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Sample records for 2p 3p 5q

  1. Overexpression of c-myc and loss of heterozigosity on 2p, 3p, 5q, 17p and 18q in sporadic colorectal carcinoma Sobreexpresión de c-myc y pérdida de heterozigosidad en 2p, 3p, 5q, 17p y 18q en carcinoma colorrectal esporádico

    Directory of Open Access Journals (Sweden)

    A. Sánchez-Pernaute

    2005-03-01

    Full Text Available Aim: the aim of the present study is to evaluate the prognostic influence of loss of heterozygosity on 2p, 3p, 5q, 17p and 18q, and c-myc overexpression on surgically treated sporadic colorectal carcinoma. Methods: tumor and non-tumor tissue samples from 153 patients were analyzed. Fifty-one percent of patients were male, and mean age in the series was 67 years. Tumors were located in the proximal colon in 37 cases, in the distal bowel in 37, and in the rectum in 79 patients. c-myc overexpression was studied by means of Northern blot analysis, and loss of heterozigosity through microsatellite analysis. Results: c-myc overexpression was detected in 25% of cases, and loss of heterozygosity in at least one of the studied regions in 48%. There was no association between clinical and pathologic features, and genetic alterations. The disease-free interval was significantly shorter for patients with both genetic alterations; the presence of both events was an independent prognostic factor for poor outcome in the multivariate analysis (RR: 4.34, p Objetivo: el objetivo del presente trabajo es evaluar la importancia pronóstica de la pérdida de heterozigosidad en las regiones 2p, 3p, 5q, 17p y 18q y de la sobreexpresión del gen c-myc en el carcinoma colorrectal esporádico, mediante el estudio de la supervivencia libre de enfermedad tras cirugía potencialmente curativa. Métodos: se han analizado muestras tumorales y no tumorales de mucosa colónica de 153 pacientes. El 51% de los pacientes eran varones y la edad media de la serie fue 67 años. Los tumores fueron proximales en 37 casos, distales en 37 y localizados en recto en 79. Se analizó la sobreexpresión del RNA de c-myc por Northern blot, y la presencia de pérdida de heterozigosidad en las diferentes regiones consideradas por análisis de microsatélites. Resultados: se detectó sobreexpresión de c-myc en el 25% de los casos, y pérdida de heterozigosidad en alguna de las regiones estudiadas

  2. Doubly excited 2s2p 1,3p1 resonances in photoionization of helium

    Institute of Scientific and Technical Information of China (English)

    Wan Jian-Jie; Dong Chen-Zhong

    2009-01-01

    The multi-configuration Dirac-Fock (MCDF) method is implemented to study doubly excited 2s2p 1,3P1 resonances of the helium atom and the interference between photoionization and photoexcitation autoionization processes.In order to reproduce the total photoionization sprectra,the excited energies from the ground ls2 1 S0 state to the doubly excited 2s2p 1'3P1 states and the relevant Auger decay rates and widths are calculated in detail. Furthermore,the interference profile determined by the so-called Fano parameters q and p2 is also reproduced. Good agreement is found between the present results and other available theoretical and experimental results. This indeed shows a promising way to investigate the Fano resonances in photoionization of atoms within the MCDF scheme,although there are some discrepancies in the present calculations of the 2s2p 3P1 state.

  3. Unified interpretation of Hund's first and second rules for 2p and 3p atoms.

    Science.gov (United States)

    Oyamada, Takayuki; Hongo, Kenta; Kawazoe, Yoshiyuki; Yasuhara, Hiroshi

    2010-10-28

    A unified interpretation of Hund's first and second rules for 2p (C, N, O) and 3p (Si, P, S) atoms is given by Hartree-Fock (HF) and multiconfiguration Hartree-Fock (MCHF) methods. Both methods exactly satisfy the virial theorem, in principle, which enables one to analyze individual components of the total energy E(=T+V(en)+V(ee)), where T, V(en), and V(ee) are the kinetic, the electron-nucleus attraction, and the electron-electron repulsion energies, respectively. The correct interpretation for each of the two rules can only be achieved under the condition of the virial theorem 2T+V=0 by investigating how V(en) and V(ee) interplay to attain the lower total potential energy V(=V(en)+V(ee)). The stabilization of the more stable states for all the 2p and 3p atoms is ascribed to a greater V(en) that is caused by contraction of the valence orbitals accompanied with slight expansion of the core orbitals. The contraction of the valence orbitals for the two rules is a consequence of reducing the Hartree screening of the nucleus at short interelectronic distances. The reduced screening in the first rule is due to a greater amount of Fermi hole contributions in the state with the highest total spin-angular momentum S. The reduced screening in the second rule is due to the fact that two valence electrons are more likely to be on opposite sides of the nucleus in the state with the highest total orbital-angular momentum L. For each of the two rules, the inclusion of correlation does not qualitatively change the HF interpretation, but HF overestimates the energy difference ∣ΔE∣ between two levels being compared. The magnitude of the correlation energy is significantly larger for the lower L states than for the higher L states since two valence electrons in the lower L states are less likely to be on opposite sides of the nucleus. The MCHF evaluation of ∣ΔE∣ is in excellent agreement with experiment. The present HF and MCHF calculations demonstrate the above statements

  4. Phospholipid flippases Lem3p-Dnf1p and Lem3p-Dnf2p are involved in the sorting of the tryptophan permease Tat2p in yeast.

    Science.gov (United States)

    Hachiro, Takeru; Yamamoto, Takaharu; Nakano, Kenji; Tanaka, Kazuma

    2013-02-01

    The type 4 P-type ATPases are flippases that generate phospholipid asymmetry in membranes. In budding yeast, heteromeric flippases, including Lem3p-Dnf1p and Lem3p-Dnf2p, translocate phospholipids to the cytoplasmic leaflet of membranes. Here, we report that Lem3p-Dnf1/2p are involved in transport of the tryptophan permease Tat2p to the plasma membrane. The lem3Δ mutant exhibited a tryptophan requirement due to the mislocalization of Tat2p to intracellular membranes. Tat2p was relocalized to the plasma membrane when trans-Golgi network (TGN)-to-endosome transport was inhibited. Inhibition of ubiquitination by mutations in ubiquitination machinery also rerouted Tat2p to the plasma membrane. Lem3p-Dnf1/2p are localized to endosomal/TGN membranes in addition to the plasma membrane. Endocytosis mutants, in which Lem3p-Dnf1/2p are sequestered to the plasma membrane, also exhibited the ubiquitination-dependent missorting of Tat2p. These results suggest that Tat2p is ubiquitinated at the TGN and missorted to the vacuolar pathway in the lem3Δ mutant. The NH(2)-terminal cytoplasmic region of Tat2p containing ubiquitination acceptor lysines interacted with liposomes containing acidic phospholipids, including phosphatidylserine. This interaction was abrogated by alanine substitution mutations in the basic amino acids downstream of the ubiquitination sites. Interestingly, a mutant Tat2p containing these substitutions was missorted in a ubiquitination-dependent manner. We propose the following model based on these results; Tat2p is not ubiquitinated when the NH(2)-terminal region is bound to membrane phospholipids, but if it dissociates from the membrane due to a low level of phosphatidylserine caused by perturbation of phospholipid asymmetry in the lem3Δ mutant, Tat2p is ubiquitinated and then transported from the TGN to the vacuole.

  5. Reactions of R(2)P-P(SiMe(3))Li with [(R'(3)P)(2)PtCl(2)]. A general and efficient entry to phosphanylphosphinidene complexes of platinum. Syntheses and structures of [(eta(2)-P=(i)Pr(2))Pt(p-Tol(3)P)(2)], [(eta(2)-P=(t)Bu(2))Pt(p-Tol(3)P)(2)], [{eta(2)-P=(N(i)Pr(2))(2)}Pt(p-Tol(3)P)(2)] and [{(Et(2)PhP)(2)Pt}(2)P(2)].

    Science.gov (United States)

    Domańska-Babul, Wioleta; Chojnacki, Jaroslaw; Matern, Eberhard; Pikies, Jerzy

    2009-01-07

    The reactions of lithium derivatives of diphosphanes R(2)P-P(SiMe(3))Li (R = (t)Bu, (i)Pr, Et(2)N and (i)Pr(2)N) with [(R'(3)P)(2)PtCl(2)] (R'(3)P = Et(3)P, Et(2)PhP, EtPh(2)P and p-Tol(3)P) proceed in a facile manner to afford side-on bonded phosphanylphosphinidene complexes of platinum [(eta(2)-P=R(2))Pt(PR'(3))(2)]. The related reactions of Ph(2)P-P(SiMe(3))Li with [(R'(3)P)(2)PtCl(2)] did not yield [(eta(2)-P=PPh(2))Pt(PR'(3))(2)] and resulted mainly in the formation of [{(R'(3)P)(2)Pt}(2)P(2)], Ph(2)P-PLi-PPh(2), (Me(3)Si)(2)PLi and (Me(3)Si)(3)P. Crystallographic data are reported for the compounds [(eta(2)-P=R(2))Pt(p-Tol(3)P)(2)] (R = (t)Bu, (i)Pr, ((i)Pr(2)N)(2)P) and for [{(Et(2)PhP)(2)Pt}(2)P(2)].

  6. Identification of a rare de novo three-way complex t(5;20;8(q31;p11.2;p21 with microdeletions on 5q31.2, 5q31.3, and 8p23.2 in a patient with hearing loss and global developmental delay: case report

    Directory of Open Access Journals (Sweden)

    Bejjani Bassem A

    2009-01-01

    Full Text Available Abstract Background Complex chromosome rearrangements (CCRs, which involve more than two breakpoints on two or more chromosomes, are uncommon occurrences. Although most CCRs appear balanced at the level of the light microscope, many demonstrate cryptic, submicroscopic imbalances at the translocation breakpoints. Results We report a female with hearing loss and global developmental delay with a complex three-way unbalanced translocation (5;20;8(q31;p11.2;p21 resulting in microdeletions on 5q31.2, 5q31.3, and 8p23.2 identified by karyotyping, microarray analysis and fluorescence in situ hybridization. Discussion The microdeletion of bands 8p23.2 may be associated with the hearing impairment. Furthermore, the characterization of this patient's chromosomal abnormalities demonstrates the importance of integrated technologies within contemporary cytogenetics laboratories.

  7. The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo.

    OpenAIRE

    Brown, J. T.; Bai, X.; Johnson, A. W.

    2000-01-01

    The yeast superkiller (SKI) genes were originally identified from mutations allowing increased production of killer toxin encoded by M "killer" virus, a satellite of the dsRNA virus L-A. XRN1 (SKI1) encodes a cytoplasmic 5'-exoribonuclease responsible for the majority of cytoplasmic RNA turnover, whereas SKI2, SKI3, and SKI8 are required for normal 3'-degradation of mRNA and for repression of translation of poly(A) minus RNA. Ski2p is a putative RNA helicase, Ski3p is a tetratricopeptide repe...

  8. Yeast Agp2p and Agp3p function as amino acid permeases in poor nutrient conditions.

    Science.gov (United States)

    Schreve, James L; Garrett, Jinnie M

    2004-01-16

    The gene AGP2 and the ORF YFL055w (here named AGP3) are classified as members of the yeast amino acid permease gene family. Analysis of the growth of multiply-mutant strains in which these genes are disrupted shows that both encode permeases capable of supplying branched chain, and other, amino acids as nitrogen source. Both Agp2p and Agp3p are low affinity permeases for leucine (Kmapp 0.2-0.5 mM) and are expressed at lower levels than other permeases on all media tested. Thus, it appears that these two permeases can function as low affinity, relatively non-specific, permeases with redundant functions in the cell. Transcription of AGP2 and AGP3 is very low but is increased in cells lacking other functional general amino acid permeases (Gap1p or Agp1p). These results suggest Agp2p and Agp3p function in amino acid transport when nitrogen sources are limiting and/or other permeases are inactive.

  9. 75 FR 23574 - Airworthiness Directives; CFM International, S.A. CFM56-5B1/P, -5B2/P, -5B3/P, -5B3/P1, -5B4/P...

    Science.gov (United States)

    2010-05-04

    ..., S.A. CFM56-5B1/P, - 5B2/P, -5B3/P, -5B3/P1, -5B4/P, -5B5/P, -5B6/P, -5B7/P, -5B8/P, -5B9/P, -5B1/2P... Docket Operations office between 9 a.m. and 5 p.m., Monday through Friday, except Federal holidays. The... CFM International, S.A. CFM56-5B1/P, - 5B2/P, -5B3/P, -5B3/P1, -5B4/P, -5B5/P,......

  10. Photoexcitation and photoionization from the 2p{sup 5}3p[5/2]{sub 2,3} levels in neon

    Energy Technology Data Exchange (ETDEWEB)

    Baig, M. A.; Bokhari, I. A.; Rafiq, M.; Kalyar, M. A.; Hussian, T.; Ali, Raheel; Piracha, N. K. [Atomic and Molecular Physics Laboratory, Department of Physics, Quaid-i-Azam University, 45320 Islamabad (Pakistan); Department of Physics, John Carroll University, 20700 N. Park Boulevard, University Heights, Ohio 44118 (United States)

    2011-07-15

    We present measurements of the excitation spectra from the 2p{sup 5}3p[5/2]{sub 3,2} levels in neon using two-step laser excitation and ionization in conjunction with an optogalvanic detection in dc and rf discharge cells. The 2p{sup 5}3p[5/2]{sub 3,2} intermediate levels have been approached via the collisionally populated 2p{sup 5}3s[3/2]{sub 2} metastable level. The Rydberg series 2p{sup 5}({sup 2}P{sub 3/2})nd[7/2]{sub 4} (12 {<=}n{<=} 44), 2p{sup 5}({sup 2}P{sub 3/2})ns[3/2]{sub 2} (13 {<=}n{<=} 35) and the parity forbidden transitions 2p{sup 5}({sup 2}P{sub 3/2})np[5/2]{sub 3} (13 {<=}n{<=} 19) have been observed from the 2p{sup 5}3p[5/2]{sub 3} level, whereas the 2p{sup 5}({sup 2}P{sub 3/2})nd[7/2]{sub 3} (12 {<=}n{<=} 44), 2p{sup 5}({sup 2}P{sub 3/2})ns[3/2]{sub 2} (13 {<=}n{<=} 35), and 2p{sup 5}({sup 2}P{sub 1/2})nd'[5/2]{sub 3} (9 {<=}n{<=} 12) Rydberg series have been observed from the 2p{sup 5}3p[5/2]{sub 2} level in accordance with the {Delta}J ={Delta}K = {+-} 1 selection rules. The photoionization cross sections from the 2p{sup 5}3p[5/2]{sub 3} intermediate level have been measured at eight ionizing laser wavelengths (399, 395, 390, 385, 380, 370, 364, and 355 nm) and that from the 2p{sup 5}3p[5/2]{sub 2} level at 401.8 nm. These measurements are in excellent agreement with the experimental values reported in the literature, while the experimental data lie much below the theoretically calculated photoionization cross sections curve.

  11. Correlation and relativistic effects in 2p photoelectron spectra of sodium atoms from the initial state 2{p}^{6}3p

    Science.gov (United States)

    Liu, Xiaobin; Shi, Yinglong; Xing, Yongzhong; Lu, Feiping; Chen, Zhanbin

    2017-02-01

    We investigate the 2p photoelectron spectra of sodium atoms with the initial state 2{p}63p at a photon energy of 54 eV. The analysis is performed based on the multi-configuration Dirac–Fock method. Special attention is given to the influences of correlation and relativistic effects on the spectra structures. To explore the nature and importance of such influences, calculations were performed based on detailed analyses of the thresholds, relative intensities and corresponding data calculated in the nonrelativistic limit.

  12. GROUND-STATE OF FERROMAGNETIC NICKEL AND MAGNETIC CIRCULAR-DICHROISM IN 2P AND 3P CORE X-RAY ABSORPTION SPECTROSCOPIES

    NARCIS (Netherlands)

    JO, T; YOSHIDA, A; SAWATZKY, GA

    The electronic state of ferromagnetic Ni and an interpretation of magnetic circular dichroism (MCD) in Ni 2p --> 3d and 3p --> 3d X-ray absorption (XAS) are discussed from a viewpoint of 3d configuration interaction. Inclusion of the 3d8 configuration and an orbital-magnetic-moment contribution of

  13. ELECTRONIC CORRELATIONS IN NI 2P AND 3P MAGNETIC-X-RAY DICHROISM AND X-RAY PHOTOEMISSION OF FERROMAGNETIC NICKEL

    NARCIS (Netherlands)

    VANDERLAAN, G; THOLE, BT

    1992-01-01

    The magnetic x-ray dichroism (MXD) in x-ray absorption together with the photoemission of the Ni 2p and 3p levels in ferromagnetic nickel have been analysed using an Anderson impurity model taking into account multiplet splitting. Good agreement with experimental results was obtained for a ground

  14. Yet1p-Yet3p interacts with Scs2p-Opi1p to regulate ER localization of the Opi1p repressor.

    Science.gov (United States)

    Wilson, Joshua D; Thompson, Sarah L; Barlowe, Charles

    2011-05-01

    Lipid sensing mechanisms at the endoplasmic reticulum (ER) coordinate an array of biosynthetic pathways. A major phospholipid regulatory circuit in yeast is controlled by Scs2p, an ER membrane protein that binds the transcriptional repressor protein Opi1p. Cells grown in the absence of inositol sequester Scs2p-Opi1p at the ER and derepress target genes including INO1. We recently reported that Yet1p and Yet3p, the yeast homologues of BAP29 and BAP31, are required for normal growth in the absence of inositol. Here we show that the Yet1p-Yet3p complex acts in derepression of INO1 through physical association with Scs2p-Opi1p. Yet complex binding to Scs2p-Opi1p was enhanced by inositol starvation, although the interaction between Scs2p and Opi1p was not influenced by YET1 or YET3 deletion. Interestingly, live-cell imaging analysis indicated that Opi1p does not efficiently relocalize to the ER during inositol starvation in yet3Δ cells. Together our data demonstrate that a physical association between the Yet complex and Scs2p-Opi1p is required for proper localization of the Opi1p repressor to ER membranes and subsequent INO1 derepression.

  15. Measurement of the 1s2s 1S0-1s2p 3P1 intercombination interval in helium-like silicon.

    Science.gov (United States)

    Redshaw, M; Myers, E G

    2002-01-14

    Using Doppler-tuned fast-beam laser spectroscopy the 1s2s 1S0-1s2p 3P1 intercombination interval in 28Si12+ has been measured to be 7230.5(2) cm(-1). The experiment made use of a single-frequency Nd:YAG (1.319 microm) laser and a high-finesse optical buildup cavity. The result provides a precision test of modern relativistic and QED atomic theory.

  16. Allelic variants of hexose transporter Hxt3p and hexokinases Hxk1p/Hxk2p in strains of Saccharomyces cerevisiae and interspecies hybrids.

    Science.gov (United States)

    Zuchowska, Magdalena; Jaenicke, Elmar; König, Helmut; Claus, Harald

    2015-11-01

    The transport of sugars across the plasma membrane is a critical step in the utilization of glucose and fructose by Saccharomyces cerevisiae during must fermentations. Variations in the molecular structure of hexose transporters and kinases may affect the ability of wine yeast strains to finish sugar fermentation, even under stressful wine conditions. In this context, we sequenced and compared genes encoding the hexose transporter Hxt3p and the kinases Hxk1p/Hxk2p of Saccharomyces strains and interspecies hybrids with different industrial usages and regional backgrounds. The Hxt3p primary structure varied in a small set of amino acids, which characterized robust yeast strains used for the production of sparkling wine or to restart stuck fermentations. In addition, interspecies hybrid strains, previously isolated at the end of spontaneous fermentations, revealed a common amino acid signature. The location and potential influence of the amino acids exchanges is discussed by means of a first modelled Hxt3p structure. In comparison, hexokinase genes were more conserved in different Saccharomyces strains and hybrids. Thus, molecular variants of the hexose carrier Hxt3p, but not of kinases, correlate with different fermentation performances of yeast.

  17. Evaluation of electron population terms for 4p, 3p, and (2p): how do HOMO and LUMO shrink or expand depending on nuclear charges?

    Science.gov (United States)

    Nakanishi, Waro; Hayashi, Satoko; Narahara, Kenji; Yamaki, Daisuke; Hada, Masahiko

    2008-01-01

    Electron population terms are evaluated for N=Se, S, and O. Calculations are performed on HOMO and LUMO constructed by pure atomic 4p(Se), 3p(S), and 2p(O) orbitals, employing the 6-311+G(3d) and/or 6-311(++)G(3df,3pd) basis sets at the HF, MP2, and DFT (B3 LYP) levels. Se(4+), Se(2+), Se(0), and Se(2-) with the O(h) symmetry are called G(A: Se) and HSe(+), H(2)Se, and HSe(-) with the C(infinityh) or C(2v) symmetry are named G(B: Se), here [G(A+B: Se) in all]. HOMO and LUMO in G(A+B: N) (N=Se, S, and O) satisfy the conditions of the calculations for . The (4p), (3p), and (2p) values correlate well with the corresponding MO energies (epsilon(N)) for all calculation levels employed. Plots of (HOMO) and (LUMO) versus Q(N) (N=Se, S, and O) at the HF and MP2 levels are analyzed as two correlations. However, the plots at the DFT level can be analyzed as single correlation. A regression curve is assumed for the analysis. Behaviors of clarify how valence orbitals shrink or expand depending on Q(N). The applicability of is examined to establish a new method that enables us to analyze chemical shifts with the charge effect separately from others. A utility program derived from the Gaussian 03 (NMRANAL-NH03G) is applied to evaluate and examine the applicability to the NMR analysis.

  18. Simultaneous measurements of photoionization cross-sections of lithium isotopes from 3p {sup 2}P{sub 1/2, 3/2}

    Energy Technology Data Exchange (ETDEWEB)

    Saleem, M; Hussain, Shahid; Rafiq, M; Baig, M A [Atomic and Molecular Physics Laboratory, Department of Physics, Quaid-i-Azam University, 45320 Islamabad (Pakistan)

    2006-12-28

    We present the first measurements of the photoionization cross-section of the 3p {sup 2}P{sub 1/2, 3/2} excited states for both the lithium isotopes Li{sup 6} and Li{sup 7} in the region close to the Li{sup +} 1s{sup 2 1}S threshold (from 0 to 3 eV) using the two-step photoionization and the saturation technique. Narrow linewidth dye lasers have been used in combination with a locally developed atomic beam-TOF mass spectrometer to measure the photoion signals as a function of energy density of the ionizing laser. It is observed that the cross-section is maximum near the ionization threshold and decreases monotonically as the excess photon energy of the ionizing laser is increased. The deduced number densities of the isotopes are Li{sup 6} (1.2 x 10{sup 8} atoms cm{sup -3}) and Li{sup 7} (1.2 x 10{sup 9} atoms cm{sup -3}), which are found to be close to their natural abundance ratio within the experimental error. Our experimental results of the measurement of the cross-section are also in good agreement with earlier theoretical work.

  19. RECENT ADVANCES IN THE 5Q- SYNDROME

    Directory of Open Access Journals (Sweden)

    Andrea Pellagatti

    2015-05-01

    Full Text Available The 5q- syndrome is the most distinct of the myelodysplastic syndromes (MDS and patients with this disorder have a deletion of chromosome 5q [del(5q] as the sole karyotypic abnormality. Several genes mapping to the commonly deleted region of the 5q- syndrome have been implicated in disease pathogenesis in recent years. Haploinsufficiency of the ribosomal gene RPS14 has been shown to cause the erythroid defect in the 5q- syndrome. Loss of the microRNA genes miR-145 and miR-146a has been associated with the thrombocytosis observed in 5q- syndrome patients. Haploinsufficiency of CSNK1A1 leads to hematopoietic stem cell expansion in mice and may play a role in the initial clonal expansion in patients with 5q- syndrome. Moreover, a subset of patients harbor mutation of the remaining CSNK1A1 allele. Mouse models of the 5q- syndrome, which recapitulate the key features of the human disease, indicate that a p53-dependent mechanism underlies the pathophysiology of this disorder. Importantly, activation of p53 has been demonstrated in the human 5q- syndrome. Recurrent TP53 mutations have been associated with an increased risk of disease evolution and with decreased response to the drug lenalidomide in del(5q MDS patients. Potential new therapeutic agents for del(5q MDS include the translation enhancer L-leucine.

  20. Synthesis and characterization of fac-[M(CO)3(P)(OO)] and cis-trans-[M(CO)2(P)2(OO)] complexes (M = Re, (99m)Tc) with acetylacetone and curcumin as OO donor bidentate ligands.

    Science.gov (United States)

    Triantis, Charalampos; Tsotakos, Theodoros; Tsoukalas, Charalampos; Sagnou, Marina; Raptopoulou, Catherine; Terzis, Aris; Psycharis, Vassilis; Pelecanou, Maria; Pirmettis, Ioannis; Papadopoulos, Minas

    2013-11-18

    The synthesis and characterization of neutral mixed ligand complexes fac-[M(CO)3(P)(OO)] and cis-trans-[M(CO)2(P)2(OO)] (M = Re, (99m)Tc), with deprotonated acetylacetone or curcumin as the OO donor bidentate ligands and a phosphine (triphenylphosphine or methyldiphenylphosphine) as the monodentate P ligand, is described. The complexes were synthesized through the corresponding fac-[M(CO)3(H2O)(OO)] (M = Re, (99m)Tc) intermediate aqua complex. In the presence of phosphine, replacement of the H2O molecule of the intermediate complex at room temperature generates the neutral tricarbonyl monophosphine fac-[Re(CO)3(P)(OO)] complex, while under reflux conditions further replacement of the trans to the phosphine carbonyl generates the new stable dicarbonyl bisphosphine complex cis-trans-[Re(CO)2(P)2(OO)]. The Re complexes were fully characterized by elemental analysis, spectroscopic methods, and X-ray crystallography showing a distorted octahedral geometry around Re. Both the monophosphine and the bisphosphine complexes of curcumin show selective binding to β-amyloid plaques of Alzheimer's disease. At the (99m)Tc tracer level, the same type of complexes, fac-[(99m)Tc(CO)3(P)(OO)] and cis-trans-[(99m)Tc(CO)2(P)2(OO)], are formed introducing new donor combinations for (99m)Tc(I). Overall, β-diketonate and phosphine constitute a versatile ligand combination for Re(I) and (99m)Tc(I), and the successful employment of the multipotent curcumin as β-diketone provides a solid example of the pharmacological potential of this system.

  1. Genetics Home Reference: 5q minus syndrome

    Science.gov (United States)

    ... for ribosomal protein genes causes selective activation of p53 in human erythroid progenitor cells. Blood. 2011 Mar ... U, List A, MacBeth KJ. Lenalidomide as a disease-modifying agent in patients with del(5q) myelodysplastic ...

  2. Photoionization of the Be-like O{sup 4} {sup +} ion: total and partial cross sections for the ground 2s{sup 2} {sup 1}S and excited 2s2p {sup 1,} {sup 3}P states

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dae-Soung [e-Business Department. Kyonggi Institute of Technology, Siheung, Jungwang-Dong 2121-3, Kyonggi-Do 429-792 (Korea, Republic of); Manson, Steven T, E-mail: dskim@kinst.ac.k [Department of Physics and Astronomy, Georgia State University, Atlanta, GA 30303 (United States)

    2010-08-14

    Photoionization cross sections of the Be-like O{sup 4} {sup +} ion in the photon energy region from the first threshold up to the O{sup 5} {sup +} 3d threshold have been calculated using a non-iterative variational R-matrix method combined with multichannel quantum-defect theory for the ground 2s{sup 2} {sup 1}S and excited 2s2p {sup 1,} {sup 3}P states. The partial cross sections are presented and the autoionizing resonance structures arising from the ground and excited states are identified and characterized. Our calculational results, which show excellent agreement between length and velocity gauges, are compared with the available experiment and previous calculations, and good agreement is found.

  3. Characteristics of photoionization in the XUV domain for the excited 1s{sup 2}2s2p{sup 1,3}P{sup o} states of the Be-like C{sup 2+} ion

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dae-Soung [e-Business Department, Kyonggi Institute of Technology, Siheung, Jungwang-Dong 2121-3, Kyonggi-Do 429-450 (Korea, Republic of); Kim, Young Soon [Department of Physics, Myongji University, Yongin 449-728 (Korea, Republic of)

    2007-10-14

    Resonance structures and their characteristics in the photoionization of the first excited p-states of Be-like C{sup 2+} ion which interact with the even-parity continua have been studied up to the C{sup 3+} 3d threshold limit. Using the variational R-matrix method, calculations have been performed in the initial 20 and final 22 state close-coupling approximations in the photon wavelength range 15-35 nm. The detailed photoionization processes of included multichannels are displayed in the partial photoionization cross sections, which contain a number of autoionizing Rydberg series of resonances converging to the C{sup 3+} 2p, 3s, 3p and 3d thresholds. In particular, the lower members of the Rydberg series showing irregular patterns of resonances, which lie above the C{sup 3+} 2p threshold, are identified and resonance positions E{sub r}, effective quantum numbers n*, and widths {gamma} are presented. Excellent agreement between length and velocity gauge results is found, along with overall good agreement with previous work, except for the discrepancies with the threshold behaviour of the OP (opacity project) result.

  4. Alignment of Ar{sup +} [{sup 3}P]4p{sup 2}P{sup 0}{sub 3/2} satellite state from the polarization analysis of fluorescent radiation after photoionization

    Energy Technology Data Exchange (ETDEWEB)

    Yenen, O.; McLaughlin, K.W.; Jaecks, D.H. [Univ. of Nebraska, Lincoln, NE (United States)] [and others

    1997-04-01

    The measurement of the polarization of radiation from satellite states of Ar{sup +} formed after the photoionization of Ar provides detailed information about the nature of doubly excited states, magnetic sublevel cross sections and partial wave ratios of the photo-ejected electrons. Since the formation of these satellite states is a weak process, it is necessary to use a high flux beam of incoming photons. In addition, in order to resolve the many narrow doubly excited Ar resonances, the incoming photons must have a high resolution. The characteristics of the beam line 9.0.1 of the Advanced Light Source fulfill these requirements. The authors determined the polarization of 4765 {Angstrom} fluorescence from the Ar{sup +} [{sup 3}P] 4p {sup 2}P{sub 3/2}{sup 0} satellite state formed after photoionization of Ar by photons from the 9.0.1 beam line of ALS in the 35.620-38.261 eV energy range using a resolution of approximately 12,700. This is accomplished by measuring the intensities of the fluorescent light polarized parallel (I{parallel}) and perpendicular (I{perpendicular}) to the polarization axis of the incident synchrotron radiation using a Sterling Optics 105MB polarizing filter. The optical system placed at 90{degrees} with respect to the polarization axis of the incident light had a narrow band interference filter ({delta}{lambda}=0.3 nm) to isolate the fluorescent radiation.

  5. Relativistic evaluation of the two-photon decay of the metastable 1 s22 s 2 p 3P0 state in berylliumlike ions with an effective-potential model

    Science.gov (United States)

    Amaro, Pedro; Fratini, Filippo; Safari, Laleh; Machado, Jorge; Guerra, Mauro; Indelicato, Paul; Santos, José Paulo

    2016-03-01

    The two-photon 1 s22 s 2 p 3P0→1 s2s21S0 transition in berylliumlike ions is investigated theoretically within a fully relativistic framework and a second-order perturbation theory. We focus our analysis on how electron correlation, as well as the negative-energy spectrum, can affect the forbidden E 1 M 1 decay rate. For this purpose, we include the electronic correlation via an effective local potential and within a single-configuration-state model. Due to its experimental interest, evaluations of decay rates are performed for berylliumlike xenon and uranium. We find that the negative-energy contribution can be neglected at the present level of accuracy in the evaluation of the decay rate. On the other hand, if contributions of electronic correlation are not carefully taken into account, it may change the lifetime of the metastable state by up to 20%. By performing a fully relativistic j j -coupling calculation, we find a decrease of the decay rate by two orders of magnitude compared to nonrelativistic L S -coupling calculations, for the selected heavy ions.

  6. Main: 1B5Q [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available 1B5Q トウモロコシ Corn Zea mays L. Polyamine Oxidase Precursor Name=Pao; Zea Mays Molecul...EESRRIEQQSDEQTKAEIMQVLRKMFPGKDVPDATDILVPRWWSDRFYKGTFSNWPVGVNRYEYDQLRAPVGRVYFTGEHTSEHYNGYVHGAYLSGIDSAEILINCAQKKMCKYHVQGKYD corn_1B5Q.jpg ...

  7. Calculation of the total electron excitation cross section in the Born approximation using Slater wave functions for the Li (2s yields 2p), Li (2s yields 3p), Na (3s yields 4p), Mg (3p yields 4s), Ca (4s yields 4p) and K (4s yields 4p) excitations. M.S. Thesis

    Science.gov (United States)

    Simsic, P. L.

    1974-01-01

    Excitation of neutral atoms by inelastic scattering of incident electrons in gaseous nebulae were investigated using Slater Wave functions to describe the initial and final states of the atom. Total cross sections using the Born Approximation are calculated for: Li(2s yields 2p), Na(3s yields 4p), k(4s yields 4p). The intensity of emitted radiation from gaseous nebulae is also calculated, and Maxwell distribution is employed to average the kinetic energy of electrons.

  8. APPARENT SMA-I UNLINKED TO 5Q

    NARCIS (Netherlands)

    COBBEN, JM; SCHEFFER, H; DEVISSER, M; BEGEER, JH; MOLENAAR, WM; VANDERSTEEGE, G; BUYS, CHCM; VANOMMEN, GJ; TENKATE, LP

    A proband with a clinical picture indistinguishable from SMA type I is described. The parents are second cousins. On DNA analysis it appeared that the proband and his healthy 2 year old sib had inherited the same haplotypes for DNA markers flanking the SMA locus on 5q. This supports non-linkage of

  9. A locus regulating bronchial hyperresponsiveness maps to chromosome 5q

    Energy Technology Data Exchange (ETDEWEB)

    Levitt, R.C.; Meyers, D.A. [Johns Hopkins Medical Institutions, Baltimore, MD (United States); Bleecker, E.R. [Univ. of Maryland, Baltimore, MD (United States)] [and others

    1994-09-01

    Bronchial hyperresponsiveness (BHR) is one of the hallmarks of asthma. BHR correlates well with asthmatic symptoms and the response to treatment. Moreover, BHR appears to be closely related to airways inflammation. Numerous studies have demonstrated a familial aggregation; however, this phenotype is not likely inherited as a simple Mendelian trait. BHR is also closely associated with total serum IgE levels, as are allergy and asthma. We studied 92 families from Northern Holland ascertained through a parent with asthma who were originally studied between 1962-1970. Since there are a number of candidate genes on chromosome 5q potentially important in producing BHR, families were genotyped for markers in this region. These genes regulate IgE production and the cellular elements that are likely involved in inflammation associated with BHR, allergy and asthma. They include IL-4, IL-3, IL-5, IL-9, IL-12, IL-13 and GM-CSF. Linkage of BHR with markers on 5q was tested using a model free sib-pair method. The data suggest a locus for BHR maps near the cytokine gene cluster on 5q. This region appears critical in producing susceptibility to BHR and possibly to asthma.

  10. Síndrome 5q-: um relato de caso

    Directory of Open Access Journals (Sweden)

    Marcelo Gil Cliquet

    2015-10-01

    Full Text Available As síndromes mielodisplásicas (SMD se caracterizam por hematopoiese ineficaz, citopenias, distúrbios qualitativos de uma ou mais linhagens de células do sangue periférico, anormalidades cromossômicas e uma predileção variável de evolução para leucemias mielóides agudas. As anormalidades mais freqüentemente observadas incluem a deleção do braço longo do cromossomo 5 (5q-. O tratamento com novas drogas vem mudando as perspectivas de tratamento. A lenalidomida é um fármaco que fica reservada para o tratamento das síndromes mielodisplásicas 5q- e mieloma múltiplo. Objetivo: Relatar o uso do fármaco lenalidomida por um paciente portador de SMD com a síndrome 5q-, avaliando o impacto na necessidade transfusional. Relato de caso: Paciente de 64 anos, sexo masculino, encaminhado por apresentar anemia (8,2g/dl macrocítica (VCM=109fl. Série branca e plaquetária normais. Após investigação para hemólise, carência de B12, ácido fólico e Ferro sem anormalidades, foi solicitada avaliação de medula óssea que revelou hipercelularidade (relação G/E=4/1, aumento de mieloblastos e prómielocitos, e cariótipo mostrando 46XY del(5(q15q33{6} e 46XY{14}. Prescrito Lenalidomida (10mg VO/dia em ciclos de 21 dias com intervalo de uma semana. Desde a primeira consulta até o início do tratamento, o paciente recebeu 16 unidades de concentrados de hemácias. Após dois ciclos, observou-se elevação da hemoglobina, sem transfusões, chegando a 12,3g/dl. Apresentou, no entanto, redução de neutrófilos (970/mm³ e de plaquetas (107.000/mm3. Conclusão: O uso do fármaco pode melhorar a qualidade de vida e a sobrevida dos pacientes portadores da síndrome 5q-, tendo em vista a redução de transfusões de sangue, evitando, assim, a sobrecarga de ferro e ainda pode diminuir a taxa de transformação em LMA.

  11. On the kinetic and thermodynamic fragility of the Pt{sub 60}Cu{sub 16}Co{sub 2}P{sub 22} and Pt{sub 57.3}Cu{sub 14.6}Ni{sub 5.3}P{sub 22.8} bulk metallic glasses

    Energy Technology Data Exchange (ETDEWEB)

    Gallino, Isabella, E-mail: i.gallino@mx.uni-saarland.de [Saarland University, Department of Materials Science and Engineering, Campus C6.3, 66123 Saarbruecken (Germany); Gross, Oliver [Saarland University, Department of Materials Science and Engineering, Campus C6.3, 66123 Saarbruecken (Germany); Dalla Fontana, Giulia [Department of Chemistry IFM and NIS, University of Torino, V. Giuria 7, 10125 Torino (Italy); Evenson, Zach; Busch, Ralf [Saarland University, Department of Materials Science and Engineering, Campus C6.3, 66123 Saarbruecken (Germany)

    2014-12-05

    Highlights: • The studied Pt–Cu–(Ni,Co)–P glasses are more fragile than Zr-based alloys. • They show large increases in the C{sub p} at T{sub g} and small barriers for cooperative rearrangements of atoms. • They have fragility parameters among the lowest reported for BMG systems (D{sup *} = 10–12). • They crystallize into a state that melts with distinctly high entropy of fusion. • The microscopic origin of their fragility seems different than that for Zr- and Pd-based BMGs. - Abstract: The investigations in this study focus on bulk metallic glass (BMG) alloy families based on noble metals like Pt, which are more kinetically fragile than Zr-based BMG systems. Thermophysical properties have been investigated by calorimetry and thermal mechanical analyses for the determination of the specific heat capacity and viscosity, respectively. For the Pt{sub 60}Cu{sub 16}Co{sub 2}P{sub 22} and Pt{sub 57.3}Cu{sub 14.6}Ni{sub 5.3}P{sub 22.8} BMG compositions consistent Vogel–Fulcher–Tammann (VFT) fits of the viscosity measurements are established, and the temperature dependence of the configurational entropy is calculated from thermodynamic data. Fits to the Adam–Gibbs equation are performed using this configurational entropy change. Their fragile nature is compared to that of Zr-based alloys in terms of structural considerations.

  12. SPIN-FORBIDDEN TRANSITIONS FOR BE-LIKE IONS (Z=10-103)%高离化类铍离子2s2 1S0-2s2p3P1(Z=10-103)自旋禁戒光谱跃迁

    Institute of Scientific and Technical Information of China (English)

    易有根; 汪蓉; 李向东; 王红艳; 朱正和

    2000-01-01

    利用全相对论性多组态Dirac-Fock平均能级(MCDF-AL)方法系统地计算了高离化类铍离子自旋禁戒2s2 1S0-2s2p3P1(Z=10-103)跃迁的能级间隔和跃迁概率,计算中考虑了重要的核的有限体积效应,Breit修正和QED修正,所得结果和最近的实验数据及理论计算值进行了比较,结果表明:高原子序数的高荷电离子的自旋禁戒跃迁的跃迁概率和中性原子的电偶极E1的相当,在ICF和MCF高温激光等离子体中,自旋禁戒跃迁概率过程不容被忽视.

  13. Síndrome 5q-: um relato de caso

    OpenAIRE

    Marcelo Gil Cliquet; Fábio Moreira Campos; Gabriel Murad Takao; Ricardo Hiroshi Murashita Fujiki; Thays Brunelli Pugliesi; Guilherme Cliquet

    2015-01-01

    As síndromes mielodisplásicas (SMD) se caracterizam por hematopoiese ineficaz, citopenias, distúrbios qualitativos de uma ou mais linhagens de células do sangue periférico, anormalidades cromossômicas e uma predileção variável de evolução para leucemias mielóides agudas. As anormalidades mais freqüentemente observadas incluem a deleção do braço longo do cromossomo 5 (5q-). O tratamento com novas drogas vem mudando as perspectivas de tratamento. A lenalidomida é um fármaco que fica reservada p...

  14. PP2A: The Achilles Heal in MDS with 5q Deletion

    Directory of Open Access Journals (Sweden)

    David eSallman

    2014-09-01

    Full Text Available Myelodysplastic syndromes (MDS represent a hematologically diverse group of myeloid neoplasms, however, one subtype characterized by an isolated deletion of chromosome 5q (del(5q is pathologically and clinically distinct. Patients with del(5q MDS share biological features that account for the profound hypoplastic anemia and unique sensitivity to treatment with lenalidomide. Ineffective erythropoiesis in del(5q MDS arises from allelic deletion of the ribosomal processing S-14 (RPS14 gene, which leads to MDM2 sequestration with consequent p53 activation and erythroid cell death. Since its approval in 2005, lenalidomide has changed the natural course of the disease. Patients who achieve transfusion independence and/or a cytogenetic response with lenalidomide have a decreased risk of progression to AML and an improved overall survival compared to non-responders. Elucidation of the mechanisms of action of lenalidomide in del(5q MDS has advanced therapeutic strategies for this disease. The selective cytotoxicity of lenalidomide in del(5q clones derives from inhibition of a haplodeficient phosphatase whose catalytic domain is encoded within the common deleted region on chromosome 5q, i.e., protein phosphatase 2A (PP2Acα. PP2A is a highly conserved, dual specificity phosphatase that plays an essential role in regulation of the G2/M checkpoint. Inhibition of PP2Acα results in cell cycle arrest and apoptosis in del(5q cells. Targeted knockdown of PP2Acα using siRNA is sufficient to sensitize non-del(5q clones to lenalidomide. Through its inhibitory effect on PP2A, lenalidomide stabilizes MDM2 to restore p53 degradation in erythroid precursors, with subsequent arrest in G2/M. Unfortunately, the majority of patients with del(5q MDS develop resistance to lenalidomide over time associated with PP2Acα overexpression. Targeted inhibition of PP2A with a more potent inhibitor has emerged as an attractive therapeutic approach for patients with del(5q MDS.

  15. An unusual combination of trisomy 21 and partial trisomy 5q.

    Science.gov (United States)

    Kim, C. J.; Chi, J. G.; Lee, K. H.; Lee, C. K.; Yoo, M. S.; Paik, Y. K.

    1992-01-01

    The authors describe a male newborn with multiple congenital anomalies; craniofacial dysmorphism, bilateral cleft palate and lip, ambiguous external genitalia with absence of phallus, ventricular septal defect, agenesis of olfactory bulbs, and presence of small round cells simulating migration defect in the cerebellar white matter. Cytogenetic study demonstrated a chromosomal constitution of 47,XY, +21, +5q. Its pathological significance compared with Down's syndrome and hitherto reported partial trisomy 5q is discussed. PMID:1299243

  16. Saperi P2P

    Directory of Open Access Journals (Sweden)

    Salvatore Iaconesi

    2009-10-01

    Full Text Available Il paper presenta l'architettura filosofica e logica di un progetto ongoing per la creazione di un'infrastruttura peer to peer per la diffusione dei saperi. Tale infrastruttura p2p vuole essere la base per costruire un framework aperto e orizzontale, che ospiti pratiche innovative di creazione, condivisione e disseminazione di informazioni e conoscenza.

  17. Partial trisomy 5q resulting from chromosome 7 insertion: An expansion of the phenotype

    Energy Technology Data Exchange (ETDEWEB)

    Fries, M.H.; Reilly, P.A.; Williams, T.C. [Keesler Medical Center, MS (United States)] [and others

    1994-09-01

    Partial trisomy 5q has been categorized into three separate phenotypes; however, a distinctive phenotype has not been described for duplications spanning 5q23-q35. We report a case of partial trisomy 5q for this region as a result of a ins(7,5)(q31.3;q23.2q35.1)mat. The liveborn male infant was delivered by emergency cesarean section at 37 weeks after a pregnancy notable for oligohydramnios, with birth weight 1792 g (<3%). Postnatal course was marked by psychomotor delay, failure to thrive, and biopsy demonstrated neonatal giant cell hepatitis with a paucity of intrahepatic bile ducts. His appearance was remarkable for lack of subcutaneous fat, midline displaced hair whorl, bitemporal narrowing with frontal bossing, wide anterior fontanel, widow`s peak, protuberant eyes with periorbital and lid edema, short flat nasal bridge with broad flattened nasal tip, long smooth philtrum, wide mouth with thin lips, wide gingival ridges, micrognathia, posteriorly rotated low-set ears, hepatomegaly, flexion contractions of elbows, and generalized hypertonicity. Urine organic acids, oligosaccharide/mucopolysaccharide screen, and plasma amino acids were negative. GTG-banding on prometaphase chromosomes showed an unbalanced translocation involving chr. 7. This was identified as an insertion of chr. 5 (q23.2q35.1) into distal 7q after FISH using chr. 5 and chr. 7 painting probes. The infant`s mother carries the balanced insertional rearrangement: 46,XX,dir ins(7,5)(q31.3;q23.2q35.1). This phenotype overlaps that of previously described duplications with the addition of giant cell hepatitis, coarsened facial features, gingival thickening, and flexion contractures, suggestive of a yet undiagnosed storage disorder.

  18. Refractory primary immune thrombocytopenia with subsequent del(5q) MDS: complete remission of both after lenalidomide.

    Science.gov (United States)

    Mortensen, Thomas Bech; Frederiksen, Henrik; Marcher, Claus Werenberg; Preiss, Birgitte

    2017-01-04

    A patient with refractory primary immune thrombocytopenia (ITP) characterised by severe skin and mucosal bleedings was treated with several ITP-directed therapies including cyclophosphamide. He later developed therapy-related del(5q) myelodysplastic syndrome with no dysplastic morphological features in bone marrow. He remained severely thrombocytopenic, which suggests ongoing immune mediated platelet destruction. After two 3 week cycles of low-dose lenalidomide, complete cytogenetic remission and complete normalisation of platelet count were observed. This suggests that lenalidomide may be a viable treatment option for ITP in the presence of del(5q) not responding to standard treatments.

  19. Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDS.

    Science.gov (United States)

    Krönke, Jan; Fink, Emma C; Hollenbach, Paul W; MacBeth, Kyle J; Hurst, Slater N; Udeshi, Namrata D; Chamberlain, Philip P; Mani, D R; Man, Hon Wah; Gandhi, Anita K; Svinkina, Tanya; Schneider, Rebekka K; McConkey, Marie; Järås, Marcus; Griffiths, Elizabeth; Wetzler, Meir; Bullinger, Lars; Cathers, Brian E; Carr, Steven A; Chopra, Rajesh; Ebert, Benjamin L

    2015-07-09

    Lenalidomide is a highly effective treatment for myelodysplastic syndrome (MDS) with deletion of chromosome 5q (del(5q)). Here, we demonstrate that lenalidomide induces the ubiquitination of casein kinase 1A1 (CK1α) by the E3 ubiquitin ligase CUL4-RBX1-DDB1-CRBN (known as CRL4(CRBN)), resulting in CK1α degradation. CK1α is encoded by a gene within the common deleted region for del(5q) MDS and haploinsufficient expression sensitizes cells to lenalidomide therapy, providing a mechanistic basis for the therapeutic window of lenalidomide in del(5q) MDS. We found that mouse cells are resistant to lenalidomide but that changing a single amino acid in mouse Crbn to the corresponding human residue enables lenalidomide-dependent degradation of CK1α. We further demonstrate that minor side chain modifications in thalidomide and a novel analogue, CC-122, can modulate the spectrum of substrates targeted by CRL4(CRBN). These findings have implications for the clinical activity of lenalidomide and related compounds, and demonstrate the therapeutic potential of novel modulators of E3 ubiquitin ligases.

  20. Duplication of 5q21 in a mildly retarded male and his non-retarded mother

    Energy Technology Data Exchange (ETDEWEB)

    Stallard, R.; Zurcher, V.; Schwartz, S. [Case Western Reserve Univ., Cleveland, OH (United States)

    1994-09-01

    Euchromatic autosomal additions to chromosomal complements are typically associated with global effects including mental retardation (MR) and dysmorphism. We report a familial duplication that does not appear to cause consistent, significant effects. A hyperactive male with mild MR was referred for fra(X) testing at 8 yrs. His karyotype was fra(X) negative and normal except for an addition in one 5q. The abnormal 5 was also in the maternal karyotype, but all other parental chromosomes were normal. The addition (=8.5% the length of a 5) was interpreted as a duplication of band 5q21. FISH with Coatasome 5 (Oncor) showed the addition was from 5. The proband`s karyotype was designated 46,XY,dup(5)(q15q22.1)mat; his mother`s, 46,XX,dup(5)(q15q22.1). Single copy probes are being used to test the cytogenetic interpretation. At 39 yrs, the non-retarded, somewhat inattentive mother, who has a high school diploma and subsequent secretarial courses, cares for the proband and his chromosomally normal, but learning disabled sister at home. The family situation is chaotic with reported paternal psychiatric illness and abuse of the proband and his sister. The mother`s father is dead, but her four younger siblings and mother are reportedly normal. Their chromosomes have not been available. The proband was born at 40 weeks following an uneventful pregnancy, with length and weight at the 5-10th centiles. He walked and talked at about one year. At 9 yrs, his ht/wt ratio was 10th centile. Foot length as <3rd centile; soft masses were present on the anterior ankles. He was otherwise physically normal. His estimated I.Q. was 75 and he was severely hyperactive despite Ritalin. This is the first report of a familial duplication in 5q; no identical, isolated case is known. Although additional family members need evaluation, the presence of the dup(5q) in the non-retarded mother suggests that it may not be associated with the proband`s MR.

  1. Interstitial deletion of 5q33.3q35.1 in a boy with severe mental retardation

    OpenAIRE

    Lee, Jin Hwan; Kim, Hyo Jeong; Yoon, Jung Min; Cheon, Eun Jung; Lim, Jae Woo; Ko, Kyong Og; Lee, Gyung Min

    2016-01-01

    Constitutional interstitial deletions of the long arm of chromosome 5 (5q) are quite rare, and the corresponding phenotype is not yet clearly delineated. Severe mental retardation has been described in most patients who present 5q deletions. Specifically, the interstitial deletion of chromosome 5q33.3q35.1, an extremely rare chromosomal aberration, is characterized by mental retardation, developmental delay, and facial dysmorphism. Although the severity of mental retardation varies across cas...

  2. [Lenalidomide treatment in myelodysplastic syndrome with 5q deletion--Czech MDS group experience].

    Science.gov (United States)

    Jonášová, Anna; Červinek, Libor; Bělohlávková, Petra; Čermák, Jaroslav; Beličková, Monika; Rohoň, Petr; Černá, Olga; Hochová, Ivana; Šišková, Magda; Kačmářová, Karla; Janoušová, Eva

    2015-12-01

    Myelodysplastic syndrome (MDS) is a common hematological disease in patients over sixty. Despite intensive research, the therapy of this heterogeneous blood disease is complicated. In recent years, two new therapeutic approaches have been proposed: immunomodulation and demethylation therapy. Immunomodulation therapy with lenalidomide represents a meaningful advance in the treatment of anemic patients, specifically those with 5q- aberrations. As much as 60-70% of patients respond and achieve transfusion independence. We present the initial lenalidomide experience of the Czech MDS group. We analyze Czech MDS register data of 34 (31 female; 3 male; median age 69 years) chronically transfused low risk MDS patients with 5q- aberration treated by lenalidomide. Twenty-seven (79.4%) patients were diagnosed with 5q- syndrome, 5 patients with refractory anemia with multilineage dysplasia, 1 patient with refractory anemia with excess of blasts 1, and 1 patient with myelodysplastic/myeloproliferative unclassified. Response, as represented by achieving complete transfusion independence, was achieved in 91% of patients. A true 5q- syndrome diagnosis in most our patients may be responsible for such a high response rate. Complete cytogenetic response was reached in 15% of patients and partial cytogenetic response in 67%, within a median time of 12 months. TP53 mutation was detected in 15% (3 from 18 tested) and 2 of these patients progressed to higher grade MDS. The majority of patients tolerated lenalidomide very well. Based on this albeit small study, we present our findings of high lenalidomide efficacy as well as the basic principles and problems of lenalidomide therapy.

  3. Radiation hybrid mapping of a cytokine gene cluster located in the proximal region of 5q

    Energy Technology Data Exchange (ETDEWEB)

    Segal, A.L.; McPherson, J.D.; Wasmuth, J.J. [Univ. of California, Irvine (United States)

    1994-09-01

    The long (q) arm of chromosome 5 has been shown to contain a large number of genes encoding growth factors, growth factor receptors, hormone receptors and neurotransmitter receptors. IL-3, IL-4, IL-5, IL-9, IL-13, GM-CSF and IRF-1 are located in the 5q22-31.1 interval, while three GABA receptors map to 5q33-34. A number of receptors, including the prolactin and growth hormone receptors, the IL-7 receptor and the leukemia inhibitory factor receptor, map to proximal 5p. Genes encoding three of the complement components, C6, C7 and C9, are also located in the same region. YAC data indicates that C6 and C7 lie within 170 kb of each other. We have used a panel of 180 Chinese hamster-human radiation hybrids possessing fragments of human chromosome 5 to construct a physical map of this region of 5q. Two-point and multi-point analyses were done on the data and significant LOD scores (from 3 to 30) were observed. LIFR, PRLR, GHR, IL-7R, C6, C7, C9, TARS, and a number of CEPH-Genethon dinucleotide repeat markers were ordered and mapped. Yeast artificial chromosomes and cosmids have been isolated and inter-Alu PCR products from them are being used to construct a contig and to improve the physical map. The long term goal of this work is to identify and characterize new genes in the region.

  4. A locus regulating total serum IgE maps to chromosome 5q

    Energy Technology Data Exchange (ETDEWEB)

    Amelung, P.J.; Panhuysen, C.I.M.; Postma, D.S. [Univ. of Maryland, Baltimore, MD (United States)]|[Beatrixoord Hospital (Netherlands)

    1994-09-01

    Familial aggregation of allergy has been demonstrated in numerous past studies. However, allergy is a complex disorder which is not inherited as a simple Mendelian trait. Total serum IgE levels correlate with the clinical expression of allergy and asthma and can be utilized as a quantitative measure of the allergic phenotype. We studied 92 families from Northern Holland ascertained through a parent with asthma who were originally studied between 1962-1970. Since there is a large number of candidate genes on chromosome 5q, families were genotyped for markers in this region. These genes either directly or indirectly regulate IgE production and the activation and proliferation of cellular elements that are involved in inflammation associated with allergy and asthma. They include IL-4, IL-3, IL-5, IL-9, IL-12, IL-13 and GM-CSF. Segregation analyses revealed recessive inheritance of `high` levels with a mean for the `low` phenotype of 1.51 (32 IU) and 2.52 (331 IU) for the `high` phenotype. Linkage of log IgE with markers on 5q was tested using the sib-pair and the LOD score methods with the genetic model obtained from the segregation analyses. These results provide evidence for a locus controlling IgE levels near the cytokine gene cluster on 5q. This region appears critical in susceptibility to allergy and asthma.

  5. Lenalidomide for myelodysplastic syndromes with del(5q): how long should it last?

    Science.gov (United States)

    Vozella, Federico; Latagliata, Roberto; Carmosino, Ida; Volpicelli, Paola; Montagna, Chiara; Romano, Angela; Roberto, Amanda; Finsinger, Paola; Mancini, Marco; Breccia, Massimo; Oliva, Esther; Oliva, Esther

    2015-03-01

    Lenalidomide induces in patients with myelodysplastic syndrome (MDS) and del(5q) erythroid and cytogenetic response rates as high as 75% and 50%, respectively. It is still unclear, however, how long lenalidomide treatment should be continued and whether or not the drug could be interrupted. To assess the feasibility of lenalidomide discontinuation, we revised a cohort of 16 low-risk MDS patients with del(5q) treated at our institute in a phase II multicentric Italian study. Among the 12 responding patients, four discontinued lenalidomide while in complete response. All four patients needed during treatment a permanent lenalidomide reduction from 10 to 5 mg/day because of haematological toxicity (three patients) or grade 3 muscular and bone pain (one patient). At lenalidomide discontinuation after 16, 20, 27 and 20 months from the start, respectively, all four patients were in complete hematologic response and three forth in complete cytogenetic response. Three patients are still in response after 36, 30 and 20 months from lenalidomide discontinuation, respectively: The remaining patient relapsed after 20 months, and she is now receiving a new course of lenalidomide. In conclusion, long-lasting remissions are achievable in MDS patients with del(5q) in complete response after lenalidomide discontinuation.

  6. Distal 5q trisomy resulting from an X;5 translocation detected by chromosome painting.

    Science.gov (United States)

    Abuelo, D N; Ahsanuddin, A N; Mark, H F

    2000-10-23

    We describe the case of a 13-year-old girl with an apparently de novo unbalanced translocation resulting in the presence of additional chromosomal material on the short arm of one X chromosome, which was detected by conventional G-banding studies. Fluorescence in situ hybridization (FISH) using the Chromoprobe Multiprobe-M protocol confirmed that the additional chromosomal material originated from chromosome 5. The karyotype of this patient is now established to be 46,X,der(X) t(X;5)(p22.3;q33), with a deletion of Xp22.3-pter and partial trisomy of 5q33-qter. The distal 5q trisomy genotype has been associated with clinical signs that include growth and mental retardation, eczema, craniofacial anomalies, and malformations of heart, lungs, abdomen, limbs, and genitalia. Our patient also has short stature, a prominent nasal bridge, a flat philtrum, a thin upper lip, dental caries, and limb and cardiac malformations, but she appears to be mildly affected compared with previously reported cases. This is the first case of distal 5q trisomy arising from a translocation with the X chromosome. Replication studies on this patient show that the derivative t(X;5) chromosome is late replicating in almost all cells examined, which indicates that this chromosome is preferentially inactivated. However, the translocated segment of chromosome 5 appears to be early replicating, which implies that the trisomic 5q segment is transcriptionally active. We cannot determine from these studies whether all or only some genes in this segment are expressed, but this patient's relatively mild clinical signs suggest that the critical region(s) that contribute to the distal 5q trisomy phenotype are at least partly suppressed. A review of other patients with X-chromosome translocations indicates that many but not all of them also have attenuated phenotypes. The mechanism of inactivation of autosomal material attached to the X chromosome is complex, with varying effects on the phenotype of the

  7. High-temperature order-disorder transitions in the skutterudites CoGe{sub 1.5}Q{sub 1.5} (Q=S, Te)

    Energy Technology Data Exchange (ETDEWEB)

    Kaltzoglou, Andreas; Powell, Anthony V. [Institute of Chemical Sciences and Centre for Advanced Energy Storage and Recovery (CAESAR), Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom); Knight, Kevin S. [ISIS Facility, Rutherford Appleton Laboratory, Didcot, Oxfordshire OX11 0OX (United Kingdom); Vaqueiro, Paz, E-mail: chepv@hw.ac.uk [Institute of Chemical Sciences and Centre for Advanced Energy Storage and Recovery (CAESAR), Heriot-Watt University, Edinburgh EH14 4AS (United Kingdom)

    2013-02-15

    The temperature dependence of anion ordering in the skutterudites CoGe{sub 1.5}Q{sub 1.5} (Q=S, Te) has been investigated by powder neutron diffraction. Both materials adopt a rhombohedral structure at room temperature (space group R3{sup Macron} ) in which the anions are ordered trans to each other within Ge{sub 2}Q{sub 2} rings. In CoGe{sub 1.5}S{sub 1.5}, anion ordering is preserved up to the melting point of 950 Degree-Sign C. However, rhombohedral CoGe{sub 1.5}Te{sub 1.5} undergoes a phase transition at 610 Degree-Sign C involving a change to cubic symmetry (space group Im3{sup Macron }). In the high-temperature modification, there is a statistical distribution of anions over the available sites within the Ge{sub 2}Te{sub 2} rings. The structural transition involves a reduction in the degree of distortion of the Ge{sub 2}Te{sub 2} rings which progressively transform from a rhombus to a rectangular shape. The effect of this transition on the thermoelectric properties has been investigated. - Graphical abstract: Powder neutron diffraction reveals that the skutterudite CoGe{sub 1.5}Te{sub 1.5} undergoes a phase transition at 610 Degree-Sign C, involving the disordering of the anions within the Ge{sub 2}Te{sub 2} rings. Highlights: Black-Right-Pointing-Pointer CoGe{sub 1.5}S{sub 1.5} retains an ordered skutterudite structure up to 950 Degree-Sign C. Black-Right-Pointing-Pointer CoGe{sub 1.5}Te{sub 1.5} undergoes an order-disorder phase transition at 610 Degree-Sign C. Black-Right-Pointing-Pointer Below 610 Degree-Sign C, anions are arranged trans to each other within Ge{sub 2}Te{sub 2} rings. Black-Right-Pointing-Pointer Above 610 Degree-Sign C, anions are statistically distributed within the Ge{sub 2}Te{sub 2} rings. Black-Right-Pointing-Pointer The effect of the phase transition on the thermal conductivity is discussed.

  8. Bilateral radial agenesis with absent thumbs, complex heart defect, short stature, and facial dysmorphism in a patient with pure distal microduplication of 5q35.2-5q35.3

    Directory of Open Access Journals (Sweden)

    Jamsheer Aleksander

    2013-01-01

    Full Text Available Abstract Background A partial duplication of the distal long arm of chromosome 5 (5q35-- > qter is known to be associated with a distinct phenotype referred to as Hunter-McAlpine syndrome. Clinical spectrum of this disorder mainly consists of mental retardation, microcephaly, short stature, skeletal anomalies, and craniofacial dysmorphism featuring flat facies, micrognathia, large, low-set dysplastic ears, hypertelorism, almond-shaped, down-slanted palpebral fissures, epicanthal folds, small nose, long philtrum, small mouth, and thin upper lip. Less frequent remarkable findings include craniosynostosis, heart defect, hypoplastic phalanges, preaxial polydactyly, hypospadias, cryptorchidism, and inguinal hernia. In most patients with a partial duplication of 5q the aberration occurred due to an inherited unbalanced translocation, therefore the phenotype was not reflective of pure trisomy 5q. Case presentation We report on a 9.5-year-old boy with some feature of Hunter-McAlpine syndrome including short stature, complex heart defect (dextrocardia, dextroversion, PFO, bilateral cryptorchidism, hypothyroidism, and craniofacial dysmorphism. Additionally, bilateral radial agenesis with complete absence of Ist digital rays, ulnar hypoplasia with bowing, choroidal and retinal coloboma, abnormal biliary vesicle were identified, which have never been noted in 5q trisomy patients. Karyotype analysis, sequencing and MLPA for TBX5 and SALL4 genes were unremarkable. Array comparative genomic hybridization detected a duplication on 5q35.2-5q35.3, resulting from a de novo chromosomal rearrangement. Our proband carried the smallest of all previously reported pure distal 5q trisomies encompassing terminal 5.4-5.6 Mb and presented with the most severe limb malformation attributed to the increased number of distal 5q copies. Conclusions We postulate that a terminal distal trisomy of 5q35.2-5q35.3, which maps 1.1 Mb telomeric to the MSX2 gene is causative for both

  9. Interstitial deletion of 5q33.3q35.1 in a boy with severe mental retardation.

    Science.gov (United States)

    Lee, Jin Hwan; Kim, Hyo Jeong; Yoon, Jung Min; Cheon, Eun Jung; Lim, Jae Woo; Ko, Kyong Og; Lee, Gyung Min

    2016-11-01

    Constitutional interstitial deletions of the long arm of chromosome 5 (5q) are quite rare, and the corresponding phenotype is not yet clearly delineated. Severe mental retardation has been described in most patients who present 5q deletions. Specifically, the interstitial deletion of chromosome 5q33.3q35.1, an extremely rare chromosomal aberration, is characterized by mental retardation, developmental delay, and facial dysmorphism. Although the severity of mental retardation varies across cases, it is the most common feature described in patients who present the 5q33.3q35.1 deletion. Here, we report a case of a de novo deletion of 5q33.3q35.1, 46,XY,del(5)(q33.3q35.1) in an 11-year-old boy with mental retardation; to the best of our knowledge this is the first case in Korea to be reported. He was diagnosed with severe mental retardation, developmental delay, facial dysmorphisms, dental anomalies, and epilepsy. Chromosomal microarray analysis using the comparative genomic hybridization array method revealed a 16-Mb-long deletion of 5q33. 3q35.1(156,409,412-172,584,708)x1. Understanding this deletion may help draw a rough phenotypic map of 5q and correlate the phenotypes with specific chromosomal regions. The 5q33.3q35.1 deletion is a rare condition; however, accurate diagnosis of the associated mental retardation is important to ensure proper genetic counseling and to guide patients as part of long-term management.

  10. Clinical phenotype and candidate genes for the 5q31.3 microdeletion syndrome.

    Science.gov (United States)

    Hosoki, Kana; Ohta, Tohru; Natsume, Jun; Imai, Sumiko; Okumura, Akihisa; Matsui, Takeshi; Harada, Naoki; Bacino, Carlos A; Scaglia, Fernando; Jones, Jeremy Y; Niikawa, Norio; Saitoh, Shinji

    2012-08-01

    Array-based technologies have led to the identification of many novel microdeletion and microduplication syndromes demonstrating multiple congenital anomalies and intellectual disability (MCA/ID). We have used chromosomal microarray analysis for the evaluation of patients with MCA/ID and/or neonatal hypotonia. Three overlapping de novo microdeletions at 5q31.3 with the shortest region of overlap (SRO) of 370 kb were detected in three unrelated patients. These patients showed similar clinical features including severe neonatal hypotonia, neonatal feeding difficulties, respiratory distress, characteristic facial features, and severe developmental delay. These features are consistent with the 5q31.3 microdeletion syndrome originally proposed by Shimojima et al., providing further evidence that this syndrome is clinically discernible. The 370 kb SRO encompasses only four RefSeq genes including neuregulin 2 (NRG2) and purine-rich element binding protein A (PURA). NRG2 is one of the members of the neuregulin family related to neuronal and glial cell growth and differentiation, thus making NRG2 a good candidate for the observed phenotype. Moreover, PURA is also a good candidate because Pura-deficient mice demonstrate postnatal neurological manifestations.

  11. The gene for human glutaredoxin (GLRX) is localized to human chromosome 5q14

    Energy Technology Data Exchange (ETDEWEB)

    Padilla, C.A.; Holmgren, A. [Karolinska Inst., Stockholm (Sweden); Bajalica, S.; Lagercrantz, J. [Karolinska Hospital, Stockholm (Sweden)

    1996-03-05

    Glutaredoxin is a small protein (12 kDa) catalyzing glutathione-dependent disulfide oxidoreduction reactions in a coupled system with NADPH, GSH, and glutathione reductase. A cDNA encoding the human glutaredoxin gene (HGMW-approved symbol GLRX) has recently been isolated and cloned from a human fetal spleen cDNA library. The screening of a human fetal spleen cDNA library. The screening of a human genomic library in Charon 4A led to the identification of three genomic clones. Using fluorescence in situ hybridization to metaphase chromosomes with one genomic clone as a probe, the human glutaredoxin gene was localized to chromosomal region 5q14. This localization at chromosome 5 was in agreement with the somatic cell hybrid analysis, using DNA from a human-hamster and a human-mouse hybrid panel and using a human glutaredoxin cDNA as a probe. 13 refs., 2 figs.

  12. Variation in conserved non-coding sequences on chromosome 5q andsusceptibility to asthma and atopy

    Energy Technology Data Exchange (ETDEWEB)

    Donfack, Joseph; Schneider, Daniel H.; Tan, Zheng; Kurz,Thorsten; Dubchak, Inna; Frazer, Kelly A.; Ober, Carole

    2005-09-10

    Background: Evolutionarily conserved sequences likely havebiological function. Methods: To determine whether variation in conservedsequences in non-coding DNA contributes to risk for human disease, westudied six conserved non-coding elements in the Th2 cytokine cluster onhuman chromosome 5q31 in a large Hutterite pedigree and in samples ofoutbred European American and African American asthma cases and controls.Results: Among six conserved non-coding elements (>100 bp,>70percent identity; human-mouse comparison), we identified one singlenucleotide polymorphism (SNP) in each of two conserved elements and sixSNPs in the flanking regions of three conserved elements. We genotypedour samples for four of these SNPs and an additional three SNPs each inthe IL13 and IL4 genes. While there was only modest evidence forassociation with single SNPs in the Hutterite and European Americansamples (P<0.05), there were highly significant associations inEuropean Americans between asthma and haplotypes comprised of SNPs in theIL4 gene (P<0.001), including a SNP in a conserved non-codingelement. Furthermore, variation in the IL13 gene was strongly associatedwith total IgE (P = 0.00022) and allergic sensitization to mold allergens(P = 0.00076) in the Hutterites, and more modestly associated withsensitization to molds in the European Americans and African Americans (P<0.01). Conclusion: These results indicate that there is overalllittle variation in the conserved non-coding elements on 5q31, butvariation in IL4 and IL13, including possibly one SNP in a conservedelement, influence asthma and atopic phenotypes in diversepopulations.

  13. A YAC contig of approximately 3 Mb from human chromosome 5q31 [yields] q33

    Energy Technology Data Exchange (ETDEWEB)

    Li, Xiang; Wang Jabs, E.; Hawkins, A.L.; Griffin, C.A. (John Hopkins School of Medicine, Baltimore, MD (United States)); Wise, C.A.; Lovett, M. (Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)); Le Paslier, D. (CEPH, Paris (France)); Pittler, S.J. (Univ. of South Alabama College of Medicine, Mobile, AL (United States))

    1994-02-01

    The human chromosome 5q31-q33 region contains an interesting cluster of growth factor and receptor genes. In addition, several genetic disease loci have been localized within this region, but have not as yet been isolated as molecular clones. These include those loci involved in autosomal dominant limb-girdle muscular dystrophy, diastrophic dysplasia, Treacher Collins syndrome, and myeloid disorders associated with the 5q-syndrome. A yeast artificial chromosome (YAC) contig of this region would assist in the further localization and isolation of these genes. The authors have used YACs isolated from the Washington University and Centre d'Etude du Polymorphisme Humain YAC libraries, including YACs from the large insert (mega) YAC library to build a contig greater than 3 Mb in size. An STS content strategy coupled with limited walking from YAC ends was used to isolate 22 overlapping YACs with as much as sixfold coverage. A total of 20 STSs, derived from genes, anonymous sequences, and vector Alu-PCR or inverse PCR products, were used to compile this contig. The order of loci, centromere-GRL-D5S207-D5S70-D5S545-D5S546-D5S547-D5S68-D5S548-D5S210-D5S549-D5S686- ADRB2-D5S559-CSF1R-D5S551-RPS14-D5S519-SPARC-telomere, was derived from the overlapping clones. This contig and clones derived from it will be useful substrates in selecting candidate cDNAs for the disease loci in this interval. 45 refs., 1 fig., 2 tabs.

  14. Targeted re-sequencing analysis of 25 genes commonly mutated in myeloid disorders in del(5q) myelodysplastic syndromes

    Science.gov (United States)

    Fernandez-Mercado, Marta; Burns, Adam; Pellagatti, Andrea; Giagounidis, Aristoteles; Germing, Ulrich; Agirre, Xabier; Prosper, Felipe; Aul, Carlo; Killick, Sally; Wainscoat, James S.; Schuh, Anna; Boultwood, Jacqueline

    2013-01-01

    Interstitial deletion of chromosome 5q is the most common chromosomal abnormality in myelodysplastic syndromes. The catalogue of genes involved in the molecular pathogenesis of myelodysplastic syndromes is rapidly expanding and next-generation sequencing technology allows detection of these mutations at great depth. Here we describe the design, validation and application of a targeted next-generation sequencing approach to simultaneously screen 25 genes mutated in myeloid malignancies. We used this method alongside single nucleotide polymorphism-array technology to characterize the mutational and cytogenetic profile of 43 cases of early or advanced del(5q) myelodysplastic syndromes. A total of 29 mutations were detected in our cohort. Overall, 45% of early and 66.7% of advanced cases had at least one mutation. Genes with the highest mutation frequency among advanced cases were TP53 and ASXL1 (25% of patients each). These showed a lower mutation frequency in cases of 5q- syndrome (4.5% and 13.6%, respectively), suggesting a role in disease progression in del(5q) myelodysplastic syndromes. Fifty-two percent of mutations identified were in genes involved in epigenetic regulation (ASXL1, TET2, DNMT3A and JAK2). Six mutations had allele frequencies <20%, likely below the detection limit of traditional sequencing methods. Genomic array data showed that cases of advanced del(5q) myelodysplastic syndrome had a complex background of cytogenetic aberrations, often encompassing genes involved in myeloid disorders. Our study is the first to investigate the molecular pathogenesis of early and advanced del(5q) myelodysplastic syndromes using next-generation sequencing technology on a large panel of genes frequently mutated in myeloid malignancies, further illuminating the molecular landscape of del(5q) myelodysplastic syndromes. PMID:23831921

  15. Lenalidomide induces cell death in an MDS-derived cell line with deletion of chromosome 5q by inhibition of cytokinesis

    National Research Council Canada - National Science Library

    Matsuoka, A; Tochigi, A; Kishimoto, M; Nakahara, T; Kondo, T; Tsujioka, T; Tasaka, T; Tohyama, Y; Tohyama, K

    2010-01-01

    ... Organization classification of MDS. (4) Although the deleted area of 5q is different in case by case, del(5)(q32q33) is considered as a common deleted region. (5,6) The common deleted region expected in patients with 5q- syndrome is located distal to the region recognized in higher-risk groups with del(5q) that are susceptible to leuk...

  16. Coexistent t(8;21)(q22;q22) Translocation and 5q Deletion in Acute Myeloid Leukemia.

    Science.gov (United States)

    Yamamoto, Katsuya; Yakushijin, Kimikazu; Sanada, Yukinari; Kawamoto, Shinichiro; Matsuoka, Hiroshi; Minami, Hironobu

    2015-01-01

    The t(8;21)(q22;q22) translocation is specifically observed in acute myeloid leukemia (AML) M2 subtype, whereas del(5q) is one of the most common cytogenetic aberrations in myelodysplastic syndromes (MDS). Thus, t(8;21)(q22;q22) and del(5q) appear to be mutually exclusive, and the association between them has not been characterized yet. Here, we report an 81-year-old woman with coexistent t(8;21)(q22;q22) and del(5q) at initial diagnosis. The bone marrow was infiltrated with 18.4% myeloblasts, and showed marked myeloid and erythroid dysplasia. Myeloblasts were positive for CD19 and CD56 as well as CD13, CD33, CD34 and HLA-DR. G-banding and spectral karyotyping showed 46,XX,del(5)(q?),t(8;21)(q22;q22)[18]/46,XX[2]. Both del(5)(q?) and t(8;21)(q22;q22) were present in a single clone. Fluorescence in situ hybridization (FISH) on metaphase spreads detected a RUNX1/RUNX1T1 fusion signal on the der(8)t(8;21)(q22;q22), and confirmed deletion of CSF1R signaling at 5q33-q34 on the del(5)(q?). Furthermore, FISH on interphase nuclei revealed that the RUNX1/RUNX1T1 fusion signal and deletion of CSF1R signaling were found in 66.0% and 58.0% of interphase cells, respectively, suggesting that del(5)(q?) occurred in cells with RUNX1/RUNX1T1. These results indicated a diagnosis of AML with t(8;21)(q22;q22)/RUNX1/RUNX1T1 rather than MDS, even though the percentage of bone marrow myeloblasts was less than 20%. Based on these findings, together with those of other reported cases, del(5q) seems to be an extremely rare but recurrent secondary aberration in AML with t(8;21)(q22;q22).

  17. Discovery of Genetic Variation on Chromosome 5q22 Associated with Mortality in Heart Failure.

    Directory of Open Access Journals (Sweden)

    J Gustav Smith

    2016-05-01

    Full Text Available Failure of the human heart to maintain sufficient output of blood for the demands of the body, heart failure, is a common condition with high mortality even with modern therapeutic alternatives. To identify molecular determinants of mortality in patients with new-onset heart failure, we performed a meta-analysis of genome-wide association studies and follow-up genotyping in independent populations. We identified and replicated an association for a genetic variant on chromosome 5q22 with 36% increased risk of death in subjects with heart failure (rs9885413, P = 2.7x10-9. We provide evidence from reporter gene assays, computational predictions and epigenomic marks that this polymorphism increases activity of an enhancer region active in multiple human tissues. The polymorphism was further reproducibly associated with a DNA methylation signature in whole blood (P = 4.5x10-40 that also associated with allergic sensitization and expression in blood of the cytokine TSLP (P = 1.1x10-4. Knockdown of the transcription factor predicted to bind the enhancer region (NHLH1 in a human cell line (HEK293 expressing NHLH1 resulted in lower TSLP expression. In addition, we observed evidence of recent positive selection acting on the risk allele in populations of African descent. Our findings provide novel genetic leads to factors that influence mortality in patients with heart failure.

  18. Faithful expression of the human 5q31 cytokine cluster intransgenic mice

    Energy Technology Data Exchange (ETDEWEB)

    Lacy, Dee A.; Wang, Zhi-En; Symula, Derek J.; McArthur, CliffordJ.; Rubin, Edward M.; Frazer, Kelly A.; Locksley, Richard M.

    1999-12-03

    ILs 4,5, and 13, cardinal cytokines produced by Th2 cells,are coordinately expressed and clustered in the 150-kb syntenic regions on mouse chromosome 11 and human chromosome 5q31. We analyzed two sets of human yeast artificial chromosome transgenic mice that contained the5931cytokines to assess whether conserved sequences required for their coordinate and cell-specific regulation are contained within the cytokine cluster itself. Human Il-4, IL-13, and Il-5 were expressed under Th2, but not Th1, conditions in vitro. Each of these cytokines was produced during infection with Nippostrongylus brasiliensis, a Th2 inducing stimulus, and human Il-4 was generated after activation of NK T cells in vivo.Consistently fewer cells produced the endogenous mouse cytokines in transgenic than in control mice, suggesting competition for stable expression between the mouse and human genes. These data imply the existence of both conserved trans-activating factors and cis-regulatory elements that underlie the coordinate expression and lineage specificity of the type 2 ctyokine genes in lymphocytes.

  19. A Patient with Interstitial 5q21 Deletion, Familial Adenomatous Polyposis, Dysmorphic Features, and Profound Neurologic Dysfunction

    Directory of Open Access Journals (Sweden)

    Manoochehr Karjoo

    2017-01-01

    Full Text Available Familial adenomatous polyposis (FAP is a hereditary autosomal dominant cancer syndrome, results from germ line mutation or deletion of the Adenomatous Polyposis Coli (APC gene on chromosome 5q21. Patients with FAP suffer from multiple polyps mainly at the colorectal region as well as other parts of the gastrointestinal tract, which has propensity to transform into carcinoma. FAP has also been well described in association with various syndromic extra-gastrointestinal manifestations. Less commonly, patients with FAP present with varying degrees of cognitive dysfunction and developmental delay, though the reason for the association is unclear. Herein, we report the case of a male patient born with an interstitial deletion of chromosome 5q, 46,XY, del(5 (q14q23, presenting with familial adenomatous polyposis (FAP, profound developmental delay, cognitive dysfunction, and multiple congenital anomalies including talipes equinovarus, agenesis of the corpus callosum, and dysmorphic facial features.

  20. Genetic Predisposition to Multiple Myeloma at 5q15 Is Mediated by an ELL2 Enhancer Polymorphism

    Directory of Open Access Journals (Sweden)

    Ni Li

    2017-09-01

    Full Text Available Multiple myeloma (MM is a malignancy of plasma cells. Genome-wide association studies have shown that variation at 5q15 influences MM risk. Here, we have sought to decipher the causal variant at 5q15 and the mechanism by which it influences tumorigenesis. We show that rs6877329 G > C resides in a predicted enhancer element that physically interacts with the transcription start site of ELL2. The rs6877329-C risk allele is associated with reduced enhancer activity and lowered ELL2 expression. Since ELL2 is critical to the B cell differentiation process, reduced ELL2 expression is consistent with inherited genetic variation contributing to arrest of plasma cell development, facilitating MM clonal expansion. These data provide evidence for a biological mechanism underlying a hereditary risk of MM at 5q15.

  1. The association between DNA copy number aberrations at chromosome 5q22 and gastric cancer.

    Directory of Open Access Journals (Sweden)

    Pei-Chien Tsai

    Full Text Available BACKGROUND: Gastric cancer is common cancer. Discovering novel genetic biomarkers might help to identify high-risk individuals. Copy number variation (CNV has recently been shown to influence risk for several cancers. The aim of the present study was sought to test the association between copy number at a variant region and GC. METHODS: A total of 110 gastric cancer patients and 325 healthy volunteers were enrolled in this study. We searched for a CNV and found a CNV (Variation 7468 containing part of the APC gene, the SRP19 gene and the REEP5 gene. We chose four probes targeting at APC-intron8, APC-exon9, SRP19 and REEP5 to interrogate this CNV. Specific Taqman probes labeled by different reporter fluorophores were used in a real-time PCR platform to obtain copy number. Both the original non-integer data and transformed integer data on copy number were used for analyses. RESULTS: Gastric caner patients had a lower non-integer copy number than controls for the APC-exon9 probe (Adjusted p = 0.026 and SRP19 probe (Adjusted p = 0.002. The analysis of integer copy number yielded a similar pattern although less significant (Adjusted p = 0.07 for APC-exon9 probe and Adjusted p = 0.02 for SRP19 probe. CONCLUSIONS: Losses of a CNV at 5q22, especially in the DNA region surrounding APC-exon 9, may be associated with a higher risk of gastric cancer.

  2. A Locus at 5q33.3 Confers Resistance to Tuberculosis in Highly Susceptible Individuals

    Science.gov (United States)

    Sobota, Rafal S.; Stein, Catherine M.; Kodaman, Nuri; Scheinfeldt, Laura B.; Maro, Isaac; Wieland-Alter, Wendy; Igo, Robert P.; Magohe, Albert; Malone, LaShaunda L.; Chervenak, Keith; Hall, Noemi B.; Modongo, Chawangwa; Zetola, Nicola; Matee, Mecky; Joloba, Moses; Froment, Alain; Nyambo, Thomas B.; Moore, Jason H.; Scott, William K.; Lahey, Timothy; Boom, W. Henry; von Reyn, C. Fordham; Tishkoff, Sarah A.; Sirugo, Giorgio; Williams, Scott M.

    2016-01-01

    Immunosuppression resulting from HIV infection increases the risk of progression to active tuberculosis (TB) both in individuals newly exposed to Mycobacterium tuberculosis (MTB) and in those with latent infections. We hypothesized that HIV-positive individuals who do not develop TB, despite living in areas where it is hyperendemic, provide a model of natural resistance. We performed a genome-wide association study of TB resistance by using 581 HIV-positive Ugandans and Tanzanians enrolled in prospective cohort studies of TB; 267 of these individuals developed active TB, and 314 did not. A common variant, rs4921437 at 5q33.3, was significantly associated with TB (odds ratio = 0.37, p = 2.11 × 10−8). This variant lies within a genomic region that includes IL12B and is embedded in an H3K27Ac histone mark. The locus also displays consistent patterns of linkage disequilibrium across African populations and has signals of strong selection in populations from equatorial Africa. Along with prior studies demonstrating that therapy with IL-12 (the cytokine encoded in part by IL12B, associated with longer survival following MTB infection in mice deficient in CD4 T cells), our results suggest that this pathway might be an excellent target for the development of new modalities for treating TB, especially for HIV-positive individuals. Our results also indicate that studying extreme disease resistance in the face of extensive exposure can increase the power to detect associations in complex infectious disease. PMID:26942285

  3. New superhindered polydentate polyphosphine ligands P(CH2CH2P(t)Bu2)3, PhP(CH2CH2P(t)Bu2)2, P(CH2CH2CH2P(t)Bu2)3, and their ruthenium(II) chloride complexes.

    Science.gov (United States)

    Gilbert-Wilson, Ryan; Field, Leslie D; Bhadbhade, Mohan M

    2012-03-05

    The synthesis and characterization of the extremely hindered phosphine ligands, P(CH(2)CH(2)P(t)Bu(2))(3) (P(2)P(3)(tBu), 1), PhP(CH(2)CH(2)P(t)Bu(2))(2) (PhP(2)P(2)(tBu), 2), and P(CH(2)CH(2)CH(2)P(t)Bu(2))(3) (P(3)P(3)(tBu), 3) are reported, along with the synthesis and characterization of ruthenium chloro complexes RuCl(2)(P(2)P(3)(tBu)) (4), RuCl(2)(PhP(2)P(2)(tBu)) (5), and RuCl(2)(P(3)P(3)(tBu)) (6). The bulky P(2)P(3)(tBu) (1) and P(3)P(3)(tBu) (3) ligands are the most sterically encumbered PP(3)-type ligands so far synthesized, and in all cases, only three phosphorus donors are able to bind to the metal center. Complexes RuCl(2)(PhP(2)P(2)(tBu)) (5) and RuCl(2)(P(3)P(3)(tBu)) (6) were characterized by crystallography. Low temperature solution and solid state (31)P{(1)H} NMR were used to demonstrate that the structure of RuCl(2)(P(2)P(3)(tBu)) (4) is probably analogous to that of RuCl(2)(PhP(2)P(2)(tBu)) (5) which had been structurally characterized.

  4. p53 protein expression independently predicts outcome in patients with lower-risk myelodysplastic syndromes with del(5q)

    NARCIS (Netherlands)

    Saft, L.; Karimi, M.; Ghaderi, M.; Matolcsy, A.; Mufti, G.J.; Kulasekararaj, A.; Gohring, G.; Giagounidis, A.; Selleslag, D.; Muus, P.; Sanz, G.; Mittelman, M.; Bowen, D.; Porwit, A.; Fu, T.; Backstrom, J.; Fenaux, P.; MacBeth, K.J.; Hellstrom-Lindberg, E.

    2014-01-01

    Del(5q) myelodysplastic syndromes defined by the International Prognostic Scoring System as low- or intermediate-1-risk (lower-risk) are considered to have an indolent course; however, recent data have identified a subgroup of these patients with more aggressive disease and poorer outcomes. Using de

  5. Analysis of the efficacy of lenalidomide in patients with intermediate-1 risk myelodysplastic syndrome without 5q deletion.

    Science.gov (United States)

    Yang, Yan; Gao, Sujun; Fan, Hongqiong; Lin, Hai; Li, Wei; Wang, Juan

    2013-09-01

    The aim of this study was to evaluate the efficacy and adverse effects of lenalidomide in the treatment of intermediate-1 risk non-5q deletion [non-del (5q)] myelodysplastic syndrome (MDS). A total of 30 patients with MDS were classified through G-banding chromosome karyotype analysis and fluorescence in situ hybridization (FISH). According to the International Prognostic Scoring System scores, among the 30 patients, 23 and seven cases had scores of 0.5 and 1.0, respectively. Lenalidomide (Revlimid(®)), 10 mg/day) was administered for 21 days every 28 days. All 30 cases were treated with lenalidomide for at least three cycles, including 20 cases with four cycles. The patients did not require erythropoietin, cyclosporine or iron chelation treatments. Statistical analysis was performed using SPSS statistical software version 13.0, and comparisons among groups were conducted using a t-test. The efficacy of lenalidomide was demonstrated in patients with intermediate-1 risk non-del (5q) MDS. Peripheral blood cell counts were improved following treatment, and absolute neutrophil, haemoglobin and platelet counts increased following 2-4 cycles of treatment. All patients became stable having undergone three cycles of treatment; however, 17 patients with chromosomal abnormalities had no cytogenetic response to the treatment, as confirmed through the FISH test. Patients with intermediate-1 risk non-del (5q) MDS treated with lenalidomide did not achieve complete haematological remission, although they demonstrated haematological improvement.

  6. O(^3 p) Doped Helium Droplets

    Science.gov (United States)

    Brice, Joseph T.; Douberly, Gary E.

    2017-06-01

    Atomic oxygen (^3 P) is generated via thermolysis in a commerical thermal gas cracker (Mantis Ltd. MGC-75). Complexes with HCN were investigated to qualitatively assess the doping efficiency of O(^3 P) into a helium droplet. Theoretical calculations of a linear O \\cdot\\cdot\\cdot HCN (^3 Σ) complex at the CCSD(T)/aug-cc-pVTZ level are consistent with the rotational constants extracted from the rotational substructure in the experimental spectra, and with dipole moments approximated from Stark spectra. The thermal source will be used to study reactions between O(^3 P) and hydrocarbons in helium droplets, and preliminary data on this topic will be presented.

  7. Cytogenetic and molecular predictors of response in patients with myeloid malignancies without del[5q] treated with lenalidomide

    Directory of Open Access Journals (Sweden)

    Sugimoto Yuka

    2012-03-01

    Full Text Available Abstract Background While lenalidomide (LEN shows high efficacy in myelodysplastic syndromes (MDS with del[5q], responses can be also seen in patients presenting without del[5q]. We hypothesized that improved detection of chromosomal abnormalities with new karyotyping tools may better predict response to LEN. Design and methods We have studied clinical, molecular and cytogenetic features of 42 patients with MDS, myeloproliferative neoplasms (MPN, MDS/MPN overlap syndromes and secondary acute myeloid leukemia (sAML without del[5q] by metaphase cytogenetics (MC who underwent therapy with LEN. Results Fluorescence in situ hybridization (FISH or single nucleotide polymorphism array (SNP-A-based karyotyping marginally increased the diagnostic yield over MC, detecting 2/42 (4.8% additional cases with del[5q], one of whom were responded to LEN. Responses were more often observed in patients with a normal karyotype by MC (60% vs abnormal MC; 17%, p = .08 and those with gain of chromosome 8 material by either of all 3 karyotyping methods (83% vs all other chromosomal abnormalities; 44% p = .11. However, 5 out of those 6 patients received combined LEN/AZA therapy and it may also suggest those with gain of chromosome 8 material respond well to AZA. The addition of FISH or SNP-A did not improve the predictive value of normal cytogenetics by MC. Mutational analysis of TET2, UTX, CBL, EZH2, ASXL1, TP53, RAS, IDH1/2, and DNMT-3A was performed on 21 of 41 patients, and revealed 13 mutations in 11 patients, but did not show any molecular markers of responsiveness to LEN. Conclusions Normal karyotype and gain of chromosome 8 material was predictive of response to LEN in non-del[5q] patients with myeloid malignancies.

  8. Mass-dependent dynamics of the luminescent exchange reactions C+(2P), P+(3P) + H2, D2, HD

    Science.gov (United States)

    Glenewinkel-Meyer, Th; Hoppe, U.; Kowalski, A.; Ottinger, Ch; Rabenda, D.

    1995-06-01

    Chemiluminescent ion/molecule reactions of ground state C+ and P+ ions with H2, D2 and HD have been studied in an ion beam/target gas cell arrangement. Emission spectra of CH+, CD+ (A 1II) and of PH+, PD+ (A 2[Delta]) were observed with up to 1 Å FWHM resolution and at collision energies from threshold ([approximate] 3 eV) to 8 eVc.m. (centre-of-mass) and 15eVc.m., respectively. Very detailed computer simulations of the spectral contours were done, including ab initio transition moments and, in the case of PH+/PD+, the effects of predissociation. In simulating the spectra obtained with HD, the overlapped hydride and deuteride product ion spectra could be isolated by varying the respective weighting factors to achieve an optimum overall fit. In the case of C+ + HD, the two components were found to have very similar rovibrational distributions as with the products from C+ + H2 and C+ + D2. In the P+ case, however, the rotational, although not the vibrational, distributions were found to be significantly different for the isotopically mixed and the pure reactions. The cross-sections showed an intermolecular isotope effect only for C+ + H2 vs. C+ + D2 at high energies. However, both with C+ + HD and P+ + HD, a very strong intramolecular isotope effect, i.e. an energy-dependent branching ratio, was observed: at low energies deuteride formation prevails, at high energies hydride. This behaviour is discussed in terms of an impulsive collision model, assuming the "pairwise" relative kinetic energy between the reacting atoms to be the determining factor. On the basis of the measured cross-section curves for the H2 and D2 reactions, the energy-dependent hydride/deuteride ratio in the HD reaction can then be predicted. The agreement with the experimental results is excellent in the P+ case, but only moderate for the C+ reactions. Even the P+ reaction, however, does not occur via the spectator stripping mechanisms. The spectra show an energy-independent vibrational excitation, contrary to the stripping model assumption. The impulsive model was also extended to an analysis of the relative mean product angular momenta. Again it performs well for P+ + H2, D2, HD, but cannot fit the C+ + HD case.

  9. A novel 5q11.2 microdeletion in a child with mild developmental delay and dysmorphic features.

    Science.gov (United States)

    Fontana, Paolo; Tortora, Cristina; Petillo, Roberta; Falco, Mariateresa; Miniero, Martina; De Brasi, Davide; Pisanti, Maria Antonietta

    2016-09-01

    5q11.2 Deletion is a very rare genomic disorder, and its clinical phenotype has not yet been characterized. This report describes a patient with an 8.6 Mb deletion, showing hypotonia, mild developmental delay, short stature, and distinctive dysmorphic features (frontal bossing, square face, deep-set eyes, prominent columella, long philtrum, thin lips). © 2016 Wiley Periodicals, Inc.

  10. Thermoelastic properties of Zn3P2

    DEFF Research Database (Denmark)

    Gerward, Leif; Olsen, J. Staun; Waśkowska, A.

    2011-01-01

    The bulk modulus and thermal expansion of Zn3P2 has been investigated at pressures up to 21GPa and temperatures down to 100K. The experimental zero-pressure bulk modulus is 80.7 ± 1.8GPa, in accordance with the bulk modulus scaling and lattice properties of the related compound Cd3P2. A tetragonal...... to orthorhombic phase transformation occurs above 11GPa with a relative volume change of-7.1%. Values for the thermal expansion coefficient are reported at 293, 200 and 100K....

  11. A syndromic form of Pierre Robin sequence is caused by 5q23 deletions encompassing FBN2 and PHAX.

    Science.gov (United States)

    Ansari, Morad; Rainger, Jacqueline K; Murray, Jennie E; Hanson, Isabel; Firth, Helen V; Mehendale, Felicity; Amiel, Jeanne; Gordon, Christopher T; Percesepe, Antonio; Mazzanti, Laura; Fryer, Alan; Ferrari, Paola; Devriendt, Koenraad; Temple, I Karen; FitzPatrick, David R

    2014-10-01

    Pierre Robin sequence (PRS) is an aetiologically distinct subgroup of cleft palate. We aimed to define the critical genomic interval from five different 5q22-5q31 deletions associated with PRS or PRS-associated features and assess each gene within the region as a candidate for the PRS component of the phenotype. Clinical array-based comparative genome hybridisation (aCGH) data were used to define a 2.08 Mb minimum region of overlap among four de novo deletions and one mother-son inherited deletion associated with at least one component of PRS. Commonly associated anomalies were talipes equinovarus (TEV), finger contractures and crumpled ear helices. Expression analysis of the orthologous genes within the PRS critical region in embryonic mice showed that the strongest candidate genes were FBN2 and PHAX. Targeted aCGH of the critical region and sequencing of these genes in a cohort of 25 PRS patients revealed no plausible disease-causing mutations. In conclusion, deletion of ∼2 Mb on 5q23 region causes a clinically recognisable subtype of PRS. Haploinsufficiency for FBN2 accounts for the digital and auricular features. A possible critical region for TEV is distinct and telomeric to the PRS region. The molecular basis of PRS in these cases remains undetermined but haploinsufficiency for PHAX is a plausible mechanism.

  12. Fine-scale mapping of the 5q11.2 breast cancer locus reveals at least three independent risk variants regulating MAP3K1

    NARCIS (Netherlands)

    D.M. Glubb (Dylan); M. Maranian (Melanie); K. Michailidou (Kyriaki); K.A. Pooley (Karen); K.B. Meyer (Kerstin); S. Kar (Siddhartha); A.F.C. Carlebur; M. O'Reilly (Marian); J.A. Betts (Joshua); K.M. Hillman (Kristine); S. Kaufmann (Susanne); J. Beesley (Jonathan); S. Canisius (Sander); J.L. Hopper (John); M.C. Southey (Melissa); H. Tsimiklis (Helen); C. Apicella (Carmel); M.K. Schmidt (Marjanka); A. Broeks (Annegien); F.B.L. Hogervorst (Frans); C.E. van der Schoot (Ellen); K.R. Muir (K.); A. Lophatananon (Artitaya); S. Stewart-Brown (Sarah); P. Siriwanarangsan (Pornthep); P.A. Fasching (Peter); M. Ruebner (Matthias); A.B. Ekici (Arif); M.W. Beckmann (Matthias W.); J. Peto (Julian); I. dos Santos Silva (Isabel); O. Fletcher (Olivia); N. Johnson (Nichola); P.D.P. Pharoah (Paul D.P.); M.K. Bolla (Manjeet); Q. Wang (Qing); J. Dennis (Joe); E.J. Sawyer (Elinor); I.P. Tomlinson (Ian); M. Kerin (Michael); N. Miller (Nicola); B. Burwinkel (Barbara); F. Marme (Federick); R. Yang (Rongxi); H. Surowy (Harald); P. Guénel (Pascal); T. Truong (Thérèse); F. Menegaux (Florence); M. Sanchez (Marie); S.E. Bojesen (Stig); B.G. Nordestgaard (Børge); S.F. Nielsen (Sune); H. Flyger (Henrik); A. González-Neira (Anna); J. Benítez (Javier); M.P. Zamora (Pilar); J.I. Arias Pérez (José Ignacio); H. Anton-Culver (Hoda); S.L. Neuhausen (Susan); H. Brenner (Hermann); A.K. Dieffenbach (Aida Karina); V. Arndt (Volker); C. Stegmaier (Christa); A. Meindl (Alfons); R.K. Schmutzler (Rita); H. Brauch (Hiltrud); Y.-D. Ko (Yon-Dschun); T. Brüning (Thomas); H. Nevanlinna (Heli); T.A. Muranen (Taru); K. Aittomäki (Kristiina); C. Blomqvist (Carl); K. Matsuo (Keitaro); H. Ito (Hidemi); H. Iwata (Hisato); H. Tanaka (Hideo); T. Dörk (Thilo); N.V. Bogdanova (Natalia); S. Helbig (Sonja); A. Lindblom (Annika); S. Margolin (Sara); A. Mannermaa (Arto); V. Kataja (Vesa); V-M. Kosma (Veli-Matti); J.M. Hartikainen (J.); A.H. Wu (Anna H.); C.-C. Tseng (Chiu-Chen); D. Van Den Berg (David); D.O. Stram (Daniel O.); D. Lambrechts (Diether); H. Zhao (Hui); C. Weltens (Caroline); E. van Limbergen (Erik); J. Chang-Claude (Jenny); D. Flesch-Janys (Dieter); A. Rudolph (Anja); P. Seibold (Petra); P. Radice (Paolo); P. Peterlongo (Paolo); M. Barile (Monica); F. Capra (Fabio); F.J. Couch (Fergus); J.E. Olson (Janet); B. Hallberg (Boubou); C. Vachon (Celine); G.G. Giles (Graham); R.L. Milne (Roger); C.A. McLean (Catriona Ann); C.A. Haiman (Christopher); B.E. Henderson (Brian); F.R. Schumacher (Fredrick); L. Le Marchand (Loic); J. Simard (Jacques); M.S. Goldberg (Mark); F. Labrèche (France); M. Dumont (Martine); S.-H. Teo; C.H. Yip (Cheng Har); M.-H. See (Mee-Hoong); B.K. Cornes (Belinda); C.-Y. Cheng (Ching-Yu); M.K. Ikram (Kamran); V. Kristensen (Vessela); W. Zheng (Wei); S.L. Halverson (Sandra L.); M. Shrubsole (Martha); J. Long (Jirong); R. Winqvist (Robert); K. Pykäs (Katri); A. Jukkola-Vuorinen (Arja); S. Kauppila (Saila); I.L. Andrulis (Irene); J.A. Knight (Julia); G. Glendon (Gord); S. Tchatchou (Sandrine); P. Devilee (Peter); R.A.E.M. Tollenaar (Rob); C.M. Seynaeve (Caroline); C.J. van Asperen (Christi); M. García-Closas (Montserrat); J.D. Figueroa (Jonine); S.J. Chanock (Stephen); J. Lissowska (Jolanta); K. Czene (Kamila); D. Klevebring (Daniel); H. Darabi (Hatef); M. Eriksson (Mikael); M.J. Hooning (Maartje); A. Hollestelle (Antoinette); J.W.M. Martens (John W. M.); J.M. Collee (Margriet); P. Hall (Per); J. Li (Jingmei); K. Humphreys (Keith); X.-O. Shu (Xiao-Ou); W. Lu (Wei); Y.-T. Gao (Yu-Tang); H. Cai (Hui); A. Cox (Angela); S.S. Cross (Simon); M.W.R. Reed (Malcolm); W.J. Blot (William); L.B. Signorello (Lisa B.); Q. Cai (Qiuyin); M. Shah (Mitul); M. Ghoussaini (Maya); D. Kang (Daehee); J.-Y. Choi (J.); S.K. Park (Sue); D-Y. Noh (Dong-Young); M. Hartman (Mikael); X. Miao; W.-Y. Lim (Wei-Yen); A. Tang (Anthony); U. Hamann (Ute); D. Torres (Diana); A. Jakubowska (Anna); J. Lubinski (Jan); K. Jaworska (Katarzyna); K. Durda (Katarzyna); S. Sangrajrang (Suleeporn); V. Gaborieau (Valerie); P. Brennan (Paul); J.D. McKay (James); C. Olswold (Curtis); S. Slager (Susan); A.E. Toland (Amanda); D. Yannoukakos (Drakoulis); C-Y. Shen (Chen-Yang); P.-E. Wu (Pei-Ei); J-C. Yu (Jyh-Cherng); M.-F. Hou (Ming-Feng); A.J. Swerdlow (Anthony ); A. Ashworth (Alan); N. Orr (Nick); M. Jones (Michael); G. Pita (G.); M.R. Alonso (M Rosario); N. Álvarez (Nuria); D. Herrero (Daniel); D.C. Tessier (Daniel C.); D. Vincent (Daniel); F. Bacot (Francois); C. Luccarini (Craig); C. Baynes (Caroline); S. Ahmed (Shahana); S. Healey (Sue); M. Brown (Melissa); B.A.J. Ponder (Bruce A.J.); G. Chenevix-Trench (Georgia); D. Thompson (Deborah); S.L. Edwards (Stacey); D.F. Easton (Douglas); A.M. Dunning (Alison); J.D. French (Juliet)

    2015-01-01

    textabstractGenome-wide association studies (GWASs) have revealed SNP rs889312 on 5q11.2 to be associated with breast cancer risk in women of European ancestry. In an attempt to identify the biologically relevant variants, we analyzed 909 genetic variants across 5q11.2 in 103,991 breast cancer indiv

  13. Definition of 5q11.2 Microdeletion Syndrome Reveals Overlap with CHARGE Syndrome and 22q11 Deletion Syndrome Phenotypes

    NARCIS (Netherlands)

    Blok, Charlotte Snijders; Corsten-Janssen, Nicole; FitzPatrick, David R.; Romano, Corrado; Fichera, Marco; Vitello, Girolamo Aurelio; Willemsen, Marjolein H.; Schoots, Jeroen; Pfundt, Rolph; van Ravenswaaij-Arts, Conny M. A.; Hoefsloot, Lies; Kleefstra, Tjitske

    2014-01-01

    Microdeletions of the 5q11.2 region are rare; in literature only two patients with a deletion in this region have been reported so far. In this study, we describe four additional patients and further define this new 5q11.2 microdeletion syndrome. A comparison of the features observed in all six pati

  14. p53 protein expression independently predicts outcome in patients with lower-risk myelodysplastic syndromes with del(5q)

    Science.gov (United States)

    Saft, Leonie; Karimi, Mohsen; Ghaderi, Mehran; Matolcsy, András; Mufti, Ghulam J.; Kulasekararaj, Austin; Göhring, Gudrun; Giagounidis, Aristoteles; Selleslag, Dominik; Muus, Petra; Sanz, Guillermo; Mittelman, Moshe; Bowen, David; Porwit, Anna; Fu, Tommy; Backstrom, Jay; Fenaux, Pierre; MacBeth, Kyle J.; Hellström-Lindberg, Eva

    2014-01-01

    Del(5q) myelodysplastic syndromes defined by the International Prognostic Scoring System as low- or intermediate-1-risk (lower-risk) are considered to have an indolent course; however, recent data have identified a subgroup of these patients with more aggressive disease and poorer outcomes. Using deep sequencing technology, we previously demonstrated that 18% of patients with lower-risk del(5q) myelodysplastic syndromes carry TP53 mutated subclones rendering them at higher risk of progression. In this study, bone marrow biopsies from 85 patients treated with lenalidomide in the MDS-004 clinical trial were retrospectively assessed for p53 expression by immunohistochemistry in association with outcome. Strong p53 expression in ≥1% of bone marrow progenitor cells, observed in 35% (30 of 85) of patients, was significantly associated with higher acute myeloid leukemia risk (P=0.0006), shorter overall survival (P=0.0175), and a lower cytogenetic response rate (P=0.009), but not with achievement or duration of 26-week transfusion independence response. In a multivariate analysis, p53-positive immunohistochemistry was the strongest independent predictor of transformation to acute myeloid leukemia (P=0.0035). Pyrosequencing analysis of laser-microdissected cells with strong p53 expression confirmed the TP53 mutation, whereas cells with moderate expression predominantly had wild-type p53. This study validates p53 immunohistochemistry as a strong and clinically useful predictive tool in patients with lower-risk del(5q) myelodysplastic syndromes. This study was based on data from the MDS 004 trial (clinicaltrials.gov identifier: NCT00179621). PMID:24682512

  15. Cytogenetic follow-up by karyotyping and fluorescence in situ hybridization: implications for monitoring patients with myelodysplastic syndrome and deletion 5q treated with lenalidomide

    Science.gov (United States)

    Göhring, Gudrun; Giagounidis, Aristoteles; Büsche, Guntram; Hofmann, Winfried; Kreipe, Hans Heinrich; Fenaux, Pierre; Hellström-Lindberg, Eva; Schlegelberger, Brigitte

    2011-01-01

    In patients with low and intermediate risk myelodysplastic syndrome and deletion 5q (del(5q)) treated with lenalidomide, monitoring of cytogenetic response is mandatory, since patients without cytogenetic response have a significantly increased risk of progression. Therefore, we have reviewed cytogenetic data of 302 patients. Patients were analyzed by karyotyping and fluorescence in situ hybridization. In 85 patients, del(5q) was only detected by karyotyping. In 8 patients undergoing karyotypic evolution, the del(5q) and additional chromosomal aberrations were only detected by karyotyping. In 3 patients, del(5q) was only detected by fluorescence in situ hybridization, but not by karyotyping due to a low number of metaphases. Karyotyping was significantly more sensitive than fluorescence in situ hybridization in detecting the del(5q) clone. In conclusion, to optimize therapy control of myelodysplastic syndrome patients with del(5q) treated with lenalidomide and to identify cytogenetic non-response or progression as early as possible, fluorescence in situ hybridization alone is inadequate for evaluation. Karyotyping must be performed to optimally evaluate response. (clinicaltrials.gov identifier: NCT01099267 and NCT00179621) PMID:21109690

  16. Towards P2P XML Database Technology

    NARCIS (Netherlands)

    Y. Zhang (Ying)

    2007-01-01

    textabstractTo ease the development of data-intensive P2P applications, we envision a P2P XML Database Management System (P2P XDBMS) that acts as a database middle-ware, providing a uniform database abstraction on top of a dynamic set of distributed data sources. In this PhD work, we research which

  17. Variation in conserved non-coding sequences on chromosome 5q and susceptibility to asthma and atopy

    Directory of Open Access Journals (Sweden)

    Dubchak Inna

    2005-12-01

    Full Text Available Abstract Background Evolutionarily conserved sequences likely have biological function. Methods To determine whether variation in conserved sequences in non-coding DNA contributes to risk for human disease, we studied six conserved non-coding elements in the Th2 cytokine cluster on human chromosome 5q31 in a large Hutterite pedigree and in samples of outbred European American and African American asthma cases and controls. Results Among six conserved non-coding elements (>100 bp, >70% identity; human-mouse comparison, we identified one single nucleotide polymorphism (SNP in each of two conserved elements and six SNPs in the flanking regions of three conserved elements. We genotyped our samples for four of these SNPs and an additional three SNPs each in the IL13 and IL4 genes. While there was only modest evidence for association with single SNPs in the Hutterite and European American samples (P IL4 gene (P IL13 gene was strongly associated with total IgE (P = 0.00022 and allergic sensitization to mold allergens (P = 0.00076 in the Hutterites, and more modestly associated with sensitization to molds in the European Americans and African Americans (P Conclusion These results indicate that there is overall little variation in the conserved non-coding elements on 5q31, but variation in IL4 and IL13, including possibly one SNP in a conserved element, influence asthma and atopic phenotypes in diverse populations.

  18. A p53-dependent mechanism underlies macrocytic anemia in a mouse model of human 5q- syndrome.

    Science.gov (United States)

    Barlow, Jillian L; Drynan, Lesley F; Hewett, Duncan R; Holmes, Luke R; Lorenzo-Abalde, Silvia; Lane, Alison L; Jolin, Helen E; Pannell, Richard; Middleton, Angela J; Wong, See Heng; Warren, Alan J; Wainscoat, James S; Boultwood, Jacqueline; McKenzie, Andrew N J

    2010-01-01

    The identification of the genes associated with chromosomal translocation breakpoints has fundamentally changed understanding of the molecular basis of hematological malignancies. By contrast, the study of chromosomal deletions has been hampered by the large number of genes deleted and the complexity of their analysis. We report the generation of a mouse model for human 5q- syndrome using large-scale chromosomal engineering. Haploinsufficiency of the Cd74-Nid67 interval (containing Rps14, encoding the ribosomal protein S14) caused macrocytic anemia, prominent erythroid dysplasia and monolobulated megakaryocytes in the bone marrow. These effects were associated with defective bone marrow progenitor development, the appearance of bone marrow cells expressing high amounts of the tumor suppressor p53 and increased bone marrow cell apoptosis. Notably, intercrossing with p53-deficient mice completely rescued the progenitor cell defect, restoring common myeloid progenitor and megakaryocytic-erythroid progenitor, granulocyte-monocyte progenitor and hematopoietic stem cell bone marrow populations. This mouse model suggests that a p53-dependent mechanism underlies the pathophysiology of the 5q- syndrome.

  19. Identification of a haplotype block in the 5q31 cytokine gene cluster associated with the susceptibility to severe malaria

    Directory of Open Access Journals (Sweden)

    Tsuchiya Naoyuki

    2009-10-01

    Full Text Available Abstract Background It has been previously demonstrated that a single nucleotide polymorphism (SNP in the IL13 promoter region, IL13 -1055T>C (rs1800925, was associated with susceptibility to severe malaria in Thais. In the present study, fine association mapping for a cytokine gene cluster including IL4, IL5, and IL13 on chromosome 5q31 was conducted using the same malaria subjects to refine the region containing a primary variant or a haplotype susceptible to severe malaria. Methods A total of 82 SNPs spanning 522 kb of the 5q31 region were analysed in 368 patients with Plasmodium falciparum malaria (203 mild malaria and 165 severe malaria patients. Results Only rs1881457 located in the promoter region of IL13, which is in linkage disequilibrium with rs1800925 (r2 = 0.73, showed a significant association with severe malaria after adjusting for multiple testing (P = 0.046 by permutation test. This SNP was in a haplotype block spanning 97 kb (from rs2069812 to rs2240032. The detected haplotype block contained the RAD50 gene and the promoter of IL13, but not the other genes. Conclusion A haplotype block in which a primary polymorphism associated with severe malaria is likely to be encoded was identified in Thai malaria patients.

  20. Dramatic response in the dependency to transfusion after low doses of lenalidomide treatment in a 5q-syndrome patient: a case report.

    Science.gov (United States)

    Dogu, Mehmet Hilmi; Sari, Ismail; Hacioglu, Sibel; Keskin, Ali

    2014-01-01

    5q-syndrome is a special subgroup of myelodysplastic syndrome in terms of follow-up and treatment. Lenalidomide is an immunomodulatory drug that is frequently used in the treatment of multiple myeloma. Some clinical studies have shown that lenalidomide treatment is effective in 5q syndrome and significantly decreases the transfusion dependency in these patients. In this paper, we would like to share a dramatic response of lowered transfusion dependency after treatment with low-dose lenalidomide in a patient who received myelodysplastic syndrome diagnosis and isolated 5q anomaly in our clinic.

  1. Trisomy 2p: Analysis of unusual phenotypic findings

    Energy Technology Data Exchange (ETDEWEB)

    Lurie, I.W.; Ilyina, H.G.; Gurevich, D.B. [Belorussian Research Institute of Hereditary Disease, Minsk (Russian Federation)] [and others

    1995-01-16

    We present three probands with partial trisomies 2p21-23 due to ins(4;2)(q21;p21p23) pat, 2p23-pter due to t(2;4)(p23;q35)mat, and 2p21-pter due to t(2;11)(p21;q23.3)mat. More than 50 cases of partial trisomy 2p have been reviewed and some abnormalities, unusual for most other types of structural autosomal imbalance, have been found in patients with inherited forms of 2p trisomy and in their non-karyotyped sibs. Neural tube defects (anencephaly, occipital encephalocele, and spina bifida) were found in five probands and 4/6 affected non-karyotyped sibs. The only triplicated segment common to all was 2p24. Different forms of {open_quotes}broncho-pulmonary a/hypoplasia{close_quotes} (including two cases of lung agenesis) were described in four patients (overlapping triplicated segment was 2p21-p25). Three patients (with overlapping triplicated segment 2p23-p25) had diaphragmatic hernia. Abnormal rotation of the heart or L-transposition of large vessels (with or without visceral heterotaxia) was found in two infants (overlapping triplicated segment 2p23-p24). In two patients with common triplicated segment 2p22.3-p25, neuroblastoma has been described. The occurrence of all these defects may be explained either by the action of the same gene(s) mapped to 2p24 or by action of some independent factors located in different segments of the short arm. Although the latter hypothesis is much less probable, it can not be rejected at the present time. We propose the existence of a genetic system controlling surveillance of an abnormal embryo to explain the phenotypic differences between patients with the same imbalance within a family. In some {open_quotes}restrictive{close_quotes} combinations the abnormal embryos will die, although in {open_quotes}permissive{close_quotes} combinations they can survive. 47 refs., 2 figs., 3 tabs.

  2. $^3P_2$ Superfluids Are Topological

    CERN Document Server

    Mizushima, Takeshi

    2016-01-01

    We clarify the topology of the $^3P_2$ superfluidity which is expected to be realized in the cores of neutron stars and cubic odd-parity superconductors. The phase diagram includes the unitary uniaxial/biaxial nematic phases and nonunitary ferromagnetic and cyclic phases. We here show that the low-energy structures of all the phases are governed by different types of topologically protected gapless fermionic excitations: Surface Majorana fermions in nematic phases, single itinerant Majorana fermion in the ferromagnetic phase, and a quartet of itinerant Majorana fermions in the cyclic phase. Using the superfluid Fermi liquid theory, we also demonstrate that dihedral-two and -four biaxial nematic phases are thermodynamically favored in the weak coupling limit under a magnetic field. The mass acquisition of surface Majorana fermions in nematic phases is subject to symmetry.

  3. Early lenalidomide treatment for low and intermediate-1 International Prognostic Scoring System risk myelodysplastic syndromes with del(5q) before transfusion dependence.

    Science.gov (United States)

    Oliva, Esther N; Lauseker, Michael; Aloe Spiriti, Maria Antonietta; Poloni, Antonella; Cortelezzi, Agostino; Palumbo, Giuseppe A; Balleari, Enrico; Sanpaolo, Grazia; Volpe, Antonio; Ricco, Alessandra; Ronco, Francesca; Alati, Caterina; D'Errigo, Maria Grazia; Santacaterina, Irene; Kündgen, Andrea; Germing, Ulrich; Latagliata, Roberto

    2015-12-01

    Lenalidomide is approved for the treatment of transfusion-dependent (TD) del(5q) myelodysplastic syndromes (MDS). However, few data are available in patients with transfusion-independent (TI) del(5q) MDS. In the first, observational, part of this 2-part study, we assessed the impact of transfusion dependence on overall survival (OS) and non-leukemic death in untreated del(5q) MDS patients who were TD (n = 136), TI with hemoglobin (Hb) ≥10 mg/dL (n = 88), or TI with Hb lenalidomide (10 mg/day) in 12 patients with TI del(5q) MDS and Hb lenalidomide (+12.5; P = 0.020). Evaluable TI patients experienced early increases in Hb levels, and all attained an erythroid response. Our findings suggest that TI patients with moderate anemia may benefit from early treatment with lenalidomide.

  4. High level of full-length cereblon mRNA in lower risk myelodysplastic syndrome with isolated 5q deletion is implicated in the efficacy of lenalidomide.

    Science.gov (United States)

    Jonasova, Anna; Bokorova, Radka; Polak, Jaroslav; Vostry, Martin; Kostecka, Arnost; Hajkova, Hana; Neuwirtova, Radana; Siskova, Magda; Sponerova, Dana; Cermak, Jaroslav; Mikulenkova, Dana; Cervinek, Libor; Brezinova, Jana; Michalova, Kyra; Fuchs, Ota

    2015-07-01

    Downregulation of cereblon (CRBN) gene expression is associated with resistance to the immunomodulatory drug lenalidomide and poor survival outcomes in multiple myeloma (MM) patients. However, the importance of CRBN gene expression in patients with myelodysplastic syndrome (MDS) and its impact on lenalidomide therapy are not clear. In this study, we evaluate cereblon expression in mononuclear cells isolated from bone marrow [23 lower risk MDS patients with isolated 5q deletion (5q-), 37 lower risk MDS patients with chromosome 5 without the deletion of long arms (non-5q-), and 24 healthy controls] and from peripheral blood (38 patients with 5q-, 52 non-5q- patients and 25 healthy controls) to gain insight into, firstly, the role of cereblon in lower risk MDS patients with or without 5q deletion and, secondly, into the mechanisms of lenalidomide action. Patients with 5q- lower risk MDS have the highest levels of CRBN mRNA in comparison with both lower risk MDS without the deletion of long arms of chromosome 5 and healthy controls. CRBN gene expression was measured using the quantitative TaqMan real-time PCR. High levels of CRBN mRNA were detected in all lenalidomide responders during the course of therapy. A significant decrease of the CRBN mRNA level during lenalidomide treatment is associated with loss of response to treatment and disease progression. These results suggest that, similar to the treatment of MM, high levels of full-length CRBN mRNA in lower risk 5q- patients are necessary for the efficacy of lenalidomide.

  5. P2P overlay over Simctl

    OpenAIRE

    Sicart López, Gerard Carles

    2016-01-01

    This project intends to find and implement a p2p overlay over the simctl platform. Simctl is used both for teaching and research in the network engineering department. P2P systems became an interesting area since early 2000. Researchers conducted a large amount of research in some challenging areas and, to check their experiments, several implementations and simulators were created. Over time, Internet has evolved and P2P has been widely used for file sharing, but the main structured P2P o...

  6. Lenalidomide as a disease-modifying agent in patients with del(5q) myelodysplastic syndromes: linking mechanism of action to clinical outcomes.

    Science.gov (United States)

    Giagounidis, Aristoteles; Mufti, Ghulam J; Fenaux, Pierre; Germing, Ulrich; List, Alan; MacBeth, Kyle J

    2014-01-01

    Deletion of the long arm of chromosome 5, del(5q), is the most prevalent cytogenetic abnormality in patients with myelodysplastic syndromes (MDS). In isolation, it is traditionally associated with favorable prognosis compared with other subtypes of MDS. However, owing to the inherent heterogeneity of the disease, prognosis for patients with del(5q) MDS is highly variable depending on the presence of factors such as additional chromosomal abnormalities, >5 % blasts in the bone marrow (BM), or transfusion dependence. Over recent years, the immunomodulatory drug lenalidomide has demonstrated remarkable efficacy in patients with del(5q) MDS. Advances in the understanding of the pathogenesis of the disease have suggested that lenalidomide targets aberrant signaling pathways caused by haplosufficiency of specific genes in a commonly deleted region on chromosome 5 (e.g., SPARC, RPS14, Cdc25C, and PP2A). As a result, the agent specifically targets del(5q) clones while also promoting erythropoiesis and repopulation of the bone marrow in normal cells. This review discusses recent developments in the understanding of the mechanism of action of lenalidomide, and how this underlies favorable outcomes in patients with del(5q) MDS. In addition, we discuss how improved understanding of the mechanism of disease will facilitate clinicians' ability to predict/monitor response and identify patients at risk of relapse.

  7. Coinage metal complexes supported by a "PN(3)P" scaffold.

    Science.gov (United States)

    Rao, Gyandshwar Kumar; Gorelsky, Serge I; Korobkov, Ilia; Richeson, Darrin

    2015-11-28

    A series of monovalent group 11 complexes, [2,6-{Ph2PNMe}2(NC5H3)]CuBr 1, [2,6-{Ph2PNMe}2(NC5H3)]CuOTf 2, [2,6-{Ph2PNMe}2(NC5H3)]AgOTf 3, and [2,6-{Ph2PNMe}2(NC5H3)](AuCl)24, supported by a neutral PN(3)P ligand have been synthesized and characterized by multinuclear NMR and single crystal X-ray diffraction studies. The variation of the coordination properties were analyzed and electronic structure calculations have been carried out to provide insight on the bonding details in these complexes. The Cu(I) complexes displayed an unusual coordination geometry with a tridentate pincer ligand and an overall four coordinate trigonal pyramidal geometry. In contrast the Ag(I) analogue displayed a bidentate κ(2)-P,P' ligation leaving the pyridyl-N atom uncoordinated and yielding a pyramidalized trigonal planar geometry around Ag. The bimetallic Au(I) complex completed the series and displayed a monodentate P-bonded ligand and a linear coordination geometry.

  8. Characterization of P2P Systems

    Science.gov (United States)

    Stutzbach, Daniel; Rejaie, Reza

    The combination of large scale and geographically distributed nature of P2P system has led to their significant impact on the Internet. It is essential to characterize deployed P2P system for at least three reasons: (1) Accurately assessing their impact on the Internet, (2) identifying any performance bottleneck as well as any opportunity for performance improvement, (3) understanding user-driven dynamics in P2P systems. To characterize a P2P system, one needs to accurately capture snapshots of the resulting P2P overlay. This is challenging because the overlay is often large and dynamic. While the overlay is discovered by a crawler, it is changing which leads to a distorted view of the system. Capturing unbiased view of the traffic in the overlay is equally challenging because it is difficult to show that the captured behavior represent the observed behavior by all peers. In this chapter, we describe some of the fundamental problems in empirical characterization of widely deployed P2P systems. We present several examples to illustrate the effect of ad-hoc measurement/data collection on the resulting analysis/characterization. We then present two sampling techniques as a powerful approach to capture unbiased view of peer properties in a scalable fashion.

  9. Extending an Afrikaans pronunciation dictionary using Dutch resources and P2P/GP2P

    CSIR Research Space (South Africa)

    Loots, L

    2010-11-01

    Full Text Available . This is compared to the more common approach of extending the Afrikaans dictionary by means of graphemeto-phoneme (G2P) conversion. The results indicate that the Afrikaans pronunciations obtained by P2P and GP2P from the Dutch dictionary are more accurate than...

  10. A novel unbalanced de novo translocation der(5t(4;5(q26;q21.1 in adult T-cell precursor lymphoblastic leukemia

    Directory of Open Access Journals (Sweden)

    Kjeldsen Eigil

    2012-05-01

    Full Text Available Abstract We here describe a novel unbalanced de novo translocation der(5t(4;5(q26;q21.1 in a 39-year-old male diagnosed with acute T-cell lymphoblastic leukemia. Bone marrow (BM was massively infiltrated with 85 % highly proliferative polymorphic T-cell precursors. Immunologically, the malignant cells stained positive for CD7, CD34, intracytoplasmic CD3+, TdT + and negative for CD3 and CD5. G-banded chromosome analysis of BM cells showed the normal karyotype 46,XY[25] whereas BAC-based aCGH analysis revealed partial gain of 4q and partial loss of 5q. Multicolor karyotyping confirmed the presence of an unbalanced der(5t(4;5 as the sole structural abnormality. Subsequent high-resolution oligonucleotide-based aCGH analysis showed that the der(5t(4;5(q26;q21.1 resulted in partial trisomy of 4q26qter (117,719,015-190,613,014 and partial monosomy of 5q21.1qter (100,425,442-180,857,866 and that there was no indication of any gene disruptions resulting from the breakages. Interphase FISH analysis using BAC-based specific probes for 4q26 and 5q21.1 confirmed the breakpoints and revealed approximately 80 % abnormal cells accordingly. At 4q26 the MIR1973 gene is located centromeric to the breakpoint in the copy number neutral region and the TRAM1L1 gene is located within the gained region. At 5q21.1 the genes ST8SIA4 and MIR548p are located centromeric to the breakpoint and no known genes up to approximately 1 Mb telomeric to the breakpoint in the copy number loss region. Interestingly, only the gene ST8SIA4 at 5q21.1 have been implicated in T-cell regulation as it encodes one of the key enzymes for polysialysation of surface proteins on dendritic cells which are important regulators for T-cell proliferation. The der(5t(4;5 is thought to play a crucial role in the pathogenesis of acute T-ALL due to either gain of 4q, the loss of 5q, or deregulation of genes in proximity to the breakpoints.

  11. The dielectronic satellites to the 2s-3p Ne-like krypton resonance lines

    Energy Technology Data Exchange (ETDEWEB)

    Khakhalin, S.Ya. (MISDC, NPO ' ' VNIIFTRI' ' , Mendeleevo (Russian Federation)); Dyakin, V.M. (MISDC, NPO ' ' VNIIFTRI' ' , Mendeleevo (Russian Federation)); Faenov, A.Ya. (MISDC, NPO ' ' VNIIFTRI' ' , Mendeleevo (Russian Federation)); Fiedorowicz, H. (Inst. of Optoelectronics, Warsaw (Poland)); Bartnik, A. (Inst. of Optoelectronics, Warsaw (Poland)); Parys, P. (Inst. of Plasma Physics and Laser Microfusion, Warsaw (Poland)); Nilsen, J. (Lawrence Livermore National Lab., Livermore, CA (United States)); Osterheld, A. (Lawrence Livermore National Lab., Livermore, CA (United States))

    1994-08-01

    We present an analysis of dielectronic satellite spectra of 2p[sup 6]-2s2p[sup 6]3p Ne-like krypton resonance lines. The satellite structure was registered with high (better than [lambda]/[Delta][lambda] > 3500) spectral resolution in the emission of a laser irradiated gas puff target. We perform an unambiguous identification of satellite lines caused by radiative transitions from autoionizing states of sodium-like krypton ions. A total of about 20 spectral features are identified, most of them for the first time. Very good agreement between the satellite structure calculations and experimental emission spectra is obtained. (orig.).

  12. Fluorescence in situ hybridization for del(5q) in myelodysplasia/acute myeloid leukemia: comparison of EGR1 vs. CSF1R probes and diagnostic yield over metaphase cytogenetics alone.

    Science.gov (United States)

    Sun, Yang; Cook, James R

    2010-03-01

    To determine the clinical utility of FISH for del(5q) in MDS/AML, we first compared FISH for 5q31 (EGR1) and 5q33 (CSF1R) in 51 myeloid neoplasms containing del(5q) by metaphase cytogenetics. Next, EGR1 FISH was compared to metaphase cytogenetics alone in 269 cases of known or suspected MDS/AML. These studies show that while metaphase cytogenetics alone can detect del(5q) in most cases, FISH is particularly useful in cases with suboptimal growth. EGR1 FISH detects del(5q) in a broad variety of myeloid neoplasms, including at least most cases of 5q- syndrome, while studies for CSF1R add little to the diagnostic yield.

  13. Association of chromosome 5q21.3 polymorphisms with the exploratory eye movement dysfunction in schizophrenia

    Science.gov (United States)

    Ma, Yuanlin; Li, Jun; Yu, Hao; Wang, Lifang; Lu, Tianlan; Pan, Chao; Han, Yonghua; Zhang, Dai; Yue, Weihua

    2015-01-01

    Schizophrenia patients show abnormalities in many eye movement tasks. Among them, exploratory eye movements (EEM) dysfunction seems to be specific to schizophrenia. However the mechanism of EEM disturbances in schizophrenia patients remains elusive. We investigate the relationship between EEM and single nucleotide polymorphisms (SNPs) or genes to identify susceptibility loci for EEM in schizophrenia. We firstly performed EEM test, then performed a genome-wide association study (GWAS) and gene-based association study of EEM in 128 individuals with schizophrenia and 143 healthy control subjects. Comparing to healthy controls, schizophrenia patients show significant decrease in NEF (22.99 ± 3.96 vs. 26.02 ± 5.72, P <0.001), TESL (368.78 ± 123.57 vs. 603.12 ± 178.63, P <0.001), MESL (16.86 ± 5.27 vs. 24.42 ± 6.46, P <0.001), RSS (8.22 ± 1.56 vs. 10.92 ± 1.09, P <0.001), and CSS (5.06 ± 0.97 vs. 6.64 ± 0.87, P <0.001). Five SNPs of the MAN2A1, at 5q21.3, were associated with EEM abnormalities (deceased CSS) and satisfied the criteria of GWAS significance threshold. One is localized near 5’-UTR (rs17450784) and four are in intron (rs1438663, rs17162094, rs6877440 and rs10067856) of the gene. Our findings suggest that the identified loci may control the schizophrenia-related quantitative EEM trait. And the identified gene, associated with the EEM phenotype, may lead to new insights into the etiology of schizophrenia. PMID:26242244

  14. Spectra and oscillator strengths of 3p63d9-3p53d10 and 3p63d9-3p63d84p transitions for cobalt-like Sn23+ ion

    Institute of Scientific and Technical Information of China (English)

    Chen Ming-Lun; Yu Xiao-Guang

    2009-01-01

    This paper calculates the spectra and oscillator strengths for highly ionized cobalt-like Sn23+ ions 3p63d9-3p53d10,3p63d9 - 3p63d84p transitions by using a multi-configuration self-consistent field method program together with the proposed fitting formula. The calculations have a good agreement with observations.

  15. P2P Techniques for Decentralized Applications

    CERN Document Server

    Pacitti, Esther

    2012-01-01

    As an alternative to traditional client-server systems, Peer-to-Peer (P2P) systems provide major advantages in terms of scalability, autonomy and dynamic behavior of peers, and decentralization of control. Thus, they are well suited for large-scale data sharing in distributed environments. Most of the existing P2P approaches for data sharing rely on either structured networks (e.g., DHTs) for efficient indexing, or unstructured networks for ease of deployment, or some combination. However, these approaches have some limitations, such as lack of freedom for data placement in DHTs, and high late

  16. Configuration interaction calculations of positron binding to Be({sup 3}P )

    Energy Technology Data Exchange (ETDEWEB)

    Bromley, M.W.J. [Department of Physics, San Diego State University, San Diego, CA 92182 (United States)]. E-mail: mbromley@physics.sdsu.edu; Mitroy, J. [Faculty of Technology, Charles Darwin University, Darwin, NT 0909 (Australia)]. E-mail: jxm107@rsphysse.anu.edu.au

    2006-06-15

    The configuration interaction method is applied to investigate the possibility of positron binding to the metastable beryllium (1s{sup 2}2s2p {sup 3}P ) state. The largest calculation obtained an estimated energy that was unstable by 0.00014 Hartree with respect to the Ps + Be{sup +}(2s) lowest dissociation channel. It is likely that positron binding to parent states with non-zero angular momentum is inhibited by centrifugal barriers.

  17. Outcomes in RBC transfusion-dependent patients with Low-/Intermediate-1-risk myelodysplastic syndromes with isolated deletion 5q treated with lenalidomide: a subset analysis from the MDS-004 study

    Science.gov (United States)

    Giagounidis, Aristoteles; Mufti, Ghulam J; Mittelman, Moshe; Sanz, Guillermo; Platzbecker, Uwe; Muus, Petra; Selleslag, Dominik; Beyne-Rauzy, Odile; te Boekhorst, Peter; del Cañizo, Consuelo; Guerci-Bresler, Agnès; Nilsson, Lars; Lübbert, Michael; Quesnel, Bruno; Ganser, Arnold; Bowen, David; Schlegelberger, Brigitte; Göhring, Gudrun; Fu, Tommy; Benettaib, Bouchra; Hellström-Lindberg, Eva; Fenaux, Pierre

    2014-01-01

    Objective A subset analysis of the randomised, phase 3, MDS-004 study to evaluate outcomes in patients with International Prognostic Scoring System (IPSS)-defined Low-/Intermediate (Int)-1-risk myelodysplastic syndromes (MDS) with isolated del(5q). Methods Patients received lenalidomide 10 mg/d (days 1–21; n = 47) or 5 mg/d (days 1–28; n = 43) on 28-d cycles or placebo (n = 45). From the placebo and lenalidomide 5 mg groups, 84% and 58% of patients, respectively, crossed over to lenalidomide 5 or 10 mg at 16 wk, respectively. Results Rates of red blood cell-transfusion independence (RBC-TI) ≥182 d were higher in the lenalidomide 10 mg (57.4%; P < 0.0001) and 5 mg (37.2%; P = 0.0001) groups vs. placebo (2.2%). Cytogenetic response rates (major + minor responses) were 56.8% (P < 0.0001), 23.1% (P = 0.0299) and 0%, respectively. Two-year cumulative risk of acute myeloid leukaemia progression was 12.6%, 17.4% and 16.7% in the lenalidomide 10 mg, 5 mg, and placebo groups, respectively. In a 6-month landmark analysis, overall survival was longer in lenalidomide-treated patients with RBC-TI ≥182 d vs. non-responders (P = 0.0072). The most common grade 3–4 adverse event was myelosuppression. Conclusions These data support the clinical benefits and acceptable safety profile of lenalidomide in transfusion-dependent patients with IPSS-defined Low-/Int-1-risk MDS with isolated del(5q). PMID:24813620

  18. A randomized phase 3 study of lenalidomide versus placebo in RBC transfusion-dependent patients with Low-/Intermediate-1-risk myelodysplastic syndromes with del5q

    NARCIS (Netherlands)

    Fenaux, P.; Giagounidis, A.; Selleslag, D.; Beyne-Rauzy, O.; Mufti, G.; Mittelman, M.; Muus, P.; Boekhorst, P. Te; Sanz, G.; Del Canizo, C.; Guerci-Bresler, A.; Nilsson, L.; Platzbecker, U.; Lubbert, M.; Quesnel, B.; Cazzola, M.; Ganser, A.; Bowen, D.; Schlegelberger, B.; Aul, C.; Knight, R.; Francis, J.; Fu, T.; Hellstrom-Lindberg, E.

    2011-01-01

    This phase 3, randomized, double-blind study assessed the efficacy and safety of lenalidomide in 205 red blood cell (RBC) transfusion-dependent patients with International Prognostic Scoring System Low-/Intermediate-1-risk del5q31 myelodysplastic syndromes. Patients received lenalidomide 10 mg/day o

  19. Switch in FGFR3 and -4 expression profile during human renal development may account for transient hypercalcemia in patients with Sotos syndrome due to 5q35 microdeletions

    NARCIS (Netherlands)

    Mutsaers, Henricus A M; Levtchenko, Elena N; Martinerie, Laetitia; Pertijs, Jeanne C L M; Allegaert, Karel; Devriendt, Koenraad; Masereeuw, Roos; Monnens, Leo A H; Lombès, Marc

    2014-01-01

    CONTEXT: Sotos syndrome is a rare genetic disorder with a distinct phenotypic spectrum including overgrowth and learning difficulties. Here we describe a new case of Sotos syndrome with a 5q35 microdeletion, affecting the fibroblast growth factor receptor 4 (FGFR4) gene, presenting with infantile hy

  20. Delayed globin synthesis leads to excess heme and the macrocytic anemia of Diamond Blackfan anemia and del(5q) myelodysplastic syndrome.

    Science.gov (United States)

    Yang, Zhantao; Keel, Siobán B; Shimamura, Akiko; Liu, Li; Gerds, Aaron T; Li, Henry Y; Wood, Brent L; Scott, Bart L; Abkowitz, Janis L

    2016-05-11

    Diamond Blackfan anemia (DBA) and myelodysplastic syndrome (MDS) with isolated del(5q) are severe macrocytic anemias; although both are associated with impaired ribosome assembly, why the anemia occurs is not known. We cultured marrow cells from DBA (n = 3) and del(5q) MDS (n = 6) patients and determined how heme (a toxic chemical) and globin (a protein) are coordinated. We show that globin translation initiates slowly, whereas heme synthesis proceeds normally. This results in insufficient globin protein, excess heme and excess reactive oxygen species in early erythroid precursors, and CFU-E (colony-forming unit-erythroid)/proerythroblast cell death. The cells that can more rapidly and effectively export heme or can slow heme synthesis preferentially survive and appropriately mature. Consistent with these observations, treatment with 10 μM succinylacetone, a specific inhibitor of heme synthesis, improved the erythroid cell output of DBA and del(5q) MDS marrow cultures by 68 to 95% (P = 0.03 to 0.05), whereas the erythroid cell output of concurrent control marrow cultures decreased by 4 to 13%. Our studies demonstrate that erythropoiesis fails when heme exceeds globin. Our data further suggest that therapies that decrease heme synthesis (or facilitate heme export) could improve the red blood cell production of persons with DBA, del(5q) MDS, and perhaps other macrocytic anemias.

  1. Lenalidomide for the Treatment of Low- or Intermediate-1-Risk Myelodysplastic Syndromes Associated with Deletion 5q Cytogenetic Abnormality: An Evidence Review of the NICE Submission from Celgene

    NARCIS (Netherlands)

    H.M. Sluimer-Blommestein (Hedwig); N. Armstrong (Nigel); S. Ryder; S. Deshpande (Sohan); G. Worthy (Gill); C. Noake; R. Riemsma; J. Kleijnen (Jos); J.L. Severens (Hans); M.J. Al (Maiwenn)

    2016-01-01

    textabstractThe National Institute for Health and Care Excellence (NICE) invited the manufacturer of lenalidomide (Celgene) to submit evidence of the clinical and cost effectiveness of the drug for treating adults with myelodysplastic syndromes (MDS) associated with deletion 5q cytogenetic abnormali

  2. Multivariate time-dependent comparison of the impact of lenalidomide in lower-risk myelodysplastic syndromes with chromosome 5q deletion.

    Science.gov (United States)

    Sánchez-García, Joaquín; Del Cañizo, Consuelo; Lorenzo, Ignacio; Nomdedeu, Benet; Luño, Elisa; de Paz, Raquel; Xicoy, Blanca; Valcárcel, David; Brunet, Salut; Marco-Betes, Victor; García-Pintos, Marta; Osorio, Santiago; Tormo, Mar; Bailén, Alicia; Cerveró, Carlos; Ramos, Fernando; Diez-Campelo, María; Such, Esperanza; Arrizabalaga, Beatriz; Azaceta, Gemma; Bargay, Joan; Arilla, María J; Falantes, José; Serrano-López, Josefina; Sanz, Guillermo F

    2014-07-01

    The impact of lenalidomide treatment on long-term outcomes of patients with lower risk myelodysplastic syndromes (MDS) and chromosome 5q deletion (del(5q)) is unclear. This study used time-dependent multivariate methodology to analyse the influence of lenalidomide therapy on overall survival (OS) and acute myeloblastic leukaemia (AML) progression in 215 patients with International Prognostic Scoring System (IPSS) low or intermediate-1 risk and del(5q). There were significant differences in several relevant characteristics at presentation between patients receiving (n = 86) or not receiving lenalidomide (n = 129). The 5-year time-dependent probabilities of OS and progression to AML were 62% and 31% for patients receiving lenalidomide and 42% and 25% for patients not receiving lenalidomide; differences were not statistically significant in multivariate analysis that included all variables independently associated with those outcomes (OS, P = 0·45; risk of AML, P = 0·31, respectively). Achievement of RBC transfusion independency (P = 0·069) or cytogenetic response (P = 0·021) after lenalidomide was associated with longer OS in multivariate analysis. These data clearly show that response to lenalidomide results in a substantial clinical benefit in lower risk MDS patients with del(5q). Lenalidomide treatment does not appear to increase AML risk in this population of patients.

  3. 31 CFR 30.5 - Q-5: How does a TARP recipient comply with the requirements under § 30.4 (Q-4) of this part that...

    Science.gov (United States)

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Q-5: How does a TARP recipient comply... recipient? 30.5 Section 30.5 Money and Finance: Treasury Office of the Secretary of the Treasury TARP STANDARDS FOR COMPENSATION AND CORPORATE GOVERNANCE § 30.5 Q-5: How does a TARP recipient comply with the...

  4. Oscillator strength for 3s{sup 2}3p{sup 5}-3s3p{sup 6} in Cl-like ions

    Energy Technology Data Exchange (ETDEWEB)

    Berrington, K.A.; Waldock, J.A. [School of Science and Mathematics, Sheffield Hallam University, Sheffield (United Kingdom); Pelan, J.C [Gatsby Computational Neuroscience Unit, University College, London (United Kingdom)

    2001-07-14

    Recent experiments have revealed discrepancies between calculated and experimental lifetimes in the lowest excited level of some chlorine-like ions. New ab initio oscillator strengths using the Breit-Pauli R-matrix method are provided for the 3s{sup 2}3p{sup 5} {sup 2}P{sup o}{sub 3/2,1/2}-3s3p{sup 6} {sup 2}S{sup e}{sub 1/2} transition in Cl-like Ar II through to Cu XIII. Our calculated lifetimes for these twelve ions are respectively 5.507, 2.077, 1.177, 0.784, 0.569, 0.438, 0.350, 0.287, 0.241, 0.206, 0.178 and 0.156 ns, and are more consistent with experiments than other calculations. (author). Letter-to-the-editor.

  5. Lenalidomide: a review of its use in patients with transfusion-dependent anaemia due to low- or intermediate-1-risk myelodysplastic syndrome associated with 5q chromosome deletion.

    Science.gov (United States)

    Syed, Yahiya Y; Scott, Lesley J

    2013-07-01

    Lenalidomide (Revlimid(®)), a thalidomide analogue, is an orally administered second generation immunomodulator with anti-angiogenic, antineoplastic, anti-inflammatory and pro-erythropoietic properties. It is approved for the treatment of patients with transfusion-dependent anaemia due to International Prognostic Scoring System low- or intermediate-1-risk myelodysplastic syndrome (MDS) associated with either chromosome 5q deletion [del(5q)] with or without additional cytogenetic abnormalities (US, Japan and Switzerland etc.), or with an isolated del(5q) cytogenetic abnormality when other therapeutic options are insufficient or inadequate (EU) [featured indication]. In a randomized, double-blind, multicentre, registrational trial (MDS-004; n = 205) in this patient population, a significantly higher proportion of lenalidomide recipients than placebo recipients achieved red blood cell transfusion independence for ≥26 consecutive weeks (primary endpoint for efficacy) and cytogenetic responses. The erythroid response to lenalidomide was accompanied by an increase in the haemoglobin levels. These efficacy outcomes are generally consistent with those seen in an earlier noncomparative registrational trial (MDS-003; n = 148). In MDS-004, lenalidomide also significantly improved health-related quality of life compared with placebo at 12 weeks. Retrospective analyses that compared outcomes between lenalidomide-treated patients with low- or intermediate-1-risk del(5q) MDS and multicentre registry cohorts showed that lenalidomide treatment did not appear to increase the risk of progression to acute myeloid leukaemia. Lenalidomide had a manageable safety profile in the registrational trials, with ≤20 % of patients discontinuing treatment because of adverse events. The most common adverse events (incidence ≥20 %) occurring in lenalidomide recipients were thrombocytopenia and neutropenia, which were generally managed by dosage reductions and/or interruptions, and

  6. Data Sharing in P2P Systems

    Science.gov (United States)

    Hayek, Rabab; Raschia, Guillaume; Valduriez, Patrick; Mouaddib, Noureddine

    In this chapter, we survey P2P data sharing systems. All along, we focus on the evolution from simple file-sharing systems, with limited functionalities, to Peer Data Management Systems (PDMS) that support advanced applications with more sophisticated data management techniques. Advanced P2P applications are dealing with semantically rich data (e.g., XML documents, relational tables), using a high-level SQL-like query language. We start our survey with an overview over the existing P2P network architectures, and the associated routing protocols. Then, we discuss data indexing techniques based on their distribution degree and the semantics they can capture from the underlying data. We also discuss schema management techniques which allow integrating heterogeneous data. We conclude by discussing the techniques proposed for processing complex queries (e.g., range and join queries). Complex query facilities are necessary for advanced applications which require a high level of search expressiveness. This last part shows the lack of querying techniques that allow for an approximate query answering.

  7. XQ2P: Efficient XQuery P2P Time Series Processing

    CERN Document Server

    Butnaru, Bogdan; Gardarin, Georges; Yeh, Laurent

    2010-01-01

    In this demonstration, we propose a model for the management of XML time series (TS), using the new XQuery 1.1 window operator. We argue that centralized computation is slow, and demonstrate XQ2P, our prototype of efficient XQuery P2P TS computation in the context of financial analysis of large data sets (>1M values).

  8. Quark structure of the X(3872) and χb(3P) resonances

    Science.gov (United States)

    Ferretti, J.; Galatà, G.; Santopinto, E.

    2014-09-01

    We discuss the nature of the χb(3P) and X(3872) mesons. Are the χb(3P)'s standard bb¯ mesons or bb¯ states with a significative continuum component? Is the X(3872) a cc¯ state with continuum coupling effects or a meson-meson molecule? To do that, we compare quark model and unquenched quark model results for the mass barycenter and splittings of the χb(3P) multiplet. Future and more precise experimental results will discriminate between the two interpretations. In the case of the X(3872), we interpret it as a cc¯ core plus higher Fock components due to the coupling to the meson-meson continuum, and thus we think that it is compatible with the meson χc1(2P), with JPC=1++. The JPC=1++ quantum numbers are in agreement with the experimental results found by the LHCb collaboration. In our view, the X(3872)'s mass is lower than the quark model's predictions because of self-energy shifts. We also provide an estimation of the open charm/bottom strong decay modes of the X(3872) and χb(3P) mesons, such as X(3872)→DD¯* and χb2(3P)→BB¯, and radiative transitions.

  9. Gene Expression Meta-Analysis identifies Cytokine Pathways and 5q Aberrations involved in Metastasis of ERBB2 Amplified and Basal Breast Cancer

    DEFF Research Database (Denmark)

    Thomassen, Mads; Tan, Qihua; Burton, Mark

    2013-01-01

    Background: Breast tumors have been described by molecular subtypes characterized by pervasively different gene expression profiles. The subtypes are associated with different clinical parameters and origin of precursor cells. However, the biological pathways and chromosomal aberrations that differ...... the subgroups impact metastasis. Results: We have scrutinized publicly available gene expression datasets and identified molecular subtypes in 1,394 breast tumors with outcome data. By analysis of chromosomal regions and pathways using “Gene set enrichment analysis” followed by a meta-analysis, we identified...... show that high expression of 5q14 genes and low levels of TNFR2 pathway genes were associated with poor survival in basal-like cancers. Furthermore, low expression of 5q33 genes and interleukin-12 pathway genes were associated with poor outcome exclusively in ERBB2-like tumors. Conclusion...

  10. 5q14.3 deletion neurocutaneous syndrome: Contiguous gene syndrome caused by simultaneous deletion of RASA1 and MEF2C: A progressive disease.

    Science.gov (United States)

    Ilari, Rita; Agosta, Guillermo; Bacino, Carlos

    2016-03-01

    We report the case of a young girl who was presented with complex clinical symptoms caused by the deletion of contiguous genes: RASA1 and MEF2C, located on chromosome 5q14.3. Specifically, the diagnosis of her skin disorder and vascular malformations involving central nervous system is consistent with a RASopathy. The child's neurological manifestations are observed in most patients suffering from 5q14.3 by deletion or mutation of the MEF2C gene. A review of the literature allowed us to conclude that the contiguous deletion of genes RASA1 and MEF2C fulfills the criteria for the diagnosis of a Neurocutaneous syndrome as proposed by Carr et al. [2011]. We also assessed the penetrance of RASA1 and clinical manifestations of MEF2C according to the type of deletion. This child described presents the complete symptomatology of both deleted genes. We would also like to highlight the progression of the disorder.

  11. CCQE, 2p2h excitations and \

    CERN Document Server

    Nieves, J; Sánchez, F; Vacas, M J Vicente

    2013-01-01

    We analyze the MiniBooNE muon neutrino CCQE-like d\\sigma/dT_\\mu/dcos\\theta_\\mu data using a theoretical model that, among other nuclear effects, includes RPA correlations and 2p2h (multinucleon) mechanisms. These corrections turn out to be essential for the description of the data. We find that MiniBooNE CCQE-like data are fully compatible with former determinations of the nucleon axial mass M_A ~ 1.05 GeV. This is in sharp contrast with several previous analysis where anomalously large values of M_A ~ 1.4 GeV have been suggested. We also show that because of the the multinucleon mechanism effects, the algorithm used to reconstruct the neutrino energy is not adequate when dealing with quasielastic-like events. Finally, we analyze the MiniBooNE unfolded cross section, and show that it exhibits an excess (deficit) of low (high) energy neutrinos, which is an artifact of the unfolding process that ignores 2p2h mechanisms.

  12. Association between Alcoholism and the Genetic Polymorphisms of the GABAA Receptor Genes on Chromosome 5q33-34 in Korean Population

    OpenAIRE

    Park, Chul-Soo; Park, So-Young; Lee, Chul-Soon; Sohn, Jin-Wook; Hahn, Gyu-Hee; Kim, Bong-Jo

    2006-01-01

    Family, twin, and adoption studies have demonstrated that genes play an important role in the development of alcoholism. We investigated the association between alcoholism and the genetic polymorphisms of the GABAA receptor genes on chromosome 5q33-34 in Korean population. The genotype of the GABAA receptor gene polymorphisms were determined by performing polymerase chain reaction genotyping for 172 normal controls and 162 male alcoholics who are hospitalized in alcoholism treatment institute...

  13. A mitotic recombination map proximal to the APC locus on chromosome 5q and assessment of influences on colorectal cancer risk

    Directory of Open Access Journals (Sweden)

    Clark Susan

    2009-06-01

    Full Text Available Abstract Background Mitotic recombination is important for inactivating tumour suppressor genes by copy-neutral loss of heterozygosity (LOH. Although meiotic recombination maps are plentiful, little is known about mitotic recombination. The APC gene (chr5q21 is mutated in most colorectal tumours and its usual mode of LOH is mitotic recombination. Methods We mapped mitotic recombination boundaries ("breakpoints" between the centromere (~50 Mb and APC (~112 Mb in early colorectal tumours. Results Breakpoints were non-random, with the highest frequency between 65 Mb and 75 Mb, close to a low copy number repeat region (68–71 Mb. There were, surprisingly, few breakpoints close to APC, contrary to expectations were there constraints on tumorigenesis caused by uncovering recessive lethal alleles or if mitotic recombination were mechanistically favoured by a longer residual chromosome arm. The locations of mitotic and meiotic recombination breakpoints were correlated, suggesting that the two types of recombination are influenced by similar processes, whether mutational or selective in origin. Breakpoints were also associated with higher local G+C content. The recombination and gain/deletion breakpoint maps on 5q were not, however, associated, perhaps owing to selective constraints on APC dosage in early colorectal tumours. Since polymorphisms within the region of frequent mitotic recombination on 5q might influence the frequency of LOH, we tested the 68–71 Mb low copy number repeat and nearby tagSNPs, but no associations with colorectal cancer risk were found. Conclusion LOH on 5q is non-random, but local factors do not greatly influence the rate of LOH at APC or explain inter differential susceptibility to colorectal tumours.

  14. Assignment of human sprouty 4 gene to chromosome segment 5q32∼33 and analysis of its pattern of expression

    Indian Academy of Sciences (India)

    Hua Liu; Jin-Zhong Chen; Shao-Hua Gu; Jian-Liang Dai; En-Pang Zhao; Lu Huang; Wang-Xiang Xu; Yi Xie; Yu-Min Mao

    2003-04-01

    The human sprouty 4 (SPYR4) gene was localized to chromosome band 5q32∼33 by screening the Stanford radiation hybrid G3 panel using a SPRY4-specific primer pair for PCR. Northern blot analysis revealed two different mRNAs (5 kb and 2 kb) in liver, skeletal muscle, heart, lung, kidney, spleen, placenta and small intestine. Reverse transcriptase-PCR analysis showed that SPYR4 was expressed in all tested tissues to different levels.

  15. The human and mouse receptors of hyaluronan-mediated motility, RHAMM, genes (HMMR) map to human chromosome 5q33.2-qter and mouse chromosome 11

    Energy Technology Data Exchange (ETDEWEB)

    Spicer, A.P.; McDonald, J.A. [Mayo Clinic Scottsdale, AZ (United States); Roller, M.L.; Camper, S.A. [Univ. of Michigan Medical School, Ann Arbor, MI (United States)] [and others

    1995-11-01

    The gene for the receptor for hyaluronan-mediated motility, RHAAM (designated hyaluronan-mediated motility receptor, HMMR (human) and Hmmr (mouse), for mapping purposes), was localized to human chromosome 5q33.2-qter by somatic cell and radiation hybrid analyses. Investigation of two interspecific back-crosses localized the mouse RHAMM (Hmmr) locus 18 cM from the centromere of mouse chromosome 11 within a region of synteny homology with human chromosome 5q23-q35 genes. The map position of the human RHAMM gene places it in a region comparatively rich in disease-associated genes, including those for low-frequency hearing loss, dominant limb-girdle muscular dystrophy, diastrophic dysplasia, Treacher Collins syndrome, and myeloid disorders associated with the 5q-syndrome. The RHAMM gene location and its ability to transform cells when overexpressed implicate RHAMM as a possible candidate gene in the pathogenesis of the recently described t(5;14)(q33-q34;q11) acute lymphoblastic leukemias. 18 refs., 1 fig.

  16. The spinal muscular atrophy gene region at 5q13.1 has a paralogous chromosomal region at 6p21.3.

    Science.gov (United States)

    Banyer, J L; Goldwurm, S; Cullen, L; van der Griend, B; Zournazi, A; Smit, D J; Powell, L W; Jazwinska, E C

    1998-03-01

    Paralogous regions are duplicated segments of chromosomal DNA that have been acquired during the evolution of the genome. Subsequent divergent evolution of the genes within paralogous regions can lead to the formation of gene families. Here, we report the identification of a region on Chromosome (Chr) 6 at 6p21.3 that is paralogous with the Spinal Muscular Atrophy (SMA) gene region on Chr 5 at 5q13.1. Partial characterization of this region identified nine sequences all of which are highly homologous to DNA sequences of the SMA gene region at 5q13.1. These sequences include four beta-glucuronidase sequences, two retrotransposon sequences, a novel cDNA, a Sequence Tagged Site (STS), and one that is homologous to exon 9 of the Neuronal Apoptosis Inhibitor Protein (NAIP) gene. The 6p21.3 paralogous SMA region may contain genes that are related to those in the SMA region at 5q13.1; however, a direct association of this region with SMA is unlikely given that no linkage of SMA with Chr 6 has been reported.

  17. Fine-scale mapping of the 5q11.2 breast cancer locus reveals at least three independent risk variants regulating MAP3K1

    DEFF Research Database (Denmark)

    Glubb, Dylan M; Maranian, Mel J; Michailidou, Kyriaki

    2015-01-01

    Genome-wide association studies (GWASs) have revealed SNP rs889312 on 5q11.2 to be associated with breast cancer risk in women of European ancestry. In an attempt to identify the biologically relevant variants, we analyzed 909 genetic variants across 5q11.2 in 103,991 breast cancer individuals an...

  18. Localized Multistreams for P2P Streaming

    Directory of Open Access Journals (Sweden)

    Majed Alhaisoni

    2010-01-01

    Full Text Available Streaming video over the Internet, including cellular networks, has now become a commonplace. Network operators typically use multicasting or variants of multiple unicasting to deliver streams to the user terminal in a controlled fashion. P2P streaming is an emerging alternative, which is theoretically more scalable but suffers from other issues arising from the dynamic nature of the system. Users' terminals become streaming nodes but they are not constantly connected. Another issue is that they are based on logical overlays, which are not optimized for the physical underlay infrastructure. An important proposition is to find effective ways to increase the resilience of the overlay whilst at the same time not conflicting with the network. In this article we look at the combination of two techniques, redundant streaming and locality awareness, in the context of both live and video-on-demand streaming. We introduce a new technique and assess it via a comparative, simulation-based study. We find that redundancy affects network utilization only marginally if traffic is kept at the edges via localization techniques.

  19. Where are $\\chi_{cJ}(3P)$?

    CERN Document Server

    Chen, Dian-Yong

    2016-01-01

    In the present work, we propose the $Y(4140)$ as the $\\chi_{c1}(3P)$ state by studying the $\\chi_{c1} \\pi^+ \\pi^-$ invariant mass spectrum of the $B\\to K \\chi_{c1} \\pi^+ \\pi^-$ process. In the $D\\bar{D}$ invariant mass spectrum of the $B\\to K D\\bar{D}$ process, we find a new resonance with the mass and width to be $ (4083.0 \\pm 5.0) $ and $ (24.1 \\pm 15.4) $ MeV, respectively, which could be a good candidate of the $\\chi_{c0}(3P)$ state. The theoretical investigations on the decay behaviors of the $\\chi_{cJ}(3P)$ in the present work support the assignments of the $Y(4140)$ and $Y(4080)$ as the $\\chi_{c1}(3P)$ and $\\chi_{c0}(3P)$ states, respectively. In addition, the $\\chi_{c2}(3P)$ state is predicted to be a very narrow state. The results in the present work could be tested by further experiments in the LHCb and forthcoming Belle II.

  20. The ViP2P Platform: XML Views in P2P

    CERN Document Server

    Karanasos, Konstantinos; Manolescu, Ioana; Zoupanos, Spyros

    2011-01-01

    The growing volumes of XML data sources on the Web or produced by enterprises, organizations etc. raise many performance challenges for data management applications. In this work, we are concerned with the distributed, peer-to-peer management of large corpora of XML documents, based on distributed hash table (or DHT, in short) overlay networks. We present ViP2P (standing for Views in Peer-to-Peer), a distributed platform for sharing XML documents based on a structured P2P network infrastructure (DHT). At the core of ViP2P stand distributed materialized XML views, defined by arbitrary XML queries, filled in with data published anywhere in the network, and exploited to efficiently answer queries issued by any network peer. ViP2P allows user queries to be evaluated over XML documents published by peers in two modes. First, a long-running subscription mode, when a query can be registered in the system and receive answers incrementally when and if published data matches the query. Second, queries can also be asked...

  1. Economic evaluation of lenalidomide (Revlimid® for the treatment of mielodysplastic syndrome with 5q deletion at low/intermediate-1 risk

    Directory of Open Access Journals (Sweden)

    Christian Ramos

    2017-06-01

    Full Text Available Background: Lenalidomide is an effective treatment for patients with lower risk myelodysplastic syndrome (MDS del5q. It reduces disease-related transfusion requirements, inhibits the malignant clone and causes a delay in tumor growth. There are also benefits in terms of quality of life and use of other health care resources. Objective: To perform an economic evaluation of lenalidomide for transfusion-dependent patients with Low/ Intermediate-1-risk MDS del 5q from the perspective of the public health care sector in Mexico. Material and methods: We developed a cost-effectiveness analysis using a Markov model of 5 stages: MDS transfusion- dependence, MDS transfusion-independence, complications from transfusion, acute myeloid leukemia, and death. The analyzed period was 5 years. Discount rate of 5% after year 1 was applied. Efficacy was elicited from a phase III clinical trial, MDS-004, which shows lenalidomide benefit in patients achieving transfusion-independence. There were also benefits with lenalidomide related to extended overall survival and decreased risk of acute myeloid leukemia. Unitary cost are from public price list of IMSS, and resources use was calculated from a Delphi panel methodology with clinical hematologists. A multivariate sensitivity analysis using a Monte Carlo technique was performed. Results: Over a 5-year period, each patient on lenalidomide avoided 487 days of transfusion, on average. The incremental cost per life-year without transfusion dependence is USD$14,072 (Exchange Rate = 1USD = 17.55 MXN. According to the sensitivity analysis the model is robust. If all expected patients are treated, their cost would be ~USD$19.7 million, which represents 0.18% of the total budget in the public health sector. Conclusions: Lenalidomide provides important clinical benefits in the treatment of patients with lower risk myelodysplastic syndromes with del5q, with a limited economic impact and with a cost per life living without blood

  2. Lenalidomide with or without erythropoietin in transfusion-dependent erythropoiesis-stimulating agent-refractory lower-risk MDS without 5q deletion.

    Science.gov (United States)

    Toma, A; Kosmider, O; Chevret, S; Delaunay, J; Stamatoullas, A; Rose, C; Beyne-Rauzy, O; Banos, A; Guerci-Bresler, A; Wickenhauser, S; Caillot, D; Laribi, K; De Renzis, B; Bordessoule, D; Gardin, C; Slama, B; Sanhes, L; Gruson, B; Cony-Makhoul, P; Chouffi, B; Salanoubat, C; Benramdane, R; Legros, L; Wattel, E; Tertian, G; Bouabdallah, K; Guilhot, F; Taksin, A L; Cheze, S; Maloum, K; Nimuboma, S; Soussain, C; Isnard, F; Gyan, E; Petit, R; Lejeune, J; Sardnal, V; Renneville, A; Preudhomme, C; Fontenay, M; Fenaux, P; Dreyfus, F

    2016-04-01

    After failure of erythropoiesis-stimulating agents (ESAs), lenalidomide (LEN) yields red blood cell (RBC) transfusion independence (TI) in 20-30% of lower-risk non-del5q myelodysplastic syndrome (MDS). Several observations suggest an additive effect of ESA and LEN in this situation. We performed a randomized phase III study in 131 RBC transfusion-dependent (TD, median transfusion requirement six RBC units per 8 weeks) lower-risk ESA-refractory non-del5q MDS. Patients received LEN alone, 10 mg per day, 21 days per 4 weeks (L arm) or LEN (same schedule) + erythropoietin (EPO) beta, 60,000 U per week (LE arm). In an intent-to-treat (ITT) analysis, erythroid response (HI-E, IWG 2006 criteria) after four treatment cycles (primary end point) was 23.1% (95% CI 13.5-35.2) in the L arm and 39.4% (95% CI 27.6-52.2) in the LE arm (P=0.044), while RBC-TI was reached in 13.8 and 24.2% of the patients in the L and LE arms, respectively (P=0.13). Median response duration was 18.1 and 15.1 months in the L and LE arms, respectively (P=0.47). Side effects were moderate and similar in the two arms. Low baseline serum EPO level and a G polymorphism of CRBN gene predicted HI-E. Combining LEN and EPO significantly improves erythroid response over LEN alone in lower-risk non-del5q MDS patients with anemia resistant to ESA.

  3. Diagnosis of del(5q MDS, 14 years after JAK-2 positive PV appearance: complete remission of both diseases with lenalidomide monotherapy

    Directory of Open Access Journals (Sweden)

    Antonella Vaccarino

    2016-10-01

    Full Text Available This is the report of the clinical case of a patient who presents the association of a JAK-2 positive chronic myeloproliferative neoplasia to a subsequent 5q- myelodysplastic syndrome, developed after about 14 years from the first diagnosis. Patient’s symptoms had rapidly worsened, and she became transfusion-dependent. Therapy with low-dose Lenalidomide quickly reduced the splenomegaly, and completely brought white cells counts, haemoglobin and platelets back to normal.  After more than one year from start, blood cell count is still normal. As far as we know this is the first case of an effective treatment with Lenalidomide reported in this clinical setting.

  4. Diagnosis of del(5q) MDS, 14 Years after JAK-2 Positive PV Appearance: Complete Remission of both Diseases with Lenalidomide Monotherapy.

    Science.gov (United States)

    Vaccarino, Antonella; Dogliotti, Irene; Marletto, Fabio; Demarchi, Andrea; Bazzan, Mario

    2016-01-01

    This is the report of the clinical case of a patient who presents the association of a JAK-2 positive chronic myeloproliferative neoplasia to a subsequent 5q- myelodysplastic syndrome, developed after about 14 years from the first diagnosis. Patient's symptoms had rapidly worsened, and she became transfusion-dependent. Therapy with low-dose Lenalidomide quickly reduced the splenomegaly and completely brought white cells counts, haemoglobin, and platelets back to normal. After more than one year from the start, blood cell count is still normal. As far as we know, this is the first case of an effective treatment with Lenalidomide reported in this clinical setting.

  5. Narrowing the position of the Treacher Collins syndrome locus to a small interval between three new microsatellite markers at 5q32-33. 1

    Energy Technology Data Exchange (ETDEWEB)

    Dixon, M.J.; Dixon, J. (Univ. of Manchester (United Kingdom)); Houseal, T.; Klinger, K.; Landes, G.M. (Integrated Genetics, Inc., Framingham, MA (United States)); Bhatt, M.; Ward, D.C. (Yale Univ. School of Medicine, New Haven (United States))

    1993-05-01

    Treacher Collins syndrome (TCOF1) is an autosomal dominant disorder of craniofacial development, the features of which include conductive hearing loss and cleft palate. The TCOF1 locus has been localized to chromosome 5q32-33.2. In the present study the authors have used the combined techniques of genetic linkage analysis and fluorescence in situ hybridization (FISH) to more accurately define the TCOF1 critical region. Cosmids IG90 and SPARC, which map to distal 5q, encompass two and one hypervariable microsatellite markers, respectively. The heterozygosity values of these three markers range from .72 to .81. Twenty-two unrelated TCOF1 families have been analyzed for linkage to these markers. There is strong evidence demonstrating linkage to all three markers, the strongest support for positive linkage being provided by haplotyping those markers at the locus encompassed by the cosmid IG90 (Z[sub max]= 19.65; 0 = .010). FISH to metaphase chromosomes and interphase nuclei established that IG90 lies centromeric to SPARC. This information combined with the data generated by genetic linkage analysis demonstrated that the TCOF1 locus is closely flanked proximally by IG90 and distally by SPARC. 30 refs., 2 figs., 4 tabs.

  6. A physical map of 15 loci on human chromosome 5q23-q33 by two-color fluorescence in situ hybridization

    Energy Technology Data Exchange (ETDEWEB)

    Saltman, D.L.; Dolganov, G.M. (Genelabs Inc. Redwood City, CA (United States)); Warrington, J.A.; Wasmuth, J.J. (Univ. of California, Irvine (United States)); Lovett, M. (Univ. of Texas Southwestern Medical Center, Dallas (United States))

    1993-06-01

    The q23-q33 region of human chromosome 5 encodes a large number of growth factors, growth factor receptors, and hormone/neurotransmitter receptors. This is also the general region into which several disease genes have been mapped, including diastrophic dysplasia, Treacher Collins syndrome, hereditary startle disease, the myeloid disorders that are associated with the 5q-syndrome, autosomal-dominant forms of hereditary deafness, and limb girdle muscular dystrophy. The authors have developed a framework physical map of this region using cosmid clones isolated from the Los Alamos arrayed chromosome 5-specific library. Entry points into this library included 14 probes to genes within this interval and one anonymous polymorphic marker locus. A physical map has been constructed using fluorescence in situ hybridization of these cosmids on metaphase and interphase chromosomes, and this is in good agreement with the radiation hybrid map of the region. The derived order of loci across the region is cen-IL4-IL5-IRF1-IL3-IL9-EGR1-CD14-FGFA-GRL-D5S207-ADRB2-SPARC-RPS14-CSF1R-ADRA1, and the total distance spanned by these loci is approximately 15 Mb. The framework map, genomic clones, and contig expansion within 5q23-q33 should provide valuable resources for the eventual isolation of the clinically relevant loci that reside in this region. 31 refs., 3 figs., 2 tabs.

  7. Chromosome 5q candidate genes in coeliac disease: genetic variation at IL4, IL5, IL9, IL13, IL17B and NR3C1.

    Science.gov (United States)

    Ryan, A W; Thornton, J M; Brophy, K; Daly, J S; McLoughlin, R M; O'Morain, C; Abuzakouk, M; Kennedy, N P; Stevens, F M; Feighery, C; Kelleher, D; McManus, R

    2005-02-01

    Genetic predisposition to coeliac disease (CD) is determined primarily by alleles at the HLA-DQB locus, and evidence exists implicating other major histocompatibility complex-linked genes (6p21) and the CTLA4 locus on chromosome 2q33. In addition, extensive family studies have provided strong, reproducible evidence for a susceptibility locus on chromosome 5q (CELIAC2). However, the gene responsible has not been identified. We have assayed genetic variation at the IL4, IL5, IL9, IL13, IL17B and NR3C1 (GR) loci, all of which are present on chromosome 5q and have potential or demonstrated involvement in autoimmune and/or inflammatory disease, in a sample of 409 CD cases and 355 controls. Thirteen single nucleotide polymorphisms were chosen on the basis of functional relevance, prior disease association and, where possible, prior knowledge of the haplotype variation present in European populations. There were no statistically significant allele or haplotype frequency differences between cases and controls. Therefore, these results provide no evidence that these loci are associated with CD in this sample population.

  8. A Rare Case of Extramedullary T/Myeloid Mixed Phenotype Acute Leukemia with t(1;5(q23;q33

    Directory of Open Access Journals (Sweden)

    Ahmad Monabati

    2016-01-01

    Full Text Available Mixed phenotype acute leukemia (MPAL is a rare neoplasm which accounts for 2–5% of all leukemias and it is classified under heading of acute leukemia of ambiguous lineage in 2008 WHO classification. This patient was a 61-year-old man who presented with malaise and weakness. In physical examination there was cervical and axillary lymphadenopathy. Paraclinical evaluation revealed anemia (Hb = 10.3 g/dL, MCV = 108 fl. Histologic sections of the axillary lymph node revealed leukemic involvement with two discrete populations of cells in immunohistochemistry. One population was immunoreactive for MPO and the other showed immunostaining for CD3, CD99, and tdt. Differential count of bone marrow cells in marrow aspirate had 6% blast. Karyotype study on bone marrow culture depicted an interesting finding which was t(1;5(q23;q33. An extensive search on literature was done for the same genetic change. A similar translocation has been mentioned in literature for other hematologic malignancies but not for same neoplasm; anyhow this translocation was an imbalanced one and led to der(5t(1;5(q12-25;q13-q35.

  9. Ypq3p-dependent histidine uptake by the vacuolar membrane vesicles of Saccharomyces cerevisiae.

    Science.gov (United States)

    Manabe, Kunio; Kawano-Kawada, Miyuki; Ikeda, Koichi; Sekito, Takayuki; Kakinuma, Yoshimi

    2016-06-01

    The vacuolar membrane proteins Ypq1p, Ypq2p, and Ypq3p of Saccharomyces cerevisiae are known as the members of the PQ-loop protein family. We found that the ATP-dependent uptake activities of arginine and histidine by the vacuolar membrane vesicles were decreased by ypq2Δ and ypq3Δ mutations, respectively. YPQ1 and AVT1, which are involved in the vacuolar uptake of lysine/arginine and histidine, respectively, were deleted in addition to ypq2Δ and ypq3Δ. The vacuolar membrane vesicles isolated from the resulting quadruple deletion mutant ypq1Δypq2Δypq3Δavt1Δ completely lost the uptake activity of basic amino acids, and that of histidine, but not lysine and arginine, was evidently enhanced by overexpressing YPQ3 in the mutant. These results suggest that Ypq3p is specifically involved in the vacuolar uptake of histidine in S. cerevisiae. The cellular level of Ypq3p-HA(3) was enhanced by depletion of histidine from culture medium, suggesting that it is regulated by the substrate.

  10. Prenatal diagnosis and molecular cytogenetic characterization of a 1.07-Mb microdeletion at 5q35.2–q35.3 associated with NSD1 haploinsufficiency and Sotos syndrome

    Directory of Open Access Journals (Sweden)

    Chih-Ping Chen

    2014-12-01

    Conclusion: Fetuses with Sotos syndrome may present macrocephaly, polyhydramnios, ventriculomegaly, and pyelectasis in the third trimester. aCGH and metaphase FISH are useful for rapid diagnosis of 5q35 microdeletion associated with Sotos syndrome.

  11. Circular RNA hsa_circ_0001564 facilitates tumorigenesis of osteosarcoma via sponging miR-29c-3p.

    Science.gov (United States)

    Li, Ji-Feng; Song, Yu-Ze

    2017-08-01

    Circular RNAs are a novel type of non-coding RNAs generated from back splicing, which has been verified to mediate multiple tumorigenesis. However, the role of circular RNA in osteosarcoma is still unclear. In this study, we preliminarily screened the circular RNAs expression profiles in osteosarcoma and investigated the potential regulation mechanism. The circular RNAs expression profiles in osteosarcoma were screened using circular RNA microarray analysis, and results showed that there were 1152 circular RNAs upregulated and 915 circular RNAs downregulated in tumor tissue compared to adjacent tissue. Hsa_circ_0001564, located at 5q35.3 and its associated gene symbol is CANX, was one of the significantly overexpressed circular RNAs in osteosarcoma tissue, as well as in osteosarcoma cell lines. In functional experiments, hsa_circ_001564 knockdown significantly suppressed the proliferation activity, induced cell-cycle arrest in G0/G1 phase, and promoted apoptosis in HOS and MG-63 cells. Subsequently, we explored the probable mechanism of hsa_circ_001564, and fortunately, bioinformatics analysis revealed that miR-29c-3p contained the complementary binding region with hsa_circ_0001564, which was confirmed by dual-luciferase reporter assay. Moreover, rescue experiments illustrated that miR-29c-3p could reverse the oncogenesis effect of hsa_circ_001564. Our study discovers that hsa_circ_0001564 acts as miR-29c-3p sponge to mediate the tumorigenicity, which could act as a potential biomarker for the osteosarcoma and provide a novel insight for competing endogenous RNA mechanism in osteosarcoma.

  12. Main: 1M2P [RPSD[Archive

    Lifescience Database Archive (English)

    Full Text Available 1M2P トウモロコシ Corn Zea mays L. Casein Kinase Ii, Alpha Chain Name=Ack2; Zea Mays Mole...LVGRHSRKPWLKFMNADNQHLVSPEAIDFLDKLLRYDHQERLTALEAMTHPYFQQVRAAENSRTRA corn_1M2P.jpg ...

  13. The gene for calcium-modulating cyclophilin ligand (CAMLG) is located on human Chromosome 5q23 and a syntenic region of mouse chromosome 13

    Energy Technology Data Exchange (ETDEWEB)

    Bram, R.J.; Valentine, V.; Shapiro, D.N. [St. Jude Children`s Research Hospital, Memphis, TN (United States)]|[Univ. of Tennessee, Memphis, TN (United States)] [and others

    1996-01-15

    The CAMLG gene encodes a novel cyclophilin B-binding protein called calcium-modulating cyclophilin ligand, which appears to be involved in the regulation of calcium signaling in T lymphocytes and other cells. The murine homolog, Caml, was localized by interspecific backcross analysis in the middle of chromosome 13. By fluorescence in situ hybridization, this gene was localized to human chromosome 5 in a region (q23) known to be syntenic to mouse chromosome 13. These results provide further evidence supporting the extensive homology between human chromosome 5q and mouse chromosome 13. In addition, the results will provide a basis for further evaluation of cytogenetic anomalies that may contribute to inherited defects in calcium signaling or immune system function. 15 refs., 2 figs.

  14. Myelodysplastic disorders carrying both isolated del(5q) and JAK2(V617F) mutation: concise review, with focus on lenalidomide therapy.

    Science.gov (United States)

    Musto, Pellegrino; Simeon, Vittorio; Guariglia, Roberto; Bianchino, Gabriella; Grieco, Vitina; Nozza, Filomena; La Rocca, Francesco; Marziano, Gioacchino; Lalinga, Anna Vittoria; Fabiani, Emiliano; Voso, Maria Teresa; Scaravaglio, Patrizia; Mecucci, Cristina; D'Arena, Giovanni

    2014-01-01

    The concomitant presence of del(5q) and JAK2(V617F) mutation is an infrequent event which occurs in rare patients with peculiar cytogenetic, molecular, morphological and clinical features, resembling those of both myelodysplastic syndromes and myeloproliferative neoplasms. Lenalidomide may induce rapid, profound, and long-lasting responses in a subset of these patients. However, the mechanism(s) by which the drug acts in these conditions remain not completely elucidated. A new case report and a review of all cases published so far in this setting are provided. Furthermore, the possibility of categorizing - from a clinical, pathological, and biological point of view - for at least some of these patients as a potential distinct entity is discussed.

  15. Myelodysplastic disorders carrying both isolated del(5q) and JAK2V617F mutation: concise review, with focus on lenalidomide therapy

    Science.gov (United States)

    Musto, Pellegrino; Simeon, Vittorio; Guariglia, Roberto; Bianchino, Gabriella; Grieco, Vitina; Nozza, Filomena; La Rocca, Francesco; Marziano, Gioacchino; Lalinga, Anna Vittoria; Fabiani, Emiliano; Voso, Maria Teresa; Scaravaglio, Patrizia; Mecucci, Cristina; D’Arena, Giovanni

    2014-01-01

    The concomitant presence of del(5q) and JAK2V617F mutation is an infrequent event which occurs in rare patients with peculiar cytogenetic, molecular, morphological and clinical features, resembling those of both myelodysplastic syndromes and myeloproliferative neoplasms. Lenalidomide may induce rapid, profound, and long-lasting responses in a subset of these patients. However, the mechanism(s) by which the drug acts in these conditions remain not completely elucidated. A new case report and a review of all cases published so far in this setting are provided. Furthermore, the possibility of categorizing – from a clinical, pathological, and biological point of view – for at least some of these patients as a potential distinct entity is discussed. PMID:24966686

  16. L-Leucine improves the anaemia in models of Diamond Blackfan anaemia and the 5q- syndrome in a TP53-independent way.

    Science.gov (United States)

    Narla, Anupama; Payne, Elspeth M; Abayasekara, Nirmalee; Hurst, Slater N; Raiser, David M; Look, A Thomas; Berliner, Nancy; Ebert, Benjamin L; Khanna-Gupta, Arati

    2014-11-01

    Haploinsufficiency of ribosomal proteins (RPs) and upregulation of the tumour suppressor TP53 have been shown to be the common basis for the anaemia observed in Diamond Blackfan anaemia and 5q- myelodysplastic syndrome. We previously demonstrated that treatment with L-Leucine resulted in a marked improvement in anaemia in disease models. To determine if the L-Leucine effect was Tp53-dependent, we used antisense MOs to rps19 and rps14 in zebrafish; expression of tp53 and its downstream target cdkn1a remained elevated following L-leucine treatment. We confirmed this observation in human CD34+ cells. L-Leucine thus alleviates anaemia in RP-deficient cells in a TP53-independent manner.

  17. Genetic and physical mapping of the Treacher Collins syndrome locus with respect to loci in the chromosome 5q3 region

    Energy Technology Data Exchange (ETDEWEB)

    Jabs, E.W.; Li, Xiang; Coss, C.; Taylor, E. (Johns Hopkins School of Medicine, Baltimore, MD (United States)); Lovett, M. (Univ. of Texas Southwestern Medical Center, San Antonio, TX (United States)); Yamaoka, L.H.; Speer, M.C. (Duke Univ. Medical Center, Durham, NC (United States)); Cadle, R.; Hall, B. (Univ. of Kentucky, Lexington, KY (United States)); Brown, K. (Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY (United States)) (and others)

    1993-10-01

    Treacher Collins syndrome is an autosomal dominant, craniofacial developmental disorder, and its locus (TCOF1) has been mapped to chromosome 5q3. To refine the location of the gene within this region, linkage analysis was performed among the TCOF1 locus and 12 loci (IL9, FGFA, GRL, D5S207, D5S210, D5S376, CSF1R, SPARC, D5S119, D5S209, D5S527, FGFR4) in 13 Treacher Collins syndrome families. The highest maximum lod score was obtained between loci TCOF1 and D5S210 (Z = 10.52; [theta] = 0.02 [+-] 0.07). The best order, IL9-GRL-D5S207/D5S210-CSF1R-SPARC-D5S119, and genetic distances among these loci were determined in the 40 CEPH families by multipoint linkage analysis. YAC clones were used to establish the order of loci, centromere-5[prime]GRL3[prime]-D5S207-D5S210-D5S376-CSF1R-SPARC-D5S119-telomere. By combining known physical mapping data with ours, the order of chromosome 5q3 markers is centomere-IL9-FGFA-5[prime]GRL3[prime]-D5s207-D5S210-D5S376-CSF1R-SPARC-D5S119-D5S209-FGFR4-telomere. Based on this order, haplotype analysis suggests that the TCOF1 locus resides distal CSF1R and proximal to SPARC within a region less than 1 Mb in size. 29 refs., 2 figs., 2 tabs.

  18. A replication study for three nephrolithiasis loci at 5q35.3, 7p14.3 and 13q14.1 in the Japanese population.

    Science.gov (United States)

    Yasui, Takahiro; Okada, Atsushi; Urabe, Yuji; Usami, Masayuki; Mizuno, Kentaro; Kubota, Yasue; Tozawa, Keiichi; Sasaki, Shoichi; Higashi, Yoshihito; Sato, Yoshikazu; Kubo, Michiaki; Nakamura, Yusuke; Matsuda, Koichi; Kohri, Kenjiro

    2013-09-01

    A previous genome-wide association study (GWAS) reported three novel nephrolithiasis-susceptibility loci at 5q35.3, 7p14.3 and 13q14.1. Here, we investigated the association of these loci with nephrolithiasis by using an independent Japanese sample set. We performed case-control association analysis using 601 patients with nephrolithiasis and 201 control subjects. We selected seven single-nucleotide polymorphisms (SNPs): rs12654812 and rs11746443 from 5q35.3 (RGS14-SLC34A1-PFN3-F12); rs12669187 and rs1000597 from 7p14.3 (INMT-FAM188B-AQP1); and rs7981733, rs1170155, and rs4142110 from 13q14.1 (DGKH (diacylglycerol kinase)), which were previously reported to be significantly associated with nephrolithiasis. rs12654812, rs12669187 and rs7981733 were significantly associated with nephrolithiasis after Bonferroni's correction (P=3.12 × 10(-3), odds ratio (OR)=1.43; P=6.40 × 10(-3), OR=1.57; and P=5.00 × 10(-3), OR=1.41, respectively). Meta-analysis of current and previous GWAS results indicated a significant association with nephrolithiasis (P=7.65 × 10(-15), 7.86 × 10(-14) and 1.06 × 10(-9), respectively). We observed a cumulative effect with these three SNPs; individuals with three or more risk alleles had a 5.9-fold higher risk for nephrolithiasis development than those with only one risk allele. Our findings elucidated the significance of genetic variation at these three loci in nephrolithiasis in the Japanese population.

  19. Myelodysplastic disorders carrying both isolated del(5q and JAK2V617F mutation: concise review, with focus on lenalidomide therapy

    Directory of Open Access Journals (Sweden)

    Musto P

    2014-06-01

    Full Text Available Pellegrino Musto,1 Vittorio Simeon,2 Roberto Guariglia,3 Gabriella Bianchino,4 Vitina Grieco,4 Filomena Nozza,4 Francesco La Rocca,2 Gioacchino Marziano,1 Anna Vittoria Lalinga,5 Emiliano Fabiani,6 Maria Teresa Voso,6 Patrizia Scaravaglio,7 Cristina Mecucci,8 Giovanni D'Arena31Scientific Direction, 2Laboratory of Preclinical and Translational Research, 3Unit of Hematology and Stem Cell Transplantation, 4Laboratory of Clinical Research and Advanced Diagnostics, 5Pathology Unit, IRCCS, Centro di Riferimento Oncologico della Basilicata, Rionero in Vulture, Italy; 6Department of Hematology, Universita Cattolica del Sacro Cuore, Rome, Italy; 7Laboratory of Internal Medicine and Hematology, S Luigi Gonzaga Hospital, Orbassano, Italy; 8Hematology and Bone Marrow Transplantation Unit, University of Perugia, Perugia, ItalyAbstract: The concomitant presence of del(5q and JAK2V617F mutation is an infrequent event which occurs in rare patients with peculiar cytogenetic, molecular, morphological and clinical features, resembling those of both myelodysplastic syndromes and myeloproliferative neoplasms. Lenalidomide may induce rapid, profound, and long-lasting responses in a subset of these patients. However, the mechanism(s by which the drug acts in these conditions remain not completely elucidated. A new case report and a review of all cases published so far in this setting are provided. Furthermore, the possibility of categorizing – from a clinical, pathological, and biological point of view – for at least some of these patients as a potential distinct entity is discussed.Keywords: myelodysplastic syndromes, myeloproliferative neoplasms, lenalidomide, del(5q, JAK2, World Health Organization

  20. Local genotype influences DNA methylation at two asthma-associated regions, 5q31 and 17q21, in a founder effect population.

    Science.gov (United States)

    Al Tuwaijri, Abeer; Gagné-Ouellet, Valérie; Madore, Anne-Marie; Laprise, Catherine; Naumova, Anna K

    2016-04-01

    Two asthma-associated regions 17q12-q21 and 5q31.1 harbour genes that show strong effect of genotype on expression levels. DNA methylation has an important role in gene regulation; therefore, we examined DNA methylation at promoters of 12 genes from 5q31 and 17q12-q21 regions. Our goal was to determine whether DNA methylation was associated with predisposition to asthma and whether such a relationship was independent from genetic association. Using sodium bisulfite sequencing and pyrosequencing methylation assays, we examined the effect of genotype on DNA methylation in peripheral blood cells from individuals from the Saguenay-Lac-Saint-Jean asthma familial collection and lymphoblastoid cell lines. The local genotype influenced methylation levels of solute carrier family 22 (organic 3 cation/carnitine transporter) member 5 (SLC22A5), zona pellucida binding protein 2 (ZPBP2) and gasdermin A (GSDMA) promoter regions. The genotype had a dominant effect on ZPBP2 and GSDMA methylation with lower methylation levels in individuals that carry the asthma-predisposing alleles. Males also had lower methylation at the ZPBP2 promoter than females. We did not observe an effect of asthma status that would be independent of the genotype and the sex effects in the GSDMA, ZPBP2 and SLC22A5 regions; however, GSDMA and ZPBP2 data were suggestive of interaction between asthma and methylation levels in females and SLC22A5 in males. The local genotype influences methylation levels at SLC22A5 and ZPBP2 promoters independently of the asthma status. Further studies are necessary to confirm the relationship between GSDMA-ZPBP2 and SLC22A5 methylation and asthma in females and males separately. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  1. Is the X(3915) the chi_{c0}(2P)?

    CERN Document Server

    Olsen, Stephen Lars

    2014-01-01

    The Particle Data Group has assigned the X(3915) meson, an omega J/psi mass peak seen in B-> omega J/psi decays and in two-photon fusion reactions gamma-gamma->omega J/psi, as the chi_{c0}(2P), the 2^3P_0 charmonium state. Here it is shown that if the X(3915) is the chi_{c0}(2P), the measured strength of the gamma-gamma->X(3915) signal implies an upper limit on the branching fraction Bf(chi_{c0}(2P)->omega J/psi)14.3% lower limit derived for the same quantity from the B-> K X(3915) decay rate. Also, the absence any signal for X(3915)-> D0 D0-bar in B+ -> K+ D0 D0-bar decays is used to establish the limit Bf(X(3915)->D0 D0-bar) omega J/psi). This contradicts expectations that chi_{c0}(2P) decays to D0 D0-bar would be a dominant process, while decays to omega J/psi, which are Okubo-Zweig-Iizuka suppressed, would be relatively rare. These, plus reasons given earlier by Guo and Meissner, raise serious doubts about the X(3915)=chi_{c0}(2P) assignment.

  2. Providing VoD Streaming Using P2P Networks

    Science.gov (United States)

    Pedro Muñoz-Gea, Juan; Malgosa-Sanahuja, Josemaria; Manzanares-Lopez, Pilar; Carlos Sanchez-Aarnoutse, Juan

    Overlays and P2P systems, initially developed to support IP multicast and file-sharing, have moved beyond that functionality. They are also proving to be key technologies for the delivery of video streaming. Recently, there have been a number of successful deployments for "live" P2P streaming. However, the question remains open whether similar P2P technologies can be used to provide VoD (Video-On-Demand) services. A P2P VoD service is more challenging to design than a P2P live streaming system because the system should allow users arriving at arbitrary times to watch (arbitrary parts of) the video.

  3. Risk Management of P2P Internet Financing Service Platform

    Science.gov (United States)

    Yalei, Li

    2017-09-01

    Since 2005, the world’s first P2P Internet financing service platform Zopa in UK was introduced, in the development of “Internet +” trend, P2P Internet financing service platform has been developed rapidly. In 2007, China’s first P2P platform “filming loan” was established, marking the P2P Internet financing service platform to enter China and the rapid development. At the same time, China’s P2P Internet financing service platform also appeared in different forms of risk. This paper focuses on the analysis of the causes of risk of P2P Internet financing service platform and the performance of risk management process. It provides a solution to the Internet risk management plan, and explains the risk management system of the whole P2P Internet financing service platform and the future development direction.

  4. Network-Aware DHT-Based P2P Systems

    Science.gov (United States)

    Fayçal, Marguerite; Serhrouchni, Ahmed

    P2P networks lay over existing IP networks and infrastructure. This chapter investigates the relation between both layers, details the motivations for network awareness in P2P systems, and elucidates the requirements P2P systems have to meet for efficient network awareness. Since new P2P systems are mostly based on DHTs, we also present and analyse DHT-based architectures. And after a brief presentation of different existing network-awareness solutions, the chapter goes on effective cooperation between P2P traffic and network providers' business agreements, and introduces emerging DHT-based P2P systems that are network aware through a semantic defined for resource sharing. These new systems ensure also a certain context-awareness. So, they are analyzed and compared before an open end on prospects of network awareness in P2P systems.

  5. Gluon fragmentation into {sup 3} P{sub J} quarkonium

    Energy Technology Data Exchange (ETDEWEB)

    Ma, J.P.

    1995-10-01

    The functions of the gluon fragmentation into {sup 3}P{sub j} quarkonium are calculated to order {alpha}{sup 2}{sub s}. With the recent progress in analysing quarkonium systems in QCD it is possible show how the so called divergence in the limit of the zero-binding energy, which is related to P-wave quarkonia, is treated correctly in the case of fragmentation functions. The obtained fragmentation functions satisfy explicitly at the order of {alpha} {sup 2}{sub s} the Altarelli-Parisi equation and when z {yields} 0 they behave as z{sup -1} as expected. 19 refs., 7 figs.

  6. Infantile spasm associated with 5q14.3 microdeletion syndrome: clinical and genetic characterization of a core family%5q14.3微缺失综合征导致婴儿痉挛症一家系的临床及遗传学研究

    Institute of Scientific and Technical Information of China (English)

    俞冬熠; 李朔; 姜楠

    2015-01-01

    目的 分析婴儿痉挛症患儿的临床特征,并对患儿及其父母进行遗传学检测.方法 分析2014年3月接诊的1例婴儿痉挛症患儿面部特征、体格和智力发育状况.实验室检查采用常规G显带分析患儿及父母外周血染色体核型,单核苷酸多态性(SNP)基因芯片技术检测染色体微小改变,荧光原位杂交技术(FISH)验证结果,并对其父母染色体中期分裂象进行FISH检测.结果 患儿女,7岁,语言、运动、语言发育严重迟滞,生后4个月出现癫痫发作,临床诊断为“婴儿痉挛症”.体检见消瘦,面容特殊,存在刻板样不自主动作.头颅CT平扫提示“硬膜下积液”.脑电图检查提示:痫样放电频繁;高度失律.患儿及其父母常规染色体核型分析(400条带)均显示为正常核型.SNP微阵列技术检测显示该患儿染色体5q14.3区段微缺失,缺失片段为2.03 Mb,分子核型为arr5q14.3(87 538 430 ~ 89 565 757)× 1,该区段包含MEF2C基因.选择区域特异性RP11-293 L20探针,应用FISH技术对芯片结果进行验证,确认患儿5q14.3区域存在杂合缺失.父母染色体核型正常,无5q14.3区域缺失,无插入突变.结论 患儿染色体5q14.3区段发生缺失为婴儿痉挛症的病因.患儿为新发生的5q14.3微缺失综合征,父母再次生育时,建议选择SNP芯片技术进行产前诊断.%Objective To characterize the clinical feature of a child with infantile spasm,karyotype and molecular cytogenetic analyses were performed to investigate the cause of disease and choose a suitable prenatal diagnostic method for the couple with the child.Method Routine G-banding was performed to analyze the karyotype of the patient and her parents,and molecular karyotyping was performed using SNP array.Confirmation and segregation studies were performed by fluorescence in situ hybridization (FISH).Result The patient presented with severe psychomotor retardation,epilepsy,muscular hypotonia,stereotypic behavior and

  7. 伴有t(3;5)(q25;q34)的骨髓增生异常综合征急性髓系白血病变患者的分子特征%Molecular characterization of t (3;5)(q25;q34) in a case of acute myeloid leukemia secondary to myelodysplastic syndromes

    Institute of Scientific and Technical Information of China (English)

    张悦; 邵英起; 薛永权; 王一; 张美荣; 刘旭平; 郑以州; 肖志坚

    2006-01-01

    目的 研究t(3;5)(q25;q34)的分子特征.方法 R显带染色体核型分析和染色体涂染分析确定t(3;5)(q25;q34)存在.RT-PCR法检测患者NPM-MLF1融合基因、MLF1基因表达.PCR联合序列分析检测NPM基因突变.实时定量RT-PCR(Real-time PCR)检测Evi-1,MDS1/Evi-1表达.免疫组化染色确定MLF1和NPM-MLF1蛋白细胞定位分布.Western blot法检测bcl-2蛋白表达.结果 染色体核型分析和涂染分析均证实存在染色体t(3;5)(q25;q34),NPM-MLF1融合基因阳性,经诱导治疗获得完全缓解后转为阴性.患者白血病细胞仅表达MLF1基因非编码蛋白剪接体,不表达Evi-1基因,弱表达MDS1/Evi-1.无NPM基因突变,未检出bcl-2蛋白表达.MLF1蛋白定位于细胞质,而NPM-MLF1则主要定位于胞核.结论 t(3;5)(q25;q34)是髓系肿瘤的一种少见的染色体易位,该染色体易位导致形成NPM-MLF1融合基因是其发病的分子学基础.

  8. Products of the Benzene + O(3P) Reaction

    Energy Technology Data Exchange (ETDEWEB)

    Taatjes, Craig A.; Osborn, David L.; Selby, Talitha M.; Meloni, Giovanni; Trevitt, Adam J.; Epifanovsky, Evgeny; Krylov, Anna I.; Sirjean, Baptiste; Dames, Enoch; Wang, Hai

    2009-12-21

    The gas-phase reaction of benzene with O(3P) is of considerable interest for modeling of aromatic oxidation, and also because there exist fundamental questions concerning the prominence of intersystem crossing in the reaction. While its overall rate constant has been studied extensively, there are still significant uncertainties in the product distribution. The reaction proceeds mainly through the addition of the O atom to benzene, forming an initial triplet diradical adduct, which can either dissociate to form the phenoxy radical and H atom, or undergo intersystem crossing onto a singlet surface, followed by a multiplicity of internal isomerizations, leading to several possible reaction products. In this work, we examined the product branching ratios of the reaction between benzene and O(3P) over the temperature range of 300 to 1000 K and pressure range of 1 to 10 Torr. The reactions were initiated by pulsed-laser photolysis of NO2 in the presence of benzene and helium buffer in a slow-flow reactor, and reaction products were identified by using the multiplexed chemical kinetics photoionization mass spectrometer operating at the Advanced Light Source (ALS) of Lawrence Berkeley National Laboratory. Phenol and phenoxy radical were detected and quantified. Cyclopentadiene and cyclopentadienyl radical were directly identified for the first time. Finally, ab initio calculations and master equation/RRKM modeling were used to reproduce the experimental branching ratios, yielding pressure-dependent rate expressions for the reaction channels, including phenoxy + H, phenol, cyclopentadiene + CO, which are proposed for kinetic modeling of benzene oxidation.

  9. Thermoelectric properties of polycrystalline NiSi3P4

    Science.gov (United States)

    May, Andrew F.; McGuire, Michael A.; Wang, Hsin

    2013-03-01

    The Hall and Seebeck coefficients, electrical resistivity, and thermal conductivity of polycrystalline NiSi3P4 were characterized from 2 to 775 K. Undoped NiSi3P4 behaves like a narrow gap semiconductor, with activated electrical resistivity ρ below room temperature and a large Seebeck coefficient of ˜400 μV/K at 300 K. Attempts to substitute boron for silicon resulted in the production of extrinsic holes, yielding moderately doped semiconductor behavior with ρ increasing with increasing temperature above ˜150 K. Hall carrier densities are limited to approximately 5 × 1019 cm-3 at 200 K, which would suggest the solubility limit of boron is reached if boron is indeed incorporated into the lattice. These extrinsic samples have a Hall mobility of ˜12 cm2/V/s at 300 K, and a parabolic band equivalent effective mass of ˜3.5 times the free electron mass. At 700 K, the thermoelectric figure of merit zT reaches ˜0.1. Further improvements in thermoelectric performance would require reaching higher carrier densities, as well as a mechanism to further reduce the lattice thermal conductivity, which is ˜5 W/m/K at 700 K. Alloying in Ge results in a slight reduction of the thermal conductivity at low temperatures, with little influence observed at higher temperatures.

  10. Fine-scale mapping of the 5q11.2 breast cancer locus reveals at least three independent risk variants regulating MAP3K1.

    Science.gov (United States)

    Glubb, Dylan M; Maranian, Mel J; Michailidou, Kyriaki; Pooley, Karen A; Meyer, Kerstin B; Kar, Siddhartha; Carlebur, Saskia; O'Reilly, Martin; Betts, Joshua A; Hillman, Kristine M; Kaufmann, Susanne; Beesley, Jonathan; Canisius, Sander; Hopper, John L; Southey, Melissa C; Tsimiklis, Helen; Apicella, Carmel; Schmidt, Marjanka K; Broeks, Annegien; Hogervorst, Frans B; van der Schoot, C Ellen; Muir, Kenneth; Lophatananon, Artitaya; Stewart-Brown, Sarah; Siriwanarangsan, Pornthep; Fasching, Peter A; Ruebner, Matthias; Ekici, Arif B; Beckmann, Matthias W; Peto, Julian; dos-Santos-Silva, Isabel; Fletcher, Olivia; Johnson, Nichola; Pharoah, Paul D P; Bolla, Manjeet K; Wang, Qin; Dennis, Joe; Sawyer, Elinor J; Tomlinson, Ian; Kerin, Michael J; Miller, Nicola; Burwinkel, Barbara; Marme, Frederik; Yang, Rongxi; Surowy, Harald; Guénel, Pascal; Truong, Thérèse; Menegaux, Florence; Sanchez, Marie; Bojesen, Stig E; Nordestgaard, Børge G; Nielsen, Sune F; Flyger, Henrik; González-Neira, Anna; Benitez, Javier; Zamora, M Pilar; Arias Perez, Jose Ignacio; Anton-Culver, Hoda; Neuhausen, Susan L; Brenner, Hermann; Dieffenbach, Aida Karina; Arndt, Volker; Stegmaier, Christa; Meindl, Alfons; Schmutzler, Rita K; Brauch, Hiltrud; Ko, Yon-Dschun; Brüning, Thomas; Nevanlinna, Heli; Muranen, Taru A; Aittomäki, Kristiina; Blomqvist, Carl; Matsuo, Keitaro; Ito, Hidemi; Iwata, Hiroji; Tanaka, Hideo; Dörk, Thilo; Bogdanova, Natalia V; Helbig, Sonja; Lindblom, Annika; Margolin, Sara; Mannermaa, Arto; Kataja, Vesa; Kosma, Veli-Matti; Hartikainen, Jaana M; Wu, Anna H; Tseng, Chiu-chen; Van Den Berg, David; Stram, Daniel O; Lambrechts, Diether; Zhao, Hui; Weltens, Caroline; van Limbergen, Erik; Chang-Claude, Jenny; Flesch-Janys, Dieter; Rudolph, Anja; Seibold, Petra; Radice, Paolo; Peterlongo, Paolo; Barile, Monica; Capra, Fabio; Couch, Fergus J; Olson, Janet E; Hallberg, Emily; Vachon, Celine; Giles, Graham G; Milne, Roger L; McLean, Catriona; Haiman, Christopher A; Henderson, Brian E; Schumacher, Fredrick; Le Marchand, Loic; Simard, Jacques; Goldberg, Mark S; Labrèche, France; Dumont, Martine; Teo, Soo Hwang; Yip, Cheng Har; See, Mee-Hoong; Cornes, Belinda; Cheng, Ching-Yu; Ikram, M Kamran; Kristensen, Vessela; Zheng, Wei; Halverson, Sandra L; Shrubsole, Martha; Long, Jirong; Winqvist, Robert; Pylkäs, Katri; Jukkola-Vuorinen, Arja; Kauppila, Saila; Andrulis, Irene L; Knight, Julia A; Glendon, Gord; Tchatchou, Sandrine; Devilee, Peter; Tollenaar, Robert A E M; Seynaeve, Caroline; Van Asperen, Christi J; García-Closas, Montserrat; Figueroa, Jonine; Chanock, Stephen J; Lissowska, Jolanta; Czene, Kamila; Klevebring, Daniel; Darabi, Hatef; Eriksson, Mikael; Hooning, Maartje J; Hollestelle, Antoinette; Martens, John W M; Collée, J Margriet; Hall, Per; Li, Jingmei; Humphreys, Keith; Shu, Xiao-Ou; Lu, Wei; Gao, Yu-Tang; Cai, Hui; Cox, Angela; Cross, Simon S; Reed, Malcolm W R; Blot, William; Signorello, Lisa B; Cai, Qiuyin; Shah, Mitul; Ghoussaini, Maya; Kang, Daehee; Choi, Ji-Yeob; Park, Sue K; Noh, Dong-Young; Hartman, Mikael; Miao, Hui; Lim, Wei Yen; Tang, Anthony; Hamann, Ute; Torres, Diana; Jakubowska, Anna; Lubinski, Jan; Jaworska, Katarzyna; Durda, Katarzyna; Sangrajrang, Suleeporn; Gaborieau, Valerie; Brennan, Paul; McKay, James; Olswold, Curtis; Slager, Susan; Toland, Amanda E; Yannoukakos, Drakoulis; Shen, Chen-Yang; Wu, Pei-Ei; Yu, Jyh-Cherng; Hou, Ming-Feng; Swerdlow, Anthony; Ashworth, Alan; Orr, Nick; Jones, Michael; Pita, Guillermo; Alonso, M Rosario; Álvarez, Nuria; Herrero, Daniel; Tessier, Daniel C; Vincent, Daniel; Bacot, Francois; Luccarini, Craig; Baynes, Caroline; Ahmed, Shahana; Healey, Catherine S; Brown, Melissa A; Ponder, Bruce A J; Chenevix-Trench, Georgia; Thompson, Deborah J; Edwards, Stacey L; Easton, Douglas F; Dunning, Alison M; French, Juliet D

    2015-01-08

    Genome-wide association studies (GWASs) have revealed SNP rs889312 on 5q11.2 to be associated with breast cancer risk in women of European ancestry. In an attempt to identify the biologically relevant variants, we analyzed 909 genetic variants across 5q11.2 in 103,991 breast cancer individuals and control individuals from 52 studies in the Breast Cancer Association Consortium. Multiple logistic regression analyses identified three independent risk signals: the strongest associations were with 15 correlated variants (iCHAV1), where the minor allele of the best candidate, rs62355902, associated with significantly increased risks of both estrogen-receptor-positive (ER(+): odds ratio [OR] = 1.24, 95% confidence interval [CI] = 1.21-1.27, ptrend = 5.7 × 10(-44)) and estrogen-receptor-negative (ER(-): OR = 1.10, 95% CI = 1.05-1.15, ptrend = 3.0 × 10(-4)) tumors. After adjustment for rs62355902, we found evidence of association of a further 173 variants (iCHAV2) containing three subsets with a range of effects (the strongest was rs113317823 [pcond = 1.61 × 10(-5)]) and five variants composing iCHAV3 (lead rs11949391; ER(+): OR = 0.90, 95% CI = 0.87-0.93, pcond = 1.4 × 10(-4)). Twenty-six percent of the prioritized candidate variants coincided with four putative regulatory elements that interact with the MAP3K1 promoter through chromatin looping and affect MAP3K1 promoter activity. Functional analysis indicated that the cancer risk alleles of four candidates (rs74345699 and rs62355900 [iCHAV1], rs16886397 [iCHAV2a], and rs17432750 [iCHAV3]) increased MAP3K1 transcriptional activity. Chromatin immunoprecipitation analysis revealed diminished GATA3 binding to the minor (cancer-protective) allele of rs17432750, indicating a mechanism for its action. We propose that the cancer risk alleles act to increase MAP3K1 expression in vivo and might promote breast cancer cell survival.

  11. Fine-Scale Mapping of the 5q11.2 Breast Cancer Locus Reveals at Least Three Independent Risk Variants Regulating MAP3K1

    Science.gov (United States)

    Glubb, Dylan M.; Maranian, Mel J.; Michailidou, Kyriaki; Pooley, Karen A.; Meyer, Kerstin B.; Kar, Siddhartha; Carlebur, Saskia; O’Reilly, Martin; Betts, Joshua A.; Hillman, Kristine M.; Kaufmann, Susanne; Beesley, Jonathan; Canisius, Sander; Hopper, John L.; Southey, Melissa C.; Tsimiklis, Helen; Apicella, Carmel; Schmidt, Marjanka K.; Broeks, Annegien; Hogervorst, Frans B.; van der Schoot, C. Ellen; Muir, Kenneth; Lophatananon, Artitaya; Stewart-Brown, Sarah; Siriwanarangsan, Pornthep; Fasching, Peter A.; Ruebner, Matthias; Ekici, Arif B.; Beckmann, Matthias W.; Peto, Julian; dos-Santos-Silva, Isabel; Fletcher, Olivia; Johnson, Nichola; Pharoah, Paul D.P.; Bolla, Manjeet K.; Wang, Qin; Dennis, Joe; Sawyer, Elinor J.; Tomlinson, Ian; Kerin, Michael J.; Miller, Nicola; Burwinkel, Barbara; Marme, Frederik; Yang, Rongxi; Surowy, Harald; Guénel, Pascal; Truong, Thérèse; Menegaux, Florence; Sanchez, Marie; Bojesen, Stig E.; Nordestgaard, Børge G.; Nielsen, Sune F.; Flyger, Henrik; González-Neira, Anna; Benitez, Javier; Zamora, M. Pilar; Arias Perez, Jose Ignacio; Anton-Culver, Hoda; Neuhausen, Susan L.; Brenner, Hermann; Dieffenbach, Aida Karina; Arndt, Volker; Stegmaier, Christa; Meindl, Alfons; Schmutzler, Rita K.; Brauch, Hiltrud; Ko, Yon-Dschun; Brüning, Thomas; Nevanlinna, Heli; Muranen, Taru A.; Aittomäki, Kristiina; Blomqvist, Carl; Matsuo, Keitaro; Ito, Hidemi; Iwata, Hiroji; Tanaka, Hideo; Dörk, Thilo; Bogdanova, Natalia V.; Helbig, Sonja; Lindblom, Annika; Margolin, Sara; Mannermaa, Arto; Kataja, Vesa; Kosma, Veli-Matti; Hartikainen, Jaana M.; Wu, Anna H.; Tseng, Chiu-chen; Van Den Berg, David; Stram, Daniel O.; Lambrechts, Diether; Zhao, Hui; Weltens, Caroline; van Limbergen, Erik; Chang-Claude, Jenny; Flesch-Janys, Dieter; Rudolph, Anja; Seibold, Petra; Radice, Paolo; Peterlongo, Paolo; Barile, Monica; Capra, Fabio; Couch, Fergus J.; Olson, Janet E.; Hallberg, Emily; Vachon, Celine; Giles, Graham G.; Milne, Roger L.; McLean, Catriona; Haiman, Christopher A.; Henderson, Brian E.; Schumacher, Fredrick; Le Marchand, Loic; Simard, Jacques; Goldberg, Mark S.; Labrèche, France; Dumont, Martine; Teo, Soo Hwang; Yip, Cheng Har; See, Mee-Hoong; Cornes, Belinda; Cheng, Ching-Yu; Ikram, M. Kamran; Kristensen, Vessela; Zheng, Wei; Halverson, Sandra L.; Shrubsole, Martha; Long, Jirong; Winqvist, Robert; Pylkäs, Katri; Jukkola-Vuorinen, Arja; Kauppila, Saila; Andrulis, Irene L.; Knight, Julia A.; Glendon, Gord; Tchatchou, Sandrine; Devilee, Peter; Tollenaar, Robert A.E.M.; Seynaeve, Caroline; Van Asperen, Christi J.; García-Closas, Montserrat; Figueroa, Jonine; Chanock, Stephen J.; Lissowska, Jolanta; Czene, Kamila; Klevebring, Daniel; Darabi, Hatef; Eriksson, Mikael; Hooning, Maartje J.; Hollestelle, Antoinette; Martens, John W.M.; Collée, J. Margriet; Hall, Per; Li, Jingmei; Humphreys, Keith; Shu, Xiao-Ou; Lu, Wei; Gao, Yu-Tang; Cai, Hui; Cox, Angela; Cross, Simon S.; Reed, Malcolm W.R.; Blot, William; Signorello, Lisa B.; Cai, Qiuyin; Shah, Mitul; Ghoussaini, Maya; Kang, Daehee; Choi, Ji-Yeob; Park, Sue K.; Noh, Dong-Young; Hartman, Mikael; Miao, Hui; Lim, Wei Yen; Tang, Anthony; Hamann, Ute; Torres, Diana; Jakubowska, Anna; Lubinski, Jan; Jaworska, Katarzyna; Durda, Katarzyna; Sangrajrang, Suleeporn; Gaborieau, Valerie; Brennan, Paul; McKay, James; Olswold, Curtis; Slager, Susan; Toland, Amanda E.; Yannoukakos, Drakoulis; Shen, Chen-Yang; Wu, Pei-Ei; Yu, Jyh-Cherng; Hou, Ming-Feng; Swerdlow, Anthony; Ashworth, Alan; Orr, Nick; Jones, Michael; Pita, Guillermo; Alonso, M. Rosario; Álvarez, Nuria; Herrero, Daniel; Tessier, Daniel C.; Vincent, Daniel; Bacot, Francois; Luccarini, Craig; Baynes, Caroline; Ahmed, Shahana; Healey, Catherine S.; Brown, Melissa A.; Ponder, Bruce A.J.; Chenevix-Trench, Georgia; Thompson, Deborah J.; Edwards, Stacey L.; Easton, Douglas F.; Dunning, Alison M.; French, Juliet D.

    2015-01-01

    Genome-wide association studies (GWASs) have revealed SNP rs889312 on 5q11.2 to be associated with breast cancer risk in women of European ancestry. In an attempt to identify the biologically relevant variants, we analyzed 909 genetic variants across 5q11.2 in 103,991 breast cancer individuals and control individuals from 52 studies in the Breast Cancer Association Consortium. Multiple logistic regression analyses identified three independent risk signals: the strongest associations were with 15 correlated variants (iCHAV1), where the minor allele of the best candidate, rs62355902, associated with significantly increased risks of both estrogen-receptor-positive (ER+: odds ratio [OR] = 1.24, 95% confidence interval [CI] = 1.21–1.27, ptrend = 5.7 × 10−44) and estrogen-receptor-negative (ER−: OR = 1.10, 95% CI = 1.05–1.15, ptrend = 3.0 × 10−4) tumors. After adjustment for rs62355902, we found evidence of association of a further 173 variants (iCHAV2) containing three subsets with a range of effects (the strongest was rs113317823 [pcond = 1.61 × 10−5]) and five variants composing iCHAV3 (lead rs11949391; ER+: OR = 0.90, 95% CI = 0.87–0.93, pcond = 1.4 × 10−4). Twenty-six percent of the prioritized candidate variants coincided with four putative regulatory elements that interact with the MAP3K1 promoter through chromatin looping and affect MAP3K1 promoter activity. Functional analysis indicated that the cancer risk alleles of four candidates (rs74345699 and rs62355900 [iCHAV1], rs16886397 [iCHAV2a], and rs17432750 [iCHAV3]) increased MAP3K1 transcriptional activity. Chromatin immunoprecipitation analysis revealed diminished GATA3 binding to the minor (cancer-protective) allele of rs17432750, indicating a mechanism for its action. We propose that the cancer risk alleles act to increase MAP3K1 expression in vivo and might promote breast cancer cell survival. PMID:25529635

  12. Research and Development of P2P Worms

    Institute of Scientific and Technical Information of China (English)

    Li You; Zhi-Guang Qin

    2011-01-01

    With the development and the application of many popular peer-to-peer (P2P) systems such as eMule and BitTorrent,worms probably employ the features of these P2P networks to put them at risk.Some features,such as the local routing table and the application routing mechanism,are helpful to quickly distribute the P2P worms into the networks.This paper aims to give a comprehensive survey of P2P worms.The definition and the classification of P2P worms are discussed firstly.Then,the research and development of P2P worms, including experimental analysis,propagation modeling,and defensive approaches,are addressed and analyzed in detail.

  13. The 4p/sup 3/(/sup 2/P) ns, nd configurations of Se I

    Energy Technology Data Exchange (ETDEWEB)

    Mazzoni, M. (Florence Univ. (Italy). Dipt. di Astronomia e Scienza dello Spazio)

    1989-11-01

    The photoabsorption spectrum of Se I has been photographed in the 1100-900 A wavelength region, using a flash-pyrolisys system: About twenty lines were observed, most of them for the first time. With the support of Hartree-Fock calculations they have been identified and assigned to the 4p/sup 4/->4p/sup 3/ns/sup 3/P(n=7-14) and 4p/sup 4/->4p/sup 3/nd/sup 3/D (n=5-17) series, both converging on the limit 4p/sup 3/(/sup 2/P/sub 3/2/). (orig.).

  14. P2P Networking and Technology Enablers in Business Applications

    OpenAIRE

    Hariharan, Mahesh

    2006-01-01

    The usage of Peer to Peer Networks over the Internet has been growing by exponentially. Apartbfrom the hype surrounding P2P, it has remarkable ramifications on the way the Internet could be used. This is an area which is not explored as well as we would want to. This thesis examines the architectural differences in P2P networks and generic application domains where the principles of P2P are exploited. The usage of P2P in different business verticals and technology enablers that go along with ...

  15. P2P worm detection based on application identification

    Institute of Scientific and Technical Information of China (English)

    XIA Chunhe; SHI Yunping; LI Xiaojian; GAO Wei

    2007-01-01

    P2P worm exploits common vulnerabilities and spreads through peer-to-peer networks.Despite being recognized as a potential and deadly threat to the Internet recently,few relevant countermeasures are found in extant literature.Once it breaks out,a P2P worm could result in unpredictable losses.Based on propagation characteristics of the worm,this paper presents a detection method called PWD (P2P Worm Detection),which is designed based on application identification and unknown worm detection.Simulation result and LAN-environment experiment result both indicate that PWD is an effective method to detect and block P2P worms.

  16. Search by shortcuts in P2P scientific collaboration system

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    A P2P scientific collaboration is a P2P network whose members can share documents, co-compile papers and codes, and communicate with each other instantly. From the simulation experiment we found that P2P collaboration system is a power-law network with a tail between -2 and -3.We utilized the algorithm that searches by high-degree shortcuts to improve the scalability of p2p collaboration system. The experimental result shows that the algorithm works better than random walk algorithm.

  17. Relativistic CI calculations of spectroscopic data for the 2p{sup 6} and 2p{sup 5}3l configurations in Ne-like ions between Mg III and Kr XXVII

    Energy Technology Data Exchange (ETDEWEB)

    Jönsson, P., E-mail: per.jonsson@mah.se [School of Technology, Malmö University, 20506 Malmö (Sweden); Bengtsson, P.; Ekman, J.; Gustafsson, S.; Karlsson, L.B. [School of Technology, Malmö University, 20506 Malmö (Sweden); Gaigalas, G. [Department of Physics, Vilnius Pedagogical University, Studentu 39, LT-08106 Vilnius (Lithuania); Vilnius University, Institute of Theoretical Physics and Astronomy, A. Goštauto 12, LT-01108 Vilnius (Lithuania); Froese Fischer, C. [National Institute of Standards and Technology Gaithersburg, MD 20899-8420 (United States); Kato, D.; Murakami, I.; Sakaue, H.A. [National Institute for Fusion Science, 322-6 Oroshi-cho, Toki 509-5292 (Japan); Hara, H.; Watanabe, T. [National Astronomical Observatory of Japan, Mitaka, Tokyo 181-8588 (Japan); Nakamura, N. [The University of Electro-Communications, Chofu, Tokyo 182-8585 (Japan); Yamamoto, N. [Chubu University, Kasugai, Aichi 487-8501 (Japan)

    2014-01-15

    Energies, E1, M1, E2, M2 transition rates, oscillator strengths, and lifetimes from relativistic configuration interaction calculations are reported for the states of the 2p{sup 6}, 2p{sup 5}3s, 2p{sup 5}3p, and 2p{sup 5}3d, configurations in all Ne-like ions between Mg III and Kr XXVII. Core–valence and core–core correlation effects are accounted for through single and double excitations to increasing sets of active orbitals. The Breit interaction and leading quantum electrodynamic effects are included as perturbations. The results are compared with experiments and other recent benchmark calculations. In Mg III, Al IV, Si V, P VI, S VII, and Ar IX, for which experimental energies are known to high accuracy, the mean error in the calculated energies is only 0.011%.

  18. Association between alcoholism and the genetic polymorphisms of the GABAA receptor genes on chromosome 5q33-34 in Korean population.

    Science.gov (United States)

    Park, Chul-Soo; Park, So-Young; Lee, Chul-Soon; Sohn, Jin-Wook; Hahn, Gyu-Hee; Kim, Bong-Jo

    2006-06-01

    Family, twin, and adoption studies have demonstrated that genes play an important role in the development of alcoholism. We investigated the association between alcoholism and the genetic polymorphisms of the GABAA receptor genes on chromosome 5q33-34 in Korean population. The genotype of the GABAA receptor gene polymorphisms were determined by performing polymerase chain reaction genotyping for 172 normal controls and 162 male alcoholics who are hospitalized in alcoholism treatment institute. We found a significant association between the genetic polymorphisms of the GABAA alpha1 and GABAA alpha6 receptor gene and alcoholism. The GG genotype of the GABAA alpha1 receptor gene was associated with the onset age of alcoholism and alcohol withdrawal symptoms, and a high score on the Korean version of the ADS. However, there was no association between the genetic polymorphisms of the GABAA beta2 and gamma2 receptor gene and alcoholisms. Our finding suggest that genetic polymorphisms of the GABAA alpha1 and GABAA alpha6 receptor gene may be associated with the development of alcoholism and that the GG genotype of the GABAA alpha1 receptor gene play an important role in the development of the early onset and the severe type of alcoholism.

  19. Methods of Reprogramming to Induced Pluripotent Stem Cell Associated with Chromosomal Integrity and Delineation of a Chromosome 5q Candidate Region for Growth Advantage.

    Science.gov (United States)

    Sobol, Maria; Raykova, Doroteya; Cavelier, Lucia; Khalfallah, Ayda; Schuster, Jens; Dahl, Niklas

    2015-09-01

    Induced pluripotent stem cells (iPSCs) have brought great promises for disease modeling and cell-based therapies. One concern related to the use of reprogrammed somatic cells is the loss of genomic integrity and chromosome stability, a hallmark for cancer and many other human disorders. We investigated 16 human iPSC lines reprogrammed by nonintegrative Sendai virus (SeV) and another 16 iPSC lines generated by integrative lentivirus for genetic changes. At early passages we detected cytogenetic rearrangements in 44% (7/16) of iPSC lines generated by lentiviral integration whereas the corresponding figure was 6% (1/16) using SeV-based delivery. The rearrangements were numerical and/or structural with chromosomes 5 and 12 as the most frequently involved chromosomes. Three iPSC lines with chromosome 5 aberrations were derived from one and the same donor. We present in this study the aberrant karyotypes including a duplication of chromosome 5q13q33 that restricts a candidate region for growth advantage. Our results suggest that the use of integrative lentivirus confers a higher risk for cytogenetic abnormalities at early passages when compared to SeV-based reprogramming. In combination, our findings expand the knowledge on acquired cytogenetic aberrations in iPSC after reprogramming and during culture.

  20. The human granzyme A (HFSP, CTLA3) gene maps to 5q11-q12 and defines a new locus of the serine protease superfamily

    Energy Technology Data Exchange (ETDEWEB)

    Fink, T.M.; Lichter, P. (Institut fuer angewandte Tumorvirologie, Heidelberg (Germany)); Wekerle, H.; Zimmer, M.; Jenne, D.E. (Max-Planck-Institut fuer Psychiatrie, Planegg-Martinsried (Germany))

    1993-11-01

    Human granzyme A (HFSP, Hanukah factor serine protease; CTLA3, cytotoxic T-lymphocyte-associated serine esterase-3), a homodimeric, trypsin-like serine protease of 60 kDa found in granules of cytolytic T cells and natural killer cells, is implicated in lymphocyte-mediated target cell lysis. It contributes to DNA fragmentation in perforin (PRF1)-lysed target cells through an unknown mechanism. The authors have isolated a cosmid clone for the functional gene of human granzyme A and established its complete exon-intron map of 10 kb. Using an 11-kb subfragment of the cloned genomic DNA as a probe, they have identified the chromosomal position of human granzyme A on 5q11-q12. Thus, the human granzyme A gene falls into a region of homology between human chromosome 5 and mouse chromosome 13, band D, where the mouse granzyme A gene has been located previously. The granzyme A gene is not linked to known members of the large superfamily of serine proteases. 20 refs., 2 figs.

  1. Long-Term Response in a Patient with del(5q Myelodysplastic Syndrome Who Discontinued Lenalidomide and Obtained a Good Response and Tolerance to Rechallenge

    Directory of Open Access Journals (Sweden)

    Francesco Pisani

    2014-04-01

    Full Text Available Background: The introduction of the immunomodulatory drug lenalidomide has revolutionized the treatment of patients with myelodysplastic syndromes (MDS and deletion of the long arm of chromosome 5. Treatment with lenalidomide results in transfusion independence in the majority of patients, but some questions remain unresolved, among them the duration of treatment. Moreover, a number of unexpected long-term remissions in patients who stopped lenalidomide for various reasons have been observed. Case Report: We report the case of a 60-year-old Caucasian male with deletion of the long arm of chromosome 5 and International Prognostic Scoring System (IPSS-defined low-risk MDS who was treated with lenalidomide, achieving complete cytogenetic remission and erythroid response. After tapering off and interrupting the treatment, the patient relapsed and showed a new response by lenalidomide retreatment. Six years after the initial treatment, we registered a durable erythroid long-term response and good tolerance, but there was no evidence of a very profound cytogenetic response compared to using lenalidomide as a first-line treatment. Cytogenetic and fluorescence in situ hybridization together with hemoglobin level, mean corpuscular volume (MCV and vitamin B12 level helped us to monitor the patient response; during the various phases of lenalidomide treatment, MCV and vitamin B12 normalization correlated with good response. Conclusion: Lenalidomide interruption and rechallenge in some 5q- MDS patients, with low risk according to the IPSS, is safe and feasible but does not result in a profound cytogenetic response.

  2. Research of P2P SIP technology%P2P SIP技术的研究

    Institute of Scientific and Technical Information of China (English)

    隋晋光; 鲁士文

    2007-01-01

    在阐述P2P和SIP技术的基础上,引出了一种二者融合的新技术--P2P SIP,提出了采用P2P SIP技术系统的体系结构、工作方式,并且对P2P SIP技术的安全性问题进行了分析.

  3. Isotope shifts of the 2$p_{3/2}$-2$p_{1/2}$ transition in B-like ions

    CERN Document Server

    Zubova, N A; Tupitsyn, I I; Shabaev, V M; Kozhedub, Y S; Plunien, G; Brandau, C; Stohlker, Th

    2016-01-01

    Isotope shifts of the 2$p_{3/2}$-2$p_{1/2}$ transition in B-like ions are evaluated for a wide range of the nuclear charge number: Z=8-92. The calculations of the relativistic nuclear recoil and nuclear size effects are performed using a large scale configuration-interaction Dirac-Fock-Sturm method. The corresponding QED corrections are also taken into account. The results of the calculations are compared with the theoretical values obtained with other methods. The accuracy of the isotope shifts of the 2$p_{3/2}$-2$p_{1/2}$ transition in B-like ions is significantly improved.

  4. Isotope shifts of the 2 p3 /2-2 p1 /2 transition in B-like ions

    Science.gov (United States)

    Zubova, N. A.; Malyshev, A. V.; Tupitsyn, I. I.; Shabaev, V. M.; Kozhedub, Y. S.; Plunien, G.; Brandau, C.; Stöhlker, Th.

    2016-05-01

    Isotope shifts of the 2 p3 /2-2 p1 /2 transition in B-like ions are evaluated for a wide range of the nuclear charge number: Z =8 -92 . The calculations of the relativistic nuclear recoil and nuclear size effects are performed using a large-scale configuration-interaction Dirac-Fock-Sturm method. The corresponding QED corrections are also taken into account. The results of the calculations are compared with the theoretical values obtained with other methods. The accuracy of the isotope shifts of the 2 p3 /2-2 p1 /2 transition in B-like ions is significantly improved.

  5. Magnetocrystalline anisotropy and the magnetocaloric effect in Fe2P

    NARCIS (Netherlands)

    Caron, L.; Hudl, M.; Höglin, V.; Dung, N.H.; Gomez, C.P.; Sahlberg, M.; Brück, E.; Andersson, Y.; Nordblad, P.

    2013-01-01

    Magnetic and magnetocaloric properties of high-purity, giant magnetocaloric polycrystalline and single-crystalline Fe2P are investigated. Fe2P displays a moderate magnetic entropy change, which spans over 70 K and the presence of strong magnetization anisotropy proves this system is not fully itiner

  6. NLO NRQCD disfavors the interpretation of X(3872) as {chi}{sub c1}(2P)

    Energy Technology Data Exchange (ETDEWEB)

    Butenschoen, Mathias [Wien Univ. (Austria). Fakultaet fuer Physik; He, Zhi-Guo; Kniehl, Bernd A. [Hamburg Univ. (Germany). 2. Inst. fuer Theoretische Physik

    2013-03-15

    We study {chi}{sub c1}(2P) inclusive hadroproduction at next-to-leading order (NLO) within the factorization formalism of nonrelativistic quantum chromodynamics (NRQCD), including both the color-singlet {sup 3}P{sub 1}{sup [1]} and color-octet {sup 3}S{sub 1}{sup [8]} c anti c Fock states. Assuming the recently discovered X(3872) hadron to be the 2P (1{sup ++}) charmonium state, we perform a fit to the cross sections measured by the CDF, CMS, and LHCb Collaborations. We either obtain an unacceptably high value of {chi}{sup 2} or a value of vertical stroke R{sub 2P}{sup '}(0) vertical stroke incompatible with well-established potential models. We thus conclude that NLO NRQCD is incompatible with the hypothesis X(3872){identical_to}{chi}{sub c1}(2P).

  7. Superconductivity in noncentrosymmetric A g2P d3S

    Science.gov (United States)

    Yoshida, H.; Okabe, H.; Matsushita, Y.; Isobe, M.; Takayama-Muromachi, E.

    2017-05-01

    We have successfully synthesized the single crystal of A g2P d3S , which exhibits superconductivity with the transition temperature of Tc=2.25 K . A g2P d3S crystallizes in the space group P 4132 with the filled β -Mn structure, which has no inversion symmetry. The value of the Ginzburg-Landau parameter κGL indicates that A g2P d3S is a type-II superconductor. Δ C (Tc) /γnTc=1.50 and 2 Δ /kBTc=3.48 from the heat-capacity analyses indicate that A g2P d3S is a weak-coupling Bardeen-Cooper-Schrieffer (BCS) superconductor with an isotropic superconducting gap. On the other hand, the violation of the Werthamer-Helfand-Hohenberg curve in the H -T phase diagram implies A g2P d3S is not a typical BCS superconductor.

  8. Hasil In Silico Senyawa Z12501572, Z00321025, SCB5631028 dan SCB13970547 dibandingkan Turunan Zerumbon terhadap Human Liver Glycogen Phosphorylase (1l5Q sebagai Antidiabetes

    Directory of Open Access Journals (Sweden)

    Fitri Kusvila Aziz

    2016-12-01

    Full Text Available Abstrak Human Liver Glycogen Phosphorylase (HLGP, suatu katalis glikogen yang mengontrol pelepasan glukosa-1-fosfat glikogen dari hati. Enzim ini mempunyai peran sentral dalam luaran glukosa hati sehingga menjadi target obat antidiabetik. Kajian docking dilakukan pada komputer dengan prosesor Intel Pentium, RAM 1 GB dan Windows 7. Ligan yang digunakan adalah senyawa obat (Z12501572, Z00321025, SCB5631028 dan SCB13970547, dataset pembanding aktif glycogen phosphorylase outer dimer site (PYGL-out dan decoysdari www.dekois.com dan turunan zerumbon. Protein dipisahkan dari ligan nativ dan semua ligan beserta protein dikonversi menggunakan PyRx. Visualisasi interaksi ligan-protein dihasilkan dengan program Protein-Ligand Interaction Profiler (PLIP dan PyMOL. Senyawa ZER11 memiliki binding energy terbaik, yaitu -7.11 kkal/mol (untuk metode LGA dan GA dan -4.08 kkal/mol untuk metode SA. Nilai binding energy tersebut lebih rendah dari pada nilai untuk ligan native dan satu dari keempat senyawa obat, terlebih jika dibandingkan dengan bindingaffinity dari dataset dan decoys. Interaksi ligan-protein pada ketiga metode tersebut ditemukan sangat bervariasi. Hal berbeda terjadi untuk metode Vina, bindingenergy ZER11 (-9.9 kkal/mol lebih baik dibandingkan dengan ligan native dan keempat senyawa obat. Senyawa ZER11 memiliki residu interaksi yang sama dengan ligan native pada TRP67 dan LYS191 untuk metode Vina. Kata kunci: PDBID-1L5Q, AutoDock, docking molekuler, vina, antidiabetes   Abstract Human Liver Glycogen Phosphorylase (HLGP can catalyze glycogen and control the release of glucose-1-phosphate of glycogen from the liver. This enzyme has a central role in output rule of liver glucose as it can be used as an antidiabetic drug targets. Docking studies were carried out on PC with Intel Pentium, 1 GB RAM, in environment of Windows 7. Ligands used are drug compounds (Z12501572, Z00321025, SCB5631028 and SCB13970547, the active dataset comparator

  9. Lenalidomide for the Treatment of Low- or Intermediate-1-Risk Myelodysplastic Syndromes Associated with Deletion 5q Cytogenetic Abnormality: An Evidence Review of the NICE Submission from Celgene.

    Science.gov (United States)

    Blommestein, Hedwig M; Armstrong, Nigel; Ryder, Steve; Deshpande, Sohan; Worthy, Gill; Noake, Caro; Riemsma, Rob; Kleijnen, Jos; Severens, Johan L; Al, Maiwenn J

    2016-01-01

    The National Institute for Health and Care Excellence (NICE) invited the manufacturer of lenalidomide (Celgene) to submit evidence of the clinical and cost effectiveness of the drug for treating adults with myelodysplastic syndromes (MDS) associated with deletion 5q cytogenetic abnormality, as part of the Institute's single technology appraisal (STA) process. Kleijnen Systematic Reviews Ltd (KSR), in collaboration with Erasmus University Rotterdam, was commissioned to act as the Evidence Review Group (ERG). This paper describes the company's submission, the ERG review, and the NICE's subsequent decisions. The ERG reviewed the evidence for clinical and cost effectiveness of the technology, as submitted by the manufacturer to the NICE. The ERG searched for relevant additional evidence and validated the manufacturer's decision analytic model to examine the robustness of the cost-effectiveness results. Clinical effectiveness was obtained from a three-arm, European, randomized, phase III trial among red blood cell (RBC) transfusion-dependent patients with low-/intermediate-1-risk del5q31 MDS. The primary endpoint was RBC independence for ≥26 weeks, and was reached by a higher proportion of patients in the lenalidomide 10 and 5 mg groups compared with placebo (56.1 and 42.6 vs 5.9 %, respectively; both p < 0.001). The option of dose adjustments after 16 weeks due to dose-limiting toxicities or lack of response made long-term effectiveness estimates unreliable, e.g. overall survival (OS). The de novo model of the manufacturer included a Markov state-transition cost-utility model implemented in Microsoft Excel. The base-case incremental cost-effectiveness ratio (ICER) of the manufacturer was £56,965. The ERG assessment indicated that the modeling structure represented the course of the disease; however, a few errors were identified and some of the input parameters were challenged. In response to the appraisal documentation, the company revised the economic model

  10. Queries mining for efficient routing in P2P communities

    CERN Document Server

    Ismail, Anis; Durand, Nicolas; Nachouki, Gilles; Hajjar, Mohammad

    2011-01-01

    Peer-to-peer (P2P) computing is currently attracting enormous attention. In P2P systems a very large number of autonomous computing nodes (the peers) pool together their resources and rely on each other for data and services. Peer-to-peer (P2P) Data-sharing systems now generate a significant portion of Internet traffic. Examples include P2P systems for network storage, web caching, searching and indexing of relevant documents and distributed network-threat analysis. Requirements for widely distributed information systems supporting virtual organizations have given rise to a new category of P2P systems called schema-based. In such systems each peer exposes its own schema and the main objective is the efficient search across the P2P network by processing each incoming query without overly consuming bandwidth. The usability of these systems depends on effective techniques to find and retrieve data; however, efficient and effective routing of content-based queries is a challenging problem in P2P networks. This wo...

  11. A Narrow and Highly Significant Linkage Signal for Severe Bipolar Disorder in the Chromosome 5q33 Region in Latin American Pedigrees

    Science.gov (United States)

    Jasinska, A.J.; Service, S.; Jawaheer, D.; DeYoung, J.; Levinson, M.; Zhang, Z.; Kremeyer, B.; Muller, H.; Aldana, I.; Garcia, J.; Restrepo, G.; Lopez, C.; Palacio, C.; Duque, C.; Parra, M.; Vega, J.; Ortiz, D.; Bedoya, G.; Mathews, C.; Davanzo, P.; Fournier, E.; Bejarano, J.; Ramirez, M.; Ortiz, C. Araya; Araya, X.; Molina, J.; Sabatti, C.; Reus, V.; Ospina, J.; Macaya, G.; Ruiz-Linares, A.; Freimer, N.B.

    2016-01-01

    We previously reported linkage of bipolar disorder to 5q33-q34 in families from two closely related population isolates, the Central Valley of Costa Rica (CVCR) and Antioquia, Colombia (CO). Here we present follow up results from fine-scale mapping in large CVCR and CO families segregating severe bipolar disorder, BP-I, and in 343 population trios/duos from CVCR and CO. Employing densely spaced SNPs to fine map the prior linkage peak region increases linkage evidence and clarifies the position of the putative BP-I locus. We performed two-point linkage analysis with 1134 SNPs in an approximately 9 Mb region between markers D5S410 and D5S422. Combining pedigrees from CVCR and CO yields a LOD score of 4.9 at SNP rs10035961. Two other SNPs (rs7721142 and rs1422795) within the same 94 kb region also displayed LOD scores greater than 4. This linkage peak coincides with our prior microsatellite results and suggests a narrowed BP-I susceptibility regions in these families. To investigate if the locus implicated in the familial form of BP-I also contributes to disease risk in the population, we followed up the family results with association analysis in duo and trio samples, obtaining signals within 2 Mb of the peak linkage signal in the pedigrees; rs12523547 and rs267015 (P = 0.00004 and 0.00016, respectively) in the CO sample and rs244960 in the CVCR sample and the combined sample, with P = 0.00032 and 0.00016, respectively. It remains unclear whether these association results reflect the same locus contributing to BP susceptibility within the extended pedigrees. PMID:19319892

  12. A chromosome 5q31.1 locus associates with tuberculin skin test reactivity in HIV-positive individuals from tuberculosis hyper-endemic regions in east Africa.

    Science.gov (United States)

    Sobota, Rafal S; Stein, Catherine M; Kodaman, Nuri; Maro, Isaac; Wieland-Alter, Wendy; Igo, Robert P; Magohe, Albert; Malone, LaShaunda L; Chervenak, Keith; Hall, Noemi B; Matee, Mecky; Mayanja-Kizza, Harriet; Joloba, Moses; Moore, Jason H; Scott, William K; Lahey, Timothy; Boom, W Henry; von Reyn, C Fordham; Williams, Scott M; Sirugo, Giorgio

    2017-06-01

    One in three people has been infected with Mycobacterium tuberculosis (MTB), and the risk for MTB infection in HIV-infected individuals is even higher. We hypothesized that HIV-positive individuals living in tuberculosis-endemic regions who do not get infected by Mycobacterium tuberculosis are genetically resistant. Using an "experiment of nature" design that proved successful in our previous work, we performed a genome-wide association study of tuberculin skin test positivity using 469 HIV-positive patients from prospective study cohorts of tuberculosis from Tanzania and Uganda to identify genetic loci associated with MTB infection in the context of HIV-infection. Among these individuals, 244 tested were tuberculin skin test (TST) positive either at enrollment or during the >8 year follow up, while 225 were not. We identified a genome-wide significant association between a dominant model of rs877356 and binary TST status in the combined cohort (Odds ratio = 0.2671, p = 1.22x10-8). Association was replicated with similar significance when examining TST induration as a continuous trait. The variant lies in the 5q31.1 region, 57kb downstream from IL9. Two-locus analyses of association of variants near rs877356 showed a haplotype comprised of rs877356 and an IL9 missense variant, rs2069885, had the most significant association (p = 1.59x10-12). We also replicated previously linked loci on chromosomes 2, 5, and 11. IL9 is a cytokine produced by mast cells and TH2 cells during inflammatory responses, providing a possible link between airway inflammation and protection from MTB infection. Our results indicate that studying uninfected, HIV-positive participants with extensive exposure increases the power to detect associations in complex infectious disease.

  13. Distributed Frequent Item Sets Mining over P2P Networks

    OpenAIRE

    Zahra Farzanyar; Mohammadreza Kangavari

    2015-01-01

    Data intensive peer-to-peer (P2P) networks are becoming increasingly popular in applications like social networking, file sharing networks, etc. Data mining in such P2P environments is the new generation of advanced P2P applications. Unfortunately, most of the existing data mining algorithms do not fit well in such environments since they require data that can be accessed in its entirety. It also is not easy due to the requirements of online transactional data streams. In this paper, we have ...

  14. Increasing Structured P2P Protocol Resilience to Localized Attacks

    OpenAIRE

    Germanus, Daniel

    2015-01-01

    The Peer-to-Peer (P2P) computing model has been applied to many application fields over the last decade. P2P protocols made their way from infamous - and frequently illicit - file sharing applications towards serious applications, e.g., in entertainment, audio/video conferencing, or critical applications like smart grid, Car-2-Car communication, or Machine-to-Machine communication. Some of the reasons for that are P2P's decentralized design that inherently provides for fault tolerance to non-...

  15. Code wars 10 years of P2P software litigation

    CERN Document Server

    Giblin, Rebecca

    2011-01-01

    Code Wars recounts the legal and technological history of the first decade of the P2P file sharing era, focusing on the innovative and anarchic ways in which P2P technologies evolved in response to decisions reached by courts with regard to their predecessors. With reference to US, UK, Canadian and Australian secondary liability regimes, this insightful book develops a compelling new theory to explain why a decade of ostensibly successful litigation failed to reduce the number, variety or availability of P2P file sharing applications - and highlights ways the law might need to change if it is

  16. Comparing Pedophile Activity in Different P2P Systems

    OpenAIRE

    Raphaël Fournier; Thibault Cholez; Matthieu Latapy; Isabelle Chrisment; Clémence Magnien; Olivier Festor; Ivan Daniloff

    2014-01-01

    International audience; Peer-to-peer (P2P) systems are widely used to exchange content over the Internet. Knowledge of pedophile activity in such networks remains limited, despite having important social consequences. Moreover, though there are different P2P systems in use, previous academic works on this topic focused on one system at a time and their results are not directly comparable. We design a methodology for comparing KAD and eDonkey, two P2P systems among the most prominent ones and ...

  17. Data Sharing in DHT Based P2P Systems

    Science.gov (United States)

    Roncancio, Claudia; Del Pilar Villamil, María; Labbé, Cyril; Serrano-Alvarado, Patricia

    The evolution of peer-to-peer (P2P) systems triggered the building of large scale distributed applications. The main application domain is data sharing across a very large number of highly autonomous participants. Building such data sharing systems is particularly challenging because of the “extreme” characteristics of P2P infrastructures: massive distribution, high churn rate, no global control, potentially untrusted participants... This article focuses on declarative querying support, query optimization and data privacy on a major class of P2P systems, that based on Distributed Hash Table (P2P DHT). The usual approaches and the algorithms used by classic distributed systems and databases for providing data privacy and querying services are not well suited to P2P DHT systems. A considerable amount of work was required to adapt them for the new challenges such systems present. This paper describes the most important solutions found. It also identifies important future research trends in data management in P2P DHT systems.

  18. P2P Data Management in Mobile Wireless Sensor Network

    Directory of Open Access Journals (Sweden)

    Nida Sahar Sayeda

    2013-04-01

    Full Text Available The rapid growth in wireless technologies has made wireless communication an important source for transporting data across different domains. In the same way, there are possibilities of many potential applications that can be deployed using WSNs (Wireless Sensor Networks. However, very limited applications are deployed in real life due to the uncertainty and dynamics of the environment and scare resources. This makes data management in WSN a challenging area to find an approach that suits its characteristics. Currently, the trend is to find efficient data management schemes using evolving technologies, i.e. P2P (Peer-to-Peer systems. Many P2P approaches have been applied in WSNs to carry out the data management due to similarities between WSN and P2P. With the similarities, there are differences too that makes P2P protocols inefficient in WSNs. Furthermore, to increase the efficiency and to exploit the delay tolerant nature of WSNs, where ever possible, the mobile WSNs are gaining importance. Thus, creating a three dimensional problem space to consider, i.e. mobility, WSNs and P2P. In this paper, an efficient algorithm is proposed for data management using P2P techniques for mobile WSNs. The real world implementation and deployment of proposed algorithm is also presented

  19. Network Awareness in P2P-TV Applications

    Science.gov (United States)

    Traverso, Stefano; Leonardi, Emilio; Mellia, Marco; Meo, Michela

    The increasing popularity of applications for video-streaming based on P2P paradigm (P2P-TV) is raising the interest of both broadcasters and network operators. The former see a promising technology to reduce the cost of streaming content over the Internet, while offering a world-wide service. The latter instead fear that the traffic offered by these applications can grow without control, affecting other services, and possibly causing network congestion and collapse. The “Network-Aware P2P-TV Application over Wise Networks” FP7 project aims at studying and developing a novel P2P-TV application offering the chance to broadcast high definition video to broadcasters and to carefully manage the traffic offered by peers to the network, therefore avoiding worries to Internet providers about network overload. In such context, we design a simulator to evaluate performance of different P2P-TV solutions, to compare them both considering end-users’ and network providers’ perspectives, such as quality of service perceived by subscribers and link utilization. In this paper, we provide some results that show how effective can be a network aware P2P-TV system.

  20. Improving P2P live-content delivery using SVC

    Science.gov (United States)

    Schierl, T.; Sánchez, Y.; Hellge, C.; Wiegand, T.

    2010-07-01

    P2P content delivery techniques for video transmission have become of high interest in the last years. With the involvement of client into the delivery process, P2P approaches can significantly reduce the load and cost on servers, especially for popular services. However, previous studies have already pointed out the unreliability of P2P-based live streaming approaches due to peer churn, where peers may ungracefully leave the P2P infrastructure, typically an overlay networks. Peers ungracefully leaving the system cause connection losses in the overlay, which require repair operations. During such repair operations, which typically take a few roundtrip times, no data is received from the lost connection. While taking low delay for fast-channel tune-in into account as a key feature for broadcast-like streaming applications, the P2P live streaming approach can only rely on a certain media pre-buffer during such repair operations. In this paper, multi-tree based Application Layer Multicast as a P2P overlay technique for live streaming is considered. The use of Flow Forwarding (FF), a.k.a. Retransmission, or Forward Error Correction (FEC) in combination with Scalable video Coding (SVC) for concealment during overlay repair operations is shown. Furthermore the benefits of using SVC over the use of AVC single layer transmission are presented.

  1. Characterization of P2P IPTV Traffic: Scaling Analysis

    CERN Document Server

    Silverston, Thomas; Salamatian, Kave

    2007-01-01

    P2P IPTV applications arise on the Internet and will be massively used in the future. It is expected that P2P IPTV will contribute to increase the overall Internet traffic. In this context, it is important to measure the impact of P2P IPTV on the networks and to characterize this traffic. During the 2006 FIFA World Cup, we performed an extensive measurement campaign. We measured network traffic generated by broadcasting soccer games by the most popular P2P IPTV applications, namely PPLive, PPStream, SOPCast and TVAnts. From the collected data, we characterized the P2P IPTV traffic structure at different time scales. To the best of our knowledge, this is the first work, which presents a complete multiscale analysis of the P2P IPTV traffic. Our observations show that the network traffic has not the same scale behavior whether the applications use TCP or UDP. For all the applications, the download traffic is different from the upload traffic and the signaling traffic has an impact on the download traffic.

  2. Health-related quality of life outcomes of lenalidomide in transfusion-dependent patients with Low- or Intermediate-1-risk myelodysplastic syndromes with a chromosome 5q deletion: results from a randomized clinical trial.

    NARCIS (Netherlands)

    Revicki, D.A.; Brandenburg, N.A.; Muus, P.; Yu, R.; Knight, R.; Fenaux, P.

    2013-01-01

    We report health-related quality of life (HRQL) outcomes assessed using the Functional Assessment of Cancer Therapy-Anemia (FACT-An) among 167 RBC transfusion-dependent patients with IPSS Low-/Intermediate-1-risk del5q31 MDS treated with lenalidomide versus placebo in a randomized phase 3 clinical t

  3. Outcomes in RBC transfusion-dependent patients with Low-/Intermediate-1-risk myelodysplastic syndromes with isolated deletion 5q treated with lenalidomide: A subset analysis from the MDS-004 study

    NARCIS (Netherlands)

    A. Giagounidis (Aristoteles); G.J. Mufti (Ghulam); M. Mittelman (Moshe); G. Sanz (Guillermo); U. Platzbecker (Uwe); P. Muus (P.); D. Selleslag; O. Beyne-Rauzy (Odile); P.A.W. te Boekhorst (Peter); C. del Cañizo (Consuelo); A. Guerci-Bresler (Agnes); L. Nilsson (Lars); M. Lübbert (Michael); B. Quesnel (Bruno); A. Ganser (Arnold); D. Bowen (David); B. Schlegelberger (Brigitte); G. Göhring (Gudrun); T. Fu (Tommy); B. Benettaib (Bouchra); E. Hellström-Lindberg (Eva); P. Fenaux (Pierre)

    2014-01-01

    textabstractObjective: A subset analysis of the randomised, phase 3, MDS-004 study to evaluate outcomes in patients with International Prognostic Scoring System (IPSS)-defined Low-/Intermediate (Int)-1-risk myelodysplastic syndromes (MDS) with isolated del(5q). Methods: Patients received lenalidomid

  4. Why X(3915) is so narrow as a $\\chi_{c0}(2P)$ state

    CERN Document Server

    Jiang, Yue; Wang, Tianhong; Ju, Wan-Li

    2013-01-01

    New resonance X(3915) was identified as the charmonium $\\chi_{c0}(2P)$ by BABAR Collaboration, but there seems still open question of this assignment: why its full width is so narrow? To answer this question, we calculate the Okubo-Zweig-Iizuka (OZI) allowed strong decays $X(3915)\\to D \\bar D$, where X(3915) is assigned as a $\\chi_{c0}(2P)$ state, and estimate its full width in the cooperating framework of $^3/!P_0^{}$ model and the Bethe-Salpeter (BS) method using the Mandelstam Formalism, during which non-perturbative QCD effects of the hadronic matrix elements are well considered by overlapping integral over the relativistic Salpeter wave functions of the initial and finial states. We find the node structure of $\\chi_{c0}(2P)$ wave function resulting in the narrow width of X(3915) and show the dependence of the decay width on the variation of the initial mass of X(3915). We point out that the rate of $\\frac{\\Gamma(X(3915)\\to D^+ D^-)}{\\Gamma(X(3915)\\to D^0 \\bar D^0)}$ is crucial to confirm whether X(3915) ...

  5. Autoionization study of the Argon 2p satellites excited near the argon 2s threshold

    Energy Technology Data Exchange (ETDEWEB)

    Wang, H.; Glans, P.; Hemmers, O. [Univ. of Nevada, Las Vegas, NV (United States)] [and others

    1997-04-01

    The dynamics of near-threshold photoionization is a complex phenomenon in which the many-electron character of the wavefunctions plays an important role. According to generalized time-independent resonant scattering theory, the transition matrix element from an initial state to a final state is the summation of the amplitudes of direct photoionization and an indirect term in which intermediate states are involved and the resonant behavior is embedded. Studies of the interference effects of intermediate states have been explored in the cases where the direct term is negligible. In the present work, electron time-of-flight spectra of the Ar 2p satellites were measured at two angles (magic and 0{degrees}) in the dipole plane with the exciting photon energy tuned in the vicinity of the Ar 2s threshold. For excitation far below or above the 2s threshold, the 2p satellites spectrum is dominated by 3p to np shakeup contributions upon the ionization of a 2p electron.

  6. A sib-pair analysis study of 15 candidate genes in French families with morbid obesity: indication for linkage with islet 1 locus on chromosome 5q.

    Science.gov (United States)

    Clément, K; Dina, C; Basdevant, A; Chastang, N; Pelloux, V; Lahlou, N; Berlan, M; Langin, D; Guy-Grand, B; Froguel, P

    1999-02-01

    As part of an ongoing search for susceptibility genes in obese families, we performed linkage analyses in 101 French families between qualitative and quantitative traits related to morbid obesity and polymorphisms located in or near 15 candidate genes whose products are involved in body weight regulation. These included cholecystokinin A and B receptors (CCK-AR and CCK-BR), glucagon-like peptide 1 receptor (GLP-1R), the LIM/homeodomain islet-1 gene (Isl-1), the caudal-type homeodomain 3 (CDX-3), the uncoupling protein 1 (UCP-1), the beta3-adrenoceptor (beta3-AR), the fatty acid-binding protein 2 (FABP-2), the hormone-sensitive lipase (HSL), the lipoprotein lipase (LPL), the apoprotein-C2 (apo-C2), the insulin receptor substrate-1 (IRS-1), the peroxisome proliferator-activated receptor-gamma (PPAR-gamma), tumor necrosis factor-alpha (TNF-alpha), and the liver carnitine palmitoyltransferase-1 (CPT-1). Phenotypes related to obesity such as BMI, adult life body weight gain, fasting leptin, insulin, fasting glycerol, and free fatty acids were used for nonparametric sib-pair analyses. A weak indication for linkage was obtained between the Isl-1 locus and obesity status defined by a z score over one SD of BMI (n = 226 sib pairs, pi = 0.54 +/- 0.02, P = 0.03). Moreover, a suggestive indication for linkage was found between the Isl-1 locus and BMI and leptin values (P = 0.001 and 0.0003, respectively) and leptin adjusted for BMI (P = 0.0001). Multipoint analyses for leptin trait with Isl-1 and two flanking markers (D5S418 and D5S407) showed that the logarithm of odds (LOD) score is 1.73, coinciding with the Isl-1 locus. Although marginally positive indications for linkage in subgroups of families were found with IRS-1, CPT-1, and HSL loci, our data suggested that these genes are not major contributors to obesity. Whether an obesity susceptibility gene (Isl-1 itself or another nearby gene) lies on chromosome 5q should be determined by further analyses.

  7. Four novel Loci (19q13, 6q24, 12q24, and 5q14 influence the microcirculation in vivo.

    Directory of Open Access Journals (Sweden)

    M Kamran Ikram

    2010-10-01

    Full Text Available There is increasing evidence that the microcirculation plays an important role in the pathogenesis of cardiovascular diseases. Changes in retinal vascular caliber reflect early microvascular disease and predict incident cardiovascular events. We performed a genome-wide association study to identify genetic variants associated with retinal vascular caliber. We analyzed data from four population-based discovery cohorts with 15,358 unrelated Caucasian individuals, who are members of the Cohort for Heart and Aging Research in Genomic Epidemiology (CHARGE consortium, and replicated findings in four independent Caucasian cohorts (n  =  6,652. All participants had retinal photography and retinal arteriolar and venular caliber measured from computer software. In the discovery cohorts, 179 single nucleotide polymorphisms (SNP spread across five loci were significantly associated (p<5.0×10(-8 with retinal venular caliber, but none showed association with arteriolar caliber. Collectively, these five loci explain 1.0%-3.2% of the variation in retinal venular caliber. Four out of these five loci were confirmed in independent replication samples. In the combined analyses, the top SNPs at each locus were: rs2287921 (19q13; p  =  1.61×10(-25, within the RASIP1 locus, rs225717 (6q24; p = 1.25×10(-16, adjacent to the VTA1 and NMBR loci, rs10774625 (12q24; p  =  2.15×10(-13, in the region of ATXN2,SH2B3 and PTPN11 loci, and rs17421627 (5q14; p = 7.32×10(-16, adjacent to the MEF2C locus. In two independent samples, locus 12q24 was also associated with coronary heart disease and hypertension. Our population-based genome-wide association study demonstrates four novel loci associated with retinal venular caliber, an endophenotype of the microcirculation associated with clinical cardiovascular disease. These data provide further insights into the contribution and biological mechanisms of microcirculatory changes that underlie cardiovascular

  8. Determinants of Default in P2P Lending.

    Science.gov (United States)

    Serrano-Cinca, Carlos; Gutiérrez-Nieto, Begoña; López-Palacios, Luz

    2015-01-01

    This paper studies P2P lending and the factors explaining loan default. This is an important issue because in P2P lending individual investors bear the credit risk, instead of financial institutions, which are experts in dealing with this risk. P2P lenders suffer a severe problem of information asymmetry, because they are at a disadvantage facing the borrower. For this reason, P2P lending sites provide potential lenders with information about borrowers and their loan purpose. They also assign a grade to each loan. The empirical study is based on loans' data collected from Lending Club (N = 24,449) from 2008 to 2014 that are first analyzed by using univariate means tests and survival analysis. Factors explaining default are loan purpose, annual income, current housing situation, credit history and indebtedness. Secondly, a logistic regression model is developed to predict defaults. The grade assigned by the P2P lending site is the most predictive factor of default, but the accuracy of the model is improved by adding other information, especially the borrower's debt level.

  9. Determinants of Default in P2P Lending.

    Directory of Open Access Journals (Sweden)

    Carlos Serrano-Cinca

    Full Text Available This paper studies P2P lending and the factors explaining loan default. This is an important issue because in P2P lending individual investors bear the credit risk, instead of financial institutions, which are experts in dealing with this risk. P2P lenders suffer a severe problem of information asymmetry, because they are at a disadvantage facing the borrower. For this reason, P2P lending sites provide potential lenders with information about borrowers and their loan purpose. They also assign a grade to each loan. The empirical study is based on loans' data collected from Lending Club (N = 24,449 from 2008 to 2014 that are first analyzed by using univariate means tests and survival analysis. Factors explaining default are loan purpose, annual income, current housing situation, credit history and indebtedness. Secondly, a logistic regression model is developed to predict defaults. The grade assigned by the P2P lending site is the most predictive factor of default, but the accuracy of the model is improved by adding other information, especially the borrower's debt level.

  10. Determinants of Default in P2P Lending

    Science.gov (United States)

    2015-01-01

    This paper studies P2P lending and the factors explaining loan default. This is an important issue because in P2P lending individual investors bear the credit risk, instead of financial institutions, which are experts in dealing with this risk. P2P lenders suffer a severe problem of information asymmetry, because they are at a disadvantage facing the borrower. For this reason, P2P lending sites provide potential lenders with information about borrowers and their loan purpose. They also assign a grade to each loan. The empirical study is based on loans’ data collected from Lending Club (N = 24,449) from 2008 to 2014 that are first analyzed by using univariate means tests and survival analysis. Factors explaining default are loan purpose, annual income, current housing situation, credit history and indebtedness. Secondly, a logistic regression model is developed to predict defaults. The grade assigned by the P2P lending site is the most predictive factor of default, but the accuracy of the model is improved by adding other information, especially the borrower’s debt level. PMID:26425854

  11. Managing Linguistic Data Summaries in Advanced P2P Applications

    Science.gov (United States)

    Hayek, Rabab; Raschia, Guillaume; Valduriez, Patrick; Mouaddib, Noureddine

    As the amount of stored data increases, data localization techniques become no longer sufficient in P2P systems. A practical approach is to rely on compact database summaries rather than raw database records, whose access is costly in large P2P systems. In this chapter, we describe a solution for managing linguistic data summaries in advanced P2P applications which are dealing with semantically rich data. The produced summaries are synthetic, multidimensional views over relational tables. The novelty of this proposal relies on the double summary exploitation in distributed P2P systems. First, as semantic indexes, they support locating relevant nodes based on their data descriptions. Second, due to their intelligibility, these summaries can be directly queried and thus approximately answer a query without the need for exploring original data. The proposed solution consists first in defining a summary model for hierarchical P2P systems. Second, appropriate algorithms for summary creation and maintenance are presented. A query processing mechanism, which relies on summary querying, is then proposed to demonstrate the benefits that might be obtained from summary exploitation.

  12. Behavioural Correlation for Detecting P2P Bots

    CERN Document Server

    Al-Hammadi, Yousof

    2010-01-01

    In the past few years, IRC bots, malicious programs which are remotely controlled by the attacker through IRC servers, have become a major threat to the Internet and users. These bots can be used in different malicious ways such as issuing distributed denial of services attacks to shutdown other networks and services, keystrokes logging, spamming, traffic sniffing cause serious disruption on networks and users. New bots use peer to peer (P2P) protocols start to appear as the upcoming threat to Internet security due to the fact that P2P bots do not have a centralized point to shutdown or traceback, thus making the detection of P2P bots is a real challenge. In response to these threats, we present an algorithm to detect an individual P2P bot running on a system by correlating its activities. Our evaluation shows that correlating different activities generated by P2P bots within a specified time period can detect these kind of bots.

  13. A Framework For Concept Drifting P2P Traffic Identification

    Directory of Open Access Journals (Sweden)

    Guanghui Yan

    2013-08-01

    Full Text Available Identification of network traffic using port-based or payload-based analysis is becoming increasing difficult with many Peer-to-Peer (P2P application using dynamic ports, masquerading techniques, and encryption to avoid detection. To overcome this problem, several machine learning technique were proposed to classify P2P traffics. But in the real P2P network environment, new communities of peers often attend and old communities of peers often leave. It requires the identification methods to be capable of coping with concept drift, and updating the model incrementally. In this paper, we present a concept-adapting algorithm CluMC which is based on streaming data mining techniques to identify P2P applications in Internet traffic. The CluMC use micro-cluster structures which contain potential micro-cluster structures and outlier micro-cluster structures to classify the P2P traffic and discover the concept drift with limited memory. Our performance study over a number of real data sets that we captured at a main gateway router demonstrates the effectiveness and efficiency of our method.

  14. Electronic structure of Fe2P(10 1 bar 0) studied by soft X-ray photoelectron spectroscopy and X-ray absorption spectroscopy

    Science.gov (United States)

    Sugizaki, Y.; Motoyama, H.; Edamoto, K.; Ozawa, K.

    2017-10-01

    The electronic structure of Fe2P(10 1 bar 0) has been investigated by photoelectron spectroscopy (PES) and X-ray absorption spectroscopy (XAS). The surface prepared by cycles of Ar+ ion sputtering and annealing at 500-800 °C showed a c(2 × 2) low-energy electron diffraction (LEED) pattern. An Fe 3d-P 3p hybrid band (main band) and a satellite were observed at 0-4 eV and 5-8 eV, respectively, in PES spectra of c(2 × 2) Fe2P(10 1 bar 0). The main band showed a clear cut-off at the Fermi edge, indicating the metallic nature of Fe2P. The satellite intensity showed a resonant maximum around the Fe 3p threshold, suggesting that the satellite is caused through a shake-up process. Three types of surface-shifted components were found in P 2p PES spectra. All the P 2p peaks have symmetric line shapes, while the Fe 2p PES and Fe L-edge XAS spectra have asymmetric line shapes, suggesting that the electronic states around the Fermi level are mostly composed of Fe 3d components. These results suggest that the stabilization of the electronic structure at metal sites through the bonding with P atoms is ineffective on Fe2P(10 1 bar 0), as in the case of Fe2P(0001).

  15. Measurement of the spin-forbidden decay rate (3s3d)1D2¿(3s3p)3 P2,1 in 24Mg

    DEFF Research Database (Denmark)

    Therkildsen, K. T.; Jensen, Brian Bak; Ryder, C. P.;

    2009-01-01

    We have measured the spin-forbidden decay rate from (3s3d)D12¿(3s3p)P32,1 in M24g atoms trapped in a magneto-optical trap. The total decay rate, summing up both exit channels (3s3p)P31 and (3s3p)P32 , yields 196±10s-1 in excellent agreement with resent relativistic many-body calculations of Porsev...

  16. Market Design for a P2P Backup System

    Science.gov (United States)

    Seuken, Sven; Charles, Denis; Chickering, Max; Puri, Sidd

    Peer-to-peer (P2P) backup systems are an attractive alternative to server-based systems because the immense costs of large data centers can be saved by using idle resources on millions of private computers instead. This paper presents the design and theoretical analysis of a market for a P2P backup system. While our long-term goal is an open resource exchange market using real money, here we consider a system where monetary transfers are prohibited. A user who wants to backup his data must in return supply some of his resources (storage space, upload and download bandwidth) to the system.We propose a hybrid P2P architecture where all backup data is transferred directly between peers, but a dedicated server coordinates all operations and maintains meta-data. We achieve high reliability guarantees while keeping our data replication factor low by adopting sophisticated erasure coding technology (cf., [2]).

  17. PROSE: Proactive, Selective CDN Participation for P2P Streaming

    Institute of Scientific and Technical Information of China (English)

    Zhi-Hui Lv; Li-Jiang Chen; Jie Wu; Da Deng; Si-Jia Huang; Yi Huang

    2013-01-01

    Many production peer-to-peer (P2P) streaming systems use content delivery networks (CDN) to protect the user's quality of experiences.Thus,how to efficiently utilize the capacity of CDN (e.g.,which peers receive services from the CDN nodes) is a problem of practical significance.Existing solutions adopt a passive,on-demand approach,which is inefficient iu utilizing CDN resources.In this paper,we propose PROSE,a simple,novel scheme to achieve proactive,selective CDN participation for P2P streaming.PROSE introduces novel concepts such as choke point expansion nodes/super nodes and leads to efficient,light-weighted,and distributed algorithms to identify and serve these nodes using CDN.Our experimental results show that PROSE achieves at least 10%~25% performance improvement and 2~4 times overhead reduction compared with existing general CDN-P2P-hybrid schemes.

  18. Model of Controlling the Hubs in P2P Networks

    Directory of Open Access Journals (Sweden)

    Yuhua Liu

    2009-06-01

    Full Text Available Research into the hubs in Peer-to-Peer (P2P networks, and present a new method to avoid generating the hubs in the networks by controlling the logical topology structure of P2P networks. We firstly introduce the controlling ideas about hierarchizing the hubs. Then, we disclose and interpret the controlling model, and give out the concrete method to carry it out. Finally, we validate our controlling model via simulations and the simulation results demonstrate that our work is effective to control the hubs in P2P networks. Thus, this model can improve the network competence to defend against coordinated attacks, promote the network robustness, and ensure the network would develop continually and healthily.

  19. On Using Seeders for P2P Live Streaming

    CERN Document Server

    Mathieu, Fabien

    2011-01-01

    Seeders (peers that do not request anything but contribute to the system) are a powerful concept in peer-to-peer (P2P). They allow to leverage the capacities of a P2P system. While seeding is a natural idea for filesharing or video-on-demand applications, it seems somehow counter-intuitive in the context of live streaming. This paper aims at describing the feasibility and performance of P2P live seeding. After a formal definition of "live seeding" and efficiency, we consider the theoretical performance of systems where the overhead is neglected. We then propose a linear overhead model and extend the results for this model, for a single seeder and for a set of seeders as well (it is not always possible to perfectly aggregate individual efficiencies in a given system).

  20. Compromising Tor Anonymity Exploiting P2P Information Leakage

    CERN Document Server

    Manils, Pere; Blond, Stevens Le; Kaafar, Mohamed Ali; Castelluccia, Claude; Legout, Arnaud; Dabbous, Walid

    2010-01-01

    Privacy of users in P2P networks goes far beyond their current usage and is a fundamental requirement to the adoption of P2P protocols for legal usage. In a climate of cold war between these users and anti-piracy groups, more and more users are moving to anonymizing networks in an attempt to hide their identity. However, when not designed to protect users information, a P2P protocol would leak information that may compromise the identity of its users. In this paper, we first present three attacks targeting BitTorrent users on top of Tor that reveal their real IP addresses. In a second step, we analyze the Tor usage by BitTorrent users and compare it to its usage outside of Tor. Finally, we depict the risks induced by this de-anonymization and show that users' privacy violation goes beyond BitTorrent traffic and contaminates other protocols such as HTTP.

  1. Mobile P2P Web Services Using SIP

    Directory of Open Access Journals (Sweden)

    Guido Gehlen

    2007-01-01

    Full Text Available Telecommunication networks and the Internet are growing together. Peer-to-Peer (P2P services which are originally offered by network providers, like telephony and messaging, are provided through VoIP and Instant Messaging (IM by Internet service providers, too. The IP Multimedia Subsystem (IMS is the answer of the telecommunication industry to this trend and aims at providing Internet P2P and multimedia services controlled by the network operators. The IMS provides mobility and session management as well as message routing, security, and billing.

  2. On the {P2, P3}-Factor of Cubic Graphs

    Institute of Scientific and Technical Information of China (English)

    GOU Kui-xiang; SUN Liang

    2005-01-01

    Let G = ( V, E) be a finite simple graph and Pn denote the path of order n. A spanning subgraph F is called a {P2, P3}-factor of G if each component of F is isomorphic to P2 or P3. With the path-covering method, it is proved that any connected cubic graph with at least 5 vertices has a { P2, P3 }-factor F such that | P3 (F) |≥|P2 (F) |, where P2 (F) and P3 (F) denote the set of components of P2 and P3 in F,respectively.

  3. Phosphatidic acid and phosphoinositides facilitate liposome association of Yas3p and potentiate derepression of ARE1 (alkane-responsive element one)-mediated transcription control.

    Science.gov (United States)

    Kobayashi, Satoshi; Hirakawa, Kiyoshi; Horiuchi, Hiroyuki; Fukuda, Ryouichi; Ohta, Akinori

    2013-12-01

    In the n-alkane assimilating yeast Yarrowia lipolytica, the expression of ALK1, encoding a cytochrome P450 that catalyzes terminal mono-oxygenation of n-alkanes, is induced by n-alkanes. The transcription of ALK1 is regulated by a heterocomplex that comprises the basic helix-loop-helix transcription activators, Yas1p and Yas2p, and binds to alkane-responsive element 1 (ARE1) in the ALK1 promoter. An Opi1 family transcription repressor, Yas3p, represses transcription by binding to Yas2p. Yas3p localizes in the nucleus when Y. lipolytica is grown on glucose but localizes to the endoplasmic reticulum (ER) upon the addition of n-alkanes. In this study, we showed that recombinant Yas3p binds to the acidic phospholipids, phosphatidic acid (PA) and phosphoinositides (PIPs), in vitro. The ARE1-mediated transcription was enhanced in vivo in mutants defective in an ortholog of the Saccharomyces cerevisiae gene PAH1, encoding PA phosphatase, and in an ortholog of SAC1, encoding PIP phosphatase in the ER. Truncation mutation analyses for Yas3p revealed two regions that bound to PA and PIPs. These results suggest that the interaction with acidic phospholipids is important for the n-alkane-induced association of Yas3p with the ER membrane.

  4. Association of Serum MiR-142-3p and MiR-101-3p Levels with Acute Cellular Rejection after Heart Transplantation

    Science.gov (United States)

    Sukma Dewi, Ihdina; Hollander, Zsuzsanna; Lam, Karen K.; McManus, Janet-Wilson; Tebbutt, Scott J.; Ng, Raymond T.; Keown, Paul A.; McMaster, Robert W.; McManus, Bruce M.; Gidlöf, Olof; Öhman, Jenny

    2017-01-01

    Background Identifying non-invasive and reliable blood-derived biomarkers for early detection of acute cellular rejection in heart transplant recipients is of great importance in clinical practice. MicroRNAs are small molecules found to be stable in serum and their expression patterns reflect both physiological and underlying pathological conditions in human. Methods We compared a group of heart transplant recipients with histologically-verified acute cellular rejection (ACR, n = 26) with a control group of heart transplant recipients without allograft rejection (NR, n = 37) by assessing the levels of a select set of microRNAs in serum specimens. Results The levels of seven microRNAs, miR-142-3p, miR-101-3p, miR-424-5p, miR-27a-3p, miR-144-3p, miR-339-3p and miR-326 were significantly higher in ACR group compared to the control group and could discriminate between patients with and without allograft rejection. MiR-142-3p and miR-101-3p had the best diagnostic test performance among the microRNAs tested. Serum levels of miR-142-3p and miR-101-3p were independent of calcineurin inhibitor levels, as measured by tacrolimus and cyclosporin; kidney function, as measured by creatinine level, and general inflammation state, as measured by CRP level. Conclusion This study demonstrated two microRNAs, miR-142-3p and miR-101-3p, that could be relevant as non-invasive diagnostic tools for identifying heart transplant patients with acute cellular rejection. PMID:28125729

  5. Silane photoabsorption spectra near the Si 2p thresholds: the geometry of Si 2p excited SiH4

    Institute of Scientific and Technical Information of China (English)

    张卫华; 许如清; 李家明

    2003-01-01

    Based on the multiple-scattering self-consistent-field method, we have studied the photoabsorption spectra near the Si 2p thresholds of silane. According to our calculations, the clear assignments of the inner-shell photoabsorption spectra are provided. In comparison with the high-resolution experimental spectra, the geometric structure of the Si 2p-excited SiH4** is recommended to be of a C2v symmetry. More specifically, the Si 2p-excited Si4** have two bond lengths of 2.50 a.u. and another two bond lengths of 2.77 a.u., and the corresponding two bond angles are 104.0° and 112.5° respectively.

  6. Measurement and Analysis of P2P IPTV Program Resource

    Directory of Open Access Journals (Sweden)

    Wenxian Wang

    2014-01-01

    Full Text Available With the rapid development of P2P technology, P2P IPTV applications have received more and more attention. And program resource distribution is very important to P2P IPTV applications. In order to collect IPTV program resources, a distributed multi-protocol crawler is proposed. And the crawler has collected more than 13 million pieces of information of IPTV programs from 2009 to 2012. In addition, the distribution of IPTV programs is independent and incompact, resulting in chaos of program names, which obstructs searching and organizing programs. Thus, we focus on characteristic analysis of program resources, including the distributions of length of program names, the entropy of the character types, and hierarchy depth of programs. These analyses reveal the disorderly naming conventions of P2P IPTV programs. The analysis results can help to purify and extract useful information from chaotic names for better retrieval and accelerate automatic sorting of program and establishment of IPTV repository. In order to represent popularity of programs and to predict user behavior and popularity of hot programs over a period, we also put forward an analytical model of hot programs.

  7. P2P Domain Classification using Decision Tree

    CERN Document Server

    Ismail, Anis

    2011-01-01

    In Peer-to-Peer context, a challenging problem is how to find the appropriate peer to deal with a given query without overly consuming bandwidth? Different methods proposed routing strategies of queries taking into account the P2P network at hand. This paper considers an unstructured P2P system based on an organization of peers around Super-Peers that are connected to Super-Super- Peer according to their semantic domains; By analyzing the queries log file, a predictive model that avoids flooding queries in the P2P network is constructed after predicting the appropriate Super-Peer, and hence the peer to answer the query. A challenging problem in a schema-based Peer-to-Peer (P2P) system is how to locate peers that are relevant to a given query. In this paper, architecture, based on (Super-)Peers is proposed, focusing on query routing. The approach to be implemented, groups together (Super-)Peers that have similar interests for an efficient query routing method. In such groups, called Super-Super-Peers (SSP), Su...

  8. Comparing Pedophile Activity in Different P2P Systems

    Directory of Open Access Journals (Sweden)

    Raphaël Fournier

    2014-07-01

    Full Text Available Peer-to-peer (P2P systems are widely used to exchange content over the Internet. Knowledge of pedophile activity in such networks remains limited, despite having important social consequences. Moreover, though there are different P2P systems in use, previous academic works on this topic focused on one system at a time and their results are not directly comparable. We design a methodology for comparing KAD and eDonkey, two P2P systems among the most prominent ones and with different anonymity levels. We monitor two eDonkey servers and the KAD network during several days and record hundreds of thousands of keyword-based queries. We detect pedophile-related queries with a previously validated tool and we propose, for the first time, a large-scale comparison of pedophile activity in two different P2P systems. We conclude that there are significantly fewer pedophile queries in KAD than in eDonkey (approximately 0.09% vs. 0.25%.

  9. Measurement and Analysis of P2P IPTV Program Resource

    Science.gov (United States)

    Chen, Xingshu; Wang, Haizhou; Zhang, Qi

    2014-01-01

    With the rapid development of P2P technology, P2P IPTV applications have received more and more attention. And program resource distribution is very important to P2P IPTV applications. In order to collect IPTV program resources, a distributed multi-protocol crawler is proposed. And the crawler has collected more than 13 million pieces of information of IPTV programs from 2009 to 2012. In addition, the distribution of IPTV programs is independent and incompact, resulting in chaos of program names, which obstructs searching and organizing programs. Thus, we focus on characteristic analysis of program resources, including the distributions of length of program names, the entropy of the character types, and hierarchy depth of programs. These analyses reveal the disorderly naming conventions of P2P IPTV programs. The analysis results can help to purify and extract useful information from chaotic names for better retrieval and accelerate automatic sorting of program and establishment of IPTV repository. In order to represent popularity of programs and to predict user behavior and popularity of hot programs over a period, we also put forward an analytical model of hot programs. PMID:24772008

  10. Theoretical analysis of the magnetic circular dichroism in the 2p3d and 2p4d x-ray emission of Gd

    NARCIS (Netherlands)

    Groot, F.M.F. de; Nakazawa, M.; Kotani, A.; Krisch, M.H.; Sette, F.

    1997-01-01

    The 2p3d and 2p4d x-ray emission spectral shapes have been calculated using a theoretical description of spin-polarized 2p photoemission and atomic multiplet calculations of the 2p3d and 2p4d radiative decay. Emphasis is given to the use of circular-polarized x rays for the excitation process. Good

  11. Treatment of refractory anemia with ring sideroblasts associated with marked thrombocytosis with lenalidomide in a patient testing negative for 5q deletion and JAK2 V617F and MPL W515K/L mutations

    Directory of Open Access Journals (Sweden)

    Ryan Keen

    2016-11-01

    Full Text Available Refractory anemia with ring sideroblasts associated with marked thrombocytosis (RARS-T is a hematologic malignancy that often results in transfusion dependency and a hypercoagulable state. This rare disease currently lacks formal guidelines for treatment; however, various case reports have demonstrated efficacy in the use of lenalidomide. This immunomodulatory drug has shown promise in patients with 5q deletions, with reports of achieving transfusion independence and normalization of platelet counts. Herein we present the case of a 68-year-old African American woman with RARS-T who tested negative for 5q deletion and JAK2 V617F and MPL W515K/L mutations. Her treatment with lenalidomide therapy resulted in a five-year durable complete clinical response.

  12. MEF2C haploinsufficiency caused by either microdeletion of the 5q14.3 region or mutation is responsible for severe mental retardation with stereotypic movements, epilepsy and/or cerebral malformations

    Science.gov (United States)

    Le Meur, Nathalie; Holder-Espinasse, Muriel; Jaillard, Sylvie; Goldenberg, Alice; Joriot, Sylvie; Amati-Bonneau, Patrizia; Guichet, Agnès; Barth, Magalie; Charollais, Aude; Journel, Hubert; Auvin, Stéphane; Boucher, Cécile; Kerckaert, Jean-Pierre; David, Véronique; Manouvrier-Hanu, Sylvie; Saugier-Veber, Pascale; Frébourg, Thierry; Dubourg, Christèle; Andrieux, Joris; Bonneau, Dominique

    2010-01-01

    Over the last few years, array-CGH has remarkably improved the ability to detect cryptic unbalanced rearrangements in patients presenting with syndromic mental retardation. Using whole genome oligonucleotide array-CGH, we detected 5q14.3 microdeletions ranging from 216 kb to 8.8 Mb in 5 unrelated patients showing phenotypic similarities, namely severe mental retardation with absent speech, hypotonia and stereotypic movements. Most of the patients presented also with facial dysmorphic features, epilepsy and/or cerebral malformations. The minimal common deleted region of these 5q14 microdeletions encompassed only MEF2C, known to act in brain as a neurogenesis effector which regulates excitatory synapse number. In a patient presenting a similar phenotype, we subsequently identified a MEF2C nonsense mutation. Taken together, these results strongly suggest that haploinsufficiency of MEF2C is responsible for severe mental retardation with stereotypic movements, seizures and/or cerebral malformations. PMID:19592390

  13. ICL-induced miR139-3p and miR199a-3p have opposite roles in hematopoietic cell expansion and leukemic transformation.

    Science.gov (United States)

    Alemdehy, Mir Farshid; Haanstra, Jurgen R; de Looper, Hans W J; van Strien, Paulina M H; Verhagen-Oldenampsen, Judith; Caljouw, Yvette; Sanders, Mathijs A; Hoogenboezem, Remco; de Ru, Arnoud H; Janssen, George M C; Smetsers, Stephanie E; Bierings, Marc B; van Veelen, Peter A; von Lindern, Marieke; Touw, Ivo P; Erkeland, Stefan J

    2015-06-18

    Interstrand crosslinks (ICLs) are toxic DNA lesions that cause severe genomic damage during replication, especially in Fanconi anemia pathway-deficient cells. This results in progressive bone marrow failure and predisposes to acute myeloid leukemia (AML). The molecular mechanisms responsible for these defects are largely unknown. Using Ercc1-deficient mice, we show that Trp53 is responsible for ICL-induced bone marrow failure and that loss of Trp53 is leukemogenic in this model. In addition, Ercc1-deficient myeloid progenitors gain elevated levels of miR-139-3p and miR-199a-3p with age. These microRNAs exert opposite effects on hematopoiesis. Ectopic expression of miR-139-3p strongly inhibited proliferation of myeloid progenitors, whereas inhibition of miR-139-3p activity restored defective proliferation of Ercc1-deficient progenitors. Conversely, the inhibition of miR-199a-3p functions aggravated the myeloid proliferation defect in the Ercc1-deficient model, whereas its enforced expression enhanced proliferation of progenitors. Importantly, miR-199a-3p caused AML in a pre-leukemic mouse model, supporting its role as an onco-microRNA. Target genes include HuR for miR-139-3p and Prdx6, Runx1, and Suz12 for miR-199a-3p. The latter genes have previously been implicated as tumor suppressors in de novo and secondary AML. These findings show that, in addition to TRP53-controlled mechanisms, miR-139-3p and miR-199a-3p are involved in the defective hematopoietic function of ICL-repair deficient myeloid progenitors.

  14. Experimental cross sections for two-electron capture into nitrogen autoionising states in Nsup(q+) (q=6,7) on He and H/sub 2/ collisions at 10. 5q keV

    Energy Technology Data Exchange (ETDEWEB)

    Bordenave-Montesquieu, A.; Benoit-Cattin, P.; Gleizes, A.; Marrakchi, A.I.; Dousson, S.; Hitz, D.

    1985-07-01

    Singly differential cross sections for two-electron capture into autoionising states (nl,n'l') with n=2,3,4 and n'>=n in Nsup(q+) (q=6,7) on He and H/sub 2/ collisions have been measured at 10,5q ke V collision energy and an observation angle thetasub(lab)=11.6/sup 0/. Total cross sections are estimated assuming isotropic angular distributions. (orig.).

  15. Theatrical distribution and P2P movie piracy: a survey of P2P networks in Hungary using transactional data

    NARCIS (Netherlands)

    Bodó, B.; Lakatos, Z.

    2012-01-01

    This article examines what appears to be the most important factor shaping file sharing: the failure of traditional cultural markets to efficiently supply the demand in the online environment. Its findings are based on tracking the traffic of movies on three Hungarian P2P networks. This dataset is t

  16. Theatrical distribution and P2P movie piracy: a survey of P2P networks in Hungary using transactional data

    NARCIS (Netherlands)

    Bodó, B.; Lakatos, Z.

    2012-01-01

    This article examines what appears to be the most important factor shaping file sharing: the failure of traditional cultural markets to efficiently supply the demand in the online environment. Its findings are based on tracking the traffic of movies on three Hungarian P2P networks. This dataset is t

  17. Evaluation of chromosome 5 aberrations in complex karyotypes of patients with myeloid disorders reveals their contribution to dicentric and tricentric chromosomes, resulting in the loss of critical 5q regions.

    Science.gov (United States)

    Herry, Angèle; Douet-Guilbert, Nathalie; Morel, Frédéric; Le Bris, Marie-Josée; Morice, Patrick; Abgrall, Jean François; Berthou, Christian; De Braekeleer, Marc

    2007-06-01

    Dicentric chromosomes have often been observed in complex karyotypes in previously reported studies of therapy-related myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). Fluorescence in situ hybridization (FISH) has now made the characterization of these rearrangements much easier. Dicentric and tricentric chromosomes were identified in 21 patients (9 MDS and 12 AML) among the 133 consecutive MDS/AML patients (17%) who had a structural or numerical aberration of chromosome 5 using conventional cytogenetic analysis. One third (7/21) of the patients had received alkylating drugs for a previously diagnosed cancer or chronic myeloproliferative disease. Loss of 5q material was identified in all 21 patients. One copy of the EGR1 (5q31) or the CSF1R (5q33 approximately q34) genes was lost in 20 of the 21 patients. Dicentric and tricentric chromosomes involving chromosome 5 are frequently observed in complex karyotypes among patients with de novo or therapy-related MDS/AML. They lead to deletions of various parts of the long arm of chromosome 5.

  18. Syntenic homology of human unique DNA sequences within chromossome regions 5q31, 10q22, 13q32-33 and 19q13.1 in the great apes

    Directory of Open Access Journals (Sweden)

    Vallente-Samonte Rhea U.

    2000-01-01

    Full Text Available Homologies between chromosome banding patterns and DNA sequences in the great apes and humans suggest an apparent common origin for these two lineages. The availability of DNA probes for specific regions of human chromosomes (5q31, 10q22, 13q32-33 and 19q13.1 led us to cross-hybridize these to chimpanzee (Pan troglodytes, PTR, gorilla (Gorilla gorilla, GGO and orangutan (Pongo pygmaeus, PPY chromosomes in a search for equivalent regions in the great apes. Positive hybridization signals to the chromosome 5q31-specific DNA probe were observed at HSA 5q31, PTR 4q31, GGO 4q31 and PPY 4q31, while fluorescent signals using the chromosome 10q22-specific DNA probe were noted at HSA 10q22, PTR 8q22, GGO 8q22 and PPY 7q22. The chromosome arms showing hybridization signals to the Quint-EssentialTM 13-specific DNA probe were identified as HSA 13q32-33, PTR 14q32-33, GGO 14q32-33 and PPY 14q32-33, while those presenting hybridization signals to the chromosome 19q13.1-specific DNA probe were identified as HSA 19q13.1, PTR 20q13, GGO 20q13 and PPY 20q13. All four probes presumably hybridized to homologous chromosomal locations in the apes, which suggests a homology of certain unique DNA sequences among hominoid species.

  19. MSPnet: MANAGEABLE SIP P2P MEDIA DISTRIBUTION SYSTEM

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The letter proposes a three-layer manageable media distribution network system architecture called MSPnet, which is based on Session Initiation Protocol[1] and Peer to Peer (SIP P2P)technology. MSPnet performs application-level structured DHT routing and resource location among domains and unstructured ones in domain. Except for media distribution, it can be used to support a variety of P2P applications, including video broadcasting, video on demand, VoIP, etc. MSPnet is composed of three layers, namely, the signal control layer, the management layer, and the media transportation layer. The MSPnet prototype consists of the SIP server, the management server, the media server, and the node User Agent (UA). Results from a prototype experiment in a large-scale Internet environment show that MSPnet is feasible, scalable and manageable.

  20. Fine Structure of the Upsilon 2p States.

    Science.gov (United States)

    Lovelock, Dale Michael John

    1990-01-01

    The Columbia-Stony Brook (CUSB) collaboration has measured the photon spectrum resulting from the decays Upsilon (3S)togamma + hadrons using the CUSB-II BGO calorimeter. The spectrum contains the signal from the electromagnetic transitions Upsilon (3S)togammachi _{b,J=0,1,2} (2P). From this signal the masses of the chi_{b}(2P) states were determined to be 10268.0 +/- 0.8, 10255.4 +/- 0.8, and 10233.9 +/- 1.2 MeV for the J = 2, 1, and 0 states respectively. The ratio of the mass splittings, (M_{chi _{b2}} - M_{chi_{b1}} )/(M_{chi_{b1 }} - M_ {chi_{b0}}) imply that the long range confining piece of the inter-quark potential transforms as a Lorentz scalar.

  1. Mobile P2P Trusted On-Demand Video Streaming

    CERN Document Server

    Iyer, Thava; Rizvandi, Nikzad Babaii; Varghese, Benoy; Boreli, Roksana

    2012-01-01

    We propose to demonstrate a mobile server assisted P2P system for on-demand video streaming. Our proposed solution uses a combination of 3G and ad-hoc Wi-Fi connections, to enable mobile devices to download content from a centralised server in a way that minimises the 3G bandwidth use and cost. On the customised GUI, we show the corresponding reduction in 3G bandwidth achieved by increasing the number of participating mobile devices in the combined P2P and ad-hoc Wi- Fi network, while demonstrating the good video playout quality on each of the mobiles. We also demonstrate the implemented trust mechanism which enables mobiles to only use trusted adhoc connections. The system has been implemented on Android based smartphones.

  2. Supporting Seamless Mobility for P2P Live Streaming

    Directory of Open Access Journals (Sweden)

    Eunsam Kim

    2014-01-01

    Full Text Available With advent of various mobile devices with powerful networking and computing capabilities, the users' demand to enjoy live video streaming services such as IPTV with mobile devices has been increasing rapidly. However, it is challenging to get over the degradation of service quality due to data loss caused by the handover. Although many handover schemes were proposed at protocol layers below the application layer, they inherently suffer from data loss while the network is being disconnected during the handover. We therefore propose an efficient application-layer handover scheme to support seamless mobility for P2P live streaming. By simulation experiments, we show that the P2P live streaming system with our proposed handover scheme can improve the playback continuity significantly compared to that without our scheme.

  3. Supporting seamless mobility for P2P live streaming.

    Science.gov (United States)

    Kim, Eunsam; Kim, Sangjin; Lee, Choonhwa

    2014-01-01

    With advent of various mobile devices with powerful networking and computing capabilities, the users' demand to enjoy live video streaming services such as IPTV with mobile devices has been increasing rapidly. However, it is challenging to get over the degradation of service quality due to data loss caused by the handover. Although many handover schemes were proposed at protocol layers below the application layer, they inherently suffer from data loss while the network is being disconnected during the handover. We therefore propose an efficient application-layer handover scheme to support seamless mobility for P2P live streaming. By simulation experiments, we show that the P2P live streaming system with our proposed handover scheme can improve the playback continuity significantly compared to that without our scheme.

  4. Addressing the P2P Bootstrap Problem for Small Networks

    CERN Document Server

    Wolinsky, David Isaac; Boykin, P Oscar; Figueiredo, Renato

    2010-01-01

    P2P overlays provide a framework for building distributed applications consisting of few to many resources with features including self-configuration, scalability, and resilience to node failures. Such systems have been successfully adopted in large-scale services for content delivery networks, file sharing, and data storage. In small-scale systems, they can be useful to address privacy concerns and for network applications that lack dedicated servers. The bootstrap problem, finding an existing peer in the overlay, remains a challenge to enabling these services for small-scale P2P systems. In large networks, the solution to the bootstrap problem has been the use of dedicated services, though creating and maintaining these systems requires expertise and resources, which constrain their usefulness and make them unappealing for small-scale systems. This paper surveys and summarizes requirements that allow peers potentially constrained by network connectivity to bootstrap small-scale overlays through the use of e...

  5. P2P Networks with IP Based Communication

    OpenAIRE

    Anupriya Koneru; Krishna Prasad MHM

    2012-01-01

    P2P communities can be seen as truly Distributed Computing applications in which group members communicate with one another to exchange information. The authors consider security issues in Peer to Peer Networks. For secure exchange of data between the group members the authors present a cryptography protocol and an Identity mechanism which can able to check even the Trust of the Peers based on the available reputation information. The authors are encapsulating the reputations of both the prov...

  6. Rotation Symmetric Bent Boolean Functions for n = 2p

    OpenAIRE

    Cusick, T. W.; Sanger, E. M.

    2017-01-01

    It has been conjectured that there are no homogeneous rotation symmetric bent Boolean functions of degree greater than two. In this paper we begin by proving that sums of short-cycle rotation symmetric bent Boolean functions must contain a specific degree two monomial rotation symmetric Boolean function. We then prove most cases of the conjecture in n=2p, p>2 prime, variables and extend this work to the nonhomogeneous case.

  7. Pure P2P mediation system: A mappings discovery approach

    Science.gov (United States)

    selma, El yahyaoui El idrissi; Zellou, Ahmed; Idri, Ali

    2015-02-01

    The information integration systems consist in offering a uniform interface to provide access to a set of autonomous and distributed information sources. The most important advantage of this system is that it allows users to specify what they want, rather than thinking about how to get the responses. The works realized in this area have particular leads to two major classes of integration systems: the mediation systems based on the paradigm mediator / adapter and peer to peer systems (P2P). The combination of both systems has led to a third type; is the mediation P2P systems. The P2P systems are large-scale systems, self-organized and distributed. They allow the resource management in a completely decentralized way. However, the integration of structured information sources, heterogeneous and distributed proves to be a complex problem. The objective of this work is to propose an approach to resolve conflicts and establish a mapping between the heterogeneous elements. This approach is based on clustering; the latter is to group similar Peers that share common information in the same subnet. Thus, to facilitate the heterogeneity, we introduced three additional layers of our hierarchy of peers: internal schema, external schema and Schema directory peer. We used linguistic techniques, and precisely the name correspondence technique, that is based on the similarity of names to propose a correspondence.

  8. Uniform Sampling for Directed P2P Networks

    Science.gov (United States)

    Hall, Cyrus; Carzaniga, Antonio

    Selecting a random peer with uniform probability across a peer-to-peer (P2P) network is a fundamental function for unstructured search, data replication, and monitoring algorithms. Such uniform sampling is supported by several techniques. However, current techniques suffer from sample bias and limited applicability. In this paper, we present a sampling algorithm that achieves a desired uniformity while making essentially no assumptions about the underlying P2P network. This algorithm, called doubly stochastic converge (DSC), iteratively adjusts the probabilities of crossing each link in the network during a random walk, such that the resulting transition matrix is doubly stochastic. DSC is fully decentralized and is designed to work on both directed and undirected topologies, making it suitable for virtually any P2P network. Our simulations show that DSC converges quickly on a wide variety of topologies, and that the random walks needed for sampling are short for most topologies. In simulation studies with FreePastry, we show that DSC is resilient to high levels of churn, while incurring a minimal sample bias.

  9. Interface reactions in Mg/Zn3P2 solar cells

    Science.gov (United States)

    Kazmerski, L. L.; Ireland, P. J.; Catalano, A.

    1981-05-01

    The composition and chemistry of the Mg/Zn3P2 interface was studied using surface analysis techniques. For higher carrier concentrations (p 10 to the 17th power/cu cm), a heterojunction occurs by the chemical interaction of the Mg with Zn3P2 to form Mg3P2. For lower carrier concentrations this reaction does not take place, and Mg diffuses into the Zn3P2. Diffusion coefficients of Mg in Zn3P2 are determined. Grain (crystalline) and grain boundary components of the diffusion coefficient are evaluated. These data compare very well with that reported using electron beam induced currents and spectral response measurements.

  10. Detection of O(3P(J)) atoms formed by reaction, Al+O2--> AlO+O under crossed-beam condition.

    Science.gov (United States)

    Ishida, Masayuki; Higashiyama, Tomohiko; Matsumoto, Yoshiteru; Honma, Kenji

    2005-05-22

    The vacuum ultraviolet laser-induced fluorescence technique was employed to detect the oxygen atoms formed by the reaction, Al+O(2)--> AlO+O. The measurements were carried out under the crossed-beam condition at 12.2 kJmol of collision energy. The relative populations of three spin-orbit states of O((3)P(J)) were determined to be 3.8, 1.0, and 0.2 for J=2, 1, and 0, respectively. They show nonstatistical populations, i.e., more population in O((3)P(2)) and less population in O((3)P(0)) than the statistical expectation. These populations were almost identical for two Al beam conditions where the relative concentrations of two spin-orbit states of Al, (2)P(1/2), and (2)P(3/2), are different. These results suggest that the reaction of Al with O(2) proceeds via an intermediate complex where the memory of the initial spin-orbit state is lost. Deviation from the statistical population of O((3)P(J)) implies the occurrence of the interaction among potential surfaces in the exit channel.

  11. Role of 2p-2h MEC excitations in superscaling

    Energy Technology Data Exchange (ETDEWEB)

    De Pace, A. E-mail: depace@to.infn.it; Nardi, M.; Alberico, W.M.; Donnelly, T.W.; Molinari, A

    2004-09-06

    Following recent studies of inclusive electron scattering from nuclei at high energies which focused on two-nucleon emission mediated by meson-exchange currents, in this work the superscaling behavior of such contributions is investigated. Comparisons are made with existing data below the quasielastic peak where at high momentum transfers scaling of the second kind is known to be excellent and scaling of the first kind is good, in the proximity of the peak where both 1p-1h and 2p-2h contributions come into play, and above the peak where inelasticity becomes important and one finds scaling violations of the two kinds.

  12. Random walk search in unstructured P2P

    Institute of Scientific and Technical Information of China (English)

    Jia Zhaoqing; You Jinyuan; Rao Ruonan; Li Minglu

    2006-01-01

    Unstructured P2P has power-law link distribution, and the random walk in power-law networks is analyzed. The analysis results show that the probability that a random walker walks through the high degree nodes is high in the power-law network, and the information on the high degree nodes can be easily found through random walk. Random walk spread and random walk search method (RWSS) is proposed based on the analysis result. Simulation results show that RWSS achieves high success rates at low cost and is robust to high degree node failure.

  13. Efficient Search in P2P File Sharing System

    Institute of Scientific and Technical Information of China (English)

    Xiao Bo; Jin Wei; Hou Mengshu

    2006-01-01

    A new routing algorithm of peer-to-peer file sharing system with routing indices was proposed, in which a node forwards a query to neighbors that are more likely to have answers based on its statistics. The proposed algorithm was tested by creating a P2P simulator and varying the input parameters, and was compared to the search algorithms using flooding (FLD) and random walk (RW). The result shows that with the proposed design, the queries are routed effectively, the network flows are reduced remarkably, and the peer-to-peer file sharing system gains a good expansibility.

  14. A new PC(sp(3))P ligand and its coordination chemistry with low-valent iron, cobalt and nickel complexes.

    Science.gov (United States)

    Zhu, Gengyu; Li, Xiaoyan; Xu, Guoqiang; Wang, Lin; Sun, Hongjian

    2014-06-21

    A new PC(sp(3))P ligand N,N'-bis(diphenylphosphino)dipyrromethane [PCH2P] (1) was prepared and its iron, cobalt and nickel chemistry was explored. Two pincer-type complexes [PCHP]Fe(H)(PMe3)2 (2) and [PCHP]Co(PMe3)2 (4) were synthesized in the reaction of with Fe(PMe3)4 and Co(Me)(PMe3)4. 1 reacted with Co(PMe3)4 and Ni(PMe3)4 to afford Co(0) and Ni(0) complexes [PCH2P]Co(PMe3)2 (3) and [PCH2P]Ni(PMe3)2 (5). The structures of complexes 2-5 were determined by X-ray diffraction.

  15. Atomic scattering in the diffraction limit: electron transfer in keV Li+-Na(3s, 3p) collisions

    DEFF Research Database (Denmark)

    Poel, Mike van der; Nielsen, C.V.; Rybaltover, M.;

    2002-01-01

    . This setup yields a momentum resolution of 0.12 an, an order of magnitude better angular resolution than previous measurements on this system. This enables us to clearly resolve Fraunhofer-type diffraction patterns in the angle DCS. In particular, the angular width of the ring structure is given by the ratio...... of the de Broglie wavelength lambda(dB) = 150 fm at a velocity v = 0.20 au and the effective atomic diameter for electron capture 2R = 20 au. Parallel AO and MO semiclassical coupled-channel calculations of the Na(3s, 3p) --> Li(2s, 2p) state-to-state collision amplitudes have been performed, and quantum...

  16. Supporting Personal Semantic Annotations in P2P Semantic Wikis

    Science.gov (United States)

    Torres, Diego; Skaf-Molli, Hala; Díaz, Alicia; Molli, Pascal

    In this paper, we propose to extend Peer-to-Peer Semantic Wikis with personal semantic annotations. Semantic Wikis are one of the most successful Semantic Web applications. In semantic wikis, wikis pages are annotated with semantic data to facilitate the navigation, information retrieving and ontology emerging. Semantic data represents the shared knowledge base which describes the common understanding of the community. However, in a collaborative knowledge building process the knowledge is basically created by individuals who are involved in a social process. Therefore, it is fundamental to support personal knowledge building in a differentiated way. Currently there are no available semantic wikis that support both personal and shared understandings. In order to overcome this problem, we propose a P2P collaborative knowledge building process and extend semantic wikis with personal annotations facilities to express personal understanding. In this paper, we detail the personal semantic annotation model and show its implementation in P2P semantic wikis. We also detail an evaluation study which shows that personal annotations demand less cognitive efforts than semantic data and are very useful to enrich the shared knowledge base.

  17. Energetics and Dynamics of the Reactions of O(3P) with Dimethyl Methylphosphonate and Saria

    Science.gov (United States)

    2009-09-15

    a SN2 -like transition geometry, Figure 3c, the axial O-C-H bond angle is slightly bent more for reaction 4, Figure 3d. The products of reaction 4 are...Energetics and Dynamics of the Reactions of O(3P) with Dimethyl Methylphosphonate and Sarin Patrick F. Conforti and Matthew Braunstein* Spectral...calculations were performed on the reaction systems O(3P) + sarin and O(3P) + dimethyl methylphosphonate (DMMP), a sarin simulant. Transition state

  18. Substitution, cooperative, and solvent effects on π pnicogen bonds in the FH(2)P and FH(2)As complexes.

    Science.gov (United States)

    An, Xiu-Lin; Li, Ran; Li, Qing-Zhong; Liu, Xiao-Feng; Li, Wen-Zuo; Cheng, Jian-Bo

    2012-09-01

    Ab initio calculations have been carried out to study the substitution effect on the π pnicogen bond in ZH(2)P-C(2)HM (Z = H, H(3)C, NC, F; M = H, CH(3), Li) dimer, cooperative effect of the π pnicogen bond and hydrogen bond in XH-FH(2)Y-C(2)H(4) (X = HO, NC, F; Y = P and As) trimer, and solvent effect on the π pnicogen bond in FH(2)P-C(2)H(2), FH(2)P-C(2)H(4), FH(2)As-C(2)H(2), and FH(2)As-C(2)H(4) dimers. The interaction energy of π pnicogen bond increases in magnitude from -1.51 kcal mol(-1) in H(3)P-C(2)H(2) dimer to -7.53 kcal mol(-1) in FH(2)P-C(2)HLi dimer at the MP2/aug-cc-pVTZ level. The π pnicogen bond is enhanced by 12-30 % due to the presence of hydrogen bond in the trimer. The π pnicogen bond is also enhanced in solvents. The natural bond orbital analysis and symmetry adapted perturbation theory (SAPT) were used to unveil the source of substitution, cooperative, and solvent effects.

  19. Stabilization of the triphosphallyl ligand η3-P3{P(O)H} at iridium via alkaline activation of P4.

    Science.gov (United States)

    Mirabello, Vincenzo; Caporali, Maria; Gonsalvi, Luca; Manca, Gabriele; Ienco, Andrea; Peruzzini, Maurizio

    2013-12-01

    The selective functionalization of the polyphosphorus moiety Ph2PCH2PPh2PPPP present as a tetrahapto-ligand in complex [Ir(dppm)(Ph2PCH2PPh2PPPP)](+) (1, dppm=Ph2PCH2PPh2) was obtained by reaction of 1 with water under basic conditions at room temperature. The formation of the new triphosphaallyl moiety η(3)-P3{P(O)H} was determined in solution by NMR spectroscopy, and confirmed in the solid state by a single-crystal X-ray structure of the stable product [Ir(κ(2)-dppm)(κ(1)-dppm)(η(3)-P3{P(O)H})] (2). In solution, 2 has a fluxional behavior attributable to the four P atoms belonging to the tetraphosphorus moiety in 1 and exhibits a chemical exchange process involving the two PPh2 moieties of the same bidentate ligand, as determined by 1D and 2D NMR spectroscopy experiments carried out at variable temperature. The mechanism of the reaction was investigated at the DFT level, which suggested a selective attack of an in-situ generated OH(-) anion on one of the non-coordinated phosphorus atoms of the P4 moiety. The reaction then evolves through an acid-assisted tautomerization, which leads to the final compound 2. Bonding analysis pointed out that the new unsubstituted P3-unit in the η(3)-P3{P(O)H} moiety behaves as a triphosphallyl ligand. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Circulating serum miRNA (miR-367-3p, miR-371a-3p, miR-372-3p and miR-373-3p) as biomarkers in patients with testicular germ cell cancer.

    Science.gov (United States)

    Syring, Isabella; Bartels, Joanna; Holdenrieder, Stefan; Kristiansen, Glen; Müller, Stefan C; Ellinger, Jörg

    2015-01-01

    Classic serum tumor markers (human chorionic gonadotropin, α1-fetoprotein and lactate dehydrogenase) have an important role in managing testicular germ cell tumor. Since only 60% of all patients with testicular germ cell tumor have elevations of these markers, there is a need for new biomarkers with greater sensitivity/specificity. miRNAs are deregulated in cancer and could serve as noninvasive serum biomarkers. We explored the role of serum miRNAs as a novel biomarker in patients with testicular germ cell tumor. Total RNA was isolated from serum. miRNA levels were quantified by quantitative real-time polymerase chain reaction. We assessed the miRNAs miR-302a-3p, 302b-3p, 302c-3p, 367-3p, 371a-3p, 372-3p and 373-3p in a subcohort of 30 patients with testicular germ cell tumor and 18 healthy subjects. Validation was performed in 76 patients treated with inguinal exploration due to suspicion of testicular germ cell tumor, of whom 59 had cancer and 17 had benign disease, and in 84 healthy male subjects. Serum miR-367-3p, 371a-3p, 372-3p and 373-3p levels were significantly increased in patients with testicular germ cell tumor compared to healthy individuals and patients with nonmalignant testicular disease. In particular miR-371a-3p allowed for sensitive (84.7%) and specific (99%) identification of patients with testicular germ cell tumor, thus, outperforming human chorionic gonadotropin or α1-fetoprotein testing. Furthermore, miR-367-3p was increased in nonseminoma compared to seminoma cases. Serum miRNA levels were increased in patients with advanced local stage and metastasis. In 9 patients with localized (clinical stage 1A) testicular germ cell tumor serum miR-371a-3p levels decreased postoperatively, indicating tumor specific release. miR-371a-3p allows for better identification of testicular germ cell tumor than α1-fetoprotein and human chorionic gonadotropin. It could be helpful for clinically managing testicular germ cell tumor, especially for monitoring

  1. miR-410-3p suppresses breast cancer progression by targeting Snail.

    Science.gov (United States)

    Zhang, Ya-Feng; Yu, Yue; Song, Wang-Zhao; Zhang, Rui-Ming; Jin, Shan; Bai, Jun-Wen; Kang, Hong-Bin; Wang, Xin; Cao, Xu-Chen

    2016-07-01

    miR-410-3p acts as an oncogene or tumor-suppressor gene in various types of cancer. However, its role in breast cancer remains unknown. In the present study, expression of miR-410-3p in 30 breast cancer and paired adjacent normal tissues was detected by RT-qPCR. The expression of miR-410-3p was downregulated in 76.7% of the breast cancer samples. To further validate the expression of miR-410-3p in breast cancer, we analyzed miR-410-3p expression profiling data set from The Cancer Genome Atlas (TCGA) including 683 breast cancer and 87 normal breast tissues. We observed that the expression of miR-410-3p was downregulated in breast cancer tissues. Next, we investigated the influence of miR-410-3p on cell proliferation by transiently transfecting the miR-410-3p mimic or inhibitor, as well as their corresponding controls in the MDA-MB-231 and MCF7 cell lines. miR-410-3p overexpression reduced cell growth, colony formation and the number of EdU-positive cells in the MDA-MB-231 cells. In contrast, inhibition of miR-410-3p in the MCF7 cells resulted in a higher proliferation rate as assessed by MTT assay, plate colony formation and EdU assays. Furthermore, miR-410-3p inhibited epithelial-mesenchymal transition. In addition, Snail was found to be a direct target of miR-410-3p based on a luciferase assay. Overexpression of Snail was able to rescue the effect of miR-410-3p in breast cancer cells. Moreover, miR‑410-3p was inversely expressed with Snail in breast cancer samples. Our data provide new knowledge regarding the role of miR-410-3p in breast cancer progression.

  2. Experimental and theoretical studies of rate coefficients for the reaction O(3P)+CH3OH at high temperatures.

    Science.gov (United States)

    Lu, Chih-Wei; Chou, Shen-Long; Lee, Yuan-Pern; Xu, Shucheng; Xu, Z F; Lin, M C

    2005-06-22

    Rate coefficients of the reaction O((3)P) + CH(3)OH in the temperature range of 835-1777 K were determined using a diaphragmless shock tube. O atoms were generated by photolysis of SO(2) with a KrF excimer laser at 248 nm or an ArF excimer laser at 193 nm; their concentrations were monitored via atomic resonance absorption excited by emission from a microwave-discharged mixture of O(2) and He. The rate coefficients determined for the temperature range can be represented by the Arrhenius equation, k(T) = (2.29 +/- 0.18) x 10(-10) exp[-(4210 +/- 100)T] cm(3) molecule(-1) s(-1); unless otherwise noted, all the listed errors represent one standard deviation in fitting. Combination of these and previous data at lower temperature shows a non-Arrhenius behavior described as the three-parameter equation, k(T) = (2.74 +/- 0.07) x 10(-18)T(2.25 +/- 0.13) exp[-(1500 +/- 90)T] cm(3)molecule(-1) s(-1). Theoretical calculations at the Becke-3-Lee-Yang-Parr (B3LYP)6-311 + G(3df,2p) level locate three transition states. Based on the energies computed with coupled clusters singles, doubles (triples) [CCSD(T)]/6-311 + G(3df,2p)B3LYP6-311 + G(3df,2p), the rate coefficients predicted with canonical variational transition state theory with small curvature tunneling corrections agree satisfactorily with the experimental observations. The branching ratios of two accessible reaction channels forming OH + CH(2)OH (1a) and OH + CH(3)O (1b) are predicted to vary strongly with temperature. At 300 K, reaction (1a) dominates, whereas reaction (1b) becomes more important than reaction (1a) above 1700 K.

  3. Sizeable beta-strength in 31Ar (beta 3p) decay

    DEFF Research Database (Denmark)

    T. Koldste, G.; Blank, B.; J. G. Borge, M.

    2014-01-01

    We present for the first time precise spectroscopic information on the recently discovered decay mode beta-delayed 3p-emission. The detection of the 3p events gives an increased sensitivity to the high energy part of the Gamow-Teller strength distribution from the decay of 31Ar revealing that as ...

  4. Cross sections and coherence terms for associative ionization of two differently excited Na(3p) atoms

    NARCIS (Netherlands)

    Meijer, H.A.J.; Pelgrim, T.J.C.; Heideman, H.G.M.; Morgenstern, R.; Andersen, N.

    1989-01-01

    The associative ionization process in thermal Na(3p)-Na(3p) encounters has been studied in a series of crossed-beam experiments where the light polarization of the two laser beams preparing the excited atoms before collision was varied independently. It is shown how in this way maximum possible info

  5. Terminal 3p deletions in two families--correlation between molecular karyotype and phenotype.

    NARCIS (Netherlands)

    Pohjola, P.; Leeuw, N. de; Penttinen, M.; Kaariainen, H.

    2010-01-01

    The 3p deletion syndrome is a rare disorder caused by deletions of different sizes in the 3p25-pter region. It is characterized by growth retardation, developmental delay, mental retardation, dysmorphism, microcephaly, and ptosis. The phenotype of individuals with deletions varies from normal to sev

  6. Normal peroxisome development from vesicles induced by truncated Hansenula polymorpha Pex3p

    NARCIS (Netherlands)

    Faber, KN; Haan, GJ; Baerends, RJS; Kram, AM; Veenhuis, M

    2002-01-01

    We show that the synthesis of the N-terminal 50 amino acids of Pex3p (Pex3p(1-50)) in Hansenula polymorpha pex3 cells is associated with the formation of vesicular membrane structures. Biochemical and ultrastructural findings suggest that the nuclear membrane is the donor membrane compartment of

  7. MEF2C haploinsufficiency caused by either microdeletion of the 5q14.3 region or mutation is responsible for severe mental retardation with stereotypic movements, epilepsy and/or cerebral malformations.

    Science.gov (United States)

    Le Meur, N; Holder-Espinasse, M; Jaillard, S; Goldenberg, A; Joriot, S; Amati-Bonneau, P; Guichet, A; Barth, M; Charollais, A; Journel, H; Auvin, S; Boucher, C; Kerckaert, J-P; David, V; Manouvrier-Hanu, S; Saugier-Veber, P; Frébourg, T; Dubourg, C; Andrieux, J; Bonneau, D

    2010-01-01

    Over the last few years, array-comparative genomic hybridisation (CGH) has considerably improved our ability to detect cryptic unbalanced rearrangements in patients with syndromic mental retardation. Molecular karyotyping of six patients with syndromic mental retardation was carried out using whole-genome oligonucleotide array-CGH. 5q14.3 microdeletions ranging from 216 kb to 8.8 Mb were detected in five unrelated patients with the following phenotypic similarities: severe mental retardation with absent speech, hypotonia and stereotypic movements. Facial dysmorphic features, epilepsy and/or cerebral malformations were also present in most of these patients. The minimal common deleted region of these 5q14 microdeletions encompassed only MEF2C, the gene for a protein known to act in brain as a neurogenesis effector, which regulates excitatory synapse number. In a patient with a similar phenotype, an MEF2C nonsense mutation was subsequently identified. Taken together, these results strongly suggest that haploinsufficiency of MEF2C is responsible for severe mental retardation with stereotypic movements, seizures and/or cerebral malformations.

  8. An Effective Calculation of Reputation in P2P Networks

    Directory of Open Access Journals (Sweden)

    RVVSV Prasad

    2009-07-01

    Full Text Available With the advent of sophisticated networking technologies and the related applications, more and more computers are getting hooked to the Internet. This is mainly for utilizing several services ranging from information sharing to electronic transactions. P2P networks which allow decentralized systems, have posed problems related to trust when transactions have to be carried out. Current literature proposes several solutions for trust management and reputation computation. The solutions base their assessment of reputations on the number of successful transactions or on the similarity of the feedbacks. There are some concerns in the feedback ratings if we are not considering the issues like number of transactions, frequency of transactions with the same peer and different peers, age of transaction, how frequently a given peer attends a common vendor, and the number of common vendors between the pairs. This paper puts forward a reputation computation system addressing these concerns. It implicitly allows detection of malicious peers. It also incorporates a corrective mechanism, if the feedbacks are from more number of malicious peers. The implementations and the results that support our claims are also presented.

  9. Survey on Distributed Data Mining in P2P Networks

    CERN Document Server

    T, Rekha Sunny

    2012-01-01

    The exponential increase of availability of digital data and the necessity to process it in business and scientific fields has literally forced upon us the need to analyze and mine useful knowledge from it. Traditionally data mining has used a data warehousing model of gathering all data into a central site, and then running an algorithm upon that data. Such a centralized approach is fundamentally inappropriate due to many reasons like huge amount of data, infeasibility to centralize data stored at multiple sites, bandwidth limitation and privacy concerns. To solve these problems, Distributed Data Mining (DDM) has emerged as a hot research area. Careful attention in the usage of distributed resources of data, computing, communication, and human factors in a near optimal fashion are paid by distributed data mining. DDM is gaining attention in peer-to-peer (P2P) systems which are emerging as a choice of solution for applications such as file sharing, collaborative movie and song scoring, electronic commerce, an...

  10. Improved Degree Search Algorithms in Unstructured P2P Networks

    Directory of Open Access Journals (Sweden)

    Guole Liu

    2012-01-01

    Full Text Available Searching and retrieving the demanded correct information is one important problem in networks; especially, designing an efficient search algorithm is a key challenge in unstructured peer-to-peer (P2P networks. Breadth-first search (BFS and depth-first search (DFS are the current two typical search methods. BFS-based algorithms show the perfect performance in the aspect of search success rate of network resources, while bringing the huge search messages. On the contrary, DFS-based algorithms reduce the search message quantity and also cause the dropping of search success ratio. To address the problem that only one of performances is excellent, we propose two memory function degree search algorithms: memory function maximum degree algorithm (MD and memory function preference degree algorithm (PD. We study their performance including the search success rate and the search message quantity in different networks, which are scale-free networks, random graph networks, and small-world networks. Simulations show that the two performances are both excellent at the same time, and the performances are improved at least 10 times.

  11. The temperature dependence of the cross section for the energy pooling process Na(3P)+Na(3P) to Na(4D)+Na(3S)

    Science.gov (United States)

    Horvatic, V.; Movre, M.; Vadla, C.

    1999-10-01

    We report the measurements of the temperature dependence of the cross section σ4D for the energy pooling process Na(3P)+Na(3P) to Na(4D)+Na(3S). The latest two, as yet undisputed, results for σ4D obtained by different authors at T = 597 K and T = 483 K suggest that this cross section decreases with increasing T, which contradicts the theory and other experiments on similar processes. To resolve this controversy and to examine the temperature trend of the cross section, we have measured the σ4D in the temperature range 567-705 K, covering the high-temperature region that has not yet been investigated experimentally. To determine σ4D we have excited sodium atoms in the quasistatic wing of the D1 line using a cw dye laser and measured the fluorescence intensity for the 4D to 3P3/2 transition, relative to the intensity of the optically thin quasistatic wing of the D2 line. The spatial distribution of the number density of the sodium atoms in the 3P3/2 state and the sodium ground-state number density were measured too. The method used for the determination of the cross section is advantageous since it entirely circumvents the need to account for the radiation trapping of 3P level radiation, which was substantial under experimental conditions of the ground-state densities being 1014-1016 cm-3. The measurements of the cross section σ4D in the investigated temperature range have shown that it increases as ~exp(-Δ E/kT). From the experiment we obtained Δ E = (608±95) cm-1, which is in excellent agreement with the energy defect (613 cm-1) for the considered process, and in fair agreement with the values which follow from recent theoretical calculations.

  12. The direct measurement of the 3 3P0-3 3P1 fine-structure interval and the gJ-factor of atomic silicon by laser magnetic resonance

    Science.gov (United States)

    Evenson, K. M.; Beltran-Lopez, V.; Ley-Koo, E.; Inguscio, M.

    1984-01-01

    The J - 1 fine structure interval and the g-factor of the 3P1 state have been determined with high precision in the present laser magnetic resonance measurements of the ground 3p2 3P multiplet of atomic Si. Delta-E(3P1-3P0) = 2,311,755.6(7) MHz, and gJ(3P1) = 1.500830(70). Single-configuration calculations of gJ for 3P1 and 3P2 yield a value for the latter which, at 1.501095, is noted to differ by an unexpectedly large margin from the experimental value.

  13. Peer behavior based proactive P2P worm detection%基于节点行为的主动 P2P 蠕虫检测

    Institute of Scientific and Technical Information of China (English)

    朱晖; 李伟华; 史豪斌

    2013-01-01

    Proactive P2P worm propagation is a serious security threat to P2P network and Internet. By researching the peer be-havior of propagating proactive P2P worm, this paper puts forward PBD(Peer Behavior based Detection)to detect proactive P2P worm. On this basis, it designs and implements a PPWDS(Proactive P2P Worm Detection System). This system adopts CUSUM algorithm to carry out real time monitoring to the outbound short link of P2P peers. Experiments show that PBD is an effective method of proactive P2P worm detect.%  主动 P2P 蠕虫的传播会对 P2P 网络以及互联网的安全造成严重威胁。通过研究主动 P2P 蠕虫传播时节点行为,提出一种基于节点行为的主动 P2P 蠕虫检测方法 PBD(Peer Behavior based Detection)。在此基础上设计和实现了一个主动 P2P 蠕虫检测系统 PPWDS(Proactive P2P Worm Detection System),该系统采用 CUSUM 算法对 P2P 节点出站短连接进行实时监控。实验表明,PBD 是检测主动 P2P 蠕虫的一种有效方法。

  14. Expression of pRb2/p130 Gene in Human Endometrial Carcinoma Cells and the Significance%子宫内膜癌中pRb2/p130的表达及意义

    Institute of Scientific and Technical Information of China (English)

    支霜; 傅芬

    2009-01-01

    Objective To investigate the expression of pRb2/p130 gene and its relationship with the patholgical clinical features of human endometrial carcinoma.Methods The levels of pRb2/P130 protein were examined with immunohistochemical SP method in 28 smples of normal endometrial tissue,20 samples of atypical endometrial hyperplasia and 30 samples of endometrial adenocarcinomas.Results The positive rate of pRb2/p130 protein in the normal endometrium,atypical endometrial hyperplasia cells and endometrial adenocarcinoma cells was respectively 82.1%,55.0% and 23.3%.pRb2/p130 protein in endometrial atypical hyperplasia or endometrial adenocarcinoma was significantly lower than that in the normal tissue (P0.05).Conclusion Descent or deletion of the expression of pRb2/p130 gene is of a frequent incidence in human endometrial carcinoma.The positive rate of pRb2/p130 expression is reversly proportionate to malignant degree of endometrial carcinoma,which indicats that pRb2/p130 plays an important role in the development and pathogenesis of endometrial carcinoma.%目的 探讨子宫内膜组织中pRb2/p130的表达及其与子宫内膜癌临床病理特征的关系.方法 应用免疫组化SP法检测pRb2/p130在28例正常增生期子宫内膜、20例不典型增生子宫内膜、30例子宫内膜腺癌组织中的表达.结果 pRb2/p130阳性表达率在正常增生期子宫内膜、不典型增生子宫内膜、子宫内膜腺癌组织中依次递减(分别为82.1%、55.0%、23.3%),且子宫内膜腺癌和不典型性增生中明显低于正常增生期子宫内膜,差异均有统计学意义(P0.05).结论 在子宫内膜癌中pRb2/p130的表达下降或缺失是一个频发事情,其恶性度愈高阳性率愈低,提示pRb2/p130与子宫内膜癌的发生、发展密切相关,并在其发病机制中起着重要的作用.

  15. Orientation of Zn3P2 films via phosphidation of Zn precursors

    Science.gov (United States)

    Katsube, Ryoji; Nose, Yoshitaro

    2017-02-01

    Orientation of solar absorber is an important factor to achieve high efficiency of thin film solar cells. In the case of Zn3P2 which is a promising absorber of low-cost and high-efficiency solar cells, (110)/(001) orientation was only reported in previous studies. We have successfully prepared (101)-oriented Zn3P2 films by phosphidation of (0001)-oriented Zn films at 350 °C. The phosphidation mechanism of Zn is discussed through STEM observations on the partially-reacted sample and the consideration of the relationship between the crystal structures of Zn and Zn3P2 . We revealed that (0001)-oriented Zn led to nucleation of (101)-oriented Zn3P2 due to the similarity in atomic arrangement between Zn and Zn3P2 . The electrical resistivity of the (101)-oriented Zn3P2 film was lower than those of (110)/(001)-oriented films, which is an advantage of the phosphidation technique to the growth processes in previous works. The results in this study demonstrated that well-conductive Zn3P2 films could be obtained by controlling orientations of crystal grains, and provide a guiding principle for microstructure control in absorber materials.

  16. Electronic and Rovibrational Quantum Chemical Analysis of C$_3$P$^-$: The Next Interstellar Anion?

    CERN Document Server

    Fortenberry, Ryan C

    2015-01-01

    C$_3$P$^-$ is analogous to the known interstellar anion C$_3$N$^-$ with phosphorus replacing the nitrogen in a simple step down the periodic table. In this work, it is shown that C$_3$P$^-$ is likely to possess a dipole-bound excited state. It has been hypothesized and observationally supported that dipole-bound excited states are an avenue through which anions could be formed in the interstellar medium. Additionally, C$_3$P$^-$ has a valence excited state that may lead to further stabilization of this molecule, and C$_3$P$^-$ has a larger dipole moment than neutral C$_3$P ($\\sim 6$ D vs. $\\sim 4$ D). As such, C$_3$P$^-$ is probably a more detectable astromolecule than even its corresponding neutral radical. Highly-accurate quantum chemical quartic force fields are also applied to C$_3$P$^-$ and its singly $^{13}$C substituted isotopologues in order to provide structures, vibrational frequencies, and spectroscopic constants that may aid in its detection.

  17. Evolution of a system sensitive to stochastic noise: P3.p cell fate in Caenorhabditis.

    Science.gov (United States)

    Pénigault, Jean-Baptiste; Félix, Marie-Anne

    2011-09-15

    The C. elegans cell lineage is overall invariant. One rare instance of variability concerns P3.p, the most anterior vulva precursor cell, which may either fuse with the epidermis without dividing, or remain competent to form vulval tissue and divide. Here we examine the evolutionary properties of this stochastic variation in P3.p fate. In the Caenorhabditis genus, high P3.p competence is ancestral and reduction in P3.p competence and division frequency occurred in C. sp. 14 and in a clade of nine species. Within this clade, the frequency of P3.p division further varies within and among species, being intermediate in C. elegans and low in C. briggsae. P3.p fate frequency is sensitive to random mutation accumulation, suggesting that this trait may evolve rapidly because of its sensitivity to mutational impact. P3.p fate depends on LIN-39/Hox5 expression and we find that the peak of LIN-39/Hox5 protein level is displaced posteriorly in C. briggsae compared to C. elegans. However, P3.p fate specification is most sensitive to the dose of EGL-20 and CWN-1, two Wnts that are secreted in a long-range gradient from the posterior end of C. elegans larvae (accompanying article). A half-dose of either of these Wnts is sufficient to affect division frequency in C. elegans N2 to levels similar to those in C. briggsae. Symmetrically, we show that an increase in Wnt dose rescues anterior competence in C. briggsae. We propose that evolutionary variation in the concentration or interpretation of the long-range Wnt gradient may be involved in the rapid evolution of P3.p fate in Caenorhabditis.

  18. Progress on the Multiphysics Capabilities of the Parallel Electromagnetic ACE3P Simulation Suite

    Energy Technology Data Exchange (ETDEWEB)

    Kononenko, Oleksiy [SLAC National Accelerator Lab., Menlo Park, CA (United States)

    2015-03-26

    ACE3P is a 3D parallel simulation suite that is being developed at SLAC National Accelerator Laboratory. Effectively utilizing supercomputer resources, ACE3P has become a key tool for the coupled electromagnetic, thermal and mechanical research and design of particle accelerators. Based on the existing finite-element infrastructure, a massively parallel eigensolver is developed for modal analysis of mechanical structures. It complements a set of the multiphysics tools in ACE3P and, in particular, can be used for the comprehensive study of microphonics in accelerating cavities ensuring the operational reliability of a particle accelerator.

  19. Elevation of circulating miR-210-3p in high-altitude hypoxic environment

    Directory of Open Access Journals (Sweden)

    Yan eYan

    2016-03-01

    Full Text Available Background: The induction of miR-210-3p, a master hypoxamir, is a consistent feature of the hypoxic response in both normal and malignant cells. However, whether miR-210-3p acts as a circulating factor in response to a hypoxic environment remains unknown. The current study aimed to examine the effect of a high-altitude hypoxic environment on circulating miR-210-3p.Methods: We examined and compared the levels of miR-210-3p using TaqMan-based qRT-PCR in both peripheral blood cells and plasma from 84 ethnic Chinese Tibetans residing at 3560 m, 46 newly arrived migrant Han Chinese (Tibet Han and 82 Han Chinese residing at 8.9 m (Nanjing Han. Furthermore, we analyzed the correlations of miR-210-3p with hematological indices. Results: The relative concentrations of miR-210-3p to internal reference U6 in blood cells were significantly higher in the Tibet Han group (1.01±0.11, P<0.001 and in the Tibetan group (1.17±0.09, P<0.001 than in the Nanjing Han group (0.51±0.04. The absolute concentrations of plasma miR-210-3p were also markedly elevated in the Tibet Han group (503.54±42.95 fmol/L, P=0.004 and in the Tibetan group (557.78±39.84 fmol/L, P<0.001 compared to the Nanjing Han group (358.39±16.16 fmol/L. However, in both blood cells and plasma, miR-210-3p levels were not significantly different between the Tibet Han group and the Tibetan group (P=0.280, P=0.620, respectively. Plasma miR-210-3p concentrations were positively correlated with miR-210-3p levels in blood cells (r=0.192, P=0.005. Furthermore, miR-210-3p levels in both blood cells and plasma showed strong positive correlations with red blood cell counts and hemoglobin and hematocrit values. Conclusion: These data demonstrated, for the first time, that miR-210-3p might act as a circulating factor in response to hypoxic environments and could be associated with human adaptation to life at high altitudes.

  20. Sizeable beta-strength in {sup 31}Ar (β3p) decay

    Energy Technology Data Exchange (ETDEWEB)

    Koldste, G.T. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Blank, B. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Borge, M.J.G.; Briz, J.A.; Carmona-Gallardo, M. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Fraile, L.M. [Grupo de Física Nuclear, Universidad Complutense, CEI Moncloa, E-28040 Madrid (Spain); Fynbo, H.O.U. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Giovinazzo, J. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Johansen, J.G. [Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C (Denmark); Jokinen, A. [Department of Physics, University of Jyväskylä, FIN-40351 Jyväskylä (Finland); Jonson, B. [Fundamental Fysik, Chalmers Tekniska Högskola, S-41296 Göteborg (Sweden); Kurturkian-Nieto, T. [Centre d' Études Nucléaire de Bordeaux-Gradignan, CNRS/IN2P3 – Université Bordeaux I, F-33175 Gradignan Cedex (France); Nilsson, T. [Fundamental Fysik, Chalmers Tekniska Högskola, S-41296 Göteborg (Sweden); Perea, A.; Pesudo, V. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); and others

    2014-10-07

    We present for the first time precise spectroscopic information on the recently discovered decay mode β-delayed 3p-emission. The detection of the 3p events gives an increased sensitivity to the high energy part of the Gamow–Teller strength distribution from the decay of {sup 31}Ar revealing that as much as 30% of the strength resides in the β3p-decay mode. A simplified description of how the main decay modes evolve as the excitation energy increases in {sup 31}Cl is provided.

  1. SOLVING THE STUDENTS’ SPEAKING PROBLEM IN DELIVERING ENGLISH PRESENTATION THROUGH 3-P TECHNIQUE

    Directory of Open Access Journals (Sweden)

    Nadif Ulfia

    2017-01-01

    Full Text Available Abstract: Solving the Students’ Speaking Problem in Delivering English Presentation Through 3-P Technique. The aims of this study were to (1 identify whether and to what extent 3-P Technique can improve the students’ speaking competence in delivering presentation;(2 describe the teaching and learning when 3-P technique is implemented in English class. This research was conducted at the eleventh grades of MAN 1 Bojonegoro. The subjects of the research were the teacher and the students in the academic year 2016/2017. This study applied 3 cycle action research. In collecting the data, the researcher used observations through field notes, video recording, questionnaire and interview. To analyze the quantitative data, the researcher applied descriptive statistics. Besides, to analyze qualitative data, the researcher applied constant comparative method as suggested by Garner. The findings shows that: (1 3-P Technique can improve students’ speaking competence in delivering presentation in terms of students achievement, fluency, pronunciation, ability to express their opinions, arguments, and ideas using appropriate vocabulary and grammatical form, using appropriate body language, voice control, gesture, and eye contact; and (2 3-P technique can improve classroom situation in terms of increasing students’ motivation and participation, creating live teaching atmosphere, and increasing teacher motivation. Key words: Speaking, 3 P technique, Action research Abstrak: Memecahkan Masalah Keterampilan Berbicara Siswa dalam Menyampaikan Presentasi Berbahasa Inggris Melalui Teknik 3-P. Tujuan dari penelitian ini adalah untuk (1 mengidentifikasi dan mengetahui sejauh mana teknik 3-P dapat meningkatkan kemampuan berbicara para siswa dalam menyampaikan sebuah presentasi; (2 mendeskripsikan kegiatan pembelajaran dalam penerapan teknik 3-P dalam pembelajaran Bahasa Inggris. Penelitian ini dilakukan pada kelas sebelas MAN 1 Bojonegoro. Subyek  penelitian ini

  2. The Application of 3P+MYSQL Technology of Dynamic Website Design%3P+MYSQL技术在动态网站设计中的运用

    Institute of Scientific and Technical Information of China (English)

    周悦; 朱小社

    2004-01-01

    在Windows2000 Server平台下分别利用ASP、PHP、JSP这三个动态网站技术,使用可以跨Windows和Linux平台的MYSQL数据库,采用三种连接数据库的方法,在三种不同特点的WEB服务器环境下,给出了动态网站设计中3P+MYSQL技术的运用.

  3. Isotope shifts of the (3s3p) 3P0,1,2-(3s4s) 3S1 Mg I transitions

    DEFF Research Database (Denmark)

    He, Ming; Therkildsen, Kasper T.; Jensen, Brian B.;

    2009-01-01

    We report measurements of the isotope shifts of the (3s3p)P30,1,2-(3s4s)S31 Mg I transitions for the stable isotopes M24g (I=0) , M25g (I=5/2) , and M26g (I=0) . Furthermore, the M25g S31 hyperfine coefficient A(S31)=(-321.6±1.5)MHz is extracted and found to be in excellent agreement with state...

  4. Pure partial monosomy 3p (3p25.3 → pter: Prenatal diagnosis and array comparative genomic hybridization characterization

    Directory of Open Access Journals (Sweden)

    Chih-Ping Chen

    2012-09-01

    Conclusion: In this case, aCGH has characterized a 3p deleted region with haploinsufficiency of the neurodevelopmental genes associated with cognitive deficit and mental retardation but without involvement of the congenital heart disease susceptibility locus, and QF-PCR has determined a paternal origin of the deletion. aCGH and QF-PCR help to delineate the genomic imbalance in prenatally detected de novo chromosome aberration, and the information acquired is useful for genetic counseling.

  5. Surface and interface properties of Zn3P2 solar cells

    Science.gov (United States)

    Kazmerski, L. L.; Ireland, P. J.; Catalano, A.

    1981-03-01

    The compositional properties of Zn3P2 thin films are studied using surface analysis techniques. Relative sensitivity factors of S(Zn sub LMM) = 0.29 and S(P sub LMM) = 0.34 are determined for this semiconductor with the aid of Auger electron spectroscopy standard spectra. The interfacial properties of the Mg/Zn3P2 thin-film solar cell are studied using Auger electron spectroscopy depth-compositional profiling in conjunction with complementary secondary ion mass spectroscopy and X-ray photoelectron spectroscopy data. Evidence is adduced for the formation of Mg3P2 at the metal-semiconductor interface. It is noted that this compound has a bandgap near that of Zn3P2. Attention is given to the possible formation of a heterojunction as a result of the interfacial reaction.

  6. 多通道反应O(3P)+CH2F的理论研究%Vibrational Mode Analysis for the Multi-channel Reactions of O(3P) with CH2F

    Institute of Scientific and Technical Information of China (English)

    曹小龙; 郭丽

    2004-01-01

    用密度泛函理论研究了氧原子与氟代甲基自由基的反应.反应中出现的所有物种的平衡构型用B3LYP方法在6-311++G(2 d,2p)基组水平上进行了优化,同时对各物种进行了频率分析;在同一理论水平上计算了各反应通道的势能面变化,分析了反应物、中间体、过渡态、产物的振动模式随反应途径的变化关系,阐明了该多通道反应的反应机理.%All species involved in the multi-channel reaction of an oxygen atom with a fluorinated methyl radical have been investigated using density functional theory (DFT) . The geometries of the reactants, intermediates, transition states and products are optimized at the B3LYP/6-311 + + G(2 d, 2p) level. The potential energy surface for this reaction is calculated at the same level of theory. Various possible production channels involved in the reaction of O(3 P) +CH2F are examined. The vibrational mode analysis is used to elucidate the relationship between the transition states, intermediates and the products. The extensive investigation shows that the reaction mechanism is reliable.

  7. Newly discovered breast cancer susceptibility loci on 3p24 and 17q23.2

    DEFF Research Database (Denmark)

    Ahmed, Shahana; Thomas, Gilles; Ghoussaini, Maya

    2009-01-01

    strong evidence for additional susceptibility loci on 3p (rs4973768: per-allele OR = 1.11, 95% CI = 1.08-1.13, P = 4.1 x 10(-23)) and 17q (rs6504950: per-allele OR = 0.95, 95% CI = 0.92-0.97, P = 1.4 x 10(-8)). Potential causative genes include SLC4A7 and NEK10 on 3p and COX11 on 17q....

  8. Determination of diffusion, reflection and deexcitation coefficients of metastable excited Ne(3P2) atom

    Science.gov (United States)

    Suzuki, S.; Itoh, H.

    2016-05-01

    The diffusion coefficient of the metastable excited Ne(3P2) atom in neon, the reflection coefficient of Ne(3P2) at the surface of an electrode and the rate coefficient of Ne(3P2) for collisional quenching by Ne(1S0) were determined from the gas pressure dependence of the effective lifetime of Ne(3P2). The effective lifetime of Ne(3P2) was measured from the transient current after turning off the Ultraviolet (UV) light in a Townsend discharge. The observed transient current waveform was analysed by solving the diffusion equation for the metastable excited Ne(3P2) atom using the third kind of boundary condition. The rate coefficient of Ne(3P2) for collisional quenching by Ne(1S0) and the reflection coefficient were determined by a nonspectroscopic method for the first time in neon to the best of our knowledge and were (3.2  ±  0.4)  ×  10-16 cm3 s-1 and 0.10  ±  0.04, respectively. The obtained diffusion coefficient at 1 Torr was 177  ±  17 cm2 s-1, which is consistent with the value reported by Dixon and Grant. Moreover, the present results are compared with the results of Phelps and were found to be in good agreement. We also discuss the deexcitation rate of Ne(3P2) at pressures of up to 60 Torr in comparison with previously reported values.

  9. Misregulation of Scm3p/HJURP Causes Chromosome Instability in Saccharomyces cerevisiae and Human Cells

    OpenAIRE

    Prashant K. Mishra; Wei-Chun Au; John S. Choy; P Henning Kuich; Baker, Richard E.; Foltz, Daniel R.; Basrai, Munira A.

    2011-01-01

    The kinetochore (centromeric DNA and associated proteins) is a key determinant for high fidelity chromosome transmission. Evolutionarily conserved Scm3p is an essential component of centromeric chromatin and is required for assembly and function of kinetochores in humans, fission yeast, and budding yeast. Overexpression of HJURP, the mammalian homolog of budding yeast Scm3p, has been observed in lung and breast cancers and is associated with poor prognosis; however, the physiological relevanc...

  10. Photoionization cross sections of the excited 3s3p 3Po state for atomic Mg

    Science.gov (United States)

    Wang, Guoli; Wan, Jianjie; Zhou, Xiaoxin

    2017-01-01

    The photoionization cross sections of the excited levels (3s3p 0,1,2,o 3P) of atomic Mg have been studied theoretically using both the nonrelativistic and fully relativistic R-matrix method. For the threshold cross sections, as previous nonrelativistic studies, present calculations show significant differences (a factor of 3) from former experimental values. Large discrepancies with experiment calls for additional measurements of the photoionization cross sections from the excited states of Mg.

  11. Clock-controlled mir-142-3p can target its activator, Bmal1

    Directory of Open Access Journals (Sweden)

    Tan Xiaochao

    2012-09-01

    Full Text Available Abstract Background microRNAs (miRNAs are shown to be involved in the regulation of circadian clock. However, it remains largely unknown whether miRNAs can regulate the core clock genes (Clock and Bmal1. Results In this study, we found that mir-142-3p directly targeted the 3’UTR of human BMAL1 and mouse Bmal1. The over-expression (in 293ET and NIH3T3 cells and knockdown (in U87MG cells of mir-142-3p reduced and up-regulated the Bmal1/BMAL1 mRNA and protein levels, respectively. Moreover, the expression level of mir-142-3p oscillated in serum-shocked NIH3T3 cells and the results of ChIP and luciferase reporter assays suggested that the expression of mir-142-3p was directly controlled by CLOCK/BMAL1 heterodimers in NIH3T3 cells. Conclusions Our study demonstrates that mir-142-3p can directly target the 3’UTR of Bmal1. In addition, the expression of mir-142-3p is controlled by CLOCK/BMAL1 heterodimers, suggesting a potential negative feedback loop consisting of the miRNAs and the core clock genes. These findings open new perspective for studying the molecular mechanism of circadian clock.

  12. MicroRNA-139-3p indicates a poor prognosis of colon cancer.

    Science.gov (United States)

    Liu, Xiaojing; Duan, Bensong; Dong, Yuanyuan; He, Chengzhi; Zhou, Hongmei; Sheng, Haihui; Gao, Hengjun; Zhang, Xizhi

    2014-01-01

    MicroRNAs (miRNAs) play an important role in the regulation of gene expression and are involved in almost biological procession. Recently, miR-139-5p has been reported to be downregulated in some types of cancer, and inhibits cancer cell invasion and metastasis. However, there are few reports on the role of miR-139-3p in cancer. In this study, we examined the expression level of miR-139-3p in 63 pairs of colon cancer and adjacent paracancerous tissues using quantitative reverse transcription PCR. The levels of miR-139-3p in colon cancer tissues were significantly lower than those in adjacent noncancerous tissues. There was an inverse correlation between the level of miR-139-3p and patient's age. Lower level of miR-139-3p was significantly associated with poor overall survival, especially in patients with TNM stages I and II. In conclusion, miR-139-3p has potential as a prognostic biomarker for colon cancer. Further prospective studies are required to validate this result.

  13. miR-579-3p controls melanoma progression and resistance to target therapy

    Science.gov (United States)

    Fattore, Luigi; Mancini, Rita; Acunzo, Mario; Romano, Giulia; Laganà, Alessandro; Pisanu, Maria Elena; Malpicci, Debora; Madonna, Gabriele; Mallardo, Domenico; Capone, Marilena; Fulciniti, Franco; Mazzucchelli, Luca; Botti, Gerardo; Croce, Carlo M.; Ascierto, Paolo Antonio; Ciliberto, Gennaro

    2016-01-01

    Therapy of melanoma patients harboring activating mutations in the BRAF (V-raf murine sarcoma viral oncogene homolog B1) oncogene with a combination of BRAF and MEK inhibitors is plagued by the development of drug resistance. Mutational events, as well as adaptive mechanisms, contribute to the development of drug resistance. In this context we uncover here the role of a miRNA, miR-579-3p. We first show that low expression of miR-579-3p is a negative prognostic factor correlating with poor survival. Expression levels of miR-579-3p decrease from nevi to stage III/IV melanoma samples and even further in cell lines resistant to BRAF/MEK inhibitors. Mechanistically, we demonstrate that miR-579-3p acts as an oncosuppressor by targeting the 3′UTR of two oncoproteins: BRAF and an E3 ubiquitin protein ligase, MDM2. Moreover miR-579-3p ectopic expression impairs the establishment of drug resistance in human melanoma cells. Finally, miR-579-3p is strongly down-regulated in matched tumor samples from patients before and after the development of resistance to targeted therapies. PMID:27503895

  14. Cu3P/RGO Nanocomposite as a New Anode for Lithium-Ion Batteries

    Science.gov (United States)

    Liu, Shuling; He, Xiaodong; Zhu, Jianping; Xu, Liqiang; Tong, Jianbo

    2016-10-01

    Cu3P/reduced graphene oxide (Cu3P/RGO) nanocomposite was successfully synthesized by a facile one-pot method as an advanced anode material for high-performance lithium-ion batteries. Cu3P nanostructures with a polyhedral shape with the mean diameter (80–100 nm) were homogeneously anchored on the surface of RGO. The flexible RGO sheets acted as elastic buffering layer which not only reduced the volume change, but also prevented the aggregation of Cu3P nanostructures, the cracking and crumbing of electrodes. On the other hand, the presence of Cu3P nanostructures could also avoid the agglomeration of RGO sheets and retain their highly active surface area. Therefore, as an advanced anode material for high-performance lithium-ion batteries, the as-prepared Cu3P/RGO exhibited high capacity of 756.15 mAhg‑1 at the current density 500 mAg‑1 after 80 cycles, superior cyclic stability and good rate capability.

  15. Genetic epidemiology of Paget's disease of bone in italy: sequestosome1/p62 gene mutational test and haplotype analysis at 5q35 in a large representative series of sporadic and familial Italian cases of Paget's disease of bone.

    Science.gov (United States)

    Falchetti, Alberto; Di Stefano, Marco; Marini, Francesca; Ortolani, Sergio; Ulivieri, Massimo Fabio; Bergui, Simona; Masi, Laura; Cepollaro, Chiara; Benucci, Maurizio; Di Munno, Ombretta; Rossini, Maurizio; Adami, Silvano; Del Puente, Antonio; Isaia, Giancarlo; Torricelli, Francesca; Brandi, Maria Luisa

    2009-01-01

    Families affected by Paget's disease of bone frequently harbor mutations in the SQSTM1/p62 gene. In this multicentric study we collected 345 sporadic and 12 familial PDB cases throughout Italy, identifying 12 different mutations, 5 of which are newly reported and 3, D335E, A381V, and Y383X, external to the UBA domain. Subjects with truncating mutations, E396X, showed a significantly younger age at clinical diagnosis, while the Y383X subjects had a higher average number of affected skeletal sites. All the mutants exhibited the CGTG-H2 haplotype. In two pairs and one triad of unrelated Italian PDB families from different Italian regions, we detected a common SQSTM1/p62 mutation for each P392L, M404V, and G425R group. Since the CGTG-H2 haplotype frequency was also high in normal subjects, and genetic influence due to migratory fluxes of different ethnic groups exists in the Italian population, to refine the search for a more geographically specific founder effect, we extended the haplotype analysis in these families using polymorphic microsatellite repeat markers, within and flanking the SQSTM1/p62 locus, from chromosome 5q35, other than the exon 6 and 3'UTR polymorphisms. All mutant carriers from two of the three M404V families and from the G425R families exhibited common extended chromosome 5q35 haplotypes, IT01 and IT02, respectively, which may be reflecting influences of past migrations. This may be helpful in estimating the true rate of de novo mutations. We confirm the data on the existence of both a mutational hotspot at the UBA domain of SQSTM1/p62 and a founder effect in the PDB population.

  16. Randomized Phase III Study of Lenalidomide Versus Placebo in RBC Transfusion-Dependent Patients With Lower-Risk Non-del(5q) Myelodysplastic Syndromes and Ineligible for or Refractory to Erythropoiesis-Stimulating Agents.

    Science.gov (United States)

    Santini, Valeria; Almeida, Antonio; Giagounidis, Aristoteles; Gröpper, Stefanie; Jonasova, Anna; Vey, Norbert; Mufti, Ghulam J; Buckstein, Rena; Mittelman, Moshe; Platzbecker, Uwe; Shpilberg, Ofer; Ram, Ron; Del Cañizo, Consuelo; Gattermann, Norbert; Ozawa, Keiya; Risueño, Alberto; MacBeth, Kyle J; Zhong, Jianhua; Séguy, Francis; Hoenekopp, Albert; Beach, C L; Fenaux, Pierre

    2016-09-01

    This international phase III, randomized, placebo-controlled, double-blind study assessed the efficacy and safety of lenalidomide in RBC transfusion-dependent patients with International Prognostic Scoring System lower-risk non-del(5q) myelodysplastic syndromes ineligible for or refractory to erythropoiesis-stimulating agents. In total, 239 patients were randomly assigned (2:1) to treatment with lenalidomide (n = 160) or placebo (n = 79) once per day (on 28-day cycles). The primary end point was the rate of RBC transfusion independence (TI) ≥ 8 weeks. Secondary end points were RBC-TI ≥ 24 weeks, duration of RBC-TI, erythroid response, health-related quality of life (HRQoL), and safety. RBC-TI ≥ 8 weeks was achieved in 26.9% and 2.5% of patients in the lenalidomide and placebo groups, respectively (P 500 mU/mL). At week 12, mean changes in HRQoL scores from baseline did not differ significantly between treatment groups, which suggests that lenalidomide did not adversely affect HRQoL. Achievement of RBC-TI ≥ 8 weeks was associated with significant improvements in HRQoL (P < .01). The most common treatment-emergent adverse events were neutropenia and thrombocytopenia. Lenalidomide yields sustained RBC-TI in 26.9% of RBC transfusion-dependent patients with lower-risk non-del(5q) myelodysplastic syndromes ineligible for or refractory to erythropoiesis-stimulating agents. Response to lenalidomide was associated with improved HRQoL. Treatment-emergent adverse event data were consistent with the known safety profile of lenalidomide. © 2016 by American Society of Clinical Oncology.

  17. Bonding and Site Preferences in the New Quasi-Binary Zr 2.7Hf 11.3P 9

    Science.gov (United States)

    Kleinke, Holger; Franzen, Hugo F.

    1998-03-01

    The new quasi-binary Zr 2.7Hf 11.3P 9was synthesized by arc-melting of Zr, Hf, Co, and HfP in a ratio corresponding to the initial composition "Zr 2.25Hf 6.75Co 2P 4". Zr 2.7Hf 11.3P 9crystallizes in the Zr 14P 9structure type, which is unknown in the binary Hf/P system. The ideal orthorhombic lattice dimensions (space group Pnnm(No. 58), Z=4) were refined to a=16.640(7) Å, b=27.40(2) Å, c=3.619(1) Å, V=1650(2) Å 3. The structure consists of three-dimensional condensed one-, two-, and three-capped trigonal (Zr, Hf) 6P prisms, occurring with numerous short M- Mbonds ( M=Zr, Hf). Each of the 15 metal sites is statistically occupied by a mixture of Zr and Hf, which varies significantly from site to site. The Hf/Zr ratio in a given site depends on the M- Mand M-P interactions. The systematic increase of this ratio with increasing total bond order, as evaluated via Mulliken overlap populations and Pauling bond orders, can be understood based on the trend that Hf forms stronger M- Mand M-P bonds than Zr. As expected for a metal-rich phosphide, band structure calculations for the hypothetical "Hf 14P 9" carried out with the extended Hückel approximation result in a significant density of states at the Fermi level.

  18. Effect of Fe2P in LiFePO4/Fe2P composite on electrochemical properties synthesized by MA and control of heat condition

    Institute of Scientific and Technical Information of China (English)

    PARK Jong Suk; LEE Kyung Tae; LEE Kyung Sub

    2006-01-01

    In order to control the size and distribution of the high conductive Fe2P in LiFePO4/Fe2P composite, two different cooling rates (Fast: 15 ℃·min-1, Slow: 2 ℃·min-1) were employed after mechanical alloying.The discharge capacity of the fast cooled was 83 mAh·g-1 and the slow cooled 121 mAh·g-1.The particle size of the synthesized powder was examined by transmission electron microscopy and distribution of Fe2P was characterized using scanning electron microscopy (SEM).In addition, two-step heat treatment was carried out for better distribution of Fe2P.X-ray diffraction (XRD) and Rietveld refinement reveal that LiFePO4/Fe2P composite consists of 95.77% LiFePO4 and 4.33% of Fe2P.

  19. Non-P2P network applicationautomatic feature recognition%非 P2P 网络应用自动化特征提取

    Institute of Scientific and Technical Information of China (English)

    丁斌; 焦素云; 闫晓亮

    2015-01-01

    在对流量进行分类的基础上,应用Apriori算法分析分类以后的非P2P流,能够更准确地分析出非P2 P的网络应用特征。%Based on the classified traffic flow between P2P and non‐P2P ,Apriori algorithm is used to analyzing the non‐P2P flow to get the high precise characteristics of non‐P2P network application .

  20. The Oscillator Strengths of H2+, 1s\\sigma_{g}-2p\\sigma_{u}, 1s\\sigma_{g}-2p\\pi_{u}

    CERN Document Server

    Tsogbayar, Ts

    2010-01-01

    The oscillator strengths of the $H^+_2$ molecular ion, $1s\\sigma_{g}-2p\\sigma_{u}$, $1s\\sigma_{g}-2p\\pi_{u}$ are calculated within the Born-Oppenheimer approximation. The variational expansion with randomly chosen exponents has been used for numerical studies. The oscillator strengths obtained for the transitions $1s\\sigma_{g}-2p\\sigma_{u}$, $1s\\sigma_{g}-2p\\pi_{u}$ of $H^+_2$ are accurate up to ten significant digits. Results are given for the internuclear distances between 0.10 and 20.0 a.u.

  1. Observation of the 2p3/2 -> 2s1/2 intra-shell transition in He-like uranium

    CERN Document Server

    Trassinelli, Martino; Beyer, Heinrich; Indelicato, Paul; Märtin, Renate; Reuschl, Regina; Kozhedub, Yuri S; Brandau, Carsten; Bräuning, Harald; Geyer, Sabrina; Gumberidze, Alexander; Hess, Sebastian; Jagodzinski, Pawel; Kozhuharov, Christophor; Liesen, Dieter; Trotsenko, Sergiy; Weber, Günter; Winters, Danyal; Stöhlker, Thomas

    2008-01-01

    We present the first identification and energy measurement of the 1s2p 3P2 \\to 1s2s 3S1 transition in He-like uranium. The experiment was performed at the internal gas-jet target of the ESR storage ring at GSI by means of a Bragg crystal spectrometer. Using the 1s2 2p 2P3/2 \\to 1s2 2s 2S1/2 transition in Li-like uranium as reference, we obtained an accuracy in the order of 0.01%, which provides, at present, the most accurate test of many-body and QED contributions in very heavy He-like ions

  2. Specific production of very long-lived core-excited sulfur atoms by 2p{sup -1}{sigma}* excitation of the OCS molecule followed by ultrafast dissociation

    Energy Technology Data Exchange (ETDEWEB)

    Fink, Reinhold F [Department of Physics, Uppsala University, Box 530, SE-751 21 Uppsala (Sweden); Eschner, Annika [Department of Synchrotron Radiation Research, Institute of Physics, University of Lund, Box 118, SE-221 00 Lund (Sweden); Magnuson, Martin [Department of Physics, Uppsala University, Box 530, SE-751 21 Uppsala (Sweden); Bjoerneholm, Olle [Department of Physics, Uppsala University, Box 530, SE-751 21 Uppsala (Sweden); Hjelte, Ingela [Department of Physics, Uppsala University, Box 530, SE-751 21 Uppsala (Sweden); Miron, Catalin [MAXLAB, University of Lund, Box 118, SE-221 00 Lund (Sweden); Bassler, Margit [MAXLAB, University of Lund, Box 118, SE-221 00 Lund (Sweden); Svensson, Svante [Department of Physics, Uppsala University, Box 530, SE-751 21 Uppsala (Sweden); Piancastelli, Maria Novella [Department of Physics, Uppsala University, Box 530, SE-751 21 Uppsala (Sweden); Sorensen, Stacey L [Department of Synchrotron Radiation Research, Institute of Physics, University of Lund, Box 118, SE-221 00 Lund (Sweden)

    2006-06-28

    A core-excited sulfur state with a lifetime almost one order of magnitude longer than in molecular 2p core-hole states is selectively produced by ultrafast dissociation of S 2p {yields} {sigma}* excited OCS. Clear evidence for this is provided by strong atomic peaks (20% of the total intensity) in x-ray fluorescence but very weak ones (2%) in the corresponding resonant Auger spectrum. Corroborating the assignment of the spectra, ab initio calculations explain the enhanced lifetime: the Auger decay of the produced {sup 3}D{sub 3} (2p{sup 5}3p{sup 5}) sulfur state is strongly decreased as it contradicts a newly derived propensity rule of the L{sub 2,3}MM Auger decay. (letter to the editor)

  3. Microduplications of 3p26.3p26.2 containing CRBN gene in patients with intellectual disability and behavior abnormalities.

    Science.gov (United States)

    Papuc, Sorina M; Hackmann, Karl; Andrieux, Joris; Vincent-Delorme, Catherine; Budişteanu, Magdalena; Arghir, Aurora; Schrock, Evelin; Ţuţulan-Cuniţă, Andreea C; Di Donato, Nataliya

    2015-05-01

    We report on the clinical data and molecular cytogenetic findings in three unrelated patients presenting with intellectual disability and behavior abnormalities. An overlapping microduplication involving 3p26.2-26.3 was identified in these patients. All three aberrations were confirmed and proven to be parentally inherited. The sizes of the duplications were different, with a common minimal region of 423,754 bp containing two genes - TRNT1 and CRBN. Here, we hypothesize that the copy number gain of CRBN gene might be responsible for developmental delay/intellectual disability.

  4. Genistein up-regulates tumor suppressor microRNA-574-3p in prostate cancer.

    Directory of Open Access Journals (Sweden)

    Takeshi Chiyomaru

    Full Text Available Genistein has been shown to inhibit cancers both in vitro and in vivo, by altering the expression of several microRNAs (miRNAs. In this study, we focused on tumor suppressor miRNAs regulated by genistein and investigated their function in prostate cancer (PCa and target pathways. Using miRNA microarray analysis and real-time RT-PCR we observed that miR-574-3p was significantly up-regulated in PCa cells treated with genistein compared with vehicle control. The expression of miR-574-3p was significantly lower in PCa cell lines and clinical PCa tissues compared with normal prostate cells (RWPE-1 and adjacent normal tissues. Low expression level of miR-574-3p was correlated with advanced tumor stage and higher Gleason score in PCa specimens. Re-expression of miR-574-3p in PCa cells significantly inhibited cell proliferation, migration and invasion in vitro and in vivo. miR-574-3p restoration induced apoptosis through reducing Bcl-xL and activating caspase-9 and caspase-3. Using GeneCodis software analysis, several pathways affected by miR-574-3p were identified, such as 'Pathways in cancer', 'Jak-STAT signaling pathway', and 'Wnt signaling pathway'. Luciferase reporter assays demonstrated that miR-574-3p directly binds to the 3' UTR of several target genes (such as RAC1, EGFR and EP300 that are components of 'Pathways in cancer'. Quantitative real-time PCR and Western analysis showed that the mRNA and protein expression levels of the three target genes in PCa cells were markedly down-regulated with miR-574-3p. Loss-of-function studies demonstrated that the three target genes significantly affect cell proliferation, migration and invasion in PCa cell lines. Our results show that genistein up-regulates tumor suppressor miR-574-3p expression targeting several cell signaling pathways. These findings enhance understanding of how genistein regulates with miRNA in PCa.

  5. BOREAS Level-3p Landsat TM Imagery: Geocoded and Scaled At-sensor Radiance

    Science.gov (United States)

    Nickeson, Jaime; Knapp, David; Newcomer, Jeffrey A.; Hall, Forrest G. (Editor); Cihlar, Josef

    2000-01-01

    For BOReal Ecosystem-Atmosphere Study (BOREAS), the level-3p Landsat Thematic Mapper (TM) data were used to supplement the level-3s Landsat TM products. Along with the other remotely sensed images, the Landsat TM images were collected in order to provide spatially extensive information over the primary study areas. This information includes radiant energy, detailed land cover, and biophysical parameter maps such as Fraction of Photosynthetically Active Radiation (FPAR) and Leaf Area Index (LAI). Although very similar to the level-3s Landsat TM products, the level-3p images were processed with ground control information, which improved the accuracy of the geographic coordinates provided. Geographically, the level-3p images cover the BOREAS Northern Study Area (NSA) and Southern Study Area (SSA). Temporally, the four images cover the period of 20-Aug-1988 to 07-Jun-1994. Except for the 07-Jun-1994 image, which contains seven bands, the other three contain only three bands.

  6. Magnetic Properties of Quantized Vortices in Neutron $^3P_2$ Superfluids in Neutron Stars

    CERN Document Server

    Masuda, Kota

    2015-01-01

    We discuss quantized vortices in neutron $^3P_2$ superfluids, which are believed to realize in high density neutron matter such as neutron stars. By using the Ginzburg-Landau free energy for $^3P_2$ superfluids, we determine the ground state in the absence and presence of the external magnetic field, and numerically construct $^3P_2$ quantized vortices in the absence and presence of the external magnetic field along the vortex axis (poloidal) or angular direction (toroidal). We find in certain situations the spontaneous magnetization of the vortex core, whose typical magnitude is about $10^{7-8}$ Gauss, but the net magnetic field in a neutron star is negligible because of the ratio of the vortex core size $\\sim 10$fm and the intervortex distance $\\sim 10^{-6}$m in a vortex lattice.

  7. A new form of Ca3P2 with a ring of Dirac nodes

    Directory of Open Access Journals (Sweden)

    Lilia S. Xie

    2015-08-01

    Full Text Available We report the synthesis and crystal structure of a new high-temperature form of Ca3P2. The crystal structure was determined through Rietveld refinements of synchrotron powder x-ray diffraction data. This form of Ca3P2 has a crystal structure of the hexagonal Mn5Si3 type, with a Ca ion deficiency compared to the ideal 5:3 stoichiometry. This yields a stable, charge-balanced compound of Ca2+ and P3−. We also report the observation of a secondary hydride phase, Ca5P3H, which again is a charge-balanced compound. The calculated band structure of Ca3P2 indicates that it is a three-dimensional Dirac semimetal with a highly unusual ring of Dirac nodes at the Fermi level. The Dirac states are protected against gap opening by a mirror plane in a manner analogous to what is seen for graphene.

  8. Experimental and theoretical study of 3p photoionization and subsequent Auger decay in atomic chromium

    Science.gov (United States)

    Keskinen, J.; Huttula, S.-M.; Mäkinen, A.; Patanen, M.; Huttula, M.

    2015-12-01

    3p photoionization and subsequent low kinetic energy Coster-Kronig and super Coster-Kronig Auger decay have been studied in atomic chromium. The binding energies, line widths, and relative intensities for the transitions seen in the synchrotron radiation excited 3p photoelectron spectrum are determined. The high resolution M2,3 M4,5 M4,5 and M2,3 M4,5 N1 Auger electron spectra following the electron impact excited 3p ionization are presented and the kinetic energies, relative intensities, and identifications are given for the main lines. The experimental findings are compared with the theoretical predictions obtained from Hartree-Fock and multiconfiguration Dirac-Fock approaches.

  9. Altered expression of two-pore domain potassium (K2P channels in cancer.

    Directory of Open Access Journals (Sweden)

    Sarah Williams

    Full Text Available Potassium channels have become a focus in cancer biology as they play roles in cell behaviours associated with cancer progression, including proliferation, migration and apoptosis. Two-pore domain (K2P potassium channels are background channels which enable the leak of potassium ions from cells. As these channels are open at rest they have a profound effect on cellular membrane potential and subsequently the electrical activity and behaviour of cells in which they are expressed. The K2P family of channels has 15 mammalian members and already 4 members of this family (K2P2.1, K2P3.1, K2P9.1, K2P5.1 have been implicated in cancer. Here we examine the expression of all 15 members of the K2P family of channels in a range of cancer types. This was achieved using the online cancer microarray database, Oncomine (www.oncomine.org. Each gene was examined across 20 cancer types, comparing mRNA expression in cancer to normal tissue. This analysis revealed all but 3 K2P family members (K2P4.1, K2P16.1, K2P18.1 show altered expression in cancer. Overexpression of K2P channels was observed in a range of cancers including breast, leukaemia and lung while more cancers (brain, colorectal, gastrointestinal, kidney, lung, melanoma, oesophageal showed underexpression of one or more channels. K2P1.1, K2P3.1, K2P12.1, were overexpressed in a range of cancers. While K2P1.1, K2P3.1, K2P5.1, K2P6.1, K2P7.1 and K2P10.1 showed significant underexpression across the cancer types examined. This analysis supports the view that specific K2P channels may play a role in cancer biology. Their altered expression together with their ability to impact the function of other ion channels and their sensitivity to environmental stimuli (pO2, pH, glucose, stretch makes understanding the role these channels play in cancer of key importance.

  10. Domain architectures of the Scm3p protein provide insights into centromere function and evolution

    OpenAIRE

    Aravind, L.; Lakshminarayan, M. Iyer; Wu, Carl

    2007-01-01

    Recently, Scm3p has been shown to be a nonhistone component of centromeric chromatin that binds stoichiometrically to CenH3–H4 histones, and to be required for the assembly of kinetochores in S. cerevisiae. Scm3p is conserved across fungi, and displays a remarkable variation in protein size, ranging from ~200 amino acids in Saccharomyces cerevisiae to ~1300 amino acids in Neurospora crassa. This is primarily due a variable C-terminal segment that is linked to a conserved N-terminal, CenH3-int...

  11. Autism spectrum disorder, Klinefelter syndrome, and chromosome 3p21.31 duplication: a case report.

    Science.gov (United States)

    Stuart, Scott W; King, Casey H; Pai, G Shashidar

    2007-12-18

    Autism spectrum disorders are heterogeneous in nature with idiopathic and genetic origins. We present a 7-year-old boy with a long history of multiple behavioral concerns, poor school performance, repetitive/compulsive tendencies, poor social skills, and language delays. A multidisciplinary evaluation concluded that the patient met full criteria for autism. A genetic evaluation demonstrated Klinefelter syndrome 47, XXY karyotype with concurrent duplication of 3p21.31 by microarray analysis. Maternal genetic analysis demonstrated the same 3p21.31 duplication. The potential implication with regard to autism spectrum disorders has not been previously discussed in the literature.

  12. Data of evolutionary structure change: 1Q95E-2P2GD [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1Q95E-2P2GD 1Q95 2P2G E D ANPLYQKHIISINDLSRDDLNLVLATAAKLKANP-QPEL...LKH-KVIASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDAIVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGD...RLDAMASVAT-VPVINALSD-EFHPCQVLADLQTIAERKGALRGLRLSYFGDGAN-NMAHSLLLGGVTAG-IHVTVAAPEGFLPDPSVRAAAERRAQDTGASVTVTAD...AHAAAAGADVLVTDTWV-----KPFRPF-----QLNSRLLALADSDAIVLHCLPAHRGDEITDAVMDGPASAVWDEAENRL.../pdbID> D 2P2GD RPLQG

  13. Music2Share - Copyright-Compliant Music Sharing in P2P Systems

    NARCIS (Netherlands)

    Kalker, Ton; Epema, Dick H.J.; Hartel, Pieter H.; Lagendijk, R. (Inald) L.; van Steen, Martinus Richardus; van Steen, Maarten

    2004-01-01

    Peer-to-Peer (P2P) networks are generally considered to be free havens for pirated content, in particular with respect to music. We describe a solution for the problem of copyright infringement in P2P networks for music sharing. In particular, we propose a P2P protocol that integrates the functions

  14. 3p14 De Novo Interstitial Microdeletion in a Patient with Intellectual Disability and Autistic Features with Language Impairment: A Comparison with Similar Cases

    Directory of Open Access Journals (Sweden)

    Ana Belén de la Hoz

    2015-01-01

    Full Text Available To date, few cases of 3p proximal interstitial deletions have been reported and the phenotype and genotype correlation is not well understood. Here, we report a new case of a 3p proximal interstitial deletion. The patient is an 11-year-old female with speech and social interaction difficulties, learning disability, and slight facial dysmorphism, but no other major malformations. An 8 Mb de novo interstitial deletion at 3p14.2-p14.1, from position 60.461.316 to 68.515.453, was revealed by means of array comparative genomic hybridization and confirmed using quantitative reverse-transcription polymerase chain reaction assays. This region includes six genes: FEZF2, CADPS, SYNPR, ATXN7, PRICKLE, and MAGI1, that are known to have a role in neurodevelopment. These genes are located on the proximal side of the deletion. We compare our case with previously well-defined patients reported in the literature and databases.

  15. The infinite order point on Y~2=X~3-p~3

    Institute of Scientific and Technical Information of China (English)

    张绍伟

    1995-01-01

    The parameterization of the elliptic curve Y2=X2+1 is given by using the modular forms of X(12) Then using class field theory over imaginary quadratic field and Shimura reciprocity, an infinite order point on the curve y2- = x3-p3 is constructed, for prime p=7 (mod 24).

  16. Using P3P in a web services-based context-aware application platform

    NARCIS (Netherlands)

    Zuidweg, M.; Halasz, E.; Goncalves Filho, J.; Lukovszki, C.; Marosits, T.; van Sinderen, Marten J.

    This paper describes a proposal for a privacy control architecture to be applied in the WASP project. The WASP project aims to develop a context-aware service platform on top of 3G networks, using web services technology. The proposed privacy control architecture is based on the P3P privacy policy

  17. MicroRNA-142-3p Negatively Regulates Canonical Wnt Signaling Pathway.

    Directory of Open Access Journals (Sweden)

    Tanyu Hu

    Full Text Available Wnt/β-catenin signaling pathway plays essential roles in mammalian development and tissue homeostasis. MicroRNAs (miRNAs are a class of regulators involved in modulating this pathway. In this study, we screened miRNAs regulating Wnt/β-catenin signaling by using a TopFlash based luciferase reporter. Surprisingly, we found that miR-142 inhibited Wnt/β-catenin signaling, which was inconsistent with a recent study showing that miR-142-3p targeted Adenomatous Polyposis Coli (APC to upregulate Wnt/β-catenin signaling. Due to the discordance, we elaborated experiments by using extensive mutagenesis, which demonstrated that the stem-loop structure was important for miR-142 to efficiently suppress Wnt/β-catenin signaling. Moreover, the inhibitory effect of miR-142 relies on miR-142-3p rather than miR-142-5p. Further, we found that miR-142-3p directly modulated translation of Ctnnb1 mRNA (encoding β-catenin through binding to its 3' untranslated region (3' UTR. Finally, miR-142 was able to repress cell cycle progression by inhibiting active Wnt/β-catenin signaling. Thus, our findings highlight the inhibitory role of miR-142-3p in Wnt/β-catenin signaling, which help to understand the complex regulation of Wnt/β-catenin signaling.

  18. Linkage of atopic dermatitis to chromosomes 4q22, 3p24 and 3q21

    DEFF Research Database (Denmark)

    Christensen, Ulla; Møller-Larsen, Steffen; Nyegaard, Mette

    2009-01-01

    Atopic dermatitis (AD) is a common, itchy skin disease of complex inheritance characterized by dermal and epidermal inflammation. The heritability is considerable and well documented. To date, four genome scans have examined the AD phenotype, showing replicated linkage at 3p26-22, 3q13-21 and 18q11...

  19. Baryonic {sup 3}P{sub 2} superfluidity under charged-pion condensation with {delta} isobar

    Energy Technology Data Exchange (ETDEWEB)

    Takatsuka, T.; Tamagaki, R. [Iwate Univ., Morioka, Iwate (Japan)

    1999-08-01

    We study the baryonic {sup 3}P{sup 2} superfluidity under charged-pion condensation with isobar ({delta}) degrees of freedom. After a remark on motivations of the present study, the outline of theoretical framework is briefly described, typical results of the superfluid critical temperature are shown, and the possibility of coexistence of the superfluid with charged-pion condensation is discussed. (author)

  20. Construction of a consistent YAC contig for human chromosome region 3p14.1

    NARCIS (Netherlands)

    Bardenheuer, W; Michaelis, S; Lux, A; Vieten, L; Brocker, F; Julicher, K; Willers, C; Siebert, R; Smith, DI; vanderHout, AH; Buys, C; Schutte, J; Opalka, B

    1996-01-01

    Chromosomal deletions and translocations of human chromosome region 3p14 are observed in various human malignancies and suggest the existence of a tumor suppressor gene locus within this region. Tumors most frequently affected by these aberrations are small-cell lung cancer and renal-cell carcinoma.

  1. Using P3P in a web services-based context-aware application platform

    NARCIS (Netherlands)

    Zuidweg, M.; Goncalves Filho, J.; Sinderen, van M.J.; Halasz, E.; Lukovszki, C.; Marosits, T.

    2003-01-01

    This paper describes a proposal for a privacy control architecture to be applied in the WASP project. The WASP project aims to develop a context-aware service platform on top of 3G networks, using web services technology. The proposed privacy control architecture is based on the P3P privacy policy d

  2. Repumping of ultracold strontium atoms using the ^3P2 - ^3D2 transition

    Science.gov (United States)

    Mickelson, P. G.; Martinez de Escobar, Y. N.; Traverso, A. J.; Killian, T. C.

    2008-05-01

    We discuss recent experiments involving ultracold strontium. Using a commercially-available 3 micron laser, we repump atoms out of the ^3P2 level via the ^3D2 state and gain almost a factor of 10 in the number of atoms in our system. This increase in the signal-to-noise ratio enables improved spectroscopy of strontium in our optical trap.

  3. miR-141-3p inhibits human stromal (mesenchymal) stem cell proliferation and differentiation

    DEFF Research Database (Denmark)

    Qiu, Weimin; Kassem, Moustapha

    2014-01-01

    Wnt signaling determines human stromal (mesenchymal) stem cell (hMSC) differentiation fate into the osteoblast or adipocyte lineage. microRNAs (miRNAs) are small RNA molecules of 21-25 nucleotides that regulate many aspects of osteoblast biology. Thus, we examined miRNAs regulated by Wnt signaling...... in hMSC. We identified miRNA (miR)-141-3p as a Wnt target which in turn inhibited Wnt signaling. Moreover, miR-141-3p inhibited hMSC proliferation by arresting cells at the G1 phase of the cell cycle. miR-141-3p inhibited osteoblast differentiation of hMSC as evidenced by reduced alkaline phosphatase...... activity, gene expression and in vitro mineralized matrix formation. Bioinformatic studies, Western blot analysis and 3'UTR reporter assay demonstrated that cell division cycle 25A (CDC25A) is a direct target of miR-141-3p. siRNA-mediated knock-down of CDC25A inhibited hMSC proliferation and osteoblast...

  4. Observation of the Forbidden Magnetic Dipole Transition 6{sup 2}P{sub ½} --> 7{sup 2}P{sub ½} in Atomic Thallium

    Science.gov (United States)

    Chu, S.

    1976-10-01

    A measurement of the 6{sup 2}P{sub ½} --> 7{sup 2}P{sub ½} forbidden magnetic dipole matrix element in atomic thallium is described. A pulsed, linearly polarized dye laser tuned to the transition frequency is used to excite the thallium vapor from the 6{sup 2}P{sub ½} ground state to the 7{sup 2}P{sub ½} excited state. Interference between the magnetic dipole M1 amplitude and a static electric field induced E1 amplitude results in an atomic polarization of the 7{sup 2}P{sub ½} state, and the subsequent circular polarization of 535 nm fluorescence. The circular polarization is seen to be proportional to / as expected, and measured for several transitions between hyperfine levels of the 6{sup 2}P{sub ½} and 7{sup 2}P{sub ½} states. The result is = -(2.11 +- 0.30) x 10{sup -5} parallel bar e parallel bar dirac constant/2mc, in agreement with theory.

  5. Phase equilibria in the Mo-Fe-P system at 800 °C and structure of ternary phosphide (Mo(1-x)Fe(x))3P (0.10 ≤ x ≤ 0.15).

    Science.gov (United States)

    Oliynyk, Anton O; Lomnytska, Yaroslava F; Dzevenko, Mariya V; Stoyko, Stanislav S; Mar, Arthur

    2013-01-18

    Construction of the isothermal section in the metal-rich portion (<67 atom % P) of the Mo-Fe-P phase diagram at 800 °C has led to the identification of two new ternary phases: (Mo(1-x)Fe(x))(2)P (x = 0.30-0.82) and (Mo(1-x)Fe(x))(3)P (x = 0.10-0.15). The occurrence of a Co(2)Si-type ternary phase (Mo(1-x)Fe(x))(2)P, which straddles the equiatomic composition MoFeP, is common to other ternary transition-metal phosphide systems. However, the ternary phase (Mo(1-x)Fe(x))(3)P is unusual because it is distinct from the binary phase Mo(3)P, notwithstanding their similar compositions and structures. The relationship has been clarified through single-crystal X-ray diffraction studies on Mo(3)P (α-V(3)S-type, space group I42m, a = 9.7925(11) Å, c = 4.8246(6) Å) and (Mo(0.85)Fe(0.15))(3)P (Ni(3)P-type, space group I4, a = 9.6982(8) Å, c = 4.7590(4) Å) at -100 °C. Representation in terms of nets containing fused triangles provides a pathway to transform these closely related structures through twisting. Band structure calculations support the adoption of these structure types and the site preference of Fe atoms. Electrical resistivity measurements on (Mo(0.85)Fe(0.15))(3)P reveal metallic behavior but no superconducting transition.

  6. Energetics and Dynamics of the Reactions of O(3P) with Dimethyl Methylphosphonate and Sarin

    Science.gov (United States)

    Conforti, Patrick F.; Braunstein, Matthew; Dodd, James A.

    2009-10-01

    Electronic structure and molecular dynamics calculations were performed on the reaction systems O(3P) + sarin and O(3P) + dimethyl methylphosphonate (DMMP), a sarin simulant. Transition state geometries, energies, and heats of reaction for the major reaction pathways were determined at several levels of theory, including AM1, B3LYP/6-311+G(d,p), and CBS-QB3. The major reaction pathways for both systems are similar and include H-atom abstraction, H-atom elimination, and methyl elimination, in rough order from low to high energy. The H-atom abstraction channels have fairly low barriers (˜10 kcal mol-1) and are close to thermoneutral, while the other channels have relatively high energy barriers (>40 kcal mol-1) and a wide range of reaction enthalpies. We have also found a two-step pathway leading to methyl elimination through O-atom attack on the phosphorus atom for DMMP and sarin. For sarin, the two-step methyl elimination pathway is significantly lower in energy than the single-step pathway. We also present results of O(3P) + sarin and O(3P) + DMMP reaction cross sections over a broad range of collision energies (2-10 km s-1 collision velocities) obtained using the direct dynamics method with an AM1 semiempirical potential. These excitation functions are intended as an approximate guide to future hyperthermal measurements, which to our knowledge have not yet examined either of these systems. The reaction barriers, reaction enthalpies, transition state structures, and excitation functions are generally similar for DMMP and sarin, with some moderate differences for methyl elimination energetics, which indicates DMMP will likely be a good substitute for sarin in many O(3P) chemical investigations.

  7. Genome-wide association analysis in East Asians identifies breast cancer susceptibility loci at 1q32.1, 5q14.3 and 15q26.1

    Science.gov (United States)

    Cai, Qiuyin; Zhang, Ben; Sung, Hyuna; Low, Siew-Kee; Kweon, Sun-Seog; Lu, Wei; Shi, Jiajun; Long, Jirong; Wen, Wanqing; Choi, Ji-Yeob; Noh, Dong-Young; Shen, Chen-Yang; Matsuo, Keitaro; Teo, Soo-Hwang; Kim, Mi Kyung; Khoo, Ui Soon; Iwasaki, Motoki; Hartman, Mikael; Takahashi, Atsushi; Ashikawa, Kyota; Matsuda, Koichi; Shin, Min-Ho; Park, Min Ho; Zheng, Ying; Xiang, Yong-Bing; Ji, Bu-Tian; Park, Sue K.; Wu, Pei-Ei; Hsiung, Chia-Ni; Ito, Hidemi; Kasuga, Yoshio; Kang, Peter; Mariapun, Shivaani; Ahn, Sei Hyun; Kang, Han Sung; Chan, Kelvin Y. K.; Man, Ellen P. S.; Iwata, Hiroji; Tsugane, Shoichiro; Miao, Hui; Liao, Jiemin; Nakamura, Yusuke; Kubo, Michiaki; Delahanty, Ryan J.; Zhang, Yanfeng; Li, Bingshan; Li, Chun; Gao, Yu-Tang; Shu, Xiao-Ou; Kang, Daehee; Zheng, Wei

    2014-01-01

    In a three-stage genome-wide association study among East Asian women including 22,780 cases and 24,181 controls, we identified three novel genetic loci associated with breast cancer risk, including rs4951011 at 1q32.1 (in intron 2 of the ZC3H11A gene, P = 8.82 × 10−9), rs10474352 at 5q14.3 (near the ARRDC3 gene, P = 1.67 × 10−9), and rs2290203 at 15q26.1 (in intron 14 of the PRC1 gene, P = 4.25 × 10−8). These associations were replicated in European-ancestry populations including 16,003 cases and 41,335 controls (P = 0.030, 0.004, and 0.010, respectively). Data from the ENCODE project suggest that variants rs4951011 and rs10474352 may be located in an enhancer region and transcription factor binding sites, respectively. This study provides additional insights into the genetics and biology of breast cancer. PMID:25038754

  8. Evidence for locus heterogeneity in acrocephalosyndactyly: a refined localization for the Saethre-Chotzen syndrome locus on distal chromosome 7p--and exclusion of Jackson-Weiss syndrome from craniosynostosis loci on 7p and 5q.

    Science.gov (United States)

    van Herwerden, L; Rose, C S; Reardon, W; Brueton, L A; Weissenbach, J; Malcolm, S; Winter, R M

    1994-04-01

    Craniosynostosis (premature fusion of the skull sutures) occurs as a clinically heterogeneous group of disorders, frequently involving digital abnormalities. We have previously provisionally assigned the gene for one such condition, Saethre-Chotzen syndrome (ACS III), to chromosome 7p. Linkage analysis is now reported between ACS III and dinucleotide repeat loci on distal 7p. The maximum lod scores, Zmax, were 5.57 at a recombination fraction of .05, with D7S488, and 4.74 at a recombination fraction of .05, with D7S493. Only weak linkage, not reaching significance, was found with distal markers (D7S513 and afm281vc9) and a proximal marker (D7S516). Multipoint analysis shows that the disease locus lies between D7S513 and D7S516. Analysis of individual recombinants shows that the most likely position is between D7S493 and D7S516. Linkage data in regard of Jackson-Weiss syndrome demonstrate that this autosomal dominant form of acrocephalosyndactyly does not map to the ACS III region on 7p or to the acrocephalosyndactyly locus on 5q (Boston type). These findings underline the genetic heterogeneity among the different clinical conditions manifesting with acrocephalosyndactyly.

  9. Evidence for locus heterogeneity in acrocephalosyndactyly: A refined localization for the Saethre-Chotzen syndrome locus on distal chromosome 7p-and exclusion of Jackson-Weiss syndrome from craniosynostosis loci on 7p and 5q

    Energy Technology Data Exchange (ETDEWEB)

    Herwerden, L. van; Rose, C.S.P.; Reardon, W.; Malcolm, S.; Winter, R.M. (Institute of Child Health, London (United Kingdom)); Brueton, L.A. (Northwick Park Hospital, Harrow (United Kingdom)); Weissenbach, J. (Human Genome Research Centre, Evry (France))

    1994-04-01

    Craniosynostosis (premature fusion of the skull sutures) occurs as a clinically heterogeneous group of disorders, frequently involving digital abnormalities. The authors have previously provisionally assigned the gene for one such condition, Saethre-Chotzen syndrome (ACS III), to chromosome 7p. Linkage analysis is now reported between ACS III and dinucleotide repeat loci on distal 7p. The maximum lod scores, Z[sub max], were 5.57 at a recombination fraction of .05, with D7S488, and 4.74 at a recombination fraction of .05, with D7S493. Only weak linkage, not reaching significance, was found with distal markers (D7S513 and afm281vc9) and a proximal marker (D7S516). Multipoint analysis shows that the disease locus lies between D7S513 and D7S516. Analysis of individual recombinants shows that the most likely position is between D7S493 and D7S516. Linkage data in regard of Jackson-Weiss syndrome demonstrate that this autosomal dominant form of acrocephalosyndactyly does not map to the ACS III region on 7p or to the acrocephalosyndactyly locus on 5q (Boston type). These findings underline the genetic heterogeneity among the different clinical conditions manifesting with acrocephalosyndactyly. 20 refs., 3 figs., 2 tabs.

  10. Evolutionary divergence of Ure2pA glutathione transferases in wood degrading fungi.

    Science.gov (United States)

    Roret, Thomas; Thuillier, Anne; Favier, Frédérique; Gelhaye, Eric; Didierjean, Claude; Morel-Rouhier, Mélanie

    2015-10-01

    The intracellular systems of detoxification are crucial for the survival of wood degrading fungi. Within these systems, glutathione transferases could play a major role since this family of enzymes is specifically extended in lignolytic fungi. In particular the Ure2p class represents one third of the total GST number in Phanerochaete chrysosporium. These proteins have been phylogenetically split into two subclasses called Ure2pA and Ure2pB. Ure2pB can be classified as Nu GSTs because of shared structural and functional features with previously characterized bacterial isoforms. Ure2pA can rather be qualified as Nu-like GSTs since they exhibit a number of differences. Ure2pA possess a classical transferase activity, a more divergent catalytic site and a higher structural flexibility for some of them, compared to Nu GSTs. The characterization of four members of this Ure2pA subclass (PcUre2pA4, PcUre2pA5, PcUre2pA6 and PcUre2pA8) revealed specific functional and structural features, suggesting that these enzymes have rapidly evolved and differentiated, probably to adapt to the complex chemical environment associated with wood decomposition.

  11. An agent-based incentive mechanism for P2P systems

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jian-feng; WANG Ru-chuan

    2006-01-01

    With the increasing uses of peer-to-peer (P2P)systems, the problems of fair information and resources sharing become serious. P2P systems are self-organizing,distributed systems with no centralized authority, such as Free-riding and Tragedy of Commons. Because of the voluntary participation and lack of motivation, the information and resources available in P2P networks are extremely variable and unpredictable. This article studied the problems existing in P2P networks and propose a different method to stimulate the peers in P2P networks to share information and resources,using agents so as to improve the performance of P2P networks.And through simulation experiments and analyzing the results,it can be concluded that this mechanism can effectively solve the problems of fair sharing in P2P systems.

  12. Hollow Co2P nanoflowers organized by nanorods for ultralong cycle-life supercapacitors

    KAUST Repository

    Cheng, Ming

    2017-08-24

    Hollow Co2P nanoflowers (Co2P HNF) are successfully prepared via a one-step, template-free method. Microstructure analysis reveals that Co2P HNF is assembled by nanorods, possesses abundant mesopores and a amorphous carbon shell. Density functional theory calculation and electrochemical measurements demonstrate the high electrical conductivity of Co2P. Benefiting from the unique nanostructures, when employed as electrode material for supercapacitors, Co2P HNF exhibits a high specific capacitance, an outstanding rate capability, and an ultralong cycle stability. Furthermore,. the constructed Co2P HNF//AC ASC yields a high energy density of 30.5 Wh kg-1 at a power density of 850 W kg-1, along with an superior cycling performance (108.0% specific capacitance retained after 10000 cycles at 5 A g-1). These impressive results make Co2P HNF a promising candidate for supercapacitor applications.

  13. Forbidden 2 P- nP and 2 P- nF transitions in the energy spectrum of ultracold Rydberg lithium-7 atoms

    Science.gov (United States)

    Zelener, B. B.; Saakyan, S. A.; Sautenkov, V. A.; Manykin, E. A.; Zelener, B. V.; Fortov, V. E.

    2016-04-01

    Forbidden 2 P- nP and 2 P- nF transitions in the ranges of the principal quantum number n = 42-114 and n = 38-48 have been detected in the optical spectra of ultracold highly excited lithium-7 atoms. The presence of forbidden transitions is due to induced external electric fields. The quantum defects and ionization energy obtained in various experiments and predicted theoretically have been discussed.

  14. Photoelectric characteristics of CH3NH3PbI3/p-Si heterojunction

    Science.gov (United States)

    Yamei, Wu; Ruixia, Yang; Hanmin, Tian; Shuai, Chen

    2016-05-01

    Organic-inorganic hybrid perovskite CH3NH3PbI3 film is prepared on p-type silicon substrate using the one-step solution method to form a CH3NH3PbI3/p-Si heterojunction. The film morphology and structure are characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The photoelectric properties of the CH3NH3PbI3/p-Si heterojunction are studied by testing the current-voltage (I-V) with and without illumination and capacitance-voltage (C-V) characteristics. It turns out from the I-V curve without illumination that the CH3NH3PbI3/p-Si heterojunction has a rectifier feature with the rectification ratio over 70 at the bias of ±5 V. Also, there appears a photoelectric conversion phenomenon on this heterojunction with a short circuit current (Isc) of 0.16 μA and an open circuit voltage (Voc) of about 10 mV The high frequency C-V characteristic of the Ag/CH3NH3PbI3/p-Si heterojunction turns out to be similar to that of the metal-insulator-semiconductor (MIS) structure, and a parallel translation of the C-V curve along the forward voltage axis is found. This parallel translation means the existence of defects at the CH3NH3PbI3/p-Si interface and positive fixed charges in the CH3NH3PbI3 layer. The defects at the interface of the CH3NH3PbI3/p-Si heterojunction result in the dramatic decline of the Voc. Besides, the C-V test of CH3NH3PbI3 film shows a non-linear dielectric property and the dielectric value is about 4.64 as calculated. Project supported by the Hebei Province Natural Science Foundation of China (No. F2014202184) and the Tianjin Natural Science Foundation of China (No. 15JCZDJC37800).

  15. Interstitial deletion 2p11.2-p12: report of a patient with mental retardation and review of the literature.

    Science.gov (United States)

    Tzschach, Andreas; Graul-Neumann, Luitgard M; Konrat, Kateryna; Richter, Reyk; Ebert, Grit; Ullmann, Reinhard; Neitzel, Heidemarie

    2009-02-01

    Deletions of chromosome bands 2p11.2 and 2p12 are rare, and only six patients have been reported to date. Here, we report on a 5-year-old girl with an 11.4 Mb interstitial deletion of chromosome bands 2p11.2-p12 and the characterization of this deletion by high-resolution array CGH. The patient presented with mental retardation, microcephaly and short stature. Facial features included broad nasal bridge, frontal bossing and mild dolichocephaly. Phenotypic comparison with previously published patients failed to reveal a consistent clinical pattern apart from developmental delay/mental retardation, which is probably due to different sizes and/or positions of the individual deletions. Among the 40 known genes deleted in our patient is REEP1, haploinsufficiency of which causes autosomal dominant spastic paraplegia type 31 (SPG31, OMIM 610250). Additional patients with well-characterized deletions within 2p11.2 and 2p12 will be needed to determine the role of individual genes for the clinical manifestations. (c) 2009 Wiley-Liss, Inc.

  16. Control of sigma virus multiplication by the ref(2)P gene of Drosophila melanogaster: an in vivo study of the PB1 domain of Ref(2)P.

    Science.gov (United States)

    Carré-Mlouka, A; Gaumer, S; Gay, P; Petitjean, A M; Coulondre, C; Dru, P; Bras, F; Dezélée, S; Contamine, D

    2007-05-01

    Ref(2)P has been described as one of the Drosophila proteins that interacts with the sigma virus cycle. We generated alleles to identify critical residues involved in the restrictive (inhibiting viral multiplication) or permissive (allowing viral multiplication) character of Ref(2)P. We demonstrate that permissive alleles increase the ability of the sigma virus to infect Drosophila when compared to null alleles and we confirm that restrictive alleles decrease this capacity. Moreover, we have created alleles unfunctional in viral cycling while functional for Ref(2)P fly functions. This type of allele had never been observed before and shows that fly- and virus-related activities of Ref(2)P are separable. The viral status of Ref(2)P variants is determined by the amino-terminal PB1 domain polymorphism. In addition, an isolated PB1 domain mimics virus-related functions even if it is similar to a loss of function toward fly-related activities. The evolutionary tree of the Ref(2)P PB1 domain that we could build on the basis of the natural allele sequences is in agreement with an evolution of PB1 domain due to successive transient selection waves.

  17. 非结构化P2P Overlay拓扑结构分析%Analysis of Topology Structure in Unstructured P2P Overlay

    Institute of Scientific and Technical Information of China (English)

    姜卫; 王观玉; 宋世延

    2010-01-01

    一个合理的P2P overlay可以为P2P应用提供更好的支持.非结构化P2P overlay的构建比较简单随意,适合于信息发布、即时通讯等节点随时加入退出的情况.构建具有可扩展性和稳健性的网络拓扑,是非结构化P2P overlay的研究热点之一.文章首先介绍了几种典型的非结构化P2P overlay,然后与以往研究不同,通过GT-ITM建模分析了每种P2P overlay拓扑结构的性能,主要分析了不同拓扑结构节点的度、最短路径分布、节点发现概率及结构的健壮性等,该工作对构造合理的P2P overlay拓扑结构和P2P系统的研究具有重要意义.

  18. Multi-configuration Dirac-Hartree-Fock calculations of the transition rates of 2s22p2 - 2s2p3 and 2s2p3 - 2s22pnl (n ≥ 3) E1 transitions of N+

    Science.gov (United States)

    Shen, Xiaozhi; Liu, Juan; Zhou, Fuyang

    2016-10-01

    Wavefunctions were determined using the multi-configuration Dirac-Hartree-Fock method. The core-core, core-valence, valence correlation, Breit interaction and quantum electrodynamics effects, as well as some higher-order correlation effects, were considered to obtain accurate wavelengths (λ), oscillator strengths (gf) and transition rates (A) of 2s22p2 - 2s2p3, 2s2p3 - 2s22pnl (n ≥ 3) and 2s2p3 - 2s2p23s E1 transitions. The branching ratio of 2s2p3 5S^o_2 (namely Aλ2143.45/Aλ2139.68) based on the latest calculation of 2.462 ± 0.119 is recommended for the determination of a nebula's electron temperature and electron density. The largest calculated gf value of 2s2p3 - 2s22p4p is λ630.65, differing from that of λ1060.2 (i.e. 2s2p3 3P^o_2 - 2s22p4p 3S1) that was observed with the largest intensities in the Orion Nebula spectrum. In addition, the energy levels and the splittings of 2s2p3, the extremely difficult calculations of the rates of two-electron one-photon transitions as well as those of the very small intercombination A of 2s2p3 5S^o_2 were studied in detail. Because of the weak spin-orbit interaction, accurately calculating the levels 3P^o_{1,2,0} (or 3D^o_{3,2,1}) and their transition matrix elements is very sensitive to relativistic and electron correlation effects. A special case for this is when the transition operators synchronously applied to wavefunctions with regard to 2s2p3 3Po and 2s22pnl (n = 4) become extremely sensitive to some higher-order correlation effects.

  19. Mechanism and kinetics for the reaction of O(3P) with DMSO: A theoretical study

    Science.gov (United States)

    Mandal, Debasish; Bagchi, Sabyasachi; Das, Abhijit K.

    2012-11-01

    Mechanism and kinetics for the reaction of DMSO with O(3P) have been investigated by M06-2X/MG3S, CBS-QB3 and G4MP2 methods. Four possible reaction pathways are identified. Among them, the O(3P) addition to S-atom followed by CH3 elimination is almost exclusive. Four pre-reactive complexes have been located. AIM theory is used to determine the nature of interactions in these complexes. Considering the formation of pre-reactive complex, the rate constant for major pathway is calculated using transition state theory applied to a two-step mechanism. Enthalpies of formation at 298.15 K (ΔfH°298.15) have been calculated using the composite CBS-QB3, G4MP2 and G3B3 methods.

  20. Verifier-based Password Authenticated 3P-EKE Protocol using PCLA keys

    Directory of Open Access Journals (Sweden)

    Archana Raghuvamshi

    2016-06-01

    Full Text Available This paper endeavors to present a novel framework for the generic structure of a verifier-based password authenticated Three-Party Encrypted Key Exchange (3P-EKE protocol which yields more efficient protocol than the ones knew before. A previous framework presented by Archana and Premchand is more secured against all types of attacks like password guessing, replay, pre-play, man-in-the-middle attack etc. But unfortunately, this protocol does not solve the problem of a server compromise. These proofs help as inspiration to search for another framework. The framework we offer produces more efficient 3P-EKE protocol, and, in addition, delivers perceptive clarification about the existing attacks that do not solve in the previous framework. Moreover, it allows direct change from a class of verge private-key encryption to a hybrid (symmetric & Asymmetric one without significant overhead.

  1. Multi-reassortant G3P[3] group A rotavirus in a horseshoe bat in Zambia.

    Science.gov (United States)

    Sasaki, Michihito; Orba, Yasuko; Sasaki, Satoko; Gonzalez, Gabriel; Ishii, Akihiro; Hang'ombe, Bernard M; Mweene, Aaron S; Ito, Kimihito; Sawa, Hirofumi

    2016-10-01

    Group A rotavirus is a major cause of diarrhoea in humans, especially in young children. Bats also harbour group A rotaviruses, but the genetic backgrounds of bat rotavirus strains are usually distinct from those of human rotavirus strains. We identified a new strain of group A rotavirus in the intestinal contents of a horseshoe bat in Zambia. Whole genome sequencing revealed that the identified virus, named RVA/Bat-wt/ZMB/LUS12-14/2012/G3P[3], possessed the genotype constellation G3-P[3]-I3-R2-C2-M3-A9-N2-T3-E2-H3. Several genome segments of LUS12-14 were highly similar to those of group A rotaviruses identified from humans, cows and antelopes, indicating interspecies transmission of rotaviruses between bats and other mammals with possible multiple genomic reassortment events.

  2. The Participative Design of an Endoscopy Facility using Lean 3P.

    Science.gov (United States)

    Smith, Iain

    2016-01-01

    In the UK, bowel cancer is the second largest cancer killer. Diagnosing people earlier can save lives but demand for endoscopies is increasing and this can put pressure on waiting times. To address this challenge, an endoscopy unit in North East England decided to improve their facilities to increase capacity and create environments that improve the experience of users. This presented a significant opportunity for step change improvement but also a problem in terms of creating designs that meet user requirements whilst addressing structural or space constraints. The Lean design process known as '3P' (standing for the production preparation process) was utilised as a participative design strategy to engage stakeholders in the design of the new department. This involved a time-out workshop (or 3P event) in which Lean and participative design tools were utilised to create an innovative design based on 'point of delivery' (POD) principles. The team created a design that demonstrated an increase in treatment room capacity by 25% and bed capacity by 70% whilst reducing travel distance for patients by 25.8% and staff by 27.1%. This was achieved with an increase in available space of only 13%. The Lean 3P method provided a structured approach for corporate and clinical staff to work together with patient representatives as cross-functional teams. This participative approach facilitated communication and learning between stakeholders about care processes and personal preferences. Lean 3P therefore appears to be a promising approach to improving the healthcare facilities design process to meet user requirements.

  3. Temperature dependence and mechanism of the reaction between O(3P) and chlorine dioxide

    Science.gov (United States)

    Colussi, A. J.; Sander, S. P.; Fiedl, R. R.

    1992-01-01

    Second-order rate constants for the decay of O(3P) in excess chlorine dioxide, k(II), were measured as a function of total pressure (20-600 Torr argon) and temperature (248-312 K), using flash photolysis-atomic resonance fluorescence. Results indicate that k(II) is pressure dependent with a value, K(b), that is nonzero at zero pressure, and both the third-order rate constant and k(b) have negative temperature dependences.

  4. LiNbO3/p+n diode surface acoustic wave memory correlator

    Institute of Scientific and Technical Information of China (English)

    张朝; 水永安; 印建华

    1997-01-01

    A detailed theoretical analysis of strip-coupled LiNbO3/p+ n diode surface acoustic wave (SAW) memory correlator in the parametric mode is presented. The influence of some important factors on correlation output is analyzed and calculated, including the amplitudes of reference, read and write signal, duration of write signal and doping density of the diode array. The conclusions can be employed for the design of improved strip-coupled SAW memorycorrelators.

  5. High-energy single diffractive dissociation of nucleons and the 3P-model applicability range

    Energy Technology Data Exchange (ETDEWEB)

    Godizov, A.A., E-mail: anton.godizov@gmail.com

    2016-11-15

    The adequacy of the triple-Pomeron interaction approximation (the 3P-model) for description of the high-energy single diffractive dissociation of nucleons is analyzed via application to the available experimental data on nucleon–nucleon scattering, including the recent results produced by CMS Collaboration which allow to estimate reliably the triple-Pomeron coupling value. It is argued that the total contribution of secondary Reggeon exchanges is not negligible up to the Tevatron energy.

  6. Operationalising the Lean principles in maternity service design using 3P methodology

    Science.gov (United States)

    Smith, Iain

    2016-01-01

    The last half century has seen significant changes to Maternity services in England. Though rates of maternal and infant mortality have fallen to very low levels, this has been achieved largely through hospital admission. It has been argued that maternity services may have become over-medicalised and service users have expressed a preference for more personalised care. NHS England's national strategy sets out a vision for a modern maternity service that continues to deliver safe care whilst also adopting the principles of personalisation. Therefore, there is a need to develop maternity services that balance safety with personal choice. To address this challenge, a maternity unit in North East England considered improving their service through refurbishment or building new facilities. Using a design process known as the production preparation process (or 3P), the Lean principles of understanding user value, mapping value-streams, creating flow, developing pull processes and continuous improvement were applied to the design of a new maternity department. Multiple stakeholders were engaged in the design through participation in a time-out (3P) workshop in which an innovative pathway and facility for maternity services were co-designed. The team created a hybrid model that they described as “wrap around care” in which the Lean concept of pull was applied to create a service and facility design in which expectant mothers were put at the centre of care with clinicians, skills, equipment and supplies drawn towards them in line with acuity changes as needed. Applying the Lean principles using the 3P method helped stakeholders to create an innovative design in line with the aspirations and objectives of the National Maternity Review. The case provides a practical example of stakeholders applying the Lean principles to maternity services and demonstrates the potential applicability of the Lean 3P approach to design healthcare services in line with policy requirements

  7. Fracture toughness of Al-4%Mg/Al{sub 2}O{sub 3}p composites

    Energy Technology Data Exchange (ETDEWEB)

    Pestes, R.H. [Hewlett-Packard, Camus, WA 98607 (United States); Kamat, S.V. [Mechanical and Materials Engineering Department, Washington State University, Pullman, WA 99164-2920 (United States); Hirth, J.P. [Mechanical and Materials Engineering Department, Washington State University, Pullman, WA 99164-2920 (United States)

    1994-12-20

    Fracture toughness tests were carried out on Al-4%Mg/Al{sub 2}O{sub 3}p composites containing different sizes and volume fractions of alumina particulates. The results indicated that the fracture toughness was dependent on the interparticle spacing provided the particulate size was below a critical size. The critical particle size corresponds to the situation wherein the energy release rate on particulate cracking is sufficient to trigger dynamic unstable crack extension. ((orig.))

  8. Operationalising the Lean principles in maternity service design using 3P methodology.

    Science.gov (United States)

    Smith, Iain

    2016-01-01

    The last half century has seen significant changes to Maternity services in England. Though rates of maternal and infant mortality have fallen to very low levels, this has been achieved largely through hospital admission. It has been argued that maternity services may have become over-medicalised and service users have expressed a preference for more personalised care. NHS England's national strategy sets out a vision for a modern maternity service that continues to deliver safe care whilst also adopting the principles of personalisation. Therefore, there is a need to develop maternity services that balance safety with personal choice. To address this challenge, a maternity unit in North East England considered improving their service through refurbishment or building new facilities. Using a design process known as the production preparation process (or 3P), the Lean principles of understanding user value, mapping value-streams, creating flow, developing pull processes and continuous improvement were applied to the design of a new maternity department. Multiple stakeholders were engaged in the design through participation in a time-out (3P) workshop in which an innovative pathway and facility for maternity services were co-designed. The team created a hybrid model that they described as "wrap around care" in which the Lean concept of pull was applied to create a service and facility design in which expectant mothers were put at the centre of care with clinicians, skills, equipment and supplies drawn towards them in line with acuity changes as needed. Applying the Lean principles using the 3P method helped stakeholders to create an innovative design in line with the aspirations and objectives of the National Maternity Review. The case provides a practical example of stakeholders applying the Lean principles to maternity services and demonstrates the potential applicability of the Lean 3P approach to design healthcare services in line with policy requirements.

  9. $^3P_2$-$^3F_2$ Pairing in Dense Neutron Matter The Spectrum of Solutions

    CERN Document Server

    Zverev, M V; Khodel, V A

    2003-01-01

    The $^3P_2$-$^3F_2$ pairing model is generally considered to provide an adequate description of the superfluid states of neutron matter at densities some 2-3 times that of saturated symmetrical nuclear matter. The problem of solving the system of BCS gap equations expressing the $^3P_2$-$^3F_2$ model is attacked with the aid of the separation approach. This method, developed originally for quantitative study of S-wave pairing in the presence of strong short-range repulsions, serves effectively to reduce the coupled, singular, nonlinear BCS integral equations to a set of coupled algebraic equations. For the first time, sufficient precision becomes accessible to resolve small energy splittings between the different pairing states. Adopting a perturbative strategy, we are able to identify and characterize the full repertoire of real solutions of the $^3P_2$-$^3F_2$ pairing model, in the limiting regime of small tensor-coupling strength. The P-F channel coupling is seen to lift the striking parametric degeneracie...

  10. miR-142-3p prevents macrophage differentiation during cancer-induced myelopoiesis.

    Science.gov (United States)

    Sonda, Nada; Simonato, Francesca; Peranzoni, Elisa; Calì, Bianca; Bortoluzzi, Stefania; Bisognin, Andrea; Wang, Ena; Marincola, Francesco M; Naldini, Luigi; Gentner, Bernhard; Trautwein, Christian; Sackett, Sara Dutton; Zanovello, Paola; Molon, Barbara; Bronte, Vincenzo

    2013-06-27

    Tumor progression is accompanied by an altered myelopoiesis causing the accumulation of immunosuppressive cells. Here, we showed that miR-142-3p downregulation promoted macrophage differentiation and determined the acquisition of their immunosuppressive function in tumor. Tumor-released cytokines signaling through gp130, the common subunit of the interleukin-6 cytokine receptor family, induced the LAP∗ isoform of C/EBPβ transcription factor, promoting macrophage generation. miR-142-3p downregulated gp130 by canonical binding to its messenger RNA (mRNA) 3' UTR and repressed C/EBPβ LAP∗ by noncanonical binding to its 5' mRNA coding sequence. Enforced miR expression impaired macrophage differentiation both in vitro and in vivo. Mice constitutively expressing miR-142-3p in the bone marrow showed a marked increase in survival following immunotherapy with tumor-specific T lymphocytes. By modulating a specific miR in bone marrow precursors, we thus demonstrated the feasibility of altering tumor-induced macrophage differentiation as a potent tool to improve the efficacy of cancer immunotherapy.

  11. Study of the Ne(^3P_2) + CH_3F Electron Transfer Reaction below 1 Kelvin

    CERN Document Server

    Jankunas, Justin; Osterwalder, Andreas

    2014-01-01

    Relatively little is known about the dynamics of electron transfer reactions at low collision energy. We present a study of Penning ionization of ground state methyl fluoride molecules by electronically excited neon atoms in the 13 $\\mu$eV--4.8 meV (150 mK--56 K) collision energy range, using a neutral-neutral merged beam setup. Relative cross sections have been measured for three Ne($^3P_2$)+ CH$_3$F reaction channels by counting the number of CH$_3$F$^+$, CH$_2$F$^+$, and CH$_3^+$ product ions, as a function of relative velocity between the neon and methyl fluoride molecular beams. Experimental cross sections markedly deviate from the Langevin capture model at collision energies above 20 K. The branching ratios are constant. In other words, the chemical shape of the CH$_3$F molecule, as seen by Ne($^3P_2$) atom, appears not to change as the collision energy is varied, in contrast to related Ne($^3P_J$) + CH$_3$X (X=Cl and Br) reactions at higher collision energies.

  12. Effects of the electron correlation and Breit and hyperfine interactions on the lifetime of the 2p$^5$3s states in neutral neon

    CERN Document Server

    Li, Jiguang; Godefroid, Michel; Gaigalas, Gediminas

    2012-01-01

    In the framework of the multiconfiguration Dirac-Hartree-Fock method, we investigate the transition properties of four excited states in the $2p^53s$ configuration of neutral neon. The electron correlation effects are taken into account systematically by using the active space approach. The effect of higher-order correlation on fine structures is shown. We also study the influence of the Breit interaction and find that it reduces the oscillator strength of the $^3P^o_1 - ^1S_0$ transition by 17%. It turns out that the inclusion of the Breit interaction is essential even for such a light atomic system. Our ab initio calculated line strengths, oscillator strengths and transition rates are compared with other theoretical values and experimental measurements. Good agreement is found except for the $^3P^o_2 - ^1S_0$ M2 transition for which discrepancies of around 15% between theories and experiments remain. In addition, the impact of hyperfine interactions on the lifetimes of the $^3P^o_0$ and $^3P^o_2$ metastable...

  13. miR-409-3p suppresses breast cancer cell growth and invasion by targeting Akt1

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Guoqiang [Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan 250012 (China); Department of Thyroid and Breast Surgery, Hospital Affiliated to Binzhou Medical University, 661 Second Huanghe Street, Binzhou 256603 (China); Liu, Zengyan [Department of Hematology, Hospital Affiliated to Binzhou Medical University, 661 Second Huanghe Street, Binzhou 256603 (China); Xu, Hao [Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029 (China); Yang, Qifeng, E-mail: qifengy_sdu1@163.com [Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan 250012 (China)

    2016-01-08

    Altered levels and functions of microRNAs (miRNAs) are correlated with carcinogenesis. While miR-409-3p has been shown to play important roles in several cancer types, its function in the context of breast cancer (BC) remains unknown. In this study, miR-409-3p was significantly downregulated in BC tissues and cell lines, compared with the corresponding control counterparts. Overexpression of miR-409-3p inhibited BC cell proliferation, migration and invasion in vitro and suppressed tumor growth in vivo. Notably, miR-409-3p induced downregulation of Akt1 protein through binding to its 3′ untranslated region (UTR). Conversely, restoring Akt1 expression rescued the suppressive effects of miR-409-3p. Our data collectively indicate that miR-409-3p functions as a tumor suppressor in BC through downregulating Akt1, supporting the targeting of the novel miR-409-3p/Akt1 axis as a potentially effective therapeutic approach for BC. - Highlights: • miR-409-3p inhibits proliferation, migration and invasion of BC cells. • miR-409-3p suppresses tumor growth in nude mice. • Akt1 is a direct downstream target of miR-409-3p. • Ectopic expression of Akt1 reverses the effects of miR-409-3p on cell proliferation, migration and invasion.

  14. TCLM-P2P: Task Collaboration Logic Model Oriented to P2P Community%TCLM-P2P:面向P2P社区的任务协作逻辑模型

    Institute of Scientific and Technical Information of China (English)

    王杨; 王汝传; 严远亭; 韩志杰; 赵保华

    2012-01-01

    P2P网络中广泛存在的“free riding”现象使其在任务协作领域的应用受到了极大制约.为了实现P2P网络环境下的有效任务协作,提出了一种具有激励机制的任务协作逻辑模型.基于Agent理论,首先给出了对等体、半对等体、P2P社区等概念;然后在合同网的框架下提出了面向P2P网络社区的任务协作逻辑模型TCLM-P2P(task collaborative logic model oriented to P2P community).相对于传统的任务协作模型,在合理的前提假设条件下,模型给出了模型公理和协作规则.该模型通过基于虚拟积分的协作算法实现了具有激励机制的P2P网络中的任务分配与协作.原型系统的实现及仿真实验结果表明TCLM-P2P模型具有可行性和有效性:不仅能够激励自利节点主动参与到任务分配与协作中;同时也能在一定程度上抑制节点的free riding行为,从而保障了P2P系统的有序工作.%Traditional P2P networks mainly are applied to file sharing and instant message fields. However, how to perform the task collaboration based on P2P community is a challenging job. The former research work indicated that the task collaboration in P2P network had been greatly restricted by free riding behaviors. To realize effective task allocating and task collaborating in P2P network environment, this paper presents a task collaboration logic model oriented to P2P community. Based on agent and multi-agent theory, the paper firstly introduces some concepts including the peer body, half-peer body and P2P community; then the TCLM-P2P is presented including some collaboration axioms and rulers. In order to enhance the incentive mechanism, virtual score becomes the main goal which each peer endeavor pursues. In addition, based on the contract net protocol, a task collaboration algorithm is presented. The proposed algorithm is composed of two phases. One is the task collaboration and the other is the task second bid when some peers fail

  15. In vitro phosphorylation and acetylation of the murine pocket protein Rb2/p130.

    Directory of Open Access Journals (Sweden)

    Muhammad Saeed

    Full Text Available The retinoblastoma protein (pRb and the related proteins Rb2/p130 and 107 represent the "pocket protein" family of cell cycle regulators. A key function of these proteins is the cell cycle dependent modulation of E2F-regulated genes. The biological activity of these proteins is controlled by acetylation and phosphorylation in a cell cycle dependent manner. In this study we attempted to investigate the interdependence of acetylation and phosphorylation of Rb2/p130 in vitro. After having identified the acetyltransferase p300 among several acetyltransferases to be associated with Rb2/p130 during S-phase in NIH3T3 cells in vivo, we used this enzyme and the CDK4 protein kinase for in vitro modification of a variety of full length Rb2/p130 and truncated versions with mutations in the acetylatable lysine residues 1079, 128 and 130. Mutation of these residues results in the complete loss of Rb2/p130 acetylation. Replacement of lysines by arginines strongly inhibits phosphorylation of Rb2/p130 by CDK4; the inhibitory effect of replacement by glutamines is less pronounced. Preacetylation of Rb2/p130 strongly enhances CDK4-catalyzed phosphorylation, whereas deacetylation completely abolishes in vitro phosphorylation. In contrast, phosphorylation completely inhibits acetylation of Rb2/p130 by p300. These results suggest a mutual interdependence of modifications in a way that acetylation primes Rb2/p130 for phosphorylation and only dephosphorylated Rb2/p130 can be subject to acetylation. Human papillomavirus 16-E7 protein, which increases acetylation of Rb2/p130 by p300 strongly reduces phosphorylation of this protein by CDK4. This suggests that the balance between phosphorylation and acetylation of Rb2/p130 is essential for its biological function in cell cycle control.

  16. Analysis and Design of Instant Message System Based on P2P

    Institute of Scientific and Technical Information of China (English)

    Shen Zhiwei; Ma Shaowu

    2008-01-01

    By analyzing current development conditions of mainstream Instant Message technologies and services, some detailed discussion about technical solution and formal operation deployment strategies of P2P Instant Message Services based on Interact is made. Several crucial problems of P2P Instant Message System about services management, interconnection with other instant message, security and profit-making mode are analyzed,, and how to develop P2P IM Services is proposed.

  17. IPTV-RM: A Resources Monitoring Architecture for P2P IPTV Systems

    OpenAIRE

    2012-01-01

    Resources monitoring is an important problem of the overall efficient usage and control of P2P IPTV systems. The resources of IPTV can include all distributing servers, programs and peers. Several researches have tried to address this issue, but most of them illuminated P2P traffic characterization, identification and user behavior. The main contributions of this paper are twofold. Firstly, a resources monitoring architecture for P2P IPTV systems, IPTV-RM, was presented based on previous work...

  18. OMAN - A Management Architecture for P2P Service Overlay Networks

    OpenAIRE

    Fiorese, Adriano; Simões, Paulo; Boavida, Fernando

    2010-01-01

    International audience; In a world where networking services are increasingly being provided by service overlay networks, management of these services at overlay level is becoming crucially important. This paper presents an architecture for services management in P2P Service Overlay Networks (SON). The architecture, named OMAN, takes into account the formation of the P2P SON comprising several different service providers belonging to several different network domains. It uses P2P mechanisms t...

  19. Survey of Search and Replication Schemes in Unstructured P2P Networks

    CERN Document Server

    Thampi, Sabu M

    2010-01-01

    P2P computing lifts taxing issues in various areas of computer science. The largely used decentralized unstructured P2P systems are ad hoc in nature and present a number of research challenges. In this paper, we provide a comprehensive theoretical survey of various state-of-the-art search and replication schemes in unstructured P2P networks for file-sharing applications. The classifications of search and replication techniques and their advantages and disadvantages are briefly explained. Finally, the various issues on searching and replication for unstructured P2P networks are discussed.

  20. An Optimized and Improved Network Trust Model Based on P2P

    Directory of Open Access Journals (Sweden)

    Li Wei

    2013-10-01

    Full Text Available Because of the spring up of the P2P network application, its open, anonymous and self-organized characteristics has offered a path for the spreading of virus and junk data, and the security has aroused people’s common concern. The traditional network trust model dealt with dishonest node has certain limitation, for this, improved P2P network trust model and applied D-S evidence theory to the trusted computing of trust model. Through simulation experiment, the result showed: the improved P2P network trust model efficiently increased the successful trading rate of P2P network and improved the network environment

  1. Phagocytes: A Holistic Defense and Protection Against Active P2P Worms

    OpenAIRE

    Chen, Ruichuan; Lua, Eng Keong; Crowcroft, Jon; Tang, Liyong; Chen, Zhong

    2011-01-01

    Active Peer-to-Peer (P2P) worms present serious threats to the global Internet by exploiting popular P2P applications to perform rapid topological self-propagation. Active P2P worms pose more deadly threats than normal scanning worms because they do not exhibit easily detectable anomalies, thus many existing defenses are no longer effective. We propose an immunity system with Phagocytes --- a small subset of elected P2P hosts that are immune with high probability and specialized in finding an...

  2. What Happened to P2P Systems? A Special Focus on Content Distribution Systems

    OpenAIRE

    Mohammad Fawzi, Ali

    2008-01-01

    Peer-to-Peer (P2P) technology has emerged as a new distributed computing paradigm. P2P technology attempts to "harness the powers of the edges of the Internet" by making efficient and effective use of peers (users) at the "edge" of the Internet, by direct interaction between peers of the system. P2P architecture has witnessed lots of interest and research in the latest years because of the popularity of file-sharing applications based on it. Content distribution is an essential P2P applicati...

  3. Research on P2P Overlay Network Model with Small-world Features

    OpenAIRE

    Liu, Hao; Chen, Zhigang

    2013-01-01

    Topology structure of P2P network decides its system performance. However, the existing P2P network models don’t take clustering and symmetry of nodes into account. Based on the algebra and graph theory method of Cayley graph, this paper proposes a novel P2P overlay network model with small-world features. Its simplicity and symmetry can ensure the self-organization and scalability of P2P network. The results of analysis and experiment shows that this model provides better robustness, h...

  4. AFM imaging reveals the assembly of a P2X receptor complex containing P2X2, P2X4 and P2X6 subunits

    OpenAIRE

    2012-01-01

    Seven P2X purinergic receptor subunits have been identified: P2X1-P2X7. All except P2X6 assemble as homotrimers, and six heteromeric receptors (P2X1/2, P2X1/4, P2X1/5, P2X2/3, P2X2/6 and P2X4/6) have been described. In addition, P2X4 homomers associate with P2X2 or P2X7 homomers as dimers of trimers. The various P2X receptors show individual functional properties, suggesting distinct physiological roles. The overlapping expression of P2X2, P2X4 and P2X6 subunits has been shown in different ce...

  5. Institute of Information Network Technology%P2P-SIP融合及P2P-SIP系统设计

    Institute of Scientific and Technical Information of China (English)

    吴亮

    2010-01-01

    随着现代通信技术的不断发展,P2P(对等网络)技术与SIP(会话初始化协议)之间相互融合成为当前研究的一个热点.文章首先介绍了P2P技术和SIP协议,随后研究怎样将P2P架构与SIP系统进行融合,重点讨论了P2P-SIP的实现过程,包括P2P-SIP终端如何使用SIP协议携带P2P消息实现节点的发现、加入、离开和用户注册等过程.

  6. Silane photoabsorption spectra mear the Si 2p thresholds:the geometry of Si 2p excited SiH4

    Institute of Scientific and Technical Information of China (English)

    张卫华; 李家明; 等

    2003-01-01

    Based on the multiple-scattering self-consistent-field method,we have studied the photoabsorption spectra near the Si 2p thresholds of silane,According to our calculations,the clear assignments of the inner-shell photoabsorption spectra are provided.In comparison with the high-resolution experimental spectra,the geometric structure of the Si 2p-excited SiH4 is recommended to be of a C2v symmetry.More specifically,the Si 2p-excited SiH4** have two bond lengths of 2.50 a.u. and another two bond lengths of 2.77 a.u.,and the corresponding two bond angles are 104.0° and 112.5° respectively.

  7. miR-342-3p affects hepatocellular carcinoma cell proliferation via regulating NF-κB pathway

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, Liang; Zhang, Yubao, E-mail: zhyb880077@sina.com

    2015-02-13

    Recent research indicates that non-coding microRNAs (miRNAs) help regulate basic cellular processes in many types of cancer cells. We hypothesized that overexpression of miR-342-3p might affect proliferation of hepatocellular carcinoma (HCC) cells. After confirming overexpression of miR-342-3p with qRT-PCR, MTT assay showed that HCC cell proliferation was significantly inhibited by miR-342-3p, and that it significantly decreased BrdU-positive cell proliferation by nearly sixfold. Searching for targets using three algorithms we found that miR-342-3p is related to the NF-κB pathway and luciferase assay found that IKK-γ, TAB2 and TAB3 are miR-342-3p target genes. Results of western blot on extracted nuclear proteins of HepG2 and HCT-116 cells showed that miR-342-3p reduced and miR-342-3p-in increased p65 nuclear levels and qRT-PCR found that NF-κB pathway downstream genes were downregulated by miR-342-3p and upregulated by miR-342-3p-in, confirming that miR-342 targets NF-κB pathway. Overexpression of Ikk-γ, TAB2 and TAB3 partially rescued HCC cells proliferation inhibited by miR-342-3p. Using the GSE54751 database we evaluated expression from 10 HCC samples, which strongly suggested downregulation of miR-342-3p and we also found inverse expression between miR-342-3p and its targets IKK-γ, TAB2 and TAB3 from 71 HCC samples. Our results show that miR-342-3p has a significant role in HCC cell proliferation and is suitable for investigation of therapeutic targets. - Highlights: • MiR-342-3p suppresses hepatocellular carcinoma cell proliferation. • MiR-342-3p targets IKK-γ, TAB2 and TAB3 genes. • MiR-342-3p downregulates NF-kB signaling pathway. • MiR-342-3p is downregulated in clinical hepatocellular carcinoma samples. • The expression of miR-342-3p and its target gene is inversely related.

  8. Residual Chemosensory Capabilities in Double P2X2/P2X3 Purinergic Receptor Null Mice: Intraoral or Postingestive Detection?

    Science.gov (United States)

    Hallock, Robert M.; Tatangelo, Marco; Barrows, Jennell

    2009-01-01

    Mice lacking the purinergic receptors, P2X2 and P2X3 (P2X2/P2X3Dbl−/−), exhibit essentially no tastant-evoked activity in the chorda tympani and glossopharyngeal nerves and substantial loss of tastant-evoked behavior as measured in long-term intake experiments. To assess whether the residual chemically driven behaviors in these P2X2/P2X3Dbl−/− mice were attributable to postingestive detection or oropharyngeal detection of the compounds, we used brief access lickometer tests to assess the behavioral capabilities of the P2X2/P2X3Dbl−/− animals. The P2X2/P2X3Dbl−/− mice showed avoidance to high levels (10 mM quinine and 10–30 mM denatonium benzoate) of classical “bitter”-tasting stimuli in 24-h, 2-bottle preference tests but minimal avoidance of these substances in the lickometer tests, suggesting that the strong avoidance in the intake tests was largely mediated by post-oral chemosensors. Similarly, increases in consumption of 1 M sucrose by P2X2/P2X3Dbl−/− mice in long-term intake tests were not mirrored by increases in consumption of sucrose in lickometer tests, suggesting that sucrose detection in these mice is mediated by postingestive consequences. In contrast, in brief access tests, P2X2/P2X3Dbl−/− mice avoided citric acid and hydrochloric acid at the same concentrations as their wild-type counterparts, indicating that these weak acids activate oropharyngeal chemoreceptors. PMID:19833662

  9. Fabrication of a TiO2-P25/(TiO2-P25+TiO2 nanotubes) junction for dye sensitized solar cells

    OpenAIRE

    Nguyen Huy Hao; Gobinda Gyawali; Tohru Sekino; Soo Wohn Lee

    2016-01-01

    The dye sensitized solar cell (DSSC), which converts solar light into electric energy, is expected to be a promising renewable energy source for today's world. In this work, dye sensitized solar cells, one containing a single layer and one containing a double layer, were fabricated. In the double layer DSSC structure, the under-layer was TiO2-P25 film, and the top layer consisted of a mixture of TiO2-P25 and TiO2 nanotubes. The results indicated that the efficiency of the DSSC with the double...

  10. MiR-490-3p sensitizes ovarian cancer cells to cisplatin by directly targeting ABCC2

    Science.gov (United States)

    Tian, Jing; Xu, Yan-Ying; Li, Lian; Hao, Quan

    2017-01-01

    Cisplatin (CDDP) resistance becomes a large obstacle of the beneficial therapy for patients with ovarian cancer. MicroRNAs (miRNAs) act as post-transcriptional regulators of multiple genes’ expression and have been reported to be involved in multi-drug resistance. The purpose of this study was to determine the roles and molecular mechanism of miR-490-3p in the CDDP resistance in ovarian cancer. We found that miR-490-3p was downregulated in CDDP-resistant OVCAR3/CDDP and SKOV3/CDDP cells, which was due to the hypermethylation of miR-490-3p promoter. Functional studies demonstrated that miR-490-3p increased the cell response to CDDP in OVCAR3, SKOV3 and CDDP-resistant cells, while miR-490-3p inhibition resulted in opposite effects. Luciferase assay, real-time PCR and Western blot as well as immunohistochemistry validated that ABCC2 was a direct target of miR-490-3p and miR-490-3p downregulated ABCC2 expression via binding to its 3’UTR. Importantly, silencing of ABCC2 alleviated CDDP resistance induced by miR-490-3p inhibition, while ABCC2 overexpression restored CDDP resistance inhibited by miR-490-3p. In vivo study showed that miR-490-3p enhanced the cytotoxicity of CDDP. Finally, we found that miR-490-3p was downregulated in CDDP-resistant ovarian cancer tissues, while ABCC2 was upregulated. In conclusion, our data indicate that miR-490-3p enhances CDDP sensitivity of ovarian cancer cells through downregulating ABCC2 expression, and suggest that delivery of miR-490-3p might be a potential therapeutic strategy for patients with CDP-resistant ovarian cancer.

  11. MicroRNA-127-3p inhibits proliferation and invasion by targeting SETD8 in human osteosarcoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Jun; Hou, Wengen; Chai, Mingxiang; Zhao, Hongxing; Jia, Jinling; Sun, Xiaohui; Zhao, Bin [Second Department of Orthopaedics, The First Affiliated Hospital of XinXiang Medical College, XinXiang 453100 (China); Wang, Ran, E-mail: wangranfire@sohu.com [Department of Pediatrics, The First Affiliated Hospital, ZhengZhou University, ZhengZhou 450000 (China)

    2016-01-22

    MicroRNAs (miRNAs) play an essential role in cancer development. Several studies have indicated that miRNAs mediate tumorigenesis processes, such as, inflammation, proliferation, apoptosis and invasion. In the present study, we focused on the influence of the miR-127-3p on the proliferation, migration and invasion of osteosarcoma (OS). MiR-127-3p was found at reduced levels in OS tissues and cell lines. Overexpression of miR-127-3p in the OS cell lines significantly inhibited the cell proliferation, migration and invasion; however, inhibition of miR-127-3p increased the proliferation, migration and invasion of OS in vitro. SETD8 was identified as a direct target of miR-127-3p, and SETD8 expression decreased post miR-127-3p overexpression, while SETD8 overexpression could reverse the potential influence of miR-127-3p on the migration and invasion of OS cells. MiR-127-3p is suggested to act mainly via the suppression of SETD8 expression. Overall, the results revealed that miR-127-3p acts as a tumor suppressor and that its down-regulation in cancer may contribute to OS progression and metastasis, suggesting that miR-127-3p could be a potential therapeutic target in the treatment of OS. - Highlights: • MiR-127-3p is decreased in osteosarcoma tissues and cell lines. • MiR-127-3p overexpression suppresses cell migration and invasion in MG63 and U2OS. • SETD8 overexpression abolishes the roles of miR-127-3p in osteosarcoma.

  12. Absolute frequency measurement of the 1S0 - 3P0 transition of 171Yb

    CERN Document Server

    Pizzocaro, Marco; Rauf, Benjamin; Bregolin, Filippo; Milani, Gianmaria; Clivati, Cecilia; Costanzo, Giovanni A; Levi, Filippo; Calonico, Davide

    2016-01-01

    We report the absolute frequency measurement of the unperturbed transition 1S0 - 3P0 at 578 nm in 171Yb realized in an optical lattice frequency standard. The absolute frequency is measured 518 295 836 590 863.55(28) Hz relative to a cryogenic caesium fountain with a fractional uncertainty of 5.4x10-16 . This value is in agreement with the ytterbium frequency recommended as a secondary representation of the second in the International System of Units.

  13. Pulsar Spin-Down by 3P2 Superfluid Neutron with Field Decay

    Institute of Scientific and Technical Information of China (English)

    LUO Xin-Lian; PENG Qiu-He; CHOU Chih-Kang

    2003-01-01

    To describe pulsar spin-down, we present a simple combined torque model that takes into account both the standard magnetic dipole radiation and the electromagnetic radiation from the 3P2 superSuid vortex neutrons inside neutron star. Using an ordinary exponential model for the magnetic field decay, we derive an analytical formulae for pulsar evolution tracks. The pulsar evolution on the P-P diagram is quite different from that of the standard magnetic dipole radiation model, especially when the supernuid torque or Geld decay becomes dominant.

  14. Phase transitions in Cd3P2 at high pressures and high temperatures

    DEFF Research Database (Denmark)

    Yel'kin, F.S.; Sidorov, V.A.; Waskowska, A.;

    2008-01-01

    The high-pressure, high-temperature structural behaviour of Cd3P2 has been studied using electrical resistance measurements, differential thermal analysis, thermo baric analysis and X-ray diffraction. At room temperature, a phase transformation is observed at 4.0 GPa in compression....... The experimental zero-pressure bulk modulus of the low-pressure phase is 64.7(7) GPa, which agrees quite well with the calculated value of 66.3 GPa using the tight-binding linear muffin-tin orbital method within the local density approximation. Tentatively, the high-pressure phase has an orthorhombic crystal...

  15. 基于爬行器的大规模P2P IPTV测量%Crawler-Based Measurement of Large Scale P2P IPTV Systems 

    Institute of Scientific and Technical Information of China (English)

    姜志宏; 王晖; 樊鹏翼

    2011-01-01

    为了解大规模P2P IPTV系统中的用户行为特征和拓扑结构特征等内在信息,开发和部署了一个多协议P2P IPTV爬行器TVCrawer,对3个主流的P2P IPTV系统--PPLive,PPStream和UUSee进行了大量的主动测量,并对P2P IPTV系统中的用户行为和网络拓扑特征进行了分析和比较.主要发现包括:1)P2P IPTV系统的频道在线人数中,有一半以上位于不可达的NAT或者防火墙后面;2)节点动态性的波动范围随频道人数的增加而增加,但是其取值范围具有幂律上限;3)节点会话长度符合广延指数分布;4)PPLive的入度分布属于具有指数截断的幂次分布,PPStream的入度表现为某种分段幂律函数,UUSee的入度接近威布尔分布;5)P2P IPTV系统都是异配网络;6)P2P IPTV系统都表现为小世界网络;7)PPLive网络具有聚类特征,而PPStream和UUSee则不存在明显聚类特征;8)3个系统都表现出类似于无标度网络的鲁棒性特征,而与其他两个系统比较时,PPLive具有更高的故障容错性和更明显的攻击脆弱性.这些测量研究和发现不仅有助于设计出更符合真实网络应用环境的系统或协议,也是实现对P2P IPTV进行监测,引导,控制等方面的重要依据和基础.%In order to gain insight on the topology characteristics of P2P IPTV systems and their online user behavior characteristics, This paper develop and deploys a multiprotocol P2P IPTV network crawler, called TVCrawler, which enables users to launch an in-depth measurement and comparative research for several well-known P2P IPTV systems, including PPLive, PPStream, and UUSee. This paper presents results from experiments and research efforts on these large-scale P2P IPTV overlay graphs. Major findings include 1) more than 50% of online users are unreachable because they lie behind NAT or firewall; 2) fluctuation range of churn increases with thc increase of the population of channel, and there exists a power-law upper bound for

  16. Duplicación 5q34q35.3 que involucra el gen NSD1: región delimitada por microarreglos de hibridación genómica comparativa. A propósito de un caso

    Directory of Open Access Journals (Sweden)

    AR Vázquez del Campo

    2016-09-01

    Full Text Available La duplicación de la región 5q34q35.3 se ha asociado a un fenotipo inverso de síndrome de Sotos, por contener el gen NSD1 en doble dosis. Se presenta a una paciente con retraso global del desarrollo, peso y talla bajos y dismorfias. Cuenta con antecedente de corrección quirúrgica por defectos de septación auriculoventricular (CIA y CIV, así como remodelación por antecedente de cráneo en trébol. El cariotipo con bandas G mostró un resultado 46,XX,add(5(q35, y para confirmar el origen del material adicional se utilizó sonda de FISH WCP para el cromosoma 5 [46,XX,add(5(q35.ish dup(5(q35(wcp5+]. Para definir puntos de ruptura y realizar correlación genotipo fenotipo se realizó microarreglo CytoScan HD de Affymetrix, que confirmó la duplicación intersticial de novo de 14Mb 46,XX,add(5(q35. arr[hg19] 5q34q35.3(163,110,984-177,227,216x3,177,259,401- 179,330,764x3,179,346,465 180,719,789x3dn, que contiene 80 genes (USCS genome browser, NCBI36/hg19. La descripción de este caso es importante para complementar la delineación del fenotipo, mediante la correlación con la región de la duplicación a nivel de nucleótidos. Cabe resaltar que la paciente demuestra la importancia y la utilidad de las nuevas técnicas de citogenética molecular, con las cuales es posible el análisis detallado de los genes localizados en la región 5q involucrada, así como su correlación con las manifestaciones clínicas.

  17. Measurement of the isotope shift of the 2{{\\rm{p}}}^{4}{}^{3}{{P}}_{2} \\rightarrow 2{{\\rm{p}}}^{3}3{\\rm{p}}{}^{3}{{P}}_{2} two-photon transition of O I and a revision of the triplet energy levels of atomic oxygen

    Science.gov (United States)

    Marinov, D.; Booth, J.-P.; Drag, C.; Blondel, C.

    2017-03-01

    Two-photon induced fluorescence of atomic oxygen, which is routinely used for plasma and flame diagnostics, is implemented with two counterpropagating laser beams of sufficient monochromaticity to permit Doppler-free spectroscopy. A single-mode injection-seeded pulsed Ti:sapphire laser has been frequency-doubled twice to produce narrow-band pulsed radiation at wavelengths around 225.6 nm, suitable to excite the ground-state-to-2{{{p}}}33{{p}}{}3{P} two-photon resonance line of oxygen. Accurate measurement of the injection-seeding wavelength provides new data on the excitation energy of the 3{{p}}{}3{P} states. The experiment was done both sequentially and simultaneously on 16O and 18O, which makes it possible to test recent calculations of the isotope shift. Having an absolute measurement of the excitation wave-number from the ground level, which has been the case only twice in previous spectroscopic studies of O I, we can re-examine the energy levels of the subset of triplet states and present an updated set of optimised energy values.

  18. 29 CFR 1917.22 - Hazardous cargo 2 (See § 1917.2(p)).

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Hazardous cargo 2 (See § 1917.2(p)). 1917.22 Section 1917.22 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION... § 1917.2(p)). 2 The Department of Transportation and the United States Coast Guard apply...

  19. Data of evolutionary structure change: 1ACMA-2P2GA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1ACMA-2P2GA 1ACM 2P2G A A ANPLYQKHIISINDLSRDDLNLVLATAAKLKANP-QPEL...HHHHH EEE HHHH HHHHHHHHHHHHHHHHHHHH --- 0 1ACM... A 1ACMA ELLKH-KV...256007194519 1 1ACM... A 1ACMA DMLDE----KGI

  20. PD2P : PanDA Dynamic Data Placement for ATLAS

    CERN Document Server

    CERN. Geneva

    2012-01-01

    The PanDA Dynamic Data Placement (PD2P) system has been developed to cope with difficulties of data placement for ATLAS. PD2P is an intelligent subsystem of PanDA to distribute data by taking the following factors into account: popularity, locality, the usage pattern of the data, the distribution of CPU and storage resources, ...

  1. Data of evolutionary structure change: 1BFGA-2P23A [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1BFGA-2P23A 1BFG 2P23 A A ---DPKRLYC-KNGG---FFLRIHPDGRVDGVREKSDPH.../confEVID> 0 1BFG A 1BFG...dex> 1BFG A 1BFGA GS...4 HIS CA 324 SER CA 410 LEU CA 389 1BFG... A 1BFGA GVREKSDPHIK

  2. Pseudogene CYP4Z2P 3′UTR promotes angiogenesis in breast cancer

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, Lufeng; Li, Xiaoman; Gu, Yi; Ma, Yihua; Xi, Tao, E-mail: xitao18@hotmail.com

    2014-10-24

    Highlights: • A new critical role of pseudogene CYP4Z2P 3′UTR in breast cancer is proposed. • We examine the level of pseudogene CYP4Z2P 3′UTR in breast cancer tissues. • The functions of CYP4Z2P 3′UTR and mechanism were studied. • The mechanism provides new insights for the breast cancer progression. - Abstract: Pseudogenes have long been marked as “false” genes, which are similar with real genes but have no apparent function. The 3′UTR is well-known to regulate gene expression post-transcriptionally. Our recent evidence, however, indicates novel functional roles of pseudogene CYP4Z2P 3′UTR (Z2P-UTR). We found that ectopic expression of Z2P-UTR in breast cancer cells significantly increased the expression of VEGF-A without affecting cell proliferation in vitro. Meanwhile, conditioned medium (CM) from Z2P-UTR overexpression cells enhanced proliferation, migration and tube formation of HUVEC, and promoted angiogenesis in ex vivo models. Also, CM increased the expression of VEGFR2 in HUVEC. Our data suggest that Z2P-UTR can promote breast cancer angiogenesis partly via paracrine pathway of VEGF-A/VEGFR2.

  3. Phagocytes: A Holistic Defense and Protection Against Active P2P Worms

    CERN Document Server

    Chen, Ruichuan; Crowcroft, Jon; Tang, Liyong; Chen, Zhong

    2011-01-01

    Active Peer-to-Peer (P2P) worms present serious threats to the global Internet by exploiting popular P2P applications to perform rapid topological self-propagation. Active P2P worms pose more deadly threats than normal scanning worms because they do not exhibit easily detectable anomalies, thus many existing defenses are no longer effective. We propose an immunity system with Phagocytes --- a small subset of elected P2P hosts that are immune with high probability and specialized in finding and "eating" worms in the P2P overlay. The Phagocytes will monitor their managed P2P hosts' connection patterns and traffic volume in an attempt to detect active P2P worm attacks. Once detected, local isolation, alert propagation and software patching will take place for containment. The Phagocytes further provide the access control and filtering mechanisms for communication establishment between the internal P2P overlay and the external hosts. We design a novel adaptive and interaction-based computational puzzle scheme at ...

  4. The iron L edges : Fe 2p X-ray absorption and electron energy loss spectroscopy

    NARCIS (Netherlands)

    Miedema, Piter S.; de Groot, Frank M. F.

    The iron L edges are reviewed. The experimental spectra obtained with 2p XAS, XMCD and 2p EELS are discussed, including isolated iron atoms, solids and coordination compounds. The largest fraction of publications deal with iron oxide systems that are discussed in detail, including binary oxides,

  5. 2p3d Resonant X-ray emission spectroscopy of cobalt compounds

    NARCIS (Netherlands)

    van Schooneveld, M.M.

    2013-01-01

    This manuscript demonstrates that 2p3d resonant X-ray emission spectroscopy (RXES) yields unique information on the chemically relevant valence electrons of transition metal atoms or ions. Experimental data on cobalt compounds and several theories were used hand-in-hand. In chapter 1 2p3d RXES was s

  6. Relative brightness of the O{sup +}({sup 2} D-{sup 2} P) doublets in low-energy aurorae

    Energy Technology Data Exchange (ETDEWEB)

    Whiter, D. K. [Finnish Meteorological Institute, Erik Palménin Aukio 1, FI-00560 Helsinki (Finland); Lanchester, B. S.; Gustavsson, B.; Jallo, N. I. B.; Jokiaho, O.; Dahlgren, H. [School of Physics and Astronomy, University of Southampton, SO17 1BJ (United Kingdom); Ivchenko, N., E-mail: daniel.whiter@fmi.fi [Royal Institute of Technology (KTH), Teknikringen 31, SE-100 44 Stockholm (Sweden)

    2014-12-10

    The ratio of the emission line doublets from O{sup +} at 732.0 nm (I {sub 732}) and 733.0 nm (I {sub 733}) has been measured in auroral conditions of low-energy electron precipitation from Svalbard (78.°20 north, 15.°83 east). Accurate determination of R = I {sub 732}/I {sub 733} provides a powerful method for separating the density of the O{sup +} {sup 2} P{sub 1} {sub /2,3} {sub /2}{sup o} levels in modeling of the emissions from the doublets. A total of 383 spectra were included from the winter of 2003-2004. The value obtained is R = I {sub 732}/I {sub 733} = 1.38 ± 0.02, which is higher than theoretical values for thermal equilibrium in fully ionized plasma, but is lower than reported measurements by other authors in similar auroral conditions. The continuity equations for the densities of the two levels are solved for different conditions, in order to estimate the possible variations of R. The results suggest that the production of ions in the two levels from O ({sup 3} P {sub 1}) and O ({sup 3} P {sub 2}) does not follow the statistical weights, unlike astrophysical calculations for plasmas in nebulae. The physics of auroral impact ionization may account for this difference, and therefore for the raised value of R. In addition, the auroral solution of the densities of the ions, and thus of the value of R, is sensitive to the temperature of the neutral atmosphere. Although the present work is a statistical study, it shows that it is necessary to determine whether there are significant variations in the ratio resulting from non-equilibrium conditions, from auroral energy deposition, large electric fields, and changes in temperature and composition.

  7. The reactions of O({sup 3}P) with alkenes: The formyl radical channel

    Energy Technology Data Exchange (ETDEWEB)

    Min, Z.; Wong, T.H.; Quandt, R.; Bersohn, R.

    1999-12-09

    The HCO product of the reaction of O({sup 3}P) with ethene has been detected by cavity ring-down spectroscopy using its A-X transition. For propene a somewhat smaller yield of HCO was obtained but the overall rate constant is much larger. The yield of HCO in this reaction is quite small ({approximately}0.05). Moreover, a large number of other alkenes were tried with negative results. The failure of the 1,2 H atom shift followed by breaking the 1,2 bond implies that the unimolecular decomposition has found a more favorable channel. The proposed mechanism is as follows. For an alkene of the form RCH{sub 2}{double{underscore}bond}CH{sub 2} the first step is attachment of the O({sup 3}P) to the terminal carbon atom, C{sub 1}. Then, intersystem crossing occurs and finally a H atom shifts from C{sub 3} to C{sub 2} and not from C{sub 1} to C{sub 2}. In this way a molecule of formaldehyde and an alkene shorter by one carbon atom are formed.

  8. Metalloid tolerance based on phytochelatins is not functionally equivalent to the arsenite transporter Acr3p.

    Science.gov (United States)

    Wysocki, Robert; Clemens, Stephan; Augustyniak, Daria; Golik, Pawel; Maciaszczyk, Ewa; Tamás, Markus J; Dziadkowiec, Dorota

    2003-05-01

    Active transport of metalloids by Acr3p and Ycf1p in Saccharomyces cerevisiae and chelation by phytochelatins in Schizosaccharomyces pombe, nematodes, and plants represent distinct strategies of metalloid detoxification. In this report, we present results of functional comparison of both resistance mechanisms. The S. pombe and wheat phytochelatin synthase (PCS) genes, when expressed in S. cerevisiae, mediate only modest resistance to arsenite and thus cannot functionally compensate for Acr3p. On the other hand, we show for the first time that phytochelatins also contribute to antimony tolerance as PCS fully complement antimonite sensitivity of ycf1Delta mutant. Remarkably, heterologous expression of PCS sensitizes S. cerevisiae to arsenate, while ACR3 confers much higher arsenic resistance in pcsDelta than in wild-type S. pombe. The analysis of PCS and ACR3 homologues distribution in various organisms and our experimental data suggest that separation of ACR3 and PCS genes may lead to the optimal tolerance status of the cell.

  9. SPF-A*: Searching Multimedia Data in Heterogeneous Mobile P2P Network

    Directory of Open Access Journals (Sweden)

    Fan Ye

    2009-04-01

    Full Text Available Mobile P2P (MP2P network has been widely used in everyday life combined with the wireless technology development. However, most previous works concentrate on the assumption that the mobile peers are identical of their inner properties in compositing a MP2P network. However, in current network environment, heterogeneous devices are widely used; they may have different storage sizes, data transmission rate, processing ability and even security levels. In such kind of MP2P Network environment, how to make data transmission effectively is challenge. In this paper, we concentrate on how to effectively obtain a queried multimedia resource and generate a path for such data transmission on the overlay MP2P network, so as to make the resource more effectively transmit among the heterogeneous peers. A new path generating algorithm called SPF-A* (Super Peer First A* is devised, and simulation studies are conducted to validate our proposal.

  10. A Trust Scheme Based DRM Model for P2P System

    Institute of Scientific and Technical Information of China (English)

    XIAO Shangqin; LU Zhengding; LING Hefei; ZOU Fuhao

    2006-01-01

    With the maturation of P2P technology, there are more and more challenges to the protection of digital rights.DRM for traditional Client/Server model can not satisfy the requirement of digital right protection of P2P network.With practical application of P2P network technology and new generational DRM technology, the article proposes a DRM model for P2P system based on trust degree.The proposed model shares the secret key to the trusty peers and affords necessary security ensure for digital content delivery based on P2P technology.Mathematic analyses and simulations show that, compared to the current DRM model, the proposed model is more robust toleration of transmit and security.

  11. Fuzzy-rule-based Adaptive Resource Control for Information Sharing in P2P Networks

    Science.gov (United States)

    Wu, Zhengping; Wu, Hao

    With more and more peer-to-peer (P2P) technologies available for online collaboration and information sharing, people can launch more and more collaborative work in online social networks with friends, colleagues, and even strangers. Without face-to-face interactions, the question of who can be trusted and then share information with becomes a big concern of a user in these online social networks. This paper introduces an adaptive control service using fuzzy logic in preference definition for P2P information sharing control, and designs a novel decision-making mechanism using formal fuzzy rules and reasoning mechanisms adjusting P2P information sharing status following individual users' preferences. Applications of this adaptive control service into different information sharing environments show that this service can provide a convenient and accurate P2P information sharing control for individual users in P2P networks.

  12. Solid state synthesis and characterization of iron(Ⅱ)pyrophosphate Fe2P2O7

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Offwhite pure Fe2P2O7 was synthesized through solid phase reaction using Fe2O3 and NH4H2PO4 in argon atmosphere. The reaction products of Fe2O3 and NH4H2PO4 at a series of temperatures from 400 to 900 ℃ were characterized by XRD. Comparison and analysis of XRD patterns of resultant products indicated well-crystallized Fe2P2O7 could be obtained over 630 ℃ and Fe2P2O7 prepared at 700 ℃ was triclinic in cell type. Comparison of the cell parameters proved that the as-prepared Fe2P2O7 belonged to β-Fe2P2O7 in crystal phase and SEM showed its size distribution was 0.5-2 μm.

  13. A Free-Rider Forecasting Model Based on Gray System Theory in P2P Networks

    Directory of Open Access Journals (Sweden)

    He Xu

    2012-11-01

    Full Text Available The aim of this study is to forecast the number of free-riders in P2P networks which can help network managers to know the status of the networks in advance and take appropriate measures to cope with free-riding behavior. Free-riding behavior is common in P2P networks, which has a negative impact on the robustness, availability and stability of the networks. Severe free-riding behavior may lead to the crash of the whole P2P application system. Based on the research of free-riding behavior in P2P networks, this paper constructs a free-rider forecasting model (GST model using Gray System Theory. Simulation experiments show that this model has high feasibility, and can carry out reasonable predictions on the number of free-riders in P2P networks.

  14. Computing and Routing for Trust in Structured P2P Network

    Directory of Open Access Journals (Sweden)

    Biao Cai

    2009-09-01

    Full Text Available Study of trust in P2P network now is focus on how to effectively against various malicious behaviors such as providing fake or misleading feedback about other peers and the management of trust in a P2P environment. But the scotoma of portability that trust peer can join (leave a certain P2P network at anytime and anywhere is seldom discussed. In this paper, a structured topology for trusts management in portable P2P network based on DHT (discrete hash table is proposed first, in which includes trust management strategies and peer operations on certain DHT circle. After that, a novel trust-computing model for the structured P2P network and the main trust decisions in the structured network are introduced too. Effectiveness and practicality of the proposed trust management have been showed in simulation experiments at the end.

  15. Convergence of Internet and TV: The Commercial Viability of P2P Content Delivery

    Science.gov (United States)

    de Boever, Jorn

    The popularity of (illegal) P2P (peer-to-peer) file sharing has a disruptive impact on Internet traffic and business models of content providers. In addition, several studies have found an increasing demand for bandwidth consuming content, such as video, on the Internet. Although P2P systems have been put forward as a scalable and inexpensive model to deliver such content, there has been relatively little economic analysis of the potentials and obstacles of P2P systems as a legal and commercial content distribution model. Many content providers encounter uncertainties regarding the adoption or rejection of P2P networks to spread content over the Internet. The recent launch of several commercial, legal P2P content distribution platforms increases the importance of an integrated analysis of the Strengths, Weaknesses, Opportunities and Threats (SWOT).

  16. Research on P2P Overlay Network Model with Small-world Features

    Directory of Open Access Journals (Sweden)

    Hao LIU

    2013-09-01

    Full Text Available Topology structure of P2P network decides its system performance. However, the existing P2P network models don’t take clustering and symmetry of nodes into account. Based on the algebra and graph theory method of Cayley graph, this paper proposes a novel P2P overlay network model with small-world features. Its simplicity and symmetry can ensure the self-organization and scalability of P2P network. The results of analysis and experiment shows that this model provides better robustness, higher enquiry efficiency and better load balance than the existing P2P Overlay Network models such as Chord and CAN. Furthermore, it possesses the property of high clustering.

  17. Fabrication of a TiO2-P25/(TiO2-P25+TiO2 nanotubes) junction for dye sensitized solar cells

    Institute of Scientific and Technical Information of China (English)

    Nguyen Huy Hao; Gobinda Gyawali; Tohru Sekino; Soo Wohn Lee

    2016-01-01

    The dye sensitized solar cell (DSSC), which converts solar light into electric energy, is expected to be a promising renewable energy source for today's world. In this work, dye sensitized solar cells, one con-taining a single layer and one containing a double layer, were fabricated. In the double layer DSSC structure, the under-layer was TiO2-P25 film, and the top layer consisted of a mixture of TiO2-P25 and TiO2 nanotubes. The results indicated that the efficiency of the DSSC with the double layer structure was a significant improvement in comparison to the DSSC consisting of only a single film layer. The addition of TiO2-P25 in the top layer caused an improvement in the adsorption of dye molecules on the film rather than on the TiO2 nanotubes only. The presence of the TiO2 nanotubes together with TiO2-P25 in the top layer revealed the enhancement in harvesting the incident light and an improvement of electron transport through the film.

  18. Fabrication of a TiO2-P25/(TiO2-P25+TiO2 nanotubes junction for dye sensitized solar cells

    Directory of Open Access Journals (Sweden)

    Nguyen Huy Hao

    2016-08-01

    Full Text Available The dye sensitized solar cell (DSSC, which converts solar light into electric energy, is expected to be a promising renewable energy source for today's world. In this work, dye sensitized solar cells, one containing a single layer and one containing a double layer, were fabricated. In the double layer DSSC structure, the under-layer was TiO2-P25 film, and the top layer consisted of a mixture of TiO2-P25 and TiO2 nanotubes. The results indicated that the efficiency of the DSSC with the double layer structure was a significant improvement in comparison to the DSSC consisting of only a single film layer. The addition of TiO2-P25 in the top layer caused an improvement in the adsorption of dye molecules on the film rather than on the TiO2 nanotubes only. The presence of the TiO2 nanotubes together with TiO2-P25 in the top layer revealed the enhancement in harvesting the incident light and an improvement of electron transport through the film.

  19. Chromosomal localization of the parathyroid hormone/parathyroid hormone-related protein receptor gene to human chromosome 3p21.1-p24.2

    Energy Technology Data Exchange (ETDEWEB)

    Gelbert, L.; Heath, H. III [Univ. of Utah Health Sciences Center, Salt Lake City, UT (United States); Schipani, E. [Massachusetts General Hospital, Boston, MA (United States)] [and others

    1994-10-01

    The human PTH/PTH-related peptide (PTH/PTHrP) receptor could be involved in hereditary disorders of PTH or PTHrP action. Knowledge of the gene`s chromosomal location would allow studies linking it to specific disease traits. Therefore, we mapped the human PTH/PTHrP receptor gene by polymerase chain reaction of human/rodent somatic cell hybrid panels using oligonucleotide primers designed to amplify a portion of the gene from genomic DNA. The PTH/PTHrP gene was unambiguously assigned to the short arm of human chromosome 3, in the region designated 3p21.1-p24.2. Analysis of a second chromosome 3-specific mapping panel suggests that the gene is located near the 3p21.2-p21.3 boundary. The availability of highly polymorphic markers located in this region will permit exploration of the PTH/PTHrP receptor locus in genetic linkage searches for the causes of bone, calcium, and other potential disorders. 14 refs., 2 figs.

  20. Immunoexpression of the COX-2, p53, and caspase-3 proteins in colorectal adenoma and non-neoplastic mucosa

    Science.gov (United States)

    Nogueira, Renan Brito; Pires, Andréa Rodrigues Cordovil; Soares, Thélia Maria Santos; Rodrigues, Simone Rabello de Souza; Campos, Mariane Antonieta Menino; Toloi, Giovanna Canato; Waisberg, Jaques

    2013-01-01

    ABSTRACT Objective: To analyze the immunoexpression of the COX-2, p53, and caspase-3 proteins in colorectal adenomas and non-neoplastic mucosa. Methods: 72 individuals were subjected to colonoscopy, which provided 50 samples of adenomas and 45 samples of non-neoplastic colorectal mucosa. The tissue samples were obtained via the tissue microarray technique and subjected to immunohistochemical analysis using primary anti-p53, anti-COX-2, and anti-caspase-3 antibodies. The positivity and intensity of the immunoreaction were classified. The analyzed variables were as follows: site of the adenomas in the colon, degree of dysplasia, size, and score of positivity and intensity of immunoexpression of the p-53, caspase-3, and COX-2 proteins. Results: The immunoexpression of mutated protein p53 was positive in 30 (60%) adenoma samples and negative in 20 (40%) adenoma samples. The immunoexpression of mutated protein p53 was negative in 39 (86.6%) samples and positive in 6 (13.3%) samples of the non-neoplastic colorectal mucosa (p<0.0001). Significant differences were seen between both the largest size (p=0.006) and the highest degree of dysplasia (p<0.0001) of the adenomas and the intensity of immunoexpression of mutated protein p53. The positivity and intensity of immunoexpression of COX-2 (p=0.14) and caspase-3 (p=0.23) showed no significant differences between the adenomas and the non-neoplastic colorectal mucosa. Conclusion: Mutated protein p53 was hyperexpressed in the adenomas compared with the non-neoplastic mucosa. Greater size and greater degree of dysplasia in the adenomas were associated with higher expression of mutated protein p53. The immunoexpression of COX-2 and caspase-3 in the adenomas did not exhibit a correlation with the anatomical-pathological features of the tumors and did not differ from the corresponding expression levels in the non-neoplastic mucosa. PMID:24488384

  1. MicroRNA-409-3p inhibits osteosarcoma cell migration and invasion by targeting catenin-δ1.

    Science.gov (United States)

    Wu, Shifeng; Du, Xinjie; Wu, Manwu; Du, Hechun; Shi, Xiaoliang; Zhang, Tao

    2016-06-10

    Osteosarcoma is the most common primary bone cancer which is associated with early metastatic potential and poor prognosis. However, the molecular mechanisms underlying osteosarcoma progression are not well characterized. Here, we investigated the role of miR-409-3p in osteosarcoma metastasis. Osteosarcoma tissue showed decreased expression of miR-409-3p compared to adjacent non-tumorous tissue. The expression level of miR-409-3p was negatively correlated with osteosarcoma metastasis. Overexpression of miR-409-3p in osteosarcoma cells (U2OS) inhibited cell migration and invasion. Bioinformatics analysis showed that catenin-δ1 (CTNND1, p120-catenin) is a direct target of miR-409-3p. Overexpression of miR-409-3p repressed the expression of catenin-δ1 in U2OS cells at both mRNA and protein levels. Meanwhile, miR-409-3p repressed the activity of luciferase reporter containing the 3'-untranslated region (3'UTR) of CTNND1 gene. Furthermore, expression of catenin-δ1 rescued the inhibitory effect of miR-409-3p on cell migration and invasion. Altogether, these results indicated that miR-409-3p targets catenin-δ1 to repress osteosarcoma metastasis.

  2. Laser Flash Photolysis Studies of Radical-Radical Reaction Kinetics: The O((sup 3)P(sub J)) + BrO Reaction

    Science.gov (United States)

    Thorn, R. P.; Cronkhite, J. M.; Nicovich, J. M.; Wine, P. H.

    1997-01-01

    A novel dual laser flash photolysis-long path absorption-resonance fluorescence technique has been employed to study the kinetics of the important stratospheric reaction 0((sup 3)P(sub j)) + Br yields(k1) BrO((sup 2)P(sub J)) + O2 as a function of temperature (231-328 K) and pressure (25-150 Torr) in N2 buffer gas. The experimental approach preserves the principal advantages of the flash photolysis method, i.e., complete absence of surface reactions and a wide range of accessible pressures, but also employs techniques which are characteristic of the discharge flow method, namely chemical titration as a means for deducing the absolute concentration of a radical reactant and use of multiple detection axes. We find that k1 is independent of pressure, and that the temperature dependence of k1 is adequately described by the Arrhenius expression k1(T) = 1.91 x 10(exp -11)(230/J) cu cm/ molecule.s; the absolute accuracy of measured values for k1 is estimated to vary from +/- 20 percent at at T approximately 230 K to +/- 30 percent at T approximately 330 K. Our results demonstrate that the O((sup 3)P(sub j)) + BrO rate coefficient is significantly faster than previously 'guesstimated,' and suggest that the catalytic cycle with the O((sup 3)P(sub j)) + BrO reaction as its rate-limiting step is the dominant stratospheric BrO(x), odd-oxygen destruction cycle at altitudes above 24 km.

  3. Automated Simulation P2P Botnets Signature Detection by Rule-based Approach

    Directory of Open Access Journals (Sweden)

    Raihana Syahirah Abdullah

    2016-08-01

    Full Text Available Internet is a most salient services in communication. Thus, companies take this opportunity by putting critical resources online for effective business organization. This has given rise to activities of cyber criminals actuated by botnets. P2P networks had gained popularity through distributed applications such as file-sharing, web caching and network storage whereby it is not easy to guarantee that the file exchanged not the malicious in non-centralized authority of P2P networks. For this reason, these networks become the suitable venue for malicious software to spread. It is straightforward for attackers to target the vulnerable hosts in existing P2P networks as bot candidates and build their zombie army. They can be used to compromise a host and make it become a P2P bot. In order to detect these botnets, a complete flow analysis is necessary. In this paper, we proposed an automated P2P botnets through rule-based detection approach which currently focuses on P2P signature illumination. We consider both of synchronisation within a botnets and the malicious behaviour each bot exhibits at the host or network level to recognize the signature and activities in P2P botnets traffic. The rule-based approach have high detection accuracy and low false positive.

  4. Functional Diversification of Fungal Glutathione Transferases from the Ure2p Class

    Directory of Open Access Journals (Sweden)

    Anne Thuillier

    2011-01-01

    Full Text Available The glutathione-S-transferase (GST proteins represent an extended family involved in detoxification processes. They are divided into various classes with high diversity in various organisms. The Ure2p class is especially expanded in saprophytic fungi compared to other fungi. This class is subdivided into two subclasses named Ure2pA and Ure2pB, which have rapidly diversified among fungal phyla. We have focused our analysis on Basidiomycetes and used Phanerochaete chrysosporium as a model to correlate the sequence diversity with the functional diversity of these glutathione transferases. The results show that among the nine isoforms found in P. chrysosporium, two belonging to Ure2pA subclass are exclusively expressed at the transcriptional level in presence of polycyclic aromatic compounds. Moreover, we have highlighted differential catalytic activities and substrate specificities between Ure2pA and Ure2pB isoforms. This diversity of sequence and function suggests that fungal Ure2p sequences have evolved rapidly in response to environmental constraints.

  5. Functional diversification of fungal glutathione transferases from the ure2p class.

    Science.gov (United States)

    Thuillier, Anne; Ngadin, Andrew A; Thion, Cécile; Billard, Patrick; Jacquot, Jean-Pierre; Gelhaye, Eric; Morel, Mélanie

    2011-01-01

    The glutathione-S-transferase (GST) proteins represent an extended family involved in detoxification processes. They are divided into various classes with high diversity in various organisms. The Ure2p class is especially expanded in saprophytic fungi compared to other fungi. This class is subdivided into two subclasses named Ure2pA and Ure2pB, which have rapidly diversified among fungal phyla. We have focused our analysis on Basidiomycetes and used Phanerochaete chrysosporium as a model to correlate the sequence diversity with the functional diversity of these glutathione transferases. The results show that among the nine isoforms found in P. chrysosporium, two belonging to Ure2pA subclass are exclusively expressed at the transcriptional level in presence of polycyclic aromatic compounds. Moreover, we have highlighted differential catalytic activities and substrate specificities between Ure2pA and Ure2pB isoforms. This diversity of sequence and function suggests that fungal Ure2p sequences have evolved rapidly in response to environmental constraints.

  6. Bandwidth Reduction via Localized Peer-to-Peer (P2P Video

    Directory of Open Access Journals (Sweden)

    Ken Kerpez

    2010-01-01

    Full Text Available This paper presents recent research into P2P distribution of video that can be highly localized, preferably sharing content among users on the same access network and Central Office (CO. Models of video demand and localized P2P serving areas are presented. Detailed simulations of passive optical networks (PON are run, and these generate statistics of P2P video localization. Next-Generation PON (NG-PON is shown to fully enable P2P video localization, but the lower rates of Gigabit-PON (GPON restrict performance. Results here show that nearly all of the traffic volume of unicast video could be delivered via localized P2P. Strong growth in video delivery via localized P2P could lower overall future aggregation and core network bandwidth of IP video traffic by 58.2%, and total consumer Internet traffic by 43.5%. This assumes aggressive adoption of technologies and business practices that enable highly localized P2P video.

  7. MiR-519d-3p suppresses invasion and migration of trophoblast cells via targeting MMP-2.

    Directory of Open Access Journals (Sweden)

    Jie Ding

    Full Text Available Our study was approved by the Medical Ethics Committee of Tang Du Hospital, Fourth Military Medical University and complied strictly with national ethical guidelines. Preeclampsia (PE is a specific clinical disorder characterized by gestational hypertension and proteinuria and is a leading cause of maternal and perinatal mortality worldwide. The miR-519d-3p is upregulated in the maternal plasma of patients with PE which indicates a possible association between this microRNA and the pathogenesis of PE. No studies to date have addressed the effect of miR-519d-3p on the invasion and migration of trophoblast cells. In our study, we found that miR-519d-3p expression was elevated in placental samples from patients with PE. In vitro, overexpression of miR-519d-3p significantly inhibited trophoblast cell migration and invasion, whereas transfection of a miR-519d-3p inhibitor enhanced trophoblast cell migration and invasion. Luciferase assays confirmed that matrix metalloproteinase-2 (MMP-2 is a direct target of miR-519d-3p. Quantitative real-time PCR and western blot assays showed that overexpression of miR-519d-3p downregulated MMP-2 mRNA and protein expression. Knockdown of MMP-2 using a siRNA attenuated the increased trophoblast migration and invasion promoted by the miR-519d-3p inhibitor. In placentas from patients with PE or normal pregnancies, a negative correlation between the expression of MMP-2 and miR-519d-3p was observed using the Pearson correlation and linear regression analysis. Our present findings suggest that upregulation of miR-519d-3p may contribute to the development of PE by inhibiting trophoblast cell migration and invasion via targeting MMP-2; miR-519d-3p may represent a potential predictive and therapeutic target for PE.

  8. MicroRNA-27a-3p Inhibits Melanogenesis in Mouse Skin Melanocytes by Targeting Wnt3a.

    Science.gov (United States)

    Zhao, Yuanyuan; Wang, Pengchao; Meng, Jinzhu; Ji, Yuankai; Xu, Dongmei; Chen, Tianzhi; Fan, Ruiwen; Yu, Xiuju; Yao, Jianbo; Dong, Changsheng

    2015-05-14

    MicroRNAs (miRNAs) play an essential role in the regulation of almost all the biological processes, including melanogenesis. MiR-27a-3p is nearly six times higher in white alpaca skin compared to brown skin, which indicates that miR-27a-3p may be a candidate regulator for melanogenesis. Wnt3a plays an important role in promoting melanoblasts to differentiate into melanocytes and melanogenesis. To confirm the function of miR-27a-3p to melanogenesis in mammals, miR-27a-3p mimic, inhibitor and their negative control were transfected into mouse melanocytes. As a result, miR-27a-3p inhibits melanogenesis by repressing Wnt3a at post-transcriptional level. A significant decrease in Wnt3a luciferase activity was observed in 293T cells co-transfected with the matched luciferase reporter vector and pre-miR-27a. Furthermore, the presence of exogenous miR-27a-3p significantly decreased Wnt3a protein expression rather than mRNA and reduced β-catenin mRNA levels in melanocytes. The over-expression of miR-27a-3p significantly increased the melanin content of melanocytes. However, miR-27a-3p inhibitor performs an opposite effect on melanogenesis. Wnt3a is one target of miR-27a-3p. MiR-27a-3p could inhibit Wnt3a protein amount by post-transcriptional regulation and melanogenesis in mouse melanocytes. Previous studies reported that Wnt3a promoted melanogenensis in mouse melanocytes. Thus, miR-27-3p inhibits melanogenesis by repressing Wnt3a protein expression.

  9. P2 P 网络借贷的运营与法律监管%Regulation on Online P2P Lending

    Institute of Scientific and Technical Information of China (English)

    樊云慧

    2014-01-01

    The rapid development of online person -to -person lending poses great challenge to its regula-tion.In the United States ,the current regulatory structure for online P 2 P lending involves multiple overseeing agen-cies including the United States Securities and Exchange Commission , various federal bank regulators , the Consum-er Financial Protection Bureau and the state counterparts of all these entities .Under this system , the protection on lenders mainly depends on the securities regulation .From April 2014, P2P lending came under the regulatory man-date of the Financial Conduct Authority.Our country hasn’t started regulation on online P2P lending.Due to the public interests it involves , the necessity for online P2P lending to prevent risk and develop healthily requires its regulation as soon as possible .The sphere of online P2P lending is similar to financial business , so it should be regulated by financial regulatory agency .Under the current financial regulation system in China ,banking regulatory commission should be the authority to regulate on online P 2P lending.%P2P网络借贷的迅猛发展给监管带来了巨大的挑战。在美国,P2P网络借贷实行由证券监管部门、银行监管部门、金融消费者保护局等多个部门共同监管的分散监管体制,对投资者的保护主要是通过证券监管实现的。英国自2014年4月起将P2P网络借贷纳入金融行为局( FCA)的监管范畴。我国尚未对P2P网络借贷予以监管,但P2P行业因涉及公众利益,出于风险防范和其健康发展的需要,监管问题必须尽快予以解决。由于P2 P借贷公司从事的是类金融业务,故应由监管金融业务的机构予以监管。在我国现行的金融监管体制下,对P2 P网络借贷实行统一监管的机构应为银监会。

  10. Mechanisms and Kinetics of Radical Reaction of O(1D,3P) + HCN System

    Institute of Scientific and Technical Information of China (English)

    HUANG Yu-Cheng; DU Jin-Yan; JU Xue-Hai; YE Shi-Yong; ZHOU Tao

    2008-01-01

    The reaction of HCN with O(1D, 3P) radical has been investigated by density functional theory (DFT) and ab initio methods. The stationary points on the reaction paths(reactants, intermediates and products) were optimized at the (U)B3LYP/aug-cc-pVTZ level.Single-point calculations were performed at the (U)QCISD(T)/aug-cc-pVTZ level for the optimized structures and all the total energies were corrected by zero-point energy. It is shown that there exist three competing mechanisms of oxygen attacking nitrogen O→N, oxygen attacking carbon O→C and oxygen attacking hydrogen O→H. The rate constants were obtained via Eyring transition-state theory in the temperature range of 600~2000 K. The linear relationship between lnk and 1/T was presented. The results show that path 1 is the main reaction channel and the product of NCO + H is predominant.

  11. Narrow-linewidth cooling of $^{6}$Li atoms using the 2S-3P transition

    CERN Document Server

    Chen, Hao-Ze; Wu, Yu-Ping; Liu, Xiang-Pei; Wang, Xiao-Qiong; Chen, Yu-Ao; Pan, Jian-Wei

    2016-01-01

    We report on a narrow-linewidth cooling of $^{6}$Li atoms using the $2S_{1/2}\\to 3P_{3/2}$ transition in the ultraviolet (UV) wavelength regime. By combining the traditional red magneto-optical trap (MOT) at 671 nm and the UV MOT at 323 nm, we obtain a cold sample of $1.3\\times10^9$ atoms with a temperature of 58 $\\mu$K. Furthermore, we demonstrate a high efficiency magnetic transport for $^{6}$Li atoms with the help of the UV MOT. Finally, we obtain $8.1\\times10^8$ atoms with a temperature of 296 $\\mu$K at a magnetic gradient of 198 G/cm in the science chamber with a good vacuum environment and large optical access.

  12. C-H activation of ethers by pyridine tethered PCsp3P-type iridium complexes.

    Science.gov (United States)

    Cui, Peng; Babbini, Dominic C; Iluc, Vlad M

    2016-06-14

    Iridium PCsp3P complexes featuring a novel bis(2-diphenylphosphinophenyl)-2-pyridylmethane ligand (PC(Py)HP) are reported. C-H activation reactions between the dihydride complex [(PC(Py)P)Ir(H)2] and tetrahydrofuran or methyl tert-butyl ether in the presence of a hydrogen acceptor, norbornene (NBE), at ambient temperature led exclusively to the hydrido oxyalkyl complexes, [(PC(Py)P)IrH(C4H7O)] and [(PC(Py)P)IrH(CH2O(t)Bu)], respectively. The internal pyridine donor is important and stabilizes these species by coordination to the iridium center. The coordination of pyridine to the iridium center is labile, however, and its dissociation occurs in the presence of a suitable substrate, as demonstrated by the intramolecular nucleophilic attack of pyridine on a vinylidene intermediate generated from PhC[triple bond, length as m-dash]CH.

  13. Product lambda-doublet ratios for the O(3P) + D2 reaction: A mechanistic imprint

    CERN Document Server

    Jambrina, P G; Aldegunde, J; Brouard, M; Aoiz, F J

    2016-01-01

    In the last decade, the development of theoretical methods have allowed chemists to reproduce and explain almost all of the experimental data associated with elementary atom plus diatom collisions. However, there are still a few examples where theory cannot account yet for experimental results. This is the case for the preferential population of one of the $\\Lambda$-doublet states produced by chemical reactions. In particular, recent measurements of the OD($^2\\Pi$) product of the O($^3$P) + D$_2$ reaction have shown a clear preference for the $\\Pi(A')$ $\\Lambda$-doublet states, in apparent contradiction with {\\em ab initio} calculations, which predict a larger reactivity on the $A"$ potential energy surface. Here we present a method to calculate the $\\Lambda$-doublet ratio when concurrent potential energy surfaces participate in the reaction. It accounts for the experimental $\\Lambda$-doublet populations via explicit consideration of the stereodynamics of the process. Furthermore, our results demonstrate that...

  14. Superplasticity in an Aluminum Alloy 6061/A12O3p Composite

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The superplasticity of an Al2O3p/6061Al composite, fabricated by powder metallurgy techniques,has been investigated. Instead of any special thermomechanical processing or hot rolling, simple hot extrusion has been employed to obtain a fine grained structure before superplastic testing.Superplastic tensile tests were performed at strain rates ranging from 10-2 to 10-4 s-1 and at temperatures from 833 to 893 K. A maximum elongation of 200% was achieved at a temperature of 853 K and an initial strain rate of 1.67×10-3 s-1. The highest value obtained for the strain rate sensitivity index (m) was 0.32. Differential scanning calorimeter was used to ascertain the possibility of any partial melting in the vicinity of optimum superplastic temperature. These results suggested that no liquid phase existed where maximum elongation was achieved and deformation took place entirely in the solid state.

  15. Functional Diversification of Fungal Glutathione Transferases from the Ure2p Class

    OpenAIRE

    Anne Thuillier; Ngadin, Andrew A.; Cécile Thion; Patrick Billard; Jean-Pierre Jacquot; Eric Gelhaye; Mélanie Morel

    2011-01-01

    The glutathione-S-transferase (GST) proteins represent an extended family involved in detoxification processes. They are divided into various classes with high diversity in various organisms. The Ure2p class is especially expanded in saprophytic fungi compared to other fungi. This class is subdivided into two subclasses named Ure2pA and Ure2pB, which have rapidly diversified among fungal phyla. We have focused our analysis on Basidiomycetes and used Phanerochaete chrysosporium as a model to c...

  16. A Simple FSPN Model of P2P Live Video Streaming System

    OpenAIRE

    Kotevski, Zoran; Mitrevski, Pece

    2011-01-01

    Peer to Peer (P2P) live streaming is relatively new paradigm that aims at streaming live video to large number of clients at low cost. Many such applications already exist in the market, but, prior to creating such system it is necessary to analyze its performance via representative model that can provide good insight in the system’s behavior. Modeling and performance analysis of P2P live video streaming systems is challenging task which requires addressing many properties and issues of P2P s...

  17. Mobility Impact in Initializing Ring-Based P2P Systems over MANETs

    Directory of Open Access Journals (Sweden)

    Wei Ding

    2008-10-01

    Full Text Available With the encouragement from success of P2P systems in real world application, recently we have seen active research on synergy of P2P systems and mobile ad hoc networks. The paper proposes a solution for mobility disturbance problem in initialization of ring-based P2P systems over ad hoc networks. It is a decentralized ring construction protocol in presence of mobility. A Mobile Ring Ad-hoc Networks (MRAN protocol is presented. MRAN is an extension of RAN [1] under the mobile condition. Simulation result shows MRAN works well with mobility. Upper bound of maximum speed of moving nodes is investigated in simulation.

  18. The Number of Solutions of the Diophantine Equation x2 + p2 =yn%Diophantine方程x2+p2=yn的解数

    Institute of Scientific and Technical Information of China (English)

    梁明

    2013-01-01

    Let p be a fixed odd prime,and let N(p) denote the number of positive integer solutions (x,y,n) of the equation x2 +p2 =yn satisfying gcd(x,y)=1 and n > 2.In this paper we prove that if p ≠ 7,then N(p) < log p.%对于给定的奇素数p,设N(p)为方程x2+ p2=yn满足gcd(x,y)=1和n>2的正整数解(x,y,n)的个数,本文证明了:当p≠7时,N(p) <log p.

  19. Overexpression of microRNA‑125a‑3p effectively inhibits the cell growth and invasion of lung cancer cells by regulating the mouse double minute 2 homolog/p53 signaling pathway.

    Science.gov (United States)

    Li, Shenglei; Li, Xin; Zhao, Huasi; Gao, Ming; Wang, Feng; Li, Wencai

    2015-10-01

    MicroRNAs (miRs) are a family of small non-coding RNAs that are 21‑24 nucleotides in length. Decreased expression of hsa‑miR‑125a‑3p is observed in a number of patients with non‑small cell lung cancer; however, it is not clear how this miRNA regulates the growth and invasion of lung tumor cells. The aim of the present study was to identify the function of hsa‑miR‑125a‑3p in the growth and invasion of lung cancer cells. The expression of hsa‑miR‑125a‑3p in the A549, NCI‑H460 and SPCA‑1 lung cancer cell lines was analyzed by reverse transcription‑quantitative polymerase chain reaction and the human bronchiolar epithelium cell line (HBE) was used as a control. The results demonstrated that the expression of hsa‑miR‑125a‑3p was significantly lower in NCI‑H460, A549 and SPCA‑1 cells, compared with that in HBE cells. Overexpression of sense miR‑125a‑3p in the A549 lung cancer cell line inhibited cell proliferation for 5‑7 days (Pcancer cells. In addition, overexpression of miR‑125a‑3p in the NCI‑H460 lung cancer cell line markedly induced cell apoptosis, which was detected by fluorescence‑activated cell sorting with annexin V‑fluorescein isothiocyanate/propidium iodide staining. The results of the Transwell migration assay also revealed that transfection of miR‑125a‑3p resulted in decreased migration of lung cancer tumor cells. The pro‑apoptotic gene p53 expression was detected by western blot analysis. The results revealed that the expression of mouse double minute (MDM)‑2 homolog, the principal cellular antagonist of p53, was decreased and p53 expression was upregulated in sense has‑miR‑125a‑3p transfected A549 cells. This was consistent with that observed in NCI‑H460 cells, suggesting that hsa‑miR‑125a‑3p may be involved in the regulation of the MDM2/p53 signaling pathway in lung cancer cells. In conclusion, overexpression of hsa‑miR‑125a‑3p significantly inhibited the proliferation and

  20. CHARACTERIZATION AND CHROMOSOMAL ASSIGNMENT OF YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN 3P13-P21-SPECIFIC SEQUENCE-TAGGED SITES

    NARCIS (Netherlands)

    MICHAELIS, SC; BARDENHEUER, W; LUX, A; SCHRAMM, A; GOCKEL, A; SIEBERT, R; WILLERS, C; SCHMIDTKE, K; TODT, B; VANDERHOUT, AH; BUYS, CHCM; HEPPELLPARTON, AC; RABBITTS, PH; UNGAR, S; SMITH, D; LEPASLIER, D; COHEN, D; OPALKA, B; SCHUTTE, J

    1995-01-01

    Human chromosomal region 3p12-p23 is proposed to harbor at least three tumor suppressor genes involved in the development of lung cancer, renal cell carcinoma, and other neoplasias. In order to identify one of these genes we defined sequence tagged sites (STSs) specific for 3p13-p24.2 by analyzing a

  1. DEPENDENCE OF ELECTRON-CAPTURE FROM NA-ASTERISK(3P) ON ORBITAL ALIGNMENT - A KINEMATIC EFFECT

    NARCIS (Netherlands)

    SCHLATMANN, AR; MORGENSTERN, R

    1993-01-01

    Recent experimental data of electron capture into He+(4l) states from SIGMA and PI aligned Na*(3p) orbitals are interpreted. Calculations of the overlap integral of the initial Na*(3p) and the final He+(4l) wavefunctions in momentum space reproduce the velocity dependence of the measured cross secti

  2. STRONG VELOCITY DEPENDENCE OF ELECTRON-CAPTURE IN COLLISIONS BETWEEN ALIGNED NA-ASTERISK(3P) AND HE-2+

    NARCIS (Netherlands)

    SCHLATMANN, AR; HOEKSTRA, R; MORGENSTERN, R; OLSON, RE; PASCALE, J

    1993-01-01

    By analyzing spectra of emitted photons, we have studied state-selective electron capture in collisions of He2+ on aligned Na*(3p) atoms that span the ''velocity-matching'' energy between projectile and target electron. We find a strong dependence of the capture cross sections on the Na*(3p) orbital

  3. Experimental Determination of the Lifetime of the Helium 2P13 1S01 Transition

    Science.gov (United States)

    Dall, R. G.; Baldwin, K. G. H.; Byron, L. J.; Truscott, A. G.

    2008-01-01

    We present the first experimental determination of the 2P13 1S01 transition rate in helium and compare this measurement with theoretical quantum-electrodynamic predictions. The experiment exploits the very long (˜1minute) confinement times obtained for atoms magneto-optically trapped in an apparatus used to create a Bose-Einstein condensate of metastable (2S13) helium. The 2P13 1S01 transition rate is measured directly from the decay rate of the cold atomic cloud following 1083 nm laser excitation from the 2S13 to the 2P13 state, and from accurate knowledge of the 2P13 population. The value obtained is 177±8s-1, which agrees very well with theoretical predictions, and has an accuracy that compares favorably with measurements for the same transition in heliumlike ions higher in the isoelectronic sequence.

  4. Pre-Paid Charging System for Sip-P2P Commercial Applications

    Directory of Open Access Journals (Sweden)

    Damian Nowak

    2011-03-01

    Full Text Available The new SIP-P2P approach brings many advantages like the improved reliability and the ease of setup.However, Communications Service Providers (CSPs offering VoIP services seem to be afraid to losetheir profits due to the introduction of SIP-P2P. Claimed is the lack of session establishment controlleading to revenue leakages. In this work we introduce a charging system for SIP-P2P that assures CSPs’revenue from using SIP-based peer-to-peer communications commercially. The proposed solutionconsists of a new “carrier-grade P2P network” concept, software modification of existing equipment andgives some charging application design guidelines. An application prototype was implemented toexamine the concept. Additionally, experiments were done to provide the methodology for systemdimensioning.

  5. Locating Highly Connected Nodes in P2P Networks with Heterogeneous Structures

    Institute of Scientific and Technical Information of China (English)

    ZHANG Haoxiang; ZHANG Lin; SHAN Xiuming; Victor O. K. LI

    2009-01-01

    Peer-to-peer (P2P) networks aggregate enormous storage and processing resources while mini-mizing entry and scaling costs. Gnutella-like P2P networks are complex heterogeneous networks, in which the underlying overlay topology has a power-law node degree distribution. While scale-free networks have great robustness against random failures, they are vulnerable to deliberate attacks where highly connected nodes are eliminated. Since high degree nodes play an important role in maintaining the connectivity, this paper presents an algorithm based on random walks to locate high degree nodes in P2P networks. Simula-tions demonstrate that the algorithm performs well in various scenarios and that heterogeneous P2P net-works are very sensitive to deliberate attacks.

  6. CHC: A Robust P2P Overlay Network with Simple Routing and Small-World Features

    Directory of Open Access Journals (Sweden)

    Wenjun Xiao

    2011-08-01

    Full Text Available Almost all recent researches on P2P systems focus on howto build a highly usable P2P overlay network. Researchersinclude small routing table, short query path and goodrobustness into their design objectives of overlay topology. Inthis paper, we present a general group theory method anddefine a new Cayley graph. Based on this Cayley graph, wepropose a novel P2P overlay network called CHC, which hassimple routing (searching scheme and many other excellentproperties such as short query path, high clustering and goodrobustness because of its symmetry. The performance isevaluated by simulation to show that CHC possesses shorterquery path length and higher clustering and better robustnessthan several popular P2P overlay networks such as Chord andUlysses.

  7. A Local Scalable Distributed EM Algorithm for Large P2P Networks

    Data.gov (United States)

    National Aeronautics and Space Administration — his paper describes a local and distributed expectation maximization algorithm for learning parameters of Gaussian mixture models (GMM) in large peer-to-peer (P2P)...

  8. MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1

    Energy Technology Data Exchange (ETDEWEB)

    Gu, Haihua; Yang, Tao; Fu, Shaozi; Chen, Xiaofan; Guo, Lei; Ni, Yiming, E-mail: ni_yiming@hotmail.com

    2014-01-31

    Highlights: • We examined the level of miR-490-3p in A549 lung cancer cells compared with normal bronchial epithelial cell line. • We are the first to show the function of miR-490-3p in A549 lung cancer cells. • We demonstrate CCND1 may be one of the targets of miR-490-3p. - Abstract: MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate the translation of messenger RNAs by binding their 3′-untranslated region (3′UTR). In this study, we found that miR-490-3p is significantly down-regulated in A549 lung cancer cells compared with the normal bronchial epithelial cell line. To better characterize the role of miR-490-3p in A549 cells, we performed a gain-of-function analysis by transfecting the A549 cells with chemically synthesized miR-490-3P mimics. Overexpression of miR-490-3P evidently inhibits cell proliferation via G1-phase arrest. We also found that forced expression of miR-490-3P decreased both mRNA and protein levels of CCND1, which plays a key role in G1/S phase transition. In addition, the dual-luciferase reporter assays indicated that miR-490-3P directly targets CCND1 through binding its 3′UTR. These findings indicated miR-490-3P could be a potential suppressor of cellular proliferation.

  9. H2S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expression

    Science.gov (United States)

    Li, Xing-Hui; Xue, Wen-Long; Wang, Ming-Jie; Zhou, Yu; Zhang, Cai-Cai; Sun, Chen; Zhu, Lei; Liang, Kun; Chen, Ying; Tao, Bei-Bei; Tan, Bo; Yu, Bo; Zhu, Yi-Chun

    2017-01-01

    The aims of the present study are to determine whether hydrogen sulfide (H2S) is involved in the expression of endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) production, and to identify the role of microRNA-455-3p (miR-455-3p) during those processes. In cultured human umbilical vein endothelial cells (HUVECs), the expression of miR-455-3p, eNOS protein and the NO production was detected after administration with 50 μM NaHS. The results indicated that H2S could augment the expression of miR-455-3p and eNOS protein, leading to the increase of NO level. We also found that overexpression of miR-455-3p in HUVECs increased the protein levels of eNOS whereas inhibition of miR-455-3p decreased it. Moreover, H2S and miR-455-3p could no longer increase the protein level of eNOS in the presence of proteasome inhibitor, MG-132. In vivo, miR-455-3p and eNOS expression were considerably increased in C57BL/6 mouse aorta, muscle and heart after administration with 50 μmol/kg/day NaHS for 7 days. We also identified that H2S levels and miR-455-3p expression increased in human atherosclerosis plaque while H2S levels decreased in plasma of atherosclerosis patients. Our data suggest that the stability of eNOS protein and the NO production could be regulated by H2S through miR-455-3p. PMID:28322298

  10. Secure Mobile Agent for Telemedicine Based on P2P Networks

    OpenAIRE

    Hsu, Wen-Shin; Pan, Jiann-I

    2013-01-01

    Exploring intelligent mobile agent (MA) technology for assisting medical services or transmitting personal patient-health information in telemedicine applications has been widely investigated. Conversely, peer-to-peer (P2P) networking has become one of the most popular applications used in the Internet because of its benefits for easy-to-manage resources and because it balances workloads. Therefore, constructing an agent-based telemedicine platform based on P2P networking architecture is nece...

  11. First-order ferromagnetic transition in single-crystalline (Mn,Fe)(2)( P,Si)

    NARCIS (Netherlands)

    Yibole, H.; Guillou, F.; Huang, Y. K.; Blake, G. R.; Lefering, A. J. E.; van Dijk, N. H.; Bruck, E.

    2015-01-01

    (Mn,Fe)(2)(P, Si) single crystals have been grown by flux method. Single crystal X-ray diffraction demonstrates that Mn0.83Fe1.17P0.72Si0.28 crystallizes in a hexagonal Fe2P crystal structure (space group P (6) over bar 2m) at both 100 and 280 K, in the ferromagnetic and paramagnetic states,

  12. Quasi-elastic scattering, RPA, 2p2h and neutrino--energy reconstruction

    CERN Document Server

    Nieves, J; Sanchez, F; Vacas, M J Vicente

    2013-01-01

    We discuss some nuclear effects, RPA correlations and 2p2h (multinucleon) mechanisms, on charged-current neutrino-nucleus reactions that do not produce a pion in the final state. We study a wide range of neutrino energies, from few hundreds of MeV up to 10 GeV. We also examine the influence of 2p2h mechanisms on the neutrino energy reconstruction.

  13. An Efficient, Scalable and Robust P2P Overlay for Autonomic Communication

    Science.gov (United States)

    Li, Deng; Liu, Hui; Vasilakos, Athanasios

    The term Autonomic Communication (AC) refers to self-managing systems which are capable of supporting self-configuration, self-healing and self-optimization. However, information reflection and collection, lack of centralized control, non-cooperation and so on are just some of the challenges within AC systems. Since many self-* properties (e.g. selfconfiguration, self-optimization, self-healing, and self-protecting) are achieved by a group of autonomous entities that coordinate in a peer-to-peer (P2P) fashion, it has opened the door to migrating research techniques from P2P systems. P2P's meaning can be better understood with a set of key characteristics similar to AC: Decentralized organization, Self-organizing nature (i.e. adaptability), Resource sharing and aggregation, and Fault-tolerance. However, not all P2P systems are compatible with AC. Unstructured systems are designed more specifically than structured systems for the heterogeneous Internet environment, where the nodes' persistence and availability are not guaranteed. Motivated by the challenges in AC and based on comprehensive analysis of popular P2P applications, three correlative standards for evaluating the compatibility of a P2P system with AC are presented in this chapter. According to these standards, a novel Efficient, Scalable and Robust (ESR) P2P overlay is proposed. Differing from current structured and unstructured, or meshed and tree-like P2P overlay, the ESR is a whole new three dimensional structure to improve the efficiency of routing, while information exchanges take in immediate neighbors with local information to make the system scalable and fault-tolerant. Furthermore, rather than a complex game theory or incentive mechanism, asimple but effective punish mechanism has been presented based on a new ID structure which can guarantee the continuity of each node's record in order to discourage negative behavior on an autonomous environment as AC.

  14. P2P network traffic control mechanism based on global evaluation values

    Institute of Scientific and Technical Information of China (English)

    XU Xiao-long; WANG Ru-chuan

    2009-01-01

    Peer-to-peer (P2P) computing technology has been widely used on the Internet to exchange data. However, it occupies much network bandwidth, and thus greatly influences traditional business on the Interact. Besides, problems about free-riders and 'tragedy of the commons' in the P2P environment estrange from it P2P users who constantly contribute to the network with quality resources. This article proposes a new P2P network traffic control mechanism based on global evaluation values. It aims to help individual users to avoid peak traffic time as much as possible, ease network congestion and protect traditional business on the Interact, as well as differentiating priority grades of peers according to their contributions and stimulating them to share their valuable resources actively. This article first analyzes the current state of network traffic, and then elaborates on P2P network traffic control policies and proposes the peer's priority level differentiation mechanism based on global evaluation values. Finally,after the testing results and analysis of the proposed P2P network traffic control mechanism are discussed, conclusions are drawn.

  15. Fine-grained P2P traffc classifi cation by simply counting flows

    Institute of Scientific and Technical Information of China (English)

    Jie HE; Yue-xiang YANG; Yong QIAO; Wen-ping DENG

    2015-01-01

    The continuous emerging of peer-to-peer (P2P) applications enriches resource sharing by networks, but it also brings about many challenges to network management. Therefore, P2P applications monitoring, in particular, P2P traffic classifi cation, is becoming increasingly important. In this paper, we propose a novel approach for accurate P2P traffic classifi cation at a fi ne-grained level. Our approach relies only on counting some special fl ows that are appearing frequently and steadily in the traffic generated by specifi c P2P applications. In contrast to existing methods, the main contribution of our approach can be summarized as the following two aspects. Firstly, it can achieve a high classifi cation accuracy by exploiting only several generic properties of fl ows rather than complicated features and sophisticated techniques. Secondly, it can work well even if the classifi cation target is running with other high bandwidth-consuming applications, outperforming most existing host-based approaches, which are incapable of dealing with this situation. We evaluated the performance of our approach on a real-world trace. Experimental results show that P2P applications can be classifi ed with a true positive rate higher than 97.22%and a false positive rate lower than 2.78%.

  16. Strategies for P2P connectivity in reconfigurable converged wired/wireless access networks.

    Science.gov (United States)

    Puerto, Gustavo; Mora, José; Ortega, Beatriz; Capmany, José

    2010-12-06

    This paper presents different strategies to define the architecture of a Radio-Over-Fiber (RoF) Access networks enabling Peer-to-Peer (P2P) functionalities. The architectures fully exploit the flexibility of a wavelength router based on the feedback configuration of an Arrayed Waveguide Grating (AWG) and an optical switch to broadcast P2P services among diverse infrastructures featuring dynamic channel allocation and enabling an optical platform for 3G and beyond wireless backhaul requirements. The first architecture incorporates a tunable laser to generate a dedicated wavelength for P2P purposes and the second architecture takes advantage of reused wavelengths to enable the P2P connectivity among Optical Network Units (ONUs) or Base Stations (BS). While these two approaches allow the P2P connectivity in a one at a time basis (1:1), the third architecture enables the broadcasting of P2P sessions among different ONUs or BSs at the same time (1:M). Experimental assessment of the proposed architecture shows approximately 0.6% Error Vector Magnitude (EVM) degradation for wireless services and 1 dB penalty in average for 1 x 10(-12) Bit Error Rate (BER) for wired baseband services.

  17. Monitoring and Resource Management in P2P Grid-Based Web Services

    Directory of Open Access Journals (Sweden)

    Djafri Laouni

    2012-12-01

    Full Text Available Grid computing has recently emerged as a response to the growing demand for resources (processing power, storage, etc. exhibited by scientific applications. However, as grid sizes increase, the need for self-organization and dynamic reconfigurations is becoming more and more important. Since such properties are exhibited by P2P systems, the convergence of grid computing and P2P computing seems natural. However, using P2P systems (usually running on the Internet on a grid infrastructure (generally available as a federation of SAN-based clusters interconnected by high-bandwidth WANs may raise the issue of the adequacy of the P2P communication mechanisms. Among the interesting properties of P2P systems is the  volatility of  peers  which  causes  the  need  for  integration  of  a  service fault tolerance. And service Load balancing,   As a solution, we proposed a mechanism of fault tolerance and model of Load balancing  adapted to a grid P2P model, named SGRTE (Monitoring and Resource Management, Fault Tolerances and Load Balancing.

  18. Multifunctional g3p-peptide tag for current phage display systems.

    Science.gov (United States)

    Beckmann, C; Haase, B; Timmis, K N; Tesar, M

    1998-03-15

    We have previously described a monoclonal antibody (mAb), 10C3, directed against the gene-3 protein (g3p) of filamentous phage M13, which was produced to study g3p fusion protein expression in Escherichia coli and its incorporation in the phage capsid [Tesar, M., Beckmann, C., Röttgen, P., Haase, B., Faude, U., Timmis, K., 1995. Monoclonal antibody against pIII of filamentous phage: an immunological tool to study pIII fusion protein expression in phage display systems. Immunology 1, 53-54]. In this study we report mapping of the antigenic epitope of the mAb 10C3, by means of short overlapping peptide-sequences [Frank, R., Overwin, H., 1996. Spot synthesis. In: Morris, G.E. (Ed.), Methods in Molecular Biology, Vol. 66: Epitope Mapping Protocols. Humana Press, Totowa, NJ, pp. 149-169.] comprising the C-terminal half of the g3-protein. A minimal recognizable peptide was found which is represented in the 11 amino acid sequence from positions 292 to 302 of g3p [Wezenbeek van, P.M.G.P., Hulsebos, T.J.M., Schoenmakers, J.G.G., 1980. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd. Gene 11, 129-148]. In order to use the antibody also for detection and purification of recombinant proteins, such as single chain antibodies, the epitope was introduced as a tag sequence into the phagemid pHEN1 [Hoogenboom, H.R., Griffith, A.D., Johnson, K., Chiswell, D.J., Hudson, P., Winter, G., 1991. Multi-subunit proteins on the surface of the filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acid Res. 19, 4133-4137; Nissim, A., Hoogenboom, H.R., Tomlinson, I.M., Flynn, G., Midgley, C., Lane, D., Winter, G., 1994. Antibody fragments from a single pot phage display library as immunochemical reagents. EMBO J. 13 (3) 692-698]. Purified single chain antibodies containing this tag were detectable down to a concentration of 2 ng ml(-1) under non-denaturing conditions (ELISA) or 4 ng per lane on immunoblots

  19. miR-409-3p sensitizes colon cancer cells to oxaliplatin by inhibiting Beclin-1-mediated autophagy.

    Science.gov (United States)

    Tan, Shifan; Shi, Huijuan; Ba, Mingchen; Lin, Shengqv; Tang, Hongsheng; Zeng, Xiaoqi; Zhang, Xiangliang

    2016-04-01

    The chemoresistance of colon cancer cells limits the efficacy of chemotherapy. miR-409-3p has been shown to be downregulated in various types of cancer. In the present study, we examined the role of miR-409-3p in colon cancer as well as the effects of miR‑409-3p on the sensitivity of colon cancer cells to oxaliplatin. The expression of miR-409 was significantly downregulated in the human colon cancer cell lines compared with the normal colon epithelial cells. Importantly, the miR-409-3p expression levels were lower in human colon cancer patient samples than in normal colon tissues. Moreover, we observed a negative correlation between the miR‑409-3p levels and resistance to oxaliplatin: the oxaliplatin-resistant colon cancer cells exhibited significantly downregulated miR‑409-3p levels, but higher autophagic activity than the oxaliplatin-sensitive cells. Using bioinformatics analysis, we predicted that miR‑409-3p miRNA binds to the key autophagy gene encoding Beclin-1. Our findings indicated that the overexpression of miR‑409-3p inhibited Beclin-1 expression and autophagic activity by binding to the 3'-untranslated region of Beclin-1 mRNA. In addition, the overexpression of miR‑409-3p enhanced the chemosensitivity of the oxaliplatin-sensitive and oxaliplatin-resistant colon cancer cells. The restoration of Beclin-1 abrogated these effects of miR‑409-3p. In a xenograft model using nude mice, we examined the effects of miR‑409-3p on tumor growth during chemotherapy. miR‑409-3p overexpression sensitized the tumor to chemotherapy, while inhibiting chemotherapy-induced autophagy in a manner dependent on Beclin-1. The findings of our study suggest that miR-409-3p is capable of enhancing the chemosensitivity of colon cancer cells by inhibiting Beclin-1-mediated autophagy.

  20. P2P-based botnets: structural analysis, monitoring, and mitigation

    Energy Technology Data Exchange (ETDEWEB)

    Yan, Guanhua [Los Alamos National Laboratory; Eidenbenz, Stephan [Los Alamos National Laboratory; Ha, Duc T [UNIV AT BUFFALO; Ngo, Hung Q [UNIV AT BUFFALO

    2008-01-01

    Botnets, which are networks of compromised machines that are controlled by one or a group of attackers, have emerged as one of the most serious security threats on the Internet. With an army of bots at the scale of tens of thousands of hosts or even as large as 1.5 million PCs, the computational power of botnets can be leveraged to launch large-scale DDoS (Distributed Denial of Service) attacks, sending spamming emails, stealing identities and financial information, etc. As detection and mitigation techniques against botnets have been stepped up in recent years, attackers are also constantly improving their strategies to operate these botnets. The first generation of botnets typically employ IRC (Internet Relay Chat) channels as their command and control (C&C) centers. Though simple and easy to deploy, the centralized C&C mechanism of such botnets has made them prone to being detected and disabled. Against this backdrop, peer-to-peer (P2P) based botnets have emerged as a new generation of botnets which can conceal their C&C communication. Recently, P2P networks have emerged as a covert communication platform for malicious programs known as bots. As popular distributed systems, they allow bots to communicate easily while protecting the botmaster from being discovered. Existing work on P2P-based hotnets mainly focuses on measurement of botnet sizes. In this work, through simulation, we study extensively the structure of P2P networks running Kademlia, one of a few widely used P2P protocols in practice. Our simulation testbed incorporates the actual code of a real Kademlia client software to achieve great realism, and distributed event-driven simulation techniques to achieve high scalability. Using this testbed, we analyze the scaling, reachability, clustering, and centrality properties of P2P-based botnets from a graph-theoretical perspective. We further demonstrate experimentally and theoretically that monitoring bot activities in a P2P network is difficult

  1. miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200

    Science.gov (United States)

    Fang, Wang; Shu, Shan; Yongmei, Li; Endong, Zhu; Lirong, Yin; Bei, Sun

    2016-01-01

    Cervical cancer (CC) is a malignant solid tumor, which is one of the main causes of morbidity and mortality in women. Persistent High-risk human papillomavirus (hrHPV) infection is closely related to cervical cancer and autophagy has been suggested to inhibit viral infections. miRNAs have been reported to regulate autophagy in many solid tumors with many studies implicating miR-224-3p in the regulation of autophagy. In this study, we performed a miRNA microarray analysis on CC tissues and found that a large number of miRNAs with differential expressions in hrHPV-infected tissues. We identified miR-224-3p as a candidate miRNA selectively up regulated in HPV-infected tissues and cell lines. Further analysis revealed that miR-224-3p regulates autophagy in cervical cancer tissues and cell lines. While the overexpression of miR-224-3p inhibits autophagy in HPV-infected cells, knocking down endogenous miR-224-3p increases autophagy activity in the same cells. In addition, we found that miR-224-3p directly inhibits the expression of autophagy related gene, FAK family-interacting protein of 200 kDa (FIP200). In summary, we found that miR-224-3p regulates autophagy in hrHPV-induced cervical cancer cells through targeting FIP200 expression. PMID:27615604

  2. miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells

    Directory of Open Access Journals (Sweden)

    Zhong-Yan Chen

    2017-01-01

    Full Text Available MicroRNAs (miRNAs play important roles in cell fate decisions. However, the miRNAs and their targets involved in the regulation of cardiac lineage specification are largely unexplored. Here, we report novel functions of miR-142-3p in the regulation of cardiomyocyte differentiation from mouse embryonic stem cells (mESCs. With a miRNA array screen, we identified a number of miRNAs significantly changed during mESC differentiation into the mesodermal and cardiac progenitor cells, and miR-142-3p was one among the markedly downregulated miRNAs. Ectopic expression and inhibition of miR-142-3p did not alter the characteristics of undifferentiated ESCs, whereas ectopic expression of miR-142-3p impaired cardiomyocyte formation. In addition, ectopic expression of miR-142-3p inhibited the expression of a cardiac mesodermal marker gene Mesp1 and downstream cardiac transcription factors Nkx2.5, Tbx5, and Mef2c but not the expression of three germ layer-specific genes. We further demonstrated that miR-142-3p targeted the 3′-untranslated region of Mef2c. These results reveal miR-142-3p as an important regulator of early cardiomyocyte differentiation. Our findings provide new knowledge for further understanding of roles and mechanisms of miRNAs as critical regulators of cardiomyocyte differentiation.

  3. MicroRNA-338-3p suppresses tumor growth of esophageal squamous cell carcinoma in vitro and in vivo.

    Science.gov (United States)

    Li, Xinyu; Li, Zhihong; Yang, Guiyun; Pan, Zhenxiang

    2015-09-01

    Accumulating evidence has shown that microRNAs (miRNAs) are aberrantly expressed in human esophageal squamous cell carcinoma (ESCC) and are crucial in tumorigenesis, among which miR‑338‑3p has been examined to be downregulated in patients with ESCC. However, the role of miR‑338‑3p in ESCC remains to be elucidated. In the present study, the role of miR‑338‑3p on the growth and survival of an ESCC cell line was determined with several in vitro approaches and in nude mouse models. It was determined that miR‑338‑3p expression was frequently downregulated in ESCC tissue compared with corresponding adjacent non‑tumor tissue, and that its expression was significantly correlated with tumor stage and metastasis. Overexpression of miR‑338‑3p in ESCC cells suppressed cell proliferation, colony formation, migration and invasion, and induced cell arrest at the G0/G1 stage and cell apoptosis in vitro. In addition, it was demonstrated that overexpression of miR‑338‑3p significantly suppresses tumor growth of xenograft tumors in mice (PESCC, and its dysregulation may be involved in the initiation and development of human ESCC. In addition, it was suggested that miR‑338‑3p may be a potential therapeutic agent for treatment of ESCC.

  4. Functional expression of pig renal organic anion transporter 3 (pOAT3).

    Science.gov (United States)

    Hagos, Yohannes; Braun, Isabella M; Krick, Wolfgang; Burckhardt, Gerhard; Bahn, Andrew

    2005-05-01

    With the cloning of pig renal organic anion transporter 1 (pOAT1) (Biochimie 84 (2002) 1219) we set up a model system for comparative studies of cloned and natively isolated membrane located transport proteins. Meanwhile, another transport protein involved in p-aminohippurate (PAH) uptake on the basolateral side of the proximal tubule cells was identified, designated organic anion transporter 3 (OAT3). To explore the contribution of pOAT1 to the PAH clearance in comparison to OAT3, it was the aim of this study to extend our model by cloning of the pig ortholog of OAT3. Sequence comparisons of human organic anion transporter 3 (hOAT3) with the expressed sequence tag (EST) database revealed a clone and partial sequence of the pig renal organic anion transporter 3 (pOAT3) ortholog. Sequencing of the entire open reading frame resulted in a protein of 543 amino acid residues encoded by 1632 base pairs (EMBL Acc. No. AJ587003). It showed high homologies of 81%, 80%, 76%, and 77% to the human, rabbit, rat, and mouse OAT3, respectively. A functional characterization of pOAT3 in Xenopus laevis oocytes yielded an apparent Km (Kt) for [3H]estrone sulfate of 7.8 +/- 1.3 microM. Moreover, pOAT3 mediated [3H]estrone sulfate uptake was almost abolished by 0.5 mM of glutarate, dehydroepiandosterone sulfate, or probenecid consistent with the hallmarks of OAT3 function.

  5. Excitons into one-axis crystals of zinc phosphide (Zn3P2

    Directory of Open Access Journals (Sweden)

    D.M. Stepanchikov

    2009-01-01

    Full Text Available Theoretical study of excitons spectra is offered in this report as for Zn3P2 crystals. Spectra are got in the zero approach of the theory of perturbations with consideration of both the anisotropy of the dispersion law and the selection rules. The existence of two exciton series was found, which corresponds to two valence bands (hh, lh and the conductivity band (c. It is noteworthy that anisotropy of the dispersion law plus the existence of crystalline packets (layers normal to the main optical axis, both will permit the consideration of two-dimensional excitons too. The high temperature displaying of these 2D-exciton effects is not eliminated even into bulk crystals. The calculated values of the binding energies as well as the oscillator's strength for the optical transitions are given for a volume (3D and for two-dimensional (2D excitons. The model of energy exciton transitions and four-level scheme of stimulated exciton radiation for receiving laser effect are offered.

  6. Dynamics of the gas-liquid interfacial reaction of O(3P) atoms with hydrocarbons

    Science.gov (United States)

    Kelso, Hailey; Köhler, Sven P. K.; Henderson, David A.; McKendrick, Kenneth G.

    2003-11-01

    We describe an experimental approach to the determination of the nascent internal state distribution of gas-phase products of a gas-liquid interfacial reaction. The system chosen for study is O(3P) atoms with the surface of liquid deuterated squalane, a partially branched long-chain saturated hydrocarbon, C30D62. The nascent OD products are detected by laser-induced fluorescence. Both OD (v'=0) and (v'=1) were observed in significant yield. The rotational distributions in both vibrational levels are essentially the same, and are characteristic of a Boltzmann distribution at a temperature close to that of the liquid surface. This contrasts with the distributions in the corresponding homogeneous gas-phase reactions. We propose a preliminary interpretation in terms of a dominant trapping-desorption mechanism, in which the OD molecules are retained at the surface sufficiently long to cause rotational equilibration but not complete vibrational relaxation. The significant yield of vibrationally excited OD also suggests that the surface is not composed entirely of -CD3 endgroups, but that secondary and/or tertiary units along the backbone are exposed.

  7. Theoretical investigation of hyperthermal reactions at the gas-liquid interface: O (3P) and squalane.

    Science.gov (United States)

    Kim, Dongwook; Schatz, George C

    2007-06-14

    Hyperthermal collisions (5 eV) of ground-state atomic oxygen [O ((3)P)] with a liquid-saturated hydrocarbon, squalane (C(30)H(62)), have been studied using QM/MM hybrid "on-the-fly" direct dynamics. The surface structure of the liquid squalane is obtained from a classical molecular dynamics simulation using the OPLS-AA force field. The MSINDO semiempirical Hamiltonian is combined with OPLS-AA for the QM/MM calculations. In order to achieve a more consistent and efficient simulation of the collisions, we implemented a dynamic partitioning of the QM and MM atoms in which atoms are assigned to QM or MM regions based on their proximity to "seed" (open-shell) atoms that determine where bond making/breaking can occur. In addition, the number of seed atoms is allowed to increase or decrease as time evolves so that multiple reactive events can be described. The results show that H abstraction is the most important process for all incident angles, with H elimination, double H abstraction, and C-C bond cleavage also being important. A number of properties of these reactive channels, as well as inelastic nonreactive scattering, are investigated, including angular and translational energy distributions, the effect of incident collision angle, variation with depth of the reactive event within the liquid, with the reaction site on the hydrocarbon, and the effect of dynamics before and after reaction (direct reaction versus trapping reaction-desorption).

  8. Dynamics of interfacial reactions between O(3 P) atoms and long-chain liquid hydrocarbons

    Science.gov (United States)

    Allan, Mhairi; Bagot, Paul A. J.; Köhler, Sven P. K.; Reed, Stewart K.; Westacott, Robin E.; Costen, Matthew L.; McKendrick, Kenneth G.

    2007-09-01

    Recent progress that has been made towards understanding the dynamics of collisions at the gas-liquid interface is summarized briefly. We describe in this context a promising new approach to the experimental study of gas-liquid interfacial reactions that we have introduced. This is based on laser-photolytic production of reactive gas-phase atoms above the liquid surface and laser-spectroscopic probing of the resulting nascent products. This technique is illustrated for reaction of O(3P) atoms at the surface of the long-chain liquid hydrocarbon squalane (2,6,10,15,19,23-hexamethyltetracosane). Laser-induced fluorescence detection of the nascent OH has revealed mechanistically diagnostic correlations between its internal and translational energy distributions. Vibrationally excited OH molecules are able to escape the surface. At least two contributions to the product rotational distributions are identified, confirming and extending previous hypotheses of the participation of both direct and trapping-desorption mechanisms. We speculate briefly on future experimental and theoretical developments that might be necessary to address the many currently unanswered mechanistic questions for this, and other, classes of gas-liquid interfacial reaction.

  9. Join the CERN ISEF special award winners | 16 June - 3 p.m.

    CERN Multimedia

    2016-01-01

    Come and join the CERN ISEF special award winners at their lightning talks session on 16 June at 3.00 p.m. in the main auditorium.   The 2016 Intel ISEF CERN special award winners on stage with the selection committee on 17 May 2016 in Phoenix, Arizona, USA. (Picture: Society for Science and the Public) Between 11 and 17 June 2016, the ten finalists of the Intel International Science and Engineering Fair (ISEF) who won the CERN Special Award, will visit CERN to partake in various educational lectures. ISEF is the world's largest international pre-college science competition, with approximately 1,700 high school students from more than 75 countries taking part. They will present their projects in short 5 minutes lightning talks' sessions at the main auditorium on Thursday 16 June at 3 p.m. The award winners would be also very happy to have a chance to interact and discuss with you af...

  10. Utilising the `3P-model' to Characterise the Discipline of Didactics of Science

    Science.gov (United States)

    Adúriz-Bravo, Agustín; Izquierdo-Aymerich, Mercè

    In our research within didactics of science, we have been exploring contributions of the so called cognitive models from contemporary philosophy of science. We have used these philosophical frameworks on different levels. As an outcome, we have formulated a model of didactics of science according to which this discipline adapts and transforms theoretical contributions from different scholarly fields. In this paper, we concentrate on this description of didactics of science, which we have called the 3P-model (i.e., philosophy + psychology + pedagogy). This model of the internal functioning of the discipline may be useful to make innovations in science curriculum design and re-conceptualise the role of science teachers as professionals. We see didactics of science as a set of interrelated activities, performed by different individuals, and ranging from theoretical production to practice of science education at school. We find the concept of technoscience suitable to account for this diversity of goals. According to this concept, scientific disciplines are identified both with generation of knowledge and with active intervention on the world. Within current didactics of science, we recognise several kinds of research, having goals more or less directed to practical intervention in science education.

  11. Precision frequency measurement of 1S0-3P1 intercombination lines of Sr isotopes

    Science.gov (United States)

    Liu, Hui; Gao, Feng; Ye-Bing, Wang; Xiao, Tian; Jie, Ren; Ben-Quan, Lu; Qin-Fang, Xu; Yu-Lin, Xie; Hong, Chang

    2015-01-01

    We report on frequency measurement of the intercombination (5s2)1S0-(5s5p)3P1 transition of the four natural isotopes of strontium, including 88Sr (82.58%), 87Sr (7.0%), 86Sr (9.86%), and 84Sr (0.56%). A narrow-linewidth laser that is locked to an ultra-low expansion (ULE) optical cavity with a finesse of 12000 is evaluated at a linewidth of 200 Hz with a fractional frequency drift of 2.8×10-13 at an integration time of 1 s. The fluorescence collector and detector are specially designed, based on a thermal atomic beam. Using a double-pass acousto-optic modulator (AOM) combined with a fiber and laser power stabilization configuration to detune the laser frequency enables high signal-to-noise ratios and precision saturated spectra to be obtained for the six transition lines, which allows us to determine the transition frequency precisely. The optical frequency is measured using an optical frequency synthesizer referenced to an H maser. Both the statistical values and the final values, including the corrections and uncertainties, are derived for a comparison with the values given in other works. Project supported by the National Natural Science Foundation of China (Grant No. 61127901) and the Key Project of the Chinese Academy of Sciences (Grant No. KJZD-EW-W02).

  12. miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Peng; Wu, Dingguo; You, Yu; Sun, Jing; Lu, Lele; Tan, Jiaxing; Bie, Ping, E-mail: bieping2010@163.com

    2015-08-15

    MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at post-transcriptional level. miRNA dysregulation plays a causal role in cancer progression. In this study, miR-208-3p was highly expressed and directly repressed ARID2 expression. As a result, ARID2 expression in hepatocellular carcinoma (HCC) was decreased. In vitro, miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. In vivo test results revealed that miR-208-3p down-regulation inhibited HCC tumorigenesis in Hep3B cells. Moreover, ARID2 was possibly a downstream element of transforming growth factor beta1 (TGFβ1)/miR-208-3p/ARID2 regulatory pathway. These findings suggested that miR-208-3p up-regulation is associated with HCC cell progression and may provide a new target for liver cancer treatment. - Highlights: • miR-208-3p was highly expressed and directly repressed the expression of ARID2 in HCC. • miR-208-3p contributed to HCC cell progression both in vitro and in vivo. • Over-expression of ARID2 inhibited the HCC cell proliferation and invasion. • Restoration of ARID2 partly reversed the the effect of miR-208-3p down-regulation on HCC cells. • Newly regulatory pathway: miR-208-3p mediated the repression of ARID2 by TGFβ1 in HCC cells.

  13. EuCo2P2 : A model molecular-field helical Heisenberg antiferromagnet

    Science.gov (United States)

    Sangeetha, N. S.; Cuervo-Reyes, Eduardo; Pandey, Abhishek; Johnston, D. C.

    2016-07-01

    The metallic compound EuCo2P2 with the body-centered tetragonal ThCr2Si2 structure containing Eu spins-7/2 was previously shown from single-crystal neutron diffraction measurements to exhibit a helical antiferromagnetic (AFM) structure below TN=66.5 K with the helix axis along the c axis and with the ordered moments aligned within the a b plane. Here we report crystallography, electrical resistivity, heat capacity, magnetization, and magnetic susceptibility measurements on single crystals of this compound. We demonstrate that EuCo2P2 is a model molecular-field helical Heisenberg antiferromagnet from comparisons of the anisotropic magnetic susceptibility χ , high-field magnetization, and magnetic heat capacity of EuCo2P2 single crystals at temperature T ≤TN with the predictions of our recent formulation of molecular-field theory. Values of the Heisenberg exchange interactions between the Eu spins are derived from the data. The low-T magnetic heat capacity ˜T3 arising from spin-wave excitations with no anisotropy gap is calculated and found to be comparable to the lattice heat capacity. The density of states at the Fermi energy of EuCo2P2 and the related compound BaCo2P2 are found from the heat capacity data to be large, 10 and 16 states/eV per formula unit for EuCo2P2 and BaCo2P2 , respectively. These values are enhanced by a factor of ˜2.5 above those found from DFT electronic structure calculations for the two compounds. The calculations also find ferromagnetic Eu-Eu exchange interactions within the a b plane and AFM interactions between Eu spins in nearest- and next-nearest planes, in agreement with the MFT analysis of χa b(T ≤TN) .

  14. Measurement of Integrated Stokes Parameters for He 3 3p State Excited by Spin-Polarized Electrons

    Institute of Scientific and Technical Information of China (English)

    DING Hai-Bing; PANG Wen-Ning; LIU Yi-Bao; SHANG Ren-Cheng

    2005-01-01

    @@ Integrated Stokes parameters Pi (i = 1, 2, 3) for the He 3 3p → 2 3S1 (388.9nm) transition after excitation from the ground state to the 3 3 P state by a transversely spin-polarized electron beam are measured in near threshold energy region. The experimental results are presented. The linear-polarization P2 are consistent with zero over the incident energy range, providing evidence for the LS coupling mechanism of the 3 3P state. The measured circular polarization P3 are non-zero, indicating strong electron-electron exchange effects in the spin-polarized electron-atom collision process.

  15. RESEARCH ON GROUPING STRATEGY OF SIP-BASED STREAMING MEDIA P2P LIVE BROADCAST NETWORK

    Institute of Scientific and Technical Information of China (English)

    Liu Hui; Huang Yongfeng; Li Xing

    2008-01-01

    The rapid development of Internet has led to the explosion of information sharing, and how to supervise the sharing is a main research topic on current Internet. Aiming at the disadvantage that the current Peer-to-Peer (P2P) is hard to manage and control, this paper presents a Session Initial Protocol (SIP)-based P2P network of three-level architecture. SIP middleware is introduced to the middle level of the three-layer architecture. By the connection function of the SIP signaling, the P2P transmission on media-level can be controlled. Using SIP's register and authentication function, the manage layer can manage the whole P2P network. Based on the aforementioned architecture, this paper investigates the grouping strategy on a live broadcast application in P2P network. Combined with the function of SIP register, the paper works on several grouping strategies, sets up models to manage users by grouping them, presents a weight-based K-means IP address grouping algorithm, and realizes it. The experiment shows that the grouping strategy presented in this paper can solve the problem of group sharing of network resource, and can realize the efficient-sharing, reasonable-distributing of network resource.

  16. Secure mobile agent for telemedicine based on P2P networks.

    Science.gov (United States)

    Hsu, Wen-Shin; Pan, Jiann-I

    2013-06-01

    Exploring intelligent mobile agent (MA) technology for assisting medical services or transmitting personal patient-health information in telemedicine applications has been widely investigated. Conversely, peer-to-peer (P2P) networking has become one of the most popular applications used in the Internet because of its benefits for easy-to-manage resources and because it balances workloads. Therefore, constructing an agent-based telemedicine platform based on P2P networking architecture is necessary. The main purpose of this paper is to construct a safe agent-based telemedicine that based on P2P networking architecture. Two themes are addressed in this paper: (a) the P2P network architecture for an agent-based telemedicine service, and (b) the security mechanisms for the proposed telemedicine networking architecture. When an MA contains patient information and migrates from one host to another through the Internet, it can be attacked by other software agents or agent platforms that can illegally access patient information. The proposed P2P network architecture is based on the JXTA protocol and provides two types of telemedicine service models: the predictable service model and unpredictable service model. This architecture employs a two-layer safety mechanism for MAs (i.e., time-limited black boxes and RSA undetachable signature technologies), to provide a secure solution for agent-based telemedicine services.

  17. Synthesis, characterization and photocatalytic performance of PbS/Ni2P flowers

    Science.gov (United States)

    Liu, Shuling; Han, Lefang; Liu, Hui

    2016-11-01

    Flower-like PbS/Ni2P composites were synthesized by a facile two-step chemical route. The morphology and structure of the resulting composites were investigated by SEM and TEM images and XRD spectra, respectively. The results showed that the as-obtained composites were composed of the cubic PbS flowers and hexagonal Ni2P nanoparticles, and Ni2P nanoparticles coated on the surfaces of flower-like PbS microstructure. It was found that changing the molar ratio to 3:1, flower-like PbS/Ni2P composites have been successfully synthesized by using cationic cetyltrimethylammonium bromide(CTAB) and anionic sodium dodecyl sulfate (SDS) as template. Furthermore, using methylene blue(MB) as a model organic pollutant, the photocatalytic degradation experiments indicated that the as-prepared composites showed enhanced photocatalytic degradation activity for methylene blue(75%) which is as higher as that of the only flower-like PbS (16.6%) and only Ni2P nanoparticles (44.8%) at the same time. This work may be expected to find its potential application in water pollution treatment.

  18. P2P Streaming Traffic Classification in High-Speed Networks

    Institute of Scientific and Technical Information of China (English)

    Chen Luying; Cong Rong; Yang Jie; Yu Hua

    2011-01-01

    The growing P2P streaming traffic brings a variety of problems and challenges to ISP networks and service providers.A P2P streaming traffic classification method based on sampling technology is presented in this paper.By analyzing traffic statistical features and network behavior of P2P streaming,a group of flow characteristics were found,which can make P2P streaming more recognizable among other applications.Attributes from Netflow and those proposed by us are compared in terms of classification accuracy,and so are the results of different sampling rates.It is proved that the unified classification model with the proposed attributes can identify P2P streaming quickly and efficiently in the online system.Even with 1∶50 sampling rate,the recognition accuracy can be higher than 94%.Moreover,we have evaluated the CPU resources,storage capacity and time consumption before and after the sampling,it is shown that the classification model after the sampling can significantly reduce the resource requirements with the same recognition accuracy.

  19. 2P/Encke, the Taurid complex NEOs and the Maribo and Sutter's Mill meteorites

    CERN Document Server

    Tubiana, C; Michelsen, R; Haack, H; Boehnhardt, H; Fitzsimmons, A; Williams, I P

    2015-01-01

    The Taurid meteoroid stream has long been linked with 2P/Encke owing to a good match of their orbital elements, even though the comet's activity is not strong enough to explain the number of observed meteors. Various small NEOs have been discovered with orbits that can be linked to 2P and the Taurid meteoroid stream. Maribo and Sutter's Mill are CM type carbonaceous chondrites that fell in Denmark on Jan 17, 2009 and Apr 22, 2012, respectively. Their pre-atmospheric orbits place them in the middle of the Taurid meteoroid stream, which raises the intriguing possibility that comet 2P could be the parent body of CM chondrites. To investigate whether a relationship between comet 2P, the Taurid complex associated NEOs, and CM chondrites exists, we performed photometric and spectroscopic studies of these objects in the visible wavelength range. We observed 2P and 10 NEOs on Aug 2, 2011 with FORS at the VLT. Images in the R filter, used to investigate the possible presence of cometary activity around the nucleus of ...

  20. Large-Scale Mini-Magnetosphere Plasma Propulsion (M2P2) Experiments

    Science.gov (United States)

    Winglee, R. M.; Slough, J.; Ziemba, T.; Euripides, P.; Adrian, M. L.; Gallagher, D.; Craven, P.; Tomlinson, W.; Cravens, J.; Burch, J.; Whitaker, Ann F. (Technical Monitor)

    2001-01-01

    Mini-Magnetosphere Plasma Propulsion (M2P2) is an innovative plasma propulsion system that has the potential to propel spacecraft at unprecedented speeds of 50 to 80 km per second with a low-power requirement of approx. 1 kW per 100 kg of payload and approx. 1 kg of neutral gas [fuel] consumption per day of acceleration. Acceleration periods from several days to a few months are envisioned. High specific impulse and efficiency are achieved through coupling of the spacecraft to the 400 km per second solar wind through an artificial magnetosphere. The mini-magnetosphere or inflated magnetic bubble is produced by the injection of cold dense plasma into a spacecraft-generated magnetic field envelope. Magnetic bubble inflation is driven by electromagnetic processes thereby avoiding the material and deployment problems faced by mechanical solar sail designs, Here, we present the theoretical design of M2P2 as well as initial results from experimental testing of an M2P2 prototype demonstrating: 1) inflation of the dipole magnetic field geometry through the internal injection of cold plasma; and 2) deflection of and artificial solar wind by the prototype M2P2 system. In addition, we present plans for direct laboratory measurement of thrust imparted to a prototype M2P2 by an artificial solar wind during the summer of 2001.

  1. An Efficient Architecture for Information Retrieval in P2P Context Using Hypergraph

    CERN Document Server

    Ismail, Anis; Durand, Nicolas; Hajjar, Mohammad

    2011-01-01

    Peer-to-peer (P2P) Data-sharing systems now generate a significant portion of Internet traffic. P2P systems have emerged as an accepted way to share enormous volumes of data. Needs for widely distributed information systems supporting virtual organizations have given rise to a new category of P2P systems called schema-based. In such systems each peer is a database management system in itself, ex-posing its own schema. In such a setting, the main objective is the efficient search across peer databases by processing each incoming query without overly consuming bandwidth. The usability of these systems depends on successful techniques to find and retrieve data; however, efficient and effective routing of content-based queries is an emerging problem in P2P networks. This work was attended as an attempt to motivate the use of mining algorithms in the P2P context may improve the significantly the efficiency of such methods. Our proposed method based respectively on combination of clustering with hypergraphs. We use...

  2. Monitoring autophagic flux using Ref(2)P, the Drosophila p62 ortholog.

    Science.gov (United States)

    DeVorkin, Lindsay; Gorski, Sharon M

    2014-09-02

    Human p62, also known as Sequestome-1 (SQSTM1), is a multifunctional scaffold protein that contains many domains, including a Phox/Bem1P (PB1) multimerization domain, an ubiquitin-associated (UBA) domain, and a light chain 3 (LC3) recognition sequence. p62 binds ubiquitinated proteins and targets them for degradation by the proteasome. In addition, p62 directly binds LC3; this may serve as a mechanism to deliver ubiquitinated proteins for degradation by autophagy. During this process, p62 itself is degraded. The inhibition of autophagy leads to the accumulation of p62, indicating that it can be used as a marker of autophagic flux. Ref(2)P (refractory to sigma P), the Drosophila ortholog of p62, is also required for the formation of ubiquitinated protein aggregates. Ref(2)P contains a putative LC3-interacting region, and genetic inhibition of autophagy in Drosophila leads to the accumulation of Ref(2)P protein levels. Thus, like p62, Ref(2)P may serve as a marker of autophagic flux. Here we provide two procedures to examine Ref(2)P protein levels in Drosophila ovaries.

  3. Using the Reputation Score Management for Constructing Fair P2P File Sharing System

    Directory of Open Access Journals (Sweden)

    Jun Han

    2013-06-01

    Full Text Available This paper has used the reputation score management for constructing a fair P2P file sharing system, the system design principle is simple and easy to realize, and every node entering into the P2P network obtains a certain reputation score, and obtains the corresponding resources reward according to the score. This paper has described the fair sharing strategies facing node network bandwidth and TTL, and these strategies can be used independently or be combined with other reputation score managements of P2P network. These two strategies have been discussed in the specific reputation score management system of P2P network Eigen Trust, and the test results indicate that: compared with a common P2P network, the fair sharing strategies of this paper have faster file download speed and can decrease the network message communication amount during the process looking for resources. It can also be combined with another reputation management system. it is simple and easy to be realized, its main purposes are to fairly share network bandwidth and to decrease information communication volume, and it can suppress the free riding behavior to some extent.

  4. Information Exchange rather than Topology Awareness: Cooperation between P2P Overlay and Traffic Engineering

    Directory of Open Access Journals (Sweden)

    Jia Zhao

    2014-01-01

    Full Text Available Solutions to the routing strategic conflict between noncooperative P2P overlay and ISP underlay go separate ways: hyperselfishness and cooperation. Unpredictable (possibly adverse impact of the hyperselfish topology awareness, which is adopted in both overlay routing and traffic engineering, has not been sufficiently studied in the literature. Topology-related information exchange in a cooperatively efficient way should be highlighted to alleviate the cross-layer conflict. In this paper, we first illustrate the hyperselfish weakness with two dynamic noncooperative game models in which hyperselfish overlay or underlay has to accept a suboptimal profit. Then we build a synergistic cost-saving (SC game model to reduce the negative effects of noncooperation. In the SC model, through information exchange, that is, the classified path-delay metrics for P2P overlay and peer locations for underlay, P2P overlay selects proximity as well as saving traffic transit cost for underlay, and ISP underlay adjusts routing to optimize network cost as well as indicating short delay paths for P2P. Simulations based on the real and generated topologies validate cost improvement by SC model and find a proper remote threshold value to limit P2P traffic from remote area, cross-AS, or cross-ISP.

  5. Microwave Dielectric Properties of A2P2O7 (A = Ca, Sr, Ba; Mg, Zn, Mn)

    Science.gov (United States)

    Bian, Jian-jiang; Kim, Dong-Wan; Hong, Kug Sun

    2004-06-01

    Microwave dielectric properties of A2P2O7 (A = Ca, Sr, Ba, Mn, Mg, Ba) ceramic materials were investigated by a network analyzer at the frequency of 10 GHz. It was found that A2P2O7 ceramics could be sintered at relatively lower temperature below 1150°C, although the thortveitite type series, Mn2P2O7, α-Mg2P2O7 and α-Zn2P2O7 with smaller ionic radii of A cations were hard to sinter to full density. The dielectric constant of A2P2O7 is lower than 10. The Q× f value increased according to the sequence of δ-Ba2P2O7, α-Sr2P2O7 and β-Ca2P2O7 in dichromatic type series, and the sequence of Mn2P2O7, α-Mg2P2O7 and α-Zn2P2O7 in thortveitite type series, respectively. The temperature coefficient of resonant frequency τf for all samples exhibits negative value. Larger τf for α-Zn2P2O7, α-Mg2P2O7 and δ-Ba2P2O7 is mainly due to their reversible phase transformations. The microwave dielectric properties were discussed from the point view of bond valence.

  6. 共沉默miR-221-3p/222-3p表达抑制骨髓间充质干细胞增殖及促成软骨分化%Down-regulation of miR-221-3p/222-3p inhibits cell proliferation and promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells

    Institute of Scientific and Technical Information of China (English)

    闫继红; 周德山; 杨姝; 孙海梅; 曹丹丹; 张秀英; 季凤清; 郭多; 吴波; 孙婷怡

    2015-01-01

    BACKGROUND:The use of mesenchymal stem cels in the field of tissue engineering for osteoarticular injury repair is a very promising tool since these cels are readily expandable and able to differentiate into chondrocytes. Abundant evidence suggests that microRNAs play critical roles in chondrogenic differentiation of mesenchymal stem cels. OBJECTIVE:To observe the chondrogenic effect of human bone marrow mesenchymal stem cels transfected with lentiviral vectors bearing miR-221-3p/222-3p inhibition, thereby provding new strategies for cartilage injury. METHODS: miRNA microarray technology was applied to detect microRNAs expression profiles at three different stages of chondrogenic differentiation induction after transforming growth factor-β3 treatment and verified by real-time fluorescence quantitative PCR (RT-qPCR). Human bone marrow mesenchymal stem cels were infected with lentivirus bearing miR-221-3p/222-3p inhibition. After co-suppressing the expression of miR-221/222-3p, cel counting kit-8 was used to determine the cel proliferation, the differentiation of bone marrow mesenchymal stem cels towards chondrocytes was verified by type II colagen protein expression through immunohistochemistry and glycosaminoglycan accumulation was also elevated by sarranine O staining. RT-PCR was used to detect type II colagen and aggrecan mRNA expression at 21 days of chondrogenic induction. RESULTS AND CONCLUSION: The expression of miR-221-3p/222-3p was inhibited after Lv-miR221-3p/222-3p inhibition co-transfected into bone marrow mesenchymal stem cels. microRNA microarray and RT-qPCR results showed that the expression of miR-221-3p/222-3p was declined significantly at the anaphase of chondrogenic differentiation. The expression levels of chondrogenic markers, Aggrecan and type II colagen were significantly increased in the miR-221-3p/222-3p inhibition group and cel proliferation was also inhibited significantly compared with non-transduced cels or transduced with the empty

  7. Unexpected Reactivity of [(η(5) -1,2,4-tBu3 C5 H2 )Ni(η(3) -P3 )] towards Main Group Nucleophiles and by Reduction.

    Science.gov (United States)

    Mädl, Eric; Balázs, Gábor; Peresypkina, Eugenia V; Scheer, Manfred

    2016-06-27

    The reduction of [Cp'''Ni(η(3) -P3 )] (1; Cp'''=η(5) -1,2,4-tBu3 C5 H2 ) with potassium produces the complex anion [(Cp'''Ni)2 (μ,η(2:2) -P8 )](2-) (2), which contains a realgar-like P8 unit. The anionic triple-decker sandwich complex [(Cp'''Ni)2 (μ,η(3:3) -P3 )](-) (3) with a cyclo-P3 middle deck is obtained when 1 is treated with NaNH2 as a nucleophile. Na[3] can subsequently be oxidized with AgOTf to the neutral triple-decker complex [(Cp'''Ni)2 (μ,η(3:3) -P3 )] (4). In contrast, 1 reacts with LiPPh2 to give the anionic compound [(Cp'''Ni)2 (μ,η(2:2) -P6 PPh2 )](-) (5), a complex containing a bicyclic P7 fragment capped by two Cp'''Ni units. Protonation of Li[5] with HBF4 leads to the neutral complex [(Cp'''Ni)2 (μ,η(2:2) -(HP6 PPh2 )] (6). Adding LiNMe2 to 1 results in [Cp'''Ni(η(2) -P3 NMe2 )](-) (7) becoming accessible, a complex which forms as a result of nucleophilic attack at the cyclo-P3 ring of 1. The complexes K2 [2], Na[3], 4, 6, and Li[7] were fully characterized and their structures determined by single-crystal X-ray diffraction.

  8. Modelling the biogeochemical cycle of silicon in soils using the reactive transport code MIN3P

    Science.gov (United States)

    Gerard, F.; Mayer, K. U.; Hodson, M. J.; Meunier, J.

    2006-12-01

    We investigated the biogeochemical cycling of Si in an acidic brown soil covered by a coniferous forest (Douglas fir) based on a comprehensive data set and reactive transport modelling. Both published and original data enable us to make up a conceptual model on which the development of a numerical model is based. We modified the reactive transport code MIN3P, which solves thermodynamic and kinetic reactions coupled with vadose zone flow and solute transport. Simulations were performed for a one-dimensional heterogeneous soil profile and were constrained by observed data including daily soil temperature, plant transpiration, throughfall, and dissolved Si in solutions collected beneath the organic layer. Reactive transport modelling was first used to test the validity of the hypothesis that a dynamic balance between Si uptake by plants and release by weathering controls aqueous Si-concentrations. We were able to calibrate the model quite accurately by stepwise adjustment of the relevant parameters. The capability of the model to predict Si-concentrations was good. Mass balance calculations indicate that only 40% of the biogeochemical cycle of Si is controlled by weathering and that about 60% of Si-cycling is related to biological processes (i.e. Si uptake by plants and dissolution of biogenic Si). Such a large contribution of biological processes was not anticipated considering the temperate climate regime, but may be explained by the high biomass productivity of the planted coniferous species. The large contribution of passive Si-uptake by vegetation permits the conservation of seasonal concentration variations caused by temperature-induced weathering, although the modelling suggests that the latter process was of lesser importance relative to biological Si-cycling.

  9. 基于 P2P 网络的 Bitcoin 虚拟货币分析%Bitcoin Virtual Currency Analysis Based on P2P

    Institute of Scientific and Technical Information of China (English)

    石苗; 李宥谋

    2015-01-01

    Bitcoin 是一种基于 P2P 网络的匿名数字货币,论文从网络虚拟货币的发展现状出发,从技术层面详细分析Bitcoin 形成相关技术原理及其交易过程和验证机制,肯定了其历史性和革命性。同时分析 Bitcoin 存在的各方面问题,探究其在网络虚拟货币统一方面的重要借鉴意义。%Bitcoin is an anonymous digital cash based on P2P network .In this paper ,starting from the development present situation of the network virtual currency ,detailed analysis formation related technical principles and transaction process and the authentication mechanism from the technical level of Bitcoin ,affirmed its historical and revolutionary .At the same time ,aspects of problems of Bitcoin are analyzed ,its importance in the network virtual currency unification is explored .

  10. External lipid PI3P mediates entry of eukaryotic pathogen effectors into plant and animal host cells.

    Science.gov (United States)

    Kale, Shiv D; Gu, Biao; Capelluto, Daniel G S; Dou, Daolong; Feldman, Emily; Rumore, Amanda; Arredondo, Felipe D; Hanlon, Regina; Fudal, Isabelle; Rouxel, Thierry; Lawrence, Christopher B; Shan, Weixing; Tyler, Brett M

    2010-07-23

    Pathogens of plants and animals produce effector proteins that are transferred into the cytoplasm of host cells to suppress host defenses. One type of plant pathogens, oomycetes, produces effector proteins with N-terminal RXLR and dEER motifs that enable entry into host cells. We show here that effectors of another pathogen type, fungi, contain functional variants of the RXLR motif, and that the oomycete and fungal RXLR motifs enable binding to the phospholipid, phosphatidylinositol-3-phosphate (PI3P). We find that PI3P is abundant on the outer surface of plant cell plasma membranes and, furthermore, on some animal cells. All effectors could also enter human cells, suggesting that PI3P-mediated effector entry may be very widespread in plant, animal and human pathogenesis. Entry into both plant and animal cells involves lipid raft-mediated endocytosis. Blocking PI3P binding inhibited effector entry, suggesting new therapeutic avenues.

  11. Theoretical study on the two-band degenerate-gaps superconductors: Application to SrPt3P

    Science.gov (United States)

    Huang, Hai; Hou, Li-Chao; Zhao, Bin-Peng

    2016-09-01

    We study the magnetic properties of two-band degenerate-gaps superconductors with two-band isotropic Ginzburg-Landau theory. The exact solutions of upper critical field and London penetration depth are obtained, and the calculations reproduce the experimental data of the recently observed superconducting crystal SrPt3P in a broad temperature range. It directly underlies that SrPt3P is a multi-band superconductor with equal gaps in two Fermi surface sheets.

  12. The Involvement of miR-29b-3p in Arterial Calcification by Targeting Matrix Metalloproteinase-2

    Science.gov (United States)

    Jiang, Wenhong; Zhang, Zhanman; Yang, Han; Lin, Qiuning; Han, Chuangye

    2017-01-01

    Vascular calcification is a risk predictor and common pathological change in cardiovascular diseases that are associated with elastin degradation and phenotypic transformation of vascular smooth muscle cells via gelatinase matrix metalloproteinase-2 (MMP2). However, the mechanisms involved in this process remain unclear. In this study, we investigated the relationships between miR-29b-3p and MMP2, to confirm miR-29b-3p-mediated MMP2 expression at the posttranscriptional level in arterial calcification. In male Sprague Dawley rats, arterial calcification was induced by subcutaneous injection of a toxic dose of cholecalciferol. In vivo, the quantitative real-time polymerase chain reaction (qRT-PCR) showed that MMP2 expression was upregulated in calcified arterial tissues, and miR-29b-3p expression was downregulated. There was a negative correlation between MMP2 mRNA expression and miR-29b-3p levels (P = 0.0014, R2 = 0.481). Western blotting showed that MMP2 expression was significantly increased in rats treated with cholecalciferol. In vitro, overexpression of miR-29b-3p led to decreased MMP2 expression in rat vascular smooth muscle cells, while downregulation of miR-29b-3p expression led to increased MMP2 expression. Moreover, the luciferase reporter assay confirmed that MMP2 is the direct target of miR-29b-3p. Together, our results demonstrated that a role of miR-29b-3p in vascular calcification involves targeting MMP2. PMID:28164126

  13. The Involvement of miR-29b-3p in Arterial Calcification by Targeting Matrix Metalloproteinase-2

    Directory of Open Access Journals (Sweden)

    Wenhong Jiang

    2017-01-01

    Full Text Available Vascular calcification is a risk predictor and common pathological change in cardiovascular diseases that are associated with elastin degradation and phenotypic transformation of vascular smooth muscle cells via gelatinase matrix metalloproteinase-2 (MMP2. However, the mechanisms involved in this process remain unclear. In this study, we investigated the relationships between miR-29b-3p and MMP2, to confirm miR-29b-3p-mediated MMP2 expression at the posttranscriptional level in arterial calcification. In male Sprague Dawley rats, arterial calcification was induced by subcutaneous injection of a toxic dose of cholecalciferol. In vivo, the quantitative real-time polymerase chain reaction (qRT-PCR showed that MMP2 expression was upregulated in calcified arterial tissues, and miR-29b-3p expression was downregulated. There was a negative correlation between MMP2 mRNA expression and miR-29b-3p levels (P=0.0014, R2=0.481. Western blotting showed that MMP2 expression was significantly increased in rats treated with cholecalciferol. In vitro, overexpression of miR-29b-3p led to decreased MMP2 expression in rat vascular smooth muscle cells, while downregulation of miR-29b-3p expression led to increased MMP2 expression. Moreover, the luciferase reporter assay confirmed that MMP2 is the direct target of miR-29b-3p. Together, our results demonstrated that a role of miR-29b-3p in vascular calcification involves targeting MMP2.

  14. P2P Lending Risk Contagion Analysis Based on a Complex Network Model

    Directory of Open Access Journals (Sweden)

    Qi Wei

    2016-01-01

    Full Text Available This paper analyzes two major channels of P2P lending risk contagion in China—direct risk contagion between platforms and indirect risk contagion with other financial organizations as the contagion medium. Based on this analysis, the current study constructs a complex network model of P2P lending risk contagion in China and performs dynamics analogue simulations in order to analyze general characteristics of direct risk contagion among China’s online P2P lending platforms. The assumed conditions are that other financial organizations act as the contagion medium, with variations in the risk contagion characteristics set under the condition of significant information asymmetry in Internet lending. It is indicated that, compared to direct risk contagion among platforms, both financial organizations acting as the contagion medium and information asymmetry magnify the effect of risk contagion. It is also found that the superposition of media effects and information asymmetry is more likely to magnify the risk contagion effect.

  15. Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100

    Directory of Open Access Journals (Sweden)

    Víctor Toledano

    2015-01-01

    Full Text Available We show that galactomannan, a polysaccharide consisting of a mannose backbone with galactose side groups present on the cell wall of several fungi, induces a reprogramming of the inflammatory response in human macrophages through dectin-1 receptor. The nuclear factor kappa-light-chain-enhancer of activated B cells 2 (NFκB2/p100 was overexpressed after galactomannan challenge. Knocking down NFκB2/p100 using small interfering RNA (siRNA indicated that NFκB2/p100 expression is a crucial factor in the progression of the galactomannan-induced refractoriness. The data presented in this study could be used as a modulator of inflammatory response in clinical situations where refractory state is required.

  16. 11th International Conference on P2P, Parallel, Grid, Cloud and Internet Computing

    CERN Document Server

    Barolli, Leonard; Amato, Flora

    2017-01-01

    P2P, Grid, Cloud and Internet computing technologies have been very fast established as breakthrough paradigms for solving complex problems by enabling aggregation and sharing of an increasing variety of distributed computational resources at large scale. The aim of this volume is to provide latest research findings, innovative research results, methods and development techniques from both theoretical and practical perspectives related to P2P, Grid, Cloud and Internet computing as well as to reveal synergies among such large scale computing paradigms. This proceedings volume presents the results of the 11th International Conference on P2P, Parallel, Grid, Cloud And Internet Computing (3PGCIC-2016), held November 5-7, 2016, at Soonchunhyang University, Asan, Korea.

  17. Atypical features of a Ure2p glutathione transferase from Phanerochaete chrysosporium.

    Science.gov (United States)

    Thuillier, Anne; Roret, Thomas; Favier, Frédérique; Gelhaye, Eric; Jacquot, Jean-Pierre; Didierjean, Claude; Morel-Rouhier, Mélanie

    2013-07-11

    Glutathione transferases (GSTs) are known to transfer glutathione onto small hydrophobic molecules in detoxification reactions. The GST Ure2pB1 from Phanerochaete chrysosporium exhibits atypical features, i.e. the presence of two glutathione binding sites and a high affinity towards oxidized glutathione. Moreover, PcUre2pB1 is able to efficiently deglutathionylate GS-phenacylacetophenone. Catalysis is not mediated by the cysteines of the protein but rather by the one of glutathione and an asparagine residue plays a key role in glutathione stabilization. Interestingly PcUre2pB1 interacts in vitro with a GST of the omega class. These properties are discussed in the physiological context of wood degrading fungi.

  18. High-pressure elastic properties of cubic Ir2P from ab initio calculations

    Science.gov (United States)

    Sun, Xiao-Wei; Bioud, Nadhira; Fu, Zhi-Jian; Wei, Xiao-Ping; Song, Ting; Li, Zheng-Wei

    2016-10-01

    A study of the high-pressure elastic properties of new synthetic Ir2P in the anti-fluorite structure is conducted using ab initio calculations based on density functional theory. The elastic constants C11, C12 and C44 for the cubic Ir2P are obtained by the stress-strain method and the elastic stability calculations under pressure indicate that it is stable at least 100 GPa. Additionally, the electronic density of states, the aggregate elastic moduli, that is bulk modulus, shear modulus, and Young's modulus along with the Debye temperature, Poisson's ratio, and elastic anisotropy factor are all successfully obtained. Moreover, the pressure dependence of the longitudinal and shear wave velocities in three different directions [100], [110], and [111] for Ir2P are also predicted for the first time.

  19. Analytical Modelling of Localized P2P Streaming Systems under NAT Consideration

    Directory of Open Access Journals (Sweden)

    Mohammad Z. M. Masoud

    2013-06-01

    Full Text Available NAT has been design to work with Internet client-server structure. The emerged of Peer-to-Peer (P2Pnetworks and applications revealed the incompatibility between P2P applications and NAT. Many methodshas been developed and implemented to solve connectivity between peers behind NAT devices.Nevertheless, various NATing types can’t communicate with one another. In this work, we are going tostudy the impact of NAT types on the start-up delay time of peers in P2P streaming systems. We willdemonstrate the ability of NATing to expel peers in P2P live streaming systems. A new neighbour selectingalgorithm will be proposed. This algorithm will utilize NAT-types configurations as a parameter. We haveutilized NS2 simulator to show the performance of the new algorithm in increasing the connectivity,reducing the number of expelled peers and implementing of locality.

  20. A New Caching Technique to Support Conjunctive Queries in P2P DHT

    Science.gov (United States)

    Kobatake, Koji; Tagashira, Shigeaki; Fujita, Satoshi

    P2P DHT (Peer-to-Peer Distributed Hash Table) is one of typical techniques for realizing an efficient management of shared resources distributed over a network and a keyword search over such networks in a fully distributed manner. In this paper, we propose a new method for supporting conjunctive queries in P2P DHT. The basic idea of the proposed technique is to share a global information on past trials by conducting a local caching of search results for conjunctive queries and by registering the fact to the global DHT. Such a result caching is expected to significantly reduce the amount of transmitted data compared with conventional schemes. The effect of the proposed method is experimentally evaluated by simulation. The result of experiments indicates that by using the proposed method, the amount of returned data is reduced by 60% compared with conventional P2P DHT which does not support conjunctive queries.

  1. Spin correlations in (Mn,Fe2(P,Si magnetocaloric compounds above Curie temperature

    Directory of Open Access Journals (Sweden)

    X.F. Miao

    2016-06-01

    Full Text Available The longitudinal-field muon-spin relaxation (LF-μSR technique was employed to study the spin correlations in (Mn,Fe2(P,Si compounds above the ferromagnetic transition temperature (TC. The (Mn,Fe2(P,Si compound under study is found to show itinerant magnetism. The standard deviation of the magnetic field distribution of electronic origin increases with a decrease in temperature, which is attributed to the development of spin correlations. The anomalously low magnetic fluctuation rate is suggested to be another signature of the spin correlations. The development of pronounced magnetic fluctuations is in agreement with the observed deviation of the paramagnetic susceptibility from Curie–Weiss behavior. Our study sheds light on the magneto-elastic transition and the mixed magnetism in (Mn,Fe2(P,Si compounds.

  2. The N2-P3 complex of the evoked potential and human performance

    Science.gov (United States)

    Odonnell, Brian F.; Cohen, Ronald A.

    1988-01-01

    The N2-P3 complex and other endogenous components of human evoked potential provide a set of tools for the investigation of human perceptual and cognitive processes. These multidimensional measures of central nervous system bioelectrical activity respond to a variety of environmental and internal factors which have been experimentally characterized. Their application to the analysis of human performance in naturalistic task environments is just beginning. Converging evidence suggests that the N2-P3 complex reflects processes of stimulus evaluation, perceptual resource allocation, and decision making that proceed in parallel, rather than in series, with response generation. Utilization of these EP components may provide insights into the central nervous system mechanisms modulating task performance unavailable from behavioral measures alone. The sensitivity of the N2-P3 complex to neuropathology, psychopathology, and pharmacological manipulation suggests that these components might provide sensitive markers for the effects of environmental stressors on the human central nervous system.

  3. Public Key Cryptosystem Approach for P2P Botnet Detection and Prevention

    Directory of Open Access Journals (Sweden)

    Anas Aliyu Usman

    2015-04-01

    Full Text Available Distributed (P2P botnets have as of late been received by botmasters for their versatility against take-down endeavors. Other than being harder to bring down, p2p botnets tend to be stealthier in the way they perform vindictive exercises, making current discovery approaches ineffectual. In this paper, we simulate our proposal by detecting a gray hole attack in an Ad Hoc network using NS2.The detected malicious node is listed in a black hole list and notices all other nodes in the network to stop communicating with them. Our botnet location framework has been equipped for identifying stealthy P2P botnets (Gray Hole nodes and can reduce packet loss caused by malicious nodes and have a better packet delivery ratio (PDR within less period of time.

  4. The 2p-2h electromagnetic response in the quasielastic peak and beyond

    Energy Technology Data Exchange (ETDEWEB)

    De Pace, A. E-mail: depace@to.infn.it; Nardi, M.; Alberico, W.M.; Donnelly, T.W.; Molinari, A

    2003-10-20

    The contribution to the nuclear transverse response function R{sub T} arising from two particle-two hole (2p-2h) states excited through the action of electromagnetic meson exchange currents (MEC) is computed in a fully relativistic framework. The MEC considered are those carried by the pion and by {delta} degrees of freedom, the latter being viewed as a virtual nucleonic resonance. The calculation is performed in the relativistic Fermi gas model in which Lorentz covariance can be maintained. All 2p-2h many-body diagrams containing two pionic lines that contribute to R{sub T} are taken into account and the relative impact of the various components of the MEC on R{sub T} is addressed. The non-relativistic limit of the MEC contributions is also discussed and compared with the relativistic results to explore the role played by relativity in obtaining the 2p-2h nuclear response.

  5. On the Design and Implementation of Structured P2P VPNs

    CERN Document Server

    Wolinsky, David Isaac; Lee, Kyungyong; Liu, Yonggang; Xu, Jiangyan; Boykin, P Oscar; Figueiredo, Renato

    2010-01-01

    Centralized Virtual Private Networks (VPNs) when used in distributed systems have performance constraints as all traffic must traverse through a central server. In recent years, there has been a paradigm shift towards the use of P2P in VPNs to alleviate pressure placed upon the central server by allowing participants to communicate directly with each other, relegating the server to handling session management and supporting NAT traversal using relays when necessary. Another, less common, approach uses unstructured P2P systems to remove all centralization from the VPN. These approaches currently lack the depth in security options provided by other VPN solutions, and their scalability constraints have not been well studied. In this paper, we propose and implement a novel VPN architecture, which uses a structured P2P system for peer discovery, session management, NAT traversal, and autonomic relay selection and a central server as a partially-automated public key infrastructure (PKI) via a user-friendly web inte...

  6. Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis.

    Science.gov (United States)

    Dostál, Jiří; Pecina, Adam; Hrušková-Heidingsfeldová, Olga; Marečková, Lucie; Pichová, Iva; Řezáčová, Pavlina; Lepšík, Martin; Brynda, Jiří

    2015-12-01

    The virulence of the Candida pathogens is enhanced by the production of secreted aspartic proteases, which therefore represent possible targets for drug design. Here, the crystal structure of the secreted aspartic protease Sapp2p from Candida parapsilosis was determined. Sapp2p was isolated from its natural source and crystallized in complex with pepstatin A, a classical aspartic protease inhibitor. The atomic resolution of 0.83 Å allowed the protonation states of the active-site residues to be inferred. A detailed comparison of the structure of Sapp2p with the structure of Sapp1p, the most abundant C. parapsilosis secreted aspartic protease, was performed. The analysis, which included advanced quantum-chemical interaction-energy calculations, uncovered molecular details that allowed the experimentally observed equipotent inhibition of both isoenzymes by pepstatin A to be rationalized.

  7. A P2P Botnet Virus Detection System Based on Data-Mining Algorithms

    Directory of Open Access Journals (Sweden)

    Wernhuar Tarng

    2012-11-01

    Full Text Available A P2P botnet virus detection system based on data-mining algorithms is proposed in this study to detect theinfected computers quickly using Bayes Classifier and Neural Network (NN Classifier. The system candetect P2P botnet viruses in the early stage of infection and report to network managers to avoid furtherinfection. The system adopts real-time flow identification techniques to detect traffic flows produced by P2Papplication programs and botnet viruses by comparing with the known flow patterns in the database. Aftertrained by adjusting the system parameters using test samples, the experimental results show that theaccuracy of Bayes Classifier is 95.78% and that of NN Classifier is 98.71% in detecting P2P botnet virusesand suspected flows to achieve the goal of infection control in a short time.

  8. Kinetic-arrest-induced phase coexistence and metastability in (Mn,Fe ) 2(P ,Si )

    Science.gov (United States)

    Miao, X. F.; Mitsui, Y.; Dugulan, A. Iulian; Caron, L.; Thang, N. V.; Manuel, P.; Koyama, K.; Takahashi, K.; van Dijk, N. H.; Brück, E.

    2016-09-01

    Neutron diffraction, Mössbauer spectroscopy, magnetometry, and in-field x-ray diffraction are employed to investigate the magnetoelastic phase transition in hexagonal (Mn,Fe ) 2(P ,Si ) compounds. (Mn,Fe ) 2(P ,Si ) compounds undergo for certain compositions a second-order paramagnetic (PM) to a spin-density-wave (SDW) phase transition before further transforming into a ferromagnetic (FM) phase via a first-order phase transition. The SDW-FM transition can be kinetically arrested, causing the coexistence of FM and untransformed SDW phases at low temperatures. Our in-field x-ray diffraction and magnetic relaxation measurements clearly reveal the metastability of the untransformed SDW phase. This unusual magnetic configuration originates from the strong magnetoelastic coupling and the mixed magnetism in hexagonal (Mn,Fe ) 2(P ,Si ) compounds.

  9. P2PRPIPS: A P2P and Reverse Proxy Based Web Intrusion Protection System

    Directory of Open Access Journals (Sweden)

    Qian He

    2013-03-01

    Full Text Available In order to protect web sites with various program languages and high throughput efficiently, a web Intrusion Protection System (IPS based on P2P and reverse proxy architecture was designed and implemented. The P2P based web intrusion protection system has multi web firewall nodes and nodes with same program cooperate with each other under P2P architecture. Some nodes work as net flow allocator and some work as detector and they can convert to each other according to the requirements dynamically. The WAF program has the characteristics of session keeping and load balancing and it can detect messages by using expert library and many plug-in components. The technology of reverse proxy is used for response the web request. Experiments show that the system can effectively prevent attacks form application layer. It is proved more efficient and stable than single node.

  10. Supporting K nearest neighbors query on high-dimensional data in P2P systems

    Institute of Scientific and Technical Information of China (English)

    Mei LI; Wang-Chien LEE; Anand SIVASUBRAMANIAM; Jizhong ZHAO

    2008-01-01

    Peer-to-peer systems have been widely used for sharing and exchanging data and resources among numerous computer nodes.Various data objects identifiable with high dimensional feature vectors,such as text,images,genome sequences,are starting to leverage P2P technology.Most of the existing works have been focusing on queries on data objects with one or few attributes and thus are not applicable on high dimensional data objects.In this study,we investigate K nearest neighbors query (KNN)on high dimensional data objects in P2P systems.Efficient query algorithm and solutions that address various technical challenges raised by high dimensionality,such as search space resolution and incremental search space refinement,are proposed.An extensive simulation using both synthetic and real data sets demonstrates that our proposal efficiently supports KNN query on high dimensional data in P2P systems.

  11. Relay Ability Estimation and Topology Control Using Multidimensional Context Parameters for Mobile P2P Multicast

    Directory of Open Access Journals (Sweden)

    Kubo Hiroyuki

    2011-01-01

    Full Text Available We focus on mobile P2P multicast, in which mobile end nodes not only act as receivers but also relay the received stream forward to others. In mobile P2P multicast, negative effects caused by the change of available bandwidth and the disconnection of mobile nodes are propagated to the downstream nodes. To solve this problem, we developed a novel node-allocation framework using the multidimensional context parameters of each mobile node, which include available bandwidth, disconnection rate, and the remaining battery capacity. Considering the significance of each parameter, our method integrates these parameters into a single parameter called relay ability. Taking the relay ability into account, each node is allocated to the multicast topology to minimize the negative effects mentioned above. To test our method, we applied our framework to conventional P2P multicast topology and show the results from comparative evaluations through computer simulation.

  12. Distributed Algorithms for Improving Search Efficiency in P2P Overlays

    Directory of Open Access Journals (Sweden)

    Chittaranjan Hota

    2012-04-01

    Full Text Available Peer-to-peer (P2P overlay is a distributed application architecture in which peers share their resources. Peers are equally privileged, equipotent participants in the application. Several algorithms for enhancing P2P file searching have been proposed in the literature. In this paper, we have proposed a unique approach of reducing the P2P search complexity and improving search efficiency by using distributed algorithms. In our approach a peer mounts other popular peer’s files and also replicates other popular files or critical files identified using a threshold value. Once a file is mounted, file access requests can be serviced by transparently retrieving the file and sending it to the requesting peer. Replication used in this work improves the file retrieval time by allowing parallel transfer. We have shown the performance analysis of our proposed approach which shows improvement in the search efficiency.

  13. Ginkgolide B Inhibits Human Bladder Cancer Cell Migration and Invasion Through MicroRNA-223-3p

    Directory of Open Access Journals (Sweden)

    Yi Zhi

    2016-10-01

    Full Text Available Background/Aims: Ginkgolide B (GB is currently used as an anticancer drug for treatment of some malignant cancers. However, whether it may have therapeutic effects on bladder cancer remains unknown. Here, we studied the effects of GB on bladder cancer cells. Methods: Bladder cells were treated with different doses of GB, and the effects on ZEB1 and microRNA-223-3p (miR-223-3p were analyzed by RT-qPCR and/or Western blot. Prediction of a regulatory relationship between miR-93 and 3'-UTR of Beclin-1 mRNA was performed by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Results: We found that GB dose-dependently decreased ZEB1 protein, but not mRNA, in bladder cancer cells, resulting in suppression of cell invasion. Moreover, in bladder cancer cells, GB dose-dependently decreased the levels of miR-223-3p, which suppressed the protein translation of ZEB1 through binding to 3'-UTR of ZEB1 mRNA. Overexpression of miR-223-3p decreased ZEB1 protein, while depletion of miR-223-3p increased ZEB1 protein in bladder cancer cells. Conclusion: GB inhibits bladder cancer cell invasiveness through suppressing ZEB1 protein translation via upregulating miR-223-3p.

  14. MiR-525-3p enhances the migration and invasion of liver cancer cells by downregulating ZNF395.

    Directory of Open Access Journals (Sweden)

    Fei Pang

    Full Text Available Liver cancer is one of leading causes of cancer-related deaths. A deeper mechanistic understanding of liver cancer could lead to the development of more effective therapeutic strategies. In our previous work, we screened 646 miRNAs and identified 11 that regulate liver cancer cell migration. The current study shows that miR-525-3p is frequently up-regulated in liver cancer tissues, and enhanced expression of miR-525-3p can promote liver cancer cell migration and invasion. Zinc finger protein 395 (ZNF395 is the direct functional target gene for miR-525-3p, and it is frequently down-regulated in liver cancer tissues. High expression of ZNF395 can significantly inhibit while knockdown of ZNF395 expression can markedly enhance the migration and invasion of liver cancer cells, suggesting that ZNF395 suppresses metastasis in liver cancer. Down-regulation of ZNF395 can mediate miR-525-3p induced liver cancer cell migration and invasion. In conclusion, miR-525-3p promotes liver cancer cell migration and invasion by directly targeting ZNF395, and the fact that miR-525-3p and ZNF395 both play important roles in liver cancer progression makes them potential therapeutic targets.

  15. Bonding and site preferences in the new quasi-binary Zr{sub 2.7}Hf{sub 11.3}P{sub 9}

    Energy Technology Data Exchange (ETDEWEB)

    Kleinke, H.; Franzen, H.F. [Ames Lab., IA (United States)

    1998-03-01

    The new quasi-binary Zr{sub 2.7}Hf{sub 11.3}P{sub 9} was synthesized by arc-melting of Zr, Hf, Co, and HfP in a ratio corresponding to the initial composition Zr{sub 2.25}Hf{sub 6.75}Co{sub 2}P{sub 4}. Zr{sub 2.7}Hf{sub 11.3}P{sub 9} crystallizes in the Zr{sub 14}P{sub 9} structure type, which is unknown in the binary Hf/P system. the ideal orthorhomibic lattice dimensions (space group Pnnm (No. 58), Z = 4) were refined to a = 16.640(7) {angstrom}, b = 27.40(2) {angstrom}, c = 3.619(1) {angstrom}, V = 1650(2) {angstrom}{sup 3}. The structure consists of three-dimensional condensed one-, two-, and three-capped trigonal (Zr, Hf){sub 6}P prisms, occurring with numerous short M-M bonds (M = Zr, Hf). Each of the 15 metal sites is statistically occupied by a mixture of Zr and Hf, which varies significantly from site to site. The Hf/Zr ratio in a given site depends on the M-M and M-P interactions. The systematic increase of this ratio with increasing total bond order, as evaluated via Mulliken overlap populations and Pauling bond orders, can be understood based on the trend that Hf forms stronger M-M and M-P bonds than Zr. As expected for a metal-rich phosphide, band structure calculations for the hypothetical Hf{sub 14}P{sub 9} carried out with the extended Hueckel approximation result in a significant density of states at the Fermi level.

  16. Efficient content-based P2P image retrieval using peer content descriptions

    Science.gov (United States)

    Muller, Wolfgang T.; Eisenhardt, Martin; Henrich, Andreas

    2003-12-01

    Peer-to-peer (P2P) networks are overlay networks that connect independent computers (also called nodes or peers). In contrast to client/server solutions, all nodes offer and request services from other peers in a P2P network. P2P networks are very attractive in that they harness the computing power of many common desktop machines and necessitate little administrative overhead. While the resulting computing power is impressive, efficiently looking up data still is the major challenge in P2P networks. Current work comprises fast lookup of one-dimensional values (Distributed Hash Tables, DHT) and retrieval of texts using few keywords. However, the lookup of multimedia data in P2P networks is still attacked by very few groups. In this paper, we present experiments with efficient Content Based Image Retrieval in a P2P environment, thus a P2P-CBIR system. The challenge in such systems is to limit the number of messages sent, and to maximize the usefulness of each peer contacted in the query process. We achieve this by distributing peer data summaries over the network. Obviously, the data summaries have to be compact in order to limit the communication overhead. We propose an CBIR scheme based on a compact peer data summary. This peer data summary relies on cluster frequencies. To obtain the compact representation of a peer's collection, a global clustering of the data is efficiently calculated in a distributed manner. After that, each peer publishes how many of its images fall into each cluster. These cluster frequencies are then used by the querying peer to contact only those peers that have the largest number of images present in one cluster given by the query. In our paper we further detail the various challenges that have to be met by the designers of such a P2P-CBIR, and we present experiments with varying degree of data replication (duplicates of images), as well as quality of clustering within the network.

  17. Integrating XQuery and P2P in MonetDB/XQuery*

    OpenAIRE

    Zhang, Ying; Boncz, Peter; Arenas, M; Hidders, J.

    2007-01-01

    MonetDB/XQuery* is a fully functional publicly available XML DBMS that has been extended with distributed and P2P data management functionality. Our (minimal) XQuery language extension XRPC adds the concept of RPC to XQuery, and exploits the set-at-a-time database processing model to optimize the networking cost through a technique called Bulk RPC. We describe our approach to include the services offered by diverse P2P network structures (such as DHTs), in a way that avoids any further intrus...

  18. Cross-layer P2P traffic engineering in content-based networks.

    OpenAIRE

    Zhang, Xu

    2015-01-01

    Peer-to-peer (P2P) content sharing applications account for a significant fraction of the traffic volumes and is expected to increase [1]. Data is distributed to a large population of end client peers from end source peers in P2P systems, without the need for big investments to deploy servers. The costs of the content distribution are thus shared among end users and Internet service providers (ISPs). Consequently, negative impacts, such as increased inter-ISP traffic in particular, have becom...

  19. Photoionization of Ge13+ in the Inner-Shell 2p Excitation Energy Region

    Institute of Scientific and Technical Information of China (English)

    LIANG Gui-Yun; ZHAO Gang

    2006-01-01

    The photoionization cross sections of the levels belonging to the ground configuration [Ne]3s23p63d of Ge13+ are investigated using the fully relativistic R-matrix method in the 2p-3d excitation region.The photoionization cross section is dominated by 2p-3d resonances.The detailed resonance structures are described and analysed with the resonance positions,widths and oscillator strengths to be determined.Good agreement is obtained between the length and velocity forms of the resonallce oscillator strengths.The relative difference is less than 8%.

  20. Si/SiO2/p-Si结构的电学特性

    Institute of Scientific and Technical Information of China (English)

    马自军; 马书懿

    2008-01-01

    用射频磁控溅射法在p-Si衬底上制备了Si/SiO2薄膜,利用Au/(Si/SiO2)/p-Si结构的I-V特性曲线对其电学特性进行了分析.结果表明,样品具有很好的整流作用,起整流作用的势垒存在于(Si/SiO2)/p-Si界面附近.

  1. A Hybrid P2P Approach to Service Discovery in the Cloud

    Directory of Open Access Journals (Sweden)

    Nor Aniza Abdullah

    2011-02-01

    Full Text Available Highly scalable techniques for service discovery are key to the efficient use of Cloud resources, since the Cloud computing appears to be part of the mainstream computing in a few years. We embarked on a preliminary study on Cloud service discovery by adopting an unstructured P2P paradigm. We developed an efficient mechanism for routing of service requests by coupling a number of components: one-hop replication, semanticaware message routing, topology reorganization, and supernodes. A number of experiments were carried out that demonstrated the expected performance of the proposed P2P search scheme.

  2. 2S-2P Lamb shift and 2S hyperfine splitting in muonic hydrogen

    CERN Document Server

    Antognini, Aldo; Biraben, François; Indelicato, Paul; Nez, François; Pohl, Randolf

    2012-01-01

    The 7 standard deviations between the proton rms charge radius from muonic hydrogen and the CODATA-10 value from hydrogen spectroscopy and electron-scattering has caused considerable discussions. Here, we review the theory of the 2S-2P Lamb shift and 2S hyperfine splitting in muonic hydrogen combining the published contributions and theoretical approaches. The prediction of these quantities is necessary for the determination of both proton charge and Zemach radii from the two 2S-2P transition frequencies measured in muonic hydrogen.

  3. Enabling Co-located Learning over Mobile Ad Hoc P2P with LightPeers

    DEFF Research Database (Denmark)

    Christensen, Bent Guldbjerg; Kristensen, Mads Darø; Hansen, Frank Allan

    2008-01-01

    This paper presents LightPeers – a new mobile P2P framework specifically tailored for use in a nomadic learning environment. A set of key requirements for the framework is identified based on nomadic learning, and these requirements are used as outset for designing and implementing the architecture...... and protocols. The main contributions of the LightPeers framework are: a mobile P2P framework including a specialized robust messaging protocol resilient to changes in the network topology, an developers API, and a suite of LightPeers applications supporting nomadic learning prototyping key features of LightPeers....

  4. Are 2P/Encke, the Taurid complex NEOs and CM chondrites related?

    Science.gov (United States)

    Tubiana, C.; Snodgrass, C.; Michelsen, R.; Haack, H.; Fitzsimmons, A.; Williams, I.; Boehnhardt, H.

    2013-09-01

    Comet 2P/Encke is a short-period comet that was discovered in 1786 and has been extensively observed and studied for more than 200 years. It has an orbital period of 3.3 years and its orbit is dynamically decoupled from Jupiter's control due to gravitational interaction with terrestrial planets [6]. It is the only comet known on such an orbit, making it unique. Capture from the outer solar system onto its current orbit is very unlikely and even a continuous smooth dynamical evolution has a low probability as this requires a continuous period when it is dormant in order to avoid the volatiles from the nucleus becoming exhausted and making the current observed activity impossible. An origin in the asteroid belt is a possibility especially in view of the recently discovered main belt comets. The nucleus of 2P/Encke is dark (geometric albedo of 0.047 ± 0.023 [3]), has an effective radius of 2.4 ± 0.3 km [3] and it has polarimetric properties that are unique compared to other measured types of solar system objects, such as asteroids, TNOs, cometary dust, Centaurs [2]. The colors of 2P/Encke's nucleus are typical for comets, but no spectra of the nucleus in the visible wavelength range exist so far. The Taurid meteoroid stream has long been linked with 2P/Encke, but the activity of the comet is not strong enough to explain the number of observed meteors. It has been suggested that the meteoroid stream was caused by the break up of a larger parent body, which left comet 2P/Encke and other various small bodies along with a stream of dust. Various small near-Earth objects (NEOs) have been discovered with orbits that can be linked with 2P/Encke and the Taurid meteoroid stream [1]. Though many of the associations are spurious due to the low inclination of 2P/Encke's orbit, many NEO's have evolved in a similar way to 2P/Encke overa period of 5000 years [8] suggesting some relationship. In addition to dynamical properties, common taxonomic properties can also provide an

  5. Quantum electrodynamics corrections to the 2P fine splitting in Li.

    Science.gov (United States)

    Puchalski, Mariusz; Pachucki, Krzysztof

    2014-08-15

    We consider quantum electrodynamics (QED) corrections to the fine splitting E(2P_{3/2})-E(2P_{1/2}) in the Li atom. We derive complete formulas for the mα^{6} and mα^{7}lnα contributions and calculate them numerically using highly optimized, explicitly correlated basis functions. The obtained results are in agreement with the most recent measurement, helping to resolve discrepancies between former ones and lay the foundation for the investigation of QED effects in light, many-electron atoms.

  6. A Participation-Based Trust Model for Mobile P2P Networks

    Directory of Open Access Journals (Sweden)

    Kai Liu

    2014-07-01

    Full Text Available Mobile peer-to-peer (P2P networks have become increasingly popular, but the attacks and selfness of peers make it vulnerable. In this paper, a trust model based on participation is proposed for mobile P2P networks (called ParticipationTrust. It could deal with the egoistic and malicious behaviors. And the level of peer’s participation could be calculated through the transaction participation and recommendation participation. A set of experiments show that the model is rational and effective

  7. Cluster based hierarchical resource searching model in P2P network

    Institute of Scientific and Technical Information of China (English)

    Yang Ruijuan; Liu Jian; Tian Jingwen

    2007-01-01

    For the problem of large network load generated by the Gnutella resource-searching model in Peer to Peer (P2P) network, a improved model to decrease the network expense is proposed, which establishes a duster in P2P network,auto-organizes logical layers, and applies a hybrid mechanism of directional searching and flooding. The performance analysis and simulation results show that the proposed hierarchical searching model has availably reduced the generated message load and that its searching-response time performance is as fairly good as that of the Gnutella model.

  8. Chemical preparation, kinetics of thermal behavior and infrared studies of Pb3(P3O92.3H2O and Cd3(P3O92.14H2O

    Directory of Open Access Journals (Sweden)

    Said Belaaouad

    2013-05-01

    Full Text Available Chemical preparation, thermal behavior, kinetic and IR studies are given for the cyclotriphosphates Pb3(P3O92.3H2O and Cd3(P3O92.14H2O. The later cyclotriphosphates have never been studied except their crystallographic characterization and are stable in the conditions of temperature and pressure of our laboratory until 343K. The final products of the dehydration and calcination of Pb3(P3O92.3H2O and Cd3(P3O92.14H2O, under atmospheric pressure, are respectively their long chain polyphosphates, [Pb(PO32]∞ and β[Cd(PO32]∞. The intermediate product of the dehydration of Cd3(P3O92.14H2O, under atmospheric pressure, is its long chain polyphosphate form α, α[Cd(PO32]. [Pb(PO32]∞ and β[Cd(PO32]∞ are stable until their melting points at respectively 946K and 1153K. Two different methods, Ozawa and KAS have been selected in order to study the kinetics of thermal behavior of the cyclotriphosphates Pb3(P3O92.3H2O and Cd3(P3O92.14H2O for the first time. The kinetic and thermodynamic features of the dehydration, of the cited cyclotriphosphates, were determined and discussed on the basis of their crystalline structure. [Pb(PO32]∞, α[Cd(PO32] and β[Cd(PO32] have many applications in industry such as corrosion inhibitors.

  9. Relationship between miRNA-338-3p expression and progression and prognosis of human colorectal carcinoma

    Institute of Scientific and Technical Information of China (English)

    Sun Kai; Su Guiyuan; Deng Haijun; Dong Jingqing; Lei Shangtong; Li Guoxin

    2014-01-01

    Background miR-338-3p is a recently discovered miRNA and is involved in cell differentiation.However,few data are yet available on the aberrant expression of miR-338-3p in human colorectal carcinoma (CRC).This work aimed to investigate the relationship between miR-338-3p expression pattern and clinicopathological features of human CRC and the possible regulative mechanisms.Methods The 40 CRC,adjacent nontumorous tissues and 2 human CRC-derived cell lines (SW-480 and SW-620) were collected,respectively,and the total RNA and protein were isolated routinely.The miR-338-3p expression pattern was detected by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Northern blotting.Smoothened (SMO,possible target of miR-338-3p) mRNA and corresponding protein expression pattern were detected by semiquantitative RT-PCR and Western blotting.miR-338-3p expression patterns were compared between nontumor mucosa and CRC samples,graded by progression-related factors.Disease outcome was calculated by Kaplan-Meier survival analysis to determine whether miR-338-3p was related to disease-free survival (DFS) and overall survival (OS) of patients.Moreover,SMO 3'-UTR fragment was PCR amplified from genome DNA of human colon and inserted into a luciferase reporter plasmid.The luciferase reporter plasmid construct was then transfected into CRC cells together with pre-miR-338-3p or anti-miR-338-3p and the luciferase activity in the transfected cells was detected.Results The expression of miR-338-3p was significantly downregulated in CRCs than those in the adjacent nontumorous tissues,and the value was negatively related to advanced TNM stage and local invasion (P <0.01).Furthermore,miR338-3p value was decreased markedly in SW-620 cell line relative to SW-480 (P <0.01).Low expression of miR-338-3p was associated with unfavorable outcome in DFS but not in OS independent of clinical covariates.Moreover,RT-PCR and Western blotting analysis demonstrated that there was no

  10. Reactions of N+ (3P) ions with H2 and HD molecules at low temperatures

    Science.gov (United States)

    Grozdanov, Tasko P.; McCarroll, Ronald; Roueff, Evelyne

    2016-05-01

    formation of ND+. The calculated value is consistent with the available experimental data. Conclusions: The present results allow for the determination of reaction rate coefficients for any given distribution of specific fine structure and rotational state populations of the reactants. In interstellar conditions, where N+ is in its 3P0 state and para- and ortho-H2 respectively in J = 0 and J = 1. Our results enable a study of the influence of the ortho/para evolution of molecular hydrogen on the formation of nitrogen compounds.

  11. Research of the lime rich portions of the CaO-SiO2-P2O5 system

    Directory of Open Access Journals (Sweden)

    Puertas, F.

    1995-03-01

    Full Text Available A detailed study has been carried out to explain the influence of Pp^ in silicate phases and in industrial raw meals upon their mineralogical compositions. Some compositions in the lime rich portion of the CaO-SiO2-P2O5 system are studied using laboratory reactants. The results showed that the compositions lying in the range of CaO = 69,5-70,1; P2O5= 16,2-19,5; SiO2 = 11,1-13,6 (wt% give, at 1000 °C, the mineralogical composition (HAp, β-C2S, C5PS which can develop a high strength in chemically bonded ceramics. At high temperature (1400 ºC these compositions give a large amount of silicocarnotite (C5PS and 2C2.C3P phase. A selected composition of the industrial raw meal with added phosphate: CaO = 62,66; SiO2 -= 17,33; Al2O3 = 4,88; Fe2O3 = 4,29; P2O5 = 8,73; MgO = 2,09 (wt% forms at 1350 ºC a clinker containing high amount of α-C2S to replace C3S. Such a clinker may have good applications in the synthesis of active belite cement.Se estudia con detalle la influencia del P2O5 sobre las composiciones mineralógicas en fases silicato y en un crudo industrial. Asimismo se estudian algunas composiciones de la zona rica en cal del sistema CaO-SiO2-P2O5 utilizando reactivos químicos. Los resultados demuestran que, a presión atmosférica y recinto abierto, las composiciones dentro de los límites CaO = 69,5-70,1; P2O5= 16,2-19,5; SiO2 = 11,1-13,6 (% en peso dan a 1000ºC la composición mineralógica siguiente: HAp, β-C2S, C5PS; pueden desarrollar una alta resistencia química en "chemically bonded ceramics". A altas temperaturas (1400°C producen una elevada proporción de silicocarnotita (C5PS S y la fase 2C2.C3P. Con la adición de fosfato a una determinada dosificación de crudo industrial (CaO = 62,66; SiO2 = 17,33; Al2O3 = 4,88; Fe2O3 = 4,29; P2O5 = 8,73; MgO = 2,09 -% en peso- se forma, a 1350°C, un clinker que contiene una alta proporción de α-C2S que reemplaza al C3S. Este clinker puede tener buenas aplicaciones en la obtención de

  12. miR-4782-3p Inhibited Non-Small Cell Lung Cancer growth via USP14

    Directory of Open Access Journals (Sweden)

    Ning Wu

    2014-02-01

    Full Text Available Background: Lung cancer is the leading cause of cancer-related mortality worldwide, with near 1.4 million deaths each year. NSCLC accounts for nearly 85% of all case of lung cancer. MiRNAs play important roles in regulation of gene expression at the post-transcriptional level. MiRNAs profiles may predict prognosis and disease recurrence in early-stage NSCLC. Our previous study proved that over-expression of ubiquitin specific peptidase 14 (USP14, a deubiquitinating enzyme, was associated with favorable prognosis in NSCLC patients and promoted tumor cells proliferation. Here, we tried to identify which miRNAs targeted USP14, and the roles of these miRNAs in NSCLC. Methods: MiR-4782-3p and its potential targeted genes were identified by bioinformatics algorithm. Dual luciferase reporter assay system was used to analyze the interaction between miR-4782-3p and targeted genes. Cell proliferation was assayed by MTT and BdU assay. MiRNAs and mRNA expression were assayed by qRT-PCR. USP14 protein level was assayed by Western blot. The role of miR-4782-3p in patients survival was revealed by Kaplan-Meier plot of overall survival. Results: Up-expression of miR-4782-3p in NSCLC cells decreased the USP14 expression. Down-expression of miR-4782-3p increased USP14 expression. In NSCLC specimen, Negative correlation between USP14 mRNA level and miR-4782-3p level was identified. Higher miR-4782-3p expression is associated with longer survival. USP14, ZEB2, XIAP overexpression reversed the inhibitory effect of miR-4782-3p. Conclusions: High expression of miR-4782-3p was associated with favorable prognosis in NSCLC patients. MiR-4782-3p inhibited cell proliferation in NSCLC by targeting USP14, ZEB2 and XIAP.

  13. Susceptibility loci for polycystic ovary syndrome on chromosome 2p16.3, 2p21, and 9q33.3 in a cohort of Caucasian women.

    Science.gov (United States)

    Lerchbaum, E; Trummer, O; Giuliani, A; Gruber, H-J; Pieber, T R; Obermayer-Pietsch, B

    2011-10-01

    In a recent genome-wide association study investigating Han Chinese PCOS women 3 loci that are strongly associated with PCOS were identified on chromosome 2p16.3 (rs13405728), 2p21 (rs13429458), and 9q33.3 (rs2479106). The aim of the study was to investigate the impact of rs13405728, rs13429458, and rs2479106 variants on PCOS susceptibility in a Caucasian cohort of PCOS and control women. Metabolic, endocrine, and anthropometric measurements and oral glucose tolerance tests were performed in 545 PCOS and 317 control women. The rs13405728, rs13429458, and rs2479106 polymorphisms were genotyped. There was no significant difference in genotype frequencies of rs13405728 and rs13429458 variants between PCOS and controls. There was a trend towards an association of the rs2479106 variant with PCOS susceptibility (p=0.053). PCOS women with the rs2479106 GG genotype had significantly higher WHR than PCOS women carrying the AG and AA genotype (p=0.034 and p=0.020, respectively). Moreover, QChol/HDL and LDL levels were significantly higher in PCOS women carrying the rs2479106 GG genotype when compared to those carrying the AA genotype (p=0.024 and p=0.035, respectively). PCOS women carrying the G allele of rs13405728 had significantly higher AUCgluc, glucose-30 min, and AUCins levels than those carrying the AA genotype (p=0.039, p=0.047, and p=0.044, respectively). In PCOS women, rs13405728 genotypes are associated with glucose and insulin metabolism. Moreover, rs2479106 genotypes were associated with increased WHR levels and an adverse serum lipid profile. Further, we observed a trend towards decreased PCOS susceptibility within carriers of the rs2479106 G-allele. Further studies in large Caucasian PCOS cohorts are warranted to confirm our findings. © Georg Thieme Verlag KG Stuttgart · New York.

  14. Electron-impact excitation of the (2p2) 1D and (2s2p) 1Po autoionizing states of helium

    Science.gov (United States)

    Sise, Omer; Dogan, Mevlut; Okur, Ibrahim; Crowe, Albert

    2011-08-01

    An experimental study of the excitation of the (2p2) 1D and (2s2p) 1Po autoionizing states of helium by 250-eV electron impact is presented. The ejected-electron angular distributions and energy spectra are measured in coincidence with the corresponding scattered electrons for a scattering angle of -13∘ and for a range of ejected-electron angles in both the forward and backward directions. Resonance profiles are analyzed in terms of the Shore-Balashov parametrization to obtain the resonance asymmetry Aμ and yield Bμ parameters and the direct ionization cross section f. The spectra and their parameters are compared to the previous measurements of Lower and Weigold [J. Phys. B.JPAPEH0953-407510.1088/0953-4075/23/16/023 23, 2819 (1990)] and McDonald and Crowe [J. Phys. BJPAPEH0953-407510.1088/0953-4075/26/17/021 26, 2887 (1993)]. Comparison is also made with the recent theoretical triply differential cross-section calculations based on the first and second Born approximations. In general, good qualitative agreement is found between the experimental results. Some differences are found at the forward and backward directions. These differences in the shape and magnitude of the cross sections are attributed to the different incoming electron energies used in the experiments. The second Born approximation with inclusion of the three-body Coulomb interaction in the final state agrees reasonably well with experiments in the binary region. However, the 1Po resonance yield parameter Bμ is significantly overestimated at the recoil region, giving a relatively large recoil peak, in contradiction to the experiment. There is also a discrepancy between the two theories available for the 1D resonance yield parameter Bμ in this region. Remaining discrepancies between theories and experiments are also discussed.

  15. Comparing manually-developed and data-driven rules for P2P learning

    CSIR Research Space (South Africa)

    Loots, L

    2009-11-01

    Full Text Available Phoneme-to-phoneme (P2P) learning provides a mechanism for predicting the pronunciation of a word based on its pronunciation in a different accent, dialect or language. The authors evaluate the effectiveness of manually-developed as well...

  16. Database selection and result merging in P2P web search

    NARCIS (Netherlands)

    Chernov, S.; Serdyukov, P.; Bender, M.; Michel, S.; Weikum, G.; Zimmer, C.

    2005-01-01

    Intelligent Web search engines are extremely popular now. Currently, only the commercial centralized search engines like Google can process terabytes of Web data. Alternative search engines fulfilling collaborative Web search on a voluntary basis are usually based on a blooming Peer-to-Peer (P2P) te

  17. Is the X (3915 ) the χc 0(2 P )?

    Science.gov (United States)

    Olsen, Stephen Lars

    2015-03-01

    The Particle Data Group Meson Summary Table lists the X (3915 ) meson, an ω J /ψ mass peak seen in B →K ω J /ψ decays and γ γ →ω J /ψ two-photon fusion reactions, as the χc 0(2 P ), the 23P0 charmonium state. Here, with some reasonable assumptions, it is shown that if the X (3915 ) is the χc 0(2 P ), the measured strength of the γ γ →X (3915 ) signal implies an upper limit on the branching fraction B (χc 0(2 P )→ω J /ψ ) that is below a lower limit inferred for the same quantity from the B →K X (3915 ) decay rate. Also, the absence any signal for X (3915 )→D0D¯0 in B+→K+D0D¯0 decays is used to infer the limit B (X (3915 )→D0D¯0)Zweig-Iizuka suppressed, should be relatively rare. These, plus reasons given earlier by Guo and Meissner, raise concerns about the X (3915 )=χc 0(2 P ) assignment.

  18. Scaling laws for file dissemination in P2P networks with random contacts

    NARCIS (Netherlands)

    Núñez-Queija, R.; Prabhu, B.

    2008-01-01

    In this paper we obtain the scaling law for the mean broadcast time of a file in a P2P network with an initial population of N nodes. In the model, at Poisson rate lambda a node initiates a contact with another node chosen uniformly at random. This contact is said to be successful if the contacted n

  19. Thermodynamics around the first-order ferromagnetic phase transition of Fe2P single crystals

    NARCIS (Netherlands)

    Hudl, M.; Campanini, D.; Caron, L.; Höglin, V.; Sahlberg, M.; Nordblad, P.; Rydh, A.

    2014-01-01

    The specific heat and thermodynamics of Fe2P single crystals around the first-order paramagnetic to ferromagnetic (FM) phase transition at TC≃217K are empirically investigated. The magnitude and direction of the magnetic field relative to the crystal axes govern the derived H−T phase diagram. Striki

  20. Load Balancing Scheme on the Basis of Huffman Coding for P2P Information Retrieval

    Science.gov (United States)

    Kurasawa, Hisashi; Takasu, Atsuhiro; Adachi, Jun

    Although a distributed index on a distributed hash table (DHT) enables efficient document query processing in Peer-to-Peer information retrieval (P2P IR), the index costs a lot to construct and it tends to be an unfair management because of the unbalanced term frequency distribution. We devised a new distributed index, named Huffman-DHT, for P2P IR. The new index uses an algorithm similar to Huffman coding with a modification to the DHT structure based on the term distribution. In a Huffman-DHT, a frequent term is assigned to a short ID and allocated a large space in the node ID space in DHT. Throuth ID management, the Huffman-DHT balances the index registration accesses among peers and reduces load concentrations. Huffman-DHT is the first approach to adapt concepts of coding theory and term frequency distribution to load balancing. We evaluated this approach in experiments using a document collection and assessed its load balancing capabilities in P2P IR. The experimental results indicated that it is most effective when the P2P system consists of about 30, 000 nodes and contains many documents. Moreover, we proved that we can construct a Huffman-DHT easily by estimating the probability distribution of the term occurrence from a small number of sample documents.