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Sample records for 3t intera magnetic

  1. A 3 T magnet system for MAGNUM-PSI

    NARCIS (Netherlands)

    Eck, van H.J.N.; Ouden, den A.; Rooij, van G.J.; Goedheer, W.J.; Groot, de B.; Lopes Cardozo, N.J.; Kleyn, A.W.

    2005-01-01

    The FOM-Institute for Plasma Physics is preparing the construction of Magnum-psi, a magnetized (3 T), steady-state, large area (100 cm2), high-flux (up to 1024 H+ ions m-2s-1) plasma generator. Magnum-psi will be used to study plasma-surface interaction in conditions similar to those in the divertor

  2. Malformations of cortical development: 3T magnetic resonance imaging features

    Science.gov (United States)

    Battal, Bilal; Ince, Selami; Akgun, Veysel; Kocaoglu, Murat; Ozcan, Emrah; Tasar, Mustafa

    2015-01-01

    Malformation of cortical development (MCD) is a term representing an inhomogeneous group of central nervous system abnormalities, referring particularly to embriyological aspect as a consequence of any of the three developmental stages, i.e., cell proliferation, cell migration and cortical organization. These include cotical dysgenesis, microcephaly, polymicrogyria, schizencephaly, lissencephaly, hemimegalencephaly, heterotopia and focal cortical dysplasia. Since magnetic resonance imaging is the modality of choice that best identifies the structural anomalies of the brain cortex, we aimed to provide a mini review of MCD by using 3T magnetic resonance scanner images. PMID:26516429

  3. Malformations of cortical development:3T magnetic resonance imaging features

    Institute of Scientific and Technical Information of China (English)

    Bilal; Battal; Selami; Ince; Veysel; Akgun; Murat; Kocaoglu; Emrah; Ozcan; Mustafa; Tasar

    2015-01-01

    Malformation of cortical development(MCD) is a term representing an inhomogeneous group of central nervous system abnormalities, referring particularly to embriyological aspect as a consequence of any of the three developmental stages, i.e., cell proliferation, cell migration and cortical organization. These include cotical dysgenesis, microcephaly, polymicrogyria, schizencephaly, lissencephaly, hemimegalencephaly, heterotopia and focal cortical dysplasia. Since magnetic resonance imaging is the modality of choice that best identifies the structural anomalies of the brain cortex, we aimed to provide a mini review of MCD by using 3T magnetic resonance scanner images.

  4. Thirty-two-channel coil 3T magnetic resonance-guided biopsies of prostate tumor suspicious regions identified on multimodality 3T magnetic resonance imaging: technique and feasibility.

    NARCIS (Netherlands)

    Hambrock, T.; Futterer, J.J.; Huisman, H.J.; Hulsbergen- van de Kaa, C.A.; Basten, JP van; Oort, I van; Witjes, J.A.; Barentsz, J.O.

    2008-01-01

    OBJECTIVES: To test the technique and feasibility of translating tumor suspicious region maps in the prostate, obtained by multimodality, anatomic, and functional 3T magnetic resonance imaging (MRI) data to 32-channel coil, T2-weighted (T2-w), 3T MR images, for directing MR-guided biopsies. Furtherm

  5. Magnetic resonance imaging of congenital heart disease at 0.3 T

    Energy Technology Data Exchange (ETDEWEB)

    Malmgren, N.

    1995-11-01

    The aim of this study was to systematically evaluate the potential of static MR imaging in congenital heart disease. The diagnosis of vascular rings, causing significant respiratory morbidity, previously required invasive methods for diagnosis. In aberrant left pulmonary artery, an uncommon anomaly, MR imaging at 0.3 T provided complete anatomic information. Complex congenital heart malformation is a diagnostic challenge. MR imaging at 0.3 T was efficient in defining most of the components of complex disease. It is especially valuable for imaging of extracardiac structures, which can be difficult to evaluate using echocardiography. MR imaging is an important complement to echocardiography. Improved equipment with sophisticated dynamic applications using high magnetic field strength is now available. The anatomical evaluation of congenital heart anomalies is however still based on static images, and low field magnets will certainly be used even in the future. 141 refs, 11 figs, 8 tabs.

  6. Functional Magnetic Resonance Imaging at 3T as a Clinical Tool in Patients with Intracranial Tumors

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    Westen, D. van; Skagerberg, G.; Olsrud, J.; Fransson, P.; Larsson, E.M. [Univ. Hospital, Lund (Sweden). Depts. of Diagnostic Radiology and Neurosurgery

    2005-10-01

    Purpose: To investigate the potential of functional magnetic resonance imaging (fMRI) at 3T as a clinical tool in the preoperative evaluation of patients with intracranial tumors. High magnetic field strength such as 3T is of benefit for fMRI because signal-to-noise ratio and sensitivity to susceptibility changes are field-strength-dependent. Material and Methods: Twenty patients with tumors close to eloquent sensorimotor or language areas were studied. Motor, sensory, and two language paradigms (word generation, rhyming) were used; their effectiveness was determined as the percentage of patients in whom the functional area of interest was activated. Activation maps were calculated and their quality rated as high, adequate, or insufficient. The influence of fMRI on the neurosurgical decision regarding operability, surgical approach, and extent of the resection, was assessed. Results: Paradigm effectiveness was 90% for motor and 95% for sensory stimulation, and varied from 79% to 95% for word generation and rhyming in combination. Ninety percent of the activation maps held high or adequate quality. fMRI proved useful: in the decision to operate (9 patients), in the surgical approach (13 patients), and in extent of the resection (12 patients). Conclusion: fMRI at 3T is a clinically applicable tool in the work-up of patients with intracranial tumors.

  7. Magnetic resonance spectroscopic imaging 3T and prostate cancer: correlation with transperineal ultrasound guided prostate biopsy.

    Science.gov (United States)

    Castellucci, Roberto; Altieri, Vincenzo Maria; Marchioni, Michele; Castellan, Pietro; Pellegrini, Maurizio; Álvarez-Maestro, Mario; Sánchez-Gómez, Javier; De Francesco, Piergustavo; Ingrosso, Manuela; Tartaro, Armando; Tenaglia, Raffaele Lanfranco

    2015-06-01

    The aim of our study was to correlate the results obtained by 3T Magnetic Resonance Spectroscopic Imaging (MRSI3T) with those obtained by histological examination of samples of the trans-perineal ultrasound-guided prostate biopsy (TPUS-B). 34 patients were enrolled in the study. All patients had a clinical suspicion of cancer due to increased PSA and/or positive digital rectal examination. Patients were subjected to an MRSI 3T examination and subsequently to TPUS-B. Of the 22 (22/34) patients who presented abnormalities MRSI at 3T, 9 had a histological diagnosis of Prostate adenocarcinoma. Of the remaining 13 patients, 6 were found to be histologically positive for Benign Prostatic Hypertrophy and 7 Chronic Interstitial Inflammation or High Grade Prostatic Intraepithelial Neoplasia. 12 (12/34) patients found to have no peripheral alterations in their prostate on 3T MRSI, none were positive for ADK or inflammation on histology. The sensitivity, specificity, positive predictive value and negative predictive value were 100%, 48%, 40% and 100% respectively. In this study, we correlated the values obtained from 3T MRSI with the results of histologically examined prostate biopsies. Our work shows that 72.8% of the voxels in which there was a change in ratio of Cit/(Cho + Cr), corresponded to areas of prostate tissue disease. Of these, 73.2% were positive for ADK and 26.8% for CII or HG PIN. In literature, it is noted that PCa can be distinguished from areas of benign tissue, in the peripheral zone, on the basis of the values of the ratio Cit/(Cho + Cr) (17), although some benign conditions, such as prostatitis or PINHG, can alter these values (18-19). In conclusion, the use of MRSI 3T before performing prostate biopsies may represent a valid aid for the urologist in the diagnosis of PCa, allowing them to avoid unnecessary prostate biopsies that may be negative. Furthermore, it would also be possible to reduce the total number of biopsies, thus decreasing patient exposure

  8. Value of endorectal magnetic resonance imaging at 3T for the local staging of prostate cancer

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    Otto, J.; Thoermer, G.; Seiwerts, M. [Leipzig University Hospital (Germany). Dept. of Diagnostic and Interventional Radiology; and others

    2014-08-15

    Purpose: To assess the accuracy of endorectal 3 T magnetic resonance imaging (MRI) in detecting extracapsular extension (ECE) and seminal vesicle invasion (SVI) of prostate cancer (PCa). Materials and Methods: 38 consecutive patients with biopsy-proven PCa underwent multiparametric endorectal MRI at 3 T prior to prostatectomy. Two readers (A with nine years of experience and B with four) used established criteria for ECE and SVI to diagnose the extent of local disease in six regions (apical, dorsolateral, basal; left and right each) with the highest chance of ECE. The standard of reference was provided by intraoperative frozen section analysis and prostatectomy specimens. Results: Histopathology revealed ECE in 15 of the 222 regions (10 of 37 patients) and SVI in 8 of 74 potential regions (5 of 37 patients). The sensitivity, specificity, and accuracy in detecting ECE for reader A/B were 93%/67%, 92%/95% and 92%/93% per region and 90%/80%, 74%/82% and 78%/81% per patient, respectively. The corresponding values for the detection of SVI were 80%/100%, 96%/99% and 95%/97%, respectively. Conclusion: Endorectal 3 T MRI is a highly reliable noninvasive technique for the local staging of PCa. (orig.)

  9. Successful serial imaging of the mouse cerebral arteries using conventional 3-T magnetic resonance imaging

    Science.gov (United States)

    Makino, Hiroshi; Hokamura, Kazuya; Natsume, Takahiro; Kimura, Tetsuro; Kamio, Yoshinobu; Magata, Yasuhiro; Namba, Hiroki; Katoh, Takasumi; Sato, Shigehito; Hashimoto, Tomoki; Umemura, Kazuo

    2015-01-01

    Serial imaging studies can be useful in characterizing the pathologic and physiologic remodeling of cerebral arteries in various mouse models. We tested the feasibility of using a readily available, conventional 3-T magnetic resonance imaging (MRI) to serially image cerebrovascular remodeling in mice. We utilized a mouse model of intracranial aneurysm as a mouse model of the dynamic, pathologic remodeling of cerebral arteries. Aneurysms were induced by hypertension and a single elastase injection into the cerebrospinal fluid. For the mouse cerebrovascular imaging, we used a conventional 3-T MRI system and a 40-mm saddle coil. We used non-enhanced magnetic resonance angiography (MRA) to detect intracranial aneurysm formation and T2-weighted imaging to detect aneurysmal subarachnoid hemorrhage. A serial MRI was conducted every 2 to 3 days. MRI detection of aneurysm formation and subarachnoid hemorrhage was compared against the postmortem inspection of the brain that was perfused with dye. The imaging times for the MRA and T2-weighted imaging were 3.7±0.5 minutes and 4.8±0.0 minutes, respectively. All aneurysms and subarachnoid hemorrhages were correctly identified by two masked observers on MRI. This MRI-based serial imaging technique was useful in detecting intracranial aneurysm formation and subarachnoid hemorrhage in mice. PMID:25920958

  10. Contributed Review: Nuclear magnetic resonance core analysis at 0.3 T

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    Mitchell, Jonathan, E-mail: JMitchell16@slb.com; Fordham, Edmund J. [Schlumberger Gould Research, High Cross, Madingley Road, Cambridge CB3 0EL (United Kingdom)

    2014-11-15

    Nuclear magnetic resonance (NMR) provides a powerful toolbox for petrophysical characterization of reservoir core plugs and fluids in the laboratory. Previously, there has been considerable focus on low field magnet technology for well log calibration. Now there is renewed interest in the study of reservoir samples using stronger magnets to complement these standard NMR measurements. Here, the capabilities of an imaging magnet with a field strength of 0.3 T (corresponding to 12.9 MHz for proton) are reviewed in the context of reservoir core analysis. Quantitative estimates of porosity (saturation) and pore size distributions are obtained under favorable conditions (e.g., in carbonates), with the added advantage of multidimensional imaging, detection of lower gyromagnetic ratio nuclei, and short probe recovery times that make the system suitable for shale studies. Intermediate field instruments provide quantitative porosity maps of rock plugs that cannot be obtained using high field medical scanners due to the field-dependent susceptibility contrast in the porous medium. Example data are presented that highlight the potential applications of an intermediate field imaging instrument as a complement to low field instruments in core analysis and for materials science studies in general.

  11. Contributed review: nuclear magnetic resonance core analysis at 0.3 T.

    Science.gov (United States)

    Mitchell, Jonathan; Fordham, Edmund J

    2014-11-01

    Nuclear magnetic resonance (NMR) provides a powerful toolbox for petrophysical characterization of reservoir core plugs and fluids in the laboratory. Previously, there has been considerable focus on low field magnet technology for well log calibration. Now there is renewed interest in the study of reservoir samples using stronger magnets to complement these standard NMR measurements. Here, the capabilities of an imaging magnet with a field strength of 0.3 T (corresponding to 12.9 MHz for proton) are reviewed in the context of reservoir core analysis. Quantitative estimates of porosity (saturation) and pore size distributions are obtained under favorable conditions (e.g., in carbonates), with the added advantage of multidimensional imaging, detection of lower gyromagnetic ratio nuclei, and short probe recovery times that make the system suitable for shale studies. Intermediate field instruments provide quantitative porosity maps of rock plugs that cannot be obtained using high field medical scanners due to the field-dependent susceptibility contrast in the porous medium. Example data are presented that highlight the potential applications of an intermediate field imaging instrument as a complement to low field instruments in core analysis and for materials science studies in general.

  12. Study on hippocampal volume with quantitative 3T magnetic resonance imaging in Chinese patients with epilepsy

    Institute of Scientific and Technical Information of China (English)

    GAO Mei-chun; LU Qin-chi; LI Yan-sheng; SHEN Jia-lin

    2012-01-01

    Background It was still rare for the quantitative magnetic resonance imaging (MRI) research of regional changes in hippocampus sclerosis (HS) in Chinese patients with epilepsy.This study aimed to study the hippocampal volumes (HVs)with quantitative MRI measurement in Chinese patients with epilepsy.Methods Forty-six Chinese patients with epilepsy (intractable epilepsy (IE),n=21; non-intractable epilepsy (NIE),n=25)and 25 normal controls were collected between July 2007 and March 2008.All of the subjects underwent a 3T high-resolution MRI with oblique coronal thin sections oriented perpendicular to the hippocampal long axis.Hippocampal structures were assessed by visual detection,and HVs were quantitatively studied with a Picture Archiving and Communication System (PACS).Results Our study suggested that there was no significant difference in gender (P >0.05) while the right hippocampal head volume (HHV),hippocampal body volume (HBV),and the whole hippocampal volume (HCV) were greater than the left one (P <0.05),but no significant difference was found in bilateral hippocampal tail volume (HTV) (P >0.05) in normal controls.That unilateral/diffuse (64%/21%) and bilateral/focal (86%/20%) hippocampal atrophy (HA)were significant in IE and NIE patients,respectively.Anterior hippocampus,especially HHV (26% in IE and 20% in NIE) and HBV (29% in IE and 12% in NIE),had more significant atrophy than the HTV (5% in IE and 0% in NIE) in patients with epilepsy.Conclusion By assessing the volumes of the regional hippocampus with 3T MRI,we could better define the range and distribution of HS,since regional or subtle changes in HVs could be detected earlier with 3T MRI.

  13. Magnetic Levitation of MC3T3 Osteoblast Cells as a Ground-Based Simulation of Microgravity

    Science.gov (United States)

    Kidder, Louis S.; Williams, Philip C.; Xu, Wayne Wenzhong

    2009-01-01

    Diamagnetic samples placed in a strong magnetic field and a magnetic field gradient experience a magnetic force. Stable magnetic levitation occurs when the magnetic force exactly counter balances the gravitational force. Under this condition, a diamagnetic sample is in a simulated microgravity environment. The purpose of this study is to explore if MC3T3-E1 osteoblastic cells can be grown in magnetically simulated hypo-g and hyper-g environments and determine if gene expression is differentially expressed under these conditions. The murine calvarial osteoblastic cell line, MC3T3-E1, grown on Cytodex-3 beads, were subjected to a net gravitational force of 0, 1 and 2 g in a 17 T superconducting magnet for 2 days. Microarray analysis of these cells indicated that gravitational stress leads to up and down regulation of hundreds of genes. The methodology of sustaining long-term magnetic levitation of biological systems are discussed. PMID:20052306

  14. A portable magnetic field of >3 T generated by the flux jump assisted, pulsed field magnetization of bulk superconductors

    Science.gov (United States)

    Zhou, Difan; Ainslie, Mark D.; Shi, Yunhua; Dennis, Anthony R.; Huang, Kaiyuan; Hull, John R.; Cardwell, David A.; Durrell, John H.

    2017-02-01

    A trapped magnetic field of greater than 3 T has been achieved in a single grain GdBa2Cu3O7-δ (GdBaCuO) bulk superconductor of diameter 30 mm by employing pulsed field magnetization. The magnet system is portable and operates at temperatures between 50 K and 60 K. Flux jump behaviour was observed consistently during magnetization when the applied pulsed field, Ba, exceeded a critical value (e.g., 3.78 T at 60 K). A sharp dBa/dt is essential to this phenomenon. This flux jump behaviour enables the magnetic flux to penetrate fully to the centre of the bulk superconductor, resulting in full magnetization of the sample without requiring an applied field as large as that predicted by the Bean model. We show that this flux jump behaviour can occur over a wide range of fields and temperatures, and that it can be exploited in a practical quasi-permanent magnet system.

  15. [Experimental evaluation of the occupational exposure to static magnetic fields on a 3 T magnetic resonance scanner].

    Science.gov (United States)

    Moro, Luca; Alabiso, Francesco; Parisoli, Francesco; Frigerio, Francesco

    2013-01-01

    The recent postponement until 31 October 2013 of the deadline for transposition of the EU Directive 2004/40/EC, concerning the minimum health requirementsfor the exposure of workers to the risks arising from electromagnetic fields between 0 and 300 GHz, keeps on suspending the Italian law which was aimed to implement the EU regulations on the occupational exposure to electromagnetic fields, including those generated by Magnetic Resonance Imaging (MRI) units. Waiting for the revision of the exposure limits proposed by the EU Directive taking into account results from new studies and evolution of knowledge, the time-weighted values of static magnetic field proposed by the Italian Ministry of Health (D.M 02/08/91) still survive as limits for worker's exposure. The comparison between the proposed thresholds and the time required to position patients allows to calculate how long the MRI staff can stay at different values of static magnetic field, i.e. the maximum workload of each worker. In order to evaluate more accurately how many time the members of MRI staff are near the magnet bore and the real value of worker's exposure to the static magnetic field during the handling of patients, a teslameter Metrolab THM1176-PDA was used. Personal exposure measurements on the radiologists and the radiographers who worked on a 3 T GE Healthcare Discovery 750 MR were carried out during the positioning of self-sufficient and collaborative patients. The sensor was worn at the chest level on the side that was nearest to the magnet bore. Results show wide variations occurring between individual working procedures concerning the handling of patients, especially during the initial position phase. The mean values of the time spent by radiographers inside the magnet room (B > 0.5 mT) to place the patient and to take him outside at the end of the exam were respectively 220 and 127 seconds. The mean value of the time spent by radiologists was 162 seconds when they had to insert a peripheral

  16. High-resolution intravascular magnetic resonance quantification of atherosclerotic plaque at 3T

    Directory of Open Access Journals (Sweden)

    Qian Di

    2012-03-01

    Full Text Available Abstract Background The thickness of fibrous caps (FCT of atherosclerotic lesions is a critical factor affecting plaque vulnerability to rupture. This study tests whether 3 Tesla high-resolution intravascular cardiovascular magnetic resonance (CMR employing tiny loopless detectors can identify lesions and accurately measure FCT in human arterial specimens, and whether such an approach is feasible in vivo using animal models. Methods Receive-only 2.2 mm and 0.8 mm diameter intravascular loopless CMR detectors were fabricated for a clinical 3 Tesla MR scanner, and the absolute signal-to-noise ratio determined. The detectors were applied in a two-step protocol comprised of CMR angiography to identify atherosclerotic lesions, followed by high-resolution CMR to characterize FCT, lesion size, and/or vessel wall thickness. The protocol was applied in fresh human iliac and carotid artery specimens in a human-equivalent saline bath. Mean FCT measured by 80 μm intravascular CMR was compared with histology of the same sections. In vivo studies compared aortic wall thickness and plaque size in healthy and hyperlipidemic rabbit models, with post-mortem histology. Results Histology confirmed plaques in human specimens, with calcifications appearing as signal voids. Mean FCT agreed with histological measurements within 13% on average (correlation coefficient, R = 0.98; Bland-Altman analysis, -1.3 ± 68.9 μm. In vivo aortic wall and plaque size measured by 80 μm intravascular CMR agreed with histology. Conclusion Intravascular 3T CMR with loopless detectors can both locate atherosclerotic lesions, and accurately measure FCT at high-resolution in a strategy that appears feasible in vivo. The approach shows promise for quantifying vulnerable plaque for evaluating experimental therapies.

  17. Peripheral Zone Prostate Cancer Localization by Multiparametric Magnetic Resonance at 3 T: Unbiased Cancer Identification by Matching to Histopathology

    NARCIS (Netherlands)

    Selnæs, K.M.; Heerschap, A.; Jensen, L.R.; Tessem, M.-B.; Schweder, G.J.-V.; Goa, P.a., l E.; Viset, T.; Angelsen, A.; Gribbestad, I.S.

    2012-01-01

    OBJECTIVES: The aim of this study was to assess the diagnostic accuracy of peripheral zone prostate cancer localization by multiparametric magnetic resonance (MR) at 3 T using segmental matching of histopathology and MR images to avoid bias by image features in selection of cancer and noncancer regi

  18. Boron nitride nanotubes for boron neutron capture therapy as contrast agents in magnetic resonance imaging at 3 T

    Energy Technology Data Exchange (ETDEWEB)

    Menichetti, L., E-mail: luca.menichetti@ifc.cnr.it [CNR-National Research Council of Italy, Institute of Clinical Physiology, via G. Moruzzi 1, 56124 Pisa (Italy); De Marchi, D. [Fondazione Toscana Gabriele Monasterio per la Ricerca Medica e la Sanita Pubblica, CNR-Regione Toscana, via Trieste 41, 56126 Pisa (Italy); Calucci, L. [CNR-National Research Council of Italy, ICCOM-Institute of OrganoMetallic Chemistry, via G. Moruzzi 1, 56124 Pisa (Italy); Ciofani, G.; Menciassi, A. [Italian Institute of Technology c/o Scuola Superiore Sant' Anna, viale R. Piaggio 34, 56025 Pontedera (Italy); Forte, C. [CNR-National Research Council of Italy, ICCOM-Institute of OrganoMetallic Chemistry, via G. Moruzzi 1, 56124 Pisa (Italy)

    2011-12-15

    The applicability of boron nitride nanotubes (BNNTs) containing Fe paramagnetic impurities as contrast agents in magnetic resonance imaging (MRI) was investigated. The measurement of longitudinal and transverse relaxation times of water protons in homogeneous aqueous dispersions of BNNTs wrapped with poly(L-lysine) at different concentrations allowed longitudinal (r{sub 1}) and transverse (r{sub 2}) relaxivities to be determined at 3 T. The r{sub 2} value was comparable to those of commercial superparamagnetic iron oxide nanoparticles, indicating that Fe-containing BNNTs have the potential to be used as T{sub 2} contrast-enhancement agents in MRI at 3 T.

  19. Orthodontic springs and auxiliary appliances: assessment of magnetic field interactions associated with 1.5 T and 3 T magnetic resonance systems

    Energy Technology Data Exchange (ETDEWEB)

    Kemper, J.; Priest, A.N.; Adam, G. [University Medical Center of Hamburg-Eppendorf, Clinic of Diagnostic and Interventional Radiology, Hamburg (Germany); Schulze, D. [University Hospital of Freiburg, Department of Oral and Maxillofacial Surgery, Freiburg (Germany); Kahl-Nieke, B.; Klocke, A. [University Medical Center of Hamburg-Eppendorf, Department of Orthodontics, Hamburg (Germany)

    2007-02-15

    The objective of this paper is to evaluate magnetic field interactions at 1.5 and 3 T for 20 orthodontic devices used for fixed orthodontic therapy. Twenty springs and auxiliary parts made from varying ferromagnetic alloys were tested for magnetic field interactions in the static magnetic field at 1.5 and 3 T. Magnetic translational force F{sub z} (in millinewtons) was evaluated by determining the deflection angle {beta} [American Society for Testing and Materials (ASTM standard test method)]. Magnetic-field-induced rotational force F{sub rot} was qualitatively determined using a five-point scale. {beta} was found to be >45 in 13(15) devices at 1.5(3) T and translational force F{sub z} exceeded gravitational force F{sub g} on the particular object [F{sub z} 10.17-261.4 mN (10.72-566.4 mN) at 1.5(3) T]. F{sub z} was found to be up to 24.1(47.5)-fold higher than F{sub g} at 1.5(3) T. Corresponding to this, F{sub rot} on the objects was shown to be high at both field strengths ({>=} +3). Three objects (at 1.5 T) and one object (at 3 T) showed deflection angles <45 , but F{sub rot} was found to be {>=} +3 at both field strengths. For the remaining objects, {beta} was below 45 and torque measurements ranged from 0 to +2. Of 20 objects investigated for magnetic field interactions at 1.5(3) T, 13(15) were unsafe in magnetic resonance (MR), based on the ASTM criteria of F{sub z}. The implications of these results for orthodontic patients undergoing MRI are discussed. (orig.)

  20. Feasibility of imaging superficial palmar arch using micro-ultrasound, 7T and 3T magnetic resonance imaging

    Science.gov (United States)

    Pruzan, Alison N; Kaufman, Audrey E; Calcagno, Claudia; Zhou, Yu; Fayad, Zahi A; Mani, Venkatesh

    2017-01-01

    AIM To demonstrate feasibility of vessel wall imaging of the superficial palmar arch using high frequency micro-ultrasound, 7T and 3T magnetic resonance imaging (MRI). METHODS Four subjects (ages 22-50 years) were scanned on a micro-ultrasound system with a 45-MHz transducer (Vevo 2100, VisualSonics). Subjects’ hands were then imaged on a 3T clinical MR scanner (Siemens Biograph MMR) using an 8-channel special purpose phased array carotid coil. Lastly, subjects’ hands were imaged on a 7T clinical MR scanner (Siemens Magnetom 7T Whole Body Scanner) using a custom built 8-channel transmit receive carotid coil. All three imaging modalities were subjectively analyzed for image quality and visualization of the vessel wall. RESULTS Results of this very preliminary study indicated that vessel wall imaging of the superficial palmar arch was feasible with a whole body 7T and 3T MRI in comparison with micro-ultrasound. Subjective analysis of image quality (1-5 scale, 1: poorest, 5: best) from B mode, ultrasound, 3T SPACE MRI and 7T SPACE MRI indicated that the image quality obtained at 7T was superior to both 3T MRI and micro-ultrasound. The 3D SPACE sequence at both 7T and 3T MRI with isotropic voxels allowed for multi-planar reformatting of images and allowed for less operator dependent results as compared to high frequency micro-ultrasound imaging. Although quantitative analysis revealed that there was no significant difference between the three methods, the 7T Tesla trended to have better visibility of the vessel and its wall. CONCLUSION Imaging of smaller arteries at the 7T is feasible for evaluating atherosclerosis burden and may be of clinical relevance in multiple diseases. PMID:28298968

  1. The pilot study of radiology nursing intervention in abdominal 3-T Magnetic Resonance examination

    Directory of Open Access Journals (Sweden)

    Gui-qing Dong

    Full Text Available Abstract OBJECTIVE The aim of this study was to investigate the effect of radiology nursing intervention in abdominal examination at 3-T MRI. METHOD 60 patients with abdominal diseases were divided into two groups randomly: MR nursing intervention group and control group. All the patients underwent abdominal MR examination at 3-T. The MR nursing interventions were performed in nursing intervention group. The outcomes, including one-time success rate, the ratio of diagnosable MR images and the points of image quality, were compared between these two groups. RESULTS The one-time success rates in control group and MR nursing intervention group were 66.67% and 96.67% with significant difference ( χ2 =9.017, P<0.05. The ratios of diagnosable images in the two groups were 76.67% and 96.67% with significant difference (χ2 =5.192, P<0.05. The points of MR image quality in the two groups were 1.87±0.86 and 2.33±0.55, respectively. There was significant difference between these two groups (t=-2.508, P<0.05. CONCLUSION The effective nursing intervention can make the patients cooperation better in abdominal MR examination and improve the image quality significantly.

  2. Dynamic magnetic resonance imaging of swallowing and laryngeal motion using parallel imaging at 3 T.

    Science.gov (United States)

    Breyer, Tobias; Echternach, Matthias; Arndt, Susan; Richter, Bernhard; Speck, Oliver; Schumacher, Martin; Markl, Michael

    2009-01-01

    To evaluate the feasibility of an optimized MRI protocol based on high field imaging at 3 T in combination with accelerated data acquisition by parallel imaging for the analysis of oropharyngeal and laryngeal function. Fast 2D gradient echo (GRE) MRI with different spatial resolutions (1.7x2.7 and 1.1x1.5 mm2) and image update rates (4 and 10 frames per second) was employed to assess pharyngeal movements and visualize swallowing via tracking of an oral contrast bolus (blueberry juice). In a study with 10 normal volunteers, image quality was semi-quantitatively graded by three independent observers with respect to the delineation of anatomical detail and depiction of oropharynx and larynx function. Additionally, the feasibility of the technique for the visualization of pathological pre- and post-surgical oropharynx and larynx function was evaluated in a patient with inspiratory stridor. Image grading demonstrated the feasibility of dynamic MRI for the assessment of normal oropharynx and larynx anatomy and function. Superior image quality (Pswallowing. Results of the volunteer study demonstrated the feasibility of dynamic MRI at 3 T for the visualization of the oropharynx and larynx function during breathing, movements of the tongue and swallowing. Future studies are necessary to evaluate its clinical value compared to existing modalities based on endoscopy or radiographic techniques.

  3. Time-resolved phosphorous magnetization transfer of the human calf muscle at 3 T and 7 T: A feasibility study

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    Valkovič, Ladislav, E-mail: siegfried.trattnig@meduniwien.ac.at [MR Center of Excellence, Department of Radiology, Medical University Vienna, A-1090 Wien, Lazarettgasse 14 (Austria); Institute of Measurement Science, Department of Imaging Methods, Slovak Academy of Sciences, 841 04 Bratislava, Dúbravska cesta 9 (Slovakia); Chmelík, Marek, E-mail: marek.chmelik@meduniwien.ac.at [MR Center of Excellence, Department of Radiology, Medical University Vienna, A-1090 Wien, Lazarettgasse 14 (Austria); Just Kukurova, Ivica, E-mail: ivica.kukurova@meduniwien.ac.at [MR Center of Excellence, Department of Radiology, Medical University Vienna, A-1090 Wien, Lazarettgasse 14 (Austria); Krššák, Martin, E-mail: martin.krssak@meduniwien.ac.at [Department of Internal Medicine III, Medical University Vienna, A-1090 Wien, Lazarettgasse 14 (Austria); Gruber, Stephan, E-mail: stephan@nmr.at [MR Center of Excellence, Department of Radiology, Medical University Vienna, A-1090 Wien, Lazarettgasse 14 (Austria); Frollo, Ivan, E-mail: umerollo@savba.sk [Institute of Measurement Science, Department of Imaging Methods, Slovak Academy of Sciences, 841 04 Bratislava, Dúbravska cesta 9 (Slovakia); Trattnig, Siegfried, E-mail: siegfried.trattnig@meduniwien.ac.at [MR Center of Excellence, Department of Radiology, Medical University Vienna, A-1090 Wien, Lazarettgasse 14 (Austria); Bogner, Wolfgang, E-mail: wolfgang@nmr.at [MR Center of Excellence, Department of Radiology, Medical University Vienna, A-1090 Wien, Lazarettgasse 14 (Austria)

    2013-05-15

    Phosphorous ({sup 31}P) magnetization transfer (MT) experiments enable the non-invasive investigation of human muscle metabolism in various physiological and pathological conditions. The purpose of our study was to investigate the feasibility of time-resolved MT, and to compare the results of MT experiments at 3 T and 7 T. Six healthy volunteers were examined on a 3 T and a 7 T MR scanner using the same setup and identical measurement protocols. In the calf muscle of all volunteers, four separate MT experiments (each ∼10 min duration) were performed in one session. The forward rate constant of the ATP synthesis reaction (k{sub ATP}) and creatine kinase reaction (k{sub CK}), as well as corresponding metabolic fluxes (F{sub ATP}, F{sub CK}), were estimated. A comparison of these exchange parameters, apparent T{sub 1}s, data quality, quantification precision, and reproducibility was performed. The data quality and reproducibility of the same MT experiments at 7 T was significantly higher (i.e., k{sub ATP} 2.7 times higher and k{sub CK} 3.4 times higher) than at 3 T (p < 0.05). The values for k{sub ATP} (p = 0.35) and k{sub CK} (p = 0.09) at both field strengths were indistinguishable. Even a single MT experiment at 7 T provided better data quality than did a 4 times-longer MT experiment at 3 T. The minimal time-resolution to reliably quantify both F{sub ATP} and F{sub CK} at 7 T was ∼6 min. Our results show that MT experiments at 7 T can be at least 4 times faster than 3 T MT experiments and still provide significantly better quantification. This enables time-resolved MT experiments for the observation of slow metabolic changes in the human calf muscle at 7 T.

  4. Occupational exposure levels of static magnetic field during routine MRI examination in 3T MR system.

    Science.gov (United States)

    Yamaguchi-Sekino, Sachiko; Nakai, Toshiharu; Imai, Shinya; Izawa, Shuhei; Okuno, Tsutomu

    2014-01-01

    Occupational exposure to the high static magnetic fields (SMFs) during magnetic resonance imaging (MRI) examinations raises concerns of adverse health effects. In this study, personal exposure monitoring of the magnetic fields during routine examinations in two 3 T MRI systems was carried out. A three-axis Hall magnetometer was attached to a subject's chest during monitoring. Data acquisition started every time the subject entered the scanner room and ended when the subject exited the room. Four radiologic technologists from two different institutes participated in this study. The maximum exposed field ranged from 0 to 1250 mT and the average peak magnetic field (B) was 428 ± 231 mT (mean ± standard deviation (SD): number of samples (N) = 103). Then, the relationship between exposure levels and work duties was analyzed. The MRI examination of the head or neck showed the highest average peak B among four work categories. These results provide information of real exposure levels for 3 T MRI system operators and can also improve the current practical training advice for preventing extra occupational field exposure.

  5. Structural and magnetic properties of TlTF{sub 3} (T=Fe, Co and Ni) by hybrid functional theory

    Energy Technology Data Exchange (ETDEWEB)

    Zeb, Raham [Center for Computational Materials Science, University of Malakand, Chakdara, Dir (Lower) (Pakistan); Department of Physics, University of Malakand, Chakdara, Dir (Lower) (Pakistan); Ali, Zahid, E-mail: zahidf82@gmail.com [Center for Computational Materials Science, University of Malakand, Chakdara, Dir (Lower) (Pakistan); Department of Physics, University of Malakand, Chakdara, Dir (Lower) (Pakistan); Ahmad, Iftikhar; Khan, Imad [Center for Computational Materials Science, University of Malakand, Chakdara, Dir (Lower) (Pakistan); Department of Physics, University of Malakand, Chakdara, Dir (Lower) (Pakistan)

    2015-08-15

    DFT studies are performed to investigate the structural, mechanical and magneto-electronic properties of the TlTF{sub 3} (T=Fe, Co and Ni) perovskites for the first time using GGA, GGA+U and hybrid density functional theory (HF). Our calculations show that HF give better results than GGA and GGA+U and more consistent with the experiments. The comparison of the lattice constants calculated by HF with experiments shows a maximum underestimation less than 0.2%. The chemical bonding between different ions in these compounds is explained on the bases of electronic clouds, which reveals that in TlFeF{sub 3}, Fe has more ionic character with F than the rest. The mechanical properties explain the hardness of these compounds and show that TlFeF{sub 3} is more ductile. Spin-dependent electronic band profiles show that TlFeF{sub 3} and TlCoF{sub 3} are metallic, whereas TlNiF{sub 3} is pseudo direct wide bandgap semiconductor. The stable magnetic phase optimizations and the calculated magnetic susceptibility confirm that TlFeF{sub 3} and TlNiF{sub 3} are ferromagnetic whereas TlCoF{sub 3} is anti-ferromagnetic material. - Highlights: • TlTF{sub 3} (T=Fe, Co and Ni) perovskites are investigated theoretically by hybrid density functional theory. • Mechanical properties explain the hardness of these compounds and show that TlFeF{sub 3} is more ductile. • The bandgap calculations show that TlFeF{sub 3} and TlCoF{sub 3} are metallic, whereas TlNiF{sub 3} is a wide bandgap semiconductor. • Magnetic optimizations and magnetic susceptibility confirm that TlFeF{sub 3} and TlNiF{sub 3} are ferromagnetic whereas TlCoF{sub 3} is anti-ferromagnetic material.

  6. Feasibility of magnetic resonance elastography for the pancreas at 3T.

    Science.gov (United States)

    Itoh, Yohei; Takehara, Yasuo; Kawase, Toshihiro; Terashima, Kenichi; Ohkawa, Yoshihisa; Hirose, Yuko; Koda, Ai; Hyodo, Naoko; Ushio, Takasuke; Hirai, Yuki; Yoshizawa, Nobuko; Yamashita, Shuhei; Nasu, Hatsuko; Ohishi, Naoki; Sakahara, Harumi

    2016-02-01

    1) To assess the usefulness of an elastic belt bracing the upper abdomen for reducing the miscalculated areas of the pancreas on 3.0T magnetic resonance elastography (MRE); 2) to test whether MRE can detect difference of stiffness between normal pancreas and the focal pancreatic diseases. Using an initial eight normal volunteers, miscalculated areas were compared between MRE with the elastic belt and without the belt on 3.0T MRI. Then, using the belt, MRE of the normal pancreas was measured using 14 volunteers and 11 patients with focal pancreatic lesions. The median (95% confidence interval [CI]) percentages of correctly calculated areas were 57.4% (32.9-63.0) with the elastic belt and 35.3% (11.4-60.4) without the belt (P = 0.0078). The stiffness of each pancreatic segment of the normal volunteers (mean ± SE) was 2.37 ± 0.16 kPa for the head, 2.46 ± 0.17 kPa for the body, and 2.58 ± 0.26 kPa for the tail. The stiffness of seven pancreatic cancers was 6.06 ± 0.49 kPa, which was higher than the overall pancreatic stiffness of the normal volunteers (2.47 ± 0.11 kPa, P pancreas and pancreatic lesions. © 2015 Wiley Periodicals, Inc.

  7. Dynamic contrast-enhanced 3-T magnetic resonance imaging: a method for quantifying disease activity in early polyarthritis

    Energy Technology Data Exchange (ETDEWEB)

    Navalho, Marcio [Faculdade de Medicina da Universidade de Lisboa, Rheumatology Research Unit, Instituto de Medicina Molecular, Lisbon (Portugal); Hospital da Luz, Radiology Department, Lisbon (Portugal); Hospital da Luz, Centro de Imagiologia, Lisbon (Portugal); Resende, Catarina [Hospital da Luz, Rheumatology Department, Lisbon (Portugal); Hospital de Santa Maria, Rheumatology Department, Centro Hospitalar de Lisboa Norte, EPE, Lisbon (Portugal); Rodrigues, Ana Maria; Fonseca, Joao Eurico; Canhao, Helena [Faculdade de Medicina da Universidade de Lisboa, Rheumatology Research Unit, Instituto de Medicina Molecular, Lisbon (Portugal); Hospital de Santa Maria, Rheumatology Department, Centro Hospitalar de Lisboa Norte, EPE, Lisbon (Portugal); Gaspar, Augusto [Hospital da Luz, Radiology Department, Lisbon (Portugal); Campos, Jorge [Hospital de Santa Maria, Radiology Department, Centro Hospitalar de Lisboa Norte, EPE, Lisbon (Portugal)

    2012-01-15

    To determine whether measurement of synovial enhancement and thickness quantification parameters with 3.0-Tesla magnetic resonance imaging (3-T MRI) can reliably quantify disease activity in patients with early polyarthritis. Eighteen patients (16 women, 2 men; mean age 46 years) with early polyarthritis with less than 12 months of symptoms were included. MRI examination using 3-T device was performed by a new approach including both wrists and hands simultaneously in the examination field-of-view. MRI scoring of disease activity included quantification of synovial enhancement with simple measurements such as rate of early enhancement (REE; REE{sub 57} = S{sub 57}/S{sub 200}, where S{sub 57} and S{sub 200} are the signal intensities 57 s and 200 s after gadolinium injection) and rate of relative enhancement (RE; RE = S{sub 200} - S{sub 0}). Both wrists and hands were scored according to the Rheumatoid Arthritis MRI Scoring System (RAMRIS) for synovitis. Disease activity was clinically assessed by the 28-joint Disease Activity Score (DAS28). DAS28 score was strongly correlated with RE (r = 0.8331, p < 0.0001), REE (r = 0.8112, p < 0.0001), and RAMRIS score for synovitis (r = 0.7659, p < 0.0002). An REE score above 0.778 accurately identified patients with clinically active disease (sensitivity 92%; specificity 67%; p < 0.05). A statistically significant difference was observed in the RE, REE, and RAMRIS scores for synovitis between patients with active and inactive disease (p < 0.05). Our findings support the use of 3-T dynamic contrast-enhanced MRI for precise quantification of disease activity and for discriminating active disease from inactive disease in early polyarthritis. (orig.)

  8. 3T magnetic resonance neurography of pudendal nerve with cadaveric dissection correlation

    Science.gov (United States)

    Chhabra, Avneesh; McKenna, Courtney A; Wadhwa, Vibhor; Thawait, Gaurav K; Carrino, John A; Lees, Gary P; Dellon, A Lee

    2016-01-01

    AIM To evaluate the pudendal nerve segments that could be identified on magnetic resonance neurography (MRN) before and after surgical marking of different nerve segments. METHODS The hypothesis for this study was that pudendal nerve and its branches would be more easily seen after the surgical nerve marking. Institutional board approval was obtained. One male and one female cadaver pelvis were obtained from the anatomy board and were scanned using 3 Tesla MRI scanner using MR neurography sequences. All possible pudendal nerve branches were identified. The cadavers were then sent to the autopsy lab and were surgically dissected by a peripheral nerve surgeon and an anatomist to identify the pudendal nerve branches. Radiological markers were placed along the course of the pudendal nerve and its branches. The cadavers were then closed and rescanned using the same MRN protocol as the pre-marking scan. The remaining pudendal nerve branches were attempted to be identified using the radiological markers. All scans were read by an experienced musculoskeletal radiologist. RESULTS The pre-marking MR Neurography scans clearly showed the pudendal nerve at its exit from the lumbosacral plexus in the sciatic notch, at the level of the ischial spine and in the Alcock’s Canal in both cadavers. Additionally, the right hemorrhoidal branch could be identified in the male pelvis cadaver. The perineal and distal genital branches could not be identified. On post-marking scans, the markers were used as identifiable structures. The location of the perineal branch, the hemorroidal branch and the dorsal nerve to penis (in male cadaver)/clitoris (in female cadaver) could be seen. However, the visualization of these branches was suboptimal. The contralateral corresponding nerves were poorly seen despite marking on the surgical side. The nerve was best seen on axial T1W and T2W SPAIR images. The proximal segment could be seen well on 3D DW PSIF sequence. T2W SPACE was not very useful in

  9. 3T magnetic resonance neurography of pudendal nerve with cadaveric dissection correlation

    Institute of Scientific and Technical Information of China (English)

    Avneesh Chhabra; Courtney A McKenna; Vibhor Wadhwa; Gaurav K Thawait; John A Carrino; Gary P Lees; A Lee Dellon

    2016-01-01

    AIM: To evaluate the pudendal nerve segments that could be identified on magnetic resonance neurography(MRN) before and after surgical marking of different nerve segments.METHODS: The hypothesis for this study was that pudendal nerve and its branches would be more easily seen after the surgical nerve marking. Institutional board approval was obtained. One male and one female cadaver pelvis were obtained from the anatomy board and were scanned using 3 Tesla MRI scanner using MR neurography sequences. All possible pudendal nerve branches were identified. The cadavers were then sent to the autopsy lab and were surgically dissected by a peripheral nerve surgeon and an anatomist to identify the pudendal nerve branches. Radiological markers were placed along the course of the pudendal nerve and its branches. The cadavers were then closed and rescanned using the same MRN protocol as the premarking scan. The remaining pudendal nerve branches were attempted to be identified using the radiological markers. All scans were read by an experienced musculoskeletal radiologist.RESULTS: The pre-marking MR Neurography scans clearly showed the pudendal nerve at its exit from the lumbosacral plexus in the sciatic notch, at the level of the ischial spine and in the Alcock’s Canal in both cadavers. Additionally, the right hemorrhoidal branch could be identified in the male pelvis cadaver. The perineal and distal genital branches could not be identified. On post-marking scans, the markers were used as identifiable structures. The location of the perineal branch, the hemorroidal branch and the dorsal nerve to penis(in male cadaver)/clitoris(in female cadaver) could be seen. However, the visualization of these branches was suboptimal. The contralateral corresponding nerves were poorly seen despite marking on the surgical side. The nerve was best seen on axial T1W and T2W SPAIR images. The proximal segment could be seen well on 3D DW PSIF sequence. T2W SPACE was not very useful in

  10. Magnetic Resonance Spectroscopy of the Breast at 3T: Pre- and Post-Contrast Evaluation for Breast Lesion Characterization

    Directory of Open Access Journals (Sweden)

    E. Kousi

    2012-01-01

    Full Text Available Purpose. To determine whether in vivo proton magnetic resonance spectroscopy at 3T can provide accurate breast lesion characterization, and to determine the effect of gadolinium on the resonance of tCho. Methods. Twenty-four positive-mammogram patients were examined on a 3T MR scanner. 1H-MRS was performed before and after gadolinium administration. tCho peak was qualitatively evaluated before and after contrast injection. Results. Fourteen out of 27 lesions proved to be malignant after histopathological diagnosis. Using 1H-MRS, before contrast injection, 6/14 confirmed malignancies and 11/13 benign lesions were correctly classified; while, after contrast injection, 11/14 confirmed malignancies and 12/13 benign processes were correctly classified. Post gadolinium 1H-MRS proved useful in picking up tCho signal, improving the overall accuracy, sensitivity, and specificity by 35%, 83%, and 9%, respectively. Conclusion. 1H-MRS overall accuracy, sensitivity, and specificity in detecting breast lesion’s malignancy were increased after gadolinium administration. It is prudent to perform 1H-MRS before contrast injection in large breast lesions to avoid choline underestimation. In cases of small or non-mass lesions, it is recommended to perform 1H-MRS after contrast injection for better voxel prescription to enable a reliable preoperative diagnosis.

  11. Clinical safety of cardiac magnetic resonance imaging at 3 T early after stent placement for acute myocardial infarction

    Energy Technology Data Exchange (ETDEWEB)

    Jehl, Jerome; Comte, Alexandre; Aubry, Sebastien; Kastler, Bruno [Hopital Jean Minjoz, Service de Radiologie A and C, Centre Hospitalier Universitaire de Besancon, Besancon (France); Meneveau, Nicolas; Schiele, Francois [Hopital Jean Minjoz, Service de Cardiologie, Centre Hospitalier Universitaire de Besancon, Besancon (France)

    2009-12-15

    The purpose of this study was to prospectively evaluate the safety of cardiac magnetic resonance (CMR) imaging at 3 T performed early (less than 14 days) after bare metal or drug-eluting coronary stent implantation in patients with acute myocardial infarction (AMI). Seventy-two consecutive patients with AMI treated by percutaneous revascularisation with a stent underwent CMR examination with a median delay of 6 days. Patients were followed-up for major adverse cardiac events, during hospitalisation and at 6 months. After CMR imaging, no acute stent thrombosis, death or repeated AMI were recorded at 6-month follow-up. Two symptomatic in-stent restenoses and two silent in-stent restenoses were recorded, at a mean delay of 106 days. In our population, we found a target revascularisation rate of 5.6%. This is consistent with the 6-month event rates after coronary artery stent (CAS) placement for AMI, evaluated by several studies. This preliminary clinical study supports the safety of 3-T CMR imaging performed early after coronary stent placement. (orig.)

  12. Prostate cancer recurrence after radical prostatectomy: the role of 3-T diffusion imaging in multi-parametric magnetic resonance imaging.

    Science.gov (United States)

    Panebianco, Valeria; Barchetti, Flavio; Sciarra, Alessandro; Musio, Daniela; Forte, Valerio; Gentile, Vincenzo; Tombolini, Vincenzo; Catalano, Carlo

    2013-06-01

    To validate the role of 3-T diffusion-weighted imaging (DWI) in the detection of local prostate cancer recurrence after radical prostatectomy (RP). T2-weighted imaging, DWI and dynamic contrast-enhanced MRI (DCE-MRI) were performed with a 3-T magnet in 262 patients after RP. Twenty out of 262 patients evaluated were excluded. MRI results were validated by prostate-specific antigen (PSA) reduction after external beam radiotherapy in group A (126 patients, local recurrence size range 4-8 mm) and by transrectal ultrasound biopsy in group B (116 patients, local recurrence size range 9-15 mm). In group A combined T2-weighted and DCE-MRI (T2+DCE) shows 98 % sensitivity, 94 % specificity and 93 % accuracy in identifying local recurrence; combined T2-weighted and DWI with a b value of 3,000 s/mm(2) (T2+DW3) displays 97 % sensitivity, 95 % specificity and 92 % accuracy, while with a b value of 1,000 s/mm(2) (T2+DW1) affords 93 % sensitivity, 89 % specificity and 88 % accuracy. In group B T2+DCE shows 100 % sensitivity, 97 % specificity and 91 % accuracy in detecting local cancer recurrence; T2+DW3 displays 98 % sensitivity, 96 % specificity and 89 % accuracy; T2+DW1 has 94 % sensitivity, 92 % specificity and 86 % accuracy. DCE-MRI is the most reliable technique in detecting local prostate cancer recurrence after RP, though DWI can be proposed as a reliable alternative. • Diffusion-weighted magnetic resonance imaging (DWI-MRI) is being increasingly used in oncology. • PSA analysis does not distinguish prostate cancer recurrence from distant metastasis. • DWI-MR can diagnose local prostate cancer recurrence after radical prostatectomy. • DWI-MR is almost comparable to DCE-MRI in detecting local recurrence.

  13. 3T3 cell motility and morphology before, during, and after exposure to extremely-low-frequency magnetic fields

    Energy Technology Data Exchange (ETDEWEB)

    Spadinger, I.; Palcic, B. [British Columbia Cancer Research Centre, Vancouver, British Columbia (Canada). Cancer Imaging; Agnew, D. [Ontario Hydro, Whitby, Ontario (Canada). Health and Safety Div.

    1995-08-01

    Automated image cytometry techniques were used to measure motility and morphology in 3T3 fibro-blasts exposed to extremely-low-frequency (ELF) magnetic fields. Cell motility and morphology were measured as a function of time before, during, and after 3--4 hour exposures to vertically oriented, 100 {mu}T{sub RMS} sinusoidal magnetic fields at various frequencies in the 10--63 Hz range. Sham exposures were also carried out. No static DC fields were applied, but the geomagnetic field was almost vertical and, therefore, had a large component (28.3 {mu}T) parallel to the applied AC field. The morphology and motile behavior of the cells were characterized by mathematically defined descriptors, which were calculated and averaged for the exposure period as well as for control periods that preceded and followed the exposure period. Each experiment involved the tracking of 100 cells that were subjected to one of the test frequencies (unless a sham exposure was being conducted). Statistical analysis of the results showed that even small changes of 10--20% could be significant at the P < .05 level. Changes on this order were measured in a significant proportion of the experiments. However, because such results were seen for both the sham-exposed and the ELF-exposed cells, and because the range of values that was obtained for the sham exposures was the same as that obtained for the ELF exposures, the authors concluded that there was no evidence to show that any of the measured changes were attributable to the applied ELF magnetic field.

  14. Preoperative 3T high field blood oxygen level dependent functional magnetic resonance imaging for glioma involving sensory cortical areas

    Institute of Scientific and Technical Information of China (English)

    LI Shao-wu; WANG Jiang-fei; JIANG Tao; LI Shou-wei; ZHANG Wen-bo; LI Zi-xiao; ZHANG Zhong; DAI Jian-ping; WANG Zhong-cheng

    2010-01-01

    Background Localization of sensory cortical areas during the operation is essential to preserve the sensory function.Intraoperative direct electrostimulation under awake anesthesia is the golden standard but time-consuming. We applied 3T high field blood oxygen level-dependent (BOLD) functional magnetic resonance imaging (fMRI) to identify the relationship between glioma and cortical sensory areas preoperatively and to guide intraoperative direct electrostimulation for quick and precise localization.Methods Five glioma patients with sensory cortex involvement by or next to the lesion had preoperative BOLD fMRI to determine the spatial relationship of cortical sensory areas to the tumours. Bilateral hand opposite movement was performed by these patients for fMRI. Precentral and postcentral gyri were identified by electrical stimulation during the operation. Karnofsky Performance Status scores of the patients' pre- and postoperative and the role of BOLD fMRI were evaluated.Results The cortical sensory areas were all activated in five glioma patients involving postcentral gyrus areas by BOLDf MRI with bilateral hand opposite movement. The detected activation areas corresponded with the results from cortical electrical stimulation.Conclusions The relationship between cortical sensory areas and tumour can be accurately shown by BOLD fMRI before operation. And the information used to make the tumour resection could obtain good clinical results.

  15. Metabolic atrophy and 3-T 1H-magnetic resonance spectroscopy correlation after radiation therapy for prostate cancer.

    Science.gov (United States)

    Panebianco, Valeria; Barchetti, Flavio; Musio, Daniela; Forte, Valerio; Pace, Alberto; De Felice, Francesca; Barchetti, Giovanni; Tombolini, Vincenzo; Catalano, Carlo

    2014-12-01

    To correlate 3-T magnetic resonance spectroscopic imaging (MRSI) with prostate-specific antigen (PSA) levels in patients with prostate cancer treated with external beam radiation therapy to assess the potential advantages of MRSI. A total of 50 patients (age range 65-83 years) underwent PSA and MRSI surveillance before and at 3, 6, 12, 18 and 24 months after radiotherapy. Of the 50 patients examined, 13 patients completely responded to therapy showing metabolic atrophy (MA), defined as a choline-plus-creatine/citrate (CC/C) ratio 0.8). Three of those patients with recurrence had a biochemical relapse at 18 months and the other two at 24 months. Two of the 50 patients did not respond to the treatment, showing persistent disease from the 3rd month (CC/C ratio >0.8); one patient had biochemical relapse at 6 and the other at 12 months. MRSI was shown to have a greater potential than PSA level in monitoring patients after radiotherapy, because it anticipates PSA nadir, and biochemical relapse in particular. © 2013 The Authors. BJU International © 2013 BJU International.

  16. Dynamic contrast-enhanced magnetic resonance angiography for the localization of spinal dural arteriovenous fistulas at 3T.

    Science.gov (United States)

    Zhou, G; Li, M H; Lu, C; Yin, Y L; Zhu, Y Q; Wei, X E; Lu, H T; Zheng, Q Q; Gao, W W

    2017-02-01

    This study was undertaken to evaluate the accuracy of dynamic contrast-enhanced magnetic resonance angiography (DCE-MRA) in the precise location and demonstration of fistulous points in spinal dural arteriovenous fistulas (SDAVFs). Fifteen patients (14 men, 1 woman; age range: 40-78 years; mean: 55.5 years) harboring SDAVF who underwent preoperative DCE-MRA and spinal digital subtraction angiography (DSA) between January 2012 and January 2015 were evaluated retrospectively. Two reviewers independently evaluated the level and side of the arteriovenous fistula and feeding artery on 3T DCE-MRA and DSA images. The accuracy of DCE-MRA was assessed by comparing its findings with those from DSA and surgery in each case. All 15 patients underwent DCE-MRA and DSA. DSA was unsuccessful in two patients due to technical difficulties. All cases were explored surgically, guided by the DCE-MRA. Surgery confirmed that 14 AVF sites were located in the thoracic spine, 5 in the lumbar spine, and 1 in the cervical spine. The origin of the fistulas and feeding arteries was accurately shown by DCE-MRA in 11 of the 15 patients. DCE-MRA also detected dilated perimedullary veins in all 15 patients. Overall, DCE-MRA facilitated DSA catheterization in 10 cases. In six patients, the artery of Adamkiewicz could be observed. In 15 out of 20 fistulas (75%), both readers agreed on the location on DCE-MRA images, and the κ coefficient of the interobserver agreement was 0.67 (95% confidence interval [CI], 0.16-0.87). In 13 of 16 shunts (75%), the DCE-MRA consensus findings and DSA findings coincided. The intermodality agreement was 0.77 (95% CI: 0.35-0.92). Our DCE-MRA studies benefited from the use of a high-field 3T MR imaging unit and reliably detected and localized the SDAVF and feeding arteries. As experience with this technique grows, it may be possible to replace DSA with DCE-MRA if surgery is the planned treatment. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  17. Predictive value of negative 3T multiparametric magnetic resonance imaging of the prostate on 12-core biopsy results.

    Science.gov (United States)

    Wysock, James S; Mendhiratta, Neil; Zattoni, Fabio; Meng, Xiaosong; Bjurlin, Marc; Huang, William C; Lepor, Herbert; Rosenkrantz, Andrew B; Taneja, Samir S

    2016-10-01

    To evaluate the cancer detection rates for men undergoing 12-core systematic prostate biopsy with negative prebiopsy multiparametric magnetic resonance imaging (mpMRI) results. Clinical data from consecutive men undergoing prostate biopsy who had undergone prebiopsy 3T mpMRI from December 2011 to August 2014 were reviewed from an institutional review board-approved prospective database. Men with negative prebiospy mpMRI results (negMRI) before biopsy were identified for the present analysis. Clinical features, cancer detection rates and negative predictive values were summarized. Seventy five men with negMRI underwent systematic 12-core biopsy during the study period. In the entire cohort, men with no previous biopsy, men with previously negative biopsy and men enrolled in active surveillance protocols, the overall cancer detection rates were 18.7, 13.8, 8.0 and 38.1%, respectively, and the detection rates for Gleason score (GS) ≥7 cancer were 1.3, 0, 4.0 and 0%, respectively. The NPVs for all cancers were 81.3, 86.2, 92.0, and 61.9, and for GS ≥7 cancer they were 98.7, 100, 96.0 and 100%, respectively. A negative prebiopsy mpMRI confers an overall NPV of 82% on 12-core biopsy for all cancer and 98% for GS ≥7 cancer. Based on biopsy indication, these findings assist in prebiopsy risk stratification for detection of high-risk disease and may provide guidance in the decision to pursue biopsy. © 2016 The Authors BJU International © 2016 BJU International Published by John Wiley & Sons Ltd.

  18. VITRA miniatuurid Interas

    Index Scriptorium Estoniae

    2007-01-01

    Intera sisustussalongis Pärnu mnt. 160D 31. X-17. XI avatud näitusest "100 Miniatures from the Vitra Design Museum". Eksponeeritud miniatuursed koopiad klassikalistest toolidest, mis on loodud aastast 1850 kuni tänapäevani

  19. Magnetic properties of mixed valence La2/3Sr1/3Mn1−TO3 (T = Fe,Cr) manganites obtained by Pechini method

    Indian Academy of Sciences (India)

    I Betancourt; A Morales-Hernández

    2013-05-01

    Polycrystalline manganites of composition La2/3Sr1/3Mn1−TO3 (T = Cr3+ or Fe3+, x = 0.0-0.10) were obtained by the Pechini method. Their magnetic properties exhibited a marked dependence on Fe/Cr content, with significant reduction of the magnetic moment per formula unit and their Curie temperatures. In addition, the magnetocaloric effect, determined by isothermal magnetization measurements, displayed a decreasing tendency with increasing Fe/Cr concentration. Results were interpreted on the basis of a deleterious effect on the double exchange interaction provoked by the presence of Fe3+ and Cr3+ ions within the crystal structure.

  20. The effectiveness of 3T time-of-flight magnetic resonance angiography for follow-up evaluations after the stent-assisted coil embolization of cerebral aneurysms.

    Science.gov (United States)

    Cho, Won-Sang; Kim, Sam Soo; Lee, Seung Jin; Kim, Sung Hun

    2014-06-01

    Artifacts introduced by stents limit the value of magnetic resonance (MR) imaging as a follow-up modality after the stent-assisted coil embolization of cerebral aneurysms. To investigate the usefulness of 3 Tesla (3T) time-of-flight (TOF) MR angiography (MRA) for the follow-up evaluation. Twenty-two aneurysms of 20 patients treated with stent-assisted coil embolization were followed up with 3T TOF MRA and digital subtraction angiography (DSA) with three-dimensional rotational angiography (3DRA). The status of coiled aneurysms was compared with 3T TOF MRA and DSA with 3DRA in terms of complete occlusion, residual neck, and residual aneurysm. TOF MRA at 3T was performed 1 day before DSA with 3DRA, with a mean follow-up period of 20.1 ± 10.8 months. Twenty (90.9%) of 22 cases were concordant between the two modalities. The degree of agreement and correlation between them were high (κ=0.771, Pcoil embolization of cerebral aneurysms. Further study with a larger patient sample is needed to confirm the effectiveness of 3T TOF MRA. © The Foundation Acta Radiologica 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  1. Osteogenic differentiation of MC3T3-E1 cells on poly(L-lactide)/Fe{sub 3}O{sub 4} nanofibers with static magnetic field exposure

    Energy Technology Data Exchange (ETDEWEB)

    Cai, Qing [State Key Laboratory of Organic–inorganic Composites, Beijing University of Chemical Technology, Beijing 100029 (China); Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029 (China); Shi, Yuzhou; Shan, Dingying; Jia, Wenkai; Duan, Shun [Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029 (China); Deng, Xuliang [Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081 (China); Yang, Xiaoping, E-mail: yangxp@mail.buct.edu.cn [State Key Laboratory of Organic–inorganic Composites, Beijing University of Chemical Technology, Beijing 100029 (China); Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029 (China)

    2015-10-01

    Proliferation and differentiation of bone-related cells are modulated by many factors such as scaffold design, growth factor, dynamic culture system, and physical simulation. Nanofibrous structure and moderate-intensity (1 mT–1 T) static magnetic field (SMF) have been identified as capable of stimulating proliferation and differentiation of osteoblasts. Herein, magnetic nanofibers were prepared by electrospinning mixture solutions of poly(L-lactide) (PLLA) and ferromagnetic Fe{sub 3}O{sub 4} nanoparticles (NPs). The PLLA/Fe{sub 3}O{sub 4} composite nanofibers demonstrated homogeneous dispersion of Fe{sub 3}O{sub 4} NPs, and their magnetism depended on the contents of Fe{sub 3}O{sub 4} NPs. SMF of 100 mT was applied in the culture of MC3T3-E1 osteoblasts on pure PLLA and PLLA/Fe{sub 3}O{sub 4} composite nanofibers for the purpose of studying the effect of SMF on osteogenic differentiation of osteoblastic cells on magnetic nanofibrous scaffolds. On non-magnetic PLLA nanofibers, the application of external SMF could enhance the proliferation and osteogenic differentiation of MC3T3-E1 cells. In comparison with pure PLLA nanofibers, the incorporation of Fe{sub 3}O{sub 4} NPs could also promote the proliferation and osteogenic differentiation of MC3T3-E1 cells in the absence or presence of external SMF. The marriage of magnetic nanofibers and external SMF was found most effective in accelerating every aspect of biological behaviors of MC3T3-E1 osteoblasts. The findings demonstrated that the magnetic feature of substrate and microenvironment were applicable ways in regulating osteogenesis in bone tissue engineering. - Highlights: • Magnetic nanofibers containing well-dispersed Fe{sub 3}O{sub 4} nanoparticles were produced. • Static magnetic field (SMF) was applied to perform the culture of osteoblasts. • Osteogenic differentiation was enhanced on magnetic substrate with exposure to SMF.

  2. Design, construction and 13 K conduction-cooled operation of a 3 T 100 mm stainless steel cladding all-REBCO magnet

    Science.gov (United States)

    Jang, Jae Young; Yoon, Sangwon; Hahn, Seungyong; Hwang, Young Jin; Kim, Jaemin; Shin, Kang Hwan; Cheon, Kyekun; Kim, Kwanglok; In, Sehwan; Hong, Yong-Ju; Yeom, Hankil; Lee, Hunju; Moon, Seung-Hyun; Lee, SangGap

    2017-10-01

    A conduction-cooled 3 T 100 mm winding bore multi-width and no-insulation (NI) all-REBCO magnet was designed, constructed and tested at 13 K. The magnet consists of a stack of double pancake (DP) coils wound with, for the first time, REBCO tapes having a 1 μm thick layer of stainless steel, named ‘metallic cladding’, that surrounds the tapes in a hermetic way to substantially reduce the NI charging delay. After construction, the magnet was cooled down to the target operating temperature of 13 K using a two-stage pulse-tube cryo-cooler. During charging–discharging tests up to 200 A, magnetic center field, voltage of each DP coil, power supply current, and magnet temperature were monitored. The charging time constant of the magnet was measured to be about 10.1 s, 13 times smaller than that of its NI counterpart. The magnet experienced, due to an unexpected power supply trip, a sudden discharge at a peak coil current density of 353 A mm2, yet it survived without any degradation. The results demonstrated strong potential of the metallic cladding NI-REBCO magnet for significant charging-delay reduction and self-protecting operation.

  3. Diagnostic accuracy of 3-T magnetic resonance imaging with 3D T1 VIBE versus computer tomography in pars stress fracture of the lumbar spine

    Energy Technology Data Exchange (ETDEWEB)

    Ang, E.C.; Robertson, A.F.; Malara, F.A.; O' Shea, T.; Roebert, J.K.; Rotstein, A.H. [Victoria House Medical Imaging, Prahran, Victoria (Australia); Schneider, M.E. [Monash University, Monash Biomedicine Discovery Institute, Department of Medical Imaging and Radiation Sciences, Faculty of Medicine, Nursing and Health Sciences, Clayton, Victoria (Australia)

    2016-11-15

    To compare the diagnostic accuracy of 3-T magnetic resonance imaging (MRI) with thin-slice 3D T1 VIBE sequence to 128-slice computer tomography (CT) in pars stress fractures of the lumbar spine. 3-T MRI and CT of 24 patients involving 70 pars interarticularis were retrospectively reviewed by four blinded radiologists. The fracture morphology (complete, incomplete, or normal) was assessed on MRI and CT at different time points. Pars interarticularis bone marrow edema (present or absent) was also evaluated on MRI. In total, 14 complete fractures, 31 incomplete fractures and 25 normal pars were detected by CT. Bone marrow edema was seen in seven of the complete and 25 of the incomplete fractures. The overall sensitivity, specificity and accuracy of MRI in detecting fractures (complete and incomplete) were 97.7, 92.3, and 95.7 %, respectively. MRI was 100 % accurate in detecting complete fractures. For incomplete fractures, the sensitivity, specificity, and accuracy of MRI were 96.7, 92.0, and 94.6 %, respectively. 3-T MRI with thin-slice 3D T1 VIBE is 100 % accurate in diagnosing complete pars fractures and has excellent diagnostic ability in the detection and characterization of incomplete pars stress fractures compared to CT. MRI has the added advantages of detecting bone marrow edema and does not employ ionizing radiation. (orig.)

  4. Detection of atherosclerotic plaque progression in the abdominal aorta of rabbits with 3T magnetic resonance imaging

    Institute of Scientific and Technical Information of China (English)

    MA Xiao-hai; ZHAO Lei; ZHAO Quan-ming; FENG Ting-ting; SHANG Jian-feng; ZHANG Zhao-qi

    2012-01-01

    Background With features of high tissue contrast,MRI can be used for the qualitative and quantitative evaluation of atherosclerosis plaques.In this study we investigated the development of atherosclerosis plaque with high resolution 3T MRI in a rabbit model and compared the findings with the histopathological results.Method Twenty male New Zealand white rabbits were randomly allocated into an experimental group (n=16) and a control group (n=4).Atherosclerotic lesions were induced in the abdominal aorta by balloon injury and cholesterol feeding.Multiple sequences MRI examination (ToF,T1WI,T2WI,and CE T1WI) were performed at the 2nd,3rd,and 4th months after aortic denudation.Vessel wall thickness,total vessel area,lumen area,and vessel wall area were recorded.Plaque components were analyzed using histological results as a standard reference.Results Seventeen rabbits (14 in the experimental group and 3 in the control group) received all three MR examinations.Gradually,from 2 months to 4 months,vessel wall thickness and area in the experimental group increased significantly compared with the control group (P <0.01).In the lumen area progressive stenosis was not found,even a slight dilation had developed in the experimental group.Lipid,fibretic and calcified plaques can be differentiated by MR image.According to histological results,MRI had good performance in detection of lipid plaque.Conclusion MRI can be used to monitor progression of atherosclerosis and differentiate plaque components.

  5. Repair of distal biceps brachii tendon assessed with 3-T magnetic resonance imaging and correlation with functional outcome

    Energy Technology Data Exchange (ETDEWEB)

    Alemann, Guillaume; Dietsch, Emmanuel [University Hospital of Besancon, Department of Musculoskeletal Imaging, Besancon (France); Gallinet, David; Obert, Laurent [University Hospital of Besancon, Department of Orthopedic Surgery, Besancon (France); Kastler, Bruno; Aubry, Sebastien [University Hospital of Besancon, Department of Musculoskeletal Imaging, Besancon (France); Franche Comte University, I4S Laboratory-EA 4268-IFR 133, Besancon (France)

    2015-05-01

    Objectives were to study the MRI appearance of the repaired distal biceps tendon (DBT), anatomically reinserted, and to search for a correlation between tendon measurements and functional results. Twenty-five patients (mean age, 49 ± 4.9 years old) who benefited from 3-T MRI follow-up of the elbow after surgical reinsertion of the DBT were retrospectively included and compared to a control group (n = 25; mean age, 48 ± 10 years old). MRI was performed during the month of clinical follow-up and on average 22 months after surgery. Delayed complications (secondary avulsion, new rupture), intratendinous osteoma, tendinous signal on T1-weighted (T1{sub w}) and fat-suppressed proton density-weighted (FS-PD{sub w}) images as well as DBT measurements were recorded. The maximum isometric elbow flexion strength (MEFS) and range of motion of the elbow were assessed. Repaired DBT demonstrated a heterogeneous but normally fibrillar structure. Its low T1{sub w} signal was less pronounced than that of normal tendons, and the FS-PD{sub W} image signal was similar to that of T1{sub w} images. MRI detected seven osteomas (Se = 53 % vs. plain radiography), one textiloma and one secondary avulsion. Repaired DBT measurements were significantly correlated with MEFS (dominant arm R2: 0.38; nondominant arm R2: 0.54); this correlation involved the insertion surface (Δ = -75.7 mm{sup 2}, p = 0.046), transverse diameter (Δ = -2.6 mm, p = 0.018), anteroposterior diameter at the level of the radial head (Δ = -3.9 mm, p = 0.001) and DBT cross-sectional area (Δ = -50.2 mm{sup 2}, p = 0.003). The quality of functional outcome after anatomical elbow rehabilitation of DBT correlates with the extent of tendinous hypertrophy during the healing process. (orig.)

  6. Improved reliability in skeletal age assessment using a pediatric hand MR scanner with a 0.3T permanent magnet.

    Science.gov (United States)

    Terada, Yasuhiko; Kono, Saki; Uchiumi, Tomomi; Kose, Katsumi; Miyagi, Ryo; Yamabe, Eiko; Fujinaga, Yasunari; Yoshioka, Hiroshi

    2014-01-01

    The purpose of this study was to improve the reliability and validity of skeletal age assessment using an open and compact pediatric hand magnetic resonance (MR) imaging scanner. We used such a scanner with 0.3-tesla permanent magnet to image the left hands of 88 healthy children (aged 3.4 to 15.7 years, mean 8.8 years), and 3 raters (2 orthopedic specialists and a radiologist) assessed skeletal age using those images. We measured the strength of agreement in ratings by values of weighted Cohen's κ and the proportion of cases excluded from rating because of motion artifact and inappropriate positioning. We compared the current results with those of a previous study in which 93 healthy children (aged 4.1 to 16.4 years, mean 9.7 years) were examined with an adult hand scanner. The κ values between raters exceeded 0.80, which indicates almost perfect agreement, and most were higher than those of the previous study. The proportion of cases excluded from rating because of motion artifact or inappropriate positioning was also reduced. The results indicate that use of the compact pediatric hand scanner improved the reliability and validity of skeletal age assessments.

  7. Thyroid Incidentaloma Detected by Time-Resolved Magnetic Resonance Angiography at 3T: Prevalence and Clinical Significance

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Nami; Moon, Won Jin; Kim, Hahn Young; Roh, Hong Gee; Choi, Jin Woo [Konkuk University Medical Center, Seoul (Korea, Republic of)

    2012-06-15

    To determine the prevalence of thyroid incidentalomas detected by time-resolved magnetic resonance angiography (TRMRA) and to evaluate their clinical significance by using an ultrasonographic (US) and cytologic correlation. We retrospectively reviewed 2010 consecutive TRMRA examinations performed at our institution between August 2006 and April 2010. The TRMRA findings of thyroid incidentalomas were analyzed according to location, size, as well as vascularity, and were correlated with the US findings and cytologic results. Each nodule was classified as suspiciously malignant, indeterminate or probably benign according to the US criteria recommended by the Korean Society of Thyroid Radiology. A total of 102 incidentalomas were detected in 90 of 2010 patients (5%). TRMRA showed homogenous hypervascularity in 48 (47%), inhomogeneous hypervascularity in 46 (45%), and hypovascularity in 8 (8%) thyroid nodules. At follow-up study, out of 26 patients with 30 incidentalomas on TRMRA, 27 nodules were identified on US. Of the 27 nodules, 24 (89%) nodule were classified as indeterminate, two (7%) as probably benign, and one (4%) as suspiciously malignant. Among the 16 nodules with available cytopathologic results, 14 (82%) were benign, one (6%) was indeterminate, and one (6%) was malignant. Thyroid incidentalomas are found in 5% of TRMRA examinations. Although their presence does not necessarily indicate malignancy, nonspecific findings of detected incidentalomas on TRMRA require further evaluation by US.

  8. Three-dimensional regional strain analysis in porcine myocardial infarction: a 3T magnetic resonance tagging study

    Directory of Open Access Journals (Sweden)

    Soleimanifard Sahar

    2012-12-01

    Full Text Available Abstract Background Previous studies of mechanical strain anomalies in myocardial infarction (MI have been largely limited to analysis of one-dimensional (1D and two-dimensional (2D strain parameters. Advances in cardiovascular magnetic resonance (CMR methods now permit a complete three-dimensional (3D interrogation of myocardial regional strain. The aim of this study was to investigate the incremental value of CMR-based 3D strain and to test the hypothesis that 3D strain is superior to 1D or 2D strain analysis in the assessment of viability using a porcine model of infarction. Methods Infarction was induced surgically in 20 farm pigs. Cine, late gadolinium enhancement, and CMR tagging images were acquired at 11 days before (baseline, and 11 days (early and 1 month (late after induction of infarct. Harmonic phase analysis was performed to measure circumferential, longitudinal, and radial strains in myocardial segments, which were defined based on the transmurality of delayed enhancement. Univariate, bivariate, and multivariate logistic regression models of strain parameters were created and analyzed to compare the overall diagnostic accuracy of 3D strain analysis with 1D and 2D analyses in identifying the infarct and its adjacent regions from healthy myocardium. Results 3D strain differed significantly in infarct, adjacent, and remote segments (p  Conclusions Cumulative 3D strain information accurately identifies infarcts and their neighboring regions from healthy myocardium. The 3D interrogation of myocardial contractility provides incremental diagnostic accuracy in delineating the dysfunctional and nonviable myocardium in comparison with 1D or 2D quantification of strain. The infarct neighboring regions are the major beneficiaries of the 3D assessment of regional strain.

  9. 3D volumetry comparison using 3T magnetic resonance imaging between normal and adenoma-containing pituitary glands

    Directory of Open Access Journals (Sweden)

    Ernesto Roldan-Valadez

    2011-01-01

    Full Text Available Background: Computed-assisted three-dimensional data (3D allows for an accurate evaluation of volumes compared with traditional measurements. Aims: An in vitro method comparison between geometric volume and 3D volumetry to obtain reference data for pituitary volumes in normal pituitary glands (PGs and PGs containing adenomas. Design: Prospective, transverse, analytical study. Materials and Methods: Forty-eight subjects underwent brain magnetic resonance imaging (MRI with 3D sequencing for computer-aided volumetry. PG phantom volumes by both methods were compared. Using the best volumetric method, volumes of normal PGs and PGs with adenoma were compared. Statistical analysis used the Bland-Altman method, t-statistics, effect size and linear regression analysis. Results: Method comparison between 3D volumetry and geometric volume revealed a lower bias and precision for 3D volumetry. A total of 27 patients exhibited normal PGs (mean age, 42.07 ± 16.17 years, although length, height, width, geometric volume and 3D volumetry were greater in women than in men. A total of 21 patients exhibited adenomas (mean age 39.62 ± 10.79 years, and length, height, width, geometric volume and 3D volumetry were greater in men than in women, with significant volumetric differences. Age did not influence pituitary volumes on linear regression analysis. Conclusions: Results from the present study showed that 3D volumetry was more accurate than the geometric method. In addition, the upper normal limits of PGs overlapped with lower volume limits during early stage microadenomas.

  10. Magnetic resonance elastography in a rabbit model of liver fibrosis: a 3-T longitudinal validation for clinical translation.

    Science.gov (United States)

    Zou, Liqiu; Jiang, Jinzhao; Zhong, Wenxin; Wang, Chunrong; Xing, Wei; Zhang, Zhuoli

    2016-01-01

    This study aimed to determine the relationships between magnetic resonance elastography (MRE) imaging biomarkers and the stages of liver fibrosis in a rabbit model of liver fibrosis, a longitudinal validation for clinical translation. Liver fibrosis was induced in 38 male New Zealand rabbits by weekly subcutaneous injections of 0.1 ml 50% carbon tetrachloride oily solution per kilogram of body weight for 4 to 10 weeks to produced varying degrees of liver fibrosis. The values for the liver stiffness (LS) MRE imaging biomarkers were measured at different stages of liver fibrosis. Masson trichrome staining of liver tissue was used to identify collagen tissue. Among the 38 rabbits, the histological studies showed liver fibrosis stage 1 (F1, n = 11), liver fibrosis stage 2 (F2, n = 8), liver fibrosis stage 3 (F3, n = 7), and liver fibrosis stage 4 (F4, liver cirrhosis, n = 12). Additional healthy rabbits served as controls (F0, n = 15). During liver fibrosis progression, the mean LS values increased during liver fibrosis progression. There were significant differences in LS values between (F0 and F1) and (F2 and F3), (F2 and F3) and (F4), and (F0 and F1) and (F4), which are three clinically relevant fibrosis groups. There was a high correlation between the LS values measured by MRE and the stages of liver fibrosis determined by histology (R(2) = 0.67, P < 0.001). MRE imaging has the potential to serve as a noninvasive, unenhanced imaging technique for liver fibrosis diagnosis and staging.

  11. Magnetic resonance-guided upper abdominal biopsies in a high-field wide-bore 3-T MRI system: feasibility, handling, and needle artefacts

    Energy Technology Data Exchange (ETDEWEB)

    Kuehn, Jens-Peter; Langner, Soenke; Hegenscheid, Katrin; Hosten, Norbert; Puls, Ralf [Ernst Moritz Arndt University of Greifswald, Department of Radiology, Greifswald (Germany); Evert, Matthias [Ernst Moritz Arndt University of Greifswald, Department of Pathology, Greifswald (Germany); Kickhefel, Antje [Siemens Healthcare, Erlangen (Germany)

    2010-10-15

    To investigate the feasibility and handling of abdominal MRI-guided biopsies in a 3-T MRI system. Over a 1-year period, 50 biopsies were obtained in 47 patients with tumours of the upper abdominal organs guided by 3-T MRI with a large-bore diameter of 70 cm. Lesions in liver (47), spleen (1) and kidney (2) were biopsied with a coaxial technique using a 16-G biopsy needle guided by a T1-weighted three-dimensional gradient recalled echo volumetric interpolated breath-hold examination (T1w-3D-GRE-VIBE) sequence. Sensitivity, specificity, accuracy, complication rate, interventional complexity, room/intervention time and needle artefacts were determined. A sensitivity of 0.93, specificity of 1.0 and accuracy of 0.94 were observed. Three patients required a rebiopsy. There was a minor complications rate of 13.6%, and no major complications were observed. Histopathology revealed 38 malignant lesions, and 3-month follow-up confirmed 9 benign lesions. Mean lesion diameter was 3.4 {+-} 3.1 cm (50% being smaller than 2 cm). Mean needle tract length was 10.8 {+-} 3.3 cm. Median room time was 42.0 {+-} 19.8 min and intervention time 9.3 {+-} 8.1 min. Needle artefact size was about 9-fold greater for perpendicular access versus access parallel to the main magnetic field. Biopsies of the upper abdomen can be performed with great technical success and easy handling because of the large-bore diameter. The MRI-guided biopsy needle had an acceptable susceptibility artefact at 3 T. However future research must aim to reduce the susceptibility effects of the biopsy systems. (orig.)

  12. {sup 31}P magnetic resonance spectroscopy to measure in vivo cardiac energetics in normal myocardium and hypertrophic cardiomyopathy: Experiences at 3 T

    Energy Technology Data Exchange (ETDEWEB)

    Shivu, Ganesh Nallur [Department of Cardiovascular Medicine, University of Birmingham, Vincent Drive, Edgbaston, Birmingham B15 2TT (United Kingdom)], E-mail: drgani23@gmail.com; Abozguia, Khalid; Phan, Thanh Trung; Ahmed, Ibrar [Department of Cardiovascular Medicine, University of Birmingham, Vincent Drive, Edgbaston, Birmingham B15 2TT (United Kingdom); Henning, Anke [Institute for Biomedical Engineering, University and ETH Zurich, Gloriastrasse 35, CH-8092, Zurich CH ETZ F97 (Switzerland); Frenneaux, Michael [Department of Cardiovascular Medicine, University of Birmingham, Vincent Drive, Edgbaston, Birmingham B15 2TT (United Kingdom)

    2010-02-15

    Background: {sup 31}P magnetic resonance spectroscopy (MRS) allows measurement of in vivo high-energy phosphate kinetics in the myocardium. While traditionally {sup 31}P cardiac spectroscopy is performed at 1.5 T, cardiac MRS at higher field strength can theoretically increase signal to noise ratio (SNR) and spectral resolution therefore improving sensitivity and specificity of the cardiac spectra. The reproducibility and feasibility of performing cardiac spectroscopy at 3 T is presented here in this study in healthy volunteers and patients with hypertrophic cardiomyopathy. Methods: Cardiac spectroscopy was performed using a Phillips 3T Achieva scanner in 37 healthy volunteers and 26 patients with hypertrophic cardiomyopathy (HCM) to test the feasibility of the protocol. To test the reproducibility a single volunteer was scanned eight times on separate occasions. A single voxel {sup 31}P MRS was performed using Image Selected In vivo Spectroscopy (ISIS) volume localization. Results: The mean phosphocreatine/adenosine triphosphate (PCr/ATP) ratio of the eight measurements performed on one individual was 2.11 {+-} 0.25. Bland Altman plots showed a variance of 12% in the measurement of PCr/ATP ratios. The PCr/ATP ratio was significantly reduced in HCM patients compared to controls, 1.42 {+-} 0.51 and 2.11 {+-} 0.57, respectively, P < 0.0001. (All results are expressed as mean {+-} standard deviation). Conclusions: Here we demonstrate that cardiac {sup 31}P MRS at 3 T is a reliable method of measuring in vivo high-energy phosphate kinetics in the myocardium for clinical studies and diagnostics. Based on our data an impairment of cardiac energetic state in patients with hypertrophic cardiomyopathy is indisputable.

  13. Magnetic resonance diffusion tensor imaging (MRDTI) of the optic nerve and optic radiations at 3T in children with neurofibromatosis type I (NF-1)

    Energy Technology Data Exchange (ETDEWEB)

    Filippi, Christopher G.; Nickerson, Joshua P. [University of Vermont School of Medicine-FAHC, Department of Radiology, Burlington, VT (United States); Bos, Aaron [University of Vermont School of Medicine, Burlington, VT (United States); Salmela, Michael B. [University of Minnesota School of Medicine, Department of Radiology, Minneapolis, MN (United States); Koski, Chris J. [James Madison University, Department of Political Sciences, Harrisonburg, VA (United States); Cauley, Keith A. [University of Massachusetts Memorial Medical Center, Department of Radiology, Worcester, MA (United States)

    2012-02-15

    Optic pathway glioma (OPG) is a characteristic hallmark of neurofibromatosis type I (NF-I). To evaluate the feasibility of magnetic resonance diffusion tensor imaging (MRDTI) at 3T to detect abnormalities of the optic nerves and optic radiations in children with NF-I. 3-T MRDTI was prospectively performed in 9 children with NF-I (7 boys, 2 girls, average age 7.8 years, range 3-17 years) and 44 controls (25 boys, 19 girls, average age 8.1 years, range 3-17 years). Fractional anisotropy (FA) and mean diffusivity were determined by region-of-interest analysis for the optic nerves and radiations. Statistical analysis compared controls to NF-I patients. Two NF-I patients had bilateral optic nerve gliomas, three had chiasmatic gliomas and four had unidentified neurofibromatosis objects (UNOs) along the optic nerve pathways. All NF-I patients had statistically significant decreases in FA and elevations in mean diffusivity in the optic nerves and radiations compared to age-matched controls. MRDTI can evaluate the optic pathways in children with NF-I. Statistically significant abnormalities were detected in the diffusion tensor metrics of the optic nerves and radiations in children with NF-I compared to age-matched controls. (orig.)

  14. Three-dimensional delayed gadolinium-enhanced magnetic resonance imaging of hip joint cartilage at 3 T: A prospective controlled study

    Energy Technology Data Exchange (ETDEWEB)

    Zilkens, Christoph, E-mail: christoph.zilkens@med.uni-duesseldorf.de [Univ. Dusseldorf, Medical Faculty, Department of Orthopaedic Surgery, Moorenstrasse 5, D-40225 Dusseldorf (Germany); Miese, Falk, E-mail: falk.miese@med.uni-duesseldorf.de [Univ. Dusseldorf, Medical Faculty, Department of Diagnostic and Interventional Radiology, Moorenstrasse 5, D-40225 Dusseldorf (Germany); Kim, Young-Jo, E-mail: young-jo.kim@childrens.harvard.edu [Department of Orthopaedic Surgery, The Children' s Hospital Boston, 300 Longwood Ave., Boston, MA 02115 (United States); Hosalkar, Harish, E-mail: hhosalkar@rchsd.org [Department of Orthopaedic Surgery, Rady Children' s Hospital San Diego, 3030 Childrens Way Ste 410, San Diego, CA 92123 (United States); Antoch, Gerald, E-mail: antoch@med.uni-duesseldorf.de [Univ. Dusseldorf, Medical Faculty, Department of Diagnostic and Interventional Radiology, Moorenstrasse 5, D-40225 Dusseldorf (Germany); Krauspe, Ruediger, E-mail: krauspe@med.uni-duesseldorf.de [Univ. Dusseldorf, Medical Faculty, Department of Orthopaedic Surgery, Moorenstrasse 5, D-40225 Dusseldorf (Germany); Bittersohl, Bernd, E-mail: bbittersohl@partners.org [Univ. Dusseldorf, Medical Faculty, Department of Orthopaedic Surgery, Moorenstrasse 5, D-40225 Dusseldorf (Germany)

    2012-11-15

    Purpose: To assess acetabular and femoral hip joint cartilage with three-dimensional (3D) delayed gadolinium-enhanced magnetic resonance imaging (dGEMRIC) in patients with degeneration of hip joint cartilage and asymptomatic controls with morphologically normal appearing cartilage. Methods and materials: A total of 40 symptomatic patients (18 males, 22 females; mean age: 32.8 {+-} 10.2 years, range: 18-57 years) with different hip joint deformities including femoroacetabular impingement (n = 35), residual hip dysplasia (n = 3) and coxa magna due to Legg-Calve-Perthes disease in childhood (n = 2) underwent high-resolution 3D dGEMRIC for the evaluation of acetabular and femoral hip joint cartilage. Thirty-one asymptomatic healthy volunteers (12 males, 19 females; mean age: 24.5 {+-} 1.8 years, range: 21-29 years) without underlying hip deformities were included as control. MRI was performed at 3 T using a body matrix phased array coil. Region of interest (ROI) analyses for T1{sub Gd} assessment was performed in seven regions in the hip joint, including anterior to superior and posterior regions. Results: T1{sub Gd} mapping demonstrated the typical pattern of acetabular cartilage consistent with a higher glycosaminoglycan (GAG) content in the main weight-bearing area. T1{sub Gd} values were significantly higher in the control group than in the patient group whereas significant differences in T1{sub Gd} values corresponding to the amount of cartilage damage were noted both in the patient group and in the control group. Conclusions: Our study demonstrates the potential of high-resolution 3D dGEMRIC at 3 T for separate acetabular and femoral hip joint cartilage assessment in various forms of hip joint deformities.

  15. Age determination of vessel wall hematoma in spontaneous cervical artery dissection: A multi-sequence 3T Cardiovascular Magnetic resonance study

    Directory of Open Access Journals (Sweden)

    Habs Maximilian

    2011-11-01

    Full Text Available Abstract Background Previously proposed classifications for carotid plaque and cerebral parenchymal hemorrhages are used to estimate the age of hematoma according to its signal intensities on T1w and T2w MR images. Using these classifications, we systematically investigated the value of cardiovascular magnetic resonance (CMR in determining the age of vessel wall hematoma (VWH in patients with spontaneous cervical artery dissection (sCAD. Methods 35 consecutive patients (mean age 43.6 ± 9.8 years with sCAD received a cervical multi-sequence 3T CMR with fat-saturated black-blood T1w-, T2w- and TOF images. Age of sCAD was defined as time between onset of symptoms (stroke, TIA or Horner's syndrome and the CMR scan. VWH were categorized into hyperacute, acute, early subacute, late subacute and chronic based on their signal intensities on T1w- and T2w images. Results The mean age of sCAD was 2.0, 5.8, 15.7 and 58.7 days in patients with acute, early subacute, late subacute and chronic VWH as classified by CMR (p Conclusions Signal intensities of VWH in sCAD vary over time and multi-sequence CMR can help to determine the age of an arterial dissection. Furthermore, findings of this study suggest that the time course of carotid hematomas differs from that of cerebral hematomas.

  16. Magnetization transfer in human achilles tendon assessed by a 3D ultrashort echo time sequence. Quantitative examinations in healthy volunteers at 3T

    Energy Technology Data Exchange (ETDEWEB)

    Syha, R.; Grosse, U.; Springer, F. [Tuebingen Univ. (Germany). Diagnostic and Interventional Radiology; Tuebingen Univ. (Germany). Section on Experimental Radiology; Martirosian, P.; Schick, F. [Tuebingen Univ. (Germany). Section on Experimental Radiology; Ketelsen, D.; Claussen, C.D. [Tuebingen Univ. (Germany). Diagnostic and Interventional Radiology

    2011-11-15

    Magnetization transfer contrast (MTC) imaging provides insight into interactions between free and bounded water. Newly developed ultrashort echo time (UTE) sequences implemented on whole-body magnetic resonance (MR) scanners allow MTC imaging in tissues with extremely fast signal decay such as tendons. The aim of this study was to develop a technique for the quantification of the MT effect in healthy Achilles tendons in-vivo at 3 Tesla. 16 normal tendons of volunteers with no history of tendinopathy were examined using a 3D-UTE sequence with a rectangular on-resonant excitation pulse and a Fermi-shaped off-resonant MT preparation pulse. The frequency of the MT pulse was varied from 1 to 5 kHz. MT effects were calculated in terms of the MT ratio (MTR) between measurements without and with MT preparation. Direct saturation effects of MT preparation on the signal intensity were evaluated using numerical simulation of Bloch equations. One patient with tendinopathy was examined to exemplarily show changes of MTR under pathologic conditions. Calculation of MTR data was feasible in all examined tendons and showed a decrease from 0.53 {+-} 0.05 to 0.25 {+-} 0.03 (1 kHz to 5 kHz) for healthy volunteers. Evaluation of variation with gender and dominance of ankle revealed no significant differences (p > 0.05). In contrast, the patient with confirmed tendinopathy showed MTR values between 0.36 (1 kHz) and 0.19 (5 kHz). MT effects in human Achilles tendons can be reliably assessed in-vivo using a 3D UTE sequence at 3 T. All healthy tendons showed similar MTR values (coefficient of variation 10.0 {+-} 1.2 %). The examined patient showed a clearly different MT effect revealing a changed microstructure in the case of tendinopathy. (orig.)

  17. Metabolic changes in the anterior and posterior cingulate cortices of the normal aging brain: proton magnetic resonance spectroscopy study at 3 T.

    Science.gov (United States)

    Chiu, Pui-Wai; Mak, Henry Ka-Fung; Yau, Kelvin Kai-Wing; Chan, Queenie; Chang, Raymond Chuen-Chung; Chu, Leung-Wing

    2014-02-01

    Magnetic resonance spectroscopy (MRS) can explore aging at a molecular level. In this study, we investigated the relationships between regional concentrations of metabolites (such as choline, creatine, myo-inositol, and N-acetyl-aspartate) and normal aging in 30 cognitively normal subjects (15 women and 15 men, age range 22-82, mean = 49.9 ± 18.3 years) using quantitative proton magnetic resonance spectroscopy. All MR scans were performed using a 3 T scanner. Point resolved spectroscopy was used as the volume selection method for the region-of-interest and the excitation method for water suppression. Single voxel spectroscopy with short echo time of 39 ms and repetition time of 2,000 ms was employed. Single voxels were placed in the limbic regions, i.e., anterior cingulate cortex (ACC), posterior cingulate cortex (PCC), and left and right hippocampi. Cerebrospinal fluid normalization and T1 and T2 correction factors were implemented in the calculation of absolute metabolite concentrations. A standardized T1W 3D volumetric fast field echo and axial T2-weighted fast spin-echo images were also acquired. Our results showed significant positive correlation of choline (r = 0.545, p = 0.002), creatine (r = 0.571, p = 0.001), and N-acetyl-aspartate (r = 0.674, p age. No significant gender effect on metabolite concentrations was found. In aging, increases in choline and creatine might suggest glial proliferation, and an increase in N-acetyl-aspartate might indicate neuronal hypertrophy. Such findings highlight the metabolic changes of ACC and PCC with age, which could be compensatory to an increased energy demand coupled with a lower cerebral blood flow.

  18. A trapped magnetic field of 3 T in homogeneous, bulk MgB2 superconductors fabricated by a modified precursor infiltration and growth process

    Science.gov (United States)

    Bhagurkar, A. G.; Yamamoto, A.; Anguilano, L.; Dennis, A. R.; Durrell, J. H.; Babu, N. Hari; Cardwell, D. A.

    2016-03-01

    The wetting of boron with liquid magnesium is a critical factor in the synthesis of MgB2 bulk superconductors by the infiltration and growth (IG) process. Poor wetting characteristics can therefore result potentially in non-uniform infiltration, formation of defects in the final sample structure and poor structural homogeneity throughout the bulk material. Here we report the fabrication of near-net-shaped MgB2 bulk superconductors by a modified precursor infiltration and growth (MPIG) technique. A homogeneous bulk microstructure has subsequently been achieved via the uniform infiltration of Mg liquid by enriching pre-reacted MgB2 powder within the green precursor pellet as a wetting enhancer, leading to relatively little variation in superconducting properties across the entire bulk sample. Almost identical values of trapped magnetic field of 2.12 T have been measured at 5 K at both the top and bottom surfaces of a sample fabricated by the MPIG process, confirming the uniformity of the bulk microstructure. A maximum trapped field of 3 T has been measured at 5 K at the centre of a stack of two bulk MgB2 samples fabricated using this technique. A steady rise in trapped field was observed for this material with decreasing temperature down to 5 K without the occurrence of flux avalanches and with a relatively low field decay rate (1.5%/d). These properties are attributed to the presence of a fine distribution of residual Mg within the bulk microstructure generated by the MPIG processing technique.

  19. Interobserver and test-retest reproducibility of T1ρ and T2 mesurements of lumber intervertebral discs by 3t magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Yoo, Yeon Hwa; Yoon, Choon Sik; Eun, Na Lae; Kim, Sung Jin; Chung, Tae Sub [Dept. of Radiology, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul (Korea, Republic of); Hwang, Moon Jung [GE Health Care, Seoul (Korea, Republic of); Yoo, Hanna [Biostatistics Collaboration Lab, Yonsei University College of Medicine, Seoul (Korea, Republic of); Peter, Robert D. [GE Health Care, Milwaukee (United States); Lee, Young Han; Suh, Jin Suck [Dept. of Radiology, Severance Hospital, Yonsei University College of Medicine, Seoul (Korea, Republic of)

    2016-11-15

    To investigate the interobserver and test-retest reproducibility of T1ρ and T2 measurements of lumbar intervertebral discs using 3T magnetic resonance imaging (MRI). This study included a total of 51 volunteers (female, 26; male, 25; mean age, 54 ± 16.3 years) who underwent lumbar spine MRI with a 3.0 T scanner. Amongst these subjects, 40 underwent repeat T1ρ and T2 measurement acquisitions with identical image protocol. Two observers independently performed the region of interest measurements in the nuclei pulposi of the discs from L1-2 through L5-S1 levels. Statistical analysis was performed using intraclass correlation coefficient (ICC) with a two-way random model of absolute agreement. Comparison of the ICC values was done after acquisition of ICC values using Z test. Statistical significance was defined as p value < 0.05. The ICCs of interobserver reproducibility were 0.951 and 0.672 for T1ρ and T2 mapping, respectively. The ICCs of test-retest reproducibility (40 subjects) for T1ρ and T2 measurements were 0.922 and 0.617 for observer A and 0.914 and 0.628 for observer B, respectively. In the comparison of the aforementioned ICCs, ICCs of interobserver and test-retest reproducibility for T1ρ mapping were significantly higher than T2 mapping (p < 0.001). The interobserver and test-retest reproducibility of T1ρ mapping were significantly higher than those of T2 mapping for the quantitative assessment of nuclei pulposi of lumbar intervertebral discs.

  20. Magnetic resonance imaging in low back pain and sciatica with special emphasis on the postdiskectomy period. A study using 0.2 and 0.3 T vertical magnetic fields

    Energy Technology Data Exchange (ETDEWEB)

    Annertz, M.

    1994-09-01

    The diagnostic capability of magnetic resonance (MR) imaging at 0.3 T was evaluated and compared with other modalities in patients with previous disk surgery and remaining or recurrent sciatica, and in patients with spondylolisthesis and sciatica. In the early postoperative period after successful lumbar diskectomy, MR showed a large amount of abnormal soft tissue in the anterior epidural space, without correlation with clinical symptoms. In the late postoperative period, contrast-enhanced MR provided the best correlation with surgical findings, compared with CT and myelography, in discriminating recurrent/remaining disk herniation from epidural fibrosis in patients with the lumbar postdiskectomy syndrome. Contrast-enhanced MR could not demonstrate any differences regarding presence and extent of epidural fibrosis between symptomatic and asymptomatic patients. In spondylolisthesis, MR gave excellent information about the root canals and the degree of nerve root stretching and compression, which was not possible to evaluate with myelography. MR at 0.3 T provided information comparable to that reported from examinations performed with superconducting MR scanners, and is well suited as the imaging modality in the evaluation of lumbar spine disorders. After introducing MR at 0.2 and 0.3 T with vertical magnetic fields as the first neuroradiological modality, instead of myelography or CT, in the evaluation of the soft tissues of the lumbar spine in patients with low back pain and sciatica, a significant increase in the number of patients examined, a moderate increase in the total cost of investigations, and a significant decrease in the cost per investigated and per operated patient was noted.

  1. Quantitative circumferential strain analysis using adenosine triphosphate-stress/rest 3-T tagged magnetic resonance to evaluate regional contractile dysfunction in ischemic heart disease

    Energy Technology Data Exchange (ETDEWEB)

    Nakamura, Masashi, E-mail: m.nakamura1230@gmail.com [Department of Radiology, Ehime University Graduate School of Medicine, Shitsukawa, Toon-city, Ehime 791-0295 (Japan); Kido, Tomoyuki [Department of Radiology, Saiseikai Matsuyama Hospital, Ehime 791-0295 (Japan); Kido, Teruhito; Tanabe, Yuki; Matsuda, Takuya; Nishiyama, Yoshiko; Miyagawa, Masao; Mochizuki, Teruhito [Department of Radiology, Ehime University Graduate School of Medicine, Shitsukawa, Toon-city, Ehime 791-0295 (Japan)

    2015-08-15

    Highlights: • Infarcted segments could be differentiated from non-ischemic and ischemic segments with high sensitivity and specificity under at rest conditions. • The time-to-peak circumferential strain values in infarcted segments were more significantly delayed than those in non-ischemic and ischemic segments. • Both circumferential strain and circumferential systolic strain rate values under ATP-stress conditions were significantly lower in ischemic segments than in non-ischemic segments. • Subtracting stress and rest circumferential strain had a higher diagnostic capability for ischemia relative to only utilizing rest or ATP-stress circumferential strain values. • A circumferential strain analysis using tagged MR can quantitatively assess contractile dysfunction in ischemic and infarcted myocardium. - Abstract: Purpose: We evaluated whether a quantitative circumferential strain (CS) analysis using adenosine triphosphate (ATP)-stress/rest 3-T tagged magnetic resonance (MR) imaging can depict myocardial ischemia as contractile dysfunction during stress in patients with suspected coronary artery disease (CAD). We evaluated whether it can differentiate between non-ischemia, myocardial ischemia, and infarction. We assessed its diagnostic performance in comparison with ATP-stress myocardial perfusion MR and late gadolinium enhancement (LGE)-MR imaging. Methods: In 38 patients suspected of having CAD, myocardial segments were categorized as non-ischemic (n = 485), ischemic (n = 74), or infarcted (n = 49) from the results of perfusion MR and LGE-MR. The peak negative CS value, peak circumferential systolic strain rate (CSR), and time-to-peak CS were measured in 16 segments. Results: A cutoff value of −12.0% for CS at rest allowed differentiation between infarcted and other segments with a sensitivity of 79%, specificity of 76%, accuracy of 76%, and an area under the curve (AUC) of 0.81. Additionally, a cutoff value of 477.3 ms for time-to-peak CS at rest

  2. TTC post-processing is beneficial for functional MRI at low magnetic field: a comparative study at 1 T and 3 T.

    Science.gov (United States)

    Schwarcz, Attila; Auer, Tibor; Janszky, Jozsef; Doczi, Tamas; Merboldt, Klaus-Dietmar; Frahm, Jens

    2008-11-01

    This study aimed to broaden the diagnostic possibilities of low-field MRI systems (i) by examining the feasibility of functional MRI of human brain activation at 1 T, and (ii) by assessing its reliability in comparison with acquisitions at 3 T. Eight subjects were studied at 1 T and 3T using standard echo-planar-imaging sequences at 3-mm isotropic spatial resolution. Paradigms included silent word generation, sequential finger-to-thumb opposition, and passive finger movements. Image post-processing was carried out either with statistical parametric mapping (SPM5, single-subject and group analysis) or with a two-threshold correlation (TTC, single-subject analysis only) analysis. Single-subject analysis with SPM5 resulted in 3-5 times more activated pixels at 3 T than at 1 T in the examined Broca and sensorimotor regions. By comparison, the TTC single-subject analysis yielded the same amount of activated pixels at 3 T and 1 T. Moreover, this number was identical to that obtained with SPM at 3 T. The group analysis with SPM5 resulted in very similar numbers of activated pixels at both field strengths. The present findings suggest that a field strength of 1 T combined with adequate post-processing allows for reliable functional MRI studies of human brain activation. High-field advantages are therefore best invested in higher spatial resolution.

  3. TTC post-processing is beneficial for functional MRI at low magnetic field: a comparative study at 1 T and 3 T

    Energy Technology Data Exchange (ETDEWEB)

    Schwarcz, Attila [University of Pecs, Department of Neurosurgery, Pecs (Hungary); University of Pecs, Pecs Diagnostic Institute, Pecs (Hungary); Auer, Tibor; Doczi, Tamas [University of Pecs, Department of Neurosurgery, Pecs (Hungary); Janszky, Jozsef [University of Pecs, Department of Neurology, Pecs (Hungary); Merboldt, Klaus-Dietmar; Frahm, Jens [Biomedizinische NMR Forschungs GmbH am Max-Planck-Institut fuer biophysikalische Chemie, Goettingen (Germany)

    2008-11-15

    This study aimed to broaden the diagnostic possibilities of low-field MRI systems (i) by examining the feasibility of functional MRI of human brain activation at 1 T, and (ii) by assessing its reliability in comparison with acquisitions at 3 T. Eight subjects were studied at 1 T and 3T using standard echo-planar-imaging sequences at 3-mm isotropic spatial resolution. Paradigms included silent word generation, sequential finger-to-thumb opposition, and passive finger movements. Image post-processing was carried out either with statistical parametric mapping (SPM5, single-subject and group analysis) or with a two-threshold correlation (TTC, single-subject analysis only) analysis. Single-subject analysis with SPM5 resulted in 3-5 times more activated pixels at 3 T than at 1 T in the examined Broca and sensorimotor regions. By comparison, the TTC single-subject analysis yielded the same amount of activated pixels at 3 T and 1 T. Moreover, this number was identical to that obtained with SPM at 3 T. The group analysis with SPM5 resulted in very similar numbers of activated pixels at both field strengths. The present findings suggest that a field strength of 1 T combined with adequate post-processing allows for reliable functional MRI studies of human brain activation. High-field advantages are therefore best invested in higher spatial resolution. (orig.)

  4. A 3 T event-related functional magnetic resonance imaging (fMRI) study of primary and secondary gustatory cortex localization using natural tastants

    Energy Technology Data Exchange (ETDEWEB)

    Smits, Marion [Erasmus MC, University Medical Center Rotterdam, Department of Radiology, P.O. Box 2040, CA Rotterdam (Netherlands); K.U.Leuven, Department of Radiology, University Hospitals, Leuven (Belgium); Peeters, Ronald R.; Hecke, Paul van; Sunaert, Stefan [K.U.Leuven, Department of Radiology, University Hospitals, Leuven (Belgium)

    2007-01-15

    It is known that taste is centrally represented in the insula, frontal and parietal operculum, as well as in the orbitofrontal cortex (secondary gustatory cortex). In functional MRI (fMRI) experiments activation in the insula has been confirmed, but activation in the orbitofrontal cortex is only infrequently found, especially at higher field strengths (3 T). Due to large susceptibility artefacts, the orbitofrontal cortex is a difficult region to examine with fMRI. Our aim was to localize taste in the human cortex at 3 T, specifically in the orbitofrontal cortex as well as in the primary gustatory cortex. Event-related fMRI was performed at 3 T in seven healthy volunteers. Taste stimuli consisted of lemon juice and chocolate. To visualize activation in the orbitofrontal cortex a dedicated 3D SENSE EPI fMRI sequence was used, in addition to a 2D SENSE EPI fMRI sequence for imaging the entire brain. Data were analyzed using a perception-based model. The dedicated 3D SENSE EPI sequence successfully reduced susceptibility artefacts in the orbitofrontal area. Significant taste-related activation was found in the orbitofrontal and insular cortices. fMRI of the orbitofrontal cortex is feasible at 3 T, using a dedicated sequence. Our results corroborate findings from previous studies. (orig.)

  5. Determination of location, size, and transmurality of chronic myocardial infarction without exogenous contrast media by using cardiac magnetic resonance imaging at 3 T.

    Science.gov (United States)

    Kali, Avinash; Cokic, Ivan; Tang, Richard L Q; Yang, Hsin-Jung; Sharif, Behzad; Marbán, Eduardo; Li, Debiao; Berman, Daniel S; Dharmakumar, Rohan

    2014-05-01

    Late-gadolinium-enhanced (LGE) cardiac MRI (CMR) is a powerful method for characterizing myocardial infarction (MI), but the requisite gadolinium infusion is estimated to be contraindicated in ≈20% of patients with MI because of end-stage chronic kidney disease. The purpose of this study is to investigate whether T1 CMR obtained without contrast agents at 3 T could be an alternative to LGE CMR for characterizing chronic MIs using a canine model of MI. Canines (n=29) underwent CMR at 7 days (acute MI [AMI]) and 4 months (chronic MI [CMI]) after MI. Infarct location, size, and transmurality measured by using native T1 maps and LGE images at 1.5 T and 3 T were compared. Resolution of edema between AMI and CMI was examined with T2 maps. T1 maps overestimated infarct size and transmurality relative to LGE images in AMI (P=0.016 and P=0.007, respectively), which was not observed in CMI (P=0.49 and P=0.81, respectively) at 3 T. T1 maps underestimated infarct size and transmurality relative to LGE images in AMI and CMI (P0.20) at both field strengths. Histology showed extensive replacement fibrosis within the CMI territories. CMI detection sensitivity and specificity of T1 CMR at 3 T were 95% and 97%, respectively. Native T1 maps at 3 T can determine the location, size, and transmurality of CMI with high diagnostic accuracy. Patient studies are necessary for clinical translation. © 2014 American Heart Association, Inc.

  6. Window frame or ''superferric'' magnet design for low B(< 3T) heavy ion storage ring study

    Energy Technology Data Exchange (ETDEWEB)

    Danby, G.; DeVito, B.; Jackson, J.; Keohane, G.; Lee, Y.; Phillips, R.; Plate, S.; Repeta, L.; Skaritka, J.; Smith, L.

    1985-10-01

    Double magnets share common laminations without magnetic coupling. Single layer coils of rectangular conductor are dry wound on extruded bore tubes. Magnet construction requires no molding or prestress. Absence of superconducting (SC) magnetization fields in the aperture results in very large dynamic range. The coil is wound continuously across the midplane to give unusually large dynamic aperture. Above about2.2 T saturation is corrected by simple sextupole windings with no inductive coupling to the dipole. Ultrastable design requires no internal quench protection. A quadrupole pair of novel design gives excellent field quality to B > 2 T without corrections, with no SC magnetization. Experience shows magnets are accurate enough for the assembly to take place at its final location. No training is required. Test procedures (measurements with search coils or with the beam) and cooldown properties are discussed.

  7. Window frame or ''superferric'' magnet design for low B(<3T) heavy ion storage ring study

    Energy Technology Data Exchange (ETDEWEB)

    Danby, G.; DeVito, B.; Jackson, J.; Keohane, G., Lee, Y.; Phillips, R.; Plate, S.; Repata, L.; Skaritka, J.; Smith, L.

    1985-01-01

    Double magnets share common laminations without magnetic coupling. Single layer coils of rectangular conductor are dry wound on extruded bore tubes. Magnet construction requires no molding or prestress. Absence of superconducting (SC) magnetization fields in the aperture results in very large dynamic range. The coil is wound continuously across the modplane to give unusually large dynamic aperture. Above approx.2.2 T saturation is corrected by simple sextupole windings with no inductive coupling to the dipole. Ultrastable design requires no internal quench protection. A quadrupole pair of novel design gives excellent field quality to B > 2 T without corrections, with no SC magnetization. Experience shows magnets are accurate enough for the assembly to take place at its final location. No training is required. Test procedures (measurements with search coils or with the beam) and cooldown properties are discussed. 2 refs., 8 figs., 1 tab.

  8. Image Quality and Stenosis Assessment of Non-Contrast-Enhanced 3-T Magnetic Resonance Angiography in Patients with Peripheral Artery Disease Compared with Contrast-Enhanced Magnetic Resonance Angiography and Digital Subtraction Angiography.

    Science.gov (United States)

    Liu, Jiayi; Zhang, Nan; Fan, Zhaoyang; Luo, Nan; Zhao, Yike; Bi, Xiaoming; An, Jing; Chen, Zhong; Liu, Dongting; Wen, Zhaoying; Fan, Zhanming; Li, Debiao

    2016-01-01

    To evaluate the diagnostic performance of flow-sensitive dephasing (FSD)-prepared steady-state free precession (SSFP) magnetic resonance angiography (MRA) at 3 T for imaging infragenual arteries relative to contrast-enhanced MRA (CE-MRA) and digital subtraction angiography (DSA). A series of 16 consecutive patients with peripheral arterial disease (PAD) underwent a combined peripheral MRA protocol consisting of FSD-MRA for the calves and large field-of-view CE-MRA. DSA was performed on all patients within 1 week of the MR angiographies. Image quality and degree of stenosis was assessed by two readers with rich experience. Inter-observer agreement was determined using kappa statistics. Receiver operating characteristic (ROC) curve analysis determined the diagnostic value of FSD-MRA, CE-MRA, and CE-MRA combined with FSD-MRA (CE+FSD MRA) in predicting vascular stenosis. At the calf station, no significantly difference of subjective image quality scores was found between FSD-MRA and CE-MRA. Inter-reader agreement was excellent for both FSD-MRA and CE-MRA. Both of FSD-MRA and CE-MRA carry a stenosis overestimation risk relative to DSA standard. With DSA as the reference standard, ROC curve analysis showed that the area under the curve was largest for CE+FSD MRA. The greatest sensitivity and specificity were obtained when a cut-off stenosis score of 2 was used. In patients with severe PAD,3 T FSD-MRA provides good-quality diagnostic images without a contrast agent and is a good supplement for CE-MRA. CE+FSD MRA can improve the accuracy of vascular stenosis diagnosis.

  9. Synthesis, Characterization, and Study of In Vitro Cytotoxicity of ZnO-Fe3O4 Magnetic Composite Nanoparticles in Human Breast Cancer Cell Line (MDA-MB-231) and Mouse Fibroblast (NIH 3T3).

    Science.gov (United States)

    Bisht, Gunjan; Rayamajhi, Sagar; Kc, Biplab; Paudel, Siddhi Nath; Karna, Deepak; Shrestha, Bhupal G

    2016-12-01

    Novel magnetic composite nanoparticles (MCPs) were successfully synthesized by ex situ conjugation of synthesized ZnO nanoparticles (ZnO NPs) and Fe3O4 NPs using trisodium citrate as linker with an aim to retain key properties of both NPs viz. inherent selectivity towards cancerous cell and superparamagnetic nature, respectively, on a single system. Successful characterization of synthesized nanoparticles was done by XRD, TEM, FTIR, and VSM analyses. VSM analysis showed similar magnetic profile of thus obtained MCPs as that of naked Fe3O4 NPs with reduction in saturation magnetization to 16.63 emu/g. Also, cell viability inferred from MTT assay showed that MCPs have no significant toxicity towards noncancerous NIH 3T3 cells but impart significant toxicity at similar concentration to breast cancer cell MDA-MB-231. The EC50 value of MCPs on MDA-MB-231 is less than that of naked ZnO NPs on MDA-MB-231, but its toxicity on NIH 3T3 was significantly reduced compared to ZnO NPs. Our hypothesis for this prominent difference in cytotoxicity imparted by MCPs is the synergy of selective cytotoxicity of ZnO nanoparticles via reactive oxygen species (ROS) and exhausting scavenging activity of cancerous cells, which further enhance the cytotoxicity of Fe3O4 NPs on cancer cells. This dramatic difference in cytotoxicity shown by the conjugation of magnetic Fe3O4 NPs with ZnO NPs should be further studied that might hold great promise for the development of selective and site-specific nanoparticles. Schematic representation of the conjugation, characterization and cytotoxicity analysis of Fe3O4-ZnO magnetic composite particles (MCPs).

  10. Synthesis, Characterization, and Study of In Vitro Cytotoxicity of ZnO-Fe3O4 Magnetic Composite Nanoparticles in Human Breast Cancer Cell Line (MDA-MB-231) and Mouse Fibroblast (NIH 3T3)

    Science.gov (United States)

    Bisht, Gunjan; Rayamajhi, Sagar; KC, Biplab; Paudel, Siddhi Nath; Karna, Deepak; Shrestha, Bhupal G.

    2016-12-01

    Novel magnetic composite nanoparticles (MCPs) were successfully synthesized by ex situ conjugation of synthesized ZnO nanoparticles (ZnO NPs) and Fe3O4 NPs using trisodium citrate as linker with an aim to retain key properties of both NPs viz. inherent selectivity towards cancerous cell and superparamagnetic nature, respectively, on a single system. Successful characterization of synthesized nanoparticles was done by XRD, TEM, FTIR, and VSM analyses. VSM analysis showed similar magnetic profile of thus obtained MCPs as that of naked Fe3O4 NPs with reduction in saturation magnetization to 16.63 emu/g. Also, cell viability inferred from MTT assay showed that MCPs have no significant toxicity towards noncancerous NIH 3T3 cells but impart significant toxicity at similar concentration to breast cancer cell MDA-MB-231. The EC50 value of MCPs on MDA-MB-231 is less than that of naked ZnO NPs on MDA-MB-231, but its toxicity on NIH 3T3 was significantly reduced compared to ZnO NPs. Our hypothesis for this prominent difference in cytotoxicity imparted by MCPs is the synergy of selective cytotoxicity of ZnO nanoparticles via reactive oxygen species (ROS) and exhausting scavenging activity of cancerous cells, which further enhance the cytotoxicity of Fe3O4 NPs on cancer cells. This dramatic difference in cytotoxicity shown by the conjugation of magnetic Fe3O4 NPs with ZnO NPs should be further studied that might hold great promise for the development of selective and site-specific nanoparticles.

  11. Quantitative study of liver magnetic resonance spectroscopy quality at 3T using body and phased array coils with physical analysis and clinical evaluation.

    Directory of Open Access Journals (Sweden)

    Li Xu

    Full Text Available This study aims to investigate the quality difference of short echo time (TE breathhold 1H magnetic resonance spectroscopy (MRS of the liver at 3.0T using the body and phased array coils, respectively. In total, 20 pairs of single-voxel proton spectra of the liver were acquired at 3.0T using the phased array and body coils as receivers. Consecutive stacks of breathhold spectra were acquired using the point resolved spectroscopy (PRESS technique at a short TE of 30 ms and a repetition time (TR of 1500 ms. The first spectroscopy sequence was "copied" for the second acquisition to ensure identical voxel positioning. The MRS prescan adjustments of shimming and water suppression, signal-to noise ratio (SNR, and major liver quantitative information were compared between paired spectra. Theoretical calculation of the SNR and homogeneity of the region of interest (ROI, 2 cm×2 cm×2 cm using different coils loaded with 3D liver electromagnetic model of real human body was implemented in the theoretical analysis. The theoretical analysis showed that, inside the ROI, the SNR of the phase array coil was 2.8387 times larger than that of body coil and the homogeneity of the phase array coil and body coil was 80.10% and 93.86%, respectively. The experimental results showed excellent correlations between the paired data (all r > 0.86. Compared with the body coil group, the phased array group had slightly worse shimming effect and better SNR (all P values 0.05. The theoretical analysis and clinical experiment showed that the phased array coil was superior to the body coil with respect to 3.0T breathhold hepatic proton MRS.

  12. Follow-up of coiled intracranial aneurysms: comparison of 3D time-of-flight and contrast-enhanced magnetic resonance angiography at 3T in a large, prospective series

    Energy Technology Data Exchange (ETDEWEB)

    Pierot, Laurent [University of Reims, Department of Radiology, Maison Blanche Hospital, Reims (France); Hopital de la Maison Blanche - CHU, Service de radiologie, Reims (France); Portefaix, Christophe [University of Reims, Department of Radiology, Maison Blanche Hospital, Reims (France); University of Reims, CReSTIC SIC EA3804, Reims (France); Boulin, Anne [Foch Hospital, Department of Neuroradiology, Suresnes (France); Gauvrit, Jean-Yves [University of Rennes, Department of Radiology, CHU Rennes, Rennes (France)

    2012-10-15

    To compare 3D-TOF magnetic resonance angiography (MRA) and contrast-enhanced MRA (CE-MRA) sequences at 3T in the follow-up of coiled aneurysms with digital subtracted angiography (DSA) as the gold standard. DSA, 3D-TOF and CE-MRA were performed in a prospective series of 126 aneurysms in 96 patients (57 female, 39 male; age: 25-75 years, mean: 51.3 {+-} 11.3 years). The quality of aneurysm occlusion was assessed independently and anonymously by a core laboratory. Using DSA (gold standard technique), total occlusion was depicted in 57 aneurysms (45.2%), neck remnant in 34 aneurysms (27.0%) and aneurysm remnant in 35 aneurysms (27.8%). Sensitivity, specificity, positive predictive value and negative predictive value were very similar with 3D-TOF and CE-MRA. Visibility of coils was much better with 3D-TOF (95.2%) than with CE-MRA (23.0%) (P < 0.001). Also, substantial artefacts were less frequent with 3D-TOF (4.0%) than with CE-MRA (11.9%; P = 0.012). In this large prospective series of patients with coiled aneurysms, at 3T 3D-TOF MRA was equivalent to CE-MRA for the evaluation of aneurysm occlusion, but coil visibility was superior at 3D-TOF. Thus the use of 3D-TOF at 3T is recommended for the follow-up of coiled intracranial aneurysms. (orig.)

  13. Clinical comparison between a currently available single-loop and an investigational dual-channel endorectal receive coil for prostate magnetic resonance imaging: a feasibility study at 1.5 and 3 T.

    Science.gov (United States)

    Vos, Eline K; Sambandamurthy, Sriram; Kamel, Maged; McKenney, Robert; van Uden, Mark J; Hoeks, Caroline M A; Yakar, Derya; Scheenen, Tom W J; Fütterer, Jurgen J

    2014-01-01

    The objectives of this study were to test the feasibility of an investigational dual-channel next-generation endorectal coil (NG-ERC) in vivo, to quantitatively assess signal-to-noise ratio (SNR), and to get an impression of image quality compared with the current clinically available single-loop endorectal coil (ERC) for prostate magnetic resonance imaging at both 1.5 and 3 T. The study was approved by the institutional review board, and written informed consent was obtained from all patients. In total, 8 consecutive patients with prostate cancer underwent a local staging magnetic resonance examination with the successive use of both coils in 1 session (4 patients at 1.5 T and 4 other patients at 3 T). Quantitative comparison of both coils was performed for the apex, mid-gland and base levels at both field strengths by calculating SNR profiles in the axial plane on an imaginary line in the anteroposterior direction perpendicular to the coil surface. Two radiologists independently assessed the image quality of the T2-weighted and apparent diffusion coefficient maps calculated from diffusion-weighted imaging using a 5-point scale. Improvement of geometric distortion on diffusion-weighted imaging with the use of parallel imaging was explored. Statistical analysis included a paired Wilcoxon signed rank test for SNR and image quality evaluation as well as κ statistics for interobserver agreement. No adverse events were reported. The SNR was higher for the NG-ERC compared with the ERC up to a distance of approximately 40 mm from the surface of the coil at 1.5 T (P coils were comparable. Overall, T2-weighted image quality was considered better for NG-ERC at both field strengths. Quality of apparent diffusion coefficient maps with the use of parallel imaging was rated superior with the NG-ERC at 3 T. The investigational NG-ERC for prostate imaging outperforms the current clinically available ERC in terms of SNR and is feasible for continued development for future use as

  14. Magnetic properties and electronic structure of R{sub 3}T{sub 4}X{sub 4} (R: La-Nd, Gd-Er; T: Mn, Cu; X: Ge, Sn) compounds

    Energy Technology Data Exchange (ETDEWEB)

    Szytula, A.; Wawrzynska, E.; Penc, B.; Stuesser, N.; Tomkowicz, Z.; Zygmunt, A

    2004-03-24

    The results of magnetic and neutron diffraction studies of a new series of intermetallic compounds R{sub 3}T{sub 4}X{sub 4} (R: La-Nd, Gd-Er; T: Mn, Cu; X: Ge, Sn) are presented. These compounds crystallize in the orthorhombic Gd{sub 3}Cu{sub 4}Ge{sub 4}-type structure (space group Immm). The rare earth atoms occupy two non-equivalent crystallographic positions. All the investigated compounds are antiferromagnets. In the R{sub 3}Mn{sub 4}Sn{sub 4} series the rare earth and the manganese magnetic moments order at different temperatures: the rare earth moments order at 25 K (R: Pr) and 60 K (R: Nd), while the manganese moments order at 300 K (La), 230 K (Pr) and 210 K (Nd). The R{sub 3}Cu{sub 4}Ge{sub 4} compounds are antiferromagnets with Neel temperatures between 23 K (for Tb{sub 3}Cu{sub 4}Ge{sub 4}) and 5.9 K (for Er{sub 3}Cu{sub 4}Sn{sub 4}). For Tb{sub 3}Cu{sub 4}Ge{sub 4}, Er{sub 3}Cu{sub 4}Ge{sub 4}, Ho{sub 3}Cu{sub 4}Sn{sub 4} and Er{sub 3}Cu{sub 4}Sn{sub 4} further phase transitions occur below the Neel temperature. The magnetic moments are localized only on the rare earth atoms. The moments located at different crystallographic sites have different values and order at different temperatures. The electronic structure of Gd{sub 3}Cu{sub 4}X{sub 4} (X: Ge, Sn) was studied by X-ray photoemission spectroscopy (XPS). The complex character of the valence band influences the magnetic properties of these compounds.

  15. Noncontrast-enhanced magnetic resonance renal angiography using a repetitive artery and venous labelling technique at 3 T: comparison with contrast-enhanced magnetic resonance angiography in subjects with normal renal function

    Energy Technology Data Exchange (ETDEWEB)

    Park, Sung Yoon [Sungkyunkwan University School of Medicine, Department of Radiology and Center for Imaging Science, Samsung Medical Center, Seoul (Korea, Republic of); Severance Hospital, Yonsei University College of Medicine, Department of Radiology and Research Institute of Radiological Science, Seoul (Korea, Republic of); Kim, Chan Kyo; Park, Byung Kwan [Sungkyunkwan University School of Medicine, Department of Radiology and Center for Imaging Science, Samsung Medical Center, Seoul (Korea, Republic of); Kim, EunJu [Philips Healthcare Korea, Seoul (Korea, Republic of)

    2014-09-13

    To investigate the feasibility of noncontrast-enhanced MR angiography (NC-MRA) using the repetitive artery and venous labelling (RAVEL) technique to evaluate renal arteries compared to contrast-enhanced MR angiography (CE-MRA). Twenty-five subjects with normal renal function underwent NC-MRA using a RAVEL technique and CE-MRA at 3 T. Two independent readers analysed the MRA images. Image quality, number of renal arteries, presence or absence of an early branching vessel, and diameter of the main renal arteries were evaluated. The overall image quality of NC-MRA was fair or greater in 88 % of right and 92 % of left renal arteries, while it was 96 % in both sides with CE-MRA. On NC-MRA, the number of renal arteries in all subjects was perfectly predicted by both readers. Sensitivity and specificity for predicting early branching vessels were 82 % and 100 % for reader 1 and 82 % and 95 % for reader 2. Inter-modality agreement for comparing the diameters of main renal arteries was good or excellent at all segments for both readers. Inter-reader agreement was moderate or good at all segments except at the right distal segment on NC-MRA. NC-MRA with the RAVEL technique at 3 T may have comparable diagnostic feasibility for evaluating renal arteries compared to CE-MRA. (orig.)

  16. Comparing an accelerated 3D fast spin-echo sequence (CS-SPACE) for knee 3-T magnetic resonance imaging with traditional 3D fast spin-echo (SPACE) and routine 2D sequences

    Energy Technology Data Exchange (ETDEWEB)

    Altahawi, Faysal F.; Blount, Kevin J.; Omar, Imran M. [Northwestern University Feinberg School of Medicine, Department of Radiology, Chicago, IL (United States); Morley, Nicholas P. [Marshfield Clinic, Department of Radiology, Marshfield, WI (United States); Raithel, Esther [Siemens Healthcare GmbH, Erlangen (Germany)

    2017-01-15

    To compare a faster, new, high-resolution accelerated 3D-fast-spin-echo (3D-FSE) acquisition sequence (CS-SPACE) to traditional 2D and high-resolution 3D sequences for knee 3-T magnetic resonance imaging (MRI). Twenty patients received knee MRIs that included routine 2D (T1, PD ± FS, T2-FS; 0.5 x 0.5 x 3 mm{sup 3}; ∝10 min), traditional 3D FSE (SPACE-PD-FS; 0.5 x 0.5 x 0.5 mm{sup 3}; ∝7.5 min), and accelerated 3D-FSE prototype (CS-SPACE-PD-FS; 0.5 x 0.5 x 0.5 mm{sup 3}; ∝5 min) acquisitions on a 3-T MRI system (Siemens MAGNETOM Skyra). Three musculoskeletal radiologists (MSKRs) prospectively and independently reviewed the studies with graded surveys comparing image and diagnostic quality. Tissue-specific signal-to-noise ratios (SNR) and contrast-to-noise ratios (CNR) were also compared. MSKR-perceived diagnostic quality of cartilage was significantly higher for CS-SPACE than for SPACE and 2D sequences (p < 0.001). Assessment of diagnostic quality of menisci and synovial fluid was higher for CS-SPACE than for SPACE (p < 0.001). CS-SPACE was not significantly different from SPACE but had lower assessments than 2D sequences for evaluation of bones, ligaments, muscles, and fat (p ≤ 0.004). 3D sequences had higher spatial resolution, but lower overall assessed contrast (p < 0.001). Overall image quality from CS-SPACE was assessed as higher than SPACE (p = 0.007), but lower than 2D sequences (p < 0.001). Compared to SPACE, CS-SPACE had higher fluid SNR and CNR against all other tissues (all p < 0.001). The CS-SPACE prototype allows for faster isotropic acquisitions of knee MRIs over currently used protocols. High fluid-to-cartilage CNR and higher spatial resolution over routine 2D sequences may present a valuable role for CS-SPACE in the evaluation of cartilage and menisci. (orig.)

  17. Clinical 3T MR imaging: mastering the challenges.

    Science.gov (United States)

    Tanenbaum, Lawrence N

    2006-02-01

    3T MRI is ready to meet the needs of clinical practice. SAR limitations are minimized by technical advances and surface coils are available for all core applications. With appropriate adjustments to scanning protocols, one can master the challenges of scanning at 3T; studies of the brain, spine, chest, abdomen, pelvis, vasculature, and extremities can be consistently higher in quality than are those obtained at 1.5T. The superior studies that are obtainable at 3T have great appeal to clinicians who are sophisticated about MR technology in areas, such as neurology, orthopedics, vascular surgery, and oncology,and encourage a shift in referrals toward practices that invest in higher field technology. The greater sensitivity to magnetic susceptibility offers unique benefits in functional neuroimaging, and available software/hardware packages enhance clinical setting feasibility, which adds a source of new referrals. The greater overall signal of 3T can be manipulated to make scanning more comfortable and with less motion artifact because scan times could be half as long. Spectacular anatomic delineation that is provided by high-definition scanning at true 1024 resolution can improve preoperative assessment and may improve sensitivity to smaller lesions. 3T provides practices with an advantage that is sought increasingly by high field strength purchasers in a competitive market. Only cost considerations stand in the way of the eventual domination of 3T systems in the high field strength market.

  18. T2- and diffusion-weighted magnetic resonance imaging at 3T for the detection of prostate cancer with and without endorectal coil: An intraindividual comparison of image quality and diagnostic performance.

    Science.gov (United States)

    Baur, Alexander D J; Daqqaq, Tareef; Wagner, Moritz; Maxeiner, Andreas; Huppertz, Alexander; Renz, Diane; Hamm, Bernd; Fischer, Thomas; Durmus, Tahir

    2016-06-01

    To intraindividually compare image quality and diagnostic performance of multiparametric MRI (mpMRI) at 3T for the detection of prostate cancer (PCa) using a pelvic phased-array coil (PAC) and a combined endorectal and pelvic phased-array coil (ERC-PAC). Forty-five patients were prospectively included and received mpMRI of the prostate using a PAC and an ERC-PAC during one imaging session. Two radiologists evaluated image quality and the most suspicious lesion according to the PI-RADS scoring system. Results of MRI-TRUS-fusion biopsy of the prostate served as reference standard. Patient comfort and acceptance were assessed using a standardized questionnaire. Overall image quality for T2WI was rated significantly better with an ERC-PAC compared to a PAC (p=0.0038). The weighted kappa for PI-RADS scores for T2WI and DWI with a PAC and an ERC-PAC was 0.70 and 0.73, respectively. For a PI-RADS sum score including T2WI and DWI the area under the curve with a PAC and an ERC-PAC were 0.95-0.99 and 0.93-0.97, respectively (p=0.1395). For T2WI and DWI performed at 3T index PCa lesion identification and evaluation did not differ significantly with both coil setups. Patients preferred MRI without an ERC. Therefore, the use of an ERC may be omitted in a prostate cancer detection setting. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  19. 90°-Flip-angle three-dimensional double-echo steady-state (3D-DESS) magnetic resonance imaging of the knee: Isovoxel cartilage imaging at 3 T

    Energy Technology Data Exchange (ETDEWEB)

    Moriya, Susumu, E-mail: smoyari@yahoo.co.jp [Ishikawa Clinic, 46-1 Shimokamo-Umenoki-cho, Sakyo-ku, Kyoto-shi, Kyoto 606-0851 (Japan); Division of Health Sciences, Graduate School of Medical Science, Kanazawa University, 5-11-80, Kodatsuno, Kanazawa 920-0942 (Japan); Miki, Yukio, E-mail: yukio.miki@med.osaka-cu.ac.jp [Department of Radiology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585 (Japan); Kanagaki, Mitsunori, E-mail: mitsuk@kuhp.kyoto-u.ac.jp [Department of Diagnostic Imaging and Nuclear Medicine, Kyoto University, 54 Shogoin-kawahara-cho, Sakyo-ku, Kyoto-shi, Kyoto 606-8507 (Japan); Matsuno, Yukako, E-mail: ynoma2000jp@yahoo.co.jp [Oike Clinic, 11 Nishinokyo-Shimoai-cho, Nakagyo-ku, Kyoto-shi, Kyoto 604-8436 (Japan); Miyati, Tosiaki, E-mail: ramiyati@mhs.mp.kanazawa-u.ac.jp [Division of Health Sciences, Graduate School of Medical Science, Kanazawa University, 5-11-80, Kodatsuno, Kanazawa 920-0942 (Japan)

    2014-08-15

    Purpose: The purpose of this study was to investigate whether 3D-double echo steady state (3D-DESS) with improved contrast by setting the FA (Flip angle) at 90° is useful in 3D isotropic cartilage imaging of the knee at 3 T. Materials and methods: Imaging was performed in 10 healthy volunteers using 3 methods: with 3D-DESS using FA of 25° and 90°, and with true fast imaging with steady-state precession (True-FISP). The signal-to-noise ratio (SNR) of the synovial fluid and cartilage, and contrast-to-noise ratio (CNR) were measured, and mean values were compared. Visual assessment of artifacts was performed with the cartilage divided into 6 regions. Results: There were no significant differences in synovial fluid SNR in the comparison between FA-90° 3D-DESS and True-FISP (P = 0.364). A significantly higher cartilage SNR was observed with FA-90° 3D-DESS than with True-FISP (P = 0.031). There were no significant differences in synovial fluid-cartilage CNR between FA-90° 3D-DESS and True-FISP (P = 0.892). In the evaluation of artifacts, FA-90° 3D-DESS imaging showed a significantly higher score than True-FISP imaging in the patella and trochlea cartilage (P < 0.001, P < 0.002). Conclusions: FA-90° 3D-DESS is useful in 3D isotropic cartilage imaging of the knee at 3 T.

  20. P3T: public policies and territory

    National Research Council Canada - National Science Library

    2012-01-01

    "P3T, Public Policies and Territory is promoted by Innovació i Consultoria en Polítiques Públiques and it aims to be means for research, reflection and discussion about the relation between public policy and territories...

  1. Feasibility study of 3-T MR imaging of the skin

    Energy Technology Data Exchange (ETDEWEB)

    Aubry, Sebastien; Kastler, Bruno [CHU Jean Minjoz, Service de Radiologie A, Besancon Cedex (France); Universite de Franche-comte, Laboratoire Intervention, Imagerie, Ingenierie et Innovation en Sante, Besancon (France); Casile, Celine; Jehl, Jerome [CHU Jean Minjoz, Service de Radiologie A, Besancon Cedex (France); Humbert, Philippe [CHU Saint-Jacques, Service de Dermatologie, Besancon (France); Vidal, Chrystelle [CHU Saint-Jacques, Centre d' Investigation Clinique en Innovation technologique, Besancon (France)

    2009-07-15

    The purpose of this study was to assess the quality of 3-T magnetic resonance (MR) imaging of the skin, to describe skin anatomy at 3 T and to discuss future prospects of skin MRI. A 7-cm single-element surface receiver coil was developed for our 3-T MRI system. Thin sections were obtained with a three-dimensional FIESTA acquisition sequence and a spin-echo T1-weighted sequence (SET1). Prospective analysis was performed twice by two radiologists independently. Thirty-six healthy volunteers were included and underwent MRI on the face and the calf. Image quality was assessed regarding visibility of skin layers and quantification of artefacts. High field strength MR enables imaging of the skin with a high spatial in-plane resolution (87-180 {mu}m), the total examination lasting 15-20 min. Image quality was excellent for the calf (mean SET1 quality = 96%) with a high intra- and interobserver correlation (SET1 kappa coefficient concerning visibility of epidermis, dermis and hypodermis {>=} 0.84). Motion artefacts resulted in a small loss of quality and reproducibility for the face. In conclusion, 3-T MR allows high spatial resolution imaging of the skin and can potentially provide an accurate noninvasive means of analysing the skin. (orig.)

  2. Three-dimensional multiphase time-resolved low-dose contrast-enhanced magnetic resonance angiography using TWIST on a 32-channel coil at 3 T: a quantitative and qualitative comparison of a conventional gadolinium chelate with a high-relaxivity agent.

    Science.gov (United States)

    Giesel, Frederik L; Runge, Val; Kirchin, Miles; Mehndiratta, Amit; Gerigk, Lars; Corell, Body; von Gall, Carl; Kauczor, Hans-Ulrich; Essig, Marco

    2010-01-01

    To evaluate low-dose contrast-enhanced magnetic resonance angiography (CE-MRA) at high temporal and spatial resolution for imaging of abdominal vascular structures. Contrast-enhanced magnetic resonance angiography (TWIST [time-resolved angiography with interleaved stochastic trajectories]) was performed in 8 male New Zealand white rabbits at 3 T using a prototype 32-channel coil. Gadoteridol (Gd-HP-DO3A, ProHance; Bracco Imaging SpA, Milan, Italy) and the high-relaxivity agent gadobenate dimeglumine (Gd-BOPTA, MultiHance; Bracco Imaging SpA), each at a dose of 0.04 mmol/kg body weight, were used in an intraindividual comparison. Quantitative analysis of contrast-to-noise ratio (CNR) was performed in regions of interest placed in the aorta and the adjacent tissues. The image quality in the aorta, external iliac artery, and vena cava was categorized by 2 independent blinded readers from excellent (1) to poor (3). A significantly (P Qualitative rating showed the image quality to be excellent for gadobenate dimeglumine and adequate for gadoteridol. Time-resolved CE-MRA performed at 3 T with a 32-channel volume coil can be improved using the high-relaxivity agent gadobenate dimeglumine, which increases quality and quantity of vessel enhancement.

  3. Fuzzy logic structure analysis of trabecular bone of the calcaneus to estimate proximal femur fracture load and discriminate subjects with and without vertebral fractures using high-resolution magnetic resonance imaging at 1.5 T and 3 T.

    Science.gov (United States)

    Patel, Priyesh V; Eckstein, Felix; Carballido-Gamio, Julio; Phan, Catherine; Matsuura, Maiko; Lochmüller, Eva-Maria; Majumdar, Sharmila; Link, Thomas M

    2007-10-01

    Newly developed fuzzy logic-derived structural parameters were used to characterize trabecular bone architecture in high-resolution magnetic resonance imaging (HR-MRI) of human cadaver calcaneus specimens. These parameters were compared to standard histomorphological structural measures and analyzed concerning performance in discriminating vertebral fracture status and estimating proximal femur fracture load. Sets of 60 sagittal 1.5 T and 3.0 T HR-MRI images of the calcaneus were obtained in 39 cadavers using a fast gradient recalled echo sequence. Structural parameters equivalent to bone histomorphometry and fuzzy logic-derived parameters were calculated using two chosen regions of interest. Calcaneal, spine, and hip bone mineral density (BMD) measurements were also obtained. Fracture status of the thoracic and lumbar spine was assessed on lateral radiographs. Finally, mechanical strength testing of the proximal femur was performed. Diagnostic performance in discriminating vertebral fracture status and estimating femoral fracture load was calculated using regression analyses, two-tailed t-tests of significance, and receiver operating characteristic (ROC) analyses. Significant correlations were obtained at both field strengths between all structural and fuzzy logic parameters (r up to 0.92). Correlations between histomorphological or fuzzy logic parameters and calcaneal BMD were mostly significant (r up to 0.78). ROC analyses demonstrated that standard structural parameters were able to differentiate persons with and without vertebral fractures (area under the curve [A(Z)] up to 0.73). However, none of the parameters obtained in the 1.5-T images and none of the fuzzy logic parameters discriminated persons with and without vertebral fractures. Significant correlations were found between fuzzy or structural parameters and femoral fracture load. Using multiple regression analysis, none of the structural or fuzzy parameters were found to add discriminative value to BMD

  4. Supra-aortic low-dose contrast-enhanced time-resolved magnetic resonance (MR) angiography at 3 T: comparison with time-of-flight MR angiography and high-resolution contrast-enhanced MR angiography.

    Science.gov (United States)

    Lee, Youn-Joo; Kim, Bum-soo; Koo, Ja-Sung; Kim, Bom-Yi; Jang, Jinhee; Choi, Hyun Seok; Jung, So-Lyung; Ahn, Kook-Jin

    2015-06-01

    Low-dose, time-resolved, contrast-enhanced, magnetic resonance angiography (TR-CEMRA) has been described previously; however, a comparative study between low dose TR-CEMRA and time-of-flight MRA (TOF-MRA) in the diagnosis of supra-aortic arterial stenosis has not yet been published. To demonstrate the feasibility and effectiveness of low-dose TR-CEMRA compared with TOF-MRA, using high-resolution contrast-enhanced MRA (HR-CEMRA) as the reference standard. This prospective study consisted of 30 consecutive patients. All patients underwent TOF-MRA of the neck and circle of Willis and supra-aortic HR-CEMRA, followed by supra-aortic low-dose TR-CEMRA. Gadoterate meglumine (Gd-DOTA, Dotarem(®), Guerbet, Roissy CdG Cedex, France) was injected at a dose of 0.1 mmol/kg for HR-CEMRA, followed by a 0.03 mmol/kg bolus for low-dose TR-CEMRA. Three readers evaluated the assessibility and image quality, and then two readers classified each stenosis into the following categories: normal (0-30%), mild stenosis (31-50%), moderate (51-70%), severe (71-99%), and occlusion. TR-CEMRA and HR-CEMRA showed a greater number of assessable arterial segments than TOF-MRA (P < 0.01). For TR-CEMRA, 29 cases showed within or better than the diagnostic range, whereas all 30 cases were in the diagnostic range for TOF-MRA and HR-CEMRA. For evaluation of stenosis in a total of 743 arterial segments, both TR-CEMRA and TOF-MRA results agreed with those of HR-CEMRA in 729 segments (98.1%), with excellent inter-observer agreement of TR-CEMRA; stenosis was overestimated in nine segments (1.2%) and underestimated in five segments (0.7%). For diagnosis of stenosis using 30% as the cut-off value on HR-CEMRA, the sensitivity and specificity were 88.2% and 99.3%, respectively, for the TR-CEMRA procedure, versus 94.1% and 99.6%, respectively, for TOF-MRA. Low-dose TR-CEMRA is feasible and effective in the diagnosis of supra-aortic arterial stenosis, and could be more useful option than TOF-MRA. © The

  5. 3T MR-based treatment planning for radiotherapy of brain lesions:

    OpenAIRE

    Fallone, B Gino; Hans-Sonke, Jans; Stanescu, Teodor; Stavrev, Pavel

    2006-01-01

    Purpose. The aim of this work is to develop a complete treatment planning procedure for radiation therapy of intracranial lesions based solely on 3T magnetic resonance imaging (MRI), i.e. MRI simulation. Methods. The proposed 3T MR-based radiotherapy treatment planning procedure consists of converting the MR images into CT-like images by assigning electron density information (related to CT values) to organ structures. Firstly, the 3D distortion field present in the MR volumes is determined a...

  6. Interação de poli (oxietileno) com polieletrolitos

    OpenAIRE

    Wilhelm, Helena Maria

    1996-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina. Centro de Ciencias Fisicas e Matematicas Estudou-se a interação do poli(oxietileno) (PEO) com os polieletrólitos poli(4estirenossulfonato de sódio) (PSS) e 3,6-ioneno (ION), através de medidas espectroscópicas (FT-IR e fluorescência), viscosimétricas, térmicas e microscópicas. Os espectros de FT-IR das blendas PEO/ION não mostraram deslocamentos ou alargamento nas bandas caracteristicas dos componentes, indicativo da ausência...

  7. Hospitalidade e interação no mundo globalizado

    OpenAIRE

    Ada de Freitas Maneti Dencker

    2013-01-01

    A territorialidade e as formas básicas de interação social tem papel fundamental na comunicação e nas relações de hospitalidade no mundo globalizado. Considerando as idéias de Kant, Habermas e Derridá, o texto discute qual a hospitalidade possível diante das desigualdades e da violência presentes nas relações sociais no mundo contemporâneo. A desconstrução de Derridá da ideia d...

  8. 3T MR-Guided Brachytherapy for Gynecologic Malignancies

    CERN Document Server

    Kapur, Tina; Damato, Antonio; Schmidt, Ehud J; Viswanathan, Akila N; 10.1016/j.mri.2012.06.003

    2013-01-01

    Gynecologic malignancies are a leading cause of death in women worldwide. Standard treatment for many primary and recurrent gynecologic cancer cases includes a combination of external beam radiation, followed by brachytherapy. Magnetic Resonance Imaging (MRI) is benefitial in diagnostic evaluation, in mapping the tumor location to tailor radiation dose, and in monitoring the tumor response to treatment. Initial studies of MR-guidance in gynecologic brachtherapy demonstrate the ability to optimize tumor coverage and reduce radiation dose to normal tissues, resulting in improved outcomes for patients. In this article we describe a methodology to aid applicator placement and treatment planning for 3 Tesla (3T) MR-guided brachytherapy that was developed specifically for gynecologic cancers. This has been used in 18 cases to date in the Advanced Multimodality Image Guided Operating suite at Brigham and Women's Hospital. It is comprised of state of the art methods for MR imaging, image analysis, and treatment plann...

  9. 3D MR thermometry of frozen tissue: Feasibility and accuracy during cryoablation at 3T

    NARCIS (Netherlands)

    Overduin, C.G.; Futterer, J.J.; Scheenen, T.W.J.

    2016-01-01

    PURPOSE: To assess the feasibility and accuracy of 3D ultrashort echo time (UTE) magnetic resonance (MR) thermometry of frozen tissue during cryoablation on a clinical 3T MR system. MATERIALS AND METHODS: Ex vivo porcine muscle specimens (n = 4) were imaged during two cycles of 10:3 minutes freeze-t

  10. Application of stereological methods to estimate post-mortem brain surface area using 3T MRI

    DEFF Research Database (Denmark)

    Furlong, Carolyn; García-Fiñana, Marta; Puddephat, Michael

    2013-01-01

    The Cavalieri and Vertical Sections methods of design based stereology were applied in combination with 3 tesla (i.e. 3T) Magnetic Resonance Imaging (MRI) to estimate cortical and subcortical volume, area of the pial surface, area of the grey-white matter boundary, and thickness of the cerebral...

  11. Cortical microinfarcts on 3T MRI : Clinical correlates in memory-clinic patients

    NARCIS (Netherlands)

    Van Veluw, Susanne J.; Hilal, Saima; Kuijf, Hugo J.; Ikram, Mohammad Kamran; Xin, Xu; Yeow, Tan Boon; Venketasubramanian, Narayanaswamy; Biessels, Geert Jan; Chen, Christopher

    2015-01-01

    Introduction This is the first study to assess cerebral microinfarcts (CMIs) on 3 tesla (3T) magnetic resonance imaging (MRI) in a memory clinic population. Methods We included 238 consecutive patients (aged 72.5 ± 9.1 years) from a memory clinic in Singapore. All patients underwent extensive neurol

  12. Validation of in vivo 2D displacements from spiral cine DENSE at 3T.

    Science.gov (United States)

    Wehner, Gregory J; Suever, Jonathan D; Haggerty, Christopher M; Jing, Linyuan; Powell, David K; Hamlet, Sean M; Grabau, Jonathan D; Mojsejenko, Walter Dimitri; Zhong, Xiaodong; Epstein, Frederick H; Fornwalt, Brandon K

    2015-01-30

    Displacement Encoding with Stimulated Echoes (DENSE) encodes displacement into the phase of the magnetic resonance signal. Due to the stimulated echo, the signal is inherently low and fades through the cardiac cycle. To compensate, a spiral acquisition has been used at 1.5T. This spiral sequence has not been validated at 3T, where the increased signal would be valuable, but field inhomogeneities may result in measurement errors. We hypothesized that spiral cine DENSE is valid at 3T and tested this hypothesis by measuring displacement errors at both 1.5T and 3T in vivo. Two-dimensional spiral cine DENSE and tagged imaging of the left ventricle were performed on ten healthy subjects at 3T and six healthy subjects at 1.5T. Intersection points were identified on tagged images near end-systole. Displacements from the DENSE images were used to project those points back to their origins. The deviation from a perfect grid was used as a measure of accuracy and quantified as root-mean-squared error. This measure was compared between 3T and 1.5T with the Wilcoxon rank sum test. Inter-observer variability of strains and torsion quantified by DENSE and agreement between DENSE and harmonic phase (HARP) were assessed by Bland-Altman analyses. The signal to noise ratio (SNR) at each cardiac phase was compared between 3T and 1.5T with the Wilcoxon rank sum test. The displacement accuracy of spiral cine DENSE was not different between 3T and 1.5T (1.2 ± 0.3 mm and 1.2 ± 0.4 mm, respectively). Both values were lower than the DENSE pixel spacing of 2.8 mm. There were no substantial differences in inter-observer variability of DENSE or agreement of DENSE and HARP between 3T and 1.5T. Relative to 1.5T, the SNR at 3T was greater by a factor of 1.4 ± 0.3. The spiral cine DENSE acquisition that has been used at 1.5T to measure cardiac displacements can be applied at 3T with equivalent accuracy. The inter-observer variability and agreement of DENSE-derived peak strains and

  13. das Expectativas Sociais em Interações de Trabalho

    Directory of Open Access Journals (Sweden)

    Sônia Maria Guedes Gondim

    2008-01-01

    Full Text Available A pesquisa tem como objetivo explorar a tendência de concordar com as ações do supervisor ao se analisar uma situação de interação no trabalho. Participaram do estudo 465 estudantes universitários de Salvador e de Madrid concluintes de cursos de administração e engenharia. Através de um diálogo por escrito entre um supervisor e um empregado, analisou-se a concordância ou discordância com os seus modos de agir, e as justificativas apresentadas pelos participantes. A análise quantitativa indica que, independente da cultura, da experiência de trabalho e do curso de formação, concorda-se com o comportamento do supervisor. A análise de conteúdo qualitativa revela que as justificativas de ambos os países são similares e focam na confiança, motivação, orientação e suporte mútuos.

  14. Estabelecendo interações de ajuda com paciente hiperativo

    Directory of Open Access Journals (Sweden)

    Maria da Glória Lima

    1993-03-01

    Full Text Available O enfermeiro psiquiátrico tem expandido seu papel e vem definindo melhor suas funções utilizando-se a si próprio como instrumento terapêutico fundamentado na relação interpessoal terapêutica voltada para a pessoa que precisa de ajuda. Os objetivos deste estudo são: 1 apresentar as interações do enfermeiro com um paciente hiperativo em um hospital psiquiátrico com características custodial e terapêutica; 2 analisar o procedimento do enfermeiro à luz do referencial teórico das relações interpessoais. São apresentados trechos de 4 encontros. Da análise pudemos perceber o nível de envolvimento e comprometimento em cada encontro; observar melhora na capacidade de observação e intervenção do enfermeiro; momentos de ansiedade do paciente e do enfermeiro; momentos de intervenções inadequadas e terapêuticas.

  15. Diálogos Regulares e Interações Discordantes

    Directory of Open Access Journals (Sweden)

    Wander Emediato

    2016-07-01

    Full Text Available Este artigo possui dois objetivos básicos: discutir a especificidade das interações polêmicas como um tipo específico de diálogo regular marcado pela divergência de opiniões entre os participantes e propor uma reflexão sobre duas situações em que tais interações podem ocorrer de um modo específico: a entrevista e o debate político na televisão. Buscamos mostrar que em certas interações polêmicas não há acordo e negociação entre os participantes, mas não ocorre ruptura do diálogo entre os participantes. A esse tipo de interação damos o nome de “interação discordante”. Ela difere das interações polêmicas mais comuns, onde podem ocorrer ao mesmo tempo comportamentos concordantes e discordantes, acordos e negociações. Na interação discordante não há, no nível das opiniões e dos conteúdos expressos, nenhum acordo, negociação ou concordância, constituindo um objeto de análise interessante para o estudo das interações verbais.

  16. Hospitalidade e interação no mundo globalizado

    Directory of Open Access Journals (Sweden)

    Ada de Freitas Maneti Dencker

    2013-04-01

    Full Text Available Normal 0 21 false false false PT-BR X-NONE X-NONE MicrosoftInternetExplorer4 A territorialidade e as formas básicas de interação social tem papel fundamental na comunicação e nas relações de hospitalidade no mundo globalizado. Considerando as idéias de Kant, Habermas e Derridá, o texto discute qual a hospitalidade possível diante das desigualdades e da violência presentes nas relações sociais no mundo contemporâneo. A desconstrução de Derridá da ideia de hospitalidade cosmopolita de Kant, levantando a bandeira de uma hospitalidade incondicional, e a teoria da ação comunicativa de Habermas, em que o diálogo e a tolerância permitem uma hospitalidade condicional, permeiam o entendimento que hoje temos do caminho para o respeito ao direito de todos as pessoas, sem limites lingüísticos, territoriais, étnicos ou culturais. /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}

  17. Interações Medicamentosas Potenciais em Pacientes de Unidades de Terapia Intensiva

    Directory of Open Access Journals (Sweden)

    Liliana Batista Vieira

    2012-08-01

    Full Text Available O objetivo do estudo é determinar a frequência de interações fármaco-fármaco em prescrições de pacientes internados em unidades de terapia intensiva e analisar os fatores associados relativos à farmacoterapia e ao paciente. O estudo descritivo transversal foi realizado em dois hospitais de ensino da região centro oeste do Brasil. As interações medicamentosas potenciais foram identificadas nas prescrições do primeiro dia (24 horas e do quinto dia (120 horas de internação empregando o software Drug Reax. Dos 117 pacientes do estudo, 63,2% apresentaram interações medicamentosas potenciais nas primeiras 24 horas de internação e 68,4% pacientes em 120 horas. Houve associação estatisticamente significativa entre o número de medicamentos prescritos e a ocorrência de interações medicamentosas nos dois momentos de internação. O estudo demonstrou que a prevalência de interações fármaco-fármaco foi elevada nos hospitais investigados e que a chance de interação aumentou com o número de medicamentos prescritos, número de diagnósticos e idade maior que 60 anos. O conhecimento do mecanismo farmacológico e dos fatores de risco para interações medicamentosas potenciais contribuem para aumentar a segurança e efetividade do tratamento. Para ampliar a segurança da farmacoterapia, é essencial implementar estratégias que auxiliem a equipe de saúde a identificar as interações e implementar medidas de prevenção e monitorização de pacientes em riscos de desenvolver interações medicamentosas. A atuação do farmacêutico clínico na unidade de terapia intensiva é uma ferramenta importante para a prevenção, identificação e monitorização das interações medicamentosas.

  18. A incompletude como virtude: interação de bebês na creche

    Directory of Open Access Journals (Sweden)

    Vasconcelos Cleido Roberto Franchi e

    2003-01-01

    Full Text Available Neste artigo, mostramos algumas características dos processos interativos de crianças fundamentalmente no 1º ano de vida, através do estudo de alguns episódios de interação, recortados através do registro em vídeo, durante pesquisa em uma creche universitária, com crianças (7 a 14 meses brincando livremente. Num primeiro momento, mostramos a importância da definição de um conceito de interação que consiga captar e traduzir as características das interações ocorridas nessa faixa de idade. Também, mostramos a importância dos procedimentos metodológicos, usados para recortar o material empírico, na definição desse conceito de interação. Nessa faixa etária, os episódios de interação encontrados são bastante rápidos e desordenados e estas características são resultantes, em grande parte, do desajeitamento motor dos bebês. Esta incompletude motora, por sua vez, pode ser um elemento capaz de prolongar um episódio de interação entre crianças pequenas e/ou mesmo propiciar o surgimento de novos episódios de interação.

  19. Phosphatidylcholine induces apoptosis of 3T3-L1 adipocytes

    Directory of Open Access Journals (Sweden)

    Li Hailan

    2011-12-01

    Full Text Available Abstract Background Phosphatidylcholine (PPC formulation is used for lipolytic injection, even though its mechanism of action is not well understood. Methods The viability of 3T3-L1 pre-adipocytes and differentiated 3T3-L1 cells was measured after treatment of PPC alone, its vehicle sodium deoxycholate (SD, and a PPC formulation. Western blot analysis was performed to examine PPC-induced signaling pathways. Results PPC, SD, and PPC formulation significantly decreased 3T3-L1 cell viability in a concentration-dependent manner. PPC alone was not cytotoxic to CCD-25Sk human fibroblasts at concentrations Conclusions PPC results in apoptosis of 3T3-L1 cells.

  20. Os determinantes da interação universidade-empresa e o desenvolvimento tecnológico das empresas

    OpenAIRE

    Puffal, Daniel Pedro

    2011-01-01

    A interação entre a academia e a indústria tem sido apresentada como um importante mecanismo para o desenvolvimento tecnológico das empresas, regiões e países. Com objetivo de contribuir para a compreensão e o esclarecimento das características da interação universidade-empresa e a relação com o desempenho tecnológico das empresas brasileiras, este trabalho busca identificar se os determinantes da interação universidade-empresa e os tipos de interação influenciam no desempenho relativo à inov...

  1. MAGNET

    CERN Multimedia

    B. Curé

    The first phase of the commissioning ended in August by a triggered fast dump at 3T. All parameters were nominal, and the temperature recovery down to 4.5K was carried out in two days by the cryogenics. In September, series of ramps were achieved up to 3 and finally 3.8T, while checking thoroughly the detectors in the forward region, measuring any movement of and around the HF. After the incident of the LHC accelerator on September 19th, corrective actions could be undertaken in the forward region. When all these displacements were fully characterized and repetitive, with no sign of increments in displacement at each field ramp, it was possible to start the CRAFT, Cosmic Run at Four Tesla (which was in fact at 3.8T). The magnet was ramped up to 18.16kA and the 3 week run went smoothly, with only 4 interruptions: due to the VIP visits on 21st October during the LHC inauguration day; a water leak on the cooling demineralized water circuit, about 1 l/min, that triggered a stop of the cooling pumps, and resulte...

  2. Interação e trabalho docente em aula de alfabetização

    OpenAIRE

    Gomes-Santos,Sandoval Nonato; Jordão,Heloisa Gonçalves

    2014-01-01

    A interação em sala de aula é objeto de investigação em diferentes investimentos de pesquisa no campo dos estudos da linguagem. Para os estudos em Linguística Aplicada, entre os desafios da abordagem do tema encontra-se, conforme percepção de Kleiman (1991), a compreensão das relações entre a interação em sala de aula e problemas relativos ao processo de ensino e aprendizagem. Com base nessa percepção, este estudo propõe descrever e analisar a interação em uma sala de aula de alfabetização, a...

  3. Interação social no autismo em ambientes digitais de aprendizagem

    OpenAIRE

    Passerino, Liliana Maria; Santarosa,Lucila Costi M.

    2007-01-01

    O objetivo deste artigo é discutir sobre o desenvolvimento da interação social em ambientes digitais de aprendizagem, com sujeitos com Autismo. A revisão de literatura baseia-se na Teoria Sócio-Histórica e em teóricos do Autismo. Para tanto, parte-se de uma discussão mais ampla sobre Interação Social e seu desenvolvimento, assim como as características apresentadas no autismo, considerando a intencionalidade de comunicação como um dos principais construtos teóricos para análise da interação s...

  4. Fisioterapia geriátrica domiciliar e as interações com o lazer

    OpenAIRE

    Amanda Guiduci Marcial

    2013-01-01

    Esta pesquisa tem por objetivo discutir a interação entre Lazer e Fisioterapia no contexto do atendimento a idosos em domicílio. Para tanto, foram realizados um estudo bibliográfico e entrevistas individuais semiestruturadas com fisioterapeutas. Buscou-se elementos que colaborassem no entendimento da necessidade e da importância da interação Lazer-Fisioterapia no tocante ao campo de trabalho geriátrico domiciliar, na contextualização do tratamento proposto em favor de uma prática profissional...

  5. A interação estudante - tecnologia educacional digital em enfermagem neonatal

    OpenAIRE

    Fernanda Salim Ferreira de Castro

    2013-01-01

    É crescente o investimento de educadores em enfermagem em pesquisa e produção de objetos digitais de qualidade porque consideram que esta tecnologia oferece um futuro promissor para o processo ensino-aprendizagem. A interação homem-computador acontece através da interface, que deve ser amigável. Sabendo da importância dessa interação sentimo-nos instigadas em avaliar a interface de uma tecnologia educacional digital, junto ao usuário. Objetivo: Avaliar a interface da tecnologia educacio...

  6. Interações Medicamentosas Potenciais em Pacientes de Unidades de Terapia Intensiva

    OpenAIRE

    Liliana Batista Vieira; Adriano Max Moreira Reis; Rhanna Emanuela Fontenele Lima de Carvalho; Leila Márcia Pereira de Faria; Silvia Helena de Bortoli Cassiani

    2012-01-01

    O objetivo do estudo é determinar a frequência de interações fármaco-fármaco em prescrições de pacientes internados em unidades de terapia intensiva e analisar os fatores associados relativos à farmacoterapia e ao paciente. O estudo descritivo transversal foi realizado em dois hospitais de ensino da região centro oeste do Brasil. As interações medicamentosas potenciais foram identificadas nas prescrições do primeiro dia (24 horas) e do quinto dia (120 horas) de interna...

  7. Whole-body MR angiography with body coil acquisition at 3 T in patients with peripheral arterial disease using the contrast agent gadofosveset trisodium

    DEFF Research Database (Denmark)

    Nielsen, Yousef W; Eiberg, Jonas P; Løgager, Vibeke Berg;

    2009-01-01

    Whole-body magnetic resonance angiography (WB-MRA) at 3 T with body coil acquisition has not previously been investigated. In this study, WB-MRA was performed in this manner using the blood pool contrast agent gadofosveset trisodium.......Whole-body magnetic resonance angiography (WB-MRA) at 3 T with body coil acquisition has not previously been investigated. In this study, WB-MRA was performed in this manner using the blood pool contrast agent gadofosveset trisodium....

  8. Correlation between 3T apparent diffusion coefficient values and grading of invasive breast carcinoma.

    Science.gov (United States)

    Cipolla, Valentina; Santucci, Domiziana; Guerrieri, Daniele; Drudi, Francesco Maria; Meggiorini, Maria Letizia; de Felice, Carlo

    2014-12-01

    The aim of this study was to evaluate whether the apparent diffusion coefficient (ADC) provided by 3.0T (3T) magnetic resonance diffusion-weighted imaging (DWI) varied according to the grading of invasive breast carcinoma. A total of 92 patients with 96 invasive breast cancer lesions were enrolled; all had undergone 3T magnetic resonance imaging (MRI) for local staging. All lesions were confirmed by histological analysis, and tumor grade was established according to the Nottingham Grading System (NGS). MRI included both dynamic contrast-enhanced and DWI sequences, and ADC value was calculated for each lesion. ADC values were compared with NGS classification using the Mann-Whitney U and the Kruskal-Wallis H tests. Grading was considered as a comprehensive prognostic factor, and Rho Spearman test was performed to determine correlation between grading and tumor size, hormonal receptor status, HER2 expression and Ki67 index. Pearson's Chi square test was carried out to compare grading with the other prognostic factors. ADC values were significantly higher in G1 than in G3 tumors. No significant difference was observed when G1 and G3 were compared with G2. Tumor size, hormonal receptor status, HER2 expression and Ki67 index correlated significantly with grading but there was a significant difference only between G1 and G3 related to the ER and PR status, HER2 expression and Ki67 index. There was no statistically significant difference in lesion size between the two groups. ADC values obtained on 3T DWI correlated with low-grade (G1) and high-grade (G3) invasive breast carcinoma. 3T ADC may be a helpful tool for identifying high-grade invasive breast carcinoma. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  9. 3 T MRI in paediatrics: Challenges and clinical applications

    Energy Technology Data Exchange (ETDEWEB)

    Dagia, Charuta [Department of Medical Imaging, Royal Children' s Hospital and Murdoch Childrens Research Institute, Flemington Road, Parkville 3052 (Australia); Ditchfield, Michael [Department of Medical Imaging, Royal Children' s Hospital and Murdoch Childrens Research Institute, Flemington Road, Parkville 3052 (Australia)], E-mail: michael.ditchfield@rch.org.au

    2008-11-15

    3 T MRI is being increasingly performed for clinical purposes in paediatrics, primarily because of the potential to improve spatial and temporal resolution - these can assist in overcoming the unique anatomic, physiologic and behavioural challenges of imaging children. The increased spatial resolution improves the capacity to image small patients; with particular reference to smaller structures such as the inner ear, brachial plexus, biliary system and the vascular system. The challenges inherent to imaging at high field strength remain pertinent especially, the altered T1 contrast, artefacts (susceptibility, chemical shift and B1 inhomogeneity) and safety issues, including specific absorption rate - several of these are circumvented due to software and hardware advances, or by trade off of some of the increased signal. The above mentioned challenges also create opportunities at 3 T, with improvement in MR angiography, arterial spin labelling, functional MRI, susceptibility weighted imaging, and MR spectroscopy - all of which have distinctive applications in paediatrics. Whole body imaging also becomes more practical because of the capacity for faster scans. 3 T MRI has the potential to image all the systems in paediatrics. However, neonatal brain and paediatric spine imaging have specific challenges at 3 T. Several factors also limit cardiac imaging at present. Further improvements in coil technology and newer sequences may help overcome the challenges that remain.

  10. Quantitation of glutamate in the brain by using MR proton spectroscopy at 1.5 T and 3 T; Quantitative Bestimmung von Glutamat im Hirn mithilfe der MR-Protonenspektroskopie bei 1,5 T und 3 T

    Energy Technology Data Exchange (ETDEWEB)

    Gussew, A.; Rzanny, R.; Reichenbach, J.R. [AG Medizinische Physik, Inst. fuer Diagnostische und Interventionelle Radiologie, Friedrich Schiller Univ. Jena (Germany); Scholle, H.C. [Funktionsbereich Motorik, Pathophysiologie und Biomechanik, Klinik fuer Unfall-, Hand- und Wiederherstellungschirurgie, Friedrich Schiller Univ. Jena (Germany); Kaiser, W.A. [Inst. fuer Diagnostische und Interventionelle Radiologie, Friedrich Schiller Univ Jena (Germany)

    2008-08-15

    Purpose: the influence of different magnetic field strengths on the quantification of glutamate was experimentally investigated by means of in vitro and in vivo {sup 1}H-MR spectroscopic measurements at 1.5 T and 3 T. Materials and methods: in vitro {sup 1}H-MR measurements of aqueous solutions of NAA, glutamate, glutamine and GABA were performed on two clinical MR scanners at 1.5 T and 3 T using a single voxel PRESS sequence (TR/TE = 10000/30 ms). In vitro brain measurements were also performed at both field strengths using a PRESS 2D-{sup 1}H-CSI-sequence (TR/TE = 5000/30 ms) in 6 volunteers. Spectra at 1.5 T and 3 T were compared with respect to the overlap of the single compound spectra and the deviations between estimated and nominally adjusted concentrations. In vivo spectra at both field strengths were compared with respect to SNR{sub Glu}, line width and Cramer-Rao values of the estimated glutamate intensities by using the LCModel. For the thalamus, insular and parietal cortex mean Glu/tCr ratios were estimated and compared between 1.5 T and 3 T as well as with corresponding values in the literature. Results: in general, an improved separation of signal maxima was observed in the in vitro spectra at 3 T. Except for GABA, all in vitro concentrations estimated at 3 T revealed lower deviations from their adjusted nominal concentration compared to 1.5 T: NAA (1.5 T: -5.5%, 3 T: 0.7%), glutamate (1.5 T: -18.1%, 3 T: 12.3%), glutamine (1.5 T: 44.8%, 3 T: 9.2%), GABA (1.5 T: -24.8%, 3 T: 33.8%). The SNR of in vivo spectra at 3 T was nearly doubled compared to 1.5 T. The mean number of voxels with %SD{sub Glu} < 20 was distinctly lower at 1.5 T (53%) than at 3 T (80%). Estimated Glu/tCr ratios for thalamus, insular and parietal cortex lay in the upper range of the literature values. (orig.)

  11. Interações no ambiente de cuidado: explorando publicações de enfermagem

    Directory of Open Access Journals (Sweden)

    Gabriela Marcellino de Melo Lanzoni

    2011-06-01

    Full Text Available Revisão de literatura, utilizando a Análise Temática, com o objetivo de apreender as interações no ambiente de cuidado a partir de publicações em quatro periódicos brasileiros de enfermagem, de circulação internacional, no período de 2000 a 2007. Foi realizada leitura atenta do título, resumo e palavras-chave para seleção de textos, sendo analisados 2.548 artigos e selecionados 331. Destes, 78,6% eram pesquisas científicas e 88% apresentavam doutores na autoria. Reconheceram-se cinco unidades temáticas: atitude de interação; o processo interativo e suas finalidades; cuidado mediado e ampliado pelo processo interativo; interação prejudicada; interação como instrumento de gestão e formação do sistema de saúde. Os pesquisadores reconhecem a complexidade destas relações como fundamento do cuidado em saúde e Enfermagem.

  12. Diagnosis of moyamoya disease using 3-T MRI and MRA: value of cisternal moyamoya vessels

    Energy Technology Data Exchange (ETDEWEB)

    Sawada, Takeshi; Yamamoto, Akira; Okada, Tomohisa; Kanagaki, Mitsunori; Kasahara, Seiko; Togashi, Kaori [Kyoto University, Department of Diagnostic Imaging and Nuclear Medicine, Graduate School of Medicine, Kyoto (Japan); Miki, Yukio [Osaka City University, Department of Radiology, Graduate School of Medicine, Osaka (Japan); Kikuta, Ken-ichiro [Fukui University, Division of Neurosurgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Sciences, Fukui (Japan); Miyamoto, Susumu; Takahashi, Jun C. [Kyoto University, Department of Neurosurgery, Graduate School of Medicine, Kyoto (Japan); Fukuyama, Hidenao [Kyoto University, Human Brain Research Center, Graduate School of Medicine, Kyoto (Japan)

    2012-10-15

    The purpose of this study was to propose new magnetic resonance (MR) criteria of diagnosing moyamoya disease (MMD) from cisternal moyamoya vessels (MMVs) on 3-T magnetic resonance imaging (MRI)/magnetic resonance angiography (MRA) and compare the diagnostic accuracy of the existing MR criteria and the proposed MR criteria. Participants comprised 20 consecutive patients with MMD (4 males, 16 females) diagnosed clinically using conventional angiography and 20 controls (13 male and 7 female arteriosclerosis patients). In these participants, 3-T MRI/MRA was evaluated by the existing MR criteria, which use MMVs in the basal ganglia, and the proposed MR criteria, which use cisternal MMVs, and then these two criteria were statistically compared by McNemar's test. Diagnostic accuracy was 62.5% with the existing MR criteria and 97.5% with the proposed MR criteria. The proposed MR criteria was more sensitive (1.00) than the existing MR criteria (0.45), but less specific (0.95) than the existing MR criteria (1.00). The proposed MR criteria using cisternal MMVs showed significantly higher diagnostic accuracy than the existing MR criteria. We believe that our proposed MR criteria will be beneficial for diagnosing MMD. (orig.)

  13. Multi-tensor tractography of the motor pathway at 3T: a volunteer study.

    Science.gov (United States)

    Akter, Masuma; Hirai, Toshinori; Sasao, Akira; Nishimura, Shinichiro; Uetani, Hiroyuki; Iwashita, Koya; Yamashita, Yasuyuki

    2011-01-01

    Conventional single-tensor tractography cannot depict the entire motor tract of the corticospinal tract because of fiber-crossing and other factors. Using a 3-tesla magnetic resonance (MR) unit, we compared single- and multi-tensor methods for the tract ratio of the 5 major components of the motor pathway, the lower extremity, trunk, hand, face, and tongue, in 5 healthy volunteers. Multi-tensor tractography is better than single-tensor tractography at 3T in depicting more fibers of non-trunk areas from the primary motor cortex.

  14. Detection and characterization of unruptured intracranial aneurysms: Comparison of 3T MRA and DSA.

    Science.gov (United States)

    Mine, Benjamin; Pezzullo, Martina; Roque, Gonçalo; David, Philippe; Metens, Thierry; Lubicz, Boris

    2015-06-01

    To compare magnetic resonance angiography (MRA) at 3 Tesla (3T) and digital subtraction angiography (DSA) for the detection and characterization of unruptured intracranial aneurysms (UIA). This study has been approved by our local ethical committee. From February to August 2010, 40 consecutive patients with UIA contemporarily underwent MRA at 3T including time-of-flight (TOF-MRA) and contrast enhanced (CE-MRA) techniques and DSA. MR images were independently reviewed by 3 radiologists and DSA images were reviewed by 2 radiologists together. Interobserver and intertechnique agreements were assessed for aneurysm detection and characterization including maximal diameter, neck width and the presence of a bleb or a branch arising from the sac. DS angiography revealed 56 aneurysms. Mean sensitivity and positive predictive value of MRA were 91.4% and 93.4% respectively. For UIA measurement of UIA maximal diameter (mean κ, 0.607 and 0.601 respectively) and were moderate and fair for neck width measurement respectively (mean κ, 0.456 and 0.285 respectively). For bleb detection, intertechnique and interobserver agreements were fair and slight respectively (mean κ, 0.312 and 0.116 respectively) whereas both were slight for detection of branches arising from the sac (mean κ, 0.151 and 0.070 respectively). MR angiography at 3T has a high sensitivity for the detection of UIA. However, it remains significantly inferior to DSA for morphological characterization of UIA. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  15. 3 T MR cholangiopancreatography appearances of biliary ascariasis

    Energy Technology Data Exchange (ETDEWEB)

    Ding, Z.X., E-mail: hangzhoudzx73@126.co [Department of Radiology, Zhejiang Provincial People' s Hospital, Hangzhou (China); Yuan, J.H. [Department of Radiology, Zhejiang Provincial People' s Hospital, Hangzhou (China); Chong, V. [Department of Diagnostic Imaging, National University Health System, Yong Loo Lin School of Medicine, National University of Singapore (Singapore); Zhao, D.J. [Department of Hepatobiliary Surgery, Zhejiang Provincial People' s Hospital, Hangzhou (China); Chen, F.H.; Li, Y.M. [Department of Radiology, Zhejiang Provincial People' s Hospital, Hangzhou (China)

    2011-03-15

    Aim: To evaluate the 3 T MR cholangiopancreatography (MRCP) appearances of biliary ascariasis. Materials and methods: Nine patients with a MRCP diagnosis of biliary ascariasis were reviewed. All patients had endoscopic retrograde cholangiopancreatography (ERCP) or surgical confirmation of the disease. Results: On thin-slab MRCP imaging, Ascaris worms are clearly demarcated within the biliary tree. All the identified worms demonstrated a characteristic three-parallel-lines appearance. The middle high-signal intensity line is sandwiched between two low-signal intensity lines and they are in turn surrounded by high signal bile. On thick-slab MRCP the worms also show the three-line sign but with less clarity. However, thick-slab MRCP has the advantage of providing three-dimensional ERCP-like images of the pancreaticobiliary system. Conclusion: The 'three-line' sign appears to be a characteristic sign of biliary ascariasis on 3 T MRCP.

  16. MR safety and imaging of neuroform stents at 3T.

    Science.gov (United States)

    Nehra, Arvind; Moran, Christopher J; Cross, Dewitte T; Derdeyn, Colin P

    2004-10-01

    The Neuroform stent is a self-expanding nitinol stent designed for use in wide-necked intracranial aneurysms. Heating and imaging artifacts were evaluated by using a porcine carotid artery aneurysm model in a 3T MR system. A suspended Neuroform stent was tested for deflection. No heating was measured, and no evidence of deflection of the stent was found. Imaging artifacts were minimal. MR imaging in patients with Neuroform stent-treated aneurysms is safe and feasible.

  17. Growth stimulation of 3T3 fibroblasts by Cystatin

    Energy Technology Data Exchange (ETDEWEB)

    Quan Sun (Michigan State Univ., East Lansing (United States) Beijing Medical Univ. (China))

    1989-01-01

    Treatment of cultures of mouse 3T3 fibroblasts with Cystatin C, a thiol-proteinase inhibitor isolated from chicken egg white, resulted in an enhanced rate of cell proliferation. This stimulation was demonstrated using two independent assay systems: (a) assessment of total cell number and (b) measurement of ({sup 3}H)thymidine incorporated into acid-precipitable DNA. In both assays, the dose-response curves of Cystatin stimulation showed a rising function that plateaued at a concentration of {approximately}120 {mu}g/ml. The addition of Cystatin to cultures of Kirsten murine sarcoma virus-transformed 3T3 cells also enhanced DNA synthesis in these target cells. Control experiments showed that the presence of Cystatin did not alter the level of binding of radioactively labeled epidermal growth factor and platelet derived growth factor to 3T3 cells. These results argue against the possibility that the observed growth stimulation by Cystatin was due to growth factor contamination of the Cystatin preparation.

  18. Doppler Ultrasound Triggering for Cardiovascular MRI at 3T in a Healthy Volunteer Study.

    Science.gov (United States)

    Kording, Fabian; Yamamura, Jin; Lund, Gunnar; Ueberle, Friedrich; Jung, Caroline; Adam, Gerhard; Schoennagel, Bjoern Philip

    2017-04-10

    Electrocardiogram (ECG) triggering for cardiac magnetic resonance (CMR) may be influenced by electromagnetic interferences with increasing magnetic field strength. The aim of this study was to evaluate the performance of Doppler ultrasound (DUS) as an alternative trigger technique for CMR in comparison to ECG and pulse oximetry (POX) at 3T and using different sequence types. Balanced turbo field echo two-dimensional (2D) short axis cine CMR and 2D phase-contrast angiography of the ascending aorta was performed in 11 healthy volunteers at 3T using ECG, DUS, and POX for cardiac triggering. DUS and POX triggering were compared to the reference standard of ECG in terms of trigger quality (trigger detection and temporal variability), image quality [endocardial blurring (EB)], and functional measurements [left ventricular (LV) volumetry and aortic blood flow velocimetry]. Trigger signal detection and temporal variability did not differ significantly between ECG/DUS (I = 0.6) and ECG/POX (P = 0.4). Averaged EB was similar for ECG, DUS, and POX (pECG/DUS = 0.4, pECG/POX = 0.9). Diastolic EB was significantly decreased for DUS in comparison to ECG (P = 0.02) and POX (P = 0.04). The LV function assessment and aortic blood flow were not significantly different. This study demonstrated the feasibility of DUS for gating human CMR at 3T. The magnetohydrodynamic effect did not significantly disturb ECG triggering in this small healthy volunteer study. DUS showed a significant improvement in diastolic EB but could not be identified as a superior trigger method. The potential benefit of DUS has to be evaluated in a larger clinical patient population.

  19. Magnetizing of permanent magnets using HTS bulk magnets

    Science.gov (United States)

    Oka, Tetsuo; Muraya, Tomoki; Kawasaki, Nobutaka; Fukui, Satoshi; Ogawa, Jun; Sato, Takao; Terasawa, Toshihisa

    2012-01-01

    A demagnetized Nd-Fe-B permanent magnet was scanned just above the magnetic pole which contains the HTS bulk magnet generating a magnetic field of 3.27 T. The magnet sample was subsequently found to be fully magnetized in the open space of the static magnetic fields. We examined the magnetic field distributions when the magnetic poles were scanned twice to activate the magnet plate inversely with various overlap distances between the tracks of the bulk magnet. The magnetic field of the "rewritten" magnet reached the values of the magnetically saturated region of the material, showing steep gradients at the border of each magnetic pole. As a replacement for conventional pulse field magnetizing methods, this technique is proposed to expand the degree of freedom in the design of electromagnetic devices, and is proposed as a novel practical method for magnetizing rare-earth magnets, which have excellent magnetic performance and require intense fields of more than 3 T to be activated.

  20. Incidental pineal cysts in children who undergo 3-T MRI

    Energy Technology Data Exchange (ETDEWEB)

    Whitehead, Matthew T. [University of Tennessee Health Science Center, Department of Radiology, Memphis, TN (United States); Le Bonheur Children' s Hospital, Le Bonheur Neuroscience Institute, Memphis, TN (United States); Le Bonheur Children' s Hospital, Department of Radiology, Memphis, TN (United States); Oh, Christopher C. [University of Tennessee Health Science Center, Department of Radiology, Memphis, TN (United States); Le Bonheur Children' s Hospital, Le Bonheur Neuroscience Institute, Memphis, TN (United States); Choudhri, Asim F. [University of Tennessee Health Science Center, Department of Radiology, Memphis, TN (United States); Le Bonheur Children' s Hospital, Le Bonheur Neuroscience Institute, Memphis, TN (United States); University of Tennessee Health Science Center, Department of Neurosurgery, Memphis, TN (United States)

    2013-12-15

    Pineal cysts, both simple and complex, are commonly encountered in children. More cysts are being detected with MR technology; however, nearly all pineal cysts are benign and require no follow-up. To discover the prevalence of pineal cysts in children at our institution who have undergone high-resolution 3-T MRI. We retrospectively reviewed 100 consecutive 3-T brain MRIs in children ages 1 month to 17 years (mean 6.8 {+-} 5.1 years). We evaluated 3-D volumetric T1-W imaging, axial T2-W imaging, axial T2-W FLAIR (fluid attenuated inversion recovery) and coronal STIR (short tau inversion recovery) sequences. Pineal parenchymal and cyst volumes were measured in three planes. Cysts were analyzed for the presence and degree of complexity. Pineal cysts were present in 57% of children, with a mean maximum linear dimension of 4.2 mm (range 1.5-16 mm). Of these cysts, 24.6% showed thin septations or fluid levels reflecting complexity. None of the cysts demonstrated complete T2/FLAIR signal suppression. No cyst wall thickening or nodularity was present. There was no significant difference between the ages of children with and without cysts. Cysts were more commonly encountered in girls than boys (67% vs. 52%; P = 0.043). There was a slight trend toward increasing pineal gland volume with age. Pineal cysts are often present in children and can be incidentally detected by 3-T MRI. Characteristic-appearing pineal cysts in children are benign, incidental findings, for which follow-up is not required if there are no referable symptoms or excessive size. (orig.)

  1. Estratégias de interação no ensino do texto dissertativo

    Directory of Open Access Journals (Sweden)

    Maria Izabel de Oliveira Massoni

    2001-02-01

    Full Text Available

    Este trabalho apresenta um encaminhamento para o ensino da produção de textos dissertativos a alunos que encontram dificuldades relativas às estratégias de interação requeridas por essa tipologia. A análise de alguns problemas detectados no percurso entre a pretensão de dizer e o dito levou-nos a pensar numa prática que diminuísse tais problemas, a partir de um enfoque das habilidades de raciocínio, para o estabelecimento de premissas e das habilidades discursivas, para o engajamento a elas, por meio do trabalho com esquemas marcadores de quadros de interação social diferentes.

  2. O impacto da depressão materna nas interações iniciais

    Directory of Open Access Journals (Sweden)

    Brum, Evanisa Helena Maio

    2006-01-01

    Full Text Available O presente estudo objetiva examinar questões teóricas a respeito da depressão materna nas interações iniciais para o desenvolvimento infantil, os estudos apontam para a ocorrência de desordens comportamentais, afetivas, cognitivas e sociais, bem como alterações da atividade cerebral. Salienta-se algumas formas de intervenção precoce para dirimir a probabilidade de conseqüências adversas para o desenvolvimento infantil

  3. MR imaging of the brachial plexus: comparison between 1.5-T and 3-T MR imaging: preliminary experience

    Energy Technology Data Exchange (ETDEWEB)

    Tagliafico, Alberto; Neumaier, Carlo Emanuele; Calabrese, Massimo [National Institute for Cancer Research, Department of Radiology, Genova (Italy); Succio, Giulia; Serafini, Giovanni; Ghidara, Matteo [Santa Corona Hospital, Radiology Department, Savona (Italy); Martinoli, Carlo [Universita di Genova, Radiology Department, Genova (Italy)

    2011-06-15

    To compare 1.5-T and 3-T magnetic resonance (MR) imaging of the brachial plexus. Institutional review board approval and informed consent were obtained from 30 healthy volunteers and 30 consecutive patients with brachial plexus disturbances. MR was prospectively performed with comparable sequence parameters and coils with a 1.5-T and a 3-T system. Imaging protocols at both field strengths included T1-weighted turbo spin-echo (tSE) sequences and T2-weighed turbo spin-echo (tSE) sequences with fat saturation. The signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) between muscle and nerve were calculated for both field strengths. The visibility of brachial plexus nerve at various anatomic levels (roots, interscalene area, costoclavicular space, and axillary level) was analyzed with a four-point grading scale by two radiologists. MR imaging diagnoses and pathological findings were also compared qualitatively. SNR and CNRs were significantly higher on 3-T MR images than on 1.5-T MR images (Friedman test) for all sequences. Nerve visibility was significantly better on 3-T MR images than on 1.5-T MR images (paired sign test). Pathological findings (n = 30/30) were seen equally well with both field strengths. MR imaging diagnoses did not differ for the 1.5- and 3-T protocols. High-quality MR images of the brachial plexus can be obtained with 3-T MR imaging by using sequences similar to those used at 1.5-T MR imaging. In patients and healthy volunteers, the visibility of nerve trunks and cords at 3-T MR imaging appears to be superior to that at 1.5-T MR imaging. (orig.)

  4. Enhancement of abdominal structures on MRI at 1.5 and 3 T: a retrospective intraindividual crossover comparison

    Energy Technology Data Exchange (ETDEWEB)

    AlObaidy, Mamdoh [King Faisal Specialist Hospital and Research Center, Department of Radiology, Riyadh (Saudi Arabia); University of North Carolina at Chapel Hill, Department of Radiology, Chapel Hill, NC (United States); Ramalho, Miguel; Velloni, Fernanda; Matos, Antonio P.; Herman, Kevin; Semelka, Richard C. [University of North Carolina at Chapel Hill, Department of Radiology, Chapel Hill, NC (United States)

    2017-04-15

    To quantitatively compare the extent of enhancement of abdominal structures on MRI in an intraindividual fashion at 1.5 and 3 T. HIPAA-compliant, retrospective, longitudinal, intraindividual, crossover study, with waived informed consent, of consecutive individuals scanned at both 1.5 and 3 T closed-bore magnets using gadobenate dimeglumine during different phases of enhancement at tightly controlled arterial phase timing. Quantitative ROI measurements and qualitative sub-phase arterial phase assignments were independently performed by two radiologists. Qualitative discrepancies were resolved by a senior radiologist. Final population included 60 patients [41 female and 19 male; age, 49.35 ± 18.31 years (range 16-81); weight, 78.88 ± 20.3 kg (range 44.5-136)]. Similar enhancement peak patterns were noted at both field strengths. Interobserver agreement of quantitative evaluations was substantial. Significantly higher amplitudes of enhancement peaks were noted for all abdominal solid organs during all phases at 3 T, except for the pancreas (p = 0.17-0.30). Significantly higher amplitudes of enhancement peaks of the abdominal aorta at 1.5 T were noted. Similar peak patterns of enhancement for abdominal structures were observed at 1.5 and 3 T, with solid abdominal organs showing a higher percentage enhancement at 3 T, while unexpectedly higher aortic higher percentage enhancement was observed at 1.5 T. (orig.)

  5. High-resolution intracranial vessel wall MRI in an elderly asymptomatic population: comparison of 3T and 7T

    Energy Technology Data Exchange (ETDEWEB)

    Harteveld, Anita A.; Kolk, Anja G. van der; Dieleman, Nikki; Siero, Jeroen C.W.; Luijten, Peter R.; Zwanenburg, Jaco J.M.; Hendrikse, Jeroen [University Medical Center Utrecht, Department of Radiology, Postbox 85500, Utrecht (Netherlands); Worp, H.B. van der; Frijns, Catharina J.M. [University Medical Center Utrecht, Department of Neurology and Neurosurgery, Brain Center Rudolf Magnus, Utrecht (Netherlands); Kuijf, Hugo J. [University Medical Center Utrecht, Image Sciences Institute, Utrecht (Netherlands)

    2017-04-15

    Several intracranial vessel wall sequences have been described in recent literature, with either 3-T or 7-T magnetic resonance imaging (MRI). In the current study, we compared 3-T and 7-T MRI in visualising both the intracranial arterial vessel wall and vessel wall lesions. Twenty-one elderly asymptomatic volunteers were scanned by 3-T and 7-T MRI with an intracranial vessel wall sequence, both before and after contrast administration. Two raters scored image quality, and presence and characteristics of vessel wall lesions. Vessel wall visibility was equal or significantly better at 7 T for the studied arterial segments, even though there were more artefacts hampering assessment. The better visualisation of the vessel wall at 7 T was most prominent in the proximal anterior cerebral circulation and the posterior cerebral artery. In the studied elderly asymptomatic population, 48 vessel-wall lesions were identified at 3 T, of which 7 showed enhancement. At 7 T, 79 lesions were identified, of which 29 showed enhancement. Seventy-one percent of all 3-T lesions and 59 % of all 7-T lesions were also seen at the other field strength. Despite the large variability in detected lesions at both field strengths, we believe 7-T MRI has the highest potential to identify the total burden of intracranial vessel wall lesions. (orig.)

  6. As interações e brincadeiras na educação infantil

    Directory of Open Access Journals (Sweden)

    Marleide de Lima Silva

    2014-07-01

    Full Text Available Este artigo é resultado de uma pesquisa que teve como objetivo compreender a concepção dos professores em relação às interações e brincadeiras na Educação Infantil, numa instituição pública do município de Sinop, Mato Grosso. Trata-se de pesquisa qualitativa, embasada nos teóricos Lev Vygotsky e Tizuko Kishimoto. Verificamos que, apesar dos esforços da instituição para se adequar a legislação pertinente em vigor e de haver a compreensão de que as interações e as brincadeiras são atividades eficazes para o desenvolvimento da criança, há problemas de infra-estrutura e recursos didáticos para o atendimento das necessidades das crianças.Palavras-chave: educação infantil; interações; brincadeiras.

  7. Aspartame downregulates 3T3-L1 differentiation.

    Science.gov (United States)

    Pandurangan, Muthuraman; Park, Jeongeun; Kim, Eunjung

    2014-10-01

    Aspartame is an artificial sweetener used as an alternate for sugar in several foods and beverages. Since aspartame is 200 times sweeter than traditional sugar, it can give the same level of sweetness with less substance, which leads to lower-calorie food intake. There are reports that consumption of aspartame-containing products can help obese people lose weight. However, the potential role of aspartame in obesity is not clear. The present study investigated whether aspartame suppresses 3T3-L1 differentiation, by downregulating phosphorylated peroxisome proliferator-activated receptor γ (p-PPARγ), peroxisome proliferator-activated receptor γ (PPARγ), fatty acid-binding protein 4 (FABP4), CCAAT/enhancer-binding protein α (C/EBPα), and sterol regulatory element-binding protein 1 (SREBP1), which are critical for adipogenesis. The 3T3-L1 adipocytes were cultured and differentiated for 6 d in the absence and presence of 10 μg/ml of aspartame. Aspartame reduced lipid accumulation in differentiated adipocytes as evidenced by Oil Red O staining. qRT-PCR analysis showed that the PPARγ, FABP4, and C/EBPα mRNA expression was significantly reduced in the aspartame-treated adipocytes. Western blot analysis showed that the induction of p-PPARγ, PPARγ, SREBP1, and adipsin was markedly reduced in the aspartame-treated adipocytes. Taken together, these data suggest that aspartame may be a potent substance to alter adipocyte differentiation and control obesity.

  8. Cannabidiol promotes browning in 3T3-L1 adipocytes.

    Science.gov (United States)

    Parray, Hilal Ahmad; Yun, Jong Won

    2016-05-01

    Recruitment of the brown-like phenotype in white adipocytes (browning) and activation of existing brown adipocytes are currently being investigated as a means to combat obesity. Thus, a wide variety of dietary agents that contribute to browning of white adipocytes have been identified. The present study was designed to investigate the effects of cannabidiol (CBD), a major nonpsychotropic phytocannabinoid of Cannabis sativa, on induction of browning in 3T3-L1 adipocytes. CBD enhanced expression of a core set of brown fat-specific marker genes (Ucp1, Cited1, Tmem26, Prdm16, Cidea, Tbx1, Fgf21, and Pgc-1α) and proteins (UCP1, PRDM16, and PGC-1α). Increased expression of UCP1 and other brown fat-specific markers contributed to the browning of 3T3-L1 adipocytes possibly via activation of PPARγ and PI3K. In addition, CBD increased protein expression levels of CPT1, ACSL, SIRT1, and PLIN while down-regulating JNK2, SREBP1, and LPL. These data suggest possible roles for CBD in browning of white adipocytes, augmentation of lipolysis, thermogenesis, and reduction of lipogenesis. In conclusion, the current data suggest that CBD plays dual modulatory roles in the form of inducing the brown-like phenotype as well as promoting lipid metabolism. Thus, CBD may be explored as a potentially promising therapeutic agent for the prevention of obesity.

  9. Eventos adversos por interações medicamentosas potenciais em unidade de terapia intensiva de um hospital de ensino

    Directory of Open Access Journals (Sweden)

    Mariana Macedo Alvim

    2015-12-01

    Full Text Available RESUMO Objetivo: Avaliar a existência de interações medicamentosas potenciais na unidade de terapia intensiva de um hospital, com foco nos antimicrobianos. Métodos: Estudo transversal, que analisou prescrições eletrônicas de pacientes da unidade de terapia intensiva de um hospital de ensino, avaliando potenciais interações medicamentosas relacionadas aos antimicrobianos, entre 1º de janeiro e 31 de março de 2014. O consumo dos antimicrobianos foi expresso em dose diária definida por 100 pacientes-dia. A busca e a classificação das interações foram realizadas com base no sistema Micromedex®. Resultados: Foram analisadas prescrições diárias de 82 pacientes, totalizando 656 prescrições. Do total de medicamentos prescritos, 25% eram antimicrobianos, sendo meropenem, vancomicina e ceftriaxona os mais prescritos. Os antimicrobianos mais consumidos, segundo a metodologia de dose diária definida por 100 pacientes-dia, foram cefepime, meropenem, sulfametoxazol + trimetoprima e ciprofloxacino. A média de interações por paciente foi de 2,6. Entre as interações, 51% foram classificadas como contraindicadas ou de gravidade importante. Destacaram-se as interações altamente significativas (valor clínico 1 e 2, com prevalência de 98%. Conclusão: Com o presente trabalho verifica-se que os antimicrobianos são uma classe frequentemente prescrita na unidade de terapia intensiva, apresentando elevada quantidade de interações medicamentosas potenciais, sendo a maior parte das interações considerada altamente significativa.

  10. Correlation between 3 T apparent diffusion coefficient values and grading of invasive breast carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Cipolla, Valentina, E-mail: valentina.cipolla@yahoo.it [Department of Radiological Sciences, University of Rome “Sapienza”, Viale del Policlinico 155, 00161 Rome (Italy); Santucci, Domiziana; Guerrieri, Daniele; Drudi, Francesco Maria [Department of Radiological Sciences, University of Rome “Sapienza”, Viale del Policlinico 155, 00161 Rome (Italy); Meggiorini, Maria Letizia [Department of Gynaecological Sciences, University of Rome “Sapienza”, Viale del Policlinico 155, 00161 Rome (Italy); Felice, Carlo de [Department of Radiological Sciences, University of Rome “Sapienza”, Viale del Policlinico 155, 00161 Rome (Italy)

    2014-12-15

    Highlights: • Apparent diffusion coefficient is a quantitative parameter which reflects molecular water movement. • Grading is an independent prognostic factor which correlates with other histopathological features. • Apparent diffusion coefficient values were significantly different between G1 and G3 classes. - Abstract: Purpose: The aim of this study was to evaluate whether the apparent diffusion coefficient (ADC) provided by 3.0 T (3 T) magnetic resonance diffusion-weighted imaging (DWI) varied according to the grading of invasive breast carcinoma. Materials and methods: A total of 92 patients with 96 invasive breast cancer lesions were enrolled; all had undergone 3 T magnetic resonance imaging (MRI) for local staging. All lesions were confirmed by histological analysis, and tumor grade was established according to the Nottingham Grading System (NGS). MRI included both dynamic contrast-enhanced and DWI sequences, and ADC value was calculated for each lesion. ADC values were compared with NGS classification using the Mann–Whitney U and the Kruskal–Wallis H tests. Grading was considered as a comprehensive prognostic factor, and Rho Spearman test was performed to determine correlation between grading and tumor size, hormonal receptor status, HER2 expression and Ki67 index. Pearson's Chi square test was carried out to compare grading with the other prognostic factors. Results: ADC values were significantly higher in G1 than in G3 tumors. No significant difference was observed when G1 and G3 were compared with G2. Tumor size, hormonal receptor status, HER2 expression and Ki67 index correlated significantly with grading but there was a significant difference only between G1 and G3 related to the ER and PR status, HER2 expression and Ki67 index. There was no statistically significant difference in lesion size between the two groups. Conclusion: ADC values obtained on 3 T DWI correlated with low-grade (G1) and high-grade (G3) invasive breast carcinoma. 3

  11. Acute vertigo in an anesthesia provider during exposure to a 3T MRI scanner

    Directory of Open Access Journals (Sweden)

    Gorlin A

    2015-03-01

    Full Text Available Andrew Gorlin,1 Joseph M Hoxworth,2 William Pavlicek,2 Christopher A Thunberg,1 David Seamans1 1Department of Anesthesiology, 2Department of Radiology, Mayo Clinic Arizona, Phoenix, AZ, USA Abstract: Vertigo induced by exposure to the magnetic field of a magnetic resonance imaging (MRI scanner is a well-known phenomenon within the radiology community but is not widely appreciated by other clinical specialists. Here, we describe a case of an anesthetist experiencing acute vertigo while providing sedation to a patient undergoing a 3 Tesla MRI scan. After discussing previous reports, and the evidence surrounding MRI-induced vertigo, we review potential etiologies that include the effects of both static and time-varying magnetic fields on the vestibular apparatus. We conclude our review by discussing the occupational standards that exist for MRI exposure and methods to minimize the risks of MRI-induced vertigo for clinicians working in the MRI environment. Keywords: occupational medicine, MRI worker safety, vestibular dysfunction, magnetic field, 3T MRI scanner

  12. Uniform DT 3T burn: computations and sensitivities

    Energy Technology Data Exchange (ETDEWEB)

    Vold, Erik [Los Alamos National Laboratory; Hryniw, Natalia [Los Alamos National Laboratory; Hansen, Jon A [Los Alamos National Laboratory; Kesler, Leigh A [Los Alamos National Laboratory; Li, Frank [Los Alamos National Laboratory

    2011-01-27

    A numerical model was developed in C to integrate the nonlinear deutrium-tritium (DT) burn equations in a three temperature (3T) approximation for spatially uniform test problems relevant to Inertial Confinement Fusion (ICF). Base model results are in excellent agreement with standard 3T results. Data from NDI, SESAME, and TOPS databases is extracted to create fits for the reaction rate parameter, the Planck opacity, and the coupling frequencies of the plasma temperatures. The impact of different fits (e.g., TOPS versus SESAME opacity data, higher order polynomial fits ofNDI data for the reaction rate parameter) were explored, and sensitivity to several model inputs are presented including: opacity data base, Coulomb logarithm, and Bremsstrahlung. Sensitivity to numerical integration time step size, and the relative insensitivity to the discretized numerics and numerical integration method was demonstrated. Variations in the IC for densities and temperatures were explored, showing similar DT burn profiles in most cases once ignition occurs. A coefficient multiplying the Compton coupling term (default, A = 1) can be adjusted to approximate results from more sophisticated models. The coefficient was reset (A = 0.4) to match the maximum temperatures resulting from standard multi-group simulations of the base case test problem. Setting the coefficient to a larger value, (A = 0.6) matches maximum ion temperatures in a kinetic simulation of a high density ICF-like regime. Matching peak temperatures does not match entire temperature-time profiles, indicating the Compton coefficient is density and time dependent as the photon distribution evolves. In the early time burn during the ignition of the DT, the present model with modified Compton coupling provides a very simple method to obtain a much improved match to the more accurate solution from the multi-group radiation model for these DT burn regimes.

  13. 3D-Flair sequence at 3T in cochlear otosclerosis

    Energy Technology Data Exchange (ETDEWEB)

    Lombardo, Francesco; De Cori, Sara; Aghakhanyan, Gayane; Montanaro, Domenico; De Marchi, Daniele; Frijia, Francesca; Canapicchi, Raffaello [Fondazione CNR Regione Toscana ' ' G. Monasterio' ' , Neuroradiology Unit, Pisa (Italy); Fortunato, Susanna; Forli, Francesca; Berrettini, Stefano [University of Pisa, ENT Audiology Phoniatry Unit, Department of Neuroscience, Pisa (Italy); Chiappino, Dante [Fondazione CNR Regione Toscana ' ' G. Monasterio' ' , Department of Radiology, Massa (Italy)

    2016-10-15

    To assess the capability of three-dimensional fluid-attenuated inversion recovery (3D-FLAIR) sequences in detecting signal alterations of the endolabyrinthine fluid in patients with otosclerosis. 3D-FLAIR before and after (-/+) gadolinium (Gd) administration was added to the standard MR protocol and acquired in 13 patients with a clinical/audiological diagnosis of severe/profound hearing loss in otosclerosis who were candidates for cochlear implantation and in 11 control subjects using 3-T magnetic resonance imaging (MRI) equipment. The MRI signal of the fluid-filled cochlea was assessed both visually and calculating the signal intensity ratio (SIR = signal intensity cochlea/brainstem). We revealed no endocochlear signal abnormalities on T1-weighted -/+ Gd images for either group, while on 3D-FLAIR we found bilateral hyperintensity with enhancement after Gd administration in eight patients and bilateral hyperintensity without enhancement in one patient. No endocochlear signal abnormalities were detected in other patients or the control group. Using 3-T MRI equipment, the 3D-FLAIR -/+ Gd sequence is able to detect the blood-labyrinth barrier (BLB) breakdown responsible for alterations of the endolabyrinthine fluid in patients with cochlear otosclerosis. We believe that 3D-FLAIR +/- Gd is an excellent imaging modality to assess the intra-cochlear damage in otosclerosis patients. (orig.)

  14. Design, Manufacture and Test of a 1.3 T / 10 Hz dipole model for Rapid Cycling Synchrotrons

    CERN Document Server

    Newborough, A

    2013-01-01

    The construction of a compact rapid cycling synchrotron has recently been studied at CERN to replace the first stage of its accelerator complex, the proton synchrotron booster. Although currently there are no plans to build this machine, fast cycled accelerator magnets are of general interest for numerous reasons. This has led to the design, manufacture and testing of a scaled model dipole as detailed in this paper to show the capability of producing and characterising a magnet design based on high-silicon content grain-oriented steel able to operate up to 1.3 T at 10 Hz.

  15. Right coronary MR angiography at 7 T: a direct quantitative and qualitative comparison with 3 T in young healthy volunteers.

    Science.gov (United States)

    van Elderen, Saskia G C; Versluis, Maarten J; Westenberg, Jos J M; Agarwal, Harsh; Smith, Nadine B; Stuber, Matthias; de Roos, Albert; Webb, Andrew G

    2010-10-01

    To objectively compare quantitative parameters related to image quality attained at coronary magnetic resonance (MR) angiography of the right coronary artery (RCA) performed at 7 T and 3 T. Institutional review board approval was obtained, and volunteers provided signed informed consent. Ten healthy adult volunteers (mean age ± standard deviation, 25 years ± 4; seven men, three women) underwent navigator-gated three-dimensional MR angiography of the RCA at 7 T and 3 T. For 7 T, a custom-built quadrature radiofrequency transmit-receive surface coil was used. At 3 T, a commercial body radiofrequency transmit coil and a cardiac coil array for signal reception were used. Segmented k-space gradient-echo imaging with spectrally selective adiabatic fat suppression was performed, and imaging parameters were similar at both field strengths. Contrast-to-noise ratio between blood and epicardial fat; signal-to-noise ratio of the blood pool; RCA vessel sharpness, diameter, and length; and navigator efficiency were quantified at both field strengths and compared by using a Mann-Whitney U test. The contrast-to-noise ratio between blood and epicardial fat was significantly improved at 7 T when compared with that at 3 T (87 ± 34 versus 52 ± 13; P = .01). Signal-to-noise ratio of the blood pool was increased at 7 T (109 ± 47 versus 67 ± 19; P = .02). Vessel sharpness obtained at 7 T was also higher (58% ± 9 versus 50% ± 5; P = .04). At the same time, RCA vessel diameter and length and navigator efficiency showed no significant field strength-dependent difference. In our quantitative and qualitative study comparing in vivo human imaging of the RCA at 7 T and 3 T in young healthy volunteers, parameters related to image quality attained at 7 T equal or surpass those from 3 T.

  16. Six new chalcones from Angelica keiskei inducing adiponectin production in 3T3-L1 adipocytes.

    Science.gov (United States)

    Ohnogi, Hiromu; Kudo, Yoko; Tahara, Kenichi; Sugiyama, Katsumi; Enoki, Tatsuji; Hayami, Shoko; Sagawa, Hiroaki; Tanimura, Yuko; Aoi, Wataru; Naito, Yuji; Kato, Ikunoshin; Yoshikawa, Toshikazu

    2012-01-01

    Angelica keiskei (Ashitaba in Japanese), a traditional herb in Japan, contains abundant prenylated chalcones. It has been reported that the chalcones from A. keiskei showed such bioactivities as anti-bacterial, anti-cancer and anti-diabetic effects. Xanthoangelol, 4-hydroxyderricin and six new chalcones were isolated in this study from an ethanol extract of A. keiskei by octadecyl silyl (ODS) and silica gel chromatography, and identified by 1D- and 2D-nuclear magnetic resonance (NMR) and high-resolution mass spectrometric analyses. The chalcones from A. keiskei markedly increased the expression of the adiponectin gene and the production of adiponectin in 3T3-L1 adipocytes. These results suggest that the chalcones from A. keiskei might be useful for preventing the metabolic syndrome.

  17. Can MRI biomarkers at 3 T identify low-risk ductal carcinoma in situ?

    Science.gov (United States)

    Rahbar, Habib; Parsian, Sana; Lam, Diana L; Dontchos, Brian N; Andeen, Nicole K; Rendi, Mara H; Lehman, Constance D; Partridge, Savannah C

    2016-01-01

    The objective was to explore whether 3-T magnetic resonance imaging (MRI) can identify low-risk ductal carcinoma in situ (DCIS). Dynamic contrast-enhanced and diffusion-weighted (DWI) MRI features of 36 DCIS lesions [8 low risk, Van Nuys Pathologic Classification (VNPC) 1; 28 high risk, VNPC 2/3] were reviewed. An MRI model that best identified low-risk DCIS was determined using multivariate logistic regression. Low-risk DCIS exhibited different DWI properties [i.e., higher contrast-to-noise ratio (P=.02) and lower normalized apparent diffusion coefficients (P=.04)] than high-risk DCIS. A model combining these DWI features provided best performance (area under receiver operating characteristic curve =0.86). DWI may help identify DCIS lesions requiring less therapy. Copyright © 2015 Elsevier Inc. All rights reserved.

  18. The value of high-field MRI (3 T) in the assessment of sellar lesions

    Energy Technology Data Exchange (ETDEWEB)

    Pinker, K. [Department of Diagnostic Radiology, Medical University Vienna, Waehringer Guertel 18-20, 1090 Vienna (Austria); Ba-Ssalamah, A. [Department of Diagnostic Radiology, Medical University Vienna, Waehringer Guertel 18-20, 1090 Vienna (Austria); Wolfsberger, S. [Department of Neurosurgery, Medical University Vienna (Austria); Mlynarik, V. [Department of Diagnostic Radiology, Medical University Vienna, Waehringer Guertel 18-20, 1090 Vienna (Austria); Knosp, E. [Department of Neurosurgery, Medical University Vienna (Austria); Trattnig, S. [Department of Diagnostic Radiology, Medical University Vienna, Waehringer Guertel 18-20, 1090 Vienna (Austria)]. E-mail: siegfried.trattnig@univie.ac.at

    2005-06-01

    The aim of this study was the evaluation of the normal sellar anatomy in vitro and in vivo with high-field MRI and its application in the diagnosis of sellar pathologies in comparison to standard MRI. All high-field MR images were obtained using a 3 T Bruker Medspec 30/80 Scanner with a head birdcage transmit/receive coil and an actively shielded gradient system with a maximum gradient strength of 45 mT/m. Firstly an in vitro study of the sella turcica was performed to depict normal pituitary and sellar anatomy at high field. After a pilot-study this sequence-protocol was established: A RARE sequence (TR/TE = 7790/19 ms; matrix size, 512 x 512; RARE factor = 8, FOV, 200 mm) was used for T2-weighted coronal, axial and sagittal images. A 3D gradient echo sequence with magnetization-preparation (MP-RAGE, TR/TE/TI 33.5/7.6/800 ms, matrix size, 512 x 512; FOV, 200 mm, effective slice thickness, 1.88 mm; 3 averages) was used for acquisition of T1-weighted pre- and post-contrast images. Between January 2002 and March 200458 patients were enrolled in this study. Seven patients were examined for suspected microadenoma and in 51 patients 3T MRI was used to obtain additional information about the sellar lesion already known to be present from standard MRI. In 21 cases the accuracy of the imaging findings was assessed afterwards by comparison with intraoperative findings. The infiltration of the medial cavernous sinus wall was suspected on standard MRI on 15 sides (47%), on high-field MRI on 9 sides (28%) and could be verified by intraoperative findings on 6 sides (19%). Accordingly, sensitivity to infiltration was 83% for 3 T and 67% for standard MRI. Specificity was 84% for 3 T and 58% for standard MRI. Moreover, high-field MRI revealed microadenomas in 7 patients with a median diameter of 4 mm (range 2-9 mm). The segments of the cranial nerves were seen as mean 4 hypointense spots (range 2-5 spots) on high-field MRI in contrast to 3 spots (range 0-4 spots) on standard MRI

  19. Interação Humano - Computador usando Visão Computacional

    Directory of Open Access Journals (Sweden)

    Bernardo Bucher B. Barbosa

    2015-07-01

    Full Text Available Este trabalho visa estudar maneiras de se explorar a Interação Humano Computador, usando Visão Computacional. A idéia tem como objetivo um esforço para tornar o computador mais interativo com o usuário, sem a necessidade da compra de um hardware ou acessório específico para tal. O produto final deste trabalho em desenvolvimento é um software que contempla esta funcionalidade, tornando o computador mais interativo.

  20. Interação do idoso na escola de samba

    Directory of Open Access Journals (Sweden)

    Alessandra Márcia Castro Soares

    2011-03-01

    Full Text Available É imprescindível que a pessoa idosa, em vez de ficar solitária ou cada vez mais sedentária, ligue-se a atividades que possam trazer a ela melhoria na sua qualidade de vida. A participação do idoso em uma escola de samba, por exemplo, pode trazer-lhe benefícios de várias ordens, ao proporcionar uma melhor comunicação em sua expressão verbal e não verbal e uma grande satisfação em conviver com outras pessoas no mesmo grupo. Esta pesquisa teve como objetivo conhecer os sentimentos de idosos participantes de atividades dentro de uma escola de samba, avaliando-se a interação com outras pessoas e os efeitos sobre a qualidade de vida e o bem-estar. Participaram do estudo 34 sujeitos com idade de 60 anos ou mais, em um estudo quali-quantitativo e descritivo. A análise dos dados revelou que a interação do idoso com os demais participantes da escola de samba oferece benefícios psicossociais de auto-estima, auto-cuidado, saúde, contribuindo significativamente para a melhoria da qualidade de vida à pessoa idosa.

  1. Análise de interações familiares: um estudo de caso

    Directory of Open Access Journals (Sweden)

    Ana Rita Coutinho Xavier Naves

    Full Text Available Este trabalho analisou as interações de uma família, em um contexto de laboratório, a partir de entrevistas com os pais e da participação dos membros familiares em tarefas análogas às do cotidiano - Lanche, Cartaz, Brincadeira e Organização da Sala, em um delineamento ABACA. A condição A correspondeu à linha de base. A variável independente foi um texto escrito, o qual descrevia comportamentos de uma família tradicional (Condição B e de uma família contemporânea (Condição C. A partir da categorização dos comportamentos dos participantes e do registro de intervalo parcial, observou-se que as interações familiares diferiram mais entre as tarefas, independentemente da condição, do que entre linha de base e condição experimental. Os resultados indicaram que os comportamentos dos participantes se assemelharam àqueles observados no ambiente natural.

  2. Interações mutualísticas entre formigas e plantas

    Directory of Open Access Journals (Sweden)

    Wesley Dáttilo

    2009-08-01

    Full Text Available O mutualismo entre formigas e plantas está entre as interações mais bem estudadas. Seu estudo vem sugerindo e testando hipóteses ecológicas e evolucionárias aplicáveis a muitas outras formas de mutualismo. A cada ano relações mais especializadas entre formigas e plantas são descobertas. Entre estas relações podemos mencionar a obtenção de abrigo e/ou alimento pela formiga, que implica no fornecimento de proteção a planta contra herbivoria, dispersão de suas sementes e, até, polinização de suas flores. Nesta revisão são discutidos os tipos de interações mutualísticas entre formigas e plantas existentes.Mutualistics interactions between ants and plantsAbstract. The mutualism between ant and plants is the one of the more studied ecological relationships, and these studies have been suggesting and testing ecological and evolutionary hypothesis that can be applied to many other kinds of mutualism. Each year more specialized mutualism relationships between ants and plants are discovered. Among these relations we can mention the obtaining of shelter and / or food by the ant, witch implies in providing plant protection against herbivory, dispersal of seeds and even pollination of their flowers. In this review are discussed mutualistics types of interactions between ants and existing plants.

  3. Tratamento farmacológico e interações medicamentosas em pacientes com aneurisma da aorta abdominal

    Directory of Open Access Journals (Sweden)

    Heverton Alves Peres

    2015-01-01

    Full Text Available O aneurisma da aorta abdominal (AAA ocorre quando há uma dilatação anormal e irreversível da artéria superior a 50% do calibre esperado para o vaso e associa-se a uma mortalidade de 80 a 90%. O controle dos principais fatores de risco como a hipertensão, dislipidemia, tabagismo e doenças trombóticas em aneurismas pequenos é feito por vários medicamentos que evitam o desenvolvimento e ruptura do aneurisma, no entanto, o uso destes associados a outros medicamentos pode desencadear interações medicamentosas relevantes sendo crucial o conhecimento sobre estas. Atualmente, há poucos dados na literatura sobre o tratamento farmacológico e interações medicamentosas em pacientes com AAA, sendo o objetivo desta revisão, descrever a farmacoterapia e interações medicamentosas em pacientes com AAA.

  4. MAGNET

    CERN Multimedia

    by B. Curé

    2011-01-01

    The magnet operation was very satisfactory till the technical stop at the end of the year 2010. The field was ramped down on 5th December 2010, following the successful regeneration test of the turbine filters at full field on 3rd December 2010. This will limit in the future the quantity of magnet cycles, as it is no longer necessary to ramp down the magnet for this type of intervention. This is made possible by the use of the spare liquid Helium volume to cool the magnet while turbines 1 and 2 are stopped, leaving only the third turbine in operation. This obviously requires full availability of the operators to supervise the operation, as it is not automated. The cryogenics was stopped on 6th December 2010 and the magnet was left without cooling until 18th January 2011, when the cryoplant operation resumed. The magnet temperature reached 93 K. The maintenance of the vacuum pumping was done immediately after the magnet stop, when the magnet was still at very low temperature. Only the vacuum pumping of the ma...

  5. Comparison of biochemical cartilage imaging techniques at 3 T MRI.

    Science.gov (United States)

    Rehnitz, C; Kupfer, J; Streich, N A; Burkholder, I; Schmitt, B; Lauer, L; Kauczor, H-U; Weber, M-A

    2014-10-01

    To prospectively compare chemical-exchange saturation-transfer (CEST) with delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) and T2 mapping to assess the biochemical cartilage properties of the knee. Sixty-nine subjects were prospectively included (median age, 42 years; male/female = 32/37) in three cohorts: 10 healthy volunteers, 40 patients with clinically suspected cartilage lesions, and 19 patients about 1 year after microfracture therapy. T2 mapping, dGEMRIC, and CEST were performed at a 3 T MRI unit using a 15-channel knee coil. Parameter maps were evaluated using region-of-interest analysis of healthy cartilage, areas of chondromalacia and repair tissue. Differentiation of damaged from healthy cartilage was assessed using receiver-operating characteristic (ROC) analysis. Chondromalacia grade 2-3 had significantly higher CEST values (P = 0.001), lower dGEMRIC (T1-) values (P < 0.001) and higher T2 values (P < 0.001) when compared to the normal appearing cartilage. dGEMRIC and T2 mapping correlated moderately negative (Spearman coefficient r = -0.56, P = 0.0018) and T2 mapping and CEST moderately positive (r = 0.5, P = 0.007), while dGEMRIC and CEST did not significantly correlate (r = -0.311, P = 0.07). The repair tissue revealed lower dGEMRIC values (P < 0.001) and higher CEST values (P < 0.001) with a significant negative correlation (r = -0.589, P = 0.01), whereas T2 values were not different (P = 0.54). In healthy volunteers' cartilage, CEST and dGEMRIC showed moderate positive correlation (r = 0.56), however not reaching significance (P = 0.09). ROC-analysis demonstrated non-significant differences of T2 mapping vs CEST (P = 0.14), CEST vs dGEMRIC (P = 0.89), and T2 mapping vs dGEMRIC (P = 0.12). CEST is able to detect normal and damaged cartilage and is non-inferior in distinguishing both when compared to dGEMRIC and T2 mapping. Copyright © 2014 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

  6. 26 CFR 1.132-3T - Qualified employee discount-1985 through 1988 (temporary).

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Qualified employee discount-1985 through 1988 (temporary). 1.132-3T Section 1.132-3T Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY....132-3T Qualified employee discount—1985 through 1988 (temporary). (a) In general—(1) Definition....

  7. 26 CFR 1.444-3T - Manner and time of making section 444 election (temporary).

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 6 2010-04-01 2010-04-01 false Manner and time of making section 444 election (temporary). 1.444-3T Section 1.444-3T Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Accounting Periods § 1.444-3T Manner and time of...

  8. Surto psicótico pela possível interação medicamentosa de sibutramina com finasterida

    OpenAIRE

    Douglas Dogol Sucar; Everton Botelho Sougey; José Brandão Neto

    2002-01-01

    É descrito um caso de interação medicamentosa em um paciente de 30 anos, que fazia com sucesso um tratamento antiobesidade com sibutramina. Esse medicamento é um saciogênico que inibe a recaptação da serotonina e da noradrenalina. Logo após a introdução da finasterida para tratamento da alopécia androgênica, o paciente passou a apresentar surto psicótico. Esse medicamento é um potente inibidor da 5-alfa-redutase do tipo II. A interação medicamentosa demonstra-se por meio de um rigoroso acompa...

  9. Eventos adversos por interações medicamentosas potenciais em unidade de terapia intensiva de um hospital de ensino

    OpenAIRE

    Mariana Macedo Alvim; Lidiane Ayres da Silva; Isabel Cristina Gonçalves Leite; Marcelo Silva Silvério

    2015-01-01

    RESUMO Objetivo: Avaliar a existência de interações medicamentosas potenciais na unidade de terapia intensiva de um hospital, com foco nos antimicrobianos. Métodos: Estudo transversal, que analisou prescrições eletrônicas de pacientes da unidade de terapia intensiva de um hospital de ensino, avaliando potenciais interações medicamentosas relacionadas aos antimicrobianos, entre 1º de janeiro e 31 de março de 2014. O consumo dos antimicrobianos foi expresso em dose diária definida por 100 pac...

  10. High-resolution 3-T MR neurography of peroneal neuropathy

    Energy Technology Data Exchange (ETDEWEB)

    Chhabra, Avneesh; Faridian-Aragh, Neda; Chalian, Majid; Soldatos, Theodoros; Thawait, Shrey K. [Johns Hopkins Hospital, The Russell H. Morgan Department of Radiology and Radiological Science, Baltimore, MD (United States); Williams, Eric H. [Johns Hopkins Hospital, Department of Plastic Surgery, Baltimore, MD (United States); Dellon Institute for Peripheral Nerve Surgery, Baltimore, MD (United States); Andreisek, Gustav [University Hospital Zurich, Institute for Diagnostic Radiology, Department of Medical Radiology, Zurich (Switzerland)

    2012-03-15

    The common peroneal nerve (CPN), a major terminal branch of the sciatic nerve, can be subject to a variety of pathologies, which may affect the nerve at any level from the lumbar plexus to its distal branches. Although the diagnosis of peripheral neuropathy is traditionally based on a patient's clinical findings and electrodiagnostic tests, magnetic resonance neurography (MRN) is gaining an increasing role in the definition of the type, site, and extent of peripheral nerve disorders. Current high-field MR scanners enable high-resolution and excellent soft-tissue contrast imaging of peripheral nerves. In the lower extremities, MR neurography has been employed in the demonstration of the anatomy and pathology of the CPN, as well as in the detection of associated secondary muscle denervation changes. This article reviews the normal appearance of the CPN as well as typical pathologies and abnormal findings at 3.0-T MR neurography of the lower extremity. (orig.)

  11. Lingüística de interações moleculares

    Directory of Open Access Journals (Sweden)

    Romeu Cardoso Guimarães

    1991-12-01

    Full Text Available As moléculas biológicas mais interessantes são longos polímeros. Em analogia com a linguagem humana alfabética, estes podem ser chamados de textos, e analisados, quanto à estrutura primária, como sequência de letras (monômeros; como nucleotídeos, aminoácidos, etc. ou de palavras (códigos de oligômeros, de até 5-6 letras. Considera-se que o estudo das palavras, em abordagem de tipo lingüístico, possa contribuir para o entendimento das interações (comunicações moleculares. As linguagens e dialetos, moleculares e humanos, são contrastados. A linguagem molecular se distingue peculiarmente da humana, por exemplo, por utilizar forma tridimensional, dinâmica temporal, ausência de espaçamento e pontuação, e sobreposição de significados. Apresenta-se um método matemático para descoberta, de palavras em textos. A palavra AAA (trinca de adeninas foi estudada na evolução do RNA ribossômico 5S. Observou-se que esta palavra é mais freqüente em organismos menos complexos e menos freqüente nos mais complexos, das linhagens de fungos, plantas e vertebrados. Nas duas últimas, reduziu-se também o grau de variabilidade gênica. Pelo contrário, grau moderado de freqüência da palavra persistiu em toda a linhagem dos invertebrados, com manutenção paralela de alto nível de variabilidade gênica. Nas mitocôndrias, plastídeos e micoplasmas, a freqüência da palavra AAA foi aumentada, de acordo com sua necessidade de interações com maior amplitude de variação. Esses comportamentos indicam que a palavra monótona AAA permite ambigüidade de interações. Com a evolução da complexidade orgânica e da maior especificidade molecular, as palavras ambíguas foram progressivamente evitadas.

  12. Interação medicamentosa de venlafaxina com captopril Drug interaction of velanfaxine with captopril

    Directory of Open Access Journals (Sweden)

    Douglas D Sucar

    2000-09-01

    Full Text Available O autor descreve um caso de interação medicamentosa, em uma senhora de 53 anos de idade, com diagnóstico de depressão e de hipertensão arterial. Com níveis pressóricos estáveis, em conseqüência de um regime dietético e de uso do captopril -anti-hipertensivo inibidor da enzima de conversão da angiotensina -, passou a apresentar constantes descompensações do seu quadro clínico, com elevações da tensão arterial (TA, logo após a introdução da venlafaxina no seu esquema terapêutico. Esse medicamento é um potente antidepressivo de última geração, que atua no sistema nervoso central (SNC, inibindo a recaptação de serotonina e noradrenalina. Demonstra a interação pelo monitoramento da TA e aplicação do instrumento de Naranjo. Descreve as condições clínicas gerais da paciente e sua evolução, e discute os mecanismos prováveis que conduziram a interação pela hipótese que envolve o aumento de noradrenalina nos terminais sinápticos, o sistema renina-angiotensina e a bradicinina, concluindo que a venlafaxina agiu como antagonista, de modo indireto, sobre os efeitos hipotensores do captopril.This is a case report of drug interaction in a 53 year-old woman diagnosed with depression and arterial hypertension. As a result of a low-salt diet and the use of the captopril (an antihypertensive that inhibites the angiotensine conversion enzyme, her pressoric levels had been stable till venlafaxine was introduced in her therapeutic regime. By then she started to show an unstableclinical condition, with elevations of her arterial blood pressure (ABP. Venlafaxine is a potent last generation antidepressant drug, acting in the central nervous system (CNS by inhibiting the reuptake of serotonine and noradrenaline. The drug interaction is demonstrated by monitoring the ABP and using the Naranjo's tool.The patient's general clinical conditions and herprogress are presented, and the hypothetical mechanisms to the interaction, such as

  13. MAGNET

    CERN Multimedia

    B. Curé

    2012-01-01

      The magnet was energised at the beginning of March 2012 at a low current to check all the MSS safety chains. Then the magnet was ramped up to 3.8 T on 6 March 2012. Unfortunately two days later an unintentional switch OFF of the power converter caused a slow dump. This was due to a misunderstanding of the CCC (CERN Control Centre) concerning the procedure to apply for the CMS converter control according to the beam-mode status at that time. Following this event, the third one since 2009, a discussion was initiated to define possible improvement, not only on software and procedures in the CCC, but also to evaluate the possibility to upgrade the CMS hardware to prevent such discharge from occurring because of incorrect procedure implementations. The magnet operation itself was smooth, and no power cuts took place. As a result, the number of magnetic cycles was reduced to the minimum, with only two full magnetic cycles from 0 T to 3.8 T. Nevertheless the magnet suffered four stops of the cryogeni...

  14. MAGNET

    CERN Multimedia

    Benoit Curé

    2010-01-01

    Operation of the magnet has gone quite smoothly during the first half of this year. The magnet has been at 4.5K for the full period since January. There was an unplanned short stop due to the CERN-wide power outage on May 28th, which caused a slow dump of the magnet. Since this occurred just before a planned technical stop of the LHC, during which access in the experimental cavern was authorized, it was decided to leave the magnet OFF until 2nd June, when magnet was ramped up again to 3.8T. The magnet system experienced a fault also resulting in a slow dump on April 14th. This was triggered by a thermostat on a filter choke in the 20kA DC power converter. The threshold of this thermostat is 65°C. However, no variation in the water-cooling flow rate or temperature was observed. Vibration may have been the root cause of the fault. All the thermostats have been checked, together with the cables, connectors and the read out card. The tightening of the inductance fixations has also been checked. More tem...

  15. MAGNET

    CERN Multimedia

    B. Curé

    2012-01-01

      Following the unexpected magnet stops last August due to sequences of unfortunate events on the services and cryogenics [see CMS internal report], a few more events and initiatives again disrupted the magnet operation. All the magnet parameters stayed at their nominal values during this period without any fault or alarm on the magnet control and safety systems. The magnet was stopped for the September technical stop to allow interventions in the experimental cavern on the detector services. On 1 October, to prepare the transfer of the liquid nitrogen tank on its new location, several control cables had to be removed. One cable was cut mistakenly, causing a digital input card to switch off, resulting in a cold-box (CB) stop. This tank is used for the pre-cooling of the magnet from room temperature down to 80 K, and for this reason it is controlled through the cryogenics control system. Since the connection of the CB was only allowed for a field below 2 T to avoid the risk of triggering a fast d...

  16. Diffusion tensor imaging applications in multiple sclerosis patients using 3T magnetic resonance: a preliminary study

    Energy Technology Data Exchange (ETDEWEB)

    Testaverde, Lorenzo; Caporali, Laura [University ' ' Sapienza' ' of Rome, Department of Radiological Sciences, Rome (Italy); Venditti, Eugenio; Grillea, Giovanni [U.O.C. Neuroradiologia, I.R.C.C.S. ' ' Neuromed' ' , Pozzilli (Italy); Colonnese, Claudio [University ' ' Sapienza' ' of Rome, Department of Radiological Sciences, Rome (Italy); U.O.C. Neuroradiologia, I.R.C.C.S. ' ' Neuromed' ' , Pozzilli (Italy)

    2012-05-15

    This study evaluated patients with multiple sclerosis using diffusion tensor imaging (DTI) to obtain fractional anisotropy (FA) and mean diffusivity (MD) values. We investigated the possible statistically significant variation of MD and FA in different MS patients, compared simultaneously, putting in comparison their normal appearing white matter (NAWM) and white matter affected by disease (plaques), both during activity and in remission, with normal white matter (NWM) of control subjects. Statistical analysis using Levene's test for comparison of variances revealed significant (P < 0.05) differences between FA values of the NWM of the controls and those of NAWM and active or inactive lesions, of the patients in the study. However, the differences between MD values of the NWM of the controls and those of NAWM and active or inactive lesions of the patients in the study were judged not significant (P > 0.05). Imaging of MS using MRI techniques is constantly searching for reproducible quantitative parameter. This study shows how these parameters can be identified in the MD and FA values, and thus suggests the implementation of MRI routine protocols for diagnosing MS with the DTI analysis, since it can provide valuable information otherwise unobtainable. (orig.)

  17. Interações medicamentosas: fundamentos para a pratica clínica da enfermagem

    OpenAIRE

    2001-01-01

    O fenômeno das interações medicamentosas constitui na atualidade um dos temas mais importantes da farmacologia, para a prática clínica dos profissionais da saúde. O uso concomitante de vários medicamentos, enquanto estratégia terapêutica, e o crescente número destes agentes no mercado são alguns dos fatores que contribuem para ampliar os efeitos benéficos da terapia, mas que também possibilitam a interferência mútua de ações farmacológicas podendo resultar em alterações dos efeitos desejados....

  18. Interação dos anestesicos locais benzocaina, lidocaina e tetracaina com membranas modelo

    OpenAIRE

    Luciana de Matos Alves Pinto

    1998-01-01

    Resumo: Dentre as teorias sobre o mecanismo de ação de anestésicos locais (AL) podemos destacar as que tentam explicar efeitos diretos sobre a proteína canal de sódio, o aquelas que enfocam os efeitos da interação dos anestésicos com a fase lipídica membranar. Sabe-se que há uma correlação direta entre a hidrofobicidade da molécula anestésica (medida pelo coeficiente de partição) e a sua potência clínica. Sendo assim, é natural pensar que qualquer que seja o mecanismo de ação dos AL ele deve ...

  19. Interações públicas, censura privada: o caso do Facebook

    Directory of Open Access Journals (Sweden)

    Sergio Amadeu da Silveira

    Full Text Available Resumo O Facebook é apresentado como uma plataforma de relacionamento social on-line transnacional em que ocorrem debates e interações públicas. O texto traz um levantamento sobre o controle privado das expressões culturais e políticas realizadas pelos gestores da plataforma, que pode ser definido como censura privada. Casos de remoção de conteúdo são apresentados, e são expostas as políticas de controle do Facebook. O texto mostra ainda que as regras do Facebook trazem fortes componentes discricionários e uma estética que pode ser apresentada como um tipo de biopolítica da sociedade informacional.

  20. Interação adulto-criança - uma - perspetiva de qualidade na aprendizagem

    OpenAIRE

    Jorge, Anabela Ferreira

    2013-01-01

    A presente investigação teve como objetivo principal investigar a interação adulto – criança: tentando compreender as dimensões do estilo do educador. Neste sentido, tornou-se pertinente fazer uma abordagem da educação pré-escolar explicitando a educação de Infância no seu contexto histórico. A educação pré-escolar passou por várias momentos, resultantes do contexto social, político e económico do país, até ser reconhecida e valorizada como sendo uma etapa imprescindível no que...

  1. Rádio escolar: uma ferramenta de interação sociodiscursiva

    Directory of Open Access Journals (Sweden)

    Marcos Baltar

    2008-01-01

    Full Text Available Este trabalho discute a implementação de Rádios Escolares em escolas públicas da região do entorno da Universidade de Caxias do Sul como ferramentas de interação sociodiscursiva, visando estimular o protagonismo social das comunidades escolares envolvidas, bem como oferecer novos caminhos para a promoção do desenvolvimento e aprendizagem de crianças, jovens e adultos.In this work we present the experience of implementing radio stations in state schools in the city of Caxias do Sul - Brazil, as a tool for teaching and learning. The main goal of radios is to create the space for sociodiscursive interaction in order to stimulate the social protagonism in scholar communities involved in the project. The radio also showed as a different tool to enhance children, teenagers and adults learning.

  2. Diabetes em idosos: uso de medicamentos e risco de interação medicamentosa

    Directory of Open Access Journals (Sweden)

    Maria Aparecida Medeiros Barros do Prado

    Full Text Available Resumo Caracterizar o perfil sociodemográfico e de saúde dos idosos segundo diabetes referido, avaliar o conhecimento e a prática quanto às opções de tratamento, bem como descrever o uso de medicamentos e potenciais riscos de interação medicamentosa (IM neste subgrupo. Estudo transversal com 1.517 idosos de Campinas em 2008. Estimaram-se as prevalências de diabetes e verificaram-se as associações pelo teste de Rao-Scott. As potenciais IM foram avaliadas pela base de dados Micromedex®. A prevalência de diabetes referida pelos idosos foi de 21,7% sem diferença significativa entre os sexos. Verificou-se maior percentual de idosos diabéticos com 70 anos ou mais, com menor escolaridade, renda familiar per capita inferior a 1 salário mínimo e que não realizavam atividade ocupacional. O número médio de medicamentos foi de 3,9 nos 3 dias anteriores. Identificaram-se 413 possíveis interações, sendo que 53,1%, 7,8% e 7,2% dos idosos apresentaram risco de IM moderadas, menores e graves, respectivamente. Ressalta-se a importância da adoção de dieta saudável e prática de atividade física para redução do peso, controle da doença e de complicações. Evidencia-se a necessidade de atenção ao risco potencial das IM e o uso de medicamentos inapropriados ao idoso.

  3. Neurografía por resonancia magnética en 3T: experiencia preliminar 3T MRI neurography: preliminary experience

    Directory of Open Access Journals (Sweden)

    Martín Aguilar

    2012-12-01

    Asymmetry and Least-squares estimation sequence in the evaluation of peripheral nerves, brachial plexus and lumbar plexus, for the diagnosis of compression or entrapment neuropathies, non-entrapment neuropathies, and the underlying conditions. The IDEAL sequence provides 4 types of images from a single acquisition, allowing uniform fat or water suppression and in phase/out of phase images of water, fat or a combination of both. Materials and Methods. This is a retrospective study, from January 2011 to June 2011. Eleven neurographies were performed on 3T MRI (HDX 3T, GE Healthcare, USA, with 8-channel phased array coils on sagittal and coronal planes, with 1.2-0 mm slices with no gap, axial 3D spoiled gradient recalled (SPGR T , with 1-0-mm slice thickness with and without gadolinium injection and variable field of view (FOV according to the nerve or plexus to explore. Results. We found 2 schwannomas (brachial plexus and sciatic nerve, 1 neuritis (compression to median nerve, 2 cases of multiple neurofibromas (lumbosacral plexus, sciatic nerve, brachial plexus, 3 traumatic neuromas (peroneal nerve and 1 pseudomeningocele avulsion (brachial plexus, and 2 with no structural alterations (lumbosacral plexus and peroneal nerve. Conclusion. In this preliminary experience, the use of highresolution sequences in magnetic resonance imaging neurography studies provided excellent signal homogeneity, improving the recognition of the nerve structure and signal, the identification of anatomical variations, and causes of neuropathy, as well as the characterization of denervation changes of the affected muscle groups.

  4. MAGNET

    CERN Multimedia

    B. Curé

    2012-01-01

      The magnet and its sub-systems were stopped at the beginning of the winter shutdown on 8th December 2011. The magnet was left without cooling during the cryogenics maintenance until 17th January 2012, when the cryoplant operation resumed. The magnet temperature reached 93 K. The vacuum pumping was maintained during this period. During this shutdown, the yearly maintenance was performed on the cryogenics, the vacuum pumps, the magnet control and safety systems, and the power converter and discharge lines. Several preventive actions led to the replacement of the electrovalve command coils, and the 20A DC power supplies of the magnet control system. The filters were cleaned on the demineralised water circuits. The oil of the diffusion pumps was changed. On the cryogenics, warm nitrogen at 343 K was circulated in the cold box to regenerate the filters and the heat exchangers. The coalescing filters have been replaced at the inlet of both the turbines and the lubricant trapping unit. The active cha...

  5. MAGNET

    CERN Multimedia

    Benoit Curé

    2010-01-01

    The magnet was successfully operated at the end of the year 2009 despite some technical problems on the cryogenics. The magnet was ramped up to 3.8 T at the end of November until December 16th when the shutdown started. The magnet operation met a few unexpected stops. The field was reduced to 3.5 T for about 5 hours on December 3rd due to a faulty pressure sensor on the helium compressor. The following day the CERN CCC stopped unintentionally the power converters of the LHC and the experiments, triggering a ramp down that was stopped at 2.7 T. The magnet was back at 3.8 T about 6 hours after CCC sent the CERN-wide command. Three days later, a slow dump was triggered due to a stop of the pump feeding the power converter water-cooling circuit, during an intervention on the water-cooling plant done after several disturbances on the electrical distribution network. The magnet was back at 3.8 T in the evening the same day. On December 10th a break occurred in one turbine of the cold box producing the liquid ...

  6. MAGNET

    CERN Multimedia

    B. Curé

    2013-01-01

      The magnet was operated without any problem until the end of the LHC run in February 2013, apart from a CERN-wide power glitch on 10 January 2013 that affected the CMS refrigerator, causing a ramp down to 2 T in order to reconnect the coldbox. Another CERN-wide power glitch on 15 January 2013 didn’t affect the magnet subsystems, the cryoplant or the power converter. At the end of the magnet run, the reconnection of the coldbox at 2.5 T was tested. The process will be updated, in particular the parameters of some PID valve controllers. The helium flow of the current leads was reduced but only for a few seconds. The exercise will be repeated with the revised parameters to validate the automatic reconnection process of the coldbox. During LS1, the water-cooling services will be reduced and many interventions are planned on the electrical services. Therefore, the magnet cryogenics and subsystems will be stopped for several months, and the magnet cannot be kept cold. In order to avoid unc...

  7. MAGNET

    CERN Multimedia

    B. Curé

    2011-01-01

    The CMS magnet has been running steadily and smoothly since the summer, with no detected flaw. The magnet instrumentation is entirely operational and all the parameters are at their nominal values. Three power cuts on the electrical network affected the magnet run in the past five months, with no impact on the data-taking as the accelerator was also affected at the same time. On 22nd June, a thunderstorm caused a power glitch on the service electrical network. The primary water cooling at Point 5 was stopped. Despite a quick restart of the water cooling, the inlet temperature of the demineralised water on the busbar cooling circuit increased by 5 °C, up to 23.3 °C. It was kept below the threshold of 27 °C by switching off other cooling circuits to avoid the trigger of a slow dump of the magnet. The cold box of the cryogenics also stopped. Part of the spare liquid helium volume was used to maintain the cooling of the magnet at 4.5 K. The operators of the cryogenics quickly restarted ...

  8. Prediction of prostate cancer extracapsular extension with high spatial resolution dynamic contrast-enhanced 3-T MRI

    Energy Technology Data Exchange (ETDEWEB)

    Bloch, B.N. [Beth Israel Deaconess Medical Center and Harvard Medical School, Department of Radiology, Boston, MA (United States); Boston Medical Center and Boston University, Department of Radiology, Boston, MA (United States); Genega, Elizabeth M. [Beth Israel Deaconess Medical Center and Harvard Medical School, Department of Pathology, Boston, MA (United States); Costa, Daniel N.; Pedrosa, Ivan; Rofsky, Neil M. [Beth Israel Deaconess Medical Center and Harvard Medical School, Department of Radiology, Boston, MA (United States); University of Texas Southwestern Medical Center, Department of Radiology, Dallas, TX (United States); Smith, Martin P.; Kressel, Herbert Y. [Beth Israel Deaconess Medical Center and Harvard Medical School, Department of Radiology, Boston, MA (United States); Ngo, Long [Beth Israel Deaconess Medical Center and Harvard Medical School, Department of General Medicine, Boston, MA (United States); Sanda, Martin G.; DeWolf, William C. [Beth Israel Deaconess Medical Center and Harvard Medical School, Department of Division of Urology, Boston, MA (United States)

    2012-10-15

    To assess the value of dynamic contrast-enhanced (DCE) combined with T2-weighted (T2W) endorectal coil (ERC) magnetic resonance imaging (MRI) at 3 T for determining extracapsular extension (ECE) of prostate cancer. In this IRB-approved study, ERC 3-T MRI of the prostate was performed in 108 patients before radical prostatectomy. T2W fast spin-echo and DCE 3D gradient echo images were acquired. The interpretations of readers with varied experience were analysed. MRI-based staging results were compared with radical prostatectomy histology. Descriptive statistics were generated for prediction of ECE and staging accuracies were determined by the area under the receiver-operating characteristic curve. The overall sensitivity, specificity, positive predictive value and negative predictive value for ECE were 75 %, 92 %, 79 % and 91 %, respectively. Diagnostic accuracy for staging was 86 %, 80 % and 91 % for all readers, experienced and less experienced readers, respectively. ERC 3-T MRI of the prostate combining DCE and T2W imaging is an accurate pretherapeutic staging tool for assessment of ECE in clinical practice across varying levels of reader experience. (orig.)

  9. Detection of small pulmonary nodules in high-field MR at 3 T: evaluation of different pulse sequences using porcine lung explants

    Energy Technology Data Exchange (ETDEWEB)

    Regier, M.; Kaul, M.G.; Ittrich, H.; Bansmann, P.M.; Kemper, J.; Nolte-Ernsting, C.; Adam, G. [University Hospital of Hamburg, Center for Diagnostic Imaging and Image Guided Therapy, Department of Diagnostic and Interventional Radiology, Hamburg (Germany); Kandel, S.; Hoffmann, B.; Heller, M.; Biederer, J. [University Hospital of Kiel, Department of Diagnostic Radiology, Kiel (Germany)

    2007-05-15

    To evaluate two MR imaging sequences for the detection of artificial pulmonary nodules inside porcine lung explants. 67 agarose nodules ranging 3-20 mm were injected into ten porcine lungs within a dedicated chest phantom. The signal on T1-weighted images and radiopacity were adjusted by adding 0.125 mmol/l Gd-DTPA and 1.5 g/l of iodine. A T1-weighted three-dimensional gradient-echo (T1-3D-GRE; TR/TE:3.3/1.1 ms, slice:8 mm, flip-angle:10 ) and a T2-weighted half-Fourier fast-spin echo sequence (T2-HF-FSE; TR/TE:2000/66 ms, slice:7 mm, flip-angle:90 ) were applied in axial orientation using a 3-T system (Intera, Philips Medical Systems, Best, The Netherlands), followed by CT (16 x 0.5 mm) as reference. Nodule sizes and locations were assessed by three blinded observers. In nodules of >10 mm, sensitivity was 100% using 3D-GRE-MRI and 94% using the HF-FSE sequence. For nodules 6-10 mm, the sensitivity of MRI was lower than with CT (3D-GRE:92%; T2-HF-FSE:83%). In lesions smaller than 5 mm, the sensitivity declined to 80% (3D-GRE) and 53% (HF-FSE). Small lesion diameters were overestimated with both sequences, particularly with HF-FSE. This study confirms the feasibility of 3 T-MRI for lung nodule detection. In lesions greater than 5 mm, the sensitivity of the 3D-GRE sequence approximated CT (>90%), while sensitivity and PPV with the HF-FSE sequence were slightly inferior. (orig.)

  10. MAGNETS

    Science.gov (United States)

    Hofacker, H.B.

    1958-09-23

    This patent relates to nmgnets used in a calutron and more particularly to means fur clamping an assembly of magnet coils and coil spacers into tightly assembled relation in a fluid-tight vessel. The magnet comprises windings made up of an assembly of alternate pan-cake type coils and spacers disposed in a fluid-tight vessel. At one end of the tank a plurality of clamping strips are held firmly against the assembly by adjustable bolts extending through the adjacent wall. The foregoing arrangement permits taking up any looseness which may develop in the assembly of coils and spacers.

  11. Lysophosphatidic acid receptor-5 negatively regulates cellular responses in mouse fibroblast 3T3 cells

    Energy Technology Data Exchange (ETDEWEB)

    Dong, Yan; Hirane, Miku; Araki, Mutsumi [Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502 (Japan); Fukushima, Nobuyuki [Division of Molecular Neurobiology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502 (Japan); Tsujiuchi, Toshifumi, E-mail: ttujiuch@life.kindai.ac.jp [Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502 (Japan)

    2014-04-04

    Highlights: • LPA{sub 5} inhibits the cell growth and motile activities of 3T3 cells. • LPA{sub 5} suppresses the cell motile activities stimulated by hydrogen peroxide in 3T3 cells. • Enhancement of LPA{sub 5} on the cell motile activities inhibited by LPA{sub 1} in 3T3 cells. • The expression and activation of Mmp-9 were inhibited by LPA{sub 5} in 3T3 cells. • LPA signaling via LPA{sub 5} acts as a negative regulator of cellular responses in 3T3 cells. - Abstract: Lysophosphatidic acid (LPA) signaling via G protein-coupled LPA receptors (LPA{sub 1}–LPA{sub 6}) mediates a variety of biological functions, including cell migration. Recently, we have reported that LPA{sub 1} inhibited the cell motile activities of mouse fibroblast 3T3 cells. In the present study, to evaluate a role of LPA{sub 5} in cellular responses, Lpar5 knockdown (3T3-L5) cells were generated from 3T3 cells. In cell proliferation assays, LPA markedly stimulated the cell proliferation activities of 3T3-L5 cells, compared with control cells. In cell motility assays with Cell Culture Inserts, the cell motile activities of 3T3-L5 cells were significantly higher than those of control cells. The activity levels of matrix metalloproteinases (MMPs) were measured by gelatin zymography. 3T3-L5 cells stimulated the activation of Mmp-2, correlating with the expression levels of Mmp-2 gene. Moreover, to assess the co-effects of LPA{sub 1} and LPA{sub 5} on cell motile activities, Lpar5 knockdown (3T3a1-L5) cells were also established from Lpar1 over-expressing (3T3a1) cells. 3T3a1-L5 cells increased the cell motile activities of 3T3a1 cells, while the cell motile activities of 3T3a1 cells were significantly lower than those of control cells. These results suggest that LPA{sub 5} may act as a negative regulator of cellular responses in mouse fibroblast 3T3 cells, similar to the case for LPA{sub 1}.

  12. MAGNET

    CERN Multimedia

    B. Curé

    2011-01-01

    The magnet ran smoothly in the last few months until a fast dump occurred on 9th May 2011. Fortunately, this occurred in the afternoon of the first day of the technical stop. The fast dump was due to a valve position controller that caused the sudden closure of a valve. This valve is used to regulate the helium flow on one of the two current leads, which electrically connects the coil at 4.5 K to the busbars at room temperature. With no helium flow on the lead, the voltage drop and the temperatures across the leads increase up to the defined thresholds, triggering a fast dump through the Magnet Safety System (MSS). The automatic reaction triggered by the MSS worked properly. The helium release was limited as the pressure rise was just at the limit of the safety valve opening pressure. The average temperature of the magnet reached 72 K. It took four days to recover the temperature and refill the helium volumes. The faulty valve controller was replaced by a spare one before the magnet ramp-up resumed....

  13. In vivo conductivity imaging of canine male pelvis using a 3T MREIT system

    Science.gov (United States)

    Kim, H. J.; Jeong, W. C.; Kim, Y. T.; Minhas, A. S.; Lee, T. H.; Lim, C. Y.; Park, H. M.; Seo, J. K.; Woo, E. J.

    2010-04-01

    The prostate is an imaging area of growing concern related with aging. Prostate cancer and benign prostatic hyperplasia are the most common diseases and significant cause of death for elderly men. Hence, the conductivity imaging of the male pelvis is a challenging task with a clinical significance. In this study, we performed in vivo MREIT imaging experiments of the canine male pelvis using a 3T MRI scanner. Adopting carbon-hydrogel electrodes and a multi-echo pulse sequence, we could inject as much as 10 mA current in a form of 51 ms pulse into the pelvis. Collecting magnetic flux density data inside the pelvis subject to multiple injection currents, we reconstructed cross-sectional conductivity images using a MREIT software package CoReHA. Scaled conductivity images of the prostate show a clear contrast between the central and peripheral zones which are related with prostate diseases including cancer and benign prostatic hyperplasia. In our future work, we will focus on prostate cancer model animal experiments.

  14. Subendocardial contractile impairment in chronic ischemic myocardium: assessment by strain analysis of 3T tagged CMR

    Directory of Open Access Journals (Sweden)

    Nagao Michinobu

    2012-02-01

    Full Text Available Abstract Background The purpose of this study was to quantify myocardial strain on the subendocardial and epicardial layers of the left ventricle (LV using tagged cardiovascular magnetic resonance (CMR and to investigate the transmural degree of contractile impairment in the chronic ischemic myocardium. Methods 3T tagged CMR was performed at rest in 12 patients with severe coronary artery disease who had been scheduled for coronary artery bypass grafting. Circumferential strain (C-strain at end-systole on subendocardial and epicardial layers was measured using the short-axis tagged images of the LV and available software (Intag; Osirix. The myocardial segment was divided into stenotic and non-stenotic segments by invasive coronary angiography, and ischemic and non-ischemic segments by stress myocardial perfusion scintigraphy. The difference in C-strain between the two groups was analyzed using the Mann-Whitney U-test. The diagnostic capability of C-strain was analyzed using receiver operating characteristics analysis. Results The absolute subendocardial C-strain was significantly lower for stenotic (-7.5 ± 12.6% than non-stenotic segment (-18.8 ± 10.2%, p Conclusions Analysis of tagged CMR can non-invasively demonstrate predominant impairment of subendocardial strain in the chronic ischemic myocardium at rest.

  15. Neural correlates of simple unimanual discrete and continuous movements: a functional imaging study at 3 T

    Energy Technology Data Exchange (ETDEWEB)

    Habas, Christophe; Cabanis, Emmanuel A. [Hopital des Quinze-Vingts, UPMC Paris 6, Service de NeuroImagerie, Paris (France)

    2008-04-15

    The cerebral and cerebellar network involved in unimanual continuous and discrete movements was studied in blood oxygenation level-dependent functional magnetic resonance imaging (fMRI) at 3 T. Seven healthy right-handed volunteers were scanned (1) while drawing a circle with the tip of the right index finger (continuous motor task), and (2) while drawing a triangle with the tip of the right index finger (discrete motor task). In both motor tasks, extensive activations were observed in the sensorimotor (M1/S1), parietal, prefrontal, insular, lateral occipital (LOC) and anterior cerebellar cortices. Subcortical activations within red, thalamic and lentiform nuclei were also detected. However, discrete movements were specifically followed by the recruitment of the left orbitofrontal cortex, right dentate nucleus and the second cerebellar homunculus (HVIII), and bilateral and stronger activation of the sensorimotor cortical areas, whereas continuous movements specifically activated the right prefrontal cortex and the lateral hemispherical part of the neocerebellum (crus 1). We confirm the findings of previous studies showing partly distinct neural networks involved in monitoring continuous and discrete movements, but we found new differential neural relays within the prefrontal, insular and neocerebellar cortices. (orig.)

  16. Monitoring fractional anisotropy in developing rabbit brain using MR diffusion tensor imaging at 3T

    Science.gov (United States)

    Jao, Jo-Chi; Yang, Yu-Ting; Hsiao, Chia-Chi; Chen, Po-Chou

    2016-03-01

    The aim of this study was to investigate the factional anisotropy (FA) in various regions of developing rabbit brain using magnetic resonance diffusion tensor imaging (MR DTI) at 3 T. A whole-body clinical MR imaging (MRI) scanner with a 15-channel high resolution knee coil was used. An echo-planar-imaging (EPI)-DTI pulse sequence was performed. Five 5 week-old New Zealand white (NZW) rabbits underwent MRI once per week for 24 weeks. After scanning, FA maps were obtained. ROIs (regions of interests) in the frontal lobe, parietal & temporal lobe, and occipital lobe were measured. FA changes with time were evaluated with a linear regression analysis. The results show that the FA values in all lobes of the brain increased linearly with age. The ranking of FA values was FA(frontal lobe) FA(occipital lobe). There was significant difference (p brain functions. The FA change rate could be a biomarker to monitor the brain development. Understanding the FA values of various lobes during development could provide helpful information to diagnosis the abnormal syndrome earlier and have a better treatment and prognosis. This study established a brain MR-DTI protocol for rabbits to investigate the brain anatomy during development using clinical MRI. This technique can be further applied to the pre-clinical diagnosis, treatment, prognosis and follow-up of brain lesions.

  17. Accuracy of 3 T versus 1.5 T breast MRI for pre-operative assessment of extent of disease in newly diagnosed DCIS

    Energy Technology Data Exchange (ETDEWEB)

    Rahbar, Habib, E-mail: hrahbar@uw.edu; DeMartini, Wendy B.; Lee, Amie Y.; Partridge, Savannah C.; Peacock, Sue; Lehman, Constance D.

    2015-04-15

    Highlights: •We compared sizes of known ductal carcinoma in situ (DCIS) on pre-operative breast MRI at 3 T and 1.5 T with final pathology sizes. •DCIS sizes on 3 T MRI correlated better with pathologic sizes than 1.5 T MRI. •Imaging features of DCIS, including morphology and kinetics, were similar at 3 T and 1.5 T MRI. -- Abstract: Objectives: While 3 T breast magnetic resonance imaging has increased in use over the past decade, there is little data comparing its use for assessing ductal carcinoma in situ (DCIS) versus 1.5 T. We sought to compare the accuracies of DCIS extent of disease measures on pre-operative 3 T versus 1.5 T MRI. Methods: This institutional review board-approved prospective study included 20 patients with ductal carcinoma in situ diagnosed by core needle biopsy (CNB) who underwent pre-operative breast MRI at both 3 T (resolution = 0.5 mm × 0.5 mm × 1.3 mm) and 1.5 T (0.85 mm × 0.85 mm × 1.6 mm). All patients provided informed consent, and the study was HIPPA compliant. Lesion sizes and imaging characteristics (morphologic and kinetic enhancement) were recorded for the 3 T and 1.5 T examinations. Lesion size measures at both field strengths were correlated to final pathology, and imaging characteristics also were compared. Results: Of the initial cohort of 20 patients with CNB-diagnosed DCIS, 19 underwent definitive surgery. Median DCIS sizes of these 19 patients were 6 mm (range: 0–67 mm) on 3 T, 13 mm (0–60 mm) on 1.5 T, and 6 mm (0–55 mm) on surgical pathology. Size correlation between MRI and pathology was higher for 3 T (Spearman's ρ = 0.66, p = 0.002) than 1.5 T (ρ = 0.36, p = 0.13). In 10 women in which a residual area of suspicious enhancement was identified on both field strengths, there was agreement of morphologic description (NME vs. mass) in nine, and no significant difference in dynamic contrast enhanced kinetics at 3 T compared to 1.5 T. Conclusions: Pre-operative breast MRI at 3 T provided higher

  18. MAGNET

    CERN Multimedia

    B. Curé

    2013-01-01

    The magnet is fully stopped and at room temperature. The maintenance works and consolidation activities on the magnet sub-systems are progressing. To consolidate the cryogenic installation, two redundant helium compressors will be installed as ‘hot spares’, to avoid the risk of a magnet downtime in case of a major failure of a compressor unit during operation. The screw compressors, their motors, the mechanical couplings and the concrete blocks are already available and stored at P5. The metallic structure used to access the existing compressors in SH5 will be modified to allow the installation of the two redundant ones. The plan is to finish the installation and commissioning of the hot spare compressors before the summer 2014. In the meantime, a bypass on the high-pressure helium piping will be installed for the connection of a helium drier unit later during the Long Shutdown 1, keeping this installation out of the schedule critical path. A proposal is now being prepared for the con...

  19. MAGNET

    CERN Multimedia

    B. Curé

    MAGNET During the winter shutdown, the magnet subsystems went through a full maintenance. The magnet was successfully warmed up to room temperature beginning of December 2008. The vacuum was broken later on by injecting nitrogen at a pressure just above one atmosphere inside the vacuum tank. This was necessary both to prevent any accidental humidity ingress, and to allow for a modification of the vacuum gauges on the vacuum tank and maintenance of the diffusion pumps. The vacuum gauges had to be changed, because of erratic variations on the measurements, causing spurious alarms. The new type of vacuum gauges has been used in similar conditions on the other LHC experiments and without problems. They are shielded against the stray field. The lubricants of the primary and diffusion pumps have been changed. Several minor modifications were also carried out on the equipment in the service cavern, with the aim to ease the maintenance and to allow possible intervention during operation. Spare sensors have been bough...

  20. MAGNET

    CERN Multimedia

    Benoit Curé

    2010-01-01

    The magnet worked very well at 3.8 T as expected, despite a technical issue that manifested twice in the cryogenics since June. All the other magnet sub-systems worked without flaw. The issue in the cryogenics was with the cold box: it could be observed that the cold box was getting progressively blocked, due to some residual humidity and air accumulating in the first thermal exchanger and in the adsorber at 65 K. This was later confirmed by the analysis during the regeneration phases. An increase in the temperature difference between the helium inlet and outlet across the heat exchanger and a pressure drop increase on the filter of the adsorber were observed. The consequence was a reduction of the helium flow, first compensated by the automatic opening of the regulation valves. But once they were fully opened, the flow and refrigeration power reduced as a consequence. In such a situation, the liquid helium level in the helium Dewar decreased, eventually causing a ramp down of the magnet current and a field...

  1. MAGNET

    CERN Multimedia

    Benoit Curé.

    The magnet operation restarted end of June this year. Quick routine checks of the magnet sub-systems were performed at low current before starting the ramps up to higher field. It appeared clearly that the end of the field ramp down to zero was too long to be compatible with the detector commissioning and operations plans. It was decided to perform an upgrade to keep the ramp down from 3.8T to zero within 4 hours. On July 10th, when a field of 1.5T was reached, small movements were observed in the forward region support table and it was decided to fix this problem before going to higher field. At the end of July the ramps could be resumed. On July 28th, the field was at 3.8T and the summer CRAFT exercise could start. This run in August went smoothly until a general CERN wide power cut took place on August 3rd, due to an insulation fault on the high voltage network outside point 5. It affected the magnet powering electrical circuit, as it caused the opening of the main circuit breakers, resulting in a fast du...

  2. Multiparametric 3T MRI in the evaluation of intraglandular prostate cancer: correlation with histopathology.

    Science.gov (United States)

    Styles, Colin; Ferris, Nicholas; Mitchell, Catherine; Murphy, Declan; Frydenberg, Mark; Mills, John; Pedersen, John; Bergen, Noelene; Duchesne, Gillian

    2014-08-01

    Prostate cancer is common and may be treated immediately or managed conservatively by observation. We sought to determine how reliable multiparametric MRI is in the detection of intraprostatic prostate cancer and what role it has in risk stratification. The histology from 38 whole mount prostate specimens was compared with preoperative multiparametric 3T MRI studies with an endorectal receiver coil in place. T1-weighted, T2-weighted, diffusion (b values 50 400 800), perfusion (Ve , Kep , Ktrans , area under the curve) and proton spectroscopic sequences were used. For cancers greater than 0.5 cc, the detection rate for combined T2-weighted imaging and diffusion-weighted imaging (DWI) was 85%. For cancers 0.1 cc-0.5 cc, the sensitivity was 52%.Per patient, false positive rate was 50% for combined T2-weighted imaging and DWI. Perfusion imaging had a sensitivity of 70% for tumours greater than 0.5 cc but had a per patient false positive rate of 80% influenced by benign prostatic hypertrophy. In only 15 patients could a satisfactory spectroscopy study be obtained. Weak correlation was found between the Gleason score and tumour size (r = 0.51), apparent diffusion coefficient (ADC) (r = -0.30) and (choline + creatine)/citrate ratio (r = 0.41). T2-weighted imaging and DWI in combination were the best strategy for detecting prostate cancer and had a sensitivity of 85% for detecting lesions greater than 0.5 cc. At 3T, an ADC threshold of between 1100-1200.10(-6)  mm(2) /s was optimal for diagnosing prostate cancer. There are significant limitations in the use of perfusion and spectroscopy to detect prostate cancer. Magnetic resonance imaging-targeted or guided biopsy post-MRI imaging is likely to be needed in some patients to assist risk stratification. © 2014 The Royal Australian and New Zealand College of Radiologists.

  3. Interação medicamentosa associada à reinternação hospitalar: estudo retrospectivo em um hospital geral

    Directory of Open Access Journals (Sweden)

    Cristiano Soares de Moura

    2012-12-01

    Full Text Available OBJETIVO: Analisar a relação entre interação medicamentosa potencial e reinternação hospitalar. MÉTODOS: Estudo retrospectivo com 1.487 pacientes maiores de 18 anos admitidos em um hospital geral em Vitória da Conquista, BA, de janeiro a dezembro de 2007. Os dados foram extraídos da Autorização de Internação Hospitalar do Sistema de Informação Hospitalar do Sistema Único de Saúde. O relacionamento probabilístico foi empregado para combinar múltiplas autorizações de uma mesma internação em um único registro e para identificar readmissões. Informações sobre prescrições foram agregadas manualmente aos registros do Sistema de Informação Hospitalar. Regressão logística foi utilizada para quantificar a influência de interação medicamentosa potencial e reinternação. Regressão de Cox foi empregada para testar a influência dessa variável no tempo até a primeira reinternação. RESULTADOS: Foram identificadas 99 readmissões (7% dos pacientes. Interação medicamentosa potencial foi encontrada em 35% das prescrições analisadas. Pacientes com potencial de interação medicamentosa na admissão prévia foram mais propensos à reinternação. A razão de chance ajustada indicou que esses pacientes tinham chance 2,4 vezes maior de readmissão; a taxa de risco ajustada mostrou que em pacientes com interação medicamentosa esse risco foi 79% maior (p < 0,01. CONCLUSÕES: Os resultados encontrados neste trabalho sugerem associação entre exposição à interação em internação prévia e risco aumentado de reinternação. Os profissionais de saúde devem atentar para os riscos potenciais de certas combinações medicamentosas e monitorar cuidadosamente pacientes em maior risco, como aqueles com insuficiência renal ou idosos.

  4. Interações fármaco-alimento/nutriente potenciais em pacientes pediátricos hospitalizados

    Directory of Open Access Journals (Sweden)

    Everton Moraes Lopes

    2013-01-01

    Full Text Available A alimentação e a administração de medicamentos são indispensáveis na rotina hospitalar, porém sua interação pode acarretar prejuízos ao estado nutricional ou ao tratamento farmacológico, principalmente em crianças, uma vez que possuem certas particularidades. Por isso, profissionais da saúde devem aumentar a atenção durante a administração de fármacos, sobretudo os enfermeiros, por estarem envolvidos diretamente nesse processo. O objetivo desse estudo foi investigar as possíveis interações entre medicamentos e alimentos/nutrientes na unidade de pediatria em um hospital piauiense. Tratou-se de um estudo descritivo, transversal, com abordagem quantitativa, realizado com 90 pacientes hospitalizados. A amostra apresentou prevalência do sexo masculino (55,6% e uma média de idade de 62,76 meses (± 44,101. Os antimicrobianos tiveram uma frequência significativa, com 79 interações entre os medicamentos estudados, seguidos pelos corticoides com 22 ocorrências. Foram encontrados ainda, nove episódios de interações envolvendo os antiulcerosos. Entre os nutrientes, a vitamina B12 teve sua biodisponibilidade reduzida por muitos fármacos. Em suma, pode ser observado que as interações estão presentes na clínica pediátrica, e só o conhecimento pode minimizar prejuízos e/ou multiplicar os benefícios decorrentes das associações de medicamentos com alimentos. Assim, esse trabalho surge como um indicador de segurança do paciente, uma vez que identifica erros de medicação (interações entre prescrições médicas e a da equipe de nutrição.

  5. Interação Universidade-Empresa: o caso do setor sucroalcooleiro de Pernambuco

    Directory of Open Access Journals (Sweden)

    Fagner Diego Spíndola

    2015-04-01

    Full Text Available O trabalho apresenta um panorama atual da inovação no setor sucroalcooleiro pernambucano, cujo objetivo foi melhor entender as interações das empresas com as universidades e institutos públicos de pesquisa que têm promovido alguma inovação no setor. Adicionalmente, testou-se a hipótese, através do estudo de caso, de que o segmento supracitado preservou traços de sua história que legaram uma postura de setor pouco inovativo e dependente de proteção governamental. Parte de pesquisa mais ampla, o presente trabalho resulta de estudo de caso em que foram entrevistados agentes envolvidos com a interação U-E em usinas de açúcar, na instituição patronal e em instituições de pesquisa em Pernambuco. O estudo permitiu verificar que a inovação nesse setor ocorre de forma distinta em cada um dos três diferentes segmentos em que está subdividido: agricultura, indústria e transporte e mecanização. Na área agrícola, as empresas introduzem inovações que, no entanto, são desenvolvidas por instituições públicas de pesquisa existentes no estado, como a Ridesa e o Cetene, para quem as usinas e os pequenos produtores transferem esforços em P&D. Na área industrial e na de transporte e mecanização, as inovações são introduzidas por intermédio do sindicato patronal e de empresas de consultoria técnica que as importam de outras regiões ou países, enquanto o desenvolvimento de novos produtos não foi observado na indústria sucroalcooleira pernambucana. O foco das usinas locais é o mercado externo de açúcar por possuírem uma vantagem comparativa logística e contarem internamente com grande concorrência de produtores do Centro-Sul. Essa exportação do açúcar tem sido feita a granel, levando a crer que os produtores têm passado adiante parte importante da agregação de valor ao produto, deixando os procedimentos mais específicos, como criação de produtos finais diferenciados, para o importador e, por isso

  6. Trazodona: farmacologia e interações medicamentosas Trazodone: pharmacology and drug interactions

    Directory of Open Access Journals (Sweden)

    Maria da Graça Cantarelli

    2006-01-01

    Full Text Available CONTEXTO: A trazodona, substância com potencial antidepressivo e ansiolítico, continua sendo uma opção terapêutica, inclusive como indutor do sono. OBJETIVO: Recuperar informações e buscar novos conhecimentos sobre sua farmacocinética e sua farmacodinâmica e, assim, melhor compreender seu perfil de interações medicamentosas, aumentando a segurança em seu uso. MÉTODO: Esta revisão foi realizada por meio de busca eletrônica nos bancos de dados Medline e HighWire Press, utilizando como palavras-chave "trazodone", "adverse drug reaction" e "drug interaction". Eventualmente, informações complementares foram extraídas de bibliografia pesquisada e de livros-texto de farmacologia. RESULTADOS: Foram encontradas importantes informações sobre os perfis farmacocinético e farmacodinâmico da trazodona, mostrando o interesse mantido de pesquisadores. CONCLUSÕES: A trazodona tem se renovado como uma opção não apenas em depressão e insônia, mas também em certas disfunções sexuais, demonstrando um padrão de interações favorável e um perfil de reações adversas que pode ser contornado se bem avaliado e conduzido.BACKGROUND: Trazodone is an atypical antidepressant with further therapeutic effects on anxiety and sleep disorders. OBJECTIVE: To review the pharmacokinetic and pharmacodynamic properties of trazodone in order to better understand the potential of adverse events related to drug interactions. METHODS: Electronic search of Medline and High Wire Press databases using the keywords: "trazodone", "adverse drug reaction", and "drug interaction". Additional references were obtained from pharmacology textbooks. RESULTS: A comprehensive data set on the pharmacological profile of trazodone was obtained. CONCLUSION: Trazodone is being increasedly prescribed no only to depressive and sleep disorders, but also in certain sexual dysfunctions. The risk of adverse events due to drug interactions is acceptable and manageable, rendering

  7. Interação social e o comportamento da investidora brasileira

    Directory of Open Access Journals (Sweden)

    José Fajardo

    2010-09-01

    Full Text Available No presente artigo analisamos as características de mulheres que investem em ações no mercado brasileiro, seja diretamente, ou indiretamente, através de fundos de ações. Nosso objetivo é verificar se além dos fatores como idade, escolaridade, estado civil e patrimônio, há evidências de que a interação social aumenta a participação da mulher no mercado acionário. Através de um questionário enviado pela Internet para um grupo de investidoras, organizamos uma base de dados com variáveis determinantes na tomada de decisão de investimentos e analisamos as significâncias destas nas diversas combinações de regressões realizadas. Aplicamos o método Probit e calculamos os efeitos marginais sobre a variável dependente. Encontramos evidências que além do patrimônio financeiro, o nível de escolaridade o estado civil, sintomas de depressão e alguns indicadores de interação social são estatisticamente significantes quando a mulher escolhe seus investimentos. Também, como proposto por vários outros estudos, a propensão ao risco é determinante na escolha dos investimentos.In the present paper we analyze the characteristics of women that invest in the Brazilian capital market, directly or indirectly, through stock funds. Our goal is to verify if, in addition to the usual factors like age, years of education, marital status and wealth, there is evidence that social interactions increase the participation of women in capital markets. Through a questionnaire sent by e-mail to a group of women investors, we organize a data base with variables that determines the investment decision and analyze theirstatistical significance, we use a Probity model and compute the marginal effects. We find evidence that additionally to wealth, years of education and marital status, depression and other indicators of social interaction are statistically significant when woman decides their investments. Also, as suggested by several works, risk

  8. Langerhans cells are not required for epidermal V gamma 3 T cell homeostasis and function

    NARCIS (Netherlands)

    Taveirne, Sylvie; De Colvenaer, Veerle; Van Den Broeck, Tina; Van Ammel, Els; Bennett, Clare L.; Taghon, Tom; Vandekerckhove, Bart; Plum, Jean; Clausen, Bjorn E.; Kaplan, Daniel H.; Leclercq, Georges

    2011-01-01

    This study tested the hypothesis that V gamma 3 TCR-bearing T cells are influenced by LCs. V gamma 3 T cells and LCs are located in the epidermis of mice. V gamma 3 T cells represent the main T cell population in the skin epithelium and play a crucial role in maintaining the skin integrity, whereas

  9. Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

    Directory of Open Access Journals (Sweden)

    Jun Ueda

    2017-01-01

    Full Text Available Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.

  10. Prospective comparison of late 3T MRI with conventional angiography in evaluating the patency of cerebral arteriovenous malformations treated with stereotactic radiosurgery

    Energy Technology Data Exchange (ETDEWEB)

    Khandanpour, Nader [National Hospital for Neurology and Neurosurgery, London (United Kingdom); Griffiths, Paul; Hoggard, Nigel [University of Sheffield, Academic Unit of Radiology, C Floor, Royal Hallamshire Hospital, Sheffield (United Kingdom); Warren, Daniel [University of Sheffield, C Floor, Royal Hallamshire Hospital, Sheffield (United Kingdom)

    2013-06-15

    Risk of further haemorrhage in patients suffering from arteriovenous malformation (AVM) would be eliminated only if complete obliteration of the AVM is obtained. Therefore, these patients frequently need long-term follow-up. Conventional catheter angiography (CCA) with a risk of 0.5 %.to 1.6 % of significant neurological complications has traditionally been used for this purpose. However, magnetic resonance imaging (MRI) at 3T may be a safer alternative. The aim of this study was to evaluate if MRI at 3T can accurately evaluate closure of AVM in 2 years after stereotactic radiosurgery. Twenty-three patients with both MRI at 3T and a CCA study were examined. The residual AVMs were evaluated by MRI at 3T against CCA in a prospective study. The time interval between radiosurgery and neuroimaging was on average of 25 months (range, 15-30 months) for MRI study and 33 months (range, 25-46 months) for CCA study. Ten patients showed closure of the AVM on MRI, all of which were confirmed on CCA. There was a complete agreement between late MRI at 3T scan and CCA in evaluation of AVM patency. (orig.)

  11. Interações modais não ressonantes em vigas cantilever flexíveis

    OpenAIRE

    Barros, Everaldo de [UNESP

    2004-01-01

    Na presença de não linearidades, a resposta forçada de estruturas exibe diversos fenômenos físicos que não podem ser descritos através de modelos lineares. Estes fenômenos incluem ressonâncias sub-harmônicas, ressonâncias super-harmônicas, jumps , movimentos quasi-periódicos, movimentos de período múltiplo, caos e interações modais. Recentes estudos experimentais indicam que um novo tipo de interação modal pode ocorrer através de mecanismos não ressonantes, decorrente da transferência de ene...

  12. InteraÃÃo medicamentosa: conhecimento dos enfermeiros de um hospital pÃblico do PiauÃ.

    OpenAIRE

    Laura Maria Feitosa Formiga

    2012-01-01

    A assistÃncia de enfermagem prestada com qualidade està baseada tanto no cuidado prestado, como na garantia de uma terapÃutica medicamentosa segura. Portanto o conhecimento das propriedades bÃsicas e das aÃÃes farmacolÃgicas dos medicamentos à fundamental na prÃtica de enfermagem na avaliaÃÃo prÃ-administrativa, na dose e administraÃÃo, na avaliaÃÃo e promoÃÃo dos efeitos terapÃuticos, na identificaÃÃo e reduÃÃo dos efeitos adversos e interaÃÃes medicamentosas. Dessa forma as interaÃÃes medic...

  13. MAGNET

    CERN Multimedia

    Benoit Curé

    The magnet subsystems resumed operation early this spring. The vacuum pumping was restarted mid March, and the cryogenic power plant was restarted on March 30th. Three and a half weeks later, the magnet was at 4.5 K. The vacuum pumping system is performing well. One of the newly installed vacuum gauges had to be replaced at the end of the cool-down phase, as the values indicated were not coherent with the other pressure measurements. The correction had to be implemented quickly to be sure no helium leak could be at the origin of this anomaly. The pressure measurements have been stable and coherent since the change. The cryogenics worked well, and the cool-down went quite smoothly, without any particular difficulty. The automated start of the turbines had to be fine-tuned to get a smooth transition, as it was observed that the cooling power delivered by the turbines was slightly higher than needed, causing the cold box to stop automatically. This had no consequence as the cold box safety system acts to keep ...

  14. MAGNET

    CERN Multimedia

    B. Curé

    During the winter shutdown, the magnet subsystems went through a full maintenance. The magnet was successfully warmed up to room temperature beginning of December 2008. The vacuum was broken later on by injecting nitrogen at a pressure just above one atmosphere inside the vacuum tank. This was necessary both to prevent any accidental humidity ingress, and to allow for a modification of the vacuum gauges on the vacuum tank and maintenance of the diffusion pumps. The vacuum gauges had to be changed, because of erratic variations on the measurements, causing spurious alarms. The new type of vacuum gauges has been used in similar conditions on the other LHC experiments and without problems. They are shielded against the stray field. The lubricants of the primary and diffusion pumps have been changed. Several minor modifications were also carried out on the equipment in the service cavern, with the aim to ease the maintenance and to allow possible intervention during operation. Spare sensors have been bought. Th...

  15. Formas de interação entre humanos e dados digitais em ambientes virtuais

    Directory of Open Access Journals (Sweden)

    Luiz Antônio Rocha

    2004-06-01

    Full Text Available Este texto visa debruçar-se sobre a relação homem/meio digital e sobre as possibilidades dessa interação. Partimos da seguinte questão, quais as chances dessa relação vir a ser um meio que apresente ferramentas de operação e criação, possibilitando a geração de novas informações, a partir daquelas já disponibilizadas em ambiente virtual, e um ciclo contínuo? Que possibilidades que existiriam de se criar informação a partir da operação com a visualização de dados, invertendo a relação de dependência que a representação tem sobre os dados que traduz. No que isto poderia vir a ampliar o fazer artístico.This article points to the relationship between humans and information (database in virtual collaborative environments and it questions about the possibilities that could come out from this interaction. If this can lead to a new process of creation based on the direct operation through the displayed information, feeding back the database, and, the aftermath that could reflect on the artistic work in the digital realm. What are the possibilities of creating, backwarding the process, from the manipulation of information back to the database.

  16. Interação de fatores riscos em tuberculose Interaction of factors on pulmonary tuberculosis

    Directory of Open Access Journals (Sweden)

    Antônio Ruffino-Netto

    1979-06-01

    Full Text Available Estudou-se o possível sinergismo dos fatores alcoolismo e tabagismo na tuberculose pulmonar, a partir de dados de um estudo caso-controle onde 854 pessoas foram entrevistadas (sendo 427 casos-pacientes portadores de tuberculose pulmonar e 427 controles e questionadas quanto ao hábito de ingestão alcoólica e o de fumar em períodos precedendo ao diagnóstico da doença. Concluiu-se que existe pequena interação destes fatores na tuberculose pulmonar; o hábito de fumar estaria relacionado com a doença tuberculose através de sua associação com a ingestão de bebidas alcoólicas.A case-control study was made of the possible synergetic effect of smoking and alcohol drinking on pulmonary tuberculosis. Analysis of the results of 854 interviews showed that there is little interactive effect in the multiplicative sense (synergism between these factors and pulmonary tuberculosis. It is suggested that the habit of smoking may be related to pulmonary tuberculosis only through its association with that of alcohol drinking.

  17. Referenciação e interação em blogs

    Directory of Open Access Journals (Sweden)

    Vanda Maria da Silva Elia

    2010-07-01

    Full Text Available http://dx.doi.org/10.5007/1807-9288.2008v4n2p63Neste artigo, analisamos os diários on line ou blogs com o objetivo de verificar como se constitui nesse espaço de escrita digital o processo de referenciação constituído por meio de expressões nominais. Para a análise pretendida, selecionamos, aleatoriamente, blogs produzidos no contexto empresarial e verificamos, nos comentários dos leitores, as expressões nominais utilizadas com a função de retomar referentes construídos na mensagem postada pelo escritor/blogueiro. Os resultados revelaram que os leitores/comentaristas retomam referentes introduzidos na mensagem postada pelo blogueiro e os mantêm ativados no texto, garantindo não só a manutenção temática, como também a progressão do texto/discurso, por meio das formas nominais referenciais que utilizam. A pesquisa reforçao postulado segundo o qual a referenciação é uma atividade discursiva com relevante papel na organização do texto, de modo a contribuir, efetivamente, para a interação e produçãode sentido.

  18. Um estudo ecológico sobre as interações da família com o abrigo

    Directory of Open Access Journals (Sweden)

    Queila Almeida Vasconcelos

    2009-08-01

    Full Text Available O presente estudo teve por objetivo investigar as interações entre famílias e as instituições de abrigo a partir do modelo bioecológico de desenvolvimento humano de Urie Bronfenbrenner. Para tanto, foram acompanhadas as interações de funcionários de uma instituição do extremo sul do Brasil e a família de uma criança institucionalizada durante o processo de reinserção familiar. Os profissionais revelaram crenças e percepções idealizadas que prescrevem as expectativas dos modos de vida da família nuclear tradicional. Por sua vez, a família apresentou temores em relação aos julgamentos dos representantes da instituição pela possibilidade de perder a guarda dos outros filhos não-institucionalizados. Portanto, os discursos da família e da instituição revelaram interações que evidenciam desconfiança entre os dois contextos, o que dificulta e prorroga o retorno das crianças ao ambiente familiar.

  19. Interação Estratégica e Concursos Públicos: Uma Etnografia do Concurso da Magistratura Francesa

    Directory of Open Access Journals (Sweden)

    Fernando de Castro Fontainha

    2015-12-01

    Full Text Available Este artigo propõe um quadro analítico para os concursos públicos a partir da sociologia interacionista, sobretudo dos trabalhos do sociólogo Erving Goffman. Num primeiro momento, mostra-se como o construtivismo avançou em direção à compreensão dos processos de competição de atores, notadamente as competições escolares. Em seguida, analisa-se a recepção da Teoria dos Jogos pela Sociologia para avançar, posteriormente, no olhar goffmaniano sobre as dinâmicas interacionais: começando pelas interações cotidianas (metáfora do teatro, passando pelas interações focalizadas e desembocando nas interações estratégicas (metáfora do jogo. Por fim, uma etnografia da prova oral de cultura geral da magistratura francesa permite que as categorias teóricas trabalhadas sejam aplicadas através da descrição densa de quadros interacionais.

  20. Surto psicótico pela possível interação medicamentosa de sibutramina com finasterida

    Directory of Open Access Journals (Sweden)

    Sucar Douglas Dogol

    2002-01-01

    Full Text Available É descrito um caso de interação medicamentosa em um paciente de 30 anos, que fazia com sucesso um tratamento antiobesidade com sibutramina. Esse medicamento é um saciogênico que inibe a recaptação da serotonina e da noradrenalina. Logo após a introdução da finasterida para tratamento da alopécia androgênica, o paciente passou a apresentar surto psicótico. Esse medicamento é um potente inibidor da 5-alfa-redutase do tipo II. A interação medicamentosa demonstra-se por meio de um rigoroso acompanhamento clínico e de uma aplicação do algoritmo de Naranjo. Também descrevem-se as condições clínicas gerais do paciente e sua evolução. Discutem-se os mecanismos prováveis que levaram à interação medicamentosa, com a conclusão de que a finasterida inibiu o metabolismo hepático da sibutramina. Esta deslocou a finasterida de seus pontos de ligações com as proteínas, aumentando sua fração livre plasmática e inibindo ainda mais o metabolismo da sibutramina. Com aumento de serotonina, noradrenalina e dopamina na fenda sináptica, desencadeia-se o surto psicótico.

  1. Interação educadora-criança em creches públicas: estilos linguísticos

    Directory of Open Access Journals (Sweden)

    Deborah Dornellas Ramos

    2012-03-01

    Full Text Available O presente estudo teve o objetivo de analisar a interação educadora-criança em creches públicas, considerando os estilos linguísticos das educadoras e a comunicação infantil. O estudo dos estilos linguísticos destaca-se nas análises das interações entre adultos e crianças, especialmente em se tratando daquelas da faixa etária em torno dos 24 meses, devido à emergência das primeiras pequenas frases. Participaram do estudo seis educadoras e doze crianças com idade entre 23 e 25 meses, que frequentavam creches públicas de João Pessoa, Paraíba. As interações entre educadora e criança foram observadas sistematicamente em contextos diádicos e poliádicos de leitura. Categorias interacionais foram elaboradas a posteriori, para a análise dos dados. As educadoras utilizaram mais estilos associados à promoção do desenvolvimento linguístico nos contextos diádicos, enquanto os estilos relacionados à direção e ao controle dos comportamentos predominaram nos contextos poliádicos. As análises consideraram a influência mútua entre os estilos comunicativos e o nível de desenvolvimento linguístico infantil, além dos aspectos contextuais.

  2. Interação fármaco-nutriente em unidade de terapia intensiva: revisão da literatura e recomendações atuais

    Directory of Open Access Journals (Sweden)

    Tatiane Heldt

    2013-06-01

    Full Text Available OBJETIVO: Descrever as interações entre fármacos e nutriente e sua frequência nas unidades de terapia intensiva bem como avaliar o grau de consciência a esse respeito por parte da equipe de profissionais. MÉTODOS: Foram revisados, na base de dados eletrônica PubMed, especificamente no MeSH, os unitermos: "drug interactions" e "nutrition therapy". Os estudos foram sistematicamente revisados para a descrição de tipos de interações entre fármacos e nutrientes, suas frequências e consequências. RESULTADOS: Foram encontrados 67 artigos. Dentre estes, 20 artigos estavam adequados à metodologia adotada e atingiram os objetivos do estudo. Destes, 14 artigos descreviam interações entre fármacos e nutrição enteral, 3 descreviam interações entre fármacos e nutrição parenteral, e 3 descreviam a importância e os cuidados para evitar tais interações. CONCLUSÃO: A literatura referente a interações entre fármacos e nutrientes é escassa e sugere a fragilidade das equipes assistenciais em reconhecer o potencial para interações. Possivelmente a construção de um protocolo para avaliação de interação fármaco-nutriente aumente a segurança e eficácia dos processos terapêuticos.

  3. Three dimensional first-pass myocardial perfusion imaging at 3T: feasibility study

    Directory of Open Access Journals (Sweden)

    Pohost Gerald M

    2008-12-01

    Full Text Available Abstract Background In patients with ischemic heart disease, accurate assessment of the extent of myocardial perfusion deficit may be important in predicting prognosis of clinical cardiac outcomes. The aim of this study was to compare the ability of three dimensional (3D and of two dimensional (2D multi-slice myocardial perfusion imaging (MPI using cardiovascular magnetic resonance (CMR in determining the size of defects, and to demonstrate the feasibility of 3D MPI in healthy volunteers at 3 Tesla. Methods A heart phantom was used to compare the accuracy of 3D and 2D multi-slice MPI in estimating the volume fraction of seven rubber insets which simulated transmural myocardial perfusion defects. Three sets of cross-sectional planes were acquired for 2D multi-slice imaging, where each set was shifted along the partition encoding direction by ± 10 mm. 3D first-pass contrast-enhanced (0.1 mmol/kg Gd-DTPA MPI was performed in three volunteers with sensitivity encoding for six-fold acceleration. The upslope of the myocardial time-intensity-curve and peak SNR/CNR values were calculated. Results Mean/standard deviation of errors in estimating the volume fraction across the seven defects were -0.44/1.49%, 2.23/2.97%, and 2.59/3.18% in 3D, 2D 4-slice, and 2D 3-slice imaging, respectively. 3D MPI performed in healthy volunteers produced excellent quality images with whole left ventricular (LV coverage. Peak SNR/CNR was 57.6 ± 22.0/37.5 ± 19.7 over all segments in the first eight slices. Conclusion 3D performed better than 2D multi-slice MPI in estimating the size of perfusion defects in phantoms. Highly accelerated 3D MPI at 3T was feasible in volunteers, allowing whole LV coverage with excellent image quality and high SNR/CNR.

  4. Commissioning and quality control of a dedicated wide bore 3T MRI simulator for radiotherapy planning

    Directory of Open Access Journals (Sweden)

    Aitang Xing

    2016-06-01

    Full Text Available Purpose: The purpose of this paper is to describe a practical approach to commissioning and quality assurance (QA of a dedicated wide-bore 3 Tesla (3T magnetic resonance imaging (MRI scanner for radiotherapy planning.Methods: A comprehensive commissioning protocol focusing on radiotherapy (RT specific requirements was developed and performed. RT specific tests included: uniformity characteristics of radio-frequency (RF coil, couch top attenuation, geometric distortion, laser and couch movement and an end-to-end radiotherapy treatment planning test. General tests for overall system performance and safety measurements were also performed.Results: The use of pre-scan based intensity correction increased the uniformity from 61.7% to 97% (body flexible coil, from 50% to 90% (large flexible coil and from 51% to 98% (small flexible coil. RT flat top couch decreased signal-to-noise ratio (SNR by an average of 42%. The mean and maximum geometric distortion was found to be 1.25 mm and 4.08 mm for three dimensional (3D corrected image acquisition, 2.07 mm and 7.88 mm for two dimensional (2D corrected image acquisition over 500 mm × 375 mm × 252 mm field of view (FOV. The accuracy of the laser and couch movement was less than ±1 mm. The standard deviation of registration parameters for the end-to-end test was less than 0.41 mm. An on-going QA program was developed to monitor the system’s performance.Conclusion: A number of RT specific tests have been described for commissioning and subsequent performance monitoring of a dedicated MRI simulator (MRI-Sim. These tests have been important in establishing and maintaining its operation for RT planning.

  5. Assessment of subchondral bone marrow lipids in healthy controls and mild osteoarthritis patients at 3T.

    Science.gov (United States)

    Wang, Ligong; Salibi, Nouha; Chang, Gregory; Vieira, Renata L R; Babb, James S; Krasnokutsky, Svetlana; Abramson, Steven; Regatte, Ravinder R

    2012-04-01

    The compartment-specific lipid changes in femoral-tibial bone of healthy controls and mild osteoarthritis (OA) patients were quantified at 3.0 T. Healthy volunteers [Kellgren-Lawrence (KL) grade = 0; n = 15, 4 females, 11 males, mean age 39 ± 16 years, age range = 24-78 years] and mild OA patients (KL = 1, 2; n = 26, 12 females, 14 males, mean age 61 ± 14 years, age range = 27-80 years) were scanned on a 3 T scanner. Clinical proton density (PD)-weighted fast spin echo (FSE) images in the sagittal (without fat-saturation), axial and coronal (fat-saturation) planes were acquired for cartilage Whole-Organ MR Imaging Score (WORMS) grading. A voxel of 10 × 10 × 10 mm(3) was positioned in the medial and lateral compartments of the tibia [medial tibial (MT) and lateral tibial (LT)] and femur [medial femoral (MF) and lateral femoral (LF)] for MRS measurements using the single voxel-stimulated echo acquisition mode (STEAM) pulse sequence. All MRS data were processed with Java-based Magnetic Resonance User Interface (JMRUI). Wilcoxon's rank sum test and mixed model two-way analysis of variance (ANOVA) were performed to determine significant differences between different compartments as well as examine the effect of OA grade and compartment, and their interactions. Generally, the MF compartment index of unsaturation was increased in healthy subjects compared with OA subjects (whether graded by KL or WORMS score). Differences between MF at KL0 and all other compartments at KL1 except LF approached statistical significance (p saturated lipids signals could be observed predominantly in the 2.03 p.p.m. frequency shift. Healthy controls in the MF compartment had the lowest saturated lipid signals, and mild OA patients with KL2 and WORMS5-6 in the MF compartment had the highest saturated lipid signals compared with other compartments at 2.03 p.p.m. (p < 0.05).

  6. In-bore setup and Software for 3T MRI-guided Transperineal Prostate Biopsy

    Science.gov (United States)

    Tokuda, Junichi; Tuncali, Kemal; Iordachita, Iulian; Song, Sang-Eun; Fedorov, Andriy; Oguro, Sota; Lasso, Andras; Fennessy, Fiona M; Tempany, Clare M; Hata, Nobuhiko

    2012-01-01

    MRI-guided prostate biopsy in conventional closed-bore scanners requires transferring the patient outside the bore during needle insertion due to the constrained in-bore space, causing a safety hazard and limiting image feedback. To address this issue, we present our custom-made in-bore setup and software to support MRI-guided transperineal prostate biopsy in a wide-bore 3 Tesla (T) MRI scanner. The setup consists of a specially designed tabletop and a needle-guiding template with Z-frame that give a physician access to the perineum of the patient at the imaging position and allow performance of MRI-guided transperineal biopsy without moving the patient out of the scanner. The software and Z-frame allow registration of the template, target planning, and biopsy guidance. Initially, we performed phantom experiments to assess the accuracy of template registration and needle placement in a controlled environment. Subsequently, we embarked on our clinical trial (N = 10). The phantom experiments showed that the translational errors of the template registration along the right-left (RP) and anterior-posterior (AP) axes were 1.1 ± 0.8 mm and 1.4 ± 1.1 mm respectively, while the rotational errors around the RL, AP, and superior-inferior axes were 0.8 ± 1.0 degrees, 1.7 ± 1.6 degrees, and 0.0 ± 0.0 degrees respectively. The 2D root-mean-square (RMS) needle placement error was 3.0 mm. The clinical biopsy procedures were safely carried out in all ten clinical cases with a needle placement error of 5.4 mm (2D RMS). In conclusion, transperineal prostate biopsy in a wide-bore 3T scanner is feasible using our custom-made tabletop set up and software, which supports manual needle placement without moving the patient out of the magnet. PMID:22951350

  7. Increased Oxidative Stress in Cultured 3T3-L1 Cells was Attenuated by Berberine Treatment.

    Science.gov (United States)

    Dong, Shi-Fen; Yasui, Naomi; Negishb, Hiroko; Kishimoto, Aya; Sun, Jian-Ning; Ikeda, Katsumi

    2015-06-01

    The 3T3-L1 cell line is one of the most well-characterized and reliable models for studying adipocytes. Increased oxidative stress in accumulated fat was found in 3T3-L1 cells. Berberine, an isoquinoline alkaloid, could suppress fat deposition in 3T3-L1 cells; however, whether berberine suppresses increased oxidative stress is not well known. In this study, we observed the effect of berberine on increased oxidative stress in 3T3-L1 cells. 3T3-L1 cells were cultured and treated with berberine (5-20 μM) from day 3 to day 8. We confirmed that berberine markedly inhibited fat accumulation and lipid droplets in 3T3-L1 adipocytes and decreased triglyceride content. Berberine inhibited increased oxidative stress in 3T3-L1 cells by suppressing reactive oxygen species (ROS) production, and increased glutathione peroxidase (GPx) gene expression and GPx activity. Berberine also markedly reduced adipokines secreted by adipocytes, including leptin and resistin.

  8. Heterologous Expression of Rat Testis GABAA Receptor β3t Splicing Variant in CHO Cells

    Institute of Scientific and Technical Information of China (English)

    Shi-feng LI; Yu-guang CHEN; Yuan-chang YAN; Yi-ping LI

    2004-01-01

    Objective To characterize a possible retention function of unique sequence in the 5'end of rat testis GABAA receptor β3t splicing variantMethods Rat testis GABAA receptor β3t splicing variant cDNA was cloned and two eukaryotic expression recombinant plasmids of pEGFP-N1 and pEGFP-C1 were constructed respectively by fusing green fluorescent protein to the N or C-terminus of β3t isoform. The recombinant plasmids were transfected into CHO cells by calcium phosphate co-precipitation method. Fluorescence microscope and laser confocal microscope were used to analyze localization of β3t in the transfected cells. ConA-Texas-Red was used to label cell ER and the localization of rat testis β3t splicing variant in CHO cells was determined.Results When rat testis β3t splicing variant was expressed in CHO cells, two expression patterns were delineated, the distributions of uniform and mainly discrete intracellular compartments respectively. The chimera product failed to be translocated into the cell surface when expressed in CHO cells; whereas the β3 subunit of rat brain was incorporated into the plasma membrane.Conclusion The inability of β3t to target into the ER may be a consequence of the unique 25 specific amino acid segments in the N terminus.

  9. Active form Notch4 promotes the proliferation and differentiation of 3T3-L1 preadipocytes

    Energy Technology Data Exchange (ETDEWEB)

    Lai, Peng-Yeh [Institute of Molecular Biology and Department of Life Science, National Chung Cheng University, Chiayi 621, Taiwan, ROC (China); Tsai, Chong-Bin [Institute of Molecular Biology and Department of Life Science, National Chung Cheng University, Chiayi 621, Taiwan, ROC (China); Department of Ophthalmology, Chiayi Christian Hospital, Chiayi 600, Taiwan, ROC (China); Tseng, Min-Jen, E-mail: biomjt@ccu.edu.tw [Institute of Molecular Biology and Department of Life Science, National Chung Cheng University, Chiayi 621, Taiwan, ROC (China)

    2013-01-18

    Highlights: ► Notch4IC modulates the ERK pathway and cell cycle to promote 3T3-L1 proliferation. ► Notch4IC facilitates 3T3-L1 differentiation by up-regulating proadipogenic genes. ► Notch4IC promotes proliferation during the early stage of 3T3-L1 adipogenesis. ► Notch4IC enhances differentiation during subsequent stages of 3T3-L1 adipogenesis. -- Abstract: Adipose tissue is composed of adipocytes, which differentiate from precursor cells in a process called adipogenesis. Many signal molecules are involved in the transcriptional control of adipogenesis, including the Notch pathway. Previous adipogenic studies of Notch have focused on Notch1 and HES1; however, the role of other Notch receptors in adipogenesis remains unclear. Q-RT-PCR analyses showed that the augmentation of Notch4 expression during the differentiation of 3T3-L1 preadipocytes was comparable to that of Notch1. To elucidate the role of Notch4 in adipogenesis, the human active form Notch4 (N4IC) was transiently transfected into 3T3-L1 cells. The expression of HES1, Hey1, C/EBPδ and PPARγ was up-regulated, and the expression of Pref-1, an adipogenic inhibitor, was down-regulated. To further characterize the effect of N4IC in adipogenesis, stable cells expressing human N4IC were established. The expression of N4IC promoted proliferation and enhanced differentiation of 3T3-L1 cells compared with those of control cells. These data suggest that N4IC promoted proliferation through modulating the ERK pathway and the cell cycle during the early stage of 3T3-L1 adipogenesis and facilitated differentiation through up-regulating adipogenic genes such as C/EBPα, PPARγ, aP2, LPL and HSL during the middle and late stages of 3T3-L1 adipogenesis.

  10. Diagnostic Performance of Self-navigated Whole-Heart Contrast-enhanced Coronary 3-T MR Angiography.

    Science.gov (United States)

    He, Yi; Pang, Jianing; Dai, Qinyi; Fan, Zhanming; An, Jing; Li, Debiao

    2016-11-01

    Purpose To evaluate the diagnostic performance of self-navigated whole-heart coronary 3-T magnetic resonance (MR) angiography by using conventional invasive coronary angiography (ICA) as the reference gold standard. Materials and Methods This study was approved by the local ethics committee. Written informed consent was obtained from each patient before the study. Thirty-nine consecutive patients underwent coronary MR angiography and later underwent ICA. Coronary MR angiography was performed with a 3-T imager with contrast agent enhancement during free breathing with self-navigated affine motion correction reconstruction. Coronary segments with reference diameters larger than 1.5 mm were included in the comparison between coronary MR angiography and ICA. The coronary MR angiography images were evaluated by two experienced readers blinded to the ICA results to identify significant luminal narrowing (>50% diameter reduction in reference ICA). Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were performed to detect significant coronary artery stenosis. Results Coronary MR angiography examinations were successfully performed in all 39 patients. A total of 327 coronary segments had reference luminal diameter larger than 1.5 mm. Of these 327 coronary segments, 303 (92.7%) segments had a quality score greater than 1 at coronary MR angiography and were included in the analysis. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 78.2%, 75.0%, 81.8%, 70.6%, and 76.9%, respectively, on a per-patient basis. Conclusion Contrast-enhanced self-navigated coronary 3-T MR angiography is a promising technique for the noninvasive detection of clinically significant coronary stenosis. (©) RSNA, 2016.

  11. MAGNET

    CERN Multimedia

    Benoit Curé

    2013-01-01

    Maintenance work and consolidation activities on the magnet cryogenics and its power distribution are progressing according to the schedules. The manufacturing of the two new helium compressor frame units has started. The frame units support the valves, all the sensors and the compressors with their motors. This activity is subcontracted. The final installation and the commissioning at CERN are scheduled for March–April 2014. The overhauls of existing cryogenics equipment (compressors, motors) are in progress. The reassembly of the components shall start in early 2014. The helium drier, to be installed on the high-pressure helium piping, has been ordered and will be delivered in the first trimester of 2014. The power distribution for the helium compressors in SH5 on the 3.3kV network is progressing. The 3.3kV switches, between each compressor and its hot spare compressor, are being installed, together with the power cables for the new compressors. The 3.3kV electrical switchboards in SE5 will ...

  12. MAGNET

    CERN Multimedia

    Benoit Curé

    The cooling down to the nominal temperature of 4.5 K was achieved at the beginning of August, in conjunction with the completion of the installation work of the connection between the power lines and the coil current leads. The temperature gradient on the first exchanger of the cold box is now kept within the nominal range. A leak of lubricant on a gasket of the helium compressor station installed at the surface was observed and several corrective actions were necessary to bring the situation back to normal. The compressor had to be refilled with lubricant and a regeneration of the filters and adsorbers was necessary. The coil cool down was resumed successfully, and the cryogenics is running since then with all parameters being nominal. Preliminary tests of the 20kA coil power supply were done earlier at full current through the discharge lines into the dump resistors, and with the powering busbars from USC5 to UXC5 without the magnet connected. On Monday evening August 25th, at 8pm, the final commissionin...

  13. Magnetic

    Science.gov (United States)

    Aboud, Essam; El-Masry, Nabil; Qaddah, Atef; Alqahtani, Faisal; Moufti, Mohammed R. H.

    2015-06-01

    The Rahat volcanic field represents one of the widely distributed Cenozoic volcanic fields across the western regions of the Arabian Peninsula. Its human significance stems from the fact that its northern fringes, where the historical eruption of 1256 A.D. took place, are very close to the holy city of Al-Madinah Al-Monawarah. In the present work, we analyzed aeromagnetic data from the northern part of Rahat volcanic field as well as carried out a ground gravity survey. A joint interpretation and inversion of gravity and magnetic data were used to estimate the thickness of the lava flows, delineate the subsurface structures of the study area, and estimate the depth to basement using various geophysical methods, such as Tilt Derivative, Euler Deconvolution and 2D modeling inversion. Results indicated that the thickness of the lava flows in the study area ranges between 100 m (above Sea Level) at the eastern and western boundaries of Rahat Volcanic field and getting deeper at the middle as 300-500 m. It also showed that, major structural trend is in the NW direction (Red Sea trend) with some minor trends in EW direction.

  14. In vivo micro-CT imaging of rat brain glioma: a comparison with 3T MRI and histology.

    Science.gov (United States)

    Engelhorn, Tobias; Eyupoglu, Ilker Y; Schwarz, Marc A; Karolczak, Marek; Bruenner, Holger; Struffert, Tobias; Kalender, Willi; Doerfler, Arnd

    2009-07-10

    The aim of this study was to evaluate the potential of a novel micro-CT system to image in vivo the extent of tumor in a rat model of malignant glioma compared to 3T magnetic resonance imaging (MRI) and histology. Fourteen animals underwent double dose contrast-enhanced imaging with micro-CT and 3T MRI using a clinical machine at day 10 after stereotactic F98 glioma cell implantation. Calculation of the volume of the contrast-uptaking part of the tumor was done by manually outlining the tumor contours by two experienced neuroradiologists. The micro-CT- and MRI-derived tumor volumes were compared to histology as gold standard (hematoxylin and eosin staining and fluorescence staining). There was high interobserver reability regarding the tumor volumes (Crombach's alpha>0.81). Also, there was good correlation of micro-CT- and high-field MRI-derived tumor volumes compared to histology: 72+/-21 mm3 and 69+/-23 mm3 compared to 81+/-14 mm3, respectively (r>0.76). Both the micro-CT- and MRI-derived tumor volumes were not significantly smaller compared to histology (P>0.14). In conclusion, micro-CT allows in vivo imaging of the contrast-enhancing part of experimental gliomas with an accuracy comparable to high-field MRI.

  15. Complete Genome Sequence of the Novel Leech Symbiont Mucinivorans hirudinis M3T

    OpenAIRE

    Nelson, Michael C.; Bomar, Lindsey; Graf, Joerg

    2015-01-01

    Mucinivorans hirudinis M3T was isolated from the digestive tract of the medicinal leech, Hirudo verbana, and is the type species of a new genus within the Rikenellaceae. Here, we report the complete annotated genome sequence of this bacterium.

  16. Complete Genome Sequence of the Novel Leech Symbiont Mucinivorans hirudinis M3T.

    Science.gov (United States)

    Nelson, Michael C; Bomar, Lindsey; Graf, Joerg

    2015-02-05

    Mucinivorans hirudinis M3(T) was isolated from the digestive tract of the medicinal leech, Hirudo verbana, and is the type species of a new genus within the Rikenellaceae. Here, we report the complete annotated genome sequence of this bacterium.

  17. The effects of Ganoderma lucidum herba pharmacopuncture on 3T3-L1 preadipocyte differentiation

    Directory of Open Access Journals (Sweden)

    Chea-woo Lee

    2008-09-01

    Full Text Available Objective : The purpose of this study is to investigate the effects of Ganoderma lucidum herba pharmacopuncture (GHP on the adipogenesis in 3T3-L1 preadipocytes. Methods : 3T3- L1 preadipocytes were differentiated with adipogenic reagents by incubating for 2 days in the absence or presence of GHP ranging from 1 and 2%. The effect of GHP on cell proliferation of 3T3-L1 preadipocytes was investigated using MTT assay. The effect of GHP on adipogenesis was examined by Oil red O staining and measuring glycerol-3-phosphate dehydrogenase (GPDH and intracellular triglyceride (TG content. Results : Following results were obtained from the preadipocyte proliferation and adipocyte differentiation of 3T3-L1. We observed no effect of GHP on preadipocyte proliferation. GHP inhibited adipogenesis, the activity of GPDH and accumulation of intracellular TG content. Conclusions : These results suggest that GHP inhibit differentiation of preadipocyte.

  18. Computational dosimetry of induced electric fields during realistic movements in the vicinity of a 3 T MRI scanner.

    Science.gov (United States)

    Laakso, Ilkka; Kännälä, Sami; Jokela, Kari

    2013-04-21

    Medical staff working near magnetic resonance imaging (MRI) scanners are exposed both to the static magnetic field itself and also to electric currents that are induced in the body when the body moves in the magnetic field. However, there are currently limited data available on the induced electric field for realistic movements. This study computationally investigates the movement induced electric fields for realistic movements in the magnetic field of a 3 T MRI scanner. The path of movement near the MRI scanner is based on magnetic field measurements using a coil sensor attached to a human volunteer. Utilizing realistic models for both the motion of the head and the magnetic field of the MRI scanner, the induced fields are computationally determined using the finite-element method for five high-resolution numerical anatomical models. The results show that the time-derivative of the magnetic flux density (dB/dt) is approximately linearly proportional to the induced electric field in the head, independent of the position of the head with respect to the magnet. This supports the use of dB/dt measurements for occupational exposure assessment. For the path of movement considered herein, the spatial maximum of the induced electric field is close to the basic restriction for the peripheral nervous system and exceeds the basic restriction for the central nervous system in the international guidelines. The 99th percentile electric field is a considerably less restrictive metric for the exposure than the spatial maximum electric field; the former is typically 60-70% lower than the latter. However, the 99th percentile electric field may exceed the basic restriction for dB/dt values that can be encountered during tasks commonly performed by MRI workers. It is also shown that the movement-induced eddy currents may reach magnitudes that could electrically stimulate the vestibular system, which could play a significant role in the generation of vertigo-like sensations reported

  19. O empreendedorismo acadêmico no contexto da interação Universidade - Empresa - Governo

    Directory of Open Access Journals (Sweden)

    Ana Sílvia Rocha Ipiranga

    Full Text Available No contexto da cooperação entre universidade, empresa e governo sobressaem-se os conceitos complementares de "universidade empreendedora" e "empreendedorismo acadêmico", nos quais uma instituição acadêmica vai além da formação, desempenhando um papel no Sistema de Inovação e no desenvolvimento do país. Considerando essa discussão, este estudo tem como objetivo abordar a questão da capacitação da universidade para promover a cooperação, tendo como base as seguintes questões de pesquisa: Como ocorre a cooperação da universidade com as empresas e o governo e desses com a universidade? Quais as barreiras e vantagens para os entes participantes? Que contratos e arranjos são articulados para a concretização dessa interação? A metodologia de natureza qualitativa faz uso do caso Parque de Desenvolvimento Tecnológico (PADETEC ligado a uma universidade federal, enquanto instituição situada na fronteira dessas relações. A análise dos dados baseou-se na técnica da análise temática que se insere no conjunto das técnicas da Análise de Conteúdo. A análise evidenciou que há atuação de uma universidade empreendedora, mas, sobretudo, o empreendedorismo acadêmico dá sinais de existência; porém, é preciso ainda percorrer um longo caminho para se consolidar estes conceitos e, em consequência, a complementaridade entre suas respectivas práticas.

  20. A escolha amorosa e interação conjugal na heterossexualidade e na homossexualidade

    Directory of Open Access Journals (Sweden)

    Féres-Carneiro Terezinha

    1997-01-01

    Full Text Available Este estudo tem como objetivo investigar o processo de escolha amorosa em homens e mulheres hetero e homossexuais das camadas médias da população, de diferentes faixas etárias, em diversas situações amorosas - namoro, casamento (coabitação estável, separação e recasamento (segunda ou subsequente coabitação estável; as características das relações dos conflitos conjugais por eles estabelecidas; e o papel atribuído à terapia de casal na resolução dos conflitos conjugais. Foram estudados dois grupos: 240 heterossexuais (53 solteiros, 64 casados, 63 separados e 60 recasados e 116 homossexuais (49 solteiros, 36 casados, 19 separados e 12 recasados. Na avaliação da escolha amorosa foram utilizados 26 atributos dispostos em escalas de avaliação de 5 pontos, tal como proposto por Buss (1989, e entrevistas semi-estruturadas a partir de Féres-Carneiro (1987, contendo tópicos referentes a dimensões relevantes da interação conjugal. Os resultados mostraram algumas diferenças entre homens e mulheres de ambos os grupos e algumas semelhanças no processo de escolha amorosa dos sujeitos hetero e dos sujeitos homossexuais. Tais resultados são relevantes para a prática clínica da terapia de casal, possibilitando uma melhor compreensão das especificidades da relação amorosa em diferentes tipos de conjugalidade.

  1. A interação na arte contemporânea

    Directory of Open Access Journals (Sweden)

    Ana Claudia de Oliveira

    2008-11-01

    Full Text Available Na arte contemporânea, o sentido se desenrola no presente da situação de exposição da obra, um texto que só se faz enquanto tal com e pelo outro que, interagindo presencialmente, o processa, ou seja, vivência o fazer sentido que faz ocorrer. Enquanto uma forma de vida viví­vel, a investigação interconecta esse regime de sentido como uma das saí­das ou escapatórias, segundo as postulações de A. J. Greimas em De l-Imperfection. Seriam pois as instalações, performances, happenings, land art, obras minimalistas, animações em 3D, sentidos sentidos. Do interacional, a arte, sensivelmente pelas sinestesias que desencadeia, ressignifica a sua própria produção de sentido. Palavras-chave arte contemporânea, sentido, interação, presença, experiência estésica. Abstract In contemporary art, an artistic work, as such, literally cannot exist on its own. It constitutively requires the presence and the cooperation of an observer. In other words, meaning, in such a context,arises only in the very moment of the exhibition of the object, and never previously to the active participation, upon the sensitive level of a receiver. Such an analytical perspective derives from A. J. Greimas-s semiotic postulations about aesthesis experience (in De l-Imperfection, 1987, as well as from E. Landowski-s more recent approach to the notion of presence. Installations, performances, happenings, land art, minimalist artand 3D animations represent privileged terrains in which to study the way contemporary art exposes, on the sensitive level, its own conditions of making sense. Key words contemporary art, sense, interaction, presence, aesthesis experience, meaning.

  2. Imagem corporal em mulheres submetidas à cirurgia bariátrica: Interações socioculturais

    Directory of Open Access Journals (Sweden)

    Marcela Rodrigues de Castro

    2013-10-01

    Full Text Available Apontada como epidemia global a obesidade vem sendo tratada através da cirurgia bariátrica, método invasivo, porém, considerado o mais eficiente. Objetivou-se investigar aspectos socioculturais e seu impacto sobre a imagem corporal de mulheres submetidas à cirurgia bariátrica nos períodos pré e pós-cirúrgico. Foram entrevistadas 20 mulheres submetidas à gastroplastia há pelo menos um ano no Serviço de Controle de Hipertensão Diabetes e Obesidade (SCHDO. Os dados foram analisados sob a perspectiva qualitativa e submetidos à Análise de Conteúdo. A interação entre o contexto sociocultural e imagem corporal configurou-se nas categorias: discriminação, percepção do olhar do outro antes e após cirurgia, e percepção do próprio corpo antes e após cirurgia. Constatou-se que a imagem corporal foi elaborada a partir do meio em que viveu o grupo pesquisado. O meio externo propiciou ao sujeito sentimentos de inadequação corporal, tristeza, exclusão social e inferioridade, resultando em autoconceito negativo. Tais sentimentos foram amenizados após a cirurgia bariátrica proporcionando ao grupo autoconceito positivo decorrente de uma maior inserção social.

  3. Replication of Equine Infectious Anemia Virus in Engineered Mouse NIH 3T3 Cells ▿

    OpenAIRE

    Zhang, Baoshan; Montelaro, Ronald C.

    2008-01-01

    We employed the equine lentivirus equine infectious anemia virus (EIAV) to investigate the cellular restrictions for lentivirus replication in murine NIH 3T3 cells. The results of these studies demonstrate that NIH 3T3 cells expressing the EIAV receptor ELR1 and equine cyclin T1 supported productive replication of EIAV and produced infectious virions at levels similar to those found in a reference permissive equine cell line. The studies presented here demonstrate, for the first time, differe...

  4. High-dose Resveratrol Inhibits Insulin Signaling Pathway in 3T3-L1 Adipocytes

    OpenAIRE

    Lee, Haemi; Kim, Jae-Woo

    2013-01-01

    Background Insulin resistance is a major factor in the development of metabolic syndrome and is associated with central obesity and glucose intolerance. Resveratrol, a polyphenol found in fruits, has been shown to improve metabolic conditions. Although it has been widely studied how resveratrol affects metabolism, little is known about how resveratrol regulates lipogenesis with insulin signaling in 3T3-L1 adipocytes. Methods: We treated differentiated 3T3-L1 adipocytes with resveratrol to obs...

  5. High-dose Resveratrol Inhibits Insulin Signaling Pathway in 3T3-L1 Adipocytes

    OpenAIRE

    Lee, Haemi; Kim, Jae-Woo

    2013-01-01

    Background Insulin resistance is a major factor in the development of metabolic syndrome and is associated with central obesity and glucose intolerance. Resveratrol, a polyphenol found in fruits, has been shown to improve metabolic conditions. Although it has been widely studied how resveratrol affects metabolism, little is known about how resveratrol regulates lipogenesis with insulin signaling in 3T3-L1 adipocytes. Methods: We treated differentiated 3T3-L1 adipocytes with resveratrol to obs...

  6. Uncoupling of 3T3-L1 gene expression from lipid accumulation during adipogenesis

    OpenAIRE

    Temple, Karla A.; Basko, Xheni; Allison, Margaret B.; Brady, Matthew J.

    2007-01-01

    Adipocyte differentiation comprises altered gene expression and increased triglyceride storage. To investigate the interdependency of these two events, 3T3-L1 cells were differentiated in the presence of glucose or pyruvate. All adipocytic proteins examined were similarly increased between the two conditions. In contrast, 3T3-L1 adipocytes differentiated with glucose exhibited significant lipid accumulation, which was largely suppressed in the presence of pyruvate. Subsequent addition of gluc...

  7. A violência rompendo interações: as interações superando a violência Violence rupturing interaction: interaction overcomimg violence

    Directory of Open Access Journals (Sweden)

    Elza Machado de Melo

    2007-03-01

    Full Text Available Trata-se de pesquisa qualitativa associada ao Projeto de Extensão e de Ensino "Frutos do Morro Programa Meninos do Rio", do Departamento de Medicina Preventiva e Social da Faculdade de Medicina da Universidade de Minas Gerais - que se desenvolve em Escolas Públicas do Aglomerado Urbano Morro das Pedras, Belo Horizonte, Minas Gerais, e aborda a percepção de adolescentes acerca da violência, os significados que dão a ela e as formas de enfrentamento geradas. Utilizamos como referencial teórico a tese da colonização do Mundo da Vida, de Jürgen Habermas, em que os imperativos sistêmicos, mediados pelo poder e pelo dinheiro, substituem os laços de sociabilidade e solidariedade próprios das interações coordenadas pela comunicação lingüística. MÉTODOS: foram selecionados, em 2005, 14 grupos focais de adolescentes das Escolas Públicas do Aglomerado, recrutados segundo idade, escola, turno. RESULTADOS: a violência assume papel de mediador das relações intersubjetivas, seja pela falta de opção, pela sedução ou pela presença cotidiana. Embora haja uma naturalização da violência há também um desejo explícito pela sua superação. A relação com o tráfico e o uso de drogas é constantemente mencionada. A violência simbólica - discriminação - é intensamente sentida e vista como causa da violência física. A violência policial é considerada a mais bruta e a mais irracional. Também presente é a violência familiar. CONCLUSÕES: os adolescentes do Morro convivem intimamente com a violência, não vêem muitas alternativas a ela, mas a capacidade de crítica e o desejo de mudança, ainda preservados, representam importantes elementos para a construção de intervenções apropriadas, corroborando a atuação do Projeto Frutos do Morro, fundada na criação de espaços de participação e restabelecimento de laços de solidariedade.This is a qualitative survey associated to the Extension and Education Project

  8. QUANTITATIVE STUDY ON APOPTOSIS INDUCED BY ELECTROMAGNETIC PULSES IN NIH- 3T3 FIBROBLASTS

    Institute of Scientific and Technical Information of China (English)

    ZHAO Mei - lan; CAO Xiao - zhe; WANG De - wen; GU Qing- yang; LIU Jie

    2002-01-01

    Aim: To observe the apoptotic changes following exposure to EMP and to explore the possible injury mechanism in NIH - 3T3 fibroblasts. Methods: Following NIH - 3T3 cells were exposed to EMP,the proliferation and viability of NIH - 3T3 fibroblasts were estimated by hemacytometer and MTT Measurements. Apoptotic rate was measured by flow cytometer. The imnmohistochemical SP method was used to determine bcl- 2, p53. The positively stained cells were analyzed with CMIAS- Ⅱ image analysis system at a magnification 400. All data were analyzed by SPSS8.0 software. Results: The proliferation and viability of NIH- 3T3 cells were markedly inhibited and significant apoptosis was induced following exposure to EMP.EMP could increase the number of non- adherent cells, the highest ratio (33.9%) of non- adherent cells also occurred at 6h. The As70 value of MTT decreased immediately at 1 h, 6h following the cells were exposed as compared with the control (P < 0.05). The highest ratio of apoptosis was 15.07% at 6h, then decreased gradually. Down - regulation of bcl - 2 and up - regulation of p53 were induced by EMP ( P < 0.05). Conclusions: EMP could promote apoptosis of NIH - 3T3 fibroblasts. EMP could also down - regulate bcl - 2 level and up - regulate p53 level in NIH - 3T3 fibroblasts. Bcl - 2 and p53 gene may take part in the process of apoptosis.

  9. CERN Technical Training 2007: IT3T - IT Technical Training Tutorials (Autumn 2007)

    CERN Multimedia

    2007-01-01

    CERN Technical Training and the Internet Services group of the IT department (IT/IS) are jointly organizing a series of free tutorials, addressing some topics of common interest: the IT Technical Training Tutorials (IT3T). The first IT3T series will be offered in October 2007, in French, with the following schedule: IT3T/2007/1 "Introduction to Collaboration Workspaces using SharePoint", October 23rd , 14:30-16:00, Alexandre Lossent IT3T/2007/2 "What is new in Office 2007", October 25th, 14:30-15:30, Emmanuel Ormancey IT3T/2007/3 "Working with Windows Vista at CERN", October 30th, 14:30-15:30, Michal Kwiatek IT3T/2007/4 "Read your mail and more with Outlook 2007", November 1st, 14:30-15:30, Sebastien Dellabella All IT Technical Training Tutorials will take place in the Training Centre Auditorium (building 593, room 11), at 14h30. The tutorials are free of charge, but separate registration to each is required. Participation to any of the tutorials is open: attendance to any ...

  10. CERN Technical Training 2005: IT3T - IT Technical Training Tutorials (Summer 2005)

    CERN Multimedia

    Davide Vitè

    2005-01-01

    CERN Technical Training and the Internet Services group of the IT department (IT/IS) are jointly organizing a series of free tutorials, addressing some topics of common interest: the IT Technical Training Tutorials (IT3T). The first IT3T will be offered in June 2005, in English, with the following schedule: IT3T/1 - Read your mail and more with Outlook 2003: June 14 (Ruben Gaspar) IT3T/2 - Creating, managing and using distribution lists with Simba2: June 16 (Ruben Leivas) IT3T/3 - Working remotely with Windows XP: June 28 (Michel Christaller) IT3T/4 - Editing Websites with FrontPage 2003: June 30 (Andreas Wagner) All IT Technical Training Tutorials will take place in the Training Centre Auditorium (building 593, room 11), from 14h30 to 15h30. The tutorials are free of charge, but separate registration to each is required. Participation to any of the tutorials is open: attendance to any tutorial is not a prerequisite to attend subsequent ones. If you are interested in attending, please discuss with your sup...

  11. Dynamic contrast-enhanced quantitative perfusion measurement of the brain using T-1-weighted MRI at 3T

    DEFF Research Database (Denmark)

    Larsson, H.B.W.; Hansen, A.E.; Berg, H.K.;

    2008-01-01

    Purpose: To develop a method for the measurement of brain perfusion based on dynamic contrast-enhanced T-1-weighted MR imaging. Materials and Methods: Dynamic imaging of the first pass of a bolus of a paramagnetic contrast agent was performed using a 3T whole-body magnet and a T-1-weighted fast...... field echo sequence. The input function was obtained from the internal carotid artery. An initial T-1 measurement was performed in order to convert the MR signal to concentration of the contrast agent. Pixelwise and region of interest (ROI)based calculation of cerebral perfusion (CBF) was performed...... inside the infarct core was, 9 mL/100g/min in one of the stroke patients. The other stroke patient had postischemic hyperperfusion and CBF was 140 mL/100g/min. Conclusion: Absolute values of brain perfusion can be obtained using dynamic contrast-enhanced MRI. These values correspond,to expected values...

  12. Illudins C2 and C3 stimulate lipolysis in 3T3-L1 adipocytes and suppress adipogenesis in 3T3-L1 preadipocytes.

    Science.gov (United States)

    Kim, Sun-Ok; Sakchaisri, Krisada; Asami, Yukihiro; Ryoo, In-Ja; Choo, Soo-Jin; Yoo, Ick-Dong; Soung, Nak-Kyun; Kim, Young Sang; Jang, Jae-Hyuk; Kim, Bo Yeon; Ahn, Jong Seog

    2014-04-25

    The secondary metabolites illudins C2 (1) and C3 (2), obtained from the culture broth of Coprinus atramentarius, have been shown to possess antimicrobial activity. In the present study, we discovered novel biological activities of 1 and 2 in lipolysis of differentiated 3T3-L1 adipocytes and adipogenesis of 3T3-L1 preadipocytes. Compounds 1 and 2 exhibit a dose-dependent increase in glycerol release and thereby reduce intracellular lipid accumulation. The stimulatory effects of 1 and 2 on lipolysis are prevented by cAMP-dependent protein kinase (PKA) and extracellular signal-regulated kinase (ERK) inhibitors. Compounds 1 and 2 down-regulated perilipin and also affected the mRNA and protein levels of adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL). However, 1 and 2 treatment leads to a significant increase in PKA-mediated phosphorylation of HSL at S563 and S660. In addition, 1 and 2 treatment in 3T3-L1 preadipocytes induces down-regulation of the critical transcription factors, CCAAT/enhancer binding protein α and β (C/EBPα and C/EBPβ), and peroxisome proliferator activated receptor γ (PPARγ), which are required for adipogenesis, and accordingly inhibits adipogenesis. These results suggest that 1 and 2 might be useful for treating obesity due to their modulatory effects on fat by affecting adipocyte differentiation and fat mobilization.

  13. A New Promising Technique of 3D Isovoxel Imaging Using 3T MRI in the Wrist: Comparison with 3T MR Arthrography

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Guen Young; Kim, Baek Hyun; Park, Jong Woong [Korea University Ansan Hospital, Korea University College of Medicine, Ansan (Korea, Republic of)

    2011-02-15

    We wanted to evaluate the usefulness of 3D isovoxel MR imaging using 3T MRI in the wrist joint, as compared with 3T MR arthrography. A total of 33 patients underwent both MR arthrography and 3D isovoxel imaging of the wrist joints using 3T MR, including 11 patients with arthroscopic confirmation. 3D isovoxel MR imaging was performed using an intermediateweighted fast spin echo coronal scan with a 0.4-mm slice thickness and the axial images were reconstructed with a 1-mm slice thickness. One radiologist evaluated for the presence of scapholunate or lunotriquetral ligament tear and she determined the grade of the triangular fibrocartilage complex tear and chondromalacia with its location. We compared the two examinations using kappa values. The rates of detecting wrist injury were similar for both exams with substantial to almost perfect inter-examination agreement (kappa value = 0.864 for scapholunate ligament tear, 0.835 for lunotriquetral ligament tear, 0.799 for TFCC tear and 0.940 for chondromalacia). For the eleven cases that underwent arthroscopy, their results of 3D isovoxel MRI were also similar to that of MR arthrography. 3D isovoxel MR imaging is useful for the evaluation of the wrist joint

  14. Prolonged induction activates Cebpα independent adipogenesis in NIH/3T3 cells.

    Directory of Open Access Journals (Sweden)

    Hsiao-Yun Shao

    Full Text Available BACKGROUND: 3T3-L1 cells are widely used to study adipogenesis and insulin response. Their adipogenic potential decreases with time in the culture. Expressing exogenous genes in 3T3-L1 cells can be challenging. This work tries to establish and characterize an alternative model of cultured adipocytes that is easier to work with than the 3T3-L1 cells. METHODOLOGY/PRINCIPAL FINDINGS: INDUCED CELLS WERE IDENTIFIED AS ADIPOCYTES BASED ON THE FOLLOWING THREE CHARACTERISTICS: (1 Accumulation of triglyceride droplets as demonstrated by oil red O stain. (2 Transport rate of 2-deoxyglucose increased after insulin stimulation. (3 Expression of fat specific genes such as Fabp4 (aP2, Slc2a4 (Glut4 and Pparg (PPARγ. Among the cell lines induced under different conditions in this study, only NIH/3T3 cells differentiated into adipocytes after prolonged incubation in 3T3-L1 induction medium containing 20% instead of 10% fetal bovine serum. Rosiglitazone added to the induction medium shortened the incubation period from 14 to 7 days. The PI3K/AKT pathway showed similar changes upon insulin stimulation in these two adipocytes. C/EBPα mRNA was barely detectable in NIH/3T3 adipocytes. NIH/3T3 adipocytes induced in the presence of rosiglitazone showed higher 2-deoxyglucose transport rate after insulin stimulation, expressed less Agt (angiotensinogen and more PPARγ. Knockdown of C/EBPα using shRNA blocked 3T3-L1 but not NIH/3T3 cell differentiation. Mouse adipose tissues from various anatomical locations showed comparable levels of C/EBPα mRNA. CONCLUSIONS/SIGNIFICANCE: NIH/3T3 cells were capable of differentiating into adipocytes without genetic engineering. They were an adipocyte model that did not require the reciprocal activation between C/EBPα and PPARγ to differentiate. Future studies in the C/EBPα independent pathways leading to insulin responsiveness may reveal new targets to diabetes treatment.

  15. Multimodal imaging: Simultaneous EEG in a 3T Hybrid MR–PET system

    Energy Technology Data Exchange (ETDEWEB)

    Neuner, I., E-mail: i.neuner@fz-juelich.de [Institute of Neuroscience and Medicine (INM-4), Forschungszentrum Jülich GmbH (Germany); Department of Psychiatry, Psychotherapy and Psychosomatics, RWTH Aachen University (Germany); JARA BRAIN—Translational Medicine (Germany); Warbrick, T.; Tellmann, L.; Rota Kops, E.; Arrubla, J.; Boers, F.; Herzog, H. [Institute of Neuroscience and Medicine (INM-4), Forschungszentrum Jülich GmbH (Germany); Shah, N.J. [Institute of Neuroscience and Medicine (INM-4), Forschungszentrum Jülich GmbH (Germany); Department of Neurology, RWTH Aachen University (Germany); JARA BRAIN—Translational Medicine (Germany)

    2013-02-21

    The new generation of integrated MR–PET systems allows the simultaneous acquisition of MR and PET data. While MR delivers structural data with an excellent spatial resolution, the advantage of PET is its information on a molecular level. However, both modalities have a low temporal resolution. Thus, for pharmacological studies or patients who suffer from treatment resistant epilepsy the combination of yet another modality such as EEG could be desirable. We tested the feasibility of evoked visual potentials in a 3T Hybrid MR–PET system (Siemens Germany) in comparison to a standalone 3T Trio System (Siemens Germany). A T2⁎-weighted EPI sequence was used: TR: 2.2 s, TE: 30 ms, FOV: 200 mm, slice thickness 3, 36 slices in a healthy volunteer (male, 27 years old) using an MR-compatible 32-channel EEG system (Brainproducts, Munich, Germany). We applied 200 trials of visual stimulation from a white and black checkerboard. Visual evoked potentials were analyzed using Brain Vision Analyzer (Brainproducts, Munich, Germany). Gradient correction and cardioballistic artefact correction were performed as implemented in Vision Analyzer. Visual event related potentials were successfully recorded at the 3T Hybrid MR–PET system. Both curves differ slightly in shape and latency due to the following factors: the distance from the screen varies slightly and the size of the field of view of the subjects is smaller in the 3T MR–PET system in comparison to the 3T stand alone system. Extending the 3T MR–PET Hybrid system to 3T Hybrid MR–PET–EEG is feasible and adds another tool to clinical neuroimaging and research.

  16. Potenciais interações medicamentosas em pacientes com artrite reumatoide Potential drug interactions in patients with rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    Fabíola Bagatini

    2011-02-01

    Full Text Available INTRODUÇÃO: O termo polifarmácia, ou seja, a utilização concomitante de múltiplos fármacos pelo mesmo indivíduo vem sendo amplamente associado a pacientes institucionalizados e idosos, no entanto pode ocorrer em grupos de pacientes portadores de doenças crônicas como artrite reumatoide (AR. OBJETIVO: Quantificar a polifarmácia em um grupo de pacientes com AR e realizar um levantamento sobre o risco de potenciais interações indesejáveis entre os medicamentos utilizados no manejo dessa doença e os fármacos utilizados em enfermidades não crônicas. MÉTODOS: Realizou-se um estudo de coorte com 103 pacientes portadores de AR, atendidos no Componente Especializado da Assistência Farmacêutica/MS, Florianópolis/SC. Os pacientes foram acompanhados mensalmente, por meio de fichas. As interações medicamentosas foram identificadas pelo Drugdex System - Thomson Micromedex® - Interactions. RESULTADOS: Observou-se a presença de polifarmácia em 95,1% dos pacientes e de 19 potenciais interações indesejáveis entre os medicamentos utilizados por 74 pacientes, em média 3,0 ± 1,2 interações/paciente. Todas as potenciais interações estavam relacionadas a metotrexato. Omeprazol foi o principal representante, correspondendo a 29,3% delas, seguido por diclofenaco sódico (17,6% e dipirona sódica (13,2%. CONCLUSÃO: Considerando que este estudo confirma que a polifarmácia é uma prática comum na terapêutica dos pacientes portadores de AR, deve haver maior vigilância acerca de efeitos adversos ou de redução da efetividade de determinados fármacos devido às suas interações farmacológicasINTRODUCTION: The term polypharmacy, meaning the concomitant use of multiple medications by one individual, has been widely reported in institutionalized or elderly patients. It can, however, occur in patients with chronic diseases, such as rheumatoid arthritis (RA. OBJECTIVE: To quantify polypharmacy in a group of RA patients and to assess the

  17. Discussão virtual sobre "interação em epistemologia genética"

    OpenAIRE

    Becker, Fernando; Reis, Rafael dos Reis

    2013-01-01

    O objetivo deste texto é apresentar as ideias e as reflexões que surgiram do Debate sobre o conceito de interação em Epistemologia Genética na Lista de Discussão do GPEGBrasil, entre os meses de Outubro e Dezembro de 2012. A lista congrega, hoje, quase 300 pesquisadores em Epistemologia e Psicologia Genéticas de todo o Brasil. A intenção dos organizadores é registrar, na memória escrita, em forma de uma publicação acadêmica, as contribuições deste Debate para o esclarecimento deste conceito. ...

  18. Polifarmácia: interações e reações adversas no uso de medicamentos por idosos

    OpenAIRE

    Secoli,Silvia Regina

    2010-01-01

    O artigo tem por objetivo refletir sobre a polifarmácia em idosos com ênfase nas reações adversas e nas interações medicamentosas. A vulnerabilidade dos idosos aos problemas decorrentes do uso de medicamentos é bastante alta, o que se deve a complexidade dos problemas clínicos, à necessidade de múltiplos agentes terapêuticos e às alterações farmacocinéticas e farmacodinâmicas inerentes ao envelhecimento. Deste modo, racionalizar o uso de medicamentos e evitar os agravos advindos da polifarmác...

  19. Interação entre cana-de-açúcar e bactérias associadas

    OpenAIRE

    Priscilla de Barros Rossetto

    2008-01-01

    Muitos fatores, como variações sazonais, tipos de tecido vegetal, cultivares e espécies de hospedeiro, tipo de solo, interação com microrganismos benéficos ou patógenos entre outros, afetam a estrutura e a composição da comunidade bacteriana das plantas. A introdução de plantas geneticamente modificadas (PGM) foi somada ao conjunto desses fatores, podendo acarretar efeitos diretos e indiretos sobre a comunidade bacteriana. Cana-de-açúcar é uma cultura de grande importância no Brasil; a área d...

  20. mdm-2 gene amplification in 3T3-L1 preadipocytes.

    Science.gov (United States)

    Berberich, S J; Litteral, V; Mayo, L D; Tabesh, D; Morris, D

    1999-05-01

    In this study the regulation of the murine double minute-2 (mdm-2) gene was examined in NIH 3T3-L1 preadipocytes. The 3T3-L1 cell line, under proper conditions, has the capacity to differentiate from fibroblasts into adipocytes [15]. A recent report demonstrated that mdm-2 overexpression could block myogenesis [12]. While examining the regulation of the mdm-2 gene during adipogenesis, it was discovered that 3T3-L1 cells possess a 36-fold elevation of mdm-2 mRNA relative to A31 cells, another immortalized Balb/c 3T3 fibroblast cell line that lacks the capacity to differentiate. Based on Southern blot analysis, the increase in mdm-2 mRNA was the result of a mdm-2 gene amplification. The level of Mdm-2 protein in undifferentiated 3T3-L1 cells was elevated relative to A31 fibroblasts and resulted from translation of mRNA transcripts initiating from the p53-independent P1 promoter. We also examined how mdm-2 and p53 levels changed as undifferentiated fibroblasts converted to adipocytes. While mdm-2 mRNA levels remained elevated, p53 mRNA, protein, and DNA-binding activity decreased. These results suggest that adipogenesis is unaffected by elevated Mdm-2 levels and that the overexpression of mdm-2 mRNA is predominantly p53 independent.

  1. Protein turnover and cellular autophagy in growing and growth-inhibited 3T3 cells

    Energy Technology Data Exchange (ETDEWEB)

    Papadopoulos, T.; Pfeifer, U. (Univ. of Wuerzburg (West Germany))

    1987-07-01

    The relationship between growth, protein degradation, and cellular autophagy was tested in growing and in growth-inhibited 3T3 cell monolayers. For the biochemical evaluation of DNA and protein metabolism, growth-inhibited 3T3 cell monolayers with high cell density and growing 3T3 cell monolayers with low cell density were labeled simultaneously with ({sup 14}C)thymidine and ({sup 3}H)leucine. The evaluation of the DNA turnover and additional ({sup 3}H)thymidine autoradiography showed that 24 to 5% of 3T3 cells continue to replicate even in the growth-inhibited state, where no accumulation of protein and DNA can be observed. Cell loss, therefore, has to be assumed to compensate for the ongoing cell proliferation. When the data of protein turnover were corrected for cell loss, it was found that the rate constant of protein synthesis in nongrowing monolayers was reduced to half the value found in growing monolayers. Simultaneously, the rate constant of protein degradation in nongrowing monolayers was increased to about 1.5-fold the value of growing monolayers. These data are in agreement with the assumption that cellular autophagy represents a major pathway of regulating protein degradation in 3T3 cells and that the regulation of autophagic protein degradation is of relevance for the transition from a growing to a nongrowing state.

  2. Cytotoxicity of Titanate-Calcium Complexes to MC3T3 Osteoblast-Like Cells

    Directory of Open Access Journals (Sweden)

    Yen-Wei Chen

    2016-01-01

    Full Text Available Monosodium titanates (MST are a relatively novel form of particulate titanium dioxide that have been proposed for biological use as metal sorbents or delivery agents, most recently calcium (II. In these roles, the toxicity of the titanate or its metal complex is crucial to its biological utility. The aim of this study was to determine the cytotoxicity of MST and MST-calcium complexes with MC3T3 osteoblast-like cells; MST-Ca(II complexes could be useful to promote bone formation in various hard tissue applications. MC3T3 cells were exposed to native MST or MST-Ca(II complexes for 24–72 h. A CellTiter-Blue® assay was employed to assess the metabolic activity of the cells. The results showed that MST and MST-Ca(II suppressed MC3T3 metabolic activity significantly in a dose-, time-, and cell-density-dependent fashion. MST-Ca(II suppressed MC3T3 metabolism in a statistically identical manner as native MST at all concentrations. We concluded that MST and MST-Ca(II are significantly cytotoxic to MC3T3 cells through a mechanism yet unknown; this is a potential problem to the biological utility of these complexes.

  3. Design of a loop resonator with a split-ring-resonator (SRR) for a human-body coil in 3 T MRI systems

    Science.gov (United States)

    Son, Hyeok Woo; Cho, Young Ki; Kim, Byung Mun; Back, Hyun Man; Yoo, Hyoungsuk

    2016-04-01

    A new radio-frequency (RF) resonator for Nuclear Magnetic Resonance (NMR) imaging at clinical magnetic resonance imaging (MRI) systems is proposed in this paper. An approach based on the effects of the properties of metamaterials in split-ring resonators (SRRs) is used to design a new loop resonator with a SRR for NMR imaging. This loop resonator with a SRR is designed for NMR imaging at 3 T MRI systems. The 3D electromagnetic simulation was used to optimize the design of the proposed RF resonator and analyze it's performance at 3 T MRI systems. The proposed RF resonator provides strong penetrating magnetic fields at the center of the human phantom model, approximately 10%, as compared to the traditional loop-type RF resonator used for NMR imaging at clinical MRI systems. We also designed an 8-channel body coil for human-body NMR imaging by using the proposed loop resonator with a SRR. This body coil also produces more homogeneous and highly penetrating magnetic fields into the human phantom model.

  4. Interação de glyphosate com carfentrazone-ethyl Glyphosate - carfentrazone-ethyl interaction

    Directory of Open Access Journals (Sweden)

    R.C. Werlang

    2002-04-01

    Full Text Available Foi conduzido um experimento em condições controladas para determinar a interação do carfentrazone-ethyl em mistura no tanque com o herbicida glyphosate, no controle de seis espécies de plantas daninhas. Glyphosate aplicado isoladamente na dose de 720 g ha-1 foi eficaz no controle de Amaranthus hybridus (100%, Desmodium tortuosum (100%, Bidens pilosa (99%, Eleusine indica (96%, Digitaria horizontalis (100% e Commelina benghalensis (93% aos 21 DAA. Carfentrazone-ethyl aplicado isoladamente controlou eficazmente C. benghalensis. As misturas de glyphosate nas doses de 252 e 720 g ha-1 com carfentrazone-ethyl nas doses de 15 e 30 g ha¹ demonstraram efeito aditivo no controle de A. hybridus, D. tortuosum e Bidens pilosa, à exceção das misturas de glyphosate na dose de 252 g ha-1 com as doses de 15 e 30 g ha-1 de carfentrazone-ethyl, que proporcionam efeito sinergístico no controle de D. tortuosum. A adição das duas doses de carfentrazone-ethyl antagonizou o efeito de glyphosate na menor dose (252 g ha-1 no controle de E. indica, apresentando, no entanto, efeito aditivo com o glyphosate na maior dose (720 g ha-1. Já para D. horizontalis, as misturas de carfentrazone-ethyl com glyphosate na menor dose (252 g ha-1 apresentaram efeito sinergístico no controle dessa espécie, demonstrando, ainda, efeito aditivo na mistura com glyphosate na dose de 720 g ha-1. A mistura de carfentrazone-ethyl com glyphosate proporcionou efeito aditivo no controle de C. benghalensis, independentemente das combinações de doses avaliadas. Os resultados deste experimento indicam que carfentrazone-ethyl apresenta comportamento diferenciado quanto à interação com glyphosate, dependendo da espécie de planta daninha e da dose dos herbicidas utilizados na mistura em tanque, sendo complementar na mistura em tanque com glyphosate, pois demonstrou efeito antagônico em poucas das combinações estudadas, prevalecendo seu efeito aditivo na mistura com glyphosate, no

  5. Calcification of MC3T3-E1 cells on titanium and zirconium.

    Science.gov (United States)

    Umezawa, Takayuki; Chen, Peng; Tsutsumi, Yusuke; Doi, Hisashi; Ashida, Maki; Suzuki, Shoichi; Moriyama, Keiji; Hanawa, Takao

    2015-01-01

    To confirm similarity of hard tissue compatibility between titanium and zirconium, calcification of MC3T3-E1 cells on titanium and zirconium was evaluated in this study. Mirror-polished titanium (Ti) and zirconium (Zr) disks and zirconium-sputter deposited titanium (Zr/Ti) were employed in this study. The surface of specimens were characterized using scanning electron microscopy and X-ray diffraction. Then, the cellular proliferation, differentiation and calcification of MC3T3-E1 cells on specimens were investigated. The surface of Zr/Ti was much smoother and cleaner than those of Ti and Zr. The proliferation of the cell was the same among three specimens, while the differentiation and calcification on Zr/Ti were faster than those on Ti and Zr. Therefore, Ti and Zr showed the identical hard tissue compatibility according to the evaluation with MC3T3-E1 cells. Sputter deposition may improve cytocompatibility.

  6. On (2, 3, t-Generations for the Conway Group Co2

    Directory of Open Access Journals (Sweden)

    Jintanaporn Wattanathorn

    2012-01-01

    Full Text Available Problem statement: In this article we investigate all the (2, 3, t-generations for the Conway’s second largest sporadic simple group Co2, where t is an odd divisor of order of Co2. Approach: An (l, m, n-generated group G is a quotient group of the triangle group T (l, m, n = (x, y, z|xl = ym = zn = xyz = 1. A group G is said to be (2, 3, t-generated if it can be generated by two elements x and y such that o(x = 2, o(y = 3 and o (xy = t. Computations are carried out with the aid of computer algebra system GAP-Groups, Algorithms and Programming. Results and Conclusion: The Conway group Co2 is (2, 3, t-generated for t an odd divisor of order of Co2 except when t = 5, 7, 9.

  7. Advances in hybrid MR–PET at 3 T and 9.4 T in humans

    Energy Technology Data Exchange (ETDEWEB)

    Jon Shah, N., E-mail: n.j.shah@fz-juelich.de [Institute of Neuroscience and Medicine-4, Research Centre Jülich, 52425 Jülich (Germany); Department of Neurology, Faculty of Medicine, JARA, RWTH Aachen University Aachen (Germany); Mauler, Jörg [Institute of Neuroscience and Medicine-4, Research Centre Jülich, 52425 Jülich (Germany); Neuner, Irene [Institute of Neuroscience and Medicine-4, Research Centre Jülich, 52425 Jülich (Germany); Department of Psychiatry, Psychotherapy and Psychosomatics, RWTH Aachen University, Aachen (Germany); Oros-Peusquens, Ana-Maria; Romanzetti, Sandro; Vahedipour, Kaveh; Felder, Jörg; Celik, Avdo [Institute of Neuroscience and Medicine-4, Research Centre Jülich, 52425 Jülich (Germany); Iida, Hidehiro [Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, 5-7-1, Fujishirodai, Suita, Osaka, 565-8565 (Japan); Langen, Karl-Josef; Herzog, Hans [Institute of Neuroscience and Medicine-4, Research Centre Jülich, 52425 Jülich (Germany)

    2013-02-21

    Hybrid MR–PET data acquisition in simultaneous mode confers a number of advantages at 3 T and 9.4 T. From an MR perspective, the potential for ultra-high resolution structural imaging is discussed and example images of the cerebellum with an isotropic resolution of 320 μm are presented. Further, metabolic imaging is discussed and high-resolution images of the sodium distribution are demonstrated. Examples of tumour imaging on a 3 T MR–PET system are included and discussed.

  8. Uncoupling of 3T3-L1 gene expression from lipid accumulation during adipogenesis.

    Science.gov (United States)

    Temple, Karla A; Basko, Xheni; Allison, Margaret B; Brady, Matthew J

    2007-02-06

    Adipocyte differentiation comprises altered gene expression and increased triglyceride storage. To investigate the interdependency of these two events, 3T3-L1 cells were differentiated in the presence of glucose or pyruvate. All adipocytic proteins examined were similarly increased between the two conditions. In contrast, 3T3-L1 adipocytes differentiated with glucose exhibited significant lipid accumulation, which was largely suppressed in the presence of pyruvate. Subsequent addition of glucose to the latter cells restored lipid accumulation and acute rates of insulin-stimulated lipogenesis. These data indicate that extracellular energy is required for induction of adipocytic proteins, while only glucose sustained the parallel increase in triglyceride storage.

  9. (2,3,t)-generations for the Harada-Norton group HN

    Science.gov (United States)

    Ibrahim, Mohammed Ali Faya

    2013-09-01

    A group G is said to be (2,3)-generated if it can be generated by two of elements x and y such that o(x) = 2, o(y) = 3. Further if o(xy) = t we say that the group G is (2,3,t)-generated. In this article, we determine all (2,3,t)-generations for the Harada-Norton sporadic simple group HN, where t is any odd divisor of the order of HN.

  10. In vivo imaging of human breast cancer mouse model with high level expression of calcium sensing receptor at 3T.

    Science.gov (United States)

    Baio, Gabriella; Fabbi, Marina; Emionite, Laura; Cilli, Michele; Salvi, Sandra; Ghedin, Piero; Prato, Sabina; Carbotti, Grazia; Tagliafico, Alberto; Truini, Mauro; Neumaier, Carlo Emanuele

    2012-03-01

    To demonstrate that manganese can visualise calcium sensing receptor (CaSR)-expressing cells in a human breast cancer murine model, as assessed by clinical 3T magnetic resonance (MR). Human MDA-MB-231-Luc or MCF7-Luc breast cancer cells were orthotopically grown in NOD/SCID mice to a minimum mass of 5 mm. Mice were evaluated on T1-weighted sequences before and after intravenous injection of MnCl(2). To block the CaSR-activated Ca(2+) channels, verapamil was injected at the tumour site 5 min before Mn(2+) administration. CaSR expression in vivo was studied by immunohistochemistry. Contrast enhancement was observed at the tumour periphery 10 min after Mn(2+) administration, and further increased up to 40 min. In verapamil-treated mice, no contrast enhancement was observed. CaSR was strongly expressed at the tumour periphery. Manganese enhanced magnetic resonance imaging can visualise CaSR-expressing breast cancer cells in vivo, opening up possibilities for a new MR contrast agent. • Manganese contrast agents helped demonstrate breast cancer cells in an animal model. • Enhancement was most marked in cells with high calcium sensing receptor expression. • Manganese uptake was related to the distribution of CaSR within the tumour. • Manganese MRI may become useful to investigate human breast cancer.

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  5. File list: DNS.EmF.20.AllAg.NIHSLASH3T3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.EmF.20.AllAg.NIHSLASH3T3 mm9 DNase-seq Embryonic fibroblast NIH/3T3 SRX188658,S...RX716376,SRX191035,SRX1054382 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.EmF.20.AllAg.NIHSLASH3T3.bed ...

  6. File list: Unc.EmF.05.AllAg.NIHSLASH3T3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.EmF.05.AllAg.NIHSLASH3T3 mm9 Unclassified Embryonic fibroblast NIH/3T3 SRX62030...3,SRX620305,SRX620302,SRX620304 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.EmF.05.AllAg.NIHSLASH3T3.bed ...

  7. File list: DNS.EmF.10.AllAg.NIHSLASH3T3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.EmF.10.AllAg.NIHSLASH3T3 mm9 DNase-seq Embryonic fibroblast NIH/3T3 SRX188658,S...RX191035,SRX716376,SRX1054382 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/DNS.EmF.10.AllAg.NIHSLASH3T3.bed ...

  8. File list: Unc.EmF.20.AllAg.NIHSLASH3T3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Unc.EmF.20.AllAg.NIHSLASH3T3 mm9 Unclassified Embryonic fibroblast NIH/3T3 SRX62030...2,SRX620303,SRX620304,SRX620305 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Unc.EmF.20.AllAg.NIHSLASH3T3.bed ...

  9. File list: ALL.Oth.50.AllAg.3T3_fibroblasts [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Oth.50.AllAg.3T3_fibroblasts mm9 All antigens Others 3T3 fibroblasts SRX099255,...SRX105582,SRX099259,SRX099260,SRX099261,SRX099262 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.Oth.50.AllAg.3T3_fibroblasts.bed ...

  10. Avaliação qualitativa de interações em redes sociais: relacionamentos no blog Martelada

    Directory of Open Access Journals (Sweden)

    Alex Primo

    2008-09-01

    Full Text Available Este artigo busca demonstrar a diversidade de interagentes com quem um mesmo blogueiro interage e os diferentes relacionamentos mantidos. Pelo procedimento metodológico qualitativo utilizado (Primo 2006 na investigação empírica do blog Martelada, procura-se discutir as dicotomias ego/alter e laços fracos/laços fortes. A pesquisa visa observar não o aspecto de conteúdo, o que é dito, ou meramente as questões tecnológicas envolvidas, mas sim o aspecto relacional das interações. Palavras-chave: Interação mediada por computador; análise de redes sociais; blog; conversação. ABSTRACT This article aims to show the diversity of interagents with whom any given blogger interacts and the different relationships that are established in this context. Through qualitative methodological procedures used in the empirical investigation of the blog Martelada (Primo 2006, we try to discuss the ego/alter and the strong bonds/weak bonds dichotomies. This piece of research aims to analyse not what is said – the content – or purely technological questions involved, but to focus on the relational aspect of these interactions. Keywords: Computer-mediated interaction; analysis of social networks; blog; conversation.

  11. A epistemologia genética e a interação social de crianças

    Directory of Open Access Journals (Sweden)

    Moro Maria Lucia Faria

    2000-01-01

    Full Text Available O artigo apresenta um estudo de caso sobre as relações da interação social de crianças com suas construções cognitivas individuais de aprendizagem da adição/subtração, segundo proposições da epistemologia genética. Os resultados vêm da análise qualitativa microgenética da seqüência completa, gravada em vídeo, dos eventos referentes às inter-relações: a das estratégias cognitivas expressas por três meninos que, juntos, resolveram tarefas relativas ao conteúdo aritmético citado; b daquelas estratégias com as intervenções do adulto. Os sujeitos (idades de 7,4; 8,6; e 9,1 eram alunos de primeira série de uma escola pública. Os resultados apoiam parcialmente hipóteses sobre um modelo cíclico complexo de inter-relações das realizações dos parceiros e destas com as intervenções do adulto. O modelo piagetiano da equilibração explica a complexidade das interações sociais discutidas, em suas relações necessárias mas não suficientes com as construções cognitivas individuais.

  12. A retrospective comparison of propofol alone to propofol in combination with dexmedetomidine for pediatric 3T MRI sedation.

    Science.gov (United States)

    Boriosi, Juan P; Eickhoff, Jens C; Klein, Kristi B; Hollman, Gregory A

    2017-01-01

    Both propofol and dexmedetomidine have been found to be safe and effective sedation for magnetic resonance imaging (MRI). Our program experienced an increase in patients arousing and experiencing an adverse airway event during propofol sedation for MRI in the first months of using a new 3T (Tesla) MRI scanner that was found to have a longer reverberation time compared to the previous 1.5 T MRI. In an effort to decrease patient arousal and adverse airway events during MRI, we administered a dexmedetomidine load prior to our standard propofol protocol. The objective was to compare adverse events and other outcome measures of patients sedated with propofol alone (Pro) and propofol preceded by a dexmedetomidine load (D+P). We reviewed a sedation database and medical records for all children undergoing 3T MRI studies while sedated with propofol alone or propofol preceded by a dexmedetomidine load in 2014. Two hundred and fifty-six sedations were performed for MRI (87 Pro and 169 D+P). The two groups were comparable with regard to age, weight, gender, and American Society of Anesthesiologists status. Subjects in the D+P cohort had significantly fewer adverse events (10/169 patients (5.9%) vs 23/87 patients (26.4%) [OR 0.18, 95% CI: 0.08-0.39, P propofol MRI sedation protocol resulted in fewer sedation-related adverse events, particularly upper airway obstruction. Further studies are needed to evaluate the potential for a reduction on adverse events with this drug combination. © 2016 John Wiley & Sons Ltd.

  13. SU-E-J-209: Geometric Distortion at 3T in a Commercial 4D MRI-Compatible Phantom

    Energy Technology Data Exchange (ETDEWEB)

    Fatemi-Ardekani, A; Wronski, M; Kim, A; Stanisz, G; Sarfehnia, A; Keller, B [Sunnybrook Health Sciences Centre, Toronto, ON (Canada)

    2015-06-15

    Purpose: There are very few commercial 4D phantoms that are marketed as MRI compatible. We are evaluating one such commercial phantom, made to be used with an MRI-Linear accelerator. The focus of this work is to characterize the geometric distortions produced in this phantom at 3T using 3 clinical MR pulse sequences. Methods: The CIRS MRI-Linac Dynamic Phantom (CIRSTM) under investigation in this study consists of a softwaredriven moving tumour volume within a thorax phantom body and enables dose accumulation by placing a dosimeter within the tumour volume. Our initial investigation is to evaluate the phantom in static mode prior to examining its 4D capability. The water-filled thorax phantom was scanned using a wide-bore Philips 3T Achieva MRI scanner employing a Thoracic xl coil and clinical 2D T1W FFE, 2D T1W TSE and 3D T1W TFE pulse sequences. Each of the MR image sets was rigidly fused with a reference CT image of the phantom employing a rigid registration with 6 degrees of freedom. Geometric distortions between the MR and CT image sets were measured in 3 dimensions at selected points along the periphery of the distortion grid embedded within the phantom body (11.5, 7.5 and 3 cm laterally, ant/post and sup/inf of magnetic isocenter respectively). Results: The maximal measured geometric distortions between the MR and reference CT points of interest were 0.9, 1.8 and 1.3 mm in the lateral, anteriorposterior and cranio-caudal directions, respectively. For all 3 spatial dimensions, the maximal distortions occurred for the FFE pulse sequence. Maximal distortions for the 2D FFE, 2D TSE and 3D TFE sequences were 1, 0.7 and 1.8 mm, respectively. Conclusion: Our initial static investigation of this phantom shows minimal geometric distortions at 3T along the periphery of the embedded grid. CIRS has provided us with a phantom at no charge for evaluation at 3 Tesla.

  14. 49 CFR 178.45 - Specification 3T seamless steel cylinder.

    Science.gov (United States)

    2010-10-01

    ...) Type, size, and service pressure. A DOT 3T cylinder is a seamless steel cylinder with a minimum water... eliminate these defects provided the minimum wall thickness is maintained. (5) Welding or brazing on a cylinder is not permitted. (d) Wall thickness. The minimum wall thickness must be such that the wall...

  15. Methylglyoxal induces oxidative stress and mitochondrial dysfunction in osteoblastic MC3T3-E1 cells.

    Science.gov (United States)

    Suh, K S; Choi, E M; Rhee, S Y; Kim, Y S

    2014-02-01

    Methylglyoxal is a reactive dicarbonyl compound produced by glycolytic processing and identified as a precursor of advanced glycation end products. The elevated methylglyoxal levels in patients with diabetes are believed to contribute to diabetic complications, including bone defects. The objective of this study was to evaluate the effect of methylglyoxal on the function of osteoblastic MC3T3-E1 cells. The data indicated that methylglyoxal decreased osteoblast differentiation and induced osteoblast cytotoxicity. Pretreatment of MC3T3-E1 cells with aminoguanidine (a carbonyl scavenger), Trolox (an antioxidant), and cyclosporin A (a blocker of the mitochondrial permeability transition pore) prevented methylglyoxal-induced cytotoxicity in MC3T3-E1 cells. However, BAPTA/AM (an intracellular Ca(2+) chelator) and dantrolene (an inhibitor of endoplasmic reticulum Ca(2+) release) did not reverse the cytotoxic effect of methylglyoxal. Methylglyoxal increased the formation of intracellular reactive oxygen species, mitochondrial superoxide, and cardiolipin peroxidation in osteoblastic MC3T3-E1 cells. Methylglyoxal also decreased the mitochondrial membrane potential and intracellular ATP and nitric oxide levels, suggesting that carbonyl stress-induced loss of mitochondrial integrity contributes to the cytotoxicity of methylglyoxal. Furthermore, the results demonstrated that methylglyoxal induced protein adduct formation, inactivation of glyoxalase I, and activation of glyoxalase II. Aminoguanidine reversed all aforementioned effects of methylglyoxal. Taken together, these data support the notion that high methylglyoxal concentrations have detrimental effects on osteoblasts through a mechanism involving oxidative stress and mitochondrial dysfunction.

  16. Quantitative PET imaging with the 3T MR-BrainPET

    Energy Technology Data Exchange (ETDEWEB)

    Weirich, C., E-mail: c.weirich@fz-juelich.de [Forschungszentrum Jülich, Institute of Neuroscience and Medicine – 4, Juelich (Germany); Scheins, J.; Lohmann, P.; Tellmann, L. [Forschungszentrum Jülich, Institute of Neuroscience and Medicine – 4, Juelich (Germany); Byars, L.; Michel, C. [Siemens Healthcare, Molecular Imaging, Knoxville, TN (United States); Rota Kops, E.; Brenner, D.; Herzog, H.; Shah, N.J. [Forschungszentrum Jülich, Institute of Neuroscience and Medicine – 4, Juelich (Germany)

    2013-02-21

    The new hybrid imaging technology of MR-PET allows for simultaneous acquisition of versatile MRI contrasts and the quantitative metabolic imaging with PET. In order to achieve the quantification of PET images with minimal residual error the application of several corrections is crucial. In this work we present our results on quantification with the 3T MR BrainPET scanner.

  17. Increased Association of Dynamin Ⅱ with Myosin Ⅱ in Ras Transformed NIH3T3 Cells

    Institute of Scientific and Technical Information of China (English)

    Soon-Jeong JEONG; Su-Gwan KIM; Jiyun YOO; Mi-Young HAN; Joo-Cheol PARK; Heung-Joong KIM; Seong Soo KANG; Baik-Dong CHOI; Moon-Jin JEONG

    2006-01-01

    Dynamin has been implicated in the formation of nascent vesicles through both endocytic and secretory pathways. However, dynamin has recently been implicated in altering the cell membrane shape during cell migration associated with cytoskeleton-related proteins. Myosin Ⅱ has been implicated in maintaining cell morphology and in cellular movement. Therefore, reciprocal immunoprecipitation was carried out to identify the potential relationship between dynamin Ⅱ and myosin Ⅱ. The dynamin Ⅱ expression level was higher when co-expressed with myosin Ⅱ in Ras transformed NIH3T3 cells than in normal NIH3T3 cells.Confocal microscopy also confirmed the interaction between these two proteins. Interestingly, exposing the NIH3T3 cells to platelet-derived growth factor altered the interaction and localization of these two proteins.The platelet-derived growth factor treatment induced lamellipodia and cell migration, and dynamin Ⅱ interacted with myosin Ⅱ. Grb2, a 24 kDa adaptor protein and an essential element of the Ras signaling pathway,was found to be associated with dynamin Ⅱ and myosin Ⅱ gene expression in the Ras transformed NIH3T3 cells. These results suggest that dynamin Ⅱ acts as an intermediate messenger in the Ras signal transduction pathway leading to membrane ruffling and cell migration.

  18. CERN Technical Training 2005: IT3T - IT Technical Training Tutorials (Summer 2005)

    CERN Multimedia

    Davide Vitè

    2005-01-01

    CERN Technical Training and the Internet Services group of the IT department (IT/IS) are jointly organizing a series of free tutorials, addressing some topics of common interest: the IT Technical Training Tutorials (IT3T). The next IT3T will be offered, in English, with the following schedule: IT3T/3 - Working remotely with Windows XP: June 28 (Michel Christaller) IT3T/4 - Editing Websites with FrontPage 2003: June 30 (Andreas Wagner) The IT Technical Training Tutorials will take place in the Training Centre Auditorium (building 593, room 11), from 14h30 to 15h30. The tutorials are free of charge, but separate registration to each is required. Participation to any of the tutorials is open: attendance to any tutorial is not a prerequisite to attend subsequent ones. If you are interested in attending, please discuss with your supervisor and/or your DTO, and apply electronically via EDH. Registered participants will be invited to each tutorial; attendance will be recorded in the personal training records. Mo...

  19. CERN Technical Training 2005: IT3T - IT Technical Training Tutorials (Summer 2005)

    CERN Multimedia

    Davide Vitè

    2005-01-01

    CERN Technical Training and the Internet Services group of the IT department (IT/IS) are jointly organizing a series of free tutorials, addressing some topics of common interest: the IT Technical Training Tutorials (IT3T). The next IT3T will be offered, in English, with the following schedule: IT3T/3 - Working remotely with Windows XP: June 28 (Michel Christaller) IT3T/4 - Editing Websites with FrontPage 2003: June 30 (Andreas Wagner) The IT Technical Training Tutorials will take place in the Training Centre Auditorium (building 593, room 11), from 14:30 to 15:30. The tutorials are free of charge, but separate registration to each is required. Participation to any of the tutorials is open: attendance to any tutorial is not a prerequisite to attend subsequent ones. If you are interested in attending, please discuss with your supervisor and/or your DTO, and apply electronically via EDH. Registered participants will be invited to each tutorial; attendance will be recorded in the personal training records. Mo...

  20. 11 beta-hydroxysteroid dehydrogenase type 1 promotes differentiation of 3T3-L1 preadipocyte

    Institute of Scientific and Technical Information of China (English)

    Yun LIU; Yan SUN; Ting ZHU; Yu XIE; Jing YU; Wen-lan SUN; Guo-xian DING; Gang HU

    2007-01-01

    Aim: To investigate the relationship between 11 beta-hydroxysteroid dehydroge-nase type 1 (1 lbeta-HSD1), a potential link between obesity and type 2 diabetes,and preadipocyte differentiation. Methods: Mouse 11beta-HSD1 siRNA plasmids were transfected into 3T3-L1 preadipocytes (a cell line derived from mouse Swiss3T3 cells that were isolated from mouse embryo), for examination of the effect of targeted 11 beta-HSD1 inhibition on differentiation of 3T3-L1 cells. Dif-ferentiation was stimulated with 3-isobutyl-1-methyxanthine, insulin, and dexamethasone. The transcription level of the genes was detected by real-time PCR. Results: Lipid accumulation was significantly inhibited in cells transfected with mouse 11beta-HSD1 siRNA compared with non-transfected 3T3-L1 cells.Fewer lipid droplets were detected in the transfected cells both prior to stimulation and after stimulation with differentiation-inducing reagents. The expression of adipocyte differentiation-associated markers such as lipoprotein lipase and fatty acid synthetase were downregulated in the transfected cells. Similarly, the expres-sion of preadipocyte factor-1, an inhibitor of adipocyte differentiation, was downregulated upon stimulation of differentiation and had no changes in the transfected cells. Conclusion: 11 beta-HSD1 can promote preadipocyte differentiation. Based on this, we propose that 11 beta-HSD1 may be an important candidate mediator of obesity and obesity-induced insulin resistance.

  1. High-resolution MR imaging of the knee at 3 T

    Energy Technology Data Exchange (ETDEWEB)

    Niitsu, M.; Nakai, T.; Ikeda, K.; Tang, G.Y.; Yoshioka, H.; Itai, Y. [Tsukuba Univ., Ibaraki (Japan). Dept. of Radiology

    2000-07-01

    In order to examine the practical feasibility of using a 3.0-T MR unit to obtain high-quality, high-resolution images of the knee joint, one human cadaveric and 5 porcine knees were imaged with the 3.0-T unit. Sets of T1-weighted spin echo images were obtained with in-plane resolution of 0.195x0.39 mm and an acquisition time of approximately 5 min. Two porcine knees were also imaged with the 1.0-T unit with an identical imaging protocol and the signal-to-noise (S/N) ratios were measured on images at 3 T and 1 T. The 3-T MR system provided detailed delineation of the knees. Deep layers of the medial collateral ligament and associated fine fibers beneath the medial and lateral collateral ligament were demarcated. We observed precise demonstration of the tibial attachment of the anterior cruciate ligament, irregularity of the meniscal free edge, and conjoint tendon formation together with the lateral collateral ligament and the biceps femoris tendon. Compared to the 1-T unit, the S/N ratio with the 3-T unit was increased by a factor of 1.39 to 1.72. Due to the potential advantage of obtaining detailed images, the 3-T MR system suggests a practical utility for fine demonstration of the knee morphology.

  2. 26 CFR 1.702-3T - 4-Year spread (temporary).

    Science.gov (United States)

    2010-04-01

    ... with or within which such short year ends. Example 9. Assume that P3 is a partnership with a taxable... (CONTINUED) INCOME TAXES Partners and Partnerships § 1.702-3T 4-Year spread (temporary). (a) Applicability. This section applies to a partner in a partnership if— (1) The partnership is required by section...

  3. CERN Technical Training 2007: IT3T - IT Technical Training Tutorials (Autumn 2007)

    CERN Multimedia

    2008-01-01

    Videos now available The videos of the first IT3T series, which was offered in French in October 2007 (Office 2007, Sharepoint collaboration workspace, Vista, Outlook 2007), are now available via Indico at: http://indico.cern.ch/categoryDisplay.py?categId=590Enjoy!

  4. CERN Technical Training 2007: IT3T - IT Technical Training Tutorials (Autumn 2007)

    CERN Multimedia

    2008-01-01

    Videos now available The videos of the first IT3T series which was offered in French in October 2007 (office 2007, Sharepoint collaboration workspace, Vista, Outlook 2007), are now available via Indico at the address: http://indico.cern.ch/categoryDisplay.py?categId=590Enjoy!

  5. CERN Technical Training 2007: IT3T - IT Technical Training Tutorials (Autumn 2007)

    CERN Multimedia

    2008-01-01

    Videos now available The videos of the first IT3T series which was offered in French, in October 2007 (office 2007, Sharepoint collaboration workspace, Vista, Outlook 2007), are now available via Indico at the address: http://indico.cern.ch/categoryDisplay.py?categId=590Enjoy!

  6. Isoproterenol Increases Uncoupling, Glycolysis, and Markers of Beiging in Mature 3T3-L1 Adipocytes.

    Science.gov (United States)

    Miller, Colette N; Yang, Jeong-Yeh; England, Emily; Yin, Amelia; Baile, Clifton A; Rayalam, Srujana

    2015-01-01

    Beta-adrenergic activation stimulates uncoupling protein 1 (UCP1), enhancing metabolic rate. In vitro, most work has studied brown adipocytes, however, few have investigated more established adipocyte lines such as the murine 3T3-L1 line. To assess the effect of beta-adrenergic activation, mature 3T3-L1s were treated for 6 or 48 hours with or without isoproterenol (10 and 100 μM) following standard differentiation supplemented with thyroid hormone (T3; 1 nM). The highest dose of isoproterenol increased lipid content following 48 hours of treatment. This concentration enhanced UCP1 mRNA and protein expression. The increase in UCP1 following 48 hours of isoproterenol increased oxygen consumption rate. Further, coupling efficiency of the electron transport chain was disturbed and an enhancement of glycolytic rate was measured alongside this, indicating an attempt to meet the energy demands of the cell. Lastly, markers of beige adipocytes (protein content of CD137 and gene transcript of CITED1) were also found to be upregulated at 48 hours of isoproterenol treatment. This data indicates that mature 3T3-L1 adipocytes are responsive to isoproterenol and induce UCP1 expression and activity. Further, this finding provides a model for further pharmaceutical and nutraceutical investigation of UCP1 in 3T3-L1s.

  7. Isoproterenol Increases Uncoupling, Glycolysis, and Markers of Beiging in Mature 3T3-L1 Adipocytes.

    Directory of Open Access Journals (Sweden)

    Colette N Miller

    Full Text Available Beta-adrenergic activation stimulates uncoupling protein 1 (UCP1, enhancing metabolic rate. In vitro, most work has studied brown adipocytes, however, few have investigated more established adipocyte lines such as the murine 3T3-L1 line. To assess the effect of beta-adrenergic activation, mature 3T3-L1s were treated for 6 or 48 hours with or without isoproterenol (10 and 100 μM following standard differentiation supplemented with thyroid hormone (T3; 1 nM. The highest dose of isoproterenol increased lipid content following 48 hours of treatment. This concentration enhanced UCP1 mRNA and protein expression. The increase in UCP1 following 48 hours of isoproterenol increased oxygen consumption rate. Further, coupling efficiency of the electron transport chain was disturbed and an enhancement of glycolytic rate was measured alongside this, indicating an attempt to meet the energy demands of the cell. Lastly, markers of beige adipocytes (protein content of CD137 and gene transcript of CITED1 were also found to be upregulated at 48 hours of isoproterenol treatment. This data indicates that mature 3T3-L1 adipocytes are responsive to isoproterenol and induce UCP1 expression and activity. Further, this finding provides a model for further pharmaceutical and nutraceutical investigation of UCP1 in 3T3-L1s.

  8. Octanoate and decanoate induce apoptosis in 3T3-L1 adipocytes.

    Science.gov (United States)

    Yang, Jeong-Yeh; Rayalam, Srujana; Della-Fera, Mary Anne; Ambati, Suresh; Baile, Clifton A

    2009-10-01

    The effect of octanoate and decanoate, respectively, eight- and 10-carbon medium-chain fatty acids (MCFAs), on apoptotic signaling in 3T3-L1 adipocytes was investigated. 3T3-L1 adipocytes were treated with various concentrations of octanoate or decanoate. Cell viability, apoptosis, and expression of apoptosis-related proteins were investigated. Results indicated that both octanoate and decanoate decreased viability, increased apoptosis, and increased reactive oxygen species production. Immunoblotting analysis showed an increase in the levels of cytoplasmic cytochrome c and cleaved poly(ADP-ribose) polymerase by octanoate and decanoate. Concomitantly, we observed that pro-caspase-3 was decreased, resulting in the induced accumulation of the cleaved form of caspase-3 by both octanoate and decanoate. In addition, both octanoate and decanoate increased the expression of pro-apoptotic Bax with an accompanied decrease of anti-apoptotic Bcl-2. These results show that octanoate and decanoate mediate adipocyte apoptosis via a caspase-dependent mitochondrial pathway in 3T3-L1 adipocytes. MCFAs thus decrease adipocyte number by initiating the apoptotic process in 3T3-L1 adipocytes.

  9. 26 CFR 1.883-3T - Treatment of controlled foreign corporations (temporary).

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 9 2010-04-01 2010-04-01 false Treatment of controlled foreign corporations... (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Foreign Corporations § 1.883-3T Treatment of controlled foreign corporations (temporary). (a) General rule. A foreign corporation satisfies the stock...

  10. Fluorescence lifetime imaging of lipids during 3T3-L1 cell differentiation

    Science.gov (United States)

    Song, Young Sik; Won, Young Jae; Lee, Sang-Hak; Kim, Dug Young

    2014-03-01

    Obesity is becoming a big health problem in these days. Since increased body weight is due to increased number and size of the triglyceride-storing adipocytes, many researchers are working on differentiation conditions and processes of adipocytes. Adipocytes also work as regulators of whole-body energy homeostasis by secreting several proteins that regulate processes as diverse as haemostasis, blood pressure, immune function, angiogenesis and energy balance. 3T3-L1 cells are widely used cell line for studying adipogenesis because it can differentiate into an adipocyte-like phenotype under appropriate conditions. In this paper, we propose an effective fluorescence lifetime imaging technique which can easily distinguish lipids in membrane and those in lipid droplets. Nile red dyes are attached to lipids in 3T3-L1 cells. Fluorescence lifetime images were taken for 2 week during differentiation procedure of 3T3-L1 cells into adipocytes. We used 488 nm pulsed laser with 5MHz repetition rate and emission wavelength is 520 nm of Nile Red fluorescent dye. Results clearly show that the lifetime of Nile red in lipid droplets are smaller than those in cell membrane. Our results suggest that fluorescence lifetime imaging can be a very powerful tool to monitor lipid droplet formation in adipocytes from 3T3-L1 cells.

  11. SPIN-ISOSPIN EXCITATIONS IN NUCLEI VIA (HE-3,T) REACTIONS AT 450 MEV

    NARCIS (Netherlands)

    FUJIWARA, M; AKIMUNE, H; DAITO, [No Value; EJIRI, H; FUJITA, Y; GREENFIELD, MB; HARAKEH, MN; HAZAMA, R; INOMATA, T; JANECKE, J; KUDOMI, N; KUME, K; NAKAYAMA, S; SHINMYO, K; TAMII, A; TANAKA, M; TOYOKAWA, H; YOSOI, M

    1994-01-01

    Spin-isospin excitations in nuclei havee been studied by means of the (He-3, t) reactions at 450 MeV. The Gamow-Teller (GT) resonances are found to be strongly excited. A fine structure of the giant Gamow Teller resonances in medium-heavy nuclei is observed with an energy resolution of 130 keV. The

  12. Interactions among groups of microorganisms with rhizosphere Las interacciones entre los grupos de microorganismos en la rizosfera Interações entre grupos de microrganismos com a rizosfera

    OpenAIRE

    Virgínia de Fátima Bezerra Nogueira; Maryzélia Furtado de Farias; Adailson Pereira de Souza; Jussara Silva Dantas

    2010-01-01

    As propriedades físico-químicas da rizosfera têm elevada estabilidade, que, associadas ao fornecimento constante de substratos orgânicos e fatores de crescimento, favorecem intensa atividade metabólica das populações, influenciando diretamente e positivamente o tempo de geração microbiano. O solo é um local de grande número e variedade de interações biológicas, incluindo a competição, a predação, o parasitismo, o comensalismo, o mutualismo e a forésia. As interações b...

  13. Caracterização anatômica e fisiológica da interação porta-enxerto/copa em videiras

    OpenAIRE

    Emiliano Santarosa

    2013-01-01

    A interação entre porta-enxerto e variedade copa pode interferir no equilíbrio vegetativo e produtivo da videira, afetando em alguns casos a morfologia, anatomia e fisiologia das plantas. O objetivo desta pesquisa foi avaliar a interação e influência de porta-enxertos sobre o desenvolvimento vegetativo em mudas de Vitis vinifera, com ênfase em variáveis anatômicas e fisiológicas. Os experimentos foram realizados no período de 2009 a 2012, na casa de vegetação da Faculdade de Agronomia e no La...

  14. Interação entre gene e nutriente e sua associação à obesidade e ao diabetes melito

    OpenAIRE

    Steemburgo,Thais; Azevedo, Mirela Jobim; Martínez, José Alfredo

    2009-01-01

    A genômica nutricional avalia o efeito da variação genética na interação entre dieta e doenças crônicas. O objetivo deste manuscrito foi revisar os principais polimorfismos associados à obesidade, ao diabetes melito e também aos fatores da dieta. As principais interações entre polimorfismos genéticos e dieta foram: para obesidade: interleucina-6 (IL-6) com consumo energético; receptor ativado por proliferador de peroxissoma gama 2 (PPAR-gama2) e fat mass and obesity associated (FTO) com consu...

  15. Estudo das interações medicamentosas em prescrições de pacientes de uma Clínica-Escola de Fisioterapia

    OpenAIRE

    Jean Charles POPETS; Gustavo Batista ANDRADE; Alessandra STROKA ; Sandro ROSTELATO-FERREIRA

    2016-01-01

    Pacientes portadores de doenças crônicas são, em sua maioria, usuários de uma grande quantidade de medicamentos, o que configura polifarmacoterapia. Estes indivíduos apresentam maior possibilidade de sofrerem efeitos nocivos das interações medicamentosas e isso foi tem sido observado em pacientes de uma Clínica-Escola de Fisioterapia no município de Sorocaba. O objetivo desse trabalho foi investigar evidências de interação medicamentosa em prescrições médicas de pacientes da Clíni...

  16. Osteogenic gene expression of murine osteoblastic (MC3T3-E1) cells under cyclic tension

    Science.gov (United States)

    Kao, C. T.; Chen, C. C.; Cheong, U.-I.; Liu, S. L.; Huang, T. H.

    2014-08-01

    Low-level laser therapy (LLLT) can promote cell proliferation. The remodeling ability of the tension side of orthodontic teeth affects post-orthodontic stability. The purpose of the present study was to investigate the osteogenic effects of LLLT on osteoblast-like cells treated with a simulated tension system that provides a mechanical tension regimen. Murine osteoblastic (MC3T3-E1) cells were cultured in a Flexcell strain unit with programmed loads of 12% elongation at a frequency of 0.5 Hz for 24 and 48 h. The cultured cells were treated with a low-level diode laser using powers of 5 J and 10 J. The proliferation of MC3T3-E1 cells was determined using the Alamar Blue assay. The expression of osteogenic genes (type I collagen (Col-1), osteopontin (OPN), osteocalcin (OC), osteoprotegerin (OPG), receptor activator of nuclear factor kappa B ligand (RANKL), bone morphologic protein (BMP-2), and bone morphologic protein (BMP-4)) in MC3T3-E1 cells was analyzed using reverse transcription polymerase chain reaction (RT-PCR). The data were analyzed using one-way analysis of variance. The proliferation rate of tension-cultured MC3T3-E1 cells under 5 J and 10 J LLLT increased compared with that of the control group (p < 0.05). Prominent mineralization of the MC3T3-E1 cells was visible using a von Kossa stain in the 5 J LLLT group. Osteogenic genes (Col-1, OC, OPG and BMP-2) were significantly expressed in the MC3T3-E1 cells treated with 5 J and 10 J LLLT (p < 0.05). LLLT in tension-cultured MC3T3-E1 cells showed synergistic osteogenic effects, including increases in cell proliferation and Col-1, OPN, OC, OPG and BMP-2 gene expression. LLLT might be beneficial for bone remodeling on the tension side of orthodontics.

  17. Berberine activates GLUT1-mediated glucose uptake in 3T3-L1 adipocytes.

    Science.gov (United States)

    Kim, So Hui; Shin, Eun-Jung; Kim, Eun-Do; Bayaraa, Tsenguun; Frost, Susan Cooke; Hyun, Chang-Kee

    2007-11-01

    It has recently been known that berberine, an alkaloid of medicinal plants, has anti-hyperglycemic effects. To explore the mechanism underlying this effect, we used 3T3-L1 adipocytes for analyzing the signaling pathways that contribute to glucose transport. Treatment of berberine to 3T3-L1 adipocytes for 6 h enhanced basal glucose uptake both in normal and in insulin-resistant state, but the insulin-stimulated glucose uptake was not augmented significantly. Inhibition of phosphatidylinositol 3-kinase (PI 3-K) by wortmannin did not affect the berberine effect on basal glucose uptake. Berberine did not augment tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate (IRS)-1. Further, berberine had no effect on the activity of the insulin-sensitive downstream kinase, atypical protein kinase C (PKCzeta/lambda). However, interestingly, extracellular signal-regulated kinases (ERKs), which have been known to be responsible for the expression of glucose transporter (GLUT)1, were significantly activated in berberine-treated 3T3-L1 cells. As expected, the level of GLUT1 protein was increased both in normal and insulin-resistant cells in response to berberine. But berberine affected the expression of GLUT4 neither in normal nor in insulin-resistant cells. In addition, berberine treatment increased AMP-activated protein kinase (AMPK) activity in 3T3-L1 cells, which has been reported to be associated with GLUT1-mediated glucose uptake. Together, we concluded that berberine increases glucose transport activity of 3T3-L1 adipocytes by enhancing GLUT1 expression and also stimulates the GLUT1-mediated glucose uptake by activating GLUT1, a result of AMPK stimulation.

  18. The effect of MAGE-A1 gene on NIH3T3 cells

    Institute of Scientific and Technical Information of China (English)

    Jingjun Sun; Jin Zhu; Zhenning Qiu; Yuhua Li; Guipeng Ding; Yi Zhu; Zhenqing Feng; Xiaohong Guan

    2005-01-01

    Objective: Melanoma antigen genes(MAGE) genes have been found in many kinds of tumor tissue, but not in normal tissue except testis and placentas. The Ags encoded by MAGE genes therefore are strictly tumor-specific. The most current researches associated with these genes focus on the tumor vaccination using these Ags. Few reports are concerning these genes' functions. In this study, we investigated the role of MAGE-A1 gene on NIH3T3 cells after transferring with it. Methods: Clone the MAGE-A1 into the plasmids pEGFP-C3 and pcDNA3.1, then transfer the reconstructed plasmids and primary plasmids into the NIH3T3 cells using a new transfer reagent FuGENE 6. Selecting the positively transferred cells by G418. Identified by RT-PCR, Western blot, Immunocytochemistry,Laser Scanning Confocal Microscope and Fluoroscope. The cells mobile ability was measured with Millicell-PCF. The cell cycle and apoptosis were measured with Flow Cytometry. Results: The apoptosis rate of NIH3T3 cells that transferred with control plasmid pcDNA3.1was 13.4% and the raitos that stay in S phase and G2-M phase were 5.68% and 1.04% respectively. The apoptosis rate of NIH3T3 cells that transferred with pcDNA3.1-A1 was 0.90% and the ratios that stayed in S phase and G2-M phase were 19.31% and 13.47% respectively. The apoptosis rate of the cells that transferred with control plasmid pEGFP-C3 was 1.87 %, a little higher than 1.47 % of those transferred with pEGFP-C3-A1. Conclusion: The MAGE-A1 gene may enhance the cell cycle, inhibit the apoptosis and raise the mobile ability of NIH3T3 cells.

  19. Age dependency of myocardial triglyceride content. A 3T high-field {sup 1}H-MR spectroscopy study

    Energy Technology Data Exchange (ETDEWEB)

    Petritsch, B.; Gassenmaier, T.; Kunz, A.S.; Donhauser, J.; Bley, T.A.; Horn, M. [University Hospital of Wuerzburg (Germany). Inst. of Diagnostic and Interventional Radiology; Goltz, J.P. [University Hospital of Schleswig-Holstein, Campus Luebeck (Germany). Clinic for Radiology and Nuclear Medicine

    2015-11-15

    The role of myocardial triglyceride (mTG) content in the aging human heart is not entirely understood. The aim of this study was to measure concentrations of mTG content from healthy volunteers and to determine the association between age, mTG content and systolic heart function. Furthermore, the technical stability of the {sup 1}H-magnetic resonance spectroscopy ({sup 1}H-MRS) and the reliability of peak evaluation at 3 T were evaluated. The total study population of 47 healthy volunteers was divided into 4 age classes, according to the age of the subjects (1{sup st} cohort 20-29 years (yrs.), n=20; 2{sup nd} cohort 30-39 yrs., n=10; 3{sup rd} cohort 40-49 yrs., n=9; 4{sup th} cohort 50-60 yrs., n=8). Cardiac MRI and double triggered {sup 1}H-MRS of the myocardium were consecutively performed using a 3 T scanner. Each participant underwent spectroscopic measurements twice in the same investigation. mTG content increases with age. The correlation of age and mTG is minimal (r=0.48; p<0.001). The following age-averaged mTG content values expressed as % of mTG signal compared to the water signal were determined for each cohort: 1{sup st} cohort 0.25 % (± 0.17); 2{sup nd} cohort 0.48 % (± 0.30); 3{sup rd} cohort 0.48 % (± 0.18); 4{sup th} cohort 0.77 % (± 0.70). There was no significant correlation (r=0.04; p=n.s.) between LV mass and mTG content in healthy volunteers. Within our cohorts, no effects of age or mTG content on systolic heart function were seen (r=-0.01; p=n.s.). The intraclass correlation coefficient of spectroscopic measurements was high (r=0.965; p<0.001). Myocardial TG content increases with age. The normal age-dependent concentration ranges of myocardial lipid metabolites reported in this study may be helpful for the correction of acquired {sup 1}H-MRS data in patients when evaluating metabolic and cardiovascular diseases in future magnetic resonance spectroscopy studies.

  20. Fusion in diffusion MRI for improved fibre orientation estimation: An application to the 3T and 7T data of the Human Connectome Project.

    Science.gov (United States)

    Sotiropoulos, Stamatios N; Hernández-Fernández, Moisés; Vu, An T; Andersson, Jesper L; Moeller, Steen; Yacoub, Essa; Lenglet, Christophe; Ugurbil, Kamil; Behrens, Timothy E J; Jbabdi, Saad

    2016-07-01

    Determining the acquisition parameters in diffusion magnetic resonance imaging (dMRI) is governed by a series of trade-offs. Images of lower resolution have less spatial specificity but higher signal to noise ratio (SNR). At the same time higher angular contrast, important for resolving complex fibre patterns, also yields lower SNR. Considering these trade-offs, the Human Connectome Project (HCP) acquires high quality dMRI data for the same subjects at different field strengths (3T and 7T), which are publically released. Due to differences in the signal behavior and in the underlying scanner hardware, the HCP 3T and 7T data have complementary features in k- and q-space. The 3T dMRI has higher angular contrast and resolution, while the 7T dMRI has higher spatial resolution. Given the availability of these datasets, we explore the idea of fusing them together with the aim of combining their benefits. We extend a previously proposed data-fusion framework and apply it to integrate both datasets from the same subject into a single joint analysis. We use a generative model for performing parametric spherical deconvolution and estimate fibre orientations by simultaneously using data acquired under different protocols. We illustrate unique features from each dataset and how they are retained after fusion. We further show that this allows us to complement benefits and improve brain connectivity analysis compared to analyzing each of the datasets individually.

  1. Evaluation of acute renal colic: a comparison of non-contrast CT versus 3-T non-contrast HASTE MR urography.

    Science.gov (United States)

    Semins, M J; Feng, Z; Trock, B; Bohlman, M; Hosek, W; Matlaga, B R

    2013-02-01

    With the introduction of a 3-T scanner, magnetic resonance urography (MRU) may be an alternative imaging modality for evaluation of acute renal colic. We performed a prospective study to compare the performance of computed tomography (CT) with half-Fourier single shot turbo spin-echo (HASTE) MRU in the evaluation of patients with suspected renal colic. Patients presenting to the emergency department with acute renal colic were eligible for inclusion. Following a standard CT stone evaluation, patients underwent a non-contrast HASTE MRU study with a 3-T scanner. The presence of perinephric fluid, hydronephrosis, ureteral obstruction, and calculus was assessed. A total of 22 patients completed the study. Twenty (91 %) were diagnosed with an upper tract stone by radiographic findings. MRU detected a discrete stone in 50 % of the patients with stones detected by CT. Perinephric fluid was noted in 12 MRUs, compared to 7 CTs. Using CT as the reference standard, the combination of stone or perinephric fluid and ureteral dilation gave MRU a sensitivity of 84 %, specificity of 100 %, and accuracy of 86 % (95 % CI 0.72-1.0). HASTE MRU with a 3-T MR scanner can reliably detect the presence of upper urinary tract obstruction. Although CT imaging remains the superior modality with which to detect calculi, MRU detects a greater number of secondary signs of upper tract obstruction. For situations in which the use of ionizing radiation is undesirable, MRU is a reasonable imaging alternative.

  2. A new semi-quantitative approach for analysing 3T diffusion tensor imaging of optic fibres and its clinical evaluation in glaucoma.

    Science.gov (United States)

    Engelhorn, Tobias; Haider, Sultan; Michelson, Georg; Doerfler, Arnd

    2010-10-01

    Diffusion tensor imaging can depict rarefaction of the optical fibers. Manual segmentation is time consuming. The purposes of the study were (1) to present a new semiquantitative segmentation approach for analyzing 3-T diffusion tensor imaging of optical fibers and (2) to clinically test the new approach by comparing optic fiber rarefaction in patients with glaucoma to that in age-matched, healthy controls. To perform semiautomated and quantitative segmentation of the optical radiation, a Mathcad-based software program was developed. The results were compared to the manual evaluation of the images performed by two experienced neuroradiologists. The eyes of 42 subjects (22 patients with glaucoma and 20 controls) aged 37 to 86 years were assessed in full ophthalmologic examinations. Magnetic resonance imaging was performed using a 3-T high-field scanner. The evaluation using the new approach matched 94% with manually acquired rarefaction of the optical radiation; Cronbach's α was >0.81 for calculation of the manually and semiautomatically derived volumes. The new approach seems to be robust and is clearly faster compared to the more tedious manual segmentation. Using diffusion tensor imaging at 3 T, it could be shown that there was increasing atrophy of the optical radiation (fourth neuron) with increasing age in patients with glaucoma. Compared to age-matched, healthy patients, more pronounced atrophy of the fourth neuron was found in patients with glaucoma. Copyright © 2010 AUR. Published by Elsevier Inc. All rights reserved.

  3. Superficial amygdala and hippocampal activity during affective music listening observed at 3 T but not 1.5 T fMRI.

    Science.gov (United States)

    Skouras, Stavros; Gray, Marcus; Critchley, Hugo; Koelsch, Stefan

    2014-11-01

    The purpose of this study was to compare 3 T and 1.5 T fMRI results during emotional music listening. Stimuli comprised of psychoacoustically balanced instrumental musical pieces, with three different affective expressions (fear, neutral, joy). Participants (N=32) were split into two groups, one subjected to fMRI scanning using 3 T and another group scanned using 1.5 T. Whole brain t-tests (corrected for multiple comparisons) compared joy and fear in each of the two groups. The 3 T group showed significant activity differences between joy and fear localized in bilateral superficial amygdala, bilateral hippocampus and bilateral auditory cortex. The 1.5 T group showed significant activity differences between joy and fear localized in bilateral auditory cortex and cuneus. This is the first study to compare results obtained under different field strengths with regard to affective processes elicited by means of auditory/musical stimulation. The findings raise concern over false negatives in the superficial amygdala and hippocampus in affective studies conducted under 1.5 T and caution that imaging improvements due to increasing magnetic field strength can be influenced by region-specific characteristics.

  4. Cell Volume Regulation and Signaling in 3T3-L1 Pre-adipocytes and Adipocytes

    DEFF Research Database (Denmark)

    Eduardsen, Kathrine; Larsen, Susanne; Novak, Ivana

    2011-01-01

    Caveolae have been implicated in sensing of cell volume perturbations, yet evidence is still limited and findings contradictory. Here, we investigated the possible role of caveolae in cell volume regulation and volume sensitive signaling in an adipocyte system with high (3T3-L1 adipocytes......) and osmotic cell shrinkage (RVI), accompanied by increased swelling-activated taurine efflux. However, caveolin-1 distribution was not detectably altered after osmotic swelling or shrinkage, and caveolae integrity, as studied by cholesterol depletion or expression of dominant negative Cav-1, was not required...... no detectable effect on InsR, yet increased ERK1/2 phosphorylation. In conclusion, differentiated 3T3-L1 adipocytes exhibit greatly accelerated RVD and RVI responses and increased swelling-activated taurine efflux compared to pre-adipocytes. Furthermore, in pre-adipocytes, Cav-1/caveolae integrity...

  5. Pleiotrophin Transforms NIH 3T3 Cells and Induces Tumors in Nude Mice

    Science.gov (United States)

    Chauhan, Anil K.; Li, Yue-Sheng; Deuel, Thomas F.

    1993-01-01

    The pleiotrophin (PTN) gene (Ptn) encodes an 18-kDa protein that is highly conserved among mammalian species and that functions as a weak mitogen and promotes neurite-outgrowth activity in vitro. To further investigate the role PTN plays in regulating cell growth, we overexpressed the bovine PTN cDNA and now show that PTN phenotypically transforms NIH 3T3 cells, as evidence by increased cell number at confluence, focus formation, anchorage-independent growth, and tumor formation in the nude muse. The results demonstrate that the Ptn gene has the potential to regulate NIH 3T3 cell growth and suggest that PTN may influence abnormal cell growth in vivo.

  6. High-quality genome sequence and description of Bacillus ndiopicus strain FF3T sp. nov.

    Directory of Open Access Journals (Sweden)

    C.I. Lo

    2015-11-01

    Full Text Available Strain FF3T was isolated from the skin-flora of a 39-year-old healthy Senegalese man. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry did not allow any identification. This strain exhibited a 16S rRNA sequence similarity of 96.8% with Bacillus massiliensis, the phylogenetically closest species with standing nomenclature. Using a polyphasic study made of phenotypic and genomic analyses, strain FF3T was Gram-positive, aeroanaerobic and rod shaped and exhibited a genome of 4 068 720 bp with a G+C content of 37.03% that coded 3982 protein-coding and 67 RNA genes (including four rRNA operons. On the basis of these data, we propose the creation of Bacillus ndiopicus sp. nov.

  7. Influência do LPS e do zinco na interação mãe e filhote

    OpenAIRE

    Amanda Florentina do Nascimento

    2016-01-01

    O comportamento materno consiste em um conjunto de mudanças comportamentais e fisiológicas, exercidas pelos indivíduos adultos em torno dos indivíduos reprodutivamente imaturos, garantindo sua sobrevivência e a propagação de sua espécie. A interação mãe e filhote é tida tipicamente como simbiótica. Os filhotes quando separados da mãe sinalizam para serem recolhidos através de dicas olfativas, visuais e da vocalização que representa uma forma de comunicação filhote e mãe. O modelo de febre clá...

  8. Razão, regulação e ritmos nas interações discursivas

    Directory of Open Access Journals (Sweden)

    Jairo Ferreira

    2001-02-01

    Full Text Available A compreensão dos processos cognitivos em interações discursivas nos marcos do estruturalismo genético de Jean Piaget permite uma síntese entre a compreensão dos processos lógicos e operatórios sugeridos pelo racionalismo, das regulações sociais através da negociação e contratos entre os interlocutores conforme a análise do discurso (Charaudeau e dos ritmos (inclusive os rituais observados pela antropologia.  O artigo discute esta perspectiva genética e depois, por inferência, sugere ligações teóricas e de método para análise sócio-cognitiva do discurso.

  9. A epistemologia genética e a interação social de crianças

    OpenAIRE

    Moro Maria Lucia Faria

    2000-01-01

    O artigo apresenta um estudo de caso sobre as relações da interação social de crianças com suas construções cognitivas individuais de aprendizagem da adição/subtração, segundo proposições da epistemologia genética. Os resultados vêm da análise qualitativa microgenética da seqüência completa, gravada em vídeo, dos eventos referentes às inter-relações: a) das estratégias cognitivas expressas por três meninos que, juntos, resolveram tarefas relativas ao conteúdo aritmético citado; b) daquelas es...

  10. Interações sociais de crianças pré-escolares com Síndrome de Down durante atividades extracurriculares

    Directory of Open Access Journals (Sweden)

    Renata Valdívia Lucisano

    2013-02-01

    Full Text Available O objetivo desta pesquisa foi identificar o processo de interação social de crianças com Síndrome de Down (SD durante atividades extracurriculares na rede regular de educação infantil do município de Ribeirão Preto. Participaram da pesquisa seis crianças na faixa etária de 3 a 6 anos. Foram realizadas duas filmagens de cada criança em situações de interação social durante atividades extracurriculares, e analisadas através de quinze comportamentos, distribuídos em duas categorias de habilidades sociais: interpessoais e de autoexpressão. Os resultados demonstraram que, quanto às habilidades interpessoais, houve maior ocorrência do comportamento "ocorre interação com outra criança". Já na categoria habilidades de autoexpressão, apenas os comportamentos "sorri" e "imita outras crianças" apresentam ocorrência significativa. Os comportamentos mais frequentes encontrados neste estudo permitem compreender que o ambiente escolar torna-se um facilitador na interação da criança com SD com outras crianças de desenvolvimento típico, possibilitando o desenvolvimento das habilidades sociais esperadas.

  11. Interação Social e Mediação em Ambientes Digitais de Aprendizagem com Sujeitos com Autismo

    Directory of Open Access Journals (Sweden)

    Liliana M Passerino

    2009-07-01

    Full Text Available A partir de uma concepção sócio-histórica da interação social e
    considerando as Tecnologias de Comunicação e Informação  (TICs disponíveis atualmente para a construção de Ambientes Digitais de Aprendizagem (ADA procurou-se sanar a inexistência de dados empíricos relativos ao fenômeno de interação social de pessoas autistas mediado pela tecnologia. A presente pesquisa propõe uma visão diferenciada sobre os processos de interação e mediação que se estabelecem em ambientes digitais. Estruturada como um estudo multicasos com observação de grupos de autistas em atividades de
    interação mediadas por ADAs ao longo de 3 anos  identificaram-se categorias relevantes emergentes dessa mediação tecnológica com contribuições importantes para as areas envolvidas (Psicologia, Educação e Computação na construção de um ambiente digital de aprendizagem especialmente projetado levando em conta os resultados obtidos.

  12. Interações sociais de crianças pré-escolares com Síndrome de Down durante atividades extracurriculares

    Directory of Open Access Journals (Sweden)

    Renata Valdívia Lucisano

    Full Text Available O objetivo desta pesquisa foi identificar o processo de interação social de crianças com Síndrome de Down (SD durante atividades extracurriculares na rede regular de educação infantil do município de Ribeirão Preto. Participaram da pesquisa seis crianças na faixa etária de 3 a 6 anos. Foram realizadas duas filmagens de cada criança em situações de interação social durante atividades extracurriculares, e analisadas através de quinze comportamentos, distribuídos em duas categorias de habilidades sociais: interpessoais e de autoexpressão. Os resultados demonstraram que, quanto às habilidades interpessoais, houve maior ocorrência do comportamento "ocorre interação com outra criança". Já na categoria habilidades de autoexpressão, apenas os comportamentos "sorri" e "imita outras crianças" apresentam ocorrência significativa. Os comportamentos mais frequentes encontrados neste estudo permitem compreender que o ambiente escolar torna-se um facilitador na interação da criança com SD com outras crianças de desenvolvimento típico, possibilitando o desenvolvimento das habilidades sociais esperadas.

  13. Interação entre gene e nutriente e sua associação à obesidade e ao diabetes melito

    National Research Council Canada - National Science Library

    Steemburgo, Thais; Azevedo, Mirela J.de; Martínez, José Alfredo

    2009-01-01

    ... e também aos fatores da dieta. As principais interações entre polimorfismos genéticos e dieta foram: para obesidade: interleucina-6 (IL-6) com consumo energético; receptor ativado por proliferador de peroxissoma gama 2...

  14. The intracellular mechanism of alpha-fetoprotein promoting the proliferation of NIH 3T3 cells

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    AIM The existence and properties of alpha-fetoprotein (AFP) receptor on the surface of NIH 3T3 cells and the effects of AFP on cellular signal transduction pathway were investigated. METHODS The effect of AFP on the proliferation of NIH 3T3 cells was measured by incorporation of 3H-TdR. Receptor-binding assay of 125I-AFP was performed to detect the properties of AFP receptor in NIH 3T3 cells. The influences of AFP on the [cAMP]i and the activities of protein kinase A (PKA) were determined. Western blot was used to detect the change of K-ras P21 protein expression. RESULTS The proliferation of NIH 3T3 cells treated with 0-80 mg/L of AFP was significantly enhanced. The Scatchard analysis indicated that there were two classes of binding sites with KD of 2.722×10-9M (Bmax=12810 sites per cell) and 8.931× 10-SM (Bmax=l19700 sites per cell) respectively. In the presence of AFP (20 mg/L), the content of cAMP and activities of PKA were significantly elevated . The level of K-ras P21 protein was upregulated by AFP at the concentration of 20 mg/L. The monoclonal antibody against AFP could reverse the effects of AFP on the cAMP content, PKA activity and the expression of K-ras p21 gene. CONCLUSION The effect of AFP on the cell proliferation was achieved by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 gene.

  15. Ultrasound associated uptake of chitosan nanoparticles in MC3T3-E1 cells

    Science.gov (United States)

    Wu, Junyi

    Chitosan is a natural linear polysaccharide that has been well known for its applications in drug delivery system due to its unique physicochemical and biological properties. However, challenges still remain for it to become a fully realized therapeutic agent. In this study, we investigated the uptake of chitosan nanoparticles (CNP) under the ultrasound stimulation, using a model cell culture system (MC3T3-E1 mouse pre-osteoblasts). The CNP were fabricated by an ionic gelation method and were lyophilized prior to characterization and delivery to cells. Particle size and zeta potential were measured using Dynamic Light Scattering (DLS); the efficiency of chitosan complexation was measured using the ninhydrin assay. Cytotoxicity was examined by neutral red assay within 48 hours after delivery. The effect of ultrasound (US) on the efficiency of nanoparticle delivery to the MC3T3-E1 cells was examined at 1MHz and at either 1 or 2 W/cm2. Fluorescein isothiocyanate (FITC)-conjugated-CNP were used to visualize the internalized particles within the cytosol. The uptake of FITC-CNP exhibits a dose and time dependent effect, a strong FITC fluorescence was detected at the concentration of 500microg/mL under fluorescence microscope. Ultrasound assisted uptake of FITC-CNP performed a significant positive effect at 2W/cm2 with 60s of ultrasound exposure time. CNP displayed a slightly decrease in cell viability from 25microg/mL to 100microg/mL, while higher concentration of CNP facilitates the proliferation of MC3T3-E1 cells. Less than 10% of reduction in cell viability was observed for US at 1W/cm2 and 2W/cm2 with 30s and 60s of exposure time, which suggest a mild effect of US to MC3T3-E1 cell line.

  16. Quantifying coronary sinus flow and global LV perfusion at 3T

    Directory of Open Access Journals (Sweden)

    Bloch Karin

    2009-06-01

    Full Text Available Abstract Background Despite the large availability of 3T MR scanners and the potential of high field imaging, this technical platform has yet to prove its usefulness in the cardiac MR setting, where 1.5T remains the established standard. Global perfusion of the left ventricle, as well as the coronary flow reserve (CFR, can provide relevant diagnostic information, and MR measurements of these parameters may benefit from increased field strength. Quantitative flow measurements in the coronary sinus (CS provide one method to investigate these parameters. However, the ability of newly developed faster MR sequences to measure coronary flow during a breath-hold at 3T has not been evaluated. Methods The aim of this work was to measure CS flow using segmented phase contrast MR (PC MR on a clinical 3T MR scanner. Parallel imaging was employed to reduce the total acquisition time. Global LV perfusion was calculated by dividing CS flow with left ventricular (LV mass. The repeatability of the method was investigated by measuring the flow three times in each of the twelve volunteers. Phantom experiments were performed to investigate potential error sources. Results The average CS flow was determined to 88 ± 33 ml/min and the deduced LV perfusion was 0.60 ± 0.22 ml/min·g, in agreement with published values. The repeatability (1-error of the three repeated measurements in each subject was on average 84%. Conclusion This work demonstrates that the combination of high field strength (3T, parallel imaging and segmented gradient echo sequences allow for quantification of the CS flow and global perfusion within a breath-hold.

  17. The Ga-71(He-3, t) reaction and the low-energy neutrino response

    NARCIS (Netherlands)

    Frekers, D.; Ejiri, H.; Akimune, H.; Adachi, T.; Bilgier, B.; Brown, B. A.; Cleveland, B. T.; Fujita, H.; Fujita, Y.; Fujiwara, M.; Ganioglu, E.; Gavrin, V. N.; Grewe, E. -W.; Guess, C. J.; Harakeh, M. N.; Hatanaka, K.; Hodak, R.; Holl, M.; Iwamoto, C.; Khai, N. T.; Kozer, H. C.; Lennarz, A.; Okamoto, A.; Okamura, H.; Povinec, P. P.; Puppe, P.; Simkovic, F.; Susoy, G.; Suzuki, T.; Tamii, A.; Thies, J. H.; Van de Walle, J.; Zegers, R. G. T.

    2011-01-01

    A Ga-71(He-3,t)Ge-71 charge-exchange experiment was performed to extract with high precision the Gamow-Teller (GT) transition strengths to the three lowest-lying states in Ge-71, i.e., the ground state (1/2(-)), the 175 keV (5/2(-)) and the 500 key (3/2(-)) excited states. These are the relevant sta

  18. Visitando a interação na prosa literária Visiting the interaction in the literary prose

    Directory of Open Access Journals (Sweden)

    Dóris de Arruda C. da Cunha

    2008-01-01

    Full Text Available Este artigo tem o objetivo analisar a interação por meio dos discursos no romance, partindo do princípio que não são formas sintáticas de transmissão das falas e dos pensamentos das personagens, mas representações da interação entre sujeitos e suas posições axiológicas. Analisa a relação entre Lingüística e Literatura, partilhando a visão de Maingueneau segundo a qual não se pode continuar a pensar dicotomicamente essa relação, uma vez que as duas ciências da linguagem trazem novas descobertas para o texto literário. Tomando por base a teoria bakhtiniana, faz um estudo dos discursos num fragmento de Capitães da Areia, que revela o embate de visões de mundo, a das elites e a dos excluídos, representados artisticamente nesse romance.The aim of this paper is to analyse the interaction in the novel, starting from the principle that discourses can not be structured as reported speeches, for they are representations of the interaction between subjects and their axiological positions. This paper also analyses the relationship between Linguistics and Literature, sharing Maingueneau's view that one can not continue to think this relationship dichotomically, for both sciences of language bring new discoveries for the literary text. Taking Bakhtinian discourse theory as our base, this article studies the discourses in a fragment of Capitães de Areia, which reveals the clash of views of the world: that of the elite and that of the excluded, represented artistically in the novel.

  19. Stevioside from Stevia rebaudiana Bertoni Increases Insulin Sensitivity in 3T3-L1 Adipocytes

    Directory of Open Access Journals (Sweden)

    Nabilatul Hani Mohd-Radzman

    2013-01-01

    Full Text Available Stevioside from Stevia rebaudiana has been reported to exert antihyperglycemic effects in both rat and human subjects. There have been few studies on these effects in vitro. In this paper, radioactive glucose uptake assay was implemented in order to assess improvements in insulin sensitivity in 3T3-L1 cells by elevation of glucose uptake following treatment with stevioside. Oil Red-O staining and MTT assay were utilized to confirm adipocyte differentiation and cell viability, respectively. Findings from this research showed a significant increase in absorbance values in mature adipocytes following Oil Red-O staining, confirming the differentiation process. Stevioside was noncytotoxic to 3T3-L1 cells as cell viability was reduced by a maximum of 17%, making it impossible to determine its IC50. Stevioside increased glucose uptake activities by 2.1 times (p<0.001 in normal conditions and up to 4.4 times (p<0.001 in insulin-resistant states. At times, this increase was higher than that seen in positive control group treated with rosiglitazone maleate, an antidiabetic agent. Expressions of pY20 and p-IRS1 which were measured via Western blot were improved by stevioside treatment. In conclusion, stevioside has direct effects on 3T3-L1 insulin sensitivity via increase in glucose uptake and enhanced expression of proteins involved in insulin-signalling pathway.

  20. Rubi Fructus (Rubus coreanus) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells.

    Science.gov (United States)

    Jeong, Mi-Young; Kim, Hye-Lin; Park, Jinbong; An, Hyo-Jin; Kim, Sung-Hoon; Kim, Su-Jin; So, Hong-Seob; Park, Raekil; Um, Jae-Young; Hong, Seung-Heon

    2013-01-01

    Rubi Fructus (RF) is known to exert several pharmacological effects including antitumor, antioxidant, and anti-inflammatory activities. However, its antiobesity effect has not been reported yet. This study was focused on the antidifferentiation effect of RF extract on 3T3-L1 preadipocytes. When 3T3-L1 preadipocytes were differentiating into adipocytes, 10-100  μ g/mL of RF was added. Next, the lipid contents were quantified by Oil Red O staining. RF significantly reduced lipid accumulation and downregulated the expression of peroxisome proliferator-activated receptor γ (PPAR γ ), CCAAT0-enhancer-binding proteins α (C/EBP α ), adipocyte fatty acid-binding protein 2 (aP2), resistin, and adiponectin in ways that were concentration dependent. Moreover, RF markedly upregulated liver kinase B1 and AMP-activated protein kinase (AMPK). Interestingly, pretreatment with AMPK α siRNA and RF downregulated the expression of PPAR γ and C/EBP α protein as well as the adipocyte differentiation. Our study shows that RF is capable of inhibiting the differentiation of 3T3-L1 adipocytes through the modulation of PPAR γ , C/EBP α , and AMPK, suggesting that it has a potential for therapeutic application in the treatment or prevention of obesity.

  1. Effect of Mangiferin and Mahanimbine on Glucose Utilization in 3T3-L1 cells

    Science.gov (United States)

    Kumar, B Dinesh; Krishnakumar, K; Jaganathan, Saravana Kumar; Mandal, Mahitosh

    2013-01-01

    Background: Stem barks of Mangifera indica contain a rich content of mangiferin (xanthone glucoside), whereas Murraya koenigii leaves contain rich sources of mahanimbine (carbazole alkaloid) and used traditionally for the treatment of diabetes. Objective: To investigate the effects of mangiferin (xanthone glucoside) and mahanimbine (carbazole alkaloid) on glucose utilization in 3T3-L1 cells. Materials and Methods: Mangiferin was isolated from stem barks of Mangifera indica and mahanimbine was isolated from Murraya koenigii leaves. These isolated compounds were subjected to MTT assay and glucose utilization test with 3T3-L1 cells. Results: Treatment of the 3T3-L1 cells with mangiferin and mahanimbine increased the glucose utilization in a dose-dependent manner. At a concentration of 1 mM, mangniferin showed 2-fold increase in glucose utilization compared with untreated control. In case of mahanimbine, the observed effect at 1 mM was almost equivalent to positive control (insulin at 1 μM). Moreover, MTT assay showed that both of these compounds were less toxic at a concentration of 1 mM (nearly 75% cells are viable). Conclusion: The present results indicated that these natural products (mangiferin and mahanimbine) exhibited potential ethnomedical uses in management of diabetes. PMID:23661997

  2. WEHI-3 cells inhibit adipocyte differentiation in 3T3-L1 cells

    Energy Technology Data Exchange (ETDEWEB)

    Lai, Jing [The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong (China); Liu, Gexiu [Institute of Hematology, School of Medicine, Jinan University, Guangzhou, Guangdong (China); Yan, Guoyao [The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong (China); He, Dongmei [Institute of Hematology, School of Medicine, Jinan University, Guangzhou, Guangdong (China); Zhou, Ying [The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong (China); Chen, Shengting, E-mail: shengtingchen@sina.cn [The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong (China)

    2015-06-26

    By investigating the anti-adipogenic effects of WEHI-3 cells – a murine acute myelomonocytic leukemia cell line – we sought to improve the efficiency of hematopoietic stem cell transplantation (HSCT). Analysis of Oil Red O staining and the expression of adipogenic genes, including PPARγ, C/EBPα, FAS and LPL, indicated that WEHI-3 cells significantly inhibited 3T3-L1 mouse preadipocyte cells from differentiating into adipocytes. In vivo, fat vacuoles in mice injected with WEHI-3 cells were also remarkably reduced in the murine bone marrow pimelosis model. Moreover, the key gene in the Rho signaling pathway, ROCKII, and the key gene in the Wnt signaling pathway, β-catenin, were both upregulated compared with the control group. siRNA-mediated knockdown of ROCKII and β-catenin reversed these WEHI-3-mediated anti-adipogenic effects. Taken together, these data suggest that WEHI-3 cells exert anti-adipogenic effects and that both ROCKII and β-catenin are involved in this process. - Highlights: • WEHI-3, an acute myelomonocytic leukemia cell line, inhibited 3T3-L1 preadipocyte from differentiating into adipocyte. • WEHI-3 cells can arrest 3T3-L1 cells in G0/G1 phase by secreting soluble factors and thus inhibit their proliferation. • WEHI-3 cells reduced bone marrow pimelosis in the murine model. • Both ROCKII and β-catenin were involved in the WEHI-3-mediated anti-adipogenic effects.

  3. Effects of 6-Hydroxyflavone on Osteoblast Differentiation in MC3T3-E1 Cells

    Directory of Open Access Journals (Sweden)

    Chien-Hung Lai

    2014-01-01

    Full Text Available Osteoblast differentiation plays an essential role in bone integrity. Isoflavones and some flavonoids are reported to have osteogenic activity and potentially possess the ability to treat osteoporosis. However, limited information concerning the osteogenic characteristics of hydroxyflavones is available. This study investigates the effects of various hydroxyflavones on osteoblast differentiation in MC3T3-E1 cells. The results showed that 6-hydroxyflavone (6-OH-F and 7-hydroxyflavone (7-OH-F stimulated ALP activity. However, baicalein and luteolin inhibited ALP activity and flavone showed no effect. Up to 50 μM of each compound was used for cytotoxic effects study; flavone, 6-OH-F, and 7-OH-F had no cytotoxicity on MC3T3-E1 cells. Moreover, 6-OH-F activated AKT and serine/threonine kinases (also known as protein kinase B or PKB, extracellular signal-regulated kinases (ERK 1/2, and the c-Jun N-terminal kinase (JNK signaling pathways. On the other hand, 7-OH-F promoted osteoblast differentiation mainly by activating ERK 1/ 2 signaling pathways. Finally, after 5 weeks of 6-OH-F induction, MC3T3-E1 cells showed a significant increase in the calcein staining intensity relative to merely visible mineralization observed in cells cultured in the osteogenic medium only. These results suggested that 6-OH-F could activate AKT, ERK 1/2, and JNK signaling pathways to effectively promote osteoblastic differentiation.

  4. Cyclopia maculata (honeybush tea) stimulates lipolysis in 3T3-L1 adipocytes.

    Science.gov (United States)

    Pheiffer, Carmen; Dudhia, Zulfaqar; Louw, Johan; Muller, Christo; Joubert, Elizabeth

    2013-10-15

    We have previously, for the first time, demonstrated that hot water extracts of Cyclopia maculata and Cyclopia subternata, endemic South African plants that are consumed as herbal teas, inhibit adipogenesis in 3T3-L1 adipocytes. The aim of this study was to extend the anti-obesity investigations of these plants by quantifying lipolysis in mature 3T3-L1 adipocytes. Glycerol concentration in culture supernatants was used as a marker of adipocyte lipolysis. Isoproterenol, a β-adrenergic agonist and a known lipolytic agent, was used as a positive control in our assays. Lipolysis was stimulated by all extracts, although statistical significance was noted for fermented (oxidised) C. maculata only. A concentration of 80μg/ml of C. maculata extract induced maximal lipolysis (1.8-fold, plipolysis was accompanied by an increase in the expression of hormone sensitive lipase (1.6-fold, plipolysis in mature 3T3-L1 adipocytes, providing further support for the anti-obesity effects of Cyclopia spp. Copyright © 2013 Elsevier GmbH. All rights reserved.

  5. Endoplasmic reticulum stress suppresses lipin-1 expression in 3T3-L1 adipocytes

    Energy Technology Data Exchange (ETDEWEB)

    Takahashi, Nobuhiko, E-mail: ntkhs@hoku-iryo-u.ac.jp [Department of Internal Medicine, School of Dentistry, Health Sciences University of Hokkaido, 1757, Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan); Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1, Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510 (Japan); Yoshizaki, Takayuki [Innovation Center, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890-0065 (Japan); Hiranaka, Natsumi; Suzuki, Takeshi [Department of Internal Medicine, School of Dentistry, Health Sciences University of Hokkaido, 1757, Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan); Yui, Tomoo; Akanuma, Masayoshi [Department of Fixed Prosthodontics and Oral Implantology, School of Dentistry, Health Sciences University of Hokkaido, 1757, Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan); Kanazawa, Kaoru [Department of Dental Anesthesiology, School of Dentistry, Health Sciences University of Hokkaido, 1757, Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan); Yoshida, Mika; Naito, Sumiyoshi [Department of Clinical Laboratory, Health Sciences University of Hokkaido, 1757, Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan); Fujiya, Mikihiro; Kohgo, Yutaka [Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1, Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510 (Japan); Ieko, Masahiro [Department of Internal Medicine, School of Dentistry, Health Sciences University of Hokkaido, 1757, Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan)

    2013-02-01

    Highlights: ► Lipin-1 involves lipid metabolism, adipocyte differentiation, and inflammation. ► Adipose lipin-1 expression is reduced in obesity. ► ER stress suppresses lipin-1 expression in 3T3-L1 adipocytes. ► Activation of PPAR-γ recovers ER stress-induced lipin-1 reduction. -- Abstract: Lipin-1 plays crucial roles in the regulation of lipid metabolism and cell differentiation in adipocytes. In obesity, adipose lipin-1 mRNA expression is decreased and positively correlated with systemic insulin sensitivity. Amelioration of the lipin-1 depletion might be improved dysmetabolism. Although some cytokines such as TNF-α and interleukin-1β reduces adipose lipin-1 expression, the mechanism of decreased adipose lipin-1 expression in obesity remains unclear. Recently, endoplasmic reticulum (ER) stress is implicated in the pathogenesis of obesity. Here we investigated the role of ER stress on the lipin-1 expression in 3T3-L1 adipocytes. We demonstrated that lipin-1 expression was suppressed by the treatment with ER stress inducers (tunicamycin and thapsigargin) at transcriptional level. We also showed that constitutive lipin-1 expression could be maintained by peroxisome proliferator-activated receptor-γ in 3T3-L1 adipocytes. Activation of peroxisome proliferator-activated receptor-γ recovered the ER stress-induced lipin-1 suppression. These results suggested that ER stress might be involved in the pathogenesis of obesity through lipin-1 depletion.

  6. Ginkgolide C Suppresses Adipogenesis in 3T3-L1 Adipocytes via the AMPK Signaling Pathway

    Directory of Open Access Journals (Sweden)

    Chian-Jiun Liou

    2015-01-01

    Full Text Available Ginkgolide C, isolated from Ginkgo biloba leaves, is a flavone reported to have multiple biological functions, from decreased platelet aggregation to ameliorating Alzheimer disease. The study aim was to evaluate the antiadipogenic effect of ginkgolide C in 3T3-L1 adipocytes. Ginkgolide C was used to treat differentiated 3T3-L1 cells. Cell supernatant was collected to assay glycerol release, and cells were lysed to measure protein and gene expression related to adipogenesis and lipolysis by western blot and real-time PCR, respectively. Ginkgolide C significantly suppressed lipid accumulation in differentiated adipocytes. It also decreased adipogenesis-related transcription factor expression, including peroxisome proliferator-activated receptor and CCAAT/enhancer-binding protein. Furthermore, ginkgolide C enhanced adipose triglyceride lipase and hormone-sensitive lipase production for lipolysis and increased phosphorylation of AMP-activated protein kinase (AMPK, resulting in decreased activity of acetyl-CoA carboxylase for fatty acid synthesis. In coculture with an AMPK inhibitor (compound C, ginkgolide C also improved activation of sirtuin 1 and phosphorylation of AMPK in differentiated 3T3-L1 cells. The results suggest that ginkgolide C is an effective flavone for increasing lipolysis and inhibiting adipogenesis in adipocytes through the activated AMPK pathway.

  7. Can symptomatic acromioclavicular joints be differentiated from asymptomatic acromioclavicular joints on 3-T MR imaging?

    Science.gov (United States)

    Choo, Hye Jung; Lee, Sun Joo; Kim, Jung Han; Cha, Seong Sook; Park, Young Mi; Park, Ji Sung; Lee, Jun Woo; Oh, Minkyung

    2013-04-01

    To evaluate retrospectively whether symptomatic acromioclavicular joints can be differentiated from asymptomatic acromioclavicular joints on 3-T MR imaging. This study included 146 patients who underwent physical examination of acromioclavicular joints and 3-T MR imaging of the shoulder. Among them, 67 patients showing positive results on physical examination were assigned to the symptomatic group, whereas 79 showing negative results were assigned to the asymptomatic group. The following MR findings were compared between the symptomatic and asymptomatic groups: presence of osteophytes, articular surface irregularity, subchondral cysts, acromioclavicular joint fluid, subacromial fluid, subacromial bony spurs, joint capsular distension, bone edema, intraarticular enhancement, periarticular enhancement, superior and inferior joint capsular distension degree, and joint capsular thickness. The patients were subsequently divided into groups based on age (younger, older) and the method of MR arthrography (direct MR arthrography, indirect MR arthrography), and all the MR findings in each subgroup were reanalyzed. The meaningful cutoff value of each significant continuous variable was calculated using receiver operating characteristic analysis. The degree of superior capsular distension was the only significant MR finding of symptomatic acromioclavicular joints and its meaningful cutoff value was 2.1mm. After subgroup analyses, this variable was significant in the older age group and indirect MR arthrography group. On 3-T MR imaging, the degree of superior joint capsular distension might be a predictable MR finding in the diagnosis of symptomatic acromioclavicular joints. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  8. Comparison of 3 T and 7 T MRI clinical sequences for ankle imaging

    Energy Technology Data Exchange (ETDEWEB)

    Juras, Vladimir, E-mail: vladimir.juras@meduniwien.ac.at [Medical University of Vienna, Department of Radiology, Vienna General Hospital, Waeringer Guertel 18-20, A-1090 Vienna (Austria); Slovak Academy of Sciences, Institute of Measurement Science, Dubravska cesta 9, 84104 Bratislava (Slovakia); Welsch, Goetz, E-mail: welsch@bwh.harvard.edu [Medical University of Vienna, Department of Radiology, Vienna General Hospital, Waeringer Guertel 18-20, A-1090 Vienna (Austria); Baer, Peter, E-mail: baerpeter@siemens.com [Siemens Healthcare, Richard-Strauss-Strasse 76, D81679 Munich (Germany); Kronnerwetter, Claudia, E-mail: claudia.kronnerwetter@meduniwien.ac.at [Medical University of Vienna, Department of Radiology, Vienna General Hospital, Waeringer Guertel 18-20, A-1090 Vienna (Austria); Fujita, Hiroyuki, E-mail: hiroyuki.fujita@qualedyn.com [Quality Electrodynamics, LCC, 777 Beta Dr, Cleveland, OH 44143-2336 (United States); Trattnig, Siegfried, E-mail: siegfried.trattnig@meduniwien.ac.at [Medical University of Vienna, Department of Radiology, Vienna General Hospital, Waeringer Guertel 18-20, A-1090 Vienna (Austria)

    2012-08-15

    The purpose of this study was to compare 3 T and 7 T signal-to-noise and contrast-to noise ratios of clinical sequences for imaging of the ankles with optimized sequences and dedicated coils. Ten healthy volunteers were examined consecutively on both systems with three clinical sequences: (1) 3D gradient-echo, T{sub 1}-weighted; (2) 2D fast spin-echo, PD-weighted; and (3) 2D spin-echo, T{sub 1}-weighted. SNR was calculated for six regions: cartilage; bone; muscle; synovial fluid; Achilles tendon; and Kager's fat-pad. CNR was obtained for cartilage/bone, cartilage/fluid, cartilage/muscle, and muscle/fat-pad, and compared by a one-way ANOVA test for repeated measures. Mean SNR significantly increased at 7 T compared to 3 T for 3D GRE, and 2D TSE was 60.9% and 86.7%, respectively. In contrast, an average SNR decrease of almost 25% was observed in the 2D SE sequence. A CNR increase was observed in 2D TSE images, and in most 3D GRE images. There was a substantial benefit from ultra high-field MR imaging of ankles with routine clinical sequences at 7 T compared to 3 T. Higher SNR and CNR at ultra-high field MR scanners may be useful in clinical practice for ankle imaging. However, carefully optimized protocols and dedicated extremity coils are necessary to obtain optimal results.

  9. Traditional Herbal Formula Oyaksungi-San Inhibits Adipogenesis in 3T3-L1 Adipocytes

    Directory of Open Access Journals (Sweden)

    Sae-Rom Yoo

    2015-01-01

    Full Text Available Background. Oyaksungi-san (OYSGS is a herbal formula that has been used for treating cardiovascular diseases in traditional Asian medicine. Here, we investigated the antiadipogenic effect of OYSGS extract in 3T3-L1 adipose cells. Methods. 3T3-L1 preadipocytes were differentiated into adipocytes with or without OYSGS. After differentiation, we measured Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH activity, leptin production, mRNA, and protein levels of adipogenesis-related factors. Results. OYSGS extract dramatically inhibited intracellular lipid accumulation in the differentiated adipocytes. It also significantly suppressed the (GPDH activity, triglyceride (TG content, and leptin production by reducing the expression of adipogenesis-related genes including lipoprotein lipase, fatty acid binding protein 4, CCAAT/enhancer-binding protein-alpha (C/EBP-α, and peroxisome proliferator-activated receptor gamma (PPAR-γ. Furthermore, OYSGS clearly enhanced phosphorylation of AMP-activated protein kinase (AMPK as well as its substrate acetyl CoA (ACC carboxylase. Conclusions. Our results demonstrate that OYSGS negatively controls TG accumulation in 3T3-L1 adipocytes. We suggest antiadipogenic activity of OYSGS and its potential benefit in preventing obesity.

  10. Evaluation of cytotoxicity and gelatinases activity in 3T3 fibroblast cell by root repair materials

    Directory of Open Access Journals (Sweden)

    Varol Basak

    2016-09-01

    Full Text Available The aim of this study was to investigate the effects of calcium silicate-based products on cytotoxicity in the 3T3 fibroblast and gelatinolytic activity of matrix metalloproteinases (MMPs. 3T3 fibroblasts were incubated directly with Ortho Mineral trioxide aggregate (MTA, BioAggregate, Biodentine, MTA Plus, MTA Angelus and MTA Cerkamed for 24 hours and seven days. The cytotoxicity was determined using an MTT assay. Supernatants were collected to determine MMP-2 and MMP-9. Data were analysed using IBM SPSS 22. Seventh day extracts of Ortho MTA and Biodentine showed reduced cell viability. Specific characterization of MMPs in cell culture demonstrated that MMP-2 (62 kPa in the cell culture supernatants by gelatin zymography showed induced expression in four out of seven groups by 3T3 cells. No MMP-9 expression was observed. The cytotoxicity of materials revealed a significant difference in cell viability between the groups on the first and seventh days. The results of this study revealed minor cytotoxic effects for Ortho MTA and Biodentine. This study suggests that endodontic sealers induced production of MMP-2. MMP-9 might be expressed in small amounts when compared with MMP-2.

  11. Análise de interações medicamentosas e alimentares em pacientes com AIDS em uso da TARV associada à terapia de resgate

    Directory of Open Access Journals (Sweden)

    Adriana Antônia Cruz Furini

    2016-07-01

    Full Text Available A combinação de antirretrovirais da terapia de resgate na Síndrome da Imunodeficiência Adquirida aumenta os riscos de interações medicamentosas e alimentares. Este estudo teve como objetivo analisar o risco de interações medicamentosas e alimentares no uso da terapia antirretroviral com medicamentos do resgate por 40 pacientes com AIDS de um Hospital quaternário da região Noroeste paulista. Trata-se de um estudo exploratório-descritivo de abordagem quantitativa e qualitativa, realizado no período entre2012 e 2013. Os pacientes foram entrevistados através de questionário elaborado pela equipe multidisciplinar de saúde. As entrevistas foram realizadas pela equipe de farmacêuticos. As interações foram avaliadas por bases de dados informatizados. O esquema composto por darunavir, enfuvirtida, lamivudina, raltegravir e ritonavir foi o mais utilizado (32,5% pelos pacientes. Os antirretrovirais que mais apresentaram risco de interações alimentares foram ritonavir, darunavir e tenofovir, em 31 (77,5%, 29 (72,5% e 21 (52,5% pacientes, respectivamente. Foram identificados 87 tipos de potenciais interações medicamentosas, e sete foram classificadas como de nível maior. Neste estudo todas as prescrições apresentaram riscos para interações medicamentosas e demonstraram a importância do monitoramento pela equipe multidisciplinar. Contudo, a maioria das interações medicamentosas foi benéfica, decorrentes da potencialização do efeito terapêutico em consequência do sinergismo entre os fármacos da terapia antirretroviral. A interação entre farmacêuticos e prescritores pode contribuir para a farmacoterapia racional e segura do paciente.Palavras-chave: Síndrome de Imunodeficiência Adquirida. HIV. Antirretrovirais. Interações de Medicamentos. Interações Alimento-Droga. ABSTRACTAnalysis of drug and food interactions in AIDS patients in use of ART associated with rescue therapyThe combination of antiretroviral agents of

  12. Molecular Modeling of Acidic Treated PSTM-3T Polymer for Removal of Heavy Metal Ions by Experimental and Computational Studies

    Directory of Open Access Journals (Sweden)

    Natsagdorj Narantsogt

    2014-01-01

    Full Text Available The synthesized poly[N,N′-bis(3-silsesquioxanilpropyl-thiocarbamide] (PSTM-3T was used and the surface morphology and microstructure of it were analyzed by scanning electron microscopy with energy dispersive spectrometer (SEM/EDS. The molecular structure change of the PSTM-3T polymer of the PSTM-3T after treatment by acidic solution with different pHs was revealed using FT-IR experiments and ab initio calculations with density functional theory method. The sorption efficiency of the heavy metal ions depends on the molecular structure change of PSTM-3T after treatment of different pH aqueous solutions. After the treatment of acidic solution (pH = 2 of PSTM-3T, the polymer formed the tautomer state to increase the sorption efficiency for chromate ion. For the increment of pH value for acidic solution, the PSTM-3T polymer was dissociated to increase the sorption efficiency for copper ion.

  13. [Evaluation of Artificial Hip Joint with Radiofrequency Heating Issues during MRI Examination: A Comparison between 1.5 T and 3 T].

    Science.gov (United States)

    Yamazaki, Masaru; Ideta, Takahiro; Kudo, Sadahiro; Nakazawa, Masami

    2016-06-01

    In magnetic resonance imaging (MRI), when radiofrequency (RF) is irradiated to a subject with metallic implant, it can generate heat by RF irradiation. Recently 3 T MRI scanner has spread widely and imaging for any regions of whole body has been conducted. However specific absorption rate (SAR) of 3 T MRI becomes approximately four times as much as the 1.5 T, which can significantly affect the heat generation of metallic implants. So, we evaluated RF heating of artificial hip joints in different shapes and materials in 1.5 T and 3 T MRI. Three types of artificial hip joints made of stainless alloy, titanium alloy and cobalt chrome alloy were embedded in the human body-equivalent phantom respectively and their temperature change were measured for twenty minutes by 1.5 T and 3 T MRI. The maximum temperature rise was observed at the bottom head in all of three types of artificial hip joints, the rise being 12°C for stainless alloy, 11.9°C for titanium alloy and 6.1°C for cobalt chrome alloy in 1.5 T. The temperature rise depended on SAR and the increase of SAR had a good linear relationship with the temperature rise. It was found from the result that the RF heating of metallic implants can take place in various kinds of material and the increase of SAR has a good linear relationship with the temperature rise. This experience shows that reduction of SAR can decrease temperature of metallic implants.

  14. Perception of matching and conflicting audiovisual speech in dyslexic and fluent readers: an fMRI study at 3 T.

    Science.gov (United States)

    Pekkola, Johanna; Laasonen, Marja; Ojanen, Ville; Autti, Taina; Jääskeläinen, Iiro P; Kujala, Teija; Sams, Mikko

    2006-02-01

    We presented phonetically matching and conflicting audiovisual vowels to 10 dyslexic and 10 fluent-reading young adults during "clustered volume acquisition" functional magnetic resonance imaging (fMRI) at 3 T. We further assessed co-variation between the dyslexic readers' phonological processing abilities, as indexed by neuropsychological test scores, and BOLD signal change within the visual cortex, auditory cortex, and Broca's area. Both dyslexic and fluent readers showed increased activation during observation of phonetically conflicting compared to matching vowels within the classical motor speech regions (Broca's area and the left premotor cortex), this activation difference being more extensive and bilateral in the dyslexic group. The between-group activation difference in conflicting > matching contrast reached significance in the motor speech regions and in the left inferior parietal lobule, with dyslexic readers exhibiting stronger activation than fluent readers. The dyslexic readers' BOLD signal change co-varied with their phonological processing abilities within the visual cortex and Broca's area, and to a lesser extent within the auditory cortex. We suggest these findings as reflecting dyslexic readers' greater use of motor-articulatory and visual strategies during phonetic processing of audiovisual speech, possibly to compensate for their difficulties in auditory speech perception.

  15. Exploring cartilage damage in gout using 3-T MRI: distribution and associations with joint inflammation and tophus deposition

    Energy Technology Data Exchange (ETDEWEB)

    Popovich, I. [University of Auckland, Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, 85 Park Road, Grafton, Auckland (New Zealand); Dalbeth, N. [University of Auckland, Department of Medicine, Auckland (New Zealand); Auckland District Health Board, Department of Rheumatology, Auckland (New Zealand); Doyle, A.; Reeves, Q. [Auckland District Health Board, Department of Radiology, Auckland (New Zealand); McQueen, F.M. [University of Auckland, Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, 85 Park Road, Grafton, Auckland (New Zealand); Auckland District Health Board, Department of Rheumatology, Auckland (New Zealand)

    2014-07-15

    Few imaging studies have investigated cartilage in gout. Magnetic resonance imaging (MRI) can image cartilage damage and also reveals other features of gouty arthropathy. The objective was to develop and validate a system for quantifying cartilage damage in gout. 3-T MRI scans of the wrist were obtained in 40 gout patients. MRI cartilage damage was quantified using an adaptation of the radiographic Sharp van der Heijde score. Two readers scored cartilage loss at 7 wrist joints: 0 (normal), 1 (partial narrowing), 2 (complete narrowing) and concomitant osteoarthritis was recorded. Bone erosion, bone oedema and synovitis were scored (RAMRIS) and tophi were assessed. Correlations between radiographic and MRI cartilage scores were investigated, as was the reliability of the MRI cartilage score and its associations. The GOut MRI Cartilage Score (GOMRICS) was highly correlated with the total Sharp van der Heijde (SvdH) score and the joint space narrowing component (R = 0.8 and 0.71 respectively, p < 0.001). Reliability was high (intraobserver, interobserver ICCs = 0.87 [0.57-0.97], 0.64 [0.41-0.79] respectively), and improved on unenhanced scans; interobserver ICC = 0.82 [0.49-0.95]. Cartilage damage was predominantly focal (82 % of lesions) and identified in 40 out of 280 (14 %) of joints. Cartilage scores correlated with bone erosion (R = 0.57), tophus size (R = 0.52), and synovitis (R = 0.55), but not bone oedema scores. Magnetic resonance imaging can be used to investigate cartilage in gout. Cartilage damage was relatively uncommon, focal, and associated with bone erosions, tophi and synovitis, but not bone oedema. This emphasises the unique pathophysiology of gout. (orig.)

  16. 17 CFR 240.15b7-3T - Operational capability in a Year 2000 environment.

    Science.gov (United States)

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Operational capability in a Year 2000 environment. 240.15b7-3T Section 240.15b7-3T Commodity and Securities Exchanges SECURITIES... § 240.15b7-3T Operational capability in a Year 2000 environment. (a) This section applies to...

  17. File list: NoD.EmF.05.AllAg.NIHSLASH3T3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.EmF.05.AllAg.NIHSLASH3T3 mm9 No description Embryonic fibroblast NIH/3T3 SRX475...500,SRX666257,SRX666258,SRX666259,SRX666260,SRX657828,SRX591250 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.EmF.05.AllAg.NIHSLASH3T3.bed ...

  18. File list: NoD.EmF.50.AllAg.NIHSLASH3T3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  1. MT and spillover correction for quantitative steady-state pulsed CEST-MRI at 3T using the reciprocal Z-spectrum

    CERN Document Server

    Zaiss, Moritz; Bachert, Peter

    2013-01-01

    Endogenous chemical exchange saturation transfer (CEST) effects are always diluted by competing effects such as direct water proton saturation (spillover) and macromolecular magnetization transfer (MT). This leads to T2-and MT-shine-through effects in the actual biochemical contrast of CEST. Therefore, a simple evaluation algorithm which corrects the CEST signal was searched for. By employing a recent eigenspace theory valid for spinlock and continuous wave (cw) CEST we predict that the inverse Z-spectrum is beneficial to Z-spectrum itself. Based on this we propose a new spillover- and MT-corrected magnetization transfer ratio (MTRRex) yielding Rex, the exchange dependent relaxation rate in the rotating frame. For verification, the amine proton exchange of creatine in solutions with different agar concentration was studied experimentally at clinical field strength of 3T. In contrast to the compared standard evaluation for pulsed CEST experiments, MTRasym, our approach shows no T2 or MT shine through effect. W...

  2. Ultra-low field T1 vs. T1rho at 3T and 7T: study of rotationally immobilized protein gels and animal brain tissues

    Science.gov (United States)

    Dong, Hui; Inglis, Ben; Barr, Ian; Clarke, John

    2015-03-01

    Clinical magnetic resonance imaging (MRI) machines operating in static fields of typically 1.5 T or 3 T can capture information on slow molecular dynamics utilizing the so-called T1rho technique. This technique, in which a radiofrequency (RF) spin-lock field is applied with microtesla amplitude, has been used, for example, to determine the onset time of stroke in studies on rats. The long RF pulse, however, may exceed the specific absorption rate (SAR) limit, putting subjects at risk. Ultra-low-field (ULF) MRI, based on Superconducting Quantum Interference Devices (SQUIDs), directly detects proton signals at a static magnetic field of typically 50-250 μT. Using our ULF MRI system with adjustable static field of typically 55 to 240 μT, we systematically measured the T1 and T2 dispersion profiles of rotationally immobilized protein gels (bovine serum albumin), ex vivo pig brains, and ex vivo rat brains with induced stroke. Comparing the ULF results with T1rho dispersion obtained at 3 T and 7 T, we find that the degree of protein immobilization determines the frequency-dependence of both T1 and T1rho. Furthermore, T1rho and ULF T1 show similar results for stroke, suggesting that ULF MRI may be used to image traumatic brain injury with negligible SAR. This research was supported by the Henry H. Wheeler, Jr. Brain Imaging Center and the Donaldson Trust.

  3. Interações medicamentosas potenciais entre idosos em uso dos anti-hipertensivos da Relação Nacional de Medicamentos Essenciais do Ministério da Saúde do Brasil

    Directory of Open Access Journals (Sweden)

    Pablo Mibielli

    2014-09-01

    Full Text Available O objetivo deste estudo foi estimar a prevalência de interações medicamentosas potenciais entre anti-hipertensivos e outros fármacos. Foi realizado um inquérito domiciliar com pessoas de 60 anos ou mais de idade, residentes no Rio de Janeiro, Brasil. Foram identificadas as interações medicamentosas potenciais entre os anti- hipertensivos com evidência estabelecida, provável ou suspeita e com gravidade moderada ou elevada. Foram entrevistados 577 idosos (média de idade = 72 anos, 45,2% dos quais em uso de anti-hipertensivos, sendo 31,0% deles sujeitos a interações medicamentosas potenciais. A maioria das interações foi moderadamente grave. Comparados aos demais, os sujeitos às interações medicamentosas potenciais têm chance acima de 4 vezes de usar 5 ou mais medicamentos e acima de duas vezes de ter sido hospitalizado no ano anterior. Entre os pares de interações mais frequentes, 75% produzem redução do efeito hipotensivo (65/87, o que pode resultar em baixa efetividade no controle da pressão arterial, prescrição de mais medicamentos e risco de outros efeitos adversos e de interações.

  4. Multi-session complex averaging for high resolution high SNR 3T MR visualization of ex vivo hippocampus and insula

    Science.gov (United States)

    Stamm, Aymeric; Singh, Jolene M.; Scherrer, Benoit; Afacan, Onur; Warfield, Simon K.

    2015-03-01

    The hippocampus and the insula are responsible for episodic memory formation and retrieval. Hence, visualization of the cytoarchitecture of such structures is of primary importance to understand the underpinnings of conscious experience. Magnetic Resonance Imaging (MRI) offers an opportunity to non-invasively image these crucial structures. However, current clinical MR imaging operates at the millimeter scale while these anatomical landmarks are organized into sub-millimeter structures. For instance, the hippocampus contains several layers, including the CA3-dentate network responsible for encoding events and experiences. To investigate whether memory loss is a result of injury or degradation of CA3/dentate, spatial resolution must exceed one hundred micron, isotropic, voxel size. Going from one millimeter voxels to one hundred micron voxels results in a 1000× signal loss, making the measured signal close to or even way below the precision of the receiving coils. Consequently, the signal magnitude that forms the structural images will be biased and noisy, which results in inaccurate contrast and less than optimal signal-to-noise ratio (SNR). In this paper, we propose a strategy to perform high spatial resolution MR imaging of the hippocampus and insula with 3T scanners that enables accurate contrast (no systematic bias) and arbitrarily high SNR. This requires the collection of additional repeated measurements of the same image and a proper averaging of the k-space data in the complex domain. This comes at the cost of additional scan time, but long single-session scan times are not practical for obvious reasons. Hence, we also develop an approach to combine k-space data from multiple sessions, which enables the total scan time to be split into arbitrarily short sessions, where the patient is allowed to move and rest in-between. For validation, we hereby illustrate our multi-session complex averaging strategy by providing high spatial resolution 3T MR visualization

  5. Potential Heating Effect in the Gravid Uterus by Using 3-T MR Imaging Protocols: Experimental Study in Miniature Pigs.

    Science.gov (United States)

    Cannie, Mieke M; De Keyzer, Frederik; Van Laere, Sigrid; Leus, Astrid; de Mey, Johan; Fourneau, Catherine; De Ridder, Filip; Van Cauteren, Toon; Willekens, Inneke; Jani, Jacques C

    2016-06-01

    Purpose To determine the changes in temperature within the gravid miniature pig uterus during magnetic resonance (MR) imaging at 3 T. Materials and Methods The study received ethics committee approval for animal experimentation. Fiber-optic temperature sensors were inserted into the fetal brain, abdomen, bladder, and amniotic fluid of miniature pigs (second trimester, n = 2; third trimester, n = 2). In the first trimester (n = 2), the sensors were inserted only into the amniotic fluid (three sacs per miniature pig, for a total of six sacs). Imaging was performed with a 3-T MR imager by using different imaging protocols in a random order for animal, each lasting approximately 15 minutes. The first regimen consisted of common sequences used for human fetal MR examination, including normal specific absorption rate (SAR). The second regimen consisted of five low-SAR sequences, for which three gradient-echo sequences were interspersed with two diffusion-weighted imaging series. Finally, a high-SAR regimen maximized the radiofrequency energy deposition (constrained by the 2-W per kilogram of body weight SAR limitations) by using five single-shot turbo spin-echo sequences. Differences in temperature increases between the three regimens and between the three trimesters were evaluated by using one-way analysis of variance. The maximum cumulative temperature increase over 1 hour was also evaluated. Results Low-SAR regimens resulted in the lowest temperature increase (mean ± standard deviation, -0.03°C ± 0.20), normal regimens resulted in an intermediate increase (0.31°C ± 0.21), and high-SAR regimens resulted in the highest increase (0.56°C ± 0.20) (P lead to temperature increases above 1°C if imaging time is kept below 30 minutes. Longer imaging time, especially with high-SAR regimens, can lead to an increase of 2.5°C. (©) RSNA, 2015 Online supplemental material is available for this article.

  6. A resistin binding peptide selected by phage display inhibits 3T3-L1 preadipocyte differentiation

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Background Resistin, a newly discovered cysteine-rich hormone secreted mainly by adipose tissues, has been proposed to form a biochemical link between obesity and type 2 diabetes. However, the resistin receptor has not yet been identified. This study aimed to identify resistin binding proteins/receptor.Methods Three cDNA fragments with the same 11 bp 5' sequence were found by screening a cDNA phage display library of rat multiple tissues. As the reading frames of the same 11 bp 5' sequence were interrupted by a TGA stop codon, plaque lift assay was consequently used to prove the readthrough phenomenon. The stop codon in the same 11 bp 5' sequence was replaced by tryptophan, and the binding activity of the coded peptide [AWIL, which was designated as resistin binding peptide (RBP)] with resistin was identified by the confocal microscopy technique and the affinity chromatography experiment. pDual GC-resistin and pDual GC-resistin binding peptide were co-transfected into 3T3-L1 cells to confirm the function of resistin binding peptide.Results Three cDNA fragments with the same 11 bp 5' sequence were found. The TGA stop codon in reading frames of the same 11 bp 5' sequence was proved to be readthroughed. The binding activity of RBP with resistin was consequently identified. The expression of the resistin binding peptide in 3T3-L1 preadipocytes expressing pDual GC-resistin significantly inhibited the adipogenic differentiation.Conclusion RBP could effectively rescue the promoted differentiation of resistin overxepressed 3T3-L1 preadipocyte.

  7. Nebivolol stimulates mitochondrial biogenesis in 3T3-L1 adipocytes

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Chenglin; Chen, Dongrui; Xie, Qihai [State Key Laboratory of Medical Genomics, Shanghai Key Laboratory of Vascular Biology, Department of Hypertension, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025 (China); Yang, Ying, E-mail: yangying_sh@yahoo.com [Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025 (China); Shen, Weili, E-mail: weili_shen@hotmail.com [State Key Laboratory of Medical Genomics, Shanghai Key Laboratory of Vascular Biology, Department of Hypertension, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025 (China)

    2013-08-16

    Highlights: •Nebivolol may act as a partial agonist of β3-adrenergic receptor (AR). •Nebivolol stimulates mitochondrial DNA replication and protein expression. •Nebivolol promotes mitochondrial synthesis via activation of eNOS by β3-AR. -- Abstract: Nebivolol is a third-generation β-adrenergic receptor (β-AR) blocker with additional beneficial effects, including the improvement of lipid and glucose metabolism in obese individuals. However, the underlying mechanism of nebivolol’s role in regulating the lipid profile remains largely unknown. In this study, we investigated the role of nebivolol in mitochondrial biogenesis in 3T3-L1 adipocytes. Exposure of 3T3-L1 cells to nebivolol for 24 h increased mitochondrial DNA copy number, mitochondrial protein levels and the expression of transcription factors involved in mitochondrial biogenesis, including PPAR-γ coactivator-1α (PGC-1α), Sirtuin 3 (Sirt3), mitochondrial transcription factor A (Tfam) and nuclear related factor 1 (Nrf1). These changes were accompanied by an increase in oxygen consumption and in the expression of genes involved in fatty acid oxidation and antioxidant enzymes in 3T3-L1 adipocytes, including nebivolol-induced endothelial nitric oxide synthase (eNOS), as well as an increase in the formation of cyclic guanosine monophosphate (cGMP). Pretreatment with NG-nitro-L-arginine methyl ester (l-NAME) attenuated nebivolol-induced mitochondrial biogenesis, as did the soluble guanylate cyclase inhibitor, ODQ. Treatment with nebivolol and β3-AR blocker SR59230A markedly attenuated PGC-1α, Sirt3 and manganese superoxide dismutase (MnSOD) protein levels in comparison to treatment with nebivolol alone. These data indicate that the mitochondrial synthesis and metabolism in adipocytes that is promoted by nebivolol is primarily mediated through the eNOS/cGMP-dependent pathway and is initiated by the activation of β3-AR receptors.

  8. The aporphine alkaloid boldine induces adiponectin expression and regulation in 3T3-L1 cells.

    Science.gov (United States)

    Yu, Bangning; Cook, Carla; Santanam, Nalini

    2009-10-01

    Adiponectin is an adipokine secreted by differentiated adipocytes. Clinical studies suggest a negative correlation between oxidative stress and adiponectin levels in patients with metabolic syndrome or cardiovascular disease. Natural compounds that can prevent oxidative stress mediated inhibition of adiponectin may be potentially therapeutic. Boldine, an aporphine alkaloid abundant in the medicinal plant Peumus boldus, is a powerful antioxidant. The current study demonstrates the effects of boldine on the expression of adiponectin and its regulators, CCAAT/enhancer binding protein-alpha (C/EBPalpha) and peroxisome proliferator-activated receptor (PPAR)-gamma, in 3T3-L1 cells. Differentiated 3T3-L1 adipocytes were exposed to either hydrogen peroxide (H(2)O(2)) (100 microM) or tumor necrosis factor-alpha (TNFalpha) (1 ng/mL) for 24 hours in the presence or absence of increasing concentrations of boldine (5-100 microM). Quantitative polymerase chain reaction showed that both the oxidants decreased the mRNA levels of adiponectin, PPARgamma, and C/EBPalpha to half of the control levels. Boldine, at all concentrations, counteracted the inhibitory effect of H(2)O(2) or TNFalpha and increased the expression of adiponectin and its regulators. The effect of boldine on adiponectin expression was biphasic, with the lower concentrations (5-25 microM) having a larger inductive effect compared to higher concentrations (50-100 microM). Boldine treatment alone in the absence of H(2)O(2) or TNFalpha was also able to induce adiponectin at the inductive phase of adipogenesis. Peroxisome proliferator response element-luciferase promoter transactivity analysis showed that boldine interacts with the PPAR response element and could potentially modulate PPAR responsive genes. Our results indicate that boldine is able to modulate the expression of adiponectin and its regulators in 3T3-L1 cells and has the potential to be beneficial in obesity-related cardiovascular disease.

  9. Tea catechins modulate the glucose transport system in 3T3-L1 adipocytes.

    Science.gov (United States)

    Ueda, Manabu; Furuyashiki, Takashi; Yamada, Kayo; Aoki, Yukiko; Sakane, Iwao; Fukuda, Itsuko; Yoshida, Ken-Ichi; Ashida, Hitoshi

    2010-11-01

    In this study, we investigated the effects of tea catechins on the translocation of glucose transporter (GLUT) 4 in 3T3-L1 adipocytes. We found that the ethyl acetate fraction of green tea extract, containing abundant catechins, most decreased insulin-induced glucose uptake activity in 3T3-L1 cells. When the cells were treated with 50 μM catechins in the absence or presence of insulin for 30 min, nongallate-type catechins increased glucose uptake activity without insulin, whereas gallate-type catechins decreased insulin-induced glucose uptake activity. (-)-Epicatechin (EC) and (-)-epigallocatechin (EGC), nongallate-type catechins, increased glucose uptake activity in the dose- and time-dependent manner, whereas (-)-catechin 3-gallate (Cg) and (-)-epigallocatechin 3-gallate (EGCg), gallate-type catechins, decreased insulin-induced glucose uptake activity in the dose- and time-dependent manner. When the cells were treated with 50 μM catechins for 30 min, EC and EGC promoted GLUT4 translocation, whereas Cg and EGCg decreased the insulin-induced translocation in the cells. EC and EGC increased phosphorylation of PKCλ/ζ without phosphorylation of insulin receptor (IR) and Akt. Wortmannin and LY294002, inhibitors for phosphatidylinositol 3'-kinase (PI3K), decreased EC- and EGC-induced glucose uptake activity in the cells. Cg and EGCg decreased phosphorylation of PKCλ/ζ in the presence of insulin without affecting insulin-induced phosphorylation of IR, and Akt. Therefore, EC and EGC promote the translocation of GLUT4 through activation of PI3K, and Cg and EGCg inhibit insulin-induced translocation of GLUT4 by the insulin signaling pathway in 3T3-L1 cells.

  10. Lysophosphatidic acid induces chemotaxis in MC3T3-E1 osteoblastic cells

    Energy Technology Data Exchange (ETDEWEB)

    Masiello, Lisa M.; Fotos, Joseph S.; Galileo, Deni S.; Karin, Norm J.

    2006-07-01

    Lysophosphatidic acid (LPA) is a bioactive lipid that has pleiotropic effects on a variety of cell types and enhances the migration of endothelial and cancer cells, but it is not known if this lipid can alter osteoblast motility. We performed transwell migration assays using MC3T3-E1 osteoblastic cells and found LPA to be a potent chemotactic agent. Quantitative time-lapse video analysis of osteoblast migration after wounds were introduced into cell monolayers indicated that LPA stimulated both migration velocity and the average migration distance per cell. LPA also elicited substantial changes in cell shape and actin cytoskeletal structure; lipid-treated cells contained fewer stress fibers and displayed long membrane processes that were enriched in F-actin. Quantitative RT-PCR analysis showed that MC3T3-E1 cells express all four known LPA-specific G protein-coupled receptors (LPA1-LPA4) with a relative mRNA abundance of LPA1 > LPA4 > LPA2 >> LPA3. LPA-induced changes in osteoblast motility and morphology were antagonized by both pertussis toxin and Ki16425, a subtype-specific blocker of LPA1 and LPA3 receptor function. Cell migration in many cell types is linked to changes in intracellular Ca2+. Ki16425 also inhibited LPA-induced Ca2+ signaling in a dose-dependent manner, suggesting a link between LPA-induced Ca2+ transients and osteoblast chemotaxis. Our data show that LPA stimulates MC3T3-E1 osteoblast motility via a mechanism that is linked primarily to the G protein-coupled receptor LPA1.

  11. Effects of bovine colostral ultrafiltrates on growth and differentiation of 3T3-L1 preadipocytes.

    Science.gov (United States)

    Lee, Seong-Ho; Hossner, Kim L

    2002-12-01

    This study was designed to compare the effects of whole and size-fractionated bovine colostrum with bovine calf serum (BCS) on the growth and differentiation of 3T3-L1 fibroblasts. High (HMW) and low (LMW)-molecular-mass ultrafiltrate fractions of colostrum were prepared from defatted colostrum (COL) by diafiltration through membranes with a molecular-mass cut-off of 30 kDa. Incorporation of [(3)H]thymidine into the cells was used as a reflection of DNA synthesis/cell proliferation. The growth-promoting activity of LMW was 2.3- and 2.5-fold higher than COL and HMW, respectively (P insulin-like growth factor (IGF)-I, IGF-II and platelet-derived growth factor AB stimulated 3T3-L1 cells, antibodies to these factors did not inhibit the LMW effects. The LMW fraction was about twice as effective as COL and HMW in stimulating differentiation of the cells into adipocytes, but maximal differentiation was only 60% of that seen with 10% fetal bovine serum (FBS). Treatment with COL, HMW, IGF-I and insulin induced peroxisome-proliferator-activated receptor gamma RNA, but levels were about half of that with 10% FBS treatment and LMW induction was 80% of FBS. Low amounts of leptin mRNA were detected in adipocytes and abundance did not differ between treatments with BCS, hormones or COL fractions. This study showed that bovine colostral LMW stimulated the growth and differentiation of 3T3-L1 preadipocytes and may be a useful serum substitute to support the growth of these cells.

  12. Conventional kinesin KIF5B mediates adiponectin secretion in 3T3-L1 adipocytes

    Energy Technology Data Exchange (ETDEWEB)

    Cui, Ju, E-mail: juzi.cui@gmail.com [The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Beijing (China); Pang, Jing; Lin, Ya-Jun; Jiang, Ping; Gong, Huan [The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Beijing (China); Wang, Zai [Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing (China); Li, Jian; Cai, Jian-Ping [The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Beijing (China); Huang, Jian-Dong, E-mail: jdhuang@hku.hk [School of Biomedical Sciences and Shenzhen Institute of Research and Innovation, The University of Hong Kong, Pokfulam (Hong Kong); The Centre for Synthetic Biology Engineering Research, Shenzhen Institutes of Advanced Technology, Shenzhen (China); Zhang, Tie-Mei, E-mail: tmzhang126@126.com [The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Beijing (China)

    2016-08-05

    Insulin stimulates adiponectin secretion and glucose transporter type 4 (GLUT4) translocation in adipocyte to regulate metabolism homeostasis. Similar to GLUT4 translocation, intracellular trafficking and release of adiponectin in adipocytes relies on the trans-Golgi network and endosomal system. Recent studies show that the heavy chain of conventional kinesin (KIF5B) mediates GLUT4 translocation in murine 3T3-L1 adipocytes, however, the motor machinery involved in mediating intracellular trafficking and release of adiponectin is unknown. Here, we examined the role of KIF5B in the regulation of adiponectin secretion. The KIF5B level was up-regulated during 3T3-L1 adipogenesis. This increase in cytosolic KIF5B was synchronized with the induction of adiponectin. Endogenous KIF5B and adiponectin were partially colocalized at the peri-nuclear and cytosolic regions. In addition, adiponectin-containing vesicles were co-immunoprecipitated with KIF5B. Knockdown of KIF5B resulted in a marked inhibition of adiponectin secretion and overexpression of KIF5B enhanced adiponectin release, whereas leptin secretion was not affected by changes in KIF5B expression. These data suggest that the secretion of adiponectin, but not leptin, is dependent on functional KIF5B. - Highlights: • The KIF5B level was up regulated during 3T3-L1 adipogenesis. • Endogenous KIF5B and adiponectin were partially colicalized. • Adiponectin-containing vesicles were co-immunoprecipitated with KIF5B. • The secretion of adiponectin, but not leptin, is dependent on functional KIF5B.

  13. Evaluation of chylomicron effect on ASP production in 3T3-L1 adipocytes.

    Science.gov (United States)

    Gao, Ying; Gauvreau, Danny; Cui, Wei; Lapointe, Marc; Paglialunga, Sabina; Cianflone, Katherine

    2011-02-01

    In the past few years, there has been increasing interest in the production and physiological role of acylation-stimulating protein (ASP), identical to C3adesArg, a product of the alternative complement pathway generated through C3 cleavage. Recent studies in C3 (-/-) mice that are ASP deficient have demonstrated a role for ASP in postprandial triglyceride clearance and fat storage. The aim of the present study was to establish a cell model and sensitive ELISA assay for the evaluation of ASP production using 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into adipocytes, then cultured in different media such as serum-free (SF), Dulbecco's modified Eagle's medium (DMEM)/F12 + 10% fetal calf serum (FBS), and at varying concentrations of chylomicrons and insulin + chylomicrons up to 48 h. ASP production in SF and DMEM/F12 + 10% FBS was compared. Chylomicrons stimulated ASP production in a concentration- and time-dependent manner. By contrast, chylomicron treatment had no effect on the production of C3, the precursor protein of ASP, which was constant over 48 h. Addition of insulin (100 nM) to a low-dose of chylomicrons (100 µg TG/ml) significantly increased ASP production compared with chylomicrons alone at 48 h (P ASP to C3 remained constant, indicating no change in the ratio of C3 cleaved to generate ASP. This study demonstrated that 3T3-L1 adipocyte is a useful model for the evaluation of C3 secretion and ASP production by using a sensitive mouse-specific ELISA assay. The stimulation of ASP production with chylomicrons demonstrates a physiologically relevant response, and provides a strategy for further studies on ASP production and function.

  14. Hydrogen sulfide promotes adipogenesis in 3T3L1 cells.

    Directory of Open Access Journals (Sweden)

    Chin-Yi Tsai

    Full Text Available The effect of hydrogen sulfide (H2S on differentiation of 3T3L1-derived adipocytes was examined. Endogenous H2S was increased after 3T3L1 differentiation. The expression of the H2S-synthesising enzymes, cystathionine γ-lyase (CSE, cystathionine β-synthase (CBS and 3-mercaptopyruvate sulfurtransferase (3-MST, was increased in a time-dependent manner during 3T3L1 differentiation. Expression of genes associated with adipogenesis related genes including fatty acid binding protein 4 (FABP4/aP2, a key regulator of this process, was increased by GYY4137 (a slow-releasing H2S donor compound and sodium hydrosulfide (NaHS, a classical H2S donor but not by ZYJ1122 or time-expired NaHS. Furthermore expression of these genes were reduced by aminooxyacetic acid (AOAA, CBS inhibitor, DL-propargylglycine (PAG, CSE inhibitor as well as by CSE small interference RNA (siCSE and siCBS. The size and number of lipid droplets in mature adipocytes was significantly increased by both GYY4137 and NaHS, which also impaired the ability of CL316,243 (β3-agonist to promote lipolysis in these cells. In contrast, AOAA and PAG had the opposite effect. Taken together, we show that the H2S-synthesising enzymes CBS, CSE and 3-MST are endogenously expressed during adipogenesis and that both endogenous and exogenous H2S modulate adipogenesis and adipocyte maturation.

  15. A interação na pedagogia a distância: a visão de tutoria e estudantes

    Directory of Open Access Journals (Sweden)

    Erlinda Martins Batista

    2016-05-01

    Full Text Available This study shares the results of a survey that sought to analyze the process of interaction in a Virtual Learning Environment – VLE from the perspective of students and tutors of a distance learning course of Pedagogy offered by a Brazilian public university. Based on a critical paradigm and a qualitative methodology, this article presents a discussion on the role of interaction in distance learning courses, from the viewpoints of students and tutors. The participants´ concepts of interaction were analyzed from the dialectic and socio-historical perspective, according to which interaction is established in the social relations and mediated by the educator to promote learning. The results suggest that interaction is characterized as a form of communication that takes place especially in the virtual setting, and that it has a strong unidirectional character, not so interactionist, as it occurs in only one direction, in this case from students towards tutors as far as schoolwork is concerned. It was concluded that both tutors and students need to reframe the concept of interaction, considered in the study as a process of ‘wholeness’. In the concept of ‘wholeness’, communication occurs so that the Interacting participant is wholly involved in the communication. His perception of communication is twofold, simultaneous, that is, he sees himself as whole, both in interaction and in the wholeness process. The dialogs in this process occur in a historical, social and dialectical way. Therefore, we support a process that goes beyond interaction, that is, wholeness to promote effective learning in DE – Distance Education. Este estudo compartilha os resultados de uma pesquisa que buscou analisar o processo de interação em ambiente virtual de aprendizagem – AVA, na visão dos estudantes, professores tutores de um curso de pedagogia a distância de uma instituição pública brasileira. Fundamentado no paradigma crítico e na metodologia

  16. Twist-3 T-odd fragmentation functions G⊥ and G˜⊥ in a spectator model

    Science.gov (United States)

    Yang, Yongliang; Lu, Zhun; Schmidt, Ivan

    2016-10-01

    We present a calculation of the twist-3 T-odd chiral-even fragmentation functions G⊥ and G˜⊥ using a spectator model. We consider the effect gluon exchange to calculate all necessary one-loop diagrams for the quark-quark and quark-gluon-quark correlation functions. We find that the gluon loops corrections generate non-zero contribution to these two fragmentation function. We numerically calculate their half-kT moments by integrating over the transverse momentum and also verify the equation of motion relation among G⊥, G˜⊥ and the Collins function.

  17. Nih-3T3 Fibroblast Studied by Fourier Transform Infrared Spectroscopy

    CERN Document Server

    Iovenitti, Marco

    2009-01-01

    In this work I present the study of the behaviour response of mouse fibroblasts NIH-3T3 under UVB radiation using Fourier transform infrared spectroscopy (FT-IR), the preferred method of infrared spectroscopy. FT-IR, in fact, it is convenient to study molecular cell processes because it has the potential to provide the identification of the vibrational modes of some of the major compounds (lipid, proteins and nucleic acids) without being invasive in the biomaterials. The results show that apoptotic process is induced by UVB radiation.

  18. Pathogenic conversion of Foxp3+ T cells into TH17 cells in autoimmune arthritis.

    Science.gov (United States)

    Komatsu, Noriko; Okamoto, Kazuo; Sawa, Shinichiro; Nakashima, Tomoki; Oh-hora, Masatsugu; Kodama, Tatsuhiko; Tanaka, Sakae; Bluestone, Jeffrey A; Takayanagi, Hiroshi

    2014-01-01

    Autoimmune diseases often result from an imbalance between regulatory T (Treg) cells and interleukin-17 (IL-17)-producing T helper (TH17) cells; the origin of the latter cells remains largely unknown. Foxp3 is indispensable for the suppressive function of Treg cells, but the stability of Foxp3 has been under debate. Here we show that TH17 cells originating from Foxp3(+) T cells have a key role in the pathogenesis of autoimmune arthritis. Under arthritic conditions, CD25(lo)Foxp3(+)CD4(+) T cells lose Foxp3 expression (herein called exFoxp3 cells) and undergo transdifferentiation into TH17 cells. Fate mapping analysis showed that IL-17-expressing exFoxp3 T (exFoxp3 TH17) cells accumulated in inflamed joints. The conversion of Foxp3(+)CD4(+) T cells to TH17 cells was mediated by synovial fibroblast-derived IL-6. These exFoxp3 TH17 cells were more potent osteoclastogenic T cells than were naive CD4(+) T cell-derived TH17 cells. Notably, exFoxp3 TH17 cells were characterized by the expression of Sox4, chemokine (C-C motif) receptor 6 (CCR6), chemokine (C-C motif) ligand 20 (CCL20), IL-23 receptor (IL-23R) and receptor activator of NF-κB ligand (RANKL, also called TNFSF11). Adoptive transfer of autoreactive, antigen-experienced CD25(lo)Foxp3(+)CD4(+) T cells into mice followed by secondary immunization with collagen accelerated the onset and increased the severity of arthritis and was associated with the loss of Foxp3 expression in the majority of transferred T cells. We observed IL-17(+)Foxp3(+) T cells in the synovium of subjects with active rheumatoid arthritis (RA), which suggests that plastic Foxp3(+) T cells contribute to the pathogenesis of RA. These findings establish the pathological importance of Foxp3 instability in the generation of pathogenic TH17 cells in autoimmunity.

  19. NYU3T: teaching, technology, teamwork: a model for interprofessional education scalability and sustainability.

    Science.gov (United States)

    Djukic, Maja; Fulmer, Terry; Adams, Jennifer G; Lee, Sabrina; Triola, Marc M

    2012-09-01

    Interprofessional education is a critical precursor to effective teamwork and the collaboration of health care professionals in clinical settings. Numerous barriers have been identified that preclude scalable and sustainable interprofessional education (IPE) efforts. This article describes NYU3T: Teaching, Technology, Teamwork, a model that uses novel technologies such as Web-based learning, virtual patients, and high-fidelity simulation to overcome some of the common barriers and drive implementation of evidence-based teamwork curricula. It outlines the program's curricular components, implementation strategy, evaluation methods, and lessons learned from the first year of delivery and describes implications for future large-scale IPE initiatives.

  20. Design of Low Power and Efficient Carry Select Adder Using 3-T XOR Gate

    Directory of Open Access Journals (Sweden)

    Gagandeep Singh

    2014-01-01

    Full Text Available In digital systems, mostly adder lies in the critical path that affects the overall performance of the system. To perform fast addition operation at low cost, carry select adder (CSLA is the most suitable among conventional adder structures. In this paper, a 3-T XOR gate is used to design an 8-bit CSLA as XOR gates are the essential blocks in designing higher bit adders. The proposed CSLA has reduced transistor count and has lesser power consumption as well as power-delay product (PDP as compared to regular CSLA and modified CSLA.

  1. Colchicine inhibits epidermal growth factor degradation in 3T3 cells.

    OpenAIRE

    Brown, K. D.; Friedkin, M; Rozengurt, E

    1980-01-01

    Colchicine (2 microM) did not affect the initial rate of association of 125I-labeled epidermal growth factor (125I-EGF) to Swiss 3T3 cells but continued incubation (up to 24 hr) led to an increase in cell-associated radioactivity. The effect is also produced by Colcemid, vinblastine, and podophyllotoxin but not by lumicolchicine. Disruption of microtubules with colchicine does not alter the rate of "down regulation" of EGF receptors, suggesting the binding and internalization of the factor pr...

  2. Overexpression of NYGGF4 (PID1) induces mitochondrial impairment in 3T3-L1 adipocytes.

    Science.gov (United States)

    Zhao, Yaping; Zhang, Chunmei; Chen, Xiaohui; Gao, Chunlin; Ji, Chenbo; Chen, Fukun; Zhu, Chun; Zhu, Jingai; Wang, Jialin; Qian, Lingmei; Guo, Xirong

    2010-07-01

    NYGGF4 is a recently discovered gene that is involved in obesity-associated insulin resistance. The exact mechanism by which NYGGF4 induces insulin resistance has not yet been fully elucidated. In this study, we demonstrated that the overexpression of NYGGF4 in 3T3-L1 adipocytes decreased mitochondrial mass, mitochondrial DNA, and intracellular ATP synthesis. In addition, NYGGF4 overexpression also led to an imbalance of the mitochondrial dynamics and excess intracellular ROS production. Collectively, our results indicated that the overexpression of NYGGF4 caused mitochondrial dysfunction in adipocytes, which might be responsible for the development of NYGGF4-induced insulin resistance.

  3. Measurement precision and biological variation of cranial arteries using automated analysis of 3 T magnetic resonance angiography

    DEFF Research Database (Denmark)

    Amin, Faisal Mohammad; Lundholm, Elisabet; Hougaard, Anders;

    2014-01-01

    -to-day, and side-to-side variations of the circumference of the middle meningeal (MMA) and middle cerebral (MCA) arteries. RESULTS: We found an excellent intra- and interobserver agreement for the MMA (ICC: 0.909-0.987) and for the MCA (ICC: 0.876-0.949). The coefficient of variance within observers was ≤1......-to-day and side-to-side variations have not yet been published. We hypothesised that the observer related, side-to-side, and day-to-day variations would be less than 10%. METHODS: Ten female participants were studied using high-resolution MRA on two study days separated by at least one week. Using the automated...... LKEB-MRA vessel wall analysis software arterial circumferences were measured by blinded observers. Each artery was analysed twice by each of the two different observers. The primary endpoints were to determine the intraclass correlation coefficient (ICC) and intra- an inter-observer, the day...

  4. Differentiation of hemangioblastomas from pilocytic astrocytomas using 3-T magnetic resonance perfusion-weighted imaging and MR spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    She, D.J.; Xing, Z.; Zeng, Z.; Cao, D.R. [First Affiliated Hospital of Fujian Medical University, Department of Radiology, Fuzhou, Fujian (China); Shang, X.Y. [University of California, San Diego, Department of Medicine and the Moores UCSD Cancer Center, La Jolla, CA (United States)

    2015-03-01

    Hemangioblastomas and pilocytic astrocytomas (PAs) present similar imaging features on conventional MR imaging, making differential diagnosis a challenge. The purpose of this study was to evaluate the usefulness of dynamic susceptibility-weighted contrast-enhanced perfusion-weighted imaging (DSC-PWI) and proton MR spectroscopic imaging in the differentiation of hemangioblastomas and PAs. A 3.0-T MR imaging unit was used to perform DSC-PWI and conventional MR imaging on 14 patients with hemangioblastomas and 22 patients with PAs. Four patients with hemangioblastomas and 10 PA patients also underwent proton MR spectroscopy. Parameters of relative peak height (rPH) and relative percentage of signal intensity recovery (rPSR) were acquired by DSC-PWI and variables of N-acetylaspasrtate (NAA)/creatine (Cr), choline (Cho)/Cr, and lactate-lipid (Lac-Lip)/Cr by MR spectroscopy. The sensitivity, specificity, and the area under the receiver operating characteristic curve of all analyzed parameters at respective cutoff values were determined. Higher rPH but lower rPSR values were detected in hemangioblastomas compared to PAs. The NAA/Cr ratio was significantly lower in hemangioblastomas compared with PAs. The threshold values ≥3.2 for rPH provide sensitivity, specificity, positive predictive values, and negative predictive values of 85.7, 95.5, 92.3, and 91.3 %, respectively, for differentiating hemangioblastomas from PAs. The optimal threshold values were ≤0.9 for rPSR and ≤1.5 for NAA/Cr ratios in tumor. Significantly higher rPH and lower NAA/Cr were seen in patients with hemangioblastomas when compared with PA patients, suggesting that DSC-PWI and proton MR spectroscopy are helpful in the characterization and differentiation of these two types of tumors. (orig.)

  5. Investigating the prediction value of multiparametric magnetic resonance imaging at 3 T in response to neoadjuvant chemotherapy in breast cancer

    Energy Technology Data Exchange (ETDEWEB)

    Minarikova, Lenka; Bogner, Wolfgang; Zaric, Olgica; Trattnig, Siegfried; Gruber, Stephan [Medical University of Vienna, High-field MR Centre, Department of Biomedical Imaging and Image-guided Therapy, Vienna (Austria); Christian Doppler Laboratory for Clinical Molecular MR Imaging, Vienna (Austria); Pinker, Katja [Medical University of Vienna, Division of Molecular and Gender Imaging, Department of Biomedical Imaging and Image-guided Therapy, Vienna (Austria); Memorial Sloan-Kettering Cancer Center, Molecular Imaging and Therapy Service, New York, NY (United States); Valkovic, Ladislav [Medical University of Vienna, High-field MR Centre, Department of Biomedical Imaging and Image-guided Therapy, Vienna (Austria); Christian Doppler Laboratory for Clinical Molecular MR Imaging, Vienna (Austria); Slovak Academy of Sciences, Department of Imaging Methods, Institute of Measurement Science, Bratislava (Slovakia); University of Oxford, John Radcliffe Hospital, Oxford Centre for Clinical Magnetic Resonance Research, Oxford (United Kingdom); Bago-Horvath, Zsuzsanna [Medical University of Vienna, Department of Pathology, Comprehensive Cancer Center, Vienna (Austria); Bartsch, Rupert [Medical University of Vienna, Clinical Division of Oncology, Department of Medicine I, Vienna (Austria); Helbich, Thomas H. [Medical University of Vienna, Division of Molecular and Gender Imaging, Department of Biomedical Imaging and Image-guided Therapy, Vienna (Austria)

    2017-05-15

    To explore the predictive value of parameters derived from diffusion-weighted imaging (DWI) and contrast-enhanced (CE)-MRI at different time-points during neoadjuvant chemotherapy (NACT) in breast cancer. Institutional review board approval and written, informed consent from 42 breast cancer patients were obtained. The patients were investigated before and at three different time-points during neoadjuvant chemotherapy (NACT) using tumour diameter and volume from CE-MRI and ADC values obtained from drawn 2D and segmented 3D regions of interest. Prediction of pathologic complete response (pCR) was evaluated using the area under the curve (AUC) of receiver operating characteristic analysis. There was no significant difference between pathologic complete response and non-pCR in baseline size measures (p > 0.39). Diameter change was significantly different in pCR (p < 0.02) before the mid-therapy point. The best predictor was lesion diameter change observed before mid-therapy (AUC = 0.93). Segmented volume was not able to differentiate between pCR and non-pCR at any time-point. The ADC values from 3D-ROI were not significantly different from 2D data (p = 0.06). The best AUC (0.79) for pCR prediction using DWI was median ADC measured before mid-therapy of NACT. The results of this study should be considered in NACT monitoring planning, especially in MRI protocol designing and time point selection. (orig.)

  6. Interações medicamentosas potenciais com benzodiazepínicos em prescrições médicas de pacientes hospitalizados

    Directory of Open Access Journals (Sweden)

    Amanda Martins Viel

    2014-09-01

    Full Text Available A prática de associações medicamentosas é comum em pacientes hospitalizados, Esta prática é muitas vezes necessária, principalmente em pacientes psiquiátricos, uma vez que, juntamente com as doenças neuropsiquiátricas, podem ocorrer outras comorbidades, Entretanto, esta prática pode favorecer a ocorrência de interações medicamentosas com consequente potencialização de diferentes efeitos adversos, Diante deste quadro, o presente trabalho teve como objetivo avaliar a ocorrência de potenciais interações medicamentosas com os benzodiazepínicos, prescritos aos pacientes internados na Clínica Psiquiátrica do Hospital Regional de Assis – SP, a fim de gerar informações que contribuam para a eficácia do tratamento estabelecido ao paciente, Para isso, foi realizada uma análise de 100 prescrições médicas, nas quais foram avaliadas as possibilidades de ocorrência de interações medicamentosas entre os diferentes fármacos da classe dos benzodiazepínicos administrados concomitantemente, bem como com outras classes de fármacos, Por meio deste estudo, verificou-se que das 100 prescrições médicas analisadas 93 apresentaram a possibilidade de ocorrência de interações farmacológicas entre benzodiazepínicos e com outras classes de fármacos, totalizando 356 possíveis interações, Desse total, destacam-se as associações dos benzodiazepínicos com os antipsicóticos, anti-histamínicos, antiepilépticos e antidepressivos, as quais podem potencializar a manifestação de inúmeros efeitos adversos, em destaque, a exacerbação do efeito depressor do sistema nervoso central, com repercussões que podem variar desde a manifestação clínica leve até risco de êxito letal, Neste contexto, busca-se com este trabalho contribuir para uma melhor compreensão, reconhecimento e intervenção precoce ou profilática em situações clínicas decorrentes de interações farmacológicas entre diferentes classes de fármacos com os

  7. A leitura conjunta e a interação entre adultos e crianças de 3-5 anos. Revisão de antecedentes

    Directory of Open Access Journals (Sweden)

    Nicolás Arias Velandia

    2014-09-01

    Full Text Available Este artigo de revisão reporta os resultados de primeira fase de um projeto de pesquisa, que realiza uma revisão de estudos realizados previamente em torno à leitura conjunta entre adultos e crianças de três a seis anos. Parte da ideia de que a leitura conjunta é a interação entre o adulto e a criança, onde ele primeiro lê à criança e este interpreta a linguagem emitida e lida pelo adulto, que é uma atividade própria de cenários de aprendizado alfabético inicial. A metodologia utilizada foi a análise de conteúdo de fontes antigas (de 1970 a 2002, e de artigos e reportes de pesquisa de 2002 a 2012, que abordaram o tema desde dois grandes áreas de estudo: o estudo da leitura conjunta em si próprio, e o estudo geral da interação entre crianças e adultos. Os resultados desta análise mostram uma focalização dos estudos em três áreas: fatores que incidem na interação adulto – criança, tipologias de possíveis intercâmbios comunicativos e linguísticos entre adulto e criança, e dinâmicas da interação adulto – criança e o seu uso intencional em programas de intervenção. Conclui-se que a interação adulto - criança que dá-se na leitura conjunta parece estar fortemente mediada pelo nível de desenvolvimento de enunciados linguísticos complexos na criança e pela capacidade do adulto para acomodar-se às respostas da criança em cada momento, em prol de promover o conhecimento que este desenvolva com a interação social enriquecida.

  8. Prevalência de interações medicamentosas em unidades de terapia intensiva no Brasil Prevalence of drug interactions in intensive care units in Brazil

    Directory of Open Access Journals (Sweden)

    Rhanna Emanuela Fontenele Lima de Carvalho

    2013-01-01

    Full Text Available OBJETIVO: Determinar a prevalência de interações medicamentosas em unidades de terapia intensiva e analisar a significância clínica das interações identificadas. MÉTODOS: Estudo multicêntrico, transversal e retrospectivo desenvolvido com 1124 pacientes em sete unidades de terapia intensiva (UTI de hospitais de ensino no Brasil. As informações sobre os medicamentos administrados com 24 horas e 120 horas de internação foram obtidas nas prescrições. RESULTADOS: Em 24 horas 70,6% dos pacientes apresentaram pelo menos uma interação medicamentosa. O número de interações medicamentosas detectadas em 24 horas foi 2299 e em 120 horas foi 2619. Midazolam, fentanil, fenitoína e omeprazol foram os fármacos com maior frequência de interações medicamentosas. CONCLUSÃO: Nesta amostra, interações medicamentosas moderadas e graves foram mais prevalentes. Diante desses resultados, todas as ações dos profissionais de saúde que prestam assistência ao paciente devem ser integradas visando identificar e prevenir possíveis eventos a medicamentos.OBJECTIVE: To determine the prevalence of drug interactions in intensive care units and to analyze the clinical significance of interactions identified. METHODS: A multicenter, retrospective and cross sectional study conducted with 1124 patients in the seven intensive care units of teaching hospitals in Brazil. Information on drugs administered at 24 hours and 120 hours of hospitalization was obtained from the prescriptions. RESULTS: Within 24 hours, 70.6% of patients had at least one drug interaction; the number at 24h was 2299, at 120 h it was 2619. Midazolam, fentanyl, phenytoin and omeprazole were the drugs with higher frequency of drug interactions. CONCLUSION: In this sample, moderate and severe drug interactions were more prevalent. In light of these findings, all actions of health professionals who provide care to these patients must be integrated in order to identify and prevent

  9. Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking.

    Science.gov (United States)

    Sadler, Jessica B A; Bryant, Nia J; Gould, Gwyn W

    2015-02-01

    The fusion of GLUT4-containing vesicles with the plasma membrane of adipocytes is a key facet of insulin action. This process is mediated by the formation of functional soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes between the plasma membrane t-SNARE complex and the vesicle v-SNARE or VAMP. The t-SNARE complex consists of Syntaxin4 and SNAP23, and whereas many studies identify VAMP2 as the v-SNARE, others suggest that either VAMP3 or VAMP8 may also fulfil this role. Here we characterized the levels of expression, distribution, and association of all the VAMPs expressed in 3T3-L1 adipocytes to provide the first systematic analysis of all members of this protein family for any cell type. Despite our finding that all VAMP isoforms form SDS-resistant SNARE complexes with Syntaxin4/SNAP23 in vitro, a combination of levels of expression (which vary by >30-fold), subcellular distribution, and coimmunoprecipitation analyses lead us to propose that VAMP2 is the major v-SNARE involved in GLUT4 trafficking to the surface of 3T3-L1 adipocytes.

  10. TC10 is regulated by caveolin in 3T3-L1 adipocytes.

    Directory of Open Access Journals (Sweden)

    Dave Bridges

    Full Text Available BACKGROUND: TC10 is a small GTPase found in lipid raft microdomains of adipocytes. The protein undergoes activation in response to insulin, and plays a key role in the regulation of glucose uptake by the hormone. METHODOLOGY/PRINCIPAL FINDINGS: TC10 requires high concentrations of magnesium in order to stabilize guanine nucleotide binding. Kinetic analysis of this process revealed that magnesium acutely decreased the nucleotide release and exchange rates of TC10, suggesting that the G protein may behave as a rapidly exchanging, and therefore active protein in vivo. However, in adipocytes, the activity of TC10 is not constitutive, indicating that mechanisms must exist to maintain the G protein in a low activity state in untreated cells. Thus, we searched for proteins that might bind to and stabilize TC10 in the inactive state. We found that Caveolin interacts with TC10 only when GDP-bound and stabilizes GDP binding. Moreover, knockdown of Caveolin 1 in 3T3-L1 adipocytes increased the basal activity state of TC10. CONCLUSIONS/SIGNIFICANCE: Together these data suggest that TC10 is intrinsically active in vivo, but is maintained in the inactive state by binding to Caveolin 1 in 3T3-L1 adipocytes under basal conditions, permitting its activation by insulin.

  11. Digital Image Analysis of Reactive Oxygen Species and CA2+ in Mouse 3T3 Fibroblasts

    Science.gov (United States)

    Xu, Hongzhi; Liu, Dongwu; Chen, Zhiwei

    Recently, analysis of digital images with confocal microscope has become a routine technique and indispensable tool for cell biological studies and molecular investigations. Because the light emitted from the point out-of-focus is blocked by the pinhole and can not reach the detector, thus only an image of the fluorescence from the focal plane is imaged. In present studies, we use the probes 2', 7'-dichlorof luorescein diacetate (H2DCF-DA) and Fluo-3 AM to research reactive oxygen species (ROS) and Ca2+ in mouse 3T3 fibroblasts, respectively. Our results indicate that the distribution of ROS and Ca2+ were clearly seen in mouse 3T3 fibroblasts. Moreover, we acquired and quantified the fluorescence intensity of ROS and Ca2+ with Leica Confocal Software. It was found that the quantified fluorescence intensity of ROS and Ca2+ was 123.30.26±8.99 and 125.13±12.16, respectively. Taken together, our results indicate that it is a good method to research the distribution and fluorescence intensity of ROS and Ca2+ in cultured cells with confocal microscope.

  12. What is new in Office 2007 - (IT3T/2007/2)

    CERN Document Server

    CERN. Geneva

    2007-01-01

    IT3T/2007/2 - Quoi de neuf avec Office 2007 Nous allons présenter le nouveau type de site web disponible au CERN: "Collaboration Workspaces". Avec des fonctionnalités comme les forums de discussion, les pages wiki, la création rapide de formulaires web et les "document libraries", nous montrerons comment les Collaboration Workspaces permettent de publier, partager et récupérer l'information de manière plus simple et plus rapide, et permettent de collaborer de manière efficace sur les documents. IT3T/2007/2 - What is new in Office 2007 The presentation will introduce the new features of Microsoft Office 2007 including the new Microsoft Office Fluent user interface and explain how to use the Ribbon, Contextual Tabs, Galleries, and Live Preview. No special previous knowledge is required, at the same time a basic knowledge of Office 2003 applications (Word/Excel/PowerPoint) may be a convenient starting point.

  13. Behavior of MC3T3-E1 Osteoblast Cultured on Chitosan Modified with Polyvinylpyrrolidone

    Institute of Scientific and Technical Information of China (English)

    XI Jing; GAO Yuan; KONG Lijun; GONG Yandao; ZHAO Nanming; ZHANG Xiufang

    2005-01-01

    The physical and chemical properties of four kinds of modified chitosan materials made by blending chitosan with polyvinylpyrrolidone (PVP) were investigated. All four of these modified chitosan materials were hydrophilic with water contact angles ranging from 59° to 69°. Fourier transform-infrared spectra of the modified materials showed a new band at 1288 cm-1, implying formation of a surface physical interpenetrating network structure. Enzyme linked immunosorbent assay results indicated that much less fibronectin was adsorbed on the modified materials than on only chitosan. The viability of MC3T3-E1 osteoblasts cultured on the materials was assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl- 2H-tetrazolium bromide assay. The results show that adding PVP10000 into the chitosan promotes adhesion of MC3T3-E1 osteoblasts on the modified materials, but has no effect on cell growth and proliferation; while adding PVP40000 reduces cell adhesion, growth, and proliferation. The results suggest that the increased hydrophilicity of the material surface does not always improve its biocompatibility, which will influence the selection and design of biomaterials.

  14. Berberine inhibits 3T3-L1 adipocyte differentiation through the PPARgamma pathway.

    Science.gov (United States)

    Huang, Cheng; Zhang, Yuebo; Gong, Zhenwei; Sheng, Xiaoyan; Li, Zongmeng; Zhang, Wei; Qin, Ying

    2006-09-22

    Berberine (BBR), a compound purified from Cortidis rhizoma, reduces serum cholesterol, triglycerides, and LDL-cholesterol of hypercholesterolemic patients and high fat diet fed animals, and increases hepatic LDLR mRNA and protein levels through a post-transcriptional mechanism. BBR also enhances the hypoglycemic action of insulin in diabetic animal models. Here, we show that BBR inhibits the differentiation of 3T3-L1 preadipocytes induced by DM and suppresses the mitotic clonal expansion of 3T3-L1 preadipocytes in a time- and dose-dependent manner. Gene expression analysis and Western blot analysis reveal that the BBR inhibits the mRNA and protein levels of adipogenesis related transcription factors PPARgamma and C/EBPalpha and their upstream regulator, C/EBPbeta. Reporter gene assays demonstrate that the full-length PPARgamma and alpha transcription activities are inhibited by BBR. Using real-time PCR, we have also found that the PPAR target genes that are involved in adipocyte differentiation, such as aP2, CD36, ACO, LPL, and other adipocyte markers, are suppressed by BBR. These studies suggest that BBR works on multiple molecular targets as an inhibitor of PPARgamma and alpha, and is a potential weight reducing, hypolipidemic, and hypoglycemic drug.

  15. Effects of salvia miltiorrhiza bunge (SMB) on MC3T3-E1 cells.

    Science.gov (United States)

    Ding, Y; Soma, S; Takano-Yamamoto, T; Matsumoto, S; Sakuda, M

    1995-12-01

    In previous research, we found that the Salvia Miltiorrhiza Bunge (SMB), a traditional Chinese medicinal herb, accelerates orthodontic tooth movement. In the present study, to characterize the actions of SMB on bone remodeling, we investigated the effect of SMB on DNA synthesis and alkaline phosphatase activity of murine osteoblast like cell-clone, MC3T3-E1 cells in vitro. Treatment of the cells with SMB for 72 hours caused significant increase in ALPase activity. It was found that SMB increased ALPase activity in a dose-dependent manner, and up to maximum at the concentration of 5.0 mg/ml. At that concentration, ALPase activity was about 135% greater than that of control. SMB at 5.0 mg/ml significantly stimulated ALPase activity of the cells in multilayer on day 8 or calcification stage on day 16, but inhibited it in the sparse stage on day 2 or subconfluency stage on day 4. SMB had no effect on DNA synthesis in any stage of culture. ALPase activity significantly increased at 48 hours and was up to 300% greater than that of control activity at 96 hours. These findings suggest that SMB directly stimulates ALPase activity of MC3T3-E1 cells in multilayer stage or calcification stage without any effect on proliferation. Locally administrated SMB may affect the differentiation of osteoblasts in vivo.

  16. Foxp3+ T cells inhibit antitumor immune memory modulated by mTOR inhibition.

    Science.gov (United States)

    Wang, Yanping; Sparwasser, Tim; Figlin, Robert; Kim, Hyung L

    2014-04-15

    Inhibition of mTOR signaling enhances antitumor memory lymphocytes. However, pharmacologic mTOR inhibition also enhances regulatory T-cell (Treg) activity. To counter this effect, Treg control was added to mTOR inhibition in preclinical models. Tregs were controlled with CD4-depleting antibodies because CD4 depletion has high translational potential and already has a well-established safety profile in patients. The antitumor activity of the combination therapy was CD8 dependent and controlled growth of syngeneic tumors even when an adoptive immunotherapy was not used. Lymphocytes resulting from the combination therapy could be transferred into naïve mice to inhibit aggressive growth of lung metastases. The combination therapy enhanced CD8 memory formation as determined by memory markers and functional studies of immune recall. Removal of FoxP3-expressing T lymphocytes was the mechanism underlying immunologic memory formation following CD4 depletion. This was confirmed using transgenic DEREG (depletion of regulatory T cells) mice to specifically remove Foxp3(+) T cells. It was further confirmed with reciprocal studies where stimulation of immunologic memory because of CD4 depletion was completely neutralized by adoptively transferring tumor-specific Foxp3(+) T cells. Also contributing to tumor control, Tregs that eventually recovered following CD4 depletion were less immunosuppressive. These results provide a rationale for further study of mTOR inhibition and CD4 depletion in patients. ©2014 AACR.

  17. Introduction to Collaboration Workspaces using SharePoint - (IT3T/2007/1)

    CERN Document Server

    CERN. Geneva

    2007-01-01

    IT3T/2007/1: Introduction aux 'Collaboration Workspace' basés sur SharePoint Nous allons présenter le nouveau type de site web disponible au CERN: "Collaboration Workspaces". Avec des fonctionnalités comme les forums de discussion, les pages wiki, la création rapide de formulaires web et les "document libraries", nous montrerons comment les Collaboration Workspaces permettent de publier, partager et récupérer l'information de manière plus simple et plus rapide, et permettent de collaborer de manière efficace sur les documents. IT3T/2007/1: Introduction to Collaboration Workspaces using SharePoint We will introduce the new "collaboration workspace" type of web site available at CERN. With features such as discussion forums, wiki pages, quick forms and document libraries, we will show how Collaboration Workspaces can be used to make it faster and easier to publish, request and share information, and allow to efficiently collaborate on documents.

  18. Introduction to Collaboration Workspaces using SharePoint - (IT3T/2007/1)

    CERN Document Server

    CERN. Geneva

    2007-01-01

    IT3T/2007/1 - Introduction aux "Collaboration Workspace" basés sur SharePoint Nous allons présenter le nouveau type de site web disponible au CERN: "Collaboration Workspaces". Avec des fonctionnalités comme les forums de discussion, les pages wiki, la création rapide de formulaires web et les "document libraries", nous montrerons comment les Collaboration Workspaces permettent de publier, partager et récupérer l'information de manière plus simple et plus rapide, et permettent de collaborer de manière efficace sur les documents. IT3T/2007/1 - Introduction to Collaboration Workspaces using SharePoint We will introduce the new "collaboration workspace" type of web site available at CERN. With features such as discussion forums, wiki pages, quick forms and document libraries, we will show how Collaboration Workspaces can be used to make it faster and easier to publish, request and share information, and allow to efficiently collaborate on documents.

  19. Effect of pycnogenol on glucose transport in mature 3T3-L1 adipocytes.

    Science.gov (United States)

    Lee, Hee-Hyun; Kim, Kui-Jin; Lee, Ok-Hwan; Lee, Boo-Yong

    2010-08-01

    Pycnogenol, a procyanidins-enriched extract of Pinus maritima bark, possesses antidiabetic properties, which improves the altered parameters of glucose metabolism that are associated with type 2 diabetes mellitus (T2DM). Since the insulin-stimulated antidiabetic activities of natural bioactive compounds are mediated by GLUT4 via the phosphatidylinositol-3-kinase (PI3K) and/or p38 mitogen activated protein kinase (p38-MAPK) pathway, the effects of pycnogenol were examined on the molecular mechanism of glucose uptake by the glucose transport system. 3T3-L1 adipocytes were treated with various concentrations of pycnogenol, and glucose uptake was examined using a non-radioisotope enzymatic assay and by molecular events associated with the glucose transport system using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results show that pycnogenol increased glucose uptake in fully differentiated 3T3-L1 adipocytes and increased the relative abundance of both GLUT4 and Akt mRNAs through the PI3K pathway in a dose dependent manner. Furthermore, pycnogenol restored the PI3K antagonist-induced inhibition of glucose uptake in the presence of wartmannin, an inhibitor of the PI3K. Overall, these results indicate that pycnogenol may stimulate glucose uptake via the PI3K dependent tyrosine kinase pathways involving Akt. Further the results suggest that pycnogenol might be useful in maintaining blood glucose control.

  20. Fipronil promotes adipogenesis via AMPKα-mediated pathway in 3T3-L1 adipocytes.

    Science.gov (United States)

    Sun, Quancai; Qi, Weipeng; Yang, Jeremy J; Yoon, Kyong Sup; Clark, John M; Park, Yeonhwa

    2016-06-01

    Emerging evidence suggests that organochlorine, organophosphorus and neonicotinoid insecticide exposure may be linked to the development of obesity and type 2 diabetes. However, there is no knowledge of the potential influence of fipronil, which belongs to the phenylpyrazole chemical family, on obesity. Thus, the goal of this study was to determine the role of fipronil in adipogenesis using 3T3-L1 adipocytes. Fipronil treatment, at 10 μM, increased fat accumulation in 3T3-L1 adipocytes as well as promoted key regulators of adipocyte differentiation (CCAAT/enhancer-binding protein α and peroxisome proliferator-activated receptor gamma-γ), and key regulators of lipogenesis (acetyl-CoA carboxylase and fatty acid synthase). The activation of AMPKα with 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) abolished effects of fipronil on increased adipogenesis. These results suggest that fipronil alters adipogenesis and results in increased lipid accumulation through a AMPKα-mediated pathway. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Wogonin enhances intracellular adiponectin levels and suppresses adiponectin secretion in 3T3-L1 adipocytes.

    Science.gov (United States)

    Yang, Tan; Liu, Hua; Zhao, Bo; Xia, Zhongyuan; Zhang, Yemin; Zhang, Deling; Li, Mingxin; Cao, Yingkang; Zhang, Zhijiang; Bi, Yongyi; Wang, Changhua

    2017-01-30

    As an insulin sensitizer and modulator of inflammatory responses, adiponectin has become a therapeutic target for insulin resistance, diabetes, and diabetes-related complications. Wogonin possesses anti-oxidative, anti-inflammatory, and anti-diabetic abilities. However, its effect on generation and secretion of adiponectin is ill-defined in adipocytes. Here, we demonstrated that wogonin administration augmented intracellular adiponectin levels and attenuated adiponectin release in a dose- and time-dependent manner in mature 3T3-L1 adipocytes, along with a suppression of PKCδ phosphorylation. Wogonin treatment also prevented PKCδ overexpression-induced reduction of intracellular adiponectin levels and enhancement of adiponectin release. In addition, wogonin supplementation dramatically increased AMPK phosphorylation and SirT1 expression. Inhibition of either AMPK or SirT1 mitigated wogonin action on adiponectin production and release. Furthermore, inhibition of AMPK by its specific inhibitor markedly reduced wogonin-enhanced mRNA and protein expressions of SirT1. These results suggested that wogonin regulated expression and secretion of adiponectin via PKCδ/AMPK/SirT1 signaling pathway in mature 3T3-L1 adipocytes.

  2. Combination therapy with catechins and caffeine inhibits fat accumulation in 3T3-L1 cells

    Science.gov (United States)

    Zhu, Xiaojuan; Yang, Licong; Xu, Feng; Lin, Lezhen; Zheng, Guodong

    2017-01-01

    Catechins and caffeine, which are green tea components, have a slimming effect; however, the combinational effect of fat metabolism in 3T3-L1 cells remains unclear. In the present study, 3T3-L1 cells were treated with catechins and caffeine in combination, and it was found that combination therapy with catechins and caffeine markedly reduced intracellular fat accumulation, mRNA expression levels of peroxisome proliferator-activated receptor-γ and CCAAT/enhancer-binding protein α in the early stage of cell differentiation were significantly reduced, and mRNA expression of fatty acid synthetase(FAS) andglycerol-3-phosphate dehydrogenase protein expression levels of FAS were downregulated. Noradrenaline-induced lipolysis was enhanced by caffeine, which markedly increased the protein expression of adipose triglyceride lipase and hormone sensitive lipase. These results indicated that combination therapy with catechins and caffeine synergistically inhibited lipid accumulation by regulating the gene and protein expression levels of lipid metabolism-related enzymes. Therefore, catechins and caffeine combination therapy has potential as a functional food that may be used to prevent obesity and lifestyle-associated diseases. PMID:28352352

  3. Protein kinase A suppresses the differentiation of 3T3-L1 preadipocytes

    Institute of Scientific and Technical Information of China (English)

    Fuqiang Li; Dongmei Wang; Yiran Zhou; Bo Zhou; Yanan Yang; Hehua Chen; Jianguo Song

    2008-01-01

    cAMP and protein kinase A (PKA) are widely known as signaling molecules that are important for the induction of adipogenesis. Here we show that a strong increase in the amount of cAMP inhibits the adipogenesis of 3T3-L1 fibroblast cells. Stimulation of PKA activity suppresses adipogenesis and, in contrast, inhibition of PKA activity markedly accelerates the adipogenic process. As adipogenesis progresses, there is a significant increase in the expression level of PKA regulatory subunits and a corresponding decrease in PKA activity. Moreover, treatment of 3T3-L1 cells with epidermal growth factor (EGF) stimulates PKA activity and blocks adipogenesis. Inhibition of PKA activity abolishes this suppressive effect of EGF on adipogenesis. Moreover, activation of PKA induces serine/threonine phosphorylation, reduces tyrosine phosphorylation of insulin receptor substrate 1 (IRS-1) and the association between PKA and IRS-1. Taken together, our study demonstrates that PKA has a pivotal role in the suppression of adipogenesis. cAMP at high concentrations can suppress adipogenesis through PKA activation. These findings could be important and useful for understanding the mechanisms of adipogenesis and the relevant physiological events.

  4. Anti-adipogenic effect of mulberry leaf ethanol extract in 3T3-L1 adipocytes.

    Science.gov (United States)

    Yang, Soo Jin; Park, Na-Young; Lim, Yunsook

    2014-12-01

    Adipogenesis is part of the cell differentiation process in which undifferentiated fibroblasts (pre-adipocytes) become mature adipocytes with the accumulation of lipid droplets and subsequent cell morphological changes. Several transcription factors and food components have been suggested to be involved in adipogenesis. The aim of this study was to determine whether mulberry leaf ethanol extract (MLEE) affects adipogenesis in 3T3-L1 adipocytes. The 3T3-L1 adipocytes were treated with different doses of MLEE for 8 days starting 2 days post-confluence. Cell viability, fat accumulation, and adipogenesis-related factors including CCAAT-enhancer-binding protein alpha (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ), PPARγ coactivator 1 alpha (PGC-1α), fatty acid synthase (FAS), and adiponectin were analyzed. Results showed that MLEE treatments at 10, 25, 50, and 100 µg/ml had no effect on cell morphology and viability. Without evident toxicity, all MLEE treated cells had lower fat accumulation compared with control as shown by lower absorbances of Oil Red O stain. MLEE at 50 and 100 µg/ml significantly reduced protein levels of PPARγ, PGC-1α, FAS, and adiponectin in differentiated adipocytes. Furthermore, protein level of C/EBPα was significantly decreased by the treatment of 100 µg/ml MLEE. These results demonstrate that MLEE treatment has an anti-adipogenic effect in differentiated adipocytes without toxicity, suggesting its potential as an anti-obesity therapeutic.

  5. Oxidative changes and apoptosis induced by 1800-MHz electromagnetic radiation in NIH/3T3 cells.

    Science.gov (United States)

    Hou, Qingxia; Wang, Minglian; Wu, Shuicai; Ma, Xuemei; An, Guangzhou; Liu, Huan; Xie, Fei

    2015-03-01

    To investigate the potential adverse effects of mobile phone radiation, we studied reactive oxygen species (ROS), DNA damage and apoptosis in mouse embryonic fibroblasts (NIH/3T3) after intermittent exposure (5 min on/10 min off, for various durations from 0.5 to 8 h) to an 1800-MHz GSM-talk mode electromagnetic radiation (EMR) at an average specific absorption rate of 2 W/kg. A 2',7'-dichlorofluorescin diacetate fluorescence probe was used to detect intracellular ROS levels, immunofluorescence was used to detect γH2AX foci as a marker for DNA damage, and flow cytometry was used to measure apoptosis. Our results showed a significant increase in intracellular ROS levels after EMR exposure and it reached the highest level at an exposure time of 1 h (p < 0.05) followed by a slight decrease when the exposure continued for as long as 8 h. No significant effect on the number of γH2AX was detected after EMR exposure. The percentage of late-apoptotic cells in the EMR-exposed group was significantly higher than that in the sham-exposed groups (p < 0.05). These results indicate that an 1800-MHz EMR enhances ROS formation and promotes apoptosis in NIH/3T3 cells.

  6. Feasibility of 3-T MRI for the evaluation of Crohn disease in children

    Energy Technology Data Exchange (ETDEWEB)

    Dagia, Charuta; Ditchfield, Michael; Kean, Michael [Royal Children' s Hospital, Department of Medical Imaging and Murdoch Childrens Research Institute, Victoria (Australia); Catto-Smith, Anthony [Royal Children' s Hospital, Department of Gastroenterology, Victoria (Australia)

    2010-10-15

    Crohn disease (CD) is a chronic inflammatory bowel disease that can affect any part of the gastrointestinal tract from the oral cavity to the anal canal. It occurs in all ages and is a significant cause for morbidity in children. Interest in MRI evaluation of CD has increased because of the concern regarding cumulative radiation dose from contrast fluoroscopic studies and CT. Several reports have demonstrated MRI to be a useful technique for CD. Most of these studies were performed at 1.5-T field strength. Imaging at a higher field strength, with a greater signal-to-noise ratio, has the potential of reducing scan times and increasing the resolution. However, there is a concurrent increase in artefacts, and these can be pronounced with abdominal imaging at 3 T. To determine the feasibility of 3-T MRI for CD in children and to assess the value of different sequences and the effect of artefacts that could potentially limit the role of bowel MR imaging at higher field strengths. A retrospective study of 46 children with biopsy-proven CD (ages 8-19 years, 53% boys) was performed. Sixty-eight consecutive MRI studies were performed on a 3-T scanner between 2005 and 2007; 42 of the abdomen (62%) and 26 of the pelvis/perineum (38%). Sorbitol was administered for the abdominal studies; orally for 36/42 (86%) studies and via a naso-jejunal (NJ) tube for 6/42 (14%) studies. For the abdomen, T2-W half-fourier acquisition single-shot turbo spin-echo (T2-W HASTE), true steady-state free precession (true FISP), pre-contrast and contrast-enhanced (CE) T1-volume interpolated gradient-echo (T1-W VIBE) and CE T1-W fast low-angle shot (T1-W FLASH) sequences were performed. For the perianal and pelvic assessment, fat-saturated T2-W turbo spin-echo (TSE), pre-contrast and CE T1-W FLASH or VIBE sequences were performed. The sequences were scored for diagnostic quality by two paediatric radiologists for visualisation of the bowel wall, whether normal or pathological and the visualization

  7. Interação entre Hb B2 e Hb S Interaction between Hb B2 and Hb S

    Directory of Open Access Journals (Sweden)

    Natália Ferreira

    2010-02-01

    Full Text Available As hemoglobinopatias e talassemias constituem as afecções genéticas mais comuns, apresentando-se, na maioria dos casos, em heterozigose. Diante da diversidade de hemoglobinas variantes encontrada na população brasileira, metodologias específicas e complementares para um diagnóstico laboratorial preciso, capaz de elucidar possíveis interações entre estas variantes genéticas, são necessárias. Este relato de caso descreve a interação entre hemoglobina B2 e a hemoglobina S em um indivíduo do sexo feminino, caucasoide, proveniente da região Sudeste do Brasil, identificada por meio de técnicas eletroforéticas em diferentes pH, cromatografia líquida de alta performance e PCR- RFLP. Visto que a hemoglobina B2 coelui com a hemoglobina S na análise cromatográfica e dificilmente é visualizada em eletroforese pH alcalino, devido à sua baixa concentração, justifica-se a necessidade da associação de testes laboratoriais, inclusive moleculares, na rotina do diagnóstico de hemoglobinas para a correta identificação do perfil de hemoglobinas do indivíduo e real frequência na população brasileira. Rev. Bras. Hematol. Hemoter.Hemoglobinopathies and thalassemias are the most common genetic diseases, and in most cases, present as heterozygous. Due to the diversity of hemoglobin variants, specific and complementary methodologies are necessary for a precise laboratorial diagnosis, able to elucidate possible interactions between genetic polymorphisms. This case report describes an interaction between hemoglobin B2 and hemoglobin S in a Caucasian woman from the southeastern region of Brazil. This interaction was identified by electrophoresis in different pHs, high performance liquid chromatography and PCR-RFLP. As hemoglobin B2 is eluted in the same window as hemoglobin S in automatic HPLC systems and is hardly seen in alkaline electrophoresis due to its low concentration, the association must be confirmed using additional laboratorial

  8. Synthesis, crystal structure and physical properties of EuTGe{sub 3} (T = Co, Ni, Rh, Pd, Ir, Pt) single crystals

    Energy Technology Data Exchange (ETDEWEB)

    Bednarchuk, Oleksandr; Gągor, Anna; Kaczorowski, Dariusz, E-mail: D.Kaczorowski@int.pan.wroc.pl

    2015-02-15

    Highlights: • Single crystals of EuTGe{sub 3} (T = Co, Ni, Rh, Pd, Ir, Pt) compounds were prepared. • The crystal structures were refined from single-crystal X-ray diffraction data. • The physical properties of oriented single crystals were determined. • For each compound, an antiferromagnetic ordering was found at low temperatures. - Abstract: Single crystals of six EuTGe{sub 3} compounds with T = Co, Ni, Rh, Pd, Ir and Pt were prepared by high-temperature solution growth method. Their crystal structures of the BaNiSn{sub 3}-type were refined from single-crystal X-ray diffraction data. The physical properties of oriented single-crystalline specimens were studied by means of magnetic susceptibility, heat capacity and electrical resistivity measurements. For each compound, an antiferromagnetic ordering was found to set in at low temperatures due to localized magnetic moments carried on divalent Eu ions. Despite the same crystal structure, the EuTGe{sub 3} compounds exhibit diverse magnetic structures with different directions of the ordered magnetic moments and a variety of dissimilar mutual arrangements of adjacent spins.

  9. 利培酮抑制3T3-L1前脂肪细胞的分化%Risperidone inhibits 3T3-L1 pre-adipocytes differentiation

    Institute of Scientific and Technical Information of China (English)

    张高丽; 张弋; 于海川; 张新雅

    2016-01-01

    Objective To investigate the influence of risperidone on differentiation of 3T3‐L1 pre‐adipocytes .Methods 3T3‐L1 pre‐adipocytes were induced to differentiate into mature adipocytes by adopting the classic hormone cocktail method and observed by the oil red O staining .Meanwhile ,the inducing medium was added with risperidone for studying its influence on 3T3‐L1 pre‐adi‐pocytes differentiation .Results 3T3‐L1 pre‐adipocytes were successfully differentiated into the mature adipocytes ,0 .1 ,1 ,10μmol/L risperidone all could inhibit the differentiation of 3T3‐L1 pre‐adipocytes .Conclusion Risperidone can inhibit the differentiation of 3T3‐L1 pre‐adipocytes .%目的:研究利培酮对3T3‐L1前脂肪细胞分化的影响。方法采用经典的激素鸡尾酒法诱导3T3‐L1前脂肪细胞分化为成熟的脂肪细胞,油红O染色观察。向诱导培养基中加入利培酮研究其对3T3‐L1前脂肪细胞分化的影响。结果用激素鸡尾酒法成功地将3T3‐L1前脂肪细胞诱导为成熟的脂肪细胞。0.1、1、10μmol/L的利培酮均能够抑制3T3‐L1前脂肪细胞的分化。结论利培酮能够抑制3T3‐L1前脂肪细胞的分化。

  10. The role of DTI in early detection of cervical spondylotic myelopathy: a preliminary study with 3-T MRI

    Energy Technology Data Exchange (ETDEWEB)

    Kara, Batuhan [Bakirkoey Dr. Sadi Konuk Teaching Hospital, Department of Radiology, Istanbul (Turkey); Celik, Azim [General Electric Healthcare, Istanbul (Turkey); Karadereler, Selhan [Florence Nightingale Hospital, Department of Neurosurgery, Istanbul (Turkey); Ulusoy, Levent; Ganiyusufoglu, Kursat; Onat, Levent; Mutlu, Ayhan; Sirvanci, Mustafa [Florence Nightingale Hospital, Department of Radiology, Istanbul (Turkey); Ornek, Ibrahim [Florence Nightingale Hospital, Department of Neurology, Istanbul (Turkey); Hamzaoglu, Azmi [Florence Nightingale Hospital, Department of Orthopedic Surgery, Istanbul (Turkey)

    2011-08-15

    The radiological diagnosis of cervical spondylotic myelopathy (CSM) has to be made as soon as possible, since surgery performed in earlier stages during the course of CSM was reported to be more successful when compared with later stages. We hypothesized that diffusion tensor imaging (DTI) may detect CSM in earlier stages, before the appearance of signal increase in T2-weighted sequences. A total of 16 patients with neurological signs and symptoms of CSM but without hyperintensity in spinal cord on T2-weighted sequences enrolled in the study. The magnetic resonance (MR) examinations were performed on a 3-T MR imaging system. Apparent diffusion coefficient (ADC) and fractional anisotropy (FA) maps were generated on axial plane. The ADC and FA measurements in each individual were made at the level of most severe cervical canal stenosis and at a nonstenotic level. Student's t test was used to compare FA and ADC values of the spinal cord in stenotic and nonstenotic segments. We also investigated if there was a correlation between DTI parametrics and duration of clinical symptoms by using Pearson correlation analysis. All patients showed changes in DTI parametrics at stenotic segments. While FA values of the spinal cord at the stenotic level showed a statistically significant reduction, there was a statistically significant increase in the measured ADC values (p < 0.001). There was no statistical correlation between the duration of symptoms and DTI parametrics. Our preliminary findings indicate that DTI may show abnormalities in the spinal cord before the development of T2 hyperintensity on conventional sequences in patients with CSM. (orig.)

  11. Comparing the microvascular specificity of the 3 T and 7 T BOLD response using ICA and Susceptibility-Weighted Imaging

    Directory of Open Access Journals (Sweden)

    Alexander eGeissler

    2013-08-01

    Full Text Available In functional MRI it is desirable for the blood-oxygenation level dependent (BOLD signal to be localized to the tissue containing activated neurons rather than the veins draining that tissue. This study addresses the dependence of the specificity of the BOLD signal – the relative contribution of the BOLD signal arising from tissue compared to venous vessels – on magnetic field strength. To date, studies of specificity have been based on models or indirect measures of BOLD sensitivity such as signal to noise ratio and relaxation rates, and assessment has been made in isolated vein and tissue voxels. The consensus has been that ultra high field systems not only significantly increase BOLD sensitivity but also specificity, that is, there is a proportionately reduced signal contribution from draining veins. Specificity was not quantified in prior studies, however, due to the difficulty of establishing a reliable network of veins in the activated volume. In this study we use a map of venous vessel networks extracted from 7 T high resolution Susceptibility Weighted Images (SWI to quantify the relative contributions of micro- and macrovasculature to functional MRI (fMRI results obtained at 3 T and 7 T. High resolution measurements made here minimize the contribution of physiological noise and Independent Component Analysis (ICA is used to separate activation from technical, physiological and motion artifacts. ICA also avoids the possibility of timing-dependent bias from different micro- and macrovasculature responses. We find a significant increase in the number of activated voxels at 7 T in both the veins and the microvasculature – a BOLD sensitivity increase - with the increase in the microvasculature being higher. However, the small increase in sensitivity at 7 T was not significant. For the experimental conditions of this study, our findings do not support the hypothesis of an increased specificity of the BOLD response at ultra-high field.

  12. Correlation of histological findings from a large ciliochoroidal melanoma with CT perfusion and 3T MRI dynamic enhancement studies

    Directory of Open Access Journals (Sweden)

    Jose S Pulido

    2008-06-01

    Full Text Available Jose S Pulido1, Norbert G Campeau2, Ernst Klotz3, Andrew N Primak2, Osama Saba3, Kaan Gunduz1, Herbert Cantrill5, Diva Salomão1,4, Cynthia H McCollough21Department of Ophthalmology; 2Department of Radiology; 3Siemens Medical Solutions, Malvern, PA, USA; 4Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA; 5VitreoRetinal Surgery, Minneapolis, MN, USABackground: The initial use of a 64-slice computed tomography (CT scanner for obtaining quantitative perfusion data from a large ciliochoroidal melanoma, and correlation with 3T magnetic resonance imaging (MRI dynamic enhancement and tumor histology.Methods: The CT perfusion scan was performed using 80 kVp, 250 mA and 1-sec rotation time for 40 sec. The analysis was performed using commercial perfusion analysis software with a prototype 3-dimensional motion correction tool. Dynamic contrast-enhanced 3-Tesla MRI measured the kinetics of enhancement to estimate the vascular permeability. The time-dependent enhancement patterns were obtained using the average signal intensity using Functool analysis software. The involved globe was enucleated and microscopic evaluation of the tumor was performed.Results: The perfusion parameters blood flow, blood volume and permeability surface area product in the affected eye determined by CT perfusion analysis were 118 ml/100 ml/min, 11.3 ml/100 ml and 48 ml/100 ml/min. Dynamic MRI enhancement showed maximal intensity increase of 111%. The neoplasm was a ciliochoroidal spindle cell melanoma which was mitotically active (13 mitoses/40 hpf. Vascular loops and arcades were present throughout the tumor. The patient developed metastases within 9 months of presentation.Conclusion: Quantitative CT perfusion analysis of ocular tumors is feasible with motion correction software.Keywords: ciliochoroidal melanoma, CT perfusion imaging, MR enhancement imaging, tumor blood volume, tumor blood flow, tumor permeability

  13. O papel da atenção farmacêutica frente às interações fármaco-nutriente

    OpenAIRE

    Aline de Oliveira ANTUNES; Ana Cristina LO PRETE

    2014-01-01

    O farmacêutico, por meio da atenção farmacêutica, pode acompanhar a farmacoterapia, orientando e intervindo quando necessário, buscando melhoria na educação em saúde e na qualidade de vida do paciente. O objetivo deste trabalho foi realizar uma revisão bibliográfica sobre a importância da atenção farmacêutica na identificação e resolução das interações fármaco-nutriente, evidenciando as implicações dessas interações no efeito terapêutico do fármaco ou no estado nutricional. Foram ...

  14. Evaluation of chylomicron effect on ASP production in 3T3-L1 adipocytes

    Institute of Scientific and Technical Information of China (English)

    Ying Gao; Danny Gauvreau; Wei Cui; Marc Lapointe; Sabina Paglialunga; Katherine Cianflone

    2011-01-01

    In the past few years,there has been increasing interest in the production and physiological role of acylation-stimu-lating protein(ASP),identical to C3adesArg,a product of the alternative complement pathway generated through C3 cleavage.Recent studies in C3(-/-)mice that are ASP deficient have demonstrated a role for ASP in postprandial triglyceride clearance and fat storage.The aim of the present study was to establish a cell model and sensitive ELISA assay for the evaluation of ASP production using 3T3-L1 adipocytes.3T3-L1 preadipocytes were differentiated into adipocytes,then cultured in different media such as serum-free(SF),Dulbecco's modified Eagle's medium(DMEM)/F12+10% fetal calf serum (FBS),and at varying concentrations of chylomicrons and insulin+chylomicrons up to 48 h.ASP production in SF and DMEM/F12+10% FBS was compared.Chylomicrons stimulated ASP production in a concen-tration- and time-dependent manner.By contrast,chylo-micron treatment had no effect on the production of C3,the precursor protein of ASP,which was constant over 48 h.Addition of insulin(100 nM)to a low-dose of chylomicrons(100 μg TG/ml)significantly increased ASP production compared with chylomicrons alone at 48 h(P<0.001).Furthermore,addition of insulin significantly increased C3 secretion at both 18 and 48 h of incubation (P<0.05,P<0.001,respectively).Overall,the proportion of ASP to C3 remained constant,indicating no change in the ratio of C3 cleaved to generate ASP.This study demonstrated that 3T3-L1 adipocyte is a useful model for the evaluation of C3 secretion and ASP production by using a sensitive mouse-specific ELISA assay.The stimulation of ASP production with chylomicrons demonstrates a physiologically relevant response,and provides a strategy for further studies on ASP production and function.

  15. Resveratrol potentiates genistein's antiadipogenic and proapoptotic effects in 3T3-L1 adipocytes.

    Science.gov (United States)

    Rayalam, Srujana; Della-Fera, Mary Anne; Yang, Jeong-Yeh; Park, Hea Jin; Ambati, Suresh; Baile, Clifton A

    2007-12-01

    Genistein (G) and resveratrol (R) individually inhibit adipogenesis in 3T3-L1 adipocytes and induce apoptosis in cancer cells. We investigated whether the combination of G and R resulted in enhanced effects on adipogenesis, lipolysis, and apoptosis in 3T3-L1 cells. Preadipocytes and mature adipocytes were treated with G and R individually at 50 and 100 micromol/L (G100; R100) and in combination. Both in preadipocytes and mature adipocytes, G and R individually decreased cell viability dose-dependently, but G100 + R100 further decreased viability by 59 +/- 0.97% (P < 0.001) and 69.7 +/- 1.2% (P < 0.001) after 48 h compared with G100 and R100, respectively. G100 + R100 induced apoptosis 242 +/- 8.7% (P < 0.001) more than the control after 48 h, whereas G100 and R100 individually increased apoptosis only 46 +/- 9.2 and 46 +/- 7.9%, respectively. G and R did not modulate mitogen-activated protein kinase expression by themselves, but G100 + R100 increased Jun-N-terminal kinase phosphorylation by 38.8 +/- 4.4% (P < 0.001) and decreased extracellular signal-regulating kinase phosphorylation by 48 +/- 3.4% (P < 0.001). Individually, G and R at 25 micromol/L (G25; R25) decreased lipid accumulation by 30 +/- 1.7% and 20.07 +/- 4.27%, respectively (P < 0.001). However, G25 + R25 decreased lipid accumulation by 77.9 +/- 3.4% (P < 0.001). Lipolysis assay revealed that neither G25 nor R25 induced lipolysis, whereas G25 + R25 significantly increased lipolysis by 25.5 +/- 4.6%. The adipocyte-specific proteins PPARgamma and CCAAT/enhancer binding protein-alpha were downregulated after treatment with G + R, but no effect was observed with individual compounds. These results indicate that G and R in combination produce enhanced effects on inhibiting adipogenesis, inducing apoptosis, and promoting lipolysis in 3T3-L1 adipocytes. Thus, the combination of G and R is more potent in exerting antiobesity effects than the individual compounds.

  16. Ursolic acid inhibits adipogenesis in 3T3-L1 adipocytes through LKB1/AMPK pathway.

    Directory of Open Access Journals (Sweden)

    Yonghan He

    Full Text Available BACKGROUND: Ursolic acid (UA is a triterpenoid compound with multiple biological functions. This compound has recently been reported to possess an anti-obesity effect; however, the mechanisms are less understood. OBJECTIVE: As adipogenesis plays a critical role in obesity, the present study was conducted to investigate the effect of UA on adipogenesis and mechanisms of action in 3T3-L1 preadipocytes. METHODS AND RESULTS: The 3T3-L1 preadipocytes were induced to differentiate in the presence or absence of UA for 6 days. The cells were determined for proliferation, differentiation, fat accumulation as well as the protein expressions of molecular targets that regulate or are involved in fatty acid synthesis and oxidation. The results demonstrated that ursolic acid at concentrations ranging from 2.5 µM to 10 µM dose-dependently attenuated adipogenesis, accompanied by reduced protein expression of CCAAT element binding protein β (C/EBPβ, peroxisome proliferator-activated receptor γ (PPARγ, CCAAT element binding protein α (C/EBPα and sterol regulatory element binding protein 1c (SREBP-1c, respectively. Ursolic acid increased the phosphorylation of acetyl-CoA carboxylase (ACC and protein expression of carnitine palmitoyltransferase 1 (CPT1, but decreased protein expression of fatty acid synthase (FAS and fatty acid-binding protein 4 (FABP4. Ursolic acid increased the phosphorylation of AMP-activated protein kinase (AMPK and protein expression of (silent mating type information regulation 2, homolog 1 (Sirt1. Further studies demonstrated that the anti-adipogenic effect of UA was reversed by the AMPK siRNA, but not by the Sirt1 inhibitor nicotinamide. Liver kinase B1 (LKB1, the upstream kinase of AMPK, was upregulated by UA. When LKB1 was silenced with siRNA or the inhibitor radicicol, the effect of UA on AMPK activation was diminished. CONCLUSIONS: Ursolic acid inhibited 3T3-L1 preadipocyte differentiation and adipogenesis through the LKB1/AMPK

  17. The pol3-t Hyperrecombination Phenotype and DNA Damage-Induced Recombination in Saccharomyces cerevisiae Is RAD50 Dependent

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    Alvaro Galli

    2009-01-01

    Full Text Available The DNA polymerase δ (POL3/CDC2 allele pol3-t of Saccharomyces cerevisiae has previously been shown to be sensitive to methylmethanesulfonate (MMS and has been proposed to be involved in base excision repair. Our results, however, show that the pol3-t mutation is synergistic for MMS sensitivity with MAG1, a known base excision repair gene, but it is epistatic with rad50Δ, suggesting that POL3 may be involved not only in base excision repair but also in a RAD50 dependent function. We further studied the interaction of pol3-t with rad50Δ by examining their effect on spontaneous, MMS-, UV-, and ionizing radiation-induced intrachromosomal recombination. We found that rad50Δ completely abolishes the elevated spontaneous frequency of intrachromosomal recombination in the pol3-t mutant and significantly decreases UV- and MMS-induced recombination in both POL3 and pol3-t strains. Interestingly, rad50Δ had no effect on γ-ray-induced recombination in both backgrounds between 0 and 50 Gy. Finally, the deletion of RAD50 had no effect on the elevated frequency of homologous integration conferred by the pol3-t mutation. RAD50 is possibly involved in resolution of replication forks that are stalled by mutagen-induced external DNA damage, or internal DNA damage produced by growing the pol3-t mutant at the restrictive temperature.

  18. Stabilization of Nrf2 protein by D3T provides protection against ethanol-induced apoptosis in PC12 cells.

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    Jian Dong

    Full Text Available Previous studies have demonstrated that maternal ethanol exposure induces a moderate increase in Nrf2 protein expression in mouse embryos. Pretreatment with the Nrf2 inducer, 3H-1, 2-dithiole-3-thione (D3T, significantly increases the Nrf2 protein levels and prevents apoptosis in ethanol-exposed embryos. The present study, using PC12 cells, was designed to determine whether increased Nrf2 stability is a mechanism by which D3T enhances Nrf2 activation and subsequent antioxidant protection. Ethanol and D3T treatment resulted in a significant accumulation of Nrf2 protein in PC 12 cells. CHX chase analysis has shown that ethanol treatment delayed the degradation of Nrf2 protein in PC12 cells. A significantly greater decrease in Nrf2 protein degradation was observed in the cells treated with D3T alone or with both ethanol and D3T. In addition, D3T treatment significantly reduced ethanol-induced apoptosis. These results demonstrate that the stabilization of Nrf2 protein by D3T confers protection against ethanol-induced apoptosis.

  19. A interação retórico-discursiva e suas múltiplas variáveis

    Directory of Open Access Journals (Sweden)

    Melliandro Mendes Galinari

    2012-11-01

    Full Text Available O presente artigo procura construir uma visão de conjunto acercados elementos envolvidos numa interação retórico-discursiva e suasrespectivas designações teórico-conceituais. A enunciação, vista sobuma ótica argumentativa e entendida como um processo comunicativoestabelecido entre uma instância de produção e outra de recepção dodiscurso, institui-se pela atuação de inúmeras variáveis. Comoexemplo, pode-se citar os próprios argumentos veiculados pelodiscurso (logos, ethos e pathos, as modalidades possíveis de adesão(teses, ações e emoções, o gênero discursivo/textual e uma série defatores situacionais (orador, auditório, elementos dóxicos,características sócio-históricas, etc.. O artigo, enfim, salienta aimportância de se levar em consideração, conjuntamente, taiselementos durante uma análise discursiva e procura aproximar aAnálise do Discurso da Retórica e da Sofística.

  20. Diagnóstico de HPV: o processo de interação da mulher com seu parceiro

    Directory of Open Access Journals (Sweden)

    Octavio Muniz da Costa Vargens

    2013-06-01

    Full Text Available Pesquisa descritiva, com abordagem qualitativa, que objetivou analisar o processo de interação da mulher com seu parceiro a partir do diagnóstico de infecção pelo papilomavírus humano (HPV. Foi realizada em treze comunidades dos municípios de Duque de Caxias e Nova Iguaçu, Estado do Rio de Janeiro, de outubro/2006 a setembro/2008. Foram entrevistadas 20 mulheres com diagnóstico de infecção pelo HPV de alto risco oncogênico. Os pressupostos do Interacionismo Simbólico e da Grounded Theory nortearam a coleta e análise dos dados. Os resultados revelaram que o diagnóstico do HPV significa sérios desafios no relacionamento das mulheres com seu parceiro principalmente no que se refere à adoção de medidas preventivas. Conclui-se que estas questões indicam a necessidade de um atendimento humanizado que favoreça o empoderamento das mulheres.

  1. Conflito de interação instrumental e falência cognitiva no trabalho bancário informatizado

    Directory of Open Access Journals (Sweden)

    Mário César Ferreira

    1997-12-01

    Full Text Available Buscando superar o clássico modelo homem-máquina em ergonomia da informática, argumenta-se neste artigo a pertinência da abordagem Situação de Atividade Instrumental- S.A.I. (Rabardel, 1995 para estudar o trabalho informatizado. A aplicação da abordagem S.A.I. para avaliar uma tarefa bancária (Ordem de Pagamento - OP colocou em evidência um conflito de interação instrumental tripolar fornecendo um quadro explicativo aos indicadores de disfuncionamento constatados na análise da atividade dos bancários (n=13Trying to go beyond the classical model of man-machine systems on ergonomics, the aim of this paper is to argue the interest of the Activity Instrument Situation - A.I.S approach (Rabardel, 1995 in computerized work situations. The application of this model on a study of banking task (Payment Order - PO shows a tripolar instrument interaction conflict. Analyzing the activity of bank clerks (n=13, the model brings a framework to explain the dysfunction's indicators

  2. Estudo das interações medicamentosas em prescrições de pacientes de uma Clínica-Escola de Fisioterapia

    Directory of Open Access Journals (Sweden)

    Jean Charles POPETS

    2016-03-01

    Full Text Available Pacientes portadores de doenças crônicas são, em sua maioria, usuários de uma grande quantidade de medicamentos, o que configura polifarmacoterapia. Estes indivíduos apresentam maior possibilidade de sofrerem efeitos nocivos das interações medicamentosas e isso foi tem sido observado em pacientes de uma Clínica-Escola de Fisioterapia no município de Sorocaba. O objetivo desse trabalho foi investigar evidências de interação medicamentosa em prescrições médicas de pacientes da Clínica Escola de Fisioterapia. Foram analisadas as prescrições médicas dos pacientes da área de Neurologia e ortopedia em tratamento de fisioterapia. Os resultados obtidos foram de uma grande variedade de medicamentos em uso por esses pacientes, apresentando de uma até 14 interações entre os medicamentos utilizados. Os fármacos mais utilizados foram na área de cardiologia, com elevada predominância de associações de losartana e hidroclorotiazida, estando presentes em 28,57% das prescrições analisadas. A hipertensão, em conjunto com o diabetes, causa a piora da saúde, aumentando a necessidade de medicamentos a serem usados nestes pacientes. Com base nos resultados foi possível concluir que as principais interações apresentadas nas prescrições dos pacientes da Clínica-Escola de Fisioterapia, no município de Sorocaba, SP, ocorreram entre as classes de anti-hipertensivos, anti-inflamatórios não-esteroidais e hipoglicemiantes, ratificando a necessidade do profissional farmacêutico na avaliação das prescrições, visando o uso racional de medicamentos.

  3. Prednisolone induces the Wnt signalling pathway in 3T3-L1 adipocytes.

    Science.gov (United States)

    Fleuren, Wilco W M; Linssen, Margot M L; Toonen, Erik J M; van der Zon, Gerard C M; Guigas, Bruno; de Vlieg, Jacob; Dokter, Wim H A; Ouwens, D Margriet; Alkema, Wynand

    2013-05-01

    Synthetic glucocorticoids are potent anti-inflammatory drugs but show dose-dependent metabolic side effects such as the development of insulin resistance and obesity. The precise mechanisms involved in these glucocorticoid-induced side effects, and especially the participation of adipose tissue in this are not completely understood. We used a combination of transcriptomics, antibody arrays and bioinformatics approaches to characterize prednisolone-induced alterations in gene expression and adipokine secretion, which could underlie metabolic dysfunction in 3T3-L1 adipocytes. Several pathways, including cytokine signalling, Akt signalling, and Wnt signalling were found to be regulated at multiple levels, showing that these processes are targeted by prednisolone. These results suggest that mechanisms by which prednisolone induce insulin resistance include dysregulation of wnt signalling and immune response processes. These pathways may provide interesting targets for the development of improved glucocorticoids.

  4. Cell shrinkage as a signal to apoptosis in NIH 3T3 fibroblasts

    DEFF Research Database (Denmark)

    Friis, Martin B; Friborg, Christel R; Schneider, Linda

    2005-01-01

    Cell shrinkage is a hallmark of the apoptotic mode of programmed cell death, but it is as yet unclear whether a reduction in cell volume is a primary activation signal of apoptosis. Here we studied the effect of an acute elevation of osmolarity (NaCl or sucrose additions, final osmolarity 687...... mosmol l(-1)) on NIH 3T3 fibroblasts to identify components involved in the signal transduction from shrinkage to apoptosis. After 1.5 h the activity of caspase-3 started to increase followed after 3 h by the appearance of many apoptotic-like bodies. The caspase-3 activity increase was greatly enhanced...... in cells expressing a constitutively active G protein, Rac (RacV12A3 cell), indicating that Rac acts upstream to caspase-3 activation. The stress-activated protein kinase, p38, was significantly activated by phosphorylation within 30 min after induction of osmotic shrinkage, the phosphorylation being...

  5. Tissue transglutaminase expression affects hypusine metabolism in BALB/c 3T3 cells.

    Science.gov (United States)

    Beninati, S; Gentile, V; Caraglia, M; Lentini, A; Tagliaferri, P; Abbruzzese, A

    1998-10-16

    Post-translational formation of hypusine in eukaryotic initiation factor 5A (eIF-5A) is essential for cell viability. Recently, we showed that hypusine protein is an in vitro substrate for transglutaminases (TGases). We report the effect of tissue TGase expression on the in vivo hypusine metabolic pathway. The stable expression of tTGase in BALB/c 3T3 cells induced a 100-fold reduction of hypusine levels and a 50% increase of gamma-glutamyl-omega-hypusine formation. Such changes were paralleled by a consistent decrease in the free polyamine pool and an enhancement of their excretion and of the formation of their gamma-glutamyl derivatives. These effects occurred together with a significant reduction of cell proliferation. In this report we suggest, for the first time, that tTGase affects hypusine metabolism, thus regulating the eIF-5A activity and cell proliferation.

  6. Rectal Imaging: Part I, High-Resolution MRI of Carcinoma of the Rectum at 3 T

    Science.gov (United States)

    Halappa, Vivek Gowdra; Villalobos, Celia Pamela Corona; Bonekamp, Susanne; Gearhart, Susan L.; Efron, Jonathan; Herman, Joseph; Kamel, Ihab R.

    2013-01-01

    OBJECTIVE MRI is currently the imaging modality of choice for the detection, characterization, and staging of rectal cancer. A variety of examinations have been used for preoperative staging of rectal cancer, including digital rectal examination, endorectal (endoscopic) ultrasound, CT, and MRI. Endoscopic ultrasound is the imaging modality of choice for small and small superficial tumors. MRI is superior to CT for assessing invasion to adjacent organs and structures, especially low tumors that carry a high risk of recurrence. CONCLUSION High-resolution MRI is an accurate and sensitive imaging method delineating tumoral margins, mesorectal involvement, nodes, and distant metastasis. In this article, we will review the utility of rectal MRI in local staging, preoperative evaluation, and surgical planning. MRI at 3 T can accurately delineate the mesorectal fascia involvement, which is one of the main decision points in planning treatment. PMID:22733930

  7. Irradiation of Polystyrene and Polypropylene to study NIH 3T3 fibroblasts adhesion

    Energy Technology Data Exchange (ETDEWEB)

    Arbeitman, C.R. [Gerencia de Investigacion y Aplicaciones, TANDAR-CNEA (Argentina); Grosso, M.F. del, E-mail: delgrosso@tandar.cnea.gov.a [Gerencia de Investigacion y Aplicaciones, TANDAR-CNEA (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, CONICET (Argentina); Ibanez, I. [Consejo Nacional de Investigaciones Cientificas y Tecnicas, CONICET (Argentina); Gerencia de Aplicaciones Tecnologicas de la Energia Nuclear, Dpto. de Radiobiologia, TANDAR-CNEA (Argentina); Garcia Bermudez, G. [Gerencia de Investigacion y Aplicaciones, TANDAR-CNEA (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, CONICET (Argentina); Escuela de Ciencia y Tecnologia, UNSAM (Argentina); Duran, H. [Consejo Nacional de Investigaciones Cientificas y Tecnicas, CONICET (Argentina); Gerencia de Aplicaciones Tecnologicas de la Energia Nuclear, Dpto. de Radiobiologia, TANDAR-CNEA (Argentina); Escuela de Ciencia y Tecnologia, UNSAM (Argentina); Chappa, V.C. [Gerencia de Investigacion y Aplicaciones, TANDAR-CNEA (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, CONICET (Argentina); Mazzei, R. [U.A. Tecnologicas y Agropecuarias, CNEA, Dpto. Ing. Quimica, UTN FRBA, Bs. As. (Argentina); Behar, M. [Instituto de Fisica, Universidade Federal do Rio Grande do Sul, Porto Alegre (Brazil)

    2010-10-01

    When polymers are irradiated with heavy ions new chemical groups are created in a few microns of the material. The irradiation changed the polarity and wettability on the surface so that could enhance the biocompatibility of the modified polymer. The study of chemistry and nanoscale topography of the biomaterial is important in determining its potential applications in medicine and biotechnology, because their strong influence on cell function, adhesion and proliferation. In this study, thin films of Polystyrene and Polypropylene samples were modified by irradiation with low energy ion beams (30-150 keV) and swift heavy ions both with various fluences and energies. The changes were evaluated with different methods. Adhesion of NIH 3T3 fibroblasts onto unirradiated and irradiated surfaces has been studied by in vitro techniques. The correlations between physicochemical properties as a function of different irradiations parameters were compared with cell adhesion on the modified polymer surface.

  8. Fructose promotes the differentiation of 3T3-L1 adipocytes and accelerates lipid metabolism.

    Science.gov (United States)

    Legeza, Balázs; Balázs, Zoltán; Odermatt, Alex

    2014-01-31

    Excessive fructose consumption and elevated glucocorticoids contribute to metabolic syndrome. We show that fructose as the only carbohydrate source is sufficient for the differentiation of 3T3-L1 fibroblasts into adipocytes. Differentiation of cells in fructose containing medium resulted in increased 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) expression and activity. Experiments with transfected HEK-293 cells suggested more efficient NADPH generation by fructose compared with glucose in the endoplasmic reticulum (ER). Adipocytes differentiated in the presence of fructose showed increased FABP4 expression, C/EBPα to C/EBPβ ratio and lipolysis. Thus, excessive fructose may cause adverse metabolic effects by enhancing 11β-HSD1 activity and increasing lipolysis in adipocytes. Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

  9. Finite-Temperature Phase Diagram of the d=3 tJ Model with Quenched Disorder

    Science.gov (United States)

    Berker, A. Nihat; Hinczewski, Michael

    2008-03-01

    We study a quenched disordered d=3 tJ Hamiltonian with static vacancies as a model of nonmagnetic impurities in high-Tc materials.[1,2] Using a position-space renormalization-group approach, we calculate the evolution of the finite-temperature phase diagram with impurity concentration p, and find several features with close experimental parallels: away from half-filling we see the rapid destruction of a spin-singlet liquid phase (analogous to the superconducting phase in cuprates) which is eliminated for p >=0.05; in the same region for these dilute impurity concentrations we observe an enhancement of antiferromagnetism. The antiferromagnetic phase near half-filling is robust against impurity addition, and disappears only for p >=0.40. [1] M. Hinczewski and A.N. Berker, Eur. Phys. J. B 51, 461 (2006). [2] M. Hinczewski and A.N. Berker, arXiv:cond-mat/0607171v1 [cond-mat.str-el].

  10. Genetic Analysis and Mapping of Dominant Minute Grain Gene Mi3(t) in Rice

    Institute of Scientific and Technical Information of China (English)

    LIU Ming-wei; LIU Yong; WANG Shi-quan; DENG Qi-ming; LI Ping

    2005-01-01

    Grain size, determined chiefly by grain Iength, is one of the main factors affecting the grain yield in rice production. To study the trait of rice grain size, F1 and F2 populations were developed from crosses Shuhui 881/Y34 and Shuhui 527/Y34, and genetic analysis for minute grain was performed. The F1 populations showed minute grains, and grain size segregations in the two F2 populations were both in accordance with the ratio of 3:1, indicating that minute grain in Y34 was controlled by a completely dominant gene. By using the F2 population from Shuhui 881/Y34, this dominant gene, tentatively designated as Mi3(t), was mapped based on SSR markers in the interval between RM282 (genetic distance of 5.1 cM) and RM6283 (genetic distance of 0.9 cM) on the short arm of chromosome 3.

  11. Hormone and pharmaceutical regulation of ASP production in 3T3-L1 adipocytes.

    Science.gov (United States)

    Gao, Ying; Gauvreau, Danny; Cianflone, Katherine

    2010-04-01

    Several studies have demonstrated increases in acylation stimulating protein (ASP), and precursor protein C3 in obesity, diabetes and dyslipidemia, however the nature of the regulation is unknown. To evaluate chronic hormonal and pharmaceutical mediated changes in ASP and potential mechanisms, 3T3-L1 adipocytes were treated with physiological concentrations of relevant hormones and drugs currently used in treatment of metabolic diseases for 48 h. Medium ASP production and C3 secretion were evaluated in relation to changes in adipocyte lipid metabolism (cellular triglyceride (TG) mass, non-esterified fatty acid (NEFA) release and real-time FA uptake). Chylomicrons increased ASP production (up to 411 +/- 133% P ASP production (-53 to -85%, P ASP (-54% to -100%, P ASP were also associated with decreased TG mass (maximal -60%, P ASP profiles in subjects, and suggest that ASP may be regulated through precursor C3 availability, convertase activity and differentiation status. Copyright 2010 Wiley-Liss, Inc.

  12. Epigenetic Modification Mediates the Increase of LAG-3(+) T Cells in Chronic Osteomyelitis.

    Science.gov (United States)

    Wang, Yicun; Wang, Jun; Meng, Jia; Jiang, Hui; Zhao, Jianning; Qian, Hongbo; Chen, Tao

    2016-12-27

    Immune suppression plays critical roles in the development of chronic osteomyelitis, and the mechanisms underlying the development of immune suppression in chronic osteomyelitis have attracted much attention. LAG-3 is an important suppressor of T cell activation, but the role of LAG-3 in the immune regulation of chronic osteomyelitis is currently unknown. We sought to demonstrate if LAG-3 plays crucial roles in chronic osteomyelitis progression and has effects on immune suppression and exhausting of T cells, and what is the mechanism underlying LAG-3 deregulation in chronic osteomyelitis. We examined the expression of LAG-3 in the T cells of peripheral blood of 50 healthy controls and 50 patients with chronic osteomyelitis by flow cytometry. Clinical data were analyzed to determine the correlation between inflammation index and LAG-3 expression. Moreover, we isolated the CD4(+) T cells from healthy controls and chronic osteomyelitis patients to compare cell proliferation and IFN-γ production. Chromatin immunoprecipitation assays were utilized to analyze the epigenetic modification on LAG-3 expression in T cells. We found that LAG-3 was significantly increased in the T cells of peripheral blood from chronic osteomyelitis patients. Subsequently, clinical data analysis suggested that the higher expression of LAG-3 was associated with severer inflammation situation. Consistently, LAG-3(+)CD4(+) T cells exhibited impaired cell proliferation and IFN-γ secretion. Deregulation of histone methylation mediated the increase of LAG-3(+) T cells during chronic osteomyelitis. Taken together, our study demonstrates the increase of LAG-3(+) T cells and its immune regulatory roles in chronic osteomyelitis progression, suggesting new mechanisms and potential therapeutic targets for chronic osteomyelitis.

  13. Proton MR spectroscopy of cerebral gliomas at 3 T: spatial heterogeneity, and tumour grade and extent

    Energy Technology Data Exchange (ETDEWEB)

    Di Costanzo, Alfonso; Catapano, Domenico; D' Angelo, Vincenzo A. [Department of Health Sciences, University of Molise, Campobasso (Italy); Scarabino, Tommaso; Popolizio, Teresa; Giannatempo, Giuseppe M. [Department of Neuroradiology, Scientific Institute, Foggia (Italy); Trojsi, Francesca; Bonavita, Simona; Tedeschi, Gioacchino [Department of Neurological Sciences, 2. University of Naples (Italy); Portaluri, Maurizio [Department of Radiotherapy, Perrino Hospital, Brindisi (Italy); Tosetti, Michela [Department of Magnetic Resonance, Scientific Institute Stella Maris, Pisa (Italy); Salvolini, Ugo [Department of Radiology, Polytechnic University of Marches, Ancona (Italy)

    2008-08-15

    This study aimed to evaluate the usefulness of proton MR spectroscopic imaging ({sup 1}H-MRSI) at 3 T in differentiating high- from low-grade gliomas, and tumour from necrosis, oedema or normal tissue. Forty-four patients with brain gliomas and four with meningiomas were retrospectively reviewed. The normalised metabolites choline (nCho), N-acetylaspartate (nNAA), creatine (nCr) and lactate/lipids (nLL), and the metabolite ratios Cho/NAA, NAA/Cr and Cho/Cr were calculated. Necrotic-appearing areas showed two spectroscopic patterns: 'necrosis' with variable nCho and high nLL, and 'cystic necrosis' with variable nLL or nonevident peaks. Peri-enhancing oedematous-appearing areas showed three spectroscopic patterns ('tumour' with abnormal Cho/NAA, 'oedema' with normal Cho/NAA and 'tumour/oedema' with normal nCho and abnormal Cho/NAA) in gliomas, and one ('oedema') in meningiomas. Peri-enhancing or peri-tumour normal-appearing areas showed two patterns ('infiltrated' with abnormal nCho and/or Cho/NAA and 'normal' with normal spectra) in gliomas and one ('normal') in meningiomas. Discriminant analysis showed that classification accuracy between high- and low-grade glioma masses was better with normalised metabolites or all parameters together than metabolite ratios and that among peri-enhancing areas was much better with normalised metabolites. The analysis of spatial distribution of normalised metabolites by 3-T {sup 1}H-MRSI helps to discriminate among different tissues, offering information not available with conventional MRI. (orig.)

  14. Superior Cervical Sympathetic Ganglion: Normal Imaging Appearance on 3T-MRI

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Joo Yeon; Lee, Jeong Hyun [Department of Radiology and Research Institute of Radiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505 (Korea, Republic of); Song, Joon Seon; Song, Min Jeong [Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505 (Korea, Republic of); Hwang, Seung-Jun [Department of Anatomy and Cell Biology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505 (Korea, Republic of); Yoon, Ra Gyoung [Department of Radiology, Catholic Kwandong University International St. Mary' s Hospital, Catholic Kwandong University College of Medicine, Incheon 22711 (Korea, Republic of); Jang, Seung Won; Park, Ji Eun [Department of Radiology and Research Institute of Radiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505 (Korea, Republic of); Heo, Young Jin [Department of Radiology, Busan Paik Hospital, Inje University College of Medicine, Busan 47392 (Korea, Republic of); Choi, Young Jun; Baek, Jung Hwan [Department of Radiology and Research Institute of Radiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505 (Korea, Republic of)

    2016-11-01

    To identify superior cervical sympathetic ganglion (SCSG) and describe their characteristic MR appearance using 3T-MRI. In this prospective study, we recruited 53 consecutive patients without history of head and neck irradiation. Using anatomic location based on literature review, both sides of the neck were evaluated to identify SCSGs in consensus. SCSGs were divided into definite (medial to internal carotid artery [ICA] and lateral to longus capitis muscle [LCM]) and probable SCSGs based on relative location to ICA and LCM. Two readers evaluated signal characteristics including intraganglionic hypointensity of all SCSGs and relative location of probable SCSGs. Interrater and intrarater agreements were quantified using unweighted kappa. Ninety-one neck sites in 53 patients were evaluated after exclusion of 15 neck sites with pathology. Definite SCSGs were identified at 66 (73%) sites, and probable SCSGs were found in 25 (27%). Probable SCSGs were located anterior to LCM in 16 (18%), lateral to ICA in 6 (7%), and posterior to ICA in 3 (3%). Intraganglionic hypointensity was identified in 82 (90%) on contrast-enhanced fat-suppressed T1-weighted images. There was no statistical difference in the relative location between definite and probable SCSGs of the right and left sides with intragnalionic hypointensity on difference pulse sequences. Interrater and intrarater agreements on the location and intraganglionic hypointensity were excellent (κ-value, 0.749–1.000). 3T-MRI identified definite SCSGs at 73% of neck sites and varied location of the remaining SCSGs. Intraganglionic hypointensity was a characteristic feature of SCSGs.

  15. Superior cervical sympathetic ganglion: Normal imaging appearance on 3T-MRI

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Joo Yeon; Lee, Jeong Hyun; Song, Joon Seon [Dept. of Radiology, and Research Institute of Radiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul (Korea, Republic of); and others

    2016-09-15

    To identify superior cervical sympathetic ganglion (SCSG) and describe their characteristic MR appearance using 3T-MRI. In this prospective study, we recruited 53 consecutive patients without history of head and neck irradiation. Using anatomic location based on literature review, both sides of the neck were evaluated to identify SCSGs in consensus. SCSGs were divided into definite (medial to internal carotid artery [ICA] and lateral to longus capitis muscle [LCM]) and probable SCSGs based on relative location to ICA and LCM. Two readers evaluated signal characteristics including intraganglionic hypointensity of all SCSGs and relative location of probable SCSGs. Interrater and intrarater agreements were quantified using unweighted kappa. Ninety-one neck sites in 53 patients were evaluated after exclusion of 15 neck sites with pathology. Definite SCSGs were identified at 66 (73%) sites, and probable SCSGs were found in 25 (27%). Probable SCSGs were located anterior to LCM in 16 (18%), lateral to ICA in 6 (7%), and posterior to ICA in 3 (3%). Intraganglionic hypointensity was identified in 82 (90%) on contrast-enhanced fat-suppressed T1-weighted images. There was no statistical difference in the relative location between definite and probable SCSGs of the right and left sides with intragnalionic hypointensity on difference pulse sequences. Interrater and intrarater agreements on the location and intraganglionic hypointensity were excellent (κ-value, 0.749–1.000). 3T-MRI identified definite SCSGs at 73% of neck sites and varied location of the remaining SCSGs. Intraganglionic hypointensity was a characteristic feature of SCSGs.

  16. Chronic kidney disease: pathological and functional assessment with diffusion tensor imaging at 3T MR

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Zhiling; Zhang, Jie; Cai, Shifeng; Yuan, Xianshun; Liu, Qingwei [Shandong University, Department of Radiology, Provincial Hospital Affiliated to Shandong University, Jinan (China); Xu, Ying; Wang, Rong [Shandong University, Department of Nephrology, Provincial Hospital Affiliated to Shandong University, Jinan (China); Zhen, Junhui [Shandong University, Department of Pathology, Qilu Hospital of Shandong University, Jinan (China)

    2014-10-11

    Our objective was to evaluate pathological and functional changes in chronic kidney disease (CKD) using diffusion tensor imaging (DTI) at 3 T. There were fifty-one patients with CKD who required biopsy and 19 healthy volunteers who were examined using DTI at 3 T. The mean values of fractional anisotropy (FA) and the apparent diffusion coefficient (ADC) were obtained from the renal parenchyma (cortex and medulla). Correlations between imaging results and the estimated glomerular filtration rate (eGFR), as well as pathological damage (glomerular lesion and tubulointerstitial injury), were evaluated. The renal cortical FA was significantly lower than the medullary in both normal and affected kidneys (p < 0.001). The parenchymal FA was significantly lower in patients than healthy controls, regardless of whether eGFR was reduced. There were positive correlations between eGFR and FA (cortex, r = 0.689, p = 0.000; and medulla, r = 0.696, p = 0.000), and between eGFR and ADC (cortex, r = 0.310, p = 0.017; and medulla, r = 0.356, p = 0.010). Negative correlations were found between FA and the glomerular lesion (cortex, r = -0.499, p = 0.000; and medulla, r = -0.530, p = 0.000), and between FA and tubulointerstitial injury (cortex, r = -0.631, p = 0.000; and medulla, r = -0.724, p = 0.000). DTI is valuable for noninvasive assessment of renal function and pathology in patients with CKD. A decrease in FA could identify the glomerular lesions, tubulointerstitial injuries, and eGFR. (orig.)

  17. Melatonin Suppresses Autophagy Induced by Clinostat in Preosteoblast MC3T3-E1 Cells.

    Science.gov (United States)

    Yoo, Yeong-Min; Han, Tae-Young; Kim, Han Sung

    2016-04-08

    Microgravity exposure can cause cardiovascular and immune disorders, muscle atrophy, osteoporosis, and loss of blood and plasma volume. A clinostat device is an effective ground-based tool for simulating microgravity. This study investigated how melatonin suppresses autophagy caused by simulated microgravity in preosteoblast MC3T3-E1 cells. In preosteoblast MC3T3-E1 cells, clinostat rotation induced a significant time-dependent increase in the levels of the autophagosomal marker microtubule-associated protein light chain (LC3), suggesting that autophagy is induced by clinostat rotation in these cells. Melatonin treatment (100, 200 nM) significantly attenuated the clinostat-induced increases in LC3 II protein, and immunofluorescence staining revealed decreased levels of both LC3 and lysosomal-associated membrane protein 2 (Lamp2), indicating a decrease in autophagosomes. The levels of phosphorylation of mammalian target of rapamycin (p-mTOR) (Ser2448), phosphorylation of extracellular signal-regulated kinase (p-ERK), and phosphorylation of serine-threonine protein kinase (p-Akt) (Ser473) were significantly reduced by clinostat rotation. However, their expression levels were significantly recovered by melatonin treatment. Also, expression of the Bcl-2, truncated Bid, Cu/Zn- superoxide dismutase (SOD), and Mn-SOD proteins were significantly increased by melatonin treatment, whereas levels of Bax and catalase were decreased. The endoplasmic reticulum (ER) stress marker GRP78/BiP, IRE1α, and p-PERK proteins were significantly reduced by melatonin treatment. Treatment with the competitive melatonin receptor antagonist luzindole blocked melatonin-induced decreases in LC3 II levels. These results demonstrate that melatonin suppresses clinostat-induced autophagy through increasing the phosphorylation of the ERK/Akt/mTOR proteins. Consequently, melatonin appears to be a potential therapeutic agent for regulating microgravity-related bone loss or osteoporosis.

  18. Resveratrol induces apoptosis and inhibits adipogenesis in 3T3-L1 adipocytes.

    Science.gov (United States)

    Rayalam, Srujana; Yang, Jeong-Yeh; Ambati, Suresh; Della-Fera, Mary Anne; Baile, Clifton A

    2008-10-01

    Resveratrol, a phytoallexin, has recently been reported to slow aging by acting as a sirtuin activator. Resveratrol also has a wide range of pharmacological effects on adipocytes. In this study, we investigated the effects of resveratrol on adipogenesis and apoptosis using 3T3-L1 cells. In mature adipocytes, 100 and 200 microM resveratrol decreased cell viability dose-dependently by 23 +/- 2.7%, and 75.3 +/- 2.8% (p < 0.0001), respectively, after 48 h treatment, and 100 microM resveratrol increased apoptosis by 76 +/- 8.7% (p < 0.0001). Resveratrol at 25 and 50 microM decreased lipid accumulation in maturing preadipocytes significantly by 43 +/- 1.27% and 94.3 +/- 0.3% (p < 0.0001) and decreased cell viability by 25 +/- 1.3% and 70.4 +/- 1.6% (p < 0.0001), respectively. In order to understand the anti-adipogenic effects of resveratrol, maturing 3T3-L1 preadipocytes were treated with 25 microM resveratrol and the change in the expression of several adipogenic transcription factors and enzymes was investigated using real-time RT-PCR. Resveratrol down-regulated the expression of PPAR gamma, C/EBP alpha, SREBP-1c, FAS, HSL, LPL and up-regulated the expression of genes regulating mitochondrial activity (SIRT3, UCP1 and Mfn2). These results indicate that resveratrol may alter fat mass by directly affecting cell viability and adipogenesis in maturing preadipocytes and inducing apoptosis in adipocytes and thus may have applications for the treatment of obesity.

  19. The utility of high b-value DWI in evaluation of ischemic stroke at 3 T

    Energy Technology Data Exchange (ETDEWEB)

    Cihangiroglu, Mutlu, E-mail: mcihangiroglu@yeditepe.edu.tr [Department of Radiology, Yeditepe University Hospital, Istanbul (Turkey); Citci, Beyza, E-mail: bcitci@yeditepe.edu.tr [Department of Neurology, Yeditepe University Hospital, Istanbul (Turkey); Kilickesmez, Ozgur, E-mail: okilickesmez@yeditepe.edu.tr [Department of Radiology, Yeditepe University Hospital, Istanbul (Turkey); Firat, Zeynep, E-mail: zfirat@yeditepe.edu.tr [Department of Radiology, Yeditepe University Hospital, Istanbul (Turkey); Karlikaya, Geysu, E-mail: gkarlikaya@yeditepe.edu.tr [Department of Neurology, Yeditepe University Hospital, Istanbul (Turkey); Ulug, Aziz M., E-mail: ulug@ieee.org [Department of Biomedical Engineering, Yeditepe University, Istanbul (Turkey); The Feinstein Institute for Medical Research, Manhasset, NY (United States); Bingol, Canan A., E-mail: caykutbingol@yeditepe.edu.tr [Department of Neurology, Yeditepe University Hospital, Istanbul (Turkey); Kovanlikaya, Ilhami, E-mail: ilk2002@med.cornell.edu [Department of Radiology, Weill Cornell Medical College, NY (United States)

    2011-04-15

    Purpose: The utility of DWI with high b-value in ischemic stroke is still unsettled. The purpose of this study is to compare high b-value (3000) and standard b-value (1000) diffusion-weighted images in patients with ischemic stroke at 3 T. Materials and methods: 27 patients with acute stroke who were admitted to the hospital during the first 24 h after symptom onset were included in this study. All patients had a brain MRI study with stroke protocol including standard (b = 1000) DWI and high b-value (b = 3000) DWI sequences at 3 T MR scanner. Number and localization of the lesions were assessed MR signal intensities (SI), signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), contrast ratio (CR) and apparent diffusion coefficient (ADC) values of the lesions and normal parenchyma on DWI with b = 1000 and b = 3000 sequences were measured. Results: All patients with acute stroke revealed hyperintense lesions due to restricted diffusion on DWI with both b-values. However, lesions of restricted diffusion were more conspicuous in b = 3000 value DWI than b = 1000, and additional 4 ischemic lesions were detected on b = 3000 DWI. SNR, CNR, SI and also ADC values in both stroke area and normal parenchyma were lower at b = 3000 than the value at b = 1000. At b = 3000, CR was significantly greater than b = 1000 images. Conclusions: Although quantitative analysis shows higher SI, SNR and CNR values with standard b-value (b = 1000) diffusion-weighted imaging, using higher b-value may still be beneficial in detecting additional subtle lesions in patients whose clinical findings are not correlated with standard b-value DWI in stroke.

  20. As interações entre os atores no retorno ao trabalho após afastamento por transtorno mental: uma metaetnografia

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    Robson da Fonseca Neves

    2015-11-01

    Full Text Available Resumo Transtornos mentais repercutem no mundo do trabalho. Estudos sobre interações entre os atores envolvidos no retorno ao trabalho são raros. A metaetnografia presta-se a sintetizar estudos qualitativos através da interpretação e comparação contínua dos conceitos presentes nos artigos. Este estudo propõe uma metaetnografia sobre as interações entre os atores sociais envolvidos no processo de retorno ao trabalho após afastamento por transtornos mentais. Visa: (1 explorar as interações entre os atores sociais envolvidos no retorno ao trabalho; (2 identificar facilitadores ou obstáculos para o retorno ao trabalho. A busca nas bases de dados produziu 619 artigos dos quais 16 atenderam aos critérios de inclusão. A análise dos artigos revelou seis conceitos de segunda ordem que resultaram em duas sínteses. A primeira diz respeito ao ethos do desempenho no retorno ao trabalho e a segunda aponta para o retorno ao trabalho como catalizador de novos modos de vida. Modelos que privilegiam o ethos do desempenho do trabalhador, bem como uma perspectiva orientada por aspectos psicossociais podem facilitar as praticas de retorno ao trabalho após afastamento por transtornos mentais.

  1. A interação entre crianças surdas no contexto de uma escola de Educação Infantil

    Directory of Open Access Journals (Sweden)

    Erica Aparecida Garrutti de Lourenço

    2013-01-01

    Full Text Available Este artigo objetiva conhecer como se dá interação entre crianças surdas na Educação Infantil. A metodologia pautou-se na observação em campo de uma turma de Educação Infantil em uma escola especializada na Educação de Surdos da Rede Municipal de São Paulo. Foram realizadas também entrevistas com a professora da turma observada e a coordenadora. Como outros procedimentos de coleta de dados, analisou-se o Projeto Político Pedagógico da escola e realizou-se uma atividade dirigida com as crianças. Os resultados revelaram que as interações entre as crianças surdas ocorrem naturalmente, visto que essas crianças compartilham de uma mesma língua, a Língua de Sinais e da mesma cultura. A escola pesquisada tem como meta respeitar a língua, a cultura e a identidade surda, tendo a preocupação em centrar-se no uso da LIBRAS como forma de comunicação para o favorecimento da aprendizagem da criança surda.Palavras-chave: Educação de surdos; Interação entre crianças; EducaçãoInfantil; Bilinguismo.

  2. A aprendizagem coletiva de língua portuguesa para surdos através das interações em língua de sinais

    Directory of Open Access Journals (Sweden)

    Vanessa de Oliveira Dagostim Pires

    Full Text Available Este artigo analisa as interações em língua de sinais (LS, entre alunos surdos do ensino fundamental, nas aulas de Língua Portuguesa. Ele foi inspirado no trabalho de Donato (1994, que buscou identificar a presença da prática do andaimento nas interações entre pares em sala de aula de língua francesa como segunda língua. Em nossa pesquisa, analisamos através do sociointeracionismo (VYGOTSKY, 1988; WOOD, BRUNNER e ROSS, 1976 as interações entre os alunos surdos durante o desenvolvimento de uma unidade didática que os incentivava a escrever uma carta de reclamação, em Língua Portuguesa, sobre a sua escola. É possível perceber como as estratégias de andaimento possibilitaram a construção coletiva de aprendizagem para a realização da tarefa proposta.

  3. Repertórios de interação estado-sociedade em um estado heterogêneo: a experiência na Era Lula

    Directory of Open Access Journals (Sweden)

    Rebecca Abers

    2014-06-01

    Full Text Available Este artigo analisa a combinação de rotinas de interação do Estado com os movimentos sociais na construção de políticas públicas durante o governo Lula em três setores de políticas: desenvolvimento agrário, política urbana e segurança pública. O argumento central é que, em um contexto caracterizado por uma permeabilidade inédita do Estado, movimentos sociais e atores estatais experimentaram criativamente padrões históricos de interação Estado-sociedade. Sob o mote da "participação da sociedade civil", movimentos sociais e atores estatais recorreram a um repertório de interação diversificado, que incluiu a participação institucional, protestos, ocupação de cargos na burocracia pública e relações personalizadas, com ênfase variada a depender dos padrões pretéritos de relação Estado-sociedade em cada setor.

  4. A influência do discurso do professor na motivação e na interação social em sala de aula

    Directory of Open Access Journals (Sweden)

    Marco Aurélio Alvarenga Monteiro

    2012-01-01

    Full Text Available Estudos têm mostrado a importância das interações sociais desencadeadas em sala de aula como fundamentais para a aprendizagem dos alunos. Contudo, o professor deve ser capaz de motivar os estudantes para se envolverem mais ativamente com as atividades de ensino propostas. Este artigo apresenta os resultados de uma investigação sobre as interações sociais desenvolvidas no contexto de uma sala de aula do Ensino Médio, que explicou os princípios da conservação da energia mecânica com uma aula experimental de demonstração. O principal objetivo era entender como o discurso do professor pode contribuir para o bom nível de motivação do aluno durante uma aula. As entrevistas com professor e alunos e todo o processo de interação durante as atividades foram gravados, transcritos e analisados . Os resultados ressaltam a importância da abordagem discursiva dos professores para manter o processo de motivação entre os alunos.

  5. Diagnostic accuracy of 3T conventional shoulder MRI in the detection of the long head of the biceps tendon tears associated with rotator cuff tendon tears

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Ro Woon; Choi, Soo-Jung; Ahn, Jae Hong; Shin, Dong Rock; Kang, Chae Hoon [University of Ulsan, College of Medicine, Department of Radiology, Asan Foundation, Gangneung Asan Hospital, Gangneung-si, Gangwon-do (Korea, Republic of); Lee, Man Ho [Andong Medical Center, Department of Radiology, Andong-si, Gyeongsangbuk-do (Korea, Republic of); Lee, Ki Won [University of Ulsan, College of Medicine, Department of Orthopedic Surgery, Asan Foundation, Gangneung Asan Hospital, Gangneung-si, Gangwon-do (Korea, Republic of)

    2016-12-15

    To evaluate the diagnostic performance (DP) of 3T (3 Tesla field strength) conventional shoulder magnetic resonance imaging (MRI) in detecting the long head of the biceps tendon (LHBT) tears in association with rotator cuff tendon tears. This study included 80 consecutive patients who underwent arthroscopic surgery for rotator cuff tendon tears. Two radiologists independently evaluated the preoperative 3T shoulder MRI for the presence of LHBT tears. The DP of MRI was evaluated using the results of arthroscopy as the reference standard. We also evaluated the DP of several MR signs of LHBT in detection of partial LHBT tears. Arthroscopic examination revealed 35 partial and 5 complete tears. According to the results of evaluation by reviewers 1 and 2, shoulder MRI exhibited sensitivities of 77.14 and 80 % and specificities of 71.11 and 73.33 % in detection of partial LHBT tears and sensitivities of 80 and 100 % and a specificity of 100% (both) in detection of complete LHBT tears. In detecting partial LHBT tears, increased T2 signal intensity of the LHBT exhibited high sensitivities (reviewers 1 and 2; 82.85 and 80 %, respectively) and the presence of intratendinous defects or C-signs exhibited the highest specificities (reviewers 1 and 2; 95.55 and 93.33 %, respectively), followed by abnormalities in shape and outer margins of the LHBT (reviewers 1 and 2; 91.11 and 82 %; 91.11 and 86.66 %, respectively). Non-contrast-enhanced 3T shoulder MRI is potentially highly accurate in detection of complete LHBT tears, but moderately accurate in detection of partial LHBT tears. (orig.)

  6. Coptis chinensis alkaloids exert anti-adipogenic activity on 3T3-L1 adipocytes by downregulating C/EBP-α and PPAR-γ.

    Science.gov (United States)

    Choi, Jae Sue; Kim, Ji-Hye; Ali, Md Yousof; Min, Byung-Sun; Kim, Gun-Do; Jung, Hyun Ah

    2014-10-01

    Obesity is a complex, multifactorial, and chronic disease that increases the risk for type 2 diabetes, coronary heart disease and hypertension, and has become a major worldwide health problem. Developing novel anti-obesity drugs from natural products is a promising solution to the global health problem of obesity. While screening anti-obesity potentials of natural products, the methanol extract of the rhizome of Coptis chinensis (Coptidis Rhizoma) was found to significantly inhibit adipocyte differentiation and lipid contents in 3T3-L1 cells, as assessed by Oil-Red O staining. Five known alkaloids, berberine, epiberberine, coptisine, palmatine, and magnoflorine, were isolated from the n-BuOH fraction of the methanol extract of Coptidis Rhizoma. We determined the chemical structure of these alkaloids through comparisons of published nuclear magnetic resonance (NMR) spectral data. Furthermore, we screened these alkaloids for their ability to inhibit adipogenesis over a range of concentrations (12.5-50 μM). All five Coptidis Rhizoma alkaloids significantly inhibited lipid accumulation in 3T3-L1 cells without affecting cell viability in a concentration dependent manner. In addition, the five alkaloids significantly reduced the expression levels of several adipocyte marker genes including proliferator activated receptor-γ (PPAR-γ) and CCAAT/enhancer-binding protein-α (C/EBP-α). In the present study, we found that the isolated alkaloids inhibited adipogenesis in a dose-dependent manner in 3T3-L1 cells; this inhibition was attributed to their abilities to downregulate the protein levels of the adipocyte marker proteins PPAR-γ and C/EBP-α. Thus, these results suggest that Coptidis Rhizoma extract and its isolated alkaloids may be of therapeutic interest with respect to the treatment of obesity.

  7. 3D-FIESTA MRI at 3 T demonstrating branches of the intraparotid facial nerve, parotid ducts and relation with benign parotid tumours.

    Science.gov (United States)

    Li, Chuanting; Li, Yan; Zhang, Dongsheng; Yang, Zhenzhen; Wu, Lebin

    2012-11-01

    To investigate the usefulness of three-dimensional (3D) fast imaging employing steady state precession (FIESTA) magnetic resonance imaging (MRI) at 3 T in evaluating the intraparotid components of the facial nerve and parotid ducts, and to compare the MRI images with surgical findings. Thirty-one cases of benign parotid tumours were studied with conventional and 3D FIESTA MRI sequences at 3T using a head coil. The most clinically useful 3D FIESTA images were acquired at parameters of 4.9 ms repetition time (TR); 1.5 effective echo time (TEeff); a flip of 55°, a field of view of 18 to 20 cm, a matrix of 512 × 320, an axial plane, no gaps, and a section thickness of 1 mm. Post-processed multiplanar images were obtained with an Advantage Windows (AW sdc 4.3) workstation. Parotid ducts, facial nerves, and tumours were identified on these images. The relationship of the tumours to the facial nerves and parotid ducts was confirmed at surgery. The facial nerves appeared as linear structures of low intensity. The main trunk of the facial nerve was identified bilaterally in 93.5% of the 3D-FIESTA sequence images. Parotid ducts appeared as structures of high intensity on multiplanar 3D-FIESTA images (100%). The relationships of the tumours with the cervicofacial and temporofacial divisions of the facial nerve were correctly diagnosed in 26 of 31 cases (83.9%) using 3D-FIESTA sequence images. 3D-FIESTA MRI at 3 T depicted the main trunk, cervicofacial and temporofacial divisions of the facial nerve, and the main parotid duct. It is useful for preoperative evaluation of parotid gland tumours. Copyright © 2012 The Royal College of Radiologists. Published by Elsevier Ltd. All rights reserved.

  8. Prevalência e significância clínica de interações fármaco-nutrição enteral em Unidades de Terapia Intensiva

    Directory of Open Access Journals (Sweden)

    Adriano Max Moreira Reis

    2014-01-01

    Full Text Available Estudo multicêntrico, transversal e retrospectivo, que objetivou determinar a prevalência de interações fármaco-nutrição enteral (NE em Unidades de Terapia Intensiva (UTI de sete hospitais de ensino do Brasil, e analisar a significância clínica das mesmas. Foram coletadas informações sobre medicamentos e NE administrados com 24 horas e 120 horas de internação. Para identificação das interações fármaco-NE empregou-se o software Drug Reax®. Foram investigados 1.124 prontuários. Destes, 320 pacientes, com 24 horas de internação, estavam em uso de NE, sendo que 20 (6,3% apresentaram interação fármaco-NE. Dos 504 pacientes, com 120 horas de internação, 39 (7,7% apresentaram interação fármaco-NE. As potenciais interações fármaco-NE mais frequentes foram: fenitoína-NE, levotiroxina-NE e varfarina-NE. As interações fármaco-NE podem interferir na qualidade e no custo da assistência prestada em UTI e, desta forma, é essencial que a equipe de saúde tenha conhecimento sobre as mesmas.

  9. Feasibility of free-breathing, GRAPPA-based, real-time cardiac cine assessment of left-ventricular function in cardiovascular patients at 3T.

    Science.gov (United States)

    Zhu, Xiaomei; Schwab, Felix; Marcus, Roy; Hetterich, Holger; Theisen, Daniel; Kramer, Harald; Notohamiprodjo, Mike; Schlett, Christopher L; Nikolaou, Konstantin; Reiser, Maximilian F; Bamberg, Fabian

    2015-05-01

    To determine the feasibility of free-breathing, GRAPPA-based, real-time (RT) cine 3T cardiac magnetic resonance imaging (MRI) with high acceleration factors for the assessment of left-ventricular function in a cohort of patients as compared to conventional segmented cine imaging. In this prospective cohort study, subjects with various cardiac conditions underwent MRI involving two RT cine sequences (high resolution and low resolution) and standard segmented cine imaging. Standard qualitative and quantitative parameters of left-ventricular function were quantified. Among 25 subjects, 24 were included in the analysis (mean age: 50.5±21 years, 67% male, 25% with cardiomyopathy). RT cine derived quantitative parameters of volumes and left ventricular mass were strongly correlated with segmented cine imaging (intraclass correlation coefficient [ICC]: >0.72 for both RT cines) but correlation for peak ejection and filling rates were moderate to poor for both RT cines (ICCcines significantly underestimated peak ejection and filling rates (>103.2±178 ml/s). Among patient-related factors, heart rate was strongly predictive for deviation of measurements (pcine MRI at 3T is feasible for qualitative and quantitative assessment of left ventricular function for low and high-resolution sequences but results in significant underestimation of systolic function, peak ejection and filling rates. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  10. Adsorption kinetics for the removal of copper(II from aqueous solution by adsorbent PSTM-3T

    Directory of Open Access Journals (Sweden)

    G Burmaa

    2014-09-01

    Full Text Available The batch removal of copper (II from aqueous solution under different experimental conditions using silicon-organic sorbent poly[N,N’-bis(3-silseskquioxanilpropylthiocarbamide] (PSTM-3T was investigated in this study. This sorbent was produced from the hydrolytic poly-condensation reaction. The removal was favoured at pH = 5 for PSTM-3T. The effects of concentration and temperature have been reported. PSTM-3T was found to efficiently remove Cu(II from solution. The batch sorption kinetics have been tested for a first-order reaction. The rate constants of adsorption have been calculated. The thermodynamic parameters (ΔG0, Kc obtained to indicate the endothermic nature of Cu(II adsorption on PSTM-3T.DOI: http://dx.doi.org/10.5564/mjc.v12i0.162 Mongolian Journal of Chemistry Vol.12 2011: 1-6

  11. Acute vertigo in an anesthesia provider during exposure to a 3T MRI scanner.

    Science.gov (United States)

    Gorlin, Andrew; Hoxworth, Joseph M; Pavlicek, William; Thunberg, Christopher A; Seamans, David

    2015-01-01

    Vertigo induced by exposure to the magnetic field of a magnetic resonance imaging (MRI) scanner is a well-known phenomenon within the radiology community but is not widely appreciated by other clinical specialists. Here, we describe a case of an anesthetist experiencing acute vertigo while providing sedation to a patient undergoing a 3 Tesla MRI scan. After discussing previous reports, and the evidence surrounding MRI-induced vertigo, we review potential etiologies that include the effects of both static and time-varying magnetic fields on the vestibular apparatus. We conclude our review by discussing the occupational standards that exist for MRI exposure and methods to minimize the risks of MRI-induced vertigo for clinicians working in the MRI environment.

  12. Estigmas e representações sociais: desafios para a interação entre professores e alunos com Síndrome de Down

    Directory of Open Access Journals (Sweden)

    Geovane Tavares dos Santos

    2012-12-01

    Full Text Available Esse trabalho tem como fundamento a teoria sócio histórica, que considera o desenvolvimento psicológico individual a partir das interações sociais responsáveis por promover a construção do conhecimento de qualquer criança. A Escola a partir de suas atividades práticas, segundo Vygotsky (2003, p.33, é o local ideal para promover essa interação. Dentro desse ambiente, as interações entre professores e alunos são responsáveis por contribuir para o processo de ensino e aprendizagem. Porém, com base no fundamento da teoria sócio histórica e pautada na teoria sociológica de Serge Moscovici acerca das representações sociais, e as discussões que permeiam os estigmas sociais, é que este estudo tem a seguinte indagação: Quais os estigmas dos professores, formados a partir das representações sociais, e qual a interferência dos mesmos na interação com o aluno que tem síndrome de Down? Com essa questão o estudo tem enquanto objetivos identificar e compreender os estigmas dos professores, formados a partir das representações sociais, analisando sua interferência nas atitudes e interação com o aluno que tem síndrome de Down. Nesse estudo participaram 05 professores que trabalhavam com alunos com síndrome de Down, onde responderam a uma entrevista semiestruturada, que posteriormente foi transcrita e analisada. Os resultados corroboram que as representações sociais dos professores estão dotadas de estigmas negativos, pois os alunos com síndrome de Down no processo de ensino-aprendizagem são visto como indivíduos incapazes de aprender e se relacionar, tendo em vista que ainda prevalece o mito de que as pessoas com síndrome de Down são anormais.

  13. Complex cystic renal masses: Comparison of cyst complexity and Bosniak classification between 1.5 T and 3 T MRI

    Energy Technology Data Exchange (ETDEWEB)

    Rosenkrantz, Andrew B., E-mail: Andrew.Rosenkrantz@nyumc.org [Department of Radiology, NYU Langone Medical Center, 660 First Avenue, New York, NY 10016 (United States); Wehrli, Natasha E., E-mail: Natasha.Wehrli@nyumc.org [Department of Radiology, NYU Langone Medical Center, 660 First Avenue, New York, NY 10016 (United States); Mussi, Thais C., E-mail: thaiscaldara@gmail.com [Department of Radiology, NYU Langone Medical Center, 660 First Avenue, New York, NY 10016 (United States); Taneja, Samir S., E-mail: Samir.Taneja@nyumc.org [Department of Urology, Division of Urologic Oncology, NYU Langone Medical Center, 150 East 32nd Street, Suite 200, New York, NY 10016 (United States); Triolo, Michael J., E-mail: Michael.Triolo@nyumc.org [Department of Radiology, NYU Langone Medical Center, 660 First Avenue, New York, NY 10016 (United States)

    2014-03-15

    Purpose: To retrospectively compare perceived complexity and Bosniak cyst classification of cystic renal lesions between 1.5 T and 3 T MRI. Methods: 33 cystic renal lesions in 26 patients that underwent contrast-enhanced MRI at both 1.5 T and 3 T within a 12 month span were included. Two radiologists (R1, R2) independently assessed lesions, unaware of field strength, in terms of number of septations, septal thickening, mural thickening, presence of mural nodule, and Bosniak cyst category. Scores were compared between field strengths for each lesion. Results: R1 observed increases in septal number, septal thickening, mural thickening, and presence of mural nodule at 3 T in 8, 7, 4, and 2 lesions, and at 1.5 T in 3, 3, 2, and 0 lesions, respectively; R2 observed increases in septal number, septal thickening, mural thickening, and presence of mural nodule at 3 T in 3, 4, 3, and 0 lesions, and at 1.5 T in 2, 0, 0, and 0 lesions, respectively. R1 provided higher Bosniak category at 3 T in 9 cases and at 1.5 T in 4 cases; R2 provided higher Bosniak category at 3 T in 4 cases and at 1.5 T in 0 cases. Higher scores at 3 T than 1.5 T were associated with differences in advised clinical management in 7/9 cases for R1 and 4/4 cases for R2. Conclusion: There was an overall tendency for both readers to upgrade cyst complexity and Bosniak cyst category at 3 T than 1.5 T, which impacted advised management. Thus, we suggest that serial MRI evaluation of cystic renal lesions be performed at constant field strength.

  14. The putative auxin efflux carrier OsPIN3t is involved in the drought stress response and drought tolerance.

    Science.gov (United States)

    Zhang, Qian; Li, Jingjing; Zhang, Wenjiao; Yan, Shuning; Wang, Rui; Zhao, Junfeng; Li, Yujing; Qi, Zhiguang; Sun, Zongxiu; Zhu, Zhengge

    2012-12-01

    The phytohormone auxin plays a critical role in plant growth and development, and its spatial distribution largely depends on the polar localization of the PIN-FORMED (PIN) auxin efflux carrier family members. In this study, we identify a putative auxin efflux carrier gene in rice, OsPIN3t, which acts in auxin polar transport but is also involved in the drought stress response in rice. We show that OsPIN3t-GFP fusion proteins are localized in plasma membranes, and this subcellular localization changes under 1-N-naphthylphthalamic acid (NPA) treatment. The tissue-specific expression patterns of OsPIN3t were also investigated using a β-glucuronidase (GUS) reporter, which showed that OsPIN3t was mainly expressed in vascular tissue. The GUS activity in OsPIN3tpro::GUS plants increased by NAA treatment and decreased by NPA treatment. Moreover, knockdown of OsPIN3t caused crown root abnormalities in the seedling stage that could be phenocopied by treatment of wild-type plants with NPA, which indicated that OsPIN3t is involved in the control of polar auxin transport. Overexpression of OsPIN3t led to improved drought tolerance, and GUS activity significantly increased when OsPIN3tpro::GUS plants were subjected to 20% polyethylene glycol stress. Taken together, these results suggest that OsPIN3t is involved in auxin transport and the drought stress response, which suggests that a polar auxin transport pathway is involved in the regulation of the response to water stress in plants. © 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.

  15. T cell antigen receptor expression by subsets of Ly-2-L3T4- (CD8-CD4-) thymocytes

    DEFF Research Database (Denmark)

    Wilson, A; Ewing, T; Owens, T

    1988-01-01

    The V beta 8-specific mAb F23.1 and KJ16 were used as fluorescent stains to test for TCR expression on the surface of subpopulations of early, CD4-CD8- (L3T4-Ly-2-) thymocytes from adult CBA mice. A surprisingly high proportion (27%) of Ly-2-L3T4- thymocytes were strongly F23.1 and KJ16 positive....

  16. Suppressed intrinsic catalytic activity of GLUT1 glucose transporters in insulin-sensitive 3T3-L1 adipocytes

    Energy Technology Data Exchange (ETDEWEB)

    Harrison, S.A.; Buxton, J.M.; Czech, M.P. (Univ. of Massachusetts Medical Center, Worcester (United States))

    1991-09-01

    Previous studies indicated that the erythroid-type (GLUT1) glucose transporter isoform contributes to basal but not insulin-stimulated hexose transport in mouse 3T3-L1 adipocytes. In the present studies it was found that basal hexose uptake in 3T3-L1 adipocytes was about 50% lower than that in 3T3-L1 or CHO-K1 fibroblasts. Intrinsic catalytic activities of GLUT1 transporters in CHO-K1 and 3T3-L1 cells were compared by normalizing these hexose transport rates to GLUT1 content on the cell surface, as measured by two independent methods. Cell surface GLUT1 levels in 3T3-L1 fibroblasts and adipocytes were about 10- and 25-fold higher, respectively, than in CHO-K1 fibroblasts, as assessed with an anti-GLUT1 exofacial domain antiserum, delta. The large excess of cell surface GLUT1 transporters in 3T3-L1 adipocytes relative to CHO-K1 fibroblasts was confirmed by GLUT1 protein immunoblot analysis and by photoaffinity labeling (with 3-({sup 125}I)iodo-4-azidophenethylamido-7-O-succinyldeacetylforskolin) of glucose transporters in isolated plasma membranes. Thus, GLUT1 intrinsic activity is markedly reduced in 3T3-L1 fibroblasts compared with the CHO-K1 fibroblasts, and further reduction occurs upon differentiation to adipocytes. The authors conclude that a mechanism that markedly suppresses basal hexose transport catalyzed by GLUT1 is a major contributor to the dramatic insulin sensitivity of glucose uptake in 3T3-L1 adipocytes.

  17. Coculture with BJ fibroblast cells inhibits the adipogenesis and lipogenesis in 3T3-L1 cells

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Hyun Jeong [Department of Biochemistry, Kwandong University College of Medicine, Gangneung, Gangwondo 210-701 (Korea, Republic of); Park, Sahng Wook [Department of Biochemistry and Molecular Biology, Center for Chronic Metabolic Disease Research, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752 (Korea, Republic of); Kim, Hojeong [Department of Anatomy, Kwandong University College of Medicine, Gangneung, Gangwondo 210-701 (Korea, Republic of); Park, Sang-Kyu, E-mail: 49park@kd.ac.kr [Department of Biochemistry, Kwandong University College of Medicine, Gangneung, Gangwondo 210-701 (Korea, Republic of); Yoon, Dojun, E-mail: mozart@kd.ac.kr [Department of Biochemistry, Kwandong University College of Medicine, Gangneung, Gangwondo 210-701 (Korea, Republic of)

    2010-02-19

    Mouse or human fibroblasts are commonly used as feeder cells to prevent differentiation in stem or primary cell culture. In the present study, we addressed whether fibroblasts can affect the differentiation of adipocytes. We found that the differentiation of 3T3-L1 preadipocytes was strongly suppressed when the cells were cocultured with human fibroblast (BJ) cells. BrdU incorporation analysis indicated that mitotic clonal expansion, an early event required for 3T3-L1 cell adipogenesis, was not affected by BJ cells. The 3T3-L1 cell expression levels of peroxisome proliferator-activated receptor {gamma}2, CCAAT/enhancer-binding protein alpha (C/EBP{alpha}), sterol regulatory element binding protein-1c, and Krueppel-like factor 15, but not those of C/EBP{beta} or C/EBP{delta}, were decreased by coculture with BJ cells. When mature 3T3-L1 adipocytes were cocultured with BJ cells, their lipid contents were significantly reduced, with decreased fatty acid synthase expression and increased phosphorylated form of acetyl-CoA carboxylase 1. Our data indicate that coculture with BJ fibroblast cells inhibits the adipogenesis of 3T3-L1 preadipocytes and decreases the lipogenesis of mature 3T3-L1 adipocytes.

  18. Berberine reduces the expression of adipogenic enzymes and inflammatory molecules of 3T3-L1 adipocyte.

    Science.gov (United States)

    Choi, Bong-Hyuk; Ahn, In-Sook; Kim, Yu-Hee; Park, Ji-Won; Lee, So-Young; Hyun, Chang-Kee; Do, Myoung-Sool

    2006-12-31

    Berberine (BBR), an isoquinoline alkaloid, has a wide range of pharmacological effects, yet its exact mechanism is unknown. In order to understand the anti-adipogenic effect of BBR, we studied the change of expression of several adipogenic enzymes of 3T3-L1 cells by BBR treatment. First, we measured the change of leptin and glycerol in the medium of 3T3-L1 cells treated with 1 micrometer, 5 micrometer and 10 micrometer concentrations of BBR. We also measured the changes of adipogenic and lipolytic factors of 3T3-L1. In 3T3-L1 cells, both leptin and adipogenic factors (SREBP-1c, C/EBP-alpha, PPAR-gamma, fatty acid synthase, acetyl-CoA carboxylase, acyl-CoA synthase and lipoprotein lipase) were reduced by BBR treatment. Glycerol secretion was increased, whereas expression of lipolytic enzymes (hormone-sensitive lipase and perilipin) mRNA was slightly decreased. Next, we measured the change of inflammation markers of 3T3-L1 cells by BBR treatment. This resulted in the down-regulation of mRNA level of inflammation markers such as TNF-alpha, IL-6, C- reactive protein and haptoglobin. Taken together, our data shows that BBR has both anti-adipogenic and anti-inflammatory effects on 3T3-L1 adipocytes, and the anti-adipogenic effect seems to be due to the down-regulation of adipogenic enzymes and transcription factors.

  19. NIH 3T3 cells malignantly transformed by mot—2 show inactivation and cytoplasmic sequestration of the p53 protein

    Institute of Scientific and Technical Information of China (English)

    WADHWA; SYUICHITAKANO; 等

    1999-01-01

    In previous studies we have reported that a high level of expression of mot-2 protein results in malignant transformation of NIH 3T3 cells as analyzed by anchorage independent growth and nude mice assays [Kaul et al.,Oncogene,17,907-11,1998].Mot-2 was found to interact with tumor suppressor protein p53.The transient overexpression of mot-2 was inhibitory to transcriptional activation function of p53 [Wadhwa et al.,J.Biol.Chem.,273,2958691,1998].We demonstrate here that mot-2 transfected stable clone of NIH 3T3 that showed malignant properties indeed show inactivation of p53 function as assayed by exogenous p53 dependent reporter.The expression level of p53 in response to UV-irradiation was lower in NIH 3T3/mot-2 as compared to NIH 3T3 cells and also exhibited delay in reachingpeak.Furthermore,upon serum starvation p53 was seen to translocate to the nucleus in NIH 3T3,but not in its mot-3 derivative.The data suggests that mot-2 mediated cytoplasmic sequestration and inactivation of p53 may operate,at least in part,for malignant phenotype of NIH 3T3/mot-2 cells.

  20. T1rho MRI of menisci and cartilage in patients with osteoarthritis at 3T

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Ligong, E-mail: ligong.wang@nyumc.org [Quantitative Multinuclear Musculoskeletal Imaging Group (QMMIG), Center for Biomedical Imaging, Department of Radiology, New York University Langone Medical Center, New York, NY (United States); Chang, Gregory, E-mail: gregory.chang@nyumc.org [Quantitative Multinuclear Musculoskeletal Imaging Group (QMMIG), Center for Biomedical Imaging, Department of Radiology, New York University Langone Medical Center, New York, NY (United States); Xu, Jian, E-mail: jian.xu.sz@siemens.com [Siemens HealthCare, New York, NY (United States); Vieira, Renata L.R., E-mail: Renata.Vieira@nyumc.org [Quantitative Multinuclear Musculoskeletal Imaging Group (QMMIG), Center for Biomedical Imaging, Department of Radiology, New York University Langone Medical Center, New York, NY (United States); Krasnokutsky, Svetlana, E-mail: Svetlana.Krasnokutsky@nyumc.org [Division of Rheumatology, New York University Langone Medical Center, New York, NY (United States); Abramson, Steven, E-mail: StevenB.Abramson@nyumc.org [Division of Rheumatology, New York University Langone Medical Center, New York, NY (United States); Regatte, Ravinder R., E-mail: Ravinder.Regatte@nyumc.org [Quantitative Multinuclear Musculoskeletal Imaging Group (QMMIG), Center for Biomedical Imaging, Department of Radiology, New York University Langone Medical Center, New York, NY (United States)

    2012-09-15

    Objective: To assess and compare subregional and whole T1rho values (median ± interquartile range) of femorotibial cartilage and menisci in patients with doubtful (Kellgren–Lawrence (KL) grade 1) to severe (KL4) osteoarthritis (OA) at 3T. Materials and methods: 30 subjects with varying degrees of OA (KL1–4, 13 females, 17 males, mean age ± SD = 63.9 ± 13.1 years) were evaluated on a 3T MR scanner using a spin-lock-based 3D GRE sequence for T1rho mapping. Clinical proton density (PD)-weighted fast spin echo (FSE) images in sagittal (without fat saturation), axial, and coronal (fat-saturated) planes were acquired for cartilage and meniscus Whole-organ MR imaging score (WORMS) grading. Wilcoxon rank sum test was performed to determine whether there were any statistically significant differences between subregional and whole T1rho values of femorotibial cartilage and menisci in subjects with doubtful to severe OA. Results: Lateral (72 ± 10 ms, median ± interquartile range) and medial (65 ± 10 ms) femoral anterior cartilage subregions in moderate–severe OA subjects had significantly higher T1rho values (P < 0.05) than cartilage subregions and whole femorotibial cartilage in doubtful–minimal OA subjects. There were statistically significant differences in meniscus T1rho values of the medial posterior subregion of subjects with moderate–severe OA and T1rho values of all subregions and the whole meniscus in subjects with doubtful–minimal OA. When evaluated based on WORMS, statistically significant differences were identified in T1rho values between the lateral femoral anterior cartilage subregion in patients with WORMS5–6 (advanced degeneration) and whole femorotibial cartilage and all cartilage subregions in patients with WORMS0–1 (normal). Conclusion: T1rho values are higher in specific meniscus and femorotibial cartilage subregions. These findings suggest that regional damage of both femorotibial hyaline cartilage and menisci may be associated with

  1. Real-time motion- and B0-correction for LASER-localized spiral-accelerated 3D-MRSI of the brain at 3T.

    Science.gov (United States)

    Bogner, Wolfgang; Hess, Aaron T; Gagoski, Borjan; Tisdall, M Dylan; van der Kouwe, Andre J W; Trattnig, Siegfried; Rosen, Bruce; Andronesi, Ovidiu C

    2014-03-01

    The full potential of magnetic resonance spectroscopic imaging (MRSI) is often limited by localization artifacts, motion-related artifacts, scanner instabilities, and long measurement times. Localized adiabatic selective refocusing (LASER) provides accurate B1-insensitive spatial excitation even at high magnetic fields. Spiral encoding accelerates MRSI acquisition, and thus, enables 3D-coverage without compromising spatial resolution. Real-time position- and shim/frequency-tracking using MR navigators correct motion- and scanner instability-related artifacts. Each of these three advanced MRI techniques provides superior MRSI data compared to commonly used methods. In this work, we integrated in a single pulse sequence these three promising approaches. Real-time correction of motion, shim, and frequency-drifts using volumetric dual-contrast echo planar imaging-based navigators were implemented in an MRSI sequence that uses low-power gradient modulated short-echo time LASER localization and time efficient spiral readouts, in order to provide fast and robust 3D-MRSI in the human brain at 3T. The proposed sequence was demonstrated to be insensitive to motion- and scanner drift-related degradations of MRSI data in both phantoms and volunteers. Motion and scanner drift artifacts were eliminated and excellent spectral quality was recovered in the presence of strong movement. Our results confirm the expected benefits of combining a spiral 3D-LASER-MRSI sequence with real-time correction. The new sequence provides accurate, fast, and robust 3D metabolic imaging of the human brain at 3T. This will further facilitate the use of 3D-MRSI for neuroscience and clinical applications.

  2. A interação e o processo de negociação em L2

    Directory of Open Access Journals (Sweden)

    Cláudia Eliana Bassi

    2004-01-01

    Full Text Available Este trabalho apresenta uma investigação sobre a interação e as negociações desencadeadas por atividades comunicativas em uma sala de aula de nível básico de francês como segunda língua (L2. A partir da teoria sociointeracionista (Vygotsky, 1998, os resultados obtidos demonstraram que o processo interacional incentiva a troca de informações entre os aprendizes, favorecendo a negociação, e, também, que os aprendizes utilizaram estratégias de mediação para realizar as suas negociações. Os aprendizes receberam input modificado e tiveram mais oportunidades para a produção de output compreensível. Este relato, portanto, mostra que, ao negociar para a compreensão e a produção de textos em L2, os aprendizes se comprometeram, tornando-se agentes responsáveis e, sobretudo, colaboradores do processo de ensino/aprendizagem da L2.This paper investigated the interaction and the negotiations generated by communicative activities proposed in a French as a second language (L2 basic class. Based on the socio-interactionist theory (Vygotsky, 1998, the results show that the interactional process encourages the exchange of information among learners, helping negotiation. Besides that, learners also use mediating strategies to do the negotiations. The learners receive modified input and have more opportunities to produce comprehensible output. This paper, therefore, shows that, to negotiate, to understand and to produce L2 texts, the learners committed themselves, becoming responsible agents, and, above all, collaborators in the L2 learning/teaching process.

  3. Interação entre salinidade e bioestimulante no crescimento inicial de pinhão-manso

    Directory of Open Access Journals (Sweden)

    Francisco de A. de Oliveira

    2015-03-01

    Full Text Available Objetivou-se avaliar a interação entre salinidade e o uso de bioestimulante sobre o crescimento inicial de pinhão-manso. O experimento foi desenvolvido nos meses de agosto a outubro de 2012, na Universidade Federal Rural do Semi-Árido, em Mossoró, RN, utilizando o delineamento inteiramente casualizado, em esquema fatorial 2 x 4. Os tratamentos foram obtidos da combinação de dois níveis de sais da água de irrigação (0,5 e 5,0 dS m-1 e quatro intervalos de aplicação de bioestimulante (I1 – Ausência; I2 - 7 dias; I3 - 14 dias; I4 – 21 dias. Utilizou-se o fitorregulador comercial Stimulate® 10X, na concentração padrão de 18 mL L-1 de solução e se avaliaram as seguintes variáveis: altura, diâmetro do caule, comprimento da raiz principal, número de folhas, área foliar, massa seca de folhas, massa seca de caule, massa seca de raiz, massa seca total e razão massa seca da parte aérea/massa seca de raiz. O estresse salino provocou redução em todas as variáveis avaliadas e inibiu o efeito do bioestimulante nos intervalos utilizados. O uso de Stimulate® 10X não inibiu o efeito deletério da salinidade da água de irrigação sobre o crescimento inicial do pinhão-manso.

  4. Induction of adipocyte differentiation by polybrominated diphenyl ethers (PBDEs in 3T3-L1 cells.

    Directory of Open Access Journals (Sweden)

    Emily W Y Tung

    Full Text Available Polybrominated diphenyl ethers (PBDEs are a class of brominated flame retardants that were extensively used in commercial products. PBDEs are ubiquitous environmental contaminants that are both lipophilic and bioaccumulative. Effects of PBDEs on adipogenesis were studied in the 3T3-L1 preadipocyte cell model in the presence and absence of a known adipogenic agent, dexamethasone (DEX. A PBDE mixture designed to mimic body burden of North Americans was tested, in addition to the technical mixture DE-71 and the individual congener BDE-47. The mixture, DE-71, and BDE-47 all induced adipocyte differentiation as assessed by markers for terminal differentiation [fatty acid binding protein 4 (aP2 and perilipin] and lipid accumulation. Characterization of the differentiation process in response to PBDEs indicated that adipogenesis induced by a minimally effective dose of DEX was enhanced by these PBDEs. Moreover, C/EBPα, PPARγ, and LXRα were induced late in the differentiation process. Taken together, these data indicate that adipocyte differentiation is induced by PBDEs; they act in the absence of glucocorticoid and enhance glucocorticoid-mediated adipogenesis.

  5. mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes.

    Science.gov (United States)

    Sadler, Jessica B A; Roccisana, Jennifer; Virolainen, Minttu; Bryant, Nia J; Gould, Gwyn W

    2015-01-01

    Insulin stimulates the delivery of glucose transporter-4 (GLUT4)-containing vesicles to the surface of adipocytes. Depletion of the Sec1/Munc18 protein mVps45 significantly abrogates insulin-stimulated glucose transport and GLUT4 translocation. Here we show that depletion of mVps45 selectively reduced expression of VAMPs 2 and 4, but not other VAMP isoforms. Although we did not observe direct interaction of mVps45 with any VAMP isoform; we found that the cognate binding partner of mVps45, Syntaxin 16 associates with VAMPs 2, 4, 7 and 8 in vitro. Co-immunoprecipitation experiments in 3T3-L1 adipocytes revealed an interaction between Syntaxin 16 and only VAMP4. We suggest GLUT4 trafficking is controlled by the coordinated expression of mVps45/Syntaxin 16/VAMP4, and that depletion of mVps45 regulates VAMP2 levels indirectly, perhaps via reduced trafficking into specialized subcellular compartments.

  6. Hsp90 chaperones PPARγ and regulates differentiation and survival of 3T3-L1 adipocytes.

    Science.gov (United States)

    Nguyen, M T; Csermely, P; Sőti, C

    2013-12-01

    Adipose tissue dysregulation has a major role in various human diseases. The peroxisome proliferator-activated receptor-γ (PPARγ) is a key regulator of adipocyte differentiation and function, as well as a target of insulin-sensitizing drugs. The Hsp90 chaperone stabilizes a diverse set of signaling 'client' proteins, thereby regulates various biological processes. Here we report a novel role for Hsp90 in controlling PPARγ stability and cellular differentiation. Specifically, we show that the Hsp90 inhibitors geldanamycin and novobiocin efficiently impede the differentiation of murine 3T3-L1 preadipocytes. Geldanamycin at higher concentrations also inhibits the survival of both developing and mature adipocytes, respectively. Further, Hsp90 inhibition disrupts an Hsp90-PPARγ complex, leads to the destabilization and proteasomal degradation of PPARγ, and inhibits the expression of PPARγ target genes, identifying PPARγ as an Hsp90 client. A similar destabilization of PPARγ and a halt of adipogenesis also occur in response to protein denaturing stresses caused by a single transient heat-shock or proteasome inhibition. Recovery from stress restores PPARγ stability and adipocyte differentiation. Thus, our findings reveal Hsp90 as a critical stress-responsive regulator of adipocyte biology and offer a potential therapeutic target in obesity and the metabolic syndrome.

  7. ENHANCEMENT OF NIH3T3 CELL PROLIFERATION BY EXPRESSING MACROPHAGE COLONY STIMULATING FACTOR IN NUCLEI

    Institute of Scientific and Technical Information of China (English)

    曹震宇; 吴克复; 李戈; 林永敏; 张斌; 郑国光

    2003-01-01

    Objective: To explore the effects of nuclear M-CSF on the process of tumorigenesis. Methods: Functional part of M-CSF cDNA was inserted into an eukaryotic expression plasmid pCMV/myc/nuc, which can add three NLS to the C-terminal of the expressed protein and direct the protein into the cell nuclei. The constructed plasmid was transferred into NIH3T3 cells and the cell clones were selected by G-418 selection. Cell clones stable expressing target protein were identified by RT-PCR, ABC immunohistochemistry assay and Western blot. Cell growth kinetics analyses through growth curves, cell doubling time, MTT test and anti-sense oligodeoxynucleotide (ASODN) inhibiting cell growth test were performed to identify cells proliferation potential. Results: The transfected cells showed elevated proliferation potential over the control cells. Conclusion: Abnormal appearance of M-CSF in nucleus could enhance cell proliferation, which suggests that cytokine isoforms within cell nucleus might play transcription factor-like role.

  8. Complete genome sequence of Veillonella parvula type strain (Te3T)

    Energy Technology Data Exchange (ETDEWEB)

    Gronow, Sabine [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Welnitz, Sabine [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Chen, Feng [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Saunders, Elizabeth H [Los Alamos National Laboratory (LANL); Brettin, Thomas S [ORNL; Han, Cliff [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Bruce, David [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Chang, Yun-Juan [ORNL; Jeffries, Cynthia [Oak Ridge National Laboratory (ORNL); Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Mavromatis, K [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Chen, Amy [U.S. Department of Energy, Joint Genome Institute; Palaniappan, Krishna [U.S. Department of Energy, Joint Genome Institute; Chain, Patrick S. G. [Lawrence Livermore National Laboratory (LLNL); Rohde, Manfred [HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany; Goker, Markus [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Bristow, James [U.S. Department of Energy, Joint Genome Institute; Eisen, Jonathan [U.S. Department of Energy, Joint Genome Institute; Markowitz, Victor [U.S. Department of Energy, Joint Genome Institute; Hugenholtz, Philip [U.S. Department of Energy, Joint Genome Institute; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Klenk, Hans-Peter [DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute

    2010-01-01

    Veillonella parvula (Veillon and Zuber 1898) Pr vot 1933 is the type species of the genus Veillonella in the family Veillonellaceae within the order Clostridiales. The species V. parvula is of interest because it is frequently isolated from dental plaque in the human oral cavity and can cause opportunistic infections. The species is strictly anaerobic and grows as small cocci which usually occur in pairs. Veillonellae are characterized by their unusual metabolism which is centered on the activity of the enzyme methylmalonyl-CoA decarboxylase. Strain Te3T, the type strain of the species, was isolated from the human intestinal tract. Here we describe the features of this organism, together with the complete genome sequence, and annotation. This is the first complete genome sequence of a member of the large clostridial family Veillonellaceae, and the 2,132,142 bp long single replicon genome with its 1859 protein-coding and 61 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project.

  9. Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma

    Directory of Open Access Journals (Sweden)

    Mara Giavina-Bianchi

    2015-01-01

    Full Text Available NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79, rarely in in situ melanoma (1/10 and not in benign nevi (0/20. Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. NY-ESO-1 expression was associated with increased primary tumor thickness (P=0.007 and inversely correlated with superficial spreading melanoma (P<0.02. NY-ESO-1 expression was also associated with reduced numbers and density of CD3+ tumor infiltrating lymphocytes (P=0.017. When NY-ESO-1 protein was expressed, CD3+ T cells were less diffusely infiltrating the tumor and were more often arranged in small clusters (P=0.010 or as isolated cells (P=0.002 than in large clusters of more than five lymphocytes. No correlation of NY-ESO-1 expression with gender, age, tumor site, ulceration, lymph node sentinel status, or survival was observed. NY-ESO-1 expression in melanoma was associated with tumor progression, including increased tumor thickness, and with reduced tumor infiltrating lymphocytes.

  10. Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

    Science.gov (United States)

    Rejmontová, Petra; Capáková, Zdenka; Mikušová, Nikola; Maráková, Nela; Kašpárková, Věra; Lehocký, Marián; Humpolíček, Petr

    2016-01-01

    Polyaniline shows great potential and promises wide application in the biomedical field thanks to its intrinsic conductivity and material properties, which closely resemble natural tissues. Surface properties are crucial, as these predetermine any interaction with biological fluids, proteins and cells. An advantage of polyaniline is the simple modification of its surface, e.g., by using various dopant acids. An investigation was made into the adhesion, proliferation and migration of mouse embryonic fibroblasts on pristine polyaniline films and films doped with sulfamic and phosphotungstic acids. In addition, polyaniline films supplemented with poly (2-acrylamido-2-methyl-1-propanesulfonic) acid at various ratios were tested. Results showed that the NIH/3T3 cell line was able to adhere, proliferate and migrate on the pristine polyaniline films as well as those films doped with sulfamic and phosphotungstic acids; thus, utilization of said forms in biomedicine appears promising. Nevertheless, incorporating poly (2-acrylamido-2-methyl-1-propanesulfonic) acid altered the surface properties of the polyaniline films and significantly affected cell behavior. In order to reveal the crucial factor influencing the surface/cell interaction, cell behavior is discussed in the context of the surface energy of individual samples. It was clearly demonstrated that the lesser the difference between the surface energy of the sample and cell, the more cyto-compatible the surface is. PMID:27649159

  11. IT3T/2 - Creating, managingand using distribution lists with Simba2

    CERN Document Server

    CERN. Geneva; Vitè, Davide F; Wagner, Andreas

    2005-01-01

    CERN Technical Training 2005: IT3T - IT Technical Training Tutorials (Summer 2005) The new Listbox service interface SIMBA2 is a tool browsed everyday by a big amount of Listbox users. The different views for owners or members of the lists and the settings saved by them is capital for the correct function of the list. In this presentation we will introduce the Listbox Platform and the web interfaces and services that support it. How to deal with external accounts, creation of mailing lists and their classification in Human Resources, External or Normal Lists. How to operate with lists in Bulk and the importance of the archiving permissions. A special part of the presentation will show the criteria that listbox is using for authentication of mails against mail bombing, worms and spammers. The different ways to subscribe and unsubscribe to mailing lists and what are the main differences with the old platform. We will also dedicate a high level technical part for those who are administrators of mailing lists and...

  12. Intracytoplasmic triglyceride accumulation produced by dexamethasone in adult rat hepatocytes cultivated on 3T3 cells.

    Science.gov (United States)

    Mendoza-Figueroa, T; Hernandez, A; De Lourdes Lopez, M; Kuri-Harcuch, W

    1988-11-30

    Glucocorticoids, such as hydrocortisone (HC) and dexamethasone (DEX), when administered to rats, induce lipid accumulation within hepatocytes (fatty liver). To investigate whether glucocorticoids can produce triglyceride (TG) accumulation as they do in vivo and the involved mechanisms, we have used primary cultures of rat hepatocytes which synthesized and secrete triglycerides into the culture medium. Hepatocytes cultivated on a feeder layer of lethally treated 3T3 cells were exposed for 2 weeks to micromolar concentrations of DEX. This glucocorticoid caused morphological alterations and cells accumulated lipid droplets in their cytoplasm; the TG content increased up to 6-fold in a concentration- and time-dependent manner. The removal of [14C]acetic or [14C]oleic acid from the culture medium was not altered in the cultures treated with 50 micrograms/ml DEX but the incorporation of [14C]acetic and [14C]oleic acid into TG in these cultures was about 13-fold and 60% higher than in non-treated cells, respectively. On the other hand, hepatocytes treated with 50 micrograms/ml DEX for 2 weeks showed a 16-fold decrease in TG release and 40% inhibition in protein export, whereas synthesis of total cellular proteins was not altered. Our results show that corticosteroids, such as DEX, caused lipid accumulation in liver cells through an increased synthesis and/or esterification of fatty acids, but mostly through a decrease in the secretion of TG.

  13. Cultivation of adult rat hepatocytes on 3T3 cells: expression of various liver differentiated functions.

    Science.gov (United States)

    Kuri-Harcuch, W; Mendoza-Figueroa, T

    1989-08-01

    Adult rat hepatocytes were maintained in culture for at least 1 month without losing the expression of their differentiated functions; they were cultured on lethally treated 3T3 fibroblasts inoculated at 35,000 cells/cm2 with medium containing 10-25 micrograms/ml hydrocortisone. Hepatocytes showed their typical morphology; they formed bile canaliculi, microvilli, and intercellular junctions with desmosomes and nexus; some formed structures that may resemble the perisinusoidal space of Disse. In addition, they showed DNA synthesis and expressed some liver-specific functions. They synthesized albumin and other proteins, which were exported to the culture medium. Like parenchymal liver cells in vivo, de novo fatty acid synthesis and esterification took place, and more than 80% of the lipids synthesized by the hepatocytes were secreted into the medium as triglycerides; they also showed cytochrome-P450 activity that was inducible with phenobarbital, suggesting that the hepatocytes have the capacity to metabolize drugs. These culture conditions allow the study of various hepatocyte differentiated functions, and they may provide the means to analyze the effect on liver of hormones, viruses and hepatotoxic chemicals and drugs; they may also indicate conditions adequate for serial growth of hepatocytes.

  14. Culture of proliferating and differentiating fat-storing cells in 3T3-conditioned medium.

    Science.gov (United States)

    Mendoza-Figueroa, T; Argüello, C; Kuri-Harcuch, W

    1988-01-01

    There is growing evidence suggesting that hepatic fat-storing cells (FSC) or Ito cells have an important function in vitamin A storage and metabolism and in the synthesis of connective tissue components in normal liver and during fibrogenesis. The purified FSC acquire a fibroblastic morphology and their vitamin A content decreases in culture. We cultivated cells under in vitro conditions that allowed the expression of FSC morphological and functional characteristics for 3-4 weeks of primary culture. Cells were isolated from rat liver by the collagenase-perfusion method without further purification and cultured with 3T3-conditioned medium, which seemed to stimulate the selective proliferation of the FSC. After 8-10 days, round and stellate cells grew actively from a few precursor cells in the primary culture and were not subcultivated; the stellate cells had the ability to become round and vice versa and were highly motile. The cells had intracytoplasmic lipid droplets, a well developed rough endoplasmic reticulum, Golgi complex, numerous vesicles filled with electron-dense material, and extracellular matrix (ECM) components on their surface. Both stellate and round cells showed the presence of desmin by immunofluorescence and vitamin A autofluorescence, but lacked peroxidase activity. The culture conditions we describe allowed the selective proliferation of cells with morphological and functional characteristics of the FSC in the normal liver, raising the possibility of studying FSC proliferation and differentiation.

  15. Induction of adipocyte differentiation by polybrominated diphenyl ethers (PBDEs) in 3T3-L1 cells.

    Science.gov (United States)

    Tung, Emily W Y; Boudreau, Adèle; Wade, Michael G; Atlas, Ella

    2014-01-01

    Polybrominated diphenyl ethers (PBDEs) are a class of brominated flame retardants that were extensively used in commercial products. PBDEs are ubiquitous environmental contaminants that are both lipophilic and bioaccumulative. Effects of PBDEs on adipogenesis were studied in the 3T3-L1 preadipocyte cell model in the presence and absence of a known adipogenic agent, dexamethasone (DEX). A PBDE mixture designed to mimic body burden of North Americans was tested, in addition to the technical mixture DE-71 and the individual congener BDE-47. The mixture, DE-71, and BDE-47 all induced adipocyte differentiation as assessed by markers for terminal differentiation [fatty acid binding protein 4 (aP2) and perilipin] and lipid accumulation. Characterization of the differentiation process in response to PBDEs indicated that adipogenesis induced by a minimally effective dose of DEX was enhanced by these PBDEs. Moreover, C/EBPα, PPARγ, and LXRα were induced late in the differentiation process. Taken together, these data indicate that adipocyte differentiation is induced by PBDEs; they act in the absence of glucocorticoid and enhance glucocorticoid-mediated adipogenesis.

  16. Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells.

    Science.gov (United States)

    Shin, Dong-Mi; Choi, Kyeong-Mi; Lee, Youn-Sun; Kim, Wonkyun; Shin, Kyong-Oh; Oh, Seikwan; Jung, Jae-Chul; Lee, Mi Kyeong; Lee, Yong-Moon; Hong, Jin Tae; Yun, Yeo-Pyo; Yoo, Hwan-Soo

    2014-06-01

    Echinacea purpurea has been shown to have anti-diabetic activities; for example, it activates peroxisome proliferator-activated receptor γ (PPARγ) and increases insulin-stimulated glucose uptake. Adipogenesis has been used to study the insulin signaling pathway and to screen anti-diabetic compounds. The present study was conducted to investigate the effects of an ethanol extract of E. purpurea (EEEP) and its constituents on the insulin-induced adipocyte differentiation of 3T3-L1 preadipocytes. When adipocyte differentiation was induced with insulin plus 3-isobutyl-1-methylxanthine and dexamethasone, the accumulation of lipid droplets and the cellular triglyceride content were significantly increased by EEEP. The expressions of PPARγ and C/EBPα in adipocytes treated with EEEP were gradually increased as compared with control cells. Fat accumulation and triglyceride content of adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly increased as compared with control cells. The expressions of PPARγ and C/EBPα in adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly higher than in control cells. These results suggest EEEP promotes the adipogenesis that is partially induced by insulin and that dodeca-2(E),4(E)-dienoic acid isobutylamide appears to be responsible for EEEP-enhanced adipocyte differentiation.

  17. Increased NY-ESO-1 expression and reduced infiltrating CD3+ T cells in cutaneous melanoma.

    Science.gov (United States)

    Giavina-Bianchi, Mara; Giavina-Bianchi, Pedro; Sotto, Mirian Nacagami; Muzikansky, Alona; Kalil, Jorge; Festa-Neto, Cyro; Duncan, Lyn M

    2015-01-01

    NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79), rarely in in situ melanoma (1/10) and not in benign nevi (0/20). Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. NY-ESO-1 expression was associated with increased primary tumor thickness (P = 0.007) and inversely correlated with superficial spreading melanoma (P ESO-1 expression was also associated with reduced numbers and density of CD3+ tumor infiltrating lymphocytes (P = 0.017). When NY-ESO-1 protein was expressed, CD3+ T cells were less diffusely infiltrating the tumor and were more often arranged in small clusters (P = 0.010) or as isolated cells (P = 0.002) than in large clusters of more than five lymphocytes. No correlation of NY-ESO-1 expression with gender, age, tumor site, ulceration, lymph node sentinel status, or survival was observed. NY-ESO-1 expression in melanoma was associated with tumor progression, including increased tumor thickness, and with reduced tumor infiltrating lymphocytes.

  18. Hierarchical polymeric scaffolds support the growth of MC3T3-E1 cells.

    Science.gov (United States)

    Akbarzadeh, Rosa; Minton, Joshua A; Janney, Cara S; Smith, Tyler A; James, Paul F; Yousefi, Azizeh-Mitra

    2015-02-01

    Tissue engineering makes use of the principles of biology and engineering to sustain 3D cell growth and promote tissue repair and/or regeneration. In this study, macro/microporous scaffold architectures have been developed using a hybrid solid freeform fabrication/thermally induced phase separation (TIPS) technique. Poly(lactic-co-glycolic acid) (PLGA) dissolved in 1,4-dioxane was used to generate a microporous matrix by the TIPS method. The 3D-bioplotting technique was used to fabricate 3D macroporous constructs made of polyethylene glycol (PEG). Embedding the PEG constructs inside the PLGA solution prior to the TIPS process and subsequent extraction of PEG following solvent removal (1,4-dioaxane) resulted in a macro/microporous structure. These hierarchical scaffolds with a bimodal pore size distribution (300 μm) contained orthogonally interconnected macro-channels generated by the extracted PEG. The diameter of the macro-channels was varied by tuning the dispensing parameters of the 3D bioplotter. The in vitro cell culture using murine MC3T3-E1 cell line for 21 days demonstrated that these scaffolds could provide a favorable environment to support cell adhesion and growth.

  19. Accuracy of 3 T MR angiography in vertebral artery stenosis and coincidence with other cerebrovascular stenoses

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Hyun Seok; Kim, Dong Ik; Kim, Dong Joon; Kim, Jinna; Kim, Eun Soo; Lee, Seung-Koo [Yonsei University College of Medicine, Department of Radiology, Seoul (Korea, Republic of)

    2010-10-15

    Ostium of vertebral artery (VA) is a common site of pseudostenosis on contrast-enhanced MR angiography (CE-MRA). The purpose of this study was to determine the diagnostic accuracy of CE-MRA at 3 T in the evaluation of ostial stenosis of VA and to find associated coincidental stenoses using logistic regression analysis. One hundred and thirty-five VA ostial regions from 72 patients who received CE-MRA of neck vessels, intracranial time of flight (TOF) MRA, and digital subtraction angiography (DSA) were retrospectively reviewed. The sensitivity and specificity of the CE-MRA in detection of ostial stenosis were calculated with reference standard of DSA. Ostial stenosis on MRA was correlated with coincidental lesions in intracranial and cervical arteries by logistic regression analysis. The sensitivity and specificity of the CE-MRA were 100% and 80.4% for detection of significant stenosis. In case of significant stenoses, CE-MRA showed a tendency of overestimation with a false-positive rate of 52.5%. Logistic regression analysis showed that the stenoses of middle cerebral artery (MCA) on TOF MRA was associated with significant stenoses of VA ostia (OR = 5.84, 95% confidence intervals 1.41-24.17). CE-MRA is sensitive in detection of VA ostial stenosis although it has high false-positive rate. True positive ostial stenosis should be considered in cases of coincidental stenoses of MCA on TOF MRA. (orig.)

  20. Modulation of Osteogenesis in MC3T3-E1 Cells by Different Frequency Electrical Stimulation.

    Directory of Open Access Journals (Sweden)

    Yu Wang

    Full Text Available Electrical stimulation (ES is therapeutic to many bone diseases, from promoting fracture regeneration to orthopedic intervention. The application of ES offers substantial therapeutic potential, while optimal ES parameters and the underlying mechanisms responsible for the positive clinical impact are poorly understood. In this study, we assembled an ES cell culture and monitoring device. Mc-3T3-E1 cells were subjected to different frequency to investigate the effect of osteogenesis. Cell proliferation, DNA synthesis, the mRNA levels of osteosis-related genes, the activity of alkaline phosphatase (ALP, and intracellular concentration of Ca2+ were thoroughly evaluated. We found that 100 Hz could up-regulate the mRNA levels of collagen I, collagen II and Runx2. On the contrary, ES could down-regulate the mRNA levels of osteopontin (OPN. ALP activity assay and Fast Blue RR salt stain showed that 100 Hz could accelerate cells differentiation. Compared to the control group, 100 Hz could promote cell proliferation. Furthermore, 1 Hz to 10 Hz could improve calcium deposition in the intracellular matrix. Overall, these results indicate that 100Hz ES exhibits superior potentialities in osteogenesis, which should be beneficial for the clinical applications of ES for the treatment of bone diseases.

  1. Chemical Doping Effect on the Thermoelectric Properties of T Ga 3 (T = Fe, Ru, Os)

    Science.gov (United States)

    Haldolaarachchige, Neel; Karki, Amar; Phelan, Adam; Xiong, Yimin; Jin, Rongying; Chan, Julia; Stadler, Shane; Young, David

    2011-03-01

    Thermoelectric properties of chemically-doped intermetallic narrow-band semiconductors: T Ga 3 (T = Fe, Ru, Os) are reported. The parent compounds show semiconductor-like behavior (Eg ~ 0.2 eV, n290 K ~ 1018 cm3) with large n - type Seebeck coefficients at room temperature (S290 K ~ -300 μ V/K). The semiconductor-like FeGa 3 becomes metallic upon chemical doping (adding electron carriers), but RuGa 3 and OsGa 3 remain semiconducting. While the electrical resistivity and the Seebeck coefficients of all the compounds decrease with electron doping, the Seebeck coefficients remain fairly large and n - type, which leads to larger power factors than those of the pure samples. The thermal conductivity (κ 290K = 1.6 W/m K) of electron-doped FeGa 3 decreases, which increases the room temperature power factor by a large percentage (S2 / ρ 290K = 60 μ W/m K2) over that of pure FeGa 3 . This improvement in the power factor leads to a corresponding enhancement in the thermoelectric figure of merit (ZT) -- a factor of 5 increases above undoped polycrystalline FeGa 3 and two orders of magnitude improvement over that of pure single crystalline FeGa 3 .

  2. Fisetin induces Sirt1 expression while inhibiting early adipogenesis in 3T3-L1 cells.

    Science.gov (United States)

    Kim, Sang Chon; Kim, Yoo Hoon; Son, Sung Wook; Moon, Eun-Yi; Pyo, Suhkneung; Um, Sung Hee

    2015-11-27

    Fisetin (3,7,3',4'-tetrahydroxyflavone) is a naturally found flavonol in many fruits and vegetables and is known to have anti-aging, anti-cancer and anti-viral effects. However, the effects of fisetin on early adipocyte differentiation and the epigenetic regulator controlling adipogenic transcription factors remain unclear. Here, we show that fisetin inhibits lipid accumulation and suppresses the expression of PPARγ in 3T3-L1 cells. Fisetin suppressed early stages of preadipocyte differentiation, and induced expression of Sirt1. Depletion of Sirt1 abolished the inhibitory effects of fisetin on intracellular lipid accumulation and on PPARγ expression. Mechanistically, fisetin facilitated Sirt1-mediated deacetylation of PPARγ and FoxO1, and enhanced the association of Sirt1 with the PPARγ promoter, leading to suppression of PPARγ transcriptional activity, thereby repressing adipogenesis. Lowering Sirt1 levels reversed the effects of fisetin on deacetylation of PPARγ and increased PPARγ transactivation. Collectively, our results suggest the effects of fisetin in increasing Sirt1 expression and in epigenetic control of early adipogenesis. Copyright © 2015 Elsevier Inc. All rights reserved.

  3. Withaferin A induces apoptosis and inhibits adipogenesis in 3T3-L1 adipocytes.

    Science.gov (United States)

    Park, Hea Jin; Rayalam, Srujana; Della-Fera, Mary Anne; Ambati, Suresh; Yang, Jeong-Yeh; Baile, Clifton A

    2008-01-01

    Withaferin A (WA), a highly oxygenated steroidal lactone that is found in the medicinal plant Withania somnifera (also called ashwagandha) has been reported to have anti-tumor, anti-angiogenesis, and pro-apoptotic activity. We investigated the effects of WA on viability, apoptosis and adipogenesis in 3T3-L1 adipocytes. Pre- and post-confluent preadipocytes and mature adipocytes were treated with WA (1-25 microM) up to 24 hrs. Viability and apoptosis were measured by CellTiter-Blue Cell Viability Assay and single strand DNA ELISA Assay, respectively. WA decreased viability and induced apoptosis in all stages of cells. Induction of apoptosis by WA in mature adipocytes was mediated by increased ERK1/2 phosphorylation and altered Bax and Bcl2 protein expression. The effect of WA on adipogenesis was examined by AdipoRed Assay after treating with WA (0.1-1 microM) during the differentiation period. WA decreased lipid accumulation in a dose-dependent manner and decreased the expression of peroxisome proliferator-activated receptor gamma, CCAAT/enhancer binding protein alpha and adipocyte fatty acid binding protein. The effects on apoptosis and lipid accumulation were also confirmed with Hoechst staining and Oil Red O staining, respectively. These results show that WA acts on adipocytes to reduce cell viability and adipogenesis and also induce apoptosis.

  4. Effects of leptin on apoptosis and adipogenesis in 3T3-L1 adipocytes.

    Science.gov (United States)

    Ambati, Suresh; Kim, Hye-Kyeong; Yang, Jeong-Yeh; Lin, Ji; Della-Fera, Mary Anne; Baile, Clifton A

    2007-02-01

    Leptin has been demonstrated to induce adipose tissue apoptosis, which can contribute to the decrease of adiposity, after either central nervous system or peripheral administration. However, it is not known whether leptin acts only centrally to initiate a signal or can also act directly on adipocytes to induce apoptosis. The objective of this study was to determine the direct effect of leptin on adipocyte apoptosis and adipogenesis in vitro using 3T3-L1 cell lines. An ELISA for single stranded DNA, which is highly specific for apoptotic cells, was used to quantify apoptosis. Preconfluent preadipocytes treated with 10(-9), 10(-8), 10(-7), and 10(-6)M leptin showed inhibitory effects on cell viability, and similar observations were also found in maturing preadipocytes treated during day 0-2 and day 2-4 of maturation. After 48 h incubation with 10(-6)M leptin, LDH release was increased by 24.3% (p<0.05) in preconfluent preadipocytes and by 108.5% (p<0.01) in maturing preadipocytes. However, ssDNA analysis revealed no increased apoptosis in preconfluent or maturing preadipocytes or in mature adipocytes treated with leptin. Leptin significantly reduced lipid accumulation and GPDH activity in maturing preadipocytes, demonstrating an inhibitory effect of leptin on adipogenesis. These results indicate that leptin does not act directly to induce adipocyte apoptosis, but can act directly to inhibit maturation of preadipocytes.

  5. Anti-obesity effects of xanthohumol plus guggulsterone in 3T3-L1 adipocytes.

    Science.gov (United States)

    Rayalam, Srujana; Yang, Jeong-Yeh; Della-Fera, Mary Anne; Park, Hea Jin; Ambati, Suresh; Baile, Clifton A

    2009-08-01

    Xanthohumol (XN) and guggulsterone (GS) have each been shown to inhibit adipogenesis and induce apoptosis in adipocytes. In the present study effects of the combination of XN + GS on 3T3-L1 adipocyte apoptosis and adipogenesis were investigated. Mature adipocytes were treated with XN and GS individually and in combination. XN and GS individually decreased cell viability, but XN + GS caused an enhanced decrease in viability and potentiated induction of apoptosis. Likewise, XN + GS caused a potentiated increase in caspase-3/7 activation, whereas neither of the compounds showed any effect individually. In addition, western blot analysis revealed that XN + GS increased Bax expression and decreased Bcl-2 expression, whereas individual compounds did not show any significant effect. XN and GS both decreased lipid accumulation. Individually, XN at 1.5 microM and GS at 3.12 microM decreased lipid accumulation by 26 +/- 4.5% (P < .001) each, whereas XN1.5 + GS3.12 decreased lipid accumulation by 78.2 +/- 1.8% (P < .001). Moreover, expression of the adipocyte-specific proteins was down-regulated with XN1.5 + GS3.12, but no effect was observed with the individual compounds. Finally, XN + GS caused an enhanced stimulation of lipolysis. Thus, combination of XN and GS is more potent in exerting anti-obesity effects than additive effects of the individual compounds.

  6. MRBrainS Challenge: Online Evaluation Framework for Brain Image Segmentation in 3T MRI Scans

    Directory of Open Access Journals (Sweden)

    Adriënne M. Mendrik

    2015-01-01

    Full Text Available Many methods have been proposed for tissue segmentation in brain MRI scans. The multitude of methods proposed complicates the choice of one method above others. We have therefore established the MRBrainS online evaluation framework for evaluating (semiautomatic algorithms that segment gray matter (GM, white matter (WM, and cerebrospinal fluid (CSF on 3T brain MRI scans of elderly subjects (65–80 y. Participants apply their algorithms to the provided data, after which their results are evaluated and ranked. Full manual segmentations of GM, WM, and CSF are available for all scans and used as the reference standard. Five datasets are provided for training and fifteen for testing. The evaluated methods are ranked based on their overall performance to segment GM, WM, and CSF and evaluated using three evaluation metrics (Dice, H95, and AVD and the results are published on the MRBrainS13 website. We present the results of eleven segmentation algorithms that participated in the MRBrainS13 challenge workshop at MICCAI, where the framework was launched, and three commonly used freeware packages: FreeSurfer, FSL, and SPM. The MRBrainS evaluation framework provides an objective and direct comparison of all evaluated algorithms and can aid in selecting the best performing method for the segmentation goal at hand.

  7. A 22-channel receive array with Helmholtz transmit coil for anesthetized macaque MRI at 3 T.

    Science.gov (United States)

    Janssens, Thomas; Keil, Boris; Serano, Peter; Mareyam, Azma; McNab, Jennifer A; Wald, Lawrence L; Vanduffel, Wim

    2013-11-01

    The macaque monkey is an important model for cognitive and sensory neuroscience that has been used extensively in behavioral, electrophysiological, molecular and, more recently, neuroimaging studies. However, macaque MRI has unique technical differences relative to human MRI, such as the geometry of highly parallel receive arrays, which must be addressed to optimize imaging performance. A 22-channel receive coil array was constructed specifically for rapid high-resolution anesthetized macaque monkey MRI at 3 T. A local Helmholtz transmit coil was used for excitation. Signal-to-noise ratios (SNRs) and noise amplification for parallel imaging were compared with those of single- and four-channel receive coils routinely used for macaque MRI. The 22-channel coil yielded significant improvements in SNR throughout the brain. Using this coil, the SNR in peripheral brain was 2.4 and 1.7 times greater than that obtained with single- or four-channel coils, respectively. In the central brain, the SNR gain was 1.5 times that of both the single- and four-channel coils. Finally, the performance of the array for functional, anatomical and diffusion-weighted imaging was evaluated. For all three modalities, the use of the 22-channel array allowed for high-resolution and accelerated image acquisition.

  8. MC3T3-E1 Cells on Titanium Surfaces with Nanometer Smoothness and Fibronectin Immobilization

    Directory of Open Access Journals (Sweden)

    Tohru Hayakawa

    2012-01-01

    Full Text Available The present study was aimed to evaluate the viability and total protein contents of osteoblast-like cells on the titanium surface with different surface mechanical treatment, namely, nanometer smoothing (Ra: approximately 2.0 nm and sandblasting (Ra: approximately 1.0 μm, and biochemical treatment, namely, with or without fibronectin immobilization. Fibronectin could be easily immobilized by tresyl chloride-activation technique. MC3T3-E1 cells were seeded on the different titanium surfaces. Cell viability was determined by MTT assay. At 1 day of cell culture, there were no significant differences in cell viability among four different titanium surfaces. At 11 days, sandblasted titanium surface with fibronectin immobilization showed the significantly highest cell viability than other titanium surface. No significant differences existed for total protein contents among four different titanium surfaces at 11 days of cell culture. Scanning electron microscopy observation revealed that smoothness of titanium surface produced more spread cell morphologies, but that fibronectin immobilization did not cause any changes of the morphologies of attached cells. Fibronectin immobilization provided greater amount of the number of attached cells and better arrangement of attached cells. In conclusion, the combination of sandblasting and fibronectin immobilization enhanced the cell viability and fibronectin immobilization providing better arrangements of attached cells.

  9. The role of Akt on Arsenic trioxide suppression of 3T3-L1 preadipocyte differentiation

    Institute of Scientific and Technical Information of China (English)

    Zhi Xin WANG; Chun Sun JIANG; Lei LIU; Xiao Hui WANG; Hai Jing JIN; Qiao WU; Quan CHEN

    2005-01-01

    The present study investigates the molecular details of how arsenic trioxide inhibits preadipocyte differentiation and examines the role of Akt/PKB in regulation of differentiation and apoptosis. Continual exposure of arsenic trioxide, at the clinic achievable dosage that does not induce apoptosis, suppressed 3T3-L1 cell differentiation into fat cells by inhibiting the expression of PPARγ and C/EBPα and disrupting the interaction between PPARγ and RXRα, which determines the programming of the adipogenic genes. Interestingly, if we treated the cells for 12 or 24 h and then withdrew arsenic trioxide, the cells were able to differentiate to the comparable levels of untreated cells as assayed by the activity of GAPDH, the biochemical marker of preadipocyte differentiation. Long term treatment blocked the differentiation and the activity of GAPDH could not recover to the comparable levels of untreated cells. Continual exposure of arsenic trioxide caused accumulation in G2/M phase and the accumulation of p21. We found that arsenic trioxide induced the expression and the phosphorylation of Akt/PKB and it inhibited the interaction between Akt/PKB and PPARγ. Akt/PKB inhibitor appears to block the arsenic trioxide suppression of differentiation. Our results suggested that Akt/PKB may play a role in suppression of apoptosis and negatively regulate preadipocyte differentiation.

  10. Effects of Methylmercury exposure in 3T3-L1 Adipocytes

    Directory of Open Access Journals (Sweden)

    Theresa Vertigan

    2017-02-01

    Full Text Available Mercury-containing compounds are environmental pollutants that have become increasingly consequential in the Arctic regions of North America due to processes of climate change increasing their release and availability at northern latitudes. Currently, the form of mercury known to be most detrimental to human health is methylmercury, CH3Hg+, which is found in the environment and accumulates in the tissues of piscivores, including those consumed by Alaska Natives through subsistence gathering. Much is known about the neurotoxicity of methylmercury after exposure to high concentrations, but little is known about toxicity to other tissues and cell types, particularly for long-term exposure and the lower concentrations that would occur through fish consumption. Effects of methylmercury exposure on 3T3-L1 adipocytes in culture were assessed using assays for cytotoxicity and an ELISA assay for vascular endothelial growth factor (VEGF, a signaling molecule shown to be important for maintaining metabolic status in adipose tissue. Results showed that exposure to methylmercury leads to significant toxicity in adipocytes at exposures of 100 ng/mL during later stages of differentiation, but lower methylmercury concentrations produced little to no toxicity. Results also showed that VEGF secretion is elevated in adipocytes exposed to methylmercury after the process of differentiating into mature, fat-storing cells. These results provide a basis for further exploration into metabolic consequences of methylmercury exposure on specific cell types and cell models.

  11. Hyperkalemic periodic paralysis and permanent weakness: 3-T MR imaging depicts intracellular 23Na overload--initial results.

    Science.gov (United States)

    Amarteifio, Erick; Nagel, Armin M; Weber, Marc-André; Jurkat-Rott, Karin; Lehmann-Horn, Frank

    2012-07-01

    To assess whether myoplasmic ionic sodium (Na+) is increased in muscles of patients with hyperkalemic periodic paralysis (HyperPP) with 3-T sodium 23 (23Na) magnetic resonance (MR) imaging and to evaluate the effect of medical treatment on sodium-induced muscle edema. This study received institutional review board approval; written informed consent was obtained. Proton (hydrogen 1 [1H]) and 23Na MR of both calves were performed in 12 patients with HyperPP (mean age, 48 years±14 [standard deviation]) and 12 healthy volunteers (mean age, 38 years±12) before and after provocation (unilateral cooling, one calf). 23Na MR included spin-density, T1-weighted, and inversion-recovery (IR) sequences. Total sodium concentration and normalized signal intensities (SIs) were evaluated within regions of interest (ROIs). Muscle strength was measured with the British Medical Research Council (MRC) grading scale. Five patients underwent follow-up MR after diuretic treatment. During rest, mean myoplasmic Na+ concentration was significantly higher in HyperPP with permanent weakness (40.7 μmol/g±3.9) compared with HyperPP with transient weakness (31.3 μmol/g±4.8) (P=.004). Mean SI in 23Na IR MR was significantly higher in HyperPP with permanent weakness (0.83±0.04; median MRC, grade 4; range, 3-5) compared with HyperPP without permanent weakness (0.67±0.05; median MRC, grade 5; range, 4-5) (P=.002). Provocation reduced muscle strength in HyperPP (before provocation, median MRC, 5; range, 3-5; after provocation, median MRC, 3; range, 1-4) and increased SI in 23Na IR from 0.75±0.09 to 0.86±0.10 (P=.004). Spin-density and T1-weighted sequences were less sensitive, particularly to cold-induced Na+ changes. 23Na IR SI remained unchanged in volunteers (0.53±0.06 before and 0.54±0.06 after provocation, P=.3). Therapy reduced mean SI in 23Na IR sequence from 0.85±0.04 to 0.64±0.11. 23Na MR imaging depicts increased myoplasmic Na+ in HyperPP with permanent weakness. Na+ overload

  12. Low eddy current RF shielding enclosure designs for 3T MR application