, spleen, liver, kidneys, brain, lungs, heart, and skeletal muscle were collected in order to determine radioactivity content. The results showed that gamma radiation did not change the protein concentration, the electroforetic profile or the primary structure of the protein, although differences were shown by spectroscopic techniques. The gamma radiation diminished the toxicity of crotamine, but it did not abolish bioactivity. Biodistribution studies showed that native and irradiated crotamine have hepatic metabolism and renal elimination. The native and irradiated crotamine have affinity by skeletal

  20. The effects of seeds irradiation with 60Co γ-ray on seed germination and shoot growth of Brassica campetris L. ssp. Chinensis var. utilis

    International Nuclear Information System (INIS)

    Seeds of Brassica campetris L. ssp. Chinensis var. utilis pre-soaked with water for 1h were irradiated with 60Co γ-ray. Irradiation delayed the germination of seeds but did not affect the germination rate. The height, root length, fresh weight and survival rate of seedlings decreased with the increasing of irradiation dose. There were significant negative correlation between death rate and the height, root length and fresh weight. The dose of 200 - 300 Gy were recommended as suitable irradiation doses for pre-soaked seeds

  1. 60Co-γ辐射对菊苣种子发芽及幼苗生理的影响%Effects of 60Co-γ-rays Irradiation on Chicory Seed Germination and Seedling Physiology

    Institute of Scientific and Technical Information of China (English)

    张玉; 白史且; 李达旭; 王曾珍

    2013-01-01

    Chicory seeds were irradiated with 60Co-γ-rays at 100, 150, 200 and 300 Gy to determine germination rate and physiological characteristics from mutagenic effects of irradiation doses. Results showed that seed germination rate decreased as the irradiation dosage increased. There was significant difference between 200 Gy irradiation dosage and control(P<0. 05). Superoxide dismutase (SOD) activities, peroxi-dase (POD) activities, soluble protein and soluble sugar contents first increased then declined with a gradual irradiation increase, and differed significantly between control and 150 Gy, 100 Gy, 150 Gy, 150 Gy treatments (P<0. 05), respectively. MDA content increased with increased irradiation dosage, and there was significant difference between control and the treatments above 150 Gy dose (P<0. 05). The protein content first increased then declined, and differed significantly between control and treatments (P<0. 05). It was concluded that the mutagenic effect of chicory irradiated by low dosage (below 150 Gy) was not obvious. However, physiological and biochemical characteristics of chicory irradiated above 200 Gy were significantly altered. The study provides a theoretical basis for chicory radiation mutation breeding.%为明确60 Co-γ射线对菊苣(Cichorium intybus L.)辐照诱变效果,采用不同剂量60Co-γ射线(100 Gy,150 Gy,200 Gy和300 Gy)辐照处理菊苣种子,测定其发芽率和相关生理指标.结果表明:随着辐照剂量的增加,菊苣发芽率降低,200 Gy以上辐照处理发芽率与对照差异显著(P<0.05);超氧化物歧化酶(SOD)、过氧化物酶(POD)活性、可溶性蛋白和可溶性糖含量随着辐照剂量增加均表现为先升后降的趋势,分别在辐射剂量为150 Gy,100 Gy,150Gy,150 Gy及以上时与对照差异显著(P<0.05);丙二醛(MDA)含量随着辐照剂量增加而逐渐升高,150 Gy以上辐照处理与对照差异显著(P<0.05);蛋白含量呈现先增后减的趋

  2. The effects of 60Co γ-ray irradiation on the DC characteristics of enhancement-mode AlGaN/GaN high-electron-mobility transistors

    Institute of Scientific and Technical Information of China (English)

    Chen Chao; Tian Ben-Lang; Liu Xing-Zhao; Dai Li-Ping; Deng Xin-Wu; Chen Yuan-Fu

    2012-01-01

    The effects of 60Co y-ray irradiation on the DC characteristics of AlGaN/GaN enhancement-mode high-electronmobility transistors (E-mode HEMTs) are investigated.The results show that having been irradiated by 60Coγ-rays at a dose of 3 Mrad (Si),the E-mode HEMT reduces its saturation drain current and maximal transconductance by 6% and 5%,respectively,and significantly increases both forward and reverse gate currents,while its threshold voltage is affected only slightly.The obvious performance degradation of E-mode AlGaN/GaN HEMTs is consistent with the creation of electronegative surface state charges in the source-gate spacer and gate-drain spacer after being irradiated.

  3. 255Gy irradiated tachyzoites of Toxoplasma gondii induce intestinal immune response in C57BL/6J immunized by oral route

    Energy Technology Data Exchange (ETDEWEB)

    Galisteo Junior, Andres Jimenez; Alves, Janaina Baptista [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Lab. de Biologia Molecular]. E-mail: galisteo@usp.br; Hiramoto, Roberto Mitsuyoshi [Instituto Adolfo Lutz, Sao Paulo, SP (Brazil). Secao de Parasitoses Sistemicas]. E-mail: hiramoto@usp.br; Carmo, Claudia Villano do; Andrade Junior, Heitor Franco de [Instituto de Medicina Tropical de Sao Paulo, Sao Paulo, SP (Brazil). Lab. de Protozoologia]. E-mail: hfandrad@usp.br

    2005-07-01

    Toxoplasmosis, a prevalent widespread infection in man and animals, occurs mainly through ingestion of water and food contaminated with oocyst from cat feces, causing usually benign disease in humans, except in intrauterine fetal infection or in immunodeficient patients. We study the oral route for the development of a vaccine for toxoplasmosis, using parasites irradiated with 60 Cobalt, as an alternative for vaccine development to this worldwide parasitic infection. We evaluated the development of immunity at serum or mucosal levels, and their efficiency in protect the mice against challenge with oral cysts of the ME-49 strain. C57Bl/6j isogenic mice were immunized by oral route with 10{sup 7} 255 Gy irradiated tachyzoites from RH strain, at several protocols using milk as anti-peptic adjuvant and alum hydroxide as antacid. The preparations of irradiated tachyzoites induced production of serum IgG and IgA in immunized mice, as determined by ELISA, with IgG2a as the dominant subclass, similar to chronic infection. Their use with adjuvant allowed the excretion of significant amounts of IgA in stools also IgG, despite a lesser extent. All oral preparations induced some quantitative protection against challenge, which was similar to the parenteral route only isolated alum hydroxide was used as adjuvant. All these data support the possibility of the development of an oral vaccine against toxoplasmosis, using irradiated tachyzoites, which would be possible tool in near future for use in field baits, for immunizing either domestic or wild felids. (author)

  4. The effect of combination of ErbB2 RNAi and 60Co γ-irradiation on U251 cell apoptosis

    International Nuclear Information System (INIS)

    To construct erythroblastic leukemia viral oncogene homolog 2 (ErbB2) small interference RNA (siRNA) expression vector and to study its effect on U251 cell line proliferation and apoptosis combining with 60Co γ-irradiation. ErbB2 specific 19bp oligonucleotides were designed and synthesized. These oligonucleotides were annealed to form the double strand DNA fragments, which was cloned into pSilence2.1-U6-H1 vector. The recombinant pSilence2.1-ErbB2 expression construct was confirmed by Hind III and BamH I double digestion and sequencing. The pSilence 2.1-ErbB2 was transfected into U251 cell. Cellular proliferation activities were assayed by tetrazolium bromide (MTT) colorimetry. The apoptosis of transfected U251 cell was examined with Hoechst 33258 staining and Annexin-V kit. Psilence 2.1-ErbB2 expression vector was successfully constructed and it can effectively inhibit pro- liferation(p60Co γ-irradiation, the effect of inhibiting proliferation was more significant compared with non-irradiated U251 cells(p60Co γ-irradiation can enhance the inhibitory efficiency in U251 cell line. (authors)

  5. Numerical simulation of 60Co-gamma irradiation effects on electrical characteristics of n-type FZ silicon X-ray detectors

    Science.gov (United States)

    Vigneshwara Raja, P.; Rao, C. V. S.; Narasimha Murty, N. V. L.

    2016-07-01

    This paper describes the gamma irradiation effects on the electrical characteristics of n-type float zone (FZ) silicon detectors by incorporating a 4-level 60Co-gamma radiation damage model in the commercial device simulator for plasma X-ray tomography diagnostics. In the simulations, a segmented n-type silicon detector (i.e. p+-n-n+ structure) is considered with varying substrate resistivity (ρ = 5.4, 2.5, and 0.3 kΩ cm). The simulation results have been validated with the reported experimental measurements carried out on similar device structures. The 60Co-gamma irradiation induced changes in the electrical characteristics of the detectors are analyzed up to the dose of 3500 Mrad. The possible gamma induced degradation in the X-ray response of the detectors is investigated from the changes in the effective doping concentration and the leakage current of the detectors. The survival of the gamma irradiated detectors is predicted from the simulation studies. The comparison between the 60Co-gamma and 14.1 MeV neutron irradiation effects (typical fusion environments) on silicon detectors is attempted.

  6. Transfection of p27 kip1 enhances radiosensitivity induced by 60Coγ-irradiation in hepatocellular carcinoma HepG2 cell line

    Institute of Scientific and Technical Information of China (English)

    Xiao-Xiang Guan; Long-Bang Chen; Gui-Xia Ding; Wei De; Ai-Hua Zhang

    2004-01-01

    AIM: To study the cell cycle alterations of human hepatoma cell line HepG2 in vitro after 60Co γ-irradiation and further to examine the mechanisms underlying the enhancement of radiosensitivity to γ-irradiation in HepG2 transiently transfected with wild type p27kip1.METHODS: The proliferation of HepG2 cells was evaluated with MTT assay, and the cell cycle profile and apoptosis were assessed by cell morphology, DNA fragmentation analysis and flow cytometry. HepG2 cells were transfected with p27kip1 wild type by using Lipofectamine (LF2000), and the expression and subcellular localization of p27kip1 in HepG2were detected by immunocytochemistry.RESULTS: 60Co γ-irradiation inhibited the growth of HepG2cells in a dose-dependent manner. Apoptosis of HepG2 cells was induced 48 h after γ ray exposure. Furthermore research was carried out to induce exogenous expression of p27kip1in HepG2. The expression of p27kip1 induced G0/G1 phase arrest in HepG2 cells. The overexpression of p27kip1 enhanced 60Co γ-irradiation-induced radiosensitivity in HepG2 cells.CONCLUSION: Overexpression of p27kip1 is a rational approach to improve conventional radiotherapy outcomes, which may be a possible strategy for human hepatoma therapy.

  7. Cytotoxicity evaluation of Diethyltoluamide (DEET) in Perna perna (Linnaeus, 1758) mussels non-irradiated and irradiated with 60Co gamma radiation

    International Nuclear Information System (INIS)

    Recent studies have identified the presence of several emerging pollutants in aquatic environments. The occurrence in different environmental matrices has been continuously reported, highlighting the need for toxicity studies. The DEET (N,N-diethyl-meta-toluamide) is the active ingredient used in most insect repellents, and is present in many commercially available formulations. Apart from chemical pollutants, aquatic organisms are subject to exposure of ionizing radiation from natural sources or in the vicinity of nuclear power plants. The present study evaluated the toxicity of DEET in organisms irradiated and non-irradiated with 60Co gamma radiation, and the effects that radiation causes in lysosomes of hemocytes of Perna perna mussel. For this purpose, assays were performed to identify the acute toxicity of DEET concentration and the dose of gamma radiation able to cause mortality. Subsequently, cytotoxicity assays were carried out to assess the stability of the lysosomal membrane in organisms exposed to ionizing radiation and DEET. According to the results obtained in acute toxicity tests, the concentration of DEET that causes mortality of 50% exposed organisms (LC50) is 114,27 mg L-1, and the radiation dose that causes mortality (LD50) is 1068 Gy. In the cytotoxicity assays, the concentration of the non-observed effect (NOEC) for irradiated and non-irradiated organisms 0.0001 mg L-1 and observed effect concentration (LOEC) at concentrations above this. The IC25 (72h) for non-irradiated organisms was 0.0003 mg L-1 and IC50 (72h) was 0.0008 mg L-1 for irradiated and non-irradiated organisms. Despite of the concentrations of effect found in this study were higher than in the environment, both measurements are in the same order of magnitude and should be also take into account the possible synergistic effects of DEET with other contaminants in the aquatic environment. (author)

  8. Analysis of the frequency of unstable chromosome aberrations in human lymphocytes irradiated with {sup 60}Co; Analise da frequencia de alteracoes cromossomicas instaveis em linfocitos humanos irradiados com {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Mendonca, Julyanne C.G.; Mendes, Mariana E.; Lima, Fabiana F., E-mail: july_cgm@hotmail.com, E-mail: mendes_sb@hotmail.com [Centro Regional de Ciencias Nucleares (CRCN-NE/CNEN-PE), Recife, PE (Brazil); Santos, Neide, E-mail: santos_neide@yahoo.com.br [Universidade Federal de Pernambuco (CCB/UFPE), Recife, PE (Brazil). Departamento de Genetica

    2013-07-01

    The aim of this study was to analyze the frequency of unstable chromosomal aberrations induced by gamma radiation from a {sup 60}Co source at two different doses. Samples were obtained from a healthy donor and exposed to {sup 60}Co source (Gammacel 220 ) located in the Department of Nuclear Energy of Pernambuco Federal University (DEN/UFPe/Brazil) with a rate of air Kerma to 3,277 Gy/h. Exposures resulted in absorbed dose 0.51 Gy and 0.77 Gy. Mitotic metaphases were obtained by culturing lymphocytes for chromosome analysis and the slides were stained with 5% Giemsa. Among the unstable chromosomal aberrations the dicentric chromosomes, ring chromosomes and acentric fragments were analyzed. To calculate the significance level the chi - square test was used, considering relevant differences between the frequencies when the value of p < 0.05. To calculate the significance level of the chi - square test was used, considering relevant differences between the frequencies when the value of p < 0.05. The results showed that there was significant difference of the frequencies of dicentric chromosomes (from 0.18 to 0.51 to 0.37 Gy to 0.77 Gy), however there was no statistically significant difference between the frequencies of acentric fragments ( 0.054 to 0, 51 Gy to 0.063 to 0.77 Gy) and ring chromosomes (0.001 to 0.51 Gy to 0.003 to 0.77 Gy). The low number of rings is found justified, considering that in irradiated human lymphocytes, its appearance is rare relative to dicentrics. The results confirm that dicentrics are the most reliable biomarkers in estimating dose after exposure to gamma radiation. These two points will make the calibration curve dose-response being built for Biological Dosimetry Laboratory of CRCN-NE/CNEN.

  9. The response to climate factors for the different specificity in M2 physiology of 60Co γ-ray irradiated flowers

    International Nuclear Information System (INIS)

    Physiology and climate factor of different M2 flower (Begonia tuberhybrida Voss and Dahlia pinnata Cav root) after 60Co γ-ray irradiated were determined. The result showed that transpiration rate, stoma conductance, intercellular CO2 concentration of M2 of Dahlia pinnata Cav are affected obviously with climate factors, however, effect on the photosynthetic speed was not found, and M2 of Begonia tuberhybrida Voss was not significant for physiology to climate

  10. Differential gene expression in human fibroblasts after alpha-particle emitter (211)At compared with (60)Co irradiation

    DEFF Research Database (Denmark)

    Danielsson, Anna; Claesson, Kristina; Parris, Toshima Z;

    2013-01-01

    trastuzumab monoclonal antibody (0.25, 0.5, and 1 Gy) and (60)Co (1, 2, and 3 Gy). Results: We report gene expression profiles that distinguish the effect different radiation qualities and absorbed doses have on cellular functions in human fibroblasts. In addition, we identified commonly expressed transcripts...

  11. The effects of chloramphenicol and 60Co irradiation on the experimental mycosis in mice inoculated intragastrically with two kinds of pathogenic yeast-like fungi

    International Nuclear Information System (INIS)

    Two kinds of pathogenic yeast, Cryptococcus neoformans and Candida albicans were inoculated into the stomachs of mice. The effects of intragastric administration of chloramphenicol (CM) and systemic irradiation with 60Co on the acceralation of infection were studied in intragastrically inoculated mice. When Cryptococcus neoformans were simply inoculated, it was difficult for them to reside in the alimentary canal to cause infection. In case of candida albicans inoculated simply, they were also unable to induce infection though they remained in the alimentary canal for a long time. In the experiment combining intragastric administration of the fungi and CM some mice contracted the disease and died. The effect of CM in causing infection was not proved. In the experiments combining 60Co irradiation and intragastric administration of CM and fungi, a high incidence of fungus infection was noted with both Cryptococcus neoformans and Candida albicans. Fungus lesions were observed in many organs, and in all of the mice which died systemic fungus lesions were noted. It was shown that systemic irradiation with 60Co had a specific effect on facilitating the infection. In these studies no difference was observed in the incidence of the fungus disease in respect of the origin of the causative fungi. (J.P.N.)

  12. Effect of 60Co γ irradiation with seed and shoot-tip of Brassica campestris L. var on its culture in vitro

    International Nuclear Information System (INIS)

    The survival rate in vitro of shoot-tips of Brassica campestris L. var from seeds irradiated by 60Co γ-rays decreased with the increase of dose. Irradiation inhibited proliferation of shoot-tip, induction of callus from cotyledons and differentiation of the callus. The age of explant contributed to the effect of irradiation in the culture. Irradiation stimulated the proliferation of shoot-tip with dose less than 50 Gy. Based on the effect of irradiation in the tissue culture, the effective dose recommended was about 200 Gy for seeds, 50-100 Gy for pre-soaked germinating seeds and 40-70 Gy for shoot-tips in vitro, respectively

  13. Characterization of 60Co and 137Cs beams from irradiator Shepherd 81 - 14D of LNMRI/IRD in radiation protection

    International Nuclear Information System (INIS)

    The objective of this work is the characterization of 60Co beams and the 137Cs irradiator Shepherd 81-14D acquired by the National Metrology Laboratory of Ionising Radiation of the Institute of Radiation Protection and Dosimetry (LNMRI /IRD), for use in calibration of measuring instruments of gamma radiation in air kerma and ambient dose equivalent, irradiation of films and TLD's. Field homogeneity measurements were carried out, calculated input and output time of the source and commissioning of sources. (author)

  14. Effect of hydrogen on change carrier dissipation in 60Co irradiated by γ-quanta and non-alloyed n-type GaAs

    International Nuclear Information System (INIS)

    The pretreatment in hydrogen plasma (the hydrogenation) influences on the charge carrier dissipation processes in the non-alloyed gallium arsenide of n-type with no = (5...7) centre dot 1015 cm-3 and μo = (5...6) centre dot 10 13 cm2 / (V centre dot c) irradiated by γ-quantum 60Co was studied. The comparison of experimental dependence μ (T) with the designed one in the temperature range 77...291 K for non-hydrogenized and hydrogenized non irradiated and γ-quantum irradiated crystals was carried out. It is shown that the main dissipative mechanism that determine the charged carrier mobility in the non hydrogenized material is the dissipation on the charged centers - the radiation defects in the γ-quantum irradiated GaAs; the presence of double ionized defects is possible

  15. Effects of 60Co γ-ray irradiation on microstructure and ferroelectric properties of Bi3.25La0.75Ti3O12 thin films

    Science.gov (United States)

    Wang, Zan; Jiang, Wei; Li, San-xi; Tong, Jun-sheng

    2016-01-01

    Bi3.25La0.75Ti3O12 (BLT) thin films were deposited on Pt/Ti/SiO2/Si substrates through sol-gel method. Films underwent 60Co γ-ray irradiation with different doses; 0, 50, 100 and 150 kGy, respectively. Impacts of γ-ray on the microstructure, ferroelectric properties, leakage current density and fatigue characteristic were studied in detail. The results of SEM images show that grain patterns become irregular. Remnant polarization (2Pr) and coercive field (2Ec) decrease with irradiation dose increase. C-V curves reveal obvious asymmetry along y-axis. The irradiated thin films display lower leakage current density and fatigue endurance up to more than 1010 switching cycles. These results suggest that radiation can improve the film performance in some areas.

  16. Dose-rate effect on chromosomal aberrations induced by 60Co γ-rays irradiation in human peripheral blood lymphocyte

    International Nuclear Information System (INIS)

    To estimate exactly the biological dose of persons exposed to different dose rate, human peripheral blood was exposed to 60Co γ-rays in vitro at low, middle and high dose rates respectively and chromosome samples were prepared, then dose-response curves were established according to the dicentrics and ring frequencies. The result showed that the aberration frequency at same dose level increased with dose rate and there was an obvious dose-rate effect. Absorbed dose estimated with low dose-rate dose-response curve was higher markedly than that with high dose-rate dose-response curve. So, considering the effect of dose-rate, approximate dose-rate dose-response curve should be chosen when absorbed dose estimation and the result will be credible. (authors)

  17. Differential Expression of Retrotransposon WIS 2-1A Response to Vacuum, Low-Energy N+ Implantation and 60Coγ-ray Irradiation in Wheat

    Science.gov (United States)

    Zhao, Huiru; Gu, Yunhong; Ya, Huiyuan; Jiao, Zhen; Qin, Guangyong

    2009-02-01

    Mutagenesis and retrotransposons have a close relationship, but little attention has been paid yet to the activity of retrotransposons produced by physical mutagens. The variation of retrotransposon WIS 2-1A activity in wheat (Triticum aestivum L.) embryos at three different growth times (30 h, 45 h and 60 h) was investigated after they had been treated with N+ implantation in a vacuum of 5 × 10-2 Pa and irradiation by 60Coγ-ray respectively. For each of the three growth times the expression of WIS 2-1A showed almost entirely a same trend of downregulation, upregulation, then downregulation, and upregulation again with the increase in dose of N+ implantation, but the expression appeared irregular with the increase in irradiation of 60Coγ-ray. In conclusion, the acutely activating effect of WIS 2-1A stimulated by vacuum and high dose N+ implantation within a shorter incubation time may provide a convenient tool to advance the research on mutagenic breeding and function genes.

  18. Differential Expression of Retrotransposon WIS 2-1A Response to Vacuum,Low-Energy N+ Implantation and 60Coγ-ray Irradiation in Wheat

    Institute of Scientific and Technical Information of China (English)

    ZHAO Huiru; GU Yunhong; YA Huiyuan; JIAO Zhen; QIN Guangyong

    2009-01-01

    Mutagenesis and retrotransposons have a close relationship,but little attention has been paid yet to the activity of rtrotransposons produced by physical mutagens. The variation of retrotransposon WIS 2-1A activity in wheat(Triticum aestivum L.)embryos at three different growth times(30 h,45 h and 60 h)was investigated after they had been treated with N+ implantation in a vacuum of 5x10-2 Pa and irradiation by 60Coγ-ray respectively.For each of the three growth times the expression of WIS 2-1A showed almost entirely a same trend of downregulation,upregulation,then downregulation,and upregulation again with the increase in dose of N+ implantation,but the expression appeared irregular with the increase in irradiation of 60Coγ-ray.In conclusion,the acutely activating effect of WIS 2-1A stimulated by vacuum and high dose N+ implantation within a shorter incubation time may provide a convenient tool to advance the research on mutagenic breeding and function genes.

  19. Production of ochratoxin a by aspergillus ochraceus NRRL-3174 before and after exposures to /sup 60/Co irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Applegate, K.L.; Chipley, J.R.

    1976-03-01

    Spores from the toxigenic organism Aspergillus ochraceus NRRL-3174 were exposed to specific levels of gamma irradiation and then allowed to germinate on selected media. Increases in ochratoxin A production by irradiated, compared to non-irradiated, spores were observed after inoculation of spores onto a cracked red wheat or into a synthetic liquid medium. Variations in daily ochratoxin production were also observed for control and irradiated spore-derived cultures developing on both media, with maximum toxin production varying from 7 to 11 days of incubation. The most notable increases in ochratoxin A production occurred from cultures developing from spores having been irradiated with 10, 25, or 50 krad. Exposures to 400 or 600 krad resulted in complete inhibition of spore germination and, consequently, no ochratoxin production. Of the two substrates used, wheat and synthetic, the quantities of ochratoxin A produced were significantly lower in the synthetic media than on the natural substrate. Higher and more rapid toxin production occurred from spores having been irradiated with 10, 25, 50, and 100 krad than occurred from the non-irradiated control spores when grown on synthetic media. Cultures derived from spores having been exposed to 10, 25, and 50 krad produced significantly higher levels of ochratoxin A after 8 days of incubation on natural substrate than did the controls. Analysis of variance revealed that substrate, length of incubation, as well as irradiation levels all affected the time required to produce maximum levels of ochratoxin A.

  20. Immune response against the irradiated Bothropstoxin-1 with 60Co: identification of main cytokines involved and the participation of scavengers substances

    International Nuclear Information System (INIS)

    Considering the effects of gamma radiation on proteins and the ability of immune system to recognize modified macromolecules, we have identified the major cytokines involved in immune response of B10.PL, BALB/c and Knockout- IFNγ mice exposed to native or irradiated bothropstoxin-1 (BTHX-1), in the presence and absence of scavengers substances. In order to evaluate possible molecule structural modifications after being irradiated (60Co gamma rays), bothropstoxin-1 was submitted to SDS-PAGE analyses. Our results indicated that irradiation process has promoted modifications in the BTHX-1 molecule, however, in the presence of scavengers and even after irradiation process, the main band of toxin was preserved (14 kDa). Sera of animals immunized with the native or irradiated toxin, in the presence or not of scavengers, were analyzed in order to quantify specific isotopes. While the native BTHX-1 induced a predominant Th2 response, the irradiated toxin apparently promoted a switch towards a Th1 pattern. The toxin, when irradiated in the presence of t-butanol, induced to a lower production of IgG2b (Th1 response) if compared with the irradiated toxin without scavengers. We also performed a Real-time PCR to quantify the expression of cytokines IFN-γ, IL-2, IL-4 and IL-10 in spleen cells from mice. The cells of B10.PL and BALB/c mice immunized with native BTHX-1 and in vitro stimulated with irradiated toxin, showed higher expression of IFN-γ and IL-2 (Th1 response) than the control sample. The cells of Knockout-IFNγ mice immunized with native BTHX-1 showed higher expression of IL-4 (Th2 response). The cells obtained of B10.PL and BALB/c mice immunized with BTHX-1 + t-butanol, showed higher expression of IL-4 and IL-10, respectively. These facts reinforce the involvement of OH in the modulation of immune response against the irradiated toxin. (author)

  1. Rapid rejoining of DNA strand breaks in resting human lymphocytes after irradiation by low doses of 60Co γ rays or 14.6-MeV neutrons

    International Nuclear Information System (INIS)

    The production and repair of DNA strand breaks was studied in human lymphocytes by means of a sensitive fluorometric technique. Lymphocytes were isolated by conventional methods and air-equilibrated suspensions were irradiated by low doses (less than or equal to 2 Gy) of either 60Co γ rays or 14.6-MeV neutrons at 00C. The apparent yield of initial strand breaks induced by neutrons was only 36% of that induced by γ rays, in agreement with the observations of other workers. Resting lymphocytes were found to be proficient in their ability to rejoin γ-induced strand breaks at 370C; rejoining followed biphasic kinetics, with 70% of the breaks disappearing with a half-life of about 3 min. Although the initial number of breaks induced by neutron irradiation was low, after 20 min of incubation the residual number of breaks was very similar for the two forms of radiation

  2. 60Coγ射线辐照不同麻种种子的细胞学效应%Cytological Effects on Seeds of Hibiscus cannabinus and Cannabis sativa Irradiated by 60Coγ ray

    Institute of Scientific and Technical Information of China (English)

    周立人; 吴雪琴; 魏晓飞; 李布青

    2003-01-01

    通过5种不同剂量的60Coγ射线对两个不同麻种3个品种材料的种子辐照效应研究表明,低剂量的60Coγ射线辐照能促进麻类作物根尖细胞的有丝分裂.但随着γ射线辐照剂量的增加,根尖细胞有丝分裂指数呈不同程度的下降趋势.60Coγ射线辐照能诱发根尖细胞的染色体畸变和核畸变,出现桥、断片、落后染色体、微核、双核、小核等多种畸变类型,并且辐照剂量越大,根尖细胞畸变类型越多,变异频率越高.研究结果还表明不同麻种、同一麻种不同品种对60Coγ射线的辐射敏感性不同,红麻中杂红305最强,红麻福红2号次之,大麻最弱.

  3. Structural and optical analysis of 60Co gamma-irradiated thin films of polycrystalline Ga10Se85Sn5

    Science.gov (United States)

    Ahmad, Shabir; Asokan, K.; Shahid Khan, Mohd.; Zulfequar, M.

    2015-12-01

    The present study focuses on the effects of gamma irradiation on structural and optical properties of polycrystalline Ga10Se85Sn5 thin films with a thickness of ∼300 nm deposited by the thermal evaporation technique on cleaned glass substrates. X-ray diffraction patterns of the investigated thin films show that crystallite growth occurs in the orthorhombic phase structure. The surface study carried out by using the scanning electron microscope (SEM) confirms that the grain size increases with gamma irradiation. The optical parameters were estimated from optical transmission spectra data measured from a UV-vis-spectrophotometer in the wavelength range of 200-1100 nm. The refractive index dispersion data of the investigated thin films follow the single oscillator model. The estimated values of static refractive index n0, oscillator strength Ed, zero frequency dielectric constant ε0, optical conductivity σoptical and the dissipation factor increases after irradiation, while the single oscillator energy Eo decreases after irradiation. It was found that the value of the optical band gap of the investigated thin films decreases and the corresponding absorption coefficient increases continuously with an increase in the dose of gamma irradiation. This post irradiation changes in the values of optical band gap and absorption coefficient were interpreted in terms of the bond distribution model.

  4. Optimization of process parameters for the inactivation of Lactobacillus sporogenes in tomato paste with ultrasound and 60Co- γ irradiation using response surface methodology

    Science.gov (United States)

    Ye, Sheng-Ying; Qiu, Yuan-Xin; Song, Xian-Liang; Luo, Shu-Can

    2009-03-01

    The processing parameters for ultrasound and 60Co- γ irradiation were optimized for their ability to inactivate Lactobacillus sporogenes in tomato paste using a systematic experimental design based on response surface methodology. Ultrasonic power, ultrasonic processing time and irradiation dose were explored and a central composite rotation design was adopted as the experimental plan, and a least-squares regression model was obtained. The significant influential factors for the inactivation rate of L. sporogenes were obtained from the quadratic model and the t-test analyses for each process parameter. Confirmation of the experimental results indicated that the proposed model was reasonably accurate and could be used to describe the efficacy of the treatments for inactivating L. sporogenes within the limits of the factors studied. The optimized processing parameters were found to be an ultrasonic power of 120 W with a processing time of 25 min and an irradiation dose of 6.5 kGy. These were measured under the constraints of parameter limitation, based on the Monte Carlo searching method and the quadratic model of the response surface methodology, including the a/ b value of the Hunter color scale of tomato paste. Nevertheless, the ultrasound treatment prior to irradiation for the inactivation of L. sporogenes in tomato paste was unsuitable for reducing the irradiation dose.

  5. Optimization of process parameters for the inactivation of Lactobacillus sporogenes in tomato paste with ultrasound and {sup 60}Co-{gamma} irradiation using response surface methodology

    Energy Technology Data Exchange (ETDEWEB)

    Ye Shengying [College of Food Science, South China Agricultural University, Wushan, Guangzhou, GD 510640 (China)], E-mail: yesy@scau.edu.cn; Qiu Yuanxin; Song Xianliang; Luo Shucan [College of Food Science, South China Agricultural University, Wushan, Guangzhou, GD 510640 (China)

    2009-03-15

    The processing parameters for ultrasound and {sup 60}Co-{gamma} irradiation were optimized for their ability to inactivate Lactobacillus sporogenes in tomato paste using a systematic experimental design based on response surface methodology. Ultrasonic power, ultrasonic processing time and irradiation dose were explored and a central composite rotation design was adopted as the experimental plan, and a least-squares regression model was obtained. The significant influential factors for the inactivation rate of L. sporogenes were obtained from the quadratic model and the t-test analyses for each process parameter. Confirmation of the experimental results indicated that the proposed model was reasonably accurate and could be used to describe the efficacy of the treatments for inactivating L. sporogenes within the limits of the factors studied. The optimized processing parameters were found to be an ultrasonic power of 120 W with a processing time of 25 min and an irradiation dose of 6.5 kGy. These were measured under the constraints of parameter limitation, based on the Monte Carlo searching method and the quadratic model of the response surface methodology, including the a/b value of the Hunter color scale of tomato paste. Nevertheless, the ultrasound treatment prior to irradiation for the inactivation of L. sporogenes in tomato paste was unsuitable for reducing the irradiation dose.

  6. Effects of Pre-exposure of Mouse Pituitary with Low-dose 60Co γ-ray on Male Reproductive Endocrine Capacity Induced by Subsequent High-dose Irradiation

    Institute of Scientific and Technical Information of China (English)

    ZhangHong; LiWenjian; JingXiaodong; LiuBing; MinFengling; ZhouQingming; XieYi

    2003-01-01

    To investigate the effects of pre-exposure of mouse pituitary with low-dose 60Co γ-ray on male reproductive endocrine capacity induced by subsequent high-dose irradiation, the pituitary of the B6C3F1 hybrid strain male mice were irradiated with 0.05 Gy of 60Co γ-ray as the pre-exposure dose (D1), and were then irradiated with 2 Gy of 60Co γ-ray as challenging irradiation dose (D2) at 4 h after per-exposure. Pituitary gonadotroping hormones --follicle-stimulating hormone (FSH) and luterinzing hormone (LH), serum testosterone, testis weight and sperm count were measured at 35th day after irradiation.

  7. Preparation and Characterization of Fe3O4 Magnetic Nano-particles by 60Co γ-ray Irradiation

    Institute of Scientific and Technical Information of China (English)

    Mingcheng YANG; Hongyan SONG; Chengshen ZHU; Suqin HE; Ya GAO

    2007-01-01

    By using a new method, 60C0 γ-ray irradiation, Fe3O4 magnetic nano-particles were successfully synthesized at room temperature under ambient pressure. The structure, morphology and magnetic properties of these nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscope (TEM) and vibrating sample magnetometer (VSM), respectively. The radiation formation mechanism was also discussed. The results show that the absorbed dose can greatly influence the structure, morphology and magnetic properties of the products. XRD and TEM studies show that the product prepared by γ-ray irradiation (10 kGy) is pure FesO4 phase and the mean diameter of these nano-particles is about 21 nm. The Fe3O4 nano-particles synthesized by γ-ray irradiation (10 kGy) are mainly in small cubic shape and the size uniformity of these particles is good.

  8. Preliminary study on effects of 60Co γ-irradiation on video quality and the image de-noising methods

    International Nuclear Information System (INIS)

    There will be variable noises appear on images in video once the play device irradiated by γ-rays, so as to affect the image clarity. In order to eliminate the image noising, the affection mechanism of γ-irradiation on video-play device was studied in this paper and the methods to improve the image quality with both hardware and software were proposed by use of protection program and de-noising algorithm. The experimental results show that the scheme of video de-noising based on hardware and software can improve effectively the PSNR by 87.5 dB. (authors)

  9. Studies on progenitor cell compartments of 60Co irradiated and normal fetuses at 55 days of gestation

    International Nuclear Information System (INIS)

    Studies were performed on canine fetal tissues obtained from animals exposed to in vivo chronic irradiation and unexposed controls. CFU-GM, CFU-F and CFU-L assays were done using placental conditioned medium (PCM) or Concanavalin-A (Con-A) as the colony stimulating factor. Preliminary work using medium conditioned with canine spleen cells (SCM) was also begun which indicated that this colony stimulating factor greatly increases the number of colonies observed in both the CFU-GM and CFU-L assays. Chronic irradiation may adversely affect PCM responsive CFU-GM and CFU-F but not Con-A-reactive CFU-L

  10. Toxoplasma gondii gamma irradiation using Co-60; Irradiacao do Toxoplasma gondii com gama do {sup 60} Co

    Energy Technology Data Exchange (ETDEWEB)

    Serra-Freire, Nicolau Maues [Universidade Federal Rural do Rio de Janeiro, RJ (Brazil). Dept. de Parasitologia Animal; Maliska, Carmelindo [Hospital Central do Exercito, Rio de Janeiro, RJ (Brazil). Servico de Medicina Nuclear; Norberg, Antonio Neres [Instituto de Biologia do Exercito (IBEx), Rio de Janeiro, RJ (Brazil)

    1996-07-01

    The use of nuclear power through radiation for the destruction of microorganisms which cause food deterioration, infections and toxicosis, is specifically for peaceful purposes. Toxoplasma gondii is a protozoa responsible for illnesses in humans and animals. One of the most common ways of transmission is through raw or poorly cooked meat. There is little information on the resistance of T. gondii to radiation. The objective of this research is to determine the Minimum Lethal Dose (MLD) of gamma radiation for those microorganisms. Suspensions of T. gondii containing approximately one million taquizoites/ml were irradiated with doses between up 0,01 up to 0,15 kGy (Kilogray) and inoculated to mice. The surviving T. gondii were re-irradiated with 0,01 up to 0,16 kGy. The irradiated protozoa were totally destroyed with a 0,15 kGy dose (MLD). Taquizoites issued from live protozoa of 0,14 kGy also were completely destroyed with dose of 0,15 kGy. No increase in resistance was observed regarding the non irradiated protozoa. (author)

  11. 60Co γ-ray irradiation effect on germination and seedling growth of dry Buchloe dactyloides seeds

    International Nuclear Information System (INIS)

    The dry seeds of Buchloe dactyloides were irradiated by γ-ray at dose of 25-300 Gy. Seed germination and seedling characters were surveyed in laboratory and field. The results indicated that radiation could promote seed germination, and the optimum dose was 100 Gy. The dose of 150 Gy was the up limit to germination rate, root length and seedling height in field. When the radiation dose was bellow 100 Gy, the fresh weight of stems, leaves and roots of seedlings were increased. From this study, the recommended radiation does for Buchloe dactyloides dry seeds treatment was between 100-150 Gy for the purpose of promoting germination. (authors)

  12. Chemical changes in the chloroform-paraffin-dye system irradiated with 60Co gamma-rays, 2

    International Nuclear Information System (INIS)

    Studies have been carried out on the amount of hydrogen chloride formed by the radiolysis of chloroform in a solid paraffin-chloroform-Methyl Yellow system, and the color changes from yellow to red and absorption energy observed. The amount of hydrogen chloride formed and the intensity of the red color were determined with a pH meter, a spectrophotometer, and a color/ color-difference meter. It was found that the color-difference meter had the most excellent spectral response and sensitivity for measurement of the irradiated sample, and that the color-difference, ΔE, obtained by reflectometry increased proportionally with the increase in the radiation dose throughout a region of 100 -- 5000 R. When a solid sample composed of 1.0 kg paraffin (m.p. 62 -- 640C), 0.74 kg chloroform and 3.4 x 10-3 mol Methyl Yellow was subjected to 1000 R irradiation at 200C, 2.5 x 1014 molecules of hydrogen chloride were formed in 1.0 g of the solid sample with the absorption energy of 5.9 x 1016 eV, 3.2 x 1016 eV of which contributed to chloroform as the absorption energy. (author)

  13. Effects of 60CoIrradiation Intensity on Watermelon Seed Germination and Seedling Growth%60Co-γ辐射对西瓜种子萌发和幼苗生长的效应

    Institute of Scientific and Technical Information of China (English)

    李玉明; 杨世梅; 纪海波; 方春媛; 潘从祥; 陈年来

    2013-01-01

    In order to investigate the effects of different doses of 60Co-γ radiation on watermelon seed germination and seedling growth, watermelon seeds with different qualities of 12 varieties were used as the test materials to investigate the effects of six different doses of radiation on emergence rate, surviving rate, emergence time, cotyledon area, plant height and stem diameter. The results showed that medium size seeds of watermelon demonstrated a promoting effect on seedling growth under the dose of 200 Gy and 400 Gy. When the radiation dose exceed 600 Gy, all watermelon seeds showed the extremely significant level (P small seed > medium seeds.%为了探讨60Co-γ辐射剂量对不同质量西瓜种子萌发和幼苗生长的影响,以不同质量的12个西瓜品种干种子为材料,通过6个剂量的辐射处理,研究辐射剂量对西瓜干种子出苗率、存活率、出苗时间、子叶面积、株高及茎粗等的影响.结果表明,200和400 Gy辐射对中等种子出苗有促进作用,超过600 Gy都具有显著(P<0.05)抑制作用;随着辐射剂量的增加,植株出苗时间延长,幼苗生长缓慢;根据半致死剂量确定西瓜种子适宜辐射剂量为600~800 Gy.不同大小西瓜种子对60Co-γ辐射的敏感性表现为大种子>小种子>中等种子.

  14. Utilization of an elite rice germplasm-Yangdao 6 with high combining ability created by 60Co γ-irradiation

    International Nuclear Information System (INIS)

    Yangdao 6, a new rice variety bred by irradiation induced mutation, is an elite Indica germplasm with a higher general combining ability (GCA) and specific combining ability (SCA) compared with other restorer lines in yield composing traits, including total grains per panicle, filling grains per panicle, seed setting, 1000-grain weight, grain plumpness, yield per plant, etc. Used as a parent with high yield, good quality and multi-resistance, it has a good probability for breeding super-high-yield hybrid combinations both for the 'two-line' and Honglian-type CMS lines. And as a restorer line, it accelerates both the breeding of hybrid combinations and routine cultivars with a mode of 'boat goes up as river rises'. (authors)

  15. The influence of α-amylase supplementation, γ-irradiation (60Co) as well as long time of storage of wheat grain on flour technological properties

    International Nuclear Information System (INIS)

    The varieties of winter wheat, Aria and Beta, were studied. The Aria variety was stored for the period of four years. The part of wheat grain from Beta variety was irradiated with γ rays (60Co). In extracts from wheat kernels and flour protein content, total α- and β-amylolytic activity as well as α-amylolytic activity were determined, α-amylases of native and fungal origin were added to the flour obtained from samples of stored wheat kernels (Aria), irradiated and non-irradiated (Beta). Consequently native α-amylase activity of flour increased by 25% and 50%, respectively. Extensive technological estimation of grain and flour with amylase supplements was carried out. The study included: sedimentation analysis, falling number test, milling experiment, farinogram and extensogram analyses, measurement of the degree of damaged starch and flour colour, as well as baking experiment. The obtained experimental loaves of bread were tested for their ability to remain fresh. It was found out that the stored grain flour was characterized by the highest α-amylolytic activity and the lowest falling number value, whereas the irradiated grain flour showed the highest degree of starch damage and water absorption. When α-amylase supplementation to doughs was not accompanied by either irradiation or storage of grain, it definitely changed their physical properties for the worse. The negative influence of native α-amylases appeared to be less significant than that of fungal α-amylases. The positive influence of α-amylase supplementations, especially of those increasing by 25% the native α-amylolytic activity of flour on volume, and freshness of loaves of bread was observed. (author)

  16. Effects of Pre-exposure Mouse Pituitary with Low-dose 60Co γ-ray on Growth Hormone (GH) and Body Mass Induced by Subsequent High-dose Irradiation

    Institute of Scientific and Technical Information of China (English)

    ZhangHong; LiWenjian; JingXiaodong; LiuBing; MinFengling; ZhouQingming; XieYi

    2003-01-01

    The pituitary of the B6C3F1 hybrid strain mice were irradiated with 0.05 Gy of 60Co γ-ray as the pre-exposure dose (D1), and were then irradiated with 2 Gy of 60Co γ-ray as challenging irradiation dose (D2) at 4h after per-exposure. Body weight and serum growth hormone (GH) were measured at 35th day after irradiation. The results showed that irradiation of mouse testes with 2 Gy of 60Co γ-ray significantly diminished mousebody weight and level of serum GH (Table). Pre-exposure with a low-dose (0.05 Gy) of 60Co γ-ray significantly alleviated reductions of mouse body weight and level of serum GH induced by subsequent a high-dose (2 Gy) irradiation (Table). The data suggested that low-dose ionizing irradiation can induce adaptive responses to the harmful effects of pituitary by subsequent high-dose exposure.

  17. Toxoplasma gondii vs ionizing radiation: intestinal immunity induced in C57bl/6j mice by irradiated tachyzoites; Toxoplasma gondii vs radiacao ionizante: estudo da imunidade intestinal em camundongos C57Bl/6j experimentalmente vacinados com taquizoitos irradiados

    Energy Technology Data Exchange (ETDEWEB)

    Galisteo Junior, Andres Jimenez. E-mail: galisteo@usp.br

    2004-07-01

    We study the oral route for the development of a vaccine for toxoplasmosis, using parasites irradiated with 60 Cobalt, as an alternative for vaccine development to this worldwide parasitic infection. We evaluated the development of immunity at serum or mucosal levels, and their efficiency in protect the mice against challenge with oral cysts of the Me-49 strain. C57Bl/6j isogenic mice were immunized by oral route with 107 255 Gy irradiated tachyzoites from RH strain, at several protocols using milk as anti-peptic adjuvant and alum hydroxide as antacid. The preparations of irradiated tachyzoites induced production of serum IgG and IgA in immunized mice, as determined by ELISA, with IgG2a as the dominant subclass, similar to chronic infection. Their use with adjuvant allowed the excretion of significant amounts of IgA in stools also IgG, despite a lesser extent. There are suggestion of tolerance induction at mucosal level, with lower antigen induced proliferation and lower in vitro antibody production by spleen and gut lymphocytes, with the latter doses, specially when milk was used as adjuvant. All oral preparations induced some quantitative protection against challenge, which was similar to the parenteral route only isolated alum hydroxide was used as adjuvant. All these data support the possibility of the development of an oral vaccine against toxoplasmosis, using irradiated tachyzoites, which would be possible tool in near future for use in field baits, for immunizing either domestic or wild felines. (author)

  18. Suppressed cytokine and immunoglobulin secretions by murine splenic lymphocytes infected in vitro with Toxoplasma gondii tachyzoites.

    Science.gov (United States)

    Guk, S M; Kook, J; Jeon, Y H; Choi, J H; Han, E T; Shin, E H; Chai, J Y

    2005-04-01

    Mechanisms of host immunosuppression after infection with Toxoplasma gondii are unclear. This study was performed to observe cytokine and immunoglobulin secretions by murine splenic lymphocytes infected in vitro with live, nonreplicating (irradiated) RH tachyzoites on stimulation with concanavalin A (Con A) or lipopolysaccharide (LPS). For lymphocyte cultivation, 3 groups were prepared: coculture with live nonirradiated tachyzoites separated by a transwell (group T), live irradiated tachyzoites without a transwell (group R), and no tachyzoites (group C). Compared with group T, groups R and C, on stimulation with Con A, revealed significantly (P lymphocytes with T. gondii tachyzoites could impair their capacity to produce cytokine and immunoglobulin secretions.

  19. Tachyzoites of Toxoplasma gondii irradiated with 255 Gy induces decrease of cysts and cerebral lesions in mice challenged with cysts of ME-49

    International Nuclear Information System (INIS)

    Toxoplasmosis can cause ocular lesions in normal individuals and several diseases in foetus, HIV infection and transplants. Toxoplasma gondii has a complex life cycle, involving cats, as the definitive host, and warm blood species, as intermediated hosts. The infection occurs by ingestion of food and water contaminated with infected cat faeces, contaminated milk and cheese or raw and undercook meat of the intermediated hosts. To date, there is no commercial vaccine of use in humans. In this work, tachyzoites of T. gondii RH strain were irradiated with 255 Gy and inoculated in C57Bl/6j mice (3 doses, biweekly), after mice were challenged with 1, 5, 10, 20 and 25 cysts of ME-49 by oral gavage. The lesions and cysts in the brain were analyzed in all mice, after 4-week post infection. The mortality was 20% in control mice (ME-49 cysts only) and not one in immunized mice. The number of cysts was high in the control group, but low in immunized 255 Gy mice (n<100). Immunized mice showed less cerebral pathology and necrosis foci. Ionizing radiation is an important tool in the study toxoplasmosis and vaccine development. (author)

  20. Effects of gamma irradiation of the 60 Co on antimicrobial action of plant extracts of bark and leaves of Schinus terebinthifolius Raddi

    International Nuclear Information System (INIS)

    This study aimed to evaluate the potential for antimicrobial activity in vitro of extracts of bark and leaves of S. terebinthifolius treated with 60Co gamma radiation. 5,0 doses were used; 7.5 and 10 kGy, being held non-irradiated controls. To determine the antimicrobial activity was applied to the disc diffusion technique to evaluate the diameter of the inhibition zones against Gram-positive bacteria, gram-negative bacteria, alcohol-acid-resistant and yeast. Antimicrobial activity was considered significant for halos ≥ 15 mm. The results indicate an intensification of antimicrobial action of bark extracts, the 5.0 kGy, against S. aureus. Was held the micro dilution in broth to determine the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) of peels extracts, compared to eight clinical isolates of S. aureus. The MBC values showed that ionizing radiation did not produce the increased of anti bacteriostatic action of S. terebinthifolius, but the results indicated that S. terebinthifolius bark extracts can be used as an antimicrobial agent and ionizing radiation as an important alternative in this conservation feature

  1. 大剂量rhG-CSF早期单次给药对60Coγ射线照射小鼠的治疗作用%Therapeutic effects of early administration of a single high dose of rhG-CSF on mice irradiated by 60Coγ rays

    Institute of Scientific and Technical Information of China (English)

    韩阿如娜; 余祖胤; 柳晓兰; 从玉文

    2011-01-01

    Objective To observe the therapeutic effects of early administration with a single high dose of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on mice irradiated with 60Co γ rays, and provide a reference for the treatment of acute radiation syndrome (ARS) by using cytokines. Methods Male C57 mice underwent a total body irradiation of 8. Ogy 60Co y ray, and they were treated with rhG-CSF, at 0.5h and 24h, subcutaneously in a dose of 2, 1 and 0. 5mg/kg, respectively. The 30-day survival rate and mean survival time were observed in the lethal irradiated mice. The peripheral blood cell counts and bone marrow nucleated cell counts were evaluated in the sublethally irradiated mice. Results Early administration of a high dose of rhG-CSF significantly increased 30-day survival rate and prolonged mean survival time of mice with lethal irradiation dose. A single injection of rhG-CSF (lmg/kg) at 0. 5h after irradiation was an optimal administration schedule. In addition, early administration with a single high dose of rhG-CSF improved the recovery of bone marrow nucleated cell counts and peripheral blood counts, including white blood cell (WBC), red blood cell (RBC) and platelet in mice exposed to 6. Ogy irradiation. Conclusion Early administration of a single high dose rhG-CSF may have a favorable therapeutic effect on mice irradiated with 60Co y ray.%目的 观察大剂量rhG-CSF早期单次给药对60Coγ射线照射小鼠的治疗作用,为细胞因子治疗急性放射病提供实验依据.方法 雄性C57小鼠,经8.0Gy 60Co γ射线全身照射后于0.5、24h各皮下注射一次不同剂量rhG-CSF(2、1mg/kg和0.5mg/kg),观察致死剂量照射小鼠的30d存活率及平均生存时间.小鼠经6.0Gy 60Co γ射线全身照射后,通过不同给药方案及不同剂量rhG-CSF早期干预,观察亚致死剂量照射小鼠的外周血象和骨髓有核细胞数的变化.结果 大剂量rhG-CSF早期干预明显提高致死剂量照射小鼠的30d存

  2. Differential radio-sensitivities of human chromosomes 1 and 2 in one donor in interphase- and metaphase-spreads after 60Co γ-irradiation

    International Nuclear Information System (INIS)

    Radiation-induced chromosome aberrations lead to a plethora of detrimental effects at cellular level. Chromosome aberrations provide broad spectrum of information ranging from probability of malignant transformation to assessment of absorbed dose. Studies mapping differences in radiation sensitivities between human chromosomes are seldom undertaken. Consequently, health risk assessment based on radio-sensitivities of individual chromosomes may be erroneous. Our efforts in this article, attempt to demonstrate differences in radio-sensitivities of human chromosome-1 and/or -2, both in interphase and metaphase spreads. Upon blood collection, dosimetry and irradiation were performed. Lymphocytes were isolated after whole-blood irradiation with 60Co γ-rays in the dose range of 0–5 Gy for both interphase, and metaphase aberration studies. Induction of premature chromosome condensation in interphase cells was accomplished using a phosphatase inhibitor, calyculin-A. Metaphase spreads were harvested from short-term peripheral blood lymphocyte cultures following colcemid arrest and using an automated metaphase harvester and spreader. Aberration analysis in both interphase and metaphase spreads were done using FISH. In interphase, aberrant cell and aberration frequency involving chromosome 1 and/or 2 increased linearly with radiation dose. In metaphase, aberrations increased in a linear-quadratic manner with dose. Our studies ascertain that chromosome-2 is more radio-sensitive than chromosome-1 in both interphase and metaphase stages, albeit the DNA content of chromosome-2 is lesser than chromosome-1 by almost 10 million base pairs. Differences in radio-sensitivities of chromosomes have implications in genetic damage, chromosome organization, and chromosome function. Designing research experiments based on our vital findings may bring benefit to radiation-induced risk assessment, therapeutics and development of chromosome specific biomarkers

  3. Characterization of {sup 60}Co and {sup 137}Cs beams from irradiator Shepherd 81 - 14D of LNMRI/IRD in radiation protection; Caracterizacao dos feixes de {sup 60}Co and {sup 137}Cs do Irradiador Shepherd 81 - 14D do LNMRI/IRD em radioprotecao

    Energy Technology Data Exchange (ETDEWEB)

    Silva, C.N.M. da; Cabral, T.S., E-mail: cosme@ird.gov.br [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

    2015-07-01

    The objective of this work is the characterization of {sup 60}Co beams and the {sup 137}Cs irradiator Shepherd 81-14D acquired by the National Metrology Laboratory of Ionising Radiation of the Institute of Radiation Protection and Dosimetry (LNMRI /IRD), for use in calibration of measuring instruments of gamma radiation in air kerma and ambient dose equivalent, irradiation of films and TLD's. Field homogeneity measurements were carried out, calculated input and output time of the source and commissioning of sources. (author)

  4. Evaluation of the radio modifier effect of propolis on chinese hamster ovary (CHO-K1) and human prostate cancer (PC3) cells, irradiated with 60-CO

    International Nuclear Information System (INIS)

    In the last decades, it has been given a great interest to investigations concerning natural, effective, nontoxic compounds with radioprotective potential together with the increasing utilization of different types of ionizing radiation for various applications. Among them propolis, a resinous compound produced by honeybees (Apis mellifera), has been considered quite promising, since it presents several advantageous biological characteristics, i. e., anti-inflammatory, antimicrobial, anticarcinogenic, antioxidant and also free radical scavenging action. The purpose of the present study was to evaluate the effect of Brazilian propolis, collected in the State of Rio Grande do Sul, on Chinese hamster ovary (CHO-K1) and human prostate cancer (PC3) cells, irradiated with 60Co γ radiation. For this purpose, three interlinked parameters were analyzed: micronucleus induction, cell viability and clonogenic death. The choice of these parameters was justified by their biological significance, in addition to the fact that they are readily observable and measurable in irradiated cells. The cytogenetic data obtained showed a radioprotective effect of propolis (5-100 μg/ml) in the induction of DNA damage for both cell lines, irradiated with doses of 1 - 4 Gy. The cytotoxicity assay, however, showed a prominent antiproliferative effect of propolis (50 - 400μ/ml) in PC3 cells irradiated with 5 Gγ. The survival curves obtained were adequately fitted by a linear-quadratic model, where the α coefficient was higher in CHO-K1 cells. Concerning the clonogenic capacity, PC3 cells were more radiosensitive than CHO-K1 cells at the higher doses of the survival curve. Propolis at the concentrations of 30 - 100 μg/ml, did not influence the clonogenic potential of PC3 cells, since the survival curves, associated or not with propolis, were found similar, although the combined treatment in CHO-K1 cells exhibited a stimulating proliferative effect. The data obtained in vitro showed a

  5. Effect of sodium alginate coating and 60Coirradiation treatment on fresh-keeping of golden silk jujube%海藻酸钠涂膜及60Co-γ辐照处理对小枣的保鲜作用

    Institute of Scientific and Technical Information of China (English)

    杨伟; 徐莹; 樊燕; 汪东风; 孙寿魁

    2012-01-01

    Using golden silk jujube at full-red storage as materials,the effects of different preservation treatments(60Coirradiation,coating with sodium alginate,and 60Co-γ irradiation+coating with sodium alginate,respectively) on the post-harvest fruit quality during cold storage were investigated,and the weight loss rate,total sugar content,reducing sugar content,VC content,and ratio of sugar to acid were determined.The results indicated that 60Coirradiation,coating with sodium alginate and 60Co-γ irradiation+coating with sodium alginate all had an obvious fresh-keeping effect.The result showed that the three treatments reduced water loss,delayed the decrease of VC and titratable acid content,and the increase of sugar to acid ratio,and 60Co-γ irradiation+coating with sodium alginate maintained a relatively high content of total sugar and reducing sugar.Especially,the preservative effect of the 60Co-γ irradiation+coating with sodium alginate treatment was obviously superior to the control group,60Coirradiation or coating with sodium alginate alone,which could obviously improve the quality of post-harvest cold storage of golden silk jujube.%以全红期的山东省乐陵小枣为实验材料,分别进行60Co-γ辐照处理、海藻酸钠涂膜处理及二者结合处理后,与对照组同时于(2±0.5)℃冷库贮藏,并对低温贮藏过程中枣的失重率、总糖、还原糖、VC、可滴定酸含量及糖酸比的变化进行测定。结果表明:涂膜、辐照、涂膜+辐照处理均能有效减少水分散失,延缓VC和可滴定酸的下降,抑制糖酸比的上升,涂膜+辐照处理能保持较高的总糖、还原糖含量。其中,60Co-γ辐照+海藻酸钠涂膜处理于(2±0.5)℃冷库贮藏保鲜效果显著优于其他实验处理组,有助于提高乐陵小枣采后的低温贮藏品质。

  6. Cytotoxicity evaluation of Diethyltoluamide (DEET) in Perna perna (Linnaeus, 1758) mussels non-irradiated and irradiated with {sup 60}Co gamma radiation; Avaliacao da citotoxicidade do dietiltoluamida (DEET) em mexilhoes Perna perna (Linnaeus, 1758) irradiados e nao irradiados com radiacao gama de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Martini, Gisela de Assis

    2013-07-01

    Recent studies have identified the presence of several emerging pollutants in aquatic environments. The occurrence in different environmental matrices has been continuously reported, highlighting the need for toxicity studies. The DEET (N,N-diethyl-meta-toluamide) is the active ingredient used in most insect repellents, and is present in many commercially available formulations. Apart from chemical pollutants, aquatic organisms are subject to exposure of ionizing radiation from natural sources or in the vicinity of nuclear power plants. The present study evaluated the toxicity of DEET in organisms irradiated and non-irradiated with {sup 60}Co gamma radiation, and the effects that radiation causes in lysosomes of hemocytes of Perna perna mussel. For this purpose, assays were performed to identify the acute toxicity of DEET concentration and the dose of gamma radiation able to cause mortality. Subsequently, cytotoxicity assays were carried out to assess the stability of the lysosomal membrane in organisms exposed to ionizing radiation and DEET. According to the results obtained in acute toxicity tests, the concentration of DEET that causes mortality of 50% exposed organisms (LC50) is 114,27 mg L{sup -1}, and the radiation dose that causes mortality (LD50) is 1068 Gy. In the cytotoxicity assays, the concentration of the non-observed effect (NOEC) for irradiated and non-irradiated organisms 0.0001 mg L-1 and observed effect concentration (LOEC) at concentrations above this. The IC25 (72h) for non-irradiated organisms was 0.0003 mg L-1 and IC50 (72h) was 0.0008 mg L{sup -1} for irradiated and non-irradiated organisms. Despite of the concentrations of effect found in this study were higher than in the environment, both measurements are in the same order of magnitude and should be also take into account the possible synergistic effects of DEET with other contaminants in the aquatic environment. (author)

  7. Effect of 60Co γ-ray Irradiation on Growth Characteristics of Chamaecrista%60Co-γ射线辐照处理对决明若干生长特性的影响

    Institute of Scientific and Technical Information of China (English)

    翁伯琦; 徐国忠; 郑向丽; 应朝阳; 黄毅斌

    2005-01-01

    利用60℃o-γ射线(辐照剂量为300 Gy,剂量率为4.687 5 Gy/min)辐照豆科决明属5个牧草品种的种子,通过盆栽收集各个品种的M1代种子,然后进行M2代试验与观察.结果表明,辐照后5个决明M2代的种子出苗率、植株的茎长、叶宽均无显著差异;34721号决明植株冠高有显著增长、2219号决明茎径有显著增长、86134号决明分枝数有极显著增长,92985号决明分枝数则有显著降低、86134号决明叶数和地上部植株干重有显著增长、2219号决明叶长增长显著,其余参试品种则无显著差异.植株的营养成分测定表明:仅2219号决明K含量呈显著下降、其他品种的N,P,K含量则无显著差异;2219不仅氨基酸总量呈显著下降、而且17种氨基酸都表现为不同程度的下降,86134氨基酸总量有显著增长、同时17种氨基酸都表现为不同程度的增长,而其余3个参试品种都无显著差异.经60Co-γ射线辐照处理后的34721,86134,92985的酯酶同工酶谱与相应的对照有明显差别.生长观察结果表明,参试的5个决明品种M2代植株生长的现蕾期、初花期、盛花期、初荚期、盛荚期、成熟期表现为双向性,既有提早,也有推迟.

  8. Resveratrol Effects on Life Span and Fertility of Caenorhabditis Elegans Subject to 60Co Gamma Ray Irradiation%白藜芦醇对60Coγ射线照射线虫寿命和生殖的影响

    Institute of Scientific and Technical Information of China (English)

    叶侃; 季晨博; 郭锡熔; 古桂雄

    2011-01-01

    Caenorhabditis elegans was used as experimental model to investigate radiation effect of resveratol on caenorhabdities elegans irradiated by 60Co γray. Treatment with resveratrol can increase average life span and spawning rate, improve the surrival rate of eggs, and protect their mitochondrion function of cacnorhabdities elegans exposure to 60Co rr ray. The results indicate that resveratrol has radiation protection effects, which might be related to its action on ROS decrease and mitochondrial defend.%以线虫作为实验模型,观察白藜芦醇(RES)对受60Coγ射线照射线虫的保护作用.结果表明,白藜芦醇能提高受照射线虫的寿命,增加产卵率,提高卵的存活率,保护其线粒体功能.其作用机制可能与白藜芦醇减轻氧自由基所致的线虫线粒体损伤有关.

  9. 6Co γ射线诱发小鼠DNA甲基化改变%DNA methylation changes induced by 60 Co γ-ray irradiation

    Institute of Scientific and Technical Information of China (English)

    黄铖铖; 孙秀锦; 胡明江; 程跃进; 古桂雄; 崔凤梅

    2011-01-01

    Objective To study the change and significance of DNA methylation pattern and DNA methyltransferase (DNMT1) protein in 60Co γ-ray irradiated mice tissues. Methods Twelve SPF C57BL/6J mice were randomly divided in-tocontrol group and radiation group. The radiation group were exposed to 4Gy-single whole body radiation. All mice were sacrificed 3 days after the radiation. The total number of peripheral white blood cells and the frequencies of micronucleated polychromatic erythocytes(fMNPCE) were measured. Methylated DNA immunoprecipitation-chip(MeDIP-chip) was adopted to investigate the differentially expressed CpG islands methylation profiles in mouse liver genome promoter. The immunohisto-chemical(SP method) and western blot method were used to check the expression of DNMT1 protein in tissues. Results Compared with the control group,the promoter methylation profiles of irradiated mice changed obviously, with 1300 genes methylated and 660 genes demethylated. With the improvement of the screening criteria,the obviously methylated genes were Trim71,Sema3f, Pcdh8,Sox4,1110034A24Rik, Limkl, Nefl, and Xprl and the demethylated genes were Sfrsl8,Drl, Ankrd42,Nael ,Sfil ,Accnl ,Srd5al ,Nsun2,Eifla,and Dusp5. Except in the lung,the expression of DNMT1 protein in liver, kidney, testicle, and brain tissues were all increased (P < 0.05). Conclusion There are methylation changes of gene promoter in ionizing radiation injury and the increased expression of DNMT1 may be the reason of gene promoter methylation.%目的 探讨基因组启动子甲基化谱及DNA甲基化酶1(DNMT1)表达量在60Co-γ射线照射小鼠各组织的变化及其意义.方法SPF级C57BL/6J雄性小鼠12只随机分为对照组和照射组,照射组小鼠接受4Gy60 Co-y射线单次全身照射后,均眼球摘除取血后处死,收集各脏器组织,进行外周血白细胞计数和骨髓嗜多染红细胞微核计数,应用甲基化DNA免疫沉淀-芯片(MeDIP-chip)杂交技术对y射线

  10. 60Co放射及微量元素锌对钛种植体骨融合的影响%Effect of60Co irradiation and trace element zinc on implant-bone fusion

    Institute of Scientific and Technical Information of China (English)

    陈海军; 张安生; 朱孝春; 钱奇春; 于淑湘; 杨博程; 张静波; 南福清; 林均舟

    2015-01-01

    BACKGROUND:Zinc supplementation can accelerate implant-bone fusion. OBJECTIVE:To observe the effect of 60Co irradiation and trace element zinc on implant-bone fusion. METHODS:A total of 36 adult male rabbits were involved. One piece of titanium implant was placed into the proximal end of the rabbit's bilateral tibial heads respectively to set up the animal model with titanium implants. Then the animals were randomly divided into four groups. Twenty-four hours after implantation, 10 g/L zinc sulfate was administered intramuscularly to the animals in the zinc supplement group at the dose of 4 mg/kg, once per day; 9 g/L normal saline was administered intramuscularly to the animals in the control group at the dose of 4 mg/kg, once per day. The animals in the 60Co irradiation group received 60Co irradiation at 2, 4, 6 days at the dose of 15 Gy per day, and 24 hours after implantation, 9 g/L normal saline was administered intramuscularly at the dose of 4 mg/kg, once per day; while those in the 60Co irradiation and zinc supplement group received 60Co irradiation at 2, 4, 6 days at the dose of 15 Gy per day, and 24 hours after implantation, 10 g/L zinc sulfate was administered intramuscularly to the animals at the dose of 4 mg/kg, once per day. The animals were kiled at 1, 4, 12 weeks after treatment. Stereomicroscope was used to observe the histomorphology on the implant-bone interface. RESULTS AND CONCLUSION:Compared to other groups, at the same time, more fibroblasts and fibrous fusion were observed around the implants from the 60Co irradiation group while less bone tissue, especialy mature bone tissue, was observed. On the contrary, at the same time, the surfaces of the implants from the zinc supplement group showed more osteoblasts and bone fusion. Mature bone tissue was observed around the implants at the 4th week after implantation indicating that zinc supplement can accelerate the new bone formation on the implant-bone interface at 1-4 weeks after treatment to

  11. Activity evaluation from different native or irradiated with {sup 60} Co gamma rays snake venoms and their inhibitory effect on Leishmania (Leishmania) amazonensis; Avaliacao da atividade de diferentes venenos de serpentes, nativos ou irradiados, com radiacao gama de {sup 60} Co, quanto ao poder inibitorio do crescimento de Leishmania (Leishmania) amazonensis

    Energy Technology Data Exchange (ETDEWEB)

    Lourenco, Cecilia de Oliveira

    2000-07-01

    Cutaneous leishmaniasis is a disease, caused by Leishmania parasites, that occurs frequently in tropical and sub-tropical regions of the world. Skin lesions that could results in disfiguring aspect characterize it. The treatment is based on few drugs as antimony salts or pentamidine that are toxic with increasing resistance by the parasite. Alternative forms of disease treatment are in constant search, including natural components as snake venoms. Previous studies demonstrate that some components of snake venoms have an inhibitory effect against those parasites, including Leishmania species. Although snake venoms presented high toxicity, several methods have been described to detoxify most or some of their toxic components, with favorable results by the use of gamma irradiation. In this report we tested several native and irradiated snake venoms for inhibitory effect against Leishmania (Leishmania) amazonensis parasite and LLCMK{sub 2} mammalian cells, with enzymatic tests and electrophoresis. There are significant activity in Acanthophis antarcticus, Agkistrodon bilineatus, Bothrops moojeni, Bothrops jararaca, Hoplocephalus stephensi, Naja melanoleuca, Naja mossambica, Pseudechis australis, Pseudechis colletti, Pseudechis guttatus and Pseudechis porphyriacus, venom being inactive Pseudonaja textilis, Notechis ater niger, Notechis scutatus. Oxyuranus microlepidotus and Oxyuranus scutellatus venoms. After 2 KGy of {sup 60}Co irradiation most venom loses significantly their activity. Venoms with antileishmanial activity presented L-amino acid oxidase (L-AO) activity and showed common protein with a molecular weight about 60kDa in SDS-PAGE. These results indicate that L-AO activity in those venoms are probably related with antileishmanial effect. (author)

  12. Effect of dose-rate of gamma irradiation (60Co) on the anti nutritional compounds phytic acid and antitrypsin on soybean (glycine max L.)

    International Nuclear Information System (INIS)

    An investigation on the effect of gamma irradiation at different dose-rate on the anti-nutritional compounds (phytic acid and antitrypsin) and the color of soybean has been conducted. The purpose of the study was to analyze the influence of the dose-rate on the rate of change of anti-nutritional compounds and color. Samples were irradiated with dose-rates of 1.30; 3.17; 5.71 and 8.82 kGy/hour with irradiation time varied from 0.5 to 55 hours. Phytic acid content and antitrypsin activity, as well as their L α b color values were analyzed. Results showed that a simple first order kinetics model can be used to describe changes in the concentration of the anti-nutritional compounds and color soybeans during the radiation processing. Data indicate that irradiation process at higher dose-rate (shorter time) is more effective in destroying anti-nutritional compounds as compared to that of irradiation process at lower dose-rate (longer time). Furthermore, irradiation process at higher dose-rate (shorter time) also have less detrimental effect on color of the soybean and the resulted soybean flour as compared to that of irradiation process at lower dose-rate (longer time). These findings suggest that irradiation process at a same dose may potentially be optimized by selecting the most appropriate combination of dose-rate and time of irradiation. (author)

  13. Influence of natural antioxidants on lipid composition of beef burgers submitted to irradiation in {sup 60} Co source and electron beams; Influencia de antioxidantes naturais sobre o perfil lipidico de hamburgueres bovinos submetidos a irradiacao por {sup 60}Co e aceleradores de eletrons

    Energy Technology Data Exchange (ETDEWEB)

    Trindade, Reginaldo Almeida da

    2007-07-01

    Radiation processing has been employed in some countries as a mean of treatment to assure microbiological safety of meat and meat products, avoiding the occurrence of food-borne disease. The ionizing radiation may cause some undesirable changes on chemistry composition of food and the lipid oxidation is one of the main reactions. In meat products processing industry, the lipid composition is directly related to nutritional and sensory quality of the product. For preventing oxidation, use of antioxidants which can be synthetic or natural, has been practically applied in some products. Currently, most attention has been given to natural antioxidants from herbs and spices like rosemary and oregano. The aim this study was to assess the antioxidant effects of either rosemary and oregano extract in beef burgers submitted to irradiation in {sup 60}Co source with dose 6, 7 e 8 kGy, electron beams with dose 3,5 e 7 kGy and storage under freeze along 0, 45 e 90 days. The results showed that rosemary extract has the major antioxidant effects when it is used on heterogeneous food matrix like beef burger, but oregano extract was better efficient to delay lipid oxidation along storage time when it is used in synergism with rosemary and/or BHT/BHA. Although to have occurred changes in the fatty acids composition it was not possible to demonstrate a straight dependence of irradiation dose and/or storage time. Sensory analysis showed that between the samples prepared with natural antioxidants, the beef burger prepared with oregano has received better scores by panelists. Irradiated beef burger prepared with rosemary has received better scores when compared to non-irradiated one. The use of spices with antioxidant activity to avoid the oxidative damage in foods that contain fats in their formulation is thought to be promising to application in food facilities. (author)

  14. Microstructural study of Y1Ba2Cu3O7-x/Ag samples irradiated with 60Co γ rays at high doses

    International Nuclear Information System (INIS)

    We have investigated the damage induced by irradiation in Y1Ba2Cu3O7-x silver added samples. The samples were prepared with 0 and 6.5 wt% of silver and irradiated by high-energy γ irradiation (50-150 Mrad). The roles of silver and dosage irradiation are discussed in terms of their effects on microstructure, crystallinity, critical temperature (Tc) and zero-resistance temperature (T0). After irradiation, Tc decreased while the room-temperature electrical resistance increased by a factor of 8 for some of the samples. The difference in T0 between irradiated and non-irradiated YBCO samples was of the order of 10 K. We have found that the difference is bigger for silver-added samples. We have also observed several changes in diffraction patterns of YBCO and YBCO-silver samples. SEM images, EDS and XPS analysis showed that silver resided inside the grains as single atoms and as metallic clusters. The relative concentrations of the elements in samples were quantified by Auger electron spectroscopy. The values showed a gradual increase for radiation doses ranging between 0 and 100 Mrad. For doses up to 100 Mrad, Jc decreased because of the weak-link breakage induced by high doses of γ rays. (author)

  15. Study on the grafting of N-vinypyrrolidone onto xanthan gum by 60Co γ-irradiation and properties of the grafted copolymer

    International Nuclear Information System (INIS)

    Simultaneous radiation grafting method (60Co γ-rays as the radiation source) was used to prepare xanthan gum-graft-N-vinypyrrolidone (XG-g-NVP), and the influence factors of the grafting degree was preliminarily studied. The structure of the grafted copolymer was characterized by the Fourier-transform infrared spectroscopy (FT-IR) and thermo gravimetric analysis (TGA), and the solution viscosity properties of the grafted copolymer in low grafting rate were studied by rheometer. The results show that the grafting rate increases with absorbed dose and gradually achieve to the balance. The grafting rate reaches maximum when the xanthan gum concentration is 10 g/L or the mass ratio is 10:1 meanwhile the other two factors are in a certain experimental value range. FT-IR figure shows that the grafting reaction occurs in NVP vinyl part and lactam structure is retained. Thermo-gravimetric analysis shows that XG-g-NVP enhanced thermal stability of xanthan gum. The properties of XG-g-NVP solution show that grafting to enhance the performance of resisting high temperature and shear strength. (authors)

  16. 60Coγ射线半身照射对非照射区域骨髓造血组织基质细胞衍生因子1表达的影响%Influences of 60Coγray irradiation on expression stromal cell derived factor-1 in bone marrow hematopoietic tissue of non-irradiation area in left-half-body ionizing irradiated mice

    Institute of Scientific and Technical Information of China (English)

    高作文; 杨龙; 陈乐如; 娄金书; 张国强; 李开信; 程天民

    2013-01-01

    Objective To invesligale the mouse bone marrow hemalopoielic functions in non-irradiation area after irradiated by way of left-half- body. Methods The 6-8-week male Kunming strain mice were randomly divided into normal control( NC) , total-body-irradiated( TBI) , left-half-body-irradiated( LHBI) , and total-body-shield-irradia-ted( TBSI) groups. Left-half-body-irradiated group was treated with two pieces of 5 cm x 8 cm x 16 cm overlapped lead bricks shielding right-side body and irradiated with 8. 0 Gy60Coγ-ray. The leukocyte in peripheral blood and the number of bone marrow hematopoietic cells( BMHCs) were studied, the concentration of SOD, MDA in mouse serum were measured, and the expression SDF-1 in bone marrow hematopoietic tissues were observed by the Western blotting method and laser scanning confocal microscope combined with immunohistochemistry. Results In the left-half-body irradiated condition, the leucocyte in peripheral blood and the BMHCs were diminished, the concentration of MDA was increased and the SOD was decreased in the mouse serum remarkably ( compared with NC, P <0. 01) ; In non-irradiation area, the SDF-1-positive cells and the expression SDF-1 in bone marrow hematopoietic tissues were reduced significantly. Conclusion Our study suggested that the local irradiation resulted in the decrease of SDF-1-positive cells and the decline expression SDF-1 in bone marrow hematopoietic tissues in non-irradiation area, and the increase of reactive oxygen or free radicals might play an important role in the abnormal expression of SDF-1 in BMHT and the injury of hematopoietic microenvironment.%目的 探讨局部电离辐射对小鼠非照射区域骨髓造血组织基质细胞衍生因子-1(SDF-1)表达的影响.方法 将6~8周龄雄性昆明小鼠随机分为健康对照组、全身照射组、全身屏蔽照射组以及左半身照射组4组,用铅屏蔽建立半身照射模型,以8.0 Gy 60Co γ射线照射,观察小鼠外周血白细胞和骨髓有核

  17. Effects of gamma irradiation of the {sup 60} Co on antimicrobial action of plant extracts of bark and leaves of Schinus terebinthifolius Raddi; Efeitos da radiacao gama do {sup 60} Co sobre a acao antimicrobiana de extratos vegetais de cascas e folhas de Schinus terebinthifolius Raddi

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Edvane Borges da; Santos, Gustavo Henrique Farias dos; Amaral, Ademir de Jesus [Universidade Federal da Pernambuco (GERAR/DEN/UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear. Grupo de Estudos em Radioprotecao e Radioecologia; Lima, Jeniffer Maiza de Souza [Universidade Federal da Pernambuco (CAV/UFPE), Vitoria de Santo Antao, PE (Brazil); Xisto, Kesia; Araujo, Rosilma de Oliveira [Universidade Federal da Pernambuco (DA/UFPE), Recife, PE (Brazil). Dept. de Antibioticos

    2014-07-01

    This study aimed to evaluate the potential for antimicrobial activity in vitro of extracts of bark and leaves of S. terebinthifolius treated with {sup 60}Co gamma radiation. 5,0 doses were used; 7.5 and 10 kGy, being held non-irradiated controls. To determine the antimicrobial activity was applied to the disc diffusion technique to evaluate the diameter of the inhibition zones against Gram-positive bacteria, gram-negative bacteria, alcohol-acid-resistant and yeast. Antimicrobial activity was considered significant for halos ≥ 15 mm. The results indicate an intensification of antimicrobial action of bark extracts, the 5.0 kGy, against S. aureus. Was held the micro dilution in broth to determine the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) of peels extracts, compared to eight clinical isolates of S. aureus. The MBC values showed that ionizing radiation did not produce the increased of anti bacteriostatic action of S. terebinthifolius, but the results indicated that S. terebinthifolius bark extracts can be used as an antimicrobial agent and ionizing radiation as an important alternative in this conservation feature.

  18. Study of the effect of gamma irradiation (60 Co) on the quality of sugar cane spirit and on the cask of maturation

    International Nuclear Information System (INIS)

    The ageing or maturing process of drink improves the sensorial characteristics of the product making it a qualified drink with higher economical value. The traditional maturing method of drinks involves their interaction with wood and irradiation can accelerate this ageing process. Sugar cane spirit and oak barrels with a 20 L capacity were treated using gamma irradiation (150 Gy). Physical-chemical and chromatographic analyses were constantly performed for 390 days during the drink ageing period. The sugar cane spirit and barrel irradiation did not change most volatile components of the same type coefficient such as volatile acidity, esters, superior alcohols and furfural throughout the 390 days. There is evidence, however, that some component parameters like aldehydes, tannin, color and copper concentration are in some way influenced resulting in partial acceleration of the ageing or maturing process. At the end of the ageing period, a sensorial analysis was made using 30 non-trained people who tasted the drink. The ageing process acceleration was confirmed by the sensorial evaluation, where the irradiated sugar cane spirit and/or barrel had higher approval regarding all parameters analyzed (scent, flavor and appearance). (author)

  19. Study of the effect of gamma irradiation (60CO) on the quality of sugar cane spirit and on the cask of maturation

    International Nuclear Information System (INIS)

    The ageing or maturing process of drink improves the sensorial characteristics of the product making it a qualified drink with higher economical value. The traditional maturing method of drinks involves their interaction with wood and irradiation can accelerate this ageing process. Sugar cane spirit and oak barrels with a 20 L capacity were treated using gamma irradiation (150 Gy). Physical-chemical and chromatographic analyses were constantly performed for 390 days during the drink ageing period. The sugar cane spirit and barrel irradiation did not change most volatile components of the same type coefficient such as volatile acidity, esters, superior alcohols and furfural throughout the 390 days. There is evidence, however, that some component parameters like aldehydes, tannin, color and copper concentration are in some way influenced resulting in partial acceleration of the ageing or maturing process. At the end of the ageing period, a sensorial analysis was made using 30 non-trained people who tasted the drink. The ageing process acceleration was confirmed by the sensorial evaluation, where the irradiated sugar cane spirit and/or barrel had higher approval regarding all parameters analyzed (scent, flavor and appearance)

  20. 60Co γ射线辐照预处理对甘薯热风干燥特性的影响%INFLUENCE OF 60Co γ IRRADIATION PRE-TREATMENT ON CHARACTERISTICS OF HOT AIR DRYING SWEET POTATO SLICES

    Institute of Scientific and Technical Information of China (English)

    江宁; 刘春泉; 李大婧; 刘霞; 严启梅

    2012-01-01

    The influences of irradiation, hot air temperature and thicknesses of the slices on the characters of dehydration and surface temperature of 60Co γ-rays irradiated sweet potato were investigated. Meanwhile, microscopic observation and determination of water activity of irradiated sweet potato were conducted. The results show that the drying rate and the surface temperature rised with the increasing of irradiation dose. When the dry basis moisture content was 150% , the drying rate of the samples were 1.92, 1.97, 2.05, 2.28, 3.12%/min while the irradiation dose were 0, 2, 5, 8, 10kGy, and the surface temperature were 48.5℃ , 46.3℃ , 44.5℃, 42.2℃, 41.5℃ , respectively. With higher air temperature and thinner of the sweet potato slices, the dehydration of the irradiated sweet potato slices were faster. The drying speed of sweet potato slices at 85℃ was 170min faster than that of 65℃. The drying speed of 7mm sweet potato slices was 228min faster than that of 3mm sample. The cell wall and the vacuole of the sweet potato slices were broken after irradiation, and its water activity increased with the inorease is radiation dose. The water activity of the irradiated samples were 0. 92, 0. 945, 0. 958, 0. 969, 0. 979 with the irradiation doses of 0, 2, 5, 8, 10kGy, respectively. The hot air drying rate, surface temperature and water activity of sweet potato are significantly impacted by irradiation. The conclusion provids a theoretical foundation for further processing techonology of combined radiation and hot air drying sweet potato.%采用60Coγ射线对甘薯进行辐照预处理,考察辐照、热风温度和切片厚度对其干燥特性和表面温度的影响,同时对不同剂量辐照的甘薯样品进行了显微观察和水分活度测定。结果表明,甘薯的干燥速率和表面温度随着辐照剂量的升高而升高。当干基含水率为150%时,辐照剂量为0、2、5、8和10kGy的样品干燥速率分别为1.92、1.97、2.05、2.28和3

  1. Research of Effect of 60Co γ-ray Irradiation on Cell Cycle of SMMC, 7721 Tumor Cell with Flow Cytometery

    Institute of Scientific and Technical Information of China (English)

    DangBingrong; MaQiufeng; GaoQingxiang; LiWenjian; HaoJifang; XieYi; GuoChuanling

    2003-01-01

    The resultsMost researchof cell cycle play an important role in resear, ching tumor occurrence, development and treatment. results show that malignant grade and pharmic sensitivity of tumor are relative to cell cycle. The sensitivity of medications is different to different phases of cell cycle of tumor. In general, the cell of M are more sensitivity. On the side, different medications have different action in different cell cycle. The irradiation can change cell cycle proccss and can induce the pattern of changes in cell cycle. For cxamplc, G1 arrest, G2 arrest and S arrest. So, thc research rcsults of tumor cell cycle in different irradiation have not only biological means but also realistic means for selecting chemical therapy medication after radiotherapy.

  2. Thermal stability of formulations of PVC irradiated with {gamma} of {sup 60}; Estabilidad termica de formulaciones de PVC irradiadas con {gamma} de {sup 60} Co

    Energy Technology Data Exchange (ETDEWEB)

    Martinez P, M.E.; Carrasco A, H. [ININ, A.P. 18-1027, 11801 Mexico D.F. (Mexico)]. E-mail: memp@nuclear.inin.mx; Castaneda F, A.; Benavides C, R.; Garcia R, S.P. [CIQA, 25100 Saltillo, Coahuila (Mexico)

    2004-07-01

    The industry of cables and wires frequently use cable isolations with base of formulations of PVC, in those that stabilizer has usually been used with the help of heavy metals, as the lead, which is toxic. To solve the problem, from the 2002 one has come studying in combined form in the National Institute of Nuclear Research ININ and the Center of Investigation in Applied Chemistry CIQA, the modifications induced by the radiation in formulations with the help of vinyl poly chloride PVC. In these formulations, prepared with cross linking agent, plastifying industrial grade, stuff and non toxic stabilizers of calcium estearate and zinc industrial grade, it is sought to replace the stabilizer of Pb. For this were irradiated it test tubes of PVC with gamma radiation of cobalt 60 to three different dose in atmospheres of air and argon. Later it was determined their thermal stability at different times of heating and it was measured the Young modulus by means of thermo mechanical analysis. Those results obtained together with other techniques of characterization suggest that the irradiated proposed formulation can substitute the one stabilized with lead. (Author)

  3. Effect of Irradiation of 60CO Gamma Rays on Growth of Garlic (Allium Sativum L) Plants Cv. Lumbu Hijau at Low Land Area

    International Nuclear Information System (INIS)

    Garlic originally come from the sub tropical area. In Indonesia, garlic is grown generally in high land area with an altitude between 1000 - 1600 m above sea level. Therefore, the area for growing and producing garlic is limited. Besides, genetic variation of garlic is very narrow since garlic belongs to vegetatively propagated crops. An effort for increasing genetic variation of garlic was done by exposing garlic cloves to gamma rays in order to obtain garlic mutant lines adapted to low land area. Garlic cloves were exposed to different doses of gamma rays 0 (untreated) 2, 4, 6, 8 and 10 Gy at the Centre for Research and Development of Isotope and Radiation Technology. Each dose consisted of 150 garlic cloves. Untreated and irradiated garlic cloves were grown at Bandar Buat Experimental Station (50 m above sea level), Padang, West Sumatera. The experiment was arranged in a randomized block design with three replication. The parameter observed were percentage of grown plants and survival harvested plants, plant height, leaf number, chlorophyll content, number of stomata, plant age, number of cloves per bulbs, fresh, dry weight and diameter of bulbs. The result indicated that the dose of gamma rays 6 Gy is an advantage dose for obtaining well adapted garlic mutant lines in the low land area. (author)

  4. Humoral immune response of C57Bl/6j and BALB/c mice immunized with irradiated tachyzoites of Toxoplasma gondii RH strain and oral challenge with ME-49 strain

    Energy Technology Data Exchange (ETDEWEB)

    Jimenez Galisteo Junior, Andres [Instituto de Pesquisas Energeticas e Nucleares IPEN/CNEN-SP, Sao Paulo, SP (Brazil). Centro de Biotecnologia; Instituto de Medicina Tropical de Sao Paulo, Sao Paulo, SP (Brazil). Lab. de Protozoologia; E-mail: galisteo@usp.br; Zorgi, Nahiara Esteves; Andrade Junior, Heitor Franco de [Instituto de Medicina Tropical de Sao Paulo, Sao Paulo, SP (Brazil). Lab. de Protozoologia; Alves, Janaina Baptista; Nascimento, Nanci do [Instituto de Pesquisas Energeticas e Nucleares IPEN/CNEN-SP, Sao Paulo, SP (Brazil). Centro de Biotecnologia; Hiramoto, Roberto Mitsuyoshi [instituto Adolfo Lutz, Sao Paulo, SP (Brazil)

    2007-07-01

    Toxoplasmosis, a prevalent widespread infection in man and animals, is mainly transmitted by oral route, through ingestion of oocysts from water and food contaminated with cat feces or infected animal tissue cysts in undercooked meat. Vaccine development implies in effective intestinal immunity, the first site of parasite entry. Radiation (255 Gy/{sup 60}Co) sterilized T. gondii RH strain tachyzoites (RST) induced significant protection when parentally administered, similar to chronically infected and acute disease protected animal. We study the humoral immune response in C57Bl/6j and BALB/c mice immunized with 10{sup 7} RST, by oral (with aluminium hydroxide 3%) or parenteral 3 biweekly administrations. T. gondii antigens specific ELISA for IgG, IgA, IgG1, IgG2a and IgG2b detection was performed in weekly blood samples during immunization. Also we evaluate of the intestinal epithelial of immunized mice the integrity of the radiated parasites by electronic microscopy. After 2 weeks, immunized and control animals were challenged with 10 cysts of ME-49 strain p.o. Protection was determined at the 30th day by brain cyst counting. As it was possible to observe in the intestinal mucosal, the aluminium hydroxide seems to maintain unchanged the parasite morphology and its mechanisms of invasion, probably due to keeping it safe from extreme pH condition of stomach. All immunized groups presented significant protection when challenged with ME-49; however, BALB/c mice showed better protection levels, with only one positive animal on brain microscopic analysis. IgG production in the serum of the animals was higher in groups immunized by i.p route, however, IgA and IgG1 levels were higher in BALB/c mice immunized by oral route. This higher protection found in BALB/c group could probably also be related to the Th2 response, demonstrated by higher IgG1 levels. All these data provide insights in oral immunization schedules for toxoplasmosis prevention, suggesting that oral

  5. The Research on Effect of 60CoγIrradiation on Radiolysis Behavior of BOPA/CPP Packaging Materials by GC/MS%气相色谱质谱联用法分析60Coγ对包装材料双向拉伸尼龙/流延聚丙烯(BOPA/CPP)的辐解行为的影响

    Institute of Scientific and Technical Information of China (English)

    王宽强; 贺承启; 张勋龙; 彭秧

    2015-01-01

    This article researched radiolysis Behavior of 60 Coγirradiated BOPA/CPP Packaging Material by GC/MS. In full scan mode,all the 60 compounds were determined by spectrum,the mainly new radiolysis products were benzene,1,3 bis (1,1-dimethylethyl)and 2,5-Cyclohexadiene1,4-dione;At the same time,the contents of benzene,1,3,bis(1,1-di⁃methylethyl);Phenol,2,4-bis(1,1-dimethylethyl);2,5-Cyclohexadiene1,4-dione;Phenol 2,6--bis(1,1-dimethyleth⁃yl ) ,2,6-bis(1,1-dimethylethyl);Bis(cis-13-docosenamido)methane had obvious changes ,but the other radiolysis products had few changes at different irradiation intensities.%利用气相色谱质谱联用法研究了,受不同强度60Coγ辐照的包装材料BOPA/CPP的辐解行为;经过谱库检索,共检出60多种辐解产物,其中新检出主要的辐解产物有1,3-二叔丁基苯、2,6-二叔丁基苯醌;在不同的辐照强度下,1,3-二叔丁基苯,2,4二叔丁基苯酚,2,6二叔丁基对苯醌,4-乙基,2,6-二叔丁基苯酚,顺芥酸酰胺等物质发生了较明显的变化,其余辐解物没有发生太大的变化。

  6. Effects of 60Co administration on early placental cells

    International Nuclear Information System (INIS)

    The effects of 60Co administration on early placental cells were studied. Placental tissue and embryo obtained by induced abortion (6 - 13 weeks gestational age) were placed in the minimal essential medium (MEM) and irradiated with various doses of 60Co. After irradiation, the villi were cultured in a CO2 incubater at 370C. Cell growth process was observed every day with the phase-contrast microscope. Between 1 and 5 days epitheloid cells were dominant, but from about 7th day on fibroblastic cells dominated the culture. In placental tissue irradiated with 100, 200, 500 rad, fibroblastic cells began to grow earlier than in non-treated. Over 3000 rad 60Co inhibited the growth of cells and a culture was impossible. For each dose, the tissue was incubated for various periods of time, exposed to tritiated thymidine for the last hour and autoradiogram was prepared by the dipping method. The labeling index of irradiated trophoblasts showed a significant decrease compared with controls. A chromosome study was made in irradiated in vitro cell lines of fetus and placenta. There was no significant difference between the two cell lines concerning the frequency of chromosome aberration, which tended to increase as the chromosome becomes longer. It is concluded that the trophoblast is highly radiosensitive and that irradiation early in pregnancy may damage DNA synthesis in the trophoblast, and induce abortion. (author)

  7. Detection Method of TOXOPLASMA GONDII Tachyzoites

    Science.gov (United States)

    Eassa, Souzan; Bose, Chhanda; Alusta, Pierre; Tarasenko, Olga

    2011-06-01

    Tachyzoites are considered to be the most important stage of Toxoplasma gondii which causes toxoplasmosis. T. gondii is, an obligate intracellular parasite which infects a wide range of cells. The present study was designed to develop a method for an early detection of T. gondii tachyzoites. The method comprised of a binding assay which was analyzed using principal component and cluster analysis. Our data showed that glycoconjugates GC1, GC2, GC3 and GC10 exhibit a significantly higher binding affinity for T. gondii tachyzoites as compared to controls (T. gondii only, PAA only, GC 1, 2, 3, and 10 only).

  8. Study of the {sup 60}Co speciation in the aqueous radioactive waste of the la Hague nuclear reprocessing plant; environmental behaviour after discharges in the waters of the channel; Etude de la speciation du {sup 60}Co dans les effluents de l'usine de retraitement de combustibles irradies de la Hague; devenir apres rejet dans les eaux de la Manche

    Energy Technology Data Exchange (ETDEWEB)

    Gaudaire, J.M

    1999-07-01

    {sup 60}Co is produced as an activation product and is present in the low-level aqueous radioactive waste released from the La Hague plant. At present, the concentration in the sea (non filtered at 0.45 {mu}m) at the Goury site are close to or even below, the detection limit: 0.2 mBq.l{sup -1}. The {sup 60}Co speciation depends on the type of effluent considered: in the effluent A ('active'), the cobalt is in the form of a stable trivalent complex; in the effluent V (to be checked), the cobalt is in majority (50% of the activity release) in the form of particles (>0.45 {mu}m), and then in the form of two soluble species: ionic divalent (Co{sup 2+}) and some stable complexes. The evolution of the reprocessing techniques used does not affect the speciation. So, since the nuclear reprocessing plant started at the La Hague plant in 1966, the chemical species discharged in the sea shows time variation related to the evolution of the type of effluent discharged. Thus, since 1994, the particles of cobalt are the main species discharged in the Channel (the V effluents represent more than 85% of the total {sup 60}Co activity released). The effect of instantaneous dilution into the marine conditions involving a variation of pH, oxido-reduction, ionic strength, a gradient of salinity, does not interfere with the evolution of the chemical species discharged. Nevertheless, during the discharge of the V effluent, the main constituents of the sea water (Mg{sup 2+} and Ca{sup 2+}) go through a precipitation. This comes with the coprecipitation of the ion Co{sup 2+} and with the particles of cobalt (complexes are not affected), and it can be responsible for an increase in the concentration in the particles. The chemical behaviour of the cobalt in the Channel is different from those of conservative element such as antimony. The ionic cobalt and the particles have a small dispersion in the water (cobalt has a very high particle/dissolved distribution factor, it is a non

  9. 60Co levels in the seawater regions

    International Nuclear Information System (INIS)

    In order to assess the contribution from nuclear power facilities to the 60Co levels in seawater regions, it is essential to grasp the background values of 60Co. The following matters are described: 60Co sources and the respective inputs; nuclear test fallout and nuclear power plants; the 60Co levels in overseas countries; the 60Co levels in Japan; the 60Co levels from nuclear power plants in Fukui prefecture. In the seawater regions around Japan, there have been numerous instances of 60Co detection; several pCi/kg of dry earth in sea bottom earth and about 1 pCi/kg of raw material in marine life can be considered as the background levels due to nuclear test fallout and nuclear-powered submarines. In the seawater regions of Fukui prefecture, the 60Co levels appreciably exceeded the above background due to the nuclear power plants, which are insignificant concerning the radiation exposure of the local people. (Mori, K.)

  10. 60Co-γ射线辐射对美女樱·菠萝菊根长·芽长及出芽率的影响%Effect of the Treatment with 60Co-γ Ray Irradiation on the Germination Rate and the Length of Bud and Root of Verbena hortensis and Carthamus tinctorius

    Institute of Scientific and Technical Information of China (English)

    吴光升; 强继业; 陈立

    2005-01-01

    对美女樱和菠萝菊种子进行4种剂量的60Co-γ射线处理,结果显示:40Gy的辐射剂量可促进美女樱出芽率,而10、20、80Gy的辐射剂量抑制出芽率.辐射剂量和芽长、根长呈正相关.80Gy辐射剂量对美女樱的出芽率、芽长、根长较适宜.对菠萝菊而言,辐射剂量和出芽率、根长呈负相关.辐照对菠萝菊种子起抑制作用,说明菠萝菊不适合此范围的高剂量辐照.其中辐射剂量为20、80Gy时,菠萝菊种子不发芽;辐射剂量为40 Gy时,芽长达到了4.91 mm,远远大于对照0.57 mm.

  11. Effect of Different Polar Parts Extracted from Mucuna Birdwoodiana Tutcher on Peripheral Blood Counts of 60Coγray Irradiated Mice%白花油麻藤不同极性部位对60Coγ射线辐射小鼠外周血象的保护作用

    Institute of Scientific and Technical Information of China (English)

    王东晓; 陈若芸; 刘屏; 巩婷; 陈桂芸; 于腾飞; 马晓菲

    2008-01-01

    目的 观察白花油麻藤不同极性部位对骨髓抑制小鼠外周血象的影响,初步明确其促进造血的有效活性部位.方法 60 Coγ射线亚致死量辐射造成骨髓抑制小鼠模型,灌胃给予白花油麻藤各部位药液,于给药后1、3、7、14、21、28d检测其外周血白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、血小板(PLT).结果 辐射后小鼠外周血WBC、RBC、HGB、PLT较正常组明显下降,给予白花油麻藤各部位治疗后,其下降趋势减缓,与模型组比较,白花油麻藤95%部分和水部分可显著促进辐射小鼠外周血WBC、RBC、HGB、PLT的恢复(P<0.05).结论 白花油麻藤50%乙醇提取物的95%洗脱部分和水洗脱部分可明显促进辐射所致骨髓抑制小鼠外周血象的恢复,可能是白花油麻藤促进造血的有效活性部位.

  12. Effects of heterogenous suberect spatholobus stem on peripheral blood cell counts of 60Co γ ray irradiated mice%同名异种鸡血藤对60Co γ射线辐射小鼠外周血象的影响

    Institute of Scientific and Technical Information of China (English)

    王虹; 刘屏; 戴畅; 王东晓

    2007-01-01

    目的:研究密花豆藤(Spatholobus suberectu)、白花油麻藤(Mucuna birdwoodiana)、皱果崖豆藤(Millettia oosperma)、香花崖豆藤(Millettia dielsiana)等4种同名异种鸡血藤对骨髓抑制小鼠外周血象的作用.方法:60Co γ射线亚致死量辐照造成骨髓抑制小鼠模型,灌胃给药,于给药后1、3、7、14和21 d监测其外周血白细胞、红细胞和血小板计数.结果:给予同名异种鸡血藤治疗后,小鼠外周血白细胞、红细胞和血小板计数下降趋势减缓,与对照组比较,白花油麻藤和密花豆藤高、低剂量组的白细胞、红细胞和血小板计数升高,差异有统计学意义(P<0.05).结论:同名异种鸡血藤均可在一定程度上促进辐射小鼠外周血象的恢复,其中密花豆藤和白花油麻藤作用相对较好.

  13. Ion exchange-based treatment of 60Co contaminated well-water for storing γ irradiation source%离子交换法处理60Co污染的γ辐照装置源井水

    Institute of Scientific and Technical Information of China (English)

    毕梦; 缪世林; 张晓露; 张友九

    2014-01-01

    Objective To select an efficient ion exchange resin to purify the 60Co contaminated well-water for storing radioactive source and to ensure the radioactivity of 60Co in treated well-water below 10 Bq/L.Methods The radioactivity of 60Co in the water samples was measured by using the potassium cobaltinitrite coprecipitation-β counting method.The treatment efficiencies of two different ion exchange resins for the simulated 60Co-bearing waste water were compared to select a better one to dispose of the 60Co contaminated well-water.Results The treatment efficiency of MBD-15-SC mixed ion exchange resin was about 5.8 times higher than ZGCNR50 strong-acid cation exchange resin.The radioactivity of 60Co in the contaminated well-water could be reduced from 4.16 × 105 Bq/L to 1.16 Bq/L by two-stage sorption of MBD-15-SC mixed ion exchange resin.Conclusions Using several times of two-stage MBD-15-SC mixed ion exchange resin could effectively purify the 60Co contaminated well-water.The quality of the treated well-water could meet the sewage discharge standards.%目的 选择合适的离子交换树脂处理60 Co污染的贮源井水,使污染的贮源井水中放射性水平低于10 Bq/L,满足排放要求.方法 通过亚硝酸钴钾共沉淀-β计数法测量水样中60Co的活度,比较两种不同离子交换树脂处理60Co污染模拟井水的效果,并选择净化效果较好的离子交换树脂处理60Co污染的源井水.结果 MBD-15-SC型混合离子交换树脂对模拟井水中60Co净化效果明显高于ZGCNR50型强酸性阳离子交换树脂,大约为5.8倍;采用多次两级MBD-15-SC型混合离子交换树脂循环处理污染的源井水,可使水中60Co放射性浓度从4.16×105 Bq/L降至1.16 Bq/L.结论 采用多次两级MBD-15-SC型混合离子交换树脂可有效处理60 Co污染的源井水,处理后的贮源井水符合排放要求.

  14. Study of the effect of gamma irradiation ({sup 60} Co) on the quality of sugar cane spirit and on the cask of maturation; Estudo do efeito da irradiacao gamma ({sup 60} Co) na qualidade da cachaca e no tonel de envelhecimento

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, Mariana Branco de; Horii, Jorge; Alcarde, Andre Ricardo [Universidade de Sao Paulo (USP), Piracicaba, SP (Brazil). Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Dept.de Agroindustria, Alimentos e Nutricao]. E-mails: jhorii@esalq.usp.br; aralcard@esalq.usp.br

    2006-10-15

    The ageing or maturing process of drink improves the sensorial characteristics of the product making it a qualified drink with higher economical value. The traditional maturing method of drinks involves their interaction with wood and irradiation can accelerate this ageing process. Sugar cane spirit and oak barrels with a 20 L capacity were treated using gamma irradiation (150 Gy). Physical-chemical and chromatographic analyses were constantly performed for 390 days during the drink ageing period. The sugar cane spirit and barrel irradiation did not change most volatile components of the same type coefficient such as volatile acidity, esters, superior alcohols and furfural throughout the 390 days. There is evidence, however, that some component parameters like aldehydes, tannin, color and copper concentration are in some way influenced resulting in partial acceleration of the ageing or maturing process. At the end of the ageing period, a sensorial analysis was made using 30 non-trained people who tasted the drink. The ageing process acceleration was confirmed by the sensorial evaluation, where the irradiated sugar cane spirit and/or barrel had higher approval regarding all parameters analyzed (scent, flavor and appearance). (author)

  15. Sorption of 60 Co on inorganic solids

    International Nuclear Information System (INIS)

    The behavior of sorption of the 60 Co in aqueous solution under static conditions to different values of pH of the aqueous solution (1, 3, 5, 7, and 10) on MgO, MnO2, SnO, TiO2, activated carbon and calcinate hydrotalcite was investigated. It was found that the best sorbents of the 60 Co was the MnO2, activated carbon and TiO2 whose sorption was incremented when increasing the pH value of the aqueous solutions, in the one case of the hydrated oxides, the 60 Co interacted with the electrically charged surface of the sorbents that depends on the pH of the solution and of the point of zero charge (zpc) of the sorbent. (Author)

  16. Estudo do efeito da irradiação gamma (60CO na qualidade da cachaça e no tonel de envelhecimento Study of the effect of gamma irradiation (60co on the quality of sugar cane spirit and on the cask of maturation

    Directory of Open Access Journals (Sweden)

    Mariana Branco de Miranda

    2006-12-01

    Full Text Available O processo de envelhecimento ou maturação das bebidas proporciona uma melhora nas características sensoriais da cachaça, tornando-a de qualidade superior e de maior valor econômico. O método tradicional de maturação de bebidas é sua interação com madeiras, sendo que a irradiação pode acelerar este processo de envelhecimento. A cachaça e os tonéis de carvalho de 20 L de capacidade foram submetidos à irradiação gama (150 Gy. Análises físico-químicas e cromatográficas foram realizadas periodicamente ao longo de 390 dias do período de envelhecimento da bebida. A irradiação da cachaça e do tonel não alterou a maioria dos componentes voláteis do coeficiente de congêneres como acidez volátil, ésteres, álcoois superiores e furfural durante os 390 dias. Há evidências, entretanto, de que os parâmetros de alguns componentes como aldeídos, taninos, cor e teor de cobre são de alguma forma influenciados, resultando em aceleração parcial do processo de maturação ou envelhecimento. Ao final do período de envelhecimento, foi feita uma análise sensorial com 30 provadores não treinados. A aceleração do processo de envelhecimento foi confirmada pela avaliação sensorial, e a cachaça e/ou tonel irradiados receberam maior indicação de aprovação em todos os parâmetros analisados (aroma, sabor e aparência.The ageing or maturing process of drink improves the sensorial characteristics of the product making it a qualified drink with higher economical value. The traditional maturing method of drinks involves their interaction with wood and irradiation can accelerate this ageing process. Sugar cane spirit and oak barrels with a 20 L capacity were treated using gamma irradiation (150 Gy. Physical-chemical and chromatographic analyses were constantly performed for 390 days during the drink ageing period. The sugar cane spirit and barrel irradiation did not change most volatile components of the same type coefficient such

  17. Gamma 60Co DL50/30 of Biomphalaria glabrata (Say, 1818) DL50/30 raios gama de 60Co em Biomphalaria glabrata (Say, 1818)

    OpenAIRE

    Elaine Barros da Costa CARVALHO; Melo, Ana Maria Mendonça de Albuquerque; Mauricy Alves da MOTTA

    1999-01-01

    The variation of resistance to 60Co gamma-rays of Biomphalaria glabrata was studied. A population of 480 mollusks was observed during 30 days - distributed in 8 groups of snails isolated and 8 groups of snails in colonies - after exposure (30 snails per group per dose) to increasing doses of gamma radiation. Doses of 10, 20, 40, 60, 80, 160, 320 and 640 Gy from a Gamma-cell 60Co irradiator, were applied to the test groups and two groups control (non-irradiated) of snails - isolated and colony...

  18. Microstructural study of Y{sub 1}Ba{sub 2}Cu{sub 3}O{sub 7-x}/Ag samples irradiated with {sup 60}Co {gamma} rays at high doses

    Energy Technology Data Exchange (ETDEWEB)

    Rangel, R. [Programa de Postgrado en Fisica de Materiales, Centro de Investigacion Cientifica y de Educacion Superior de Ensenada, Apartado Postal 2681, CP 22800, Ensenada, BC (Mexico); Galvan, D.H. [CECIMAC-UNAM, Apartado Postal 2681, CP 22800, Ensenada, BC (Mexico); Adem, E. [Instituto de Fisica-UNAM, Apartado Postal 20-364, CP 01000, Mexico DF (Mexico); Bartolo-Perez, P. [CINVESTAV-IPN Unidad Merida, Departamento de Fisica Aplicada, Merida, Yucatan (Mexico); Maple, M.B. [Physics Department and Institute for Pure and Applied Physical Sciences, La Jolla, CA (United States)

    1998-06-01

    We have investigated the damage induced by irradiation in Y{sub 1}Ba{sub 2}Cu{sub 3}O{sub 7-x} silver added samples. The samples were prepared with 0 and 6.5 wt% of silver and irradiated by high-energy {gamma} irradiation (50-150 Mrad). The roles of silver and dosage irradiation are discussed in terms of their effects on microstructure, crystallinity, critical temperature (T{sub c}) and zero-resistance temperature (T{sub 0}). After irradiation, T{sub c} decreased while the room-temperature electrical resistance increased by a factor of 8 for some of the samples. The difference in T{sub 0} between irradiated and non-irradiated YBCO samples was of the order of 10 K. We have found that the difference is bigger for silver-added samples. We have also observed several changes in diffraction patterns of YBCO and YBCO-silver samples. SEM images, EDS and XPS analysis showed that silver resided inside the grains as single atoms and as metallic clusters. The relative concentrations of the elements in samples were quantified by Auger electron spectroscopy. The values showed a gradual increase for radiation doses ranging between 0 and 100 Mrad. For doses up to 100 Mrad, J{sub c} decreased because of the weak-link breakage induced by high doses of {gamma} rays. (author)

  19. Initial research of screening for the differentially expressed proteins in beagles irradiated with 4.5 Gy 60Co γ-rays by two-dimensional gel electrophoresis and mass spectrometry

    International Nuclear Information System (INIS)

    Objective: To explore the mechanisms of cytokines on acute radiation disease in irradiation beagles. Methods: The sera of beagles irradiated with 4.5 Gy γ-rays with cytokines treatment was collected at different time points post irradiation. The two-dimensional gel electrophoresis (2-DE) was differentially expressed proteins with significance, and the amino acid sequences should be determined. Results: High resolution 2-DE gel map was obtained. There were six differentially expressed proteins in sera of irradiated beagles at different time points. Four protein spots were successfully identified by MS. A significant spot was identified as serum amyloid A (SAA) by HD-MS, with relative molecular mass of 13 077 and isoelectric point of 6.26. Expression of SAA was not found 1 d pre-irradiation and 36 d post-irradiation, but increased slightly 1 d (0.2166) and significantly 14 d post-irradiation (0.4577). Conclusions: The expression of serum amyloid A was consistent with the process of acute radiation injury, which might indicate the turnover of the disease. (authors)

  20. EFFECT OF 60COγ-RAYS IRRADIATION ON THE PROTEIN CONTENTS IN Deinococcus radiodurans%60Coγ射线辐照对耐辐射菌Deinococcus radiodurans 蛋白质含量的影响

    Institute of Scientific and Technical Information of China (English)

    孙翠凤; 刘芬菊; 孔向蓉; 陈剑; 宁萍

    2003-01-01

    研究了耐辐射菌对60Coγ射线的辐射抗性,观察不同剂量照射后,细菌中蛋白质含量、辐照剂量及照射后培养不同时间的关系.应用平板菌落计数法,计数不同剂量辐照后的克隆数,计算存活率,绘制剂量-存活曲线.采用紫外分光光度法检测细菌中蛋白质的含量.结果表明,耐辐射菌的存活曲线呈肩型,具有极强的辐射抗性.蛋白质的含量随着照射剂量的升高而不断增加,当辐射剂量达到5kGy时,蛋白质含量最高(p<0.01);若受照剂量>5kGy时,则蛋白质含量随受照射剂量的升高而逐渐降低.5kGy辐照后,随照射后温育时间的延长,蛋白质含量不断降低,培养时间为6h,蛋白质的含量最低(p<0.01),与对照组(未受照射组)相比,无显著性差异(p>0.05).

  1. Sorption of {sup 60} Co on inorganic solids; Sorcion de {sup 60} Co en solidos inorganicos

    Energy Technology Data Exchange (ETDEWEB)

    Granados C, F.; Bulbulian G, S. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico); Mardel V, B. [UAM-I, 09340 Mexico D.F. (Mexico)

    2003-07-01

    The behavior of sorption of the {sup 60} Co in aqueous solution under static conditions to different values of pH of the aqueous solution (1, 3, 5, 7, and 10) on MgO, MnO{sub 2}, SnO, TiO{sub 2}, activated carbon and calcinate hydrotalcite was investigated. It was found that the best sorbents of the {sup 60} Co was the MnO{sub 2}, activated carbon and TiO{sub 2} whose sorption was incremented when increasing the pH value of the aqueous solutions, in the one case of the hydrated oxides, the {sup 60} Co interacted with the electrically charged surface of the sorbents that depends on the pH of the solution and of the point of zero charge (zpc) of the sorbent. (Author)

  2. Different gamma ray (60 Co) dose effects on Sorghum genotype germination

    International Nuclear Information System (INIS)

    In agriculture, applying irradiation is a very valuable way of obtaining vegetable products for human and animal consumption. Cobalt-60, one of the main sources of gamma-rays, is considered an important tool in plant breeding programs, which have the objective of promoting genetic variability of cultivars with resistance to adverse environments. In this research, the effects of different 60Co doses on germination vigor and seed germination velocity of forage sorghum genotypes were evaluated. The study was carried out at the IPA (Empresa Pernambucana de Pesquisa Agropecuaria) in Recife, Pernambuco, Brazil. The work was installed in germination boxes, following laboratory recommendations. Thus, a experiment involving three sorghum genotypes (IPA 467-4-2, IPA 02-03-01, and Sudan 4202), five 60Co doses (Zero, 150, 300, 350, and 400 Gy), was set up. The sees were irradiated before the beginning of the experiment being exposed to gamma rays from a 60Co-source (cobalt irradiator) at DEN (Nuclear Engineering Department) of the UFPE (Pernambuco Federal University), Brazil. The work also had the objective of evaluating the sorghum genotypes x 60Co dose interaction. The main results obtained showed that the sorghum genotype IPA 02-03-01 presented the greatest values of germination and vigor percentages, and seed germination velocity, when compared to the others evaluated, on 350 and 400 Gy of 60Co doses. (author)

  3. Tachyzoites of Toxoplasma gondii irradiated with 255 Gy induces decrease of cysts and cerebral lesions in mice challenged with cysts of ME-49; Taquizoitos de Toxoplasma gondii irradiados com 255 Gy induzem diminuicao de cistos e lesoes cerebrais em camundongos desafiados com cistos da cepa ME-49

    Energy Technology Data Exchange (ETDEWEB)

    Hiramoto, Roberto Mitsuyoshi; Galisteo Juniorm Andres Jimenez; Nascimento, Nanci do; Andrade Junior, Heitor Franco de [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil). Lab. de Biologia Molecular]. E-mail: rmhiramoto@bol.com.br; hfandrad@usp.br

    2002-07-01

    Toxoplasmosis can cause ocular lesions in normal individuals and several diseases in foetus, HIV infection and transplants. Toxoplasma gondii has a complex life cycle, involving cats, as the definitive host, and warm blood species, as intermediated hosts. The infection occurs by ingestion of food and water contaminated with infected cat faeces, contaminated milk and cheese or raw and undercook meat of the intermediated hosts. To date, there is no commercial vaccine of use in humans. In this work, tachyzoites of T. gondii RH strain were irradiated with 255 Gy and inoculated in C57Bl/6j mice (3 doses, biweekly), after mice were challenged with 1, 5, 10, 20 and 25 cysts of ME-49 by oral gavage. The lesions and cysts in the brain were analyzed in all mice, after 4-week post infection. The mortality was 20% in control mice (ME-49 cysts only) and not one in immunized mice. The number of cysts was high in the control group, but low in immunized 255 Gy mice (n<100). Immunized mice showed less cerebral pathology and necrosis foci. Ionizing radiation is an important tool in the study toxoplasmosis and vaccine development. (author)

  4. THE RESTORATIVE EFFECT OF MOUSE INTESTINAL RNA ON THE SMALL INTESTINE OF MICE OF THE SAME STRAIN AFTER γ RAY IRRADIATION%小鼠小肠RNA对受60Co γ射线照射同系小鼠小肠损伤的恢复

    Institute of Scientific and Technical Information of China (English)

    曾桂英; 田芙蓉; 陈永斌; 任东青; 崔大祥

    2001-01-01

    用不同剂量的60Co γ射线照射BALB/c小鼠的全身或腹部,于照射后1-3h内注入同系正常小鼠小肠RNA,通过肠腺存活率的测定,观察小肠RNA显效时间和照射方式对其修复作用的影响。结果表明,(1)小鼠受照射后6h空肠肠腺存活率即开始降低,4d时降至最低值;(2)受腹部照射小鼠在小肠RNA注入后6h肠腺存活率比照射对照组提高21.40%;(3)正常小鼠小肠RNA在促进受全身照射小鼠十二指肠、空肠和回肠恢复时的DMF(Dose Modifying Factor)分别为1.17、1.12、1.10。结果说明,小肠RNA不仅可提高受腹部照射同系小鼠空肠肠腺存活率,而且也可提高受全身照射同系小鼠十二指肠、空肠和回肠的肠腺存活率,并在注入后6h即可表现出来。%Mouse intestinal RNA was injected into the mice of the same strain within 1-3h after different doses of abdominal or whole body 60Co γ irradiation, so as to explore the initial effective time of mouse intestinal RNA and the affection of radiation condition on its restorative effect, by measuring the survival of mouse intestinal crypt. The results showed (1) A decrease in the survival of mouse intestinal crypt began 6h after the irradiation, and the lowest survival rate appeared on the fourth day. (2) The survival of mouse intestinal crypt of the abdominal irradiated mice increased 21.4% at 6h after intestinal RNA injection as compared with that of the irradiated control group. (3) The dose modifying factor (DMF) of normal mouse intestinal RNA in the promotion of the recovery of the duodenum, jejunum and ileum of mice after whde body irradiation 1.17, 1.12 and 1.10 respectively. The above results suggest that mouse intestinal RNA can raise not only the survival of jejunum crypt of the mice of the same strain after abdominal irradiation but also the survival of crypt of the duodenum, jejunum and ileum of the mouse after whole body irradiation, which may be

  5. Effect of 60CO radiation processing in mate (Ilex paraguariensis)

    International Nuclear Information System (INIS)

    The mate (Ilex paraguariensis), a native species from South America, is mainly consumed as typical beverage called chimarrao and terere. An important problem that has been afflicting this product since a long time is its natural fungal contamination responsible to affect its physical, health and nutritional qualities. In order to improve this product quality, radiation processing can be effective in reducing pathogens levels, with minimal nutritional and sensory changes. The aim of this study was to evaluate the effectiveness of gamma radiation from 60Co at doses 0, 3, 5, 7 and 10kGy in reducing fungal contamination in mate, as well as analyze its nutritional and sensory characteristics. The following methodologies were applied: analysis of yeast and mold, total phenolic compounds analysis, antioxidant analysis, quantification of phenolic compounds and xanthines by liquid chromatography and sensory analysis. Microbiological analysis showed a decreasing molds and yeasts growth with increasing radiation doses. Regardless of the radiation dose applied there were no decrease of total phenolic compounds in both infusions. Chimarrao samples irradiated with 7 and 10kGy showed a decrease in the DPPH radical-scavenger activity, nevertheless for terere samples, there were no significant difference. Chimarrao chromatographic profile did not show a variation on xanthines quantification, however a 10kGy radiation dose caused a change to phenolic compounds quantitative profile. Terere samples did not show any significant difference to any analyzed compounds. Sensory analysis did not exhibit a significant difference between irradiated and non irradiated chimarrao samples, as well as between irradiated and non irradiated terere samples. It could be concluded that gamma radiation processing of mate may be a feasible alternative to industry, since there was a reduction on fungal contamination, without changes in sensory qualities and with minimum alterations in quantitative and

  6. Effect of 60Co radiation processing in mate (Ilex paraguariensis)

    International Nuclear Information System (INIS)

    The mate (Ilex paraguariensis), a native species from South America, is mainly consumed as typical beverage called chimarrao and terere. An important problem that has been afflicting this product since a long time is its natural fungal contamination responsible to affect its physical, health and nutritional qualities. In order to improve this product quality, radiation processing can be effective in reducing pathogens levels, with minimal nutritional and sensory changes. The aim of this study was to evaluate the effectiveness of gamma radiation from 60Co at doses 0, 3, 5, 7 and 10 kGy in reducing fungal contamination in mate, as well as analyze its nutritional and sensory characteristics. The following methodologies were applied: analysis of yeast and mold, total phenolic compounds analysis, antioxidant analysis, quantification of phenolic compounds and xanthines by liquid chromatography and sensory analysis. Microbiological analysis showed a decreasing molds and yeasts growth with increasing radiation doses. Regardless of the radiation dose applied there were no decrease of total phenolic compounds in both infusions. Chimarrao samples irradiated with 7 and 10 kGy showed a decrease in the DPPH radical-scavenger activity, nevertheless for terere samples, there were no significant difference. Chimarrao chromatographic profile did not show a variation on xanthines quantification, however a 10 kGy radiation dose caused a change to phenolic compounds quantitative profile. Terere samples did not show any significant difference to any analyzed compounds. Sensory analysis did not exhibit a significant difference between irradiated and non irradiated chimarrao samples, as well as between irradiated and non irradiated terere samples. It could be concluded that gamma radiation processing of mate may be a feasible alternative to industry, since there was a reduction on fungal contamination, without changes in sensory qualities and with minimum alterations in quantitative and

  7. Manufacture of Single ~(60)Co Source Irradiation Facility

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Reference radiation filed produced by isotope source is necessary for calibration of radiation dose meter. According to the GB/T 12162.1-2002 "X and gamma reference radiation for calibrating dosemeters and doserate meters and for determining

  8. Transfer of 60Co from midwater squid to sperm whales

    International Nuclear Information System (INIS)

    Sperm whales are notable squid-eaters. They feed mainly on medium to large-sized cephalopods at midwater levels and defecate near the surface. This suggests the existence of an upward transport of 60Co by sperm whales from the mesopelagic zone (150-1,200m). To elucidate this squid-whale route for this artificial radionuclide, 60Co content was determined in squid and in predator whales captured by commercial whaling. In the Cephalopoda livers 60Co levels of 30-500 mBq kg-1 wet were found and in the viscera of Odontoceti (toothed whales) 15-40 mBq kg-1 wet. About 0.3% of 60Co ingested was estimated to be retained in a 23-year-old male sperm whale. In the livers of Bryde's whales, 60Co levels of 40-80 mBq kg-1 wet were detected, but not in euphausiids and sardines, their possible prey. The level of Co in sperm whales was nearly the same as in Bryde's whales. Specific radioactivity 60Co/59Co in mBq μg-1 was several times higher in sperm whale (1.1-1.6) than in cephalopods (0.19-0.77). Eating prey with a high content of 60Co in the 1960's may have contributed to the present body burden in sperm whales with a long-life span. However, the origin of 60Co in Bryde's whales is unknown. (author)

  9. Evaluation of the radio modifier effect of propolis on chinese hamster ovary (CHO-K1) and human prostate cancer (PC3) cells, irradiated with 60-CO; Avaliacao do efeito radiomodificador da propolis em celulas de ovario de hamster chines (CHO-K1) e em celulas tumorais de prostata (PC3), irradiadas com CO-60

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Geyza Spigoti

    2011-07-01

    In the last decades, it has been given a great interest to investigations concerning natural, effective, nontoxic compounds with radioprotective potential together with the increasing utilization of different types of ionizing radiation for various applications. Among them propolis, a resinous compound produced by honeybees (Apis mellifera), has been considered quite promising, since it presents several advantageous biological characteristics, i. e., anti-inflammatory, antimicrobial, anticarcinogenic, antioxidant and also free radical scavenging action. The purpose of the present study was to evaluate the effect of Brazilian propolis, collected in the State of Rio Grande do Sul, on Chinese hamster ovary (CHO-K1) and human prostate cancer (PC3) cells, irradiated with {sup 60}Co {gamma} radiation. For this purpose, three interlinked parameters were analyzed: micronucleus induction, cell viability and clonogenic death. The choice of these parameters was justified by their biological significance, in addition to the fact that they are readily observable and measurable in irradiated cells. The cytogenetic data obtained showed a radioprotective effect of propolis (5-100 {mu}g/ml) in the induction of DNA damage for both cell lines, irradiated with doses of 1 - 4 Gy. The cytotoxicity assay, however, showed a prominent antiproliferative effect of propolis (50 - 400{mu}/ml) in PC3 cells irradiated with 5 G{gamma}. The survival curves obtained were adequately fitted by a linear-quadratic model, where the {alpha} coefficient was higher in CHO-K1 cells. Concerning the clonogenic capacity, PC3 cells were more radiosensitive than CHO-K1 cells at the higher doses of the survival curve. Propolis at the concentrations of 30 - 100 {mu}g/ml, did not influence the clonogenic potential of PC3 cells, since the survival curves, associated or not with propolis, were found similar, although the combined treatment in CHO-K1 cells exhibited a stimulating proliferative effect. The data

  10. 60Co照射后豚鼠内耳前庭终器毛细胞损伤及凋亡的实验研究%INJURY AND APOPTOSIS IN VESTIBULAR APPARATUS OF INNER EAR IN GUINEA PIGS AFTER 60Co IRRADIATION

    Institute of Scientific and Technical Information of China (English)

    孙贝蒂; 唐安洲; 谢利红

    2012-01-01

    目的:探讨60Co照射后豚鼠内耳前庭终器损伤与caspase-3、bax及bcl-2表达的关系及照射后前庭功能的损伤情况.方法:将白化豚鼠40只随机分为正常对照组和照射组(1,4,14,30 d组),每组8只.照射组豚鼠右耳颞部做一次性60Coγ射线照射40 Gy.分别于照射前、照射后1,4,14 d及30 d行冰水试验后处死,取出听泡,行前庭终器切片、HE染色、免疫组化染色,并分离囊斑及壶腹嵴,行超薄切片透射电镜检查.结果:冰水试验照射组眼震时间较对照组缩短,差异有统计学意义(P<0.05),照射后30 d组眼震时间较其他照射组长,差异有统计学意义(P<0.05).照射组豚鼠的囊斑较对照组感觉上皮层厚度变薄,毛细胞数量减少,纤毛变短、减少,照射组壶腹嵴耳石层消失,纤毛消失.透射电镜显示,照射后各组的囊斑及壶腹嵴均可见受损的毛细胞,受损毛细胞线粒体肿胀,以30 d为明显,线粒体嵴变短减少并且发生断裂.照射后各组囊斑caspase-3、bax、bcl-2表达较对照组增强,差异有统计学意义(P<0.05).照射后30 d组囊斑、壶腹嵴caspase-3表达强度较其他对照组减弱,差异有统计学意义(P<0.05).照射后4 d组囊斑、壶腹嵴bax表达上调程度较bcl-2表达上调程度强,差异有统计学意义(P<0.05),bax/bcl-2上升.照射后30 d组囊斑、壶腹嵴bcl-2的上调程度较bax表达上调程度强,差异有统计学意义(P<0.05),bax/bcl-2下降.结论:凋亡相关蛋白caspase-3的表达上调及bax、bcl-2的不均衡表达上调可能在60Coγ射线造成的前庭损伤中起重要作用.%Objective:To explore the damage of vestibular inner ear after Co irradiation and the relationship of vestibular apparatus injury and caspase-3, bax and bcl-2. Methods: Forty guinea pigs were randomly divided into 5 groups, including control (N) and radiation groups (Dl , D4, DM and D30). The right ear of the guinea pigs was radiated. The guinea pigs were sacrificed on

  11. Dosimetric response of united, commercially available CTA foils for 60Co gamma rays

    International Nuclear Information System (INIS)

    The usefulness of two kinds of untinted CTA foils: Fuji CTR-125 dosimetric foil and technical CTA-T foil, produced by 'Zaklady Chemiczne, 'Gorzow Wielkopolski' as support for light-sensitive layers of amateur photo-films, for 60Co gamma ray dosimetry was investigated. In spite of rather bad physical parameters of the technical foil (spread of foil thickness, high and different initial absorbance) the dosimetric response of both foils for 60Co gamma rays was similar. The CTA-T foil can be used for routine dosimetry providing that dosimetric signals have to be calculated exactly as recommended by the ASTM (American Society for Testing and Materials) standard, i.e. as the difference of absorbance of irradiated and (the same) non-irradiated foil. Any other approach may lead to high errors of dose evaluation. The last is true also for other CTA foils, especially after long self-life. (author)

  12. A positron 1D-ACAR spectrometer for the study of 60Co containing materials

    International Nuclear Information System (INIS)

    In order to study some micro-structural changes in irradiated nuclear reactor-pressure vessel steels using a positron annihilation technique, a new three-detector set-up, suitable for a positron 1-dimensional angular correlation of annihilation radiation (1D-ACAR) study of 60Co-containing materials, was developed. The design of the equipment as well as results from test measurements are described. (orig.)

  13. Decoloration Kinetics of Waste Cooking Oil by 60Co γ-ray/H2O2

    Science.gov (United States)

    Xiang, Yulin; Xiang, Yuxiu; Wang, Lipeng

    2016-03-01

    In order to decolorize, waste cooking oil, a dark red close to black solution from homes and restaurants, was subjected to 60Co γ-ray/H2O2 treatment. By virtue of UV/Vis spectrophotometric method, the influence of Gamma irradiation to decoloration kinetics and rate constants of the waste cooking oil in the presence of H2O2 was researched. In addition, the influence of different factors such as H2O2 concentration and irradiation dose on the decoloration rate of waste cooking oil was investigated. Results indicated that the decoloration kinetics of waste cooking oil conformed to the first-order reaction. The decoloration rate increased with the increase of irradiation dose and H2O2 concentration. Saponification analysis and sensory evaluation showed that the sample by 60Co γ-ray/H2O2 treatment presented better saponification performance and sensory score. Furthermore, according to cost estimate, the cost of the 60Co γ-ray/H2O2 was lower and more feasible than the H2O2 alone for decoloration of waste cooking oil.

  14. Study on the possibility of using a 60 Co therapeutical unity in Intensity Modulated Radiotherapy (IMRT)

    International Nuclear Information System (INIS)

    With the increasing advances in complex treatment techniques, there is a tendency to obtain more sophisticated equipment to deliver the dose. The use of 3D conformal radiotherapy is now routine in many radiotherapy facilities as well as the utilization of intensity modulated radiotherapy (IMRT). Both are usually implemented using linear accelerators equipped with multi leaves collimators, which create the conformity and the fluence distributions required. However, the complexity of increasingly sophisticated equipment, such as linear accelerators, requires a frequent quality control of their operation, as well as a detailed and constant maintenance. Even carrying out these procedures, the accelerators may present technical problems interrupting for a long time a treatment using the IMRT technique. Despite the clear practical and technological advantages that linear accelerators have on 60Co irradiators, these devices occupy an important place in radiotherapy, mainly due to the low cost of equipment installation and maintenance when compared to those required by accelerators. Many radiotherapy facilities that work with IMRT have tele therapeutic isocentric 60Co units. In principle, such equipment would be able to be used for treatment with IMRT using compensating blocks to modulate the beam. This study investigates this possibility and shows that it is feasible. The comparison of treatment plans of a head-and-neck cancer and other of a cancer of the central nervous system, based on a 60Co irradiator and a Linac 2300 C/D, presented advantages for the 60Co irradiator. Furthermore; the delivery of dose obtained with the two systems showed themselves equivalent when compared to their respective plans. (author)

  15. A comparison of ionizing radiation damage in CMOS devices from 60Co gamma rays, electrons and protons

    Institute of Scientific and Technical Information of China (English)

    HE Sao-Ping; YAO Zhi-Bin; ZHANG Feng-Qi

    2009-01-01

    Radiation hardened CC4007RH and non-radiation hardened CC4011 devices were irradiated using 80Co gamma rays, 1 MeV electrons and 1--9 MeV protons to compare the ionizing radiation damage of the gamma rays with the charged particles. For all devices examined, with experimental uncertainty, the radiation induced threshold voltage shifts (△Vth) generated by 60Co gamma rays are equal to that of 1 MeV electron and 1-7 MeV proton radiation under 0 gate bias condition. Under 5 V gate bias condition, the distinction of threshold voltage shifts (△Vth) generated by 60Co gamma rays and 1 MeV electrons irradiation are not large, and the radiation damage for protons below 9 MeV is always less than that of 60Co gamma rays. The lower energy the proton has, the less serious the radiation damage becomes.

  16. Susceptibility of various cell lines to Neospora caninum tachyzoites cultivation

    Directory of Open Access Journals (Sweden)

    Khordadmehr, M.,

    2014-05-01

    Full Text Available Neospora caninum is a coccidian protozoan parasite which is a major cause of bovine abortions and neonatal mortality in cattle, sheep, goat and horse. Occasionally, cultured cells are used for isolation and multiplication of the agent in vitro with several purposes. In this study the tachyzoite yields of N. caninum were compared in various cell cultures as the host cell lines. Among the cell cultures tested, two presented good susceptibility to the agent: cell lines Vero and MA-104. SW742 and TLI (in vitro suspension culture of lymphoid cells infected with Theileria lestoquardi showed moderate sensitivity. No viable tachyzoite were detected in the culture of MDCK and McCoy cell lines. These results demonstrate that MA-104 and SW742 cells present adequate susceptibility to N. caninum compared to Vero cells, which have been largely used to multiply the parasite in vitro. Moreover, these have easy manipulation, fast multiplication and relatively low nutritional requirements. In addition, the result of this study showed that TLI cell line as a suspension cell culture is susceptible to Nc-1 tachyzoites infection and could be used as an alternative host cell line for tachyzoites culture in vitro studies.

  17. Doses in sensitive organs during prostate treatment with a 60Co unit

    International Nuclear Information System (INIS)

    Using thermoluminiscent dosimeters the absorbed dose in the bladder, rectum and thyroid have been evaluated when 200 cGy was applied to the prostate. The treatment was applied with a 60Co unit. A water phantom was built and thermoluminiscent dosimeters were located in the position where the prostate, bladder, rectum and thyroid are located. The therapeutic beam was applied in 4 irradiations at 0, 90, 180 and 270° with the prostate at the isocenter. The TLDs readouts were used to evaluate the absorbed dose in each organ. The absorbed doses were used to estimate the effective doses and the probability of developing secondary malignacies in thyroid, rectum and bladder. - Highlights: • The absorbed doses in the bladder, rectum and thyroid were measured. • Measurements were done during prostate treatment with a 60Co unit. • TLD100s in a water phantom were used. • The effective doses were also estimated

  18. Evaluation of the Timepix chip radiation hardness using a {sup 60}Co source

    Energy Technology Data Exchange (ETDEWEB)

    Čarná, Mária; Dučevová, Kateřina; Hejtmánek, Martin, E-mail: hejtmark@fzu.cz; Konček, Ondřej; Marčišovský, Michal

    2013-12-21

    Radiation damage is a widely studied topic for its effects on detectors and supporting electronics in various practical applications. Radiation hardness and stability of the detector properties are critical parameters in applications of semiconductor radiation detectors. The 0.25μm CMOS technology used in fabrication of the Medipix2 and Timepix chips provides high degree of inherent radiation hardness. We present the study of operational, detection and signal processing properties of the irradiated Timepix chip exposed to a high-flux {sup 60}Co source reaching the operational limits of the chip. -- Highlights: • Radiation hardness of pixel detector based on Medipix2 against {sup 60}Co has been performed. • The {sup Co} was used as a radiation source due to its usage in number of practical applications. • Systematic study of γ radiation of various energies on the detector systems is desired.

  19. A comparison of pdd for 60Co teletherapy units

    International Nuclear Information System (INIS)

    Although BJR supplement No 17 provides TAR and PDD tables for 60Co gamma rays, due to inherent differences hat exists among individual teletherapy machine and their source it is essential to measure TAR and PDD that are applicable for user's teletherapy unit. TAR and PDD values for different field sizes at 80 cm SSD, open field, gantry angle 0o, collimator angle 0o were determined in CIRUS 60Co teletherapy unit by ionization chamber NE2570/1B Farmer type. PDD for a standard field size 10 10 cm2, open field, gantry angle 0o,Collimate angle 0o were determined in MEDNIF FYC 2600H 60Co teletherapy unit by using ionization chamber RT101. That experimentally measured PDD values for both units were compared with published values in BJR supplement No 17. The experimentally measured values for CIRUS 60Co showed up to 0.8% deviation at maximum and that for MEDNIF 60Co showed up to 4.5% deviation at maximum. This study investigated the discrepancy between the BJR values and experimentally determined values for two teletherapy units in Nepal

  20. Comparative analysis of 60Co intensity-modulated radiation therapy

    Science.gov (United States)

    Fox, Christopher; Romeijn, H. Edwin; Lynch, Bart; Men, Chunhua; Aleman, Dionne M.; Dempsey, James F.

    2008-06-01

    In this study, we perform a scientific comparative analysis of using 60Co beams in intensity-modulated radiation therapy (IMRT). In particular, we evaluate the treatment plan quality obtained with (i) 6 MV, 18 MV and 60Co IMRT; (ii) different numbers of static multileaf collimator (MLC) delivered 60Co beams and (iii) a helical tomotherapy 60Co beam geometry. We employ a convex fluence map optimization (FMO) model, which allows for the comparison of plan quality between different beam energies and configurations for a given case. A total of 25 clinical patient cases that each contain volumetric CT studies, primary and secondary delineated targets, and contoured structures were studied: 5 head-and-neck (H&N), 5 prostate, 5 central nervous system (CNS), 5 breast and 5 lung cases. The DICOM plan data were anonymized and exported to the University of Florida optimized radiation therapy (UFORT) treatment planning system. The FMO problem was solved for each case for 5-71 equidistant beams as well as a helical geometry for H&N, prostate, CNS and lung cases, and for 3-7 equidistant beams in the upper hemisphere for breast cases, all with 6 MV, 18 MV and 60Co dose models. In all cases, 95% of the target volumes received at least the prescribed dose with clinical sparing criteria for critical organs being met for all structures that were not wholly or partially contained within the target volume. Improvements in critical organ sparing were found with an increasing number of equidistant 60Co beams, yet were marginal above 9 beams for H&N, prostate, CNS and lung. Breast cases produced similar plans for 3-7 beams. A helical 60Co beam geometry achieved similar plan quality as static plans with 11 equidistant 60Co beams. Furthermore, 18 MV plans were initially found not to provide the same target coverage as 6 MV and 60Co plans; however, adjusting the trade-offs in the optimization model allowed equivalent target coverage for 18 MV. For plans with comparable target coverage

  1. Performance of a Roos ionization chamber in gamma radiation beams ({sup 60}Co)

    Energy Technology Data Exchange (ETDEWEB)

    Perini, Ana P.; Neves, Lucio P.; Caldas, Linda V.E., E-mail: lcaldas@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    Among the different types of dosimetry instruments, the ionization chambers are the most practical and and important radiation measurement devices due to their high sensitivity and relatively constant response within a wide range of energies. A commercial PTW ionization chamber (Roos electron chamber) usually utilized in X-ray beams, was tested to verify the possibility of its dosimetric application in {sup 60}Co beams. The main tests in this work were: short- and long-term stability, saturation, ion collection efficiency, polarity effect, leakage current and angular dependence. The characterization tests were performed using a Gammatron {sup 60}Co irradiator and a special goniometer made of PMMA. All results were within international recommendations. The reproducibility test presented results within the recommended limit of {+-}1%, and all coefficients of variation observed in the repeatability test were lower than {+-}0.07%. The ion collection efficiency was better than 99.9% for both polarities. For all pairs of polarity evaluated during the saturation test, the polarity effect was lower than the recommended limit. The maximum variation obtained for angular dependence test was only 0.5%. The chamber tested in this work achieved the expected results in the case of all pre-operational tests realized: stability, leakage current, angular dependence, saturation, ion collection and polarity effect. Evaluating the satisfactory results obtained, it is possible to indicate the usefulness of this ionization chamber for dosimetry in {sup 60}Co gamma radiation beams. (author)

  2. Dosimetric evaluation of Radiotherapy units wit {sup 60}Co; Evaluacion dosimetrica de unidades de radioterapia con {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Leon, B. Salinas de; Tovar M, V.; Becerril V, A. [Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    2000-07-01

    The SSDL network of the IAEA performs, every year, quality audit tests for radiotherapy services ({sup 60} Co units and linear accelerators), and for national SSDL as well. Because of the SSDL-Mexico results in these tests and due to our enthusiasm and confidence in our work, a parallel test has been done , which is described in this talk as well as the results. Nowadays, a second parallel test goes up, which could confirm our optimism and open the possibility to our country to start a national dosimetric audit of {sup 60} Co radiotherapy units. (Author)

  3. Effects of 60Co γ rays on the cell cycle progress of MCF-7 cells

    International Nuclear Information System (INIS)

    To investigate the effects of ionizing radiation on cell cycle progress of tumor cell lines, the human breast cancer MCF-7 cell line cultured in vitro was exposed to 60Co γ rays and the alterations in cell cycle progress after irradiation were measured by flow cytometry. The results indicated that the MCF-7 cells showed a transient S arrest continuing for about 6 h and an obvious G2 arrest continuing for about 63 h after irradiation with 5.0 Gy γ rays. S and G2 arrest culminated at 9 h and 18 h respectively after irradiation and the peak values of S and G2 arrest reached respectively 1.6 times and 6.2 times as many as normal value. The dose-effect curve examined 9 h after irradiation was quite different from that examined 18 h after irradiation. Both of the S arrest at 9 h after irradiation and the G2 arrest at 18 h after irradiation presented significant relationship with irradiation dose

  4. Gamma {sup 60} Co D L {sub 50/30} of Biomphalaria glabrata (Say, 1818)

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Elaine Barros da Costa; Melo, Ana Maria Mendonca de Albuquerque; Motta, Mauricy Alves da [Pernambuco Univ., Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia

    1999-12-01

    The variation of resistance to {sup 60} Co gamma-rays of Biomphalaria glabrata was studied. A population of 480 mollusks was observed during 30 days - distributed in 8 groups of snails isolated and 8 groups of snails in colonies - after exposure (30 snails per group per dose) to increasing doses of gamma radiation. Doses of 10, 20 40, 60, 80, 160, 320 and 640 Gy from a Gamma-cell {sup 60} Co irradiator, were applied to the test groups and two groups control (non-irradiated) of snails - isolated and colony - were kept apart. After have been exposed, the snails were drew back to the aquaria where they were maintained before. The survival was estimated on a daily score of the alive animals in each group-dose, starting after the irradiation exposure day. As a result, the survival self-fertilization forms (DL{sub 50/30}=218.2 Gy) was found greater than in cross-fecundation forms. These data point to allow radio-resistance on the cross-fertilization forms - the sexual reproductive form - which is most found in nature. The lower radio-resistance of the cross-fertilization forms suggests the presence of some sex-linked hormonal factor related to this phenomenon. (author)

  5. Gamma 60 Co D L 50/30 of Biomphalaria glabrata (Say, 1818)

    International Nuclear Information System (INIS)

    The variation of resistance to 60 Co gamma-rays of Biomphalaria glabrata was studied. A population of 480 mollusks was observed during 30 days - distributed in 8 groups of snails isolated and 8 groups of snails in colonies - after exposure (30 snails per group per dose) to increasing doses of gamma radiation. Doses of 10, 20 40, 60, 80, 160, 320 and 640 Gy from a Gamma-cell 60 Co irradiator, were applied to the test groups and two groups control (non-irradiated) of snails - isolated and colony - were kept apart. After have been exposed, the snails were drew back to the aquaria where they were maintained before. The survival was estimated on a daily score of the alive animals in each group-dose, starting after the irradiation exposure day. As a result, the survival self-fertilization forms (DL50/30=218.2 Gy) was found greater than in cross-fecundation forms. These data point to allow radio-resistance on the cross-fertilization forms - the sexual reproductive form - which is most found in nature. The lower radio-resistance of the cross-fertilization forms suggests the presence of some sex-linked hormonal factor related to this phenomenon. (author)

  6. The effect of 60Co γ-rays on Con A and LPS induced lymphocytes

    International Nuclear Information System (INIS)

    The effect to 60Co γ-rays on lymphocytes induced by Con A and LPS and the relationship between these two groups of cells were investigated by means of 3H-TdR incorporation. The study showed that in vitro, Con A cells were able to promote the inducing effect of LPS to B cells. When Con A cells were irradiated by 10 Gy γ-rays, the 3H-TdR incroporation value reduced significanly and the stimulating effect of Con A cells on LPS cells disappeared. Having been irradiated by γ-rays, LPS cells were not able to be stimulated by normal Con A cells. When the groups of cells were incubated together after irradiation, the synergistic function disappeared, furthermore the suppressive effect of Con A cells on LPS cells emerged. When these two groups of cells were investigated by means of agar culture, the suppressive effect of 10 Gy-γ-rays on lymphocytes colony formation was more obvious. Tests on 8 patients who were suffering from carcinoma of nasopharynx showed that after a course of teatment with 60Co γ-rays, the incorporation value in Con A cells became much smaller, the stimulating effect of Con A cells on LPS cells disappeared. LPS cells could not be stimulated by normal Con A cells. The study demonstrated that the radiosensitivity of Con A cells was higher than that of LPS cells

  7. Onset of behavioral effects in mice exposed to 10-Gy 60Co radiation

    Energy Technology Data Exchange (ETDEWEB)

    Maier, D.M.; Landauer, M.R.

    1990-10-01

    The effects of 10 Gray (Gy)60Co radiation on social behavior, locomotor activity, and body weight were assessed in individually housed male Swiss-Webster mice. In Experiment 1, aggressive behavior was evaluated prior to irradiation and for 7 d postirradiation by placing an untreated intruder in the irradiated or sham-irradiated resident's home cage for 5 min. Offensive aggressive behavior was not affected significantly by radiation until day 7 postirradiation, when attack latency increased, the frequency and duration of fighting decreased, and the frequency of bites, lunges, and chases decreased. Untreated intruder mice paired with irradiated resident mice showed a decrease in the duration of defensive upright postures, squeaks, and escapes on day 7 postirradiation. In Experiment 2, locomotor activity and body weight were monitored for 7 d postirradiation. Body weight was decreased in irradiated mice beginning 4 d postirradiation. Locomotor activity was suppressed in irradiated animals 90 min after irradiation and remained depressed throughout the 7-d testing period.

  8. Comparison of treatment in soybean grains between {sup 60}Co and e-beans applications

    Energy Technology Data Exchange (ETDEWEB)

    Fanaro, Gustavo B.; Araujo, Michel M.; Thomaz, Fernanda S.; Duarte, Renato C.; Villavicencio, Anna Lucia C.H. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)]. E-mail: gbfanaro@ipen.br; villavic@ipen.br

    2007-07-01

    Soybean is the most important oleaginous cultivated in Brazil, who is the second largest exported in the world, and generates high incomes, direct and indirectly, its oils could be used since to cook even to machine's fuel and the nutrients become basic for the feeding human being, beyond its by-products, that offer great diversities of products for the nourishing industry. Between the main factors that limit the attainment of high incomes, are the illnesses caused by microorganism like fungi, bacteria, and viruses that, in general, are difficult to control and cause damages on harvest of billions of dollar every year. An alternative to minimize the losses is preserving the grains through the irradiation that can come today from two different main sources: e-beam and {sup 60}Co. Beyond power to be off when it will not be in use, the source of e-beams machines does not need to be recharged, is easily available, possess high tax of dose and low energy. However the {sup 60}Co have low dose rate, high energy and the photons emission is continuous. This work aims to compare the effects of the radiation through viscosimetry, DNA Comet Assay and Cooking time techniques in soybean grains at doses 0, 1.0, 3.0 and 5.0kGy irradiated in ambient temperature at {sup 60}Co source Gammacell 220 (A.E.C. Ltda) and in e-beam accelerator - Radiation Dynamics (Radiation Dynamics Co. model JOB, New York, USA), 1.5 MeV- 25mA with the lower dose. (author)

  9. Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate

    OpenAIRE

    Erma Sulistyaningsih; Sukarti Moeljopawiro; Jarot Subandono; Wayan T. Artama

    2015-01-01

    Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the researchwas to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinanttechnology. Tachyzoite was grown in Balb/c mice in vivo. Messenger RNA was isolated from total RNA and itwas used to synthesis cDNA. Complementary DNA encoding GRA1 protein of tachyzoite Toxoplasma gondii localisolate was amplified and cloned in a prokaryote cloning vector. The recom...

  10. Effect of {sup 60}Co radiation processing in mate (Ilex paraguariensis); Efeito do processamento por radiacao de {sup 60}Co na erva-mate (llex paraguariensis)

    Energy Technology Data Exchange (ETDEWEB)

    Furgeri, Camilo

    2009-07-01

    The mate (Ilex paraguariensis), a native species from South America, is mainly consumed as typical beverage called chimarrao and terere. An important problem that has been afflicting this product since a long time is its natural fungal contamination responsible to affect its physical, health and nutritional qualities. In order to improve this product quality, radiation processing can be effective in reducing pathogens levels, with minimal nutritional and sensory changes. The aim of this study was to evaluate the effectiveness of gamma radiation from {sup 60}Co at doses 0, 3, 5, 7 and 10 kGy in reducing fungal contamination in mate, as well as analyze its nutritional and sensory characteristics. The following methodologies were applied: analysis of yeast and mold, total phenolic compounds analysis, antioxidant analysis, quantification of phenolic compounds and xanthines by liquid chromatography and sensory analysis. Microbiological analysis showed a decreasing molds and yeasts growth with increasing radiation doses. Regardless of the radiation dose applied there were no decrease of total phenolic compounds in both infusions. Chimarrao samples irradiated with 7 and 10 kGy showed a decrease in the DPPH radical-scavenger activity, nevertheless for terere samples, there were no significant difference. Chimarrao chromatographic profile did not show a variation on xanthines quantification, however a 10 kGy radiation dose caused a change to phenolic compounds quantitative profile. Terere samples did not show any significant difference to any analyzed compounds. Sensory analysis did not exhibit a significant difference between irradiated and non irradiated chimarrao samples, as well as between irradiated and non irradiated terere samples. It could be concluded that gamma radiation processing of mate may be a feasible alternative to industry, since there was a reduction on fungal contamination, without changes in sensory qualities and with minimum alterations in quantitative

  11. Radioprotective effects of sodium tungstate on hematopoietic injury by exposure to 60Co γ-rays in Wistar rats

    International Nuclear Information System (INIS)

    Radioprotective effects of sodium tungstate (ST) on 60Co γ-ray induced decrease in hematocrit value and in survival rate in Wistar strain male rats were examined. A long term administration of ST (less than 150 mg/kg body weight/day) for 60-300 day had no significant effects on body and organs weights and survival days. The LD50/60 in 20 weeks old rats was 220 mg/kg body weight/day. Daily administration of 38, 75 or 150 mg from 7 days before and after irradiation to 60 days significantly mitigated the decrease in hematocrit values, especially at 23 days after irradiation (P60Co γ-rays significantly. The dose-reduction factor for survival of 38 mg ST administered rats was 1.14. (author)

  12. Sorption of 60 Co in natural zeolite (clinoptilolite)

    International Nuclear Information System (INIS)

    A Mexican zeolite (clinoptilolite) from Taxco, Guerrero, was partially stabilized with sodium cations. Radioactive Cobalt (60 Co) was used to study the Co 2+ sorption in the stabilized zeolite (Na+). It was found that sorption in general does not favour the diffusion of cobalt between framework, it explains because of it is a natural zeolite and its composition heterogeneous decrease its exchange capacity by the generated competence to the existence other type of exchange ions. The cobalt retention reached the highest level, around 0.408 m eq Co2+ /g in the Na-Clinoptilolite. The crystallinity of the aluminosilicates was maintained during experiments, it was verified by XRD patterns. (Author)

  13. Polymer microcapsules prepared in inverse emulsion by 60Co γ-ray induced interfacial polymerization

    International Nuclear Information System (INIS)

    Inverse emulsion was usually applied to polymerization of hydrophilic monomers, such as acrylamide, acrylic acid, salt of acrylic acid, and N-isopropylacrylamide. However, there have been few reports of one-step synthesis hydrophobic polymer hollow spheres or microcapsules in inverse emulsion system via an interfacial polymerization approach under mild reaction conditions. The motivation of this work is to explore the formation mechanism of polymer microcapsules via γ-ray irradiation in W/O inverse emulsion system. Utilizing the strong reducing radical (OH, hydrated electron) and oxidant radical (eaq-, hydroxyl radical) produced in aqueous phase by 60Co γ-ray irradiation, two interfacial redox initiation systems were proposed and applied to the preparation of polymer microcapsules. In this work, BPO (benzoyl peroxide)-eaq- and DMA (N, N-dimethyl aniline)-OH were used to control the polymerization position at the water-oil interface. High viscosity of external oil phase, high inner phase emulsion and polymeric surfactant were employed to depress the homogeneous nuclei and enhance stability of the inverse emulsion during polymerization. A typical inverse emulsion of water : Span80: kerosene : styrene = 20ml : 2.0g :10ml/4ml (including 0.050g BPO) was irradiated to 20 kGy by 60Co γ-rays at a dose rate of 65 Gy/min. Finally, polystyrene microcapsules were synthesized successfully with an average diameter of 400 nm and shell thickness of 50 nm. The productivity of micro-capsules decreased with the increasing of styrene content due to the homogeneous nuclei. When cyclohexane was used as the oil phase, porous polymer materials can be successfully obtained. Because of the multiplicity in choosing oil-soluble monomers and substances being dissolved in the aqueous phase, this approach revealed interests in encapsulation of bioactive materials or drugs. (authors)

  14. Microbiological analysis of peach palm in natura submitted to {sup 60}Co radiation

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Priscila V.; Araujo, Michel M.; Nunes, Thaise C.F.; Costa, Helbert S.F.; Villavicencio, Anna Lucia C.H. [Instituto de Pesquisas Energeticas e Nucleares (IPEN-CNEN/SP), Sao Paulo, SP (Brazil)], e-mail: priscilavsilva@gmail.com, e-mail: villavic@ipen.br; Hojeije, Khalil Y. [Floresta Industria e Comercio Ltda., Sao Paulo, SP (Brazil)

    2009-07-01

    The palm tree (Bactris gasipaes Kunth) is a species with high potential benefits, because of the nutritional value of its fruits that could be used both in human and animals feeding and mainly for peach palm extraction. It represents a great source of dietary fiber and a moderate source of magnesium and iron. Food irradiation is a worldwide technology that aims to improve the product quality, in order to eliminate diverse microorganisms that can spoil the food. Radiation processing, in the recommended doses, causes very few chemical alterations and nutritional losses in foods, being considered insignificant and/or similar to other food treatments. The objective of this study was to evaluate the effect of irradiation on microbiological counts of mesophilic aerobic in the peach palm in natura. Samples were irradiated with 1.0 and 1.5 kGy using a {sup 60}Co multipurpose irradiator. Radiation treatment appeared to be a useful alternative to reduce microbial contamination in the samples analyzed. (author)

  15. Microbiological analysis of peach palm in natura submitted to 60Co radiation

    International Nuclear Information System (INIS)

    The palm tree (Bactris gasipaes Kunth) is a species with high potential benefits, because of the nutritional value of its fruits that could be used both in human and animals feeding and mainly for peach palm extraction. It represents a great source of dietary fiber and a moderate source of magnesium and iron. Food irradiation is a worldwide technology that aims to improve the product quality, in order to eliminate diverse microorganisms that can spoil the food. Radiation processing, in the recommended doses, causes very few chemical alterations and nutritional losses in foods, being considered insignificant and/or similar to other food treatments. The objective of this study was to evaluate the effect of irradiation on microbiological counts of mesophilic aerobic in the peach palm in natura. Samples were irradiated with 1.0 and 1.5 kGy using a 60Co multipurpose irradiator. Radiation treatment appeared to be a useful alternative to reduce microbial contamination in the samples analyzed. (author)

  16. Gamma 60Co DL50/30 of Biomphalaria glabrata (Say, 1818 DL50/30 raios gama de 60Co em Biomphalaria glabrata (Say, 1818

    Directory of Open Access Journals (Sweden)

    Elaine Barros da Costa CARVALHO

    1999-11-01

    Full Text Available The variation of resistance to 60Co gamma-rays of Biomphalaria glabrata was studied. A population of 480 mollusks was observed during 30 days - distributed in 8 groups of snails isolated and 8 groups of snails in colonies - after exposure (30 snails per group per dose to increasing doses of gamma radiation. Doses of 10, 20, 40, 60, 80, 160, 320 and 640 Gy from a Gamma-cell 60Co irradiator, were applied to the test groups and two groups control (non-irradiated of snails - isolated and colony - were kept apart. After have been exposed, the snails were drew back to the aquaria where they were maintained before. The survival was estimated on a daily score of the alive animals in each group-dose, starting after the irradiation exposure day. As a result, the survival self-fertilization forms (DL50/30 = 218.2 Gy was found greater than in cross-fecundation forms. These data point to a low radio-resistance on the cross-fertilization forms - the sexual reproductive form - which is most found in nature. The lower radio-resistance of the cross-fertilization forms suggests the presence of some sex-linked hormonal factor related to this phenomenon.A variação da resistência entre indivíduos em autofecundação e fecundação cruzada de Biomphalaria glabrata foram estudadas. Uma população de 480 moluscos foi observada durante 29 dias, distribuída em 8 grupos de caramujos isolados e 8 grupos em colônias após a exposição (30 caramujos por grupo-dose a doses crescentes de radiação gama. Foram usadas doses de 10, 20, 40, 60, 80, 160, 320 e 640 Gy de um irradiador Gamma-Cell 60Co. Dois grupos não irradiados - isolado e colônia - foram separados como controle e após a irradiação todos os caramujos voltaram para aquários onde viviam antes. A sobrevida foi estimada pela contagem diária dos animais vivos em cada grupo-dose, a partir do dia da irradiação. O resultado mostrou maior sobrevivência nos grupos isolados (DL50/30 = 218.2 Gy que nos grupos

  17. Study of {sup 60}Co as gamma source in backscatter gamma densitometers

    Energy Technology Data Exchange (ETDEWEB)

    Gholipour Peyvandi, R.; Taheri, A.; Rahmanzadeh Tootkaleh, S.; Askari Lehdarboni, M. [Nuclear Science and Technology Research Institute, Tehran (Iran, Islamic Republic of); Islami Rad, S.Z. [Nuclear Science and Technology Research Institute, Tehran (Iran, Islamic Republic of); Univ. of Qom (Iran, Islamic Republic of). Dept. of Physic

    2015-07-15

    In this work the performance of backscatter gamma densitometer is studied using {sup 60}Co as gamma source. The ability of the densitometer to determine the liquid's interfaces in multi-phase flows was assessed. The aim mainly was to investigate the possibility of using {sup 60}Co in this system. Furthermore, a comparison between {sup 60}Co and was done. The obtained results indicate that {sup 60}Co can be advantageous in this regard.

  18. Retention of ingested (60)Co by a freshwater fish

    International Nuclear Information System (INIS)

    The experiment was carried out on a group of 10 carp receiving the soft tissue of previously contaminated lymnaea as food. Ingestion by carp of 45 daily rations, distributed over a 63-day period, resulted in a low retention of the radionuclide. The contamination kinetics showed that the steady state should be reached after only 225 days. The 60Co transfer factor was approximately 10−2 and the retention factor about 3.3 x 10−3. During the depuration phase, radiocobalt elimination by the carp developed in accordance with an exponential model based on the existence of two biological half-lives of 1.5 and 35 days which indicate a high Co turnover. During both phases of the experiment, urinary and branchial excretion appeared to be higher than faecal excretion

  19. Study on change of free radicals of bamboo with 60Co γ radiation

    International Nuclear Information System (INIS)

    Bamboo samples collected in mountain areas of Fujian province were mechanically pulverized into saw dust. This breaks the covalent bonds of bamboo molecules and free radicals are formed. ESR characterization of the bamboo saw dust samples of 20, 200 and 500 mesh showed that the relative intensity of the 500 mesh saw dust was the highest, and that of the 20 mesh was the lowest(g=2.0033). After 60Co γ-ray irradiation,the intensity of free radicals increased sharply. At>100 kGy, the free radicals tended to be saturated at about 40 times of the free radicals produced mechanically. The intensity of free radicals increased exponentially with the dose below 100 kGy, and decay coefficients of the free radicals in the three samples were different. (authors)

  20. 60Co gamma radiation effect on AlGaN/AlN/GaN HEMT devices

    Institute of Scientific and Technical Information of China (English)

    WANG Yan-Ping; LUO Yin-Hong; WANG Wei; ZHANG Ke-Ying; GUO Hong-Xia; GUO Xiao-Qiang; WANG Yuan-Ming

    2013-01-01

    The testing techniques and experimental methods of the 60Co gamma irradiation effect on AlGaN/AlN/GaN high electron mobility transistors (HEMTs) are established.The degradation of the electrical properties of the device under the actual radiation environment are analyzed theoretically,and studies of the total dose effects of gamma radiation on AlGaN/AlN/GaN HEMTs at three different radiation bias conditions are carried out.The degradation patterns of the main parameters of the AlGaN/AlN/GaN HEMTs at different doses are then investigated,and the device parameters that were sensitive to the gamma radiation induced damage and the total dose level induced device damage are obtained.

  1. Evaluation of fruit productivity and quality in Hass avocado submitted to 60Co gamma radiation

    International Nuclear Information System (INIS)

    Evaluation of productivity, postharvest behavior and fruit quality was performed on four years Hass avocado trees irradiated with 60 Co gamma rays in doses of 5, 10, 15, 20 and 25 Gy, established in the ''La Labor'' Experimental Center of the Centro de Investigaciones Cientificas y Tecnologias del Aguacate en el Estado de Mexico (CICTAMEX) at Temascaltepec Mexico. Productivity had a significant increase in the dose of 15 Gy being the average number of fruits nearly 400 % more than the control at fruit setting, being such difference reduced at fruit harvesting to 300 %. In regard to postharvest performance, the respiration index (mg CO2/kg/hr) did not show significant differences among treatments. Also others variables such as physiological weight losses, texture, maturity pattern, and sensorial tests (color, flavor, aroma, texture) were not different in regard to the control. This mean that radiation has altered productivity but not the quality and postharvest behavior of fruits. (Author)

  2. 60Co gamma radiation effect on AlGaN//AlN/GaN HEMT devices

    International Nuclear Information System (INIS)

    The testing techniques and experimental methods of the 60Co gamma irradiation effect on AlGaN/AlN/GaN high electron mobility transistors (HEMTs) are established. The degradation of the electrical properties of the device under the actual radiation environment are analyzed theoretically, and studies of the total dose effects of gamma radiation on AlGaN/AlN/GaN HEMTs at three different radiation bias conditions are carried out. The degradation patterns of the main parameters of the AlGaN/AlN/GaN HEMTs at different doses are then investigated, and the device parameters that were sensitive to the gamma radiation induced damage and the total dose level induced device damage are obtained. (authors)

  3. Toxoplasma gondii aspartic protease 1 is not essential in tachyzoites.

    Science.gov (United States)

    Polonais, Valerie; Shea, Michael; Soldati-Favre, Dominique

    2011-08-01

    Aspartic proteases are important virulence factors for pathogens and are recognized as attractive drug targets. Seven aspartic proteases (ASPs) have been identified in Toxoplasma gondii genome. Bioinformatics and phylogenetic analyses regroup them into five monophyletic groups. Among them, TgASP1, a coccidian specific aspartic protease related to the food vacuole plasmepsins, is associated with the secretory pathway in non-dividing cells and relocalizes in close proximity to the nascent inner membrane complex (IMC) of daughter cells during replication. Despite a potential role for TgASP1 in IMC formation, the generation of a conventional knockout of the TgASP1 gene revealed that this protease is not required for T. gondii tachyzoite survival or for proper IMC biogenesis.

  4. Five detector set-up for positron 1D-ACAR study of 60Co containing materials

    International Nuclear Information System (INIS)

    Design of the five detector set-up, for 1-Dimensional Angular Correlation of Annihilation Radiation (1-ACAR) study of 60Co-containing materials as well as test measurements are described. The equipment is based on simultaneous registration of a starting positron (by detection of gamma-photon with energy 1274 keV) and its annihilation with an electron in the studied material (by detection of two annihilation gamma photons with the energy 511 keV emitted mainly at the 180o-angle). Through this triple coincidence the distributing 60Co-contribution (gamma-photons with energy of 1.17 and 1.33 MeV) can be effectively separated from the annihilation spectra of irradiated reactor pressure vessel steel specimens. (author). 8 refs, 2 figs

  5. Mechanism of INF-γ antagonizing pulmonary fibroblast proliferation stimulated by 60Co γ-rays

    International Nuclear Information System (INIS)

    Objective: To explore the mechanism of IFN-γ antagonizing human pulmonary fibroblasts (HLF) proliferation stimulated by 60Co γ-rays. Methods: The time- and dose-effect of HLF proliferation promoted by TGF-β1 and rat serum after radiation as well as effect of IFN-γ on proliferation were observed by MTT colorimetric methods. Content of TGF-β1 in rat serum and pulmonary tissues after irradiation as well as the effect of IFN-γ on rat serum after radiation promoting HLF to synthesize TGF-β1 were determined by ELISA. Results: TGF-β1 played a role in promoting HLF proliferation. The sera of irradiated rats could promote HLF proliferation and TGF-β1 synthesis, but the effect was inhibited by IFN-γ significantly (P<0.05). The level of TGF-β1 in rat serum and lung tissues gradually increased from 1 to 4 weeks after irradiation. Conclusions: TGF-β1 is involved in the process of γ-rays stimulating HLF proliferation, while IFN-γ can inhibit HLF from synthesizing TGF-β1 and antagonize its role of promoting HLF proliferation. (authors)

  6. Succinylcholine-induced hyperkalemia in the rat following radiation injury to muscle. [60Co

    Energy Technology Data Exchange (ETDEWEB)

    Cairoli, V.J.; Ivankovich, A.D.; Vucicevic, D.; Patel, K.

    1982-02-01

    During anesthetic preparation of a patient who had received routine radiation therapy of sarcoma of the leg, cardiac collapse occurred following succinylcholine (SCh) administration. Experiments were designed to test the hypothesis that radiation injury to muscle might cause increased sensitivity to SCh similar to that reported in patients with muscle trauma, severe burns, and lesions causing muscle denervation. Venous plasma potassium levels and arterial blood gas tensions were measured in rats after they were given SCh (3 mg/kg) at various times following 60Co irradiation of the hind legs. Nonirradiated rats responded to SCh with a slight but statistically significant increase in plasma K+. Rats subjected to high levels of radiation (10,000 to 20,000 R) and given SCh 4 to 7 days later responded in the same way as the control rats. Plasma K+ levels in rats exposed to a fractionated irradiated dosage (25000 R given twice with a 1-week interval) followed by SCh 1 week later were similar to those in the control group, but when SCh was given 2 weeks later (3 weeks after initial irradiation) there was a marked elevation of plasma K+, from 3.6 to 7.7 meq/L, a statistically significant increase.

  7. Protective effect of catecholestrogens against 60Co γ-ray radiation injury in mice

    International Nuclear Information System (INIS)

    When 10-week-old male BALB/c mice received whole-body irradiation with a single dose of 8 Gy 60Co γ-ray and catecholestrogen 2-hydroxyestradiol (2-OHE2) was subcutaneously injected 3 hours before and after the irradiation, 30-day survival rate of the mice was 70%, while the survival rate of the mice administered the other test samples was as follows; 2-hydroxyestrone, 20%; 2-hydroxyestriol, 20%; 4-hydroxyestradiol, 0%; 2-methoxyestrone, 0%; 2-methoxyestradiol, 0%; 2-methoxyestriol, 0%; estrone, 0%; estradiol, 5%; estriol, 0%; control, 5%. Lipid peroxide level in the liver of mice markedly increased on the 4th day after the irradiation. However, this increase in the lipid peroxide level was significantly suppressed by the administration of 2-OHE2. The radiation-induced anemia, leukopenia, and atrophy of the thymus were significantly protected by the administration of 2-OHE2. These results indicate that 2-OHE2 had a potent inhibitory effect on radiation injury. (author)

  8. Transference kinetics of 60Co in an aquatic-terrestrial ecosystem

    Institute of Scientific and Technical Information of China (English)

    ZHAO Xiyue; CAI Zhiqing; GONG Fanghong; SHI Jianjun; WANG Shouxiang

    2008-01-01

    The dynamics of transportation,accumulation,disappearance and distribution of 60Co in a simulated aquatic-terrestrial ecosystem was studied by isotope-tracer technique.In the aquatic system,60Co was transported and transformed via depositing,coupling with ions and adsorption.The absorption resulted in the redistribution and accumulation of 60Co in each compartment of the system.Specific activities of 60Co in water started sharply and gently decreased.The sediment accumulated a large amount of 60Co by adsorption and ion exchange.The homwort (Ceralophyllum demersum) could also adsorb a large amount of 60Co in a short time,because of its large specific surface area.Fish (Carassius auratus) and snail (Bellamya purificata) had a poor capacity of adsorbing 60Co.The distribution of 60Co in the fish was mainly in the viscera,and the amount of 60Co in the snail flesh was greater than that in the shell.The amount of 60Co in individual compartment in the system was changed with time.The highest specific activity of 60Co in the bean of the terrestrial system remained in the root nodule.

  9. The radiation effect of 60Co gamma rays on polycarbonate detector

    International Nuclear Information System (INIS)

    The irradiation of polymeric materials with ionizing radiation (gamma rays, X rays, accelerated electrons, ion beams) leads to the formation of very reactive intermediates products (excited states, ions and free radicals), which result in rearrangements and/or formation of new bonds. The effects of these reactions are formation of oxidized products, grafts, scission of main chain (degradation) or cross-linking. Often the two processes (degradation - cross- linking) occur simultaneously, and the outcome of the process is determined by a competition between the reactions. Polycarbonate detectors are used as a particle track detector for neutrons and alpha particles detection. This work aims to study the ionizing radiation dose response of polycarbonate samples using spectrophotometric technique. A commercially available polycarbonate was analysed and its dosimetric characteristics were studied: radiation-induced absorption spectra, ambient light, temperature and humidity influence, pre- and post-irradiation stability, reproducibility and dose range useful response. Samples of polycarbonate (3 x 1 cm2) were irradiated with 60Co gamma radiation in free air at electronic equilibrium with absorbed doses between 1 and 95 kGy. When exposed to gamma radiation the polycarbonate detectors undergoes changes in their optical response, the colour variation is used for determining the absorbed dose. A Shimadzu UV-2101PC spectrophotometer was used for scanning the absorption spectra and measuring the optical density of film detectors irradiated with different radiation doses. Polycarbonate film detector are easy to prepare and to analyse, of good optical quality, inexpensive and of small size. The dosimetric accuracy can be affect by environmental conditions so, the detectors must be stored in appropriate conditions. The reproducibility of the detectors response can be improved by careful monitoring of optical densities before irradiation. The dose response curve presents linear

  10. A Study on the Characters of the Seeds of M2 Generation of Chamaecrista Freated with 60Coγ-ray Irradiation%红壤山地决明属牧草辐射后代变异趋势的研究

    Institute of Scientific and Technical Information of China (English)

    徐国忠; 郑向丽; 应朝阳; 黄毅斌; 翁伯琦

    2005-01-01

    利用60Coγ射线(辐照剂量为300Gy ,剂量率为4.6875Gy)辐照豆科决明属5个牧草品种的种子,通过盆栽混合收集M1代各个品种的种子,然后进行M2代试验观察和测定,了解其变异趋势.辐射M2代试验结果表明,辐照后5个决明品种的出苗率、茎长、叶宽、地上部干重都无显著差异;冠高34721有显著增长、茎粗2219有显著增长、分枝数86134有极显著增长和92985有显著降低、叶数86134有显著增长、叶长2219有显著增长;植株的营养成分(N、P、K),全(K)2219有显著下降;植株的营养成分(氨基酸),2219氨基酸总的含量有显著下降、且17种氨基酸都表现为不同程度的下降,86134氨基酸总的含量有显著增长、且17种氨基酸都表现为不同程度的增长.

  11. TGFβ1正、反义基因转染60Co照射HELF对TGFβ1及I型前胶原mRNA表达调控影响%Gene transfection using lipid-mediated TGFβ1 sense and antisense gene expression vectors and its effects on TGFβ1 and procollagen I mRNA expression in 60Co-irradiated human embryo lung fibroblasts

    Institute of Scientific and Technical Information of China (English)

    刘纯杰; 王德文; 高亚兵; 熊呈琦; 彭瑞云; 崔雪梅; 宋良文; 张兆山; 龙建银; 王会信

    2001-01-01

    Objective To investigate the effects on gene expression of 60Co-irradiated human embryo lung fibroblasts after gene transfection using lipid-mediated TGFβ1 sense and antisense gene expression vectors. Methods TGFβ1 sense and antisense gene expression vectors were transfected using a lipid-mediated method.Gene expression was analysed by RNA dot blot. Results HELFs irradiated with 5 Gy were transfected with an expression vector encoding the human TGFβ1 sense or antisense gene under control of the mouse mammary tumor virus long terminal repeat(MMTV-LTR)promotor/enhance sequence(pMAMneo-TGFβ1,or pMAMneo-anti-TGFβ1).The transfected cells elected by G418 resistance were cultured in DMEM containing dexamethasone.The chromosomal DNA and RNA were extracted.Positive reaction was showed from chromosomal DNA by a PCR method of neo-specific primers and DNA dot blot with Dig-labelling neo-specific probe.RNA dot blot analysis showed that TGFβ1 mRNA level of the cells transfected with pMAM neo-anti TGFβ1 decreased,but that of transfected with pMAM neo-TGFβ1 increased.For procollagen Ⅰ mRNA,the transfected pMAM neo-anti TGFβ1 was lower than untransfected cells and the transfected pMAM neo-TGFβ1 was higher. Conclusion After TGFβ1 sense and antisense gene transfection,TGFβ1 mRNA level of the cells transfected with TGFβ1 antisense gene decreased,but that with TGFβ1 sense gene increased.For procollagen Ⅰ mRNA,the cells transfected with TGFβ1 antisense gene was lower than untransfected cells and the cells transfected with TGFβ1 sense gene was higher than untransfected cells.%目的观察TGFβ1正、反义基因转染60Co γ射线照射的HELF后对其TGFβ1 mRNA及Ⅰ型前胶原mRNA表达调控的影响。方法采用脂质体介导法进行基因稳定转染,转染细胞经PCR,DNA dot blot鉴定和RNA dot blot分析。结果选择5 Gy照射细胞,采用Lipofect AMINE将TGFβ1正、反义基因表达载体pMAMneo-TGFβ1和pMAMneo-Anti TGFβ1

  12. 60 Co 放射源运输容器屏蔽性能检测%Shielding Performance Measurements on Container for 60 Co Radioactive Sources Transport

    Institute of Scientific and Technical Information of China (English)

    孙洪超; 李国强; 闫峰; 庄大杰; 孙树堂; 王学新

    2015-01-01

    工业及医疗用放射源主要包括60 Co、137 Cs、131 I、32 P、153 Sm、99 Mo、90 Sr、89 Sr等核素,射线形式有α、β、γ、中子等。本文针对运输活动中γ辐射,使用现有的60 Co放射源运输容器,开展辐射屏蔽性能检测技术研究。通过模拟计算和实验测量,得到运输容器最大装载活动情况下外部辐射水平,并对计算和实验结果进行了比较。针对放射源在屏蔽容器中安放位置发生偏移和放射源在容器中安放方式不同对容器外部的辐射水平影响进行了相关研究。研究结果可对今后完善放射性物质运输容器的辐射屏蔽性能检测提供一定的借鉴。%Some radioactive sources are often used in irradiation industry and radiothera‐py ,such as 60Co ,137Cs ,131I ,32P ,153Sm ,99Mo ,90Sr ,89Sr and so on .The radiation includeα、β、γ and n .The radiation from γ sources are the mostly concerned during the transporting of radioactive sources .The shielding performance measurements on con‐tainer for 60Co radioactive sources transport were discussed by using both Monte Carlo method simulation calculation and experiment measurement . Some important factors w as discussed w hich could affect the results of shielding performance measurements . The layout of radioactive sources had a important impact on the monitoring results of the external radiation levels of radiation sources transport container .The results affor‐ded the contribution to the improvement of the radiation monitoring and management of radioactive material transportation package .

  13. ONE CASE OF LEUKEMIA INDUCED BY 60Co ACCIDENT

    Institute of Scientific and Technical Information of China (English)

    邵松生; 冯嘉林; 等

    1995-01-01

    The peripheral blood lymphocyte chromosomes in a case of 60Co γ rays accident were examined at 2.5h after exposure.The frequency of the dicentrics plus centric rings was 89% and exposure dose was estimated to be 4.78 (4.53-4.88)GY.The examinations of lymphocyte chromosome aberration within follow-up 12a showed that the incidence of Dic+R reduced with lg regrssion(r=-0.9895).While the number of ceels with stable aberration remained unchanged and showed a tendency to increase.During the period of leukemia,bone marrow cell chromosome aberrations were studied by method of G-banding.Of 13 cells observed,4 cells belonged to normal karyotypes.Among 9 aberration cells,12 aberrations were detected.The majority of which were classified as translocation,deletion and inversion,Numeric aberrations were-9,12,20-,22,-y.This case suggested that acute lymphocytic leukemia was induced after radiation accident.

  14. Effect of gamma radiation of 60Co in the variability of chinese bean [Vigna unguiculata (l.) Walpers] en R4M4

    International Nuclear Information System (INIS)

    Selection of plants of Chinese bean coming from seed irradiated with gammas of 60Co in the generation R4M4 (fourth recurrent irradiation, fourth segregate generation) were carried out, taking as selection approaches the plant architecture, the numbers of sheaths for plant, sheath longitude, position of the sheaths, grain size and resistance to plagues and illnesses. 17 lines were selected for grain and 3 lines with fodder characteristics of black grain color were also obtained. (Author)

  15. Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate

    Directory of Open Access Journals (Sweden)

    Erma Sulistyaningsih

    2015-10-01

    Full Text Available Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the researchwas to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinanttechnology. Tachyzoite was grown in Balb/c mice in vivo. Messenger RNA was isolated from total RNA and itwas used to synthesis cDNA. Complementary DNA encoding GRA1 protein of tachyzoite Toxoplasma gondii localisolate was amplified and cloned in a prokaryote cloning vector. The recombinant GRA1-encoding gene was thendigesting using EcoRI restriction endonuclease and sequencing. The result showed that the recombinant GRA1-encoding gene consisted of DNA sequences encoding all signal peptide and mature peptide of GRA1 protein.Alignment of recombinant GRA1 sequence to gene encoding GRA1 protein of Toxoplasma gondii RH isolate showed100% homologous.Keywords: GRA1 protein, Toxoplasma gondii, tachyzoite, cloning, cDNA

  16. Toxoplasma gondii: demonstration of intrinsic peroxidase activity during lacto-peroxidase mediated radioiodination of tachyzoites

    Energy Technology Data Exchange (ETDEWEB)

    Gallois, Y.; Tricaud, A.; Foussard, F.; Hodbert, J.; Girault, A.; Mauras, G.; Dubremetz, J.F.

    1986-01-01

    Tachyzoites of Toxoplasma gondii have been radioiodinated under various conditions with or without lactoperoxidase, with glucose oxidase being used to generate hydrogen peroxide. Erythrocytes were iodinated simultaneously as a control. In our conditions, tachyzoites were more intensely labelled in the absence of lactoperoxidase. This result can be explained by the existence of an intrinsic peroxidase activity which interfere with the exogenously added enzyme during surface radioiodination.

  17. A standard Fricke dosimeter compared to an ionization chamber used for dosimetric characterization of 60Co photon beam

    Science.gov (United States)

    Moussous, Ouiza; Medjadj, Toufik

    2016-06-01

    The main objective of this study was to investigate the Fricke dosimeter water equivalent system for measurement of dosimetric parameters for photon beam. The parameters measured with the Fricke dosimeter were compared to those obtained with an ionization chamber. In this work characteristics for 60Co γ-rays of field sizes ranging from 5 × 5 cm2 to 20 × 20 cm2 are reported. The measurements were carried out in the secondary standard dosimetry laboratory using a collimated 60Co gamma source therapy unit. The 60Co beam output in terms of absorbed dose to water was obtained as per IAEA TRS 398 recommendations using cylindrical ionization chamber, whose ND,w has been supplied by the IAEA's reference laboratory. Specific quantities measured include: output factors, peak scatter factor, lateral beam profiles and percentage depth dose. The Fricke dosimeters were irradiated in a water phantom using the suitable poly (methyl methacrylate), PMMA stand. Our results demonstrate that Fricke dosimeter and ionization chamber agree with each other.

  18. Radiation effects of 60Co γ-rays on expression of CDKN1A mRNA in human lymphocytoblast

    International Nuclear Information System (INIS)

    Objective: To study effect of 60Co γ-rays radiation with different doses on expression of CDKN1A gene mRNA in human lymphocytoblast cultured for different time. Methods: After human lymphocytoblasts were irradiated by the 60Co γ-rays with various doses of 0, 0.2, 1, 3, 5 and 10 Gy, and cells were separately cultured for sustaining survival during 0, 4, 24, 48, 72 and 96 h. The total RNA was extracted from each sample and the real-time PCR was conducted to observe the gene CDKN1A mRNA level changes in lymphocytoblasts exposed to various radiation doses for various cultured time. Results: Expressive levels of the CDKN1A mRNA in lymphocytoblasts gradually went up with increasing radiation doses, which showed γ-rays dose dependent from 0 Gy to 5 Gy (P<0.05), and reach the peak when cells were cultured for 24 h after exposing to radiation while displayed an expressive downtrend during the later stage of cell culture. Conclusion: Increase of the CDKN1A mRNA expression level in human lymphocytoblasts after exposing to 60Co γ-rays radiation within 24 hours of culture shows a dose dependent way, which may be used to evaluate the ionizing radiation dose. (authors)

  19. Study on the grafting of N-vinypyrrolidone onto xanthan gum by 60Co γ-irradiation and properties of the grafted copolymer%黄原胶辐射接枝N-乙烯基吡咯烷酮及其物性研究

    Institute of Scientific and Technical Information of China (English)

    李咏富; 李彦杰; 哈益明; 王锋

    2011-01-01

    Simultaneous radiation grafting method (60Co γ-rays as the radiation source) was used to prepare xanthan gum-graft-N-vinypyrrolidone (XG-g-NVP), and the influence factors of the grafting degree was preliminarily studied. The suucture of the grafted copolymer was characterized by the Fourier-transform infrared spectroscopy (FT-IR) and thermo gravimetric analysis (TGA), and the solution viscosity properties of the grafted copolymer in low grafting rate were studied by rheometer. The results show that the grafting rate increases with absorbed dose and gradually achieve to the balance. The grafting rate reaches maximum when the xanthan gum concentration is 10 g/L or the mass ratio is 10:1 meanwhile the other two factors are in a certain experimental value range. FT-IR figure shows that the grafting reaction occurs in NVP vinyl part and lactam structure is retained. Thermo-gravimetric analysis shows that XG-g-NVP enhanced thermal stability of xanthan gum. The properties of XG-g-NVP solution show that grafting helps to enhance the performance of resisting high temperature and shear strength.%采用共辐射法制备了黄原胶-N-乙烯基吡咯烷酮接枝共聚物(XG-g-NVP).研究表明在黄原胶浓度及XG/NVP质量比一定的情况下,接枝率随吸收剂量的增加而增加,并逐步达到平衡:在吸收剂量和质量比一定时,接枝率住浓度为10g/L达到最大;在吸收剂量和黄原胶浓度一定时,接枝率在质量比为10:1达到最大值.红外光谱研究表明接枝反应发生在NVP乙烯基部位,接枝共聚物保留了NVP的内酰胺结构;热重分析表明接枝共聚物的热稳定性得到了增强.接枝共聚物在低接枝率下溶液粘性特征的流变分析表明,其抗高温、抗剪切的能力得到了提高.

  20. Use of {sup 60}Co panoramic source in the induction of oral mucositis in rats

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Maira F.; Benetti, Carolina; Zezell, Denise M., E-mail: mairandrade@yahoo.com, E-mail: zezell@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Correa, Luciana, E-mail: lcorrea@usp.br [Universidade de Sao Paulo (FO/USP), Sao Paulo, SP (Brazil). Fac. de Odontologia

    2013-07-01

    Oral Mucositis is a well-known side effect of chemo-radiotherapy in cancer patients or transplant recipients that could induce hospitalization or impairs therapy in different levels of severity. This study is devoted to define the first steps in the research of low level laser treatments in oral mucositis, proposing a {sup 60}Co radiation to experimentally induce oral mucositis in rats using Panoramic gamma irradiator, simulating usual radiotherapy of head and neck cancer. Fifteen male Wistar rats, above 250g, were irradiated at Centro de Tecnologia das Radiacoes (IPEN - CNEN/SP) and divided in three experimental groups, with different single doses of radiation (30 Gy, 25 Gy and 20 Gy). The animals were observed for a 20 days period. Animals that received 30 Gy and 25 Gy developed greater severity of mucositis and premature euthanasia was performed in these groups on the 7th and 11th day after the irradiation, respectively. The 20 Gy group developed oral mucositis grading from moderated to severe between the days 7 and 11 after irradiation, with progressive body mass loss and decrease in the intake of food and water. These animals recovered from oral mucositis around the 18th day and clinical remission at the 20th day. The single dose of 20 Gy Gamma radiation proved to be efficient way for inducing oral mucositis in rats, allowing the establishment of an experimental model for oral mucositis in rats for future use on interventions of this serious aspect of radiation therapy, such as laser therapy using different wave lengths and power densities. (author)

  1. Use of 60Co panoramic source in the induction of oral mucositis in rats

    International Nuclear Information System (INIS)

    Oral Mucositis is a well-known side effect of chemo-radiotherapy in cancer patients or transplant recipients that could induce hospitalization or impairs therapy in different levels of severity. This study is devoted to define the first steps in the research of low level laser treatments in oral mucositis, proposing a 60Co radiation to experimentally induce oral mucositis in rats using Panoramic gamma irradiator, simulating usual radiotherapy of head and neck cancer. Fifteen male Wistar rats, above 250g, were irradiated at Centro de Tecnologia das Radiacoes (IPEN - CNEN/SP) and divided in three experimental groups, with different single doses of radiation (30 Gy, 25 Gy and 20 Gy). The animals were observed for a 20 days period. Animals that received 30 Gy and 25 Gy developed greater severity of mucositis and premature euthanasia was performed in these groups on the 7th and 11th day after the irradiation, respectively. The 20 Gy group developed oral mucositis grading from moderated to severe between the days 7 and 11 after irradiation, with progressive body mass loss and decrease in the intake of food and water. These animals recovered from oral mucositis around the 18th day and clinical remission at the 20th day. The single dose of 20 Gy Gamma radiation proved to be efficient way for inducing oral mucositis in rats, allowing the establishment of an experimental model for oral mucositis in rats for future use on interventions of this serious aspect of radiation therapy, such as laser therapy using different wave lengths and power densities. (author)

  2. Change of free radicals and chemical structure of Moso bamboo with 60Co γ radiation

    International Nuclear Information System (INIS)

    After 60Co γ radiation, absolutely dry bamboo powder produced free radicals and its chemical composition and structure changed. Electron spin resonance (ESR) and X-ray photoelectron spectroscopy (XPS) techniques were used to measure the spectrum of the free radicals and X-ray photoelectron spectroscopy of the γ-ray radiated bamboo powder. The test results reveal that when the spectral splitting factor (g) of the bamboo free radicals is 2.0033, and the intensity of free radicals increase with the absorbed dosage of irradiation according to exponential law. After irradiation of 200 kGy, the O/C atomic number ratio in the bamboo surface increases slightly, C-C and C-H content increases, C-O and C=O content decreases, and -O-C=O content increases to 1.5 times over that of the original, which indicates some oxygen-containing functional groups occur and oxidation state of carbon increases in the bamboo surface. (authors)

  3. Evaluation of gamma-sterilization ({sup 60}Co) by RT-PCR by DHFR expression detection

    Energy Technology Data Exchange (ETDEWEB)

    Converso, Ana Paula G.; Andrade Junior, Heitor F. de [Instituto de Medicina Tropical de Sao Paulo, Sao Paulo, SP (Brazil)]. E-mail: anapaulagconverso@gmail.com; hfandrad@usp.br; Vieira, Daniel P. [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Instituto de Medicina Tropical de Sao Paulo, Sao Paulo, SP (Brazil); E-mail: dperezv@usp.br

    2007-07-01

    The improvement of techniques to detect pathogen agents in blood had reduced significantly the contamination mechanisms by hemocomponents in blood transfusion procedures. Ionizing radiation is a method that has presented several applications on medicine and in currently days has been showing special attention on blood banks which has been applied to avoid TA-GVHD development. DHFR is an enzyme constitutive in Plasmodium protozoa and has an important role in folate metabolism on these parasites. Detecting the expression of RNAm coder for this enzyme is possible to evaluate the viability of this parasite in blood samples. Plasmodium chabaudi AJ is a parasite that induces lethal malaria in rodents similar to human malaria In this work, the objective was to detect the presence of plasmodium protozoa in irradiated blood samples, infected experimentally, through the application of a RT-PCR using primers for the coder sequence of DHFR's mRNA. We studied doses of ionizing radiation between 0 and 75 Gy. The irradiation procedures were accomplished in Center of Radiation Technology of IPEN-CNEN in a {sup 60}Co panoramic source. Our results had demonstrated that RT-PCR is a sensible method to evaluate the viability of plasmodium in blood samples because the technique could detect low parasite burden in all tested samples. (author)

  4. Determination of late-time Gamma-Ray (60Co) sensitivity of single diffusion Lot 2N2222A transistors.

    Energy Technology Data Exchange (ETDEWEB)

    DePriest, Kendall Russell; Kajder, Karen C.; Peters, Curtis D. (American Staff Augmentation Providers, LLC, Albuquerque, NM)

    2008-08-01

    Sandia National Laboratories (SNL) has embarked on a program to develop a methodology to use damage relations techniques (alternative experimental facilities, modeling, and simulation) to understand the time-dependent effects in transistors (and integrated circuits) caused by neutron irradiations in the Sandia Pulse Reactor-III (SPR-III) facility. The development of these damage equivalence techniques is necessary since SPR-III was shutdown in late 2006. As part of this effort, the late time {gamma}-ray sensitivity of a single diffusion lot of 2N2222A transistors has been characterized using one of the {sup 60}Co irradiation cells at the SNL Gamma Irradiation Facility (GIF). This report summarizes the results of the experiments performed at the GIF.

  5. Bioaccumulation and elimination of 60 Co and 137 Cs by Anomalocardia brasiliana (Gmelin, 1791) (Mollusca bivalvia). Remobilization of 60 Co, retained in marine sediment by microbial activity

    International Nuclear Information System (INIS)

    It was studied the capacity of a bi valve mollusc Anomalocardia brasiliana, which lives in the local bottom sediment, to remobilize 60 Co previously sorbed in the sediment. Laboratory experiments demonstrated that the transference of 60 Co from the sediment to the animal was insignificant (bioaccumulation factor (BF) of the order of 10-3). At the same time, the capacity of microorganisms, present in the bottom sediment, to remobilize 60 Co was studied. The results showed that this via of transference was important, considering the much greater microorganism biomass in relation to the biomass of bentonic organisms, as a whole. For 137 Cs the determined BF from water to the animal was 2.2. and, as in the case of 60 Co, the soft tissues concentrated more 137 Cs than shell. Remaining viscera showed the highest BFs. In another series of experiments, the loss of 60 Co or 137 Cs, previously accumulated by A. brasiliana, was followed in aquaria with or without sediment and the respective biological half-lives were calculated. Soft tissues retained 60 Co longer (biological half-life = 117 days) than shells, whereas for 137 Cs the opposite was observed and shells showed a biological half life of 38.5 days. The low values of 60 Co and 137 Cs BFS do not allow to classify A. brasiliana as good biological indicator for pollution by there radionuclides. However since A brasiliana is consumed by the local population and is commercialized to other areas, it was recommended that its contamination by 60 Co or 137 Cs should be monitored. (author)

  6. Determination of the intrinsic energy dependence of LiF:Mg,Ti thermoluminescent dosimeters for {sup 125}I and {sup 103}Pd brachytherapy sources relative to {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Reed, J. L., E-mail: jlreed2@wisc.edu; Micka, J. A.; Culberson, W. S.; DeWerd, L. A. [Department of Medical Physics, University of Wisconsin–Madison, Madison, Wisconsin 53705 (United States); Rasmussen, B. E. [Department of Radiation Oncology, UP Health System Marquette, 580 West College Avenue, Marquette, Michigan 49855 (United States); Davis, S. D. [Department of Medical Physics, McGill University Health Centre, Montreal General Hospital (L5-112), 1650 Cedar Avenue, Montreal, Quebec H3G 1A4 (Canada)

    2014-12-15

    Purpose: To determine the intrinsic energy dependence of LiF:Mg,Ti thermoluminescent dosimeters (TLD-100) for {sup 125}I and {sup 103}Pd brachytherapy sources relative to {sup 60}Co. Methods: LiF:Mg,Ti TLDs were irradiated with low-energy brachytherapy sources and with a {sup 60}Co teletherapy source. The brachytherapy sources measured were the Best 2301 {sup 125}I seed, the OncoSeed 6711 {sup 125}I seed, and the Best 2335 {sup 103}Pd seed. The TLD light output per measured air-kerma strength was determined for the brachytherapy source irradiations, and the TLD light output per air kerma was determined for the {sup 60}Co irradiations. Monte Carlo (MC) simulations were used to calculate the dose-to-TLD rate per air-kerma strength for the brachytherapy source irradiations and the dose to TLD per air kerma for the {sup 60}Co irradiations. The measured and MC-calculated results for all irradiations were used to determine the TLD intrinsic energy dependence for {sup 125}I and {sup 103}Pd relative to {sup 60}Co. Results: The relative TLD intrinsic energy dependences (relative to {sup 60}Co) and associated uncertainties (k = 1) were determined to be 0.883 ± 1.3%, 0.870 ± 1.4%, and 0.871 ± 1.5% for the Best 2301 seed, OncoSeed 6711 seed, and Best 2335 seed, respectively. Conclusions: The intrinsic energy dependence of TLD-100 is dependent on photon energy, exhibiting changes of 13%–15% for {sup 125}I and {sup 103}Pd sources relative to {sup 60}Co. TLD measurements of absolute dose around {sup 125}I and {sup 103}Pd brachytherapy sources should explicitly account for the relative TLD intrinsic energy dependence in order to improve dosimetric accuracy.

  7. Effects of γ (60Co) and β (90Sr) radiations in Chinese hamster ovarian cells (CHO-K1): induction of micronuclei and cell death

    International Nuclear Information System (INIS)

    Among various types of ionising radiation, the beta emitter radionuclides are involved in many sectors of human activity, such as nuclear medicine, nuclear industries and biomedicine, with a consequent increased risk of accidental, occupational or therapeutic exposure. Despite their recognized importance, there is little information about the effect of beta particles at the cellular level when compared to other types of ionizing radiation. Thus, the objective of the present study was to evaluate the genotoxic and cytotoxic effects of 90Sr, a pure, highly energetic beta source, on CHO-K1 cells and to compare them with data obtained with 60Co. CHO-K1 cells irradiated with different doses (1.0, 2.5, 5.0, 7.5 Gy) of 60Co (0.34 Gy.min-1) and 90Sr (0.23 Gy.min-1) were processed for analysis of clonogenic death, induction of micronuclei (MN) and necrotic and apoptotic death. The survival curves obtained for both types of radiation were better fitted by the linear-quadratic model and were similar. However, the cytogenetic results showed that both the proportion of micronucleated cells and the magnitude of radioinduced lesions demonstrated by the analysis of MN distribution were significantly higher in cells irradiated with 60Co than in cells irradiated with 90Sr, whereas 90Sr was more damaging than 60Co in terms of cell death induction. Necrosis was the major type of death observed in CHO-K1 cells. The data obtained suggest that the low incidence of micronucleated cells after exposure to 90Sr may be a consequence of selective elimination of severely damaged cells from the population by the necrotic process at a higher rate than observed with 60Co exposure. The data obtained also demonstrated the need to use several parameters for a better estimate of cellular sensitivity to the action of genotoxic agents, which would be important in terms of radiobiology, oncology and therapeutics. (author)

  8. Effect of gamma radiation of {sup 60}Co in the variability of chinese bean [Vigna unguiculata (l.) Walpers] en R{sub 4}M{sub 4}; Efecto de la radiacion gamma de {sup 60}Co en la variabilidad de frijol chino [Vigna unguiculata (l.) Walpers] en R{sub 4}M{sub 4}

    Energy Technology Data Exchange (ETDEWEB)

    Salmeron E, J.; Bueno J, J.E.; Valencia E, F. [CSAEGro, Iguala, Guerrero, Tel and Fax 01 733 (33) 24328 (Mexico); Cervantes S, T. [IREGEP (Mexico); Cruz T, E. De la [ININ, Departamento de Biologia, 52750 La Marquesa, Estado de Mexico (Mexico)]. e-mail: csaegro@prodigy.net.mx

    2007-07-01

    Selection of plants of Chinese bean coming from seed irradiated with gammas of {sup 60}Co in the generation R{sub 4}M{sub 4} (fourth recurrent irradiation, fourth segregate generation) were carried out, taking as selection approaches the plant architecture, the numbers of sheaths for plant, sheath longitude, position of the sheaths, grain size and resistance to plagues and illnesses. 17 lines were selected for grain and 3 lines with fodder characteristics of black grain color were also obtained. (Author)

  9. Effects of 60Co gamma radiation on defense function of human polymorphonuclear leukocytes

    International Nuclear Information System (INIS)

    The effects of radiation on defense function of polymorphonuclear leukocytes (PMN) were studied following irradiation with 60Co γ radiation (30 - 3,000 rad) using PMN separated from the peripheral blood of healthy volunteers. The migration distances for all three measures of chemotaxis to fMet-Leu-Phe (10-8 M), chemokinesis induced by fMet-Leu-Phe, and random migration tended to decrease with increasing dose, showing 0.0054 μm/rad (p -5 M) in conjunction with cytochalasin B (CB, 5 μg/ml) there was a significant dose trend, showing the dose effects of decreasing 0.0022 % release/rad for BGL and 0.0030 % release/rad for LYZ with increasing dose. In superoxide anion (O2-) production, a slight and marginally significant linear dose trend was found. These results suggest that the defense function of PMN is not so resistant to radiation as predicted from the fact that PMN in the peripheral blood are differentiated and mature. It is thought that radiation inflicts substantially harmful effects on the defense function of peripheral PMN. (author)

  10. Some gamma radiation (60Co) effects on Chrysomya megacephala (Fabricius, 1794) (Diptira-Calliphoridae)

    International Nuclear Information System (INIS)

    The effect of different gama radiation (60Co) doses on Chrysomya megcephala (Fabricius) have been studied under laboratory conditions, in the Entomology Section of Centro de Energia Nuclear na Agricultura (CENA-USP), Piracicaba, SP, Brasil. The insects were collected close to sucking animals brooding areas, identified and reared on artificial diet, being kept in a temperature (26 +- 20C) and humidity (66 +- 9%) controlled room, with 12L:12D photopheriod. A Gammabeam type 650 Cobalt-60 gamma irradiation source was used, with 28, 196x1010 Bq activity at the beginning of the experiment. The pupae were submitted to increasing gamma radiation doses. To determine lethal doses, treatments were carried out at four different ages and to determine the sterilizing doses two-three day pupae were used. It was noted pupae radiosensitivity decreased with age, LD50 varying from 14 - 513 Gy. The sterilizing dose for males was 40 Gy and for females 30 Gy. During the individuals life cycle, it was noted that there was a decrease in life expectancy (exo) of adults when the gamma radiation dose was increased. Treated males were more sensitive than females (Crossed with ''normals''), there occurring a marked reduction in the number of eggs and their viability when the used gamma radiation dose was increased. (author)

  11. Modification of polyethersulfone films by grafting hydrophilic monomers with 60Co γ-rays

    International Nuclear Information System (INIS)

    Polyethersulfone (PES), with its high strength, high temperature resistance, corrosion- resistance, oxidation resistance and applicability under wide pH range, is used extensively as ultrafiltration and nanofiltration membrane. However, PES membranes foul easily in such an application because of hydrophobic nature of PES raw materials. Improving the hydrophilicity of PES by grafting hydrophilic monomers onto it is of potential to solve the problem. At present, common approaches to improve hydrophilicity of PES membranes are UV grafting modification, plasma modification, and chemical modification, whereas grafting and modifying PES films by 60Co γ-rays has rarely been reported. Studies have been carried out in our laboratory to graft hydrophilic monomers onto PES membranes directly or PES powders via simultaneous radiation grafting with the rays. Acrylic acid, methyl acrylic acid or acrylamide was used to study effects of the monomer concentration, irradiation dose and dose rate, solvent, inhibitor and pH of the grafting solution on the degree of grafting. The results showed that hydrophilicity of all the PES membranes could be improved, with the extent of improvement being dependent on the grafting conditions. (authors)

  12. Changes in membrane receptors of B and T human lymphocytes exposed to 60Co gamma rays

    International Nuclear Information System (INIS)

    The present report describes the effect of 50 to 2500 rad of 60Co gamma rays on the membrane receptors of B and T lymphocytes. Surface membrane immunoglobulins and Fc receptors were used as markers for B cells, while the formation of rosettes with sheep red blood cells was used as a marker for T cells. The effect of radiation is expressed as the percentage of viable cells that bear membrane receptors. The radiation effects were studied after 2, 24, 36 hr of culture. For B lymphocytes no reduction in SmIg and Fc receptors was seen 2 hr after radiation, whereas a dose-related reduction was observed after 24 and 36 hr. A dose-related inhibition of the ''capping'' of SmIg was observed. For T lymphocytes a reduction of E rosette formation was seen starting 2 hr after irradiation. Electron microscopy of separated B and T cells showed a higher sensitivity of T cells as well as a different structural arrangement of the chromatin as a consequence of radiation damage. These data suggest differentiated behavior of B and T lymphocyte membrane receptors after exposure to radiation

  13. Evaluation of 60Co radiation effect in the survival of different mouse strains. Radiomodifiers and celular response

    International Nuclear Information System (INIS)

    The radiomodifier capacity of proteose-peptone (PP), imidazole derivatives such as azomycin and levamisole against an 8 or 9 Gy single dose of 60Co irradiation of mice from IPEN animal house was evaluated, being the biological responses compared with other mouse strains. It is concluded that PP, azomycin and PP + azomycin behaved as radioprotectors, while lavamisole appeared as a radiossensitizer. The various strains showed differences in their survival indexes. The changes in body weight curves of mice from all the experiments were followed during 30 days. Qualitative and quantitative analysis 2 hours, 3 and 6 days after irradiation of typical macrophages, mononuclear cells (monocytes and lymphocytes), polimorphonuclear and mast cells from peritonium of test animals showed that radiation interfered in a differential way in the kinetics of peritoneal cells. (author)

  14. Biological dosimetry in radiological protection: dose response curves elaboration for 60Co and 137Cs

    International Nuclear Information System (INIS)

    Ionizing radiation sources for pacific uses are being extensively utilized by modern society and the applications of these sources have raised the probability of the occurrence of accidents. The accidental exposition to radiation creates a necessity of the development of methods to evaluate dose quantity. This data could be obtained by the measurement of damage caused by radiation in the exposed person. The radiation dose can be estimated in exposed persons through physical methods (physical dosimetry) but the biological methods can't be dispensed, and among them, the cytogenetic one that makes use of chromosome aberrations (dicentric and centric ring) formed in peripheral blood lymphocytes (PBL) exposed to ionizing radiation. This method correlates the frequency of radioinduced aberrations with the estimated absorbed dose, as in vitro as in vivo, which is called cytogenetic dosimetry. By the introduction of improved new techniques in culture, in the interpretation of aberrations in the different analysers of slides and by the adoption of different statistical programs to analyse the data, significant differences are observed among laboratories in dose-response curves (calibration curves). The estimation of absorbed dose utilizing other laboratory calibration curves may introduce some uncertainties, so the International Atomic Energy Agency (IAEA) advises that each laboratory elaborates your own dose-response curve for cytogenetic dosimetry. The results were obtained from peripheral blood lymphocytes of the healthy and no-smoking donors exposed to 60Co and 137Cs radiation, with dose rate of 5 cGy.min.-1. Six points of dose were determined 20,50,100,200,300,400 cGy and the control not irradiated. The analysed aberrations were of chromosomic type, dicentric and centric ring. The dose response curve for dicentrics were obtained by frequencies weighted in liner-quadratic mathematic model and the equation resulted were for 60Co: Y = (3 46 +- 2.14)10-4 cGy-1 + (3.45 +- 0

  15. Development and evaluation of a technique for in vivo monitoring of 60Co in human lungs

    Science.gov (United States)

    de Mello, J. Q.; Lucena, E. A.; Dantas, A. L. A.; Dantas, B. M.

    2016-07-01

    60Co is a fission product of 235U and represents a risk of internal exposure of workers in nuclear power plants, especially those involved in the maintenance of potentially contaminated parts and equipment. The control of 60Co intake by inhalation can be performed through in vivo monitoring. This work describes the evaluation of a technique through the minimum detectable activity and the corresponding minimum detectable effective doses, based on biokinetic and dosimetric models of 60Co in the human body. The results allow to state that the technique is suitable either for monitoring of occupational exposures or evaluation of accidental intake.

  16. Monte Carlo calculation of 60Co γ-ray's albedo-dose rate from the air

    International Nuclear Information System (INIS)

    The Monte Carlo calculation of 60Co γ-ray's albedo-dose rate from the air is reported. A formula is presented with which the relations of the albedo-doserate with some parameters are simulated and fitted

  17. Bioremediation of 60Co from simulated spent decontamination solutions of nuclear power reactors by bacteria

    International Nuclear Information System (INIS)

    The spent decontamination solutions generated from nuclear power reactors contain radionuclides of cobalt (60Co, 56Co and 57Co) along with a large excess (105) of non-radioactive metal ions (Fe, Ni and Cr). Our previous studies demonstrated that bioremediation of 60Co from simulated effluents using fungal biomass can provide an alternative to conventional ion exchangers. In this study, we used several bacteria to further improve the process of bioremediation by decreasing biomass requirement and treatment period. Further, metabolite activation in specific bacterial species resulted in enhanced bioremediation of 60Co from simulated effluent. Optimization of conditions in simulated effluent for the eight bacterial species to accomplish maximum 60Co removal is discussed. (author)

  18. Multistep process of neoplastic transformation of normal human fibroblasts by 60Co gamma rays and Harvey sarcoma viruses

    Energy Technology Data Exchange (ETDEWEB)

    Namba, M.; Nishitani, K.; Fukushima, F.; Kimoto, T.; Nose, K.

    1986-03-15

    As reported previously (Namba et al., 1985), normal human fibroblasts were transformed by 60Co gamma-ray irradiation into immortal cells with abnormal karyotypes. These transformed cells (KMST-6), however, showed a low cloning efficiency in soft agar and no transplantability. However, upon treatment with Harvey murine sarcoma virus (Ha-MSV), the cells acquired elevated clonability in soft agar and transplantability in nude mice. Ha-MSV alone, however, did not convert normal human fibroblasts into either immortal or tumorigenic cells. The Ha-MSV-transformed KMST-6 cells showed an enhanced expression of the ras oncogene, but normal and 60Co gamma-ray-transformed cells did not. Our current data suggest that gamma rays worked against normal human cells as an initiator, giving rise to chromosome aberrations and immortality, and that Ha-MSV, probably through its ras oncogene, played a role in the progression of the malignant cell population to a more malignant one showing enhanced colony formation in soft agar and tumorigenicity in nude mice.

  19. Study on the therapeutic effect of cytokine on acute radiation syndrome induced by 60Co γ ray exposure

    International Nuclear Information System (INIS)

    Objective: To examine the therapeutic effect of combined administration of rhIL-11 and rhG-CSF on acute radiation syndrome induced by 60Coγ ray exposure in beagles. Methods: Sixteen beagles were randomly divided into the control (n=5), symptomatic treatment group (n=5) and cytokine treatment group (n=6). All the beagles in the three groups were exposed to the whole-body irradiation of 60Co γ ray at a dose of 4.5 Gy. The animals in the second group accepted active symptomatic treatment. The animals in the cytokine treatment group were sub-cutaneously administered rhIL -11 and rhG -CSF besides active symptomatic treatment. Hemogram of peripheral blood, pristine apoptosis and necrosis ratio of nucleated cells, the content of IL-2 and IFN-γ in plasma and formation of bone marrow were used to evaluate the therapeutic effect. Results: In the cytokine treatment group, the animal' survival rate 45 days after exposure was increased and hemogram of peripheral blood was improved significantly. The rate of pristine apoptosis and necrosis of nucleated cell declined obviously. Early, the content of both IL-2 and IFN-γ in plasma was up significantly, then the content of IL-2 rapidly descended, but that of IFN-γ was relatively stable. Conclusion: The combined administration of rhIL-11 and rhG-CSF significantly improved the recovery of hematopoietic and immunological function. (authors)

  20. Intra-arterial administration of carboplatin plus lower dosage radiation of 60CO as induction treatment in advanced oral cancer

    International Nuclear Information System (INIS)

    Conventional pre-operative chemoradiotherapy often causes severe side effects, which may result in interruption of the treatment and delay of decided operation. Carboplatin (CBDCA) is one of the effective chemotherapeutants for head and neck cancer. We treated 23 patients with advanced oral cancers by a combination of intra-arterial administration of Carboplatin and 60CO radiotherapy. The dosage of Carboplatin was between 20 mg and 35 mg per square meter of body surface. The dosage of external 60CO irradiation was 2 Gy per day and 30 to 60 Gy in total. We evaluated clinical response, toxicity and survival of this therapy of all the patients. Histologic response was also evaluated in some of them. All cancers responded to the regional chemoradiotherapy and demonstrated remission. Two (8%) completed response rate (CR) and 16 (69%) partial response rate (PR) were achieved. The accumulated five-year overall survival rate by Kaplan-Meier method was 73.9%. Fourteen patients (60.8%) showed no evidence of disease (NED) within five years after the therapy. All patients had stomatitis, but most of them were not so severe. The major hematological toxicity was leukopenia, but it was from mild to moderate and reversible. Our study showed that this therapy provided low toxicity, high clinical and histological response rate. (author)

  1. Optimization of the cryopreservation of biological resources, Toxoplasma gondii tachyzoites, using flow cytometry.

    Science.gov (United States)

    Mzabi, Alexandre; Escotte-Binet, Sandie; Le Naour, Richard; Ortis, Naïma; Audonnet, Sandra; Dardé, Marie-Laure; Aubert, Dominique; Villena, Isabelle

    2015-12-01

    The conservation of Toxoplasma gondii strains isolated from humans and animals is essential for conducting studies on Toxoplasma. Conservation is the main function of the French Biological Toxoplasma Resource Centre (BRC Toxoplasma, France, http://www.toxocrb.com/). In this study, we have determined the suitability of a standard cryopreservation methodology for different Toxoplasma strains using the viability of tachyzoites assayed by flow cytometry with dual fluorescent labelling (calcein acetoxymethyl ester and propidium iodide) of tachyzoites. This method provides a comparative quantitative assessment of viability after thawing. The results helped to define and refine quality criteria before tachyzoite cryopreservation and optimization of the cryopreservation parameters. The optimized cryopreservation method uses a volume of 1.0 mL containing 8 × 10(6) tachyzoites, in Iscove's Modified Dulbecco's Medium (IMDM) containing 10% foetal calf serum (FCS). The cryoprotectant additive is 10% v/v Me2SO without incubation. A cooling rate of ∼1 °C/min to -80 °C followed, after 48 h, by storage in liquid nitrogen. Thawing was performed using a 37 °C water bath that produced a warming rate of ∼100 °C/min, and samples were then diluted 1:5 in IMDM with 5% FCS, and centrifuged and resuspended for viability assessment.

  2. 紫外和60Co-γ诱变对树干毕赤酵母发酵木糖产乙醇的影响%Effects of Mutagenesis by UV lrradiation and 60Co-γlrradiation on Fermentation of Xylose to Ethanol by Pichia stipitis

    Institute of Scientific and Technical Information of China (English)

    刘阳; 熊冬梅; 熊兴耀; 苏小军; 蔡柳; 曾璐

    2015-01-01

    ln this study, effects of UV irradiation and 60Coirradiation on fermenta-tion of xylose to ethanol by Pichia stipitis were analyzed to investigate the optimal mutagenic conditions. According to the growth curve and fermentation curve of P. stipi-tis, the optimal incubation duration and fermentation duration of P. stipitis mutant strain were 18 and 48 h, respectively. The cel concentration of original yeast liquid was 107 cel s/ml. After mutagenesis by UV irradiation and 60Coirradiation, yeast liquid was incubated in 20 g/L xylose media for 48 h. According to the results, after UV irradiation for 45-75 s, transformation efficiency reached 0.3794 g/g, which was 74.39% of the theoretical value; after irradiation with 800-1 000 Gy 60Co-γ, transforma-tion efficiency reached 0.3165 g/g, which was 62.06% of the theoretical value. Therefore, both UV irradiation and 60Coirradiation could improve the efficiency of xylose fermentation to ethanol by P. stipitis under appropriate conditions.%以树干毕赤酵母为出发菌株,研究紫外短波照射和60Co-γ辐照2种诱变方法对该菌种利用木糖产乙醇性能的影响,探讨较佳诱变条件。通过绘制菌体生长曲线及发酵曲线,确定了诱变菌最适宜的培养时长为18 h,诱变菌最适宜的发酵时长为48 h。原菌液浓度为107个/ml时,根据诱变后菌液在木糖浓度20 g/L中培养48h的结果可知,紫外处理45~75 s乙醇产率可达0.3794 g/g,为理论值的74.39%,辐照处理800~1000 Gy,乙醇产率可达0.3165 g/g,为理论值的62.06%。因此,紫外及辐照这2种诱变方式在合适的参数条件下均能提高树干毕赤酵母利用木糖产乙醇的转化率。

  3. Radiation-induced micronucleus formation in mice bone marrow after exposure to different doses of 60Co gamma radiation

    International Nuclear Information System (INIS)

    The frequency of micronuclei formation in the polychromatic erythrocytes and normochromatic erythrocytes was studied at 12 and 24th post-irradiation in mice bone marrow whole-body exposed to 0, 0.5, 1.0, 2.0, 3.0 and 4.0 Gy of 60Co gamma radiation. It was observed that the frequency of MPCE (micronucleated polychromatic erythrocytes) and MNCE (micronucleated normochromatic erythrocytes) increased with increase in exposure dose in both intervals studied. The data analyzed using the linear quadratic (Y+C+αD+βD2) equation. It was found that the data for MPCE and MNCE fitted best for linear quadratic model. (The PCE/NCE ratio declined with increase in exposure dose in both intervals and this decline was dose related. (author). 28 refs., 1 tab

  4. In vitro response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells exposed to {sup 60}Co at single fraction

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Lidia Maria; Campos, Tarcisio Passos Ribeiro de [Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil). Dept. de Engenharia Nuclear]. E-mail: lidia.andrade@unifenas.br; Leite, M.F. [Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil). Dept. de Fisiologia e Biofisica; Goes, A.M. [Universidade Federal de Minas Gerais, Belo Horizonte, MG (Brazil). Dept. de Bioquimica e Imunologia

    2005-10-15

    Radiotherapy using gamma rays is a common modality of breast cancer treatment. The aim of this research is to investigate the biological response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells (PBMC) exposed in vitro to {sup 60} Co irradiation at a single fraction of 10 Gy, 25 Gy and 50 Gy doses at 136,4 cGy.min{sup -1} rate. Cells were irradiated at room temperature by the Theratron 80 radiotherapy system. Biological response was evaluated through cellular viability using MTT assay and nucleus damages visualized by Propidium Iodide assay and electrophoresis agarose gel after gamma irradiation. Nucleus damages induced by {sup 60} Co irradiation were compared to damage caused by cell exposure to 10% methanol. The 50 Gy dose of irradiation did not stimulate nucleus damages at the same level as that affected by 10% methanol induction in the MDAMB-231. Further studies are necessary to understand these mechanisms in the MDAMB-231 human breast carcinoma cell line.(author)

  5. Dominant lethal effect of gamma radiation of 60Co in Biomphalaria glabrata (SAY, 1818)

    International Nuclear Information System (INIS)

    Germ cell mutations are used in ecotoxicological studies as biomarkers of population effects and indicators of ecological changes. Biomphalaria glabrata, a freshwater mollusk, is a good experimental model for biomonitoring studies due to its biological characteristics and the ecological importance of this invertebrate group. The dominant lethal test was established in B. glabrata for the detection of germ cell mutations. Results with chemical mutagens showed that this system is efficient, specific and sensitive in the evaluation of germ cell mutations induced by reference mutagens. In this work, the dominant lethal effects of gamma radiation of 60Co were studied. A preliminary experiment was done to establish the dose range and to estimate the chronology of spermatogenesis in B. glabrata. This estimate is possible because of the uniformity in response to ionizing radiation between germ cells at homologous stages of spermatogenesis in widely different species. In general, pre-meiotic germ cells are less sensitive to the induction of lethal dominant mutations than post-meiotic cells. This effect can be attributed to: young gametogenic cells - mitotically active - have greater repair ability from sub-lethal DNA damage and there is a selective elimination of the damaged cells. In our system: induction of lethal dominant mutations causes an increase in the frequency of malformations and, cytotoxic effect is displayed as a reduction in the crossing rates. Total duration of spermatogenesis was estimated in approximately 36 days, with the following distribution of stages: 1 to 13 days - spermatogonia, 14 to 20 days - spermatocytes, 21 to 36 days - spermatids and spermatozoa. Based on this chronology, irradiated wild-type snails with 2,5; 10 and 20Gy and crossed with non-irradiated albino snails after 7, 17, 23, 30 and 36 days. The frequencies of malformations in the heterozygous wild-type offspring of the nonirradiated albino snails were used as indicator of germ cell

  6. Comparative uptake from sea water and tissue distribution of 60Co in marine mollusks

    International Nuclear Information System (INIS)

    Five different species of marine mollusks, Mytilus galloprovincialis Lmk., Tapes decussatus L., Cerastoderma (Cardium) edule (L.), Donax vittatus (da Costa) and Patella vulgata L., were exposed to 60Co-labelled sea water under laboratory conditions. After a 1-mo exposure, tested species reached different whole-body 60Co concentration factors (CF) over radioactive sea water of 73 +/- 27, 22 +/- 10, 84 +/- 25, 6.3 +/- 1.4 and 31 +/- 10, respectively, which are not dependent upon the size of mollusks. Equations for the experimental uptake curves, obtained using a multi-exponential model, indicate that 60Co uptake by mollusks involves two or three compartments, according to the species. In all species, the larger compartments turn over with long biological half-lives, dependent upon species. At the beginning of the experiment, 60CoCl2 added to sea water was mainly in cationic forms. These forms were progressively converted into anionic plus neutral forms most likely due to complex formation with organic ligands. With time this physico-chemical evolution had a lowering effect on 60Co bioaccumulation by mollusks. Analysis of 60Co in tissues revealed that Donax shell and mantle do not accumulate the radionuclide in great quantities, generating the low whole-body concentration factor found. In contrast, shell and mantle from all other species displayed variable but high CFs. Shell by itself accounts for more than half of the 60Co whole-body burden. Among soft tissues, gills and viscera displayed the highest CF and muscle the lowest. From these experiments, one may conclude that significant differences among species do exist regarding Co bioaccumulation potential

  7. Comparative uptake from sea water and tissue distribution of 60Co in marine mollusks

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, F.P.

    1987-07-01

    Five different species of marine mollusks, Mytilus galloprovincialis Lmk., Tapes decussatus L., Cerastoderma (Cardium) edule (L.), Donax vittatus (da Costa) and Patella vulgata L., were exposed to /sup 60/Co-labelled sea water under laboratory conditions. After a 1-mo exposure, tested species reached different whole-body /sup 60/Co concentration factors (CF) over radioactive sea water of 73 +/- 27, 22 +/- 10, 84 +/- 25, 6.3 +/- 1.4 and 31 +/- 10, respectively, which are not dependent upon the size of mollusks. Equations for the experimental uptake curves, obtained using a multi-exponential model, indicate that /sup 60/Co uptake by mollusks involves two or three compartments, according to the species. In all species, the larger compartments turn over with long biological half-lives, dependent upon species. At the beginning of the experiment, /sup 60/CoCl2 added to sea water was mainly in cationic forms. These forms were progressively converted into anionic plus neutral forms most likely due to complex formation with organic ligands. With time this physico-chemical evolution had a lowering effect on /sup 60/Co bioaccumulation by mollusks. Analysis of /sup 60/Co in tissues revealed that Donax shell and mantle do not accumulate the radionuclide in great quantities, generating the low whole-body concentration factor found. In contrast, shell and mantle from all other species displayed variable but high CFs. Shell by itself accounts for more than half of the /sup 60/Co whole-body burden. Among soft tissues, gills and viscera displayed the highest CF and muscle the lowest. From these experiments, one may conclude that significant differences among species do exist regarding Co bioaccumulation potential.

  8. Mechanisms of60Co uptake and loss by Scenedesmus obliquus and transfer to two benthic organisms

    International Nuclear Information System (INIS)

    The affinity of Scenedesmus obliquus for 60Co has been confirmed under various experimental conditions allowing for the age of cultures, the physiological state of the cells, the radioactivity level and stable cobalt concentration in the medium, and the acute or periodic nature of the contamination. The dry weight 60Co concentration factor may reach 4x104 in LCO culture medium and is about 104 in natural water. 60Co uptake by algae is a 95% passive phenomenon, initiated by radionuclide absorption on the cell walls and followed by intracellular diffusion in accordance with Fick's law. From the initial cationic form, radiocobalt develops anion forms at a rate proportional to the algal biomass, resulting in lower contamination of old and dense cultures. After transfer in natural or artificial nonradioctive medium, 60Co is eliminated by Scenedesmus obliquus, mainly as Co2+ ions, according to a two-phase exponential process with two biological half-lives. The study of radioactive cobalt transfer from algae to gammarids and to midge larvae reveals the extend to which phytoplankton predominate over water and sediment in contaminating these two organisms. The total 60Co uptake depends significantly on whether the cells are incorporated in or deposited on the sediment. No biomagnification phenomenon of the radionuclide was found in any case, as the transfer factor was less than 1. Depuration of 60Co by organisms involved a two-phase exponential process regardless of whether uptake occured directly or from food. The presence of sediment appears to reduce the 60Co desorption rate, but from 85 to 95% of the initial quantity is eliminated. This percentage does not depend on the transfer pathway or the presence of organic matter, and indicates very slight assimilation of radiocobalt ingested with algal cells

  9. The effect of 60Co γ-rays on the contents of paramylon and trehalose in Euglena gracilis

    International Nuclear Information System (INIS)

    Full text: It is reported that the non-reducing disaccharide trehalose is accumulated in various organisms and protects biomolecules against various environmental stresses including heat shock, osmotic shock, and oxidative stresses. Euglena (Euglena gracilis) is photosynthetic unicellular organisms which is classified both as animal and as plant because of its motility. In our previous study, it was shown that salt stress causes degradation of paramylon, the reserve carbohydrate of Euglena, and trehalose, the degraded product of paramylon, accumulated simultaneously to maintain the osmotic pressure of Euglena cells. Euglena is known to be radiation-resistant more than Escherichia coli and other bacteria. Therefore, we have investigated whether or not the same phenomenon could be observed in Euglena after 60Co-gamma irradiation. Exposure of the native Z strain and chloroplast-deficient SM-ZK strain of Euglena to a dose of 500 kGy induced significant paramylon degradation and trehalose accumulation during the incubation in the Koren-Hunter culture medium containing glucose as carbon source after the irradiation. Forty percent of the paramylon was degraded within 10-hr incubation after the irradiation but accumulated trehalose content was about one sixth of that by salt stress. Trehlose accumulation disappeared when the irradiated cells were incubated in phosphate buffer but it recovered when glucose is added to the buffer to the same concentration as K-H media. These results suggest that trehalose accumulation in irradiated Euglena cells plays a role in protecting cellular constituents from oxidative damage caused by gamma-irradiation independently of the response to salt stress

  10. Monte Carlo dosimetry for 125I and 60Co in eye plaque therapy

    International Nuclear Information System (INIS)

    Monte Carlo calculations of radiation dosimetry using morse code are performed for 125I and 60Co point sources in a cylindrical head phantom that simulates the geometry of eye plaque therapy for choroidal melanoma. We obtain the dose variation in the eye at submillimeter intervals over distances as close as 1 mm and up to 2.5 cm from the source. The calculations for 125I are performed for the phantom media of water, protein, and a homogenized protein--water mixture simulating the composition of the eye. Relative dose functions for 125I for these phantom media are fitted to second-degree polynomials. Agreement is found with published results. The relative dose function for 60Co at eye position in the water head phantom is fitted to a third-degree polynomial and compared with that for 60Co at the center of a large water sphere. A boundary effect due to the head phantom--air interface on the dose distribution for 60Co is demonstrated. The dose falloff with distance is faster for the eye geometry compared with the bulk geometry. We also show that the relative dose distributions within the tumor are comparable for 125I and 60Co by comparing their relative dose functions. This result is consistent with the success of clinical trials of large melanoma treatments with 125I plaques

  11. The effects of 60Co γ-ray on poly(ethylene-co-vinyl acetate)/carbon black composites

    Science.gov (United States)

    Lee, Kyoung-Yong; Kim, Ki-Yup

    2008-04-01

    Cables used in a nuclear power plant are irradiation suppressing ones. Until now, researches on the irradiation suppressing cables have mainly been focused on insulation materials. Therefore, in this paper, the non-isothermal crystallization behaviors and degradation characteristics of ethylene vinyl acetate-carbon black (EVA-CB), used as a shielding material, were investigated by means of the Differential scanning calorimetry (DSC) and chemiluminescence analyzer (CL). The specimens were cooled after removing thermal history at 150 °C for 5 min by changing the cooling rates to 5, 7.5, 10, 15 and 20 °C/min with DSC. In addition, after maintaining a thermal equilibrium at each temperature of 25, 50, 75, 100, 125, 150 and 175 °C, their thermoluminescence was measured for 20 min with CL equipment. The 60Co γ-ray was used for irradiation. Tc, T0, T∞ and t1/2 in the DSC experiments are found to decrease gradually as radiation dose increases. Secondly, with the CL experiment, the 0.1, 0.25 and 0.5 MGy EVA-CB composites were found to show a much smaller thermoluminescence than the intact EVA-CB composites, while the 0.75 and 1 MGy EVA-CB composites were found to show a much higher thermoluminescence than ones.

  12. High dose rate sup 60 Co-RALS intraluminal radiation therapy for advanced biliary tract cancer with obstructive jaundice

    Energy Technology Data Exchange (ETDEWEB)

    Tamada, Toshiaki; Yoshimura, Hitoshi; Yoshioka, Tetsuya; Iwata, Kazuro; Ohishi, Hajime; Uchida, Hideo (Nara Medical Univ., Kashihara (Japan))

    1991-12-01

    High-dose-rate intraluminal irradiation with a {sup 60}Co remote afterloading system, using our newly developed applicator, was performed in 15 cases of advanced biliary tract cancer (2 gallbladder cancers, 13 cholangiocarcinomas). The applicator which was thin with an outer diameter of 14 Fr had a double lumen which included a small lumen for a guide wire for easy exchange to a drainage tube. Therefore, the irradiation procedure could be easily finished in a short time within little distress to the patient. The tumor marker CA1 19-9 was useful in assessing the therapeutic effect and follow-up observation after treatment. The average tube-free period and survival time were 5.9 months and 8.3 months, respectively. Histopathological examination of the cases autopsied showed that the combination of intraluminal (30 Gy) and external (30 Gy) irradiation offered good local control of the cancer that was within 1 cm from the source. Cholangiocarcinoma with relatively short length of obstruction, stage III or stage IV when the cancer infiltration was localized around the bile duct, achieved a comparatively long survival time. It was suggested that the combined use of high-dose-rate intraluminal radiotherapy, with precisely delivered radical radiation to tumors localized in the bile duct wall, and external radiotherapy delivered as additional radiation to the surrounding bile duct would provide radiotherapeutic advantage and contribute to survival after treatment for advanced biliary tract cancer. (author).

  13. Characterization of 60Co dose distribution using BEAMnrc Monte Carlo code

    International Nuclear Information System (INIS)

    In this study BEAMnrc based on EGSnrc as Monte Carlo code has been used for modeling and simulating 60Co machine in radioisotope centre of Khartoum (RICK), Two fields size ( 5 cm x 5 cm and 35 cm x 35 cm), were been studied, to define the characterization of 60Co machine and to investigate the effect of increasing the surface to skin distance (SSD) on the 60Co machine properties, e.g.; beam profile and percentage depth dose (Pdd). For the narrow field size there is a small change observed in the curves representing beam profile and the percentage depth dose when increasing the distance by 5 cm, for the wide fi ld size there relatively clear different in curves. The study results been compared with other previous studies and clear consistence observed. (Author)

  14. 60Co contamination in recycled steel resulting in elevated civilian radiation doses: causes and challenges.

    Science.gov (United States)

    Chang, W P; Chan, C C; Wang, J D

    1997-09-01

    Since late 1992, more than 100 building complexes containing public and private schools and nearly 1,000 apartments have been identified in Taiwan with elevated levels of gamma-radiation from construction steel contaminated with 60Co. Due to improper handling of 60Co contaminated scrap steel in late 1982 and 1983, contaminated construction materials have been widely distributed throughout the country. These contaminated construction materials have generated elevated radiation exposures to members of the public in Taiwan. As of early 1996, more than 4,000 people, including young students, have been identified as receiving more than 1 mSv y(-1) above the local background for up to 12 y. This report provides a detailed discussion of the sources of the 60Co contamination in construction steel, its discovery in the building complexes, and preliminary evaluation and remediation activities.

  15. Quality control and calibration of the ZEUS forward and rear calorimeters with 60Co sources

    International Nuclear Information System (INIS)

    We present the motivation for and the design of a mobile 60Co source system used as part of the quality control and calibration monitoring scheme for the ZEUS calorimeters. A 2 mCi 60Co source is pushed by a computer controlled drive mechanism through guide tubes which extend into the calorimeter. Measurements of induced photocurrents as a function of the source position allow checks on the calorimeter response. We present results obtained during the initial scan of all 1024 towers of the forward and rear calorimeter modules. (orig.)

  16. Validation of dose-response curve of CRCN-NE - Regional Center for Nuclear Sciences from Northeast Brazil for {sup 60}Co: preliminary results; Validacao da curva dose-resposta do CRCN-NE para {sup 60}Co: resultados preliminares

    Energy Technology Data Exchange (ETDEWEB)

    Mendonca, Julyanne C.G.; Mendes, Mariana E.; Hwang, Suy F.; Lima, Fabiana F. [Centro Regional de Ciencias Nucleares do Nordeste (CRCN-NE/CNEN-PE), Recife, PE (Brazil); Santos, Neide, E-mail: july_cgm@yahoo.com.br [Universidade Federal de Pernambuco (CCB/UFPE), Recife, PE (Brazil). Departamento de Genetica

    2014-07-01

    The cytogenetic study has the chromosomal alterations as biomarkers in absorbed dose estimation by the body of individuals involved in exposure to ionizing radiation by interpreting a dose response calibration curve. Since the development of the technique to the analysis of data, you can see protocol characteristics, leading the International Atomic Energy Agency indicate that any laboratory with intention to carry out biological dosimetry establish their own calibration curves. The Biological Dosimetry Laboratory of the Centro Regional de Ciencias Nucleares (CRCN-NE/CNEN), Brazil, recently established the calibration curve related to gamma radiation ({sup 60}Co). Thus, this work aimed to start the validation of this calibration curve from samples of three different blood donors which were irradiated with an absorbed known single dose of 1 Gy. Samples were exposed to {sup 60}Co source (Glaucoma 220) located in the Department of Nuclear Energy (DEN/UFPE). After fixation with methanol and acetic acid and 5% Giemsa staining, the frequency of chromosomal alterations (dicentric chromosomes, acentric rings and fragments) were established from reading of 500 metaphases per sample and doses were estimated using Dose Estimate program. The results showed that, using the dose-response curve calibration for dicentrics, the dose absorbed estimated for the three individuals ranged from 0.891 - 1,089Gy, taking into account the range of confidence of 95%. By using the dose-response curve for dicentrics added to rings and for the same interval of confidence the doses ranged from 0,849 - 1,081Gy. Thus, the estimative encompassed known absorbed dose the three individuals in confidence interval of 95%. These preliminary results seems to demonstrate that dicentric dose-response curves and dicentrics plus rings established by CRCN-NE / CNEN are valid for dose estimation in exposed individuals. This validation will continue with samples from different individuals at different doses.

  17. Study of recombination processes for 'electron-hole' pairs in germanium irradiated by {gamma} rays from {sup 60}Co using the photovoltaic effect in P-N junctions; Etude du processus de recombinaison des paires ''electron-trou'' dans le germanium irradie par les rayons {gamma} du cobalt 60 a l'aide de l'effet photovoltaique dans les jonctions P-N

    Energy Technology Data Exchange (ETDEWEB)

    Zahedi-Mochadam, A.A. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-10-01

    Using the photo-voltaic effect in p-n junctions, we have studied, during bombardment, the mechanism of the recombination of 'electron-hole' pairs in the presence of structure defects produced in germanium of the N and P types by {gamma} rays from a Co{sup 60} source. At 310 K the level of the recombination centres is situated 0.25 eV above the conduction band and the capture cross-sections of the holes and of the electrons have the respective values of: {sigma}{sub p} = 4 X 10{sup -15} cm{sup 2} and {sigma}{sub n} = 3 X 10{sup -15} cm{sup 2}. The value of {sigma}{sub n} appears to be under-estimated because the number of defects in P-type samples appears to be lower than that in N-type samples. These results lead to the conclusion that the interstitials are responsible for the recombination. At 80 K it has been found that in N-type samples, a shallow level exists at O.05 eV below the conduction band with a capture cross-section for the holes of {sigma}{sub p} {>=} 10{sup -14} cm{sup 2}. We believe that in this case the recombination of charge carriers is controlled by the neighbouring 'defect-interstitial' pairs. In P-type samples at low temperature, the life-time is practically constant during irradiation. This fact is attributed to a spontaneous annealing of defects ol purely electrical origin. In the last part of the work the study of the photo-voltaic effect applied to the problem of gamma radiation dosimetry is considered. It is shown that such dosimeters, based on this principle, make it possible to measure the intensity of gamma rays over a very wide range. (author) [French] En utilisant l'effet photovoltaique dans les jonctions p-n, nous avons etudie au cours du bombardement le mecanisme de recombinaison des paires 'electron-trou' en presence des defauts de structure introduits dans le germanium de type N et de type P par les rayons gamma d'une source de Co{sup 60}. A 310 K, le niveau des centres de recombinaison se

  18. In vivo effect of some home spices extracts on the Toxoplasma gondii Tachyzoites

    Directory of Open Access Journals (Sweden)

    Najia A Al-Zanbagi

    2009-01-01

    Full Text Available Toxoplasmosis drugs have the longest history and are still the first choice for most conditions. Alternative drugs such as Co-trimoxazole and Tetracycline have been tried and acclaimed successful. The lack of general acceptance, however, is an indication that the results are not very convincing. A wide range of antibiotics is urgently needed for patients with drug reaction or resistance problems. The anti-toxoplasmic activity of water and ethanol extracts as well as the oil of some home spices (Piper nigrum, Capsicum frutescens, Cinnamomum cassia and Curcuma longa, were evaluated in murine models of intraperitoneal infection using the RH strain of Toxoplasma gondii. Female mice were infected with 2x10 [2] tachyzoites/ml, and then treated intraperitoneally with the home spices at 100 and 200 mg/kg/day for seven days. The tested extracts reduced the mean number of tachyzoites present in the peritoneal fluid of the experimental mice. The most effective extract was Curcuma longa ethanol extract which showed a 98.6% and 99.2% inhibition of the growth of Toxoplasma tachyzoites in 100 and 200 doses respectively compared to the control infected untreated mice.

  19. Use of {sup 60}Co gamma radiation in increased levels of total polyphenol extracts of bark of Schinus terebinthifolius Raddi

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Gustavo H.F.; Silva, Edvane B.; Silva, Hianna A.M.F.; Amorin, Elba L.C.; Peixoto, Tadeu J.S.; Yara, Ricardo; Lima, Claudia S.A., E-mail: santosghf@hotmail.com, E-mail: edvborges@yahoo.com, E-mail: amdemelo@hotmail.com, E-mail: claudia.salima@gmail.com, E-mail: ricardo.yara@gmail.com, E-mail: tadeu1903@yahoo.com.br, E-mail: elba@ufpe.br [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil)

    2013-07-01

    Schinus terebinthifolius Raddi (Anacardiaceae) is well known as sources of phenolic compounds. Known as mastic pepper, red pepper tree is a plant native to midsize coast of Brazil. Some of its structures have proven antibacterial, anti-inflammatory, antifungal and healing. The aim of this study was to evaluate the difference in the phenol contents of crude extracts that were measured after irradiating the barks of S. terebinthifolius using gamma radiation from {sup 60}Co. The crude extract were divided into a control group and eight experimental groups, which were separated based on the doses of gamma radiation to which they were exposed: 2.5, 5.0, 7.5, 10.0, 12.5, 15.0, 20.0 and 50.0 kGy (Assays were performed in triplicate). The results allow observe that gamma radiation promoted in extracts of bark of S. terebinthifolius, many percents increase (p> 0.05) of total polyphenol content between 2.5 kGy (41.93%) and 50.0 kGy (44.52%) compared to 0 kGy (30.07%), with the same gradual to 10.0 kGy, and reaching peak maximum at 10.0 kGy (68.44%). However, the study puts the process of gamma radiation from {sup 60}Co as an alternative significant increase in the percentage of some natural substances of plant material, and subsequently contribute to the augmentation of various therapeutic applications to which they are assigned. (author)

  20. The Application of Fucus vesiculosus as a Bioindicator of 60Co Concentrations in the Danish Straits

    DEFF Research Database (Denmark)

    Boelskifte, S.

    1985-01-01

    /or the UK; the Kattegat, where the sources are the Swedish nuclear power plants Ringhals and Barsebäck; and the Sound, where the initial mixing of the release from Barsebäck takes place. A power function can be estimated for the Kattegat and the Sound describing the content of 60Co in Fucus as a function...

  1. Clinical data from one year follow-up of victims of the radiation accident with 60Co in Bulgaria.

    Science.gov (United States)

    Djounova, J; Guleva, I; Negoicheva, K; Mileva, I; Panova, D; Rupova, I

    2014-09-01

    A severe radiation accident occurred on 14 June 2011 in an industrial irradiation facility for medical equipment sterilization in Bulgaria. Five people were exposed for 5-10 min to a 60Co source containing 137 TBq. The Emergency Department of the National Centre of Radiobiology and Radiation Protection (NCRRP), Bulgaria, put into practice the plans for providing medical care in radiation accidents and the procedures developed for assessment of injury severity, the decision-making algorithm regarding subsequent treatment, and the therapy for persons affected. The activities performed for initial assessment of the severity of injury of irradiated patients were published in 2012. Based on predictive assessments of the severity of radiation damage, it was decided that the victims required hospitalization at a specialized hematology clinic. Percy Hospital in Paris was chosen for this purpose. The aim of this report is to present the results of 1-y follow-up for three of the victims. Sadly, 1 mo after the accident, Patient 4 died from a heart attack. The medical opinion was that this was not a direct outcome of the irradiation. Patient 5 was only followed up for 4 mo (118 d) because medical follow-up is voluntary, and despite repeated calls, the patient did not respond. Medical examinations by a physician as well as hematology and biochemical tests were performed using standard laboratory methods. The obtained results were compared to the victims' personal reference limits obtained from annual health monitoring. After the accident, the recovery to normal content of peripheral blood cells was observed in all victims. Nevertheless, there were observed cases of thrombocytopenia, granulocytopenia, and leucocytopenia at various times after exposure. During the period of observation, morphological changes in red blood cells such as anisomicrocitosis, macrocytes, megalocytes, and polychromatic erythrocytes were demonstrated. During the 1-y observation period, all victims showed

  2. Monte Carlo Dosimetry of the 60Co BEBIG High Dose Rate for Brachytherapy.

    Directory of Open Access Journals (Sweden)

    Luciana Tourinho Campos

    Full Text Available The use of high-dose-rate brachytherapy is currently a widespread practice worldwide. The most common isotope source is 192Ir, but 60Co is also becoming available for HDR. One of main advantages of 60Co compared to 192Ir is the economic and practical benefit because of its longer half-live, which is 5.27 years. Recently, Eckert & Ziegler BEBIG, Germany, introduced a new afterloading brachytherapy machine (MultiSource®; it has the option to use either the 60Co or 192Ir HDR source. The source for the Monte Carlo calculations is the new 60Co source (model Co0.A86, which is referred to as the new BEBIG 60Co HDR source and is a modified version of the 60Co source (model GK60M21, which is also from BEBIG.The purpose of this work is to obtain the dosimetry parameters in accordance with the AAPM TG-43U1 formalism with Monte Carlo calculations regarding the BEBIG 60Co high-dose-rate brachytherapy to investigate the required treatment-planning parameters. The geometric design and material details of the source was provided by the manufacturer and was used to define the Monte Carlo geometry. To validate the source geometry, a few dosimetry parameters had to be calculated according to the AAPM TG-43U1 formalism. The dosimetry studies included the calculation of the air kerma strength Sk, collision kerma in water along the transverse axis with an unbounded phantom, dose rate constant and radial dose function. The Monte Carlo code system that was used was EGSnrc with a new cavity code, which is a part of EGS++ that allows calculating the radial dose function around the source. The spectrum to simulate 60Co was composed of two photon energies, 1.17 and 1.33 MeV. Only the gamma part of the spectrum was used; the contribution of the electrons to the dose is negligible because of the full absorption by the stainless-steel wall around the metallic 60Co. The XCOM photon cross-section library was used in subsequent simulations, and the photoelectric effect, pair

  3. Resposta de sementes de amendoim a diferentes doses de radiação gama (60Co Response of peanut seeds to different levels of gamma radiation (60Co

    Directory of Open Access Journals (Sweden)

    Tatiana da S. Santos

    2010-10-01

    Full Text Available A radiação gama, proveniente da fonte de 60Co, é bastante utilizada para esterilização, visando a prevenção da decomposição e a toxidez de origem microbiana em diversos produtos. O grau de radiossensibilidade de um embrião vegetal depende da espécie, do estágio de seu desenvolvimento durante a radiação, da dose empregada e do critério usado para medir o efeito biológico, sendo comumente utilizado o teste de germinação. Objetivou-se, com este trabalho, estudar a sensibilidade do amendoim a radiação gama e seus efeitos na germinação, no vigor e na micoflora das sementes da cultivar BRS Havana, irradiadas com uma fonte de 60Co, tipo gammacell, com taxa de dosagem de 12,5 kGy h-1. As doses testadas em kGy foram as seguintes: 0; 0,5; 1,0; 1,5; 2,0; 2,5; 3,0; 6,0; 9,0; 12,0; 15,0; 18,0; 21,0 e 24,0. Com os resultados obtidos, concluiu-se que as doses acima de 3,0 kGy prejudicaram a viabilidade das sementes e as doses acima de 12 kGy comprometeram totalmente o vigor e a germinação das sementes de amendoim. A radiação a partir da dose 2,0 kGy eliminou os fungos Aspergillus flavus e Aspergillus niger e, a partir da dose 3,0 kGy, eliminou o Aspergillus glaucus. O Penicilium spp. permaneceu em mais de 30% das sementes em todos os tratamentos com radiação, não sendo eliminado até a dose de 24 kGy.The gamma radiation from 60Co source is widely used for sterilization aiming at preventing decomposition and toxicity from microbes in several products. The degree of radiosensitvity of a plant embryo depends on the species, the development stage during radiation, doses used and the criteria used to measure the biological effect, the germination test, being commonly used.This work aimed to study the peanut sensitivity to gamma radiation and its effects in the germination, in the vigour and seeds microflora of cultivar BRS Havana, irradiated with 60Co source, type gammacell with rate of dosage of 12.5 kGy h-1. The tested doses were the

  4. Expression changes of human mitochondrial COX genes in human lymphocytes after exposed by 60Co γ-rays

    International Nuclear Information System (INIS)

    Objective: To explore the changes of human mitochondrial COX Ⅰ , COX Ⅱ and COX Ⅲ genes expression induced by ionizing irradiation. Methods: Changes of human COX genes expression were detected by RT-PCR and Real-time PCR 8 h after the irradiation in human lymphoblastoid cell lines,which were exposed to 1-10 Gy 60Co γ-rays. The protein levels were detected by flow cytometry and the COX activity was measured by colorimetry. The dose-effect relationships between the expression changes of the genes and the doses were established. The changes of these genes expression were also analyzed at different post-radiation time-points between 0.5 h and 72 h after irradiation of 5 Gy in order to explore the time-effect. Results: The expression of 3 genes at mRNA level was up-regulated. A good dose-effect relationship was showed for COXⅠ and COX Ⅲ at dose range of 0-3 Gy and 0-8 Gy for COX Ⅱ (FCOXⅠ=116. 62, FCOXⅡ=17. 89, FCOXⅢ = 8.20, P<0.05). For the time-effect after irradiation, the gene expression levels of COX Ⅱ and COX Ⅲ genes were up-regulated and the peak change occurred at 4 h after irradiation. For COX Ⅰ gene, the mRNA expression levels were down-regulated during 0.5-72 h (FCOXⅠ=31.99, FCOXⅡ=19.47, FCOXⅢ=20.64, P<0.05 ). At the protein level, the levels of COX Ⅰ and COX Ⅱ were lowered in lower doses and enhanced in higher doses, and the levels of COX Ⅲ were decreased at all dose levels (FCOXⅠ=16.96, FCOXⅡ=32.5, FCOXⅢ=6.51, P<0.05). The protein levels of COX Ⅰ and COX Ⅱ were enhanced during 4-72 h and 8-72 h respectively after 5 Gy irradiation (FCOXⅠ=14.68,FCOXⅡ=17.18, FCOXⅢ =2.52, P<0.05). The activities of COX were lowered at different dose levels and different time-points. Conclusions: Ionizing radiation might induce the changes in mitochondrial COX Ⅰ, COX Ⅱ and COX Ⅲ gene expression, and lead to the reduction of the COX activities. (authors)

  5. Long-term clearance of accidentally inhaled 60Co aerosols in humans

    International Nuclear Information System (INIS)

    Long-term body retention was measured on six workers inhaling 60Co aerosols incidentally during manipulation with a high activity 60Co source. An improved whole body counter technique and calibration provided good conditions to follow the body clearance over 4 years. A two-detector profile scanning arrangement was used to measure the activity distribution 'in vivo' over the lung region. The observed whole body retention followed a two-exponential time function between 10-1500 days. The consistency of the measured retention pattern with ICRP inhalation model was investigated assuming different aerosol size distributions. A possible extension of ICRP lung model is proposed to take into account the actual pulmonary lung clearance determined individually. (R.P.) 29 refs.; 15 figs.; 6 tabs

  6. Effects of {gamma} ({sup 60}Co) and {beta} ({sup 90}Sr) radiations in Chinese hamster ovarian cells (CHO-K1): induction of micronuclei and cell death; Efeitos das radiacoes {gamma} ({sup 60}Co) e {beta} ({sup 90}Sr) em celulas de ovario de hamster chines (CHO-K1): inducao de micronucleos e morte celular

    Energy Technology Data Exchange (ETDEWEB)

    Murakami, Daniella

    2003-07-01

    Among various types of ionising radiation, the beta emitter radionuclides are involved in many sectors of human activity, such as nuclear medicine, nuclear industries and biomedicine, with a consequent increased risk of accidental, occupational or therapeutic exposure. Despite their recognized importance, there is little information about the effect of beta particles at the cellular level when compared to other types of ionizing radiation. Thus, the objective of the present study was to evaluate the genotoxic and cytotoxic effects of {sup 90}Sr, a pure, highly energetic beta source, on CHO-K1 cells and to compare them with data obtained with {sup 60}Co. CHO-K1 cells irradiated with different doses (1.0, 2.5, 5.0, 7.5 Gy) of {sup 60}Co (0.34 Gy.min{sup -1}) and {sup 90}Sr (0.23 Gy.min{sup -1}) were processed for analysis of clonogenic death, induction of micronuclei (MN) and necrotic and apoptotic death. The survival curves obtained for both types of radiation were better fitted by the linear-quadratic model and were similar. However, the cytogenetic results showed that both the proportion of micronucleated cells and the magnitude of radioinduced lesions demonstrated by the analysis of MN distribution were significantly higher in cells irradiated with {sup 60}Co than in cells irradiated with {sup 90}Sr, whereas {sup 90}Sr was more damaging than {sup 60}Co in terms of cell death induction. Necrosis was the major type of death observed in CHO-K1 cells. The data obtained suggest that the low incidence of micronucleated cells after exposure to {sup 90}Sr may be a consequence of selective elimination of severely damaged cells from the population by the necrotic process at a higher rate than observed with {sup 60}Co exposure. The data obtained also demonstrated the need to use several parameters for a better estimate of cellular sensitivity to the action of genotoxic agents, which would be important in terms of radiobiology, oncology and therapeutics. (author)

  7. Experimental and Monte Carlo evaluation of an ionization chamber in a 60Co beam

    Science.gov (United States)

    Perini, A. P.; Neves, L. P.; Santos, W. S.; Caldas, L. V. E.

    2016-07-01

    Recently a special parallel-plate ionization chamber was developed and characterized at the Instituto de Pesquisas Energeticas e Nucleares. The operational tests presented results within the recommended limits. In order to determine the influence of some components of the ionization chamber on its response, Monte Carlo simulations were carried out. The experimental and simulation results pointed out that the dosimeter evaluated in the present work has favorable properties to be applied to 60Co dosimetry at calibration laboratories.

  8. Experimental and Monte Carlo evaluation of an ionization chamber in a {sup 60}Co beam

    Energy Technology Data Exchange (ETDEWEB)

    Perini, Ana P.; Neves, Lucio Pereira, E-mail: anapaula.perini@ufu.br [Universidade Federal de Uberlandia (INFIS/UFU), MG (Brazil). Instituto de Fisica; Santos, William S.; Caldas, Linda V.E. [Instituto de Pesquisas Energeticas e Nucleres (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2015-07-01

    Recently a special parallel-plate ionization chamber was developed and characterized at the Instituto de Pesquisas Energeticas e Nucleares. The operational tests presented results within the recommended limits. In order to determine the influence of some components of the ionization chamber on its response, Monte Carlo simulations were carried out. The experimental and simulation results pointed out that the dosimeter evaluated in the present work has favorable properties to be applied to {sup 60}Co dosimetry at calibration laboratories. (author)

  9. Immobilization of 60Co and 90Sr ions using red mud from aluminum industry

    OpenAIRE

    Milenković Aleksandra S.; Smičiklas Ivana D.; Marković Jelena P.; Vukelić Nikola S.

    2014-01-01

    The removal of 60Co and 90Sr from the aqueous phase was tested using red mud - the fine grained residue from bauxite ore processing. This industrial waste represents a mixture of numerous minerals, mainly oxides and hydroxides of Fe, Al, Si, and Ti. Experiments were conducted as a function of contact time, pH, and pollutant concentrations. Kinetic data were well fitted with a pseudo-second order equation. The calculated rate constants and initial sorption r...

  10. Evaluation of fruit productivity and quality in Hass avocado submitted to {sup 60}Co gamma radiation; Evaluacion de productividad y calidad de fruta en aguacate Hass sometido a radiacion gamma de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Cruz T, E. De la; Ibanez P, J.; Mijares O, P.; Garcia A, J.M. [Instituto nacional de Investigaciones Nucleares, Departamento de Biologia, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1999-07-01

    Evaluation of productivity, postharvest behavior and fruit quality was performed on four years Hass avocado trees irradiated with {sup 60} Co gamma rays in doses of 5, 10, 15, 20 and 25 Gy, established in the ''La Labor'' Experimental Center of the Centro de Investigaciones Cientificas y Tecnologias del Aguacate en el Estado de Mexico (CICTAMEX) at Temascaltepec Mexico. Productivity had a significant increase in the dose of 15 Gy being the average number of fruits nearly 400 % more than the control at fruit setting, being such difference reduced at fruit harvesting to 300 %. In regard to postharvest performance, the respiration index (mg CO{sub 2}/kg/hr) did not show significant differences among treatments. Also others variables such as physiological weight losses, texture, maturity pattern, and sensorial tests (color, flavor, aroma, texture) were not different in regard to the control. This mean that radiation has altered productivity but not the quality and postharvest behavior of fruits. (Author)

  11. Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii.

    Directory of Open Access Journals (Sweden)

    Michael S Behnke

    Full Text Available BACKGROUND: Apicomplexan parasites replicate by varied and unusual processes where the typically eukaryotic expansion of cellular components and chromosome cycle are coordinated with the biosynthesis of parasite-specific structures essential for transmission. METHODOLOGY/PRINCIPAL FINDINGS: Here we describe the global cell cycle transcriptome of the tachyzoite stage of Toxoplasma gondii. In dividing tachyzoites, more than a third of the mRNAs exhibit significant cyclical profiles whose timing correlates with biosynthetic events that unfold during daughter parasite formation. These 2,833 mRNAs have a bimodal organization with peak expression occurring in one of two transcriptional waves that are bounded by the transition into S phase and cell cycle exit following cytokinesis. The G1-subtranscriptome is enriched for genes required for basal biosynthetic and metabolic functions, similar to most eukaryotes, while the S/M-subtranscriptome is characterized by the uniquely apicomplexan requirements of parasite maturation, development of specialized organelles, and egress of infectious daughter cells. Two dozen AP2 transcription factors form a series through the tachyzoite cycle with successive sharp peaks of protein expression in the same timeframes as their mRNA patterns, indicating that the mechanisms responsible for the timing of protein delivery might be mediated by AP2 domains with different promoter recognition specificities. CONCLUSION/SIGNIFICANCE: Underlying each of the major events in apicomplexan cell cycles, and many more subordinate actions, are dynamic changes in parasite gene expression. The mechanisms responsible for cyclical gene expression timing are likely crucial to the efficiency of parasite replication and may provide new avenues for interfering with parasite growth.

  12. Transfer and mobility of 137 Cs and 60 Co in oxisol and alfisol

    International Nuclear Information System (INIS)

    One manner to assess potential mobility of radionuclides in soils is the use of sequential extraction procedures. These procedures intend to provide the radionuclide partitioning in geochemical phases of soil affected by changes in physico-chemical conditions. In this study a new sequential chemical extraction protocol was choose to evaluate 60 Co and 137 Cs mobility under a large range of physico-chemical soil properties. The results of sequential procedure was further compared with soil to plant transfer factors data for maize and radish and with soil properties, showing rather consistent results. The 137 Cs distribution in soil showed that Fe oxides are the main sink for this element and after 14 years after contamination the 137 Cs was still available for plants. The 60 Co distribution showed that Mn oxides are the main sink for this element in Alfisol and 5 years after contamination no 60 Co was detected as bioavailable neither detectable in maize. The knowledge of the bio-geochemical behavior of radionuclides in soil system can be useful for risk assessment studies, to be applied in the case of nuclear accident or contamination scenarios. (author)

  13. Development and characterization of a new graphite ionization chamber for dosimetry of {sup 60}Co beams

    Energy Technology Data Exchange (ETDEWEB)

    Neves, Lucio Pereira; Perini, Ana Paula; Santos, William de Souza; Caldas, Linda V.E., E-mail: lpneves@ipen.br, E-mail: aperini@ipen.br, E-mail: wssantos@ipen.br, E-mail: lcaldas@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2014-07-01

    Ionization chambers are the most employed dosimeters for precise measurements, as those required in radiotherapy. In this work, a new graphite ionization chamber was developed and characterized in order to compose a primary standard system for the beam dosimetry of the {sup 60}Co sources. This dosimeter is a cylindrical type ionization chamber, with walls and collecting electrode made of high-purity graphite, and the insulators and stem made of Teflon®. The walls are 3.0 mm thick, and it has a sensitive volume of 1.40 cm{sup 3}. The characterization was divided in two steps: experimental and Monte Carlo evaluations. This new dosimeter was evaluated in relation to its saturation curve, ion collection efficiency, polarity effect, short- and medium-term stabilities, leakage current, stabilization time, linearity of response and angular dependence. All results presented values within the established limits. The second part of the characterization process involved the determination of the correction factors, obtained by Monte Carlo simulations. Comparing these correction factors values with those from other primary standard laboratories, the highest differences were those for the wall and stem correction factors. The air-kerma rate of the {sup 60}Co source was determined with this new dosimeter and with the IPEN standard system, presenting a difference of 1.7%. These results indicate that this new dosimeter may be used as a primary standard system for {sup 60}Co gamma beams. (author)

  14. Development and characterization of a new graphite ionization chamber for dosimetry of 60Co beams

    International Nuclear Information System (INIS)

    Ionization chambers are the most employed dosimeters for precise measurements, as those required in radiotherapy. In this work, a new graphite ionization chamber was developed and characterized in order to compose a primary standard system for the beam dosimetry of the 60Co sources. This dosimeter is a cylindrical type ionization chamber, with walls and collecting electrode made of high-purity graphite, and the insulators and stem made of Teflon®. The walls are 3.0 mm thick, and it has a sensitive volume of 1.40 cm3. The characterization was divided in two steps: experimental and Monte Carlo evaluations. This new dosimeter was evaluated in relation to its saturation curve, ion collection efficiency, polarity effect, short- and medium-term stabilities, leakage current, stabilization time, linearity of response and angular dependence. All results presented values within the established limits. The second part of the characterization process involved the determination of the correction factors, obtained by Monte Carlo simulations. Comparing these correction factors values with those from other primary standard laboratories, the highest differences were those for the wall and stem correction factors. The air-kerma rate of the 60Co source was determined with this new dosimeter and with the IPEN standard system, presenting a difference of 1.7%. These results indicate that this new dosimeter may be used as a primary standard system for 60Co gamma beams. (author)

  15. Toxoplasma gondii: effects of {sup 60} Co ionizing radiation in the viability and infectivity, detected in vitro in LLC-MK2 cells and in vivo in C57BL/6J mice

    Energy Technology Data Exchange (ETDEWEB)

    Hiramoto, Roberto M.; Almeida, Beatriz S.V.; Cardoso, Roselaine P.A.; Andrade Junior, Heitor F. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)

    1997-12-01

    Toxoplasmosis, caused by Toxoplasma gondii, promotes devasting disease in fetus and AIDS patients. The longlife immunity of natural infection is inefficient in eliminate tissue infective cysts. Few immunization programs were tested, mostly with attenuated strains. Ionizing radiation were used with successful in vaccine production, without reproductive ability with a relatively normal physiology until reproduction. Here, we tested several schedules of {sup 60} Co irradiation of tachzoites from RH strain of T. gondii, from peritoneal exudate or suspensions of LLC-MK2 infected cells, to optimize the viability and sterility of the irradiated agents. The tachzoites were exposed to 50, 100 and 200 Gy in a GammaCell 220 at 366 Gy/h. The viability was tested by motility, integrity and Trypan Blue dye exclusion. All irradiation schedules maintained a high (>90%) viability of the parasites. Dilutions were injected in C57Bl/6j mice with induction of specific antibodies, no clinical disease but uncertain sterility. Infection of LLC--MK2 cells showed that viable and reproductive parasites were often found in 50 Gy irradiated cells, rarely found in 100 Gy irradiated cells, rarely found in 100 Gy irradied cells, with no growth occuring with 200 Gy irradiated tachzoites. Our data show that 200 Gy {sup 60} Co irradiation blocks the reproductive capacity without affecting the short term viability of tachzoites of T. gondii. (author). 11 refs., 1 fig.

  16. 60 Co-γ辐射对白刺花幼苗生理的影响%Effect of 60 Co-γ lrradiation on Physiological Characteristics of Sophora viciifoliaSeedlings

    Institute of Scientific and Technical Information of China (English)

    李凤涛; 赵丽丽; 王普昶; 陈超; 吴小丽

    2015-01-01

    The relative physiological indexes of seedlings from S. viciifolia seeds irradiated by different dosage of 60 Co-γ treatment and the mutation effect of S. viciifolia seedlings was comprehensively evaluated by principal component analysis(PCA)and membership function analysis to provide the theoretical basis for breeding of S.viciifolia variety. The results show that chlorophyll,MDA,soluble sugar and soluble protein content and POD activity of S. viciifolia seedlings both represent a first rising and then decline trend with increase of irradiation dosage but the SOD activity and proline content gradually increase with increase of irradiation dosage. The different irradiation treatment can improve photosynthesis of S. viciifolia seedlings. MDA content of seedlings of S. viciifolia seeds treated with 60 kR is up to 59. 42 μmol/ g,significantly higher than other treatments. The POD and SOD activity of seedlings of S. viciifolia seeds treated with 100 kR and 120 kR reaches 596. 92 U/(g·min)and 178. 04 U/ g FW respectively. The soluble sugar and soluble protein content of seedlings of S. viciifolia seeds treated with 100 kR is the maximum. The proline content of seedlings of S. viciifolia seeds treated with 120 kR is seedlings’ physiological indexes affected by irradiation dosage is 120 kR ﹥ 100 kR ﹥ 80 kR ﹥ 140 kR ﹥ 60 kR ﹥ 40 kR ﹥ 20 kR﹥0 kR and the optimum irradiation dosage for S. viciifolia seeds is 80 kR,100 kR and 120 kR.%为白刺花新品种的培育提供理论依据,通过设置不同辐照剂量处理,以经辐照处理的白刺花种子培养的幼苗为研究对象,测定60 Co-γ辐射白刺花幼苗的相关生理指标,采用主成分分析和隶属函数分析法综合评价60 Co-γ射线对白刺花幼苗的诱变效果。结果表明:随着辐射剂量的增加,白刺花的叶绿素、MDA、可溶性糖和可溶性蛋白含量以及 POD 活性呈先升高后降低变化趋势,SOD 活性和脯氨酸含量则随着辐射

  17. Behavior of the sorption of {sup 60} Co in aqueous solution on inorganic materials as function of p H; Comportamiento de la sorcion del {sup 60} Co en solucion acuosa sobre materiales inorganicos como una funcion del pH

    Energy Technology Data Exchange (ETDEWEB)

    Granados, F.; Bulbulian, S.; Solache R, M. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico); Bertin, V. [UAM-I, 09340 Mexico D.F. (Mexico)

    2004-07-01

    The sorption of the {sup 60} Co is evaluated in aqueous solution on Mg O, MnO{sub 2}, TiO{sub 2}, Sn O, activated carbon and hydrotalcite calcined as a function of the p H, using the method for lots and quantifying at the {sup 60} Co for gamma spectrometry. Likewise it was explained the one behavior of the sorption of the {sup 60} Co in the materials with base in the chemical species of this radioactive isotope in aqueous solution. The chemical species of the {sup 60} Co in solution were identified by electrophoresis of high voltage for the different p H values. It was found that under the experimental conditions, the {sup 60} Co showed a significant sorption on MnO{sup 2}, TiO{sup 2} and activated carbon. On the other hand, in Mg O, Sn O and calcined hydrotalcite also was observed a sorption, although in smaller quantities. The studied hydrated metallic oxides retained the {sup 60} Co for ion exchange via. It was found that the {sup 60} Co was present as a cationic specie to p H 1, 3, 5 and 7 and like a neutral specie to alkaline p H. (Author)

  18. Irradiation treatment of sewage sludge: History and prospects

    International Nuclear Information System (INIS)

    This paper first reviews the history of irradiation treatment of sewage sludge in the world. The first sludge irradiation plant was built in Geiselbullach, West Germany in 1973 and used 60Co as irradiation source. Since then, many sludge irradiators were constructed in U.S.A., India, Japan, Canada, Poland, etc., which used 60Co, 137Cs or electron beam as irradiation sources. The paper then describes some basic research on irradiation treatment of sewage sludge including optimization of irradiation parameters, synergistic effect of radiation with heat, oxygenation, irradiation-composting and potential applications of treated sludge. Some proposals have been suggested for further development of this technology in the future

  19. History and prospects of irradiation treatment of sewage sludge

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    This paper presents a survey of irradiation treatment of sewage sludge in the world.Since the first sludge irradiation plant was built in Geiselbullach, West Germany in 1973 which used 60Co as irradiation source, many sludge irradiators were constructed in USA, India, Japan, Canada, Poland and so on, which used 60Co, 137Cs or electron beam as irradiation sources.Some basic researches on irradiation treatment of sewage sludge are, respectively, reviewed, including optimization of irradiation parameters, synergistic effect of radiation with heat, oxygenation, irradiation-composting and potential applications of treated sludge.Some proposals have been suggested for further development of this technology.

  20. Study of the action of 60Co gamma radiation on Salmonella poona, Escherichia coli and Alicyclobacillus acidoterrestris in mango pulp

    International Nuclear Information System (INIS)

    The application of non-thermal treatments has proven effective in inhibiting bacteria such as Salmonella and Escherichia coli. Mango is a fruit of national consumption with a great exportation potential. Meanwhile, outbreaks of food borne disease related to mango consumption caused mistrust on the degree of food security offered by the product. The objective of this work was to establish the radioresistance of bacteria Escherichia coli, Salmonella Poona and Alicyclobacillus acidoterrestris on the mango pulp by the calculation of the D10 values and to know the radiation effect on the sensory characteristics of the fruit pulp. The microbiological profile of frozen mango pulp available at the local market was also established using conventional methods of plating and Most Probable Number (MPN). The pulps experimentally inoculated with the bacteria listed above were irradiated with doses of 0, 1, 2, 3, 4 and 5kGy in a 60Co source. The sensory analysis was performed using a dose of 5 kGy, using the triangular test and the test of acceptance with hedonic scale. The results of this study show that the quality of mango pulp sell in the local market is not satisfactory in accordance with the standards established by the Brazilian law and the literature, showing the need of using other tools to achieve acceptable levels of quality. The D10 values obtained are in the range of 1.01 and 1.09kGy for E. coli ATCC 8739, 0.60 and 0.98kGy for S. poona and 0.72 e 0.88kGy for A. acidoterrestris respectively. The triangular test showed that a 5kGy radiation dose changed the sensory characteristics of mango pulp. Nevertheless, sensory analysis of a food product prepared with the irradiated pulp obtained good acceptance in the attributes of global appearance, flavor and aroma. (author)

  1. Study of the action of 60Co gamma radiation on Salmonella poona, Escherichia coli and Alicyclobacillus acidoterrestris in mango pulp

    International Nuclear Information System (INIS)

    The application of non-thermal treatments has proven effective in inhibiting bacteria such as Salmonella and Escherichia coli. Mango is a fruit of national consumption with a great exportation potential. Meanwhile, outbreaks of foodborne disease related to mango consumption caused mistrust on the degree of food security offered by the product. The objective of this work was to establish the radioresistance of bacteria Escherichia coli, Salmonella Poona and Alicyclobacillus acidoterrestris on the mango pulp by the calculation of the D10 values and to know the radiation effect on the sensory characteristics of the fruit pulp. The microbiological profile of frozen mango pulp available at the local market was also established using conventional methods of plating and Most Probable Number (MPN). The pulps experimentally inoculated with the bacteria listed above were irradiated with doses of 0, 1, 2, 3, 4 and 5 kGy in a 60Co source. The sensory analysis was performed using a dose of 5 kGy, using the triangular test and the test of acceptance with hedonic scale. The results of this study show that the quality of mango pulp sell in the local market is not satisfactory in accordance with the standards established by the Brazilian law and the literature, showing the need of using other tools to achieve acceptable levels of quality. The D10 values obtained are in the range of 1.01 and 1.09 kGy for E. coli ATCC 8739, 0.60 and 0.98 kGy for S. poona and 0.72 and 0.88 kGy for A. acidoterrestris respectively. The triangular test showed that a 5 kGy radiation dose changed the sensory characteristics of mango pulp. Nevertheless, sensory analysis of a food product prepared with the irradiated pulp obtained good acceptance in the attributes of global appearance, flavor and aroma. (author)

  2. Dynamic observation of polypide in semen and blood of rabbits infected with Toxoplasma tachyzoites

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Toxoplasma gondii (T. gondii) is one of the intracellular parasitized protozoa and may cause severe medical complications in fetus or immunocompromised individuals. T. gondii existed as tachyzoite during acute stage while as bradyzoite during chronic phase in human cells. To improve understanding of the prevention, diagnosis, and treatment of the disease, it is important to explore the distribution and fluctuation and other biological features of T. gondii in host. The trophozoite had been found in the saliva, blood or urine of the host.1-4 Some studies suggested the dynamic changes of circulating antibody and toxoplasma circulating antigen (TCA) either in blood or in urine.5,6 T. gondii in tissue or blood cannot be counted exactly under the microscope because it was only several micrometers in size and thus most of the studies were performed qualitatively by mouse inoculation or immunology methods. The quantitative fluorescent polymerase chain reaction (QF-PCR) and its application raised the possibility for dynamic observation of the polypide in the host.7,8 In this study, blood and semen were collected from the male rabbit model infected with toxoplasma tachyzoites and T. gondii was detected by QF-PCR quantitatively.

  3. Effect of the ionizing radiation of the {sup 60}Co on the radiological properties of guar gum and carboxymethylcellulose for the food industry use; Efeito da radiacao ionizante de {sup 60}Co sobre propriedades radiologicas da goma guar e carboximetilcelulose para uso na industria alimenticia

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Fernando Fabris; Mastro, Nelida Lucia del [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)

    2002-07-01

    Hydrosoluble polysaccharides or gums are long chain polymers that dissolve or disperse in water. When added to foods, they change rheological characteristics, stabilize emulsions, promote particle suspension, control crystallization and inhibit sineresis of processed foods. Guar gum is an hydro soluble polysaccharide obtained from the seed of Cyamopsis tetragonolobus. Carboxy-methylcellulose (CMC) is an homopolisaccharide prepared by the treatment of alkaline cellulose with sodium monochloroacetate. This work presents the results of the study of radiation effects on the viscosity of guar gum and CMC used for the food industry when irradiated with {sup 60} Co ionizing radiation. (author)

  4. Behavior of the sorption of 60 Co in aqueous solution on inorganic materials as function of p H

    International Nuclear Information System (INIS)

    The sorption of the 60 Co is evaluated in aqueous solution on Mg O, MnO2, TiO2, Sn O, activated carbon and hydrotalcite calcined as a function of the p H, using the method for lots and quantifying at the 60 Co for gamma spectrometry. Likewise it was explained the one behavior of the sorption of the 60 Co in the materials with base in the chemical species of this radioactive isotope in aqueous solution. The chemical species of the 60 Co in solution were identified by electrophoresis of high voltage for the different p H values. It was found that under the experimental conditions, the 60 Co showed a significant sorption on MnO2, TiO2 and activated carbon. On the other hand, in Mg O, Sn O and calcined hydrotalcite also was observed a sorption, although in smaller quantities. The studied hydrated metallic oxides retained the 60 Co for ion exchange via. It was found that the 60 Co was present as a cationic specie to p H 1, 3, 5 and 7 and like a neutral specie to alkaline p H. (Author)

  5. Detection and temporal variation of (60)Co in the digestive glands of the common octopus, Octopus vulgaris, in the East China Sea.

    Science.gov (United States)

    Morita, Takami; Otosaka, Shigeyoshi; Fujimoto, Ken; Nishiuchi, Kou; Kimoto, Katsunori; Yamada, Haruya; Kasai, Hiromi; Minakawa, Masayuki; Yoshida, Katsuhiko

    2010-08-01

    (60)Co were detected in common octopus specimens collected in the East China Sea in 1996-2005. The source of (60)Co has remained unclear yet. Stable isotope analyses showed that there was no difference in stable Co concentrations between octopus samples with (60)Co and without (60)Co. This result showed that the stable Co in the digestive gland of octopus potentially did not include a trace amount of (60)Co and the source of (60)Co existed independently. Furthermore, investigations of octopus in other area and other species indicated that the origin of the source of (60)Co occurred locally in the restricted area in the East China Sea and not in the coastal area of Japan. Concentrations of (60)Co have annually decreased with shorter half-life than the physical half-life. This decrease tendency suggests that the sources of (60)Co were identical and were temporary dumped into the East China Sea as a solid waste.

  6. Assessment of the mobility and bioavailability of 60 Co and 137 Cs in contaminated soils

    International Nuclear Information System (INIS)

    Results of a classical sequential chemical extraction procedure for 137 Cs in an acid Oxisol showed that after 3 years of contamination radiocesium remains potentially available for transfer processes: 40% bio-available, 20% mobile under oxidizing conditions and 40% bound to Fe and Mn oxides (available under reducing conditions). At this time, the transfer factor obtained in this soil was higher than values obtained in basic Oxisol and was higher than values obtained in soils from temperate climate areas. Seven years after the contamination, the 137 Cs distribution in this acid Oxisol have been changed as consequence of changes in soil properties: 8% bioavailable, 16% mobile under oxidizing conditions, 43% bound to Fe and Mn oxides and 33% strongly bound to soil compounds. Changes in the 137 Cs distribution in this soil were followed by reductions in soil to plant transfer factor. Between 1996 and 2000, the 137 Cs distribution, 137 Cs soil to plant transfer factor and soil properties in the basic Oxisol remained almost the same. The 60 Co distribution showed that Mn oxides is the main sink for this element and four years after contamination no 60 Co was detected as bioavailable or detectable in plants. In this study the use of an alternative sequential chemical extraction protocol to evaluate 60 Co and 137 Cs mobility under a large range of physico-chemical soil properties has shown to be very consistent with soil to plant transfer factors data for maize. The knowledge of bio-geochemical behavior of radionuclides in soil system can be used for the risk assessment in the case of nuclear accident or contamination scenarios. (author)

  7. Sensitivity of watermelon variety Bojura to mutant agents 60Co and EMS

    OpenAIRE

    Nikolay Velkov; Nasya Tomlekova; Fatma Sarsu

    2016-01-01

    A study on the sensitivity of watermelon variety Bojura to mutagenic agents was carried out in 2013-2014. The goal was to establish effective doses for mutagenic treatment of dry seeds with 60Co gamma rays (80, 100, 200, 250, 350 and 450 Gy) and swollen seeds with water for 24 hours were treated with ethyl methanesulfonate (EMS) at a concentration of 2%. Dominant mutations were not observed in the M1 generation. Morphological changes in 14 of 1395 M2 plants were observed. Phenotypic variation...

  8. Development and evaluation of a technique for in vivo monitoring of {sup 60}Co in the lungs

    Energy Technology Data Exchange (ETDEWEB)

    Mello, J.Q. de; Lucena, E.A.; Dantas, A.L.A.; Dantas, B.M., E-mail: bmdantas@ird.gov.br [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

    2015-07-01

    {sup 60}Co is a fission product of {sup 235}U and represents a risk of internal exposure of workers in nuclear power plants, especially those involved in the maintenance of potentially contaminated parts and equipment. The control of {sup 60}Co intake by inhalation can be performed through in vivo monitoring. This work describes the evaluation of a technique through the minimum detectable activity and the corresponding minimum detectable effective doses, based on biokinetic and dosimetric models of {sup 60}Co in the human body. The results allow to state that the technique is suitable either for monitoring of occupational exposures or evaluation of accidental intakes. (author)

  9. Impact of 60Co-γ-ray Sterilization of Tetracycline Hydrochloride Raw Materials%60Co-γ射线对盐酸四环素原料灭菌的研究

    Institute of Scientific and Technical Information of China (English)

    黄波; 赵全如; 刘莉萍

    2014-01-01

    Different doses of 60Co-γ rays were compared for radiation sterilization on raw materials of tetracycline hydrochloride. By checking stability data, the optimal radiation dose of 60Co-γ rays on tetracycline hydrochloride raw materials was 9 kGy, at which its physical and chemical detection indicators were in line with the requirements of USP, and the sterility test results met the requirements..%采用不同剂量的60Co-γ射线对盐酸四环素原料进行辐射灭菌,结合稳定性考察数据,最终确定盐酸四环素原料的60Co-γ射线的辐射剂量为9 kGy时,其理化检测指标符合USP的要求,且无菌检查符合要求。

  10. Comparison between a commercial and homemade ionization chamber for dosimetry of {sup 60}Co beams

    Energy Technology Data Exchange (ETDEWEB)

    Neves, Lucio P.; Perini, Ana P.; Caldas, Linda V.E., E-mail: lpneves@ipen.b, E-mail: aperini@ipen.b, E-mail: lcaldas@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    The Calibration Laboratory at IPEN/CNEN (LCI) has developed several ionization detectors for dosimetry in diagnostic radiology, radiation protection and radiotherapy. Recently, a cylindrical ionization chamber, with a sensitive volume of 1.06cm{sup 3}, was developed, and several tests were performed to characterize this ionization chamber for radiotherapy level. The results showed that its performance was within the recommended international limits. In order to complement the studies regarding the response of this ionization chamber, in this work, the chamber response was compared with that of a commercial ionization chamber Farmer PTW, model TN30011-1. The ionization chamber produced at LCI is made of PVC and PMMA. A special build-up cap for {sup 60}Co beams was made of acrylic, with 4.00 mm thickness. All tests of both ionization chambers were performed under the same conditions, allowing good geometrical reproducibility. The performed tests were: saturation, ion collection efficiency, polarity effect and chamber tilt. The results obtained in this comparison program were all within the international recommendations, and demonstrate a good agreement of the performance of the commercial and the homemade ionization chambers. From this comparison results and from previous data, it is possible to conclude that the ionization chamber produced at IPEN presents usefulness for dosimetric applications at radiotherapy level in {sup 60}Co beams.(author)

  11. Scattering factor evaluation and study of medical intervention following intake of 60Co

    International Nuclear Information System (INIS)

    Guidelines for the assessment of internal doses from monitoring suggest default measurement of uncertainties (i.e. lognormal scattering factor, SF) to be used for different types of monitoring data. In this paper, SF values have been evaluated for internal contamination due to 60Co in two cases using whole body counting data. SF values of 1.04 and 1.03 were obtained for case I and II, respectively while SF value of 1.03 was obtained using bioassay data for case I. SF evaluated is in good agreement with the default values given by IDEAS guidelines. The present study also presents the follow up study of a case I of 60Co internal contamination using whole body counting and bioassay analysis. The effect of medical intervention applied on the subject is studied. Medical intervention of d-Penicillamine (250 mg x 4 daily) was orally administered from 13th day of initial exposure for about a fortnight, which showed reduction of activity present by 33.4% through urine. (author)

  12. Ferrous sulphate (Fricke) dosimetry in a fast neutron and a 60Co radiotherapeutical beams

    International Nuclear Information System (INIS)

    Ferrous sulphate (Fricke) dosimetry was performed in the fast neutron beam produced at the U-120 cyclotron of the Institute of Nuclear Physics and in a 60Co gamma-ray therapeutical beam at the Centre of Oncology in Krakow. The G-value was measured for the neutron component in the mixed (neutron + gamma) field of the fast neutron beam, where the mean neutron energy is 5.6 MeV. This value is Gn = 8.1 ± 0.8. Track structure theory calculations were made using energy spectra of charged secondary particles generated in water by the MRC Hammersmith fast neutron beam (mean neutron energy 7.6 MeV, measured value of Gn = 9.4 ± 0.6), yielding the calculated value of Gn = 8.61. Fricke dosimetry of the 60Co beam indicates that the absolute value of gamma-ray dose at the Centre of Oncology may be underestimated by about 3% . 19 refs., 1 tab. (author)

  13. Sensitivity of watermelon variety Bojura to mutant agents 60Co and EMS

    Directory of Open Access Journals (Sweden)

    Nikolay Velkov

    2016-04-01

    Full Text Available A study on the sensitivity of watermelon variety Bojura to mutagenic agents was carried out in 2013-2014. The goal was to establish effective doses for mutagenic treatment of dry seeds with 60Co gamma rays (80, 100, 200, 250, 350 and 450 Gy and swollen seeds with water for 24 hours were treated with ethyl methanesulfonate (EMS at a concentration of 2%. Dominant mutations were not observed in the M1 generation. Morphological changes in 14 of 1395 M2 plants were observed. Phenotypic variations changes were the colour of the seed coat, chlorophyll disorders of cotyledons, leaves, petals, and alterations of the location of the fruit set in the central stem. Visible changes of the morphological characteristics of the fruit were not observed. The doses induced certain morphological changes, however, higher doses or combined gamma rays 60Co and EMS treatments would induce mutations more efficiently. Subsequent experiments are required to obtain mutants with changes that affect flowers and fruits. The results are important for increasing mutation efficiency in watermelon breeding.

  14. A 3D superposition pencil beam dose calculation algorithm for a 60Co therapy unit and its verification by MC simulation

    Science.gov (United States)

    Koncek, O.; Krivonoska, J.

    2014-11-01

    The MCNP Monte Carlo code was used to simulate the collimating system of the 60Co therapy unit to calculate the primary and scattered photon fluences as well as the electron contamination incident to the isocentric plane as the functions of the irradiation field size. Furthermore, a Monte Carlo simulation for the polyenergetic Pencil Beam Kernels (PBKs) generation was performed using the calculated photon and electron spectra. The PBK was analytically fitted to speed up the dose calculation using the convolution technique in the homogeneous media. The quality of the PBK fit was verified by comparing the calculated and simulated 60Co broad beam profiles and depth dose curves in a homogeneous water medium. The inhomogeneity correction coefficients were derived from the PBK simulation of an inhomogeneous slab phantom consisting of various materials. The inhomogeneity calculation model is based on the changes in the PBK radial displacement and on the change of the forward and backward electron scattering. The inhomogeneity correction is derived from the electron density values gained from a complete 3D CT array and considers different electron densities through which the pencil beam is propagated as well as the electron density values located between the interaction point and the point of dose deposition. Important aspects and details of the algorithm implementation are also described in this study.

  15. 125I粒子和60Co γ射线照射对A549及BEAS-2B细胞生物学效应的影响%The biological effects of 125I seeds and 60Co γ-rays on A549 and BEAS-2B cells

    Institute of Scientific and Technical Information of China (English)

    赵真真; 茅爱武; 王忠敏; 刘芬菊; 曹燕; 贾一平

    2015-01-01

    目的 探讨125I粒子和60Co γ射线对非小细胞肺癌(NSCLC) A549细胞和正常支气管上皮BEAS-2B细胞生物学效应的影响.方法 A549、BEAS-2B细胞均行125I粒子和60Coγ射线不同剂量照射;集落形成实验检测细胞存活分数;流式细胞术检测细胞周期和细胞凋亡率;Western blot检测凋亡相关蛋白的表达水平.结果 A549细胞在4、6、8 Gy照射时,125I粒子组细胞克隆存活分数较60Co组降低更明显(t=6.06、9.42、4.90,P<0.05).A549细胞在4 Gy时,G1期细胞比例125I粒子组为70.67%±1.49%,60Co组为59.59% ±0.71%(t=10.77,P<0.05);细胞凋亡率125I粒子组为18.09%±0.73%,60Co组为9.81%±0.16%(t=19.40,P<0.05).125I粒子照射明显上调Bax、cleaved Caspase-3蛋白的表达,同时下调Bcl-2蛋白的表达.但不同射线同一剂量或相同射线不同剂量下,BEAS-2B细胞的凋亡率及凋亡相关蛋白的表达无明显变化.结论 125I粒子持续低剂量率照射较60Co γ射线高剂量率照射抑制A549细胞增殖的效应更明显.Bcl-2/Bax蛋白比失衡,最终致Caspase-3蛋白的活化在125I粒子持续低剂量率照射抑制肿瘤细胞增殖的效应中可能发挥重要的作用.%Objective To investigate the biological effects of 125I seeds and 60Co γ-rays on the non-small cell lung cancer cells A549 and the normal bronchial epithelium cells BEAS-2B.Methods A549 and BEAS-2B cells were irradiated with 125I seeds and 60Co γ-rays.The survival fraction was detected by colony formation assay.The cell cycle and cell apoptotic ratio were detected by flow cytometry.The expression of cell apoptotic related proteins was examined by western blot.Results After irradiation with different doses,the survival of A549 cells irradiated with 125I seeds was lower than that irradiated with γ-rays (t =6.06,9.42,4.90,P <0.05).After irradiation with 4 Gy of 125I seeds and 60Co γ-rays,the G1 phase percentages of A549 cells were 70.67% ± 1.49% and 59.59% ± 0

  16. DNA double-strand break and apoptosis induction in human lymphocytes in different cycle cell phases by 60Co gamma rays and Bragg peak protons of a medical beam

    International Nuclear Information System (INIS)

    A comparative analysis is made of the regularities in the formation of DNA double-strand break and apoptosis induction in peripheral human blood lymphocytes in different cell cycle phases after 60Co gamma and extended Bragg peak proton irradiation. It is shown that the formation of apoptotic cells in a lymphocyte population increases linearly in all the cell cycle stages after proton irradiation. The maximal DNA double-strand break and apoptosis yield in lymphocytes is observed in the S phase of the cell cycle

  17. Bio-geochemical behavior of 90Sr, 137Cs and 60Co in tropical soil

    International Nuclear Information System (INIS)

    One manner to assess the bio-geochemical behavior of radionuclides in soils is the integration of experimental methods results obtained in the laboratory with results obtained in field experiments. In this work was used an alternative sequential chemical extraction protocol to evaluate mobility of radionuclides as a function of some physico-chemical conditions operationally defined: Slightly acidic phase: CH3COOH + CH3COONa 1:1, pH 4.7, shacked at room temperature; Easily reducible phase: NH2OH.HCl (0.1 M), pH 2, shacked at room temperature;Oxidizable phase: H2O2 (30%) + HNO3, pH 2, CH3COONH4 (1 M);shacked at room temperature; Alkaline phase: NaOH (0.1 M), pH 12, shacked at room temperature; Resistant phase: Aqua regia heated to 50 deg C / 30 min. The results obtained experimentally indicate the vulnerability of some Brazilian soils due to the higher radionuclide transfer to plant. Although it seems clear that it is difficult to identify which soil property will determine a given TF, the results of geochemical partition for 137Cs, 90Sr and 60Co obtained in slightly acidic phase were very consistent with the TF data for reference plants or with some of the soil properties recognized in the specialized literature as related with mechanisms of sorption of Cs (e.g. exchangeable K, organic matter and iron oxides content), Co (e.g. manganese oxide) and Sr (pH and exchangeable Ca). The 137Cs distribution in soil showed that Fe oxides are the main sink for this element in all type of soil and 16 years after contamination the 137Cs the TF remains almost the same in Goiania soil. The 60Co distribution in soil showed that Mn oxides are the main sink for this element in all type of soils and in the Nitisol, 5 years after contamination, the 60Co was not detected as bioavailable (in the slightly acidic phase) neither detected in plant or were detectable with values very close to the detection limit. In the Nitisol, it is possible that reduction in 137Cs transfer is also

  18. 60Co-γ辐射对家蚕生长发育的影响%Silkworm Growth under 60Co-γ Beam Radiation

    Institute of Scientific and Technical Information of China (English)

    孙辉; 刘朝良; 穆莉; 陈仕才; 李锋; 邵引刚; 汤良文; 周炎

    2008-01-01

    利用60Co-γ射线对家蚕卵进行照射,辐射剂量在10 Gy~30 Gy之间对蚕的生长发育等一系列变化进行研究.结果表明:随着辐射剂量的增加,家蚕在1小时内的平均孵化率有极显著提高,对照区的平均孵化率为33.2%,而10 Gy、20 Gy和30 Gy区分别达到34.44%、39.67%和51.11%.四龄第2天随机称量50头蚕,对照、10 Gy、20 Gy和30 Gy区每头蚕平均体重分别为1.174 g、1.116 g、0.975 g和0.862 g,体重随剂量增加明显减轻,其中20 Gy和30 Gy区对比对照和10 Gy区均有极显著差异;茧层率在10 Gy和20 Gy辐照区与对照区比较变化不大,即23.37%、23.54%对比24.25%,而30 Gy辐照区显著降低,为21.74%;单蛾产卵数先升高后降低,从对照区的529粒到10 Gy的540粒和30 Gy的392粒,这可能是辐照使蚕生长发育的机制发生了改变,引起膜损伤、破坏蛋白质的结构的原因,并且对蚕的消化功能也产生了影响.综合考虑在家蚕辐射育种方面10 Gy时的照射效果最佳.

  19. Measurements of (60)Co in massive steel samples exposed to the Hiroshima atomic bomb explosion.

    Science.gov (United States)

    Gasparro, Joël; Hult, Mikael; Marissens, Gerd; Hoshi, Masaharu; Tanaka, Kenichi; Endo, Satoru; Laubenstein, Matthias; Dombrowski, Harald; Arnold, Dirk

    2012-04-01

    To study discrepancies in retrospective Hiroshima dosimetry, the specific activity of (60)Co in 16 steel samples from Hiroshima was measured using gamma-ray spectrometry in underground laboratories. There is general agreement between these new activity measurements and the specific activities derived from previously calculated dose values on the one hand and former measurements of samples gathered at distances less than 1,000 m from the center of the explosion ( 1,300 m slant range) were mainly cosmogenically induced. Furthermore, at long range, these results are in disagreement with older measurements whose specific activity values were 10 to 100 times higher than predicted by computer model calculations in DS86 and DS02. As a consequence, the previously reported discrepancy is not confirmed. PMID:22378201

  20. EVALUATION OF THE MIGRATION POTENTIAL FOR 60Co AND 137Cs AT THE MAINE YANKEE SITE

    International Nuclear Information System (INIS)

    The objective of this report is to discuss the degree of sorption and desorption of 137Cs and 60Co that may be associated with the granite bedrock and the ''popcorn'' cement drain system that underlie the Maine Yankee Containment Foundation. The purpose is to estimate how much retardation of these two radionuclides takes place in groundwater that flows in the near-field of the Containment Foundation, specifically with respect to contamination originating at the PAB Test Pit. Specific concerns revolve around the potential for the contamination originating near the PAB to create a radioactive dose to a hypothetical ''resident farmer'' using a well intercepting this water to exceed 4 millirems/yr

  1. Quality of Intensity Modulated Radiation Therapy Treatment Plans Using a {sup 60}Co Magnetic Resonance Image Guidance Radiation Therapy System

    Energy Technology Data Exchange (ETDEWEB)

    Wooten, H. Omar, E-mail: hwooten@radonc.wustl.edu; Green, Olga; Yang, Min; DeWees, Todd; Kashani, Rojano; Olsen, Jeff; Michalski, Jeff; Yang, Deshan; Tanderup, Kari; Hu, Yanle; Li, H. Harold; Mutic, Sasa

    2015-07-15

    Purpose: This work describes a commercial treatment planning system, its technical features, and its capabilities for creating {sup 60}Co intensity modulated radiation therapy (IMRT) treatment plans for a magnetic resonance image guidance radiation therapy (MR-IGRT) system. Methods and Materials: The ViewRay treatment planning system (Oakwood Village, OH) was used to create {sup 60}Co IMRT treatment plans for 33 cancer patients with disease in the abdominal, pelvic, thorax, and head and neck regions using physician-specified patient-specific target coverage and organ at risk (OAR) objectives. Backup plans using a third-party linear accelerator (linac)-based planning system were also created. Plans were evaluated by attending physicians and approved for treatment. The {sup 60}Co and linac plans were compared by evaluating conformity numbers (CN) with 100% and 95% of prescription reference doses and heterogeneity indices (HI) for planning target volumes (PTVs) and maximum, mean, and dose-volume histogram (DVH) values for OARs. Results: All {sup 60}Co IMRT plans achieved PTV coverage and OAR sparing that were similar to linac plans. PTV conformity for {sup 60}Co was within <1% and 3% of linac plans for 100% and 95% prescription reference isodoses, respectively, and heterogeneity was on average 4% greater. Comparisons of OAR mean dose showed generally better sparing with linac plans in the low-dose range <20 Gy, but comparable sparing for organs with mean doses >20 Gy. The mean doses for all {sup 60}Co plan OARs were within clinical tolerances. Conclusions: A commercial {sup 60}Co MR-IGRT device can produce highly conformal IMRT treatment plans similar in quality to linac IMRT for a variety of disease sites. Additional work is in progress to evaluate the clinical benefit of other novel features of this MR-IGRT system.

  2. 五指毛桃水提液对60Coγ射线致小鼠骨髓细胞DNA损伤的防护作用%Protective Role of Aqueous Extract from Fici Hirtae Radix for DNA Damage of Bone Marrow Cells by 60Coγ-Ray in Mice

    Institute of Scientific and Technical Information of China (English)

    王晓平; 段丽菊; 黄翔; 岑业文; 李桂芬

    2011-01-01

    目的 探讨五指毛桃水提液对60Coγ射线所致的小鼠骨髓细胞DNA损伤的防护作用.方法 40只SPF级♂小鼠随机分为5组:阴性对照组给予生理盐水,辐射模型组单纯接受照射,3个五指毛桃剂量组分别给予相当于生药5,10,20 g.kg-1.d-1五指毛桃水提液,连续灌胃7d.除阴性对照组外,所有小鼠接受全身一次性60Coγ射线照射,照射剂量为6Gy.照射24 h后处死小鼠,取股骨,制作骨髓单细胞悬液,用单细胞凝胶电泳实验检测小鼠骨髓细胞DNA损伤情况.结果 辐射模型组小鼠骨髓细胞DNA的尾部DNA百分率(Tail DNA%)和尾矩(Tail Moment)高于阴性对照组(P<0.01),五指毛桃各剂量组的 Tail DNA%和Tail Moment均显著低于辐射模型组(P<0.01),且随着给药剂量的增加,Tail DNA%和Tail Moment降低.结论 五指毛桃水提液对60Coγ射线损伤小鼠骨髓细胞DNA具有一定的防护作用.%OBJECTIVE To investigate the protective role of aqueous extract from Fici Hirtae Radix for bone marrow cells DNA of 60Coγ-ray irradiated mice. METHODS Forty SPF male mice were randomized into 5 groups. Negative control group was given normal saline, irradiated group was radiation model group and 3 Fici Hirtae Radix dose groups were orally given aqueous extracts from Fici Hirtae Radix of 5, 10, 20 g·kg-1·d-1 for 7 consecutive days. Except the negative control group, all mice received 6 Gy irradiation of whole body. After 24 h of irradiation, mice were sacrificed on femur. Single cell suspension was produced. DNA damage was detected using single cell gel electrophoresis experiment. RESULTS The results showed that Tail DNA% and Tail Moment of radiation model mice were higher than negative control group (P<0.01). Tail DNA% and Tail Moment of all Fici Hirtae Radix dose groups were significantly lower than radiation model group(P<0.01). As the dose increased,Tail DNA% and Tail Moment reduced. CONCLUSION Aqueous extracts from Fici Hirtae Radix have

  3. Preliminary Study on the Quantitative Value Transfer Method of Absorbed Dose to Water in 60Co γ Radiation

    Directory of Open Access Journals (Sweden)

    SONG Ming-zhe

    2015-01-01

    Full Text Available Absorbed dose to water in 60Co γ radiation is the basic physics quantity in the quantitative value system of radiation therapy, it is very necessary for radiation therapy. The study on the quantitative value transfer method of absorbed dose to water in 60Co γ Radiation could provide important technical support to the establishment of Chinese absorbed dose to water quantity system. Based on PTW-30013 ionization chamber, PMMA water phantom and 3D mobile platform, quantitative value transfer standard instrument was established, combined with the requirement of IAEA-TRS398, developed preliminary study of 60Co absorbed dose to water quantity value transfer method. After the quantity value transfer, the expanded uncertainty of absorbed dose to water calibration factor of PTW-30013 was 0.90% (k=2, the expanded uncertainty of absorbed dose to water of 60Co γ reference radiation in Radiation Metrology Center (SSDL of IAEA was 1.4% (k=2. The results showed that, this value transfer method can reduce the uncertainty of 60Co absorbed dose to water effectively in Secondary Standard Dosimetry Laboratory.

  4. The accumulation and distribution of 60Co in carp (Cyprinus carpio) in water-fish compartment system

    International Nuclear Information System (INIS)

    In relation with nuclear safety assessment, the parameter of radionuclide transfer in the environment is significantly needed for internal doses estimation received by public trough environment - food product - human pathways. International Atomic Energy Agency (IAEA) has published the transfer parameter data for temperate zone in Technical Report Series 472. In order to complete the IAEA data, especially for tropical region, the accumulation and distribution of 60Co in carp (Cyprinus carpio) was experimentally studied based on the water-fish compartment system. The carp were cultured in 500L water containing 60Co of about 30 Bq.mL-1 in a water tank. The 60Co concentration in fish and water were measured using gamma spectrometer. The 60Co was accumulated and distributed in the fish tissues with the concentration ratio (CR) of 3.08 mL.g-1 1.55 mL.g-1 and 1.14 mL.g-1 for internal organs, bones, and muscle, respectively. The CR of 60Co in the fish will be useful in internal radiation dose estimation to human trough water-fish-human pathway, and will also complete the IAEA transfer parameter data for tropical region. (author)

  5. Quality of Intensity Modulated Radiation Therapy Treatment Plans Using a (60)Co Magnetic Resonance Image Guidance Radiation Therapy System

    DEFF Research Database (Denmark)

    Wooten, H Omar; Green, Olga; Yang, Min;

    2015-01-01

    PURPOSE: This work describes a commercial treatment planning system, its technical features, and its capabilities for creating (60)Co intensity modulated radiation therapy (IMRT) treatment plans for a magnetic resonance image guidance radiation therapy (MR-IGRT) system. METHODS AND MATERIALS......: The ViewRay treatment planning system (Oakwood Village, OH) was used to create (60)Co IMRT treatment plans for 33 cancer patients with disease in the abdominal, pelvic, thorax, and head and neck regions using physician-specified patient-specific target coverage and organ at risk (OAR) objectives. Backup...... plans using a third-party linear accelerator (linac)-based planning system were also created. Plans were evaluated by attending physicians and approved for treatment. The (60)Co and linac plans were compared by evaluating conformity numbers (CN) with 100% and 95% of prescription reference doses...

  6. Soil to plant transfer of {sup 137}Cs and {sup 60}Co in Ferralsol, Nitisol and Acrisol

    Energy Technology Data Exchange (ETDEWEB)

    Wasserman, M.A. [Instituto de Radioprotecao e Dosimetria, CNEN, Av. Salvador Allende s/no, Recreio, CEP: 22780-160, Rio de Janeiro, RJ (Brazil)], E-mail: angelica@ird.gov.br; Bartoly, F.; Viana, A.G.; Silva, M.M.; Rochedo, E.R.R. [Instituto de Radioprotecao e Dosimetria, CNEN, Av. Salvador Allende s/no, Recreio, CEP: 22780-160, Rio de Janeiro, RJ (Brazil); Perez, D.V. [Centro Nacional de Pesquisa de Solos, EMBRAPA, R. Jardim Botanico 1024, CEP: 22460-000, Rio de Janeiro, RJ (Brazil); Conti, C.C. [Instituto de Radioprotecao e Dosimetria, CNEN, Av. Salvador Allende s/no, Recreio, CEP: 22780-160, Rio de Janeiro, RJ (Brazil)

    2008-03-15

    In this study, soil to plant transfer factor values were determined for {sup 137}Cs and {sup 60}Co in radish (Raphanus sativus), maize (Zea mays L.) and cabbage (Brassica oleracea L. var. capitata) growing in gibbsite-, kaolinite- and iron-oxide-rich soils. After 3 years of experiment in lysimeters it was possible to identify the main soil properties able to modify the soil to plant transfer processes, e.g. exchangeable K and pH, for {sup 137}Cs, and organic matter for {sup 60}Co. Results of sequential chemical extraction were coherent with root uptake and allowed the recognition of the role of iron oxides on {sup 137}Cs behaviour and of Mn oxides on {sup 60}Co behaviour. This information should provide support for adequate choices of countermeasures to be applied on tropical soils in case of accident or for remediation purposes.

  7. Biochemical detection of E-ADA on Neospora caninum tachyzoites and the effects of a specific enzymatic inhibitor

    Directory of Open Access Journals (Sweden)

    Alexandre A. Tonin

    2015-01-01

    Full Text Available Objective. This study aimed to investigate the presence and activity of the ecto adenosine deaminase (E-ADA enzyme in tachyzoites of Neospora caninum (Nc-1 strain, as well as to assess the activity of a well-known E-ADA inhibitor, the deoxycoformycin. Materials and methods. The parasites were grown in cell culture, being subsequently separated in a pellet of tachyzoites, on which the E-ADA activity was tested using the concentrations 0 (control, 0.2, 0.4 and 0.8 mg mL-1. Results. The E-ADA showed high activity, progressively increasing its activity according to the enhancement of the protein concentration. The test was carried out with different concentrations of deoxycoformycin, showing that it was able to inhibit the E-ADA present on the free form of the parasite. Conclusions. Based on these results we conclude that the E-ADA is present on tachyzoites of N. caninum, and deoxycoformycin is able to inhibit this enzyme. In this sense, knowing the negative impact of N. caninum on reproductive issue in cattle (mainly abortion, might it is an alternative in order to deal with this parasitic infection.

  8. The Behaviour of 134Cs, 60Co, and 85Sr Radionuclides in Marine Environmental Sediment

    Directory of Open Access Journals (Sweden)

    Nariman H.M. Kamel

    2002-01-01

    Full Text Available This work describes experimental investigations and modelling studies on the sorption of radionuclides 134Cs, 60Co, and 85Sr by certain marine sediments within Egypt. The chemical composition of the marine sediments was determined. The soluble salts were measured for the sediments and the concentrations of the released cations, Al3+, Fe3+, and Si4+, were measured for the sediment materials in 0.1 M NaClO4 aqueous solution at different hydrogen ion concentrations. The two main factors that control the uptake of the radionuclides onto the sediment are the pH and the exchangeable capacities of the sediment materials. Surface complex model was used to estimate the surface charge densities and the electric surface potential of the marine sediment materials. These two parameters were calculated at the surface capacity sites of the sediment materials. The desorption of the adsorbed cations was determined by means of selective consecutive extraction tests using different chemical reagents including (1 1 M MgCl2 (pH 7, (2 1 M ammonium oxalate (pH 3-5, (3 0.04 M NH2OH,HCl in 25% acetic acid (pH 3-4, (4 H2O2 in 5% HNO3(pH 2-3, and (5 digestion with nitric acid followed by hydrofluoric and perchloric acids (pH 2.

  9. 129I, 60Co, and 106Ru measurements on water samples from the Hanford project environs

    International Nuclear Information System (INIS)

    Groundwater flow and contamination patterns beneath the Hanford project reservation have been studied since the early days of the project. The measurement of radioactive materials at concentrations much below those required for radiation protection are useful for tracing groundwater movement and detection of potential contamination problems before they are apt to occur. Groundwater samples from a number of wells on or near the Hanford reservation have been analyzed for 129I by neutron activation analysis and for gamma radioactivity by low-level coincidence gamma-ray spectrometry. The major radionuclides in addition to natural radioactivity detected in the underground waters by gamma-ray spectrometry were 106Ru and 60Co. Local river and rain water samples were also analyzed for 129I and long-lived radionuclides. Special sample collection methods were developed to prevent contamination of the water samples during collection. Anions travel farther than cations in underground water systems since soils are primarily cation exchangers and retain the cations. Anion exchange techniques were used in the field and the laboratory to recover the desired radionuclides. Sample sizes ranged up to several thousand liters. This paper discusses the sample collection methods,analysis methods, and results obtained. The methods used were found to provide high sensitivity for groundwater studies. (auth)

  10. 100 Gy 60Co γ-Ray Induced Novel Mutations in Tetraploid Wheat

    Directory of Open Access Journals (Sweden)

    Chuntao Yang

    2014-01-01

    Full Text Available 10 accessions of tetraploid wheat were radiated with 100 Gy 60Co γ-ray. The germination energy, germination rate, special characters (secondary tillering, stalk with wax powder, and dwarf, meiotic process, and high-molecular-weight glutenin subunits (HMW-GSs were observed. Different species has different radiation sensibility. With 1 seed germinated (5%, T. dicoccum (PI434999 is the most sensitive to this dose of radiation. With a seed germination rate of 35% and 40%, this dose also affected T. polonicum (As304 and T. carthlicum (As293. Two mutant dwarf plants, T. turgidum (As2255 253-10 and T. polonicum (As302 224-14, were detected. Abnormal chromosome pairings were observed in pollen mother cells of both T. dicoccoides (As835 237-9 and T. dicoccoides (As838 239-8 with HMW-GS 1Ax silent in seeds from them. Compared with the unirradiated seed of T. polonicum (As304 CK, a novel HMW-GS was detected in seed of T. polonicum (As304 230-7 and its electrophoretic mobility was between 1By8 and 1Dy12 which were the HMW-GSs of Chinese Spring. These mutant materials would be resources for wheat breeding.

  11. A review of the (60)Co internal dosimetry at Devonport Royal Dockyard.

    Science.gov (United States)

    Vickers, J M A; Collison, R; Collision, R

    2010-03-01

    The physico-chemical properties of (60)Co contaminants arising from the UK Naval Nuclear Propulsion Programme (NNPP) pressurised water reactor (PWR) plants have been investigated in order to review individual monitoring requirements at Devonport Royal Dockyard (DRD). This has been achieved through laboratory tests on NNPP primary component samples and interpretation of direct bioassay measurements using internal dosimetry modelling software. Interpretation of lung measurements was completed for two inhalation events involving material originating from a PWR plant and post-primary circuit decontamination. Initial estimates of intake and dose were calculated using International Commission on Radiological Protection default parameter values. However, a good fit could only be achieved by fitting the data to alternative absorption parameters where 90-95% of the material dissolved and absorbed rapidly at a rate of 1 day(-1). As a consequence of this review, a number of improvements have been made to monitoring arrangements at DRD. A minimum of three direct measurements are now taken during the 0-30 day period after an intake, the capability of the Canberra Accuscan has been enhanced and dissolution tests are being carried out by the Health Protection Agency (HPA) on samples taken from PWR plants. PMID:20220215

  12. A review of the {sup 60}Co internal dosimetry at Devonport Royal Dockyard

    Energy Technology Data Exchange (ETDEWEB)

    Vickers, J M A; Collision, R [Babcock Marine, Devonport Royal Dockyard, Plymouth PL1 4SG (United Kingdom)], E-mail: Jenny.Vickers@babcock.co.uk, E-mail: Roger.Collison@babcock.co.uk

    2010-03-01

    The physico-chemical properties of {sup 60}Co contaminants arising from the UK Naval Nuclear Propulsion Programme (NNPP) pressurised water reactor (PWR) plants have been investigated in order to review individual monitoring requirements at Devonport Royal Dockyard (DRD). This has been achieved through laboratory tests on NNPP primary component samples and interpretation of direct bioassay measurements using internal dosimetry modelling software. Interpretation of lung measurements was completed for two inhalation events involving material originating from a PWR plant and post-primary circuit decontamination. Initial estimates of intake and dose were calculated using International Commission on Radiological Protection default parameter values. However, a good fit could only be achieved by fitting the data to alternative absorption parameters where 90-95% of the material dissolved and absorbed rapidly at a rate of 1 day{sup -1}. As a consequence of this review, a number of improvements have been made to monitoring arrangements at DRD. A minimum of three direct measurements are now taken during the 0-30 day period after an intake, the capability of the Canberra Accuscan has been enhanced and dissolution tests are being carried out by the Health Protection Agency (HPA) on samples taken from PWR plants.

  13. Cloning and Clone Analysis of GRA1 Gene from Local Isolate Toxoplasma gondii Tachyzoite

    Directory of Open Access Journals (Sweden)

    Didik T Subekti

    2008-03-01

    Full Text Available The GRA1 gene of Toxoplasma gondii encoding protein called GRA1 protein. GRA1 protein known to be immunogenic and essentialy involved in modification of parasitophorus vacoule which has role in immune evasion and virulency of organism. The local isolate of T. gondii is successfuly isolated and known as highly pathogenic isolate similarly as its RH strain. Unfortunately, the homology sequence of GRA1 gene between those isolate still unknown. The purpose of the research are to clone the GRA1 gene and to analyze the homology from pathogenic T. gondii isolate and RH strain. Tachyzoite of T. gondii was grown in mice peritoneum by intraperitoneal injection. Then, total mRNA was isolated and purified. cDNA was synthesized from mRNA and then amplified using F1 dan R1 primers to get clone of GRA1 from local isolate. Homology analysis was perform using several bioinformatic softwares. The result showed that cDNA of GRA1 from local isolate has 84% homologs with RH strain of T.gondii. However, when subsequently editing performed to parts of suspected non coding sequence of cDNA GRA1 to get CDS of GRA1, the homology was increase to 100% compare to CDS of GRA1 of RH strain.

  14. Macrophages facilitate the excystation and differentiation of Toxoplasma gondii sporozoites into tachyzoites following oocyst internalisation.

    Science.gov (United States)

    Freppel, Wesley; Puech, Pierre-Henri; Ferguson, David J P; Azas, Nadine; Dubey, Jitender P; Dumètre, Aurélien

    2016-01-01

    Toxoplasma gondii is a common parasite of humans and animals, which is transmitted via oocysts in cat faeces or tissue cysts in contaminated meat. The robust oocyst and sporocyst walls protect the infective sporozoites from deleterious external attacks including disinfectants. Upon oocyst acquisition, these walls lose their integrity to let the sporozoites excyst and invade host cells following a process that remains poorly understood. Given the resistance of the oocyst wall to digestive enzymes and the ability of oocysts to cause parenteral infections, the present study investigated the possible contribution of macrophages in supporting sporozoite excystation following oocyst internalisation. By using single cell micromanipulations, real-time and time-point imaging techniques, we demonstrated that RAW macrophages could interact rapidly with oocysts and engulfed them by remodelling of their actin cytoskeleton. Internalised oocysts were associated to macrophage acidic compartments and showed evidences of wall disruption. Sporozoites were observed in macrophages containing oocyst remnants or in new macrophages, giving rise to dividing tachyzoites. All together, these results highlight an unexpected role of phagocytic cells in processing T. gondii oocysts, in line with non-classical routes of infection, and open new perspectives to identify chemical factors that lead to oocyst wall disruption under physiological conditions. PMID:27641141

  15. Macrophages facilitate the excystation and differentiation of Toxoplasma gondii sporozoites into tachyzoites following oocyst internalisation

    Science.gov (United States)

    Freppel, Wesley; Puech, Pierre-Henri; Ferguson, David J. P.; Azas, Nadine; Dubey, Jitender P.; Dumètre, Aurélien

    2016-01-01

    Toxoplasma gondii is a common parasite of humans and animals, which is transmitted via oocysts in cat faeces or tissue cysts in contaminated meat. The robust oocyst and sporocyst walls protect the infective sporozoites from deleterious external attacks including disinfectants. Upon oocyst acquisition, these walls lose their integrity to let the sporozoites excyst and invade host cells following a process that remains poorly understood. Given the resistance of the oocyst wall to digestive enzymes and the ability of oocysts to cause parenteral infections, the present study investigated the possible contribution of macrophages in supporting sporozoite excystation following oocyst internalisation. By using single cell micromanipulations, real-time and time-point imaging techniques, we demonstrated that RAW macrophages could interact rapidly with oocysts and engulfed them by remodelling of their actin cytoskeleton. Internalised oocysts were associated to macrophage acidic compartments and showed evidences of wall disruption. Sporozoites were observed in macrophages containing oocyst remnants or in new macrophages, giving rise to dividing tachyzoites. All together, these results highlight an unexpected role of phagocytic cells in processing T. gondii oocysts, in line with non-classical routes of infection, and open new perspectives to identify chemical factors that lead to oocyst wall disruption under physiological conditions. PMID:27641141

  16. Study on the energy dependence of gamma radiation detectors for {sup 137}Cs and {sup 60}Co; Estudo da dependencia energetica de detectores de radiacao gama para {sup 137}Cs e {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Nonato, Fernanda B.C.; Diniz, Raphael E.; Carvalho, Valdir S.; Vivolo, Vitor; Caldas, Linda V.E., E-mail: fbnonato@ipen.b, E-mail: rediniz@ipen.b, E-mail: vcsouza@ipen.b, E-mail: vivolo@ipen.b, E-mail: lcaldas@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2009-07-01

    38 Geiger-Mueller radiation detectors and 9 ionization chambers were calibrated, viewing to study the energy dependence of the monitor response for gamma radiation fields ({sup 137}Cs and {sup 60}Co). The results were considered satisfactory only for ionization chambers and for some Geiger-Mueller detectors

  17. Evaluation of the ionizing radiation effects of the {sup 60}Co on the physical, chemical and nutritional properties of Phaseolus vulgaris L. e Vigna unguiculata (L.) Walp beans; Avaliacao dos efeitos da radiacao ionizante de {sup 60}Co em propriedades fisicas, quimicas e nutricionais dos feijoes Phaseolus vulgaris L. e Vigna unguiculata (L.) Walp

    Energy Technology Data Exchange (ETDEWEB)

    Villavicencio, Anna Lucia Casanas Haasis

    1998-07-01

    The effects of {sup 60} Co ionizing radiations in doses of 0; 0.5; 1.5; 2.5; 5.0 and 10 kGy on beans, Phaseolus vulgaris L., of the carioca variety and Vigna unguiculata (L.) Walp, of the macacar variety stored for 6 months were studied. The cooking time was established, and then the following analyses were carried out: Sensory, vitamins B{sub 1}, B{sub 2} and B{sub 6} protein content, biological evaluation in rats (Food intake and Weight gain (in grams)), apparent Digestibility (Dapp), apparent Net Protein Utilization (NPUapp) and apparent Biological Value (BVapp), as well as the applicability of detection methods of irradiated foodstuffs through germination tests, the analysis of DNA migration, thermoluminescence and analysis of the carbohydrates formed by radiation. Changes in the cooking time were observed for all doses. In doses up to 1 kGy, the nutritional quality of the irradiated beans were not altered. The application of the proposed detection methods of the irradiated foodstuffs allowed the detection of irradiated beans with doses as low as 0.5 kGy. (author)

  18. Comparison of the standards of absorbed dose to water of the OMH and the BIPM for 60Co γ rays

    International Nuclear Information System (INIS)

    A comparison of the standards of absorbed dose to water of the Orszagos Meresugyi Hivatal (OMH), Budapest, Hungary and of the Bureau International des Poids et Mesures (BIPM) has been made in 60Co radiation. The results show that the OMH and the BIPM standards for absorbed dose to water are in close agreement, the difference being within the estimated uncertainty. (authors)

  19. Immobilization of 60Co and 90Sr ions using red mud from aluminum industry

    Directory of Open Access Journals (Sweden)

    Milenković Aleksandra S.

    2014-01-01

    Full Text Available The removal of 60Co and 90Sr from the aqueous phase was tested using red mud - the fine grained residue from bauxite ore processing. This industrial waste represents a mixture of numerous minerals, mainly oxides and hydroxides of Fe, Al, Si, and Ti. Experiments were conducted as a function of contact time, pH, and pollutant concentrations. Kinetic data were well fitted with a pseudo-second order equation. The calculated rate constants and initial sorption rates indicated faster sorption of Sr2+ ions. Removal of both cations rapidly increased with the initial pH increase from 2.5 to 3.5. With the further increase of pH, Co2+ sorption was nearly constant (98%-100%, whereas Sr2+ removal remained at the same level to initial pH ~8 and gradually increased to 100% at pH 12. Equilibrium sorption data followed the Langmuir model, with the maximum sorption capacities of 0.52 mmol/g for Co2+ and 0.31 mmol/g for Sr2+. Sorbed cations exhibited high stability in distilled water. Desorption of Co2+ was also negligible in the presence of the competing Ca2+ cation, while 42%-25% of Sr2+ ions were desorbed depending on the previously sorbed amount. The results indicate that red mud is of potential significance as Co2+ and Sr2+ immobilization agent due to its high efficiency, abundance, and low-cost. [Projekat Ministarstva nauke Republike Srbije, br. 43009

  20. Diffusion of strongly sorbing cations (60Co and 152Eu) in compacted Febex bentonite

    International Nuclear Information System (INIS)

    Document available in extended abstract form only. Compacted bentonite is used as an engineered barrier in high-level radioactive waste (HLRW) repositories because is a swelling clay of very low permeability and high sorption capability for many solutes. The transport of radionuclides through compacted bentonite is a diffusion-controlled process retarded by sorption. Performance assessment calculations of a repository need diffusion coefficients data of relevant radionuclides. Several studies on diffusion behaviour of neutral, anionic and weakly sorbing elements on clay exist while very few studies are available for moderately sorbing elements, and almost no studies for Eu, a highly sorbing element are reported. In this study, diffusion experiments with strongly sorbing radionuclides, as 60Co and 152Eu, have been performed through compacted FEBEX bentonite. Diffusion essays with these strongly sorbing radionuclides are not straightforward to carry out because they are very time consuming essays, but also because sorption on the diffusion cells, tubing, filters and reservoirs, typically used in the classical through-diffusion or in-diffusion methods make hard the interpretation of the experimental results and the calculation of the diffusion coefficients. FEBEX bentonite was selected as Spanish reference buffer materials, and used in many national and international projects. The clay comes from the Cortijo de Archidona deposit (Almeria, Spain), and has a smectite content greater than 90% (93 ± 2%), with quartz (2 ± 1%), plagioclase (3 ± 1%), cristobalite (2 ± 1%), potassic feldspar, calcite, and trydimite as accessory minerals. The specific weight of the FEBEX bentonite is 2.7 g/cm3. Diffusion experiments were performed using the instantaneous plane source method. In this setup, a paper filter tagged with a tracer is introduced between two compacted tablets, avoiding contact between the tracer and the experimental vessels. The tracer can diffuse into both

  1. Preparation of Low Activity 60Co Sealed Source Based on Zirconyl Molybdo-Pyro phospate Inorganic Ion Exchanger for Radiometric Calibration Purposes

    International Nuclear Information System (INIS)

    Zirconyl Molybdo-pyrophosphate inorganic ion exchanger was prepared and used for preparation of a 60Co sealed source. The corresponding distribution coefficients of 60Co(II) ions on Zirconyl Molybdo-pyrophosphate were determined as a function of HCl acid concentration. The apparent capacity of Zirconyl Molybdo-pyrophosphate for 60Co(II) was determined by dynamic method and it was found to be 0.24 mmol/g. Zirconyl Molybdo-pyrophosphate was loaded with 60Co radionuclides by equilibrating of 60Co solution with 1g of it at ph 5 (HCl) for 48 h at 25±1 degree C. Sealed source of 1.0 8% μ Ci 60Co was prepared by packing 100 mg of the loaded matrix in the cylindrical cavity of a Chinese artelone capsule. The sealed source was submitted to quality control tests.

  2. Effects of 60Co radiation on the rabbit lung surfactant system

    International Nuclear Information System (INIS)

    Rabbits were irradiated under sodium pentobarbital anethesia with 3,000 rad of 60Cobalt radiation in either a single dose or five fractions. At different intervals over one month post-irradiation, an ultrastructural study of the alveolar membrane and alveolar macrophages was performed; lung lavage fluid was studied for phosopholipid weight and composition, and for surface tension properties. With both radiation schedules, a surface tension defect of the pulmonary washings, a transitory weight increase of the alveolar phospholipids and the development of an interstitial edema could be observed. The surface tension defect could be explained by ultrastructural changes in surfactant and changes in alveolar phospholipid weight or composition at early times after irradiation (2 and 7 days). However, this explanation is invalid for late effects (28 days). The proposed hypothesis is an impairment of other components of surfactant system

  3. A 60Co multipurpose radiation processing facility at Bahia Blanca, Argentina

    Science.gov (United States)

    Curzio, O. A.; Croci, C. A.

    The aim of the project is to have a multipurpose facility which will enable us to show the techno-economic viability of the irradiation process applied to regional products, important from the economic point of view. The topics will fundamentally be connected with regional themes such as food preservation and the modification of polymer structures. This project will make it possible to carry out basic and applied studies related to radiation chemistry, dosimetry and engineering irradiation processes. The facility will operate in the Universidad Nacional del Sur (UNS) with a maximum activity of 18.5 PBq of Co-60. The viability and design of the irradiation facility is supported by the Government of the Buenos Aires Province since it is interested in the socio-economic benefit of this technology at the regional level.

  4. The regional genomic instability induced by 60Co γ-rays in B16 cells transfected by GFP%60Coγ射线诱导GFP转染的B16细胞区域性基因组不稳定性改变的研究

    Institute of Scientific and Technical Information of China (English)

    刘静; 王亚婷; 林海; 韩倩; 张春晓; 白欧

    2012-01-01

    Objective To detect the regional genomic instability of B16 cells treated with 60Co γ-rays by a green fluorescence protein (GFP)-based genomic instability reporting system.Methods Three groups were employed as non-transfection group,vector control group and transfection group.The GFP-marked reporter construct pCMV-EGFP2XhoI for regional genomic instability was successfully transfected into B16 cells using liposome.B16 cells were selected by screening of G418 with a series of concentrations and limiting dilution cultures to yield a single colony.B16 cells with the genomic instability report system were then irradiated by 60Co γ-rays at doses of 0,2 and 4 Gy.The regional genomic instability of B16 cellswas quantified by counting the number of cells with GFP expression.Results B-16 cell strain steadilyexpressing the GFP-based genomic instability reporting system was established successfully.GFP-positiveB16 cells were observed at 1 d after irradiation with 60Co γ-rays at doses of 2 and 4 Gy.Positive correlations between fluorescence intensity and dose and fluorescence intensity and time were also observed.The positive expression rate of GFP followed the increased of dose (F =36.55,36.76,P < 0.05) and time (t =-3.27,-3.16,-4.26,-6.11,-7.17,P < 0.05),and differences between groups were significant.The positive expression rate of GFP increased significantly at 3 d,and maximum expression was observed at 5 d(2.46 ± 0.24 and 3.82 ± 0.35).The level was tending towards stability.Spontaneous GFP expression at a ratio of 1/600 000 was observed in 0 Gy group after 2 weeks of culture.Conclusions The regional genomic instability of B16 cells induced by 60Co γ-rays can be detected using a GFP-labelled genomic instability reporter system.%目的 应用绿色荧光蛋白(GFP)标记的基因组不稳定性报告系统,检测60Co γ射线诱导B16细胞区域性基因组不稳定性改变.方法 实验分为3组:未转染组、转染组及转染对照组.应用脂质体转染

  5. Use of 60 Co gamma radiation to expend the shell life of packaged sliced loaves

    International Nuclear Information System (INIS)

    The evaluation of conservation of sliced loaves (bread cut into slices). baked by five bakeries of Piracicaba, after gamma irradiation and maintained into polyethylene begs of low density, of 47,5 and 85 μm of thicknesses is shown. The sliced loaves were put into the bags and thermo-sealed by hand, like they were handled by the bakers. After this, they were irradiated with doses of 0.0: 2.0; 4.0; 6.0; 8.0 and 10.0 kGy of gamma radiation in a irradiation chamber of Cobalt-60 at a dose rate of 2,68 kGy per hour, at the room temperature (280 C). After irradiation the samples were maintained under at the room temperature (26 - 340 C), and humidity, as similar as possible to the conditions of the markets, bakeries and shops they were sold. The samples were evaluated every days and if any of them presented sign of contamination. It was threw away because it was inappropriate for human consumption. (author)

  6. On-line {sup 60}Co monitor for reactor recirculation system piping in primary containment vessel during reactor operation

    Energy Technology Data Exchange (ETDEWEB)

    Ueno, Katsunori, E-mail: katsunori.ueno.pa@hitachi.com [Hitachi Research Laboratory, Hitachi, Ltd., Hitachi 319-1224, Ibaraki (Japan); Tadokoro, Takahiro [Hitachi Research Laboratory, Hitachi, Ltd., Hitachi 319-1224, Ibaraki (Japan); Tsuyuki, Mizuho; Matsubara, Hirofumi; Ota, Nobuyuki; Nagase, Makoto [Hitachi-GE Nuclear Energy, Ltd., Hitachi 317-0073, Ibaraki (Japan)

    2014-10-15

    Highlights: • We developed an on-line {sup 60}Co monitor for reactor recirculation system piping during reactor operation. • Energy resolution at 1.4 × 10{sup 6} cps is 33 keV at 1332 keV using a pulse integral method. • A coincidence method is applied to reduce an effect of background gamma rays. • The coincidence counting of {sup 60}Co cascade gamma rays could be detected for a background dose rate of 4.8 mSv/h. - Abstract: Water chemistry control during reactor operation and installation of temporary radiation shielding prior to scheduled outages are carried out in order to reduce workers’ dose exposure caused by {sup 60}Co which is the main radiation source during scheduled outages of boiling water reactor (BWR) power plants. It is necessary to monitor the deposited {sup 60}Co on inner surfaces of reactor recirculation system (RRS) piping to evaluate effects of water chemistry control. We have developed an on-line {sup 60}Co monitor (OLCM) for this purpose. The OLCM applies a pulse integral method as a new method to measure gamma-ray counts of more than 1.0 × 10{sup 6} counts per second (cps) and a coincidence counting method to reduce an effect of background gamma rays caused by {sup 16}N in the measurement of {sup 60}Co cascade gamma rays. Energy resolution at 1.4 × 10{sup 6} cps is 33 keV using the pulse integral method and single LaBr{sub 3}:Ce scintillation detector. The energy resolutions of this detector using the fast response photo multiplier tube (PMT) are 50 keV at 1.5 × 10{sup 6} cps and 59 keV at 2.1 × 10{sup 6} cps. Furthermore, we measured the energy spectra using the pulse integral method, the coincidence counting method and two LaBr{sub 3}:Ce scintillation detectors and examined the transition of coincidence counting for {sup 60}Co detection under high dose rate during reactor operation at the Kyoto University Research Reactor (KUR). The coincidence counting of {sup 60}Co cascade gamma rays could be detected, and the result was

  7. Public knowledge and attitudes towards the 60Co incident in Qixian County, Henan Province

    International Nuclear Information System (INIS)

    Objective: To investigate radiation risk perception and nuclear acceptance after the 60Co stuck source incident in June 2009 in Qixian County, Henan Province. Methods: From March to April 2010, 23 hamlets and 2 schools from 5 towns in Qixian County were selected, while 3 hamlets and 2 schools from 3 towns were used as control in Huixian County, 135 km away where no any impact of the incident was found. Face-to-face questionnaire survey was conducted among three groups including mass public, elite public (representatives of public opinion, such as physicians, teachers, governmental officials, administrative personnel, private entrepreneurs), and middle school students (the third grade students in junior middle schools and the first grade students in senior middle schools). Results: A total of 1 340 valid questionnaires were collected. The public knowledge levels about radiation and nuclear power were low among all groups in two areas and public knowledge levels in Qixian County were higher than those in Huixian County. In both areas.the knowledge levels of the students were higher than those among the elite public, and that among the general public were the lowest. The knowledge levels of males and those with higher educational levels were higher than those of females and those with lower educational levels. More than 40.0% of the respondents supported the idea to build nuclear power plants in China, but only a small part of them supported to build nuclear power plants in their own areas. The percentages of opponents against local nuclear power plant construction were especially higher among the students and those with higher educational levels. More than 60% of the mass public and elite public believed the explanation of the government. More than 80% of the respondents held negative attitudes towards the performance of the Qixian County Government in dealing with this incident. The student gave the lowest evaluation of the credibility and the specific performance

  8. The 1997 determination of the Australian standards of exposure and absorbed dose at {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Huntley, R.B.; Boas, J.F. [Australian Radiation Laboratory, Yallambie, VIC (Australia); Van der Gaast, H. [Australian Nuclear Science and Technology Organisation, Lucas Heights, NSW (Australia)

    1998-05-01

    The arrangements for the maintenance of the Australian standards for {sup 60}Co are described in detail. The primary standards are a graphite cavity chamber for exposure/air kerma and a graphite calorimeter for absorbed dose. These secondary standards are described and their responses in corresponding {sup 90}Sr reference sources are reported. Accurate ratios between the Australian Radiation Laboratory (ARL) and Australian Nuclear Science and Technology (ANSTO) {sup 90}Sr reference sources are derived for use in future calibrations. The value of 28.8 years for the half-life of {sup 90}Sr is confirmed. The usefulness of {sup 90}Sr reference source measurements in quality assurance is discussed. The charge sensitivity and linearity of the ANSTO electrometers are reported by two different methods and are compared with previous results. Calibration factors for all the secondary standard ionization chambers are given, in terms of exposure, air kerma and absorbed dose to water. Calibration factors are also given for most of the chambers in terms of absorbed dose to graphite. The methods of deriving the calibration factors are explained in detail, including all the corrections applied to both the primary and secondary standard measurements. Three alternative methods of deriving the absorbed dose to water calibration factors are compared. The reported calibration factors are compared with previous results. Changes in the Australian units of exposure, air kerma and absorbed dose to graphite and water are derived from changes in the corresponding calibration factors. The Australian units of exposure and air kerma have not changed significantly since 1990. The Australian unit of absorbed dose to graphite is now 1.1 % smaller than in 1993 and 1.3 % smaller than in 1990. The Australian unit of absorbed dose to water is now 1.4 % smaller than in 1993, but is only 0.9 % smaller than in 1990. Comparisons of the Australian standards of exposure/air kerma and absorbed dose with

  9. Radiosensibilidad de semillas de soya a los rayos gamma 60Co

    Directory of Open Access Journals (Sweden)

    C. de la Fé

    2000-01-01

    Full Text Available El presente estudio se desarrolló en las instalaciones del Instituto Nacional de Ciencias Agrícolas, con el objetivo de evaluar la radiosensibilidad de semillas de soya a los rayos gamma 60Co. Para su ejecución, se seleccionaron e irradiaron muestras de 50 semillas de la variedad Cubasoy-23, las que fueron sembradas en condiciones semicontroladas (macetas en la campaña de primavera, 1997. Las dosis de radiación fueron desde los 50 hasta los 480 Gy, con incrementos de 50 Gy entre las dosis de 50 y 200 Gy e incrementos de 40 Gy entre esta última y la dosis de 480 Gy. Las evaluaciones realizadas consistieron en porcentaje de semillas germinadas a los tres, seis y nueve días de la siembra, altura de las plantas a los 10, 20 y 50 días y número de pares de hojas emitidas a los 10 y 20 días de la siembra. El estudio realizado evidenció la ocurrencia de un efecto fuerte de las dosis aplicadas en el porcentaje de semillas germinadas, resultando las dosis próximas a los 280 Gy las de mayor efecto radioestimulador de la germinación. Desde el punto de vista de la altura de las plantas, el efecto de las dosis aplicadas se correspondió con el descrito para otros materiales biológicos irradiados, caracterizándose este por un aumento a dosis bajas seguido por una reducción cada vez mayor a medida que las dosis aplicadas se incrementaron. Por su parte, la emisión de hojas por las plantas mantuvo un comportamiento en correspondencia con la altura de las plantas. Los resultados del estudio permiten sugerir la aplicación de dosis próximas a los 360 Gy en los trabajos dirigidos a la creación de variabilidad genética en programas de mejora por inducción de mutaciones

  10. Sorption of {sup 60}Co and {sup 137}Cs in sediments at Piraquara de Fora, Angra dos Reis, RJ, Brazil; Sorcao de {sup 60}Co e {sup 137}Cs em sedimentos do Saco de Piraquara de Fora - Angra dos Reis

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Franciane M.; Martins, Nadia S.F.; Lauria, Dejanira D., E-mail: francian@ird.gov.br, E-mail: nadia@ird.gov.br, E-mail: dejanira@ird.gov.br [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil); Ferreira, Ingryd M., E-mail: ingrydmarques@hotmail.com [Instituto Federal do Rio de Janeiro (IFRJ), RJ (Brazil); Azevedo, Izabella M.A.P., E-mail: izabella_azevedo@id.uff.br [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil)

    2013-07-01

    In this study, sediment of Piraquara de Fora, located near the launch area of the effluents of the Central Nuclear Almirante Alvaro Alberto - CNAAA Nuclear Power Plants, were collected. It were determined the parameters particle size and cationic exchange capacity (CTC). From the sorption curves of {sup 60}Co and {sup 137}Cs, obtained experimentally in the laboratory, were estimated preliminary values of Kd at equilibrium, for these radionuclides in order of magnitude of 10{sup 2}.

  11. Radiation Effects of 60Co γ-Rays on Expression of CDKN1A mRNA in Human Lymphocytoblast%60Co γ射线照射正常人淋巴母细胞诱导CDKN1A基因mRNA表达水平的变化

    Institute of Scientific and Technical Information of China (English)

    朱昊; 施展; 何颖; 沈先荣; 王国卿

    2011-01-01

    Objective To study effect of 60Co γ-rays radiation with different doses on expression of CDKN1A gene mRNA in human lymphocytoblasl cultured for different time. Methods After human lym-phocytoblasts were irradiated by the 60Co γ-rays with various doses of 0, 0. 2, 1,3,5 and 10 Gy,and cells were separately cultured for sustaining survival during 0, 4, 24, 48, 72 and 96 h. The total RNA was extracted from each sample and the real-time PCR was conducted to observe the gene CDKN1A mR-NA level changes in lymphocytoblasts exposed to various radiation doses for various cultured time. Results Expressive levels of the CDKNIA mRNA in lymphocytoblasts gradually went up with increasing radiation doses, which showed γ-rays dose dependent from 0 Gy to 5 Gy (P <0. 05), and reach the peak when cells were cultured for 24 h after exposing to radiation while displayed an expressive downtrend during the later stage of cell culture. Conclusion Increase of the CDKN1A mRNA expression level in human lymphocytoblasts after exposing to 60Co γ-rays radiation within 24 hours of culture shows a dose dependent way, which may be used to evaluate the ionizing radiation dose.%目的 探讨不同剂量60Co γ射线在不同培养时间后对人淋巴母细胞AHH-1细胞CDKNIA基因mRNA表达的影响.方法 用不同剂量(0、0.2、1、3、5、10Gy)的60Coγ射线照射正常人淋巴母细胞AHH-1细胞,在不同维持存活培养时间(0、4、24、48、72、96 h)内收集细胞,抽提总RNA,用实时PCR方法检测细胞中CDKNIA基因mRNA表达水平,观察其随辐照剂量和培养时间的变化.结果 AHH-1细胞中CDKN1A基因 mRNA的表达水平随辐照剂量的加大而增加,在0~5Gy之间具有一定剂量依赖性关系(P<0.05);辐照后培养24 h时基因表达达到峰值,24h之后呈现下降趋势.结论 不同剂量γ射线照射人AHH-1细胞会导致CDKNIA基因表达水平在培养24h内随辐射剂量增加而升高,这种剂量依赖性关系可能适用于电离辐射剂量的估算.

  12. Effects of 60Co gamma radiations on the conduction properties of frog sciatic nerve

    International Nuclear Information System (INIS)

    The effect of Co60 gamma radiations on the amplitude, detection period and duration of the action potential of sciatic nerves of Rana tigrina frogs have been studied. Dose levels of upto 120 kR were employed. Irradiation of the nerves with dose levels upto 30 kR does not seem to affect the action of potential profiles. The amplitude falls rapidly thereafter, registering less than 5% of the original value after irradiation by a dose of 120 kR. Qualitatively similar behaviour is seen in the case of detection period and duration of action potential. These results are examined in the light of data already collected through experiments on the effect of Co60 gamma rays on the enzyme acetyl-cholinesterase (AchE). (author)

  13. Effects of 60Co gamma-rays, ultraviolet light, and mitomycin C on Halobacterium salinarium and Thiobacillus intermedius.

    Science.gov (United States)

    Shahmohammadi, H R; Asgarani, E; Terato, H; Ide, H; Yamamoto, O

    1997-03-01

    Lethal effects of 60Co gamma-rays, UV light, and mitomycin C on two kinds of bacteria, Halobacterium salinarium which grows in highly concentrated salt media and Thiobacillus intermedius which requires reduced sulfur compounds, were studied and compared with those on Escherichia coli B/r. D37 values for H. salinarium, T. intermedius and E. coli B/r were 393, 150, and 92 Gy, respectively, by exposure to 60Co gamma-rays. They were 212, 38, and 10 J/m2, respectively, by exposure to UV light and 2.36, 0.25, and 0.53 microgram/ml/h, respectively, by exposure to mitomycin C. Against these agents, H. salinarium was much more resistant than T. intermedius and E. coli B/r.

  14. Fast timing study of a CeBr3 crystal: Time resolution below 120 ps at 60Co energies

    International Nuclear Information System (INIS)

    We report on the time response of a novel inorganic scintillator, CeBr3. The measurements were performed using a cylindrical crystal of 1-in. in height and 1-in. in diameter at 22Na and 60Co photon energies. The time response was measured against a fast reference BaF2 detector. Hamamatsu R9779 and Photonis XP20D0 fast photomultipliers (PMTs) were used. The PMT bias voltages and Constant Fraction Discriminator settings were optimized with respect to the timing resolution. The Full Width at Half Maximum (FWHM) time resolution for an individual CeBr3 crystal coupled to Hamamatsu PMT is found here to be as low as 119 ps at 60Co energies, which is comparable to the resolution of 107 ps reported for LaBr3(Ce). For 511 keV photons the measured FWHM time resolution for CeBr3 coupled to the Hamamatsu PMT is 164 ps.

  15. Effects of {sup 60}Co gamma-rays, ultraviolet light, and mitomycin C on halobacterium salinarium and thiobacillus intermedius

    Energy Technology Data Exchange (ETDEWEB)

    Shahmohammadi, H.R.; Asgarani, E.; Terato, Hiroaki; Ide, Hiroshi; Yamamoto, Osamu [Hiroshima Univ. (Japan)

    1997-03-01

    Lethal effects of {sup 60}Co {gamma}-rays, UV light, and mitomycin C on two kinds of bacteria, Halobacterium salinarium which grows in highly concentrated salt media and Thiobacillus intermedius which requires reduced sulfur compounds, were studied and compared with those on Escherichia coli B/r. D{sub 37} values for H. salinarium, T. intermedius and E. coli B/r were 393, 150, and 92 Gy, respectively, by exposure to {sup 60}Co {gamma}-rays. They were 212, 38, and 10 J/m{sup 2}, respectively, by exposure to UV light and 2.36, 0.25, and 0.53 {mu}g/ml/h, respectively, by exposure to mitomycin C. Against these agents, H salinarium was much more resistant than T. intermedius and E. coli B/r. (author)

  16. Sorption of 60Co on a synthetic titanosilicate analogue of the mineral penkvilksite-2O and antimonysilicate

    International Nuclear Information System (INIS)

    The ability of two different types of synthetic inorganic ion-exchangers to sorb radioactive cobalt-60 using a batch-type method was studied. The two materials examined were the analogue of the natural titanosilicate penkvilksite-2O (AM-3) and a synthetic antimonysilicate. Ion-exchange experiments were performed with solutions labelled with radioactive cobalt (60Co). The sorption of 60Co onto the two samples materials were compared in terms of distribution coefficient (Kd), sorption percentage and cobalt quantity removed in mg per gram weight of the material. Several parameters were investigated viz. contact time, cobalt concentration, and sorbent concentration. It was found that the batch factor and cobalt concentration had a significant influence on the sorption of cobalt onto both of the materials. This was associated with the difference in pH generated by suspensions of the materials in water which was alkaline for the penkvilksite-2O analogue, and acid for the synthetic antimonysilicate. (author)

  17. Investigation of the /sup 59/Co(n,. gamma. )/sup 60/Co reaction with unpolarized and polarized neutrons

    Energy Technology Data Exchange (ETDEWEB)

    Kopecky, J. (Stichting Energieonderzoek Centrum Nederland, Petten. Nuclear Structure Group); Delfini, M.G. (Stichting Energieonderzoek Centrum Nederland, Petten. Nuclear Structure Group; Rijksuniversiteit Utrecht (Netherlands). Fysisch Lab.); Chrien, R.E. (Brookhaven National Lab., Upton, NY (USA))

    1984-10-15

    Capture of 24 keV neutrons and polarized as well as unpolarized thermal neutrons in /sup 59/Co has been investigated. Of the 350 ..gamma..-rays observed 335 could be placed in a /sup 60/Co level scheme containing 144 levels. The reaction Q-value amounts to 7491.92(8) keV. The chi/sup 2/ analysis of the circularly polarized ..gamma..-rays results in 31 unambiguous spin assignments for /sup 60/Co levels. The fraction of the J=4/sup -/ channel in the thermal capture was determined to be (80 +- 1)%. An average ..gamma..-ray multiplicity of 2.3 gammas per neutron capture was observed. The resulting level scheme is compared to shell-model predictions. Possible reaction mechanisms are discussed.

  18. Correction factors for Farmer-type chambers for absorbed dose determination in 60Co and 192Ir brachytherapy dosimetry

    International Nuclear Information System (INIS)

    This paper presents experimentally determined correction factors for Farmer-type chambers for absorbed dose determination in 60Co and 192Ir brachytherapy dosimetry. The correction factors were determined from measurements made in a PMMA phantom and calculation of ratios of measured charges. The ratios were corrected for the different volumes of the ionization chambers, determined in external high-energy electron beams. The correction factors for the central electrode effect and the wall material dependency in 60Co brachytherapy dosimetry agree with those used in external 60Co beam dosimetry. In 192Ir dosimetry, the central aluminium electrode increases the response of an NE2571 chamber compared with that of a chamber with a central graphite electrode. The increase is 1.1 and 2.1% at 1.5 and 5.0 g cm-2 distance, respectively. Similar values are obtained with an NE2577 chamber. The wall correction factor in 192Ir dosimetry for a chamber with an A-150 wall has been determined to be 1.018, independent of the measurement distance. For a graphite walled chamber, the correction factor is 0.996 and 1.001 at 1.5 and 5.0 g cm-2 distance, respectively. The values of the wall correction factors are evaluated by a theory presented. If the chamber is used according to the 'large cavity' principle, the correction factor to account for the replacement of the phantom material by the ionization chamber was determined to be 0.982 for an NE2571 chamber when used with a Delrin cap, and 0.978 for an NE2581 when used with a polystyrene cap. The correction factors for the 'large cavity' principle are valid at both 60Co and 192Ir qualities. (author)

  19. The Lowest Spin and Parity Levels on Two Particle System for Odd-oddNuclei 60Co and 46K

    International Nuclear Information System (INIS)

    For obtaining the lowest spin and parity levels of odd-odd nuclei, theanalyzing of the nuclei 60Co and 46K has been done using delta forcemodel. The calculation is done by theoretically and compared with experiment.To get a result optimally, the data analyzed using least square method. It isshown that the lowest spin and parity level from calculation result and theexperiment result are similar. (author)

  20. Combined reactor neutron beam and 60Co γ-ray radiation effects on CMOS APS image sensors

    Directory of Open Access Journals (Sweden)

    Zujun Wang

    2015-02-01

    Full Text Available The combined reactor neutron beam and 60Co γ-ray radiation effects on complementary metal-oxide semiconductor (CMOS active pixel sensors (APS have been discussed and some new experimental phenomena are presented. The samples are manufactured in the standard 0.35-μm CMOS technology. Two samples were first exposed to 60Co γ-rays up to the total ionizing dose (TID level of 200 krad(Si at the dose rates of 50.0 and 0.2 rad(Si/s, and then exposed to neutron fluence up to 1 × 1011 n/cm2 (1-MeV equivalent neutron fluence. One sample was first exposed to neutron fluence up to 1 × 1011 n/cm2 (1-MeV equivalent neutron fluence, and then exposed to 60Co γ-rays up to the TID level of 200 krad(Si at the dose rate of 0.2 rad(Si/s. The mean dark signal (KD, the dark signal non-uniformity (DSNU, and the noise (VN versus the total dose and neutron fluence has been investigated. The degradation mechanisms of CMOS APS image sensors have been analyzed, especially for the interaction induced by neutron displacement damage and TID damage.

  1. Combined reactor neutron beam and 60Co γ-ray radiation effects on CMOS APS image sensors

    International Nuclear Information System (INIS)

    The combined reactor neutron beam and 60Co γ-ray radiation effects on complementary metal-oxide semiconductor (CMOS) active pixel sensors (APS) have been discussed and some new experimental phenomena are presented. The samples are manufactured in the standard 0.35-μm CMOS technology. Two samples were first exposed to 60Co γ-rays up to the total ionizing dose (TID) level of 200 krad(Si) at the dose rates of 50.0 and 0.2 rad(Si)/s, and then exposed to neutron fluence up to 1 × 1011 n/cm2 (1-MeV equivalent neutron fluence). One sample was first exposed to neutron fluence up to 1 × 1011 n/cm2 (1-MeV equivalent neutron fluence), and then exposed to 60Co γ-rays up to the TID level of 200 krad(Si) at the dose rate of 0.2 rad(Si)/s. The mean dark signal (KD), the dark signal non-uniformity (DSNU), and the noise (VN) versus the total dose and neutron fluence has been investigated. The degradation mechanisms of CMOS APS image sensors have been analyzed, especially for the interaction induced by neutron displacement damage and TID damage

  2. Leaching of 60Co and 137Cs from spent ion exchange resins in cement–bentonite clay matrix

    Indian Academy of Sciences (India)

    I B Plecas; R S Pavlovic; S D Pavlovic

    2003-12-01

    The leaching rate of 60Co and 137Cs from the spent cation exchange resins in cement–bentonite matrix has been studied. The solidification matrix was a standard Portland cement mixed with 290–350 (kg/m3) spent cation exchange resins, with or without 2–5% of bentonite clay. The leaching rates from the cement–bentonite matrix for 60Co: (4,2–7,0) × 10-5 (cm/d) and 137Cs: (3,2–6,6) × 10-4 (cm/d), after 125 days were measured. From the leaching data the apparent diffusivity of cobalt and cesium in cement–bentonite clay matrix with a waste load of 290–350 (kg/m3) spent cation exchange resins, was measured for 60Co: (1,1–4,0) × 10-6 (cm2/d) and 137Cs: (0,5–2,6) × 10-4 (cm2/d), after 125 days. The results presented in this paper are part of the results obtained in a 20-year mortar and concrete testing project which will influence the design of radioactive waste management for a future Serbian radioactive waste disposal centre.

  3. A comparison of the biological effects of 125I seeds continuous low-dose-rate radiation and 60Co high-dose-rate gamma radiation on non-small cell lung cancer cells.

    Directory of Open Access Journals (Sweden)

    Zhongmin Wang

    Full Text Available To compare the biological effects of 125I seeds continuous low-dose-rate (CLDR radiation and 60Co γ-ray high-dose-rate (HDR radiation on non-small cell lung cancer (NSCLC cells.A549, H1299 and BEAS-2B cells were exposed to 125I seeds CLDR radiation or 60Co γ-ray HDR radiation. The survival fraction was determined using a colony-forming assay. The cell cycle progression and apoptosis were detected by flow cytometry (FCM. The expression of the apoptosis-related proteins caspase-3, cleaved-caspase-3, PARP, cleaved-PARP, BAX and Bcl-2 were detected by western blot assay.After irradiation with 125I seeds CLDR radiation, there was a lower survival fraction, more pronounced cell cycle arrest (G1 arrest and G2/M arrest in A549 and H1299 cells, respectively and a higher apoptotic ratio for A549 and H1299 cells than after 60Co γ-ray HDR radiation. Moreover, western blot assays revealed that 125I seeds CLDR radiation remarkably up-regulated the expression of Bax, cleaved-caspase-3 and cleaved-PARP proteins and down-regulated the expression of Bcl-2 proteins in A549 and H1299 cells compared with 60Co γ-ray HDR radiation. However, there was little change in the apoptotic ratio and expression of apoptosis-related proteins in normal BEAS-2B cells receiving the same treatment.125I seeds CLDR radiation led to remarkable growth inhibition of A549 and H1299 cells compared with 60Co HDR γ-ray radiation; A549 cells were the most sensitive to radiation, followed by H1299 cells. In contrast, normal BEAS-2B cells were relatively radio-resistant. The imbalance of the Bcl-2/Bax ratio and the activation of caspase-3 and PARP proteins might play a key role in the anti-proliferative effects induced by 125I seeds CLDR radiation, although other possibilities have not been excluded and will be investigated in future studies.

  4. Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate

    Directory of Open Access Journals (Sweden)

    Murwantoko M

    2015-11-01

    Full Text Available Rhoptry protein belongs to an excretory and secretory antigens (ESAs that play an important role during active penetration of parasite into the cell target. This protein an able Toxoplasma gondii to actively penetrate targeted cell, meanwhile ESAs protein stimulates intracellular vacuole modification. It is, therefore, after the parasite successfully enter the cell target then Granule (GRA proteins are responsible for the formation of parasitophorus vacuole, which is protect the fusion with other intracellular compartments such as lysosomal vacuole. Consequently, this parasite is being able to survive and multiply at the cell target. The current study was aimed to clone and sequens cDNA encoding for ROP-2 of local isolated T. gondii tachizoite through DNA recombinant technique. Total ribonucleic acid (RNA was isolated from tachyzoites of local isolated T. gondii that were grown up in Balb/c mice. Messenger RNA was isolated from total RNA using PolyAtract mRNA Isolation System. Messenger RNA was used as a template for synthesis cDNA using Riboclone cDNA Synthesis System AMV-RT. EcoRI adaptor from Riboclone EcoRI Adaptor Ligation System was added to Complementary DNA and than ligated to pUC19. Recombinant plasmid was transformed into E. coli (XL1-Blue. The transformed E. coli XL-1 Blue were plated on LB agar containing X-Gal, IPTG and ampicillin. Recombinant clones (white colony were picked up and grown up in the LB medium at 37oC overnight. Expression of recombinant protein was analysed by immunoblotting in order to identify cDNA recombinant wich is express ESA of T. gondii local isolate. Recombinant plasmid were isolated using alkalilysis method and were elektroforated in 1% agarose gel. The isolated DNA recombinant plasmid was cut using Eco RI and then sequenced through Big Dye Terminator Mix AB1 377A Sequencer using M13 Forward and M13 Reverse primers. The conclusion of this results showed that the recombinant clone was coding for excretory

  5. Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate

    Directory of Open Access Journals (Sweden)

    Wayan T. Artama

    2015-10-01

    Full Text Available Rhoptry protein belongs to an excretory and secretory antigens (ESAs that play an important role during activepenetration of parasite into the cell target. This protein an able Toxoplasma gondii to actively penetrate targetedcell, meanwhile ESAs protein stimulates intracellular vacuole modification. It is, therefore, after the parasitesuccessfully enter the cell target then Granule (GRA proteins are responsible for the formation of parasitophorusvacuole, which is protect the fusion with other intracellular compartments such as lysosomal vacuole. Consequently,this parasite is being able to survive and multiply at the cell target. The current study was aimed to clone andsequens cDNA encoding for ROP-2 of local isolated T. gondii tachizoite through DNA recombinant technique.Total ribonucleic acid (RNA was isolated from tachyzoites of local isolated T. gondii that were grown up in Balb/c mice. Messenger RNA was isolated from total RNA using PolyAtract mRNA Isolation System. Messenger RNA wasused as a template for synthesis cDNA using Riboclone cDNA Synthesis System AMV-RT. EcoRI adaptor fromRiboclone EcoRI Adaptor Ligation System was added to Complementary DNA and than ligated to pUC19. Recombinantplasmid was transformed into E. coli (XL1-Blue. The transformed E. coli XL-1 Blue were plated on LB agarcontaining X-Gal, IPTG and ampicillin. Recombinant clones (white colony were picked up and grown up in theLB medium at 37oC overnight. Expression of recombinant protein was analysed by immunoblotting in order toidentify cDNA recombinant wich is express ESA of T. gondii local isolate. Recombinant plasmid were isolatedusing alkalilysis method and were elektroforated in 1% agarose gel. The isolated DNA recombinant plasmid wascut using Eco RI and then sequenced through Big Dye Terminator Mix AB1 377A Sequencer using M13 Forward andM13 Reverse primers. The conclusion of this results showed that the recombinant clone was coding for excretoryand secretory

  6. Comparative effects of protracted exposures to 60Co γ-radiation and 239Pu α-radiation on breeding performance in female mice

    International Nuclear Information System (INIS)

    Breeding performances are compared of hybrid female mice given 239Pu(5 or 10μCikg-1 body mass in 1 per cent trisodium citrate via the tail-vein), or kept in a 10rad/day or 20rad/day 60Co γ-irradiation field (but mated in the control area), or unirradiated. Ovarian dose-rates from the injected plutonium were initially 0.8 and 1.7 rad/day, changing little thereafter; actual γ-ray dose-rates to breeding females averaged around 8 and 16 rad/day respectively. Both γ-ray treatments affected reproductive performance more than the plutonium injections, with respect to duration of fertility and to offspring per litter in successive 4-weekly periods, though overall mean litter-sizes were not significantly less than controls. The r.b.e. for these effects on reproduction, attributed to germ-cell killing, is about 2.5 for the α particles vs. γ-rays, lower than for testis mass reduction in males. This low r.b.e. may be connected with inhomogeneity of α-particle dose within the ovary, but it is known that fission neutron versus gamma r.b.e.'s for impairment of female fertility are also lower than those for impairment of male fertility. (Author)

  7. Effect of gamma radiation (60Co) on the growth of Aspergillus ochraceus coffee (Coffea arabica L.)

    International Nuclear Information System (INIS)

    Coffee is a major product on the world market. Its acceptance depends strongly on the sensory characteristics of the beverage, that is its aroma and flavor. One of the most important aspects of coffee culture today is the cup quality as such all segments of coffee production network are concentrating efforts to improve this trait. Foods that have been contaminated with fungi may contain may produce many mycotoxins including ochratoxim A (OTA). To prevent OTA in foodstuffs, it is necessary detect the producing fungi early. Ionizing radiation is a safe, environmentally clean, energy efficient process that can be used to increase the quality and reducing the microbiological contamination of the coffee. The aim of the present study was to evaluate the effects of different gamma radiation doses (0.0, 6.0, 12.0 and 18.0 kGy) on the growth of Aspergillus ochraceus in coffee (Coffee arabica L.). The analysis were performed to determine the fungi contamination the results were expressed as the viable counts per gram of sample (CFU/g) coffee samples irradiated and unirradiated. The results shows that microbiological contamination of coffee disagrees when increase doses of irradiation. The radiation doses 6.0, 12.0 and 18.0 kGy used resulted in a elimination of the number of Aspergillus ochraceus CFU/g when compared to the nonirradiated control group. Under the present conditions, gamma radiation was found to be an alternative for the control of Aspergillus ochraceus. (author)

  8. Dose rate effect in food irradiation

    International Nuclear Information System (INIS)

    It has been suggested that the minor losses of nutrients associated with radiation processing may be further reduced by irradiating foods at the high dose rates generally associated with electron beams from accelerators, rather than at the low dose rates typical of gamma irradiation (e.g. 60Co). This review briefly examines available comparative data on gamma and electron irradiation of foods to evaluate these suggestions. (137 refs., 27 tabs., 11 figs.)

  9. Study about the sensibility in vitro of different strains of Vibrio cholera 01 exposed to 60 Co gamma radiation; Estudo da sensibilidade in vitro de diferentes cepas de Vibio cholerae 01 a radiacao gama de 60Co

    Energy Technology Data Exchange (ETDEWEB)

    Moraes, Ivany Rodrigues de [Instituto Adolfo Lutz, Sao Paulo, SP (Brazil). Servicos de Saude; Gelli, Dilma Scala; Jakabi, Miyoko [Instituto Adolfo Lutz, Sao Paulo, SP (Brazil). Secao de Microbiologia; Mastro, Nelida Lucia del [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)]. E-mail: nlmastro@net.ipen.br

    1998-07-01

    The presence of some microorganisms in food, or the metabolites originated during their own multiplication may bring several diseases to humans: intoxications and food borne infections. Among the agents that may cause those diseases, we find Vibrio cholerae 01. In this experiment, the studies are focused on the radiosensibility in vitro of four strains of V. cholerae 01, exposed to different doses of ionizing radiation of {sup 60} Co. The results are compared with other data related to bacterial food borne diseases, including water. (author)

  10. A prototype of an ionization chamber for gamma radiation beams of 60Co: Experimental and Monte Carlo preliminary results

    International Nuclear Information System (INIS)

    Ionization chambers are the most widely used instruments for dosimetry in radiotherapy. With the aim to test new configurations and materials using low-cost and easily-available components, verify the possibility of its application in the gamma radiation field of 60Co and fulfill the need of a chamber for scientific metrological purposes, in this paper the prototype of a plane-parallel ionization chamber has been designed and built, and its performance has been studied at the SSDL of KARAJ. The front wall and back wall of the chamber were made of graphite and Plexiglas respectively, as opposed to the one type of material in commercially available chambers. The collecting electrode has a diameter of 20 mm. The sensitive volume is 0.63 cm3. It was found that the Leakage current, the short-term stability and the polarity effect were within the international recommendations. The results were compared with those of a reference cylindrical chamber. The maximum difference observed in this comparison was 1.1%. The relative uncertainty was below 0.2%. Moreover, Monte Carlo simulation was undertaken using MCNP4C code and the relative difference of 1.9% was observed compared to the experiment. As a result the chamber presented a satisfactory performance in all evaluated tests in Gamma radiation field of 60Co. -- Highlights: • The prototype of a plane-parallel Ionization Chamber was designed. • Its performance was studied in Gamma radiation field of 60Co. • The response of the chamber was measured and compared with that of the cylindrical ionization chamber. • The chamber was simulated using the MCNP4C Monte Carlo code. • The Leakage current, the short-term stability and the polarity effect were within the international recommendations

  11. A new cane variety-Guitang No.22 bred by 60Co γ-ray induced mutation

    International Nuclear Information System (INIS)

    Guitang No.22 was developed through seed stems of Xintaitang No.1 exposed at a dosage of 80 Gy 60Co γ-radiation. In Guangxi regional variety test, the results show that the cane yield and the sugar yield are about 106.95 and 14.21 t/hm2 respectively. Moreover, the highest tonnage of biomass and fermentable carbohydrates reaches about 188.1 and 48.92 t/hm2, respectively. Guitang 22 is a new variety which has the characteristic of both sugar cane and energy cane. (authors)

  12. Study about the sensibility in vitro of different strains of Vibrio cholera 01 exposed to 60 Co gamma radiation

    International Nuclear Information System (INIS)

    The presence of some microorganisms in food, or the metabolites originated during their own multiplication may bring several diseases to humans: intoxications and food borne infections. Among the agents that may cause those diseases, we find Vibrio cholerae 01. In this experiment, the studies are focused on the radiosensibility in vitro of four strains of V. cholerae 01, exposed to different doses of ionizing radiation of 60 Co. The results are compared with other data related to bacterial food borne diseases, including water. (author)

  13. Improving the Content of Terpenoid Indole Alkaloids in Catharanthus roseus by 60Coγ Radiation%60Coγ射线辐射诱变提高长春花生物碱含量

    Institute of Scientific and Technical Information of China (English)

    赵剑; 李建国

    2009-01-01

    本实验采用长春花种子为材料,用60Coγ射线不同剂量处理,统计处理萌发率和成株率,并观察当代表型变异率和进行回归模型分析.结果分析60Coγ射线辐照长春花种子的半致死剂量平均为174 Gy,照射长春花种子的较适宜剂量为200 Gy.我们观察到长春花M,代生长初期有明显的叶形不对称、叶缘卷曲等致变现象,各种畸形叶出现频率与辐照剂量大小有关.通过辐照,我们得到长春花高杆变异、矮杆变异等变异植株,并利用HPLC检测发现高杆变异株型长春花生物碱含量比未照射的CK多出24%.

  14. The protector role of Mn2+ in Saccharomyces cerevisiae exposed to 60Co gamma radiation

    International Nuclear Information System (INIS)

    The radiation is present on all over the world and can be classified, according to the effect that produces on matter, as non-ionizing and ionizing. The ionizing radiations transfer a sufficient amount of energy to the molecule and charged atoms called ions can be formatted. The biological organisms have approximately 70% of water and when submit to the incident radiation occur the radiolysis of water. Water radiolysis produces highly reactive oxygen species (ROS) known as free radicals. The ROS can interact with all important biomolecules like lipid, protein and DNA. This interaction can cause the loss of functions of theses molecules and thus lead to cell death. The manganese is a heavy metal that at low concentrations is essential for the biological system, since it is cofactor for action of several enzymes. At high concentrations the manganese is toxic to the organisms. Recently was observed that manganese can have a protector role against oxidative stress. In this work, it was studied the anti-oxidant role of manganese in cells submitted to gamma irradiation. It was performed the determination of two oxidative stress indicators: lipid peroxidation and determination of thiol group, the results presented suggest that the manganese had protective role against lipid damage and in this way, has an antioxidant role. (author)

  15. Dose in sensitive organs during the prostate treatment with a 60Co unit

    International Nuclear Information System (INIS)

    The absorbed dose by the bladder, the rectum and the gland thyroid was measured during a treatment applied for prostate cancer by means of a Cobalt 60 unit. The dose was measured using thermoluminescent dosimeters of the type TLD 100, with the values of the absorbed the values of the effective dose were calculated and was determined the probability of the development of a secondary cancer. Because these measurements cannot be made -in vivo- a phantom or mannequin was built with water that represents the hip and part of the torso of the human body and to represent the neck was used polyethylene. The study was carried out in the Instituto Zacatecano del Tumor that has a -cobalt bomb- which is used to treat oncology patients, during the phantom irradiation a dose of 200 c Gy was applied of this dose the bladder received 96.7%, the rectum 100.8% and the gland thyroid 0.3%. The dose received by the rectum and the bladder is due to the therapeutic beam while the dose received by the thyroid is due to the dispersed radiation by the phantom. The probability that in these organs a new neoplasm is developed is of 0.033% for the bladder, 0.157% for the rectum and 7.8 x 10-5% for the thyroid case. (Author)

  16. Precision measurements of the $^{60}$Co $\\beta$-asymmetry parameter in search for tensor currents in weak interactions

    CERN Document Server

    Wauters, F; Zákoucký, D; Beck, M; Breitenfeldt, M; De Leebeeck, V; Golovko, V V; Kozlov, V Yu; Phalet, T; Roccia, S; Soti, G; Tandecki, M; Towner, I S; Traykov, E; Van Gorp, S; Severijns, N

    2010-01-01

    The $\\beta$-asymmetry parameter $\\widetilde{A}$ for the Gamow-Teller decay of $^{60}$Co was measured by polarizing the radioactive nuclei with the brute force low-temperature nuclear-orientation method. The $^{60}$Co activity was cooled down to milliKelvin temperatures in a $^3$He-$^4$He dilution refrigerator in an external 13 T magnetic field. The $\\beta$ particles were observed by a 500 ${\\mu}m$ thick Si PIN diode operating at a temperature of about 10 K in a magnetic field of 0.6 T. Extensive GEANT4 Monte-Carlo simulations were performed to gain control over the systematic effects. Our result, $\\widetilde{A} = -1.014(12)_{stat}(16)_{syst}$, is in agreement with the Standard-Model value of $-0.987(9)$, which includes recoil-order corrections that were addressed for the first time for this isotope. Further, it enables limits to be placed on possible tensor-type charged weak currents as well as other physics beyond the Standard Model.

  17. Determination of distribution coefficients for 134 Cs, 60 Co and 234 Th radionuclides in Pinheiro river sediment

    International Nuclear Information System (INIS)

    The distribution coefficients (K α) were determined in order to foresee the fate of the radionuclides discharged to the environment. Based upon the source-term released by IPEN's facilities in Pinheiros River during the year of 1988, three radionuclides were chosen as being the more critical, according to the radiation protection standards: 137 Cs, 60 Co and 232 Th. Their K α were determined experimentally in laboratory by using the corresponding radioactive tracers 134 Cs, 60 Co and 234 Th. Three different experimental methodologies were used: the static method, the shaken method and the dynamic method. The parameters studied were the effects of pH, aerobic condition and time of contact. The results obtained experimentally for the Kds confirm the predictions that: the cesium is slowly retained by the sediment along the Pinheiros River, the cobalt is an unstable element, therefore its retention by the sediment is affected by variations in the pH values, and finally, the thorium is almost completely retained in the vicinity of the discharge point. (author)

  18. The effects of exposure of {sup 60}Co on the oxidant/antioxidant status among radiation victims

    Energy Technology Data Exchange (ETDEWEB)

    Demir, Mustafa E-mail: mmdemir@e-kolay.net; Konukoglu, Dildar; Kabasakal, Levent; Yelke, Hakan Kadir; Ergen, Kadir; Ahmed, Sabbir

    2002-07-01

    This retrospective study has been performed with radiation victims who were accidentally exposed to a {sup 60}Co source and its release into the environment. The aim of the study was to assess the effects of elevated radiation exposures on plasma level, on erythrocyte thio barbituric acid reactive substance (TBARS) level and on erythrocyte glutathione (GSH) levels. Patients were treated in different hospitals with different symptoms such as nausea, vomiting, dizziness, along with severe anemia in some patients. Blood samples were collected 3-5 days following the radiation accident. Increases in plasma (6.25{+-}0.90 nmol ml{sup -1}) and erythrocyte TBARS levels (330.5{+-}30.5 {mu}mol gHb{sup -1}) were found in comparison to a healthy group (3.72{+-}0.68 nmol ml{sup -1} and 150.7{+-}20.5 {mu}mol gHb{sup -1}, respectively) at a significant level (p<0.001). Erythrocyte GSH levels (5.2{+-}0.30 {mu}mol gHb{sup -1}) were found to be decreased among the victims (healthy group: 10.2{+-}0.7 {mu}mol gHb{sup -1}) at the same significance level (p<0.001). These observations confirm a significant change induced by radiation in the oxidant/antioxidant status among the victims. It is suggested here that antioxidant supplementation therapy might be effective in preventing the harmful effects of {sup 60}Co radiation among radiation victims.

  19. Development and characterization of a graphite-walled ionization chamber as a reference dosimeter for 60Co beams

    Science.gov (United States)

    Perini, Ana P.; Neves, Lucio P.; Caldas, Linda V. E.

    2014-11-01

    A graphite-walled ionization chamber with a sensitive volume of 6.4 cm3 was developed at the Calibration Laboratory of IPEN (LCI) to determine the air kerma rate of a 60Co source. This new prototype was developed to be a simple chamber, without significant nongraphite components and with a simple set-up, which allows the determination of its various required correction factors by Monte Carlo simulations. This new ionization chamber was characterized according to the IEC 60731 standard, and all results were obtained within its limits. Furthermore, Monte Carlo simulations were undertaken to obtain the correction factors involved with the air kerma determination. The air kerma rate obtained with the graphite-walled ionization chamber was compared with that from the reference dosimeter at the LCI, a PTW ionization chamber (model TN30002). The results obtained showed good agreement within the statistical uncertainties. A graphite ionization chamber was assembled and characterized as a reference dosimeter. The characterization test results were within recommended limits. Monte Carlo simulations were undertaken to obtain the correction factors. The air kerma rate of a 60Co source was obtained with satisfactory results.

  20. Laboratory studies of the diffusive transport of {sup 137}Cs and {sup 60}Co through potential waste repository soils

    Energy Technology Data Exchange (ETDEWEB)

    Itakura, Takashi [Australian Nuclear Science and Technology Organisation, Menai, NSW (Australia); Airey, David W., E-mail: david.airey@sydney.edu.a [School of Civil Engineering, University of Sydney, Sydney, NSW 2006 (Australia); Leo, Chin Jian [School of Engineering and Industrial Design, University of Western Sydney, Penrith, NSW (Australia); Payne, Timothy; McOrist, Gordon D. [Australian Nuclear Science and Technology Organisation, Menai, NSW (Australia)

    2010-09-15

    Tests using reconstituted samples have been performed to assess the diffusive transport of {sup 137}Cs and {sup 60}Co through natural regolith materials from a region in South Australia being considered for a radioactive waste repository. A double diffusion cell apparatus made of polycarbonate resin was developed to estimate the effective diffusion (D{sub e}) and sorption coefficients (K{sub d}) that allowed large withdrawals from the source and collector cells and has enabled tests with low concentrations of radioactivity. An alternative to porous stainless steel filter plates has also been used to reduce uncertainty in test interpretation. Analysis of the transient data used a staged method of the Laplace transform to take into consideration the volume of the samples withdrawn from the apparatus during testing. At test completion samples were cut into slices and analysed for radionuclide concentration. Data obtained from the sliced samples confirmed that both numerical and experimental data produced acceptable mass balance. The D{sub e} values obtained in this study were of the order of 10{sup -6} cm{sup 2} s{sup -1} for both species, higher than previously published data. The K{sub d} values from the diffusion and batch sorption tests were in reasonable agreement for {sup 137}Cs, but an order of magnitude different for {sup 60}Co. The sorption of the latter radionuclide was strongly pH dependent, and this dependency during diffusion tests would benefit from further investigation.

  1. Whey powder sterilization by ionizing irradiation

    International Nuclear Information System (INIS)

    Whey powder was sterilized by gamma waves application. As a source of irridiation isotope 60Co was used in Institute of Nuclear sciences Boris Kidrich, Vincha-Belgrade (Yugoslavia). The applied doses were: a, b, c, d, and e Kgy. The dose d was radappertization. After whey powder irradiation no adverse changes of organoleptic properties were noticed

  2. 60Co-γ射线辐照育种马铃薯新品系鉴定试验%New Strains Identification of Potatos Breeded by 60Co-γRay Radiation

    Institute of Scientific and Technical Information of China (English)

    邓宽平; 丁海兵; 罗治霞; 夏锦慧

    2008-01-01

    对5个60Co-γ射线辐照育种马铃薯新品系进行了比较鉴定试验.结果表明:F8-02比对照品种费乌瑞它小区增产17.9 kg,增产率为79.2%,产量居第1位,F8-01比对照增产5.8 kg,增产率为25.7%,产量居第2位.小区产量居前两位的品系均比对照抗马铃薯晚疫病,且综合性状较好.

  3. Mutation effect of radiation of 60 Co γ ray on variant strain of Pleurotus ostreatus%60Coγ射线对侧耳属变异株的诱变效应

    Institute of Scientific and Technical Information of China (English)

    陈再鸣; 曹若彬; 黄玲; 董荷玲

    2001-01-01

    应用60Co γ射线对侧耳属变异株的双核菌丝体进行辐射诱变处理,并对其再生菌落进行菌丝形态学、细胞学、生理生化鉴定。结果发现,诱变株菌落形态发生明显变化,气生菌丝减少,生长过程减慢,细胞直径变大,分枝增多,与出发菌株拮抗线明显,EST,POD同工酶显示其酶谱差异显著。结实性研究表明,诱变株较出发株子实体分化正常,产量提高,抗性增强,品质良好。显示60 Co γ射线辐射诱变是改良侧耳属菌株的有效途径。%The dicaryotic hyphae of the variant strain of P.ostreatus was treated by radiation of 60 Co γ ray. Mycelial morpholagical,cytological and physiological-biochemical characteristics of its mutant strain were studied.The results showed that the mutant strain had less aerial mycelium.Lower colony growth rate,bigger mycelium diameter,more branch on vegetative growth phase and had more fruitbody formation,higher yield,better quality on reproductive growth phase than the initial strain.Clear ant-agnistic line and significant differences of isoenzyme pattern of EST and POD existed between the mutant and initial strains.It showed that induced mutation from dicaryotic hyphae by 60 Co γ ray was an effective means of gaining excellent rejuvenation strain of P.ostreatus.

  4. Action of Bothrops moojeni venom and its L-amino acid oxidase fraction, treated with 60Co gamma rays, in Leishmania spp

    International Nuclear Information System (INIS)

    Bothrops moojeni venom showed an anti leishmania activity in vitro, as determined by a cell viability assay using the reduction of MTT. After venom purification, by chromatography techniques, the fractions with anti leishmania and L-amino acid oxidase activities, eluted in the same positions. The molecular weight of the enzyme was estimated to be 140 kDa by molecular exclusion chromatography, and 69 kDa, by SDS-PAGE, migrating as a single band, with an isoelectric point of 4.8 as determined by isoelectric focusing. The purified LAO from B. moojeni venom, 135-fold more active than crude venom, showed homo dimeric constitution, and was active against Leishmania spp from the New World, with an effective concentration against L(L). amazonensis of 1.80 μg/ml (EC50), L.(V.) panamensis (0.78 |μg/ml) and L.(L.) chagasi (0.63 (μg/ml). Ultrastructural studies of promastigotes affected by LAO demonstrated cell death, with edema in several organelles such as mitochondria and nuclear membrane, before cell disruption and necrosis. The action of LAO was demonstrated to be hydrogen peroxide-dependent. Studies with LLCMK-2 cells, treated with LAO, showed a toxic effect, with an EC50 of 11|μg/ml. Irradiation of LAO with 60Co gamma rays, did not affect its whole oxidative activity, neither detoxified the enzyme. Amastigotes treated with LAO were not affected by its hydrogen peroxide, otherwise, the exogenous product, killed amastigotes with an EC50 of 0.67mM. These data could be of help in the development of alternative therapeutic approaches to the treatment of leishmaniasis. (author)

  5. Fluorescence in situ hybridization detection of chromosome translocations induced by 60Co γ-rays in human lymphocytes dose-response curve and persistence%用荧光原位杂交技术建立60Coγ射线诱发人外周血淋巴细胞染色体易位率剂量效应曲线及易位的持续性研究

    Institute of Scientific and Technical Information of China (English)

    王晓琳; 李进; 王知权

    2001-01-01

    Objective To study dose-response relationship for chromosome translocation and its persistence measured in human lymphocytes exposed to 60Co γ-rays.Methods The chromosome translocations in human peripheral lymphocytes were detected by fluorescence in situ hybridization using 4# and 7# combination of composite whole chromosome-specific DNA probes and Giemsa stain.Results The dose-response relationship for chromosome translocation induced by 60Co γ-rays in vitro could be described by the function:Y=0.0030+0.0134D+0.0165D2.The frequencies of chromosome translocations induced by 0.5 and 2 Gy γ-irradiation did not diminish over time,so it exhibited excellent persistence.Conclusions The results indicate that retrospective dose-reconstruction can be accomplished using chromosome translocation frequency.%目的研究不同剂量60Coγ射线诱发人外周血淋巴细胞染色体易位率和辐射剂量间的剂量效应关系以及观察易位率在同一剂量、不同时相点的变化。方法采用荧光原位杂交技术和连续Giemsa法,用4号和7号全染色体探针分析0、0.25、0.5、0.75、1和2 Gy 60Coγ射线离体照射诱发人外周血淋巴细胞染色体易位率,同时对两个剂量点进行52和72 h培养,比较其易位率。结果易位率和辐射剂量间的量效关系可以用一个二次多项式方程Y=0.0030+0.0134D+0.0165D2来描述;易位率在52和72 h培养时差异无显著性。结论易位率和辐射剂量间存在良好的量效关系,可作为进行早先照射剂量重建的理论依据。

  6. Gamma ({sup 60}CO) radiation effects on arcelin protein and evaluation of bean lineages against Acanthoscelides obtectus (Say) and Zabrotes subfasciatus (Boh.) (Col.: Bruchidae);Efeito da radiacao gama ({sup 60}CO) sobre a proteina arcelina e avaliacao de linhagens de feijoeiro a Acanthoscelides obtectus (Say) and Zabrotes subfasciatus (Boh.) (Col.: Bruchidae)

    Energy Technology Data Exchange (ETDEWEB)

    Botelho, Ana Claudia Girardo

    2006-07-01

    The resistance of arcelin carrying seeds of bean lineages (Phaseolus vulgaris L.) against the bean weevils Acanthoscelides obtectus (Say, 1831) and Zabrotes subfasciatus (Bohemann, 1833) (Coleoptera: Bruchidae), and the influence of gamma radiation ({sup 60}CO) on the manifestation of arcelin resistance to Z. subfasciatus were verified. Laboratorial tests, in choice and non-choice tests, with wild specimens carrying Arc-1, Arc-2, Arc-3, Arc-4, Raz-56 and Raz-59 (with Arc-5 alleles) and commercial lineages as control IAC - Carioca and IAC - Arua were conducted. Statistical design was completely randomized, with five repetitions, with 10 g of grains from each lineage samples by portion. Attractiveness, oviposition, emergence, mortality, adults' weigh and longevity, developing period, sexual rate, seeds' weigh loss, infestation and fecundity (Z. subfasciatus) were observed. Gamma radiation doses irradiations, in general, haven't affected the resistance manifestation of lineages carrying arcelin protein variants against the Z. subfasciatus bean weevil, thus, joint application use of both control methods can be recommended. Raz-56 lineage showed high resistance of the antibiosis types and non-preference for oviposition and feeding to Z. subfasciatus, while Raz-59 showed antibiosis and non-preference for feeding, and both (Raz-56 and Raz-59) showed intermediate resistance to A. obtectus, against which lineage Arc-2 was the most harmful to its development, expressing non-preference to feeding and/or antibiosis. (author)

  7. Assessing deposition levels of {sup 55}Fe, {sup 60}Co and {sup 63}Ni in the Ignalina NPP environment

    Energy Technology Data Exchange (ETDEWEB)

    Gudelis, A., E-mail: gudelis@ktl.mii.l [Nuclear and Environmental Radioactivity Research Laboratory, Institute of Physics, Savanoriu Avenue, 231, LT-02300 Vilnius (Lithuania); Druteikiene, R.; Luksiene, B.; Gvozdaite, R. [Nuclear and Environmental Radioactivity Research Laboratory, Institute of Physics, Savanoriu Avenue, 231, LT-02300 Vilnius (Lithuania); Nielsen, S.P.; Hou, X. [Riso National Laboratory for Sustainable Energy, Technical University of Denmark, P.O. Box 49, DK-4000, Roskilde (Denmark); Mazeika, J.; Petrosius, R. [Radioisotope Laboratory, Institute of Geology and Geography, Sevcenkos Street, 13, LT-03223 Vilnius (Lithuania)

    2010-06-15

    Two RBMK-1500 reactor units operated in Lithuania in the 1987-2004 period (one of them was stopped for decommissioning in 2004). This study presents a preliminary investigation of surface deposition density levels of {sup 55}Fe and {sup 63}Ni in moss samples collected in the close vicinity of the Ignalina NPP. Non-destructive analysis by the HPGe gamma-spectrometry was followed by radiochemical separation. Radiochemical analysis was based on anion-exchange and extraction chromatography. {sup 55}Fe and {sup 63}Ni activities were measured by liquid scintillation counting (LSC). The results indicate that the deposition values of {sup 55}Fe are generally higher than those of {sup 60}Co and {sup 63}Ni.

  8. Direct MC conversion of absorbed dose to graphite to absorbed dose to water for 60Co radiation.

    Science.gov (United States)

    Lye, J E; Butler, D J; Franich, R D; Harty, P D; Oliver, C P; Ramanathan, G; Webb, D V; Wright, T

    2013-06-01

    The ARPANSA calibration service for (60)Co gamma rays is based on a primary standard graphite calorimeter that measures absorbed dose to graphite. Measurements with the calorimeter are converted to the absorbed dose to water using the calculation of the ratio of the absorbed dose in the calorimeter to the absorbed dose in a water phantom. ARPANSA has recently changed the basis of this calculation from a photon fluence scaling method to a direct Monte Carlo (MC) calculation. The MC conversion uses an EGSnrc model of the cobalt source that has been validated against water tank and graphite phantom measurements, a step that is required to quantify uncertainties in the underlying interaction coefficients in the MC code. A comparison with the Bureau International des Poids et Mesures (BIPM) as part of the key comparison BIPM.RI(I)-K4 showed an agreement of 0.9973 (53). PMID:23152147

  9. Chromosome damage and clinical manifestation in a fetus and the mother after accidental 60Co exposure in Xinzhou

    International Nuclear Information System (INIS)

    The authors present the clinical effect and chromosome damage sustained by a fetus and the four months pregnant mother in an accidental 60Co exposure in November of 1992 in Xinzhou, Shanxi Province. The mother suffered from a moderate acute radiation sickness with ratardation of fetal development. After delivery, the infant's body length, body weight and head circumference were all lowered by three percentiles compared with the normals. Four months after the exposure, the assay of the mother's peripheral lymphocytes showed a chromosome aberration rate of 36%, while concomitant examination of the baby failed to reveal any chromosome abnormality although the sister chromatid exchange rate was remarkably higher than that of the mother and the normal control

  10. A new standard cylindrical graphite-walled ionization chamber for dosimetry in 60Co beams at calibration laboratories

    Science.gov (United States)

    Neves, Lucio P.; Perini, Ana P.; Caldas, Linda V. E.

    2014-11-01

    60Co sources are used mostly at dosimetry laboratories for calibration of ionization chambers utilized for radiotherapy dosimetry, mainly in those laboratories where there is no linear accelerator available. In this work, a new cylindrical ionization chamber was developed and characterized to be used as a reference dosimeter at the Calibration Laboratory of the IPEN. The characterization tests were performed according to the IEC 60731 standard, and all tests presented results within its recommended limits. Furthermore, the correction factors for the wall, stem, central collecting electrode, nonaxial uniformity and the mass-energy absorption coefficient were determined using the EGSnrc Monte Carlo code. The air kerma rate determined with this new dosimeter was compared to the one obtained with the IPEN standard, presenting a difference of 1.5%. Therefore, the new ionization chamber prototype developed and characterized in this work presents potential use as a primary standard dosimeter at radiation metrology laboratories.

  11. Determination of 137Cs and 60Co pollution in the area of the Laguna Verde Nuclear Power Plant, Mexico

    International Nuclear Information System (INIS)

    The project 'Radiological Analysis of Environmental Samples in the Gulf of Mexico and the coast of Quintana Roo', had the aim of identifying and quantifying anthropogenic radionuclides in environmental samples consisting of silt, sand and sea water. This paper presents the results of the radiological analysis of these samples, which was made in the multichannel system for gamma spectrometry with hyper-pure germanium detector in the Laboratory of Radiological Analysis of Environmental Samples, located at the Physics Department, Faculty of Sciences, of the Autonomous National University of Mexico (UNAM). The sampled points are along the coast of the contiguous states of Tamaulipas, Veracruz, Tabasco, Campeche, Yucatan and Quintana Roo. This paper presents the qualitative and quantitative concentrations of the main identified anthropogenic radionuclides 60Co and 137Cs. (authors)

  12. Determination of 137Cs and 60Co pollution in the area of the Laguna Verde Nuclear Power Plant, Mexico.

    Science.gov (United States)

    Salas Mar, Bernardo

    2015-11-01

    The project 'Radiological Analysis of Environmental Samples in the Gulf of Mexico and the coast of Quintana Roo', had the aim of identifying and quantifying anthropogenic radionuclides in environmental samples consisting of silt, sand and sea water. This paper presents the results of the radiological analysis of these samples, which was made in the multichannel system for gamma spectrometry with hyperpure germanium detector in the Laboratory of Radiological Analysis of Environmental Samples, located at the Physics Department, Faculty of Sciences, of the Autonomous National University of Mexico (UNAM). The sampled points are along the coast of the contiguous states of Tamaulipas, Veracruz, Tabasco, Campeche, Yucatan and Quintana Roo. This paper presents the qualitative and quantitative concentrations of the main identified anthropogenic radionuclides (60)Co and (137)Cs. PMID:25944960

  13. Comparison of the standards of air kerma of the OMH and the BIPM for 60Co gamma radiation

    International Nuclear Information System (INIS)

    A direct comparison between the standards for air kerma of the Orszagos Meresugyi Hivatal (OMH) and of the Bureau International des Poids et Mesures (BIPM) has been carried out in the 60Co radiation beams of the BIPM. The result, expressed as a ratio of the OMH and the BIPM standards for air kerma, indicates a relative difference of 10.9 x 10-3 with a combined standard uncertainty of 2.2 x 10-3. This new result agrees at the level of 0.4 x 10-3 with the earlier direct comparisons performed in 1986 and 1994, as modified in 2001 by the application of wall and axial non-uniformity correction factors, calculated for the OMH standards using the Monte Carlo method. (authors)

  14. An investigation on the radiation sensitivity of DNA conformations to 60Co gamma rays by using Geant4 toolkit

    International Nuclear Information System (INIS)

    To investigate the impact of conformational properties of genetic material of living cells on radiation-induced DNA damage, single strand breaks (SSB), double strand breaks (DSB) and some microdosimetric quantities of A, B and Z-DNA conformations caused by 60Co gamma rays, have been calculated. Based on a previous B-DNA geometrical model, models of A and Z forms have been developed. Simple 34 base pairs segments of each model repeated in high number and secondary electron spectrum of 60Co gamma rays have been simulated in a volume of a typical animal cell nucleus. All simulations in this study have been performed by using the Geant4 (GEometry ANd Tracking 4)-DNA extension of the Geant4 toolkit. The results showed that, B-DNA has the lowest yield of simple strand breaks with 2.23 × 10−10 Gy−1 Da−1 and 1.0 × 10−11 Gy−1 Da−1 for the SSB and DSB damage yield, respectively. The A-DNA has the highest SSB yield with 3.59 × 10−10 Gy−1 Da−1 and the Z-DNA has the highest DSB yields with 1.8 × 10−11 Gy−1 Da−1. It has been concluded that there is a direct correlation between the hit probability, mean specific imparted energy and SSB yield in each model of DNA. Moreover, there is a direct correlation between the DSB yield and both the mean lineal energy and topological characteristics of each model

  15. An investigation on the radiation sensitivity of DNA conformations to {sup 60}Co gamma rays by using Geant4 toolkit

    Energy Technology Data Exchange (ETDEWEB)

    Semsarha, F. [Institute of Biochemistry and Biophysics (IBB), University of Tehran, P.O. Box 13145-1384, Tehran (Iran, Islamic Republic of); Goliaei, B., E-mail: goliaei@ibb.ut.ac.ir [Institute of Biochemistry and Biophysics (IBB), University of Tehran, P.O. Box 13145-1384, Tehran (Iran, Islamic Republic of); Raisali, G.; Khalafi, H. [Radiation Applications Research School, Nuclear Science and Technology Research Institute, P.O. Box 11365-3486, Tehran (Iran, Islamic Republic of); Mirzakhanian, L. [Medical Physics Unit, McGill University, 1650 Cedar Avenue, Montreal, Quebec (Canada)

    2014-03-15

    To investigate the impact of conformational properties of genetic material of living cells on radiation-induced DNA damage, single strand breaks (SSB), double strand breaks (DSB) and some microdosimetric quantities of A, B and Z-DNA conformations caused by {sup 60}Co gamma rays, have been calculated. Based on a previous B-DNA geometrical model, models of A and Z forms have been developed. Simple 34 base pairs segments of each model repeated in high number and secondary electron spectrum of {sup 60}Co gamma rays have been simulated in a volume of a typical animal cell nucleus. All simulations in this study have been performed by using the Geant4 (GEometry ANd Tracking 4)-DNA extension of the Geant4 toolkit. The results showed that, B-DNA has the lowest yield of simple strand breaks with 2.23 × 10{sup −10} Gy{sup −1} Da{sup −1} and 1.0 × 10{sup −11} Gy{sup −1} Da{sup −1} for the SSB and DSB damage yield, respectively. The A-DNA has the highest SSB yield with 3.59 × 10{sup −10} Gy{sup −1} Da{sup −1} and the Z-DNA has the highest DSB yields with 1.8 × 10{sup −11} Gy{sup −1} Da{sup −1}. It has been concluded that there is a direct correlation between the hit probability, mean specific imparted energy and SSB yield in each model of DNA. Moreover, there is a direct correlation between the DSB yield and both the mean lineal energy and topological characteristics of each model.

  16. Displacement correction factor versus effective point of measurement in depth dose curve measurements at 60Co gamma rays

    International Nuclear Information System (INIS)

    The discrepancies in data sets of values of the Displacement Factor pd recommended by different codes of practices for calibration purpose still demand further investigation to clarify this point. In this paper, we propose an experimental method to determine the displacement factor for cylindrical ionization chambers (thimble chambers) in photon beams. Measurements of pd for several depths were performed for 60Co gamma rays. From these results we calculated the shift of the effective point of measurement (z-zeff) for different depths. The results obtained in this work shown: (a) there is no significant change in pd from 2 cm to 17 cm of depth in water; (b) the value of pd for a ion-chamber Farmer type (inner radius r = 3.15 cm) is pd 0.988; (c) the shift of the effective point of measurement has a smooth variation with depth; (d) the value of (z-zeff) at the recommended calibration depth for 60Co beams (5 cm) is 0.6r (with r: inner radius of the chamber). The result (b) confirms the value of pd suggested by the SEFM and NACP protocols and differs with that of the AAPM. The value obtained for (z - zeff) (d) is very closed to that recommended by the IAEA TRS-277. Finally, the results (a) and (c) suggest that it should be preferable to use the displacement factor instead of effective point of measurement to perform measurements of depth dose curves, since the use of zeff should take into account its dependence on depth. (author). 7 refs, 4 figs

  17. Study of the action of {sup 60}Co gamma radiation on Salmonella poona, Escherichia coli and Alicyclobacillus acidoterrestris in mango pulp; Estudo da acao da radiacao gama de {sup 60}Co sobre Salmonella poona, Escherichia coli e Alicyclobacillus acidoterrestris em polpa de manga congelada

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Marco Antonio dos Santos

    2009-07-01

    The application of non-thermal treatments has proven effective in inhibiting bacteria such as Salmonella and Escherichia coli. Mango is a fruit of national consumption with a great exportation potential. Meanwhile, outbreaks of food borne disease related to mango consumption caused mistrust on the degree of food security offered by the product. The objective of this work was to establish the radioresistance of bacteria Escherichia coli, Salmonella Poona and Alicyclobacillus acidoterrestris on the mango pulp by the calculation of the D10 values and to know the radiation effect on the sensory characteristics of the fruit pulp. The microbiological profile of frozen mango pulp available at the local market was also established using conventional methods of plating and Most Probable Number (MPN). The pulps experimentally inoculated with the bacteria listed above were irradiated with doses of 0, 1, 2, 3, 4 and 5kGy in a {sup 60}Co source. The sensory analysis was performed using a dose of 5 kGy, using the triangular test and the test of acceptance with hedonic scale. The results of this study show that the quality of mango pulp sell in the local market is not satisfactory in accordance with the standards established by the Brazilian law and the literature, showing the need of using other tools to achieve acceptable levels of quality. The D10 values obtained are in the range of 1.01 and 1.09kGy for E. coli ATCC 8739, 0.60 and 0.98kGy for S. poona and 0.72 e 0.88kGy for A. acidoterrestris respectively. The triangular test showed that a 5kGy radiation dose changed the sensory characteristics of mango pulp. Nevertheless, sensory analysis of a food product prepared with the irradiated pulp obtained good acceptance in the attributes of global appearance, flavor and aroma. (author)

  18. Study of the action of {sup 60}Co gamma radiation on Salmonella poona, Escherichia coli and Alicyclobacillus acidoterrestris in mango pulp;Estudo da acao da radiacao gama de {sup 60}Co sobre Salmonella poona, Escherichia coli and Alicyclobacillus acidoterrestris em polpa de manga congelada

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Marco Antonio dos Santos

    2009-07-01

    The application of non-thermal treatments has proven effective in inhibiting bacteria such as Salmonella and Escherichia coli. Mango is a fruit of national consumption with a great exportation potential. Meanwhile, outbreaks of foodborne disease related to mango consumption caused mistrust on the degree of food security offered by the product. The objective of this work was to establish the radioresistance of bacteria Escherichia coli, Salmonella Poona and Alicyclobacillus acidoterrestris on the mango pulp by the calculation of the D10 values and to know the radiation effect on the sensory characteristics of the fruit pulp. The microbiological profile of frozen mango pulp available at the local market was also established using conventional methods of plating and Most Probable Number (MPN). The pulps experimentally inoculated with the bacteria listed above were irradiated with doses of 0, 1, 2, 3, 4 and 5 kGy in a {sup 60}Co source. The sensory analysis was performed using a dose of 5 kGy, using the triangular test and the test of acceptance with hedonic scale. The results of this study show that the quality of mango pulp sell in the local market is not satisfactory in accordance with the standards established by the Brazilian law and the literature, showing the need of using other tools to achieve acceptable levels of quality. The D10 values obtained are in the range of 1.01 and 1.09 kGy for E. coli ATCC 8739, 0.60 and 0.98 kGy for S. poona and 0.72 and 0.88 kGy for A. acidoterrestris respectively. The triangular test showed that a 5 kGy radiation dose changed the sensory characteristics of mango pulp. Nevertheless, sensory analysis of a food product prepared with the irradiated pulp obtained good acceptance in the attributes of global appearance, flavor and aroma. (author)

  19. Influence of temperature decrease and soil drought on the geochemical fractionation of 60Co and 137Cs in fluvisol and cambisol soils

    International Nuclear Information System (INIS)

    Highlights: • Fractionation of 60Co and 137Cs in soils depends on climate conditions. • Freezing or soil drought increase the water-solubility of 60Co in fluvisol soil. • Temperature decrease and soil drought cause an increase of the exchangeable 137Cs. • The impact of climate on 60Co and 137Cs mobility is higher on fluvisol soil. - Abstract: Consideration of the impact of substantial changes in soil temperature or moisture regime on the geochemical forms of radionuclides is important for more accurate assessment of the environmental risk posed by radionuclide migration and potential biological availability, especially in the first months after their release into the environment. This paper presents the results from a study of the influence of cooling, freezing and soil drought on the migration and potential bioavailability of 60Co and 137Cs in two soils (a fluvisol and a cambisol, according to the World Reference Base for Soil Resources/FAO) from Bulgaria. The changes in the geochemical fractionation of 60Co, the exchangeable 137Cs and water-soluble forms of both radionuclides were examined under different storage conditions up to 5 months after their introduction into the soils in solution form. Freezing or soil drought resulted in a significant increase of the water-soluble forms of 60Co in the fluvisol soil, defining higher mobility and potential bioavailability. No influence of the storing conditions on the water-solubility of 60Co in the cambisol soil was established. The cooling, freezing and soil drought caused an increase of the exchangeable 137Cs in both soils

  20. Gamma irradiation effects in W films

    Energy Technology Data Exchange (ETDEWEB)

    Claro, Luiz H. [Instituto de Estudos Avancados - IEAv, Rod. dos Tamoios, km 5,5, CEP: 12228-840, Sao Jose dos Campos, SP (Brazil) and Faculdade de Tecnologia Sao Francisco - FATESF, Av. Siqueira Campos, 1174, CEP: 12207-000, Jacarei (Brazil); Santos, Ingrid A. [Instituto de Estudos Avancados - IEAv, Rod. dos Tamoios, km 5,5, CEP: 12228-840, Sao Jose dos Campos, SP (Brazil); Silva, Cassia F. [Faculdade de Tecnologia Sao Francisco - FATESF, Av. Siqueira Campos, 1174, CEP: 12207-000, Jacarei, SP (Brazil)

    2013-05-06

    Using the van Der Pauw methodology, the surface resistivity of irradiated tungsten films deposited on Silicon substrate was measured. The films were exposed to {gamma} radiation using a isotopic {sup 60}Co source in three irradiation stages attaining 40.35 kGy in total dose. The obtained results for superficial resistivity display a time annealing features and their values are proportional to the total dose.

  1. Final environmental statement concerning rule making. Exemption from licensing requirements for spark-gap irradiators that contain cobalt-60. Docket No. PRM 30-54

    International Nuclear Information System (INIS)

    The potential environmental impacts and adverse environmental effects from distribution, use only in commercial-sized oil burners, and disposal of 6000 spark-gap irradiators per year that contain 60Co are summarized. On the basis of the analysis and evaluation set forth in this statement and after weighing the environmental, economic, technical, and other benefits against environmental costs and after considering available alternatives, it is concluded that the action called for under the National Environmental Policy Act of 1969 (NEPA) and 10 CFR Part 51 is the issuance of an exemption from licensing requirements for spark-gap irradiators that contain 60Co, subject to the following conditions for the protection of the environment: persons who apply 60Co to, or persons who incorporate 60Co into, spark-gap irradiators or persons who import for sale or distribution spark-gap irradiators containing 60Co are not exempt from the requirements for a license; each spark-gap irradiator shall contain no more than 1 μCi of 60Co; and the 60Co shall be applied to the spark-gap irradiators for use in electrically ignited fuel-oil burners having a firing rate of at least 3 gal/h

  2. Biochemical aspects of the immunomodular action in irradiated survival mice with 60C gama irradiation

    International Nuclear Information System (INIS)

    The radioprotective action of Calmetti-Guerin bacillus (BCG), Corynebacterium parvum, Escherichia coli Lipopolysccharides (LPS) and peptone proteose was evaluated. A single injection of the macrophage activiting agents prior to 60Co whole-body irradiation increased the survival rate of mice in the lethal dose range. (L.M.J.)

  3. Simple and Efficient Method for Measuring Anti-Toxoplasma Immunoglobulin Antibodies in Human Sera Using Complement-Mediated Lysis of Transgenic Tachyzoites Expressing β-Galactosidase

    Science.gov (United States)

    Dando, Caroline; Gabriel, Katie E.; Remington, Jack S.; Parmley, Stephen F.

    2001-01-01

    A simple and efficient method using transgenic Toxoplasma gondii tachyzoites expressing β-galactosidase was developed for detection of specific antibodies against the parasite in sera of patients. The titers obtained with the new test were similar to those obtained with the Sabin-Feldman dye test run in parallel. Although significant changes in endpoint titers were not observed when sera drawn sequentially at 2- to 3-week intervals were tested with both procedures, apparent differences in antibody affinity were observed with the new test which were not perceptible with the Sabin-Feldman dye test. Like the Sabin-Feldman dye test, the new test is based on complement lysis of tachyzoites, but it is much easier to perform and the reaction is read colorimetrically instead of visually. PMID:11376045

  4. Apoptotic activity and anti-Toxoplasma effects of artemether on the tachyzoites and experimental infected Vero and J774 cell lines by Toxoplasma gondii

    OpenAIRE

    Hajar Mikaeiloo; Fatemeh Ghaffarifar; Abdolhossein Dalimi; Zohreh Sharifi; Zuhair Mohammad Hassan

    2016-01-01

    Objectives: Drugs used for toxoplasmosis have limited efficacy and also severe side effects. A new drug with good efficacy and limited side effects is need of the hour. We studied the effects of artemether on Toxoplasma gondii in vitro conditions. Materials and Methods: Artemether (methyl-ether-qinghaosu) was tested for tachyzoites, J774, and Vero cell lines infected by T. gondii. For evaluating the effect of drugs on Vero cells infected with T. gondii, we designed two separate experiments; i...

  5. Effect of 60Co-gamma radiation on the random walk error of interferometric fiber optic gyroscopes

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Two 60Co-gamma radiation experiments were launched to explore the radiation effect on optical components and interferometric fiber optic gyroscope (IFOG). In optical components radiation experiment, the result showed that polarization-maintaining (PM) fiber coil loss was the most affected parameter in all the RWC (random walk coefficient) related parameters, compared with the weak sensitivity of other components parameters. In the IFOG radiation experiment, the RWC performance degradation was found to be almost due to an increase of the PM fiber attenuation. Based on the experiment result, a RWC prediction model in radiation, which is obtained by embedding PM fiber loss expression into the RWC model, was built following a power law of dose and dose rate. An IFOG RWC in space radiation environment was predicted from radiation dose and dose rate by the RWC prediction model. This RWC value calculated from test data is fully accorded to the RWC value predicted from radiation dose.

  6. Screening Quality Mutants of Wheat Radiated by 60Co-γ Ray and Molecular Identification%60Co-γ射线诱变小麦品质突变体的筛选及分子标记检测

    Institute of Scientific and Technical Information of China (English)

    来德娥; 赵平; 王敏; 张从宇

    2012-01-01

    为了探索小麦品质性状的物理诱变效应,用300Gy的60Co-γ射线辐射小麦品种河科2号的干种子,对M3代234个株系的蛋白质含量、湿面筋含量、沉降值、硬度和产量性状进行变异分析和SSR分子鉴定,以超过均值±2×标准差确定变异株系.结果表明,在M3代群体中,蛋白质含量有13个株系发生正向变异,5个株系发生负向变异;湿面筋含量有8个株系发生正向变异,3个株系发生负向变异;沉降值有6个株系发生正向变异,3个株系发生负向变异;硬度有2个株系发生正向变异,9个株系发生负向变异;产量有5个株系发生正向变异,4个株系发生负向变异.相关分析表明,M3群体蛋白质含量、湿面筋含量、沉降值呈极显著正相关,表现出平行变异的趋势.初步筛选出蛋白质含量和湿面筋含量符合国家强筋小麦标准(蛋白质含量≥15%,湿面筋含量≥35%)的株系8个.经SSR分子标记检测,与控制小麦蛋白质含量的基因存在连锁的2个特异引物Xbarc164和Xgwm161在以上8个变异株系都呈现多态性标记,表明这些株系与蛋白质含量相关的基因可能发生了变异.%To clarify the mutagenic effects of radiation to quality characters of wheat, dry seeds of wheat variety Heke 2 were radiated by 60Co-γ ray with the dose of 300 Gy. The genetic variation of 234 M3 lines on protein content,wet gluten content,sedimentation value,hardness and yield were analyzed,and the mutants were identified by molecular marker. Depending on the criterion of over mean ±2 s, 13 positive mutant lines and 5 negative mutant lines in protein content,8 positive mutant lines and 3 negative mutant lines in wet gluten content, 6 positive mutant lines and 3 negative mutant lines in sedimentation value,2 positive mutant lines and 9 negative mutant lines in hardness,and 5 positive mutant lines and 4 negative mutant lines in yield were identified from 234 M3 lines, respectively. Significant

  7. Changes of expression of miRNA induced by 60Coγ radiation in mice and its significance%60Coγ辐射致小鼠血液中miRNA表达改变及意义

    Institute of Scientific and Technical Information of China (English)

    李刚强; 焦成文

    2016-01-01

    目的 探讨60Coγ射线辐射损伤对小鼠血液miRNA的影响及意义.方法 SPF级C57BL/6J小鼠25只随机分为对照组、0.5 Gy照射组、2 Gy照射组、6 Gy照射组和10 Gy照射组,每组5只.0.5 Gy照射组、2 Gy照射组、6 Gy照射组和10 Gy照射组分别采用60Coγ射线0.5、2.0、6.0、10.0 Gy剂量进行射线照射,剂量率为10.0 Gy/h;对照组不进行射线照射.照射后6、24 h各组检测外周血白细胞计数,应用Agilent microRNA生物芯片筛选小鼠血液中差异表达miRNA.结果 照射6h后,0.5 Gy照射组差异表达miRNA 11个,2 Gy照射组16个,6 Gy照射组12个,10 Gy照射组28个,对照组0个;照射24 h后,0.5 Gy组差异表达miRNA 32个,2 Gy照射组36个,6 Gy照射组30个,10 Gy照射组26个,对照组0个;照射6、24 h时各剂量照射组miRNA差异表达均高于对照组(P<0.05);10 Gy照射组照射6h时miRNA差异表达高于0.5、2、6 Gy照射组(P<0.05),照射24h时与0.5、2、6 Gy照射组比较差异无统计学意义(P>0.05);筛选27个miRNA组成的表达谱,对辐射损伤剂量和时间判断的准确率、敏感性和特异性均>90%;照射6、24 h后,6 Gy照射组和10 Gy照射组外周血白细胞计数均明显低于对照组(P<0.05),且照射24 h低于6 h(P<0.05);照射6、24 h时,10 Gy照射组外周血白细胞计数均明显低于0.5、2、6 Gy照射组(P<0.05).结论 miRNA在血浆中差异表达与辐射损伤有关,有可能成为辐射损伤的新血液标志物.

  8. Effects of seed treatment with 60Co gamma rays and micronutrients on germination and growth of corn seedlings

    International Nuclear Information System (INIS)

    Gamma irradiation and soaking in solutions of some micronutrient elements, as presowing treatments of corn seeds, towards improving the germination of seeds, and increasing the growth of seedlings were investigated. The seeds were exposed to 12 irradiation doses of gamma rays ranging from 250-8000R. It was found that stimulatory effects on the germination percentage and capacity of seeds as well as the height and the dry weight of seedlings were exerted only by the low irradiation doses from 500-1000 R. Soaking cornseeds, before sowing, in any of the 4 concentrations ranging from 250-1000 ppm of molybdenum, manganese and zinc indicated that molybdenum treatment increased plant height the dry weight of seedlings; 500 ppm molybdenum gave the best effect. Irradiation of 500 ppm molybdenum soaked seeds with low doses of gamma rays stimulated the germination process and early growth of seedlings, with the 500 R dose being most effective. (author)

  9. 60Co-γ射线辐射对胭脂虫Dactylopius coccus Costa生物学特征的影响%The Research on Biological Characteristics of Cochineal (Dactylopius coccus Costa) Radiated by 60Co-γ Ray

    Institute of Scientific and Technical Information of China (English)

    陈海游; 张忠和; 秦志虹; 张海涛

    2014-01-01

    利用60Co-γ射线对胭脂虫孕卵期进行辐射试验,探讨60Co对胭脂虫生物学特征的影响.结果表明,高剂量(3000rad以上)辐射胭脂虫造成其子代性比严重失调,雄虫几乎为0,无法进行有性生殖,导致无法产生后代,高剂量辐射对雄虫的损伤极大.低剂量辐射(特别是500rad)下F1较对照:各龄级虫口数提高;成虫期雌虫数明显高于对照,雄虫数略高;孵化量、孵化率提高;单株雌成虫鲜重、干重均明显增重;怀卵量大幅增多;雌成虫个体鲜干重、鲜干比、体积无差异.对于500rad辐射的胭脂虫,F2在有效生殖率、鲜干重和鲜干比方面效果较F1还好,存在优势显效滞后现象.但连续培育3代后,与对照已无差异.

  10. Effects of 60Co gamma radiation on toxicity and hemorrhagic, myonecrotic, and edema-forming activities of Cerastes cerastes venom

    International Nuclear Information System (INIS)

    Antisera are used as effective antidotes against the local effects of snake bites. To improve antisera production and extend the life of surrogates used to produce antibodies, the chronic effects of venom toxicity must be reduced. The present study evaluated the effectiveness of gamma irradiation to reduce the local effects associated with viperid snake bites by evaluating in NMRI mice the toxicity and edematic, hemorrhagic, and myonecrotic activities of native and irradiated Cerastes cerastes venoms. These results indicated that the toxicity of irradiated venoms (1 and 2 kGy) decreased as compared with that of native venom. The edematic and hemorrhagic activities were also reduced in the detoxified samples, particularly with the 2-kGy radiation dose. Furthermore, the creatine phosphokinase (CPK) activity was significantly increased in the serum and decreased in the myocardium after envenomation with native venom, but no significant enzymatic changes were observed in mice envenomated with irradiated venom. Histopathologic evaluation showed that native venom caused severe degenerative changes in the myocardium. In the case of 2-kGy-irradiated venom, no tissue alterations were observed. These results indicate that irradiation of venom with a 2-kGy dose may offer an effective method for reducing the chronic toxic effects of venom in immunized animals. (author)

  11. Use of an external source (60Co) for 32P detection efficiency determination by the Cerenkov effect, in soil extracts

    International Nuclear Information System (INIS)

    The detection of 32P in aqueous extracts is usually made with the aid of a Geiger-Muller detector, with thin window and sample on a planchet. Presently the technique is being developed of detection of high energy beta particles emitters (32P, 42K, 86Rb) through the Cerenkov effect, using a commercial liquid scintillation system. This technique, despite being approximately 30 times more sensitive, has the inconvenience of varying the detection efficiency, mainly for color samples (soil extracts, for instance). From this stems the need for determining the detection efficiency for each sample. The internal standardization and channels ratio methods show a series of drawbacks, mainly the non-reutilization of the samples (1st method) and statistical uncertainty for low activity samples (2nd method). The elimination of these dreawbacks can be achieved through the utilization of the external standardization method. A 60Co source with 1,4 μCi activity has been adapted to the sample elevator of the detector system, and a comparison was made with the channels ratio method to evaluate the efficiency of 32P detection in soil extracts (P extraction and fractionation). The external standardization method showed to be more accurate, besides being influenced to a lesser degree by high voltage variation, sample volume and vial types. In the case of large samples, it is advisable to carry out detection in vials filled up to their full capacity; in the case of small samples, the whole volume should be transferred to the vials and completed up to 9 ml for nylon vials,10 ml for glass vials and 11 to 14 ml for polyethilene vials. On the other hand, plastic vials showed higher detection efficiency than ones. As to background radiation, the lowest rates were given by nylon vials and the highest by Beckman glass vials

  12. An integrated analysis of the life-shortening effects in mice of fission neutrons and /sup 60/Co γ rays

    International Nuclear Information System (INIS)

    Ten studies on the life-shortening and tumorigenic effects of /sup 60/Co γ rays and fission neutrons from the JANUS reactor have been completed. Young adult B6CF/sub 1/ males and females were exposed to brief single, 24 once-weekly, or 60 once-weekly doses of either radiation and followed for their lifetimes. Two studies involved γ rays delivered in five 22-h days per week for either 23 or 59 elapsed weeks. The mean aftersurvival (MAS) data were analyzed to determine the influence of total dose, sex, exposure pattern, radiation quality, and nonadditive interactions among these factors on dose-response relations between MAS and total dose. Each γ-ray-exposure pattern produces a unique, linear, dose-rate-dependent life-shortening coefficient, which ranges from 0.43 days lost/cGy for single doses to 0.08 days lost/cGy for single doses to 0.08 days lost/cGy for the continuous (59 five-day week) exposures. For total neutron doses below 15 cGy, there is no significant nonlinear (D/sup 2/) term and no apparent dose-rate effect. A significant negative D/sup 2/ term emerges at doses ≥ 20 cGy. Linear coefficients from progressive analyses of data between 1 and 40 cGy are between five and seven days lost/cGy for females and three and four days lost for males. Data between doses of 1 and ≥ 60 cGy are not depicted by a simple dose-response model. RBE/sub m/ lies between 40 and 60 for each sex and could reach 90, if sex differences are ignored

  13. Total dose effect on floating gate ROMs irradiated by Co-60 γ-rays or pulsed γ-rays

    International Nuclear Information System (INIS)

    Memory radiation effect studies in China have been mainly focused on functionality measurement. According to the present situation, threshold voltage testing method was presented on Floating Gate (FG) memory. FG EPROMs of different integration scales were pre-irradiation by UV lights, followed by irradiation of 60Co γ-rays, or pulsed γ-rays. The total dose effect similarities and differences were studied. The damage mechanism was analyzed. The results showed that unlike MOS and CMOS devices, FG memory threshold voltage shifted more due to pulsed irradiation than 60Co γ-ray irradiation. This is of important reference for choosing the device type for a certain system. (authors)

  14. New facility for post irradiation examination of neutron irradiated beryllium

    Energy Technology Data Exchange (ETDEWEB)

    Ishitsuka, Etsuo; Kawamura, Hiroshi [Oarai Research Establishment, Ibaraki-Ken (Japan)

    1995-09-01

    Beryllium is expected as a neutron multiplier and plasma facing materials in the fusion reactor, and the neutron irradiation data on properties of beryllium up to 800{degrees}C need for the engineering design. The acquisition of data on the tritium behavior, swelling, thermal and mechanical properties are first priority in ITER design. Facility for the post irradiation examination of neutron irradiated beryllium was constructed in the hot laboratory of Japan Materials Testing Reactor to get the engineering design data mentioned above. This facility consist of the four glove boxes, dry air supplier, tritium monitoring and removal system, storage box of neutron irradiated samples. Beryllium handling are restricted by the amount of tritium;7.4 GBq/day and {sup 60}Co;7.4 MBq/day.

  15. Irradiation effects on hydrases for biomedical applications

    Energy Technology Data Exchange (ETDEWEB)

    Furuta, Masakazu E-mail: mfuruta@riast.osakafu-u.ac.jp; Ohashi, Isao; Oka, Masahito; Hayashi, Toshio

    2000-03-01

    To apply an irradiation technique to sterilize 'Hybrid' biomedical materials including enzymes, we selected papain, a well-characterized plant endopeptidase as a model to examine durability of enzyme activity under the practical irradiation condition in which limited data were available for irradiation inactivation of enzymes. Dry powder and frozen aqueous solution of papain showed significant durability against {sup 60}Co-gamma irradiation suggesting that, the commercial irradiation sterilizing method is applicable without modification. Although irradiation of unfrozen aqueous papain solution showed an unusual change of the enzymatic activity with the increasing doses, and was totally inactivated at 15 kGy, we managed to keep the residual activity more than 50% of initial activity after 30-kGy irradiation, taking such optimum conditions as increasing enzyme concentration from 10 to 100 mg/ml and purging with N{sub 2} gas to suppress the formation of free radicals. (author)

  16. Inactivation effect of 60Co gamma ray on cysticerci of Taenia solium in pork%60Coγ射线对猪肉中囊尾蚴的辐照杀灭效应

    Institute of Scientific and Technical Information of China (English)

    钟凯; 高翔; 计融

    2011-01-01

    Objective revealing the relationship between irradiation dose and inactivation ratio for eysticerei of Taenia solium in pork, determining the minimal dose for totally killing cysticerci and providing scientific evidence for setting up relevant hygienic standards of radiation. Methods After Taenia solium infected pork being treated with different irradiation doses; cysticerci were separated and incubated in fresh swine bile. The adjusted inactivation ratio was calculated according to the survival rate. Results There was a linear correlation between irradiation dose and adjusted inactivation ratio, the D10 value was about 3.8 kGy, and a totally killing could be achieved by 4.5 kGy irradiation. Conclusion Irradiation at a dose of 4.5 kGy could be applied as the maximum dose to inactivate cysticerci of Taenia solium in pork processing.%目的 揭示猪肉中囊尾蚴灭活率与辐照剂量的相关关系及最小杀灭剂量,为制定相应辐照卫生规范提供科学依据.方法 以不同剂量Co γ射线处理感染囊尾蚴的猪肉,并用新鲜猪胆汁孵化剥离出的囊尾蚴,根据成活率计算校正灭活率.结果 辐照剂量与校正灭活率具有线性相关,D值约为3.8 kGy,4.5 kGy可完全杀灭囊尾蚴.结论 4.5 kGy γ射线对猪肉中囊尾蚴有很好的杀灭效果.

  17. Berberine Inhibited Radioresistant Effects and Enhanced Anti-Tumor Effects in the Irradiated-Human Prostate Cancer Cells

    OpenAIRE

    Hur, Jung-Mu; Kim, Dongho

    2010-01-01

    The purpose of this study was to elucidate the mechanism underlying enhanced radiosensitivity to 60Co γ-irradiation in human prostate PC-3 cells pretreated with berberine. The cytotoxic effect of the combination of berberine and irradiation was superior to that of berberine or irradiation alone. Cell death and Apoptosis increased significantly with the combination of berberine and irradiation. Additionally, ROS generation was elevated by berberine with or without irradiation. The antioxidant ...

  18. Influence of gamma ray irradiation on metakaolin based sodium geopolymer

    Energy Technology Data Exchange (ETDEWEB)

    Lambertin, D., E-mail: david.lambertin@cea.fr [CEA, DEN, DTCD/SPDE/LP2C, F-30207 Bagnols-sur-Cèze (France); Boher, C. [CEA, DEN, DTCD/SPDE/LP2C, F-30207 Bagnols-sur-Cèze (France); Dannoux-Papin, A. [CEA, DEN, DTCD/SPDE/LCFI, F-30207 Bagnols-sur-Cèze (France); Galliez, K.; Rooses, A.; Frizon, F. [CEA, DEN, DTCD/SPDE/LP2C, F-30207 Bagnols-sur-Cèze (France)

    2013-11-15

    Effects of gamma irradiation on metakaolin based Na-geopolymer have been investigated by external irradiation. The experiments were carried out in a gamma irradiator with {sup 60}Co sources up to 1000 kGy. Various Na-geopolymer with three H{sub 2}O/Na{sub 2}O ratios have been studied in terms of hydrogen radiolytic yield. The results show that hydrogen production increases linearly with water content. Gamma irradiation effects on Na-geopolymer microstructure have been investigated with porosity measurements and X-ray pair distribution function analysis. A change of pore size distribution and a structural relaxation have been found after gamma ray irradiation.

  19. Influence of gamma ray irradiation on metakaolin based sodium geopolymer

    Science.gov (United States)

    Lambertin, D.; Boher, C.; Dannoux-Papin, A.; Galliez, K.; Rooses, A.; Frizon, F.

    2013-11-01

    Effects of gamma irradiation on metakaolin based Na-geopolymer have been investigated by external irradiation. The experiments were carried out in a gamma irradiator with 60Co sources up to 1000 kGy. Various Na-geopolymer with three H2O/Na2O ratios have been studied in terms of hydrogen radiolytic yield. The results show that hydrogen production increases linearly with water content. Gamma irradiation effects on Na-geopolymer microstructure have been investigated with porosity measurements and X-ray pair distribution function analysis. A change of pore size distribution and a structural relaxation have been found after gamma ray irradiation.

  20. Alterations in immune responses in prenatally irradiated dogs

    International Nuclear Information System (INIS)

    Immunologic responses were studied in beagle dogs following prenatal (35 days gestation) irradiation to evaluate the effects of ionizing radiation on the developing immune system. Each dog received 1.5 Gy 60Co gamma irradiation or sham irradiation. Prenatally irradiated dogs exhibited a significant reduction in primary humoral antibody responses to inoculated sheep red blood cells, a T-dependent antigen, and a concurrent decrease in T-helper lymphocyte subpopulations in the peripheral blood at 3 to 4 months of age. Similarly, irradiated fetuses have been shown to have defects in epitheliostromal development of the thymus. It is suggested that the postnatal immunologic deficits may relate to the prenatal thymic injury

  1. The influence of organic compound addition on the mobility and biological availability of the {sup 137} Cs and {sup 60} Co on tropical soils; A influencia da adicao de composto organico na mobilidade e biodisponibilidade do {sup 137} Cs e do {sup 60} Co em solos tropicais

    Energy Technology Data Exchange (ETDEWEB)

    Portilho, Antonio Passos

    2005-07-01

    The hole of manure in the mobility and bioavailability of {sup 137}Cs and {sup 60}Co in Brazilian soil was investigated. For this study an Oxisol was selected, for being the soil of larger distribution in the country, characterized by a low content in nutrients and organic substances; a Nitisol, was also selected as representative of soils with average fertility and an Organo sol, as representative of soils with high content of organic matter. These soils were transferred to an experimental area in the Instituto de Radioprotecao e Dosimetria, conditioned in plastic pots and spiked with {sup 137}Cs e {sup 60}Co. To follow the root uptake of {sup 137}Cs and {sup 60}Co we cultivated radish (Raphanus sativus, L.), in the pots receiving different doses of manure: a dose recommended for radish (2 kg/m{sup 2}); a half of the recommended dose; the double of the recommended dose and without manure addition. Only the Organo sol did not received manure. Moreover, it was investigated the potential mobility of {sup 137}Cs and {sup 60}Co associated to the unstable compounds under natural soil environment, such as the organic substance, oxides of iron etc, through the method of sequential chemical extraction. The main results show that soil to plant transfer factor (TF) for {sup 137}Cs decreases after the addition of 4 kg/m{sup 2} of manure in the Nitisol (from 0.44 to 0.06) and in the Oxisol (from 0,44 to 0,11). In relation to {sup 60}Co the TF reduced from 14,73 for 0,15 in Nitisol and from 11,25 for 0,15 to Oxisol. In the Organosols the TF values were low and comparable with values obtained in soil that received at least a dose of manure: 0,11 for {sup 137}Cs and 2,64 for {sup 60}Co. It was also observed, that the TF for {sup 137}Cs diminish with the increase of the nutrient contents (K, Ca and Mg) when manure is applied. It was verified, through the results of sequential extraction, that {sup 137}Cs was mainly associated to iron oxides in all types of studied soils. In the

  2. Soil compaction and gamma radiation ({sup 60}Co) in the development of the cowpea beans [Vigna unguiculata, (L) Walp]; Compactacao do solo e radiacao gama ({sup 60}Co) no desenvolvimento do feijao caupi [Vigna unguiculata, (L) Walp

    Energy Technology Data Exchange (ETDEWEB)

    Viana, Eliane Ferreira; Colaco, Waldeciro [Universidade Federal de Pernambuco (UFPE), Recife, PE (Brazil). Dept. de Energia Nuclear. Radioagronomia]. E-mails: aroucha@ufpe.br; wcolaco@ufpe.br

    2005-08-15

    The objective is to investigate the effect of compaction and increased doses of gamma radiation on the development of cowpea [Vigna unguiculata, (L) Walp] var. IPA-206 cultivated in a Neossolo fluvic soil, artificially compacted. Significant differences were observed in plant height, with the increase doses in soil density (1.30 Mg.m{sup -3} - compacted soil and 1.70 Mg.m{sup -3} - non-compacted soil), and in response to increased doses of irradiation (y-rays) [ [0, 100, 200 and 300 Gy). The diameter of the stem was significantly reduced in response to the increase in soil density, however the same did not occur in relation to the doses of irradiation (y-rays) holding capacity of the soil was reduced in response to the increase in soil density. (author)

  3. APMP key comparison for the measurement of air kerma for 60Co (APMP.RI(I)-K1.1)

    Science.gov (United States)

    Webb, D. V.; Lee, J.-H.; Budiantari, C. T.; Laban, J.; Saito, N.; Srimanoroth, S.; Khaled, N. E.

    2016-01-01

    The results are reported for an APMP.R(I)-K1.1 comparison that extends the regional comparison of standards for air kerma APMP.R(I)-K1 to several laboratories unable to participate earlier. The comparison was conducted with the goal of supporting the relevant calibration and measurement capabilities (CMCs) planned for publication by the participant laboratories. The comparison was conducted by the pilot laboratory, the Australian Radiation Protection and Nuclear Safety (ARPANSA), Australia, supported by the Institute of Nuclear Energy Research (INER), Taiwan, in a modified ring-shaped arrangement from September 2009 to November 2010, in parallel with an APMP.R(I)-K4 comparison being piloted by the INER. The laboratories that took part in the comparison were the ARPANSA, the Centre of Technology of Radiation Safety and Metrology (PTKMR-BATAN), Indonesia, the Division of Radiation and Medical Devices (DMSC), Thailand, the INER, the National Centre for Radiation Science (NCRS), New Zealand, the National Institute for Standards (NIS), Egypt and the National Metrology Institute of Japan (NMIJ/AIST), Japan. The two primary laboratories, ARPANSA and NMIJ, were chosen as the linking laboratories. Three ionization chambers were used as transfer instruments to be calibrated in terms of air kerma in 60Co radiotherapy beams. The comparison result is based on the ratio between the air kerma calibration coefficients (NK) determined by the participants and the mean of the results of the linking laboratories. The mean comparison ratio was found to be within 0.5 % of the key comparison reference value KCRV. The largest deviation between any two comparison ratios for the three chambers in terms of air kerma was 2.0 %. An analysis of the participant uncertainty budgets enabled the calculation of degrees of equivalence (DoE) in terms of the deviations of the results and their associated uncertainties. As a result of this APMP comparison, the BIPM key comparison database (KCDB) should

  4. Afterloading intracavitary irradiation and expanding stent for malignant biliary obstruction

    International Nuclear Information System (INIS)

    A double lumen catheter was developed as an apllicator for the remote afterloading (RALS) of 60Co source for the intracavitary irradiation of an obstructed common bile duct caused by carcinoma of the gallbladder. This was followed by the placement of nylon-covered expandable metallic stents to maintain patency. This combination effectively provided palliation. (author)

  5. The Effect of 5'-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co γ-ray

    Directory of Open Access Journals (Sweden)

    Cuilin Cheng

    2013-12-01

    Full Text Available Understanding the protection mechanism of 5'-AMP requires comprehensive knowledge of the proteins expressed during the period that the body is exposed to irradiation. Proteomics provides the tools for such analyses. Here, the experimental ICR mice were divided into three groups (normal group, model group and 5'-AMP + irradiation group. After different treatment, the hepatic total protein of each animal in three groups was separated by two-dimensional gel electrophoresis (2-DE. 2-DE analysis revealed fifty-eight protein spots were differentially expressed in comparison to three groups. From 58 protein spots, we selected nine spots to identify by MALDI-TOF-MS and received credible results. They were determined to be type I arginase, annexin A5, regucalcin, catalase, Tpm3 protein, Pdia4 protein, 14-3-3 protein epsilon, NAD-Malate dehydrogenase and heat shock protein 90. Considering the characteristic of these proteins, we proposed a possible protection pathway.

  6. (60)Co in cast steel matrix: A European interlaboratory comparison for the characterisation of new activity standards for calibration of gamma-ray spectrometers in metallurgy.

    Science.gov (United States)

    Tzika, Faidra; Burda, Oleksiy; Hult, Mikael; Arnold, Dirk; Marroyo, Belén Caro; Dryák, Pavel; Fazio, Aldo; Ferreux, Laurent; García-Toraño, Eduardo; Javornik, Andrej; Klemola, Seppo; Luca, Aurelian; Moser, Hannah; Nečemer, Marijan; Peyrés, Virginia; Reis, Mario; Silva, Lidia; Šolc, Jaroslav; Svec, Anton; Tyminski, Zbigniew; Vodenik, Branko; Wätjen, Uwe

    2016-08-01

    Two series of activity standards of (60)Co in cast steel matrix, developed for the calibration of gamma-ray spectrometry systems in the metallurgical sector, were characterised using a European interlaboratory comparison among twelve National Metrology Institutes and one international organisation. The first standard, consisting of 14 disc shaped samples, was cast from steel contaminated during production ("originally"), and the second, consisting of 15 similar discs, from artificially-contaminated ("spiked") steel. The reference activity concentrations of (60)Co in the cast steel standards were (1.077±0.019) Bqg(-1) on 1 January 2013 12h00 UT and (1.483±0.022) Bqg(-1) on 1 June 2013 12h00 UT, respectively.

  7. (60)Co in cast steel matrix: A European interlaboratory comparison for the characterisation of new activity standards for calibration of gamma-ray spectrometers in metallurgy.

    Science.gov (United States)

    Tzika, Faidra; Burda, Oleksiy; Hult, Mikael; Arnold, Dirk; Marroyo, Belén Caro; Dryák, Pavel; Fazio, Aldo; Ferreux, Laurent; García-Toraño, Eduardo; Javornik, Andrej; Klemola, Seppo; Luca, Aurelian; Moser, Hannah; Nečemer, Marijan; Peyrés, Virginia; Reis, Mario; Silva, Lidia; Šolc, Jaroslav; Svec, Anton; Tyminski, Zbigniew; Vodenik, Branko; Wätjen, Uwe

    2016-08-01

    Two series of activity standards of (60)Co in cast steel matrix, developed for the calibration of gamma-ray spectrometry systems in the metallurgical sector, were characterised using a European interlaboratory comparison among twelve National Metrology Institutes and one international organisation. The first standard, consisting of 14 disc shaped samples, was cast from steel contaminated during production ("originally"), and the second, consisting of 15 similar discs, from artificially-contaminated ("spiked") steel. The reference activity concentrations of (60)Co in the cast steel standards were (1.077±0.019) Bqg(-1) on 1 January 2013 12h00 UT and (1.483±0.022) Bqg(-1) on 1 June 2013 12h00 UT, respectively. PMID:27236833

  8. Experimental studies on prevention of trichinosis by food irradiation (Co60)

    International Nuclear Information System (INIS)

    A systemic study was performed to elucidate the effects of 60Co irradiation on maturation or fecundity of Trichinella spiralis by assessing from parasitological, morphological and immunological points of view. Pieces of muscle tissue of mice infected with T. spiralis were irradiated with 60Co at doses of 50, 75, 100, 150 and 200 Gy. These irradiated tissue (Experimental group) and non-irradiated tissues (Control group) were fed to healthy mice. Half of the mice were sacrificed 6 days after the ingestion. The number of adult worms were counted and subjected to statistical analysis, which disclosed a good correlation between the dose of 60Co irradiation and worm damage; the higher the dose the more damage parasites had, and no adults were recovered if the tissue had been irradiated with more than 150 Gy prior to infection. The same tendency was true for the histopathology of the intenstines of host mice; the higher the dose the less tissue damage. Circulating antigens of T. spiralis and antibodies against T. spiralis were detected in every serum examined by ELISA. The remaining mice were sacrificed 30 days after the ingestion. The numbers of muscle larvae were counted and subjected to statistical analysis, which disclosed a good correlation between th dose of 60Co irradiation and worm damage, and no larvae were recovered if the tissue had been irradiated with more than 75Gy. Higher concentrations of circulating antigens of T. spiralis and antibodies against T. spiralis were detected in the serum samples taken with muscle larvae. Thus this study established that 60Co irradiation of more than 150 Gy caused complete damage on maturation, and that irradiation of more than 75 Gy caused complete damage on the fecundity of T. spimalis. (author)

  9. Thermal analysis applied to irradiated propolis

    Energy Technology Data Exchange (ETDEWEB)

    Matsuda, Andrea Harumi; Machado, Luci Brocardo; Mastro, N.L. del E-mail: nelida@usp.br

    2002-03-01

    Propolis is a resinous hive product, collected by bees. Raw propolis requires a decontamination procedure and irradiation appears as a promising technique for this purpose. The valuable properties of propolis for food and pharmaceutical industries have led to increasing interest in its technological behavior. Thermal analysis is a chemical analysis that gives information about changes on heating of great importance for technological applications. Ground propolis samples were {sup 60}Co gamma irradiated with 0 and 10 kGy. Thermogravimetry curves shown a similar multi-stage decomposition pattern for both irradiated and unirradiated samples up to 600 deg. C. Similarly, through differential scanning calorimetry , a coincidence of melting point of irradiated and unirradiated samples was found. The results suggest that the irradiation process do not interfere on the thermal properties of propolis when irradiated up to 10 kGy.

  10. Effect of cobalt 60 gamma-ray irradiation on the hatching process of chicken eggs

    International Nuclear Information System (INIS)

    An experiment on fertilized chicken eggs was carried out to determine the effects of 60Co irradiation on the embryos, their fatality, and growth impairment or deformity, in particular. The experimental groups, consisting of 10 eggs each, recieved a 60Co irradiation of 50 to 2,000 rads on any one day between day 0 and day 20 of incubation. The larger the irradiation dose, the greater was the number of dead embryos. The fatality was higher in the groups receiving irradiation in the earlier stage (1st week). The resultant death was a chronic one. The irradiation also caused body weight decrease and growth impairment. A decrease in the brain and liver weights was noted, suggesting insufficiency in these organs. Deformity occurred in 4%, most of which involved impairments of skeletal growth, of the bones of the extremities and the bill, in particular. Administration of the SH amino acid, cysteine tended to alleviate the adverse effects of the 60Co irradiation. These results for fertilized chicken eggs suggest the possibility of abortion and the occurrence of deformities in human fetuses if they should be subjected to 60Co irradiation. (author)

  11. A water calorimeter for on-site absorbed dose to water calibrations in (60)Co and MV-photon beams including MRI incorporated treatment equipment.

    Science.gov (United States)

    de Prez, Leon; de Pooter, Jacco; Jansen, Bartel; Aalbers, Tony

    2016-07-01

    In reference dosimetry the aim is to establish the absorbed dose to water, D w, under reference conditions. However, existing dosimetry protocols are not always applicable for rapidly emerging new treatment modalities. For primary standard dosimetry laboratories it is generally not feasible to acquire such modalities. Therefore it is strongly desired that D w measurements with primary standards can be performed on-site in clinical beams for the new treatment modalities in order to characterize and calibrate detectors. To serve this need, VSL has developed a new transportable water calorimeter serving as a primary D w standard for (60)Co and MV-photons including MRI incorporated treatment equipment. Special attention was paid to its operation in different beam geometries and beam modalities including the application in magnetic fields. The new calorimeter was validated in the VSL (60)Co beam and on-site in clinical MV-photon beams. Excellent agreement of 0.1% was achieved with previous (60)Co field calibrations, i.e. well within the uncertainty of the previous calorimeter, and with measurements performed in horizontal and vertical MV-photon beams. k Q factors, determined for two PTW 30013 ionization chambers, agreed very well with available literature data. The relative combined standard uncertainty (k  =  1) for D w measurements in (60)Co and MV-photons is 0.37%. Calibrations are carried out with a standard uncertainty of 0.42% and k Q -factors are determined with a relative standard uncertainty of 0.40%. PMID:27300589

  12. A water calorimeter for on-site absorbed dose to water calibrations in 60Co and MV-photon beams including MRI incorporated treatment equipment

    Science.gov (United States)

    de Prez, Leon; de Pooter, Jacco; Jansen, Bartel; Aalbers, Tony

    2016-07-01

    In reference dosimetry the aim is to establish the absorbed dose to water, D w, under reference conditions. However, existing dosimetry protocols are not always applicable for rapidly emerging new treatment modalities. For primary standard dosimetry laboratories it is generally not feasible to acquire such modalities. Therefore it is strongly desired that D w measurements with primary standards can be performed on-site in clinical beams for the new treatment modalities in order to characterize and calibrate detectors. To serve this need, VSL has developed a new transportable water calorimeter serving as a primary D w standard for 60Co and MV-photons including MRI incorporated treatment equipment. Special attention was paid to its operation in different beam geometries and beam modalities including the application in magnetic fields. The new calorimeter was validated in the VSL 60Co beam and on-site in clinical MV-photon beams. Excellent agreement of 0.1% was achieved with previous 60Co field calibrations, i.e. well within the uncertainty of the previous calorimeter, and with measurements performed in horizontal and vertical MV-photon beams. k Q factors, determined for two PTW 30013 ionization chambers, agreed very well with available literature data. The relative combined standard uncertainty (k  =  1) for D w measurements in 60Co and MV-photons is 0.37%. Calibrations are carried out with a standard uncertainty of 0.42% and k Q -factors are determined with a relative standard uncertainty of 0.40%.

  13. Effects of Nitric Oxide on Growth and Sensitivity of Human Glioma A172 Cells to 60Co and X-ray Radiation

    Institute of Scientific and Technical Information of China (English)

    QiuRong; LiuBing; GuoChuanling; LiWenjian; MaQiufeng; LiSha

    2003-01-01

    Nitric oxide (NO)has been implicated both in regression and progression of tumors due to its production by both tumor cells and infiltrating lymphocytes. Ionizing radiation causes the regression of tumors, and can augment the production of NO by macrophages in vitro. The authors examined the effect of nitric oxide (NO) generating agent sodium nitroprusside (SNP) on the growth and radiosensitivity of cultured glioma A172 cells to 60Co or X-ray radiation.

  14. Food irradiation in Malaysia

    International Nuclear Information System (INIS)

    Food irradiation has recently been visited as a technology that can contribute to the solution of problems associated with food preservation of Malaysia's agriculture produce and products thereby improving the economic status of the rural sector. However, the history of food irradiation in Malaysia is very recent. Research carried out on food irradiation only began in 1974 as a result of the installation of a 60Co facility (initially 10,000 Ci) at the National University of Malaysia. Since its installation several studies have been carried out pertaining to the food irradiation. Presently its development has been slow. Research in this area has been confined to laboratory scale and purely academic. This limitation is due to a number of reasons, among others are: a) limited number of facilities; b) lack of expertise to conduct its research; c) other preservation methods can be improved with lower capital output. An important step towards its development was made when Malaysia actively participated in the RCA/IAEA food irradiation project, viz. the irradiation of pepper which was carried out at the National University of Malaysia in the 80's. As a result of this venture, research and development activities in food irradiation have been geared toward semi-plot scale with the view ot commercialization in the future. In 1982, a group of researchers was formed to conduct feasibility studies using irradiation techniques in trying to overcome several problems associated with our local paddy and rice. Another group is being organized by the National University of Malaysia to look into the problems associated with the preservation of frozen shrimps. (author)

  15. Areal distribution of 60Co, 137Cs, and 90Sr in streambed gravels of White Oak Creek Watershed, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    The concentrations of 90Sr, 60Co, and 137Cs in streambed gravels from contaminated drainages in White Oak Creek Watershed were determined. Methods to determine the relative contributions of various sources to the total discharge from the watershed were developed. Principal sources of 90Sr were: ORNL plant effluents (50%), leaching from solid waste disposal area (SWDA) 4 (30%), and leaching from SWDA 5 (10%). Minor sources included SWDA 3, the Molten Salt Reactor Facility, and intermediate-level liquid waste pit 1 with each representing 4% or less of the total basin discharge. The cooling water effluent from the High-Flux Isotope Reactor was the dominant source of 60Co contamination in the watershed. ORNL plant effluents accounted for almost all the 137Cs discharge from White Oak Creek basin. Downstream radionuclide concentrations were constant until significant dilution by other tributaries occurred. Any future activities giving rise to additional contamination can now be identified. Distribution coefficients between streambed gravels and streamwater for 85Sr, 60Co, and 137Cs were 50, 560, and 8460 ml/g, respectively. An abridged radiochemical fractionation developed for 90Sr was found to be as accurate and precise for these samples as the standard 90Sr method above levels of 2 dpm/g

  16. Comparisons of the standards for air kerma of the PTB and the BIPM for {sup 60}Co and {sup 137}Cs gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Allisy-Roberts, P.J.; Burns, D.T. [Bureau International des Poids et Mesures (BIPM), 92 - Sevres (France); Buermann, L.; Kramer, H.M. [Physikalisch-Technische Bundesanstalt, Braunschweig (Germany)

    2005-11-15

    Direct comparisons of the standards for air kerma of the Physikalisch-Technische Bundesanstalt (PTB, Germany) and of the Bureau International des Poids et Mesures (BIPM) were carried out in the {sup 60}Co and {sup 137} Cs radiation beams of the BIPM in 2000. The results, expressed as ratios of the PTB and the BIPM standards for air kerma, indicate a relative difference in {sup 60}Co of 9.9 x 10{sup -3} with a combined standard uncertainty of 1.8 x 10{sup -3}, and in {sup 137}Cs of 6.4 x 10{sup -3} with a combined standard uncertainty of 2.8 x 10{sup -3}. The earlier comparisons in {sup 60}Co {gamma} rays made in 1971 (direct) and 1989 (indirect) resulted in an agreement of the two standards within 2 x 10{sup -3}. The differences obtained now are due to the application of new correction factors for wall effects and point source non-uniformity of the beam, k{sub wall} and k{sub pn}, for the PTB standards, which were calculated using Monte Carlo methods. (authors)

  17. In vivo and in vitro experimental study on cervix cancer with combination of HSV-TK/GCV suicide gene therapy system and 60Co radiotherapy

    International Nuclear Information System (INIS)

    Objective: To evaluate the killing effect of HSV-TK/GCV suicide gene therapy system combined with 60Co radiotherapy on human cervical cancer HeLa cell line in vivo and in vitro, and to explore radiosensitization by the HSV-TK/GCV system. Methods: The HSV-TK/GCV suicide gene therapy system and 60Co radiotherapy were used separately or in combination for human cervical cancer HeLa cell line in vivo and in vitro to compare their effects. Colony formation test and the rate of radiosensitization effect(E/O) were employed to observed the radiosensitization by the HSV-TK/GCV system. Results: The HSV-TK/GCV suicide gene therapy system showed strong therapeutic effects on HeLa cells both in vitro and in vivo (the inhibition rates were 45.8% and 39.5%, respectively). Moreover, the combined application of gene therapy and radiotherapy exhibited stronger therapeutic effects in vitro and in vivo (the inhibition rate was 87.5% in vitro, and was 87.9% in vivo) (P 1.4), indicating the HSV-TK/GCV system could exert a sensitizing effect on 60Co radiotherapy of the transplanted human cervical cancer cells in nude mice. Conclusion: The HSV-TK/GCV system has radiosensitizationaction. Gene therapy combined with radiotherapy may be a good supplementary method for synthetic treatment of cervical cancer. (authors)

  18. Leaching behavior of {sup 60}Co and {sup 137}Cs from spent ion exchange resins in cement-bentonite clay matrix

    Energy Technology Data Exchange (ETDEWEB)

    Plecas, Ilija E-mail: iplecas@vin.bg.ac.yu; Pavlovic, Radojko; Pavlovic, Snezana

    2004-05-01

    The leach rate of {sup 60}Co and {sup 137}Cs from two different ion exchange resins: (a) spent cation exchange resins and (b) spent mix bead ion exchange resins in cement-bentonite matrix has been studied. The solidification matrix was a standard Portland cement mixed with 290-350 kg/m{sup 3} spent cation exchange resins, with or without 2-5% of bentonite clay. The leach rates from the cement-bentonite matrix as {sup 60}Co: (4.2-7.3) x 10{sup -5} cm/d, and for {sup 137}Cs: (3.2-6.6) x 10{sup -5} cm/d, after 245 days were measured. From the leaching data the apparent diffusivity of cobalt and cesium in cement-bentonite clay matrix with a waste load of 290-350 kg/m{sup 3} spent cation exchange resins was measured as {sup 60}Co: (1.0-4.0) x 10{sup -6} cm{sup 2}/d and for {sup 137}Cs: (0.5-2.6) x 10{sup -4} cm{sup 2}/d after 245 days. These results are part of a 20-year mortar and concrete testing project which will influence the design of radioactive waste management for a future Serbian radioactive waste disposal center.

  19. A biological effectiveness study on chromosomal aberrations induced by fission neutrons versus 60Co γ-rays in human peripheral blood lymphocytes

    International Nuclear Information System (INIS)

    Objective: Whole blood lymphocytes samples being exposed to neutrons of 18 MeV energy and 60Co γ-rays respectively, both good dose-response relationships and relative biological effectiveness (RBE) were derived. Methods: Heparinized whole blood samples were exposed to neutrons and 60Co γ-rays, respectively. Radiation doses were from 0.5 Gy to 3.0 Gy. Dose rate was 0.2 Gy/min. Unstable chromosomal aberrations dicentrics and centric rings (dic+r), the same as Micronuclei in binucleated cells, were scored. Relative biological effectiveness (RBE) values of dic+rand Micronucleus were derived. Results: Chromosomal aberrations (dic+r) and Micronucleus induced by either neutrons or 60Co γ-rays had a good dose-response relationship. RBE value of chromosomal aberrations, exposed neutrons at 0.5-3.0 Gy, ranged from 1.59 to 2.81, similarly, micronucleus from 1.23 to 2.14. Conclusion: linear-quadratic dose-response was found for the induction of dic+r and Micronucleus in human lymphocytes exposed in vitro to neutrons of 18 MeV energy. neutrons has higher biological effectiveness in low doses. (authors)

  20. On the efficacy and safety of vaccination with live tachyzoites of Neospora caninum for prevention of neospora-associated fetal loss in cattle.

    Science.gov (United States)

    Weber, Fred H; Jackson, James A; Sobecki, Brian; Choromanski, Les; Olsen, Mary; Meinert, Todd; Frank, Rodney; Reichel, Michael P; Ellis, John T

    2013-01-01

    Infection of cattle with Neospora caninum may result in abortion or the birth of a congenitally infected calf. Vaccination with live N. caninum protects against experimental infection of cattle and mice, and the naturally attenuated Nc-Nowra strain of N. caninum is of particular interest as a potential vaccine candidate. Vaccination of heifers prior to breeding with live Nc-Nowra tachyzoites by either the subcutaneous or the intravenous route reduced the rate of abortion and the presence of the parasite in calves as determined by PCR and serology after infection of cows with a virulent isolate. Protected fractions were 55.6% to 85.2% depending on the route of vaccination and growth conditions of the vaccine strain, with cryopreserved Nc-Nowra tachyzoites being less effective, with a 25.9% protected fraction. Vaccination appeared to reduce the rate of pregnancy after artificial insemination in some groups compared to nonvaccinated, nonchallenged controls. One animal that was vaccinated but not challenged experienced an abortion, but Nc-Nowra could not be detected in any of the cows in this group or their progeny. This study confirms that live vaccination can be an effective method of preventing neosporosis in cattle and yet highlights the technical hurdle of preservation of live parasites that must be overcome for a vaccine to be commercially successful.

  1. Genetic effects of a combination of chronic 60Co-γ-radiation and lead nitrate on barley waxy-locus

    International Nuclear Information System (INIS)

    Using a highly sensitive test-system of a waxy change in pollen grains of waxy barley it was shown that chronic irradiation at dose-rates of 4.2-10.8x10-5 Gy/c increased the level of the induced waxy-reversions by 3.6-8.0 times in comparison with the spontaneous level. The introduction of lead nitrate to a soil-sand mixture modified the value of mutagenic and recombinogenic effects of radiation on barley waxy-locus

  2. The use of ionising radiation from 60CO gamma source in controlling mouldiness in dried cocoa beans

    International Nuclear Information System (INIS)

    Mouldiness in stored cocoa beans in Ghana and the production of aflatoxin have been studied. Based on actual weight of discarded beans, mouldy beans have been estimated to constitute 0.13 % and 0.00002 % of marketable beans at the farmers' level and the buying agents' depots respectively in the Tafo District. This is contrasted with an estimated value of 0.16 % obtained in a questionnaire type study involving farmers. Estimated mouldy beans at the Tema port was 0.69 % per year (based on the cut test) representing a financial loss of $1,688,637.19 per year at $989/T should the mouldy beans be discarded. Fifty-eight (58) internally- and externally- borne fungal species were isolated from dried cocoa beans. Of these, forty-eight (48) were internally- borne and ten (10) were superficial. Twenty-nine (29) of the internally occurring fungi have been recorded for the first time on cocoa beans in Ghana. Twenty-six (26) of the fungi isolated belong to Aspergillus group. They included A. parasiticus and A. flavus, which can produce aflatoxins. Five (5) belong to Penicillium, eight (8) to Fusarium and nineteen (19) to other species. Ionising radiation effectively controlled fungi associated with mouldiness in cocoa beans in a dose - dependent manner. A radiation dose of 6 kGy completely inactivated the moulds. A. flavus and A. tamarii were the most radiation - resistant moulds encountered. The moisture content of the beans before, during and after irradiation influenced the effect of radiation. The relative humidity during storage and the type of packaging also influenced the radiation effect. Conidia of A. flavus subjected to moist heat at temperatures 20 0C to 60 0C for 2.5, 5 and 10 min respectively were not significantly affected by heating up to 50 0C. Heating an aqueous conidial suspension at 60 0C for at least 2.5 min reduced the number of fungal colonies by at least 5 log cycles when the suspension was assayed on agar plate media. Heating at 59 0C for 10 min

  3. Brain anomalies induced by gamma irradiation in prenatal period

    International Nuclear Information System (INIS)

    Gamma irradiation has been utilized in order to produce cortical and callosal abnormalities. We have also checked for the presence of the aberrant longitudinal bundle in the brains of mice born acallosal due to prenatal irradiation is also checked. Pregnant mice were exposed to gamma irradiation from a 60 Co source at 16, 17 and 19 days of gestational age (E 16, E 17 and E 19) with total doses of 2 Gy and 3 Gy. At 60 days postnatal the offspring of irradiated animals were intra cardiac perfused, the brains were removed from the cranio and cut into coronal or para sagittal sections. (author)

  4. Fungal evaluation on green tea irradiated with different water activities

    International Nuclear Information System (INIS)

    The aim of this study was evaluate the fungal contamination in green tea irradiated with different radiation doses and water activities. Samples were irradiated in 60Co irradiator at doses of 0, 2.5, 5.0, 7.5 and 10.0kGy with three different water activities. In the sample with decreased water activity, the count of fungi was lower than others samples followed by original Aw and the samples with the higher water activity, however there is no difference between the increased and decreased water activities samples after the irradiation on fungi contamination at dose of 2.5 kGy. (author)

  5. Effect of gamma-irradiation on disinfectant, 1

    International Nuclear Information System (INIS)

    The effect of 60Coirradiation on the antibacterial activity of chlorhexidine hydrochloride was examined and the following results were obtained. 1. The radioresistance of B. pumilus ATCC 27142 spore suspended in the solution of chlorhexidine hydrochloride was apparently higher than that suspended in distilled water. 2. The antibacterial activity of chlorhexidine hydrochloride irradiated in wet state decreased with an increase of irradiation dose, but that in dry state did not decrease. Upon irradiating chlorhexidine hydrochloride adsorbed on cellulose product, the antibacterial acticity showed a similar tendency. (auth.)

  6. A study of irradiation in the treatment of wastewater

    Science.gov (United States)

    Bao, Huaying; Liu, Yuanxia; Jia, Haishun

    2002-03-01

    A grafting copolymer of starch and acrylamide was prepared by 60Co- γ pre-irradiation. After purification, the copolymer was modified by a cationic reaction to form a cationic copolymer. The structure of the cationic copolymer was identified by IR and NMR spectroscopy. Using the industrial and sanitary municipal wastewater from the Factory of Wastewater Treatment of Gaobeidian in Beijing as the study sample, three-treatment methods: flocculation deposition, flocculation deposition combined with γ irradiation and the direct irradiation were carried out. COD was applied to evaluate the treatment effect. The preliminary results show that the method of flocculation deposition combined with γ irradiation was effective than the other two.

  7. Fungal evaluation on green tea irradiated with different water activities

    Energy Technology Data Exchange (ETDEWEB)

    Fanaro, Gustavo B.; Duarte, Renato C.; Rodrigues, Flavio T.; Villavicencio, Anna Lucia C.H., E-mail: gbfanaro@ipen.b, E-mail: villavic@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (CTR/IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Centro de Tecnologia das Radiacoes; Correa, Benedito, E-mail: correabe@usp.b [Universidade de Sao Paulo (USP), SP (Brazil). Inst. de Ciencias Biologicas. Dept. de Micologia

    2011-07-01

    The aim of this study was evaluate the fungal contamination in green tea irradiated with different radiation doses and water activities. Samples were irradiated in {sup 60}Co irradiator at doses of 0, 2.5, 5.0, 7.5 and 10.0kGy with three different water activities. In the sample with decreased water activity, the count of fungi was lower than others samples followed by original Aw and the samples with the higher water activity, however there is no difference between the increased and decreased water activities samples after the irradiation on fungi contamination at dose of 2.5 kGy. (author)

  8. Effect of gamma-irradiation on nucleic acids, proteins, respiration and phosphatase activity of carrot callus cultures

    International Nuclear Information System (INIS)

    Callus tissue cultures were subjected to 60Co qamma irradiation at 0.5 Krad and analysed for nucleic acids, proteins, respiration rate and phosphatase activity on 0, 10, 20 and 30 days. The RNA contents and respiratory rates were enhanced as a result of irradiation. The RNA contents were reduced than their non-irradiated counterparts. The acid phosphatase activity was enhanced immediately after irradiation, declined on 10th and 20th day and more thereafter. (author)

  9. Irradiation effects on plasma diagnostic components (2)

    International Nuclear Information System (INIS)

    Irradiation tests on a number of diagnostic components under fission neutrons, gamma-rays and 14 MeV neutrons have been carried out as a part of the ITER technology R and D program. UV range transmission losses of a KU-1 quartz were measured during 14 MeV neutron and 60Co gamma-ray irradiation. Significant transmission losses were observed in the wavelength of 200-300 nm. Five kinds of ITER round robin fibers were irradiated in JMTR and the 60Co gamma-ray irradiation facility. KS-4V, KU-H2G and F-doped fibers have a rather good radiation hardness, which might be available just outside of the vacuum vessel in ITER. Mica substrate bolometer was irradiated in JMTR up to 0.1 dpa. During the cool down phase of the first cycle all connections went open circuit. The use of gold meanders in the bolometer might be problematic in ITER. The magnetic probes were irradiated in JMTR. Drift of 10 - 40 mVs for 1000s was observed with a digital longterm integrator, however, which might be induced not only by RIEMF but also drift inside the integrator itself. ITER-relevant magnetic coil could be made with MI-cables, whose electric drift for 1000-s integration is less than 0.5 mVs. (author)

  10. 低剂量60Co-γ辐射对棉铃虫蛾羽化、寿命、趋光行为和性信息素滴度的影响%Effects of low-dose 60Co-γ radiation on the emergence, longevity,phototactic behavior and sex pheromone titer in Helicoverpa armigera (Lepidoptera: Noctuidae) adults

    Institute of Scientific and Technical Information of China (English)

    闫硕; 朱家林; 张璟; 朱威龙; 张青文; 刘小侠

    2012-01-01

    To illustrate the effects of low-dose 60Co-γ radiation on the development, phototactic behavior and sex pheromone titer in Helicoverpa armigera ( Hübner) , we examined the emergence rate, the deformity rate and longevity of H. armigera adults under five doses of radiation, and determined the rate of phototaxis and sex pheromone titer of H. armigera by phototactic behavior trial and pheromone extraction method. The results indicated that ( 1) there were no effects of 60Co-ü radiation on adult emergence, deformity and longevity of the moth except for the irradiation of 20 Gy, under which the emergence rate was remarkably decreased by 16. 67% ( female) and 20. 00% ( male) , and the deformity rate was increased by 10.00% (both female and male). (2) Both in photophase and scotophase, the rate of phototaxis and sex pheromone titer were increased. The biggest jump of the rate of phototaxis occurred in 3-day-old females in photophase (28. 33%±3. 33% -91. 67% ±4.41% ) , while that of sex pheromone titer occurred in 5-day-old females in scotophase (36. 27 ±4. 26 ng -59. 13 ±4. 63 ng) , indicating that this dose irradiation (50 Gy) probably promoted the occurrence of phototactic behavior and the production of sex pheromone, while the radiation at five doses had no significant effects on phototaxis. (3) There was a declining trend following an increasing trend of the rate of phototaxis and sex pheromone titer as age increased. The biggest jump of the rate of phototaxis occurred in females in photophase and males in scotophase both after radiation (91.67%±4.41% -3.33%±1.67%), while that of sex pheromone titer occurred in females in scotophase after radiation (71. 00 ± 5. 22 ng - 3. 63 ± 1.47 ng). (4) There were no obvious differences in the emergence rate, the deformity rate, longevity and the rate of phototaxis between females and males in most treatments. This research may provide some theoretical basis for exploring the changes in phototaxis and physiological and

  11. Dose in sensitive organs during the prostate treatment with a {sup 60}Co unit; Dosis en organos sensibles durante el tratamiento de prostata con una unidad de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Vega C, H. R. [Universidad Autonoma de Zacatecas, Unidad Academica de Estudios Nucleares, Apdo. Postal 336, 98000 Zacatecas (Mexico); Navarro B, J. A. [Universidad Autonoma de Zacatecas, Unidad Academica de Fisica, Carretera a la Bufa s/n, Zacatecas (Mexico); Perez A, M. L.; Perez L, L. H., E-mail: fermineutron@yahoo.com [Instituto Zacatecano del Tumor, A. C., Lago de la Encantada No. 294, Fracc. Lomas del Lago, Zacatecas (Mexico)

    2012-10-15

    The absorbed dose by the bladder, the rectum and the gland thyroid was measured during a treatment applied for prostate cancer by means of a Cobalt 60 unit. The dose was measured using thermoluminescent dosimeters of the type TLD 100, with the values of the absorbed the values of the effective dose were calculated and was determined the probability of the development of a secondary cancer. Because these measurements cannot be made -in vivo- a phantom or mannequin was built with water that represents the hip and part of the torso of the human body and to represent the neck was used polyethylene. The study was carried out in the Instituto Zacatecano del Tumor that has a -cobalt bomb- which is used to treat oncology patients, during the phantom irradiation a dose of 200 c Gy was applied of this dose the bladder received 96.7%, the rectum 100.8% and the gland thyroid 0.3%. The dose received by the rectum and the bladder is due to the therapeutic beam while the dose received by the thyroid is due to the dispersed radiation by the phantom. The probability that in these organs a new neoplasm is developed is of 0.033% for the bladder, 0.157% for the rectum and 7.8 x 10{sup -5}% for the thyroid case. (Author)

  12. Description of morphologic characters in avocado trees (Persea americana. Mill) var. Hass, submitted to gamma radiation of 60 Co

    International Nuclear Information System (INIS)

    It was carried out the characterization of 77 trees of avocado ''Hass'' subjected to gamma radiation of Co60, in doses of 0, 5, 10,15, 20 and 25 Gy based on previous studies of radiosensitivity with ends of improvement located in the experimental center ''La Labor'' belonging to the foundation Salvador Sanchez Colin. CICTAMEX. S C. in Temascaltepec, Mexico. It was carried out the morphological characterization of this variety according to the nomenclature settled down by the International Office of Genetic Resources (IBPGRI), registering 27 variables for the description of the tree, 33 for description of the fruit and 14 for the characterization of the seed. Regarding the architectural characterization 10 variables were evaluated. Using gamma radiation of Co60 in the doses of 15 and 20 Gy it was detected a reduction of 22% in the portage of the tree, likewise had reduction the diameter of the trunk and of the foliage. It was found that the reduction in the behavior of the tree induces to a bigger variation of the form of the same one, in the distribution of the branches in the form of the fruit and in the form of the seed you Also detects a remarkable increment in the productivity of some hoist irradiated regarding the architecture of the tree one observes that the angle of inclination of the branches increase when being increased the radiation dose and that the longitude of the annual section of growth decreased significantly in the doses of 20 and 25 Gy. One observes a significant Increment in the I number of buds prolepticos in those hoist irradiated in the I finish mbdulo of rhythmic growth. Io that indicates a change substantial in the I inhabit of growth With the purpose of determining if the changes in morphology and architecture of the tree corresponded with the modification at level meristematico, they were carried out observations to the court microscope of having knitted apical vegetative meristematico in samples of you hoist of all the treatments

  13. An experimental study on cervix cancer with combination of HSV-TK/GCV suicide gene therapy system and 60Co radiotherapy

    International Nuclear Information System (INIS)

    To evaluate the killing effect of HSV-TK/GCV suicide gene therapy system combined with 60Co radiotherapy on human cervical cancer Hela cell line in vitro and in vivo, and to explore the radiosensitization by HSV-TK/GCV system. HSV-TK/GCV suicide gene therapy system and 60Co radiotherapy were used separately or in combination on human cervical cancer Hela cell line in vitro and in vivo to compare their effects. Colony formation test and the rate of radiosensitization effect (E/O) were employed to observed the radiosensitization by HSV-TK/GCV system. HSV-TK/GCV suicide gene therapy system had strong therapeutic effects on Hela cells in vitro and in vivo (the inhibition rates were 45.8% and 39.5%, respectively), moreover, the combined administration of gene therapy and radiotherapy had stronger therapeutic effects in vitro and in vivo (the inhibition rate was 87.5% in vitro, and the inhibition rate was 87.9% in vivo) (P < 0.01). The inhibition rate by radiotherapy alone was 42.4% in vitro and 35.8% in vivo. The sensitivity of combined therapy to radiotherapy increased more than that of therapy alone, the ability of colony formation decreased (P < 0.01). The rate of radiosensitivity effect (E/O) was 3.17(> 1.4), indicating HSV-TK/GCV system could exert a sensitizing effect on 60Co radiotherapy of the transplanted human cervical cancer cell in nude mice. HSV-TK/GCV system had radiosensitization. Gene therapy combined with radiotherapy may be a good supplementary method for cervix cancer synthetic treatment

  14. Transport of 85Sr, 60Co, 65Zn and 109Cd in two forest soils as observed in undisturbed soil columns

    International Nuclear Information System (INIS)

    Undisturbed soil columns of 20 cm in diameter and about 40 cm length were taken in a spruce stand (podsolic parabrown earth) and in a pine stand (podsol). In the laboratory the transport of 85Sr, 60Co, 65Zn and 109Cd was investigated under unsaturated conditions by periodical irrigation of the columns with rainwater and observation of the resulting depth profiles with a scanner technique as a function of time for three years. From the activity distributions obtained, the residence half-times and mean rates of migration of the radionuclides in the various soil horizons were evaluated with a compartment model. - In the top layer of the soil from the spruce stand (consisting of organic material only) the mean migration rate of 85Sr, 65Zn and 109Cd was 2.0 ± 0.05 cm/y and thus rather similar to that of 60Co (1.7 ± 0.3 cm/y). In the corresponding layer from the pine stand the mean migration rate of 85Sr, 65Zn and 109Cd was only 0.87 ± 0.06 cm/y (60Co:0.5 ± 0.10 cm/y). Since the water flux in the columns from the pine stand was even higher than in those from the spruce stand, the radionuclides seem to be sorbed stronger by organic material derived from pine needles than from spruce needles (the pH was about 3 in both soils). In the underlaying mineral soil the migration rate of the radionuclides was considerably faster than in the organic layer (between 2.5 and 10 cm/y), but a statistical difference between the values observed in the two stands or between the radionuclides was not apparent. (orig.)

  15. INCASOY-35: PRIMERA VARIEDAD DE SOYA OBTENIDA EN CUBA A PARTIR DEL EMPLEO DE TÉCNICAS DE IRRADIACIÓN DE RAYOS GAMMA DE 60Co

    Directory of Open Access Journals (Sweden)

    R. Ortiz

    2005-01-01

    Full Text Available La variedad INCASoy-35 fue obtenida a partir de la irradiación de semillas de la variedad INCASoy-15 con dosis de 240 Gy de rayos gamma 60Co; esta variedad se adapta a siembras de primavera, verano e invierno y puede alcanzar rendimientos de 3 t.ha-1 de granos. Tolera las principales plagas y enfermedades y, en especial, resiste el ataque de nemátodos Meloidogyne incognita. En la época adversa de primavera, las semillas han mostrado tolerancia al deterioro. Tiene capacidad de garantizar altas producciones con bajos insumos, por lo que resulta factible para la agricultura cubana.

  16. Soil-to-plant transfer of 54Mn, 60Co, 85Sr and 137Cs deposited during the growing season of potato

    International Nuclear Information System (INIS)

    To measure the soil-to-plant transfer factors (TFa, m2 kg-1-fresh) of radionuclides deposited during the growing season of potato, a radioactive solution containing 54Mn, 60Co, 85Sr and 137Cs was applied to the soil surfaces in soil boxes 2 d before seeding and three different times during the plant growth. For the Pre-Seeding Application (PSA), radionuclides were mixed with the topsoil (loamy sand and 5.2 in pH). The plant parts investigated were leaves, stems, tuber skin and tuber flesh. The TFa values of 54Mn, 60Co, 85Sr and 137Cs from the PSA were in the ranges of 1.9 x 10-4∼1.5 x 10-2, 1.8 x 10-4∼7.5 x 10-4, 4.0 x 10-4∼1.6 x 10-2, 1.5 x 10-4∼3.9 x 10-4, respectively, for different plant parts. The TFa values from the growing-time applications were on the whole a few times lower than those from the PSA. For 54Mn, 85Sr and 137Cs, the TFa values from the early- or middle-growth-stage application were higher than those from the late-growth-stage application, whereas the opposite was true for 60Co. Leaves and tuber flesh had the highest and lowest TFa values, respectively, in most cases. The total uptake from soil by the four plant parts was in the range of 0.05∼3.16%. In the third year following the PSA, the TFa values of 54Mn, 60Co and 137Cs were 11∼25%, 21∼25% and 38∼67% of those in the first year, respectively, depending on the plant parts. The present results can be used for estimating the radiological impact of an acute radioactive deposition during the growing season of potato and for testing the validity of relevant food-chain models

  17. Toxoplasmosis: The spread of tachyzoites, cysts and oocysts%弓形虫病:速殖子、包囊和卵囊的传播

    Institute of Scientific and Technical Information of China (English)

    汪涛; 汤自豪

    2012-01-01

    Toxo plasma gondii is widely distributed opportunistic pathogenic within the world, which can infect all warm-blooded animals including human and cause zoonotic toxoplasmosis , seriously throating human health and livestock production . This article focuses on the biological characteristics and transmission feature of Toxop lasma gondii tachyzoite , cysts and oocysts.%目的 弓形虫是一种世界范围内广泛分布的机会致病原虫,能感染包括人在内的所有温血动物,能引起人兽共患弓形虫病,严重威胁着人类健康和畜牧业生产.本文重点阐述弓形虫速殖子、包囊和卵囊的生物学特性和传播特点.

  18. Optimization of foaming properties of sludge protein solution by 60Co γ-ray/H2O2 using response surface methodology

    Science.gov (United States)

    Xiang, Yulin; Xiang, Yuxiu; Wang, Lipeng; Zhang, Zhifang

    2016-10-01

    Response surface methodology and Box-Behnken experimental design were used to model and optimize the operational parameters of foaming properties of the sludge protein solution by 60Co γ-ray/H2O2 treatment. The four variables involved in this research were the protein solution concentration, H2O2, pH and dose. In the range studied, statistical analysis of the results showed that selected variables had a significant effect on protein foaming properties. The optimized conditions contained: protein solution concentration 26.50% (v/v), H2O2 concentration 0.30% (v/v), pH value 9.0, and dose 4.81 kGy. Under optimal conditions, the foamability and foam stability approached 23.3 cm and 21.3 cm, respectively. Regression analysis with R2 value of 0.9923 (foamability) and 0.9922 (foam stability) indicated a satisfactory correlation between the experimental data and predicted values (response). In addition, based on a feasibility analysis, the 60Co γ-ray/H2O2 method can improve odor and color of the protein foaming solution.

  19. In situ determination of the distribution of 110Agm, 58Co, 60Co and 54Mn between fresh water and suspended matter

    International Nuclear Information System (INIS)

    In order to obtain data on the equilibrium conditions and the exchange kinetics of radionuclides between water and suspended matter in natural conditions, an in situ experiment was carried out in the nuclear power plant of Golfech (France). In this plant, an experimental physical model of river systems, represented by a channel, was built. Before being discharged into the Garonne river, part of the radioactive effluents, previously mixed with water originating from the cooling system, are diverted to a channel where different phenomena generally occurring in a river can be simulated. The experiment presented in the paper was undertaken during a scheduled discharge of low activity wastes; the suspended matter was sampled at four points along the channel using sequential filtration. The results give information about: (1) The influence of the particle size on the process of radionuclide adsorption by suspended matter. The uptake of radioactivity is generally higher for the smallest particles (size 110Agm, size 58Co, 60Co and 54Mn). (2) The exchange kinetics between water and suspended matter. Generally, radioactive equilibrium is rapidly attained. Consequently, it is possible to calculate the global distribution coefficient (Kd) value of each radionuclide (110Agm, 58Co, 60Co and 54Mn) and to determine exactly the specific Kd value for each granulometric class (class 1 > 10 μm; 10 μm < class 2 < 2.5 μm; 2.5 μm < class 3 < 0.5 μm). (author). 11 refs, 3 figs, 1 tab

  20. Supplementary comparison CCRI(I)-S2 of standards for absorbed dose to water in 60Co gamma radiation at radiation processing dose levels

    DEFF Research Database (Denmark)

    Burns, D. T.; Allisy-Roberts, P. J.; Desrosiers, M. F.;

    2011-01-01

    Eight national standards for absorbed dose to water in 60Co gamma radiation at the dose levels used in radiation processing have been compared over the range from 1 kGy to 30 kGy using the alanine dosimeters of the NIST and the NPL as the transfer dosimeters. The comparison was organized by the B......Eight national standards for absorbed dose to water in 60Co gamma radiation at the dose levels used in radiation processing have been compared over the range from 1 kGy to 30 kGy using the alanine dosimeters of the NIST and the NPL as the transfer dosimeters. The comparison was organized...... by the Bureau International des Poids et Mesures, who also participated at the lowest dose level using their radiotherapy-level standard for the same quantity. The national standards are in general agreement within the standard uncertainties, which are in the range from 1 to 2 parts in 102. Evidence of a dose...

  1. Magnetocaloric effect and critical behavior in melt-extracted Gd{sub 60}Co{sub 15}Al{sub 25} microwires

    Energy Technology Data Exchange (ETDEWEB)

    Xing, Dawei; Jiang, Sida; Chen, Dongming; Liu, Yanfen; Sun, Jianfei [School of Materials Science and Engineering, Harbin Institute of Technology (China); Shen, Hongxian [School of Materials Science and Engineering, Harbin Institute of Technology (China); Institute of Materials and Department of Physics, University of South Florida, Tampa, FL (United States); Liu, Jingshun [School of Materials Science and Engineering, Inner Mongolia University of Technology, Hohhot (China); Phan, Manh-Huong [Institute of Materials and Department of Physics, University of South Florida, Tampa, FL (United States); Wang, Huan; Qin, Faxiang [Institute for Composites Science and Innovation (InCSI), College of Materials Science and Engineering, Zhejiang University, Hangzhou (China)

    2015-09-15

    High-quality Gd{sub 60}Co{sub 15}Al{sub 25} microwires with an average diameter of 40 μm were successfully fabricated by the melt-extraction method. The as-cast microwires undergo a second-order paramagnetic to ferromagnetic (PM-FM) transition at ∝100 K. Large values of the magnetic entropy change (-ΔS{sub M} ∝9.73 J kg{sup -1} K{sup -1}) and the refrigerant capacity (RC ∝732 J kg{sup -1}) are achieved for a field change of 5 T. A careful analysis of critical exponents near the PM-FM transition indicates the significant effects of structural disorder on the long-range ferromagnetic interaction and the magnetocaloric response of the microwires. The excellent magnetocaloric properties make the Gd{sub 60}Co{sub 15}Al{sub 25} microwires very promising for use in magnetic refrigerators operating in the liquid nitrogen temperature range. (copyright 2015 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  2. Calculation of direct effects of 60Co gamma rays on the different DNA structural levels: A simulation study using the Geant4-DNA toolkit

    International Nuclear Information System (INIS)

    In this study, simple single strand breaks (SSB) and double strand breaks (DSB) due to direct effects of the secondary electron spectrum of 60Co gamma rays on different organizational levels of a volume model of the B-DNA conformation have been calculated using the Geant4-DNA toolkit. Result of this study for the direct DSB yield shows a good agreement with other theoretical and experimental results obtained by both photons and their secondary electrons; however, in the case of SSB a noticeable difference can be observed. Moreover, regarding the almost constant yields of the direct strand breaks in the different structural levels of the DNA, calculated in this work, and compared with some theoretical studies, it can be deduced that the direct strand breaks yields depend mainly on the primary double helix structure of the DNA and the higher-order structures cannot have a noticeable effect on the direct DNA damage inductions by 60Co gamma rays. In contrast, a direct dependency between the direct SSB and DSB yields and the volume of the DNA structure has been found. Also, a further study on the histone proteins showed that they can play an important role in the trapping of low energy electrons without any significant effect on the direct DNA strand breaks inductions, at least in the range of energies used in the current study

  3. Calculation of direct effects of {sup 60}Co gamma rays on the different DNA structural levels: A simulation study using the Geant4-DNA toolkit

    Energy Technology Data Exchange (ETDEWEB)

    Tajik, Marjan; Rozatian, Amir S.H. [Department of Physics, University of Isfahan, Hezar Jarib Street, Isfahan 81746-73441 (Iran, Islamic Republic of); Semsarha, Farid, E-mail: Semsarha@ibb.ut.ac.ir [Institute of Biochemistry and Biophysics (IBB), University of Tehran, P.O. Box: 13145-1384, Tehran (Iran, Islamic Republic of)

    2015-03-01

    In this study, simple single strand breaks (SSB) and double strand breaks (DSB) due to direct effects of the secondary electron spectrum of {sup 60}Co gamma rays on different organizational levels of a volume model of the B-DNA conformation have been calculated using the Geant4-DNA toolkit. Result of this study for the direct DSB yield shows a good agreement with other theoretical and experimental results obtained by both photons and their secondary electrons; however, in the case of SSB a noticeable difference can be observed. Moreover, regarding the almost constant yields of the direct strand breaks in the different structural levels of the DNA, calculated in this work, and compared with some theoretical studies, it can be deduced that the direct strand breaks yields depend mainly on the primary double helix structure of the DNA and the higher-order structures cannot have a noticeable effect on the direct DNA damage inductions by {sup 60}Co gamma rays. In contrast, a direct dependency between the direct SSB and DSB yields and the volume of the DNA structure has been found. Also, a further study on the histone proteins showed that they can play an important role in the trapping of low energy electrons without any significant effect on the direct DNA strand breaks inductions, at least in the range of energies used in the current study.

  4. Determination of the correction factor for attenuation, dispersion and production of electrons (Kwall) in the wall of graphite of a ionization chamber Pattern National Type CC01 in fields of gamma radiation of 60Co

    International Nuclear Information System (INIS)

    It was determined the Kwall correction factor for the wall of graphite of the chamber of the pattern national type CC01 series 133 for a radiation field Gamma of 60Co. With this end to measured the currents of ionization l(x) as function of the thickness of the wall of the chamber: X=4,8,12,16 and 20 mm.The mensurations for each thickness consisting of three groups, of sizes n = 30 or 60 data for each group; obtaining 8 complete groups of mensurations independent in eight different dates.The determinate the factor carried out using three regression models: lineal, logarithmic and quadratic, models that were tried to validate with the tests of : i) Shapiro-Wilk and χ2 for the normality of the entrance data ii) Tests of Bartlett for variances homogeneity among groups for each thickness iii) The tests of Duncan for the stockings among groups of each thickness, and iv) The tests of adjustment lack (LOF) for the models used. Nevertheless, alone the models of the group of corresponding mensurations at 01-03-2000 17-08-2001 they can be validated by LOF, but not for tests of normality and homogeneity of variances. Among other assignable causes of variation we have: i) The values captured by the system of mensuration of the variables of it influences: pressure, temperature and relative humidity dont belong together with the existent ones to the moment to capture the l(x). ii) The mensuration room presents flows of air, for what was suited o diminish their volume and to eliminate the flows of air. iii) A protocol settled down of taking of measures that it consisted in: - Pre-irradiation 5 minutes the chamber after the change of polarity and hood change, with a period of stabilization of 5 minutes after the pre-irradiation. - Pre-irradiation for 5 minutes before the taking of the readings, with the object of eliminating variation sources assigned to currents of escapes or due variations to transitory. iv) To realize corrections for relative humidity of agreement with the

  5. Identification of gamma-irradiated papaya, melon and watermelon

    International Nuclear Information System (INIS)

    Ionizing radiation can be used to control spoilage microorganisms and to increase the shelf life of fresh fruits and vegetables in replacement for the treatment with chemical fumigants. In order to enforce labelling regulations, methods for detecting the irradiation treatment directly in the produce are required. Recently, a number of detection methods for irradiated food have been adopted by the Codex Comission. A rapid screening method for qualitative detection of irradiation is the DNA Comet Assay. The applicability of the DNA Comet Assay for distinguishing irradiated papaya, melon, and watermelon was evaluated. The samples were treated in a 60Co facility at dose levels of 0.0, 0.5, 0.75, and 1.0 kGy. The irradiated samples showed typical DNA fragmentation whereas cells from non-irradiated ones appeared intact. In addition to the DNA Comet Assay also the half-embryo test was applied in melon and watermelon to detect the irradiation treatment

  6. Identification of gamma-irradiated papaya, melon and watermelon

    Energy Technology Data Exchange (ETDEWEB)

    Marin-Huachaca, N.S.; Mancini-Filho, Jorge E-mail: jmancini@usp.br; Delincee, Henry E-mail: henry.delincee@bfe.uni-karlsruhe.de; Villavicencio, A.L.C.H. E-mail: villavic@net.ipen.br

    2004-10-01

    Ionizing radiation can be used to control spoilage microorganisms and to increase the shelf life of fresh fruits and vegetables in replacement for the treatment with chemical fumigants. In order to enforce labelling regulations, methods for detecting the irradiation treatment directly in the produce are required. Recently, a number of detection methods for irradiated food have been adopted by the Codex Comission. A rapid screening method for qualitative detection of irradiation is the DNA Comet Assay. The applicability of the DNA Comet Assay for distinguishing irradiated papaya, melon, and watermelon was evaluated. The samples were treated in a {sup 60}Co facility at dose levels of 0.0, 0.5, 0.75, and 1.0 kGy. The irradiated samples showed typical DNA fragmentation whereas cells from non-irradiated ones appeared intact. In addition to the DNA Comet Assay also the half-embryo test was applied in melon and watermelon to detect the irradiation treatment.

  7. Identification of gamma-irradiated papaya, melon and watermelon

    Science.gov (United States)

    Marín-Huachaca, Nélida S.; Mancini-Filho, Jorge; Delincée, Henry; Villavicencio, Anna Lúcia C. H.

    2004-09-01

    Ionizing radiation can be used to control spoilage microorganisms and to increase the shelf life of fresh fruits and vegetables in replacement for the treatment with chemical fumigants. In order to enforce labelling regulations, methods for detecting the irradiation treatment directly in the produce are required. Recently, a number of detection methods for irradiated food have been adopted by the Codex Comission. A rapid screening method for qualitative detection of irradiation is the DNA Comet Assay. The applicability of the DNA Comet Assay for distinguishing irradiated papaya, melon, and watermelon was evaluated. The samples were treated in a 60Co facility at dose levels of 0.0, 0.5, 0.75, and 1.0kGy. The irradiated samples showed typical DNA fragmentation whereas cells from non-irradiated ones appeared intact. In addition to the DNA Comet Assay also the half-embryo test was applied in melon and watermelon to detect the irradiation treatment.

  8. Genotoxicity test of irradiated foods

    International Nuclear Information System (INIS)

    Safety tests of radiation irradiated foods started as early as from 1967 in Japan and genotoxicity tests in the Hatano Res. Inst., from 1977. The latter is unique in the world and is reviewed in this paper. Tests included those for the initial injury of DNA, mutagenicity, chromosomal aberration and transformation with use of bacteria, cultured mammalian cells and animals (for chromosomal aberration, micronucleus formation and dominant lethality). Foods tested hitherto were onion, rice, wheat and flour, Vienna sausage, fish sausage (kamaboko), mandarian orange, potato, black pepper and red capsicum, of which extract or powder was subjected to the test. Irradiation doses and its purposes were 0.15-6 kGy γ-ray (60Co) or electron beam by the accelerator (only for the orange), and suppression of germination, pesticide action or sterilization, respectively. Genotoxicity of all foods under tested conditions is shown negative. (N.I.)

  9. Optimization of point sources on thin films using a statistical design approach. Application to {sup 60}Co sources; Optimisation des sources ponctuelles sur film mince par une approche experimentale de type plan d'experiences. Application aux sources de {sup 60}Co

    Energy Technology Data Exchange (ETDEWEB)

    Sanoit, J.; Leprince, B. [CEA Saclay, Dept. d' Instrumentation et de Metrologie des Rayonnements Ionisants, 91 - Gif sur Yvette (France); Vailhen, D. [CEA Saclay, Dir. de la Surete Nucleaire et de la Qualite, 91 - Gif sur Yvette (France)

    2001-07-01

    Radioactive point sources prepared by depositing weighed drops on thin membranes are commonly used in ionizing radiation metrology for 47{pi}{beta}-{gamma} coincidence counting or for low energy electrons spectrometry. The present study deals with the preparation of good quality sources having high detection yields. Some influential parameters on the source quality are studied using {sup 60}Co as radioactive tracer. An approach based on experimental statistical designs has been chosen. This approach allows us to obtain reliable results with a number of experiences lower than in the case of a systematic approach. Homogeneous {sup 60}Co sources with high metrological qualifies (R{sub {beta}}> 96 %) can be prepared using a direct deposit of {approx} 5 mg drops of {sup 60}Co solution ([Co] = 12 {mu}g/g, [HCl] = 0,5 mol/I) and dried at 60 deg C. The support of the radioactive spot is constituted with a gold coated (130 {mu}g/cm{sup 2} of Au) Vyns films (thickness 45 {mu}g/cm{sup 2}) on which a pad (diameter = 7 - 8 mm) of polystyrene microspheres (Estapor K-007) is prepared by an electro-spraying technique under 7,5 kV during 10 minutes. The alcoholic solution used for the electro-spraying step has the following chemical composition [Estapor K-007] 2.5 10{sup -2} wt % and [Tween 20] = 2.7 10{sup -2} wt %. (authors)

  10. Cytogenetic and dosimetric effects of 131I in lymphocyte of patients with differentiated thyroid cancer with and without r-hTSH stimulation. Study in thyroid tumor cells (WRO) treated with 131I and 60Co in vitro

    International Nuclear Information System (INIS)

    , the cytotoxic assay, showed a tendency to decrease at higher concentrations of 1.85 (p <0.05) and 3.70 MBq/ml (p <0.01) only in the presence of r-hTSH, coincident with the highest level of uptake. WRO cells were also relatively radioresistant to external irradiation of 60Co in the range of 0.2-8.3 Gy, with a gradual decrease in function of time for higher doses (10,20 and 40Gy).The data obtained showed little cytogenetic damage in patients upon therapeutic exposure, suggesting a safe and effective treatment for both groups of patients. Patients in group A, however, had a better quality of life by using r-hTSH. In vitro studies with internal (131I) and external (60Co) irradiation, with or without r-hTSH, point to the need for an alternative therapeutic strategy to overcome the loss of ability of thyroid cells (WRO) to concentrate radioiodine, which is responsible for the failure of radioiodine therapy in patients with DTC. (author)

  11. Determination of the correction factor for attenuation, dispersion and production of electrons (K{sub wall}) in the wall of graphite of a ionization chamber Pattern National Type CC01 in fields of gamma radiation of {sup 60}Co; Determinacion del factor de correccion por atenuacion, dispersion y produccion de electrones (K{sub wall}) en la pared de grafito de una Camara de Ionizacion Patron Nacional Tipo CC01 en campos de radiacion gamma de {sup 60} Co

    Energy Technology Data Exchange (ETDEWEB)

    Alvarez R, J.T.; Morales P, J.; Cruz E, P. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)

    2001-12-15

    It was determined the Kwall correction factor for the wall of graphite of the chamber of the pattern national type CC01 series 133 for a radiation field Gamma of {sup 60}Co. With this end to measured the currents of ionization l(x) as function of the thickness of the wall of the chamber: X=4,8,12,16 and 20 mm.The mensurations for each thickness consisting of three groups, of sizes n = 30 or 60 data for each group; obtaining 8 complete groups of mensurations independent in eight different dates.The determinate the factor carried out using three regression models: lineal, logarithmic and quadratic, models that were tried to validate with the tests of : i) Shapiro-Wilk and {chi}{sup 2} for the normality of the entrance data ii) Tests of Bartlett for variances homogeneity among groups for each thickness iii) The tests of Duncan for the stockings among groups of each thickness, and iv) The tests of adjustment lack (LOF) for the models used. Nevertheless, alone the models of the group of corresponding mensurations at 01-03-2000 17-08-2001 they can be validated by LOF, but not for tests of normality and homogeneity of variances. Among other assignable causes of variation we have: i) The values captured by the system of mensuration of the variables of it influences: pressure, temperature and relative humidity don{sup t} belong together with the existent ones to the moment to capture the l(x). ii) The mensuration room presents flows of air, for what was suited o diminish their volume and to eliminate the flows of air. iii) A protocol settled down of taking of measures that it consisted in: - Pre-irradiation 5 minutes the chamber after the change of polarity and hood change, with a period of stabilization of 5 minutes after the pre-irradiation. - Pre-irradiation for 5 minutes before the taking of the readings, with the object of eliminating variation sources assigned to currents of escapes or due variations to transitory. iv) To realize corrections for relative humidity of

  12. Sensitivity of Listeria monocytogenes to irradiation

    International Nuclear Information System (INIS)

    Irradiation of Listeria monocytogenes (L.m.) was carried out in culture media and pork meat paste at room temperature with 60Co radiation source of 6.6 kGy h-1 dose rate. The employed doses were 0, 0.5, 1, 2, 3, 4 and 6 kGy. One strain out of 3 survived as high as 4 kGy irradiation. Radiation with 2 kGy resulted 7 log cycles reduction of cell count. After lower irradiation doses the L.m. count decreased in proportion to increasing doses. It has been concluded that L.m. compared with Gram-negative pathogens, are less sensitive to irradiation. (author) 6 refs.; 4 figs

  13. Thermoluminescence properties of irradiated chickpea and corn

    Science.gov (United States)

    Necmeddin Yazici, A.; Bedir, Metin; Bozkurt, Halil; Bozkurt, Hüseyin

    2008-02-01

    A study was carried out to establish a detection method for irradiated chickpea and corn by thermoluminescence (TL) method. The leguminous were packed in polyethylene bags and then the packets were irradiated at room temperature at different doses by 60Co gamma source at 1, 4, 8 and 10 kGy. Minerals extracted from the leguminous were deposited onto a clean aluminum disc and TL intensities of the minerals were measured by TL. It was observed that the extracted samples from both leguminous exhibit good TL Intensity and the TL intensity of glow curves of them increased proportionally to irradiation doses. The TL glow curve of both irradiated leguminous presents a single broad peak below 400 °C. The TL trapping parameters glow peaks were estimated by the additive dose (AD), Tm(Ea)-Tstop and computerized glow curve deconvolution (CGCD) methods. The fading characteristics of glow curves were also recorded up to 6 months.

  14. Microbial determination of Cumin by gamma irradiation

    International Nuclear Information System (INIS)

    Cumin is one of the valuable export items of Iran, and like most of the agricultural products it is contaminated by microorganisms. Due to importance of this product, the gamma irradiation method, which has applications in microbial decontamination, has been used for the improving its quality and increasing the shelf life-time. For this purpose pak ages of 10 gr of cumin were irradiated by 2,4,6 and 8 KGy from 60Co source. With each dose, four samples were irradiated and results were compared with controlled not irradiated samples. According to the standard limitation of bacteria and molds the total optimum doses are 7.5 and 5 KGy respectively

  15. Effect of gamma radiation from {sup 60}C in conservation and quality of pepper fresh and pulp; Efeito da radiacao gama do {sup 60}Co na conservacao e qualidade de pimenta in natura e em polpa

    Energy Technology Data Exchange (ETDEWEB)

    Milagres, Regina Celia Rodrigues de Miranda

    2014-07-01

    Capsicum peppers are among the most used and most valued seasoning spices in the world. They are highly susceptible to post harvest decay, therefore the use of irradiation may contribute to conservation of this fruit which has significant nutritional, economic and social value. Were evaluated the effects of gamma radiation of {sup 60}Co in increasing the shelf-life and preservation of quality of pepper 'Dedo-de-moca' (Capsicum baccatum var. Pendulum) fresh and pulp associated or not with another conservation methods. Gamma radiation doses were investigated of 0,25; 0,50; 0,75; 1,00; 1,25; 1,50; 2,00 e 3,00 kGy in fresh fruit and 1,50; 2,00; 3,00; 4,50 e 6,00 kGy in pepper pulp. The use of laboratory pasteurization (85°C/3min), the addition of citric acid (5%) and 5% NaCl were also investigated in the pepper pulp. The samples were stored at 5 °C and or 25 °C. Were performed analysis: visual (disease incidence, turgidity and color); bioactive compounds (carotenoids, capsaicinoids, antioxidant capacity and phenolic compounds); physicochemical (Weight loss, firmness, soluble solids (SS), pH, titratable acidity (TA), ratio, color and moisture); respiratory rate and ethylene production; proximate composition (moisture, soluble and insoluble fiber, ash, protein, ether extract, total and available carbohydrate); microbiological contamination (mesophilic aerobic microorganisms, coliforms at 45 °C, Salmonella spp. and yeasts and molds) and sensory (acceptance test, difference test and paired preference). Radiation doses between 0.25 and 3.00 kGy were not effective to increase shelf-life and maintain fresh pepper quality during storage. There was increased intensity of red color, of incidence of disease, decreased turgidity and changes in physical, chemical and nutritional parameters. In pulp, the doses of gamma radiation of 4.50 and 6.00 kGy and the addition of 5% NaCl no changing sensory attributes, physical-chemical and bioactive compounds. Only the

  16. Dose-dependent and gender-related radiation-induced transcription alterations of Gadd45a and Ier5 in human lymphocytes exposed to gamma ray emitted by 60Co

    International Nuclear Information System (INIS)

    Growth arrest DNA damage-inducible 45a gene (Gadd45a) and immediate early response gene 5 (Ier5) have been emphasised as ideal radiation bio-markers in several reports. However, some aspects of radiation-induced transcriptional alterations of these genes are unknown. In this study, gender-dependency and dose-dependency as two factors that may affect radiation induced transcription of Gadd45a and Ier5 genes were investigated. Human lymphocyte cells from six healthy voluntary blood donors (three women and three men) were irradiated in vitro with doses of 0.5-4.0 Gy from a 60Co source and RNA isolated 4 h later using the High Pure RNA Isolation Kit. Dose and gender dependency of radiation-induced transcriptional alterations of Gadd45a and Ier5 genes were studied by quantitative real-time polymerase chain reaction. The results showed that as a whole, Gadd45a and Ier5 gave responses to gamma rays, while the responses were independent of radiation doses. Therefore, regardless of radiation dose, Gadd45a and Ier5 can be considered potential radiation bio-markers. Besides, although radiation-induced transcriptional alterations of Gadd45a in female and male lymphocyte samples were insignificant at 0.5 Gy, at other doses, their quantities in female samples were at a significantly higher level than in male samples. Radiation induced transcription of Ier5 of females samples had a reduction in comparison with male samples at 1 and 2 Gy, but at doses of 0.5 and 4 Gy, females were significantly more susceptible to radiation-induced transcriptional alteration of Ier5. (authors)

  17. Properties of Pulsed Photostimulated Luminescence and Thermoluminescence for Detection of Gamma-Irradiated Teas during Storage

    International Nuclear Information System (INIS)

    Green, black and oolong teas were irradiated by @@€@@60Co-gamma rays (0~10 kGy) and were investigated for detection of irradiation treatment using pulsed photostimulated luminescence (PPSL) and thermoluminescence (TL) during storage. Teas irradiated at 2.5 kGy or more showed a photon count of greater than 5000 counts/60 sec while the non-irradiated yielded only 650~1000 count/60 sec. Correlation coefficients between irradiation dose and photon counts/60 sec were 0.8951, 0.7934 and 0.9007 for green, black and oolong teas, respectively

  18. Cytogenetic and dosimetric effects of {sup 131}I in lymphocyte of patients with differentiated thyroid cancer with and without r-hTSH stimulation. Study in thyroid tumor cells (WRO) treated with {sup 131}I and {sup 60}Co in vitro; Efeitos citogenetico e dosimetrico do {sup 131}I em pacientes com cancer diferenciado da tireoide com e sem estimulacao com r-hTSH. Estudo em celulas tumorais tireoidianas (WRO) tratadas com {sup 131}I e {sup 60}Co in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Valgode, Flavia Gomes Silva

    2015-11-01

    significance, with or without r-hTSH. However, the cytotoxic assay, showed a tendency to decrease at higher concentrations of 1.85 (p <0.05) and 3.70 MBq/ml (p <0.01) only in the presence of r-hTSH, coincident with the highest level of uptake. WRO cells were also relatively radioresistant to external irradiation of {sup 60}Co in the range of 0.2-8.3 Gy, with a gradual decrease in function of time for higher doses (10,20 and 40Gy).The data obtained showed little cytogenetic damage in patients upon therapeutic exposure, suggesting a safe and effective treatment for both groups of patients. Patients in group A, however, had a better quality of life by using r-hTSH. In vitro studies with internal ({sup 131}I) and external ({sup 60}Co) irradiation, with or without r-hTSH, point to the need for an alternative therapeutic strategy to overcome the loss of ability of thyroid cells (WRO) to concentrate radioiodine, which is responsible for the failure of radioiodine therapy in patients with DTC. (author)

  19. Study of {sup 137}Cs e {sup 60}Co sorption in sediments from Saco de Piraquara de Fora, Angra dos Reis and its application for environmental monitoring; Estudo da sorcao de {sup 137}Cs e {sup 60}Co em sedimentos do Saco de Piraquara de Fora em Angra dos Reis e sua aplicacao para o monitoramento ambiental

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, Ingryd Marques

    2016-08-01

    Both the suspended solid particles and the bed sediments sorb radionuclides, released in water systems. Sorption is usually represented mathematically by the distribution coefficient that is based in equilibrium between phases. Here the adsorption/desorption kinetics of {sup 60}Co and {sup 137}Cs in sea water were simulated by batch equilibrium experiments with sediments in two points (PT - 01, PT - 02) from Saco de Piraquara de Fora inlet (SPF). For both radionuclides, partition coefficient values (Kd) for the sampling point PT - 02 (509 and 385 L/kg for {sup 60}Co and {sup 137}Cs, respectively) were higher than the values determined for PT - 01 one (426 and 182 L/kg for {sup 137}Cs and {sup 60}Co, respectively). The higher values of Kd of PT-02 reflects the higher CEC (71,4 cmolc.dm{sup 3}) and content of mud < 63 μm (87%) when compared to PT-01 (CEC of 39,5 cmol{sub c}.dm{sup 3}) and mud (55%). In comparison with the values reported in the literature, the found Kd values are low, which may be related to the predominance of kaolinite, which is a clay of low sorption capacity. The Kd values with an increase in temperature of 23 deg C to 27 deg C were similar ({sup 60}Co in PT-02 and {sup 137}Cs in both sediment) or 27 ° C values were higher ({sup 60}Co the PT-01). With increasing temperature to 31 °C Kd values for the two radionuclides showed a decrease. However, increasing temperature increases the desorption of the two radionuclides for both sediments The sorption process is spontaneous and favorable for both sediments and the model of sorption can be fitted by both Freundlich and Langmuir sorption isotherms. The maximum amount of {sup 60}Co that can be sorbed on sediments were 1,64 10{sup -5} moles/g (4,12 10{sup 10} Bq/g) and 2,79 10{sup -5} moles/g (7,03 10{sup 10} Bq/g) to PT-01 and PT-02, respectively, and of {sup 137}Cs 1,99 10{sup -6} moles/g (9,70 10{sup 8} Bq/g) and 6,60 10{sup -6} mol/g (2,87 10{sup 9} Bq/g ) to PT-01 and PT-02. Two areas in SPF can

  20. Change in the amino acid composition of calf skin collagen after. gamma. -irradiation in aqueous solution

    Energy Technology Data Exchange (ETDEWEB)

    Duzhenkova, N.A.; Savich, A.V. (Institut Biofiziki, Moscow (USSR))

    A study was made of the amino acid composition of calf skin collagen after ..gamma..-irradiation (/sup 60/Co) of 2.5x10/sup -6/ M aerated aqueous protein solution within the dose range from 30 to 2000 Gy. The radiosensitivity of amino acid residues was compared.

  1. Study on mutagenic effects of rice seeds irradiated by CO2 laser

    Institute of Scientific and Technical Information of China (English)

    LUORongting; ZHANGMingring

    1998-01-01

    Seeds of japonica rice (cv. Zhenuo 2) at two different physiotogical states (dry seeds with water content 13% and wet seeds soaked inthe water for 36 h) were irradiated by CO2 laser in four different power-densities. The seeds rradiated by 200GY of 60Co γ rays and no radiated seeds were used as the controls.

  2. Irradiation effects on meat: a review Efeito da irradiação na carne: uma revisão

    OpenAIRE

    Fábio Costa Henry

    2009-01-01

    Food irradiation is a process exposing food to ionizing radiations such as gamma rays emitted from the radioisotopes 60Co and 137Cs, or, high energy electrons and X-rays produced by machine sources. Irradiation can induce formation of isooctane-soluble carbonyl compounds in the lipid fraction and acid-soluble carbonyls in the protein fraction of meat. Increasing irradiation dose increases these compounds however, cooking reduces them. Among the volatile components, 1-heptene and 1-nonene are ...

  3. Key comparison BIPM.RI(I)-K1 of the air-kerma standards of the NIM, China and the BIPM in 60Co gamma radiation

    Science.gov (United States)

    Kessler, C.; Burns, D.; Wang, K.; Fan, Y.; Jin, S.; Yang, X.

    2016-01-01

    An indirect comparison of the standards for air kerma of the National Institute of Metrology (NIM), China and of the Bureau International des Poids et Mesures (BIPM) was carried out in the 60Co radiation beam of the BIPM in November 2015. The comparison result, evaluated as a ratio of the NIM and the BIPM standards for air kerma, is 0.9997 with a combined standard uncertainty of 2.7 × 10-3. The results are analysed and presented in terms of degrees of equivalence for entry in the BIPM key comparison database. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCRI, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  4. Preparation of Plate Fe60Co8Zr10Mo5W2B15 Bulk Amorphous Alloy and Its Fracture Toughness

    Institute of Scientific and Technical Information of China (English)

    XIAO Huaxing; CHEN Guang

    2005-01-01

    With processes of arc melting, inductive melting and copper mold suction casting, a plate Fe-based bulk amorphous alloy Fe60Co8Zr10Mo5W2B15 with a thickness of 1mm was prepared. The surfaces and fractures of the cast bulk amorphous alloy were agleam and with typical metallic luster. The glass transition temperature(Tg), supercooled liquid region(△Tx)and reduced glass transition temperature(T rg)of the prepared Fe-based amorphous alloy are 884 K,63 K, and 0.611 respectively. The fracture toughness of the cast bulk amorphous alloy is at the level of 1.6 MPa·m1/2.

  5. Morphology and properties of polyurethane/poly(ST) or poly(MMA) interpenetrating polymer networks by 60Co γ-ray

    International Nuclear Information System (INIS)

    Interpenetrating polymer networks (IPNs) are the mixture of two or more crosslinked polymers which are dispersed or mixed in the segmental level. In general, due to the low entropy of mixing, segmentally dispersed networks are difficult to be realized. It was able to increase further the degree of interpenetration in the incompatible PU-PMMA IPN by applying 60Co-γ radiation polymerization. By using the simultaneous polymerization process under radiation, a transparent, nearly segmentally dispersed mixture of the IPN was able to be obtained. The study on the IPN by small angle X-ray scattering and transmission electron microscopy showed that radiation-initiated polymerization is an effective means for obtaining the PU-PMMA IPN with high extent interpenetration. The IPN with two continuous phase structure was obtained. The materials used for the experiment, the method of synthesis, and the results of the morphology, glass transition and mechanical properties of the IPN are reported. (K.I.)

  6. Application of the Cavity theory in the calibration of the powder TLD-100 for energies of 60 Co, 137 Cs, 192 Ir and RX 50, 250 k Vp

    International Nuclear Information System (INIS)

    A powder lot TLD-100 (LiF:Mg,Ti) in absorbed dose terms in water Dw for the following radiation sources: 60 Co, 137 Cs and RX 50 and 250 k Vp is calibrated; to continuation is made a lineal interpolation of the TLD response in function of the effective energy of the sources to calibrate a source of 192 Ir. The calibration of those fields in Dw are carried out with aid of the Bragg-Gray cavity theory, the one which finds implicit in the following protocols: IAEA-TRS 398 for the 60 Co and the AAPM TG61 for X Rays of 50 and 250 k Vp. Additionally the AAPM protocol TG43 to determine the Dw in function of the kerma intensity Sk in the case of the 137 Cs is used. The calibration curves for the response of the TLD-100 RTLD vs Dw, corresponding to each one of the sources already mentioned are constructed. The RTLD vs Dw by least heavy square by means of a second order polynomial that corrects the supralineality of the response is adjusted. The curves are validated by lack of LOF adjustment and by the Anderson Darling normality test. Later the factors of sensitivity (Fs) for the sources of 192 Ir: Micro Selectron and Vari Source are interpolated, used respectively in the A and B hospitals for treatments of brachytherapy of high dose rate (HDR), the expanded uncertainties associated to the Dw and Fs are also determined. Finally, an acrylic phantom and a couple of capsules are already sent to the hospitals mentioned, to verify a nominal Dw of 2 Gy, in a case an underestimate in 5.5% in the imparted Dw and in other an overestimation in a range of -1.5 to -8.0% was obtained. The obtained results in this work establish the bases for the development of a national dosimetric quality control program for brachytherapy of HDR with sources of 192 Ir. (Author)

  7. Diagnosis Molekuler Toxoplasma gondii Berdasar Gen Stage Spesifik Takizoit dan Bradizoit pada Ayam Kampung (MOLECULAR DIAGNOSIS OF TOXOPLASMA GONDII BASED ON THE TACHYZOITE AND BRADYZOITE STAGE SPECIFIC GENES IN FREE-RANGE CHICKEN

    Directory of Open Access Journals (Sweden)

    Ida Ayu Pasti Apsari

    2014-04-01

    Full Text Available The aims of this study was to determine the presence of Toxoplasma gondii in free-rangechicken using Polymerase Chain Reaction (PCR method based on the tachyzoite and bradyzoitestage specific genes. SAG1 and BAG1 are the tachyzoite and bradyzoite stage-specific gene respectively.The primers for SAG1 and BAG1 were designed using Web-base Program Primer 3. Genomic DNAfrom free-range chicken heart and brain was isolated using Pure-Link Genomic Isolation Kit.DNA amplification by PCR using primers for SAG1 and BAG1 genes was used for diagnosis ofT.gondii. The results showed that the DNA amplification using primers for SAG1 and BAG1 geneswas successfully applied to determine of Toxoplasma gondii in free-range chicken.

  8. Social and environmental aspects of a sewage sludge irradiation plant

    International Nuclear Information System (INIS)

    The critical environmental parameters involved in an environmental impact study for a 700,000 Ci of 60Co sewage sludge irradiation plant are described and analyzed. The plant is the first that will operate in Argentina and it is located in a town of 500,000 inhabitants, in an agricultural region with no nuclear tradition. The position of the environmental authorities and of the public opinion is analyzed. Possible information alternatives are proposed. (author)

  9. 10例60Co源辐射事故受照者眼晶状体的随访观察%Follow-up observation of eye lens in ten victims accidentally exposed to 60Co radiation source

    Institute of Scientific and Technical Information of China (English)

    赵风玲; 陈玉浩; 刘金星; 吕玉民; 傅宝华

    2015-01-01

    目的 观察60Co源辐射事故对受照者眼晶状体的远后效应.方法 对河南省1986年至2000年期间发生的4起60Co源辐射事故共10例受照者进行眼晶状体远期医学观察.用复方托吡卡胺充分散瞳后在裂隙灯下检查眼晶状体,记录病变特征.结果 开封事故中"亮"、新乡事故中"梅"分别于照后2、3年出现典型放射性白内障;许昌事故中"许",照后6年出现典型放射性白内障;开封事故中"燕"、郑州事故中"杰"、新乡事故中"天"、"勇"、"义"照后随访观察仅出现双眼晶状体后囊下少量点状、颗粒状浑浊等放射性白内障的初期改变,未观察到典型放射性白内障的形态特点;新乡事故中"旺"、"民"则未观察到晶状体后囊下的浑浊.结论 眼晶状体是辐射事故受照者远期随访观察应重点关注的靶器官.电离辐射致眼晶状体浑浊的发展程度与照射剂量密切相关.%Objective To observe late effect of 60Co radiation accident on eye lens in the victims.Methods Medical observations of eye lens were performed on ten victims accidentally exposed to 60Co sources in four radiation accidents that have occurred from 1986 to 2000 in Henan Province.Pathological changes of the eye lenses were examined by using slit lamp microscopy after mydriasis with compound tropicamide.Results Of these ten victims, Liang in a radiation accident in Kaifeng (in 1986), Mei in Xinxiang (in 1999) and Xu in Xuchang (in 2000) all had typical radiation-induced cataract 2, 3 and 6 years after irradiation, respectively.Follow-up observation of the lens showed the punctate and/or granular opacities present in the eye posterior subcapsular of Yan in Kaifeng accident, Jie in Zhengzhou (1987), and Tian, Yong and Yi in Xinxiang accident, featuring the early changes of radiation-induced cataract, but the posterior subcapsular opacities were not observed in Wang and Min in Xinxiang accident.Conclusions Focus should be on the eye lens as the

  10. The Effect of Vitamin D3 Alone and Mixed with IFN-γ on Tachyzoites of Toxoplasma Gondii (RH Strain Proliferation and Nitric Oxide (NO Production in Infected Macrophages of BALB/C Mice

    Directory of Open Access Journals (Sweden)

    E Al-Kawaz

    2010-09-01

    Full Text Available Introduction: Toxoplasma gondii is an obligatory interacelullar parasite that infects nucleated cells in its intermediate hosts. The aim of the present study was to determine the effect of vitamin D3 on the multiplication of T. gondii in peritoneal macrophage of Balb/c mice and nitric oxide production by macrophages. Methods: According to usage of vitamin D3 (one dose or seven doses and INFγ in vitro and in vivo, this study was divided into four experiments. In all experiments, the macrophages were col­lected from peritoneum and cultured in RPMI-1640. Then the supernatants were collected after 24 h and their nitric oxide was measure. After 96 h, the macrophages were collected and stained and the number of tachyzoites was measured. Results: The first experiment (the mice were infected with tachyzoites and after 2 h, got one dose vita­min D3 intraperitonealy showed the best results. The mean of tachyzoites per macrophages was 2.37, and mean ± SD of nitric oxide was 187.8 ± 9.Discussion: High-level production of nitric oxide may be related to the only one injection of vita­min D3. The injection in long time might suppress the immune system.

  11. Effect of irradiation on seed germination and seedling growth of Pinus armandi Franch

    International Nuclear Information System (INIS)

    The soaking seeds of Pinus armandi Franch. were irradiated with 60Co γ-ray at dose of 0, 20, 40, 60 and 80 Gy, and the treated seeds were planted. The result showed that the irradiation treatment retarded the time of germination, and the seed germination rate, for reduced, the ratio of yellow to living seedlings. The treatment also lowered the height of seeding, especially for the yellow seedlings. (authors)

  12. Low dose gamma irradiation has no effect on the functionality of fi