WorldWideScience

Sample records for 3p core levels

  1. A study of angle-resolved photoemission extended fine structure as applied to the Ni 3p, Cu 3s, and Cu 3p core levels of the respective clean (111) surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Huff, W.R.A.; Moler, E.J.; Kellar, S.A. [Lawrence Berkeley National Lab., CA (United States)] [and others

    1997-04-01

    The first non-s initial state angle-resolved photoemission extended fine structure (ARPEFS) study of clean surfaces for the purpose of further understanding the technique is reported. The surface structure sensitivity of ARPEFS applied to clean surfaces and to arbitrary initial states is studied using normal photoemission data taken from the Ni 3p core levels of a Ni(111) single crystal and the Cu 3s and the Cu 3p core-levels of a Cu(111) single crystal. The Fourier transforms of these clean surface data are dominated by backscattering. Unlike the s initial state data, the p initial state data show a peak in the Fourier transform corresponding to in-plane scattering from the six nearest-neighbors to the emitter. Evidence was seen for single-scattering events from in the same plane as the emitters and double-scattering events. Using a newly developed, multiple-scattering calculation program, ARPEFS data from clean surfaces and from p initial states can be modeled to high precision. Although there are many layers of emitters when measuring photoemission from a clean surface, test calculations show that the ARPEFS signal is dominated by photoemission from atoms in the first two crystal layers. Thus, ARPEFS applied to clean surfaces is sensitive to surface reconstruction. The known contraction of the first two Cu(111) layers is confirmed. The best-fit calculation for clean Ni(111) indicates an expansion of the first two layers. To better understand the ARPEFS technique, the authors studied s and non-s initial state photoemission from clean metal surfaces.

  2. Association of Serum MiR-142-3p and MiR-101-3p Levels with Acute Cellular Rejection after Heart Transplantation

    Science.gov (United States)

    Sukma Dewi, Ihdina; Hollander, Zsuzsanna; Lam, Karen K.; McManus, Janet-Wilson; Tebbutt, Scott J.; Ng, Raymond T.; Keown, Paul A.; McMaster, Robert W.; McManus, Bruce M.; Gidlöf, Olof; Öhman, Jenny

    2017-01-01

    Background Identifying non-invasive and reliable blood-derived biomarkers for early detection of acute cellular rejection in heart transplant recipients is of great importance in clinical practice. MicroRNAs are small molecules found to be stable in serum and their expression patterns reflect both physiological and underlying pathological conditions in human. Methods We compared a group of heart transplant recipients with histologically-verified acute cellular rejection (ACR, n = 26) with a control group of heart transplant recipients without allograft rejection (NR, n = 37) by assessing the levels of a select set of microRNAs in serum specimens. Results The levels of seven microRNAs, miR-142-3p, miR-101-3p, miR-424-5p, miR-27a-3p, miR-144-3p, miR-339-3p and miR-326 were significantly higher in ACR group compared to the control group and could discriminate between patients with and without allograft rejection. MiR-142-3p and miR-101-3p had the best diagnostic test performance among the microRNAs tested. Serum levels of miR-142-3p and miR-101-3p were independent of calcineurin inhibitor levels, as measured by tacrolimus and cyclosporin; kidney function, as measured by creatinine level, and general inflammation state, as measured by CRP level. Conclusion This study demonstrated two microRNAs, miR-142-3p and miR-101-3p, that could be relevant as non-invasive diagnostic tools for identifying heart transplant patients with acute cellular rejection. PMID:28125729

  3. BOREAS Level-3p Landsat TM Imagery: Geocoded and Scaled At-sensor Radiance

    Science.gov (United States)

    Nickeson, Jaime; Knapp, David; Newcomer, Jeffrey A.; Hall, Forrest G. (Editor); Cihlar, Josef

    2000-01-01

    For BOReal Ecosystem-Atmosphere Study (BOREAS), the level-3p Landsat Thematic Mapper (TM) data were used to supplement the level-3s Landsat TM products. Along with the other remotely sensed images, the Landsat TM images were collected in order to provide spatially extensive information over the primary study areas. This information includes radiant energy, detailed land cover, and biophysical parameter maps such as Fraction of Photosynthetically Active Radiation (FPAR) and Leaf Area Index (LAI). Although very similar to the level-3s Landsat TM products, the level-3p images were processed with ground control information, which improved the accuracy of the geographic coordinates provided. Geographically, the level-3p images cover the BOREAS Northern Study Area (NSA) and Southern Study Area (SSA). Temporally, the four images cover the period of 20-Aug-1988 to 07-Jun-1994. Except for the 07-Jun-1994 image, which contains seven bands, the other three contain only three bands.

  4. Reduced miR-659-3p levels correlate with progranulin increase in hypoxic conditions: implications for frontotemporal dementia.

    Directory of Open Access Journals (Sweden)

    Paola ePiscopo

    2016-05-01

    Full Text Available Progranulin (PGRN is a secreted protein expressed ubiquitously throughout the body, including the brain, where it localizes in neurons and activated microglia. Loss-of-function mutations in the GRN gene are an important cause of familial Frontotemporal Lobar Degeneration (FTLD. PGRN has a neurotrophic and anti-inflammatory activity, and it is neuroprotective in several injury conditions, such as oxygen or glucose deprivation, oxidative injury, and hypoxic stress. Indeed, we have previously demonstrated that hypoxia induces the up-regulation of GRN transcripts. Several studies have shown microRNAs involvement in hypoxia. Moreover, in FTLD patients with a genetic variant of GRN (rs5848, the reinforcement of miR-659-3p binding site has been suggested to be a risk factor. Here, we report that miR-659-3p interacts directly with GRN 3’UTR as shown by luciferase assay in HeLa cells and ELISA and Western Blot analysis in HeLa and Kelly cells. Moreover, we demonstrate the physical binding between GRN mRNA and miR-659-3p employing a miRNA capture-affinity technology in SK-N-BE and Kelly cells. In order to study miRNAs involvement in hypoxia-mediated up-regulation of GRN, we evaluated miR-659-3p levels in SK-N-BE cells after 24h of hypoxic treatment, finding them inversely correlated to GRN transcripts. Furthermore, we analyzed an animal model of asphyxia, finding that GRN mRNA levels increased at post-natal day (pnd 1 and pnd 4 in rat cortices subjected to asphyxia in comparison to control rats and miR-659-3p decreased at pnd 4 just when GRN reached the highest levels. Our results demonstrate the interaction between miR-659-3p and GRN transcript and the involvement of miR-659-3p in GRN up-regulation mediated by hypoxic/ischemic insults.

  5. Trends in adsorbate induced core level shifts

    Science.gov (United States)

    Nilsson, Viktor; Van den Bossche, Maxime; Hellman, Anders; Grönbeck, Henrik

    2015-10-01

    Photoelectron core level spectroscopy is commonly used to monitor atomic and molecular adsorption on metal surfaces. As changes in the electron binding energies are convoluted measures with different origins, calculations are often used to facilitate the decoding of experimental signatures. The interpretation could in this sense benefit from knowledge on trends in surface core level shifts for different metals and adsorbates. Here, density functional theory calculations have been used to systematically evaluate core level shifts for (111) and (100) surfaces of 3d, 4d, and 5d transition metals upon CO, H, O and S adsorption. The results reveal trends and several non-intuitive cases. Moreover, the difficulties correlating core level shifts with charging and d-band shifts are underlined.

  6. Core level shifts of intercalated graphene

    Science.gov (United States)

    Schröder, Ulrike A.; Petrović, Marin; Gerber, Timm; Martínez-Galera, Antonio J.; Grånäs, Elin; Arman, Mohammad A.; Herbig, Charlotte; Schnadt, Joachim; Kralj, Marko; Knudsen, Jan; Michely, Thomas

    2017-03-01

    Through intercalation of metals and gases the Dirac cone of graphene on Ir(111) can be shifted with respect to the Fermi level without becoming destroyed by strong hybridization. Here, we use x-ray photoelectron spectroscopy to measure the C 1s core level shift (CLS) of graphene in contact with a number of structurally well-defined intercalation layers (O, H, Eu, and Cs). By analysis of our own and additional literature data for decoupled graphene, the C 1s CLS is found to be a non-monotonic function of the doping level. For small doping levels the shifts are well described by a rigid band model. However, at larger doping levels, a second effect comes into play which is proportional to the transferred charge and counteracts the rigid band shift. Moreover, not only the position, but also the C 1s peak shape displays a unique evolution as a function of doping level. Our conclusions are supported by intercalation experiments with Li, with which, due to the absence of phase separation, the doping level of graphene can be continuously tuned.

  7. Photoexcitation and photoionization from the 2p{sup 5}3p[5/2]{sub 2,3} levels in neon

    Energy Technology Data Exchange (ETDEWEB)

    Baig, M. A.; Bokhari, I. A.; Rafiq, M.; Kalyar, M. A.; Hussian, T.; Ali, Raheel; Piracha, N. K. [Atomic and Molecular Physics Laboratory, Department of Physics, Quaid-i-Azam University, 45320 Islamabad (Pakistan); Department of Physics, John Carroll University, 20700 N. Park Boulevard, University Heights, Ohio 44118 (United States)

    2011-07-15

    We present measurements of the excitation spectra from the 2p{sup 5}3p[5/2]{sub 3,2} levels in neon using two-step laser excitation and ionization in conjunction with an optogalvanic detection in dc and rf discharge cells. The 2p{sup 5}3p[5/2]{sub 3,2} intermediate levels have been approached via the collisionally populated 2p{sup 5}3s[3/2]{sub 2} metastable level. The Rydberg series 2p{sup 5}({sup 2}P{sub 3/2})nd[7/2]{sub 4} (12 {<=}n{<=} 44), 2p{sup 5}({sup 2}P{sub 3/2})ns[3/2]{sub 2} (13 {<=}n{<=} 35) and the parity forbidden transitions 2p{sup 5}({sup 2}P{sub 3/2})np[5/2]{sub 3} (13 {<=}n{<=} 19) have been observed from the 2p{sup 5}3p[5/2]{sub 3} level, whereas the 2p{sup 5}({sup 2}P{sub 3/2})nd[7/2]{sub 3} (12 {<=}n{<=} 44), 2p{sup 5}({sup 2}P{sub 3/2})ns[3/2]{sub 2} (13 {<=}n{<=} 35), and 2p{sup 5}({sup 2}P{sub 1/2})nd'[5/2]{sub 3} (9 {<=}n{<=} 12) Rydberg series have been observed from the 2p{sup 5}3p[5/2]{sub 2} level in accordance with the {Delta}J ={Delta}K = {+-} 1 selection rules. The photoionization cross sections from the 2p{sup 5}3p[5/2]{sub 3} intermediate level have been measured at eight ionizing laser wavelengths (399, 395, 390, 385, 380, 370, 364, and 355 nm) and that from the 2p{sup 5}3p[5/2]{sub 2} level at 401.8 nm. These measurements are in excellent agreement with the experimental values reported in the literature, while the experimental data lie much below the theoretically calculated photoionization cross sections curve.

  8. Laser techniques for spectroscopy of core-excited atomic levels

    Science.gov (United States)

    Harris, S. E.; Young, J. F.; Falcone, R. W.; Rothenberg, J. E.; Willison, J. R.

    1982-01-01

    We discuss three techniques which allow the use of tunable lasers for high resolution and picosecond time scale spectroscopy of core-excited atomic levels. These are: anti-Stokes absorption spectroscopy, laser induced emission from metastable levels, and laser designation of selected core-excited levels.

  9. Association of Circulating MicroRNA-124-3p Levels With Outcomes After Out-of-Hospital Cardiac Arrest

    DEFF Research Database (Denmark)

    Devaux, Yvan; Dankiewicz, Josef; Salgado-Somoza, Antonio

    2016-01-01

    and survival of patients with cardiac arrest after targeted temperature management at 33°C or 36°C. Five hundred seventy-nine patients who survived the first 24 hours after the return of spontaneous circulation and who had blood samples available for miRNA assessment were enrolled from 29 intensive care units...... in 9 countries from November 11, 2010, to January 10, 2013. Final follow-up was completed on July 3, 2013, and data were assessed from February 1, 2014, to February 1, 2016. INTERVENTIONS: Blood sampling at 48 hours after the return of spontaneous circulation. MAIN OUTCOMES AND MEASURES: The primary...... end point was poor neurologic outcome at 6 months (cerebral performance category score, 3 [severe neurologic sequelae], 4 [coma], or 5 [death]). The secondary end point was survival until the end of the trial. Circulating levels of miRNAs were measured by sequencing and polymerase chain reaction...

  10. Core level photoemission of rotaxanes : A summary on binding energies

    NARCIS (Netherlands)

    Mendoza, S. M.; Berna, J.; Perez, E. M.; Kay, E. R.; Mateo-Alonso, A.; De Nadai, C.; Zhang, S.; Baggerman, J.; Wiering, P. G.; Leigh, D. A.; Prato, M.; Brouwer, A.M.; Rudolf, P.; Nadaï, C. De

    2008-01-01

    Several rotaxanes were studied by XPS in the form of thin films or monolayers on gold substrates. Here we report a database of photoemission spectra of the C 1s, N 1s and F 1s core levels. Binding energy ranges are summarized, classifying the core levels according to the chemical groups that form pa

  11. Core level photoemission of rotaxanes: A summary on binding energies

    Energy Technology Data Exchange (ETDEWEB)

    Mendoza, S.M. [Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen (Netherlands); Berna, J.; Perez, E.M.; Kay, E.R. [School of Chemistry, University of Edinburgh, King' s Buildings, West Mains Road, Edinburgh EH9 3JJ (United Kingdom); Mateo-Alonso, A. [Dipartimento di Scienze Farmaceutiche, Universita di Trieste, Piazzale Europa 1, 34127 Trieste (Italy); De Nadai, C. [Laboratoire Interdisciplinaire de Spectroscopie Electronique, Facultes Universitaires Notre Dame de la Paix, 61 Rue de Bruxelles, B-5000 Namur (Belgium); Zhang, S. [School of Chemistry, University of Edinburgh, King' s Buildings, West Mains Road, Edinburgh EH9 3JJ (United Kingdom); Baggerman, J.; Wiering, P.G. [Van' t Hoff Institute for Molecular Sciences, University of Amsterdam, Nieuwe Achtergracht 129, NL-1018 WS Amsterdam (Netherlands); Leigh, D.A. [School of Chemistry, University of Edinburgh, King' s Buildings, West Mains Road, Edinburgh EH9 3JJ (United Kingdom); Prato, M. [Dipartimento di Scienze Farmaceutiche, Universita di Trieste, Piazzale Europa 1, 34127 Trieste (Italy); Brouwer, A.M. [Van' t Hoff Institute for Molecular Sciences, University of Amsterdam, Nieuwe Achtergracht 129, NL-1018 WS Amsterdam (Netherlands); Rudolf, P. [Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen (Netherlands)], E-mail: P.Rudolf@rug.nl

    2008-09-15

    Several rotaxanes were studied by XPS in the form of thin films or monolayers on gold substrates. Here we report a database of photoemission spectra of the C 1s, N 1s and F 1s core levels. Binding energy ranges are summarized, classifying the core levels according to the chemical groups that form part of the rotaxanes.

  12. New Connotations of Core Socialist Values at the Individual Level

    Institute of Scientific and Technical Information of China (English)

    冯正强

    2016-01-01

    The core values at the individual level including patriotism, dedication, integrity and friendship are new ethical and moral requirements put forward by the environment of new era for people;what’s more, these values propose core connotations of a new theory of human nature from the view of perfecting human nature, which indicates the recovery and manifestation of people’s feel-ings of love in the environment of a new era. This is Chinese big love, which is based on traditions and filled with new epoch con-notations. Just like warm breeze and gentle rain, Chinese big love resolves people’s indifferent hearts and moisturizes their spiritual poverty in the present era, constituting the ultimate care of the core values at the individual level.

  13. MiR-291b-3p Induces Apoptosis in Liver Cell Line NCTC1469 by Reducing the Level of RNA-binding Protein HuR

    Directory of Open Access Journals (Sweden)

    Jun Guo

    2014-03-01

    Full Text Available Background: There is increasing evidence that miRNAs are involved in cellular apoptosis. However, the specific role of miR-291b-3p in apoptosis has not been elucidated. In the present study, we investigated the effect of miR-291b-3p on NCTC1469 cell growth and apoptosis. Methods: Cell viability and apoptosis were examined in NCTC1469 cells transfected with miR-291b-3p mimics, inhibitor miRNA or negative control. Using computational miRNA target prediction databases, HuR was predicted as a target of miR-291b-3p. Luciferase assay, immunofluorescence and western blot were used to further explore the effects of miR-291b-3p on HuR expression. In addition, the effect of HuR on cell apoptosis was evaluated using a HuR-specific siRNA. Results: TNF-α-induced hepatocyte apoptosis was accompanied by enhanced expression of miR-291b-3p, suggesting that miR-291b-3p might contribute to the apoptotic process. Follow-up experiments showed that upregulation of miR-291b-3p decreased cell viability and induced NCTC1469 cell apoptosis. Additionally, similar to the activity of miR-519, which is another member of the same miRNA family, miR-291b-3p suppressed HuR translation through binding to the HuR coding region (CR. We further showed that the downregulation of HuR expression by miR-291b-3p was accompanied by reduced Bcl-2 expression. Moreover, knockdown of HuR also impaired Bcl-2 expression and increased the ratio of Bax/Bcl-2. More significantly, downregulation of miR-291b-3p failed to increase Bcl2 expression in NCTC1469 cells that were co-transfected with siRNA-HuR. Finally, inhibition of miR-291b-3p led to reduced apoptosis, while knockdown of HuR by siRNA promoted apoptosis, even in NCTC1469 cells that were co-transfected with the miR-291b-3p inhibitor. Conclusion: The current data suggested that miR-291b-3p contributed to NCTC1469 cell apoptosis by regulating the expression of HuR, which in turn increased Bcl-2 stability.

  14. Origin of metallic surface core-level shifts

    DEFF Research Database (Denmark)

    Aldén, Magnus; Skriver, Hans Lomholt; Abrikosov, I. A.

    1995-01-01

    The unique property of the open 4f energy shell in the lanthanide metals is used to show that the initial-state energy shift gives an insufficient description of surface core-level shifts. Instead a treatment, which fully includes the final-state screening, account for the experimentally observed...

  15. Up-regulation of Serum MiR-130b-3p Level is Associated with Renal Damage in Early Lupus Nephritis

    Science.gov (United States)

    Wang, Wanpeng; Mou, Shan; Wang, Ling; Zhang, Minfang; Shao, Xinghua; Fang, Wei; Lu, Renhua; Qi, Chaojun; Fan, Zhuping; Cao, Qin; Wang, Qin; Fang, Yan; Ni, Zhaohui

    2015-08-01

    Systemic lupus erythematosus (SLE) is a common but severe autoimmune systemic inflammatory disease. Lupus nephritis (LN) is a serious complication of SLE,affecting up to 70% of SLE patients. Circulating microRNAs (miRNA) are emerging as biomarkers for pathological conditions and play significant roles in intercellular communication. In present research, serum samples from healthy control, early and late stage LN patients were used to analyze the expression profile of miRNAs by microarray. Subsequent study demonstrated that miR-130b-3p in serum of patients with early stage LN were significantly up-regulated when compared with healthy controls. In addition,we have also observed that the expression of a large amount of circulating microRNAs significantly decreased in patients with late stage LN. The further analysis found that the expression of serum miR-130b-3p was positively correlated with 24-hour proteinuria and renal chronicity index in patients with early stage LN.Transfection of renal tubular cellline(HK-2)with miR-130b-3p mimics can promote epithelial-mesenchymal transition (EMT). The opposite effects were observed when transfected with miR-130b-3p inhibitors. MiR-130b-3p negatively regulated ERBB2IP expression by directly targeting the 3‧-UTR of ERBB2IP The circulating miR-130b-3p might serve as a biomarker and play an important role in renal damage in early stage LN patients.

  16. Gamma thermometer based reactor core liquid level detector

    Science.gov (United States)

    Burns, Thomas J.

    1983-01-01

    A system is provided which employs a modified gamma thermometer for determining the liquid coolant level within a nuclear reactor core. The gamma thermometer which normally is employed to monitor local core heat generation rate (reactor power), is modified by thermocouple junctions and leads to obtain an unambiguous indication of the presence or absence of coolant liquid at the gamma thermometer location. A signal processor generates a signal based on the thermometer surface heat transfer coefficient by comparing the signals from the thermocouples at the thermometer location. The generated signal is a direct indication of loss of coolant due to the change in surface heat transfer when coolant liquid drops below the thermometer location. The loss of coolant indication is independent of reactor power at the thermometer location. Further, the same thermometer may still be used for the normal power monitoring function.

  17. Core level binding energies of functionalized and defective graphene.

    Science.gov (United States)

    Susi, Toma; Kaukonen, Markus; Havu, Paula; Ljungberg, Mathias P; Ayala, Paola; Kauppinen, Esko I

    2014-01-01

    X-ray photoelectron spectroscopy (XPS) is a widely used tool for studying the chemical composition of materials and it is a standard technique in surface science and technology. XPS is particularly useful for characterizing nanostructures such as carbon nanomaterials due to their reduced dimensionality. In order to assign the measured binding energies to specific bonding environments, reference energy values need to be known. Experimental measurements of the core level signals of the elements present in novel materials such as graphene have often been compared to values measured for molecules, or calculated for finite clusters. Here we have calculated core level binding energies for variously functionalized or defected graphene by delta Kohn-Sham total energy differences in the real-space grid-based projector-augmented wave density functional theory code (GPAW). To accurately model extended systems, we applied periodic boundary conditions in large unit cells to avoid computational artifacts. In select cases, we compared the results to all-electron calculations using an ab initio molecular simulations (FHI-aims) code. We calculated the carbon and oxygen 1s core level binding energies for oxygen and hydrogen functionalities such as graphane-like hydrogenation, and epoxide, hydroxide and carboxylic functional groups. In all cases, we considered binding energy contributions arising from carbon atoms up to the third nearest neighbor from the functional group, and plotted C 1s line shapes by using experimentally realistic broadenings. Furthermore, we simulated the simplest atomic defects, namely single and double vacancies and the Stone-Thrower-Wales defect. Finally, we studied modifications of a reactive single vacancy with O and H functionalities, and compared the calculated values to data found in the literature.

  18. Surface core-level shifts for simple metals

    DEFF Research Database (Denmark)

    Aldén, Magnus; Skriver, Hans Lomholt; Johansson, Börje

    1994-01-01

    We have performed an ab initio study of the surface core-level binding energy shift (SCLS) for 11 of the simple metals by means of a Green’s-function technique within the tight-binding linear-muffin-tin-orbitals method. Initial- and final-state effects are included within the concept of complete....... We furthermore conclude that the unexpected negative sign of the SCLS in beryllium is predominantly an initial-state effect and is caused by the high electron density in this metal....

  19. Measurement of the isotope shift of the 2{{\\rm{p}}}^{4}{}^{3}{{P}}_{2} \\rightarrow 2{{\\rm{p}}}^{3}3{\\rm{p}}{}^{3}{{P}}_{2} two-photon transition of O I and a revision of the triplet energy levels of atomic oxygen

    Science.gov (United States)

    Marinov, D.; Booth, J.-P.; Drag, C.; Blondel, C.

    2017-03-01

    Two-photon induced fluorescence of atomic oxygen, which is routinely used for plasma and flame diagnostics, is implemented with two counterpropagating laser beams of sufficient monochromaticity to permit Doppler-free spectroscopy. A single-mode injection-seeded pulsed Ti:sapphire laser has been frequency-doubled twice to produce narrow-band pulsed radiation at wavelengths around 225.6 nm, suitable to excite the ground-state-to-2{{{p}}}33{{p}}{}3{P} two-photon resonance line of oxygen. Accurate measurement of the injection-seeding wavelength provides new data on the excitation energy of the 3{{p}}{}3{P} states. The experiment was done both sequentially and simultaneously on 16O and 18O, which makes it possible to test recent calculations of the isotope shift. Having an absolute measurement of the excitation wave-number from the ground level, which has been the case only twice in previous spectroscopic studies of O I, we can re-examine the energy levels of the subset of triplet states and present an updated set of optimised energy values.

  20. Relationships Between Complex Core Level Spectra and Materials Properties

    Energy Technology Data Exchange (ETDEWEB)

    Nelin, Constance J.; Bagus, Paul S.; Ilton, Eugene S.; Chambers, Scott A.; Kuhlenbeck, Helmut; Freund, Hans-Joachim

    2010-12-01

    The XPS of many oxides are quite complex and there may be several peaks of significant intensity for each subshell. These peaks arise from many-electron effects, which normally are treated with configuration interaction (CI) wavefunctions where static correlation effects are taken into account. It is common to use semiempirical methods to determine the matrix elements of the CI Hamiltonian and there are few rigorous CI calculations where parameters are not adjusted to fit experiment. In contrast, we present, in the present work, theoretical XPS spectra obtained with rigorous CI wavefunctions for CeO2 where the XPS are especially complex; several different core levels are studied. This study uses an embedded CeO8 cluster model to represent bulk CeO2 and the relativistic CI wavefunctions are determined using four-component spinors from Dirac-Fock calculations. In particular, we examine the importance of interatomic many-body effects where there is a transfer of electrons from occupied oxygen 2p orbitals into empty cation orbitals as it is common to ascribe the complex XPS to this effect. We also contrast the importance of many-body charge-transfer effects for the isoelectronic cations of Ce4+ and La3+. The long-range goal of this work is to relate the XPS features to the nature of the chemical bonding in CeO2 and we describe our progress toward this goal.

  1. GHRSST Level 3P Global Subskin Sea Surface Temperature from the Advanced Very High Resolution Radiometer (AVHRR) on the MetOp-A satellite (GDS version 1)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A global Level 3 Group for HIgh Resolution Sea Surface Temperature (GHRSST) dataset from the Advanced Very High Resolution Radiometer (AVHRR) on the MetOp-A platform...

  2. Core-level excitation and fragmentation of chlorine dioxide

    Science.gov (United States)

    Flesch, R.; Plenge, J.; Rühl, E.

    2006-03-01

    Inner-shell excitation and fragmentation of chlorine dioxide (OClO) in the Cl 2p- and O 1s-excitation regime is reported. The electronic structure of the element-selectively excited radical is studied by X-ray absorption and total cation yields. A comparison of both approaches allows us to estimate the absolute photoionization cross-section and the ionization yield near the Cl 2p- and O 1s-absorption edges. The latter quantity is characteristically enhanced in core-ionization continua. We observe below both core-absorption edges intense core-to-valence-transitions. These are assigned in comparison with related work on core-excited sulfur dioxide. These results give clear evidence that the highest molecular orbital of OClO is half-filled. High-resolution spectra recorded in the Cl 2p-regime show evidence for Rydberg transitions. The extrapolation of the term values of the low-lying Rydberg states allows us to derive the Cl 2p-ionization energy of OClO. Fragmentation of core-excited OClO is reported. Photoelectron-photoion-coincidence (PEPICO) spectra are recorded, indicating that singly and doubly charged fragments are formed. Fission of the doubly and multiply charged OClO leads to singly charged fragments. These are measured by photoion-photoion-coincidence (PIPICO) spectra, where characteristic changes in intensity of the fission channels in the Cl 2p- and O 1s-continuum are observed.

  3. Alkaline Earth Core Level Photoemission Spectroscopy of High-Temperature Superconductors

    Science.gov (United States)

    Vasquez, R.

    1993-01-01

    This paper examines photoemission measurements of the alkaline Earth core levels of high-temperature superconductors and related materials, models that seek to explain the large negative shifts observed relative to the corresponding alkaline Earth metals, and the effect of lattice site disorder on the core level spectra and the presence or absence of intrinsic surface peaks.

  4. The surface core level shift for lithium at the surface of lithium borate

    Energy Technology Data Exchange (ETDEWEB)

    Wooten, David [Air Force Institute of Technology, 2950 Hobson Way, Wright Patterson Air Force Base, OH 45433-7765 (United States); Ketsman, I.; Xiao, Jie [Department of Physics and Astronomy and the Nebraska Center for Materials and Nanoscience, University of Nebraska-Lincoln, P.O. Box 880111, Lincoln, NE 68588-0111 (United States); Losovyj, Ya.B. [Department of Physics and Astronomy and the Nebraska Center for Materials and Nanoscience, University of Nebraska-Lincoln, P.O. Box 880111, Lincoln, NE 68588-0111 (United States); J. Bennett Johnston Sr. Center for Advanced Microstructures and Devices, Louisiana State University, 6980 Jefferson Highway, Baton Rouge, LA 70806 (United States); Petrosky, J.; McClory, J. [Air Force Institute of Technology, 2950 Hobson Way, Wright Patterson Air Force Base, OH 45433-7765 (United States); Burak, Ya.V.; Adamiv, V.T. [Institute of Physical Optics, Dragomanov 23, Lviv 79005 (Ukraine); Dowben, P.A., E-mail: pdowben@unl.ed [Department of Physics and Astronomy and the Nebraska Center for Materials and Nanoscience, University of Nebraska-Lincoln, P.O. Box 880111, Lincoln, NE 68588-0111 (United States)

    2010-01-01

    The shallow Li 1s core level exhibits a surface-to-bulk core level shift for the stoichiometric Li{sub 2}B{sub 4}O{sub 7}(1 1 0) surface. Angle-resolved photoemission spectroscopy was used to indentify Li 1s bulk and surface core level components at binding energies -56.5+-0.4 and -53.7+-0.5 eV, respectively. We find photoemission evidence for surface states of Li{sub 2}B{sub 4}O{sub 7}(1 1 0) that exist in the gap of the projected bulk density of states. The existence of surface states is consistent with the large surface-to-bulk core level shift for the Li 1s core.

  5. Deep layer-resolved core-level shifts in the beryllium surface

    DEFF Research Database (Denmark)

    Aldén, Magnus; Skriver, Hans Lomholt; Johansson, Börje

    1993-01-01

    Core-level energy shifts for the beryllium surface region are calculated by means of a Green’s function technique within the tight-binding linear muffin-tin orbitals method. Both initial- and final-state effects in the core-ionization process are fully accounted for. Anomalously large energy shifts...

  6. Ab initio surface core-level shifts and surface segregation energies

    DEFF Research Database (Denmark)

    Aldén, Magnus; Skriver, Hans Lomholt; Johansson, Börje

    1993-01-01

    We have calculated the surface core-level energy shifts of the 4d and 5d transition metals by means of local-density theory and a Green’s-function technique based on the linear muffin-tin orbitals method. Final-state effects are included by treating the core-ionized atom as an impurity located...

  7. Evidence of the nature of core-level photoemission satellites using angle-resolved photoemission extended fine structure

    Energy Technology Data Exchange (ETDEWEB)

    Moler, E.J.; Kellar, S.A.; Huff, W.R.A. [Lawrence Berkeley National Lab., CA (United States)] [and others

    1997-04-01

    The authors present a unique method of experimentally determining the angular momentum and intrinsic/extrinsic origin of core-level photoemission satellites by examining the satellite diffraction pattern in the Angle Resolved Photoemission Extended Fine Structure (ARPEFS) mode. They show for the first time that satellite peaks not associated with chemically differentiated atomic species display an ARPEFS intensity oscillation. They present ARPEFS data for the carbon 1s from ({radical}3x{radical}3)R30 CO/Cu(111) and p2mg(2xl)CO/Ni(110), nitrogen 1s from c(2x2) N{sub 2}/Ni(100), cobalt 1s from p(1x1)Co/Cu(100), and nickel 3p from clean nickel (111). The satellite peaks and tails of the Doniach-Sunjic line shapes in all cases exhibit ARPEFS curves which indicate an angular momentum identical to the main peak and are of an intrinsic nature.

  8. CORE

    DEFF Research Database (Denmark)

    Krigslund, Jeppe; Hansen, Jonas; Hundebøll, Martin

    2013-01-01

    different flows. Instead of maintaining these approaches separate, we propose a protocol (CORE) that brings together these coding mechanisms. Our protocol uses random linear network coding (RLNC) for intra- session coding but allows nodes in the network to setup inter- session coding regions where flows...... intersect. Routes for unicast sessions are agnostic to other sessions and setup beforehand, CORE will then discover and exploit intersecting routes. Our approach allows the inter-session regions to leverage RLNC to compensate for losses or failures in the overhearing or transmitting process. Thus, we...... increase the benefits of XORing by exploiting the underlying RLNC structure of individual flows. This goes beyond providing additional reliability to each individual session and beyond exploiting coding opportunistically. Our numerical results show that CORE outperforms both forwarding and COPE...

  9. ZBLAN玻璃中Pr3+掺杂离子3P0和1D2能级的发光特性和寿命%Fluorescent Properties and Lifetimes of 3P0 and 1D2 Levels in Pr3+: ZBLAN Glass

    Institute of Scientific and Technical Information of China (English)

    杨海贵; 戴振文; 孙志伟; 孙桂娟

    2005-01-01

    对ZBLAN氟锆酸盐玻璃中pr3+掺杂离子3P0和1D2能级的寿命和发光特性进行了较详细的光谱学研究.首先测量了两种掺杂浓度(质量分数分别为1×10-3,5×10-3)的Pr3+:ZBLAN玻璃的吸收光谱,然后运用时间分辨激光光谱技术测量了3P0和1D2能级在激光单光子共振激发下的荧光发射谱和能级寿命.将不同荧光发射谱带的强度和文献报道的Judd-Ofelt理论计算辐射跃迁几率数值做了比较分析,证明了文献中理论计算结果的可靠性.由于浓度猝灭效应,在相同的激发条件下,掺杂浓度为1×10-3样品的荧光发射强度明显大于5×10-3样品的荧光发射强度.但是从我们的测量结果看,掺杂浓度对3P0和1D2的能级寿命值无显著影响.掺杂浓度为1×10-3时,Pr3+离子3P0和1D2能级的寿命值分别为46,322μs.

  10. Detection of subsurface core-level shifts in Si 2p core-level photoemission from Si(111)-(1x1):As

    Energy Technology Data Exchange (ETDEWEB)

    Paggel, J.J. [Philipps-Universitaet Marburg (Germany); Hasselblatt, M.; Horn, K. [Fritz-Haber Institut der Max-Planck-Gesellschraft, Berlin (Germany)] [and others

    1997-04-01

    The (7 x 7) reconstruction of the Si(111) surface arises from a lowering energy through the reduction of the number of dangling bonds. This reconstruction can be removed by the adsorption of atoms such as hydrogen which saturate the dangling bonds, or by the incorporation of atoms, such as arsenic which, because of the additional electron it possesses, can form three bonds and a nonreactive lone pair orbital from the remaining two electrons. Core and valence level photoemission and ion scattering data have shown that the As atoms replace the top silicon atoms. Previous core level spectra were interpreted in terms of a bulk and a single surface doublet. The authors present results demonstrate that the core level spectrum contains two more lines. The authors assign these to subsurface silicon layers which also experience changes in the charge distribution when a silicon atom is replaced by an arsenic atom. Subsurface core level shifts are not unexpected since the modifications of the electronic structure and/or of photohole screening are likely to decay into the bulk and not just to affect the top-most substrate atoms. The detection of subsurface components suggests that the adsorption of arsenic leads to charge flow also in the second double layer of the Si(111) surface. In view of the difference in atomic radius between As and Si, it was suggested that the (1 x 1): As surface is strained. The presence of charge rearrangement up to the second double layer implies that the atomic coordinates also exhibit deviations from their ideal Si(111) counterparts, which might be detected through a LEED I/V or photoelectron diffraction analysis.

  11. $^3P_2$ Superfluids Are Topological

    CERN Document Server

    Mizushima, Takeshi

    2016-01-01

    We clarify the topology of the $^3P_2$ superfluidity which is expected to be realized in the cores of neutron stars and cubic odd-parity superconductors. The phase diagram includes the unitary uniaxial/biaxial nematic phases and nonunitary ferromagnetic and cyclic phases. We here show that the low-energy structures of all the phases are governed by different types of topologically protected gapless fermionic excitations: Surface Majorana fermions in nematic phases, single itinerant Majorana fermion in the ferromagnetic phase, and a quartet of itinerant Majorana fermions in the cyclic phase. Using the superfluid Fermi liquid theory, we also demonstrate that dihedral-two and -four biaxial nematic phases are thermodynamically favored in the weak coupling limit under a magnetic field. The mass acquisition of surface Majorana fermions in nematic phases is subject to symmetry.

  12. Spin polarization and magnetic dichroism in core-level photoemission from ferromagnets

    Energy Technology Data Exchange (ETDEWEB)

    Menchero, Jose Gabriel [Univ. of California, Berkeley, CA (United States). Dept. of Physics

    1997-05-01

    In this thesis we present a theoretical investigation of angle- and spin-resolved core-level photoemission from ferromagnetic Fe and Ni. We also consider magneto-dichroic effects due to reversal of the photon helicity or reversal of the sample magnetization direction. In chapter 1, we provide a brief outline of the history of photoemission, and show how it has played an important role in the development of modern physics. We then review the basic elements of the theory of core-level photoemission, and discuss the validity of the some of the commonly-used approximations. In chapter 2, we present a one-electron theory to calculate spin- and angle-resolved photoemission spectra for an arbitrary photon polarization. The Hamiltonian includes both spin-orbit and exchange interactions. As test cases for the theory, we calculate the spin polarization and magnetic dichroism for the Fe 2p core level, and find that agreement with experiment is very good.

  13. Adlayer Core-Level Shifts of Random Metal Overlayers on Transition-Metal Substrates

    DEFF Research Database (Denmark)

    Ganduglia-Pirovano, M. V.; Kudrnovský, J.; Scheffler, M.

    1997-01-01

    We calculate the difference of the ionization energies of a core electron of a surface alloy, i.e., a B atom in a A(1-x)B(x) overlayer on a fee B(001) substrate, and a core electron of the clean fee B(001) surface using density-functional theory. We analyze the initial-state contributions and the...... the initial-state trends are explained in terms of the change of inter- and intra-atomic screening upon alloying. A possible role of alloying on the chemical reactivity of metal surfaces is discussed....... and the screening effects induced by the core hole, and study the influence of the alloy composition for a number of noble metal-transition metal systems. Our analysis clearly indicates the importance of final-state screening effects for the interpretation of measured core-level shifts. Calculated deviations from...

  14. Modeling Shallow Core-Level Transitions in the Reflectance Spectra of Gallium-Containing Semiconductors

    Science.gov (United States)

    Stoute, Nicholas; Aspnes, David

    2012-02-01

    The electronic structure of covalent materials is typically approached by band theory. However, shallow core level transitions may be better modeled by an atomic-scale approach. We investigate shallow d-core level reflectance spectra in terms of a local atomic-multiplet theory, a novel application of a theory typically used for higher-energy transitions on more ionic type material systems. We examine specifically structure in reflectance spectra of GaP, GaAs, GaSb, GaSe, and GaAs1-xPx due to transitions that originate from Ga3d core levels and occur in the 20 to 25 eV range. We model these spectra as a Ga^+3 closed-shell ion whose transitions are influenced by perturbations on 3d hole-4p electron final states. These are specifically spin-orbit effects on the hole and electron, and a crystal-field effect on the hole, attributed to surrounding bond charges and positive ligand anions. Empirical radial-strength parameters were obtained by least-squares fitting. General trends with respect to anion electronegativity are consistent with expectations. In addition to the spin-orbit interaction, crystal-field effects play a significant role in breaking the degeneracy of the d levels, and consequently are necessary to understand shallow 3d core level spectra.

  15. Polymers and Cross-Linking: A CORE Experiment to Help Students Think on the Submicroscopic Level

    Science.gov (United States)

    Bruce, Mitchell R. M.; Bruce, Alice E.; Avargil, Shirly; Amar, Francois G.; Wemyss, Thomas M.; Flood, Virginia J.

    2016-01-01

    The Polymers and Cross-Linking experiment is presented via a new three phase learning cycle: CORE (Chemical Observations, Representations, Experimentation), which is designed to model productive chemical inquiry and to promote a deeper understanding about the chemistry operating at the submicroscopic level. The experiment is built on two familiar…

  16. Core level excitations — A fingerprint of structural and electronic properties of epitaxial silicene

    NARCIS (Netherlands)

    Friedlein, R.; Fleurence, A.; Aoyagi, K.; Jong, de M.P.; Van Bui, H.; Wiggers, F.B.; Yoshimoto, S.; Koitaya, T.; Shimizu, S.; Noritake, H.; Mukai, K.; Yoshinobu, J.; Yamada-Takamura, Y.

    2014-01-01

    From the analysis of high-resolution Si 2p photoelectron and near-edge x-ray absorption fine structure (NEXAFS) spectra, we show that core level excitations of epitaxial silicene on ZrB2(0001) thin films are characteristically different from those of sp 3-hybridized silicon. In particular, it is rev

  17. Molecular Spectroscopy in Hollow-Core Photonic Crystal Fiber at the 10 kHz Level

    Science.gov (United States)

    Wang, Chenchen; Knabe, Kevin; Wu, Shun; Lim, Jinkang; Tillman, Karl; Washburn, Brian; Corwin, Kristan; Wheeler, Natalie; Couny, Francois; Benabid, Fetah

    2010-03-01

    High-accuracy spectroscopy in hollow-core photonic crystal fiber (HC-PCF) is desirable for many applications, including frequency references and trace gas analysis. We demonstrate the narrowest sub-Doppler linewidths attained in HC-PCF using large-core kagome structured fiber. Such fibers can yield highly accurate frequency measurements that are about two orders of magnitude higher than previously reported. A fiber laser is locked to the ^12C2H2 ν1+ν3 P(13) transition inside kagome fiber, and compared with two optical frequency combs referenced to a GPS-disciplined Rb oscillator. The absolute frequency of the measured line center agrees with those measured in power build-up cavities to within 9.3 kHz (1 σ error). Approaches to further narrow the linewidths and improve systematic errors are investigated. The present system thus combines accuracy approaching that of power build-up cavities with the potential to be compact, robust, and integrated into an all-fiber system for a portable near-infrared frequency reference. Supported by AFOSR FA9950-05-1-0304 and NSF ECS-0449295.

  18. New determination of the core-level life-time broadenings in mercury

    Energy Technology Data Exchange (ETDEWEB)

    Martensson, Nils, E-mail: nils.martensson@fysik.uu.se; Svensson, Svante

    2015-07-15

    Highlights: • We report core-level life-time widths for mercury in the gas phase. • Photoelectron spectra for the 4p, 4d, 4f and 5p levels are analyzed. • A Coster–Kronig like CI effect is observed for the 4d{sub 3/2} level. - Abstract: Previously recorded and published photoelectron spectroscopic data for mercury in the gas phase has been reanalyzed. The life-time broadenings have been determined for a large number of core levels. It is then seen that a recent detailed derivation of core-level line-widths based on X-ray emission spectroscopy give life-time widths that are generally too large. The 4d{sub 3/2}4d{sub 5/2}nd Coster–Kronig (CK) transition is also discussed. We find that the additional broadening of the 4d{sub 3/2} level for mercury metal is indeed due to a CK decay, in contrast to recent claims. In atomic mercury, however, the CK process in energetically forbidden. In spite of this we find that the 4d{sub 3/2} level is broadened also in this case. We propose that this is due to a mixing between the 4d{sub 3/2} hole state and discrete 4d{sub 5/2}nd states.

  19. High Level Analysis, Design and Validation of Distributed Mobile Systems with CoreASM

    Science.gov (United States)

    Farahbod, R.; Glässer, U.; Jackson, P. J.; Vajihollahi, M.

    System design is a creative activity calling for abstract models that facilitate reasoning about the key system attributes (desired requirements and resulting properties) so as to ensure these attributes are properly established prior to actually building a system. We explore here the practical side of using the abstract state machine (ASM) formalism in combination with the CoreASM open source tool environment for high-level design and experimental validation of complex distributed systems. Emphasizing the early phases of the design process, a guiding principle is to support freedom of experimentation by minimizing the need for encoding. CoreASM has been developed and tested building on a broad scope of applications, spanning computational criminology, maritime surveillance and situation analysis. We critically reexamine here the CoreASM project in light of three different application scenarios.

  20. Whisker-reinforced dental core buildup composites: effect of filler level on mechanical properties.

    Science.gov (United States)

    Xu, H H; Smith, D T; Schumacher, G E; Eichmiller, F C

    2000-12-15

    The strength and toughness of dental core buildup composites in large stress-bearing restorations need to be improved to reduce the incidence of fracture due to stresses from chewing and clenching. The aims of the present study were to develop novel core buildup composites reinforced with ceramic whiskers, to examine the effect of filler level, and to investigate the reinforcement mechanisms. Silica particles were fused onto the whiskers to facilitate silanization and to roughen the whisker surface for improved retention in the matrix. Filler level was varied from 0 to 70%. Flexural strength, compressive strength, and fracture toughness of the composites were measured. A nano-indentation system was used to measure elastic modulus and hardness. Scanning electron microscopy (SEM) was used to examine the fracture surfaces of specimens. Whisker filler level had significant effects on composite properties. The flexural strength in MPa (mean +/- SD; n = 6) increased from (95+/-15) for the unfilled resin to (193+/- 8) for the composite with 50% filler level, then slightly decreased to (176+/-12) at 70% filler level. The compressive strength increased from (149+/-33) for the unfilled resin to (282+/-48) at 10% filler level, and remained equivalent from 10 to 70% filler level. Both the modulus and hardness increased monotonically with filler level. In conclusion, silica particle-fused ceramic single-crystalline whiskers significantly reinforced dental core buildup composites. The reinforcement mechanisms appeared to be crack deflection and bridging by the whiskers. Whisker filler level had significant effects on the flexural strength, compressive strength, elastic modulus, and hardness of composites.

  1. Core-level magnetic circular dichroism in 3d and 4f magnetic systems (invited) (abstract)

    Science.gov (United States)

    Koide, T.

    1994-05-01

    With the recent availability of circulary polarized synchrotron radiation over a wide photon energy range from VUV to hard X rays, the magnetic circular dichroism (MCD) in core-level photoabsorption has rapidly attracted growing interest, both experimentally and theoretically. This novel technique can provide element-specific and site-selective information about the magnetic and the electronic states in various magnetic substances because the core-level MCD process involves optical transitions in which the one-electron initial states are well localized and have well-defined angular momenta. In order to get insight into the local magnetic states in 3d and 4f magnetic systems, we have studied MCD of ferrites, Fe1-xPtx alloys, and mixed-valence CeRh3B2 at the core-absorption edges in the VUV˜soft x-ray region. The experiments were performed by utilizing directly characterized, circularly polarized undulator radiation and off-plane synchrotron radiation1 in conjunction with an ultrahigh vacuum compatible superconducting magnet of special design.2 Clear MCD signals were observed for CeRh3B2 in the prethreshold region of the Ce 4d→4f (N4,5) edges. A comparison of the experimental MCD spectrum with theoretical ones3 for uniaxial crystal fields of Δc=0 and 0.2 eV shows that the experimental spectrum qualitatively agrees with the theoretical one for Δc=0 eV. Theory predicts that the MCD pattern for ΔcCeRh3B2. We will also present the MCD data in the M2,3 core-absorption region for ferrites (Fe3O4 and CoFe2O4) and Fe1-xPtx alloys, discussing the results.

  2. Identifying heavy metal levels in historical flood water deposits using sediment cores.

    Science.gov (United States)

    Lintern, Anna; Leahy, Paul J; Heijnis, Henk; Zawadzki, Atun; Gadd, Patricia; Jacobsen, Geraldine; Deletic, Ana; Mccarthy, David T

    2016-11-15

    When designing mitigation and restoration strategies for aquatic systems affected by heavy metal contamination, we must first understand the sources of these pollutants. In this study, we introduce a methodology that identifies the heavy metal levels in floodplain lake sediments deposited by one source; fluvial floods. This is done by comparing sediment core heavy metal profiles (i.e., historical pollution trends) to physical and chemical properties of sediments in these cores (i.e., historical flooding trends). This methodology is applied to Willsmere and Bolin Billabongs, two urban floodplain lakes (billabongs) of the Yarra River (South-East Australia). Both billabongs are periodically inundated by flooding of the Yarra River and one billabong (Willsmere Billabong) is connected to an urban stormwater drainage network. 1-2-m long sediment cores (containing sediment deposits up to 500 years old) were taken from the billabongs and analysed for heavy metal concentrations (arsenic, chromium, copper, lead, nickel, zinc). In cores from both billabongs, arsenic concentrations are high in the flood-borne sediments. In Bolin Billabong, absolute metal levels are similar in flood and non-flood deposits. In Willsmere Billabong, absolute copper, lead and zinc levels were generally lower in fluvial flood-borne sediments in the core compared to non-fluvial sediments. This suggests that heavy metal concentrations in Bolin Billabong sediments are relatively similar regardless of whether or not fluvial flooding is occurring. However for Willsmere Billabong, heavy metal concentrations are high when overland runoff, direct urban stormwater discharges or atmospheric deposition is occurring. As such, reducing the heavy metal concentrations in these transport pathways will be of great importance when trying to reduce heavy metal concentrations in Willsmere Billabong sediments. This study presents a proof-of-concept that can be applied to other polluted aquatic systems, to understand the

  3. Ultra-sensitive Flow Injection Analysis (FIA) determination of calcium in ice cores at ppt level.

    Science.gov (United States)

    Traversi, R; Becagli, S; Castellano, E; Maggi, V; Morganti, A; Severi, M; Udisti, R

    2007-07-02

    A Flow Injection Analysis (FIA) spectrofluorimetric method for calcium determination in ice cores was optimised in order to achieve better analytical performances which would make it suitable for reliable calcium measurements at ppt level. The method here optimised is based on the formation of a fluorescent compound between Ca and Quin-2 in buffered environment. A careful evaluation of operative parameters (reagent concentration, buffer composition and concentration, pH), influence of interfering species possibly present in real samples and potential favourable effect of surfactant addition was carried out. The obtained detection limit is around 15 ppt, which is one order of magnitude lower than the most sensitive Flow Analysis method for Ca determination currently available in literature and reproducibility is better than 4% for Ca concentrations of 0.2 ppb. The method was validated through measurements performed in parallel with Ion Chromatography on 200 samples from an alpine ice core (Lys Glacier) revealing an excellent fit between the two chemical series. Calcium stratigraphy in Lys ice core was discussed in terms of seasonal pattern and occurrence of Saharan dust events.

  4. Production Level CFD Code Acceleration for Hybrid Many-Core Architectures

    Science.gov (United States)

    Duffy, Austen C.; Hammond, Dana P.; Nielsen, Eric J.

    2012-01-01

    In this work, a novel graphics processing unit (GPU) distributed sharing model for hybrid many-core architectures is introduced and employed in the acceleration of a production-level computational fluid dynamics (CFD) code. The latest generation graphics hardware allows multiple processor cores to simultaneously share a single GPU through concurrent kernel execution. This feature has allowed the NASA FUN3D code to be accelerated in parallel with up to four processor cores sharing a single GPU. For codes to scale and fully use resources on these and the next generation machines, codes will need to employ some type of GPU sharing model, as presented in this work. Findings include the effects of GPU sharing on overall performance. A discussion of the inherent challenges that parallel unstructured CFD codes face in accelerator-based computing environments is included, with considerations for future generation architectures. This work was completed by the author in August 2010, and reflects the analysis and results of the time.

  5. CAIA level I an introduction to core topics in alternative investments

    CERN Document Server

    Anson, Mark J P; CAIA Association; Black, Keith H; Kazemi, Hossein

    2012-01-01

    The official study text for the Level I Chartered Alternative Investment Analyst (CAIA) exam The Chartered Alternative Investment Analyst (CAIA) designation is the financial industry's first and only globally recognized program that prepares professionals to deal with the ever-growing field of alternative investments. The second edition of CAIA Level I: An Introduction to Core Topics in Alternative Investments contains comprehensive insights on the alternative investment issues a potential Level I candidate would need to know about as they prepare for the exam. The information found here will help you build a solid foundation in alternative investment markets--with coverage of everything from the characteristics of various strategies within each alternative asset class to portfolio management concepts central to alternative investments. * Uses investment analytics to examine each alternative asset class * Examines quantitative techniques used by investment professionals * Addresses the unique attributes a...

  6. Meeting the International Health Regulations (2005) surveillance core capacity requirements at the subnational level in Europe

    DEFF Research Database (Denmark)

    Ziemann, Alexandra; Rosenkötter, Nicole; Riesgo, Luis Garcia-Castrillo;

    2015-01-01

    public health emergencies of international concern: (i) can syndromic surveillance support countries, especially the subnational level, to meet the International Health Regulations (2005) core surveillance capacity requirements, (ii) are European syndromic surveillance systems comparable to enable cross......-border surveillance, and (iii) at which administrative level should syndromic surveillance best be applied? DISCUSSION: Despite the ongoing criticism on the usefulness of syndromic surveillance which is related to its clinically nonspecific output, we demonstrate that it was a suitable supplement for timely...... assessment of the impact of three different public health emergencies affecting Europe. Subnational syndromic surveillance analysis in some cases proved to be of advantage for detecting an event earlier compared to national level analysis. However, in many cases, syndromic surveillance did not detect local...

  7. First-principles calculation of core-level binding energy shift in surface chemical processes

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Combined with third generation synchrotron radiation light sources, X-ray photoelectron spectroscopy (XPS) with higher energy resolution, brilliance, enhanced surface sensitivity and photoemission cross section in real time found extensive applications in solid-gas interface chemistry. This paper reports the calculation of the core-level binding energy shifts (CLS) using the first-principles density functional theory. The interplay between the CLS calculations and XPS measurements to uncover the structures, adsorption sites and chemical reactions in complex surface chemical processes are highlight. Its application on clean low index (111) and vicinal transition metal surfaces, molecular adsorption in terms of sites and configuration, and reaction kinetics are domonstrated.

  8. Effects of vibrational motion on core-level spectra of prototype organic molecules

    Energy Technology Data Exchange (ETDEWEB)

    Uejio, Janel S.; Schwartz, Craig P.; Saykally, Richard J.; Prendergast, David

    2008-08-21

    A computational approach is presented for prediction and interpretation of core-level spectra of complex molecules. Applications are presented for several isolated organic molecules, sampling a range of chemical bonding and structural motifs. Comparison with gas phase measurements indicate that spectral lineshapes are accurately reproduced both above and below the ionization potential, without resort to ad hoc broadening. Agreement with experiment is significantly improved upon inclusion of vibrations via molecular dynamics sampling. We isolate and characterize spectral features due to particular electronic transitions enabled by vibrations, noting that even zero-point motion is sufficient in some cases.

  9. Core level excitations—A fingerprint of structural and electronic properties of epitaxial silicene

    Energy Technology Data Exchange (ETDEWEB)

    Friedlein, R., E-mail: friedl@jaist.ac.jp; Fleurence, A.; Aoyagi, K.; Yamada-Takamura, Y. [School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), 1-1, Asahidai, Nomi, Ishikawa 923-1292 (Japan); Jong, M. P. de; Van Bui, H.; Wiggers, F. B. [MESA Institute for Nanotechnology, University of Twente, 7500 AE Enschede (Netherlands); Yoshimoto, S.; Koitaya, T.; Shimizu, S.; Noritake, H.; Mukai, K.; Yoshinobu, J. [The Institute for Solid State Physics, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8581 (Japan)

    2014-05-14

    From the analysis of high-resolution Si 2p photoelectron and near-edge x-ray absorption fine structure (NEXAFS) spectra, we show that core level excitations of epitaxial silicene on ZrB{sub 2}(0001) thin films are characteristically different from those of sp{sup 3}-hybridized silicon. In particular, it is revealed that the lower Si 2p binding energies and the low onset in the NEXAFS spectra as well as the occurrence of satellite features in the core level spectra are attributed to the screening by low-energy valence electrons and interband transitions between π bands, respectively. The analysis of observed Si 2p intensities related to chemically distinct Si atoms indicates the presence of at least one previously unidentified component. The presence of this component suggests that the observation of stress-related stripe domains in scanning tunnelling microscopy images is intrinsically linked to the relaxation of Si atoms away from energetically unfavourable positions.

  10. The Systems Biology Markup Language (SBML) Level 3 Package: Layout, Version 1 Core.

    Science.gov (United States)

    Gauges, Ralph; Rost, Ursula; Sahle, Sven; Wengler, Katja; Bergmann, Frank Thomas

    2015-09-04

    Many software tools provide facilities for depicting reaction network diagrams in a visual form. Two aspects of such a visual diagram can be distinguished: the layout (i.e.: the positioning and connections) of the elements in the diagram, and the graphical form of the elements (for example, the glyphs used for symbols, the properties of the lines connecting them, and so on). For software tools that also read and write models in SBML (Systems Biology Markup Language) format, a common need is to store the network diagram together with the SBML representation of the model. This in turn raises the question of how to encode the layout and the rendering of these diagrams. The SBML Level 3 Version 1 Core specification does not provide a mechanism for explicitly encoding diagrams, but it does provide a mechanism for SBML packages to extend the Core specification and add additional syntactical constructs. The Layout package for SBML Level 3 adds the necessary features to SBML so that diagram layouts can be encoded in SBML files, and a companion package called SBML Rendering specifies how the graphical rendering of elements can be encoded. The SBML Layout package is based on the principle that reaction network diagrams should be described as representations of entities such as species and reactions (with direct links to the underlying SBML elements), and not as arbitrary drawings or graphs; for this reason, existing languages for the description of vector drawings (such as SVG) or general graphs (such as GraphML) cannot be used.

  11. Quanty for core level spectroscopy - excitons, resonances and band excitations in time and frequency domain

    Science.gov (United States)

    Haverkort, Maurits W.

    2016-05-01

    Depending on the material and edge under consideration, core level spectra manifest themselves as local excitons with multiplets, edge singularities, resonances, or the local projected density of states. Both extremes, i.e., local excitons and non-interacting delocalized excitations are theoretically well under control. Describing the intermediate regime, where local many body interactions and band-formation are equally important is a challenge. Here we discuss how Quanty, a versatile quantum many body script language, can be used to calculate a variety of different core level spectroscopy types on solids and molecules, both in the frequency as well as the time domain. The flexible nature of Quanty allows one to choose different approximations for different edges and materials. For example, using a newly developed method merging ideas from density renormalization group and quantum chemistry [1-3], Quanty can calculate excitons, resonances and band-excitations in x-ray absorption, photoemission, x-ray emission, fluorescence yield, non-resonant inelastic x-ray scattering, resonant inelastic x-ray scattering and many more spectroscopy types. Quanty can be obtained from: http://www.quanty.org.

  12. Photoemission and core-level magnetic circular dichroism studies of diluted magnetic semiconductors

    Energy Technology Data Exchange (ETDEWEB)

    Fujimori, A. [Department of Complexity Science and Engineering, Universtiy of Tokyo, 1-5-1 Kashiwanoha, Kashiwa, Chiba 277-8561 (Japan) and Synchrotron Radiation Research Center, Japan Atomic Energy Research Institute, SPring-8, Mikazuki, Hyogo 679-5148 (Japan)]. E-mail: fujimori@phys.s.u-tokyo.ac.jp; Okabayashi, J. [Department of Applied Chemistry, University of Tokyo, 7-3-1 Hongo, Bunkyou-ku, Tokyo 113-8656 (Japan); Takeda, Y. [Synchrotron Radiation Research Center, Japan Atomic Energy Research Institute, SPring-8, Mikazuki, Hyogo 679-5148 (Japan); Mizokawa, T. [Department of Complexity Science and Engineering, Universtiy of Tokyo, 1-5-1 Kashiwanoha, Kashiwa, Chiba 277-8561 (Japan); Okamoto, J. [Synchrotron Radiation Research Center, Japan Atomic Energy Research Institute, SPring-8, Mikazuki, Hyogo 679-5148 (Japan); Mamiya, K. [Photon Factory, IMSS, High Energy Accelerator Research Organization, Tsukuba, Ibaraki 305 (Japan); Saitoh, Y. [Synchrotron Radiation Research Center, Japan Atomic Energy Research Institute, SPring-8, Mikazuki, Hyogo 679-5148 (Japan); Muramatsu, Y. [Synchrotron Radiation Research Center, Japan Atomic Energy Research Institute, SPring-8, Mikazuki, Hyogo 679-5148 (Japan); Oshima, M. [Department of Applied Chemistry, University of Tokyo, 7-3-1 Hongo, Bunkyou-ku, Tokyo 113-8656 (Japan); Ohya, S. [Department of Electronic Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan); Tanaka, M. [Department of Electronic Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656 (Japan)

    2005-06-15

    An overview is given on the photoemission studies of the electronic structure of diluted magnetic semiconductors (DMS's), in particular of the prototypical ferromagnetic DMS Ga{sub 1-x}Mn{sub x}As. Configuration-interaction cluster-model analyses of the photoemission data allow us to estimate the p-d exchange coupling constant and hence to predict how to increase the Curie temperature in new materials. Spectra near the Fermi level combined with the transport and optical properties suggest a highly incoherent metallic state for the ferromagnetic metallic phase. It is shown that new insight into the chemically and magnetically inhomogeneous states of DMS's can be gained by the temperature and magnetic field dependence of core-level magnetic circular dichroism signals.

  13. Wettability evaluation of a carbonate reservoir rock from core to pore level

    Energy Technology Data Exchange (ETDEWEB)

    Al-Yousef, H.Y.; Lichaa, P.M.; Al-Kaabi, A.U.; Alpustun, H.

    1995-10-01

    Wettability evaluation was performed during stags of as received, cleaned, and restored states on core samples form a Saudi Arabian carbonate reservoir. The wettability behavior from the chemistry of brine-oil-carbonate rock interaction was found to be neutral to slightly oil-wet. The pore-size distribution obtained from mercury injection data indicated that about 15--20 percent of the pore volume is not accessible to asphaltene particles in the crude oil. Therefore a mixed-wettability state can exist. These results were confirmed by the evaluation of the wettability of the cores using USBM and Amott techniques. The wettability at the pore level was studied using Cryo-Scanning Electron Microscopy. Rock samples were examined at irreducible water and residual oil saturations during cleaned and restored states. At irreducible water saturation, both oil and brine were present in the intergranular macroporosity and intragranular macropores and micropores. At residual oil saturation,oil was found in the form of isolated globules in the cleaned case. After aging, the oil is more loosely distributed in the porosity and generally contacts the pore walls. This indicates an evolution toward oil wetness with aging. These results are in agreement with the changes of wettability indices obtained using USBM technique.

  14. Core-level electronic properties of nanostructured NiO coatings

    Energy Technology Data Exchange (ETDEWEB)

    Palacin, S. [Departamento de Fisica Aplicada, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid (Spain); Gutierrez, A. [Departamento de Fisica Aplicada, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid (Spain)], E-mail: a.gutierrez@uam.es; Preda, I.; Hernandez-Velez, M. [Departamento de Fisica Aplicada, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid (Spain); Sanz, R. [Instituto de Ciencia de Materiales, CSIC, Cantoblanco, 28049 Madrid (Spain); Jimenez, J.A. [Centro Nacional de Investigaciones Metalurgicas, CSIC, Av. Gregorio del Amo 8, 28040 Madrid (Spain); Soriano, L. [Departamento de Fisica Aplicada, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid (Spain)

    2007-10-31

    Nanostructured NiO films with different thicknesses were grown on nanoporous alumina membrane substrates by reactive evaporation of Ni in an oxygen atmosphere. The reactive deposition process was assisted by a low energy oxygen ion-beam in order to increase the NiO input into the pores. Surface morphology and structure of the films were analyzed by SEM and XPS. SEM observations reveal a well adhered film of NiO on the substrate. This film appears to be uniform and presents a rather irregular nanostructured morphology, built of NiO clusters with sizes ranging between 5 and 30 nm. The core-level electronic properties of this nanostructured NiO film result to be similar to those of an ultrathin film about one monolayer thick. This behaviour can be explained by the large surface to volume ratio of both systems.

  15. Exploring the core level shift origin of sulfur and thiolates on Pd(111) surfaces.

    Science.gov (United States)

    Salvarezza, Roberto Carlos; Carro, Pilar

    2015-10-01

    Thiol molecules on planar metal surfaces are widely used for building sensing and electronic devices and also as capping agents to protect and to control the size and shape of nanoparticles. In the case of Pd the thiol molecules exhibit a complex behavior because C-S bond scission is possible, resulting in a significant amount of co-adsorbed S. Therefore identification of these species on Pd is a key point for many applications, a task that is usually achieved by XPS. Here we show, from DFT calculations, that the core level shift (CLS) of the S 2p binding energy (BE) of thiol and sulfur on different thiol-Pd(111) surface models strongly depends on the adsorbed or subsurface state of sulfur atoms. Our results reflect the complexity of S 2p BE behavior and contribute to understanding and reanalyzing the experimental data of thiolated Pd surfaces.

  16. Characterization of tuyere-level core-drill coke samples from blast furnace operation

    Energy Technology Data Exchange (ETDEWEB)

    S. Dong; N. Paterson; S.G. Kazarian; D.R. Dugwell; R. Kandiyoti [Imperial College London, London (United Kingdom). Department of Chemical Engineering

    2007-12-15

    A suite of tuyere-level coke samples have been withdrawn from a working blast furnace during coal injection, using the core-drilling technique. The samples have been characterized by size exclusion chromatography (SEC), Fourier transform Raman spectroscopy (FT-RS), and X-ray powder diffraction (XRD) spectroscopy. The 1-methyl-2-pyrrolidinone (NMP) extracts of the cokes sampled from the 'bosh', the rear of the 'bird's nest', and the 'dead man' zones were found by SEC to contain heavy soot-like materials (ca. 10{sup 7}-10{sup 8} apparent mass units). In contrast, NMP extracts of cokes taken from the raceway and the front of the 'bird's nest' only contained a small amount of material of relatively lower apparent molecular mass (up to ca. 10{sup 5} u). Since the feed coke contained no materials extractable by the present method, the soot-like materials are thought to have formed during the reactions of volatile matter released from the injectant coal, probably via dehydrogenation and repolymerization of the tars. The Raman spectra of the NMP-extracted core-drilled coke samples showed variations reflecting their temperature histories. Area ratios of D-band to G-band decreased as the exposure temperature increased, while intensity ratios of D to G band and those of 2D to G bands increased with temperature. The graphitic (G), defect (D), and random (R) fractions of the carbon structure of the cokes were also derived from the Raman spectra. The R fractions decreased with increasing temperature, whereas G fractions increased, while the D fractions showed a more complex variation with temperature. These data appear to give clues regarding the graphitization mechanism of tuyere-level cokes in the blast furnace. 41 refs., 9 figs., 6 tabs.

  17. Core-Level Modeling and Frequency Prediction for DSP Applications on FPGAs

    Directory of Open Access Journals (Sweden)

    Gongyu Wang

    2015-01-01

    Full Text Available Field-programmable gate arrays (FPGAs provide a promising technology that can improve performance of many high-performance computing and embedded applications. However, unlike software design tools, the relatively immature state of FPGA tools significantly limits productivity and consequently prevents widespread adoption of the technology. For example, the lengthy design-translate-execute (DTE process often must be iterated to meet the application requirements. Previous works have enabled model-based, design-space exploration to reduce DTE iterations but are limited by a lack of accurate model-based prediction of key design parameters, the most important of which is clock frequency. In this paper, we present a core-level modeling and design (CMD methodology that enables modeling of FPGA applications at an abstract level and yet produces accurate predictions of parameters such as clock frequency, resource utilization (i.e., area, and latency. We evaluate CMD’s prediction methods using several high-performance DSP applications on various families of FPGAs and show an average clock-frequency prediction error of 3.6%, with a worst-case error of 20.4%, compared to the best of existing high-level prediction methods, 13.9% average error with 48.2% worst-case error. We also demonstrate how such prediction enables accurate design-space exploration without coding in a hardware-description language (HDL, significantly reducing the total design time.

  18. Thermoelastic properties of Zn3P2

    DEFF Research Database (Denmark)

    Gerward, Leif; Olsen, J. Staun; Waśkowska, A.

    2011-01-01

    The bulk modulus and thermal expansion of Zn3P2 has been investigated at pressures up to 21GPa and temperatures down to 100K. The experimental zero-pressure bulk modulus is 80.7 ± 1.8GPa, in accordance with the bulk modulus scaling and lattice properties of the related compound Cd3P2. A tetragonal...... to orthorhombic phase transformation occurs above 11GPa with a relative volume change of-7.1%. Values for the thermal expansion coefficient are reported at 293, 200 and 100K....

  19. Optimisation of glaciological parameters for ice core chronology by implementing counted layers between identified depth levels

    Science.gov (United States)

    Bazin, L.; Lemieux-Dudon, B.; Landais, A.; Guillevic, M.; Kindler, P.; Parrenin, F.; Martinerie, P.

    2014-08-01

    A~recent coherent chronology has been built for 4 Antarctic ice cores and the NorthGRIP (NGRIP) Greenland ice core (Antarctic Ice Core Chronology 2012, AICC2012) using a bayesian approach for ice core dating (Datice). When building the AICC2012 chronology, and in order to prevent any confusion with official ice cores chronology, it has been imposed that the AICC2012 chronology for NGRIP should respect exactly the GICC05 chronology based on layer counting. However, such a strong tuning did not satisfy the hypothesis of independence of background parameters and observations for the NGRIP core as required by Datice. We present here the implementation in Datice of a new type of markers that is better suited to constraints deduced from layer counting: the markers of age-difference. Using this type of markers for NGRIP in a 5 cores dating exercise with Datice, we have performed several sensitivity tests and show that the new ice core chronologies obtained with these new markers do not differ by more than 400 years from AICC2012 for Antarctic ice cores and by more than 130 years from GICC05 for NGRIP over the last 60 000 years. With this new parameterization, the accumulation rate and lock-in depth associated with NGRIP are more coherent with independent estimates than those obtained in AICC2012. While these new chronologies should not be used yet as new ice core chronologies, the improved methodology presented here should be considered in the next coherent ice core dating exercise.

  20. Uncertainties of the neutronic calculations at core level determined by the KARATE code system and the KIKO3D code

    Energy Technology Data Exchange (ETDEWEB)

    Panka, Istvan; Kereszturi, Andras [Hungarian Academy of Sciences, Budapest (Hungary). Reactor Analysis Dept.

    2013-09-15

    In this paper the uncertainties of the neutronic calculations at core level - originating from the uncertainties of the basic nuclear data - are presented. The investigations have been made for a VVER-1000 core (Kozloduy-6) defined in the frame of the OECD NEA UAM benchmark. In the first part of the paper, the uncertainties of the effective multiplication factor, the assembly-wise radial power distribution, the axial power distribution and the rod worth are shown. After that the preliminary evaluation of the uncertainties of the neutron kinetic calculations are presented for a rod movement transient at HZP (Hot Zero Power) state, where the uncertainties of the time dependent core and assembly powers and the dynamic reactivity were evaluated. In both cases, we will see that the most important quantities - at core level and at HZP state - have a considerable uncertainty which is originating from the uncertainties of the basic cross section library in these investigations. (orig.)

  1. Usage Level of Non-Farsi Periodicals within Arts Library and Determination of Core Journals

    OpenAIRE

    Saadat Ahmadzadeh

    2007-01-01

    The present study was conducted to determine the core non-farsi periodicals within the library of the Faculty of Fine Arts in University of Tehran. 125 non-farsi journal subscriptions for 2004 were selected. 150 graduate students and 50 faculty members were surveyed. Bibliometric method along with statistical analysis were employed to determine the core journals. Journals within any given field were ranked by both students and the faculty. Core journals were matched against these ranked journ...

  2. 50 years anniversary of the discovery of the core level chemical shifts. The early years of photoelectron spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Mårtensson, Nils [Department of Physics and Astronomy, Uppsala University, Box 516, 751 20 Uppsala (Sweden); Sokolowski, Evelyn [Tvär-Ramsdal 1, 611 99 Tystberga (Sweden); Svensson, Svante, E-mail: Svante.Svensson@fysik.uu.se [Department of Physics and Astronomy, Uppsala University, Box 516, 751 20 Uppsala (Sweden)

    2014-03-01

    Highlights: • 50 years since the discovery of t the core level chemical shift. • The pioneering years of ESCA. • A critical review of the first core electron chemical shift results. - Abstract: The pioneering years of photoelectron spectroscopy in Uppsala are discussed, especially the work leading to the discovery of the core level chemical shifts. At a very early stage of the project, the pioneering group observed what they described as evidence for chemical shifts in the core level binding energies. However, it can now be seen that the initial observations to a large extent was due to charging of the samples. It is interesting to note that the decisive experiment was realized, not as a result of a systematic study, but was obtained with a large element of serendipity. Only when a chemical binding energy shift was observed between two S2p electron lines in the same molecule, the results were accepted internationally, and the fascinating expansion of modern core level photoelectron spectroscopy could start.

  3. Can circular dichroism in core-level photoemission provide a spectral fingerprint of adsorbed chiral molecules?

    Energy Technology Data Exchange (ETDEWEB)

    Allegretti, F [Physics Department, University of Warwick, Coventry CV4 7AL (United Kingdom); Polcik, M [Fritz-Haber-Institut der Max-Planck-Gesellschaft, Faradayweg 4-6, D 14195 Berlin (Germany); Sayago, D I [Fritz-Haber-Institut der Max-Planck-Gesellschaft, Faradayweg 4-6, D 14195 Berlin (Germany); Demirors, F [Fritz-Haber-Institut der Max-Planck-Gesellschaft, Faradayweg 4-6, D 14195 Berlin (Germany); O' Brien, S [Physics Department, University of Warwick, Coventry CV4 7AL (United Kingdom); Nisbet, G [Centre for Applied Catalysis, Department of Chemical and Biological Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH (United Kingdom); Lamont, C L A [Centre for Applied Catalysis, Department of Chemical and Biological Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH (United Kingdom); Woodruff, D P [Physics Department, University of Warwick, Coventry CV4 7AL (United Kingdom)

    2005-04-01

    The results of experimental measurements and theoretical simulations of circular dichroism in the angular distribution (CDAD) of photoemission from atomic core levels of each of the enantiomers of a chiral molecule, alanine, adsorbed on Cu(1 1 0) are presented. Measurements in, and out of, substrate mirror planes allow one to distinguish the CDAD due to the chirality of the sample from that due to a chiral experimental geometry. For these studies of oriented chiral molecules, the CDAD is seen not only in photoemission from the molecular chiral centre, but also from other atoms which have chiral geometries as a result of the adsorption. The magnitude of the CDAD due to the sample chirality differs for different adsorption phases of alanine, and for different emission angles and energies, but is generally small compared with CDAD out of the substrate mirror planes which is largely unrelated to the molecular chirality. While similar measurements of other molecules may reveal larger CDAD due to molecular chirality, the fact that the results for one chiral molecule show weak effects means that such CDAD is unlikely to provide a simple and routine general spectral fingerprint of adsorbed molecular chirality.

  4. Core level photoelectron spectroscopy on the lanthanide-induced hydrolysis of DNA

    Science.gov (United States)

    Shigekawa, Hidemi; Ikawa, Hiroyuki; Yoshizaki, Ryozo; Iijima, Yoshitoki; Sumaoka, Jun; Komiyama, Makoto

    1996-03-01

    The electronic structures of the complexes of diphenyl phosphate (DPP), a model compound of DNA, with lanthanide ions have been investigated to shed light on the mechanism of the cerium (IV)-induced nonenzymatic hydrolysis of DNA. Binding energies of the P 2p core level of DPP were 134.2 eV for the complexes with La(III), Eu(III), and Lu(III), and was 134.4 eV for the Ce(IV) complex, when the metal/DPP molar ratio was 1:1. When the molar ratio was increased, only Ce(IV), the most active metal ion for DNA hydrolysis, showed a chemical shift of ˜0.5 eV toward the higher binding energy region. The chemical shift of ˜0.5 eV toward the higher binding energy region. The chemical shift was due to the systematic increase in the intensity of the higher binding energy component. The observed change in the electronic structure of the DPP-Ce(IV) complex may be related to the superb ability of Ce(IV) for the hydrolysis of DNA.

  5. The Systems Biology Markup Language (SBML): Language Specification for Level 3 Version 1 Core.

    Science.gov (United States)

    Hucka, Michael; Bergmann, Frank T; Hoops, Stefan; Keating, Sarah M; Sahle, Sven; Schaff, James C; Smith, Lucian P; Wilkinson, Darren J

    2015-09-04

    Computational models can help researchers to interpret data, understand biological function, and make quantitative predictions. The Systems Biology Markup Language (SBML) is a file format for representing computational models in a declarative form that can be exchanged between different software systems. SBML is oriented towards describing biological processes of the sort common in research on a number of topics, including metabolic pathways, cell signaling pathways, and many others. By supporting SBML as an input/output format, different tools can all operate on an identical representation of a model, removing opportunities for translation errors and assuring a common starting point for analyses and simulations. This document provides the specification for Version 1 of SBML Level 3 Core. The specification defines the data structures prescribed by SBML as well as their encoding in XML, the eXtensible Markup Language. This specification also defines validation rules that determine the validity of an SBML document, and provides many examples of models in SBML form. Other materials and software are available from the SBML project web site, http://sbml.org/.

  6. Raising FLAGS: Renewing Core French at the Pre-service Teacher Level

    Directory of Open Access Journals (Sweden)

    Wendy Carr

    2010-02-01

    Full Text Available A new program for core French teacher candidates called FLAGS (French Language and Global Studies was established at the University of British Columbia (UBC in 2007. The program is intended for those who are keen to teach core French and possess rudimentary proficiency in the language but may not necessarily have the same proficiency or prior coursework as candidates applying to existing French specialist cohorts. The FLAGS program begins with a five-week summer immersion experience through Explore! and then proceeds with UBC's regular Bachelor of Education program but with an added French conversation course, a core French methodology course and a core French practicum concentration (augmented, in some cases, with a three-week practicum in a francophone locale. FLAGS is intended to address the less than satisfactory state of elementary core French teaching and learning in British Columbia.

  7. Engineering task plan for upgrades to the leveling jacks on core sample trucks number 3 and 4

    Energy Technology Data Exchange (ETDEWEB)

    KOSTELNIK, A.J.

    1999-02-24

    Characterizing the waste in underground storage tanks at the Hanford Site is accomplished by obtaining a representative core sample for analysis. Core sampling is one of the numerous techniques that have been developed for use given the environmental and field conditions at the Hanford Site. Core sampling is currently accomplished using either Push Mode Core Sample Truck No.1 or; Rotary Mode Core Sample Trucks No.2, 3 or 4. Past analysis (WHC 1994) has indicated that the Core Sample Truck (CST) leveling jacks are structurally inadequate when lateral loads are applied. WHC 1994 identifies many areas where failure could occur. All these failures are based on exceeding the allowable stresses listed in the American Institute of Steel Construction (AISC) code. The mode of failure is for the outrigger attachments to the truck frame to fail resulting in dropping of the CST and possible overturning (Ref. Ziada and Hundal, 1996). Out of level deployment of the truck can exceed the code allowable stresses in the structure. Calculations have been performed to establish limits for maintaining the truck level when lifting. The calculations and the associated limits are included in appendix A. The need for future operations of the CSTS is limited. Sampling is expected to be complete in FY-2001. Since there is limited time at risk for continued use of the CSTS with the leveling controls without correcting the structural problems, there are several design changes that could give incremental improvements to the operational safety of the CSTS with limited impact on available operating time. The improvements focus on making the truck easier to control during lifting and leveling. Not all of the tasks identified in this ETP need to be performed. Each task alone can improve the safety. This engineering task plan is the management plan document for implementing the necessary additional structural analysis. Any additional changes to meet requirements of standing orders shall require a

  8. Comparative study of the core level photoemission of the ZrB{sub 2} and ZrB{sub 12}

    Energy Technology Data Exchange (ETDEWEB)

    Huerta, L. [Instituto de Investigaciones en Materiales, Universidad Nacional Autonoma de Mexico, 04510 Mexico D.F. (Mexico); Duran, A. [Centro de Nanociencias y Nanotecnologia, Universidad Nacional Autonoma de Mexico, Apartado Postal 2681, Ensenada, Baja California 22800 (Mexico); Falconi, R. [Division Academica de Ciencias Basicas, Universidad Juarez Autonoma de Tabasco, Cunduacan, Tabasco, CP 86690, AP 24 (Mexico); Flores, M. [Departamento de Ingenieria de Proyectos, CUCEI, Universidad de Guadalajara, AP 307, Zapopan Jal 45101 (Mexico); Escamilla, R., E-mail: rauleg@servidor.unam.m [Instituto de Investigaciones en Materiales, Universidad Nacional Autonoma de Mexico, 04510 Mexico D.F. (Mexico)

    2010-05-01

    X-ray photoelectron spectroscopy (XPS) and ultraviolet photoelectron spectroscopy (UPS) were used to investigate the binding energies and valence band for ZrB{sub 2} and ZrB{sub 12}. The Zr 3d and B 1s core levels were identified. The Zr 3d core level shows a spin-orbit split 3d{sub 5/2} and 3d{sub 3/2} while that for B 1s core level exhibited a single symmetric peak, these being typical of zirconium and boride signals. Comparing the Zr 3d and B 1s core levels with metallic Zr, B{sub 2}O{sub 3} and ZrO{sub 2} reference materials only a negative chemical shift for Zr 3d associated to ZrB{sub 2} was observed, which suggests that the charge transfer model based on the concept of electronegativity was not applicable to explain the superconductivity in the ZrB{sub 12} sample. The measured valence band using UPS is consistent with the band-structure calculations indicating a higher density of states (DOS) at E{sub F} for ZrB{sub 12} respect to ZrB{sub 2}. Finally, we found that the weak mixed B-p and Zr-d states for ZrB{sub 12} is crucial for the superconductivity due to the state population increased the DOS at the E{sub F}.

  9. An implementation of core level spectroscopies in a real space Projector Augmented Wave density functional theory code

    DEFF Research Database (Denmark)

    Ljungberg, M.P.; Mortensen, Jens Jørgen; Pettersson, L.G.M.

    2011-01-01

    We describe the implementation of K-shell core level spectroscopies (X-ray absorption (XAS), X-ray emission (XES), and X-ray photoemission (XPS)) in the real-space-grid-based Projector Augmented Wave (PAW) GPAW code. The implementation for XAS is based on the Haydock recursion method avoiding com...

  10. Usage Level of Non-Farsi Periodicals within Arts Library and Determination of Core Journals

    Directory of Open Access Journals (Sweden)

    Saadat Ahmadzadeh

    2007-07-01

    Full Text Available The present study was conducted to determine the core non-farsi periodicals within the library of the Faculty of Fine Arts in University of Tehran. 125 non-farsi journal subscriptions for 2004 were selected. 150 graduate students and 50 faculty members were surveyed. Bibliometric method along with statistical analysis were employed to determine the core journals. Journals within any given field were ranked by both students and the faculty. Core journals were matched against these ranked journals. Given the foreign currency constraints, acquisition of titles really needed would maximize the existing capabilities as well as preventing unnecessary purchases. The existing periodicals provided only 49.3% of research queries. Students and faculty usage rate of images contained within these journal was 70.6% while only 29.4% was dedicated to printed articles

  11. Direct imaging the upconversion nanocrystal core/shell structure at the subnanometer level: shell thickness dependence in upconverting optical properties.

    Science.gov (United States)

    Zhang, Fan; Che, Renchao; Li, Xiaomin; Yao, Chi; Yang, Jianping; Shen, Dengke; Hu, Pan; Li, Wei; Zhao, Dongyuan

    2012-06-13

    Lanthanide-doped upconversion nanoparticles have shown considerable promise in solid-state lasers, three-dimensional flat-panel displays, and solar cells and especially biological labeling and imaging. It has been demonstrated extensively that the epitaxial coating of upconversion (UC) core crystals with a lattice-matched shell can passivate the core and enhance the overall upconversion emission intensity of the materials. However, there are few papers that report a precise link between the shell thickness of core/shell nanoparticles and their optical properties. This is mainly because rare earth fluoride upconversion core/shell structures have only been inferred from indirect measurements to date. Herein, a reproducible method to grow a hexagonal NaGdF(4) shell on NaYF(4):Yb,Er nanocrystals with monolayer control thickness is demonstrated for the first time. On the basis of the cryo-transmission electron microscopy, rigorous electron energy loss spectroscopy, and high-angle annular dark-field investigations on the core/shell structure under a low operation temperature (96 K), direct imaging the NaYF(4):Yb,Er@NaGdF(4) nanocrystal core/shell structure at the subnanometer level was realized for the first time. Furthermore, a strong linear link between the NaGdF(4) shell thickness and the optical response of the hexagonal NaYF(4):Yb,Er@NaGdF(4) core/shell nanocrystals has been established. During the epitaxial growth of the NaGdF(4) shell layer by layer, surface defects of the nanocrystals can be gradually passivated by the homogeneous shell deposition process, which results in the obvious enhancement in overall UC emission intensity and lifetime and is more resistant to quenching by water molecules.

  12. Microstructure-dependent mechanical properties of electrospun core-shell scaffolds at multi-scale levels.

    Science.gov (United States)

    Horner, Christopher B; Ico, Gerardo; Johnson, Jed; Zhao, Yi; Nam, Jin

    2016-06-01

    Mechanical factors among many physiochemical properties of scaffolds for stem cell-based tissue engineering significantly affect tissue morphogenesis by controlling stem cell behaviors including proliferation and phenotype-specific differentiation. Core-shell electrospinning provides a unique opportunity to control mechanical properties of scaffolds independent of surface chemistry, rendering a greater freedom to tailor design for specific applications. In this study, we synthesized electrospun core-shell scaffolds having different core composition and/or core-to-shell dimensional ratios. Two independent biocompatible polymer systems, polyetherketoneketone (PEKK) and gelatin as the core materials while maintaining the shell polymer with polycaprolactone (PCL), were utilized. The mechanics of such scaffolds was analyzed at the microscale and macroscales to determine the potential implications it may hold for cell-material and tissue-material interactions. The mechanical properties of individual core-shell fibers were controlled by core-shell composition and structure. The individual fiber modulus correlated with the increase in percent core size ranging from 0.55±0.10GPa to 1.74±0.22GPa and 0.48±0.12GPa to 1.53±0.12GPa for the PEKK-PCL and gelatin-PCL fibers, respectively. More importantly, it was demonstrated that mechanical properties of the scaffolds at the macroscale were dominantly determined by porosity under compression. The increase of scaffold porosity from 70.2%±1.0% to 93.2%±0.5% by increasing the core size in the PEKK-PCL scaffold resulted in the decrease of the compressive elastic modulus from 227.67±20.39kPa to 14.55±1.43kPa while a greater changes in the porosity of gelatin-PCL scaffold from 54.5%±4.2% to 89.6%±0.4% resulted in the compressive elastic modulus change from 484.01±30.18kPa to 17.57±1.40kPa. On the other hand, the biphasic behaviors under tensile mechanical loading result in a range from a minimum of 5.42±1.05MPa to a maximum

  13. Species-level core oral bacteriome identified by 16S rRNA pyrosequencing in a healthy young Arab population

    Directory of Open Access Journals (Sweden)

    Nezar Noor Al-hebshi

    2016-05-01

    Full Text Available Background: Reports on the composition of oral bacteriome in Arabs are lacking. In addition, the majority of previous studies on other ethnic groups have been limited by low-resolution taxonomic assignment of next-generation sequencing reads. Furthermore, there has been a conflict about the existence of a ‘core’ bacteriome. Objective: The objective of this study was to characterize the healthy core oral bacteriome in a young Arab population at the species level. Methods: Oral rinse DNA samples obtained from 12 stringently selected healthy young subjects of Arab origin were pyrosequenced (454's FLX chemistry for the bacterial 16S V1–V3 hypervariable region at an average depth of 11,500 reads. High-quality, non-chimeric reads ≥380 bp were classified to the species level using the recently described, prioritized, multistage assignment algorithm. A core bacteriome was defined as taxa present in at least 11 samples. The Chao2, abundance-based coverage estimator (ACE, and Shannon indices were computed to assess species richness and diversity. Results: Overall, 557 species-level taxa (211±42 per subject were identified, representing 122 genera and 13 phyla. The core bacteriome comprised 55 species-level taxa belonging to 30 genera and 7 phyla, namely Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, Fusobacteria, Saccharibacteria, and SR1. The core species constituted between 67 and 87% of the individual bacteriomes. However, the abundances differed by up to three orders of magnitude among the study subjects. On average, Streptococcus mitis, Rothia mucilaginosa, Haemophilus parainfluenzae, Neisseria flavescence/subflava group, Prevotella melaninogenica, and Veillonella parvula group were the most abundant. Streptococcus sp. C300, a taxon never reported in the oral cavity, was identified as a core species. Species richness was estimated at 586 (Chao2 and 614 (ACE species, whereas diversity (Shannon index averaged at 3.99. Conclusions

  14. Core Journals in library and information science: Measuring the level of specialization over time

    DEFF Research Database (Denmark)

    Nicolaisen, Jeppe; Frandsen, Tove Faber

    2013-01-01

    years. The method is applied to a selection of core journals in library and information science (1990-2012). The reference lists of each journal are compared year by year, and the percentage of re-citations is calculated by dividing the number of re-citations with the total number of citations each year......Introduction. Specialization in science is a process that occurs over time. The present paper presents a bibliometric method for measuring the degree of specialization over time. Methods. The method is based on bibliographic coupling, and counts the percentage of recitations given in subsequent...

  15. Ab initio molecular dynamics study of hydrogen cleavage by a Lewis base [tBu3P] and a Lewis acid [B(C6F5)3] at the mesoscopic level--dynamics in the solute-solvent molecular clusters.

    Science.gov (United States)

    Pu, Maoping; Privalov, Timofei

    2014-12-01

    With the help of state-of-the-art ab initio molecular dynamics methods, we investigated the reaction pathway of the {tBu3 P + H2 + B(C6 F5 )3 } system at the mesoscopic level. It is shown that: i) the onset of H2 activation is at much larger boron⋅⋅⋅phosphorus distances than previously thought; ii) the system evolves to the product in a roaming-like fashion because of quasi-periodic nuclear motion along the asymmetric normal mode of P⋅⋅⋅HH⋅⋅⋅B fragment; iii) transient configurations of a certain type are present despite structural interference from the solvent; iv) transient-state configurations with sub-picosecond lifetime have potentially interesting infrared activity in the organic solvent (toluene) as well as in the gas phase. The presented results should be helpful for future experimental and theoretical studies of frustrated Lewis pair (FLP) activity.

  16. Determination of the level of water in the core of reactors PWR using neutron detectors signal ex core; Determinacion del nivel del agua del nucleo de reactores PWR usando la senal de detectores neutronicos excore

    Energy Technology Data Exchange (ETDEWEB)

    Bernal, A.; Abarca, A.; Miro, R.; Verdu, G.

    2014-07-01

    The level of water from the core provides relevant information of the neutronic and thermal hydraulic of the reactor as the power, k EFF and cooling capacity. In fact, this level monitoring can be used for prediction of LOCA and reduction of cooling that can cause damage to the core. There are several teams that measure a variety of parameters of the reactor, as opposed to the level of the water of the core. However, the detectors 'excore' measure fast neutrons which escape from the core and there are studies that demonstrate the existence of a relationship between them and the water level of the kernel due to the water shield. Therefore, a methodology has been developed to determine this relationship, using the Monte Carlo method using the MCNP code and apply variance reduction techniques based on the attached flow that is obtained using the method of discrete ordinates using code TORT. (Author)

  17. Yes we can! Eliminating health disparities as part of the core business of nursing on a global level.

    Science.gov (United States)

    Villeneuve, Michael J

    2008-11-01

    Nurses in the 21st century are being called to rise to new levels of practice, including a more influential leadership at senior levels of policy development. Decades of research, good will, and a revolutionary civil rights movement have not resolved the world's staggering health outcome disparities. Nursing has a solution: Many of the most troubling disparities are amenable to effective intervention by the world's nurses through their clinical and policy work. The author challenges nurses to imagine the impact on global health if the elimination of disparities is the core goal of nursing for the 21st century. Moving from individuals and communities to systems levels, nurses must be versed in a range of system-level vital signs that affect policy development including economics, demographics, and access to care. Setting our sights on the elimination of health disparities offers a rallying point around which nursing can coalesce and set human health on a new and more equitable course.

  18. Magnetic Properties of Quantized Vortices in Neutron $^3P_2$ Superfluids in Neutron Stars

    CERN Document Server

    Masuda, Kota

    2015-01-01

    We discuss quantized vortices in neutron $^3P_2$ superfluids, which are believed to realize in high density neutron matter such as neutron stars. By using the Ginzburg-Landau free energy for $^3P_2$ superfluids, we determine the ground state in the absence and presence of the external magnetic field, and numerically construct $^3P_2$ quantized vortices in the absence and presence of the external magnetic field along the vortex axis (poloidal) or angular direction (toroidal). We find in certain situations the spontaneous magnetization of the vortex core, whose typical magnitude is about $10^{7-8}$ Gauss, but the net magnetic field in a neutron star is negligible because of the ratio of the vortex core size $\\sim 10$fm and the intervortex distance $\\sim 10^{-6}$m in a vortex lattice.

  19. ICL-induced miR139-3p and miR199a-3p have opposite roles in hematopoietic cell expansion and leukemic transformation.

    Science.gov (United States)

    Alemdehy, Mir Farshid; Haanstra, Jurgen R; de Looper, Hans W J; van Strien, Paulina M H; Verhagen-Oldenampsen, Judith; Caljouw, Yvette; Sanders, Mathijs A; Hoogenboezem, Remco; de Ru, Arnoud H; Janssen, George M C; Smetsers, Stephanie E; Bierings, Marc B; van Veelen, Peter A; von Lindern, Marieke; Touw, Ivo P; Erkeland, Stefan J

    2015-06-18

    Interstrand crosslinks (ICLs) are toxic DNA lesions that cause severe genomic damage during replication, especially in Fanconi anemia pathway-deficient cells. This results in progressive bone marrow failure and predisposes to acute myeloid leukemia (AML). The molecular mechanisms responsible for these defects are largely unknown. Using Ercc1-deficient mice, we show that Trp53 is responsible for ICL-induced bone marrow failure and that loss of Trp53 is leukemogenic in this model. In addition, Ercc1-deficient myeloid progenitors gain elevated levels of miR-139-3p and miR-199a-3p with age. These microRNAs exert opposite effects on hematopoiesis. Ectopic expression of miR-139-3p strongly inhibited proliferation of myeloid progenitors, whereas inhibition of miR-139-3p activity restored defective proliferation of Ercc1-deficient progenitors. Conversely, the inhibition of miR-199a-3p functions aggravated the myeloid proliferation defect in the Ercc1-deficient model, whereas its enforced expression enhanced proliferation of progenitors. Importantly, miR-199a-3p caused AML in a pre-leukemic mouse model, supporting its role as an onco-microRNA. Target genes include HuR for miR-139-3p and Prdx6, Runx1, and Suz12 for miR-199a-3p. The latter genes have previously been implicated as tumor suppressors in de novo and secondary AML. These findings show that, in addition to TRP53-controlled mechanisms, miR-139-3p and miR-199a-3p are involved in the defective hematopoietic function of ICL-repair deficient myeloid progenitors.

  20. Tautomerism in 5-aminotetrazole investigated by core-level photoelectron spectroscopy and {Delta}SCF calculations

    Energy Technology Data Exchange (ETDEWEB)

    Pinto, R.M., E-mail: ruipinto@fct.unl.pt [CFA, Centro de Fisica Atomica, Departamento de Fisica, Faculdade de Ciencias e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica (Portugal); Dias, A.A. [CFA, Centro de Fisica Atomica, Departamento de Fisica, Faculdade de Ciencias e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica (Portugal); Coreno, M. [CNR-IMIP, Montelibretti, Rome I-00016 (Italy); Simone, M. de [CNR-IOM, Laboratorio TASC, 34149 Trieste (Italy); Giuliano, B.M. [Departamento de Quimica da Universidade de Coimbra, 3004-535 Coimbra (Portugal); Santos, J.P.; Costa, M.L. [CFA, Centro de Fisica Atomica, Departamento de Fisica, Faculdade de Ciencias e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica (Portugal)

    2012-03-15

    Highlights: Black-Right-Pointing-Pointer High-resolution XPS of 5-aminotetrazole reveals different tautomers. Black-Right-Pointing-Pointer 5ATZ exists mainly in the 2H-form. Black-Right-Pointing-Pointer Results obtained with DSCF are in good agreement with the observed binding energies. - Abstract: The C 1s and N 1s photoelectron spectra of gas-phase 5-aminotetrazole (5ATZ) were recorded using synchrotron radiation, with the aim of evaluating 1H/2H tautomer population ratios. The core-electron binding energies (CEBEs) were estimated from computational results, using the delta self-consistent-field ({Delta}SCF) approach. Simulated spectra were generated using these CEBEs and the results from GAUSSIAN-n (Gn, n = 1, 2 and 3) and Complete Basis Set (CBS-4M and CBS-Q) methods. Results reveal the almost exclusive predominance of the 2H-tautomer, with a 1H/2H ratio of ca. 0.12/0.88, taken from a gross analysis of the XPS C 1s spectrum, recorded at 365 K.

  1. Coordination-resolved local bond strain and 3p energy entrapment of K atomic clusters and K(1 1 0) skin

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Ting; Bo, Maolin; Guo, Yongling [Key Laboratory of Low-Dimensional Materials and Application Technologies (Ministry of Education), Faculty of Materials Science and Engineering, Xiangtan University, Hunan 411105 (China); Hunan Provincial Key Laboratory of Thin Film Materials and Devices, Faculty of Materials Science and Engineering, Xiangtan University, Hunan 411105 (China); Chen, Hefeng [United Superconductive Institution, Shanghai Jiaotong University, Shanghai 200240 (China); Wang, Yan [School of Information and Electronic Engineering, Hunan University of Science and Technology, Hunan 411201 (China); Huang, Yongli, E-mail: huangyongli@xtu.edu.cn [Key Laboratory of Low-Dimensional Materials and Application Technologies (Ministry of Education), Faculty of Materials Science and Engineering, Xiangtan University, Hunan 411105 (China); Hunan Provincial Key Laboratory of Thin Film Materials and Devices, Faculty of Materials Science and Engineering, Xiangtan University, Hunan 411105 (China); Sun, Chang Q., E-mail: ecqsun@ntu.edu.sg [Key Laboratory of Low-Dimensional Materials and Application Technologies (Ministry of Education), Faculty of Materials Science and Engineering, Xiangtan University, Hunan 411105 (China); Hunan Provincial Key Laboratory of Thin Film Materials and Devices, Faculty of Materials Science and Engineering, Xiangtan University, Hunan 411105 (China); School of Information and Electronic Engineering, Hunan University of Science and Technology, Hunan 411201 (China); NOVITAS, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore 639798 (Singapore)

    2015-09-15

    Graphical abstract: - Highlights: • Coordination environment resolves electron binding-energy shift of K{sub 44}, K{sub 46}, K{sub 55} clusters. • Predict the effective coordination number of K nanoclusters when we get the atomic number N. • Atomic under coordination shortens the local bonds and entrapment. • XPS derives core level of an isolated atom and its bulk shift. - Abstract: We have examined the atomic coordination effect on the local bond strain and the 3p core-level shift of K(1 1 0) skin and nanoclusters using a combination of the bond order–length–strength correlation notion, tight-binding approach, density functional theory calculations, and photoelectron spectroscopy measurements. It turns out that: (i) the 3p core-level shifts from 15.595 ± 0.003 eV for an isolated K atom by 2.758 eV to the bulk value of 18.353 eV; (ii) the effective atomic coordination number reduces from the bulk value of 12 to 3.93 for the first layer and to 5.81 for the second layer of K(1 1 0) skin associated with the local lattice strain of 12.76%, a binding energy density 72.67%, and atomic cohesive energy −62.46% for the skin; and (iii) K cluster size reduction lowers the effective atomic coordination number and enhances further the skin electronic attribution. Results have revealed that the 3p core-level shifts of K(1 1 0) and nanoclusters originate from perturbation of the Hamiltonian by under-coordination induced charge densification and quantum entrapment.

  2. Photoelectron spectroscopy at a free-electron laser. Investigation of space-charge effects in angle-resolved and core-level spectroscopy and realizaton of a time-resolved core-level photoemission experiment

    Energy Technology Data Exchange (ETDEWEB)

    Marczynski-Buehlow, Martin

    2012-01-30

    The free-electron laser (FEL) in Hamburg (FLASH) is a very interesting light source with which to perform photoelectron spectroscopy (PES) experiments. Its special characteristics include highly intense photon pulses (up to 100 J/pulse), a photon energy range of 30 eV to 1500 eV, transverse coherence as well as pulse durations of some ten femtoseconds. Especially in terms of time-resolved PES (TRPES), the deeper lying core levels can be reached with photon energies up to 1500 eV with acceptable intensity now and, therefore, element-specific, time-resolved core-level PES (XPS) is feasible at FLASH. During the work of this thesis various experimental setups were constructed in order to realize angle-resolved (ARPES), core-level (XPS) as well as time-resolved PES experiments at the plane grating monochromator beamline PG2 at FLASH. Existing as well as newly developed systems for online monitoring of FEL pulse intensities and generating spatial and temporal overlap of FEL and optical laser pulses for time-resolved experiments are successfully integrated into the experimental setup for PES. In order to understand space-charge effects (SCEs) in PES and, therefore, being able to handle those effects in future experiments using highly intense and pulsed photon sources, the origin of energetic broadenings and shifts in photoelectron spectra are studied by means of a molecular dynamic N-body simulation using a modified Treecode Algorithm for sufficiently fast and accurate calculations. It turned out that the most influencing parameter is the ''linear electron density'' - the ratio of the number of photoelectrons to the diameter of the illuminated spot on the sample. Furthermore, the simulations could reproduce the observations described in the literature fairly well. Some rules of thumb for XPS and ARPES measurements could be deduced from the simulations. Experimentally, SCEs are investigated by means of ARPES as well as XPS measurements as a function of

  3. Spectra and oscillator strengths of 3p63d9-3p53d10 and 3p63d9-3p63d84p transitions for cobalt-like Sn23+ ion

    Institute of Scientific and Technical Information of China (English)

    Chen Ming-Lun; Yu Xiao-Guang

    2009-01-01

    This paper calculates the spectra and oscillator strengths for highly ionized cobalt-like Sn23+ ions 3p63d9-3p53d10,3p63d9 - 3p63d84p transitions by using a multi-configuration self-consistent field method program together with the proposed fitting formula. The calculations have a good agreement with observations.

  4. Precore/basal core promoter mutants and hepatitis B viral DNA levels as predictors for liver deaths and hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    Myron J Tong; Lawrence M Blatt; Jia-Horng Kao; Jason Tzuying Cheng; William G Corey

    2006-01-01

    AIM: To conduct a retrospective study in 400 chronic hepatitis B patients in order to identify hepatitis B viral factors associated with complications of liver disease or development of hepatocellular carcinoma.METHODS: The mean follow-up time was 83.6 ± 39.6mo. Alpha-fetoprotein test and abdominal ultrasound were used for cancer surveillance. Hepatitis B basal core promoter mutants, precore mutants, genotypes,hepatitis B viral DNA (HBV DNA) level and hepatitis B e antigen (HBeAg) were measured. Univariate analysis and logistic regression were used to assess odds ratios for viral factors related to liver deaths and hepatocellular carcinoma development.RESULTS: During follow-up, 38 patients had liver deaths not related to hepatocellular carcinoma. On multivariate analysis, older age [odds ratio: 95.74 (12.13-891.31);P < 0.0001], male sex [odds ratio: 7.61 (2.20-47.95);P = 0.006], and higher log10 HBV DNA [odds ratio:4.69 (1.16-20.43); P < 0.0001] were independently predictive for these liver related deaths. Also, 31 patients developed hepatocellular carcinoma. Multivariate analysis showed that older age [odds ratio: 26.51 (2.36-381.47);P = 0.007], presence of precore mutants [odds ratio:4.23 (1.53-19.58); P = 0.02] and presence of basal core promoter mutants [odds ratio: 2.93 (1.24-7.57); P =0.02] were independent predictors for progression to hepatocellular carcinoma.CONCLUSION: Our results show that high levels of baseline serum HBV DNA are associated with nonhepatocellular carcinoma-related deaths of liver failure,while genetic mutations in the basal core promoter and precore regions are predictive for development of hepatocellular carcinoma.

  5. Core levels, valence band structure and unoccupied states of clean InN surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Himmerlich, Marcel; Eisenhardt, Anja; Schaefer, Juergen A.; Krischok, Stefan [Institut fuer Physik and Institut fuer Mikro- und Nanotechnologien, TU Ilmenau (Germany)

    2008-07-01

    In this study we used a surface analytics system directly connected to a MBE growth module to study the surface properties of thin InN films. The samples were prepared by plasma assisted molecular beam epitaxy on GaN/Al{sub 2}O{sub 3}(0001) templates and exhibited a 2 x 2 reconstruction after growth. The prepared samples were analysed by photoelectron spectroscopy as well as electron energy loss spectroscopy (EELS). For the occupied states, a very good agreement to available theoretical calculations is found. Although, the valence band maximum is located at 1.6 eV, indicating strong downward band bending of {proportional_to}0.9 eV, photoemission is detected up to E{sub F}. This indicates that the Fermi level is pinned above the conduction band minimum, as recently predicted. The spin-orbit splitting of the In 4d level at 17.8 eV could be resolved using He II radiation. Furthermore, from the fine structure of the secondary electron cascade peak we extract the energy of different unoccupied states 0 eV to 9 eV above the vacuum level. These measurements enable us to identify features in the InN EELS spectra, with a loss energy larger than 16 eV, as interband transitions from the In 4d level.

  6. The core and conserved role of MAL is homeostatic regulation of actin levels.

    Science.gov (United States)

    Salvany, Lara; Muller, Julius; Guccione, Ernesto; Rørth, Pernille

    2014-05-15

    The transcription cofactor MAL is regulated by free actin levels and thus by actin dynamics. MAL, together with its DNA-binding partner, SRF, is required for invasive cell migration and in experimental metastasis. Although MAL/SRF has many targets, we provide genetic evidence in both Drosophila and human cellular models that actin is the key target that must be regulated by MAL/SRF for invasive cell migration. By regulating MAL/SRF activity, actin protein feeds back on production of actin mRNA to ensure sufficient supply of actin. This constitutes a dedicated homeostatic feedback system that provides a foundation for cellular actin dynamics.

  7. Core-level spectroscopy investigation of the Mo{sub 0.75}Re{sub 0.25}(100) surface

    Energy Technology Data Exchange (ETDEWEB)

    Lyman, P.F.; Zehner, D.M.

    1993-10-01

    Preferential surface segregation in the Mo{sub 0.75}(100) surface region was investigated using high-resolution core-level spectroscopy with synchrotron radiation. The magnitude and direction of the surface core-level shifts observed in this study can be qualitatively understood by comparison to W and Mo core-level shifts. Measured core-level intensities are found to be consistent with the segregation of Mo to the surface of the alloy, with an enrichment of Re in the second layer (as found in previous investigations). It is inferred that both Tc and Os will segregate to the Mo{sub 0.75}Re{sub 0.25}(100) surface.

  8. Radiological consequence assessments of degraded core accident scenarios derived from a generic Level 2 PSA of a BWR

    Energy Technology Data Exchange (ETDEWEB)

    Homma, Toshimitsu; Ishikawa, Jun; Tomita, Kenichi; Muramatsu, Ken [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    2000-12-01

    The radiological consequence assessments have been made of postulated core damage accidents with source terms derived from a generic Level 2 PSA of a BWR carried out by the Japan Atomic Energy Research Institute (JAERI). The source terms used were for the five core damage accident sequences with the drywell and wetwell failure cases, the release control case by venting of the containment and the accident termination case by the containment spray. The radiological consequences have been assessed for individual dose, collective dose, individual risk of early health effects and individual risk of late health effects by a probabilistic accident consequence assessment code, OSCAAR developed in JAERI. Following conclusions were obtained for the assumed source terms. In case of the over pressure failures of the primary containment vessel, the early fatalities can be mitigated through the implementation of early countermeasures, and the late cancer fatalities remains small. For the release control and accident termination cases, the individual and collective doses to the public can be reduced without any countermeasures due to the release reduction of the volatile radionuclides such as iodine and cesium. (author)

  9. On sulfur core level binding energies in thiol self-assembly and alternative adsorption sites: An experimental and theoretical study

    Energy Technology Data Exchange (ETDEWEB)

    Jia, Juanjuan [Institut des Sciences Moléculaires d’Orsay, Université-Paris Sud, 91405 Orsay (France); CNRS, UMR 8214, Institut des Sciences Moléculaires d’Orsay, Orsay ISMO, Bâtiment 351, Université Paris Sud, 91405 Orsay (France); Kara, Abdelkader, E-mail: abdelkader.kara@ucf.edu, E-mail: vladimir.esaulov@u-psud.fr [Department of Physics, University of Central Florida, Orlando, Florida 32816 (United States); Pasquali, Luca [Dipartimento di Ingegneria “E. Ferrari,” Università di Modena e Reggio Emilia, Via Vignolese 905, 41125 Modena (Italy); IOM-CNR, s.s. 14, Km. 163.5 in AREA Science Park, 34149 Basovizza, Trieste (Italy); Department of Physics, University of Johannesburg, P.O. Box 524, Auckland Park 2006 (South Africa); Bendounan, Azzedine; Sirotti, Fausto [Synchrotron SOLEIL, L’Orme des Merisiers, Saint-Aubin, BP 48, F-91192 Gif-sur-Yvette Cedex (France); Esaulov, Vladimir A., E-mail: abdelkader.kara@ucf.edu, E-mail: vladimir.esaulov@u-psud.fr [Institut des Sciences Moléculaires d’Orsay, Université-Paris Sud, 91405 Orsay (France); CNRS, UMR 8214, Institut des Sciences Moléculaires d’Orsay, Orsay ISMO, Bâtiment 351, Université Paris Sud, 91405 Orsay (France); IOM-CNR, s.s. 14, Km. 163.5 in AREA Science Park, 34149 Basovizza, Trieste (Italy)

    2015-09-14

    Characteristic core level binding energies (CLBEs) are regularly used to infer the modes of molecular adsorption: orientation, organization, and dissociation processes. Here, we focus on a largely debated situation regarding CLBEs in the case of chalcogen atom bearing molecules. For a thiol, this concerns the case when the CLBE of a thiolate sulfur at an adsorption site can be interpreted alternatively as due to atomic adsorption of a S atom, resulting from dissociation. Results of an investigation of the characteristics of thiol self-assembled monolayers (SAMs) obtained by vacuum evaporative adsorption are presented along with core level binding energy calculations. Thiol ended SAMs of 1,4-benzenedimethanethiol (BDMT) obtained by evaporation on Au display an unconventional CLBE structure at about 161.25 eV, which is close to a known CLBE of a S atom on Au. Adsorption and CLBE calculations for sulfur atoms and BDMT molecules are reported and allow delineating trends as a function of chemisorption on hollow, bridge, and atop sites and including the presence of adatoms. These calculations suggest that the 161.25 eV peak is due to an alternative adsorption site, which could be associated to an atop configuration. Therefore, this may be an alternative interpretation, different from the one involving the adsorption of atomic sulfur resulting from the dissociation process of the S–C bond. Calculated differences in S(2p) CLBEs for free BDMT molecules, SH group sulfur on top of the SAM, and disulfide are also reported to clarify possible errors in assignments.

  10. Unified interpretation of Hund's first and second rules for 2p and 3p atoms.

    Science.gov (United States)

    Oyamada, Takayuki; Hongo, Kenta; Kawazoe, Yoshiyuki; Yasuhara, Hiroshi

    2010-10-28

    A unified interpretation of Hund's first and second rules for 2p (C, N, O) and 3p (Si, P, S) atoms is given by Hartree-Fock (HF) and multiconfiguration Hartree-Fock (MCHF) methods. Both methods exactly satisfy the virial theorem, in principle, which enables one to analyze individual components of the total energy E(=T+V(en)+V(ee)), where T, V(en), and V(ee) are the kinetic, the electron-nucleus attraction, and the electron-electron repulsion energies, respectively. The correct interpretation for each of the two rules can only be achieved under the condition of the virial theorem 2T+V=0 by investigating how V(en) and V(ee) interplay to attain the lower total potential energy V(=V(en)+V(ee)). The stabilization of the more stable states for all the 2p and 3p atoms is ascribed to a greater V(en) that is caused by contraction of the valence orbitals accompanied with slight expansion of the core orbitals. The contraction of the valence orbitals for the two rules is a consequence of reducing the Hartree screening of the nucleus at short interelectronic distances. The reduced screening in the first rule is due to a greater amount of Fermi hole contributions in the state with the highest total spin-angular momentum S. The reduced screening in the second rule is due to the fact that two valence electrons are more likely to be on opposite sides of the nucleus in the state with the highest total orbital-angular momentum L. For each of the two rules, the inclusion of correlation does not qualitatively change the HF interpretation, but HF overestimates the energy difference ∣ΔE∣ between two levels being compared. The magnitude of the correlation energy is significantly larger for the lower L states than for the higher L states since two valence electrons in the lower L states are less likely to be on opposite sides of the nucleus. The MCHF evaluation of ∣ΔE∣ is in excellent agreement with experiment. The present HF and MCHF calculations demonstrate the above statements

  11. Elevation of circulating miR-210-3p in high-altitude hypoxic environment

    Directory of Open Access Journals (Sweden)

    Yan eYan

    2016-03-01

    Full Text Available Background: The induction of miR-210-3p, a master hypoxamir, is a consistent feature of the hypoxic response in both normal and malignant cells. However, whether miR-210-3p acts as a circulating factor in response to a hypoxic environment remains unknown. The current study aimed to examine the effect of a high-altitude hypoxic environment on circulating miR-210-3p.Methods: We examined and compared the levels of miR-210-3p using TaqMan-based qRT-PCR in both peripheral blood cells and plasma from 84 ethnic Chinese Tibetans residing at 3560 m, 46 newly arrived migrant Han Chinese (Tibet Han and 82 Han Chinese residing at 8.9 m (Nanjing Han. Furthermore, we analyzed the correlations of miR-210-3p with hematological indices. Results: The relative concentrations of miR-210-3p to internal reference U6 in blood cells were significantly higher in the Tibet Han group (1.01±0.11, P<0.001 and in the Tibetan group (1.17±0.09, P<0.001 than in the Nanjing Han group (0.51±0.04. The absolute concentrations of plasma miR-210-3p were also markedly elevated in the Tibet Han group (503.54±42.95 fmol/L, P=0.004 and in the Tibetan group (557.78±39.84 fmol/L, P<0.001 compared to the Nanjing Han group (358.39±16.16 fmol/L. However, in both blood cells and plasma, miR-210-3p levels were not significantly different between the Tibet Han group and the Tibetan group (P=0.280, P=0.620, respectively. Plasma miR-210-3p concentrations were positively correlated with miR-210-3p levels in blood cells (r=0.192, P=0.005. Furthermore, miR-210-3p levels in both blood cells and plasma showed strong positive correlations with red blood cell counts and hemoglobin and hematocrit values. Conclusion: These data demonstrated, for the first time, that miR-210-3p might act as a circulating factor in response to hypoxic environments and could be associated with human adaptation to life at high altitudes.

  12. Evolution of a system sensitive to stochastic noise: P3.p cell fate in Caenorhabditis.

    Science.gov (United States)

    Pénigault, Jean-Baptiste; Félix, Marie-Anne

    2011-09-15

    The C. elegans cell lineage is overall invariant. One rare instance of variability concerns P3.p, the most anterior vulva precursor cell, which may either fuse with the epidermis without dividing, or remain competent to form vulval tissue and divide. Here we examine the evolutionary properties of this stochastic variation in P3.p fate. In the Caenorhabditis genus, high P3.p competence is ancestral and reduction in P3.p competence and division frequency occurred in C. sp. 14 and in a clade of nine species. Within this clade, the frequency of P3.p division further varies within and among species, being intermediate in C. elegans and low in C. briggsae. P3.p fate frequency is sensitive to random mutation accumulation, suggesting that this trait may evolve rapidly because of its sensitivity to mutational impact. P3.p fate depends on LIN-39/Hox5 expression and we find that the peak of LIN-39/Hox5 protein level is displaced posteriorly in C. briggsae compared to C. elegans. However, P3.p fate specification is most sensitive to the dose of EGL-20 and CWN-1, two Wnts that are secreted in a long-range gradient from the posterior end of C. elegans larvae (accompanying article). A half-dose of either of these Wnts is sufficient to affect division frequency in C. elegans N2 to levels similar to those in C. briggsae. Symmetrically, we show that an increase in Wnt dose rescues anterior competence in C. briggsae. We propose that evolutionary variation in the concentration or interpretation of the long-range Wnt gradient may be involved in the rapid evolution of P3.p fate in Caenorhabditis.

  13. Photoionization cross sections of the excited 3s3p 3Po state for atomic Mg

    Science.gov (United States)

    Wang, Guoli; Wan, Jianjie; Zhou, Xiaoxin

    2017-01-01

    The photoionization cross sections of the excited levels (3s3p 0,1,2,o 3P) of atomic Mg have been studied theoretically using both the nonrelativistic and fully relativistic R-matrix method. For the threshold cross sections, as previous nonrelativistic studies, present calculations show significant differences (a factor of 3) from former experimental values. Large discrepancies with experiment calls for additional measurements of the photoionization cross sections from the excited states of Mg.

  14. Late Maastrichtian-Early Paleocene sea level and climate changes in the Antioch Church Core (Alabama, Gulf of Mexico margin, USA): A multi-proxy approach

    OpenAIRE

    Schulte, Peter; Speijer, Robert

    2009-01-01

    The Antioch Church core from central Alabama, spanning the Cretaceous-Paleogene (K-P) boundary, was investigated by a multi-proxy approach to study paleoenvironmental and sea level hanges within the wellconstrained sequence stratigraphic setting of the Gulf of Mexico margin. The Antioch Church core comprises the Maastrichtian calcareous nannoplankton Zone CC25 and the Danian Zones NP1 to NP4 corresponding to the Maastrichtian planktonic foraminifera Zones CF3 and the Danian Zones P1a to P2....

  15. In-fiber liquid-level probe based on Michelson interferometer via dual-mode elliptical multilayer-core fiber

    Science.gov (United States)

    Liang, Xiao; Ren, Guobin; Li, Yang; Liu, Zhibo; Wei, Huai; Jian, Shuisheng

    2016-07-01

    An in-fiber liquid-level probe fabricated from homemade dual-mode elliptical multilayer-core fiber (EMCF) was proposed and experimentally demonstrated. The proposed sensor simply consists of a segment of the EMCF with one end coated with silver film, and a Michelson interferometer is roughly established when light from single-mode fiber incident from the other end. The detected interference patterns, rather clean due to the few-mode property, shift as the liquid level due to strong interaction between high-order modes and measurands through evanescent waves. Both the propagation characteristics and operation principle of such a sensor were demonstrated in detail, and sensitivities of 33.48, 43.35, and 48.93 pm/mm corresponding to liquid indices of 1.333, 1.353, and 1.373 were successfully achieved with a 50-mm EMCF probe, respectively. Moreover, the proposed sensor had the potential to discriminate measurand index after proper calibration.

  16. Role of miR-142-3p in the post-transcriptional regulation of the clock gene Bmal1 in the mouse SCN.

    Science.gov (United States)

    Shende, Vikram R; Neuendorff, Nichole; Earnest, David J

    2013-01-01

    MicroRNAs (miRNAs) are small non-coding RNAs that function as post-transcriptional modulators by regulating stability or translation of target mRNAs. Recent studies have implicated miRNAs in the regulation of mammalian circadian rhythms. To explore the role of miRNAs in the post-transcriptional modulation of core clock genes in the master circadian pacemaker, we examined miR-142-3p for evidence of circadian expression in the suprachiasmatic nuclei (SCN), regulation of its putative clock gene target Bmal1 via specific binding sites in the 3' UTR and overexpression-induced changes in the circadian rhythm of BMAL1 protein levels in SCN cells. In mice exposed to constant darkness (DD), miR-142-3p levels in the SCN were characterized by circadian rhythmicity with peak expression during early subjective day at CT 3. Mutagenesis studies indicate that two independent miRNA recognition elements located at nucleotides 1-7 and 335-357 contribute equally to miR-142-3p-induced repression of luciferase-reported Bmal1 3' UTR activity. Importantly, overexpression of miR-142-3p in immortalized SCN cells abolished circadian variation in endogenous BMAL1 protein levels in vitro. Collectively, our results suggest that miR-142-3p may play a role in the post-transcriptional modulation of Bmal1 and its oscillatory regulation in molecular feedback loops mediating SCN circadian function.

  17. Role of miR-142-3p in the post-transcriptional regulation of the clock gene Bmal1 in the mouse SCN.

    Directory of Open Access Journals (Sweden)

    Vikram R Shende

    Full Text Available MicroRNAs (miRNAs are small non-coding RNAs that function as post-transcriptional modulators by regulating stability or translation of target mRNAs. Recent studies have implicated miRNAs in the regulation of mammalian circadian rhythms. To explore the role of miRNAs in the post-transcriptional modulation of core clock genes in the master circadian pacemaker, we examined miR-142-3p for evidence of circadian expression in the suprachiasmatic nuclei (SCN, regulation of its putative clock gene target Bmal1 via specific binding sites in the 3' UTR and overexpression-induced changes in the circadian rhythm of BMAL1 protein levels in SCN cells. In mice exposed to constant darkness (DD, miR-142-3p levels in the SCN were characterized by circadian rhythmicity with peak expression during early subjective day at CT 3. Mutagenesis studies indicate that two independent miRNA recognition elements located at nucleotides 1-7 and 335-357 contribute equally to miR-142-3p-induced repression of luciferase-reported Bmal1 3' UTR activity. Importantly, overexpression of miR-142-3p in immortalized SCN cells abolished circadian variation in endogenous BMAL1 protein levels in vitro. Collectively, our results suggest that miR-142-3p may play a role in the post-transcriptional modulation of Bmal1 and its oscillatory regulation in molecular feedback loops mediating SCN circadian function.

  18. Quark structure of the X(3872) and χb(3P) resonances

    Science.gov (United States)

    Ferretti, J.; Galatà, G.; Santopinto, E.

    2014-09-01

    We discuss the nature of the χb(3P) and X(3872) mesons. Are the χb(3P)'s standard bb¯ mesons or bb¯ states with a significative continuum component? Is the X(3872) a cc¯ state with continuum coupling effects or a meson-meson molecule? To do that, we compare quark model and unquenched quark model results for the mass barycenter and splittings of the χb(3P) multiplet. Future and more precise experimental results will discriminate between the two interpretations. In the case of the X(3872), we interpret it as a cc¯ core plus higher Fock components due to the coupling to the meson-meson continuum, and thus we think that it is compatible with the meson χc1(2P), with JPC=1++. The JPC=1++ quantum numbers are in agreement with the experimental results found by the LHCb collaboration. In our view, the X(3872)'s mass is lower than the quark model's predictions because of self-energy shifts. We also provide an estimation of the open charm/bottom strong decay modes of the X(3872) and χb(3P) mesons, such as X(3872)→DD¯* and χb2(3P)→BB¯, and radiative transitions.

  19. Ce Core-Level Spectroscopy, and Magnetic and Electrical Transport Properties of Lightly Ce-Doped YCoO3

    Science.gov (United States)

    Kobayashi, Yoshihiko; Koike, Tsuyoshi; Okawa, Mario; Takayanagi, Ryohei; Takei, Shohei; Minohara, Makoto; Kobayashi, Masaki; Horiba, Koji; Kumigashira, Hiroshi; Yasui, Akira; Ikenaga, Eiji; Saitoh, Tomohiko; Asai, Kichizo

    2016-11-01

    We have investigated the Ce and Co core level spectroscopy, and the magnetic and electrical transport properties of lightly Ce-doped YCoO3. We have successfully synthesized single-phase Y1-xCexCoO3 for 0.0 ≤ x ≤ 0.1 by the sol-gel method. Hard X-ray photoelectron and X-ray absorption spectroscopy experiments reveal that the introduced Ce ions are tetravalent, which is considered to be the first case of electron doping into bulk trivalent Co oxides with perovskite RECoO3 (RE: rare-earth element or Y) caused by RE site substitution. The magnitude of the effective magnetic moment peff obtained from the temperature dependence of magnetic susceptibility χ(T) at higher temperatures is close to that for high-spin Co2+ introduced by the Ce doping, implying that the electrons doped into the Co site induce Co2+ with a high-spin state. For x = 0.1, ferromagnetic ordering is observed below about 7 K. Electrical transport properties such as resistivity and thermoelectric power show that negative electron-like carriers are introduced by Ce substitution.

  20. Where are $\\chi_{cJ}(3P)$?

    CERN Document Server

    Chen, Dian-Yong

    2016-01-01

    In the present work, we propose the $Y(4140)$ as the $\\chi_{c1}(3P)$ state by studying the $\\chi_{c1} \\pi^+ \\pi^-$ invariant mass spectrum of the $B\\to K \\chi_{c1} \\pi^+ \\pi^-$ process. In the $D\\bar{D}$ invariant mass spectrum of the $B\\to K D\\bar{D}$ process, we find a new resonance with the mass and width to be $ (4083.0 \\pm 5.0) $ and $ (24.1 \\pm 15.4) $ MeV, respectively, which could be a good candidate of the $\\chi_{c0}(3P)$ state. The theoretical investigations on the decay behaviors of the $\\chi_{cJ}(3P)$ in the present work support the assignments of the $Y(4140)$ and $Y(4080)$ as the $\\chi_{c1}(3P)$ and $\\chi_{c0}(3P)$ states, respectively. In addition, the $\\chi_{c2}(3P)$ state is predicted to be a very narrow state. The results in the present work could be tested by further experiments in the LHCb and forthcoming Belle II.

  1. In-fiber quasi-Michelson interferometer for liquid level measurement with a core-cladding-modes fiber end-face mirror

    Science.gov (United States)

    Rong, Qiangzhou; Qiao, Xueguang; Du, Yanying; Sun, Hao; Feng, Dingyi; Wang, Ruohui; Hu, Manli; Feng, Zhongyao

    2014-06-01

    An in-fiber quasi-Michelson interferometer (IFQMI) working on reflection is proposed and experimentally demonstrated for liquid level measurement. The device consists of a short piece of small-core fiber (SCF) followed by a standard single-mode fiber (SMF) where its end-face is terminated by a thick silver film. A well-defined interference pattern is obtained as the result of the fiber-core mismatch and core-cladding modes interference. The proposed device with a 30 mm pigtail SMF at a wavelength of 1555 nm presents a water level sensitivity of -68.3 pm/mm. Besides, the proposed device can also discriminate the refractive index (RI) of liquid measured. The IFQMI with 50 mm-long SMF provides a high RI sensitivity of -1200.61 (pm/mm)/RIU.

  2. A search at the millijansky level for milli-arcsecond cores in a complete sample of radio galaxies

    Science.gov (United States)

    Wehrle, A. E.; Preston, R. A.; Meier, D. L.; Gorenstein, M. V.; Shapiro, I. I.; Rogers, A. E. E.; Rius, A.

    1984-01-01

    A complete sample of 26 extended radio galaxies was observed at 2.29 GHz with the Mark III VLBI system. The fringe spacing was about 3 milli-arcsec, and the detection limit was about 2 millijanskys. Half of the galaxies were found to possess milli-arcsec radio cores. In all but three sources, the nuclear flux density was less than 0.04 of the total flux density. Galaxies with high optical luminosity (less than -21.2) were more likely than less luminous galaxies to contain a detectable milliparcsec radio core (69 percent vs. 20 percent). For objects with arcsec cores, 80 percent were found to have a milli-arcsec core, even though the milli-arcsec object did not always contribute the greater part of the arcsec flux density.

  3. Core-level positive-ion and negative-ion fragmentation of gaseous and condensed HCCl3 using synchrotron radiation

    Science.gov (United States)

    Lu, K. T.; Chen, J. M.; Lee, J. M.; Haw, S. C.; Liang, Y. C.; Deng, M. J.

    2011-07-01

    We investigated the dissociation dynamics of positive-ion and negative-ion fragments of gaseous and condensed HCCl3 following photoexcitation of Cl 2p electrons to various resonances. Based on ab initio calculations at levels HF/cc-pVTZ and QCISD/6-311G*, the first doublet structures in Cl L-edge x-ray absorption spectrum of HCCl3 are assigned to transitions from the Cl (2P3/2,1/2) initial states to the 10a1* orbitals. The Cl 2p → 10a1* excitation of HCCl3 induces a significant enhancement of the Cl+ desorption yield in the condensed phase and a small increase in the HCCl+ yield in the gaseous phase. Based on the resonant photoemission of condensed HCCl3, excitations of Cl 2p electrons to valence orbitals decay predominantly via spectator Auger transitions. The kinetic energy distributions of Cl+ ion via the Cl 2p → 10a1* excitation are shifted to higher energy ˜0.2 eV and ˜0.1 eV relative to those via the Cl 2p → 10e* excitation and Cl 2p → shape resonance excitation, respectively. The enhancement of the yields of ionic fragments at specific core-excited resonance states is assisted by a strongly repulsive surface that is directly related to the spectator electrons localized in the antibonding orbitals. The Cl- anion is significantly reinforced in the vicinity of Cl 2p ionization threshold of gaseous HCCl3, mediated by photoelectron recapture through post-collision interaction.

  4. MicroRNA-409-3p inhibits osteosarcoma cell migration and invasion by targeting catenin-δ1.

    Science.gov (United States)

    Wu, Shifeng; Du, Xinjie; Wu, Manwu; Du, Hechun; Shi, Xiaoliang; Zhang, Tao

    2016-06-10

    Osteosarcoma is the most common primary bone cancer which is associated with early metastatic potential and poor prognosis. However, the molecular mechanisms underlying osteosarcoma progression are not well characterized. Here, we investigated the role of miR-409-3p in osteosarcoma metastasis. Osteosarcoma tissue showed decreased expression of miR-409-3p compared to adjacent non-tumorous tissue. The expression level of miR-409-3p was negatively correlated with osteosarcoma metastasis. Overexpression of miR-409-3p in osteosarcoma cells (U2OS) inhibited cell migration and invasion. Bioinformatics analysis showed that catenin-δ1 (CTNND1, p120-catenin) is a direct target of miR-409-3p. Overexpression of miR-409-3p repressed the expression of catenin-δ1 in U2OS cells at both mRNA and protein levels. Meanwhile, miR-409-3p repressed the activity of luciferase reporter containing the 3'-untranslated region (3'UTR) of CTNND1 gene. Furthermore, expression of catenin-δ1 rescued the inhibitory effect of miR-409-3p on cell migration and invasion. Altogether, these results indicated that miR-409-3p targets catenin-δ1 to repress osteosarcoma metastasis.

  5. miR-342-3p affects hepatocellular carcinoma cell proliferation via regulating NF-κB pathway

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, Liang; Zhang, Yubao, E-mail: zhyb880077@sina.com

    2015-02-13

    Recent research indicates that non-coding microRNAs (miRNAs) help regulate basic cellular processes in many types of cancer cells. We hypothesized that overexpression of miR-342-3p might affect proliferation of hepatocellular carcinoma (HCC) cells. After confirming overexpression of miR-342-3p with qRT-PCR, MTT assay showed that HCC cell proliferation was significantly inhibited by miR-342-3p, and that it significantly decreased BrdU-positive cell proliferation by nearly sixfold. Searching for targets using three algorithms we found that miR-342-3p is related to the NF-κB pathway and luciferase assay found that IKK-γ, TAB2 and TAB3 are miR-342-3p target genes. Results of western blot on extracted nuclear proteins of HepG2 and HCT-116 cells showed that miR-342-3p reduced and miR-342-3p-in increased p65 nuclear levels and qRT-PCR found that NF-κB pathway downstream genes were downregulated by miR-342-3p and upregulated by miR-342-3p-in, confirming that miR-342 targets NF-κB pathway. Overexpression of Ikk-γ, TAB2 and TAB3 partially rescued HCC cells proliferation inhibited by miR-342-3p. Using the GSE54751 database we evaluated expression from 10 HCC samples, which strongly suggested downregulation of miR-342-3p and we also found inverse expression between miR-342-3p and its targets IKK-γ, TAB2 and TAB3 from 71 HCC samples. Our results show that miR-342-3p has a significant role in HCC cell proliferation and is suitable for investigation of therapeutic targets. - Highlights: • MiR-342-3p suppresses hepatocellular carcinoma cell proliferation. • MiR-342-3p targets IKK-γ, TAB2 and TAB3 genes. • MiR-342-3p downregulates NF-kB signaling pathway. • MiR-342-3p is downregulated in clinical hepatocellular carcinoma samples. • The expression of miR-342-3p and its target gene is inversely related.

  6. Repumping of ultracold strontium atoms using the ^3P2 - ^3D2 transition

    Science.gov (United States)

    Mickelson, P. G.; Martinez de Escobar, Y. N.; Traverso, A. J.; Killian, T. C.

    2008-05-01

    We discuss recent experiments involving ultracold strontium. Using a commercially-available 3 micron laser, we repump atoms out of the ^3P2 level via the ^3D2 state and gain almost a factor of 10 in the number of atoms in our system. This increase in the signal-to-noise ratio enables improved spectroscopy of strontium in our optical trap.

  7. Preservice Secondary Teachers Perceptions of College-Level Mathematics Content Connections with the Common Core State Standards for Mathematics

    Science.gov (United States)

    Olson, Travis A.

    2016-01-01

    Preservice Secondary Mathematics Teachers (PSMTs) were surveyed to identify if they could connect early-secondary mathematics content (Grades 7-9) in the Common Core State Standards for Mathematics (CCSSM) with mathematics content studied in content courses for certification in secondary teacher preparation programs. Respondents were asked to…

  8. Genistein up-regulates tumor suppressor microRNA-574-3p in prostate cancer.

    Directory of Open Access Journals (Sweden)

    Takeshi Chiyomaru

    Full Text Available Genistein has been shown to inhibit cancers both in vitro and in vivo, by altering the expression of several microRNAs (miRNAs. In this study, we focused on tumor suppressor miRNAs regulated by genistein and investigated their function in prostate cancer (PCa and target pathways. Using miRNA microarray analysis and real-time RT-PCR we observed that miR-574-3p was significantly up-regulated in PCa cells treated with genistein compared with vehicle control. The expression of miR-574-3p was significantly lower in PCa cell lines and clinical PCa tissues compared with normal prostate cells (RWPE-1 and adjacent normal tissues. Low expression level of miR-574-3p was correlated with advanced tumor stage and higher Gleason score in PCa specimens. Re-expression of miR-574-3p in PCa cells significantly inhibited cell proliferation, migration and invasion in vitro and in vivo. miR-574-3p restoration induced apoptosis through reducing Bcl-xL and activating caspase-9 and caspase-3. Using GeneCodis software analysis, several pathways affected by miR-574-3p were identified, such as 'Pathways in cancer', 'Jak-STAT signaling pathway', and 'Wnt signaling pathway'. Luciferase reporter assays demonstrated that miR-574-3p directly binds to the 3' UTR of several target genes (such as RAC1, EGFR and EP300 that are components of 'Pathways in cancer'. Quantitative real-time PCR and Western analysis showed that the mRNA and protein expression levels of the three target genes in PCa cells were markedly down-regulated with miR-574-3p. Loss-of-function studies demonstrated that the three target genes significantly affect cell proliferation, migration and invasion in PCa cell lines. Our results show that genistein up-regulates tumor suppressor miR-574-3p expression targeting several cell signaling pathways. These findings enhance understanding of how genistein regulates with miRNA in PCa.

  9. H2S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expression

    Science.gov (United States)

    Li, Xing-Hui; Xue, Wen-Long; Wang, Ming-Jie; Zhou, Yu; Zhang, Cai-Cai; Sun, Chen; Zhu, Lei; Liang, Kun; Chen, Ying; Tao, Bei-Bei; Tan, Bo; Yu, Bo; Zhu, Yi-Chun

    2017-01-01

    The aims of the present study are to determine whether hydrogen sulfide (H2S) is involved in the expression of endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) production, and to identify the role of microRNA-455-3p (miR-455-3p) during those processes. In cultured human umbilical vein endothelial cells (HUVECs), the expression of miR-455-3p, eNOS protein and the NO production was detected after administration with 50 μM NaHS. The results indicated that H2S could augment the expression of miR-455-3p and eNOS protein, leading to the increase of NO level. We also found that overexpression of miR-455-3p in HUVECs increased the protein levels of eNOS whereas inhibition of miR-455-3p decreased it. Moreover, H2S and miR-455-3p could no longer increase the protein level of eNOS in the presence of proteasome inhibitor, MG-132. In vivo, miR-455-3p and eNOS expression were considerably increased in C57BL/6 mouse aorta, muscle and heart after administration with 50 μmol/kg/day NaHS for 7 days. We also identified that H2S levels and miR-455-3p expression increased in human atherosclerosis plaque while H2S levels decreased in plasma of atherosclerosis patients. Our data suggest that the stability of eNOS protein and the NO production could be regulated by H2S through miR-455-3p. PMID:28322298

  10. Control of Saturation level in the magnetic core of a welding transformer by Hysteresis Controller (HC and Proportional Integral (PI Controller

    Directory of Open Access Journals (Sweden)

    Rama Subbanna.S

    2016-12-01

    Full Text Available The objective of this paper is to analyse the performances of two controllers such as Hysteresis control (HC and proportional integral (PI control to control saturation level in the magnetic core of a welding transformer in a middle-frequency direct current (MFDC resistance spot welding system(RSWS. It consists of an input converter, welding transformer, and a full-wave rectifier mounted at the transformer secondary. The unequal ohmic resistances of the two transformer’s secondary circuits and the different characteristics of the diodes of output rectifier certainly lead to the magnetic core saturation which, consequently, causes the unwanted spikes in the transformer’s primary current and over-current protection switch-off. The goal is to analyse the performance of both controllers in terms of transients, total harmonic distortion(THD and variations in primary current and flux in the magnetic core of a welding transformer of highly nonlinear system of RSWS. The simulation study has been done in Matlab/Simulink environment and presented performance analysis. The responses shows that from the aforementioned aspects, proportional integral Controller is the better choice for controlling the saturation level in magnetic core of a welding transformer which is widely used in automobile industry welding system.

  11. A new form of Ca3P2 with a ring of Dirac nodes

    Directory of Open Access Journals (Sweden)

    Lilia S. Xie

    2015-08-01

    Full Text Available We report the synthesis and crystal structure of a new high-temperature form of Ca3P2. The crystal structure was determined through Rietveld refinements of synchrotron powder x-ray diffraction data. This form of Ca3P2 has a crystal structure of the hexagonal Mn5Si3 type, with a Ca ion deficiency compared to the ideal 5:3 stoichiometry. This yields a stable, charge-balanced compound of Ca2+ and P3−. We also report the observation of a secondary hydride phase, Ca5P3H, which again is a charge-balanced compound. The calculated band structure of Ca3P2 indicates that it is a three-dimensional Dirac semimetal with a highly unusual ring of Dirac nodes at the Fermi level. The Dirac states are protected against gap opening by a mirror plane in a manner analogous to what is seen for graphene.

  12. Oscillator strength for 3s{sup 2}3p{sup 5}-3s3p{sup 6} in Cl-like ions

    Energy Technology Data Exchange (ETDEWEB)

    Berrington, K.A.; Waldock, J.A. [School of Science and Mathematics, Sheffield Hallam University, Sheffield (United Kingdom); Pelan, J.C [Gatsby Computational Neuroscience Unit, University College, London (United Kingdom)

    2001-07-14

    Recent experiments have revealed discrepancies between calculated and experimental lifetimes in the lowest excited level of some chlorine-like ions. New ab initio oscillator strengths using the Breit-Pauli R-matrix method are provided for the 3s{sup 2}3p{sup 5} {sup 2}P{sup o}{sub 3/2,1/2}-3s3p{sup 6} {sup 2}S{sup e}{sub 1/2} transition in Cl-like Ar II through to Cu XIII. Our calculated lifetimes for these twelve ions are respectively 5.507, 2.077, 1.177, 0.784, 0.569, 0.438, 0.350, 0.287, 0.241, 0.206, 0.178 and 0.156 ns, and are more consistent with experiments than other calculations. (author). Letter-to-the-editor.

  13. Reconstructing Winter North Pacific Sea-Level Pressure Anomalies Over the Past Three Centuries Using a New Calibration Method with the Eclipse and Mt. Logan Ice Cores

    Science.gov (United States)

    Kelsey, E. P.; Wake, C. P.; Osterberg, E. C.

    2012-12-01

    A deeper understanding of the behavior of North Pacific extratropical cyclones and anticyclones prior to the instrumental era is needed to advance our understanding of North Pacific climate variability. To help achieve this objective, we develop and use a new nonlinear ice core calibration procedure with the Eclipse (3017 m a.s.l.) and Mt. Logan (5400 m a.s.l.) ice core records from Yukon, Canada to isolate the ranges of ice core values that are consistently associated with North Pacific wintertime sea-level pressure (SLP) anomalies. Over the calibration period (1872-2001), each ice core record is ranked and divided into 10 groups of 13 years. Then for each group, the frequency of positive and negative SLP anomalies at each grid point is contoured and the composite mean SLP anomaly values are shaded. These plots elucidate areas where statistically significant SLP anomalies occur frequently in association with groups of ice core values. This new calibration procedure shows that the lowest and the two highest groups of Mt. Logan annual [Na+] are sensitive to SLP anomalies in the central and eastern Pacific and the second lowest [Na+] group is sensitive to western Pacific SLP anomalies. The highest and lowest Eclipse cold-season accumulation groups are most sensitive to SLP anomalies more distant in the western and central Pacific. This result is surprising in light of stable isotope studies suggesting a more distant moisture source for Mt. Logan. A reconstruction using these calibrated records indicates the Aleutian Low was predominantly weaker than average between 1699-1871. Our results highlight that having these geographically close ice core records is important to developing a deeper understanding of North Pacific climate variability.

  14. MicroRNA-27a-3p Inhibits Melanogenesis in Mouse Skin Melanocytes by Targeting Wnt3a.

    Science.gov (United States)

    Zhao, Yuanyuan; Wang, Pengchao; Meng, Jinzhu; Ji, Yuankai; Xu, Dongmei; Chen, Tianzhi; Fan, Ruiwen; Yu, Xiuju; Yao, Jianbo; Dong, Changsheng

    2015-05-14

    MicroRNAs (miRNAs) play an essential role in the regulation of almost all the biological processes, including melanogenesis. MiR-27a-3p is nearly six times higher in white alpaca skin compared to brown skin, which indicates that miR-27a-3p may be a candidate regulator for melanogenesis. Wnt3a plays an important role in promoting melanoblasts to differentiate into melanocytes and melanogenesis. To confirm the function of miR-27a-3p to melanogenesis in mammals, miR-27a-3p mimic, inhibitor and their negative control were transfected into mouse melanocytes. As a result, miR-27a-3p inhibits melanogenesis by repressing Wnt3a at post-transcriptional level. A significant decrease in Wnt3a luciferase activity was observed in 293T cells co-transfected with the matched luciferase reporter vector and pre-miR-27a. Furthermore, the presence of exogenous miR-27a-3p significantly decreased Wnt3a protein expression rather than mRNA and reduced β-catenin mRNA levels in melanocytes. The over-expression of miR-27a-3p significantly increased the melanin content of melanocytes. However, miR-27a-3p inhibitor performs an opposite effect on melanogenesis. Wnt3a is one target of miR-27a-3p. MiR-27a-3p could inhibit Wnt3a protein amount by post-transcriptional regulation and melanogenesis in mouse melanocytes. Previous studies reported that Wnt3a promoted melanogenensis in mouse melanocytes. Thus, miR-27-3p inhibits melanogenesis by repressing Wnt3a protein expression.

  15. Self-consistent Green’s-function technique for bulk and surface impurity calculations: Surface core-level shifts by complete screening

    DEFF Research Database (Denmark)

    Aldén, M.; Abrikosov, I. A.; Johansson, B.;

    1994-01-01

    We have implemented an efficient self-consistent Green's-function technique, based on the tight-binding linear-muffin-tin-orbitals method, for calculating the electronic structure and total energy of a substitutional impurity located either in the bulk or at the surface. The technique makes use......'s-function impurity technique in a comprehensive study of the surface core-level shifts (SCLS) of the 4d and 5d transition metals. In those cases, where observed data refer to single crystals, we obtain good agreement with experiment, whereas the calculations typically underestimate the measured shift obtained from...... a polycrystalline surface. Comparison is made with independent theoretical data for the surface core-level eigenvalue shift, and the much debated role of the so-called initial-and final-state contributions to the SCLS is discussed....

  16. Spin-Orbit Effects in Spin-Resolved L2,3 Core Level Photoemission of 3d Ferromagnetic Thin Films

    Energy Technology Data Exchange (ETDEWEB)

    Komesu, T; Waddill, G D; Yu, S W; Butterfield, M; Tobin, J G

    2007-10-02

    We present spin-resolved 2p core level photoemission for the 3d transition metal films of Fe and Co grown on Cu(100). We observe clear spin asymmetry in the main 2p core level photoemission peaks of Fe and Co films consistent with trends in the bulk magnetic moments. The spin polarization can be strongly enhanced, by variation of the experimental geometry, when the photoemission is undertaken with circularly polarized light, indicating that spin-orbit interaction can have a profound in spin polarized photoemission. Further spin polarized photoemission studies using variable circularly polarized light at high photon energies, high flux are indicated, underscoring the value of synchrotron measurements at facilities with increased beam stability.

  17. Electronic Charges and Electric Potential at LaAlO3/SrTiO3 Interfaces Studied by Core-Level Photoemission Spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Hwang, Harold

    2011-08-19

    We studied LaAlO{sub 3}/SrTiO{sub 3} interfaces for varying LaAlO{sub 3} thickness by core-level photoemission spectroscopy. In Ti 2p spectra for conducting 'n-type' interfaces, Ti{sup 3+} signals appeared, which were absent for insulating 'p-type' interfaces. The Ti{sup 3+} signals increased with LaAlO{sub 3} thickness, but started well below the critical thickness of 4 unit cells for metallic transport. Core-level shifts with LaAlO{sub 3} thickness were much smaller than predicted by the polar catastrophe model. We attribute these observations to surface defects/adsorbates providing charges to the interface even below the critical thickness.

  18. Core-level X-ray photoemission spectral shift through the successive phase transitions in layered TlInS{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Ishizu, Takahiko [Department of Mathematical Sciences, Graduate School of Engineering, Osaka Prefecture University, Sakai 599-8531 (Japan); Mimura, Kojiro [Department of Mathematical Sciences, Graduate School of Engineering, Osaka Prefecture University, Sakai 599-8531 (Japan)], E-mail: mimura@ms.osakafu-u.ac.jp; Shim, Yong Gu [Department of Physics and Electronics, Graduate School of Engineering, Osaka Prefecture University, Sakai 599-8531 (Japan); Wakita, Kazuki [Department of Electrical, Electronics and Computer Engineering, Chiba Institute of Technology, Narashino 275-0016 (Japan); Mamedov, Nazim [Institute of Physics, Azerbaijan National Academy of Science, Baku, AZ-1143 (Azerbaijan); Taguchi, Yukihiro; Ichikawa, Kouichi [Department of Mathematical Sciences, Graduate School of Engineering, Osaka Prefecture University, Sakai 599-8531 (Japan)

    2008-12-31

    Temperature-dependent change in core-level electronic structures of the layered semiconductor-ferroelectric TlInS{sub 2} with incommensurate phase has been investigated by means of X-ray photoemission spectroscopy. The temperature dependence of the relative peak position for each core level (Tl 4f, In 3d and S 2p) is found to differ very much in the regions bordering each other at the normal-incommensurate phase transition point of 218 K. The obtained data suggest that the charge distribution in TlInS{sub 2} dramatically changes upon passing from the normal phase (T > 218 K) to the spatially modulated incommensurate phase (T < 218 K)

  19. Observation of core-level binding energy shifts between (100) surface and bulk atoms of epitaxial CuInSe{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Nelson, A.J. [Colorado School of Mines, Golden, CO (United States); Berry, G.; Rockett, A. [Univ. of Illinois, Urbana-Champaign, IL (United States)] [and others

    1997-04-01

    Core-level and valence band photoemission from semiconductors has been shown to exhibit binding energy differences between surface atoms and bulk atoms, thus allowing one to unambiguously distinguish between the two atomic positions. Quite clearly, surface atoms experience a potential different from the bulk due to the lower coordination number - a characteristic feature of any surface is the incomplete atomic coordination. Theoretical accounts of this phenomena are well documented in the literature for III-V and II-VI semiconductors. However, surface state energies corresponding to the equilibrium geometry of (100) and (111) surfaces of Cu-based ternary chalcopyrite semiconductors have not been calculated or experimental determined. These compounds are generating great interest for optoelectronic and photovoltaic applications, and are an isoelectronic analog of the II-VI binary compound semiconductors. Surface core-level binding energy shifts depend on the surface cohesive energies, and surface cohesive energies are related to surface structure. For ternary compound semiconductor surfaces, such as CuInSe{sub 2}, one has the possibility of variations in surface stoichiometry. Applying standard thermodynamical calculations which consider the number of individual surface atoms and their respective chemical potentials should allow one to qualitatively determine the magnitude of surface core-level shifts and, consequently, surface state energies.

  20. Hybridization and crystal-field effects in Kondo insulators studied by means of core-level spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Strigari, Fabio

    2015-04-13

    and even for symmetries lower than tetragonal. In addition to that, HAXPES measurements on the CeM{sub 2}Al{sub 10} series are presented. A common technique for studying hybridization effects in rare earths, and their electronic structure in general, is photoelectron spectroscopy in the soft X-ray range (hv ≤ 1.5 keV). However, in this energy region surface effects are known to matter so that the picture about the hybridization interaction might be distorted with respect to the bulk. The use of hard X-rays (hν=5-10 keV) guarantees a sufficiently large probing depth for obtaining information about the actual bulk electronic structure. In a detailed quantitative analysis of HAXPES 3d core level spectra - using a combination of full multiplet calculations and a configuration interaction model (fm-CI model) - the hybridization strength can be quantified. The XAS results show that the CEF ground states of CeRu{sub 2}Al{sub 10} and CeOs{sub 2}Al are very similar, while it is clearly different for the non-ordering system CeFe{sub 2}Al{sub 10}. The CEF description nicely explains the magnetic anisotropy observed in susceptibility data and to a large extent the small ordered moments along the c axis. We provide a reliable quantitative description of the CEF ground state of the CeM{sub 2}Al{sub 10} compounds. Furthermore, the analysis of the HAXPES data in the fm-CI model allows to quantify the intermediate 4f valence and establishes that the exchange interaction increases within the series from Ru to Os to Fe. A substantial amount of Kondo screening is shown to be present even in the magnetically ordered Ru and Os compounds. The polarized XAS study on CeNiSn demonstrates that the monoclinic CEF is well described in a trigonal approximation, and the determined 4f ground-state wave function is consistent with results from inelastic neutron scattering for Cu-doped CeNiSn. Moreover, the systematic investigation of the CeRh{sub 1-x}Ir{sub x}In{sub 5} substitution series by means

  1. Correlation between serum microRNA-124-3p and silent information regulator 1 levels in type 2 diabetic patients with different stages of diabetic kidney disease%2型糖尿病不同尿白蛋白排泄率患者血清微小RNA-124-3p与沉默信息调节因子1水平及其相关性

    Institute of Scientific and Technical Information of China (English)

    邵滢; 任慧雯; 吴灿; 吕川; 安丽; 王秋月

    2016-01-01

    -3p可能成为早期诊断糖尿病肾病新的生物学标记物。血清Mir-124-3p可能通过促进慢性炎症反应、肾脏纤维化等途径参与糖尿病肾病的发生和发展。%Objective To investigate changes of serum microRNA(Mir)-124-3p and silent information regulator 1(Sirt1) levels in type 2 diabetic patients with different stages of diabetic nephropathy. Methods Total of 508 cases with type 2 diabetes treated from September 2013 to September 2015 were divided into three groups according to the urinary albumin excretion rate: normoalbuminuric(D1, n=193), microalbuminuric(D2, n=175), macroalbuminuric group(D3, n=140). Serum levels of Mir-124-3p were validated by real-time polymerase chain reaction. Serum Sirt1, hypoxia-inducible factor-1α(HIF-1α), tumor necrosis factor-α(TNF-α) and fibronectin(FN) were determined by enzyme-linked immunosorbent assay (ELISA). One-way analysis of variance, Pearson correlation and multiple stepwise regression analysis were used for statistical analysis. Results The level of serum Mir-124-3p in type 2 diabetic patients (2.85 (2.78-2.92)ng/mg) was significantly higher than that in control group (0.91(0.87-0.95)ng/mg), and with the increase of urinary albumin excretion rate, the levels of serum Mir-124-3p in group D1, D2 and D3 increased gradually(F=1348.7, P<0.001). Compared with control, serum Sirt1 level decreased significantly in type 2 diabetic patients and decreased gradually in patients of D1, D2 and D3 groups(F=44.5, P<0.001). According to Pearson correlation analysis, age, diabetes duration, fasting plasma glucose, fasting insulin, homeostasis model assessment for insulin resistance(HOMA-IR), glycated hemoglobin A1c(HbA1c), low-density lipoprotein-cholesterol(LDL-C), total cholesterol(TC), triglyceride(TG), serum creatinine(Scr), blood urea nitrogen(BUN), uric acid(UA), HIF-1α, Mir-124-3p, FN and TNF-αwere positively correlated with Ln(ACR) (r=0.105-0.813, P<0.05); high-density lipoprotein

  2. Global ice volume during MIS 3 inferred from a sea-level analysis of sedimentary core records in the Yellow River Delta

    Science.gov (United States)

    Pico, Tamara; Mitrovica, Jerry X.; Ferrier, Ken L.; Braun, Jean

    2016-11-01

    Estimates of global ice volume during the glacial phase of the most recent ice age cycle are characterized by significant uncertainty, reflecting the relative paucity of geological constraints on sea level relevant to this time interval. For example, during the middle stages of Marine Isotope Stage 3, published estimates of peak global mean sea level (GMSL) relative to the present range from -25 m to -87 m. The large uncertainty in GMSL at MIS 3 has significant implications for estimates of the rate of ice growth in the period leading to the Last Glacial Maximum (∼26 ka). We refine estimates of global ice volume during MIS 3 by employing sediment cores in the Bohai and Yellow Sea that record a migration of the paleoshoreline at ∼50-37 ka through a transition from marine to brackish conditions. In particular, we correct relative sea level at these sites for contamination due to glacial isostatic adjustment using a sea-level calculation that includes a gravitationally self-consistent treatment of sediment redistribution and compaction, and estimate a peak global mean sea level of -38 ± 7 m during the interval 50-37 ka. With suitable sedimentary core records, the approach described herein can be extended to refine existing constraints on global ice volume across the entire glacial period.

  3. MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1

    Energy Technology Data Exchange (ETDEWEB)

    Gu, Haihua; Yang, Tao; Fu, Shaozi; Chen, Xiaofan; Guo, Lei; Ni, Yiming, E-mail: ni_yiming@hotmail.com

    2014-01-31

    Highlights: • We examined the level of miR-490-3p in A549 lung cancer cells compared with normal bronchial epithelial cell line. • We are the first to show the function of miR-490-3p in A549 lung cancer cells. • We demonstrate CCND1 may be one of the targets of miR-490-3p. - Abstract: MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate the translation of messenger RNAs by binding their 3′-untranslated region (3′UTR). In this study, we found that miR-490-3p is significantly down-regulated in A549 lung cancer cells compared with the normal bronchial epithelial cell line. To better characterize the role of miR-490-3p in A549 cells, we performed a gain-of-function analysis by transfecting the A549 cells with chemically synthesized miR-490-3P mimics. Overexpression of miR-490-3P evidently inhibits cell proliferation via G1-phase arrest. We also found that forced expression of miR-490-3P decreased both mRNA and protein levels of CCND1, which plays a key role in G1/S phase transition. In addition, the dual-luciferase reporter assays indicated that miR-490-3P directly targets CCND1 through binding its 3′UTR. These findings indicated miR-490-3P could be a potential suppressor of cellular proliferation.

  4. Gluon fragmentation into {sup 3} P{sub J} quarkonium

    Energy Technology Data Exchange (ETDEWEB)

    Ma, J.P.

    1995-10-01

    The functions of the gluon fragmentation into {sup 3}P{sub j} quarkonium are calculated to order {alpha}{sup 2}{sub s}. With the recent progress in analysing quarkonium systems in QCD it is possible show how the so called divergence in the limit of the zero-binding energy, which is related to P-wave quarkonia, is treated correctly in the case of fragmentation functions. The obtained fragmentation functions satisfy explicitly at the order of {alpha} {sup 2}{sub s} the Altarelli-Parisi equation and when z {yields} 0 they behave as z{sup -1} as expected. 19 refs., 7 figs.

  5. miR-409-3p sensitizes colon cancer cells to oxaliplatin by inhibiting Beclin-1-mediated autophagy.

    Science.gov (United States)

    Tan, Shifan; Shi, Huijuan; Ba, Mingchen; Lin, Shengqv; Tang, Hongsheng; Zeng, Xiaoqi; Zhang, Xiangliang

    2016-04-01

    The chemoresistance of colon cancer cells limits the efficacy of chemotherapy. miR-409-3p has been shown to be downregulated in various types of cancer. In the present study, we examined the role of miR-409-3p in colon cancer as well as the effects of miR‑409-3p on the sensitivity of colon cancer cells to oxaliplatin. The expression of miR-409 was significantly downregulated in the human colon cancer cell lines compared with the normal colon epithelial cells. Importantly, the miR-409-3p expression levels were lower in human colon cancer patient samples than in normal colon tissues. Moreover, we observed a negative correlation between the miR‑409-3p levels and resistance to oxaliplatin: the oxaliplatin-resistant colon cancer cells exhibited significantly downregulated miR‑409-3p levels, but higher autophagic activity than the oxaliplatin-sensitive cells. Using bioinformatics analysis, we predicted that miR‑409-3p miRNA binds to the key autophagy gene encoding Beclin-1. Our findings indicated that the overexpression of miR‑409-3p inhibited Beclin-1 expression and autophagic activity by binding to the 3'-untranslated region of Beclin-1 mRNA. In addition, the overexpression of miR‑409-3p enhanced the chemosensitivity of the oxaliplatin-sensitive and oxaliplatin-resistant colon cancer cells. The restoration of Beclin-1 abrogated these effects of miR‑409-3p. In a xenograft model using nude mice, we examined the effects of miR‑409-3p on tumor growth during chemotherapy. miR‑409-3p overexpression sensitized the tumor to chemotherapy, while inhibiting chemotherapy-induced autophagy in a manner dependent on Beclin-1. The findings of our study suggest that miR-409-3p is capable of enhancing the chemosensitivity of colon cancer cells by inhibiting Beclin-1-mediated autophagy.

  6. Suppressed ion chromatography methods for the routine determination of ultra low level anions and cations in ice cores.

    Science.gov (United States)

    Curran, M A; Palmer, A S

    2001-06-01

    The concentration of trace ionic species in snow and ice samples was determined using suppressed ion chromatography (IC) with conductivity detection and ultra-clean sample preparation techniques. Trace anion species were determined in a single 24-min run by combining sample preconcentration with gradient elution using Na2B4O7 eluent. The detection limits (ranging from 0.001 to 0.006 microM) are the lowest reported in the literature. Cation species were analysed by direct injection of 0.25 ml and isocratic elution with a H2SO4 eluent. The clean preparation techniques showed no evidence of a difference (Student's t-test) between Milli-Q water samples analysed directly and processed Milli-Q ice samples. These robust, ultra-clean IC methods were routinely applied to the analysis of large number of samples to produce a high-resolution trace ion ice core record from Law Dome, East Antarctica.

  7. Products of the Benzene + O(3P) Reaction

    Energy Technology Data Exchange (ETDEWEB)

    Taatjes, Craig A.; Osborn, David L.; Selby, Talitha M.; Meloni, Giovanni; Trevitt, Adam J.; Epifanovsky, Evgeny; Krylov, Anna I.; Sirjean, Baptiste; Dames, Enoch; Wang, Hai

    2009-12-21

    The gas-phase reaction of benzene with O(3P) is of considerable interest for modeling of aromatic oxidation, and also because there exist fundamental questions concerning the prominence of intersystem crossing in the reaction. While its overall rate constant has been studied extensively, there are still significant uncertainties in the product distribution. The reaction proceeds mainly through the addition of the O atom to benzene, forming an initial triplet diradical adduct, which can either dissociate to form the phenoxy radical and H atom, or undergo intersystem crossing onto a singlet surface, followed by a multiplicity of internal isomerizations, leading to several possible reaction products. In this work, we examined the product branching ratios of the reaction between benzene and O(3P) over the temperature range of 300 to 1000 K and pressure range of 1 to 10 Torr. The reactions were initiated by pulsed-laser photolysis of NO2 in the presence of benzene and helium buffer in a slow-flow reactor, and reaction products were identified by using the multiplexed chemical kinetics photoionization mass spectrometer operating at the Advanced Light Source (ALS) of Lawrence Berkeley National Laboratory. Phenol and phenoxy radical were detected and quantified. Cyclopentadiene and cyclopentadienyl radical were directly identified for the first time. Finally, ab initio calculations and master equation/RRKM modeling were used to reproduce the experimental branching ratios, yielding pressure-dependent rate expressions for the reaction channels, including phenoxy + H, phenol, cyclopentadiene + CO, which are proposed for kinetic modeling of benzene oxidation.

  8. Thermoelectric properties of polycrystalline NiSi3P4

    Science.gov (United States)

    May, Andrew F.; McGuire, Michael A.; Wang, Hsin

    2013-03-01

    The Hall and Seebeck coefficients, electrical resistivity, and thermal conductivity of polycrystalline NiSi3P4 were characterized from 2 to 775 K. Undoped NiSi3P4 behaves like a narrow gap semiconductor, with activated electrical resistivity ρ below room temperature and a large Seebeck coefficient of ˜400 μV/K at 300 K. Attempts to substitute boron for silicon resulted in the production of extrinsic holes, yielding moderately doped semiconductor behavior with ρ increasing with increasing temperature above ˜150 K. Hall carrier densities are limited to approximately 5 × 1019 cm-3 at 200 K, which would suggest the solubility limit of boron is reached if boron is indeed incorporated into the lattice. These extrinsic samples have a Hall mobility of ˜12 cm2/V/s at 300 K, and a parabolic band equivalent effective mass of ˜3.5 times the free electron mass. At 700 K, the thermoelectric figure of merit zT reaches ˜0.1. Further improvements in thermoelectric performance would require reaching higher carrier densities, as well as a mechanism to further reduce the lattice thermal conductivity, which is ˜5 W/m/K at 700 K. Alloying in Ge results in a slight reduction of the thermal conductivity at low temperatures, with little influence observed at higher temperatures.

  9. Core-level binding-energy shifts due to end effects in polymers: A Hartree-Fock Green's-function study

    Science.gov (United States)

    Seel, M.; Ladik, J.

    1985-10-01

    Hartree-Fock Green's-function studies of end effects on the core-level structure of metallic and insulating quasi-one-dimensional model polymers reveal additional core peaks outside the bulk bands. In the metallic case, shifts to both lower (~-150 meV) and higher (~+50 meV) binding energies are observed, whereas in the insulating case, split-off peaks occur only at the lower-binding-energy side (~-150 meV). It is shown that a positive or negative net valence population alone does not determine the direction of the shift. The binding-energy changes are determined by a detailed balance between the energy loss due to a decrease in the electron-nuclear attraction and the energy gain due to a decrease in the electron-electron repulsion experienced by the core electrons of the end atoms. This can probably also explain why for some metal surfaces, shifts towards lower, and for others, shifts towards higher, binding energies are found. In the valence region of the investigated lithium chains, the ends do not produce localized end states.

  10. Energetics and Dynamics of the Reactions of O(3P) with Dimethyl Methylphosphonate and Sarin

    Science.gov (United States)

    Conforti, Patrick F.; Braunstein, Matthew; Dodd, James A.

    2009-10-01

    Electronic structure and molecular dynamics calculations were performed on the reaction systems O(3P) + sarin and O(3P) + dimethyl methylphosphonate (DMMP), a sarin simulant. Transition state geometries, energies, and heats of reaction for the major reaction pathways were determined at several levels of theory, including AM1, B3LYP/6-311+G(d,p), and CBS-QB3. The major reaction pathways for both systems are similar and include H-atom abstraction, H-atom elimination, and methyl elimination, in rough order from low to high energy. The H-atom abstraction channels have fairly low barriers (˜10 kcal mol-1) and are close to thermoneutral, while the other channels have relatively high energy barriers (>40 kcal mol-1) and a wide range of reaction enthalpies. We have also found a two-step pathway leading to methyl elimination through O-atom attack on the phosphorus atom for DMMP and sarin. For sarin, the two-step methyl elimination pathway is significantly lower in energy than the single-step pathway. We also present results of O(3P) + sarin and O(3P) + DMMP reaction cross sections over a broad range of collision energies (2-10 km s-1 collision velocities) obtained using the direct dynamics method with an AM1 semiempirical potential. These excitation functions are intended as an approximate guide to future hyperthermal measurements, which to our knowledge have not yet examined either of these systems. The reaction barriers, reaction enthalpies, transition state structures, and excitation functions are generally similar for DMMP and sarin, with some moderate differences for methyl elimination energetics, which indicates DMMP will likely be a good substitute for sarin in many O(3P) chemical investigations.

  11. 1S core-level spectroscopy of graphite: The effects of phonons on emission and absorption and validity of the final-state rule

    Energy Technology Data Exchange (ETDEWEB)

    Franck, C.P.; Schnatterly, S.E.; Zutavern, F.J.; Aton, T.; Cafolla, T.; Carson, R.D.

    1985-04-15

    We have used both electron-induced soft x-ray emission and fast inelastic electron scattering to observe 1S core-level emission and absorption in graphite near threshold. Linear phonon coupling with partial relaxation is found to quantitatively explain the absorption linewidth, the emission broadening, and the unusually large difference between emission and absorption threshold energies (Stokes shift). Both emission and absorption line shapes quantitatively obey the final-state rule, which asserts that the best single-particle potential describing these many electron processes is the final-state potential.

  12. Proteomics Core

    Data.gov (United States)

    Federal Laboratory Consortium — Proteomics Core is the central resource for mass spectrometry based proteomics within the NHLBI. The Core staff help collaborators design proteomics experiments in a...

  13. Proteomics Core

    Data.gov (United States)

    Federal Laboratory Consortium — Proteomics Core is the central resource for mass spectrometry based proteomics within the NHLBI. The Core staff help collaborators design proteomics experiments in...

  14. sp2/sp3 hybridization ratio in amorphous carbon from C 1s core-level shifts: X-ray photoelectron spectroscopy and first-principles calculation

    Science.gov (United States)

    Haerle, Rainer; Riedo, Elisa; Pasquarello, Alfredo; Baldereschi, Alfonso

    2002-01-01

    Using a combined experimental and theoretical approach, we address C 1s core-level shifts in amorphous carbon. Experimental results are obtained by x-ray photoelectron spectroscopy (XPS) and electron-energy-loss spectroscopy (EELS) on thin-film samples of different atomic density, obtained by a pulsed-laser deposition growth process. The XPS spectra are deconvoluted into two contributions, which are attributed to sp2- and sp3-hybridized atoms, respectively, separated by 0.9 eV, independent of atomic density. The sp3 hybridization content extracted from XPS is consistent with the atomic density derived from the plasmon energy in the EELS spectrum. In our theoretical study, we generate several periodic model structures of amorphous carbon of different densities applying two schemes of increasing accuracy in sequence. We first use a molecular-dynamics approach, based on an environmental-dependent tight-binding Hamiltonian to quench the systems from the liquid phase. The final model structures are then obtained by further atomic relaxation using a first-principles pseudopotential plane-wave approach within density-functional theory. Within the latter framework, we also calculate carbon 1s core-level shifts for our disordered model structures. We find that the shifts associated to threefold- and fourfold- coordinated carbon atoms give rise to two distinct peaks separated by about 1.0 eV, independent of density, in close agreement with experimental observations. This provides strong support for decomposing the XPS spectra into two peaks resulting from sp2- and sp3-hybridized atoms. Core-hole relaxations effects account for about 30% of the calculated shifts.

  15. Performance of the TPSS Functional on Predicting Core Level Binding Energies of Main Group Elements Containing Molecules: A Good Choice for Molecules Adsorbed on Metal Surfaces.

    Science.gov (United States)

    Pueyo Bellafont, Noèlia; Viñes, Francesc; Illas, Francesc

    2016-01-12

    Here we explored the performance of Hartree-Fock (HF), Perdew-Burke-Ernzerhof (PBE), and Tao-Perdew-Staroverov-Scuseria (TPSS) functionals in predicting core level 1s binding energies (BEs) and BE shifts (ΔBEs) for a large set of 68 molecules containing a wide variety of functional groups for main group elements B → F and considering up to 185 core levels. A statistical analysis comparing with X-ray photoelectron spectroscopy (XPS) experiments shows that BEs estimations are very accurate, TPSS exhibiting the best performance. Considering ΔBEs, the three methods yield very similar and excellent results, with mean absolute deviations of ∼0.25 eV. When considering relativistic effects, BEs deviations drop approaching experimental values. So, the largest mean percentage deviation is of 0.25% only. Linear trends among experimental and estimated values have been found, gaining offsets with respect to ideality. By adding relativistic effects to offsets, HF and TPSS methods underestimate experimental values by solely 0.11 and 0.05 eV, respectively, well within XPS chemical precision. TPSS is posed as an excellent choice for the characterization, by XPS, of molecules on metal solid substrates, given its suitability in describing metal substrates bonds and atomic and/or molecular orbitals.

  16. Structural origin of Si-2p core-level shifts from Si(100)-c[4x2] surface: A spectral x-ray photoelectron diffraction study

    Energy Technology Data Exchange (ETDEWEB)

    Chen, X.; Tonner, B.P. [Univ. of Wisconsin, Milwaukee, WI (United States); Denlinger, J. [Univ. of Wisconsin, Milwaukee, WI (United States)][Ernest Orlando Lawrence Berkeley National Lab., CA (United States)] [and others

    1997-04-01

    The authors have performed angle-resolved x-ray photoelectron diffraction (XPD) from a Si(100)-c(4x2) surface to study the structural origin of Si-2p core-level shifts. In the experiment, the highly resolved surface Si-2p core-level spectra were measured as a fine grid of hemisphere and photon energies, using the SpectroMicroscopy Facility {open_quotes}ultraESCA{close_quotes} instrument. By carefully decomposing the spectra into several surface peaks, the authors are able to obtain surface-atom resolved XPD patterns. Using a multiple scattering analysis, they derived a detailed atomic model for the Si(100)-c(4x2) surface. In this model, the asymmetric dimers were found tilted by 11.5 plus/minus 2.0 degrees with bond length of 2.32 plus/minus 0.05{angstrom}. By matching model XPD patterns to experiment, the authors can identify which atoms in the reconstructed surface are responsible for specific photoemission lines in the 2p spectrum.

  17. Core-level spectra and binding energies of transition metal nitrides by non-destructive x-ray photoelectron spectroscopy through capping layers

    Science.gov (United States)

    Greczynski, G.; Primetzhofer, D.; Lu, J.; Hultman, L.

    2017-02-01

    We present the first measurements of x-ray photoelectron spectroscopy (XPS) core level binding energies (BE:s) for the widely-applicable group IVb-VIb polycrystalline transition metal nitrides (TMN's) TiN, VN, CrN, ZrN, NbN, MoN, HfN, TaN, and WN as well as AlN and SiN, which are common components in the TMN-based alloy systems. Nitride thin film samples were grown at 400 °C by reactive dc magnetron sputtering from elemental targets in Ar/N2 atmosphere. For XPS measurements, layers are either (i) Ar+ ion-etched to remove surface oxides resulting from the air exposure during sample transfer from the growth chamber into the XPS system, or (ii) in situ capped with a few nm thick Cr or W overlayers in the deposition system prior to air-exposure and loading into the XPS instrument. Film elemental composition and phase content is thoroughly characterized with time-of-flight elastic recoil detection analysis (ToF-E ERDA), Rutherford backscattering spectrometry (RBS), and x-ray diffraction. High energy resolution core level XPS spectra acquired with monochromatic Al Kα radiation on the ISO-calibrated instrument reveal that even mild etching conditions result in the formation of a nitrogen-deficient surface layer that substantially affects the extracted binding energy values. These spectra-modifying effects of Ar+ ion bombardment increase with increasing the metal atom mass due to an increasing nitrogen-to-metal sputter yield ratio. The superior quality of the XPS spectra obtained in a non-destructive way from capped TMN films is evident from that numerous metal peaks, including Ti 2p, V 2p, Zr 3d, and Hf 4f, exhibit pronounced satellite features, in agreement with previously published spectra from layers grown and analyzed in situ. In addition, the N/metal concentration ratios are found to be 25-90% higher than those obtained from the corresponding ion-etched surfaces, and in most cases agree very well with the RBS and ToF-E ERDA values. The N 1 s BE:s extracted from

  18. miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Peng; Wu, Dingguo; You, Yu; Sun, Jing; Lu, Lele; Tan, Jiaxing; Bie, Ping, E-mail: bieping2010@163.com

    2015-08-15

    MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at post-transcriptional level. miRNA dysregulation plays a causal role in cancer progression. In this study, miR-208-3p was highly expressed and directly repressed ARID2 expression. As a result, ARID2 expression in hepatocellular carcinoma (HCC) was decreased. In vitro, miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. In vivo test results revealed that miR-208-3p down-regulation inhibited HCC tumorigenesis in Hep3B cells. Moreover, ARID2 was possibly a downstream element of transforming growth factor beta1 (TGFβ1)/miR-208-3p/ARID2 regulatory pathway. These findings suggested that miR-208-3p up-regulation is associated with HCC cell progression and may provide a new target for liver cancer treatment. - Highlights: • miR-208-3p was highly expressed and directly repressed the expression of ARID2 in HCC. • miR-208-3p contributed to HCC cell progression both in vitro and in vivo. • Over-expression of ARID2 inhibited the HCC cell proliferation and invasion. • Restoration of ARID2 partly reversed the the effect of miR-208-3p down-regulation on HCC cells. • Newly regulatory pathway: miR-208-3p mediated the repression of ARID2 by TGFβ1 in HCC cells.

  19. Coinage metal complexes supported by a "PN(3)P" scaffold.

    Science.gov (United States)

    Rao, Gyandshwar Kumar; Gorelsky, Serge I; Korobkov, Ilia; Richeson, Darrin

    2015-11-28

    A series of monovalent group 11 complexes, [2,6-{Ph2PNMe}2(NC5H3)]CuBr 1, [2,6-{Ph2PNMe}2(NC5H3)]CuOTf 2, [2,6-{Ph2PNMe}2(NC5H3)]AgOTf 3, and [2,6-{Ph2PNMe}2(NC5H3)](AuCl)24, supported by a neutral PN(3)P ligand have been synthesized and characterized by multinuclear NMR and single crystal X-ray diffraction studies. The variation of the coordination properties were analyzed and electronic structure calculations have been carried out to provide insight on the bonding details in these complexes. The Cu(I) complexes displayed an unusual coordination geometry with a tridentate pincer ligand and an overall four coordinate trigonal pyramidal geometry. In contrast the Ag(I) analogue displayed a bidentate κ(2)-P,P' ligation leaving the pyridyl-N atom uncoordinated and yielding a pyramidalized trigonal planar geometry around Ag. The bimetallic Au(I) complex completed the series and displayed a monodentate P-bonded ligand and a linear coordination geometry.

  20. The Involvement of miR-29b-3p in Arterial Calcification by Targeting Matrix Metalloproteinase-2

    Science.gov (United States)

    Jiang, Wenhong; Zhang, Zhanman; Yang, Han; Lin, Qiuning; Han, Chuangye

    2017-01-01

    Vascular calcification is a risk predictor and common pathological change in cardiovascular diseases that are associated with elastin degradation and phenotypic transformation of vascular smooth muscle cells via gelatinase matrix metalloproteinase-2 (MMP2). However, the mechanisms involved in this process remain unclear. In this study, we investigated the relationships between miR-29b-3p and MMP2, to confirm miR-29b-3p-mediated MMP2 expression at the posttranscriptional level in arterial calcification. In male Sprague Dawley rats, arterial calcification was induced by subcutaneous injection of a toxic dose of cholecalciferol. In vivo, the quantitative real-time polymerase chain reaction (qRT-PCR) showed that MMP2 expression was upregulated in calcified arterial tissues, and miR-29b-3p expression was downregulated. There was a negative correlation between MMP2 mRNA expression and miR-29b-3p levels (P = 0.0014, R2 = 0.481). Western blotting showed that MMP2 expression was significantly increased in rats treated with cholecalciferol. In vitro, overexpression of miR-29b-3p led to decreased MMP2 expression in rat vascular smooth muscle cells, while downregulation of miR-29b-3p expression led to increased MMP2 expression. Moreover, the luciferase reporter assay confirmed that MMP2 is the direct target of miR-29b-3p. Together, our results demonstrated that a role of miR-29b-3p in vascular calcification involves targeting MMP2. PMID:28164126

  1. The Involvement of miR-29b-3p in Arterial Calcification by Targeting Matrix Metalloproteinase-2

    Directory of Open Access Journals (Sweden)

    Wenhong Jiang

    2017-01-01

    Full Text Available Vascular calcification is a risk predictor and common pathological change in cardiovascular diseases that are associated with elastin degradation and phenotypic transformation of vascular smooth muscle cells via gelatinase matrix metalloproteinase-2 (MMP2. However, the mechanisms involved in this process remain unclear. In this study, we investigated the relationships between miR-29b-3p and MMP2, to confirm miR-29b-3p-mediated MMP2 expression at the posttranscriptional level in arterial calcification. In male Sprague Dawley rats, arterial calcification was induced by subcutaneous injection of a toxic dose of cholecalciferol. In vivo, the quantitative real-time polymerase chain reaction (qRT-PCR showed that MMP2 expression was upregulated in calcified arterial tissues, and miR-29b-3p expression was downregulated. There was a negative correlation between MMP2 mRNA expression and miR-29b-3p levels (P=0.0014, R2=0.481. Western blotting showed that MMP2 expression was significantly increased in rats treated with cholecalciferol. In vitro, overexpression of miR-29b-3p led to decreased MMP2 expression in rat vascular smooth muscle cells, while downregulation of miR-29b-3p expression led to increased MMP2 expression. Moreover, the luciferase reporter assay confirmed that MMP2 is the direct target of miR-29b-3p. Together, our results demonstrated that a role of miR-29b-3p in vascular calcification involves targeting MMP2.

  2. Rapid measurement of perchlorate in polar ice cores down to sub-ng L(-1) levels without pre-concentration.

    Science.gov (United States)

    Peterson, Kari; Cole-Dai, Jihong; Brandis, Derek; Cox, Thomas; Splett, Scott

    2015-10-01

    An ion chromatography-electrospray ionization-tandem mass spectrometry (IC-ESI-MS/MS) method has been developed for rapid and accurate measurement of perchlorate in polar snow and ice core samples in which perchlorate concentrations are expected to be as low as 0.1 ng L(-1). Separation of perchlorate from major inorganic species in snow is achieved with an ion chromatography system interfaced to an AB SCIEX triple quadrupole mass spectrometer operating in multiple reaction monitoring mode. Under optimized conditions, the limit of detection and lower limit of quantification without pre-concentration have been determined to be 0.1 and 0.3 ng L(-1), respectively, with a linear dynamic range of 0.3-10.0 ng L(-1) in routine measurement. These represent improvements over previously reported methods using similar analytical techniques. The improved method allows fast, accurate, and reproducible perchlorate quantification down to the sub-ng L(-1) level and will facilitate perchlorate measurement in the study of natural perchlorate production with polar ice cores in which perchlorate concentrations are anticipated to vary in the low and sub-ng L(-1) range. Initial measurements of perchlorate in ice core samples from central Greenland show that typical perchlorate concentrations in snow dated prior to the Industrial Revolution are about 0.8 ng L(-1), while perchlorate concentrations are significantly higher in recent (post-1980) snow, suggesting that anthropogenic sources are a significant contributor to perchlorate in the current environment.

  3. Ypq3p-dependent histidine uptake by the vacuolar membrane vesicles of Saccharomyces cerevisiae.

    Science.gov (United States)

    Manabe, Kunio; Kawano-Kawada, Miyuki; Ikeda, Koichi; Sekito, Takayuki; Kakinuma, Yoshimi

    2016-06-01

    The vacuolar membrane proteins Ypq1p, Ypq2p, and Ypq3p of Saccharomyces cerevisiae are known as the members of the PQ-loop protein family. We found that the ATP-dependent uptake activities of arginine and histidine by the vacuolar membrane vesicles were decreased by ypq2Δ and ypq3Δ mutations, respectively. YPQ1 and AVT1, which are involved in the vacuolar uptake of lysine/arginine and histidine, respectively, were deleted in addition to ypq2Δ and ypq3Δ. The vacuolar membrane vesicles isolated from the resulting quadruple deletion mutant ypq1Δypq2Δypq3Δavt1Δ completely lost the uptake activity of basic amino acids, and that of histidine, but not lysine and arginine, was evidently enhanced by overexpressing YPQ3 in the mutant. These results suggest that Ypq3p is specifically involved in the vacuolar uptake of histidine in S. cerevisiae. The cellular level of Ypq3p-HA(3) was enhanced by depletion of histidine from culture medium, suggesting that it is regulated by the substrate.

  4. miR-4782-3p Inhibited Non-Small Cell Lung Cancer growth via USP14

    Directory of Open Access Journals (Sweden)

    Ning Wu

    2014-02-01

    Full Text Available Background: Lung cancer is the leading cause of cancer-related mortality worldwide, with near 1.4 million deaths each year. NSCLC accounts for nearly 85% of all case of lung cancer. MiRNAs play important roles in regulation of gene expression at the post-transcriptional level. MiRNAs profiles may predict prognosis and disease recurrence in early-stage NSCLC. Our previous study proved that over-expression of ubiquitin specific peptidase 14 (USP14, a deubiquitinating enzyme, was associated with favorable prognosis in NSCLC patients and promoted tumor cells proliferation. Here, we tried to identify which miRNAs targeted USP14, and the roles of these miRNAs in NSCLC. Methods: MiR-4782-3p and its potential targeted genes were identified by bioinformatics algorithm. Dual luciferase reporter assay system was used to analyze the interaction between miR-4782-3p and targeted genes. Cell proliferation was assayed by MTT and BdU assay. MiRNAs and mRNA expression were assayed by qRT-PCR. USP14 protein level was assayed by Western blot. The role of miR-4782-3p in patients survival was revealed by Kaplan-Meier plot of overall survival. Results: Up-expression of miR-4782-3p in NSCLC cells decreased the USP14 expression. Down-expression of miR-4782-3p increased USP14 expression. In NSCLC specimen, Negative correlation between USP14 mRNA level and miR-4782-3p level was identified. Higher miR-4782-3p expression is associated with longer survival. USP14, ZEB2, XIAP overexpression reversed the inhibitory effect of miR-4782-3p. Conclusions: High expression of miR-4782-3p was associated with favorable prognosis in NSCLC patients. MiR-4782-3p inhibited cell proliferation in NSCLC by targeting USP14, ZEB2 and XIAP.

  5. The Evolution of Ga and As Core Levels in the Formation of Fe/GaAs(001): A High Resolution Soft X-ray Photoelectron Spectroscopic Study

    Energy Technology Data Exchange (ETDEWEB)

    Thompson, J W; Neal, J R; Shen, T H; Morton, S A; Tobin, J G; Waddill, G D; Matthew, J D; Greig, D; Hopkinson, M

    2006-12-08

    A high resolution soft x-ray photoelectron spectroscopic study of Ga and As 3d core levels has been conducted for Fe/GaAs (001) as a function of Fe thickness. This work has provided unambiguous evidence of substrate disrupting chemical reactions induced by the Fe overlayer--a quantitative analysis of the acquired spectra indicates significantly differing behavior of Ga and As during Fe growth, and our observations have been compared with existing theoretical models. Our results demonstrate that the outdiffusing Ga and As remain largely confined to the interface region, forming a thin intermixed layer. Whereas at low coverages Fe has little influence on the underlying GaAs substrate, the onset of substrate disruption when the Fe thickness reaches 3.5 {angstrom} results in major changes in the energy distribution curves (EDCs) of both As and Ga 3d cores. Our quantitative analysis suggests the presence of two new As environments of metallic character; one bound to the interfacial region and another which, as confirmed by in-situ oxidation experiments, surface segregates and persists over a wide range of overlayer thickness. Analysis of the corresponding Ga 3d EDCs found not two, but three new environments--also metallic in nature. Two of the three are interface-resident whereas the third undergoes outdiffusion at low Fe coverages. Based on the variations of the integrated intensities of each component, we present a schematic of the proposed chemical make-up of the Fe/GaAs (001) system.

  6. The evolution of Ga and As core levels in the formation of Fe/GaAs (001):A high resolution soft x-ray photoelectron spectroscopic study

    Energy Technology Data Exchange (ETDEWEB)

    Thompson, Jamie; Neal, James; Shen, Tiehan; Morton, Simon; Tobin, James; Waddill, George Dan; Matthew, Jim; Greig, Denis; Hopkinson, Mark

    2008-07-14

    A high resolution soft x-ray photoelectron spectroscopic study of Ga and As 3d core levels has been conducted for Fe/GaAs (001) as a function of Fe thickness. This work has provided unambiguous evidence of substrate disrupting chemical reactions induced by the Fe overlayer--a quantitative analysis of the acquired spectra indicates significantly differing behavior of Ga and As during Fe growth, and our observations have been compared with existing theoretical models. Our results demonstrate that the outdiffusing Ga and As remain largely confined to the interface region, forming a thin intermixed layer. Whereas at low coverages Fe has little influence on the underlying GaAs substrate, the onset of substrate disruption when the Fe thickness reaches 3.5 Angstrom results in major changes in the energy distribution curves (EDCs) of both As and Ga 3d cores. Our quantitative analysis suggests the presence of two additional As environments of metallic character: one bound to the interfacial region and another which, as confirmed by in situ oxidation experiments, surface segregates and persists over a wide range of overlayer thickness. Analysis of the corresponding Ga 3d EDCs found not two, but three additional environments--also metallic in nature. Two of the three are interface resident whereas the third undergoes outdiffusion at low Fe coverages. Based on the variations of the integrated intensities of each component, we present a schematic of the proposed chemical makeup of the Fe/GaAs (001) system.

  7. The importance of relative mutant level for evaluating impact on outcome of KIT, FLT3 and CBL mutations in core-binding factor acute myeloid leukemia.

    Science.gov (United States)

    Allen, C; Hills, R K; Lamb, K; Evans, C; Tinsley, S; Sellar, R; O'Brien, M; Yin, J L; Burnett, A K; Linch, D C; Gale, R E

    2013-09-01

    Several different mutations collaborate with the fusion proteins in core-binding factor acute myeloid leukemia (CBF-AML) to induce leukemogenesis, but their prognostic significance remains unclear. We screened 354 predominantly younger (mutations and FLT3 internal tandem duplications (FLT3(ITD)) and assessed the impact of relative mutant level on outcome. Overall, 28% had KIT, 6% FLT3(ITD), 10% FLT3(TKD), 27% RAS and 6% CBL mutations. Mutant levels for all genes/loci were highly variable. KIT mutations were associated with a higher cumulative incidence of relapse but in multivariate analysis this was only significant for cases with a higher mutant level of 25% or greater (95% confidence interval (CI)=1.01-1.52, P=0.04). Similarly, only FLT3(ITD-HIGH) was a significant adverse factor for overall survival (OS; CI=1.27-5.39, P=0.004). Conversely, FLT3(TKD-HIGH) and CBL(HIGH) were both favorable factors for OS (CI= 0.31-0.89, P=0.01 and CI=0.05-0.85, P=0.02, respectively). KIT mutations were frequently lost at relapse, which is relevant to minimal residual disease detection and the clinical use of KIT inhibitors. These results indicate that relative mutant level should be taken into account when evaluating the impact of mutations in CBF-AML.

  8. Ginkgolide B Inhibits Human Bladder Cancer Cell Migration and Invasion Through MicroRNA-223-3p

    Directory of Open Access Journals (Sweden)

    Yi Zhi

    2016-10-01

    Full Text Available Background/Aims: Ginkgolide B (GB is currently used as an anticancer drug for treatment of some malignant cancers. However, whether it may have therapeutic effects on bladder cancer remains unknown. Here, we studied the effects of GB on bladder cancer cells. Methods: Bladder cells were treated with different doses of GB, and the effects on ZEB1 and microRNA-223-3p (miR-223-3p were analyzed by RT-qPCR and/or Western blot. Prediction of a regulatory relationship between miR-93 and 3'-UTR of Beclin-1 mRNA was performed by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Results: We found that GB dose-dependently decreased ZEB1 protein, but not mRNA, in bladder cancer cells, resulting in suppression of cell invasion. Moreover, in bladder cancer cells, GB dose-dependently decreased the levels of miR-223-3p, which suppressed the protein translation of ZEB1 through binding to 3'-UTR of ZEB1 mRNA. Overexpression of miR-223-3p decreased ZEB1 protein, while depletion of miR-223-3p increased ZEB1 protein in bladder cancer cells. Conclusion: GB inhibits bladder cancer cell invasiveness through suppressing ZEB1 protein translation via upregulating miR-223-3p.

  9. miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110.

    Science.gov (United States)

    Bijnsdorp, Irene V; Hodzic, Jasmina; Lagerweij, Tonny; Westerman, Bart; Krijgsman, Oscar; Broeke, Jurjen; Verweij, Frederik; Nilsson, R Jonas A; Rozendaal, Lawrence; van Beusechem, Victor W; van Moorselaar, Jeroen A; Wurdinger, Thomas; Geldof, Albert A

    2016-03-29

    The centrosome plays a key role in cancer invasion and metastasis. However, it is unclear how abnormal centrosome numbers are regulated when prostate cancer (PCa) cells become metastatic. CP110 was previously described for its contribution of centrosome amplification (CA) and early development of aggressive cell behaviour. However its regulation in metastatic cells remains unclear. Here we identified miR-129-3p as a novel metastatic microRNA. CP110 was identified as its target protein. In PCa cells that have metastatic capacity, CP110 expression was repressed by miR-129-3p. High miR-129-3p expression levels increased cell invasion, while increasing CP110 levels decreased cell invasion. Overexpression of CP110 in metastatic PCa cells resulted in a decrease in the number of metastasis. In tissues of PCa patients, low CP110 and high miR-129-3p expression levels correlated with metastasis, but not with the expression of genes related to EMT. Furthermore, overexpression of CP110 in metastatic PCa cells resulted in excessive-CA (E-CA), and a change in F-actin distribution which is in agreement with their reduced metastatic capacity. Our data demonstrate that miR-129-3p functions as a CA gatekeeper in metastatic PCa cells by maintaining pro-metastatic centrosome amplification (CA) and preventing anti-metastatic E-CA.

  10. 共沉默miR-221-3p/222-3p表达抑制骨髓间充质干细胞增殖及促成软骨分化%Down-regulation of miR-221-3p/222-3p inhibits cell proliferation and promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells

    Institute of Scientific and Technical Information of China (English)

    闫继红; 周德山; 杨姝; 孙海梅; 曹丹丹; 张秀英; 季凤清; 郭多; 吴波; 孙婷怡

    2015-01-01

    BACKGROUND:The use of mesenchymal stem cels in the field of tissue engineering for osteoarticular injury repair is a very promising tool since these cels are readily expandable and able to differentiate into chondrocytes. Abundant evidence suggests that microRNAs play critical roles in chondrogenic differentiation of mesenchymal stem cels. OBJECTIVE:To observe the chondrogenic effect of human bone marrow mesenchymal stem cels transfected with lentiviral vectors bearing miR-221-3p/222-3p inhibition, thereby provding new strategies for cartilage injury. METHODS: miRNA microarray technology was applied to detect microRNAs expression profiles at three different stages of chondrogenic differentiation induction after transforming growth factor-β3 treatment and verified by real-time fluorescence quantitative PCR (RT-qPCR). Human bone marrow mesenchymal stem cels were infected with lentivirus bearing miR-221-3p/222-3p inhibition. After co-suppressing the expression of miR-221/222-3p, cel counting kit-8 was used to determine the cel proliferation, the differentiation of bone marrow mesenchymal stem cels towards chondrocytes was verified by type II colagen protein expression through immunohistochemistry and glycosaminoglycan accumulation was also elevated by sarranine O staining. RT-PCR was used to detect type II colagen and aggrecan mRNA expression at 21 days of chondrogenic induction. RESULTS AND CONCLUSION: The expression of miR-221-3p/222-3p was inhibited after Lv-miR221-3p/222-3p inhibition co-transfected into bone marrow mesenchymal stem cels. microRNA microarray and RT-qPCR results showed that the expression of miR-221-3p/222-3p was declined significantly at the anaphase of chondrogenic differentiation. The expression levels of chondrogenic markers, Aggrecan and type II colagen were significantly increased in the miR-221-3p/222-3p inhibition group and cel proliferation was also inhibited significantly compared with non-transduced cels or transduced with the empty

  11. A suggested emergency medicine boot camp curriculum for medical students based on the mapping of Core Entrustable Professional Activities to Emergency Medicine Level 1 milestones

    Directory of Open Access Journals (Sweden)

    Lamba S

    2016-03-01

    Full Text Available Sangeeta Lamba, Bryan Wilson, Brenda Natal, Roxanne Nagurka, Michael Anana, Harsh Sule Department of Emergency Medicine, Rutgers New Jersey Medical School, Newark, NJ, USA Background: An increasing number of students rank Emergency Medicine (EM as a top specialty choice, requiring medical schools to provide adequate exposure to EM. The Core Entrustable Professional Activities (EPAs for Entering Residency by the Association of American Medical Colleges combined with the Milestone Project for EM residency training has attempted to standardize the undergraduate and graduate medical education goals. However, it remains unclear as to how the EPAs correlate to the milestones, and who owns the process of ensuring that an entering EM resident has competency at a certain minimum level. Recent trends establishing specialty-specific boot camps prepare students for residency and address the variability of skills of students coming from different medical schools. Objective: Our project’s goal was therefore to perform a needs assessment to inform the design of an EM boot camp curriculum. Toward this goal, we 1 mapped the core EPAs for graduating medical students to the EM residency Level 1 milestones in order to identify the possible gaps/needs and 2 conducted a pilot procedure workshop that was designed to address some of the identified gaps/needs in procedural skills. Methods: In order to inform the curriculum of an EM boot camp, we used a systematic approach to 1 identify gaps between the EPAs and EM milestones (Level 1 and 2 determine what essential and supplemental competencies/skills an incoming EM resident should ideally possess. We then piloted a 1-day, three-station advanced ABCs procedure workshop based on the identified needs. A pre-workshop test and survey assessed knowledge, preparedness, confidence, and perceived competence. A post-workshop survey evaluated the program, and a posttest combined with psychomotor skills test using three

  12. An improved flow analysis-ion chromatography method for determination of cationic and anionic species at trace levels in Antarctic ice cores.

    Science.gov (United States)

    Morganti, Andrea; Becagli, Silvia; Castellano, Emiliano; Severi, Mirko; Traversi, Rita; Udisti, Roberto

    2007-11-12

    A method was developed for the quantitative determination of cations and anions in Antarctic ice cores at microgL(-1) and sub-microgL(-1) levels by ion chromatography (IC), after ultra-clean decontamination procedures. Strict manipulation and decontamination procedures were used in sub-sampling, in order to minimise sample contamination. Na+, NH4+, K+, Mg2+ and Ca2+ were determined by 12-min isocratic elution (H2SO4 eluent). Contemporaneously, in a parallel device, F-, MSA (methanesulfonic acid), Cl-, NO3- and SO4(2-) were analysed in a single 12-min run with multiple-step elution using Na2CO3/NaHCO3 as eluent. Melted ice samples were pumped from their still-closed containers (polystyrene accuvettes with polyethylene caps), shared between the two ion chromatographic systems, online filtered (0.45 microm Teflon membrane) and pre-concentrated (anions and cations pre-concentration columns) using a flow analysis system, thus avoiding uptake of contaminants from the laboratory atmosphere. Sensitivity, linear range, reproducibility and detection limit were evaluated for each chemical species. Anion or cation detection limits ranged from 0.01 to 0.15 microgL(-1) by using a relatively small sample volume (1.5 mL). Such values are significantly lower than those reported in literature for almost all the components. These methods were successfully applied to the analysis of cations and anions at trace levels in the Dome C ice core. The composition of the atmospheric aerosol for the last 850 kyr was reconstructed by high-resolution continuous chemical stratigraphies. Concentration trends in the last nine glacial-interglacial climatic cycles were shown and briefly discussed.

  13. Determination of lead isotopes in a new Greenland deep ice core at the sub-picogram per gram level by thermal ionization mass spectrometry using an improved decontamination method.

    Science.gov (United States)

    Han, Changhee; Burn-Nunes, Laurie J; Lee, Khanghyun; Chang, Chaewon; Kang, Jung-Ho; Han, Yeongcheol; Hur, Soon Do; Hong, Sungmin

    2015-08-01

    An improved decontamination method and ultraclean analytical procedures have been developed to minimize Pb contamination of processed glacial ice cores and to achieve reliable determination of Pb isotopes in North Greenland Eemian Ice Drilling (NEEM) deep ice core sections with concentrations at the sub-picogram per gram level. A PL-7 (Fuso Chemical) silica-gel activator has replaced the previously used colloidal silica activator produced by Merck and has been shown to provide sufficiently enhanced ion beam intensity for Pb isotope analysis for a few tens of picograms of Pb. Considering the quantities of Pb contained in the NEEM Greenland ice core and a sample weight of 10 g used for the analysis, the blank contribution from the sample treatment was observed to be negligible. The decontamination and analysis of the artificial ice cores and selected NEEM Greenland ice core sections confirmed the cleanliness and effectiveness of the overall analytical process.

  14. Measurement of the spin-forbidden decay rate (3s3d)1D2¿(3s3p)3 P2,1 in 24Mg

    DEFF Research Database (Denmark)

    Therkildsen, K. T.; Jensen, Brian Bak; Ryder, C. P.;

    2009-01-01

    We have measured the spin-forbidden decay rate from (3s3d)D12¿(3s3p)P32,1 in M24g atoms trapped in a magneto-optical trap. The total decay rate, summing up both exit channels (3s3p)P31 and (3s3p)P32 , yields 196±10s-1 in excellent agreement with resent relativistic many-body calculations of Porsev...

  15. Ice cores

    DEFF Research Database (Denmark)

    Svensson, Anders

    2014-01-01

    Ice cores from Antarctica, from Greenland, and from a number of smaller glaciers around the world yield a wealth of information on past climates and environments. Ice cores offer unique records on past temperatures, atmospheric composition (including greenhouse gases), volcanism, solar activity......, dustiness, and biomass burning, among others. In Antarctica, ice cores extend back more than 800,000 years before present (Jouzel et al. 2007), whereas. Greenland ice cores cover the last 130,000 years...

  16. Radiative rates for E1, E2, M1, and M2 transitions among the 3s$^2$3p$^5$, 3s3p$^6$, and 3s$^2$3p$^4$3d configurations of Cl-like W LVIII

    CERN Document Server

    Aggarwal, K M

    2014-01-01

    We report calculations of energy levels, radiative decay rates, and lifetimes for transitions among the 3s$^2$3p$^5$, 3s3p$^6$, and 3s$^2$3p$^4$3d configurations of Cl-like W LVIII. The general-purpose relativistic atomic structure package (GRASP) has been adopted for our calculations. Comparisons are made with the most recent results of Mohan et al. [Can. J. Phys. {\\bf 92} (2014) xxx] and discrepancies in lifetimes are noted, up to four orders of magnitude in some instances. Our energy levels are estimated to be accurate to better than 0.5\\%, whereas results for radiative rates and lifetimes should be accurate to better than 20\\%.

  17. Intersubband optical absorption between multi energy levels of electrons in InGaN/GaN spherical core-shell quantum dots

    Science.gov (United States)

    Liu, W. H.; Qu, Y.; Ban, S. L.

    2017-02-01

    The intersubband optical absorption between multi energy levels of electrons in InxGa1-xN/GaN spherical core-shell quantum dots (CSQDs) and ternary mixed crystal and size effects have been investigated by using the principle of density matrix. Electronic eigenstates under the effect of built-in electric field (BEF) have been calculated by a finite element method. The results show that optical absorption between intersubbands with main quantum numbers n = 1 and n = 2 are as important as that between ones with n = 1 and different angular quantum numbers when the BEF is taken into account. In consideration of BEF, the saturation of total optical absorption coefficients (ACs) and secondary peaks of refractive index changes (RICs) appear when incident light intensity I surpasses a certain value. For a given I, the maximum ACs and zero RICs positions in InxGa1-xN/GaN CSQDs with a fixed shell size have a blue-shift when x increases or the core InxGa1-xN radius R1 decreases from 5 nm. However, when R1 > 5 nm, ACs and RICs tend to be stable. The results indicate that effective adjustment of ACs and RICs in CSQDs with BEFs by size is in a limited scale range. The saturation of ACs or secondary peaks of RICs appear more likely in CSQDs with smaller x or larger R1. These results are expected to be helpful both in the further theoretical and experimental study on optic devices consisting of CSQDs.

  18. miR-187-3p inhibits the metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by targeting S100A4.

    Science.gov (United States)

    Dou, Changwei; Liu, Zhikui; Xu, Meng; Jia, Yuli; Wang, Yufeng; Li, Qing; Yang, Wei; Zheng, Xin; Tu, Kangsheng; Liu, Qingguang

    2016-10-28

    miR-187-3p, a novel cancer-related microRNA, was previously reported to play promoting or suppressive roles in different malignancies. However, the expression level, biological function, and underlying mechanisms of miR-187-3p in hepatocellular carcinoma (HCC) remain unknown. This study demonstrated that miR-187-3p was significantly down-regulated in HCC tissues and cell lines, and was associated with advanced TNM stage and metastasis in HCC. Functional studies confirmed that miR-187-3p could inhibit the metastasis of HCC both in vitro and in vivo. Moreover, we proved that miR-187-3p could prevent the epithelial-mesenchymal transition (EMT) of HCC cells. Mechanically, S100A4 was a direct downstream target of miR-187-3p, and mediated the functional influence of miR-187-3p in HCC. Furthermore, miR-187-3p and S100A4 expression was evidently correlated with adverse clinical features and poor prognosis of HCC. Lastly, we showed that hypoxia was responsible for the significantly decreased level of miR-187-3p in HCC, and miR-187-3p was involved in the promoting effects of hypoxia on the metastasis and EMT of HCC cells. Taken together, miR-187-3p inhibits the metastasis and EMT in HCC by targeting S100A4. miR-187-3p can serve as a prognostic indicator and a promising therapeutic target for HCC patients.

  19. Tretinoin-loaded lipid-core nanocapsules decrease reactive oxygen species levels and improve bovine embryonic development during in vitro oocyte maturation.

    Science.gov (United States)

    Lucas, Caroline Gomes; Remião, Mariana Härter; Komninou, Eliza Rossi; Domingues, William Borges; Haas, Cristina; Leon, Priscila Marques Moura de; Campos, Vinicius Farias; Ourique, Aline; Guterres, Silvia S; Pohlmann, Adriana R; Basso, Andrea Cristina; Seixas, Fabiana Kömmling; Beck, Ruy Carlos Ruver; Collares, Tiago

    2015-12-01

    In vitro oocyte maturation (IVM) protocols can be improved by adding chemical supplements to the culture media. Tretinoin is considered an important retinoid in embryonic development and its association with lipid-core nanocapsules (TTN-LNC) represents an innovative way of improving its solubility, and chemical stability, and reducing its toxicity. The effects of supplementing IVM medium with TTN-LNC was evaluated by analyzing production of reactive oxygen species (ROS), S36-phosphorilated-p66Shc levels and caspase activity in early embryonic development, and expression of apoptosis and pluripotency genes in blastocysts. The lowest concentration tested (0.25μM) of TTN-LNC generated higher blastocyst rate, lower ROS production and S36-p66Shc amount. Additionally, expression of BAX and SHC1 were lower in both non-encapsulated tretinoin (TTN) and TTN-LNC-treated groups. Nanoencapsulation allowed the use of smaller concentrations of tretinoin to supplement IVM medium thus reducing toxic effects related with its use, decreasing ROS levels and apoptose frequency, and improving the blastocyst rates.

  20. Luteolin Inhibits Ischemia/Reperfusion-Induced Myocardial Injury in Rats via Downregulation of microRNA-208b-3p.

    Directory of Open Access Journals (Sweden)

    Chen Bian

    Full Text Available Luteolin (LUT, a kind of flavonoid which is extracted from a variety of diets, has been reported to convey protective effects of various diseases. Recent researches have suggested that LUT can carry out cardioprotective effects during ischemia/reperfusion (I/R. However, there have no reports on whether LUT can exert protective effects against myocardial I/R injury through the actions of specific microRNAs (miRs. The purpose of this study was to determine which miRs and target genes LUT exerted such function through.Expression of various miRs in perfused rat hearts was detected using a gene chip. Target genes were predicted with TargetScan, MiRDB and MiRanda. Anoxia/reoxygenation was used to simulate I/R. Cells were transfected by miR-208b-3p mimic, inhibitor and small interfering RNA of Ets1 (avian erythroblastosis virus E26 (v ets oncogene homolog 1. MiR-208b-3p and Ets1 mRNA were quantified by real-time quantitative polymerase chain reaction. The percentage of apoptotic cells was detected by annexin V-fluorescein isothiocyanate/propidium iodide dyeing and flow cytometry. The protein expression levels of cleaved caspase-3, Bcl-2, Bax, and Ets1 were examined by western blot analysis. A luciferase reporter assay was used to verify the combination between miR-208b-3p and the 3'-untranslated region of Ets1.LUT pretreatment reduced miR-208b-3p expression in myocardial tissue, as compared to the I/R group. And LUT decreased miR-208b-3p expression and apoptosis caused by I/R. However, overexpression of miR-208b-3p further aggravated the changes caused by I/R and blocked all the effects of LUT. Knockdown of miR-208b-3p expression also attenuated apoptosis, while knockdown of Ets1 promoted apoptosis. Further, the luciferase reporter assay showed that miR-208b-3p could inhibit Ets1 expression.LUT pretreatment conveys anti-apoptotic effects after myocardial I/R injury by decreasing miR-208b-3p and increasing Ets1 expression levels.

  1. Mechanisms and Kinetics of Radical Reaction of O(1D,3P) + HCN System

    Institute of Scientific and Technical Information of China (English)

    HUANG Yu-Cheng; DU Jin-Yan; JU Xue-Hai; YE Shi-Yong; ZHOU Tao

    2008-01-01

    The reaction of HCN with O(1D, 3P) radical has been investigated by density functional theory (DFT) and ab initio methods. The stationary points on the reaction paths(reactants, intermediates and products) were optimized at the (U)B3LYP/aug-cc-pVTZ level.Single-point calculations were performed at the (U)QCISD(T)/aug-cc-pVTZ level for the optimized structures and all the total energies were corrected by zero-point energy. It is shown that there exist three competing mechanisms of oxygen attacking nitrogen O→N, oxygen attacking carbon O→C and oxygen attacking hydrogen O→H. The rate constants were obtained via Eyring transition-state theory in the temperature range of 600~2000 K. The linear relationship between lnk and 1/T was presented. The results show that path 1 is the main reaction channel and the product of NCO + H is predominant.

  2. Combination of MiR-103a-3p and Mesothelin Improves the Biomarker Performance of Malignant Mesothelioma Diagnosis

    Science.gov (United States)

    Bryk, Oleksandr; Raiko, Irina; Pesch, Beate; Kollmeier, Jens; Bauer, Torsten T.; Brüning, Thomas

    2014-01-01

    Background For the detection of malignant mesothelioma no single biomarker with reasonable sensitivity and specificity has been described so far. Mesothelin, the most prominent blood-based biomarker, is characterized by high specificity but low sensitivity. It might be reasonable to combine biomarkers of different molecular classes in order to improve the overall performance. The aim of this study was to assess the performance of the combination of mesothelin and miR-103a-3p as blood-based biomarker for mesothelioma. Methods/Principal Findings Mesothelin concentration in plasma and miR-103a-3p levels in the cellular blood fraction were analyzed in 43 male mesothelioma patients and 52 male controls formerly exposed to asbestos. For the discrimination of epithelioid and biphasic mesothelioma from asbestos-exposed controls mesothelin and miR-103a-3p showed 74% and 89% sensitivity and 85% and 63% specificity, respectively. For the combination of mesothelin and miR-103a-3p a sensitivity of 95% and a specificity of 81% were calculated. Conclusions/Significance The results of this study show that the combination of mesothelin and miR-103a-3p improves the diagnostic performance of individual blood-based biomarker to detect malignant mesothelioma. The obtained results indicate that the use of biomarkers of different molecular classes might be a reasonable approach to assemble a biomarker panel. PMID:25469901

  3. Combination of MiR-103a-3p and mesothelin improves the biomarker performance of malignant mesothelioma diagnosis.

    Directory of Open Access Journals (Sweden)

    Daniel G Weber

    Full Text Available For the detection of malignant mesothelioma no single biomarker with reasonable sensitivity and specificity has been described so far. Mesothelin, the most prominent blood-based biomarker, is characterized by high specificity but low sensitivity. It might be reasonable to combine biomarkers of different molecular classes in order to improve the overall performance. The aim of this study was to assess the performance of the combination of mesothelin and miR-103a-3p as blood-based biomarker for mesothelioma.Mesothelin concentration in plasma and miR-103a-3p levels in the cellular blood fraction were analyzed in 43 male mesothelioma patients and 52 male controls formerly exposed to asbestos. For the discrimination of epithelioid and biphasic mesothelioma from asbestos-exposed controls mesothelin and miR-103a-3p showed 74% and 89% sensitivity and 85% and 63% specificity, respectively. For the combination of mesothelin and miR-103a-3p a sensitivity of 95% and a specificity of 81% were calculated.The results of this study show that the combination of mesothelin and miR-103a-3p improves the diagnostic performance of individual blood-based biomarker to detect malignant mesothelioma. The obtained results indicate that the use of biomarkers of different molecular classes might be a reasonable approach to assemble a biomarker panel.

  4. IGCSE core mathematics

    CERN Document Server

    Wall, Terry

    2013-01-01

    Give your core level students the support and framework they require to get their best grades with this book dedicated to the core level content of the revised syllabus and written specifically to ensure a more appropriate pace. This title has been written for Core content of the revised Cambridge IGCSE Mathematics (0580) syllabus for first teaching from 2013. ? Gives students the practice they require to deepen their understanding through plenty of practice questions. ? Consolidates learning with unique digital resources on the CD, included free with every book. We are working with Cambridge

  5. Manifestation of screening effects and A-O covalency in the core level spectra of A site elements in the ABO3 structure of Ca1-xSrxRuO3

    Science.gov (United States)

    Singh, Ravi Shankar; Maiti, Kalobaran

    2007-08-01

    We investigate the evolution of Ca2p and Sr3d core level spectra in Ca1-xSrxRuO3 using photoemission spectroscopy. Core level spectra in this system exhibit multiple features and unusual evolution with the composition and temperatures. Analysis of the core level spectra in conjunction with the band structure results indicates final state effects due to different core hole screening channels. Such screening in the photoemission final states can be attributed to the large A-O covalency in these systems. Changes in the core level spectra with temperature and composition suggest significant modification in A-O (A=Ca/Sr) covalency in Ca-dominated samples, which gradually reduces with the increase in Sr content and becomes insignificant in SrRuO3 . This study thus provides a direct evidence of cation-oxygen covalency and its evolution with temperature, which may be useful in understanding the unusual ground state properties of these materials.

  6. Measurement of the Hyperfine Structure and Isotope Shifts of the 3s23p2 3P2 to 3s3p3 3Do3 Transition in Silicon

    CERN Document Server

    Lee, Siu Au

    2010-01-01

    The hyperfine structure and isotope shifts of the 3s23p2 3P2 to 3s3p3 3Do3 transition in silicon have been measured. The transition at 221.7 nm was studied by laser induced fluorescence in an atomic Si beam. For 29Si, the hyperfine A constant for the 3s23p2 3P2 level was determined to be -160.1+-1.3 MHz (1 sigma error), and the A constant for the 3s3p3 3Do3 level is -532.9+-0.6 MHz. This is the first time that these constants were measured. The isotope shifts (relative to the abundant isotope 28Si) of the transition were determined to be 1753.3+-1.1 MHz for 29Si and 3359.9+-0.6 MHz for 30Si. This is an improvement by about two orders of magnitude over a previous measurement. From these results we are able to predict the hyperfine structure and isotope shift of the radioactive 31Si atom, which is of interest in building a scalable quantum computer.

  7. Interface reactions in Mg/Zn3P2 solar cells

    Science.gov (United States)

    Kazmerski, L. L.; Ireland, P. J.; Catalano, A.

    1981-05-01

    The composition and chemistry of the Mg/Zn3P2 interface was studied using surface analysis techniques. For higher carrier concentrations (p 10 to the 17th power/cu cm), a heterojunction occurs by the chemical interaction of the Mg with Zn3P2 to form Mg3P2. For lower carrier concentrations this reaction does not take place, and Mg diffuses into the Zn3P2. Diffusion coefficients of Mg in Zn3P2 are determined. Grain (crystalline) and grain boundary components of the diffusion coefficient are evaluated. These data compare very well with that reported using electron beam induced currents and spectral response measurements.

  8. Role of miR-222-3p in c-Src-Mediated Regulation of Osteoclastogenesis

    Directory of Open Access Journals (Sweden)

    Shinya Takigawa

    2016-02-01

    Full Text Available MicroRNAs (miRNAs are small non-coding RNAs that play a mostly post-transcriptional regulatory role in gene expression. Using RAW264.7 pre-osteoclast cells and genome-wide expression analysis, we identified a set of miRNAs that are involved in osteoclastogenesis. Based on in silico analysis, we specifically focused on miR-222-3p and evaluated its role in osteoclastogenesis. The results show that the inhibitor of miR-222-3p upregulated the mRNA levels of nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1 and tartrate-resistant acid phosphatase (TRAP, while its mimicking agent downregulated their mRNA levels. Western blot analysis showed that its inhibitor increased the protein levels of TRAP and cathepsin K, while its mimicking agent decreased their levels. Genome-wide mRNA expression analysis in the presence and absence of receptor activator of nuclear factor κ-B ligand (RANKL predicted c-Src as a potential regulatory target of miR-222-3p. Live cell imaging using a fluorescence resonance energy transfer (FRET technique revealed that miR-222-3p acted as an inhibitor of c-Src activity, and a partial silencing of c-Src suppressed RANKL-induced expression of TRAP and cathepsin K, as well as the number of multi-nucleated osteoclasts and their pit formation. Collectively, the study herein demonstrates that miR-222-3p serves as an inhibitor of osteoclastogenesis and c-Src mediates its inhibition of cathepsin K and TRAP.

  9. Response of MiRNA-22-3p and MiRNA-149-5p to Folate Deficiency and the Differential Regulation of MTHFR Expression in Normal and Cancerous Human Hepatocytes

    Science.gov (United States)

    Li, Chao; Ni, Juan; Liu, Yao-Xian; Wang, Han; Liang, Zi-Qing; Wang, Xu

    2017-01-01

    Background/Aims Folic acid (FA) is a core micronutrient involved in DNA synthesis/methylation, and the metabolism of FA is responsible for genomic stability. MicroRNAs may affect gene expression during folate metabolism when cellular homeostasis is changed. This study aimed to reveal the relationship between FA deficiency and the expression of miR-22-p/miR-149-5p and the targeted regulation of miR-22-3p/miR-149-5p on the key folate metabolic gene Methylenetetrahydrofolate reductase (MTHFR). Methods Normal (HL-7702 cells) and cancerous (QGY-7703 cells) human hepatocytes were intervened in modified RPMI 1640 with FA deficiency for 21 days. The interaction between MTHFR and the tested miRNAs was verified by Dual-Luciferase Reporter Assays. The changes in the expression of miR-22-3p/miR-149-5p in response to FA deficiency were detected by Poly (A) Tailing RT-qPCR, and the expression of MTHFR at both the transcriptional and translational levels was determined by RT-qPCR and Western blotting, respectively. Result MiR-22-3p/miR-149-5p directly targeted the 3’UTR sequence of the MTHFR gene. FA deficiency led to an upregulation of miR-22-3p/miR-149-5p expression in QGY-7703/HL-7702 cells, while the transcription of MTHFR was decreased in QGY-7703 cells but elevated in HL-7702 cells. Western blotting showed that FA deficiency resulted in a decline of the MTHFR protein in QGY-7703 cells, whereas in HL-7702 cells, the MTHFR protein level remained constant. Conclusion The results suggested that miR-22-3p/miR-149-5p exert different post-transcriptional effects on MTHFR under conditions of FA deficiency in normal and cancerous human hepatocytes. The results also implied that miR-22-3p/miR-149-5p might exert anticancer effects in cases of long-term FA deficiency. PMID:28045918

  10. RECENT DECLINES IN PAH, PCB, AND TOXAPHENE LEVELS IN THE NORTHERN GREAT LAKES AS DETERMINED FROM HIGH RESOLUTION SEDIMENT CORES. (R825151)

    Science.gov (United States)

    Sediment cores were collected from two sites in Grand Traverse Bay, Lake Michigan in May 1998, dated using 210Pb geochronology, and analyzed for polychlorinated biphenyl (PCB) congeners, polycyclic aromatic hydrocarbons (PAHs), and toxaphene. The extraor...

  11. MicroRNA-219-2-3p functions as a tumor suppressor in gastric cancer and is regulated by DNA methylation.

    Directory of Open Access Journals (Sweden)

    Huizi Lei

    Full Text Available BACKGROUND AIMS: Gastric cancer is the most frequent gastrointestinal tumor in adults and is the most lethal form of human cancer. Despite of the improvements in treatments, the underlying mechanism of gastric carcinogenesis is not well known. To define novel modulators that regulate susceptibility to tumorgenesis, we focused on miR-219-2-3p. METHODS: Quantitative RT-PCR was employed to investigate the level of miR-219-2-3p in gastric cancer (GC tissues (n = 113 and their matched adjacent normal tissues (n = 113. In vitro cell proliferation, apoptosis assays, cell migration, and invasion assays were performed to elucidate biological effects of miR-219-2-3p. Since silencing of miRNA by promoter CpG island methylation may be an important mechanism in tumorgenesis, GC cells were treated with 5-aza-2'-deoxycytidine and trichostatin A, and expression changes of miR-219-2-3p were subsequently examined by quantitative RT-PCR. Finally, the methylation status of CpG island upstream of miR-219-2-3p was analyzed by methylation-specific PCR in GC tissues (n = 22. RESULTS: miR-219-2-3p was down-regulated in GC and cell lines. In addition, the experiments documented the lower expression of miR-219-2-3p in GC specimens with higher grade and later stage tumors. Meanwhile, miR-219-2-3p exerted antiproliferative, proapoptotic, and antimetastatic roles and reduced levels of p-ERK1/2 in GC cells. Furthermore, 5-aza-2'-deoxycytidine and trichostatin A increased the expression (~2 fold of miR-219-2-3p in GC cells. By methylation-specific PCR, DNA methylation in the upstream region of miR-219-2-3p was detected in both adjacent normal tissues and cancer tissues. As expected, the methylation level was considerably higher in the miR-219-2-3p down-regulated group than up-regulated group. CONCLUSIONS: miR-219-2-3p is potentially involved in gastric cancer progression and metastasis by regulating ERK1/2-related signal pathways, which may provide a novel therapeutic strategy

  12. Excited-configuration metastable level lifetimes of Cl-like Mn IX and Fe X

    Science.gov (United States)

    Moehs; Church; Bhatti; Perger

    2000-07-03

    Forbidden transitions from levels with 3P and 1D cores in excited configurations of Cl-like Mn IX and Fe X have been isolated using interference filters. The fluorescence decay lifetimes of ions orbiting in a Kingdon ion trap were measured. New relativistic configuration interaction calculations of relevant level lifetimes, to aid the analysis, based on B-spline basis sets, are also presented. Line identifications and experimental lifetimes are Mn IX ((4)D(7/2)-(4)F(9/2)):363(-3/+7) nm; tau(Mn IX3p(4)(3P)3d (4)F(9/2)) = 210+/-42 ms; tau(Fe X,3p(4)(3P)3d (4)F(9/2)) = 85.7+/-9.2 ms; tau(Fe X,3p(4)(3P)3d (4)F(7/2)) = 93+/-30 ms; and tau(Fe X, 3p(4)(1D)3d (2)G(9/2)) = 17.8+/-3.1 ms.

  13. MiR-466b-1-3p regulates P-glycoprotein expression in rat cerebral microvascular endothelial cells.

    Science.gov (United States)

    Yang, Xiaobo; Ren, Weimin; Shao, Yiye; Chen, Yinghui

    2017-04-03

    Epilepsy is one of the most common neurological disorders, and approximately one-third of epilepsy cases are resistant to treatment with anti-epileptic drug (AED). P-glycoprotein (P-gp) is a multi-drug transporter that is thought to play a pivotal role in multiple drug resistance (MDR) in epilepsy. The regulatory mechanism of P-gp remains largely unknown; however, recent studies have demonstrated that microRNAs (miRNAs) may regulate the chemo-resistance mediated by P-gp. This study investigated the effect of specific miRNAs that regulate P-gp expression in rat cerebral microvascular endothelial cells (RCMECs). Primary cultures of RCMECs were treated with phenobarbital (PB) at various concentrations to induce P-gp overexpression. MiRNA microarrays were used to investigate the expression profiles of miRNAs in the resistant RCMECs induced by PB and corresponding non-resistant cells. Our data demonstrated decreased miR-466b-1-3p expression in the resistant cells compared with the non-resistant cells. Moreover, the recombinant RNA of 466b-1-3p (mimic) and the artificial antisense RNA of miR-466b-1-3p (inhibitor) were constructed and transfected into resistant RCMECs. The expression and function of P-gp were measured by Western blotting, quantitative real-time polymerase chain reaction (qRT-PCR) and flow cytometry using rhodamine efflux. The mRNA and protein levels of P-gp increased as the concentration of PB increased, whereas miR-466b-1-3p levels decreased with increasing PB concentrations (Pp mimic down-regulated P-gp expression, whereas the miR-466b-1-3p inhibitor up-regulated P-gp expression (Pp may regulate PB-induced P-gp expression in RCMECs.

  14. Energetics and Dynamics of the Reactions of O(3P) with Dimethyl Methylphosphonate and Saria

    Science.gov (United States)

    2009-09-15

    a SN2 -like transition geometry, Figure 3c, the axial O-C-H bond angle is slightly bent more for reaction 4, Figure 3d. The products of reaction 4 are...Energetics and Dynamics of the Reactions of O(3P) with Dimethyl Methylphosphonate and Sarin Patrick F. Conforti and Matthew Braunstein* Spectral...calculations were performed on the reaction systems O(3P) + sarin and O(3P) + dimethyl methylphosphonate (DMMP), a sarin simulant. Transition state

  15. Ice Cores

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Records of past temperature, precipitation, atmospheric trace gases, and other aspects of climate and environment derived from ice cores drilled on glaciers and ice...

  16. Core BPEL

    DEFF Research Database (Denmark)

    Hallwyl, Tim; Højsgaard, Espen

    extensions. Combined with the fact that the language definition does not provide a formal semantics, it is an arduous task to work formally with the language (e.g. to give an implementation). In this paper we identify a core subset of the language, called Core BPEL, which has fewer and simpler constructs......, does not allow omissions, and does not contain ignorable elements. We do so by identifying syntactic sugar, including default values, and ignorable elements in WS-BPEL. The analysis results in a translation from the full language to the core subset. Thus, we reduce the effort needed for working...... formally with WS-BPEL, as one, without loss of generality, need only consider the much simpler Core BPEL. This report may also be viewed as an addendum to the WS-BPEL standard specification, which clarifies the WS-BPEL syntax and presents the essential elements of the language in a more concise way...

  17. Ligand field splittings in core level transitions for transition metal (TM) oxides: Tanabe-Sugano diagrams and (TM) dangling bonds in vacated O-atom defects

    Science.gov (United States)

    Lucovsky, Gerry; Wu, Kun; Pappas, Brian; Whitten, Jerry

    2013-04-01

    Defect states in the forbidden band-gap below the conduction band edge are active as electron traps in nano-grain high-) transition metal (TM) oxides with thickness >0.3 nm, e.g., ZrO2 and HfO2. These oxides have received considerable attention as gate-dielectrics in complementary metal oxide semiconductor (CMOS) devices, and more recently are emerging as candidates for charge storage and memory devices. To provide a theoretical basis for device functionality, ab-initio many-electron theory is combined with X-ray absorption spectroscopy (XAS) to study O K edge and TM core level transitions. These studies identify ligand field splittings (ΔLF) for defect state features,. When compared with those obtained from O-atom and TM-atom core spectroscopic transitions, this provides direct information about defect state sun-nm bonding arrangements. comparisons are made for (i) elemental TiO2 and Ti2O3 with different formal ionic charges, Ti4+ and Ti3+ and for (ii) Magneli Phase alloys, TinO2n-1, n is an integer 9>=n>3, and (TiO2)x(HfO2)1-x alloys. The alloys display multi-valent behavior from (i) different ionic-charge states, (ii} local bond-strain, and (iii) metallic hopping transport. The intrinsic bonding defects in TM oxides are identified as pairs of singly occupied dangling bonds. For 6-fold coordinated Ti-oxides defect excited states in 2nd derivative O K pre-edge spectra are essentially the same as single Ti-atom d2 transitions in Tanabe-Sugano (T-S) diagrams. O-vacated site defects in 8-fold coordinated ZrO2 and HfO2 are described by d8 T-S diagrams. T-S defect state ordering and splittings are functions of the coordination and symmetry of vacated site bordering TM atoms. ΔLF values from the analysis of T-S diagrams indicate medium range order (MRO) extending to 3rd and 4th nearest-neighbor (NN) TM-atoms. Values are different for 6-fold Ti, and 8-fold ZrO2 and HfO2, and scale inversely with differences in respective formal ionic radii. O-vacated site bonding

  18. Phospholipid flippases Lem3p-Dnf1p and Lem3p-Dnf2p are involved in the sorting of the tryptophan permease Tat2p in yeast.

    Science.gov (United States)

    Hachiro, Takeru; Yamamoto, Takaharu; Nakano, Kenji; Tanaka, Kazuma

    2013-02-01

    The type 4 P-type ATPases are flippases that generate phospholipid asymmetry in membranes. In budding yeast, heteromeric flippases, including Lem3p-Dnf1p and Lem3p-Dnf2p, translocate phospholipids to the cytoplasmic leaflet of membranes. Here, we report that Lem3p-Dnf1/2p are involved in transport of the tryptophan permease Tat2p to the plasma membrane. The lem3Δ mutant exhibited a tryptophan requirement due to the mislocalization of Tat2p to intracellular membranes. Tat2p was relocalized to the plasma membrane when trans-Golgi network (TGN)-to-endosome transport was inhibited. Inhibition of ubiquitination by mutations in ubiquitination machinery also rerouted Tat2p to the plasma membrane. Lem3p-Dnf1/2p are localized to endosomal/TGN membranes in addition to the plasma membrane. Endocytosis mutants, in which Lem3p-Dnf1/2p are sequestered to the plasma membrane, also exhibited the ubiquitination-dependent missorting of Tat2p. These results suggest that Tat2p is ubiquitinated at the TGN and missorted to the vacuolar pathway in the lem3Δ mutant. The NH(2)-terminal cytoplasmic region of Tat2p containing ubiquitination acceptor lysines interacted with liposomes containing acidic phospholipids, including phosphatidylserine. This interaction was abrogated by alanine substitution mutations in the basic amino acids downstream of the ubiquitination sites. Interestingly, a mutant Tat2p containing these substitutions was missorted in a ubiquitination-dependent manner. We propose the following model based on these results; Tat2p is not ubiquitinated when the NH(2)-terminal region is bound to membrane phospholipids, but if it dissociates from the membrane due to a low level of phosphatidylserine caused by perturbation of phospholipid asymmetry in the lem3Δ mutant, Tat2p is ubiquitinated and then transported from the TGN to the vacuole.

  19. miR-410-3p suppresses breast cancer progression by targeting Snail.

    Science.gov (United States)

    Zhang, Ya-Feng; Yu, Yue; Song, Wang-Zhao; Zhang, Rui-Ming; Jin, Shan; Bai, Jun-Wen; Kang, Hong-Bin; Wang, Xin; Cao, Xu-Chen

    2016-07-01

    miR-410-3p acts as an oncogene or tumor-suppressor gene in various types of cancer. However, its role in breast cancer remains unknown. In the present study, expression of miR-410-3p in 30 breast cancer and paired adjacent normal tissues was detected by RT-qPCR. The expression of miR-410-3p was downregulated in 76.7% of the breast cancer samples. To further validate the expression of miR-410-3p in breast cancer, we analyzed miR-410-3p expression profiling data set from The Cancer Genome Atlas (TCGA) including 683 breast cancer and 87 normal breast tissues. We observed that the expression of miR-410-3p was downregulated in breast cancer tissues. Next, we investigated the influence of miR-410-3p on cell proliferation by transiently transfecting the miR-410-3p mimic or inhibitor, as well as their corresponding controls in the MDA-MB-231 and MCF7 cell lines. miR-410-3p overexpression reduced cell growth, colony formation and the number of EdU-positive cells in the MDA-MB-231 cells. In contrast, inhibition of miR-410-3p in the MCF7 cells resulted in a higher proliferation rate as assessed by MTT assay, plate colony formation and EdU assays. Furthermore, miR-410-3p inhibited epithelial-mesenchymal transition. In addition, Snail was found to be a direct target of miR-410-3p based on a luciferase assay. Overexpression of Snail was able to rescue the effect of miR-410-3p in breast cancer cells. Moreover, miR‑410-3p was inversely expressed with Snail in breast cancer samples. Our data provide new knowledge regarding the role of miR-410-3p in breast cancer progression.

  20. Two PI 3-kinases and one PI 3-phosphatase together establish the cyclic waves of phagosomal PtdIns(3P critical for the degradation of apoptotic cells.

    Directory of Open Access Journals (Sweden)

    Nan Lu

    2012-01-01

    Full Text Available Phosphatidylinositol 3-phosphate (PtdIns(3P is a signaling molecule important for many membrane trafficking events, including phagosome maturation. The level of PtdIns(3P on phagosomes oscillates in two waves during phagosome maturation. However, the physiological significance of such oscillation remains unknown. Currently, the Class III PI 3-kinase (PI3K Vps34 is regarded as the only kinase that produces PtdIns(3P in phagosomal membranes. We report here that, in the nematode C. elegans, the Class II PI3K PIKI-1 plays a novel and crucial role in producing phagosomal PtdIns(3P. PIKI-1 is recruited to extending pseudopods and nascent phagosomes prior to the appearance of PtdIns(3P in a manner dependent on the large GTPase dynamin (DYN-1. PIKI-1 and VPS-34 act in sequence to provide overlapping pools of PtdIns(3P on phagosomes. Inactivating both piki-1 and vps-34 completely abolishes the production of phagosomal PtdIns(3P and disables phagosomes from recruiting multiple essential maturation factors, resulting in a complete arrest of apoptotic-cell degradation. We have further identified MTM-1, a PI 3-phosphatase that antagonizes the activities of PIKI-1 and VPS-34 by down-regulating PtdIns(3P on phagosomes. Remarkably, persistent appearance of phagosomal PtdIns(3P, as a result of inactivating mtm-1, blocks phagosome maturation. Our findings demonstrate that the proper oscillation pattern of PtdIns(3P on phagosomes, programmed by the coordinated activities of two PI3Ks and one PI 3-phosphatase, is critical for phagosome maturation. They further shed light on how the temporally controlled reversible phosphorylation of phosphoinositides regulates the progression of multi-step cellular events.

  1. Sizeable beta-strength in 31Ar (beta 3p) decay

    DEFF Research Database (Denmark)

    T. Koldste, G.; Blank, B.; J. G. Borge, M.

    2014-01-01

    We present for the first time precise spectroscopic information on the recently discovered decay mode beta-delayed 3p-emission. The detection of the 3p events gives an increased sensitivity to the high energy part of the Gamow-Teller strength distribution from the decay of 31Ar revealing that as ...

  2. Terminal 3p deletions in two families--correlation between molecular karyotype and phenotype.

    NARCIS (Netherlands)

    Pohjola, P.; Leeuw, N. de; Penttinen, M.; Kaariainen, H.

    2010-01-01

    The 3p deletion syndrome is a rare disorder caused by deletions of different sizes in the 3p25-pter region. It is characterized by growth retardation, developmental delay, mental retardation, dysmorphism, microcephaly, and ptosis. The phenotype of individuals with deletions varies from normal to sev

  3. Up-regulation of miR-95-3p in hepatocellular carcinoma promotes tumorigenesis by targeting p21 expression

    Science.gov (United States)

    Ye, Jian; Yao, Yufeng; Song, Qixue; Li, Sisi; Hu, Zhenkun; Yu, Yubing; Hu, Changqing; Da, Xingwen; Li, Hui; Chen, Qiuyun; Wang, Qing K.

    2016-01-01

    Hepatocellular carcinoma (HCC) is one of the most common malignant cancers. To elucidate new regulatory mechanisms for heptocarcinogenesis, we investigated the regulation of p21, a cyclin-dependent kinase (CDK) inhibitor encoded by CDKN1A, in HCC. The expression level of p21 is decreased with the progression of HCC. Luciferase assays with a luciferase-p21-3′ UTR reporter and its serial deletions identified a 15-bp repressor element at the 3′-UTR of CDKN1A, which contains a binding site for miR-95-3p. Mutation of the binding site eliminated the regulatory effect of miR-95-3p on p21 expression. Posttranscriptional regulation of p21 expression by miR-95-3p is mainly on the protein level (suppression of translation). Overexpression of miR-95-3p in two different HCC cell lines, HepG2 and SMMC7721, significantly promoted cell proliferation, cell cycle progression and cell migration, whereas a miR-95-3p specific inhibitor decreased cell proliferation, cell cycle progression and cell migration. The effects of miR-95-3p on cellular functions were rescued by overexpression of p21. Overexpression of miR-95-3p promoted cell proliferation and tumor growth in HCC xenograft mouse models. Expression of miR-95-3p was significantly higher in HCC samples than in adjacent non-cancerous samples. These results demonstrate that miR-95-3p is a potential new marker for HCC and regulates hepatocarcinogenesis by directly targeting CDKN1A/p21 expression. PMID:27698442

  4. MiR-338-3p inhibits hepatocarcinoma cells and sensitizes these cells to sorafenib by targeting hypoxia-induced factor 1α.

    Directory of Open Access Journals (Sweden)

    Haitao Xu

    Full Text Available Hypoxia is a common feature of solid tumors and an important contributor to anti-tumor drug resistance. Hypoxia inducible factor-1 (HIF-1 is one of the key mediators of the hypoxia signaling pathway, and was recently proven to be required for sorafenib resistance in hepatocarcinoma (HCC. MicroRNAs have emerged as important posttranslational regulators in HCC. It was reported that miR-338-3p levels are associated with clinical aggressiveness of HCC. However, the roles of miR-338-3p in HCC disease and resistance to its therapeutic drugs are unknown. In this study, we found that miR-338-3p was frequently down-regulated in 14 HCC clinical samples and five cell lines. Overexpression of miR-338-3p inhibited HIF-1α 3'-UTR luciferase activity and HIF-1α protein levels in HepG2, SMMC-7721, and Huh7 cells. miR-338-3p significantly reduced cell viability and induced cell apoptosis of HCC cells. Additionally, HIF-1α overexpression rescued and HIF-1α knock-down abrogated the anti-HCC activity of miR-338-3p. Furthermore, miR-338-3p sensitized HCC cells to sorafenib in vitro and in a HCC subcutaneous nude mice tumor model by inhibiting HIF-1α. Collectively, miR-338-3p inhibits HCC tumor growth and sensitizes HCC cells to sorafenib by down-regulating HIF-1α. Our data indicate that miR-338-3p could be a potential candidate for HCC therapeutics.

  5. Palynological record of tropical rain forest vegetation and sea level fluctuations since 140 ka from sediment core, south-eastern Arabian sea.

    Digital Repository Service at National Institute of Oceanography (India)

    Farooqui, A.; Pattan, J.N.; Parthiban, G.; Srivastava, J.; Ranjana

    to the present day flora in Kerala except Ongokea gore which is now present in South Africa and therefore, the provenance of pollen recorded in the studied core is Western Ghats of India. High percentage of planktic foraminifera and dinoflagellate cysts...

  6. The direct measurement of the 3 3P0-3 3P1 fine-structure interval and the gJ-factor of atomic silicon by laser magnetic resonance

    Science.gov (United States)

    Evenson, K. M.; Beltran-Lopez, V.; Ley-Koo, E.; Inguscio, M.

    1984-01-01

    The J - 1 fine structure interval and the g-factor of the 3P1 state have been determined with high precision in the present laser magnetic resonance measurements of the ground 3p2 3P multiplet of atomic Si. Delta-E(3P1-3P0) = 2,311,755.6(7) MHz, and gJ(3P1) = 1.500830(70). Single-configuration calculations of gJ for 3P1 and 3P2 yield a value for the latter which, at 1.501095, is noted to differ by an unexpectedly large margin from the experimental value.

  7. MicroRNAs miR-203-3p, miR-664-3p and miR-708-5p are associated with median strain lifespan in mice

    Science.gov (United States)

    Lee, Benjamin P.; Burić, Ivana; George-Pandeth, Anupriya; Flurkey, Kevin; Harrison, David E.; Yuan, Rong; Peters, Luanne L.; Kuchel, George A.; Melzer, David; Harries, Lorna W.

    2017-01-01

    MicroRNAs (miRNAs) are small non-coding RNA species that have been shown to have roles in multiple processes that occur in higher eukaryotes. They act by binding to specific sequences in the 3’ untranslated region of their target genes and causing the transcripts to be degraded by the RNA-induced silencing complex (RISC). MicroRNAs have previously been reported to demonstrate altered expression in several aging phenotypes such as cellular senescence and age itself. Here, we have measured the expression levels of 521 small regulatory microRNAs (miRNAs) in spleen tissue from young and old animals of 6 mouse strains with different median strain lifespans by quantitative real-time PCR. Expression levels of 3 microRNAs were robustly associated with strain lifespan, after correction for multiple statistical testing (miR-203-3p [β-coefficient = −0.6447, p = 4.8 × 10−11], miR-664-3p [β-coefficient = 0.5552, p = 5.1 × 10−8] and miR-708-5p [β-coefficient = 0.4986, p = 1.6 × 10−6]). Pathway analysis of binding sites for these three microRNAs revealed enrichment of target genes involved in key aging and longevity pathways including mTOR, FOXO and MAPK, most of which also demonstrated associations with longevity. Our results suggests that miR-203-3p, miR-664-3p and miR-708-5p may be implicated in pathways determining lifespan in mammals. PMID:28304372

  8. A Feedback Regulatory Loop between G3P and Lipid Transfer Proteins DIR1 and AZI1 Mediates Azelaic-Acid-Induced Systemic Immunity

    Directory of Open Access Journals (Sweden)

    Keshun Yu

    2013-04-01

    Full Text Available Systemic acquired resistance (SAR, a highly desirable form of plant defense, provides broad-spectrum immunity against diverse pathogens. The recent identification of seemingly unrelated chemical inducers of SAR warrants an investigation of their mutual interrelationships. We show that SAR induced by the dicarboxylic acid azelaic acid (AA requires the phosphorylated sugar derivative glycerol-3-phosphate (G3P. Pathogen inoculation induced the release of free unsaturated fatty acids (FAs and thereby triggered AA accumulation, because these FAs serve as precursors for AA. AA accumulation in turn increased the levels of G3P, which is required for AA-conferred SAR. The lipid transfer proteins DIR1 and AZI1, both of which are required for G3P- and AA-induced SAR, were essential for G3P accumulation. Conversely, reduced G3P resulted in decreased AZI1 and DIR1 transcription. Our results demonstrate that an intricate feedback regulatory loop among G3P, DIR1, and AZI1 regulates SAR and that AA functions upstream of G3P in this pathway.

  9. A software platform to analyse the ethical issues of electronic patient privacy policy: the S3P example.

    Science.gov (United States)

    Mizani, M A; Baykal, N

    2007-12-01

    Paper-based privacy policies fail to resolve the new changes posed by electronic healthcare. Protecting patient privacy through electronic systems has become a serious concern and is the subject of several recent studies. The shift towards an electronic privacy policy introduces new ethical challenges that cannot be solved merely by technical measures. Structured Patient Privacy Policy (S3P) is a software tool assuming an automated electronic privacy policy in an electronic healthcare setting. It is designed to simulate different access levels and rights of various professionals involved in healthcare in order to assess the emerging ethical problems. The authors discuss ethical issues concerning electronic patient privacy policies that have become apparent during the development and application of S3P.

  10. Electronic and Rovibrational Quantum Chemical Analysis of C$_3$P$^-$: The Next Interstellar Anion?

    CERN Document Server

    Fortenberry, Ryan C

    2015-01-01

    C$_3$P$^-$ is analogous to the known interstellar anion C$_3$N$^-$ with phosphorus replacing the nitrogen in a simple step down the periodic table. In this work, it is shown that C$_3$P$^-$ is likely to possess a dipole-bound excited state. It has been hypothesized and observationally supported that dipole-bound excited states are an avenue through which anions could be formed in the interstellar medium. Additionally, C$_3$P$^-$ has a valence excited state that may lead to further stabilization of this molecule, and C$_3$P$^-$ has a larger dipole moment than neutral C$_3$P ($\\sim 6$ D vs. $\\sim 4$ D). As such, C$_3$P$^-$ is probably a more detectable astromolecule than even its corresponding neutral radical. Highly-accurate quantum chemical quartic force fields are also applied to C$_3$P$^-$ and its singly $^{13}$C substituted isotopologues in order to provide structures, vibrational frequencies, and spectroscopic constants that may aid in its detection.

  11. Orientation of Zn3P2 films via phosphidation of Zn precursors

    Science.gov (United States)

    Katsube, Ryoji; Nose, Yoshitaro

    2017-02-01

    Orientation of solar absorber is an important factor to achieve high efficiency of thin film solar cells. In the case of Zn3P2 which is a promising absorber of low-cost and high-efficiency solar cells, (110)/(001) orientation was only reported in previous studies. We have successfully prepared (101)-oriented Zn3P2 films by phosphidation of (0001)-oriented Zn films at 350 °C. The phosphidation mechanism of Zn is discussed through STEM observations on the partially-reacted sample and the consideration of the relationship between the crystal structures of Zn and Zn3P2 . We revealed that (0001)-oriented Zn led to nucleation of (101)-oriented Zn3P2 due to the similarity in atomic arrangement between Zn and Zn3P2 . The electrical resistivity of the (101)-oriented Zn3P2 film was lower than those of (110)/(001)-oriented films, which is an advantage of the phosphidation technique to the growth processes in previous works. The results in this study demonstrated that well-conductive Zn3P2 films could be obtained by controlling orientations of crystal grains, and provide a guiding principle for microstructure control in absorber materials.

  12. Isotopic and hydrologic responses of small, closed lakes to climate variability: Comparison of measured and modeled lake level and sediment core oxygen isotope records

    Science.gov (United States)

    Steinman, Byron A.; Abbott, Mark B.; Nelson, Daniel B.; Stansell, Nathan D.; Finney, Bruce P.; Bain, Daniel J.; Rosenmeier, Michael F.

    2013-03-01

    Simulations conducted using a coupled lake-catchment isotope mass balance model forced with continuous precipitation, temperature, and relative humidity data successfully reproduce (within uncertainty limits) long-term (i.e., multidecadal) trends in reconstructed lake surface elevations and sediment core oxygen isotope (δ18O) values at Castor Lake and Scanlon Lake, north-central Washington. Error inherent in sediment core dating methods and uncertainty in climate data contribute to differences in model reconstructed and measured short-term (i.e., sub-decadal) sediment (i.e., endogenic and/or biogenic carbonate) δ18O values, suggesting that model isotopic performance over sub-decadal time periods cannot be successfully investigated without better constrained climate data and sediment core chronologies. Model reconstructions of past lake surface elevations are consistent with estimates obtained from aerial photography. Simulation results suggest that precipitation is the strongest control on lake isotopic and hydrologic dynamics, with secondary influence by temperature and relative humidity. This model validation exercise demonstrates that lake-catchment oxygen isotope mass balance models forced with instrumental climate data can reproduce lake hydrologic and isotopic variability over multidecadal (or longer) timescales, and therefore, that such models could potentially be used for quantitative investigations of paleo-lake responses to hydroclimatic change.

  13. Sizeable beta-strength in {sup 31}Ar (β3p) decay

    Energy Technology Data Exchange (ETDEWEB)

    Koldste, G.T. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Blank, B. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Borge, M.J.G.; Briz, J.A.; Carmona-Gallardo, M. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Fraile, L.M. [Grupo de Física Nuclear, Universidad Complutense, CEI Moncloa, E-28040 Madrid (Spain); Fynbo, H.O.U. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Giovinazzo, J. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Johansen, J.G. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Jokinen, A. [Department of Physics, University of Jyväskylä, FIN-40351 Jyväskylä (Finland); Jonson, B. [Fundamental Fysik, Chalmers Tekniska Högskola, S-41296 Göteborg (Sweden); Kurturkian-Nieto, T. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Nilsson, T. [Fundamental Fysik, Chalmers Tekniska Högskola, S-41296 Göteborg (Sweden); Perea, A.; Pesudo, V. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); and others

    2014-10-07

    We present for the first time precise spectroscopic information on the recently discovered decay mode β-delayed 3p-emission. The detection of the 3p events gives an increased sensitivity to the high energy part of the Gamow–Teller strength distribution from the decay of {sup 31}Ar revealing that as much as 30% of the strength resides in the β3p-decay mode. A simplified description of how the main decay modes evolve as the excitation energy increases in {sup 31}Cl is provided.

  14. Progress on the Multiphysics Capabilities of the Parallel Electromagnetic ACE3P Simulation Suite

    Energy Technology Data Exchange (ETDEWEB)

    Kononenko, Oleksiy [SLAC National Accelerator Lab., Menlo Park, CA (United States)

    2015-03-26

    ACE3P is a 3D parallel simulation suite that is being developed at SLAC National Accelerator Laboratory. Effectively utilizing supercomputer resources, ACE3P has become a key tool for the coupled electromagnetic, thermal and mechanical research and design of particle accelerators. Based on the existing finite-element infrastructure, a massively parallel eigensolver is developed for modal analysis of mechanical structures. It complements a set of the multiphysics tools in ACE3P and, in particular, can be used for the comprehensive study of microphonics in accelerating cavities ensuring the operational reliability of a particle accelerator.

  15. Programming of adipose tissue miR-483-3p and GDF-3 expression by maternal diet in type 2 diabetes

    DEFF Research Database (Denmark)

    Ferland-McCollough, D; Fernandez-Twinn, D S; Cannell, I G

    2012-01-01

    Nutrition during early mammalian development permanently influences health of the adult, including increasing the risk of type 2 diabetes and coronary heart disease. However, the molecular mechanisms underlying such programming are poorly defined. Here we demonstrate that programmed changes in miRNA...... expression link early-life nutrition to long-term health. Specifically, we show that miR-483-3p is upregulated in adipose tissue from low-birth-weight adult humans and prediabetic adult rats exposed to suboptimal nutrition in early life. We demonstrate that manipulation of miR-483-3p levels in vitro...... substantially modulates the capacity of adipocytes to differentiate and store lipids. We show that some of these effects are mediated by translational repression of growth/differentiation factor-3, a target of miR-483-3p. We propose that increased miR-483-3p expression in vivo, programmed by early...

  16. Dynamics of the gas-liquid interfacial reaction of O(3P) atoms with hydrocarbons

    Science.gov (United States)

    Kelso, Hailey; Köhler, Sven P. K.; Henderson, David A.; McKendrick, Kenneth G.

    2003-11-01

    We describe an experimental approach to the determination of the nascent internal state distribution of gas-phase products of a gas-liquid interfacial reaction. The system chosen for study is O(3P) atoms with the surface of liquid deuterated squalane, a partially branched long-chain saturated hydrocarbon, C30D62. The nascent OD products are detected by laser-induced fluorescence. Both OD (v'=0) and (v'=1) were observed in significant yield. The rotational distributions in both vibrational levels are essentially the same, and are characteristic of a Boltzmann distribution at a temperature close to that of the liquid surface. This contrasts with the distributions in the corresponding homogeneous gas-phase reactions. We propose a preliminary interpretation in terms of a dominant trapping-desorption mechanism, in which the OD molecules are retained at the surface sufficiently long to cause rotational equilibration but not complete vibrational relaxation. The significant yield of vibrationally excited OD also suggests that the surface is not composed entirely of -CD3 endgroups, but that secondary and/or tertiary units along the backbone are exposed.

  17. Excitons into one-axis crystals of zinc phosphide (Zn3P2

    Directory of Open Access Journals (Sweden)

    D.M. Stepanchikov

    2009-01-01

    Full Text Available Theoretical study of excitons spectra is offered in this report as for Zn3P2 crystals. Spectra are got in the zero approach of the theory of perturbations with consideration of both the anisotropy of the dispersion law and the selection rules. The existence of two exciton series was found, which corresponds to two valence bands (hh, lh and the conductivity band (c. It is noteworthy that anisotropy of the dispersion law plus the existence of crystalline packets (layers normal to the main optical axis, both will permit the consideration of two-dimensional excitons too. The high temperature displaying of these 2D-exciton effects is not eliminated even into bulk crystals. The calculated values of the binding energies as well as the oscillator's strength for the optical transitions are given for a volume (3D and for two-dimensional (2D excitons. The model of energy exciton transitions and four-level scheme of stimulated exciton radiation for receiving laser effect are offered.

  18. Ba 4/ital d/ core-level spectroscopy in the YBa/sub 2/Cu/sub 3/O/sub 6. 9/ high-/ital T//sub /ital c// superconductor: Existence of a surface-shifted component

    Energy Technology Data Exchange (ETDEWEB)

    Liu, R.; Olson, C. G.; Yang, A.; Gu, C.; Lynch, D. W.; Arko, A. J.; List, R. S.; Bartlett, R. J.; Veal, B. W.; Liu, J. Z.; and others

    1989-08-01

    Two sets of spin-orbit split Ba 4/ital d/ core-level photoemission peaks were observed in a crystal of YBa/sub 2/Cu/sub 3/O/sub 6.9/. From constant final-state measurements taken as a function of kinetic energy, the low-binding-energy doublet is identified as a surface component. Possible origins of the surface shift are discussed.

  19. Isotope shifts of the (3s3p) 3P0,1,2-(3s4s) 3S1 Mg I transitions

    DEFF Research Database (Denmark)

    He, Ming; Therkildsen, Kasper T.; Jensen, Brian B.;

    2009-01-01

    We report measurements of the isotope shifts of the (3s3p)P30,1,2-(3s4s)S31 Mg I transitions for the stable isotopes M24g (I=0) , M25g (I=5/2) , and M26g (I=0) . Furthermore, the M25g S31 hyperfine coefficient A(S31)=(-321.6±1.5)MHz is extracted and found to be in excellent agreement with state...

  20. The Application of 3P+MYSQL Technology of Dynamic Website Design%3P+MYSQL技术在动态网站设计中的运用

    Institute of Scientific and Technical Information of China (English)

    周悦; 朱小社

    2004-01-01

    在Windows2000 Server平台下分别利用ASP、PHP、JSP这三个动态网站技术,使用可以跨Windows和Linux平台的MYSQL数据库,采用三种连接数据库的方法,在三种不同特点的WEB服务器环境下,给出了动态网站设计中3P+MYSQL技术的运用.

  1. Study on core area strength training of college high level hoopsters%普通高校篮球运动员核心区力量训练的研究

    Institute of Scientific and Technical Information of China (English)

    张淑兰

    2011-01-01

    Using physiology, sports training, anatomy methods combined with basketball sports characteristics, the core area strength training of college hoopsters is studied. The action, signification and basic method of core area strength training are discussed, core area strength training process can improve the level of all function in athletes' body, and it benefits to athletics ability. It is references to coach in core area strength training arrangement.%运用生理学、运动训练学、解剖学等方法,结合篮球运动项目的专项特点,对高校高水平篮球运动员的核心区力量训练进行研究,并提出核心区力量训练在篮球专项体能训练中的作用、意义以及核心区力量训练的基本方法.通过核心区力量训练,可以有效提高运动员身体各项机能水平,对提高竞技能力有一定的促进作用,为教练员安排核心区力量训练提供相关的理论参考.

  2. Surface and interface properties of Zn3P2 solar cells

    Science.gov (United States)

    Kazmerski, L. L.; Ireland, P. J.; Catalano, A.

    1981-03-01

    The compositional properties of Zn3P2 thin films are studied using surface analysis techniques. Relative sensitivity factors of S(Zn sub LMM) = 0.29 and S(P sub LMM) = 0.34 are determined for this semiconductor with the aid of Auger electron spectroscopy standard spectra. The interfacial properties of the Mg/Zn3P2 thin-film solar cell are studied using Auger electron spectroscopy depth-compositional profiling in conjunction with complementary secondary ion mass spectroscopy and X-ray photoelectron spectroscopy data. Evidence is adduced for the formation of Mg3P2 at the metal-semiconductor interface. It is noted that this compound has a bandgap near that of Zn3P2. Attention is given to the possible formation of a heterojunction as a result of the interfacial reaction.

  3. Determination of diffusion, reflection and deexcitation coefficients of metastable excited Ne(3P2) atom

    Science.gov (United States)

    Suzuki, S.; Itoh, H.

    2016-05-01

    The diffusion coefficient of the metastable excited Ne(3P2) atom in neon, the reflection coefficient of Ne(3P2) at the surface of an electrode and the rate coefficient of Ne(3P2) for collisional quenching by Ne(1S0) were determined from the gas pressure dependence of the effective lifetime of Ne(3P2). The effective lifetime of Ne(3P2) was measured from the transient current after turning off the Ultraviolet (UV) light in a Townsend discharge. The observed transient current waveform was analysed by solving the diffusion equation for the metastable excited Ne(3P2) atom using the third kind of boundary condition. The rate coefficient of Ne(3P2) for collisional quenching by Ne(1S0) and the reflection coefficient were determined by a nonspectroscopic method for the first time in neon to the best of our knowledge and were (3.2  ±  0.4)  ×  10-16 cm3 s-1 and 0.10  ±  0.04, respectively. The obtained diffusion coefficient at 1 Torr was 177  ±  17 cm2 s-1, which is consistent with the value reported by Dixon and Grant. Moreover, the present results are compared with the results of Phelps and were found to be in good agreement. We also discuss the deexcitation rate of Ne(3P2) at pressures of up to 60 Torr in comparison with previously reported values.

  4. Misregulation of Scm3p/HJURP Causes Chromosome Instability in Saccharomyces cerevisiae and Human Cells

    OpenAIRE

    Prashant K. Mishra; Wei-Chun Au; John S. Choy; P Henning Kuich; Baker, Richard E.; Foltz, Daniel R.; Basrai, Munira A.

    2011-01-01

    The kinetochore (centromeric DNA and associated proteins) is a key determinant for high fidelity chromosome transmission. Evolutionarily conserved Scm3p is an essential component of centromeric chromatin and is required for assembly and function of kinetochores in humans, fission yeast, and budding yeast. Overexpression of HJURP, the mammalian homolog of budding yeast Scm3p, has been observed in lung and breast cancers and is associated with poor prognosis; however, the physiological relevanc...

  5. A new form of Ca{sub 3}P{sub 2} with a ring of Dirac nodes

    Energy Technology Data Exchange (ETDEWEB)

    Xie, Lilia S.; Schoop, Leslie M.; Seibel, Elizabeth M.; Gibson, Quinn D.; Xie, Weiwei; Cava, Robert J., E-mail: rcava@princeton.edu [Department of Chemistry, Princeton University, Princeton, New Jersey 08544 (United States)

    2015-08-01

    We report the synthesis and crystal structure of a new high-temperature form of Ca{sub 3}P{sub 2}. The crystal structure was determined through Rietveld refinements of synchrotron powder x-ray diffraction data. This form of Ca{sub 3}P{sub 2} has a crystal structure of the hexagonal Mn{sub 5}Si{sub 3} type, with a Ca ion deficiency compared to the ideal 5:3 stoichiometry. This yields a stable, charge-balanced compound of Ca{sup 2+} and P{sup 3−}. We also report the observation of a secondary hydride phase, Ca{sub 5}P{sub 3}H, which again is a charge-balanced compound. The calculated band structure of Ca{sub 3}P{sub 2} indicates that it is a three-dimensional Dirac semimetal with a highly unusual ring of Dirac nodes at the Fermi level. The Dirac states are protected against gap opening by a mirror plane in a manner analogous to what is seen for graphene.

  6. Hepatitis B core-related antigen (HBcrAg) levels in the natural history of hepatitis B virus infection in a large European cohort predominantly infected with genotypes A and D.

    Science.gov (United States)

    Maasoumy, B; Wiegand, S B; Jaroszewicz, J; Bremer, B; Lehmann, P; Deterding, K; Taranta, A; Manns, M P; Wedemeyer, H; Glebe, D; Cornberg, M

    2015-06-01

    Hepatitis B core-related antigen (HBcrAg) has been suggested as an additional marker of hepatitis B virus (HBV) infection. HBcrAg combines the antigenic reactivity resulting from denatured hepatitis B e antigen (HBeAg), HBV core antigen and an artificial core-related protein (p22cr). In Asian patients, high levels of HBcrAg have been suggested to be an independent risk factor for hepatocellular carcinoma, while low levels could guide safe cessation of treatment with nucleos(t)ide analogues. We here studied HBcrAg levels in different phases of HBV infection in a large European cohort predominantly infected with genotypes A and D: HBeAg-positive immune tolerance (n = 30), HBeAg-positive immune clearance (IC) (n = 60), HBeAg-negative hepatitis (ENH) (n = 50), HBeAg-negative inactive/quiescent carrier phase (c) (n = 109) and acute hepatitis B (n = 8). Median HBcrAg levels were high in the immune tolerance and immune clearance phases (8.41 and 8.11 log U/mL, respectively), lower in ENH subjects (4.82 log U/mL) but only 2.00 log U/mL in ENQ subjects. Correlation between HBcrAg and HBV DNA varied among the different phases of HBV infection, while HBcrAg moderately correlated with hepatitis B surface antigen in all phases. ENQ patients had HBcrAg levels HBcrAg levels vary significantly during the different phases of HBV infection. HBcrAg may serve as valuable marker for virus replication and reflect the transcriptional activity of intrahepatic cccDNA. In HBeAg-negative patients, HBcrAg may help to distinguish between inactive carriers (ENQ) and those with active disease (ENH).

  7. Structure and Function of the PLAA/Ufd3-p97/Cdc48 Complex

    Energy Technology Data Exchange (ETDEWEB)

    Qiu, Liyan; Pashkova, Natasha; Walker, John R.; Winistorfer, Stanley; Allali-Hassani, Abdellah; Akutsu, Masato; Piper, Robert; Dhe-Paganon, Sirano (Iowa); (Toronto)

    2010-02-11

    PLAA (ortholog of yeast Doa1/Ufd3, also know as human PLAP or phospholipase A2-activating protein) has been implicated in a variety of disparate biological processes that involve the ubiquitin system. It is linked to the maintenance of ubiquitin levels, but the mechanism by which it accomplishes this is unclear. The C-terminal PUL (PLAP, Ufd3p, and Lub1p) domain of PLAA binds p97, an AAA ATPase, which among other functions helps transfer ubiquitinated proteins to the proteasome for degradation. In yeast, loss of Doa1 is suppressed by altering p97/Cdc48 function indicating that physical interaction between PLAA and p97 is functionally important. Although the overall regions of interaction between these proteins are known, the structural basis has been unavailable. We solved the high resolution crystal structure of the p97-PLAA complex showing that the PUL domain forms a 6-mer Armadillo-containing domain. Its N-terminal extension folds back onto the inner curvature forming a deep ridge that is positively charged with residues that are phylogenetically conserved. The C terminus of p97 binds in this ridge, where the side chain of p97-Tyr805, implicated in phosphorylation-dependent regulation, is buried. Expressed in doa1{Delta} null cells, point mutants of the yeast ortholog Doa1 that disrupt this interaction display slightly reduced ubiquitin levels, but unlike doa1{Delta} null cells, showed only some of the growth phenotypes. These data suggest that the p97-PLAA interaction is important for a subset of PLAA-dependent biological processes and provides a framework to better understand the role of these complex molecules in the ubiquitin system.

  8. Relativistic frozen core potential scheme with relaxation of core electrons

    Science.gov (United States)

    Nakajima, Yuya; Seino, Junji; Hayami, Masao; Nakai, Hiromi

    2016-10-01

    This letter proposes a relaxation scheme for core electrons based on the frozen core potential method at the infinite-order Douglas-Kroll-Hess level, called FCP-CR. The core electrons are self-consistently relaxed using frozen molecular valence potentials after the valence SCF calculation is performed. The efficiency of FCP-CR is confirmed by calculations of gold clusters. Furthermore, FCP-CR reproduces the results of the all-electron method for the energies of coinage metal dimers and the core ionization energies and core level shifts of vinyl acetate and three tungsten complexes at the Hartree-Fock and/or symmetry-adapted cluster configuration interaction levels.

  9. Cu3P/RGO Nanocomposite as a New Anode for Lithium-Ion Batteries

    Science.gov (United States)

    Liu, Shuling; He, Xiaodong; Zhu, Jianping; Xu, Liqiang; Tong, Jianbo

    2016-10-01

    Cu3P/reduced graphene oxide (Cu3P/RGO) nanocomposite was successfully synthesized by a facile one-pot method as an advanced anode material for high-performance lithium-ion batteries. Cu3P nanostructures with a polyhedral shape with the mean diameter (80–100 nm) were homogeneously anchored on the surface of RGO. The flexible RGO sheets acted as elastic buffering layer which not only reduced the volume change, but also prevented the aggregation of Cu3P nanostructures, the cracking and crumbing of electrodes. On the other hand, the presence of Cu3P nanostructures could also avoid the agglomeration of RGO sheets and retain their highly active surface area. Therefore, as an advanced anode material for high-performance lithium-ion batteries, the as-prepared Cu3P/RGO exhibited high capacity of 756.15 mAhg‑1 at the current density 500 mAg‑1 after 80 cycles, superior cyclic stability and good rate capability.

  10. Establishment and application of a method for the detection of the core fucosylated IgG level%IgG核心岩藻糖基化水平检测方法的建立及应用

    Institute of Scientific and Technical Information of China (English)

    衣常红; 王坤; 李慧; 高春芳

    2011-01-01

    To build up a method for the detection of the core fucosylated IgG level and to figure out the relationship between the core fucosylated level and aberrance of high IgG concentration in HBV-related liver cirrhosis, the core fucosylated serum protein was purified with specific core fucose binding lectin LCA agglutinin affinity column.and then the levels of the core fucosylated total protein (TP) and IgG of patients with HBV-related liver cirrhosis were calculated and analyzed.It was found that the level of LCA binding IgG was higher in patients with HBV-related liver cirrhosis in comparison with that of controls and chronic hepatitis B patients (P<0.05).This seemed to be a valuable alternative for the identification of HBV-related liver cirrhosis.The increased level of core fucosylated IgG may be attribute partially or totally to the attenuated function of IgG.This method of testing the core fucosylated IgG level is promising to be a noninvasive serum marker for the diagnosis and may be a potential therapeutic target for the immune regulation in liver cirrhosis.%为建立IgG核心岩藻糖基化水平检测方法,研究血清IgG核心岩藻糖基化水平与慢性乙肝(CHB)、肝硬化(LC)中IgG异常增高的关系.应用特异性结合核心岩藻糖的LCA凝集素亲和柱分离纯化血清中具有的核心岩藻糖基化蛋白,并对CHB、LC患者的血清总蛋白(TP)和免疫球蛋白G(IgG)的核心岩藻糖基化水平进行检测和对比分析.结果显示:LC患者IgG及其核心岩藻精基化水平较正常组和CHB组均升高且存在统计学差异(P<0.05),并对LC具有诊断提示作用.表明:IgG核心岩藻糖糖基化水平的增高可能是LC中IgG异常增高和IgG功能部分丧失的原因之一,有望成为一个新的无创性肝纤维化监测、诊断指标,也为可能的免疫调控干预提供了潜在的治疗靶点.

  11. Performance and advantages of a soft-core based parallel architecture for energy peak detection in the calorimeter Level 0 trigger for the NA62 experiment at CERN

    Science.gov (United States)

    Ammendola, R.; Barbanera, M.; Bizzarri, M.; Bonaiuto, V.; Ceccucci, A.; Checcucci, B.; De Simone, N.; Fantechi, R.; Federici, L.; Fucci, A.; Lupi, M.; Paoluzzi, G.; Papi, A.; Piccini, M.; Ryjov, V.; Salamon, A.; Salina, G.; Sargeni, F.; Venditti, S.

    2017-03-01

    The NA62 experiment at CERN SPS has started its data-taking. Its aim is to measure the branching ratio of the ultra-rare decay K+ → π+ν ν̅ . In this context, rejecting the background is a crucial topic. One of the main background to the measurement is represented by the K+ → π+π0 decay. In the 1-8.5 mrad decay region this background is rejected by the calorimetric trigger processor (Cal-L0). In this work we present the performance of a soft-core based parallel architecture built on FPGAs for the energy peak reconstruction as an alternative to an implementation completely founded on VHDL language.

  12. Microduplications of 3p26.3p26.2 containing CRBN gene in patients with intellectual disability and behavior abnormalities.

    Science.gov (United States)

    Papuc, Sorina M; Hackmann, Karl; Andrieux, Joris; Vincent-Delorme, Catherine; Budişteanu, Magdalena; Arghir, Aurora; Schrock, Evelin; Ţuţulan-Cuniţă, Andreea C; Di Donato, Nataliya

    2015-05-01

    We report on the clinical data and molecular cytogenetic findings in three unrelated patients presenting with intellectual disability and behavior abnormalities. An overlapping microduplication involving 3p26.2-26.3 was identified in these patients. All three aberrations were confirmed and proven to be parentally inherited. The sizes of the duplications were different, with a common minimal region of 423,754 bp containing two genes - TRNT1 and CRBN. Here, we hypothesize that the copy number gain of CRBN gene might be responsible for developmental delay/intellectual disability.

  13. miR-509-3p is clinically significant and strongly attenuates cellular migration and multi-cellular spheroids in ovarian cancer.

    Science.gov (United States)

    Pan, Yinghong; Robertson, Gordon; Pedersen, Lykke; Lim, Emilia; Hernandez-Herrera, Anadulce; Rowat, Amy C; Patil, Sagar L; Chan, Clara K; Wen, Yunfei; Zhang, Xinna; Basu-Roy, Upal; Mansukhani, Alka; Chu, Andy; Sipahimalani, Payal; Bowlby, Reanne; Brooks, Denise; Thiessen, Nina; Coarfa, Cristian; Ma, Yussanne; Moore, Richard A; Schein, Jacquie E; Mungall, Andrew J; Liu, Jinsong; Pecot, Chad V; Sood, Anil K; Jones, Steven J M; Marra, Marco A; Gunaratne, Preethi H

    2016-05-03

    Ovarian cancer presents as an aggressive, advanced stage cancer with widespread metastases that depend primarily on multicellular spheroids in the peritoneal fluid. To identify new druggable pathways related to metastatic progression and spheroid formation, we integrated microRNA and mRNA sequencing data from 293 tumors from The Cancer Genome Atlas (TCGA) ovarian cancer cohort. We identified miR-509-3p as a clinically significant microRNA that is more abundant in patients with favorable survival in both the TCGA cohort (P = 2.3E-3), and, by in situ hybridization (ISH), in an independent cohort of 157 tumors (P < 1.0E-3). We found that miR-509-3p attenuated migration and disrupted multi-cellular spheroids in HEYA8, OVCAR8, SKOV3, OVCAR3, OVCAR4 and OVCAR5 cell lines. Consistent with disrupted spheroid formation, in TCGA data miR-509-3p's most strongly anti-correlated predicted targets were enriched in components of the extracellular matrix (ECM). We validated the Hippo pathway effector YAP1 as a direct miR-509-3p target. We showed that siRNA to YAP1 replicated 90% of miR-509-3p-mediated migration attenuation in OVCAR8, which contained high levels of YAP1 protein, but not in the other cell lines, in which levels of this protein were moderate to low. Our data suggest that the miR-509-3p/YAP1 axis may be a new druggable target in cancers with high YAP1, and we propose that therapeutically targeting the miR-509-3p/YAP1/ECM axis may disrupt early steps in multi-cellular spheroid formation, and so inhibit metastasis in epithelial ovarian cancer and potentially in other cancers.

  14. Doubly excited 2s2p 1,3p1 resonances in photoionization of helium

    Institute of Scientific and Technical Information of China (English)

    Wan Jian-Jie; Dong Chen-Zhong

    2009-01-01

    The multi-configuration Dirac-Fock (MCDF) method is implemented to study doubly excited 2s2p 1,3P1 resonances of the helium atom and the interference between photoionization and photoexcitation autoionization processes.In order to reproduce the total photoionization sprectra,the excited energies from the ground ls2 1 S0 state to the doubly excited 2s2p 1'3P1 states and the relevant Auger decay rates and widths are calculated in detail. Furthermore,the interference profile determined by the so-called Fano parameters q and p2 is also reproduced. Good agreement is found between the present results and other available theoretical and experimental results. This indeed shows a promising way to investigate the Fano resonances in photoionization of atoms within the MCDF scheme,although there are some discrepancies in the present calculations of the 2s2p 3P1 state.

  15. Experimental and theoretical study of 3p photoionization and subsequent Auger decay in atomic chromium

    Science.gov (United States)

    Keskinen, J.; Huttula, S.-M.; Mäkinen, A.; Patanen, M.; Huttula, M.

    2015-12-01

    3p photoionization and subsequent low kinetic energy Coster-Kronig and super Coster-Kronig Auger decay have been studied in atomic chromium. The binding energies, line widths, and relative intensities for the transitions seen in the synchrotron radiation excited 3p photoelectron spectrum are determined. The high resolution M2,3 M4,5 M4,5 and M2,3 M4,5 N1 Auger electron spectra following the electron impact excited 3p ionization are presented and the kinetic energies, relative intensities, and identifications are given for the main lines. The experimental findings are compared with the theoretical predictions obtained from Hartree-Fock and multiconfiguration Dirac-Fock approaches.

  16. MicroRNA-450a-3p represses cell proliferation and regulates embryo development by regulating Bub1 expression in mouse.

    Directory of Open Access Journals (Sweden)

    Min Luo

    Full Text Available Bub1 is a critical component of the spindle assembly checkpoint (SAC and closely linked to cell proliferation and differentiation. We previously found that spontaneous abortion embryos contained a low level of Bub1 protein but normal mRNA level, while the knockdown of Bub1 leads to abnormal numerical chromosomes in embryonic cells. Here, we investigated the mechanism through which governs the post-transcriptional regulation of Bub1 protein expression level. We first conducted bioinformatics analysis and identified eight putative miRNAs that may target Bub1. Luciferase reporter assay confirmed that miR-450a-3p can directly regulate Bub1 by binding to the 3'-untranslated region of Bub1 mRNA. We found that the overexpression of miR-450a-3p in mouse embryonic fibroblast (MEF cells down-regulated Bub1 protein level, repressed cell proliferation, increased apoptosis and restricted most cells in G1 phase of the cell cycle. Furthermore, when the fertilized eggs were microinjected with miR-450a-3p mimics, the cleavage of zygotes was effectively suppressed. Our results strongly suggest that an abnormally decreased Bub1 level regulated by miRNAs may be implicated in the pathogenesis of spontaneous miscarriage. Therefore, the blockade of miR-450a-3p may be explored as a novel therapeutic strategy for preventing spontaneous miscarriages.

  17. Domain architectures of the Scm3p protein provide insights into centromere function and evolution

    OpenAIRE

    Aravind, L.; Lakshminarayan, M. Iyer; Wu, Carl

    2007-01-01

    Recently, Scm3p has been shown to be a nonhistone component of centromeric chromatin that binds stoichiometrically to CenH3–H4 histones, and to be required for the assembly of kinetochores in S. cerevisiae. Scm3p is conserved across fungi, and displays a remarkable variation in protein size, ranging from ~200 amino acids in Saccharomyces cerevisiae to ~1300 amino acids in Neurospora crassa. This is primarily due a variable C-terminal segment that is linked to a conserved N-terminal, CenH3-int...

  18. Autism spectrum disorder, Klinefelter syndrome, and chromosome 3p21.31 duplication: a case report.

    Science.gov (United States)

    Stuart, Scott W; King, Casey H; Pai, G Shashidar

    2007-12-18

    Autism spectrum disorders are heterogeneous in nature with idiopathic and genetic origins. We present a 7-year-old boy with a long history of multiple behavioral concerns, poor school performance, repetitive/compulsive tendencies, poor social skills, and language delays. A multidisciplinary evaluation concluded that the patient met full criteria for autism. A genetic evaluation demonstrated Klinefelter syndrome 47, XXY karyotype with concurrent duplication of 3p21.31 by microarray analysis. Maternal genetic analysis demonstrated the same 3p21.31 duplication. The potential implication with regard to autism spectrum disorders has not been previously discussed in the literature.

  19. Promoting marketing management level to the development assistance for the core of grid enterprises%提升营销管理水平的探讨

    Institute of Scientific and Technical Information of China (English)

    罗伦国

    2011-01-01

    New power marketing method to meet the market needs should be established, taking natural resources as premise, based on demand forecasting management, for the purpose of quality services, centered in meet user needs and guide the consumer. Grid enterprises is always ready to offer quality and cheap cleaner energy, is adapted to commercializes operation and legalized management, have layered efficient operating and full - featured, which is the fierce urgency of its core business--the marketing management work.%建立适应市场需要的新型电力营销理念,以可持续发展为前提,以需求预测管理为基础,以用户需求为导向,以优质服务为宗旨,以满足用户需求、引导用户消费为中心,随时随地为用户提供质优、价廉的清洁能源,适应商业化运营、法制化管理,分层高效运作,功能齐备,是电网企业核心业务---营销管理工作的当前之急。

  20. Human Cytomegalovirus miR-UL112-3p Targets TLR2 and Modulates the TLR2/IRAK1/NFκB Signaling Pathway.

    Directory of Open Access Journals (Sweden)

    Igor Landais

    2015-05-01

    Full Text Available Human Cytomegalovirus (HCMV encodes multiple microRNAs (miRNAs whose functions are just beginning to be uncovered. Using in silico approaches, we identified the Toll-Like Receptor (TLR innate immunity pathway as a possible target of HCMV miRNAs. Luciferase reporter assay screens further identified TLR2 as a target of HCMV miR-UL112-3p. TLR2 plays a major role in innate immune response by detecting both bacterial and viral ligands, including HCMV envelope proteins gB and gH. TLR2 activates a variety of signal transduction routes including the NFκB pathway. Furthermore, TLR2 plays an important role in controlling CMV infection both in humans and in mice. Immunoblot analysis of cells transfected with a miR-UL112-3p mimic revealed that endogenous TLR2 is down-regulated by miR-UL112-3p with similar efficiency as a TLR2-targeting siRNA (siTLR2. We next found that TLR2 protein level decreases at late times during HCMV infection and correlates with miR-UL112-3p accumulation in fibroblasts and monocytic THP1 cells. Confirming direct miR-UL112-3p targeting, down-regulation of endogenous TLR2 was not observed in cells infected with HCMV mutants deficient in miR-UL112-3p expression, but transfection of miR-UL112-3p in these cells restored TLR2 down-regulation. Using a NFκB reporter cell line, we found that miR-UL112-3p transfection significantly inhibited NFκB-dependent luciferase activity with similar efficiency as siTLR2. Consistent with this observation, miR-UL112-3p transfection significantly reduced the expression of multiple cytokines (IL-1β, IL-6 and IL-8 upon stimulation with a TLR2 agonist. Finally, miR-UL112-3p transfection significantly inhibited the TLR2-induced post-translational activation of IRAK1, a kinase located in the upstream section of the TLR2/NFκB signaling axis. To our knowledge, this is the first identified mechanism of TLR2 modulation by HCMV and is the first report of functional targeting of TLR2 by a viral miRNA. These

  1. The infinite order point on Y~2=X~3-p~3

    Institute of Scientific and Technical Information of China (English)

    张绍伟

    1995-01-01

    The parameterization of the elliptic curve Y2=X2+1 is given by using the modular forms of X(12) Then using class field theory over imaginary quadratic field and Shimura reciprocity, an infinite order point on the curve y2- = x3-p3 is constructed, for prime p=7 (mod 24).

  2. miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation

    DEFF Research Database (Denmark)

    Qiu, Weimin; Kassem, Moustapha

    2014-01-01

    Wnt signaling determines human stromal (mesenchymal) stem cell (hMSC) differentiation fate into the osteoblast or adipocyte lineage. microRNAs (miRNAs) are small RNA molecules of 21-25 nucleotides that regulate many aspects of osteoblast biology. Thus, we examined miRNAs regulated by Wnt signaling...... in hMSC. We identified miRNA (miR)-141-3p as a Wnt target which in turn inhibited Wnt signaling. Moreover, miR-141-3p inhibited hMSC proliferation by arresting cells at the G1 phase of the cell cycle. miR-141-3p inhibited osteoblast differentiation of hMSC as evidenced by reduced alkaline phosphatase...... activity, gene expression and in vitro mineralized matrix formation. Bioinformatic studies, Western blot analysis and 3'UTR reporter assay demonstrated that cell division cycle 25A (CDC25A) is a direct target of miR-141-3p. siRNA-mediated knock-down of CDC25A inhibited hMSC proliferation and osteoblast...

  3. Construction of a consistent YAC contig for human chromosome region 3p14.1

    NARCIS (Netherlands)

    Bardenheuer, W; Michaelis, S; Lux, A; Vieten, L; Brocker, F; Julicher, K; Willers, C; Siebert, R; Smith, DI; vanderHout, AH; Buys, C; Schutte, J; Opalka, B

    1996-01-01

    Chromosomal deletions and translocations of human chromosome region 3p14 are observed in various human malignancies and suggest the existence of a tumor suppressor gene locus within this region. Tumors most frequently affected by these aberrations are small-cell lung cancer and renal-cell carcinoma.

  4. Baryonic {sup 3}P{sub 2} superfluidity under charged-pion condensation with {delta} isobar

    Energy Technology Data Exchange (ETDEWEB)

    Takatsuka, T.; Tamagaki, R. [Iwate Univ., Morioka, Iwate (Japan)

    1999-08-01

    We study the baryonic {sup 3}P{sup 2} superfluidity under charged-pion condensation with isobar ({delta}) degrees of freedom. After a remark on motivations of the present study, the outline of theoretical framework is briefly described, typical results of the superfluid critical temperature are shown, and the possibility of coexistence of the superfluid with charged-pion condensation is discussed. (author)

  5. Using P3P in a web services-based context-aware application platform

    NARCIS (Netherlands)

    Zuidweg, M.; Goncalves Filho, J.; Sinderen, van M.J.; Halasz, E.; Lukovszki, C.; Marosits, T.

    2003-01-01

    This paper describes a proposal for a privacy control architecture to be applied in the WASP project. The WASP project aims to develop a context-aware service platform on top of 3G networks, using web services technology. The proposed privacy control architecture is based on the P3P privacy policy d

  6. Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis.

    Science.gov (United States)

    Budak, Ferah; Bal, Salih Haldun; Tezcan, Gulcin; Akalın, Halis; Goral, Guher; Oral, Haluk Barbaros

    2016-01-01

    Brucellosis is a zoonotic disease that is still endemic in developing countries. Despite early diagnosis and treatment of patients, chronic infections are seen in 10-30% of patients. In this study, we aimed to investigate the immunological factors that play roles in the transition of brucellosis from acute infection into chronic infection. Here, more than 2000 miRNAs were screened in peripheral blood mononuclear cells (PBMCs) of patients with acute or chronic brucellosis and healthy controls by using miRNA array, and the results of the miRNA array were validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Four miRNAs were expressed in the chronic group but were not expressed in acute and control groups. Among these miRNAs, the expression level of miR-1238-3p was increased while miR-494, miR-6069, and miR-139-3p were decreased (p 2). These miRNAs have the potential to be markers for chronic cases. The differentially expressed miRNAs and their predicted target genes involved in endocytosis, regulation of actin cytoskeleton, MAPK signaling pathway, and cytokine-cytokine receptor interaction and its chemokine signaling pathway indicate their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human PBMC to clarify the mechanism of inveteracy in brucellosis.

  7. Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis

    Science.gov (United States)

    Budak, Ferah; Bal, Salih Haldun; Tezcan, Gulcin; Akalın, Halis; Goral, Guher

    2016-01-01

    Brucellosis is a zoonotic disease that is still endemic in developing countries. Despite early diagnosis and treatment of patients, chronic infections are seen in 10–30% of patients. In this study, we aimed to investigate the immunological factors that play roles in the transition of brucellosis from acute infection into chronic infection. Here, more than 2000 miRNAs were screened in peripheral blood mononuclear cells (PBMCs) of patients with acute or chronic brucellosis and healthy controls by using miRNA array, and the results of the miRNA array were validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Four miRNAs were expressed in the chronic group but were not expressed in acute and control groups. Among these miRNAs, the expression level of miR-1238-3p was increased while miR-494, miR-6069, and miR-139-3p were decreased (p 2). These miRNAs have the potential to be markers for chronic cases. The differentially expressed miRNAs and their predicted target genes involved in endocytosis, regulation of actin cytoskeleton, MAPK signaling pathway, and cytokine-cytokine receptor interaction and its chemokine signaling pathway indicate their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human PBMC to clarify the mechanism of inveteracy in brucellosis. PMID:27722176

  8. Toward a model for assessing level of personality functioning in DSM-5, part II: empirical articulation of a core dimension of personality pathology

    NARCIS (Netherlands)

    L.C. Morey; H. Berghuis; D.S. Bender; R. Verheul; R.F. Krueger; A.E. Skodol

    2011-01-01

    The extensive comorbidity among Diagnostic and Statistical Manual of Mental Disorders (4th ed. [DSM-IV]; American Psychiatric Association, 1994) personality disorders might be compelling evidence of essential commonalities among these disorders reflective of a general level of personality functionin

  9. Reactions of N+ (3P) ions with H2 and HD molecules at low temperatures

    Science.gov (United States)

    Grozdanov, Tasko P.; McCarroll, Ronald; Roueff, Evelyne

    2016-05-01

    formation of ND+. The calculated value is consistent with the available experimental data. Conclusions: The present results allow for the determination of reaction rate coefficients for any given distribution of specific fine structure and rotational state populations of the reactants. In interstellar conditions, where N+ is in its 3P0 state and para- and ortho-H2 respectively in J = 0 and J = 1. Our results enable a study of the influence of the ortho/para evolution of molecular hydrogen on the formation of nitrogen compounds.

  10. Mitochondria-related miR-141-3p contributes to mitochondrial dysfunction in HFD-induced obesity by inhibiting PTEN.

    Science.gov (United States)

    Ji, Juan; Qin, Yufeng; Ren, Jing; Lu, Chuncheng; Wang, Rong; Dai, Xiuliang; Zhou, Ran; Huang, Zhenyao; Xu, Miaofei; Chen, Minjian; Wu, Wei; Song, Ling; Shen, Hongbing; Hu, Zhibin; Miao, Dengshun; Xia, Yankai; Wang, Xinru

    2015-11-09

    Mitochondria-related microRNAs (miRNAs) have recently emerged as key regulators of cell metabolism and can modulate mitochondrial fusion and division. In order to investigate the roles of mitochondria-related miRNAs played in obesity, we conducted comprehensive molecular analysis in vitro and in vivo. Based on high-fat-diet (HFD) induced obese mice, we found that hepatic mitochondrial function was markedly altered. Subsequently, we evaluated the expression levels of selected mitochondria-related miRNAs and found that miR-141-3p was up-regulated strikingly in HFD mice. To further verify the role of miR-141-3p in obesity, we carried out gain-and-loss-of-function study in human HepG2 cells. We found that miR-141-3p could modulate ATP production and induce oxidative stress. Through luciferase report gene assay, we identified that phosphatase and tensin homolog (PTEN) was a target of miR-141-3p. Inhibiting PTEN could alter the mitochondrial function, too. Our study suggested that mitochondria-related miR-141-3p induced mitochondrial dysfunction by inhibiting PTEN.

  11. Experience of training core competencies for junior nurses ( N1-level )in intensive care units%ICU低年资护士N1级核心能力培训体会

    Institute of Scientific and Technical Information of China (English)

    林育群; 许实燕; 郑杏桔

    2011-01-01

    目的:探讨适合ICU低年资护士N1级核心能力培训的方法.方法:评估低年资护士核心能力,确定岗位职级,通过集中授课、现场观摩、示范、自学、病例讨论、查房等,进行核心能力培训,培训分为3个阶段进行,全部完成时间2~3年,评价内容包括专科理论和专业知识、技能、案例项目积累完成情况及分析能力、实际工作的应对能力.观察指标为N1级护士培训前后核心能力各项的分值及ICU各项护理质量指标的变化.结果:培训后低年资护士核心能力、ICU各项护理质量指标明显提高.结论:专业核心能力N1级培训有利于提高低年资护士核心能力及保证护理质量.%Objective: To find an effective method of training core competencies for junior nurses ( N1-level )in intensive care units. Methods: According to the nurses in different class of position, We trained the nurses by the centralism teach ing, live visit, demonstration, self-study, case discussions, patient rounds, etc. the training was divided into three stages and completed in 2-3 years. the evaluation content including professional knowledge and technology, completion of the project accumulating case, analysis and the Capacity to respond to practical work. the scores of the nurses' core competen cies before and after training and the changes of nursing quality indicators of ICU were analyzed. Results: Core competen cies and nursing quality indicators of the junior nurses in ICU were improved significantly after the training. Conclusion: The core competence training help to improve core competence of the junior nurses and ensure quality of medical care.

  12. 护理本科生核心能力相关影响因素研究%The level and influencing factors of baccalaureate nursing students' core competency

    Institute of Scientific and Technical Information of China (English)

    刘彦慧; 廖瑞雪

    2014-01-01

    目的 了解本科护理学生核心能力现状,探讨影响其核心能力的影响因素,为提高护生的核心能力水平,促进护理事业的可持续发展提供科学依据.方法 2013年5~6月,采用方便抽样的方法,抽取天津市三级甲等医院实习的本科护理实习生280名为调查对象,采用Li-Ling Hsu等编制的护生核心能力量表(CINS)进行调查,对影响护理本科生核心能力的相关因素进行分析.结果 本科护生核心能力水平为(5.379±0.744)分,其中,得分最高的是道德和责任能力维度(5.840±0.827)分,得分最低的是批判性思维能力维度(4.794±1.105)分.多元回归方程结果显示,曾认真完成作业及准备考试对护生核心能力影响最大,其次为毕业后最想服务的单位及性别.结论 本科护生的核心能力水平有待进一步的提高,护理教育者和管理者应该重视对护生核心能力的培养,针对影响本科护生核心能力的相关影响因素,制定提高护生核心能力的策略,提高本科护生的核心能力水平.%Objective To investigate the level and influencing factors of baccalaureate nursing students' core competency,and supply scientific reference for sustainable development of nursing career.Methods From May to June 2013,totally 280 baccalaureate nursing students from a third-level hospital in Tianjin were tested by the Chinese version of CINS edited by Li-Ling Hsu.Results The score of nursing students' core competency was (5.379±0.744),and the highest score was the moral and responsibility ability dimension (5.840 ±0.827),the lowest score was the critical thinking ability dimension (4.794 ± 1.105).Multiple linear regression analysis showed that ever finishing homework and preparing for the exam carefully,deciding the service units after graduation and gender were the influencing factors of nursing students' core competency.Conclusions Nursing students' core competency needs to be further improved

  13. miR-144-3p, a tumor suppressive microRNA targeting ETS-1 in laryngeal squamous cell carcinoma.

    Science.gov (United States)

    Zhang, Si-Yi; Lu, Zhong-Ming; Lin, Ye-Feng; Chen, Liang-Si; Luo, Xiao-Ning; Song, Xin-Han; Chen, Shao-Hua; Wu, Yi-Long

    2016-03-08

    Regional lymph node metastasis and distant metastasis are critical in the prognosis of laryngeal squamous cell carcinoma (LSCC). This study investigated the roles of miR-144-3p and E26 transformation specific-1 (ETS-1) in the invasion and migration of LSCC cells. The effects of miR-144-3p and ETS-1 on FaDu and Hep2 cell growth, migration and invasion were determined. Suppression of ETS-1 by miR-144-3p was confirmed using luciferase assays; the effects of ETS-1 silencing were determined using a xenograft tumor model. The expression of ETS-1 was analyzed in 71 paraffin-embedded tissue biopsies and eight fresh frozen biopsies obtained from LSCC patients. miR-144-3p inhibited the growth, invasion and migration of FaDu and Hep2 cells in part through suppression of epithelial-mesenchymal transition as determined by increased E-cadherin and α-catenin and reduced fibronectin and vimentin expression. Additionally, ETS-1 is a molecular target of miR-144-3p, and silencing ETS-1 expression inhibited FaDu and Hep2 cell invasion and migration as well as reduced Hep2 xenograft tumor volume. In LSCC, the expression of ETS-1 is upregulated with disease progression, and higher ETS-1 expression, which was negatively associated with miR-144-3p levels, adversely corresponded with prognoses. Thus, upregulated ETS-1 levels may promote LSCC metastasis, resulting in poor patient prognosis.

  14. miR-509-3p is clinically significant and strongly attenuates cellular migration and multi-cellular spheroids in ovarian cancer

    Science.gov (United States)

    Pedersen, Lykke; Lim, Emilia; Hernandez-Herrera, Anadulce; Rowat, Amy C.; Patil, Sagar L.; Chan, Clara K.; Wen, Yunfei; Zhang, Xinna; Basu-Roy, Upal; Mansukhani, Alka; Chu, Andy; Sipahimalani, Payal; Bowlby, Reanne; Brooks, Denise; Thiessen, Nina; Coarfa, Cristian; Ma, Yussanne; Moore, Richard A.; Schein, Jacquie E.; Mungall, Andrew J.; Liu, Jinsong; Pecot, Chad V.; Sood, Anil K.; Jones, Steven J.M.; Marra, Marco A.; Gunaratne, Preethi H.

    2016-01-01

    Ovarian cancer presents as an aggressive, advanced stage cancer with widespread metastases that depend primarily on multicellular spheroids in the peritoneal fluid. To identify new druggable pathways related to metastatic progression and spheroid formation, we integrated microRNA and mRNA sequencing data from 293 tumors from The Cancer Genome Atlas (TCGA) ovarian cancer cohort. We identified miR-509-3p as a clinically significant microRNA that is more abundant in patients with favorable survival in both the TCGA cohort (P = 2.3E–3), and, by in situ hybridization (ISH), in an independent cohort of 157 tumors (P migration and disrupted multi-cellular spheroids in HEYA8, OVCAR8, SKOV3, OVCAR3, OVCAR4 and OVCAR5 cell lines. Consistent with disrupted spheroid formation, in TCGA data miR-509-3p's most strongly anti-correlated predicted targets were enriched in components of the extracellular matrix (ECM). We validated the Hippo pathway effector YAP1 as a direct miR-509-3p target. We showed that siRNA to YAP1 replicated 90% of miR-509-3p-mediated migration attenuation in OVCAR8, which contained high levels of YAP1 protein, but not in the other cell lines, in which levels of this protein were moderate to low. Our data suggest that the miR-509-3p/YAP1 axis may be a new druggable target in cancers with high YAP1, and we propose that therapeutically targeting the miR-509-3p/YAP1/ECM axis may disrupt early steps in multi-cellular spheroid formation, and so inhibit metastasis in epithelial ovarian cancer and potentially in other cancers. PMID:27036018

  15. Core Journal Lists: Classic Tool, New Relevance

    Science.gov (United States)

    Paynter, Robin A.; Jackson, Rose M.; Mullen, Laura Bowering

    2010-01-01

    Reviews the historical context of core journal lists, current uses in collection assessment, and existing methodologies for creating lists. Outlines two next generation core list projects developing new methodologies and integrating novel information/data sources to improve precision: a national-level core psychology list and the other a local…

  16. Lnc-ATB contributes to gastric cancer growth through a MiR-141-3p/TGFβ2 feedback loop.

    Science.gov (United States)

    Lei, Kecheng; Liang, Xin; Gao, Yuwei; Xu, Baixue; Xu, Yichun; Li, Yueqi; Tao, Yiwen; Shi, Weibin; Liu, Jianwen

    2017-03-11

    The long noncoding RNA (lncRNA) ATB is an important regulator in human tumors. Here, we aimed to investigate the potential molecular mechanisms of lnc-ATB in gastric cancer (GC) tumorigenesis. RT-qPCR analysis was used to detect lnc-ATB expression level in 20 pairs of gastric cancer tissues and adjacent normal gastric mucosa tissues (ANTs). Moreover, the biological role of lnc-ATB was determined in vitro. We found that lnc-ATB was significantly upregulated in GC tissues compared to lnc-ATB expression in ANTs. These high lnc-ATB expression levels predicted poor prognosis in GC patients. Low levels of lnc-ATB inhibited GC cell proliferation and cell cycle arrest in vitro. Lnc-ATB was found to directly bind miR-141-3p. Moreover, TGF-β actives lnc-ATB and TGF-β2 directly binds mir-141-3p. Finally, we demonstrated that lnc-ATB fulfilled its oncogenic roles in a ceRNA-mediated manner. Our study suggests that lnc-ATB promotes tumor progression by interacting with miR-141-3p and that Lnc-ATB may be a valuable prognostic predictor for GC. In conclusion, the positive feedback loop of lnc-ATB/miR-141-3p/TGF-β2 may be a potential therapeutic target for the treatment of GC.

  17. Photoelectric characteristics of CH3NH3PbI3/p-Si heterojunction

    Science.gov (United States)

    Yamei, Wu; Ruixia, Yang; Hanmin, Tian; Shuai, Chen

    2016-05-01

    Organic-inorganic hybrid perovskite CH3NH3PbI3 film is prepared on p-type silicon substrate using the one-step solution method to form a CH3NH3PbI3/p-Si heterojunction. The film morphology and structure are characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The photoelectric properties of the CH3NH3PbI3/p-Si heterojunction are studied by testing the current-voltage (I-V) with and without illumination and capacitance-voltage (C-V) characteristics. It turns out from the I-V curve without illumination that the CH3NH3PbI3/p-Si heterojunction has a rectifier feature with the rectification ratio over 70 at the bias of ±5 V. Also, there appears a photoelectric conversion phenomenon on this heterojunction with a short circuit current (Isc) of 0.16 μA and an open circuit voltage (Voc) of about 10 mV The high frequency C-V characteristic of the Ag/CH3NH3PbI3/p-Si heterojunction turns out to be similar to that of the metal-insulator-semiconductor (MIS) structure, and a parallel translation of the C-V curve along the forward voltage axis is found. This parallel translation means the existence of defects at the CH3NH3PbI3/p-Si interface and positive fixed charges in the CH3NH3PbI3 layer. The defects at the interface of the CH3NH3PbI3/p-Si heterojunction result in the dramatic decline of the Voc. Besides, the C-V test of CH3NH3PbI3 film shows a non-linear dielectric property and the dielectric value is about 4.64 as calculated. Project supported by the Hebei Province Natural Science Foundation of China (No. F2014202184) and the Tianjin Natural Science Foundation of China (No. 15JCZDJC37800).

  18. Yeast Agp2p and Agp3p function as amino acid permeases in poor nutrient conditions.

    Science.gov (United States)

    Schreve, James L; Garrett, Jinnie M

    2004-01-16

    The gene AGP2 and the ORF YFL055w (here named AGP3) are classified as members of the yeast amino acid permease gene family. Analysis of the growth of multiply-mutant strains in which these genes are disrupted shows that both encode permeases capable of supplying branched chain, and other, amino acids as nitrogen source. Both Agp2p and Agp3p are low affinity permeases for leucine (Kmapp 0.2-0.5 mM) and are expressed at lower levels than other permeases on all media tested. Thus, it appears that these two permeases can function as low affinity, relatively non-specific, permeases with redundant functions in the cell. Transcription of AGP2 and AGP3 is very low but is increased in cells lacking other functional general amino acid permeases (Gap1p or Agp1p). These results suggest Agp2p and Agp3p function in amino acid transport when nitrogen sources are limiting and/or other permeases are inactive.

  19. Photoionization of ground and excited levels of P II

    Science.gov (United States)

    Nahar, Sultana N.

    2017-01-01

    Photoionization cross section (σPI) of P II, (hν + P II → P III + e), from ground and a large number of excited levels are presented. The study includes the resonant structures and the characteristics of the background in photoionization cross sections. The present calculations were carried out in the Breit-Pauli R-matrix (BPRM) method that includes relativistic effects. The autoionizing resonances are delineated with a fine energy mesh to observe the fine structure effects. A singular resonance, formed by the coupling of channels in fine structure but not allowed in LS coupling, is seen at the ionization threshold of photoionization for the ground and many excited levels. The background cross section is seen enhanced compared to smooth decay for the excited levels. Examples are presented to illustrate the enhanced background cross sections at the energies of the core levels, 4P3/2 and 2D3/2, that are allowed for electric dipole transitions by the core ground level 2 P1/2o. In addition strong Seaton or photo-excitation-of-core (PEC) resonances are found in the photoionization of single valence electron excited levels. Calculations used a close coupling wave function expansion that included 18 fine structure levels of core P III from configurations 3s23p, 3s3p2, 3s23d, 3s24s, 3s24p and 3p3. Photoionization cross sections are presented for all 475 fine structure levels of P II found with n ≤ 10 and l ≤ 9. The present results will provide high precision parameters of various applications involving this less studied ion.

  20. Class III PI 3-kinase is the main source of PtdIns3P substrate and membrane recruitment signal for PIKfyve constitutive function in podocyte endomembrane homeostasis.

    Science.gov (United States)

    Ikonomov, Ognian C; Sbrissa, Diego; Venkatareddy, Madhusudan; Tisdale, Ellen; Garg, Puneet; Shisheva, Assia

    2015-05-01

    The evolutionarily conserved PIKfyve, which synthesizes PtdIns5P from PtdIns, and PtdIns(3,5)P2 from PtdIns3P, requires PtdIns3P as both an enzyme substrate and a membrane recruitment signal. Whereas the PtdIns3P source is undetermined, class III PI3K (Vps34), the only evolutionarily conserved of the eight mammalian PI3Ks, is presumed as a main candidate. A hallmark of PIKfyve deficiency is formation of multiple translucent cytoplasmic vacuoles seen by light microscopy in cells cultured in complete media. Such an aberrant phenotype is often observed in cells from conditional Vps34 knockout (KO) mice. To clarify the mechanism of Vps34 KO-triggered vacuolation and the PtdIns3P source for PIKfyve functionality, here we have characterized a podocyte cell type derived from Vps34fl/fl mice, which, upon Cre-mediated gene KO, robustly formed cytoplasmic vacuoles resembling those in PikfyveKO MEFs. Vps34wt, expressed in Vps34KO podocytes restored the normal morphology, but only if the endogenous PIKfyve activity was intact. Conversely, expressed PIKfyvewt rescued completely the vacuolation only in PikfyveKO MEFs but not in Vps34KO podocytes. Analyses of phosphoinositide profiles by HPLC and localization patterns by a PtdIns3P biosensor revealed that Vps34 is the main supplier of localized PtdIns3P not only for PIKfyve activity but also for membrane recruitment. Concordantly, Vps34KO podocytes had severely reduced steady-state levels of both PtdIns(3,5)P2 and PtdIns5P, along with PtdIns3P. We further revealed a plausible physiologically-relevant Vps34-independent PtdIns3P supply for PIKfyve, operating through activated class I PI3Ks. Our data provide the first evidence that the vacuolation phenotype in Vps34KO podocytes is due to PIKfyve dysfunction and that Vps34 is a main PtdIns3P source for constitutive PIKfyve functionality.

  1. Repression of microRNA-768-3p by MEK/ERK signalling contributes to enhanced mRNA translation in human melanoma.

    Science.gov (United States)

    Jiang, C C; Croft, A; Tseng, H-Y; Guo, S T; Jin, L; Hersey, P; Zhang, X D

    2014-05-15

    Increased global protein synthesis and selective translation of mRNAs encoding proteins contributing to malignancy is common in cancer cells. This is often associated with elevated expression of eukaryotic translation initiation factor 4 (eIF4E), the rate-limiting factor of cap-dependent translation initiation. We report here that in human melanoma downregulation of miR-768-3p as a result of activation of the mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) pathway has an important role in the upregulation of eIF4E and enhancement in protein synthesis. Melanoma cells displayed increased nascent protein production and elevated eIF4E expression, which was associated with the downregulation of miR-768-3p that was predicted to target the 3'-untranslated region of the eIF4E mRNA. Overexpression of miR-768-3p led to the downregulation of the endogenous eIF4E protein, reduction in nascent protein synthesis and inhibition of cell survival and proliferation. These effects were efficiently reversed when eIF4E was co-overexpressed in melanoma cells. On the other hand, introduction of anti-miR-768-3p into melanocytes upregulated endogenous eIF4E protein expression and increased global protein synthesis. Downregulation of miR-768-3p appeared to be mediated by activation of the MEK/ERK pathway, in that treatment of BRAF(V600E) melanoma cells with the mutant BRAF inhibitor PLX4720 or exposure of either BRAF(V600E) or wild-type BRAF melanoma cells to the MEK inhibitor U0126 resulted in the upregulation of miR-768-3p and inhibition of nascent protein synthesis. This inhibition was partially blocked in cells cointroduced with anti-miR-768-3p. Significantly, miR-768-3p was similarly downregulated, which was inversely associated with the expression levels of eIF4E in fresh melanoma isolates. Taken together, these results identify downregulation of miR-768-3p and subsequent upregulation of eIF4E as an important mechanism in addition to

  2. Lifetime measurement of some excited states belonging to the 3p4nd ( = 4–6) configurationof ArII

    Indian Academy of Sciences (India)

    S Karmakar; M B Das

    2007-09-01

    The radiative lifetimes of eight levels belonging to the 3p4nd ( = 4–6) configuration of ArII have been measured using high frequency deflection technique together with a delayed coincidence single photon counting arrangement. Lifetimes of some of the levels have been measured for the first time. The results have been compared with other experimental and theoretical values.

  3. Verifier-based Password Authenticated 3P-EKE Protocol using PCLA keys

    Directory of Open Access Journals (Sweden)

    Archana Raghuvamshi

    2016-06-01

    Full Text Available This paper endeavors to present a novel framework for the generic structure of a verifier-based password authenticated Three-Party Encrypted Key Exchange (3P-EKE protocol which yields more efficient protocol than the ones knew before. A previous framework presented by Archana and Premchand is more secured against all types of attacks like password guessing, replay, pre-play, man-in-the-middle attack etc. But unfortunately, this protocol does not solve the problem of a server compromise. These proofs help as inspiration to search for another framework. The framework we offer produces more efficient 3P-EKE protocol, and, in addition, delivers perceptive clarification about the existing attacks that do not solve in the previous framework. Moreover, it allows direct change from a class of verge private-key encryption to a hybrid (symmetric & Asymmetric one without significant overhead.

  4. Mechanism and kinetics for the reaction of O(3P) with DMSO: A theoretical study

    Science.gov (United States)

    Mandal, Debasish; Bagchi, Sabyasachi; Das, Abhijit K.

    2012-11-01

    Mechanism and kinetics for the reaction of DMSO with O(3P) have been investigated by M06-2X/MG3S, CBS-QB3 and G4MP2 methods. Four possible reaction pathways are identified. Among them, the O(3P) addition to S-atom followed by CH3 elimination is almost exclusive. Four pre-reactive complexes have been located. AIM theory is used to determine the nature of interactions in these complexes. Considering the formation of pre-reactive complex, the rate constant for major pathway is calculated using transition state theory applied to a two-step mechanism. Enthalpies of formation at 298.15 K (ΔfH°298.15) have been calculated using the composite CBS-QB3, G4MP2 and G3B3 methods.

  5. Multi-reassortant G3P[3] group A rotavirus in a horseshoe bat in Zambia.

    Science.gov (United States)

    Sasaki, Michihito; Orba, Yasuko; Sasaki, Satoko; Gonzalez, Gabriel; Ishii, Akihiro; Hang'ombe, Bernard M; Mweene, Aaron S; Ito, Kimihito; Sawa, Hirofumi

    2016-10-01

    Group A rotavirus is a major cause of diarrhoea in humans, especially in young children. Bats also harbour group A rotaviruses, but the genetic backgrounds of bat rotavirus strains are usually distinct from those of human rotavirus strains. We identified a new strain of group A rotavirus in the intestinal contents of a horseshoe bat in Zambia. Whole genome sequencing revealed that the identified virus, named RVA/Bat-wt/ZMB/LUS12-14/2012/G3P[3], possessed the genotype constellation G3-P[3]-I3-R2-C2-M3-A9-N2-T3-E2-H3. Several genome segments of LUS12-14 were highly similar to those of group A rotaviruses identified from humans, cows and antelopes, indicating interspecies transmission of rotaviruses between bats and other mammals with possible multiple genomic reassortment events.

  6. MiR-223-3p as a Novel MicroRNA Regulator of Expression of Voltage-Gated K+ Channel Kv4.2 in Acute Myocardial Infarction

    Directory of Open Access Journals (Sweden)

    Xue Liu

    2016-06-01

    Full Text Available Background/Aims: Acute myocardial infarction (AMI is a devastating cardiovascular disease with a high rate of morbidity and mortality, partly due to enhanced arrhythmogenicity. MicroRNAs (miRNAs have been shown to participate in the regulation of cardiac ion channels and the associated arrhythmias. The purpose of this study was to test our hypothesis that miR-223-3p contributes to the electrical disorders in AMI via modulating KCND2, the gene encoding voltage-gated channel Kv4.2 that carries transient outward K+ current Ito. Methods: AMI model was established in male Sprague-Dawley (SD rats by left anterior descending artery (LAD ligation. Evans blue and TTC staining was used to measure infarct area. Ito was recorded in isolated ventricular cardiomyocytes or cultured neonatal rat ventricular cells (NRVCs by whole-cell patch-clamp techniques. Western blot analysis was employed to detect the protein level of Kv4.2 and real-time RT-PCR to determine the transcript level of miR-223-3p. Luciferase assay was used to examine the interaction between miR-223-3p and KCND2 in cultured NRVCs. Results: Expression of miR-223-3p was remarkably upregulated in AMI relative to sham control rats. On the contrary, the protein level of Kv4.2 and Ito density were significantly decreased in AMI. Consistently, transfection of miR-223-3p mimic markedly reduced Kv4.2 protein level and Ito current in cultured NRVCs. Co-transfection of AMO-223-3p (an antisense inhibitor of miR-223-3p reversed the repressive effect of miR-223-3p. Luciferase assay showed that miR-223-3p, but not the negative control, substantially suppressed the luciferase activity, confirming the direct binding of miR-223-3p to the seed site within the KCND2 sequence. Finally, direct intramuscular injection of AMO-223-3p into the ischemic myocardium to knockdown endogenous miR-223-3p decreased the propensity of ischemic arrhythmias. Conclusions: Upregulation of miR-223-3p in AMI repressed the expression of

  7. Auger Spectra and Different Ionic Charges Following 3s, 3p and 3d Sub-Shells Photoionization of Kr Atoms

    Directory of Open Access Journals (Sweden)

    Yehia A. Lotfy

    2006-01-01

    Full Text Available The decay of inner-shell vacancy in an atom through radiative and non-radiative transitions leads to final charged ions. The de-excitation decay of 3s, 3p and 3d vacancies in Kr atoms are calculated using Monte-Carlo simulation method. The vacancy cascade pathway resulted from the de-excitation decay of deep core hole in 3s subshell in Kr atoms is discussed. The generation of spectator vacancies during the vacancy cascade development gives rise to Auger satellite spectra. The last transitions of the de-excitation decay of 3s, 3p and 3d holes lead to specific charged ions. Dirac-Fock-Slater wave functions are adapted to calculate radiative and non-radiative transition probabilities. The intensity of Kr^{4+} ions are high for 3s hole state, whereas Kr^{3+} and Kr^{2+} ions have highest intensities for 3p and 3d hole states, respectively. The present results of ion charge state distributions agree well with the experimental data.

  8. LiNbO3/p+n diode surface acoustic wave memory correlator

    Institute of Scientific and Technical Information of China (English)

    张朝; 水永安; 印建华

    1997-01-01

    A detailed theoretical analysis of strip-coupled LiNbO3/p+ n diode surface acoustic wave (SAW) memory correlator in the parametric mode is presented. The influence of some important factors on correlation output is analyzed and calculated, including the amplitudes of reference, read and write signal, duration of write signal and doping density of the diode array. The conclusions can be employed for the design of improved strip-coupled SAW memorycorrelators.

  9. Fracture toughness of Al-4%Mg/Al{sub 2}O{sub 3}p composites

    Energy Technology Data Exchange (ETDEWEB)

    Pestes, R.H. [Hewlett-Packard, Camus, WA 98607 (United States); Kamat, S.V. [Mechanical and Materials Engineering Department, Washington State University, Pullman, WA 99164-2920 (United States); Hirth, J.P. [Mechanical and Materials Engineering Department, Washington State University, Pullman, WA 99164-2920 (United States)

    1994-12-20

    Fracture toughness tests were carried out on Al-4%Mg/Al{sub 2}O{sub 3}p composites containing different sizes and volume fractions of alumina particulates. The results indicated that the fracture toughness was dependent on the interparticle spacing provided the particulate size was below a critical size. The critical particle size corresponds to the situation wherein the energy release rate on particulate cracking is sufficient to trigger dynamic unstable crack extension. ((orig.))

  10. Configuration interaction calculations of positron binding to Be({sup 3}P )

    Energy Technology Data Exchange (ETDEWEB)

    Bromley, M.W.J. [Department of Physics, San Diego State University, San Diego, CA 92182 (United States)]. E-mail: mbromley@physics.sdsu.edu; Mitroy, J. [Faculty of Technology, Charles Darwin University, Darwin, NT 0909 (Australia)]. E-mail: jxm107@rsphysse.anu.edu.au

    2006-06-15

    The configuration interaction method is applied to investigate the possibility of positron binding to the metastable beryllium (1s{sup 2}2s2p {sup 3}P ) state. The largest calculation obtained an estimated energy that was unstable by 0.00014 Hartree with respect to the Ps + Be{sup +}(2s) lowest dissociation channel. It is likely that positron binding to parent states with non-zero angular momentum is inhibited by centrifugal barriers.

  11. The Participative Design of an Endoscopy Facility using Lean 3P.

    Science.gov (United States)

    Smith, Iain

    2016-01-01

    In the UK, bowel cancer is the second largest cancer killer. Diagnosing people earlier can save lives but demand for endoscopies is increasing and this can put pressure on waiting times. To address this challenge, an endoscopy unit in North East England decided to improve their facilities to increase capacity and create environments that improve the experience of users. This presented a significant opportunity for step change improvement but also a problem in terms of creating designs that meet user requirements whilst addressing structural or space constraints. The Lean design process known as '3P' (standing for the production preparation process) was utilised as a participative design strategy to engage stakeholders in the design of the new department. This involved a time-out workshop (or 3P event) in which Lean and participative design tools were utilised to create an innovative design based on 'point of delivery' (POD) principles. The team created a design that demonstrated an increase in treatment room capacity by 25% and bed capacity by 70% whilst reducing travel distance for patients by 25.8% and staff by 27.1%. This was achieved with an increase in available space of only 13%. The Lean 3P method provided a structured approach for corporate and clinical staff to work together with patient representatives as cross-functional teams. This participative approach facilitated communication and learning between stakeholders about care processes and personal preferences. Lean 3P therefore appears to be a promising approach to improving the healthcare facilities design process to meet user requirements.

  12. Geomagnetism of earth's core

    Science.gov (United States)

    Benton, E. R.

    1983-01-01

    Instrumentation, analytical methods, and research goals for understanding the behavior and source of geophysical magnetism are reviewed. Magsat, launched in 1979, collected global magnetometer data and identified the main terrestrial magnetic fields. The data has been treated by representing the curl-free field in terms of a scalar potential which is decomposed into a truncated series of spherical harmonics. Solutions to the Laplace equation then extend the field upward or downward from the measurement level through intervening spaces with no source. Further research is necessary on the interaction between harmonics of various spatial scales. Attempts are also being made to analytically model the main field and its secular variation at the core-mantle boundary. Work is also being done on characterizing the core structure, composition, thermodynamics, energetics, and formation, as well as designing a new Magsat or a tethered satellite to be flown on the Shuttle.

  13. miR-142-3p prevents macrophage differentiation during cancer-induced myelopoiesis.

    Science.gov (United States)

    Sonda, Nada; Simonato, Francesca; Peranzoni, Elisa; Calì, Bianca; Bortoluzzi, Stefania; Bisognin, Andrea; Wang, Ena; Marincola, Francesco M; Naldini, Luigi; Gentner, Bernhard; Trautwein, Christian; Sackett, Sara Dutton; Zanovello, Paola; Molon, Barbara; Bronte, Vincenzo

    2013-06-27

    Tumor progression is accompanied by an altered myelopoiesis causing the accumulation of immunosuppressive cells. Here, we showed that miR-142-3p downregulation promoted macrophage differentiation and determined the acquisition of their immunosuppressive function in tumor. Tumor-released cytokines signaling through gp130, the common subunit of the interleukin-6 cytokine receptor family, induced the LAP∗ isoform of C/EBPβ transcription factor, promoting macrophage generation. miR-142-3p downregulated gp130 by canonical binding to its messenger RNA (mRNA) 3' UTR and repressed C/EBPβ LAP∗ by noncanonical binding to its 5' mRNA coding sequence. Enforced miR expression impaired macrophage differentiation both in vitro and in vivo. Mice constitutively expressing miR-142-3p in the bone marrow showed a marked increase in survival following immunotherapy with tumor-specific T lymphocytes. By modulating a specific miR in bone marrow precursors, we thus demonstrated the feasibility of altering tumor-induced macrophage differentiation as a potent tool to improve the efficacy of cancer immunotherapy.

  14. $^3P_2$-$^3F_2$ Pairing in Dense Neutron Matter The Spectrum of Solutions

    CERN Document Server

    Zverev, M V; Khodel, V A

    2003-01-01

    The $^3P_2$-$^3F_2$ pairing model is generally considered to provide an adequate description of the superfluid states of neutron matter at densities some 2-3 times that of saturated symmetrical nuclear matter. The problem of solving the system of BCS gap equations expressing the $^3P_2$-$^3F_2$ model is attacked with the aid of the separation approach. This method, developed originally for quantitative study of S-wave pairing in the presence of strong short-range repulsions, serves effectively to reduce the coupled, singular, nonlinear BCS integral equations to a set of coupled algebraic equations. For the first time, sufficient precision becomes accessible to resolve small energy splittings between the different pairing states. Adopting a perturbative strategy, we are able to identify and characterize the full repertoire of real solutions of the $^3P_2$-$^3F_2$ pairing model, in the limiting regime of small tensor-coupling strength. The P-F channel coupling is seen to lift the striking parametric degeneracie...

  15. Relative Abstract Nature of the Three Core Science Subjects at the Senior Secondary Level in Nigeria as Exemplified by Classroom Interaction Patterns

    Directory of Open Access Journals (Sweden)

    Emmanuel E Achor

    2011-06-01

    Full Text Available This study examined relative abstract nature of Biology, Chemistry and Physics offered at the senior secondary schools (SSS in Ankpa education zone of Kogi State of Nigeria based on the analysis of classroom interactions. In each of the three comparable public schools used, the same class of Senior Secondary 2 (SS 2 or 11th grade students were each taught Biology, Chemistry and Physics. In each school, reproduction, chemical kinetics and refraction were taught in Biology, Chemistry and Physics respectively. The researchers personally took record of interactions during the 9 periods (taught by 9 science teachers lasting for 35 minutes each using Flanders’ Interaction Analysis Categories (FIAC. Inter observer’s rater reliability was 0.69 using Scott’s Phi coefficient. Using a 10 by 10 matrix and percentage for final analysis, the extent of students’ participation in the lesson which its decreasing order was used to estimate the degree of the abstract nature or difficulty experienced in each subject was determined. The result revealed that the physical sciences were more abstract than the biological science with physics having the highest index. There was no close match between teachers’ level of motivation during the lessons and students’ participation except in Biology. Consequent upon these, it was recommended that chemistry and physics teachers should always ensure that there is a close match between cognitive ability of learners and cognitive demands of the subjects or lessons taught; that concrete teaching materials be used in the two more abstract subjects to reduce the formal reasoning or abstract requirements in the lessons to concrete demand levels, among others.

  16. Improving the Prediction of Prostate Cancer Overall Survival by Supplementing Readily Available Clinical Data with Gene Expression Levels of IGFBP3 and F3 in Formalin-Fixed Paraffin Embedded Core Needle Biopsy Material.

    Directory of Open Access Journals (Sweden)

    Zhuochun Peng

    Full Text Available A previously reported expression signature of three genes (IGFBP3, F3 and VGLL3 was shown to have potential prognostic value in estimating overall and cancer-specific survivals at diagnosis of prostate cancer in a pilot cohort study using freshly frozen Fine Needle Aspiration (FNA samples.We carried out a new cohort study with 241 prostate cancer patients diagnosed from 2004-2007 with a follow-up exceeding 6 years in order to verify the prognostic value of gene expression signature in formalin fixed paraffin embedded (FFPE prostate core needle biopsy tissue samples. The cohort consisted of four patient groups with different survival times and death causes. A four multiplex one-step RT-qPCR test kit, designed and optimized for measuring the expression signature in FFPE core needle biopsy samples, was used. In archive FFPE biopsy samples the expression differences of two genes (IGFBP3 and F3 were measured. The survival time predictions using the current clinical parameters only, such as age at diagnosis, Gleason score, PSA value and tumor stage, and clinical parameters supplemented with the expression levels of IGFBP3 and F3, were compared.When combined with currently used clinical parameters, the gene expression levels of IGFBP3 and F3 are improving the prediction of survival time as compared to using clinical parameters alone.The assessment of IGFBP3 and F3 gene expression levels in FFPE prostate cancer tissue would provide an improved survival prediction for prostate cancer patients at the time of diagnosis.

  17. Dual-core antiresonant hollow core fibers.

    Science.gov (United States)

    Liu, Xuesong; Fan, Zhongwei; Shi, Zhaohui; Ma, Yunfeng; Yu, Jin; Zhang, Jing

    2016-07-25

    In this work, dual-core antiresonant hollow core fibers (AR-HCFs) are numerically demonstrated, based on our knowledge, for the first time. Two fiber structures are proposed. One is a composite of two single-core nested nodeless AR-HCFs, exhibiting low confinement loss and a circular mode profile in each core. The other has a relatively simple structure, with a whole elliptical outer jacket, presenting a uniform and wide transmission band. The modal couplings of the dual-core AR-HCFs rely on a unique mechanism that transfers power through the air. The core separation and the gap between the two cores influence the modal coupling strength. With proper designs, both of the dual-core fibers can have low phase birefringence and short modal coupling lengths of several centimeters.

  18. Spin-orbit and rotational couplings in radiative association of C(3P) and N(4S) atoms.

    Science.gov (United States)

    Antipov, Sergey V; Gustafsson, Magnus; Nyman, Gunnar

    2011-11-14

    The role of spin-orbit and rotational couplings in radiative association of C((3)P) and N((4)S) atoms is investigated. Couplings among doublet electronic states of the CN radical are considered, giving rise to a 6-state model of the process. The solution of the dynamical problem is based on the L(2) method, where a complex absorbing potential is added to the Hamiltonian operator in order to treat continuum and bound levels in the same manner. Comparison of the energy-dependent rate coefficients calculated with and without spin-orbit and rotational couplings shows that the couplings have a strong effect on the resonance structure and low-energy baseline of the rate coefficient.

  19. miR-483-3p plays an oncogenic role in esophageal squamous cell carcinoma by targeting tumor suppressor EI24.

    Science.gov (United States)

    Ma, Jiaojiao; Hong, Liu; Xu, Guanghui; Hao, Junfeng; Wang, Rui; Guo, Hao; Liu, Jinqiang; Zhang, Yujie; Nie, Yongzhan; Fan, Daiming

    2016-04-01

    microRNAs (miRNAs), through negatively regulating their target genes, influence the development and progression of many cancers. Previously, we found miR-483 was overexpressed in esophageal squamous cell carcinoma (ESCC) tissues, and its overexpression was negatively correlated with the prognosis and positively correlated with multidrug resistance of ESCC, but whether it could affect the biological role of proliferation and migration in ESCC cell lines is unknown. In the present study, we found miR-483-3p was overexpressed in ESCC cell lines as compared with the normal esophageal squamous epithelial cell line. Functional experiments in vitro showed that miR-483-3p could promote the proliferation, migration, transformation of cell cycle from G1 phase to G2 phase of ESCC cells and could inhibit cells' sensitivity to chemotherapy drugs. Nude mouse tumorigenicity assay indicated that miR-483-3p could promote the growth of ESCC cells in vivo. Western blot assay showed that ectopic expression of miR-483-3p in ESCC cells could downregulate the protein level of etoposide induced 2.4 (EI24), which is a tumor suppressor and has not been reported in ESCC. Luciferase reporter assay demonstrated that EI24 was a direct target of miR-483-3p. Collectively, our study demonstrated that miR-483-3p could promote ESCC progression at least in part through directly targeting EI24, supplying a potential strategy for miRNA-based ESCC therapy.

  20. The human core of the shared socioeconomic pathways: Population scenarios by age, sex and level of education for all countries to 2100.

    Science.gov (United States)

    Kc, Samir; Lutz, Wolfgang

    2017-01-01

    This paper applies the methods of multi-dimensional mathematical demography to project national populations based on alternative assumptions on future, fertility, mortality, migration and educational transitions that correspond to the five shared socioeconomic pathways (SSP) storylines. In doing so it goes a significant step beyond past population scenarios in the IPCC context which considered only total population size. By differentiating the human population not only by age and sex-as is conventionally done in demographic projections-but also by different levels of educational attainment the most fundamental aspects of human development and social change are being explicitly addressed through modeling the changing composition of populations by these three important individual characteristics. The scenarios have been defined in a collaborative effort of the international Integrated Assessment Modeling community with the medium scenario following that of a major new effort by the Wittgenstein Centre for Demography and Global Human Capital (IIASA, OEAW, WU) involving over 550 experts from around the world. As a result, in terms of total world population size the trajectories resulting from the five SSPs stay very close to each other until around 2030 and by the middle of the century already a visible differentiation appears with the range between the highest (SSP3) and the lowest (SSP1) trajectories spanning 1.5 billion. The range opens up much more with the SSP3 reaching 12.6 billion in 2100 and SSP1 falling to 6.9 billion which is lower than today's world population.

  1. Novel electrochemical biosensor based on PVP capped CoFe2O4@CdSe core-shell nanoparticles modified electrode for ultra-trace level determination of rifampicin by square wave adsorptive stripping voltammetry.

    Science.gov (United States)

    Asadpour-Zeynali, Karim; Mollarasouli, Fariba

    2017-06-15

    This work introduces a new electrochemical sensor based on polyvinyl pyrrolidone capped CoFe2O4@CdSe core-shell modified electrode for a rapid detection and highly sensitive determination of rifampicin (RIF) by square wave adsorptive stripping voltammetry. The new PVP capped CoFe2O4@CdSe with core-shell nanostructure was synthesized by a facile synthesis method for the first time. PVP can act as a capping and etching agent for protection of the outer surface nanoparticles and formation of a mesoporous shell, respectively. Another important feature of this work is the choice of the ligand (1,10-phenanthroline) for precursor cadmium complex that works as a chelating agent in order to increase optical and electrical properties and stability of prepared nanomaterial. The nanoparticles have been characterized by field emission scanning electron microscopy (FESEM), transmission electron microscope (TEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), UV-vis, photoluminescence (PL) spectroscopy, FT-IR, and cyclic voltammetry techniques. The PL spectroscopy study of CoFe2O4@CdSe has shown significant PL quenching by the formation of CoFe2O4 core inside CdSe, this shows that CoFe2O4 NPs are efficient electron acceptors with the CdSe. It is clearly observed that the biosensor can significantly enhance electrocatalytic activity towards the oxidation of RIF, under the optimal conditions. The novelty of this work arises from the new synthesis method for the core-shell of CoFe2O4@CdSe. Then, the novel electrochemical biosensor was fabricated for ultra-trace level determination of rifampicin with very low detection limit (4.55×10(-17)M) and a wide linear range from 1.0×10(-16) to 1.0×10(-7)M. The fabricated biosensor showed high sensitivity and selectivity, good reproducibility and stability. Therefore, it was successfully applied for the determination of ultra-trace RIF amounts in biological and pharmaceutical samples with satisfactory recovery data.

  2. MiR-490-3p sensitizes ovarian cancer cells to cisplatin by directly targeting ABCC2

    Science.gov (United States)

    Tian, Jing; Xu, Yan-Ying; Li, Lian; Hao, Quan

    2017-01-01

    Cisplatin (CDDP) resistance becomes a large obstacle of the beneficial therapy for patients with ovarian cancer. MicroRNAs (miRNAs) act as post-transcriptional regulators of multiple genes’ expression and have been reported to be involved in multi-drug resistance. The purpose of this study was to determine the roles and molecular mechanism of miR-490-3p in the CDDP resistance in ovarian cancer. We found that miR-490-3p was downregulated in CDDP-resistant OVCAR3/CDDP and SKOV3/CDDP cells, which was due to the hypermethylation of miR-490-3p promoter. Functional studies demonstrated that miR-490-3p increased the cell response to CDDP in OVCAR3, SKOV3 and CDDP-resistant cells, while miR-490-3p inhibition resulted in opposite effects. Luciferase assay, real-time PCR and Western blot as well as immunohistochemistry validated that ABCC2 was a direct target of miR-490-3p and miR-490-3p downregulated ABCC2 expression via binding to its 3’UTR. Importantly, silencing of ABCC2 alleviated CDDP resistance induced by miR-490-3p inhibition, while ABCC2 overexpression restored CDDP resistance inhibited by miR-490-3p. In vivo study showed that miR-490-3p enhanced the cytotoxicity of CDDP. Finally, we found that miR-490-3p was downregulated in CDDP-resistant ovarian cancer tissues, while ABCC2 was upregulated. In conclusion, our data indicate that miR-490-3p enhances CDDP sensitivity of ovarian cancer cells through downregulating ABCC2 expression, and suggest that delivery of miR-490-3p might be a potential therapeutic strategy for patients with CDP-resistant ovarian cancer.

  3. PI3P 和 NO 在 UV-B 诱导蚕豆气孔关闭中的关系%The interactions of PI3P and NO in UV-B-induced stomatal closure of broad bean

    Institute of Scientific and Technical Information of China (English)

    樊彩明; 王静; 胡洁; 贺军民

    2015-01-01

    By stomatal bioassay and measurement of endogenous nitric oxide (NO)level in guard cells,the interactions of NO and phosphatidylinositol 3-phosphate (PI3P)[the product of phos-phatidylinositol 3-kinase (PI3K)]in UV-B-induced stomatal closure were studied in the epidermal strips of abaxial surface of broad bean(Vicia faba L.)leaves.The results showed that both the NO production in guard cells and stomatal closure induced by UV-B were significantly inhibited by PI3K inhibitors wortmannin (WM)and LY294002 (LY).Meanwhile,exogenous NO-relea-sing compound sodium nitroprusside (SNP)could completely reverse the inhibitory effect of LY and WM on the UV-B-induced stomatal closure,and WM and LY could not inhibit exogenous SNP-induced stomatal closure of broad bean.These results indicate that PI3P acts upstream of NO in the signal transduction pathway of UV-B-induced stomatal closure of broad bean.%以蚕豆(Vicia faba L.)叶片下表皮为材料,结合气孔开度分析和保卫细胞内源一氧化氮(NO)水平的测定,研究了 NO 和磷脂酰肌醇3-激酶(PI3K)的催化产物磷脂酰肌醇3-磷酸(PI3P)在紫外线 B(UV-B)诱导气孔关闭中的关系。结果显示:UV-B 辐射诱导蚕豆保卫细胞 NO 产生和气孔关闭的效应能被 PI3K 抑制剂沃曼青霉素(WM)和 LY294002(LY)显著抑制。同时,外源 NO释放剂硝普钠(SNP)处理能完全逆转 WM 和 LY 对 UV-B 诱导气孔关闭的抑制效应,而 WM 和LY 却不能抑制外源 SNP 诱导蚕豆气孔关闭的效应。结果说明,在 UV-B 诱导蚕豆气孔关闭的信号转导途径中 PI3P 的作用在 NO 上游。

  4. 75 FR 23574 - Airworthiness Directives; CFM International, S.A. CFM56-5B1/P, -5B2/P, -5B3/P, -5B3/P1, -5B4/P...

    Science.gov (United States)

    2010-05-04

    ..., S.A. CFM56-5B1/P, - 5B2/P, -5B3/P, -5B3/P1, -5B4/P, -5B5/P, -5B6/P, -5B7/P, -5B8/P, -5B9/P, -5B1/2P... Docket Operations office between 9 a.m. and 5 p.m., Monday through Friday, except Federal holidays. The... CFM International, S.A. CFM56-5B1/P, - 5B2/P, -5B3/P, -5B3/P1, -5B4/P, -5B5/P,......

  5. microRNA miR-142-3p Inhibits Breast Cancer Cell Invasiveness by Synchronous Targeting of WASL, Integrin Alpha V, and Additional Cytoskeletal Elements.

    Directory of Open Access Journals (Sweden)

    Alexander Schwickert

    Full Text Available MicroRNAs (miRNAs, micro ribonucleic acids are pivotal post-transcriptional regulators of gene expression. These endogenous small non-coding RNAs play significant roles in tumorigenesis and tumor progression. miR-142-3p expression is dysregulated in several breast cancer subtypes. We aimed at investigating the role of miR-142-3p in breast cancer cell invasiveness. Supported by transcriptomic Affymetrix array analysis and confirmatory investigations at the mRNA and protein level, we demonstrate that overexpression of miR-142-3p in MDA-MB-231, MDA-MB-468 and MCF-7 breast cancer cells leads to downregulation of WASL (Wiskott-Aldrich syndrome-like, protein: N-WASP, Integrin-αV, RAC1, and CFL2, molecules implicated in cytoskeletal regulation and cell motility. ROCK2, IL6ST, KLF4, PGRMC2 and ADCY9 were identified as additional targets in a subset of cell lines. Decreased Matrigel invasiveness was associated with the miR-142-3p-induced expression changes. Confocal immunofluorescence microscopy, nanoscale atomic force microscopy and digital holographic microscopy revealed a change in cell morphology as well as a reduced cell volume and size. A more cortical actin distribution and a loss of membrane protrusions were observed in cells overexpressing miR-142-3p. Luciferase activation assays confirmed direct miR-142-3p-dependent regulation of the 3'-untranslated region of ITGAV and WASL. siRNA-mediated depletion of ITGAV and WASL resulted in a significant reduction of cellular invasiveness, highlighting the contribution of these factors to the miRNA-dependent invasion phenotype. While knockdown of WASL significantly reduced the number of membrane protrusions compared to controls, knockdown of ITGAV resulted in a decreased cell volume, indicating differential contributions of these factors to the miR-142-3p-induced phenotype. Our data identify WASL, ITGAV and several additional cytoskeleton-associated molecules as novel invasion-promoting targets of miR-142-3

  6. Mutation in the splicing factor Hprp3p linked to retinitis pigmentosa impairs interactions within the U4/U6 snRNP complex.

    Science.gov (United States)

    Gonzalez-Santos, Juana Maria; Cao, Huibi; Duan, Rongqi Cathleen; Hu, Jim

    2008-01-15

    Mutations in PRPF3, a gene encoding the essential pre-mRNA splicing factor Hprp3p, have been identified in patients with autosomal dominant retinitis pigmentosa type 18 (RP18). Patients with RP18 have one of two single amino acid substitutions, Pro493Ser or Thr494Met, at the highly conserved Hprp3p C-terminal region. Pro493Ser occurs sporadically, whereas Thr494Met is observed in several unlinked RP families worldwide. The latter mutation also alters a potential recognition motif for phosphorylation by casein kinase II (CKII). To understand the molecular basis of RP18, we examined the consequences of Thr494Met mutation on Hprp3p molecular interactions with components of the U4/U6.U5 small nuclear ribonucleoprotein particles (snRNPs) complex. Since numerous mutations causing human diseases change pre-mRNA splice sites, we investigated whether Thr494Met substitution affects the processing of PRPF3 mRNA. We found that Thr494Met does not affect PRPF3 mRNA processing, indicating that the mutation may exert its effect primarily at the protein level. We used small hairpin RNAs to specifically silence the endogenous PRPF3 while simultaneously expressing HA-tagged Thr494Met. We demonstrated that the C- but not N-terminal region of Hprp3p is indeed phosphorylated by CKII in vitro and in cells. CKII-mediated Hprp3p phosphorylation was significantly reduced by Thr494Met mutation. Consequently, the Hprp3p C-terminal region is rendered partially defective in its association with itself, Hprp4p, and U4/U6 snRNA. Our findings provide new insights into the biology of Hprp3p and suggest that the loss of Hprp3p phosphorylation at Thr494 is a key step for initiating Thr494Met aberrant interactions within U4/U6 snRNP complex and that these are likely linked to the RP18 phenotype.

  7. The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.

    Science.gov (United States)

    Chen, Peng-Hsu; Chang, Cheng-Kuei; Shih, Chwen-Ming; Cheng, Chia-Hsiung; Lin, Cheng-Wei; Lee, Chin-Cheng; Liu, Ann-Jeng; Ho, Kuo-Hao; Chen, Ku-Chung

    2016-11-01

    Xanthohumol (XN), a prenylated chalcone extracted from hop plant Humulus lupulus L. (Cannabaceae), has potential for cancer therapy, including gliomas. Micro (mi)RNAs are small noncoding RNAs that control gene expression. Several miRNAs have been identified to participate in regulating glioma development. However, no studies have demonstrated whether miRNA is involved in XN cytotoxicity resulting in glioma cell death. This study investigated the effects of XN-mediated miRNA expression in activating apoptotic pathways in glioblastoma U87 MG cells. First, we found that XN significantly reduced cell viability and induced apoptosis via pro-caspase-3/8 cleavage and poly(ADP ribose) polymerase (PARP) degradation. We also identified that pro-caspase-9 cleavage, Bcl2 family expression changes, mitochondrial dysfunction, and intracellular ROS generation also participated in XN-induced glioma cell death. With a microarray analysis, miR-204-3p was identified as the most upregulated miRNA induced by XN cytotoxicity. The extracellular signal-regulated kinase (ERK)/c-Fos pathway was validated to participate in XN-upregulated miR-204-3p expression. With a promoter assay and ChIP analysis, we found that c-Fos dose-dependently bound to the miR-204-3p gene promoter region. Furthermore, miR-204-3p levels decreased in several glioma cell lines compared to astrocytes. Overexpression of miR-204-3p enhanced glioma cell apoptosis. IGFBP2, an upregulated regulator of glioma proliferation, was validated by a TCGA analysis as a direct target gene of miR-204-3p. XN's inhibition of the IGFBP2/AKT/Bcl2 pathway via miR-204-3p targeting played a critical role in mediating glioma cell death. These results emphasized that the XN-mediated miR-204-3p network may provide novel therapeutic strategies for future glioblastoma therapy and drug development.

  8. Animal MRI Core

    Data.gov (United States)

    Federal Laboratory Consortium — The Animal Magnetic Resonance Imaging (MRI) Core develops and optimizes MRI methods for cardiovascular imaging of mice and rats. The Core provides imaging expertise,...

  9. The dielectronic satellites to the 2s-3p Ne-like krypton resonance lines

    Energy Technology Data Exchange (ETDEWEB)

    Khakhalin, S.Ya. (MISDC, NPO ' ' VNIIFTRI' ' , Mendeleevo (Russian Federation)); Dyakin, V.M. (MISDC, NPO ' ' VNIIFTRI' ' , Mendeleevo (Russian Federation)); Faenov, A.Ya. (MISDC, NPO ' ' VNIIFTRI' ' , Mendeleevo (Russian Federation)); Fiedorowicz, H. (Inst. of Optoelectronics, Warsaw (Poland)); Bartnik, A. (Inst. of Optoelectronics, Warsaw (Poland)); Parys, P. (Inst. of Plasma Physics and Laser Microfusion, Warsaw (Poland)); Nilsen, J. (Lawrence Livermore National Lab., Livermore, CA (United States)); Osterheld, A. (Lawrence Livermore National Lab., Livermore, CA (United States))

    1994-08-01

    We present an analysis of dielectronic satellite spectra of 2p[sup 6]-2s2p[sup 6]3p Ne-like krypton resonance lines. The satellite structure was registered with high (better than [lambda]/[Delta][lambda] > 3500) spectral resolution in the emission of a laser irradiated gas puff target. We perform an unambiguous identification of satellite lines caused by radiative transitions from autoionizing states of sodium-like krypton ions. A total of about 20 spectral features are identified, most of them for the first time. Very good agreement between the satellite structure calculations and experimental emission spectra is obtained. (orig.).

  10. Absolute frequency measurement of the 1S0 - 3P0 transition of 171Yb

    CERN Document Server

    Pizzocaro, Marco; Rauf, Benjamin; Bregolin, Filippo; Milani, Gianmaria; Clivati, Cecilia; Costanzo, Giovanni A; Levi, Filippo; Calonico, Davide

    2016-01-01

    We report the absolute frequency measurement of the unperturbed transition 1S0 - 3P0 at 578 nm in 171Yb realized in an optical lattice frequency standard. The absolute frequency is measured 518 295 836 590 863.55(28) Hz relative to a cryogenic caesium fountain with a fractional uncertainty of 5.4x10-16 . This value is in agreement with the ytterbium frequency recommended as a secondary representation of the second in the International System of Units.

  11. Pulsar Spin-Down by 3P2 Superfluid Neutron with Field Decay

    Institute of Scientific and Technical Information of China (English)

    LUO Xin-Lian; PENG Qiu-He; CHOU Chih-Kang

    2003-01-01

    To describe pulsar spin-down, we present a simple combined torque model that takes into account both the standard magnetic dipole radiation and the electromagnetic radiation from the 3P2 superSuid vortex neutrons inside neutron star. Using an ordinary exponential model for the magnetic field decay, we derive an analytical formulae for pulsar evolution tracks. The pulsar evolution on the P-P diagram is quite different from that of the standard magnetic dipole radiation model, especially when the supernuid torque or Geld decay becomes dominant.

  12. Phase transitions in Cd3P2 at high pressures and high temperatures

    DEFF Research Database (Denmark)

    Yel'kin, F.S.; Sidorov, V.A.; Waskowska, A.;

    2008-01-01

    The high-pressure, high-temperature structural behaviour of Cd3P2 has been studied using electrical resistance measurements, differential thermal analysis, thermo baric analysis and X-ray diffraction. At room temperature, a phase transformation is observed at 4.0 GPa in compression....... The experimental zero-pressure bulk modulus of the low-pressure phase is 64.7(7) GPa, which agrees quite well with the calculated value of 66.3 GPa using the tight-binding linear muffin-tin orbital method within the local density approximation. Tentatively, the high-pressure phase has an orthorhombic crystal...

  13. Transition probabilities and lifetimes of the low-lying levels of Fe XIV

    Institute of Scientific and Technical Information of China (English)

    Fan Jian-Zhong; Wang Qing-Min; Chang Zhi-Wei; Dong Chen-Zhong

    2012-01-01

    The multi-configuration Dirac-Fock method is employed to calculate the transition energies,probabilities,and oscillator strengths for electric dipole allowed (E1) and forbidden (M1,E2,M2) lines for the 3s23p,3s3p2,3s23d,3p3,and 3s3p3d configurations of Fe XIV.The lifetimes of all 40 levels of these low-lying configurations are also derived.The valence-valence and core-valence correlation effects are accounted for in a systematic way.Breit interactions and quantum electrodynamics (QED) effects are estimated in subsequent relativistic configuration interaction (CI)calculations.The present results are in good agreement with other available theoretical and experimental values,and therefore can be used for the further astrophysical investigations.

  14. Repression of mitochondrial translation, respiration and a metabolic cycle-regulated gene, SLF1, by the yeast Pumilio-family protein Puf3p.

    Directory of Open Access Journals (Sweden)

    Marc Chatenay-Lapointe

    Full Text Available Synthesis and assembly of the mitochondrial oxidative phosphorylation (OXPHOS system requires genes located both in the nuclear and mitochondrial genomes, but how gene expression is coordinated between these two compartments is not fully understood. One level of control is through regulated expression mitochondrial ribosomal proteins and other factors required for mitochondrial translation and OXPHOS assembly, which are all products of nuclear genes that are subsequently imported into mitochondria. Interestingly, this cadre of genes in budding yeast has in common a 3'-UTR element that is bound by the Pumilio family protein, Puf3p, and is coordinately regulated under many conditions, including during the yeast metabolic cycle. Multiple functions have been assigned to Puf3p, including promoting mRNA degradation, localizing nucleus-encoded mitochondrial transcripts to the outer mitochondrial membrane, and facilitating mitochondria-cytoskeletal interactions and motility. Here we show that Puf3p has a general repressive effect on mitochondrial OXPHOS abundance, translation, and respiration that does not involve changes in overall mitochondrial biogenesis and largely independent of TORC1-mitochondrial signaling. We also identified the cytoplasmic translation factor Slf1p as yeast metabolic cycle-regulated gene that is repressed by Puf3p at the post-transcriptional level and promotes respiration and extension of yeast chronological life span when over-expressed. Altogether, these results should facilitate future studies on which of the many functions of Puf3p is most relevant for regulating mitochondrial gene expression and the role of nuclear-mitochondrial communication in aging and longevity.

  15. Hepatitis B virus basal core promoter mutations A1762T/G1764A are associated with genotype C and a low serum HBsAg level in chronically-infected HBeAg-positive Chinese patients.

    Science.gov (United States)

    Yan, Chun-Hui; Zhao, Cheng-Yu; Ding, Hai; Peng, Ya-Qin; Jin, Peng-Yuan; Yan, Ling; Zhuang, Hui; Li, Tong

    2012-11-01

    The present study was aimed to obtain baseline information of basal core promoter A1762T/G1764A and precore G1896A mutations of hepatitis B virus (HBV) in 192 HBeAg-positive chronically-infected Chinese patients, who were potential candidates for antiviral treatment. The detection of these mutations (including minor mutant subpopulations) was achieved by direct sequencing, whose sensitivity for minor mutant subpopulations identification was confirmed by clone sequencing. Patients enrolled were infected with either genotype B (46.35%) or C (53.65%) HBV identified by routine tests in our laboratory. The A1762T/G1764A or G1896A mutations were detected in 125specimens (125/192, 65.10%), in which 77 (77/125, 61.60%) existed as subpopulations. The A1762T/G1764A mutations were found to be more prevalent in genotype C than that in genotype B HBV [62.14% (64/103) vs. 20.22% (18/89), Pgenotypes. The emergence of A1762T/G1764A mutations was also found to be associated with an older age, an elevated ALT/AST level, and a lower HBsAg level in serum [wild-type vs. mutant: 4.57 (3.46-5.42) vs. 3.93 (2.51-5.36), Pgenotype C and a low serum HBsAg level in chronically-infected HBeAg-positive Chinese patients.

  16. Microarray based analysis of an inherited terminal 3p26.3 deletion, containing only the CHL1 gene, from a normal father to his two affected children

    Directory of Open Access Journals (Sweden)

    Lerone Margherita

    2011-04-01

    Full Text Available Abstract Background terminal deletions of the distal portion of the short arm of chromosome 3 cause a rare contiguous gene disorder characterized by growth retardation, developmental delay, mental retardation, dysmorphisms, microcephaly and ptosis. The phenotype of individuals with deletions varies from normal to severe. It was suggested that a 1,5 Mb minimal terminal deletion including the two genes CRBN and CNTN4 is sufficient to cause the syndrome. In addition the CHL1 gene, mapping at 3p26.3 distally to CRBN and CNTN4, was proposed as candidate gene for a non specific mental retardation because of its high level of expression in the brain. Methods and Results we describe two affected siblings in which array-CGH analysis disclosed an identical discontinuous terminal 3p26.3 deletion spanning less than 1 Mb. The deletion was transmitted from their normal father and included only the CHL1 gene. The two brothers present microcephaly, light mental retardation, learning and language difficulties but not the typical phenotype manifestations described in 3p- syndrome. Conclusion a terminal 3p26.3 deletion including only the CHL1 gene is a very rare finding previously reported only in one family. The phenotype of the affected individuals in the two families is very similar and the deletion has been inherited from an apparently normal parent. As already described for others recurrent syndromes with variable phenotype, these findings are challenging in genetic counselling because of an evident variable penetrance.

  17. Skin Transfection Patterns and Expression Kinetics of Electroporation-Enhanced Plasmid Delivery Using the CELLECTRA-3P, a Portable Next-Generation Dermal Electroporation Device.

    Science.gov (United States)

    Amante, Dinah H; Smith, Trevor R F; Mendoza, Janess M; Schultheis, Katherine; McCoy, Jay R; Khan, Amir S; Sardesai, Niranjan Y; Broderick, Kate E

    2015-08-01

    The CELLECTRA-3P dermal electroporation device (Inovio Pharmaceuticals, Plymouth Meeting, PA) has been evaluated in the clinic and shown to enhance the delivery of an influenza DNA vaccine. To understand the mechanism by which this device aids in enhancing the host immune response to DNA vaccines we investigated the expression kinetics and localization of a reporter plasmid (pGFP) delivered via the CELLECTRA-3P. Histological analysis revealed green fluorescent protein (GFP) expression as early as 1 hr posttreatment in the epidermal and dermal layers, and as early as 2 hr posttreatment in the subdermal layers. Immunofluorescence techniques identified keratinocytes, fibrocytes, dendritic-like cells, adipocytes, and myocytes as the principal cell populations transfected. We proceeded to demonstrate elicitation of robust host immune responses after plasmid DNA (pDNA) vaccination. In guinea pigs equivalent humoral (antibody binding titers) immune responses were observed between protocols using either CELLECTRA-3P or intramuscular electroporation to deliver the DNA vaccine. In nonhuman primates, robust interferon-γ enzyme-linked immunospot and protective levels of hemagglutination inhibition titers after pDNA vaccination were observed in groups treated with the CELLECTRA-3P. In conclusion, these findings may assist in the future to design efficient, tolerable DNA vaccination strategies for the clinic.

  18. Metalloid tolerance based on phytochelatins is not functionally equivalent to the arsenite transporter Acr3p.

    Science.gov (United States)

    Wysocki, Robert; Clemens, Stephan; Augustyniak, Daria; Golik, Pawel; Maciaszczyk, Ewa; Tamás, Markus J; Dziadkowiec, Dorota

    2003-05-01

    Active transport of metalloids by Acr3p and Ycf1p in Saccharomyces cerevisiae and chelation by phytochelatins in Schizosaccharomyces pombe, nematodes, and plants represent distinct strategies of metalloid detoxification. In this report, we present results of functional comparison of both resistance mechanisms. The S. pombe and wheat phytochelatin synthase (PCS) genes, when expressed in S. cerevisiae, mediate only modest resistance to arsenite and thus cannot functionally compensate for Acr3p. On the other hand, we show for the first time that phytochelatins also contribute to antimony tolerance as PCS fully complement antimonite sensitivity of ycf1Delta mutant. Remarkably, heterologous expression of PCS sensitizes S. cerevisiae to arsenate, while ACR3 confers much higher arsenic resistance in pcsDelta than in wild-type S. pombe. The analysis of PCS and ACR3 homologues distribution in various organisms and our experimental data suggest that separation of ACR3 and PCS genes may lead to the optimal tolerance status of the cell.

  19. Bonding and Site Preferences in the New Quasi-Binary Zr 2.7Hf 11.3P 9

    Science.gov (United States)

    Kleinke, Holger; Franzen, Hugo F.

    1998-03-01

    The new quasi-binary Zr 2.7Hf 11.3P 9was synthesized by arc-melting of Zr, Hf, Co, and HfP in a ratio corresponding to the initial composition "Zr 2.25Hf 6.75Co 2P 4". Zr 2.7Hf 11.3P 9crystallizes in the Zr 14P 9structure type, which is unknown in the binary Hf/P system. The ideal orthorhombic lattice dimensions (space group Pnnm(No. 58), Z=4) were refined to a=16.640(7) Å, b=27.40(2) Å, c=3.619(1) Å, V=1650(2) Å 3. The structure consists of three-dimensional condensed one-, two-, and three-capped trigonal (Zr, Hf) 6P prisms, occurring with numerous short M- Mbonds ( M=Zr, Hf). Each of the 15 metal sites is statistically occupied by a mixture of Zr and Hf, which varies significantly from site to site. The Hf/Zr ratio in a given site depends on the M- Mand M-P interactions. The systematic increase of this ratio with increasing total bond order, as evaluated via Mulliken overlap populations and Pauling bond orders, can be understood based on the trend that Hf forms stronger M- Mand M-P bonds than Zr. As expected for a metal-rich phosphide, band structure calculations for the hypothetical "Hf 14P 9" carried out with the extended Hückel approximation result in a significant density of states at the Fermi level.

  20. MiR-519d-3p suppresses invasion and migration of trophoblast cells via targeting MMP-2.

    Directory of Open Access Journals (Sweden)

    Jie Ding

    Full Text Available Our study was approved by the Medical Ethics Committee of Tang Du Hospital, Fourth Military Medical University and complied strictly with national ethical guidelines. Preeclampsia (PE is a specific clinical disorder characterized by gestational hypertension and proteinuria and is a leading cause of maternal and perinatal mortality worldwide. The miR-519d-3p is upregulated in the maternal plasma of patients with PE which indicates a possible association between this microRNA and the pathogenesis of PE. No studies to date have addressed the effect of miR-519d-3p on the invasion and migration of trophoblast cells. In our study, we found that miR-519d-3p expression was elevated in placental samples from patients with PE. In vitro, overexpression of miR-519d-3p significantly inhibited trophoblast cell migration and invasion, whereas transfection of a miR-519d-3p inhibitor enhanced trophoblast cell migration and invasion. Luciferase assays confirmed that matrix metalloproteinase-2 (MMP-2 is a direct target of miR-519d-3p. Quantitative real-time PCR and western blot assays showed that overexpression of miR-519d-3p downregulated MMP-2 mRNA and protein expression. Knockdown of MMP-2 using a siRNA attenuated the increased trophoblast migration and invasion promoted by the miR-519d-3p inhibitor. In placentas from patients with PE or normal pregnancies, a negative correlation between the expression of MMP-2 and miR-519d-3p was observed using the Pearson correlation and linear regression analysis. Our present findings suggest that upregulation of miR-519d-3p may contribute to the development of PE by inhibiting trophoblast cell migration and invasion via targeting MMP-2; miR-519d-3p may represent a potential predictive and therapeutic target for PE.

  1. Up-regulation of miR-370-3p restores glioblastoma multiforme sensitivity to temozolomide by influencing MGMT expression.

    Science.gov (United States)

    Gao, Yong-Tao; Chen, Xiao-Bing; Liu, Hong-Lin

    2016-01-01

    MicroRNAs (miRNA) are believed to play an important role in glioblastoma multiforme (GBM)chemotherapy. Our study aims to investigate potential miRNA biomarkers in GBM. Sixty GBM patients, which were given temozolomide (TMZ) chemotherapy and recurrent radiotherapy, were recruited. miRNA array was performed in cancerous and in paired normal tissues. Microarray results were further validated by a quantitative real-time PCR in selected tissues and GBM cell lines. TMZ resistance cells were developed and cell proliferation along with colony formation assays was determined. Our study employed H2AX formation and flow cytometry to analyse the role of miRNA in DNA damage and apoptosis. Our study illustrated 16 miRNA in which 9 were up-regulated and 7 down-regulated. and their differential expression were demonstrated in a recurrent GBM tissue. Among them, miRNA-370-3p demonstrated the highest level of down- regulation in tissues and in TMZ resistance cells. miRNA-370-3p mimic increased its expression and sensitivity of GBM cells to TMZ by suppressing the self-reparative ability of tumour cell DNA. O(6)-methylguanine-DNA methyltransferase (MGMT) was identified as the direct target gene of miR-370-3p, and it was found to be inversely correlated with miR-370-3p expression in tissue samples obtained. Thus, our study demonstrated a critical clinical role of an up-regulated miR-370-3p expression in glioblastoma multiforme chemotherapy sensitivity.

  2. miR-337-3p and its targets STAT3 and RAP1A modulate taxane sensitivity in non-small cell lung cancers.

    Directory of Open Access Journals (Sweden)

    Liqin Du

    Full Text Available NSCLC (non-small cell lung cancer often exhibits resistance to paclitaxel treatment. Identifying the elements regulating paclitaxel response will advance efforts to overcome such resistance in NSCLC therapy. Using in vitro approaches, we demonstrated that over-expression of the microRNA miR-337-3p sensitizes NCI-H1155 cells to paclitaxel, and that miR-337-3p mimic has a general effect on paclitaxel response in NSCLC cell lines, which may provide a novel adjuvant strategy to paclitaxel in the treatment of lung cancer. By combining in vitro and in silico approaches, we identified STAT3 and RAP1A as direct targets that mediate the effect of miR-337-3p on paclitaxel sensitivity. Further investigation showed that miR-337-3p mimic also sensitizes cells to docetaxel, another member of the taxane family, and that STAT3 levels are significantly correlated with taxane resistance in lung cancer cell lines, suggesting that endogenous STAT3 expression is a determinant of intrinsic taxane resistance in lung cancer. The identification of a miR-337-3p as a modulator of cellular response to taxanes, and STAT3 and RAP1A as regulatory targets which mediate that response, defines a novel regulatory pathway modulating paclitaxel sensitivity in lung cancer cells, which may provide novel adjuvant strategies along with paclitaxel in the treatment of lung cancer and may also provide biomarkers for predicting paclitaxel response in NSCLC.

  3. miRNA array screening reveals cooperative MGMT-regulation between miR-181d-5p and miR-409-3p in glioblastoma.

    Science.gov (United States)

    Khalil, Susanna; Fabbri, Enrica; Santangelo, Alessandra; Bezzerri, Valentino; Cantù, Cinzia; Di Gennaro, Gianfranco; Finotti, Alessia; Ghimenton, Claudio; Eccher, Albino; Dechecchi, Maria; Scarpa, Aldo; Hirshman, Brian; Chen, Clark; Ferracin, Manuela; Negrini, Massimo; Gambari, Roberto; Cabrini, Giulio

    2016-05-10

    The levels of expression of O6-methylguanine-DNA methyltransferase (MGMT) are relevant in predicting the response to the alkylating chemotherapy in patients affected by glioblastoma. MGMT promoter methylation and the published MGMT regulating microRNAs (miRNAs) do not completely explain the expression pattern of MGMT in clinical glioblastoma specimens. Here we used a genome-wide microarray-based approach to identify MGMT regulating miRNAs. Our screen unveiled three novel MGMT regulating miRNAs, miR-127-3p, miR-409-3p, and miR-124-3p, in addition to the previously identified miR-181d-5p. Transfection of these three novel miRNAs into the T98G glioblastoma cell line suppressed MGMT mRNA and protein expression. However, their MGMT- suppressive effects are 30-50% relative that seen with miR-181d-5p transfection. In silico analyses of The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) revealed that miR-181d-5p is the only miRNA that consistently exhibited inverse correlation with MGMT mRNA expression. However, statistical models incorporating both miR-181d-5p and miR-409-3p expression better predict MGMT expression relative to models involving either miRNA alone. Our results confirmed miR-181d-5p as the key MGMT-regulating miRNA. Other MGMT regulating miRNAs, including the miR-409-3p identified in this report, modify the effect of miR-181d-5p on MGMT expression. MGMT expression is, thus, regulated by cooperative interaction between key MGMT-regulating miRNAs.

  4. The expression pattern of miR-142-3p and miR-145-3p in rat tissues%大鼠miR-142-3p、miR-145-3p表达模式分析

    Institute of Scientific and Technical Information of China (English)

    吴慧; 赵宇龙; 王艳红; 房兴堂; 陈宏; 张春雷

    2016-01-01

    Objective To screen and identify the key miRNAs during mammary gland development and milk secretion of rats. Methods Gene U6 was taken as interior label gene by real time-PCR to compare the differences of expression levels of miR-142-3p and miR-145-3p in the mammary gland, liver, heart, spleen, lung, kidney, ovary and uterus after 21 postpartum. Moreover, the expressions of miR-142-3p and miR-145-3p in different stages (1, 7 and 21 d) of lactation were summarized. Results There was significant difference in miR-142-3p in lactation 21 d between different tissues. The expression of miR-142-3p was significantly higher in mammary gland than that in heart, spleen, lung, kidney, ovary and uterus tissues, which was second only to the expression in liver (P0.05). Furthermore, the relative expression level of miR-142-3p was continuing downward continued to decline in breast at different stages of lactation, while the relative expression level of miR-145-3p was up-regulated after down-regulating. Conclusion miR-142-3p and miR-145-3p are differentially expressed in different tissues and physiological periods in rats. In addition, miR-142-3p can regulate the growth of mammary gland and the formation of lactation by targeting prolactin receptor (Prlr), miR-145-3p may have the same function with miR-145 and miR-145-5p.%目的:筛选并鉴定对大鼠乳腺发育和泌乳调控起关键作用的微RNAs(miRNAs)。方法以U6为内参基因,运用实时荧光定量PCR,比较miR-142-3p、miR-145-3p在泌乳21 d大鼠乳腺、肝、心、脾、肺、肾、卵巢、子宫各器官的组织样品表达量的差异及不同泌乳阶段(1、7、21 d)乳腺组织miR-142-3p、miR-145-3p的表达规律。结果miR-142-3p在泌乳21 d各组织的表达存在差异,乳腺中的表达量显著高于心、脾、肺、肾、卵巢和子宫,仅次于肝中的表达量(P0.05)。乳腺组织在泌乳1、7、21 d比较,miR-142-3p的相对表达量持续下调,而miR-145-3p

  5. CHARACTERIZATION AND CHROMOSOMAL ASSIGNMENT OF YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN 3P13-P21-SPECIFIC SEQUENCE-TAGGED SITES

    NARCIS (Netherlands)

    MICHAELIS, SC; BARDENHEUER, W; LUX, A; SCHRAMM, A; GOCKEL, A; SIEBERT, R; WILLERS, C; SCHMIDTKE, K; TODT, B; VANDERHOUT, AH; BUYS, CHCM; HEPPELLPARTON, AC; RABBITTS, PH; UNGAR, S; SMITH, D; LEPASLIER, D; COHEN, D; OPALKA, B; SCHUTTE, J

    1995-01-01

    Human chromosomal region 3p12-p23 is proposed to harbor at least three tumor suppressor genes involved in the development of lung cancer, renal cell carcinoma, and other neoplasias. In order to identify one of these genes we defined sequence tagged sites (STSs) specific for 3p13-p24.2 by analyzing a

  6. Product lambda-doublet ratios for the O(3P) + D2 reaction: A mechanistic imprint

    CERN Document Server

    Jambrina, P G; Aldegunde, J; Brouard, M; Aoiz, F J

    2016-01-01

    In the last decade, the development of theoretical methods have allowed chemists to reproduce and explain almost all of the experimental data associated with elementary atom plus diatom collisions. However, there are still a few examples where theory cannot account yet for experimental results. This is the case for the preferential population of one of the $\\Lambda$-doublet states produced by chemical reactions. In particular, recent measurements of the OD($^2\\Pi$) product of the O($^3$P) + D$_2$ reaction have shown a clear preference for the $\\Pi(A')$ $\\Lambda$-doublet states, in apparent contradiction with {\\em ab initio} calculations, which predict a larger reactivity on the $A"$ potential energy surface. Here we present a method to calculate the $\\Lambda$-doublet ratio when concurrent potential energy surfaces participate in the reaction. It accounts for the experimental $\\Lambda$-doublet populations via explicit consideration of the stereodynamics of the process. Furthermore, our results demonstrate that...

  7. Superplasticity in an Aluminum Alloy 6061/A12O3p Composite

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The superplasticity of an Al2O3p/6061Al composite, fabricated by powder metallurgy techniques,has been investigated. Instead of any special thermomechanical processing or hot rolling, simple hot extrusion has been employed to obtain a fine grained structure before superplastic testing.Superplastic tensile tests were performed at strain rates ranging from 10-2 to 10-4 s-1 and at temperatures from 833 to 893 K. A maximum elongation of 200% was achieved at a temperature of 853 K and an initial strain rate of 1.67×10-3 s-1. The highest value obtained for the strain rate sensitivity index (m) was 0.32. Differential scanning calorimeter was used to ascertain the possibility of any partial melting in the vicinity of optimum superplastic temperature. These results suggested that no liquid phase existed where maximum elongation was achieved and deformation took place entirely in the solid state.

  8. Narrow-linewidth cooling of $^{6}$Li atoms using the 2S-3P transition

    CERN Document Server

    Chen, Hao-Ze; Wu, Yu-Ping; Liu, Xiang-Pei; Wang, Xiao-Qiong; Chen, Yu-Ao; Pan, Jian-Wei

    2016-01-01

    We report on a narrow-linewidth cooling of $^{6}$Li atoms using the $2S_{1/2}\\to 3P_{3/2}$ transition in the ultraviolet (UV) wavelength regime. By combining the traditional red magneto-optical trap (MOT) at 671 nm and the UV MOT at 323 nm, we obtain a cold sample of $1.3\\times10^9$ atoms with a temperature of 58 $\\mu$K. Furthermore, we demonstrate a high efficiency magnetic transport for $^{6}$Li atoms with the help of the UV MOT. Finally, we obtain $8.1\\times10^8$ atoms with a temperature of 296 $\\mu$K at a magnetic gradient of 198 G/cm in the science chamber with a good vacuum environment and large optical access.

  9. k-core covers and the core

    NARCIS (Netherlands)

    Sanchez-Rodriguez, E.; Borm, Peter; Estevez-Fernandez, A.; Fiestras-Janeiro, G.; Mosquera, M.A.

    2015-01-01

    This paper extends the notion of individual minimal rights for a transferable utility game (TU-game) to coalitional minimal rights using minimal balanced families of a specific type, thus defining a corresponding minimal rights game. It is shown that the core of a TU-game coincides with the core of

  10. Academic Rigor: The Core of the Core

    Science.gov (United States)

    Brunner, Judy

    2013-01-01

    Some educators see the Common Core State Standards as reason for stress, most recognize the positive possibilities associated with them and are willing to make the professional commitment to implementing them so that academic rigor for all students will increase. But business leaders, parents, and the authors of the Common Core are not the only…

  11. The − 5 A/G single-nucleotide polymorphism in the core promoter region of MT2A and its effect on allele-specific gene expression and Cd, Zn and Cu levels in laryngeal cancer

    Energy Technology Data Exchange (ETDEWEB)

    Starska, Katarzyna, E-mail: katarzyna.starska@umed.lodz.pl [I Department of Otolaryngology and Laryngological Oncology, Medical University of Łódź, Kopcinskiego 22, 90-153 Łódź (Poland); Krześlak, Anna; Forma, Ewa [Department of Cytobiochemistry, University of Łódź, Pomorska 142/143, 90-236 Łódź (Poland); Olszewski, Jurek [II Department of Otolaryngology and Laryngological Oncology, Medical University of Łódź, Żeromskiego 113, 90-549 Łódź (Poland); Morawiec-Sztandera, Alina [Department of Head and Neck Surgery, Medical University of Łódź, Paderewskiego 4, 93-509 Łódź (Poland); Aleksandrowicz, Paweł [Department of Otolaryngology and Laryngological Oncology, Medical University of Lublin, Jaczewskiego 8, 20-954 Lublin (Poland); Lewy-Trenda, Iwona [Department of Pathology, Medical University of Łódź, Pomorska 251, 92-213 Łódź (Poland); and others

    2014-10-15

    Metallothioneins (MTs) are low molecular weight, cysteine-rich heavy metal-binding proteins which participate in the mechanisms of Zn homeostasis, and protect against toxic metals. MTs contain metal-thiolate cluster groups and suppress metal toxicity by binding to them. The aim of this study was to determine the − 5 A/G (rs28366003) single-nucleotide polymorphism (SNP) in the core promoter region of the MT2A gene and to investigate its effect on allele-specific gene expression and Cd, Zn and Cu content in squamous cell laryngeal cancer (SCC) and non-cancerous laryngeal mucosa (NCM) as a control. The MT2A promoter region − 5 A/G SNP was determined by restriction fragment length polymorphism using 323 SCC and 116 NCM. MT2A gene analysis was performed by quantitative real-time PCR. The frequency of A allele carriage was 94.2% and 91.8% in SCC and NCM, respectively, while G allele carriage was detected in 5.8% and 8.2% of SCC and NCM samples, respectively. As a result, a significant association was identified between the − 5 A/G SNP in the MT2A gene with mRNA expression in both groups. Metal levels were analyzed by flame atomic absorption spectrometry. The significant differences were identified between A/A and both the A/G and G/G genotypes, with regard to the concentration of the contaminating metal. The Spearman rank correlation results showed that the MT2A expression and Cd, Zn, Cu levels were negatively correlated. Results obtained in this study suggest that − 5 A/G SNP in MT2A gene may have an effect on allele-specific gene expression and accumulation of metal levels in laryngeal cancer. - Highlights: • MT2A gene expression and metal content in laryngeal cancer tissues • Association between SNP (rs28366003) and expression of MT2A • Significant associations between the SNP and Cd, Zn and Cu levels • Negative correlation between MT2A gene expression and Cd, Zn and Cu levels.

  12. Theoretical study on the degradation reaction of octachlorinated dibenzo-p-dioxin with atomic oxygen O((3)P) in dielectric barrier discharge reactor.

    Science.gov (United States)

    Gong, Chen; Sun, Xiaomin; Zhang, Chenxi; Hu, Jingtian; Qi, Chuansong

    2014-11-01

    Dielectric barrier discharges (DBD) have been used in the degradation of dioxins due to the large number of excimers and free radicals produced in discharge process. In this article, the density functional theory (DFT) is used to study the degradation mechanism of octachlorinated dibenzo-p-dioxin (OCDD) with the atomic oxygen O((3)P) in DBD reactor. The reactants, intermediates, transition states and products are optimized at the MPWB1K/6-31+G(d,p) level. The vibrational frequencies have been calculated at the same level. The reaction pathways and mechanisms are analyzed in detail. The effect of removing the chlorine atom on environment also has been discussed.

  13. Multifunctional g3p-peptide tag for current phage display systems.

    Science.gov (United States)

    Beckmann, C; Haase, B; Timmis, K N; Tesar, M

    1998-03-15

    We have previously described a monoclonal antibody (mAb), 10C3, directed against the gene-3 protein (g3p) of filamentous phage M13, which was produced to study g3p fusion protein expression in Escherichia coli and its incorporation in the phage capsid [Tesar, M., Beckmann, C., Röttgen, P., Haase, B., Faude, U., Timmis, K., 1995. Monoclonal antibody against pIII of filamentous phage: an immunological tool to study pIII fusion protein expression in phage display systems. Immunology 1, 53-54]. In this study we report mapping of the antigenic epitope of the mAb 10C3, by means of short overlapping peptide-sequences [Frank, R., Overwin, H., 1996. Spot synthesis. In: Morris, G.E. (Ed.), Methods in Molecular Biology, Vol. 66: Epitope Mapping Protocols. Humana Press, Totowa, NJ, pp. 149-169.] comprising the C-terminal half of the g3-protein. A minimal recognizable peptide was found which is represented in the 11 amino acid sequence from positions 292 to 302 of g3p [Wezenbeek van, P.M.G.P., Hulsebos, T.J.M., Schoenmakers, J.G.G., 1980. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. Gene 11, 129-148]. In order to use the antibody also for detection and purification of recombinant proteins, such as single chain antibodies, the epitope was introduced as a tag sequence into the phagemid pHEN1 [Hoogenboom, H.R., Griffith, A.D., Johnson, K., Chiswell, D.J., Hudson, P., Winter, G., 1991. Multi-subunit proteins on the surface of the filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acid Res. 19, 4133-4137; Nissim, A., Hoogenboom, H.R., Tomlinson, I.M., Flynn, G., Midgley, C., Lane, D., Winter, G., 1994. Antibody fragments from a single pot phage display library as immunochemical reagents. EMBO J. 13 (3) 692-698]. Purified single chain antibodies containing this tag were detectable down to a concentration of 2 ng ml(-1) under non-denaturing conditions (ELISA) or 4 ng per lane on immunoblots

  14. miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200

    Science.gov (United States)

    Fang, Wang; Shu, Shan; Yongmei, Li; Endong, Zhu; Lirong, Yin; Bei, Sun

    2016-01-01

    Cervical cancer (CC) is a malignant solid tumor, which is one of the main causes of morbidity and mortality in women. Persistent High-risk human papillomavirus (hrHPV) infection is closely related to cervical cancer and autophagy has been suggested to inhibit viral infections. miRNAs have been reported to regulate autophagy in many solid tumors with many studies implicating miR-224-3p in the regulation of autophagy. In this study, we performed a miRNA microarray analysis on CC tissues and found that a large number of miRNAs with differential expressions in hrHPV-infected tissues. We identified miR-224-3p as a candidate miRNA selectively up regulated in HPV-infected tissues and cell lines. Further analysis revealed that miR-224-3p regulates autophagy in cervical cancer tissues and cell lines. While the overexpression of miR-224-3p inhibits autophagy in HPV-infected cells, knocking down endogenous miR-224-3p increases autophagy activity in the same cells. In addition, we found that miR-224-3p directly inhibits the expression of autophagy related gene, FAK family-interacting protein of 200 kDa (FIP200). In summary, we found that miR-224-3p regulates autophagy in hrHPV-induced cervical cancer cells through targeting FIP200 expression. PMID:27615604

  15. MicroRNA-338-3p suppresses tumor growth of esophageal squamous cell carcinoma in vitro and in vivo.

    Science.gov (United States)

    Li, Xinyu; Li, Zhihong; Yang, Guiyun; Pan, Zhenxiang

    2015-09-01

    Accumulating evidence has shown that microRNAs (miRNAs) are aberrantly expressed in human esophageal squamous cell carcinoma (ESCC) and are crucial in tumorigenesis, among which miR‑338‑3p has been examined to be downregulated in patients with ESCC. However, the role of miR‑338‑3p in ESCC remains to be elucidated. In the present study, the role of miR‑338‑3p on the growth and survival of an ESCC cell line was determined with several in vitro approaches and in nude mouse models. It was determined that miR‑338‑3p expression was frequently downregulated in ESCC tissue compared with corresponding adjacent non‑tumor tissue, and that its expression was significantly correlated with tumor stage and metastasis. Overexpression of miR‑338‑3p in ESCC cells suppressed cell proliferation, colony formation, migration and invasion, and induced cell arrest at the G0/G1 stage and cell apoptosis in vitro. In addition, it was demonstrated that overexpression of miR‑338‑3p significantly suppresses tumor growth of xenograft tumors in mice (PESCC, and its dysregulation may be involved in the initiation and development of human ESCC. In addition, it was suggested that miR‑338‑3p may be a potential therapeutic agent for treatment of ESCC.

  16. Drilling history core hole DC-8

    Energy Technology Data Exchange (ETDEWEB)

    1978-10-01

    Core hole DC-8 was completed in August, 1978 by Boyles Brothers Drilling Company, Spokane, Washington, under subcontract to Fenix and Scission, Inc. The hole was cored for the US Department of Energy and the Rockwell Hanford Operations' Basalt Waste Isolation Program. Fenix and Scisson, Inc. furnished the engineering, daily supervision of the core drilling activities, and geologic core logging for hole DC-8. Core hole DC-8 is located on the Hanford Site near the Wye Barricade and 50 feet northwest of rotary hole DC-7. The Hanford Site vation coordinates for DC-8 are North 14,955.94 feet and West 14,861.92 coordinates for DC-8 are North 14,955.94 feet and West 14,861.92 mean sea level. The purpose of core hole DC-8 was to core drill vertically through the basalt and interbed units for stratigraphic depth determination and core collection, and to provide a borehole for hydrologic testing and cross-hole seismic shear and pressure wave velocity studies with rotary hole DC-7. The total depth of core hole DC-8 was 4100.5 feet. Core recovery exceeded 97 percent of the total footage cored.

  17. Two distinct subtypes of hepatitis B virus-related acute liver failure are separable by quantitative serum immunoglobulin M anti-hepatitis B core antibody and hepatitis B virus DNA levels

    DEFF Research Database (Denmark)

    Dao, Doan Y; Hynan, Linda S; Yuan, He-Jun

    2012-01-01

    of immunoglobulin M (IgM) anti-hepatitis B core antibody (anti-HBc) titers and of HBV viral loads (VLs) might allow the separation of AHBV-ALF from CHBV-ALF. Of 1,602 patients with ALF, 60 met clinical criteria for AHBV-ALF and 27 for CHBV-ALF. Sera were available on 47 and 23 patients, respectively. A quantitative...... immunoassay was used to determine IgM anti-HBc levels, and real-time polymerase chain reaction (rtPCR) was used to determine HBV VLs. AHBV-ALFs had much higher IgM anti-HBc titers than CHBV-ALFs (signal-to-noise [S/N] ratio median: 88.5; range, 0-1,120 versus 1.3, 0-750; P ...) of the AHBV-ALF group had no hepatitis B surface antigen (HBsAg) detectable on admission to study, wheras no CHBV-ALF patients experienced HBsAg clearance. Rates of transplant-free survival were 33% (20 of 60) for AHBV-ALF versus 11% (3 of 27) for CHBV-ALF (P = 0.030). CONCLUSIONS: AHBV-ALF and CHBV...

  18. Radiationless S 1 → S 0 phenyl deactivation pathway: an investigation of iodine-marked bi-phenyl on a silicon surface by means of time resolved core-level photoelectron spectroscopy

    Science.gov (United States)

    Michelswirth, Martin; Dachraoui, Hatem; Mattay, Jochen; Heinzmann, Ulrich

    2012-02-01

    The S 1 → S 0 radiationless deactivation of iodine terminated bi-phenyl immobilized on a silicon surface was probed by analysing the I4d signature (BE: 45.6 eV, 47.3 eV) by means of High Harmonic Generation (HHG) based photoelectron spectroscopy. Modifications of the 4d5/2,3/2 spectroscopic contents spanning about 0.2 ps after UV activation (266 nm) were verified as showing a transient molecular response character. A localization to the terminated phenyl substructure in the complex structural environment on the surface was ensured according to the core-level nature of the recorded I4d. The activation of the bi-phenyl achieved by UV irradiation, corresponding to the UV absorption band-edge, was verified as being dominated by a Bπ → Bπ* phenyl excitation. Time-Dependent Density Functional Theory (TD-DFT) modellings were therefore performed. They were matched to Configuration Interaction semi-empirical calculations (CI-MNDO) verifying the Rustagi-Ducuing relation. The simulated singlet-singlet excitation spectrum was referenced to the spectra of an iodine terminated monomer and a linear oligophenyl chain (N = 8). Thus the deactivation response studied was assigned to a conical intersection promoted ? reaction pathway.

  19. Measurement of Integrated Stokes Parameters for He 3 3p State Excited by Spin-Polarized Electrons

    Institute of Scientific and Technical Information of China (English)

    DING Hai-Bing; PANG Wen-Ning; LIU Yi-Bao; SHANG Ren-Cheng

    2005-01-01

    @@ Integrated Stokes parameters Pi (i = 1, 2, 3) for the He 3 3p → 2 3S1 (388.9nm) transition after excitation from the ground state to the 3 3 P state by a transversely spin-polarized electron beam are measured in near threshold energy region. The experimental results are presented. The linear-polarization P2 are consistent with zero over the incident energy range, providing evidence for the LS coupling mechanism of the 3 3P state. The measured circular polarization P3 are non-zero, indicating strong electron-electron exchange effects in the spin-polarized electron-atom collision process.

  20. Repression of Toll-like receptor-4 by microRNA-149-3p is associated with smoking-related COPD

    Directory of Open Access Journals (Sweden)

    Shen W

    2017-02-01

    Full Text Available Wen Shen,1,* Jia Liu,2,* Guohou Zhao,1 Minjuan Fan,1 Gao Song,3 Yang Zhang,1 Zhiying Weng,3 You Zhang4 1Department of Respiratory Medicine, 2Department of Experimental Zoology, The Second Affiliated Hospital of Kunming Medical University, 3School of Pharmaceutical Science, Kunming Medical University, 4Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, People’s Republic of China *These authors contributed equally to this work Background: Smoking is the leading cause of COPD. Exploring molecular markers and understanding the pathogenic mechanisms of smoking-related COPD are helpful for early clinical diagnosis and treatment of the disease. This study aims to identify specific circulating microRNAs (miRNAs from the blood of COPD patients with a long history of smoking.Methods: Blood samples from four different groups were collected, and miRNA microarray was performed. Differential expression of miRNAs was verified by quantitative polymerase chain reaction. In vitro, THP-1 cells were cultured and stimulated with cigarette smoke extract (CSE or transfected with miR-149-3p inhibitor/mimics. Protein levels of Toll-like receptor 4 (TLR-4 and nuclear factor κB (NF-κB were detected using Western blot and immunofluorescence. Interleukin (IL-1β and tumor necrosis factor (TNF-α levels were determined by an enzyme-linked immunosorbent assay.Results: miRNA profiling revealed that the expression of 56 miRNAs was changed between the four groups. Expression of miR-149-3p in group C (non-smoker non-COPD was higher than in group S (smoker non-COPD, S-COPD (smoker with stable COPD and AE-COPD (smoker with acute exacerbation COPD. CSE stimulation down-regulated the expression of miR-149-3p and up-regulated the TLR-4 and NF-κB levels in THP-1 cells. Transfecting miR-149-3p inhibitors in THP-1 cells also increased the expression of its target genes. Furthermore, overexpression of miR-149-3p inhibited

  1. Theoretical investigations of α,α,α-trifluoro-3, -p and o-nitrotoluene by means of density functional theory.

    Science.gov (United States)

    Yildirim, G; Senol, S D; Dogruer, M; Ozturk, O; Senol, A; Tasci, A T; Terzioglu, C

    2012-01-01

    This study reports the optimized molecular structures, vibrational frequencies including Infrared intensities and Raman activities, corresponding vibrational assignments, (1)H and (13)C NMR chemical shifts, the magnitudes of the JCH and JCC coupling constants, Ultraviolet-visible (UV-vis) spectra, thermodynamic properties and atomic charges of the title compounds, α,α,α-trifluoro-3, -p and o-nitrotoluene, in the ground state by means of the density functional theory (DFT) with the standard B3LYP/6-311++G(d,p) method and basis set combination for the first time. Theoretical vibrational spectra were interpreted by normal coordinate analysis based on scaled density functional force field. The results show that the vibrational frequencies and chemical shifts calculated were obtained to be in good agreement with the experimental data. Based on the comparison between experimental results and theoretical data, the calculation level chosen is powerful approach for understanding the identification of all the molecules studied. In addition, not only were frontier molecular orbitals (HOMO and LUMO), molecular electrostatic potential (MEP) and electrostatic potential (ESP) simulated but also the dipole moment, softness, electronegativity, chemical hardness, electrophilicity index, transition state and energy band gap values were predicted. According to the investigations, all compounds were found to be useful to bond metallically and interact intermolecularly; however, the thermodynamic properties confirm that the α,α,α-trifluoro-p-nitrotoluene was more reactive and more polar than the others.

  2. 多通道反应O(3P)+CH2F的理论研究%Vibrational Mode Analysis for the Multi-channel Reactions of O(3P) with CH2F

    Institute of Scientific and Technical Information of China (English)

    曹小龙; 郭丽

    2004-01-01

    用密度泛函理论研究了氧原子与氟代甲基自由基的反应.反应中出现的所有物种的平衡构型用B3LYP方法在6-311++G(2 d,2p)基组水平上进行了优化,同时对各物种进行了频率分析;在同一理论水平上计算了各反应通道的势能面变化,分析了反应物、中间体、过渡态、产物的振动模式随反应途径的变化关系,阐明了该多通道反应的反应机理.%All species involved in the multi-channel reaction of an oxygen atom with a fluorinated methyl radical have been investigated using density functional theory (DFT) . The geometries of the reactants, intermediates, transition states and products are optimized at the B3LYP/6-311 + + G(2 d, 2p) level. The potential energy surface for this reaction is calculated at the same level of theory. Various possible production channels involved in the reaction of O(3 P) +CH2F are examined. The vibrational mode analysis is used to elucidate the relationship between the transition states, intermediates and the products. The extensive investigation shows that the reaction mechanism is reliable.

  3. Functional expression of pig renal organic anion transporter 3 (pOAT3).

    Science.gov (United States)

    Hagos, Yohannes; Braun, Isabella M; Krick, Wolfgang; Burckhardt, Gerhard; Bahn, Andrew

    2005-05-01

    With the cloning of pig renal organic anion transporter 1 (pOAT1) (Biochimie 84 (2002) 1219) we set up a model system for comparative studies of cloned and natively isolated membrane located transport proteins. Meanwhile, another transport protein involved in p-aminohippurate (PAH) uptake on the basolateral side of the proximal tubule cells was identified, designated organic anion transporter 3 (OAT3). To explore the contribution of pOAT1 to the PAH clearance in comparison to OAT3, it was the aim of this study to extend our model by cloning of the pig ortholog of OAT3. Sequence comparisons of human organic anion transporter 3 (hOAT3) with the expressed sequence tag (EST) database revealed a clone and partial sequence of the pig renal organic anion transporter 3 (pOAT3) ortholog. Sequencing of the entire open reading frame resulted in a protein of 543 amino acid residues encoded by 1632 base pairs (EMBL Acc. No. AJ587003). It showed high homologies of 81%, 80%, 76%, and 77% to the human, rabbit, rat, and mouse OAT3, respectively. A functional characterization of pOAT3 in Xenopus laevis oocytes yielded an apparent Km (Kt) for [3H]estrone sulfate of 7.8 +/- 1.3 microM. Moreover, pOAT3 mediated [3H]estrone sulfate uptake was almost abolished by 0.5 mM of glutarate, dehydroepiandosterone sulfate, or probenecid consistent with the hallmarks of OAT3 function.

  4. Precision frequency measurement of 1S0-3P1 intercombination lines of Sr isotopes

    Science.gov (United States)

    Liu, Hui; Gao, Feng; Ye-Bing, Wang; Xiao, Tian; Jie, Ren; Ben-Quan, Lu; Qin-Fang, Xu; Yu-Lin, Xie; Hong, Chang

    2015-01-01

    We report on frequency measurement of the intercombination (5s2)1S0-(5s5p)3P1 transition of the four natural isotopes of strontium, including 88Sr (82.58%), 87Sr (7.0%), 86Sr (9.86%), and 84Sr (0.56%). A narrow-linewidth laser that is locked to an ultra-low expansion (ULE) optical cavity with a finesse of 12000 is evaluated at a linewidth of 200 Hz with a fractional frequency drift of 2.8×10-13 at an integration time of 1 s. The fluorescence collector and detector are specially designed, based on a thermal atomic beam. Using a double-pass acousto-optic modulator (AOM) combined with a fiber and laser power stabilization configuration to detune the laser frequency enables high signal-to-noise ratios and precision saturated spectra to be obtained for the six transition lines, which allows us to determine the transition frequency precisely. The optical frequency is measured using an optical frequency synthesizer referenced to an H maser. Both the statistical values and the final values, including the corrections and uncertainties, are derived for a comparison with the values given in other works. Project supported by the National Natural Science Foundation of China (Grant No. 61127901) and the Key Project of the Chinese Academy of Sciences (Grant No. KJZD-EW-W02).

  5. Join the CERN ISEF special award winners | 16 June - 3 p.m.

    CERN Multimedia

    2016-01-01

    Come and join the CERN ISEF special award winners at their lightning talks session on 16 June at 3.00 p.m. in the main auditorium.   The 2016 Intel ISEF CERN special award winners on stage with the selection committee on 17 May 2016 in Phoenix, Arizona, USA. (Picture: Society for Science and the Public) Between 11 and 17 June 2016, the ten finalists of the Intel International Science and Engineering Fair (ISEF) who won the CERN Special Award, will visit CERN to partake in various educational lectures. ISEF is the world's largest international pre-college science competition, with approximately 1,700 high school students from more than 75 countries taking part. They will present their projects in short 5 minutes lightning talks' sessions at the main auditorium on Thursday 16 June at 3 p.m. The award winners would be also very happy to have a chance to interact and discuss with you af...

  6. Theoretical investigation of hyperthermal reactions at the gas-liquid interface: O (3P) and squalane.

    Science.gov (United States)

    Kim, Dongwook; Schatz, George C

    2007-06-14

    Hyperthermal collisions (5 eV) of ground-state atomic oxygen [O ((3)P)] with a liquid-saturated hydrocarbon, squalane (C(30)H(62)), have been studied using QM/MM hybrid "on-the-fly" direct dynamics. The surface structure of the liquid squalane is obtained from a classical molecular dynamics simulation using the OPLS-AA force field. The MSINDO semiempirical Hamiltonian is combined with OPLS-AA for the QM/MM calculations. In order to achieve a more consistent and efficient simulation of the collisions, we implemented a dynamic partitioning of the QM and MM atoms in which atoms are assigned to QM or MM regions based on their proximity to "seed" (open-shell) atoms that determine where bond making/breaking can occur. In addition, the number of seed atoms is allowed to increase or decrease as time evolves so that multiple reactive events can be described. The results show that H abstraction is the most important process for all incident angles, with H elimination, double H abstraction, and C-C bond cleavage also being important. A number of properties of these reactive channels, as well as inelastic nonreactive scattering, are investigated, including angular and translational energy distributions, the effect of incident collision angle, variation with depth of the reactive event within the liquid, with the reaction site on the hydrocarbon, and the effect of dynamics before and after reaction (direct reaction versus trapping reaction-desorption).

  7. Dynamics of interfacial reactions between O(3 P) atoms and long-chain liquid hydrocarbons

    Science.gov (United States)

    Allan, Mhairi; Bagot, Paul A. J.; Köhler, Sven P. K.; Reed, Stewart K.; Westacott, Robin E.; Costen, Matthew L.; McKendrick, Kenneth G.

    2007-09-01

    Recent progress that has been made towards understanding the dynamics of collisions at the gas-liquid interface is summarized briefly. We describe in this context a promising new approach to the experimental study of gas-liquid interfacial reactions that we have introduced. This is based on laser-photolytic production of reactive gas-phase atoms above the liquid surface and laser-spectroscopic probing of the resulting nascent products. This technique is illustrated for reaction of O(3P) atoms at the surface of the long-chain liquid hydrocarbon squalane (2,6,10,15,19,23-hexamethyltetracosane). Laser-induced fluorescence detection of the nascent OH has revealed mechanistically diagnostic correlations between its internal and translational energy distributions. Vibrationally excited OH molecules are able to escape the surface. At least two contributions to the product rotational distributions are identified, confirming and extending previous hypotheses of the participation of both direct and trapping-desorption mechanisms. We speculate briefly on future experimental and theoretical developments that might be necessary to address the many currently unanswered mechanistic questions for this, and other, classes of gas-liquid interfacial reaction.

  8. Prevalence of Serologic Hepatitis B Markers in Blood Donors From Puebla, Mexico: The Association of Relatively High Levels of Anti-Core Antibodies With the Detection of Surface Antigen and Genomic DNA

    Directory of Open Access Journals (Sweden)

    Sosa-Jurado

    2016-06-01

    Full Text Available Background The hepatitis B virus (HBV causes chronic hepatitis, hepatic cirrhosis, and hepatocellular carcinoma. Surface antigen (HBsAg detection is a definitive test that can confirm HBV infection, while the presence of antibodies against the core protein (anti-HBc suggests either a previous or ongoing infection or occult hepatitis B infection (OBI. Objectives The aim of the present study was to determine the prevalence of anti-HBc and HBsAg in blood donors. Further, the study aimed to estimate the anti-HBc level at which HBV DNA is detected in putative OBI cases, as well as to search for mutations in the “a” determinant associated with the non-detection of HBsAg in serum. Patients and Methods We conducted a cross-sectional study from 2003–2009. The study included 120,552 blood donors from the state of Puebla, Mexico. Different commercial systems based on microparticles (enzymatic (MEIA or chemiluminescent (CMIA were used to determine the HBsAg and anti-HBc levels. For the detection of HBV DNA, a nested polymerase chain reaction (nested PCR was used and the genotypes were determined using Sanger sequencing. Results Of the 120,552 blood donors, 1437 (1.19%, 95% CI: 1.12 - 1.26 were reactive to anti-HBc, while 82 (0.066%, 95% CI: 0.053 - 0.079 were reactive to HBsAg. Some 156 plasma samples collected in 2009 from anti-HBc-positive/HBsAg-negative blood donors were submitted for HBV DNA detection in a search for probable OBI. Viral DNA was detected in 27/156 (17.3%, 95% CI: 11.5 - 23.1. Our results show an association between HBV DNA or HBsAg and anti-HBc S/CO levels ≥ 4.0. All DNA samples were identified as genotype H and some “a” determinant mutations were identified, although none corresponded to mutations previously reported to hinder the detection of HBsAg by commercial immunoassays. Conclusions We observed that as the anti-HBc levels increase, there is a higher prevalence of the viral protein HBsAg in blood donors. Samples testing

  9. The human core exosome interacts with differentially localized processive RNases

    DEFF Research Database (Denmark)

    Tomecki, Rafal; Kristiansen, Maiken Søndergaard; Lykke-Andersen, Søren

    2010-01-01

    The eukaryotic RNA exosome is a ribonucleolytic complex involved in RNA processing and turnover. It consists of a nine-subunit catalytically inert core that serves a structural function and participates in substrate recognition. Best defined in Saccharomyces cerevisiae, enzymatic activity comes...... from the associated subunits Dis3p (Rrp44p) and Rrp6p. The former is a nuclear and cytoplasmic RNase II/R-like enzyme, which possesses both processive exo- and endonuclease activities, whereas the latter is a distributive RNase D-like nuclear exonuclease. Although the exosome core is highly conserved......, identity and arrangements of its catalytic subunits in different vertebrates remain elusive. Here, we demonstrate the association of two different Dis3p homologs--hDIS3 and hDIS3L--with the human exosome core. Interestingly, these factors display markedly different intracellular localizations: hDIS3...

  10. Kinetics of the benzyl + O(3P) reaction: a quantum chemical/statistical reaction rate theory study.

    Science.gov (United States)

    da Silva, Gabriel; Bozzelli, Joseph W

    2012-12-14

    The resonance stabilized benzyl radical is an important intermediate in the combustion of aromatic hydrocarbons and in polycyclic aromatic hydrocarbon (PAH) formation in flames. Despite being a free radical, benzyl is relatively stable in thermal, oxidizing environments, and is predominantly removed through bimolecular reactions with open-shell species other than O(2). In this study the reaction of benzyl with ground-state atomic oxygen, O((3)P), is examined using quantum chemistry and statistical reaction rate theory. C(7)H(7)O energy surfaces are generated at the G3SX level, and include several novel pathways. Transition state theory is used to describe elementary reaction kinetics, with canonical variational transition state theory applied for barrierless O atom association with benzyl. Apparent rate constants and branching ratios to different product sets are obtained as a function of temperature and pressure from solving the time-dependent master equation, with RRKM theory for microcanonical k(E). These simulations indicate that the benzyl + O reaction predominantly forms the phenyl radical (C(6)H(5)) plus formaldehyde (HCHO), with lesser quantities of the C(7)H(6)O products benzaldehyde, ortho-quinone methide, and para-quinone methide (+H), along with minor amounts of the formyl radical (HCO) + benzene. Addition of O((3)P) to the methylene site in benzyl produces a highly vibrationally excited C(7)H(7)O* adduct, the benzoxyl radical, which can β-scission to benzaldehyde + H and phenyl + HCHO. In order to account for the experimental observation of benzene as the major reaction product, a roaming radical mechanism is proposed that converts the nascent products phenyl and HCHO to benzene + HCO. Oxygen atom addition at the ortho and para ring sites in benzyl, which has not been previously considered, is shown to lead to the quinone methides + H; these species are less-stable isomers of benzaldehyde that are proposed as important combustion intermediates, but

  11. Contribution of Intronic miR-338-3p and Its Hosting Gene AATK to Compensatory β-Cell Mass Expansion.

    Science.gov (United States)

    Jacovetti, Cécile; Jimenez, Veronica; Ayuso, Eduard; Laybutt, Ross; Peyot, Marie-Line; Prentki, Marc; Bosch, Fatima; Regazzi, Romano

    2015-05-01

    The elucidation of the mechanisms directing β-cell mass regeneration and maintenance is of interest, because the deficit of β-cell mass contributes to diabetes onset and progression. We previously found that the level of the microRNA (miRNA) miR-338-3p is decreased in pancreatic islets from rodent models displaying insulin resistance and compensatory β-cell mass expansion, including pregnant rats, diet-induced obese mice, and db/db mice. Transfection of rat islet cells with oligonucleotides that specifically block miR-338-3p activity increased the fraction of proliferating β-cells in vitro and promoted survival under proapoptotic conditions without affecting the capacity of β-cells to release insulin in response to glucose. Here, we evaluated the role of miR-338-3p in vivo by injecting mice with an adeno-associated viral vector permitting specific sequestration of this miRNA in β-cells. We found that the adeno-associated viral construct increased the fraction of proliferating β-cells confirming the data obtained in vitro. miR-338-3p is generated from an intron of the gene coding for apoptosis-associated tyrosine kinase (AATK). Similarly to miR-338-3p, we found that AATK is down-regulated in rat and human islets and INS832/13 β-cells in the presence of the cAMP-raising agents exendin-4, estradiol, and a G-protein-coupled Receptor 30 agonist. Moreover, AATK expression is reduced in islets of insulin resistant animal models and selective silencing of AATK in INS832/13 cells by RNA interference promoted β-cell proliferation. The results point to a coordinated reduction of miR-338-3p and AATK under insulin resistance conditions and provide evidence for a cooperative action of the miRNA and its hosting gene in compensatory β-cell mass expansion.

  12. 高位层间隔震结构电梯井核心筒剪力墙处理方法研究%Processing method for a core shear wall with high-level story isolation technology

    Institute of Scientific and Technical Information of China (English)

    吴从晓; 周云; 吴从永; 邓雪松

    2011-01-01

    为了解决高位层间隔震结构中电梯井核心筒剪力墙在隔震层位置需断开的问题,提出一种高位层间隔震结构电梯井核心筒剪力墙处理方法(PMIT),介绍了其原理、构造、特点.采用ETABS软件对电梯井核心筒结构与外围结构之间布置粘滞阻尼器、弹簧、无阻尼器和弹簧的PMIT结构、悬挂法结构和框支剪力墙结构进行了分析,分析结果表明:①PMIT结构隔震层下部结构层间位移角和隔震位置电梯井剪力墙弯矩相比于框支剪力墙结构都有减小,而悬挂法隔震层下部层间位移相比于框支剪力墙结构放大了约150%,PMIT对隔震层下部结构刚度不会产生影响,避免隔震层下部结构出现薄弱层;②相比于悬挂法结构,布置粘滞阻尼器PMIT结构隔震层最外侧隔震支座拉力减小了约50%(仅水平地震作用),提高结构的抗倾覆性能.%A processing method for a core shear wall with high-level story isolation technology ( PMIT) was proposed, its principle, configuration, and characteristics were illustrated. The problem that the core shear wall needed to be broken in the isolation story could be overcome using the new method. With the use of ETABS software, the PMIT models of placing viscous dampers and elastic springs, or no damper and elastic spring in the pre-fixed gap between the core shear wall and the around upper structure, the model of the suspense method and the frame-support shear wall structure model were built, nonlinear time history analyses under frequent earthquakes were conducted. The results showed that (J) comparing with frame-support shear wall structures, the story drift of the substructure of the isolation story is reduced with PMIT, however, it increases about 150% using the suspense method; the structural seismic performance with PMIT is not influenced; (2)comparing with structures using the suspense method, the tension force of the lateral isolator in the isolation story

  13. Comodules over semiperfect corings

    CERN Document Server

    Caenepeel, S

    2011-01-01

    We discuss when the Rat functor associated to a coring satisfying the left $\\alpha$-condition is exact. We study the category of comodules over a semiperfect coring. We characterize semiperfect corings over artinian rings and over qF-rings.

  14. Coring Sample Acquisition Tool

    Science.gov (United States)

    Haddad, Nicolas E.; Murray, Saben D.; Walkemeyer, Phillip E.; Badescu, Mircea; Sherrit, Stewart; Bao, Xiaoqi; Kriechbaum, Kristopher L.; Richardson, Megan; Klein, Kerry J.

    2012-01-01

    A sample acquisition tool (SAT) has been developed that can be used autonomously to sample drill and capture rock cores. The tool is designed to accommodate core transfer using a sample tube to the IMSAH (integrated Mars sample acquisition and handling) SHEC (sample handling, encapsulation, and containerization) without ever touching the pristine core sample in the transfer process.

  15. External lipid PI3P mediates entry of eukaryotic pathogen effectors into plant and animal host cells.

    Science.gov (United States)

    Kale, Shiv D; Gu, Biao; Capelluto, Daniel G S; Dou, Daolong; Feldman, Emily; Rumore, Amanda; Arredondo, Felipe D; Hanlon, Regina; Fudal, Isabelle; Rouxel, Thierry; Lawrence, Christopher B; Shan, Weixing; Tyler, Brett M

    2010-07-23

    Pathogens of plants and animals produce effector proteins that are transferred into the cytoplasm of host cells to suppress host defenses. One type of plant pathogens, oomycetes, produces effector proteins with N-terminal RXLR and dEER motifs that enable entry into host cells. We show here that effectors of another pathogen type, fungi, contain functional variants of the RXLR motif, and that the oomycete and fungal RXLR motifs enable binding to the phospholipid, phosphatidylinositol-3-phosphate (PI3P). We find that PI3P is abundant on the outer surface of plant cell plasma membranes and, furthermore, on some animal cells. All effectors could also enter human cells, suggesting that PI3P-mediated effector entry may be very widespread in plant, animal and human pathogenesis. Entry into both plant and animal cells involves lipid raft-mediated endocytosis. Blocking PI3P binding inhibited effector entry, suggesting new therapeutic avenues.

  16. Atmospheric Methane in Ice Cores

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The reconstruction of air trapped in ice cores provides us the most direct information about atmospheric CH4 variations in the past history. Ice core records from the "Three Poles (Antarctica, Greenland and Tibetan Plateau)" reveal the detailed fluctuations of atmospheric CH4 concentration with time and are allowed to quantify the CH4 differences among latitudes. These data are indispensably in the farther study of the relationship between greenhouse gases and climatic change, and of the past changes in terrestrial CH4 emissions. Ice cores reconstruction indicates that atmospheric CH4 concentration has increased quickly since industrialization, and the present day's level of atmospheric CH4 (1800 ppbv) is unprecedented during the past Glacial-Interglacial climate cycles.

  17. Theoretical study on the two-band degenerate-gaps superconductors: Application to SrPt3P

    Science.gov (United States)

    Huang, Hai; Hou, Li-Chao; Zhao, Bin-Peng

    2016-09-01

    We study the magnetic properties of two-band degenerate-gaps superconductors with two-band isotropic Ginzburg-Landau theory. The exact solutions of upper critical field and London penetration depth are obtained, and the calculations reproduce the experimental data of the recently observed superconducting crystal SrPt3P in a broad temperature range. It directly underlies that SrPt3P is a multi-band superconductor with equal gaps in two Fermi surface sheets.

  18. Challenges Regarding IP Core Functional Reliability

    Science.gov (United States)

    Berg, Melanie D.; LaBel, Kenneth A.

    2017-01-01

    For many years, intellectual property (IP) cores have been incorporated into field programmable gate array (FPGA) and application specific integrated circuit (ASIC) design flows. However, the usage of large complex IP cores were limited within products that required a high level of reliability. This is no longer the case. IP core insertion has become mainstream including their use in highly reliable products. Due to limited visibility and control, challenges exist when using IP cores and subsequently compromise product reliability. We discuss challenges and suggest potential solutions to critical application IP insertion.

  19. Vibrational quenching of CO2(010) by collisions with O(3P) at thermal energies: a quantum-mechanical study.

    Science.gov (United States)

    de Lara-Castells, M P; Hernández, Marta I; Delgado-Barrio, G; Villarreal, P; López-Puertas, M

    2006-04-28

    The CO(2)(010)-O((3)P) vibrational energy transfer (VET) efficiency is a key input to aeronomical models of the energy budget of the upper atmospheres of Earth, Venus, and Mars. This work addresses the physical mechanisms responsible for the high efficiency of the VET process at the thermal energies existing in the terrestrial upper atmosphere (150 Kmechanical study of the process within a reduced-dimensionality approach. In this model, all the particles remain along a plane and the O((3)P) atom collides along the C(2v) symmetry axis of CO(2), which can present bending oscillations around the linear arrangement, while the stretching C-O coordinates are kept fixed at their equilibrium values. Two kinds of scattering calculations are performed on high-quality ab initio potential energy surfaces (PESs). In the first approach, the calculations are carried out separately for each one of the three PESs correlating to O((3)P). In the second approach, nonadiabatic effects induced by spin-orbit couplings (SOC) are also accounted for. The results presented here provide an explanation to some of the questions raised by the experiments and aeronomical observations. At thermal energies, nonadiabatic transitions induced by SOC play a key role in causing large VET efficiencies, the process being highly sensitive to the initial fine-structure level of oxygen. At higher energies, the two above-mentioned approaches tend to coincide towards an impulsive Landau-Teller mechanism of the vibrational to translational (V-T) energy transfer.

  20. MiR-125a-3p timely inhibits oligodendroglial maturation and is pathologically up-regulated in human multiple sclerosis

    Science.gov (United States)

    Lecca, Davide; Marangon, Davide; Coppolino, Giusy T.; Méndez, Aida Menéndez; Finardi, Annamaria; Costa, Gloria Dalla; Martinelli, Vittorio; Furlan, Roberto; Abbracchio, Maria P.

    2016-01-01

    In the mature central nervous system (CNS), oligodendrocytes provide support and insulation to axons thanks to the production of a myelin sheath. During their maturation to myelinating cells, oligodendroglial precursors (OPCs) follow a very precise differentiation program, which is finely orchestrated by transcription factors, epigenetic factors and microRNAs (miRNAs), a class of small non-coding RNAs involved in post-transcriptional regulation. Any alterations in this program can potentially contribute to dysregulated myelination, impaired remyelination and neurodegenerative conditions, as it happens in multiple sclerosis (MS). Here, we identify miR-125a-3p, a developmentally regulated miRNA, as a new actor of oligodendroglial maturation, that, in the mammalian CNS regulates the expression of myelin genes by simultaneously acting on several of its already validated targets. In cultured OPCs, over-expression of miR-125a-3p by mimic treatment impairs while its inhibition with an antago-miR stimulates oligodendroglial maturation. Moreover, we show that miR-125a-3p levels are abnormally high in the cerebrospinal fluid of MS patients bearing active demyelinating lesions, suggesting that its pathological upregulation may contribute to MS development, at least in part by blockade of OPC differentiation leading to impaired repair of demyelinated lesions. PMID:27698367

  1. MiR-525-3p enhances the migration and invasion of liver cancer cells by downregulating ZNF395.

    Directory of Open Access Journals (Sweden)

    Fei Pang

    Full Text Available Liver cancer is one of leading causes of cancer-related deaths. A deeper mechanistic understanding of liver cancer could lead to the development of more effective therapeutic strategies. In our previous work, we screened 646 miRNAs and identified 11 that regulate liver cancer cell migration. The current study shows that miR-525-3p is frequently up-regulated in liver cancer tissues, and enhanced expression of miR-525-3p can promote liver cancer cell migration and invasion. Zinc finger protein 395 (ZNF395 is the direct functional target gene for miR-525-3p, and it is frequently down-regulated in liver cancer tissues. High expression of ZNF395 can significantly inhibit while knockdown of ZNF395 expression can markedly enhance the migration and invasion of liver cancer cells, suggesting that ZNF395 suppresses metastasis in liver cancer. Down-regulation of ZNF395 can mediate miR-525-3p induced liver cancer cell migration and invasion. In conclusion, miR-525-3p promotes liver cancer cell migration and invasion by directly targeting ZNF395, and the fact that miR-525-3p and ZNF395 both play important roles in liver cancer progression makes them potential therapeutic targets.

  2. Chemical preparation, kinetics of thermal behavior and infrared studies of Pb3(P3O92.3H2O and Cd3(P3O92.14H2O

    Directory of Open Access Journals (Sweden)

    Said Belaaouad

    2013-05-01

    Full Text Available Chemical preparation, thermal behavior, kinetic and IR studies are given for the cyclotriphosphates Pb3(P3O92.3H2O and Cd3(P3O92.14H2O. The later cyclotriphosphates have never been studied except their crystallographic characterization and are stable in the conditions of temperature and pressure of our laboratory until 343K. The final products of the dehydration and calcination of Pb3(P3O92.3H2O and Cd3(P3O92.14H2O, under atmospheric pressure, are respectively their long chain polyphosphates, [Pb(PO32]∞ and β[Cd(PO32]∞. The intermediate product of the dehydration of Cd3(P3O92.14H2O, under atmospheric pressure, is its long chain polyphosphate form α, α[Cd(PO32]. [Pb(PO32]∞ and β[Cd(PO32]∞ are stable until their melting points at respectively 946K and 1153K. Two different methods, Ozawa and KAS have been selected in order to study the kinetics of thermal behavior of the cyclotriphosphates Pb3(P3O92.3H2O and Cd3(P3O92.14H2O for the first time. The kinetic and thermodynamic features of the dehydration, of the cited cyclotriphosphates, were determined and discussed on the basis of their crystalline structure. [Pb(PO32]∞, α[Cd(PO32] and β[Cd(PO32] have many applications in industry such as corrosion inhibitors.

  3. Relationship between miRNA-338-3p expression and progression and prognosis of human colorectal carcinoma

    Institute of Scientific and Technical Information of China (English)

    Sun Kai; Su Guiyuan; Deng Haijun; Dong Jingqing; Lei Shangtong; Li Guoxin

    2014-01-01

    Background miR-338-3p is a recently discovered miRNA and is involved in cell differentiation.However,few data are yet available on the aberrant expression of miR-338-3p in human colorectal carcinoma (CRC).This work aimed to investigate the relationship between miR-338-3p expression pattern and clinicopathological features of human CRC and the possible regulative mechanisms.Methods The 40 CRC,adjacent nontumorous tissues and 2 human CRC-derived cell lines (SW-480 and SW-620) were collected,respectively,and the total RNA and protein were isolated routinely.The miR-338-3p expression pattern was detected by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Northern blotting.Smoothened (SMO,possible target of miR-338-3p) mRNA and corresponding protein expression pattern were detected by semiquantitative RT-PCR and Western blotting.miR-338-3p expression patterns were compared between nontumor mucosa and CRC samples,graded by progression-related factors.Disease outcome was calculated by Kaplan-Meier survival analysis to determine whether miR-338-3p was related to disease-free survival (DFS) and overall survival (OS) of patients.Moreover,SMO 3'-UTR fragment was PCR amplified from genome DNA of human colon and inserted into a luciferase reporter plasmid.The luciferase reporter plasmid construct was then transfected into CRC cells together with pre-miR-338-3p or anti-miR-338-3p and the luciferase activity in the transfected cells was detected.Results The expression of miR-338-3p was significantly downregulated in CRCs than those in the adjacent nontumorous tissues,and the value was negatively related to advanced TNM stage and local invasion (P <0.01).Furthermore,miR338-3p value was decreased markedly in SW-620 cell line relative to SW-480 (P <0.01).Low expression of miR-338-3p was associated with unfavorable outcome in DFS but not in OS independent of clinical covariates.Moreover,RT-PCR and Western blotting analysis demonstrated that there was no

  4. Social capital and core network ties: a validation study of individual-level social capital measures and their association with extra- and intra-neighborhood ties, and self-rated health.

    Science.gov (United States)

    Moore, Spencer; Bockenholt, Ulf; Daniel, Mark; Frohlich, Katherine; Kestens, Yan; Richard, Lucie

    2011-03-01

    Research on social capital and health has assumed that measures of trust, participation, and perceived cohesion capture aspects of people's neighborhood social connections. This study uses data on the personal networks of 2707 Montreal adults in 300 different neighborhoods to examine the association of socio-demographic and social capital variables with the likelihood of having core ties, core neighborhood ties, and high self-rated health (SRH). Persons with higher household income were more likely to have core ties, but less likely to have core neighborhood ties. Persons with greater diversity in extra-neighborhood network capital were more likely to have core ties, and persons with greater diversity in intra-neighborhood network capital were more likely to have core neighborhood ties. Generalized trust, perceived neighborhood cohesion, and extra-neighborhood network diversity were shown associated with high SRH. Conventional measures of social capital may not capture network mechanisms. Findings suggest a critical appraisal of the mechanisms linking social capital and health, and the further delineation of network and psychosocial mechanisms in understanding these links.

  5. Visual Display of 5p-arm and 3p-arm miRNA Expression with a Mobile Application

    Directory of Open Access Journals (Sweden)

    Chao-Yu Pan

    2017-01-01

    Full Text Available MicroRNAs (miRNAs play important roles in human cancers. In previous studies, we have demonstrated that both 5p-arm and 3p-arm of mature miRNAs could be expressed from the same precursor and we further interrogated the 5p-arm and 3p-arm miRNA expression with a comprehensive arm feature annotation list. To assist biologists to visualize the differential 5p-arm and 3p-arm miRNA expression patterns, we utilized a user-friendly mobile App to display. The Cancer Genome Atlas (TCGA miRNA-Seq expression information. We have collected over 4,500 miRNA-Seq datasets from 15 TCGA cancer types and further processed them with the 5p-arm and 3p-arm annotation analysis pipeline. In order to be displayed with the RNA-Seq Viewer App, annotated 5p-arm and 3p-arm miRNA expression information and miRNA gene loci information were converted into SQLite tables. In this distinct application, for any given miRNA gene, 5p-arm miRNA is illustrated on the top of chromosome ideogram and 3p-arm miRNA is illustrated on the bottom of chromosome ideogram. Users can then easily interrogate the differentially 5p-arm/3p-arm expressed miRNAs with their mobile devices. This study demonstrates the feasibility and utility of RNA-Seq Viewer App in addition to mRNA-Seq data visualization.

  6. miR-29c-3p promotes senescence of human mesenchymal stem cells by targeting CNOT6 through p53-p21 and p16-pRB pathways.

    Science.gov (United States)

    Shang, Jin; Yao, Yuan; Fan, Xin; Shangguan, Lei; Li, Jie; Liu, Huan; Zhou, Yue

    2016-04-01

    Mesenchymal stem cells (MSCs) are important seed cells for tissue engineering and are promising targets for cell-based therapies. However, the replicative senescence of MSCs during in vitro culture limits their research and clinical applications. The molecular mechanisms underlying the replicative senescence of MSCs are not fully understood. Evidence suggests that miRNAs play important roles in replicative senescence. A microarray analysis found that the miR-29c-3p level was significantly increased during the MSC senescence process. In our study, we investigated the roles of miR-29c-3p in senescence of MSCs. We cultured MSCs for long periods of time, up and down-regulated the miR-29c-3p expression in MSCs, and examined the senescent phenotype changes. The over-expression of miR-29c-3p led to enhanced senescence-associated-β-galactosidase (SA-β-gal) staining, senescence associated secretory phenotype (SASP), senescence associated heterochromatic foci (SAHF), reduced proliferation ability, retarded osteogenic differentiation and corresponding changes in senescence markers, whereas the miR-29c-3p down-regulation had the opposite results. Dual-luciferase reporter assays demonstrated that CNOT6 is the target gene of miR-29c-3p. Knockdown of CNOT6 confirmed its inhibitory effects on the senescence of MSCs. In addition, Western blot results showed that both the p53-p21 and the p16-pRB pathways were activated during the miR-29c-3p-induced senescence of MSCs. In conclusion, our results demonstrate that miR-29c-3p promotes the senescence of MSCs by targeting CNOT6 through p53-p21 and p16-pRB pathways and highlight the contribution of post-transcriptional regulation to stem cell senescence.

  7. Experimental and theoretical studies of rate coefficients for the reaction O(3P)+CH3OH at high temperatures.

    Science.gov (United States)

    Lu, Chih-Wei; Chou, Shen-Long; Lee, Yuan-Pern; Xu, Shucheng; Xu, Z F; Lin, M C

    2005-06-22

    Rate coefficients of the reaction O((3)P) + CH(3)OH in the temperature range of 835-1777 K were determined using a diaphragmless shock tube. O atoms were generated by photolysis of SO(2) with a KrF excimer laser at 248 nm or an ArF excimer laser at 193 nm; their concentrations were monitored via atomic resonance absorption excited by emission from a microwave-discharged mixture of O(2) and He. The rate coefficients determined for the temperature range can be represented by the Arrhenius equation, k(T) = (2.29 +/- 0.18) x 10(-10) exp[-(4210 +/- 100)T] cm(3) molecule(-1) s(-1); unless otherwise noted, all the listed errors represent one standard deviation in fitting. Combination of these and previous data at lower temperature shows a non-Arrhenius behavior described as the three-parameter equation, k(T) = (2.74 +/- 0.07) x 10(-18)T(2.25 +/- 0.13) exp[-(1500 +/- 90)T] cm(3)molecule(-1) s(-1). Theoretical calculations at the Becke-3-Lee-Yang-Parr (B3LYP)6-311 + G(3df,2p) level locate three transition states. Based on the energies computed with coupled clusters singles, doubles (triples) [CCSD(T)]/6-311 + G(3df,2p)B3LYP6-311 + G(3df,2p), the rate coefficients predicted with canonical variational transition state theory with small curvature tunneling corrections agree satisfactorily with the experimental observations. The branching ratios of two accessible reaction channels forming OH + CH(2)OH (1a) and OH + CH(3)O (1b) are predicted to vary strongly with temperature. At 300 K, reaction (1a) dominates, whereas reaction (1b) becomes more important than reaction (1a) above 1700 K.

  8. Intersystem crossing and dynamics in O(3P) + C2H4 multichannel reaction: experiment validates theory.

    Science.gov (United States)

    Fu, Bina; Han, Yong-Chang; Bowman, Joel M; Angelucci, Luca; Balucani, Nadia; Leonori, Francesca; Casavecchia, Piergiorgio

    2012-06-19

    The O((3)P) + C(2)H(4) reaction, of importance in combustion and atmospheric chemistry, stands out as a paradigm reaction involving triplet- and singlet-state potential energy surfaces (PESs) interconnected by intersystem crossing (ISC). This reaction poses challenges for theory and experiments owing to the ruggedness and high dimensionality of these potentials, as well as the long lifetimes of the collision complexes. Primary products from five competing channels (H + CH(2)CHO, H + CH(3)CO, H(2) + CH(2)CO, CH(3) + HCO, CH(2) + CH(2)O) and branching ratios (BRs) are determined in crossed molecular beam experiments with soft electron-ionization mass-spectrometric detection at a collision energy of 8.4 kcal/mol. As some of the observed products can only be formed via ISC from triplet to singlet PESs, from the product BRs the extent of ISC is inferred. A new full-dimensional PES for the triplet state as well as spin-orbit coupling to the singlet PES are reported, and roughly half a million surface hopping trajectories are run on the coupled singlet-triplet PESs to compare with the experimental BRs and differential cross-sections. Both theory and experiment find almost equal contributions from the two PESs to the reaction, posing the question of how important is it to consider the ISC as one of the nonadiabatic effects for this and similar systems involved in combustion chemistry. Detailed comparisons at the level of angular and translational energy distributions between theory and experiment are presented for the two primary channel products, CH(3) + HCO and H + CH(2)CHO. The agreement between experimental and theoretical functions is excellent, implying that theory has reached the capability of describing complex multichannel nonadiabatic reactions.

  9. Computational study of collisions between O(3P) and NO(2Π) at temperatures relevant to the hypersonic flight regime.

    Science.gov (United States)

    Castro-Palacio, Juan Carlos; Nagy, Tibor; Bemish, Raymond J; Meuwly, Markus

    2014-10-28

    Reactions involving N and O atoms dominate the energetics of the reactive air flow around spacecraft when reentering the atmosphere in the hypersonic flight regime. For this reason, the thermal rate coefficients for reactive processes involving O((3)P) and NO((2)Π) are relevant over a wide range of temperatures. For this purpose, a potential energy surface (PES) for the ground state of the NO2 molecule is constructed based on high-level ab initio calculations. These ab initio energies are represented using the reproducible kernel Hilbert space method and Legendre polynomials. The global PES of NO2 in the ground state is constructed by smoothly connecting the surfaces of the grids of various channels around the equilibrium NO2 geometry by a distance-dependent weighting function. The rate coefficients were calculated using Monte Carlo integration. The results indicate that at high temperatures only the lowest A-symmetry PES is relevant. At the highest temperatures investigated (20,000 K), the rate coefficient for the "O1O2+N" channel becomes comparable (to within a factor of around three) to the rate coefficient of the oxygen exchange reaction. A state resolved analysis shows that the smaller the vibrational quantum number of NO in the reactants, the higher the relative translational energy required to open it and conversely with higher vibrational quantum number, less translational energy is required. This is in accordance with Polanyi's rules. However, the oxygen exchange channel (NO2+O1) is accessible at any collision energy. Finally, this work introduces an efficient computational protocol for the investigation of three-atom collisions in general.

  10. Development of a chromosomally integrated metabolite-inducible Leu3p-alpha-IPM "off-on" gene switch.

    Directory of Open Access Journals (Sweden)

    Maria Poulou

    Full Text Available BACKGROUND: Present technology uses mostly chimeric proteins as regulators and hormones or antibiotics as signals to induce spatial and temporal gene expression. METHODOLOGY/PRINCIPAL FINDINGS: Here, we show that a chromosomally integrated yeast 'Leu3p-alpha-IotaRhoMu' system constitutes a ligand-inducible regulatory "off-on" genetic switch with an extensively dynamic action area. We find that Leu3p acts as an active transcriptional repressor in the absence and as an activator in the presence of alpha-isopropylmalate (alpha-IotaRhoMu in primary fibroblasts isolated from double transgenic mouse embryos bearing ubiquitously expressing Leu3p and a Leu3p regulated GFP reporter. In the absence of the branched amino acid biosynthetic pathway in animals, metabolically stable alpha-IPM presents an EC(50 equal to 0.8837 mM and fast "OFF-ON" kinetics (t(50ON = 43 min, t(50OFF = 2.18 h, it enters the cells via passive diffusion, while it is non-toxic to mammalian cells and to fertilized mouse eggs cultured ex vivo. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that the 'Leu3p-alpha-IotaRhoMu' constitutes a simpler and safer system for inducible gene expression in biomedical applications.

  11. Prm3p is a pheromone-induced peripheral nuclear envelope protein required for yeast nuclear fusion.

    Science.gov (United States)

    Shen, Shu; Tobery, Cynthia E; Rose, Mark D

    2009-05-01

    Nuclear membrane fusion is the last step in the mating pathway of the yeast Saccharomyces cerevisiae. We adapted a bioinformatics approach to identify putative pheromone-induced membrane proteins potentially required for nuclear membrane fusion. One protein, Prm3p, was found to be required for nuclear membrane fusion; disruption of PRM3 caused a strong bilateral defect, in which nuclear congression was completed but fusion did not occur. Prm3p was localized to the nuclear envelope in pheromone-responding cells, with significant colocalization with the spindle pole body in zygotes. A previous report, using a truncated protein, claimed that Prm3p is localized to the inner nuclear envelope. Based on biochemistry, immunoelectron microscopy and live cell microscopy, we find that functional Prm3p is a peripheral membrane protein exposed on the cytoplasmic face of the outer nuclear envelope. In support of this, mutations in a putative nuclear localization sequence had no effect on full-length protein function or localization. In contrast, point mutations and deletions in the highly conserved hydrophobic carboxy-terminal domain disrupted both protein function and localization. Genetic analysis, colocalization, and biochemical experiments indicate that Prm3p interacts directly with Kar5p, suggesting that nuclear membrane fusion is mediated by a protein complex.

  12. Propofol Inhibits Neurogenesis of Rat Neural Stem Cells by Upregulating MicroRNA-141-3p.

    Science.gov (United States)

    Jiang, Qiliang; Wang, Yingwei; Shi, Xueyin

    2017-02-01

    Prolonged or high-dose exposure to anesthetics, such as propofol, can cause brain cell degeneration and subsequent long-term learning or memory deficits, particularly in the developing brain. However, the cellular and molecular mechanisms underlying the deleterious effects of propofol at certain stages of development remain unclear. In this study we found that propofol inhibited the proliferation, neuronal differentiation, and migration of neural stem cells (NSCs) while upregulating miR-141-3p. Silencing of miR-141-3p abrogated the effects of propofol on NSC neurogenesis. Propofol treatment downregulated IGF2BP2, a direct target of miR-141-3p, whereas overexpression of IGF2BP2 attenuated the effects of propofol and miR-141-3p on NSC neurogenesis. In short, propofol inhibits NSC neurogenesis through a mechanism involving the miR-141-3p/IGF2BP2 axis. Our results may provide a potential approach for preventing the neurodegenerative effects of propofol in the developing brain.

  13. Sediment Core Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Provides instrumentation and expertise for physical and geoacoustic characterization of marine sediments.DESCRIPTION: The multisensor core logger measures...

  14. Sediment Core Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — FUNCTION: Provides instrumentation and expertise for physical and geoacoustic characterization of marine sediments. DESCRIPTION: The multisensor core logger measures...

  15. Regulatory role of the sequences downstream from nodD3 P1 promoter of Rhizobium meliloti

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The 660 bp region between nodD3 P1 promoter and the following coding region of Rhizobium meliloti has been studied.This region is designated "downstream sequences".It consists of two potential open reading frames,ORF1 and ORF2.Studies on the role of the downstream sequences on the activity of nodD3 P1 with nod D3(P1)-lacZ fusion show that deletion of the sequences containing ORF2 causes the increase of the activity of the fusion; on the contrary,addition of extra copies of ORF2 markedly decreases the activity of the fusion.These results indicate that the product of ORF2 plays a negative role in the expression of nod D3.

  16. Biomimetic-Inspired Infrared Sensors from Zn3P2 Microwires: Study of Their Photoconductivity and Infrared Spectrum Properties

    Directory of Open Access Journals (Sweden)

    M. Israelowitz

    2014-01-01

    Full Text Available The fire beetle, Melanophila acuminata (Coleoptera: Buprestidae, senses infrared radiation at wavelengths of 3 and 10–25 microns via specialized protein-containing sensilla. Although the protein denatures outside of a biological system, this detection mechanism has inspired our bottom-up approach to produce single zinc phosphide microwires via vapour transport for IR sensing. The Zn3P2 microwires were immobilized and electrical contact was made by dielectrophoresis. Photoconductivity measurements have been extended to the near IR range, spanning the Zn3P2 band gaps. Purity and integrity of the Zn3P2 microwires including infrared light scattering properties were confirmed by infrared transmission microscopy. This biomimetic microwire shows promise for infrared chip development.

  17. Energetics, transition states, and intrinsic reaction coordinates for reactions associated with O(3P) processing of hydrocarbon materials

    Science.gov (United States)

    Yan, Tianying; Hase, William L.; Doubleday, Charles

    2004-05-01

    Electronic structure calculations based on multiconfiguration wave functions are used to investigate a set of archetypal reactions relevant to O(3P) processing of hydrocarbon molecules and surfaces. These include O(3P) reactions with methane and ethane to give OH plus methyl or ethyl radicals, O(3P)+ethane to give CH3O+CH3, and secondary reactions of the OH product radical with ethane and the ethyl radical. Geometry optimization is carried out with CASSCF/cc-pVTZ for all reactions, and with CASPT2/cc-pVTZ for O(3P)+methane/ethane. Single-point energy corrections are applied with CASPT2, CASPT3, and MRCI+Q with the cc-pVTZ and cc-pVQZ basis sets, and the energies extrapolated to the complete basis set limit (CBL). Where comparison of computed barriers and energies of reaction with experiment is possible, the agreement is good to excellent. The best agreement (within experimental error) is found for MRCI+Q/CBL applied to O(3P)+methane. For the other reactions, CASPT2/CBL and MRCI+Q/CBL predictions differ from experiment by 1-5 kcal/mol for 0 K enthalpies of reaction, and are within 1 kcal/mol of the best-estimate experimental range of 0 K barriers for O(3P)+ethane and OH+ethane. The accuracy of MRCI+Q/CBL is limited mainly by the quality of the active space. CASPT2/CBL barriers are consistently lower than MRCI+Q/CBL barriers with identical reference spaces.

  18. Na/K-ATPase signaling regulates collagen synthesis through microRNA-29b-3p in cardiac fibroblasts.

    Science.gov (United States)

    Drummond, Christopher A; Hill, Michael C; Shi, Huilin; Fan, Xiaoming; Xie, Jeffrey X; Haller, Steven T; Kennedy, David J; Liu, Jiang; Garrett, Michael R; Xie, Zijian; Cooper, Christopher J; Shapiro, Joseph I; Tian, Jiang

    2016-03-01

    Chronic kidney disease (CKD) is accompanied by cardiac fibrosis, hypertrophy, and dysfunction, which are commonly referred to as uremic cardiomyopathy. Our previous studies found that Na/K-ATPase ligands or 5/6th partial nephrectomy (PNx) induces cardiac fibrosis in rats and mice. The current study used in vitro and in vivo models to explore novel roles for microRNA in this mechanism of cardiac fibrosis formation. To accomplish this, we performed microRNA profiling with RT-qPCR based arrays on cardiac tissue from rats subjected to marinobufagenin (MBG) infusion or PNx. The analysis showed that a series of fibrosis-related microRNAs were dysregulated. Among the dysregulated microRNAs, microRNA (miR)-29b-3p, which directly targets mRNA of collagen, was consistently reduced in both PNx and MBG-infused animals. In vitro experiments demonstrated that treatment of primary cultures of adult rat cardiac fibroblasts with Na/K-ATPase ligands induced significant increases in the fibrosis marker, collagen protein, and mRNA expression compared with controls, whereas miR-29b-3p expression decreased >50%. Transfection of miR-29b-3p mimics into cardiac fibroblasts inhibited cardiotonic steroids-induced collagen synthesis. Moreover, a specific Na/K-ATPase signaling antagonist, pNaKtide, prevented ouabain-induced increases in collagen synthesis and decreases in miR-29b-3p expression in these cells. In conclusion, these data are the first to indicate that signaling through Na/K-ATPase regulates miRNAs and specifically, miR-29b-3p expression both in vivo and in vitro. Additionally, these data indicate that miR-29b-3p expression plays an important role in the formation of cardiac fibrosis in CKD.

  19. Lifetime measurement of the metastable 2{sup 3}P{sub 0} state in He-like uranium

    Energy Technology Data Exchange (ETDEWEB)

    Toleikis, S. [Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., MS 88R0192, Berkeley, CA 94720-8101 (United States) and Texas A and M University, College Station, TX (United States)]. E-mail: stoleikis@lbl.gov; Manil, B. [GANIL, Caen (France); Bednarz, G. [IFUJ, Cracow (Poland); Berdermann, E. [GSI, Darmstadt (Germany); Beyer, H.F. [GSI, Darmstadt (Germany); Bosch, F. [GSI, Darmstadt (Germany); Braeuning-Demian, A. [GSI, Darmstadt (Germany); Gumberidze, A. [GSI, Darmstadt (Germany); Indelicato, P. [Universite P. et M. Curie, Paris (France); Kozhuharov, C. [GSI, Darmstadt (Germany); Liesen, D. [GSI, Darmstadt (Germany); Marrus, R. [University of California, Berkeley, CA (United States); Mokler, P.H. [GSI, Darmstadt (Germany); Stachura, Z. [INP, Cracow (Poland); Stoehlker, T. [GSI, Darmstadt (Germany); Warczak, A. [IFUJ, Cracow (Poland)

    2005-07-01

    The lifetime of the 2{sup 3}P{sub 0} state in He-like uranium has been measured in a beam-foil time-of-flight experiment at the Gesellschaft fur Schwerionenforschung accelerator facility with the result {tau}(2{sup 3}P{sub 0})=58.2(9.5)ps. With the measured lifetime it is possible to derive a value of {delta}E{sub 2s}{sup Lamb}=76.3+/-20.6eV for the n=2 Lamb shift in uranium.

  20. A proof of the linearity conjecture for k-blocking sets in PG(n, p3), p prime

    CERN Document Server

    Lavrauw, Michel; Van de Voorde, Geertrui

    2012-01-01

    In this paper, we show that a small minimal k-blocking set in PG(n, q3), q = p^h, h >= 1, p prime, p >=7, intersecting every (n-k)-space in 1 (mod q) points, is linear. As a corollary, this result shows that all small minimal k-blocking sets in PG(n, p^3), p prime, p >=7, are Fp-linear, proving the linearity conjecture (see [7]) in the case PG(n, p3), p prime, p >= 7.

  1. SOX9 regulates microRNA miR-202-5p/3p expression during mouse testis differentiation

    DEFF Research Database (Denmark)

    Wainwright, Elanor N; Jorgensen, Joan S; Kim, Youngha;

    2013-01-01

    . Expression of the primary transcript of miR-202-5p/3p remained low in XY gonads in a conditional Sox9-null mouse model, suggesting that pri-miR-202 transcription is downstream of SOX9, a transcription factor that is both necessary and sufficient for male sex determination. We identified the pri-miR-202...... findings indicate that expression of the conserved gonad microRNA, miR-202-5p/3p, is downstream of the testis-determining factor SOX9, suggesting an early role in testis development....

  2. High lying energy positions of doubly (2pns) {sup 1,3}P{sup o} and (2pnd) {sup 1,3}P{sup o} excited states of the beryllium atom

    Energy Technology Data Exchange (ETDEWEB)

    Sakho, I., E-mail: aminafatima_sakho@yahoo.fr [UFR Sciences and Technologies, Department of physics, University of Ziguinchor, Ziguinchor (Senegal)

    2011-12-15

    The Screening Constant by Unit Nuclear Charge (SCUNC) method is used to study (2pns) {sup 1,3}P{sup o} and (2pnd) {sup 1,3}P{sup o} autoionizing states of the beryllium atom. Energy positions are reported up to n=20. In addition, resonance widths of the (2pns) {sup 1}P{sup o} states also presented. The current results compared very well to available theoretical and experimental literature values up to n=15. The accurate data presented in this work may be of interest for future experimental and theoretical studies in the photoabsorption spectrum of Be. - Highlights: > Accurate energy positions of (2pns) {sup 1,3}P{sup o} and (2pnd) {sup 1,3}P{sup o} (n=3-20) autoionizing states of Be atoms. > Currently results compared very well to theoretical and experimental literature values up to n=15. > Presently data may be of interest for future experimental and theoretical studies in the photoabsorption spectrum of Be.

  3. miR-199a-3p 靶基因预测及生物信息学分析%Prediction of miR-199a-3p targets gene and its bioinformatics analysis

    Institute of Scientific and Technical Information of China (English)

    谢小娟; 潘晶晶; 魏力强; 陈葳

    2016-01-01

    Objective To provide theoretical guidance for further research on the role of miR-1 99a-3p in formation and development of bladder cancer.Methods Mature sequence of miR-1 99a-3p was analyzed;target genes and transcription factors of miRNA-1 99a-3p were predicted,and the target genes were analyzed for gene ontology (GO)enrichment and Kyoto Encyclopedia of Genes and Genome (KEGG)pathway.Then TF-miRNA-mRNA network diagram was constructed.Results Sequences of miR-1 99a-3p were highly conserved in various species.In GO analysis,the target genes of miR-1 99a-3p were enriched in many biological processes,such as regulation of cellular process,regulation of macromolecule metabolic process,and regulation of biological process (P <0.01 ).In KEGG pathway,the target genes were mainly located in bacterial invasion pathway of epithelial cells,ECM-receptor interaction pathway,PI3K-Akt signaling pathway,MAPK signaling pathway,small cell lung cancer pathway,and proteoglycans pathway in the cancer (P <0.05).According to the TF-miRNA-mRNA network diagram,the important genes that might be regulated by miR-1 99a-3p were MYC,SP1,mTOR,NFκB,and NFκB1.Conclusion miR-1 99a-3p may directly target mTOR and participate in the formation and development of bladder cancer through regulating PI3K-Akt-mTOR signaling pathway.%目的:为深入研究 miR-199a-3p 在膀胱癌形成和发展中的作用提供理论依据。方法分析 miR-199a-3p 序列,预测其靶基因和转录因子,并对靶基因进行 GO 富集和 KEGG Pathway 分析;构建 TF-miR-199a-3p-靶基因网络调控图。结果miR-199a-3p 序列在多物种间具有高度保守性;GO 分析发现 miR-199a-3p 的靶基因参与细胞调节、代谢调节、细胞大分子生物合成等生物过程(P <0.01);KEGG Pathway 分析发现 miR-199a-3p 的靶基因显著富集在上皮细胞的细菌入侵通路、ECM 受体的相互作用通路、PI3K-Akt 信号通路、MAPK 信号通路、小细胞肺癌通路、癌症中的蛋白

  4. Microstructure and Wear Behaviors of In-situ Al2O3p/7075 Composites%原位Al2O3P/7075复合材料微观组织与磨损行为

    Institute of Scientific and Technical Information of China (English)

    刘慧敏; 杨树青; 许萍; 李进福

    2012-01-01

    采用原位反应近液相线铸造法制备具有不同质量分数的Al2O3P/7075复合材料,并对其进行干滑动磨损实验研究,通过OM,SEM,TEM等材料分析方法测试了材料的微观组织和磨损表面形貌.结果表明,原位Al2O3颗粒对7075铝合金的晶粒组织有明显细化效果,Al2O3P/7075复合材料的耐磨性比基体7075铝合金有明显的提高.原因是原位合成的复合材料界面结合良好,原位Al2O3颗粒在摩擦过程中起着抑制金属流动和支撑的双重作用.磨损表面形貌显示,原位Al2O3颗粒的加入,使磨损机制由黏着磨损变为磨粒磨损,从而改善了材料的耐磨性.%The Al2O3P/7075 Al composites were synthesized by in-situ reaction near-liquidus casting. The microstructure and dry sliding wear behavior of the prepared composites were analyzed using OM, SEM and TEM as well as wear friction testing. The results reveal that in-situ Al2O3 particle with average size of approximately <1μm is uniformly distributed in the matrix, which exhibits desirable refining effects on microstructure of 7075 Al alloy. The wear behavior of the composites is greatly superior to that of the matrix, which is attributed to the grain refining and formation of a compact interface of Al2O3P/7075 Al composites. In addition, in-situ Al2O3 particles exhibit coupling effects of inhibiting metal flow and bearing some load in process of friction. The wear mechanism of the matrix 7075A1 alloy is adhesive wear, while the wear surface of Al2O3p/7075 Al composites is superior to that of the matrix 7075 Al alloy. The wear mechanism of Al2O3P/7075 Al composites is abrasive wear, and wear resistance of Al2O3P/7075 Al is improved.

  5. Making an Ice Core.

    Science.gov (United States)

    Kopaska-Merkel, David C.

    1995-01-01

    Explains an activity in which students construct a simulated ice core. Materials required include only a freezer, food coloring, a bottle, and water. This hands-on exercise demonstrates how a glacier is formed, how ice cores are studied, and the nature of precision and accuracy in measurement. Suitable for grades three through eight. (Author/PVD)

  6. Ice Core Investigations

    Science.gov (United States)

    Krim, Jessica; Brody, Michael

    2008-01-01

    What can glaciers tell us about volcanoes and atmospheric conditions? How does this information relate to our understanding of climate change? Ice Core Investigations is an original and innovative activity that explores these types of questions. It brings together popular science issues such as research, climate change, ice core drilling, and air…

  7. Iowa Core Annual Report

    Science.gov (United States)

    Iowa Department of Education, 2015

    2015-01-01

    One central component of a great school system is a clear set of expectations, or standards, that educators help all students reach. In Iowa, that effort is known as the Iowa Core. The Iowa Core represents the statewide academic standards, which describe what students should know and be able to do in math, science, English language arts, and…

  8. Mercury's core evolution

    Science.gov (United States)

    Deproost, Marie-Hélène; Rivoldini, Attilio; Van Hoolst, Tim

    2016-10-01

    Remote sensing data of Mercury's surface by MESSENGER indicate that Mercury formed under reducing conditions. As a consequence, silicon is likely the main light element in the core together with a possible small fraction of sulfur. Compared to sulfur, which does almost not partition into solid iron at Mercury's core conditions and strongly decreases the melting temperature, silicon partitions almost equally well between solid and liquid iron and is not very effective at reducing the melting temperature of iron. Silicon as the major light element constituent instead of sulfur therefore implies a significantly higher core liquidus temperature and a decrease in the vigor of compositional convection generated by the release of light elements upon inner core formation.Due to the immiscibility in liquid Fe-Si-S at low pressure (below 15 GPa), the core might also not be homogeneous and consist of an inner S-poor Fe-Si core below a thinner Si-poor Fe-S layer. Here, we study the consequences of a silicon-rich core and the effect of the blanketing Fe-S layer on the thermal evolution of Mercury's core and on the generation of a magnetic field.

  9. Relativistic calculations of 3s2 1S0-3s3p 1P1 and 3s2 1S0-3s3p 3P1,2 transition probabilities in the Mg isoelectronic sequence

    Institute of Scientific and Technical Information of China (English)

    Cheng Cheng; Gao Xiang; Qing Bo; Zhang Xiao-Le; Li Jia-Ming

    2011-01-01

    Using the multi-configuration Dirac-Fock self-consistent field method and the relativistic configuration-interaction method, calculations of transition energies, oscillator strengths and rates are performed for the 3s2 1S0-3s3p 1P1 spinallowed transition, 3s2 1S0-3s3p 3P1,2 intercombination and magnetic quadrupole transition in the Mg isoelectronic sequence (Mg Ⅰ, Al Ⅱ, Si ⅢⅢ, P Ⅳ and S Ⅴ). Electron correlations are treated adequately, including intravalence electron correlations. The influence of the Breit interaction on oscillator strengths and transition energies are investigated. Quantum electrodynamics corrections are added as corrections. The calculation results are found to be in good agreement with the experimental data and other theoretical calculations.

  10. Experimental determination of LMFBR seismic equivalent core model

    Energy Technology Data Exchange (ETDEWEB)

    Fontaine, B.; Buland, P.; Fegeant, O.; Gantenbein, F. [CEA Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France)

    1995-12-31

    The main phenomena which influence an LMFBR core seismic response are the fluid structure interaction and the impacts between subassemblies. To study the core behaviour seismic tests and calculations have been performed on the core mock-up RAPSODIE in air or in water and for different excitation levels. (author). 2 refs., 6 figs.

  11. Mars' core and magnetism.

    Science.gov (United States)

    Stevenson, D J

    2001-07-12

    The detection of strongly magnetized ancient crust on Mars is one of the most surprising outcomes of recent Mars exploration, and provides important insight about the history and nature of the martian core. The iron-rich core probably formed during the hot accretion of Mars approximately 4.5 billion years ago and subsequently cooled at a rate dictated by the overlying mantle. A core dynamo operated much like Earth's current dynamo, but was probably limited in duration to several hundred million years. The early demise of the dynamo could have arisen through a change in the cooling rate of the mantle, or even a switch in convective style that led to mantle heating. Presently, Mars probably has a liquid, conductive outer core and might have a solid inner core like Earth.

  12. Optimal core acquisition and remanufacturing policies under uncertain core quality fractions

    NARCIS (Netherlands)

    Teunter, Ruud H.; Flapper, Simme Douwe P.

    2011-01-01

    Cores acquired by a remanufacturer are typically highly variable in quality. Even if the expected fractions of the various quality levels are known, then the exact fractions when acquiring cores are still uncertain. Our model incorporates this uncertainty in determining optimal acquisition decisions

  13. Absence of mutations in the coding sequence of the potential tumor suppressor 3pK in metastatic melanoma

    Directory of Open Access Journals (Sweden)

    Houben Roland

    2005-12-01

    Full Text Available Abstract Background Activation of Ras or Raf contributes to tumorigenesis of melanoma. However, constitutive Raf activation is also a characteristic of the majority of benign melanocytic nevi and high intensity signaling of either Ras or Raf was found to induce growth inhibition and senescence rather than transformation. Since the chromosome 3p kinase (3pK is a target of the Ras/Raf/Mek/Erk signaling pathway which antagonizes the function of the oncogene and anti-differentiation factor Bmi-1, 3pK may function as a tumor suppressor in tumors with constitutive Ras/Raf activation. Consequently, we tested whether inactivating 3pK mutations are present in melanoma. Methods 30 metastatic melanoma samples, which were positive for activating mutations of either BRaf or NRas, were analyzed for possible mutations in the 3pk gene. The 10 coding exons and their flanking intron sequences were amplified by PCR and direct sequencing of the PCR products was performed. Results This analysis revealed that besides the presence of some single nucleotide polymorphisms in the 3pk gene, we could not detect any possible loss of function mutation in any of these 30 metastatic melanoma samples selected for the presence of activating mutations within the Ras/Raf/Mek/Erk signaling pathway. Conclusion Hence, in melanoma with constitutively active Ras/Raf inactivating mutations within the 3pk gene do not contribute to the oncogenic phenotype of this highly malignant tumor.

  14. $\\beta$3$p$-spectroscopy and proton-$\\gamma$ width determination in the decay of $^{31}$Ar

    CERN Multimedia

    We propose to perform a detailed study of the $\\beta$-decay of the dripline nucleus $^{31}$Ar. This will allow a detailed study of the $\\beta$-delayed 3$p$-decay as well as provide important information on the resonances of $^{30}$S and $^{29}$P, in particular the ratio between the $p$- and $\\gamma$- partial widths relevant for astrophysics.

  15. The t-core of an s-core

    OpenAIRE

    Fayers, Matthew

    2010-01-01

    We consider the $t$-core of an $s$-core partition, when $s$ and $t$ are coprime positive integers. Olsson has shown that the $t$-core of an $s$-core is again an $s$-core, and we examine certain actions of the affine symmetric group on $s$-cores which preserve the $t$-core of an $s$-core. Along the way, we give a new proof of Olsson's result. We also give a new proof of a result of Vandehey, showing that there is a simultaneous $s$- and $t$-core which contains all others.

  16. Earth's inner core: Innermost inner core or hemispherical variations?

    NARCIS (Netherlands)

    Lythgoe, K. H.; Deuss, A.; Rudge, J. F.; Neufeld, J. A.

    2014-01-01

    The structure of Earth's deep inner core has important implications for core evolution, since it is thought to be related to the early stages of core formation. Previous studies have suggested that there exists an innermost inner core with distinct anisotropy relative to the rest of the inner core.

  17. Korrelasjon mellom core styrke, core stabilitet og utholdende styrke i core

    OpenAIRE

    Berg-Olsen, Andrea Marie; Fugelsøy, Eivor; Maurstad, Ann-Louise

    2010-01-01

    Formålet med studien var å se hvilke korrelasjon det er mellom core styrke, core stabilitet og utholdende styrke i core. Testingen bestod av tre hoveddeler hvor vi testet core styrke, core stabilitet og utholdende styrke i core. Innenfor core styrke og utholdende styrke i core ble tre ulike tester utført. Ved måling av core stabilitet ble det gjennomført kun en test. I core styrke ble isometrisk abdominal fleksjon, isometrisk rygg ekstensjon og isometrisk lateral fleksjon testet. Sit-ups p...

  18. Drilling history core hole DC-4

    Energy Technology Data Exchange (ETDEWEB)

    1978-12-01

    Core hole DC-4 was completed at a depth of 3998 feet in December, 1978 by Boyles Brothers Drilling Company, Spokane, Washington, under subcontract to Fenix and Scission, Inc. The hole was cored for the US Department of Energy and the Rockwell Hanford Operations' Basalt Waste Isolation Program. Fenix and Sicsson, Inc. furnished the engineering, daily supervision of the cable tool and core drilling activities, and geological core logging for DC-4. Core hole DC-4 is located on the Hanford Site about 3 miles east of the Yakima Barricade and approximately 103 feet southwest of rotary hole DC-5, which was completed to 3990 feet in February, 1978. Hanford Site coordinates reported for hole DC-4 are north 49,385.62 feet and west 85,207.63 feet, and Washington State coordinates are north 454,468.73 feet and east 2,209,990.87 feet. No elevation survey is available for hole DC-4, but it is approximately 745 feet above mean sea level based upon the survey of hole DC-5, which has a reported elevation of 745.16 feet on the top of the 3-inch flange. The purpose of core hole DC-4 was to core drill vertically through the basalt and interbed units for stratigraphic depth determination and core collection, and to provide a borehole for hydrologic testing, cross-hole seismic shear, and pressure wave velocity studies with rotary hole DC-5. Hole DC-4 was drilled through the overburden into basalt bedrock by cable tool methods (0-623 feet) and continuously cored through the final interval (623 to 3998 feet).Core recovery was 95.8 percent of the total footage cored.

  19. Evidence that intramolecular interactions are involved in masking the activation domain of transcriptional activator Leu3p.

    Science.gov (United States)

    Wang, D; Hu, Y; Zheng, F; Zhou, K; Kohlhaw, G B

    1997-08-01

    The Leu3 protein of Saccharomyces cerevisiae regulates the expression of genes involved in branched chain amino acid biosynthesis and in ammonia assimilation. It is modulated by alpha-isopropylmalate, an intermediate in leucine biosynthesis. In the presence of alpha-isopropylmalate, Leu3p is a transcriptional activator. In the absence of the signal molecule, the activation domain is masked, and Leu3p acts as a repressor. The recent discovery that Leu3p retains its regulatory properties when expressed in mammalian cells (Guo, H., and Kohlhaw, G. B. (1996) FEBS Lett. 390, 191-195) suggests that masking and unmasking of the activation domain occur without the participation of auxiliary proteins. Here we present experimental support for this notion and address the mechanism of masking. We show that modulation of Leu3p is exceedingly sensitive to mutations in the activation domain. An activation domain double mutant (D872N/D874N; designated Leu3-dd) was constructed that has the characteristics of a permanently masked activator. Using separately expressed segments containing either the DNA binding domain-middle region or the activation domain of wild type Leu3p (or Leu3-dd) in a modified yeast two-hybrid system, we provide direct evidence for alpha-isopropylmalate-dependent interaction between these segments. Finally, we use the phenotype of Leu3-dd-containing cells (slow growth in the absence of added leucine) to select for suppressor mutations that map to the middle region of Leu3-dd. The properties of nine such suppressors further support the idea that masking is an intramolecular process and suggest a means for mapping the surface involved in masking.

  20. Evaluation of electron population terms for 4p, 3p, and (2p): how do HOMO and LUMO shrink or expand depending on nuclear charges?

    Science.gov (United States)

    Nakanishi, Waro; Hayashi, Satoko; Narahara, Kenji; Yamaki, Daisuke; Hada, Masahiko

    2008-01-01

    Electron population terms are evaluated for N=Se, S, and O. Calculations are performed on HOMO and LUMO constructed by pure atomic 4p(Se), 3p(S), and 2p(O) orbitals, employing the 6-311+G(3d) and/or 6-311(++)G(3df,3pd) basis sets at the HF, MP2, and DFT (B3 LYP) levels. Se(4+), Se(2+), Se(0), and Se(2-) with the O(h) symmetry are called G(A: Se) and HSe(+), H(2)Se, and HSe(-) with the C(infinityh) or C(2v) symmetry are named G(B: Se), here [G(A+B: Se) in all]. HOMO and LUMO in G(A+B: N) (N=Se, S, and O) satisfy the conditions of the calculations for . The (4p), (3p), and (2p) values correlate well with the corresponding MO energies (epsilon(N)) for all calculation levels employed. Plots of (HOMO) and (LUMO) versus Q(N) (N=Se, S, and O) at the HF and MP2 levels are analyzed as two correlations. However, the plots at the DFT level can be analyzed as single correlation. A regression curve is assumed for the analysis. Behaviors of clarify how valence orbitals shrink or expand depending on Q(N). The applicability of is examined to establish a new method that enables us to analyze chemical shifts with the charge effect separately from others. A utility program derived from the Gaussian 03 (NMRANAL-NH03G) is applied to evaluate and examine the applicability to the NMR analysis.

  1. Core shroud corner joints

    Science.gov (United States)

    Gilmore, Charles B.; Forsyth, David R.

    2013-09-10

    A core shroud is provided, which includes a number of planar members, a number of unitary corners, and a number of subassemblies each comprising a combination of the planar members and the unitary corners. Each unitary corner comprises a unitary extrusion including a first planar portion and a second planar portion disposed perpendicularly with respect to the first planar portion. At least one of the subassemblies comprises a plurality of the unitary corners disposed side-by-side in an alternating opposing relationship. A plurality of the subassemblies can be combined to form a quarter perimeter segment of the core shroud. Four quarter perimeter segments join together to form the core shroud.

  2. Research about High Levels Martial Art Athletes’ Strength Training of Core Stability%高水平武术套路运动员核心稳定性力量训练的研究

    Institute of Scientific and Technical Information of China (English)

    陈芳; 宋雅伟; 赵琦

    2014-01-01

    竞技武术套路力量训练在原有传统力量训练基础上,增加核心稳定性力量训练方法,以达到提高武术套路运动员核心区域稳定性及控制力的目的。通过文献资料法、专家访谈法、试验法、数理统计法等研究方法,以江苏省武术套路队12名运动员为研究对象,进行为期12周的核心稳定性力量训练试验。研究结果表明:测试运动员核心区域控制力及稳定性均得到不同程度提高。%The martial art routine strength training in the original foundation of traditional strength training, core strength training methods to increase the stability of Mar-tial art athletes, in order to improve the core of regional stability and control force to. In this paper,through the re-search methods of literature,expert interview method,ex-perimental method,logical method,mathematical statistics method,take 12 martial art athletes in Jiangsu province as the research object,the stability of the core strength train-ing trial for 12 weeks. The research results show that:the control force and the stability of the core area of the ath-letes were improved at different degrees.

  3. Synthesis and Structure of Dinuclear Molybdenum Carbonyl Thiolate Compound[Et4N]2[Mo2(CO)8(SC6H4-CH3-p)2

    Institute of Scientific and Technical Information of China (English)

    潘国华; 庄伯涛; 魏强; 黄梁仁

    2000-01-01

    Reaction of Mo(CO)6 with p-CH3-C6H4SNa and Et4NCl· H2O in CH3 CN afforded a dinuclear molybdenum (0) compound [ Et4 N ] 2 [ Mo2 (CO) 8 ( SC6 H4 -CH3-p)2 ] (1). The crystal structure of 1. 2 MeCN was determined by X-ray diffraction. The crystallographic data: C42H60Mo2N4O8S2, Mr = 1005.07, triclinic, P 1,a=9.694(3), b=9.999(3), c=13.012(2)(A。), a=99.72(2), β=97.01(1), γ=97.36(3)°, V=1219.5(1)(A。)3, Z=1, Dc=1.40g/cm3, F(000)=532, μ=6.3cm-1 , Final R =0.038 and Rw =0.046 for 4097 reflections with I>3.0σ(I). The Xray structure analysis revealed that the Mo2 S2 core is planar. The geometry around each Mo atom is a distorted octahedron, both of which form an edge-sharing bioctahedron.The Mo…Mo distance is 4.0342(5)(A。),which obviously indicates the absence of Mo-Mo bond.

  4. Expression of caspase-3, p53 and Bcl-2 in generalized aggressive periodontitis

    Directory of Open Access Journals (Sweden)

    Özdemir B Handan

    2006-06-01

    Full Text Available Abstract Background Apoptosis, or programmed cell death is a form of physiological cell death. It is increased or decreased in the presence of infection, inflammation or tissue remodelling. Previous studies suggest that apoptosis is involved in the pathogenesis of inflammatory periodontal disease. The aim of the present study was to investigate the clinical features and known indicators of apoptosis (p53, Bcl-2, Caspase-3 in patients with generalized aggressive periodontitis (GAP Methods Eight patients with GAP, who had sites with probing depths (PD > 5 mm, and 10 periodontally-healthy persons were included in the study. Clinical examinations and PD were performed, and the plaque index and gingival index were recorded. Gingival tissues biopsies were obtained from active site of each patient and from healthy individuals. The expression of caspase-3, Bcl-2, and p53 was evaluated by immunohistochemistry Results There were no significant differences between GAP and control group with respect to levels of caspase-3 and p53 expression (P > 0.05. Contrary, the frequency of grade 3 expression of Bcl-2 was higher in GAP group than the control group. Conclusion The higher frequency of Bcl-2 expression in GAP group indicates and delayed apoptosis can lead to increasing resident inflammatory cells in periodontal tissues and resulting in progressive periodontal destruction.

  5. Polymeric nanoparticle-based delivery of microRNA-199a-3p inhibits proliferation and growth of osteosarcoma cells

    Directory of Open Access Journals (Sweden)

    Zhang L

    2015-04-01

    Full Text Available Linlin Zhang,1,2,* Arun K lyer,3,4,* Xiaoqian Yang,1 Eisuke Kobayashi,1 Yuqi Guo,1,2 Henry Mankin,1 Francis J Hornicek,1 Mansoor M Amiji,3 Zhenfeng Duan1 1Sarcoma Biology Laboratory, Center for Sarcoma and Connective Tissue Oncology, Massachusetts General Hospital, Boston, Massachusetts, USA; 2Department of Pathology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China; 3Department of Pharmaceutical Sciences, School of Pharmacy, Northeastern University, Boston, Massachusetts, USA; 4Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA *These authors contributed equally to this work Abstract: Our prior screening of microRNAs (miRs identified that miR-199a-3p expression is reduced in osteosarcoma cells, one of the most common types of bone tumor. miR-199a-3p exhibited functions of tumor cell growth inhibition, suggesting the potential application of miR-199a-3p as an anticancer agent. In the study reported here, we designed and developed a lipid-modified dextran-based polymeric nanoparticle platform for encapsulation of miRs, and determined the efficiency and efficacy of delivering miR-199a-3p into osteosarcoma cells. In addition, another potent miR, let-7a, which also displayed tumor suppressive ability, was selected as a candidate miR for evaluation. Fluorescence microscopy studies and real-time polymerase chain reaction results showed that dextran nanoparticles could deliver both miR-199a-3p and let-7a into osteosarcoma cell lines (KHOS and U-2OS successfully. Western blotting analysis and 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT assays demonstrated that dextran nanoparticles loaded with miRs could efficiently downregulate the expression of target proteins and effectively inhibit the growth and proliferation of osteosarcoma cells. These results demonstrate that a lipid-modified dextran

  6. iPSC Core

    Data.gov (United States)

    Federal Laboratory Consortium — The induced Pluripotent Stem Cells (iPSC) Core was created in 2011 to accelerate stem cell research in the NHLBI by providing investigators consultation, technical...

  7. Reference: -300CORE [PLACE

    Lifescience Database Archive (English)

    Full Text Available -300CORE Forde BG, Heyworth A, Pywell J, Kreis M Nucleotide sequence of a B1 hordein gene and the identifica...tion of possible upstream regulatory elements in endosperm storage protein genes fr

  8. Biospecimen Core Resource - TCGA

    Science.gov (United States)

    The Cancer Genome Atlas (TCGA) Biospecimen Core Resource centralized laboratory reviews and processes blood and tissue samples and their associated data using optimized standard operating procedures for the entire TCGA Research Network.

  9. NICHD Zebrafish Core

    Data.gov (United States)

    Federal Laboratory Consortium — The core[HTML_REMOVED]s goal is to help researchers of any expertise perform zebrafish experiments aimed at illuminating basic biology and human disease mechanisms,...

  10. Focusing on Core Business

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    China is regulating state-owned enterprises that are investing outside of their core business realms, concerned that poor investment decisions could lead to loss of state-owned assets, but some doubt the effect of the new regulation

  11. Organizing Core Tasks

    DEFF Research Database (Denmark)

    Boll, Karen

    Civil servants conduct the work which makes welfare states functions on an everyday bases: Police men police, school teachers teach, and tax inspectors inspect. Focus in this paper is on the core tasks of tax inspectors. The paper argues that their core task of securing the collection of revenue...... has remained much the same within the last 10 years. However, how the core task has been organized has changed considerable under the influence of various “organizing devices”. The paper focusses on how organizing devices such as risk assessment, output-focus, effect orientation, and treatment...... projects influence the organization of core tasks within the tax administration. The paper shows that the organizational transformations based on the use of these devices have had consequences both for the overall collection of revenue and for the employees’ feeling of “making a difference”. All in all...

  12. Delineation of the 3p14.1p13 microdeletion associated with syndromic distal limb contractures

    DEFF Research Database (Denmark)

    Thevenon, Julien; Monnier, Nicole; Callier, Patrick

    2014-01-01

    Distal limb contractures (DLC) represent a heterogeneous clinical and genetic condition. Overall, 20-25% of the DLC are caused by mutations in genes encoding the muscle contractile apparatus. Large interstitial deletions of the 3p have already been diagnosed by standard chromosomal analysis......, but not associated with a specific phenotype. We report on four patients with syndromic DLC presenting with a de novo 3p14.1p13 microdeletion. The clinical features associated multiple contractures, feeding problems, developmental delay, and intellectual disability. Facial dysmorphism was constant with low......-set posteriorly rotated ears and blepharophimosis. Review of previously reported cases with a precise mapping of the deletions, documented a 250 kb smallest region of overlap (SRO) necessary for DLC. This region contained one gene, EIF4E3, the first three exons of the FOXP1 gene, and an intronic enhancer of FOXP1...

  13. Photoassociation spectroscopy of long-range molecular states below the 2s + 3p 6Li2 asymptote

    Science.gov (United States)

    Gross, Christian; Chaudhuri, Saptarishi; Gan, Jaren; Dieckmann, Kai

    2015-05-01

    We present photoassociation spectra of high-lying vibrational states of the interatomic potentials correlating to the 2s + 3p asymptote of 6Li2 . Starting from an atomic cloud in a magneto-optical trap we first drive a free-to-bound transition into a molecular bound state using a tunable ultra-violet laser. Thereafter we ionize these long-range molecules using a 532 nm laser and detect the resulting ions with a channeltron. We determine the absolute positions of the transitions with MHz precision utilizing a frequency comb based calibration. Lithium dimers are extensively studied theoretically using various models and methods. Spectroscopic measurements are crucial to test and benchmark these methods and are available for various electronic states and inter-nuclear distances of 6Li2 molecule. Our study provides the first experimental observation of long-range states of the 2s + 3p asymptote of 6Li2 .

  14. Efficient transfer hydrogenation reaction Catalyzed by a dearomatized PN 3P ruthenium pincer complex under base-free Conditions

    KAUST Repository

    He, Lipeng

    2012-03-01

    A dearomatized complex [RuH(PN 3P)(CO)] (PN 3PN, N′-bis(di-tert-butylphosphino)-2,6-diaminopyridine) (3) was prepared by reaction of the aromatic complex [RuH(Cl)(PN 3P)(CO)] (2) with t-BuOK in THF. Further treatment of 3 with formic acid led to the formation of a rearomatized complex (4). These new complexes were fully characterized and the molecular structure of complex 4 was further confirmed by X-ray crystallography. In complex 4, a distorted square-pyramidal geometry around the ruthenium center was observed, with the CO ligand trans to the pyridinic nitrogen atom and the hydride located in the apical position. The dearomatized complex 3 displays efficient catalytic activity for hydrogen transfer of ketones in isopropanol. © 2011 Elsevier B.V. All rights reserved.

  15. 狼疮肾炎患者循环 miRNA 表达谱研究及循环 miR-130b-3p 的临床意义%Circulating microRNA expression profile and clinical significance of circulating miR-130b-3p in patients with lupus nephritis

    Institute of Scientific and Technical Information of China (English)

    王万鹏; 戚超君; 范竹萍; 曹琴; 沈剑萧; 李舒; 井冉; 倪兆慧; 张敏芳; 王玲; 牟姗; 王琴; 邵兴华; 方炜; 方燕; 陆任华

    2015-01-01

    Objective To analyze the changes of circulating miRNAs expression and explore their possible significance in patients with lupus nephritis (LN)at different stages.Methods There were a total of 94 serum samples from 58 LN patients and 36 healthy controls.12 serum samples selected that were comparable in gender and age were used to analyze the expression profile of circulating miRNAs with PCR array,including serum samples (n =4 in each group)from healthy control,early stage LN patients (CKD 1-3 stage),and late stage LN patients (CKD 4-5 stage);and the other 50 LN patients were used as the validation group,including 40 cases of early LN patients,and 10 late LN patients.The Nanodrop 2000 was were determined by the Spearman rank correlation coefficient.Results The used for detecting RNA concentration.Two miRNAs,miR-130b-3p and miR-1233-3p were selected as to be validated.Correlations expressions of seven miRNAs,including miR-1233-3p (P =0.019),miR-130b-3p (P =0.021 ),miR-18a-3p (P =0.021),miR-628-3p (P =0.023),miR-1260b (P =0.030),miR-1539 (P =0.041),and miR-378e (P =0.047)were increased in early stage LN patients compared with the healthy controls.On the other hand,the expression levels of most circulating miRNAs were found decreased in late stage LN patients, and no circulating miRNAs were found up-regulated more than 2 times or increased significantly compared to those in the healthy controls or early LN patients;the tatol serum RNA was reduced in late LN patients of validation group compared to that in healthy controls (P <0.001,U =4.5 )or early LN patients (P <0.001,U =18.0);the level of miR-130b-3p was significantly higher in early stage LN patients compared to healthy controls [IQR 16.2 (8.7,42.7)vs 9.6 (4.8,17.4),P =0.008,U =405.5)],and decreased significantly in late stage LN group compared to healthy controls (U =69.0,P =0.008)or early LN patients (U =46.0,P <0.001 ).The miR-1233-3p also significantly down-regulated in late stage LN group compared to healthy

  16. Stationary and dispersive features in resonant inelastic soft X-ray scattering at the Ge 3p resonances

    Energy Technology Data Exchange (ETDEWEB)

    Glover, C.J. [Department of Electronic Materials Engineering, Australian National University, Canberra, ACT 0200 (Australia); Schmitt, T. [SLS, Paul Scherrer Institut, PSI West, CH-5232 Villigen PSI (Switzerland); Mattesini, M. [Departamento de Fisica de la Tierra, Astronomia y Astrofisica I, Universidad Complutense de Madrid, E-28040 (Spain); Adell, M.; Ilver, L.; Kanski, J. [Chalmers University of Technology, SE-412 96 Goeteborg (Sweden); Kjeldgaard, L. [MAX-lab, Lund University, P.O. Box 118, SE-22100 Lund (Sweden); Agaker, M. [Department of Physics and Materials Science, Uppsala University, PO Box 530, SE-751 21 Uppsala (Sweden); Martensson, N. [MAX-lab, Lund University, P.O. Box 118, SE-22100 Lund (Sweden); Department of Physics and Materials Science, Uppsala University, PO Box 530, SE-751 21 Uppsala (Sweden); Ahuja, R.; Nordgren, J. [Department of Physics and Materials Science, Uppsala University, PO Box 530, SE-751 21 Uppsala (Sweden); Rubensson, J.-E. [Department of Physics and Materials Science, Uppsala University, PO Box 530, SE-751 21 Uppsala (Sweden)], E-mail: jan-erik.rubensson@fysik.uu.se

    2009-07-15

    Resonant inelastic soft X-ray scattering at the 3p resonances in crystalline Ge is presented. Both stationary and dispersive features are observed in a wide energy range above as well as below the ionization limits. These observations are in agreement with theoretical predictions based on a two-step model where the initially excited electron has no influence on the emission step. Excess population of states in the conduction band is found, and discussed in terms of attosecond electron dynamics.

  17. Nonlinear Zeeman effect in photoassociation spectra of $^{40}$Ca near the $^3$P$_1$+$^1$S$_0$ asymptote

    CERN Document Server

    Tiemann, Eberhard; Pachomow, Evgenij; Riehle, Fritz; Sterr, Uwe

    2015-01-01

    We present calculations of the Zeeman effect of narrow photoassociation lines of $^{40}$Ca near the $^3$P$_1$ + $^1$S$_0$ asymptote. Using a coupled-channel model we find a nonlinear Zeeman effect that even at low fields of a few mT amounts to several kHz. With this model we analyze previous measurements and give corrected long range dispersion coefficients of the $^3\\Pi_{u}$ and $^3\\Sigma^+ _{u}$ states.

  18. A software platform to analyse the ethical issues of electronic patient privacy policy: the S3P example

    OpenAIRE

    Mizani, M A; Baykal, N

    2007-01-01

    Paper‐based privacy policies fail to resolve the new changes posed by electronic healthcare. Protecting patient privacy through electronic systems has become a serious concern and is the subject of several recent studies. The shift towards an electronic privacy policy introduces new ethical challenges that cannot be solved merely by technical measures. Structured Patient Privacy Policy (S3P) is a software tool assuming an automated electronic privacy policy in an electronic healthcare setting...

  19. miR-130b-3p Upregulation Contributes to the Development of Thyroid Adenomas Targeting CCDC6 Gene

    Science.gov (United States)

    Leone, Vincenza; Langella, Concetta; Esposito, Francesco; De Martino, Marco; Decaussin-Petrucci, Myriam; Chiappetta, Gennaro; Bianco, Antonio; Fusco, Alfredo

    2015-01-01

    We have previously studied the function of microRNAs (miRNAs) in thyroid cells using the differentiated rat thyroid PC Cl 3 cells that need thyrotropin (TSH) for their growth. The miRNA expression profile examination allowed the detection of a set of miRNAs downregulated and upregulated by TSH. Here, we first demonstrated that upregulation of miR-130b-3p occurs through a protein kinase A-cAMP-responsive element binding protein (CREB)-dependent mechanism. Then, we analyzed its expression in human thyroid follicular adenomas, where a constitutive CREB activation is frequently present. miR-130b-3p results in upregulation with a high fold-change in most thyroid follicular adenomas. Then, we identified CCDC6, coding for a protein that interacts with CREB1 leading to the transcriptional repression of CREB1 target genes, as a target of this miRNA. The targeting of CCDC6 by miR-130b-3p likely accounts for the mechanism by which its upregulation contributes to the development of thyroid adenomas increasing CREB1 activity. PMID:26835423

  20. Phase transformation and disorder effect on optical and electrical properties of Zn3P2 thin films.

    Science.gov (United States)

    El Zawawi, I K; Abdel Moez, A; Hammad, T R; Ibrahim, R S

    2012-08-01

    The phase transformation of zinc phosphide (Zn(3)P(2)) thin films was detected through isochronal annealing process. The effects on isochronal annealing on the internal structural, optical and electrical properties of deposited Zn(3)P(2) thin films have been discussed. The films were prepared by thermal evaporation under constant preparation conditions of vacuum 1.3×10(-5)Torr, substrate temperature (300K), rate of deposition (∼1nm/s) and film thickness (480nm). The annealing process was carried out under vacuum for 2h at different temperatures ranging from 373 to 623K. X-ray diffraction patterns showed that the as-deposited films and those annealed at temperatures less than 623K exhibit amorphous structure, while the films annealed at 623K showed tetragonal polycrystalline structure. The optical transmission and reflection spectra were measured at the wavelength range of 190-2500nm. The absorption coefficient spectra and the degree of disorder as measured from the absorption edge were determined. The indirect and direct optical energy band gaps were evaluated for indirect allowed and direct allowed transitions for amorphous and polycrystalline films, respectively. The refractive index n(o) increases with raising the annealing temperature which refers to more condensation in the material. The electrical resistivity for Zn(3)P(2) films decreases exponentially with raising the annealing temperature up to 623K as influenced by structure transformation and decreasing the degree of disorder in the films.

  1. The fast C(3P) + CH3OH reaction as an efficient loss process for gas-phase interstellar methanol

    CERN Document Server

    Shannon, Robin J; Loison, Jean-Christophe; Caubet, Philippe; Balucani, Nadia; Seakins, Paul W; Wakelam, Valentine; Hickson, Kevin M

    2014-01-01

    Rate constants for the C(3P) + CH3OH reaction have been measured in a continuous supersonic flow reactor over the range 50 K to 296 K. C(3P) was created by the in-situ pulsed laser photolysis of CBr4, a multiphoton process which also produced some C(1D), allowing us to investigate simultaneously the low temperature kinetics of the C(1D) + CH3OH reaction. C(1D) atoms were followed by an indirect chemiluminescent tracer method in the presence of excess CH3OH. C(3P) atoms were detected by the same chemiluminescence technique and also by direct vacuum ultra-violet laser induced fluorescence (VUV LIF). Secondary measurements of product H(2S) atom formation have been undertaken allowing absolute H atom yields to be obtained by comparison with a suitable reference reaction. In parallel, statistical calculations have been performed based on ab-initio calculations of the complexes, adducts and transition states (TSs) relevant to the title reaction. By comparison with the experimental H atom yields, the preferred react...

  2. MicroRNA 874-3p Exerts Skeletal Anabolic Effects Epigenetically during Weaning by Suppressing Hdac1 Expression*

    Science.gov (United States)

    Kushwaha, Priyanka; Khedgikar, Vikram; Sharma, Deepika; Yuen, Tony; Gautam, Jyoti; Ahmad, Naseer; Karvande, Anirudha; Mishra, Prabhat R.; Trivedi, Prabodh K.; Sun, Li; Bhadada, Sanjay K.; Zaidi, Mone; Trivedi, Ritu

    2016-01-01

    Embryonic skeletogenesis and postnatal bone development require the transfer of calcium from the mother to the offspring during pregnancy and lactation. Therefore, bone resorption in the mother becomes elevated during these periods, resulting in significant maternal skeletal loss. There follows an anabolic phase around weaning during which there is a remarkable recovery of the maternal skeleton. However, the mechanism(s) of this anabolic response remain(s) largely unknown. We identified eight differentially expressed miRNAs by array profiling, of which miR-874-3p was highly expressed at weaning, a time when bone loss was noted to recover. We report that this weaning-associated miRNA is an anabolic target. Therefore, an agomir of miR-874-3p induced osteoblast differentiation and mineralization. These actions were mediated through the inhibition of Hdac1 expression and enhanced Runx2 transcriptional activation. When injected in vivo, the agomir significantly increased osteoblastogenesis and mineralization, reversed bone loss caused by ovariectomy, and increased bone strength. We speculate that elevated miR-874-3p expression during weaning enhances bone formation and that this miRNA may become a therapeutic target for conditions of bone loss. PMID:26663087

  3. Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model.

    Directory of Open Access Journals (Sweden)

    Abraham Lin

    Full Text Available Conjugation is the main mode of horizontal gene transfer that spreads antibiotic resistance among bacteria. Strategies for inhibiting conjugation may be useful for preserving the effectiveness of antibiotics and preventing the emergence of bacterial strains with multiple resistances. Filamentous bacteriophages were first observed to inhibit conjugation several decades ago. Here we investigate the mechanism of inhibition and find that the primary effect on conjugation is occlusion of the conjugative pilus by phage particles. This interaction is mediated primarily by phage coat protein g3p, and exogenous addition of the soluble fragment of g3p inhibited conjugation at low nanomolar concentrations. Our data are quantitatively consistent with a simple model in which association between the pili and phage particles or g3p prevents transmission of an F plasmid encoding tetracycline resistance. We also observe a decrease in the donor ability of infected cells, which is quantitatively consistent with a reduction in pili elaboration. Since many antibiotic-resistance factors confer susceptibility to phage infection through expression of conjugative pili (the receptor for filamentous phage, these results suggest that phage may be a source of soluble proteins that slow the spread of antibiotic resistance genes.

  4. New high-pressure polymorph of In2S3 with defect Th3P4-type structure

    Science.gov (United States)

    Lai, Xiaojing; Zhu, Feng; Wu, Ye; Huang, Rong; Wu, Xiang; Zhang, Qian; Yang, Ke; Qin, Shan

    2014-02-01

    The high pressure behavior of β-In2S3 (I41/amd and Z=16) has been studied by in situ synchrotron radiation X-ray diffraction combined with diamond anvil cell up to 71.7 GPa. Three pressure-induced phase transitions are evidenced at ~6.6 GPa, ~11.1 GPa at room temperature and 35.6 GPa after the high-temperature annealing using a portable laser heating system. The new polymorph of In2S3 at 35.6 GPa is assigned to the denser cubic defect Th3P4 structure (I4bar3d and Z=5.333), whose unit-cell parameters are a=7.557(1) Å and V=431.6(2) Å3. The Th3P4-type phase can be stable at least up to 71.7 GPa and cannot be preserved at ambient pressure. The pressure-volume relationship is well described by the second-order Birch-Murnaghan Equation of State, which yields B0=63(3) GPa and B0‧=4 (fixed) for the β-In2S3 phase and B0=87(3) GPa and B0‧=4 (fixed) for the defect Th3P4-type phase respectively.

  5. Bovine adenovirus 3 core protein precursor pVII localizes to mitochondria, and modulates ATP synthesis, mitochondrial Ca2+ and mitochondrial membrane potential.

    Science.gov (United States)

    Anand, Sanjeev K; Gaba, Amit; Singh, Jaswant; Tikoo, Suresh K

    2014-02-01

    Viruses modulate the functions of mitochondria by translocating viral proteins to the mitochondria. Subcellular fractionation and sensitivity to proteinase K/Triton X-100 treatment of mitochondrial fractions of bovine adenovirus (BAdV)-3-infected/transfected cells suggested that core protein pVII localizes to the mitochondria and contains a functional mitochondrial localization signal. Moreover, mitochondrial localization of BAdV-3 pVII appears to help in the retention of mitochondrial Ca(2+), inducing a significant increase in the levels of ATP and maintaining the mitochondrial membrane potential (MMP) in transfected cells. In contrast, mitochondrial localization of BAdV-3 pVII has no significant effect on the levels of cytoplasmic Ca(2+) and reactive oxygen species production in the transfected cells. Consistent with these results, expression of pVII in transfected cells treated with staurosporine decreased significantly the activation of caspase-3. Our results suggested that BAdV-3 pVII localizes to mitochondria, and interferes with apoptosis by inhibiting loss of the MMP and by increasing mitochondrial Ca(2+) and ATP production.

  6. Time-resolved soft X-ray core-level photoemission spectroscopy at 880 °C using the pulsed laser and synchrotron radiation and the pulse heating current

    Science.gov (United States)

    Abukawa, T.; Yamamoto, S.; Yukawa, R.; Kanzaki, S.; Mukojima, K.; Matsuda, I.

    2017-02-01

    We developed a time-resolved photoemission spectroscopy system for tracking the temporal variation in an electronic state of a heated sample. Our pump-probe method used laser and synchrotron radiation pulses on a silicon surface that was heated by a synchronized pulse current that did not interfere with the measurements. The transient surface photovoltage effect on the Si 2p core spectra was measured from room temperature to 880 °C and was found to be consistent with the thermal carrier distributions in silicon crystals at the corresponding temperatures. This versatile technique may have applications studying molecular dynamics on high temperature surfaces such as in catalytic reactions.

  7. miR-142-3p as a biomarker of blastocyst implantation failure - A pilot study

    Science.gov (United States)

    Borges Jr., Edson; Setti, Amanda Souza; Braga, Daniela P.A.F.; Geraldo, Murilo V; Figueira, Rita de Cássia S; Iaconelli Jr, Assumpto

    2016-01-01

    Objective This study aims to find whether microRNAs (miRNAs) detected in the culture medium of embryos produced in vitro could be potential biomarkers of embryo implantation. Methods Culture media samples from 36 embryos, derived from patients undergoing intracytoplasmic sperm injection (ICSI) in a private university-affiliated IVF center, were collected between January/2015 and November/2015. Samples were collected on day three and embryo transfers were performed on day five and all embryos reached the blastocyst stage. Samples were split into groups according to the embryo implantation result: Positive-Implantation-Group (n=18) or Negative-Implantation-Group (n=18). For the first analysis, samples were pooled in three sets for each group (6-7 spent media per pool). MicroRNAs were extracted from spent media and cDNA was synthesized. C. elegans miR-39 was used as RNA spike-in to normalize the gene expression analysis. The expression of microRNAs into the spent media from the Positive-Implantation-Group was compared with those from the Negative-Implantation-Group. A set of seven miRNAs (miR-21, miR-142-3p, miR-19b, miR-92a, miR-20b, miR-125a and miR148a) selected according with the literature, was tested. To check whether miRNAs could be detected in individual samples of culture media, in a second analysis, ten more samples were tested for miR-21 and miR-142-3p. Results From the sevens tested miRNAs, a significant increased expression of miR-142-3p could be noted in the Negative-Implantation-Group (P<0.001). For other three miRNAs (miR-21, miR-19b and miR-92a) a difference in expression was observed, however it did not reach a statistical significance. In addition, when ten non-redundant samples were tested to check if miRNAs could be detected in individual samples of culture media, the highly specific amplification of mature miRNAs, including miR-142-3p, could be noted. Conclusion Our findings suggest that miR-142-3p, previously described as a tumor suppressor and

  8. Yeast mitochondrial biogenesis: a role for the PUF RNA-binding protein Puf3p in mRNA localization.

    Directory of Open Access Journals (Sweden)

    Yann Saint-Georges

    Full Text Available The asymmetric localization of mRNA plays an important role in coordinating posttranscriptional events in eukaryotic cells. We investigated the peripheral mitochondrial localization of nuclear-encoded mRNAs (MLR in various conditions in which the mRNA binding protein context and the translation efficiency were altered. We identified Puf3p, a Pumilio family RNA-binding protein, as the first trans-acting factor controlling the MLR phenomenon. This allowed the characterization of two classes of genes whose mRNAs are translated to the vicinity of mitochondria. Class I mRNAs (256 genes have a Puf3p binding motif in their 3'UTR region and many of them have their MLR properties deeply affected by PUF3 deletion. Conversely, mutations in the Puf3p binding motif alter the mitochondrial localization of BCS1 mRNA. Class II mRNAs (224 genes have no Puf3p binding site and their asymmetric localization is not affected by the absence of PUF3. In agreement with a co-translational import process, we observed that the presence of puromycin loosens the interactions between most of the MLR-mRNAs and mitochondria. Unexpectedly, cycloheximide, supposed to solidify translational complexes, turned out to destabilize a class of mRNA-mitochondria interactions. Classes I and II mRNAs, which are therefore transported to the mitochondria through different pathways, correlated with different functional modules. Indeed, Class I genes code principally for the assembly factors of respiratory chain complexes and the mitochondrial translation machinery (ribosomes and translation regulators. Class II genes encode proteins of the respiratory chain or proteins involved in metabolic pathways. Thus, MLR, which is intimately linked to translation control, and the activity of mRNA-binding proteins like Puf3p, may provide the conditions for a fine spatiotemporal control of mitochondrial protein import and mitochondrial protein complex assembly. This work therefore provides new openings

  9. Magnetic induction measurements in distribution transformer cores

    Energy Technology Data Exchange (ETDEWEB)

    Paraskevopoulos, A.A.P.; Bourkas, P.D. [National Technical Univ. of Athens, Athens (Greece). Computer Engineering, High Voltage, and Electrical Measurements Laboratory; Paparigas, D. [Schneider ELVIM Electric Transformer Factory, Athens (Greece)

    2005-07-01

    Transformers need high magnetic field rates in order to operate efficiently. In this study, magnetic induction measurements of distribution transformer cores with power levels of 630 kVA were used to assess the performance of transformers in a city in Greece. A 630 kVA transformer was used to investigate whether magnetic induction measurements of the iron core arcs of the transformer were similar to the rest of the transformer's magnetic rate. Potential load losses were also investigated. Eight cores of 4 different sizes were measured in the study. Voltage transmissions were varied in order to measure the different cores. The voltage transmitted into the coil was calculated in order to compare the transformer's magnetic field with the cores. Average values for the measured cores were then calculated. Leakages were measured using a Gauss meter. Results of the study indicated that the measured values were lower than established safety limits for transformers. It was concluded that the magnetic induction measurements of transformer cores can be used to measure the overall performance of transformers. 18 refs., 8 tabs., 5 figs.

  10. TNF-α-induced NF-κB activation upregulates microRNA-150-3p and inhibits osteogenesis of mesenchymal stem cells by targeting β-catenin.

    Science.gov (United States)

    Wang, Nan; Zhou, Zubin; Wu, Tianyi; Liu, Wei; Yin, Peipei; Pan, Chenhao; Yu, Xiaowei

    2016-03-01

    Although systemic or local inflammation, commonly featured by cytokine activation, is implicated in patients with bone loss, the underlying mechanisms are still elusive. As microRNAs (miR), a class of small non-coding RNAs involved in essential physiological processes, have been found in bone cells, we aimed to investigate the role of miR for modulating osteogenesis in inflammatory milieu using human bone marrow mesenchymal stem cells (hBM-MSCs). Induced by proinflammatory cytokine TNF-α, miR-150-3p was identified as a key player in suppressing osteogenic differentiation through downregulating β-catenin, a transcriptional co-activator promoting bone formation. TNF-α treatment increased the levels of miR-150-3p, which directly targeted the 3'-UTR of β-catenin mRNA and in turn repressed its expression. In addition, we observed that miR-150-3p expression was increased by TNF-α via IKK-dependent NF-κB signalling. There are three putative NF-κB binding sites in the promoter region of miR-150, and we identified -686 region as the major NF-κB binding site for stimulation of miR-150 expression by TNF-α. Finally, the osteogenic differentiation of hBM-MSCs was inhibited by either miR-150-3p overexpression or TNF-α treatment, which was prevented by anti-miR-150-3p oligonucleotides. Taken together, our data suggested that miR-150-3p integrated inflammation signalling and osteogenic differentiation and may contribute to the inhibition effects of inflammation on bone formation, thus expanding the pathophysiological functions of microRNAs in bone diseases.

  11. Pressure Core Characterization

    Science.gov (United States)

    Santamarina, J. C.

    2014-12-01

    Natural gas hydrates form under high fluid pressure and low temperature, and are found in permafrost, deep lakes or ocean sediments. Hydrate dissociation by depressurization and/or heating is accompanied by a multifold hydrate volume expansion and host sediments with low permeability experience massive destructuration. Proper characterization requires coring, recovery, manipulation and testing under P-T conditions within the stability field. Pressure core technology allows for the reliable characterization of hydrate bearing sediments within the stability field in order to address scientific and engineering needs, including the measurement of parameters used in hydro-thermo-mechanical analyses, and the monitoring of hydrate dissociation under controlled pressure, temperature, effective stress and chemical conditions. Inherent sampling effects remain and need to be addressed in test protocols and data interpretation. Pressure core technology has been deployed to study hydrate bearing sediments at several locations around the world. In addition to pressure core testing, a comprehensive characterization program should include sediment analysis, testing of reconstituted specimens (with and without synthetic hydrate), and in situ testing. Pressure core characterization technology can be used to study other gas-charged formations such as deep sea sediments, coal bed methane and gas shales.

  12. Inner core structure behind the PKP core phase triplication

    NARCIS (Netherlands)

    Blom, Nienke A.; Deuss, Arwen; Paulssen, Hanneke; Waszek, Lauren

    2015-01-01

    The structure of the Earth's inner core is not well known between depths of ∼100–200 km beneath the inner core boundary. This is a result of the PKP core phase triplication and the existence of strong precursors to PKP phases, which hinder the measurement of inner core compressional PKIKP waves at e

  13. Obesity-insulin targeted genes in the 3p26-25 region in human studies and LG/J and SM/J mice.

    Science.gov (United States)

    Kraja, Aldi T; Lawson, Heather A; Arnett, Donna K; Borecki, Ingrid B; Broeckel, Ulrich; de las Fuentes, Lisa; Hunt, Steven C; Province, Michael A; Cheverud, James; Rao, D C

    2012-08-01

    Identifying metabolic syndrome (MetS) genes is important for novel drug development and health care. This study extends the findings on human chromosome 3p26-25 for an identified obesity-insulin factor QTL, with an LOD score above 3. A focused association analysis comprising up to 9578 African American and Caucasian subjects from the HyperGEN Network (908 African Americans and 1025 whites), the Family Heart Study (3035 whites in time 1 and 1943 in time 2), and the Framingham Heart Study (1317 in Offspring and 1320 in Generation 3) was performed. The homologous mouse region was explored in an F(16) generation of an advanced intercross between the LG/J and SM/J inbred strains, in an experiment where 1002 animals were fed low-fat (247 males; 254 females) or high-fat (253 males; 248 females) diets. Association results in humans indicate pleiotropic effects for SNPs within or surrounding CNTN4 on obesity, lipids and blood pressure traits and for SNPs near IL5RA, TRNT1, CRBN, and LRRN1 on central obesity and blood pressure. Linkage analyses of this region in LG/J×SM/J mice identify a highly significant pleiotropic QTL peak for insulin and glucose levels, as well as response to glucose challenge. The mouse results show that insulin and glucose levels interact with high and low fat diets and differential gene expression was identified for Crbn and Arl8b. In humans, ARL8B resides ~137kbps away from BHLHE40, expression of which shows up-regulation in response to insulin treatment. This focused human genetic analysis, incorporating mouse research evidenced that 3p26-25 has important genetic contributions to MetS components. Several of the candidate genes have functions in the brain. Their interaction with MetS and the brain warrants further investigation.

  14. Obesity–insulin targeted genes in the 3p26-25 region in human studies and LG/J and SM/J mice

    Science.gov (United States)

    Kraja, Aldi T.; Lawson, Heather A.; Arnett, Donna K.; Borecki, Ingrid B.; Broeckel, Ulrich; de las Fuentes, Lisa; Hunt, Steven C.; Province, Michael A.; Cheverud, James; Rao, D.C.

    2012-01-01

    Identifying metabolic syndrome (MetS) genes is important for novel drug development and health care. This study extends the findings on human chromosome 3p26-25 for an identified obesity–insulin factor QTL, with an LOD score above 3. A focused association analysis comprising up to 9578 African American and Caucasian subjects from the HyperGEN Network (908 African Americans and 1025 whites), the Family Heart Study (3035 whites in time 1 and 1943 in time 2), and the Framingham Heart Study (1317 in Offspring and 1320 in Generation 3) was performed. The homologous mouse region was explored in an F16 generation of an advanced intercross between the LG/J and SM/J inbred strains, in an experiment where 1002 animals were fed low-fat (247 males; 254 females) or high-fat (253 males; 248 females) diets. Association results in humans indicate pleiotropic effects for SNPs within or surrounding CNTN4 on obesity, lipids and blood pressure traits and for SNPs near IL5RA, TRNT1, CRBN, and LRRN1 on central obesity and blood pressure. Linkage analyses of this region in LG/J × SM/J mice identify a highly significant pleiotropic QTL peak for insulin and glucose levels, as well as response to glucose challenge. The mouse results show that insulin and glucose levels interact with high and low fat diets and differential gene expression was identified for Crbn and Arl8b. In humans, ARL8B resides ~137 kbps away from BHLHE40, expression of which shows up-regulation in response to insulin treatment. This focused human genetic analysis, incorporating mouse research evidenced that 3p26-25 has important genetic contributions to MetS components. Several of the candidate genes have functions in the brain. Their interaction with MetS and the brain warrants further investigation. PMID:22386932

  15. MiR-100-3p and miR-877-3p regulate overproduction of IL-8 and IL-1β in mesangial cells activated by secretory IgA from IgA nephropathy patients.

    Science.gov (United States)

    Liang, Yan; Zhao, Guoqiang; Tang, Lin; Zhang, Junjun; Li, Tianfang; Liu, Zhangsuo

    2016-10-01

    IgA nephropathy (IgAN) is the most common type of primary glomerulonephritis, characterized by mesangial deposition of pathogenic IgA and the injury to mesangial cells. Our previous studies indicate that secretory IgA (SIgA) plays an important role in the pathogenesis of IgAN, and miR-16 is involved in destructive process in mesangial cells mediated by the SIgA from IgAN patients. Our current study aimed to study the role of miRNAs in the effect of SIgA from IgAN patients on mesangial cells. MicroRNA microarray and cytokines assay were performed to obtain the differential microRNAs expression profile in human renal mesangial cells stimulated by SIgA from IgAN patients (P-SIgA) with the cells treated by SIgA from healthy subjects (N-SgA) as control. The microRNAs with the most significant differences in microarray analysis were validated by quantitative RT-PCR. Among them, miR-100-3p and miR-877-3p were selected to predict target gene related to cytokines detecting in this study. Fifty-six differentially expressed microRNAs were chosen and 17 microRNAs with the most prominent changes were validated. Compared with N-SIgA, P-SIgA increased the production of interleukin (IL)-1β, IL-8, monocyte chemotactic protein-1 and transforming growth factor-β1. In addition, we for the first time demonstrated that over-production of IL-8 induced by the SIgA was regulated by down-expression of miR-100-3p in mesangial cells. Similarly, IL-1β over-production was regulated by down-expression of miR-877-3p. Our findings represent a pathogenic microRNAs expression profiling in human mesangial cells activated by P-SIgA. Furthermore, we provide a new explanation characterizing the molecular mechanism responsible for the regulation of IL-1β and IL-8 production in P-SIgA-triggered mesangial cells.

  16. Oak Ridge National Laboratory Core Competencies

    Energy Technology Data Exchange (ETDEWEB)

    Roberto, J.B.; Anderson, T.D.; Berven, B.A.; Hildebrand, S.G.; Hartman, F.C.; Honea, R.B.; Jones, J.E. Jr.; Moon, R.M. Jr.; Saltmarsh, M.J.; Shelton, R.B. [and others

    1994-12-01

    A core competency is a distinguishing integration of capabilities which enables an organization to deliver mission results. Core competencies represent the collective learning of an organization and provide the capacity to perform present and future missions. Core competencies are distinguishing characteristics which offer comparative advantage and are difficult to reproduce. They exhibit customer focus, mission relevance, and vertical integration from research through applications. They are demonstrable by metrics such as level of investment, uniqueness of facilities and expertise, and national impact. The Oak Ridge National Laboratory (ORNL) has identified four core competencies which satisfy the above criteria. Each core competency represents an annual investment of at least $100M and is characterized by an integration of Laboratory technical foundations in physical, chemical, and materials sciences; biological, environmental, and social sciences; engineering sciences; and computational sciences and informatics. The ability to integrate broad technical foundations to develop and sustain core competencies in support of national R&D goals is a distinguishing strength of the national laboratories. The ORNL core competencies are: 9 Energy Production and End-Use Technologies o Biological and Environmental Sciences and Technology o Advanced Materials Synthesis, Processing, and Characterization & Neutron-Based Science and Technology. The distinguishing characteristics of each ORNL core competency are described. In addition, written material is provided for two emerging competencies: Manufacturing Technologies and Computational Science and Advanced Computing. Distinguishing institutional competencies in the Development and Operation of National Research Facilities, R&D Integration and Partnerships, Technology Transfer, and Science Education are also described. Finally, financial data for the ORNL core competencies are summarized in the appendices.

  17. Birefringent hollow core fibers

    DEFF Research Database (Denmark)

    Roberts, John

    2007-01-01

    Hollow core photonic crystal fiber (HC-PCF), fabricated according to a nominally non-birefringent design, shows a degree of un-controlled birefringence or polarization mode dispersion far in excess of conventional non polarization maintaining fibers. This can degrade the output pulse in many...... and an increased overlap between the polarization modes at the glass interfaces. The interplay between these effects leads to a wavelength for optimum polarization maintenance, lambda(PM), which is detuned from the wavelength of highest birefringence. By a suitable fiber design involving antiresonance of the core...

  18. MiRNA-362-3p induces cell cycle arrest through targeting of E2F1, USF2 and PTPN1 and is associated with recurrence of colorectal cancer.

    Science.gov (United States)

    Christensen, Lise Lotte; Tobiasen, Heidi; Holm, Anja; Schepeler, Troels; Ostenfeld, Marie S; Thorsen, Kasper; Rasmussen, Mads H; Birkenkamp-Demtroeder, Karin; Sieber, Oliver M; Gibbs, Peter; Lubinski, Jan; Lamy, Philippe; Laurberg, Søren; Oster, Bodil; Hansen, Kristian Q; Hagemann-Madsen, Rikke; Byskov, Kristina; Ørntoft, Torben F; Andersen, Claus L

    2013-07-01

    Colorectal cancer (CRC) is one of the leading causes of cancer deaths in Western countries. A significant number of CRC patients undergoing curatively intended surgery subsequently develop recurrence and die from the disease. MicroRNAs (miRNAs) are aberrantly expressed in cancers and appear to have both diagnostic and prognostic significance. In this study, we identified novel miRNAs associated with recurrence of CRC, and their possible mechanism of action. TaqMan(®) Human MicroRNA Array Set v2.0 was used to profile the expression of 667 miRNAs in 14 normal colon mucosas and 46 microsatellite stable CRC tumors. Four miRNAs (miR-362-3p, miR-570, miR-148 a* and miR-944) were expressed at a higher level in tumors from patients with no recurrence (p<0.015), compared with tumors from patients with recurrence. A significant association with increased disease free survival was confirmed for miR-362-3p in a second independent cohort of 43 CRC patients, using single TaqMan(®) microRNA assays. In vitro functional analysis showed that over-expression of miR-362-3p in colon cancer cell lines reduced cell viability, and proliferation mainly due to cell cycle arrest. E2F1, USF2 and PTPN1 were identified as potential miR-362-3p targets by mRNA profiling of HCT116 cells over-expressing miR-362-3p. Subsequently, these genes were confirmed as direct targets by Luciferase reporter assays and their knockdown in vitro phenocopied the effects of miR-362-3p over-expression. We conclude that miR-362-3p may be a novel prognostic marker in CRC, and hypothesize that the positive effects of augmented miR-362-3p expression may in part be mediated through the targets E2F1, USF2 and PTPN1.

  19. 99Tcm-3P4-RGD2 SPECT显像对孤立性肺结节的诊断价值%Diagnostic value of solitary pulmonary nodules using 99Tcm-3P4-RGD2 scintigraphy

    Institute of Scientific and Technical Information of China (English)

    孙昱; 王任婕; 高识; 纪铁凤; 纪滨; 贾兵; 戚良晨; 马庆杰

    2013-01-01

    Objective To investigate the value of 99Tcm-HYNIC-(poly-(ethylene glycol),PEG) 4-E (PEG4-c(RGDfK))2 (99Tcm-3P4-RGD2) SPECT imaging in the diagnosis of SPN by visual and semiquantitative analysis.Methods Twenty-one patients (13 men,8 women; age range 37-77 (58±11) years)with SPN observed on CT were analyzed prospectively.All patients underwent SPECT imaging after administration of 99Tcm-3P4-RGD2 with a dose of (939 ± 118) MBq.The gold standard was based on the histopathological diagnosis of the surgical samples from all recruited patients.The diagnostic performance of CT,SPECT visual and semiquantitative analysis was compared and analyzed with ROC curves.Immunohistochemistry was performed in part of the samples to obtain the information of integrin αvβ3 expression.Informed consent was obtained from all patients.Application of a new radiopharmaceutical was permitted and approved by the local independent Ethics Committee and the Institutional Review Board of the China-Japan Union Hospital.One-way analysis of variance,two-sample t test were used with SPSS 13.0.Results Among the 21 SPN patients,15 cases (71%) were diagnosed as malignant,and the other 6 (29%) were benign.T/NT ratio in malignant SPN was higher than that in benign SPN (1.87 ± 0.39 vs 1.41 ± 0.65),but with no statistical significance (t =2.01,P > 0.05).The sensitivities for CT,SPECT visual and semiquantitative analysis were 80% (12/15),100% (15/15) and 100% (15/15) respectively,and the specificity were all 4/6.The AUC was 0.811 (95% CI 58%-95%) for CT,0.833 (95% CI 61%-96%) for SPECT and 0.844 (95% CI 62%-96%) for T/NT ratios,which showed no statistical significance (F =0.83,P >0.05).Immunohistochemistry confirmed αvβ3 expression in both the malignant and benign nodules which had uptake of 99Tcm-3P4-RGD2 in SPECT imaging.Conclusion SPECT visual and semiquantitative analysis with 99Tcm-3P4-RGD2 appears to have high sensitivity and consistency in diagnosis of SPN

  20. O(3P)+HBr(DBr)反应的含时量子散射计算%Time-dependent Quantum Scattering Calculation of the O(3P)+HBr(DBr) Reaction

    Institute of Scientific and Technical Information of China (English)

    左国平; 唐壁玉; 韩克利

    2005-01-01

    An exact three-dimensional time-dependent quantum wave packet was employed to calculate the O(3P) +HBr(DBr) reaction using a generalized London-Ering-Polanyi-Sato(LEPS) potential energy surface. The results showed that vibrational excitation is effective for the reaction, and rotational excitation has an orientational effect in definite energy range. The rate constants and the reaction cross sections for the title reactions have been computed, the calculated rate constants ko+HBr agreed well with experimental data. By comparing with relevant results, it can be found that the kinetic isotopic effects of the reaction are relatively obvious.%基于LEPS势能面,用三维含时量子波包法对O(3P)+HBr(DBr)反应进行了准确的动力学计算.计算的结果表明,振动激发对这个反应是有效的,而转动激发在某一能量范围内具有方位效应.计算得到了该反应的速率常数和反应截面,速率常数kO+HBr的计算值同实验值符合得很好.通过对相应结果的对比,可以发现这个反应具有比较明显的同位素效应.

  1. Dynamics of carbon-hydrogen and carbon-methyl exchanges in the collision of 3P atomic carbon with propene

    Science.gov (United States)

    Lee, Shih-Huang; Chen, Wei-Kan; Chin, Chih-Hao; Huang, Wen-Jian

    2013-11-01

    We investigated the dynamics of the reaction of 3P atomic carbon with propene (C3H6) at reactant collision energy 3.8 kcal mol-1 in a crossed molecular-beam apparatus using synchrotron vacuum-ultraviolet ionization. Products C4H5, C4H4, C3H3, and CH3 were observed and attributed to exit channels C4H5 + H, C4H4 + 2H, and C3H3 + CH3; their translational-energy distributions and angular distributions were derived from the measurements of product time-of-flight spectra. Following the addition of a 3P carbon atom to the C=C bond of propene, cyclic complex c-H2C(C)CHCH3 undergoes two separate stereoisomerization mechanisms to form intermediates E- and Z-H2CCCHCH3. Both the isomers of H2CCCHCH3 in turns decompose to C4H5 + H and C3H3 + CH3. A portion of C4H5 that has enough internal energy further decomposes to C4H4 + H. The three exit channels C4H5 + H, C4H4 + 2H, and C3H3 + CH3 have average translational energy releases 13.5, 3.2, and 15.2 kcal mol-1, respectively, corresponding to fractions 0.26, 0.41, and 0.26 of available energy deposited to the translational degrees of freedom. The H-loss and 2H-loss channels have nearly isotropic angular distributions with a slight preference at the forward direction particularly for the 2H-loss channel. In contrast, the CH3-loss channel has a forward and backward peaked angular distribution with an enhancement at the forward direction. Comparisons with reactions of 3P carbon atoms with ethene, vinyl fluoride, and vinyl chloride are stated.

  2. The effects of surface temperature on the gas-liquid interfacial reaction dynamics of O(3P)+squalane

    Science.gov (United States)

    Köhler, Sven P. K.; Allan, Mhairi; Kelso, Hailey; Henderson, David A.; McKendrick, Kenneth G.

    2005-01-01

    OH/OD product state distributions arising from the reaction of gas-phase O(3P) atoms at the surface of the liquid hydrocarbon squalane C30H62/C30D62 have been measured. The O(3P) atoms were generated by 355 nm laser photolysis of NO2 at a low pressure above the continually refreshed liquid. It has been shown unambiguously that the hydroxyl radicals detected by laser-induced fluorescence originate from the squalane surface. The gas-phase OH/OD rotational populations are found to be partially sensitive to the liquid temperature, but do not adapt to it completely. In addition, rotational temperatures for OH/OD(v'=1) are consistently colder (by 34±5 K) than those for OH/OD(v'=0). This is reminiscent of, but less pronounced than, a similar effect in the well-studied homogeneous gas-phase reaction of O(3P) with smaller hydrocarbons. We conclude that the rotational distributions are composed of two different components. One originates from a direct abstraction mechanism with product characteristics similar to those in the gas phase. The other is a trapping-desorption process yielding a thermal, Boltzmann-like distribution close to the surface temperature. This conclusion is consistent with that reached previously from independent measurements of OH product velocity distributions in complementary molecular-beam scattering experiments. It is further supported by the temporal profiles of OH/OD laser-induced fluorescence signals as a function of distance from the surface observed in the current experiments. The vibrational branching ratios for (v'=1)/(v'=0) for OH and OD have been found to be (0.07±0.02) and (0.30±0.10), respectively. The detection of vibrationally excited hydroxyl radicals suggests that secondary and/or tertiary hydrogen atoms may be accessible to the attacking oxygen atoms.

  3. Hepatitis B core-related antigen levels are associated with response to entecavir and peginterferon add-on therapy in hepatitis B e antigen-positive chronic hepatitis B patients.

    Science.gov (United States)

    van Campenhout, M J H; Brouwer, W P; van Oord, G W; Xie, Q; Zhang, Q; Zhang, N; Guo, S; Tabak, F; Streinu-Cercel, A; Wang, J; Pas, S D; Sonneveld, M J; de Knegt, R J; Boonstra, A; Hansen, B E; Janssen, H L A

    2016-06-01

    Hepatitis B core-related antigen (HBcrAg), a new serum marker, may be useful in monitoring chronic hepatitis B infection. HBcrAg was measured in 175 hepatitis B e antigen-positive patients treated with entecavir (ETV) with or without peginterferon (PEG-IFN) add-on therapy. Decline in HBcrAg was stronger in patients with vs. without combined response (ETV: -3.22 vs. -1.71 log U/mL, p HBcrAg was associated with combined response (adjusted odds ratio 0.3, 95% confidence interval 0.2-0.5, p <0.001), but was not superior to quantitative HBsAg (qHBsAg).

  4. 研华推出Core双核AIMB-780新型工业级母板%Advantech Putting out Core Bi-kernel AIMB-780 New-type Industry-level Motherboard

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    @@ 作为嵌入式平台和整合服务提供商的研华公司近期发布了一款新型工业级ATX母板--AIMB-780.该款母板采用LGA 1156插槽,支持最新的Inter Core i7/i5/i3/Xeon?/Pentium?处理器.AIMB-780还支持带ECC DDR3的Intrel服务器级Xeon 34xx系列处理器,并支持SATA RAID 0、1、5和10,能够提供网络密集应用的可靠存储和系统保护.

  5. 七芯光子晶体光纤中百瓦量级超连续谱的产生%Hundred-watt-level sup ercontinuum generation in seven-core photonic crystal fib er

    Institute of Scientific and Technical Information of China (English)

    谌鸿伟; 陆启生; 韦会峰; 刘通; 周旋风; 李江; 童维军; 陈子伦; 陈胜平; 侯静

    2014-01-01

    采用平均功率为141.6 W的皮秒光纤激光泵浦一段国产七芯光子晶体光纤,获得了平均功率为104.2 W、连续光谱覆盖范围从750至1700 nm以上的超连续谱输出.详细研究了超连续光谱随泵浦功率增加的演变过程,并对基于该七芯光子晶体光纤的超连续谱光源的光谱拓展和功率提升潜力进行相关的分析和讨论.该研究结果对高功率超连续谱光源的发展具有一定的参考价值.%A 104.2 W supercontinuum spanning from 750 nm to beyond 1700 nm is generated by pumping a piece of home-made seven-core photonic crystal fiber with a 141.6 W picosecond fiber laser. The evolution of the output spectrum is investigated in detail with the increase of the picosecond laser power. The feasibilities of broadening the spectral range and scaling the output power for the supercontinuum source based on the seven-core photonic crystal fiber are analyzed and discussed. The research results will make a contribution to the further development of high-power supercontinuum source.

  6. Intrinsic product polarization and branch ratio in the S(1D, 3P)+HD reaction on three electronic states

    Science.gov (United States)

    Li, Lin; Dong, Shunle

    2016-09-01

    The intrinsic product polarization and intramolecular isotope effect of the S(1D, 3P)+HD reaction have been investigated on both the lowest singlet state (1A‧) and the triplet state (3A‧ and 3A″) potential energy surfaces by using quasi-classical trajectory and quantum mechanical methods. The calculations indicate that intramolecular isotope effects are different on the three electronic states. The stereodynamics study shows that the P(θr) distributions, P(ϕr) distributions, and polarization-dependent differential cross sections (PDDCSs) (00) are sensitive to mass factor and the product angular momentum vectors are not only aligned but also oriented.

  7. The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo.

    OpenAIRE

    Brown, J. T.; Bai, X.; Johnson, A. W.

    2000-01-01

    The yeast superkiller (SKI) genes were originally identified from mutations allowing increased production of killer toxin encoded by M "killer" virus, a satellite of the dsRNA virus L-A. XRN1 (SKI1) encodes a cytoplasmic 5'-exoribonuclease responsible for the majority of cytoplasmic RNA turnover, whereas SKI2, SKI3, and SKI8 are required for normal 3'-degradation of mRNA and for repression of translation of poly(A) minus RNA. Ski2p is a putative RNA helicase, Ski3p is a tetratricopeptide repe...

  8. Attosecond time delay in the photoionization of Mn in the $3p \\rightarrow 3d$ giant resonance region

    CERN Document Server

    Dolmatov, V K; Deshmukh, P C; Manson, S T

    2014-01-01

    The dramatic effect of the $3p \\rightarrow 3d$ giant autoionization resonance on time delay of photoemission from the $3d$ and $4s$ valence subshells of the Mn atom is unraveled. Strong sensitivity of the time delay of the $4s$ photoemission to the final-state term of the ion-remainder [${\\rm Mn^{+}}(4s^{1},$$^{5}S)$ vs. ${\\rm Mn^{+}}(4s^{1},$$^{7}S)$] is discovered. The features of time delay uncovered in Mn photoionization are expected to be general properties of transition-metal atoms and ions. The "spin-polarized" random phase approximation with exchange was employed in the study.

  9. Languages for Dublin Core.

    Science.gov (United States)

    Baker, Thomas

    1998-01-01

    Focusing on languages for the Dublin Core, examines the experience of some related ways to seek semantic interoperability through simplicity: planned languages, interlingua constructs, and pidgins. Also defines the conceptual and organizational problem of maintaining a metadata standard in multiple languages. (AEF)

  10. Modeling Core Collapse Supernovae

    Science.gov (United States)

    Mezzacappa, Anthony

    2017-01-01

    Core collapse supernovae, or the death throes of massive stars, are general relativistic, neutrino-magneto-hydrodynamic events. The core collapse supernova mechanism is still not in hand, though key components have been illuminated, and the potential for multiple mechanisms for different progenitors exists. Core collapse supernovae are the single most important source of elements in the Universe, and serve other critical roles in galactic chemical and thermal evolution, the birth of neutron stars, pulsars, and stellar mass black holes, the production of a subclass of gamma-ray bursts, and as potential cosmic laboratories for fundamental nuclear and particle physics. Given this, the so called ``supernova problem'' is one of the most important unsolved problems in astrophysics. It has been fifty years since the first numerical simulations of core collapse supernovae were performed. Progress in the past decade, and especially within the past five years, has been exponential, yet much work remains. Spherically symmetric simulations over nearly four decades laid the foundation for this progress. Two-dimensional modeling that assumes axial symmetry is maturing. And three-dimensional modeling, while in its infancy, has begun in earnest. I will present some of the recent work from the ``Oak Ridge'' group, and will discuss this work in the context of the broader work by other researchers in the field. I will then point to future requirements and challenges. Connections with other experimental, observational, and theoretical efforts will be discussed, as well.

  11. Core calculations of JMTR

    Energy Technology Data Exchange (ETDEWEB)

    Nagao, Yoshiharu [Japan Atomic Energy Research Inst., Oarai, Ibaraki (Japan). Oarai Research Establishment

    1998-03-01

    In material testing reactors like the JMTR (Japan Material Testing Reactor) of 50 MW in Japan Atomic Energy Research Institute, the neutron flux and neutron energy spectra of irradiated samples show complex distributions. It is necessary to assess the neutron flux and neutron energy spectra of an irradiation field by carrying out the nuclear calculation of the core for every operation cycle. In order to advance core calculation, in the JMTR, the application of MCNP to the assessment of core reactivity and neutron flux and spectra has been investigated. In this study, in order to reduce the time for calculation and variance, the comparison of the results of the calculations by the use of K code and fixed source and the use of Weight Window were investigated. As to the calculation method, the modeling of the total JMTR core, the conditions for calculation and the adopted variance reduction technique are explained. The results of calculation are shown. Significant difference was not observed in the results of neutron flux calculations according to the difference of the modeling of fuel region in the calculations by K code and fixed source. The method of assessing the results of neutron flux calculation is described. (K.I.)

  12. Some Core Contested Concepts

    Science.gov (United States)

    Chomsky, Noam

    2015-01-01

    Core concepts of language are highly contested. In some cases this is legitimate: real empirical and conceptual issues arise. In other cases, it seems that controversies are based on misunderstanding. A number of crucial cases are reviewed, and an approach to language is outlined that appears to have strong conceptual and empirical motivation, and…

  13. The core and cosmopolitans

    DEFF Research Database (Denmark)

    Dahlander, Linus; Frederiksen, Lars

    2012-01-01

    Users often interact and help each other solve problems in communities, but few scholars have explored how these relationships provide opportunities to innovate. We analyze the extent to which people positioned within the core of a community as well as people that are cosmopolitans positioned...

  14. Schumpeter's core works revisited

    DEFF Research Database (Denmark)

    Andersen, Esben Sloth

    2012-01-01

    This paper organises Schumpeter’s core books in three groups: the programmatic duology,the evolutionaryeconomic duology,and the socioeconomic synthesis. By analysing these groups and their interconnections from the viewpoint of modern evolutionaryeconomics,the paper summarises resolved problems...

  15. Adult educators' core competences

    DEFF Research Database (Denmark)

    Wahlgren, Bjarne

    2016-01-01

    environment as well as the kind of adult education concerned (e.g. basic education, work-related education etc.). However, it seems that it is possible to identify certain competence requirements which transcend national, cultural and functional boundaries. This research note summarises these common or “core...

  16. A prognostic model of triple-negative breast cancer based on miR-27b-3p and node status.

    Directory of Open Access Journals (Sweden)

    Songjie Shen

    Full Text Available OBJECTIVE: Triple-negative breast cancer (TNBC is an aggressive but heterogeneous subtype of breast cancer. This study aimed to identify and validate a prognostic signature for TNBC patients to improve prognostic capability and to guide individualized treatment. METHODS: We retrospectively analyzed the prognostic performance of clinicopathological characteristics and miRNAs in a training set of 58 patients with invasive ductal TNBC diagnosed between 2002 and 2012. A prediction model was developed based on independent clinicopathological and miRNA covariates. The prognostic value of the model was further validated in a separate set of 41 TNBC patients diagnosed between 2007 and 2008. RESULTS: Only lymph node status was marginally significantly associated with poor prognosis of TNBC (P = 0.054, whereas other clinicopathological factors, including age, tumor size, histological grade, lymphovascular invasion, P53 status, Ki-67 index, and type of surgery, were not. The expression levels of miR-27b-3p, miR-107, and miR-103a-3p were significantly elevated in the metastatic group compared with the disease-free group (P value: 0.008, 0.005, and 0.050, respectively. The Cox proportional hazards regression analysis revealed that lymph node status and miR-27b-3p were independent predictors of poor prognosis (P value: 0.012 and 0.027, respectively. A logistic regression model was developed based on these two independent covariates, and the prognostic value of the model was subsequently confirmed in a separate validation set. The two different risk groups, which were stratified according to the model, showed significant differences in the rates of distant metastasis and breast cancer-related death not only in the training set (P value: 0.001 and 0.040, respectively but also in the validation set (P value: 0.013 and 0.012, respectively. CONCLUSION: This model based on miRNA and node status covariates may be used to stratify TNBC patients into different

  17. Identification of an amphipathic helix important for the formation of ectopic septin spirals and axial budding in yeast axial landmark protein Bud3p.

    Science.gov (United States)

    Guo, Jia; Gong, Ting; Gao, Xiang-Dong

    2011-03-08

    Correct positioning of polarity axis in response to internal or external cues is central to cellular morphogenesis and cell fate determination. In the budding yeast Saccharomyces cerevisiae, Bud3p plays a key role in the axial bud-site selection (axial budding) process in which cells assemble the new bud next to the preceding cell division site. Bud3p is thought to act as a component of a spatial landmark. However, it is not clear how Bud3p interacts with other components of the landmark, such as the septins, to control axial budding. Here, we report that overexpression of Bud3p causes the formation of small septin rings (∼1 µm in diameter) and arcs aside from previously reported spiral-like septin structures. Bud3p closely associates with the septins in vivo as Bud3p colocalizes with these aberrant septin structures and forms a complex with two septins, Cdc10p and Cdc11p. The interaction of Bud3p with the septins may involve multiple regions of Bud3p including 1-858, 850-1220, and 1221-1636 a.a. since they all target to the bud neck but exhibit different effects on septin organization when overexpressed. In addition, our study reveals that the axial budding function of Bud3p is mediated by the N-terminal region 1-858. This region shares an amphipathic helix (850-858) crucial for bud neck targeting with the middle portion 850-1103 involved in the formation of ectopic septin spirals and rings. Interestingly, the Dbl-homology domain located in 1-858 is dispensable for axial bud-site selection. Our findings suggest that multiple regions of Bud3p ensure efficient targeting of Bud3p to the bud neck in the assembly of the axial landmark and distinct domains of Bud3p are involved in axial bud-site selection and other cellular processes.

  18. MiR-338-3p targets pyruvate kinase M2 and affects cell proliferation and metabolism of ovarian cancer

    OpenAIRE

    Zhang, Yuting; Shi, Bing; Chen, Jiang; Hu, Lina; Zhao, Chunquan

    2016-01-01

    MiR-338-3p is down-regulated in cancer, which inhibits cancer cell proliferation, metastasis, and increases chemosensitivity, but its functions in ovarian cancer remains unknown. The present study aims to identify the miR-338-3p targeted genes and to investigate the associated regulatory mechanisms in ovarian cancer cell proliferation and metabolism. Our results demonstrated miR-338-3p expression was down-regulated in most of ovarian cancer tissues and cell lines. Restoration of miR-338-3p ex...

  19. miR-625-3p regulates oxaliplatin resistance by targeting MAP2K6-p38 signalling in human colorectal adenocarcinoma cells

    DEFF Research Database (Denmark)

    Rasmussen, Mads Heilskov; Lyskjær, Iben; Jersie-Christensen, Rosa Rakownikow;

    2016-01-01

    Oxaliplatin resistance in colorectal cancers (CRC) is a major medical problem, and predictive markers are urgently needed. Recently, miR-625-3p was reported as a promising predictive marker. Herein, we show that miR-625-3p functionally induces oxaliplatin resistance in CRC cells, and identify the...... as one likely mechanism of oxaliplatin resistance. Our study shows that miR-625-3p induces oxaliplatin resistance by abrogating MAP2K6-p38-regulated apoptosis and cell cycle control networks, and corroborates the predictive power of miR-625-3p....

  20. Evolution of the Internet and its cores

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Guoqing; Zhang Guoqiang; Yang Qingfeng; Cheng Suqi [Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100190 (China); Zhou Tao [Department of Modern Physics, University of Science and Technology of China, Hefei 230026 (China)], E-mail: gqzhang@ict.ac.cn, E-mail: zhutou@ustc.edu

    2008-12-15

    In this paper, we empirically study the evolution of large scale Internet topology at the autonomous system (AS) level. The network size grows in an exponential form, obeying the famous Moore's law. We theoretically predict that the size of the AS-level Internet will double every 5.32 years. We apply the k-core decomposition method on the real Internet, and find that the size of a k-core with larger k is nearly stable over time. In addition, the maximal coreness is very stable after 2003. In contrast to the predictions of most previous models, the maximal degree of the Internet is also relatively stable versus time. We use the edge-exchange operation to obtain the randomized networks with the same degree sequence. A systematical comparison is drawn, indicating that the real Internet is more loosely connected, and both the full Internet and the nucleus are more disassortative than their randomized versions.

  1. White light generation from Dy3+-doped ZnO-B2O3-P2O5 glasses

    Science.gov (United States)

    Jayasimhadri, M.; Jang, Kiwan; Lee, Ho Sueb; Chen, Baojiu; Yi, Soung-Soo; Jeong, Jung-Hyun

    2009-07-01

    Dysprosium doped ZnO-B2O3-P2O5 (ZBP) glasses were prepared by a conventional melt quenching technique in order to study the luminescent properties and their utility for white light emitting diodes (LEDs). X-ray diffraction spectra revealed the amorphous nature of the glass sample. The present glasses were characterized by infrared and Raman spectra to evaluate the vibrational features of the samples. The emission and excitation spectra were reported for the ZBP glasses. Strong blue (484 nm) and yellow (574 nm) emission bands were observed upon various excitations. These two emissions correspond to the F49/2→H615/2 and F49/2→H613/2 transitions of Dy3+ ions, respectively. Combination of these blue and yellow bands gives white light to the naked eye. First time, it was found that ZnO-B2O3-P2O5 glasses efficiently emit white light under 400 and 454 nm excitations, which are nearly match with the emissions of commercial GaN blue LEDs and InGaN LED, respectively. CIE chromaticity coordinates also calculated for Dy3+: ZBP glasses to evaluate the white light emission.

  2. Bonding and site preferences in the new quasi-binary Zr{sub 2.7}Hf{sub 11.3}P{sub 9}

    Energy Technology Data Exchange (ETDEWEB)

    Kleinke, H.; Franzen, H.F. [Ames Lab., IA (United States)

    1998-03-01

    The new quasi-binary Zr{sub 2.7}Hf{sub 11.3}P{sub 9} was synthesized by arc-melting of Zr, Hf, Co, and HfP in a ratio corresponding to the initial composition Zr{sub 2.25}Hf{sub 6.75}Co{sub 2}P{sub 4}. Zr{sub 2.7}Hf{sub 11.3}P{sub 9} crystallizes in the Zr{sub 14}P{sub 9} structure type, which is unknown in the binary Hf/P system. the ideal orthorhomibic lattice dimensions (space group Pnnm (No. 58), Z = 4) were refined to a = 16.640(7) {angstrom}, b = 27.40(2) {angstrom}, c = 3.619(1) {angstrom}, V = 1650(2) {angstrom}{sup 3}. The structure consists of three-dimensional condensed one-, two-, and three-capped trigonal (Zr, Hf){sub 6}P prisms, occurring with numerous short M-M bonds (M = Zr, Hf). Each of the 15 metal sites is statistically occupied by a mixture of Zr and Hf, which varies significantly from site to site. The Hf/Zr ratio in a given site depends on the M-M and M-P interactions. The systematic increase of this ratio with increasing total bond order, as evaluated via Mulliken overlap populations and Pauling bond orders, can be understood based on the trend that Hf forms stronger M-M and M-P bonds than Zr. As expected for a metal-rich phosphide, band structure calculations for the hypothetical Hf{sub 14}P{sub 9} carried out with the extended Hueckel approximation result in a significant density of states at the Fermi level.

  3. Estimative of core damage frequency in IPEN IEA-R1 research reactor due to the initiating events of loss of flow caused by channel blockage and loss of coolant caused by a large rupture in the pipe of the primary circuit - PSA level 1

    Energy Technology Data Exchange (ETDEWEB)

    Hirata, Daniel Massami [Centro Tecnologico da Marinha em Sao Paulo (CTMSP), Sao Paulo, SP (Brazil); Sabundjian, Gaiane, E-mail: gdjian@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP) Sao Paulo, SP (Brazil)

    2011-07-01

    This work applies the methodology of Probabilistic Safety Assessment Level 1 to the research reactor IEA-R1 IPEN-CNEN/SP. Two categories of identified initiating events of accidents in the reactor are studied: loss of flow and loss of primary coolant. Among the initiating events, blockage of flow channel and loss of cooling fluid caused by large pipe rupture in the primary circuit are chosen for a detailed analysis. The event tree technique is used to analyze the evolution of the accident, including the actuation or the fail of actuation of the safety systems and the reactor damages. Using the fault tree the reliability of the following reactor safety systems is evaluated: reactor shutdown system, isolation of the reactor pool, Emergency Core Cooling System (ECCS) and the electric system. Estimative for the frequency of damage to the reactor core and the probability of failure of the analyzed systems are calculated. The estimated values for the frequencies of core damage are within the expected margins and are of the same order of magnitude as those found for similar reactors. The reliability of the reactor shutdown system, isolation of the reactor pool and ECCS are satisfactory for the conditions in which these systems are required. However, for the electric system it is suggested an upgrade to increase its reliability. (author)

  4. Application of Core Dynamics Modeling to Core-Mantle Interactions

    Science.gov (United States)

    Kuang, Weijia

    2003-01-01

    Observations have demonstrated that length of day (LOD) variation on decadal time scales results from exchange of axial angular momentum between the solid mantle and the core. There are in general four core-mantle interaction mechanisms that couple the core and the mantle. Of which, three have been suggested likely the dominant coupling mechanism for the decadal core-mantle angular momentum exchange, namely, gravitational core-mantle coupling arising from density anomalies in the mantle and in the core (including the inner core), the electromagnetic coupling arising from Lorentz force in the electrically conducting lower mantle (e.g. D-layer), and the topographic coupling arising from non-hydrostatic pressure acting on the core-mantle boundary (CMB) topography. In the past decades, most effort has been on estimating the coupling torques from surface geomagnetic observations (kinematic approach), which has provided insights on the core dynamical processes. In the meantime, it also creates questions and concerns on approximations in the studies that may invalidate the corresponding conclusions. The most serious problem is perhaps the approximations that are inconsistent with dynamical processes in the core, such as inconsistencies between the core surface flow beneath the CMB and the CMB topography, and that between the D-layer electric conductivity and the approximations on toroidal field at the CMB. These inconsistencies can only be addressed with numerical core dynamics modeling. In the past few years, we applied our MoSST (Modular, Scalable, Self-consistent and Three-dimensional) core dynamics model to study core-mantle interactions together with geodynamo simulation, aiming at assessing the effect of the dynamical inconsistencies in the kinematic studies on core-mantle coupling torques. We focus on topographic and electromagnetic core-mantle couplings and find that, for the topographic coupling, the consistency between the core flow and the CMB topography is

  5. Extensive and accurate energy levels and transition rates for Al-like Zn XVIII

    Science.gov (United States)

    Si, R.; Zhang, C. Y.; Liu, Y. W.; Chen, Z. B.; Guo, X. L.; Li, S.; Yan, J.; Chen, C. Y.; Wang, K.

    2017-03-01

    Energy levels and transition rates for electric-dipole (E1), electric-quadrupole (E2), magnetic-dipole (M1), and magnetic-quadrupole (M2) transitions of the lowest 393 levels arising from the 3l3 (0 ≤ l ⩽ 2), 3s2 4 l (0 ≤ l ⩽ 3), 3 s 3 p 4 l (0 ≤ l ⩽ 3), 3p2 4 l (0 ≤ l ⩽ 2), 3 s 3 d 4 l (0 ≤ l ⩽ 1), and 3s2 5 l (0 ≤ l ⩽ 4) configurations in Al-like Zn are calculated through the multi-configuration Dirac-Hartree-Fock (MCDHF) method and second-order many-body perturbation theory (MBPT). In the MCDHF calculation, valence-valence and core-valence correlations with the 2 p and 2 s electrons are taken into account. The effect of Breit interaction and quantum electrodynamics corrections on excitation level energies and level lifetimes are assessed though the MCDHF and MBPT calculations. The two sets of level energies are in excellent agreement of better than 0.1%, while the level lifetimes mostly agree to within 2%. Comparisons are also made with experimental measurements and other theoretical results to assess the accuracy of our calculations.

  6. USGS Core Research Center (CRC) Collection of Core

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The Core Research Center (CRC) was established in 1974 by the U.S. Geological Survey (USGS) to preserve valuable rock cores for use by scientists and educators from...

  7. Ice Cores of the National Ice Core Laboratory

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The U.S. National Ice Core Laboratory (NICL) is a facility for storing, curating, and studying ice cores recovered from the polar regions of the world. It provides...

  8. Core-shell microparticles for protein sequestration and controlled release of a protein-laden core.

    Science.gov (United States)

    Rinker, Torri E; Philbrick, Brandon D; Temenoff, Johnna S

    2016-12-21

    Development of multifunctional biomaterials that sequester, isolate, and redeliver cell-secreted proteins at a specific timepoint may be required to achieve the level of temporal control needed to more fully regulate tissue regeneration and repair. In response, we fabricated core-shell heparin-poly(ethylene-glycol) (PEG) microparticles (MPs) with a degradable PEG-based shell that can temporally control delivery of protein-laden heparin MPs. Core-shell MPs were fabricated via a re-emulsification technique and the number of heparin MPs per PEG-based shell could be tuned by varying the mass of heparin MPs in the precursor PEG phase. When heparin MPs were loaded with bone morphogenetic protein-2 (BMP-2) and then encapsulated into core-shell MPs, degradable core-shell MPs initiated similar C2C12 cell alkaline phosphatase (ALP) activity as the soluble control, while non-degradable core-shell MPs initiated a significantly lower response (85+19% vs. 9.0+4.8% of the soluble control, respectively). Similarly, when degradable core-shell MPs were formed and then loaded with BMP-2, they induced a ∼7-fold higher C2C12 ALP activity than the soluble control. As C2C12 ALP activity was enhanced by BMP-2, these studies indicated that degradable core-shell MPs were able to deliver a bioactive, BMP-2-laden heparin MP core. Overall, these dynamic core-shell MPs have the potential to sequester, isolate, and then redeliver proteins attached to a heparin core to initiate a cell response, which could be of great benefit to tissue regeneration applications requiring tight temporal control over protein presentation.

  9. Some core contested concepts.

    Science.gov (United States)

    Chomsky, Noam

    2015-02-01

    Core concepts of language are highly contested. In some cases this is legitimate: real empirical and conceptual issues arise. In other cases, it seems that controversies are based on misunderstanding. A number of crucial cases are reviewed, and an approach to language is outlined that appears to have strong conceptual and empirical motivation, and to lead to conclusions about a number of significant issues that differ from some conventional beliefs.

  10. Reaction Dynamics of O((3)P) + Propyne: I. Primary Products, Branching Ratios, and Role of Intersystem Crossing from Crossed Molecular Beam Experiments.

    Science.gov (United States)

    Vanuzzo, Gianmarco; Balucani, Nadia; Leonori, Francesca; Stranges, Domenico; Nevrly, Vaclav; Falcinelli, Stefano; Bergeat, Astrid; Casavecchia, Piergiorgio; Cavallotti, Carlo

    2016-07-14

    We performed synergic experimental/theoretical studies on the mechanism of the O((3)P) + propyne reaction by combining crossed molecular beams experiments with mass-spectrometric detection and time-of-flight analysis at 9.2 kcal/mol collision energy (Ec) with ab initio electronic structure calculations at a high level of theory of the relevant triplet and singlet potential energy surfaces (PESs) and statistical calculations of branching ratios (BRs) taking into account intersystem crossing (ISC). In this paper (I) we report the results of the experimental investigation, while the accompanying paper (II) shows results of the theoretical investigation with comparison to experimental results. By exploiting soft electron ionization detection to suppress/mitigate the effects of the dissociative ionization of reactants, products, and background gases, product angular and velocity distributions at different charge-to-mass ratios were measured. From the laboratory data angular and translational energy distributions in the center-of-mass system were obtained for the five competing most important product channels, and product BRs were derived. The reactive interaction of O((3)P) with propyne under single collision conditions is mainly leading to the rupture of the three-carbon atom chain, with production of the radical products methylketenyl + atomic hydrogen (BR = 0.04), methyl + ketenyl (BR = 0.10), and vinyl + formyl (BR = 0.11) and the molecular products ethylidene/ethylene + carbon monoxide (BR = 0.74) and propandienal + molecular hydrogen (BR = 0.01). Because some of the products can only be formed via ISC from the entrance triplet to the low-lying singlet PES, we infer from their BRs an amount of ISC larger than 80%. This value is dramatically large when compared to the negligible ISC reported for the O((3)P) reaction with the simplest alkyne, acetylene. At the same time, it is much larger than that (∼20%) recently observed in the related reaction of the three

  11. Central core disease

    Directory of Open Access Journals (Sweden)

    Jungbluth Heinz

    2007-05-01

    Full Text Available Abstract Central core disease (CCD is an inherited neuromuscular disorder characterised by central cores on muscle biopsy and clinical features of a congenital myopathy. Prevalence is unknown but the condition is probably more common than other congenital myopathies. CCD typically presents in infancy with hypotonia and motor developmental delay and is characterized by predominantly proximal weakness pronounced in the hip girdle; orthopaedic complications are common and malignant hyperthermia susceptibility (MHS is a frequent complication. CCD and MHS are allelic conditions both due to (predominantly dominant mutations in the skeletal muscle ryanodine receptor (RYR1 gene, encoding the principal skeletal muscle sarcoplasmic reticulum calcium release channel (RyR1. Altered excitability and/or changes in calcium homeostasis within muscle cells due to mutation-induced conformational changes of the RyR protein are considered the main pathogenetic mechanism(s. The diagnosis of CCD is based on the presence of suggestive clinical features and central cores on muscle biopsy; muscle MRI may show a characteristic pattern of selective muscle involvement and aid the diagnosis in cases with equivocal histopathological findings. Mutational analysis of the RYR1 gene may provide genetic confirmation of the diagnosis. Management is mainly supportive and has to anticipate susceptibility to potentially life-threatening reactions to general anaesthesia. Further evaluation of the underlying molecular mechanisms may provide the basis for future rational pharmacological treatment. In the majority of patients, weakness is static or only slowly progressive, with a favourable long-term outcome.

  12. Drilling history core hole DC-6 Hanford, Washington

    Energy Technology Data Exchange (ETDEWEB)

    1978-06-01

    Core hole DC-6 was completed in May 1978 by Boyles Brothers Drilling Company, Spokane, Washington, under subcontract to Fenix and Scisson, Inc. The hole was cored for the US Department of Energy and the Rockwell Hanford Operations' Basalt Waste Isolation Program. Fenix and Scisson, Inc. furnished the engineering, daily supervision of the core drilling activities, and geologic core logging for hole DC-6. Core hole DC-6 is located within the boundary of the Hanford Site at the old Hanford town site. The Hanford Site coordinates for DC-6 are North 54,127.17 feet and West 17,721.00 feet. The surface elevation is approximately 402 feet above sea level. The purpose of core hole DC-6 was to core drill vertically through the basalt and interbed units for stratigraphic depth determination and core collection and to provide a borehole for hydrologic testing. The total depth of core hole DC-6 was 4336 feet. Core recovery was 98.4% of the total footage cored.

  13. GHRSST Level 3P North Atlantic Regional Subskin Sea Surface Temperature from the Advanced Very High Resolution Radiometer (AVHRR) on NOAA-19 (GDS version 1)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A Group for HIgh Resolution Sea Surface Temperature (GHRSST) dataset for the North Atlantic Region (NAR) from the Advanced Very High Resolution Radiometer (AVHRR) on...

  14. THE FUNGAL HYDROPHOBIN SC3P SELF-ASSEMBLES AT THE SURFACE OF AERIAL HYPHAE AS A PROTEIN MEMBRANE CONSTITUTING THE HYDROPHOBIC RODLET LAYER

    NARCIS (Netherlands)

    WOSTEN, HAB; ASGEIRSDOTTIR, SA; KROOK, JH; DRENTH, JHH; WESSELS, JGH

    1994-01-01

    The Schizophyllum commune hydrophobin Sc3p is a small, hydrophobic, cysteine-rich protein involved in the formation of aerial hyphae. Using an antibody against purified Sc3p we found that the hydrophobin is secreted into the medium at the spices of growing submerged hyphae but in emerging aerial hyp

  15. Reactions of R(2)P-P(SiMe(3))Li with [(R'(3)P)(2)PtCl(2)]. A general and efficient entry to phosphanylphosphinidene complexes of platinum. Syntheses and structures of [(eta(2)-P=(i)Pr(2))Pt(p-Tol(3)P)(2)], [(eta(2)-P=(t)Bu(2))Pt(p-Tol(3)P)(2)], [{eta(2)-P=(N(i)Pr(2))(2)}Pt(p-Tol(3)P)(2)] and [{(Et(2)PhP)(2)Pt}(2)P(2)].

    Science.gov (United States)

    Domańska-Babul, Wioleta; Chojnacki, Jaroslaw; Matern, Eberhard; Pikies, Jerzy

    2009-01-07

    The reactions of lithium derivatives of diphosphanes R(2)P-P(SiMe(3))Li (R = (t)Bu, (i)Pr, Et(2)N and (i)Pr(2)N) with [(R'(3)P)(2)PtCl(2)] (R'(3)P = Et(3)P, Et(2)PhP, EtPh(2)P and p-Tol(3)P) proceed in a facile manner to afford side-on bonded phosphanylphosphinidene complexes of platinum [(eta(2)-P=R(2))Pt(PR'(3))(2)]. The related reactions of Ph(2)P-P(SiMe(3))Li with [(R'(3)P)(2)PtCl(2)] did not yield [(eta(2)-P=PPh(2))Pt(PR'(3))(2)] and resulted mainly in the formation of [{(R'(3)P)(2)Pt}(2)P(2)], Ph(2)P-PLi-PPh(2), (Me(3)Si)(2)PLi and (Me(3)Si)(3)P. Crystallographic data are reported for the compounds [(eta(2)-P=R(2))Pt(p-Tol(3)P)(2)] (R = (t)Bu, (i)Pr, ((i)Pr(2)N)(2)P) and for [{(Et(2)PhP)(2)Pt}(2)P(2)].

  16. Neanderthal introgression at chromosome 3p21.31 was under positive natural selection in East Asians.

    Science.gov (United States)

    Ding, Qiliang; Hu, Ya; Xu, Shuhua; Wang, Jiucun; Jin, Li

    2014-03-01

    Studies of the Neanderthal and Denisovan genomes demonstrate archaic hominin introgression in Eurasians. Here, we present evidence of Neanderthal introgression within the chromosome 3p21.31 region, occurring with a high frequency in East Asians (ranging from 49.4% to 66.5%) and at a low frequency in Europeans. We also detected a signal of strong positive selection in this region only in East Asians. Our data indicate that likely candidate targets of selection include rs12488302-T and its associated alleles--among which four are nonsynonymous, including rs35455589-G in HYAL2, a gene related to the cellular response to ultraviolet-B irradiation. Furthermore, suggestive evidence supports latitude-dependent selection, implicating a role of ultraviolet-B. Interestingly, the distribution of rs35455589-G suggests that this allele was lost during the exodus of ancestors of modern Eurasians from Africa and reintroduced to Eurasians from Neanderthals.

  17. Kinetics and thermodynamic behavior of WO{sub 3} and WO{sub 3}:P thin films

    Energy Technology Data Exchange (ETDEWEB)

    Avellaneda, Cesar O.; Bulhoes, Luis O.S. [Laboratorio Interdisciplinar de Eletroquimica e Ceramica-LIEC, Departamento de Quimica, Universidade Federal de Sao Carlos, C.P. 676, CEP 13565-905, Sao Carlos-SP (Brazil)

    2006-03-06

    There is a considerable interest in the research and development of materials and devices, that can be used for optical switching of large-scale glazings. Several potential switching technologies are available for glazings, including those based on electrochromic, thermochromic and photochromic phenomena. One of the most promising technologies for optical switching devices is electrochromism (EC). In order to improve the electrochromic properties of tungsten oxide, we have investigated the effect of phosphorous insertion on the electrochromic behavior of oxide films prepared by the sol-gel process. The kinetics and thermodynamics of electrochemical intercalation of lithium into Li{sub x}WO{sub 3} and Li{sub x}WO{sub 3}:P films prepared by the sol-gel process were investigated. The standard Gibbs energy for lithium intercalation was calculated. The chemical diffusion coefficients, D, of lithium intercalation into oxide, were measured by galvanostatic intermittent titration technique (GITT), as functions of the depth of lithium intercalation. (author)

  18. Magnetic control of ultra-cold $^6$Li and $^{174}$Yb($^3P_2$) atom mixtures with Feshbach resonances

    CERN Document Server

    Petrov, Alexander; Kotochigova, Svetlana

    2015-01-01

    We theoretically evaluate the feasibility to form magnetically-tunable Feshbach molecules in collisions between fermionic $^6$Li atoms and bosonic metastable $^{174}$Yb($^3$P$_2$) atoms. In contrast to the well-studied alkali-metal atom collisions, collisions with meta-stable atoms are highly anisotropic. Our first-principle coupled-channel calculation of these collisions reveals the existence of broad Feshbach resonances due to the combined effect of anisotropic-molecular and atomic-hyperfine interactions. In order to fit our predictions to the specific positions of experimentally-observed broad resonance structures \\cite{Deep2015} we optimized the shape of the short-range potentials by direct least-square fitting. This allowed us to identify the dominant resonance by its leading angular momentum quantum numbers and describe the role of collisional anisotropy in the creation and broadening of this and other resonances.

  19. Photoconductivity of novel poly(N-vinyl)-3-[p-nitrophenylazo]carbazole/CdS-nanoparticle polymer composite

    Institute of Scientific and Technical Information of China (English)

    DING Li-yun; JIANG De-sheng; HUANG Jun; DAI Lei; LIU Cheng; WANG Jun-tao; LI Bin

    2006-01-01

    The photoconductive characteristic of the inorganic/organic hybridized polymer system is reported,in which a novel bi-functional photorefractive (PR) poly(N-vinyl)-3-[p-nitrophenylazo]carbazole (PVNPAK) serves as a polymeric charge-transporting and second-order nonliner optical matrix and quantum dots composed of surface passivated cadmium sulfide serve as a charge-generation sensitizer. The hybrid PVNPAK/CdS-nanoparticles polymer composites with different mass ratio of CdS to PVNPAK were prepared. The generation of photocurrent on illumination and photoconductive properties of the PVNPAK/CdS-nanoparticles polymer composites were studied. The results show that the addition of CdS nanoparticle as a photosensitizer can enhance the photoconductivity of the PVNPAK significantly because of the properties of the high quantum efficiency of photosensitization and high charge transport to conducting polymer.

  20. Localization of the human {beta}-catenin gene (CTNNB1) to 3p21: A region implicated in tumor development

    Energy Technology Data Exchange (ETDEWEB)

    Kraus, C.; Liehr, T.; Ballhausen, G. [Institut fuer Humangenetik der Universitaet, Erlangen (Germany)] [and others

    1994-09-01

    The human {beta}-catenin locus (CTNNB1) was mapped by in situ fluorescence analysis to band p21 on the short arm of chromosome 3, a region frequently affected by somatic alterations in a variety of tumors. PCR primers for the genomic amplification of {beta}-catenin sequences were selected on the basis of homology to exon 4 of the Drosophila armadillo gene. Analysis of a panel of somatic cell hybrids confirmed the localization of {beta}-catenin on human chromosome 3. Furthermore, exclusion mapping of three hybrids carrying defined fragments of the short arm of human chromosome 3 allowed us to determine the position of the CTNNB1 locus close to the marker D3S2 in 3p21. 22 refs., 3 figs.

  1. Atomic scattering in the diffraction limit: electron transfer in keV Li+-Na(3s, 3p) collisions

    DEFF Research Database (Denmark)

    Poel, Mike van der; Nielsen, C.V.; Rybaltover, M.;

    2002-01-01

    . This setup yields a momentum resolution of 0.12 an, an order of magnitude better angular resolution than previous measurements on this system. This enables us to clearly resolve Fraunhofer-type diffraction patterns in the angle DCS. In particular, the angular width of the ring structure is given by the ratio...... of the de Broglie wavelength lambda(dB) = 150 fm at a velocity v = 0.20 au and the effective atomic diameter for electron capture 2R = 20 au. Parallel AO and MO semiclassical coupled-channel calculations of the Na(3s, 3p) --> Li(2s, 2p) state-to-state collision amplitudes have been performed, and quantum...

  2. The C(3P) + NH3 reaction in interstellar chemistry: I. Investigation of the product formation channels

    CERN Document Server

    Bourgalais, Jeremy; Kailasanathan, Ranjith Kumar Abhinavam; Osborn, David L; Hickson, Kevin M; Loison, Jean-Christophe; Wakelam, Valentine; Goulay, Fabien; Picard, Sébastien D Le

    2016-01-01

    The product formation channels of ground state carbon atoms, C(3P), reacting with ammonia, NH3, have been investigated using two complementary experiments and electronic structure calculations. Reaction products are detected in a gas flow tube experiment (330 K, 4 Torr) using tunable VUV photoionization coupled with time of flight mass spectrometry. Temporal profiles of the species formed and photoionization spectra are used to identify primary products of the C + NH3 reaction. In addition, H-atom formation is monitored by VUV laser induced fluorescence from room temperature to 50 K in a supersonic gas flow generated by the Laval nozzle technique. Electronic structure calculations are performed to derive intermediates, transition states and complexes formed along the reaction coordinate. The combination of photoionization and laser induced fluorescence experiments supported by theoretical calculations indicate that in the temperature and pressure range investigated, the H + H2CN production channel represents ...

  3. Core-seis: a code for LMFBR core seismic analysis

    Energy Technology Data Exchange (ETDEWEB)

    Chellapandi, P.; Ravi, R.; Chetal, S.C.; Bhoje, S.B. [Indira Gandhi Centre for Atomic Research, Kalpakkam (India). Reactor Group

    1995-12-31

    This paper deals with a computer code CORE-SEIS specially developed for seismic analysis of LMFBR core configurations. For demonstrating the prediction capability of the code, results are presented for one of the MONJU reactor core mock ups which deals with a cluster of 37 subassemblies kept in water. (author). 3 refs., 7 figs., 2 tabs.

  4. Core Exercises: Why You Should Strengthen Your Core Muscles

    Science.gov (United States)

    Healthy Lifestyle Fitness You know core exercises are good for you — but do you include core exercises in your fitness routine? Here's why ... 18, 2014 Original article: http://www.mayoclinic.org/healthy-lifestyle/fitness/in-depth/core-exercises/art-20044751 . Mayo Clinic Footer Legal Conditions and ...

  5. MiRNA-365 and miRNA-520c-3p respond to risperidone treatment in first-episode schizophrenia after a 1 year remission

    Institute of Scientific and Technical Information of China (English)

    LIU Sha; YUAN Yan-bo; GUAN Li-li; WEI Hui; CHENG Zhang; HAN Xue; YANG Lei

    2013-01-01

    Background MicroRNAs (miRNAs) control gene expression by destabilizing target transcripts and inhibiting their translation.Aberrant expression of miRNAs has been described in many human diseases,including schizophrenia.However,the effects on miRNA expression in response to antipsychotic treatment in peripheral circulation have not been thoroughly examined.Methods Using quantitative real-time PCR (qRT-PCR),We quantified the expression of seven candidate miRNAs in plasma samples of 40 first-episode schizophrenics before and after antipsychotic treatment.The patients were all treated with risperidone and achieved remission in 1 year.Results Compared with the baseline,the expression levels of miR-365 and miR-520c-3p were significantly downregulated after 1 year of risperidone treatment (P <0.001).There were no significant correlations between the clinical symptoms and the expression levels of these two miRNAs (P >0.05).Conclusions This study analyzed possible circulating miRNAs in response to antipsychotic monotherapy for schizophrenia,the further mechanism need to be confirmed.

  6. TamA interacts with LeuB, the homologue of Saccharomyces cerevisiae Leu3p, to regulate gdhA expression in Aspergillus nidulans.

    Science.gov (United States)

    Polotnianka, R; Monahan, B J; Hynes, M J; Davis, M A

    2004-11-01

    Previous studies have shown that expression of the gdhA gene, encoding NADP-linked glutamate dehydrogenase (NADP-GDH), in Aspergillus nidulans is regulated by the major nitrogen regulatory protein AreA and its co-activator TamA. We show here that loss of TamA function has a more severe effect on the levels of gdhA expression than loss of AreA function. Using TamA as the bait in a yeast two-hybrid screen, we have identified a second protein that interacts with TamA. Sequencing analysis and functional studies have shown that this protein, designated LeuB, is a transcriptional activator with similar function to the homologous Leu3p of Saccharomyces cerevisiae. Inactivation of leuB revealed that this gene is involved in the regulation of gdhA, and an areA; leuB double mutant was shown to have similar NADP-GDH levels to a tamA single mutant. The requirement for TamA function to promote gdhA expression is likely to be due to its dual interaction with AreA and LeuB.

  7. Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates

    Directory of Open Access Journals (Sweden)

    Emmanuel M Papamichael

    1999-01-01

    Full Text Available In this study, the S1 - P1 and S3 - P3 interactions between papain and four synthetic peptide substrates were found as to be important. The values of Km were estimated as to be practically identical between these substrates; this latter is supporting the conclusions obtained by considering the estimated values of other kinetic parameters. Nevertheless, based on the estimated kcat and/or k cat/Km parameters of the used substrates, we concluded that an aromatic ring at the P3 position, and a positively charged side chain of the residue at the P1 position of the synthetic substrates were favored considerably their interaction with papain.Neste estudo, o S1 - P1 e S3 - P3, interações entre papaina e quatro substratos sintéticos de pepetídios foram considerados importantes. Os valores de Km foram estimados e são praticamente idênticos entre esses substratos; Isso dá suporte as conclusões obtidas, considerando os valores parâmetros cinéticos estimados. No obstante, baseou na estimação parâmetros kcat e/ou k cat /Km dos substratos utilizados. Se pode concluir que um anel aromático na posição P3, e uma corrente carregada positivamente da cadeia do resíduo na posição P1 dos substratos sintéticos favoreceram interação com a papaina.

  8. Major weapon system environmental life-cycle cost estimating for Conservation, Cleanup, Compliance and Pollution Prevention (C3P2)

    Science.gov (United States)

    Hammond, Wesley; Thurston, Marland; Hood, Christopher

    1995-01-01

    The Titan 4 Space Launch Vehicle Program is one of many major weapon system programs that have modified acquisition plans and operational procedures to meet new, stringent environmental rules and regulations. The Environmental Protection Agency (EPA) and the Department of Defense (DOD) mandate to reduce the use of ozone depleting chemicals (ODC's) is just one of the regulatory changes that has affected the program. In the last few years, public environmental awareness, coupled with stricter environmental regulations, has created the need for DOD to produce environmental life-cycle cost estimates (ELCCE) for every major weapon system acquisition program. The environmental impact of the weapon system must be assessed and budgeted, considering all costs, from cradle to grave. The Office of the Secretary of Defense (OSD) has proposed that organizations consider Conservation, Cleanup, Compliance and Pollution Prevention (C(sup 3)P(sup 2)) issues associated with each acquisition program to assess life-cycle impacts and costs. The Air Force selected the Titan 4 system as the pilot program for estimating life-cycle environmental costs. The estimating task required participants to develop an ELCCE methodology, collect data to test the methodology and produce a credible cost estimate within the DOD C(sup 3)P(sup 2) definition. The estimating methodology included using the Program Office weapon system description and work breakdown structure together with operational site and manufacturing plant visits to identify environmental cost drivers. The results of the Titan IV ELCCE process are discussed and expanded to demonstrate how they can be applied to satisfy any life-cycle environmental cost estimating requirement.

  9. miR-373-3p Targets DKK1 to Promote EMT-Induced Metastasis via the Wnt/β-Catenin Pathway in Tongue Squamous Cell Carcinoma

    Science.gov (United States)

    Zhang, Hui; Wang, Cheng; Liang, Jianfeng; Chen, Guanhui; Li, Wenqing; Tang, Haikuo

    2017-01-01

    MicroRNAs (miRNAs) regulate gene expression and at the same time mediate tumorigenesis. miR-373-3p has diverse effects in tumors, but its role in tongue squamous cell carcinoma (TSCC) remains unknown. The purpose of this study is to determine the function of miR-373-3p in the progression of TSCC. Our results brought to light that miR-373-3p is markedly upregulated in clinical TSCC tissues compared with paired adjacent normal tissues and has significant correlation with a more aggressive TSCC phenotype in patients. Gain-of-function and loss-of-function studies revealed that ectopic miR-373-3p overexpression promoted the metastasis of TSCC cells. Notably, Wnt/β-catenin signaling was hyperactivated in TSCC cells overexpressing miR-373-3p, and this pathway was responsible for the epithelial-mesenchymal transition (EMT) induced by miR-373-3p. Furthermore, miR-373-3p directly targeted and suppressed Dickkopf-1 (DKK1), a negative regulator of the Wnt/β-catenin signaling cascade. These results demonstrate that, by directly targeting DKK1, miR-373-3p constitutively activated Wnt/β-catenin signaling, thus promoting the EMT-induced metastasis of TSCC. Taken together, our findings reveal a new regulatory mechanism for miR-373-3p and suggest that miR-373-3p might be a potential target in TSCC therapy.

  10. Mapping a gene that determines erythrocytic GTP concentration to a region of mouse chromosome 9 which is syntenic to human chromosome 3p

    Energy Technology Data Exchange (ETDEWEB)

    Snyder, F.F.; Jenuth, J.P.; Noy, J.L. [Univ. of Calgary, Alberta (Canada)] [and others

    1994-09-01

    Inbred mouse strains were surveyed for erythrocytic GTP concentration by high performance liquid chromatography and found to fall into two groups. Strains having low GTP levels between 1.4-3.4 nmole/10{sup 9} cells are represented by C3H/HeJ. Strains having high GTP levels between 11.0 - 14.8 nmole/10{sup 9} cells are represented by C57BL/6J. Erythocytic ATP levels did not vary significantly among these strains (63-87 nmole/10{sup 9} cells). Crosses between low and high GTP strains gave F{sub 1} progeny having intermediate levels of GTP. The progeny of F{sub 1}`s backcrossed to paternal strains segregated in a 1:1 ratio for GTP concentration characteristic of the F{sub 1} and parental strain. We designated the GTP concentration-determining trait Gtpc. Typing of the twelve BXH recombinant inbred strains revealed 0/12 strain distribution pattern differences with Gtpc for loci on both chromosomes 5 and 9. Backcross analysis did not provide evidence for linkage of Gtpc to W (dominant white spotting) on chromosome 5 with 15/45 recombinants. Backcross analysis testing for linkage of Gtpc to transferrin (Trf) on chromosome 9 gave evidence for linkage with a recombination frequency of 9.68 {plus_minus} 3.07. DNA-based typing of repeat length polymorphic markers on chromosome 9 gave a map distance of 10.7 {plus_minus} 3.6 between D9 MITl4 and Gtpc and placed Gtpc on the telomeric side of Trf. This region of mouse chromosome 9 is syntenic to human chromosome 3p and encompasses a cluster of G-protein loci.

  11. Physical property data from the ICDP-USGS Eyreville cores A and B, Chesapeake Bay impact structure, Virginia, USA, acquired using a multisensor core logger

    Science.gov (United States)

    Pierce, H.A.; Murray, J.B.

    2009-01-01

    The International Continental Scientific Drilling Program (ICDP) and the U.S. Geological Survey (USGS) drilled three core holes to a composite depth of 1766 m within the moat of the Chesapeake Bay impact structure. Core recovery rates from the drilling were high (??90%), but problems with core hole collapse limited the geophysical downhole logging to natural-gamma and temperature logs. To supplement the downhole logs, ??5% of the Chesapeake Bay impact structure cores was processed through the USGS GeoTek multisensor core logger (MSCL) located in Menlo Park, California. The measured physical properties included core thickness (cm), density (g cm-3), P-wave velocity (m s-1), P-wave amplitude (%), magnetic susceptibility (cgs), and resistivity (ohm-m). Fractional porosity was a secondary calculated property. The MSCL data-sampling interval for all core sections was 1 cm longitudinally. Photos of each MSCL sampled core section were imbedded with the physical property data for direct comparison. These data have been used in seismic, geologic, thermal history, magnetic, and gravity models of the Chesapeake Bay impact structure. Each physical property curve has a unique signature when viewed over the full depth of the Chesapeake Bay impact structure core holes. Variations in the measured properties reflect differences in pre-impact target-rock lithologies and spatial variations in impact-related deformation during late-stage crater collapse and ocean resurge. ?? 2009 The Geological Society of America.

  12. miR-199a-3p targets CD44 and reduces proliferation of CD44 positive hepatocellular carcinoma cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Henry, Jon C. [Department of Surgery, Ohio State University Medical Center, Columbus, OH (United States); Park, Jong-Kook; Jiang, Jinmai; Kim, Ji Hye [College of Pharmacy, Ohio State University, Columbus, OH (United States); Nagorney, David M.; Roberts, Lewis R. [Divisions of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, Rochester, MN (United States); Banerjee, Soma [Center for Liver Research, School of Digestive and Liver Diseases, Institute of Post Graduate Medical Education and Research, Kolkata (India); Schmittgen, Thomas D., E-mail: Schmittgen.2@osu.edu [College of Pharmacy, Ohio State University, Columbus, OH (United States)

    2010-12-03

    Research highlights: {yields} miR-199a-3p targets CD44 in HCC. {yields} Proliferation and invasion are reduced by miR-199a-3p in CD44+ HCC. {yields} miR-199a-3p is reduced and CD44 protein is increased in HCC tissues. {yields} The duplex form of miR-199a-3p mimetic is required for activity. -- Abstract: Previous work by us and others reported decreased expression of miR-199a-3p in hepatocellular carcinoma (HCC) tissues compared to adjacent benign tissue. We report here a significant reduction of miR-199a-3p expression in 7 HCC cell lines. To determine if miR-199a-3p has a tumor suppressive role, pre-miR-199a-3p oligonucleotides were transfected into the HCC cell lines. Pre-miR-199a-3p oligonucleotide reduced cell proliferation by approximately 60% compared to control oligonucleotide in only two cell lines (SNU449 and SNU423); the proliferation of the other 5 treated cell lines was similar to control oligonucleotide. A pre-miR-199a-3p oligonucleotide formulated with chemical modifications to enhance stability while preserving processing, reduced cell proliferation in SNU449 and SNU423 to the same extent as the commercially available pre-miR-199a-3p oligonucleotide. Furthermore, only the duplex miR-199a-3p oligonucleotide, and not the guide strand alone, was effective at reducing cell viability. Since a CD44 variant was essential for c-Met signaling [V. Orian-Rousseau, L. Chen, J.P. Sleeman, P. Herrlich, H. Ponta, CD44 is required for two consecutive steps in HGF/c-Met signaling, Genes Dev. 16 (2002) 3074-3086] and c-Met is a known miR-199a-3p target, we hypothesized that miR-199a-3p may also target CD44. Immunoblotting confirmed that only the two HCC lines that were sensitive to the effects of pre-miR-199a-3p were CD44+. Direct targeting of CD44 by miR-199a-3p was confirmed using luciferase reporter assays and immunoblotting. Transfection of miR-199a-3p into SNU449 cells reduced in vitro invasion and sensitized the cells to doxorubicin; both effects were enhanced

  13. On core stability and extendability

    OpenAIRE

    Shellshear, Evan

    2007-01-01

    This paper investigates conditions under which the core of a TU cooperative game is stable. In particular the author extends the idea of extendability to find new conditions under which the core is stable. It is also shown that these new conditions are not necessary for core stability.

  14. Hollow-Core Fiber Lamp

    Science.gov (United States)

    Yi, Lin (Inventor); Tjoelker, Robert L. (Inventor); Burt, Eric A. (Inventor); Huang, Shouhua (Inventor)

    2016-01-01

    Hollow-core capillary discharge lamps on the millimeter or sub-millimeter scale are provided. The hollow-core capillary discharge lamps achieve an increased light intensity ratio between 194 millimeters (useful) and 254 millimeters (useless) light than conventional lamps. The capillary discharge lamps may include a cone to increase light output. Hollow-core photonic crystal fiber (HCPCF) may also be used.

  15. Exploring Cosmic Origins with CORE: Cosmological Parameters

    CERN Document Server

    Di Valentino, Eleonora; Gerbino, Martina; Poulin, Vivian; Bouchet, François R; Lesgourgues, Julien; Melchiorri, Alessandro; Chluba, Jens; Clesse, Sebastien; Delabrouille, Jacques; Dvorkin, Cora; Forastieri, Francesco; Galli, Silvia; Hooper, Deanna C; Lattanzi, Massimiliano; Martins, Carlos J A P; Salvati, Laura; Cabass, Giovanni; Caputo, Andrea; Giusarma, Elena; Hivon, Eric; Natoli, Paolo; Pagano, Luca; Paradiso, Simone; Rubino-Martin, Jose Alberto; Achucarro, Ana; Ballardini, Mario; Bartolo, Nicola; Baumann, Daniel; Bartlett, James G; de Bernardis, Paolo; Bonaldi, Anna; Bucher, Martin; Cai, Zhen-Yi; De Zotti, Gianfranco; Diego, Josè Maria; Errard, Josquin; Ferraro, Simone; Finelli, Fabio; Genova-Santos, Ricardo T; Gonzalez-Nuevo, Joaquin; Grandis, Sebastian; Greenslade, Josh; Hagstotz, Steffen; Handley, Will; Hindmarsh, Mark; Hernandez-Monteagudo, Carlos; Kiiveri, Kimmo; Kunz, Martin; Lasenby, Anthony; Liguori, Michele; Lopez-Caniego, Marcos; Luzzi, Gemma; Melin, Jean-Baptiste; Mohr, Joseph J; Negrello, Mattia; Paoletti, Daniela; Remazeilles, Mathieu; Ringeval, Christophe; Valiviita, Jussi; Van Tent, Bartjan; Vennin, Vincent; Vittorio, Nicola

    2016-01-01

    We forecast the main cosmological parameter constraints achievable with the CORE space mission which is dedicated to mapping the polarisation of the Cosmic Microwave Background (CMB). CORE was recently submitted in response to ESA's fifth call for medium-sized mission proposals (M5). Here we report the results from our pre-submission study of the impact of various instrumental options, in particular the telescope size and sensitivity level, and review the great, transformative potential of the mission as proposed. Specifically, we assess the impact on a broad range of fundamental parameters of our Universe as a function of the expected CMB characteristics, with other papers in the series focusing on controlling astrophysical and instrumental residual systematics. In this paper, we assume that only a few central CORE frequency channels are usable for our purpose, all others being devoted to the cleaning of astrophysical contaminants. On the theoretical side, we assume LCDM as our general framework and quantify...

  16. HSC90 is required for nascent hepatitis C virus core protein stability in yeast cells.

    Science.gov (United States)

    Kubota, Naoko; Inayoshi, Yasutaka; Satoh, Naoko; Fukuda, Takashi; Iwai, Kenta; Tomoda, Hiroshi; Kohara, Michinori; Kataoka, Kazuhiro; Shimamoto, Akira; Furuichi, Yasuhiro; Nomoto, Akio; Naganuma, Akira; Kuge, Shusuke

    2012-07-30

    Hepatitis C virus core protein (Core) contributes to HCV pathogenicity. Here, we demonstrate that Core impairs growth in budding yeast. We identify HSP90 inhibitors as compounds that reduce intracellular Core protein level and restore yeast growth. Our results suggest that HSC90 (Hsc82) may function in the protection of the nascent Core polypeptide against degradation in yeast and the C-terminal region of Core corresponding to the organelle-interaction domain was responsible for Hsc82-dependent stability. The yeast system may be utilized to select compounds that can direct the C-terminal region to reduce the stability of Core protein.

  17. Measurement of the resonance parameters of the chi(1)(1**3P(1)) and chi(2)(1**3P(2)) states of charmonium formed in antiproton-proton annihilations

    Energy Technology Data Exchange (ETDEWEB)

    Andreotti, M.; Bagnasco, S.; Baldini, W.; Bettoni, D.; Borreani, G.; Buzzo, A.; Calabrese, R.; Cester, R.; Cibinetto, G.; Dalpiaz, P.; Garzoglio, G.; Gollwitzer, K.E.; Graham, M.; Hu, M.; Joffe, D.; Kasper, J.; Lasio, G.; Lo Vetere, M.; Luppi, E.; Macri, M.; Mandelkern, M.; /Fermilab /INFN, Ferrara /Ferrara U. /INFN, Genoa /Genoa U. /UC, Irvine

    2005-03-01

    The authors have studied the {sup 3}P{sub J} ({chi}{sub e}) states of charmonium in formation by antiproton-proton annihilations in experiment E835 at the Fermilab Antiproton Source. The authors report new measurements of the mass, width, and B({chi}{sub cJ} {yields} {bar p}p) x {Lambda}({chi}{sub eJ} {yields} J/{psi} + anything) for the {chi}{sub c1} and {chi}{sub c2} by means of the inclusive reaction {bar p}p {yields} {chi}{sub cJ} {yields} J/{psi} + anything {yields} (e{sup +}e{sup -}) + anything. Using the subsample of events where {chi}{sub cJ} {yields} {gamma} + J/{psi} {yields} {gamma} + (e{sup +}e{sup -}) is fully reconstructed, we derive B({chi}{sub cJ} {yields} {bar p}p) x {Lambda}({chi}{sub cJ} {yields} J/{psi} + {gamma}). They summarize the results of the E760 (updated) and E835 measurements of mass, width and B({chi}{sub cJ} {yields} {bar p}p){Lambda}({chi}{sub cJ} {yields} J/{psi} + {gamma}) (J = 0,1,2) and discuss the significance of these measurements.

  18. Diffusion welding process of submicron aluminium matrix composite Al2O3p/6061 Al%亚微米级Al2O3p/6061Al铝基复合材料扩散焊接工艺

    Institute of Scientific and Technical Information of China (English)

    刘黎明; 高振坤; 董长富; 韩文波

    2004-01-01

    以亚微米级Al2O3p/6061 Al铝基复合材料为对象,研究了直接扩散焊与采用中间层扩散焊两种工艺焊接铝基复合材料的特点、机理,分析了中间层对接头强度的影响规律.结果表明,在铝基复合材料液、固温度区间,存在"临界温度区域",在此温度区域进行直接扩散焊接时,通过液相基体金属的浸润,使得在扩散接合面中增强相-增强相接触转化为增强相-基体-增强相的有机结合,获得高质量焊接接头;进一步研究发现,在扩散接合面上采用合适的基体中间层同样可以将增强相-增强相接触转化为增强相-基体-增强相的有机结合,同时增大"临界温度区域"范围,接头性能更加稳定,接头变形量进一步减小(<2%).

  19. Hanford coring bit temperature monitor development testing results report

    Energy Technology Data Exchange (ETDEWEB)

    Rey, D.

    1995-05-01

    Instrumentation which directly monitors the temperature of a coring bit used to retrieve core samples of high level nuclear waste stored in tanks at Hanford was developed at Sandia National Laboratories. Monitoring the temperature of the coring bit is desired to enhance the safety of the coring operations. A unique application of mature technologies was used to accomplish the measurement. This report documents the results of development testing performed at Sandia to assure the instrumentation will withstand the severe environments present in the waste tanks.

  20. Analytical Chemistry Core Capability Assessment - Preliminary Report

    Energy Technology Data Exchange (ETDEWEB)

    Barr, Mary E. [Los Alamos National Laboratory; Farish, Thomas J. [Los Alamos National Laboratory

    2012-05-16

    The concept of 'core capability' can be nebulous one. Even at a fairly specific level, where core capability equals maintaining essential services, it is highly dependent upon the perspective of the requestor. Samples are submitted to analytical services because the requesters do not have the capability to conduct adequate analyses themselves. Some requests are for general chemical information in support of R and D, process control, or process improvement. Many analyses, however, are part of a product certification package and must comply with higher-level customer quality assurance requirements. So which services are essential to that customer - just those for product certification? Does the customer also (indirectly) need services that support process control and improvement? And what is the timeframe? Capability is often expressed in terms of the currently utilized procedures, and most programmatic customers can only plan a few years out, at best. But should core capability consider the long term where new technologies, aging facilities, and personnel replacements must be considered? These questions, and a multitude of others, explain why attempts to gain long-term consensus on the definition of core capability have consistently failed. This preliminary report will not try to define core capability for any specific program or set of programs. Instead, it will try to address the underlying concerns that drive the desire to determine core capability. Essentially, programmatic customers want to be able to call upon analytical chemistry services to provide all the assays they need, and they don't want to pay for analytical chemistry services they don't currently use (or use infrequently). This report will focus on explaining how the current analytical capabilities and methods evolved to serve a variety of needs with a focus on why some analytes have multiple analytical techniques, and what determines the infrastructure for these analyses. This

  1. miR-625-3p regulates oxaliplatin resistance by targeting MAP2K6-p38 signalling in human colorectal adenocarcinoma cells

    DEFF Research Database (Denmark)

    Rasmussen, Mads Heilskov; Lyskjær, Iben; Jersie-Christensen, Rosa Rakownikow

    2016-01-01

    the signalling networks affected by miR-625-3p. We show that the p38 MAPK activator MAP2K6 is a direct target of miR-625-3p, and, accordingly, is downregulated in non-responder patients of oxaliplatin therapy. miR-625-3p-mediated resistance is reversed by anti-miR-625-3p treatment and ectopic expression of a mi......R-625-3p insensitive MAP2K6 variant. In addition, reduction of p38 signalling by using siRNAs, chemical inhibitors or expression of a dominant-negative MAP2K6 protein induces resistance to oxaliplatin. Transcriptome, proteome and phosphoproteome profiles confirm inactivation of MAP2K6-p38 signalling...... as one likely mechanism of oxaliplatin resistance. Our study shows that miR-625-3p induces oxaliplatin resistance by abrogating MAP2K6-p38-regulated apoptosis and cell cycle control networks, and corroborates the predictive power of miR-625-3p....

  2. The miR-24-3p/p130Cas: a novel axis regulating the migration and invasion of cancer cells.

    Science.gov (United States)

    Kang, Hoin; Rho, Jun Gi; Kim, Chongtae; Tak, Hyosun; Lee, Heejin; Ji, Eunbyul; Ahn, Sojin; Shin, A-Ri; Cho, Hyun-Il; Huh, Yun Hyun; Song, Woo Keun; Kim, Wook; Lee, Eun Kyung

    2017-03-24

    MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression by suppressing translation or facilitating mRNA decay. Differential expression of miRNAs is involved in the pathogenesis of several diseases including cancer. Here, we investigated the role of-miR-24-3p as a downregulated miRNA in metastatic cancer. miR-24-3p was decreased in metastatic cancer and lower expression of miR-24-3p was related to poor survival of cancer patients. Consistently, ectopic expression of miR-24-3p suppressed the cell migration, invasion, and proliferation of MCF7, Hep3B, B16F10, SK-Hep1, and PC-3 cells by directly targeting p130Cas. Stable expression of p130Cas restored miR-24-3p-mediated inhibition of cell migration and invasion. These results suggest that miR-24-3p functions as a tumor suppressor and the miR-24-3p/p130Cas axis is a novel factor of cancer progression by regulating cell migration and invasion.

  3. Emergence of a novel equine-like G3P[8] inter-genogroup reassortant rotavirus strain associated with gastroenteritis in Australian children.

    Science.gov (United States)

    Cowley, Daniel; Donato, Celeste M; Roczo-Farkas, Susie; Kirkwood, Carl D

    2016-02-01

    During 2013, a novel equine-like G3P[8] rotavirus emerged as the dominant strain in Australian children with severe rotavirus gastroenteritis. Full genome analysis demonstrated that the strain was an inter-genogroup reassortant, containing an equine-like G3 VP7, a P[8] VP4 and a genogroup 2 backbone I2-R2-C2-M2-A2-N2-T2-E2-H2. The genome constellation of the equine-like G3P[8] was distinct to Australian and global G3P[8] strains. Phylogenetic analysis demonstrated a genetic relationship to multiple gene segments of Japanese strains RVA/JPN/S13-30/2013/G3P[4] and RVA/Human-wt/JPN/HC12016/2012/G1P[8]. The Australian equine-like G3P[8] strain displayed a distinct VP7 antigenic profile when compared with the previously circulating Australian G3P[8] strains. Identification of similar genes in strains from several geographical regions suggested the equine-like G3P[8] strain was derived by multiple reassortment events between globally co-circulating strains from both human and animal sources. This study reinforces the dynamic nature of rotavirus strains and illustrates the potential for novel human/animal reassortant strains to emerge within the human population.

  4. MicroRNA-143-3p inhibits hyperplastic scar formation by targeting connective tissue growth factor CTGF/CCN2 via the Akt/mTOR pathway.

    Science.gov (United States)

    Mu, Shengzhi; Kang, Bei; Zeng, Weihui; Sun, Yaowen; Yang, Fan

    2016-05-01

    Post-traumatic hypertrophic scar (HS) is a fibrotic disease with excessive extracellular matrix (ECM) production, which is a response to tissue injury by fibroblasts. Although emerging evidence has indicated that miRNA contributes to hypertrophic scarring, the role of miRNA in HS formation remains unclear. In this study, we found that miR-143-3p was markedly downregulated in HS tissues and fibroblasts (HSFs) using qRT-PCR. The expression of connective tissue growth factor (CTGF/CCN2) was upregulated both in HS tissues and HSFs, which is proposed to play a key role in ECM deposition in HS. The protein expression of collagen I (Col I), collagen III (Col III), and α-smooth muscle actin (α-SMA) was obviously inhibited after treatment with miR-143-3p in HSFs. The CCK-8 assay showed that miR-143-3p transfection reduced the proliferation ability of HSFs, and flow cytometry showed that either early or late apoptosis of HSFs was upregulated by miR-143-3p. In addition, the activity of caspase 3 and caspase 9 was increased after miR-143-3p transfection. On the contrary, the miR-143-3p inhibitor was demonstrated to increase cell proliferation and inhibit apoptosis of HSFs. Moreover, miR-143-3p targeted the 3'-UTR of CTGF and caused a significant decrease of CTGF. Western blot demonstrated that Akt/mTOR phosphorylation and the expression of CTGF, Col I, Col III, and α-SMA were inhibited by miR-143-3p, but increased by CTGF overexpression. In conclusion, we found that miR-143-3p inhibits hypertrophic scarring by regulating the proliferation and apoptosis of human HSFs, inhibiting ECM production-associated protein expression by targeting CTGF, and restraining the Akt/mTOR pathway.

  5. miRNA-556-3p promotes tumorigenesis and metastasis by negatively regulating DAB2IP expression in human bladder cancer

    Institute of Scientific and Technical Information of China (English)

    CHEN Feng; SUN Ping; LI Mingqiu; LIU Yueguang; FENG Yukuan; FENG Kejian

    2015-01-01

    Objective:MicroRNAs ( miRNAs) function as key regulator of gene expression and their dereg-ulation play critical roles in tumorigenesis and metastasis of various cancers. The purpose of this study is to identify miRNAs targeting DAB2IP and to determine their expression and function in bladder cancer (BC). Methods and Results:We first predicted candidate miRNAs targeting Disabled homolog 2- interaction protein ( DAB2IP) and then determine their expression and biological function in BC. We showed that miRNA-556-3p directly regulated DAB2 IP expression by binding to DAB2 IP 3 '-UTR and endogenous miRNA-556-3 p expression was significantly up-regulated in clinical samples of BC patients and BC cell lines in comparison to the controls. Conversely, simul-taneous DAB2IP expression in BC tissues and BC cell lines was remarkably down-regulated. Gain or loss function showed that enhanced miRNA-556-3p expression by Lv-miRNA-556-3p transfection promoted proliferation,in-vasion, migration, and colony formation of BC cells, whereas repressed miRNA-556-3p expression by Lv-sh-miRNA-556-3p transfection resulted inan opposite results. Importantly,restored DAB2IP expression by "rescue"assay could attenuate the promotion effect induced by miRNA-556-3p. Further investigation verified that overex-pressed miRNA-556-3p in BC cells not only decreased DAB2IP expression, but also dramatically increased Ras-and pERK1/2 protein expression. In conclusion, our results suggested that DAB2IP was a direct target of miRNA-556-3p, and endogenous miRNA-556-3p expression was reversely correlated with simultaneous DAB2IP expres-sion in BC tissues and cells. Conclusions: MiRNA-556-3p, as a tumor promoter, functioned in tumorigenesis and metastasis of BC via targeting DAB2IP. Moreover, miRNA-556-3p mediated DAB2IP suppression played an oncogenic role by activation of Ras-ERK pathway partially.

  6. Genetic versus Non-Genetic Regulation of miR-103, miR-143 and miR-483-3p Expression in Adipose Tissue and Their Metabolic Implications—A Twin Study

    Directory of Open Access Journals (Sweden)

    Jette Bork-Jensen

    2014-07-01

    Full Text Available Murine models suggest that the microRNAs miR-103 and miR-143 may play central roles in the regulation of subcutaneous adipose tissue (SAT and development of type 2 diabetes (T2D. The microRNA miR-483-3p may reduce adipose tissue expandability and cause ectopic lipid accumulation, insulin resistance and T2D. We aimed to explore the genetic and non-genetic factors that regulate these microRNAs in human SAT, and to investigate their impact on metabolism in humans. Levels of miR-103, miR-143 and miR-483-3p were measured in SAT biopsies from 244 elderly monozygotic and dizygotic twins using real-time PCR. Heritability estimates were calculated and multiple regression analyses were performed to study associations between these microRNAs and measures of metabolism, as well as between these microRNAs and possible regulating factors. We found that increased BMI was associated with increased miR-103 expression levels. In addition, the miR-103 levels were positively associated with 2 h plasma glucose levels and hemoglobin A1c independently of BMI. Heritability estimates for all three microRNAs were low. In conclusion, the expression levels of miR-103, miR-143 and miR-483-3p in adipose tissue are primarily influenced by non-genetic factors, and miR-103 may be involved in the development of adiposity and control of glucose metabolism in humans.

  7. Dielectronic recombination of Fe 3p^q ions: a key ingredient for describing X-ray absorption in active galactic nuclei

    CERN Document Server

    Badnell, N R

    2006-01-01

    We have carried-out multi-configuration Breit-Pauli AUTOSTRUCTURE calculations for the dielectronic recombination (DR) of Fe^{8+} - Fe^{12+} ions. We obtain total DR rate coefficients for the initial ground-level which are an order of magnitude larger than those corresponding to radiative recombination (RR), at temperatures where Fe 3p^q (q=2-6) ions are abundant in photoionized plasmas. The resultant total (DR+RR) rate coefficients are then an order of magnitude larger than those currently in use by photoionized plasma modeling codes such as CLOUDY, ION and XSTAR. These rate coefficients, together with our previous results for q=0 and 1, are critical for determining the ionization balance of the M-shell Fe ions which give rise to the prominent unresolved-transition-array X-ray absorption feature found in the spectrum of many active galactic nuclei. This feature is poorly described by CLOUDY and ION, necessitating an ad hoc modification to the low-temperature DR rate coefficients. Such modifications are no lo...

  8. Effect of microstructural parameters on the yield strength of Al-4%Mg/Al[sub 2]O[sub 3]p composites

    Energy Technology Data Exchange (ETDEWEB)

    Pestes, R.H. (Hewlett-Packard, Camus, WA (United States)); Kamat, S.V.; Hirth, J.P. (Washington State Univ., Pullman, WA (United States). Mechanical and Materials Engineering Dept.)

    1994-04-15

    The quest for materials with high specific strengths and stiffness has been the impetus for the development of metal-matrix composites (MMC's) in recent years. MMC's containing continuous reinforcements possess the highest strengths and stiffnesses in the direction of the reinforcement. However, such composites have poor transverse properties and are extremely expensive for most applications. In contrast, MMC's with particulate reinforcements, while not achieving the strengths and stiffness levels of continuous fiber-reinforced MMC's, exhibit greater isotropy in their properties, Furthermore, they can be processed by conventional means and are much less expensive. The objective of this present investigation was to study the effect of microstructural parameters on the yield strength of Al-4%Mg/Al[sub 2]O[sub 3]p composites containing volume fractions greater than 0.45 and with a bimodal mixture of particle sizes in order to further discriminate among the effects of particulate size and interparticle spacing variables.

  9. Improving green emission of Tb(3)(+) ions in BaO-B2 O3 -P2 O5 glasses by means of Al(3)(+) ions.

    Science.gov (United States)

    Kalpana, T; Gandhi, Y; Sudarsan, V; Piasecki, M; Ravi Kumar, V; Veeraiah, N

    2016-11-01

    BaO-B2 O3 -P2 O5 glasses doped with a fixed concentration of Tb(3)(+) ions and varying concentrations of Al2 O3 were synthesized, and the influence of the Al(3)(+) ion concentration on the luminescence efficiency of the green emission of Tb(3)(+) ions was investigated. The optical absorption, excitation, luminescence spectra and fluorescence decay curves of these glasses were recorded at ambient temperature. The emission spectra of terbium ions when excited at 393 nm exhibited two main groups of bands, corresponding to (5) D3  → (7) Fj (blue region) and (5) D4  → 7Fj (green region). From these spectra, the radiative parameters, viz., spontaneous emission probability A, total emission probability AT , radiative lifetime τ and fluorescent branching ratio β, of different transitions originating from the (5) D4 level of Tb(3)(+) ions were evaluated based on the Judd-Ofelt theory. A clear increase in the quantum efficiency and luminescence of the green emission of Tb(3)(+) ions corresponding to (5) D4  → (7) F5 transition is observed with increases in the concentration of Al2 O3 up to 3.0 mol%. The improvement in emission is attributed to the de-clustering of terbium ions by Al(3)(+) ions and also to the possible admixing of wave functions of opposite parities. Copyright © 2016 John Wiley & Sons, Ltd.

  10. The C(3P) + NH3 Reaction in Interstellar Chemistry. I. Investigation of the Product Formation Channels

    Science.gov (United States)

    Bourgalais, Jérémy; Capron, Michael; Abhinavam Kailasanathan, Ranjith Kumar; Osborn, David L.; Hickson, Kevin M.; Loison, Jean-Christophe; Wakelam, Valentine; Goulay, Fabien; Le Picard, Sébastien D.

    2015-10-01

    The product formation channels of ground state carbon atoms, C(3P), reacting with ammonia, NH3, have been investigated using two complementary experiments and electronic structure calculations. Reaction products are detected in a gas flow tube experiment (330 K, 4 Torr) using tunable vacuum-ultraviolet (VUV) photoionization coupled with time of flight mass spectrometry. Temporal profiles of the species formed and photoionization spectra are used to identify primary products of the C + NH3 reaction. In addition, H-atom formation is monitored by VUV laser induced fluorescence (LIF) from room temperature to 50 K in a supersonic gas flow generated by the Laval nozzle technique. Electronic structure calculations are performed to derive intermediates, transition states, and complexes formed along the reaction coordinate. The combination of photoionization and LIF experiments supported by theoretical calculations indicate that in the temperature and pressure range investigated, the H + H2CN production channel represents 100% of the product yield for this reaction. Kinetics measurements of the title reaction down to 50 K and the effect of the new rate constants on interstellar nitrogen hydride abundances using a model of dense interstellar clouds are reported in Paper II.

  11. Semirigid vibrating rotor target calculation for reaction O(3p)+CH4 →CH3+OH

    Institute of Scientific and Technical Information of China (English)

    LIU; Xinguo; BAI; Lihua; ZHANG; Qinggang

    2004-01-01

    The time-dependent quantum dynamics calculation for reaction O(3p)+CH4→ CH3+OH is made, using of the semirigid vibrating rotor target (SVRT) model and the time-dependent wave packet (TDWP) method. The corresponding reaction probabilities of different initial states are provided. From the calculation of initial rovibrational state j= 0,v= 0, 1, we can see that the excitation of the H-CH3 stretching vibration gives significant enhancement of reaction probability and the reaction threshold decreases dramatically with the enhancement of the vibrating excitation, which indicates that the vibrating energy of reagent molecules contributes a lot to the molecular collision. As for the calculation of reaction probability of state v= 0, j= 0,1,2,3, the results show that the reaction probability rises significantly with the enhancement of rotational quantum number j while the reaction threshold has no changes. The spatial steric effect of the title reaction is studied and analyzed too after the calculation of reaction probability of states j= 5, k= 0-2, n= 0 and j=5, k=2, n=0-2 is made.

  12. Electronic nonadiabatic effects in low temperature radical-radical reactions. I. C(3P) + OH(2Π).

    Science.gov (United States)

    Maergoiz, A I; Nikitin, E E; Troe, J

    2014-07-28

    The formation of collision complexes, as a first step towards reaction, in collisions between two open-electronic shell radicals is treated within an adiabatic channel approach. Adiabatic channel potentials are constructed on the basis of asymptotic electrostatic, induction, dispersion, and exchange interactions, accounting for spin-orbit coupling within the multitude of electronic states arising from the separated reactants. Suitable coupling schemes (such as rotational + electronic) are designed to secure maximum adiabaticity of the channels. The reaction between C((3)P) and OH((2)Π) is treated as a representative example. The results show that the low temperature association rate coefficients in general cannot be represented by results obtained with a single (generally the lowest) potential energy surface of the adduct, asymptotically reaching the lowest fine-structure states of the reactants, and a factor accounting for the thermal population of the latter states. Instead, the influence of non-Born-Oppenheimer couplings within the multitude of electronic states arising during the encounter markedly increases the capture rates. This effect extends up to temperatures of several hundred K.

  13. Remote frequency measurement of the 1S0-3P1 transition in laser cooled 24Mg

    CERN Document Server

    Friebe, J; Wübbena, T; Pape, A; Kelkar, H; Ertmer, W; Terra, O; Sterr, U; Weyers, S; Grosche, G; Schnatz, H; Rasel, E M

    2011-01-01

    We perform Ramsey-Borde spectroscopy on laser cooled magnesium atoms in free fall to measure the 1S0-3P1 intercombination transition frequency. The measured value of 655 659 923 839 730 (48) Hz is consistent with our former atomic beam measurement. We improve upon the fractional accuracy of the previous measurement by more than an order of magnitude to 7 x 10^-14. The magnesium frequency standard was referenced to a fountain clock of the Physikalisch Technische Bundesanstalt (PTB) via a phase-stabilized telecom fiber link and its stability characterized for interrogation times up to 8000 s. Our measurement revealed a new systematic effect due to the movement of atoms across the spectroscopy beams. A remarkable property of this effect is the counterintuitive reduction of residual Doppler shift with increasing resolution. Our theoretical model of the atom-light interaction is in agreement with the observed effect and allows us to quantify its contribution in the uncertainty budget.

  14. Dynamics of interfacial reactions between O({sup 3} P) atoms and long-chain liquid hydrocarbons

    Energy Technology Data Exchange (ETDEWEB)

    Allan, Mhairi [School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom); Bagot, Paul A J [School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom); Koehler, Sven P K [School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom); Reed, Stewart K [Department of Physics and Astronomy, University of Edinburgh, The King' s Buildings, Edinburgh EH9 3JZ (United Kingdom); Westacott, Robin E [School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom); Costen, Matthew L [School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom); McKendrick, Kenneth G [School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom)

    2007-09-15

    Recent progress that has been made towards understanding the dynamics of collisions at the gas-liquid interface is summarized briefly. We describe in this context a promising new approach to the experimental study of gas-liquid interfacial reactions that we have introduced. This is based on laser-photolytic production of reactive gas-phase atoms above the liquid surface and laser-spectroscopic probing of the resulting nascent products. This technique is illustrated for reaction of O({sup 3}P) atoms at the surface of the long-chain liquid hydrocarbon squalane (2,6,10,15,19,23-hexamethyltetracosane). Laser-induced fluorescence detection of the nascent OH has revealed mechanistically diagnostic correlations between its internal and translational energy distributions. Vibrationally excited OH molecules are able to escape the surface. At least two contributions to the product rotational distributions are identified, confirming and extending previous hypotheses of the participation of both direct and trapping-desorption mechanisms. We speculate briefly on future experimental and theoretical developments that might be necessary to address the many currently unanswered mechanistic questions for this, and other, classes of gas-liquid interfacial reaction.

  15. Performance Parameters Analysis of an XD3P Peugeot Engine Using Artificial Neural Networks (ANN) Concept in MATLAB

    Science.gov (United States)

    Rangaswamy, T.; Vidhyashankar, S.; Madhusudan, M.; Bharath Shekar, H. R.

    2015-04-01

    The current trends of engineering follow the basic rule of innovation in mechanical engineering aspects. For the engineers to be efficient, problem solving aspects need to be viewed in a multidimensional perspective. One such methodology implemented is the fusion of technologies from other disciplines in order to solve the problems. This paper mainly deals with the application of Neural Networks in order to analyze the performance parameters of an XD3P Peugeot engine (used in Ministry of Defence). The basic propaganda of the work is divided into two main working stages. In the former stage, experimentation of an IC engine is carried out in order to obtain the primary data. In the latter stage the primary database formed is used to design and implement a predictive neural network in order to analyze the output parameters variation with respect to each other. A mathematical governing equation for the neural network is obtained. The obtained polynomial equation describes the characteristic behavior of the built neural network system. Finally, a comparative study of the results is carried out.

  16. Network nestedness as generalized core-periphery structures

    CERN Document Server

    Lee, Sang Hoon

    2016-01-01

    The concept of nestedness, in particular for ecological and economical networks, has been introduced as a structural characteristic of real interacting systems. We suggest that the nestedness is in fact another way to express a mesoscale network property called the core-periphery structure. With real ecological mutualistic networks and synthetic model networks, we reveal the strong correlation between the nestedness and core-peripheriness, by defining the network-level measures for nestedness and core-peripheriness in case of weighted and bipartite networks. However, at the same time, via more sophisticated null-model analysis, we also discover that the degree (the number of connected neighbors of a node) distribution poses quite severe restrictions on the possible nestedness and core-peripheriness parameter space. Therefore, there must exist structurally interwoven properties in more fundamental levels of network formation, behind this seemingly obvious relation between nestedness and core-periphery structur...

  17. A disappearing heritage: the clinical core of schizophrenia

    DEFF Research Database (Denmark)

    Parnas, Josef

    2011-01-01

    ("schizoidia" and "latent schizophrenia"). The fundamental features are manifest across all domains of consciousness: subjective experience, expression, cognition, affectivity, behavior, and willing. Yet, the specificity of the core was only graspable at a more comprehensive Gestalt-level, variously designated...

  18. Particle-core coupling in 141Nd

    Institute of Scientific and Technical Information of China (English)

    LIU; Minliang(柳敏良); ZHANG; Yuhu(张玉虎); ZHOU; Xiaohong(周小红); GUO; Yingxiang(郭应祥); LIU; Zhong(刘忠); LEI; Xiangguo(雷相国); HE; Jianjun(何建军); ZHENG; Yong(郑勇); LUO; Wanju(罗万居); ZHU; Lihua(竺礼华); WEN; Shuxian(温书贤)

    2003-01-01

    High-spin states in 141Nd have been studied using in-beam γ-ray spectroscopic techniques via the 130Te(16O, 5nγ)141Nd reaction. The level scheme of 141Nd has been extended up to an excitation energy of 7614.5 kev including 12 new γ rays deexciting 15 new levels. According to particle-vibrator coupling and semi-empirical shell model calculations, the level structure of 141Nd can be well interpreted by coupling an h11/2 neutron-hole to the respective excited states in 142Nd core.

  19. Understanding core conductor fabrics

    Energy Technology Data Exchange (ETDEWEB)

    Swenson, D E, E-mail: deswenson@affinity-esd.com [Affinity Static Control Consulting, LLC 2609 Quanah Drive, Round Rock, Texas, 78681 (United States)

    2011-06-23

    ESD Association standard test method ANSI/ESD STM2.1 - Garments (STM2.1), provides electrical resistance test procedures that are applicable for materials and garments that have surface conductive or surface dissipative properties. As has been reported in other papers over the past several years{sup 1} fabrics are now used in many industries for electrostatic control purposes that do not have surface conductive properties and therefore cannot be evaluated using the procedures in STM2.1{sup 2}. A study was conducted to compare surface conductive fabrics with samples of core conductor fibre based fabrics in order to determine differences and similarities with regards to various electrostatic properties. This work will be used to establish a new work item proposal within WG-2, Garments, in the ESD Association Standards Committee in the USA.

  20. Complex coacervate core micelles.

    Science.gov (United States)

    Voets, Ilja K; de Keizer, Arie; Cohen Stuart, Martien A

    2009-01-01

    In this review we present an overview of the literature on the co-assembly of neutral-ionic block, graft, and random copolymers with oppositely charged species in aqueous solution. Oppositely charged species include synthetic (co)polymers of various architectures, biopolymers - such as proteins, enzymes and DNA - multivalent ions, metallic nanoparticles, low molecular weight surfactants, polyelectrolyte block copolymer micelles, metallo-supramolecular polymers, equilibrium polymers, etcetera. The resultant structures are termed complex coacervate core/polyion complex/block ionomer complex/interpolyelectrolyte complex micelles (or vesicles); i.e., in short C3Ms (or C3Vs) and PIC, BIC or IPEC micelles (and vesicles). Formation, structure, dynamics, properties, and function will be discussed. We focus on experimental work; theory and modelling will not be discussed. Recent developments in applications and micelles with heterogeneous coronas are emphasized.

  1. Experimental Determination of the 24Mg I (3s3p)3P2 Lifetime

    DEFF Research Database (Denmark)

    Jensen, Brian Bak; He, Ming; Westergaard, Philip Grabow;

    2011-01-01

    We present the first experimental determination of the electric-dipole forbidden (3s3p)3P2¿(3s2)1S0 (M2) transition rate in 24Mg and compare to state-of-the-art theoretical predictions. Our measurement exploits a magnetic trap isolating the sample from perturbations and a magneto-optical trap...... as an amplifier converting each 3P2¿1S0 decay event into millions of photons readily detected. The transition rate is determined to be (4.87±0.3)×10-4¿¿s-1 corresponding to a 3P2 lifetime of 2050-110+140 sec. This value is in agreement with recent theoretical predictions, and to our knowledge the longest lifetime...

  2. T3P as an efficient cyclodehydration reagent for the one-pot synthesis of 2-amino-1,3,4-oxadiazoles

    Indian Academy of Sciences (India)

    Andivelu Ilangovan; Shanmugasundar Saravanakumar; Siddappa Umesh

    2015-05-01

    A scalable and environmentally friendly one-pot method for the synthesis of 2-amino-1,3,4-oxadiazoles from acylhydrazides and isocyanates has been achieved with propane phosponic anhydride (T3P) acting as cyclodehydrating reagent.

  3. Purification, crystallization and preliminary X-ray diffraction analysis of a soluble variant of the monoglyceride lipase Yju3p from the yeast Saccharomyces cerevisiae

    Energy Technology Data Exchange (ETDEWEB)

    Rengachari, Srinivasan; Aschauer, Philipp; Sturm, Christian; Oberer, Monika, E-mail: m.oberer@uni-graz.at [University of Graz, Humboldtstrasse 50/3, 8010 Graz (Austria)

    2015-01-28

    A soluble variant of the monoglyceride lipase Yju3p was successfully expressed, purified and crystallized. Diffraction data were collected to 2.4 Å resolution. The protein Yju3p is the orthologue of monoglyceride lipases in the yeast Saccharomyces cerevisiae. A soluble variant of this lipase termed s-Yju3p (38.3 kDa) was generated and purified to homogeneity by affinity and size-exclusion chromatography. s-Yju3p was crystallized in a vapour-diffusion setup at 293 K and a complete data set was collected to 2.4 Å resolution. The crystal form was orthorhombic (space group P2{sub 1}2{sub 1}2{sub 1}), with unit-cell parameters a = 77.2, b = 108.6, c = 167.7 Å. The asymmetric unit contained four molecules with a solvent content of 46.4%.

  4. Synthesis and Characterization of Organophosphazene Polyoxotungstate [(N3P3)(SiW11O39H2)3]12-

    Institute of Scientific and Technical Information of China (English)

    Jian GONG; Chang Lu SHAO; Lun Yu QU

    2004-01-01

    A new organophosphazene polyoxotungstate, [(N3P3)(SiW11O39H2)3]12-, has been prepared by reaction of hexachlorocyclotriphosphazene with undecatungstosilicate, and characterized by elemental analysis, infrared spectroscopy, and multinuclear 31P NMR.

  5. C-terminal domain of hepatitis C virus core protein is essential for secretion

    Institute of Scientific and Technical Information of China (English)

    Soo-Ho Choi; Kyu-Jin Park; So-Yeon Kim; Dong-Hwa Choi; Jung-Min Park; Soon B. Hwang

    2005-01-01

    AIM: We have previously demonstrated that hepatitis C virus (HCV) core protein is efficiently released into the culture medium in insect cells. The objective of this study is to characterize the HCV core secretion in insect cells.METHODS: We constructed recombinant baculoviruses expressing various-length of mutant core proteins, expressed these proteins in insect cells, and examined core protein secretion in insect cells.RESULTS: Only wild type core was efficiently released into the culture medium, although the protein expression level of wild type core was lower than those of other mutant core proteins. We found that the shorter form of the core construct expressed the higher level of protein. However, if more than 18 amino acids of the core were truncated at the C-terminus,core proteins were no longer seareted into the culture medium.Membrane flotation data show that the secreted core proteins are associated with the cellular membrane protein, indicating that HCV core is secreted as a membrane complex.CONCLUSION: The C-terminal 18 amino acids of HCV core were crucial for core secretion into the culture media.Since HCV replication occurs on lipid raft membrane structure,these results suggest that HCV may utilize a unique core release mechanism to escape immune surveillance, thereby potentially representing the feature of HCV morphogenesis.

  6. Growth outside the core.

    Science.gov (United States)

    Zook, Chris; Allen, James

    2003-12-01

    Growth in an adjacent market is tougher than it looks; three-quarters of the time, the effort fails. But companies can change those odds dramatically. Results from a five-year study of corporate growth conducted by Bain & Company reveal that adjacency expansion succeeds only when built around strong core businesses that have the potential to become market leaders. And the best place to look for adjacency opportunities is inside a company's strongest customers. The study also found that the most successful companies were able to consistently, profitably outgrow their rivals by developing a formula for pushing out the boundaries of their core businesses in predictable, repeatable ways. Companies use their repeatability formulas to expand into any number of adjacencies. Some companies make repeated geographic moves, as Vodafone has done in expanding from one geographic market to another over the past 13 years, building revenues from $1 billion in 1990 to $48 billion in 2003. Others apply a superior business model to new segments. Dell, for example, has repeatedly adapted its direct-to-customer model to new customer segments and new product categories. In other cases, companies develop hybrid approaches. Nike executed a series of different types of adjacency moves: it expanded into adjacent customer segments, introduced new products, developed new distribution channels, and then moved into adjacent geographic markets. The successful repeaters in the study had two common characteristics. First, they were extraordinarily disciplined, applying rigorous screens before they made an adjacency move. This discipline paid off in the form of learning curve benefits, increased speed, and lower complexity. And second, in almost all cases, they developed their repeatable formulas by studying their customers and their customers' economics very, very carefully.

  7. Application Service Providers (ASP Adoption in Core and Non-Core Functions

    Directory of Open Access Journals (Sweden)

    Aman Y.M. Chan

    2009-10-01

    Full Text Available With the further improvement in internet bandwidth, connection stability and data transmission security, a new wave of Application Service Providers (ASP is on his way. The recent booming on some models such as Software Application as Service (SaaS and On-Demand in 2008, has led to emergence of ASP model in core business functions. The traditional IS outsourcing covers the non-core business functions that are not critical to business performance and competitive advantages. Comparing with traditional IS outsourcing, ASP is a new phenomenon that can be considered as an emerging innovation as it covers both core and non-core business functions. Most of the executives do not comprehend the difference and similarity between traditional IS outsourcing and ASP mode. Hence, we propose to conduct a research so as to identify the determinants (cost benefit, gap in IS capability complementing the company's strategic goal, and trust to ASP's service and security level and moderating factors (management's attitude in ownership & control, and company aggressiveness of ASP adoption decision in both core and non-core business functions.

  8. Epigenetic silencing of miR-490-3p promotes development of an aggressive colorectal cancer phenotype through activation of the Wnt/β-catenin signaling pathway.

    Science.gov (United States)

    Zheng, Kehong; Zhou, Xinying; Yu, Jinlong; Li, Qiang; Wang, Hui; Li, Mingyi; Shao, Ziyun; Zhang, Feifei; Luo, Yuhao; Shen, Zetao; Chen, Fei; Shi, Fujun; Cui, Chunhui; Zhao, Dachuan; Lin, Zhiqun; Zheng, Wei; Zou, Zhaowei; Huang, Zonghai; Zhao, Liang

    2016-06-28

    The Wnt/β-catenin pathway is known to contribute to colorectal cancer (CRC) progression, although little is known about the contribution of β-catenin on this process. We investigated the role of miR-490-3p, which was recently reported to suppress tumorigenesis through its effect on Wnt/β-catenin signaling. We found that hypermethylation of the miR-490-3p promoter down-regulates miR-490-3p expression in CRC tissue. Gain- and loss-of-function assays in vitro and in vivo reveal that miR-490-3p suppresses cancer cell proliferation by inducing apoptosis and inhibits cell invasiveness by repressing the initiation of epithelial-to-mesenchymal transition (EMT), a key mechanism in cancer cell invasiveness and metastasis. The frequently rearranged in advanced T-cell lymphomas (FRAT1) protein was identified as a direct target of miR-490-3p and contributes to its tumor-suppressing effects. miR-490-3p appears to have an inhibitory effect on β-catenin expression in nuclear fractions of CRC cells, whereas FRAT1 expression is associated with the accumulation of β-catenin in the nucleus of cells, which could be weakened by transfection with miR-490-3p. Our findings suggest that the miR-490-3p/FRAT1/β-catenin axis is important in CRC progression and provides new insight into the molecular mechanisms underlying CRC. They may help to confirm the pathway driving CRC aggressiveness and serve for the development of a novel miRNA-targeting anticancer therapy.

  9. miR-181b-3p promotes epithelial-mesenchymal transition in breast cancer cells through Snail stabilization by directly targeting YWHAG.

    Science.gov (United States)

    Yoo, Je-Ok; Kwak, Seo-Young; An, Hyun-Ju; Bae, In-Hwa; Park, Myung-Jin; Han, Young-Hoon

    2016-07-01

    Epithelial-mesenchymal transition (EMT) is essential for increased invasion and metastasis during cancer progression. Among the candidate EMT-regulating microRNAs that we previously identified, miR-181b-3p was found to induce EMT in MCF7 breast cancer cells, as indicated by an EMT-characteristic morphological change, increased invasiveness, and altered expression of an EMT marker. Transfection with a miR-181b-3p inhibitor reduced the expression of mesenchymal markers and the migration and invasion of highly invasive breast cancer cells. miR-181b-3p induced the upregulation of Snail, a master EMT inducer and transcriptional repressor of E-cadherin, through protein stabilization. YWHAG was identified as a direct target of miR-181b-3p, downregulation of which induced Snail stabilization and EMT phenotypes. Ectopic expression of YWHAG abrogated the effect of miR-181b-3p, including Snail stabilization and the promotion of invasion. In situ hybridization and immunohistochemical analyses indicated that YWHAG expression was inversely correlated with the expression of miR-181b-3p and Snail in human breast cancer tissues. Furthermore, transfection with miR-181b-3p increased the frequency of metastatic nodule formation in the lungs of mice in experimental metastasis assays using MDA-MB-231 cells. Taken together, our data suggest that miR-181b-3p functions as a metastasis activator by promoting Snail-induced EMT, and may therefore be a therapeutic target in metastatic cancers.

  10. p53 induces miR199a-3p to suppress SOCS7 for STAT3 activation and renal fibrosis in UUO

    Science.gov (United States)

    Yang, Ruhao; Xu, Xuan; Li, Huiling; Chen, Jinwen; Xiang, Xudong; Dong, Zheng; Zhang, Dongshan

    2017-01-01

    The role of p53 in renal fibrosis has recently been suggested, however, its function remains controversial and the underlying mechanism is unclear. Here, we show that pharmacological and genetic blockade of p53 attenuated renal interstitial fibrosis, apoptosis, and inflammation in mice with unilateral urethral obstruction (UUO). Interestingly, p53 blockade was associated with the suppression of miR-215-5p, miR-199a-5p&3p, and STAT3. In cultured human kidney tubular epithelial cells (HK-2), TGF-β1 treatment induced fibrotic changes, including collagen I and vimentin expression, being associated with p53 accumulation, p53 Ser15 phosphorylation, and miR-199a-3p expression. Inhibition of p53 by pifithrin-α blocked STAT3 activation and the expression of miR-199a-3p, collagen I, and vimentin during TGF-β1 treatment. Over-expression of miR-199a-3p increased TGFβ1-induced collagen I and vimentin expression and restored SOCS7 expression. Furthermore, SOCS7 was identified as a target gene of miR-199a-3p, and silencing of SOCS7 promoted STAT3 activation. ChIp analyses indicated the binding of p53 to the promoter region of miR-199a-3p. Consistently, kidney biopsies from patients with IgA nephropathy and diabetic nephropathy exhibited substantial activation of p53 and STAT3, decreased expression of SOCS7, and increase in profibrotic proteins and miR-199a-3p. Together, these results demonstrate the novel p53/miR-199a-3p/SOCS7/STAT3 pathway in renal interstitial fibrosis. PMID:28240316

  11. miR-659-3p is involved in the regulation of the chemotherapy response of colorectal cancer via modulating the expression of SPHK1

    Science.gov (United States)

    Li, Shuyuan; Fang, Ying; Qin, Hai; Fu, Wenzheng; Zhang, Xipeng

    2016-01-01

    Colorectal cancer (CRC) is one of most prevalent malignant diseases worldwide. Metastasis and chemo-resistance are the two prominent death-related factors of CRCs. Thus, it is urgent to understand the mechanism responsible for the chemo-resistant properties of CRC and develop new therapeutic methods. Here, we found that the expression of miR-659-3p was significantly reduced in cisplatin (CDDP)-resistant HT29 and LOVO colorectal cancer cells and in CDDP-resistant clinical colorectal cancer samples compared with respective CDDP-sensitive counterparts. Sphingosine kinase 1 (SPHK1) is a direct target of miR-659-3p in colorectal cancer cells, and it is negatively regulated by miR-659-3p. We found that anti-miR-659-3p could increase the IC50 of CDDP in parental HT29 and LOVO colorectal cancer cells; additionally, miR-659-3p mimics decreased the IC50 of CDDP in HT29/CDDP and LOVO/CDDP colorectal cancer cells. Furthermore, we showed that the miR-659-3p/SPHK1 pathway was involved in the regulation of chemotherapy responses of colorectal cancer cells in vivo. In all, our findings suggest a new mechanism involved in the regulation of the chemotherapy response of CRC and might provide new targets for CRC prevention and treatment. PMID:27725903

  12. Design of a Robust, Computation-Efficient and Secure 3P-EKE Protocol using Analogous Message Transmission

    Directory of Open Access Journals (Sweden)

    Archana Raghuvamshi

    2016-05-01

    Full Text Available In this modern era of digital communication even a trivial task needs to be performed over internet which is not secure. Many cryptographic algorithms existed to provide security which facilitates secure communication through internet. As these algorithms need a secret session key, it is required to interchange this key in a secure way. In two-party communication, two clients initially share a low random (entropy password through a secure channel to establish a secret session key. But this paradigm necessitates high maintenance of passwords, since each communicating pair requires separate passwords to establish a secure session key. In three-party communication network, each communication party shares a password with the trusted third-party (server to exchange a secret session key. The beauty of this setting is that, even a server does not know the session key. The Password Authenticated Encrypted Key Exchange (PA-EKE protocols have attracted a lot of curiosity to authors to propose various two-party and three-party PA-EKE protocols. Security flaws in various protocols proposed by Chang-Chang, Yoon-Yoo, PSRJ and Raj et al. inspired to design a robust, computationally efficient and highly secure protocol. This paper is an attempt to propose a secure and novel Password Authenticated 3P-EKE protocol using XOR operations and analogous (parallel message transmission. The proposed protocol is easy to design and more secured against all types of attacks like password guessing, replay, pre-play, server spoofing etc. which made this protocol special.

  13. Regional hyperthermia in conjunction with definitive radiotherapy against recurrent or locally advanced prostate cancer T3pNoMo

    Energy Technology Data Exchange (ETDEWEB)

    Tilly, W.; Gellermann, J.; Graf, R.; Felix, R.; Wust, P. [Dept. of Radiation Medicine, Charite Medical School, Berlin (Germany); Hildebrandt, B. [Dept. of Internal Medicine - Hematology and Oncology, Charite Medical School, Berlin (Germany); Weissbach, L. [Dept. of Urology, Urban Hospital, Berlin (Germany); Budach, V. [Dept. of Radiation Oncology, Charite Medical School, Berlin (Germany)

    2005-01-01

    Background and purpose: since long-term results of the standard treatment of locally advanced or recurrent prostatic carcinoma are unsatisfactory, the role for additional regional hyperthermia was evaluated in a phase I/II study. Patients and methods: from 08/1996 to 03/2000, 22 patients were treated by a standard irradiation regimen (68.4 Gy) in combination with regional hyperthermia (weekly, five to six times), and five of 22 patients received short-term (neoadjuvant) hormonal treatment. Of these, 15 patients had primary prostatic carcinoma T3 pNO MO and seven a histologically confirmed local recurrence after radical prostatectomy. Feasibility of hyperthermia, and acute/late toxicity as well as long-term follow-up (prostate-specific antigen [PSA] control, overall survival) were analyzed. Clinical endpoints were correlated with thermal parameters. Results: mean maximum temperatures along the urethra of 41.4 C (41.0 C for the recurrences), and mean T{sub 90} values of 40.7 C could be achieved. Severe acute toxicity of grade 3 occurred at the rectum in three, at the urethra in four, at the intestine in one, and a burn induced by hyperthermia in one of 22 patients. Late toxicity was only observed rectally in one patient (grade 3) and at the urethra in two patients (grade 2). There was no correlation between thermal parameters and any toxicity. The survival curves showed a PSA control for primary prostatic carcinoma > 50% after 6 years, but no long-term PSA control for the recurrences. Overall survival after 6 years was 95% for primary carcinoma, and 60% for the recurrences. There was a clear correlation between higher temperatures or thermal doses with long-term PSA control. Conclusion: regional hyperthermia might be a low-toxicity approach to increase PSA control of common treatment schedules. Further evaluation, in particular employing improved hyperthermia technology, is worthwhile. (orig.)

  14. Advances in core drilling technology

    Science.gov (United States)

    Holdsworth, G.

    Some notable technical advances in drill design were reported at the meeting, held in Canada August 30-September 1, 1982, at the University of Calgary. Chief amongst these was a battery powered, computer assisted electromechanical core drill which has recently been used by the Danes in Greenland to continuously core to the base of the ice sheet at 2038 m. This is the deepest coring operation so far on the Greenland ice sheet. (The record for deep glacier drilling is held by the U.S. Army Cold Regions Research and Engineering Laboratory for the continuous coring through 2164 m of ice to bedrock at Byrd Station, Antarctica, in 1968). In early 1982, a current Soviet core drilling operation was reported to be at a depth of 2000 m at Vostok station, Antarctica, where the total ice thickness is about 4000 m; the goal of core drilling the entire ice thickness there could be achieved before the end of 1983.

  15. Actin-binding protein regulation by microRNAs as a novel microbial strategy to modulate phagocytosis by host cells: the case of N-Wasp and miR-142-3p

    Directory of Open Access Journals (Sweden)

    Paulo eBettencourt

    2013-06-01

    Full Text Available Mycobacterium tuberculosis (Mtb is a successful intracellular pathogen that thrives in macrophages (Mφs. There is a need to better understand how Mtb hijacks cellular processes like phagolysosome biogenesis, a classical pathogenesis determinant. A central feature to this microbial strategy is the manipulation of actin in Mφs. Here, we examine the role of microRNAs (miRNAs as a potential mechanism in the regulation of actin-mediated events leading to phagocytosis in the context of mycobacteria infection. Given that non-virulent Mycobacterium smegmatis also controls actin filamentation to prolong its instracellular survival inside host cells, our approach was to perform a global transcriptomic approach to assess the modulation of miRNAs upon M. smegmatis infection of the murine Mφ cell line, J774A.1. This approach yielded miR-142-3p as a top candidate to be involved in the regulation of actin dynamics required in phagocytosis. We unequivocally demonstrate miR-142-3p targets N-Wasp, an actin-binding protein required during microbial challenge. A gain-of-function approach for miR-142-3p revealed a down-regulation of N-Wasp expression accompanied by a decrease of mycobacteria intake, while a loss-of-function approach yielded the reciprocal increase of the phagocytosis process. Equally important, we show Mtb induces the early expression of miR-142-3p and down-regulates N-Wasp protein levels in both the murine J774A.1 cell line and primary human Mφs. As proof of principle, the partial siRNA-mediated knock down of N-Wasp resulted in a decrease of Mtb intake by human Mφs, reflected in lower levels of colony-forming units counts over time. We therefore propose the modulation of miRNAs as a novel strategy in mycobacterial infection to control factors involved in actin filamentation and other early events of phagolysosome biogenesis, such as the case presented here for miR-142-3p influencing N-Wasp activity during the phagocytosis process.

  16. Biochemistry Instrumentation Core Technology Center

    Data.gov (United States)

    Federal Laboratory Consortium — The UCLA-DOE Biochemistry Instrumentation Core Facility provides the UCLA biochemistry community with easy access to sophisticated instrumentation for a wide variety...

  17. PWR core stablity aganst xenon-induced spatial power oscillation

    Energy Technology Data Exchange (ETDEWEB)

    Moon, H.J.; Han, K.I. (Korea Advanced Energy Research Inst., Seoul (Republic of Korea))

    1982-06-01

    Stability of a PWR core against xenon-induced axial power oscillation is studied using one-dimensional xenon transient analysis code, DD1D, that has been developed and verified at KAERI. Analyzed by DD1D utilizing the Kori Unit 1 design and operating data is the sensitivity of axial stability in a PWR core to the changes in core physical parameters including core power level, moderator temperature coefficient, core inlet temperature, doppler power coefficient and core average burnup. Through the sensitivity study the Kori Unit 1 core is found to be stable against axial xenon oscillation at the beginning of cycle 1. But, it becomes less stable as burnup progresses, and unstable at the end of cycle. Such a decrease in stability is mainly due to combined effect of changes in axial power distribution, moderator temperature coefficient and doppler power coefficient as core burnup progresses. It is concluded from the stability analysis of the Kori Unit 1 core that design of a large PWR with high power density and increased dimension can not avoid xenon-induced axial power instabilites to some extents, especially at the end of cycle.

  18. Measurement of noise associated with model transformer cores

    Energy Technology Data Exchange (ETDEWEB)

    Snell, David [Cogent Power Ltd., Development and Market Research, Orb Electrical Steels, Corporation Road, Newport, South Wales NP19 OXT (United Kingdom)], E-mail: Dave.snell@cogent-power.com

    2008-10-15

    The performance of a transformer core may be considered in terms of power loss and by the noise generated by the core, both of which should be minimised. This paper discusses the setting up of a suitable system for evaluation of noise in a large model transformer core (500 kV A) and issues associated with noise measurement. The equivalent continuous sound pressure level (LAeq) was used as a measure of the A-weighted sound level and measurements were made in the range 16 Hz-25 kHz for various step lap core configurations. The selection of optimum sound insulation materials between core and ground support and for enclosing the transformer is essential for minimisation of background noise. Core clamping pressure must be optimised in order to minimise noise. The use of two laminations per layer instead of one leads to an increase in noise arising from the core. Provided care is taken in building the core, good reproducibility of results can be obtained for analysis.

  19. miR-206-3p在小鼠皮肤中的表达及其作用%Expression and distribution of miR-206-3p in mouse skin and its potential roles

    Institute of Scientific and Technical Information of China (English)

    穆原; 周红; 李文艳; 李耀武

    2013-01-01

    Objective To investigate the expression and distribution of miR-206-3p and its target gene Bdnf (brain-derived neurotrophic factor) in mouse skin and the potential roles during the development of skin.Methods The full-thickness back skins of BALB/c mice on the 13.5 dpc (day post coition),15.5 dpc,17.5 dpc,1 dpp (day post partum),4 dpp and 16 weeks were collected respectively.The expression levels of miR-206-3p and BdnfmRNA were measured by real time RT-PCR,and BDNF protein was detected by western blot.The distributions of miR-206-3p and BDNF in tissue were profiled by in situ hybridization (ISH) and immunohistochemistry (IHC) respectively.Results The expression level of miR-206-3p increased gradually until 17.5 dpc and subsequently receded to the similar level of the 13.5 dpc during adult stage.The expression of BDNF protein exhibited in the opposite manner of miR-206-3p but BdnfmRNA remained stable from the 17.5 dpc until adult stage.ISH results showed that the distribution of miR-206-3p was gradually regionalized during the development of skin in the postnatal days and mainly located in the suprabasal layer and periphery of hair follicles,which was adjacent to the regions of BDNF expression at the same stage in a nonoverlapping manner.Conclusion The miR-206-3p may be involved in neurodevelopment of mouse skin through the post-transcriptional regulation of Bdnf.%目的 对小鼠皮肤中miR-206-3p及其靶基因脑源性神经营养因子(Bdnf)进行表达与定位检测,以探讨其在皮肤发育过程中的可能作用.方法 手术剪取13.5 dpc(交配后天数)、15.5 dpc、17.5 dpc胎鼠,1 dpp(出生后天数)、4 dpp乳鼠及16周龄雄鼠的背部全层皮肤,用实时荧光定量RT-PCR检测miR-206-3p与Bdnf mRNA的表达水平,western blot检测BDNF蛋白质表达水平,原位杂交与免疫组化分别检测miR-206-3p与BDNF的组织定位.结果 皮肤中miR-206-3p在胚胎期持续升高,到17.5 dpc后逐渐降低,于成年鼠时回复至13.5 dpc时

  20. Waves in the core and mechanical core-mantle interactions

    DEFF Research Database (Denmark)

    Jault, D.; Finlay, Chris

    2015-01-01

    the motions in the direction parallel to the Earth'srotation axis. This property accounts for the signicance of the core-mantle topography.In addition, the stiening of the uid in the direction parallel to the rotation axis gives riseto a magnetic diusion layer attached to the core-mantle boundary, which would...