Arildo José Braz de Oliveira; Vanda Marilza de Carvalho; Alexandre Ferreira; Fernando Y. Sato; Maria de Fátima Pires da Silva Machado
This study describes a simple and promising for in vitro multiplication of Tabernaemontana fuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source of compounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MS medium containing different concentrations of the cytokinins benzylaminopurine (BA) and 6-furfurylaminopurine (kinetin), supplemented with phloroglucinol (1, 3, 5-hydroxybenzene) to sti...
Axelrod, Felicia B.; Liebes, Leonard; Gold-von Simson, Gabrielle; Mendoza, Sandra; Mull, James; Leyne, Maire; Norcliffe-Kaufmann, Lucy; Kaufmann, Horacio; Slaugenhaupt, Susan A.
Familial dysautonomia (FD) is caused by an intronic splice mutation in the IKBKAP gene that leads to partial skipping of exon 20 and tissue-specific reduction in I-κ-B kinase complex associated protein/ elongation protein 1 (IKAP/ELP-1) expression. Kinetin (6-furfurylaminopurine) has been shown to improve splicing and increase wild-type IKBKAP mRNA and IKAP protein expression in FD cell lines and carriers. To determine if oral kinetin treatment could alter mRNA splicing in FD subjects and was...
Marcelina Vélez Torres
Full Text Available La regeneración de plantas haploides, es una herramienta importante en los programas de mejoramiento y estudios genéticos, ya que permite obtener líneas puras más rápido que los métodos convencionales a través de la duplicación de plantas haploides. El objetivo de este trabajo fue establecer una metodología que permita la regeneración de plantas haploides de chile tipo miahuateco (Capsicum annuum L.. Las anteras se cultivaron en los medios basales de Murashige y Skoog (1962; Chu et al. (1975, suplementados con 6-furfurilaminopurina (0.1-1 mg L-1, ácido naftalenacético (0.1 mg L-1, ácido indolacético (1 mg L-1 y ácido 2-4 diclorofenoxiacético (1 mg L-1. La embriogénesis se indujo hasta en 2.23% de anteras cuando se cultivaron en una combinación de 6-furfurilaminopurina con 2-4, diclorofenoxiacético (1 mg L-1 de ambos o de ácido indolacético con 6-furfurilaminopurina (0.1 mg L-1 de ambos. El análisis cromosómicos de las plantas regeneradas mostró que eran haploides con número cromósomico 2n= x= 12.Haploid plant regeneration is an important tool in breeding programs and genetics studies, since it helps obtain pure lines faster than conventional methods by the duplication of haploid plants. The aim of this study was to establish a methodology to regenerate haploid Miahuateco chili pepper plants (Capsicum annuum L.. Anthers were grown on Murashige and Skoog (1962; Chu et al. (1975 basal media, supplemented with 6-furfurylaminopurine (0.1-1 mg L-1, naphthaleneacetic acid (0.1 mg L-1, indolacetic acid (1 mg L-1, and 2-4 dichlorophenoxyacetic acid (1 mg L-1. Embryogenesis was induced in 2.23% of anthers grown in a combination of 6-furfurylaminopurine with 2-4 dichlorophenoxyacetic acid (1 mg L-1, of each, or indolacetic acid with 6-furfurylaminopurine (0.1 mg L-1 of each. Chromosome analysis of regenerated plants showed that they were haploids with a chromosome number of 2n= x= 12.
Full Text Available Rhizome cultures of "Jiianita" Polish cultivar of Alstroemeria x hybrida were used to enhance an effectiveness of micropropagation method of new cultivars and selections. The effect of cytokinins (BAP. kinetin and 2iP, auxins (IAA, IBAand NAA, growth retardants (paclobutrazol and flurprimidol alone or in combination were studied in relation to rhizome branching. aerial shoot production and rooting of rhizome. The greatest number of aerial shoots as well as the shortest shoots were observed at the highest BAP concentration (6 mg l-1. However, the rhizonies had the poorest rooting ability. BAP at low concentrations combined with kinetin or 2iP also strongly stimulated aerial shoot formation and rhizome branching. Unfortunately. those shoots were of poor qualily. Application of BAP at low concentration with paclobutrazol (0,1-0,5 mg l-1 or flurprimidol (0,01- 1 mg l-1 in presence of 1 mg l-1 NAA resulted in high number of aerial shoots (5-6, reduction of their length and higher rooting ability of the rhizomes. Gr(wth retardants applied with NAA strongly stimulated formation of the roots but suppressed their elongation. Abbreviations: BAP - 6=benzylaminopurine; kinetin - 6-furfurylaminopurine; 2iP - 6-γ,γ-dime-thylallylamino]purine; IAA-indole-3-acetic acid; IBA-indole-3-butyric acid; NAA- naphthaleneacetic acid; paclobutrazol (ICI PP-333 - (2-RS,3-RS-1-(4-chlorophenyl-4-4-dimethyl-2(1,2,3-triazol-1-yl-pentan-3-ol flurprimidol (Dowelanco - α-(1-niethylethy 1-α-[4-trifluro-niethoxyphenyl]-5-pyridinemethanol.
Callus cultures of jatropha (Jatropha curcas L.) grown in Murashige and Skoog's (MS) medium supplemented with naphthalene-acetic acid (NAA, 20 microM) and 6-furfurylaminopurine (kinetin, 20 microM) were exposed to ultraviolet-B (UV-B) radiation to investigate its potential as an abiotic elicitor of flavonoid production. Prior to irradiation, the levels of the flavonoids, apigenin, vitexin and isovitexin in the leaf and callus extracts were determined through high performance liquid chromatography (HPLC). Results showed that vitexin and isovitexin were the dominant flavonoids in the leaves while only apigenin was detected in the calli, suggesting a correlation between the degree of differentiation and biosynthesis of flavonoids in plant tissues. Irradiation of callus cultures for 7 d using two UV-B doses (12.6 and 25.3 kJ/sq m) induced synthesis of all three flavonoids (up to 780 micro g/g dw increase) to levels similar to or higher than those found in whole leaves. The combined levels of the three flavonoids in the cultures treated with the higher UV-B dose were 20-fold higher than the control and were comparable to concentrations found in leaves while a 10-fold increase in combined flavonoid levels was observed in calli irradiated with the lower UV-B dose. Furthermore, random amplified polymorphic DNA (RAPD) analyses of DNA extracts from the leaves and calli revealed that UV-B irradiation enhanced flavonoid synthesis without altering DNA sequence. These results further support the supposed involvement of UV-B in the transcriptional regulation of the expression of flavonoid biosysnthetic genes. Overall, the findings showed that elicitation through UV-B irradiation is an effective strategy to induce flavonoid production in dedifferentiated J. curcas cultures that have lost their capacity to produce the flavonoids normally synthesized in intact organs. (author)
Balestri, Elena; Vallerini, Flavia; Castelli, Alberto; Lardicci, Claudio
Exogenous application of plant growth regulators (PGRs) may be an effective technique for increasing the rooting ability and the growth of vegetative fragments (cuttings) of plants used in dune restoration programs. Various concentrations (0, 50 and 100 mg l-1) of two auxins, alpha-naphtaleneacetic acid (NAA) and indole-3-butyric acid (IBA), and two cytokinins, 6-furfurylaminopurine (Kinetin) and 6-benzylaminopurine (BAP), were applied separately to cuttings of two widely used species for restoration, Ammophila arenaria and Sporobuls virginicus. Root development and production of new buds in cuttings were examined under laboratory conditions one month after application. Cuttings were also examined one year after transplanting into a sandy substratum under natural conditions, to test for possible long term effects of PGRs on plant establishment success and growth. The response of the two study species to PGRs differed substantially. In A. arenaria the auxin NAA at 100 mg l-1 reduced the time for root initiation and increased the rooting capacity of cuttings, while the cytokinin Kinetin at 50 mg l-1 facilitated root growth. No auxin had effect on rooting or growth of S. virginicus cuttings, but treatment with 100 mg l-1 Kinetin resulted in higher rooting success than the control. One year after planting, the cuttings of A. arenaria treated with 100 mg l-1 NAA showed a higher establishment success (90% vs. 55%) and produced more culms and longer roots than the control; those treated with cytokinins did not differ in the establishment success from the control, but had longer roots, more culms and rhizomes. On the other hand, the cuttings of S. virginicus treated with 100 mg l-1 Kinetin showed a higher establishment success (75% vs. 35%) and had more culms than the control. Therefore, in restoration activities that involved A. arenaria, a pre-treatment of cuttings with NAA would be beneficial, as it allows the production of a higher number of well-developed plants with
Norton Borges Júnior
Full Text Available A acácia-negra (Acacia mearnsii De Wild. é uma espécie de difícil micropropagação devido à pequena capacidade de multiplicação e desenvolvimento de gemas. O presente estudo visou determinar a influência de diferentes citocininas na proliferação de gemas axilares em segmentos nodais de A. mearnsii. Plântulas germinadas in vitro forneceram explantes que foram inoculadas em meio básico B5 (GAMBORG et al., 1968. Testaram-se as citocininas: BAP (6-benzilaminopurina, BA (6-benziladenosina, 2iP (gama,gama-isopenteniladenina e cinetina (6-furfuralamino-purina. Diferentes concentrações desses reguladores de crescimento foram empregadas: 1 mgL-1, 2 mgL-1 e 3 mgL-1. Utilizou-se o delineamento de blocos casualizados, em arranjo fatorial, com seis repetições e cinco plantas por parcela. As avaliações foram feitas aos 30 dias, através da contagem de gemas alongadas e da presença de calos. A utilização de BAP a 2 mgL-1 promoveu a maior taxa de multiplicação de gemas (3,5 brotos/explante.Black wattle (Acacia mearnsii de Wild. is difficult to micropropagate due to the low ability of multiplication and development of shoots. Thus, the present study aimed at determining the influence of various cytokinins on axillary bud proliferation in nodal segments of A. mearnsii. Explants from in vitro germinated seedlings were inoculated on B5 (Gamborg et al., 1968 basal medium. BAP (6-benzylaminopurine, BA (6-benzyladenine, 2iP (gamma,gamma-dimethylallylamino-purine and Kinetin (6-furfurylaminopurine were tested at the concentrations 1 mgL-1, 2 mgL-1, and 3 mgL-1. A randomized block design, in factorial arrangement with 6 replications, and 5 plants per plot was used. The assessments were made after 30 days, by counting the elongated shoots and the presence of callus. The use of BAP at 2 mgL-1 promoted the highest rate of bud multiplication (3,5 shoots/explant.
Arildo José Braz de Oliveira
Full Text Available This study describes a simple and promising for in vitro multiplication of Tabernaemontana fuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source of compounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MS medium containing different concentrations of the cytokinins benzylaminopurine (BA and 6-furfurylaminopurine (kinetin, supplemented with phloroglucinol (1, 3, 5-hydroxybenzene to stimulate growth and shoot proliferation. Cytokinin added to the culture media positively influenced the micropropagation of T. fuchsiaefolia.and kinetin induced more shoots per explant than BA cytokinin. A favorable effect of phloroglucinol on apical and lateral buds from hypocotyls was also achieved in medium containing no kinetin or in all kinetin concentrations tested. Short pulses of auxin 3-indolebutyric acid (IBA 5.0 mg/l resulted in satisfactory rooting in apical microcuttings. The addition of phloroglucinol to MS medium induced rhizogenesis in 29% of the nodal segments transferred to MS medium in the absence of IBA and in 50% of the nodal segments transferred to MS medium containing 0.5 mg/l IBA and in nodal segments previously submitted to short pulses of IBA.O presente estudo relata um método simples e promissor para multiplicação in vitro de Tabernaemontana fuchsiaefolia, uma espécie abundantemente encontrada no sul do Brasil, utilizada com propósitos medicinais e como fonte de compostos que podem ser usados para desenvolver novas drogas sintéticas. Para estimular o crescimento e a proliferação de caules, os explantes apicais e os hipocótilos foram cultivados em meio MS contendo diferentes concentrações das citocininas 6-benzil-amino-purina (BA e cinetina (KIN e suplementado com fluoroglucinol (1, 3, 5-hidroxibenzeno. A micropropagação de T. fuchsiaefolia foi positivamente influenciada por citocininas e a cinetina induziu à formação de