Sample records for 2p 3p 5q

  1. Doubly excited 2s2p 1,3p1 resonances in photoionization of helium

    Wan Jian-Jie; Dong Chen-Zhong


    The multi-configuration Dirac-Fock (MCDF) method is implemented to study doubly excited 2s2p 1,3P1 resonances of the helium atom and the interference between photoionization and photoexcitation autoionization processes.In order to reproduce the total photoionization sprectra,the excited energies from the ground ls2 1 S0 state to the doubly excited 2s2p 1'3P1 states and the relevant Auger decay rates and widths are calculated in detail. Furthermore,the interference profile determined by the so-called Fano parameters q and p2 is also reproduced. Good agreement is found between the present results and other available theoretical and experimental results. This indeed shows a promising way to investigate the Fano resonances in photoionization of atoms within the MCDF scheme,although there are some discrepancies in the present calculations of the 2s2p 3P1 state.

  2. Unified interpretation of Hund's first and second rules for 2p and 3p atoms.

    Oyamada, Takayuki; Hongo, Kenta; Kawazoe, Yoshiyuki; Yasuhara, Hiroshi


    A unified interpretation of Hund's first and second rules for 2p (C, N, O) and 3p (Si, P, S) atoms is given by Hartree-Fock (HF) and multiconfiguration Hartree-Fock (MCHF) methods. Both methods exactly satisfy the virial theorem, in principle, which enables one to analyze individual components of the total energy E(=T+V(en)+V(ee)), where T, V(en), and V(ee) are the kinetic, the electron-nucleus attraction, and the electron-electron repulsion energies, respectively. The correct interpretation for each of the two rules can only be achieved under the condition of the virial theorem 2T+V=0 by investigating how V(en) and V(ee) interplay to attain the lower total potential energy V(=V(en)+V(ee)). The stabilization of the more stable states for all the 2p and 3p atoms is ascribed to a greater V(en) that is caused by contraction of the valence orbitals accompanied with slight expansion of the core orbitals. The contraction of the valence orbitals for the two rules is a consequence of reducing the Hartree screening of the nucleus at short interelectronic distances. The reduced screening in the first rule is due to a greater amount of Fermi hole contributions in the state with the highest total spin-angular momentum S. The reduced screening in the second rule is due to the fact that two valence electrons are more likely to be on opposite sides of the nucleus in the state with the highest total orbital-angular momentum L. For each of the two rules, the inclusion of correlation does not qualitatively change the HF interpretation, but HF overestimates the energy difference ∣ΔE∣ between two levels being compared. The magnitude of the correlation energy is significantly larger for the lower L states than for the higher L states since two valence electrons in the lower L states are less likely to be on opposite sides of the nucleus. The MCHF evaluation of ∣ΔE∣ is in excellent agreement with experiment. The present HF and MCHF calculations demonstrate the above statements

  3. Phospholipid flippases Lem3p-Dnf1p and Lem3p-Dnf2p are involved in the sorting of the tryptophan permease Tat2p in yeast.

    Hachiro, Takeru; Yamamoto, Takaharu; Nakano, Kenji; Tanaka, Kazuma


    The type 4 P-type ATPases are flippases that generate phospholipid asymmetry in membranes. In budding yeast, heteromeric flippases, including Lem3p-Dnf1p and Lem3p-Dnf2p, translocate phospholipids to the cytoplasmic leaflet of membranes. Here, we report that Lem3p-Dnf1/2p are involved in transport of the tryptophan permease Tat2p to the plasma membrane. The lem3Δ mutant exhibited a tryptophan requirement due to the mislocalization of Tat2p to intracellular membranes. Tat2p was relocalized to the plasma membrane when trans-Golgi network (TGN)-to-endosome transport was inhibited. Inhibition of ubiquitination by mutations in ubiquitination machinery also rerouted Tat2p to the plasma membrane. Lem3p-Dnf1/2p are localized to endosomal/TGN membranes in addition to the plasma membrane. Endocytosis mutants, in which Lem3p-Dnf1/2p are sequestered to the plasma membrane, also exhibited the ubiquitination-dependent missorting of Tat2p. These results suggest that Tat2p is ubiquitinated at the TGN and missorted to the vacuolar pathway in the lem3Δ mutant. The NH(2)-terminal cytoplasmic region of Tat2p containing ubiquitination acceptor lysines interacted with liposomes containing acidic phospholipids, including phosphatidylserine. This interaction was abrogated by alanine substitution mutations in the basic amino acids downstream of the ubiquitination sites. Interestingly, a mutant Tat2p containing these substitutions was missorted in a ubiquitination-dependent manner. We propose the following model based on these results; Tat2p is not ubiquitinated when the NH(2)-terminal region is bound to membrane phospholipids, but if it dissociates from the membrane due to a low level of phosphatidylserine caused by perturbation of phospholipid asymmetry in the lem3Δ mutant, Tat2p is ubiquitinated and then transported from the TGN to the vacuole.

  4. Reactions of R(2)P-P(SiMe(3))Li with [(R'(3)P)(2)PtCl(2)]. A general and efficient entry to phosphanylphosphinidene complexes of platinum. Syntheses and structures of [(eta(2)-P=(i)Pr(2))Pt(p-Tol(3)P)(2)], [(eta(2)-P=(t)Bu(2))Pt(p-Tol(3)P)(2)], [{eta(2)-P=(N(i)Pr(2))(2)}Pt(p-Tol(3)P)(2)] and [{(Et(2)PhP)(2)Pt}(2)P(2)].

    Domańska-Babul, Wioleta; Chojnacki, Jaroslaw; Matern, Eberhard; Pikies, Jerzy


    The reactions of lithium derivatives of diphosphanes R(2)P-P(SiMe(3))Li (R = (t)Bu, (i)Pr, Et(2)N and (i)Pr(2)N) with [(R'(3)P)(2)PtCl(2)] (R'(3)P = Et(3)P, Et(2)PhP, EtPh(2)P and p-Tol(3)P) proceed in a facile manner to afford side-on bonded phosphanylphosphinidene complexes of platinum [(eta(2)-P=R(2))Pt(PR'(3))(2)]. The related reactions of Ph(2)P-P(SiMe(3))Li with [(R'(3)P)(2)PtCl(2)] did not yield [(eta(2)-P=PPh(2))Pt(PR'(3))(2)] and resulted mainly in the formation of [{(R'(3)P)(2)Pt}(2)P(2)], Ph(2)P-PLi-PPh(2), (Me(3)Si)(2)PLi and (Me(3)Si)(3)P. Crystallographic data are reported for the compounds [(eta(2)-P=R(2))Pt(p-Tol(3)P)(2)] (R = (t)Bu, (i)Pr, ((i)Pr(2)N)(2)P) and for [{(Et(2)PhP)(2)Pt}(2)P(2)].

  5. Identification of a rare de novo three-way complex t(5;20;8(q31;p11.2;p21 with microdeletions on 5q31.2, 5q31.3, and 8p23.2 in a patient with hearing loss and global developmental delay: case report

    Bejjani Bassem A


    Full Text Available Abstract Background Complex chromosome rearrangements (CCRs, which involve more than two breakpoints on two or more chromosomes, are uncommon occurrences. Although most CCRs appear balanced at the level of the light microscope, many demonstrate cryptic, submicroscopic imbalances at the translocation breakpoints. Results We report a female with hearing loss and global developmental delay with a complex three-way unbalanced translocation (5;20;8(q31;p11.2;p21 resulting in microdeletions on 5q31.2, 5q31.3, and 8p23.2 identified by karyotyping, microarray analysis and fluorescence in situ hybridization. Discussion The microdeletion of bands 8p23.2 may be associated with the hearing impairment. Furthermore, the characterization of this patient's chromosomal abnormalities demonstrates the importance of integrated technologies within contemporary cytogenetics laboratories.

  6. The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo.

    Brown, J. T.; Bai, X.; Johnson, A. W.


    The yeast superkiller (SKI) genes were originally identified from mutations allowing increased production of killer toxin encoded by M "killer" virus, a satellite of the dsRNA virus L-A. XRN1 (SKI1) encodes a cytoplasmic 5'-exoribonuclease responsible for the majority of cytoplasmic RNA turnover, whereas SKI2, SKI3, and SKI8 are required for normal 3'-degradation of mRNA and for repression of translation of poly(A) minus RNA. Ski2p is a putative RNA helicase, Ski3p is a tetratricopeptide repe...

  7. Yeast Agp2p and Agp3p function as amino acid permeases in poor nutrient conditions.

    Schreve, James L; Garrett, Jinnie M


    The gene AGP2 and the ORF YFL055w (here named AGP3) are classified as members of the yeast amino acid permease gene family. Analysis of the growth of multiply-mutant strains in which these genes are disrupted shows that both encode permeases capable of supplying branched chain, and other, amino acids as nitrogen source. Both Agp2p and Agp3p are low affinity permeases for leucine (Kmapp 0.2-0.5 mM) and are expressed at lower levels than other permeases on all media tested. Thus, it appears that these two permeases can function as low affinity, relatively non-specific, permeases with redundant functions in the cell. Transcription of AGP2 and AGP3 is very low but is increased in cells lacking other functional general amino acid permeases (Gap1p or Agp1p). These results suggest Agp2p and Agp3p function in amino acid transport when nitrogen sources are limiting and/or other permeases are inactive.

  8. 75 FR 23574 - Airworthiness Directives; CFM International, S.A. CFM56-5B1/P, -5B2/P, -5B3/P, -5B3/P1, -5B4/P...


    ..., S.A. CFM56-5B1/P, - 5B2/P, -5B3/P, -5B3/P1, -5B4/P, -5B5/P, -5B6/P, -5B7/P, -5B8/P, -5B9/P, -5B1/2P... Docket Operations office between 9 a.m. and 5 p.m., Monday through Friday, except Federal holidays. The... CFM International, S.A. CFM56-5B1/P, - 5B2/P, -5B3/P, -5B3/P1, -5B4/P, -5B5/P,......

  9. Photoexcitation and photoionization from the 2p{sup 5}3p[5/2]{sub 2,3} levels in neon

    Baig, M. A.; Bokhari, I. A.; Rafiq, M.; Kalyar, M. A.; Hussian, T.; Ali, Raheel; Piracha, N. K. [Atomic and Molecular Physics Laboratory, Department of Physics, Quaid-i-Azam University, 45320 Islamabad (Pakistan); Department of Physics, John Carroll University, 20700 N. Park Boulevard, University Heights, Ohio 44118 (United States)


    We present measurements of the excitation spectra from the 2p{sup 5}3p[5/2]{sub 3,2} levels in neon using two-step laser excitation and ionization in conjunction with an optogalvanic detection in dc and rf discharge cells. The 2p{sup 5}3p[5/2]{sub 3,2} intermediate levels have been approached via the collisionally populated 2p{sup 5}3s[3/2]{sub 2} metastable level. The Rydberg series 2p{sup 5}({sup 2}P{sub 3/2})nd[7/2]{sub 4} (12 {<=}n{<=} 44), 2p{sup 5}({sup 2}P{sub 3/2})ns[3/2]{sub 2} (13 {<=}n{<=} 35) and the parity forbidden transitions 2p{sup 5}({sup 2}P{sub 3/2})np[5/2]{sub 3} (13 {<=}n{<=} 19) have been observed from the 2p{sup 5}3p[5/2]{sub 3} level, whereas the 2p{sup 5}({sup 2}P{sub 3/2})nd[7/2]{sub 3} (12 {<=}n{<=} 44), 2p{sup 5}({sup 2}P{sub 3/2})ns[3/2]{sub 2} (13 {<=}n{<=} 35), and 2p{sup 5}({sup 2}P{sub 1/2})nd'[5/2]{sub 3} (9 {<=}n{<=} 12) Rydberg series have been observed from the 2p{sup 5}3p[5/2]{sub 2} level in accordance with the {Delta}J ={Delta}K = {+-} 1 selection rules. The photoionization cross sections from the 2p{sup 5}3p[5/2]{sub 3} intermediate level have been measured at eight ionizing laser wavelengths (399, 395, 390, 385, 380, 370, 364, and 355 nm) and that from the 2p{sup 5}3p[5/2]{sub 2} level at 401.8 nm. These measurements are in excellent agreement with the experimental values reported in the literature, while the experimental data lie much below the theoretically calculated photoionization cross sections curve.

  10. Correlation and relativistic effects in 2p photoelectron spectra of sodium atoms from the initial state 2{p}^{6}3p

    Liu, Xiaobin; Shi, Yinglong; Xing, Yongzhong; Lu, Feiping; Chen, Zhanbin


    We investigate the 2p photoelectron spectra of sodium atoms with the initial state 2{p}63p at a photon energy of 54 eV. The analysis is performed based on the multi-configuration Dirac–Fock method. Special attention is given to the influences of correlation and relativistic effects on the spectra structures. To explore the nature and importance of such influences, calculations were performed based on detailed analyses of the thresholds, relative intensities and corresponding data calculated in the nonrelativistic limit.

  11. Yet1p-Yet3p interacts with Scs2p-Opi1p to regulate ER localization of the Opi1p repressor.

    Wilson, Joshua D; Thompson, Sarah L; Barlowe, Charles


    Lipid sensing mechanisms at the endoplasmic reticulum (ER) coordinate an array of biosynthetic pathways. A major phospholipid regulatory circuit in yeast is controlled by Scs2p, an ER membrane protein that binds the transcriptional repressor protein Opi1p. Cells grown in the absence of inositol sequester Scs2p-Opi1p at the ER and derepress target genes including INO1. We recently reported that Yet1p and Yet3p, the yeast homologues of BAP29 and BAP31, are required for normal growth in the absence of inositol. Here we show that the Yet1p-Yet3p complex acts in derepression of INO1 through physical association with Scs2p-Opi1p. Yet complex binding to Scs2p-Opi1p was enhanced by inositol starvation, although the interaction between Scs2p and Opi1p was not influenced by YET1 or YET3 deletion. Interestingly, live-cell imaging analysis indicated that Opi1p does not efficiently relocalize to the ER during inositol starvation in yet3Δ cells. Together our data demonstrate that a physical association between the Yet complex and Scs2p-Opi1p is required for proper localization of the Opi1p repressor to ER membranes and subsequent INO1 derepression.

  12. Measurement of the 1s2s 1S0-1s2p 3P1 intercombination interval in helium-like silicon.

    Redshaw, M; Myers, E G


    Using Doppler-tuned fast-beam laser spectroscopy the 1s2s 1S0-1s2p 3P1 intercombination interval in 28Si12+ has been measured to be 7230.5(2) cm(-1). The experiment made use of a single-frequency Nd:YAG (1.319 microm) laser and a high-finesse optical buildup cavity. The result provides a precision test of modern relativistic and QED atomic theory.

  13. Allelic variants of hexose transporter Hxt3p and hexokinases Hxk1p/Hxk2p in strains of Saccharomyces cerevisiae and interspecies hybrids.

    Zuchowska, Magdalena; Jaenicke, Elmar; König, Helmut; Claus, Harald


    The transport of sugars across the plasma membrane is a critical step in the utilization of glucose and fructose by Saccharomyces cerevisiae during must fermentations. Variations in the molecular structure of hexose transporters and kinases may affect the ability of wine yeast strains to finish sugar fermentation, even under stressful wine conditions. In this context, we sequenced and compared genes encoding the hexose transporter Hxt3p and the kinases Hxk1p/Hxk2p of Saccharomyces strains and interspecies hybrids with different industrial usages and regional backgrounds. The Hxt3p primary structure varied in a small set of amino acids, which characterized robust yeast strains used for the production of sparkling wine or to restart stuck fermentations. In addition, interspecies hybrid strains, previously isolated at the end of spontaneous fermentations, revealed a common amino acid signature. The location and potential influence of the amino acids exchanges is discussed by means of a first modelled Hxt3p structure. In comparison, hexokinase genes were more conserved in different Saccharomyces strains and hybrids. Thus, molecular variants of the hexose carrier Hxt3p, but not of kinases, correlate with different fermentation performances of yeast.

  14. Evaluation of electron population terms for 4p, 3p, and (2p): how do HOMO and LUMO shrink or expand depending on nuclear charges?

    Nakanishi, Waro; Hayashi, Satoko; Narahara, Kenji; Yamaki, Daisuke; Hada, Masahiko


    Electron population terms are evaluated for N=Se, S, and O. Calculations are performed on HOMO and LUMO constructed by pure atomic 4p(Se), 3p(S), and 2p(O) orbitals, employing the 6-311+G(3d) and/or 6-311(++)G(3df,3pd) basis sets at the HF, MP2, and DFT (B3 LYP) levels. Se(4+), Se(2+), Se(0), and Se(2-) with the O(h) symmetry are called G(A: Se) and HSe(+), H(2)Se, and HSe(-) with the C(infinityh) or C(2v) symmetry are named G(B: Se), here [G(A+B: Se) in all]. HOMO and LUMO in G(A+B: N) (N=Se, S, and O) satisfy the conditions of the calculations for . The (4p), (3p), and (2p) values correlate well with the corresponding MO energies (epsilon(N)) for all calculation levels employed. Plots of (HOMO) and (LUMO) versus Q(N) (N=Se, S, and O) at the HF and MP2 levels are analyzed as two correlations. However, the plots at the DFT level can be analyzed as single correlation. A regression curve is assumed for the analysis. Behaviors of clarify how valence orbitals shrink or expand depending on Q(N). The applicability of is examined to establish a new method that enables us to analyze chemical shifts with the charge effect separately from others. A utility program derived from the Gaussian 03 (NMRANAL-NH03G) is applied to evaluate and examine the applicability to the NMR analysis.

  15. Simultaneous measurements of photoionization cross-sections of lithium isotopes from 3p {sup 2}P{sub 1/2, 3/2}

    Saleem, M; Hussain, Shahid; Rafiq, M; Baig, M A [Atomic and Molecular Physics Laboratory, Department of Physics, Quaid-i-Azam University, 45320 Islamabad (Pakistan)


    We present the first measurements of the photoionization cross-section of the 3p {sup 2}P{sub 1/2, 3/2} excited states for both the lithium isotopes Li{sup 6} and Li{sup 7} in the region close to the Li{sup +} 1s{sup 2 1}S threshold (from 0 to 3 eV) using the two-step photoionization and the saturation technique. Narrow linewidth dye lasers have been used in combination with a locally developed atomic beam-TOF mass spectrometer to measure the photoion signals as a function of energy density of the ionizing laser. It is observed that the cross-section is maximum near the ionization threshold and decreases monotonically as the excess photon energy of the ionizing laser is increased. The deduced number densities of the isotopes are Li{sup 6} (1.2 x 10{sup 8} atoms cm{sup -3}) and Li{sup 7} (1.2 x 10{sup 9} atoms cm{sup -3}), which are found to be close to their natural abundance ratio within the experimental error. Our experimental results of the measurement of the cross-section are also in good agreement with earlier theoretical work.


    Andrea Pellagatti


    Full Text Available The 5q- syndrome is the most distinct of the myelodysplastic syndromes (MDS and patients with this disorder have a deletion of chromosome 5q [del(5q] as the sole karyotypic abnormality. Several genes mapping to the commonly deleted region of the 5q- syndrome have been implicated in disease pathogenesis in recent years. Haploinsufficiency of the ribosomal gene RPS14 has been shown to cause the erythroid defect in the 5q- syndrome. Loss of the microRNA genes miR-145 and miR-146a has been associated with the thrombocytosis observed in 5q- syndrome patients. Haploinsufficiency of CSNK1A1 leads to hematopoietic stem cell expansion in mice and may play a role in the initial clonal expansion in patients with 5q- syndrome. Moreover, a subset of patients harbor mutation of the remaining CSNK1A1 allele. Mouse models of the 5q- syndrome, which recapitulate the key features of the human disease, indicate that a p53-dependent mechanism underlies the pathophysiology of this disorder. Importantly, activation of p53 has been demonstrated in the human 5q- syndrome. Recurrent TP53 mutations have been associated with an increased risk of disease evolution and with decreased response to the drug lenalidomide in del(5q MDS patients. Potential new therapeutic agents for del(5q MDS include the translation enhancer L-leucine.

  17. Synthesis and characterization of fac-[M(CO)3(P)(OO)] and cis-trans-[M(CO)2(P)2(OO)] complexes (M = Re, (99m)Tc) with acetylacetone and curcumin as OO donor bidentate ligands.

    Triantis, Charalampos; Tsotakos, Theodoros; Tsoukalas, Charalampos; Sagnou, Marina; Raptopoulou, Catherine; Terzis, Aris; Psycharis, Vassilis; Pelecanou, Maria; Pirmettis, Ioannis; Papadopoulos, Minas


    The synthesis and characterization of neutral mixed ligand complexes fac-[M(CO)3(P)(OO)] and cis-trans-[M(CO)2(P)2(OO)] (M = Re, (99m)Tc), with deprotonated acetylacetone or curcumin as the OO donor bidentate ligands and a phosphine (triphenylphosphine or methyldiphenylphosphine) as the monodentate P ligand, is described. The complexes were synthesized through the corresponding fac-[M(CO)3(H2O)(OO)] (M = Re, (99m)Tc) intermediate aqua complex. In the presence of phosphine, replacement of the H2O molecule of the intermediate complex at room temperature generates the neutral tricarbonyl monophosphine fac-[Re(CO)3(P)(OO)] complex, while under reflux conditions further replacement of the trans to the phosphine carbonyl generates the new stable dicarbonyl bisphosphine complex cis-trans-[Re(CO)2(P)2(OO)]. The Re complexes were fully characterized by elemental analysis, spectroscopic methods, and X-ray crystallography showing a distorted octahedral geometry around Re. Both the monophosphine and the bisphosphine complexes of curcumin show selective binding to β-amyloid plaques of Alzheimer's disease. At the (99m)Tc tracer level, the same type of complexes, fac-[(99m)Tc(CO)3(P)(OO)] and cis-trans-[(99m)Tc(CO)2(P)2(OO)], are formed introducing new donor combinations for (99m)Tc(I). Overall, β-diketonate and phosphine constitute a versatile ligand combination for Re(I) and (99m)Tc(I), and the successful employment of the multipotent curcumin as β-diketone provides a solid example of the pharmacological potential of this system.

  18. Genetics Home Reference: 5q minus syndrome

    ... for ribosomal protein genes causes selective activation of p53 in human erythroid progenitor cells. Blood. 2011 Mar ... U, List A, MacBeth KJ. Lenalidomide as a disease-modifying agent in patients with del(5q) myelodysplastic ...

  19. Relativistic evaluation of the two-photon decay of the metastable 1 s22 s 2 p 3P0 state in berylliumlike ions with an effective-potential model

    Amaro, Pedro; Fratini, Filippo; Safari, Laleh; Machado, Jorge; Guerra, Mauro; Indelicato, Paul; Santos, José Paulo


    The two-photon 1 s22 s 2 p 3P0→1 s2s21S0 transition in berylliumlike ions is investigated theoretically within a fully relativistic framework and a second-order perturbation theory. We focus our analysis on how electron correlation, as well as the negative-energy spectrum, can affect the forbidden E 1 M 1 decay rate. For this purpose, we include the electronic correlation via an effective local potential and within a single-configuration-state model. Due to its experimental interest, evaluations of decay rates are performed for berylliumlike xenon and uranium. We find that the negative-energy contribution can be neglected at the present level of accuracy in the evaluation of the decay rate. On the other hand, if contributions of electronic correlation are not carefully taken into account, it may change the lifetime of the metastable state by up to 20%. By performing a fully relativistic j j -coupling calculation, we find a decrease of the decay rate by two orders of magnitude compared to nonrelativistic L S -coupling calculations, for the selected heavy ions.

  20. Alignment of Ar{sup +} [{sup 3}P]4p{sup 2}P{sup 0}{sub 3/2} satellite state from the polarization analysis of fluorescent radiation after photoionization

    Yenen, O.; McLaughlin, K.W.; Jaecks, D.H. [Univ. of Nebraska, Lincoln, NE (United States)] [and others


    The measurement of the polarization of radiation from satellite states of Ar{sup +} formed after the photoionization of Ar provides detailed information about the nature of doubly excited states, magnetic sublevel cross sections and partial wave ratios of the photo-ejected electrons. Since the formation of these satellite states is a weak process, it is necessary to use a high flux beam of incoming photons. In addition, in order to resolve the many narrow doubly excited Ar resonances, the incoming photons must have a high resolution. The characteristics of the beam line 9.0.1 of the Advanced Light Source fulfill these requirements. The authors determined the polarization of 4765 {Angstrom} fluorescence from the Ar{sup +} [{sup 3}P] 4p {sup 2}P{sub 3/2}{sup 0} satellite state formed after photoionization of Ar by photons from the 9.0.1 beam line of ALS in the 35.620-38.261 eV energy range using a resolution of approximately 12,700. This is accomplished by measuring the intensities of the fluorescent light polarized parallel (I{parallel}) and perpendicular (I{perpendicular}) to the polarization axis of the incident synchrotron radiation using a Sterling Optics 105MB polarizing filter. The optical system placed at 90{degrees} with respect to the polarization axis of the incident light had a narrow band interference filter ({delta}{lambda}=0.3 nm) to isolate the fluorescent radiation.

  1. Main: 1B5Q [RPSD[Archive


  2. Calculation of the total electron excitation cross section in the Born approximation using Slater wave functions for the Li (2s yields 2p), Li (2s yields 3p), Na (3s yields 4p), Mg (3p yields 4s), Ca (4s yields 4p) and K (4s yields 4p) excitations. M.S. Thesis

    Simsic, P. L.


    Excitation of neutral atoms by inelastic scattering of incident electrons in gaseous nebulae were investigated using Slater Wave functions to describe the initial and final states of the atom. Total cross sections using the Born Approximation are calculated for: Li(2s yields 2p), Na(3s yields 4p), k(4s yields 4p). The intensity of emitted radiation from gaseous nebulae is also calculated, and Maxwell distribution is employed to average the kinetic energy of electrons.

  3. A locus regulating bronchial hyperresponsiveness maps to chromosome 5q

    Levitt, R.C.; Meyers, D.A. [Johns Hopkins Medical Institutions, Baltimore, MD (United States); Bleecker, E.R. [Univ. of Maryland, Baltimore, MD (United States)] [and others


    Bronchial hyperresponsiveness (BHR) is one of the hallmarks of asthma. BHR correlates well with asthmatic symptoms and the response to treatment. Moreover, BHR appears to be closely related to airways inflammation. Numerous studies have demonstrated a familial aggregation; however, this phenotype is not likely inherited as a simple Mendelian trait. BHR is also closely associated with total serum IgE levels, as are allergy and asthma. We studied 92 families from Northern Holland ascertained through a parent with asthma who were originally studied between 1962-1970. Since there are a number of candidate genes on chromosome 5q potentially important in producing BHR, families were genotyped for markers in this region. These genes regulate IgE production and the cellular elements that are likely involved in inflammation associated with BHR, allergy and asthma. They include IL-4, IL-3, IL-5, IL-9, IL-12, IL-13 and GM-CSF. Linkage of BHR with markers on 5q was tested using a model free sib-pair method. The data suggest a locus for BHR maps near the cytokine gene cluster on 5q. This region appears critical in producing susceptibility to BHR and possibly to asthma.

  4. Síndrome 5q-: um relato de caso

    Marcelo Gil Cliquet


    Full Text Available As síndromes mielodisplásicas (SMD se caracterizam por hematopoiese ineficaz, citopenias, distúrbios qualitativos de uma ou mais linhagens de células do sangue periférico, anormalidades cromossômicas e uma predileção variável de evolução para leucemias mielóides agudas. As anormalidades mais freqüentemente observadas incluem a deleção do braço longo do cromossomo 5 (5q-. O tratamento com novas drogas vem mudando as perspectivas de tratamento. A lenalidomida é um fármaco que fica reservada para o tratamento das síndromes mielodisplásicas 5q- e mieloma múltiplo. Objetivo: Relatar o uso do fármaco lenalidomida por um paciente portador de SMD com a síndrome 5q-, avaliando o impacto na necessidade transfusional. Relato de caso: Paciente de 64 anos, sexo masculino, encaminhado por apresentar anemia (8,2g/dl macrocítica (VCM=109fl. Série branca e plaquetária normais. Após investigação para hemólise, carência de B12, ácido fólico e Ferro sem anormalidades, foi solicitada avaliação de medula óssea que revelou hipercelularidade (relação G/E=4/1, aumento de mieloblastos e prómielocitos, e cariótipo mostrando 46XY del(5(q15q33{6} e 46XY{14}. Prescrito Lenalidomida (10mg VO/dia em ciclos de 21 dias com intervalo de uma semana. Desde a primeira consulta até o início do tratamento, o paciente recebeu 16 unidades de concentrados de hemácias. Após dois ciclos, observou-se elevação da hemoglobina, sem transfusões, chegando a 12,3g/dl. Apresentou, no entanto, redução de neutrófilos (970/mm³ e de plaquetas (107.000/mm3. Conclusão: O uso do fármaco pode melhorar a qualidade de vida e a sobrevida dos pacientes portadores da síndrome 5q-, tendo em vista a redução de transfusões de sangue, evitando, assim, a sobrecarga de ferro e ainda pode diminuir a taxa de transformação em LMA.

  5. SPIN-FORBIDDEN TRANSITIONS FOR BE-LIKE IONS (Z=10-103)%高离化类铍离子2s2 1S0-2s2p3P1(Z=10-103)自旋禁戒光谱跃迁

    易有根; 汪蓉; 李向东; 王红艳; 朱正和


    利用全相对论性多组态Dirac-Fock平均能级(MCDF-AL)方法系统地计算了高离化类铍离子自旋禁戒2s2 1S0-2s2p3P1(Z=10-103)跃迁的能级间隔和跃迁概率,计算中考虑了重要的核的有限体积效应,Breit修正和QED修正,所得结果和最近的实验数据及理论计算值进行了比较,结果表明:高原子序数的高荷电离子的自旋禁戒跃迁的跃迁概率和中性原子的电偶极E1的相当,在ICF和MCF高温激光等离子体中,自旋禁戒跃迁概率过程不容被忽视.

  6. On the kinetic and thermodynamic fragility of the Pt{sub 60}Cu{sub 16}Co{sub 2}P{sub 22} and Pt{sub 57.3}Cu{sub 14.6}Ni{sub 5.3}P{sub 22.8} bulk metallic glasses

    Gallino, Isabella, E-mail: [Saarland University, Department of Materials Science and Engineering, Campus C6.3, 66123 Saarbruecken (Germany); Gross, Oliver [Saarland University, Department of Materials Science and Engineering, Campus C6.3, 66123 Saarbruecken (Germany); Dalla Fontana, Giulia [Department of Chemistry IFM and NIS, University of Torino, V. Giuria 7, 10125 Torino (Italy); Evenson, Zach; Busch, Ralf [Saarland University, Department of Materials Science and Engineering, Campus C6.3, 66123 Saarbruecken (Germany)


    Highlights: • The studied Pt–Cu–(Ni,Co)–P glasses are more fragile than Zr-based alloys. • They show large increases in the C{sub p} at T{sub g} and small barriers for cooperative rearrangements of atoms. • They have fragility parameters among the lowest reported for BMG systems (D{sup *} = 10–12). • They crystallize into a state that melts with distinctly high entropy of fusion. • The microscopic origin of their fragility seems different than that for Zr- and Pd-based BMGs. - Abstract: The investigations in this study focus on bulk metallic glass (BMG) alloy families based on noble metals like Pt, which are more kinetically fragile than Zr-based BMG systems. Thermophysical properties have been investigated by calorimetry and thermal mechanical analyses for the determination of the specific heat capacity and viscosity, respectively. For the Pt{sub 60}Cu{sub 16}Co{sub 2}P{sub 22} and Pt{sub 57.3}Cu{sub 14.6}Ni{sub 5.3}P{sub 22.8} BMG compositions consistent Vogel–Fulcher–Tammann (VFT) fits of the viscosity measurements are established, and the temperature dependence of the configurational entropy is calculated from thermodynamic data. Fits to the Adam–Gibbs equation are performed using this configurational entropy change. Their fragile nature is compared to that of Zr-based alloys in terms of structural considerations.

  7. Síndrome 5q-: um relato de caso

    Marcelo Gil Cliquet; Fábio Moreira Campos; Gabriel Murad Takao; Ricardo Hiroshi Murashita Fujiki; Thays Brunelli Pugliesi; Guilherme Cliquet


    As síndromes mielodisplásicas (SMD) se caracterizam por hematopoiese ineficaz, citopenias, distúrbios qualitativos de uma ou mais linhagens de células do sangue periférico, anormalidades cromossômicas e uma predileção variável de evolução para leucemias mielóides agudas. As anormalidades mais freqüentemente observadas incluem a deleção do braço longo do cromossomo 5 (5q-). O tratamento com novas drogas vem mudando as perspectivas de tratamento. A lenalidomida é um fármaco que fica reservada p...

  8. PP2A: The Achilles Heal in MDS with 5q Deletion

    David eSallman


    Full Text Available Myelodysplastic syndromes (MDS represent a hematologically diverse group of myeloid neoplasms, however, one subtype characterized by an isolated deletion of chromosome 5q (del(5q is pathologically and clinically distinct. Patients with del(5q MDS share biological features that account for the profound hypoplastic anemia and unique sensitivity to treatment with lenalidomide. Ineffective erythropoiesis in del(5q MDS arises from allelic deletion of the ribosomal processing S-14 (RPS14 gene, which leads to MDM2 sequestration with consequent p53 activation and erythroid cell death. Since its approval in 2005, lenalidomide has changed the natural course of the disease. Patients who achieve transfusion independence and/or a cytogenetic response with lenalidomide have a decreased risk of progression to AML and an improved overall survival compared to non-responders. Elucidation of the mechanisms of action of lenalidomide in del(5q MDS has advanced therapeutic strategies for this disease. The selective cytotoxicity of lenalidomide in del(5q clones derives from inhibition of a haplodeficient phosphatase whose catalytic domain is encoded within the common deleted region on chromosome 5q, i.e., protein phosphatase 2A (PP2Acα. PP2A is a highly conserved, dual specificity phosphatase that plays an essential role in regulation of the G2/M checkpoint. Inhibition of PP2Acα results in cell cycle arrest and apoptosis in del(5q cells. Targeted knockdown of PP2Acα using siRNA is sufficient to sensitize non-del(5q clones to lenalidomide. Through its inhibitory effect on PP2A, lenalidomide stabilizes MDM2 to restore p53 degradation in erythroid precursors, with subsequent arrest in G2/M. Unfortunately, the majority of patients with del(5q MDS develop resistance to lenalidomide over time associated with PP2Acα overexpression. Targeted inhibition of PP2A with a more potent inhibitor has emerged as an attractive therapeutic approach for patients with del(5q MDS.

  9. Saperi P2P

    Salvatore Iaconesi


    Full Text Available Il paper presenta l'architettura filosofica e logica di un progetto ongoing per la creazione di un'infrastruttura peer to peer per la diffusione dei saperi. Tale infrastruttura p2p vuole essere la base per costruire un framework aperto e orizzontale, che ospiti pratiche innovative di creazione, condivisione e disseminazione di informazioni e conoscenza.

  10. Partial trisomy 5q resulting from chromosome 7 insertion: An expansion of the phenotype

    Fries, M.H.; Reilly, P.A.; Williams, T.C. [Keesler Medical Center, MS (United States)] [and others


    Partial trisomy 5q has been categorized into three separate phenotypes; however, a distinctive phenotype has not been described for duplications spanning 5q23-q35. We report a case of partial trisomy 5q for this region as a result of a ins(7,5)(q31.3;q23.2q35.1)mat. The liveborn male infant was delivered by emergency cesarean section at 37 weeks after a pregnancy notable for oligohydramnios, with birth weight 1792 g (<3%). Postnatal course was marked by psychomotor delay, failure to thrive, and biopsy demonstrated neonatal giant cell hepatitis with a paucity of intrahepatic bile ducts. His appearance was remarkable for lack of subcutaneous fat, midline displaced hair whorl, bitemporal narrowing with frontal bossing, wide anterior fontanel, widow`s peak, protuberant eyes with periorbital and lid edema, short flat nasal bridge with broad flattened nasal tip, long smooth philtrum, wide mouth with thin lips, wide gingival ridges, micrognathia, posteriorly rotated low-set ears, hepatomegaly, flexion contractions of elbows, and generalized hypertonicity. Urine organic acids, oligosaccharide/mucopolysaccharide screen, and plasma amino acids were negative. GTG-banding on prometaphase chromosomes showed an unbalanced translocation involving chr. 7. This was identified as an insertion of chr. 5 (q23.2q35.1) into distal 7q after FISH using chr. 5 and chr. 7 painting probes. The infant`s mother carries the balanced insertional rearrangement: 46,XX,dir ins(7,5)(q31.3;q23.2q35.1). This phenotype overlaps that of previously described duplications with the addition of giant cell hepatitis, coarsened facial features, gingival thickening, and flexion contractures, suggestive of a yet undiagnosed storage disorder.

  11. Refractory primary immune thrombocytopenia with subsequent del(5q) MDS: complete remission of both after lenalidomide.

    Mortensen, Thomas Bech; Frederiksen, Henrik; Marcher, Claus Werenberg; Preiss, Birgitte


    A patient with refractory primary immune thrombocytopenia (ITP) characterised by severe skin and mucosal bleedings was treated with several ITP-directed therapies including cyclophosphamide. He later developed therapy-related del(5q) myelodysplastic syndrome with no dysplastic morphological features in bone marrow. He remained severely thrombocytopenic, which suggests ongoing immune mediated platelet destruction. After two 3 week cycles of low-dose lenalidomide, complete cytogenetic remission and complete normalisation of platelet count were observed. This suggests that lenalidomide may be a viable treatment option for ITP in the presence of del(5q) not responding to standard treatments.

  12. Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDS.

    Krönke, Jan; Fink, Emma C; Hollenbach, Paul W; MacBeth, Kyle J; Hurst, Slater N; Udeshi, Namrata D; Chamberlain, Philip P; Mani, D R; Man, Hon Wah; Gandhi, Anita K; Svinkina, Tanya; Schneider, Rebekka K; McConkey, Marie; Järås, Marcus; Griffiths, Elizabeth; Wetzler, Meir; Bullinger, Lars; Cathers, Brian E; Carr, Steven A; Chopra, Rajesh; Ebert, Benjamin L


    Lenalidomide is a highly effective treatment for myelodysplastic syndrome (MDS) with deletion of chromosome 5q (del(5q)). Here, we demonstrate that lenalidomide induces the ubiquitination of casein kinase 1A1 (CK1α) by the E3 ubiquitin ligase CUL4-RBX1-DDB1-CRBN (known as CRL4(CRBN)), resulting in CK1α degradation. CK1α is encoded by a gene within the common deleted region for del(5q) MDS and haploinsufficient expression sensitizes cells to lenalidomide therapy, providing a mechanistic basis for the therapeutic window of lenalidomide in del(5q) MDS. We found that mouse cells are resistant to lenalidomide but that changing a single amino acid in mouse Crbn to the corresponding human residue enables lenalidomide-dependent degradation of CK1α. We further demonstrate that minor side chain modifications in thalidomide and a novel analogue, CC-122, can modulate the spectrum of substrates targeted by CRL4(CRBN). These findings have implications for the clinical activity of lenalidomide and related compounds, and demonstrate the therapeutic potential of novel modulators of E3 ubiquitin ligases.

  13. Duplication of 5q21 in a mildly retarded male and his non-retarded mother

    Stallard, R.; Zurcher, V.; Schwartz, S. [Case Western Reserve Univ., Cleveland, OH (United States)


    Euchromatic autosomal additions to chromosomal complements are typically associated with global effects including mental retardation (MR) and dysmorphism. We report a familial duplication that does not appear to cause consistent, significant effects. A hyperactive male with mild MR was referred for fra(X) testing at 8 yrs. His karyotype was fra(X) negative and normal except for an addition in one 5q. The abnormal 5 was also in the maternal karyotype, but all other parental chromosomes were normal. The addition (=8.5% the length of a 5) was interpreted as a duplication of band 5q21. FISH with Coatasome 5 (Oncor) showed the addition was from 5. The proband`s karyotype was designated 46,XY,dup(5)(q15q22.1)mat; his mother`s, 46,XX,dup(5)(q15q22.1). Single copy probes are being used to test the cytogenetic interpretation. At 39 yrs, the non-retarded, somewhat inattentive mother, who has a high school diploma and subsequent secretarial courses, cares for the proband and his chromosomally normal, but learning disabled sister at home. The family situation is chaotic with reported paternal psychiatric illness and abuse of the proband and his sister. The mother`s father is dead, but her four younger siblings and mother are reportedly normal. Their chromosomes have not been available. The proband was born at 40 weeks following an uneventful pregnancy, with length and weight at the 5-10th centiles. He walked and talked at about one year. At 9 yrs, his ht/wt ratio was 10th centile. Foot length as <3rd centile; soft masses were present on the anterior ankles. He was otherwise physically normal. His estimated I.Q. was 75 and he was severely hyperactive despite Ritalin. This is the first report of a familial duplication in 5q; no identical, isolated case is known. Although additional family members need evaluation, the presence of the dup(5q) in the non-retarded mother suggests that it may not be associated with the proband`s MR.

  14. Interstitial deletion of 5q33.3q35.1 in a boy with severe mental retardation

    Lee, Jin Hwan; Kim, Hyo Jeong; Yoon, Jung Min; Cheon, Eun Jung; Lim, Jae Woo; Ko, Kyong Og; Lee, Gyung Min


    Constitutional interstitial deletions of the long arm of chromosome 5 (5q) are quite rare, and the corresponding phenotype is not yet clearly delineated. Severe mental retardation has been described in most patients who present 5q deletions. Specifically, the interstitial deletion of chromosome 5q33.3q35.1, an extremely rare chromosomal aberration, is characterized by mental retardation, developmental delay, and facial dysmorphism. Although the severity of mental retardation varies across cas...

  15. [Lenalidomide treatment in myelodysplastic syndrome with 5q deletion--Czech MDS group experience].

    Jonášová, Anna; Červinek, Libor; Bělohlávková, Petra; Čermák, Jaroslav; Beličková, Monika; Rohoň, Petr; Černá, Olga; Hochová, Ivana; Šišková, Magda; Kačmářová, Karla; Janoušová, Eva


    Myelodysplastic syndrome (MDS) is a common hematological disease in patients over sixty. Despite intensive research, the therapy of this heterogeneous blood disease is complicated. In recent years, two new therapeutic approaches have been proposed: immunomodulation and demethylation therapy. Immunomodulation therapy with lenalidomide represents a meaningful advance in the treatment of anemic patients, specifically those with 5q- aberrations. As much as 60-70% of patients respond and achieve transfusion independence. We present the initial lenalidomide experience of the Czech MDS group. We analyze Czech MDS register data of 34 (31 female; 3 male; median age 69 years) chronically transfused low risk MDS patients with 5q- aberration treated by lenalidomide. Twenty-seven (79.4%) patients were diagnosed with 5q- syndrome, 5 patients with refractory anemia with multilineage dysplasia, 1 patient with refractory anemia with excess of blasts 1, and 1 patient with myelodysplastic/myeloproliferative unclassified. Response, as represented by achieving complete transfusion independence, was achieved in 91% of patients. A true 5q- syndrome diagnosis in most our patients may be responsible for such a high response rate. Complete cytogenetic response was reached in 15% of patients and partial cytogenetic response in 67%, within a median time of 12 months. TP53 mutation was detected in 15% (3 from 18 tested) and 2 of these patients progressed to higher grade MDS. The majority of patients tolerated lenalidomide very well. Based on this albeit small study, we present our findings of high lenalidomide efficacy as well as the basic principles and problems of lenalidomide therapy.

  16. Lenalidomide for myelodysplastic syndromes with del(5q): how long should it last?

    Vozella, Federico; Latagliata, Roberto; Carmosino, Ida; Volpicelli, Paola; Montagna, Chiara; Romano, Angela; Roberto, Amanda; Finsinger, Paola; Mancini, Marco; Breccia, Massimo; Oliva, Esther; Oliva, Esther


    Lenalidomide induces in patients with myelodysplastic syndrome (MDS) and del(5q) erythroid and cytogenetic response rates as high as 75% and 50%, respectively. It is still unclear, however, how long lenalidomide treatment should be continued and whether or not the drug could be interrupted. To assess the feasibility of lenalidomide discontinuation, we revised a cohort of 16 low-risk MDS patients with del(5q) treated at our institute in a phase II multicentric Italian study. Among the 12 responding patients, four discontinued lenalidomide while in complete response. All four patients needed during treatment a permanent lenalidomide reduction from 10 to 5 mg/day because of haematological toxicity (three patients) or grade 3 muscular and bone pain (one patient). At lenalidomide discontinuation after 16, 20, 27 and 20 months from the start, respectively, all four patients were in complete hematologic response and three forth in complete cytogenetic response. Three patients are still in response after 36, 30 and 20 months from lenalidomide discontinuation, respectively: The remaining patient relapsed after 20 months, and she is now receiving a new course of lenalidomide. In conclusion, long-lasting remissions are achievable in MDS patients with del(5q) in complete response after lenalidomide discontinuation.

  17. Radiation hybrid mapping of a cytokine gene cluster located in the proximal region of 5q

    Segal, A.L.; McPherson, J.D.; Wasmuth, J.J. [Univ. of California, Irvine (United States)


    The long (q) arm of chromosome 5 has been shown to contain a large number of genes encoding growth factors, growth factor receptors, hormone receptors and neurotransmitter receptors. IL-3, IL-4, IL-5, IL-9, IL-13, GM-CSF and IRF-1 are located in the 5q22-31.1 interval, while three GABA receptors map to 5q33-34. A number of receptors, including the prolactin and growth hormone receptors, the IL-7 receptor and the leukemia inhibitory factor receptor, map to proximal 5p. Genes encoding three of the complement components, C6, C7 and C9, are also located in the same region. YAC data indicates that C6 and C7 lie within 170 kb of each other. We have used a panel of 180 Chinese hamster-human radiation hybrids possessing fragments of human chromosome 5 to construct a physical map of this region of 5q. Two-point and multi-point analyses were done on the data and significant LOD scores (from 3 to 30) were observed. LIFR, PRLR, GHR, IL-7R, C6, C7, C9, TARS, and a number of CEPH-Genethon dinucleotide repeat markers were ordered and mapped. Yeast artificial chromosomes and cosmids have been isolated and inter-Alu PCR products from them are being used to construct a contig and to improve the physical map. The long term goal of this work is to identify and characterize new genes in the region.

  18. A locus regulating total serum IgE maps to chromosome 5q

    Amelung, P.J.; Panhuysen, C.I.M.; Postma, D.S. [Univ. of Maryland, Baltimore, MD (United States)]|[Beatrixoord Hospital (Netherlands)


    Familial aggregation of allergy has been demonstrated in numerous past studies. However, allergy is a complex disorder which is not inherited as a simple Mendelian trait. Total serum IgE levels correlate with the clinical expression of allergy and asthma and can be utilized as a quantitative measure of the allergic phenotype. We studied 92 families from Northern Holland ascertained through a parent with asthma who were originally studied between 1962-1970. Since there is a large number of candidate genes on chromosome 5q, families were genotyped for markers in this region. These genes either directly or indirectly regulate IgE production and the activation and proliferation of cellular elements that are involved in inflammation associated with allergy and asthma. They include IL-4, IL-3, IL-5, IL-9, IL-12, IL-13 and GM-CSF. Segregation analyses revealed recessive inheritance of `high` levels with a mean for the `low` phenotype of 1.51 (32 IU) and 2.52 (331 IU) for the `high` phenotype. Linkage of log IgE with markers on 5q was tested using the sib-pair and the LOD score methods with the genetic model obtained from the segregation analyses. These results provide evidence for a locus controlling IgE levels near the cytokine gene cluster on 5q. This region appears critical in susceptibility to allergy and asthma.

  19. Distal 5q trisomy resulting from an X;5 translocation detected by chromosome painting.

    Abuelo, D N; Ahsanuddin, A N; Mark, H F


    We describe the case of a 13-year-old girl with an apparently de novo unbalanced translocation resulting in the presence of additional chromosomal material on the short arm of one X chromosome, which was detected by conventional G-banding studies. Fluorescence in situ hybridization (FISH) using the Chromoprobe Multiprobe-M protocol confirmed that the additional chromosomal material originated from chromosome 5. The karyotype of this patient is now established to be 46,X,der(X) t(X;5)(p22.3;q33), with a deletion of Xp22.3-pter and partial trisomy of 5q33-qter. The distal 5q trisomy genotype has been associated with clinical signs that include growth and mental retardation, eczema, craniofacial anomalies, and malformations of heart, lungs, abdomen, limbs, and genitalia. Our patient also has short stature, a prominent nasal bridge, a flat philtrum, a thin upper lip, dental caries, and limb and cardiac malformations, but she appears to be mildly affected compared with previously reported cases. This is the first case of distal 5q trisomy arising from a translocation with the X chromosome. Replication studies on this patient show that the derivative t(X;5) chromosome is late replicating in almost all cells examined, which indicates that this chromosome is preferentially inactivated. However, the translocated segment of chromosome 5 appears to be early replicating, which implies that the trisomic 5q segment is transcriptionally active. We cannot determine from these studies whether all or only some genes in this segment are expressed, but this patient's relatively mild clinical signs suggest that the critical region(s) that contribute to the distal 5q trisomy phenotype are at least partly suppressed. A review of other patients with X-chromosome translocations indicates that many but not all of them also have attenuated phenotypes. The mechanism of inactivation of autosomal material attached to the X chromosome is complex, with varying effects on the phenotype of the

  20. Bilateral radial agenesis with absent thumbs, complex heart defect, short stature, and facial dysmorphism in a patient with pure distal microduplication of 5q35.2-5q35.3

    Jamsheer Aleksander


    Full Text Available Abstract Background A partial duplication of the distal long arm of chromosome 5 (5q35-- > qter is known to be associated with a distinct phenotype referred to as Hunter-McAlpine syndrome. Clinical spectrum of this disorder mainly consists of mental retardation, microcephaly, short stature, skeletal anomalies, and craniofacial dysmorphism featuring flat facies, micrognathia, large, low-set dysplastic ears, hypertelorism, almond-shaped, down-slanted palpebral fissures, epicanthal folds, small nose, long philtrum, small mouth, and thin upper lip. Less frequent remarkable findings include craniosynostosis, heart defect, hypoplastic phalanges, preaxial polydactyly, hypospadias, cryptorchidism, and inguinal hernia. In most patients with a partial duplication of 5q the aberration occurred due to an inherited unbalanced translocation, therefore the phenotype was not reflective of pure trisomy 5q. Case presentation We report on a 9.5-year-old boy with some feature of Hunter-McAlpine syndrome including short stature, complex heart defect (dextrocardia, dextroversion, PFO, bilateral cryptorchidism, hypothyroidism, and craniofacial dysmorphism. Additionally, bilateral radial agenesis with complete absence of Ist digital rays, ulnar hypoplasia with bowing, choroidal and retinal coloboma, abnormal biliary vesicle were identified, which have never been noted in 5q trisomy patients. Karyotype analysis, sequencing and MLPA for TBX5 and SALL4 genes were unremarkable. Array comparative genomic hybridization detected a duplication on 5q35.2-5q35.3, resulting from a de novo chromosomal rearrangement. Our proband carried the smallest of all previously reported pure distal 5q trisomies encompassing terminal 5.4-5.6 Mb and presented with the most severe limb malformation attributed to the increased number of distal 5q copies. Conclusions We postulate that a terminal distal trisomy of 5q35.2-5q35.3, which maps 1.1 Mb telomeric to the MSX2 gene is causative for both

  1. Interstitial deletion of 5q33.3q35.1 in a boy with severe mental retardation.

    Lee, Jin Hwan; Kim, Hyo Jeong; Yoon, Jung Min; Cheon, Eun Jung; Lim, Jae Woo; Ko, Kyong Og; Lee, Gyung Min


    Constitutional interstitial deletions of the long arm of chromosome 5 (5q) are quite rare, and the corresponding phenotype is not yet clearly delineated. Severe mental retardation has been described in most patients who present 5q deletions. Specifically, the interstitial deletion of chromosome 5q33.3q35.1, an extremely rare chromosomal aberration, is characterized by mental retardation, developmental delay, and facial dysmorphism. Although the severity of mental retardation varies across cases, it is the most common feature described in patients who present the 5q33.3q35.1 deletion. Here, we report a case of a de novo deletion of 5q33.3q35.1, 46,XY,del(5)(q33.3q35.1) in an 11-year-old boy with mental retardation; to the best of our knowledge this is the first case in Korea to be reported. He was diagnosed with severe mental retardation, developmental delay, facial dysmorphisms, dental anomalies, and epilepsy. Chromosomal microarray analysis using the comparative genomic hybridization array method revealed a 16-Mb-long deletion of 5q33. 3q35.1(156,409,412-172,584,708)x1. Understanding this deletion may help draw a rough phenotypic map of 5q and correlate the phenotypes with specific chromosomal regions. The 5q33.3q35.1 deletion is a rare condition; however, accurate diagnosis of the associated mental retardation is important to ensure proper genetic counseling and to guide patients as part of long-term management.

  2. Clinical phenotype and candidate genes for the 5q31.3 microdeletion syndrome.

    Hosoki, Kana; Ohta, Tohru; Natsume, Jun; Imai, Sumiko; Okumura, Akihisa; Matsui, Takeshi; Harada, Naoki; Bacino, Carlos A; Scaglia, Fernando; Jones, Jeremy Y; Niikawa, Norio; Saitoh, Shinji


    Array-based technologies have led to the identification of many novel microdeletion and microduplication syndromes demonstrating multiple congenital anomalies and intellectual disability (MCA/ID). We have used chromosomal microarray analysis for the evaluation of patients with MCA/ID and/or neonatal hypotonia. Three overlapping de novo microdeletions at 5q31.3 with the shortest region of overlap (SRO) of 370 kb were detected in three unrelated patients. These patients showed similar clinical features including severe neonatal hypotonia, neonatal feeding difficulties, respiratory distress, characteristic facial features, and severe developmental delay. These features are consistent with the 5q31.3 microdeletion syndrome originally proposed by Shimojima et al., providing further evidence that this syndrome is clinically discernible. The 370 kb SRO encompasses only four RefSeq genes including neuregulin 2 (NRG2) and purine-rich element binding protein A (PURA). NRG2 is one of the members of the neuregulin family related to neuronal and glial cell growth and differentiation, thus making NRG2 a good candidate for the observed phenotype. Moreover, PURA is also a good candidate because Pura-deficient mice demonstrate postnatal neurological manifestations.

  3. The gene for human glutaredoxin (GLRX) is localized to human chromosome 5q14

    Padilla, C.A.; Holmgren, A. [Karolinska Inst., Stockholm (Sweden); Bajalica, S.; Lagercrantz, J. [Karolinska Hospital, Stockholm (Sweden)


    Glutaredoxin is a small protein (12 kDa) catalyzing glutathione-dependent disulfide oxidoreduction reactions in a coupled system with NADPH, GSH, and glutathione reductase. A cDNA encoding the human glutaredoxin gene (HGMW-approved symbol GLRX) has recently been isolated and cloned from a human fetal spleen cDNA library. The screening of a human fetal spleen cDNA library. The screening of a human genomic library in Charon 4A led to the identification of three genomic clones. Using fluorescence in situ hybridization to metaphase chromosomes with one genomic clone as a probe, the human glutaredoxin gene was localized to chromosomal region 5q14. This localization at chromosome 5 was in agreement with the somatic cell hybrid analysis, using DNA from a human-hamster and a human-mouse hybrid panel and using a human glutaredoxin cDNA as a probe. 13 refs., 2 figs.

  4. A YAC contig of approximately 3 Mb from human chromosome 5q31 [yields] q33

    Li, Xiang; Wang Jabs, E.; Hawkins, A.L.; Griffin, C.A. (John Hopkins School of Medicine, Baltimore, MD (United States)); Wise, C.A.; Lovett, M. (Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)); Le Paslier, D. (CEPH, Paris (France)); Pittler, S.J. (Univ. of South Alabama College of Medicine, Mobile, AL (United States))


    The human chromosome 5q31-q33 region contains an interesting cluster of growth factor and receptor genes. In addition, several genetic disease loci have been localized within this region, but have not as yet been isolated as molecular clones. These include those loci involved in autosomal dominant limb-girdle muscular dystrophy, diastrophic dysplasia, Treacher Collins syndrome, and myeloid disorders associated with the 5q-syndrome. A yeast artificial chromosome (YAC) contig of this region would assist in the further localization and isolation of these genes. The authors have used YACs isolated from the Washington University and Centre d'Etude du Polymorphisme Humain YAC libraries, including YACs from the large insert (mega) YAC library to build a contig greater than 3 Mb in size. An STS content strategy coupled with limited walking from YAC ends was used to isolate 22 overlapping YACs with as much as sixfold coverage. A total of 20 STSs, derived from genes, anonymous sequences, and vector Alu-PCR or inverse PCR products, were used to compile this contig. The order of loci, centromere-GRL-D5S207-D5S70-D5S545-D5S546-D5S547-D5S68-D5S548-D5S210-D5S549-D5S686- ADRB2-D5S559-CSF1R-D5S551-RPS14-D5S519-SPARC-telomere, was derived from the overlapping clones. This contig and clones derived from it will be useful substrates in selecting candidate cDNAs for the disease loci in this interval. 45 refs., 1 fig., 2 tabs.

  5. Variation in conserved non-coding sequences on chromosome 5q andsusceptibility to asthma and atopy

    Donfack, Joseph; Schneider, Daniel H.; Tan, Zheng; Kurz,Thorsten; Dubchak, Inna; Frazer, Kelly A.; Ober, Carole


    Background: Evolutionarily conserved sequences likely havebiological function. Methods: To determine whether variation in conservedsequences in non-coding DNA contributes to risk for human disease, westudied six conserved non-coding elements in the Th2 cytokine cluster onhuman chromosome 5q31 in a large Hutterite pedigree and in samples ofoutbred European American and African American asthma cases and controls.Results: Among six conserved non-coding elements (>100 bp,>70percent identity; human-mouse comparison), we identified one singlenucleotide polymorphism (SNP) in each of two conserved elements and sixSNPs in the flanking regions of three conserved elements. We genotypedour samples for four of these SNPs and an additional three SNPs each inthe IL13 and IL4 genes. While there was only modest evidence forassociation with single SNPs in the Hutterite and European Americansamples (P<0.05), there were highly significant associations inEuropean Americans between asthma and haplotypes comprised of SNPs in theIL4 gene (P<0.001), including a SNP in a conserved non-codingelement. Furthermore, variation in the IL13 gene was strongly associatedwith total IgE (P = 0.00022) and allergic sensitization to mold allergens(P = 0.00076) in the Hutterites, and more modestly associated withsensitization to molds in the European Americans and African Americans (P<0.01). Conclusion: These results indicate that there is overalllittle variation in the conserved non-coding elements on 5q31, butvariation in IL4 and IL13, including possibly one SNP in a conservedelement, influence asthma and atopic phenotypes in diversepopulations.

  6. Targeted re-sequencing analysis of 25 genes commonly mutated in myeloid disorders in del(5q) myelodysplastic syndromes

    Fernandez-Mercado, Marta; Burns, Adam; Pellagatti, Andrea; Giagounidis, Aristoteles; Germing, Ulrich; Agirre, Xabier; Prosper, Felipe; Aul, Carlo; Killick, Sally; Wainscoat, James S.; Schuh, Anna; Boultwood, Jacqueline


    Interstitial deletion of chromosome 5q is the most common chromosomal abnormality in myelodysplastic syndromes. The catalogue of genes involved in the molecular pathogenesis of myelodysplastic syndromes is rapidly expanding and next-generation sequencing technology allows detection of these mutations at great depth. Here we describe the design, validation and application of a targeted next-generation sequencing approach to simultaneously screen 25 genes mutated in myeloid malignancies. We used this method alongside single nucleotide polymorphism-array technology to characterize the mutational and cytogenetic profile of 43 cases of early or advanced del(5q) myelodysplastic syndromes. A total of 29 mutations were detected in our cohort. Overall, 45% of early and 66.7% of advanced cases had at least one mutation. Genes with the highest mutation frequency among advanced cases were TP53 and ASXL1 (25% of patients each). These showed a lower mutation frequency in cases of 5q- syndrome (4.5% and 13.6%, respectively), suggesting a role in disease progression in del(5q) myelodysplastic syndromes. Fifty-two percent of mutations identified were in genes involved in epigenetic regulation (ASXL1, TET2, DNMT3A and JAK2). Six mutations had allele frequencies <20%, likely below the detection limit of traditional sequencing methods. Genomic array data showed that cases of advanced del(5q) myelodysplastic syndrome had a complex background of cytogenetic aberrations, often encompassing genes involved in myeloid disorders. Our study is the first to investigate the molecular pathogenesis of early and advanced del(5q) myelodysplastic syndromes using next-generation sequencing technology on a large panel of genes frequently mutated in myeloid malignancies, further illuminating the molecular landscape of del(5q) myelodysplastic syndromes. PMID:23831921

  7. Discovery of Genetic Variation on Chromosome 5q22 Associated with Mortality in Heart Failure.

    J Gustav Smith


    Full Text Available Failure of the human heart to maintain sufficient output of blood for the demands of the body, heart failure, is a common condition with high mortality even with modern therapeutic alternatives. To identify molecular determinants of mortality in patients with new-onset heart failure, we performed a meta-analysis of genome-wide association studies and follow-up genotyping in independent populations. We identified and replicated an association for a genetic variant on chromosome 5q22 with 36% increased risk of death in subjects with heart failure (rs9885413, P = 2.7x10-9. We provide evidence from reporter gene assays, computational predictions and epigenomic marks that this polymorphism increases activity of an enhancer region active in multiple human tissues. The polymorphism was further reproducibly associated with a DNA methylation signature in whole blood (P = 4.5x10-40 that also associated with allergic sensitization and expression in blood of the cytokine TSLP (P = 1.1x10-4. Knockdown of the transcription factor predicted to bind the enhancer region (NHLH1 in a human cell line (HEK293 expressing NHLH1 resulted in lower TSLP expression. In addition, we observed evidence of recent positive selection acting on the risk allele in populations of African descent. Our findings provide novel genetic leads to factors that influence mortality in patients with heart failure.

  8. Faithful expression of the human 5q31 cytokine cluster intransgenic mice

    Lacy, Dee A.; Wang, Zhi-En; Symula, Derek J.; McArthur, CliffordJ.; Rubin, Edward M.; Frazer, Kelly A.; Locksley, Richard M.


    ILs 4,5, and 13, cardinal cytokines produced by Th2 cells,are coordinately expressed and clustered in the 150-kb syntenic regions on mouse chromosome 11 and human chromosome 5q31. We analyzed two sets of human yeast artificial chromosome transgenic mice that contained the5931cytokines to assess whether conserved sequences required for their coordinate and cell-specific regulation are contained within the cytokine cluster itself. Human Il-4, IL-13, and Il-5 were expressed under Th2, but not Th1, conditions in vitro. Each of these cytokines was produced during infection with Nippostrongylus brasiliensis, a Th2 inducing stimulus, and human Il-4 was generated after activation of NK T cells in vivo.Consistently fewer cells produced the endogenous mouse cytokines in transgenic than in control mice, suggesting competition for stable expression between the mouse and human genes. These data imply the existence of both conserved trans-activating factors and cis-regulatory elements that underlie the coordinate expression and lineage specificity of the type 2 ctyokine genes in lymphocytes.

  9. A Patient with Interstitial 5q21 Deletion, Familial Adenomatous Polyposis, Dysmorphic Features, and Profound Neurologic Dysfunction

    Manoochehr Karjoo


    Full Text Available Familial adenomatous polyposis (FAP is a hereditary autosomal dominant cancer syndrome, results from germ line mutation or deletion of the Adenomatous Polyposis Coli (APC gene on chromosome 5q21. Patients with FAP suffer from multiple polyps mainly at the colorectal region as well as other parts of the gastrointestinal tract, which has propensity to transform into carcinoma. FAP has also been well described in association with various syndromic extra-gastrointestinal manifestations. Less commonly, patients with FAP present with varying degrees of cognitive dysfunction and developmental delay, though the reason for the association is unclear. Herein, we report the case of a male patient born with an interstitial deletion of chromosome 5q, 46,XY, del(5 (q14q23, presenting with familial adenomatous polyposis (FAP, profound developmental delay, cognitive dysfunction, and multiple congenital anomalies including talipes equinovarus, agenesis of the corpus callosum, and dysmorphic facial features.

  10. New superhindered polydentate polyphosphine ligands P(CH2CH2P(t)Bu2)3, PhP(CH2CH2P(t)Bu2)2, P(CH2CH2CH2P(t)Bu2)3, and their ruthenium(II) chloride complexes.

    Gilbert-Wilson, Ryan; Field, Leslie D; Bhadbhade, Mohan M


    The synthesis and characterization of the extremely hindered phosphine ligands, P(CH(2)CH(2)P(t)Bu(2))(3) (P(2)P(3)(tBu), 1), PhP(CH(2)CH(2)P(t)Bu(2))(2) (PhP(2)P(2)(tBu), 2), and P(CH(2)CH(2)CH(2)P(t)Bu(2))(3) (P(3)P(3)(tBu), 3) are reported, along with the synthesis and characterization of ruthenium chloro complexes RuCl(2)(P(2)P(3)(tBu)) (4), RuCl(2)(PhP(2)P(2)(tBu)) (5), and RuCl(2)(P(3)P(3)(tBu)) (6). The bulky P(2)P(3)(tBu) (1) and P(3)P(3)(tBu) (3) ligands are the most sterically encumbered PP(3)-type ligands so far synthesized, and in all cases, only three phosphorus donors are able to bind to the metal center. Complexes RuCl(2)(PhP(2)P(2)(tBu)) (5) and RuCl(2)(P(3)P(3)(tBu)) (6) were characterized by crystallography. Low temperature solution and solid state (31)P{(1)H} NMR were used to demonstrate that the structure of RuCl(2)(P(2)P(3)(tBu)) (4) is probably analogous to that of RuCl(2)(PhP(2)P(2)(tBu)) (5) which had been structurally characterized.

  11. A Locus at 5q33.3 Confers Resistance to Tuberculosis in Highly Susceptible Individuals

    Sobota, Rafal S.; Stein, Catherine M.; Kodaman, Nuri; Scheinfeldt, Laura B.; Maro, Isaac; Wieland-Alter, Wendy; Igo, Robert P.; Magohe, Albert; Malone, LaShaunda L.; Chervenak, Keith; Hall, Noemi B.; Modongo, Chawangwa; Zetola, Nicola; Matee, Mecky; Joloba, Moses; Froment, Alain; Nyambo, Thomas B.; Moore, Jason H.; Scott, William K.; Lahey, Timothy; Boom, W. Henry; von Reyn, C. Fordham; Tishkoff, Sarah A.; Sirugo, Giorgio; Williams, Scott M.


    Immunosuppression resulting from HIV infection increases the risk of progression to active tuberculosis (TB) both in individuals newly exposed to Mycobacterium tuberculosis (MTB) and in those with latent infections. We hypothesized that HIV-positive individuals who do not develop TB, despite living in areas where it is hyperendemic, provide a model of natural resistance. We performed a genome-wide association study of TB resistance by using 581 HIV-positive Ugandans and Tanzanians enrolled in prospective cohort studies of TB; 267 of these individuals developed active TB, and 314 did not. A common variant, rs4921437 at 5q33.3, was significantly associated with TB (odds ratio = 0.37, p = 2.11 × 10−8). This variant lies within a genomic region that includes IL12B and is embedded in an H3K27Ac histone mark. The locus also displays consistent patterns of linkage disequilibrium across African populations and has signals of strong selection in populations from equatorial Africa. Along with prior studies demonstrating that therapy with IL-12 (the cytokine encoded in part by IL12B, associated with longer survival following MTB infection in mice deficient in CD4 T cells), our results suggest that this pathway might be an excellent target for the development of new modalities for treating TB, especially for HIV-positive individuals. Our results also indicate that studying extreme disease resistance in the face of extensive exposure can increase the power to detect associations in complex infectious disease. PMID:26942285

  12. The association between DNA copy number aberrations at chromosome 5q22 and gastric cancer.

    Pei-Chien Tsai

    Full Text Available BACKGROUND: Gastric cancer is common cancer. Discovering novel genetic biomarkers might help to identify high-risk individuals. Copy number variation (CNV has recently been shown to influence risk for several cancers. The aim of the present study was sought to test the association between copy number at a variant region and GC. METHODS: A total of 110 gastric cancer patients and 325 healthy volunteers were enrolled in this study. We searched for a CNV and found a CNV (Variation 7468 containing part of the APC gene, the SRP19 gene and the REEP5 gene. We chose four probes targeting at APC-intron8, APC-exon9, SRP19 and REEP5 to interrogate this CNV. Specific Taqman probes labeled by different reporter fluorophores were used in a real-time PCR platform to obtain copy number. Both the original non-integer data and transformed integer data on copy number were used for analyses. RESULTS: Gastric caner patients had a lower non-integer copy number than controls for the APC-exon9 probe (Adjusted p = 0.026 and SRP19 probe (Adjusted p = 0.002. The analysis of integer copy number yielded a similar pattern although less significant (Adjusted p = 0.07 for APC-exon9 probe and Adjusted p = 0.02 for SRP19 probe. CONCLUSIONS: Losses of a CNV at 5q22, especially in the DNA region surrounding APC-exon 9, may be associated with a higher risk of gastric cancer.

  13. Analysis of the efficacy of lenalidomide in patients with intermediate-1 risk myelodysplastic syndrome without 5q deletion.

    Yang, Yan; Gao, Sujun; Fan, Hongqiong; Lin, Hai; Li, Wei; Wang, Juan


    The aim of this study was to evaluate the efficacy and adverse effects of lenalidomide in the treatment of intermediate-1 risk non-5q deletion [non-del (5q)] myelodysplastic syndrome (MDS). A total of 30 patients with MDS were classified through G-banding chromosome karyotype analysis and fluorescence in situ hybridization (FISH). According to the International Prognostic Scoring System scores, among the 30 patients, 23 and seven cases had scores of 0.5 and 1.0, respectively. Lenalidomide (Revlimid(®)), 10 mg/day) was administered for 21 days every 28 days. All 30 cases were treated with lenalidomide for at least three cycles, including 20 cases with four cycles. The patients did not require erythropoietin, cyclosporine or iron chelation treatments. Statistical analysis was performed using SPSS statistical software version 13.0, and comparisons among groups were conducted using a t-test. The efficacy of lenalidomide was demonstrated in patients with intermediate-1 risk non-del (5q) MDS. Peripheral blood cell counts were improved following treatment, and absolute neutrophil, haemoglobin and platelet counts increased following 2-4 cycles of treatment. All patients became stable having undergone three cycles of treatment; however, 17 patients with chromosomal abnormalities had no cytogenetic response to the treatment, as confirmed through the FISH test. Patients with intermediate-1 risk non-del (5q) MDS treated with lenalidomide did not achieve complete haematological remission, although they demonstrated haematological improvement.

  14. p53 protein expression independently predicts outcome in patients with lower-risk myelodysplastic syndromes with del(5q)

    Saft, L.; Karimi, M.; Ghaderi, M.; Matolcsy, A.; Mufti, G.J.; Kulasekararaj, A.; Gohring, G.; Giagounidis, A.; Selleslag, D.; Muus, P.; Sanz, G.; Mittelman, M.; Bowen, D.; Porwit, A.; Fu, T.; Backstrom, J.; Fenaux, P.; MacBeth, K.J.; Hellstrom-Lindberg, E.


    Del(5q) myelodysplastic syndromes defined by the International Prognostic Scoring System as low- or intermediate-1-risk (lower-risk) are considered to have an indolent course; however, recent data have identified a subgroup of these patients with more aggressive disease and poorer outcomes. Using de

  15. Cytogenetic and molecular predictors of response in patients with myeloid malignancies without del[5q] treated with lenalidomide

    Sugimoto Yuka


    Full Text Available Abstract Background While lenalidomide (LEN shows high efficacy in myelodysplastic syndromes (MDS with del[5q], responses can be also seen in patients presenting without del[5q]. We hypothesized that improved detection of chromosomal abnormalities with new karyotyping tools may better predict response to LEN. Design and methods We have studied clinical, molecular and cytogenetic features of 42 patients with MDS, myeloproliferative neoplasms (MPN, MDS/MPN overlap syndromes and secondary acute myeloid leukemia (sAML without del[5q] by metaphase cytogenetics (MC who underwent therapy with LEN. Results Fluorescence in situ hybridization (FISH or single nucleotide polymorphism array (SNP-A-based karyotyping marginally increased the diagnostic yield over MC, detecting 2/42 (4.8% additional cases with del[5q], one of whom were responded to LEN. Responses were more often observed in patients with a normal karyotype by MC (60% vs abnormal MC; 17%, p = .08 and those with gain of chromosome 8 material by either of all 3 karyotyping methods (83% vs all other chromosomal abnormalities; 44% p = .11. However, 5 out of those 6 patients received combined LEN/AZA therapy and it may also suggest those with gain of chromosome 8 material respond well to AZA. The addition of FISH or SNP-A did not improve the predictive value of normal cytogenetics by MC. Mutational analysis of TET2, UTX, CBL, EZH2, ASXL1, TP53, RAS, IDH1/2, and DNMT-3A was performed on 21 of 41 patients, and revealed 13 mutations in 11 patients, but did not show any molecular markers of responsiveness to LEN. Conclusions Normal karyotype and gain of chromosome 8 material was predictive of response to LEN in non-del[5q] patients with myeloid malignancies.

  16. A novel 5q11.2 microdeletion in a child with mild developmental delay and dysmorphic features.

    Fontana, Paolo; Tortora, Cristina; Petillo, Roberta; Falco, Mariateresa; Miniero, Martina; De Brasi, Davide; Pisanti, Maria Antonietta


    5q11.2 Deletion is a very rare genomic disorder, and its clinical phenotype has not yet been characterized. This report describes a patient with an 8.6 Mb deletion, showing hypotonia, mild developmental delay, short stature, and distinctive dysmorphic features (frontal bossing, square face, deep-set eyes, prominent columella, long philtrum, thin lips). © 2016 Wiley Periodicals, Inc.

  17. A syndromic form of Pierre Robin sequence is caused by 5q23 deletions encompassing FBN2 and PHAX.

    Ansari, Morad; Rainger, Jacqueline K; Murray, Jennie E; Hanson, Isabel; Firth, Helen V; Mehendale, Felicity; Amiel, Jeanne; Gordon, Christopher T; Percesepe, Antonio; Mazzanti, Laura; Fryer, Alan; Ferrari, Paola; Devriendt, Koenraad; Temple, I Karen; FitzPatrick, David R


    Pierre Robin sequence (PRS) is an aetiologically distinct subgroup of cleft palate. We aimed to define the critical genomic interval from five different 5q22-5q31 deletions associated with PRS or PRS-associated features and assess each gene within the region as a candidate for the PRS component of the phenotype. Clinical array-based comparative genome hybridisation (aCGH) data were used to define a 2.08 Mb minimum region of overlap among four de novo deletions and one mother-son inherited deletion associated with at least one component of PRS. Commonly associated anomalies were talipes equinovarus (TEV), finger contractures and crumpled ear helices. Expression analysis of the orthologous genes within the PRS critical region in embryonic mice showed that the strongest candidate genes were FBN2 and PHAX. Targeted aCGH of the critical region and sequencing of these genes in a cohort of 25 PRS patients revealed no plausible disease-causing mutations. In conclusion, deletion of ∼2 Mb on 5q23 region causes a clinically recognisable subtype of PRS. Haploinsufficiency for FBN2 accounts for the digital and auricular features. A possible critical region for TEV is distinct and telomeric to the PRS region. The molecular basis of PRS in these cases remains undetermined but haploinsufficiency for PHAX is a plausible mechanism.

  18. Mass-dependent dynamics of the luminescent exchange reactions C+(2P), P+(3P) + H2, D2, HD

    Glenewinkel-Meyer, Th; Hoppe, U.; Kowalski, A.; Ottinger, Ch; Rabenda, D.


    Chemiluminescent ion/molecule reactions of ground state C+ and P+ ions with H2, D2 and HD have been studied in an ion beam/target gas cell arrangement. Emission spectra of CH+, CD+ (A 1II) and of PH+, PD+ (A 2[Delta]) were observed with up to 1 Å FWHM resolution and at collision energies from threshold ([approximate] 3 eV) to 8 eVc.m. (centre-of-mass) and 15eVc.m., respectively. Very detailed computer simulations of the spectral contours were done, including ab initio transition moments and, in the case of PH+/PD+, the effects of predissociation. In simulating the spectra obtained with HD, the overlapped hydride and deuteride product ion spectra could be isolated by varying the respective weighting factors to achieve an optimum overall fit. In the case of C+ + HD, the two components were found to have very similar rovibrational distributions as with the products from C+ + H2 and C+ + D2. In the P+ case, however, the rotational, although not the vibrational, distributions were found to be significantly different for the isotopically mixed and the pure reactions. The cross-sections showed an intermolecular isotope effect only for C+ + H2 vs. C+ + D2 at high energies. However, both with C+ + HD and P+ + HD, a very strong intramolecular isotope effect, i.e. an energy-dependent branching ratio, was observed: at low energies deuteride formation prevails, at high energies hydride. This behaviour is discussed in terms of an impulsive collision model, assuming the "pairwise" relative kinetic energy between the reacting atoms to be the determining factor. On the basis of the measured cross-section curves for the H2 and D2 reactions, the energy-dependent hydride/deuteride ratio in the HD reaction can then be predicted. The agreement with the experimental results is excellent in the P+ case, but only moderate for the C+ reactions. Even the P+ reaction, however, does not occur via the spectator stripping mechanisms. The spectra show an energy-independent vibrational excitation, contrary to the stripping model assumption. The impulsive model was also extended to an analysis of the relative mean product angular momenta. Again it performs well for P+ + H2, D2, HD, but cannot fit the C+ + HD case.

  19. Thermoelastic properties of Zn3P2

    Gerward, Leif; Olsen, J. Staun; Waśkowska, A.


    The bulk modulus and thermal expansion of Zn3P2 has been investigated at pressures up to 21GPa and temperatures down to 100K. The experimental zero-pressure bulk modulus is 80.7 ± 1.8GPa, in accordance with the bulk modulus scaling and lattice properties of the related compound Cd3P2. A tetragonal...... to orthorhombic phase transformation occurs above 11GPa with a relative volume change of-7.1%. Values for the thermal expansion coefficient are reported at 293, 200 and 100K....

  20. Definition of 5q11.2 Microdeletion Syndrome Reveals Overlap with CHARGE Syndrome and 22q11 Deletion Syndrome Phenotypes

    Blok, Charlotte Snijders; Corsten-Janssen, Nicole; FitzPatrick, David R.; Romano, Corrado; Fichera, Marco; Vitello, Girolamo Aurelio; Willemsen, Marjolein H.; Schoots, Jeroen; Pfundt, Rolph; van Ravenswaaij-Arts, Conny M. A.; Hoefsloot, Lies; Kleefstra, Tjitske


    Microdeletions of the 5q11.2 region are rare; in literature only two patients with a deletion in this region have been reported so far. In this study, we describe four additional patients and further define this new 5q11.2 microdeletion syndrome. A comparison of the features observed in all six pati

  1. Fine-scale mapping of the 5q11.2 breast cancer locus reveals at least three independent risk variants regulating MAP3K1

    D.M. Glubb (Dylan); M. Maranian (Melanie); K. Michailidou (Kyriaki); K.A. Pooley (Karen); K.B. Meyer (Kerstin); S. Kar (Siddhartha); A.F.C. Carlebur; M. O'Reilly (Marian); J.A. Betts (Joshua); K.M. Hillman (Kristine); S. Kaufmann (Susanne); J. Beesley (Jonathan); S. Canisius (Sander); J.L. Hopper (John); M.C. Southey (Melissa); H. Tsimiklis (Helen); C. Apicella (Carmel); M.K. Schmidt (Marjanka); A. Broeks (Annegien); F.B.L. Hogervorst (Frans); C.E. van der Schoot (Ellen); K.R. Muir (K.); A. Lophatananon (Artitaya); S. Stewart-Brown (Sarah); P. Siriwanarangsan (Pornthep); P.A. Fasching (Peter); M. Ruebner (Matthias); A.B. Ekici (Arif); M.W. Beckmann (Matthias W.); J. Peto (Julian); I. dos Santos Silva (Isabel); O. Fletcher (Olivia); N. Johnson (Nichola); P.D.P. Pharoah (Paul D.P.); M.K. Bolla (Manjeet); Q. Wang (Qing); J. Dennis (Joe); E.J. Sawyer (Elinor); I.P. Tomlinson (Ian); M. Kerin (Michael); N. Miller (Nicola); B. Burwinkel (Barbara); F. Marme (Federick); R. Yang (Rongxi); H. Surowy (Harald); P. Guénel (Pascal); T. Truong (Thérèse); F. Menegaux (Florence); M. Sanchez (Marie); S.E. Bojesen (Stig); B.G. Nordestgaard (Børge); S.F. Nielsen (Sune); H. Flyger (Henrik); A. González-Neira (Anna); J. Benítez (Javier); M.P. Zamora (Pilar); J.I. Arias Pérez (José Ignacio); H. Anton-Culver (Hoda); S.L. Neuhausen (Susan); H. Brenner (Hermann); A.K. Dieffenbach (Aida Karina); V. Arndt (Volker); C. Stegmaier (Christa); A. Meindl (Alfons); R.K. Schmutzler (Rita); H. Brauch (Hiltrud); Y.-D. Ko (Yon-Dschun); T. Brüning (Thomas); H. Nevanlinna (Heli); T.A. Muranen (Taru); K. Aittomäki (Kristiina); C. Blomqvist (Carl); K. Matsuo (Keitaro); H. Ito (Hidemi); H. Iwata (Hisato); H. Tanaka (Hideo); T. Dörk (Thilo); N.V. Bogdanova (Natalia); S. Helbig (Sonja); A. Lindblom (Annika); S. Margolin (Sara); A. Mannermaa (Arto); V. Kataja (Vesa); V-M. Kosma (Veli-Matti); J.M. Hartikainen (J.); A.H. Wu (Anna H.); C.-C. Tseng (Chiu-Chen); D. Van Den Berg (David); D.O. Stram (Daniel O.); D. Lambrechts (Diether); H. Zhao (Hui); C. Weltens (Caroline); E. van Limbergen (Erik); J. Chang-Claude (Jenny); D. Flesch-Janys (Dieter); A. Rudolph (Anja); P. Seibold (Petra); P. Radice (Paolo); P. Peterlongo (Paolo); M. Barile (Monica); F. Capra (Fabio); F.J. Couch (Fergus); J.E. Olson (Janet); B. Hallberg (Boubou); C. Vachon (Celine); G.G. Giles (Graham); R.L. Milne (Roger); C.A. McLean (Catriona Ann); C.A. Haiman (Christopher); B.E. Henderson (Brian); F.R. Schumacher (Fredrick); L. Le Marchand (Loic); J. Simard (Jacques); M.S. Goldberg (Mark); F. Labrèche (France); M. Dumont (Martine); S.-H. Teo; C.H. Yip (Cheng Har); M.-H. See (Mee-Hoong); B.K. Cornes (Belinda); C.-Y. Cheng (Ching-Yu); M.K. Ikram (Kamran); V. Kristensen (Vessela); W. Zheng (Wei); S.L. Halverson (Sandra L.); M. Shrubsole (Martha); J. Long (Jirong); R. Winqvist (Robert); K. Pykäs (Katri); A. Jukkola-Vuorinen (Arja); S. Kauppila (Saila); I.L. Andrulis (Irene); J.A. Knight (Julia); G. Glendon (Gord); S. Tchatchou (Sandrine); P. Devilee (Peter); R.A.E.M. Tollenaar (Rob); C.M. Seynaeve (Caroline); C.J. van Asperen (Christi); M. García-Closas (Montserrat); J.D. Figueroa (Jonine); S.J. Chanock (Stephen); J. Lissowska (Jolanta); K. Czene (Kamila); D. Klevebring (Daniel); H. Darabi (Hatef); M. Eriksson (Mikael); M.J. Hooning (Maartje); A. Hollestelle (Antoinette); J.W.M. Martens (John W. M.); J.M. Collee (Margriet); P. Hall (Per); J. Li (Jingmei); K. Humphreys (Keith); X.-O. Shu (Xiao-Ou); W. Lu (Wei); Y.-T. Gao (Yu-Tang); H. Cai (Hui); A. Cox (Angela); S.S. Cross (Simon); M.W.R. Reed (Malcolm); W.J. Blot (William); L.B. Signorello (Lisa B.); Q. Cai (Qiuyin); M. Shah (Mitul); M. Ghoussaini (Maya); D. Kang (Daehee); J.-Y. Choi (J.); S.K. Park (Sue); D-Y. Noh (Dong-Young); M. Hartman (Mikael); X. Miao; W.-Y. Lim (Wei-Yen); A. Tang (Anthony); U. Hamann (Ute); D. Torres (Diana); A. Jakubowska (Anna); J. Lubinski (Jan); K. Jaworska (Katarzyna); K. Durda (Katarzyna); S. Sangrajrang (Suleeporn); V. Gaborieau (Valerie); P. Brennan (Paul); J.D. McKay (James); C. Olswold (Curtis); S. Slager (Susan); A.E. Toland (Amanda); D. Yannoukakos (Drakoulis); C-Y. Shen (Chen-Yang); P.-E. Wu (Pei-Ei); J-C. Yu (Jyh-Cherng); M.-F. Hou (Ming-Feng); A.J. Swerdlow (Anthony ); A. Ashworth (Alan); N. Orr (Nick); M. Jones (Michael); G. Pita (G.); M.R. Alonso (M Rosario); N. Álvarez (Nuria); D. Herrero (Daniel); D.C. Tessier (Daniel C.); D. Vincent (Daniel); F. Bacot (Francois); C. Luccarini (Craig); C. Baynes (Caroline); S. Ahmed (Shahana); S. Healey (Sue); M. Brown (Melissa); B.A.J. Ponder (Bruce A.J.); G. Chenevix-Trench (Georgia); D. Thompson (Deborah); S.L. Edwards (Stacey); D.F. Easton (Douglas); A.M. Dunning (Alison); J.D. French (Juliet)


    textabstractGenome-wide association studies (GWASs) have revealed SNP rs889312 on 5q11.2 to be associated with breast cancer risk in women of European ancestry. In an attempt to identify the biologically relevant variants, we analyzed 909 genetic variants across 5q11.2 in 103,991 breast cancer indiv

  2. p53 protein expression independently predicts outcome in patients with lower-risk myelodysplastic syndromes with del(5q)

    Saft, Leonie; Karimi, Mohsen; Ghaderi, Mehran; Matolcsy, András; Mufti, Ghulam J.; Kulasekararaj, Austin; Göhring, Gudrun; Giagounidis, Aristoteles; Selleslag, Dominik; Muus, Petra; Sanz, Guillermo; Mittelman, Moshe; Bowen, David; Porwit, Anna; Fu, Tommy; Backstrom, Jay; Fenaux, Pierre; MacBeth, Kyle J.; Hellström-Lindberg, Eva


    Del(5q) myelodysplastic syndromes defined by the International Prognostic Scoring System as low- or intermediate-1-risk (lower-risk) are considered to have an indolent course; however, recent data have identified a subgroup of these patients with more aggressive disease and poorer outcomes. Using deep sequencing technology, we previously demonstrated that 18% of patients with lower-risk del(5q) myelodysplastic syndromes carry TP53 mutated subclones rendering them at higher risk of progression. In this study, bone marrow biopsies from 85 patients treated with lenalidomide in the MDS-004 clinical trial were retrospectively assessed for p53 expression by immunohistochemistry in association with outcome. Strong p53 expression in ≥1% of bone marrow progenitor cells, observed in 35% (30 of 85) of patients, was significantly associated with higher acute myeloid leukemia risk (P=0.0006), shorter overall survival (P=0.0175), and a lower cytogenetic response rate (P=0.009), but not with achievement or duration of 26-week transfusion independence response. In a multivariate analysis, p53-positive immunohistochemistry was the strongest independent predictor of transformation to acute myeloid leukemia (P=0.0035). Pyrosequencing analysis of laser-microdissected cells with strong p53 expression confirmed the TP53 mutation, whereas cells with moderate expression predominantly had wild-type p53. This study validates p53 immunohistochemistry as a strong and clinically useful predictive tool in patients with lower-risk del(5q) myelodysplastic syndromes. This study was based on data from the MDS 004 trial ( identifier: NCT00179621). PMID:24682512

  3. Cytogenetic follow-up by karyotyping and fluorescence in situ hybridization: implications for monitoring patients with myelodysplastic syndrome and deletion 5q treated with lenalidomide

    Göhring, Gudrun; Giagounidis, Aristoteles; Büsche, Guntram; Hofmann, Winfried; Kreipe, Hans Heinrich; Fenaux, Pierre; Hellström-Lindberg, Eva; Schlegelberger, Brigitte


    In patients with low and intermediate risk myelodysplastic syndrome and deletion 5q (del(5q)) treated with lenalidomide, monitoring of cytogenetic response is mandatory, since patients without cytogenetic response have a significantly increased risk of progression. Therefore, we have reviewed cytogenetic data of 302 patients. Patients were analyzed by karyotyping and fluorescence in situ hybridization. In 85 patients, del(5q) was only detected by karyotyping. In 8 patients undergoing karyotypic evolution, the del(5q) and additional chromosomal aberrations were only detected by karyotyping. In 3 patients, del(5q) was only detected by fluorescence in situ hybridization, but not by karyotyping due to a low number of metaphases. Karyotyping was significantly more sensitive than fluorescence in situ hybridization in detecting the del(5q) clone. In conclusion, to optimize therapy control of myelodysplastic syndrome patients with del(5q) treated with lenalidomide and to identify cytogenetic non-response or progression as early as possible, fluorescence in situ hybridization alone is inadequate for evaluation. Karyotyping must be performed to optimally evaluate response. ( identifier: NCT01099267 and NCT00179621) PMID:21109690

  4. A p53-dependent mechanism underlies macrocytic anemia in a mouse model of human 5q- syndrome.

    Barlow, Jillian L; Drynan, Lesley F; Hewett, Duncan R; Holmes, Luke R; Lorenzo-Abalde, Silvia; Lane, Alison L; Jolin, Helen E; Pannell, Richard; Middleton, Angela J; Wong, See Heng; Warren, Alan J; Wainscoat, James S; Boultwood, Jacqueline; McKenzie, Andrew N J


    The identification of the genes associated with chromosomal translocation breakpoints has fundamentally changed understanding of the molecular basis of hematological malignancies. By contrast, the study of chromosomal deletions has been hampered by the large number of genes deleted and the complexity of their analysis. We report the generation of a mouse model for human 5q- syndrome using large-scale chromosomal engineering. Haploinsufficiency of the Cd74-Nid67 interval (containing Rps14, encoding the ribosomal protein S14) caused macrocytic anemia, prominent erythroid dysplasia and monolobulated megakaryocytes in the bone marrow. These effects were associated with defective bone marrow progenitor development, the appearance of bone marrow cells expressing high amounts of the tumor suppressor p53 and increased bone marrow cell apoptosis. Notably, intercrossing with p53-deficient mice completely rescued the progenitor cell defect, restoring common myeloid progenitor and megakaryocytic-erythroid progenitor, granulocyte-monocyte progenitor and hematopoietic stem cell bone marrow populations. This mouse model suggests that a p53-dependent mechanism underlies the pathophysiology of the 5q- syndrome.

  5. Identification of a haplotype block in the 5q31 cytokine gene cluster associated with the susceptibility to severe malaria

    Tsuchiya Naoyuki


    Full Text Available Abstract Background It has been previously demonstrated that a single nucleotide polymorphism (SNP in the IL13 promoter region, IL13 -1055T>C (rs1800925, was associated with susceptibility to severe malaria in Thais. In the present study, fine association mapping for a cytokine gene cluster including IL4, IL5, and IL13 on chromosome 5q31 was conducted using the same malaria subjects to refine the region containing a primary variant or a haplotype susceptible to severe malaria. Methods A total of 82 SNPs spanning 522 kb of the 5q31 region were analysed in 368 patients with Plasmodium falciparum malaria (203 mild malaria and 165 severe malaria patients. Results Only rs1881457 located in the promoter region of IL13, which is in linkage disequilibrium with rs1800925 (r2 = 0.73, showed a significant association with severe malaria after adjusting for multiple testing (P = 0.046 by permutation test. This SNP was in a haplotype block spanning 97 kb (from rs2069812 to rs2240032. The detected haplotype block contained the RAD50 gene and the promoter of IL13, but not the other genes. Conclusion A haplotype block in which a primary polymorphism associated with severe malaria is likely to be encoded was identified in Thai malaria patients.

  6. Variation in conserved non-coding sequences on chromosome 5q and susceptibility to asthma and atopy

    Dubchak Inna


    Full Text Available Abstract Background Evolutionarily conserved sequences likely have biological function. Methods To determine whether variation in conserved sequences in non-coding DNA contributes to risk for human disease, we studied six conserved non-coding elements in the Th2 cytokine cluster on human chromosome 5q31 in a large Hutterite pedigree and in samples of outbred European American and African American asthma cases and controls. Results Among six conserved non-coding elements (>100 bp, >70% identity; human-mouse comparison, we identified one single nucleotide polymorphism (SNP in each of two conserved elements and six SNPs in the flanking regions of three conserved elements. We genotyped our samples for four of these SNPs and an additional three SNPs each in the IL13 and IL4 genes. While there was only modest evidence for association with single SNPs in the Hutterite and European American samples (P IL4 gene (P IL13 gene was strongly associated with total IgE (P = 0.00022 and allergic sensitization to mold allergens (P = 0.00076 in the Hutterites, and more modestly associated with sensitization to molds in the European Americans and African Americans (P Conclusion These results indicate that there is overall little variation in the conserved non-coding elements on 5q31, but variation in IL4 and IL13, including possibly one SNP in a conserved element, influence asthma and atopic phenotypes in diverse populations.

  7. Trisomy 2p: Analysis of unusual phenotypic findings

    Lurie, I.W.; Ilyina, H.G.; Gurevich, D.B. [Belorussian Research Institute of Hereditary Disease, Minsk (Russian Federation)] [and others


    We present three probands with partial trisomies 2p21-23 due to ins(4;2)(q21;p21p23) pat, 2p23-pter due to t(2;4)(p23;q35)mat, and 2p21-pter due to t(2;11)(p21;q23.3)mat. More than 50 cases of partial trisomy 2p have been reviewed and some abnormalities, unusual for most other types of structural autosomal imbalance, have been found in patients with inherited forms of 2p trisomy and in their non-karyotyped sibs. Neural tube defects (anencephaly, occipital encephalocele, and spina bifida) were found in five probands and 4/6 affected non-karyotyped sibs. The only triplicated segment common to all was 2p24. Different forms of {open_quotes}broncho-pulmonary a/hypoplasia{close_quotes} (including two cases of lung agenesis) were described in four patients (overlapping triplicated segment was 2p21-p25). Three patients (with overlapping triplicated segment 2p23-p25) had diaphragmatic hernia. Abnormal rotation of the heart or L-transposition of large vessels (with or without visceral heterotaxia) was found in two infants (overlapping triplicated segment 2p23-p24). In two patients with common triplicated segment 2p22.3-p25, neuroblastoma has been described. The occurrence of all these defects may be explained either by the action of the same gene(s) mapped to 2p24 or by action of some independent factors located in different segments of the short arm. Although the latter hypothesis is much less probable, it can not be rejected at the present time. We propose the existence of a genetic system controlling surveillance of an abnormal embryo to explain the phenotypic differences between patients with the same imbalance within a family. In some {open_quotes}restrictive{close_quotes} combinations the abnormal embryos will die, although in {open_quotes}permissive{close_quotes} combinations they can survive. 47 refs., 2 figs., 3 tabs.

  8. Dramatic response in the dependency to transfusion after low doses of lenalidomide treatment in a 5q-syndrome patient: a case report.

    Dogu, Mehmet Hilmi; Sari, Ismail; Hacioglu, Sibel; Keskin, Ali


    5q-syndrome is a special subgroup of myelodysplastic syndrome in terms of follow-up and treatment. Lenalidomide is an immunomodulatory drug that is frequently used in the treatment of multiple myeloma. Some clinical studies have shown that lenalidomide treatment is effective in 5q syndrome and significantly decreases the transfusion dependency in these patients. In this paper, we would like to share a dramatic response of lowered transfusion dependency after treatment with low-dose lenalidomide in a patient who received myelodysplastic syndrome diagnosis and isolated 5q anomaly in our clinic.

  9. Early lenalidomide treatment for low and intermediate-1 International Prognostic Scoring System risk myelodysplastic syndromes with del(5q) before transfusion dependence.

    Oliva, Esther N; Lauseker, Michael; Aloe Spiriti, Maria Antonietta; Poloni, Antonella; Cortelezzi, Agostino; Palumbo, Giuseppe A; Balleari, Enrico; Sanpaolo, Grazia; Volpe, Antonio; Ricco, Alessandra; Ronco, Francesca; Alati, Caterina; D'Errigo, Maria Grazia; Santacaterina, Irene; Kündgen, Andrea; Germing, Ulrich; Latagliata, Roberto


    Lenalidomide is approved for the treatment of transfusion-dependent (TD) del(5q) myelodysplastic syndromes (MDS). However, few data are available in patients with transfusion-independent (TI) del(5q) MDS. In the first, observational, part of this 2-part study, we assessed the impact of transfusion dependence on overall survival (OS) and non-leukemic death in untreated del(5q) MDS patients who were TD (n = 136), TI with hemoglobin (Hb) ≥10 mg/dL (n = 88), or TI with Hb lenalidomide (10 mg/day) in 12 patients with TI del(5q) MDS and Hb lenalidomide (+12.5; P = 0.020). Evaluable TI patients experienced early increases in Hb levels, and all attained an erythroid response. Our findings suggest that TI patients with moderate anemia may benefit from early treatment with lenalidomide.

  10. High level of full-length cereblon mRNA in lower risk myelodysplastic syndrome with isolated 5q deletion is implicated in the efficacy of lenalidomide.

    Jonasova, Anna; Bokorova, Radka; Polak, Jaroslav; Vostry, Martin; Kostecka, Arnost; Hajkova, Hana; Neuwirtova, Radana; Siskova, Magda; Sponerova, Dana; Cermak, Jaroslav; Mikulenkova, Dana; Cervinek, Libor; Brezinova, Jana; Michalova, Kyra; Fuchs, Ota


    Downregulation of cereblon (CRBN) gene expression is associated with resistance to the immunomodulatory drug lenalidomide and poor survival outcomes in multiple myeloma (MM) patients. However, the importance of CRBN gene expression in patients with myelodysplastic syndrome (MDS) and its impact on lenalidomide therapy are not clear. In this study, we evaluate cereblon expression in mononuclear cells isolated from bone marrow [23 lower risk MDS patients with isolated 5q deletion (5q-), 37 lower risk MDS patients with chromosome 5 without the deletion of long arms (non-5q-), and 24 healthy controls] and from peripheral blood (38 patients with 5q-, 52 non-5q- patients and 25 healthy controls) to gain insight into, firstly, the role of cereblon in lower risk MDS patients with or without 5q deletion and, secondly, into the mechanisms of lenalidomide action. Patients with 5q- lower risk MDS have the highest levels of CRBN mRNA in comparison with both lower risk MDS without the deletion of long arms of chromosome 5 and healthy controls. CRBN gene expression was measured using the quantitative TaqMan real-time PCR. High levels of CRBN mRNA were detected in all lenalidomide responders during the course of therapy. A significant decrease of the CRBN mRNA level during lenalidomide treatment is associated with loss of response to treatment and disease progression. These results suggest that, similar to the treatment of MM, high levels of full-length CRBN mRNA in lower risk 5q- patients are necessary for the efficacy of lenalidomide.

  11. $^3P_2$ Superfluids Are Topological

    Mizushima, Takeshi


    We clarify the topology of the $^3P_2$ superfluidity which is expected to be realized in the cores of neutron stars and cubic odd-parity superconductors. The phase diagram includes the unitary uniaxial/biaxial nematic phases and nonunitary ferromagnetic and cyclic phases. We here show that the low-energy structures of all the phases are governed by different types of topologically protected gapless fermionic excitations: Surface Majorana fermions in nematic phases, single itinerant Majorana fermion in the ferromagnetic phase, and a quartet of itinerant Majorana fermions in the cyclic phase. Using the superfluid Fermi liquid theory, we also demonstrate that dihedral-two and -four biaxial nematic phases are thermodynamically favored in the weak coupling limit under a magnetic field. The mass acquisition of surface Majorana fermions in nematic phases is subject to symmetry.

  12. P2P overlay over Simctl

    Sicart López, Gerard Carles


    This project intends to find and implement a p2p overlay over the simctl platform. Simctl is used both for teaching and research in the network engineering department. P2P systems became an interesting area since early 2000. Researchers conducted a large amount of research in some challenging areas and, to check their experiments, several implementations and simulators were created. Over time, Internet has evolved and P2P has been widely used for file sharing, but the main structured P2P o...

  13. Lenalidomide as a disease-modifying agent in patients with del(5q) myelodysplastic syndromes: linking mechanism of action to clinical outcomes.

    Giagounidis, Aristoteles; Mufti, Ghulam J; Fenaux, Pierre; Germing, Ulrich; List, Alan; MacBeth, Kyle J


    Deletion of the long arm of chromosome 5, del(5q), is the most prevalent cytogenetic abnormality in patients with myelodysplastic syndromes (MDS). In isolation, it is traditionally associated with favorable prognosis compared with other subtypes of MDS. However, owing to the inherent heterogeneity of the disease, prognosis for patients with del(5q) MDS is highly variable depending on the presence of factors such as additional chromosomal abnormalities, >5 % blasts in the bone marrow (BM), or transfusion dependence. Over recent years, the immunomodulatory drug lenalidomide has demonstrated remarkable efficacy in patients with del(5q) MDS. Advances in the understanding of the pathogenesis of the disease have suggested that lenalidomide targets aberrant signaling pathways caused by haplosufficiency of specific genes in a commonly deleted region on chromosome 5 (e.g., SPARC, RPS14, Cdc25C, and PP2A). As a result, the agent specifically targets del(5q) clones while also promoting erythropoiesis and repopulation of the bone marrow in normal cells. This review discusses recent developments in the understanding of the mechanism of action of lenalidomide, and how this underlies favorable outcomes in patients with del(5q) MDS. In addition, we discuss how improved understanding of the mechanism of disease will facilitate clinicians' ability to predict/monitor response and identify patients at risk of relapse.

  14. Coinage metal complexes supported by a "PN(3)P" scaffold.

    Rao, Gyandshwar Kumar; Gorelsky, Serge I; Korobkov, Ilia; Richeson, Darrin


    A series of monovalent group 11 complexes, [2,6-{Ph2PNMe}2(NC5H3)]CuBr 1, [2,6-{Ph2PNMe}2(NC5H3)]CuOTf 2, [2,6-{Ph2PNMe}2(NC5H3)]AgOTf 3, and [2,6-{Ph2PNMe}2(NC5H3)](AuCl)24, supported by a neutral PN(3)P ligand have been synthesized and characterized by multinuclear NMR and single crystal X-ray diffraction studies. The variation of the coordination properties were analyzed and electronic structure calculations have been carried out to provide insight on the bonding details in these complexes. The Cu(I) complexes displayed an unusual coordination geometry with a tridentate pincer ligand and an overall four coordinate trigonal pyramidal geometry. In contrast the Ag(I) analogue displayed a bidentate κ(2)-P,P' ligation leaving the pyridyl-N atom uncoordinated and yielding a pyramidalized trigonal planar geometry around Ag. The bimetallic Au(I) complex completed the series and displayed a monodentate P-bonded ligand and a linear coordination geometry.

  15. Characterization of P2P Systems

    Stutzbach, Daniel; Rejaie, Reza

    The combination of large scale and geographically distributed nature of P2P system has led to their significant impact on the Internet. It is essential to characterize deployed P2P system for at least three reasons: (1) Accurately assessing their impact on the Internet, (2) identifying any performance bottleneck as well as any opportunity for performance improvement, (3) understanding user-driven dynamics in P2P systems. To characterize a P2P system, one needs to accurately capture snapshots of the resulting P2P overlay. This is challenging because the overlay is often large and dynamic. While the overlay is discovered by a crawler, it is changing which leads to a distorted view of the system. Capturing unbiased view of the traffic in the overlay is equally challenging because it is difficult to show that the captured behavior represent the observed behavior by all peers. In this chapter, we describe some of the fundamental problems in empirical characterization of widely deployed P2P systems. We present several examples to illustrate the effect of ad-hoc measurement/data collection on the resulting analysis/characterization. We then present two sampling techniques as a powerful approach to capture unbiased view of peer properties in a scalable fashion.

  16. The dielectronic satellites to the 2s-3p Ne-like krypton resonance lines

    Khakhalin, S.Ya. (MISDC, NPO ' ' VNIIFTRI' ' , Mendeleevo (Russian Federation)); Dyakin, V.M. (MISDC, NPO ' ' VNIIFTRI' ' , Mendeleevo (Russian Federation)); Faenov, A.Ya. (MISDC, NPO ' ' VNIIFTRI' ' , Mendeleevo (Russian Federation)); Fiedorowicz, H. (Inst. of Optoelectronics, Warsaw (Poland)); Bartnik, A. (Inst. of Optoelectronics, Warsaw (Poland)); Parys, P. (Inst. of Plasma Physics and Laser Microfusion, Warsaw (Poland)); Nilsen, J. (Lawrence Livermore National Lab., Livermore, CA (United States)); Osterheld, A. (Lawrence Livermore National Lab., Livermore, CA (United States))


    We present an analysis of dielectronic satellite spectra of 2p[sup 6]-2s2p[sup 6]3p Ne-like krypton resonance lines. The satellite structure was registered with high (better than [lambda]/[Delta][lambda] > 3500) spectral resolution in the emission of a laser irradiated gas puff target. We perform an unambiguous identification of satellite lines caused by radiative transitions from autoionizing states of sodium-like krypton ions. A total of about 20 spectral features are identified, most of them for the first time. Very good agreement between the satellite structure calculations and experimental emission spectra is obtained. (orig.).

  17. A novel unbalanced de novo translocation der(5t(4;5(q26;q21.1 in adult T-cell precursor lymphoblastic leukemia

    Kjeldsen Eigil


    Full Text Available Abstract We here describe a novel unbalanced de novo translocation der(5t(4;5(q26;q21.1 in a 39-year-old male diagnosed with acute T-cell lymphoblastic leukemia. Bone marrow (BM was massively infiltrated with 85 % highly proliferative polymorphic T-cell precursors. Immunologically, the malignant cells stained positive for CD7, CD34, intracytoplasmic CD3+, TdT + and negative for CD3 and CD5. G-banded chromosome analysis of BM cells showed the normal karyotype 46,XY[25] whereas BAC-based aCGH analysis revealed partial gain of 4q and partial loss of 5q. Multicolor karyotyping confirmed the presence of an unbalanced der(5t(4;5 as the sole structural abnormality. Subsequent high-resolution oligonucleotide-based aCGH analysis showed that the der(5t(4;5(q26;q21.1 resulted in partial trisomy of 4q26qter (117,719,015-190,613,014 and partial monosomy of 5q21.1qter (100,425,442-180,857,866 and that there was no indication of any gene disruptions resulting from the breakages. Interphase FISH analysis using BAC-based specific probes for 4q26 and 5q21.1 confirmed the breakpoints and revealed approximately 80 % abnormal cells accordingly. At 4q26 the MIR1973 gene is located centromeric to the breakpoint in the copy number neutral region and the TRAM1L1 gene is located within the gained region. At 5q21.1 the genes ST8SIA4 and MIR548p are located centromeric to the breakpoint and no known genes up to approximately 1 Mb telomeric to the breakpoint in the copy number loss region. Interestingly, only the gene ST8SIA4 at 5q21.1 have been implicated in T-cell regulation as it encodes one of the key enzymes for polysialysation of surface proteins on dendritic cells which are important regulators for T-cell proliferation. The der(5t(4;5 is thought to play a crucial role in the pathogenesis of acute T-ALL due to either gain of 4q, the loss of 5q, or deregulation of genes in proximity to the breakpoints.

  18. Fluorescence in situ hybridization for del(5q) in myelodysplasia/acute myeloid leukemia: comparison of EGR1 vs. CSF1R probes and diagnostic yield over metaphase cytogenetics alone.

    Sun, Yang; Cook, James R


    To determine the clinical utility of FISH for del(5q) in MDS/AML, we first compared FISH for 5q31 (EGR1) and 5q33 (CSF1R) in 51 myeloid neoplasms containing del(5q) by metaphase cytogenetics. Next, EGR1 FISH was compared to metaphase cytogenetics alone in 269 cases of known or suspected MDS/AML. These studies show that while metaphase cytogenetics alone can detect del(5q) in most cases, FISH is particularly useful in cases with suboptimal growth. EGR1 FISH detects del(5q) in a broad variety of myeloid neoplasms, including at least most cases of 5q- syndrome, while studies for CSF1R add little to the diagnostic yield.

  19. Association of chromosome 5q21.3 polymorphisms with the exploratory eye movement dysfunction in schizophrenia

    Ma, Yuanlin; Li, Jun; Yu, Hao; Wang, Lifang; Lu, Tianlan; Pan, Chao; Han, Yonghua; Zhang, Dai; Yue, Weihua


    Schizophrenia patients show abnormalities in many eye movement tasks. Among them, exploratory eye movements (EEM) dysfunction seems to be specific to schizophrenia. However the mechanism of EEM disturbances in schizophrenia patients remains elusive. We investigate the relationship between EEM and single nucleotide polymorphisms (SNPs) or genes to identify susceptibility loci for EEM in schizophrenia. We firstly performed EEM test, then performed a genome-wide association study (GWAS) and gene-based association study of EEM in 128 individuals with schizophrenia and 143 healthy control subjects. Comparing to healthy controls, schizophrenia patients show significant decrease in NEF (22.99 ± 3.96 vs. 26.02 ± 5.72, P <0.001), TESL (368.78 ± 123.57 vs. 603.12 ± 178.63, P <0.001), MESL (16.86 ± 5.27 vs. 24.42 ± 6.46, P <0.001), RSS (8.22 ± 1.56 vs. 10.92 ± 1.09, P <0.001), and CSS (5.06 ± 0.97 vs. 6.64 ± 0.87, P <0.001). Five SNPs of the MAN2A1, at 5q21.3, were associated with EEM abnormalities (deceased CSS) and satisfied the criteria of GWAS significance threshold. One is localized near 5’-UTR (rs17450784) and four are in intron (rs1438663, rs17162094, rs6877440 and rs10067856) of the gene. Our findings suggest that the identified loci may control the schizophrenia-related quantitative EEM trait. And the identified gene, associated with the EEM phenotype, may lead to new insights into the etiology of schizophrenia. PMID:26242244

  20. Configuration interaction calculations of positron binding to Be({sup 3}P )

    Bromley, M.W.J. [Department of Physics, San Diego State University, San Diego, CA 92182 (United States)]. E-mail:; Mitroy, J. [Faculty of Technology, Charles Darwin University, Darwin, NT 0909 (Australia)]. E-mail:


    The configuration interaction method is applied to investigate the possibility of positron binding to the metastable beryllium (1s{sup 2}2s2p {sup 3}P ) state. The largest calculation obtained an estimated energy that was unstable by 0.00014 Hartree with respect to the Ps + Be{sup +}(2s) lowest dissociation channel. It is likely that positron binding to parent states with non-zero angular momentum is inhibited by centrifugal barriers.

  1. Outcomes in RBC transfusion-dependent patients with Low-/Intermediate-1-risk myelodysplastic syndromes with isolated deletion 5q treated with lenalidomide: a subset analysis from the MDS-004 study

    Giagounidis, Aristoteles; Mufti, Ghulam J; Mittelman, Moshe; Sanz, Guillermo; Platzbecker, Uwe; Muus, Petra; Selleslag, Dominik; Beyne-Rauzy, Odile; te Boekhorst, Peter; del Cañizo, Consuelo; Guerci-Bresler, Agnès; Nilsson, Lars; Lübbert, Michael; Quesnel, Bruno; Ganser, Arnold; Bowen, David; Schlegelberger, Brigitte; Göhring, Gudrun; Fu, Tommy; Benettaib, Bouchra; Hellström-Lindberg, Eva; Fenaux, Pierre


    Objective A subset analysis of the randomised, phase 3, MDS-004 study to evaluate outcomes in patients with International Prognostic Scoring System (IPSS)-defined Low-/Intermediate (Int)-1-risk myelodysplastic syndromes (MDS) with isolated del(5q). Methods Patients received lenalidomide 10 mg/d (days 1–21; n = 47) or 5 mg/d (days 1–28; n = 43) on 28-d cycles or placebo (n = 45). From the placebo and lenalidomide 5 mg groups, 84% and 58% of patients, respectively, crossed over to lenalidomide 5 or 10 mg at 16 wk, respectively. Results Rates of red blood cell-transfusion independence (RBC-TI) ≥182 d were higher in the lenalidomide 10 mg (57.4%; P < 0.0001) and 5 mg (37.2%; P = 0.0001) groups vs. placebo (2.2%). Cytogenetic response rates (major + minor responses) were 56.8% (P < 0.0001), 23.1% (P = 0.0299) and 0%, respectively. Two-year cumulative risk of acute myeloid leukaemia progression was 12.6%, 17.4% and 16.7% in the lenalidomide 10 mg, 5 mg, and placebo groups, respectively. In a 6-month landmark analysis, overall survival was longer in lenalidomide-treated patients with RBC-TI ≥182 d vs. non-responders (P = 0.0072). The most common grade 3–4 adverse event was myelosuppression. Conclusions These data support the clinical benefits and acceptable safety profile of lenalidomide in transfusion-dependent patients with IPSS-defined Low-/Int-1-risk MDS with isolated del(5q). PMID:24813620

  2. Spectra and oscillator strengths of 3p63d9-3p53d10 and 3p63d9-3p63d84p transitions for cobalt-like Sn23+ ion

    Chen Ming-Lun; Yu Xiao-Guang


    This paper calculates the spectra and oscillator strengths for highly ionized cobalt-like Sn23+ ions 3p63d9-3p53d10,3p63d9 - 3p63d84p transitions by using a multi-configuration self-consistent field method program together with the proposed fitting formula. The calculations have a good agreement with observations.

  3. P2P Techniques for Decentralized Applications

    Pacitti, Esther


    As an alternative to traditional client-server systems, Peer-to-Peer (P2P) systems provide major advantages in terms of scalability, autonomy and dynamic behavior of peers, and decentralization of control. Thus, they are well suited for large-scale data sharing in distributed environments. Most of the existing P2P approaches for data sharing rely on either structured networks (e.g., DHTs) for efficient indexing, or unstructured networks for ease of deployment, or some combination. However, these approaches have some limitations, such as lack of freedom for data placement in DHTs, and high late

  4. Oscillator strength for 3s{sup 2}3p{sup 5}-3s3p{sup 6} in Cl-like ions

    Berrington, K.A.; Waldock, J.A. [School of Science and Mathematics, Sheffield Hallam University, Sheffield (United Kingdom); Pelan, J.C [Gatsby Computational Neuroscience Unit, University College, London (United Kingdom)


    Recent experiments have revealed discrepancies between calculated and experimental lifetimes in the lowest excited level of some chlorine-like ions. New ab initio oscillator strengths using the Breit-Pauli R-matrix method are provided for the 3s{sup 2}3p{sup 5} {sup 2}P{sup o}{sub 3/2,1/2}-3s3p{sup 6} {sup 2}S{sup e}{sub 1/2} transition in Cl-like Ar II through to Cu XIII. Our calculated lifetimes for these twelve ions are respectively 5.507, 2.077, 1.177, 0.784, 0.569, 0.438, 0.350, 0.287, 0.241, 0.206, 0.178 and 0.156 ns, and are more consistent with experiments than other calculations. (author). Letter-to-the-editor.

  5. Delayed globin synthesis leads to excess heme and the macrocytic anemia of Diamond Blackfan anemia and del(5q) myelodysplastic syndrome.

    Yang, Zhantao; Keel, Siobán B; Shimamura, Akiko; Liu, Li; Gerds, Aaron T; Li, Henry Y; Wood, Brent L; Scott, Bart L; Abkowitz, Janis L


    Diamond Blackfan anemia (DBA) and myelodysplastic syndrome (MDS) with isolated del(5q) are severe macrocytic anemias; although both are associated with impaired ribosome assembly, why the anemia occurs is not known. We cultured marrow cells from DBA (n = 3) and del(5q) MDS (n = 6) patients and determined how heme (a toxic chemical) and globin (a protein) are coordinated. We show that globin translation initiates slowly, whereas heme synthesis proceeds normally. This results in insufficient globin protein, excess heme and excess reactive oxygen species in early erythroid precursors, and CFU-E (colony-forming unit-erythroid)/proerythroblast cell death. The cells that can more rapidly and effectively export heme or can slow heme synthesis preferentially survive and appropriately mature. Consistent with these observations, treatment with 10 μM succinylacetone, a specific inhibitor of heme synthesis, improved the erythroid cell output of DBA and del(5q) MDS marrow cultures by 68 to 95% (P = 0.03 to 0.05), whereas the erythroid cell output of concurrent control marrow cultures decreased by 4 to 13%. Our studies demonstrate that erythropoiesis fails when heme exceeds globin. Our data further suggest that therapies that decrease heme synthesis (or facilitate heme export) could improve the red blood cell production of persons with DBA, del(5q) MDS, and perhaps other macrocytic anemias.

  6. Lenalidomide for the Treatment of Low- or Intermediate-1-Risk Myelodysplastic Syndromes Associated with Deletion 5q Cytogenetic Abnormality: An Evidence Review of the NICE Submission from Celgene

    H.M. Sluimer-Blommestein (Hedwig); N. Armstrong (Nigel); S. Ryder; S. Deshpande (Sohan); G. Worthy (Gill); C. Noake; R. Riemsma; J. Kleijnen (Jos); J.L. Severens (Hans); M.J. Al (Maiwenn)


    textabstractThe National Institute for Health and Care Excellence (NICE) invited the manufacturer of lenalidomide (Celgene) to submit evidence of the clinical and cost effectiveness of the drug for treating adults with myelodysplastic syndromes (MDS) associated with deletion 5q cytogenetic abnormali

  7. Multivariate time-dependent comparison of the impact of lenalidomide in lower-risk myelodysplastic syndromes with chromosome 5q deletion.

    Sánchez-García, Joaquín; Del Cañizo, Consuelo; Lorenzo, Ignacio; Nomdedeu, Benet; Luño, Elisa; de Paz, Raquel; Xicoy, Blanca; Valcárcel, David; Brunet, Salut; Marco-Betes, Victor; García-Pintos, Marta; Osorio, Santiago; Tormo, Mar; Bailén, Alicia; Cerveró, Carlos; Ramos, Fernando; Diez-Campelo, María; Such, Esperanza; Arrizabalaga, Beatriz; Azaceta, Gemma; Bargay, Joan; Arilla, María J; Falantes, José; Serrano-López, Josefina; Sanz, Guillermo F


    The impact of lenalidomide treatment on long-term outcomes of patients with lower risk myelodysplastic syndromes (MDS) and chromosome 5q deletion (del(5q)) is unclear. This study used time-dependent multivariate methodology to analyse the influence of lenalidomide therapy on overall survival (OS) and acute myeloblastic leukaemia (AML) progression in 215 patients with International Prognostic Scoring System (IPSS) low or intermediate-1 risk and del(5q). There were significant differences in several relevant characteristics at presentation between patients receiving (n = 86) or not receiving lenalidomide (n = 129). The 5-year time-dependent probabilities of OS and progression to AML were 62% and 31% for patients receiving lenalidomide and 42% and 25% for patients not receiving lenalidomide; differences were not statistically significant in multivariate analysis that included all variables independently associated with those outcomes (OS, P = 0·45; risk of AML, P = 0·31, respectively). Achievement of RBC transfusion independency (P = 0·069) or cytogenetic response (P = 0·021) after lenalidomide was associated with longer OS in multivariate analysis. These data clearly show that response to lenalidomide results in a substantial clinical benefit in lower risk MDS patients with del(5q). Lenalidomide treatment does not appear to increase AML risk in this population of patients.

  8. Switch in FGFR3 and -4 expression profile during human renal development may account for transient hypercalcemia in patients with Sotos syndrome due to 5q35 microdeletions

    Mutsaers, Henricus A M; Levtchenko, Elena N; Martinerie, Laetitia; Pertijs, Jeanne C L M; Allegaert, Karel; Devriendt, Koenraad; Masereeuw, Roos; Monnens, Leo A H; Lombès, Marc


    CONTEXT: Sotos syndrome is a rare genetic disorder with a distinct phenotypic spectrum including overgrowth and learning difficulties. Here we describe a new case of Sotos syndrome with a 5q35 microdeletion, affecting the fibroblast growth factor receptor 4 (FGFR4) gene, presenting with infantile hy

  9. Allelic methylation levels of the noncoding VTRNA2-1 located on chromosome 5q31.1 predict outcome in AML

    Treppendahl, Marianne Bach; Qiu, Xiangning; Søgaard, Alexandra;


    Deletions of chromosome 5q are associated with poor outcomes in acute myeloid leukemia (AML) suggesting the presence of tumor suppressor(s) at the locus. However, definitive identification of putative tumor suppressor genes remains controversial. Here we show that a 106-nucleotide noncoding RNA...

  10. 31 CFR 30.5 - Q-5: How does a TARP recipient comply with the requirements under § 30.4 (Q-4) of this part that...


    ... STANDARDS FOR COMPENSATION AND CORPORATE GOVERNANCE § 30.5 Q-5: How does a TARP recipient comply with the... recipient's senior risk officers any risks (including long-term as well as short-term risks) that the TARP... encourage behavior focused on short-term results and not on long-term value creation. The...

  11. A randomized phase 3 study of lenalidomide versus placebo in RBC transfusion-dependent patients with Low-/Intermediate-1-risk myelodysplastic syndromes with del5q

    Fenaux, P.; Giagounidis, A.; Selleslag, D.; Beyne-Rauzy, O.; Mufti, G.; Mittelman, M.; Muus, P.; Boekhorst, P. Te; Sanz, G.; Del Canizo, C.; Guerci-Bresler, A.; Nilsson, L.; Platzbecker, U.; Lubbert, M.; Quesnel, B.; Cazzola, M.; Ganser, A.; Bowen, D.; Schlegelberger, B.; Aul, C.; Knight, R.; Francis, J.; Fu, T.; Hellstrom-Lindberg, E.


    This phase 3, randomized, double-blind study assessed the efficacy and safety of lenalidomide in 205 red blood cell (RBC) transfusion-dependent patients with International Prognostic Scoring System Low-/Intermediate-1-risk del5q31 myelodysplastic syndromes. Patients received lenalidomide 10 mg/day o

  12. Lenalidomide: a review of its use in patients with transfusion-dependent anaemia due to low- or intermediate-1-risk myelodysplastic syndrome associated with 5q chromosome deletion.

    Syed, Yahiya Y; Scott, Lesley J


    Lenalidomide (Revlimid(®)), a thalidomide analogue, is an orally administered second generation immunomodulator with anti-angiogenic, antineoplastic, anti-inflammatory and pro-erythropoietic properties. It is approved for the treatment of patients with transfusion-dependent anaemia due to International Prognostic Scoring System low- or intermediate-1-risk myelodysplastic syndrome (MDS) associated with either chromosome 5q deletion [del(5q)] with or without additional cytogenetic abnormalities (US, Japan and Switzerland etc.), or with an isolated del(5q) cytogenetic abnormality when other therapeutic options are insufficient or inadequate (EU) [featured indication]. In a randomized, double-blind, multicentre, registrational trial (MDS-004; n = 205) in this patient population, a significantly higher proportion of lenalidomide recipients than placebo recipients achieved red blood cell transfusion independence for ≥26 consecutive weeks (primary endpoint for efficacy) and cytogenetic responses. The erythroid response to lenalidomide was accompanied by an increase in the haemoglobin levels. These efficacy outcomes are generally consistent with those seen in an earlier noncomparative registrational trial (MDS-003; n = 148). In MDS-004, lenalidomide also significantly improved health-related quality of life compared with placebo at 12 weeks. Retrospective analyses that compared outcomes between lenalidomide-treated patients with low- or intermediate-1-risk del(5q) MDS and multicentre registry cohorts showed that lenalidomide treatment did not appear to increase the risk of progression to acute myeloid leukaemia. Lenalidomide had a manageable safety profile in the registrational trials, with ≤20 % of patients discontinuing treatment because of adverse events. The most common adverse events (incidence ≥20 %) occurring in lenalidomide recipients were thrombocytopenia and neutropenia, which were generally managed by dosage reductions and/or interruptions, and

  13. Quark structure of the X(3872) and χb(3P) resonances

    Ferretti, J.; Galatà, G.; Santopinto, E.


    We discuss the nature of the χb(3P) and X(3872) mesons. Are the χb(3P)'s standard bb¯ mesons or bb¯ states with a significative continuum component? Is the X(3872) a cc¯ state with continuum coupling effects or a meson-meson molecule? To do that, we compare quark model and unquenched quark model results for the mass barycenter and splittings of the χb(3P) multiplet. Future and more precise experimental results will discriminate between the two interpretations. In the case of the X(3872), we interpret it as a cc¯ core plus higher Fock components due to the coupling to the meson-meson continuum, and thus we think that it is compatible with the meson χc1(2P), with JPC=1++. The JPC=1++ quantum numbers are in agreement with the experimental results found by the LHCb collaboration. In our view, the X(3872)'s mass is lower than the quark model's predictions because of self-energy shifts. We also provide an estimation of the open charm/bottom strong decay modes of the X(3872) and χb(3P) mesons, such as X(3872)→DD¯* and χb2(3P)→BB¯, and radiative transitions.

  14. Data Sharing in P2P Systems

    Hayek, Rabab; Raschia, Guillaume; Valduriez, Patrick; Mouaddib, Noureddine

    In this chapter, we survey P2P data sharing systems. All along, we focus on the evolution from simple file-sharing systems, with limited functionalities, to Peer Data Management Systems (PDMS) that support advanced applications with more sophisticated data management techniques. Advanced P2P applications are dealing with semantically rich data (e.g., XML documents, relational tables), using a high-level SQL-like query language. We start our survey with an overview over the existing P2P network architectures, and the associated routing protocols. Then, we discuss data indexing techniques based on their distribution degree and the semantics they can capture from the underlying data. We also discuss schema management techniques which allow integrating heterogeneous data. We conclude by discussing the techniques proposed for processing complex queries (e.g., range and join queries). Complex query facilities are necessary for advanced applications which require a high level of search expressiveness. This last part shows the lack of querying techniques that allow for an approximate query answering.

  15. Gene Expression Meta-Analysis identifies Cytokine Pathways and 5q Aberrations involved in Metastasis of ERBB2 Amplified and Basal Breast Cancer

    Thomassen, Mads; Tan, Qihua; Burton, Mark


    Background: Breast tumors have been described by molecular subtypes characterized by pervasively different gene expression profiles. The subtypes are associated with different clinical parameters and origin of precursor cells. However, the biological pathways and chromosomal aberrations that differ...... the subgroups impact metastasis. Results: We have scrutinized publicly available gene expression datasets and identified molecular subtypes in 1,394 breast tumors with outcome data. By analysis of chromosomal regions and pathways using “Gene set enrichment analysis” followed by a meta-analysis, we identified...... show that high expression of 5q14 genes and low levels of TNFR2 pathway genes were associated with poor survival in basal-like cancers. Furthermore, low expression of 5q33 genes and interleukin-12 pathway genes were associated with poor outcome exclusively in ERBB2-like tumors. Conclusion...

  16. 5q14.3 deletion neurocutaneous syndrome: Contiguous gene syndrome caused by simultaneous deletion of RASA1 and MEF2C: A progressive disease.

    Ilari, Rita; Agosta, Guillermo; Bacino, Carlos


    We report the case of a young girl who was presented with complex clinical symptoms caused by the deletion of contiguous genes: RASA1 and MEF2C, located on chromosome 5q14.3. Specifically, the diagnosis of her skin disorder and vascular malformations involving central nervous system is consistent with a RASopathy. The child's neurological manifestations are observed in most patients suffering from 5q14.3 by deletion or mutation of the MEF2C gene. A review of the literature allowed us to conclude that the contiguous deletion of genes RASA1 and MEF2C fulfills the criteria for the diagnosis of a Neurocutaneous syndrome as proposed by Carr et al. [2011]. We also assessed the penetrance of RASA1 and clinical manifestations of MEF2C according to the type of deletion. This child described presents the complete symptomatology of both deleted genes. We would also like to highlight the progression of the disorder.

  17. A mitotic recombination map proximal to the APC locus on chromosome 5q and assessment of influences on colorectal cancer risk

    Clark Susan


    Full Text Available Abstract Background Mitotic recombination is important for inactivating tumour suppressor genes by copy-neutral loss of heterozygosity (LOH. Although meiotic recombination maps are plentiful, little is known about mitotic recombination. The APC gene (chr5q21 is mutated in most colorectal tumours and its usual mode of LOH is mitotic recombination. Methods We mapped mitotic recombination boundaries ("breakpoints" between the centromere (~50 Mb and APC (~112 Mb in early colorectal tumours. Results Breakpoints were non-random, with the highest frequency between 65 Mb and 75 Mb, close to a low copy number repeat region (68–71 Mb. There were, surprisingly, few breakpoints close to APC, contrary to expectations were there constraints on tumorigenesis caused by uncovering recessive lethal alleles or if mitotic recombination were mechanistically favoured by a longer residual chromosome arm. The locations of mitotic and meiotic recombination breakpoints were correlated, suggesting that the two types of recombination are influenced by similar processes, whether mutational or selective in origin. Breakpoints were also associated with higher local G+C content. The recombination and gain/deletion breakpoint maps on 5q were not, however, associated, perhaps owing to selective constraints on APC dosage in early colorectal tumours. Since polymorphisms within the region of frequent mitotic recombination on 5q might influence the frequency of LOH, we tested the 68–71 Mb low copy number repeat and nearby tagSNPs, but no associations with colorectal cancer risk were found. Conclusion LOH on 5q is non-random, but local factors do not greatly influence the rate of LOH at APC or explain inter differential susceptibility to colorectal tumours.

  18. Association between Alcoholism and the Genetic Polymorphisms of the GABAA Receptor Genes on Chromosome 5q33-34 in Korean Population

    Park, Chul-Soo; Park, So-Young; Lee, Chul-Soon; Sohn, Jin-Wook; Hahn, Gyu-Hee; Kim, Bong-Jo


    Family, twin, and adoption studies have demonstrated that genes play an important role in the development of alcoholism. We investigated the association between alcoholism and the genetic polymorphisms of the GABAA receptor genes on chromosome 5q33-34 in Korean population. The genotype of the GABAA receptor gene polymorphisms were determined by performing polymerase chain reaction genotyping for 172 normal controls and 162 male alcoholics who are hospitalized in alcoholism treatment institute...

  19. Assignment of human sprouty 4 gene to chromosome segment 5q32∼33 and analysis of its pattern of expression

    Hua Liu; Jin-Zhong Chen; Shao-Hua Gu; Jian-Liang Dai; En-Pang Zhao; Lu Huang; Wang-Xiang Xu; Yi Xie; Yu-Min Mao


    The human sprouty 4 (SPYR4) gene was localized to chromosome band 5q32∼33 by screening the Stanford radiation hybrid G3 panel using a SPRY4-specific primer pair for PCR. Northern blot analysis revealed two different mRNAs (5 kb and 2 kb) in liver, skeletal muscle, heart, lung, kidney, spleen, placenta and small intestine. Reverse transcriptase-PCR analysis showed that SPYR4 was expressed in all tested tissues to different levels.

  20. The human and mouse receptors of hyaluronan-mediated motility, RHAMM, genes (HMMR) map to human chromosome 5q33.2-qter and mouse chromosome 11

    Spicer, A.P.; McDonald, J.A. [Mayo Clinic Scottsdale, AZ (United States); Roller, M.L.; Camper, S.A. [Univ. of Michigan Medical School, Ann Arbor, MI (United States)] [and others


    The gene for the receptor for hyaluronan-mediated motility, RHAAM (designated hyaluronan-mediated motility receptor, HMMR (human) and Hmmr (mouse), for mapping purposes), was localized to human chromosome 5q33.2-qter by somatic cell and radiation hybrid analyses. Investigation of two interspecific back-crosses localized the mouse RHAMM (Hmmr) locus 18 cM from the centromere of mouse chromosome 11 within a region of synteny homology with human chromosome 5q23-q35 genes. The map position of the human RHAMM gene places it in a region comparatively rich in disease-associated genes, including those for low-frequency hearing loss, dominant limb-girdle muscular dystrophy, diastrophic dysplasia, Treacher Collins syndrome, and myeloid disorders associated with the 5q-syndrome. The RHAMM gene location and its ability to transform cells when overexpressed implicate RHAMM as a possible candidate gene in the pathogenesis of the recently described t(5;14)(q33-q34;q11) acute lymphoblastic leukemias. 18 refs., 1 fig.

  1. CCQE, 2p2h excitations and \

    Nieves, J; Sánchez, F; Vacas, M J Vicente


    We analyze the MiniBooNE muon neutrino CCQE-like d\\sigma/dT_\\mu/dcos\\theta_\\mu data using a theoretical model that, among other nuclear effects, includes RPA correlations and 2p2h (multinucleon) mechanisms. These corrections turn out to be essential for the description of the data. We find that MiniBooNE CCQE-like data are fully compatible with former determinations of the nucleon axial mass M_A ~ 1.05 GeV. This is in sharp contrast with several previous analysis where anomalously large values of M_A ~ 1.4 GeV have been suggested. We also show that because of the the multinucleon mechanism effects, the algorithm used to reconstruct the neutrino energy is not adequate when dealing with quasielastic-like events. Finally, we analyze the MiniBooNE unfolded cross section, and show that it exhibits an excess (deficit) of low (high) energy neutrinos, which is an artifact of the unfolding process that ignores 2p2h mechanisms.

  2. Fine-scale mapping of the 5q11.2 breast cancer locus reveals at least three independent risk variants regulating MAP3K1

    Glubb, Dylan M; Maranian, Mel J; Michailidou, Kyriaki


    Genome-wide association studies (GWASs) have revealed SNP rs889312 on 5q11.2 to be associated with breast cancer risk in women of European ancestry. In an attempt to identify the biologically relevant variants, we analyzed 909 genetic variants across 5q11.2 in 103,991 breast cancer individuals an...

  3. Where are $\\chi_{cJ}(3P)$?

    Chen, Dian-Yong


    In the present work, we propose the $Y(4140)$ as the $\\chi_{c1}(3P)$ state by studying the $\\chi_{c1} \\pi^+ \\pi^-$ invariant mass spectrum of the $B\\to K \\chi_{c1} \\pi^+ \\pi^-$ process. In the $D\\bar{D}$ invariant mass spectrum of the $B\\to K D\\bar{D}$ process, we find a new resonance with the mass and width to be $ (4083.0 \\pm 5.0) $ and $ (24.1 \\pm 15.4) $ MeV, respectively, which could be a good candidate of the $\\chi_{c0}(3P)$ state. The theoretical investigations on the decay behaviors of the $\\chi_{cJ}(3P)$ in the present work support the assignments of the $Y(4140)$ and $Y(4080)$ as the $\\chi_{c1}(3P)$ and $\\chi_{c0}(3P)$ states, respectively. In addition, the $\\chi_{c2}(3P)$ state is predicted to be a very narrow state. The results in the present work could be tested by further experiments in the LHCb and forthcoming Belle II.

  4. Localized Multistreams for P2P Streaming

    Majed Alhaisoni


    Full Text Available Streaming video over the Internet, including cellular networks, has now become a commonplace. Network operators typically use multicasting or variants of multiple unicasting to deliver streams to the user terminal in a controlled fashion. P2P streaming is an emerging alternative, which is theoretically more scalable but suffers from other issues arising from the dynamic nature of the system. Users' terminals become streaming nodes but they are not constantly connected. Another issue is that they are based on logical overlays, which are not optimized for the physical underlay infrastructure. An important proposition is to find effective ways to increase the resilience of the overlay whilst at the same time not conflicting with the network. In this article we look at the combination of two techniques, redundant streaming and locality awareness, in the context of both live and video-on-demand streaming. We introduce a new technique and assess it via a comparative, simulation-based study. We find that redundancy affects network utilization only marginally if traffic is kept at the edges via localization techniques.

  5. Lenalidomide with or without erythropoietin in transfusion-dependent erythropoiesis-stimulating agent-refractory lower-risk MDS without 5q deletion.

    Toma, A; Kosmider, O; Chevret, S; Delaunay, J; Stamatoullas, A; Rose, C; Beyne-Rauzy, O; Banos, A; Guerci-Bresler, A; Wickenhauser, S; Caillot, D; Laribi, K; De Renzis, B; Bordessoule, D; Gardin, C; Slama, B; Sanhes, L; Gruson, B; Cony-Makhoul, P; Chouffi, B; Salanoubat, C; Benramdane, R; Legros, L; Wattel, E; Tertian, G; Bouabdallah, K; Guilhot, F; Taksin, A L; Cheze, S; Maloum, K; Nimuboma, S; Soussain, C; Isnard, F; Gyan, E; Petit, R; Lejeune, J; Sardnal, V; Renneville, A; Preudhomme, C; Fontenay, M; Fenaux, P; Dreyfus, F


    After failure of erythropoiesis-stimulating agents (ESAs), lenalidomide (LEN) yields red blood cell (RBC) transfusion independence (TI) in 20-30% of lower-risk non-del5q myelodysplastic syndrome (MDS). Several observations suggest an additive effect of ESA and LEN in this situation. We performed a randomized phase III study in 131 RBC transfusion-dependent (TD, median transfusion requirement six RBC units per 8 weeks) lower-risk ESA-refractory non-del5q MDS. Patients received LEN alone, 10 mg per day, 21 days per 4 weeks (L arm) or LEN (same schedule) + erythropoietin (EPO) beta, 60,000 U per week (LE arm). In an intent-to-treat (ITT) analysis, erythroid response (HI-E, IWG 2006 criteria) after four treatment cycles (primary end point) was 23.1% (95% CI 13.5-35.2) in the L arm and 39.4% (95% CI 27.6-52.2) in the LE arm (P=0.044), while RBC-TI was reached in 13.8 and 24.2% of the patients in the L and LE arms, respectively (P=0.13). Median response duration was 18.1 and 15.1 months in the L and LE arms, respectively (P=0.47). Side effects were moderate and similar in the two arms. Low baseline serum EPO level and a G polymorphism of CRBN gene predicted HI-E. Combining LEN and EPO significantly improves erythroid response over LEN alone in lower-risk non-del5q MDS patients with anemia resistant to ESA.

  6. Diagnosis of del(5q MDS, 14 years after JAK-2 positive PV appearance: complete remission of both diseases with lenalidomide monotherapy

    Antonella Vaccarino


    Full Text Available This is the report of the clinical case of a patient who presents the association of a JAK-2 positive chronic myeloproliferative neoplasia to a subsequent 5q- myelodysplastic syndrome, developed after about 14 years from the first diagnosis. Patient’s symptoms had rapidly worsened, and she became transfusion-dependent. Therapy with low-dose Lenalidomide quickly reduced the splenomegaly, and completely brought white cells counts, haemoglobin and platelets back to normal.  After more than one year from start, blood cell count is still normal. As far as we know this is the first case of an effective treatment with Lenalidomide reported in this clinical setting.

  7. Diagnosis of del(5q) MDS, 14 Years after JAK-2 Positive PV Appearance: Complete Remission of both Diseases with Lenalidomide Monotherapy.

    Vaccarino, Antonella; Dogliotti, Irene; Marletto, Fabio; Demarchi, Andrea; Bazzan, Mario


    This is the report of the clinical case of a patient who presents the association of a JAK-2 positive chronic myeloproliferative neoplasia to a subsequent 5q- myelodysplastic syndrome, developed after about 14 years from the first diagnosis. Patient's symptoms had rapidly worsened, and she became transfusion-dependent. Therapy with low-dose Lenalidomide quickly reduced the splenomegaly and completely brought white cells counts, haemoglobin, and platelets back to normal. After more than one year from the start, blood cell count is still normal. As far as we know, this is the first case of an effective treatment with Lenalidomide reported in this clinical setting.

  8. The ViP2P Platform: XML Views in P2P

    Karanasos, Konstantinos; Manolescu, Ioana; Zoupanos, Spyros


    The growing volumes of XML data sources on the Web or produced by enterprises, organizations etc. raise many performance challenges for data management applications. In this work, we are concerned with the distributed, peer-to-peer management of large corpora of XML documents, based on distributed hash table (or DHT, in short) overlay networks. We present ViP2P (standing for Views in Peer-to-Peer), a distributed platform for sharing XML documents based on a structured P2P network infrastructure (DHT). At the core of ViP2P stand distributed materialized XML views, defined by arbitrary XML queries, filled in with data published anywhere in the network, and exploited to efficiently answer queries issued by any network peer. ViP2P allows user queries to be evaluated over XML documents published by peers in two modes. First, a long-running subscription mode, when a query can be registered in the system and receive answers incrementally when and if published data matches the query. Second, queries can also be asked...

  9. Chromosome 5q candidate genes in coeliac disease: genetic variation at IL4, IL5, IL9, IL13, IL17B and NR3C1.

    Ryan, A W; Thornton, J M; Brophy, K; Daly, J S; McLoughlin, R M; O'Morain, C; Abuzakouk, M; Kennedy, N P; Stevens, F M; Feighery, C; Kelleher, D; McManus, R


    Genetic predisposition to coeliac disease (CD) is determined primarily by alleles at the HLA-DQB locus, and evidence exists implicating other major histocompatibility complex-linked genes (6p21) and the CTLA4 locus on chromosome 2q33. In addition, extensive family studies have provided strong, reproducible evidence for a susceptibility locus on chromosome 5q (CELIAC2). However, the gene responsible has not been identified. We have assayed genetic variation at the IL4, IL5, IL9, IL13, IL17B and NR3C1 (GR) loci, all of which are present on chromosome 5q and have potential or demonstrated involvement in autoimmune and/or inflammatory disease, in a sample of 409 CD cases and 355 controls. Thirteen single nucleotide polymorphisms were chosen on the basis of functional relevance, prior disease association and, where possible, prior knowledge of the haplotype variation present in European populations. There were no statistically significant allele or haplotype frequency differences between cases and controls. Therefore, these results provide no evidence that these loci are associated with CD in this sample population.

  10. Narrowing the position of the Treacher Collins syndrome locus to a small interval between three new microsatellite markers at 5q32-33. 1

    Dixon, M.J.; Dixon, J. (Univ. of Manchester (United Kingdom)); Houseal, T.; Klinger, K.; Landes, G.M. (Integrated Genetics, Inc., Framingham, MA (United States)); Bhatt, M.; Ward, D.C. (Yale Univ. School of Medicine, New Haven (United States))


    Treacher Collins syndrome (TCOF1) is an autosomal dominant disorder of craniofacial development, the features of which include conductive hearing loss and cleft palate. The TCOF1 locus has been localized to chromosome 5q32-33.2. In the present study the authors have used the combined techniques of genetic linkage analysis and fluorescence in situ hybridization (FISH) to more accurately define the TCOF1 critical region. Cosmids IG90 and SPARC, which map to distal 5q, encompass two and one hypervariable microsatellite markers, respectively. The heterozygosity values of these three markers range from .72 to .81. Twenty-two unrelated TCOF1 families have been analyzed for linkage to these markers. There is strong evidence demonstrating linkage to all three markers, the strongest support for positive linkage being provided by haplotyping those markers at the locus encompassed by the cosmid IG90 (Z[sub max]= 19.65; 0 = .010). FISH to metaphase chromosomes and interphase nuclei established that IG90 lies centromeric to SPARC. This information combined with the data generated by genetic linkage analysis demonstrated that the TCOF1 locus is closely flanked proximally by IG90 and distally by SPARC. 30 refs., 2 figs., 4 tabs.

  11. A physical map of 15 loci on human chromosome 5q23-q33 by two-color fluorescence in situ hybridization

    Saltman, D.L.; Dolganov, G.M. (Genelabs Inc. Redwood City, CA (United States)); Warrington, J.A.; Wasmuth, J.J. (Univ. of California, Irvine (United States)); Lovett, M. (Univ. of Texas Southwestern Medical Center, Dallas (United States))


    The q23-q33 region of human chromosome 5 encodes a large number of growth factors, growth factor receptors, and hormone/neurotransmitter receptors. This is also the general region into which several disease genes have been mapped, including diastrophic dysplasia, Treacher Collins syndrome, hereditary startle disease, the myeloid disorders that are associated with the 5q-syndrome, autosomal-dominant forms of hereditary deafness, and limb girdle muscular dystrophy. The authors have developed a framework physical map of this region using cosmid clones isolated from the Los Alamos arrayed chromosome 5-specific library. Entry points into this library included 14 probes to genes within this interval and one anonymous polymorphic marker locus. A physical map has been constructed using fluorescence in situ hybridization of these cosmids on metaphase and interphase chromosomes, and this is in good agreement with the radiation hybrid map of the region. The derived order of loci across the region is cen-IL4-IL5-IRF1-IL3-IL9-EGR1-CD14-FGFA-GRL-D5S207-ADRB2-SPARC-RPS14-CSF1R-ADRA1, and the total distance spanned by these loci is approximately 15 Mb. The framework map, genomic clones, and contig expansion within 5q23-q33 should provide valuable resources for the eventual isolation of the clinically relevant loci that reside in this region. 31 refs., 3 figs., 2 tabs.

  12. Ypq3p-dependent histidine uptake by the vacuolar membrane vesicles of Saccharomyces cerevisiae.

    Manabe, Kunio; Kawano-Kawada, Miyuki; Ikeda, Koichi; Sekito, Takayuki; Kakinuma, Yoshimi


    The vacuolar membrane proteins Ypq1p, Ypq2p, and Ypq3p of Saccharomyces cerevisiae are known as the members of the PQ-loop protein family. We found that the ATP-dependent uptake activities of arginine and histidine by the vacuolar membrane vesicles were decreased by ypq2Δ and ypq3Δ mutations, respectively. YPQ1 and AVT1, which are involved in the vacuolar uptake of lysine/arginine and histidine, respectively, were deleted in addition to ypq2Δ and ypq3Δ. The vacuolar membrane vesicles isolated from the resulting quadruple deletion mutant ypq1Δypq2Δypq3Δavt1Δ completely lost the uptake activity of basic amino acids, and that of histidine, but not lysine and arginine, was evidently enhanced by overexpressing YPQ3 in the mutant. These results suggest that Ypq3p is specifically involved in the vacuolar uptake of histidine in S. cerevisiae. The cellular level of Ypq3p-HA(3) was enhanced by depletion of histidine from culture medium, suggesting that it is regulated by the substrate.

  13. Main: 1M2P [RPSD[Archive

    Full Text Available 1M2P トウモロコシ Corn Zea mays L. Casein Kinase Ii, Alpha Chain Name=Ack2; Zea Mays Mole...LVGRHSRKPWLKFMNADNQHLVSPEAIDFLDKLLRYDHQERLTALEAMTHPYFQQVRAAENSRTRA corn_1M2P.jpg ...

  14. L-Leucine improves the anaemia in models of Diamond Blackfan anaemia and the 5q- syndrome in a TP53-independent way.

    Narla, Anupama; Payne, Elspeth M; Abayasekara, Nirmalee; Hurst, Slater N; Raiser, David M; Look, A Thomas; Berliner, Nancy; Ebert, Benjamin L; Khanna-Gupta, Arati


    Haploinsufficiency of ribosomal proteins (RPs) and upregulation of the tumour suppressor TP53 have been shown to be the common basis for the anaemia observed in Diamond Blackfan anaemia and 5q- myelodysplastic syndrome. We previously demonstrated that treatment with L-Leucine resulted in a marked improvement in anaemia in disease models. To determine if the L-Leucine effect was Tp53-dependent, we used antisense MOs to rps19 and rps14 in zebrafish; expression of tp53 and its downstream target cdkn1a remained elevated following L-leucine treatment. We confirmed this observation in human CD34+ cells. L-Leucine thus alleviates anaemia in RP-deficient cells in a TP53-independent manner.

  15. Myelodysplastic disorders carrying both isolated del(5q) and JAK2(V617F) mutation: concise review, with focus on lenalidomide therapy.

    Musto, Pellegrino; Simeon, Vittorio; Guariglia, Roberto; Bianchino, Gabriella; Grieco, Vitina; Nozza, Filomena; La Rocca, Francesco; Marziano, Gioacchino; Lalinga, Anna Vittoria; Fabiani, Emiliano; Voso, Maria Teresa; Scaravaglio, Patrizia; Mecucci, Cristina; D'Arena, Giovanni


    The concomitant presence of del(5q) and JAK2(V617F) mutation is an infrequent event which occurs in rare patients with peculiar cytogenetic, molecular, morphological and clinical features, resembling those of both myelodysplastic syndromes and myeloproliferative neoplasms. Lenalidomide may induce rapid, profound, and long-lasting responses in a subset of these patients. However, the mechanism(s) by which the drug acts in these conditions remain not completely elucidated. A new case report and a review of all cases published so far in this setting are provided. Furthermore, the possibility of categorizing - from a clinical, pathological, and biological point of view - for at least some of these patients as a potential distinct entity is discussed.

  16. Myelodysplastic disorders carrying both isolated del(5q) and JAK2V617F mutation: concise review, with focus on lenalidomide therapy

    Musto, Pellegrino; Simeon, Vittorio; Guariglia, Roberto; Bianchino, Gabriella; Grieco, Vitina; Nozza, Filomena; La Rocca, Francesco; Marziano, Gioacchino; Lalinga, Anna Vittoria; Fabiani, Emiliano; Voso, Maria Teresa; Scaravaglio, Patrizia; Mecucci, Cristina; D’Arena, Giovanni


    The concomitant presence of del(5q) and JAK2V617F mutation is an infrequent event which occurs in rare patients with peculiar cytogenetic, molecular, morphological and clinical features, resembling those of both myelodysplastic syndromes and myeloproliferative neoplasms. Lenalidomide may induce rapid, profound, and long-lasting responses in a subset of these patients. However, the mechanism(s) by which the drug acts in these conditions remain not completely elucidated. A new case report and a review of all cases published so far in this setting are provided. Furthermore, the possibility of categorizing – from a clinical, pathological, and biological point of view – for at least some of these patients as a potential distinct entity is discussed. PMID:24966686

  17. The gene for calcium-modulating cyclophilin ligand (CAMLG) is located on human Chromosome 5q23 and a syntenic region of mouse chromosome 13

    Bram, R.J.; Valentine, V.; Shapiro, D.N. [St. Jude Children`s Research Hospital, Memphis, TN (United States)]|[Univ. of Tennessee, Memphis, TN (United States)] [and others


    The CAMLG gene encodes a novel cyclophilin B-binding protein called calcium-modulating cyclophilin ligand, which appears to be involved in the regulation of calcium signaling in T lymphocytes and other cells. The murine homolog, Caml, was localized by interspecific backcross analysis in the middle of chromosome 13. By fluorescence in situ hybridization, this gene was localized to human chromosome 5 in a region (q23) known to be syntenic to mouse chromosome 13. These results provide further evidence supporting the extensive homology between human chromosome 5q and mouse chromosome 13. In addition, the results will provide a basis for further evaluation of cytogenetic anomalies that may contribute to inherited defects in calcium signaling or immune system function. 15 refs., 2 figs.

  18. Is the X(3915) the chi_{c0}(2P)?

    Olsen, Stephen Lars


    The Particle Data Group has assigned the X(3915) meson, an omega J/psi mass peak seen in B-> omega J/psi decays and in two-photon fusion reactions gamma-gamma->omega J/psi, as the chi_{c0}(2P), the 2^3P_0 charmonium state. Here it is shown that if the X(3915) is the chi_{c0}(2P), the measured strength of the gamma-gamma->X(3915) signal implies an upper limit on the branching fraction Bf(chi_{c0}(2P)->omega J/psi)14.3% lower limit derived for the same quantity from the B-> K X(3915) decay rate. Also, the absence any signal for X(3915)-> D0 D0-bar in B+ -> K+ D0 D0-bar decays is used to establish the limit Bf(X(3915)->D0 D0-bar) omega J/psi). This contradicts expectations that chi_{c0}(2P) decays to D0 D0-bar would be a dominant process, while decays to omega J/psi, which are Okubo-Zweig-Iizuka suppressed, would be relatively rare. These, plus reasons given earlier by Guo and Meissner, raise serious doubts about the X(3915)=chi_{c0}(2P) assignment.

  19. Myelodysplastic disorders carrying both isolated del(5q and JAK2V617F mutation: concise review, with focus on lenalidomide therapy

    Musto P


    Full Text Available Pellegrino Musto,1 Vittorio Simeon,2 Roberto Guariglia,3 Gabriella Bianchino,4 Vitina Grieco,4 Filomena Nozza,4 Francesco La Rocca,2 Gioacchino Marziano,1 Anna Vittoria Lalinga,5 Emiliano Fabiani,6 Maria Teresa Voso,6 Patrizia Scaravaglio,7 Cristina Mecucci,8 Giovanni D'Arena31Scientific Direction, 2Laboratory of Preclinical and Translational Research, 3Unit of Hematology and Stem Cell Transplantation, 4Laboratory of Clinical Research and Advanced Diagnostics, 5Pathology Unit, IRCCS, Centro di Riferimento Oncologico della Basilicata, Rionero in Vulture, Italy; 6Department of Hematology, Universita Cattolica del Sacro Cuore, Rome, Italy; 7Laboratory of Internal Medicine and Hematology, S Luigi Gonzaga Hospital, Orbassano, Italy; 8Hematology and Bone Marrow Transplantation Unit, University of Perugia, Perugia, ItalyAbstract: The concomitant presence of del(5q and JAK2V617F mutation is an infrequent event which occurs in rare patients with peculiar cytogenetic, molecular, morphological and clinical features, resembling those of both myelodysplastic syndromes and myeloproliferative neoplasms. Lenalidomide may induce rapid, profound, and long-lasting responses in a subset of these patients. However, the mechanism(s by which the drug acts in these conditions remain not completely elucidated. A new case report and a review of all cases published so far in this setting are provided. Furthermore, the possibility of categorizing – from a clinical, pathological, and biological point of view – for at least some of these patients as a potential distinct entity is discussed.Keywords: myelodysplastic syndromes, myeloproliferative neoplasms, lenalidomide, del(5q, JAK2, World Health Organization

  20. Genetic and physical mapping of the Treacher Collins syndrome locus with respect to loci in the chromosome 5q3 region

    Jabs, E.W.; Li, Xiang; Coss, C.; Taylor, E. (Johns Hopkins School of Medicine, Baltimore, MD (United States)); Lovett, M. (Univ. of Texas Southwestern Medical Center, San Antonio, TX (United States)); Yamaoka, L.H.; Speer, M.C. (Duke Univ. Medical Center, Durham, NC (United States)); Cadle, R.; Hall, B. (Univ. of Kentucky, Lexington, KY (United States)); Brown, K. (Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY (United States)) (and others)


    Treacher Collins syndrome is an autosomal dominant, craniofacial developmental disorder, and its locus (TCOF1) has been mapped to chromosome 5q3. To refine the location of the gene within this region, linkage analysis was performed among the TCOF1 locus and 12 loci (IL9, FGFA, GRL, D5S207, D5S210, D5S376, CSF1R, SPARC, D5S119, D5S209, D5S527, FGFR4) in 13 Treacher Collins syndrome families. The highest maximum lod score was obtained between loci TCOF1 and D5S210 (Z = 10.52; [theta] = 0.02 [+-] 0.07). The best order, IL9-GRL-D5S207/D5S210-CSF1R-SPARC-D5S119, and genetic distances among these loci were determined in the 40 CEPH families by multipoint linkage analysis. YAC clones were used to establish the order of loci, centromere-5[prime]GRL3[prime]-D5S207-D5S210-D5S376-CSF1R-SPARC-D5S119-telomere. By combining known physical mapping data with ours, the order of chromosome 5q3 markers is centomere-IL9-FGFA-5[prime]GRL3[prime]-D5s207-D5S210-D5S376-CSF1R-SPARC-D5S119-D5S209-FGFR4-telomere. Based on this order, haplotype analysis suggests that the TCOF1 locus resides distal CSF1R and proximal to SPARC within a region less than 1 Mb in size. 29 refs., 2 figs., 2 tabs.

  1. A replication study for three nephrolithiasis loci at 5q35.3, 7p14.3 and 13q14.1 in the Japanese population.

    Yasui, Takahiro; Okada, Atsushi; Urabe, Yuji; Usami, Masayuki; Mizuno, Kentaro; Kubota, Yasue; Tozawa, Keiichi; Sasaki, Shoichi; Higashi, Yoshihito; Sato, Yoshikazu; Kubo, Michiaki; Nakamura, Yusuke; Matsuda, Koichi; Kohri, Kenjiro


    A previous genome-wide association study (GWAS) reported three novel nephrolithiasis-susceptibility loci at 5q35.3, 7p14.3 and 13q14.1. Here, we investigated the association of these loci with nephrolithiasis by using an independent Japanese sample set. We performed case-control association analysis using 601 patients with nephrolithiasis and 201 control subjects. We selected seven single-nucleotide polymorphisms (SNPs): rs12654812 and rs11746443 from 5q35.3 (RGS14-SLC34A1-PFN3-F12); rs12669187 and rs1000597 from 7p14.3 (INMT-FAM188B-AQP1); and rs7981733, rs1170155, and rs4142110 from 13q14.1 (DGKH (diacylglycerol kinase)), which were previously reported to be significantly associated with nephrolithiasis. rs12654812, rs12669187 and rs7981733 were significantly associated with nephrolithiasis after Bonferroni's correction (P=3.12 × 10(-3), odds ratio (OR)=1.43; P=6.40 × 10(-3), OR=1.57; and P=5.00 × 10(-3), OR=1.41, respectively). Meta-analysis of current and previous GWAS results indicated a significant association with nephrolithiasis (P=7.65 × 10(-15), 7.86 × 10(-14) and 1.06 × 10(-9), respectively). We observed a cumulative effect with these three SNPs; individuals with three or more risk alleles had a 5.9-fold higher risk for nephrolithiasis development than those with only one risk allele. Our findings elucidated the significance of genetic variation at these three loci in nephrolithiasis in the Japanese population.

  2. Providing VoD Streaming Using P2P Networks

    Pedro Muñoz-Gea, Juan; Malgosa-Sanahuja, Josemaria; Manzanares-Lopez, Pilar; Carlos Sanchez-Aarnoutse, Juan

    Overlays and P2P systems, initially developed to support IP multicast and file-sharing, have moved beyond that functionality. They are also proving to be key technologies for the delivery of video streaming. Recently, there have been a number of successful deployments for "live" P2P streaming. However, the question remains open whether similar P2P technologies can be used to provide VoD (Video-On-Demand) services. A P2P VoD service is more challenging to design than a P2P live streaming system because the system should allow users arriving at arbitrary times to watch (arbitrary parts of) the video.

  3. 伴有t(3;5)(q25;q34)的骨髓增生异常综合征急性髓系白血病变患者的分子特征%Molecular characterization of t (3;5)(q25;q34) in a case of acute myeloid leukemia secondary to myelodysplastic syndromes

    张悦; 邵英起; 薛永权; 王一; 张美荣; 刘旭平; 郑以州; 肖志坚


    目的 研究t(3;5)(q25;q34)的分子特征.方法 R显带染色体核型分析和染色体涂染分析确定t(3;5)(q25;q34)存在.RT-PCR法检测患者NPM-MLF1融合基因、MLF1基因表达.PCR联合序列分析检测NPM基因突变.实时定量RT-PCR(Real-time PCR)检测Evi-1,MDS1/Evi-1表达.免疫组化染色确定MLF1和NPM-MLF1蛋白细胞定位分布.Western blot法检测bcl-2蛋白表达.结果 染色体核型分析和涂染分析均证实存在染色体t(3;5)(q25;q34),NPM-MLF1融合基因阳性,经诱导治疗获得完全缓解后转为阴性.患者白血病细胞仅表达MLF1基因非编码蛋白剪接体,不表达Evi-1基因,弱表达MDS1/Evi-1.无NPM基因突变,未检出bcl-2蛋白表达.MLF1蛋白定位于细胞质,而NPM-MLF1则主要定位于胞核.结论 t(3;5)(q25;q34)是髓系肿瘤的一种少见的染色体易位,该染色体易位导致形成NPM-MLF1融合基因是其发病的分子学基础.

  4. Infantile spasm associated with 5q14.3 microdeletion syndrome: clinical and genetic characterization of a core family%5q14.3微缺失综合征导致婴儿痉挛症一家系的临床及遗传学研究

    俞冬熠; 李朔; 姜楠


    目的 分析婴儿痉挛症患儿的临床特征,并对患儿及其父母进行遗传学检测.方法 分析2014年3月接诊的1例婴儿痉挛症患儿面部特征、体格和智力发育状况.实验室检查采用常规G显带分析患儿及父母外周血染色体核型,单核苷酸多态性(SNP)基因芯片技术检测染色体微小改变,荧光原位杂交技术(FISH)验证结果,并对其父母染色体中期分裂象进行FISH检测.结果 患儿女,7岁,语言、运动、语言发育严重迟滞,生后4个月出现癫痫发作,临床诊断为“婴儿痉挛症”.体检见消瘦,面容特殊,存在刻板样不自主动作.头颅CT平扫提示“硬膜下积液”.脑电图检查提示:痫样放电频繁;高度失律.患儿及其父母常规染色体核型分析(400条带)均显示为正常核型.SNP微阵列技术检测显示该患儿染色体5q14.3区段微缺失,缺失片段为2.03 Mb,分子核型为arr5q14.3(87 538 430 ~ 89 565 757)× 1,该区段包含MEF2C基因.选择区域特异性RP11-293 L20探针,应用FISH技术对芯片结果进行验证,确认患儿5q14.3区域存在杂合缺失.父母染色体核型正常,无5q14.3区域缺失,无插入突变.结论 患儿染色体5q14.3区段发生缺失为婴儿痉挛症的病因.患儿为新发生的5q14.3微缺失综合征,父母再次生育时,建议选择SNP芯片技术进行产前诊断.%Objective To characterize the clinical feature of a child with infantile spasm,karyotype and molecular cytogenetic analyses were performed to investigate the cause of disease and choose a suitable prenatal diagnostic method for the couple with the child.Method Routine G-banding was performed to analyze the karyotype of the patient and her parents,and molecular karyotyping was performed using SNP array.Confirmation and segregation studies were performed by fluorescence in situ hybridization (FISH).Result The patient presented with severe psychomotor retardation,epilepsy,muscular hypotonia,stereotypic behavior and

  5. The 4p/sup 3/(/sup 2/P) ns, nd configurations of Se I

    Mazzoni, M. (Florence Univ. (Italy). Dipt. di Astronomia e Scienza dello Spazio)


    The photoabsorption spectrum of Se I has been photographed in the 1100-900 A wavelength region, using a flash-pyrolisys system: About twenty lines were observed, most of them for the first time. With the support of Hartree-Fock calculations they have been identified and assigned to the 4p/sup 4/->4p/sup 3/ns/sup 3/P(n=7-14) and 4p/sup 4/->4p/sup 3/nd/sup 3/D (n=5-17) series, both converging on the limit 4p/sup 3/(/sup 2/P/sub 3/2/). (orig.).

  6. Gluon fragmentation into {sup 3} P{sub J} quarkonium

    Ma, J.P.


    The functions of the gluon fragmentation into {sup 3}P{sub j} quarkonium are calculated to order {alpha}{sup 2}{sub s}. With the recent progress in analysing quarkonium systems in QCD it is possible show how the so called divergence in the limit of the zero-binding energy, which is related to P-wave quarkonia, is treated correctly in the case of fragmentation functions. The obtained fragmentation functions satisfy explicitly at the order of {alpha} {sup 2}{sub s} the Altarelli-Parisi equation and when z {yields} 0 they behave as z{sup -1} as expected. 19 refs., 7 figs.

  7. Fine-scale mapping of the 5q11.2 breast cancer locus reveals at least three independent risk variants regulating MAP3K1.

    Glubb, Dylan M; Maranian, Mel J; Michailidou, Kyriaki; Pooley, Karen A; Meyer, Kerstin B; Kar, Siddhartha; Carlebur, Saskia; O'Reilly, Martin; Betts, Joshua A; Hillman, Kristine M; Kaufmann, Susanne; Beesley, Jonathan; Canisius, Sander; Hopper, John L; Southey, Melissa C; Tsimiklis, Helen; Apicella, Carmel; Schmidt, Marjanka K; Broeks, Annegien; Hogervorst, Frans B; van der Schoot, C Ellen; Muir, Kenneth; Lophatananon, Artitaya; Stewart-Brown, Sarah; Siriwanarangsan, Pornthep; Fasching, Peter A; Ruebner, Matthias; Ekici, Arif B; Beckmann, Matthias W; Peto, Julian; dos-Santos-Silva, Isabel; Fletcher, Olivia; Johnson, Nichola; Pharoah, Paul D P; Bolla, Manjeet K; Wang, Qin; Dennis, Joe; Sawyer, Elinor J; Tomlinson, Ian; Kerin, Michael J; Miller, Nicola; Burwinkel, Barbara; Marme, Frederik; Yang, Rongxi; Surowy, Harald; Guénel, Pascal; Truong, Thérèse; Menegaux, Florence; Sanchez, Marie; Bojesen, Stig E; Nordestgaard, Børge G; Nielsen, Sune F; Flyger, Henrik; González-Neira, Anna; Benitez, Javier; Zamora, M Pilar; Arias Perez, Jose Ignacio; Anton-Culver, Hoda; Neuhausen, Susan L; Brenner, Hermann; Dieffenbach, Aida Karina; Arndt, Volker; Stegmaier, Christa; Meindl, Alfons; Schmutzler, Rita K; Brauch, Hiltrud; Ko, Yon-Dschun; Brüning, Thomas; Nevanlinna, Heli; Muranen, Taru A; Aittomäki, Kristiina; Blomqvist, Carl; Matsuo, Keitaro; Ito, Hidemi; Iwata, Hiroji; Tanaka, Hideo; Dörk, Thilo; Bogdanova, Natalia V; Helbig, Sonja; Lindblom, Annika; Margolin, Sara; Mannermaa, Arto; Kataja, Vesa; Kosma, Veli-Matti; Hartikainen, Jaana M; Wu, Anna H; Tseng, Chiu-chen; Van Den Berg, David; Stram, Daniel O; Lambrechts, Diether; Zhao, Hui; Weltens, Caroline; van Limbergen, Erik; Chang-Claude, Jenny; Flesch-Janys, Dieter; Rudolph, Anja; Seibold, Petra; Radice, Paolo; Peterlongo, Paolo; Barile, Monica; Capra, Fabio; Couch, Fergus J; Olson, Janet E; Hallberg, Emily; Vachon, Celine; Giles, Graham G; Milne, Roger L; McLean, Catriona; Haiman, Christopher A; Henderson, Brian E; Schumacher, Fredrick; Le Marchand, Loic; Simard, Jacques; Goldberg, Mark S; Labrèche, France; Dumont, Martine; Teo, Soo Hwang; Yip, Cheng Har; See, Mee-Hoong; Cornes, Belinda; Cheng, Ching-Yu; Ikram, M Kamran; Kristensen, Vessela; Zheng, Wei; Halverson, Sandra L; Shrubsole, Martha; Long, Jirong; Winqvist, Robert; Pylkäs, Katri; Jukkola-Vuorinen, Arja; Kauppila, Saila; Andrulis, Irene L; Knight, Julia A; Glendon, Gord; Tchatchou, Sandrine; Devilee, Peter; Tollenaar, Robert A E M; Seynaeve, Caroline; Van Asperen, Christi J; García-Closas, Montserrat; Figueroa, Jonine; Chanock, Stephen J; Lissowska, Jolanta; Czene, Kamila; Klevebring, Daniel; Darabi, Hatef; Eriksson, Mikael; Hooning, Maartje J; Hollestelle, Antoinette; Martens, John W M; Collée, J Margriet; Hall, Per; Li, Jingmei; Humphreys, Keith; Shu, Xiao-Ou; Lu, Wei; Gao, Yu-Tang; Cai, Hui; Cox, Angela; Cross, Simon S; Reed, Malcolm W R; Blot, William; Signorello, Lisa B; Cai, Qiuyin; Shah, Mitul; Ghoussaini, Maya; Kang, Daehee; Choi, Ji-Yeob; Park, Sue K; Noh, Dong-Young; Hartman, Mikael; Miao, Hui; Lim, Wei Yen; Tang, Anthony; Hamann, Ute; Torres, Diana; Jakubowska, Anna; Lubinski, Jan; Jaworska, Katarzyna; Durda, Katarzyna; Sangrajrang, Suleeporn; Gaborieau, Valerie; Brennan, Paul; McKay, James; Olswold, Curtis; Slager, Susan; Toland, Amanda E; Yannoukakos, Drakoulis; Shen, Chen-Yang; Wu, Pei-Ei; Yu, Jyh-Cherng; Hou, Ming-Feng; Swerdlow, Anthony; Ashworth, Alan; Orr, Nick; Jones, Michael; Pita, Guillermo; Alonso, M Rosario; Álvarez, Nuria; Herrero, Daniel; Tessier, Daniel C; Vincent, Daniel; Bacot, Francois; Luccarini, Craig; Baynes, Caroline; Ahmed, Shahana; Healey, Catherine S; Brown, Melissa A; Ponder, Bruce A J; Chenevix-Trench, Georgia; Thompson, Deborah J; Edwards, Stacey L; Easton, Douglas F; Dunning, Alison M; French, Juliet D


    Genome-wide association studies (GWASs) have revealed SNP rs889312 on 5q11.2 to be associated with breast cancer risk in women of European ancestry. In an attempt to identify the biologically relevant variants, we analyzed 909 genetic variants across 5q11.2 in 103,991 breast cancer individuals and control individuals from 52 studies in the Breast Cancer Association Consortium. Multiple logistic regression analyses identified three independent risk signals: the strongest associations were with 15 correlated variants (iCHAV1), where the minor allele of the best candidate, rs62355902, associated with significantly increased risks of both estrogen-receptor-positive (ER(+): odds ratio [OR] = 1.24, 95% confidence interval [CI] = 1.21-1.27, ptrend = 5.7 × 10(-44)) and estrogen-receptor-negative (ER(-): OR = 1.10, 95% CI = 1.05-1.15, ptrend = 3.0 × 10(-4)) tumors. After adjustment for rs62355902, we found evidence of association of a further 173 variants (iCHAV2) containing three subsets with a range of effects (the strongest was rs113317823 [pcond = 1.61 × 10(-5)]) and five variants composing iCHAV3 (lead rs11949391; ER(+): OR = 0.90, 95% CI = 0.87-0.93, pcond = 1.4 × 10(-4)). Twenty-six percent of the prioritized candidate variants coincided with four putative regulatory elements that interact with the MAP3K1 promoter through chromatin looping and affect MAP3K1 promoter activity. Functional analysis indicated that the cancer risk alleles of four candidates (rs74345699 and rs62355900 [iCHAV1], rs16886397 [iCHAV2a], and rs17432750 [iCHAV3]) increased MAP3K1 transcriptional activity. Chromatin immunoprecipitation analysis revealed diminished GATA3 binding to the minor (cancer-protective) allele of rs17432750, indicating a mechanism for its action. We propose that the cancer risk alleles act to increase MAP3K1 expression in vivo and might promote breast cancer cell survival.

  8. Fine-Scale Mapping of the 5q11.2 Breast Cancer Locus Reveals at Least Three Independent Risk Variants Regulating MAP3K1

    Glubb, Dylan M.; Maranian, Mel J.; Michailidou, Kyriaki; Pooley, Karen A.; Meyer, Kerstin B.; Kar, Siddhartha; Carlebur, Saskia; O’Reilly, Martin; Betts, Joshua A.; Hillman, Kristine M.; Kaufmann, Susanne; Beesley, Jonathan; Canisius, Sander; Hopper, John L.; Southey, Melissa C.; Tsimiklis, Helen; Apicella, Carmel; Schmidt, Marjanka K.; Broeks, Annegien; Hogervorst, Frans B.; van der Schoot, C. Ellen; Muir, Kenneth; Lophatananon, Artitaya; Stewart-Brown, Sarah; Siriwanarangsan, Pornthep; Fasching, Peter A.; Ruebner, Matthias; Ekici, Arif B.; Beckmann, Matthias W.; Peto, Julian; dos-Santos-Silva, Isabel; Fletcher, Olivia; Johnson, Nichola; Pharoah, Paul D.P.; Bolla, Manjeet K.; Wang, Qin; Dennis, Joe; Sawyer, Elinor J.; Tomlinson, Ian; Kerin, Michael J.; Miller, Nicola; Burwinkel, Barbara; Marme, Frederik; Yang, Rongxi; Surowy, Harald; Guénel, Pascal; Truong, Thérèse; Menegaux, Florence; Sanchez, Marie; Bojesen, Stig E.; Nordestgaard, Børge G.; Nielsen, Sune F.; Flyger, Henrik; González-Neira, Anna; Benitez, Javier; Zamora, M. Pilar; Arias Perez, Jose Ignacio; Anton-Culver, Hoda; Neuhausen, Susan L.; Brenner, Hermann; Dieffenbach, Aida Karina; Arndt, Volker; Stegmaier, Christa; Meindl, Alfons; Schmutzler, Rita K.; Brauch, Hiltrud; Ko, Yon-Dschun; Brüning, Thomas; Nevanlinna, Heli; Muranen, Taru A.; Aittomäki, Kristiina; Blomqvist, Carl; Matsuo, Keitaro; Ito, Hidemi; Iwata, Hiroji; Tanaka, Hideo; Dörk, Thilo; Bogdanova, Natalia V.; Helbig, Sonja; Lindblom, Annika; Margolin, Sara; Mannermaa, Arto; Kataja, Vesa; Kosma, Veli-Matti; Hartikainen, Jaana M.; Wu, Anna H.; Tseng, Chiu-chen; Van Den Berg, David; Stram, Daniel O.; Lambrechts, Diether; Zhao, Hui; Weltens, Caroline; van Limbergen, Erik; Chang-Claude, Jenny; Flesch-Janys, Dieter; Rudolph, Anja; Seibold, Petra; Radice, Paolo; Peterlongo, Paolo; Barile, Monica; Capra, Fabio; Couch, Fergus J.; Olson, Janet E.; Hallberg, Emily; Vachon, Celine; Giles, Graham G.; Milne, Roger L.; McLean, Catriona; Haiman, Christopher A.; Henderson, Brian E.; Schumacher, Fredrick; Le Marchand, Loic; Simard, Jacques; Goldberg, Mark S.; Labrèche, France; Dumont, Martine; Teo, Soo Hwang; Yip, Cheng Har; See, Mee-Hoong; Cornes, Belinda; Cheng, Ching-Yu; Ikram, M. Kamran; Kristensen, Vessela; Zheng, Wei; Halverson, Sandra L.; Shrubsole, Martha; Long, Jirong; Winqvist, Robert; Pylkäs, Katri; Jukkola-Vuorinen, Arja; Kauppila, Saila; Andrulis, Irene L.; Knight, Julia A.; Glendon, Gord; Tchatchou, Sandrine; Devilee, Peter; Tollenaar, Robert A.E.M.; Seynaeve, Caroline; Van Asperen, Christi J.; García-Closas, Montserrat; Figueroa, Jonine; Chanock, Stephen J.; Lissowska, Jolanta; Czene, Kamila; Klevebring, Daniel; Darabi, Hatef; Eriksson, Mikael; Hooning, Maartje J.; Hollestelle, Antoinette; Martens, John W.M.; Collée, J. Margriet; Hall, Per; Li, Jingmei; Humphreys, Keith; Shu, Xiao-Ou; Lu, Wei; Gao, Yu-Tang; Cai, Hui; Cox, Angela; Cross, Simon S.; Reed, Malcolm W.R.; Blot, William; Signorello, Lisa B.; Cai, Qiuyin; Shah, Mitul; Ghoussaini, Maya; Kang, Daehee; Choi, Ji-Yeob; Park, Sue K.; Noh, Dong-Young; Hartman, Mikael; Miao, Hui; Lim, Wei Yen; Tang, Anthony; Hamann, Ute; Torres, Diana; Jakubowska, Anna; Lubinski, Jan; Jaworska, Katarzyna; Durda, Katarzyna; Sangrajrang, Suleeporn; Gaborieau, Valerie; Brennan, Paul; McKay, James; Olswold, Curtis; Slager, Susan; Toland, Amanda E.; Yannoukakos, Drakoulis; Shen, Chen-Yang; Wu, Pei-Ei; Yu, Jyh-Cherng; Hou, Ming-Feng; Swerdlow, Anthony; Ashworth, Alan; Orr, Nick; Jones, Michael; Pita, Guillermo; Alonso, M. Rosario; Álvarez, Nuria; Herrero, Daniel; Tessier, Daniel C.; Vincent, Daniel; Bacot, Francois; Luccarini, Craig; Baynes, Caroline; Ahmed, Shahana; Healey, Catherine S.; Brown, Melissa A.; Ponder, Bruce A.J.; Chenevix-Trench, Georgia; Thompson, Deborah J.; Edwards, Stacey L.; Easton, Douglas F.; Dunning, Alison M.; French, Juliet D.


    Genome-wide association studies (GWASs) have revealed SNP rs889312 on 5q11.2 to be associated with breast cancer risk in women of European ancestry. In an attempt to identify the biologically relevant variants, we analyzed 909 genetic variants across 5q11.2 in 103,991 breast cancer individuals and control individuals from 52 studies in the Breast Cancer Association Consortium. Multiple logistic regression analyses identified three independent risk signals: the strongest associations were with 15 correlated variants (iCHAV1), where the minor allele of the best candidate, rs62355902, associated with significantly increased risks of both estrogen-receptor-positive (ER+: odds ratio [OR] = 1.24, 95% confidence interval [CI] = 1.21–1.27, ptrend = 5.7 × 10−44) and estrogen-receptor-negative (ER−: OR = 1.10, 95% CI = 1.05–1.15, ptrend = 3.0 × 10−4) tumors. After adjustment for rs62355902, we found evidence of association of a further 173 variants (iCHAV2) containing three subsets with a range of effects (the strongest was rs113317823 [pcond = 1.61 × 10−5]) and five variants composing iCHAV3 (lead rs11949391; ER+: OR = 0.90, 95% CI = 0.87–0.93, pcond = 1.4 × 10−4). Twenty-six percent of the prioritized candidate variants coincided with four putative regulatory elements that interact with the MAP3K1 promoter through chromatin looping and affect MAP3K1 promoter activity. Functional analysis indicated that the cancer risk alleles of four candidates (rs74345699 and rs62355900 [iCHAV1], rs16886397 [iCHAV2a], and rs17432750 [iCHAV3]) increased MAP3K1 transcriptional activity. Chromatin immunoprecipitation analysis revealed diminished GATA3 binding to the minor (cancer-protective) allele of rs17432750, indicating a mechanism for its action. We propose that the cancer risk alleles act to increase MAP3K1 expression in vivo and might promote breast cancer cell survival. PMID:25529635

  9. Research and Development of P2P Worms

    Li You; Zhi-Guang Qin


    With the development and the application of many popular peer-to-peer (P2P) systems such as eMule and BitTorrent,worms probably employ the features of these P2P networks to put them at risk.Some features,such as the local routing table and the application routing mechanism,are helpful to quickly distribute the P2P worms into the networks.This paper aims to give a comprehensive survey of P2P worms.The definition and the classification of P2P worms are discussed firstly.Then,the research and development of P2P worms, including experimental analysis,propagation modeling,and defensive approaches,are addressed and analyzed in detail.

  10. Products of the Benzene + O(3P) Reaction

    Taatjes, Craig A.; Osborn, David L.; Selby, Talitha M.; Meloni, Giovanni; Trevitt, Adam J.; Epifanovsky, Evgeny; Krylov, Anna I.; Sirjean, Baptiste; Dames, Enoch; Wang, Hai


    The gas-phase reaction of benzene with O(3P) is of considerable interest for modeling of aromatic oxidation, and also because there exist fundamental questions concerning the prominence of intersystem crossing in the reaction. While its overall rate constant has been studied extensively, there are still significant uncertainties in the product distribution. The reaction proceeds mainly through the addition of the O atom to benzene, forming an initial triplet diradical adduct, which can either dissociate to form the phenoxy radical and H atom, or undergo intersystem crossing onto a singlet surface, followed by a multiplicity of internal isomerizations, leading to several possible reaction products. In this work, we examined the product branching ratios of the reaction between benzene and O(3P) over the temperature range of 300 to 1000 K and pressure range of 1 to 10 Torr. The reactions were initiated by pulsed-laser photolysis of NO2 in the presence of benzene and helium buffer in a slow-flow reactor, and reaction products were identified by using the multiplexed chemical kinetics photoionization mass spectrometer operating at the Advanced Light Source (ALS) of Lawrence Berkeley National Laboratory. Phenol and phenoxy radical were detected and quantified. Cyclopentadiene and cyclopentadienyl radical were directly identified for the first time. Finally, ab initio calculations and master equation/RRKM modeling were used to reproduce the experimental branching ratios, yielding pressure-dependent rate expressions for the reaction channels, including phenoxy + H, phenol, cyclopentadiene + CO, which are proposed for kinetic modeling of benzene oxidation.

  11. Thermoelectric properties of polycrystalline NiSi3P4

    May, Andrew F.; McGuire, Michael A.; Wang, Hsin


    The Hall and Seebeck coefficients, electrical resistivity, and thermal conductivity of polycrystalline NiSi3P4 were characterized from 2 to 775 K. Undoped NiSi3P4 behaves like a narrow gap semiconductor, with activated electrical resistivity ρ below room temperature and a large Seebeck coefficient of ˜400 μV/K at 300 K. Attempts to substitute boron for silicon resulted in the production of extrinsic holes, yielding moderately doped semiconductor behavior with ρ increasing with increasing temperature above ˜150 K. Hall carrier densities are limited to approximately 5 × 1019 cm-3 at 200 K, which would suggest the solubility limit of boron is reached if boron is indeed incorporated into the lattice. These extrinsic samples have a Hall mobility of ˜12 cm2/V/s at 300 K, and a parabolic band equivalent effective mass of ˜3.5 times the free electron mass. At 700 K, the thermoelectric figure of merit zT reaches ˜0.1. Further improvements in thermoelectric performance would require reaching higher carrier densities, as well as a mechanism to further reduce the lattice thermal conductivity, which is ˜5 W/m/K at 700 K. Alloying in Ge results in a slight reduction of the thermal conductivity at low temperatures, with little influence observed at higher temperatures.

  12. NLO NRQCD disfavors the interpretation of X(3872) as {chi}{sub c1}(2P)

    Butenschoen, Mathias [Wien Univ. (Austria). Fakultaet fuer Physik; He, Zhi-Guo; Kniehl, Bernd A. [Hamburg Univ. (Germany). 2. Inst. fuer Theoretische Physik


    We study {chi}{sub c1}(2P) inclusive hadroproduction at next-to-leading order (NLO) within the factorization formalism of nonrelativistic quantum chromodynamics (NRQCD), including both the color-singlet {sup 3}P{sub 1}{sup [1]} and color-octet {sup 3}S{sub 1}{sup [8]} c anti c Fock states. Assuming the recently discovered X(3872) hadron to be the 2P (1{sup ++}) charmonium state, we perform a fit to the cross sections measured by the CDF, CMS, and LHCb Collaborations. We either obtain an unacceptably high value of {chi}{sup 2} or a value of vertical stroke R{sub 2P}{sup '}(0) vertical stroke incompatible with well-established potential models. We thus conclude that NLO NRQCD is incompatible with the hypothesis X(3872){identical_to}{chi}{sub c1}(2P).

  13. P2P worm detection based on application identification

    XIA Chunhe; SHI Yunping; LI Xiaojian; GAO Wei


    P2P worm exploits common vulnerabilities and spreads through peer-to-peer networks.Despite being recognized as a potential and deadly threat to the Internet recently,few relevant countermeasures are found in extant literature.Once it breaks out,a P2P worm could result in unpredictable losses.Based on propagation characteristics of the worm,this paper presents a detection method called PWD (P2P Worm Detection),which is designed based on application identification and unknown worm detection.Simulation result and LAN-environment experiment result both indicate that PWD is an effective method to detect and block P2P worms.

  14. P2P Networking and Technology Enablers in Business Applications

    Hariharan, Mahesh


    The usage of Peer to Peer Networks over the Internet has been growing by exponentially. Apartbfrom the hype surrounding P2P, it has remarkable ramifications on the way the Internet could be used. This is an area which is not explored as well as we would want to. This thesis examines the architectural differences in P2P networks and generic application domains where the principles of P2P are exploited. The usage of P2P in different business verticals and technology enablers that go along with ...

  15. Search by shortcuts in P2P scientific collaboration system


    A P2P scientific collaboration is a P2P network whose members can share documents, co-compile papers and codes, and communicate with each other instantly. From the simulation experiment we found that P2P collaboration system is a power-law network with a tail between -2 and -3.We utilized the algorithm that searches by high-degree shortcuts to improve the scalability of p2p collaboration system. The experimental result shows that the algorithm works better than random walk algorithm.

  16. The human granzyme A (HFSP, CTLA3) gene maps to 5q11-q12 and defines a new locus of the serine protease superfamily

    Fink, T.M.; Lichter, P. (Institut fuer angewandte Tumorvirologie, Heidelberg (Germany)); Wekerle, H.; Zimmer, M.; Jenne, D.E. (Max-Planck-Institut fuer Psychiatrie, Planegg-Martinsried (Germany))


    Human granzyme A (HFSP, Hanukah factor serine protease; CTLA3, cytotoxic T-lymphocyte-associated serine esterase-3), a homodimeric, trypsin-like serine protease of 60 kDa found in granules of cytolytic T cells and natural killer cells, is implicated in lymphocyte-mediated target cell lysis. It contributes to DNA fragmentation in perforin (PRF1)-lysed target cells through an unknown mechanism. The authors have isolated a cosmid clone for the functional gene of human granzyme A and established its complete exon-intron map of 10 kb. Using an 11-kb subfragment of the cloned genomic DNA as a probe, they have identified the chromosomal position of human granzyme A on 5q11-q12. Thus, the human granzyme A gene falls into a region of homology between human chromosome 5 and mouse chromosome 13, band D, where the mouse granzyme A gene has been located previously. The granzyme A gene is not linked to known members of the large superfamily of serine proteases. 20 refs., 2 figs.

  17. Long-Term Response in a Patient with del(5q Myelodysplastic Syndrome Who Discontinued Lenalidomide and Obtained a Good Response and Tolerance to Rechallenge

    Francesco Pisani


    Full Text Available Background: The introduction of the immunomodulatory drug lenalidomide has revolutionized the treatment of patients with myelodysplastic syndromes (MDS and deletion of the long arm of chromosome 5. Treatment with lenalidomide results in transfusion independence in the majority of patients, but some questions remain unresolved, among them the duration of treatment. Moreover, a number of unexpected long-term remissions in patients who stopped lenalidomide for various reasons have been observed. Case Report: We report the case of a 60-year-old Caucasian male with deletion of the long arm of chromosome 5 and International Prognostic Scoring System (IPSS-defined low-risk MDS who was treated with lenalidomide, achieving complete cytogenetic remission and erythroid response. After tapering off and interrupting the treatment, the patient relapsed and showed a new response by lenalidomide retreatment. Six years after the initial treatment, we registered a durable erythroid long-term response and good tolerance, but there was no evidence of a very profound cytogenetic response compared to using lenalidomide as a first-line treatment. Cytogenetic and fluorescence in situ hybridization together with hemoglobin level, mean corpuscular volume (MCV and vitamin B12 level helped us to monitor the patient response; during the various phases of lenalidomide treatment, MCV and vitamin B12 normalization correlated with good response. Conclusion: Lenalidomide interruption and rechallenge in some 5q- MDS patients, with low risk according to the IPSS, is safe and feasible but does not result in a profound cytogenetic response.

  18. Association between alcoholism and the genetic polymorphisms of the GABAA receptor genes on chromosome 5q33-34 in Korean population.

    Park, Chul-Soo; Park, So-Young; Lee, Chul-Soon; Sohn, Jin-Wook; Hahn, Gyu-Hee; Kim, Bong-Jo


    Family, twin, and adoption studies have demonstrated that genes play an important role in the development of alcoholism. We investigated the association between alcoholism and the genetic polymorphisms of the GABAA receptor genes on chromosome 5q33-34 in Korean population. The genotype of the GABAA receptor gene polymorphisms were determined by performing polymerase chain reaction genotyping for 172 normal controls and 162 male alcoholics who are hospitalized in alcoholism treatment institute. We found a significant association between the genetic polymorphisms of the GABAA alpha1 and GABAA alpha6 receptor gene and alcoholism. The GG genotype of the GABAA alpha1 receptor gene was associated with the onset age of alcoholism and alcohol withdrawal symptoms, and a high score on the Korean version of the ADS. However, there was no association between the genetic polymorphisms of the GABAA beta2 and gamma2 receptor gene and alcoholisms. Our finding suggest that genetic polymorphisms of the GABAA alpha1 and GABAA alpha6 receptor gene may be associated with the development of alcoholism and that the GG genotype of the GABAA alpha1 receptor gene play an important role in the development of the early onset and the severe type of alcoholism.

  19. Methods of Reprogramming to Induced Pluripotent Stem Cell Associated with Chromosomal Integrity and Delineation of a Chromosome 5q Candidate Region for Growth Advantage.

    Sobol, Maria; Raykova, Doroteya; Cavelier, Lucia; Khalfallah, Ayda; Schuster, Jens; Dahl, Niklas


    Induced pluripotent stem cells (iPSCs) have brought great promises for disease modeling and cell-based therapies. One concern related to the use of reprogrammed somatic cells is the loss of genomic integrity and chromosome stability, a hallmark for cancer and many other human disorders. We investigated 16 human iPSC lines reprogrammed by nonintegrative Sendai virus (SeV) and another 16 iPSC lines generated by integrative lentivirus for genetic changes. At early passages we detected cytogenetic rearrangements in 44% (7/16) of iPSC lines generated by lentiviral integration whereas the corresponding figure was 6% (1/16) using SeV-based delivery. The rearrangements were numerical and/or structural with chromosomes 5 and 12 as the most frequently involved chromosomes. Three iPSC lines with chromosome 5 aberrations were derived from one and the same donor. We present in this study the aberrant karyotypes including a duplication of chromosome 5q13q33 that restricts a candidate region for growth advantage. Our results suggest that the use of integrative lentivirus confers a higher risk for cytogenetic abnormalities at early passages when compared to SeV-based reprogramming. In combination, our findings expand the knowledge on acquired cytogenetic aberrations in iPSC after reprogramming and during culture.

  20. Research of P2P SIP technology%P2P SIP技术的研究

    隋晋光; 鲁士文


    在阐述P2P和SIP技术的基础上,引出了一种二者融合的新技术--P2P SIP,提出了采用P2P SIP技术系统的体系结构、工作方式,并且对P2P SIP技术的安全性问题进行了分析.

  1. Isotope shifts of the 2 p3 /2-2 p1 /2 transition in B-like ions

    Zubova, N. A.; Malyshev, A. V.; Tupitsyn, I. I.; Shabaev, V. M.; Kozhedub, Y. S.; Plunien, G.; Brandau, C.; Stöhlker, Th.


    Isotope shifts of the 2 p3 /2-2 p1 /2 transition in B-like ions are evaluated for a wide range of the nuclear charge number: Z =8 -92 . The calculations of the relativistic nuclear recoil and nuclear size effects are performed using a large-scale configuration-interaction Dirac-Fock-Sturm method. The corresponding QED corrections are also taken into account. The results of the calculations are compared with the theoretical values obtained with other methods. The accuracy of the isotope shifts of the 2 p3 /2-2 p1 /2 transition in B-like ions is significantly improved.

  2. Isotope shifts of the 2$p_{3/2}$-2$p_{1/2}$ transition in B-like ions

    Zubova, N A; Tupitsyn, I I; Shabaev, V M; Kozhedub, Y S; Plunien, G; Brandau, C; Stohlker, Th


    Isotope shifts of the 2$p_{3/2}$-2$p_{1/2}$ transition in B-like ions are evaluated for a wide range of the nuclear charge number: Z=8-92. The calculations of the relativistic nuclear recoil and nuclear size effects are performed using a large scale configuration-interaction Dirac-Fock-Sturm method. The corresponding QED corrections are also taken into account. The results of the calculations are compared with the theoretical values obtained with other methods. The accuracy of the isotope shifts of the 2$p_{3/2}$-2$p_{1/2}$ transition in B-like ions is significantly improved.

  3. Magnetocrystalline anisotropy and the magnetocaloric effect in Fe2P

    Caron, L.; Hudl, M.; Höglin, V.; Dung, N.H.; Gomez, C.P.; Sahlberg, M.; Brück, E.; Andersson, Y.; Nordblad, P.


    Magnetic and magnetocaloric properties of high-purity, giant magnetocaloric polycrystalline and single-crystalline Fe2P are investigated. Fe2P displays a moderate magnetic entropy change, which spans over 70 K and the presence of strong magnetization anisotropy proves this system is not fully itiner

  4. Lenalidomide for the Treatment of Low- or Intermediate-1-Risk Myelodysplastic Syndromes Associated with Deletion 5q Cytogenetic Abnormality: An Evidence Review of the NICE Submission from Celgene.

    Blommestein, Hedwig M; Armstrong, Nigel; Ryder, Steve; Deshpande, Sohan; Worthy, Gill; Noake, Caro; Riemsma, Rob; Kleijnen, Jos; Severens, Johan L; Al, Maiwenn J


    The National Institute for Health and Care Excellence (NICE) invited the manufacturer of lenalidomide (Celgene) to submit evidence of the clinical and cost effectiveness of the drug for treating adults with myelodysplastic syndromes (MDS) associated with deletion 5q cytogenetic abnormality, as part of the Institute's single technology appraisal (STA) process. Kleijnen Systematic Reviews Ltd (KSR), in collaboration with Erasmus University Rotterdam, was commissioned to act as the Evidence Review Group (ERG). This paper describes the company's submission, the ERG review, and the NICE's subsequent decisions. The ERG reviewed the evidence for clinical and cost effectiveness of the technology, as submitted by the manufacturer to the NICE. The ERG searched for relevant additional evidence and validated the manufacturer's decision analytic model to examine the robustness of the cost-effectiveness results. Clinical effectiveness was obtained from a three-arm, European, randomized, phase III trial among red blood cell (RBC) transfusion-dependent patients with low-/intermediate-1-risk del5q31 MDS. The primary endpoint was RBC independence for ≥26 weeks, and was reached by a higher proportion of patients in the lenalidomide 10 and 5 mg groups compared with placebo (56.1 and 42.6 vs 5.9 %, respectively; both p < 0.001). The option of dose adjustments after 16 weeks due to dose-limiting toxicities or lack of response made long-term effectiveness estimates unreliable, e.g. overall survival (OS). The de novo model of the manufacturer included a Markov state-transition cost-utility model implemented in Microsoft Excel. The base-case incremental cost-effectiveness ratio (ICER) of the manufacturer was £56,965. The ERG assessment indicated that the modeling structure represented the course of the disease; however, a few errors were identified and some of the input parameters were challenged. In response to the appraisal documentation, the company revised the economic model

  5. A Narrow and Highly Significant Linkage Signal for Severe Bipolar Disorder in the Chromosome 5q33 Region in Latin American Pedigrees

    Jasinska, A.J.; Service, S.; Jawaheer, D.; DeYoung, J.; Levinson, M.; Zhang, Z.; Kremeyer, B.; Muller, H.; Aldana, I.; Garcia, J.; Restrepo, G.; Lopez, C.; Palacio, C.; Duque, C.; Parra, M.; Vega, J.; Ortiz, D.; Bedoya, G.; Mathews, C.; Davanzo, P.; Fournier, E.; Bejarano, J.; Ramirez, M.; Ortiz, C. Araya; Araya, X.; Molina, J.; Sabatti, C.; Reus, V.; Ospina, J.; Macaya, G.; Ruiz-Linares, A.; Freimer, N.B.


    We previously reported linkage of bipolar disorder to 5q33-q34 in families from two closely related population isolates, the Central Valley of Costa Rica (CVCR) and Antioquia, Colombia (CO). Here we present follow up results from fine-scale mapping in large CVCR and CO families segregating severe bipolar disorder, BP-I, and in 343 population trios/duos from CVCR and CO. Employing densely spaced SNPs to fine map the prior linkage peak region increases linkage evidence and clarifies the position of the putative BP-I locus. We performed two-point linkage analysis with 1134 SNPs in an approximately 9 Mb region between markers D5S410 and D5S422. Combining pedigrees from CVCR and CO yields a LOD score of 4.9 at SNP rs10035961. Two other SNPs (rs7721142 and rs1422795) within the same 94 kb region also displayed LOD scores greater than 4. This linkage peak coincides with our prior microsatellite results and suggests a narrowed BP-I susceptibility regions in these families. To investigate if the locus implicated in the familial form of BP-I also contributes to disease risk in the population, we followed up the family results with association analysis in duo and trio samples, obtaining signals within 2 Mb of the peak linkage signal in the pedigrees; rs12523547 and rs267015 (P = 0.00004 and 0.00016, respectively) in the CO sample and rs244960 in the CVCR sample and the combined sample, with P = 0.00032 and 0.00016, respectively. It remains unclear whether these association results reflect the same locus contributing to BP susceptibility within the extended pedigrees. PMID:19319892

  6. Queries mining for efficient routing in P2P communities

    Ismail, Anis; Durand, Nicolas; Nachouki, Gilles; Hajjar, Mohammad


    Peer-to-peer (P2P) computing is currently attracting enormous attention. In P2P systems a very large number of autonomous computing nodes (the peers) pool together their resources and rely on each other for data and services. Peer-to-peer (P2P) Data-sharing systems now generate a significant portion of Internet traffic. Examples include P2P systems for network storage, web caching, searching and indexing of relevant documents and distributed network-threat analysis. Requirements for widely distributed information systems supporting virtual organizations have given rise to a new category of P2P systems called schema-based. In such systems each peer exposes its own schema and the main objective is the efficient search across the P2P network by processing each incoming query without overly consuming bandwidth. The usability of these systems depends on effective techniques to find and retrieve data; however, efficient and effective routing of content-based queries is a challenging problem in P2P networks. This wo...

  7. Increasing Structured P2P Protocol Resilience to Localized Attacks

    Germanus, Daniel


    The Peer-to-Peer (P2P) computing model has been applied to many application fields over the last decade. P2P protocols made their way from infamous - and frequently illicit - file sharing applications towards serious applications, e.g., in entertainment, audio/video conferencing, or critical applications like smart grid, Car-2-Car communication, or Machine-to-Machine communication. Some of the reasons for that are P2P's decentralized design that inherently provides for fault tolerance to non-...

  8. Comparing Pedophile Activity in Different P2P Systems

    Raphaël Fournier; Thibault Cholez; Matthieu Latapy; Isabelle Chrisment; Clémence Magnien; Olivier Festor; Ivan Daniloff


    International audience; Peer-to-peer (P2P) systems are widely used to exchange content over the Internet. Knowledge of pedophile activity in such networks remains limited, despite having important social consequences. Moreover, though there are different P2P systems in use, previous academic works on this topic focused on one system at a time and their results are not directly comparable. We design a methodology for comparing KAD and eDonkey, two P2P systems among the most prominent ones and ...

  9. Code wars 10 years of P2P software litigation

    Giblin, Rebecca


    Code Wars recounts the legal and technological history of the first decade of the P2P file sharing era, focusing on the innovative and anarchic ways in which P2P technologies evolved in response to decisions reached by courts with regard to their predecessors. With reference to US, UK, Canadian and Australian secondary liability regimes, this insightful book develops a compelling new theory to explain why a decade of ostensibly successful litigation failed to reduce the number, variety or availability of P2P file sharing applications - and highlights ways the law might need to change if it is

  10. Distributed Frequent Item Sets Mining over P2P Networks

    Zahra Farzanyar; Mohammadreza Kangavari


    Data intensive peer-to-peer (P2P) networks are becoming increasingly popular in applications like social networking, file sharing networks, etc. Data mining in such P2P environments is the new generation of advanced P2P applications. Unfortunately, most of the existing data mining algorithms do not fit well in such environments since they require data that can be accessed in its entirety. It also is not easy due to the requirements of online transactional data streams. In this paper, we have ...

  11. Autoionization study of the Argon 2p satellites excited near the argon 2s threshold

    Wang, H.; Glans, P.; Hemmers, O. [Univ. of Nevada, Las Vegas, NV (United States)] [and others


    The dynamics of near-threshold photoionization is a complex phenomenon in which the many-electron character of the wavefunctions plays an important role. According to generalized time-independent resonant scattering theory, the transition matrix element from an initial state to a final state is the summation of the amplitudes of direct photoionization and an indirect term in which intermediate states are involved and the resonant behavior is embedded. Studies of the interference effects of intermediate states have been explored in the cases where the direct term is negligible. In the present work, electron time-of-flight spectra of the Ar 2p satellites were measured at two angles (magic and 0{degrees}) in the dipole plane with the exciting photon energy tuned in the vicinity of the Ar 2s threshold. For excitation far below or above the 2s threshold, the 2p satellites spectrum is dominated by 3p to np shakeup contributions upon the ionization of a 2p electron.

  12. Why X(3915) is so narrow as a $\\chi_{c0}(2P)$ state

    Jiang, Yue; Wang, Tianhong; Ju, Wan-Li


    New resonance X(3915) was identified as the charmonium $\\chi_{c0}(2P)$ by BABAR Collaboration, but there seems still open question of this assignment: why its full width is so narrow? To answer this question, we calculate the Okubo-Zweig-Iizuka (OZI) allowed strong decays $X(3915)\\to D \\bar D$, where X(3915) is assigned as a $\\chi_{c0}(2P)$ state, and estimate its full width in the cooperating framework of $^3/!P_0^{}$ model and the Bethe-Salpeter (BS) method using the Mandelstam Formalism, during which non-perturbative QCD effects of the hadronic matrix elements are well considered by overlapping integral over the relativistic Salpeter wave functions of the initial and finial states. We find the node structure of $\\chi_{c0}(2P)$ wave function resulting in the narrow width of X(3915) and show the dependence of the decay width on the variation of the initial mass of X(3915). We point out that the rate of $\\frac{\\Gamma(X(3915)\\to D^+ D^-)}{\\Gamma(X(3915)\\to D^0 \\bar D^0)}$ is crucial to confirm whether X(3915) ...

  13. Health-related quality of life outcomes of lenalidomide in transfusion-dependent patients with Low- or Intermediate-1-risk myelodysplastic syndromes with a chromosome 5q deletion: results from a randomized clinical trial.

    Revicki, D.A.; Brandenburg, N.A.; Muus, P.; Yu, R.; Knight, R.; Fenaux, P.


    We report health-related quality of life (HRQL) outcomes assessed using the Functional Assessment of Cancer Therapy-Anemia (FACT-An) among 167 RBC transfusion-dependent patients with IPSS Low-/Intermediate-1-risk del5q31 MDS treated with lenalidomide versus placebo in a randomized phase 3 clinical t

  14. Outcomes in RBC transfusion-dependent patients with Low-/Intermediate-1-risk myelodysplastic syndromes with isolated deletion 5q treated with lenalidomide: A subset analysis from the MDS-004 study

    A. Giagounidis (Aristoteles); G.J. Mufti (Ghulam); M. Mittelman (Moshe); G. Sanz (Guillermo); U. Platzbecker (Uwe); P. Muus (P.); D. Selleslag; O. Beyne-Rauzy (Odile); P.A.W. te Boekhorst (Peter); C. del Cañizo (Consuelo); A. Guerci-Bresler (Agnes); L. Nilsson (Lars); M. Lübbert (Michael); B. Quesnel (Bruno); A. Ganser (Arnold); D. Bowen (David); B. Schlegelberger (Brigitte); G. Göhring (Gudrun); T. Fu (Tommy); B. Benettaib (Bouchra); E. Hellström-Lindberg (Eva); P. Fenaux (Pierre)


    textabstractObjective: A subset analysis of the randomised, phase 3, MDS-004 study to evaluate outcomes in patients with International Prognostic Scoring System (IPSS)-defined Low-/Intermediate (Int)-1-risk myelodysplastic syndromes (MDS) with isolated del(5q). Methods: Patients received lenalidomid

  15. Improving P2P live-content delivery using SVC

    Schierl, T.; Sánchez, Y.; Hellge, C.; Wiegand, T.


    P2P content delivery techniques for video transmission have become of high interest in the last years. With the involvement of client into the delivery process, P2P approaches can significantly reduce the load and cost on servers, especially for popular services. However, previous studies have already pointed out the unreliability of P2P-based live streaming approaches due to peer churn, where peers may ungracefully leave the P2P infrastructure, typically an overlay networks. Peers ungracefully leaving the system cause connection losses in the overlay, which require repair operations. During such repair operations, which typically take a few roundtrip times, no data is received from the lost connection. While taking low delay for fast-channel tune-in into account as a key feature for broadcast-like streaming applications, the P2P live streaming approach can only rely on a certain media pre-buffer during such repair operations. In this paper, multi-tree based Application Layer Multicast as a P2P overlay technique for live streaming is considered. The use of Flow Forwarding (FF), a.k.a. Retransmission, or Forward Error Correction (FEC) in combination with Scalable video Coding (SVC) for concealment during overlay repair operations is shown. Furthermore the benefits of using SVC over the use of AVC single layer transmission are presented.

  16. Characterization of P2P IPTV Traffic: Scaling Analysis

    Silverston, Thomas; Salamatian, Kave


    P2P IPTV applications arise on the Internet and will be massively used in the future. It is expected that P2P IPTV will contribute to increase the overall Internet traffic. In this context, it is important to measure the impact of P2P IPTV on the networks and to characterize this traffic. During the 2006 FIFA World Cup, we performed an extensive measurement campaign. We measured network traffic generated by broadcasting soccer games by the most popular P2P IPTV applications, namely PPLive, PPStream, SOPCast and TVAnts. From the collected data, we characterized the P2P IPTV traffic structure at different time scales. To the best of our knowledge, this is the first work, which presents a complete multiscale analysis of the P2P IPTV traffic. Our observations show that the network traffic has not the same scale behavior whether the applications use TCP or UDP. For all the applications, the download traffic is different from the upload traffic and the signaling traffic has an impact on the download traffic.

  17. Network Awareness in P2P-TV Applications

    Traverso, Stefano; Leonardi, Emilio; Mellia, Marco; Meo, Michela

    The increasing popularity of applications for video-streaming based on P2P paradigm (P2P-TV) is raising the interest of both broadcasters and network operators. The former see a promising technology to reduce the cost of streaming content over the Internet, while offering a world-wide service. The latter instead fear that the traffic offered by these applications can grow without control, affecting other services, and possibly causing network congestion and collapse. The “Network-Aware P2P-TV Application over Wise Networks” FP7 project aims at studying and developing a novel P2P-TV application offering the chance to broadcast high definition video to broadcasters and to carefully manage the traffic offered by peers to the network, therefore avoiding worries to Internet providers about network overload. In such context, we design a simulator to evaluate performance of different P2P-TV solutions, to compare them both considering end-users’ and network providers’ perspectives, such as quality of service perceived by subscribers and link utilization. In this paper, we provide some results that show how effective can be a network aware P2P-TV system.

  18. P2P Data Management in Mobile Wireless Sensor Network

    Nida Sahar Sayeda


    Full Text Available The rapid growth in wireless technologies has made wireless communication an important source for transporting data across different domains. In the same way, there are possibilities of many potential applications that can be deployed using WSNs (Wireless Sensor Networks. However, very limited applications are deployed in real life due to the uncertainty and dynamics of the environment and scare resources. This makes data management in WSN a challenging area to find an approach that suits its characteristics. Currently, the trend is to find efficient data management schemes using evolving technologies, i.e. P2P (Peer-to-Peer systems. Many P2P approaches have been applied in WSNs to carry out the data management due to similarities between WSN and P2P. With the similarities, there are differences too that makes P2P protocols inefficient in WSNs. Furthermore, to increase the efficiency and to exploit the delay tolerant nature of WSNs, where ever possible, the mobile WSNs are gaining importance. Thus, creating a three dimensional problem space to consider, i.e. mobility, WSNs and P2P. In this paper, an efficient algorithm is proposed for data management using P2P techniques for mobile WSNs. The real world implementation and deployment of proposed algorithm is also presented

  19. Four novel Loci (19q13, 6q24, 12q24, and 5q14 influence the microcirculation in vivo.

    M Kamran Ikram


    Full Text Available There is increasing evidence that the microcirculation plays an important role in the pathogenesis of cardiovascular diseases. Changes in retinal vascular caliber reflect early microvascular disease and predict incident cardiovascular events. We performed a genome-wide association study to identify genetic variants associated with retinal vascular caliber. We analyzed data from four population-based discovery cohorts with 15,358 unrelated Caucasian individuals, who are members of the Cohort for Heart and Aging Research in Genomic Epidemiology (CHARGE consortium, and replicated findings in four independent Caucasian cohorts (n  =  6,652. All participants had retinal photography and retinal arteriolar and venular caliber measured from computer software. In the discovery cohorts, 179 single nucleotide polymorphisms (SNP spread across five loci were significantly associated (p<5.0×10(-8 with retinal venular caliber, but none showed association with arteriolar caliber. Collectively, these five loci explain 1.0%-3.2% of the variation in retinal venular caliber. Four out of these five loci were confirmed in independent replication samples. In the combined analyses, the top SNPs at each locus were: rs2287921 (19q13; p  =  1.61×10(-25, within the RASIP1 locus, rs225717 (6q24; p = 1.25×10(-16, adjacent to the VTA1 and NMBR loci, rs10774625 (12q24; p  =  2.15×10(-13, in the region of ATXN2,SH2B3 and PTPN11 loci, and rs17421627 (5q14; p = 7.32×10(-16, adjacent to the MEF2C locus. In two independent samples, locus 12q24 was also associated with coronary heart disease and hypertension. Our population-based genome-wide association study demonstrates four novel loci associated with retinal venular caliber, an endophenotype of the microcirculation associated with clinical cardiovascular disease. These data provide further insights into the contribution and biological mechanisms of microcirculatory changes that underlie cardiovascular

  20. A sib-pair analysis study of 15 candidate genes in French families with morbid obesity: indication for linkage with islet 1 locus on chromosome 5q.

    Clément, K; Dina, C; Basdevant, A; Chastang, N; Pelloux, V; Lahlou, N; Berlan, M; Langin, D; Guy-Grand, B; Froguel, P


    As part of an ongoing search for susceptibility genes in obese families, we performed linkage analyses in 101 French families between qualitative and quantitative traits related to morbid obesity and polymorphisms located in or near 15 candidate genes whose products are involved in body weight regulation. These included cholecystokinin A and B receptors (CCK-AR and CCK-BR), glucagon-like peptide 1 receptor (GLP-1R), the LIM/homeodomain islet-1 gene (Isl-1), the caudal-type homeodomain 3 (CDX-3), the uncoupling protein 1 (UCP-1), the beta3-adrenoceptor (beta3-AR), the fatty acid-binding protein 2 (FABP-2), the hormone-sensitive lipase (HSL), the lipoprotein lipase (LPL), the apoprotein-C2 (apo-C2), the insulin receptor substrate-1 (IRS-1), the peroxisome proliferator-activated receptor-gamma (PPAR-gamma), tumor necrosis factor-alpha (TNF-alpha), and the liver carnitine palmitoyltransferase-1 (CPT-1). Phenotypes related to obesity such as BMI, adult life body weight gain, fasting leptin, insulin, fasting glycerol, and free fatty acids were used for nonparametric sib-pair analyses. A weak indication for linkage was obtained between the Isl-1 locus and obesity status defined by a z score over one SD of BMI (n = 226 sib pairs, pi = 0.54 +/- 0.02, P = 0.03). Moreover, a suggestive indication for linkage was found between the Isl-1 locus and BMI and leptin values (P = 0.001 and 0.0003, respectively) and leptin adjusted for BMI (P = 0.0001). Multipoint analyses for leptin trait with Isl-1 and two flanking markers (D5S418 and D5S407) showed that the logarithm of odds (LOD) score is 1.73, coinciding with the Isl-1 locus. Although marginally positive indications for linkage in subgroups of families were found with IRS-1, CPT-1, and HSL loci, our data suggested that these genes are not major contributors to obesity. Whether an obesity susceptibility gene (Isl-1 itself or another nearby gene) lies on chromosome 5q should be determined by further analyses.

  1. Phosphatidic acid and phosphoinositides facilitate liposome association of Yas3p and potentiate derepression of ARE1 (alkane-responsive element one)-mediated transcription control.

    Kobayashi, Satoshi; Hirakawa, Kiyoshi; Horiuchi, Hiroyuki; Fukuda, Ryouichi; Ohta, Akinori


    In the n-alkane assimilating yeast Yarrowia lipolytica, the expression of ALK1, encoding a cytochrome P450 that catalyzes terminal mono-oxygenation of n-alkanes, is induced by n-alkanes. The transcription of ALK1 is regulated by a heterocomplex that comprises the basic helix-loop-helix transcription activators, Yas1p and Yas2p, and binds to alkane-responsive element 1 (ARE1) in the ALK1 promoter. An Opi1 family transcription repressor, Yas3p, represses transcription by binding to Yas2p. Yas3p localizes in the nucleus when Y. lipolytica is grown on glucose but localizes to the endoplasmic reticulum (ER) upon the addition of n-alkanes. In this study, we showed that recombinant Yas3p binds to the acidic phospholipids, phosphatidic acid (PA) and phosphoinositides (PIPs), in vitro. The ARE1-mediated transcription was enhanced in vivo in mutants defective in an ortholog of the Saccharomyces cerevisiae gene PAH1, encoding PA phosphatase, and in an ortholog of SAC1, encoding PIP phosphatase in the ER. Truncation mutation analyses for Yas3p revealed two regions that bound to PA and PIPs. These results suggest that the interaction with acidic phospholipids is important for the n-alkane-induced association of Yas3p with the ER membrane.

  2. Behavioural Correlation for Detecting P2P Bots

    Al-Hammadi, Yousof


    In the past few years, IRC bots, malicious programs which are remotely controlled by the attacker through IRC servers, have become a major threat to the Internet and users. These bots can be used in different malicious ways such as issuing distributed denial of services attacks to shutdown other networks and services, keystrokes logging, spamming, traffic sniffing cause serious disruption on networks and users. New bots use peer to peer (P2P) protocols start to appear as the upcoming threat to Internet security due to the fact that P2P bots do not have a centralized point to shutdown or traceback, thus making the detection of P2P bots is a real challenge. In response to these threats, we present an algorithm to detect an individual P2P bot running on a system by correlating its activities. Our evaluation shows that correlating different activities generated by P2P bots within a specified time period can detect these kind of bots.

  3. Managing Linguistic Data Summaries in Advanced P2P Applications

    Hayek, Rabab; Raschia, Guillaume; Valduriez, Patrick; Mouaddib, Noureddine

    As the amount of stored data increases, data localization techniques become no longer sufficient in P2P systems. A practical approach is to rely on compact database summaries rather than raw database records, whose access is costly in large P2P systems. In this chapter, we describe a solution for managing linguistic data summaries in advanced P2P applications which are dealing with semantically rich data. The produced summaries are synthetic, multidimensional views over relational tables. The novelty of this proposal relies on the double summary exploitation in distributed P2P systems. First, as semantic indexes, they support locating relevant nodes based on their data descriptions. Second, due to their intelligibility, these summaries can be directly queried and thus approximately answer a query without the need for exploring original data. The proposed solution consists first in defining a summary model for hierarchical P2P systems. Second, appropriate algorithms for summary creation and maintenance are presented. A query processing mechanism, which relies on summary querying, is then proposed to demonstrate the benefits that might be obtained from summary exploitation.

  4. Determinants of Default in P2P Lending.

    Carlos Serrano-Cinca

    Full Text Available This paper studies P2P lending and the factors explaining loan default. This is an important issue because in P2P lending individual investors bear the credit risk, instead of financial institutions, which are experts in dealing with this risk. P2P lenders suffer a severe problem of information asymmetry, because they are at a disadvantage facing the borrower. For this reason, P2P lending sites provide potential lenders with information about borrowers and their loan purpose. They also assign a grade to each loan. The empirical study is based on loans' data collected from Lending Club (N = 24,449 from 2008 to 2014 that are first analyzed by using univariate means tests and survival analysis. Factors explaining default are loan purpose, annual income, current housing situation, credit history and indebtedness. Secondly, a logistic regression model is developed to predict defaults. The grade assigned by the P2P lending site is the most predictive factor of default, but the accuracy of the model is improved by adding other information, especially the borrower's debt level.

  5. Determinants of Default in P2P Lending.

    Serrano-Cinca, Carlos; Gutiérrez-Nieto, Begoña; López-Palacios, Luz


    This paper studies P2P lending and the factors explaining loan default. This is an important issue because in P2P lending individual investors bear the credit risk, instead of financial institutions, which are experts in dealing with this risk. P2P lenders suffer a severe problem of information asymmetry, because they are at a disadvantage facing the borrower. For this reason, P2P lending sites provide potential lenders with information about borrowers and their loan purpose. They also assign a grade to each loan. The empirical study is based on loans' data collected from Lending Club (N = 24,449) from 2008 to 2014 that are first analyzed by using univariate means tests and survival analysis. Factors explaining default are loan purpose, annual income, current housing situation, credit history and indebtedness. Secondly, a logistic regression model is developed to predict defaults. The grade assigned by the P2P lending site is the most predictive factor of default, but the accuracy of the model is improved by adding other information, especially the borrower's debt level.

  6. A Framework For Concept Drifting P2P Traffic Identification

    Guanghui Yan


    Full Text Available Identification of network traffic using port-based or payload-based analysis is becoming increasing difficult with many Peer-to-Peer (P2P application using dynamic ports, masquerading techniques, and encryption to avoid detection. To overcome this problem, several machine learning technique were proposed to classify P2P traffics. But in the real P2P network environment, new communities of peers often attend and old communities of peers often leave. It requires the identification methods to be capable of coping with concept drift, and updating the model incrementally. In this paper, we present a concept-adapting algorithm CluMC which is based on streaming data mining techniques to identify P2P applications in Internet traffic. The CluMC use micro-cluster structures which contain potential micro-cluster structures and outlier micro-cluster structures to classify the P2P traffic and discover the concept drift with limited memory. Our performance study over a number of real data sets that we captured at a main gateway router demonstrates the effectiveness and efficiency of our method.

  7. Measurement of the spin-forbidden decay rate (3s3d)1D2¿(3s3p)3 P2,1 in 24Mg

    Therkildsen, K. T.; Jensen, Brian Bak; Ryder, C. P.;


    We have measured the spin-forbidden decay rate from (3s3d)D12¿(3s3p)P32,1 in M24g atoms trapped in a magneto-optical trap. The total decay rate, summing up both exit channels (3s3p)P31 and (3s3p)P32 , yields 196±10s-1 in excellent agreement with resent relativistic many-body calculations of Porsev...

  8. On Using Seeders for P2P Live Streaming

    Mathieu, Fabien


    Seeders (peers that do not request anything but contribute to the system) are a powerful concept in peer-to-peer (P2P). They allow to leverage the capacities of a P2P system. While seeding is a natural idea for filesharing or video-on-demand applications, it seems somehow counter-intuitive in the context of live streaming. This paper aims at describing the feasibility and performance of P2P live seeding. After a formal definition of "live seeding" and efficiency, we consider the theoretical performance of systems where the overhead is neglected. We then propose a linear overhead model and extend the results for this model, for a single seeder and for a set of seeders as well (it is not always possible to perfectly aggregate individual efficiencies in a given system).

  9. Compromising Tor Anonymity Exploiting P2P Information Leakage

    Manils, Pere; Blond, Stevens Le; Kaafar, Mohamed Ali; Castelluccia, Claude; Legout, Arnaud; Dabbous, Walid


    Privacy of users in P2P networks goes far beyond their current usage and is a fundamental requirement to the adoption of P2P protocols for legal usage. In a climate of cold war between these users and anti-piracy groups, more and more users are moving to anonymizing networks in an attempt to hide their identity. However, when not designed to protect users information, a P2P protocol would leak information that may compromise the identity of its users. In this paper, we first present three attacks targeting BitTorrent users on top of Tor that reveal their real IP addresses. In a second step, we analyze the Tor usage by BitTorrent users and compare it to its usage outside of Tor. Finally, we depict the risks induced by this de-anonymization and show that users' privacy violation goes beyond BitTorrent traffic and contaminates other protocols such as HTTP.

  10. Model of Controlling the Hubs in P2P Networks

    Yuhua Liu


    Full Text Available Research into the hubs in Peer-to-Peer (P2P networks, and present a new method to avoid generating the hubs in the networks by controlling the logical topology structure of P2P networks. We firstly introduce the controlling ideas about hierarchizing the hubs. Then, we disclose and interpret the controlling model, and give out the concrete method to carry it out. Finally, we validate our controlling model via simulations and the simulation results demonstrate that our work is effective to control the hubs in P2P networks. Thus, this model can improve the network competence to defend against coordinated attacks, promote the network robustness, and ensure the network would develop continually and healthily.

  11. PROSE: Proactive, Selective CDN Participation for P2P Streaming

    Zhi-Hui Lv; Li-Jiang Chen; Jie Wu; Da Deng; Si-Jia Huang; Yi Huang


    Many production peer-to-peer (P2P) streaming systems use content delivery networks (CDN) to protect the user's quality of experiences.Thus,how to efficiently utilize the capacity of CDN (e.g.,which peers receive services from the CDN nodes) is a problem of practical significance.Existing solutions adopt a passive,on-demand approach,which is inefficient iu utilizing CDN resources.In this paper,we propose PROSE,a simple,novel scheme to achieve proactive,selective CDN participation for P2P streaming.PROSE introduces novel concepts such as choke point expansion nodes/super nodes and leads to efficient,light-weighted,and distributed algorithms to identify and serve these nodes using CDN.Our experimental results show that PROSE achieves at least 10%~25% performance improvement and 2~4 times overhead reduction compared with existing general CDN-P2P-hybrid schemes.

  12. Market Design for a P2P Backup System

    Seuken, Sven; Charles, Denis; Chickering, Max; Puri, Sidd

    Peer-to-peer (P2P) backup systems are an attractive alternative to server-based systems because the immense costs of large data centers can be saved by using idle resources on millions of private computers instead. This paper presents the design and theoretical analysis of a market for a P2P backup system. While our long-term goal is an open resource exchange market using real money, here we consider a system where monetary transfers are prohibited. A user who wants to backup his data must in return supply some of his resources (storage space, upload and download bandwidth) to the system.We propose a hybrid P2P architecture where all backup data is transferred directly between peers, but a dedicated server coordinates all operations and maintains meta-data. We achieve high reliability guarantees while keeping our data replication factor low by adopting sophisticated erasure coding technology (cf., [2]).

  13. On the {P2, P3}-Factor of Cubic Graphs

    GOU Kui-xiang; SUN Liang


    Let G = ( V, E) be a finite simple graph and Pn denote the path of order n. A spanning subgraph F is called a {P2, P3}-factor of G if each component of F is isomorphic to P2 or P3. With the path-covering method, it is proved that any connected cubic graph with at least 5 vertices has a { P2, P3 }-factor F such that | P3 (F) |≥|P2 (F) |, where P2 (F) and P3 (F) denote the set of components of P2 and P3 in F,respectively.

  14. Mobile P2P Web Services Using SIP

    Guido Gehlen


    Full Text Available Telecommunication networks and the Internet are growing together. Peer-to-Peer (P2P services which are originally offered by network providers, like telephony and messaging, are provided through VoIP and Instant Messaging (IM by Internet service providers, too. The IP Multimedia Subsystem (IMS is the answer of the telecommunication industry to this trend and aims at providing Internet P2P and multimedia services controlled by the network operators. The IMS provides mobility and session management as well as message routing, security, and billing.

  15. Association of Serum MiR-142-3p and MiR-101-3p Levels with Acute Cellular Rejection after Heart Transplantation

    Sukma Dewi, Ihdina; Hollander, Zsuzsanna; Lam, Karen K.; McManus, Janet-Wilson; Tebbutt, Scott J.; Ng, Raymond T.; Keown, Paul A.; McMaster, Robert W.; McManus, Bruce M.; Gidlöf, Olof; Öhman, Jenny


    Background Identifying non-invasive and reliable blood-derived biomarkers for early detection of acute cellular rejection in heart transplant recipients is of great importance in clinical practice. MicroRNAs are small molecules found to be stable in serum and their expression patterns reflect both physiological and underlying pathological conditions in human. Methods We compared a group of heart transplant recipients with histologically-verified acute cellular rejection (ACR, n = 26) with a control group of heart transplant recipients without allograft rejection (NR, n = 37) by assessing the levels of a select set of microRNAs in serum specimens. Results The levels of seven microRNAs, miR-142-3p, miR-101-3p, miR-424-5p, miR-27a-3p, miR-144-3p, miR-339-3p and miR-326 were significantly higher in ACR group compared to the control group and could discriminate between patients with and without allograft rejection. MiR-142-3p and miR-101-3p had the best diagnostic test performance among the microRNAs tested. Serum levels of miR-142-3p and miR-101-3p were independent of calcineurin inhibitor levels, as measured by tacrolimus and cyclosporin; kidney function, as measured by creatinine level, and general inflammation state, as measured by CRP level. Conclusion This study demonstrated two microRNAs, miR-142-3p and miR-101-3p, that could be relevant as non-invasive diagnostic tools for identifying heart transplant patients with acute cellular rejection. PMID:28125729

  16. Silane photoabsorption spectra near the Si 2p thresholds: the geometry of Si 2p excited SiH4

    张卫华; 许如清; 李家明


    Based on the multiple-scattering self-consistent-field method, we have studied the photoabsorption spectra near the Si 2p thresholds of silane. According to our calculations, the clear assignments of the inner-shell photoabsorption spectra are provided. In comparison with the high-resolution experimental spectra, the geometric structure of the Si 2p-excited SiH4** is recommended to be of a C2v symmetry. More specifically, the Si 2p-excited Si4** have two bond lengths of 2.50 a.u. and another two bond lengths of 2.77 a.u., and the corresponding two bond angles are 104.0° and 112.5° respectively.

  17. Measurement and Analysis of P2P IPTV Program Resource

    Wenxian Wang


    Full Text Available With the rapid development of P2P technology, P2P IPTV applications have received more and more attention. And program resource distribution is very important to P2P IPTV applications. In order to collect IPTV program resources, a distributed multi-protocol crawler is proposed. And the crawler has collected more than 13 million pieces of information of IPTV programs from 2009 to 2012. In addition, the distribution of IPTV programs is independent and incompact, resulting in chaos of program names, which obstructs searching and organizing programs. Thus, we focus on characteristic analysis of program resources, including the distributions of length of program names, the entropy of the character types, and hierarchy depth of programs. These analyses reveal the disorderly naming conventions of P2P IPTV programs. The analysis results can help to purify and extract useful information from chaotic names for better retrieval and accelerate automatic sorting of program and establishment of IPTV repository. In order to represent popularity of programs and to predict user behavior and popularity of hot programs over a period, we also put forward an analytical model of hot programs.

  18. P2P Domain Classification using Decision Tree

    Ismail, Anis


    In Peer-to-Peer context, a challenging problem is how to find the appropriate peer to deal with a given query without overly consuming bandwidth? Different methods proposed routing strategies of queries taking into account the P2P network at hand. This paper considers an unstructured P2P system based on an organization of peers around Super-Peers that are connected to Super-Super- Peer according to their semantic domains; By analyzing the queries log file, a predictive model that avoids flooding queries in the P2P network is constructed after predicting the appropriate Super-Peer, and hence the peer to answer the query. A challenging problem in a schema-based Peer-to-Peer (P2P) system is how to locate peers that are relevant to a given query. In this paper, architecture, based on (Super-)Peers is proposed, focusing on query routing. The approach to be implemented, groups together (Super-)Peers that have similar interests for an efficient query routing method. In such groups, called Super-Super-Peers (SSP), Su...

  19. Comparing Pedophile Activity in Different P2P Systems

    Raphaël Fournier


    Full Text Available Peer-to-peer (P2P systems are widely used to exchange content over the Internet. Knowledge of pedophile activity in such networks remains limited, despite having important social consequences. Moreover, though there are different P2P systems in use, previous academic works on this topic focused on one system at a time and their results are not directly comparable. We design a methodology for comparing KAD and eDonkey, two P2P systems among the most prominent ones and with different anonymity levels. We monitor two eDonkey servers and the KAD network during several days and record hundreds of thousands of keyword-based queries. We detect pedophile-related queries with a previously validated tool and we propose, for the first time, a large-scale comparison of pedophile activity in two different P2P systems. We conclude that there are significantly fewer pedophile queries in KAD than in eDonkey (approximately 0.09% vs. 0.25%.

  20. Treatment of refractory anemia with ring sideroblasts associated with marked thrombocytosis with lenalidomide in a patient testing negative for 5q deletion and JAK2 V617F and MPL W515K/L mutations

    Ryan Keen


    Full Text Available Refractory anemia with ring sideroblasts associated with marked thrombocytosis (RARS-T is a hematologic malignancy that often results in transfusion dependency and a hypercoagulable state. This rare disease currently lacks formal guidelines for treatment; however, various case reports have demonstrated efficacy in the use of lenalidomide. This immunomodulatory drug has shown promise in patients with 5q deletions, with reports of achieving transfusion independence and normalization of platelet counts. Herein we present the case of a 68-year-old African American woman with RARS-T who tested negative for 5q deletion and JAK2 V617F and MPL W515K/L mutations. Her treatment with lenalidomide therapy resulted in a five-year durable complete clinical response.

  1. ICL-induced miR139-3p and miR199a-3p have opposite roles in hematopoietic cell expansion and leukemic transformation.

    Alemdehy, Mir Farshid; Haanstra, Jurgen R; de Looper, Hans W J; van Strien, Paulina M H; Verhagen-Oldenampsen, Judith; Caljouw, Yvette; Sanders, Mathijs A; Hoogenboezem, Remco; de Ru, Arnoud H; Janssen, George M C; Smetsers, Stephanie E; Bierings, Marc B; van Veelen, Peter A; von Lindern, Marieke; Touw, Ivo P; Erkeland, Stefan J


    Interstrand crosslinks (ICLs) are toxic DNA lesions that cause severe genomic damage during replication, especially in Fanconi anemia pathway-deficient cells. This results in progressive bone marrow failure and predisposes to acute myeloid leukemia (AML). The molecular mechanisms responsible for these defects are largely unknown. Using Ercc1-deficient mice, we show that Trp53 is responsible for ICL-induced bone marrow failure and that loss of Trp53 is leukemogenic in this model. In addition, Ercc1-deficient myeloid progenitors gain elevated levels of miR-139-3p and miR-199a-3p with age. These microRNAs exert opposite effects on hematopoiesis. Ectopic expression of miR-139-3p strongly inhibited proliferation of myeloid progenitors, whereas inhibition of miR-139-3p activity restored defective proliferation of Ercc1-deficient progenitors. Conversely, the inhibition of miR-199a-3p functions aggravated the myeloid proliferation defect in the Ercc1-deficient model, whereas its enforced expression enhanced proliferation of progenitors. Importantly, miR-199a-3p caused AML in a pre-leukemic mouse model, supporting its role as an onco-microRNA. Target genes include HuR for miR-139-3p and Prdx6, Runx1, and Suz12 for miR-199a-3p. The latter genes have previously been implicated as tumor suppressors in de novo and secondary AML. These findings show that, in addition to TRP53-controlled mechanisms, miR-139-3p and miR-199a-3p are involved in the defective hematopoietic function of ICL-repair deficient myeloid progenitors.

  2. Syntenic homology of human unique DNA sequences within chromossome regions 5q31, 10q22, 13q32-33 and 19q13.1 in the great apes

    Vallente-Samonte Rhea U.


    Full Text Available Homologies between chromosome banding patterns and DNA sequences in the great apes and humans suggest an apparent common origin for these two lineages. The availability of DNA probes for specific regions of human chromosomes (5q31, 10q22, 13q32-33 and 19q13.1 led us to cross-hybridize these to chimpanzee (Pan troglodytes, PTR, gorilla (Gorilla gorilla, GGO and orangutan (Pongo pygmaeus, PPY chromosomes in a search for equivalent regions in the great apes. Positive hybridization signals to the chromosome 5q31-specific DNA probe were observed at HSA 5q31, PTR 4q31, GGO 4q31 and PPY 4q31, while fluorescent signals using the chromosome 10q22-specific DNA probe were noted at HSA 10q22, PTR 8q22, GGO 8q22 and PPY 7q22. The chromosome arms showing hybridization signals to the Quint-EssentialTM 13-specific DNA probe were identified as HSA 13q32-33, PTR 14q32-33, GGO 14q32-33 and PPY 14q32-33, while those presenting hybridization signals to the chromosome 19q13.1-specific DNA probe were identified as HSA 19q13.1, PTR 20q13, GGO 20q13 and PPY 20q13. All four probes presumably hybridized to homologous chromosomal locations in the apes, which suggests a homology of certain unique DNA sequences among hominoid species.

  3. Evaluation of chromosome 5 aberrations in complex karyotypes of patients with myeloid disorders reveals their contribution to dicentric and tricentric chromosomes, resulting in the loss of critical 5q regions.

    Herry, Angèle; Douet-Guilbert, Nathalie; Morel, Frédéric; Le Bris, Marie-Josée; Morice, Patrick; Abgrall, Jean François; Berthou, Christian; De Braekeleer, Marc


    Dicentric chromosomes have often been observed in complex karyotypes in previously reported studies of therapy-related myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). Fluorescence in situ hybridization (FISH) has now made the characterization of these rearrangements much easier. Dicentric and tricentric chromosomes were identified in 21 patients (9 MDS and 12 AML) among the 133 consecutive MDS/AML patients (17%) who had a structural or numerical aberration of chromosome 5 using conventional cytogenetic analysis. One third (7/21) of the patients had received alkylating drugs for a previously diagnosed cancer or chronic myeloproliferative disease. Loss of 5q material was identified in all 21 patients. One copy of the EGR1 (5q31) or the CSF1R (5q33 approximately q34) genes was lost in 20 of the 21 patients. Dicentric and tricentric chromosomes involving chromosome 5 are frequently observed in complex karyotypes among patients with de novo or therapy-related MDS/AML. They lead to deletions of various parts of the long arm of chromosome 5.

  4. Theatrical distribution and P2P movie piracy: a survey of P2P networks in Hungary using transactional data

    Bodó, B.; Lakatos, Z.


    This article examines what appears to be the most important factor shaping file sharing: the failure of traditional cultural markets to efficiently supply the demand in the online environment. Its findings are based on tracking the traffic of movies on three Hungarian P2P networks. This dataset is t

  5. Supporting Seamless Mobility for P2P Live Streaming

    Eunsam Kim


    Full Text Available With advent of various mobile devices with powerful networking and computing capabilities, the users' demand to enjoy live video streaming services such as IPTV with mobile devices has been increasing rapidly. However, it is challenging to get over the degradation of service quality due to data loss caused by the handover. Although many handover schemes were proposed at protocol layers below the application layer, they inherently suffer from data loss while the network is being disconnected during the handover. We therefore propose an efficient application-layer handover scheme to support seamless mobility for P2P live streaming. By simulation experiments, we show that the P2P live streaming system with our proposed handover scheme can improve the playback continuity significantly compared to that without our scheme.

  6. Supporting seamless mobility for P2P live streaming.

    Kim, Eunsam; Kim, Sangjin; Lee, Choonhwa


    With advent of various mobile devices with powerful networking and computing capabilities, the users' demand to enjoy live video streaming services such as IPTV with mobile devices has been increasing rapidly. However, it is challenging to get over the degradation of service quality due to data loss caused by the handover. Although many handover schemes were proposed at protocol layers below the application layer, they inherently suffer from data loss while the network is being disconnected during the handover. We therefore propose an efficient application-layer handover scheme to support seamless mobility for P2P live streaming. By simulation experiments, we show that the P2P live streaming system with our proposed handover scheme can improve the playback continuity significantly compared to that without our scheme.



    The letter proposes a three-layer manageable media distribution network system architecture called MSPnet, which is based on Session Initiation Protocol[1] and Peer to Peer (SIP P2P)technology. MSPnet performs application-level structured DHT routing and resource location among domains and unstructured ones in domain. Except for media distribution, it can be used to support a variety of P2P applications, including video broadcasting, video on demand, VoIP, etc. MSPnet is composed of three layers, namely, the signal control layer, the management layer, and the media transportation layer. The MSPnet prototype consists of the SIP server, the management server, the media server, and the node User Agent (UA). Results from a prototype experiment in a large-scale Internet environment show that MSPnet is feasible, scalable and manageable.

  8. Mobile P2P Trusted On-Demand Video Streaming

    Iyer, Thava; Rizvandi, Nikzad Babaii; Varghese, Benoy; Boreli, Roksana


    We propose to demonstrate a mobile server assisted P2P system for on-demand video streaming. Our proposed solution uses a combination of 3G and ad-hoc Wi-Fi connections, to enable mobile devices to download content from a centralised server in a way that minimises the 3G bandwidth use and cost. On the customised GUI, we show the corresponding reduction in 3G bandwidth achieved by increasing the number of participating mobile devices in the combined P2P and ad-hoc Wi- Fi network, while demonstrating the good video playout quality on each of the mobiles. We also demonstrate the implemented trust mechanism which enables mobiles to only use trusted adhoc connections. The system has been implemented on Android based smartphones.

  9. Fine Structure of the Upsilon 2p States.

    Lovelock, Dale Michael John


    The Columbia-Stony Brook (CUSB) collaboration has measured the photon spectrum resulting from the decays Upsilon (3S)togamma + hadrons using the CUSB-II BGO calorimeter. The spectrum contains the signal from the electromagnetic transitions Upsilon (3S)togammachi _{b,J=0,1,2} (2P). From this signal the masses of the chi_{b}(2P) states were determined to be 10268.0 +/- 0.8, 10255.4 +/- 0.8, and 10233.9 +/- 1.2 MeV for the J = 2, 1, and 0 states respectively. The ratio of the mass splittings, (M_{chi _{b2}} - M_{chi_{b1}} )/(M_{chi_{b1 }} - M_ {chi_{b0}}) imply that the long range confining piece of the inter-quark potential transforms as a Lorentz scalar.

  10. Addressing the P2P Bootstrap Problem for Small Networks

    Wolinsky, David Isaac; Boykin, P Oscar; Figueiredo, Renato


    P2P overlays provide a framework for building distributed applications consisting of few to many resources with features including self-configuration, scalability, and resilience to node failures. Such systems have been successfully adopted in large-scale services for content delivery networks, file sharing, and data storage. In small-scale systems, they can be useful to address privacy concerns and for network applications that lack dedicated servers. The bootstrap problem, finding an existing peer in the overlay, remains a challenge to enabling these services for small-scale P2P systems. In large networks, the solution to the bootstrap problem has been the use of dedicated services, though creating and maintaining these systems requires expertise and resources, which constrain their usefulness and make them unappealing for small-scale systems. This paper surveys and summarizes requirements that allow peers potentially constrained by network connectivity to bootstrap small-scale overlays through the use of e...

  11. P2P Networks with IP Based Communication

    Anupriya Koneru; Krishna Prasad MHM


    P2P communities can be seen as truly Distributed Computing applications in which group members communicate with one another to exchange information. The authors consider security issues in Peer to Peer Networks. For secure exchange of data between the group members the authors present a cryptography protocol and an Identity mechanism which can able to check even the Trust of the Peers based on the available reputation information. The authors are encapsulating the reputations of both the prov...

  12. Interface reactions in Mg/Zn3P2 solar cells

    Kazmerski, L. L.; Ireland, P. J.; Catalano, A.


    The composition and chemistry of the Mg/Zn3P2 interface was studied using surface analysis techniques. For higher carrier concentrations (p 10 to the 17th power/cu cm), a heterojunction occurs by the chemical interaction of the Mg with Zn3P2 to form Mg3P2. For lower carrier concentrations this reaction does not take place, and Mg diffuses into the Zn3P2. Diffusion coefficients of Mg in Zn3P2 are determined. Grain (crystalline) and grain boundary components of the diffusion coefficient are evaluated. These data compare very well with that reported using electron beam induced currents and spectral response measurements.

  13. A new PC(sp(3))P ligand and its coordination chemistry with low-valent iron, cobalt and nickel complexes.

    Zhu, Gengyu; Li, Xiaoyan; Xu, Guoqiang; Wang, Lin; Sun, Hongjian


    A new PC(sp(3))P ligand N,N'-bis(diphenylphosphino)dipyrromethane [PCH2P] (1) was prepared and its iron, cobalt and nickel chemistry was explored. Two pincer-type complexes [PCHP]Fe(H)(PMe3)2 (2) and [PCHP]Co(PMe3)2 (4) were synthesized in the reaction of with Fe(PMe3)4 and Co(Me)(PMe3)4. 1 reacted with Co(PMe3)4 and Ni(PMe3)4 to afford Co(0) and Ni(0) complexes [PCH2P]Co(PMe3)2 (3) and [PCH2P]Ni(PMe3)2 (5). The structures of complexes 2-5 were determined by X-ray diffraction.

  14. Atomic scattering in the diffraction limit: electron transfer in keV Li+-Na(3s, 3p) collisions

    Poel, Mike van der; Nielsen, C.V.; Rybaltover, M.;


    . This setup yields a momentum resolution of 0.12 an, an order of magnitude better angular resolution than previous measurements on this system. This enables us to clearly resolve Fraunhofer-type diffraction patterns in the angle DCS. In particular, the angular width of the ring structure is given by the ratio...... of the de Broglie wavelength lambda(dB) = 150 fm at a velocity v = 0.20 au and the effective atomic diameter for electron capture 2R = 20 au. Parallel AO and MO semiclassical coupled-channel calculations of the Na(3s, 3p) --> Li(2s, 2p) state-to-state collision amplitudes have been performed, and quantum...

  15. Random walk search in unstructured P2P

    Jia Zhaoqing; You Jinyuan; Rao Ruonan; Li Minglu


    Unstructured P2P has power-law link distribution, and the random walk in power-law networks is analyzed. The analysis results show that the probability that a random walker walks through the high degree nodes is high in the power-law network, and the information on the high degree nodes can be easily found through random walk. Random walk spread and random walk search method (RWSS) is proposed based on the analysis result. Simulation results show that RWSS achieves high success rates at low cost and is robust to high degree node failure.

  16. Efficient Search in P2P File Sharing System

    Xiao Bo; Jin Wei; Hou Mengshu


    A new routing algorithm of peer-to-peer file sharing system with routing indices was proposed, in which a node forwards a query to neighbors that are more likely to have answers based on its statistics. The proposed algorithm was tested by creating a P2P simulator and varying the input parameters, and was compared to the search algorithms using flooding (FLD) and random walk (RW). The result shows that with the proposed design, the queries are routed effectively, the network flows are reduced remarkably, and the peer-to-peer file sharing system gains a good expansibility.

  17. Role of 2p-2h MEC excitations in superscaling

    De Pace, A. E-mail:; Nardi, M.; Alberico, W.M.; Donnelly, T.W.; Molinari, A


    Following recent studies of inclusive electron scattering from nuclei at high energies which focused on two-nucleon emission mediated by meson-exchange currents, in this work the superscaling behavior of such contributions is investigated. Comparisons are made with existing data below the quasielastic peak where at high momentum transfers scaling of the second kind is known to be excellent and scaling of the first kind is good, in the proximity of the peak where both 1p-1h and 2p-2h contributions come into play, and above the peak where inelasticity becomes important and one finds scaling violations of the two kinds.

  18. Substitution, cooperative, and solvent effects on π pnicogen bonds in the FH(2)P and FH(2)As complexes.

    An, Xiu-Lin; Li, Ran; Li, Qing-Zhong; Liu, Xiao-Feng; Li, Wen-Zuo; Cheng, Jian-Bo


    Ab initio calculations have been carried out to study the substitution effect on the π pnicogen bond in ZH(2)P-C(2)HM (Z = H, H(3)C, NC, F; M = H, CH(3), Li) dimer, cooperative effect of the π pnicogen bond and hydrogen bond in XH-FH(2)Y-C(2)H(4) (X = HO, NC, F; Y = P and As) trimer, and solvent effect on the π pnicogen bond in FH(2)P-C(2)H(2), FH(2)P-C(2)H(4), FH(2)As-C(2)H(2), and FH(2)As-C(2)H(4) dimers. The interaction energy of π pnicogen bond increases in magnitude from -1.51 kcal mol(-1) in H(3)P-C(2)H(2) dimer to -7.53 kcal mol(-1) in FH(2)P-C(2)HLi dimer at the MP2/aug-cc-pVTZ level. The π pnicogen bond is enhanced by 12-30 % due to the presence of hydrogen bond in the trimer. The π pnicogen bond is also enhanced in solvents. The natural bond orbital analysis and symmetry adapted perturbation theory (SAPT) were used to unveil the source of substitution, cooperative, and solvent effects.

  19. Supporting Personal Semantic Annotations in P2P Semantic Wikis

    Torres, Diego; Skaf-Molli, Hala; Díaz, Alicia; Molli, Pascal

    In this paper, we propose to extend Peer-to-Peer Semantic Wikis with personal semantic annotations. Semantic Wikis are one of the most successful Semantic Web applications. In semantic wikis, wikis pages are annotated with semantic data to facilitate the navigation, information retrieving and ontology emerging. Semantic data represents the shared knowledge base which describes the common understanding of the community. However, in a collaborative knowledge building process the knowledge is basically created by individuals who are involved in a social process. Therefore, it is fundamental to support personal knowledge building in a differentiated way. Currently there are no available semantic wikis that support both personal and shared understandings. In order to overcome this problem, we propose a P2P collaborative knowledge building process and extend semantic wikis with personal annotations facilities to express personal understanding. In this paper, we detail the personal semantic annotation model and show its implementation in P2P semantic wikis. We also detail an evaluation study which shows that personal annotations demand less cognitive efforts than semantic data and are very useful to enrich the shared knowledge base.

  20. Energetics and Dynamics of the Reactions of O(3P) with Dimethyl Methylphosphonate and Saria


    a SN2 -like transition geometry, Figure 3c, the axial O-C-H bond angle is slightly bent more for reaction 4, Figure 3d. The products of reaction 4 are...Energetics and Dynamics of the Reactions of O(3P) with Dimethyl Methylphosphonate and Sarin Patrick F. Conforti and Matthew Braunstein* Spectral...calculations were performed on the reaction systems O(3P) + sarin and O(3P) + dimethyl methylphosphonate (DMMP), a sarin simulant. Transition state

  1. Experimental and theoretical studies of rate coefficients for the reaction O(3P)+CH3OH at high temperatures.

    Lu, Chih-Wei; Chou, Shen-Long; Lee, Yuan-Pern; Xu, Shucheng; Xu, Z F; Lin, M C


    Rate coefficients of the reaction O((3)P) + CH(3)OH in the temperature range of 835-1777 K were determined using a diaphragmless shock tube. O atoms were generated by photolysis of SO(2) with a KrF excimer laser at 248 nm or an ArF excimer laser at 193 nm; their concentrations were monitored via atomic resonance absorption excited by emission from a microwave-discharged mixture of O(2) and He. The rate coefficients determined for the temperature range can be represented by the Arrhenius equation, k(T) = (2.29 +/- 0.18) x 10(-10) exp[-(4210 +/- 100)T] cm(3) molecule(-1) s(-1); unless otherwise noted, all the listed errors represent one standard deviation in fitting. Combination of these and previous data at lower temperature shows a non-Arrhenius behavior described as the three-parameter equation, k(T) = (2.74 +/- 0.07) x 10(-18)T(2.25 +/- 0.13) exp[-(1500 +/- 90)T] cm(3)molecule(-1) s(-1). Theoretical calculations at the Becke-3-Lee-Yang-Parr (B3LYP)6-311 + G(3df,2p) level locate three transition states. Based on the energies computed with coupled clusters singles, doubles (triples) [CCSD(T)]/6-311 + G(3df,2p)B3LYP6-311 + G(3df,2p), the rate coefficients predicted with canonical variational transition state theory with small curvature tunneling corrections agree satisfactorily with the experimental observations. The branching ratios of two accessible reaction channels forming OH + CH(2)OH (1a) and OH + CH(3)O (1b) are predicted to vary strongly with temperature. At 300 K, reaction (1a) dominates, whereas reaction (1b) becomes more important than reaction (1a) above 1700 K.

  2. miR-410-3p suppresses breast cancer progression by targeting Snail.

    Zhang, Ya-Feng; Yu, Yue; Song, Wang-Zhao; Zhang, Rui-Ming; Jin, Shan; Bai, Jun-Wen; Kang, Hong-Bin; Wang, Xin; Cao, Xu-Chen


    miR-410-3p acts as an oncogene or tumor-suppressor gene in various types of cancer. However, its role in breast cancer remains unknown. In the present study, expression of miR-410-3p in 30 breast cancer and paired adjacent normal tissues was detected by RT-qPCR. The expression of miR-410-3p was downregulated in 76.7% of the breast cancer samples. To further validate the expression of miR-410-3p in breast cancer, we analyzed miR-410-3p expression profiling data set from The Cancer Genome Atlas (TCGA) including 683 breast cancer and 87 normal breast tissues. We observed that the expression of miR-410-3p was downregulated in breast cancer tissues. Next, we investigated the influence of miR-410-3p on cell proliferation by transiently transfecting the miR-410-3p mimic or inhibitor, as well as their corresponding controls in the MDA-MB-231 and MCF7 cell lines. miR-410-3p overexpression reduced cell growth, colony formation and the number of EdU-positive cells in the MDA-MB-231 cells. In contrast, inhibition of miR-410-3p in the MCF7 cells resulted in a higher proliferation rate as assessed by MTT assay, plate colony formation and EdU assays. Furthermore, miR-410-3p inhibited epithelial-mesenchymal transition. In addition, Snail was found to be a direct target of miR-410-3p based on a luciferase assay. Overexpression of Snail was able to rescue the effect of miR-410-3p in breast cancer cells. Moreover, miR‑410-3p was inversely expressed with Snail in breast cancer samples. Our data provide new knowledge regarding the role of miR-410-3p in breast cancer progression.

  3. Sizeable beta-strength in 31Ar (beta 3p) decay

    T. Koldste, G.; Blank, B.; J. G. Borge, M.


    We present for the first time precise spectroscopic information on the recently discovered decay mode beta-delayed 3p-emission. The detection of the 3p events gives an increased sensitivity to the high energy part of the Gamow-Teller strength distribution from the decay of 31Ar revealing that as ...

  4. Terminal 3p deletions in two families--correlation between molecular karyotype and phenotype.

    Pohjola, P.; Leeuw, N. de; Penttinen, M.; Kaariainen, H.


    The 3p deletion syndrome is a rare disorder caused by deletions of different sizes in the 3p25-pter region. It is characterized by growth retardation, developmental delay, mental retardation, dysmorphism, microcephaly, and ptosis. The phenotype of individuals with deletions varies from normal to sev

  5. Expression of pRb2/p130 Gene in Human Endometrial Carcinoma Cells and the Significance%子宫内膜癌中pRb2/p130的表达及意义

    支霜; 傅芬


    Objective To investigate the expression of pRb2/p130 gene and its relationship with the patholgical clinical features of human endometrial carcinoma.Methods The levels of pRb2/P130 protein were examined with immunohistochemical SP method in 28 smples of normal endometrial tissue,20 samples of atypical endometrial hyperplasia and 30 samples of endometrial adenocarcinomas.Results The positive rate of pRb2/p130 protein in the normal endometrium,atypical endometrial hyperplasia cells and endometrial adenocarcinoma cells was respectively 82.1%,55.0% and 23.3%.pRb2/p130 protein in endometrial atypical hyperplasia or endometrial adenocarcinoma was significantly lower than that in the normal tissue (P0.05).Conclusion Descent or deletion of the expression of pRb2/p130 gene is of a frequent incidence in human endometrial carcinoma.The positive rate of pRb2/p130 expression is reversly proportionate to malignant degree of endometrial carcinoma,which indicats that pRb2/p130 plays an important role in the development and pathogenesis of endometrial carcinoma.%目的 探讨子宫内膜组织中pRb2/p130的表达及其与子宫内膜癌临床病理特征的关系.方法 应用免疫组化SP法检测pRb2/p130在28例正常增生期子宫内膜、20例不典型增生子宫内膜、30例子宫内膜腺癌组织中的表达.结果 pRb2/p130阳性表达率在正常增生期子宫内膜、不典型增生子宫内膜、子宫内膜腺癌组织中依次递减(分别为82.1%、55.0%、23.3%),且子宫内膜腺癌和不典型性增生中明显低于正常增生期子宫内膜,差异均有统计学意义(P0.05).结论 在子宫内膜癌中pRb2/p130的表达下降或缺失是一个频发事情,其恶性度愈高阳性率愈低,提示pRb2/p130与子宫内膜癌的发生、发展密切相关,并在其发病机制中起着重要的作用.

  6. The direct measurement of the 3 3P0-3 3P1 fine-structure interval and the gJ-factor of atomic silicon by laser magnetic resonance

    Evenson, K. M.; Beltran-Lopez, V.; Ley-Koo, E.; Inguscio, M.


    The J - 1 fine structure interval and the g-factor of the 3P1 state have been determined with high precision in the present laser magnetic resonance measurements of the ground 3p2 3P multiplet of atomic Si. Delta-E(3P1-3P0) = 2,311,755.6(7) MHz, and gJ(3P1) = 1.500830(70). Single-configuration calculations of gJ for 3P1 and 3P2 yield a value for the latter which, at 1.501095, is noted to differ by an unexpectedly large margin from the experimental value.

  7. Peer behavior based proactive P2P worm detection%基于节点行为的主动 P2P 蠕虫检测

    朱晖; 李伟华; 史豪斌


    Proactive P2P worm propagation is a serious security threat to P2P network and Internet. By researching the peer be-havior of propagating proactive P2P worm, this paper puts forward PBD(Peer Behavior based Detection)to detect proactive P2P worm. On this basis, it designs and implements a PPWDS(Proactive P2P Worm Detection System). This system adopts CUSUM algorithm to carry out real time monitoring to the outbound short link of P2P peers. Experiments show that PBD is an effective method of proactive P2P worm detect.%  主动 P2P 蠕虫的传播会对 P2P 网络以及互联网的安全造成严重威胁。通过研究主动 P2P 蠕虫传播时节点行为,提出一种基于节点行为的主动 P2P 蠕虫检测方法 PBD(Peer Behavior based Detection)。在此基础上设计和实现了一个主动 P2P 蠕虫检测系统 PPWDS(Proactive P2P Worm Detection System),该系统采用 CUSUM 算法对 P2P 节点出站短连接进行实时监控。实验表明,PBD 是检测主动 P2P 蠕虫的一种有效方法。

  8. An Effective Calculation of Reputation in P2P Networks

    RVVSV Prasad


    Full Text Available With the advent of sophisticated networking technologies and the related applications, more and more computers are getting hooked to the Internet. This is mainly for utilizing several services ranging from information sharing to electronic transactions. P2P networks which allow decentralized systems, have posed problems related to trust when transactions have to be carried out. Current literature proposes several solutions for trust management and reputation computation. The solutions base their assessment of reputations on the number of successful transactions or on the similarity of the feedbacks. There are some concerns in the feedback ratings if we are not considering the issues like number of transactions, frequency of transactions with the same peer and different peers, age of transaction, how frequently a given peer attends a common vendor, and the number of common vendors between the pairs. This paper puts forward a reputation computation system addressing these concerns. It implicitly allows detection of malicious peers. It also incorporates a corrective mechanism, if the feedbacks are from more number of malicious peers. The implementations and the results that support our claims are also presented.

  9. Survey on Distributed Data Mining in P2P Networks

    T, Rekha Sunny


    The exponential increase of availability of digital data and the necessity to process it in business and scientific fields has literally forced upon us the need to analyze and mine useful knowledge from it. Traditionally data mining has used a data warehousing model of gathering all data into a central site, and then running an algorithm upon that data. Such a centralized approach is fundamentally inappropriate due to many reasons like huge amount of data, infeasibility to centralize data stored at multiple sites, bandwidth limitation and privacy concerns. To solve these problems, Distributed Data Mining (DDM) has emerged as a hot research area. Careful attention in the usage of distributed resources of data, computing, communication, and human factors in a near optimal fashion are paid by distributed data mining. DDM is gaining attention in peer-to-peer (P2P) systems which are emerging as a choice of solution for applications such as file sharing, collaborative movie and song scoring, electronic commerce, an...

  10. Electronic and Rovibrational Quantum Chemical Analysis of C$_3$P$^-$: The Next Interstellar Anion?

    Fortenberry, Ryan C


    C$_3$P$^-$ is analogous to the known interstellar anion C$_3$N$^-$ with phosphorus replacing the nitrogen in a simple step down the periodic table. In this work, it is shown that C$_3$P$^-$ is likely to possess a dipole-bound excited state. It has been hypothesized and observationally supported that dipole-bound excited states are an avenue through which anions could be formed in the interstellar medium. Additionally, C$_3$P$^-$ has a valence excited state that may lead to further stabilization of this molecule, and C$_3$P$^-$ has a larger dipole moment than neutral C$_3$P ($\\sim 6$ D vs. $\\sim 4$ D). As such, C$_3$P$^-$ is probably a more detectable astromolecule than even its corresponding neutral radical. Highly-accurate quantum chemical quartic force fields are also applied to C$_3$P$^-$ and its singly $^{13}$C substituted isotopologues in order to provide structures, vibrational frequencies, and spectroscopic constants that may aid in its detection.

  11. Orientation of Zn3P2 films via phosphidation of Zn precursors

    Katsube, Ryoji; Nose, Yoshitaro


    Orientation of solar absorber is an important factor to achieve high efficiency of thin film solar cells. In the case of Zn3P2 which is a promising absorber of low-cost and high-efficiency solar cells, (110)/(001) orientation was only reported in previous studies. We have successfully prepared (101)-oriented Zn3P2 films by phosphidation of (0001)-oriented Zn films at 350 °C. The phosphidation mechanism of Zn is discussed through STEM observations on the partially-reacted sample and the consideration of the relationship between the crystal structures of Zn and Zn3P2 . We revealed that (0001)-oriented Zn led to nucleation of (101)-oriented Zn3P2 due to the similarity in atomic arrangement between Zn and Zn3P2 . The electrical resistivity of the (101)-oriented Zn3P2 film was lower than those of (110)/(001)-oriented films, which is an advantage of the phosphidation technique to the growth processes in previous works. The results in this study demonstrated that well-conductive Zn3P2 films could be obtained by controlling orientations of crystal grains, and provide a guiding principle for microstructure control in absorber materials.

  12. Evolution of a system sensitive to stochastic noise: P3.p cell fate in Caenorhabditis.

    Pénigault, Jean-Baptiste; Félix, Marie-Anne


    The C. elegans cell lineage is overall invariant. One rare instance of variability concerns P3.p, the most anterior vulva precursor cell, which may either fuse with the epidermis without dividing, or remain competent to form vulval tissue and divide. Here we examine the evolutionary properties of this stochastic variation in P3.p fate. In the Caenorhabditis genus, high P3.p competence is ancestral and reduction in P3.p competence and division frequency occurred in C. sp. 14 and in a clade of nine species. Within this clade, the frequency of P3.p division further varies within and among species, being intermediate in C. elegans and low in C. briggsae. P3.p fate frequency is sensitive to random mutation accumulation, suggesting that this trait may evolve rapidly because of its sensitivity to mutational impact. P3.p fate depends on LIN-39/Hox5 expression and we find that the peak of LIN-39/Hox5 protein level is displaced posteriorly in C. briggsae compared to C. elegans. However, P3.p fate specification is most sensitive to the dose of EGL-20 and CWN-1, two Wnts that are secreted in a long-range gradient from the posterior end of C. elegans larvae (accompanying article). A half-dose of either of these Wnts is sufficient to affect division frequency in C. elegans N2 to levels similar to those in C. briggsae. Symmetrically, we show that an increase in Wnt dose rescues anterior competence in C. briggsae. We propose that evolutionary variation in the concentration or interpretation of the long-range Wnt gradient may be involved in the rapid evolution of P3.p fate in Caenorhabditis.

  13. Sizeable beta-strength in {sup 31}Ar (β3p) decay

    Koldste, G.T. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Blank, B. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Borge, M.J.G.; Briz, J.A.; Carmona-Gallardo, M. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Fraile, L.M. [Grupo de Física Nuclear, Universidad Complutense, CEI Moncloa, E-28040 Madrid (Spain); Fynbo, H.O.U. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Giovinazzo, J. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Johansen, J.G. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Jokinen, A. [Department of Physics, University of Jyväskylä, FIN-40351 Jyväskylä (Finland); Jonson, B. [Fundamental Fysik, Chalmers Tekniska Högskola, S-41296 Göteborg (Sweden); Kurturkian-Nieto, T. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Nilsson, T. [Fundamental Fysik, Chalmers Tekniska Högskola, S-41296 Göteborg (Sweden); Perea, A.; Pesudo, V. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); and others


    We present for the first time precise spectroscopic information on the recently discovered decay mode β-delayed 3p-emission. The detection of the 3p events gives an increased sensitivity to the high energy part of the Gamow–Teller strength distribution from the decay of {sup 31}Ar revealing that as much as 30% of the strength resides in the β3p-decay mode. A simplified description of how the main decay modes evolve as the excitation energy increases in {sup 31}Cl is provided.

  14. Progress on the Multiphysics Capabilities of the Parallel Electromagnetic ACE3P Simulation Suite

    Kononenko, Oleksiy [SLAC National Accelerator Lab., Menlo Park, CA (United States)


    ACE3P is a 3D parallel simulation suite that is being developed at SLAC National Accelerator Laboratory. Effectively utilizing supercomputer resources, ACE3P has become a key tool for the coupled electromagnetic, thermal and mechanical research and design of particle accelerators. Based on the existing finite-element infrastructure, a massively parallel eigensolver is developed for modal analysis of mechanical structures. It complements a set of the multiphysics tools in ACE3P and, in particular, can be used for the comprehensive study of microphonics in accelerating cavities ensuring the operational reliability of a particle accelerator.

  15. Elevation of circulating miR-210-3p in high-altitude hypoxic environment

    Yan eYan


    Full Text Available Background: The induction of miR-210-3p, a master hypoxamir, is a consistent feature of the hypoxic response in both normal and malignant cells. However, whether miR-210-3p acts as a circulating factor in response to a hypoxic environment remains unknown. The current study aimed to examine the effect of a high-altitude hypoxic environment on circulating miR-210-3p.Methods: We examined and compared the levels of miR-210-3p using TaqMan-based qRT-PCR in both peripheral blood cells and plasma from 84 ethnic Chinese Tibetans residing at 3560 m, 46 newly arrived migrant Han Chinese (Tibet Han and 82 Han Chinese residing at 8.9 m (Nanjing Han. Furthermore, we analyzed the correlations of miR-210-3p with hematological indices. Results: The relative concentrations of miR-210-3p to internal reference U6 in blood cells were significantly higher in the Tibet Han group (1.01±0.11, P<0.001 and in the Tibetan group (1.17±0.09, P<0.001 than in the Nanjing Han group (0.51±0.04. The absolute concentrations of plasma miR-210-3p were also markedly elevated in the Tibet Han group (503.54±42.95 fmol/L, P=0.004 and in the Tibetan group (557.78±39.84 fmol/L, P<0.001 compared to the Nanjing Han group (358.39±16.16 fmol/L. However, in both blood cells and plasma, miR-210-3p levels were not significantly different between the Tibet Han group and the Tibetan group (P=0.280, P=0.620, respectively. Plasma miR-210-3p concentrations were positively correlated with miR-210-3p levels in blood cells (r=0.192, P=0.005. Furthermore, miR-210-3p levels in both blood cells and plasma showed strong positive correlations with red blood cell counts and hemoglobin and hematocrit values. Conclusion: These data demonstrated, for the first time, that miR-210-3p might act as a circulating factor in response to hypoxic environments and could be associated with human adaptation to life at high altitudes.

  16. 多通道反应O(3P)+CH2F的理论研究%Vibrational Mode Analysis for the Multi-channel Reactions of O(3P) with CH2F

    曹小龙; 郭丽


    用密度泛函理论研究了氧原子与氟代甲基自由基的反应.反应中出现的所有物种的平衡构型用B3LYP方法在6-311++G(2 d,2p)基组水平上进行了优化,同时对各物种进行了频率分析;在同一理论水平上计算了各反应通道的势能面变化,分析了反应物、中间体、过渡态、产物的振动模式随反应途径的变化关系,阐明了该多通道反应的反应机理.%All species involved in the multi-channel reaction of an oxygen atom with a fluorinated methyl radical have been investigated using density functional theory (DFT) . The geometries of the reactants, intermediates, transition states and products are optimized at the B3LYP/6-311 + + G(2 d, 2p) level. The potential energy surface for this reaction is calculated at the same level of theory. Various possible production channels involved in the reaction of O(3 P) +CH2F are examined. The vibrational mode analysis is used to elucidate the relationship between the transition states, intermediates and the products. The extensive investigation shows that the reaction mechanism is reliable.

  17. Isotope shifts of the (3s3p) 3P0,1,2-(3s4s) 3S1 Mg I transitions

    He, Ming; Therkildsen, Kasper T.; Jensen, Brian B.;


    We report measurements of the isotope shifts of the (3s3p)P30,1,2-(3s4s)S31 Mg I transitions for the stable isotopes M24g (I=0) , M25g (I=5/2) , and M26g (I=0) . Furthermore, the M25g S31 hyperfine coefficient A(S31)=(-321.6±1.5)MHz is extracted and found to be in excellent agreement with state...

  18. The Application of 3P+MYSQL Technology of Dynamic Website Design%3P+MYSQL技术在动态网站设计中的运用

    周悦; 朱小社


    在Windows2000 Server平台下分别利用ASP、PHP、JSP这三个动态网站技术,使用可以跨Windows和Linux平台的MYSQL数据库,采用三种连接数据库的方法,在三种不同特点的WEB服务器环境下,给出了动态网站设计中3P+MYSQL技术的运用.

  19. Specific production of very long-lived core-excited sulfur atoms by 2p{sup -1}{sigma}* excitation of the OCS molecule followed by ultrafast dissociation

    Fink, Reinhold F [Department of Physics, Uppsala University, Box 530, SE-751 21 Uppsala (Sweden); Eschner, Annika [Department of Synchrotron Radiation Research, Institute of Physics, University of Lund, Box 118, SE-221 00 Lund (Sweden); Magnuson, Martin [Department of Physics, Uppsala University, Box 530, SE-751 21 Uppsala (Sweden); Bjoerneholm, Olle [Department of Physics, Uppsala University, Box 530, SE-751 21 Uppsala (Sweden); Hjelte, Ingela [Department of Physics, Uppsala University, Box 530, SE-751 21 Uppsala (Sweden); Miron, Catalin [MAXLAB, University of Lund, Box 118, SE-221 00 Lund (Sweden); Bassler, Margit [MAXLAB, University of Lund, Box 118, SE-221 00 Lund (Sweden); Svensson, Svante [Department of Physics, Uppsala University, Box 530, SE-751 21 Uppsala (Sweden); Piancastelli, Maria Novella [Department of Physics, Uppsala University, Box 530, SE-751 21 Uppsala (Sweden); Sorensen, Stacey L [Department of Synchrotron Radiation Research, Institute of Physics, University of Lund, Box 118, SE-221 00 Lund (Sweden)


    A core-excited sulfur state with a lifetime almost one order of magnitude longer than in molecular 2p core-hole states is selectively produced by ultrafast dissociation of S 2p {yields} {sigma}* excited OCS. Clear evidence for this is provided by strong atomic peaks (20% of the total intensity) in x-ray fluorescence but very weak ones (2%) in the corresponding resonant Auger spectrum. Corroborating the assignment of the spectra, ab initio calculations explain the enhanced lifetime: the Auger decay of the produced {sup 3}D{sub 3} (2p{sup 5}3p{sup 5}) sulfur state is strongly decreased as it contradicts a newly derived propensity rule of the L{sub 2,3}MM Auger decay. (letter to the editor)

  20. Observation of the 2p3/2 -> 2s1/2 intra-shell transition in He-like uranium

    Trassinelli, Martino; Beyer, Heinrich; Indelicato, Paul; Märtin, Renate; Reuschl, Regina; Kozhedub, Yuri S; Brandau, Carsten; Bräuning, Harald; Geyer, Sabrina; Gumberidze, Alexander; Hess, Sebastian; Jagodzinski, Pawel; Kozhuharov, Christophor; Liesen, Dieter; Trotsenko, Sergiy; Weber, Günter; Winters, Danyal; Stöhlker, Thomas


    We present the first identification and energy measurement of the 1s2p 3P2 \\to 1s2s 3S1 transition in He-like uranium. The experiment was performed at the internal gas-jet target of the ESR storage ring at GSI by means of a Bragg crystal spectrometer. Using the 1s2 2p 2P3/2 \\to 1s2 2s 2S1/2 transition in Li-like uranium as reference, we obtained an accuracy in the order of 0.01%, which provides, at present, the most accurate test of many-body and QED contributions in very heavy He-like ions

  1. Bonding and Site Preferences in the New Quasi-Binary Zr 2.7Hf 11.3P 9

    Kleinke, Holger; Franzen, Hugo F.


    The new quasi-binary Zr 2.7Hf 11.3P 9was synthesized by arc-melting of Zr, Hf, Co, and HfP in a ratio corresponding to the initial composition "Zr 2.25Hf 6.75Co 2P 4". Zr 2.7Hf 11.3P 9crystallizes in the Zr 14P 9structure type, which is unknown in the binary Hf/P system. The ideal orthorhombic lattice dimensions (space group Pnnm(No. 58), Z=4) were refined to a=16.640(7) Å, b=27.40(2) Å, c=3.619(1) Å, V=1650(2) Å 3. The structure consists of three-dimensional condensed one-, two-, and three-capped trigonal (Zr, Hf) 6P prisms, occurring with numerous short M- Mbonds ( M=Zr, Hf). Each of the 15 metal sites is statistically occupied by a mixture of Zr and Hf, which varies significantly from site to site. The Hf/Zr ratio in a given site depends on the M- Mand M-P interactions. The systematic increase of this ratio with increasing total bond order, as evaluated via Mulliken overlap populations and Pauling bond orders, can be understood based on the trend that Hf forms stronger M- Mand M-P bonds than Zr. As expected for a metal-rich phosphide, band structure calculations for the hypothetical "Hf 14P 9" carried out with the extended Hückel approximation result in a significant density of states at the Fermi level.

  2. Surface and interface properties of Zn3P2 solar cells

    Kazmerski, L. L.; Ireland, P. J.; Catalano, A.


    The compositional properties of Zn3P2 thin films are studied using surface analysis techniques. Relative sensitivity factors of S(Zn sub LMM) = 0.29 and S(P sub LMM) = 0.34 are determined for this semiconductor with the aid of Auger electron spectroscopy standard spectra. The interfacial properties of the Mg/Zn3P2 thin-film solar cell are studied using Auger electron spectroscopy depth-compositional profiling in conjunction with complementary secondary ion mass spectroscopy and X-ray photoelectron spectroscopy data. Evidence is adduced for the formation of Mg3P2 at the metal-semiconductor interface. It is noted that this compound has a bandgap near that of Zn3P2. Attention is given to the possible formation of a heterojunction as a result of the interfacial reaction.

  3. Genetic epidemiology of Paget's disease of bone in italy: sequestosome1/p62 gene mutational test and haplotype analysis at 5q35 in a large representative series of sporadic and familial Italian cases of Paget's disease of bone.

    Falchetti, Alberto; Di Stefano, Marco; Marini, Francesca; Ortolani, Sergio; Ulivieri, Massimo Fabio; Bergui, Simona; Masi, Laura; Cepollaro, Chiara; Benucci, Maurizio; Di Munno, Ombretta; Rossini, Maurizio; Adami, Silvano; Del Puente, Antonio; Isaia, Giancarlo; Torricelli, Francesca; Brandi, Maria Luisa


    Families affected by Paget's disease of bone frequently harbor mutations in the SQSTM1/p62 gene. In this multicentric study we collected 345 sporadic and 12 familial PDB cases throughout Italy, identifying 12 different mutations, 5 of which are newly reported and 3, D335E, A381V, and Y383X, external to the UBA domain. Subjects with truncating mutations, E396X, showed a significantly younger age at clinical diagnosis, while the Y383X subjects had a higher average number of affected skeletal sites. All the mutants exhibited the CGTG-H2 haplotype. In two pairs and one triad of unrelated Italian PDB families from different Italian regions, we detected a common SQSTM1/p62 mutation for each P392L, M404V, and G425R group. Since the CGTG-H2 haplotype frequency was also high in normal subjects, and genetic influence due to migratory fluxes of different ethnic groups exists in the Italian population, to refine the search for a more geographically specific founder effect, we extended the haplotype analysis in these families using polymorphic microsatellite repeat markers, within and flanking the SQSTM1/p62 locus, from chromosome 5q35, other than the exon 6 and 3'UTR polymorphisms. All mutant carriers from two of the three M404V families and from the G425R families exhibited common extended chromosome 5q35 haplotypes, IT01 and IT02, respectively, which may be reflecting influences of past migrations. This may be helpful in estimating the true rate of de novo mutations. We confirm the data on the existence of both a mutational hotspot at the UBA domain of SQSTM1/p62 and a founder effect in the PDB population.

  4. Photoionization cross sections of the excited 3s3p 3Po state for atomic Mg

    Wang, Guoli; Wan, Jianjie; Zhou, Xiaoxin


    The photoionization cross sections of the excited levels (3s3p 0,1,2,o 3P) of atomic Mg have been studied theoretically using both the nonrelativistic and fully relativistic R-matrix method. For the threshold cross sections, as previous nonrelativistic studies, present calculations show significant differences (a factor of 3) from former experimental values. Large discrepancies with experiment calls for additional measurements of the photoionization cross sections from the excited states of Mg.

  5. Determination of diffusion, reflection and deexcitation coefficients of metastable excited Ne(3P2) atom

    Suzuki, S.; Itoh, H.


    The diffusion coefficient of the metastable excited Ne(3P2) atom in neon, the reflection coefficient of Ne(3P2) at the surface of an electrode and the rate coefficient of Ne(3P2) for collisional quenching by Ne(1S0) were determined from the gas pressure dependence of the effective lifetime of Ne(3P2). The effective lifetime of Ne(3P2) was measured from the transient current after turning off the Ultraviolet (UV) light in a Townsend discharge. The observed transient current waveform was analysed by solving the diffusion equation for the metastable excited Ne(3P2) atom using the third kind of boundary condition. The rate coefficient of Ne(3P2) for collisional quenching by Ne(1S0) and the reflection coefficient were determined by a nonspectroscopic method for the first time in neon to the best of our knowledge and were (3.2  ±  0.4)  ×  10-16 cm3 s-1 and 0.10  ±  0.04, respectively. The obtained diffusion coefficient at 1 Torr was 177  ±  17 cm2 s-1, which is consistent with the value reported by Dixon and Grant. Moreover, the present results are compared with the results of Phelps and were found to be in good agreement. We also discuss the deexcitation rate of Ne(3P2) at pressures of up to 60 Torr in comparison with previously reported values.

  6. Misregulation of Scm3p/HJURP Causes Chromosome Instability in Saccharomyces cerevisiae and Human Cells

    Prashant K. Mishra; Wei-Chun Au; John S. Choy; P Henning Kuich; Baker, Richard E.; Foltz, Daniel R.; Basrai, Munira A.


    The kinetochore (centromeric DNA and associated proteins) is a key determinant for high fidelity chromosome transmission. Evolutionarily conserved Scm3p is an essential component of centromeric chromatin and is required for assembly and function of kinetochores in humans, fission yeast, and budding yeast. Overexpression of HJURP, the mammalian homolog of budding yeast Scm3p, has been observed in lung and breast cancers and is associated with poor prognosis; however, the physiological relevanc...

  7. Cu3P/RGO Nanocomposite as a New Anode for Lithium-Ion Batteries

    Liu, Shuling; He, Xiaodong; Zhu, Jianping; Xu, Liqiang; Tong, Jianbo


    Cu3P/reduced graphene oxide (Cu3P/RGO) nanocomposite was successfully synthesized by a facile one-pot method as an advanced anode material for high-performance lithium-ion batteries. Cu3P nanostructures with a polyhedral shape with the mean diameter (80–100 nm) were homogeneously anchored on the surface of RGO. The flexible RGO sheets acted as elastic buffering layer which not only reduced the volume change, but also prevented the aggregation of Cu3P nanostructures, the cracking and crumbing of electrodes. On the other hand, the presence of Cu3P nanostructures could also avoid the agglomeration of RGO sheets and retain their highly active surface area. Therefore, as an advanced anode material for high-performance lithium-ion batteries, the as-prepared Cu3P/RGO exhibited high capacity of 756.15 mAhg‑1 at the current density 500 mAg‑1 after 80 cycles, superior cyclic stability and good rate capability.

  8. Effect of Fe2P in LiFePO4/Fe2P composite on electrochemical properties synthesized by MA and control of heat condition

    PARK Jong Suk; LEE Kyung Tae; LEE Kyung Sub


    In order to control the size and distribution of the high conductive Fe2P in LiFePO4/Fe2P composite, two different cooling rates (Fast: 15 ℃·min-1, Slow: 2 ℃·min-1) were employed after mechanical alloying.The discharge capacity of the fast cooled was 83 mAh·g-1 and the slow cooled 121 mAh·g-1.The particle size of the synthesized powder was examined by transmission electron microscopy and distribution of Fe2P was characterized using scanning electron microscopy (SEM).In addition, two-step heat treatment was carried out for better distribution of Fe2P.X-ray diffraction (XRD) and Rietveld refinement reveal that LiFePO4/Fe2P composite consists of 95.77% LiFePO4 and 4.33% of Fe2P.

  9. Non-P2P network applicationautomatic feature recognition%非 P2P 网络应用自动化特征提取

    丁斌; 焦素云; 闫晓亮


    在对流量进行分类的基础上,应用Apriori算法分析分类以后的非P2P流,能够更准确地分析出非P2 P的网络应用特征。%Based on the classified traffic flow between P2P and non‐P2P ,Apriori algorithm is used to analyzing the non‐P2P flow to get the high precise characteristics of non‐P2P network application .

  10. Microduplications of 3p26.3p26.2 containing CRBN gene in patients with intellectual disability and behavior abnormalities.

    Papuc, Sorina M; Hackmann, Karl; Andrieux, Joris; Vincent-Delorme, Catherine; Budişteanu, Magdalena; Arghir, Aurora; Schrock, Evelin; Ţuţulan-Cuniţă, Andreea C; Di Donato, Nataliya


    We report on the clinical data and molecular cytogenetic findings in three unrelated patients presenting with intellectual disability and behavior abnormalities. An overlapping microduplication involving 3p26.2-26.3 was identified in these patients. All three aberrations were confirmed and proven to be parentally inherited. The sizes of the duplications were different, with a common minimal region of 423,754 bp containing two genes - TRNT1 and CRBN. Here, we hypothesize that the copy number gain of CRBN gene might be responsible for developmental delay/intellectual disability.

  11. Genistein up-regulates tumor suppressor microRNA-574-3p in prostate cancer.

    Takeshi Chiyomaru

    Full Text Available Genistein has been shown to inhibit cancers both in vitro and in vivo, by altering the expression of several microRNAs (miRNAs. In this study, we focused on tumor suppressor miRNAs regulated by genistein and investigated their function in prostate cancer (PCa and target pathways. Using miRNA microarray analysis and real-time RT-PCR we observed that miR-574-3p was significantly up-regulated in PCa cells treated with genistein compared with vehicle control. The expression of miR-574-3p was significantly lower in PCa cell lines and clinical PCa tissues compared with normal prostate cells (RWPE-1 and adjacent normal tissues. Low expression level of miR-574-3p was correlated with advanced tumor stage and higher Gleason score in PCa specimens. Re-expression of miR-574-3p in PCa cells significantly inhibited cell proliferation, migration and invasion in vitro and in vivo. miR-574-3p restoration induced apoptosis through reducing Bcl-xL and activating caspase-9 and caspase-3. Using GeneCodis software analysis, several pathways affected by miR-574-3p were identified, such as 'Pathways in cancer', 'Jak-STAT signaling pathway', and 'Wnt signaling pathway'. Luciferase reporter assays demonstrated that miR-574-3p directly binds to the 3' UTR of several target genes (such as RAC1, EGFR and EP300 that are components of 'Pathways in cancer'. Quantitative real-time PCR and Western analysis showed that the mRNA and protein expression levels of the three target genes in PCa cells were markedly down-regulated with miR-574-3p. Loss-of-function studies demonstrated that the three target genes significantly affect cell proliferation, migration and invasion in PCa cell lines. Our results show that genistein up-regulates tumor suppressor miR-574-3p expression targeting several cell signaling pathways. These findings enhance understanding of how genistein regulates with miRNA in PCa.

  12. Phase equilibria in the Mo-Fe-P system at 800 °C and structure of ternary phosphide (Mo(1-x)Fe(x))3P (0.10 ≤ x ≤ 0.15).

    Oliynyk, Anton O; Lomnytska, Yaroslava F; Dzevenko, Mariya V; Stoyko, Stanislav S; Mar, Arthur


    Construction of the isothermal section in the metal-rich portion (<67 atom % P) of the Mo-Fe-P phase diagram at 800 °C has led to the identification of two new ternary phases: (Mo(1-x)Fe(x))(2)P (x = 0.30-0.82) and (Mo(1-x)Fe(x))(3)P (x = 0.10-0.15). The occurrence of a Co(2)Si-type ternary phase (Mo(1-x)Fe(x))(2)P, which straddles the equiatomic composition MoFeP, is common to other ternary transition-metal phosphide systems. However, the ternary phase (Mo(1-x)Fe(x))(3)P is unusual because it is distinct from the binary phase Mo(3)P, notwithstanding their similar compositions and structures. The relationship has been clarified through single-crystal X-ray diffraction studies on Mo(3)P (α-V(3)S-type, space group I42m, a = 9.7925(11) Å, c = 4.8246(6) Å) and (Mo(0.85)Fe(0.15))(3)P (Ni(3)P-type, space group I4, a = 9.6982(8) Å, c = 4.7590(4) Å) at -100 °C. Representation in terms of nets containing fused triangles provides a pathway to transform these closely related structures through twisting. Band structure calculations support the adoption of these structure types and the site preference of Fe atoms. Electrical resistivity measurements on (Mo(0.85)Fe(0.15))(3)P reveal metallic behavior but no superconducting transition.

  13. Magnetic Properties of Quantized Vortices in Neutron $^3P_2$ Superfluids in Neutron Stars

    Masuda, Kota


    We discuss quantized vortices in neutron $^3P_2$ superfluids, which are believed to realize in high density neutron matter such as neutron stars. By using the Ginzburg-Landau free energy for $^3P_2$ superfluids, we determine the ground state in the absence and presence of the external magnetic field, and numerically construct $^3P_2$ quantized vortices in the absence and presence of the external magnetic field along the vortex axis (poloidal) or angular direction (toroidal). We find in certain situations the spontaneous magnetization of the vortex core, whose typical magnitude is about $10^{7-8}$ Gauss, but the net magnetic field in a neutron star is negligible because of the ratio of the vortex core size $\\sim 10$fm and the intervortex distance $\\sim 10^{-6}$m in a vortex lattice.

  14. Experimental and theoretical study of 3p photoionization and subsequent Auger decay in atomic chromium

    Keskinen, J.; Huttula, S.-M.; Mäkinen, A.; Patanen, M.; Huttula, M.


    3p photoionization and subsequent low kinetic energy Coster-Kronig and super Coster-Kronig Auger decay have been studied in atomic chromium. The binding energies, line widths, and relative intensities for the transitions seen in the synchrotron radiation excited 3p photoelectron spectrum are determined. The high resolution M2,3 M4,5 M4,5 and M2,3 M4,5 N1 Auger electron spectra following the electron impact excited 3p ionization are presented and the kinetic energies, relative intensities, and identifications are given for the main lines. The experimental findings are compared with the theoretical predictions obtained from Hartree-Fock and multiconfiguration Dirac-Fock approaches.

  15. A new form of Ca3P2 with a ring of Dirac nodes

    Lilia S. Xie


    Full Text Available We report the synthesis and crystal structure of a new high-temperature form of Ca3P2. The crystal structure was determined through Rietveld refinements of synchrotron powder x-ray diffraction data. This form of Ca3P2 has a crystal structure of the hexagonal Mn5Si3 type, with a Ca ion deficiency compared to the ideal 5:3 stoichiometry. This yields a stable, charge-balanced compound of Ca2+ and P3−. We also report the observation of a secondary hydride phase, Ca5P3H, which again is a charge-balanced compound. The calculated band structure of Ca3P2 indicates that it is a three-dimensional Dirac semimetal with a highly unusual ring of Dirac nodes at the Fermi level. The Dirac states are protected against gap opening by a mirror plane in a manner analogous to what is seen for graphene.

  16. BOREAS Level-3p Landsat TM Imagery: Geocoded and Scaled At-sensor Radiance

    Nickeson, Jaime; Knapp, David; Newcomer, Jeffrey A.; Hall, Forrest G. (Editor); Cihlar, Josef


    For BOReal Ecosystem-Atmosphere Study (BOREAS), the level-3p Landsat Thematic Mapper (TM) data were used to supplement the level-3s Landsat TM products. Along with the other remotely sensed images, the Landsat TM images were collected in order to provide spatially extensive information over the primary study areas. This information includes radiant energy, detailed land cover, and biophysical parameter maps such as Fraction of Photosynthetically Active Radiation (FPAR) and Leaf Area Index (LAI). Although very similar to the level-3s Landsat TM products, the level-3p images were processed with ground control information, which improved the accuracy of the geographic coordinates provided. Geographically, the level-3p images cover the BOREAS Northern Study Area (NSA) and Southern Study Area (SSA). Temporally, the four images cover the period of 20-Aug-1988 to 07-Jun-1994. Except for the 07-Jun-1994 image, which contains seven bands, the other three contain only three bands.

  17. 3p14 De Novo Interstitial Microdeletion in a Patient with Intellectual Disability and Autistic Features with Language Impairment: A Comparison with Similar Cases

    Ana Belén de la Hoz


    Full Text Available To date, few cases of 3p proximal interstitial deletions have been reported and the phenotype and genotype correlation is not well understood. Here, we report a new case of a 3p proximal interstitial deletion. The patient is an 11-year-old female with speech and social interaction difficulties, learning disability, and slight facial dysmorphism, but no other major malformations. An 8 Mb de novo interstitial deletion at 3p14.2-p14.1, from position 60.461.316 to 68.515.453, was revealed by means of array comparative genomic hybridization and confirmed using quantitative reverse-transcription polymerase chain reaction assays. This region includes six genes: FEZF2, CADPS, SYNPR, ATXN7, PRICKLE, and MAGI1, that are known to have a role in neurodevelopment. These genes are located on the proximal side of the deletion. We compare our case with previously well-defined patients reported in the literature and databases.

  18. Domain architectures of the Scm3p protein provide insights into centromere function and evolution

    Aravind, L.; Lakshminarayan, M. Iyer; Wu, Carl


    Recently, Scm3p has been shown to be a nonhistone component of centromeric chromatin that binds stoichiometrically to CenH3–H4 histones, and to be required for the assembly of kinetochores in S. cerevisiae. Scm3p is conserved across fungi, and displays a remarkable variation in protein size, ranging from ~200 amino acids in Saccharomyces cerevisiae to ~1300 amino acids in Neurospora crassa. This is primarily due a variable C-terminal segment that is linked to a conserved N-terminal, CenH3-int...

  19. Autism spectrum disorder, Klinefelter syndrome, and chromosome 3p21.31 duplication: a case report.

    Stuart, Scott W; King, Casey H; Pai, G Shashidar


    Autism spectrum disorders are heterogeneous in nature with idiopathic and genetic origins. We present a 7-year-old boy with a long history of multiple behavioral concerns, poor school performance, repetitive/compulsive tendencies, poor social skills, and language delays. A multidisciplinary evaluation concluded that the patient met full criteria for autism. A genetic evaluation demonstrated Klinefelter syndrome 47, XXY karyotype with concurrent duplication of 3p21.31 by microarray analysis. Maternal genetic analysis demonstrated the same 3p21.31 duplication. The potential implication with regard to autism spectrum disorders has not been previously discussed in the literature.

  20. Data of evolutionary structure change: 1Q95E-2P2GD [Confc[Archive


  1. Observation of the Forbidden Magnetic Dipole Transition 6{sup 2}P{sub ½} --> 7{sup 2}P{sub ½} in Atomic Thallium

    Chu, S.


    A measurement of the 6{sup 2}P{sub ½} --> 7{sup 2}P{sub ½} forbidden magnetic dipole matrix element in atomic thallium is described. A pulsed, linearly polarized dye laser tuned to the transition frequency is used to excite the thallium vapor from the 6{sup 2}P{sub ½} ground state to the 7{sup 2}P{sub ½} excited state. Interference between the magnetic dipole M1 amplitude and a static electric field induced E1 amplitude results in an atomic polarization of the 7{sup 2}P{sub ½} state, and the subsequent circular polarization of 535 nm fluorescence. The circular polarization is seen to be proportional to / as expected, and measured for several transitions between hyperfine levels of the 6{sup 2}P{sub ½} and 7{sup 2}P{sub ½} states. The result is = -(2.11 +- 0.30) x 10{sup -5} parallel bar e parallel bar dirac constant/2mc, in agreement with theory.

  2. Multi-configuration Dirac-Hartree-Fock calculations of the transition rates of 2s22p2 - 2s2p3 and 2s2p3 - 2s22pnl (n ≥ 3) E1 transitions of N+

    Shen, Xiaozhi; Liu, Juan; Zhou, Fuyang


    Wavefunctions were determined using the multi-configuration Dirac-Hartree-Fock method. The core-core, core-valence, valence correlation, Breit interaction and quantum electrodynamics effects, as well as some higher-order correlation effects, were considered to obtain accurate wavelengths (λ), oscillator strengths (gf) and transition rates (A) of 2s22p2 - 2s2p3, 2s2p3 - 2s22pnl (n ≥ 3) and 2s2p3 - 2s2p23s E1 transitions. The branching ratio of 2s2p3 5S^o_2 (namely Aλ2143.45/Aλ2139.68) based on the latest calculation of 2.462 ± 0.119 is recommended for the determination of a nebula's electron temperature and electron density. The largest calculated gf value of 2s2p3 - 2s22p4p is λ630.65, differing from that of λ1060.2 (i.e. 2s2p3 3P^o_2 - 2s22p4p 3S1) that was observed with the largest intensities in the Orion Nebula spectrum. In addition, the energy levels and the splittings of 2s2p3, the extremely difficult calculations of the rates of two-electron one-photon transitions as well as those of the very small intercombination A of 2s2p3 5S^o_2 were studied in detail. Because of the weak spin-orbit interaction, accurately calculating the levels 3P^o_{1,2,0} (or 3D^o_{3,2,1}) and their transition matrix elements is very sensitive to relativistic and electron correlation effects. A special case for this is when the transition operators synchronously applied to wavefunctions with regard to 2s2p3 3Po and 2s22pnl (n = 4) become extremely sensitive to some higher-order correlation effects.

  3. The infinite order point on Y~2=X~3-p~3



    The parameterization of the elliptic curve Y2=X2+1 is given by using the modular forms of X(12) Then using class field theory over imaginary quadratic field and Shimura reciprocity, an infinite order point on the curve y2- = x3-p3 is constructed, for prime p=7 (mod 24).

  4. miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation

    Qiu, Weimin; Kassem, Moustapha


    Wnt signaling determines human stromal (mesenchymal) stem cell (hMSC) differentiation fate into the osteoblast or adipocyte lineage. microRNAs (miRNAs) are small RNA molecules of 21-25 nucleotides that regulate many aspects of osteoblast biology. Thus, we examined miRNAs regulated by Wnt signaling...... in hMSC. We identified miRNA (miR)-141-3p as a Wnt target which in turn inhibited Wnt signaling. Moreover, miR-141-3p inhibited hMSC proliferation by arresting cells at the G1 phase of the cell cycle. miR-141-3p inhibited osteoblast differentiation of hMSC as evidenced by reduced alkaline phosphatase...... activity, gene expression and in vitro mineralized matrix formation. Bioinformatic studies, Western blot analysis and 3'UTR reporter assay demonstrated that cell division cycle 25A (CDC25A) is a direct target of miR-141-3p. siRNA-mediated knock-down of CDC25A inhibited hMSC proliferation and osteoblast...

  5. Construction of a consistent YAC contig for human chromosome region 3p14.1

    Bardenheuer, W; Michaelis, S; Lux, A; Vieten, L; Brocker, F; Julicher, K; Willers, C; Siebert, R; Smith, DI; vanderHout, AH; Buys, C; Schutte, J; Opalka, B


    Chromosomal deletions and translocations of human chromosome region 3p14 are observed in various human malignancies and suggest the existence of a tumor suppressor gene locus within this region. Tumors most frequently affected by these aberrations are small-cell lung cancer and renal-cell carcinoma.

  6. Baryonic {sup 3}P{sub 2} superfluidity under charged-pion condensation with {delta} isobar

    Takatsuka, T.; Tamagaki, R. [Iwate Univ., Morioka, Iwate (Japan)


    We study the baryonic {sup 3}P{sup 2} superfluidity under charged-pion condensation with isobar ({delta}) degrees of freedom. After a remark on motivations of the present study, the outline of theoretical framework is briefly described, typical results of the superfluid critical temperature are shown, and the possibility of coexistence of the superfluid with charged-pion condensation is discussed. (author)

  7. Using P3P in a web services-based context-aware application platform

    Zuidweg, M.; Goncalves Filho, J.; Sinderen, van M.J.; Halasz, E.; Lukovszki, C.; Marosits, T.


    This paper describes a proposal for a privacy control architecture to be applied in the WASP project. The WASP project aims to develop a context-aware service platform on top of 3G networks, using web services technology. The proposed privacy control architecture is based on the P3P privacy policy d

  8. Repumping of ultracold strontium atoms using the ^3P2 - ^3D2 transition

    Mickelson, P. G.; Martinez de Escobar, Y. N.; Traverso, A. J.; Killian, T. C.


    We discuss recent experiments involving ultracold strontium. Using a commercially-available 3 micron laser, we repump atoms out of the ^3P2 level via the ^3D2 state and gain almost a factor of 10 in the number of atoms in our system. This increase in the signal-to-noise ratio enables improved spectroscopy of strontium in our optical trap.

  9. Evidence for locus heterogeneity in acrocephalosyndactyly: A refined localization for the Saethre-Chotzen syndrome locus on distal chromosome 7p-and exclusion of Jackson-Weiss syndrome from craniosynostosis loci on 7p and 5q

    Herwerden, L. van; Rose, C.S.P.; Reardon, W.; Malcolm, S.; Winter, R.M. (Institute of Child Health, London (United Kingdom)); Brueton, L.A. (Northwick Park Hospital, Harrow (United Kingdom)); Weissenbach, J. (Human Genome Research Centre, Evry (France))


    Craniosynostosis (premature fusion of the skull sutures) occurs as a clinically heterogeneous group of disorders, frequently involving digital abnormalities. The authors have previously provisionally assigned the gene for one such condition, Saethre-Chotzen syndrome (ACS III), to chromosome 7p. Linkage analysis is now reported between ACS III and dinucleotide repeat loci on distal 7p. The maximum lod scores, Z[sub max], were 5.57 at a recombination fraction of .05, with D7S488, and 4.74 at a recombination fraction of .05, with D7S493. Only weak linkage, not reaching significance, was found with distal markers (D7S513 and afm281vc9) and a proximal marker (D7S516). Multipoint analysis shows that the disease locus lies between D7S513 and D7S516. Analysis of individual recombinants shows that the most likely position is between D7S493 and D7S516. Linkage data in regard of Jackson-Weiss syndrome demonstrate that this autosomal dominant form of acrocephalosyndactyly does not map to the ACS III region on 7p or to the acrocephalosyndactyly locus on 5q (Boston type). These findings underline the genetic heterogeneity among the different clinical conditions manifesting with acrocephalosyndactyly. 20 refs., 3 figs., 2 tabs.

  10. Evidence for locus heterogeneity in acrocephalosyndactyly: a refined localization for the Saethre-Chotzen syndrome locus on distal chromosome 7p--and exclusion of Jackson-Weiss syndrome from craniosynostosis loci on 7p and 5q.

    van Herwerden, L; Rose, C S; Reardon, W; Brueton, L A; Weissenbach, J; Malcolm, S; Winter, R M


    Craniosynostosis (premature fusion of the skull sutures) occurs as a clinically heterogeneous group of disorders, frequently involving digital abnormalities. We have previously provisionally assigned the gene for one such condition, Saethre-Chotzen syndrome (ACS III), to chromosome 7p. Linkage analysis is now reported between ACS III and dinucleotide repeat loci on distal 7p. The maximum lod scores, Zmax, were 5.57 at a recombination fraction of .05, with D7S488, and 4.74 at a recombination fraction of .05, with D7S493. Only weak linkage, not reaching significance, was found with distal markers (D7S513 and afm281vc9) and a proximal marker (D7S516). Multipoint analysis shows that the disease locus lies between D7S513 and D7S516. Analysis of individual recombinants shows that the most likely position is between D7S493 and D7S516. Linkage data in regard of Jackson-Weiss syndrome demonstrate that this autosomal dominant form of acrocephalosyndactyly does not map to the ACS III region on 7p or to the acrocephalosyndactyly locus on 5q (Boston type). These findings underline the genetic heterogeneity among the different clinical conditions manifesting with acrocephalosyndactyly.

  11. Genome-wide association analysis in East Asians identifies breast cancer susceptibility loci at 1q32.1, 5q14.3 and 15q26.1

    Cai, Qiuyin; Zhang, Ben; Sung, Hyuna; Low, Siew-Kee; Kweon, Sun-Seog; Lu, Wei; Shi, Jiajun; Long, Jirong; Wen, Wanqing; Choi, Ji-Yeob; Noh, Dong-Young; Shen, Chen-Yang; Matsuo, Keitaro; Teo, Soo-Hwang; Kim, Mi Kyung; Khoo, Ui Soon; Iwasaki, Motoki; Hartman, Mikael; Takahashi, Atsushi; Ashikawa, Kyota; Matsuda, Koichi; Shin, Min-Ho; Park, Min Ho; Zheng, Ying; Xiang, Yong-Bing; Ji, Bu-Tian; Park, Sue K.; Wu, Pei-Ei; Hsiung, Chia-Ni; Ito, Hidemi; Kasuga, Yoshio; Kang, Peter; Mariapun, Shivaani; Ahn, Sei Hyun; Kang, Han Sung; Chan, Kelvin Y. K.; Man, Ellen P. S.; Iwata, Hiroji; Tsugane, Shoichiro; Miao, Hui; Liao, Jiemin; Nakamura, Yusuke; Kubo, Michiaki; Delahanty, Ryan J.; Zhang, Yanfeng; Li, Bingshan; Li, Chun; Gao, Yu-Tang; Shu, Xiao-Ou; Kang, Daehee; Zheng, Wei


    In a three-stage genome-wide association study among East Asian women including 22,780 cases and 24,181 controls, we identified three novel genetic loci associated with breast cancer risk, including rs4951011 at 1q32.1 (in intron 2 of the ZC3H11A gene, P = 8.82 × 10−9), rs10474352 at 5q14.3 (near the ARRDC3 gene, P = 1.67 × 10−9), and rs2290203 at 15q26.1 (in intron 14 of the PRC1 gene, P = 4.25 × 10−8). These associations were replicated in European-ancestry populations including 16,003 cases and 41,335 controls (P = 0.030, 0.004, and 0.010, respectively). Data from the ENCODE project suggest that variants rs4951011 and rs10474352 may be located in an enhancer region and transcription factor binding sites, respectively. This study provides additional insights into the genetics and biology of breast cancer. PMID:25038754

  12. Energetics and Dynamics of the Reactions of O(3P) with Dimethyl Methylphosphonate and Sarin

    Conforti, Patrick F.; Braunstein, Matthew; Dodd, James A.


    Electronic structure and molecular dynamics calculations were performed on the reaction systems O(3P) + sarin and O(3P) + dimethyl methylphosphonate (DMMP), a sarin simulant. Transition state geometries, energies, and heats of reaction for the major reaction pathways were determined at several levels of theory, including AM1, B3LYP/6-311+G(d,p), and CBS-QB3. The major reaction pathways for both systems are similar and include H-atom abstraction, H-atom elimination, and methyl elimination, in rough order from low to high energy. The H-atom abstraction channels have fairly low barriers (˜10 kcal mol-1) and are close to thermoneutral, while the other channels have relatively high energy barriers (>40 kcal mol-1) and a wide range of reaction enthalpies. We have also found a two-step pathway leading to methyl elimination through O-atom attack on the phosphorus atom for DMMP and sarin. For sarin, the two-step methyl elimination pathway is significantly lower in energy than the single-step pathway. We also present results of O(3P) + sarin and O(3P) + DMMP reaction cross sections over a broad range of collision energies (2-10 km s-1 collision velocities) obtained using the direct dynamics method with an AM1 semiempirical potential. These excitation functions are intended as an approximate guide to future hyperthermal measurements, which to our knowledge have not yet examined either of these systems. The reaction barriers, reaction enthalpies, transition state structures, and excitation functions are generally similar for DMMP and sarin, with some moderate differences for methyl elimination energetics, which indicates DMMP will likely be a good substitute for sarin in many O(3P) chemical investigations.

  13. Evolutionary divergence of Ure2pA glutathione transferases in wood degrading fungi.

    Roret, Thomas; Thuillier, Anne; Favier, Frédérique; Gelhaye, Eric; Didierjean, Claude; Morel-Rouhier, Mélanie


    The intracellular systems of detoxification are crucial for the survival of wood degrading fungi. Within these systems, glutathione transferases could play a major role since this family of enzymes is specifically extended in lignolytic fungi. In particular the Ure2p class represents one third of the total GST number in Phanerochaete chrysosporium. These proteins have been phylogenetically split into two subclasses called Ure2pA and Ure2pB. Ure2pB can be classified as Nu GSTs because of shared structural and functional features with previously characterized bacterial isoforms. Ure2pA can rather be qualified as Nu-like GSTs since they exhibit a number of differences. Ure2pA possess a classical transferase activity, a more divergent catalytic site and a higher structural flexibility for some of them, compared to Nu GSTs. The characterization of four members of this Ure2pA subclass (PcUre2pA4, PcUre2pA5, PcUre2pA6 and PcUre2pA8) revealed specific functional and structural features, suggesting that these enzymes have rapidly evolved and differentiated, probably to adapt to the complex chemical environment associated with wood decomposition.

  14. An agent-based incentive mechanism for P2P systems

    ZHANG Jian-feng; WANG Ru-chuan


    With the increasing uses of peer-to-peer (P2P)systems, the problems of fair information and resources sharing become serious. P2P systems are self-organizing,distributed systems with no centralized authority, such as Free-riding and Tragedy of Commons. Because of the voluntary participation and lack of motivation, the information and resources available in P2P networks are extremely variable and unpredictable. This article studied the problems existing in P2P networks and propose a different method to stimulate the peers in P2P networks to share information and resources,using agents so as to improve the performance of P2P networks.And through simulation experiments and analyzing the results,it can be concluded that this mechanism can effectively solve the problems of fair sharing in P2P systems.

  15. Forbidden 2 P- nP and 2 P- nF transitions in the energy spectrum of ultracold Rydberg lithium-7 atoms

    Zelener, B. B.; Saakyan, S. A.; Sautenkov, V. A.; Manykin, E. A.; Zelener, B. V.; Fortov, V. E.


    Forbidden 2 P- nP and 2 P- nF transitions in the ranges of the principal quantum number n = 42-114 and n = 38-48 have been detected in the optical spectra of ultracold highly excited lithium-7 atoms. The presence of forbidden transitions is due to induced external electric fields. The quantum defects and ionization energy obtained in various experiments and predicted theoretically have been discussed.

  16. Effects of the electron correlation and Breit and hyperfine interactions on the lifetime of the 2p$^5$3s states in neutral neon

    Li, Jiguang; Godefroid, Michel; Gaigalas, Gediminas


    In the framework of the multiconfiguration Dirac-Hartree-Fock method, we investigate the transition properties of four excited states in the $2p^53s$ configuration of neutral neon. The electron correlation effects are taken into account systematically by using the active space approach. The effect of higher-order correlation on fine structures is shown. We also study the influence of the Breit interaction and find that it reduces the oscillator strength of the $^3P^o_1 - ^1S_0$ transition by 17%. It turns out that the inclusion of the Breit interaction is essential even for such a light atomic system. Our ab initio calculated line strengths, oscillator strengths and transition rates are compared with other theoretical values and experimental measurements. Good agreement is found except for the $^3P^o_2 - ^1S_0$ M2 transition for which discrepancies of around 15% between theories and experiments remain. In addition, the impact of hyperfine interactions on the lifetimes of the $^3P^o_0$ and $^3P^o_2$ metastable...

  17. Control of sigma virus multiplication by the ref(2)P gene of Drosophila melanogaster: an in vivo study of the PB1 domain of Ref(2)P.

    Carré-Mlouka, A; Gaumer, S; Gay, P; Petitjean, A M; Coulondre, C; Dru, P; Bras, F; Dezélée, S; Contamine, D


    Ref(2)P has been described as one of the Drosophila proteins that interacts with the sigma virus cycle. We generated alleles to identify critical residues involved in the restrictive (inhibiting viral multiplication) or permissive (allowing viral multiplication) character of Ref(2)P. We demonstrate that permissive alleles increase the ability of the sigma virus to infect Drosophila when compared to null alleles and we confirm that restrictive alleles decrease this capacity. Moreover, we have created alleles unfunctional in viral cycling while functional for Ref(2)P fly functions. This type of allele had never been observed before and shows that fly- and virus-related activities of Ref(2)P are separable. The viral status of Ref(2)P variants is determined by the amino-terminal PB1 domain polymorphism. In addition, an isolated PB1 domain mimics virus-related functions even if it is similar to a loss of function toward fly-related activities. The evolutionary tree of the Ref(2)P PB1 domain that we could build on the basis of the natural allele sequences is in agreement with an evolution of PB1 domain due to successive transient selection waves.

  18. 非结构化P2P Overlay拓扑结构分析%Analysis of Topology Structure in Unstructured P2P Overlay

    姜卫; 王观玉; 宋世延


    一个合理的P2P overlay可以为P2P应用提供更好的支持.非结构化P2P overlay的构建比较简单随意,适合于信息发布、即时通讯等节点随时加入退出的情况.构建具有可扩展性和稳健性的网络拓扑,是非结构化P2P overlay的研究热点之一.文章首先介绍了几种典型的非结构化P2P overlay,然后与以往研究不同,通过GT-ITM建模分析了每种P2P overlay拓扑结构的性能,主要分析了不同拓扑结构节点的度、最短路径分布、节点发现概率及结构的健壮性等,该工作对构造合理的P2P overlay拓扑结构和P2P系统的研究具有重要意义.

  19. Photoelectric characteristics of CH3NH3PbI3/p-Si heterojunction

    Yamei, Wu; Ruixia, Yang; Hanmin, Tian; Shuai, Chen


    Organic-inorganic hybrid perovskite CH3NH3PbI3 film is prepared on p-type silicon substrate using the one-step solution method to form a CH3NH3PbI3/p-Si heterojunction. The film morphology and structure are characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The photoelectric properties of the CH3NH3PbI3/p-Si heterojunction are studied by testing the current-voltage (I-V) with and without illumination and capacitance-voltage (C-V) characteristics. It turns out from the I-V curve without illumination that the CH3NH3PbI3/p-Si heterojunction has a rectifier feature with the rectification ratio over 70 at the bias of ±5 V. Also, there appears a photoelectric conversion phenomenon on this heterojunction with a short circuit current (Isc) of 0.16 μA and an open circuit voltage (Voc) of about 10 mV The high frequency C-V characteristic of the Ag/CH3NH3PbI3/p-Si heterojunction turns out to be similar to that of the metal-insulator-semiconductor (MIS) structure, and a parallel translation of the C-V curve along the forward voltage axis is found. This parallel translation means the existence of defects at the CH3NH3PbI3/p-Si interface and positive fixed charges in the CH3NH3PbI3 layer. The defects at the interface of the CH3NH3PbI3/p-Si heterojunction result in the dramatic decline of the Voc. Besides, the C-V test of CH3NH3PbI3 film shows a non-linear dielectric property and the dielectric value is about 4.64 as calculated. Project supported by the Hebei Province Natural Science Foundation of China (No. F2014202184) and the Tianjin Natural Science Foundation of China (No. 15JCZDJC37800).

  20. AFM imaging reveals the assembly of a P2X receptor complex containing P2X2, P2X4 and P2X6 subunits


    Seven P2X purinergic receptor subunits have been identified: P2X1-P2X7. All except P2X6 assemble as homotrimers, and six heteromeric receptors (P2X1/2, P2X1/4, P2X1/5, P2X2/3, P2X2/6 and P2X4/6) have been described. In addition, P2X4 homomers associate with P2X2 or P2X7 homomers as dimers of trimers. The various P2X receptors show individual functional properties, suggesting distinct physiological roles. The overlapping expression of P2X2, P2X4 and P2X6 subunits has been shown in different ce...

  1. Verifier-based Password Authenticated 3P-EKE Protocol using PCLA keys

    Archana Raghuvamshi


    Full Text Available This paper endeavors to present a novel framework for the generic structure of a verifier-based password authenticated Three-Party Encrypted Key Exchange (3P-EKE protocol which yields more efficient protocol than the ones knew before. A previous framework presented by Archana and Premchand is more secured against all types of attacks like password guessing, replay, pre-play, man-in-the-middle attack etc. But unfortunately, this protocol does not solve the problem of a server compromise. These proofs help as inspiration to search for another framework. The framework we offer produces more efficient 3P-EKE protocol, and, in addition, delivers perceptive clarification about the existing attacks that do not solve in the previous framework. Moreover, it allows direct change from a class of verge private-key encryption to a hybrid (symmetric & Asymmetric one without significant overhead.

  2. Mechanism and kinetics for the reaction of O(3P) with DMSO: A theoretical study

    Mandal, Debasish; Bagchi, Sabyasachi; Das, Abhijit K.


    Mechanism and kinetics for the reaction of DMSO with O(3P) have been investigated by M06-2X/MG3S, CBS-QB3 and G4MP2 methods. Four possible reaction pathways are identified. Among them, the O(3P) addition to S-atom followed by CH3 elimination is almost exclusive. Four pre-reactive complexes have been located. AIM theory is used to determine the nature of interactions in these complexes. Considering the formation of pre-reactive complex, the rate constant for major pathway is calculated using transition state theory applied to a two-step mechanism. Enthalpies of formation at 298.15 K (ΔfH°298.15) have been calculated using the composite CBS-QB3, G4MP2 and G3B3 methods.

  3. Multi-reassortant G3P[3] group A rotavirus in a horseshoe bat in Zambia.

    Sasaki, Michihito; Orba, Yasuko; Sasaki, Satoko; Gonzalez, Gabriel; Ishii, Akihiro; Hang'ombe, Bernard M; Mweene, Aaron S; Ito, Kimihito; Sawa, Hirofumi


    Group A rotavirus is a major cause of diarrhoea in humans, especially in young children. Bats also harbour group A rotaviruses, but the genetic backgrounds of bat rotavirus strains are usually distinct from those of human rotavirus strains. We identified a new strain of group A rotavirus in the intestinal contents of a horseshoe bat in Zambia. Whole genome sequencing revealed that the identified virus, named RVA/Bat-wt/ZMB/LUS12-14/2012/G3P[3], possessed the genotype constellation G3-P[3]-I3-R2-C2-M3-A9-N2-T3-E2-H3. Several genome segments of LUS12-14 were highly similar to those of group A rotaviruses identified from humans, cows and antelopes, indicating interspecies transmission of rotaviruses between bats and other mammals with possible multiple genomic reassortment events.

  4. LiNbO3/p+n diode surface acoustic wave memory correlator

    张朝; 水永安; 印建华


    A detailed theoretical analysis of strip-coupled LiNbO3/p+ n diode surface acoustic wave (SAW) memory correlator in the parametric mode is presented. The influence of some important factors on correlation output is analyzed and calculated, including the amplitudes of reference, read and write signal, duration of write signal and doping density of the diode array. The conclusions can be employed for the design of improved strip-coupled SAW memorycorrelators.

  5. Fracture toughness of Al-4%Mg/Al{sub 2}O{sub 3}p composites

    Pestes, R.H. [Hewlett-Packard, Camus, WA 98607 (United States); Kamat, S.V. [Mechanical and Materials Engineering Department, Washington State University, Pullman, WA 99164-2920 (United States); Hirth, J.P. [Mechanical and Materials Engineering Department, Washington State University, Pullman, WA 99164-2920 (United States)


    Fracture toughness tests were carried out on Al-4%Mg/Al{sub 2}O{sub 3}p composites containing different sizes and volume fractions of alumina particulates. The results indicated that the fracture toughness was dependent on the interparticle spacing provided the particulate size was below a critical size. The critical particle size corresponds to the situation wherein the energy release rate on particulate cracking is sufficient to trigger dynamic unstable crack extension. ((orig.))

  6. The Participative Design of an Endoscopy Facility using Lean 3P.

    Smith, Iain


    In the UK, bowel cancer is the second largest cancer killer. Diagnosing people earlier can save lives but demand for endoscopies is increasing and this can put pressure on waiting times. To address this challenge, an endoscopy unit in North East England decided to improve their facilities to increase capacity and create environments that improve the experience of users. This presented a significant opportunity for step change improvement but also a problem in terms of creating designs that meet user requirements whilst addressing structural or space constraints. The Lean design process known as '3P' (standing for the production preparation process) was utilised as a participative design strategy to engage stakeholders in the design of the new department. This involved a time-out workshop (or 3P event) in which Lean and participative design tools were utilised to create an innovative design based on 'point of delivery' (POD) principles. The team created a design that demonstrated an increase in treatment room capacity by 25% and bed capacity by 70% whilst reducing travel distance for patients by 25.8% and staff by 27.1%. This was achieved with an increase in available space of only 13%. The Lean 3P method provided a structured approach for corporate and clinical staff to work together with patient representatives as cross-functional teams. This participative approach facilitated communication and learning between stakeholders about care processes and personal preferences. Lean 3P therefore appears to be a promising approach to improving the healthcare facilities design process to meet user requirements.

  7. miR-142-3p prevents macrophage differentiation during cancer-induced myelopoiesis.

    Sonda, Nada; Simonato, Francesca; Peranzoni, Elisa; Calì, Bianca; Bortoluzzi, Stefania; Bisognin, Andrea; Wang, Ena; Marincola, Francesco M; Naldini, Luigi; Gentner, Bernhard; Trautwein, Christian; Sackett, Sara Dutton; Zanovello, Paola; Molon, Barbara; Bronte, Vincenzo


    Tumor progression is accompanied by an altered myelopoiesis causing the accumulation of immunosuppressive cells. Here, we showed that miR-142-3p downregulation promoted macrophage differentiation and determined the acquisition of their immunosuppressive function in tumor. Tumor-released cytokines signaling through gp130, the common subunit of the interleukin-6 cytokine receptor family, induced the LAP∗ isoform of C/EBPβ transcription factor, promoting macrophage generation. miR-142-3p downregulated gp130 by canonical binding to its messenger RNA (mRNA) 3' UTR and repressed C/EBPβ LAP∗ by noncanonical binding to its 5' mRNA coding sequence. Enforced miR expression impaired macrophage differentiation both in vitro and in vivo. Mice constitutively expressing miR-142-3p in the bone marrow showed a marked increase in survival following immunotherapy with tumor-specific T lymphocytes. By modulating a specific miR in bone marrow precursors, we thus demonstrated the feasibility of altering tumor-induced macrophage differentiation as a potent tool to improve the efficacy of cancer immunotherapy.

  8. $^3P_2$-$^3F_2$ Pairing in Dense Neutron Matter The Spectrum of Solutions

    Zverev, M V; Khodel, V A


    The $^3P_2$-$^3F_2$ pairing model is generally considered to provide an adequate description of the superfluid states of neutron matter at densities some 2-3 times that of saturated symmetrical nuclear matter. The problem of solving the system of BCS gap equations expressing the $^3P_2$-$^3F_2$ model is attacked with the aid of the separation approach. This method, developed originally for quantitative study of S-wave pairing in the presence of strong short-range repulsions, serves effectively to reduce the coupled, singular, nonlinear BCS integral equations to a set of coupled algebraic equations. For the first time, sufficient precision becomes accessible to resolve small energy splittings between the different pairing states. Adopting a perturbative strategy, we are able to identify and characterize the full repertoire of real solutions of the $^3P_2$-$^3F_2$ pairing model, in the limiting regime of small tensor-coupling strength. The P-F channel coupling is seen to lift the striking parametric degeneracie...

  9. Residual Chemosensory Capabilities in Double P2X2/P2X3 Purinergic Receptor Null Mice: Intraoral or Postingestive Detection?

    Hallock, Robert M.; Tatangelo, Marco; Barrows, Jennell


    Mice lacking the purinergic receptors, P2X2 and P2X3 (P2X2/P2X3Dbl−/−), exhibit essentially no tastant-evoked activity in the chorda tympani and glossopharyngeal nerves and substantial loss of tastant-evoked behavior as measured in long-term intake experiments. To assess whether the residual chemically driven behaviors in these P2X2/P2X3Dbl−/− mice were attributable to postingestive detection or oropharyngeal detection of the compounds, we used brief access lickometer tests to assess the behavioral capabilities of the P2X2/P2X3Dbl−/− animals. The P2X2/P2X3Dbl−/− mice showed avoidance to high levels (10 mM quinine and 10–30 mM denatonium benzoate) of classical “bitter”-tasting stimuli in 24-h, 2-bottle preference tests but minimal avoidance of these substances in the lickometer tests, suggesting that the strong avoidance in the intake tests was largely mediated by post-oral chemosensors. Similarly, increases in consumption of 1 M sucrose by P2X2/P2X3Dbl−/− mice in long-term intake tests were not mirrored by increases in consumption of sucrose in lickometer tests, suggesting that sucrose detection in these mice is mediated by postingestive consequences. In contrast, in brief access tests, P2X2/P2X3Dbl−/− mice avoided citric acid and hydrochloric acid at the same concentrations as their wild-type counterparts, indicating that these weak acids activate oropharyngeal chemoreceptors. PMID:19833662

  10. IPTV-RM: A Resources Monitoring Architecture for P2P IPTV Systems


    Resources monitoring is an important problem of the overall efficient usage and control of P2P IPTV systems. The resources of IPTV can include all distributing servers, programs and peers. Several researches have tried to address this issue, but most of them illuminated P2P traffic characterization, identification and user behavior. The main contributions of this paper are twofold. Firstly, a resources monitoring architecture for P2P IPTV systems, IPTV-RM, was presented based on previous work...

  11. In vitro phosphorylation and acetylation of the murine pocket protein Rb2/p130.

    Muhammad Saeed

    Full Text Available The retinoblastoma protein (pRb and the related proteins Rb2/p130 and 107 represent the "pocket protein" family of cell cycle regulators. A key function of these proteins is the cell cycle dependent modulation of E2F-regulated genes. The biological activity of these proteins is controlled by acetylation and phosphorylation in a cell cycle dependent manner. In this study we attempted to investigate the interdependence of acetylation and phosphorylation of Rb2/p130 in vitro. After having identified the acetyltransferase p300 among several acetyltransferases to be associated with Rb2/p130 during S-phase in NIH3T3 cells in vivo, we used this enzyme and the CDK4 protein kinase for in vitro modification of a variety of full length Rb2/p130 and truncated versions with mutations in the acetylatable lysine residues 1079, 128 and 130. Mutation of these residues results in the complete loss of Rb2/p130 acetylation. Replacement of lysines by arginines strongly inhibits phosphorylation of Rb2/p130 by CDK4; the inhibitory effect of replacement by glutamines is less pronounced. Preacetylation of Rb2/p130 strongly enhances CDK4-catalyzed phosphorylation, whereas deacetylation completely abolishes in vitro phosphorylation. In contrast, phosphorylation completely inhibits acetylation of Rb2/p130 by p300. These results suggest a mutual interdependence of modifications in a way that acetylation primes Rb2/p130 for phosphorylation and only dephosphorylated Rb2/p130 can be subject to acetylation. Human papillomavirus 16-E7 protein, which increases acetylation of Rb2/p130 by p300 strongly reduces phosphorylation of this protein by CDK4. This suggests that the balance between phosphorylation and acetylation of Rb2/p130 is essential for its biological function in cell cycle control.

  12. Analysis and Design of Instant Message System Based on P2P

    Shen Zhiwei; Ma Shaowu


    By analyzing current development conditions of mainstream Instant Message technologies and services, some detailed discussion about technical solution and formal operation deployment strategies of P2P Instant Message Services based on Interact is made. Several crucial problems of P2P Instant Message System about services management, interconnection with other instant message, security and profit-making mode are analyzed,, and how to develop P2P IM Services is proposed.

  13. OMAN - A Management Architecture for P2P Service Overlay Networks

    Fiorese, Adriano; Simões, Paulo; Boavida, Fernando


    International audience; In a world where networking services are increasingly being provided by service overlay networks, management of these services at overlay level is becoming crucially important. This paper presents an architecture for services management in P2P Service Overlay Networks (SON). The architecture, named OMAN, takes into account the formation of the P2P SON comprising several different service providers belonging to several different network domains. It uses P2P mechanisms t...

  14. TCLM-P2P: Task Collaboration Logic Model Oriented to P2P Community%TCLM-P2P:面向P2P社区的任务协作逻辑模型

    王杨; 王汝传; 严远亭; 韩志杰; 赵保华


    P2P网络中广泛存在的“free riding”现象使其在任务协作领域的应用受到了极大制约.为了实现P2P网络环境下的有效任务协作,提出了一种具有激励机制的任务协作逻辑模型.基于Agent理论,首先给出了对等体、半对等体、P2P社区等概念;然后在合同网的框架下提出了面向P2P网络社区的任务协作逻辑模型TCLM-P2P(task collaborative logic model oriented to P2P community).相对于传统的任务协作模型,在合理的前提假设条件下,模型给出了模型公理和协作规则.该模型通过基于虚拟积分的协作算法实现了具有激励机制的P2P网络中的任务分配与协作.原型系统的实现及仿真实验结果表明TCLM-P2P模型具有可行性和有效性:不仅能够激励自利节点主动参与到任务分配与协作中;同时也能在一定程度上抑制节点的free riding行为,从而保障了P2P系统的有序工作.%Traditional P2P networks mainly are applied to file sharing and instant message fields. However, how to perform the task collaboration based on P2P community is a challenging job. The former research work indicated that the task collaboration in P2P network had been greatly restricted by free riding behaviors. To realize effective task allocating and task collaborating in P2P network environment, this paper presents a task collaboration logic model oriented to P2P community. Based on agent and multi-agent theory, the paper firstly introduces some concepts including the peer body, half-peer body and P2P community; then the TCLM-P2P is presented including some collaboration axioms and rulers. In order to enhance the incentive mechanism, virtual score becomes the main goal which each peer endeavor pursues. In addition, based on the contract net protocol, a task collaboration algorithm is presented. The proposed algorithm is composed of two phases. One is the task collaboration and the other is the task second bid when some peers fail

  15. An Optimized and Improved Network Trust Model Based on P2P

    Li Wei


    Full Text Available Because of the spring up of the P2P network application, its open, anonymous and self-organized characteristics has offered a path for the spreading of virus and junk data, and the security has aroused people’s common concern. The traditional network trust model dealt with dishonest node has certain limitation, for this, improved P2P network trust model and applied D-S evidence theory to the trusted computing of trust model. Through simulation experiment, the result showed: the improved P2P network trust model efficiently increased the successful trading rate of P2P network and improved the network environment

  16. Survey of Search and Replication Schemes in Unstructured P2P Networks

    Thampi, Sabu M


    P2P computing lifts taxing issues in various areas of computer science. The largely used decentralized unstructured P2P systems are ad hoc in nature and present a number of research challenges. In this paper, we provide a comprehensive theoretical survey of various state-of-the-art search and replication schemes in unstructured P2P networks for file-sharing applications. The classifications of search and replication techniques and their advantages and disadvantages are briefly explained. Finally, the various issues on searching and replication for unstructured P2P networks are discussed.

  17. Phagocytes: A Holistic Defense and Protection Against Active P2P Worms

    Chen, Ruichuan; Lua, Eng Keong; Crowcroft, Jon; Tang, Liyong; Chen, Zhong


    Active Peer-to-Peer (P2P) worms present serious threats to the global Internet by exploiting popular P2P applications to perform rapid topological self-propagation. Active P2P worms pose more deadly threats than normal scanning worms because they do not exhibit easily detectable anomalies, thus many existing defenses are no longer effective. We propose an immunity system with Phagocytes --- a small subset of elected P2P hosts that are immune with high probability and specialized in finding an...

  18. What Happened to P2P Systems? A Special Focus on Content Distribution Systems

    Mohammad Fawzi, Ali


    Peer-to-Peer (P2P) technology has emerged as a new distributed computing paradigm. P2P technology attempts to "harness the powers of the edges of the Internet" by making efficient and effective use of peers (users) at the "edge" of the Internet, by direct interaction between peers of the system. P2P architecture has witnessed lots of interest and research in the latest years because of the popularity of file-sharing applications based on it. Content distribution is an essential P2P applicati...

  19. Research on P2P Overlay Network Model with Small-world Features

    Liu, Hao; Chen, Zhigang


    Topology structure of P2P network decides its system performance. However, the existing P2P network models don’t take clustering and symmetry of nodes into account. Based on the algebra and graph theory method of Cayley graph, this paper proposes a novel P2P overlay network model with small-world features. Its simplicity and symmetry can ensure the self-organization and scalability of P2P network. The results of analysis and experiment shows that this model provides better robustness, h...

  20. miR-342-3p affects hepatocellular carcinoma cell proliferation via regulating NF-κB pathway

    Zhao, Liang; Zhang, Yubao, E-mail:


    Recent research indicates that non-coding microRNAs (miRNAs) help regulate basic cellular processes in many types of cancer cells. We hypothesized that overexpression of miR-342-3p might affect proliferation of hepatocellular carcinoma (HCC) cells. After confirming overexpression of miR-342-3p with qRT-PCR, MTT assay showed that HCC cell proliferation was significantly inhibited by miR-342-3p, and that it significantly decreased BrdU-positive cell proliferation by nearly sixfold. Searching for targets using three algorithms we found that miR-342-3p is related to the NF-κB pathway and luciferase assay found that IKK-γ, TAB2 and TAB3 are miR-342-3p target genes. Results of western blot on extracted nuclear proteins of HepG2 and HCT-116 cells showed that miR-342-3p reduced and miR-342-3p-in increased p65 nuclear levels and qRT-PCR found that NF-κB pathway downstream genes were downregulated by miR-342-3p and upregulated by miR-342-3p-in, confirming that miR-342 targets NF-κB pathway. Overexpression of Ikk-γ, TAB2 and TAB3 partially rescued HCC cells proliferation inhibited by miR-342-3p. Using the GSE54751 database we evaluated expression from 10 HCC samples, which strongly suggested downregulation of miR-342-3p and we also found inverse expression between miR-342-3p and its targets IKK-γ, TAB2 and TAB3 from 71 HCC samples. Our results show that miR-342-3p has a significant role in HCC cell proliferation and is suitable for investigation of therapeutic targets. - Highlights: • MiR-342-3p suppresses hepatocellular carcinoma cell proliferation. • MiR-342-3p targets IKK-γ, TAB2 and TAB3 genes. • MiR-342-3p downregulates NF-kB signaling pathway. • MiR-342-3p is downregulated in clinical hepatocellular carcinoma samples. • The expression of miR-342-3p and its target gene is inversely related.

  1. Measurement of the isotope shift of the 2{{\\rm{p}}}^{4}{}^{3}{{P}}_{2} \\rightarrow 2{{\\rm{p}}}^{3}3{\\rm{p}}{}^{3}{{P}}_{2} two-photon transition of O I and a revision of the triplet energy levels of atomic oxygen

    Marinov, D.; Booth, J.-P.; Drag, C.; Blondel, C.


    Two-photon induced fluorescence of atomic oxygen, which is routinely used for plasma and flame diagnostics, is implemented with two counterpropagating laser beams of sufficient monochromaticity to permit Doppler-free spectroscopy. A single-mode injection-seeded pulsed Ti:sapphire laser has been frequency-doubled twice to produce narrow-band pulsed radiation at wavelengths around 225.6 nm, suitable to excite the ground-state-to-2{{{p}}}33{{p}}{}3{P} two-photon resonance line of oxygen. Accurate measurement of the injection-seeding wavelength provides new data on the excitation energy of the 3{{p}}{}3{P} states. The experiment was done both sequentially and simultaneously on 16O and 18O, which makes it possible to test recent calculations of the isotope shift. Having an absolute measurement of the excitation wave-number from the ground level, which has been the case only twice in previous spectroscopic studies of O I, we can re-examine the energy levels of the subset of triplet states and present an updated set of optimised energy values.

  2. Silane photoabsorption spectra mear the Si 2p thresholds:the geometry of Si 2p excited SiH4

    张卫华; 李家明; 等


    Based on the multiple-scattering self-consistent-field method,we have studied the photoabsorption spectra near the Si 2p thresholds of silane,According to our calculations,the clear assignments of the inner-shell photoabsorption spectra are provided.In comparison with the high-resolution experimental spectra,the geometric structure of the Si 2p-excited SiH4 is recommended to be of a C2v symmetry.More specifically,the Si 2p-excited SiH4** have two bond lengths of 2.50 a.u. and another two bond lengths of 2.77 a.u.,and the corresponding two bond angles are 104.0° and 112.5° respectively.

  3. Institute of Information Network Technology%P2P-SIP融合及P2P-SIP系统设计




  4. MiR-490-3p sensitizes ovarian cancer cells to cisplatin by directly targeting ABCC2

    Tian, Jing; Xu, Yan-Ying; Li, Lian; Hao, Quan


    Cisplatin (CDDP) resistance becomes a large obstacle of the beneficial therapy for patients with ovarian cancer. MicroRNAs (miRNAs) act as post-transcriptional regulators of multiple genes’ expression and have been reported to be involved in multi-drug resistance. The purpose of this study was to determine the roles and molecular mechanism of miR-490-3p in the CDDP resistance in ovarian cancer. We found that miR-490-3p was downregulated in CDDP-resistant OVCAR3/CDDP and SKOV3/CDDP cells, which was due to the hypermethylation of miR-490-3p promoter. Functional studies demonstrated that miR-490-3p increased the cell response to CDDP in OVCAR3, SKOV3 and CDDP-resistant cells, while miR-490-3p inhibition resulted in opposite effects. Luciferase assay, real-time PCR and Western blot as well as immunohistochemistry validated that ABCC2 was a direct target of miR-490-3p and miR-490-3p downregulated ABCC2 expression via binding to its 3’UTR. Importantly, silencing of ABCC2 alleviated CDDP resistance induced by miR-490-3p inhibition, while ABCC2 overexpression restored CDDP resistance inhibited by miR-490-3p. In vivo study showed that miR-490-3p enhanced the cytotoxicity of CDDP. Finally, we found that miR-490-3p was downregulated in CDDP-resistant ovarian cancer tissues, while ABCC2 was upregulated. In conclusion, our data indicate that miR-490-3p enhances CDDP sensitivity of ovarian cancer cells through downregulating ABCC2 expression, and suggest that delivery of miR-490-3p might be a potential therapeutic strategy for patients with CDP-resistant ovarian cancer.

  5. Fabrication of a TiO2-P25/(TiO2-P25+TiO2 nanotubes) junction for dye sensitized solar cells

    Nguyen Huy Hao; Gobinda Gyawali; Tohru Sekino; Soo Wohn Lee


    The dye sensitized solar cell (DSSC), which converts solar light into electric energy, is expected to be a promising renewable energy source for today's world. In this work, dye sensitized solar cells, one containing a single layer and one containing a double layer, were fabricated. In the double layer DSSC structure, the under-layer was TiO2-P25 film, and the top layer consisted of a mixture of TiO2-P25 and TiO2 nanotubes. The results indicated that the efficiency of the DSSC with the double...

  6. Duplicación 5q34q35.3 que involucra el gen NSD1: región delimitada por microarreglos de hibridación genómica comparativa. A propósito de un caso

    AR Vázquez del Campo


    Full Text Available La duplicación de la región 5q34q35.3 se ha asociado a un fenotipo inverso de síndrome de Sotos, por contener el gen NSD1 en doble dosis. Se presenta a una paciente con retraso global del desarrollo, peso y talla bajos y dismorfias. Cuenta con antecedente de corrección quirúrgica por defectos de septación auriculoventricular (CIA y CIV, así como remodelación por antecedente de cráneo en trébol. El cariotipo con bandas G mostró un resultado 46,XX,add(5(q35, y para confirmar el origen del material adicional se utilizó sonda de FISH WCP para el cromosoma 5 [46,XX,add(5(q35.ish dup(5(q35(wcp5+]. Para definir puntos de ruptura y realizar correlación genotipo fenotipo se realizó microarreglo CytoScan HD de Affymetrix, que confirmó la duplicación intersticial de novo de 14Mb 46,XX,add(5(q35. arr[hg19] 5q34q35.3(163,110,984-177,227,216x3,177,259,401- 179,330,764x3,179,346,465 180,719,789x3dn, que contiene 80 genes (USCS genome browser, NCBI36/hg19. La descripción de este caso es importante para complementar la delineación del fenotipo, mediante la correlación con la región de la duplicación a nivel de nucleótidos. Cabe resaltar que la paciente demuestra la importancia y la utilidad de las nuevas técnicas de citogenética molecular, con las cuales es posible el análisis detallado de los genes localizados en la región 5q involucrada, así como su correlación con las manifestaciones clínicas.

  7. HFBP: Identifying P2P Traffic by Host Level and Flow Level Behavior Profiles

    Jinghua Yan


    Full Text Available Recently, Peer-to-peer (P2P networks have been widely applied in streaming media, instant messaging, file sharing and other fields, which have occupied more and more network bandwidth. Accurately identify P2P traffic is very important to management and control P2P traffic. In this paper, we introduce HFBP, a novel P2P identification scheme based on the host level and flow level behavior profiles of P2P traffic. HFBP consists of two stages. In the first stage, we calculate the probability that a host takes part in P2P application by matching its behavior with some host level behavior rules. In the second stage, we compute the probability that a flow belonging to P2P application by comparing the statistical features of each flow in the host with several flow feature profiles. We evaluate HFBP using real traffic traces. The identification accuracy achieves 93.1% and 95.1% in terms of flow and byte respectively. The experimental results prove that HFBP obtains satisfactory performance in identifying P2P traffic. 

  8. Phagocytes: A Holistic Defense and Protection Against Active P2P Worms

    Chen, Ruichuan; Crowcroft, Jon; Tang, Liyong; Chen, Zhong


    Active Peer-to-Peer (P2P) worms present serious threats to the global Internet by exploiting popular P2P applications to perform rapid topological self-propagation. Active P2P worms pose more deadly threats than normal scanning worms because they do not exhibit easily detectable anomalies, thus many existing defenses are no longer effective. We propose an immunity system with Phagocytes --- a small subset of elected P2P hosts that are immune with high probability and specialized in finding and "eating" worms in the P2P overlay. The Phagocytes will monitor their managed P2P hosts' connection patterns and traffic volume in an attempt to detect active P2P worm attacks. Once detected, local isolation, alert propagation and software patching will take place for containment. The Phagocytes further provide the access control and filtering mechanisms for communication establishment between the internal P2P overlay and the external hosts. We design a novel adaptive and interaction-based computational puzzle scheme at ...

  9. Data of evolutionary structure change: 1BFGA-2P23A [Confc[Archive

    Full Text Available 1BFGA-2P23A 1BFG 2P23 A A ---DPKRLYC-KNGG---FFLRIHPDGRVDGVREKSDPH.../confEVID> 0 1BFG A 1BFG...dex> 1BFG A 1BFGA GS...4 HIS CA 324 SER CA 410 LEU CA 389 1BFG... A 1BFGA GVREKSDPHIK

  10. 29 CFR 1917.22 - Hazardous cargo 2 (See § 1917.2(p)).


    ... 29 Labor 7 2010-07-01 2010-07-01 false Hazardous cargo 2 (See § 1917.2(p)). 1917.22 Section 1917.22 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION... § 1917.2(p)). 2 The Department of Transportation and the United States Coast Guard apply...

  11. Data of evolutionary structure change: 1ACMA-2P2GA [Confc[Archive


  12. 2p3d Resonant X-ray emission spectroscopy of cobalt compounds

    van Schooneveld, M.M.


    This manuscript demonstrates that 2p3d resonant X-ray emission spectroscopy (RXES) yields unique information on the chemically relevant valence electrons of transition metal atoms or ions. Experimental data on cobalt compounds and several theories were used hand-in-hand. In chapter 1 2p3d RXES was s

  13. 基于爬行器的大规模P2P IPTV测量%Crawler-Based Measurement of Large Scale P2P IPTV Systems 

    姜志宏; 王晖; 樊鹏翼


    为了解大规模P2P IPTV系统中的用户行为特征和拓扑结构特征等内在信息,开发和部署了一个多协议P2P IPTV爬行器TVCrawer,对3个主流的P2P IPTV系统--PPLive,PPStream和UUSee进行了大量的主动测量,并对P2P IPTV系统中的用户行为和网络拓扑特征进行了分析和比较.主要发现包括:1)P2P IPTV系统的频道在线人数中,有一半以上位于不可达的NAT或者防火墙后面;2)节点动态性的波动范围随频道人数的增加而增加,但是其取值范围具有幂律上限;3)节点会话长度符合广延指数分布;4)PPLive的入度分布属于具有指数截断的幂次分布,PPStream的入度表现为某种分段幂律函数,UUSee的入度接近威布尔分布;5)P2P IPTV系统都是异配网络;6)P2P IPTV系统都表现为小世界网络;7)PPLive网络具有聚类特征,而PPStream和UUSee则不存在明显聚类特征;8)3个系统都表现出类似于无标度网络的鲁棒性特征,而与其他两个系统比较时,PPLive具有更高的故障容错性和更明显的攻击脆弱性.这些测量研究和发现不仅有助于设计出更符合真实网络应用环境的系统或协议,也是实现对P2P IPTV进行监测,引导,控制等方面的重要依据和基础.%In order to gain insight on the topology characteristics of P2P IPTV systems and their online user behavior characteristics, This paper develop and deploys a multiprotocol P2P IPTV network crawler, called TVCrawler, which enables users to launch an in-depth measurement and comparative research for several well-known P2P IPTV systems, including PPLive, PPStream, and UUSee. This paper presents results from experiments and research efforts on these large-scale P2P IPTV overlay graphs. Major findings include 1) more than 50% of online users are unreachable because they lie behind NAT or firewall; 2) fluctuation range of churn increases with thc increase of the population of channel, and there exists a power-law upper bound for

  14. Absolute frequency measurement of the 1S0 - 3P0 transition of 171Yb

    Pizzocaro, Marco; Rauf, Benjamin; Bregolin, Filippo; Milani, Gianmaria; Clivati, Cecilia; Costanzo, Giovanni A; Levi, Filippo; Calonico, Davide


    We report the absolute frequency measurement of the unperturbed transition 1S0 - 3P0 at 578 nm in 171Yb realized in an optical lattice frequency standard. The absolute frequency is measured 518 295 836 590 863.55(28) Hz relative to a cryogenic caesium fountain with a fractional uncertainty of 5.4x10-16 . This value is in agreement with the ytterbium frequency recommended as a secondary representation of the second in the International System of Units.

  15. Pulsar Spin-Down by 3P2 Superfluid Neutron with Field Decay

    LUO Xin-Lian; PENG Qiu-He; CHOU Chih-Kang


    To describe pulsar spin-down, we present a simple combined torque model that takes into account both the standard magnetic dipole radiation and the electromagnetic radiation from the 3P2 superSuid vortex neutrons inside neutron star. Using an ordinary exponential model for the magnetic field decay, we derive an analytical formulae for pulsar evolution tracks. The pulsar evolution on the P-P diagram is quite different from that of the standard magnetic dipole radiation model, especially when the supernuid torque or Geld decay becomes dominant.

  16. Phase transitions in Cd3P2 at high pressures and high temperatures

    Yel'kin, F.S.; Sidorov, V.A.; Waskowska, A.;


    The high-pressure, high-temperature structural behaviour of Cd3P2 has been studied using electrical resistance measurements, differential thermal analysis, thermo baric analysis and X-ray diffraction. At room temperature, a phase transformation is observed at 4.0 GPa in compression....... The experimental zero-pressure bulk modulus of the low-pressure phase is 64.7(7) GPa, which agrees quite well with the calculated value of 66.3 GPa using the tight-binding linear muffin-tin orbital method within the local density approximation. Tentatively, the high-pressure phase has an orthorhombic crystal...

  17. Immunoexpression of the COX-2, p53, and caspase-3 proteins in colorectal adenoma and non-neoplastic mucosa

    Nogueira, Renan Brito; Pires, Andréa Rodrigues Cordovil; Soares, Thélia Maria Santos; Rodrigues, Simone Rabello de Souza; Campos, Mariane Antonieta Menino; Toloi, Giovanna Canato; Waisberg, Jaques


    ABSTRACT Objective: To analyze the immunoexpression of the COX-2, p53, and caspase-3 proteins in colorectal adenomas and non-neoplastic mucosa. Methods: 72 individuals were subjected to colonoscopy, which provided 50 samples of adenomas and 45 samples of non-neoplastic colorectal mucosa. The tissue samples were obtained via the tissue microarray technique and subjected to immunohistochemical analysis using primary anti-p53, anti-COX-2, and anti-caspase-3 antibodies. The positivity and intensity of the immunoreaction were classified. The analyzed variables were as follows: site of the adenomas in the colon, degree of dysplasia, size, and score of positivity and intensity of immunoexpression of the p-53, caspase-3, and COX-2 proteins. Results: The immunoexpression of mutated protein p53 was positive in 30 (60%) adenoma samples and negative in 20 (40%) adenoma samples. The immunoexpression of mutated protein p53 was negative in 39 (86.6%) samples and positive in 6 (13.3%) samples of the non-neoplastic colorectal mucosa (p<0.0001). Significant differences were seen between both the largest size (p=0.006) and the highest degree of dysplasia (p<0.0001) of the adenomas and the intensity of immunoexpression of mutated protein p53. The positivity and intensity of immunoexpression of COX-2 (p=0.14) and caspase-3 (p=0.23) showed no significant differences between the adenomas and the non-neoplastic colorectal mucosa. Conclusion: Mutated protein p53 was hyperexpressed in the adenomas compared with the non-neoplastic mucosa. Greater size and greater degree of dysplasia in the adenomas were associated with higher expression of mutated protein p53. The immunoexpression of COX-2 and caspase-3 in the adenomas did not exhibit a correlation with the anatomical-pathological features of the tumors and did not differ from the corresponding expression levels in the non-neoplastic mucosa. PMID:24488384

  18. SPF-A*: Searching Multimedia Data in Heterogeneous Mobile P2P Network

    Fan Ye


    Full Text Available Mobile P2P (MP2P network has been widely used in everyday life combined with the wireless technology development. However, most previous works concentrate on the assumption that the mobile peers are identical of their inner properties in compositing a MP2P network. However, in current network environment, heterogeneous devices are widely used; they may have different storage sizes, data transmission rate, processing ability and even security levels. In such kind of MP2P Network environment, how to make data transmission effectively is challenge. In this paper, we concentrate on how to effectively obtain a queried multimedia resource and generate a path for such data transmission on the overlay MP2P network, so as to make the resource more effectively transmit among the heterogeneous peers. A new path generating algorithm called SPF-A* (Super Peer First A* is devised, and simulation studies are conducted to validate our proposal.

  19. Solid state synthesis and characterization of iron(Ⅱ)pyrophosphate Fe2P2O7


    Offwhite pure Fe2P2O7 was synthesized through solid phase reaction using Fe2O3 and NH4H2PO4 in argon atmosphere. The reaction products of Fe2O3 and NH4H2PO4 at a series of temperatures from 400 to 900 ℃ were characterized by XRD. Comparison and analysis of XRD patterns of resultant products indicated well-crystallized Fe2P2O7 could be obtained over 630 ℃ and Fe2P2O7 prepared at 700 ℃ was triclinic in cell type. Comparison of the cell parameters proved that the as-prepared Fe2P2O7 belonged to β-Fe2P2O7 in crystal phase and SEM showed its size distribution was 0.5-2 μm.

  20. A Free-Rider Forecasting Model Based on Gray System Theory in P2P Networks

    He Xu


    Full Text Available The aim of this study is to forecast the number of free-riders in P2P networks which can help network managers to know the status of the networks in advance and take appropriate measures to cope with free-riding behavior. Free-riding behavior is common in P2P networks, which has a negative impact on the robustness, availability and stability of the networks. Severe free-riding behavior may lead to the crash of the whole P2P application system. Based on the research of free-riding behavior in P2P networks, this paper constructs a free-rider forecasting model (GST model using Gray System Theory. Simulation experiments show that this model has high feasibility, and can carry out reasonable predictions on the number of free-riders in P2P networks.

  1. Computing and Routing for Trust in Structured P2P Network

    Biao Cai


    Full Text Available Study of trust in P2P network now is focus on how to effectively against various malicious behaviors such as providing fake or misleading feedback about other peers and the management of trust in a P2P environment. But the scotoma of portability that trust peer can join (leave a certain P2P network at anytime and anywhere is seldom discussed. In this paper, a structured topology for trusts management in portable P2P network based on DHT (discrete hash table is proposed first, in which includes trust management strategies and peer operations on certain DHT circle. After that, a novel trust-computing model for the structured P2P network and the main trust decisions in the structured network are introduced too. Effectiveness and practicality of the proposed trust management have been showed in simulation experiments at the end.

  2. A Trust Scheme Based DRM Model for P2P System

    XIAO Shangqin; LU Zhengding; LING Hefei; ZOU Fuhao


    With the maturation of P2P technology, there are more and more challenges to the protection of digital rights.DRM for traditional Client/Server model can not satisfy the requirement of digital right protection of P2P network.With practical application of P2P network technology and new generational DRM technology, the article proposes a DRM model for P2P system based on trust degree.The proposed model shares the secret key to the trusty peers and affords necessary security ensure for digital content delivery based on P2P technology.Mathematic analyses and simulations show that, compared to the current DRM model, the proposed model is more robust toleration of transmit and security.

  3. Research on P2P Overlay Network Model with Small-world Features

    Hao LIU


    Full Text Available Topology structure of P2P network decides its system performance. However, the existing P2P network models don’t take clustering and symmetry of nodes into account. Based on the algebra and graph theory method of Cayley graph, this paper proposes a novel P2P overlay network model with small-world features. Its simplicity and symmetry can ensure the self-organization and scalability of P2P network. The results of analysis and experiment shows that this model provides better robustness, higher enquiry efficiency and better load balance than the existing P2P Overlay Network models such as Chord and CAN. Furthermore, it possesses the property of high clustering.

  4. Convergence of Internet and TV: The Commercial Viability of P2P Content Delivery

    de Boever, Jorn

    The popularity of (illegal) P2P (peer-to-peer) file sharing has a disruptive impact on Internet traffic and business models of content providers. In addition, several studies have found an increasing demand for bandwidth consuming content, such as video, on the Internet. Although P2P systems have been put forward as a scalable and inexpensive model to deliver such content, there has been relatively little economic analysis of the potentials and obstacles of P2P systems as a legal and commercial content distribution model. Many content providers encounter uncertainties regarding the adoption or rejection of P2P networks to spread content over the Internet. The recent launch of several commercial, legal P2P content distribution platforms increases the importance of an integrated analysis of the Strengths, Weaknesses, Opportunities and Threats (SWOT).

  5. Fabrication of a TiO2-P25/(TiO2-P25+TiO2 nanotubes) junction for dye sensitized solar cells

    Nguyen Huy Hao; Gobinda Gyawali; Tohru Sekino; Soo Wohn Lee


    The dye sensitized solar cell (DSSC), which converts solar light into electric energy, is expected to be a promising renewable energy source for today's world. In this work, dye sensitized solar cells, one con-taining a single layer and one containing a double layer, were fabricated. In the double layer DSSC structure, the under-layer was TiO2-P25 film, and the top layer consisted of a mixture of TiO2-P25 and TiO2 nanotubes. The results indicated that the efficiency of the DSSC with the double layer structure was a significant improvement in comparison to the DSSC consisting of only a single film layer. The addition of TiO2-P25 in the top layer caused an improvement in the adsorption of dye molecules on the film rather than on the TiO2 nanotubes only. The presence of the TiO2 nanotubes together with TiO2-P25 in the top layer revealed the enhancement in harvesting the incident light and an improvement of electron transport through the film.

  6. Fabrication of a TiO2-P25/(TiO2-P25+TiO2 nanotubes junction for dye sensitized solar cells

    Nguyen Huy Hao


    Full Text Available The dye sensitized solar cell (DSSC, which converts solar light into electric energy, is expected to be a promising renewable energy source for today's world. In this work, dye sensitized solar cells, one containing a single layer and one containing a double layer, were fabricated. In the double layer DSSC structure, the under-layer was TiO2-P25 film, and the top layer consisted of a mixture of TiO2-P25 and TiO2 nanotubes. The results indicated that the efficiency of the DSSC with the double layer structure was a significant improvement in comparison to the DSSC consisting of only a single film layer. The addition of TiO2-P25 in the top layer caused an improvement in the adsorption of dye molecules on the film rather than on the TiO2 nanotubes only. The presence of the TiO2 nanotubes together with TiO2-P25 in the top layer revealed the enhancement in harvesting the incident light and an improvement of electron transport through the film.

  7. MicroRNA-409-3p inhibits osteosarcoma cell migration and invasion by targeting catenin-δ1.

    Wu, Shifeng; Du, Xinjie; Wu, Manwu; Du, Hechun; Shi, Xiaoliang; Zhang, Tao


    Osteosarcoma is the most common primary bone cancer which is associated with early metastatic potential and poor prognosis. However, the molecular mechanisms underlying osteosarcoma progression are not well characterized. Here, we investigated the role of miR-409-3p in osteosarcoma metastasis. Osteosarcoma tissue showed decreased expression of miR-409-3p compared to adjacent non-tumorous tissue. The expression level of miR-409-3p was negatively correlated with osteosarcoma metastasis. Overexpression of miR-409-3p in osteosarcoma cells (U2OS) inhibited cell migration and invasion. Bioinformatics analysis showed that catenin-δ1 (CTNND1, p120-catenin) is a direct target of miR-409-3p. Overexpression of miR-409-3p repressed the expression of catenin-δ1 in U2OS cells at both mRNA and protein levels. Meanwhile, miR-409-3p repressed the activity of luciferase reporter containing the 3'-untranslated region (3'UTR) of CTNND1 gene. Furthermore, expression of catenin-δ1 rescued the inhibitory effect of miR-409-3p on cell migration and invasion. Altogether, these results indicated that miR-409-3p targets catenin-δ1 to repress osteosarcoma metastasis.

  8. Metalloid tolerance based on phytochelatins is not functionally equivalent to the arsenite transporter Acr3p.

    Wysocki, Robert; Clemens, Stephan; Augustyniak, Daria; Golik, Pawel; Maciaszczyk, Ewa; Tamás, Markus J; Dziadkowiec, Dorota


    Active transport of metalloids by Acr3p and Ycf1p in Saccharomyces cerevisiae and chelation by phytochelatins in Schizosaccharomyces pombe, nematodes, and plants represent distinct strategies of metalloid detoxification. In this report, we present results of functional comparison of both resistance mechanisms. The S. pombe and wheat phytochelatin synthase (PCS) genes, when expressed in S. cerevisiae, mediate only modest resistance to arsenite and thus cannot functionally compensate for Acr3p. On the other hand, we show for the first time that phytochelatins also contribute to antimony tolerance as PCS fully complement antimonite sensitivity of ycf1Delta mutant. Remarkably, heterologous expression of PCS sensitizes S. cerevisiae to arsenate, while ACR3 confers much higher arsenic resistance in pcsDelta than in wild-type S. pombe. The analysis of PCS and ACR3 homologues distribution in various organisms and our experimental data suggest that separation of ACR3 and PCS genes may lead to the optimal tolerance status of the cell.

  9. Overexpression of microRNA‑125a‑3p effectively inhibits the cell growth and invasion of lung cancer cells by regulating the mouse double minute 2 homolog/p53 signaling pathway.

    Li, Shenglei; Li, Xin; Zhao, Huasi; Gao, Ming; Wang, Feng; Li, Wencai


    MicroRNAs (miRs) are a family of small non-coding RNAs that are 21‑24 nucleotides in length. Decreased expression of hsa‑miR‑125a‑3p is observed in a number of patients with non‑small cell lung cancer; however, it is not clear how this miRNA regulates the growth and invasion of lung tumor cells. The aim of the present study was to identify the function of hsa‑miR‑125a‑3p in the growth and invasion of lung cancer cells. The expression of hsa‑miR‑125a‑3p in the A549, NCI‑H460 and SPCA‑1 lung cancer cell lines was analyzed by reverse transcription‑quantitative polymerase chain reaction and the human bronchiolar epithelium cell line (HBE) was used as a control. The results demonstrated that the expression of hsa‑miR‑125a‑3p was significantly lower in NCI‑H460, A549 and SPCA‑1 cells, compared with that in HBE cells. Overexpression of sense miR‑125a‑3p in the A549 lung cancer cell line inhibited cell proliferation for 5‑7 days (Pcancer cells. In addition, overexpression of miR‑125a‑3p in the NCI‑H460 lung cancer cell line markedly induced cell apoptosis, which was detected by fluorescence‑activated cell sorting with annexin V‑fluorescein isothiocyanate/propidium iodide staining. The results of the Transwell migration assay also revealed that transfection of miR‑125a‑3p resulted in decreased migration of lung cancer tumor cells. The pro‑apoptotic gene p53 expression was detected by western blot analysis. The results revealed that the expression of mouse double minute (MDM)‑2 homolog, the principal cellular antagonist of p53, was decreased and p53 expression was upregulated in sense has‑miR‑125a‑3p transfected A549 cells. This was consistent with that observed in NCI‑H460 cells, suggesting that hsa‑miR‑125a‑3p may be involved in the regulation of the MDM2/p53 signaling pathway in lung cancer cells. In conclusion, overexpression of hsa‑miR‑125a‑3p significantly inhibited the proliferation and

  10. Functional Diversification of Fungal Glutathione Transferases from the Ure2p Class

    Anne Thuillier


    Full Text Available The glutathione-S-transferase (GST proteins represent an extended family involved in detoxification processes. They are divided into various classes with high diversity in various organisms. The Ure2p class is especially expanded in saprophytic fungi compared to other fungi. This class is subdivided into two subclasses named Ure2pA and Ure2pB, which have rapidly diversified among fungal phyla. We have focused our analysis on Basidiomycetes and used Phanerochaete chrysosporium as a model to correlate the sequence diversity with the functional diversity of these glutathione transferases. The results show that among the nine isoforms found in P. chrysosporium, two belonging to Ure2pA subclass are exclusively expressed at the transcriptional level in presence of polycyclic aromatic compounds. Moreover, we have highlighted differential catalytic activities and substrate specificities between Ure2pA and Ure2pB isoforms. This diversity of sequence and function suggests that fungal Ure2p sequences have evolved rapidly in response to environmental constraints.

  11. Functional diversification of fungal glutathione transferases from the ure2p class.

    Thuillier, Anne; Ngadin, Andrew A; Thion, Cécile; Billard, Patrick; Jacquot, Jean-Pierre; Gelhaye, Eric; Morel, Mélanie


    The glutathione-S-transferase (GST) proteins represent an extended family involved in detoxification processes. They are divided into various classes with high diversity in various organisms. The Ure2p class is especially expanded in saprophytic fungi compared to other fungi. This class is subdivided into two subclasses named Ure2pA and Ure2pB, which have rapidly diversified among fungal phyla. We have focused our analysis on Basidiomycetes and used Phanerochaete chrysosporium as a model to correlate the sequence diversity with the functional diversity of these glutathione transferases. The results show that among the nine isoforms found in P. chrysosporium, two belonging to Ure2pA subclass are exclusively expressed at the transcriptional level in presence of polycyclic aromatic compounds. Moreover, we have highlighted differential catalytic activities and substrate specificities between Ure2pA and Ure2pB isoforms. This diversity of sequence and function suggests that fungal Ure2p sequences have evolved rapidly in response to environmental constraints.

  12. Automated Simulation P2P Botnets Signature Detection by Rule-based Approach

    Raihana Syahirah Abdullah


    Full Text Available Internet is a most salient services in communication. Thus, companies take this opportunity by putting critical resources online for effective business organization. This has given rise to activities of cyber criminals actuated by botnets. P2P networks had gained popularity through distributed applications such as file-sharing, web caching and network storage whereby it is not easy to guarantee that the file exchanged not the malicious in non-centralized authority of P2P networks. For this reason, these networks become the suitable venue for malicious software to spread. It is straightforward for attackers to target the vulnerable hosts in existing P2P networks as bot candidates and build their zombie army. They can be used to compromise a host and make it become a P2P bot. In order to detect these botnets, a complete flow analysis is necessary. In this paper, we proposed an automated P2P botnets through rule-based detection approach which currently focuses on P2P signature illumination. We consider both of synchronisation within a botnets and the malicious behaviour each bot exhibits at the host or network level to recognize the signature and activities in P2P botnets traffic. The rule-based approach have high detection accuracy and low false positive.

  13. Bandwidth Reduction via Localized Peer-to-Peer (P2P Video

    Ken Kerpez


    Full Text Available This paper presents recent research into P2P distribution of video that can be highly localized, preferably sharing content among users on the same access network and Central Office (CO. Models of video demand and localized P2P serving areas are presented. Detailed simulations of passive optical networks (PON are run, and these generate statistics of P2P video localization. Next-Generation PON (NG-PON is shown to fully enable P2P video localization, but the lower rates of Gigabit-PON (GPON restrict performance. Results here show that nearly all of the traffic volume of unicast video could be delivered via localized P2P. Strong growth in video delivery via localized P2P could lower overall future aggregation and core network bandwidth of IP video traffic by 58.2%, and total consumer Internet traffic by 43.5%. This assumes aggressive adoption of technologies and business practices that enable highly localized P2P video.

  14. MicroRNA-27a-3p Inhibits Melanogenesis in Mouse Skin Melanocytes by Targeting Wnt3a.

    Zhao, Yuanyuan; Wang, Pengchao; Meng, Jinzhu; Ji, Yuankai; Xu, Dongmei; Chen, Tianzhi; Fan, Ruiwen; Yu, Xiuju; Yao, Jianbo; Dong, Changsheng


    MicroRNAs (miRNAs) play an essential role in the regulation of almost all the biological processes, including melanogenesis. MiR-27a-3p is nearly six times higher in white alpaca skin compared to brown skin, which indicates that miR-27a-3p may be a candidate regulator for melanogenesis. Wnt3a plays an important role in promoting melanoblasts to differentiate into melanocytes and melanogenesis. To confirm the function of miR-27a-3p to melanogenesis in mammals, miR-27a-3p mimic, inhibitor and their negative control were transfected into mouse melanocytes. As a result, miR-27a-3p inhibits melanogenesis by repressing Wnt3a at post-transcriptional level. A significant decrease in Wnt3a luciferase activity was observed in 293T cells co-transfected with the matched luciferase reporter vector and pre-miR-27a. Furthermore, the presence of exogenous miR-27a-3p significantly decreased Wnt3a protein expression rather than mRNA and reduced β-catenin mRNA levels in melanocytes. The over-expression of miR-27a-3p significantly increased the melanin content of melanocytes. However, miR-27a-3p inhibitor performs an opposite effect on melanogenesis. Wnt3a is one target of miR-27a-3p. MiR-27a-3p could inhibit Wnt3a protein amount by post-transcriptional regulation and melanogenesis in mouse melanocytes. Previous studies reported that Wnt3a promoted melanogenensis in mouse melanocytes. Thus, miR-27-3p inhibits melanogenesis by repressing Wnt3a protein expression.

  15. MiR-519d-3p suppresses invasion and migration of trophoblast cells via targeting MMP-2.

    Jie Ding

    Full Text Available Our study was approved by the Medical Ethics Committee of Tang Du Hospital, Fourth Military Medical University and complied strictly with national ethical guidelines. Preeclampsia (PE is a specific clinical disorder characterized by gestational hypertension and proteinuria and is a leading cause of maternal and perinatal mortality worldwide. The miR-519d-3p is upregulated in the maternal plasma of patients with PE which indicates a possible association between this microRNA and the pathogenesis of PE. No studies to date have addressed the effect of miR-519d-3p on the invasion and migration of trophoblast cells. In our study, we found that miR-519d-3p expression was elevated in placental samples from patients with PE. In vitro, overexpression of miR-519d-3p significantly inhibited trophoblast cell migration and invasion, whereas transfection of a miR-519d-3p inhibitor enhanced trophoblast cell migration and invasion. Luciferase assays confirmed that matrix metalloproteinase-2 (MMP-2 is a direct target of miR-519d-3p. Quantitative real-time PCR and western blot assays showed that overexpression of miR-519d-3p downregulated MMP-2 mRNA and protein expression. Knockdown of MMP-2 using a siRNA attenuated the increased trophoblast migration and invasion promoted by the miR-519d-3p inhibitor. In placentas from patients with PE or normal pregnancies, a negative correlation between the expression of MMP-2 and miR-519d-3p was observed using the Pearson correlation and linear regression analysis. Our present findings suggest that upregulation of miR-519d-3p may contribute to the development of PE by inhibiting trophoblast cell migration and invasion via targeting MMP-2; miR-519d-3p may represent a potential predictive and therapeutic target for PE.

  16. P2 P 网络借贷的运营与法律监管%Regulation on Online P2P Lending



    The rapid development of online person -to -person lending poses great challenge to its regula-tion.In the United States ,the current regulatory structure for online P 2 P lending involves multiple overseeing agen-cies including the United States Securities and Exchange Commission , various federal bank regulators , the Consum-er Financial Protection Bureau and the state counterparts of all these entities .Under this system , the protection on lenders mainly depends on the securities regulation .From April 2014, P2P lending came under the regulatory man-date of the Financial Conduct Authority.Our country hasn’t started regulation on online P2P lending.Due to the public interests it involves , the necessity for online P2P lending to prevent risk and develop healthily requires its regulation as soon as possible .The sphere of online P2P lending is similar to financial business , so it should be regulated by financial regulatory agency .Under the current financial regulation system in China ,banking regulatory commission should be the authority to regulate on online P 2P lending.%P2P网络借贷的迅猛发展给监管带来了巨大的挑战。在美国,P2P网络借贷实行由证券监管部门、银行监管部门、金融消费者保护局等多个部门共同监管的分散监管体制,对投资者的保护主要是通过证券监管实现的。英国自2014年4月起将P2P网络借贷纳入金融行为局( FCA)的监管范畴。我国尚未对P2P网络借贷予以监管,但P2P行业因涉及公众利益,出于风险防范和其健康发展的需要,监管问题必须尽快予以解决。由于P2 P借贷公司从事的是类金融业务,故应由监管金融业务的机构予以监管。在我国现行的金融监管体制下,对P2 P网络借贷实行统一监管的机构应为银监会。

  17. Functional Diversification of Fungal Glutathione Transferases from the Ure2p Class

    Anne Thuillier; Ngadin, Andrew A.; Cécile Thion; Patrick Billard; Jean-Pierre Jacquot; Eric Gelhaye; Mélanie Morel


    The glutathione-S-transferase (GST) proteins represent an extended family involved in detoxification processes. They are divided into various classes with high diversity in various organisms. The Ure2p class is especially expanded in saprophytic fungi compared to other fungi. This class is subdivided into two subclasses named Ure2pA and Ure2pB, which have rapidly diversified among fungal phyla. We have focused our analysis on Basidiomycetes and used Phanerochaete chrysosporium as a model to c...

  18. A Simple FSPN Model of P2P Live Video Streaming System

    Kotevski, Zoran; Mitrevski, Pece


    Peer to Peer (P2P) live streaming is relatively new paradigm that aims at streaming live video to large number of clients at low cost. Many such applications already exist in the market, but, prior to creating such system it is necessary to analyze its performance via representative model that can provide good insight in the system’s behavior. Modeling and performance analysis of P2P live video streaming systems is challenging task which requires addressing many properties and issues of P2P s...

  19. Mobility Impact in Initializing Ring-Based P2P Systems over MANETs

    Wei Ding


    Full Text Available With the encouragement from success of P2P systems in real world application, recently we have seen active research on synergy of P2P systems and mobile ad hoc networks. The paper proposes a solution for mobility disturbance problem in initialization of ring-based P2P systems over ad hoc networks. It is a decentralized ring construction protocol in presence of mobility. A Mobile Ring Ad-hoc Networks (MRAN protocol is presented. MRAN is an extension of RAN [1] under the mobile condition. Simulation result shows MRAN works well with mobility. Upper bound of maximum speed of moving nodes is investigated in simulation.




    Human chromosomal region 3p12-p23 is proposed to harbor at least three tumor suppressor genes involved in the development of lung cancer, renal cell carcinoma, and other neoplasias. In order to identify one of these genes we defined sequence tagged sites (STSs) specific for 3p13-p24.2 by analyzing a

  1. Product lambda-doublet ratios for the O(3P) + D2 reaction: A mechanistic imprint

    Jambrina, P G; Aldegunde, J; Brouard, M; Aoiz, F J


    In the last decade, the development of theoretical methods have allowed chemists to reproduce and explain almost all of the experimental data associated with elementary atom plus diatom collisions. However, there are still a few examples where theory cannot account yet for experimental results. This is the case for the preferential population of one of the $\\Lambda$-doublet states produced by chemical reactions. In particular, recent measurements of the OD($^2\\Pi$) product of the O($^3$P) + D$_2$ reaction have shown a clear preference for the $\\Pi(A')$ $\\Lambda$-doublet states, in apparent contradiction with {\\em ab initio} calculations, which predict a larger reactivity on the $A"$ potential energy surface. Here we present a method to calculate the $\\Lambda$-doublet ratio when concurrent potential energy surfaces participate in the reaction. It accounts for the experimental $\\Lambda$-doublet populations via explicit consideration of the stereodynamics of the process. Furthermore, our results demonstrate that...

  2. Superplasticity in an Aluminum Alloy 6061/A12O3p Composite


    The superplasticity of an Al2O3p/6061Al composite, fabricated by powder metallurgy techniques,has been investigated. Instead of any special thermomechanical processing or hot rolling, simple hot extrusion has been employed to obtain a fine grained structure before superplastic testing.Superplastic tensile tests were performed at strain rates ranging from 10-2 to 10-4 s-1 and at temperatures from 833 to 893 K. A maximum elongation of 200% was achieved at a temperature of 853 K and an initial strain rate of 1.67×10-3 s-1. The highest value obtained for the strain rate sensitivity index (m) was 0.32. Differential scanning calorimeter was used to ascertain the possibility of any partial melting in the vicinity of optimum superplastic temperature. These results suggested that no liquid phase existed where maximum elongation was achieved and deformation took place entirely in the solid state.

  3. Mechanisms and Kinetics of Radical Reaction of O(1D,3P) + HCN System

    HUANG Yu-Cheng; DU Jin-Yan; JU Xue-Hai; YE Shi-Yong; ZHOU Tao


    The reaction of HCN with O(1D, 3P) radical has been investigated by density functional theory (DFT) and ab initio methods. The stationary points on the reaction paths(reactants, intermediates and products) were optimized at the (U)B3LYP/aug-cc-pVTZ level.Single-point calculations were performed at the (U)QCISD(T)/aug-cc-pVTZ level for the optimized structures and all the total energies were corrected by zero-point energy. It is shown that there exist three competing mechanisms of oxygen attacking nitrogen O→N, oxygen attacking carbon O→C and oxygen attacking hydrogen O→H. The rate constants were obtained via Eyring transition-state theory in the temperature range of 600~2000 K. The linear relationship between lnk and 1/T was presented. The results show that path 1 is the main reaction channel and the product of NCO + H is predominant.

  4. Narrow-linewidth cooling of $^{6}$Li atoms using the 2S-3P transition

    Chen, Hao-Ze; Wu, Yu-Ping; Liu, Xiang-Pei; Wang, Xiao-Qiong; Chen, Yu-Ao; Pan, Jian-Wei


    We report on a narrow-linewidth cooling of $^{6}$Li atoms using the $2S_{1/2}\\to 3P_{3/2}$ transition in the ultraviolet (UV) wavelength regime. By combining the traditional red magneto-optical trap (MOT) at 671 nm and the UV MOT at 323 nm, we obtain a cold sample of $1.3\\times10^9$ atoms with a temperature of 58 $\\mu$K. Furthermore, we demonstrate a high efficiency magnetic transport for $^{6}$Li atoms with the help of the UV MOT. Finally, we obtain $8.1\\times10^8$ atoms with a temperature of 296 $\\mu$K at a magnetic gradient of 198 G/cm in the science chamber with a good vacuum environment and large optical access.

  5. The Number of Solutions of the Diophantine Equation x2 + p2 =yn%Diophantine方程x2+p2=yn的解数



    Let p be a fixed odd prime,and let N(p) denote the number of positive integer solutions (x,y,n) of the equation x2 +p2 =yn satisfying gcd(x,y)=1 and n > 2.In this paper we prove that if p ≠ 7,then N(p) < log p.%对于给定的奇素数p,设N(p)为方程x2+ p2=yn满足gcd(x,y)=1和n>2的正整数解(x,y,n)的个数,本文证明了:当p≠7时,N(p) <log p.

  6. Locating Highly Connected Nodes in P2P Networks with Heterogeneous Structures

    ZHANG Haoxiang; ZHANG Lin; SHAN Xiuming; Victor O. K. LI


    Peer-to-peer (P2P) networks aggregate enormous storage and processing resources while mini-mizing entry and scaling costs. Gnutella-like P2P networks are complex heterogeneous networks, in which the underlying overlay topology has a power-law node degree distribution. While scale-free networks have great robustness against random failures, they are vulnerable to deliberate attacks where highly connected nodes are eliminated. Since high degree nodes play an important role in maintaining the connectivity, this paper presents an algorithm based on random walks to locate high degree nodes in P2P networks. Simula-tions demonstrate that the algorithm performs well in various scenarios and that heterogeneous P2P net-works are very sensitive to deliberate attacks.

  7. Pre-Paid Charging System for Sip-P2P Commercial Applications

    Damian Nowak


    Full Text Available The new SIP-P2P approach brings many advantages like the improved reliability and the ease of setup.However, Communications Service Providers (CSPs offering VoIP services seem to be afraid to losetheir profits due to the introduction of SIP-P2P. Claimed is the lack of session establishment controlleading to revenue leakages. In this work we introduce a charging system for SIP-P2P that assures CSPs’revenue from using SIP-based peer-to-peer communications commercially. The proposed solutionconsists of a new “carrier-grade P2P network” concept, software modification of existing equipment andgives some charging application design guidelines. An application prototype was implemented toexamine the concept. Additionally, experiments were done to provide the methodology for systemdimensioning.

  8. Experimental Determination of the Lifetime of the Helium 2P13 1S01 Transition

    Dall, R. G.; Baldwin, K. G. H.; Byron, L. J.; Truscott, A. G.


    We present the first experimental determination of the 2P13 1S01 transition rate in helium and compare this measurement with theoretical quantum-electrodynamic predictions. The experiment exploits the very long (˜1minute) confinement times obtained for atoms magneto-optically trapped in an apparatus used to create a Bose-Einstein condensate of metastable (2S13) helium. The 2P13 1S01 transition rate is measured directly from the decay rate of the cold atomic cloud following 1083 nm laser excitation from the 2S13 to the 2P13 state, and from accurate knowledge of the 2P13 population. The value obtained is 177±8s-1, which agrees very well with theoretical predictions, and has an accuracy that compares favorably with measurements for the same transition in heliumlike ions higher in the isoelectronic sequence.

  9. A Local Scalable Distributed EM Algorithm for Large P2P Networks

    National Aeronautics and Space Administration — his paper describes a local and distributed expectation maximization algorithm for learning parameters of Gaussian mixture models (GMM) in large peer-to-peer (P2P)...

  10. CHC: A Robust P2P Overlay Network with Simple Routing and Small-World Features

    Wenjun Xiao


    Full Text Available Almost all recent researches on P2P systems focus on howto build a highly usable P2P overlay network. Researchersinclude small routing table, short query path and goodrobustness into their design objectives of overlay topology. Inthis paper, we present a general group theory method anddefine a new Cayley graph. Based on this Cayley graph, wepropose a novel P2P overlay network called CHC, which hassimple routing (searching scheme and many other excellentproperties such as short query path, high clustering and goodrobustness because of its symmetry. The performance isevaluated by simulation to show that CHC possesses shorterquery path length and higher clustering and better robustnessthan several popular P2P overlay networks such as Chord andUlysses.

  11. MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1

    Gu, Haihua; Yang, Tao; Fu, Shaozi; Chen, Xiaofan; Guo, Lei; Ni, Yiming, E-mail:


    Highlights: • We examined the level of miR-490-3p in A549 lung cancer cells compared with normal bronchial epithelial cell line. • We are the first to show the function of miR-490-3p in A549 lung cancer cells. • We demonstrate CCND1 may be one of the targets of miR-490-3p. - Abstract: MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate the translation of messenger RNAs by binding their 3′-untranslated region (3′UTR). In this study, we found that miR-490-3p is significantly down-regulated in A549 lung cancer cells compared with the normal bronchial epithelial cell line. To better characterize the role of miR-490-3p in A549 cells, we performed a gain-of-function analysis by transfecting the A549 cells with chemically synthesized miR-490-3P mimics. Overexpression of miR-490-3P evidently inhibits cell proliferation via G1-phase arrest. We also found that forced expression of miR-490-3P decreased both mRNA and protein levels of CCND1, which plays a key role in G1/S phase transition. In addition, the dual-luciferase reporter assays indicated that miR-490-3P directly targets CCND1 through binding its 3′UTR. These findings indicated miR-490-3P could be a potential suppressor of cellular proliferation.

  12. H2S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expression

    Li, Xing-Hui; Xue, Wen-Long; Wang, Ming-Jie; Zhou, Yu; Zhang, Cai-Cai; Sun, Chen; Zhu, Lei; Liang, Kun; Chen, Ying; Tao, Bei-Bei; Tan, Bo; Yu, Bo; Zhu, Yi-Chun


    The aims of the present study are to determine whether hydrogen sulfide (H2S) is involved in the expression of endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) production, and to identify the role of microRNA-455-3p (miR-455-3p) during those processes. In cultured human umbilical vein endothelial cells (HUVECs), the expression of miR-455-3p, eNOS protein and the NO production was detected after administration with 50 μM NaHS. The results indicated that H2S could augment the expression of miR-455-3p and eNOS protein, leading to the increase of NO level. We also found that overexpression of miR-455-3p in HUVECs increased the protein levels of eNOS whereas inhibition of miR-455-3p decreased it. Moreover, H2S and miR-455-3p could no longer increase the protein level of eNOS in the presence of proteasome inhibitor, MG-132. In vivo, miR-455-3p and eNOS expression were considerably increased in C57BL/6 mouse aorta, muscle and heart after administration with 50 μmol/kg/day NaHS for 7 days. We also identified that H2S levels and miR-455-3p expression increased in human atherosclerosis plaque while H2S levels decreased in plasma of atherosclerosis patients. Our data suggest that the stability of eNOS protein and the NO production could be regulated by H2S through miR-455-3p. PMID:28322298

  13. Adaptive Routing Algorithms in Unstructured Peer-to-Peer (P2P Systems

    Achmad Nizar Hidayanto,


    Full Text Available There are a variety of peer-to-peer (P2P systems for sharing documents currently available. According to their data organization, P2P systems are classified into two categories: structured andunstructured P2P systems. In structured P2P systems, peers are organized according to some mapping techniques, e.g. hashing function. Whereas in unstructured P2P system, peers are connected to each others randomly; resources are not moved to other peers but hosted on site. Unstructured P2P systems offer a more flexible and autonomous environment, as they require less control for the placement of resources and peers. This work focuses on experimenting on unstructured P2P systems. The challenge in unstructured P2P system is designing routing strategies that lead the user in finding the documents needed. Routing strategies in unstructured P2P system need to consider the dynamic aspects of P2P systems; peers are dynamic and constantly joining and leaving the system, network load changescontinuously and resources are added and removed over the time. Therefore, the routing strategy must adapt to such changes to maintain its performance. We propose routing strategies that adapt to these changes through learning mechanisms. The learning mechanisms are conducted by observing the internal and external behaviors of the system. Internal behaviors reflect the internal state of peers such as peers’ interest and collection. External behaviors reflect the external state of the system such as network load. In order to measure the performance of the proposed routing algorithms, some common performance measurements are used. These are “response time” and “number of messages generated” or what is commonly referred to as efficiency, “number of answered and satisfied queries” and the “similarity of documents” or what is commonly referred to as effectiveness of retrieval system. The experiment results show that the proposed algorithms are capable of adapting to

  14. Quasi-elastic scattering, RPA, 2p2h and neutrino--energy reconstruction

    Nieves, J; Sanchez, F; Vacas, M J Vicente


    We discuss some nuclear effects, RPA correlations and 2p2h (multinucleon) mechanisms, on charged-current neutrino-nucleus reactions that do not produce a pion in the final state. We study a wide range of neutrino energies, from few hundreds of MeV up to 10 GeV. We also examine the influence of 2p2h mechanisms on the neutrino energy reconstruction.

  15. Structure And Function of the Yeast U-Box-Containing Ubiquitin Ligase Ufd2p

    Tu, D.; Li, W.; Ye, Y.; Brunger, A.T.


    Proteins conjugated by Lys-48-linked polyubiquitin chains are preferred substrates of the eukaryotic proteasome. Polyubiquitination requires an activating enzyme (E1), a conjugating enzyme (E2), and a ligase (E3). Occasionally, these enzymes only assemble short ubiquitin oligomers, and their extension to full length involves a ubiquitin elongating factor termed E4. Ufd2p, as the first E4 identified to date, is involved in the degradation of misfolded proteins of the endoplasmic reticulum and of a ubiquitin-{beta}-GAL fusion substrate in Saccharomyces cerevisiae. The mechanism of action of Ufd2p is unknown. Here we describe the crystal structure of the full-length yeast Ufd2p protein. Ufd2p has an elongated shape consisting of several irregular Armadillo-like repeats with two helical hairpins protruding from it and a U-box domain flexibly attached to its C terminus. The U-box of Ufd2p has a fold similar to that of the RING (Really Interesting New Gene) domain that is present in certain ubiquitin ligases. Accordingly, Ufd2p has all of the hallmarks of a RING finger-containing ubiquitin ligase: it associates with its cognate E2 Ubc4p via its U-box domain and catalyzes the transfer of ubiquitin from the E2 active site to Ufd2p itself or to an acceptor ubiquitin molecule to form unanchored diubiquitin oligomers. Thus, Ufd2p can function as a bona fide E3 ubiquitin ligase to promote ubiquitin chain elongation on a substrate.

  16. Secure Mobile Agent for Telemedicine Based on P2P Networks

    Hsu, Wen-Shin; Pan, Jiann-I


    Exploring intelligent mobile agent (MA) technology for assisting medical services or transmitting personal patient-health information in telemedicine applications has been widely investigated. Conversely, peer-to-peer (P2P) networking has become one of the most popular applications used in the Internet because of its benefits for easy-to-manage resources and because it balances workloads. Therefore, constructing an agent-based telemedicine platform based on P2P networking architecture is nece...

  17. An Efficient, Scalable and Robust P2P Overlay for Autonomic Communication

    Li, Deng; Liu, Hui; Vasilakos, Athanasios

    The term Autonomic Communication (AC) refers to self-managing systems which are capable of supporting self-configuration, self-healing and self-optimization. However, information reflection and collection, lack of centralized control, non-cooperation and so on are just some of the challenges within AC systems. Since many self-* properties (e.g. selfconfiguration, self-optimization, self-healing, and self-protecting) are achieved by a group of autonomous entities that coordinate in a peer-to-peer (P2P) fashion, it has opened the door to migrating research techniques from P2P systems. P2P's meaning can be better understood with a set of key characteristics similar to AC: Decentralized organization, Self-organizing nature (i.e. adaptability), Resource sharing and aggregation, and Fault-tolerance. However, not all P2P systems are compatible with AC. Unstructured systems are designed more specifically than structured systems for the heterogeneous Internet environment, where the nodes' persistence and availability are not guaranteed. Motivated by the challenges in AC and based on comprehensive analysis of popular P2P applications, three correlative standards for evaluating the compatibility of a P2P system with AC are presented in this chapter. According to these standards, a novel Efficient, Scalable and Robust (ESR) P2P overlay is proposed. Differing from current structured and unstructured, or meshed and tree-like P2P overlay, the ESR is a whole new three dimensional structure to improve the efficiency of routing, while information exchanges take in immediate neighbors with local information to make the system scalable and fault-tolerant. Furthermore, rather than a complex game theory or incentive mechanism, asimple but effective punish mechanism has been presented based on a new ID structure which can guarantee the continuity of each node's record in order to discourage negative behavior on an autonomous environment as AC.

  18. P2P network traffic control mechanism based on global evaluation values

    XU Xiao-long; WANG Ru-chuan


    Peer-to-peer (P2P) computing technology has been widely used on the Internet to exchange data. However, it occupies much network bandwidth, and thus greatly influences traditional business on the Interact. Besides, problems about free-riders and 'tragedy of the commons' in the P2P environment estrange from it P2P users who constantly contribute to the network with quality resources. This article proposes a new P2P network traffic control mechanism based on global evaluation values. It aims to help individual users to avoid peak traffic time as much as possible, ease network congestion and protect traditional business on the Interact, as well as differentiating priority grades of peers according to their contributions and stimulating them to share their valuable resources actively. This article first analyzes the current state of network traffic, and then elaborates on P2P network traffic control policies and proposes the peer's priority level differentiation mechanism based on global evaluation values. Finally,after the testing results and analysis of the proposed P2P network traffic control mechanism are discussed, conclusions are drawn.

  19. Fine-grained P2P traffc classifi cation by simply counting flows

    Jie HE; Yue-xiang YANG; Yong QIAO; Wen-ping DENG


    The continuous emerging of peer-to-peer (P2P) applications enriches resource sharing by networks, but it also brings about many challenges to network management. Therefore, P2P applications monitoring, in particular, P2P traffic classifi cation, is becoming increasingly important. In this paper, we propose a novel approach for accurate P2P traffic classifi cation at a fi ne-grained level. Our approach relies only on counting some special fl ows that are appearing frequently and steadily in the traffic generated by specifi c P2P applications. In contrast to existing methods, the main contribution of our approach can be summarized as the following two aspects. Firstly, it can achieve a high classifi cation accuracy by exploiting only several generic properties of fl ows rather than complicated features and sophisticated techniques. Secondly, it can work well even if the classifi cation target is running with other high bandwidth-consuming applications, outperforming most existing host-based approaches, which are incapable of dealing with this situation. We evaluated the performance of our approach on a real-world trace. Experimental results show that P2P applications can be classifi ed with a true positive rate higher than 97.22%and a false positive rate lower than 2.78%.

  20. Strategies for P2P connectivity in reconfigurable converged wired/wireless access networks.

    Puerto, Gustavo; Mora, José; Ortega, Beatriz; Capmany, José


    This paper presents different strategies to define the architecture of a Radio-Over-Fiber (RoF) Access networks enabling Peer-to-Peer (P2P) functionalities. The architectures fully exploit the flexibility of a wavelength router based on the feedback configuration of an Arrayed Waveguide Grating (AWG) and an optical switch to broadcast P2P services among diverse infrastructures featuring dynamic channel allocation and enabling an optical platform for 3G and beyond wireless backhaul requirements. The first architecture incorporates a tunable laser to generate a dedicated wavelength for P2P purposes and the second architecture takes advantage of reused wavelengths to enable the P2P connectivity among Optical Network Units (ONUs) or Base Stations (BS). While these two approaches allow the P2P connectivity in a one at a time basis (1:1), the third architecture enables the broadcasting of P2P sessions among different ONUs or BSs at the same time (1:M). Experimental assessment of the proposed architecture shows approximately 0.6% Error Vector Magnitude (EVM) degradation for wireless services and 1 dB penalty in average for 1 x 10(-12) Bit Error Rate (BER) for wired baseband services.

  1. miR-409-3p sensitizes colon cancer cells to oxaliplatin by inhibiting Beclin-1-mediated autophagy.

    Tan, Shifan; Shi, Huijuan; Ba, Mingchen; Lin, Shengqv; Tang, Hongsheng; Zeng, Xiaoqi; Zhang, Xiangliang


    The chemoresistance of colon cancer cells limits the efficacy of chemotherapy. miR-409-3p has been shown to be downregulated in various types of cancer. In the present study, we examined the role of miR-409-3p in colon cancer as well as the effects of miR‑409-3p on the sensitivity of colon cancer cells to oxaliplatin. The expression of miR-409 was significantly downregulated in the human colon cancer cell lines compared with the normal colon epithelial cells. Importantly, the miR-409-3p expression levels were lower in human colon cancer patient samples than in normal colon tissues. Moreover, we observed a negative correlation between the miR‑409-3p levels and resistance to oxaliplatin: the oxaliplatin-resistant colon cancer cells exhibited significantly downregulated miR‑409-3p levels, but higher autophagic activity than the oxaliplatin-sensitive cells. Using bioinformatics analysis, we predicted that miR‑409-3p miRNA binds to the key autophagy gene encoding Beclin-1. Our findings indicated that the overexpression of miR‑409-3p inhibited Beclin-1 expression and autophagic activity by binding to the 3'-untranslated region of Beclin-1 mRNA. In addition, the overexpression of miR‑409-3p enhanced the chemosensitivity of the oxaliplatin-sensitive and oxaliplatin-resistant colon cancer cells. The restoration of Beclin-1 abrogated these effects of miR‑409-3p. In a xenograft model using nude mice, we examined the effects of miR‑409-3p on tumor growth during chemotherapy. miR‑409-3p overexpression sensitized the tumor to chemotherapy, while inhibiting chemotherapy-induced autophagy in a manner dependent on Beclin-1. The findings of our study suggest that miR-409-3p is capable of enhancing the chemosensitivity of colon cancer cells by inhibiting Beclin-1-mediated autophagy.

  2. P2P-based botnets: structural analysis, monitoring, and mitigation

    Yan, Guanhua [Los Alamos National Laboratory; Eidenbenz, Stephan [Los Alamos National Laboratory; Ha, Duc T [UNIV AT BUFFALO; Ngo, Hung Q [UNIV AT BUFFALO


    Botnets, which are networks of compromised machines that are controlled by one or a group of attackers, have emerged as one of the most serious security threats on the Internet. With an army of bots at the scale of tens of thousands of hosts or even as large as 1.5 million PCs, the computational power of botnets can be leveraged to launch large-scale DDoS (Distributed Denial of Service) attacks, sending spamming emails, stealing identities and financial information, etc. As detection and mitigation techniques against botnets have been stepped up in recent years, attackers are also constantly improving their strategies to operate these botnets. The first generation of botnets typically employ IRC (Internet Relay Chat) channels as their command and control (C&C) centers. Though simple and easy to deploy, the centralized C&C mechanism of such botnets has made them prone to being detected and disabled. Against this backdrop, peer-to-peer (P2P) based botnets have emerged as a new generation of botnets which can conceal their C&C communication. Recently, P2P networks have emerged as a covert communication platform for malicious programs known as bots. As popular distributed systems, they allow bots to communicate easily while protecting the botmaster from being discovered. Existing work on P2P-based hotnets mainly focuses on measurement of botnet sizes. In this work, through simulation, we study extensively the structure of P2P networks running Kademlia, one of a few widely used P2P protocols in practice. Our simulation testbed incorporates the actual code of a real Kademlia client software to achieve great realism, and distributed event-driven simulation techniques to achieve high scalability. Using this testbed, we analyze the scaling, reachability, clustering, and centrality properties of P2P-based botnets from a graph-theoretical perspective. We further demonstrate experimentally and theoretically that monitoring bot activities in a P2P network is difficult

  3. Unexpected Reactivity of [(η(5) -1,2,4-tBu3 C5 H2 )Ni(η(3) -P3 )] towards Main Group Nucleophiles and by Reduction.

    Mädl, Eric; Balázs, Gábor; Peresypkina, Eugenia V; Scheer, Manfred


    The reduction of [Cp'''Ni(η(3) -P3 )] (1; Cp'''=η(5) -1,2,4-tBu3 C5 H2 ) with potassium produces the complex anion [(Cp'''Ni)2 (μ,η(2:2) -P8 )](2-) (2), which contains a realgar-like P8 unit. The anionic triple-decker sandwich complex [(Cp'''Ni)2 (μ,η(3:3) -P3 )](-) (3) with a cyclo-P3 middle deck is obtained when 1 is treated with NaNH2 as a nucleophile. Na[3] can subsequently be oxidized with AgOTf to the neutral triple-decker complex [(Cp'''Ni)2 (μ,η(3:3) -P3 )] (4). In contrast, 1 reacts with LiPPh2 to give the anionic compound [(Cp'''Ni)2 (μ,η(2:2) -P6 PPh2 )](-) (5), a complex containing a bicyclic P7 fragment capped by two Cp'''Ni units. Protonation of Li[5] with HBF4 leads to the neutral complex [(Cp'''Ni)2 (μ,η(2:2) -(HP6 PPh2 )] (6). Adding LiNMe2 to 1 results in [Cp'''Ni(η(2) -P3 NMe2 )](-) (7) becoming accessible, a complex which forms as a result of nucleophilic attack at the cyclo-P3 ring of 1. The complexes K2 [2], Na[3], 4, 6, and Li[7] were fully characterized and their structures determined by single-crystal X-ray diffraction.

  4. Multifunctional g3p-peptide tag for current phage display systems.

    Beckmann, C; Haase, B; Timmis, K N; Tesar, M


    We have previously described a monoclonal antibody (mAb), 10C3, directed against the gene-3 protein (g3p) of filamentous phage M13, which was produced to study g3p fusion protein expression in Escherichia coli and its incorporation in the phage capsid [Tesar, M., Beckmann, C., Röttgen, P., Haase, B., Faude, U., Timmis, K., 1995. Monoclonal antibody against pIII of filamentous phage: an immunological tool to study pIII fusion protein expression in phage display systems. Immunology 1, 53-54]. In this study we report mapping of the antigenic epitope of the mAb 10C3, by means of short overlapping peptide-sequences [Frank, R., Overwin, H., 1996. Spot synthesis. In: Morris, G.E. (Ed.), Methods in Molecular Biology, Vol. 66: Epitope Mapping Protocols. Humana Press, Totowa, NJ, pp. 149-169.] comprising the C-terminal half of the g3-protein. A minimal recognizable peptide was found which is represented in the 11 amino acid sequence from positions 292 to 302 of g3p [Wezenbeek van, P.M.G.P., Hulsebos, T.J.M., Schoenmakers, J.G.G., 1980. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. Gene 11, 129-148]. In order to use the antibody also for detection and purification of recombinant proteins, such as single chain antibodies, the epitope was introduced as a tag sequence into the phagemid pHEN1 [Hoogenboom, H.R., Griffith, A.D., Johnson, K., Chiswell, D.J., Hudson, P., Winter, G., 1991. Multi-subunit proteins on the surface of the filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acid Res. 19, 4133-4137; Nissim, A., Hoogenboom, H.R., Tomlinson, I.M., Flynn, G., Midgley, C., Lane, D., Winter, G., 1994. Antibody fragments from a single pot phage display library as immunochemical reagents. EMBO J. 13 (3) 692-698]. Purified single chain antibodies containing this tag were detectable down to a concentration of 2 ng ml(-1) under non-denaturing conditions (ELISA) or 4 ng per lane on immunoblots

  5. miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200

    Fang, Wang; Shu, Shan; Yongmei, Li; Endong, Zhu; Lirong, Yin; Bei, Sun


    Cervical cancer (CC) is a malignant solid tumor, which is one of the main causes of morbidity and mortality in women. Persistent High-risk human papillomavirus (hrHPV) infection is closely related to cervical cancer and autophagy has been suggested to inhibit viral infections. miRNAs have been reported to regulate autophagy in many solid tumors with many studies implicating miR-224-3p in the regulation of autophagy. In this study, we performed a miRNA microarray analysis on CC tissues and found that a large number of miRNAs with differential expressions in hrHPV-infected tissues. We identified miR-224-3p as a candidate miRNA selectively up regulated in HPV-infected tissues and cell lines. Further analysis revealed that miR-224-3p regulates autophagy in cervical cancer tissues and cell lines. While the overexpression of miR-224-3p inhibits autophagy in HPV-infected cells, knocking down endogenous miR-224-3p increases autophagy activity in the same cells. In addition, we found that miR-224-3p directly inhibits the expression of autophagy related gene, FAK family-interacting protein of 200 kDa (FIP200). In summary, we found that miR-224-3p regulates autophagy in hrHPV-induced cervical cancer cells through targeting FIP200 expression. PMID:27615604

  6. MicroRNA-338-3p suppresses tumor growth of esophageal squamous cell carcinoma in vitro and in vivo.

    Li, Xinyu; Li, Zhihong; Yang, Guiyun; Pan, Zhenxiang


    Accumulating evidence has shown that microRNAs (miRNAs) are aberrantly expressed in human esophageal squamous cell carcinoma (ESCC) and are crucial in tumorigenesis, among which miR‑338‑3p has been examined to be downregulated in patients with ESCC. However, the role of miR‑338‑3p in ESCC remains to be elucidated. In the present study, the role of miR‑338‑3p on the growth and survival of an ESCC cell line was determined with several in vitro approaches and in nude mouse models. It was determined that miR‑338‑3p expression was frequently downregulated in ESCC tissue compared with corresponding adjacent non‑tumor tissue, and that its expression was significantly correlated with tumor stage and metastasis. Overexpression of miR‑338‑3p in ESCC cells suppressed cell proliferation, colony formation, migration and invasion, and induced cell arrest at the G0/G1 stage and cell apoptosis in vitro. In addition, it was demonstrated that overexpression of miR‑338‑3p significantly suppresses tumor growth of xenograft tumors in mice (PESCC, and its dysregulation may be involved in the initiation and development of human ESCC. In addition, it was suggested that miR‑338‑3p may be a potential therapeutic agent for treatment of ESCC.

  7. miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression

    Yu, Peng; Wu, Dingguo; You, Yu; Sun, Jing; Lu, Lele; Tan, Jiaxing; Bie, Ping, E-mail:


    MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at post-transcriptional level. miRNA dysregulation plays a causal role in cancer progression. In this study, miR-208-3p was highly expressed and directly repressed ARID2 expression. As a result, ARID2 expression in hepatocellular carcinoma (HCC) was decreased. In vitro, miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. In vivo test results revealed that miR-208-3p down-regulation inhibited HCC tumorigenesis in Hep3B cells. Moreover, ARID2 was possibly a downstream element of transforming growth factor beta1 (TGFβ1)/miR-208-3p/ARID2 regulatory pathway. These findings suggested that miR-208-3p up-regulation is associated with HCC cell progression and may provide a new target for liver cancer treatment. - Highlights: • miR-208-3p was highly expressed and directly repressed the expression of ARID2 in HCC. • miR-208-3p contributed to HCC cell progression both in vitro and in vivo. • Over-expression of ARID2 inhibited the HCC cell proliferation and invasion. • Restoration of ARID2 partly reversed the the effect of miR-208-3p down-regulation on HCC cells. • Newly regulatory pathway: miR-208-3p mediated the repression of ARID2 by TGFβ1 in HCC cells.

  8. EuCo2P2 : A model molecular-field helical Heisenberg antiferromagnet

    Sangeetha, N. S.; Cuervo-Reyes, Eduardo; Pandey, Abhishek; Johnston, D. C.


    The metallic compound EuCo2P2 with the body-centered tetragonal ThCr2Si2 structure containing Eu spins-7/2 was previously shown from single-crystal neutron diffraction measurements to exhibit a helical antiferromagnetic (AFM) structure below TN=66.5 K with the helix axis along the c axis and with the ordered moments aligned within the a b plane. Here we report crystallography, electrical resistivity, heat capacity, magnetization, and magnetic susceptibility measurements on single crystals of this compound. We demonstrate that EuCo2P2 is a model molecular-field helical Heisenberg antiferromagnet from comparisons of the anisotropic magnetic susceptibility χ , high-field magnetization, and magnetic heat capacity of EuCo2P2 single crystals at temperature T ≤TN with the predictions of our recent formulation of molecular-field theory. Values of the Heisenberg exchange interactions between the Eu spins are derived from the data. The low-T magnetic heat capacity ˜T3 arising from spin-wave excitations with no anisotropy gap is calculated and found to be comparable to the lattice heat capacity. The density of states at the Fermi energy of EuCo2P2 and the related compound BaCo2P2 are found from the heat capacity data to be large, 10 and 16 states/eV per formula unit for EuCo2P2 and BaCo2P2 , respectively. These values are enhanced by a factor of ˜2.5 above those found from DFT electronic structure calculations for the two compounds. The calculations also find ferromagnetic Eu-Eu exchange interactions within the a b plane and AFM interactions between Eu spins in nearest- and next-nearest planes, in agreement with the MFT analysis of χa b(T ≤TN) .

  9. Measurement of Integrated Stokes Parameters for He 3 3p State Excited by Spin-Polarized Electrons

    DING Hai-Bing; PANG Wen-Ning; LIU Yi-Bao; SHANG Ren-Cheng


    @@ Integrated Stokes parameters Pi (i = 1, 2, 3) for the He 3 3p → 2 3S1 (388.9nm) transition after excitation from the ground state to the 3 3 P state by a transversely spin-polarized electron beam are measured in near threshold energy region. The experimental results are presented. The linear-polarization P2 are consistent with zero over the incident energy range, providing evidence for the LS coupling mechanism of the 3 3P state. The measured circular polarization P3 are non-zero, indicating strong electron-electron exchange effects in the spin-polarized electron-atom collision process.

  10. Microwave Dielectric Properties of A2P2O7 (A = Ca, Sr, Ba; Mg, Zn, Mn)

    Bian, Jian-jiang; Kim, Dong-Wan; Hong, Kug Sun


    Microwave dielectric properties of A2P2O7 (A = Ca, Sr, Ba, Mn, Mg, Ba) ceramic materials were investigated by a network analyzer at the frequency of 10 GHz. It was found that A2P2O7 ceramics could be sintered at relatively lower temperature below 1150°C, although the thortveitite type series, Mn2P2O7, α-Mg2P2O7 and α-Zn2P2O7 with smaller ionic radii of A cations were hard to sinter to full density. The dielectric constant of A2P2O7 is lower than 10. The Q× f value increased according to the sequence of δ-Ba2P2O7, α-Sr2P2O7 and β-Ca2P2O7 in dichromatic type series, and the sequence of Mn2P2O7, α-Mg2P2O7 and α-Zn2P2O7 in thortveitite type series, respectively. The temperature coefficient of resonant frequency τf for all samples exhibits negative value. Larger τf for α-Zn2P2O7, α-Mg2P2O7 and δ-Ba2P2O7 is mainly due to their reversible phase transformations. The microwave dielectric properties were discussed from the point view of bond valence.

  11. Functional expression of pig renal organic anion transporter 3 (pOAT3).

    Hagos, Yohannes; Braun, Isabella M; Krick, Wolfgang; Burckhardt, Gerhard; Bahn, Andrew


    With the cloning of pig renal organic anion transporter 1 (pOAT1) (Biochimie 84 (2002) 1219) we set up a model system for comparative studies of cloned and natively isolated membrane located transport proteins. Meanwhile, another transport protein involved in p-aminohippurate (PAH) uptake on the basolateral side of the proximal tubule cells was identified, designated organic anion transporter 3 (OAT3). To explore the contribution of pOAT1 to the PAH clearance in comparison to OAT3, it was the aim of this study to extend our model by cloning of the pig ortholog of OAT3. Sequence comparisons of human organic anion transporter 3 (hOAT3) with the expressed sequence tag (EST) database revealed a clone and partial sequence of the pig renal organic anion transporter 3 (pOAT3) ortholog. Sequencing of the entire open reading frame resulted in a protein of 543 amino acid residues encoded by 1632 base pairs (EMBL Acc. No. AJ587003). It showed high homologies of 81%, 80%, 76%, and 77% to the human, rabbit, rat, and mouse OAT3, respectively. A functional characterization of pOAT3 in Xenopus laevis oocytes yielded an apparent Km (Kt) for [3H]estrone sulfate of 7.8 +/- 1.3 microM. Moreover, pOAT3 mediated [3H]estrone sulfate uptake was almost abolished by 0.5 mM of glutarate, dehydroepiandosterone sulfate, or probenecid consistent with the hallmarks of OAT3 function.

  12. Precision frequency measurement of 1S0-3P1 intercombination lines of Sr isotopes

    Liu, Hui; Gao, Feng; Ye-Bing, Wang; Xiao, Tian; Jie, Ren; Ben-Quan, Lu; Qin-Fang, Xu; Yu-Lin, Xie; Hong, Chang


    We report on frequency measurement of the intercombination (5s2)1S0-(5s5p)3P1 transition of the four natural isotopes of strontium, including 88Sr (82.58%), 87Sr (7.0%), 86Sr (9.86%), and 84Sr (0.56%). A narrow-linewidth laser that is locked to an ultra-low expansion (ULE) optical cavity with a finesse of 12000 is evaluated at a linewidth of 200 Hz with a fractional frequency drift of 2.8×10-13 at an integration time of 1 s. The fluorescence collector and detector are specially designed, based on a thermal atomic beam. Using a double-pass acousto-optic modulator (AOM) combined with a fiber and laser power stabilization configuration to detune the laser frequency enables high signal-to-noise ratios and precision saturated spectra to be obtained for the six transition lines, which allows us to determine the transition frequency precisely. The optical frequency is measured using an optical frequency synthesizer referenced to an H maser. Both the statistical values and the final values, including the corrections and uncertainties, are derived for a comparison with the values given in other works. Project supported by the National Natural Science Foundation of China (Grant No. 61127901) and the Key Project of the Chinese Academy of Sciences (Grant No. KJZD-EW-W02).

  13. Join the CERN ISEF special award winners | 16 June - 3 p.m.


    Come and join the CERN ISEF special award winners at their lightning talks session on 16 June at 3.00 p.m. in the main auditorium.   The 2016 Intel ISEF CERN special award winners on stage with the selection committee on 17 May 2016 in Phoenix, Arizona, USA. (Picture: Society for Science and the Public) Between 11 and 17 June 2016, the ten finalists of the Intel International Science and Engineering Fair (ISEF) who won the CERN Special Award, will visit CERN to partake in various educational lectures. ISEF is the world's largest international pre-college science competition, with approximately 1,700 high school students from more than 75 countries taking part. They will present their projects in short 5 minutes lightning talks' sessions at the main auditorium on Thursday 16 June at 3 p.m. The award winners would be also very happy to have a chance to interact and discuss with you af...

  14. Dynamics of the gas-liquid interfacial reaction of O(3P) atoms with hydrocarbons

    Kelso, Hailey; Köhler, Sven P. K.; Henderson, David A.; McKendrick, Kenneth G.


    We describe an experimental approach to the determination of the nascent internal state distribution of gas-phase products of a gas-liquid interfacial reaction. The system chosen for study is O(3P) atoms with the surface of liquid deuterated squalane, a partially branched long-chain saturated hydrocarbon, C30D62. The nascent OD products are detected by laser-induced fluorescence. Both OD (v'=0) and (v'=1) were observed in significant yield. The rotational distributions in both vibrational levels are essentially the same, and are characteristic of a Boltzmann distribution at a temperature close to that of the liquid surface. This contrasts with the distributions in the corresponding homogeneous gas-phase reactions. We propose a preliminary interpretation in terms of a dominant trapping-desorption mechanism, in which the OD molecules are retained at the surface sufficiently long to cause rotational equilibration but not complete vibrational relaxation. The significant yield of vibrationally excited OD also suggests that the surface is not composed entirely of -CD3 endgroups, but that secondary and/or tertiary units along the backbone are exposed.

  15. Theoretical investigation of hyperthermal reactions at the gas-liquid interface: O (3P) and squalane.

    Kim, Dongwook; Schatz, George C


    Hyperthermal collisions (5 eV) of ground-state atomic oxygen [O ((3)P)] with a liquid-saturated hydrocarbon, squalane (C(30)H(62)), have been studied using QM/MM hybrid "on-the-fly" direct dynamics. The surface structure of the liquid squalane is obtained from a classical molecular dynamics simulation using the OPLS-AA force field. The MSINDO semiempirical Hamiltonian is combined with OPLS-AA for the QM/MM calculations. In order to achieve a more consistent and efficient simulation of the collisions, we implemented a dynamic partitioning of the QM and MM atoms in which atoms are assigned to QM or MM regions based on their proximity to "seed" (open-shell) atoms that determine where bond making/breaking can occur. In addition, the number of seed atoms is allowed to increase or decrease as time evolves so that multiple reactive events can be described. The results show that H abstraction is the most important process for all incident angles, with H elimination, double H abstraction, and C-C bond cleavage also being important. A number of properties of these reactive channels, as well as inelastic nonreactive scattering, are investigated, including angular and translational energy distributions, the effect of incident collision angle, variation with depth of the reactive event within the liquid, with the reaction site on the hydrocarbon, and the effect of dynamics before and after reaction (direct reaction versus trapping reaction-desorption).

  16. Dynamics of interfacial reactions between O(3 P) atoms and long-chain liquid hydrocarbons

    Allan, Mhairi; Bagot, Paul A. J.; Köhler, Sven P. K.; Reed, Stewart K.; Westacott, Robin E.; Costen, Matthew L.; McKendrick, Kenneth G.


    Recent progress that has been made towards understanding the dynamics of collisions at the gas-liquid interface is summarized briefly. We describe in this context a promising new approach to the experimental study of gas-liquid interfacial reactions that we have introduced. This is based on laser-photolytic production of reactive gas-phase atoms above the liquid surface and laser-spectroscopic probing of the resulting nascent products. This technique is illustrated for reaction of O(3P) atoms at the surface of the long-chain liquid hydrocarbon squalane (2,6,10,15,19,23-hexamethyltetracosane). Laser-induced fluorescence detection of the nascent OH has revealed mechanistically diagnostic correlations between its internal and translational energy distributions. Vibrationally excited OH molecules are able to escape the surface. At least two contributions to the product rotational distributions are identified, confirming and extending previous hypotheses of the participation of both direct and trapping-desorption mechanisms. We speculate briefly on future experimental and theoretical developments that might be necessary to address the many currently unanswered mechanistic questions for this, and other, classes of gas-liquid interfacial reaction.

  17. Excitons into one-axis crystals of zinc phosphide (Zn3P2

    D.M. Stepanchikov


    Full Text Available Theoretical study of excitons spectra is offered in this report as for Zn3P2 crystals. Spectra are got in the zero approach of the theory of perturbations with consideration of both the anisotropy of the dispersion law and the selection rules. The existence of two exciton series was found, which corresponds to two valence bands (hh, lh and the conductivity band (c. It is noteworthy that anisotropy of the dispersion law plus the existence of crystalline packets (layers normal to the main optical axis, both will permit the consideration of two-dimensional excitons too. The high temperature displaying of these 2D-exciton effects is not eliminated even into bulk crystals. The calculated values of the binding energies as well as the oscillator's strength for the optical transitions are given for a volume (3D and for two-dimensional (2D excitons. The model of energy exciton transitions and four-level scheme of stimulated exciton radiation for receiving laser effect are offered.

  18. Using the Reputation Score Management for Constructing Fair P2P File Sharing System

    Jun Han


    Full Text Available This paper has used the reputation score management for constructing a fair P2P file sharing system, the system design principle is simple and easy to realize, and every node entering into the P2P network obtains a certain reputation score, and obtains the corresponding resources reward according to the score. This paper has described the fair sharing strategies facing node network bandwidth and TTL, and these strategies can be used independently or be combined with other reputation score managements of P2P network. These two strategies have been discussed in the specific reputation score management system of P2P network Eigen Trust, and the test results indicate that: compared with a common P2P network, the fair sharing strategies of this paper have faster file download speed and can decrease the network message communication amount during the process looking for resources. It can also be combined with another reputation management system. it is simple and easy to be realized, its main purposes are to fairly share network bandwidth and to decrease information communication volume, and it can suppress the free riding behavior to some extent.

  19. Monitoring autophagic flux using Ref(2)P, the Drosophila p62 ortholog.

    DeVorkin, Lindsay; Gorski, Sharon M


    Human p62, also known as Sequestome-1 (SQSTM1), is a multifunctional scaffold protein that contains many domains, including a Phox/Bem1P (PB1) multimerization domain, an ubiquitin-associated (UBA) domain, and a light chain 3 (LC3) recognition sequence. p62 binds ubiquitinated proteins and targets them for degradation by the proteasome. In addition, p62 directly binds LC3; this may serve as a mechanism to deliver ubiquitinated proteins for degradation by autophagy. During this process, p62 itself is degraded. The inhibition of autophagy leads to the accumulation of p62, indicating that it can be used as a marker of autophagic flux. Ref(2)P (refractory to sigma P), the Drosophila ortholog of p62, is also required for the formation of ubiquitinated protein aggregates. Ref(2)P contains a putative LC3-interacting region, and genetic inhibition of autophagy in Drosophila leads to the accumulation of Ref(2)P protein levels. Thus, like p62, Ref(2)P may serve as a marker of autophagic flux. Here we provide two procedures to examine Ref(2)P protein levels in Drosophila ovaries.

  20. Large-Scale Mini-Magnetosphere Plasma Propulsion (M2P2) Experiments

    Winglee, R. M.; Slough, J.; Ziemba, T.; Euripides, P.; Adrian, M. L.; Gallagher, D.; Craven, P.; Tomlinson, W.; Cravens, J.; Burch, J.; Whitaker, Ann F. (Technical Monitor)


    Mini-Magnetosphere Plasma Propulsion (M2P2) is an innovative plasma propulsion system that has the potential to propel spacecraft at unprecedented speeds of 50 to 80 km per second with a low-power requirement of approx. 1 kW per 100 kg of payload and approx. 1 kg of neutral gas [fuel] consumption per day of acceleration. Acceleration periods from several days to a few months are envisioned. High specific impulse and efficiency are achieved through coupling of the spacecraft to the 400 km per second solar wind through an artificial magnetosphere. The mini-magnetosphere or inflated magnetic bubble is produced by the injection of cold dense plasma into a spacecraft-generated magnetic field envelope. Magnetic bubble inflation is driven by electromagnetic processes thereby avoiding the material and deployment problems faced by mechanical solar sail designs, Here, we present the theoretical design of M2P2 as well as initial results from experimental testing of an M2P2 prototype demonstrating: 1) inflation of the dipole magnetic field geometry through the internal injection of cold plasma; and 2) deflection of and artificial solar wind by the prototype M2P2 system. In addition, we present plans for direct laboratory measurement of thrust imparted to a prototype M2P2 by an artificial solar wind during the summer of 2001.

  1. An Efficient Architecture for Information Retrieval in P2P Context Using Hypergraph

    Ismail, Anis; Durand, Nicolas; Hajjar, Mohammad


    Peer-to-peer (P2P) Data-sharing systems now generate a significant portion of Internet traffic. P2P systems have emerged as an accepted way to share enormous volumes of data. Needs for widely distributed information systems supporting virtual organizations have given rise to a new category of P2P systems called schema-based. In such systems each peer is a database management system in itself, ex-posing its own schema. In such a setting, the main objective is the efficient search across peer databases by processing each incoming query without overly consuming bandwidth. The usability of these systems depends on successful techniques to find and retrieve data; however, efficient and effective routing of content-based queries is an emerging problem in P2P networks. This work was attended as an attempt to motivate the use of mining algorithms in the P2P context may improve the significantly the efficiency of such methods. Our proposed method based respectively on combination of clustering with hypergraphs. We use...

  2. 2P/Encke, the Taurid complex NEOs and the Maribo and Sutter's Mill meteorites

    Tubiana, C; Michelsen, R; Haack, H; Boehnhardt, H; Fitzsimmons, A; Williams, I P


    The Taurid meteoroid stream has long been linked with 2P/Encke owing to a good match of their orbital elements, even though the comet's activity is not strong enough to explain the number of observed meteors. Various small NEOs have been discovered with orbits that can be linked to 2P and the Taurid meteoroid stream. Maribo and Sutter's Mill are CM type carbonaceous chondrites that fell in Denmark on Jan 17, 2009 and Apr 22, 2012, respectively. Their pre-atmospheric orbits place them in the middle of the Taurid meteoroid stream, which raises the intriguing possibility that comet 2P could be the parent body of CM chondrites. To investigate whether a relationship between comet 2P, the Taurid complex associated NEOs, and CM chondrites exists, we performed photometric and spectroscopic studies of these objects in the visible wavelength range. We observed 2P and 10 NEOs on Aug 2, 2011 with FORS at the VLT. Images in the R filter, used to investigate the possible presence of cometary activity around the nucleus of ...

  3. P2P Streaming Traffic Classification in High-Speed Networks

    Chen Luying; Cong Rong; Yang Jie; Yu Hua


    The growing P2P streaming traffic brings a variety of problems and challenges to ISP networks and service providers.A P2P streaming traffic classification method based on sampling technology is presented in this paper.By analyzing traffic statistical features and network behavior of P2P streaming,a group of flow characteristics were found,which can make P2P streaming more recognizable among other applications.Attributes from Netflow and those proposed by us are compared in terms of classification accuracy,and so are the results of different sampling rates.It is proved that the unified classification model with the proposed attributes can identify P2P streaming quickly and efficiently in the online system.Even with 1∶50 sampling rate,the recognition accuracy can be higher than 94%.Moreover,we have evaluated the CPU resources,storage capacity and time consumption before and after the sampling,it is shown that the classification model after the sampling can significantly reduce the resource requirements with the same recognition accuracy.

  4. Synthesis, characterization and photocatalytic performance of PbS/Ni2P flowers

    Liu, Shuling; Han, Lefang; Liu, Hui


    Flower-like PbS/Ni2P composites were synthesized by a facile two-step chemical route. The morphology and structure of the resulting composites were investigated by SEM and TEM images and XRD spectra, respectively. The results showed that the as-obtained composites were composed of the cubic PbS flowers and hexagonal Ni2P nanoparticles, and Ni2P nanoparticles coated on the surfaces of flower-like PbS microstructure. It was found that changing the molar ratio to 3:1, flower-like PbS/Ni2P composites have been successfully synthesized by using cationic cetyltrimethylammonium bromide(CTAB) and anionic sodium dodecyl sulfate (SDS) as template. Furthermore, using methylene blue(MB) as a model organic pollutant, the photocatalytic degradation experiments indicated that the as-prepared composites showed enhanced photocatalytic degradation activity for methylene blue(75%) which is as higher as that of the only flower-like PbS (16.6%) and only Ni2P nanoparticles (44.8%) at the same time. This work may be expected to find its potential application in water pollution treatment.


    Liu Hui; Huang Yongfeng; Li Xing


    The rapid development of Internet has led to the explosion of information sharing, and how to supervise the sharing is a main research topic on current Internet. Aiming at the disadvantage that the current Peer-to-Peer (P2P) is hard to manage and control, this paper presents a Session Initial Protocol (SIP)-based P2P network of three-level architecture. SIP middleware is introduced to the middle level of the three-layer architecture. By the connection function of the SIP signaling, the P2P transmission on media-level can be controlled. Using SIP's register and authentication function, the manage layer can manage the whole P2P network. Based on the aforementioned architecture, this paper investigates the grouping strategy on a live broadcast application in P2P network. Combined with the function of SIP register, the paper works on several grouping strategies, sets up models to manage users by grouping them, presents a weight-based K-means IP address grouping algorithm, and realizes it. The experiment shows that the grouping strategy presented in this paper can solve the problem of group sharing of network resource, and can realize the efficient-sharing, reasonable-distributing of network resource.

  6. Secure mobile agent for telemedicine based on P2P networks.

    Hsu, Wen-Shin; Pan, Jiann-I


    Exploring intelligent mobile agent (MA) technology for assisting medical services or transmitting personal patient-health information in telemedicine applications has been widely investigated. Conversely, peer-to-peer (P2P) networking has become one of the most popular applications used in the Internet because of its benefits for easy-to-manage resources and because it balances workloads. Therefore, constructing an agent-based telemedicine platform based on P2P networking architecture is necessary. The main purpose of this paper is to construct a safe agent-based telemedicine that based on P2P networking architecture. Two themes are addressed in this paper: (a) the P2P network architecture for an agent-based telemedicine service, and (b) the security mechanisms for the proposed telemedicine networking architecture. When an MA contains patient information and migrates from one host to another through the Internet, it can be attacked by other software agents or agent platforms that can illegally access patient information. The proposed P2P network architecture is based on the JXTA protocol and provides two types of telemedicine service models: the predictable service model and unpredictable service model. This architecture employs a two-layer safety mechanism for MAs (i.e., time-limited black boxes and RSA undetachable signature technologies), to provide a secure solution for agent-based telemedicine services.

  7. Information Exchange rather than Topology Awareness: Cooperation between P2P Overlay and Traffic Engineering

    Jia Zhao


    Full Text Available Solutions to the routing strategic conflict between noncooperative P2P overlay and ISP underlay go separate ways: hyperselfishness and cooperation. Unpredictable (possibly adverse impact of the hyperselfish topology awareness, which is adopted in both overlay routing and traffic engineering, has not been sufficiently studied in the literature. Topology-related information exchange in a cooperatively efficient way should be highlighted to alleviate the cross-layer conflict. In this paper, we first illustrate the hyperselfish weakness with two dynamic noncooperative game models in which hyperselfish overlay or underlay has to accept a suboptimal profit. Then we build a synergistic cost-saving (SC game model to reduce the negative effects of noncooperation. In the SC model, through information exchange, that is, the classified path-delay metrics for P2P overlay and peer locations for underlay, P2P overlay selects proximity as well as saving traffic transit cost for underlay, and ISP underlay adjusts routing to optimize network cost as well as indicating short delay paths for P2P. Simulations based on the real and generated topologies validate cost improvement by SC model and find a proper remote threshold value to limit P2P traffic from remote area, cross-AS, or cross-ISP.

  8. 共沉默miR-221-3p/222-3p表达抑制骨髓间充质干细胞增殖及促成软骨分化%Down-regulation of miR-221-3p/222-3p inhibits cell proliferation and promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells

    闫继红; 周德山; 杨姝; 孙海梅; 曹丹丹; 张秀英; 季凤清; 郭多; 吴波; 孙婷怡


    BACKGROUND:The use of mesenchymal stem cels in the field of tissue engineering for osteoarticular injury repair is a very promising tool since these cels are readily expandable and able to differentiate into chondrocytes. Abundant evidence suggests that microRNAs play critical roles in chondrogenic differentiation of mesenchymal stem cels. OBJECTIVE:To observe the chondrogenic effect of human bone marrow mesenchymal stem cels transfected with lentiviral vectors bearing miR-221-3p/222-3p inhibition, thereby provding new strategies for cartilage injury. METHODS: miRNA microarray technology was applied to detect microRNAs expression profiles at three different stages of chondrogenic differentiation induction after transforming growth factor-β3 treatment and verified by real-time fluorescence quantitative PCR (RT-qPCR). Human bone marrow mesenchymal stem cels were infected with lentivirus bearing miR-221-3p/222-3p inhibition. After co-suppressing the expression of miR-221/222-3p, cel counting kit-8 was used to determine the cel proliferation, the differentiation of bone marrow mesenchymal stem cels towards chondrocytes was verified by type II colagen protein expression through immunohistochemistry and glycosaminoglycan accumulation was also elevated by sarranine O staining. RT-PCR was used to detect type II colagen and aggrecan mRNA expression at 21 days of chondrogenic induction. RESULTS AND CONCLUSION: The expression of miR-221-3p/222-3p was inhibited after Lv-miR221-3p/222-3p inhibition co-transfected into bone marrow mesenchymal stem cels. microRNA microarray and RT-qPCR results showed that the expression of miR-221-3p/222-3p was declined significantly at the anaphase of chondrogenic differentiation. The expression levels of chondrogenic markers, Aggrecan and type II colagen were significantly increased in the miR-221-3p/222-3p inhibition group and cel proliferation was also inhibited significantly compared with non-transduced cels or transduced with the empty

  9. Bonding and site preferences in the new quasi-binary Zr{sub 2.7}Hf{sub 11.3}P{sub 9}

    Kleinke, H.; Franzen, H.F. [Ames Lab., IA (United States)


    The new quasi-binary Zr{sub 2.7}Hf{sub 11.3}P{sub 9} was synthesized by arc-melting of Zr, Hf, Co, and HfP in a ratio corresponding to the initial composition Zr{sub 2.25}Hf{sub 6.75}Co{sub 2}P{sub 4}. Zr{sub 2.7}Hf{sub 11.3}P{sub 9} crystallizes in the Zr{sub 14}P{sub 9} structure type, which is unknown in the binary Hf/P system. the ideal orthorhomibic lattice dimensions (space group Pnnm (No. 58), Z = 4) were refined to a = 16.640(7) {angstrom}, b = 27.40(2) {angstrom}, c = 3.619(1) {angstrom}, V = 1650(2) {angstrom}{sup 3}. The structure consists of three-dimensional condensed one-, two-, and three-capped trigonal (Zr, Hf){sub 6}P prisms, occurring with numerous short M-M bonds (M = Zr, Hf). Each of the 15 metal sites is statistically occupied by a mixture of Zr and Hf, which varies significantly from site to site. The Hf/Zr ratio in a given site depends on the M-M and M-P interactions. The systematic increase of this ratio with increasing total bond order, as evaluated via Mulliken overlap populations and Pauling bond orders, can be understood based on the trend that Hf forms stronger M-M and M-P bonds than Zr. As expected for a metal-rich phosphide, band structure calculations for the hypothetical Hf{sub 14}P{sub 9} carried out with the extended Hueckel approximation result in a significant density of states at the Fermi level.

  10. External lipid PI3P mediates entry of eukaryotic pathogen effectors into plant and animal host cells.

    Kale, Shiv D; Gu, Biao; Capelluto, Daniel G S; Dou, Daolong; Feldman, Emily; Rumore, Amanda; Arredondo, Felipe D; Hanlon, Regina; Fudal, Isabelle; Rouxel, Thierry; Lawrence, Christopher B; Shan, Weixing; Tyler, Brett M


    Pathogens of plants and animals produce effector proteins that are transferred into the cytoplasm of host cells to suppress host defenses. One type of plant pathogens, oomycetes, produces effector proteins with N-terminal RXLR and dEER motifs that enable entry into host cells. We show here that effectors of another pathogen type, fungi, contain functional variants of the RXLR motif, and that the oomycete and fungal RXLR motifs enable binding to the phospholipid, phosphatidylinositol-3-phosphate (PI3P). We find that PI3P is abundant on the outer surface of plant cell plasma membranes and, furthermore, on some animal cells. All effectors could also enter human cells, suggesting that PI3P-mediated effector entry may be very widespread in plant, animal and human pathogenesis. Entry into both plant and animal cells involves lipid raft-mediated endocytosis. Blocking PI3P binding inhibited effector entry, suggesting new therapeutic avenues.

  11. 基于 P2P 网络的 Bitcoin 虚拟货币分析%Bitcoin Virtual Currency Analysis Based on P2P

    石苗; 李宥谋


    Bitcoin 是一种基于 P2P 网络的匿名数字货币,论文从网络虚拟货币的发展现状出发,从技术层面详细分析Bitcoin 形成相关技术原理及其交易过程和验证机制,肯定了其历史性和革命性。同时分析 Bitcoin 存在的各方面问题,探究其在网络虚拟货币统一方面的重要借鉴意义。%Bitcoin is an anonymous digital cash based on P2P network .In this paper ,starting from the development present situation of the network virtual currency ,detailed analysis formation related technical principles and transaction process and the authentication mechanism from the technical level of Bitcoin ,affirmed its historical and revolutionary .At the same time ,aspects of problems of Bitcoin are analyzed ,its importance in the network virtual currency unification is explored .

  12. Theoretical study on the two-band degenerate-gaps superconductors: Application to SrPt3P

    Huang, Hai; Hou, Li-Chao; Zhao, Bin-Peng


    We study the magnetic properties of two-band degenerate-gaps superconductors with two-band isotropic Ginzburg-Landau theory. The exact solutions of upper critical field and London penetration depth are obtained, and the calculations reproduce the experimental data of the recently observed superconducting crystal SrPt3P in a broad temperature range. It directly underlies that SrPt3P is a multi-band superconductor with equal gaps in two Fermi surface sheets.

  13. The Involvement of miR-29b-3p in Arterial Calcification by Targeting Matrix Metalloproteinase-2

    Jiang, Wenhong; Zhang, Zhanman; Yang, Han; Lin, Qiuning; Han, Chuangye


    Vascular calcification is a risk predictor and common pathological change in cardiovascular diseases that are associated with elastin degradation and phenotypic transformation of vascular smooth muscle cells via gelatinase matrix metalloproteinase-2 (MMP2). However, the mechanisms involved in this process remain unclear. In this study, we investigated the relationships between miR-29b-3p and MMP2, to confirm miR-29b-3p-mediated MMP2 expression at the posttranscriptional level in arterial calcification. In male Sprague Dawley rats, arterial calcification was induced by subcutaneous injection of a toxic dose of cholecalciferol. In vivo, the quantitative real-time polymerase chain reaction (qRT-PCR) showed that MMP2 expression was upregulated in calcified arterial tissues, and miR-29b-3p expression was downregulated. There was a negative correlation between MMP2 mRNA expression and miR-29b-3p levels (P = 0.0014, R2 = 0.481). Western blotting showed that MMP2 expression was significantly increased in rats treated with cholecalciferol. In vitro, overexpression of miR-29b-3p led to decreased MMP2 expression in rat vascular smooth muscle cells, while downregulation of miR-29b-3p expression led to increased MMP2 expression. Moreover, the luciferase reporter assay confirmed that MMP2 is the direct target of miR-29b-3p. Together, our results demonstrated that a role of miR-29b-3p in vascular calcification involves targeting MMP2. PMID:28164126

  14. The Involvement of miR-29b-3p in Arterial Calcification by Targeting Matrix Metalloproteinase-2

    Wenhong Jiang


    Full Text Available Vascular calcification is a risk predictor and common pathological change in cardiovascular diseases that are associated with elastin degradation and phenotypic transformation of vascular smooth muscle cells via gelatinase matrix metalloproteinase-2 (MMP2. However, the mechanisms involved in this process remain unclear. In this study, we investigated the relationships between miR-29b-3p and MMP2, to confirm miR-29b-3p-mediated MMP2 expression at the posttranscriptional level in arterial calcification. In male Sprague Dawley rats, arterial calcification was induced by subcutaneous injection of a toxic dose of cholecalciferol. In vivo, the quantitative real-time polymerase chain reaction (qRT-PCR showed that MMP2 expression was upregulated in calcified arterial tissues, and miR-29b-3p expression was downregulated. There was a negative correlation between MMP2 mRNA expression and miR-29b-3p levels (P=0.0014, R2=0.481. Western blotting showed that MMP2 expression was significantly increased in rats treated with cholecalciferol. In vitro, overexpression of miR-29b-3p led to decreased MMP2 expression in rat vascular smooth muscle cells, while downregulation of miR-29b-3p expression led to increased MMP2 expression. Moreover, the luciferase reporter assay confirmed that MMP2 is the direct target of miR-29b-3p. Together, our results demonstrated that a role of miR-29b-3p in vascular calcification involves targeting MMP2.

  15. Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis.

    Dostál, Jiří; Pecina, Adam; Hrušková-Heidingsfeldová, Olga; Marečková, Lucie; Pichová, Iva; Řezáčová, Pavlina; Lepšík, Martin; Brynda, Jiří


    The virulence of the Candida pathogens is enhanced by the production of secreted aspartic proteases, which therefore represent possible targets for drug design. Here, the crystal structure of the secreted aspartic protease Sapp2p from Candida parapsilosis was determined. Sapp2p was isolated from its natural source and crystallized in complex with pepstatin A, a classical aspartic protease inhibitor. The atomic resolution of 0.83 Å allowed the protonation states of the active-site residues to be inferred. A detailed comparison of the structure of Sapp2p with the structure of Sapp1p, the most abundant C. parapsilosis secreted aspartic protease, was performed. The analysis, which included advanced quantum-chemical interaction-energy calculations, uncovered molecular details that allowed the experimentally observed equipotent inhibition of both isoenzymes by pepstatin A to be rationalized.

  16. P2PRPIPS: A P2P and Reverse Proxy Based Web Intrusion Protection System

    Qian He


    Full Text Available In order to protect web sites with various program languages and high throughput efficiently, a web Intrusion Protection System (IPS based on P2P and reverse proxy architecture was designed and implemented. The P2P based web intrusion protection system has multi web firewall nodes and nodes with same program cooperate with each other under P2P architecture. Some nodes work as net flow allocator and some work as detector and they can convert to each other according to the requirements dynamically. The WAF program has the characteristics of session keeping and load balancing and it can detect messages by using expert library and many plug-in components. The technology of reverse proxy is used for response the web request. Experiments show that the system can effectively prevent attacks form application layer. It is proved more efficient and stable than single node.

  17. Kinetic-arrest-induced phase coexistence and metastability in (Mn,Fe ) 2(P ,Si )

    Miao, X. F.; Mitsui, Y.; Dugulan, A. Iulian; Caron, L.; Thang, N. V.; Manuel, P.; Koyama, K.; Takahashi, K.; van Dijk, N. H.; Brück, E.


    Neutron diffraction, Mössbauer spectroscopy, magnetometry, and in-field x-ray diffraction are employed to investigate the magnetoelastic phase transition in hexagonal (Mn,Fe ) 2(P ,Si ) compounds. (Mn,Fe ) 2(P ,Si ) compounds undergo for certain compositions a second-order paramagnetic (PM) to a spin-density-wave (SDW) phase transition before further transforming into a ferromagnetic (FM) phase via a first-order phase transition. The SDW-FM transition can be kinetically arrested, causing the coexistence of FM and untransformed SDW phases at low temperatures. Our in-field x-ray diffraction and magnetic relaxation measurements clearly reveal the metastability of the untransformed SDW phase. This unusual magnetic configuration originates from the strong magnetoelastic coupling and the mixed magnetism in hexagonal (Mn,Fe ) 2(P ,Si ) compounds.

  18. A P2P Botnet Virus Detection System Based on Data-Mining Algorithms

    Wernhuar Tarng


    Full Text Available A P2P botnet virus detection system based on data-mining algorithms is proposed in this study to detect theinfected computers quickly using Bayes Classifier and Neural Network (NN Classifier. The system candetect P2P botnet viruses in the early stage of infection and report to network managers to avoid furtherinfection. The system adopts real-time flow identification techniques to detect traffic flows produced by P2Papplication programs and botnet viruses by comparing with the known flow patterns in the database. Aftertrained by adjusting the system parameters using test samples, the experimental results show that theaccuracy of Bayes Classifier is 95.78% and that of NN Classifier is 98.71% in detecting P2P botnet virusesand suspected flows to achieve the goal of infection control in a short time.

  19. Public Key Cryptosystem Approach for P2P Botnet Detection and Prevention

    Anas Aliyu Usman


    Full Text Available Distributed (P2P botnets have as of late been received by botmasters for their versatility against take-down endeavors. Other than being harder to bring down, p2p botnets tend to be stealthier in the way they perform vindictive exercises, making current discovery approaches ineffectual. In this paper, we simulate our proposal by detecting a gray hole attack in an Ad Hoc network using NS2.The detected malicious node is listed in a black hole list and notices all other nodes in the network to stop communicating with them. Our botnet location framework has been equipped for identifying stealthy P2P botnets (Gray Hole nodes and can reduce packet loss caused by malicious nodes and have a better packet delivery ratio (PDR within less period of time.

  20. The N2-P3 complex of the evoked potential and human performance

    Odonnell, Brian F.; Cohen, Ronald A.


    The N2-P3 complex and other endogenous components of human evoked potential provide a set of tools for the investigation of human perceptual and cognitive processes. These multidimensional measures of central nervous system bioelectrical activity respond to a variety of environmental and internal factors which have been experimentally characterized. Their application to the analysis of human performance in naturalistic task environments is just beginning. Converging evidence suggests that the N2-P3 complex reflects processes of stimulus evaluation, perceptual resource allocation, and decision making that proceed in parallel, rather than in series, with response generation. Utilization of these EP components may provide insights into the central nervous system mechanisms modulating task performance unavailable from behavioral measures alone. The sensitivity of the N2-P3 complex to neuropathology, psychopathology, and pharmacological manipulation suggests that these components might provide sensitive markers for the effects of environmental stressors on the human central nervous system.

  1. The 2p-2h electromagnetic response in the quasielastic peak and beyond

    De Pace, A. E-mail:; Nardi, M.; Alberico, W.M.; Donnelly, T.W.; Molinari, A


    The contribution to the nuclear transverse response function R{sub T} arising from two particle-two hole (2p-2h) states excited through the action of electromagnetic meson exchange currents (MEC) is computed in a fully relativistic framework. The MEC considered are those carried by the pion and by {delta} degrees of freedom, the latter being viewed as a virtual nucleonic resonance. The calculation is performed in the relativistic Fermi gas model in which Lorentz covariance can be maintained. All 2p-2h many-body diagrams containing two pionic lines that contribute to R{sub T} are taken into account and the relative impact of the various components of the MEC on R{sub T} is addressed. The non-relativistic limit of the MEC contributions is also discussed and compared with the relativistic results to explore the role played by relativity in obtaining the 2p-2h nuclear response.

  2. Supporting K nearest neighbors query on high-dimensional data in P2P systems

    Mei LI; Wang-Chien LEE; Anand SIVASUBRAMANIAM; Jizhong ZHAO


    Peer-to-peer systems have been widely used for sharing and exchanging data and resources among numerous computer nodes.Various data objects identifiable with high dimensional feature vectors,such as text,images,genome sequences,are starting to leverage P2P technology.Most of the existing works have been focusing on queries on data objects with one or few attributes and thus are not applicable on high dimensional data objects.In this study,we investigate K nearest neighbors query (KNN)on high dimensional data objects in P2P systems.Efficient query algorithm and solutions that address various technical challenges raised by high dimensionality,such as search space resolution and incremental search space refinement,are proposed.An extensive simulation using both synthetic and real data sets demonstrates that our proposal efficiently supports KNN query on high dimensional data in P2P systems.

  3. High-pressure elastic properties of cubic Ir2P from ab initio calculations

    Sun, Xiao-Wei; Bioud, Nadhira; Fu, Zhi-Jian; Wei, Xiao-Ping; Song, Ting; Li, Zheng-Wei


    A study of the high-pressure elastic properties of new synthetic Ir2P in the anti-fluorite structure is conducted using ab initio calculations based on density functional theory. The elastic constants C11, C12 and C44 for the cubic Ir2P are obtained by the stress-strain method and the elastic stability calculations under pressure indicate that it is stable at least 100 GPa. Additionally, the electronic density of states, the aggregate elastic moduli, that is bulk modulus, shear modulus, and Young's modulus along with the Debye temperature, Poisson's ratio, and elastic anisotropy factor are all successfully obtained. Moreover, the pressure dependence of the longitudinal and shear wave velocities in three different directions [100], [110], and [111] for Ir2P are also predicted for the first time.

  4. Atypical features of a Ure2p glutathione transferase from Phanerochaete chrysosporium.

    Thuillier, Anne; Roret, Thomas; Favier, Frédérique; Gelhaye, Eric; Jacquot, Jean-Pierre; Didierjean, Claude; Morel-Rouhier, Mélanie


    Glutathione transferases (GSTs) are known to transfer glutathione onto small hydrophobic molecules in detoxification reactions. The GST Ure2pB1 from Phanerochaete chrysosporium exhibits atypical features, i.e. the presence of two glutathione binding sites and a high affinity towards oxidized glutathione. Moreover, PcUre2pB1 is able to efficiently deglutathionylate GS-phenacylacetophenone. Catalysis is not mediated by the cysteines of the protein but rather by the one of glutathione and an asparagine residue plays a key role in glutathione stabilization. Interestingly PcUre2pB1 interacts in vitro with a GST of the omega class. These properties are discussed in the physiological context of wood degrading fungi.

  5. 11th International Conference on P2P, Parallel, Grid, Cloud and Internet Computing

    Barolli, Leonard; Amato, Flora


    P2P, Grid, Cloud and Internet computing technologies have been very fast established as breakthrough paradigms for solving complex problems by enabling aggregation and sharing of an increasing variety of distributed computational resources at large scale. The aim of this volume is to provide latest research findings, innovative research results, methods and development techniques from both theoretical and practical perspectives related to P2P, Grid, Cloud and Internet computing as well as to reveal synergies among such large scale computing paradigms. This proceedings volume presents the results of the 11th International Conference on P2P, Parallel, Grid, Cloud And Internet Computing (3PGCIC-2016), held November 5-7, 2016, at Soonchunhyang University, Asan, Korea.

  6. Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100

    Víctor Toledano


    Full Text Available We show that galactomannan, a polysaccharide consisting of a mannose backbone with galactose side groups present on the cell wall of several fungi, induces a reprogramming of the inflammatory response in human macrophages through dectin-1 receptor. The nuclear factor kappa-light-chain-enhancer of activated B cells 2 (NFκB2/p100 was overexpressed after galactomannan challenge. Knocking down NFκB2/p100 using small interfering RNA (siRNA indicated that NFκB2/p100 expression is a crucial factor in the progression of the galactomannan-induced refractoriness. The data presented in this study could be used as a modulator of inflammatory response in clinical situations where refractory state is required.

  7. Analytical Modelling of Localized P2P Streaming Systems under NAT Consideration

    Mohammad Z. M. Masoud


    Full Text Available NAT has been design to work with Internet client-server structure. The emerged of Peer-to-Peer (P2Pnetworks and applications revealed the incompatibility between P2P applications and NAT. Many methodshas been developed and implemented to solve connectivity between peers behind NAT devices.Nevertheless, various NATing types can’t communicate with one another. In this work, we are going tostudy the impact of NAT types on the start-up delay time of peers in P2P streaming systems. We willdemonstrate the ability of NATing to expel peers in P2P live streaming systems. A new neighbour selectingalgorithm will be proposed. This algorithm will utilize NAT-types configurations as a parameter. We haveutilized NS2 simulator to show the performance of the new algorithm in increasing the connectivity,reducing the number of expelled peers and implementing of locality.

  8. On the Design and Implementation of Structured P2P VPNs

    Wolinsky, David Isaac; Lee, Kyungyong; Liu, Yonggang; Xu, Jiangyan; Boykin, P Oscar; Figueiredo, Renato


    Centralized Virtual Private Networks (VPNs) when used in distributed systems have performance constraints as all traffic must traverse through a central server. In recent years, there has been a paradigm shift towards the use of P2P in VPNs to alleviate pressure placed upon the central server by allowing participants to communicate directly with each other, relegating the server to handling session management and supporting NAT traversal using relays when necessary. Another, less common, approach uses unstructured P2P systems to remove all centralization from the VPN. These approaches currently lack the depth in security options provided by other VPN solutions, and their scalability constraints have not been well studied. In this paper, we propose and implement a novel VPN architecture, which uses a structured P2P system for peer discovery, session management, NAT traversal, and autonomic relay selection and a central server as a partially-automated public key infrastructure (PKI) via a user-friendly web inte...

  9. P2P Lending Risk Contagion Analysis Based on a Complex Network Model

    Qi Wei


    Full Text Available This paper analyzes two major channels of P2P lending risk contagion in China—direct risk contagion between platforms and indirect risk contagion with other financial organizations as the contagion medium. Based on this analysis, the current study constructs a complex network model of P2P lending risk contagion in China and performs dynamics analogue simulations in order to analyze general characteristics of direct risk contagion among China’s online P2P lending platforms. The assumed conditions are that other financial organizations act as the contagion medium, with variations in the risk contagion characteristics set under the condition of significant information asymmetry in Internet lending. It is indicated that, compared to direct risk contagion among platforms, both financial organizations acting as the contagion medium and information asymmetry magnify the effect of risk contagion. It is also found that the superposition of media effects and information asymmetry is more likely to magnify the risk contagion effect.

  10. Relay Ability Estimation and Topology Control Using Multidimensional Context Parameters for Mobile P2P Multicast

    Kubo Hiroyuki


    Full Text Available We focus on mobile P2P multicast, in which mobile end nodes not only act as receivers but also relay the received stream forward to others. In mobile P2P multicast, negative effects caused by the change of available bandwidth and the disconnection of mobile nodes are propagated to the downstream nodes. To solve this problem, we developed a novel node-allocation framework using the multidimensional context parameters of each mobile node, which include available bandwidth, disconnection rate, and the remaining battery capacity. Considering the significance of each parameter, our method integrates these parameters into a single parameter called relay ability. Taking the relay ability into account, each node is allocated to the multicast topology to minimize the negative effects mentioned above. To test our method, we applied our framework to conventional P2P multicast topology and show the results from comparative evaluations through computer simulation.

  11. Distributed Algorithms for Improving Search Efficiency in P2P Overlays

    Chittaranjan Hota


    Full Text Available Peer-to-peer (P2P overlay is a distributed application architecture in which peers share their resources. Peers are equally privileged, equipotent participants in the application. Several algorithms for enhancing P2P file searching have been proposed in the literature. In this paper, we have proposed a unique approach of reducing the P2P search complexity and improving search efficiency by using distributed algorithms. In our approach a peer mounts other popular peer’s files and also replicates other popular files or critical files identified using a threshold value. Once a file is mounted, file access requests can be serviced by transparently retrieving the file and sending it to the requesting peer. Replication used in this work improves the file retrieval time by allowing parallel transfer. We have shown the performance analysis of our proposed approach which shows improvement in the search efficiency.

  12. Ginkgolide B Inhibits Human Bladder Cancer Cell Migration and Invasion Through MicroRNA-223-3p

    Yi Zhi


    Full Text Available Background/Aims: Ginkgolide B (GB is currently used as an anticancer drug for treatment of some malignant cancers. However, whether it may have therapeutic effects on bladder cancer remains unknown. Here, we studied the effects of GB on bladder cancer cells. Methods: Bladder cells were treated with different doses of GB, and the effects on ZEB1 and microRNA-223-3p (miR-223-3p were analyzed by RT-qPCR and/or Western blot. Prediction of a regulatory relationship between miR-93 and 3'-UTR of Beclin-1 mRNA was performed by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Results: We found that GB dose-dependently decreased ZEB1 protein, but not mRNA, in bladder cancer cells, resulting in suppression of cell invasion. Moreover, in bladder cancer cells, GB dose-dependently decreased the levels of miR-223-3p, which suppressed the protein translation of ZEB1 through binding to 3'-UTR of ZEB1 mRNA. Overexpression of miR-223-3p decreased ZEB1 protein, while depletion of miR-223-3p increased ZEB1 protein in bladder cancer cells. Conclusion: GB inhibits bladder cancer cell invasiveness through suppressing ZEB1 protein translation via upregulating miR-223-3p.

  13. MiR-525-3p enhances the migration and invasion of liver cancer cells by downregulating ZNF395.

    Fei Pang

    Full Text Available Liver cancer is one of leading causes of cancer-related deaths. A deeper mechanistic understanding of liver cancer could lead to the development of more effective therapeutic strategies. In our previous work, we screened 646 miRNAs and identified 11 that regulate liver cancer cell migration. The current study shows that miR-525-3p is frequently up-regulated in liver cancer tissues, and enhanced expression of miR-525-3p can promote liver cancer cell migration and invasion. Zinc finger protein 395 (ZNF395 is the direct functional target gene for miR-525-3p, and it is frequently down-regulated in liver cancer tissues. High expression of ZNF395 can significantly inhibit while knockdown of ZNF395 expression can markedly enhance the migration and invasion of liver cancer cells, suggesting that ZNF395 suppresses metastasis in liver cancer. Down-regulation of ZNF395 can mediate miR-525-3p induced liver cancer cell migration and invasion. In conclusion, miR-525-3p promotes liver cancer cell migration and invasion by directly targeting ZNF395, and the fact that miR-525-3p and ZNF395 both play important roles in liver cancer progression makes them potential therapeutic targets.

  14. Structure and Function of the PLAA/Ufd3-p97/Cdc48 Complex

    Qiu, Liyan; Pashkova, Natasha; Walker, John R.; Winistorfer, Stanley; Allali-Hassani, Abdellah; Akutsu, Masato; Piper, Robert; Dhe-Paganon, Sirano (Iowa); (Toronto)


    PLAA (ortholog of yeast Doa1/Ufd3, also know as human PLAP or phospholipase A2-activating protein) has been implicated in a variety of disparate biological processes that involve the ubiquitin system. It is linked to the maintenance of ubiquitin levels, but the mechanism by which it accomplishes this is unclear. The C-terminal PUL (PLAP, Ufd3p, and Lub1p) domain of PLAA binds p97, an AAA ATPase, which among other functions helps transfer ubiquitinated proteins to the proteasome for degradation. In yeast, loss of Doa1 is suppressed by altering p97/Cdc48 function indicating that physical interaction between PLAA and p97 is functionally important. Although the overall regions of interaction between these proteins are known, the structural basis has been unavailable. We solved the high resolution crystal structure of the p97-PLAA complex showing that the PUL domain forms a 6-mer Armadillo-containing domain. Its N-terminal extension folds back onto the inner curvature forming a deep ridge that is positively charged with residues that are phylogenetically conserved. The C terminus of p97 binds in this ridge, where the side chain of p97-Tyr805, implicated in phosphorylation-dependent regulation, is buried. Expressed in doa1{Delta} null cells, point mutants of the yeast ortholog Doa1 that disrupt this interaction display slightly reduced ubiquitin levels, but unlike doa1{Delta} null cells, showed only some of the growth phenotypes. These data suggest that the p97-PLAA interaction is important for a subset of PLAA-dependent biological processes and provides a framework to better understand the role of these complex molecules in the ubiquitin system.

  15. Research of the lime rich portions of the CaO-SiO2-P2O5 system

    Puertas, F.


    Full Text Available A detailed study has been carried out to explain the influence of Pp^ in silicate phases and in industrial raw meals upon their mineralogical compositions. Some compositions in the lime rich portion of the CaO-SiO2-P2O5 system are studied using laboratory reactants. The results showed that the compositions lying in the range of CaO = 69,5-70,1; P2O5= 16,2-19,5; SiO2 = 11,1-13,6 (wt% give, at 1000 °C, the mineralogical composition (HAp, β-C2S, C5PS which can develop a high strength in chemically bonded ceramics. At high temperature (1400 ºC these compositions give a large amount of silicocarnotite (C5PS and 2C2.C3P phase. A selected composition of the industrial raw meal with added phosphate: CaO = 62,66; SiO2 -= 17,33; Al2O3 = 4,88; Fe2O3 = 4,29; P2O5 = 8,73; MgO = 2,09 (wt% forms at 1350 ºC a clinker containing high amount of α-C2S to replace C3S. Such a clinker may have good applications in the synthesis of active belite cement.Se estudia con detalle la influencia del P2O5 sobre las composiciones mineralógicas en fases silicato y en un crudo industrial. Asimismo se estudian algunas composiciones de la zona rica en cal del sistema CaO-SiO2-P2O5 utilizando reactivos químicos. Los resultados demuestran que, a presión atmosférica y recinto abierto, las composiciones dentro de los límites CaO = 69,5-70,1; P2O5= 16,2-19,5; SiO2 = 11,1-13,6 (% en peso dan a 1000ºC la composición mineralógica siguiente: HAp, β-C2S, C5PS; pueden desarrollar una alta resistencia química en "chemically bonded ceramics". A altas temperaturas (1400°C producen una elevada proporción de silicocarnotita (C5PS S y la fase 2C2.C3P. Con la adición de fosfato a una determinada dosificación de crudo industrial (CaO = 62,66; SiO2 = 17,33; Al2O3 = 4,88; Fe2O3 = 4,29; P2O5 = 8,73; MgO = 2,09 -% en peso- se forma, a 1350°C, un clinker que contiene una alta proporción de α-C2S que reemplaza al C3S. Este clinker puede tener buenas aplicaciones en la obtención de

  16. Enabling Co-located Learning over Mobile Ad Hoc P2P with LightPeers

    Bent Guldbjerg Christensen


    Full Text Available This paper presents LightPeers – a new mobile P2P framework specifically tailored for use in a nomadic learning environment. A set of key requirements for the framework is identified based on nomadic learning, and these requirements are used as outset for designing and implementing the architecture and protocols. The main contributions of the LightPeers framework are: a mobile P2P framework including a specialized robust messaging protocol resilient to changes in the network topology, an developers API, and a suite of LightPeers applications supporting nomadic learning prototyping key features of LightPeers.

  17. Quantum electrodynamics corrections to the 2P fine splitting in Li.

    Puchalski, Mariusz; Pachucki, Krzysztof


    We consider quantum electrodynamics (QED) corrections to the fine splitting E(2P_{3/2})-E(2P_{1/2}) in the Li atom. We derive complete formulas for the mα^{6} and mα^{7}lnα contributions and calculate them numerically using highly optimized, explicitly correlated basis functions. The obtained results are in agreement with the most recent measurement, helping to resolve discrepancies between former ones and lay the foundation for the investigation of QED effects in light, many-electron atoms.

  18. Cluster based hierarchical resource searching model in P2P network

    Yang Ruijuan; Liu Jian; Tian Jingwen


    For the problem of large network load generated by the Gnutella resource-searching model in Peer to Peer (P2P) network, a improved model to decrease the network expense is proposed, which establishes a duster in P2P network,auto-organizes logical layers, and applies a hybrid mechanism of directional searching and flooding. The performance analysis and simulation results show that the proposed hierarchical searching model has availably reduced the generated message load and that its searching-response time performance is as fairly good as that of the Gnutella model.

  19. A Participation-Based Trust Model for Mobile P2P Networks

    Kai Liu


    Full Text Available Mobile peer-to-peer (P2P networks have become increasingly popular, but the attacks and selfness of peers make it vulnerable. In this paper, a trust model based on participation is proposed for mobile P2P networks (called ParticipationTrust. It could deal with the egoistic and malicious behaviors. And the level of peer’s participation could be calculated through the transaction participation and recommendation participation. A set of experiments show that the model is rational and effective

  20. Enabling Co-located Learning over Mobile Ad Hoc P2P with LightPeers

    Christensen, Bent Guldbjerg; Kristensen, Mads Darø; Hansen, Frank Allan


    This paper presents LightPeers – a new mobile P2P framework specifically tailored for use in a nomadic learning environment. A set of key requirements for the framework is identified based on nomadic learning, and these requirements are used as outset for designing and implementing the architecture...... and protocols. The main contributions of the LightPeers framework are: a mobile P2P framework including a specialized robust messaging protocol resilient to changes in the network topology, an developers API, and a suite of LightPeers applications supporting nomadic learning prototyping key features of LightPeers....

  1. Efficient content-based P2P image retrieval using peer content descriptions

    Muller, Wolfgang T.; Eisenhardt, Martin; Henrich, Andreas


    Peer-to-peer (P2P) networks are overlay networks that connect independent computers (also called nodes or peers). In contrast to client/server solutions, all nodes offer and request services from other peers in a P2P network. P2P networks are very attractive in that they harness the computing power of many common desktop machines and necessitate little administrative overhead. While the resulting computing power is impressive, efficiently looking up data still is the major challenge in P2P networks. Current work comprises fast lookup of one-dimensional values (Distributed Hash Tables, DHT) and retrieval of texts using few keywords. However, the lookup of multimedia data in P2P networks is still attacked by very few groups. In this paper, we present experiments with efficient Content Based Image Retrieval in a P2P environment, thus a P2P-CBIR system. The challenge in such systems is to limit the number of messages sent, and to maximize the usefulness of each peer contacted in the query process. We achieve this by distributing peer data summaries over the network. Obviously, the data summaries have to be compact in order to limit the communication overhead. We propose an CBIR scheme based on a compact peer data summary. This peer data summary relies on cluster frequencies. To obtain the compact representation of a peer's collection, a global clustering of the data is efficiently calculated in a distributed manner. After that, each peer publishes how many of its images fall into each cluster. These cluster frequencies are then used by the querying peer to contact only those peers that have the largest number of images present in one cluster given by the query. In our paper we further detail the various challenges that have to be met by the designers of such a P2P-CBIR, and we present experiments with varying degree of data replication (duplicates of images), as well as quality of clustering within the network.

  2. Si/SiO2/p-Si结构的电学特性

    马自军; 马书懿



  3. A Hybrid P2P Approach to Service Discovery in the Cloud

    Nor Aniza Abdullah


    Full Text Available Highly scalable techniques for service discovery are key to the efficient use of Cloud resources, since the Cloud computing appears to be part of the mainstream computing in a few years. We embarked on a preliminary study on Cloud service discovery by adopting an unstructured P2P paradigm. We developed an efficient mechanism for routing of service requests by coupling a number of components: one-hop replication, semanticaware message routing, topology reorganization, and supernodes. A number of experiments were carried out that demonstrated the expected performance of the proposed P2P search scheme.

  4. Photoionization of Ge13+ in the Inner-Shell 2p Excitation Energy Region

    LIANG Gui-Yun; ZHAO Gang


    The photoionization cross sections of the levels belonging to the ground configuration [Ne]3s23p63d of Ge13+ are investigated using the fully relativistic R-matrix method in the 2p-3d excitation region.The photoionization cross section is dominated by 2p-3d resonances.The detailed resonance structures are described and analysed with the resonance positions,widths and oscillator strengths to be determined.Good agreement is obtained between the length and velocity forms of the resonallce oscillator strengths.The relative difference is less than 8%.

  5. 2S-2P Lamb shift and 2S hyperfine splitting in muonic hydrogen

    Antognini, Aldo; Biraben, François; Indelicato, Paul; Nez, François; Pohl, Randolf


    The 7 standard deviations between the proton rms charge radius from muonic hydrogen and the CODATA-10 value from hydrogen spectroscopy and electron-scattering has caused considerable discussions. Here, we review the theory of the 2S-2P Lamb shift and 2S hyperfine splitting in muonic hydrogen combining the published contributions and theoretical approaches. The prediction of these quantities is necessary for the determination of both proton charge and Zemach radii from the two 2S-2P transition frequencies measured in muonic hydrogen.

  6. Integrating XQuery and P2P in MonetDB/XQuery*

    Zhang, Ying; Boncz, Peter; Arenas, M; Hidders, J.


    MonetDB/XQuery* is a fully functional publicly available XML DBMS that has been extended with distributed and P2P data management functionality. Our (minimal) XQuery language extension XRPC adds the concept of RPC to XQuery, and exploits the set-at-a-time database processing model to optimize the networking cost through a technique called Bulk RPC. We describe our approach to include the services offered by diverse P2P network structures (such as DHTs), in a way that avoids any further intrus...

  7. Cross-layer P2P traffic engineering in content-based networks.

    Zhang, Xu


    Peer-to-peer (P2P) content sharing applications account for a significant fraction of the traffic volumes and is expected to increase [1]. Data is distributed to a large population of end client peers from end source peers in P2P systems, without the need for big investments to deploy servers. The costs of the content distribution are thus shared among end users and Internet service providers (ISPs). Consequently, negative impacts, such as increased inter-ISP traffic in particular, have becom...

  8. Chemical preparation, kinetics of thermal behavior and infrared studies of Pb3(P3O92.3H2O and Cd3(P3O92.14H2O

    Said Belaaouad


    Full Text Available Chemical preparation, thermal behavior, kinetic and IR studies are given for the cyclotriphosphates Pb3(P3O92.3H2O and Cd3(P3O92.14H2O. The later cyclotriphosphates have never been studied except their crystallographic characterization and are stable in the conditions of temperature and pressure of our laboratory until 343K. The final products of the dehydration and calcination of Pb3(P3O92.3H2O and Cd3(P3O92.14H2O, under atmospheric pressure, are respectively their long chain polyphosphates, [Pb(PO32]∞ and β[Cd(PO32]∞. The intermediate product of the dehydration of Cd3(P3O92.14H2O, under atmospheric pressure, is its long chain polyphosphate form α, α[Cd(PO32]. [Pb(PO32]∞ and β[Cd(PO32]∞ are stable until their melting points at respectively 946K and 1153K. Two different methods, Ozawa and KAS have been selected in order to study the kinetics of thermal behavior of the cyclotriphosphates Pb3(P3O92.3H2O and Cd3(P3O92.14H2O for the first time. The kinetic and thermodynamic features of the dehydration, of the cited cyclotriphosphates, were determined and discussed on the basis of their crystalline structure. [Pb(PO32]∞, α[Cd(PO32] and β[Cd(PO32] have many applications in industry such as corrosion inhibitors.

  9. Relationship between miRNA-338-3p expression and progression and prognosis of human colorectal carcinoma

    Sun Kai; Su Guiyuan; Deng Haijun; Dong Jingqing; Lei Shangtong; Li Guoxin


    Background miR-338-3p is a recently discovered miRNA and is involved in cell differentiation.However,few data are yet available on the aberrant expression of miR-338-3p in human colorectal carcinoma (CRC).This work aimed to investigate the relationship between miR-338-3p expression pattern and clinicopathological features of human CRC and the possible regulative mechanisms.Methods The 40 CRC,adjacent nontumorous tissues and 2 human CRC-derived cell lines (SW-480 and SW-620) were collected,respectively,and the total RNA and protein were isolated routinely.The miR-338-3p expression pattern was detected by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Northern blotting.Smoothened (SMO,possible target of miR-338-3p) mRNA and corresponding protein expression pattern were detected by semiquantitative RT-PCR and Western blotting.miR-338-3p expression patterns were compared between nontumor mucosa and CRC samples,graded by progression-related factors.Disease outcome was calculated by Kaplan-Meier survival analysis to determine whether miR-338-3p was related to disease-free survival (DFS) and overall survival (OS) of patients.Moreover,SMO 3'-UTR fragment was PCR amplified from genome DNA of human colon and inserted into a luciferase reporter plasmid.The luciferase reporter plasmid construct was then transfected into CRC cells together with pre-miR-338-3p or anti-miR-338-3p and the luciferase activity in the transfected cells was detected.Results The expression of miR-338-3p was significantly downregulated in CRCs than those in the adjacent nontumorous tissues,and the value was negatively related to advanced TNM stage and local invasion (P <0.01).Furthermore,miR338-3p value was decreased markedly in SW-620 cell line relative to SW-480 (P <0.01).Low expression of miR-338-3p was associated with unfavorable outcome in DFS but not in OS independent of clinical covariates.Moreover,RT-PCR and Western blotting analysis demonstrated that there was no

  10. miR-4782-3p Inhibited Non-Small Cell Lung Cancer growth via USP14

    Ning Wu


    Full Text Available Background: Lung cancer is the leading cause of cancer-related mortality worldwide, with near 1.4 million deaths each year. NSCLC accounts for nearly 85% of all case of lung cancer. MiRNAs play important roles in regulation of gene expression at the post-transcriptional level. MiRNAs profiles may predict prognosis and disease recurrence in early-stage NSCLC. Our previous study proved that over-expression of ubiquitin specific peptidase 14 (USP14, a deubiquitinating enzyme, was associated with favorable prognosis in NSCLC patients and promoted tumor cells proliferation. Here, we tried to identify which miRNAs targeted USP14, and the roles of these miRNAs in NSCLC. Methods: MiR-4782-3p and its potential targeted genes were identified by bioinformatics algorithm. Dual luciferase reporter assay system was used to analyze the interaction between miR-4782-3p and targeted genes. Cell proliferation was assayed by MTT and BdU assay. MiRNAs and mRNA expression were assayed by qRT-PCR. USP14 protein level was assayed by Western blot. The role of miR-4782-3p in patients survival was revealed by Kaplan-Meier plot of overall survival. Results: Up-expression of miR-4782-3p in NSCLC cells decreased the USP14 expression. Down-expression of miR-4782-3p increased USP14 expression. In NSCLC specimen, Negative correlation between USP14 mRNA level and miR-4782-3p level was identified. Higher miR-4782-3p expression is associated with longer survival. USP14, ZEB2, XIAP overexpression reversed the inhibitory effect of miR-4782-3p. Conclusions: High expression of miR-4782-3p was associated with favorable prognosis in NSCLC patients. MiR-4782-3p inhibited cell proliferation in NSCLC by targeting USP14, ZEB2 and XIAP.

  11. Reactions of N+ (3P) ions with H2 and HD molecules at low temperatures

    Grozdanov, Tasko P.; McCarroll, Ronald; Roueff, Evelyne


    formation of ND+. The calculated value is consistent with the available experimental data. Conclusions: The present results allow for the determination of reaction rate coefficients for any given distribution of specific fine structure and rotational state populations of the reactants. In interstellar conditions, where N+ is in its 3P0 state and para- and ortho-H2 respectively in J = 0 and J = 1. Our results enable a study of the influence of the ortho/para evolution of molecular hydrogen on the formation of nitrogen compounds.

  12. Electron-impact excitation of the (2p2) 1D and (2s2p) 1Po autoionizing states of helium

    Sise, Omer; Dogan, Mevlut; Okur, Ibrahim; Crowe, Albert


    An experimental study of the excitation of the (2p2) 1D and (2s2p) 1Po autoionizing states of helium by 250-eV electron impact is presented. The ejected-electron angular distributions and energy spectra are measured in coincidence with the corresponding scattered electrons for a scattering angle of -13∘ and for a range of ejected-electron angles in both the forward and backward directions. Resonance profiles are analyzed in terms of the Shore-Balashov parametrization to obtain the resonance asymmetry Aμ and yield Bμ parameters and the direct ionization cross section f. The spectra and their parameters are compared to the previous measurements of Lower and Weigold [J. Phys. B.JPAPEH0953-407510.1088/0953-4075/23/16/023 23, 2819 (1990)] and McDonald and Crowe [J. Phys. BJPAPEH0953-407510.1088/0953-4075/26/17/021 26, 2887 (1993)]. Comparison is also made with the recent theoretical triply differential cross-section calculations based on the first and second Born approximations. In general, good qualitative agreement is found between the experimental results. Some differences are found at the forward and backward directions. These differences in the shape and magnitude of the cross sections are attributed to the different incoming electron energies used in the experiments. The second Born approximation with inclusion of the three-body Coulomb interaction in the final state agrees reasonably well with experiments in the binary region. However, the 1Po resonance yield parameter Bμ is significantly overestimated at the recoil region, giving a relatively large recoil peak, in contradiction to the experiment. There is also a discrepancy between the two theories available for the 1D resonance yield parameter Bμ in this region. Remaining discrepancies between theories and experiments are also discussed.

  13. Database selection and result merging in P2P web search

    Chernov, S.; Serdyukov, P.; Bender, M.; Michel, S.; Weikum, G.; Zimmer, C.


    Intelligent Web search engines are extremely popular now. Currently, only the commercial centralized search engines like Google can process terabytes of Web data. Alternative search engines fulfilling collaborative Web search on a voluntary basis are usually based on a blooming Peer-to-Peer (P2P) te

  14. Scaling laws for file dissemination in P2P networks with random contacts

    Nunez-Queija, R.; Prabhu, B.


    In this paper we obtain the scaling law for the mean broadcast time of a file in a P2P network with an initial population of N nodes. In the model, at Poisson rate λ a node initiates a contact with another node chosen uniformly at random. This contact is said to be successful if the contacted node p

  15. Scaling laws for file dissemination in P2P networks with random contacts

    Núñez-Queija, R.; Prabhu, B.


    In this paper we obtain the scaling law for the mean broadcast time of a file in a P2P network with an initial population of N nodes. In the model, at Poisson rate lambda a node initiates a contact with another node chosen uniformly at random. This contact is said to be successful if the contacted n

  16. IPTV-RM: A Resources Monitoring Architecture for P2P IPTV Systems

    Wenxian Wang


    Full Text Available Resources monitoring is an important problem of the overall efficient usage and control of P2P IPTV systems. The resources of IPTV can include all distributing servers, programs and peers. Several researches have tried to address this issue, but most of them illuminated P2P traffic characterization, identification and user behavior. The main contributions of this paper are twofold. Firstly, a resources monitoring architecture for P2P IPTV systems, IPTV-RM, was presented based on previous work. The monitoring architecture employs a hierarchical structure and provides systemic monitoring including resources discovery, relative information extraction and analysis, trace and location. It gives a systematic framework for IPTV resources monitoring. Secondly, a distributed program crawling system (DMP-Crawler was first proposed to collect information of programs, and a peer crawling system was put forward to harvest peers of a program. The results show that they are efficient and can be used for resource collection of other P2P system. 

  17. From P2P to Web services and grids peers in a client/server world

    Taylor, Ian J


    Provides an overview of peer-to-peer (P2P) technologies that have revolutionized the way we think about distributed computing and the internet. This book compares these technologies to alternative solutions, most notably web services and Grid computing but also other technologies, such as client/server based systems and agent technologies.

  18. Identifying file-sharing P2P traffic based on traffic characteristics


    This article focuses on identifying file-sharing peer-to-peer (P2P) (such as BitTorrent (BT)) traffic at the borders of a stub network. By analyzing protocols and traffic of applications, it is found that file-sharing P2P traffic of a single user differs greatly from traditional and other P2P (such as QQ) applications' traffic in the distribution of involved remote hosts and remote ports. Therefore, a method based on discreteness of remote hosts (RHD) and discreteness of remote ports (RPD) is proposed to identify BT-like traffic. This method only relies on flow information of each user host in a stub network, and no packet payload needs to be monitored. At intervals, instant RHD for concurrent transmission control protocol and user datagram protocol flows for each host are calculated respectively through grouping flows by the stub network that the remote host of each flow belongs to. On given conditions, instant RPD are calculated through grouping flows by the remote port to amend instant RHD. Whether a host has been using a BT-like application or not can be deduced from instant RHD or average RHD for a period of time. The proposed method based on traffic characteristics is more suitable for identifying protean file-sharing P2P traffic than content-based methods. Experimental results show that this method is effective with high accuracy.

  19. Electronic Structure and Optical Properties Of EuIn2P2

    Singh, Nirpendra


    The electronic structures and, optical and magneto‐optical properties of a newly found Zintl compound EuIn2P2 have been investigated within the density‐functional theory using the highly precise full‐potential linear‐augmented‐plane‐wave method. Results of detailed investigation of the electronic structure and related properties are reported.

  20. Thermodynamics around the first-order ferromagnetic phase transition of Fe2P single crystals

    Hudl, M.; Campanini, D.; Caron, L.; Höglin, V.; Sahlberg, M.; Nordblad, P.; Rydh, A.


    The specific heat and thermodynamics of Fe2P single crystals around the first-order paramagnetic to ferromagnetic (FM) phase transition at TC≃217K are empirically investigated. The magnitude and direction of the magnetic field relative to the crystal axes govern the derived H−T phase diagram. Striki

  1. Traceable P2P record exchange: a database-oriented approach

    Fengrong LI; Takuya IIDA; Yoshiharu ISHIKAWA


    In recent years,peer-to-peer (P2P) technologies are used for flexible and scalable information exchange in the Internet,but there exist problems to be solved for reliable information exchange.It is important to trace how data circulates between peers and how data modifications are performed during the circulation before reaching the destination for enhancing the reliability of exchanged information.However,such lineage tracing is not easy in current P2P networks,since data replications and modifications are performed independently by autonomous peers--this creates a lack of reliability among the records exchanged.In this paper,we propose a framework for traceable record exchange in a P2P network.By managing historical information in distributed peers,we make the modification and exchange histories of records traceable.One of the features of our work is that the database technologies are utilized for realizing the framework.Histories are maintained in a relational database in each peer,and tracing queries are written in the datalog query language and executed in a P2P network by cooperating peers.This paper describes the concept of the framework and overviews the approach to query processing.

  2. SemTrust: A Semantic Reputation System in P2P-Based Semantic Web

    WANG Wei; ZENG Guosun; YUAN Lulai


    A reputation mechanism is introduced in P2P-based Semantic Web to solve the problem of lacking trust. It enables Semantic Web to utilize reputation information based on semantic similarity of peers in the network. This approach is evaluated in a simulation of a content sharing system and the experiments show that the system with reputation mechanism outperforms the system without it.

  3. Is the X (3915 ) the χc 0(2 P )?

    Olsen, Stephen Lars


    The Particle Data Group Meson Summary Table lists the X (3915 ) meson, an ω J /ψ mass peak seen in B →K ω J /ψ decays and γ γ →ω J /ψ two-photon fusion reactions, as the χc 0(2 P ), the 23P0 charmonium state. Here, with some reasonable assumptions, it is shown that if the X (3915 ) is the χc 0(2 P ), the measured strength of the γ γ →X (3915 ) signal implies an upper limit on the branching fraction B (χc 0(2 P )→ω J /ψ ) that is below a lower limit inferred for the same quantity from the B →K X (3915 ) decay rate. Also, the absence any signal for X (3915 )→D0D¯0 in B+→K+D0D¯0 decays is used to infer the limit B (X (3915 )→D0D¯0)Zweig-Iizuka suppressed, should be relatively rare. These, plus reasons given earlier by Guo and Meissner, raise concerns about the X (3915 )=χc 0(2 P ) assignment.

  4. Visual Display of 5p-arm and 3p-arm miRNA Expression with a Mobile Application

    Chao-Yu Pan


    Full Text Available MicroRNAs (miRNAs play important roles in human cancers. In previous studies, we have demonstrated that both 5p-arm and 3p-arm of mature miRNAs could be expressed from the same precursor and we further interrogated the 5p-arm and 3p-arm miRNA expression with a comprehensive arm feature annotation list. To assist biologists to visualize the differential 5p-arm and 3p-arm miRNA expression patterns, we utilized a user-friendly mobile App to display. The Cancer Genome Atlas (TCGA miRNA-Seq expression information. We have collected over 4,500 miRNA-Seq datasets from 15 TCGA cancer types and further processed them with the 5p-arm and 3p-arm annotation analysis pipeline. In order to be displayed with the RNA-Seq Viewer App, annotated 5p-arm and 3p-arm miRNA expression information and miRNA gene loci information were converted into SQLite tables. In this distinct application, for any given miRNA gene, 5p-arm miRNA is illustrated on the top of chromosome ideogram and 3p-arm miRNA is illustrated on the bottom of chromosome ideogram. Users can then easily interrogate the differentially 5p-arm/3p-arm expressed miRNAs with their mobile devices. This study demonstrates the feasibility and utility of RNA-Seq Viewer App in addition to mRNA-Seq data visualization.

  5. Structural and physical properties in the system ZnO-B 2O 3-P 2O 5-R nO m

    Li, Shengchun; Chen, Pei; Li, Yaogang


    The glass forming region in the quaternary system ZnO-B 2O 3-P 2O 5-R nO m has been determined. Glass transition temperature ( Tg), glass density ( ρ), thermal expansion coefficient ( α) and weight loss percentage ( WL) of these glasses were measured. The structure of (90- x- y)ZnO- xB 2O 3- yP 2O 5-10R nO m glass was characterized by infrared spectra (IR), Raman spectra and X-ray diffraction (XRD). Results of IR and Raman indicated that B 2O 3 participated in the glass network as a glass modifier and the IR band around 1440 cm -1 was ascribed to the v as(B-O-B) vibrations. Glass intensity increased with increase in B 2O 3 content. The disappearance of v s(P-O-P) vibration around 760 cm -1 indicated that P-O-B linkage was formed. XRD results revealed that the major crystalline phase changes with substitution of different amounts of B 2O 3: Zn 2P 2O 7 for 45ZnO- xB 2O 3-35P 2O 5-10R nO m glass, where x=0-20, and BPO 4 for 45ZnO- xB 2O 3-35P 2O 5-10R nO m glass, where x=30-40.

  6. Formation and decay of ( sup 3 P sub J )O atoms in the laser flash photolysis of chlorine dioxide (OClO) at 308 nm

    Colussi, A.J. (California Institute of Technology, Pasadena (USA))


    The primary quantum yields of O({sup 3}P{sub J}) and Cl({sup 2}P{sub 3/2}) atoms in the laser flash photolysis of OClO(g) at 308 nm and 298 K and the kinetics of the subsequent oxygen atom decay have been investigated by time-resolved atomic resonance fluorescence measurements. The determined quantum yields are {phi}{sub o} = 1.02 {plus minus} 0.05 and {phi}{sub Cl} < 0.01. Second-order rate constants for the reaction between O atoms and OClO are pressure dependent with a finite intercept. The rate constant for the bimolecular channel O + OClO {yields} O{sub 2} + ClO (1) has a value of k{sub 1} = (1.6 {plus minus} 0.4) {times} 10{sup {minus}13} cm{sup 3} molecule{sup {minus}1} s{sup {minus}1}. Termolecular reaction rates for O + OClO + Ar {yields} ClO{sub 3} + Ar (2) can be fit with k{sub 20} = (1.4 {plus minus} 0.3) {times} 10{sup {minus}31} cm{sup 6} molecule{sup {minus}2}s{sup {minus}1} and k{sub 2{infinity}} = (3.1 {plus minus} 0.8) {times} 10{sup {minus}11} cm{sup 3} molecule{sup {minus}1} s{sup {minus}1}.

  7. Luteolin Inhibits Ischemia/Reperfusion-Induced Myocardial Injury in Rats via Downregulation of microRNA-208b-3p.

    Chen Bian

    Full Text Available Luteolin (LUT, a kind of flavonoid which is extracted from a variety of diets, has been reported to convey protective effects of various diseases. Recent researches have suggested that LUT can carry out cardioprotective effects during ischemia/reperfusion (I/R. However, there have no reports on whether LUT can exert protective effects against myocardial I/R injury through the actions of specific microRNAs (miRs. The purpose of this study was to determine which miRs and target genes LUT exerted such function through.Expression of various miRs in perfused rat hearts was detected using a gene chip. Target genes were predicted with TargetScan, MiRDB and MiRanda. Anoxia/reoxygenation was used to simulate I/R. Cells were transfected by miR-208b-3p mimic, inhibitor and small interfering RNA of Ets1 (avian erythroblastosis virus E26 (v ets oncogene homolog 1. MiR-208b-3p and Ets1 mRNA were quantified by real-time quantitative polymerase chain reaction. The percentage of apoptotic cells was detected by annexin V-fluorescein isothiocyanate/propidium iodide dyeing and flow cytometry. The protein expression levels of cleaved caspase-3, Bcl-2, Bax, and Ets1 were examined by western blot analysis. A luciferase reporter assay was used to verify the combination between miR-208b-3p and the 3'-untranslated region of Ets1.LUT pretreatment reduced miR-208b-3p expression in myocardial tissue, as compared to the I/R group. And LUT decreased miR-208b-3p expression and apoptosis caused by I/R. However, overexpression of miR-208b-3p further aggravated the changes caused by I/R and blocked all the effects of LUT. Knockdown of miR-208b-3p expression also attenuated apoptosis, while knockdown of Ets1 promoted apoptosis. Further, the luciferase reporter assay showed that miR-208b-3p could inhibit Ets1 expression.LUT pretreatment conveys anti-apoptotic effects after myocardial I/R injury by decreasing miR-208b-3p and increasing Ets1 expression levels.

  8. Development of a chromosomally integrated metabolite-inducible Leu3p-alpha-IPM "off-on" gene switch.

    Maria Poulou

    Full Text Available BACKGROUND: Present technology uses mostly chimeric proteins as regulators and hormones or antibiotics as signals to induce spatial and temporal gene expression. METHODOLOGY/PRINCIPAL FINDINGS: Here, we show that a chromosomally integrated yeast 'Leu3p-alpha-IotaRhoMu' system constitutes a ligand-inducible regulatory "off-on" genetic switch with an extensively dynamic action area. We find that Leu3p acts as an active transcriptional repressor in the absence and as an activator in the presence of alpha-isopropylmalate (alpha-IotaRhoMu in primary fibroblasts isolated from double transgenic mouse embryos bearing ubiquitously expressing Leu3p and a Leu3p regulated GFP reporter. In the absence of the branched amino acid biosynthetic pathway in animals, metabolically stable alpha-IPM presents an EC(50 equal to 0.8837 mM and fast "OFF-ON" kinetics (t(50ON = 43 min, t(50OFF = 2.18 h, it enters the cells via passive diffusion, while it is non-toxic to mammalian cells and to fertilized mouse eggs cultured ex vivo. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that the 'Leu3p-alpha-IotaRhoMu' constitutes a simpler and safer system for inducible gene expression in biomedical applications.

  9. Prm3p is a pheromone-induced peripheral nuclear envelope protein required for yeast nuclear fusion.

    Shen, Shu; Tobery, Cynthia E; Rose, Mark D


    Nuclear membrane fusion is the last step in the mating pathway of the yeast Saccharomyces cerevisiae. We adapted a bioinformatics approach to identify putative pheromone-induced membrane proteins potentially required for nuclear membrane fusion. One protein, Prm3p, was found to be required for nuclear membrane fusion; disruption of PRM3 caused a strong bilateral defect, in which nuclear congression was completed but fusion did not occur. Prm3p was localized to the nuclear envelope in pheromone-responding cells, with significant colocalization with the spindle pole body in zygotes. A previous report, using a truncated protein, claimed that Prm3p is localized to the inner nuclear envelope. Based on biochemistry, immunoelectron microscopy and live cell microscopy, we find that functional Prm3p is a peripheral membrane protein exposed on the cytoplasmic face of the outer nuclear envelope. In support of this, mutations in a putative nuclear localization sequence had no effect on full-length protein function or localization. In contrast, point mutations and deletions in the highly conserved hydrophobic carboxy-terminal domain disrupted both protein function and localization. Genetic analysis, colocalization, and biochemical experiments indicate that Prm3p interacts directly with Kar5p, suggesting that nuclear membrane fusion is mediated by a protein complex.

  10. Propofol Inhibits Neurogenesis of Rat Neural Stem Cells by Upregulating MicroRNA-141-3p.

    Jiang, Qiliang; Wang, Yingwei; Shi, Xueyin


    Prolonged or high-dose exposure to anesthetics, such as propofol, can cause brain cell degeneration and subsequent long-term learning or memory deficits, particularly in the developing brain. However, the cellular and molecular mechanisms underlying the deleterious effects of propofol at certain stages of development remain unclear. In this study we found that propofol inhibited the proliferation, neuronal differentiation, and migration of neural stem cells (NSCs) while upregulating miR-141-3p. Silencing of miR-141-3p abrogated the effects of propofol on NSC neurogenesis. Propofol treatment downregulated IGF2BP2, a direct target of miR-141-3p, whereas overexpression of IGF2BP2 attenuated the effects of propofol and miR-141-3p on NSC neurogenesis. In short, propofol inhibits NSC neurogenesis through a mechanism involving the miR-141-3p/IGF2BP2 axis. Our results may provide a potential approach for preventing the neurodegenerative effects of propofol in the developing brain.

  11. Combination of MiR-103a-3p and Mesothelin Improves the Biomarker Performance of Malignant Mesothelioma Diagnosis

    Bryk, Oleksandr; Raiko, Irina; Pesch, Beate; Kollmeier, Jens; Bauer, Torsten T.; Brüning, Thomas


    Background For the detection of malignant mesothelioma no single biomarker with reasonable sensitivity and specificity has been described so far. Mesothelin, the most prominent blood-based biomarker, is characterized by high specificity but low sensitivity. It might be reasonable to combine biomarkers of different molecular classes in order to improve the overall performance. The aim of this study was to assess the performance of the combination of mesothelin and miR-103a-3p as blood-based biomarker for mesothelioma. Methods/Principal Findings Mesothelin concentration in plasma and miR-103a-3p levels in the cellular blood fraction were analyzed in 43 male mesothelioma patients and 52 male controls formerly exposed to asbestos. For the discrimination of epithelioid and biphasic mesothelioma from asbestos-exposed controls mesothelin and miR-103a-3p showed 74% and 89% sensitivity and 85% and 63% specificity, respectively. For the combination of mesothelin and miR-103a-3p a sensitivity of 95% and a specificity of 81% were calculated. Conclusions/Significance The results of this study show that the combination of mesothelin and miR-103a-3p improves the diagnostic performance of individual blood-based biomarker to detect malignant mesothelioma. The obtained results indicate that the use of biomarkers of different molecular classes might be a reasonable approach to assemble a biomarker panel. PMID:25469901

  12. Combination of MiR-103a-3p and mesothelin improves the biomarker performance of malignant mesothelioma diagnosis.

    Daniel G Weber

    Full Text Available For the detection of malignant mesothelioma no single biomarker with reasonable sensitivity and specificity has been described so far. Mesothelin, the most prominent blood-based biomarker, is characterized by high specificity but low sensitivity. It might be reasonable to combine biomarkers of different molecular classes in order to improve the overall performance. The aim of this study was to assess the performance of the combination of mesothelin and miR-103a-3p as blood-based biomarker for mesothelioma.Mesothelin concentration in plasma and miR-103a-3p levels in the cellular blood fraction were analyzed in 43 male mesothelioma patients and 52 male controls formerly exposed to asbestos. For the discrimination of epithelioid and biphasic mesothelioma from asbestos-exposed controls mesothelin and miR-103a-3p showed 74% and 89% sensitivity and 85% and 63% specificity, respectively. For the combination of mesothelin and miR-103a-3p a sensitivity of 95% and a specificity of 81% were calculated.The results of this study show that the combination of mesothelin and miR-103a-3p improves the diagnostic performance of individual blood-based biomarker to detect malignant mesothelioma. The obtained results indicate that the use of biomarkers of different molecular classes might be a reasonable approach to assemble a biomarker panel.

  13. miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110.

    Bijnsdorp, Irene V; Hodzic, Jasmina; Lagerweij, Tonny; Westerman, Bart; Krijgsman, Oscar; Broeke, Jurjen; Verweij, Frederik; Nilsson, R Jonas A; Rozendaal, Lawrence; van Beusechem, Victor W; van Moorselaar, Jeroen A; Wurdinger, Thomas; Geldof, Albert A


    The centrosome plays a key role in cancer invasion and metastasis. However, it is unclear how abnormal centrosome numbers are regulated when prostate cancer (PCa) cells become metastatic. CP110 was previously described for its contribution of centrosome amplification (CA) and early development of aggressive cell behaviour. However its regulation in metastatic cells remains unclear. Here we identified miR-129-3p as a novel metastatic microRNA. CP110 was identified as its target protein. In PCa cells that have metastatic capacity, CP110 expression was repressed by miR-129-3p. High miR-129-3p expression levels increased cell invasion, while increasing CP110 levels decreased cell invasion. Overexpression of CP110 in metastatic PCa cells resulted in a decrease in the number of metastasis. In tissues of PCa patients, low CP110 and high miR-129-3p expression levels correlated with metastasis, but not with the expression of genes related to EMT. Furthermore, overexpression of CP110 in metastatic PCa cells resulted in excessive-CA (E-CA), and a change in F-actin distribution which is in agreement with their reduced metastatic capacity. Our data demonstrate that miR-129-3p functions as a CA gatekeeper in metastatic PCa cells by maintaining pro-metastatic centrosome amplification (CA) and preventing anti-metastatic E-CA.

  14. Widely available active sites on Ni2P for electrochemical hydrogen evolution - insights from first principles calculations

    Hansen, Martin Hangaard; Stern, Lucas-Alexandre; Feng, Ligang;


    We present insights into the mechanism and the active site for hydrogen evolution on nickel phosphide (Ni2P). Ni2P was recently discovered to be a very active non-precious hydrogen evolution catalyst. Current literature attributes the activity of Ni2P to a particular site on the (0001) facet. In ...

  15. Regulatory role of the sequences downstream from nodD3 P1 promoter of Rhizobium meliloti


    The 660 bp region between nodD3 P1 promoter and the following coding region of Rhizobium meliloti has been studied.This region is designated "downstream sequences".It consists of two potential open reading frames,ORF1 and ORF2.Studies on the role of the downstream sequences on the activity of nodD3 P1 with nod D3(P1)-lacZ fusion show that deletion of the sequences containing ORF2 causes the increase of the activity of the fusion; on the contrary,addition of extra copies of ORF2 markedly decreases the activity of the fusion.These results indicate that the product of ORF2 plays a negative role in the expression of nod D3.

  16. Biomimetic-Inspired Infrared Sensors from Zn3P2 Microwires: Study of Their Photoconductivity and Infrared Spectrum Properties

    M. Israelowitz


    Full Text Available The fire beetle, Melanophila acuminata (Coleoptera: Buprestidae, senses infrared radiation at wavelengths of 3 and 10–25 microns via specialized protein-containing sensilla. Although the protein denatures outside of a biological system, this detection mechanism has inspired our bottom-up approach to produce single zinc phosphide microwires via vapour transport for IR sensing. The Zn3P2 microwires were immobilized and electrical contact was made by dielectrophoresis. Photoconductivity measurements have been extended to the near IR range, spanning the Zn3P2 band gaps. Purity and integrity of the Zn3P2 microwires including infrared light scattering properties were confirmed by infrared transmission microscopy. This biomimetic microwire shows promise for infrared chip development.

  17. Energetics, transition states, and intrinsic reaction coordinates for reactions associated with O(3P) processing of hydrocarbon materials

    Yan, Tianying; Hase, William L.; Doubleday, Charles


    Electronic structure calculations based on multiconfiguration wave functions are used to investigate a set of archetypal reactions relevant to O(3P) processing of hydrocarbon molecules and surfaces. These include O(3P) reactions with methane and ethane to give OH plus methyl or ethyl radicals, O(3P)+ethane to give CH3O+CH3, and secondary reactions of the OH product radical with ethane and the ethyl radical. Geometry optimization is carried out with CASSCF/cc-pVTZ for all reactions, and with CASPT2/cc-pVTZ for O(3P)+methane/ethane. Single-point energy corrections are applied with CASPT2, CASPT3, and MRCI+Q with the cc-pVTZ and cc-pVQZ basis sets, and the energies extrapolated to the complete basis set limit (CBL). Where comparison of computed barriers and energies of reaction with experiment is possible, the agreement is good to excellent. The best agreement (within experimental error) is found for MRCI+Q/CBL applied to O(3P)+methane. For the other reactions, CASPT2/CBL and MRCI+Q/CBL predictions differ from experiment by 1-5 kcal/mol for 0 K enthalpies of reaction, and are within 1 kcal/mol of the best-estimate experimental range of 0 K barriers for O(3P)+ethane and OH+ethane. The accuracy of MRCI+Q/CBL is limited mainly by the quality of the active space. CASPT2/CBL barriers are consistently lower than MRCI+Q/CBL barriers with identical reference spaces.

  18. Na/K-ATPase signaling regulates collagen synthesis through microRNA-29b-3p in cardiac fibroblasts.

    Drummond, Christopher A; Hill, Michael C; Shi, Huilin; Fan, Xiaoming; Xie, Jeffrey X; Haller, Steven T; Kennedy, David J; Liu, Jiang; Garrett, Michael R; Xie, Zijian; Cooper, Christopher J; Shapiro, Joseph I; Tian, Jiang


    Chronic kidney disease (CKD) is accompanied by cardiac fibrosis, hypertrophy, and dysfunction, which are commonly referred to as uremic cardiomyopathy. Our previous studies found that Na/K-ATPase ligands or 5/6th partial nephrectomy (PNx) induces cardiac fibrosis in rats and mice. The current study used in vitro and in vivo models to explore novel roles for microRNA in this mechanism of cardiac fibrosis formation. To accomplish this, we performed microRNA profiling with RT-qPCR based arrays on cardiac tissue from rats subjected to marinobufagenin (MBG) infusion or PNx. The analysis showed that a series of fibrosis-related microRNAs were dysregulated. Among the dysregulated microRNAs, microRNA (miR)-29b-3p, which directly targets mRNA of collagen, was consistently reduced in both PNx and MBG-infused animals. In vitro experiments demonstrated that treatment of primary cultures of adult rat cardiac fibroblasts with Na/K-ATPase ligands induced significant increases in the fibrosis marker, collagen protein, and mRNA expression compared with controls, whereas miR-29b-3p expression decreased >50%. Transfection of miR-29b-3p mimics into cardiac fibroblasts inhibited cardiotonic steroids-induced collagen synthesis. Moreover, a specific Na/K-ATPase signaling antagonist, pNaKtide, prevented ouabain-induced increases in collagen synthesis and decreases in miR-29b-3p expression in these cells. In conclusion, these data are the first to indicate that signaling through Na/K-ATPase regulates miRNAs and specifically, miR-29b-3p expression both in vivo and in vitro. Additionally, these data indicate that miR-29b-3p expression plays an important role in the formation of cardiac fibrosis in CKD.

  19. Lifetime measurement of the metastable 2{sup 3}P{sub 0} state in He-like uranium

    Toleikis, S. [Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., MS 88R0192, Berkeley, CA 94720-8101 (United States) and Texas A and M University, College Station, TX (United States)]. E-mail:; Manil, B. [GANIL, Caen (France); Bednarz, G. [IFUJ, Cracow (Poland); Berdermann, E. [GSI, Darmstadt (Germany); Beyer, H.F. [GSI, Darmstadt (Germany); Bosch, F. [GSI, Darmstadt (Germany); Braeuning-Demian, A. [GSI, Darmstadt (Germany); Gumberidze, A. [GSI, Darmstadt (Germany); Indelicato, P. [Universite P. et M. Curie, Paris (France); Kozhuharov, C. [GSI, Darmstadt (Germany); Liesen, D. [GSI, Darmstadt (Germany); Marrus, R. [University of California, Berkeley, CA (United States); Mokler, P.H. [GSI, Darmstadt (Germany); Stachura, Z. [INP, Cracow (Poland); Stoehlker, T. [GSI, Darmstadt (Germany); Warczak, A. [IFUJ, Cracow (Poland)


    The lifetime of the 2{sup 3}P{sub 0} state in He-like uranium has been measured in a beam-foil time-of-flight experiment at the Gesellschaft fur Schwerionenforschung accelerator facility with the result {tau}(2{sup 3}P{sub 0})=58.2(9.5)ps. With the measured lifetime it is possible to derive a value of {delta}E{sub 2s}{sup Lamb}=76.3+/-20.6eV for the n=2 Lamb shift in uranium.

  20. A proof of the linearity conjecture for k-blocking sets in PG(n, p3), p prime

    Lavrauw, Michel; Van de Voorde, Geertrui


    In this paper, we show that a small minimal k-blocking set in PG(n, q3), q = p^h, h >= 1, p prime, p >=7, intersecting every (n-k)-space in 1 (mod q) points, is linear. As a corollary, this result shows that all small minimal k-blocking sets in PG(n, p^3), p prime, p >=7, are Fp-linear, proving the linearity conjecture (see [7]) in the case PG(n, p3), p prime, p >= 7.

  1. SOX9 regulates microRNA miR-202-5p/3p expression during mouse testis differentiation

    Wainwright, Elanor N; Jorgensen, Joan S; Kim, Youngha;


    . Expression of the primary transcript of miR-202-5p/3p remained low in XY gonads in a conditional Sox9-null mouse model, suggesting that pri-miR-202 transcription is downstream of SOX9, a transcription factor that is both necessary and sufficient for male sex determination. We identified the pri-miR-202...... findings indicate that expression of the conserved gonad microRNA, miR-202-5p/3p, is downstream of the testis-determining factor SOX9, suggesting an early role in testis development....

  2. Measurement of the Hyperfine Structure and Isotope Shifts of the 3s23p2 3P2 to 3s3p3 3Do3 Transition in Silicon

    Lee, Siu Au


    The hyperfine structure and isotope shifts of the 3s23p2 3P2 to 3s3p3 3Do3 transition in silicon have been measured. The transition at 221.7 nm was studied by laser induced fluorescence in an atomic Si beam. For 29Si, the hyperfine A constant for the 3s23p2 3P2 level was determined to be -160.1+-1.3 MHz (1 sigma error), and the A constant for the 3s3p3 3Do3 level is -532.9+-0.6 MHz. This is the first time that these constants were measured. The isotope shifts (relative to the abundant isotope 28Si) of the transition were determined to be 1753.3+-1.1 MHz for 29Si and 3359.9+-0.6 MHz for 30Si. This is an improvement by about two orders of magnitude over a previous measurement. From these results we are able to predict the hyperfine structure and isotope shift of the radioactive 31Si atom, which is of interest in building a scalable quantum computer.

  3. High lying energy positions of doubly (2pns) {sup 1,3}P{sup o} and (2pnd) {sup 1,3}P{sup o} excited states of the beryllium atom

    Sakho, I., E-mail: [UFR Sciences and Technologies, Department of physics, University of Ziguinchor, Ziguinchor (Senegal)


    The Screening Constant by Unit Nuclear Charge (SCUNC) method is used to study (2pns) {sup 1,3}P{sup o} and (2pnd) {sup 1,3}P{sup o} autoionizing states of the beryllium atom. Energy positions are reported up to n=20. In addition, resonance widths of the (2pns) {sup 1}P{sup o} states also presented. The current results compared very well to available theoretical and experimental literature values up to n=15. The accurate data presented in this work may be of interest for future experimental and theoretical studies in the photoabsorption spectrum of Be. - Highlights: > Accurate energy positions of (2pns) {sup 1,3}P{sup o} and (2pnd) {sup 1,3}P{sup o} (n=3-20) autoionizing states of Be atoms. > Currently results compared very well to theoretical and experimental literature values up to n=15. > Presently data may be of interest for future experimental and theoretical studies in the photoabsorption spectrum of Be.

  4. miR-199a-3p 靶基因预测及生物信息学分析%Prediction of miR-199a-3p targets gene and its bioinformatics analysis

    谢小娟; 潘晶晶; 魏力强; 陈葳


    Objective To provide theoretical guidance for further research on the role of miR-1 99a-3p in formation and development of bladder cancer.Methods Mature sequence of miR-1 99a-3p was analyzed;target genes and transcription factors of miRNA-1 99a-3p were predicted,and the target genes were analyzed for gene ontology (GO)enrichment and Kyoto Encyclopedia of Genes and Genome (KEGG)pathway.Then TF-miRNA-mRNA network diagram was constructed.Results Sequences of miR-1 99a-3p were highly conserved in various species.In GO analysis,the target genes of miR-1 99a-3p were enriched in many biological processes,such as regulation of cellular process,regulation of macromolecule metabolic process,and regulation of biological process (P <0.01 ).In KEGG pathway,the target genes were mainly located in bacterial invasion pathway of epithelial cells,ECM-receptor interaction pathway,PI3K-Akt signaling pathway,MAPK signaling pathway,small cell lung cancer pathway,and proteoglycans pathway in the cancer (P <0.05).According to the TF-miRNA-mRNA network diagram,the important genes that might be regulated by miR-1 99a-3p were MYC,SP1,mTOR,NFκB,and NFκB1.Conclusion miR-1 99a-3p may directly target mTOR and participate in the formation and development of bladder cancer through regulating PI3K-Akt-mTOR signaling pathway.%目的:为深入研究 miR-199a-3p 在膀胱癌形成和发展中的作用提供理论依据。方法分析 miR-199a-3p 序列,预测其靶基因和转录因子,并对靶基因进行 GO 富集和 KEGG Pathway 分析;构建 TF-miR-199a-3p-靶基因网络调控图。结果miR-199a-3p 序列在多物种间具有高度保守性;GO 分析发现 miR-199a-3p 的靶基因参与细胞调节、代谢调节、细胞大分子生物合成等生物过程(P <0.01);KEGG Pathway 分析发现 miR-199a-3p 的靶基因显著富集在上皮细胞的细菌入侵通路、ECM 受体的相互作用通路、PI3K-Akt 信号通路、MAPK 信号通路、小细胞肺癌通路、癌症中的蛋白

  5. End-to-End Key Exchange through Disjoint Paths in P2P Networks

    Daouda Ahmat


    Full Text Available Due to their inherent features, P2P networks have proven to be effective in the exchange of data between autonomous peers. Unfortunately, these networks are subject to various security threats that cannot be addressed readily since traditional security infrastructures, which are centralized, cannot be applied to them. Furthermore, communication reliability across the Internet is threatened by various attacks, including usurpation of identity, eavesdropping or traffic modification. Thus, in order to overcome these security issues and allow peers to securely exchange data, we propose a new key management scheme over P2P networks. Our approach introduces a new method that enables a secret key exchange through disjoint paths in the absence of a trusted central coordination point which would be required in traditional centralized security systems.

  6. High Quality of Service on Video Streaming in P2P Networks using FST-MDC

    Jaganathan, Suresh; 10.5121/ijma.2011.3203


    Video streaming applications have newly attracted a large number of participants in a distribution network. Traditional client-server based video streaming solutions sustain precious bandwidth provision rate on the server. Recently, several P2P streaming systems have been organized to provide on-demand and live video streaming services on the wireless network at reduced server cost. Peer-to-Peer (P2P) computing is a new pattern to construct disseminated network applications. Typical error control techniques are not very well matched and on the other hand error prone channels has increased greatly for video transmission e.g., over wireless networks and IP. These two facts united together provided the essential motivation for the development of a new set of techniques (error concealment) capable of dealing with transmission errors in video systems. In this paper, we propose an flexible multiple description coding method named as Flexible Spatial-Temporal (FST) which improves error resilience in the sense of fra...

  7. Erv2p: characterization of the redox behavior of a yeast sulfhydryl oxidase

    Wang, Wenzhong; Winther, Jakob R; Thorpe, Colin


    centers that facilitate the transfer of reducing equivalents from the dithiol substrates of these oxidases to the isoalloxazine ring where the reaction with molecular oxygen occurs. The present study examines yeast Erv2p and compares the redox behavior of this ER luminal protein with the augmenter...... and with unfolded proteins. Rapid reaction studies confirm that reduction of the flavin center of Erv2p is rate-limiting during turnover with molecular oxygen. This comparison of the redox properties between members of the ERV/ALR family of sulfhydryl oxidases provides insights into their likely roles in oxidative......The FAD prosthetic group of the ERV/ALR family of sulfhydryl oxidases is housed at the mouth of a 4-helix bundle and communicates with a pair of juxtaposed cysteine residues that form the proximal redox active disulfide. Most of these enzymes have one or more additional distal disulfide redox...

  8. Reducing Network Traffic in Unstructured P2P Systems Using Top-k Queries

    Akbarinia, Reza; Valduriez, Patrick; 10.1007/s10619-006-8313-5


    A major problem of unstructured P2P systems is their heavy network traffic. This is caused mainly by high numbers of query answers, many of which are irrelevant for users. One solution to this problem is to use Top-k queries whereby the user can specify a limited number (k) of the most relevant answers. In this paper, we present FD, a (Fully Distributed) framework for executing Top-k queries in unstructured P2P systems, with the objective of reducing network traffic. FD consists of a family of algorithms that are simple but effec-tive. FD is completely distributed, does not depend on the existence of certain peers, and addresses the volatility of peers during query execution. We vali-dated FD through implementation over a 64-node cluster and simulation using the BRITE topology generator and SimJava. Our performance evaluation shows that FD can achieve major performance gains in terms of communication and response time.

  9. A Potential Approach of Internet Worm Propagation Based on P2P

    YAO Yu; LUO Xingrui; GAO Fuxiang; YU Ge


    Various kinds of active worms have been plunging into the network flows, which make the Internet security problem more serious. Our research on a potential propagation approach of active worms, P2P-based propagation approach, is given in this paper. To measure the propagating performance of our approach, the SEI (Susceptible-Exposed- Infected) propagation model is presented. It proves that with the idea of pure P2P architecture, worms can be hidden in the early stage of propagation, and then infect most of the hosts in a shorter period. By comparing our SEI propagation model with the Simple Epidemic Model, we observe that the size of a worm is a significant parameter which can affect the propagating performance. When the size of the worm becomes large, our approach can still show an excellent propagating performance.

  10. Using of P2P Networks for Acceleration of RTE Tasks Solving

    Adrian Iftene


    Full Text Available In the last years the computational Grids have become an important research area in large-scale scientific and engineering research. Our approach is based on Peer-to-peer (P2P networks, which are recognized as one of most used architectures in order to achieve scalability in key components of Grid systems. The main scope in using of a computational Grid was to improve the computational speed of systems that solve complex problems from Natural Language processing field. We will see how can be implemented a computational Grid using the P2P model, and how can be used SMB protocol for file transfer. After that we will see how we can use this computational Grid, in order to improve the computational speed of a system used in RTE competition [1], a new complex challenge from Natural Language processing field.

  11. Calculation of Computational Complexity for Radix-2 (p) Fast Fourier Transform Algorithms for Medical Signals.

    Amirfattahi, Rassoul


    Owing to its simplicity radix-2 is a popular algorithm to implement fast fourier transform. Radix-2(p) algorithms have the same order of computational complexity as higher radices algorithms, but still retain the simplicity of radix-2. By defining a new concept, twiddle factor template, in this paper, we propose a method for exact calculation of multiplicative complexity for radix-2(p) algorithms. The methodology is described for radix-2, radix-2 (2) and radix-2 (3) algorithms. Results show that radix-2 (2) and radix-2 (3) have significantly less computational complexity compared with radix-2. Another interesting result is that while the number of complex multiplications in radix-2 (3) algorithm is slightly more than radix-2 (2), the number of real multiplications for radix-2 (3) is less than radix-2 (2). This is because of the twiddle factors in the form of which need less number of real multiplications and are more frequent in radix-2 (3) algorithm.

  12. Brr2p RNA helicase with a split personality: insights into structure and function.

    Hahn, Daniela; Beggs, Jean D


    RNA helicases are involved in many cellular processes. Pre-mRNA splicing requires eight different DExD/H-box RNA helicases, which facilitate spliceosome assembly and remodelling of the intricate network of RNA rearrangements that are central to the splicing process. Brr2p, one of the spliceosomal RNA helicases, stands out through its unusual domain architecture. In the present review we highlight the advances made by recent structural and biochemical studies that have important implications for the mechanism and regulation of Brr2p activity. We also discuss the involvement of human Brr2 in retinitis pigmentosa, a degenerative eye disease, and how its functions in splicing might connect to the molecular pathology of the disease.

  13. A Fuzzy Reputation Management System with Punishment Mechanism for P2P Network

    Zhengbing Hu


    Full Text Available One way to minimize threats in P2P system is to exploit reputation to help evaluate the trustworthiness and predict the future behaviors of peers. In this paper, a set of parameters are identified for describing the trust of a peer. And a trust model based on fuzzy techniques is presented. The trust model uses Amplifier of Malicious Behavior (AMB to intensify malicious behaviors and helps peers identify the sly malicious peers. The recommendation credibility is evaluated by Recommendation Weight (RW and Recommendation Accuracy (RA. In simulation, this trust model has been shown to improve the efficiency of P2P system by significantly decreasing the number of inauthentic files on the network.

  14. WebVR——Web Virtual Reality Engine Based on P2P network

    zhihan LV


    Full Text Available WebVR, a multi-user online virtual reality engine, is introduced. The main contributions are mapping the geographical space and virtual space to the P2P overlay network space, and dividing the three spaces by quad-tree method. The geocoding is identified with Hash value, which is used to index the user list, terrain data, and the model object data. Sharing of data through improved Kademlia network model is designed and implemented. In this model, XOR algorithm is used to calculate the distance of the virtual space. The model greatly improves the hit rate of 3D geographic data search under P2P overlay network. Some data preprocessing methods have been adopted to accelerate the data transfer. 3D Global data is used for testing the engine. The test result indicates that, without considering the client bandwidth limit, the more users, the faster loading.

  15. DistHash: A robust P2P DHT-based system for replicated objects

    Dobre, Ciprian; Cristea, Valentin


    Over the Internet today, computing and communications environments are significantly more complex and chaotic than classical distributed systems, lacking any centralized organization or hierarchical control. There has been much interest in emerging Peer-to-Peer (P2P) network overlays because they provide a good substrate for creating large-scale data sharing, content distribution and application-level multicast applications. In this paper we present DistHash, a P2P overlay network designed to share large sets of replicated distributed objects in the context of large-scale highly dynamic infrastructures. We present original solutions to achieve optimal message routing in hop-count and throughput, provide an adequate consistency approach among replicas, as well as provide a fault-tolerant substrate.

  16. Prenatal diagnosis of a de novo inversion of chromosome (2)(p21q11).

    Hengstschläger, M; Mittermayer, C; Prusa, A R; Drahonsky, R; Repa, C; Deutinger, J; Bernaschek, G


    Prenatal diagnosis of "apparently balanced" chromosomal rearrangements, if not inherited from a parent, are problematic for genetic counsellors and families. Although the parents need to be informed about the increased risk of multiple congenital anomalies, the anomalies that the fetus is at risk can not be discussed unless a similar breakpoint and accompanying phenotype have been reported in the literature. In the reported case prenatal ultrasound examination revealed a massive hydrocephalus internus and IUGR. The karyotype of the fetus was inv(2)(p21q11) de novo. Postmortem examination revealed short palpebral fissures, hypertelorism, atypical nasiolabial configuration, microgenia, extended position of the fingers, atypical proximal inserted first toe, hydrocephalus internus, hypoplasia of the cerebellum and bulbi olfactorii, bilateral hypoplastic lungs, atrial septal defect II, small right ventricle, dysplasia of the pulmonary valve, hypoplastic pulmonary artery, right proximal ureterostenosis, hypoplastic gall bladder. This is the first description of a de novo inversion (2)(p21q11) in a fetus with multiple malformations.

  17. IP2P K-means: an efficient method for data clustering on sensor networks

    Peyman Mirhadi


    Full Text Available Many wireless sensor network applications require data gathering as the most important parts of their operations. There are increasing demands for innovative methods to improve energy efficiency and to prolong the network lifetime. Clustering is considered as an efficient topology control methods in wireless sensor networks, which can increase network scalability and lifetime. This paper presents a method, IP2P K-means – Improved P2P K-means, which uses efficient leveling in clustering approach, reduces false labeling and restricts the necessary communication among various sensors, which obviously saves more energy. The proposed method is examined in Network Simulator Ver.2 (NS2 and the preliminary results show that the algorithm works effectively and relatively more precisely.

  18. Predicting the Impact of Measures Against P2P Networks on the Transient Behaviors

    Altman, Eitan; Shwartz, Adam; Xu, Yuedong


    The paper has two objectives. The first is to study rigorously the transient behavior of some P2P networks where information is replicated and disseminated according to an epidemic type dynamics. The second is to use the insight gained in order to predict how efficient are measures taken against peer to peer networks. We first study a model which extends a classical epidemic model to characterize the peer to peer swarms in the presence of free riding peers. We then study a second model that a peer initiates a contact with another peer chosen randomly. In both cases, the network is shown to have a phase transition: a small change in the parameters causes a large change in the behavior of the network. We show in particular how the phase transition affects measures that content provider networks may take against P2P networks that distribute non-authorized music or books, and what is the efficiency of counter-measures.

  19. Proactive Uniform Data Replication by Density Estimation in Apollonian P2P Networks

    Bonnel, Nicolas; Ménier, Gildas; Marteau, Pierre-François

    We propose a data replication scheme on a random apollonian P2P overlay that benefits from the small world and scale free properties. The proposed algorithm features a replica density estimation and a space filling mechanism designed to avoid redundant messages. Not only it provides uniform replication of the data stored into the network but it also improves on classical flooding approaches by removing any redundancy. This last property is obtained at the cost of maintaining a random apollonian overlay. Thanks to the small world and scale free properties of the random apollonian P2P overlay, the search efficiency of the space filling tree algorithm we propose has comparable performances with the classical flooding algorithm on a random network.

  20. A hierarchical P2P overlay network for interest-based media contents lookup

    Lee, HyunRyong; Kim, JongWon


    We propose a P2P (peer-to-peer) overlay architecture, called IGN (interest grouping network), for contents lookup in the DHC (digital home community), which aims to provide a formalized home-network-extended construction of current P2P file sharing community. The IGN utilizes the Chord and de Bruijn graph for its hierarchical overlay network construction. By combining two schemes and by inheriting its features, the IGN efficiently supports contents lookup. More specifically, by introducing metadata-based lookup keyword, the IGN offers detailed contents lookup that can reflect the user interests. Moreover, the IGN tries to reflect home network environments of DHC by utilizing HG (home gateway) of each home network as a participating node of the IGN. Through experimental and analysis results, we show that the IGN is more efficient than Chord, a well-known DHT (distributed hash table)-based lookup protocol.

  1. Theoretical Studies of the Role of Vibrational Excitation on the Dynamics of the Hydrogen-Transfer Reaction of F(^2P) + HCl → FH + Cl({^2}P)

    Ray, Sara E.; Vissers, Gé W. M.; McCoy, Anne B.


    Hydrogen-transfer reactions are probed through vibrational excitation of the HCl bond in the pre-reactive F\\cdotsHCl complex. Such open-shell species provide a challenge for quantum dynamical calculations due to the need to take into account multiple potential energy surfaces to accurately describe the system.A three-dimensional, fully-coupled potential energy surface has been constructed based on electronic energies calculated at the multireference configuration interaction+Davidson correction (MRCI+Q) level of theory with an aug-cc-pVnZ (n=2,3,4) basis. Spin orbit calculations have also been included. Here we present the results of time-dependent quantum wave packet calculations on the asymmetric hydrogen-transfer reaction of F(^2P) + HCl. In these calculations, the reaction is initiated by vibrationally exciting the HCl stretching motion in the pre-reactive F\\cdotsHCl complex. The wave packet is propagated on the coupled potential energy surfaces. Product state distributions were calculated for reactions initiated in the first three vibrationally excited states of HCl, v=1-3. M. P. Deskevich, M. Y. Hayes, K. Takahashi, R. T. Skodje, and D. J. Nesbitt J. Chem. Phys. 124 (22) 224303 (2006) M. P. Deskevich and D. J. Nesbitt private communication(2007)

  2. Microstructure and Wear Behaviors of In-situ Al2O3p/7075 Composites%原位Al2O3P/7075复合材料微观组织与磨损行为

    刘慧敏; 杨树青; 许萍; 李进福


    采用原位反应近液相线铸造法制备具有不同质量分数的Al2O3P/7075复合材料,并对其进行干滑动磨损实验研究,通过OM,SEM,TEM等材料分析方法测试了材料的微观组织和磨损表面形貌.结果表明,原位Al2O3颗粒对7075铝合金的晶粒组织有明显细化效果,Al2O3P/7075复合材料的耐磨性比基体7075铝合金有明显的提高.原因是原位合成的复合材料界面结合良好,原位Al2O3颗粒在摩擦过程中起着抑制金属流动和支撑的双重作用.磨损表面形貌显示,原位Al2O3颗粒的加入,使磨损机制由黏着磨损变为磨粒磨损,从而改善了材料的耐磨性.%The Al2O3P/7075 Al composites were synthesized by in-situ reaction near-liquidus casting. The microstructure and dry sliding wear behavior of the prepared composites were analyzed using OM, SEM and TEM as well as wear friction testing. The results reveal that in-situ Al2O3 particle with average size of approximately <1μm is uniformly distributed in the matrix, which exhibits desirable refining effects on microstructure of 7075 Al alloy. The wear behavior of the composites is greatly superior to that of the matrix, which is attributed to the grain refining and formation of a compact interface of Al2O3P/7075 Al composites. In addition, in-situ Al2O3 particles exhibit coupling effects of inhibiting metal flow and bearing some load in process of friction. The wear mechanism of the matrix 7075A1 alloy is adhesive wear, while the wear surface of Al2O3p/7075 Al composites is superior to that of the matrix 7075 Al alloy. The wear mechanism of Al2O3P/7075 Al composites is abrasive wear, and wear resistance of Al2O3P/7075 Al is improved.

  3. Protocols for Bio-Inspired Resource Discovery and Erasure Coded Replication in P2P Networks

    Thampi, Sabu M


    Efficient resource discovery and availability improvement are very important issues in unstructured P2P networks. In this paper, a bio-inspired resource discovery scheme inspired by the principle of elephants migration is proposed. A replication scheme based on Q-learning and erasure codes is also introduced. Simulation results show that the proposed schemes significantly increases query success rate and availability, and reduces the network traffic as the resources are effectively distributed to well-performing nodes.

  4. A Distributed Architecture of Video Conference Using P2P Technology

    Xiaoyan Yu; Zhenyu Yu


    Most of the traditional video conferencing are standard SIP or H.323 architecture. There will be a single point of performance bottlenecks if large-scale video conference or a larger number of conferences emerge at the same time. In this paper, we proposed a new architecture of Multipoint Video Conference which can support a lot of meetings simultaneously using P2P and application multicast technology. In the side of server, the component called Media Control Server (MCS) is responsible for t...

  5. Personalized Trust Management for Open and Flat P2P Communities

    ZUO Min; LI Jian-hua


    A personalized trust management scheme is proposed to help peers build up trust between each other in open and flat P2P communities. This scheme totally abandons the attempt to achieve a global view. It evaluates trust from a subjective point of view and gives personalized decision support to each peer. Simulation experiments prove its three advantages: free of central control, stronger immunity to misleading recommendations, and limited traffic overload.

  6. Derivatives of benzimidazole: vasodilator activity of 2-(p-chloro-alpha-hydroxybenzyl)-benzimidazole hydrochloride. Preliminary study.

    Demenge, P; Carraz, G; Luu Duc, C; Silice, C


    The effects of 2-(p-chloro-alpha-hydroxybenzyl)-benzimidazole hydrochloride (HBBPC) have been studied in the rabbit and rat. Most of these studies were performed comparatively with reference vasodilators and papaverine. HBBPC vasodilator activity is nearly the same as that of papaverine in the isolated rabbit ear. The characteristic of the vasoactive action of HBBPC seems to reside in its duration. The mechanism of action of HBBPC seems of peripheral type, that is to say it acts on the vascular smooth muscle.

  7. Hybrid information retrieval policies based on cooperative cache in mobile P2P networks

    Quanqing XU; Hengtao SHEN; Zaiben CHEN; Bin CUI; Xiaofang ZHOU; Yafei DAI


    The concept of Peer-to-Peer (P2P) has been in-troduced into mobile networks, which has led to the emer-gence of mobile P2P networks, and originated potential ap-plications in many fields. However, mobile P2P networks are subject to the limitations of transmission range, and highly dynamic and unpredictable network topology, giving rise to many new challenges for efficient information retrieval. In this paper, we propose an automatic and economical hybrid information retrieval approach based on cooperative cache. In this method, the region covered by a mobile P2P network is partitioned into subregions, each of which is identified by a unique ID and known to all peers. All the subregions then constitute a mobile Kademlia (MKad) network. The pro-posed hybrid retrieval approach aims to utilize the flooding-based and Distributed Hash Table (DHT)-based schemes in MKad for indexing and searching according to the designed utility functions. To further facilitate information retrieval, we present an effective cache update method by considering all relevant factors. At the same time, the combination of two different methods for cache update is also introduced. One of them is pull based on time stamp including two different pulls: an on-demand pull and a periodical pull, and the other is a push strategy using update records. Furthermore, we provide detailed mathematical analysis on the cache hit ratio of our approach. Simulation experiments in NS-2 showed that the proposed approach is more accurate and efficient than the existing methods.

  8. Novel preparation of highly dispersed Ni2P embedded in carbon framework and its improved catalytic performance

    Wang, Shan; Wang, Kang; Wang, Xitao


    Highly dispersed Ni2P embedded in carbon framework with different phosphidation temperature was prepared through carbonizing Ni-alginate gel and followed by phosphidation with PPh3 in liquid phase. The significant effects of phosphidation temperature on Ni2P particle size and catalytic properties for isobutane dehydrogenation to isobutene were investigated. The results showed that Ni2P catalyst derived from the Ni-alginate gel (Ni2P-ADC), consisting of Ni2P particles embedded in carbon walls, possessed smaller particle size and more active site compared with Ni2P catalyst supported on active carbon (Ni2P/AC) prepared by impregnation method. The Ni2P-ADC catalyst phosphorized at 578 K for 3 h exhibited the highest catalytic performance, with the corresponding selectivity of isobutene approaching 89% and conversion approaching 15% after reaction for 4.5 h at 833 K, whereas Ni2P/AC catalyst prepared by impregnation method displays a much lower catalytic activity. The improved catalytic performance of the Ni2P-ADC can be ascribed to the smaller and highly dispersed Ni2P particles incorporated into carbon framework resulting from Ni-alginate gel.

  9. A Prediction System Using a P2P Overlay Network for a Bus Arrival System

    Ssu-Hsuan Lu


    Full Text Available Along with the evolution of times and the surge of metropolitan populations, government agencies often promote the construction of public transport. Unlike rail transportation or rapid transit systems, it is often difficult to estimate the vehicle arrival times at each station in a bus transportation system due to metropolitan transportation congestion. Traffic status is often monitored using wireless sensor networks (WSNs. However, WSNs are always separated from one another spatially. Recent studies have considered the connection of multiple sensor networks. This study considers a combination view of peer-to-peer (P2P overlay networks and WSN architecture to predict bus arrival times. Each bus station, which is also a P2P overlay peer, is connected in a P2P overlay network. A sensor installed in each bus can receive data via peers to obtain the moving speed of a bus. Then, each peer can exchange its data to predict bus arrival times at bus stations. This method can considerably increase the accuracy with which bus arrival times can be predicted and can provide traffic status with high precision. Furthermore, these data can also be used to plan new bus routes according to the information gathered.

  10. Design and Implementation Aspects of a novel Java P2P Simulator with GUI

    Chrissikopoulos, V; Sakkopoulos, E; Sioutas, S


    Peer-to-peer networks consist of thousands or millions of nodes that might join and leave arbitrarily. The evaluation of new protocols in real environments is many times practically impossible, especially at design and testing stages. The purpose of this paper is to describe the implementation aspects of a new Java based P2P simulator that has been developed to support scalability in the evaluation of such P2P dynamic environments. Evolving the functionality presented by previous solutions, we provide a friendly graphical user interface through which the high-level theoretic researcher/designer of a P2P system can easily construct an overlay with the desirable number of nodes and evaluate its operations using a number of key distributions. Furthermore, the simulator has built-in ability to produce statistics about the distributed structure. Emphasis was given to the parametrical configuration of the simulator. As a result the developed tool can be utilized in the simulation and evaluation procedures of a vari...

  11. Exploring highly porous Co2P nanowire arrays for electrochemical energy storage

    Chen, Minghua; Zhou, Weiwei; Qi, Meili; Yin, Jinghua; Xia, Xinhui; Chen, Qingguo


    Controllable synthesis of mesoporous conductive metal phosphide nanowire arrays is critical for developing highly-active electrodes of alkaline batteries. Herein we develop a simple combined strategy for rational synthesis of mesoporous Co2P nanowire arrays by hydrothermal-phosphorization method. Free-standing mesoporous Co2P nanowires consisting of interconnected nanoparticles of 10-20 nm grow vertically to the substrate forming arrays. High electrical conductivity and large porosity are obtained in the arrays architecture. When characterized as the cathode of high-rate alkaline batteries, the designed Co2P nanowire arrays are proven with good electrochemical performance with a large capacity (133 mAh g-1 at 1 A g-1), stable cycling life with a capacity retention of almost 100% after 5000 cycles at 10 A g-1 owing to the mesoporous nanowire structure with short ion/electron transport path. Our synthetic approach can be useful for construction of other porous metal phosphide arrays for energy storage and conversion.

  12. The PeerBehavior Model based on Continuous Behavioral Observation P2P Network Neighbors

    Xianwen Wu


    Full Text Available Honor-based trust mechanism is an important means to evaluate the behavior of the P2P network node, and it is used to ensure the health of the P2P network application. Trust mechanisms need to evaluate a node to other node local trust value and local trust value because they do not consider the policy node and human evaluation error of two important factors. The calculation is difficult to accurately reflect the characteristics of the nodes of the network. Evaluation models of the behavior of a P2P network neighbor. The PeerBehavior model has used a deterministic finite state machine (DFA depicts the continuous behavior of the neighbor state changes cause a negative evaluation of any continuous behavior by focusing neighbors almost died, both able to more accurately discover policies node in the network, but also be able to tolerate a certain degree of human evaluation error simulation experiment showed that this model was significantly improve the accuracy of the local trust value, and reducing the estimation error of the global trust value, was significantly superior to the calculation method of the current value of the other local trust


    Lucie Widowati


    Full Text Available Teratogenic test has been performed on sweet stevia as product of B2P2TO2T cultivation at  rats (Rattus novergicus pregnant female  Wistar. Stevia sweet was administered orally at a dose of 360, 120 and 40 mg/kg bw, volume 1 ml/100g bw per day during organogenesis period, on the day of pregnancy to the 6th until 15th. During the test, test animals were observed two times daily with the distance of six hours against the toxicity symptoms such as changes in skin, hair, eyes and mucous membranes, bleeding. Animals that experienced abortion, premature birth or death during the trial period were sacrificed and observed immediately with microscopic technic. At 20th day of pregnancy all of the pregnant rat dissected, and put out the fetuses  from the mother's and observated the health conditions in general and  the whole of mothers reproductive systems of fetuses, the outer fetal malformation and soft tissue system of fetal. The conclusion were  sweet stevia  of B2P2TO2T  no worse effect on the mother rats, fetal body weight and morphology of mother rats and the fetus, does not affect the process of development of fetal soft tissue, and does not affect the development of fetal skeleton. Generally the B2P2TO2T stevia sweet substances safe for used, does not cause teratogenic effects.

  14. K2P$^2$: Reduced data from campaigns 0-4 of the K2 mission

    Handberg, R


    Context: After the loss of a second reaction wheel the Kepler mission was redesigned as the K2 mission, pointing towards the ecliptic and delivering data for new fields approximately every 80 days. The steady flow of data obtained with a reduced pointing stability calls for dedicated pipelines for extracting light curves and correcting these for use in, e.g., asteroseismic analysis. Aims: We provide corrected light curves for the K2 fields observed until now (campaigns 0-4), and provide a comparison with other pipelines for K2 data extraction/correction. Methods: Raw light curves are extracted from K2 pixel data using the "K2-pixel-photometry" (K2P$^2$) pipeline, and corrected using the KASOC filter. Results: The use of K2P$^2$ allows for the extraction of the order of 90.000 targets in addition to 70.000 targets proposed by the community - for these, other pipelines provide no data. We find that K2P$^2$ in general performs as well as, or better than, other pipelines for the tested metrics of photometric qual...

  15. Agreement Maintenance Based on Schema and Ontology Change in P2P Environment

    Banowosari, L Y; Mutiara, A B


    This paper is concern about developing a semantic agreement maintenance method based on semantic distance by calculating the change of local schema or ontology. This approach is important in dynamic and autonomous environment, in which the current approach assumed that agreement or mapping in static environment. The contribution of this research is to develop a framework based on semantic agreement maintenance approach for P2P environment. This framework based on two level hybrid P2P model architecture, which consist of two peer type: (1) super peer that use to register and manage the other peers, and (2) simple peer, as a simple peer, it exports and shares its contents with others. This research develop a model to maintain the semantic agreement in P2P environment, so the current approach which does not have the mechanism to know the change, since it assumed that ontology and local schema are in the static condition, and it is different in dynamic condition. The main issues are how to calculate the change of...

  16. Optimally Efficient Prefix Search and Multicast in Structured P2P Networks

    Bradler, Dirk; Muehlhaeuser, Max


    Searching in P2P networks is fundamental to all overlay networks. P2P networks based on Distributed Hash Tables (DHT) are optimized for single key lookups, whereas unstructured networks offer more complex queries at the cost of increased traffic and uncertain success rates. Our Distributed Tree Construction (DTC) approach enables structured P2P networks to perform prefix search, range queries, and multicast in an optimal way. It achieves this by creating a spanning tree over the peers in the search area, using only information available locally on each peer. Because DTC creates a spanning tree, it can query all the peers in the search area with a minimal number of messages. Furthermore, we show that the tree depth has the same upper bound as a regular DHT lookup which in turn guarantees fast and responsive runtime behavior. By placing objects with a region quadtree, we can perform a prefix search or a range query in a freely selectable area of the DHT. Our DTC algorithm is DHT-agnostic and works with most exi...

  17. SKIP:an efficient search mechanism in unstructured P2P networks

    SHEN Wen-wu; SU Sen; SHUANG Kai; YANG Fang-chun


    Search efficiency and accuracy of resource are important considerations for search algorithm in peer-to-peer(P2P)network.Most search algorithms use flooding among neighbor nodes to search relevant resource.However,this usually causes great amount of redundant messages,which results in high search costs and low search precision.In this paper,we use vector space model(VSM)and relevance ranking algorithms to construct overlay network,and a novel search mechanism search with K-iteration preference(SKIP)based on semantic group for P2P networks is proposed to efficiently solve these problems.The key idea of SKIP is to reorder the semantic neighbors of nodes according to relevant scores and to utilize preference selection during the process of query.We analysis and implement the scheme and reveal that the SKIP provides a low overhead on topology maintenance,which can be effectively used in P2P searching and verify it outperformanee in higher precision and lower search cost by comparing with current semantic-based searching mechanism gnutella-like efficient searching system(GES).

  18. Hybrid CDN structure with a P2P based streaming protocol

    Pushp, Saumay


    Over the last decade, internet has seen an exponential increase in its growth.With more and more people using it, efficient data delivery over the internet has become a key issue. Peer-to-peer (P2P)/seed sharing based networks have several desirable features for content distribution, such as low costs, scalability, and fault tolerance. While the invention of each of such specialized systems has improved the user experience, some fundamental shortcomings of these systems have often been neglected. These shortcomings of content distribution systems have become severe bottlenecks in scalability of the internet.In order to combine the desired features of classical Content Distribution Networks (CDNs) and P2P/seed sharing based networks, we propose a hybrid CDN structure with a P2P/seed sharing based streaming protocol in the access network . In this work, we focus on the problem of data redundancy (at each node) and show how severely it impacts the network economics and the experience of end-user and hence leads ...

  19. SiMPSON: Efficient Similarity Search in Metric Spaces over P2P Structured Overlay Networks

    Vu, Quang Hieu; Lupu, Mihai; Wu, Sai

    Similarity search in metric spaces over centralized systems has been significantly studied in the database research community. However, not so much work has been done in the context of P2P networks. This paper introduces SiMPSON: a P2P system supporting similarity search in metric spaces. The aim is to answer queries faster and using less resources than existing systems. For this, each peer first clusters its own data using any off-the-shelf clustering algorithms. Then, the resulting clusters are mapped to one-dimensional values. Finally, these one-dimensional values are indexed into a structured P2P overlay. Our method slightly increases the indexing overhead, but allows us to greatly reduce the number of peers and messages involved in query processing: we trade a small amount of overhead in the data publishing process for a substantial reduction of costs in the querying phase. Based on this architecture, we propose algorithms for processing range and kNN queries. Extensive experimental results validate the claims of efficiency and effectiveness of SiMPSON.

  20. Fault Tolerant PLBGSA: Precedence Level Based Genetic Scheduling Algorithm for P2P Grid

    Piyush Chauhan


    Full Text Available Due to monetary limitation, small organizations cannot afford high end supercomputers to solve highly complex tasks. P2P (peer to peer grid computing is being used nowadays to break complex task into subtasks in order to solve them on different grid resources. Workflows are used to represent these complex tasks. Finishing such complex task in a P2P grid requires scheduling subtasks of workflow in an optimized manner. Several factors play their part in scheduling decisions. The genetic algorithm is very useful in scheduling DAG (directed acyclic graph based task. Benefit of a genetic algorithm is that it takes into consideration multiple criteria while scheduling. In this paper, we have proposed a precedence level based genetic algorithm (PLBGSA, which yields schedules for workflows in a decentralized fashion. PLBGSA is compared with existing genetic algorithm based scheduling techniques. Fault tolerance is a desirable trait of a P2P grid scheduling algorithm due to the untrustworthy nature of grid resources. PLBGSA handles faults efficiently.

  1. Relativistic calculations of 3s2 1S0-3s3p 1P1 and 3s2 1S0-3s3p 3P1,2 transition probabilities in the Mg isoelectronic sequence

    Cheng Cheng; Gao Xiang; Qing Bo; Zhang Xiao-Le; Li Jia-Ming


    Using the multi-configuration Dirac-Fock self-consistent field method and the relativistic configuration-interaction method, calculations of transition energies, oscillator strengths and rates are performed for the 3s2 1S0-3s3p 1P1 spinallowed transition, 3s2 1S0-3s3p 3P1,2 intercombination and magnetic quadrupole transition in the Mg isoelectronic sequence (Mg Ⅰ, Al Ⅱ, Si ⅢⅢ, P Ⅳ and S Ⅴ). Electron correlations are treated adequately, including intravalence electron correlations. The influence of the Breit interaction on oscillator strengths and transition energies are investigated. Quantum electrodynamics corrections are added as corrections. The calculation results are found to be in good agreement with the experimental data and other theoretical calculations.

  2. Radiative rates for E1, E2, M1, and M2 transitions among the 3s$^2$3p$^5$, 3s3p$^6$, and 3s$^2$3p$^4$3d configurations of Cl-like W LVIII

    Aggarwal, K M


    We report calculations of energy levels, radiative decay rates, and lifetimes for transitions among the 3s$^2$3p$^5$, 3s3p$^6$, and 3s$^2$3p$^4$3d configurations of Cl-like W LVIII. The general-purpose relativistic atomic structure package (GRASP) has been adopted for our calculations. Comparisons are made with the most recent results of Mohan et al. [Can. J. Phys. {\\bf 92} (2014) xxx] and discrepancies in lifetimes are noted, up to four orders of magnitude in some instances. Our energy levels are estimated to be accurate to better than 0.5\\%, whereas results for radiative rates and lifetimes should be accurate to better than 20\\%.

  3. Inner-Shell Excitations of 2p Electrons of Argon Investigated by Fast Electron Impact with High Resolution

    REN Lin-Mao; WANG You-Yan; LI Dong-Dong; YUAN Zhen-Sheng; ZHU Lin-Fan


    @@ Electron energy loss spectra of inner-shell excitations of 2p electrons of argon are measured at an incident electron energy of 2500eV and scattering angles of 0° and 4°.The dipole-forbidden transitions of 2p3/2-14p and 2p3/2-15p are observed in the measured spectra and assigned based on the calculations of the Cowan code.The positions and line widths for the excitations of 2p3/2-1nl and 2p1/2-1nl(n ≤ 5) of argon are determined.The present results show that the line widths of the electric quadrupole transitions of 2p3/2-14p[5/2 + 3/2]2 and the electric monopole one of 2p3/2-14p[1/2]0 are less than those of the dipole-allowed transitions.%Electron energy loss spectra of inner-shell excitations of 2p electrons of argon are measured at an incident electron energy of 2500eV and scattering angles of 0° and 4°. The dipole-forbidden transitions of 2p-13/24p and 2p-13/25p are observed in the measured spectra and assigned based on the calculations of the Cowan code. The positions and line widths for the excitations of 2p-13/2nl and 2p-11/2nl(n ≤ 5) of argon are determined. The present results show that the line widths of the electric quadrupole transitions of 2p-13/24p[5/2 + 3/2]2 and the electric monopole one of 2p-13/2 4p[1/2]o are less than those of the dipole-allowed transitions.

  4. Absence of mutations in the coding sequence of the potential tumor suppressor 3pK in metastatic melanoma

    Houben Roland


    Full Text Available Abstract Background Activation of Ras or Raf contributes to tumorigenesis of melanoma. However, constitutive Raf activation is also a characteristic of the majority of benign melanocytic nevi and high intensity signaling of either Ras or Raf was found to induce growth inhibition and senescence rather than transformation. Since the chromosome 3p kinase (3pK is a target of the Ras/Raf/Mek/Erk signaling pathway which antagonizes the function of the oncogene and anti-differentiation factor Bmi-1, 3pK may function as a tumor suppressor in tumors with constitutive Ras/Raf activation. Consequently, we tested whether inactivating 3pK mutations are present in melanoma. Methods 30 metastatic melanoma samples, which were positive for activating mutations of either BRaf or NRas, were analyzed for possible mutations in the 3pk gene. The 10 coding exons and their flanking intron sequences were amplified by PCR and direct sequencing of the PCR products was performed. Results This analysis revealed that besides the presence of some single nucleotide polymorphisms in the 3pk gene, we could not detect any possible loss of function mutation in any of these 30 metastatic melanoma samples selected for the presence of activating mutations within the Ras/Raf/Mek/Erk signaling pathway. Conclusion Hence, in melanoma with constitutively active Ras/Raf inactivating mutations within the 3pk gene do not contribute to the oncogenic phenotype of this highly malignant tumor.

  5. $\\beta$3$p$-spectroscopy and proton-$\\gamma$ width determination in the decay of $^{31}$Ar

    We propose to perform a detailed study of the $\\beta$-decay of the dripline nucleus $^{31}$Ar. This will allow a detailed study of the $\\beta$-delayed 3$p$-decay as well as provide important information on the resonances of $^{30}$S and $^{29}$P, in particular the ratio between the $p$- and $\\gamma$- partial widths relevant for astrophysics.

  6. Reduced miR-659-3p levels correlate with progranulin increase in hypoxic conditions: implications for frontotemporal dementia.

    Paola ePiscopo


    Full Text Available Progranulin (PGRN is a secreted protein expressed ubiquitously throughout the body, including the brain, where it localizes in neurons and activated microglia. Loss-of-function mutations in the GRN gene are an important cause of familial Frontotemporal Lobar Degeneration (FTLD. PGRN has a neurotrophic and anti-inflammatory activity, and it is neuroprotective in several injury conditions, such as oxygen or glucose deprivation, oxidative injury, and hypoxic stress. Indeed, we have previously demonstrated that hypoxia induces the up-regulation of GRN transcripts. Several studies have shown microRNAs involvement in hypoxia. Moreover, in FTLD patients with a genetic variant of GRN (rs5848, the reinforcement of miR-659-3p binding site has been suggested to be a risk factor. Here, we report that miR-659-3p interacts directly with GRN 3’UTR as shown by luciferase assay in HeLa cells and ELISA and Western Blot analysis in HeLa and Kelly cells. Moreover, we demonstrate the physical binding between GRN mRNA and miR-659-3p employing a miRNA capture-affinity technology in SK-N-BE and Kelly cells. In order to study miRNAs involvement in hypoxia-mediated up-regulation of GRN, we evaluated miR-659-3p levels in SK-N-BE cells after 24h of hypoxic treatment, finding them inversely correlated to GRN transcripts. Furthermore, we analyzed an animal model of asphyxia, finding that GRN mRNA levels increased at post-natal day (pnd 1 and pnd 4 in rat cortices subjected to asphyxia in comparison to control rats and miR-659-3p decreased at pnd 4 just when GRN reached the highest levels. Our results demonstrate the interaction between miR-659-3p and GRN transcript and the involvement of miR-659-3p in GRN up-regulation mediated by hypoxic/ischemic insults.

  7. Evidence that intramolecular interactions are involved in masking the activation domain of transcriptional activator Leu3p.

    Wang, D; Hu, Y; Zheng, F; Zhou, K; Kohlhaw, G B


    The Leu3 protein of Saccharomyces cerevisiae regulates the expression of genes involved in branched chain amino acid biosynthesis and in ammonia assimilation. It is modulated by alpha-isopropylmalate, an intermediate in leucine biosynthesis. In the presence of alpha-isopropylmalate, Leu3p is a transcriptional activator. In the absence of the signal molecule, the activation domain is masked, and Leu3p acts as a repressor. The recent discovery that Leu3p retains its regulatory properties when expressed in mammalian cells (Guo, H., and Kohlhaw, G. B. (1996) FEBS Lett. 390, 191-195) suggests that masking and unmasking of the activation domain occur without the participation of auxiliary proteins. Here we present experimental support for this notion and address the mechanism of masking. We show that modulation of Leu3p is exceedingly sensitive to mutations in the activation domain. An activation domain double mutant (D872N/D874N; designated Leu3-dd) was constructed that has the characteristics of a permanently masked activator. Using separately expressed segments containing either the DNA binding domain-middle region or the activation domain of wild type Leu3p (or Leu3-dd) in a modified yeast two-hybrid system, we provide direct evidence for alpha-isopropylmalate-dependent interaction between these segments. Finally, we use the phenotype of Leu3-dd-containing cells (slow growth in the absence of added leucine) to select for suppressor mutations that map to the middle region of Leu3-dd. The properties of nine such suppressors further support the idea that masking is an intramolecular process and suggest a means for mapping the surface involved in masking.

  8. MiR-466b-1-3p regulates P-glycoprotein expression in rat cerebral microvascular endothelial cells.

    Yang, Xiaobo; Ren, Weimin; Shao, Yiye; Chen, Yinghui


    Epilepsy is one of the most common neurological disorders, and approximately one-third of epilepsy cases are resistant to treatment with anti-epileptic drug (AED). P-glycoprotein (P-gp) is a multi-drug transporter that is thought to play a pivotal role in multiple drug resistance (MDR) in epilepsy. The regulatory mechanism of P-gp remains largely unknown; however, recent studies have demonstrated that microRNAs (miRNAs) may regulate the chemo-resistance mediated by P-gp. This study investigated the effect of specific miRNAs that regulate P-gp expression in rat cerebral microvascular endothelial cells (RCMECs). Primary cultures of RCMECs were treated with phenobarbital (PB) at various concentrations to induce P-gp overexpression. MiRNA microarrays were used to investigate the expression profiles of miRNAs in the resistant RCMECs induced by PB and corresponding non-resistant cells. Our data demonstrated decreased miR-466b-1-3p expression in the resistant cells compared with the non-resistant cells. Moreover, the recombinant RNA of 466b-1-3p (mimic) and the artificial antisense RNA of miR-466b-1-3p (inhibitor) were constructed and transfected into resistant RCMECs. The expression and function of P-gp were measured by Western blotting, quantitative real-time polymerase chain reaction (qRT-PCR) and flow cytometry using rhodamine efflux. The mRNA and protein levels of P-gp increased as the concentration of PB increased, whereas miR-466b-1-3p levels decreased with increasing PB concentrations (Pp mimic down-regulated P-gp expression, whereas the miR-466b-1-3p inhibitor up-regulated P-gp expression (Pp may regulate PB-induced P-gp expression in RCMECs.

  9. Role of miR-222-3p in c-Src-Mediated Regulation of Osteoclastogenesis

    Shinya Takigawa


    Full Text Available MicroRNAs (miRNAs are small non-coding RNAs that play a mostly post-transcriptional regulatory role in gene expression. Using RAW264.7 pre-osteoclast cells and genome-wide expression analysis, we identified a set of miRNAs that are involved in osteoclastogenesis. Based on in silico analysis, we specifically focused on miR-222-3p and evaluated its role in osteoclastogenesis. The results show that the inhibitor of miR-222-3p upregulated the mRNA levels of nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1 and tartrate-resistant acid phosphatase (TRAP, while its mimicking agent downregulated their mRNA levels. Western blot analysis showed that its inhibitor increased the protein levels of TRAP and cathepsin K, while its mimicking agent decreased their levels. Genome-wide mRNA expression analysis in the presence and absence of receptor activator of nuclear factor κ-B ligand (RANKL predicted c-Src as a potential regulatory target of miR-222-3p. Live cell imaging using a fluorescence resonance energy transfer (FRET technique revealed that miR-222-3p acted as an inhibitor of c-Src activity, and a partial silencing of c-Src suppressed RANKL-induced expression of TRAP and cathepsin K, as well as the number of multi-nucleated osteoclasts and their pit formation. Collectively, the study herein demonstrates that miR-222-3p serves as an inhibitor of osteoclastogenesis and c-Src mediates its inhibition of cathepsin K and TRAP.

  10. Affinity Purification and Characterization of a G-Protein Coupled Receptor, Saccharomyces cerevisiae Ste2p

    Lee, Byung-Kwon [University of Tennessee, Knoxville (UTK); Jung, Kyung-Sik [University of Tennessee, Knoxville (UTK); Son, Cagdas D [ORNL; Kim, Heejung [University of Tennessee, Knoxville (UTK); Verberkmoes, Nathan C [ORNL; Arshava, Boris [College of Staten Island; Naider, Fred [College of Staten Island; Becker, Jeffrey Marvin [ORNL


    We present a rare example of a biologically active G protein coupled receptor (GPCR) whose purity and identity were verified by mass spectrometry after being purified to near homogeneity from its native system. An overexpression vector was constructed to encode the Saccharomyces cerevisiae GPCR -factor receptor (Ste2p, the STE2 gene product) containing a 9-amino acid sequence of rhodopsin that served as an epitope/affinity tag. In the construct, two glycosylation sites and two cysteine residues were removed to aid future structural and functional studies. The receptor was expressed in yeast cells and was detected as a single band in a western blot indicating the absence of glycosylation. Tests of the epitope-tagged, mutated receptor showed it maintained its full biological activity. For extraction of Ste2p, yeast membranes were solubilized with 0.5 % n-dodecyl maltoside (DM). Approximately 120 g of purified -factor receptor was obtained per liter of culture by single-step affinity chromatography using a monoclonal antibody to the rhodopsin epitope. The binding affinity (Kd) of the purified -factor receptor in DM micelles was 28 nM as compared to Kd = 12.7 nM for Ste2p in cell membranes, and approximately 40 % of the purified receptor was correctly folded as judged by ligand saturation binding. About 50 % of the receptor sequence was retrieved from MALDITOF and nanospray mass spectrometry after CNBr digestion of the purified receptor. The methods described will enable structural studies of the -factor receptor and may provide an efficient technique to purify other GPCRs that have been functionally expressed in yeast.

  11. Transport and electroluminescence mechanism in Au/(Si/SiO2)/P-Si film

    ZHANG Kai-biao; MA Shu-yi; MA Zi-jun; CHEN Hai-xia


    The samples of Au/(Si/SiO2)/p-Si structure were fabricated by using the R.F magnetron sputtering technique.Its carrier transport and electroluminescence mechanism were studied from the I-V curves and EL spectra by using the Configuration Coordinate as a theoretical model.The result indicates that there are two defect centers in SiO2 films.The electron in Au and the hole in p-Si went into SiO2 film by the Fowler-Nordheim tunneling model at a high bias voltage and recombined through these defect centers in SiO2 film.

  12. Emergence of Financial Intermediaries in Electronic Markets: The Case of Online P2P Lending

    Sven C. Berger


    Full Text Available We analyze the role of intermediaries in electronic markets using detailed data of more than 14,000 originated loans on an electronic P2P (peer-to-peer lending platform. In such an electronic credit market, lenders bid to supply a private loan. Screening of potential borrowers and the monitoring of loan repayment can be delegated to designated group leaders. We find that these market participants act as financial intermediaries and significantly improve borrowers' credit conditions by reducing information asymmetries, predominantly for borrowers with less attractive risk characteristics. Our findings may be surprising given the replacement of a bank by an electronic marketplace.

  13. Observation of a Quasideuteron Component in the Reaction 12C(π+, 2p)

    Altman, A.; Piasetzky, E.; Lichtenstadt, J.; Yavin, A. I.; Ashery, D.; Powers, R. J.; Bertl, W.; Felawka, L.; Walter, H. K.; Winter, R. G.; Pluym, J. V. D.


    The reaction 12C(π+, 2p) was studied at 165 and 245 MeV over a broad kinematic range by coincidence measurement of the outgoing protons. The p-p angular correlations and proton energy spectra allow an identification of the "quasideuteron" component of the reaction. The angular distribution of this component has a shape similar to that of the π+d-->pp reaction. It is found that one-step quasideuteron absorption accounts for at most 25% of the total absorption cross section.

  14. Melting of Al Induced by Laser Excitation of 2p Holes

    Rosandi, Y.; Cheenicode Kabeer, F.; Cherednikov, Y.; Zijlstra, E.; Garcia, M; Inogamov, N.; Urbassek, H.


    Novel photon sources—such as XUV- or X-ray lasers—allow to selectively excite core excitations in materials. We study the response of a simple metal, Al, to the excitation of 2p holes using molecular dynamics simulations. During the lifetime of the holes, the interatomic interactions in the slab are changed; we calculate these using WIEN2k. We find that the melting dynamics after core-hole excitation is dominated by classical electron–phonon dynamics. The effects of the changed potential surf...

  15. Nuclear transparency in 90 °c.m. quasielastic A(p,2p) reactions

    Aclander, J.; Alster, J.; Asryan, G.; Averiche, Y.; Barton, D. S.; Baturin, V.; Buktoyarova, N.; Bunce, G.; Carroll, A. S.; Christensen, N.; Courant, H.; Durrant, S.; Fang, G.; Gabriel, K.; Gushue, S.; Heller, K. J.; Heppelmann, S.; Kosonovsky, I.; Leksanov, A.; Makdisi, Y. I.; Malki, A.; Mardor, I.; Mardor, Y.; Marshak, M. L.; Martel, D.; Minina, E.; Minor, E.; Navon, I.; Nicholson, H.; Ogawa, A.; Panebratsev, Y.; Piasetzky, E.; Roser, T.; Russell, J. J.; Schetkovsky, A.; Shimanskiy, S.; Shupe, M. A.; Sutton, S.; Tanaka, M.; Tang, A.; Tsetkov, I.; Watson, J.; White, C.; Wu, J.-Y.; Zhalov, D.


    We summarize the results of two experimental programs at the Alternating Gradient Synchrotron of BNL to measure the nuclear transparency of nuclei measured in the A(p,2p) quasielastic scattering process near 90° in the pp center of mass. The incident momenta varied from 5.9 to 14.4 GeV/c , corresponding to 4.8< Q2 <12.7 (GeV/c)2 . Taking into account the motion of the target proton in the nucleus, the effective incident momenta extended from 5.0 to 15.8 GeV/c . First, we describe the measurements with the newer experiment, E850, which had more complete kinematic definition of quasielastic events. E850 covered a larger range of incident momenta, and thus provided more information regarding the nature of the energy dependence of the nuclear transparency. In E850 the angular dependence of the nuclear transparency near 90° and the nuclear transparency deuterons were studied. Second, we review the techniques used in an earlier experiment, E834, and show that the two experiments are consistent for the carbon data. E834 also determines the nuclear transparencies for lithium, aluminum, copper, and lead nuclei as well as for carbon. A determination of the ( π+ , π+ p) transparencies is also reported. We find for both E850 and E834 that the A(p,2p) nuclear transparency, unlike that for A(e, e' p) nuclear transparency, is incompatible with a constant value versus energy as predicted by Glauber calculations. The A(p,2p) nuclear transparency for carbon and aluminum increases by a factor of two between 5.9 and 9.5 GeV/c incident proton momentum. At its peak the A(p,2p) nuclear transparency is ˜80% of the constant A(e, e' p) nuclear transparency. Then the nuclear transparency falls back to a value at least as small as that at 5.9 GeV/c , and is compatible with the Glauber level again. This oscillating behavior is generally interpreted as an interplay between two components of the pN scattering amplitude; one short ranged and perturbative, and the other long ranged and

  16. Impact of lead ions on biosynthetic capacity of Streptomyces recifensis var. lyticus 2p-15 strain

    Т. P. Kilochok


    Full Text Available The influence of different concentrations of Pb ions on biosynthetical ability of Streptomyces recifensis var. lyticus 2P-15, which is the producer of compound complex of extracellular enzymes and growth stimulators, was studied. It has been showed, that Pb ions introduced in agar medium have had a stimulative effect on production of surface and depth mycelia. The Pb ions, which have been inoculated into liquid fermentative medium in concentration of 1,0–2,0 mg/l realized directed synthesis of bacterio- and proteolytic enzymes, had an influence on qualitative and quantitative composition of produced enzymes.

  17. Acoustic and elastic properties of Sn(2)P(2)S(6) crystals.

    Mys, O; Martynyuk-Lototska, I; Grabar, A; Vlokh, R


    We present the results concerned with acoustic and elastic properties of Sn(2)P(2)S(6) crystals. The complete matrices of elastic stiffness and compliance coefficients are determined in both the crystallographic coordinate system and the system associated with eigenvectors of the elastic stiffness tensor. The acoustic slowness surfaces are constructed and the propagation and polarization directions of the slowest acoustic waves promising for acousto-optic interactions are determined on this basis. The acoustic obliquity angle and the deviation of polarization of the acoustic waves from purely transverse or longitudinal states are quantitatively analysed.

  18. A P2P Service Discovery Strategy Based on Content Catalogues

    Lican Huang


    Full Text Available This paper presents a framework for distributed service discovery based on VIRGO P2P technologies. The services are classified as multi-layer, hierarchical catalogue domains according to their contents. The service providers, which have their own service registries such as UDDIs, register the services they provide and establish a virtual tree in a VIRGO network according to the domain of their service. The service location done by the proposed strategy is effective and guaranteed. This paper also discusses the primary implementation of service discovery based on Tomcat/Axis and jUDDI.

  19. Relativistic model of 2p-2h meson exchange currents in (anti)neutrino scattering

    Simo, I Ruiz; Barbaro, M B; De Pace, A; Caballero, J A; Donnelly, T W


    We develop a model of relativistic, charged meson-exchange currents (MEC) for neutrino-nucleus interactions. The two-body current is the sum of seagull, pion-in-flight, pion-pole and $\\Delta$-pole operators. These operators are obtained from the weak pion-production amplitudes for the nucleon derived in the non-linear $\\sigma$-model together with weak excitation of the $\\Delta(1232)$ resonance and its subsequent decay into $N\\pi$. With these currents we compute the five 2p-2h response functions contributing to $(\

  20. Towards Accurate Node-Based Detection of P2P Botnets

    Chunyong Yin


    Full Text Available Botnets are a serious security threat to the current Internet infrastructure. In this paper, we propose a novel direction for P2P botnet detection called node-based detection. This approach focuses on the network characteristics of individual nodes. Based on our model, we examine node’s flows and extract the useful features over a given time period. We have tested our approach on real-life data sets and achieved detection rates of 99-100% and low false positives rates of 0–2%. Comparison with other similar approaches on the same data sets shows that our approach outperforms the existing approaches.