Sample records for 3c clamped downhole

  1. Development of a 400 Level 3C Clamped Downhole Seismic Receiver Array for 3D Borehole Seismic Imaging of Gas Reservoirs

    Bjorn N. P. Paulsson


    Borehole seismology is the highest resolution geophysical imaging technique available today to the oil and gas industry for characterization and monitoring of oil and gas reservoirs. However, the industry's ability to perform high resolution 3D imaging of deep and complex gas reservoirs using borehole seismology has been hampered by the lack of acquisition technology necessary to record large volumes of high frequency, high signal-to-noise-ratio borehole seismic data. This project took aim at this shortcoming by developing a 400 level 3C clamped downhole seismic receiver array, and accompanying software, for borehole seismic 3D imaging. This large borehole seismic array has removed the technical acquisition barrier for recording the data volumes necessary to do high resolution 3D VSP and 3D cross-well seismic imaging. Massive 3D VSP{reg_sign} and long range Cross-Well Seismology (CWS) are two of the borehole seismic techniques that promise to take the gas industry to the next level in their quest for higher resolution images of deep and complex oil and gas reservoirs. Today only a fraction of the oil or gas in place is produced when reservoirs are considered depleted. This is primarily due to our lack of understanding of detailed compartmentalization of oil and gas reservoirs. In this project, we developed a 400 level 3C borehole seismic receiver array that allows for economic use of 3D borehole seismic imaging for reservoir characterization and monitoring. This new array has significantly increased the efficiency of recording large data volumes at sufficiently dense spatial sampling to resolve reservoir complexities. The receiver pods have been fabricated and tested to withstand high temperature (200 C/400 F) and high pressure (25,000 psi), so that they can operate in wells up to 7,620 meters (25,000 feet) deep. The receiver array is deployed on standard production or drill tubing. In combination with 3C surface seismic or 3C borehole seismic sources, the 400

  2. Distributed downhole drilling network

    Hall, David R.; Hall, Jr., H. Tracy; Fox, Joe; Pixton, David S.


    A high-speed downhole network providing real-time data from downhole components of a drilling strings includes a bottom-hole node interfacing to a bottom-hole assembly located proximate the bottom end of a drill string. A top-hole node is connected proximate the top end of the drill string. One or several intermediate nodes are located along the drill string between the bottom-hole node and the top-hole node. The intermediate nodes are configured to receive and transmit data packets transmitted between the bottom-hole node and the top-hole node. A communications link, integrated into the drill string, is used to operably connect the bottom-hole node, the intermediate nodes, and the top-hole node. In selected embodiments, a personal or other computer may be connected to the top-hole node, to analyze data received from the intermediate and bottom-hole nodes.

  3. Downhole Fluid Analyzer Development

    Bill Turner


    A novel fiber optic downhole fluid analyzer has been developed for operation in production wells. This device will allow real-time determination of the oil, gas and water fractions of fluids from different zones in a multizone or multilateral completion environment. The device uses near infrared spectroscopy and induced fluorescence measurement to unambiguously determine the oil, water and gas concentrations at all but the highest water cuts. The only downhole components of the system are the fiber optic cable and windows. All of the active components--light sources, sensors, detection electronics and software--will be located at the surface, and will be able to operate multiple downhole probes. Laboratory testing has demonstrated that the sensor can accurately determine oil, water and gas fractions with a less than 5 percent standard error. Once installed in an intelligent completion, this sensor will give the operating company timely information about the fluids arising from various zones or multilaterals in a complex completion pattern, allowing informed decisions to be made on controlling production. The research and development tasks are discussed along with a market analysis.

  4. Downhole pulse tube refrigerators

    Swift, G.; Gardner, D. [Los Alamos National Lab., NM (United States). Condensed Matter and Thermal Physics Group


    This report summarizes a preliminary design study to explore the plausibility of using pulse tube refrigeration to cool instruments in a hot down-hole environment. The original motivation was to maintain Dave Reagor`s high-temperature superconducting electronics at 75 K, but the study has evolved to include three target design criteria: cooling at 30 C in a 300 C environment, cooling at 75 K in a 50 C environment, cooling at both 75 K and 30 C in a 250 C environment. These specific temperatures were chosen arbitrarily, as representative of what is possible. The primary goals are low cost, reliability, and small package diameter. Pulse-tube refrigeration is a rapidly growing sub-field of cryogenic refrigeration. The pulse tube refrigerator has recently become the simplest, cheapest, most rugged and reliable low-power cryocooler. The authors expect this technology will be applicable downhole because of the ratio of hot to cold temperatures (in absolute units, such as Kelvin) of interest in deep drilling is comparable to the ratios routinely achieved with cryogenic pulse-tube refrigerators.

  5. Battery switch for downhole tools

    Boling, Brian E.


    An electrical circuit for a downhole tool may include a battery, a load electrically connected to the battery, and at least one switch electrically connected in series with the battery and to the load. The at least one switch may be configured to close when a tool temperature exceeds a selected temperature.

  6. A microelectromechanical system digital 3C array seismic cone penetrometer

    Ghose, R.


    A digital 3C array seismic cone penetrometer has been developed for multidisciplinary geophysical and geotechnical applications. Seven digital triaxial microelectromechanical system accelerometers are installed at 0.25-m intervals to make a 1.5-m-long downhole seismic array. The accelerometers have

  7. Photovoltaic panel clamp

    Mittan, Margaret Birmingham; Miros, Robert H. J.; Brown, Malcolm P.; Stancel, Robert


    A photovoltaic panel clamp includes an upper and lower section. The interface between the assembled clamp halves and the module edge is filled by a flexible gasket material, such as EPDM rubber. The gasket preferably has small, finger like protrusions that allow for easy insertion onto the module edge while being reversed makes it more difficult to remove them from the module once installed. The clamp includes mounting posts or an integral axle to engage a bracket. The clamp also may include a locking tongue to secure the clamp to a bracket.

  8. Corrosion reference for geothermal downhole materials selection

    Ellis, P.F. II, Smith, C.C.; Keeney, R.C.; Kirk, D.K.; Conover, M.F.


    Geothermal downhole conditions that may affect the performance and reliability of selected materials and components used in the drilling, completion, logging, and production of geothermal wells are reviewed. The results of specific research and development efforts aimed at improvement of materials and components for downhole contact with the hostile physicochemical conditions of the geothermal reservoir are discussed. Materials and components covered are tubular goods, stainless steels and non-ferrous metals for high-temperature downhole service, cements for high-temperature geothermal wells, high-temperature elastomers, drilling and completion tools, logging tools, and downhole pumps. (MHR)

  9. Radial wedge flange clamp

    Smith, Karl H.


    A radial wedge flange clamp comprising a pair of flanges each comprising a plurality of peripheral flat wedge facets having flat wedge surfaces and opposed and mating flat surfaces attached to or otherwise engaged with two elements to be joined and including a series of generally U-shaped wedge clamps each having flat wedge interior surfaces and engaging one pair of said peripheral flat wedge facets. Each of said generally U-shaped wedge clamps has in its opposing extremities apertures for the tangential insertion of bolts to apply uniform radial force to said wedge clamps when assembled about said wedge segments.

  10. 275 C Downhole Microcomputer System

    Chris Hutchens; Hooi Miin Soo


    An HC11 controller IC and along with serial SRAM and ROM support ICs chip set were developed to support a data acquisition and control for extreme temperature/harsh environment conditions greater than 275 C. The 68HC11 microprocessor is widely used in well logging tools for control, data acquisition, and signal processing applications and was the logical choice for a downhole controller. This extreme temperature version of the 68HC11 enables new high temperature designs and additionally allows 68HC11-based well logging tools and MWD tools to be upgraded for high temperature operation in deep gas reservoirs, The microcomputer chip consists of the microprocessor ALU, a small boot ROM, 4 kbyte data RAM, counter/timer unit, serial peripheral interface (SPI), asynchronous serial interface (SCI), and the A, B, C, and D parallel ports. The chip is code compatible with the single chip mode commercial 68HC11 except for the absence of the analog to digital converter system. To avoid mask programmed internal ROM, a boot program is used to load the microcomputer program from an external mask SPI ROM. A SPI RAM IC completes the chip set and allows data RAM to be added in 4 kbyte increments. The HC11 controller IC chip set is implemented in the Peregrine Semiconductor 0.5 micron Silicon-on-Sapphire (SOS) process using a custom high temperature cell library developed at Oklahoma State University. Yield data is presented for all, the HC11, SPI-RAM and ROM. The lessons learned in this project were extended to the successful development of two high temperature versions of the LEON3 and a companion 8 Kbyte SRAM, a 200 C version for the Navy and a 275 C version for the gas industry.

  11. Downhole drilling spotting fluid composition and method

    Hayes, J.R.; Campbell, G.J.


    This paper discusses a spotting fluid concentrate composition suitable for use in downhole drilling operations in a pill for releasing periodically stuck drill string. It comprises glycerophosphoric acid ester; a polyacyloxy polycarboxylic acid ester of monoglycerides, diglycerides and mixtures thereof; an optional viscosifying agent for increasing the viscosity of the dispersion; and an optional sealing agent for preventing diffusion of water into the downhole formation. This patent also describes a method for lubricating a downhole well drilling operation. It comprises mixing a spotting fluid concentrate composition comprising glycerophosphoric acid ester, a polyacyloxy polycarboxylic acid ester of monoglycerides, diglycerides and mixtures thereof, an optional viscosifying agent and an optional sealing agent with drilling mud; and circulating the mud mixture through the well.

  12. Downhole elemental analysis with LIBS

    Moreschini, P.; Zacny, K.; Rickman, D.


    top of the downhole probe allows radial scanning of the borehole. Analysis of iron and titanium in lunar simulant with LIBS was performed in air using the method of standard addition. The results for lunar simulant NU-LHT-2M show a value for the concentration of iron ranging between 2.29% and 3.05% depending on the atomic line selected. The accepted value for the sample analyzed is 2.83%, showing the capability for the system in development to provide qualitative and semi-quantitative analysis in real-time.

  13. Downhole dehydration - status report and implementation study; Downhole Dehydration - Statusbericht und Umsetzungsstudie

    Jacobs, D.; Schmidt, D. [BEB Erdgas und Erdoel GmbH, Hannover (Germany)


    Downhole dehydration, i.e. in-situ separation of water and oil, is an interesting new technology. The contribution describes the technology and the results of a field experiment. (orig.) [Deutsch] Die Tail-End Foerderphase in der deutschen Erdoelproduktion, welche durch hohe Wasserhebekosten gekennzeichnet ist, erfordert zur Aufrechterhaltung der Wirtschaftlichkeit neue Gedankenansaetze. Ein aus wirtschaftlicher und technischer Sicht reizvoller Optimierungsgedanke ist die untertaegige Wasser/Oel Separation, auch Downhole Dehydration genannt. Unter Downhole Dehydration (DHD) versteht man also die untertaegige (teilweise) Separation des Lagerstaettenwassers vom Nassoel, kurz nachdem das Gemisch untertaegig in das Bohrloch eingetreten ist. Dabei wird das abgetrennte Lagerstaettenwasser untertage in einen geeigneten Horizont unmittelbar wieder injiziert und das Oel (wie bei der konventionellen Foerderung) zutage gepumpt, mit dem Ziel der Nutzung der daraus resultierenden Kosten- und Investitionsersparnis. Ziel dieses Vortrages ist es, einen kurzen Einblick in die erstmalig in Europa angewandte Technik zu geben und von den Erfahrungen des praktizierten Feldversuches zu berichten. (orig.)

  14. Development of a downhole seismic source with controlled waveform; Hakei seigyogata kochu shingen no kaihatsu

    Kuroda, T.; Ikawa, T. [Japex Jeoscience Institute, Tokyo (Japan); Sato, T. [Meiho Engineering Co. Ltd., Tokyo (Japan); Kakuma, H. [Akashi Corp., Tokyo (Japan); Onuma, H. [Engineering Advancement Association of Japan, Tokyo (Japan)


    A downhole seismic source which can output continuous waves having arbitrary waveforms was developed. The development was targeted to make tomographic exploration purposed to evaluate geological properties of a ground bed before and after constructing a building in a ground several hundred meters deep from the ground surface. The source is considered to be used in an environment consisting of soft rocks or more robust rocks and having no casing. It can be used in a well hole having a diameter of 100 mm, is capable of measuring P and S waves in a distance between well holes of up to 100 m, can be used at a depth of up to 500 m, and can output waveforms having seismic source spectra of up to 1000 Hz. An oscillation actuator using laminated piezo-electric elements was used for the oscillation element. The seismic source consists of a hydraulic device to clamp the equipment onto hole walls, piezo-electric elements as the oscillation element, and an inertia weight for applying vibration from above and below. To make an oscillation, the main body is first clamped on the hole wall. For horizontal oscillation, the piezo-electric elements contained in a clamping device provide the horizontal oscillation. For vertical oscillation, the piezo-electric elements placed below the main body oscillates the inertia weight. The initially targeted specifications have been achieved. 3 refs., 4 figs., 1 tab.

  15. Measurement strategies for downhole multiphase metering

    Hammer, Erling A.; Johansen, Geir Anton; Tollefsen, Jarle; Aabro, Eirik [Bergen Univ.(Norway)


    There will be an increasing demand for multiphase subsea and downhole meters in the future. Both at the sea bottom and downhole the flow regimes in the production pipes or in the manifolds at the templates, may differ from the ideal homogeneous mixture. Further, in line mixers should be avoided to reduce pressure drops and maintenance costs. The next generation multiphase meters will therefore call for flow regime independent and non-intrusive sensor systems. Since all sensor principles used in multiphase flowmeters today are highly dependent on the distribution of the components in the mixture, and thus make the measurement range limited, multi-sensor principles may be the solution to obtain better accuracy for larger ranges of component fractions and applications. Both the capacitance-, conductance-, microwave- and gamma-principles can be used in multi-sensor arrangement to provide cross-sectional information about the component distribution. Hence, the meter can be used at all types of flow regimes and at any position without mixers or separators. (author)

  16. Downhole tool with replaceable tool sleeve sections

    Case, W. A.


    A downhole tool for insertion in a drill stem includes elongated cylindrical half sleeve tool sections adapted to be non-rotatably supported on an elongated cylindrical body. The tool sections are mountable on and removable from the body without disconnecting either end of the tool from a drill stem. The half sleeve tool sections are provided with tapered axially extending flanges on their opposite ends which fit in corresponding tapered recesses formed on the tool body and the tool sections are retained on the body by a locknut threadedly engaged with the body and engageable with an axially movable retaining collar. The tool sections may be drivably engaged with axial keys formed on the body or the tool sections may be formed with flat surfaces on the sleeve inner sides cooperable with complementary flat surfaces formed on a reduced diameter portion of the body around which the tool sections are mounted.

  17. Robotic Partial Nephrectomy Using Robotic Bulldog Clamps


    Background and Objectives: The need for a skilled assistant to perform hilar clamping during robotic partial nephrectomy is a potential limitation of the technique. We describe our experience using robotic bulldog clamps applied by the console surgeon for hilar clamping. Methods: A total of 60 consecutive patients underwent robotic partial nephrectomy, 30 using laparoscopic bulldog clamps applied by the assistant and 30 using robotic bulldog clamps applied with the robotic Prograsp instrument...

  18. Automated planar patch-clamp.

    Milligan, Carol J; Möller, Clemens


    Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as controlling many metabolic functions. Furthermore, ion channels are known to be involved in many disease states and as such have become popular therapeutic targets. For many years now manual patch-clamping has been regarded as one of the best approaches for assaying ion channel function, through direct measurement of ion flow across these membrane proteins. Over the last decade there have been many remarkable breakthroughs in the development of technologies enabling the study of ion channels. One of these breakthroughs is the development of automated planar patch-clamp technology. Automated platforms have demonstrated the ability to generate high-quality data with high throughput capabilities, at great efficiency and reliability. Additional features such as simultaneous intracellular and extracellular perfusion of the cell membrane, current clamp operation, fast compound application, an increasing rate of parallelization, and more recently temperature control have been introduced. Furthermore, in addition to the well-established studies of over-expressed ion channel proteins in cell lines, new generations of planar patch-clamp systems have enabled successful studies of native and primary mammalian cells. This technology is becoming increasingly popular and extensively used both within areas of drug discovery as well as academic research. Many platforms have been developed including NPC-16 Patchliner(®) and SyncroPatch(®) 96 (Nanion Technologies GmbH, Munich), CytoPatch™ (Cytocentrics AG, Rostock), PatchXpress(®) 7000A, IonWorks(®) Quattro and IonWorks Barracuda™, (Molecular Devices, LLC); Dynaflow(®) HT (Cellectricon

  19. Multifrequency Excitation of a Clamped-Clamped Microbeam

    Jaber, Nizar R.


    We present analytical and experimental investigation of an electrically actuated clamped-clamped microbeam under a twosource harmonic excitation. The first frequency is swept around the first mode of vibration where the second one is fixed. These microbeams are fabricated using polyimide as structural layer coated with nickel form top and chromium and gold layers from bottom. We demonstrate the excitation of additive and subtractive type resonance. We show that by properly tuning the frequency and the amplitude of the excitation force, the frequency bandwidth of the resonator is increased. Theoretically, we solved the eigenvalue problem for different axial forces to find the natural frequencies ratio that match the experimental values. Using Galerkin method, a reduced order model is derived to simulate the static and dynamic response of the device where using three symmetric mode shapes provided a good agreement with experimental data. © Copyright 2015 by ASME.

  20. Analysis list: Clamp [Chip-atlas[Archive

    Full Text Available Clamp Cell line + dm3 ...

  1. Oxygen clamps in gold nanowires

    Novaes, Frederico D.; da Silva, Antonio J. R.; da Silva, E. Z.; Fazzio, A.


    We investigate how the insertion of an oxygen atom in an atomically thin gold nanowire can affect its rupture. We find, using ab initio total energy density functional theory calculations, that O atoms when inserted in gold nanowires form not only stable but also very strong bonds, in such a way that they can extract atoms from a stable tip, serving in this way as a clamp that could be used to pull a string of gold atoms.

  2. Downhole Vibration Monitoring and Control System

    Martin E. Cobern


    The objective of this program is to develop a system to both monitor the vibration of a bottomhole assembly, and to adjust the properties of an active damper in response to these measured vibrations. The key feature of this system is its use of a magnetorheological fluid (MRF) to allow the damping coefficient to be changed extensively, rapidly and reversibly without the use of mechanical valves, but only by the application of a current. Phase I of this program, which entailed modeling and design of the necessary subsystems and design, manufacture and test of a full laboratory prototype, was completed on May 31, 2004. Much of the effort was devoted to the design and testing of the MRF damper, itself. The principal objectives of Phase II were: more extensive laboratory testing, including the evaluation of different feedback algorithms for control of the damper; design and manufacture of a field prototype system; and, testing of the field prototype in a drilling laboratory. Phase II concluded on January 31, 2006, and a final report was issued. Work on Phase III of the project began during the first quarter, 2006, with the objectives of building precommercial prototypes, testing them in a drilling laboratory and the field; developing and implementing a commercialization plan. All of these have been accomplished. The Downhole Vibration Monitoring & Control System (DVMCS) prototypes have been successfully proven in testing at the TerraTek drilling facility and at the Rocky Mountain Oilfield Test Center (RMOTC.) Based on the results of these tests, we have signed a definitive development and distribution agreement with Smith, and commercial deployment is underway. This current version of the DVMCS monitors and controls axial vibrations. Due to time and budget constraints of this program, it was not possible to complete a system that would also deal with lateral and torsional (stick-slip) vibrations as originally planned; however, this effort is continuing without DOE

  3. Radio Polarimetry of 3C119, 3C318, and 3C343 at milliarcsecond resolution

    Mantovani, F; Junor, W; Saikia, D J; Salter, C J


    We report VLBA polarimetric observations of the CSS sources 3C119, 3C318, and 3C343 at 5 and 8.4 GHz. The CSS source 3C119 has source rest-frame RM values up to ~10200 rad/m**2 in a region which coincides with a change in the direction of the inner jet. This component is located ~325 pc from the core, which is variable and has a peaked radio spectrum. In the case of 3C318, a rest-frame RM of ~3030 rad/m**2 has been estimated for the brightest component which contributes almost all of the polarised emission. Further, two more extended components have been detected, clearly showing "wiggles" in the jet towards the southern side of the source. The CSS source 3C343 contains two peaks of emission and a curved jet embedded in more diffuse emission. It exhibits complex field directions near the emission peaks, which indicate rest-frame RM values in excess of ~6000 rad/m**2. The locations of the cores in 3C318 and 3C343 are not clear. The available data on mas-scale rest-frame RM estimates for CSS sources show that t...

  4. Loading dynamics of a sliding DNA clamp.

    Cho, Won-Ki


    Sliding DNA clamps are loaded at a ss/dsDNA junction by a clamp loader that depends on ATP binding for clamp opening. Sequential ATP hydrolysis results in closure of the clamp so that it completely encircles and diffuses on dsDNA. We followed events during loading of an E. coli β clamp in real time by using single-molecule FRET (smFRET). Three successive FRET states were retained for 0.3 s, 0.7 s, and 9 min: Hydrolysis of the first ATP molecule by the γ clamp loader resulted in closure of the clamp in 0.3 s, and after 0.7 s in the closed conformation, the clamp was released to diffuse on the dsDNA for at least 9 min. An additional single-molecule polarization study revealed that the interfacial domain of the clamp rotated in plane by approximately 8° during clamp closure. The single-molecule polarization and FRET studies thus revealed the real-time dynamics of the ATP-hydrolysis-dependent 3D conformational change of the β clamp during loading at a ss/dsDNA junction.

  5. The RFC clamp loader: structure and function.

    Yao, Nina Y; O'Donnell, Mike


    The eukaryotic RFC clamp loader couples the energy of ATP hydrolysis to open and close the circular PCNA sliding clamp onto primed sites for use by DNA polymerases and repair factors. Structural studies reveal clamp loaders to be heteropentamers. Each subunit contains a region of homology to AAA+ proteins that defines two domains. The AAA+ domains form a right-handed spiral upon binding ATP. This spiral arrangement generates a DNA binding site within the center of RFC. DNA enters the central chamber through a gap between the AAA+ domains of two subunits. Specificity for a primed template junction is achieved by a third domain that blocks DNA, forcing it to bend sharply. Thus only DNA with a flexible joint can bind the central chamber. DNA entry also requires a slot in the PCNA clamp, which is opened upon binding the AAA+ domains of the clamp loader. ATP hydrolysis enables clamp closing and ejection of RFC, completing the clamp loading reaction.


    Huan-RueiShiu; Feng-ChuOu; Sih-LiChen


    A new 3C duct design method is proposed for designing a high quality, energy-efficiency cost-effective air duct system. It not only considers the demand of volume flow rate, but also takes into consideration a number of issues including system pressure balance, noise, vibration, space limitation and total system cost. This new method comprises three major calculation procedures:initial computer-aided design (CAD), computer-aided simulation (CAS) and correction processes (CP). An example is presented in this study to understand the characteristics of 3C method. It shows that 3C duct design method provides a simple computation procedure for an optimum air duct system. It also shortens the design schedule, prevents human calculation errors, and reduces the dependence on designer experience. In addition to apply in a new duct system design, 3C duct design method is also a powerful design tool for the expansion of an existing duct system.

  7. How Does Patch Clamp Work?--An easy approach to understanding patch clamp mechanism

    关兵才; 李之望


    In order to help patch clamp users,most of whom are not good enough at electrics and electronics,to understandthe complicated principles of patch clamp technique,we present a readily accessible explanation of patch clamp mechanism,in-cluding basic analysis of membrane currents under voltage clamp,achievement of voltage clamp and current recording,capaci-tance compensation,series resistance compensation,voltage offset correction,leak subtraction and current clamp,based on ourworking and teaching experience and with the aid of some useful formulae.

  8. Distributed Temperature Sensing as a downhole tool in hydrogeology

    Bense, V.F.; Read, T.; Bour, O.; Borgne, Le T.; Coleman, T.; Krause, S.; Chalari, A.; Mondanos, M.; Ciocca, F.; Selker, J.S.


    Distributed Temperature Sensing (DTS) technology enables downhole temperature monitoring to study hydrogeological processes at unprecedentedly high frequency and spatial resolution. DTS has been widely applied in passive mode in site investigations of groundwater flow, in-well flow, and subsurfac

  9. Development of a novel vortex flow meter for downhole use

    Schiferli, W.; Cheng, L.K.


    Due to the increasing complexity of oil and gas wells, the demand for instrumentation to measure conditions inside well tubing below the surface is growing rapidly. A robust meter was designed to measure liquid flows at downhole conditions. The meter is based on a specially-designed bluff body to de

  10. Research on Overflow Monitoring Mechanism Based on Downhole Microflow Detection

    Liang Ge


    Full Text Available The flow rate variation of the drilling fluid and micro-overflow loss is difficult to analyze. The purpose to prevent the occurrence of kick, lost circulation, and other complex conditions is not easy to be achieved. Therefore, the microflow-induced annulus multiphase flow rate and annulus pressure field model were studied, and a downhole microflow measurement system has been developed. A differential pressure type flow measurement was used in the system, and real-time downhole information was obtained to achieve deep, narrow windows and other safety-density complex formation security. This paper introduced a new bottom-hole flow meter which can measure the annular flux while drilling and monitor overflow and circulation loss. The accuracy and reliability of the MPD (managed pressure drilling system can be improved obviously by applying the device; as a result, the safety of drilling is enhanced and the cost is reduced.

  11. High power laser downhole cutting tools and systems

    Zediker, Mark S; Rinzler, Charles C; Faircloth, Brian O; Koblick, Yeshaya; Moxley, Joel F


    Downhole cutting systems, devices and methods for utilizing 10 kW or more laser energy transmitted deep into the earth with the suppression of associated nonlinear phenomena. Systems and devices for the laser cutting operations within a borehole in the earth. These systems and devices can deliver high power laser energy down a deep borehole, while maintaining the high power to perform cutting operations in such boreholes deep within the earth.

  12. Program to develop improved downhole drilling motors. Semiannual report

    Maurer, W.C.


    The following are described: the history of turbodrill development, positive displacement motor development, the theory of turbodrills, the theory of positive displacement motors, basic motor components, forces on thrust bearings, thrust bearing design, radial bearing design, rotary seal design, sealed lubrication system, lubricants, and project status. Included in appendices are materials on high-temperature lubricants and a progress report on the design of downhole motor seal, bearing, and lubrication test systems. (MHR)

  13. Mechanical Analysis of the Down-Hole String in a Deep Well

    HE Yu-fa; LIU Qing-you; ZHU Xiao-lan


    In a deep well, under high pressure and temperature, the force, deformation, and movement of the downhole string are very complicated. In this paper, based on the analysis of the working environment and load properties of the down-hole string in deep wells, the factors of high temperature, high pressure, and the complex structure of wells have been taken into consideration. The loads of the down-hole string, stability, back buckle configuration, dynamic characteristics, mechanical strength, and deformation have been systematically studied. On this basis, mechanical models of a down-hole string under different conditions have been established and software for aiding analysis has been developed. By using this software, deep well strings under different construction parameters can be simulated and analyzed, providing a theoretical basis and method for down-hole string assembly, as well as string down-hole construction and operation.

  14. Dynamic clamp with StdpC software.

    Kemenes, Ildikó; Marra, Vincenzo; Crossley, Michael; Samu, Dávid; Staras, Kevin; Kemenes, György; Nowotny, Thomas


    Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based on a fast cycle of measuring the membrane potential of a cell, calculating the current of a desired simulated component using an appropriate model and injecting this current into the cell. Here we present a dynamic clamp protocol using free, fully integrated, open-source software (StdpC, for spike timing-dependent plasticity clamp). Use of this protocol does not require specialist hardware, costly commercial software, experience in real-time operating systems or a strong programming background. The software enables the configuration and operation of a wide range of complex and fully automated dynamic clamp experiments through an intuitive and powerful interface with a minimal initial lead time of a few hours. After initial configuration, experimental results can be generated within minutes of establishing cell recording.

  15. Clamp force and alignment checking device

    Spicer, John Patrick; Cai, Wayne W.; Chakraborty, Debejyo; Mink, Keith


    A check fixture measures a total clamp force applied by a welder device. The welder device includes a welding horn having a plurality of weld pads and welding anvil having a plurality of weld pads. The check fixture includes a base member operatively supporting a plurality of force sensors. The base member and the force sensors are received between the weld pads of the welding horn and the anvil pads of the welding anvil. Each force sensor is configured to measure an individual clamp force applied thereto by corresponding weld and anvil pads when the base member is received between the welding horn and the welding anvil and the welder device is in the clamped position. The individual clamp forces are used to determine whether the weld and/or anvil pads are worn or misaligned.

  16. Molecular Mechanisms of DNA Polymerase Clamp Loaders

    Kelch, Brian; Makino, Debora; Simonetta, Kyle; O'Donnell, Mike; Kuriyan, John

    Clamp loaders are ATP-driven multiprotein machines that couple ATP hydrolysis to the opening and closing of a circular protein ring around DNA. This ring-shaped clamp slides along DNA, and interacts with numerous proteins involved in DNA replication, DNA repair and cell cycle control. Recently determined structures of clamp loader complexes from prokaryotic and eukaryotic DNA polymerases have revealed exciting new details of how these complex AAA+ machines perform this essential clamp loading function. This review serves as background to John Kuriyan's lecture at the 2010 Erice School, and is not meant as a comprehensive review of the contributions of the many scientists who have advanced this field. These lecture notes are derived from recent reviews and research papers from our groups.

  17. Ergonomic redesign and evaluation of a clamping tool handle.

    Jung, Myung-Chul; Hallbeck, M Susan


    The handle of a commercial bar clamp was redesigned using ergonomic principles and then compared with an original clamp. Ten male and ten female students participated in simulated clamping tasks under various conditions, including different clamping heights, clamping methods, and handle-gripping methods, with respect to the dependent variables of clamping and handle-squeezing forces. The results showed that the redesigned clamp produced larger clamping force with lower handle-squeezing forces than the original clamp. As expected, males exerted more force than females in both clamping and squeezing forces. A pistol grip method was superior to an upside-down handle-gripping method. Two-handed operation was recommended for this type of clamp by simultaneously pulling the trigger and sliding the bar in order to initially tighten around objects. This study shows that the application of ergonomic guidelines increases the efficiency and usability of manual handtools.

  18. Downhole tools can increase deep drilling rig productivity

    Thiessen, D.A.; Doiron, H.H.


    Explains how there is much to be gained by better utilization of current drilling equipment, particularly for the much more expensive deep drilling. Optimized mud weight, better hydraulics, and higher rpm offer the best opportunities with increased bit weight as a fourth possibility. Current RandD in such areas as shock absorbers, downhole motors, new materials and designs for bits, and improved instrumentation will decrease drilling costs. Concludes that a reasonable projection is that drilling time for deep wells can be halved within the next decade.

  19. Piezoresistive cantilever force-clamp system

    Park, Sung-Jin; Petzold, Bryan C.; Pruitt, Beth L. [Department of Mechanical Engineering, Stanford University, Stanford, California 94305 (United States); Goodman, Miriam B. [Department of Molecular and Cellular Physiology, Stanford University, Stanford, California 94305 (United States)


    We present a microelectromechanical device-based tool, namely, a force-clamp system that sets or ''clamps'' the scaled force and can apply designed loading profiles (e.g., constant, sinusoidal) of a desired magnitude. The system implements a piezoresistive cantilever as a force sensor and the built-in capacitive sensor of a piezoelectric actuator as a displacement sensor, such that sample indentation depth can be directly calculated from the force and displacement signals. A programmable real-time controller operating at 100 kHz feedback calculates the driving voltage of the actuator. The system has two distinct modes: a force-clamp mode that controls the force applied to a sample and a displacement-clamp mode that controls the moving distance of the actuator. We demonstrate that the system has a large dynamic range (sub-nN up to tens of {mu}N force and nm up to tens of {mu}m displacement) in both air and water, and excellent dynamic response (fast response time, <2 ms and large bandwidth, 1 Hz up to 1 kHz). In addition, the system has been specifically designed to be integrated with other instruments such as a microscope with patch-clamp electronics. We demonstrate the capabilities of the system by using it to calibrate the stiffness and sensitivity of an electrostatic actuator and to measure the mechanics of a living, freely moving Caenorhabditis elegans nematode.

  20. Arterial clamping leads to stenosis at clamp sites after femoropopliteal bypass surgery

    Vriens, Bianca H. R.; Pol, Robert A.; Hulsebos, Robin G.; van Det, Rob J.; van der Palen, Job; Zeebregts, Clark J.; Geelkerken, Robert H.


    BACKGROUND: To date, the incidence and clinical relevance of arterial stenosis at clamp sites after femoropopliteal bypass surgery is unknown. METHODS: Ninety-four patients underwent a femoropopliteal bypass in which the arterial inflow and outflow clamp sites were controlled by the Fogarty-Soft-Inl

  1. Arterial clamping leads to stenosis at clamp sites after femoropopliteal bypass surgery

    Vriens, Bianca H.R.; Pol, Robert A.; Hulsebos, Robin G.; Det, van Rob J.; Palen, van der Job; Zeebregts, Clark J.; Geelkerken, Robert H.


    Background To date, the incidence and clinical relevance of arterial stenosis at clamp sites after femoropopliteal bypass surgery is unknown. Methods Ninety-four patients underwent a femoropopliteal bypass in which the arterial inflow and outflow clamp sites were controlled by the Fogarty-Soft-Inla

  2. Vascular clamping in liver surgery: physiology, indications and techniques

    Cherqui Daniel


    Full Text Available Abstract This article reviews the historical evolution of hepatic vascular clamping and their indications. The anatomic basis for partial and complete vascular clamping will be discussed, as will the rationales of continuous and intermittent vascular clamping. Specific techniques discussed and described include inflow clamping (Pringle maneuver, extra-hepatic selective clamping and intraglissonian clamping and outflow clamping (total vascular exclusion, hepatic vascular exclusion with preservation of caval flow. The fundamental role of a low Central Venous Pressure during open and laparoscopic hepatectomy is described, as is the difference in their intra-operative measurements. The biological basis for ischemic preconditioning will be elucidated. Although the potential dangers of vascular clamping and the development of modern coagulation devices question the need for systemic clamping; the pre-operative factors and unforseen intra-operative events that mandate the use of hepatic vascular clamping will be highlighted.

  3. IMPORTANT: Fluke is recalling Digital Clamp Meters


    Fluke is voluntarily recalling four models of Digital Clamp Meters: Fluke 373, 374, 375 and 376. If you own one of these clamp meters, please stop using it and send it back to Fluke for repair even if you have not experienced problems.   Description of the problem: "The printed circuit assembly may not be properly fastened to the test lead input jack. This may result in inaccurate voltage readings, including a low or no-voltage reading on a circuit energised with a hazardous voltage, presenting a shock, electrocution or thermal burn hazard." To determine if your clamp meter is affected by this recall notice, and for more information, click here.

  4. Design and application of multifunctional stomach clamp

    Yun Fu Lu; Pi Wu Li; Xir Xin Zhang


    AIM Pylorus and pyloric vagus preserving gastrectomy (PPVPG) is an efficient operation mode for treatinggastric and duodenal ulcers. For its better application and popularization, we have designed andmanufactured a special multifunctional stomach clamp.METHODS The clamp has been designed carefully in view of the different sizes of the patients' stomachsand the different starting points of Latarjer nerves so as to facilitate the execution of the present operationmode, which requires the preservation of the vagus innervated pyloric region so as to make the remnantstomach maintain its normal physiological function and the vagus branches clinging antral seromuscular flapkept in proper size so that avoiding too much remaining antral mucosa is removed to cause greater difficultyin operation. With this in view, we observed and measured the distance between the Latarjer nerves distribution and the pyloric rings of 42 patients' stomachs in the course of operations. We bent an aluminumstring 3 mm thick for each stomach according to the size of antral seromuecular flap and chose an optimalcurvature from the 42 sizes for manufacturing the clamp. The clamp is made of 3 Cr13 stainless steel,consisting of three parts, the curved head, the fastening ring and the handle. The curved head is shapedalmost exactly like the remained antral seromuscular flap. The ring at its tip when in place fastens the twosides of the clamp so that the tissue of the stomach between will be clutched by an even force. On one sideare three cuts for moving the ring upwards, each cut representing 1 mm for fastening and adjustments can bemade in view of the thickness or thinness of the tissue. The handles bend backward for fighting shy of thecuts so as to facilitate the operation.RESULTS With the stomach disassociated in the light of PPVPG procedures, the clamp may easily beapplied from the upper part of the Latarjer nerves. The following merits can be noted. ① The curving line ofthe flap can be precisely

  5. Compact, Stiff, Remotely-Actuable Quick-Release Clamp

    Tsai, Ted W. (Inventor)


    The present invention provides a clamp that is compact and lightweight, yet provides high holding strength and stiffness or rigidity. The clamp uses a unique double slant interface design which provides mechanical advantages to resist forces applied to the clamp member as the load increases. The clamp allows for rapid and remote-activated release of the clamp jaws by applying only a small operating force to an over-center lock/release mechanism, such as by pulling a manual tether.

  6. Downhole stuffing box aids pumping of heavy crude

    Franco, A.


    Petroleos Mexicanos (Pemex) has found a new way to produce heavy oil from pumping wells. The key component is a bottomhole stuffing box which reduces the friction of the pumping string during the downstroke. The relatively simple system, already proven successful in the Ebano-Panuco area of Mexico, could be easily applied wherever operators are faced with the task of producing highly viscous oil and coping with the problem of constant sucker-rod string breakage. Basically, Pemex' method consists of a special downhole stuffing box and polished rod anchored at the end of the pumping string. The isolated tubing is filled with water to minimize friction, and the heavy oil is produced through the annular space between the tubing and casing strings. With Pemex' new downhole stuffing box, friction is minimized. Even though the system is applicable to practically any type of completion (any size of tubing and casing), Pemex has found it to be ideally suited for wells having 8-5/8-8in. casing or larger. The accompanying line diagram of a hookup for a well with 8-5/8-in. casing and 3-in. tubing shows the necessary installations. The stuffing box features a groove bolt at the top to allow tightening of a packing element without having to remove the assembly from the hole.

  7. Downhole control of deviation with steerable straight-hole turbodrills

    Gaynor, T.M.


    Advances in directional drilling have until recently been confined to issues that are peripheral to the central problem of controlling assembly behavior downhole. Examples of these advances are measurement while drilling (MWD) and the increasing use of computer assistance in well planning. These were significant steps forward, but the major problem remained. Changes in formation deviation tendencies led to trips to change bottomhole assemblies (BHA's) to cope with the new conditions. There is almost no direct control of deviation behavior. The steerable straight-hole turbodrill (SST) addresses this problem directly, allowing alteration of the well course without the need to trip. The availability of such a system radically changes the way in which directional well planning may be approached. This paper describes the equipment used and the equipment's construction and operational requirements. It discusses the capabilities and current limitation of the systems. Field results are presented for some 300,000 ft (91 500 m) of deviated drilling carried out over 2 years in Alaska and the North Sea. A series of four highly deviated wells totaling 35,000 ft (10 700m) with only three deviation trips is included. The SST is the first deviation drilling system to achieve deviation control over long sections without tripping to change BHA's. Bits and downhole equipment are now more reliable and long-lived than ever, therefore, deviation trips are becoming a major target for well cost saving.

  8. Prolonged penile strangulation with metal clamps

    Chirag Patel; Richard Kim; Michael Delterzo; Run Wang


    Various different objects have been reported to strangulate the penis. We reported on a patient who used metal radiator clamps for an extended period of time. Workup included history, physical examination and urinalysis. The patient was taken to the operating room for further evaluation with cystourethroscopy and orthopedic wire cutters were used to break the metal bands.

  9. Cerebral haemodynamics during carotid cross-clamping.

    Pistolese, G R; Ippoliti, A; Appolloni, A; Ronchey, S; Faraglia, V


    Carotid artery cross-clamping ischaemia during carotid endarterectomy (CEA) causes 5-30% of perioperative neurological deficits. This study was performed to identify possible clinical situations at higher risk for carotid cross-clamping ischaemia. 606 consecutive patients underwent CEA and were retrospectively studied; they were grouped according to risk factors, presence of associated vascular diseases, clinical pattern, angiographic and CT scan findings. Stump pressure measurement was provided in all patients, perioperative monitoring during CEA was performed by electroencephalogram (EEG) in 469 (77%) and somatosensorial evoked potentials (SEP) in 137 (23%). Local anaesthesia was used in 88 (14.5%) patients. Ischaemic changes during carotid cross-clamping were registered in 118 patients (19.5%). The incidence of cross-clamping ischaemia was then related to different factors; it affected 5.6% of asymptomatics, 25.4% of patients with fixed stroke and 38.5% of those with stenosis and contralateral occlusion. Angiographic and clinical correlation showed that patients with more severe lesions are mostly affected by clamping ischaemia (up to 55% in those with stroke and stenosis with contralateral occlusion). Age, hypertension and diabetes do not significantly affect incidence of ischaemic changes. Positive CT scan increased this risk; statistical relevance was found in regard to patients with unilateral or bilateral stenosis and in those with transient ischaemic attacks. A higher risk can be expected for subjects with more severe clinical and instrumental findings, even if no patients can be considered completely at risk or risk free. Perioperative monitoring is always mandatory and is of great importance in detecting ischaemic changes and preventing cerebral damage using a temporary intraluminal shunt.

  10. Comparative evaluation of surface and downhole steam-generation techniques

    Hart, C.

    The application of heat to reservoirs containing high API gravity oils can substantially improve recovery. Although steam injection is currently the principal thermal recovery method, heat transmission losses associated with delivery of the steam from the surface generators to the oil bearing formation has limited conventional steam injection to shallow reservoirs. The objective of the Department of Energy's Project DEEP STEAM is to develop the technology required to economically produce heavy oil from deep reservoirs. The tasks included in this effort are the development and evaluation of thermally efficient delivery systems and downhole steam generation systems. The technical and economic performance of conventional surface steam drives, which are strongly influenced by heat losses are compared. The selection of a preferred technology based upon either total efficiency or cost is found to be strongly influenced by reservoir depth, steam mass flow rate, and sandface steam quality.

  11. 275 C Downhole Switched-Mode Power Supply

    Chris Hutchens; Vijay Madhuravasal


    A vee-square (V2) control based controller IC is developed for a switch mode power supply capable of operating at extreme temperature/harsh environment conditions. A buck type regulator with silicon carbide power junction field effect transistors (JFET) as power devices is used to analyze the performance of controller. Special emphases are made on the analog sub-blocks--voltage reference, operational transconductance amplifier and comparator as individual building blocks. Transformer coupled gate drives and high temperature operable magnetic cores and capacitors are identified and tested for use in the design. Conventional ceramic chip packaging of ICs combined with lead carrier type mounting of passive filter components is introduced for hybrid packaging of the complete product. The developed SMPS is anticipated to support the operation of down-hole microcontrollers and other electronics devices that require low/medium power filtered dc inputs over an operating temperature of 275 C.

  12. Experimental studies of operating characteristics of a downhole electric heater

    Simkin, E.M.; Sergeev, A.I.; Sheinman, A.B.


    A new type of electric downhole heater for deparaffinization and heating of formations in oil wells is described. The heater is a hollow hermetic cylinder, which contains an electrode insulated from the body of the heater. The cylinder contains a predetemined volume of water of fixed electrical resistance. Resistance of this water is adjusted by addition of salt. A cable supplying the electrode with electric current also ground the heater by body. The current passes through the water, heats it, and produces steam. The steam heats up the hermetically sealed body of the heater, and heat flows to the well fluid around the heater. A schematic diagram of the heater is shown. Thermal output of the heater depends on current supplied, dimension of heater and components, resistance and volume of water in heater, and other parameters. All these factors are correlated with heat output by a series of graphs and equations.

  13. Rotating pulse valve for downhole fluid telemetry systems

    Duckworth, A.


    An apparatus for generating pressure pulses in a drilling fluid in a drill collar section of a drill string is described comprising: rotating valve means substantially diametrically mounted in a drill string segment, said rotating valve means alternating between a first position corresponding to more resistance to the flow of drilling fluid and a second position corresponding to less resistance to the flow of drilling fluid, said rotating valve means being impelled by the flow of drilling fluid; and restraining means disposed in the drill collar segment, said restraining means restraining said rotating valve means in said first position and releasing said rotating valve means from said first position in response to control signals indicative of a downhole condition.

  14. Dynamics and Stability of Pinned-Clamped and Clamped-Pinned Cylindrical Shells Conveying Fluid

    Misra, A. K.; Wong, S. S. T.; Païdoussis, M. P.


    The paper examines the dynamics and stability of fluid-conveying cylindrical shells having pinned-clamped or clamped-pinned boundary conditions, where ``pinned'' is an abbreviation for ``simply supported''. Flügge's equations are used to describe the shell motion, while the fluid-dynamic perturbation pressure is obtained utilizing the linearized potential flow theory. The solution is obtained using two methods - the travelling wave method and the Fourier-transform approach. The results obtained by both methods suggest that the negative damping of the clamped-pinned systems and positive damping of the pinned-clamped systems, observed by previous investigators for any arbitrarily small flow velocity, are simply numerical artefacts; this is reinforced by energy considerations, in which the work done by the fluid on the shell is shown to be zero. Hence, it is concluded that both systems are conservative.

  15. Lithostratigraphy from downhole logs in Hole AND-1B, Antarctica

    Williams, Trevor; Morin, Roger H.; Jarrard, Richard D.; Jackolski, Chris L.; Henrys, Stuart A.; Niessen, Frank; Magens, Diana; Kuhn, Gerhard; Monien, Donata; Powell, Ross D.


    The ANDRILL (Antarctic Drilling Project) McMurdo Ice Shelf (MIS) project drilled 1285 m of sediment in Hole AND–1B, representing the past 12 m.y. of glacial history. Downhole geophysical logs were acquired to a depth of 1018 mbsf (meters below seafloor), and are complementary to data acquired from the core. The natural gamma radiation (NGR) and magnetic susceptibility logs are particularly useful for understanding lithological and paleoenvironmental change at ANDRILL McMurdo Ice Shelf Hole AND–1B. NGR logs cover the entire interval from the seafloor to 1018 mbsf, and magnetic susceptibility and other logs covered the open hole intervals between 692 and 1018 and 237–342 mbsf. In the upper part of AND–1B, clear alternations between low and high NGR values distinguish between diatomite (lacking minerals containing naturally radioactive K, U, and Th) and diamictite (containing K-bearing clays, K-feldspar, mica, and heavy minerals). In the lower open hole logged section, NGR and magnetic susceptibility can also distinguish claystones (rich in K-bearing clay minerals, relatively low in magnetite) and diamictites (relatively high in magnetite). Sandstones can be distinguished by their high resistivity values in AND–1B. On the basis of these three downhole logs, diamictite, claystones, and sandstones can be predicted correctly for 74% of the 692–1018 mbsf interval. The logs were then used to predict facies for the 6% of this interval that was unrecovered by coring. Given the understanding of the physical property characteristics of different facies, it is also possible to identify subtle changes in lithology from the physical properties and help refine parts of the lithostratigraphy, for example, the varying terrigenous content of diatomites and the transitions from subice diamictite to open-water diatomite.

  16. Mechanical and metallurgical properties of carotid artery clamps.

    Dujovny, M; Kossovsky, N; Kossowsky, R; Segal, R; Diaz, F G; Kaufman, H; Perlin, A; Cook, E E


    The mechanical and metallurgical properties of carotid artery clamps were evaluated. The pressure plate retreat propensity, metallurgical composition, surface morphology, magnetic properties, and corrosion resistance of the Crutchfield, Selverstone, Salibi, and Kindt clamps were tested. None of the clamps showed evidence of pressure plate retreat. The clamps differed significantly in their composition, surface cleanliness, magnetic properties, and corrosion resistance. The Crutchfield clamp was the only one manufactured from an ASTM-ANSI-approved implantable stainless steel (AISI 316) and the only clamp in which the surfaces were clean and free of debris. The Selverstone clamp was made principally from AISI 304 stainless steel, as was one Salibi clamp. The pressure plate on another Salibi clamp was made from a 1% chromium and 1% manganese steel. Machining and surface debris consisting principally of aluminum, silicon, and sulfur was abundant on the Selverstone and Salibi clamps. The Kindt clamp was manufactured from AISI 301 stainless steel with a silicate-aluminized outer coating. The Crutchfield and Selverstone clamps were essentially nonferromagnetic, whereas the Salibi and Kindt clamps were sensitive to magnetic flux. In the pitting potential corrosion test, the Crutchfield clamp demonstrated good corrosion resistance with a pitting potential of 310 mV and no surface corrosion or pitting by scanning electron microscopy examination. The Selverstone clamp had lower pitting potentials and showed various degrees of corrosion and surface pitting by scanning electron microscopy. The Salibi pressure plate had a very low pitting potential of -525 mV and showed severe corrosion. By metallurgical criteria, only the Crutchfield clamp is suitable for long term implantation.



    An active valve for use e.g. in fluidic microsystems is provided, wherein the active valve comprises a membrane having at least one flow gate, arranged between a first and a second substantially rigid element. Adjusting means provides an adjustment of the clamping force on membrane arranged between...... the first and second substantially rigid element. Hereby the flow rate through the active valve can be continuously and precisely controlled....

  18. Γ-source Neutral Point Clamped Inverter

    Mo, Wei; Loh, Poh Chiang; Blaabjerg, Frede

    Transformer based Z-source inverters are recently proposed to achieve promising buck-boost capability. They have improved higher buck-boost capability, smaller size and less components count over Z-source inverters. On the other hand, neutral point clamped inverters have less switching stress...... and better output performance comparing with traditional two-level inverters. Integrating these two types of configurations can help neutral point inverters achieve enhanced votlage buck-boost capability....

  19. Vibration of an eccentrically clamped annular plate

    Tseng, J.-G.; Wickert, J. A.


    Small amplitude vibration of an eccentric annular plate, which is free along its outer edge and clamped along the interior, is investigated through experimental and analytical methods. A disk with this geometry, or a stacked array in which the clamping and symmetry axes of each disk are nominally coincident, is common in data storage and brake systems applications. In the present case, the geometric imperfections on the boundary can have important implications for the disk's dynamic response. Changes that occur in the natural frequency spectrum, the mode shapes, and the free response under eccentric mounting are studied through laboratory measurements and an approximate discrete model of the plate. The natural frequencies and modes are found through global discretization of the Kamke quotient for a classical thin plate. For the axisymmetric geometry, the natural frequencies of the sine and cosine vibration modes for a specified number of nodal diameters are repeated. With increasing eccentricity, on the other hand, each pair of repeated frequencies splits at a rate that depends on the number of nodal diameters. Over a range of clamping and eccentricity ratios, the model's predictions are compared to the measured results.

  20. Single molecule study of a processivity clamp sliding on DNA

    Laurence, T A; Kwon, Y; Johnson, A; Hollars, C; O?Donnell, M; Camarero, J A; Barsky, D


    Using solution based single molecule spectroscopy, we study the motion of the polIII {beta}-subunit DNA sliding clamp ('{beta}-clamp') on DNA. Present in all cellular (and some viral) forms of life, DNA sliding clamps attach to polymerases and allow rapid, processive replication of DNA. In the absence of other proteins, the DNA sliding clamps are thought to 'freely slide' along the DNA; however, the abundance of positively charged residues along the inner surface may create favorable electrostatic contact with the highly negatively charged DNA. We have performed single-molecule measurements on a fluorescently labeled {beta}-clamp loaded onto freely diffusing plasmids annealed with fluorescently labeled primers of up to 90 bases. We find that the diffusion constant for 1D diffusion of the {beta}-clamp on DNA satisfies D {le} 10{sup -14} cm{sup 2}/s, much slower than the frictionless limit of D = 10{sup -10} cm{sup 2}/s. We find that the {beta} clamp remains at the 3-foot end in the presence of E. coli single-stranded binding protein (SSB), which would allow for a sliding clamp to wait for binding of the DNA polymerase. Replacement of SSB with Human RP-A eliminates this interaction; free movement of sliding clamp and poor binding of clamp loader to the junction allows sliding clamp to accumulate on DNA. This result implies that the clamp not only acts as a tether, but also a placeholder.

  1. 21 CFR 882.4460 - Neurosurgical head holder (skull clamp).


    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Neurosurgical head holder (skull clamp). 882.4460 Section 882.4460 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... holder (skull clamp). (a) Identification. A neurosurgical head holder (skull clamp) is a device used...

  2. Multilevel-clamped multilevel converters (MLC2)

    Rodriguez, Pedro; Bellar, Maria Dias; Muñoz-Aguliar, Raúl Santiago;


    This letter introduces a new series of multilevel (ML) converters based on the ML clamping concept. By applying this technique, a ML clamping unit (MCU) conveys additional levels for synthesizing the output waveforms of a diode-clampedML dc– ac power converter. The basic building block of the ML ...

  3. Downhole water flow controller for aquifer storage recovery wells

    Pyne, R.D.


    This patent describes a downhole flow control device for continuous automatic control of water flowing into or out of wells, aquifers and the like through pipe columns. The upper end of the first tubular member is mounted to the pipe column so as to be in fluid communication therewith. The lower end of the first tubular member is substantially closed. A second tubular member is mounted concentrically within and proximate to the first tubular member and has an open upper end and side walls and a substantially closed lower end. First openings are spaced in vertical relationship to the second openings. Third openings are through the second tubular member. The second tubular member is vertically movable with respect to the first tubular member so as to selectively align the third openings with either of the first and second openings. Biasing means are located between the lower ends of the first and second tubular members for normally urging the second tubular member vertically upward with respect to the first tubular member. The biasing means are yieldable upon the introduction of water into the pump column to permit the second tubular member to be vertically displaced relative to the first tubular member to thereby close the third openings with respect to the first or second openings. The third openings align with one of the first and second openings dependent upon the direction of fluid flow within the pipe column.

  4. Optimization of a clamped plate silencer.

    Wang, Chunqi; Han, Jun; Huang, Lixi


    A previous theoretical study [L. Huang, J. Acoust. Soc. Am. 119, 2628-2638 (2006)] shows that, in a duct, a simply supported plate covering a side-branch rigid cavity can function effectively as a wave reflector over a broad range of low to medium frequencies. In this study, analytical formulation is extended to the boundary condition of clamped plate, which is easier to implement in practice. The theoretical model is tested experimentally using balsawood, which has a very high stiffness to mass ratio. The spectral peaks and shapes of the measured TL are in agreement with those calculated theoretically, attempts are also made to account for the considerable sound absorption in the rig. Further numerical studies based on the validated model show that, for a uniform plate, the optimal stopband is narrower and the lower band limit is worse than that of the simply supported configuration. However, a wave reflector using nonuniform, clamped plates with thinner ends out-performs the simply supported configuration in every aspect. Analyses show that the improvement is attributed to the increased acoustic radiation efficiency over the bulk length of the nonuniform plate, which behaves more like a rigid plate.

  5. NATO-3C/Delta launch


    NATO-3C, the third in a series of NATO defense-related communication satellites, is scheduled to be launched on a delta vehicle from the Eastern Test Range no earlier than November 15, 1978. NATO-3A and -3B were successfully launched by Delta vehicles in April 1976 and January 1977, respectively. The NATO-3C spacecraft will be capable of transmitting voice, data, facsimile, and telex messages among military ground stations. The launch vehicle for the NATO-3C mission will be the Delta 2914 configuration. The launch vehicle is to place the spacecraft in a synchronous transfer orbit. The spacecraft Apogee Kick motor is to be fired at fifth transfer orbit apogee to circularize its orbit at geosynchronous altitude of 35,900 km(22,260 miles) above the equator over the Atlantic Ocean somewhere between 45 and 50 degrees W longitude.

  6. A Modelling Study for Predicting Life of Downhole Tubes Considering Service Environmental Parameters and Stress

    Tianliang Zhao


    Full Text Available A modelling effort was made to try to predict the life of downhole tubes or casings, synthetically considering the effect of service influencing factors on corrosion rate. Based on the discussed corrosion mechanism and corrosion processes of downhole tubes, a mathematic model was established. For downhole tubes, the influencing factors are environmental parameters and stress, which vary with service duration. Stress and the environmental parameters including water content, partial pressure of H2S and CO2, pH value, total pressure and temperature, were considered to be time-dependent. Based on the model, life-span of an L80 downhole tube in oilfield Halfaya, an oilfield in Iraq, was predicted. The results show that life-span of the L80 downhole tube in Halfaya is 247 months (approximately 20 years under initial stress of 0.1 yield strength and 641 months (approximately 53 years under no initial stress, which indicates that an initial stress of 0.1 yield strength will reduce the life-span by more than half.

  7. Imaging hydraulic fractures by microseismic migration for downhole monitoring system

    Lin, Ye; Zhang, Haijiang


    It has been a challenge to accurately characterize fracture zones created by hydraulic fracturing from microseismic event locations. This is because generally detected events are not complete due to the associated low signal to noise ratio and some fracturing stages may not produce microseismic events even if fractures are well developed. As a result, spatial distribution of microseismic events may not well represent fractured zones by hydraulic fracturing. Here, we propose a new way to characterize the fractured zones by reverse time migration (RTM) of microseismic waveforms from some events. This is based on the fact that fractures filled with proppants and other fluids can act as strong scatterers for seismic waves. Therefore, for multi-stage hydraulic fracturing, recorded waveforms from microseismic events induced in a more recent stage may be scattered by fractured zones from previous stages. Through RTM of microseismic waveforms in the current stage, we can determine fractured zones created in previous stages by imaging area of strong scattering. We test the feasibility of this method using synthetic models with different configurations of microseismic event locations and borehole sensor positions for a 2D downhole microseismic monitoring system. Synthetic tests show that with a few events fractured zones can be directly imaged and thus the stimulated reservoir volume (SRV) can be estimated. Compared to the conventional location-based SRV estimation method, the proposed new method does not depend on the completeness of detected events and only a limited number of detected and located events are necessary for characterizing fracture distribution. For simplicity, the 2D model is used for illustrating the concept of microseismic RTM for imaging the fracture zone but the method can be adapted to real cases in the future.

  8. Analyzing polarization swings in 3C 279

    Kiehlmann, S; Jorstad, S G; Sokolovsky, K V; Schinzel, F K; Agudo, I; Arkharov, A A; Benitez, E; Berdyugin, A; Blinov, D A; Bochkarev, N G; Borman, G A; Burenkov, A N; Casadio, C; Doroshenko, V T; Efimova, N V; Fukazawa, Y; Gomez, J L; Hagen-Thorn, V A; Heidt, J; Hiriart, D; Itoh, R; Joshi, M; Kimeridze, G N; Konstantinova, T S; Kopatskaya, E N; Korobtsev, I V; Kovalev, Y Y; Krajci, T; Kurtanidze, O; Kurtanidze, S O; Larionov, V M; Larionova, E G; Larionova, L V; Lindfors, E; Lopez, J M; Marscher, A P; McHardy, I M; Molina, S N; Morozova, D A; Nazarov, S V; Nikolashvili, M G; Nilsson, K; Pulatova, N G; Reinthal, R; Sadun, A; Sergeev, S G; Sigua, L A; Sorcia, M; Spiridonova, O I; Takalo, L O; Taylor, B; Troitsky, I S; Ugolkova, L S; Zensus, J A; Zhdanova, V E


    Quasar 3C 279 is known to exhibit episodes of optical polarization angle rotation. We present new, well-sampled optical polarization data for 3C 279 and introduce a method to distinguish between random and deterministic electric vector position angle (EVPA) variations. We observe EVPA rotations in both directions with different amplitudes and find that the EVPA variation shows characteristics of both random and deterministic cases. Our analysis indicates that the EVPA variation is likely dominated by a random process in the low brightness state of the jet and by a deterministic process in the flaring state.

  9. L3+C air shower array

    Laurent Guiraud


    Photo 01: a view of the L3+C air shower array; 50 scintillators on the roof of the SX-hall above L3. Photo 02: view of one of the detectors of the array.Photo 04: detectors seen against the background of the LEP Point 2 facilities.

  10. Numerical simulation of surface and downhole deformation induced by hydraulic fracturing

    He Yi-Yuan; Zhang Bao-Ping; Duan Yu-Ting; Xue Cheng-Jin; Yan Xin; He Chuan; Hu Tian-Yue


    Tiltmeter mapping technology infers hydraulic fracture geometry by measuring fracture-induced rock deformation, which recorded by highly sensitive tiltmeters placed at the surface and in nearby observation wells. By referencing Okada’s linear elastic theory and Green’s function method, we simulate and analyze the surface and downhole deformation caused by hydraulic fracturing using the homogeneous elastic half-space model and layered elastic model. Simulation results suggest that there is not much difference in the surface deformation patterns between the two models, but there is a significant difference in the downhole deformation patterns when hydraulic fracturing penetrates a stratum. In such cases, it is not suitable to assume uniform elastic half-space when calculating the downhole deformation. This work may improve the accuracy and reliability of the inversion results of tiltmeter monitoring data.

  11. Early Detection and Localization of Downhole Incidents in Managed Pressure Drilling

    Willersrud, Anders; Imsland, Lars; Blanke, Mogens


    Downhole incidents such as kick, lost circulation, pack-off, and hole cleaning issues are important contributors to downtime in drilling. In managed pressure drilling (MPD), operations margins are typically narrower, implying more frequent incidents and more severe consequences. Detection...... and handling of symptoms of downhole drilling contingencies at an early stage are therefore crucial for the reliability and safety of MPD operations. In this paper we describe a method for early detection and localization of such incidents, based on a fit for purpose model of the downhole pressure hydraulics......, these incidents have been success- fully detected at an early stage, when detection by a human operator observing the measured data is almost impossible. The developed detection and localization method can be included as a diagnosis tool in a drilling system with MPD or conventional drilling...

  12. The PCNA-RFC families of DNA clamps and clamp loaders.

    Majka, Jerzy; Burgers, Peter M J


    The proliferating cell nuclear antigen PCNA functions at multiple levels in directing DNA metabolic pathways. Unbound to DNA, PCNA promotes localization of replication factors with a consensus PCNA-binding domain to replication factories. When bound to DNA, PCNA organizes various proteins involved in DNA replication, DNA repair, DNA modification, and chromatin modeling. Its modification by ubiquitin directs the cellular response to DNA damage. The ring-like PCNA homotrimer encircles double-stranded DNA and slides spontaneously across it. Loading of PCNA onto DNA at template-primer junctions is performed in an ATP-dependent process by replication factor C (RFC), a heteropentameric AAA+ protein complex consisting of the Rfc1, Rfc2, Rfc3, Rfc4, and Rfc5 subunits. Loading of yeast PCNA (POL30) is mechanistically distinct from analogous processes in E. coli (beta subunit by the gamma complex) and bacteriophage T4 (gp45 by gp44/62). Multiple stepwise ATP-binding events to RFC are required to load PCNA onto primed DNA. This stepwise mechanism should permit editing of this process at individual steps and allow for divergence of the default process into more specialized modes. Indeed, alternative RFC complexes consisting of the small RFC subunits together with an alternative Rfc1-like subunit have been identified. A complex required for the DNA damage checkpoint contains the Rad24 subunit, a complex required for sister chromatid cohesion contains the Ctf18 subunit, and a complex that aids in genome stability contains the Elg1 subunit. Only the RFC-Rad24 complex has a known associated clamp, a heterotrimeric complex consisting of Rad17, Mec3, and Ddc1. The other putative clamp loaders could either act on clamps yet to be identified or act on the two known clamps.

  13. Multifrequency observations of extended radio galaxies V - 3C 31, 3C 33.1, 3C 35, 3C 66B, 3C 129, 3C 130, 3C 223, 3C 310, 3C 390.3 and 4C 48.29

    van Breugel, W.; Jagers, W.


    A sample of 3C radio sources of large angular size has been observed in total and polarized intensity at several wavelengths with the Westerbork Synthesis Radio Telescope. The sources were selected such that their largest angular size was greater than about 200 arcsec and their declination greater than about 25 degrees. Some additional sources with radio jets or peculiar morphology were also included. The name of each source, its structural type classification, wavelength of observation, and data references are given.

  14. Advanced patch-clamp techniques and single-channel analysis

    Biskup, B; Elzenga, JTM; Homann, U; Thiel, G; Wissing, F; Maathuis, FJM


    Much of our knowledge of ion-transport mechanisms in plant cell membranes comes from experiments using voltage-clamp. This technique allows the measurement of ionic currents across the membrane, whilst the voltage is held under experimental control. The patch-clamp technique was developed to study t

  15. Applications of alcohol clamping in early drug development

    Zoethout, Remco Wiebe Martijn


    This thesis describes the development of a novel alcohol clamp, a new method to obtain stable plasma levels of alcohol and its application in CNS-research. The method might have several advantages that were explored in subsequent studies described in this thesis. The stability of the alcohol clamp w

  16. New active load voltage clamp for HF-link converters

    Ljusev, Petar; Andersen, Michael Andreas E.


    This paper proposes a new active clamp for HF-link converters, which features very high efficiency by returning the clamped energy back to the primary side through a small auxiliary converter. It also increases the reliability of HF-link converters by providing an alternative load current path du...

  17. Studying mechanosensitive ion channels with an automated patch clamp

    Barthmes, Maria; Jose, Mac Donald F; Birkner, Jan Peter; Brüggemann, Andrea; Wahl-Schott, Christian; Kocer, Armagan


    Patch clamp electrophysiology is the main technique to study mechanosensitive ion channels (MSCs), however, conventional patch clamping is laborious and success and output depends on the skills of the operator. Even though automated patch systems solve these problems for other ion channels, they cou

  18. Analysis of Contact Characteristic of Overhead Line and Suspension Clamp

    Zhao Xinze


    Full Text Available In this paper, a LGJ150/25 type ACSR transmission line and a CGU-3 type suspension clamp are taken as research objects. A contact model of the conductor and the clamp was established by using finite element method. The effects of sag angle of the conductor, holding force and tension force in section are analyzed. The results showed that the contact area in the middle of the clamp is of belt-like type. The extreme values of tress were observed on the edge of the contact area and near the edge of keeper. In clamp section, suspension angle had the greatest influence on contact stress, and then the clamp force. The tension force in section played a most important role in these affecting factors. In the exit section of clamp, the biggest impact factor was tension force in this section, then the suspension angle, the third was clamp force. The results provide theoretical basis on reducing corona loss, optimization the clamp. Doubtlessly, the conclusion has important theoretical significance and application value.

  19. 21 CFR 876.5160 - Urological clamp for males.


    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Urological clamp for males. 876.5160 Section 876.5160 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES GASTROENTEROLOGY-UROLOGY DEVICES Therapeutic Devices § 876.5160 Urological clamp for...

  20. Feasibility evaluation of downhole oil/water separator (DOWS) technology.

    Veil, J. A.; Langhus, B. G.; Belieu, S.


    The largest volume waste stream associated with oil and gas production is produced water. A survey conducted by the American Petroleum Institute estimated that 20.9 billion barrels of produced water were disposed of in 1985 (Wakim 1987). Of this total, 91% was disposed of through disposal wells or was injected for enhanced oil recovery projects. Treatment and disposal of produced water represents a significant cost for operators. A relatively new technology, downhole oil/water separators (DOWS), has been developed to reduce the cost of handling produced water. DOWS separate oil and gas from produced water at the bottom of the well and reinject some of the produced water into another formation or another horizon within the same formation, while the oil and gas are pumped to the surface. Since much of the produced water is not pumped to the surface, treated, and pumped from the surface back into a deep formation, the cost of handling produced water is greatly reduced. When DOWS are used, additional oil may be recovered as well. In cases where surface processing or disposal capacity is a limiting factor for further production within a field, the use of DOWS to dispose of some of the produced water can allow additional production within that field. Simultaneous injection using DOWS minimizes the opportunity for contamination of underground sources of drinking water (USDWs) through leaks in tubing and casing during the injection process. This report uses the acronym 'DOWS' although the technology may also be referred to as DHOWS or as dual injection and lifting systems (DIALS). Simultaneous injection using DOWS has the potential to profoundly influence the domestic oil industry. The technology has been shown to work in limited oil field applications in the United States and Canada. Several technical papers describing DOWS have been presented at oil and gas industry conferences, but for the most part, the information on the DOWS technology has not been widely

  1. A downhole passive sampling system to avoid bias and error from groundwater sample handling.

    Britt, Sanford L; Parker, Beth L; Cherry, John A


    A new downhole groundwater sampler reduces bias and error due to sample handling and exposure while introducing minimal disturbance to natural flow conditions in the formation and well. This "In Situ Sealed", "ISS", or "Snap" sampling device includes removable/lab-ready sample bottles, a sampler device to hold double end-opening sample bottles in an open position, and a line for lowering the sampler system and triggering closure of the bottles downhole. Before deployment, each bottle is set open at both ends to allow flow-through during installation and equilibration downhole. Bottles are triggered to close downhole without well purging; the method is therefore "passive" or "nonpurge". The sample is retrieved in a sealed condition and remains unexposed until analysis. Data from six field studies comparing ISS sampling with traditional methods indicate ISS samples typically yield higher volatile organic compound (VOC) concentrations; in one case, significant chemical-specific differentials between sampling methods were discernible. For arsenic, filtered and unfiltered purge results were negatively and positively biased, respectively, compared to ISS results. Inorganic constituents showed parity with traditional methods. Overall, the ISS is versatile, avoids low VOC recovery bias, and enhances reproducibility while avoiding sampling complexity and purge water disposal.

  2. Soft-sensing for multilateral wells with downhole pressure and temperature measurements

    Kruif, B.J. de; Leskens, M.; Linden, R. van der; Alberts, G.J.N.


    Real-time monitoring of downhole oil, gas and water flows in wells can significantly improve the production performance of these wells when this flow rate information is used to manipulate inflow control valves. An example of this is the allocation of a gas or water cone to its entrance point in a m

  3. Downhole multiphase metering in wells by means of soft-sensing

    Leskens, M.; Kruif, B. de; Belfroid, S.P.C.; Smeulers, J.P.M.; Gryzlov, A.


    Multiphase flow meters are indispensable tools for achieving optimal operation and control of wells as these meters deliver real-time information about their performance. For example, multiphase flow meters located downhole can improve the production of multilateral and multizone wells by timely all

  4. Delay Pressure Detection Method to Eliminate Pump Pressure Interference on the Downhole Mud Pressure Signals

    Yue Shen


    Full Text Available The feasibility of applying delay pressure detection method to eliminate mud pump pressure interference on the downhole mud pressure signals is studied. Two pressure sensors mounted on the mud pipe in some distance apart are provided to detect the downhole mud continuous pressure wave signals on the surface according to the delayed time produced by mud pressure wave transmitting between the two sensors. A mathematical model of delay pressure detection is built by analysis of transmission path between mud pump pressure interference and downhole mud pressure signals. Considering pressure signal transmission characteristics of the mud pipe, a mathematical model of ideal low-pass filter for limited frequency band signal is introduced to study the pole frequency impact on the signal reconstruction and the constraints of pressure sensor distance are obtained by pole frequencies analysis. Theoretical calculation and numerical simulation show that the method can effectively eliminate mud pump pressure interference and the downhole mud continuous pressure wave signals can be reconstructed successfully with a significant improvement in signal-to-noise ratio (SNR in the condition of satisfying the constraints of pressure sensor distance.

  5. Cell-Detection Technique for Automated Patch Clamping

    McDowell, Mark; Gray, Elizabeth


    A unique and customizable machinevision and image-data-processing technique has been developed for use in automated identification of cells that are optimal for patch clamping. [Patch clamping (in which patch electrodes are pressed against cell membranes) is an electrophysiological technique widely applied for the study of ion channels, and of membrane proteins that regulate the flow of ions across the membranes. Patch clamping is used in many biological research fields such as neurobiology, pharmacology, and molecular biology.] While there exist several hardware techniques for automated patch clamping of cells, very few of those techniques incorporate machine vision for locating cells that are ideal subjects for patch clamping. In contrast, the present technique is embodied in a machine-vision algorithm that, in practical application, enables the user to identify good and bad cells for patch clamping in an image captured by a charge-coupled-device (CCD) camera attached to a microscope, within a processing time of one second. Hence, the present technique can save time, thereby increasing efficiency and reducing cost. The present technique involves the utilization of cell-feature metrics to accurately make decisions on the degree to which individual cells are "good" or "bad" candidates for patch clamping. These metrics include position coordinates (x,y) in the image plane, major-axis length, minor-axis length, area, elongation, roundness, smoothness, angle of orientation, and degree of inclusion in the field of view. The present technique does not require any special hardware beyond commercially available, off-the-shelf patch-clamping hardware: A standard patchclamping microscope system with an attached CCD camera, a personal computer with an imagedata- processing board, and some experience in utilizing imagedata- processing software are all that are needed. A cell image is first captured by the microscope CCD camera and image-data-processing board, then the image

  6. ALMA Polarization Science Verification: 3C 286

    Nagai, H.; Nakanishi, K.; Paladino, R.; Moellenbrock, G.; Fomalont, E.; Amigano, A.; Vlahakis, C.; Remijan, A.; ALMA Polarization Team


    The ALMA polarization science verification results on 3C 286 are presented. The measured polarization percentage and polarization position angle of the continuum emission at 1.3 mm are about 16% and 39 degrees, respectively. They are quite similar to those at longer wavelength, but seem to increase slightly. Similar trends were also found in the previous measurement using the IRAM 30-m telescope (Agudo et al. 2012). The final image rms on the polarization image is better than 0.1% of the total intensity, demonstrating the very high polarization sensitivity of ALMA.

  7. Trends in Aortic Clamp Use During Coronary Artery Bypass Surgery: The Effect of Aortic Clamping Strategies on Neurologic Outcomes

    Daniel, William T.; Kilgo, Patrick; Puskas, John D.; Thourani, Vinod H.; Lattouf, Omar M.; Guyton, Robert A.; Halkos, Michael E.


    Objective The purpose of this study was to determine the impact of different clamping strategies during CABG on the incidence of postoperative stroke. Methods In this case-control study, all patients at Emory hospitals from 2002–2009 with postoperative stroke after isolated CABG (N=141) were matched 1:4 to a contemporaneous cohort of patients without postoperative stroke (N=565). Patients were matched according to the Society of Thoracic Surgeons (STS) Predicted Risk of Postoperative Stroke (PROPS), which is based on 26 variables. On- (ONCAB) and off-pump (OPCAB) patients were matched separately. Multiple logistic regression analysis with adjusted odds ratios (OR) was performed to identify operative variables associated with postoperative stroke. Results Among the ONCAB cohort, the single cross-clamp technique was associated with a decreased risk of stroke compared to the double clamp (cross clamp + partial clamp) technique (OR=0.385, p=0.044). Within the OPCAB cohort, there was no significant difference in stroke according to clamp use. Epiaortic ultrasound of the ascending aorta increased from 45.3% in 2002 to 89.4% in 2009. From 2002–2009, clamp use decreased from 97.7% of cases to 72.7%. Conclusions During ONCAB, the use of a single cross-clamp compared to the double clamp technique decreases the risk of postoperative stroke. The use of any aortic clamp has decreased and epiaortic ultrasound use has increased from 2002–2009, indicating a change in operative technique and surgeon awareness of the potential complications associated with manipulation of the aorta. PMID:23477689

  8. Single electrode dynamic clamp with StdpC.

    Samu, David; Marra, Vincenzo; Kemenes, Ildiko; Crossley, Michael; Kemenes, György; Staras, Kevin; Nowotny, Thomas


    Dynamic clamp is a powerful approach for electrophysiological investigations allowing researchers to introduce artificial electrical components into target neurons to simulate ionic conductances, chemical or electrotonic inputs or connections to other cells. Due to the rapidly changing and potentially large current injections during dynamic clamp, problematic voltage artifacts appear on the electrode used to inject dynamic clamp currents into a target neuron. Dynamic clamp experiments, therefore, typically use two separate electrodes in the same cell, one for recording membrane potential and one for injecting currents. The requirement for two independent electrodes has been a limiting factor for the use of dynamic clamp in applications where dual recordings of this kind are difficult or impossible to achieve. The recent development of an active electrode compensation (AEC) method has overcome some of these prior limitations, permitting artifact-free dynamic clamp experimentation with a single electrode. Here we describe an AEC method for the free dynamic clamp software StdpC. The AEC component of StdpC is the first such system implemented for the use of non-expert users and comes with a set of semi-automated configuration and calibration procedures that facilitate its use. We briefly introduce the AEC method and its implementation in StdpC and then validate it with an electronic model cell and in two different biological preparations.

  9. The multiwavelength variability of 3C 273

    Soldi, S; Paltani, S; Aller, H D; Aller, M F; Bürki, G; Chernyakova, M; Lähteenmäki, A; McHardy, I M; Robson, E I; Staubert, R; Tornikoski, M; Walter, R; Courvoisier, T J -L


    We present an update of 3C 273's database hosted by the ISDC, completed with data from radio to gamma-ray observations over the last 10 years. We use this large data set to study the multiwavelength properties of this quasar,especially focussing on its variability behaviour. We study the amplitude of the variations and the maximum variability time scales across the broad-band spectrum and correlate the light curves in different bands, specifically with the X-rays, to search for possible connections between the emission at different energies. 3C 273 shows variability at all frequencies, with amplitudes and time scales strongly depending on the energy and being the signatures of the different emission mechanisms. The variability properties of the X-ray band imply the presence of either two separate components (possibly a Seyfert-like and a blazar-like) or at least two parameters with distinct timing properties to account for the X-ray emission below and above ~20 keV. The dominant hard X-ray emission is most pr...

  10. Integral transform solutions of dynamic response of a clamped-clamped pipe conveying fluid

    Gu Jijun; An Chen [Ocean Engineering Program, COPPE, Universidade Federal do Rio de Janeiro, CP 68508, Rio de Janeiro 21941-972 (Brazil); Duan Menglan [Offshore Oil/Gas Research Center, China University of Petroleum, Beijing 102249 (China); Levi, Carlos [Ocean Engineering Program, COPPE, Universidade Federal do Rio de Janeiro, CP 68508, Rio de Janeiro 21941-972 (Brazil); Su Jian, E-mail: [Nuclear Engineering Program, COPPE, Universidade Federal do Rio de Janeiro, CP 68509, Rio de Janeiro 21941-972 (Brazil)


    Highlights: Black-Right-Pointing-Pointer Dynamic response of pipe conveying fluid was studied numerically. Black-Right-Pointing-Pointer The generalized integral transform technique (GITT) was applied. Black-Right-Pointing-Pointer Numerical solutions with automatic global accuracy control were obtained. Black-Right-Pointing-Pointer Excellent convergence behavior was shown. Black-Right-Pointing-Pointer Modal separation analysis was carried out and the influence of mass ratio was analyzed. - Abstract: Analysis of dynamic response of pipe conveying fluid is an important aspect in nuclear power plant design. In the present paper, dynamic response of a clamped-clamped pipe conveying fluid was solved by the generalized integral transform technique (GITT). The governing partial differential equation was transformed into a set of second-order ordinary differential equations which is then numerically solved by making use of the subroutine DIVPAG from IMSL Library. A thorough convergence analysis was performed to yield sets of reference results of the transverse deflection at different time and spanwise position. We found good agreement between the computed natural frequencies at mode 1-3 and those obtained by previous theoretical study. Besides, modal separation analysis was carried out and the influence of mass ratio on deflection and natural frequencies was qualitatively and quantitatively assessed.

  11. Planar patch-clamp force microscopy on living cells

    Pamir, Evren [Center for Nano Science, Ludwig-Maximilians University, Amalienstr 54, 80799 Munich (Germany); George, Michael; Fertig, Niels [Nanion Technologies GmbH, Erzgiessereistr. 4, 80335 Munich (Germany); Benoit, Martin [Center for Nano Science, Ludwig-Maximilians University, Amalienstr 54, 80799 Munich (Germany)], E-mail:


    Here we report a new combination of the patch-clamp technique with the atomic force microscope (AFM). A planar patch-clamp chip microstructured from borosilicate glass was used as a support for mechanical probing of living cells. The setup not only allows for immobilizing even a non-adherent cell for measurements of its mechanical properties, but also for simultaneously measuring the electrophysiological properties of a single cell. As a proof of principle experiment we measured the voltage-induced membrane movement of HEK293 and Jurkat cells in the whole-cell voltage clamp configuration. The results of these measurements are in good agreement with previous studies. By using the planar patch-clamp chip for immobilization, the AFM not only can image non-adhering cells, but also gets easily access to an electrophysiologically controlled cellular probe at low vibrational noise.

  12. Delay in Clamping Umbilical Cord Benefits Babies, Doctors Say

    ... doctors' group said. Although delayed clamping increases the newborn's risk of jaundice that will require treatment, ACOG said there's no evidence that waiting increases the mother's risk of hemorrhage. The guidelines are to be published in the ...

  13. A clamp-like biohybrid catalyst for DNA oxidation

    van Dongen, Stijn F. M.; Clerx, Joost; Nørgaard, Kasper; Bloemberg, Tom G.; Cornelissen, Jeroen J. L. M.; Trakselis, Michael A.; Nelson, Scott W.; Benkovic, Stephen J.; Rowan, Alan E.; Nolte, Roeland J. M.


    In processive catalysis, a catalyst binds to a substrate and remains bound as it performs several consecutive reactions, as exemplified by DNA polymerases. Processivity is essential in nature and is often mediated by a clamp-like structure that physically tethers the catalyst to its (polymeric) template. In the case of the bacteriophage T4 replisome, a dedicated clamp protein acts as a processivity mediator by encircling DNA and subsequently recruiting its polymerase. Here we use this DNA-binding protein to construct a biohybrid catalyst. Conjugation of the clamp protein to a chemical catalyst with sequence-specific oxidation behaviour formed a catalytic clamp that can be loaded onto a DNA plasmid. The catalytic activity of the biohybrid catalyst was visualized using a procedure based on an atomic force microscopy method that detects and spatially locates oxidized sites in DNA. Varying the experimental conditions enabled switching between processive and distributive catalysis and influencing the sliding direction of this rotaxane-like catalyst.

  14. Choosing the wire clamp microdrive for termosonic microwelding equipments

    Lanin V. L.


    Full Text Available The article describes research of three types of wire clamp microdrive for termosonic microwelding equipment, covering an expanded range microconductors from 12,5 up to 75 mm. The most preferable, in the light of their high-speed and easy-programming qualities, proved to be the mechanisms of conductor clamp with piezoelement drives and the loading system based on "voice-coil" type electromagnet.

  15. Prehospital Emergency Inguinal Clamp Controls Hemorrhage in Cadaver Model


    the CRoC preassembled for quick use. The clamp compresses the casualty’s tissues and is unat- tached to things like a litter. When screwed down, it is...for use on the battlefield to control difficult bleeds in the inguinal area. Study Design The study design was a sequence of experiments of clamp...temperature 4 hours before testing. The torso was rotated onto a wood block to intubate the aorta through a left thoracotomy incision. The thoracic aorta

  16. Photometric reverberation mapping of 3C120

    Nuñez, F Pozo; Westhues, C; Bruckmann, C; Haas, M; Chini, R; Steenbrugge, K; Murphy, M; 10.1051/0004-6361/201219107


    We present the results of a five month monitoring campaign of the local active galactic nuclei (AGN) 3C120. Observations with a median sampling of two days were conducted with the robotic 15cm telescope VYSOS-6 located near Cerro Armazones in Chile. Broad band (B,V) and narrow band (NB) filters were used in order to measure fluxes of the AGN and the H_beta broad line region (BLR) emission line. The NB flux is constituted by about 50% continuum and 50% H_beta emission line. To disentangle line and continuum flux, a synthetic H_beta light curve was created by subtracting a scaled V-band light curve from the NB light curve. Here we show that the H_beta emission line responds to continuum variations with a rest frame lag of 23.6 +/- 1.69 days. We estimate a virial mass of the central black hole M_BH = 57 +/- 27 * 10^6 solar masses, by combining the obtained lag with the velocity dispersion of a single contemporaneous spectrum. Using the flux variation gradient (FVG) method, we determined the host galaxy subtracte...

  17. Low-frequency study of two giant radio galaxies: 3C 35 and 3C 223

    Orrù, E.; Murgia, M.; Feretti, L.; Govoni, F.; Giovannini, G.; Lane, W.; Kassim, N.; Paladino, R.


    Aims: Radio galaxies with a projected linear size ⪆1 Mpc are classified as giant radio sources. According to the current interpretation these are old sources which have evolved in a low-density ambient medium. Because radiative losses are negligible at low frequency, extending spectral aging studies in this frequency range will allow us to determine the zero-age electron spectrum injected and then to improve the estimate of the synchrotron age of the source. Methods: We present Very Large Array images at 74 MHz and 327 MHz of two giant radio sources: 3C 35 and 3C 223. We performed a spectral study using 74, 327, 608 and 1400 GHz images. The spectral shape is estimated in different positions along the source. Results: The radio spectrum follows a power-law in the hotspots, while in the inner region of the lobe the shape of the spectrum shows a curvature at high frequencies. This steepening agrees with synchrotron aging of the emitting relativistic electrons. In order to estimate the synchrotron age of the sources, the spectra were fitted with a synchrotron model of emission. They show that 3C 35 is an old source of 143 ± 20 Myr, while 3C 223 is a younger source of 72 ± 4 Myr.

  18. Numerical Study of Water Control with Downhole Oil-Water Separation Technology

    Yin Khor Yin


    Full Text Available The maturing oil fields with increasing water production can pose a challenging produced water handling and disposal issues. This paper presents a numerical study of a motorless hydrocyclone to enhance understanding of the downhole oil-water separation. The turbulence of fluid flow is obtained using K-ε Realizable Turbulence model for complex swirl dominated flow, while the interface between hydrocarbon and water is described using the Discrete Phase model. In this approach, factors which contribute to the hydrocyclone separation instability were discussed. Discussion is then extended to the relationship of residence time with pressure difference between overflow and underflow. These pressure differences are able to relate to pressure condition for high water cut well which require downhole separation.

  19. Sonde for Downhole Measurement of Water Turbidity and Dye Tracer Concentration

    Schnegg, Pierre-André; Bossy, F.


    A new flow-through field fluorometer sonde has been designed for use in downhole tracer tests in 2’’ boreholes. The instrument is capable of determining the partial concentration of two dye tracers present simultaneously in the water. In addition, turbidity can be measured if the water is free of tracers. Although the sonde is aimed at boreholed hydrological investigations, it can also be used in surface waters.

  20. Back azimuth constrained double-difference seismic location and tomography for downhole microseismic monitoring

    Chen, Yukuan; Zhang, Haijiang; Miao, Yuanyuan; Zhang, Yinsheng; Liu, Qiang


    We have developed a new seismic tomography method, back azimuth constrained double-difference (DD) seismic tomography, which is suitable for downhole microseismic monitoring of hydraulic fracturing. The new method simultaneously locates microseismic events and determines three-dimensional (3D) Vp and Vs models for the fracturing zone using differential arrival times from pairs of events and event back azimuths in addition to absolute arrival times. Compared to the existing DD location and tomography method, our method incorporates back azimuth information to better constrain microseismic event locations in the case of poor spatial station coverage such as the linear downhole seismic array generally used for microseismic monitoring. By incorporating the relative arrival time and back azimuth information of events, the extended DD method can provide better relative event locations, and thus can better characterize the fracture distribution. In addition to microseismic locations, seismic velocity anomalies determined around the fracturing zone may also provide valuable information for fracture development. Due to the existence of fractures and fluids, the seismic velocity is expected to be lower in the fractured zone compared to the surrounding regions. Therefore the area of low seismic velocity anomaly may be used as a proxy for the stimulated reservoir volume. We have applied the new method to a downhole microseismic dataset from shale gas hydraulic fracturing. The microseismic events are more accurately relocated than the conventional grid search location method, and they are generally associated with low velocity anomalies.

  1. Development and Application of the Downhole Drilling String Shock-Absorption and Hydraulic Supercharging Device

    Yongwang Liu


    Full Text Available It is a hot topic for deep/ultradeep wells to improve rock-breaking efficiency and drilling speed by available downhole energy. Based on different downhole energies and working conditions, specialized plunger pump is proposed to convert longitudinal vibration of drilling string into rock-breaking energy. Technical design is developed to generate high-pressure water jet. And then a simulation model is built to verify feasibility of the technical design. Through simulation, the influence law of key factors is obtained. On this basis, this device is tested in several wells. The result indicates this device can increase drilling speed as much as 136%. Meanwhile the harmful vibration can be absorbed. The energy from drilling string vibration is of high frequency and increases as well depth and formation anisotropy increase. By reducing adverse vibration, this device is able to increase the drilling speed and the service life also meets the demand of field application. The longest working time lasts for more than 130 hours. The performance of this device demonstrates great application prospect in deep/ultradeep resources exploration. To provide more equipment support for deep/ultradeep wells, more effort should be put into fundamental study on downhole drill string vibration and related equipment.

  2. FBG sensing temperature characteristic and application in oil/gas down-hole measurement

    Shaomin LI; Xiaoying LIU; You LI; Shenlong YANG; Chong LIU


    Fiber Bragg gratings (FBGs) have been used to sense numerous parameters such as strain, temperature, and pressure. Cost-effective multipoint measurements have been achieved by connecting FBGs in parallel, serial, and other topologies as well as by using spatial, wavelength, and time-domain multiplexing techniques. This paper presents a method of measuring temperature of the oil/gas down-hole. Detailed contents include the basic theory and characteristics of fiber gratings, analysis of the sensing mechanism of fiber-optic gratings, and the cross-sensitivity effect between temperature and strain; the method of making the light-source of the fiber-optic gratings and the technology of measuring wavelength shift, building an experimental system of the temperature measurement, and dealing with the experimental data. The paper makes a comparison of several kinds of FBG sensing systems used in oil/gas down-hole to measure temperature and the analysis of the experimental results of building the temperature measurement experimental sys-tem. It demonstrates that the fiber-optic grating sensing method is the best choice in all methods of measuring temperature in oil/gas down-hole, which has a brilliant applied prospect.

  3. Photosynthesis of C3, C3-C4, and C4 grasses at glacial CO2.

    Pinto, Harshini; Sharwood, Robert E; Tissue, David T; Ghannoum, Oula


    Most physiology comparisons of C3 and C4 plants are made under current or elevated concentrations of atmospheric CO2 which do not reflect the low CO2 environment under which C4 photosynthesis has evolved. Accordingly, photosynthetic nitrogen (PNUE) and water (PWUE) use efficiency, and the activity of the photosynthetic carboxylases [Rubisco and phosphoenolpyruvate carboxylase (PEPC)] and decarboxylases [NADP-malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PEP-CK)] were compared in eight C4 grasses with NAD-ME, PCK, and NADP-ME subtypes, one C3 grass, and one C3-C4 grass grown under ambient (400 μl l(-1)) and glacial (180 μl l(-1)) CO2. Glacial CO2 caused a smaller reduction of photosynthesis and a greater increase of stomatal conductance in C4 relative to C3 and C3-C4 species. Panicum bisulcatum (C3) acclimated to glacial [CO2] by doubling Rubisco activity, while Rubisco was unchanged in Panicum milioides (C3-C4), possibly due to its high leaf N and Rubisco contents. Glacial CO2 up-regulated Rubisco and PEPC activities in concert for several C4 grasses, while NADP-ME and PEP-CK activities were unchanged, reflecting the high control exerted by the carboxylases relative to the decarboxylases on the efficiency of C4 metabolism. Despite having larger stomatal conductance at glacial CO2, C4 species maintained greater PWUE and PNUE relative to C3-C4 and C3 species due to higher photosynthetic rates. Relative to other C4 subtypes, NAD-ME and PEP-CK grasses had the highest PWUE and PNUE, respectively; relative to C3, the C3-C4 grass had higher PWUE and similar PNUE at glacial CO2. Biomass accumulation was reduced by glacial CO2 in the C3 grass relative to the C3-C4 grass, while biomass was less reduced in NAD-ME grasses compared with NADP-ME and PCK grasses. Under glacial CO2, high resource use efficiency offers a key evolutionary advantage for the transition from C3 to C4 photosynthesis in water- and nutrient-limited environments.

  4. The Resolved Outflow from 3C 48

    Shih, Hsin-Yi; Stockton, Alan


    We investigate the properties of the high-velocity outflow driven by the young radio jet of 3C 48, a compact-steep-spectrum source. We use the Space Telescope Imaging Spectrograph on board the Hubble Space Telecope to obtain (1) low-resolution UV and optical spectra and (2) multi-slit medium-resolution spectra of the ionized outflow. With supporting data from ground-based spectrographs, we are able to accurately measure the ratios of diagnostic emission lines such as [O III] λ5007, [O III] λ3727, [N II] λ6548, Hα, Hβ, [Ne V] λ3425, and [Ne III] λ3869. We fit the observed emission-line ratios using a range of ionization models, powered by active galactic nucleus (AGN) radiation and shocks, produced by the MAPPINGS code. We have determined that AGN radiation is likely the dominant ionization source. The outflow's density is estimated to be in the range n = 103-104 cm-3, the mass is ~6 × 106 M ⊙, and the metallicity is likely equal to or higher than solar. Compared with the typical outflows associated with more evolved radio jets, this young outflow is denser, less massive, and more metal rich. Multi-slit observations allow us to construct a two-dimensional velocity map of the outflow that shows a wide range of velocities with distinct velocity components, suggesting a wide-angle clumpy outflow. Based in part on observations made with the NASA/ESA Hubble Space Telescope, obtained at the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS 5-26555. These observations are associated with program GO-11574. Some of the data presented herein were obtained at the W. M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California and the National Aeronautics and Space Administration. The Observatory was made possible by the generous financial support of the W. M. Keck Foundation. Some of the

  5. [Advantage of delayed umbilical cord clamping in the newborn infant].

    Menget, A; Mougey, C; Thiriez, G; Riethmuller, D


    The timing of umbilical cord clamping remains controversial. Although most maternity wards use the early clamping (5-15s), randomized studies and meta-analyses have demonstrated the benefit of delayed clamping for term and preterm newborn infants over the past 10 years. Indeed, placentofetal transfusion of 20-30 ml/kg in 2-3 min improves the iron status of term infants and prevents infant hypochromic anemia. Infant anemia is a public health problem in many developing countries. For preterm newborns, placental transfusion for 45 s or milking the cord for 15 s improves cardiovascular adaptation, with better hemodynamic stability, as well as decreased intraventricular hemorrhages, need for transfusion, and late-onset sepsis. A new look at this symbolic act is needed and professionals need to be persuaded of the importance of the "wait a minute" policy for a better physiological delivery.

  6. Arterial Clamping Increases Central Renal Cryoablation Efficacy: An Animal Study

    Nonboe, Lasse Larsen; Nielsen, Tommy Kjaergaard; Høyer, Søren;


    INTRODUCTION: The minimally invasive treatment of small renal masses with cryoablation has become increasingly widespread during the past 15 years. Studies with long-term follow-up are beginning to emerge, showing good oncological control, however, tumors with a central and endophytic location seem...... to possess an increased risk of treatment failure. Such tumors are likely to be subjected to a high volume of blood giving thermal protection to the cancerous cells. Arterial clamping during freezing might reduce this effect but at the same time subject the kidney to ischemia. The aim of this study...... was to evaluate the effect of renal artery clamping during cryoablation in a porcine survival model. METHODS: Ten Danish Landrace pigs (approximately 40 kg) underwent bilateral laparoscopic cryoablation with clamping of the right renal artery during freezing. The cryoablation consisted of a standard double...

  7. Umbilical cord clamping. An analysis of a usual neonatological conduct.

    Papagno, L


    Here we described a critical analysis of the neonatological procedure of early cord clamping, meaning this, within 40 seconds after birth. Fifty three cases are here analysed, in which this practice was not performed, but instead a late umbilical cord clamping was done after birth or after the cord had stopped beating. Variations in hematocrito values within 24 to 36 hours after birth were studied. A transitory polycithemia, with a maximum peak 12 hours post-delivery was observed. These values returned to normal levels between 24 and 36 hours after birth. K vitamin was not administered to any of the newborns. No pathology appeared related to this transitory polycithemia. In can be concluded that the late umbilical cord clamping represents no risk to the new-born and that the pathological phenomena described under these circumstances may be attributed to the increase in K vitamin dependent coagulation factors that are induced by the routinary administration of phitonadione to all normal newborns.

  8. Plasma temperature clamping in filamentation laser induced breakdown spectroscopy

    Harilal, Sivanandan S.; Yeak, J.; Phillips, Mark C.


    Ultrafast laser filament induced breakdown spectroscopy is a very promising method for remote material detection. We present characteristics of plasmas generated in a metal target by laser filaments in air. Our measurements show that the temperature of the ablation plasma is clamped along the filamentation channel due to intensity clamping in a filament. Nevertheless, significant changes in radiation intensity are noticeable, and this is essentially due to variation in the number density of emitting atoms. The present results also partly explains the reason for the occurrence of atomic plume during fs LIBS in air compared to long-pulse ns LIBS.

  9. New active load voltage clamp for HF-link converters

    Ljusev, P.; Andersen, M.A.E.


    This paper proposes a new active clamp for HF-link converters, which features very high efficiency by returning the clamped energy back to the primary side through a small auxiliary converter. It also increases the reliability of HF-link converters by providing an alternative load current path during malfunctions of the secondary bidirectional bridge. The feasibility of the approach is shown on audio power amplifier prototype. New integrated magnetics design is presented that incorporates both the main power and auxiliary transformer on the same magnetic core. (au)

  10. The problem of isotropic rectangular plate with four clamped edges

    C Erdem İmrak; Ismail Gerdemeli


    The examination of the exact solution of the governing equation of the rectangular plate is important for many reasons. This report discusses in exact solution of the governing equation of an isotropic rectangular plate with four clamped edges. A numerical method for clamped isotropic rectangular plate under distributed loads and an exact solution of the governing equation in terms of trigonometric and hyperbolic function are given. Finally, an illustrative example is given and the results are compared with those reported earlier. This method is found to be easier and effective. The results show reasonable agreement with other available results, but with a simpler and practical approach.

  11. IVC CLAMP: infrahepatic inferior vena cava clamping during hepatectomy - a randomised controlled trial in an interdisciplinary setting

    Reissfelder Christoph


    Full Text Available Abstract Background Intraoperative haemorrhage is a known predictor for perioperative outcome of patients undergoing hepatic resection. While anaesthesiological lowering of central venous pressure (CVP by fluid restriction is known to reduce bleeding during transection of the hepatic parenchyma its potential side effects remain poorly investigated. In theory it may have negative effects on kidney function and tissue perfusion and bears the risk to result in severe haemodynamic instability in case of profound intraoperative blood loss. The present randomised controlled trial evaluates efficacy and safety of infrahepatic inferior vena cava (IVC clamping as an alternative surgical technique to reduce CVP during hepatic resection. Methods/Design The proposed IVC CLAMP trial is a single-centre randomised controlled trial with a two-group parallel design. Patients and outcome-assessors are blinded for the treatment intervention. Patients undergoing elective hepatic resection due to any reason are enrolled in IVC CLAMP. All patients admitted to the Department of General-, Visceral-, and Transplant Surgery, University of Heidelberg for elective hepatic resection are consecutively screened for eligibility and written informed consent is obtained on the day before surgery. The primary objective of this trial is to assess and compare the amount of blood loss during hepatic resection in patients receiving surgical CVP reduction by clamping of the IVC as compared to anaesthesiological CVP without infrahepatic IVC clamping reduction. In addition to blood loss a set of general as well as surgical variables are analysed. Discussion This is a randomised controlled patient and observer blinded two-group parallel trial designed to assess efficacy and safety of infrahepatic IVC clamping during elective hepatectomy. Trial registration ClinicalTrials NCT00732979


    ZhengHuiming; HeZeng; LiMing; ZhaoGaoyu


    This paper investigates how the magnetic constrained layer damping (MCLD) treatment suppresses the displacement and acceleration resonant peak of a clamped-clamped beam. Because MCLI) treatment reduces input work and gives more dissipation energy, the vibration from external excitation can be effectively suppressed.The vibration reduction effects of MCLD and other conventional constrained damping treatments are also evaluated. In many cases, using MCLD treatments can yield smaller displacement and acceleration resonant peak especially in mode 1 compared to the other treatments without greatly changing the natural frequencies of the base beam.

  13. Analysis list: Nr3c1 [Chip-atlas[Archive

    Full Text Available Nr3c1 Adipocyte,Blood,Breast,Embryo,Embryonic fibroblast,Liver,Neural + mm9,,,http




    Full Text Available In the last few years, Indonesian urban growth has tends to be lead to the overground space. This was caused by the significant population increasement the lackness of land availability. The phenomenon is also marked with the increasing number of high-rise buildings and skyscrapers. However, high-rise buildings and skyscrapers have the potential dangers against earthquakes, even more Indonesia lay on an earthquake-prone areas. There are two main things that need to be considered in the planning of earthquake-resistant buildings, which is strength and ductility. Deformation capability and the connection strength between the reinforcement taken into consideration in the design of earthquake-resistant structures. From the steel clamp tensile test results for D13’s mechanical connection reinforcement, it gained 270.69 Mpa for yield strength, 351.45 MPa for ultimate tensile strength and 4757 kg for maximum load with 40% elongation. As for the D16’s mechanical connection reinforcement gained 217.80 MPa for yield strength, 327 605 MPa for ultimate tensile strength and 6717 kg for maximum load with 32% elongation. This study uses two pieces of steel clamp, but in order to obtain a better result then it need to be add more clamps while increasing the steel clamp material quality.

  15. A piezoelectric vibration harvester based on clamped-guided beams

    Wang, Z.; Matova, S.; Elfrink, R.; Jambunathan, M.; Nooijer, C. de; Schaijk, R. van; Vullers, R.J.M.


    The paper addresses the design, modeling, fabrication and experimental results of a piezoelectric energy harvester based on clamped-guided beams. The design is featured by shorter mass displacement and higher reliability than cantilever beams. Two separate sets of capacitors allow exploiting both te

  16. Development and exploitation features of methane-coal wells using downhole pumping equipment

    Kurganova Elena


    Full Text Available In this article extraction of methane from coal beds is presented. The article presents results of the study of downhole pumping equipment used in the exploitation of coal bed methane wells. The features of various types of equipment such as piston sucker-rod pumping units, electrical centrifugal pumping units and sucker-rod screw pumping units have been considered. The results show benefits of sucker-rod screw pumping units because of their versatility and resistance to higher content of mechanical impurities.

  17. Poliovirus protease 3C(pro) kills cells by apoptosis.

    Barco, A; Feduchi, E; Carrasco, L


    The tetracycline-based Tet-Off expression system has been used to analyze the effects of poliovirus protease 3C(pro) on human cells. Stable HeLa cell clones that express this poliovirus protease under the control of an inducible, tightly regulated promoter were obtained. Tetracycline removal induces synthesis of 3C protease, followed by drastic morphological alterations and cellular death. Degradation of cellular DNA in nucleosomes and generation of apoptotic bodies are observed from the second day after 3C(pro) induction. The cleavage of poly(ADP-ribose) polymerase, an enzyme involved in DNA repair, occurs after induction of 3C(pro), indicating caspase activation by this poliovirus protease. The 3C(pro)-induced apoptosis is blocked by the caspase inhibitor z-VAD-fmk. Our findings suggest that the protease 3C is responsible for triggering apoptosis in poliovirus-infected cells by a mechanism that involves caspase activation.

  18. Four- year thyroidectomy procedures: Ligasure Vs. clamp & tie

    Pazoki AR


    Full Text Available "n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: The electrothermal bipolar vessel sealing system is an adjunct to the hemostatic technique, which has been successfully used in many abdominal operation, recently made available to thyroid surgery. The purpose of this was compare the outcomes of new hemostatic technique with traditional method of clamp and tie."n"nMethods: In this retrospective cross-sectional study has been done in surgery's wards of Milad & Rasul- Akram hospitals in Tehran, Iran. During three- year period between 2005-2008 all patients with different kind of pathology that need operation were assessed. Time duration of surgery and hospital stay and post surgery complication in two group, ligasure (n=45 and clamp & tie (n=145 were compared with each others."n"nResults: No difference was seen between two groups in time duration of surgery. Significant difference was seen in hospital stay and complications of surgery. Time of operation in Ligasure group was 132/22 minute and in clamp and tie group was 130/10 minute. There was found significant difference in duration of hospital stay and postoperative complication. Hypocalcemia were seen in two and 24 cases in Ligasure and Clamp & Tie group, respectively (p<0.05. Hospital stay was 2

  19. Advanced Data Communications for Downhole Data Logging and Control Applications in the Oil Industry

    Spracklen, C. T.; Aslam, Tariq


    We present details of 'Mercury', a high-speed downhole communications system that utilizes the (metallic) wall of a gas or oil pipeline or a drill 'string' as the communications 'channel' to control or monitor equipment or sensors used in the oil industry. Conventional downhole communication systems typically use 'mud pulse' telemetry for 'Measurement While Drilling' (MWD) operations. Current mud pulse telemetry technology offers bandwidths of up to 40 bit/s. However the data rate drops with increasing length of the wellbore and is typically as low as 1.5 bit/s - 3.0 bit/s at a depth of 35,000 ft. - 40,000 ft. The system described, by contrast, offers data rates of several megabits per second over distances of many kilometres and uses Orthogonal Frequency Division Multiplexing (OFDM) coupled with Wideband Frequency Division Multiple Access (W-CDMA). This paper presents details of our system; results of several trials undertaken on actual gas pipelines in the UK will be presented at the Conference.

  20. Downhole microseismic monitoring for low signal-to-noise ratio events

    Zhou, Hang; Zhang, Wei; Zhang, Jie


    Microseismic monitoring plays an important role in the process of hydraulic fracturing for shale gas/oil production. The accuracy of event location is an essential issue in microseismic monitoring. The data obtained from downhole monitoring system usually show a higher signal-to-noise ratio (SNR) than the recorded data from the surface. For small microseismic events, however, P waves recorded in a downhole array may be very weak, while S waves are generally dominant and strong. Numerical experiments suggest that inverting S-wave arrival times alone is not sufficient to constrain event locations. In this study, we perform extensive location tests with various noise effects using a grid search method that matches the travel time data of the S wave across a recording array. We conclude that fitting S-wave travel time data along with at least one P-wave travel time of the same event can significantly improve location accuracy. In practice, picking S-wave arrival time data and at least one P-wave pick is possible for many small events. We demonstrate that fitting the combination of the travel time data is a robust approach, which can help increase the number of microseismic events to be located accurately during hydraulic fracturing.

  1. Downhole measurements in the AND-1B borehole, ANDRILL McMurdo Ice Shelf Project, Antarctica

    Morin, R.; Williams, T.; Henrys, S.; Crosby, T.; Hansaraj, D.


    A comprehensive set of downhole measurements was collected in the AND-1B drillhole as part of the on-ice scientific programme defined for the McMurdo Ice Shelf (MIS) Project. Geophysical logs were recorded over two operation phases and consisted of calliper, temperature, fluid conductivity, induction resistivity, magnetic susceptibility, natural gamma activity, acoustic televiewer, borehole deviation, and dipmeter. In addition, two standard vertical seismic profiles (VSP) and one walk-away VSP were obtained. Radioactive logs (porosity and density) were not run because of unstable borehole conditions. Although the total depth of the hole is 1285 metres below seafloor (mbsf), the depth range for in situ measurements was limited by the length of the wireline (1018 mbsf) and by the nullification of some geophysical logs due to the presence of steel casing. A depth correction was derived to account for systematic discrepancies in depth between downhole measurements and cores; consequently, log responses can be directly compared to core properties. The resulting data are amenable to studies of cyclicity and climate, heat flux and fluid flow, and stricture and stress. When integrated with physical properties and fractures measured on the core, this information should play a significant role in addressing many of the scientific objectives of the ANDRILL programme.

  2. Discovery of the Eureka volcanogenic massive sulphide lens using downhole electromagnetics

    Paggi, Jacob; Macklin, Daniel


    The Eureka volcanogenic massive sulphide (VMS) lens forms part of the Stockman Project in north-east Victoria. Eureka was discovered in early 2013, heralding the first new VMS mineralised zone to be discovered at the project since 1979. Key drivers to the detection of Eureka included the combination of downhole electromagnetics (EM) and a robust geological model. The lens is located 350 m to the north-east of the Currawong deposit, at a vertical depth of 360 m. Surface EM methods played a significant role in the discovery of the nearby Currawong and Wilga deposits during the late 1970s. Despite this, modern day airborne and fixed-loop transient EM (FLTEM) surveys failed to detect Eureka, most likely due to its depth, moderate conductance and loop-edge effects masking anomalies. The key component in discovering the lens was the interpretation of two subtle downhole transient electromagnetics (DHTEM) responses from 2012 exploration drillholes. These responses were further strengthened by structural and short wave infrared modelling, presenting a compelling multi-component drill target. The lens was discovered soon thereafter, with a discovery intercept of 22.65 m at 1.2% Cu, 0.7% Pb, 3.9% Zn, 43 g/t Ag and 1.3 g/t Au.

  3. Gigaseal formation in patch clamping with applications of nanotechnology

    Malboubi, Majid


    This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of g...

  4. A new configuration for multilevel converters with diode clamped topology

    Nami, A.; Zare, F.; Ledwich, G.


    of load changes which can avoid neutral point balancing problem in such converters. In addition, the presented topology is suitable for renewable energy systems to boost the low rectified output-voltage. In order to verify the proposed topology, steady state analysis, modelling and simulations are carried......Due to the increased use of renewable energy and power elctronic applications, more multilevel converters (MLC) are developed. A Neutral Point Clamped (NPC) inverter is one of the most used multilevel topologies for wind turbine (WT) and photovoltaic (PV) applications. One of the most crucial...... points in this type of converter is dc-voltage control. In this paper, a novel multi output dc-dc converter connected to a diode clamped topology is presented. This converter, for a given duty cycles, is able to regulate the capacitor voltage to provide an appropriate input voltage for NPC regardless...

  5. Indentation metrology of clamped, ultra-thin elastic sheets

    Vella, Dominic; Davidovitch, Benny


    We study the indentation of ultrathin elastic sheets clamped to the edge of a circular hole. This classical setup has received considerable attention lately, being used by various experimental groups as a probe to measure the surface properties and stretching modulus of thin solid films. Despite the apparent simplicity of this method, the geometric nonlinearity inherent in the mechanical response of thin solid objects renders the analysis of the resulting data a nontrivial task. Importantly, ...

  6. Complement C3c as a Biomarker in Heart Failure

    A. Frey


    Full Text Available Introduction. Experimental data indicates an important role of the innate immune system in cardiac remodeling and heart failure (HF. Complement is a central effector pathway of the innate immune system. Animals lacking parts of the complement system are protected from adverse remodeling. Based on these data, we hypothesized that peripheral complement levels could be a good marker for adverse remodeling and prognosis in patients with HF. Methods and Results. Since complement activation converges on the complement factor C3, we measured serum C3c, a stable C3-conversion product, in 197 patients with stable systolic HF. Subgroups with normal and elevated C3c levels were compared. C3c levels were elevated in 17% of the cohort. Patients with elevated C3c levels exhibited a trend to better survival, slightly higher LVEF, and lower NTpro-BNP values in comparison to patients with normal C3c values. No differences were found regarding NYHA functional class. Significantly more patients with elevated C3c had preexisting diabetes. The prevalence of CAD, arterial hypertension, and atrial fibrillation was not increased in patients with elevated C3c. Conclusion. Elevated C3c levels are associated with less adverse remodeling and improved survival in patients with stable systolic heart failure.

  7. Chaotic Feature in the Light Curve of 3C 273

    Lei Liu


    Some nonlinear dynamical techniques, including state-space reconstruction and correlation integral, are used to analyze the light curve of 3C 273. The result is compared with a chaotic model. The similarities between them suggest there is a low-dimension chaotic attractor in the light curve of 3C 273.

  8. Cleaning patch-clamp pipettes for immediate reuse

    Kolb, I.; Stoy, W. A.; Rousseau, E. B.; Moody, O. A.; Jenkins, A.; Forest, C. R.


    Patch-clamp recording has enabled single-cell electrical, morphological and genetic studies at unparalleled resolution. Yet it remains a laborious and low-throughput technique, making it largely impractical for large-scale measurements such as cell type and connectivity characterization of neurons in the brain. Specifically, the technique is critically limited by the ubiquitous practice of manually replacing patch-clamp pipettes after each recording. To circumvent this limitation, we developed a simple, fast, and automated method for cleaning glass pipette electrodes that enables their reuse within one minute. By immersing pipette tips into Alconox, a commercially-available detergent, followed by rinsing, we were able to reuse pipettes 10 times with no degradation in signal fidelity, in experimental preparations ranging from human embryonic kidney cells to neurons in culture, slices, and in vivo. Undetectable trace amounts of Alconox remaining in the pipette after cleaning did not affect ion channel pharmacology. We demonstrate the utility of pipette cleaning by developing the first robot to perform sequential patch-clamp recordings in cell culture and in vivo without a human operator. PMID:27725751

  9. File list: Oth.ALL.10.Clamp.AllCell [Chip-atlas[Archive

    Full Text Available Oth.ALL.10.Clamp.AllCell dm3 TFs and others Clamp All cell types SRX159170,SRX15917...2,SRX159174,SRX159166,SRX159173 ...

  10. File list: Oth.ALL.05.Clamp.AllCell [Chip-atlas[Archive

    Full Text Available Oth.ALL.05.Clamp.AllCell dm3 TFs and others Clamp All cell types SRX159172,SRX15917...0,SRX159174,SRX159166,SRX159173 ...

  11. File list: Oth.CeL.10.Clamp.AllCell [Chip-atlas[Archive

    Full Text Available Oth.CeL.10.Clamp.AllCell dm3 TFs and others Clamp Cell line SRX159170,SRX159172,SRX...159174,SRX159166,SRX159173 ...

  12. File list: Oth.CeL.20.Clamp.AllCell [Chip-atlas[Archive

    Full Text Available Oth.CeL.20.Clamp.AllCell dm3 TFs and others Clamp Cell line SRX159172,SRX159170,SRX...159174,SRX159166,SRX159173 ...

  13. File list: Oth.ALL.50.Clamp.AllCell [Chip-atlas[Archive

    Full Text Available Oth.ALL.50.Clamp.AllCell dm3 TFs and others Clamp All cell types SRX159172,SRX15917...0,SRX159174,SRX159166,SRX159173 ...

  14. File list: Oth.ALL.20.Clamp.AllCell [Chip-atlas[Archive

    Full Text Available Oth.ALL.20.Clamp.AllCell dm3 TFs and others Clamp All cell types SRX159172,SRX15917...0,SRX159174,SRX159166,SRX159173 ...

  15. File list: Oth.CeL.05.Clamp.AllCell [Chip-atlas[Archive

    Full Text Available Oth.CeL.05.Clamp.AllCell dm3 TFs and others Clamp Cell line SRX159172,SRX159170,SRX...159174,SRX159166,SRX159173 ...

  16. MEMS-based clamp with a passive hold function for precision position retaining of micro manipulators

    Brouwer, D.M.; Jong, de B.R.; Boer, de M.J.; Jansen, H.V.; Dijk, van J.; Krijnen, G.J.M.; Soemers, H.M.J.R.


    In this paper the design, modeling and fabrication of a precision MEMS-based clamp with a relatively large clamping force are presented. The purpose of the clamp is to mechanically fix a six-degree-of-freedom (DOF) MEMS-based sample manipulator (Brouwer et al J. Int. Soc. Precis. Eng. Nanotechnol. s

  17. Investigation of the Dynamics of a Clamped-Clamped Microbeam Near the Third Mode Using a Partial Electrode

    Masri, Karim M.


    We present an investigation of the dynamics of a clamped-clamped microbeam excited electrostatically near its third mode. To maximize the response at the third mode, a partial electrode configuration is utilized. A multi-mode Galerkin method is used to develop a reduced order model (ROM) of the beam. A shooting method to find the periodic motion is utilized to generate frequency response curves. The curves show hardenining behavior and dynamic pull-in. We show that the dynamic amplitude of the partial configuration is higher than that of a full electrode configuration. These results are promising for the use of higher-order modes for mass detection and for ultra sensitive resonant sensors.

  18. Effect of Measured Wellhead Parameters and Well Scaling on the Computed Downhole Conditions in Cerro Prieto Wells

    Goyal, K.P.; Miller, C.W.; Lippmann, M.J.


    This paper is devoted to the discussion of the wellbore model and its describing equations, comparison between the computed and measured pressures and the effect of measured wellhead parameters on the downhole pressures in the well. Finally a wellbore model with multiple inside diameters is discussed and the effect of well scaling on the bottom hole pressures is studied.

  19. Evaluation of selected martensitic stainless steels for use in downhole tubular expansion - Results of a laboratory study

    Mack, Robert [Shell International E and P, b.v. Kessler Park 1, Postbus 60, 2280 AB Rijswijk (Netherlands)


    A laboratory program was performed to evaluate the potential of selected martensitic stainless steels for downhole cladding applications. The evaluation of the effects of tubular expansion on mechanical properties, defects, and resistance to environmentally assisted cracking demonstrated that some steels were acceptable for the intended application. The results were used to qualify and select the stainless steel for the intended sweet cladding applications. (authors)

  20. Preliminary analysis of downhole logging data from ICDP Lake Junin drilling Project, Peru

    Pierdominici, Simona; Kück, Jochem; Rodbell, Donald T.; Abbott, Mark B.


    The International Continental Drilling Programm (ICDP) has supported a scientific drilling campaign in Peru during the summer season 2015. The Lake Junin Drilling Project mainly aims at obtaining high-resolution paleoclimate records from lacustrine sediments to reconstruct the history of the continental records covering the glacial-interglacial cycles. Lake Junín is located at 4000 m a.s.l. in the tropical Andes of Peru, and is characterized by a thick (> 125 m) sediment package deposited at a high rate (0.2 to 1.0 mm yr-1). Lake Junín is one of the few lakes in the tropical Andes that predates the maximum extent of glaciation and is in a geomorphic position to record the waxing and waning of glaciers in nearby cordillera, hence making the lake a key site for the investigation of the Quaternary climate evolution in the inner-tropics of the Southern Hemisphere. Continous coring was performed at three sites in overall 11 boreholes on the lake with at least two overlapping boreholes per site to avoid core gaps. The depth of the boreholes varied between approx. 30 m and 110 m depending on the drill site. The core bit had a bit size of 122.6 mm and yielded a core diameter of 85 mm. Upon completion of coring operations downhole geophysical logging was performed in five of the 11 boreholes (1A, 1C, 1D, 2A and 3B) by the Operational Support Group of ICDP. The main objective was to record in-situ the physical properties of the lacustrine sediments of Lake Junin. Downhole logs provide a powerful tool to fill in information at intervals with core gaps and as depth reference for depth matching of the discontinous cores. Furthermore it will be used for the lithological reconstruction and interpretation. The OSG downhole logging comprised total and spectrum gamma ray, magnetic susceptibility, borehole geometry, temperature, and sonic P-wave velocity. Unstable and collapsing borehole walls made it neccessary to carry out logging in several sections instead of in one run. The

  1. Biglycan is a novel binding partner of fibroblast growth factor receptor 3c (FGFR3c) in the human testis

    Winge, S B; Nielsen, J; Jørgensen, Anders


    identified FGF1, FGF2, and FGF9 as the best candidates for natural ligands of FGFR3c in the testis. In addition, we screened non-FGF proteins and found that a proteoglycan biglycan (BGN) contains a sequence homologous to the FGFR3c binding site on FGF1, and is expressed in peritubular cells adjacent to FGFR3......-expressing spermatogonia. Experiments in a cell-free system confirmed that BGN binds to FGFR3c and FGF1. In conclusion, our findings further clarify the complex regulation of FGFR3c in the human testis. We postulate that BGN is a factor secreted by peritubular cells to modulate FGFR3c signaling and thus...

  2. Field test of two high-pressure, direct-contact downhole steam generators. Volume I. Air/diesel system

    Marshall, B.W.


    As a part of the Project DEEP STEAM to develop technology to more efficiently utilize steam for the recovery of heavy oil from deep reservoirs, a field test of a downhole steam generator (DSG) was performed. The DSG burned No. 2 diesel fuel in air and was a direct-contact, high pressure device which mixed the steam with the combustion products and injected the resulting mixture directly into the oil reservoir. The objectives of the test program included demonstration of long-term operation of a DSG, development of operational methods, assessment of the effects of the steam/combustion gases on the reservoir and comparison of this air/diesel DSG with an adjacent oxygen/diesel direct contact generator. Downhole operation of the air/diesel DSG was started in June 1981 and was terminated in late February 1982. During this period two units were placed downhole with the first operating for about 20 days. It was removed, the support systems were slightly modified, and the second one was operated for 106 days. During this latter interval the generator operated for 70% of the time with surface air compressor problems the primary source of the down time. Thermal contact, as evidenced by a temperature increase in the production well casing gases, and an oil production increase were measured in one of the four wells in the air/diesel pattern. Reservoir scrubbing of carbon monoxide was observed, but no conclusive data on scrubbing of SO/sub x/ and NO/sub x/ were obtained. Corrosion of the DSG combustor walls and some other parts of the downhole package were noted. Metallurgical studies have been completed and recommendations made for other materials that are expected to better withstand the downhole combustion environment. 39 figures, 8 tables.

  3. Privacy Issues of the W3C Geolocation API

    Doty, Nick; Wilde, Erik


    The W3C's Geolocation API may rapidly standardize the transmission of location information on the Web, but, in dealing with such sensitive information, it also raises serious privacy concerns. We analyze the manner and extent to which the current W3C Geolocation API provides mechanisms to support privacy. We propose a privacy framework for the consideration of location information and use it to evaluate the W3C Geolocation API, both the specification and its use in the wild, and recommend some modifications to the API as a result of our analysis.

  4. Clamping stiffness and its influence on load distribution between paired internal spinal fixation devices.

    Rohlmann, A; Calisse, J; Bergmann, G; Radvan, J; Mayer, H M


    The load distribution between two internal spinal fixation devices depends, besides other factors, on their stiffness. The stiffness ranges were determined experimentally for the clamps of the AO internal fixator with lateral nut and with posterior nut as well as for the clamps of the SOCON fixator. The stiffness of eight devices each differed by a factor of 3.1 for the clamp with lateral nut, by a factor of 1.5 for the clamp with posterior nut, and by a factor of 1.4 for the clamp of the SOCON fixator. For the AO clamp with lateral nut, the influence of the nut-tightening torque on the stiffness was determined. Using instrumented internal spinal fixation devices mounted to plastic vertebrae and simulating a corpectomy, the load distribution between the implants was measured for different tightening torques. It could be shown that, for the AO internal fixator whose clamps have a lateral nut, a nut-tightening torque > 5 Nm has only a negligible influence on load-sharing between the implants. Tooth damage occurs when the teeth of the clamp body and clamping jaw of the clamp with lateral nut do not gear together exactly, which leads to changes in the clamping stiffness and load-sharing between the two implants.

  5. Harsh-Environment Solid-State Gamma Detector for Down-hole Gas and Oil Exploration

    Peter Sandvik; Stanislav Soloviev; Emad Andarawis; Ho-Young Cha; Jim Rose; Kevin Durocher; Robert Lyons; Bob Pieciuk; Jim Williams; David O' Connor


    The goal of this program was to develop a revolutionary solid-state gamma-ray detector suitable for use in down-hole gas and oil exploration. This advanced detector would employ wide-bandgap semiconductor technology to extend the gamma sensor's temperature capability up to 200 C as well as extended reliability, which significantly exceeds current designs based on photomultiplier tubes. In Phase II, project tasks were focused on optimization of the final APD design, growing and characterizing the full scintillator crystals of the selected composition, arranging the APD device packaging, developing the needed optical coupling between scintillator and APD, and characterizing the combined elements as a full detector system preparing for commercialization. What follows is a summary report from the second 18-month phase of this program.

  6. Theoretical background and the flow fields in downhole liquid-liquid hydrocyclone (LLHC

    Osei Harrison


    Full Text Available Hydrocyclone system for downhole oil-water separation provides an effective technique of enhancing the economic viability of higher water-cut wells while at the same time reducing the risk of environmental pollution. This paper describes the hydrodynamics of the liquid-liquid hydrocyclones and the flow fields within it are paramount for achieving successful separation process. Some of the important hydrodynamic flow phenomenon within the liquid-liquid hydrocyclone and how they influence the separation efficiency of water/oil was analyzed through analytical solution. The properties of the liquids were based on Bayan offshore field measured properties. The results indicated that there are two swirling zones separated by stagnant flow field. The inner is the light liquid zone, while the outer is the heavy liquid zone.


    Robert Westermark; J. Ford Brett


    This Final Report covers the entire project from July 13, 2000 to June 30, 2003. The report summarizes the details of the work done on the project entitled ''Enhanced Oil Recovery with Downhole Vibration Stimulation in Osage County Oklahoma'' under DOE Contract Number DE-FG26-00BC15191. The project was divided into nine separate tasks. This report is written in an effort to document the lessons learned during the completion of each task. Therefore each task will be discussed as the work evolved for that task throughout the duration of the project. Most of the tasks are being worked on simultaneously, but certain tasks were dependent on earlier tasks being completed. During the three years of project activities, twelve quarterly technical reports were submitted for the project. Many individual topic and task specific reports were included as appendices in the quarterly reports. Ten of these reports have been included as appendices to this final report. Two technical papers, which were written and accepted by the Society of Petroleum Engineers, have also been included as appendices. The three primary goals of the project were to build a downhole vibration tool (DHVT) to be installed in seven inch casing, conduct a field test of vibration stimulation in a mature waterflooded field and evaluate the effects of the vibration on both the produced fluid characteristics and injection well performance. The field test results are as follows: In Phase I of the field test the DHVT performed exceeding well, generating strong clean signals on command and as designed. During this phase Lawrence Berkeley National Laboratory had installed downhole geophones and hydrophones to monitor the signal generated by the downhole vibrator. The signals recorded were strong and clear. Phase II was planned to be ninety-day reservoir stimulation field test. This portion of the field tests was abruptly ended after one week of operations, when the DHVT became stuck in the well

  8. Down-Hole Heat Exchangers: Modelling of a Low-Enthalpy Geothermal System for District Heating

    M. Carlini


    Full Text Available In order to face the growing energy demands, renewable energy sources can provide an alternative to fossil fuels. Thus, low-enthalpy geothermal plants may play a fundamental role in those areas—such as the Province of Viterbo—where shallow groundwater basins occur and conventional geothermal plants cannot be developed. This may lead to being fuelled by locally available sources. The aim of the present paper is to exploit the heat coming from a low-enthalpy geothermal system. The experimental plant consists in a down-hole heat exchanger for civil purposes and can supply thermal needs by district heating. An implementation in MATLAB environment is provided in order to develop a mathematical model. As a consequence, the amount of withdrawable heat can be successfully calculated.

  9. The application limits of alloyed 13%Cr tubular steels for downhole duties

    Cooling, P.J.; Kennani, M.B.; Martin, J.W. [BP Exploration, Sunbury on Thames (United Kingdom); Nice, P.I. [Statoil, Stavanger (Norway)


    In the past few years, a new family of materials has entered the downhole tubular market, known as the ``alloyed 13%Cr steels.`` These are a development of the conventional API 5CT grade 13%Cr steels to which alloying elements of Ni, Mo and Cu have been added, hence offering a number of advantages. In the present paper, domains of application for the 95ksi grade alloyed 13%Cr steels with respect to sour service conditions have been defined on pH vs. H{sub 2}S partial pressure plots. Corrosion properties have also been determined for two other aspects; chloride stress corrosion cracking (ClSCC) and high temperature corrosion resistance. The results have reiterated the importance of solution chemistry, pH and H{sub 2}S partial pressure in defining the corrosion properties of steels.

  10. Fault diagnosis of downhole drilling incidents using adaptive observers and statistical change detection

    Willersrud, Anders; Blanke, Mogens; Imsland, Lars


    Downhole abnormal incidents during oil and gas drilling causes costly delays, any may also potentially lead to dangerous scenarios. Dierent incidents willcause changes to dierent parts of the physics of the process. Estimating thechanges in physical parameters, and correlating these with changes...... expectedfrom various defects, can be used to diagnose faults while in development.This paper shows how estimated friction parameters and ow rates can de-tect and isolate the type of incident, as well as isolating the position of adefect. Estimates are shown to be subjected to non......-Gaussian,t-distributednoise, and a dedicated multivariate statistical change detection approach isused that detects and isolates faults by detecting simultaneous changes inestimated parameters and ow rates. The properties of the multivariate di-agnosis method are analyzed, and it is shown how detection and false alarmprobabilities...

  11. Downhole Microseismic Monitoring at a Carbon Capture, Utilization, and Storage Site, Farnsworth Unit, Ochiltree County, Texas

    Ziegler, A.; Balch, R. S.; van Wijk, J.


    Farnsworth Oil Field in North Texas hosts an ongoing carbon capture, utilization, and storage project. This study is focused on passive seismic monitoring at the carbon injection site to measure, locate, and catalog any induced seismic events. A Geometrics Geode system is being utilized for continuous recording of the passive seismic downhole bore array in a monitoring well. The array consists of 3-component dual Geospace OMNI-2400 15Hz geophones with a vertical spacing of 30.5m. Downhole temperature and pressure are also monitored. Seismic data is recorded continuously and is produced at a rate of over 900GB per month, which must be archived and reviewed. A Short Term Average/Long Term Average (STA/LTA) algorithm was evaluated for its ability to search for events, including identification and quantification of any false positive events. It was determined that the algorithm was not appropriate for event detection with the background level of noise at the field site and for the recording equipment as configured. Alternatives are being investigated. The final intended outcome of the passive seismic monitoring is to mine the continuous database and develop a catalog of microseismic events/locations and to determine if there is any relationship to CO2 injection in the field. Identifying the location of any microseismic events will allow for correlation with carbon injection locations and previously characterized geological and structural features such as faults and paleoslopes. Additionally, the borehole array has recorded over 1200 active sources with three sweeps at each source location that were acquired during a nearby 3D VSP. These data were evaluated for their usability and location within an effective radius of the array and were stacked to improve signal-noise ratio and are used to calibrate a full field velocity model to enhance event location accuracy. Funding for this project is provided by the U.S. Department of Energy under Award No. DE-FC26-05NT42591.

  12. Molecular Gas in the Powerful Radio Galaxies 3C~31 and 3C~264 Major or Minor Mergers?

    Lim, J; Combes, F


    We report the detection of $^{12}$CO~($1 \\to 0$) and $^{12}$CO~($2 \\to 1$) emission from the central regions ($\\lesssim 5$--$10 {\\rm kpc}$) of the two powerful radio galaxies 3C~31 and 3C~264. Their individual CO emission exhibits a double-horned line profile that is characteristic of an inclined rotating disk with a central depression at the rising part of its rotation curve. The inferred disk or ring distributions of the molecular gas is consistent with the observed presence of dust disks or rings detected optically in the cores of both galaxies. For a CO to H$_2$ conversion factor similar to that of our Galaxy, the corresponding total mass in molecular hydrogen gas is $(1.3 \\pm 0.2) \\times 10^9 {\\rm M_{\\odot}}$ in 3C~31 and $(0.31 \\pm 0.06) \\times 10^9 {\\rm M_{\\odot}}$ in 3C~264. Despite their relatively large molecular-gas masses and other peculiarities, both 3C~31 and 3C~264, as well as many other powerful radio galaxies in the (revised) 3C catalog, are known to lie within the fundamental plane of normal...

  13. Optical Periodicity Analysis of 3C 446 using Period04

    Fei Guo; Hao Jing Zhang


    All the data of the blazar 3C446 at 8, 4.8, 14 and 22 GHz, presented in publications from 1977 to 2006, have been compiled to generate light curves. The light curves show violent activity of 3C446. Using Period04 analysis method, we have found that there is a period of 7.2 yr, which is consistent with the results that we found using wavelet analysis method. We get the instability region as = 123.83.

  14. Pulsewidth Modulation of Neutral-Point-Clamped Indirect Matrix Converter

    Blaabjerg, Frede; Poh Chiang, Loh; Gao, Feng


    discussed in the existing literature. Addressing the previously described issues, this paper focuses on the operational mode analysis of a three-level indirect matrix converter implemented using a neutral-point-clamped inversion stage and the design of a number of PWM and modulation ratio compensation...... schemes for controlling the converter with improved waveform quality. The performances and practicalities of the designed schemes are verified in simulation and experimentally using an implemented laboratory prototype with some representative results captured and presented in this paper....

  15. Effect of Clamping Rigidity of the Armour on Ballistic Performance

    S. N. Dikshit


    Full Text Available Influence of the manner in which the armour plates are held during their ballistic testing on the armour performance, has been evaluated. One armour plate was clamped rigidly to the test stand while a second plate of identical composition, hardness, and dimensions was hung loosely from the target holder. Both these plates were impacted with the same type of projectiles and over the same impact velocity range. The nature of ballistic damage evaluated indicates that the manner in which the armour is held during ballistic testing has a negligible influence on its performance at least when the mass of the plate is substantially higher than that of the projectile.

  16. Euglycemic clamp insulin sensitivity and longitudinal systolic blood pressure

    Petrie, John R; Malik, Muhammad Omar; Balkau, Beverley;


    and Cardiovascular disease (RISC) study, we measured insulin sensitivity (M/I) using the euglycemic clamp technique in 1073 healthy European adults (587 women, 486 men) aged 30 to 60 years followed up 3 years later. Systolic BP (SBP) at baseline was higher in insulin-resistant women (ie, those in the low sex......Insulin resistance may be an independent risk factor for the development of hypertension, but change in blood pressure (BP) over time has not been adequately studied in healthy individuals fully characterized for insulin sensitivity. In the Relationship between Insulin Sensitivity...

  17. Accelerated commutation for passive clamp isolated boost converters


    An efficient and cost effective bidirectional DC/DC converter reduces switch voltage stress via accelerated commutation allowing use of a low-cost passive clamp circuit in boost mode. The converter includes a primary circuit, transformer and secondary circuit. The primary circuit takes the form of a “full bridge converter,” a “push-pull converter,” or an “L-type converter.”. The primary circuit may include a dissipator such as a snubber circuit or small buck converter. A secondary side of the...

  18. Patch-clamp detection of macromolecular translocation along nuclear pores

    Bustamante J.O.


    Full Text Available The present paper reviews the application of patch-clamp principles to the detection and measurement of macromolecular translocation along the nuclear pores. We demonstrate that the tight-seal 'gigaseal' between the pipette tip and the nuclear membrane is possible in the presence of fully operational nuclear pores. We show that the ability to form a gigaseal in nucleus-attached configurations does not mean that only the activity of channels from the outer membrane of the nuclear envelope can be detected. Instead, we show that, in the presence of fully operational nuclear pores, it is likely that the large-conductance ion channel activity recorded derives from the nuclear pores. We conclude the technical section with the suggestion that the best way to demonstrate that the nuclear pores are responsible for ion channel activity is by showing with fluorescence microscopy the nuclear translocation of ions and small molecules and the exclusion of the same from the cisterna enclosed by the two membranes of the envelope. Since transcription factors and mRNAs, two major groups of nuclear macromolecules, use nuclear pores to enter and exit the nucleus and play essential roles in the control of gene activity and expression, this review should be useful to cell and molecular biologists interested in understanding how patch-clamp can be used to quantitate the translocation of such macromolecules into and out of the nucleus

  19. Phonons in A3C60 Lattice and Structural Dynamics

    Sven Larsson


    Full Text Available The critical temperature ( of superconductivity in A3C60 compounds is generally lower smaller with alkali atoms (A. Furthermore decreases with applied pressure. In the BCS model, these trends are explained by the lower density of states at the Fermi level for a decreased lattice constant (R. There is more than one counterexample, however, suggesting that BCS does not give the whole truth. The most important one is that the compound with the largest lattice constant, Cs3C60, is not superconducting at all at ambient pressure. In this paper we derive a novel model where a negative lattice contribution to Hubbard U, proportional to 1/R, is taken into account. It is possible to explain why A3C60 compounds with A = Li, and Na have a low or are not superconducting at all, and why Cs3C60 is superconducting only at applied pressure and then with the highest of all C60 alkali fullerides. It is concluded that the density of states mechanism derived in the BCS model is in doubt. Nevertheless superconductivity in A3C60 depends on electron-phonon coupling. The dominating phonon is the bond stretching Ag phonon, a breathing phonon for the whole fullerene molecular ion.

  20. Chronic Pancreatitis, Type 3c Diabetes, and Pancreatic Cancer Risk

    David C Whitcomb


    Full Text Available About half of all patients with chronic pancreatitis (CP develop diabetes mellitus (DM due to the loss of islet cell mass, not just beta cells as in Type 1 DM (T1DM, or due to insulin resistance, as in Type 2 DM (T2DM. Patients with DM from loss of islets due to pancreatic disease or resection are diagnosed with pancreatogenic or Type 3c DM (T3cDM. Patients with T3cDM also lose counter-regulatory hormones, such as glucagon and pancreatic polypeptide, and experience maldigestion associated with pancreatic exocrine insufficiency. Patients with T3cDM are therefore more susceptible to hypoglycemia and a mismatch (asynchrony between food ingestion and nutrient absorption. At the same time, the use of incretin therapy is likely useless, since maldigestion leads to the release of higher levels of hind gut hormones, including GLP1. Thus, T3cDM caused by CP or destruction of the islets involves a special class of potential risks and comorbidity that may be overlooked if the CP has not been diagnosed.

  1. New infrared spectral component of the quasar 3C273

    Robson, E.I.; Gear, W.K.; Brown, L.M.J.; Courvoisier, T.J.-L.; Smith, M.G.; Griffin, M.J.; Blecha, A.


    Following the dramatic infrared to millimetre-wavelength flare seen in the quasar 3C273 during 1983, the authors have continued to monitor its overall continuum emission. Recent measurements show that the to 3-mm emission has decayed to a level well below any seen previously, while the emission has remained relatively constant. This behaviour has revealed the presence of an apparently non-variable component which dominates the near-infrared emission in 3C273 and includes the small 'bump' at approx. 3.5 in the power-law continuum.

  2. Imaging Fracking Zones by Microseismic Reverse Time Migration for Downhole Microseismic Monitoring

    Lin, Y.; Zhang, H.


    Hydraulic fracturing is an engineering tool to create fractures in order to better recover oil and gas from low permeability reservoirs. Because microseismic events are generally associated with fracturing development, microseismic monitoring has been used to evaluate the fracking process. Microseismic monitoring generally relies on locating microseismic events to understand the spatial distribution of fractures. For the multi-stage fracturing treatment, fractures created in former stages are strong scatterers in the medium and can induce strong scattering waves on the waveforms for microseismic events induced during later stages. In this study, we propose to take advantage of microseismic scattering waves to image fracking zones by using seismic reverse time migration method. For downhole microseismic monitoring that involves installing a string of seismic sensors in a borehole near the injection well, the observation geometry is actually similar to the VSP (vertical seismic profile) system. For this reason, we adapt the VSP migration method for the common shot gather to the common event gather. Microseismic reverse-time migration method involves solving wave equation both forward and backward in time for each microseismic event. At current stage, the microseismic RTM is based on 2D acoustic wave equation (Zhang and Sun, 2008), solved by the finite-difference method with PML absorbing boundary condition applied to suppress the reflections of artificial boundaries. Additionally, we use local wavefield decomposition instead of cross-correlation imaging condition to suppress the imaging noise. For testing the method, we create a synthetic dataset for a downhole microseismic monitoring system with multiple fracking stages. It shows that microseismic migration using individual event is able to clearly reveal the fracture zone. The shorter distance between fractures and the microseismic event the clearer the migration image is. By summing migration images for many

  3. Diuretic response to acute hypertension is blunted during angiotensin II clamp

    Leong, Patrick K K; Zhang, Yibin; Yang, Li E;


    ("ANG II clamp"). When blood pressure was acutely increased 50-60 mmHg by arterial constriction without ANG II clamp, urine output and endogenous lithium clearance increased 4.0- and 6.7-fold, respectively. With ANG II clamp, the effects of acute hypertension were reduced 50%: urine output...... and endogenous lithium clearance increased two- and threefold, respectively. We conclude that HOE-140, an inhibitor of the B(2) receptor, potentiates the sensitivity of arterial pressure to ANG II and that clamping systemic ANG II levels during acute hypertension blunts the magnitude of the pressure diuretic...

  4. Cryogenic Clamp-on Ultrasonic Flowmeters using Single Crystal Piezoelectric Transducers Project

    National Aeronautics and Space Administration — Clamp-on ultrasound cryogenic flowmeters using single crystal piezoelectric transducers are proposed to enable reliable, accurate cryogenic instrumentation needs in...

  5. Electrically integrated SU-8 clamped graphene drum resonators for strain engineering

    Lee, Sunwoo; Chen, Changyao; Deshpande, Vikram V.; Lee, Gwan-Hyoung; Lee, Ilkyu; Lekas, Michael; Gondarenko, Alexander; Yu, Young-Jun; Shepard, Kenneth; Kim, Philip; Hone, James


    Graphene mechanical resonators are the ultimate two-dimensional nanoelectromechanical systems (NEMS) with applications in sensing and signal processing. While initial devices have shown promising results, an ideal graphene NEMS resonator should be strain engineered, clamped at the edge without trapping gas underneath, and electrically integratable. In this Letter, we demonstrate fabrication and direct electrical measurement of circular SU-8 polymer-clamped chemical vapor deposition graphene drum resonators. The clamping increases device yield and responsivity, while providing a cleaner resonance spectrum from eliminated edge modes. Furthermore, the clamping induces a large strain in the resonator, increasing its resonant frequency.

  6. Mechanism of ATP-driven PCNA clamp loading by S. cerevisiae RFC.

    Chen, Siying; Levin, Mikhail K; Sakato, Miho; Zhou, Yayan; Hingorani, Manju M


    Circular clamps tether polymerases to DNA, serving as essential processivity factors in genome replication, and function in other critical cellular processes as well. Clamp loaders catalyze clamp assembly onto DNA, and the question of how these proteins construct a topological link between a clamp and DNA, especially the mechanism by which ATP is utilized for the task, remains open. Here we describe pre-steady-state analysis of ATP hydrolysis, proliferating cell nuclear antigen (PCNA) clamp opening, and DNA binding by Saccharomyces cerevisiae replication factor C (RFC), and present the first kinetic model of a eukaryotic clamp-loading reaction validated by global data analysis. ATP binding to multiple RFC subunits initiates a slow conformational change in the clamp loader, enabling it to bind and open PCNA and to bind DNA as well. PCNA opening locks RFC into an active state, and the resulting RFC.ATP.PCNA((open)) intermediate is ready for the entry of DNA into the clamp. DNA binding commits RFC to ATP hydrolysis, which is followed by PCNA closure and PCNA.DNA release. This model enables quantitative understanding of the multistep mechanism of a eukaryotic clamp loader and furthermore facilitates comparative analysis of loaders from diverse organisms.

  7. Modeling of SVM Diode Clamping Three-Level Inverter Connected to Grid

    Guo, Yougui; Zeng, Ping; Zhu, Jieqiong


    PLECS is used to model the diode clamping three-level inverter connected to grid and good results are obtained. First the output voltage SVM is described for diode clamping three-level inverter with loads connected to Y. Then the output voltage SVM of diode clamping three-level inverter is simply...... is very powerful tool to real power circuits and it is very easy to simulate them. They have also verified that SVM control strategy is feasible to control the diode clamping three-level inverter....

  8. Data of evolutionary structure change: 1V37A-2YW3C [Confc[Archive

    Full Text Available 1V37A-2YW3C 1V37 2YW3 A C --------MELWLVRH--------GETLWNREGRLLGWT...41 ALA CA 498 2YW3 C 2YW3C...CA 453 2YW3 C 2YW3C.../pdbChain> 2YW3C LEITL-RTEKG EEE - HH...VID> 3 2YW3 C 2YW3C

  9. Trans-Z-source Neutral Point Clamped inverter

    Mo, W.; Loh, P. C.; Li, D.;


    Transformer based Z-source (trans-Z-source) inverters are recently proposed by extending the traditional Z-source inverter with higher buck-boost capability as well as reducing the passive components at the same time. Multi-Level Z-source inverters are single-stage topological solutions used...... modulation scheme, the three-level trans-Z-source inverter can function with minimum of six device commutations per half carrier cycle (same as the traditional buck NPC inverter), while maintaining to produce the designed volt-sec average and inductive voltage boosting at ac output terminals. The designed...... for buck-boost energy conversion with all the favourable advantages of multi-level switching retained. This paper presents three-level trans-Z-source Neutral Point Clamped (NPC) inverter topology, which achieves both the advantages of trans-Z-source and three-level NPC inverter configuration. With proper...

  10. The Double–Double Radio Galaxy 3C293

    S. A. Joshi; S. Nandi; D. J. Saikia; C. H. Ishwara-Chandra; C. Konar


    We present the results of radio continuum observations at frequencies ranging from ∼ 150–5000 MHz of the misaligned double–double radio galaxy (DDRG) 3C293 (J1352+3126) using the GMRT and the VLA, and estimate the time-scale of interruption of jet activity to be less than ∼ 0.1 Myr.

  11. Search for exotic events from the L3+C data

    DING Lin-Kai; HE Zuo-Xiu; HUO An-Xiang; JING Cai-Liu; KUANG Hao-Huai; LEI Yu; LI Li; MA Xin-Hua; MA Yu-Qian; QING Cheng-Rui; WANG Rui-Guang; YAO Zhi-Guo; YU Zhong-Qiang; ZHANG Chao; ZHANG Feng; ZHANG Jing; ZHU Qing-Qi


    An effort to search for Kolar-like events within the data set of the L3+C experiment is reported. From a total of 0.89×1010 triggered events there are no reliable two-prong Kolar-like events observed. The some reasonable assumptions.

  12. Position Measurements of the Core in 3C 66B

    G.-Y. Zhao; Y.-J. Chen; Z.-Q. Shen; H. Sudou; S. Iguchi; Y. Murata; Y. Taniguchi


    It was argued that 3C 66B, a nearby radio galaxy, harbors a supermassive black hole binary (SMBHB). To investigate this, a 4-epoch VLBA phase referencing imaging observation was performed in 2004–2005. Here we present some preliminary results of this project. We found a large position difference compared to previous results.

  13. A decade of 3C technologies: insights into nuclear organization

    de Wit, E.; de Laat, W.


    Over the past 10 years, the development of chromosome conformation capture (3C) technology and the subsequent genomic variants thereof have enabled the analysis of nuclear organization at an unprecedented resolution and throughput. The technology relies on the original and, in hindsight, remarkably

  14. Discovery of a faint optical jet in 3C 120

    Hjorth, J.; Vestergaard, Marianne; Sorensen, A. N.;


    the optical condensation A of the galaxy, which includes the bright 4" radio knot, is found to be 12 % polarized with the electric field vectors perpendicular to the jet. These findings indicate that 3C 120 contains the 6th known extragalactic optical synchrotron jet, quite similar in its properties...

  15. Microwave Radiometer – 3 Channel (MWR3C) Handbook

    Cadeddu, MP


    The microwave radiometer 3-channel (MWR3C) provides time-series measurements of brightness temperatures from three channels centered at 23.834, 30, and 89 GHz. These three channels are sensitive to the presence of liquid water and precipitable water vapor.

  16. Connection Between X-Ray Emission and Relativistic Jets in the Radio Galaxies 3C 111 and 3C 120

    Aller, Margo F.


    This work represents a part of a longterm study of the X-ray flux variability in radio galaxies and its relation to flux and structural changes in the associated radio jet. The work described here included: 1) continued study of the emission properties of the FR I radio galaxy 3C 120 known to exhibit a jet/disk connection from our past work; and 2) the commencement of monitoring of a second radio galaxy, the FR I1 object 3C 111 which was selected because of similar radio and X-ray properties to 3C 120, including the presence of Fe K a emission. The association between X-ray dips and new superluminal components, suggesting a picture in which the radio jet is fed by accretion events near the black hole, was identified in 3C 120 using combined RXTE and radio flux monitoring data and bi-monthly to monthly imaging data from the VLBA at 43 GHz. Such data were also obtained for both targets during the period described here. Specific goals were to more broadly investigate the X-ray dip/superluminal connection in 3C 120, thereby determining the epochs of X-ray minima and superluminal ejections more accurately (and hence more precisely determining the distance between the accretion disk and the core of the radio jet), and to determine whether a similar pattern is present in the data for a second radio galaxy. In 3C 111 a different time scale (longer time delays between X-ray dips and superluminal ejections) was expected due to the higher black hole mass implied by its higher radio luminosity: no black hole mass is published for this object but one can be determined from a PDS analysis of the RXTE data. The addition of the second source to the study would identify whether a similar connection was present in other sources and, if found, would provide important information on how time scale (and hence size scale) of accretion disk/jet systems depends on black hole mass. The grant included funding for the reduction and analysis of data obtained during the time period of Rossi

  17. Surface and downhole shear wave seismic methods for thick soil site investigations

    Hunter, J.A.; Benjumea, B.; Harris, J.B.; Miller, R.D.; Pullan, S.E.; Burns, R.A.; Good, R.L.


    Shear wave velocity-depth information is required for predicting the ground motion response to earthquakes in areas where significant soil cover exists over firm bedrock. Rather than estimating this critical parameter, it can be reliably measured using a suite of surface (non-invasive) and downhole (invasive) seismic methods. Shear wave velocities from surface measurements can be obtained using SH refraction techniques. Array lengths as large as 1000 m and depth of penetration to 250 m have been achieved in some areas. High resolution shear wave reflection techniques utilizing the common midpoint method can delineate the overburden-bedrock surface as well as reflecting boundaries within the overburden. Reflection data can also be used to obtain direct estimates of fundamental site periods from shear wave reflections without the requirement of measuring average shear wave velocity and total thickness of unconsolidated overburden above the bedrock surface. Accurate measurements of vertical shear wave velocities can be obtained using a seismic cone penetrometer in soft sediments, or with a well-locked geophone array in a borehole. Examples from thick soil sites in Canada demonstrate the type of shear wave velocity information that can be obtained with these geophysical techniques, and show how these data can be used to provide a first look at predicted ground motion response for thick soil sites. ?? 2002 Published by Elsevier Science Ltd.

  18. HST Polarimetry of the 3C 273 Jet

    Clautice, Devon; Perlman, Eric S.; Sparks, William B.; Biretta, John A.; O'Dea, Christopher P.; Baum, Stefi Alison; Cheung, Chi C.; Birkinshaw, Mark; Worrall, Diana M.; Martel, Andre; Urry, C. Megan; Stawarz, Lukasz; Coppi, Paolo S.; Uchiyama, Yasunobu; Cara, Mihai; Meisenheimer, Klaus; Begelman, Mitchell C.


    We present preliminary results using HST polarimetry of the jet of 3C 273. Polarization is a critical parameter for understanding jet flows, and has proven essential in characterizing the physics of FR I jets; high-quality HST polarimetry has been done for just two other FR II jets previously. Our recent work on two quasar jets, where we measured high optical polarization in the brightest X-ray knots, has favored the synchrotron emission model over the alternative IC/CMB model for their optical to X-ray emission. These new observations of 3C 273 allow for the determination of the magnetic field structure and confirmation of which emission mechanisms are operating to create its optical to X-ray emission, and will allow us to greatly advance modeling efforts for this jet and nail down its kinetic power, a key unknown parameter for understanding quasars and their cosmological effects.

  19. Effect of clamping pressure on the performance of a PEM fuel cell

    Chang, W.R. [Department of Landscape Architecture, Chung-Hua University, Hsinchu 300 (Taiwan); Hwang, J.J. [Department of Environment and Energy, National University of Tainan, Tainan 700 (Taiwan); Weng, F.B.; Chan, S.H. [Fuel Cell Center and Department of Mechanical Engineering, Yuan Ze University, Nei Li, Taoyuan 320 (Taiwan)


    Examined were the effects of the clamping pressure on the performance of a proton exchange membrane (PEM) fuel cell. The electro-physical properties of the gas diffusion layer (GDL) such as porosity, gas permeability, electrical resistance and thickness were measured using a special-designed test rig under various clamping pressure levels. Correlations for the gas permeability of the GDL were developed in terms of the clamping pressure. In addition, the contact resistance between the GDL and the bipolar (graphite) plate was measured under various clamping pressures. Results showed that at the low clamping pressure levels (e.g. <5 bar) increasing the clamping pressure reduces the interfacial resistance between the bipolar plate and the GDL that enhances the electrochemical performance of a PEM fuel cell. In contrast, at the high clamping pressure levels (e.g. >10 bar), increasing the clamping pressure not only reduces the above Ohmic resistance but also narrows down the diffusion path for mass transfer from gas channels to the catalyst layers. Comprising the above two effects did not promote the power density too much but reduce the mass-transfer limitation for high current density. (author)

  20. Implementing dynamic clamp with synaptic and artificial conductances in mouse retinal ganglion cells.

    Huang, Jin Y; Stiefel, Klaus M; Protti, Dario A


    Ganglion cells are the output neurons of the retina and their activity reflects the integration of multiple synaptic inputs arising from specific neural circuits. Patch clamp techniques, in voltage clamp and current clamp configurations, are commonly used to study the physiological properties of neurons and to characterize their synaptic inputs. Although the application of these techniques is highly informative, they pose various limitations. For example, it is difficult to quantify how the precise interactions of excitatory and inhibitory inputs determine response output. To address this issue, we used a modified current clamp technique, dynamic clamp, also called conductance clamp (1, 2, 3) and examined the impact of excitatory and inhibitory synaptic inputs on neuronal excitability. This technique requires the injection of current into the cell and is dependent on the real-time feedback of its membrane potential at that time. The injected current is calculated from predetermined excitatory and inhibitory synaptic conductances, their reversal potentials and the cell's instantaneous membrane potential. Details on the experimental procedures, patch clamping cells to achieve a whole-cell configuration and employment of the dynamic clamp technique are illustrated in this video article. Here, we show the responses of mouse retinal ganglion cells to various conductance waveforms obtained from physiological experiments in control conditions or in the presence of drugs. Furthermore, we show the use of artificial excitatory and inhibitory conductances generated using alpha functions to investigate the responses of the cells.

  1. Structural insight into β-Clamp and its interaction with DNA Ligase in Helicobacter pylori.

    Pandey, Preeti; Tarique, Khaja Faisal; Mazumder, Mohit; Rehman, Syed Arif Abdul; Kumari, Nilima; Gourinath, Samudrala


    Helicobacter pylori, a gram-negative and microaerophilic bacterium, is the major cause of chronic gastritis, gastric ulcers and gastric cancer. Owing to its central role, DNA replication machinery has emerged as a prime target for the development of antimicrobial drugs. Here, we report 2Å structure of β-clamp from H. pylori (Hpβ-clamp), which is one of the critical components of DNA polymerase III. Despite of similarity in the overall fold of eubacterial β-clamp structures, some distinct features in DNA interacting loops exists that have not been reported previously. The in silico prediction identified the potential binders of β-clamp such as alpha subunit of DNA pol III and DNA ligase with identification of β-clamp binding regions in them and validated by SPR studies. Hpβ-clamp interacts with DNA ligase in micromolar binding affinity. Moreover, we have successfully determined the co-crystal structure of β-clamp with peptide from DNA ligase (not reported earlier in prokaryotes) revealing the region from ligase that interacts with β-clamp.

  2. Infrared spectrophotometry of three Seyfert galaxies and 3C 273

    Cutri, R. M.; Puetter, R. C.; Rudy, R. J.; Willner, S. P.; Aitken, D. K.; Jones, B.; Merrill, K. M.; Roche, P. F.; Russell, R. W.; Soifer, B. T.


    Spectrophotometry in the range 2.1-4.0 microns is presented for the Seyfert galaxies NGC 1068, NGC 4151 and Mrk 231 and the quasar 3C 273, together with broadband and narrowband observations of the Seyfert galaxies in the range 8-13 microns. The spectra of NGC 1068 and NGC 4151 are found to contain a significant component due to starlight, especially at shorter wavelengths. The nonstellar component in NGC 1068 is observed to fall off rapidly at wavelengths shorter than 4 microns, consistent with the interpretation of the excess beyond 5 microns as thermal reradiation by dust. Observations confirm the variability of NGC 4151, and indicate the presence of two components of the flux other than starlight: a nonthermal variable component predominant at shorter wavelengths and a constant, probably thermal component at wavelengths greater than 3 microns. Mrk 231 and 3C 273 exhibit no discernable stellar component and were not observed to vary by more than 10%. Evidence is obtained for a broad minimum in the 8 to 13 micron spectrum of Mrk 231, as well as possible structure between rest wavelengths of 2.8 and 2.9 microns, and the spectrum is not a power law. The spectrum of 3C 273 is consistent with a power law from 1.2 to 10 microns, with small but significant deviations.

  3. The inner kiloparsec of the jet in 3C264

    Lara, L; Cotton, B; Feretti, L; Venturi, T; Lara, Lucas; Giovannini, Gabriele; Cotton, Bill; Feretti, Luigina; Venturi, Tiziana


    We present new multi-frequency EVN, MERLIN and VLA observations of the radio source 3C264, sensitive to linear scales ranging from the parsec to several kiloparsecs. The observations confirm the existence of regions with different properties in the first kiloparsec of the jet. The most remarkable feature is the transition between a well collimated narrow jet at distances from the core below 80 pc, to a conical-shaped wide jet, with an opening angle of 20 degrees. Another change of properties, consisting of an apparent deflection of the jet ridge line and a diminution of the surface brightness, occurs at a distance of 300 pc from the core, coincident with the radius of a ring observed at optical wavelengths. Our observations add new pieces of information on the spectrum of the radio-optical jet of 3C264, with results consistent with a synchrotron emission mechanism and a spectrum break frequency in the infrared. Brightness profiles taken perpendicularly to the jet of 3C264 are consistent with a spine brightene...

  4. The Trails of Superluminal Jet Components in 3C 111

    Kadler, M.; Ros, E.; Perucho, M.; Kovalev, Y. Y.; Homan, D. C.; Agudo, I.; Kellermann, K. I.; Aller, M. F.; Aller, H. D.; Lister, M. L.; Zensus, J. A.


    The parsec-scale radio jet of the broad-line radio galaxy 3C 111 has been monitored since 1995 as part of the 2cm Survey and MOJAVE monitoring observations conducted with the VLBA. Here, we present results from 18 epochs of VLBA observations of 3C 111 and from 18 years of radio flux density monitoring observations conducted at the University of Michigan. A major radio flux-density outburst of 3C 111 occurred in 1996 and was followed by a particularly bright plasma ejection associated with a superluminal jet component. This major event allows us to study a variety of processes associated with outbursts of radio-loud AGN in much greater detail than possible in other cases: the primary perturbation gives rise to the formation of a forward and a backward-shock, which both evolve in characteristically different ways and allow us to draw conclusions about the workflow of jet-production events; the expansion, acceleration and recollimation of the ejected jet plasma in an environment with steep pressure and density gradients are revealed; trailing components are formed in the wake of the primary perturbation as a result of Kelvin- Helmholtz instabilities from the interaction of the jet with the external medium. The jet-medium interaction is further scrutinized by the linear-polarization signature of jet components traveling along the jet and passing a region of steep pressure/density gradients.

  5. Hydrodynamical Simulations of Colliding Jets: Modeling 3C 75

    Molnar, S. M.; Schive, H.-Y.; Birkinshaw, M.; Chiueh, T.; Musoke, G.; Young, A. J.


    Radio observations suggest that 3C 75, located in the dumbbell shaped galaxy NGC 1128 at the center of Abell 400, hosts two colliding jets. Motivated by this source, we perform three-dimensional hydrodynamical simulations using a modified version of the GPU-accelerated Adaptive-MEsh-Refinement hydrodynamical parallel code (GAMER) to study colliding extragalactic jets. We find that colliding jets can be cast into two categories: (1) bouncing jets, in which case the jets bounce off each other keeping their identities, and (2) merging jets, when only one jet emerges from the collision. Under some conditions the interaction causes the jets to break up into oscillating filaments of opposite helicity, with consequences for their downstream stability. When one jet is significantly faster than the other and the impact parameter is small, the jets merge; the faster jet takes over the slower one. In the case of merging jets, the oscillations of the filaments, in projection, may show a feature that resembles a double helix, similar to the radio image of 3C 75. Thus we interpret the morphology of 3C 75 as a consequence of the collision of two jets with distinctly different speeds at a small impact parameter, with the faster jet breaking up into two oscillating filaments.

  6. The deformation of an elastic rod with a clamp sliding along a smooth and curved profile

    Misseroni, D; Zaccaria, D; Bigoni, D


    The design of compliant mechanisms is crucial in several technologies and relies on the availability of solutions for nonlinear structural problems. One of these solutions is given and experimentally validated in the present article for a compliant mechanism moving along a smooth curved profile. In particular, a deformable elastic rod is held by two clamps, one at each end. The first clamp is constrained to slide without friction along a curved profile, while the second clamp moves in a straight line transmitting its motion through the elastic rod to the first clamp. For this system it is shown that the clamp sliding on the profile imposes nontrivial boundary conditions (derived via a variational and an asymptotic approach), which strongly influence buckling and nonlinear structural behaviour. Investigation of this behaviour shows that a compliant mechanism can be designed, which gives an almost neutral response in compression. This behavior could easily be exploited to make a force limiting device. Finally a...

  7. Note: High-efficiency energy harvester using double-clamped piezoelectric beams

    Zheng, Yingmei; Wu, Xuan; Parmar, Mitesh; Lee, Dong-weon, E-mail: [MEMS and Nanotechnology Laboratory, School of Mechanical Systems Engineering, Chonnam National University, Gwangju (Korea, Republic of)


    In this study, an improvement in energy conversion efficiency has been reported, which is realized by using a double-clamped piezoelectric beam, based on uniaxial stretching strain. The buckling mechanism is applied to maximize axial stress in the double-clamped beam. The voltage generated by using the double-clamped piezoelectric beam is higher than that generated by using other conventional structures, such as bending cantilevers coated/sandwiched with piezoelectric film, which is proven both theoretically and experimentally. The power generation efficiency is enhanced by further optimizing the double-clamped structure. The optimized high-efficiency energy harvester utilizing double-clamped piezoelectric beams generates a peak output power of 80 μW, under an acceleration of 0.1g.

  8. Note: high-efficiency energy harvester using double-clamped piezoelectric beams.

    Zheng, Yingmei; Wu, Xuan; Parmar, Mitesh; Lee, Dong-weon


    In this study, an improvement in energy conversion efficiency has been reported, which is realized by using a double-clamped piezoelectric beam, based on uniaxial stretching strain. The buckling mechanism is applied to maximize axial stress in the double-clamped beam. The voltage generated by using the double-clamped piezoelectric beam is higher than that generated by using other conventional structures, such as bending cantilevers coated/sandwiched with piezoelectric film, which is proven both theoretically and experimentally. The power generation efficiency is enhanced by further optimizing the double-clamped structure. The optimized high-efficiency energy harvester utilizing double-clamped piezoelectric beams generates a peak output power of 80 μW, under an acceleration of 0.1g.

  9. On the mechanism of loading the PCNA sliding clamp by RFC.

    Dionne, Isabelle; Brown, Nicola J; Woodgate, Roger; Bell, Stephen D


    Sliding clamps play central roles in a broad range of DNA replication and repair processes. The clamps form circular molecules that must be opened and resealed around DNA by the clamp loader complex to fulfil their function. While most eukaryotes and many archea possess a homo-trimeric PCNA, the PCNA of Sulfolobus solfataricus is a heterotrimer. Here, we exploit the asymmetry of S. solfataricus PCNA to create a series of circularly permuted PCNA subunit fusions, thereby covalently closing defined interfaces within the heterotrimer. Using these concatamers, we investigate the requirements for loading the clamp onto DNA and reveal that a single defined interface within the heterotrimer is opened during the loading process. Subunit-specific interactions between S. solfataricus RFC clamp loader and PCNA permit us to superimpose our data upon the structure of yeast RFC-PCNA complex, thereby presenting a general model for PCNA loading by RFC in archaea and eukaryotes.

  10. A reduction clamp for an aiming component in associated acetabular fractures

    Zhang-Fu Wang


    Full Text Available Background: The treatment of acetabular fractures is complex and requires specialized equipment. However, all currently available instruments have some disadvantages. A new reduction clamp that can firmly enable reduction and not hinder subsequent fixation procedures for some special fracture types is needed. Materials and Methods: In this study, we introduce a new acetabular clamp and its preliminary clinical application in three T-shaped acetabular fractures. Results: This new clamp can successfully pull the posterior column back to the anterior column and firmly maintain the reduction. This clamp′s aiming plate can facilitate the insertion of long lag screws. The clamp is also easy to assemble and use. Conclusion: This reduction clamp is a useful instrument that can facilitate open reduction and internal fixation of acetabular fractures.

  11. Surface-downhole and crosshole geoelectrics for monitoring of brine injection at the Ketzin CO2 storage site

    Rippe, Dennis; Bergmann, Peter; Labitzke, Tim; Wagner, Florian; Schmidt-Hattenberger, Cornelia


    The Ketzin pilot site in Germany is the longest operating on-shore CO2 storage site in Europe. From June 2008 till August 2013, a total of ˜67,000 tonnes of CO2 were safely stored in a saline aquifer at depths of 630 m to 650 m. The storage site has now entered the abandonment phase, and continuation of the multi-disciplinary monitoring as part of the national project "CO2 post-injection monitoring and post-closure phase at the Ketzin pilot site" (COMPLETE) provides the unique chance to participate in the conclusion of the complete life cycle of a CO2 storage site. As part of the continuous evaluation of the functionality and integrity of the CO2 storage in Ketzin, from October 12, 2015 till January 6, 2015 a total of ˜2,900 tonnes of brine were successfully injected into the CO2 reservoir, hereby simulating in time-lapse the natural backflow of brine and the associated displacement of CO2. The main objectives of this brine injection experiment include investigation of how much of the CO2 in the pore space can be displaced by brine and if this displacement of CO2 during the brine injection differs from the displacement of formation fluid during the initial CO2 injection. Geophysical monitoring of the brine injection included continuous geoelectric measurements accompanied by monitoring of pressure and temperature conditions in the injection well and two adjacent observation wells. During the previous CO2 injection, the geoelectrical monitoring concept at the Ketzin pilot site consisted of permanent crosshole measurements and non-permanent large-scale surveys (Kiessling et al., 2010). Time-lapse geoelectrical tomographies derived from the weekly crosshole data at near-wellbore scale complemented by six surface-downhole surveys at a scale of 1.5 km showed a noticeable resistivity signature within the target storage zone, which was attributed to the CO2 plume (Schmidt-Hattenberger et al., 2011) and interpreted in terms of relative CO2 and brine saturations (Bergmann

  12. Simultaneous patch-clamping and calcium imaging in developing dendrites.

    Kleindienst, Thomas; Lohmann, Christian


    Calcium imaging has been used extensively to explore the role of action potential (AP) firing in the development of neuronal structure and synaptic function because increases in intracellular calcium ([Ca(2+)]i) reliably and, within a certain range, linearly reflect neuronal spiking activity. Patterns of APs in individual cells can be deduced from calcium recordings, which have typically been performed at the level of cell bodies. However, neurons are particularly susceptible to phototoxicity when they are illuminated at the soma. Furthermore, for some imaging experiments (e.g., those that address the interactions between dendrites and axons during synapse formation), the cell body of a given neuron may simply not be in the field of view. In these situations, it would be helpful to determine the spiking patterns of a neuron from the calcium activity in its subcellular compartments such as stretches of dendrites or axons. Here, we describe an approach for determining the relationship between AP firing and dendritic calcium transients by simultaneously imaging calcium transients in small dendritic stretches of hippocampal pyramidal neurons in slice cultures from neonatal rats and recording spiking activity with whole-cell patch-clamp recordings in these neurons. These experiments allow us to correlate the electrophysiological spiking pattern with the accompanying changes in the calcium concentration in individual dendritic segments.

  13. Non-Hookean statistical mechanics of clamped graphene ribbons

    Bowick, Mark J.; Košmrlj, Andrej; Nelson, David R.; Sknepnek, Rastko


    Thermally fluctuating sheets and ribbons provide an intriguing forum in which to investigate strong violations of Hooke's Law: Large distance elastic parameters are in fact not constant but instead depend on the macroscopic dimensions. Inspired by recent experiments on free-standing graphene cantilevers, we combine the statistical mechanics of thin elastic plates and large-scale numerical simulations to investigate the thermal renormalization of the bending rigidity of graphene ribbons clamped at one end. For ribbons of dimensions W ×L (with L ≥W ), the macroscopic bending rigidity κR determined from cantilever deformations is independent of the width when W expected. When W >ℓth , however, this thermally renormalized bending rigidity begins to systematically increase, in agreement with the scaling theory, although in our simulations we were not quite able to reach the system sizes necessary to determine the fully developed power law dependence on W . When the ribbon length L >ℓp , where ℓp is the W -dependent thermally renormalized ribbon persistence length, we observe a scaling collapse and the beginnings of large scale random walk behavior.

  14. Multiwavelength and Polarimetric Analysis of the Flat Spectrum Radio Quasars 3C 273 and 3C 279

    Fernandes, Sunil; Patiño-Álvarez, Victor; Chavushyan, Vahram; Schlegel, Eric M.; Lopez-Rodriguez, Enrique; León-Tavares, Jonathan; Carrasco, Luis; Valdés, José; Carramiñana, Alberto


    This poster presents results of multiwavelength analyses of 3C 273 and 3C 279. The main goals were to identify the gamma-ray emission region and dominant high-energy emission processes. Our methodology consisted of analyzing light curves from radio to gamma-rays over 6 - 8 years and polarimetric, spectral and line emission behavior.In 3C 279, we found that the emission from millimeter to ultraviolet was simultaneous and therefore co-spatial. We identified two active states where different high-energy emission processes were dominant. We found multiwavelength flaring events consistent with component ejections and shocks. We proposed that the gamma-ray emission region changed over time based on observations of both simultaneous and delayed gamma-rays emission with respect to low-energy emission during different time-frames.In 3C 273, we identified a non-thermal flare related to a component ejection and a thermal flare related to accretion. From reverberation mapping we found that the broad line region dynamical behavior over time possibly affects the derived supermassive black hole mass.In both objects we found that the gamma-ray spectral index was variable, and a trend of harder spectral index with higher gamma-ray luminosity. From the identification of different dominant high-energy emission processes, we concluded that the dominant high-energy emission mechanism changes with time. Overall, we concluded that similar results from both objects points to behavior that is potentially common to flat spectrum radio quasars. Increasing the sample size of objects analyzed with similar methodologies will provide more results to confirm or refine our conclusions.


    Martin L. Gildea; Stephen P. Farrington, P.E.; Wesley L. Bratton, Ph.D., PE


    Conventional soil and groundwater sampling procedures present many opportunities for loss of volatile organic compounds (VOC) by exposing sample media to the atmosphere during transfers between sampling devices and containers, ultimately affecting the quality of the analytical results. Inaccurate characterization data often leads to improperly designed remedial systems that slow the clean-up process and increase the cost. For these reasons, in situ methods for sample extraction and real time analysis provide attractive alternatives to conventional sampling and analysis. Under funding from the U.S. Department of Energy's Federal Energy Technology Center (FETC), the investigators continued development of a system that combines High Speed Gas Chromatography techniques with Cone Penetration Testing to achieve near-real time analysis of soils and groundwater for chlorinated and aromatic hydrocarbon contamination (PCE, TCE, BTEX) during site characterization. The system combines three new CPT tools with an up-hole analyzer. The Dynamic Thermal Desorption (DTD) probe provides the up-hole analyzer with continuously sampled soil gas, enhancing detection limits by heating the soil matrix during penetration to thermally desorb volatile organic contaminants. The CPT In Situ Purge Probe (CISP) purges a water sample in situ, transferring the purge gas up-hole for analysis. Alternatively, soil gas or purge gas from the DTD probe or the CISP can be diverted to a Downhole Trap Module (DTM), which traps contaminants on conventional trap media for later analysis in a fixed laboratory. While the effectiveness of the CISP was hampered by the tendency of fine grained materials to clog its sample inlet filter, the DTD probe and the DTM were demonstrated to perform their intended functions well.

  16. Lessons Learned From the Analysis of the SAFOD Downhole Instrument Package.

    Johnson, Wade; Mencin, David; Mattioli, Glen


    In September of 2008 a downhole instrument package (DIP) consisting of a string of seismometers and tilt meters in isolated pressure vessels (PODs) was installed in the SAFOD main borehole. This package was designed to protect the sensors from the corrosive borehole environment and to operate for two years. The SAFOD borehole is not sealed at the bottom allowing borehole gasses and fluids infiltratration. Previous short-term installations of instruments in the SAFOD main borehole had also failed as a result of corrosion of the wireline cable head. The average failure time for these installations was two weeks. The use of stainless steel tubing connected to the pressure vessels through gas tight fittings was designed to block borehole fluid and gas infiltration of the individual instruments within the PODs. Unfortunately, the DIP completely failed within a month of its installation. In October of 2010, the DIP was removed from the borehole and a failure analysis was performed. This analysis involved to following steps: 1. Analysis of data to understand timeline of failure 2. Remove instrument safely, maintaining integrity of spliced section and documenting any external clues. Test instrument at surface 3. Open PODs in a way that allows for sampling and avoids damaging instruments. 4. Chemical analysis of fluids recovered from splices and PODs. 5. Instrument failure analysis by the instrument manufacturers. The analysis found that there were several design flaws in the DIP. This included the use of motor oil to take up air space in the individual PODs, use of a large number of gas tight seals, lack of internal seals, poorly done solder joints, use of non-temperature rated sensors, and lack of management oversight. The lessons learned from the attempts to instrument the SAFOD borehole are critical to the success of future deep borehole projects.

  17. 随钻井下姿态测量系统%Attitude measurement system Downhole drilling

    贾衡天; 张程光; 高文凯; 管康; 范锦辉; 彭浩; 邓乐


    In drilling oil drilling it′s important to accurately understand the attitude information of drilling tool. By measuring the attitude information accurately it can grasp the orientation parameters of borehole.With the development of petroleum industry in recent years, it need to use precise trajectory control technology for oil drilling technology to adapt to directional wells, horizontal wells and large displacement well application.This paper designs a downhole attitude measurement system which can work in drilling conditions. By measuring the signal of three axis gyroscope, accelerometer and magnetic sensor. and doing corresponding algorithm, it can get the attitude information of underground drilling.%在随钻石油钻探时需要准确地了解钻具的姿态信息,通过对姿态信息的测量可以准确地掌握井眼的方位参数。随着近年来石油工业的发展,要求石油钻井技术采用精确的轨迹控制技术,适应定向井、水平井和大位移井等应用的需要。设计一种井下姿态测量系统,可以工作在随钻条件下,通过测量三轴陀螺仪、加速度计和磁阻传感器的信号并进行相应的算法处理,得到钻具在井下的姿态信息。

  18. Structure and Properties of the Nonface-Spiral Fullerenes T-C380, D3-C384, D3-C440, and D3-C672 and Their Halma and Leapfrog Transforms

    Wirz, Lukas; Tonner, Ralf; Avery, James Emil;


    The structure and properties of the three smallest nonface-spiral (NS) fullerenes NS-T-C380, NS-D3-C384, NS-D3-C440, and the first isolated pentagon NS-fullerene, NS-D3-C672, are investigated in detail. They are constructed by either a generalized face-spiral algorithm or by vertex insertions fol...

  19. Experience with Server Self Service Center (S3C)

    Sucik, J; CERN. Geneva. IT Department


    CERN has a successful experience with running Server Self Service Center (S3C) for virtual server provisioning which is based on Microsoft® Virtual Server 2005. With the introduction of Windows Server 2008 and its built-in hypervisor based virtualization (Hyper-V) there are new possibilities for the expansion of the current service. This paper describes the architecture of the redesigned virtual Server Self Service based on Hyper-V which provides dynamically scalable virtualized resources on demand as needed and outlines the possible implications on the future use of virtual machines at CERN.

  20. Delta-function Approximation SSC Model in 3C 273

    S. J. Kang; Y. G. Zheng; Q. Wu


    We obtain an approximate analytical solution using approximate calculation on the traditional one-zone synchrotron self-Compton (SSC) model. In this model, we describe the electron energy distribution by a broken power-law function with a sharp cut-off, and non-thermal photons are produced by both synchrotron and inverse Compton scattering of synchrotron photons. We calculate the radiation energy spectrum of electrons by the function. We apply this model to the multi-wavelength Spectral Energy Distributions (SED) of the 3C 273 in different states, and obtain excellent fits to the observed spectra of this source.

  1. Experience with Server Self Service Center (S3C)

    Sucik, J


    CERN has a successful experience with running Server Self Service Center (S3C) for virtual server provisioning which is based on Microsoft® Virtual Server 2005. With the introduction of Windows Server 2008 and its built-in hypervisor based virtualization (Hyper-V) there are new possibilities for the expansion of the current service. This paper describes the architecture of the redesigned virtual Server Self Service based on Hyper-V which provides dynamically scalable virtualized resources on demand as needed and outlines the possible implications on the future use of virtual machines at CERN.

  2. Study of Structural Parameters of the Inlet of Downhole Hydrocyclones%井下水力旋流器入口结构参数试验研究

    吕凤霞; 张淑艳; 胡春伟


    Three different inlets of hydrocyclone are studied in combination with the construction of a downhole system and hydrocyclone. By comparing the relationship between the inlet structure & dimensional parameter of hydrocyclone and separation efficiency & pressure loss, the highest efficiency is obtained from the inlet of an involute curve with increasing depth-width ratio from the three types, in which the separation efficiency and pressure loss all drops slowly,for the length of the channel decreases, while it drops rapidly in the other two. The flow guiding ability of the inlet affects the separation efficiency greatly, so the corresponding involute type of inlet of hydrocyclone fits for downhole oil-water separation is optimized, which serves as a basis for the structural design of downhole hydrocyclone.

  3. The high energy spectrum of 3C 273

    Esposito, V; Jean, P; Tramacere, A; Türler, M; Lähteenmäki, A; Tornikoski, M


    Aims. The high energy spectrum of 3C 273 is usually understood in terms of inverse-Compton emission in a relativistic leptonic jet. This model predicts variability patterns and delays that could be tested with simultaneous observations from the radio to the GeV range. Methods. The instruments IBIS, SPI, JEM-X on board INTEGRAL, PCA on board RXTE, and LAT on board Fermi have enough sensitivity to follow the spectral variability of 3C 273 from the keV to the GeV. We looked for correlations between the different energy bands, including radio data at 37 GHz collected at the Mets\\"ahovi Radio Observatory and built quasi-simultaneous multiwavelength spectra in the high energy domain when the source is flaring either in the X-rays or in the {\\gamma} rays. Results. Both temporal and spectral analysis suggest a two-component model to explain the complete high energy spectrum. X-ray emission is likely dominated by a Seyfert-like component while the {\\gamma}-ray emission is dominated by a blazar-like component produced ...

  4. Far-infrared polarisation of the quasar 3C 279

    Klaas, U; Clavel, J; Klaas, Ulrich; Laureijs, Rene J.; Clavel, Jean


    We present the first FIR polarisation results of the OVV quasar 3C 279 obtained with ISOPHOT for two epochs in 1996 and 1997. We describe its integral polarisation properties at a wavelength of 170 micron where the source shows a maximum in its energy distribution. After a gamma-ray flare in January 1996, a polarisation of 23 % closely aligned with the radio jet axis was measured in July 1996. In June 1997, the polarisation degree had decreased to 6.5 % with a less good alignment. On the other hand, the total 170 micron flux is the same for both epochs. Our measurements provide additional constraints for the multi-wavelength properties of synchrotron emission in radio jets and the temporal evolution of these properties: they show that the FIR radiation of 3C 279 is optically thin and that its origin is very close to the core. The variability of the FIR polarisation without any change of the total FIR flux can be explained by a disordering of the magnetic field in between the core and the first stationary VLBI...

  5. XMM-Newton observations of 3C 273

    Page, K L; Done, C; O'Brien, P T; Reeves, J N; Sembay, S; Stuhlinger, M


    A series of nine XMM-Newton observations of the radio-loud quasar 3C 273 are presented, concentrating mainly on the soft excess. Although most of the individual observations do not show evidence for iron emission, co-adding them reveals a weak, broad line (EW ~ 56 eV). The soft excess component is found to vary, confirming previous work, and can be well fitted with multiple blackbody components, with temperatures ranging between ~40 and ~330 eV, together with a power-law. Alternatively, a Comptonisation model also provides a good fit, with a mean electron temperature of ~350 eV, although this value is higher when the soft excess is more luminous over the 0.5-10 keV energy band. In the RGS spectrum of 3C 273, a strong detection of the OVII He-alpha absorption line at zero redshift is made; this may originate in warm gas in the local intergalactic medium, consistent with the findings of both Fang et al. (2003) and Rasmussen et al. (2003).

  6. Clusters of galaxies associated with quasars. I. 3C 206

    Ellingson, E.; Yee, H.K.C.; Green, R.F.; Kinman, T.D. (Steward Observatory, Tucson, AZ (USA); Montreal Universite (Canada); Kitt Peak National Observatory, Tucson, AZ (USA))


    Multislit spectroscopy and three-color CCD photometry of the galaxies in the cluster associated with the quasar 3C 206 (PKS 0837-12) at z = 0.198 are presented. This cluster is the richest environment of any low-redshift quasar observed in an Abell richness class 1 cluster. The cluster has a very flattened structure and a very concentrated core about the quasar. Most of the galaxies in this field have colors and luminosities consistent with normal galaxies at this redshift. The background-corrected blue fraction of galaxies is consistent with values for other rich clusters. The existence of several blue galaxies in the concentrated cluster core is an anomaly for a region of such high galaxy density, however, suggesting the absence of a substantial intracluster medium. This claim is supported by the Fanaroff-Riley (1974) class II morphology of the radio source. The velocity dispersion calculated from 11 spectroscopically confirmed cluster members is 500 + or - 110 km/s, which is slightly lower than the average for Abell class 1 clusters. A high frequency of interaction between the quasar host galaxy and cluster core members at low relative velocities, and a low intracluster gas pressure, may comprise a favorable environment for quasar activity. The properties of the cluster of galaxies associated with 3C 206 are consistent with this model. 59 refs.

  7. The Trails of Superluminal Jet Components in 3C111

    Kadler, M; Perucho, M; Kovalev, Y Y; Homan, D C; Agudo, I; Kellermann, K I; Aller, M F; Aller, H D; Lister, M L; Zensus, J A


    In 1996, a major radio flux-density outburst occured in the broad-line radio galaxy 3C111. It was followed by a particularly bright plasma ejection associated with a superluminal jet component, which has shaped the parsec-scale structure of 3C111 for almost a decade. Here, we present results from 18 epochs of Very Long Baseline Array (VLBA) observations conducted since 1995 as part of the VLBA 2 cm Survey and MOJAVE monitoring programs. This major event allows us to study a variety of processes associated with outbursts of radio-loud AGN in much greater detail than has been possible in other cases: the primary perturbation gives rise to the formation of a leading and a following component, which are interpreted as a forward and a backward-shock. Both components evolve in characteristically different ways and allow us to draw conclusions about the work flow of jet-production events; the expansion, acceleration and recollimation of the ejected jet plasma in an environment with steep pressure and density gradien...

  8. Photoluminescence Properties of Nanocrystalline 3C-SiC Films

    YU Wei; LU Xue-qin; LU Wan-bing; HAN Li; FU Guang-sheng


    Nanocrystalline (nc) 3C-SiC films on the Si substrate were prepared by the helicon wave plasma enhanced chemical vapor deposition (HW-PECVD) technique. With the SiH4-CH4 gas flow ratio changing, the films exhibit different photoluminescence (PL) characteristics. Under the stoichiometric condition, the PL peak redshift from 470 nm to 515 nm is detected with the increase of excitation wavelength, which can be attributed to the quantum confinement effect radiation of 3C-SiC nanocrystals of different sizes. However, the appearance of an additional PL band at 436 nm in Si-rich film might be sourced back to the excess of Si defect centers in it. This is also the case for C-rich film for its PL band lying at 570 nm. The results above quoted indicate an important influence of gas flow ratio on the PL properties of the SiC films providing an effective guidance for analyzing the luminescence mechanism and exploring the high-efficiency light emission of the SiC films.

  9. Modelling the $\\gamma$-ray variability of 3C 273

    Zheng, Y G; Huang, B R; Kang, S J


    We investigate MeV-GeV $\\gamma$-ray outbursts in 3C 273 in the frame of a time-dependent one-zone synchrotron self-Compton (SSC) model. In this model, electrons are accelerated to extra-relativistic energy through the stochastic particle acceleration and evolve with the time, nonthermal photons are produced by both synchrotron and inverse Compton scattering of synchrotron photons. Moreover, nonthermal photons during a quiescent are produced by the relativistic electrons in the steady state and those during a outburst are produced by the electrons whose injection rate is changed at some time interval. We apply the model to two exceptionally luminous $\\gamma$-ray outbursts observed by the Fermi-LAT from 3C 273 in September, 2009 and obtain the multi-wavelength spectra during the quiescent and during the outburst states, respectively. Our results show that the time-dependent properties of outbursts can be reproduced by adopting the appropriate injection rate function of the electron population.

  10. Analysis of the role of PCNA-DNA contacts during clamp loading

    Goedken Eric R


    Full Text Available Abstract Background Sliding clamps, such as Proliferating Cell Nuclear Antigen (PCNA in eukaryotes, are ring-shaped protein complexes that encircle DNA and enable highly processive DNA replication by serving as docking sites for DNA polymerases. In an ATP-dependent reaction, clamp loader complexes, such as the Replication Factor-C (RFC complex in eukaryotes, open the clamp and load it around primer-template DNA. Results We built a model of RFC bound to PCNA and DNA based on existing crystal structures of clamp loaders. This model suggests that DNA would enter the clamp at an angle during clamp loading, thereby interacting with positively charged residues in the center of PCNA. We show that simultaneous mutation of Lys 20, Lys 77, Arg 80, and Arg 149, which interact with DNA in the RFC-PCNA-DNA model, compromises the ability of yeast PCNA to stimulate the DNA-dependent ATPase activity of RFC when the DNA is long enough to extend through the clamp. Fluorescence anisotropy binding experiments show that the inability of the mutant clamp proteins to stimulate RFC ATPase activity is likely caused by reduction in the affinity of the RFC-PCNA complex for DNA. We obtained several crystal forms of yeast PCNA-DNA complexes, measuring X-ray diffraction data to 3.0 Å resolution for one such complex. The resulting electron density maps show that DNA is bound in a tilted orientation relative to PCNA, but makes different contacts than those implicated in clamp loading. Because of apparent partial disorder in the DNA, we restricted refinement of the DNA to a rigid body model. This result contrasts with previous analysis of a bacterial clamp bound to DNA, where the DNA was well resolved. Conclusion Mutational analysis of PCNA suggests that positively charged residues in the center of the clamp create a binding surface that makes contact with DNA. Disruption of this positive surface, which had not previously been implicated in clamp loading function, reduces RFC

  11. A Central Swivel Point in the RFC Clamp Loader Controls PCNA Opening and Loading on DNA

    Sakato, Miho; O’Donnell, Mike; Hingorani, Manju M.


    Replication Factor C (RFC) is a 5-subunit complex that loads PCNA clamps onto primer-template DNA (ptDNA) during replication. RFC subunits belong to the AAA+ superfamily, and their ATPase activity drives interactions between the clamp loader, clamp, and ptDNA, leading to topologically linked PCNA•ptDNA. We report the kinetics of transient events in S. cerevisiae RFC catalyzed PCNA loading, including ATP-induced RFC activation, PCNA opening, ptDNA binding, ATP hydrolysis, PCNA closing and PCNA...

  12. Modelling the clamping force distribution among chips in press-pack IGBTs using the finite element method

    Hasmasan, Adrian Augustin; Busca, Christian; Teodorescu, Remus


    In this paper, a FEM (finite element method) based mechanical model for PP (press-pack) IGBTs (insulated gate bipolar transistors) is presented, which can be used to calculate the clamping force distribution among chips under various clamping conditions. The clamping force is an important paramet...

  13. Influence of axial deformation of pipe string on down-hole operation%管柱轴向变形对井下作业的影响



    This paper reviews the impact of axial deformation of string on different down-hole operating conditions along with the importance of the calculation on axial deformation for the establishment of calculation model of down-hole string axial deformation theory.%综合分析了管柱轴向变形对井下不同作业工况的影响,阐述了计算井下作业管柱轴向变形的重要性,为建立井下作业管柱轴向变形理论计算模型做了很好的铺垫。

  14. An optical inverse-Compton hotspot in 3C196?

    Hardcastle, M J


    Several hotspots of FRII radio sources have previously been detected in the X-ray at a flux level consistent with the X-rays being due to inverse-Compton scattering of radio synchrotron photons (`synchrotron self-Compton'), if the magnetic fields in the hotspots are close to their equipartition values. However, the number of hotspots compact and bright enough to exhibit detectable X-ray emission is small, so it is worth searching for synchrotron self-Compton emission in the optical, in spite of the obvious observational difficulties of such an approach. In this note I report on a possible detection of an optical inverse-Compton hotspot in a deep Hubble Space Telescope observation of the distant quasar 3C196, at a level which implies a hotspot magnetic field strength close to equipartition if the electrons have a low-energy cutoff around gamma ~ 500.

  15. An optical inverse-Compton hotspot in 3C 196?

    Hardcastle, M. J.


    Several hotspots of FRII radio sources have previously been detected in the X-ray at a flux level consistent with the X-rays being due to inverse-Compton scattering of radio ``synchrotron photons synchrotron self-Compton'', if the magnetic fields in the hotspots are close to their equipartition values. However, the number of hotspots compact and bright enough to exhibit detectable X-ray emission is small, so it is worth searching for synchrotron self-Compton emission in the optical, in spite of the obvious observational difficulties of such an approach. In this note I report on a possible detection of an optical inverse-Compton hotspot in a deep Hubble Space Telescope observation of the distant quasar 3C 196, at a level which implies a hotspot magnetic field strength close to equipartition if the electrons have a low-energy cutoff around gamma ~ 500.

  16. Multifrequency observations of the superluminal quasar 3C 345

    Bregman, J. N.; Glassgold, A. E.; Huggins, P. J.; Neugebauer, G.; Soifer, B. T.; Matthews, K.; Roellig, T. P. L.; Bregman, J. D.; Witteborn, F. C.; Lester, D. F.


    Attention is given to the continuum properties of the superluminal quasar 3C 345, on the basis of radio, optical, IR, and X-ray frequency monitorings, as well as by means of simultaneous multifrequency spectra extending from the radio through the X-ray bands. Radio outbursts, which appear to follow IR-optical outbursts by about one year, first occur at the highest frequencies, as expected from optical depth effects; the peak flux is nevertheless often reached at several frequencies at once. The beginning of outbursts, as defined by mm-measurements, corresponds to the appearance of the three known 'superluminal' components. An increase in the X-ray flux during 1979-1980 corresponds to increased radio flux, while the IR flux changes in the opposite sense.

  17. The Multifrequency Campaign on 3C 279 in January 2006

    Collmar, W; Krichbaum, T P; Agudo, I; Bottacini, E; Bremer, M; Burwitz, V; Cuccchiara, A; Grupe, D; Gurwell, M


    We present the results of a multifrequency campaign from radio to hard X-ray energies on the blazar 3C 279 during an optical high-state in January 2006. We give the observational results (multifrequency light curves and spectra) and compile an SED. This complements an SED from an optical low-state in June 2003. Surprisingly the two SEDs differ only in their high-energy synchrotron emission (near-IR - UV), while the low-energy inverse-Compton emission (X- to Gamma-rays) remained unchanged. By interpreting with a steady-state leptonic emission model, the variability among the SED can be reproduced by a change solely of the low-energy cutoff of the relativistic electron distribution. In an internal shock model for blazar emission, such a change could e.g. achieved through a varying relative Lorentz factor of colliding shells producing internal shocks in the jet.

  18. Downhole Logging Measurements from IODP Expedition 313: an Overview and Future Directions

    Inwood, J.; Lofi, J.; Scienceparty, E.


    Downhole geophysical measurements and images allow the characterization of the lithology and physical properties of the subsurface, thus making a powerful contribution to the evaluation of facies and sediment composition and to the recognition of key surfaces in siliciclastic successions. The mission-specific IODP Expedition 313 (May-July 2009) cored and logged sequences deposited on the New Jersey continental margin during the post-Eocene ice-house world. One of the aims of the Expedition is to evaluate sequence stratigraphic facies models that predict depositional environments, sediment compositions, and stratal geometries in response to sea-level change. 5800 m of wireline logging data were collected in three boreholes drilled to a composite total of 2062 m; here we present an overview of these data. Continuous through-pipe spectral gamma ray logs were acquired in each borehole and repeated in open hole intervals for calibration. Magnetic susceptibility, resistivity, sonic and acoustic image logs were obtained in open hole at key intervals and/or where borehole conditions allowed. Due to space limitations on the offshore platform, Exp313 cores were not split. Samples from core catchers, whole-round multi-sensor track measurements, vertical seismic profiles and these logging data comprise the full data set at the time of this writing. Preliminary analysis shows lateral and vertical changes in the physical properties of the sediments that enable us to distinguish depositional intervals at several scales (cms - tens of meters). Medium to high resolution acoustic images of the borehole walls reveal sedimentary characteristics even within intervals of low core recovery, and provide an accurate core-depth positioning of some key surfaces. Likewise, the continuous spectral gamma logs allow assessment of lithologies in intervals with incomplete core recovery, and show great promise of providing excellent log-core-seismic correlations once the cores are split and fully

  19. Lithological controls on gas hydrate saturation: Insights from signal classification of NMR downhole data

    Bauer, Klaus; Kulenkampff, Johannes; Henninges, Jan; Spangenberg, Erik


    Nuclear magnetic resonance (NMR) downhole data are analyzed with a new strategy to study gas hydrate-bearing sediments in the Mackenzie Delta (NW Canada). NMR logging is a powerful tool to study geological reservoir formations. The measurements are based on interactions between the magnetic moments of protons in geological formation water and an external magnetic field. Inversion of the measured raw data provides so-called transverse relaxation time (T2) distribution curves or spectra. Different parts of the T2 curve are related with distinct pore radii and corresponding fluid components. A common practice in the analysis of T2 distribution curves is to extract single-valued parameters such as apparent total porosity. Moreover, the derived total NMR apparent porosity and the gamma-gamma density log apparent porosity can be combined to estimate gas hydrate saturation in hydrate-bearing sediments. To avoid potential loss of information, in our new approach we analyze the entire T2 distribution curves as quasi-continuous signals to characterize the rock formation. The approach is applied to NMR data measured in gas hydrate research well Mallik 5L-38. We use self-organizing maps, a neural network clustering technique, to subdivide the data set of NMR T2 distribution curves into classes with a similar and distinctive signal shape. The method includes (1) preparation of data vectors, (2) unsupervised learning, (3) cluster definition, and (4) classification and depth mapping of all NMR signals. Each signal class thus represents a specific pore size distribution which can be interpreted in terms of distinct lithologies and reservoir types. A key step in the interpretation strategy is to reconcile the NMR classes with other log data not considered in the clustering analysis, such as gamma ray, photo-electric factor, hydrate saturation, and other logs. Our results defined six main lithologies within the target zone. Gas hydrate layers were recognized by their low signal

  20. Anisotropies in the HI gas distribution toward 3C 196

    Kalberla, P. M. W.; Kerp, J.


    Context. The local Galactic Hi gas was found to contain cold neutral medium (CNM) filaments that are aligned with polarized dust emission. These filaments appear to be dominated by the magnetic field and in this case turbulence is expected to show distinct anisotropies. Aims: We use the Galactic Effelsberg-Bonn Hi Survey (EBHIS) to derive 2D turbulence spectra for the Hi distribution in direction to 3C 196 and two more comparison fields. Methods: Prior to Fourier transform we apply a rotational symmetric 50% Tukey window to apodize the data. We derive average as well as position angle dependent power spectra. Anisotropies in the power distribution are defined as the ratio of the spectral power in orthogonal directions. Results: We find strong anisotropies. For a narrow range in position angle, in direction perpendicular to the filaments and the magnetic field, the spectral power is on average more than an order of magnitude larger than parallel. In the most extreme case the anisotropy reaches locally a factor of 130. Anisotropies increase on average with spatial frequency as predicted by Goldreich & Sridhar (1995, ApJ, 438, 763), at the same time the Kolmogorov spectral index remains almost unchanged. The strongest anisotropies are observable for a narrow range in velocity and decay with a power law index close to -8/3, almost identical to the average isotropic spectral index of -2.9 <γ< -2.6. Conclusions: Hi filaments, associated with linear polarization structures in LOFAR observations in direction to 3C 196, show turbulence spectra with marked anisotropies. Decaying anisotropies appear to indicate that we witness an ongoing shock passing the Hi and affecting the observed Faraday depth.

  1. HT/HP x-tree and downhole fiber optic connectors and their use on subsea intelligent wells

    Wright, Perry; Barlow, Stewart


    Offshore Oil and Gas R&D has been committed to improved reservoir performance through production monitoring. Technology improvements in these areas offer the greatest potential returns through increased knowledge of the reservoir, and the improvements in real-time production control that the technology and knowledge base can provide. One area of technology that supports this development is the growing application of fiber optic sensors for reservoir and production monitoring. These sensors cannot function in isolation, and need support in the form of fiber optic connection systems for x-tree penetrations. ODI have been developing products for fiber optic tree penetrations and down-hole wet connections for the last 4 years, working with Intelligent Wells Group at BP America Production Company in Houston. This paper discusses the application and reliability of fiber optic connectors, and reviews the development of the ODI I-CONN connection system and its application for vertical and horizontal x-trees, work-over systems and running tools, and down-hole systems.

  2. Observations Using the Taipei Basin Broadband Downhole Seismic Network: The 26 December 2006, Pingtung Earthquake Doublet, Taiwan

    Win-Gee Huang


    Full Text Available To monitor the fault activity in the Taipei area, a new broadband downhole seismic network comprised of three stations was established in the Taipei Basin over a period of three years, 2005 - 2007. The network geometry is a triangle with a station spacing of about 12 km covering the entire Taipei Basin. Each station has two holes of different depths containing modern instruments, including a low-gain broadband seismometer. The largest depth is 150 m. We report our first experience on the installation and operation of the broadband downhole seismic network in the Taipei Basin. Some representative records from the Pingtung earthquake doublet in December 2006 are shown here. Ground displacement during the Pingtung earthquake doublet can be recovered from the velocity records without the baseline corrections that are required for the acceleration records. Our network offers excellent data for accurate and effective characterization of seismic motion in the study area. Seismic data from this network will be shared with other research institutions in Taiwan and abroad for further research.

  3. Application of multi-stage, multi-disk type downhole seismic source; Tadanshiki taso enbangata koseinai shingen no tekiyosei

    Shimada, N. [Japan National Oil Corp., Tokyo (Japan); Shoji, Y. [Oyo Corp., Tokyo (Japan)


    A multi-stage, multi-disk type seismic source was developed as a downhole seismic source. The seismic source is an improved version of the downhole seismic source of a system in which an elastic wave is generated by a weight accelerated by restitutive force of a spring striking the upper part of a laminated structure consisted of metal disks and elastic bodies installed in water in a well. Enhancing the vibration exciting efficiency requires impedance radiated from the disks to be increased. The multi-disk structure was adopted because of restrictions on the disk area under the limiting condition of being inside the well. Further limitation has still existed, which led to finally structuring the multi-disk type to a multi-stage construction to increase the radiated impedance. In order to increase average velocity on the radiation surface, mass relationship between the hammer and the anvil was sought so that the maximum velocity is achieved at the process of converting motion energies among the hammer, anvil and disks. The anvil mass may sufficiently be 50% to 100% of the hammer mass. The equipment was installed in an actual oil well for testing. This seismic source was verified to have sufficient applicability in the cross hole measurement. 5 refs., 7 figs., 1 tab.

  4. Implementation of a fast 16-Bit dynamic clamp using LabVIEW-RT.

    Kullmann, Paul H M; Wheeler, Diek W; Beacom, Joshua; Horn, John P


    The dynamic-clamp method provides a powerful electrophysiological tool for creating virtual ionic conductances in living cells and studying their influence on membrane potential. Here we describe G-clamp, a new way to implement a dynamic clamp using the real-time version of the Lab-VIEW programming environment together with a Windows host, an embedded microprocessor that runs a real-time operating system and a multifunction data-acquisition board. The software includes descriptions of a fast voltage-dependent sodium conductance, delayed rectifier, M-type and A-type potassium conductances, and a leak conductance. The system can also read synaptic conductance waveforms from preassembled data files. These virtual conductances can be reliably implemented at speeds LabVIEW should make it relatively easy for others to adapt G-clamp for new experimental applications.

  5. Suppression of Spiral Waves by Voltage Clamp Techniques in a Conductance-Based Cardiac Tissue Model

    YU Lian-Chun; MA Jun; ZHANG Guo-Yong; CHEN Yong


    A new control method is proposed to control the spatio-temporal dynamics in excitable media, which is described by the Morris-Lecar cells model. It is confirmed that successful suppression of spiral waves can be obtained by spatially clamping the membrane voltage of the excitable cells. The low voltage clamping induces breakup of spiral waves and the fragments are soon absorbed by low voltage obstacles, whereas the high voltage clamping generates travel waves that annihilate spiral waves through collision with them. However, each method has its shortcomings. Furthermore, a two-step method that combines both low and high voltage clamp techniques is then presented as a possible way of out this predicament.

  6. Electrosurgical bipolar vessel sealing versus conventional clamping and suturing for total abdominal hysterectomy: a randomized trial.

    Lakeman, M.; Kruitwagen, R.F.P.M.; Vos, M.C.; Roovers, J.P.


    STUDY OBJECTIVE: To compare the effects of bipolar vessel sealing versus conventional clamping and suturing in women undergoing total abdominal hysterectomy. DESIGN: A randomized controlled trial was performed. Patients were randomized to vessel sealing or conventional surgery. Postoperative pain wa

  7. Common voltage eliminating of SVM diode clamping three-level inverter connected to grid

    Guo, Yougui; Zeng, Ping; Zhu, Jieqiong


    A novel method of common voltage eliminating is put forward for SVM diode clamping three-level inverter connected to grid by calculation of common voltage of its various switching states. PLECS is used to model this three-level inverter connected to grid and good results are obtained. First...... analysis of common mode voltage for switching states of diode clamping 3-level inverter is given in detail. Second the common mode voltage eliminating control strategy of SVM is described for diode clamping three-level inverter. Third, PLECS is briefly introduced. Fourth, the modeling of diode clamping...... three-level inverter is presented with PLECS. Finally, a series of simulations are carried out. The simulation results tell us PLECS is a very powerful tool to real power circuits modeling. They have also verified that proposed common mode voltage eliminating control strategy of SVM is feasible...

  8. Modeling CICR in rat ventricular myocytes: voltage clamp studies

    Palade Philip T


    Full Text Available Abstract Background The past thirty-five years have seen an intense search for the molecular mechanisms underlying calcium-induced calcium-release (CICR in cardiac myocytes, with voltage clamp (VC studies being the leading tool employed. Several VC protocols including lowering of extracellular calcium to affect Ca2+ loading of the sarcoplasmic reticulum (SR, and administration of blockers caffeine and thapsigargin have been utilized to probe the phenomena surrounding SR Ca2+ release. Here, we develop a deterministic mathematical model of a rat ventricular myocyte under VC conditions, to better understand mechanisms underlying the response of an isolated cell to calcium perturbation. Motivation for the study was to pinpoint key control variables influencing CICR and examine the role of CICR in the context of a physiological control system regulating cytosolic Ca2+ concentration ([Ca2+]myo. Methods The cell model consists of an electrical-equivalent model for the cell membrane and a fluid-compartment model describing the flux of ionic species between the extracellular and several intracellular compartments (cell cytosol, SR and the dyadic coupling unit (DCU, in which resides the mechanistic basis of CICR. The DCU is described as a controller-actuator mechanism, internally stabilized by negative feedback control of the unit's two diametrically-opposed Ca2+ channels (trigger-channel and release-channel. It releases Ca2+ flux into the cyto-plasm and is in turn enclosed within a negative feedback loop involving the SERCA pump, regulating[Ca2+]myo. Results Our model reproduces measured VC data published by several laboratories, and generates graded Ca2+ release at high Ca2+ gain in a homeostatically-controlled environment where [Ca2+]myo is precisely regulated. We elucidate the importance of the DCU elements in this process, particularly the role of the ryanodine receptor in controlling SR Ca2+ release, its activation by trigger Ca2+, and its

  9. Patch Clamp: A Powerful Technique for Studying the Mechanism of Acupuncture

    D. Zhang


    Full Text Available Cellular and molecular events can be investigated using electrophysiological techniques. In particular, the patch-clamp method provides detailed information. In addition, the patch-clamp technique has become a powerful method for investigating the mechanisms underlying the effects of acupuncture. In this paper, recent researches on how acupuncture might modulate electrophysiological responses in the central nervous system (CNS and affect peripheral structures are reviewed.

  10. Delayed umbilical cord clamping after childbirth: potential benefits to baby's health

    Uwins C


    Full Text Available Christina Uwins,1 David JR Hutchon2 1Department of Obstetrics and Gynaecology, Croydon University Hospital, Croydon, 2Department of Obstetrics, Darlington Memorial Hospital, Darlington, UKAbstract: Early cord clamping was initially introduced as part of the package of care known as “active management of the third stage”, which was implemented to reduce postpartum hemorrhage. It has now been shown to provide no benefit to the mother and to result in harm to the neonate. The clinical trial evidence relating to delayed cord clamping compared to immediate cord clamping is presented and the physiological rationale for delayed cord clamping is discussed in this paper. Most organizations (eg World Health Organization (WHO, Royal College of Obstetricians and Gynaecologists (RCOG, Resuscitation Council (UK,The International Federation of Gynecology and Obstetrics (FIGO, International Confederation of Midwives, International Liaison Committee on Resuscitation (ILCOR and the European Resuscitation Council now advise a delay of 1–3 minutes before clamping the cord in term and preterm infants, and clinicians need to be aware of this change. Healthy neonates benefit from a more physiological and gentle transition from placental to pulmonary respiration, and we explain why this benefit should be provided to all neonates until there is any evidence to the contrary. The harm of early cord clamping is not limited to anemia and iron deficiency, and evidence for a wide range of possible harms of early cord clamping is presented. The need for resuscitation is one of the most common concerns, and ways of overcoming these concerns are described.Keywords: transition, cord clamping, hypovolemia, intraventricular hemorrhage

  11. Effect of non-ideal clamping shape on the resonance frequencies of silicon nanocantilevers

    Guillon, Samuel; Saya, Daisuke; Mazenq, Laurent; Nicu, Liviu [CNRS, LAAS, 7 Avenue du Colonel Roche, F-31077 Toulouse Cedex 4 (France); Perisanu, Sorin; Vincent, Pascal [LPMCN, Universite Claude Bernard Lyon 1 et CNRS, 43 boulevard du 11 novembre 1918, 69622 Villeurbanne Cedex (France); Lazarus, Arnaud; Thomas, Olivier, E-mail: [Structural Mechanics and Coupled Systems Laboratory, Conservatoire National des Arts et Metiers, 2 rue Conte, 75003 Paris (France)


    In this paper, we investigate the effects of non-ideal clamping shapes on the dynamic behavior of silicon nanocantilevers. We fabricated silicon nanocantilevers using silicon on insulator (SOI) wafers by employing stepper ultraviolet (UV) lithography, which permits a resolution of under 100 nm. The nanocantilevers were driven by electrostatic force inside a scanning electron microscope (SEM). Both lateral and out-of-plane resonance frequencies were visually detected with the SEM. Next, we discuss overhanging of the cantilever support and curvature at the clamping point in the silicon nanocantilevers, which generally arises in the fabrication process. We found that the fundamental out-of-plane frequency of a realistically clamped cantilever is always lower than that for a perfectly clamped cantilever, and depends on the cantilever width and the geometry of the clamping point structure. Using simulation with the finite-elements method, we demonstrate that this discrepancy is attributed to the particular geometry of the clamping point (non-zero joining curvatures and a flexible overhanging) that is obtained in the fabrication process. The influence of the material orthotropy is also investigated and is shown to be negligible.

  12. A High Step-Down Interleaved Buck Converter with Active-Clamp Circuits for Wind Turbines

    Chih-Lung Shen


    Full Text Available In this paper, a high step-down interleaved buck coupled-inductor converter (IBCC with active-clamp circuits for wind energy conversion has been studied. In high step-down voltage applications, an IBCC can extend duty ratio and reduce voltage stresses on active switches. In order to reduce switching losses of active switches to improve conversion efficiency, a IBCC with soft-switching techniques is usually required. Compared with passive-clamp circuits, the IBCC with active-clamp circuits have lower switching losses and minimum ringing voltage of the active switches. Thus, the proposed IBCC with active-clamp circuits for wind energy conversion can significantly increase conversion efficiency. Finally, a 240 W prototype of the proposed IBCC with active-clamp circuits was built and implemented. Experimental results have shown that efficiency can reach as high as 91%. The proposed IBCC with active-clamp circuits is presented in high step-down voltage applications to verify the performance and the feasibility for energy conversion of wind turbines.

  13. A novel monolithic piezoelectric actuated flexure-mechanism based wire clamp for microelectronic device packaging

    Liang, Cunman; Wang, Fujun; Tian, Yanling; Zhao, Xingyu; Zhang, Hongjie; Cui, Liangyu; Zhang, Dawei; Ferreira, Placid


    A novel monolithic piezoelectric actuated wire clamp is presented in this paper to achieve fast, accurate, and robust microelectronic device packaging. The wire clamp has compact, flexure-based mechanical structure and light weight. To obtain large and robust jaw displacements and ensure parallel jaw grasping, a two-stage amplification composed of a homothetic bridge type mechanism and a parallelogram leverage mechanism was designed. Pseudo-rigid-body model and Lagrange approaches were employed to conduct the kinematic, static, and dynamic modeling of the wire clamp and optimization design was carried out. The displacement amplification ratio, maximum allowable stress, and natural frequency were calculated. Finite element analysis (FEA) was conducted to evaluate the characteristics of the wire clamp and wire electro discharge machining technique was utilized to fabricate the monolithic structure. Experimental tests were carried out to investigate the performance and the experimental results match well with the theoretical calculation and FEA. The amplification ratio of the clamp is 20.96 and the working mode frequency is 895 Hz. Step response test shows that the wire clamp has fast response and high accuracy and the motion resolution is 0.2 μm. High speed precision grasping operations of gold and copper wires were realized using the wire clamper.

  14. M. tuberculosis sliding β-clamp does not interact directly with the NAD+-dependent DNA ligase.

    Vandna Kukshal

    Full Text Available The sliding β-clamp, an important component of the DNA replication and repair machinery, is drawing increasing attention as a therapeutic target. We report the crystal structure of the M. tuberculosis β-clamp (Mtbβ-clamp to 3.0 Å resolution. The protein crystallized in the space group C222(1 with cell-dimensions a = 72.7, b = 234.9 & c = 125.1 Å respectively. Mtbβ-clamp is a dimer, and exhibits head-to-tail association similar to other bacterial clamps. Each monomer folds into three domains with similar structures respectively and associates with its dimeric partner through 6 salt-bridges and about 21 polar interactions. Affinity experiments involving a blunt DNA duplex, primed-DNA and nicked DNA respectively show that Mtbβ-clamp binds specifically to primed DNA about 1.8 times stronger compared to the other two substrates and with an apparent K(d of 300 nM. In bacteria like E. coli, the β-clamp is known to interact with subunits of the clamp loader, NAD(+-dependent DNA ligase (LigA and other partners. We tested the interactions of the Mtbβ-clamp with MtbLigA and the γ-clamp loader subunit through radioactive gel shift assays, size exclusion chromatography, yeast-two hybrid experiments and also functionally. Intriguingly while Mtbβ-clamp interacts in vitro with the γ-clamp loader, it does not interact with MtbLigA unlike in bacteria like E. coli where it does. Modeling studies involving earlier peptide complexes reveal that the peptide-binding site is largely conserved despite lower sequence identity between bacterial clamps. Overall the results suggest that other as-yet-unidentified factors may mediate interactions between the clamp, LigA and DNA in mycobacteria.

  15. VLBA polarimetric observations of the CSS quasar 3C147

    Rossetti, A; Dallacasa, D; Junor, W; Salter, C J; Saikia, D J; 10.1051/0004-6361/200811190


    Aims. We report new VLBA polarimetric observations of the compact steep-spectrum (CSS) quasar 3C147 (B0538+498) at 5 and 8.4GHz. Methods. By using multifrequency VLBA observations, we derived milliarcsecond-resolution images of the total intensity, polarisation, and rotation measure distributions, by combining our new observations with archival data. Results. The source shows a one-sided structure, with a compact region, and a component extending about 200 mas to the south-west. The compact region is resolved into two main components with polarised emission, a complex rotation measure distribution, and a magnetic field dominated by components perpendicular to the source axis. Conclusions. By considering all the available data, we examine the possible location of the core component, and discuss two possible interpretations of the observed structure of this source: core-jet and lobe-hot spot. Further observations to unambiguously determine the location of the core would help distinguish between the two possibil...

  16. Spectroscopic monitoring of the Blazar 3C 454.3

    Benítez, E; Raiteri, C M; Villata, M; Dultzin, D; Martínez, O; Perez-Camargo, B; Torrealba, J


    We performed an optical spectroscopic monitoring of the blazar 3C 454.3 from September 2003 to July 2008. Sixteen optical spectra were obtained during different runs, which constitute the first spectroscopic monitoring done in the rest-frame UV region (z=0.859). An overall flux variation of the MgII (2800 A) by a factor ~ 3 was observed, while the corresponding UV continuum (F_cont at 3000 A) changed by a factor ~ 14. The MgII emission lines respond proportionally to the continuum variations when the source is in a low-activity state. In contrast, near the optical outbursts detected in 2005 and 2007, the MgII emission lines showed little response to the continuum flux variations. During the monitored period the UV FeII flux changed by a factor ~ 6 and correlated with F_cont (r = 0.92). A negative correlation between EW(Mg II) and F_cont was found, i.e. the so-called "Intrinsic Baldwin Effect".

  17. Suzaku observation of the giant radio galaxy 3C 326

    Isobe, Naoki; Gandhi, Poshak; Hayato, Asami; Nagai, Hiroshi; Hada, Kazuhiro; Seta, Hiromi; Matsuta, Keiko


    A Suzaku observation of a giant radio galaxy, 3C 326, which has a physical size of about 2 Mpc, was conducted on 2008 January 19 -- 21. In addition to several X-ray sources, diffuse emission was significantly detected associated with its west lobe, but the east lobe was contaminated by an unidentified X-ray source WARP J1552.4+2007. After careful evaluation of the X-ray and Non X-ray background, the 0.4 -- 7 keV X-ray spectrum of the west lobe is described by a power-law model. The photon index and 1 keV flux density was derived as $1.82_{-0.24}^{+0.26}\\pm0.04$ and $19.4_{-3.2}^{+3.3}\\pm 3.0$ nJy, respectively, where the first and second errors represent the statistical and systematic ones. The diffuse X-rays were attributed to be inverse Compton radiation by the synchrotron radio electrons scattering off the cosmic microwave background photons. This radio galaxy is the largest among those with lobes detected through inverse Compton X-ray emission. A comparison of the radio to X-ray fluxes yields the energy d...

  18. Integral field spectroscopy of the radio galaxy 3C 171

    Márquez, I; Durret, F; Petitjean, P


    We have performed integral field spectroscopy of the radio galaxy 3C 171 (redshift z=0.238) with the TIGER instrument at the Canada France Hawaii telescope in the Hbeta-[OIII]4959-5007 wavelength region. We present the reconstructed Hbeta and [OIII] images and compare them to the HST and radio maps. We discuss the variations of the [OIII]/Hbeta line ratio throughout the nebulosity. We also analyze the velocity field in detail, in particular the presence of several components. We find that the kinematics derived with emission lines in the central region (inside 1 arcsec) are compatible with a disk-like rotation of low amplitude (50 km/s). The continuum surface brightness profile follows an r^{1/4} law, suggesting that the underlying galaxy is an elliptical with an effective radius of 15 kpc. We have fit two components in the region centered 2.7 arcsec to the West and of extension 3 arcsec^2. We find that the blueshifted component is an extension of the central part, whereas the second one is redshifted by 600 ...

  19. 3C 273 with NuSTAR: Unveiling the AGN

    Madsen, Kristin K; Walton, Dominic J; Harrison, Fiona A; Ballantyne, David R; Boggs, Steve E; Brenneman, Laura W; Christensen, Finn E; Craig, William W; Fabian, Andrew C; Forster, Karl; Grefenstette, Brian W; Guainazzi, Matteo; Hailey, Charles J; Madejski, Greg M; Matt, Giorgio; Stern, Daniel; Zhang, William W


    We present results from a 244 ks $NuSTAR$ observation of 3C 273, obtained during a cross-calibration campaign with the $Chandra$, $INTEGRAL$, $Suzaku$, $Swift$, and $XMM-Newton$ observatories. We show that the spectrum, when fit with a power-law model using data from all observatories except $INTEGRAL$ over the 1-78 keV band, leaves significant residuals in the $NuSTAR$ data between 30-78 keV. The $NuSTAR$ 3-78 keV spectrum is well-described by an exponentially cutoff power-law ($\\Gamma = 1.646 \\pm 0.006$, E$_\\mathrm{cutoff} = 202_{-34}^{+51}$ keV) with a weak reflection component from cold, dense material. There is also evidence for a weak ($EW = 23 \\pm 11$ eV) neutral iron line. We interpret these features as arising from coronal emission plus reflection off an accretion disk or distant material. Beyond 80 keV $INTEGRAL$ data show clear excess flux relative to an extrapolation of the AGN model fit to $NuSTAR$. This high-energy power-law is consistent with the presence of a beamed jet, which begins to domina...

  20. Anisotropies in the HI gas distribution toward 3C196

    Kalberla, P M W


    The local Galactic HI gas was found to contain cold neutral medium (CNM) filaments that are aligned with polarized dust emission. These filaments appear to be dominated by the magnetic field and in this case turbulence is expected to show distinct anisotropies. We use the Galactic Effelsberg--Bonn HI Survey (EBHIS) to derive 2D turbulence spectra for the HI distribution in direction to 3C196 and two more comparison fields. Prior to Fourier transform we apply a rotational symmetric 50% Tukey window to apodize the data. We derive average as well as position angle dependent power spectra. Anisotropies in the power distribution are defined as the ratio of the spectral power in orthogonal directions. We find strong anisotropies. For a narrow range in position angle, in direction perpendicular to the filaments and the magnetic field, the spectral power is on average more than an order of magnitude larger than parallel. In the most extreme case the anisotropy reaches locally a factor of 130. Anisotropies increase on...

  1. Heavy oil recovery process: Conceptual engineering of a downhole methanator and preliminary estimate of facilities cost for application to North Slope Alaska

    Gondouin, M.


    The West Sak (Upper Cretaceous) sands, overlaying the Kuparuk field, would rank among the largest known oil fields in the US, but technical difficulties have so far prevented its commercial exploitation. Steam injection is the most successful and the most commonly-used method of heavy oil recovery, but its application to the West Sak presents major problems. Such difficulties may be overcome by using a novel approach, in which steam is generated downhole in a catalytic Methanator, from Syngas made at the surface from endothermic reactions (Table 1). The Methanator effluent, containing steam and soluble gases resulting from exothermic reactions (Table 1), is cyclically injected into the reservoir by means of a horizontal drainhole while hot produced fluids flow form a second drainhole into a central production tubing. The downhole reactor feed and BFW flow downward to two concentric tubings. The large-diameter casing required to house the downhole reactor assembly is filled above it with Arctic Pack mud, or crude oil, to further reduce heat leaks. A quantitative analysis of this production scheme for the West Sak required a preliminary engineering of the downhole and surface facilities and a tentative forecast of well production rates. The results, based on published information on the West Sak, have been used to estimate the cost of these facilities, per daily barrel of oil produced. A preliminary economic analysis and conclusions are presented together with an outline of future work. Economic and regulatory conditions which would make this approach viable are discussed. 28 figs.

  2. The effect of high glucose levels on the hypermethylation of protein phosphatase 1 regulatory subunit 3C (PPP1R3C) gene in colorectal cancer

    Soo Kyung Lee; Ji Wook Moon; Yong Woo Lee; Jung Ok Lee; Su Jin Kim; Nami Kim; Jin Kim; Hyeon Soo Kim; Sun-Hwa Park


    DNA methylation is an epigenetic event that occurs frequently in colorectal cancer (CRC). Increased glucose level is a strong risk factor for CRC. Protein phosphatase 1 regulatory subunit 3C (PPP1R3C) modulates glycogen metabolism, particularly glycogen synthesis. The aim of this study was to investigate the effect of high glucose levels on DNA methylation of PPP1R3C in CRC. PPP1R3C was significantly hypermethylated in CRC tissues (76/105, 72.38%, < 0.05) and colon cancer cell lines ( < 0.05). CRC tissues obtained from patients with high glucose levels showed that the methylation of PPP1R3C was lower than in patients who had normal levels of glucose. When DLD-1 cells were cultured under conditions of high glucose, the methylation of PPP1R3C was repressed. The expression of PPP1R3C was inversely related to methylation status. In addition, a promoter luciferase assay showed that the transcriptional activity of PPP1R3C was increased in high glucose culture conditions. The number of cells decreased when PPP1R3C was silenced in DLD-1 cells. These results suggest that PPP1R3C, a novel hypermethylated gene in CRC, may play a critical role in cancer cell growth in association with glucose levels.

  3. A central swivel point in the RFC clamp loader controls PCNA opening and loading on DNA.

    Sakato, Miho; O'Donnell, Mike; Hingorani, Manju M


    Replication factor C (RFC) is a five-subunit complex that loads proliferating cell nuclear antigen (PCNA) clamps onto primer-template DNA (ptDNA) during replication. RFC subunits belong to the AAA(+) superfamily, and their ATPase activity drives interactions between the clamp loader, the clamp, and the ptDNA, leading to topologically linked PCNA·ptDNA. We report the kinetics of transient events in Saccharomyces cerevisiae RFC-catalyzed PCNA loading, including ATP-induced RFC activation, PCNA opening, ptDNA binding, ATP hydrolysis, PCNA closing, and PCNA·ptDNA release. This detailed perspective enables assessment of individual RFC-A, RFC-B, RFC-C, RFC-D, and RFC-E subunit functions in the reaction mechanism. Functions have been ascribed to RFC subunits previously based on a steady-state analysis of 'arginine-finger' ATPase mutants; however, pre-steady-state analysis provides a different view. The central subunit RFC-C serves as a critical swivel point in the clamp loader. ATP binding to this subunit initiates RFC activation, and the clamp loader adopts a spiral conformation that stabilizes PCNA in a corresponding open spiral. The importance of RFC subunit response to ATP binding decreases as RFC-C>RFC-D>RFC-B, with RFC-A being unnecessary. RFC-C-dependent activation of RFC also enables ptDNA binding, leading to the formation of the RFC·ATP·PCNA(open)·ptDNA complex. Subsequent ATP hydrolysis leads to complex dissociation, with RFC-D activity contributing the most to rapid ptDNA release. The pivotal role of the RFC-B/C/D subunit ATPase core in clamp loading is consistent with the similar central location of all three ATPase active subunits of the Escherichia coli clamp loader.

  4. Multiwavelength Observations of 3C66A in 2003

    Boettcher, M.; Joshi, M.; Fossati, G.; Smith, I. A.; Mukherjee, R.; Bramel, D.; Cui, W.; WEBT Collaboration


    The radio-selected BL Lac object 3C66A was the target of an intensive multiwavelength observing campaign in the last quarter of 2003 and early 2004. It was monitored by the Whole Earth Blazar Telescope (WEBT) collaboration of optical observers, in tandem with 20 X-ray monitoring observations by the Rossi X-Ray Timing Explorer (RXTE), VHE gamma-ray observations by STACEE and VERITAS, and long-term monitoring at radio frequencies. In addition, 9 high-spatial-resolution observations using the VLA are being carried out during the campaign and throughout the year 2004 to follow possible structural changes of the source. A gradual brightening of the source over the course of the campaign was observed at all optical frequencies, culminating in a very bright flare at the end of January 2004. Optical light curves indicate intraday microvariability on time scales down to about 1.3 hours. No significant color-magnitude correlation for the entire data set was evident, but there is a slight indication of a hardness - intensity anti-correlation on intraday time scales. The X-ray spectrum is consistent with a power-law with a photon spectral index of ˜ 2.1, indicating that the RXTE energy band might be located right at the intersection of the synchrotron and the high-energy emission components of the broadband spectral energy distribution. No significant flux or spectral variability at X-ray energies was detected. We extracted snapshot spectral energy distributions at various times throughout the campaign, and present first spectral fits to those SEDs. This work was partially supported by NASA RXTE GO grant no. NNG 04GB13G.

  5. Investigating the X-ray and Gamma-ray Properties of the Galactic Supernova Remnants Kes 69, 3C 396, 3C 400.2

    Ergin, Tülün; Yamazaki, Ryo


    Kes 69, 3C 396, and 3C 400.2 are mixed-morphology (MM) Galactic supernova remnants (SNRs), where Kes 69 and 3C 396 are interacting with molecular clouds (MCs). Previous X-ray studies showed that the emission from these SNRs is thermal. It has been suggested that MM SNRs interacting with MCs are potential candidates for recombining plasma (RP) in X-rays and hadronic gamma-ray emission. Recently, Chandra observations revealed signs of RP in 3C 400.2. Our preliminary analyses show that the X-ray emission of NW and SE region of 3C 400.2 arises from recombining plasma. We detected GeV gamma-ray emission from Kes 69 and 3C 396 above 5$\\sigma$.

  6. Retrieval of Ocean Bottom and Downhole Seismic sensors orientation using integrated MEMS gyroscope and direct rotation measurements

    D'Alessandro, A.; D'Anna, G.


    The absolute orientation of the horizontal components of ocean bottom or downhole seismic sensors are generally unknown. Almost all the methods proposed to overcome this issue are based on the post-processing of the acquired signals and so the results are strongly dependent on the nature, quantity and quality of the acquired data. We have carried out several test to evaluate the ability of retrieve sensor orientation using integrated low cost MEMS gyroscope. Our tests have shown that the tested MEMS gyroscope (the model 1044_0-3/3/3 Phidget Spatial Precision High Resolution) can be used to measure angular displacement and therefore to retrieve the absolute orientation of the horizontal components of a sensor that has been subjected to rotation in the horizontal plane. A correct processing of the acquired signals permit to retrieve, for rotation at angular rate between 0 and 180° s-1, angular displacement with error less 2°.

  7. Continuous record of geomagnetic field intensity between 4.7 and 2.7 Ma from downhole measurements

    Thibal, J.; Pozzi, J.-P.; Barthès, V.; Dubuisson, G.


    A continuous record of the geomagnetic field intensity from 4.7 to 2.7 Ma has been obtained, together with a precise magnetostratigraphy, from downhole magnetic measurements at Site 884 of ODP Leg 145 in the North Pacific. The record confirms the saw-tooth pattern of geomagnetic field intensity proposed by Valet et Meynadier [10]. Reversals are characterized by a steep intensity decrease followed by a quick regeneration. Over each polarity interval, rapid variations are superimposed over a progressive decrease in the mean intensity. We find that the duration of each polarity interval is inversely proportional to the mean rate of decrease in the field intensity over this period, and thus this duration seems to be pre-determined.

  8. Clampe de Ganz no tratamento de urgência em lesões do anel pélvico Ganz clamps to treat emergencies in pelvic ring lesions

    Gilberto José Cação Pereira


    Full Text Available OBJETIVO: Avaliar a eficiência do clampe de Ganz na estabilização e redução dos deslocamentos da pelve, quando utilizado no tratamento de urgência, além de aquilatar eventuais dificuldades e facilidades do método. MÉTODOS: O clampe de Ganz foi utilizado no tratamento de urgência em 31 pacientes com graves lesões do anel pélvico (Tile C associadas a importante instabilidade hemodinâmica. RESULTADO: Entre os pacientes, 27 (87,1% apresentaram evolução favorável, com estabilização, redução dos deslocamentos e compressão da região posterior do anel, além de estabilização hemodinâmica, e quatro (12,9% evoluíram para óbito. CONCLUSÃO: O clampe de Ganz mostrou-se eficiente ferramenta no tratamento de urgência das lesões do anel pélvico, por ser de concepção simples, de rápida colocação, não impedir ou dificultar procedimentos no abdome e, principalmente, por permitir a estabilização da pelve, redução dos deslocamentos e compressão na região posterior do anel, local onde ocorrem os maiores sangramentos, reduzindo-os ou eliminando-os.OBJECTIVE: To evaluate the effectiveness of Ganz clamp to stabilize and reduce pelvic dislocations in emergency situations, and to assess possible difficult and easy aspects of the method. METHOD: Ganz clamp was used in the emergency treatment of 31 patients with severe lesions of the pelvic ring (Tile C associated to major hemodynamic instability. RESULTS: Among the patients, 27 (87.1% presented a favorable evolution, with stabilization, reduction of dislocations, and compression of the posterior portion of the ring, and also hemodynamic stabilization, and 4 (12.9% died. CONCLUSION: The Ganz clamp was found to be an effective tool in the emergency treatment of pelvic ring lesions because it has an uncomplicated conception, allowing for a fast placement, because it does not prevent nor make it difficult for abdominal procedures, and most of all because it allows for the

  9. Incision Below The Clamp sebagai Modifikasi Teknik Insisi pada Frenektomi untuk Minimalisasi Perdarahan

    S. Suryono


    Full Text Available Latar Belakang. Perlekatan frenulum tinggi berdampak merugikan bagi jaringan periodontal maupun fungsi estetik. Perlekatan tinggi yang terjadi pada labialis superior, menimbulkan gingivitis dan sentral diastema yang menjadikan indikasi untuk dilakukan frenektomi. Frenektomi dengan teknik melakukan insisi di atas dan di bawah clamp berakibat lebarnya luka karena tarikan otot bibir, yang berdampak terjadi banyak perdarahan. Tujuan. Melakukan evaluasi terhadap modifikasi teknik insisi yang diharapkan dapat mengurangi perdarahan. Kasus dan Penanganan. Laporan kasus ini memaparkan penanganan kasus pada perlekatan frenulum tinggi yang diikuti adanya sentral diastema. Pasien dengan diagnosa frenulum tinggi dan indikasi frenektomi dilakukan tindakan preoperasi. Tindakan operasi dilakukan dengan menggunakan pisau bedah, modifikasi insisi (Insision below the Clamp dilakukan dengan cara menempatkan clamp frenulum pada posisi yang berdekatan dan sejajar dengan bibir, tindakan insisi dilakukan di bawah clamp, disusul dengan penjahitan pada area mucolabial fold. Selama operasi dilakukan terlihat luka tidak melebar, tidak banyak darah yang keluar, pasien dan dokter merasa nyaman. Kesimpulan. Insision below the Clamp merupakan modifikasi teknik insisi pada frenektomi yang bisa dilakukan oleh para praktisi untuk meminimalisir perdarahan yang terjadi selama proses pengambilan frenulum dengan menggunakan pisau bedah.   Background. Attachment of high frenulum has negative impact on periodontal tissues as well as an aesthetic function. High attachment of frenulum that occurs in labialis superior, causing gingivitis and central diastema that are indications to do Frenectomy. Conventional techniques of Frenectomy performed with insisions above and below the clamp resulted in wide injury due to muscle contraction, which affects a lot of bleeding. Objective. To evaluate modification insision technique which hopefully can minimize the bleeding. Case and Treatment. This

  10. Down-hole magnetometric resistivity inversion for zinc and lead lenses localization at tobermalug, county Limerick, Ireland

    Abderrezak, Bouchedda; Bernard, Giroux; Michel, Allard


    The magnetometric resistivity (MMR) method consists of measuring the variation of magnetostatic field associated to current density variations. It is based on the principle that direct current flow between two electrodes takes the path of least resistance through the earth. Consequently, conductive or relatively conductive ore bodies such as massive, semi-massive to disseminated sulphides are preferentially energized, which increases the current density in their vicinity. MMR is more efficient than electromagnetic methods in the case of weak conductivity contrasts. To improve the signal to noise ratio the magnetic field can be measured in boreholes rather than on the surface. Down-hole magnetometric resistivity measurements have been conducted in Tobermalug prospect in County Limerick, Ireland. The survey was used as an alternative to down-hole electromagnetic to delineate subhorizontal zinc/lead mineralization lenses that can be poorly conductive. Two survey areas were investigated, DHMMR1 and DHMMR2. Interpretation is based on the regularized least-squares inversion of MMR data, in which the MMR forward problem is solved with a finite volume discretization of the electrostatic and magnetostatic equations. Inversion of synthetic data generated over two conductive horizontal discs model shows that conductive structures are well positioned but their extensions is biased along current electrodes orientation. Inversion of the field data allowed localizing a few conductive elongated targets. At DHMMR1, the conductivity is weaker and seems to be associated to disseminated mineralization. DHMMR2 contains a higher conductivity and more elongated target. It appears to be associated to semi-massive sulphides.

  11. Novel Low Loss Active Voltage Clamp Circuit for Series Connection of RCGCT thyristors

    Ito, Hiroshi; Suzuki, Akihiro; Iwata, Akihiko

    This paper describes novel low loss active voltage clamp circuits for the series connection of RCGCT thyristors. For high voltage converters the series connection of power semiconductor devices is an essential technique for direct switching of high voltages. Several protection circuits have been applied to the series connection of RCGCT thyristors such as CRD snubber circuits which suppress over-voltages across RCGCT thyristors, and voltage balancing resistors to equalize voltage sharing in steady states. However, significant losses in these protection circuits lower the converter’s efficiency. We propose novel low-loss protection circuits, which have active voltage clamp snubber circuits and static voltage balancing circuits. The clamp capacitor voltage of the active voltage clamp snubber circuits are designed to be higher than the equally divided DC-Link voltage. This method can reduce the loss of the clamp circuit to no more than 1/10 of that of the conventional CRD snubber. Also the static voltage balancing circuits compensate for the voltage imbalance generated by the difference in the leakage current between the series connection RCGCT thyristors.

  12. Gain-clamping techniques in two-stage double-pass L-band EDFA

    S W harun; N Md Samsuri; H Ahmad


    Two designs of long-wavelength band erbium-doped fiber amplifier (L-band EDFA) for gain clamping in double-pass systems are demonstrated and compared. The first design is based on ring laser technique where a backward amplified spontaneous emission (ASE) from the second stage is routed into the feedback loop to create an oscillating laser for gain clamping. The gain is clamped at 18.6 dB from -40 to -8 dBm with a gain variation of less than ± 0.1 dB and a noise figure of less than 6 dB. Another scheme is based on partial reflection of ASE into the EDFA, which is demonstrated using a narrowband fiber Bragg grating. This scheme achieves a good gain clamping characteristic up to -12 dBm of input signal power with a gain variation of less than ± 0.3 dB from a clamped gain of 22 dB. The noise figure of a 1580 nm signal is maintained below 5 dB in this amplifier since this scheme is not based on lasing mechanism. The latter scheme is also expected to be free from the relaxation oscillation problem.

  13. Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience

    Can eTao


    Full Text Available Neuronal activity is dominated by synaptic inputs from excitatory or inhibitory neural circuits. With the development of in vivo patch-clamp recording, especially in vivo voltage-clamp recording, researchers can not only directly measure neuronal activity, such as spiking responses or membrane potential dynamics, but also quantify synaptic inputs from excitatory and inhibitory circuits in living animals. This approach enables researchers to directly unravel different synaptic components and to understand their underlying roles in particular brain functions. Combining in vivo patch-clamp recording with other techniques, such as two-photon imaging or optogenetics, can provide even clearer functional dissection of the synaptic contributions of different neurons or nuclei. Here, we summarized current applications and recent research progress using the in vivo patch-clamp recording method and focused on its role in the functional dissection of different synaptic inputs. The key factors of a successful in vivo patch-clamp experiment and possible solutions based on references and our experiences were also discussed.

  14. Development and Test of a 1,000 Level 3C Fiber Optic Borehole Seismic Receiver Array Applied to Carbon Sequestration

    Paulsson, Bjorn N.P. [Paulsson, Inc., Van Nuys, CA (United States)


    To address the critical site characterization and monitoring needs for CCS programs, US Department of Energy (DOE) awarded Paulsson, Inc. in 2010 a contract to design, build and test a fiber optic based ultra-large bandwidth clamped borehole seismic vector array capable of deploying up to one thousand 3C sensor pods suitable for deployment into high temperature and high pressure boreholes. Paulsson, Inc. has completed a design or a unique borehole seismic system consisting of a novel drill pipe based deployment system that includes a hydraulic clamping mechanism for the sensor pods, a new sensor pod design and most important – a unique fiber optic seismic vector sensor with technical specifications and capabilities that far exceed the state of the art seismic sensor technologies. These novel technologies were all applied to the new borehole seismic system. In combination these technologies will allow for the deployment of up to 1,000 3C sensor pods in vertical, deviated or horizontal wells. Laboratory tests of the fiber optic seismic vector sensors developed during this project have shown that the new borehole seismic sensor technology is capable of generating outstanding high vector fidelity data with extremely large bandwidth: 0.01 – 6,000 Hz. Field tests have shown that the system can record events at magnitudes much smaller than M-2.3 at frequencies up to 2,000 Hz. The sensors have also proved to be about 100 times more sensitive than the regular coil geophones that are used in borehole seismic systems today. The fiber optic seismic sensors have furthermore been qualified to operate at temperatures over 300°C (572°F). The fibers used for the seismic sensors in the system are used to record Distributed Temperature Sensor (DTS) data allowing additional value added data to be recorded simultaneously with the seismic vector sensor data.


    HU Yu-da; QIU Jia-jun; TA Na


    Vibration problems of a segment of winding between two clamping plates are studied when the clamping plates, which are used to fix stator end winding, are loose. First,magnetic induction expressions of the winding while the generator was running were given by using separation of variables method. Also, the expressions of the winding electromagnetic force and dry friction force between loosing clamping plates were gotten. Secondly, a mechanical model, which was used to study nonlinear vibration problem of the winding, was set up. Fundamental resonance was analyzed by using multiple scales method, and a resonance equation of amplitude and frequency in steady state was given. Then stability,bifurcation and singularity of the steady solution were studied. Criterions of stability and transition set of the bifurcation equation were obtained. At last, through numerical calculations, resonance curves were obtained. The results are helpful for analysis and protection of generator accidents.

  16. Minimum Normal Force Principle Based Quantitive Optimizationof Clamping Forces for Thin Walled Part

    ZHANG Fa-ping; SUN Hou-fang; SHAHID I.Butt


    Based on the stability criteria of workpiece-fixture system, quantitative optimization of clamping forces during precise machining process for thin walled part is studied considering the contact condition between wokpiece and locator, the contact mechanical model is achieved ,which is further been used to calculate the entire passive forces acting on the statically undetermined workpiece by means of the force screw theory as well as minimum norm force principle. Furthermore, a new methodology to optimize clamping forces is put forward, on the criteria of keeping the stability of workpiece during cutting process. By this way, the intensity of clamping forces is decreased dramatically, which will be most beneficial for improving the machining quality of thin-walled parts. Finally, a case study is used to support and validate the proposed model.

  17. Sequential dissection of multiple ionic currents in single cardiac myocytes under action potential-clamp.

    Banyasz, Tamas; Horvath, Balazs; Jian, Zhong; Izu, Leighton T; Chen-Izu, Ye


    The cardiac action potential (AP) is shaped by myriad ionic currents. In this study, we develop an innovative AP-clamp Sequential Dissection technique to enable the recording of multiple ionic currents in the single cell under AP-clamp. This new technique presents a significant step beyond the traditional way of recording only one current in any one cell. The ability to measure many currents in a single cell has revealed two hitherto unknown characteristics of the ionic currents in cardiac cells: coordination of currents within a cell and large variation of currents between cells. Hence, the AP-clamp Sequential Dissection method provides a unique and powerful tool for studying individual cell electrophysiology.

  18. Enhancing endogenous stem cells in the newbornvia delayed umbilical cord clamping

    Christopher Lawton; Sandra Acosta; Nate Watson; Chiara Gonzales-Portillo; hTeo Diamandis; Naoki Tajiri; Yuji Kaneko; Paul R. Sanberg; Cesar V. Borlongan


    There is currently no consensus among clinicians and scientists over the appropriate or optimal timing for umbilical cord clamping. However, many clinical studies have suggested that delayed cord clamping is associated with various neonatal beneifts including increased blood volume, reduced need for blood transfusion, increased cerebral oxygenation in pre-term infants, and decreased frequency of iron deifciency anemia in term infants. Human umbilical cord blood con-tains signiifcant amounts of stem and progenitor cells and is currently used in the treatment of several life-threatening diseases. We propose that delayed cord clamping be encouraged as it en-hances blood lfow from the placenta to the neonate, which is accompanied by an increase supply of valuable stem and progenitor cells, as well as may improve blood oxygenation and increase blood volume, altogether reducing the infant’s susceptibility to both neonatal and age-related diseases.

  19. Modified Blalock clamp: a single-hand autostatic device for pulmonary vessel occlusion during lung cancer resection

    Petrella, Francesco; Solli, Piergiorgio; Borri, Alessandro; Spaggiari, Lorenzo


    Vascular clamping during lung cancer resection may be difficult in patients with short vessels or large neoplasms preventing adequate and safe exposure. In addition, the physiological vicinity of some vessels to rigid structures like the bronchi may interfere with ideal clamp positioning even in ordinary procedures. We have modified the original Blalock clamp to facilitate the control of pulmonary vessels and physiologically compress the vessel walls, thereby allowing optimal vascular resecti...

  20. Comparison of herniorrhaphy versus clamping of umbilical hernias in horses: a retrospective study of 93 cases (1982-1994).


    Many uncomplicated umbilical hernias have been managed successfully in foals by the application of a hernia clamp. Isolated reports of complications following clamp application have led some authors to suggest that it is an unsuitable method of treatment. Little information has been published comparing the complication rates associated with the use of hernia clamps and herniorrhaphy in the treatment of umbilical hernias. The purpose of this retrospective study was to report the characteristic...

  1. Multi-Frequency VLBA Studies of the Parsec-Scale Jets in 3C 66A and 3C 66B

    G.-Y. Zhao; Y.-J. Chen; Z.-Q. Shen; H. Sudou; S. Iguchi; F. Gao; Y. Murata; Y. Taniguchi


    We report multi-frequency VLBA phase-referencing observation results of 3C 66A and 3C 66B, including high resolution maps and relative position measurements. The resulting images show similar morphology with that presented in previous works. We find core shift variations in both sources, indicating some physical condition changes in the jets.

  2. Can robots patch-clamp as well as humans? Characterization of a novel sodium channel mutation.

    Estacion, M; Choi, J S; Eastman, E M; Lin, Z; Li, Y; Tyrrell, L; Yang, Y; Dib-Hajj, S D; Waxman, S G


    Ion channel missense mutations cause disorders of excitability by changing channel biophysical properties. As an increasing number of new naturally occurring mutations have been identified, and the number of other mutations produced by molecular approaches such as in situ mutagenesis has increased, the need for functional analysis by patch-clamp has become rate limiting. Here we compare a patch-clamp robot using planar-chip technology with human patch-clamp in a functional assessment of a previously undescribed Nav1.7 sodium channel mutation, S211P, which causes erythromelalgia. This robotic patch-clamp device can increase throughput (the number of cells analysed per day) by 3- to 10-fold. Both modes of analysis show that the mutation hyperpolarizes activation voltage dependence (8 mV by manual profiling, 11 mV by robotic profiling), alters steady-state fast inactivation so that it requires an additional Boltzmann function for a second fraction of total current (approximately 20% manual, approximately 40% robotic), and enhances slow inactivation (hyperpolarizing shift--15 mV by human,--13 mV robotic). Manual patch-clamping demonstrated slower deactivation and enhanced (approximately 2-fold) ramp response for the mutant channel while robotic recording did not, possibly due to increased temperature and reduced signal-to-noise ratio on the robotic platform. If robotic profiling is used to screen ion channel mutations, we recommend that each measurement or protocol be validated by initial comparison to manual recording. With this caveat, we suggest that, if results are interpreted cautiously, robotic patch-clamp can be used with supervision and subsequent confirmation from human physiologists to facilitate the initial profiling of a variety of electrophysiological parameters of ion channel mutations.

  3. A Two-Dimensional Zirconium Carbide by Selective Etching of Al3C3 from Nanolaminated Zr3Al3C5.

    Zhou, Jie; Zha, Xianhu; Chen, Fan Y; Ye, Qun; Eklund, Per; Du, Shiyu; Huang, Qing


    The room-temperature synthesis of a new two-dimensional (2D) zirconium-containing carbide, Zr3C2T(z) MXene is presented. In contrast to traditional preparation of MXene, the layered ternary Zr3Al3C5 material instead of MAX phases is used as source under hydrofluoric acid treatment. The structural, mechanical, and electronic properties of the synthesized 2D carbide are investigated, combined with first-principles density functional calculations. A comparative study on the structrual stability of our obtained 2D Zr3C2T(z) and Ti3C2T(z) MXenes at elevated temperatures is performed. The obtained 2D Zr3C2T(z) exhibits relatively better ability to maintain 2D nature and strucural integrity compared to Ti-based Mxene. The difference in structural stability under high temperature condition is explained by a theoretical investigation on binding energy.

  4. Design and fabrication of a planar patch-clamp substrate using a silicon-on-insulator wafer

    Zhenlong, Zhang; Xiangyang, Liu; Yanli, Mao


    The planar patch-clamp technique has been applied to high throughput screening in drug discovery. The key feature of this technique is the fabrication of a planar patch-clamp substrate using appropriate materials. In this study, a planar patch-clamp substrate was designed and fabricated using a silicon-on-insulator (SOI) wafer. The access resistance and capacitance of SOI-based planar patch-clamp substrates are smaller than those of bulk silicon-based planar substrates, which will reduce the distributed RC noise.

  5. Discussion on compact mechanism of air-stream and synchro-formed clamp plate impact molding

    Zhenling WANG


    Full Text Available Applying the air impact molding method to mold the complicated pattern with wider opening surface and deeper concave, there always exist vaulted phenomenon and lower compactibility of sand mold over the entrance and the concave regions. Using the air-stream and synchro-formed clamp plate impact molding, however, this problem will be preferably solved. In this paper, the compact mechanism of the new molding method and the effect of some configuration factors, such as the area flowed by compressed air and the highness of the protruding block displacement around the diffluent clamp plate, on the compactibility of sand mold were discussed.

  6. Experience of application of clamp-on cross-correlation flow meter in nuclear industry

    Gurevich, Y. [Daystar Technology Inc., Ontario (Canada); Ton, V.; Kotenyov, S.; Zhao, C. [Ontario Power Generation, Ontario (Canada); Sharp, B.; Lopez, A. [Advanced Measurements and Analysis Group Inc., Ontario (Canada)


    The cross-correlation clamp-on flow meter, CROSSFLOW, developed and manufactured by AMAG, has been used over the world for over 15 years for flow measurements in various systems in nuclear and fossil power plants. Prior that, OPG has used similar technology in Canadian nuclear power plants since 1980-ies. Two recent examples of the application of the clamp-on cross-correlation technology are presented in this paper. In first example OPG meter was used to verify accuracy of ASME nozzles installed in condensate flow lines. In second example AMAG meter was used to measure Diesel Cooling Water (DCW). (author)

  7. Clamping drainage is unnecessary after minimally invasive total knee arthroplasty in patients with tranexamic acid

    Wu, Yuangang; Yang, Timin; Zeng, Yi; Li, Canfeng; Shen, Bin; Pei, Fuxing


    Abstract Background: Drainage and tranexamic acid (TXA) have been widely used in total knee arthroplasty (TKA). However, it remains unclear whether it is necessary to clamp the drain after minimally invasive TKA (MIS-TKA) when TXA is used. We therefore conducted a randomized controlled trial to compare the effects of clamping versus not clamping drainage following MIS-TKA in patients in whom TXA was used. Methods: From January 2015 to December 2015, 121 patients undergoing unilateral primary MIS-TKA were enrolled and randomly divided into 2 groups. In the clamping group (N = 60), drainage was clamped for the 1st 4 postoperative hours. In the nonclamping group (N = 61), drainage was not clamped. All patients underwent a minimidvastus approach and received 10 mg/kg TXA intravenously before tourniquet deflation. We recorded the total blood loss, drainage volume, and transfusion requirements in the postoperative period. We also measured the hemoglobin (Hb) and hematocrit (Hct) levels on postoperative days 1, 3, and 5. Other factors, including range of motion (ROM), visual analog scale (VAS), and occurrence of wound-related complications, deep vein thrombosis (DVT), and pulmonary embolism (PE) were recorded at the time of discharge and 1 and 6 months postoperatively. No statistically significant differences were found between the 2 groups with regard to age, gender, weight, BMI, preoperative Hb and Hct levels, preoperative ROM, VAS, duration of surgery, anesthesia method, and the American Society of Anesthesiologists classification. Results: The clamping group experienced better drainage volume results than the nonclamping group (P < 0.001). There were no statistically significant differences in TBL and transfusion requirements (P = 0.105 and 0.276, respectively); Hb and Hct levels on postoperative days 1, 3, and 5 were similar between the 2 groups. No significant differences were found for ROM, VAS, DVT, PE, wound-related complications, and hospital

  8. Cerebral oxygenation and processed EEG response to clamping and shunting during carotid endarterectomy under general anesthesia.

    Perez, William; Dukatz, Christopher; El-Dalati, Sami; Duncan, James; Abdel-Rasoul, Mahmoud; Springer, Andrew; Go, Michael R; Dzwonczyk, Roger


    Clamping and shunting during carotid endarterectomy (CEA) surgery causes changes in cerebral blood flow. The purpose of this study was to assess and compare, side by side, the cerebral oxygenation (rSO2) and processed electroencephalogram (EEG) response bilaterally to carotid artery clamping and shunting in patients undergoing CEA under general anesthesia. With institutional approval and written informed consent, patients undergoing CEA under general anesthesia and routine carotid artery shunting were recorded bilaterally, simultaneously and continuously with an rSO2 and processed EEG monitor. The response of the monitors during carotid artery clamping and shunting were assessed and compared between monitors and bilaterally within each monitor. Sixty-nine patients were included in the study. At clamping the surgical-side and contralateral-side rSO2 dropped significantly below the baseline incision value (-17.6 and -9.4% respectively). After shunting, the contralateral-side rSO2 returned to baseline while the surgical-side rSO2 remained significantly below baseline (-9.0%) until the shunt was removed following surgery. At clamping the surgical-side and contralateral-side processed EEG also dropped below baseline (-19.9 and -20.6% respectively). However, following shunt activation, the processed EEG returned bilaterally to baseline. During the course of this research, we found the rSO2 monitor to be clinically more robust (4.4% failure rate) than the processed EEG monitor (20.0% failure rate). There was no correlation between the rSO2 or processed EEG changes that occurred immediately after clamping and the degree of surgical side stenosis measured pre-operatively. Both rSO2 and processed EEG respond to clamping and shunting during CEA. Cerebral oximetry discriminates between the surgical and contralateral side during surgery. The rSO2 monitor is more reliable in the real-world clinical setting. Future studies should focus on developing algorithms based on these

  9. In vivo patch clamp recording technique in the study of neurophysiology

    Lan-Wei CUI; Yu-Rong LI


    The patch clamp recording technique in vivois a blind patch clamp recording methods to record the current of the spinal or cereral neurons of anaesthesia ( or awake) animals. This technique can be used to study the synaptic function and plasticity in central nervous system in vivoin order to understand the physiological properties of the ion channels from an integrated point of view. The advantage of this technique have already presented itself in the study of the synaptic transmission and nervous network. Nowadays, in vivo patch whole-cell recording technique in combination with other techniques is becoming a common method in the research fields.

  10. 18 CFR 3c.3 - Reporting fraud, waste, abuse, and corruption and cooperation with official inquiries.


    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Reporting fraud, waste, abuse, and corruption and cooperation with official inquiries. 3c.3 Section 3c.3 Conservation of Power... OF CONDUCT § 3c.3 Reporting fraud, waste, abuse, and corruption and cooperation with...

  11. A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode

    Jergic, Slobodan; Horan, Nicholas P.; Elshenawy, Mohamed M.; Mason, Claire E.; Urathamakul, Thitima; Ozawa, Kiyoshi; Robinson, Andrew; Goudsmits, Joris M. H.; Wang, Yao; Pan, Xuefeng; Beck, Jennifer L.; van Oijen, Antoine M.; Huber, Thomas; Hamdan, Samir M.; Dixon, Nicholas E.


    Processive DNA synthesis by the alpha epsilon theta core of the Escherichia coli Pol III replicase requires it to be bound to the beta(2) clamp via a site in the a polymerase subunit. How the epsilon proofreading exonuclease subunit influences DNA synthesis by alpha was not previously understood. In

  12. Pulse and Trapezoidal Voltage Clamp Applied To Jurkat Cells: A T- Lymphocyte Cell Line


    clamp. (Upward deflection represents depolarization). Low frequency oscillations are clearly visible. Such oscillations have been described by Maltsev ...1952. (8) Maltsev , V. A. Oscillating and triggering properties of T cell membrane potential. Imun. Lett. 26:277-282, 1990. 13 (9) Gallin, E. K

  13. Two-Dimensional Fiber Positioning and Clamping Device for Product-Internal Microassembly

    Henneken, V.A.; Sassen, W.P.; Van der Vlist, W.; Wien, W.H.A.; Tichem, M.; Sarro, P.M.


    In this paper, we present a microelectromechanical systems-based two-degrees-of-freedom positioning device combined with a clamping structure for positioning and constraining an optical fiber. The fiber position can be controlled in the two directions perpendicular to the fiber axis using two specif

  14. A Single-Strand Annealing Protein Clamps DNA to Detect and Secure Homology.

    Marcel Ander


    Full Text Available Repair of DNA breaks by single-strand annealing (SSA is a major mechanism for the maintenance of genomic integrity. SSA is promoted by proteins (single-strand-annealing proteins [SSAPs], such as eukaryotic RAD52 and λ phage Redβ. These proteins use a short single-stranded region to find sequence identity and initiate homologous recombination. However, it is unclear how SSAPs detect homology and catalyze annealing. Using single-molecule experiments, we provide evidence that homology is recognized by Redβ monomers that weakly hold single DNA strands together. Once annealing begins, dimerization of Redβ clamps the double-stranded region and nucleates nucleoprotein filament growth. In this manner, DNA clamping ensures and secures a successful detection for DNA sequence homology. The clamp is characterized by a structural change of Redβ and a remarkable stability against force up to 200 pN. Our findings not only present a detailed explanation for SSAP action but also identify the DNA clamp as a very stable, noncovalent, DNA-protein interaction.

  15. Critical clamp loader processing by an essential AAA+ protease in Caulobacter crescentus.

    Vass, Robert H; Chien, Peter


    Chromosome replication relies on sliding clamps that are loaded by energy-dependent complexes. In Escherichia coli, the ATP-binding clamp loader subunit DnaX exists as both long (τ) and short (γ) forms generated through programmed translational frameshifting, but the need for both forms is unclear. Here, we show that in Caulobacter crescentus, DnaX isoforms are unexpectedly generated through partial proteolysis by the AAA+ protease casein lytic proteinase (Clp) XP. We find that the normally processive ClpXP protease partially degrades DnaX to produce stable fragments upon encountering a glycine-rich region adjacent to a structured domain. Increasing the sequence complexity of this region prevents partial proteolysis and generates a τ-only form of DnaX in vivo that is unable to support viability on its own. Growth is restored when γ is provided in trans, but these strains are more sensitive to DNA damage compared with strains that can generate γ through proteolysis. Our work reveals an unexpected mode of partial processing by the ClpXP protease to generate DnaX isoforms, demonstrates that both τ and γ forms of DnaX are required for Caulobacter viability, and identifies a role for clamp loader diversity in responding to DNA damage. The conservation of distinct DnaX isoforms throughout bacteria despite fundamentally different mechanisms for producing them suggests there may be a conserved need for alternate clamp loader complexes during DNA damaging conditions.

  16. The Response Clamp: Functional characterization of neural systems using closed-loop control

    Avner eWallach


    Full Text Available The voltage clamp method, pioneered by Hodgkin, Huxley and Katz, laid the foundations to neurophysiological research. Its core rationale is the use of closed-loop control as a tool for system characterization. A recently introduced method, the response clamp, extends the voltage clamp rationale to the functional, phenomenological level. The method consists of on-line estimation of a response variable of interest (e.g. the probability of response or its latency and a simple feedback control mechanism designed to tightly converge this variable towards a desired trajectory. In the present contribution I offer a perspective on this novel method and its applications in the broader context of system identification and characterization. First, I demonstrate how internal state variables are exposed using the method, and how the use of several controllers may allow for a detailed, multi-variable characterization of the system. Second, I discuss three different categories of applications of the method: (i exploration of intrinsically generated dynamics, (ii exploration of extrinsically generated dynamics and (iii generation of input-output trajectories. The relation of these categories to similar uses in the voltage clamp and other techniques is also discussed. Finally, I discuss the method’s limitations, as well as its possible synthesis with existing complementary approaches.

  17. Trans-Z-source and Γ-Z-source neutral-point-clamped inverters

    Wei, Mo; Loh, Poh Chiang; Blaabjerg, Frede


    Z-source neutral-point-clamped (NPC) inverters are earlier proposed for obtaining voltage buck-boost and three-level switching simultaneously. Their performances are, however, constrained by a trade-off between their input-to-output gain and modulation ratio. This trade-off can lead to high...

  18. Early vs delayed clamping of the umbilical cord in full term, preterm and very preterm infants

    Moller, N.K.; Weber, T.


    Randomized studies from 2006 and two meta-analyses published in 2007 agree that clamping of the umbilical cord can be delayed. For the preterm and very preterm infant benefits include less need for blood transfusion and less morbidity, especially for the very preterm male infant. For the term...

  19. Thermal and Efficiency Analysis of Five-Level Multilevel-Clamped Multilevel Converter Considering Grid Codes

    Ma, Ke; Muñoz-Aguilar, R. S.; Rodriguez, Pedro;


    In this paper, a five-level multilevel-clamped multilevel converter (5L- MLC2) topology is analyzed based on a typical grid integration application for renewable energy system. The loss and thermal distributions of the power devices in different switching legs are investigated and illustrated und...

  20. Five-Level Active-Neutral-Point-Clamped DC/DC Converter

    Liu, Dong; Deng, Fujin; Chen, Zhe

    Multi-level converters are getting more and more attentions because of their obvious merits such as lower voltage stress and harmonic, smaller size of output filters, and so on. In this paper, a five-level active-neutral-point-clamped (5L-ANPC) dc/dc converter is proposed for power transfer...

  1. Characterization of a patch-clamp microchannel array towards neuronal networks analysis

    Alberti, Massimo; Snakenborg, Detlef; Lopacinska, Joanna M.


    for simultaneous patch clamping of cultured cells or neurons in the same network. A disposable silicon/silicon dioxide (Si/SiO2) chip with a microhole array was integrated in a microfluidic system for cell handling, perfusion and electrical recording. Fluidic characterization showed that our PC mu CA can work...

  2. A bulldog clamp that was forgotten during a coronary artery bypass operation 8 years ago.

    Celkan, Mehmet Adnan; Bayatli, Kivanc


    Prevention of retained foreign bodies in the pericardial cavity is critical for patient safety. We report a patient with a bulldog clamp that was forgotten during a coronary artery bypass operation 8 years ago, which was detected by chance and removed.

  3. Glucose consumption by various tissues in pregnant rats : effects of a 6-day euglycaemic hyperinsulinaemic clamp

    Nieuwenhuizen, AG; Schuiling, GA; Bonen, A; Paans, AMJ; Vaalburg, W; Koiter, TR


    In the course of pregnancy maternal tissues become increasingly more insensitive to insulin. As 6 days of euglycaemic hyperinsulinaemic clamping, from day 8 until 14 of gestation, ameliorates total glucose consumption, we analysed the contribution of individual tissues in this phenomenon. We measure

  4. Experimental and numerical analysis of clamped joints in front motorbike suspensions

    Vincenzi N.


    Full Text Available Clamped joints are shaft-hub connections used, as an instance, in front motorbike suspensions to lock the steering plates with the legs and the legs with the wheel pin, by means of one or two bolts. The preloading force, produced during the tightening process, should be evaluated accurately, since it must lock safely the shaft, without overcoming the yielding point of the hub. Firstly, friction coefficients have been evaluated on “ad-hoc designed” specimens, by applying the Design of Experiment approach: the applied tightening torque has been precisely related to the imposed preloading force. Then, the tensile state of clamps have been evaluated both via FEM and by leveraging some design formulae proposed by the Authors as function of the preloading force and of the clamp geometry. Finally, the results have been compared to those given by some strain gauges applied on the tested clamps: the discrepancies between numerical analyses, the design formulae and the experimental results remains under a threshold of 10%.

  5. 井下智能节流器的设计与节流阀流场分析%Design and Flow Field Analysis of Downhole Intelligent Throttle

    何明格; 马发明; 林丽君; 殷国富


    In light of the needs of downhole intelligent throttling technological development,a set of downhole intelligent throttle suitable for remote control was designed.The structure and control mechanism of the intelligent throttling were introduced.The structural design and analytical method of the throttle were expounded.The ANSYS finite element software was applied to make a mechanical analysis of the throttle structure.The flow field analysis method was used to analyze the pressure,velocity and temperature field of the fluid in the key positions of the throttle.The analysis shows that the designed throttle structure satisfies the requirement.It can provide the equipment support for the implementation of downhole intelligent throttling technology.And it can serve as a reference for the development of relevant intelligent downhole tools.%为了满足智能完井和数字化油气田技术的发展需要,设计了一套适用于远程控制的井下智能节流器.介绍了智能节流器的结构体系和控制机理.通过ANSYS有限元软件对井下智能节流器结构进行了力学分析.采用流场分析方法对节流器的关键位置处流体的压力、速度以及温度场进行了分析.分析结果表明,设计的节流器结构满足要求,能为井下智能节流工艺技术的实施提供设备支撑,对相关智能化井下工具的研发具有参考意义.

  6. Application of Downhole Magnetic Field Measurements in the Identification of Petrological Variations in Basalts, Gabbros and Volcaniclastic Sediments

    Bartetzko, A.; Heike, D.


    Downhole magnetic field measurements are routinely carried out during many downhole-logging operations for spatial orientation of borehole wall images. The tools used for this purpose, like the Schlumberger General Purpose Inclinometer Tool (GPIT), were not specifically developed for geological interpretations but comparisons with measurements from precise magnetometers show very good correlations. However, systematic value shifts sometimes occur in some holes and this means that data from the GPIT should be used only qualitatively. We show examples from several holes drilled by the ODP demonstrating the potential of magnetic field logs for geologic and petrologic purposes. Variations in the magnetic field data are caused by different geologic processes in these examples. Injections of Fe-Ti-oxide rich gabbros into olivine gabbro of the lower oceanic crust drilled in ODP Holes 735B and 1105A (SW Indian Ridge) cause distinct signals in the magnetic field logs. The vertical resolution of the tool allows detection of thin layers (10 cm minimum thickness) with small anomalies in the magnetic field logs. Cyclicity in eruption processes at mid-ocean ridges can be revealed using the magnetic field logs. Slight petrologic differences between magmas from different eruptions and changes in the Earth?s magnetic field due to reversals, or secular variations in pauses between the eruptions cause characteristic patterns in the logs (e.g. ODP Holes 395A and 418A). Cooling and subsequent alteration processes cause the formation of different types of Fe- and/or Ti-oxide minerals. Typical examples of the formation of secondary magnetic minerals in subaerial lava flows are seen in ODP Hole 1137A (Kerguelen Plateau). Characteristic anomalies in the magnetic field log correlate well with total gamma ray measurement, which is an indicator for alteration in this type of rocks. Grain Size linked with crystallinity variations in basaltic volcaniclastic deposits and debris flows influence

  7. Age depth-model for the past 630 ka in Lake Ohrid (Macedonia/Albania based on cyclostratigraphic analysis of downhole gamma ray data

    H. Baumgarten


    Full Text Available We report the gamma ray fluctuations from downhole logging data obtained in the sediments of Lake Ohrid from 0 to 240 m below lake floor. These variations in gamma ray and potassium values strongly correlate with fluctuations in global δ18O values and can be thus considered a reliable proxy to depict glacial–interglacial cycles, with high clastic input during cold and/or drier periods and high carbonate precipitation during the warm and/or humid periods in Lake Ohrid. Spectral analysis (sliding window was applied to investigate the climate signal and their evolution over the length of the borehole. Linking the downhole logging data with orbital cycles was used to estimate sedimentation rates, which shift from 45 cm ka−1 between 0 to 110 m to 30 cm ka−1 from 110 to 240 m below lake floor. The effect of compaction was compensated for. Sedimentation rates increase on average by 14% after decompaction of the sediment layers. Tuning of minima and maxima in gamma ray and potassium values vs. LR04 minima and maxima, in combination with eight independent tephrostratigraphical tie points, allows the establishment of a robust age model for the downhole logging data over the past 630 ka in Lake Ohrid.

  8. Numerical simulation of downhole temperature distribution in producing oil wells%生产油井井下温度场数值模拟分析

    石颖; 宋延杰; 刘洪


    An improved numerical simulation method is presented to calculate the downhole temperature distribution for multiple pay zones in producing oil wells. Based on hydrodynamics and heat transfer theory, a 2-D temperature field model in cylindrical coordinates is developed. In the model, we considered general heat conduction as well as the heat convection due to fluid flow from porous formation to the borehole. We also take into account the fluid velocity variation in the wellbore due to multiple pay zones. We present coupled boundary conditions at the interfaces between the wellbore and adjacent formation, the wellbore and pay zone, and the pay zone and adjacent formation. Finally, an alternating direction implicit difference method (ADI) is used to solve the temperature model for the downhole temperature distribution. The comparison of modeled temperature curve with actual temperature log indicates that simulation result is in general quite similar to the actual temperature log. We found that the total production rate, production time, porosity, thickness of pay zones, and geothermal gradient, all have effects on the downhole temperature distribution.

  9. 井下作业铅模打印技术研究与分析%Analysis on the technology of downhole operation lead model print

    刘成贵; 蒋金平; 王海龙; 黄丽; 杨景堂


    During the construction of downhole operation, playing lead mold is an important way to understand the downhole casing fish top form and condition. This paper elaborated the technical parameters and lead mold analysis steps. The author analyzed and sorted many lead mold into the downhole, formed the analysis method for poles fall, tube fall, rope falling objects, technical conditions of conductor pipe and the other technical condition, and formulate the corresponding treatment measures according to different impressions.%井下作业施工,打铅印是了解井下落物鱼顶形态和套管状况的重要方法。本文对打印的技术参数和印模分析步骤进行了阐述,对多年入井的铅印进行了分析、整理和归纳,形成了针对杆类落物、管类落物、绳类落物、套管技术状况以及井下其它技术状况的印模分析方法,并制定了针对不同印痕的对应处理措施。

  10. An AFM-based stiffness clamp for dynamic control of rigidity.

    Webster, Kevin D; Crow, Ailey; Fletcher, Daniel A


    Atomic force microscopy (AFM) has become a powerful tool for measuring material properties in biology and imposing mechanical boundary conditions on samples from single molecules to cells and tissues. Constant force or constant height can be maintained in an AFM experiment through feedback control of cantilever deflection, known respectively as a 'force clamp' or 'position clamp'. However, stiffness, the third variable in the Hookean relation F = kx that describes AFM cantilever deflection, has not been dynamically controllable in the same way. Here we present and demonstrate a 'stiffness clamp' that can vary the apparent stiffness of an AFM cantilever. This method, employable on any AFM system by modifying feedback control of the cantilever, allows rapid and reversible tuning of the stiffness exposed to the sample in a way that can decouple the role of stiffness from force and deformation. We demonstrated the AFM stiffness clamp on two different samples: a contracting fibroblast cell and an expanding polyacrylamide hydrogel. We found that the fibroblast, a cell type that secretes and organizes the extracellular matrix, exhibited a rapid, sub-second change in traction rate (dF/dt) and contraction velocity (dx/dt) in response to step changes in stiffness between 1-100 nN/µm. This response was independent of the absolute contractile force and cell height, demonstrating that cells can react directly to changes in stiffness alone. In contrast, the hydrogel used in our experiment maintained a constant expansion velocity (dx/dt) over this range of stiffness, while the traction rate (dF/dt) changed with stiffness, showing that passive materials can also behave differently in different stiffness environments. The AFM stiffness clamp presented here, which is applicable to mechanical measurements on both biological and non-biological samples, may be used to investigate cellular mechanotransduction under a wide range of controlled mechanical boundary conditions.

  11. Ventilation before Umbilical Cord Clamping improves the physiological transition at birth.

    Sasmira eBhatt


    Full Text Available The transition from a fetus to a neonate at birth represents a critical phase in our life. Most infants make this transition without complications, but preterm infants usually require some form of assistance due to immature cardiopulmonary systems that predispose them to lifelong sequelae. As the incidence of preterm birth is increasing, there is now an urgent need for the development of management strategies that facilitate this transition, which will likely include improved strategies for the management of the maternal third stage of labour. For instance, recent studies on the physiological transition at birth have led to the discovery that establishing ventilation in the infant before the umbilical cord is clamped greatly stabilizes the cardiovascular transition at birth. While most benefits of delayed clamping have previously been attributed to an increase in placenta to infant blood transfusion, clearly there are other significant benefits for the infant, which are not well understood. Nevertheless, if ventilation can be established before cord clamping in a preterm infant, the large adverse changes in cardiac function that normally accompanies umbilical cord clamping can be avoided. As preterm infants have an immature cerebral vascular bed, large swings in cardiovascular function places them at high risk of cerebral vascular rupture and the associated increased risk of mortality and morbidity. In view of the impact that cord clamping has on the cardiovascular transition at birth, it is also time to re-examine some of the strategies used in the management of the third stage of labour. These include the appropriate timing of uterotonic administration in relation to delivery of the infant and placenta. As there is a lack of evidence on the effects these individual practices have on the infant, there is a necessity to improve our understanding of their impact in order to develop strategies that facilitate the transition to newborn life.

  12. Timing of Events in the Central Engine and Jets of the Radio Galaxies 3c 111 and 3c 120 (core Program)

    The investigators request continuation of their long-term monitoring of the X-ray flux of the radio galaxies 3C 111 (FR 2) and 3C 120 (FR 1) 2 and 4 times per week, respectively, throughout Cycle 12, as well as a 90 days of daily monitoring of 3C 111. In both objects, dips in X-ray flux precede the appearance of bright superluminal knots in the radio jet. The long-term multiwaveband light curves and sequences of 7 mm VLBA images will record the changing pattern of multiwaveband emission in these two AGN. 3C 111 is a probable EGRET source; if the ID is correct, GLAST will measure its flux daily, allowing relative timing of gamma-ray variations with X-ray and optical events from the central engine plus radio events in the jet.

  13. Evaluation of diel patterns of relative changes in cell turgor of tomato plants using leaf patch clamp pressure probes

    Lee, K.M.; Driever, S.M.; Heuvelink, E.; Rüger, S.; Zimmermann, U.; Gelder, de A.; Marcelis, L.F.M.


    Relative changes in cell turgor of leaves of well-watered tomato plants were evaluated using the leaf patch clamp pressure probe (LPCP) under dynamic greenhouse climate conditions. Leaf patch clamp pressure changes, a measure for relative changes in cell turgor, were monitored at three different hei

  14. MATLAB implementation of a dynamic clamp with bandwidth >125 KHz capable of generating INa at 37°C

    Clausen, Chris; Valiunas, Virginijus; Brink, Peter R.; Cohen, Ira S.


    We describe the construction of a dynamic clamp with bandwidth >125 KHz that utilizes a high performance, yet low cost, standard home/office PC interfaced with a high-speed (16 bit) data acquisition module. High bandwidth is achieved by exploiting recently available software advances (code-generation technology, optimized real-time kernel). Dynamic-clamp programs are constructed using Simulink, a visual programming language. Blocks for computation of membrane currents are written in the high-level matlab language; no programming in C is required. The instrument can be used in single- or dual-cell configurations, with the capability to modify programs while experiments are in progress. We describe an algorithm for computing the fast transient Na+ current (INa) in real time, and test its accuracy and stability using rate constants appropriate for 37°C. We then construct a program capable of supplying three currents to a cell preparation: INa, the hyperpolarizing-activated inward pacemaker current (If), and an inward-rectifier K+ current (IK1). The program corrects for the IR drop due to electrode current flow, and also records all voltages and currents. We tested this program on dual patch-clamped HEK293 cells where the dynamic clamp controls a current-clamp amplifier and a voltage-clamp amplifier controls membrane potential, and current-clamped HEK293 cells where the dynamic clamp produces spontaneous pacing behavior exhibiting Na+ spikes in otherwise passive cells. PMID:23224681

  15. Synthesis and biological evaluation of branched and conformationally restricted analogs of the anticancer compounds 3'-C-ethynyluridine (EUrd) and 3'-C-ethynylcytidine (ECyd)

    Hrdlicka, Patrick J; Andersen, Nicolai K; Jepsen, Jan S;


    The synthesis of branched and conformationally restricted analogs of the anticancer nucleosides 3'-C-ethynyluridine (EUrd) and 3'-C-ethynylcytidine (ECyd) is presented. Molecular modeling and (1)H NMR coupling constant analysis revealed that the furanose rings of all analogs except the LNA analog...... are conformationally biased towards South conformation, and are thus mimicking the structure of ECyd. All target nucleosides were devoid of anti-HIV or anticancer activity....

  16. Roles of the Picornaviral 3C Proteinase in the Viral Life Cycle and Host Cells.

    Sun, Di; Chen, Shun; Cheng, Anchun; Wang, Mingshu


    The Picornaviridae family comprises a large group of non-enveloped viruses that have a major impact on human and veterinary health. The viral genome contains one open reading frame encoding a single polyprotein that can be processed by viral proteinases. The crucial 3C proteinases (3C(pro)s) of picornaviruses share similar spatial structures and it is becoming apparent that 3C(pro) plays a significant role in the viral life cycle and virus host interaction. Importantly, the proteinase and RNA-binding activity of 3C(pro) are involved in viral polyprotein processing and the initiation of viral RNA synthesis. In addition, 3C(pro) can induce the cleavage of certain cellular factors required for transcription, translation and nucleocytoplasmic trafficking to modulate cell physiology for viral replication. Due to interactions between 3C(pro) and these essential factors, 3C(pro) is also involved in viral pathogenesis to support efficient infection. Furthermore, based on the structural conservation, the development of irreversible inhibitors and discovery of non-covalent inhibitors for 3C(pro) are ongoing and a better understanding of the roles played by 3C(pro) may provide insights into the development of potential antiviral treatments. In this review, the current knowledge regarding the structural features, multiple functions in the viral life cycle, pathogen host interaction, and development of antiviral compounds for 3C(pro) is summarized.

  17. Powders with superparamagnetic Fe{sub 3}C particles studied with Moessbauer spectrometry

    David, B; Schneeweiss, O [Institute of Physics of Materials, Academy of Sciences of the Czech Republic, v.v.i., Ziz' kova 22, CZ-61662 Brno (Czech Republic); Dumitrache, F; Fleaca, C; Alexandrescu, R; Morjan, I [National Institute for Lasers, Plasma and Radiation Physics, PO Box MG-36, 077125 Bucharest-Magurele (Romania)


    Two nanopowders with superparamagnetic Fe{sub 3}C particles were synthesised by the method of laser-induced pyrolysis of gaseous precursors. Both were characterised by X-ray diffraction, Moessbauer spectrometry and standard magnetic measurements. The mean crystallite size of Fe{sub 3}C was 3 nm for the first sample and 10 nm for the second sample (Scherrer formula), i.e. it was lower than in our previously studied ferromagnetic Fe{sub 3}C-based sample. Fe{sub 3}C phase in both present samples exhibited by {approx}20 K reduced Curie temperature which is interpreted as a nanosize effect. After annealing of the samples at 1073 K for 30 minutes the Curie temperature of the Fe{sub 3}C phase in both samples matched its standard bulk value. Beside Fe{sub 3}C phase also Fe{sub 3}O{sub 4} and carbon black were present in the synthesised samples.

  18. Powders with superparamagnetic Fe3C particles studied with Mössbauer spectrometry

    David, B.; Schneeweiss, O.; Dumitrache, F.; Fleaca, C.; Alexandrescu, R.; Morjan, I.


    Two nanopowders with superparamagnetic Fe3C particles were synthesised by the method of laser-induced pyrolysis of gaseous precursors. Both were characterised by X-ray diffraction, Mössbauer spectrometry and standard magnetic measurements. The mean crystallite size of Fe3C was 3 nm for the first sample and 10 nm for the second sample (Scherrer formula), i.e. it was lower than in our previously studied ferromagnetic Fe3C-based sample. Fe3C phase in both present samples exhibited by ~20 K reduced Curie temperature which is interpreted as a nanosize effect. After annealing of the samples at 1073 K for 30 minutes the Curie temperature of the Fe3C phase in both samples matched its standard bulk value. Beside Fe3C phase also Fe3O4 and carbon black were present in the synthesised samples.

  19. Induction of DNA DSB and its rejoining in clamped and non-clamped tumours after exposure to carbon ion beams in comparison to X rays.

    Hirayama, R; Uzawa, A; Matsumoto, Y; Noguchi, M; Kase, Y; Takase, N; Ito, A; Koike, S; Ando, K; Okayasu, R; Furusawa, Y


    We studied double-strand breaks (DSB) induction and rejoining in clamped and non-clamped transplanted tumours in mice leg after exposure to 80 keV µm(-1) carbon ions and X rays. The yields of DSB in the tumours were analysed by a static-field gel electrophoresis. The OER of DSB after X rays was 1.68±0.31, and this value was not changed after 1 h rejoining time (1.40±0.26). These damages in oxygenated conditions were rejoined 60-70% within 1 h in situ. No difference was found between the exposure to X rays and carbon ions for the induction and rejoining of DSB. Thus, the values of OER and rejoined fraction after exposure to carbon ions were similar to those after X rays, and the calculated relative biological effectivenesses of carbon ion were around 1 under both oxygen conditions. The yields of DSB in vivo depend on exposure doses, oxygen conditions and rejoining time, but not on the types of radiation quality.

  20. Detailed Theoretical Model for Adjustable Gain-Clamped Semiconductor Optical Amplifier

    Lin Liu


    Full Text Available The adjustable gain-clamped semiconductor optical amplifier (AGC-SOA uses two SOAs in a ring-cavity topology: one to amplify the signal and the other to control the gain. The device was designed to maximize the output saturated power while adjusting gain to regulate power differences between packets without loss of linearity. This type of subsystem can be used for power equalisation and linear amplification in packet-based dynamic systems such as passive optical networks (PONs. A detailed theoretical model is presented in this paper to simulate the operation of the AGC-SOA, which gives a better understanding of the underlying gain clamping mechanics. Simulations and comparisons with steady-state and dynamic gain modulation experimental performance are given which validate the model.

  1. Implementation and Tuning of an Optical Tweezers Force-Clamp Feedback System.

    Bugiel, Michael; Jannasch, Anita; Schäffer, Erik


    Feedback systems can be used to control the value of a system variable. In optical tweezers, active feedback is often implemented to either keep the position or tension applied to a single biomolecule constant. Here, we describe the implementation of the latter: an optical force-clamp setup that can be used to study the motion of processive molecular motors under a constant load. We describe the basics of a software-implemented proportional-integral-derivative (PID) controller, how to tune it, and how to determine its optimal feedback rate. Limitations, possible feed-forward applications, and extensions into two- and three-dimensional optical force clamps are discussed. The feedback is ultimately limited by thermal fluctuations and the compliance of the involved molecules. To investigate a particular mechanical process, understanding the basics and limitations of the feedback system will be helpful for choosing the proper feedback hardware, for optimizing the system parameters, and for the design of the experiment.

  2. Influence of non-steady state during isoglycemic hyperinsulinemic clamp in hypertension. A LIFE substudy

    Olsen, M H; Andersen, U B; Wachtell, K;


    We wanted to investigate whether time to steady state was reached within 2 h of insulin infusion during isoglycemic hyperinsulinemic clamp, comparing the glucose uptake index (M/IG) with Bergman's insulin sensitivity index (Sip). We performed a 2-h oral glucose tolerance test and a 3-h isoglycemic...... hyperinsulinemic clamp in 26 young, healthy subjects and 43 elderly patients with unmedicated essential hypertension and left ventricular hypertrophy. The 3-h Sip correlated strongly with the 2-h M/IG in the patients (r = 0.88, p ... narrow limits of agreement in the patients. However, during the third hour of insulin infusion, M/IG (10.0 vs 12.21(2) x kg(-1) x min(-1) x mmol(-1), p Sip (7.1 vs 9.41(2) x kg(-1) x min(-1) x mmol(-1), p

  3. [Traumatic section of the thoracic aorta: its repair by direct aortic clamping].

    Cairols, M A; Sieyro, F; Miralles, M; Blanes, I; Lozano, P


    Traumatic section of descendent aorta, severe complication of a thoracic traumatism, requires an early recognition and restoration because its high mortality rate. Between 1988 and 1990, three patients underwent surgical approach, by direct aortic clamping [correction of clamplaje], at our Service. In two cases, a heart stoppage appeared during the clamping/unclamping [correction of clampaje/desclampaje] maneuvers. One of them had previously electrocardiographic abnormalities, suggestive of heart contusion. Two patients died, one of them during surgical procedure because an irreversible heart stoppage, and the second patient died after 7 days because of a brain hemorrhage. One case presented postoperative paraplegia. Respective rates and literature about the main factors implicated in the diagnosis and treatment of such pathology are reviewed.

  4. Study of transmembrane La3+ movement in rat ventricular myocytes by the patch-clamp technique


    We have studied transmembrane La3+ movement in rat ventricular myocytes for the first time by using the whole-cell patch-clamp recording mode. La3+ (0.01-5.0 mmol/L) could not bring out inward currents through the L-type calcium channel in rat ventricular myocytes, while it could enter the cells by the same way carried by 1μmol/L ionomycin. When the outward Na+ concentration gradient is formed, La3+ can enter the cells via Na-Ca exchange, and the exchange currentsincrease with the increase of external La3+ concentrations. But compared with Na-Ca exchange currents in the same concentration, the former is only 14%-38% of the latter. The patch-clamp experiment indicates that La3+ normally can not enter ventricular myocytes through L-type calcium channel, but it can enter the cells via Na-Ca exchange.

  5. High Efficiency Interleaved Active Clamped Dc-Dc Converter with Fuel Cell for High Voltage Applications

    Sona P


    Full Text Available A high efficiency interleaved ZVS active clamped current fed dc-dc converter is proposed in this paper specially used for fuel cell applications. As the fuel cell output is very low we are in need of a step up dc-dc converter. Here a current fed dc-dc converter is used. Two current fed dc-dc converters are interleaved by connecting their inputs in parallel and outputs in series. With this proposed methodology input current ripples in the fuel cell stacks can be reduced and a regulated output voltage ripples can be obtained. The active clamping circuit used in this model absorbs the turn off voltage spikes hence low voltage devices with low on state resistance can be used.Voltage doubler circuits will give double the output voltage than normal with smaller transformer turns ratio and flexibility. The proposed method is simulated in MATLAB for verifying the accuracy of the proposed design.

  6. Short Hypoxia Does not Affect Plasma Leptin in Healthy Men under Euglycemic Clamp Conditions

    Andre Schmoller


    Full Text Available Leptin is involved in the endocrine control of energy expenditure and body weight regulation. Previous studies emphasize a relationship between hypoxic states and leptin concentrations. The aim of this study was to investigate the effects of acute hypoxia on leptin concentrations in healthy subjects. We examined 14 healthy men. Hypoxic conditions were induced by decreasing oxygen saturation to 75% for 30 minutes. Plasma leptin concentrations were determined at baseline, after 3 hours of euglycemic clamping, during hypoxia, and repeatedly the following 2.5 hours thereafter. Our results show an increase of plasma leptin concentrations in the course of 6 hours of hyperinsulinemic-euglycemic clamping which may reflect diurnal rhythmicity. Notwithstanding, there was no difference between levels of leptin in the hypoxic and the normoxic condition (=.2. Since we did not find any significant changes in leptin responses upon hypoxia, plasma leptin levels do not seem to be affected by short hypoxic episodes of moderate degree.

  7. Testing and evaluation of stretching strain in clamped–clamped beams for energy harvesting

    Emad, Ahmed; Mahmoud, Mohamed A. E.; Ghoneima, Maged; Dessouky, Mohamed


    In this paper, evaluation of stretching strain capabilities to harvest energy from a piezoelectric clamped–clamped beam is theoretically modeled and experimentally tested. The utilization of stretching strain has many advantages as: elimination of any substrate material, and the simple electrode configuration. The doubly clamped structure exhibits a highly nonlinear frequency response (Hardening Duffing) that widens the bandwidth during the frequency up-ward sweep. The wide bandwidth makes it suitable for practical applications. A design of 53.5 {{mm}}3 (29.7 {{mm}}3 piezoelectric material + 23.8 {{mm}}3 proof mass) energy harvester was tested using PVDF (polyvinylidene fluoride) that can generate up to 15 μW from vibrations of 0.5{g} at 128 {Hz} and 2 MΩ load. The design can also generate up to 41 μ {{W}} from vibrations of 1{g} at 140 {Hz} and 2 MΩ load.

  8. Conformal analysis of fundamental frequency of vibration of elastic clamped plates

    QI Hongyuan; ZHU Hengjun


    To calculate the fundamental frequency of vibration of special-shaped and elastic clamped plates, the conformal mapping theory is adopted to separate the interpolating points of a complicated boundary into odd and even sequences,both of which can be mutually iterated, so that the conformal mapping function between the complicated region and the unit dish region is established. Trigonometric interpolation and convergence along the normal direction methods are provided, and the complex coefficients of the conformal mapping function are calculated. Galerkin method is used to obtain the solution of fundamental frequency in the vibrating differential function of the complicated vibrating region.Finally, taking ellipse elastic clamped plates as an example,the effects on fundamental frequency coefficient caused by eccentric ratio e and area size are analyzed.

  9. MATLAB-based automated patch-clamp system for awake behaving mice

    Desai, Niraj S.; Siegel, Jennifer J.; Taylor, William; Chitwood, Raymond A.; Johnston, Daniel


    Automation has been an important part of biomedical research for decades, and the use of automated and robotic systems is now standard for such tasks as DNA sequencing, microfluidics, and high-throughput screening. Recently, Kodandaramaiah and colleagues (Nat Methods 9: 585–587, 2012) demonstrated, using anesthetized animals, the feasibility of automating blind patch-clamp recordings in vivo. Blind patch is a good target for automation because it is a complex yet highly stereotyped process th...

  10. Microwave electromechanical resonator consisting of clamped carbon nanotubes in an abacus arrangement

    Peng, H. B.; Chang, C. W.; Aloni, S.; Yuzvinsky, T. D.; Zettl, A.


    We describe nanoscale electromechanical resonators capable of operating in ambient-pressure air at room temperature with unprecedented fundamental resonance frequency of ˜4GHz . The devices are created from suspended carbon nanotubes loaded abacus style with inertial metal clamps, yielding short effective beam lengths. We examine the energy dissipation in the system due to air damping and contact loss. Such nanoabacus resonators open windows for immediate practical microwave frequency nanoelectromechanical system applications.

  11. Generation of high-performance binding proteins for peptide motifs by affinity clamping

    Koide, Shohei; Huang, Jin


    We describe concepts and methodologies for generating “Affinity Clamps”, a new class of recombinant binding proteins that achieve high affinity and high specificity toward short peptide motifs of biological importance, which is a major challenge in protein engineering. The Affinity Clamping concept exploits the potential of nonhomologous recombination of protein domains in generating large changes in protein function and the inherent binding affinity and specificity of the so-called modular i...

  12. Pulse-Width-Modulation of Neutral-Point-Clamped Sparse Matrix Converter

    Loh, P.C.; Blaabjerg, Frede; Gao, F.


    literature. Addressing the afore-described issues, this paper focuses on the operational mode analysis of a three-level sparse matrix converter implemented using a neutral-point-clamped inversion stage, and  the design of a number of PWM and modulation ratio compensation schemes for controlling the converter...... with improved waveform quality. Performances and practicalities of the designed schemes are verified in simulation and experimentally using an implemented laboratory prototype with some representative results captured and presented in the paper....

  13. Adverse impact of intermittent portal clamping on long-term postoperative outcomes in hepatocellular carcinoma.

    Hao, S; Chen, S; Yang, X; Wan, C


    Introduction To evaluate the impact of intermittent portal clamping (IPC) on long-term postoperative outcomes in patients with hepatocellular carcinoma (HCC). Methods Clinical records of 355 patients underwent curative liver resection for HCC in January 2007 to December 2010 were retrospectively reviewed. According to how portal clamping was performed, patients were grouped as: IPC, n=113; other portal clamping (OPC), n=190; and no portal clamping (NPC), n=52. Results Median recurrence-free survival (RFS) was statistically significantly shorter in the IPC (39.4 months) than OPC (47.3 months, p=0.010) and NPC groups (51.4 months, p=0.008). Median overall survival (OS) was also significantly shorter with IPC (46.3 months), versus 52.9 months with OPC (p=0.022) and 56.2 months with NPC (p=0.015). Kaplan-Meier survival analysis revealed that 5-year cumulative RFS was much lower in the IPC (42.5%) than OPC (50.9%, p=0.014) and NPC groups (49.6%, p=0.013). Five-year cumulative OS was also much lower in the IPC (44.9%) than OPC (58.0%, p=0.020) and NPC groups (57.7%, p=0.025). On univariate analysis, tumour grade, size and number, TNM stage, blood transfusion, vascular invasion and IPC were significantly inversely correlated with RFS and OS. On multivariate analysis, tumour size and number, blood transfusion, vascular invasion and IPC remained significant. Conclusions Our study suggests that IPC is an independent risk factor for poor long-term postoperative outcomes in patients with HCC.

  14. Prediction of numerical distortion after welding with various welding sequences and clampings

    S. Kastelic


    Full Text Available Welding simulation of a test cover for hydropower plant was made due to very large dimensions of the cover. The main aim was to predict distortion after welding in order to avoid machining the cover. Welding process was simulated with the Sysweld program to keep distortion in desired limits. Various welding sequences and clamping conditions were calculated to reduce the distortion. Calculation of microstructure constituents in virtual complex geometry of joints was also analyzed.

  15. Direct-conversion switching-mode audio power amplifier with active capacitive voltage clamp

    Ljusev, Petar; Andersen, Michael Andreas E.


    This paper discusses the advantages and problems when implementing direct energy conversion switching-mode audio power amplifiers. It is shown that the total integration of the power supply and Class D audio power amplifier into one compact direct converter can simplify the design, increase...... efficiency, reduce the product volume and lower its cost. As an example, the principle of operation and the measurements made on a direct-conversion switching-mode audio power amplifier with active capacitive voltage clamp are presented....

  16. New device for saphenous vein-to-aorta proximal anastomosis without side-clamping

    Tappainer Ernesto


    Full Text Available Abstract Background Side clamping to perform saphenous vein-to-aorta proximal anastomosis is a well known cause of cerebral embolization during coronary bypass surgery. Automatic and manual devices have been introduced to avoid aortic clamping and facilitate proximal anastomosis but the manual ones only allow the traditional hand-sewing running suture. Nevertheless, they are not easy to use and very expensive to buy. Methods We developed a simple object that helps to perform a manual proximal anastomosis without the need to clamp the side of the aorta. This device is a steel bar which blocks the aortic hole and simultaneously it provides a slit to receive the needle. Through the slit comes out a thin, sharp, straight, but also well directed and predictable jet of blood which could be easily controlled during the suture. Results The function of the object is quite different from other devices. Nothing is deployed in the aorta. The object is only placed on the aorta with the small appendage slipped into the hole. The main advantage of the device is that while manipulation of the aorta is avoided no foreign bodies are incorporated in the suture and – most importantly – the aortic intima is not touched at all. The main drawback of the device is the blood jet coming from the slit so that the blood pressure has to be lowered by vasodilators during the anastomosis. Moreover, the suture has to change direction and the needle has to enter the aortic wall first to slip out through the slit. Conclusion The object was named "Slit Device" and is not a routine instrument. It would be only an alternative to other anastomotic devices with the same surgical indications. In the case of ascending aortic disease and saphenous vein grafting, the Slit Device avoids aortic clamping thereby preventing atheroembolism and also avoiding the need for hypothermic circulatory arrest in patients with unclampable aorta.

  17. A complete dc characterization of a constant-frequency, clamped-mode, series-resonant converter

    Tsai, Fu-Sheng; Lee, Fred C.


    The dc behavior of a clamped-mode series-resonant converter is characterized systematically. Given a circuit operating condition, the converter's mode of operation is determined and various circuit parameters are calculated, such as average inductor current (load current), rms inductor current, peak capacitor voltage, rms switch currents, average diode currents, switch turn-on currents, and switch turn-off currents. Regions of operation are defined, and various circuit characteristics are derived to facilitate the converter design.

  18. An ANN Based Capicitor Voltage Balancing Method For Neutral Point Clamped Multi-Level Inverter

    P.J.S. Praveen,


    Full Text Available Multi-level inverters are became popular for usage in medium voltage, low voltage power applications due to flexibility in control and better performance characteristics in terms of harmonic regulation. Neutral point clamped are popular as they require less number of sources as their input when compared with their counter parts i.e. cascaded multi-level inverters and found to be reliable when compared with flying capacitor based multi-level inverters. But when neutral clamped technologies are used for generation of three-phase voltages, the capacitors that are connected at input side experiences imbalance in their voltages, this makes neutral point clamped multi-level inverters less reliable. In the proposed work an attempt is made to study circuits that balances the capacitor voltages and a scheme is investigated for balancing the capacitor voltages. Method proposed in [1] uses PID controller for balancing the capacitor voltages. In this project PI based control scheme and artificial neural network (ANN based control scheme for the front end circuit shown in [1] are designed for achieving balance among the capacitor voltages. The proposed control scheme is simulated with the help of Simpowersystems block set and neural network toolbox of MATLAB software for different load conditions. Results obtained from ANN based controller and PI controller are presented.

  19. Low noise patch-clamp current amplification by nanoparticles plasmonic-photonic coupling (analysis and modelling).

    Haberal, E O; SalmanOgli, A; Nasseri, B


    In this article, a patch-clamp low noise current amplification based on nanoparticles plasmonic radiation is analyzed. It is well-known, a very small current is flowing from different membrane channels and so, for extra processing the current amplification is necessary. It is notable that there are some problems in traditional electronic amplifier due to its noise and bandwidth problem. Because of the important role of the patch-clamp current in cancer research and especially its small amplitude, it is vital to intensify it without adding any noises. In this study, the current amplification is performed firstly: from the excitement of nanoparticles by the patch-clamp pico-ampere current and then, the effect of nanoparticles plasmonic far-field radiation on conductor's carriers, which will cause the current amplification. This relates to the plasmonic-photonic coupling and their effect on conductor carriers as the current perturbation agent. In the steady state, the current amplification can reach to 1000 times of initial level. Furthermore, we investigated the nanoparticles morphology changing effect such as size, nanoparticles inter-distance, and nanoparticles distance from the conductor on the amplifier parameters. Finally, it should note that the original aim is to use nanoparticles plasmonic engineering and their coupling to photonics for output current manipulating.

  20. Desktop Software for Patch-Clamp Raw Binary Data Conversion and Preprocessing

    Ning Zhang


    Full Text Available Since raw data recorded by patch-clamp systems are always stored in binary format, electrophysiologists may experience difficulties with patch clamp data preprocessing especially when they want to analyze by custom-designed algorithms. In this study, we present desktop software, called PCDReader, which could be an effective and convenient solution for patch clamp data preprocessing for daily laboratory use. We designed a novel class module, called clsPulseData, to directly read the raw data along with the parameters recorded from HEKA instruments without any other program support. By a graphical user interface, raw binary data files can be converted into several kinds of ASCII text files for further analysis, with several preprocessing options. And the parameters can also be viewed, modified and exported into ASCII files by a user-friendly Explorer style window. The real-time data loading technique and optimized memory management programming makes PCDReader a fast and efficient tool. The compiled software along with the source code of the clsPulseData class module is freely available to academic and nonprofit users.

  1. Molecular jamming—The cystine slipknot mechanical clamp in all-atom simulations

    Pepłowski, Łukasz; Sikora, Mateusz; Nowak, Wiesław; Cieplak, Marek


    A recent survey of 17 134 proteins has identified a new class of proteins which are expected to yield stretching induced force peaks in the range of 1 nN. Such high force peaks should be due to forcing of a slip-loop through a cystine ring, i.e., by generating a cystine slipknot. The survey has been performed in a simple coarse grained model. Here, we perform all-atom steered molecular dynamics simulations on 15 cystine knot proteins and determine their resistance to stretching. In agreement with previous studies within a coarse grained structure based model, the level of resistance is found to be substantially higher than in proteins in which the mechanical clamp operates through shear. The large stretching forces arise through formation of the cystine slipknot mechanical clamp and the resulting steric jamming. We elucidate the workings of such a clamp in an atomic detail. We also study the behavior of five top strength proteins with the shear-based mechanostability in which no jamming is involved. We show that in the atomic model, the jamming state is relieved by moving one amino acid at a time and there is a choice in the selection of the amino acid that advances the first. In contrast, the coarse grained model also allows for a simultaneous passage of two amino acids.

  2. Three Level Diode Clamped Inverter Fed Induction Motor with Dtc-Svm

    V. Ranjith Kumar


    Full Text Available This project presents a three-level diode-clamped inverter feeding an asynchronous motor drive with direct torque control (DTC. The control method is based on DTC operating principles. The stator voltage vector reference is computed from the stator flux and torque errors imposed by the flux and torque controllers. This voltage reference is then generated using a diode-clamped inverter, where each phase of the inverter can be implemented using a dc source, which would be available from fuel cells, batteries, or ultra capacitors. This inverter provides nearly sinusoidal voltages with very low distortion, even without filtering, using fewer switching devices. In addition, the multilevel inverter can generate a high and fixed switching frequency output voltage with fewer switching losses, since only the small power cells of the inverter operate at a high switching rate. Therefore, a high performance and also efficient torque and flux controllers are obtained, enabling a DTC solution for multilevel-inverter-powered motor drives. The Pulse Width modulation technique for an inverter permits to obtain three phase system voltages, which can be applied to the controlled output. Space Vector Modulation (SVPWM principle differs from other PWM processes in the fact that all three drive signals for the inverter will be created simultaneously. The implementation of SVPWM process in digital systems necessitates less operation time and also less program memory. This project uses SVPWM technique for generation of pulses for three-level diode clamped inverter. Simulation is carried out on MATLAB-Simulink software.

  3. A capacitor-clamped inverter based torsional oscillation damping method for electromechanical drivetrains

    Shagar Viknash


    Full Text Available A typical electromechanical drivetrain consists of an electric motor, connecting shafts and gears. Premature failures of these shafts and gears have been reported which are mainly due to fatigue caused by extreme loads and torsional oscillations. Overdesign and passive damping are the common approaches taken to increase the fatigue life. Nevertheless, they increase the system cost, weight and volume. Alternatively, active damping through advanced inverter control of the motor drive has been identified as a promising solution that does not require overdesign or alterations to the existing system. Even with the active damping control, oscillations propagate into the dc side of the power converter and subsequently to the upstream power bus. Generally, a large capacitor or an additional energy storage system is placed to suppress these oscillations. This paper proposes to use the clamping capacitors of the capacitor-clamped inverter as energy storage elements and thereby eliminate the need for a large dc side capacitor or an additional energy storage system. The efficacy of the proposed method has been verified with computer simulations. Simulation results show that the clamping capacitors are capable of containing torsional oscillations within the inverter without passing them to the upstream power bus.

  4. Multifrequency radio observations of 3C 28, 76. 1, 186 and 319

    Macklin, J.T. (Mullard Radio Astronomy Observatory, Cambridge (UK). Cavendish Lab.)


    Multifrequency observations of 3C 28, 76.1, 186 and 319, made with the One-Mile and 5-km telescopes, enable the spectral-index distributions of each source and the polarization properties of 3C 76.1 to be studied. 3C 28 is shown to have the structure of a Fanaroff-Riley class I source, but it is 10 times more luminous than expected for this class. Although 3C 76.1 is also in Fanaroff-Riley class I, it contains a feature which has the properties of a 'hotspot'. The spectral-index distribution in 3C 76.1 is complex; the magnetic field is perpendicular to the axes of the jets, and the polarization properties are consistent with a tangled-field model. The morphology of 3C 186 remains unclear, and 3C 319 is a double source with only one 'hotspot', rather than being a tail source. It is suggested that the different properties of 3C 28 and 76.1 are due to their different environments; this explanation would be consistent with a general scheme which can account for all the observed dependences of source properties on cluster membership.

  5. Retrieve Ocean Bottom and Downhole Seismic sensors orientation using integrated low cost gyroscope and direct rotation measurements

    D'Alessandro, Antonino; D'Anna, Giuseppe


    To reduce the background noise level, seismic sensors are often installed in downhole. During the installation, it is not possible to determine exactly what the sensors has rotated in the horizontal plane before reaching the bottom. To monitoring the seismic activity occurred in offshore areas, Ocean Bottom Seismometers (OBS) are often deployed in the area to be studied. During the OBS descent phase along the seawater column the sensor can undergo to significant rotations in the horizontal plane. Therefore, both for seismic sensors installed in downhole or on ocean bottom, the absolute orientation of the horizontal components are unknown. Clearly, this serious problem can be limits data analysis and interpretation. The absolute orientation of horizontal components are critical for many modern seismic analysis techniques such as receiver functions, body- and surface-wave polarization analysis, studies of anisotropy, and surface wave dispersion curves estimations. The techniques proposed to retrieve the correct sensor horizontal components orientations use different approaches (polarization analysis, cross-correlation measurements, synthetic seismograms fitting), different data set (shots, earthquakes, seismic noise) and different portion of the seismic wave-field (P or S wave arrival times, Rayleigh waves, full waveforms), but are all based on the post-processing of the acquired data. All these methods are not error-free and not always applicable. Method based on active source are not applicable in passive OBS monitoring campaigns. The method based on synthetic waveforms are strong dependent on accuracy of the source parameters estimation and are generally computationally intensive. The method based on polarization analysis are clearly strong dependent on the quality of the data in term of number of seismic events recorded, azimuthal coverage and signal to noise ratio. The methods base on events or noise cross-correlation can be applicable only if an array of sensor

  6. Stratigraphy of a proposed wind farm site southeast of Block Island: Utilization of borehole samples, downhole logging, and seismic profiles

    Sheldon, Dane P. H.

    Seismic stratigraphy, sedimentology, lithostratigraphy, downhole geophysical logging, mineralogy, and palynology were used to study and interpret the upper 70 meters of the inner continental shelf sediments within a proposed wind farm site located approximately two to three nautical miles to the southeast of Block Island, Rhode Island. Core samples and downhole logging collected from borings drilled for geotechnical purposes at proposed wind turbine sites along with seismic surveys in the surrounding area provide the data for this study. Cretaceous coastal plain sediments that consist of non-marine to marine sand, silt, and clay are found overlying bedrock at a contact depth beyond the sampling depth of this study. The upper Cretaceous sediments sampled in borings are correlated with the Magothy/Matawan formations described regionally from New Jersey to Nantucket. An unconformity formed through sub-aerial, fluvial, marine, and glacial erosion marks the upper strata of the Cretaceous sediments separating them from the overlying deposits. The majority of Quaternary deposits overlying the unconformity represent the advance, pulsing, and retreat of the Laurentide ice sheet that reached its southern terminus in the area of Block Island approximately 25,000 to 21,000 years before present. The sequence consists of a basal glacial till overlain by sediments deposited by meltwater environments ranging from deltaic to proglacial lakefloor. A late Pleistocene to early Holocene unconformity marks the top of the glacial sequence and was formed after glacial retreat through fluvial and subaerial erosion/deposition. Overlying the glacial sequence are sediments deposited during the late Pleistocene and Holocene consisting of interbedded gravel, sand, silt, and clay. Sampling of these sediments was limited and surficial reflectors in seismic profiles were masked due to a hard bottom return. However, two depositional periods are interpreted as representing fluvial and estuarine

  7. Voltage- and space-clamp errors associated with the measurement of electrotonically remote synaptic events.

    Spruston, N; Jaffe, D B; Williams, S H; Johnston, D


    1. The voltage- and space-clamp errors associated with the use of a somatic electrode to measure current from dendritic synapses are evaluated using both equivalent-cylinder and morphologically realistic models of neuronal dendritic trees. 2. As a first step toward understanding the properties of synaptic current distortion under voltage-clamp conditions, the attenuation of step and sinusoidal voltage changes are evaluated in equivalent cylinder models. Demonstration of the frequency-dependent attenuation of voltage in the cable is then used as a framework for understanding the distortion of synaptic currents generated at sites remote from the somatic recording electrode and measured in the voltage-clamp recording configuration. 3. Increases in specific membrane resistivity (Rm) are shown to reduce steady-state voltage attenuation, while producing only minimal reduction in attenuation of transient voltage changes. Experimental manipulations that increase Rm therefore improve the accuracy of estimates of reversal potential for electrotonically remote synapses, but do not significantly reduce the attenuation of peak current. In addition, increases in Rm have the effect of slowing the kinetics of poorly clamped synaptic currents. 4. The effects of the magnitude of the synaptic conductance and its kinetics on the measured synaptic currents are also examined and discussed. The error in estimating parameters from measured synaptic currents is greatest for synapses with fast kinetics and large conductances. 5. A morphologically realistic model of a CA3 pyramidal neuron is used to demonstrate the generality of the conclusions derived from equivalent cylinder models. The realistic model is also used to fit synaptic currents generated by stimulation of mossy fiber (MF) and commissural/associational (C/A) inputs to CA3 neurons and to estimate the amount of distortion of these measured currents. 6. Anatomic data from the CA3 pyramidal neuron model are used to construct a

  8. Experimental determination of optimal clamping torque for AB-PEM Fuel cell

    Noor Ul Hassan


    Full Text Available Polymer electrolyte Membrane (PEM fuel cell is an electrochemical device producing electricity by the reaction of hydrogen and oxygen without combustion. PEM fuel cell stack is provided with an appropriate clamping torque to prevent leakage of reactant gases and to minimize the contact resistance between gas diffusion media (GDL and bipolar plates. GDL porous structure and gas permeability is directly affected by the compaction pressure which, consequently, drastically change the fuel cell performance. Various efforts were made to determine the optimal compaction pressure and pressure distributions through simulations and experimentation. Lower compaction pressure results in increase of contact resistance and also chances of leakage. On the other hand, higher compaction pressure decreases the contact resistance but also narrows down the diffusion path for mass transfer from gas channels to the catalyst layers, consequently, lowering cell performance. The optimal cell performance is related to the gasket thickness and compression pressure on GDL. Every stack has a unique assembly pressure due to differences in fuel cell components material and stack design. Therefore, there is still need to determine the optimal torque value for getting the optimal cell performance. This study has been carried out in continuation of deve­lopment of Air breathing PEM fuel cell for small Unmanned Aerial Vehicle (UAV application. Compaction pressure at minimum contact resistance was determined and clamping torque value was calcu­la­ted accordingly. Single cell performance tests were performed at five different clamping torque values i.e 0.5, 1.0, 1.5, 2.0 and 2.5 N m, for achieving optimal cell per­formance. Clamping pressure distribution tests were also performed at these torque values to verify uniform pressure distribution at optimal torque value. Experimental and theoretical results were compared for making inferences about optimal cell perfor­man­ce. A

  9. From galaxy-scale fueling to nuclear-scale feedback: the merger-state of radio galaxies 3C293, 3C305 & 4C12.50

    Emonts, Bjorn; Villar-Martin, Montserrat; Hodgson, Jeff; Brogt, Erik; Tadhunter, Clive; Mahony, Elizabeth; Oosterloo, Tom


    Powerful radio galaxies are often associated with gas-rich galaxy mergers. These mergers may provide the fuel to trigger starburst and active galactic nuclear (AGN) activity. In this Research Note, we study the host galaxies of three seemingly young or re-started radio sources that drive fast outflows of cool neutral hydrogen (HI) gas, namely 3C 293, 3C 305 and 4C 12.50 (PKS 1345+12). Our aim is to link the feedback processes in the central kpc-scale region with new information on the distribution of stars and gas at scales of the galaxy. For this, we use deep optical V-band imaging of the host galaxies, complemented with HI emission-line observations to study their gaseous environments. We find prominent optical tidal features in all three radio galaxies, which confirm previous claims that 3C 293, 3C 305 and 4C 12.50 have been involved in a recent galaxy merger or interaction. Our data show the complex morphology of the host galaxies, and identify the companion galaxies that are likely involved in the merger...

  10. The interaction between radio lobes and hot gas in the nearby radio galaxies 3C285 and 3C442A

    Hardcastle, M J; Worrall, D M; Croston, J H; Evans, D A; Birkinshaw, M; Murray, S S


    We present Chandra observations of two nearby radio galaxies in group environments, 3C285 and 3C442A. The host galaxies of both sources are involved in mergers with nearby massive galaxies, and the hot gas in the systems is extended along lines joining the interacting galaxies. Both sources show strong evidence for interactions between the radio lobes and the asymmetrical hot gas. We argue that the structure in the hot gas is independent of the existence of the radio lobes in these systems, and argue that hot gas shaped by an ongoing massive galaxy merger may play an important role in the dynamics of radio lobes in other objects. For 3C442A, our observations show that gas is being driven out of both members of the host interacting galaxy pair, and the implied constraints on galaxy velocities are consistent with mildly supersonic motions with respect to the group-scale hot gas. The previously known filamentary radio structure in the center of 3C442A may be a result of the interaction between hot gas expelled f...

  11. Design of High Precision Downhole Storage Electronic Pressure Gauge%高精度井下存储式电子压力计的设计

    寇雪芹; 仝美娟; 刘华强; 李希孝; 王伟宣


    In order to more accurately measure and obtain stratum pressure data, the high precision downhole storage electronic pressure gauge was researched.The electronic pressure gauge was composed of the downhole equipment and ground handling The former completed the borehole pressure and temperature collection and storage with ADuC834 MUC as the core.The latter achieved downhole data playback and processing The structure and the indicators of electronic pressure gauge was described.The hardware design and software design of this system were detailed, and the performance of pressure gauge was tested.Testing and field work showed that the instrument could achieve pressure accuracy within 0.05% in the range of -40℃~150℃ to meet the requirements of underground testing%为了更准确地测取地层压力数据,研制了高精度井下存储式电子压力计.该压力计由井下仪器和地面处理2个部分组成:井下仪器以ADuC834单片机为核心,完成井内压力和温度的采集和存储;地面处理实现井下数据回放和处理.详述了该仪器结构和指标、系统硬件设计以及软件设计方案,并对压力计的性能进行测试.测试及现场工作表明,该仪表能够实现-40℃~150℃范围内的压力测量,测压精度达到0.05%,满足井下测试要求.

  12. Conformational analysis of processivity clamps in solution demonstrates that tertiary structure does not correlate with protein dynamics.

    Fang, Jing; Nevin, Philip; Kairys, Visvaldas; Venclovas, Ceslovas; Engen, John R; Beuning, Penny J


    The relationship between protein sequence, structure, and dynamics has been elusive. Here, we report a comprehensive analysis using an in-solution experimental approach to study how the conservation of tertiary structure correlates with protein dynamics. Hydrogen exchange measurements of eight processivity clamp proteins from different species revealed that, despite highly similar three-dimensional structures, clamp proteins display a wide range of dynamic behavior. Differences were apparent both for structurally similar domains within proteins and for corresponding domains of different proteins. Several of the clamps contained regions that underwent local unfolding with different half-lives. We also observed a conserved pattern of alternating dynamics of the α helices lining the inner pore of the clamps as well as a correlation between dynamics and the number of salt bridges in these α helices. Our observations reveal that tertiary structure and dynamics are not directly correlated and that primary structure plays an important role in dynamics.

  13. Doping and stability of 3C-SiC: from thinfilm to bulk growth

    Jokubavicius, V.; Sun, J.; Linnarsson, M. K.

    Cubic silicon carbide (3C-SiC) could pave the way for development of advanced electronic and optoelectronic devices. It could be an excellent substrate for growth of nitride and epitaxial graphene layers. Boron doped 3C-SiC films could reach up to 60% efficiency and pave the way for a new solar...... cell technology. Nitrogen and boron doped 3C-SiC layers can depict a new infrared LED. Hexagonal SiC is an excellent substrate for heteropeitaxial growth of 3C-SiC due to excellent compatibility in lattice constant and thermal expansion coefficient. However, the growth of 3C-SiC on such substrates...... is still being followed by a number of obstacles like polytype stabilization and high density of double positioning boundaries in the grown material. The polytype stability during epitaxial growth of doped 3C-SiC has not been explored. Consequently, the polytype stability during bulk growth of doped 3C...

  14. Tools for electrophysiology labs: a C coded dynamic-clamp on DSP board (aC/DC).

    Boussa, Sofiane; Pasquier, Jennifer; Leboulenger, Francois; Le Foll, Frank; Faure, Alain


    ISBN : 978-2-9532965-0-1; Dynamic-clamp represents an archetypal interdisciplinary approach in the field of computational neuroscience because it aims at establishing a dialog in real time between i) a numerical model and a spiking cell and ii) between neurobiologists and computer programmers. In this paper, we present a relative simple hardware solution for an efficient dynamic-clamp application. The prototype has been built around a DSP board (dsPIC 33F, Microchip INC). Our results demonstr...

  15. Antiferromagnetic resonance in the Mott insulator fcc-Cs3C60.

    Suzuki, Yuta; Shibasaki, Seiji; Kubozono, Yoshihiro; Kambe, Takashi


    The magnetic ground state of the fcc phase of the Mott insulator Cs3C60 was studied using a low-temperature electron spin resonance technique, and antiferromagnetic resonance (AFMR) below 1.57 K was directly observed at ambient pressure. The AFMR modes for the fcc phase of Cs3C60 were investigated using a conventional two-sublattice model with uniaxial anisotropy, and the spin-flop field was determined to be 4.7 kOe at 1.57 K. The static magnetic exchange interactions and anisotropy field for fcc-Cs3C60 were also estimated.

  16. A conserved physical and functional interaction between the cell cycle checkpoint clamp loader and DNA ligase I of eukaryotes.

    Song, Wei; Levin, David S; Varkey, Johnson; Post, Sean; Bermudez, Vladimir P; Hurwitz, Jerard; Tomkinson, Alan E


    DNA ligase I joins Okazaki fragments during DNA replication and completes certain excision repair pathways. The participation of DNA ligase I in these transactions is directed by physical and functional interactions with proliferating cell nuclear antigen, a DNA sliding clamp, and, replication factor C (RFC), the clamp loader. Here we show that DNA ligase I also interacts with the hRad17 subunit of the hRad17-RFC cell cycle checkpoint clamp loader, and with each of the subunits of its DNA sliding clamp, the heterotrimeric hRad9-hRad1-hHus1 complex. In contrast to the inhibitory effect of RFC, hRad17-RFC stimulates joining by DNA ligase I. Similar results were obtained with the homologous Saccharomyces cerevisiae proteins indicating that the interaction between the replicative DNA ligase and checkpoint clamp is conserved in eukaryotes. Notably, we show that hRad17 preferentially interacts with and specifically stimulates dephosphorylated DNA ligase I. Moreover, there is an increased association between DNA ligase I and hRad17 in S phase following DNA damage and replication blockage that occurs concomitantly with DNA damage-induced dephosphorylation of chromatin-associated DNA ligase I. Thus, our results suggest that the in vivo interaction between DNA ligase I and the checkpoint clamp loader is regulated by post-translational modification of DNA ligase I.

  17. Structure-activity relationships of heteroaromatic esters as human rhinovirus 3C protease inhibitors.

    Im, Isak; Lee, Eui Seung; Choi, Soo Jeong; Lee, Ju-Yeon; Kim, Yong-Chul


    Human rhinovirus 3C protease (HRV 3C(pro)) is known to be a promising target for development of therapeutic agents against the common cold because of the importance of the protease in viral replication as well as its expression in a large number of serotypes. To explore non-peptidic inhibitors of HRV 3C(pro), a series of novel heteroaromatic esters was synthesized and evaluated for inhibitory activity against HRV 3C(pro), to determine the structure-activity relationships. The most potent inhibitor, 7, with a 5-bromopyridinyl group, had an IC(50) value of 80nM. In addition, the binding mode of a novel analog, 19, with the 4-hydroxyquinolinone moiety, was explored by molecular docking, suggesting a new interaction in the S1 pocket.

  18. Thermodynamic properties of solid face centered cubic Rb3C60 at high temperature and pressure

    Yang, W.; Sun, J. X.; Liu, H.; Yan, G. F.


    Analytic equation of state and thermodynamic quantities of solid fcc Rb3C60 are derived by using an analytic mean field potential method. For intermolecular forces, the double-exponential potential is utilized. Four potential parameters are determined by fitting experimental compression data of Rb3C60 up to 14 GPa at 296 K. Various physical quantities including isothermals, thermal expansion, isochoric heat capacity, Helmholtz free energy and internal energy are calculated and analyzed. Calculated results are consistent with available experimental data in literature. Furthermore, spinodal temperature for Rb3C60 is found to be 2,860 K. Results verify that analytic mean field potential method is a useful approach to consider the anharmonic effect at high temperatures. Numerous reasonable predictions and the change trend of the properties for Rb3C60 at high temperature and pressure have been given.

  19. Generation of a C3c specific monoclonal antibody and assessment of C3c as a putative inflammatory marker derived from complement factor C3

    Palarasah, Yaseelan; Skjodt, Karsten; Brandt, Jette;


    complex (C5b-C9) and quantification of complement split products by precipitation-in-gel techniques (e.g. C3d). We have developed a mouse monoclonal antibody (mAb) that is able to detect fluid phase C3c without interference from other products generated from the complement component C3. The C3c specific m......Ab was tested in different ELISA combinations with various types of in vitro activated sera and with plasma or serum samples from factor I deficient patients. The specificity of the mAb was evaluated in immunoprecipitation techniques and by analysis of eluted fragments of C3 after immunoaffinity chromatography...

  20. 基于S3C6410的Uboot分析与移植%Uboot Analysis and Transplantation Based on S3C6410

    冯林琳; 耿恒山


    This paper analyzes the structure and the starting process of the new edition Uboot, gives a detailed transplant program based on S3C6410 development board, to achieve Uboot start from NAND Flash. Through the transplant of the net card DM9000A, Uboot network communication function is implemented. The paper has certain reference value for similar platform Uboot transplant.%分析Uboot新版源代码结构和其启动流程,给出基于S3C6410芯片开发板上Uboot的详细移植方案,实现从NANDFlash中启动Uboot.通过对DM9000A网卡的移植,完成Uboot的网络通信功能.对类似平台的Uboot移植有一定的参考价值.

  1. Uboot Analysis and Transplantation Based on S3C2440%基于S3C2440的Uboot分析与移植



    According to the structure and function of the Uboot, and the analysis of starting codes. This paper firstly proposed a transplant program based on s3c2440 high-capacity Nand Flash and Nor Flash. Then, through a multi-step migration, it improved the various functional modules. Eventually, it loaded the kernel and NFS file systems successfully, by the Uboot, given more Details of the Uboot transplantation.%首先根据对Uboot的结构功能和启动分析,提出了一种基于s3c2440大容量Nand Flash和Nor Flash的移植方案,然后通过多步的移植,完善各个功能模块.最终在Uboot下,使用NFS方式成功加载内核和文件系统,详细给出了Uboot移植方法.

  2. Transition from fetal to neonatal circulation: Modeling the effect of umbilical cord clamping.

    Yigit, Mehmet B; Kowalski, William J; Hutchon, David J R; Pekkan, Kerem


    Hemodynamics of the fetal to neonatal transition are orchestrated through complex physiological changes and results in cardiovascular adaptation to the adult biventricular circulation. Clinical practice during this critical period can influence vital organ physiology for normal newborns, premature babies and congenital heart defect patients. Particularly, the timing of the cord clamping procedure, immediate (ICC) vs. delayed cord clamping (DCC), is hypothesized to be an important factor for the transitory fetal hemodynamics. The clinical need for a quantitative understanding of this physiology motivated the development of a lumped parameter model (LPM) of the fetal cardio-respiratory system covering the late-gestation to neonatal period. The LPM was validated with in vivo clinical data and then used to predict the effects of cord clamping procedures on hemodynamics and vital gases. Clinical time-dependent resistance functions to simulate the vascular changes were introduced. For DCC, placental transfusion (31.3 ml) increased neonatal blood volume by 11.7%. This increased blood volume is reflected in an increase in preload pressures by ~20% compared to ICC, which in turn increased the cardiac output (CO) by 20% (COICC=993 ml/min; CODCC=1197 ml/min). Our model accurately predicted dynamic flow patterns in vivo. DCC was shown to maintain oxygenation if the onset of pulmonary respiration was delayed or impaired. On the other hand, a significant 25% decrease in oxygen saturations was observed when applying ICC under the same physiological conditions. We conclude that DCC has a significant impact on newborn hemodynamics, mainly because of the improved blood volume and the sustained placental respiration.

  3. The Extreme Ultraviolet Spectrum of the Kinetically Dominated Quasar 3C 270.1

    Punsly, Brian


    Only a handful of quasars have been identified as kinetically dominated, their long term time averaged jet power, $\\overline{Q}$, exceeds the bolometric thermal emission, $L_{bol}$, associated with the accretion flow. This letter presents the first extreme ultraviolet (EUV) spectrum of a kinetically dominated quasar, 3C 270.1. The EUV continuum flux density of 3C 270.1 is very steep, $F_{\

  4. Target cell APOBEC3C can induce limited G-to-A mutation in HIV-1.

    Khaoula Bourara


    Full Text Available The evolutionary success of primate lentiviruses reflects their high capacity to mutate and adapt to new host species, immune responses within individual hosts, and, in recent years, antiviral drugs. APOBEC3G (A3G and APOBEC3F (A3F are host cell DNA-editing enzymes that induce extensive HIV-1 mutation that severely attenuates viral replication. The HIV-1 virion infectivity factor (Vif, expressed in vivo, counteracts the antiviral activity of A3G and A3F by inducing their degradation. Other APOBECs may contribute more to viral diversity by inducing less extensive mutations allowing viral replication to persist. Here we show that in APOBEC3C (A3C-expressing cells infected with the patient-derived HIV-1 molecular clones 210WW, 210WM, 210MW, and 210MM, and the lab-adapted molecular clone LAI, viral G-to-A mutations were detected in the presence of Vif expression. Mutations occurred primarily in the GA context and were relatively infrequent, thereby allowing for spreading infection. The mutations were absent in cells lacking A3C but were induced after transient expression of A3C in the infected target cell. Inhibiting endogenous A3C by RNA interference in Magi cells prevented the viral mutations. Thus, A3C is necessary and sufficient for G-to-A mutations in some HIV-1 strains. A3C-induced mutations occur at levels that allow replication to persist and may therefore contribute to viral diversity. Developing drugs that inhibit A3C may be a novel strategy for delaying viral escape from immune or antiretroviral inhibition.

  5. Target Cell APOBEC3C Can Induce Limited G-to-A Mutation in HIV-1

    Bourara, Khaoula; Liegler, Teri J; Grant, Robert M


    The evolutionary success of primate lentiviruses reflects their high capacity to mutate and adapt to new host species, immune responses within individual hosts, and, in recent years, antiviral drugs. APOBEC3G (A3G) and APOBEC3F (A3F) are host cell DNA-editing enzymes that induce extensive HIV-1 mutation that severely attenuates viral replication. The HIV-1 virion infectivity factor (Vif), expressed in vivo, counteracts the antiviral activity of A3G and A3F by inducing their degradation. Other APOBECs may contribute more to viral diversity by inducing less extensive mutations allowing viral replication to persist. Here we show that in APOBEC3C (A3C)-expressing cells infected with the patient-derived HIV-1 molecular clones 210WW, 210WM, 210MW, and 210MM, and the lab-adapted molecular clone LAI, viral G-to-A mutations were detected in the presence of Vif expression. Mutations occurred primarily in the GA context and were relatively infrequent, thereby allowing for spreading infection. The mutations were absent in cells lacking A3C but were induced after transient expression of A3C in the infected target cell. Inhibiting endogenous A3C by RNA interference in Magi cells prevented the viral mutations. Thus, A3C is necessary and sufficient for G-to-A mutations in some HIV-1 strains. A3C-induced mutations occur at levels that allow replication to persist and may therefore contribute to viral diversity. Developing drugs that inhibit A3C may be a novel strategy for delaying viral escape from immune or antiretroviral inhibition. PMID:17967058

  6. Microstructure and mechanical properties of thermal sprayed nanostructured Cr3C2-Ni20Cr coatings

    Cecilio Alvares da Cunha; Nelson Batista de Lima; Jose Roberto Martinelli; Ana Helena de Almeida Bressiani; Armando Guilherme Fernando Padial; Lalgudi Venkataraman Ramanathan


    Cr3C2-Ni20Cr coatings have been used for corrosion and wear resistant applications. However, one of the shortcomings of these coatings is its low hardness, and consequent low wear resistance, for long term high temperature applications. Nanostructured coatings of many materials have exhibited higher hardness and strength compared with conventional coatings of the same material. Consequently, nanostructured coatings of other materials, including Cr3C2-Ni20Cr have been attempted to enhance over...

  7. Association between variants of 5-hydroxytryptamine receptor 3C (HTR3C) and chemotherapy-induced symptoms in women receiving adjuvant treatment for breast cancer.

    Pud, Dorit; Har-Zahav, Gil; Laitman, Yael; Rubinek, Tami; Yeheskel, Adva; Ben-Ami, Sarah; Kaufman, Bella; Friedman, Eitan; Symon, Zvi; Wolf, Ido


    Administration of chemotherapy is associated with a wide array of symptoms affecting quality of life. Genetic risk factors for severity of chemotherapy-induced symptoms have not been determined. The present study aimed to explore the associations between polymorphisms in candidate genes and chemotherapy-induced symptoms. Women treated with at least two cycles of adjuvant doxorubicin and cyclophosphamide, with or without paclitaxel for early breast cancer (n = 105) completed the memorial symptom assessment scale and provided blood for genotyping. DNA was extracted from peripheral blood leukocytes and assayed for single nucleotide polymorphisms (SNPs) in GTP cyclohydrolase 1 (GCH1, rs10483639, rs3783641, and rs8007267), catecholamine-o-methyltransferase (COMT, rs4818), and 5-hydroxytryptamine (serotonin) receptor 3C (HTR3C, rs6766410, and rs6807362). Genotyping of HTR3C revealed a significant association between the presence of rs6766410 and rs6807362 SNPs (K163 and G405 variants) and increased severity of symptoms (p = 0.0001 and p = 0.007, respectively). Multiple regressions revealed that rs6766410 and rs6807362, but not age or stage at diagnosis, predicted severity of symptoms (p = 0.001 and p = 0.006, respectively) and explained 12 % of the variance in each regression model. No association was found between the genetic variants of CGH1 or COMT and symptom score. Our study indicates, for the first time, an association between variants of HTR3C and severity of chemotherapy-induced symptoms. Analyzing these genetic variants may identify patients at increased risk for the development of chemotherapy-induced symptoms and targeting the serotonin pathway may serve as a novel treatment strategy for these patients.

  8. The Analysis of Clamping Mechanism Theory and the Application Design of Tobacco Leaves'Clamping Tool%夹紧机构的理论分析及烘烤烟叶夹的应用设计

    徐远龙; 孙有祥


    In view of the complicated manual skill of the tobacco leaf weaving, the study and design of a kind tool clamping tobacco leaf is proposed by our actual investigation; According to the predetermined function demand and the available clamping mechanism, the principle of the clamping tool is studied out, the structural design scheme of the clamping tool is worked out and the clamping tool is made by the theory analysis of mechanism design and the study of clamping mechanism. The clamping tool tested by the surveyor is good in use, which is welcome to use by the peasants and the economy benefit of the market can be achieved highly.%通过对烟叶烘烤生产工艺的实际调研,针对手工繁杂的编烟技术,提出了研究设计一种烘烤烟叶夹持工具;针对烘烤烟叶夹的预定功能要求,参考现有的实用夹紧机构,通过对机械产品机构设计的理论分析和夹紧机构的力学研究,确定了夹紧烟叶的力学原理,提出了烘烤烟叶夹的结构设计方案,设计制造出烘烤烟叶夹;该烘烤烟叶夹通过了产品的质量检测,使用效果良好,烟农用户普遍接受使用,预计有较高的市场经济效益.

  9. Enhanced Buck-Boost Three-Level Neutral-Point-Clamped Inverters

    Tan, K. K.; Gao, F.; Chiang Loh, Poh


    In traditional three-level neutral-point-clamped (NPC) inverters, a major issue is capacitor voltage imbalance, which results in low order harmonics. The compensation of the capacitor voltages often require additional control complexity, which cannot be conveniently implemented. The "alternative...... method, with the ability to easily adjust for capacitor voltage imbalances. To further improve the boost capability of the three-level NPC inverters, another new topology introduces 2 additional diodes,achieving higher boost performance while totally eliminating thepossibility of capacitor voltage...

  10. Characterization of the insulin sensitivity of ghrelin receptor KO mice using glycemic clamps

    Morgan Kristen


    Full Text Available Abstract Background We and others have demonstrated previously that ghrelin receptor (GhrR knock out (KO mice fed a high fat diet (HFD have increased insulin sensitivity and metabolic flexibility relative to WT littermates. A striking feature of the HFD-fed GhrR KO mouse is the dramatic decrease in hepatic steatosis. To characterize further the underlying mechanisms of glucose homeostasis in GhrR KO mice, we conducted both hyperglycemic (HG and hyperinsulinemic-euglycemic (HI-E clamps. Additionally, we investigated tissue glucose uptake and specifically examined liver insulin sensitivity. Results Consistent with glucose tolerance-test data, in HG clamp experiments, GhrR KO mice showed a reduction in glucose-stimulated insulin release relative to WT littermates. Nevertheless, a robust 1st phase insulin secretion was still achieved, indicating that a healthy β-cell response is maintained. Additionally, GhrR KO mice demonstrated both a significantly increased glucose infusion rate and significantly reduced insulin requirement for maintenance of the HG clamp, consistent with their relative insulin sensitivity. In HI-E clamps, both LFD-fed and HFD-fed GhrR KO mice showed higher peripheral insulin sensitivity relative to WT littermates as indicated by a significant increase in insulin-stimulated glucose disposal (Rd, and decreased hepatic glucose production (HGP. HFD-fed GhrR KO mice showed a marked increase in peripheral tissue glucose uptake in a variety of tissues, including skeletal muscle, brown adipose tissue and white adipose tissue. GhrR KO mice fed a HFD also showed a modest, but significant decrease in conversion of pyruvate to glucose, as would be anticipated if these mice displayed increased liver insulin sensitivity. Additionally, the levels of UCP2 and UCP1 were reduced in the liver and BAT, respectively, in GhrR KO mice relative to WT mice. Conclusions These results indicate that improved glucose homeostasis of GhrR KO mice is

  11. Modeling of Busbars in High Power Neutral Point Clamped Three-Level Inverters

    YI Rung; ZHAO Zhengming; ZHONG Yulin


    The busbars in high power neutral point clamped three-level inverters are modeled using the Maxwell Q3D Extractor software, which is based on the partial element equivalent circuits method. The equivalent circuits of the busbars and devices model are simulated in the electric simulator PSIM to analyze the effects of the parasitic inductance on the switching characteristics of the integrated gate commutatedthyristor (IGCT) in different topology positions. The simulation results agree well with the measured imped-ance analyzer results and the IGCT test results, which proves the effectiveness of the modeling method for the large, complex busbars.

  12. Dynamic Gain-Clamped Amplifier with Backward-Injection of a Fabry-Perot Laser

    Chien-Hung Yeh; Chih-Yang Chen; Chien-Chung Lee; Sien Chi


    We have been experimentally demonstrated an active control technique of dynamic gain-clamped spectra for the erbium-doped fiber amplifiers (EDFA's) by a backward-injected Fabry-Perot (F-P) laser into this EDFA. In addition, employing a short length erbium-doped fiber (EDF) that not cause any gain saturation for preamplification in front of this amplifier module, it can reduce the noise figure degradation and simultaneously achieve gain variation from 11.1 dB to 0.5 dB for 10 dB input power level change.

  13. Dynamic Gain-Clamped Amplifier with Backward-Injection of a Fabry-Perot Laser

    Chien-Hung; Yeh; Chih-Yang; Chen; Chien-Chung; Lee; Sien; Chi


    We have been experimentally demonstrated an active control technique of dynamic gain-clamped spectra for theerbium-doped fiber amplifiers (EDFA's) by a backward-injected Fabry-Perot (F-P) laser into this EDFA. In addition, employing a short length erbium-doped fiber (EDF) that not cause any gain saturation for preamplification in front of this amplifier module, it can reduce the noise figure degradation and simultaneously achieve gain variation from 11.1 dB to 0.5 dB for 10 dB input power level change.

  14. Longitudinal relaxation of mechanically clamped KH2PO4 type crystals

    R.R. Levitskii


    Full Text Available Within the framework of a modified proton ordering model of the KH2PO4 family ferroelectric crystals, taking into account a linear over the strain ϵ6 contribution into the proton system energy, we obtain an expression for longitudinal dynamic dielectric permittivity of a mechanically clamped crystal using the four-particle cluster approximation and the dynamic Glauber approach. At a proper choice of the model parameters, we obtain a good quantitative description of available experimental data for these crystals.

  15. Effect of La3+ on myocardiac potassium channels revealed by patch-clamp technique

    XUE Shaowu; YANG Pin


    The effect of La3+ on potassium channels in rat ventricular myocytes was investigated using the whole-cell patch-clamp recording mode. The Ca2+-independent voltage- activated outward K+ current was activated by the depolarizing pulse in enzymatically isolated rat ventricular myocytes. After addition of different concentrations La3+ to the bath solution, the outward K+ current was depressed gradually. The inhibition effect was in a concentration-dependent manner. The phenomena of the outward K+ current, being the main repolarizing current suppressed by La3+, suggest that the effect of lanthanides on myocardial function should be exploited further.

  16. Five-Level Z-Source Neutral Point-Clamped Inverter

    Gao, F.; Loh, P.C.; Blaabjerg, Frede;


    -through scheme reveals the hidden theories in the five-level Z-source NPC inverter unlike the operational principle appeared in the general two-level Z-source inverter, so that the five-level Z-source NPC inverter can be designed with the modulation of carrier-based phase disposition (PD) or alternative phase......This paper proposes a five-level Z-source neutralpoint- clamped (NPC) inverter with two Z-source networks functioning as intermediate energy storages coupled between dc sources and NPC inverter circuitry. Analyzing the operational principles of Z-source network with partial dclink shoot...

  17. Direct-conversion switching-mode audio power amplifier with active capacitive voltage clamp

    Ljusev, P.; Andersen, Michael A.E.


    This paper discusses the advantages and problems when implementing direct energy conversion switching-mode audio power amplifiers. It is shown that the total integration of the power supply and Class D audio power amplifier into one compact direct converter can simplify design, increase efficiency and integration level, reduce product volume and lower its cost. As an example, the principle of operation and the measurements made on a direct-conversion switching-mode audio power amplifier with active capacitive voltage clamp are presented. (au)

  18. Transverse Vibrations of Clamped and Simply-Supported Circular Plates with Two Dimensional Thickness Variation

    N. Bhardwaj


    Full Text Available Two dimensional boundary characteristic orthonormal polynomials are used in the Ritz method for the vibration analysis of clamped and simply-supported circular plates of varying thickness. The thickness variation in the radial direction is linear whereas in the circumferential direction the thickness varies according to cos kθ, where k is an integer. In order to verify the validity, convergence and accuracy of the results, comparison studies are made against existing results for the special case of linearly tapered thickness plates. Variations in frequencies for the first six normal modes of vibration and mode shapes for various taper parameters are presented.

  19. Surface analysis of anodized aluminum clamps from NASA-LDEF satellite

    Grammer, H. L.; Wightman, J. P.; Young, Philip R.


    Surface analysis results of selected anodized aluminum clamps containing black (Z306) and white (A276) paints which received nearly six years of Low Earth Orbit (LEO) exposure on the Long Duration Exposure Facility are reported. Surface analytical techniques, including x-ray photoelectron spectroscopy (XPS), Auger electron spectroscopy (AES), and scanning electron microscopy/energy dispersive analysis by x-ray (SEM/EDAX), showed significant differences in the surface composition of these materials depending upon the position on the LDEF. Differences in the surface composition are attributed to varying amounts of atomic oxygen and vacuum ultraviolet radiation (VUV). Silicon containing compounds were the primary contaminant detected on the materials.

  20. Characterization of the IEC 61000-4-6 Electromagnetic Clamp for Conducted-Immunity Testing

    Grassi, F.; Pignari, S. A.; Spadacini, G.; Toscani, N.; Pelissou, P.


    A multiconductor transmission line model (MTL) is used to investigate the operation of the IEC 61000-4-6 electromagnetic (EM) clamp in a conducted-immunity test setup for aerospace applications. Aspects of interest include the performance of such a coupling device at very high frequencies (up to 1 GHz), and for extreme values of the common-mode impedance of equipment (short circuits, open circuits). The MTL model is finally exploited to predict the frequency response of coupling and decoupling factors defined in the IEC 61000-4-6 standard.

  1. The Elg1 Clamp Loader Plays a Role in Sister Chromatid Cohesion

    Oren Parnas; Adi Zipin-Roitman; Yuval Mazor; Batia Liefshitz; Shay Ben-Aroya; Martin Kupiec


    Mutations in the ELG1 gene of yeast lead to genomic instability, manifested in high levels of genetic recombination, chromosome loss, and gross chromosomal rearrangements. Elg1 shows similarity to the large subunit of the Replication Factor C clamp loader, and forms a RFC-like (RLC) complex in conjunction with the 4 small RFC subunits. Two additional RLCs exist in yeast: in one of them the large subunit is Ctf18, and in the other, Rad24. Ctf18 has been characterized as the RLC that functions ...

  2. Single stage buck-boost DC-AC neutral point clamped inverter

    Mo, Wei; Loh, Poh Chiang; Andrew, A.;


    This paper proposes a new single stage buck-boost DC-AC neutral point clamped inverter topology which integrates the cascaded configurations of recently introduced inductor-capacitor-capacitor-transformer impedance source network (by Adamowicz) and classic NPC configuration. As a consequence...... windings thus preventing the core from saturation; lowers the voltage stresses and power losses of inverter switches and reduces the sizes of filtering devices and as well as obtains better output performance compared to the original two-level Z-source inverters. A phase disposition pulse width modulation...

  3. Cyclic peptide inhibitors of the β-sliding clamp in Staphylococcus aureus

    Kjelstrup, Susanne; Hansen, Paula Melo Paulon; Thomsen, Line Elnif;


    Interaction between pairs of Staphylococcus aureus replication proteins was detected in an Escherichia coli based two-hybrid analysis. A reverse two-hybrid system was constructed for selection of compounds that hindered interaction between interacting protein pairs. A number of cyclic peptides......, from a library generated by the split intein-mediated circular ligation of peptides and proteins technology, were found to interfere with dimerization of the β-sliding clamp of the replisome. Two 8-mer peptides were analyzed in more detail. Both inhibited DNA replication, led to SOS induction, altered...

  4. Five-Level Active-Neutral-Point-Clamped DC/DC Converter

    Liu, Dong; Deng, Fujin; Chen, Zhe


    reliability. Furthermore, a capacitor voltage control strategy by alternating two operation modes of the proposed modulation strategy is proposed to balance the voltage of the flying capacitor, which ensures multi-level voltages producing. Finally, the performance of the proposed converter and control......Multi-level converters are getting more and more attentions because of their obvious merits such as lower voltage stress and harmonic, smaller size of output filters, and so on. In this paper, a five-level active-neutral-point-clamped (5L-ANPC) dc/dc converter is proposed for power transfer...

  5. Picornaviral 3C cysteine proteinases have a fold similar to the chymotrypsin-like serine proteinases

    Allaire,M.; Chernaia, M.; Malcolm, B.; James, M.


    The picornavirus family includes several pathogens such as poliovirus, rhinovirus (the major cause of the common cold), hepatitis A virus and the foot-and-mouth disease virus. Picornaviral proteins are expressed by direct translation of the genomic RNA into a single, large polyprotein precursor. Proteolysis of the viral polyprotein into the mature proteins is assured by the viral 3C enzymes, which are cysteine proteinases. Here we report the X-ray crystal structure at 2.3 {angstrom} resolution of the 3C proteinase from hepatitis A virus (HAV-3C). The overall architecture of HAV-3C reveals a fold resembling that of the chymotrypsin family of serine proteinases, which is consistent with earlier predictions. Catalytic residues include Cys 172 as nucleophile and His 44 as general base. The 3C cleavage specificity for glutamine residues is defined primarily by His 191. The overall structure suggests that an inter-molecular (trans) cleavage releases 3C and that there is an active proteinase in the polyprotein.

  6. Synthesis and electrochemical performance of Ti3C2Tx with hydrothermal process

    Wang, Libo; Zhang, Heng; Wang, Bo; Shen, Changjie; Zhang, Chuanxiang; Hu, Qianku; Zhou, Aiguo; Liu, Baozhong


    In this study, a simple hydrothermal method has been developed to prepare Ti3C2Tx from Ti3AlC2 as a high-performance electrode material for supercapacitors. This method is environmentally friendly and has a low level of danger. The morphology and structure of the Ti3C2Tx can be controlled by hydrothermal reaction time, temperature and NH4F amounts. The prepared Ti3C2Tx was characterized by X-ray diffraction, field emission scanning electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy and Brunauer-Emmet-Teller. The results show that the prepared Ti3C2Tx is terminated by O, OH, and F groups. The electrochemical properties of the Ti3C2Tx sample exhibit specific capacitance up to 141 Fcm-3 in 3 M KOH aqueous electrolyte, and even after 1000 cycles, no significant degradation of the volumetric capacitance was observed. These results indicate that the Ti3C2Tx material prepared by this hydrothermal method can be used in high performance supercapacitors.

  7. Design of Multimedia System Based on S3C6410%基于S3C6410的多媒体系统设计

    雷丰中; 刘鹏


    提出并实现了以S3C6410为核心处理器的嵌入式多媒体系统.详细介绍了多媒体系统整体设计方案、接口资源和S3C6410用于多媒体数据处理的BIT处理器.同时为方便人机交互控制,采用BC7210芯片以一种新的方式设计了红外遥控电路.实验测试表明,该系统为嵌入武多媒体系统开发提供了速度快、稳定性高的系统环境.%In this paper,the embedded multimedia system based on the processor-S3C6410 is presented and implemented. The overall design of multimedia system' s details and interfaces resources and the BIT processor used for multimedia data are given. At the same time,in order to facilitate the interactive control machine by human,a new way of using BC7210 chip to design the infrared remote control circuit is presented. Experimental tests show that the development system provide a fast,high stability system environment for embedded multimedia system development.

  8. Novel Downhole Electromagnetic Flowmeter for Oil-Water Two-Phase Flow in High-Water-Cut Oil-Producing Wells

    Yanjun Wang


    Full Text Available First, the measuring principle, the weight function, and the magnetic field of the novel downhole inserted electromagnetic flowmeter (EMF are described. Second, the basic design of the EMF is described. Third, the dynamic experiments of two EMFs in oil-water two-phase flow are carried out. The experimental errors are analyzed in detail. The experimental results show that the maximum absolute value of the full-scale errors is better than 5%, the total flowrate is 5–60 m3/d, and the water-cut is higher than 60%. The maximum absolute value of the full-scale errors is better than 7%, the total flowrate is 2–60 m3/d, and the water-cut is higher than 70%. Finally, onsite experiments in high-water-cut oil-producing wells are conducted, and the possible reasons for the errors in the onsite experiments are analyzed. It is found that the EMF can provide an effective technology for measuring downhole oil-water two-phase flow.

  9. CO2FieldLab project. Near-surface downhole electrical resistivity monitoring for CO2 shallow injection at the Svelvik ridge (Norway).

    Denchik, N.; Pezard, P.; Paris, J.; Geeraert, M.; Henry, G.; Baqué, L.; Barry, S.; Neyens, D.


    The CO2FieldLab shallow injection experiment is a collaborative effort aimed at developing, verifying, and evaluating near-surface monitoring techniques for geological sequestration of CO2. The objective is to create a downhole leakage of CO2 in order to demonstrate whether existing monitoring techniques have the ability to detect and quantify the CO2 loss. The CO2FieldLab project associates collaboration from several research groups and organizations (SINTEF, NGI, BRGM, BGS, CNRS, imaGeau and Schlumberger). For this, the field Laboratory for monitoring CO2 migration and leakage was established in Holocene deposits of the Svelvik ridge, located in the Drammensfjord 50 km south of Oslo (Norway). It is a glaciofluvial-glaciomarine terminal deposit formed during the last deglaciation. The depth to the bedrock is located between 300 and 400 m. The central part of the ridge is aerially exposed up to 70 m above sea level, constituting a phreatic aquifer. The characterization phase and associated modelling suggest that the site is suitable for studying both gas migration and leakage at shallow depths. The shallow aquifer (down to 50 m) consists in a relatively homogeneous sand body with a depositional dip of about 10° to the North. The shallow injection experiment took place in September of 2011 and consisted in an injection of CO2 at a depth of 20 m from a 45° inclined well. The purpose of this phase was to simulate a point source leakage, which could possibly occur due to failure of a deep well completion. A total mass of 1.67 ton of CO2 was injected over a period of 6 days. The water table was located at 60 cm depth during the experiment and a transition from fresh to salt pore water was found below 12 m depth. An integrated set of surface and downhole strategies was deployed across a 64 m2 square monitoring area: cross-hole radar, water and gas phases physico-chemical parameters (BRGM); multi-hole electrical ALERT system (BGS), CO2 concentration in soils and gas

  10. Gain Clamped Two-Stage Double-Pass L-Band EDFA with a Single Fibre Bragg Grating

    Sulaiman Wadi Harun; Harith Ahmad


    A gain clamping is demonstrated in a two-stage double-pass L-band erbium-doped fibre amplifier (EDFA) by using a single fibre Bragg grating. Two types of the fibre-Bragg-grating (FBG) broadband and narrowband have been used in the gain-clamped system. The combinatiox of the FBG and the optical circulator has created a laser in the cavity for gain clamping. The application ora broadband FBG has improved the amplifier's efficiency and noise figure compared to the narrowband FBG system. The gain is clamped at about 22.1 dB for the amplifier with broadband FBG with gain variation of less than ±0.15 dB and the input saturation power Psat at -8 dBm.The gain varies from 19.0 to 25.4dB in the signal wavelength region of 1574nm-1604nm. The noise figure varies from 6.0 to 10. 8dB at this wavelength range. The noise figure values are found to improve by as much as 0.3-1.1 dB when compared to the unclamped amplifier for signal wavelengths above 1580nm. The high clamped gain and low noise figure are obtained due to the suppression of the backward C-band amplified spontaneous emission. The gain clamped amplifier shows only a small power excursions when three of the four channels are added/dropped. The effects of relaxation oscillation are not seen for the gain-clamped two-stage double-pass L-band EDFA.

  11. A dnaN plasmid shuffle strain for rapid in vivo analysis of mutant Escherichia coli β clamps provides insight into the role of clamp in umuDC-mediated cold sensitivity.

    Vignesh M P Babu

    Full Text Available The E. coli umuDC gene products participate in two temporally distinct roles: UmuD2C acts in a DNA damage checkpoint control, while UmuD'2C, also known as DNA polymerase V (Pol V, catalyzes replication past DNA lesions via a process termed translesion DNA synthesis. These different roles of the umuDC gene products are managed in part by the dnaN-encoded β sliding clamp protein. Co-overexpression of the β clamp and Pol V severely blocked E. coli growth at 30°C. We previously used a genetic assay that was independent of the ability of β clamp to support E. coli viability to isolate 8 mutant clamp proteins (βQ61K, βS107L, βD150N, βG157S, βV170M, βE202K, βM204K and βP363S that failed to block growth at 30°C when co-overexpressed with Pol V. It was unknown whether these mutant clamps were capable of supporting E. coli viability and normal umuDC functions in vivo. The goals of this study were to answer these questions. To this end, we developed a novel dnaN plasmid shuffle assay. Using this assay, βD150N and βP363S were unable to support E. coli viability. The remaining 6 mutant clamps, each of which supported viability, were indistinguishable from β+ with respect to umuDC functions in vivo. In light of these findings, we analyzed phenotypes of strains overexpressing either β clamp or Pol V alone. The strain overexpressing β+, but not those expressing mutant β clamps, displayed slowed growth irrespective of the incubation temperature. Moreover, growth of the Pol V-expressing strain was modestly slowed at 30°, but not 42°C. Taken together, these results suggest the mutant clamps were identified due to their inability to slow growth rather than an inability to interact with Pol V. They further suggest that cold sensitivity is due, at least in part, to the combination of their individual effects on growth at 30°C.

  12. A dnaN plasmid shuffle strain for rapid in vivo analysis of mutant Escherichia coli β clamps provides insight into the role of clamp in umuDC-mediated cold sensitivity.

    Babu, Vignesh M P; Sutton, Mark D


    The E. coli umuDC gene products participate in two temporally distinct roles: UmuD2C acts in a DNA damage checkpoint control, while UmuD'2C, also known as DNA polymerase V (Pol V), catalyzes replication past DNA lesions via a process termed translesion DNA synthesis. These different roles of the umuDC gene products are managed in part by the dnaN-encoded β sliding clamp protein. Co-overexpression of the β clamp and Pol V severely blocked E. coli growth at 30°C. We previously used a genetic assay that was independent of the ability of β clamp to support E. coli viability to isolate 8 mutant clamp proteins (βQ61K, βS107L, βD150N, βG157S, βV170M, βE202K, βM204K and βP363S) that failed to block growth at 30°C when co-overexpressed with Pol V. It was unknown whether these mutant clamps were capable of supporting E. coli viability and normal umuDC functions in vivo. The goals of this study were to answer these questions. To this end, we developed a novel dnaN plasmid shuffle assay. Using this assay, βD150N and βP363S were unable to support E. coli viability. The remaining 6 mutant clamps, each of which supported viability, were indistinguishable from β+ with respect to umuDC functions in vivo. In light of these findings, we analyzed phenotypes of strains overexpressing either β clamp or Pol V alone. The strain overexpressing β+, but not those expressing mutant β clamps, displayed slowed growth irrespective of the incubation temperature. Moreover, growth of the Pol V-expressing strain was modestly slowed at 30°, but not 42°C. Taken together, these results suggest the mutant clamps were identified due to their inability to slow growth rather than an inability to interact with Pol V. They further suggest that cold sensitivity is due, at least in part, to the combination of their individual effects on growth at 30°C.

  13. Mutations of 3c and spike protein genes correlate with the occurrence of feline infectious peritonitis.

    Bank-Wolf, Barbara Regina; Stallkamp, Iris; Wiese, Svenja; Moritz, Andreas; Tekes, Gergely; Thiel, Heinz-Jürgen


    The genes encoding accessory proteins 3a, 3b, 3c, 7a and 7b, the S2 domain of the spike (S) protein gene and the membrane (M) protein gene of feline infectious peritonitis virus (FIPV) and feline enteric coronavirus (FECV) samples were amplified, cloned and sequenced. For this faeces and/or ascites samples from 19 cats suffering from feline infectious peritonitis (FIP) as well as from 20 FECV-infected healthy cats were used. Sequence comparisons revealed that 3c genes of animals with FIP were heavily affected by nucleotide deletions and point mutations compared to animals infected with FECV; these alterations resulted either in early termination or destruction of the translation initiation codon. Two ascites-derived samples of cats with FIP which displayed no alterations of ORF3c harboured mutations in the S2 domain of the S protein gene which resulted in amino acid exchanges or deletions. Moreover, changes in 3c were often accompanied by mutations in S2. In contrast, in samples obtained from faeces of healthy cats, the ORF3c was never affected by such mutations. Similarly ORF3c from faecal samples of the cats with FIP was mostly intact and showed only in a few cases the same mutations found in the respective ascites samples. The genes encoding 3a, 3b, 7a and 7b displayed no mutations linked to the feline coronavirus (FCoV) biotype. The M protein gene was found to be conserved between FECV and FIPV samples. Our findings suggest that mutations of 3c and spike protein genes correlate with the occurrence of FIP.

  14. A Clamped Be Window for the Dump of the HiRadMat Experiment at CERN

    Delonca, M; Grenier, D; Maglioni, C; Sarrio Martinez, A


    At CERN, the High Radiation to Materials facility (HiRadMat) is designed to test accelerator components under the impact of high-intensity pulsed beams and will start operation in 2012. In this frame an LHC TED-type dump was installed at the end of the line, working in nitrogen over-pressure, and a 254μm-thick beryllium window was placed as barrier between the inside of the dump and the external atmosphere. Because of the special loading conditions, a clamped window design was especially developed, optimized and implemented, the more standard welded window not being suitable for such loads. Considering then the clamping force and the applied differential pressures, the stresses on the window components were carefully evaluated thanks to empirical as well as numerical models, to guarantee the structural integrity of the beryllium foil. This paper reports on choices and optimizations that led to the final design, presenting also comparative results from different solutions and the detailed results for the adop...

  15. A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode

    Jergic, Slobodan


    Processive DNA synthesis by the αÉ"θ core of the Escherichia coli Pol III replicase requires it to be bound to the β 2 clamp via a site in the α polymerase subunit. How the É" proofreading exonuclease subunit influences DNA synthesis by α was not previously understood. In this work, bulk assays of DNA replication were used to uncover a non-proofreading activity of É". Combination of mutagenesis with biophysical studies and single-molecule leading-strand replication assays traced this activity to a novel β-binding site in É" that, in conjunction with the site in α, maintains a closed state of the αÉ"θ-β 2 replicase in the polymerization mode of DNA synthesis. The É"-β interaction, selected during evolution to be weak and thus suited for transient disruption to enable access of alternate polymerases and other clamp binding proteins, therefore makes an important contribution to the network of protein-protein interactions that finely tune stability of the replicase on the DNA template in its various conformational states. © 2013 European Molecular Biology Organization.

  16. Correlation of open cell-attached and excised patch clamp techniques.

    Filipovic, D; Hayslett, J P


    The excised patch clamp configuration provides a unique technique for some types of single channel analyses, but maintenance of stable, long-lasting preparations may be confounded by rundown and/or rapid loss of seal. Studies were performed on the amiloride-sensitive Na+ channel, located on the apical surface of A6 cells, to determine whether the nystatin-induced open cell-attached patch could serve as an alternative configuration. Compared to excised inside-out patches, stable preparations were achieved more readily with the open cell-attached patch (9% vs. 56% of attempts). In both preparations, the current voltage (I-V) relation was linear, current amplitudes were equal at opposite equivalent clamped voltages, and Erev was zero in symmetrical Na+ solutions, indicating similar Na+ activities on the cytosolic and external surfaces of the patch. Moreover, there was no evidence that nystatin altered channel activity in the patch because slope conductance (3-4pS) and Erev (75 mV), when the bath was perfused with a high K:low Na solution (ENa = 80 mV), were nearly equal in both patch configurations. Our results therefore indicate that the nystatin-induced open cell-attached patch can serve as an alternative approach to the excised inside-out patch when experiments require modulation of univalent ions in the cytosol.

  17. Testing of Diode-Clamping in an Inductive Pulsed Plasma Thruster Circuit

    Toftul, Alexandra; Polzin, Kurt A.; Martin, Adam K.; Hudgins, Jerry L.


    Testing of a 5.5 kV silicon (Si) diode and 5.8 kV prototype silicon carbide (SiC) diode in an inductive pulsed plasma thruster (IPPT) circuit was performed to obtain a comparison of the resulting circuit recapture efficiency,eta(sub r), defined as the percentage of the initial charge energy remaining on the capacitor bank after the diode interrupts the current. The diode was placed in a pulsed circuit in series with a silicon controlled rectifier (SCR) switch, and the voltages across different components and current waveforms were collected over a range of capacitor charge voltages. Reverse recovery parameters, including turn-off time and peak reverse recovery current, were measured and capacitor voltage waveforms were used to determine the recapture efficiency for each case. The Si fast recovery diode in the circuit was shown to yield a recapture efficiency of up to 20% for the conditions tested, while the SiC diode further increased recapture efficiency to nearly 30%. The data presented show that fast recovery diodes operate on a timescale that permits them to clamp the discharge quickly after the first half cycle, supporting the idea that diode-clamping in IPPT circuit reduces energy dissipation that occurs after the first half cycle

  18. Robotic partial nephrectomy with cold ischemia and on-clamp tumor extraction: recapitulating the open approach.

    Rogers, Craig G; Ghani, Khurshid R; Kumar, Ramesh K; Jeong, Wooju; Menon, Mani


    We describe a reproducible technique for achieving cold ischemia with intraoperative tumor assessment during robotic partial nephrectomy (RPN) that recapitulates the open approach: intracorporeal cooling and extraction (ICE). A total of seven patients underwent the ICE modification of RPN by transperitoneal (n=5) and retroperitoneal (n=2) approaches. A Gelpoint access port was used for the camera and assistant ports. Following hilar clamping, ice slush was introduced through the Gelpoint via syringes and applied over the kidney surface. The excised tumor was immediately extracted through the Gelpoint, allowing gross margin assessment by pathology during the renorrhaphy. RPN was achieved in all cases with successful introduction of ice slush and tumor extraction while on clamp. Median RENAL nephrometry score was 8 (range: 6-10), and there was one solitary kidney. Mean cold ischemia time was 19.6 min (range: 8-37) and mean estimated blood loss was 296.4 ml (range: 50-1000). Renal parenchymal temperatures 0.5°C during any procedures. Intraoperative assessment of the excised tumor showed adequate gross margins in all cases and final pathology confirmed negative surgical margins.

  19. A Clamped Be Window for the Dump of the HiRadMat Experiment at CERN

    Delonca, M; Grenier, D; Maglioni, C; Sarrio Martinez, A


    At CERN, the High Radiation to Materials facility (HiRadMat) is designed to test accelerator components under the impact of high-intensity pulsed beams and will start operation in 2012. In this frame an LHC TED-type dump was installed at the end of the line, working in nitrogen overpressure, and a 254μm-thick beryllium window was placed as barrier between the inside of the dump and the external atmosphere. Because of the special loading conditions, a clamped window design was especially developed, optimized and implemented, the more standard welded window not being suitable for such loads. Considering then the clamping force and the applied differential pressures, the stresses on the window components were carefully evaluated thanks to empirical as well as numerical models, to guarantee the structural integrity of the beryllium foil. This paper reports on choices and optimizations that led to the final design, presenting also comparative results from different solutions and the detailed results for the adopt...


    ZHOU Yunshan; LIU Jin'gang; CAI Yuanchun; ZOU Naiwei


    Associated dynamic performance of the clamping force control valve used in continuously variable transmission (CVT) is optimized. Firstly, the structure and working principle of the valve are analyzed, and then a dynamic model is set up by means of mechanism analysis. For the purpose of checking the validity of the modeling method, a prototype workpiece of the valve is manufactured for comparison test, and its simulation result follows the experimental result quite well. An associated performance index is founded considering the response time, overshoot and saving energy, and five structural parameters are selected to adjust for deriving the optimal associated performance index. The optimization problem is solved by the genetic algorithm (GA) with necessary constraints. Finally, the properties of the optimized valve are compared with those of the prototype workpiece, and the results prove that the dynamic performance indexes of the optimized valve are much better than those of the prototype workpiece.Key words: Dynamic modeling Optimal design Genetic algorithm Clamping force control valve Continuously variable transmission (CVT)

  1. Requirements to gap widths and clamping for CO2 laser butt welding

    Gong, Hui; Juhl, Thomas Winther


    In the experimental study of fixturing and gap width requirements a clamping device for laser butt welding of steel sheets has been developed and tested. It has fulfilled the work and made the gap width experiments possible.It has shown that the maximum allowable gap width to some extent is inver......In the experimental study of fixturing and gap width requirements a clamping device for laser butt welding of steel sheets has been developed and tested. It has fulfilled the work and made the gap width experiments possible.It has shown that the maximum allowable gap width to some extent...... is inversely related to the welding speed. Also larger laser power leads to bigger allowable gap widths. The focal point position, though, has little influence on the maximum allowable gap width.During analysis X-ray photos show no interior porosity in the weld seam. Other methods have been applied to measure...... responses from variations in welding parameters.The table below lists the results of the study, showing the maximum allowable gap widths and some corresponding welding parameters.Maximum allowable Gap Width; Welding Speed; Laser Power:0.10 mm2 m/min2, 2.6 kW0.15 mm1 m/min2 kW0.20 mm1 m/min2.6 kW0.30 mm0.5 m...

  2. Artificial neural networks based controller for glucose monitoring during clamp test.

    Merav Catalogna

    Full Text Available Insulin resistance (IR is one of the most widespread health problems in modern times. The gold standard for quantification of IR is the hyperinsulinemic-euglycemic glucose clamp technique. During the test, a regulated glucose infusion is delivered intravenously to maintain a constant blood glucose concentration. Current control algorithms for regulating this glucose infusion are based on feedback control. These models require frequent sampling of blood, and can only partly capture the complexity associated with regulation of glucose. Here we present an improved clamp control algorithm which is motivated by the stochastic nature of glucose kinetics, while using the minimal need in blood samples required for evaluation of IR. A glucose pump control algorithm, based on artificial neural networks model was developed. The system was trained with a data base collected from 62 rat model experiments, using a back-propagation Levenberg-Marquardt optimization. Genetic algorithm was used to optimize network topology and learning features. The predictive value of the proposed algorithm during the temporal period of interest was significantly improved relative to a feedback control applied at an equivalent low sampling interval. Robustness to noise analysis demonstrates the applicability of the algorithm in realistic situations.

  3. Automatic Calibration of Frequency Compensation System in Computer-Controlled Patch-Clamp Amplifier

    Jun Xion


    Full Text Available Computer-controlled patch-clamp amplifier is a digitally controlled analog device used to record the cellular ion channel currents in electrophysiology research. The inherent bandwidth and performance of the headstage is limited by the stray capacitance and distributed capacitance across the feedback resistors. In order to effectively improve the performance of the headstage, the paper advanced a simplified automatic calibration method of frequency compensation system in resistor-feedback patch-clamp amplifier. The dynamic model of headstage was approximate as a two poles and one zero system in the transfer function by experience and test results, so the dynamic characteristics of the headstage were obtained employing least squares parameter estimation algorithm. Further more, the compensation parameter of high frequency booster can be estimated by the time constant of main pole of headstage. And automatic adjustment of the parameters in transient response correction stage was performed as a least squares fitting problem. The software routine running on the host computer conducted all operations of frequency compensation. Experimental results demonstrate that the simplified automatic calibration method can substantially extend the bandwidth and minimize step response error of headstage.

  4. The use of clamping grips and friction pads by tree frogs for climbing curved surfaces

    Ji, Aihong; Yuan, Shanshan; Hill, Iain; Wang, Huan; Barnes, W. Jon P.; Dai, Zhendong; Sitti, Metin


    Most studies on the adhesive mechanisms of climbing animals have addressed attachment against flat surfaces, yet many animals can climb highly curved surfaces, like twigs and small branches. Here we investigated whether tree frogs use a clamping grip by recording the ground reaction forces on a cylindrical object with either a smooth or anti-adhesive, rough surface. Furthermore, we measured the contact area of fore and hindlimbs against differently sized transparent cylinders and the forces of individual pads and subarticular tubercles in restrained animals. Our study revealed that frogs use friction and normal forces of roughly a similar magnitude for holding on to cylindrical objects. When challenged with climbing a non-adhesive surface, the compressive forces between opposite legs nearly doubled, indicating a stronger clamping grip. In contrast to climbing flat surfaces, frogs increased the contact area on all limbs by engaging not just adhesive pads but also subarticular tubercles on curved surfaces. Our force measurements showed that tubercles can withstand larger shear stresses than pads. SEM images of tubercles revealed a similar structure to that of toe pads including the presence of nanopillars, though channels surrounding epithelial cells were less pronounced. The tubercles' smaller size, proximal location on the toes and shallow cells make them probably less prone to buckling and thus ideal for gripping curved surfaces. PMID:28228509

  5. Influence of squeeze film damping on the higher-order modes of clamped–clamped microbeams

    Alcheikh, N


    This paper presents an experimental study and a finite-element analysis of the effect of squeeze film damping on the resonance frequency and quality factor of the higher-order flexure vibrations modes of clamped-clamped microbeams. Viscoelastic and silicon nitride microbeams are fabricated and are electrostatically actuated by various electrode configurations to trigger the first, second, and third modes. The damping characteristic and the resonance frequency of these modes are examined for a wide range of gas pressure and electrostatic voltage loads. The results of the silicon nitride beams and viscoelastic beams are compared. It is found that the intrinsic material loss is the major dissipation mechanism at low pressure for the viscoelastic microbeams, significantly limiting their quality factor. It is also found that while the silicon nitride beams show higher quality factors at the intrinsic and molecular regimes of pressure, due to their low intrinsic loss, their quality factors near atmospheric pressure are lower than those of the viscoelastic microbeams. Further, the higher-order modes of all the beams show much higher quality factors at atmospheric pressure compared to the first mode, which could be promising for operating such resonators in air. Experimental results and finite element model simulations show good agreement for resonance frequency and quality factor for the three studied modes. © 2016 IOP Publishing Ltd.

  6. Potential clamp of isolated dialyzed neuron: minimalization of the effect of series resistance.

    Stolc, S; Nemcek, V; Boska, D


    A modification of the technique of intracellular dialysis of isolated single excitable cells, such as rat spinal ganglion neuron, suitable for potential clamping of its somatic membrane is described. The advantage of the new modification is the substantial reduction of the effect of inherent resistance in series (RS) to the membrane resistance (RM) on precision of potential clamping. This is attained by reversal of cell position in the perfusion pipette resulting in an approximately tenfold reduction in the area of active membrane. The resistance of this area proportionally increased while RS remained unchanged. Hence the error in potential fixation, which is inversely proportional to the ratio RM/RS, is by approximately one order smaller with the new technique than with the original one. An essential step in the new technique is the osmotic expansion of the cell to improve the contact of the cell with the perfusion pipette in the pore and to facilitate disruption of the appropriate part of the membrane. All features and advantages of the technique of intracellular dialysis, such as simplicity, the possibility to easily change ionic composition of media, and/or to apply drugs to any side of the membrane in the same cell, etc., have been maintained.

  7. Higher order modes excitation of electrostatically actuated clamped–clamped microbeams: experimental and analytical investigation

    Jaber, Nizar


    © 2016 IOP Publishing Ltd. In this study, we demonstrate analytically and experimentally the excitations of the higher order modes of vibrations in electrostatically actuated clamped-clamped microbeam resonators. The concept is based on using partial electrodes with shapes that induce strong excitation of the mode of interest. The devices are fabricated using polyimide as a structural layer coated with nickel from the top and chrome and gold layers from the bottom. Experimentally, frequency sweeps with different electro-dynamical loading conditions are shown to demonstrate the excitation of the higher order modes of vibration. Using a half electrode, the second mode is excited with high amplitude of vibration compared with almost zero response using the full electrode. Also, using a two-third electrode configuration is shown to amplify the third mode resonance amplitude compared with the full electrode under the same electrical loading conditions. An analytical model is developed based on the Euler-Bernollui beam model and the Galerkin method to simulate the device response. Good agreement between the simulation results and the experimental data is reported.

  8. Visually guided whole cell patch clamp of mouse supraoptic nucleus neurons in cultured and acute conditions.

    Stachniak, Tevye J E; Bourque, Charles W


    Recent advances in neuronal culturing techniques have supplied a new set of tools for studying neural tissue, providing effective means to study molecular aspects of regulatory elements in the supraoptic nucleus of the hypothalamus (SON). To combine molecular biology techniques with electrophysiological recording, we modified an organotypic culture protocol to permit transfection and whole cell patch-clamp recordings from SON cells. Neonatal mouse brain coronal sections containing the SON were dissected out, placed on a filter insert in culture medium, and incubated for at least 4 days to allow attachment to the insert. The SON was identifiable using gross anatomical landmarks, which remained intact throughout the culturing period. Immunohistochemical staining identified both vasopressinergic and oxytocinergic cells present in the cultures, typically appearing in well-defined clusters. Whole cell recordings from these cultures demonstrated that certain properties of the neonatal mouse SON were comparable to adult mouse magnocellular neurons. SON neurons in both neonatal cultures and acute adult slices showed similar sustained outward rectification above -60 mV and action potential broadening during evoked activity. Membrane potential, input resistance, and rapidly inactivating potassium current density (IA) were reduced in the cultures, whereas whole cell capacitance and spontaneous synaptic excitation were increased, perhaps reflecting developmental changes in cell physiology that warrant further study. The use of the outlined organotypic culturing procedures will allow the study of such electrophysiological properties of mouse SON using whole cell patch-clamp, in addition to various molecular, techniques that require longer incubation times.

  9. Indirect Field Oriented Control for Five Phase Three Level Neutral Point Clamped Inverter Fed PMSM Drive

    S. Sengottaian


    Full Text Available This study deals with a five phase three level Neutral Point Clamped (NPC inverter fed PMSM drive application. The motor performances depend upon mathematical model so the parameters vary are: noise, common mode voltage, flux variation and harmonic levels of the inverter or motor. Voltage saturation is one of the major problems of a motor which occurs due to speed oscillations, more current fluctuations. This problem can be solved by using PWM technique depends on the reference motor torque and flux. In this study Indirect Field Oriented Control (IFOC NPC inverter is suggested to reduce the voltage saturation. The three level neutral point clamped inverter is widely used for medium and high level applications. Compared with standard two level inverter, this type of NPC inverters have more merits. It generates greater number of levels output waveform in lower harmonic content at the same switching frequency and less voltage stress across the semiconductor switches; finally motor performance and control schemes are verified by using MATLAB/SIMULINK.

  10. A Molecular Imaging Approach to Mercury Sensing Based on Hyperpolarized (129)Xe Molecular Clamp Probe.

    Guo, Qianni; Zeng, Qingbin; Jiang, Weiping; Zhang, Xiaoxiao; Luo, Qing; Zhang, Xu; Bouchard, Louis-S; Liu, Maili; Zhou, Xin


    Mercury pollution, in the form of mercury ions (Hg(2+)), is a major health and environmental hazard. Commonly used sensors are invasive and limited to point measurements. Fluorescence-based sensors do not provide depth resolution needed to image spatial distributions. Herein we report a novel sensor capable of yielding spatial distributions by MRI using hyperpolarized (129)Xe. A molecular clamp probe was developed consisting of dipyrrolylquinoxaline (DPQ) derivatives and twocryptophane-A cages. The DPQ derivatives act as cation receptors whereas cryptophane-A acts as a suitable host molecule for xenon. When the DPQ moiety interacts with mercury ions, the molecular clamp closes on the ion. Due to overlap of the electron clouds of the two cryptophane-A cages, the shielding effect on the encapsulated Xe becomes important. This leads to an upfield change of the chemical shift of the encapsulated Xe. This sensor exhibits good selectivity and sensitivity toward the mercury ion. This mercury-activated hyperpolarized (129)Xe-based chemosensor is a new concept method for monitoring Hg(2+) ion distributions by MRI.

  11. Mechanically clamped PZT ceramics investigated by First-order reversal curves diagram

    Laurentiu Stoleriu


    Full Text Available The First Order Reversal Curves (FORC diagrams method was developed for characterizing the switching properties of ferroelectrics. In the present paper, the FORC method was applied for hard Pb(Zr,TiO3 ceramics with symmetric and asymmetric clamping. An ideal high-oriented single-crystalline ferroelectric with rectangular P(E loop would be characterised by a delta-function FORC distribution, while real ferroelectrics and mostly the polycrystalline ceramics show dispersed FORC distributions. All the investigated ceramics show FORC distributions with non-Gaussian shape, slightly elongated along the coercitive axis, meaning a high dispersion of the energy barriers separating the two bi-stable polarizations ±P. The degree of dispersion is enhanced by clamping. The maximum FORC coercivity is located at ~ (1.9-2 MV/m for all the hard ceramics. The FORC cycling experiment causes the reversal of the initial poling and result in a positive/negative bias on the FORC diagrams. According to the observed features, it results that FORC coercivity is more related to the nature of the material, while the bias field is more sensitive to the electrical and mechanical boundary conditions in which the ferroelectric ceramics evolves while switching.

  12. Ion channels as medicinal targets of biological toxins: the impact of automated patch-clamp electrophysiology.

    Picones, Arturo


    Patch-Clamp electrophysiology, the "gold standard" for the functional study of ion channels has become automated. This innovative technology, already over a decade old, has revolutionized the strategies for the search of medicinal compounds which now can be screened at unprecedented speed, approaching the high throughput standards required by primary screening campaigns emblematic of the pharmaceutical and biotechnology industries. Consequently, an acceleration of the discovery and development of new drugs targeting ion channels is expected. These pore forming membrane proteins had been relegated as crucial therapeutic drug targets due to the difficulty of their experimental analysis. This new technological approach has begun to impact the finding of new toxins which are conspicuously relevant as medicinal agents given their extraordinary potency and specificity when acting upon ion channels. The introduction of automated patch-clamp instrumentation to academic labs and institutions pursuing the finding of new pharmacological agents, peptide toxins in particular, will certainly enrich these scientific and technological fields by contributing with their always prolific generosity of originality and innovation.

  13. High Performance ZVT with Bus Clamping Modulation Technique for Single Phase Full Bridge Inverters

    Xia, Yinglai; Ayyanar, Raja


    This paper proposes a topology based on bus clamping modulation and zero-voltage-transition (ZVT) technique to realize zero-voltage-switching (ZVS) for all the main switches of the full bridge inverters, and inherent ZVS and/or ZCS for the auxiliary switches. The advantages of the strategy include significant reduction in the turn-on loss of the ZVT auxiliary switches which typically account for a major part of the total loss in other ZVT circuits, and reduction in the voltage ratings of auxiliary switches. The modulation scheme and the commutation stages are analyzed in detail. Finally, a 1kW, 500 kHz switching frequency inverter of the proposed topology using SiC MOSFETs has been built to validate the theoretical analysis. The ZVT with bus clamping modulation technique of fixed timing and adaptive timing schemes are implemented in DSP TMS320F28335 resulting in full ZVS for the main switches in the full bridge inverter. The proposed scheme can save up to 33 % of the switching loss compared with no ZVT case.

  14. Direct Torque Control for Three-Level Neutral Point Clamped Inverter-Fed Induction Motor Drive

    M. K. Sahu


    Full Text Available Direct torque control (DTC is a control technique in AC drive systems to obtain high performance torque control. The classical DTC drive contains a pair of hysteresis comparators and suffers from variable switching frequency and high torque ripple. These problems can be solved by using space vector depending on the reference torque and flux. In this paper the space vector modulation technique is applied to the three-level Neutral Point Clamped (NPC inverter control in the proposed DTC-based induction motor drive system, resulting to a significant reduce of torque ripple. Three-level neutral point clamped inverters have been widely used in medium voltage applications. This type of inverters have several advantages over standard two-level VSI, such as greater number of levels in the output voltage waveforms, less harmonic distortion in voltage and current waveforms and lower switching frequencies. This paper emphasizes the derivation of switching states using the Space Vector Pulse Width Modulation (SVPWM technique. The control scheme is implemented using Matlab/Simulink. Experimental results using dSPACE validate the steady-state and the dynamic performance of the proposed control strategy.

  15. Does clamping during liver surgery predispose to thrombosis of the hepatic veins? Analysis of 210 cases

    Nikolaos Arkadopoulos; Vaia Stafyla; Athanasios Marinis; Vassilios Koutoulidis; Kassiani Theodoraki; Theodosios Theodosopoulos; Ioannis Vassiliou; Nikolaos Dafnios; Georgios Fragulidis; Vassilios Smyrniotis


    AIM: To test whether clamping during liver surgery predisposes to hepatic vein thrombosis.METHODS: We performed a retrospective analysis of 210 patients who underwent liver resection with simultaneous inflow and outflow occlusion.Intraoperatively, flow in the hepatic veins was assessed by Doppler ultrasonography during the reperfusion phase. Postoperatively, patency of the hepatic veins was assessed by contrast-enhanced CT angiography,when necessary after 3-6 mo follow up.RESULTS: Twelve pat ients (5.7%) developed intraoperative liver remnant swelling. However,intraoperative ultrasonography did not reveal evidence of hepatic vein thrombosis. In three of these patients a kinking of the common trunk of the middle and left hepatic veins hindering outflow was recognized and was managed successfully by suturing the liver remnant to the diaphragm. Twenty three patients (10.9%) who developed signs of mild outflow obstruction postoperatively, had no evidence of thrombi in the hepatic veins or flow disturbances on ultrasonography and contrast-enhanced CT angiography, while hospitalized. Long term assessment of the patency of the hepatic veins over a 3-6 mo follow-up period did not reveal thrombi formation or clinical manifestations of outflow obstruction.CONCLUSION: Extrahepatic dissection and clamping of the hepatic veins does not predispose to clinically important thrombosis.

  16. Downhole pulse radar

    Chang, Hsi-Tien


    A borehole logging tool generates a fast rise-time, short duration, high peak-power radar pulse having broad energy distribution between 30 MHz and 300 MHz through a directional transmitting and receiving antennas having barium titanate in the electromagnetically active region to reduce the wavelength to within an order of magnitude of the diameter of the antenna. Radar returns from geological discontinuities are sampled for transmission uphole. 7 figs.

  17. Capacitance of Ti3C2Tx MXene in ionic liquid electrolyte

    Lin, Zifeng; Barbara, Daffos; Taberna, Pierre-Louis; Van Aken, Katherine L.; Anasori, Babak; Gogotsi, Yury; Simon, Patrice


    Ti3C2Tx MXene, a two-dimensional (2D) early transition metal carbide, has shown an extremely high volumetric capacitance in aqueous electrolytes, but in a narrow voltage window (less than 1.23 V). The utilization of MXene materials in ionic liquid electrolytes with a large voltage window has never been addressed. Here, we report the preparation of the Ti3C2Tx MXene ionogel film by vacuum filtration for use as supercapacitor electrodes operating in 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (EMI-TFSI) neat ionic liquid electrolyte. Due to the disordered structure of the Ti3C2Tx hydrogel film and a stable spacing after vacuum drying, achieved through ionic liquid electrolyte immersion of the Ti3C2Tx hydrogel film, the Ti3C2Tx surface became accessible to EMI+ and TFSI- ions. A capacitance of 70 F g-1 together with a large voltage window of 3 V was obtained at a scan rate of 20 mV s-1 in neat EMI-TFSI electrolyte. The electrochemical signature indicates a capacitive behavior even at a high scan rate (500 mV s-1) and a high power performance. This work opens up the possibilities of using MXene materials with various ionic liquid electrolytes.

  18. Path-integral molecular dynamics simulation of 3C-SiC

    Ramírez, Rafael; Herrero, Carlos P.; Hernández, Eduardo R.; Cardona, Manuel


    Molecular dynamics simulations of 3C-SiC have been performed as a function of pressure and temperature. These simulations treat both electrons and atomic nuclei by quantum mechanical methods. While the electronic structure of the solid is described by an efficient tight-binding Hamiltonian, the nuclei dynamics is treated by the path-integral formulation of statistical mechanics. To assess the relevance of nuclear quantum effects, the results of quantum simulations are compared to others where either the Si nuclei, the C nuclei, or both atomic nuclei are treated as classical particles. We find that the experimental thermal expansion of 3C-SiC is realistically reproduced by our simulations. The calculated bulk modulus of 3C-SiC and its pressure derivative at room temperature show also good agreement with the available experimental data. The effect of the electron-phonon interaction on the direct electronic gap of 3C-SiC has been calculated as a function of temperature and related to results obtained for bulk diamond and Si. Comparison to available experimental data shows satisfactory agreement, although we observe that the employed tight-binding model tends to overestimate the magnitude of the electron-phonon interaction. The effect of treating the atomic nuclei as classical particles on the direct gap of 3C-SiC has been assessed. We find that nonlinear quantum effects related to the atomic masses are particularly relevant at temperatures below 250K .

  19. Nonacarbonyl-1κ3C,2κ3C,3κ3C-μ-bis(diphenylarsinomethane-1:2κ2As:As'-[tris(2-chloroethyl phosphite-3κP]-triangulo-triruthenium(0

    Omar bin Shawkataly


    Full Text Available In the title triangulo-triruthenium(0 compound, [Ru3(C25H22As2(C6H12Cl3O3P(CO9], the bis(diphenylarsinomethane ligand bridges an Ru—Ru bond and the monodentate phosphine ligand bonds to the third Ru atom. Both the arsine and phosphine ligands are equatorial with respect to the Ru3 triangle. In addition, each Ru atom carries one equatorial and two axial terminal carbonyl ligands. In the crystal packing, the molecules are linked by intermolecular C—H...O hydrogen bonds into a three-dimensional framework. Weak intermolecular C—H...π interactions further stabilize the crystal structure.

  20. TEM Method for Identification of Domains in Materials which Undergo a Pm3m3->R3c or R3c Phase Transition

    Bilde-Sørensen, Jørgen


    The c-axis of the domains formed by a Pm3m→R3c or R3¯c phase transition may lie along any of the four 〈111〉 C directions of the cubic phase. It is shown that the orientation of the c-axis can be determined by TEM by examining whether two suitable 1/3{113}C superlattice reflections are allowed...... or forbidden in the trigonal phase. As an example, the method is used to examine domains in LaAlO3...

  1. Lowest instrumented vertebra selection in Lenke 3C and 6C scoliosis

    Wang, Yu; Bünger, Cody; Zhang, Yanqun;


    PURPOSE: The aim of this study was to investigate whether or not post-op curve behaviour differs due to different choices of lowest instrumented vertebra (LIV) with reference to lumbar apical vertebra (LAV) in Lenke 3C and 6C scoliosis. METHODS: We reviewed all the AIS cases surgically treated...... in our institution from 2002 through 2008. Inclusion criteria were as follows: (1) patients with Lenke 3C or 6C scoliosis who were treated with posterior pedicle screw-only constructs; (2) 2-year radiographic follow-up. All the included patients were categorized into three groups based on the relative...... surgery. No significant differences were found in thoracic or lumbar correction rate, global coronal balance and incidence rate of trunk shift among the three groups. CONCLUSION: In conclusion, in Lenke 3C and 6C scoliosis, post-op lumbar curve behaviour differs due to different choices of LIV...


    P. V. Bulat


    Full Text Available The paper deals with creation of heteropolytypes instrument structures on silicon carbide for power devices not subjected to degradation of electrical properties. The phenomenon of polytypism is considered. Characteristics of different SiC polytypes are given. Information is cited about the causes and effects for degradation of p-n -structures of power devices based on SiC at large density direct current passing. It is shown that hetero-transitions between SiC polytypes may have more structural perfection than hetero-transitions between semiconductors with different chemical nature. Conclusion is made about application prospects for heterostructures based on 3C-SiC polytype in devices of modern power electronics. A brief overview of the possible methods for 3C-SiC single crystals growth is done. A basic scheme for creation of heteropolytype 3C-SiC structures based on substrates of 6H-SiC polytype is suggested.

  3. Cr3C2及(W,Ti)C对Al2O3/Cr3 C2/(W,Ti)C复合陶瓷材料Vickers硬度的影响%Effect of Cr3C2 and (W,Ti)C on the Vickers Hardness of Al2O3/Cr3 C2/(W, Ti) C Ceramic Composites

    孙德明; 鹿晓阳; 李成美; 薛仲舜; 陈怀明; 岳雪涛; 孙胜



  4. Kinetic and thermodynamics studies on the decompositions of Ni{sub 3}C in different atmospheres

    Leng Yonghua; Xie Lei; Liao Fuhui; Zheng Jie [Beijing National Laboratory for Molecular Sciences (BNLMS), State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China); Li Xingguo [Beijing National Laboratory for Molecular Sciences (BNLMS), State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 (China); College of Engineering, Peking University, Beijing 100871 (China)], E-mail:


    The thermal decompositions (including TG and DSC) of nickel carbide were studied under different atmospheres of Ar, air and H{sub 2}. X-ray diffraction combined with element analysis indicated that nickel metal, together with solid amorphous carbon, was formed during Ni{sub 3}C decomposition in Ar atmosphere, accompanying mass invariant in this process. While in H{sub 2} atmosphere nickel metal was the only residual from reactions. The carbon component of nickel carbide reacted with H{sub 2} to form methane as the main volatile gases. Both the nickel and carbon components of Ni{sub 3}C reacted with O{sub 2} in the air to form their corresponding oxides. Moreover, we calculated the activation energy for the decomposition process and the molar enthalpy of formation of Ni{sub 3}C based on the thermal analysis.

  5. Spitzer and near-infrared observations of the young supernova remnant 3C397

    Rho, Jeonghee; Jarrett, Tom


    We present Spitzer IRS, IRAC and MIPS observations and near-infrared imaging and spectroscopy of the young supernova remnant 3C397 (G41.1-0.2). Near-infrared observations were made using the Palomar 200 inch telescope. Both mid- and near-infrared spectra are dominated by Fe lines and near-infrared imaging shows bright Fe emission with a shell-like morphology. There is no molecular hydrogen line belong to the SNR and some are in background. The Ni, Ar, S and Si lines are detected using IRS and hydrogen recombination lines are detected in near-infrared. Two nickel lines at 18.24 and 10.69 micron are detected. 3C397 is ejecta-dominated, and our observations support 3C397 to be a Type Ia supernova.

  6. U-Boot在S3C2440上移植和设置%Porting and Setting Method of U-Boot on S3C2440 Platform

    王之磊; 李临生


    BootLoader是嵌入式系统开发的重要环节,是操作系统和硬件平台联系的桥梁,对嵌入式系统的后继开发十分重要.简介了BootLoader U-Boot目录结构、工作流程及其移植的思路和引导内核的方法;给出了移植uboot-2010-03到ARM微处理器S3C2440上,所需操作环境的搭建、具体代码的修改、具体参数的设置、所需的操作命令及常见错误的处理方法等要点.最后将编译生成了U-Boot.bin文件下载到ARM微处理器S3C2440上,经过测试U-Boot可以达到设计的功能要求并能够稳定的运行而且通过对其正确的配置成功的引导了内核.

  7. Porting of Linux kernel system in S3C2410 board%Linux内核在S3C2410上移植的研究



    To further explore the basic technology of building the embedded Linux system, an approach of porting Linux 2. 4. 18. -rm7-pxal to the board based on S3C2410 processor is described. The method and process of porting Linux kernel are described in detail. The method how to build a porting environment is illustrated. The running result shws that the method is simple and practical, and can reach the scheduled goal.%以研究将Linux内核移植到嵌入式系统的技术为目的,描述了Linux-2.4.18.-rm7-pxa1移植到S3C2410处理器目标板上的方法.详细介绍了Linux内核移植方法与步骤,并说明了如何搭建移植环境.结果表明,该方法简单实用,达到了预定目标.

  8. Synthesis of WC powder through microwave heating of WO3-C mixture

    Behnami, Amir Karimzadeh; Hoseinpur, Arman; Sakaki, Masoud; Bafghi, Mohammad Sh.; Yanagisawa, Kazumichi


    A simple, easy, and low-cost process for the fabrication of tungsten carbide (WC) powder through microwave heating of WO3-C mixtures was developed. Thermodynamic calculations and experimental investigations were carried out for WO3-C and W-C systems, and a formation mechanism was proposed. In the results, for the synthesis of WC, the use of over stoichiometric amount of C together with a specially assembled experimental setup (which effectively retains heat in the system) is necessary. The WC powder is successfully obtained by heating WO3:5C mixture for 900 s in a domestic microwave oven.

  9. Model Research On Synthesis Of Al2O3-C Layers By MOCVD

    Sawka A.


    Full Text Available These are model studies whose aim is to obtain information that would allow development of new technology for synthesizing monolayers of Al2O3-C with adjusted microstructure on cemented carbides. The Al2O3-C layer will constitute an intermediate layer on which the outer layer of Al2O3 without carbon is synthesized. The purpose of the intermediate layer is to block the cobalt diffusion to the synthesized outer layer of Al2O3 and to stop the diffusion of air oxygen to the substrate during the synthesis of the outer layer. This layer should be thin, continuous, dense and uniform in thickness.

  10. Polymorphism control of superconductivity and magnetism in Cs(3)C(60) close to the Mott transition.

    Ganin, Alexey Y; Takabayashi, Yasuhiro; Jeglic, Peter; Arcon, Denis; Potocnik, Anton; Baker, Peter J; Ohishi, Yasuo; McDonald, Martin T; Tzirakis, Manolis D; McLennan, Alec; Darling, George R; Takata, Masaki; Rosseinsky, Matthew J; Prassides, Kosmas


    The crystal structure of a solid controls the interactions between the electronically active units and thus its electronic properties. In the high-temperature superconducting copper oxides, only one spatial arrangement of the electronically active Cu(2+) units-a two-dimensional square lattice-is available to study the competition between the cooperative electronic states of magnetic order and superconductivity. Crystals of the spherical molecular C(60)(3-) anion support both superconductivity and magnetism but can consist of fundamentally distinct three-dimensional arrangements of the anions. Superconductivity in the A(3)C(60) (A = alkali metal) fullerides has been exclusively associated with face-centred cubic (f.c.c.) packing of C(60)(3-) (refs 2, 3), but recently the most expanded (and thus having the highest superconducting transition temperature, T(c); ref. 4) composition Cs(3)C(60) has been isolated as a body-centred cubic (b.c.c.) packing, which supports both superconductivity and magnetic order. Here we isolate the f.c.c. polymorph of Cs(3)C(60) to show how the spatial arrangement of the electronically active units controls the competing superconducting and magnetic electronic ground states. Unlike all the other f.c.c. A(3)C(60) fullerides, f.c.c. Cs(3)C(60) is not a superconductor but a magnetic insulator at ambient pressure, and becomes superconducting under pressure. The magnetic ordering occurs at an order of magnitude lower temperature in the geometrically frustrated f.c.c. polymorph (Néel temperature T(N) = 2.2 K) than in the b.c.c.-based packing (T(N) = 46 K). The different lattice packings of C(60)(3-) change T(c) from 38 K in b.c.c. Cs(3)C(60) to 35 K in f.c.c. Cs(3)C(60) (the highest found in the f.c.c. A(3)C(60) family). The existence of two superconducting packings of the same electronically active unit reveals that T(c) scales universally in a structure-independent dome-like relationship with proximity to the Mott metal-insulator transition

  11. Preparation and Photoemission Spectra of Rb3C60 Single-Crystal Thin Films

    李宏年; 吴太权; 陈晓; 李海洋; 鲍世宁; 徐亚伯; 钱海杰; 易卜拉欣奎热西; 刘风琴


    Rb3 C60 single-crystal thin films were prepared on the cleaved (111) surface of C60 single crystal. The photoemission spectrum line shapes of the lowest unoccupied molecular orbital (LUMO) derived band at room temperature and 150K were established by synchrotron radiation photoemission spectrum measurements. The density of states near the Fermi level was distinctly affected by temperature. No less than six sub-peaks of the LUMOband were observed even at room temperature. The existence of so many sub-peaks offered the opportunities to analyse in more detail the orientational structure and the electron-Boson interactions of the narrow-band metallic Rb3 C60 .

  12. Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin.

    Lei Wen

    Full Text Available BACKGROUND: Optogenetic manipulation of a neuronal network enables one to reveal how high-order functions emerge in the central nervous system. One of the Chlamydomonas rhodopsins, channelrhodopsin-1 (ChR1, has several advantages over channelrhodopsin-2 (ChR2 in terms of the photocurrent kinetics. Improved temporal resolution would be expected by the optogenetics using the ChR1 variants with enhanced photocurrents. METHODOLOGY/PRINCIPAL FINDINGS: The photocurrent retardation of ChR1 was overcome by exchanging the sixth helix domain with its counterpart in ChR2 producing Channelrhodopsin-green receiver (ChRGR with further reform of the molecule. When the ChRGR photocurrent was measured from the expressing HEK293 cells under whole-cell patch clamp, it was preferentially activated by green light and has fast kinetics with minimal desensitization. With its kinetic advantages the use of ChRGR would enable one to inject a current into a neuron by the time course as predicted by the intensity of the shedding light (opto-current clamp. The ChRGR was also expressed in the motor cortical neurons of a mouse using Sindbis pseudovirion vectors. When an oscillatory LED light signal was applied sweeping through frequencies, it robustly evoked action potentials synchronized to the oscillatory light at 5-10 Hz in layer 5 pyramidal cells in the cortical slice. The ChRGR-expressing neurons were also driven in vivo with monitoring local field potentials (LFPs and the time-frequency energy distribution of the light-evoked response was investigated using wavelet analysis. The oscillatory light enhanced both the in-phase and out-phase responses of LFP at the preferential frequencies of 5-10 Hz. The spread of activity was evidenced by the fact that there were many c-Fos-immunoreactive neurons that were negative for ChRGR in a region of the motor cortex. CONCLUSIONS/SIGNIFICANCE: The opto-current-clamp study suggests that the depolarization of a small number of neurons

  13. Comparison: Simulation and Experimental Characterisation of an all-Optical Gain-Clamped Erbium-Doped Fibre Amplifier

    T. Subramaniam M. A. Mahdi P. Poopalan, and H. Ahmad


    Full Text Available This paper presents the GC-EDFA characteristics comparison between the simulated results (using EDFA_Design software and experimental results. The comparisons reveal the usefulness of the software in simulating the behaviour of an all-optical GC-EDFA system. Comparisons are made for values obtained from the system operated at high laser power, in order to highlight the differences between the experimental and simulated values. The main objective for this comparison is to prove the capability of the software in simulating the gain-clamped system. Therefore, the software can be used to test new configurations, aimed at improvising current gain-clamped EDFA performances.Keywords optical, gain-clamping, erbium, fibre amplifier, simulation, experiment

  14. Negligible substrate clamping effect on piezoelectric response in (111)-epitaxial tetragonal Pb(Zr, Ti)O{sub 3} films

    Yamada, Tomoaki, E-mail: [Department of Materials, Physics and Energy Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan); PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012 (Japan); Yasumoto, Jun; Ito, Daisuke; Yoshino, Masahito; Nagasaki, Takanori [Department of Materials, Physics and Energy Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan); Sakata, Osami [Synchrotron X-ray Station at SPring-8 and Synchrotron X-ray Group, National Institute for Materials Science, Sayo-cho, Sayo-gun, Hyogo 679-5148 (Japan); Department of Innovative and Engineered Material, Tokyo Institute of Technology, Yokohama 226-8503 (Japan); Imai, Yasuhiko [Japan Synchrotron Radiation Research Institute, SPring-8, Sayo-cho, Sayo-gun, Hyogo 679-5198 (Japan); Kiguchi, Takanori [Institute for Materials Research, Tohoku University, Sendai, Miyagi 980-8577 (Japan); Shiraishi, Takahisa; Shimizu, Takao; Funakubo, Hiroshi [Department of Innovative and Engineered Material, Tokyo Institute of Technology, Yokohama 226-8503 (Japan)


    The converse piezoelectric responses of (111)- and (001)-epitaxial tetragonal Pb(Zr{sub 0.35}Ti{sub 0.65})O{sub 3} [PZT] films were compared to investigate the orientation dependence of the substrate clamping effect. Synchrotron X-ray diffraction (XRD) and piezoelectric force microscopy revealed that the as-grown (111)-PZT film has a polydomain structure with normal twin boundaries that are changed by the poling process to inclined boundaries, as predicted by Romanov et al. [Phys. Status Solidi A 172, 225 (1999)]. Time-resolved synchrotron XRD under bias voltage showed the negligible impact of substrate clamping on the piezoelectric response in the (111)-PZT film, unlike the case for (001)-PZT film. The origin of the negligible clamping effect in the (111)-PZT film is discussed from the viewpoint of the elastic properties and the compensation of lattice distortion between neighboring domains.

  15. Robust Clamping Force Control of an Electro-Mechanical Brake System for Application to Commercial City Buses

    Sangjune Eum


    Full Text Available This paper proposes a sensor-less robust force control method for improving the control performance of an electro-mechanical brake (EMB which is applicable to commercial city buses. The EMB generates the accurate clamping force commanded by a driver through an independent motor control at each wheel instead of using existing mechanical components. In general, an EMB undergoes parameter variation and a backdrivability problem. For this reason, the cascade control strategy (e.g., force-position cascade control structure is proposed and the disturbance observer is employed to enhance control robustness against model variations. Additionally, this paper proposed the clamping force estimation method for a sensor-less control, i.e., the clamping force observer (CFO. Finally, in order to confirm the performance and effectiveness of a proposed robust control method, several experiments are performed and analyzed.

  16. Testing of New Materials and Computer Aided Optimization of Process Parameters and Clamping Device During Predevelopment of Laser Welding Processes

    Weidinger, Peter; Günther, Kay; Fitzel, Martin; Logvinov, Ruslan; Ilin, Alexander; Ploshikhin, Vasily; Hugger, Florian; Mann, Vincent; Roth, Stephan; Schmidt, Michael

    The necessity for weight reduction in motor vehicles in order to save fuel consumption pushes automotive suppliers to use materials of higher strength. Due to their excellent crash behavior high strength steels are increasingly applied in various structures. In this paper some predevelopment steps for a material change from a micro alloyed to dual phase and complex phase steels of a T-joint assembly are displayed. Initially the general weldability of the materials regarding pore formation, hardening in the heat affected zone and hot cracking susceptibility is discussed. After this basic investigation, the computer aided design optimization of a clamping device is shown, in which influences of the clamping jaw, the welding position and the clamping forces upon weld quality are presented. Finally experimental results of the welding process are displayed, which validate the numerical simulation.

  17. Firing clamp: A novel method for single-trial estimation of excitatory and inhibitory synaptic neuronal conductances

    Anton eChizhov


    Full Text Available Understanding non-stationary neuronal activity as seen in vivo requires estimation of both excitatory and inhibitory synaptic conductances from a single trial of recording. We propose a new intracellular recording method for this purpose called firing clamp. Synaptic conductances are estimated from the characteristics of artificially evoked probe spikes, namely the spike amplitude and the mean subthreshold potential, which are sensitive to both excitatory and inhibitory synaptic input signals. The probe spikes, timed at a fixed rate, are evoked in the dynamic-clamp mode by injected meander-like current steps, with the step duration depending on neuronal membrane voltage. We test the method with perforated-patch recordings from isolated cells stimulated by external application or synaptic release of transmitter, and validate the method with simulations of a biophysically-detailed neuron model. The results are compared with the conductance estimates based on conventional current-clamp recordings.

  18. Surgical site wound infection in relation to antibiotic prophylaxis given before skin incision and after cord clamping during cesarean delivery.

    Shrestha, B; Marhatha, R; Giri, A; Jaisi, S; Maskey, U


    Surgical site infection is one of the most common complications following Lower Segment Cesarean Section, which accounts for prolonged hospital stay thereby increasing expense. Prophylactic antibiotics in cesarean section reduces surgical site infection significantly. The best protection is provided when tissue level of antibiotics are adequate before incision, without prejudice to neonatal infectious morbidity. The objective of this study was to compare the incidence of surgical site wound infection with prophylactic antibiotics given before skin incision and after cord clamping following delivery of baby. This was a prospective, hospital based study, in which hundred cases of cesarean deliveries who received antibiotics prophylaxis one hour before the skin incision were compared with another 100 cases where antibiotic was given after cord clamping following delivery of the baby. Surgical site infection occurred in 3% of women who received antibiotics prophylaxis before skin incision as compared to 6% in whom antibiotic was given after cord clamping. It was statistically not significant (p = 0.465).

  19. Research and development of a self-centering clamping device for deep-water multifunctional pipeline repair machinery

    Liquan Wang


    Full Text Available When multifunctional pipeline repair machinery (MPRM is used in the deep sea area, it is difficult to grip the pipeline and ensure concentricity between the cutter heads and the pipeline during its operation. In view of this, a new system of two-arm holding self-centering pipeline clamping device was proposed. The system is composed of two groups of parallelogram double-rocker mechanism and cranking block mechanism which are symmetrically distributed on the frame. The geometric parameter solutions of the clamping device were analyzed with motion and transmission as the constraints. A mechanical model was established to associate the friction torque of clamping points with the driving force. Clamping device and machinery were designed and manufactured for the Ø304.8–457.2 mm pipelines used in this test. ADAMS simulation experiments were conducted underwater, and the cutting and beveling tests were carried out onshore. The following results are achieved. First, the smaller the pipe diameter, the smaller the transmission angle of the oscillating slider mechanism; the longer the hydraulic cylinder stroke, the greater the transmission angle of the double rocker mechanism. Second, the driving force of the clamping device increases with the increase of the pipe diameter. When the diameter reaches 457.2 mm, the hydraulic cylinder driving force of the clamping device should be greater than 10219 N. Third, the feed rate of the cutters increases suddenly due to the slight shaking of the machinery which occurs at the beginning of the pipe cutting, so it is necessary to adopt a small feed rate. And fourth, onshore experiment results agree well with the theoretical design and simulation results, proving the rationality of the system. The research results in this paper provide technical basis for the research and development of similar engineering prototypes.

  20. State-of-the-art automated patch clamp devices: Heat activation, action potentials and high throughput in ion channel screening

    Sonja eStoelzle


    Full Text Available Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. Diverse automated patch clamp systems with high-throughput capabilities are available for drug screening, but there are limitations in the application range. For example, solution exchange time, temperature control and the availability of the current clamp mode can be limiting factors. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. Here we introduce new features and platforms that meet the needs of drug discovery researchers and basic researchers alike.The Patchliner is an automated patch clamp system capable of recording up to 8 cells simultaneously with high success rates. Novel features such as temperature control and recordings in the current clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage clamp and current clamp modes with the view to finding new drug candidates and safety testing methods in a more physiologically relevant environment. The SyncroPatch 96, is a screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The system is an ideal tool for secondary screening efforts and for safety testing on ligand- and voltage-gated ion channels.The Patchliner and SyncroPatch 96 are ideal platforms for drug discovery, ion channel research and safety testing, combining long awaited features such as parallel action potential recordings and temperature control with extensively increased throughput.

  1. ATP binding and hydrolysis-driven rate-determining events in the RFC-catalyzed PCNA clamp loading reaction.

    Sakato, Miho; Zhou, Yayan; Hingorani, Manju M


    The multi-subunit replication factor C (RFC) complex loads circular proliferating cell nuclear antigen (PCNA) clamps onto DNA where they serve as mobile tethers for polymerases and coordinate the functions of many other DNA metabolic proteins. The clamp loading reaction is complex, involving multiple components (RFC, PCNA, DNA, and ATP) and events (minimally: PCNA opening/closing, DNA binding/release, and ATP binding/hydrolysis) that yield a topologically linked clamp·DNA product in less than a second. Here, we report pre-steady-state measurements of several steps in the reaction catalyzed by Saccharomyces cerevisiae RFC and present a comprehensive kinetic model based on global analysis of the data. Highlights of the reaction mechanism are that ATP binding to RFC initiates slow activation of the clamp loader, enabling it to open PCNA (at ~2 s(-1)) and bind primer-template DNA (ptDNA). Rapid binding of ptDNA leads to formation of the RFC·ATP·PCNA(open)·ptDNA complex, which catalyzes a burst of ATP hydrolysis. Another slow step in the reaction follows ATP hydrolysis and is associated with PCNA closure around ptDNA (8 s(-1)). Dissociation of PCNA·ptDNA from RFC leads to catalytic turnover. We propose that these early and late rate-determining events are intramolecular conformational changes in RFC and PCNA that control clamp opening and closure, and that ATP binding and hydrolysis switch RFC between conformations with high and low affinities, respectively, for open PCNA and ptDNA, and thus bookend the clamp loading reaction.

  2. Mycobacterium tuberculosis class II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III exhibits DNA regulated modes of interaction with the sliding DNA β-clamp.

    Khanam, Taran; Rai, Niyati; Ramachandran, Ravishankar


    The class-II AP-endonuclease (XthA) acts on abasic sites of damaged DNA in bacterial base excision repair. We identified that the sliding DNA β-clamp forms in vivo and in vitro complexes with XthA in Mycobacterium tuberculosis. A novel 239 QLRFPKK245 motif in the DNA-binding domain of XthA was found to be important for the interactions. Likewise, the peptide binding-groove (PBG) and the C-terminal of β-clamp located on different domains interact with XthA. The β-clamp-XthA complex can be disrupted by clamp binding peptides and also by a specific bacterial clamp inhibitor that binds at the PBG. We also identified that β-clamp stimulates the activities of XthA primarily by increasing its affinity for the substrate and its processivity. Additionally, loading of the β-clamp onto DNA is required for activity stimulation. A reduction in XthA activity stimulation was observed in the presence of β-clamp binding peptides supporting that direct interactions between the proteins are necessary to cause stimulation. Finally, we found that in the absence of DNA, the PBG located on the second domain of the β-clamp is important for interactions with XthA, while the C-terminal domain predominantly mediates functional interactions in the substrate's presence.

  3. Harmonic Mitigated Front End Three Level Diode Clamped High Frequency Link Inverter by Using MCI Technique

    Sreedhar Madichetty


    Full Text Available In this paper it proposes a high efficient soft-switching scheme based on zero-voltage-switching (ZVS and zero-current-switching(ZCS principle operated with a simple auxiliary circuit extended range for the front-end isolated DC-AC-DC-AC high power converter with an three phase three level diode clamped multi level inverter by using Minority Charge Carrier inspired optimization technique (MCI with Total Harmonic Distortion(THD,Switching losses, Selective harmonic elimination maintaining with its fundamental as an objective function. Input to the inverter is obtained by the photo voltaic cells and with battery bank. The switching scheme is optimized by MCI technique, analyzed and executed in Matlab and implemented with a digital signal processor (DSP .Experimental results with different loads have observed and shows its effectives, robustness of the applied technique.

  4. Force-clamp analysis techniques reveal stretched exponential unfolding kinetics in ubiquitin

    Lannon, Herbert; Brujic, Jasna


    Force-clamp spectroscopy reveals the unfolding and disulfide bond rupture times of single protein molecules as a function of the stretching force, point mutations and solvent conditions. The statistics of these times reveal whether the protein domains are independent of one another, the mechanical hierarchy in the polyprotein chain, and the functional form of the probability distribution from which they originate. It is therefore important to use robust statistical tests to decipher the correct theoretical model underlying the process. Here we develop multiple techniques to compare the well-established experimental data set on ubiquitin with existing theoretical models as a case study. We show that robustness against filtering, agreement with a maximum likelihood function that takes into account experimental artifacts, the Kuiper statistic test and alignment with synthetic data all identify the Weibull or stretched exponential distribution as the best fitting model. Our results are inconsistent with recently ...

  5. Acute hypotension induced by aortic clamp vs. PTH provokes distinct proximal tubule Na+ transporter redistribution patterns

    Leong, Patrick K K; Yang, Li E; Lin, Harrison W


    clearance. There was, however, no significant change in glomerular filtration rate (GFR) or subcellular distribution of NHE3 and NaPi2. In contrast, high-dose PTH rapidly (blood pressure to 51 +/- 3 mmHg, decreased urine output, and shifted NHE3 and NaPi2 out of the low......-density membranes enriched in apical markers. PTH at much lower doses (blood pressure and was diuretic. In conclusion, acute hypotension per se increases proximal tubule Na(+) reabsorption without changing NHE3 or NaPi2 subcellular distribution, indicating that trafficking...... in renal cortical membranes fractionated on sorbitol density gradients. Aortic clamp-induced acute hypotension (from 100 +/- 3 to 78 +/- 2 mmHg) provoked a 62% decrease in urine output and a significant decrease in volume flow from the proximal tubule detected as a 66% decrease in endogenous lithium...

  6. Global optimisation methods for poroelastic material characterisation using a clamped sample in a Kundt tube setup

    Vanhuyse, Johan; Deckers, Elke; Jonckheere, Stijn; Pluymers, Bert; Desmet, Wim


    The Biot theory is commonly used for the simulation of the vibro-acoustic behaviour of poroelastic materials. However, it relies on a number of material parameters. These can be hard to characterize and require dedicated measurement setups, yielding a time-consuming and costly characterisation. This paper presents a characterisation method which is able to identify all material parameters using only an impedance tube. The method relies on the assumption that the sample is clamped within the tube, that the shear wave is excited and that the acoustic field is no longer one-dimensional. This paper numerically shows the potential of the developed method. It therefore performs a sensitivity analysis of the quantification parameters, i.e. reflection coefficients and relative pressures, and a parameter estimation using global optimisation methods. A 3-step procedure is developed and validated. It is shown that even in the presence of numerically simulated noise this procedure leads to a robust parameter estimation.

  7. Molecular force spectroscopy with a DNA origami-based nanoscopic force clamp.

    Nickels, Philipp C; Wünsch, Bettina; Holzmeister, Phil; Bae, Wooli; Kneer, Luisa M; Grohmann, Dina; Tinnefeld, Philip; Liedl, Tim


    Forces in biological systems are typically investigated at the single-molecule level with atomic force microscopy or optical and magnetic tweezers, but these techniques suffer from limited data throughput and their requirement for a physical connection to the macroscopic world. We introduce a self-assembled nanoscopic force clamp built from DNA that operates autonomously and allows massive parallelization. Single-stranded DNA sections of an origami structure acted as entropic springs and exerted controlled tension in the low piconewton range on a molecular system, whose conformational transitions were monitored by single-molecule Förster resonance energy transfer. We used the conformer switching of a Holliday junction as a benchmark and studied the TATA-binding protein-induced bending of a DNA duplex under tension. The observed suppression of bending above 10 piconewtons provides further evidence of mechanosensitivity in gene regulation.

  8. The fluorescent detection of Paradiplozoon sp. (Monogenea: Diplozoidae attachment clamps' sclerites and integumental proteins : research communication

    S.J. Milne


    Full Text Available In the genus Paradiplozoon, few hard structures are present therefore most of the taxonomic studies have focused on the unique ultrastructure of the sclerites. Alcohol-fixed specimens were transferred to BSA-saline for 5 min. before staining indefinitely with 5 µl WGA-TRX, 5 µl SYTO 9 and 5 µl of Cal co fluor White M2R. Rhodamine, Fluorescein and DAPI bandpass filters on the microscope enabled selective light wavelength illumination of the three flourochromes by a mercury light source. This method provided an easy and rapid methodology to show the internal sclerites of attachment clamps. It is suitable for alcohol preserved specimens and may have additional applications in other helminth organisms.

  9. Endotoxemia-induced lymphocyte apoptosis is augmented by a hyperinsulinemic-euglycemic clamp

    Nielsen, Jeppe Sylvest; A, Larsson; Brix-Christensen, Vibeke


    BACKGROUND: Sepsis and endotoxemia are associated with lymphocyte apoptosis. This has been regarded as harmful, contributing to further immune suppression in already immune-compromised patients. Because normalization of blood glucose improves outcome in critically ill patients, the authors...... hypothesized that one of the effects of insulin and normoglycemia would be inhibition of lymphocyte apoptosis. Therefore, in this experimental study in pigs, the authors examined the separate and combined effects of acute endotoxemia and a hyperinsulinemic-euglycemic clamp (HEC) on lymphocyte apoptosis...... sections of each sample, the apoptosis of B and T lymphocytes were analyzed using stereologic methods: The number of apoptotic B and T cells was estimated by fluorescence immunohistochemistry with anti-active caspase-3 and either anti-CD21 (B lymphocytes) or anti-CD3epsilon (T lymphocytes). The number...

  10. Ultra-coherent nanomechanical resonators via soft clamping and dissipation dilution

    Tsaturyan, Yeghishe; Polzik, Eugene S; Schliesser, Albert


    The small mass and high coherence of nanomechanical resonators render them the ultimate force probe, with applications ranging from biosensing and magnetic resonance force microscopy, to quantum optomechanics. A notorious challenge in these experiments is thermomechanical noise related to dissipation through internal or external loss channels. Here, we introduce a novel approach to defining nanomechanical modes, which simultaneously provides strong spatial confinement, full isolation from the substrate, and dilution of the resonator material's intrinsic dissipation by five orders of magnitude. It is based on a phononic bandgap structure that localises the mode, without imposing the boundary conditions of a rigid clamp. The reduced curvature in the highly tensioned silicon nitride resonator enables mechanical $Q>10^{8}$ at $ 1 \\,\\mathrm{MHz}$, yielding the highest mechanical $Qf$-products ($>10^{14}\\,\\mathrm{Hz}$) yet reported at room temperature. The corresponding coherence times approach those of optically t...

  11. An integrated, low noise patch-clamp amplifier for biological nanopore applications.

    Wang, Gang; Dunbar, William B


    We present an integrated, low noise patch-clamp amplifier for biological nanopore applications. Our amplifier consists of an integrator-differentiator architecture coupled with a novel opamp design in the CMOS 0.35 µm process. The post-layout full-chip simulation shows the input referred noise of the amplifier is 0.49 pA RMS over a 5 kHz bandwidth using a verified electrical model for the biological nanopore system. In our biological nanopore experiments studying protein-DNA interactions, we encounter capacitive transients with a nominal settling time of 5 ms. Our amplifier design reduces the settling time to 0.2 ms, without requiring any compensation circuitry.

  12. Multi-mode excitation of a clamped–clamped microbeam resonator

    Younis, Mohammad I.


    We present modeling and simulation of the nonlinear dynamics of a microresonator subjected to two-source electrostatic excitation. The resonator is composed of a clamped–clamped beam excited by a DC voltage load superimposed to two AC voltage loads of different frequencies. One frequency is tuned close to the first natural frequency of the beam and the other is close to the third (second symmetric) natural frequency. A multi-mode Galerkin procedure is applied to extract a reduced-order model, which forms the basis of the numerical simulations. Time history response, Poincare’ sections, Fast Fourier Transforms FFT, and bifurcation diagrams are used to reveal the dynamics of the system. The results indicate complex nonlinear phenomena, which include quasiperiodic motion, torus bifurcations, and modulated chaotic attractors.

  13. Nonlinear dynamic behaviors of clamped laminated shallow shells with one-to-one internal resonance

    Abe, Akira; Kobayashi, Yukinori; Yamada, Gen


    This paper investigates one-to-one internal resonance of laminated shallow shells with rigidly clamped edges. It is assumed that the natural frequencies ω2 and ω3 of two asymmetric (second and third) vibration modes have the relationship ω2≈ ω3. The displacements are expressed by using eigenvectors for linear vibration modes calculated by the Ritz method. Applying Galerkin's procedure to the equation of motion, nonlinear differential equations are derived. By considering the first vibration mode in addition to the two asymmetric vibration modes, quadratic nonlinear terms expressing the interaction between the asymmetric and the first modes appear in the differential equations. Shooting method is used to obtain the steady-state response when the driving frequency Ω is near ω2. The dynamic characteristics of the shells with the internal resonance are discussed.

  14. A Color LED Driver Implemented by the Active Clamp Forward Converter

    C. H. Chang


    Full Text Available Because light emitting diodes (LEDs have the advantages of dc working voltage, high luminescent efficiency, shortignition time, high reliability and pollution free, they have substituted for incandescent bulbs and fluorescent lampsgradually. In order to simplify circuit complexity, an active clamp forward converter with the sequential color display(SCD control is proposed to drive red, green and blue (RGB LED arrays. The proposed converter has zero-voltageswitching (ZVS operations of both the main switch and the auxiliary switch, resulting in high system efficiency. DrivingRGB LED arrays sequentially by one converter can save components and reduce cost significantly. Additionally, thepulse-width modulation (PWM control is applied to achieve a large chromaticity variation. The circuit operations areanalyzed in detail and the circuit parameters are designed based on the practical considerations. Finally, an illustrativeexample is implemented to demonstrate the feasibility and validity of the proposed LED driver.

  15. Simultaneous multi-patch-clamp and extracellular-array recordings: Single neuron reflects network activity

    Vardi, Roni; Goldental, Amir; Sardi, Shira; Sheinin, Anton; Kanter, Ido


    The increasing number of recording electrodes enhances the capability of capturing the network’s cooperative activity, however, using too many monitors might alter the properties of the measured neural network and induce noise. Using a technique that merges simultaneous multi-patch-clamp and multi-electrode array recordings of neural networks in-vitro, we show that the membrane potential of a single neuron is a reliable and super-sensitive probe for monitoring such cooperative activities and their detailed rhythms. Specifically, the membrane potential and the spiking activity of a single neuron are either highly correlated or highly anti-correlated with the time-dependent macroscopic activity of the entire network. This surprising observation also sheds light on the cooperative origin of neuronal burst in cultured networks. Our findings present an alternative flexible approach to the technique based on a massive tiling of networks by large-scale arrays of electrodes to monitor their activity.

  16. A DTC Algorithm for Induction Motor Drives with 3-level Diode-Clamped Inverters

    M. Cirrincione


    Full Text Available This paper presents a novel Direct Torque Control (DTC algorithm for induction motor electrical drives supplied by 3-level diode clamped inverters. It is the natural extension of the classic Switching Table (ST based DTC, modified for a 3-level inverter supply. It presents also a very simple algorithm for the minimization of the capacitor voltage ripple. Results obtained with numerical simulations show that the employment of a 3-level inverter in such a control scheme permits to obtain the same dynamical performances as those obtained with a 2-level inverter with resulting lower torque and flux ripples as well as a lower harmonic content in the stator voltages and currents. Some considerations about the consequent benefits as for EMC of this drive are also presented.

  17. Macromechanical parametric amplification with a base-excited doubly clamped beam

    Neumeyer, Stefan; Thomsen, Jon Juel


    of steady-state vibration amplitude of the directly and parametrically excited system, to vibration amplitude of the directly excited only system. The nonlinear effect of midplane stretching is compared to the effects of nonlinear inertia and curvature. An approximate analytical expression for the vibration...... amplitude is derived. For a given small level of transverse displacement for both the cantilever and doubly clamped beam, the effect of midplane stretching is dominant compared to those caused by nonlinear inertia and curvature. It was found that the beam slenderness ratio can be used as an effective design......Parametric amplification is realized by adding parametric excitation to externally driven near-resonant oscillations. The effect of specific cubic nonlinearities on the parametrically amplified steady-state vibrations and gain is investigated theoretically. Here, gain is defined as the ratio...

  18. Multifrequency excitation of a clamped–clamped microbeam: Analytical and experimental investigation

    Jaber, Nizar


    Using partial electrodes and a multifrequency electrical source, we present a large-bandwidth, large-amplitude clamped–clamped microbeam resonator excited near the higher order modes of vibration. We analytically and experimentally investigate the nonlinear dynamics of the microbeam under a two-source harmonic excitation. The first-frequency source is swept around the first three modes of vibration, whereas the second source frequency remains fixed. New additive and subtractive resonances are demonstrated. We illustrated that by properly tuning the frequency and amplitude of the excitation force, the frequency bandwidth of the resonator is controlled. The microbeam is fabricated using polyimide as a structural layer coated with nickel from the top and chromium and gold layers from the bottom. Using the Galerkin method, a reduced order model is derived to simulate the static and dynamic response of the device. A good agreement between the theoretical and experimental data are reported.

  19. Comparative Analysis and Simulation of Diode Clamped & Cascaded H-Bridge Multilevel Inverter using SPWM Technique

    Mohit Jain


    Full Text Available Multilevel inverters have become more popular over the years in high power medium voltage applications without the use of a transformer and with promise of less disturbance & reduced harmonic distortion. In this paper, two types of multilevel converter in three phase configuration, cascaded H-Bridge multilevel inverter (CMLI and diode clamped multilevel inverter (DCMLI of 5 and 7-level are modelled and compared in the case of feeding of a three phase squirrel cage induction motor. Here, carrier based sinusoidal pulse width modulation (SPWM technique is used as the modulation strategy. These modulation strategy include phase disposition technique (PD, phase opposition disposition technique (POD, and an alternative phase opposition disposition technique (APOD. A detailed study of the modulation technique has been carried out through MATLAB/SIMULINK for both multilevel converters and a comparative evaluation between DCMLI and CMLI using SPWM technique in terms of THD%.




    Full Text Available This paper presents a simplified space vector pulse width modulation (SVPWM based diode clamped threelevel inverter fed direct torque controlled (DTC induction motor drive. The space vector diagram of three-level inverter is simplified into two-level inverter. So the selection of switching sequences is done as conventional two-level SVPWM method. Thus, the proposed algorithm reduces the complexity involved in the PWM algorithm. To validate the proposed PWM lgorithm, several simulation studies have been carried and resultsare presented. From the results, it can be observed that the proposed algorithm reduces the total harmonic distortion (THD of the line current and line voltages when compared with the 2-level inverter fed induction motor drive.

  1. Novel Full-bridge ZVS DC-DC Converter with an Clamp Diodes

    Guangqun, Nan; Xue, Hu

    The two arms of this converter all work at zero-voltage switching condition, that bring many advantages, such as little loss of power, simple control, etc. But these also exists some problems, such as parasitic oscillation on the output rectifier diodes. A novel full-bridge ZVS PWM DC-DC converter which adopts two clamping diodes in the first side of the transformer is proposed to reduce the parasitic oscillation in this paper. In this paper, It heavily analyzes the realization process of their soft-switching and the reason of related issues and solution, we establishes simulation model and simulates using orCAD. Finally,a 5 kW ZVS PWM DC-DC converter Prototype in that TMS320F2812 is the core controlled chip has been set up, and the experiments showed us the validation of the correlative theory.

  2. Exaggerated natriuresis during clamping of systemic NO supply in healthy young men

    Simonsen, Jane Angel; Rasmussen, Mona S; Vach, Werner


    NO (nitric oxide) may be involved in fluid homoeostasis. We hypothesized that increases in NO synthesis contribute to acute, saline-induced natriuresis, which, therefore, should be blunted when NO availability is stabilized. Young men were studied during simultaneous infusions of L-NAME [NG......-nitro-L-arginine methyl ester; bolus of 750 μg·kg-1 of body weight and 8.3 μg·min-1·kg-1 of body weight] and SNP (sodium nitroprusside), the latter at a rate preventing L-NAME from increasing total peripheral resistance ('NO-clamping'). Slow volume expansion (saline, 20 μmol of NaCl·min-1·kg-1 of body weight for 3 h...

  3. Screening system for drug-induced arrhythmogenic risk combining a patch clamp and heart simulator

    Okada, Jun-ichi; Yoshinaga, Takashi; Kurokawa, Junko; Washio, Takumi; Furukawa, Tetsushi; Sawada, Kohei; Sugiura, Seiryo; Hisada, Toshiaki


    To save time and cost for drug discovery, a paradigm shift in cardiotoxicity testing is required. We introduce a novel screening system for drug-induced arrhythmogenic risk that combines in vitro pharmacological assays and a multiscale heart simulator. For 12 drugs reported to have varying cardiotoxicity risks, dose-inhibition curves were determined for six ion channels using automated patch clamp systems. By manipulating the channel models implemented in a heart simulator consisting of more than 20 million myocyte models, we simulated a standard electrocardiogram (ECG) under various doses of drugs. When the drug concentrations were increased from therapeutic levels, each drug induced a concentration-dependent characteristic type of ventricular arrhythmia, whereas no arrhythmias were observed at any dose with drugs known to be safe. We have shown that our system combining in vitro and in silico technologies can predict drug-induced arrhythmogenic risk reliably and efficiently. PMID:26601174

  4. Visual patch clamp recording of neurons in thick portions of the adult spinal cord

    Munch, Anders Sonne; Smith, Morten; Moldovan, Mihai


    The study of visually identified neurons in slice preparations from the central nervous system offers considerable advantages over in vivo preparations including high mechanical stability in the absence of anaesthesia and full control of the extracellular medium. However, because of their relative...... thinness, slices are not appropriate for investigating how individual neurons integrate synaptic inputs generated by large numbers of neurons. Here we took advantage of the exceptional resistance of the turtle to anoxia to make slices of increasing thicknesses (from 300 to 3000 microm) from the lumbar...... enlargement of the spinal cord. With a conventional upright microscope in which the light condenser was carefully adjusted, we could visualize neurons present at the surface of the slice and record them with the whole-cell patch clamp technique. We show that neurons present in the middle of the preparation...

  5. Fabrication of Si-based planar type patch clamp biosensor using silicon on insulator substrate

    Zhang, Z.L.; Asano, T. [Graduate University for Advanced Studies, Myodaiji, Okazaki, 444-8585 (Japan); Uno, H. [Institute for Molecular Science, Myodaiji, Okazaki, 444-8585 (Japan); Tero, R. [Graduate University for Advanced Studies, Myodaiji, Okazaki, 444-8585 (Japan); Institute for Molecular Science, Myodaiji, Okazaki, 444-8585 (Japan); Suzui, M.; Nakao, S. [Institute for Molecular Science, Myodaiji, Okazaki, 444-8585 (Japan); Kaito, T. [SII NanoTechnology Inc., 36-1, Takenoshita, Oyama-cho, Sunto-gun, Shizuoka, 410-1393 (Japan); Shibasaki, K.; Tominaga, M. [Okazaki Institute for Integrative Bioscience, 5-1, Higashiyama, Myodaiji, Okazaki, 444-8787 (Japan); Utsumi, Y. [Laboratory of Advanced Science and Technology for Industry, University of Hyogo, 3-1-2, Koto, Kamigori, Ako-gun, Hyogo, 678-1205 (Japan); Gao, Y.L. [Department of Physics and Astronomy, Rochester University, Rochester, New York 14627 (United States); Urisu, T. [Graduate University for Advanced Studies, Myodaiji, Okazaki, 444-8585 (Japan); Institute for Molecular Science, Myodaiji, Okazaki, 444-8585 (Japan)], E-mail:


    The aim of this paper is to fabricate the planar type patch clamp ion-channel biosensor, which is suitable for the high throughput screening, using silicon-on-insulator (SOI) substrate. The micropore with 1.2 {mu}m diameter is formed through the top Si layer and the SiO{sub 2} box layer of the SOI substrate by focused ion beam (FIB). Then the substrate is assembled into the microfluidic circuit. The human embryonic kidney 293 (HEK-293) cell transfected with transient receptor potential vanilloid type 1 (TRPV1) is positioned on the micropore and the whole-cell configuration is formed by the suction. Capsaicin is added to the extracellular solution as a ligand molecule, and the channel current showing the desensitization unique to TRPV1 is measured successfully.

  6. Simple Switching Strategy for High-Torque Control Performance utilizing Neutral Point Clamped Multilevel Inverter

    Nor Faezah Alias


    Full Text Available Three-level Neutral Point Clamped (NPC inverter allows the configuration of switching devices to operate at high voltage and produce lower current/voltage harmonics. It is known that, DTC of induction machine which employs hysteresis controller has major drawbacks namely larger torque ripple and variable switching frequency. This paper aims to propose a suitable voltage vector selection to provide better torque regulation and lower switching frequency by employing DTC with 3-level NPC multilevel inverter. A simple switching strategy was formulated using 7-level torque hysteresis and 2-level flux hysteresis controllers to give more options in selecting an appropriate voltage vector, inherently, according the motor operation conditions. The improvements offered were verified through simulations.

  7. Comparative Evaluation of Pulsewidth Modulation Strategies for Z-Source Neutral-Point-Clamped Inverter

    Loh, P.C.; Blaabjerg, Frede; Wong, C.P.


    Z-source neutral-point-clamped (NPC) inverter has recently been proposed as an alternative three-level buck-boost power conversion solution with an improved output waveform quality. In principle, the designed Z-source inverter functions by selectively "shooting through" its power sources, coupled...... to the inverter using two unique Z-source impedance networks, to boost the inverter three-level output waveform. Proper modulation of the new inverter would therefore require careful integration of the selective shoot-through process to the basic switching concepts to achieve maximal voltage-boost, minimal...... that have been presented. The presented strategies are also comparatively evaluated to identify their individual advantages and disadvantages, which are subsequently summarized in a comprehensive reference table. Last, simulation and experimental results are included for validating the performances...

  8. Model Predictive Control of Z-source Neutral Point Clamped Inverter

    Mo, Wei; Loh, Poh Chiang; Blaabjerg, Frede


    This paper presents Model Predictive Control (MPC) of Z-source Neutral Point Clamped (NPC) inverter. For illustration, current control of Z-source NPC grid-connected inverter is analyzed and simulated. With MPC’s advantage of easily including system constraints, load current, impedance network...... inductor current, capacitor voltage as well as switching frequency, transient response are all regulated as subjecting to constraints of this control method. The quality of output waveform, stability of impedance-network, level constraint of variable switching frequency as well as robustness of transient...... response are obtained at the same time with a formulated Z-source NPC inverter network model. Operation steady state and transient state simulation results of MPC are going to be presented, which shows good reference tracking ability of this method. It provides new control method for Z-source NPC inverter...

  9. Note: Decoupling design for high frequency piezoelectric ultrasonic transducers with their clamping connections

    Wang, F. J., E-mail:; Liang, C. M.; Tian, Y. L.; Zhao, X. Y.; Zhang, D. W. [Tianjin Key Laboratory of Equipment Design and Manufacturing Technology, School of Mechanical Engineering, Tianjin University, Tianjin 300072 (China); Zhang, H. J. [Tianjin Key Laboratory of Modern Mechatronics Equipment Technology, School of Mechanical Engineering, Tianjin Polytechnic University, Tianjin 300387 (China)


    This work presents the flexure-mechanism based decoupling design between high frequency piezoelectric ultrasonic transducers and their clamping connections to improve ultrasonic energy transmission efficiency. The ring, prismatic beam, and circular notched hinge based flanges were presented, and the crucial geometric dimensions of the transducers with the flexure decoupling flanges were determined. Finite element analysis (FEA) was carried out to investigate the dynamic characteristics of the transducers. Finally, experiments were conducted to examine and verify the effects of the proposed decoupling flanges. FEA and experimental results show that smaller frequency deviations and larger tip displacement amplitudes have been achieved by using the transducers with the flexure flanges compared with the transducer with a rigid ring-type flange, and thus the ultrasonic transmission efficiency can be improved through the flexure flanges.

  10. A novel latch-up free SCR-LDMOS with high holding voltage for a power-rail ESD clamp

    Pan Hongwei; Liu Siyang; Sun Weifeng


    The low snapback holding voltage of the SCR-LDMOS device makes it susceptible to latch-up failure,when used in power-rail ESD (electro-static discharge) clamp circuits.In order to eliminate latch-up risk,this work presents a novel SCR-LDMOS structure with an N-type implantation layer to achieve a 17 V holding voltage and a 5.2 A second breakdown current.The device has been validated using TLP measurement analysis and is applied to a power-rail ESD clamp in half-bridge driver ICs.

  11. MATLAB-based automated patch-clamp system for awake behaving mice.

    Desai, Niraj S; Siegel, Jennifer J; Taylor, William; Chitwood, Raymond A; Johnston, Daniel


    Automation has been an important part of biomedical research for decades, and the use of automated and robotic systems is now standard for such tasks as DNA sequencing, microfluidics, and high-throughput screening. Recently, Kodandaramaiah and colleagues (Nat Methods 9: 585-587, 2012) demonstrated, using anesthetized animals, the feasibility of automating blind patch-clamp recordings in vivo. Blind patch is a good target for automation because it is a complex yet highly stereotyped process that revolves around analysis of a single signal (electrode impedance) and movement along a single axis. Here, we introduce an automated system for blind patch-clamp recordings from awake, head-fixed mice running on a wheel. In its design, we were guided by 3 requirements: easy-to-use and easy-to-modify software; seamless integration of behavioral equipment; and efficient use of time. The resulting system employs equipment that is standard for patch recording rigs, moderately priced, or simple to make. It is written entirely in MATLAB, a programming environment that has an enormous user base in the neuroscience community and many available resources for analysis and instrument control. Using this system, we obtained 19 whole cell patch recordings from neurons in the prefrontal cortex of awake mice, aged 8-9 wk. Successful recordings had series resistances that averaged 52 ± 4 MΩ and required 5.7 ± 0.6 attempts to obtain. These numbers are comparable with those of experienced electrophysiologists working manually, and this system, written in a simple and familiar language, will be useful to many cellular electrophysiologists who wish to study awake behaving mice.

  12. Characterization of ryanodine receptor type 1 single channel activity using "on-nucleus" patch clamp.

    Wagner, Larry E; Groom, Linda A; Dirksen, Robert T; Yule, David I


    In this study, we provide the first description of the biophysical and pharmacological properties of ryanodine receptor type 1 (RyR1) expressed in a native membrane using the on-nucleus configuration of the patch clamp technique. A stable cell line expressing rabbit RyR1 was established (HEK-RyR1) using the FLP-in 293 cell system. In contrast to untransfected cells, RyR1 expression was readily demonstrated by immunoblotting and immunocytochemistry in HEK-RyR1 cells. In addition, the RyR1 agonists 4-CMC and caffeine activated Ca(2+) release that was inhibited by high concentrations of ryanodine. On nucleus patch clamp was performed in nuclei prepared from HEK-RyR1 cells. Raising the [Ca(2+)] in the patch pipette resulted in the appearance of a large conductance cation channel with well resolved kinetics and the absence of prominent subconductance states. Current versus voltage relationships were ohmic and revealed a chord conductance of ∼750pS or 450pS in symmetrical 250mM KCl or CsCl, respectively. The channel activity was markedly enhanced by caffeine and exposure to ryanodine resulted in the appearance of a subconductance state with a conductance ∼40% of the full channel opening with a Po near unity. In total, these properties are entirely consistent with RyR1 channel activity. Exposure of RyR1 channels to cyclic ADP ribose (cADPr), nicotinic acid adenine dinucleotide phosphate (NAADP) or dantrolene did not alter the single channel activity stimulated by Ca(2+), and thus, it is unlikely these molecules directly modulate RyR1 channel activity. In summary, we describe an experimental platform to monitor the single channel properties of RyR channels. We envision that this system will be influential in characterizing disease-associated RyR mutations and the molecular determinants of RyR channel modulation.

  13. Evaluating LMA and CLAMP: Using information criteria to choose a model for estimating elevation

    Miller, I.; Green, W.; Zaitchik, B.; Brandon, M.; Hickey, L.


    The morphology of leaves and composition of the flora respond strongly to the moisture and temperature of their environment. Elevation and latitude correlate, at first order, to these atmospheric parameters. An obvious modern example of this relationship between leaf morphology and environment is the tree line, where boreal forests give way to artic (high latitude) or alpine (high elevation) tundra. Several quantitative methods, all of which rely on uniformitarianism, have been developed to estimate paleoelevation using fossil leaf morphology. These include 1) the univariate leaf-margin analysis (LMA), which estimates mean annual temperature (MAT) by the positive linear correlation between MAT and P, the proportion of entire or smooth to non-entire or toothed margined woody dicot angiosperm leaves within a flora and 2) the Climate Leaf Analysis Multivariate Program (CLAMP) which uses Canonical Correspondence Analysis (CCA) to estimate MAT, moist enthalpy, and other atmospheric parameters using 31 explanatory leaf characters from woody dicot angiosperms. Given a difference in leaf-estimated MAT or moist enthalpy between contemporaneous, synlatitudinal fossil floras-one at sea-level, the other at an unknown paleoelevation-paleoelevation may be estimated. These methods have been widely applied to orogenic settings and concentrate particularly in the Western US. We introduce the use of information criteria to compare different models for estimating elevation and show how the additional complexity of the CLAMP analytical methodology does not necessarily improve on the elevation estimates produced by simpler regression models. In addition, we discuss the signal-to-noise ratio in the data, give confidence intervals for detecting elevations, and address the problem of spatial autocorrelation and irregular sampling in the data.

  14. A clamp fixture with interdigital capacitive sensor for in situ evaluation of wire insulation

    Sheldon, Robert T.; Bowler, Nicola


    An interdigital capacitive sensor has been designed and optimized for testing aircraft wires by applying a quasinumerical model developed and reported previously. The sensor consists of two patches of interdigitated electrodes, connected by a long signal bus strip, that are intended to conform to two sides of an insulated wire. The electrodes are deposited using photolithography upon a 25.4-μm-thick Kapton® polyimide film. The two electrode patches are attached to the two jaws of a plastic spring-loaded clamp, with each jaw having a milled groove designed such that the electrodes conform to the curved surface of the insulated wire. An SMA connector and cable connect between the electrodes on the clamp and an LCR meter. Segments of pristine M5086/2 aircraft wire, each 10 cm long, were immersed in fluids commonly found in aircraft environments, to cause accelerated chemical degradation. The effects of Jet A fuel, deicing fluid, hydraulic fluid, aircraft cleaner, isopropyl alcohol and distilled water were studied. The frequency-dependent capacitance and dissipation factor of one pristine wire segment and of those degraded in the six fluid environments were measured within the frequency range 100 Hz to 1 MHz. Significant changes in capacitance and dissipation factor were observed for all degraded wires, compared with results for the pristine sample, suggesting the feasibility of detecting insulation degradation in the field. The results were also consistent with those of a similar experiment performed on sheets of Nylon 6, the material that comprises the outermost layer of M5086/2 wire.

  15. Comparative Analysis of PWM Techniques for Three Level Diode Clamped Voltage Source Inverter

    Zulkifilie Bin Ibrahim


    Full Text Available Multilevel inverters are increasingly being used in high-power medium voltage industrial drive applications due to their superior performance compared to conventional two-level inverters. Thre are a number of Pulse width modulation (PWM techniques applied in recent years. The most widely applied PWM techniques are Sine Pulse Width Modulation (SPWM and Space Vector Pulse Width Modulation (SVPWM.  SPWM is the most simple modulation technique that can realize easily in analog circuit.  However, it has some drawbacks such as higher total harmonic distortion (THD, lower effective DC utilization and lower switching frequency. Space vector pulse width modulation (SVPWM is widely used because of their easier digital realization and better DC bus utilization and lower THD. The complexity is due to the difficulty in determining the reference vector location, on times calculation, and switching states selection. This paper presents a simple SVPWM algorithm for diode clamped three level inverters based on standard two-level SVPWM which can easily determine the location of reference vector, calculate the on-times, the selection of switching states. Three level diode clamped inverter (3LDCI using space vector modulation technique has been modeled and simulated using MATLAB/SIMULINK and Origin 6.1 with a passive R-L load that can be extended to any level. Simulation results are presented to verify the proposed SVPWM control in terms of THD. The results are compared with conventional sinusoidal pulse width modulation (SPWM where SVPWM shows better performance than SPWM in terms of THD.

  16. Management of open olecranon fractures using clamp-cum-compressor device

    Kundu Zile


    Full Text Available Background: Open fractures of olecranon are not a rare occurrence in patients with road traffic accidents particularly motor bike riders who don′t use elbow guards. Definitive treatment has to be delayed in many till the wound heals. The present study was conducted to evaluate the results of open fractures of olecranon using clamp-cum-compressor device. Materials and Methods: Seventeen patients between the ages of 20 and 45 years of open olecranon fractures reported 5-20 days after injury were treated using an indigenous clamp-cum-compressor. All fractures were Mayo type II-A, i.e., displaced, stable and noncomminuted. Four patients had Gustilo-Anderson grade I and 13 had Gustilo-Anderson grade II open fractures. The patients with transverse or short oblique fractures were included in the study. The apparatus was applied under regional anesthesia after thorough washing and debridement of wounds with few loose sutures applied wherever needed. The wounds healed within 2-4 weeks and fractures united within 8-10 weeks. The elbow was mobilized with apparatus still in place. The results were evaluated by MayoElbow performance score. Results: We achieved excellent results in twelve patients, good in four and poor in one patient, who reported late, hooks of the apparatus were cut through the proximal fragment, leading to union of fracture in elongation and restricted elbow movements. Conclusion: The apparatus was found to be quite useful in transverse and short oblique fractures with contamination or infection, where internal fixation has to be delayed or avoided.

  17. Tne stiffness of first-order and second-order modules assembled with extracortical clamp devices

    F. K. Sabirov


    Full Text Available The extracortical clamp device (ECD is a tool used in external fixation which unlike the K-wires and half-pins don’t perforate cortical bone. The use of ECD is prospective for the treatment of periprosthetic femoral fractures and in the lengthening over nail and bone transport over nail. The data on the bench tests of the osteosynthesis rigidity by the external fixation first-order and second-order modules on the base of extracortical clamp devices are observed in the article. Materials and methods. The authors made 240 bench tests using torsional-vibration machine, Indicators measuring linear displacements with a scale of 0.01 mm, bone simulators («Sawbones», calibrated load, Ilizarov’s apparatus и extracortical fixators. The statistical analysis was performed with use software «STATISTICA» (ver. 6.0. The data obtained are presented in graphs «Box and Whisker Plot». Results. Among the investigated variants of first-order modules the better results of osteosynthesis rigidity were found in the module based on two ECD inserted at angle 60 degrees to each other and at distance of 10 cm from each other. Among the investigated second-order modules, better results of osteosynthesis rigidity were found in the module based on two ECD inserted at an angle 60 degrees to each other at distance of 10 cm from each other. Conclusion. Thus the tested modules can be used in practice in assemblies of external fixation devices in periprosthetic fractures, lengthening and bone transport over the nail.

  18. 18 CFR 3c.1 - Cross-reference to employee ethical conduct standards and financial disclosure regulations.


    ... employee ethical conduct standards and financial disclosure regulations. 3c.1 Section 3c.1 Conservation of... STANDARDS OF CONDUCT § 3c.1 Cross-reference to employee ethical conduct standards and financial disclosure... branch-wide financial disclosure regulations at 5 CFR part 2634, the Standards of Ethical Conduct...

  19. File list: Oth.Adp.05.Nr3c1.AllCell [Chip-atlas[Archive

    Full Text Available Oth.Adp.05.Nr3c1.AllCell mm9 TFs and others Nr3c1 Adipocyte SRX821805,SRX821802,SRX...821803,SRX821804 ...

  20. Optical conductivity in A3C60 (A=K, Rb)

    Brink, J. van den; Gunnarsson, O.; Eyert, V.


    Published in: Phys. Rev. B 57 (1998) 2163-2167 Citing articles (CrossRef) citations recorded in [Science Citation Index] Abstract: We study the optical conductivity in A3C60 (A =K, Rb). The effects of the electron-phonon interaction is included to lowest order in the coupling strength lambda. It is

  1. VLBI, MERLIN and HST observations of the giant radio galaxy 3C 236

    Schilizzi, RT; Tian, WW; Conway, JE; Nan, R; Miley, GK; Barthel, PD; Normandeau, M; Dallacasa, D; Gurvits, LI


    We present VLBI and MERLIN data at 1.66 and 4.99 GHz on the central component coincident with the nucleus of the giant radio galaxy, 3C 236. The nuclear radio structure is composed of two complexes of emission which are resolved on scales from 1 milli-arcsec (mas) to 1 arcsec. Oscillations with an a

  2. Structural basis for molecular discrimination by a 3',3'-cGAMP sensing riboswitch.

    Ren, Aiming; Wang, Xin C; Kellenberger, Colleen A; Rajashankar, Kanagalaghatta R; Jones, Roger A; Hammond, Ming C; Patel, Dinshaw J


    Cyclic dinucleotides are second messengers that target the adaptor STING and stimulate the innate immune response in mammals. Besides protein receptors, there are bacterial riboswitches that selectively recognize cyclic dinucleotides. We recently discovered a natural riboswitch that targets 3',3'-cGAMP, which is distinguished from the endogenous mammalian signal 2',3'-cGAMP by its backbone connectivity. Here, we report on structures of the aptamer domain of the 3',3'-cGAMP riboswitch from Geobacter in the 3',3'-cGAMP and c-di-GMP bound states. The riboswitch adopts a tuning fork-like architecture with a junctional ligand-binding pocket and different orientations of the arms are correlated with the identity of the bound cyclic dinucleotide. Subsequent biochemical experiments revealed that specificity of ligand recognition can be affected by point mutations outside of the binding pocket, which has implications for both the assignment and reengineering of riboswitches in this structural class.

  3. Semipolar (202̅3) nitrides grown on 3C-SiC/(001) Si substrates

    Dinh, Duc V.; Presa, S.; Akhter, M.; Maaskant, P. P.; Corbett, B.; Parbrook, P. J.


    Heteroepitaxial growth of GaN buffer layers on 3C-SiC/(001) Si templates (4°-offcut towards [110]) by metalorganic vapour phase epitaxy has been investigated. High-temperature grown Al0.5Ga0.5N/AlN interlayers were employed to produce a single (202̅3) GaN surface orientation. Specular crack-free GaN layers showed undulations along [11̅0]{}3{{C}-{SiC}/{Si}} with a root mean square roughness of about 13.5 nm (50 × 50 μm2). The orientation relationship determined by x-ray diffraction (XRD) was found to be [1̅21̅0]GaN ∥[11̅0]{}3{{C}-{SiC}/{Si}} and [3̅034]GaN ∥[110]3C - SiC/Si . Low-temperature photoluminescence (PL) and XRD measurements showed the presence of basal-plane stacking faults in the layers. PL measurements of (202̅3) multiple-quantum-well and light-emitting diode structures showed uniform luminescence at about 500 nm emission wavelength. A small peak shift of about 3 nm was observed in the electroluminescence when the current was increased from 5 to 50 mA (25-250 A cm-2).

  4. Human rhinovirus 3C protease as a potential target for the development of antiviral agents.

    Wanga, Q May; Chen, Shu-Hui


    As the major cause of the common cold in children and adults, human rhinoviruses (HRVs) are a group of small single-stranded positive-sense RNA viruses. HRVs translate their genetic information into a polyprotein precursor that is mainly processed by a virally encoded 3C protease (3Cpro) to generate functional viral proteins and enzymes. It has been shown that the enzymatic activity of HRV 3Cpro is essential to viral replication. The 3Cpro is distinguished from most other proteases by the fact that it has a cysteine nucleophile but with a chymotrypsin-like serine protease folding. This unique protein structure together with its essential role in viral replication made the 3Cpro an excellent target for antiviral intervention. In recent years, considerable efforts have been made in the development of antiviral compounds targeting this enzyme. To further facilitate the design of potent 3C protease inhibitors for therapeutic use, this review summarizes the biochemical and structural characterization conducted on HRV 3C protease along with the recent progress on the development of 3C protease inhibitors.

  5. Studying physical chromatin interactions in plants using Chromosome Conformation Capture (3C)

    Louwers, M.; Splinter, E.; van Driel, R.; de Laat, W.; Stam, M.


    Gene regulation in higher eukaryotes frequently involves physical interactions between genomic sequence elements tens of kilobases apart on the same chromosome but can also entail interactions between different chromosomes. Chromosome Conformation Capture (3C) is a powerful tool to identify such int

  6. Studying physical chromatin interactions in plants using Chromosome Conformation Capture (3C).

    Louwers, M.; Splinter, E.; van Driel, R.; de Laat, W.; Stam, M.


    Gene regulation in higher eukaryotes frequently involves physical interactions between genomic sequence elements tens of kilobases apart on the same chromosome but can also entail interactions between different chromosomes. Chromosome Conformation Capture (3C) is a powerful tool to identify such int

  7. Superluminal motion in a compact steep spectrum radio source 3C 138

    Shen, Z Q; Kameno, S; Chen, Y J


    We present the results of 5 GHz VLBI observations of a compact steep spectrum source 3C 138. The data are consistent with the western end being the location of the central activity. The observed offset between different frequencies in the central region of 3C 138 can be accounted for by a frequency dependent shift of the synchrotron self-absorbed core. Our new measurements confirm the existence of a superluminal motion, but its apparent velocity of 3.3c is three times slower than the reported one. This value is consistent with the absence of parsec-scale counter-jet emission in the inner region, but seems still too high to allow the overall counter-jet to be seen in terms of Doppler boosting of an intrinsically identical jet. Either an interaction of jet with central dense medium, or an intrinsically asymmetrical jet must be invoked to reconcile the detected superluminal speed with the observed large scale asymmetry in 3C 138.

  8. Kinematics of the Parsec-Scale Relativistic Jet in Quasar 3C 279 1991 - 1997

    Wehrle, A E; Unwin, S C; Zook, A C; Xu, W; Marscher, A P; Terasranta, H; Valtaoja, E


    We present results of long-term high-frequency VLBI monitoring of the relativistic jet in 3C279, consisting of 18 epochs at 22 GHz from 1991 to 1997 and 10 epochs at 43 GHz from 1995 to 1997. Three major results of this study are: apparent speeds measured for six superluminal components range from 4.8 to 7.5 c (H_{0}=70 km s^{-1} Mpc^{-1}, q_{0}=0.1), variations in the total radio flux are due primarily to changes in the VLBI core flux, and the uniform-sphere brightness temperature of the VLBI core is about 1 x 10^{13} K at 22 GHz after 1995, one of the highest direct estimates of a brightness temperature. If the variability brightness temperature measured for 3C279 by Lahteenmaki & Valtaoja is an actual value and not a lower limit, then the rest-frame brightness temperature of 3C279 is quite high and limited by inverse Compton effects rather than equipartition. The parsec-scale morphology of 3C279 consists of a bright, compact VLBI core, a jet component (C4) that moved from about 2 mas to about 3.5 mas f...

  9. The nature of the jet-driven outflow in the radio galaxy 3C 305

    Hardcastle, M. J.; Massaro, F.; Harris, D. E.; Baum, S. A.; Bianchi, S.; Chiaberge, M.; Morganti, R.; O'Dea, C. P.; Siemiginowska, A.


    We present Chandra X-ray and Very Large Array radio observations of the radio galaxy 3C?305. The X-ray observations reveal the details of the previously known extended X-ray halo around the radio galaxy. We show using X-ray spectroscopy that the X-ray emission is consistent with being shock-heated m

  10. Fast outflow of neutral and ionized gas from the radio galaxy 3C 293

    Emonts, B.H.C.; Morganti, R.; Oosterloo, T.A.; Tadhunter, C.N.; Hulst, J.M. van der


    Abstract: We detect a fast outflow of neutral and ionized gas with velocities up to about 1000 km/s from the central region of radio galaxy 3C 293. With optical spectroscopy we locate the bulk of the ionized gas outflow at the position of a bright radio hot-spot in the inner radio jet, about 1 kpc e

  11. Fast outflow of neutral and ionized gas from the radio galaxy 3C 293

    Emonts, BHC; Morganti, R; Osterloo, TA; Tadhunter, CN; van der Hulst, JM; Braun, R


    We detect a fast outflow of neutral and ionized gas with velocities up to similar to 1000 km/s from the central region of radio galaxy 3C 293. With optical spectroscopy we locate the bulk of the ionized gas outflow at the position of a bright radio hot-spot in the inner radio jet, about I kpc east o

  12. The nature of the jet-driven outflow in the radio galaxy 3C 305

    Hardcastle, M. J.; Massaro, F.; Harris, D. E.; Baum, S. A.; Bianchi, S.; Chiaberge, M.; Morganti, R.; O'Dea, C. P.; Siemiginowska, A.


    We present Chandra X-ray and Very Large Array radio observations of the radio galaxy 3C 305. The X-ray observations reveal the details of the previously known extended X-ray halo around the radio galaxy. We show using X-ray spectroscopy that the X-ray emission is consistent with being shock-heated m

  13. Identification of gamma-ray emission from 3C345 and NRAO512

    Schinzel, F K; D'Ammando, F; Burnett, T H; Max-Moerbeck, W; Cheung, C C; Fegan, S J; Casandjian, J M; Reyes, L C; Villata, M; Raiteri, C M; Agudo, I; Calle, O J A Bravo; Carosati, D; Casas, R; Gomez, J L; Gurwell, M A; Hsiao, H Y; Jorstad, S G; Kimeridze, G; Konstantinova, T S; Kopatskaya, E N; Koptelova, E; Kurtanidze, O M; Kurtanidze, S O; Larionov, V M; Larionova, E G; Larionova, L V; Marscher, A P; Morozova, D A; Nikolashvili, M G; Roca-Sogorb, M; Ros, J A; Sigua, L A; Spiridonova, O; Troitsky, I S; Vlasyuk, V V; Lobanov, A P; Zensus, J A


    For more than 15 years, since the days of the Energetic Gamma-Ray Experiment Telescope (EGRET) on board the Compton Gamma-Ray Observatory (CGRO; 1991-2000), it has remained an open question why the prominent blazar 3C 345 was not reliably detected at gamma-ray energies <=20 MeV. Recently a bright gamma-ray source (0FGL J1641.4+3939/1FGL J1642.5+3947), potentially associated with 3C 345, was detected by the Large Area Telescope (LAT) on Fermi. Multiwavelength observations from radio bands to X-rays (mainly GASP-WEBT and Swift) of possible counterparts (3C 345, NRAO 512, B3 1640+396) were combined with 20 months of Fermi-LAT monitoring data (August 2008 - April 2010) to associate and identify the dominating gamma-ray emitting counterpart of 1FGL J1642.5+3947. The source 3C 345 is identified as the main contributor for this gamma-ray emitting region. However, after November 2009 (15 months), a significant excess of photons from the nearby quasar NRAO 512 started to contribute and thereafter was detected with ...

  14. 3C 273 with NuSTAR: Unveiling the Active Galactic Nucleus

    Madsen, Kristin K.; Fürst, Felix; Walton, Dominic J.


    We present results from a 244 ks NuSTAR observation of 3C 273 obtained during a cross-calibration campaign with the Chandra, INTEGRAL, Suzaku, Swift, and XMM-Newton observatories. We show that the spectrum, when fit with a power-law model using data from all observatories except INTEGRAL over the...

  15. X-ray Emission from the Radio Jet in 3C 120

    Harris, D. E.; Hjorth, J.; Sadun, A. C.;


    We report the discovery of X-ray emission from a radio knot at a projected distance of 25" from the nucleus of the Seyfert galaxy, 3C 120. The data were obtained with the ROSAT High Resolution Imager (HRI). Optical upper limits for the knot preclude a simple power law extension of the radio spect...

  16. Optical Monitoring of 3C 66A in 1994–2008

    J. Tao; J. H. Fan; B. C. Qian; H. J. Pan


    3C 66A is one of the most interesting blazar. Our monitoring was carried out with a 1.56-m telescope of the Shanghai Astronomical Observatory (SHAO) from 13 December 1994 to 9 November 2008. Some peaks and gradual brightening of the source up to three times were observed.

  17. LM-3C:Breakthrough in Launch Technology for Deep Space Exploration

    Li Dan; Hu Wei; Wei Yuanming


    @@ One of the main objectives ofthe Chang'e 2 mission was to demonstrate the direct Earthmoon transfer orbit launch technology.On October 1, 2010, a LM-3C launch vehicle sent the Chang'e 2 into its preset orbit accurately, demonstrating that China has made a breakthrough in launch technology for deep space exploration.

  18. LM-3B/E Launched Eutelsat's W3C Satellite

    Bian Ji


    At 16:21 Beijing time on October 7,2011,China successfully launched the W3C commercial telecommunications satellite manufactured by Thales Alenia Space for Eutelsat Communications into geostationary transfer orbit by using an enhanced LM-3B (LM-3B/E) launch vehicle from Xichang Satellite Launch Center.

  19. Thermally stimulated conductivity and thermoluminescence from Al2O3 : C

    Agersnap Larsen, N.; Bøtter-Jensen, L.; McKeever, S.W.S.


    Simultaneous measurements of thermoluminescence (TL) and thermally stimulated conductivity (TSC) are reported on single-crystal dosimetry-quality Al2O3:C following gamma irradiation at room temperature. Analysis of the data reveals a superposition of several first-order TL and TSC peaks caused...

  20. The corona of the broad-line radio galaxy 3C 390.3

    Lohfink, A. M.; Ogle, P.; Tombesi, F.;


    -UVOT data, we find indications that the compactness of the corona allows only a small fraction of the total UV/optical flux to be Comptonized. Our analysis of the reprocessed emission show that 3C 390.3 only has a small amount of reflection (R ~ 0.3), and of that the vast majority is from distant neutral...

  1. [μ-Bis(diphenylarsinomethane-1:2κ2As:As′]nonacarbonyl-1κ3C,2κ3C,3κ3C-[tris(4-fluorophenylphosphine-3κP]-triangulo-triruthenium(0

    Hoong-Kun Fun


    Full Text Available In the title triangulo-triruthenium compound, [Ru3(C25H22(As2(C18H12F3P(CO9], the bis(diphenylarsinomethane ligand bridges an Ru—Ru bond and the monodentate phosphine ligand bonds to the third Ru atom. Both the phosphine and arsine ligands are equatorial with respect to the Ru3 triangle. Additionally, each Ru atom carries one equatorial and two axial terminal carbonyl ligands. The three phosphine-substituted rings make dihedral angles of 87.76 (13, 57.43 (13 and 73.81 (12° with each other. The dihedral angles between the pairs of rings are 69.78 (14 and 83.38 (16° for the two diphenylarsino groups. In the crystal packing, molecules are linked by intermolecular C—H...F and C—H...O hydrogen bonds, forming two-dimensional planes parallel to the ab plane. These planes are also linked by intermolecular C—H...O hydrogen bonds into a three-dimensional framework. Intermolecular C—H...π interactions further stabilize the crystal structure.

  2. (Benzyldiphenylphosphine-3κP[μ-bis(diphenylarsinomethane-1:2κ2As:As′]nonacarbonyl-1κ3C,2κ3C,3κ3C-triangulo-triruthenium(0

    Omar bin Shawkataly


    Full Text Available The asymmetric unit of the title triangulo-triruthenium compound, [Ru3(C25H22As2(C19H17P(CO9], consists of two crystallographically independent molecules of the triangulo-triruthenium complex, A and B. The bis(diphenylarsinomethane ligand bridges an Ru—Ru bond and the monodentate phosphine ligand bonds to the third Ru atom. Both the phosphine and arsine ligands are equatorial with respect to the Ru3 triangle. In addition, each Ru atom carries one equatorial and two axial terminal carbonyl ligands. With regard to the three phosphine-substituted rings, the benzyl ring makes dihedral angles of 41.0 (3 and 43.9 (3° with the other two benzene rings in molecule A; these angles are 49.8 (3 and 56.8 (3° in molecule B. The dihedral angles between the two benzene rings are 76.1 (3 and 88.2 (3° for the two diphenylarsino groups in molecule A and 71.3 (3 and 78.1 (3° in molecule B. In the crystal packing, molecules are linked into chains via intermolecular C—H...O hydrogen bonds. Weak intermolecular C—H...π interactions further stabilize the crystal structure.

  3. [μ-Bis(diphenylphosphanylethane-1:2κ2P:P′]nonacarbonyl-1κ3C,2κ3C,3κ3C-(triphenylstibine-3κSb-triangulo-triruthenium(0

    Omar bin Shawkataly


    Full Text Available The asymmetric unit of the title triangulo-triruthenium compound, [Ru3(C26H24P2(C18H15Sb(CO9], consists of two crystallographically independent molecules, A and B. The bis(diphenylphosphanylethane ligand bridges an Ru—Ru bond and the monodentate stibine ligand bonds to the third Ru atom. Both the stibine and phosphine ligands are equatorial with respect to the Ru3 triangle. Additionally, each Ru atom carries one equatorial and two axial terminal carbonyl ligands. The three stibine-substituted benzene rings make dihedral angles of 38.7 (3, 71.5 (3 and 70.0 (3° with each other in molecule A whereas these angles are 83.9 (3, 88.2 (3 and 56.8 (3° in molecule B. Similarly, the dihedral angles between the two benzene rings are 80.7 (3 and 87.6 (3° for the two diphenylphosphanyl groups in molecule A and 84.0 (3 and 72.6 (4° in molecule B. In the crystal, molecules are linked into tetramers via intermolecular C—H...O hydrogen bonds. Weak intermolecular C—H...π interactions further stabilize the crystal structure.

  4. [μ-Bis(diphenylarsinomethane-1:2κ2As:As′]nonacarbonyl-1κ3C,2κ3C,3κ3C-[(pentafluorophenyldiphenylphosphine-3κP]-triangulo-triruthenium(0 chloroform monosolvate

    Omar bin Shawkataly


    Full Text Available The asymmetric unit of the title triangulo-triruthenium compound, [Ru3(C25H22As2(C18H10F5P(CO9]·CHCl3, contains one molecule of the triangulo-triruthenium complex and one molecule of the disordered chloroform solvent. The bis(diphenylarsinomethane ligand bridges an Ru—Ru bond and the monodentate phosphine ligand bonds to the third Ru atom. Both the arsine and phosphine ligands are equatorial with respect to the Ru3 triangle. In addition, each Ru atom carries one equatorial and two axial terminal carbonyl ligands. The phosphine-substituted benzene rings make dihedral angles of 68.43 (15, 65.14 (14 and 89.75 (14° with each other. The dihedral angles between the two benzene rings are 80.70 (15 and 84.53 (16° for the two diphenylarsino groups. In the crystal packing, the molecules are linked into a plane parallel to bc by intermolecular C—H...O and C—H...F hydrogen bonds. Weak intermolecular C—H...π interactions further stabilize the crystal structure.

  5. [μ-Bis(diphenylarsinomethane-1:2κ2As:As′]nonacarbonyl-1κ3C,2κ3C,3κ3C-[tris(4-methylphenylphosphine-3κP]-triangulo-triruthenium(0

    Omar bin Shawkataly


    Full Text Available In the title triangulo-triruthenium compound, [Ru3(C25H22As2(C21H21P(CO9], the bis(diphenylarsinomethane ligand bridges a Ru—Ru bond and the monodentate phosphine ligand bonds to the third Ru atom. Both the phosphine and arsine ligands are equatorial with respect to the Ru3 triangle. Additionally, each Ru atom carries one equatorial and two axial terminal carbonyl ligands. The three phenyl rings of the phosphine make dihedral angles of 86.89 (19, 82.1 (2 and 63.0 (2° with each other. The dihedral angles between the two phenyl rings are 73.8 (2 and 82.2 (3° for the two diphenylarsino groups. An intramolecular C—H...O hydrogen bond stabilizes the molecular structure. In the crystal packing, molecules are linked into chains down the b axis via intermolecular C—H...O hydrogen bonds.

  6. Design and Transplantation of Bootloader Based on S3C2440%基于S3C2440的Bootloader的分析与设计

    符秋丽; 黄金龙


    通过修改U-boot-1.1.6版本的源码,设计并实现了从NAND Flash和NOR Flash两种启动的嵌入式Bootloader,并将其移植到S3C2440微处理器的嵌入式系统上.对bootloader的设计决定了实现的bootloader不仅仅起到加载内核镜像这一基本功能,而是把bootloader看作是一个虚拟的小系统,让其对硬件板级系统有更多的支持以为系统开发者提供方便.

  7. Improvement of Reversing Valve of Downhole Boosting Device Used for Expansion Tube%膨胀管用井下增压装置换向阀改进



    在进行膨胀管加固作业时,因为井下增压装置的液压换向阀失效,导致膨胀头卡死,膨胀管未完全膨胀,使施工失败。分析原因是换向阀阀杆磨损造成的。对阀杆进行陶瓷喷涂处理,提高其耐磨性能。将压力换向改为增压活塞运动位置控制换向。改进后的换向阀提高了使用寿命,为井下增压膨胀管加固技术的施工安全提供保障。%In the operation of the expansion tube reinforcement,because of the failure of the revers-ing valve of the downhole boosting device,the expansion head is stuck,the expansion tube is not fully expanded,so that the construction failure.Analysis is caused by the valve stem wear.Ceram-ic coating was done on the valve stem to improve its wear resistance and change the pressure di-rection to the piston movement position control of the piston.The improved reversing valve has improved the working life,and has provided support for the construction safety of the downhole booster expansion tube reinforcement technology.

  8. Characterization of heterologously expressed transporter genes by patch- and voltage-clamp methods: Application to cyclic nucleotide-dependent responses

    Lemtiri-Chlieh, Fouad


    The application of patch- and voltage-clamp methods to study ion transport can be limited by many hurdles: the size of the cells to be patched and/or stabbed, the subcellular localization of the molecule of interest, and its density of expression that could be too low even in their own native environment. Functional expression of genes using recombinant DNA technology not only overcomes those hurdles but also affords additional and elegant investigations such as single-point mutation studies and subunit associations/regulations. In this chapter, we give a step-by-step description of two electrophysiological methods, patch clamp and two-electrode voltage clamp (TEVC), that are routinely used in combination with heterologous gene expression to assist researchers interested in the identification and characterization of ion transporters. We describe how to (1) obtain and maintain the cells suitable for the use with each of the above-mentioned methods (i.e., HEK-293 cells and yeast spheroplasts to use with the patch-clamp methodology and Xenopus laevis oocytes with TEVC), (2) transfect/inject them with the gene of interest, and (3) record ion transport activities. © Springer Science+Business Media New York 2013.

  9. Measurement of the cell membrane capacitance and conductance of colonic crypt cells of the rat using the patch clamp technique

    Schill, C


    Using the patch clamp technique the membrane capacitance and membrane conductance of colonic crypt cells of the rat was measured. The influence of the intracellular agonists Ca++, cAMP and of osmotic changes on the membrane capacitance and conductance was studied.

  10. Accurately control and flatten gain spectrum of L-band erbium doped fiber amplifier based on suitable gain-clamping

    Yang, Jiuru; Meng, Xiangyu; Liu, Chunyu


    The increasing traffic with dynamic nature requires the applications of gain-clamped L-band erbium-doped fiber amplifier (EDFA). However, the weak or over clamping may lead the unexpected gain-compression and flatness-worsening. In this article, to enhance practicality, we modify the partly gain-clamping configuration and utilize a pair of C-band fiber Bragg gratings (FBGs) to non-uniformly compress the gain spectrum of L-band. Through a comprehensive test and comparison, the suitable gain-clamping region for the amplified signals is found, and the gain in L-band is accurately controlled and flattened under the matched central wavelength of FBGs. The experimental results show that, our designed L-band EDFA achieves a trade-off among the output gain, flatness and stability. The ±0.44 dB flatness and 20.2 dB average gain are together obtained in the range of 1570-1610 nm, with the ±0.1 dB stability of signals in over 30 dBm dynamic range.

  11. The Circuit-Level Decoupling Modulation Strategy for Three-Level Neutral-Point-Clamped (TL-NPC) Inverter

    Zhang, Zhe; Thomsen, Ole Cornelius; Andersen, Michael A. E.


    In this paper, a circuit-level decoupling modulation strategy is proposed for the three-level (TL) neutral-point-clamped (NPC) inverters. With the proposed modulation scheme, the TL-NPC inverter can be decoupled into two three-level Buck converters in each defined operating section, which makes...... without any feedback control. The simulation results show the validity of theoretical analysis....

  12. The application of the Escherichia coli giant spheroplast for drug screening with automated planar patch clamp system

    Kyoko Kikuchi


    Full Text Available Kv2.1, the voltage-gated ion channel, is ubiquitously expressed in variety of tissues and dysfunction of this ion channel is responsible for multiple diseases. Electrophysiological properties of ion channels are so far characterized with eukaryotic cells using the manual patch clamp which requires skilful operators and expensive equipments. In this research, we created a simple and sensitive drug screen method using bacterial giant spheroplasts and the automated patch clamp which does not require special skills. We expressed a eukaryotic voltage-gated ion channel Kv2.1 in Escherichia coli using prokaryotic codon, and prepared giant spheroplasts large enough for the patch clamp. Human Kv2.1 currents were successfully recorded from giant spheroplasts with the automated system, and Kv2.1-expressed E. coli spheroplasts could steadily reacted to the dose–response assay with TEA and 4-AP. Collectively, our results indicate for the first time that the bacterial giant spheroplast can be applied for practical pharmaceutical assay using the automated patch clamp.

  13. Thermal and efficiency analysis of five-level multi-level clamped multilevel converter considering grid codes

    Ma, Ke; Muñoz-Aguilar, S.; Rodriguez, P.;


    In this paper a five-level Multi-Clamped Multilevel Converter (5L-MLC2) topology is analyzed based on a typical grid integration application for renewable energy generation system. The loss and thermal distribution of the power devices in different switching legs are investigated and illustrated ...

  14. Modulation Methods for Three-level Neutral-Point-Clamped Inverter Achieving Stress Redistribution under Moderate Modulation Index

    Ma, Ke; Blaabjerg, Frede


    This letter investigates the loss and thermal behaviors of a three-level neutral-point-clamped (3L-NPC) inverter undergoing moderate modulation index, which is typically presented during minor voltage sags of the power grid or speed changes of the electric machines. A series of new space vector m...

  15. Single-Carrier Modulation for Neutral-Point-Clamped Inverters in Three-Phase Transformerless Photovoltaic Systems

    Guo, Xiaoqiang; Cavalcanti, Marcelo C.; Farias, Alexandre M.;


    Modulation strategy is one of the most important issues for three-level neutral-point-clamped inverters in three-phase transformerless photovoltaic systems. A challenge for modulation is how to keep the common-mode voltages constant to reduce the leakage currents. A single-carrier modulation...

  16. Grade 3C open femur fractures with vascular repair in adults.

    Balci, Halil I; Saglam, Yavuz; Tunali, Onur; Akgul, Turgut; Aksoy, Murat; Dikici, Fatih


    Grade 3C open femur fractures are challenging injuries with higher rates of complications. This is a retrospective review of grade 3C open femur fractures with vascular repair between 2002 and 2012. Outcomes included initial MESS score, additional injuries, duration of operation, complications, secondary operations or amputations, and social life implications. Thirty-one of 39 total patients were selected for revascularization and fracture fixation based on soft tissue injury and MESS score. The intra-operative approach included temporary arterial shunt replacement, orthopedic fixation, arterial reconstruction venous and/or nerve repair and routine fasciotomies. An external fixation and reverse saphenous vein graft was used in a majority of the patients (respectively; 93.5%, 90.3%). The mean follow up was 5.4 years (range 2.2-10). The decision to amputate versus salvage should be left up to patients and their care teams after discussing options and future possibilities rather than using a scoring system.

  17. 基于S3C2440的触摸屏驱动设计



    为了方便客户操作FTTH光纤熔接机,将触摸屏应用于此产品,分析了电阻式触摸屏的硬件结构和工作原理,探讨了基于S3C2440的触摸屏开发流程,设计了触摸屏的驱动程序,并改进了校准算法,提高了熔接机的响应速度和触摸手感。%The touch screen is used in FTTH fiber splice to operate it easily, analyse the structure of touch screen and working theory, discuss the develop procedure of touch screen based on S3C2440 , design the drive program of touch screen, improve the calibration arithmetic, improve the respond speed and touch feeling of splice.

  18. Soft X-ray Variability of the Bright Quasar 3C273

    Chulhee Kim


    We present the results from ROSAT observations of 3C273 in the soft X-ray band. The light variation of 3C273 was investigated for three different energy bands of soft, medium, and hard. The maximum variability with a factor of 2 for 551 dayswas confirmed at all three different bands. This appears to be a periodic variation within the period of roughly 6 months. However, the short-term or micro variation was not so distinct and the light variation of each band did not show any correlation between them. The hardness ratio for hard and soft bands shows irregular variation but there was no correlation between them. There is no distinct variation of the photon index in the case of simple power law model fitting. For power law + free absorption model fitting, the average photon index () is 2.08.

  19. Synchrotron radiation photoemission spectrum study on K3C60 film

    李宏年; 徐亚伯; 鲍世宁; 李海洋; 何丕模; 钱海杰; 刘风琴; 奎热西·易卜拉欣


    K3C60 single crystal film was prepared on the cleaved (111) surface of C60 single crystal. Synchrotron radiation angle-resolved photoemission spectra were measured at normal emission with sample temperature at - 150K. Up to four subpeaks of LUMO-derived band were observed. These sub-peaks exhibit distinct energy dispersions which resemble in general the theoretical ones calculated for K3C60 at low temperature with the so-called one-dimensional disordered structure. But there is large deviation of experimental sub-band intervals from the theoretical values. This result is meaningful for the studies of the physical properties of alkali-doped C60 solids, e.g. the mechanism for superconductivity.

  20. Improved real-time dosimetry using the radioluminescence signal from Al2O3:C

    Damkjær, Sidsel Marie Skov; Andersen, Claus Erik; Aznar, Marianne


    15th International Conference on Solid State Dosimetry Location: Delft Univ Technol, Delft, NETHERLANDS Date: JUL 08-13, 2007 Abstract: Carbon-doped aluminum oxide (Al2O3:C) is a highly sensitive luminescence material for ionizing radiation dosimetry, and it is well established that the optically...... to greatly reduce the influence of shallow traps in the range from 0 to 3 Gy and the RL dose-rate measurements with a time resolution of 0. 1 s closely matched dose-rate changes monitored with in ionization chamber. (c) 2007 Elsevier Ltd. All rights reserved.......15th International Conference on Solid State Dosimetry Location: Delft Univ Technol, Delft, NETHERLANDS Date: JUL 08-13, 2007 Abstract: Carbon-doped aluminum oxide (Al2O3:C) is a highly sensitive luminescence material for ionizing radiation dosimetry, and it is well established that the optically...