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Sample records for 250-ghz gyrotron dnp

  1. 500-fold enhancement of in situ 13C liquid state NMR using gyrotron-driven temperature-jump DNP

    Science.gov (United States)

    Yoon, Dongyoung; Soundararajan, Murari; Caspers, Christian; Braunmueller, Falk; Genoud, Jérémy; Alberti, Stefano; Ansermet, Jean-Philippe

    2016-09-01

    A 550-fold increase in the liquid state 13C NMR signal of a 50 μL sample was obtained by first hyperpolarizing the sample at 20 K using a gyrotron (260 GHz), then, switching its frequency in order to apply 100 W for 1.5 s so as to melt the sample, finally, turning off the gyrotron to acquire the 13C NMR signal. The sample stays in its NMR resonator, so the sequence can be repeated with rapid cooling as the entire cryostat stays cold. DNP and thawing of the sample are performed only by the switchable and tunable gyrotron without external devices. Rapid transition from DNP to thawing in one second time scale was necessary especially in order to enhance liquid 1H NMR signal.

  2. Further Characterization of 394-GHz Gyrotron FU CW GII with Additional PID Control System for 600-MHz DNP-SSNMR Spectroscopy

    Science.gov (United States)

    Ueda, Keisuke; Matsuki, Yoh; Fujiwara, Toshimichi; Tatematsu, Yoshinori; Ogawa, Isamu; Idehara, Toshitaka

    2016-09-01

    A 394-GHz gyrotron, FU CW GII, has been designed at the University of Fukui, Japan, for dynamic nuclear polarization (DNP)-enhanced solid-state nuclear magnetic resonance (SSNMR) experiments at 600-MHz 1H resonant frequency. After installation at the Institute for Protein Research (IPR), Osaka University, Japan, a PID feedback control system was equipped to regulate the electron gun heater current for stabilization of the electron beam current, which ultimately achieved stabilization of output power when operating in continuous wave (CW) mode. During exploration to further optimize operating conditions, a continuous tuning bandwidth of approximately 1 GHz was observed by varying the operating voltage at a fixed magnetic field. In the frequency range required for positive DNP enhancement, the output power was improved by increasing the magnetic field and the operating voltage from their initial operational settings. In addition, fine tuning of output frequency by varying the cavity cooling water temperature was demonstrated. These operating conditions and ancillary enhancements are expected to contribute to further enhancement of SSNMR signal.

  3. Gyrotron development at KIT: FULGOR test facility and gyrotron concepts for DEMO

    Energy Technology Data Exchange (ETDEWEB)

    Schmid, M., E-mail: martin.schmid@kit.edu [Institute for Pulsed Power and Microwave Technology (IHM), Karlsruhe Institute of Technology, Association EURATOM-KIT, Karlsruhe (Germany); Franck, J.; Kalaria, P.; Avramidis, K.A.; Gantenbein, G.; Illy, S. [Institute for Pulsed Power and Microwave Technology (IHM), Karlsruhe Institute of Technology, Association EURATOM-KIT, Karlsruhe (Germany); Jelonnek, J. [Institute for Pulsed Power and Microwave Technology (IHM), Karlsruhe Institute of Technology, Association EURATOM-KIT, Karlsruhe (Germany); Institute of High Frequency Techniques and Electronics (IHE), Karlsruhe Institute of Technology, Association EURATOM-KIT, Karlsruhe (Germany); Pagonakis, I. Gr.; Rzesnicki, T. [Institute for Pulsed Power and Microwave Technology (IHM), Karlsruhe Institute of Technology, Association EURATOM-KIT, Karlsruhe (Germany); Thumm, M. [Institute for Pulsed Power and Microwave Technology (IHM), Karlsruhe Institute of Technology, Association EURATOM-KIT, Karlsruhe (Germany); Institute of High Frequency Techniques and Electronics (IHE), Karlsruhe Institute of Technology, Association EURATOM-KIT, Karlsruhe (Germany)

    2015-10-15

    Highlights: • Substantial extension of the KIT gyrotron test facility FULGOR has started. • FULGOR will be able to test gyrotrons with continuous RF output power up to 4 MW. • Design of 240 GHz gyrotrons for efficient electron cyclotron current drive is progressing. • Output power of 240 GHz gyrotrons with conventional cavity up to 830 kW, with coaxial cavity up to 2 MW is feasible. • Multi-frequency operation with gyrotrons is also possible (170–267 GHz). - Abstract: At the Karlsruhe Institute of Technology (KIT), theoretical and experimental foundations for the development of future gyrotrons for fusion applications are being laid down. This includes the construction of the new Fusion Long Pulse Gyrotron Laboratory (FULGOR) test facility as well as physical design studies towards DEMO-compatible gyrotrons. Initially FULGOR will comprise of a 10 MW CW power supply, a 5 MW water cooling system (upgradeable to 10 MW), a superconducting 10 T magnet, one or two 2 MW ECRH test loads and a new control and data acquisition system for all these elements. The test facility will then be equipped to test the conventional 1 MW or coaxial 2 MW gyrotrons for DEMO, currently under design, as well as possible upgraded gyrotrons for W7-X and ITER. The design of the new high voltage DC power supply (HVDCPS) is flexible enough to handle gyrotrons with 4 MW CW output power (conceivably up to 170 GHz), but also test gyrotrons with higher frequencies (>250 GHz) which, due to physical limitations in the gyrotron design, will require less power but have more stringent demands on voltage stability.

  4. Design of an electronically tunable millimeter wave Gyrotron Backward Wave Oscillator

    Energy Technology Data Exchange (ETDEWEB)

    Caplan, M.

    1987-01-01

    A non-linear self-consistent computer simulation code is used to analyze the saturated output of the Gyrotron Backward Wave Oscillator (Gyro BWO) which can be used as a tunable driver for a 250 GHz FEL amplifier. Simulations show that the Gyrotron BWO using a Pierce/Wiggler gun configuration can produce at least 10 kW of microwave power over the range 249 GHz to 265 GHz by varying beam voltage alone.

  5. Design of an electronically tunable millimeter wave Gyrotron Backward Wave Oscillator

    Energy Technology Data Exchange (ETDEWEB)

    Caplan, M.

    1987-01-01

    A non-linear self-consistent computer simulation code is used to analyze the saturated output of the Gyrotron Backward Wave Oscillator (Gyro BWO) which can be used as a tunable driver for a 250 GHz FEL amplifier. Simulations show that the Gyrotron BWO using a Pierce/Wiggler gun configuration can produce at least 10 kW of microwave power over the range 249 GHz to 265 GHz by varying beam voltage alone.

  6. Simulations of the Performance of the Fem Oscillator for Fusion at 130-250 Ghz

    NARCIS (Netherlands)

    Tulupov, A. V.; van der Wiel, M. J.; Urbanus, W. H.; Caplan, M.

    1994-01-01

    The performance of a 1 MW, long pulse, 130-250 GHz free-electron maser being designed in the FOM-Institute of Plasmaphysics for fusion applications has been simulated by a fully 3-D, AC and DC space charge included, non-wiggle averaged, particle-pusher code. In comparison with previous designs a new

  7. First demonstration of a vehicle mounted 250GHz real time passive imager

    Science.gov (United States)

    Mann, Chris

    2009-05-01

    This paper describes the design and performance of a ruggedized passive Terahertz imager, the frequency of operation is a 40GHz band centred around 250GHz. This system has been specifically targeted at vehicle mounted operation, outdoors in extreme environments. The unit incorporates temperature stabilization along with an anti-vibration chassis and is sealed to allow it to be used in a dusty environment. Within the system, a 250GHz heterodyne detector array is mated with optics and scanner to allow real time imaging out to 100 meters. First applications are envisaged to be stand-off, person borne IED detection to 30 meters but the unique properties in this frequency band present other potential uses such as seeing through smoke and fog. The possibility for use as a landing aid is discussed. A detailed description of the system design and video examples of typical imaging output will be presented.

  8. Predicted Performance of a Dc Beam Driven Fem Oscillator Designed for Fusion Applications at 200-250 Ghz

    NARCIS (Netherlands)

    Caplan, M.; Best, R. W. B.; Verhoeven, A. G. A.; van der Wiel, M. J.; Urbanus, W. H.; Bratman, V. L.; Denisov, G. G.

    1993-01-01

    At the FOM Institute (The Netherlands) a free electron laser using a dc beam with depressed collector is being developed for plasma heating, which will be capable of producing 1 MW cw output over an adjustable tuning range of 200-250 GHz. The basic design parameters for the electron beam, undulator

  9. Photonic-Band-Gap Traveling-Wave Gyrotron Amplifier

    Science.gov (United States)

    Nanni, E. A.; Lewis, S. M.; Shapiro, M. A.; Griffin, R. G.; Temkin, R. J.

    2014-01-01

    We report the experimental demonstration of a gyrotron traveling-wave-tube amplifier at 250 GHz that uses a photonic band gap (PBG) interaction circuit. The gyrotron amplifier achieved a peak small signal gain of 38 dB and 45 W output power at 247.7 GHz with an instantaneous −3 dB bandwidth of 0.4 GHz. The amplifier can be tuned for operation from 245–256 GHz. The widest instantaneous −3 dB bandwidth of 4.5 GHz centered at 253.25 GHz was observed with a gain of 24 dB. The PBG circuit provides stability from oscillations by supporting the propagation of transverse electric (TE) modes in a narrow range of frequencies, allowing for the confinement of the operating TE03-like mode while rejecting the excitation of oscillations at nearby frequencies. This experiment achieved the highest frequency of operation for a gyrotron amplifier; at present, there are no other amplifiers in this frequency range that are capable of producing either high gain or high output power. This result represents the highest gain observed above 94 GHz and the highest output power achieved above 140 GHz by any conventional-voltage vacuum electron device based amplifier. PMID:24476286

  10. Low-voltage gyrotrons

    Science.gov (United States)

    Glyavin, M. Yu.; Zavolskiy, N. A.; Sedov, A. S.; Nusinovich, G. S.

    2013-03-01

    For a long time, the gyrotrons were primarily developed for electron cyclotron heating and current drive of plasmas in controlled fusion reactors where a multi-megawatt, quasi-continuous millimeter-wave power is required. In addition to this important application, there are other applications (and their number increases with time) which do not require a very high power level, but such issues as the ability to operate at low voltages and have compact devices are very important. For example, gyrotrons are of interest for a dynamic nuclear polarization, which improves the sensitivity of the nuclear magnetic resonance spectroscopy. In this paper, some issues important for operation of gyrotrons driven by low-voltage electron beams are analyzed. An emphasis is made on the efficiency of low-voltage gyrotron operation at the fundamental and higher cyclotron harmonics. These efficiencies calculated with the account for ohmic losses were, first, determined in the framework of the generalized gyrotron theory based on the cold-cavity approximation. Then, more accurate, self-consistent calculations for the fundamental and second harmonic low-voltage sub-THz gyrotron designs were carried out. Results of these calculations are presented and discussed. It is shown that operation of the fundamental and second harmonic gyrotrons with noticeable efficiencies is possible even at voltages as low as 5-10 kV. Even the third harmonic gyrotrons can operate at voltages about 15 kV, albeit with rather low efficiency (1%-2% in the submillimeter wavelength region).

  11. DNP Communication Function with RTDS

    DEFF Research Database (Denmark)

    Cha, Seung-Tae; Wu, Qiuwei; Saleem, Arshad

    2010-01-01

    A simulation case is implemented on RSCAD for testing their communication. The case has two binary status points (mapped to DNP binary input objects 1 & 2) and two binary control points (mapped to DNP binary output objects 10 and controlled via DNP objects 12). There is also one analog status poi...

  12. Gyrotrons for High-Power Terahertz Science and Technology at FIR UF

    CERN Document Server

    Idehara, Toshitaka

    2016-01-01

    In this paper, we present the recent progress in the development of a series of gyrotrons at FIR UF that have opened the road to many novel applications in the high-power Terahertz science and technology. The current status of the research in this actively developing field is illustrated by the most representative examples in which the developed gyrotrons are used as powerful and frequency tunable sources of coherent radiation operating in a CW regime. Among them are high-precision spectroscopic techniques (most notably DNP-NMR, ESR, XDMR, and studies of the hyperfine splitting of the energy levels of positronium), treatment and characterization of advanced materials, new medical technologies.

  13. Development of simulation tools for numerical investigation and computer-aided design (CAD) of gyrotrons

    Science.gov (United States)

    Damyanova, M.; Sabchevski, S.; Zhelyazkov, I.; Vasileva, E.; Balabanova, E.; Dankov, P.; Malinov, P.

    2016-10-01

    As the most powerful CW sources of coherent radiation in the sub-terahertz to terahertz frequency range the gyrotrons have demonstrated a remarkable potential for numerous novel and prospective applications in the fundamental physical research and the technologies. Among them are powerful gyrotrons for electron cyclotron resonance heating (ECRH) and current drive (ECCD) of magnetically confined plasma in various reactors for controlled thermonuclear fusion (e.g., tokamaks and most notably ITER), high-frequency gyrotrons for sub-terahertz spectroscopy (for example NMR-DNP, XDMR, study of the hyperfine structure of positronium, etc.), gyrotrons for thermal processing and so on. Modelling and simulation are indispensable tools for numerical studies, computer-aided design (CAD) and optimization of such sophisticated vacuum tubes (fast-wave devices) operating on a physical principle known as electron cyclotron resonance maser (ECRM) instability. During the recent years, our research team has been involved in the development of physical models and problem-oriented software packages for numerical analysis and CAD of different gyrotrons in the framework of a broad international collaboration. In this paper we present the current status of our simulation tools (GYROSIM and GYREOSS packages) and illustrate their functionality by results of numerical experiments carried out recently. Finally, we provide an outlook on the envisaged further development of the computer codes and the computational modules belonging to these packages and specialized to different subsystems of the gyrotrons.

  14. Experimental tests of a 263 GHz gyrotron for spectroscopic applications and diagnostics of various media

    Energy Technology Data Exchange (ETDEWEB)

    Glyavin, M. Yu., E-mail: glyavin@appl.sci-nnov.ru; Denisov, G. G.; Zapevalov, V. E. [Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod (Russian Federation); Gycom Ltd., Nizhny Novgorod (Russian Federation); Chirkov, A. V.; Fokin, A. P.; Kholoptsev, V. V.; Kuftin, A. N.; Luchinin, A. G.; Golubyatnikov, G. Yu.; Malygin, V. I.; Morozkin, M. V.; Manuilov, V. N.; Proyavin, M. D.; Sedov, A. S.; Tsvetkov, A. I. [Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod (Russian Federation); Sokolov, E. V.; Tai, E. M. [Gycom Ltd., Nizhny Novgorod (Russian Federation)

    2015-05-15

    A 263 GHz continuous-wave (CW) gyrotron was developed at the IAP RAS for future applications as a microwave power source in Dynamic Nuclear Polarization / Nuclear magnetic resonance (DNP/NMR) spectrometers. A new experimental facility with a computerized control was built to test this and subsequent gyrotrons. We obtained the maximum CW power up to 1 kW in the 15 kV/0.4 A operation regime. The power about 10 W, which is sufficient for many spectroscopic applications, was realized in the low current 14 kV/0.02 A regime. The possibility of frequency tuning by variation of the coolant temperature about 4 MHz/1 °C was demonstrated. The spectral width of the gyrotron radiation was about 10{sup −6}.

  15. Soviet Development of Gyrotrons

    Science.gov (United States)

    1986-05-01

    Relationship Type of Device Remarks V, - Vc, anomalous Doppler Capable of 100 percent efficiency, CRM but more cumbersome than Cheren- kov devices V...authors; and discusses inlividual Soviet reseaLc- groups, the basic organizational units responAiLle for the CRM and gyrotron research and development. The...maintained a cCnEistEnt iecord of significant achievements; it has managed to overcome the systenic yeaxness of the Soviet R&C systeg in teimg atle to

  16. Gyrotron oscillators for fusion heating

    Energy Technology Data Exchange (ETDEWEB)

    Jory, H.; Evans, S.; Felch, K.; Shively, J.; Spang, S.

    1982-01-01

    Recent experiments have been performed to determine the ultimate power capability of a 28 GHz 200 kW CW gyrotron design. A power output of 342 kW CW was measured in these tests with an efficiency of 37%. Progress in the development of 60 GHz 200 kW pulsed and CW gyrotrons is discussed. An output of 200 kW with 100 msec pulse length has been achieved with the pulsed design.

  17. A spectrometer designed for 6.7 and 14.1 T DNP-enhanced solid-state MAS NMR using quasi-optical microwave transmission.

    Science.gov (United States)

    Pike, Kevin J; Kemp, Thomas F; Takahashi, Hiroki; Day, Robert; Howes, Andrew P; Kryukov, Eugeny V; MacDonald, James F; Collis, Alana E C; Bolton, David R; Wylde, Richard J; Orwick, Marcella; Kosuga, Kosuke; Clark, Andrew J; Idehara, Toshitaka; Watts, Anthony; Smith, Graham M; Newton, Mark E; Dupree, Ray; Smith, Mark E

    2012-02-01

    A Dynamic Nuclear Polarisation (DNP) enhanced solid-state Magic Angle Spinning (MAS) NMR spectrometer operating at 6.7 T is described and demonstrated. The 187 GHz TE(13) fundamental mode of the FU CW VII gyrotron is used as the microwave source for this magnetic field strength and 284 MHz (1)H DNP-NMR. The spectrometer is designed for use with microwave frequencies up to 395 GHz (the TE(16) second-harmonic mode of the gyrotron) for DNP at 14.1T (600 MHz (1)H NMR). The pulsed microwave output from the gyrotron is converted to a quasi-optical Gaussian beam using a Vlasov antenna and transmitted to the NMR probe via an optical bench, with beam splitters for monitoring and adjusting the microwave power, a ferrite rotator to isolate the gyrotron from the reflected power and a Martin-Puplett interferometer for adjusting the polarisation. The Gaussian beam is reflected by curved mirrors inside the DNP-MAS-NMR probe to be incident at the sample along the MAS rotation axis. The beam is focussed to a ~1 mm waist at the top of the rotor and then gradually diverges to give much more efficient coupling throughout the sample than designs using direct waveguide irradiation. The probe can be used in triple channel HXY mode for 600 MHz (1)H and double channel HX mode for 284 MHz (1)H, with MAS sample temperatures ≥85 K. Initial data at 6.7 T and ~1 W pulsed microwave power are presented with (13)C enhancements of 60 for a frozen urea solution ((1)H-(13)C CP), 16 for bacteriorhodopsin in purple membrane ((1)H-(13)C CP) and 22 for (15)N in a frozen glycine solution ((1)H-(15)N CP) being obtained. In comparison with designs which irradiate perpendicular to the rotation axis the approach used here provides a highly efficient use of the incident microwave beam and an NMR-optimised coil design.

  18. Recent result of gyrotron operation in NIFS

    Directory of Open Access Journals (Sweden)

    Ito Satoshi

    2015-01-01

    Full Text Available In the last Large Helical Device (LHD experimental campaign, a 154GHz gyrotron which had been conditioned to generate 1 MW/2 s, 0.5 MW/CW was installed for LHD experiments. Four high power gyrotrons (three-77 GHz/1~1.5 MW and one-154 GHz/1 MW and a CW gyrotron (84 GHz/0.2 MW are ready. Our experiment requires high energy and various injection patterns for Electron Cyclotron Resonance Heating (ECRH. Higher individual injection power and various injection patterns, we developed a power enhancement method by stepped anode acceleration voltage control and operated the gyrotron in the hard excitation region. These operations were realized by a remote controlled waveform generator. However the oscillation map of high power or long pulse operation in the hard excitation region were limited because in order to achieve the hard excitation region by the anode voltage control one must pass through the high anode current phase within a time short enough that the anode or the anode power supply is not overloaded. This limitation becomes more critical when the gyrotron beam current is increased in order to increase the output power. In the long pulse operation it was impossible to reach the hard excitation region in a low beam current (<10A.

  19. Recent result of gyrotron operation in NIFS

    Science.gov (United States)

    Ito, Satoshi; Shimozuma, Takashi; Yoshimura, Yasuo; Igami, Hiroe; Takahashi, Hiromi; Nishiura, Masaki; Kobayashi, Sakuji; Mizuno, Yoshinori; Okada, Kota; Kubo, Shin

    2015-03-01

    In the last Large Helical Device (LHD) experimental campaign, a 154GHz gyrotron which had been conditioned to generate 1 MW/2 s, 0.5 MW/CW was installed for LHD experiments. Four high power gyrotrons (three-77 GHz/1~1.5 MW and one-154 GHz/1 MW) and a CW gyrotron (84 GHz/0.2 MW) are ready. Our experiment requires high energy and various injection patterns for Electron Cyclotron Resonance Heating (ECRH). Higher individual injection power and various injection patterns, we developed a power enhancement method by stepped anode acceleration voltage control and operated the gyrotron in the hard excitation region. These operations were realized by a remote controlled waveform generator. However the oscillation map of high power or long pulse operation in the hard excitation region were limited because in order to achieve the hard excitation region by the anode voltage control one must pass through the high anode current phase within a time short enough that the anode or the anode power supply is not overloaded. This limitation becomes more critical when the gyrotron beam current is increased in order to increase the output power. In the long pulse operation it was impossible to reach the hard excitation region in a low beam current (<10A).

  20. Megawatt, 330 Hz PRF tunable gyrotron experiments

    Science.gov (United States)

    Spark, S. N.; Cross, A. W.; Phelps, A. D. R.; Ronald, K.

    1994-12-01

    Repetitively pulsed and cw gyrotrons have hitherto used thermionic cathodes, whereas cold cathode gyrotrons have normally operated as ‘single shot’ devices. The novel results presented here show that cold cathode gyrotrons can be successfully pulsed repetitively. A tunable gyrotron with a pulse repetition frequency (PRF) of 150Hz is demonstrated. This system developed >4MW mm-wave output pulses at 100GHz. The gyrotron is based on a two-electrode configuration comprising a field-immersed, field emission, cold cathode and a shaped anode cavity. A superconducting magnet was used to produce the homogeneous intra-cavity magnetic field and a cable pulser was used to drive the electron beam. This pulser produced up to a (200±20)kV pulse with 10ns rise time, a 100ns flat top, a 10ns decay with a characteristic impedance of 200Ω. The energy storage capacity of the cable pulser was 35J. The charging unit limited the maximum PRF to 330Hz. Due to spark gap switching limitations 330Hz was only obtainable in 5 to 10 pulse bursts. For substantial periods of the order of 30 seconds, 100Hz PRF was achieved over an oscillating range of 28 to 100GHz and 150Hz PRF was achieved at 80GHz. No degradation effects on the mm-wave output pulse was evident due to diode recovery time throughout this series of results. A subsequent conclusion is that the diode recovery time in our cold cathode gyrotron is less than 3ms.

  1. A study of a simple gyrotron equation

    Energy Technology Data Exchange (ETDEWEB)

    Goetz, Markus [Max-Planck-Institut fuer Plasmaphysik, EURATOM-Association, D-85748 Garching (Germany); Meyer-Spasche, Rita [Max-Planck-Institut fuer Plasmaphysik, EURATOM-Association, D-85748 Garching (Germany); Weitzner, Harold [New York University, Courant Institute of Mathematical Sciences, New York, NY 10012 (United States)

    2007-03-02

    A simple standard equation for the evolution of the electrons and electromagnetic fields in a gyrotron cavity is studied. A number of mathematical properties are shown: existence and uniqueness of solutions for a limited axial extent and existence and uniqueness for all axial lengths in one case of particular interest. A Poynting theorem is obtained directly from the model and the Hamiltonian character of the electron motion is demonstrated. The start-up and final state in the gyrotron cavity are also examined. The efficiency of energy flux transfer from the electron beam to the wave is estimated.

  2. Ultra-low temperature MAS-DNP

    Science.gov (United States)

    Lee, Daniel; Bouleau, Eric; Saint-Bonnet, Pierre; Hediger, Sabine; De Paëpe, Gaël

    2016-03-01

    Since the infancy of NMR spectroscopy, sensitivity and resolution have been the limiting factors of the technique. Regular essential developments on this front have led to the widely applicable, versatile, and powerful spectroscopy that we know today. However, the Holy Grail of ultimate sensitivity and resolution is not yet reached, and technical improvements are still ongoing. Hence, high-field dynamic nuclear polarization (DNP) making use of high-frequency, high-power microwave irradiation of electron spins has become very promising in combination with magic angle sample spinning (MAS) solid-state NMR experiments. This is because it leads to a transfer of the much larger polarization of these electron spins under suitable irradiation to surrounding nuclei, greatly increasing NMR sensitivity. Currently, this boom in MAS-DNP is mainly performed at minimum sample temperatures of about 100 K, using cold nitrogen gas to pneumatically spin and cool the sample. This Perspective deals with the desire to improve further the sensitivity and resolution by providing "ultra"-low temperatures for MAS-DNP, using cryogenic helium gas. Different designs on how this technological challenge has been overcome are described. It is shown that stable and fast spinning can be attained for sample temperatures down to 30 K using a large cryostat developed in our laboratory. Using this cryostat to cool a closed-loop of helium gas brings the additional advantage of sample spinning frequencies that can greatly surpass those achievable with nitrogen gas, due to the differing fluidic properties of these two gases. It is shown that using ultra-low temperatures for MAS-DNP results in substantial experimental sensitivity enhancements and according time-savings. Access to this temperature range is demonstrated to be both viable and highly pertinent.

  3. Towards Overhauser DNP in supercritical CO2

    Science.gov (United States)

    van Meerten, S. G. J.; Tayler, M. C. D.; Kentgens, A. P. M.; van Bentum, P. J. M.

    2016-06-01

    Overhauser Dynamic Nuclear Polarization (ODNP) is a well known technique to improve NMR sensitivity in the liquid state, where the large polarization of an electron spin is transferred to a nucleus of interest by cross-relaxation. The efficiency of the Overhauser mechanism for dipolar interactions depends critically on fast local translational dynamics at the timescale of the inverse electron Larmor frequency. The maximum polarization enhancement that can be achieved for 1H at high magnetic fields benefits from a low viscosity solvent. In this paper we investigate the option to use supercritical CO2 as a solvent for Overhauser DNP. We have investigated the diffusion constants and longitudinal nuclear relaxation rates of toluene in high pressure CO2. The change in 1H T1 by addition of TEMPO radical was analyzed to determine the Overhauser cross-relaxation in such a mixture, and is compared with calculations based on the Force Free Hard Sphere (FFHS) model. By analyzing the relaxation data within this model we find translational correlation times in the range of 2-4 ps, depending on temperature, pressure and toluene concentration. Such short correlation times may be instrumental for future Overhauser DNP applications at high magnetic fields, as are commonly used in NMR. Preliminary DNP experiments have been performed at 3.4 T on high pressure superheated water and model systems such as toluene in high pressure CO2.

  4. Towards Overhauser DNP in supercritical CO2.

    Science.gov (United States)

    van Meerten, S G J; Tayler, M C D; Kentgens, A P M; van Bentum, P J M

    2016-06-01

    Overhauser Dynamic Nuclear Polarization (ODNP) is a well known technique to improve NMR sensitivity in the liquid state, where the large polarization of an electron spin is transferred to a nucleus of interest by cross-relaxation. The efficiency of the Overhauser mechanism for dipolar interactions depends critically on fast local translational dynamics at the timescale of the inverse electron Larmor frequency. The maximum polarization enhancement that can be achieved for (1)H at high magnetic fields benefits from a low viscosity solvent. In this paper we investigate the option to use supercritical CO2 as a solvent for Overhauser DNP. We have investigated the diffusion constants and longitudinal nuclear relaxation rates of toluene in high pressure CO2. The change in (1)H T1 by addition of TEMPO radical was analyzed to determine the Overhauser cross-relaxation in such a mixture, and is compared with calculations based on the Force Free Hard Sphere (FFHS) model. By analyzing the relaxation data within this model we find translational correlation times in the range of 2-4ps, depending on temperature, pressure and toluene concentration. Such short correlation times may be instrumental for future Overhauser DNP applications at high magnetic fields, as are commonly used in NMR. Preliminary DNP experiments have been performed at 3.4T on high pressure superheated water and model systems such as toluene in high pressure CO2.

  5. Structural biology applications of solid state MAS DNP NMR

    Science.gov (United States)

    Akbey, Ümit; Oschkinat, Hartmut

    2016-08-01

    Dynamic Nuclear Polarization (DNP) has long been an aim for increasing sensitivity of nuclear magnetic resonance (NMR) spectroscopy, delivering spectra in shorter experiment times or of smaller sample amounts. In recent years, it has been applied in magic angle spinning (MAS) solid-state NMR to a large range of samples, including biological macromolecules and functional materials. New research directions in structural biology can be envisaged by DNP, facilitating investigations on very large complexes or very heterogeneous samples. Here we present a summary of state of the art DNP MAS NMR spectroscopy and its applications to structural biology, discussing the technical challenges and factors affecting DNP performance.

  6. High harmonic terahertz confocal gyrotron with nonuniform electron beam

    Energy Technology Data Exchange (ETDEWEB)

    Fu, Wenjie; Guan, Xiaotong; Yan, Yang [THz Research Center, School of Physical Electronics, University of Electronic Science and Technology of China, Chengdu 610054 (China)

    2016-01-15

    The harmonic confocal gyrotron with nonuniform electron beam is proposed in this paper in order to develop compact and high power terahertz radiation source. A 0.56 THz third harmonic confocal gyrotron with a dual arc section nonuniform electron beam has been designed and investigated. The studies show that confocal cavity has extremely low mode density, and has great advantage to operate at high harmonic. Nonuniform electron beam is an approach to improve output power and interaction efficiency of confocal gyrotron. A dual arc beam magnetron injection gun for designed confocal gyrotron has been developed and presented in this paper.

  7. A review on the sub-THz/THz gyrotrons

    Science.gov (United States)

    Kumar, Nitin; Singh, Udaybir; Bera, Anirban; Sinha, A. K.

    2016-05-01

    A review on the sub-THz/THz gyrotrons is performed in this manuscript. The present development status of gyrotrons can be divided into three streams for the sake of better understanding: 1. low frequency (industrial applications, 2. very high power (1 MW or more), medium frequency (100-200 GHz) gyrotrons for plasma fusion applications, 3. low power (few tens of watt to kW), high frequency (>200 GHz) gyrotrons for various innovative applications. In this manuscript, the third stream of gyrotron development is reviewed. In last few decades several innovative applications are searched in sub-THz/THz band where the gyrotrons could be used as an efficient source of RF radiation. The applications of sub-THz/THz gyrotrons including the futuristic scope of the device are also discussed in this article. Further, several criticalities arise in the design and development when the gyrotron operation shifts toward the high frequency band. Various such design and technological challenges are also discussed here. Finally the development status of sub-THz/THz gyrotrons as per the use in various scientific and technological applications is also discussed.

  8. Theory and Modeling of High-Power Gyrotrons

    Energy Technology Data Exchange (ETDEWEB)

    Nusinovich, Gregory Semeon [Univ. of Maryland, College Park, MD (United States)

    2016-04-29

    This report summarized results of the work performed at the Institute for Research in Electronics and Applied Physics of the University of Maryland (College Park, MD) in the framework of the DOE Grant “Theory and Modeling of High-Power Gyrotrons”. The report covers the work performed in 2011-2014. The research work was performed in three directions: - possibilities of stable gyrotron operation in very high-order modes offering the output power exceeding 1 MW level in long-pulse/continuous-wave regimes, - effect of small imperfections in gyrotron fabrication and alignment on the gyrotron efficiency and operation, - some issues in physics of beam-wave interaction in gyrotrons.

  9. Enhanced performance large volume dissolution-DNP

    DEFF Research Database (Denmark)

    Bowen, Sean; Ardenkjær-Larsen, Jan Henrik

    2014-01-01

    A systematic study of the performance of the dissolution process in dissolution-DNP is presented. A relatively simple set of modifications is made to the standard Hypersense dissolution system to enable polarization of large volume samples. These consist of a large volume sample cup along...... with supporting modifications to the dissolution head and related components. Additional modifications were made to support the mapping of the temperature/pressure space of the dissolution process as well as enabling the use of large volumes of solvent and improving the robustness of the system. No loss...

  10. Scaling Calculations for a Relativistic Gyrotron.

    Science.gov (United States)

    2014-09-26

    a relativistic gyrotron. The results of calculations are given in Section 3. The non- linear , slow-time-scale equations of motion used for these...corresponds to a cylindrical resonator and a thin annular electron beam ;, " with the beam radius chosen to coincide with a maximum of the resonator...entering the cavity. A tractable set of non- linear equations based on a slow-time-scale formulation developed previously was used. For this

  11. Continuously tunable, split-cavity gyrotrons

    Science.gov (United States)

    Brand, G. F.; Gross, M.

    1985-12-01

    Attention is given to a gyrotron cavity configuration which is split in halves longitudinally, to allow any frequency lying between the fixed cavity resonance to be assessed by mechanically changing the separation of the two halves. Experimental results are presented which demonstrate that the rate-of-change in resonant frequency with separation is greatest if the minor axis of the cavity cross section is the one undergoing change. Excellent agreement with theory is noted for these results.

  12. Innovation on high-power long-pulse gyrotrons

    Science.gov (United States)

    Litvak, Alexander; Sakamoto, Keishi; Thumm, Manfred

    2011-12-01

    Progress in the worldwide development of high-power gyrotrons for magnetic confinement fusion plasma applications is described. After technology breakthroughs in research on gyrotron components in the 1990s, significant progress has been achieved in the last decade, in particular, in the field of long-pulse and continuous wave (CW) gyrotrons for a wide range of frequencies. At present, the development of 1 MW-class CW gyrotrons has been very successful; these are applicable for self-ignition experiments on fusion plasmas and their confinement in the tokamak ITER, for long-pulse confinement experiments in the stellarator Wendelstein 7-X (W7-X) and for EC H&CD in the future tokamak JT-60SA. For this progress in the field of high-power long-pulse gyrotrons, innovations such as the realization of high-efficiency stable oscillation in very high order cavity modes, the use of single-stage depressed collectors for energy recovery, highly efficient internal quasi-optical mode converters and synthetic diamond windows have essentially contributed. The total tube efficiencies are around 50% and the purity of the fundamental Gaussian output mode is 97% and higher. In addition, activities for advanced gyrotrons, e.g. a 2 MW gyrotron using a coaxial cavity, multi-frequency 1 MW gyrotrons and power modulation technology, have made progress.

  13. Metabolic pathway visualization in living yeast by DNP-NMR.

    Science.gov (United States)

    Meier, Sebastian; Karlsson, Magnus; Jensen, Pernille R; Lerche, Mathilde H; Duus, Jens Ø

    2011-10-01

    Central carbon metabolism of living Saccharomyces cerevisiae is visualized by DNP-NMR. Experiments are conducted as real time assays that detect metabolic bottlenecks, pathway use, reversibility of reactions and reaction mechanisms in vivo with subsecond time resolution.

  14. Russian roulette with unlicensed fat-burner drug 2,4-dinitrophenol (DNP): evidence from a multidisciplinary study of the internet, bodybuilding supplements and DNP users

    National Research Council Canada - National Science Library

    Petróczi, Andrea; Ocampo, Jorge A Vela; Shah, Iltaf; Jenkinson, Carl; New, Rachael; James, Ricky A; Taylor, Glenn; Naughton, Declan P

    2015-01-01

    .... Using multiple search-engines and guidance for Internet research, online retailers and bodybuilding forums/blogs were systematically explored for availability of DNP, advice offered on DNP use and user profiles...

  15. Solvent signal suppression for high-resolution MAS-DNP

    Science.gov (United States)

    Lee, Daniel; Chaudhari, Sachin R.; De Paëpe, Gaël

    2017-05-01

    Dynamic nuclear polarization (DNP) has become a powerful tool to substantially increase the sensitivity of high-field magic angle spinning (MAS) solid-state NMR experiments. The addition of dissolved hyperpolarizing agents usually results in the presence of solvent signals that can overlap and obscure those of interest from the analyte. Here, two methods are proposed to suppress DNP solvent signals: a Forced Echo Dephasing experiment (FEDex) and TRAnsfer of Populations in DOuble Resonance Echo Dephasing (TRAPDORED) NMR. These methods reintroduce a heteronuclear dipolar interaction that is specific to the solvent, thereby forcing a dephasing of recoupled solvent spins and leaving acquired NMR spectra free of associated resonance overlap with the analyte. The potency of these methods is demonstrated on sample types common to MAS-DNP experiments, namely a frozen solution (of L-proline) and a powdered solid (progesterone), both containing deuterated glycerol as a DNP solvent. The proposed methods are efficient, simple to implement, compatible with other NMR experiments, and extendable past spectral editing for just DNP solvents. The sensitivity gains from MAS-DNP in conjunction with FEDex or TRAPDORED then permits rapid and uninterrupted sample analysis.

  16. Human plasma DNP level after severe brain injury

    Institute of Scientific and Technical Information of China (English)

    GAO Yi-lu; XIN Hui-ning; FENG Yi; FAN Ji-wei

    2006-01-01

    Objective: To determine the relationship between DNP level after human severe brain injury and hyponatremia as well as isorrhea.Methods: The peripheral venous plasma as control was collected from 8 volunteers. The peripheral venous plasma from 14 severe brain injury patients were collected in the 1, 3, 7 days after injury. Radioimmunoassay was used to detect the DNP concentration. Meanwhile, daily plasma and urine electrolytes, osmotic pressure as well as 24 h liquid intake and output volume were detected.Results: The normal adult human plasma DNP level was 62. 46 pg/ml ± 27. 56 pg/ml. In the experimental group, the plasma DNP levels were higher from day 1 today 3 in 8 of the 14 patients than those in the control group (P1 =0.05, P3 =0.03). Negative fluid balance occurred in 8 patients and hyponatremia in 7 patients. The increase of plasma DNP level was significantly correlated with the development of a negative fluid balance (r=-0.69,P<0.01) and hyponatremia (x2 =4.38, P<0.05).Conclusions: The increase of plasma DNP level is accompanied by the enhancement of natriuretic and diuretic responses in severe brain-injured patients, which is associated with the development of a negative fluid balance and hyponatremia after brain injury.

  17. A Study of the Perceptions of Doctor of Nursing (DNP) Program Administrators regarding the Integration of Acupuncture and Acupressure in DNP Curricula

    Science.gov (United States)

    Voight, Rebecca W.

    2012-01-01

    The purpose of this quantitative descriptive study was to explore the perceptions of DNP administrators regarding the extent acupuncture and acupressure are addressed in DNP curricula. Five research questions were addressed: 1. What are the perspectives of DNP program administrators regarding acupuncture and acupressure? 2. What are the…

  18. Gyrotrons for magnetic fusion applications at 110 GHz and 170 GHz

    Directory of Open Access Journals (Sweden)

    Cahalan P.

    2012-09-01

    Full Text Available Two megawatt-class gyrotrons at frequencies of 110 GHz and 170 GHz have recently been fabricated at CPI. The 110 GHz gyrotron is designed to produce 1.2 MW of output power for 10-second pulses, and will be used for electron cyclotron heating and current drive on the DIII-D tokamak at General Atomics. This gyrotron has completed factory testing and has been delivered to General Atomics for installation and additional testing. The 170 GHz gyrotron, though specified as a 500 kW CW system, has been designed with the goal of generating up to 1 MW CW. Oak Ridge National Laboratory will use this gyrotron in ITER ECH transmission line testing. This gyrotron has been fabricated and is awaiting factory testing, Design features of each gyrotron are described, and test data for the 110 GHz gyrotron are presented.

  19. Educating DNP students about critical appraisal and knowledge translation.

    Science.gov (United States)

    Moore, Elizabeth R; Watters, Richard

    2013-01-01

    Consumers expect that health care providers will use the best evidence when assisting them in making decisions about treatment options. Nurses at all educational levels report that they lack knowledge to critically appraise research studies and the skills to effectively implement evidence-based practice (EBP) in their clinical settings. Organizational culture and management support of innovation are critical factors in the adoption of EBP. Doctor of Nursing Practice (DNP) graduates can have a pivotal role in the transfer of knowledge to practice, yet critical appraisal and EBP competencies for DNP and Master of Science in Nursing (MSN) students have not been well differentiated in nursing curricula. Also students' attitudes toward EBP, self-efficacy beliefs, utilization, and knowledge gaps are rarely evaluated before courses are designed. This article reports on the development of a DNP-level EBP course to help students evaluate and apply research findings to clinical practice.

  20. On the present and future of dissolution-DNP

    DEFF Research Database (Denmark)

    Ardenkjær-Larsen, Jan Henrik

    2016-01-01

    Dissolution-DNP is a method to create solutions of molecules with nuclear spin polarization close to unity. The many orders of magnitude signal enhancement have enabled many new applications, in particular in vivo MR metabolic imaging. The method relies on solid state dynamic nuclear polarization...... at low temperature followed by a dissolution to produce the room temperature solution of highly polarized spins. This work describes the present and future of dissolution-DNP in the mind of the author. The article describes some of the current trends in the field as well as outlines some of the areas...

  1. Influence of pressure on the ferroelectric phase transition in a symmetrical polymerizable diacetylene crystal DNP

    Science.gov (United States)

    Even, J.; Bertault, M.; Girard, A.; Délugeard, Y.

    1996-12-01

    DNP is a symmetrical disubstituted polymerizable diacetylene RCCCCR where R is CH 2O(NO 2) 2. The monomer crystal of DNP undergoes a ferroelectric phase transition at low temperature; it disappears in fully polymerized DNP crystal because polymerization changes the diacetylene backbone conformation. We show that hydrostatic isotropic pressure also stabilizes the ferroelectric phase in the DNP monomer crystal by enhancing van der Waals interactions between side groups.

  2. Further studies of a simple gyrotron equation: nonlinear theory

    Energy Technology Data Exchange (ETDEWEB)

    Shi Meixuan, E-mail: meixuan@cims.nyu.ed [Courant Institute of Mathematical Sciences, New York University, 251 Mercer Street, New York, NY 10012-1185 (United States)

    2010-11-05

    A nonlinear version of a standard system of gyrotron model equations is studied using asymptotic analysis and variational methods. The condition for obtaining a high-amplitude wave is achieved in the study. A simple method for obtaining the patterns and amplitude of the wave based on the given free-space wave-number pattern is shown.

  3. The development of practice scholarship in DNP programs: a paradigm shift.

    Science.gov (United States)

    Brown, Marie Annette; Crabtree, Katherine

    2013-01-01

    Doctoral education in nursing is undergoing a paradigm shift. The doctor of nursing practice (DNP) degree was conceived to improve practice expertise and accelerate the translation of evidence into practice. The need for doctoral preparation that focuses on both expert practice and practice inquiry acknowledges the complexity of evidence-based practice. The DNP prepares graduates to practice at the most advanced level of nursing and to evaluate current practice approaches, to appraise evidence, and to use this knowledge to create clinical strategies that improve practice and health outcomes. DNP curricula need to evolve through shared best practices grounded in financial sustainability. Because of the evolutionary nature of new programs, there is a need for continuous rapid adjustment of the DNP curriculum. Ideas presented here have evolved with student, faculty, and agency input. Many elements of DNP curricula represent a shared world view of faculty across the county. However, consensus about the DNP project has not been reached and is urgently needed to promote acceptance of the DNP-prepared advanced practice registered nurse. The DNP project allows synthesis of the American Association of Colleges of Nursing DNP Essentials through "real world" translation of evidence into practice. This article proposes that DNP projects be defined as practice improvement partnerships between academia and community agencies. This "win-win" collaboration can improve care while preparing the next generation of nurses for the demands of increasingly complex healthcare environments.

  4. Hydroxyapatites: Key Structural Questions and Answers from DNP (Dynamic Nuclear Polarization).

    Science.gov (United States)

    Leroy, Cesar; Aussenac, Fabien; Bonhomme-Coury, Laure; Osaka, Akiyoshi; Hayakawa, Satoshi; Babonneau, Florence; Coelho Diogo, Cristina; Bonhomme, Christian

    2017-09-05

    We demonstrate that NMR/DNP (Dynamic Nuclear Polarization) allows an unprecedented description of carbonate substituted hydroxyapatite (CHAp). Key structural questions related to order/disorder and clustering of carbonates are tackled by using distance sensitive DNP experiments using (13)C-(13)C recoupling. Such experiments are easily implemented due to huge DNP gain (orders of magnitude). DNP is efficiently mediated by quasi one-dimensional spin diffusion through the hydroxyl columns present in the CHAp structure (thought as "highways" for spin diffusion). For spherical nanoparticles (< 100 nm), it is numerically shown that spin diffusion allows their study as a whole. Most importantly, we demonstrate also that the DNP study at 100 K leads to data which are comparable to the one obtained at room temperature (in terms of spin dynamics and lineshape resolution). Finally, all 2D DNP experiments can be interpreted in terms of domains exhibiting well identified types of substitution: local order and carbonate clustering are clearly favored.

  5. A novel vacuum window for megawatt gyrotrons

    Science.gov (United States)

    Haldeman, George Stephenson

    2001-08-01

    This thesis describes a new microwave output window for megawatt power level, 110 GHz gyrotrons. The window uses two spherically polished sapphire elements separated by a nearly uniform gap of about 1 mm. Pressurized, microwave transparent coolant flows through this gap and across the transmitting aperture, removing absorbed energy at the element face. The design differs from previous face- cooled windows because the sapphire elements have a modestly curved dome, rather than flat shape. This curved shape increases the element's capability to withstand coolant pressures without significantly increasing stresses from differential heating. Consequently, window power can be increased either through enhanced, high pressure cooling or by using thinner elements with reduced microwave absorption. A model was developed to predict window power capability as a function of design geometry. This model predicted an increase from 0.5 to 2.1 MW when flat elements were changed to curved. Both designs used full size, 100mm clear apertures, but the curved elements with 214 mm curvature radii were 1.3 rather than 1.75 mm thick. The model was also used to design a half size prototype experiment. This 50 mm clear aperture system used 60 mm OD x 0.47 mm thick x 107 mm surface radius elements. An initial piece was fabricated and statically tested to a pressure of 1.1 MPa well above the 0.5 MPa analytically predicted to be required for megawatt operation. The complete window structure was then fabricated, including instrumentation to simulate microwave heating and to measure induced temperatures and strains. Test results demonstrated an equivalent continuous Gaussian beam power capability of 700 kW using a coolant flow of 1.1 lps. Scaling from this measurement, fall size boiling limited power is expected to be 1.1 MW for a Gaussian microwave intensity profile, or 2.3 MW for a shaped profile typical of previous flat, face-cooled designs. (Copies available exclusively from MIT Libraries, Rm

  6. Recent Tests on 117.5 GHz and 170 GHz Gyrotrons

    Directory of Open Access Journals (Sweden)

    Felch K.

    2015-01-01

    Full Text Available Two megawatt-class gyrotrons at frequencies of 117.5 GHz and 170 GHz have recently been fabricated and tested at CPI. The 117.5 GHz gyrotron was designed to produce up to 1.8 MW for 10-second pulses, and will be used for electron cyclotron heating and current drive on the DIII-D tokamak at General Atomics. The 170 GHz gyrotron is specified as a 500 kW CW system, but has been designed with the goal of generating up to 1 MW CW. Oak Ridge National Laboratory will use the gyrotron in ITER ECH transmission line testing.

  7. Exploring early and future use of DNP prepared nurses within healthcare organizations.

    Science.gov (United States)

    Nichols, Catherine; O'Connor, Nancy; Dunn, Deborah

    2014-02-01

    A strategy to gather data on DNP utilization and impact was designed using Donabedian's conceptual model and was piloted by surveying chief nursing officers (CNOs) leading Michigan's public and teaching hospital systems. Few of the responding CNOs reported currently employing DNP-prepared nurses. The majority reported gaps in knowledge related to role expectations and projected outcomes from a DNP-prepared nurse. Nurse leaders should become familiar with the role competencies of the DNP in order to maximize the potential contribution of this new level of care provider to improving care quality and access.

  8. Herramientas DNP3 pentesting para redes de infraestructura critica

    Directory of Open Access Journals (Sweden)

    M. Sánchez

    2016-07-01

    Full Text Available Este artículo presenta un conjunto de herramientas de software que son capaces de realizar actividades Pentesting en la infraestructura crítica del sector eléctrico mediante el protocolo DNP3. Las herramientas son capaces de comprobar la capacidad de los controles de seguridad cibernética en el interior del perímetro de la red para evitar cualquier comando sensible falsificado pueda llegar a cualquier controlador de subestación

  9. Further studies of a simple gyrotron equation: linear theory

    Energy Technology Data Exchange (ETDEWEB)

    Weitzner, Harold [New York University, Courant Institute of Mathematical Sciences New York, NY 10012 (United States)

    2009-07-03

    A linearized version of a standard system of gyrotron model equations is studied. The linearization allows the inclusion of some effects of particle bunching. The normal modes of the linearized system are given. It is shown that bunching effects couple incoming and outgoing waves. The waves near resonance duplicate well-known results. Without bunching and with a simple background profile function integral representations of solutions are given and discussed.

  10. Investigations and advanced concepts on gyrotron interaction modeling and simulations

    Science.gov (United States)

    Avramidis, K. A.

    2015-12-01

    In gyrotron theory, the interaction between the electron beam and the high frequency electromagnetic field is commonly modeled using the slow variables approach. The slow variables are quantities that vary slowly in time in comparison to the electron cyclotron frequency. They represent the electron momentum and the high frequency field of the resonant TE modes in the gyrotron cavity. For their definition, some reference frequencies need to be introduced. These include the so-called averaging frequency, used to define the slow variable corresponding to the electron momentum, and the carrier frequencies, used to define the slow variables corresponding to the field envelopes of the modes. From the mathematical point of view, the choice of the reference frequencies is, to some extent, arbitrary. However, from the numerical point of view, there are arguments that point toward specific choices, in the sense that these choices are advantageous in terms of simulation speed and accuracy. In this paper, the typical monochromatic gyrotron operation is considered, and the numerical integration of the interaction equations is performed by the trajectory approach, since it is the fastest, and therefore it is the one that is most commonly used. The influence of the choice of the reference frequencies on the interaction simulations is studied using theoretical arguments, as well as numerical simulations. From these investigations, appropriate choices for the values of the reference frequencies are identified. In addition, novel, advanced concepts for the definitions of these frequencies are addressed, and their benefits are demonstrated numerically.

  11. Building skills in organizational and systems changes: a DNP-FNP clinical curriculum.

    Science.gov (United States)

    Hoyle, Christine; Johnson, Gail

    2015-04-13

    DNP-prepared nurse practitioner leaders play a pivotal role in organizational change and quality improvement consistent with the IHI Triple Aim: improving quality of care, health of populations, and reducing cost. A DNP-FNP curriculum is described, designed to build students' leadership competencies for systems change in healthcare settings.

  12. Cutaneous drug toxicity from 2,4-dinitrophenol (DNP): Case report and histological description.

    Science.gov (United States)

    Le, Patricia; Wood, Benjamin; Kumarasinghe, Sujith Prasad

    2015-11-01

    The use of 2,4-dinitrophenol (DNP) has regained popularity as a weight loss aid in the last two decades due to increased marketing to bodybuilders and the increasing availability of this banned substance via the Internet. 2,4-DNP is a drug of narrow therapeutic index and toxicity results in hyperthermia, diaphoresis, tachycardia, tachypnoea and possible cardiac arrest and death. Skin toxicity from 2,4-DNP has not been reported since the 1930s. We report a case of a 21-year-old bodybuilding enthusiast who presented with a toxic exanthem after taking 2,4-DNP, and describe the first skin biopsy findings in a case of 2,4-DNP toxicity.

  13. Possibilities for Continuous Frequency Tuning in Terahertz Gyrotrons with Nontunable Electrodynamic Systems

    Science.gov (United States)

    Bratman, V. L.; Savilov, A. V.; Chang, T. H.

    2016-02-01

    Large ohmic losses in the cavities of terahertz gyrotrons may lead to the overlapping of the axial mode spectra. In a number of gyrotron experiments, this effect has been used to provide a fairly broadband frequency tuning by changing appropriately the operating magnetic field and/or accelerating voltage of the gyrotron. Similar to the systems with nonfixed axial structure of the RF electromagnetic field and low diffraction quality, which are due to weak reflections of the operating wave from the collector end of the electrodynamic system, this changing leads to a monotonic change in the axial index of the operating wave and transition from the gyrotron regime to the gyro-BWO regime. According to a theoretical comparison of these two methods performed on the basis of generalization of self-consistent gyrotron equations with allowance for variations in the axial electron momenta, low-reflection systems can provide a higher efficiency and monotonicity of the frequency tuning.

  14. Correlation of the EPR properties of perchlorotriphenylmethyl radicals and their efficiency as DNP polarizers.

    Science.gov (United States)

    Banerjee, Debamalya; Paniagua, Juan Carlos; Mugnaini, Veronica; Veciana, Jaume; Feintuch, Akiva; Pons, Miquel; Goldfarb, Daniella

    2011-11-01

    Water soluble perchlorinated trityl (PTM) radicals were found to be effective 95 GHz DNP (dynamic nuclear polarization) polarizers in ex situ (dissolution) (13)C DNP (Gabellieri et al., Angew Chem., Int. Ed. 2010, 49, 3360). The degree of the nuclear polarization obtained was reported to be dependent on the position of the chlorine substituents on the trityl skeleton. In addition, on the basis of the DNP frequency sweeps it was suggested that the (13)C NMR signal enhancement is mediated by the Cl nuclei. To understand the DNP mechanism of the PTM radicals we have explored the 95 GHz EPR characteristics of these radicals that are relevant to their performance as DNP polarizers. The EPR spectra of the radicals revealed axially symmetric g-tensors. A comparison of the spectra with the (13)C DNP frequency sweeps showed that although the solid effect mechanism is operational the DNP frequency sweeps reveal some extra width suggesting that contributions from EPR forbidden transitions involving (35,37)Cl nuclear flips are likely. This was substantiated experimentally by ELDOR (electron-electron double resonance) detected NMR measurements, which map the EPR forbidden transitions, and ELDOR experiments that follow the depolarization of the electron spin upon irradiation of the forbidden EPR transitions. DFT (density functional theory) calculations helped to assign the observed transitions and provided the relevant spin Hamiltonian parameters. These results show that the (35,37)Cl hyperfine and nuclear quadrupolar interactions cause a considerable nuclear state mixing at 95 GHz thus facilitating the polarization of the Cl nuclei upon microwave irradiation. Overlap of Cl nuclear frequencies and the (13)C Larmor frequency further facilitates the polarization of the (13)C nuclei by spin diffusion. Calculation of the (13)C DNP frequency sweep based on the Cl nuclear polarization showed that it does lead to an increase in the width of the spectra, improving the agreement with the

  15. High power 303 GHz gyrotron for CTS in LHD

    Science.gov (United States)

    Yamaguchi, Y.; Kasa, J.; Saito, T.; Tatematsu, Y.; Kotera, M.; Kubo, S.; Shimozuma, T.; Tanaka, K.; Nishiura, M.

    2015-10-01

    A high-power pulsed gyrotron is under development for 300 GHz-band collective Thomson scattering (CTS) diagnostics in the Large Helical Device (LHD). High-density plasmas in the LHD require a probe wave with power exceeding 100 kW in the sub-terahertz region to obtain sufficient signal intensity and large scattering angles. At the same time, the frequency bandwidth should be less than several tens of megahertz to protect the CTS receiver using a notch filter against stray radiations. Moreover, duty cycles of ~ 10% are desired for the time domain analysis of the CTS spectrum. At present, a 77 GHz gyrotron for electron cyclotron heating is used as a CTS wave source in the LHD. However, the use of such a low-frequency wave suffers from refraction, cutoff and absorption at the electron cyclotron resonance layer. Additionally, the signal detection is severely affected by background noise from electron cyclotron emission. To resolve those problems, high-power gyrotrons in the 300 GHz range have been developed. In this frequency range, avoiding mode competition is critical to realizing high-power and stable oscillation. A moderately over-moded cavity was investigated to isolate a desired mode from neighbouring modes. After successful tests with a prototype tube, the practical one was constructed with a cavity for TE22,2 operation mode, a triode electron gun forming intense laminar electron beams, and an internal mode convertor. We have experimentally confirmed single mode oscillation of the TE22,2 mode at the frequency of 303.3 GHz. The spectrum peak is sufficiently narrow. The output power of 290 kW has been obtained at the moment.

  16. Communication Test for ‘MatrikonOPC Server for SCADA DNP 3’ with RTDS

    DEFF Research Database (Denmark)

    Wu, Qiuwei; Cha, Seung-Tae; Saleem, Arshad;

    2010-01-01

    The purpose of the communication test for ‘MatrikonOPC server for SCADA DNP 3’ with RTDS is to verify the data exchange between the ‘MatrikonOPC server for SCADA DNP 3’ and the RTDS using the DNP 3 protocol.The communication test is part of the work for the ‘Wind in Øresund’ project. The objective...... of the ‘Wind in Øresund’ project is to build a demonstration and education system of power system operation and control with a RTDS and a SCADA system....

  17. Effects of electron-cyclotron instabilities on gyrotron beam quality

    Energy Technology Data Exchange (ETDEWEB)

    Jost, G.; Tran, T.M.; Appert, K. [Ecole Polytechnique Federale, Lausanne (Switzerland). Centre de Recherche en Physique des Plasma (CRPP); Wuethrich, S. [CRAY Research, PATP/PSE, EPFL, Lausanne (Switzerland)

    1996-02-01

    A two-dimensional PIC code aimed at the investigation of electron-cyclotron beam instabilities in gyrotrons and their effects on the beam quality is presented. The code is based on recently developed techniques for handling charge conservation and open boundaries. It has been implemented on the massively parallel computer CRAY T3D. First results show an electromagnetic backward instability periodically growing and decaying to energy levels close to those obtained from the electrostatic Bernstein wave instability. On the average, the resulting beam degradation is 3 to 4 times larger than that predicted by electrostatic models. (author) 8 figs., 14 refs.

  18. Implementation of DNP3.0 in the EMCC System of Jinan Steel%DNP3.0在济钢EMCC系统中的实现

    Institute of Scientific and Technical Information of China (English)

    田坤

    2014-01-01

    The basic structure and function of DNP3.0 were mainly introduced. The specific method for implementation of DNP protocol in MOX was given taking the energy management and control center of Jinan Steel as an example, to achieve the “four remote”function through the protocol. Operation result has showed that DNP3.0 protocol has high communication efficiency and reliability.%主要介绍DNP3.0的基本结构和功能,结合济钢能源管控中心的实例,给出了DNP协议在MOX中的具体实现方法,通过该协议系统实现了“四遥”功能。现场运行结果显示,DNP3.0协议具有较高的通信效率和通信可靠性。

  19. 140 GHz gyrotron development program. Quarterly report No. 4, January-March 1985

    Energy Technology Data Exchange (ETDEWEB)

    Felch, K.L.; Bier, R.E.; Craig, L.J.; Fox, L.J.; Hu, G.; Huey, H.E.; Ives, R.L.; Jory, H.R.; Lopez, N.C.; Reysner, P.I.

    1986-01-01

    The objective of this program is to develop a gyrotron oscillator capable of producing 100 kW CW at 140 GHz. Further analysis of the electron guns, interaction cavity, and beam tunnel designs for the first two experimental tubes, Experimental Tube 1 and preprototype Tube 1, is presented. A window deflection tester has been built and initial deflection test results are given. The first 140 GHz gyrotron magnet has successfully passed the major points in the acceptance test. The detailed results of the magnet acceptance test are discussed. Progress concerning the fabrication of 149 GHz gyrotron components, diagnostics, and protective devices, as well as the status of Experimental Tube 1, are summarized.

  20. Developing DNP students as adaptive leaders: a key strategy in transforming health care.

    Science.gov (United States)

    Kendall-Gallagher, Deborah; Breslin, Eileen

    2013-01-01

    The success of graduates with a doctor of nursing practice (DNP) degree in transforming health care will depend significantly on their leadership ability to think strategically, innovate, and engage stakeholders in meaningful system improvement. Known as adaptive work, these graduates will need a portfolio of adaptive leadership skills that prepare them to move health care from a volume-driven to value-based system. This article describes development of a core DNP leadership course in a postmaster's point of entry DNP program at an academic health science center school of nursing. The course, designed as DNP students' initial step on their professional development journey to becoming adaptive leaders capable of driving transformative change, created an alternative lens for students to undertake strategic adaptive change initiatives within themselves and their organizations.

  1. Application of Fusion Gyrotrons to Enhanced Geothermal Systems (EGS)

    Science.gov (United States)

    Woskov, P.; Einstein, H.; Oglesby, K.

    2013-10-01

    The potential size of geothermal energy resources is second only to fusion energy. Advances are needed in drilling technology and heat reservoir formation to realize this potential. Millimeter-wave (MMW) gyrotrons and related technologies developed for fusion energy research could contribute to enabling EGS. Directed MMW energy can be used to advance rock penetration capabilities, borehole casing, and fracking. MMWs are ideally suited because they can penetrate through small particulate extraction plumes, can be efficiently guided long distances in borehole dimensions, and continuous megawatt sources are commercially available. Laboratory experiments with a 10 kW, 28 GHz CPI gyrotron have shown that granite rock can be fractured and melted with power intensities of about 1 kW/cm2 and minute exposure times. Observed melted rock MMW emissivity and estimated thermodynamics suggest that penetrating hot, hard crystalline rock formations may be economic with fusion research developed MMW sources. Supported by USDOE, Office of Energy Efficiency and Renewable Energy and Impact Technologies, LLC.

  2. A Two Frequency 1.5 MW Gyrotron Experiment

    Science.gov (United States)

    Tax, David; Guss, William; Shapiro, Michael; Temkin, Richard; Rock, Ben; Vernon, Ronald; Neilson, Jeffrey

    2012-10-01

    Megawatt gyrotrons are an important microwave source for electron cyclotron heating and current drive (ECH/ECCD) in fusion plasmas due to their ability to produce megawatts of power at millimeter wave frequencies. The MIT gyrotron operates nominally at 96 kV and 40 A with 3 μs pulses and has previously demonstrated 1.5 MW of output power with > 50 % efficiency at 110 GHz with a depressed collector. A new cavity has been designed for 1.5 MW operation at two distinct frequencies: 110 GHz in the TE22,6 mode and 124.5 GHz in the TE24,7 mode. A new internal mode converter (IMC) consisting of a dimpled wall launcher and four smooth curved mirrors has also been designed and was optimized for both modes. Simulations of the IMC indicate that > 98 % Gaussian beam content could be achieved for each mode. Cold test results for the components will be presented as well as the current status of the hot test experiment.

  3. Long-Term Operating Experience with High-Power Gyrotron Oscillators

    Science.gov (United States)

    Felch, Kevin

    2005-10-01

    High-power, megawatt-class gyrotron oscillators have now been used in electron cyclotron heating (ECH) experiments for several years. The long periods of sustained operation have provided important information about the design limits that had initially been placed on the key elements of the gyrotron. In particular, observations made on recent 110 GHz, 1 MW gyrotrons used in ECH experiments on DIII-D at General Atomics indicate that several of the important components of the device, including the electron guns, interaction cavities and diamond output windows, have performed quite well, while analyses of the electron beam collectors on some of the devices indicate that design limits have often been exceeded. Observations made on these gyrotrons will be summarized and plans to address problem areas will be discussed.

  4. High Power Microwave Emission of Large and Small Orbit Gyrotron Devices in Rectangular Interaction Structures

    Science.gov (United States)

    Hochman, J. M.; Gilgenbach, R. M.; Jaynes, R. L.; Rintamaki, J. I.; Luginsland, J. W.; Lau, Y. Y.; Spencer, T. A.

    1996-11-01

    Experiments utilize large and small orbit e-beam gyrotron devices in a rectangular-cross-section (RCS) gyrotron. This device is being explored to examine polarization control. Other research issues include pulse shortening, and mode competition. MELBA generates electron beams with parameters of: -800kV, 1-10kA diode current, and 0.5-1.0 μ sec pulselengths. The small orbit gyrotron device is converted to a large orbit experiment by running MELBA's annular electron beam through a magnetic cusp. Initial experiments showed an increase in beam alpha (V_perp/V_par) of a factor of ~ 4 between small and large orbit devices. Experimental results from the RCS gyrotron will be compared for large-orbit and small-orbit electron beams. Beam transport data and frequency measurements will be presented. Computer modeling utilizing the MAGIC and E-gun codes will be shown.

  5. Ion Compensation for Space Charge in the Helical Electron Beams of Gyrotrons

    Science.gov (United States)

    Manuilov, V. N.; Semenov, V. E.

    2016-06-01

    We solve analytically the problem about ion compensation for the space charge of a helical electron beam in a gyrotron operated in the long-pulse regime. Elementary processes, which take place during ionization of residual gas in the tube under typical pressures of 10-6-10-7 mm Hg, are considered. It is shown that distribution of the space charge is affected mainly by the electrons of the initial beam and slow-moving ions produced by ionization of the residual gas. Steady-state density of ions in the operating space of the gyrotron after the end of the transitional processes is found, as well as the electron density profile in the channel of electron beam transportation. The results obtained allow us to evaluate the pitch-factor variations caused by partial compensations for the potential "sagging" in the gyrotron cavity, thus being useful for analysis of starting currents, efficiency, and mode competition in high-power gyrotrons.

  6. Cold test of cylindrical open resonator for 42 GHz, 200 kW gyrotron

    Indian Academy of Sciences (India)

    Vivek Yadav; Sudeep Sharan; Hasina Khatun; Nitin Kumar; M K Alaria; B Jha; S C Deorani; A K Sinha; P K Jain

    2013-12-01

    This paper presents experimental results for cold testing of a gyrotron open resonator. Experiments were carried out to measure resonant frequency and their particular quality factor for TE mode at the frequency 42 GHz. The perturbation technique was used to determine the axial, radial and azimuthal electric field profile for identification of TE031 mode at operating frequency 42 GHz. The good agreement between experimental results and theoretical studies was found. The results verify the design and fabrication of the specific gyrotron cavity.

  7. Optimization of operation of a three-electrode gyrotron with the use of a flow-type calorimeter

    Energy Technology Data Exchange (ETDEWEB)

    Kharchev, Nikolay K.; Batanov, German M.; Kolik, Leonid V.; Malakhov, Dmitrii V.; Petrov, Aleksandr Ye.; Sarksyan, Karen A.; Skvortsova, Nina N.; Stepakhin, Vladimir D. [Prokhorov General Physics Institute, Vavilova ul. 38, Moscow 119991 (Russian Federation); Belousov, Vladimir I. [Institute of Applied Physics, Ul' yanova ul. 46, Nizhnii Novgorod 603950 (Russian Federation); Malygin, Sergei A.; Tai, Yevgenii M. [GYCOM Company, Ul' yanova ul. 46, Nizhnii Novgorod 603155 (Russian Federation)

    2013-01-15

    Results are presented for measurements of microwave power of the Borets-75/0.8 gyrotron with recovery of residual electron energy, which were performed by a flow-type calorimeter. This gyrotron is a part of the ECR plasma heating complex put into operation in 2010 at the L-2M stellarator. The new calorimeter is capable of measuring microwave power up to 0.5 MW. Monitoring of the microwave power makes it possible to control the parameters of the gyrotron power supply unit (its voltage and current) and the magnetic field of the cryomagnet in order to optimize the gyrotron operation and arrive at maximum efficiency.

  8. Millimeter-wave gyrotron traveling-wave tube amplifiers

    CERN Document Server

    Du, Chao-Hai

    2014-01-01

    A gyrotron traveling-wave amplifier (gyro-TWT) with the high-power and broad-band capabilities is considered as a turn-on key for next generation high-resolution radar. The book presents comprehensive theory, methods, and physics related to gyro-TWT. The most challenging problem of instability competition has been for the first time addressed in a focused and systematic way, and reported via concise states and vivid pictures. The book is likely to meet the interest of researchers and engineers in radar and microwave technology, who would like to study the gyro-TWTs and to promote its application in millimeter-wave radars.   Chao-Hai Du is a research professor, and Pu-Kun Liu is a full professor, at Peking University, Beijing, P. R. China.

  9. Operation and control of high power Gyrotrons for ECRH systems in SST-1 and Aditya

    Energy Technology Data Exchange (ETDEWEB)

    Shukla, B.K., E-mail: shukla@ipr.res.in; Bora, D.; Jha, R.; Patel, Jatin; Patel, Harshida; Babu, Rajan; Dhorajiya, Pragnesh; Dalakoti, Shefali; Purohit, Dharmesh

    2016-11-15

    Highlights: • Operation and control of high power Gyrotrons. • Data acquisition and control (DAQ) for Gyrotron system. • Ignitron based crowbar protection. • VME and PXI based systems. - Abstract: The Electron Cyclotron Resonance Heating (ECRH) system is an important heating system for the reliable start-up of tokamak. The 42 GHz and 82.6 GHz ECRH systems are used in tokamaks SST-1 and Aditya to carry out ECRH related experiments. The Gyrotrons are high power microwave tubes used as a source for ECRH systems. The Gyrotron is a delicate microwave tube, which deliver megawatt level power at very high voltage ∼40–50 kV with the current requirement ∼10 A–50 A. The Gyrotrons are associated with the subsystems like: High voltage power supplies (Beam voltage and anode voltage), dedicated crowbar system, magnet, filament and ion pump power supplies, cooling, interlocks and a dedicated data acquisition & control (DAC) system. There are two levels of interlocks used for the protection of Gyrotron: fast interlocks (arcing, beam over current, dI/dt, anode voltage and anode over current etc.) operate within 10 μs and slow interlocks (cooling, filament, silence of Gyrotron, ion pump and magnet currents) operate within 100 ms. Two Gyrotrons (42 GHz/500 kW/500 ms and 82.6 GHz/200 kW/1000 s) have been commissioned on dummy load for full parameters. The 42 GHz ECRH system has been integrated with SST-1 & Aditya tokamak and various experiments have been carried out related to ECRH assisted breakdown and start-up of tokamak at fundamental and second harmonic. These Gyrotrons are operated with VME based data acquisition and control (DAC) system. The DAC system is capable to acquire 64 digital and 32 analog signals. The system is used to monitor & acquire the data and also used for slow interlocks for the protection of Gyrotron. The data acquired from the system are stored online on VME system and after the shot stored in a file in binary format. The MDSPlus, a set of

  10. Faculty and organizational characteristics associated with informatics/health information technology adoption in DNP programs.

    Science.gov (United States)

    Fulton, Cathy R; Meek, Julie A; Walker, Patricia Hinton

    2014-01-01

    Nursing informatics/health information technology are key components of graduate nursing education and an accreditation requirement, yet little is known about the extent to which doctor of nursing practice (DNP) curricula include these content domains. The purpose of this descriptive study was to elicit perceptions of DNP program directors relative to (a) whether and how the American Association of Colleges of Nursing's (AACN's) Essential IV standard has been met in their DNP programs; (b) whether the Technology Informatics Guiding Educational Reform Initiative Foundation's Phase II competencies have been integrated in their programs; and (c) the faculty and organizational characteristics associated with the adoption of the AACN's Essential IV. In 2011, an electronic survey was sent to all 138 DNP program directors identified on the AACN Web site with an 81.2% response rate. Findings include variation in whether and how programs have integrated informatics/health information technology content, a lack of informatics-certified and/or master's-prepared faculty, and a perceived lack of faculty awareness of informatics curricular guidelines. DNP program director and dean awareness and support of faculty informatics education, use of informatics competency guidelines, and national policy and stimulus funding support are recommended to promote curricular inclusion and the engagement of nurses in strong informatics practices.

  11. Identifying Barriers and Facilitators to Future Nurse Faculty Careers for DNP Students.

    Science.gov (United States)

    Fang, Di; Bednash, Geraldine D

    Increasing the pool of doctorally educated nurses pursuing faculty careers is imperative in the development of the nurse faculty workforce. This cross-sectional study aims to identify barriers and facilitators to academic careers for doctor of nursing practice (DNP) students. One thousand five hundred DNP students were randomly selected from nursing schools across the country to participate in our survey, and a 56.9% response rate was achieved. The study found that 32% of respondents planned to pursue faculty careers after graduating. Students with postgraduation plans for academic careers, nonacademic careers, and undecided careers did not show distinct differences in demographic and academic characteristics, except that students who planned to pursue academic careers were more likely to have full-time and part-time faculty status. However, students in the 3 groups perceived facilitators and barriers to academic careers differently. The most influential facilitators were interest in teaching and an appreciation of the impact of nursing research on patient care, and the most considered barriers were poor financial compensation and a negative perception of academia. In terms of academic preparation, a large percentage of DNP students who planned to pursue a faculty career reported that they were not confident in teaching informatics. These findings are also consistent for DNP students who were not a faculty member. The impact of DNP education appeared to have a small, although positive, impact on students' decisions to pursue academic careers. Copyright © 2016 Elsevier Inc. All rights reserved.

  12. Development of dual frequency gyrotron and high power test of EC components

    Directory of Open Access Journals (Sweden)

    Sakamoto K.

    2012-09-01

    Full Text Available In JAEA, development of high-power long-pulse gyrotrons is underway. The output power of the gyrotron was applied for high-power long-pulse tests of the transmission line (TL and the equatorial launcher (EL mock up for ITER. The feature of design in the dual frequency gyrotron is the simultaneously satisfying the matching of both frequencies at a window and the same radiation angle at an internal mode convertor for both frequencies. The dual frequency gyrotron was developed and high power operations at 170 GHz and 137 GHz were carried out. The 170 GHz high power experiment of 40 m length ITER relevant TL was carried out and transmission efficiency and mode purity change caused by long pulse operation were measured. The mock-up model of EL was also tested using 170 GHz gyrotron. The power transmission through the quasi-optical beam line in EL was demonstrated using the full scale mock up model. Furthermore, the high power test results of the transmission components will be summarized.

  13. Millimeter-wave, megawatt gyrotron development for ECR (electron cyclotron resonance) heating applications

    Energy Technology Data Exchange (ETDEWEB)

    Jory, H.; Felch, K.; Hess, C.; Huey, H.; Jongewaard, E.; Neilson, J.; Pendleton, R.; Tsirulnikov, M. (Varian Associates, Inc., Palo Alto, CA (USA))

    1990-09-17

    To address the electron cyclotron heating requirements of planned fusion experiments such as the International Thermonuclear Experimental Reactor (ITER) and the Compact Ignition Tokamak (CIT), Varian is developing gyrotrons at frequencies ranging from 100--300 GHz with output power capabilities up to 1 MW CW. Experimental gyrotrons have been built at frequencies between 100--140 GHz, and a study program has addressed the critical elements of designing 280--300 GHz gyrotrons capable of generating CW power levels up to 1 MW. Initial test vehicles at 140 GHz have utilized TE{sub 15,2,1} interaction cavities, and have been designed to generate short-pulse (up to 20 ms) power levels of 1 MW and up to 400 kW CW. Recently, short-pulse power levels of 1040 kW at 38% efficiency have been obtained and average powers of 200 kW have been achieved. Long-pulse operation has been extended to pulse durations of 0.5 seconds at power levels of 400 kW. Gyrotron oscillators capable of generating output powers of 500 kW CW at a frequency of 110 GHz have recently been designed and a prototype is currently being tested. Design work for a 1 MW CW gyrotron at 110 GHz, is in progress. The 1 MW CW tube will employ an output coupling approach where the microwave output is separated from the microwave output. 15 refs., 10 figs., 3 tabs.

  14. Plasma scattering measurement using a submillimeter wave gyrotron as a radiation source

    Energy Technology Data Exchange (ETDEWEB)

    Ogawa, I.; Idehara, T.; Itakura, Y.; Myodo, M. [Fukui Univ., Research Center for Development of Far-Infrared Region (Japan); Hori, T. [National Institute of Information and Communications Technology, Basic and Advanced Research Division, Nukui-Kita, Koganei (Japan); Hatae, T. [Japan Atomic Energy Research Institute, Mukoyama, Naka (Japan)

    2004-07-01

    Plasma scattering measurement is an effective technique to observe low frequency density fluctuations excited in plasma. The spatial and wave number resolutions and the S/N ratio of measurement depend on the wavelength range, the size and the intensity of a probe beam. A well-collimated, submillimeter wave beam is suitable for improving the spatial and wave number resolutions. Application of high frequency gyrotron is effective in improving the S/N ratio of the measurement because of its capacity to deliver high power. Unlike the molecular vapor lasers, the gyrotrons generate diverging beam of radiation with TE{sub mn} mode structure. It is therefore necessary to convert the output radiation into a Gaussian beam. A quasi-optical antenna is a suitable element for the conversion system under consideration since it is applicable to several TE{sub 0n} and TE{sub 1n} modes. In order to apply the gyrotron to plasma scattering measurement, we have stabilized the output (P = 110 W, f = 354 GHz) of gyrotron up to the level ({delta}P/P < 1 %, {delta}f< 10 kHz). The gyrotron output can be stabilized by decreasing the fluctuation of the cathode potential. (authors)

  15. Communication: Dissolution DNP reveals a long-lived deuterium spin state imbalance in methyl groups

    Science.gov (United States)

    Jhajharia, Aditya; Weber, Emmanuelle M. M.; Kempf, James G.; Abergel, Daniel; Bodenhausen, Geoffrey; Kurzbach, Dennis

    2017-01-01

    We report the generation and observation of long-lived spin states in deuterated methyl groups by dissolution DNP. These states are based on population imbalances between manifolds of spin states corresponding to irreducible representations of the C3v point group and feature strongly dampened quadrupolar relaxation. Their lifetime depends on the activation energies of methyl group rotation. With dissolution DNP, we can reduce the deuterium relaxation rate by a factor up to 20, thereby extending the experimentally available time window. The intrinsic limitation of NMR spectroscopy of quadrupolar spins by short relaxation times can thus be alleviated.

  16. Demonstration of dnp groups on the draining lymph node cells of guinea pigs following skin painting with DNCB by peroxidase labelled antibody method.

    Directory of Open Access Journals (Sweden)

    Tada,Hiroshi

    1981-06-01

    Full Text Available The distribution of 2,4-dinitrophenyl (DNP groups in the draining lymph nodes of guinea pigs 12 h after painting the skin with 2,4-dinitrochlorobenzene (DNCB was examined by a peroxidase labelled antibody method using antibody against DNP groups. DNP groups were detected on cells that were found mainly in the subcapsular sinus of the lymph nodes. Electron microscopic examination showed DNP groups distributed on the surface of lymphocytes. The significance of these findings is discussed.

  17. 1H DNP at 1.4 T of Water Doped with a Triarylmethyl-Based Radical

    DEFF Research Database (Denmark)

    Wind, Robert A.; Ardenkjær-Larsen, Jan Henrik

    1999-01-01

    Recently a triarylmethyl-based (TAM) radical has been developed for research in biological and other aqueous systems, and in low magnetic fields, 10 mT or less, large 1H dynamic nuclear polarization (DNP) enhancements have been reported. In this paper the DNP properties of this radical have been.......85, and the coupling factor is −0.0187. It is shown that these results agree very well with an analysis where the electron–dipolar interactions are the dominant DNP mechanism, and where the relaxation transitions resulting from these interactions are governed by translational diffusion of the water molecules. Finally......, the possibilities of combining DNP with magnetic resonance microscopy (MRM) are discussed. It is shown that at 26°C the overall DNP-enhanced proton polarization should become maximal in an external field of 0.3 T and become comparable to the thermal equilibrium polarization in a field of 30 T, considerably larger...

  18. Design and operation of 140 GHz gyrotron oscillators for power levels up to 1 MW CW

    Energy Technology Data Exchange (ETDEWEB)

    Jory, H.; Bier, R.; Craig, L.J.; Felch, K.; Ives, L.; Lopez, N.; Spang, S.

    1986-12-01

    Varian has designed and tested 140 GHz gyrotron oscillators that have generated output powers of 100 kW CW and 200 kW for 1 ms pulses. Upcoming tubes will be designed to operate at power levels of 200 kW CW and ultimately up to 1 MW CW. The important design considerations which are addressed in the higher power tubes include the design of the electron gun, interaction circuit, and output window. These issues will be discussed and the results of the earlier 140 GHz gyrotron work at Varian will be summarized.

  19. Influence of Reflections on Frequency Tunability and Mode Competition in the Second-Harmonic THz Gyrotron

    Science.gov (United States)

    Khutoryan, Eduard M.; Idehara, Toshitaka; Melnikova, Maria M.; Ryskin, Nikita M.; Dumbrajs, Olgierd

    2017-07-01

    Effect of delayed reflection on operation of a second-harmonic terahertz (THz)-band gyrotron is studied. Theoretical analyses, numerical calculations, and experimental observations for the 0.394-THz Fukui University (FU) and continuous wave (CW) IIB gyrotron are presented. The reflections decrease starting current and expand frequency tunability range owing to excitation of high-order axial modes. They also increase frequency stability, i.e., reduce frequency change due to variation of the magnetic field. In addition, the reflections strongly affect mode competition causing suppress of the second-harmonic mode by the fundamental one and vice versa or, in the case of cooperative mode interaction, mutual power increase.

  20. Magnetic resonance imaging of DNP enhancements in a rotor spinning at the magic angle.

    Science.gov (United States)

    Perras, Frédéric A; Kobayashi, Takeshi; Pruski, Marek

    2016-03-01

    Simulations performed on model, static, samples have shown that the microwave power is non-uniformly distributed in the magic angle spinning (MAS) rotor when using conventional dynamic nuclear polarization (DNP) instrumentation. Here, we applied the stray-field magic angle spinning imaging (STRAFI-MAS) experiment to generate a spatial map of the DNP enhancements in a full rotor, which is spun at a low rate in a commercial DNP-MAS NMR system. Notably, we observed that the enhancement factors produced in the center of the rotor can be twice as large as those produced at the top of the rotor. Surprisingly, we observed that the largest enhancement factors are observed along the axis of the rotor as opposed to against its walls, which are most directly irradiated by the microwave beam. We lastly observed that the distribution of enhancement factors can be moderately improved by degassing the sample and increasing the microwave power. The inclusion of dielectric particles greatly amplifies the enhancement factors throughout the rotor. The STRAFI-MAS approach can provide useful guidance for optimizing the access of microwave power to the sample, and thereby lead to further increases in sensitivity of DNP-MAS NMR.

  1. Evaluating resilience of DNP3-controlled SCADA systems against event buffer flooding

    Energy Technology Data Exchange (ETDEWEB)

    Yan, Guanhua [Los Alamos National Laboratory; Nicol, David M [UNIV OF IL; Jin, Dong [UNIV OF IL

    2010-12-16

    The DNP3 protocol is widely used in SCADA systems (particularly electrical power) as a means of communicating observed sensor state information back to a control center. Typical architectures using DNP3 have a two level hierarchy, where a specialized data aggregator device receives observed state from devices within a local region, and the control center collects the aggregated state from the data aggregator. The DNP3 communication between control center and data aggregator is asynchronous with the DNP3 communication between data aggregator and relays; this leads to the possibility of completely filling a data aggregator's buffer of pending events, when a relay is compromised or spoofed and sends overly many (false) events to the data aggregator. This paper investigates how a real-world SCADA device responds to event buffer flooding. A Discrete-Time Markov Chain (DTMC) model is developed for understanding this. The DTMC model is validated by a Moebius simulation model and data collected on real SCADA testbed.

  2. A 140 GHz pulsed EPR/212 MHz NMR spectrometer for DNP studies

    Science.gov (United States)

    Smith, Albert A.; Corzilius, Björn; Bryant, Jeffrey A.; DeRocher, Ronald; Woskov, Paul P.; Temkin, Richard J.; Griffin, Robert G.

    2012-10-01

    We described a versatile spectrometer designed for the study of dynamic nuclear polarization (DNP) at low temperatures and high fields. The instrument functions both as an NMR spectrometer operating at 212 MHz (1H frequency) with DNP capabilities, and as a pulsed-EPR operating at 140 GHz. A coiled TE011 resonator acts as both an NMR coil and microwave resonator, and a double balanced (1H, 13C) radio frequency circuit greatly stabilizes the NMR performance. A new 140 GHz microwave bridge has also been developed, which utilizes a four-phase network and ELDOR channel at 8.75 GHz, that is then multiplied and mixed to obtain 140 GHz microwave pulses with an output power of 120 mW. Nutation frequencies obtained are as follows: 6 MHz on S = 1/2 electron spins, 100 kHz on 1H, and 50 kHz on 13C. We demonstrate basic EPR, ELDOR, ENDOR, and DNP experiments here. Our solid effect DNP results demonstrate an enhancement of 144 and sensitivity gain of 310 using OX063 trityl at 80 K and an enhancement of 157 and maximum sensitivity gain of 234 using Gd-DOTA at 20 K, which is significantly better performance than previously reported at high fields (⩾3 T).

  3. EC as PIE: five criteria for executing a successful DNP final project.

    Science.gov (United States)

    Waldrop, Julee; Caruso, Diane; Fuchs, Mary Ann; Hypes, Kathe

    2014-01-01

    The goal of doctor of nursing practice (DNP) programs should be to produce nurses that are uniquely prepared to bridge the gap between the discovery of new knowledge and the scholarship of translation, application, and integration of this new knowledge in practice (American Association of Colleges of Nursing [AACN], 2006). However, there is concern over the variability in DNP programs and expected outcomes. The aim of this article is to describe a 5-point system of evaluation to determine whether a DNP final project meets the outcomes of the AACN Essentials of Doctoral Education in Advanced Nursing Practice (2006) in a comprehensive and rigorous way. In brief, the five criteria that must be met are represented by the acronym EC as PIE (E = Enhances; C = Culmination; P = Partnerships; I = Implements; E = Evaluates). Each criterion must be present and come together to form one complete "pie" representing evidence-based practice that is robust and innovative, culminating in a rigorous doctoral level DNP final project. In addition, we provide detailed examples of how these standards are currently being successfully implemented and discuss additional possibilities.

  4. A 140 GHz pulsed EPR/212 MHz NMR spectrometer for DNP studies.

    Science.gov (United States)

    Smith, Albert A; Corzilius, Björn; Bryant, Jeffrey A; DeRocher, Ronald; Woskov, Paul P; Temkin, Richard J; Griffin, Robert G

    2012-10-01

    We described a versatile spectrometer designed for the study of dynamic nuclear polarization (DNP) at low temperatures and high fields. The instrument functions both as an NMR spectrometer operating at 212 MHz ((1)H frequency) with DNP capabilities, and as a pulsed-EPR operating at 140 GHz. A coiled TE(011) resonator acts as both an NMR coil and microwave resonator, and a double balanced ((1)H, (13)C) radio frequency circuit greatly stabilizes the NMR performance. A new 140 GHz microwave bridge has also been developed, which utilizes a four-phase network and ELDOR channel at 8.75 GHz, that is then multiplied and mixed to obtain 140 GHz microwave pulses with an output power of 120 mW. Nutation frequencies obtained are as follows: 6 MHz on S=1/2 electron spins, 100 kHz on (1)H, and 50 kHz on (13)C. We demonstrate basic EPR, ELDOR, ENDOR, and DNP experiments here. Our solid effect DNP results demonstrate an enhancement of 144 and sensitivity gain of 310 using OX063 trityl at 80 K and an enhancement of 157 and maximum sensitivity gain of 234 using Gd-DOTA at 20 K, which is significantly better performance than previously reported at high fields (≥3 T).

  5. Hyperpolarized xenon by d-DNP using the clinical GE SpinLab polarizer system

    DEFF Research Database (Denmark)

    Mariager, Christian Østergaard; Ringgaard, Steffen; Ardenkjær-Larsen, Jan Henrik

    2017-01-01

    Hyperpolarized (HP) 129Xe have been demonstrated as a useful probe for magnetic resonance (MR) lung imaging and show promise for in vivo perfusion imaging and brown adipose tissue characterization. Reports of large polarization enhancements for 129Xe using dynamic nuclearpolarization (DNP) have r...

  6. Design and characterization of a W-band system for modulated DNP experiments.

    Science.gov (United States)

    Guy, Mallory L; Zhu, Lihuang; Ramanathan, Chandrasekhar

    2015-12-01

    Magnetic-field and microwave-frequency modulated DNP experiments have been shown to yield improved enhancements over conventional DNP techniques, and even to shorten polarization build-up times. The resulting increase in signal-to-noise ratios can lead to significantly shorter acquisition times in signal-limited multi-dimensional NMR experiments and pave the way to the study of even smaller sample volumes. In this paper we describe the design and performance of a broadband system for microwave frequency- and amplitude-modulated DNP that has been engineered to minimize both microwave and thermal losses during operation at liquid helium temperatures. The system incorporates a flexible source that can generate arbitrary waveforms at 94GHz with a bandwidth greater than 1GHz, as well as a probe that efficiently transmits the millimeter waves from room temperature outside the magnet to a cryogenic environment inside the magnet. Using a thin-walled brass tube as an overmoded waveguide to transmit a hybrid HE11 mode, it is possible to limit the losses to 1dB across a 2GHz bandwidth. The loss is dominated by the presence of a quartz window used to isolate the waveguide pipe. This performance is comparable to systems with corrugated waveguide or quasi-optical components. The overall excitation bandwidth of the probe is seen to be primarily determined by the final antenna or resonator used to excite the sample and its coupling to the NMR RF coil. Understanding the instrumental limitations imposed on any modulation scheme is key to understanding the observed DNP results and potentially identifying the underlying mechanisms. We demonstrate the utility of our design with a set of triangular frequency-modulated DNP experiments.

  7. Design and characterization of a W-band system for modulated DNP experiments

    Science.gov (United States)

    Guy, Mallory L.; Zhu, Lihuang; Ramanathan, Chandrasekhar

    2015-12-01

    Magnetic-field and microwave-frequency modulated DNP experiments have been shown to yield improved enhancements over conventional DNP techniques, and even to shorten polarization build-up times. The resulting increase in signal-to-noise ratios can lead to significantly shorter acquisition times in signal-limited multi-dimensional NMR experiments and pave the way to the study of even smaller sample volumes. In this paper we describe the design and performance of a broadband system for microwave frequency- and amplitude-modulated DNP that has been engineered to minimize both microwave and thermal losses during operation at liquid helium temperatures. The system incorporates a flexible source that can generate arbitrary waveforms at 94 GHz with a bandwidth greater than 1 GHz, as well as a probe that efficiently transmits the millimeter waves from room temperature outside the magnet to a cryogenic environment inside the magnet. Using a thin-walled brass tube as an overmoded waveguide to transmit a hybrid HE11 mode, it is possible to limit the losses to 1 dB across a 2 GHz bandwidth. The loss is dominated by the presence of a quartz window used to isolate the waveguide pipe. This performance is comparable to systems with corrugated waveguide or quasi-optical components. The overall excitation bandwidth of the probe is seen to be primarily determined by the final antenna or resonator used to excite the sample and its coupling to the NMR RF coil. Understanding the instrumental limitations imposed on any modulation scheme is key to understanding the observed DNP results and potentially identifying the underlying mechanisms. We demonstrate the utility of our design with a set of triangular frequency-modulated DNP experiments.

  8. A Fully-Sealed Carbon-Nanotube Cold-Cathode Terahertz Gyrotron

    Science.gov (United States)

    Yuan, Xuesong; Zhu, Weiwei; Zhang, Yu; Xu, Ningsheng; Yan, Yang; Wu, Jianqiang; Shen, Yan; Chen, Jun; She, Juncong; Deng, Shaozhi

    2016-09-01

    Gigahertz to terahertz radiation sources based on cold-cathode vacuum electron technology are pursued, because its unique characteristics of instant switch-on and power saving are important to military and space applications. Gigahertz gyrotron was reported using carbon nanotube (CNT) cold-cathode. It is reported here in first time that a fully-sealed CNT cold-cathode 0.22 THz-gyrotron is realized, typically with output power of 500 mW. To achieve this, we have studied mechanisms responsible for CNTs growth on curved shape metal surface, field emission from the sidewall of a CNT, and crystallized interface junction between CNT and substrate material. We have obtained uniform growth of CNTs on and direct growth from cone-cylinder stainless-steel electrode surface, and field emission from both tips and sidewalls of CNTs. It is essential for the success of a CNT terahertz gyrotron to have such high quality, high emitting performance CNTs. Also, we have developed a magnetic injection electron gun using CNT cold-cathode to exploit the advantages of such a conventional gun design, so that a large area emitting surface is utilized to deliver large current for electron beam. The results indicate that higher output power and higher radiation frequency terahertz gyrotron may be made using CNT cold-cathode electron gun.

  9. Efficient Low-Voltage Operation of a CW Gyrotron Oscillator at 233 GHz.

    Science.gov (United States)

    Hornstein, Melissa K; Bajaj, Vikram S; Griffin, Robert G; Temkin, Richard J

    2007-02-01

    The gyrotron oscillator is a source of high average power millimeter-wave through terahertz radiation. In this paper, we report low beam power and high-efficiency operation of a tunable gyrotron oscillator at 233 GHz. The low-voltage operating mode provides a path to further miniaturization of the gyrotron through reduction in the size of the electron gun, power supply, collector, and cooling system, which will benefit industrial and scientific applications requiring portability. Detailed studies of low-voltage operation in the TE(2) (,) (3) (,) (1) mode reveal that the mode can be excited with less than 7 W of beam power at 3.5 kV. During CW operation with 3.5-kV beam voltage and 50-mA beam current, the gyrotron generates 12 W of RF power at 233.2 GHz. The EGUN electron optics code describes the low-voltage operation of the electron gun. Using gun-operating parameters derived from EGUN simulations, we show that a linear theory adequately predicts the low experimental starting currents.

  10. Development program for a 200 kW, cw gyrotron. Final report

    Energy Technology Data Exchange (ETDEWEB)

    DeHope, W.J.; Ferguson, P.E.; Hart, S.L.; Matranga, V.A.; Sandoval, J.J.; Schmitt, M.J.; Tancredi, J.J.; Wozniak, M.A.

    1984-02-01

    Development of a millimeter-wave device to produce 200 kW of continuous wave power at 60 GHz is described. The device, a gyrotron oscillator, is intended for electron-cyclotron heating of fusion plasmas. The design philosophy is herein discussed and experimental results, both diagnostic and long pulse, are given.

  11. Design of a second cyclotron harmonic gyrotron oscillator with photonic band-gap cavity

    Energy Technology Data Exchange (ETDEWEB)

    Liu Gaofeng; Chen Xiaoan; Tang Changjian, E-mail: angelchen765@163.com [College of Physical Science and Technology of Sichuan University, Chengdu 610065 (China)

    2011-07-27

    A photonic band-gap cavity (PBGC) gyrotron with a frequency of about 98 GHz is designed. Theoretical analyses and numerical calculations are made for the PBGC operating at fundamental and second cyclotron harmonic with a TE{sub 34} waveguide mode to demonstrate the beam-wave interaction. The results show that mode competition is successfully eliminated in the PBGC using mode selectivity and choosing the appropriate operating parameters. As a result, the second harmonic PBGC gyrotron operating at TE{sub 34} mode achieves a higher output efficiency than that of the fundamental. It is also demonstrated that, in the case of the chosen parameters for TE{sub 34} waveguide mode, the use of PBG structure in the second harmonic gyrotron brings about not only a lower operating B-field but also a weaker mode competition. The results show that the high-order electromagnetic mode can be developed to interact with the high cyclotron harmonic using the selectivity of the PBGC, which gives an encouraging outlook for the development of high-harmonic gyrotrons.

  12. Hiring Intentions of Directors of Nursing Programs Related to DNP- and PhD-Prepared Faculty and Roles of Faculty.

    Science.gov (United States)

    Oermann, Marilyn H; Lynn, Mary R; Agger, Charlotte A

    2016-01-01

    This study surveyed administrators of associate degree in nursing (ADN) and bachelor of science in nursing (BSN) programs across the United States to identify hiring intentions and describe the roles and responsibilities of DNP- and PhD-prepared faculty members. The final sample included 253 ADN and 229 BSN programs. ADN programs were neither intentionally hiring nor looking to hire doctorally prepared nurse faculty. Deans and directors of BSN programs reported an average of 3 openings for the next academic year, 2 projected for new PhD-prepared faculty and 1 for a faculty member with a DNP. Schools have made varying decisions regarding the type of appointment (tenure or nontenure track) for DNP-prepared faculty members. Challenges that DNP-prepared faculty members encountered in meeting the role and promotion expectations in their schools focused predominantly on scholarship.

  13. Effects of DNP on the cell surface properties of marine bacteria and its implication for adhesion to surfaces

    Digital Repository Service at National Institute of Oceanography (India)

    Jain, A.; Nishad, K.K.; Bhosle, N.B.

    The effect of 2, 4-dinitrophenol (DNP) on extracelluar polysaccharides (EPS), cell surface charge, and hydrophobicity of six marine bacterial cultures was studied, and its influence on attachment of these bacteria to glass and polystyrene...

  14. Enhancement of cadmium bioremediation by endophytic bacterium Bacillus sp. L14 using industrially used metabolic inhibitors (DCC or DNP)

    Energy Technology Data Exchange (ETDEWEB)

    Luo Shenglian, E-mail: sllou@hnu.cn [College of Environmental Science and Engineering, Hunan University, Changsha 410082 (China); Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082 (China); State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082 (China); Key Laboratory of Jiangxi Province for Ecological Diagnosis-Remediation and Pollution Control, Nanchang 330063 (China); Xiao Xiao [College of Environmental Science and Engineering, Hunan University, Changsha 410082 (China); Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082 (China); Xi Qiang [State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082 (China); Wan Yong; Chen Liang; Zeng Guangming [College of Environmental Science and Engineering, Hunan University, Changsha 410082 (China); Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082 (China); Liu Chengbin [State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082 (China); Guo Hanjun; Chen Jueliang [College of Environmental Science and Engineering, Hunan University, Changsha 410082 (China); Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082 (China)

    2011-06-15

    Bioremediations of cadmium by endophytic bacterium (EB) L14 (Bacillus sp.) in the presence of industrially used metabolic inhibitors (DCC or DNP) were investigated. In the presence of DCC or DNP, the biomass population of EB L14 was greatly inhibited. However, the cadmium removal of EB L14 increased from 73.6% (in the absence of DCC or DNP) to 93.7% and 80.8%, respectively. The analysis of total and intracellular cadmium concentrations during 24 h of incubation indicated that this enhanced cadmium removal was the inhibition effect of DCC or DNP on the cations export resistance system of EB L14. This unique property strongly indicated the superiority of this endophyte for practical application in cadmium bioremediation in the presence of industrially used metabolic inhibitors.

  15. A DNP nurse-managed Hepatitis C Clinic, improving quality of life for those in a rural area

    National Research Council Canada - National Science Library

    Krebbeks, Virginia P; Cunningham, Vivian M

    2013-01-01

    .... Improving access to care through the development of a Doctor of Nursing Practice (DNP) nurse-managed clinic in a rural setting will improve health outcomes and quality of life for those treated outside the traditional setting...

  16. EPR and DNP Properties of Certain Novel Single Electron Contrast Agents Intended for Oximetric Imaging

    DEFF Research Database (Denmark)

    Ardenkjær-Larsen, J. H.; Laursen, I; Leunbach, I.;

    1998-01-01

    Parameters of relevance to oximetry with Overhauser magnetic resonance imaging (OMRI) have been measured for three single electron contrast agents of the triphenylmethyl type. The single electron contrast agents are stable and water soluble. Magnetic resonance properties of the agents have been...... examined with electron paramagnetic resonance (EPR), nuclear magnetic resonance (NMR), and dynamic nuclear polarization (DNP) at 9.5 mT in water, isotonic saline, plasma, and blood at 23 and 37°C. The relaxivities of the agents are about 0.2–0.4 mM−1s−1and the DNP enhancements extrapolate close...... to the dipolar limit. The agents have a single, narrow EPR line, which is analyzed as a Voigt function. The linewidth is measured as a function of the agent concentration and the oxygen concentration. The concentration broadenings are about 1–3 μT/mM and the Lorentzian linewidths at infinite dilution are less...

  17. Effect of 6-ketocholestanol on FCCP- and DNP-induced uncoupling in plant mitochondria.

    Science.gov (United States)

    Vianello, A; Macri, F; Braidot, E; Mokhova, E N

    1995-05-22

    Effect of 6-ketocholestanol on FCCP-induced and DNP-induced uncoupling in beef liver and pea stem mitochondria was studied, under experimental conditions at which this steroid abolished the effect of low concentrations of FCCP and other most potent uncouplers in rat mitochondria [Starkov et al. (1994) FEBS Lett., 355, 305-308]. It is shown that, in both types of mitochondria, 6-ketocholestanol prevents or reverses the uncoupling induced by low concentrations of FCCP, but not that caused by high concentrations of FCCP or by any concentration of DNP. Progesterone and male sex hormones, showing recoupling capability in animal mitochondria, appear to be ineffective in the plant system. Cholesterol does not recouple in both animal and plant mitochondria. Plant steroids, such as beta-sitosterol and stigmasterol, are also without effect.

  18. Using problem-based learning to teach health policy at the DNP level.

    Science.gov (United States)

    Hayne, Arlene N; Schlosser, Sharron P

    2014-01-01

    Nursing education recognizes the importance of graduate nurses understanding their roles as advocates, developers, implementers, and evaluators of health policies to impact the delivery of safe, patient-centered care. This article describes the use of problem-based learning as one approach to DNP health policy education. Students select a health problem and an associated health policy and use evidence-based practice, scholarly writing, and health services research to analyze the policy for efficiency, effectiveness, and equity.

  19. Water accessibility in a membrane-inserting peptide comparing Overhauser DNP and pulse EPR methods

    Science.gov (United States)

    Segawa, Takuya F.; Doppelbauer, Maximilian; Garbuio, Luca; Doll, Andrin; Polyhach, Yevhen O.; Jeschke, Gunnar

    2016-05-01

    Water accessibility is a key parameter for the understanding of the structure of biomolecules, especially membrane proteins. Several experimental techniques based on the combination of electron paramagnetic resonance (EPR) spectroscopy with site-directed spin labeling are currently available. Among those, we compare relaxation time measurements and electron spin echo envelope modulation (ESEEM) experiments using pulse EPR with Overhauser dynamic nuclear polarization (DNP) at X-band frequency and a magnetic field of 0.33 T. Overhauser DNP transfers the electron spin polarization to nuclear spins via cross-relaxation. The change in the intensity of the 1H NMR spectrum of H2O at a Larmor frequency of 14 MHz under a continuous-wave microwave irradiation of the nitroxide spin label contains information on the water accessibility of the labeled site. As a model system for a membrane protein, we use the hydrophobic α-helical peptide WALP23 in unilamellar liposomes of DOPC. Water accessibility measurements with all techniques are conducted for eight peptides with different spin label positions and low radical concentrations (10-20 μM). Consistently in all experiments, the water accessibility appears to be very low, even for labels positioned near the end of the helix. The best profile is obtained by Overhauser DNP, which is the only technique that succeeds in discriminating neighboring positions in WALP23. Since the concentration of the spin-labeled peptides varied, we normalized the DNP parameter ɛ, being the relative change of the NMR intensity, by the electron spin concentration, which was determined from a continuous-wave EPR spectrum.

  20. Effects of electron beam parameters and velocity spread on radio frequency output of a photonic band gap cavity gyrotron oscillator

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Ashutosh, E-mail: asingh.rs.ece@iitbhu.ac.in [Faculty of Physical Sciences, Institute of Natural Sciences and Humanities, Shri Ramswaroop Memorial University, Lucknow-Deva Road, Uttar Pradesh 225003 (India); Center of Research in Microwave Tubes, Department of Electronics Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005 (India); Jain, P. K. [Center of Research in Microwave Tubes, Department of Electronics Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005 (India)

    2015-09-15

    In this paper, the effects of electron beam parameters and velocity spread on the RF behavior of a metallic photonic band gap (PBG) cavity gyrotron operating at 35 GHz with TE{sub 041}–like mode have been theoretically demonstrated. PBG cavity is used here to achieve a single mode operation of the overmoded cavity. The nonlinear time-dependent multimode analysis has been used to observe the beam-wave interaction behavior of the PBG cavity gyrotron, and a commercially available PIC code “CST Particle Studio” has been reconfigured to obtain 3D simulation results in order to validate the analytical values. The output power for this typical PBG gyrotron has been obtained ∼108 kW with ∼15.5% efficiency in a well confined TE{sub 041}–like mode, while all other competing modes have significantly low values of power output. The output power and efficiency of a gyrotron depend highly on the electron beam parameters and velocity spread. The influence of several electron beam parameters, e.g., beam voltage, beam current, beam velocity pitch factor, and DC magnetic field, on the PBG gyrotron operations has been investigated. This study would be helpful in optimising the electron beam parameters and estimating accurate RF output power of the high frequency PBG cavity based gyrotron oscillators.

  1. Carbon and proton Overhauser DNP from MD simulations and ab initio calculations: TEMPOL in acetone.

    Science.gov (United States)

    Küçük, Sami Emre; Biktagirov, Timur; Sezer, Deniz

    2015-10-14

    A computational analysis of the Overhauser effect is reported for the proton, methyl carbon, and carbonyl carbon nuclei of liquid acetone doped with the nitroxide radical TEMPOL. A practical methodology for calculating the dynamic nuclear polarization (DNP) coupling factors by accounting for both dipole-dipole and Fermi-contact interactions is presented. The contribution to the dipolar spectral density function of nuclear spins that are not too far from TEMPOL is computed through classical molecular dynamics (MD) simulations, whereas the contribution of distant spins is included analytically. Fermi contacts are obtained by subjecting a few molecules from every MD snapshot to ab initio quantum mechanical calculations. Scalar interaction is found to be an essential part of the (13)C Overhauser DNP. While mostly detrimental to the carbonyl carbon of acetone it is predicted to result in large enhancements of the methyl carbon signal at magnetic fields of 9 T and beyond. In contrast, scalar coupling is shown to be negligible for the protons of acetone. The additional influence of proton polarization on the carbon DNP (three-spin effect) is also analyzed computationally. Its effect, however, is concluded to be practically insignificant for liquid acetone.

  2. Design and characterization of a W-band system for modulated DNP experiments

    CERN Document Server

    Guy, Mallory L; Ramanathan, Chandrasekhar

    2015-01-01

    Magnetic-field and microwave-frequency modulated DNP experiments have been shown to yield improved enhancements over conventional DNP techniques, and even to shorten polarization build-up times. The resulting increase in signal-to-noise ratios can lead to significantly shorter acquisition times in signal-limited multi-dimensional NMR experiments and pave the way to the study of even smaller sample volumes. In this paper we describe the design and performance of a broadband system for microwave frequency- and amplitude-modulated DNP that has been engineered to minimize both microwave and thermal losses during operation at liquid helium temperatures. The system incorporates a flexible source that can generate arbitrary waveforms at 94 GHz with a bandwidth greater than 1 GHz, as well as a probe that efficiently transmits the millimeter waves from room temperature outside the magnet to a cryogenic environment inside the magnet. Using a thin-walled brass tube as an overmoded waveguide to transmit a hybrid HE11 mod...

  3. Status of the development of the EU 170 GHz/1 MW/CW gyrotron

    Energy Technology Data Exchange (ETDEWEB)

    Pagonakis, Ioannis Gr., E-mail: ioannis.pagonakis@kit.edu [Karlsruhe Institute of Technology (KIT), Karlsruhe (Germany); Albajar, Ferran [The European Joint Undertaking for ITER and The Development of Fusion Energy, Barcelona (Spain); Alberti, Stefano [École Polytechnique Fédérale de Lausanne, Centre de Recherches en Physique des Plasmas (CRPP), Lausanne (Switzerland); Avramidis, Konstantinos [Karlsruhe Institute of Technology (KIT), Karlsruhe (Germany); Bonicelli, Tullio [The European Joint Undertaking for ITER and The Development of Fusion Energy, Barcelona (Spain); Braunmueller, Falk [École Polytechnique Fédérale de Lausanne, Centre de Recherches en Physique des Plasmas (CRPP), Lausanne (Switzerland); Bruschi, Alex [Plasma Physics Institute, National Research Council of Italy, Milano (Italy); Chelis, Ioannis [School of Electrical and Computer Engineering, National Technical University of Athens (Greece); Cismondi, Fabio [The European Joint Undertaking for ITER and The Development of Fusion Energy, Barcelona (Spain); Gantenbein, Gerd [Karlsruhe Institute of Technology (KIT), Karlsruhe (Germany); Hermann, Virgile [Thales Electron Devices (TED), Vélizy-Villacoublay (France); Hesch, Klaus [Karlsruhe Institute of Technology (KIT), Karlsruhe (Germany); Hogge, Jean-Philippe [École Polytechnique Fédérale de Lausanne, Centre de Recherches en Physique des Plasmas (CRPP), Lausanne (Switzerland); Jelonnek, John; Jin, Jianbo; Illy, Stefan [Karlsruhe Institute of Technology (KIT), Karlsruhe (Germany); Ioannidis, Zisis C. [Faculty of Physics, National and Kapodistrian University of Athens (Greece); Kobarg, Thorsten [Karlsruhe Institute of Technology (KIT), Karlsruhe (Germany); and others

    2015-10-15

    The progress in the development of the European 170 GHz, 1 MW/CW gyrotron for electron cyclotron heating & current drive (ECH&CD) on ITER is reported. A continuous wave (CW) prototype is being manufactured by Thales Electron Devices (TED), France, while a short-pulse (SP) prototype gyrotron is in parallel under manufacture at Karlsruhe Institute of Technology (KIT), with the purpose of validating the design of the CW industrial prototype components. The fabrication of most of the sub-assemblies of the SP prototype has been completed. In a first step, an existing magnetron injection gun (MIG) available at KIT was used. Despite this non-ideal configuration, the experiments provided a validation of the design, substantiated by an excellent agreement with numerical simulations. The tube, operated without a depressed collector, is able to produce more than 1 MW of output power with efficiency in excess of 30%, as expected, and compatible with the ITER requirements.

  4. Development and simulation of RF components for high power millimeter wave gyrotrons

    Energy Technology Data Exchange (ETDEWEB)

    Pereyaslavets, M.; Sato, M.; Shimozuma, T.; Takita, Y.; Idei, H.; Kubo, S.; Ohkubo, K.; Hayashi, K.

    1996-11-01

    To test gyrotron RF components, efficient low-power generators for rotating high-order modes of high purity are necessary. Designs of generators for the TE{sub 15,3} mode at 84 GHz and for the TE{sub 31,8} mode at 168 GHz are presented and some preliminary test results are discussed. In addition, Toshiba gyrotron cavities at 168 GHz were analyzed for leakage of RF power in the beam tunnel. To decrease RF power leakage, the declination angle of the cut-off cavity cross section has to be decreased. A TE{sub 15,3} waveguide nonlinear uptaper is analyzed at 84 GHz as well as 168 GHz uptapers. Since the calculated conversion losses are slightly higher than designed value, an optimization of those uptapers may be required. (author)

  5. 太赫兹回旋管研究进展%Development of Terahertz Gyrotrons

    Institute of Scientific and Technical Information of China (English)

    鄢扬; 傅文杰

    2013-01-01

    回旋管是一种基于电子回旋谐振受激辐射的快波器件,是目前太赫兹波段输出功率和效率最高的重要器件,本文给出了目前国内外太赫兹回旋管技术的发展状况,分析了太赫兹回旋管的特点和应用前景.%Gyrotrons are fast-wave vacuum electron devices based on the stimulated radiation, of electron cyclotron resonance. They are able to deliver powerful radiation in terahertz frequency bands with high efficiency. In this paper, the current status and development of terahertz gyrotrons are reviewed, the characteristics and prospective applications are discussed.

  6. Gyrotrons for High-Power Terahertz Science and Technology at FIR UF

    Science.gov (United States)

    Idehara, Toshitaka; Sabchevski, Svilen Petrov

    2016-10-01

    In this review paper, we present the recent progress in the development of a series of gyrotrons at the Research Center for Development of Far-Infrared Region, University of Fukui, that have opened the road to many novel applications in the high-power terahertz science and technology. The current status of the research in this actively developing field is illustrated by the most representative examples in which the developed gyrotrons are used as powerful and frequency-tunable sources of coherent radiation operating in a continuous-wave regime. Among them are high-precision spectroscopic techniques (most notably dynamic nuclear polarization-nuclear magnetic resonance, electron spin resonance, X-ray detected magnetic resonance, and studies of the hyperfine splitting of the energy levels of positronium), treatment and characterization of advanced materials, and new medical technologies.

  7. Problem-Oriented Simulation Packages and Computational Infrastructure for Numerical Studies of Powerful Gyrotrons

    Science.gov (United States)

    Damyanova, M.; Sabchevski, S.; Zhelyazkov, I.; Vasileva, E.; Balabanova, E.; Dankov, P.; Malinov, P.

    2016-05-01

    Powerful gyrotrons are necessary as sources of strong microwaves for electron cyclotron resonance heating (ECRH) and electron cyclotron current drive (ECCD) of magnetically confined plasmas in various reactors (most notably ITER) for controlled thermonuclear fusion. Adequate physical models and efficient problem-oriented software packages are essential tools for numerical studies, analysis, optimization and computer-aided design (CAD) of such high-performance gyrotrons operating in a CW mode and delivering output power of the order of 1-2 MW. In this report we present the current status of our simulation tools (physical models, numerical codes, pre- and post-processing programs, etc.) as well as the computational infrastructure on which they are being developed, maintained and executed.

  8. Gyrotrons for High-Power Terahertz Science and Technology at FIR UF

    Science.gov (United States)

    Idehara, Toshitaka; Sabchevski, Svilen Petrov

    2017-01-01

    In this review paper, we present the recent progress in the development of a series of gyrotrons at the Research Center for Development of Far-Infrared Region, University of Fukui, that have opened the road to many novel applications in the high-power terahertz science and technology. The current status of the research in this actively developing field is illustrated by the most representative examples in which the developed gyrotrons are used as powerful and frequency-tunable sources of coherent radiation operating in a continuous-wave regime. Among them are high-precision spectroscopic techniques (most notably dynamic nuclear polarization-nuclear magnetic resonance, electron spin resonance, X-ray detected magnetic resonance, and studies of the hyperfine splitting of the energy levels of positronium), treatment and characterization of advanced materials, and new medical technologies.

  9. Upgrade of a 30 kV/10 mA anode power supply for triode type gyrotron

    Energy Technology Data Exchange (ETDEWEB)

    Siravo, Ugo, E-mail: ugo.siravo@epfl.ch; Alberti, Stefano; Dubray, Jérémie; Fasel, Damien; Hogge, Jean-Philippe; Marlétaz, Blaise; Marmillod, Philippe; Perez, Albert; Silva, Miguel

    2015-10-15

    Highlights: • Triode type gyrotron is fed by 3 power supplies: the main, an anode PS(APS) and a heater. • This paper presents the upgrade of 3APS, supplied in 1999, that never fulfilled the specs. • The new working principle is very efficient, easy to implement, for a minimal cost. • Upgraded APS provides extended modulation capabilities, no overshoot and lowerripple. • This upgrade will allow exploring new operation regimes for the 3 TCV X3 gyrotrons. - The RF power of a gyrotron with a triode type magnetron-injection-gun (MIG) can be directly controlled via the voltage applied between its anode and its cathode. Hence, the performance of this type of gyrotron relies directly on the possibilities offered by the power supply controlling the anode to cathode voltage. For a system of gyrotrons connected to the same main high-voltage power supply, with a triode MIG one has the additional advantage of independently controlling each individual gyrotron. This paper presents the modifications brought to the three existing 30 kV/10 mA anode power supplies connected to the 500 kW/118 GHz/2s X3 gyrotrons operated on the TCV Tokamak. The new working principle based on phase-shift modulation (PSM) is described in detail. Experimental results obtained on dummy load are compared to simulations performed during the design phase. With respect to the initial working principle, the modulation frequency capability has been increased by a factor 10 reaching more than 5 kHz, whereas the output voltage ripple as well as the overshoot/undershoot have been significantly reduced.

  10. A 0.33-THz second-harmonic frequency-tunable gyrotron

    Science.gov (United States)

    Zheng-Di, Li; Chao-Hai, Du; Xiang-Bo, Qi; Li, Luo; Pu-Kun, Liu

    2016-02-01

    Dynamics of the axial mode transition process in a 0.33-THz second-harmonic gyrotron is investigated to reveal the physical mechanism of realizing broadband frequency tuning in an open cavity circuit. A new interaction mechanism about propagating waves, featured by wave competition and wave cooperation, is presented and provides a new insight into the beam-wave interaction. The two different features revealed in the two different operation regions of low-order axial modes (LOAMs) and high-order axial modes (HOAMs) respectively determine the characteristic of the overall performance of the device essentially. The device performance is obtained by the simulation based on the time-domain nonlinear theory and shows that using a 12-kV/150-mA electron beam and TE-3,4 mode, the second harmonic gyrotron can generate terahertz radiations with frequency-tuning ranges of about 0.85 GHz and 0.60 GHz via magnetic field and beam voltage tuning, respectively. Additionally, some non-stationary phenomena in the mode startup process are also analyzed. The investigation in this paper presents guidance for future developing high-performance frequency-tunable gyrotrons toward terahertz applications. Project supported by the National Natural Science Foundation of China (Grant Nos. 61471007, 61531002, 61522101, and 11275206) and the Seeding Grant for Medicine and Information Science of Peking University, China (Grant No. 2014-MI-01).

  11. Experimental Study Of A 1.5-mw, 110-ghz Gyrotron Oscillator

    CERN Document Server

    Anderson, J P

    2005-01-01

    This thesis reports the design, construction and testing of a 1.5 MW, 110 GHz gyrotron oscillator. This high power microwave tube has been proposed as the next evolutionary step for gyrotrons used to provide electron cyclotron heating required in fusion devices. A short pulse gyrotron based on the industrial tube design was built at MIT for experimental studies. The experiments are the first demonstration of such high powers at 110 GHz. Using a 96 kV, 40 A electron beam, over 1.4 MW was axially extracted in the design (TE22,6) mode in 3 μs pulses, corresponding to a microwave efficiency of 37%. The beam alpha, the ratio of transverse to axial velocity in the electron beam, was measured with a probe. At the high efficiency operating point the beam alpha was measured as 1.33. This value of alpha is less than the design value of 1.4, possibly accounting for the slightly reduced experimental efficiency. The output power and efficiency, as a function of magnetic field, beam voltage, and beam current, are in...

  12. Microwave method for synthesis of micro- and nanostructures with controllable composition during gyrotron discharge

    Science.gov (United States)

    Batanov, German M.; Borzosekov, Valentin D.; Golberg, Dmitri; Iskhakova, Ludmila D.; Kolik, Leonid V.; Konchekov, Evgeny M.; Kharchev, Nikolai K.; Letunov, Alexander A.; Malakhov, Dmitry V.; Milovich, Filipp O.; Obraztsova, Ekaterina A.; Petrov, Alexander E.; Ryabikina, Irina G.; Sarksian, Karen A.; Stepakhin, Vladimir D.; Skvortsova, Nina N.

    2016-01-01

    We introduce an approach toward the synthesis of micro- and nanostructures under nonequilibrium microwave discharges within metal-dielectric powder mixtures induced by powerful microwave gyrotron radiation. A new plasma-chemical reactor capable of sustaining a discharge regime with an afterglow phase of an order of magnitude longer than the gyrotron pulse duration was constructed for these experiments. In the nonequilibrium conditions of such a discharge, plasma-induced exothermic chemical reactions leading to the synthesis of various compounds were initiated. The synthesized structures were deposited on the reactor walls and on the impurity particles within the reactor. This method was tested under gyrotron-initiated discharges within various metal-dielectric powder mixtures of titanium-boron, molybdenum-boron, titanium-silicon-boron, molybdenum-boron nitride, molybdenum-tungsten-boron nitride, and so on. Depending on the powder mixture composition, reactor atmosphere, and other parameters, micro- and nanosized particles of boron nitride, titanium diboride, molybdenum boride, titanium boride, molybdenum, and molybdenum oxide, were synthesized, detected, and analyzed.

  13. Commissioning results of the 0.5 MW/68 GHz/1.0 s gyrotron on HL-2A electron cyclotron resonance heating system

    Energy Technology Data Exchange (ETDEWEB)

    Wang, He, E-mail: wangh@swip.ac.cn; Rao, Jun; Huang, Mei; Zhou, Jun; Wang, Chao; Kang, Zihua; Wang, Mingwei; Feng, Kun; Chen, Gangyu; Lu, Bo; Wang, Jieqiong

    2015-12-15

    Highlights: • A test stand for the test of gyrotron is introduced. • Test results show that the output power of gyrotron has a close relation to the high voltage, magnet current and heater power. • The measurement method of operation frequency and output power of gyrotron is introduced. - Abstract: To obtain the optimum operation status of gyrotrons, it is necessary to commissioning the gyrotrons before EC wave injected into plasma for ECRH system on HL-2A tokamak. A test stand has been set up for conditioning the 0.5 MW/68 GHz/1.0 s gyrotrons. The specific test results of gyrotrons are described and discussed in this paper, which include measuring the beam profile by using burn paper at the outlet of MOU and calorimetric dummy load, testing the maximum output power and pulse width on dummy load and getting the dependence between output power and magnetic field current, filament power and high voltage powers. Meanwhile, the operation frequency of gyrotron is also measured in the test.

  14. Experimental investigations of DNP3 protocol for an information embedded power system

    Energy Technology Data Exchange (ETDEWEB)

    Amanullah, M.T.O.; Kalam, A.; Zayegh, A. [Victoria Univ. of Technology, Melbourne (Australia). School of Electrical Engineering

    2007-07-01

    As technology changes at an exponential rate, interconnected bulk electric systems are becoming integrated with vast networked information systems. Utilities must be flexible to cope with changing requirements and technologies while ensuring uninterrupted power supply and other essential features such as interoperability between devices from different suppliers, free allocation of functions and ease of maintenance. Competent power system monitoring and protection is also vital. The power industry spends billions of dollars to transmit its critical data more efficiently and securely. Wide area based Information Embedded Power System (IEPS-W) will provide a robust power system communication infrastructure in order to effectively monitor power system in a larger geographical area. To achieve such a capable communication platform, power systems use a range of power system communication protocols to transmit critical data through Supervisory Control and Data Acquisition (SCADA) systems. A flexible system is needed so that Remote Terminal Units (RTUs) can record power system measurements and transmit them in real time over a Wide Area Network (WAN) to the control centre. In order to study the performance of the Distributed Network Protocol (DNP3) communication protocol over a WAN, an experimental analysis was performed at the Victorian Network Switching Centre owned by SP AusNet of Australia. The experiment revealed a that high propagation delay exists which could cause the entire power network to be uncontrollable. An efficient IEPS system was therefore developed based on the DNP3 protocol using the OPNET modeller. Preliminary simulations have shown promising results for the enhanced DNP3 protocol. 4 refs., 5 figs.

  15. Experimental Research on a 1.5 MW, 110 GHz Gyrotron with a Smooth Mirror Internal Mode Converter

    Science.gov (United States)

    Tax, D. S.; Mastovsky, I.; Shapiro, M. A.; Temkin, R. J.; Torrezan, A. C.

    2010-11-01

    Megawatt gyrotrons are important for electron cyclotron heating (ECH) of fusion plasmas, including ITER. These gyrotrons should operate with high efficiency to reduce the prime power required and to ensure good reliability. The gyrotron efficiency is affected both by the physical principles that govern the device and the performance of components like the internal mode converter (IMC), which must convert the electromagnetic cavity mode into a Gaussian beam. An IMC consisting of a helically-cut launcher and three smooth curved mirrors, which is less susceptible to alignment errors than an IMC using mirrors with phase correcting surfaces, was recently tested on a 1.5 MW, 110 GHz, 3μs pulsed gyrotron operating in the TE22,6 mode, and an output beam with 95.8 ± 0.5 % Gaussian beam content was measured in both hot and cold tests. We are also examining the issue of mode competition in the gyrotron, which can limit the achievable output power and efficiency. The sequence of competing modes excited during the rise time of the voltage pulse has been measured and results are compared with the numerical simulation code MAGY. These results should provide a good test of the accuracy of the code.

  16. A double-beam magnetron-injection gun for third-harmonic continuous wave 1-THz gyrotron

    Energy Technology Data Exchange (ETDEWEB)

    Glyavin, M. [Faculty of Radiophysics, N. I. Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950 (Russian Federation); Institute of Applied Physics of the Russian Academy of Sciences (IAP RAS), Nizhny Novgorod 603600 (Russian Federation); Research Center for Development of Far Infrared Region, University of Fukui (FIR FU), Fukui-shi 910-8507 (Japan); Manuilov, V. [Faculty of Radiophysics, N. I. Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950 (Russian Federation); Research Center for Development of Far Infrared Region, University of Fukui (FIR FU), Fukui-shi 910-8507 (Japan); Idehara, T. [Research Center for Development of Far Infrared Region, University of Fukui (FIR FU), Fukui-shi 910-8507 (Japan)

    2013-12-15

    The concept of a continuous wave 1-kW/1-THz gyrotron operated at the third cyclotron harmonic of the transverse electric TE{sub 9,7} operating mode has been developed. To suppress the mode competition effects in a terahertz gyrotron, we propose a scheme with two generating helical electron beams (HEBs) formed in a double-beam triode magnetron-injection gun (MIG), where both emitters of the electron beams are located on a common cathode of the conventional MIG. An optimal geometry of the MIG electrodes is found. It is shown that in a proposed scheme two HEBs having close pitch factors and a moderate velocity spread can be formed. This makes them suitable for high-efficiency single-mode generation in the high frequency gyrotron at high harmonic.

  17. Design of the all solid high-voltage power supply for a gyrotron body

    Energy Technology Data Exchange (ETDEWEB)

    Rao, Yihua [School of Mathematics and Physics, University of South China, Hengyang, 421001 (China); Chen, Wenguang, E-mail: 430000485393@usc.edu.cn [School of Electrical Engineering, University of South China, Hengyang, 421001 (China); Hu, Bo [School of Electrical Engineering, University of South China, Hengyang, 421001 (China); Rao, Jun; Huang, Mei; Kang, Zihua; Feng, Kun [Southwestern Institute of Physics, Chengdu, 610041 (China); Huang, Jiaqi [School of Electrical Engineering, University of South China, Hengyang, 421001 (China)

    2017-04-15

    Highlights: • Completed design of all solid-state high-voltage power supply for gyrotron body on HL-2M ECRH. • Consist of 58 PSM modules and one BUCK module, controlled by DSP system. • Fabricated full voltage 35 kV, 200 mA BPS and tested in dummy load. • The BPS can operate in three modes: single pulse mode, multi-pulse modulation mode and the six-level preset mode. - Abstract: Gyrotron plays an important role in the research of electron cyclotron resonance heating (ECRH) on Tokomak. The high-frequency switched power supply technology and pulse step modulation (PSM) technology are used in the development of the all solid high-voltage body power supply (BPS) for 1 MW/105 GHz Gyrotron on ECRH system. Firstly, the basic structure of the BPS and its control system are introduced. Secondly, the software control algorithm of voltage stabilization and modulate method are developed. Finally, the design is verified by the experiments. The experimental results of the single pulse mode, the multi-pulse modulation mode and the six-level preset mode, are shown. The output voltage of the power supply can reach 35 kV and the current at about 200 mA, which are adjustable in the full range. The maximum modulation frequency can reach 1 kHz and the front edge of the pulse can be adjust from 0 to 3 ms and the accuracy of the output voltage is less than 100 V. The results show that the control method is feasible and can be applied to other high power microwave sources.

  18. Design of a system for conversion of gyrotron output into a gaussian beam

    Energy Technology Data Exchange (ETDEWEB)

    Ogawa, I. [Faculty of Engineering, Fukui Univ., Fukui (Japan); Idehara, T.; Sabchevski, S.; Glyavin, M.; Mitsudo, S. [Research Center for Development of Far-Infrared Region, Fukui Univ., Fukui (Japan); Sato, M.; Kawahata, K. [National Inst. for Fusion Science, Toki, Gifu (Japan); Brand, G.F. [School of Physics, Univ. of Sydney, NSW (Australia)

    2000-03-01

    A quasi-optical system consisting of a quasi-optical antenna, two ellipsoidal mirrors and a filter to block sidelobes can convert four TE{sub 1n} mode outputs of the Gyrotron FU IVA into gaussian-like beams with waist size of 16.3 mm. This system can convert three TE{sub 0n} mode outputs into bigaussian-like beams with waist size of 23.5 mm in width and 16.4 mm in length. (author)

  19. Test of a two-dimensionally focusing quasi-optical antenna using a gyrotron

    Energy Technology Data Exchange (ETDEWEB)

    Idehara, T.; Tatsukawa, T.; Brand, G.F.; Fekete, P.W.; Moore, K.J.

    1989-05-01

    A quasi-optical antenna having one elliptical reflector and one parabolic reflector has been built for millimeter wave scattering measurements on the TORTUS tokamak plasma at the University of Sydney. This letter reports the first demonstration of the properties of such an antenna using a gyrotron millimeter wave source. Its advantages are (1) good two-dimensional focusing (along the major radius and the toroidal directions) and (2) easy movement of the focus across the diameter of the plasma by changing the orientation of the parabolic reflector.

  20. Particle simulation of a ka-band gyrotron traveling wave amplifier

    Energy Technology Data Exchange (ETDEWEB)

    Xu Shouxi; Liu Pukun; Zhang Shichang; Du Chaohai; Xue Qianzhong; Geng Zhihui; Su Yinong [Key Laboratory of High Power Microwave Sources and Technologies, Institute of Electronics, Chinese Academy of Sciences, P.O. Box 2652, Beijing 100190 (China)

    2011-08-15

    The design of a ka-band gyrotron traveling wave (gyro-TWT) amplifier is presented. The gyro-TWT amplifier with a severed structure operates in the fundamental harmonic TE{sub 01} circular electric mode. The beam-wave interaction is studied by using a particle-in-cell (PIC) code. The simulations predict that the amplifier can produce an output peak power of over 155 kW, 22% efficiency, 23 dB gain, and a 3 dB bandwidth of 2 GHz for a 70 kV, 10 A electron beam with an axial velocity spread {Delta}v{sub z}/v{sub z}=5%.

  1. Coupler for coupling gyrotron whispering gallery mode RF into HE11 waveguide

    Science.gov (United States)

    Neilson, Jeffrey M

    2015-02-24

    A cylindrical waveguide with a mode converter transforms a whispering gallery mode from a gyrotron cylindrical waveguide with a helical cut launch edge to a quasi-Gaussian beam suitable for conveyance through a corrugated waveguide. This quasi-Gaussian beam is radiated away from the waveguide using a spiral cut launch edge, which is in close proximity to a first mode converting reflector. The first mode converting reflector is coupled to a second mode converting reflector which provides an output free-space HE11 mode wave suitable for direct coupling into a corrugated waveguide. The radiated beam produced at the output of the second mode converting reflector is substantially circular.

  2. [Effects of Celosia argentea and Cucurbita moschata extracts on anti-DNP IgE antibody production in mice].

    Science.gov (United States)

    Imaoka, K; Ushijima, H; Inouye, S; Takahashi, T; Kojima, Y

    1994-05-01

    We have already reported that the Perilla frutescens extract (PFE) suppressed anti-DNA IgE antibody production in mice. In this study, we prepared extracts of Celosia argentea L. (CAE) and Cucurbita moschata Duch (CME), which are Chinese herbal medicines like Perilla frutescens, and examined the effects on anti-DNP antibody responses in mice. To examine the effects of CAE & CME on primary antibody responses, CAE & CME were intraperitoneally injected the day before primary immunization of DNP-ovalbumin. Anti-DNP antibody production was markedly suppressed. Then, we examined the effects on secondary antibody responses. CEA & CME were injected only the day before secondary immunization. Anti-DNP IgE production was markedly suppressed, but IgG responses were not affected. It was also found that mitogenic activity occurred in CAE & CME dose dependently in vitro. These effects of CAE & CME were superior to that of PFE. These results suggest that CAE & CME may be more useful than PFE for the suppression of IgE antibody in certain allergic disorders.

  3. Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP-Supported Solid-State NMR Spectroscopy

    NARCIS (Netherlands)

    Jantschke, Anne; Koers, Eline; Mance, Deni; Weingarth, Markus; Brunner, Eike; Baldus, Marc

    2015-01-01

    Diatom biosilica is an inorganic/organic hybrid with interesting properties. The molecular architecture of the organic material at the atomic and nanometer scale has so far remained unknown, in particular for intact biosilica. A DNP-supported ssNMR approach assisted by microscopy, MS, and MD

  4. Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP-Supported Solid-State NMR Spectroscopy

    NARCIS (Netherlands)

    Jantschke, Anne; Koers, Eline; Mance, Deni; Weingarth, Markus; Brunner, Eike; Baldus, Marc

    2015-01-01

    Diatom biosilica is an inorganic/organic hybrid with interesting properties. The molecular architecture of the organic material at the atomic and nanometer scale has so far remained unknown, in particular for intact biosilica. A DNP-supported ssNMR approach assisted by microscopy, MS, and MD simulat

  5. Dynamic Nuclear Polarization (DNP) solid-state NMR spectroscopy, a new approach to study humic material?

    Science.gov (United States)

    Knicker, Heike; Lange, Sascha; van Rossum, Barth; Oschkinat, Hartmut

    2016-04-01

    Compared to solution NMR spectroscopy, solid-state NMR spectra suffer from broad resonance lines and low resolution. This could be overcome by the use of 2-dimenstional solid-state NMR pulse sequences. Until recently, this approach has been unfeasible as a routine tool in soil chemistry, mainly because of the low NMR sensitivity of the respective samples. A possibility to circumvent those sensitivity problems represents high-field Dynamic Nuclear Polarization (DNP) solid-state NMR spectroscopy (Barnes et al., 2008), allowing considerable signal enhancements (Akbey et al., 2010). This is achieved by a microwave-driven transfer of polarization from a paramagnetic center to nuclear spins. Application of DNP to MAS spectra of biological systems (frozen solutions) showed enhancements of the factor 40 to 50 (Hall et al., 1997). Enhancements of this magnitude, thus may enable the use of at least some of the 2D solid-state NMR techniques that are presently already applied for pure proteins but are difficult to apply to soil peptides in their complex matrix. After adjusting the required acquisition parameters to the system "soil organic matter", lower but still promising enhancement factors were achieved. Additional optimization was performed and allowed the acquisition of 2D 13C and 15N solid-state NMR spectra of humified 13C and 15N enriched plant residues. Within the present contribution, the first solid-state DNP NMR spectra of humic material are presented. Those data demonstrate the great potential of this approach which certainly opens new doors for a better understanding of biochemical processes in soils, sediments and water. Akbey, Ü., Franks, W.T., Linden, A., Lange, S., Griffin, R.G., van Rossum, B.-J., Oschkinat, H., 2010. Dynamic nuclear polarization of deuterated proteins. Angewandte Chemie International Edition 49, 7803-7806. Barnes, A.B., De Paëpe, G., van der Wel, P.C.A., Hu, K.N., Joo, C.G., Bajaj, V.S., Mak-Jurkauskas, M.L., Sirigiri, J.R., Herzfeld, J

  6. AN ANP/DNP ANALYSIS OF ECONOMIC ELEMENTS IN TODAY'S WORLD NETWORK ECONOMY

    Institute of Scientific and Technical Information of China (English)

    Petr FIALA

    2006-01-01

    The network economy is a term for today's global relationship among economic elements characterized by massive connectivity. The central act of the new era is to connect everything to everything in deep web networks at many levels of mutually interdependent relations, where resources and activities are shared, markets are enlarged and costs and risk are reduced. Network systems contain both positive and negative feedback. A variety of feedback processes create complex system behavior.For such a network the Analytic Network Process (ANP) approach seems to be very appropriate. The ANP method makes it possible to deal systematically with all kinds of dependence and feedback in the system. Dynamic models try to reflect changes in real or simulated time and take into account that the network model components are constantly evolving. Dynamic models use concepts of state variables,flows, and feedback processes. The Dynamic Network Process (DNP) is anextension of ANP that can deal with time dependent priorities in a networked economy.

  7. Development of problem-oriented software packages for numerical studies and computer-aided design (CAD) of gyrotrons

    Science.gov (United States)

    Damyanova, M.; Sabchevski, S.; Zhelyazkov, I.; Vasileva, E.; Balabanova, E.; Dankov, P.; Malinov, P.

    2016-03-01

    Gyrotrons are the most powerful sources of coherent CW (continuous wave) radiation in the frequency range situated between the long-wavelength edge of the infrared light (far-infrared region) and the microwaves, i.e., in the region of the electromagnetic spectrum which is usually called the THz-gap (or T-gap), since the output power of other devices (e.g., solid-state oscillators) operating in this interval is by several orders of magnitude lower. In the recent years, the unique capabilities of the sub-THz and THz gyrotrons have opened the road to many novel and future prospective applications in various physical studies and advanced high-power terahertz technologies. In this paper, we present the current status and functionality of the problem-oriented software packages (most notably GYROSIM and GYREOSS) used for numerical studies, computer-aided design (CAD) and optimization of gyrotrons for diverse applications. They consist of a hierarchy of codes specialized to modelling and simulation of different subsystems of the gyrotrons (EOS, resonant cavity, etc.) and are based on adequate physical models, efficient numerical methods and algorithms.

  8. Operation experiences of the super conducting magnet for a gyrotron of the JT-60U ECH system

    Energy Technology Data Exchange (ETDEWEB)

    Igarashi, Koichi; Seki, Masami; Shimono, Mitsugu; Terakado, Masayuki; Ishii, Kazuhiro; Takahashi, Masami [Japan Atomic Energy Research Inst., Naka, Ibaraki (Japan). Naka Fusion Research Establishment

    2003-03-01

    The JT-60U electron cyclotron heating (ECH) system can heat plasmas locally and drive a plasma current with four 1 MW-5 sec gyrotrons. The super conducting magnets (SCM) are required for oscillation of the gyrotron at a working frequency of 110 GHz. The SCM provides a high magnetic field of 4.5T at the cavity inside the gyrotron. This SCM system is characterized by 1) operation without liquid Helium owing to a 4K-refrigerator applied to the magnetic coils, 2) easy maintenance. Operational experiences about the SCM system through a long term experiment for a high power gyrotron are very valuable. According to those operational experiences, it is clarified the 4K-refrigerator should be renewed in order to keep low temperature of the SCM. It is also found that 200 hours or less are required for the super conducting condition (<5K) after long stopping time of the refrigerator up to 150 hours. This is useful information for making a plan about ECH experiments. (author)

  9. Design of the Collective Thomson scattering (CTS) system by using 170-GHz gyrotron in the KSTAR

    Science.gov (United States)

    Park, Min; Kim, Sun-Ho; Kim, Sung-Kyu; Lee, Kyu-Dong; Wang, Son-Jong

    2014-10-01

    The physics of energetic ions is one of the primary subjects to be understood toward the realization of a nuclear fusion power plant. Collective Thomson scattering (CTS) offers the possibility to diagnose the fast ions and the alpha particles in burning plasmas. Spatially- and temporally-resolved one-dimensional velocity distributions of the fast ions can be obtained from the scattered radiation with fewer geometric constraints by utilizing millimeter waves from a high-power gyrotron as a probe beam. We studied the feasibility of CTS fast-ion measurements in the KSTAR by calculating the spectral density functions. Based on that, we suggest a design for the CTS system that uses the currently-operating 170-GHz gyrotron for electron cyclotron heating (ECH) and electron cyclotron current drive (ECCD) in the KSTAR. The CTS system is presented as two subsystems: the antenna system and the heterodyne receiver system. The design procedure for an off-axis ellipsoidal mirror is described, and the CTS system requirements are discussed.

  10. High current multicharged metal ion source using high power gyrotron heating of vacuum arc plasma.

    Science.gov (United States)

    Vodopyanov, A V; Golubev, S V; Khizhnyak, V I; Mansfeld, D A; Nikolaev, A G; Oks, E M; Savkin, K P; Vizir, A V; Yushkov, G Yu

    2008-02-01

    A high current, multi charged, metal ion source using electron heating of vacuum arc plasma by high power gyrotron radiation has been developed. The plasma is confined in a simple mirror trap with peak magnetic field in the plug up to 2.5 T, mirror ratio of 3-5, and length variable from 15 to 20 cm. Plasma formed by a cathodic vacuum arc is injected into the trap either (i) axially using a compact vacuum arc plasma gun located on axis outside the mirror trap region or (ii) radially using four plasma guns surrounding the trap at midplane. Microwave heating of the mirror-confined, vacuum arc plasma is accomplished by gyrotron microwave radiation of frequency 75 GHz, power up to 200 kW, and pulse duration up to 150 micros, leading to additional stripping of metal ions by electron impact. Pulsed beams of platinum ions with charge state up to 10+, a mean charge state over 6+, and total (all charge states) beam current of a few hundred milliamperes have been formed.

  11. Beam-wave interaction behavior of a 35 GHz metal PBG cavity gyrotron

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Ashutosh, E-mail: asingh.rs.ece@iitbhu.ac.in [Faculty of Physical Sciences, Institute of Natural Sciences and Humanities Shri Ramswaroop Memorial University, Lucknow-Deva Road, Uttar Pradesh-225003 (India); Center of Research in Microwave Tubes, Department of Electronics Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi-221005 (India); Jain, P. K. [Center of Research in Microwave Tubes, Department of Electronics Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi-221005 (India)

    2014-09-15

    The RF behavior of a 35 GHz photonic band gap (PBG) cavity gyrotron operating in TE{sub 041}-like mode has been presented to demonstrate its single mode operation capability. In this PBG cavity gyrotron, the conventional tapered cylindrical cavity is replaced by a metal PBG cavity as its RF interaction structure. The beam-wave interaction behavior has been explored using time dependent multimode nonlinear analysis as well as through 3D PIC simulation. Metal PBG cavity is treated here similar to that of a conventional cylindrical cavity for the desired mode confinement. The applied DC magnetic field profile has been considered uniform along the PBG cavity length both in analysis as well as in simulation. Electrons energy and phase along the interaction length of the PBG cavity facilitates bunching mechanism as well as energy transfer phenomena from the electron beam to the RF field. The RF output power for the TE{sub 041}-like design mode as well as nearby competing modes have been estimated and found above to 100 kW in TE{sub 041}-like mode with ∼15% efficiency. Results obtained from the analysis and the PIC simulation are found in agreement within 8% variation, and also it supports the single mode operation, as the PBG cavity does not switch into other parasitic modes in considerably large range of varying DC magnetic field, contrary to the conventional cylindrical cavity interaction structure.

  12. Conceptual designs of E × B multistage depressed collectors for gyrotrons

    Science.gov (United States)

    Wu, Chuanren; Pagonakis, Ioannis Gr.; Gantenbein, Gerd; Illy, Stefan; Thumm, Manfred; Jelonnek, John

    2017-04-01

    Multistage depressed collectors are challenges for high-power, high-frequency fusion gyrotrons. Two concepts exist in the literature: (1) unwinding the spent electron beam cyclotron motion utilizing non-adiabatic transitions of magnetic fields and (2) sorting and collecting the electrons using the E × B drift. To facilitate the collection by the drift, the hollow electron beam can be transformed to one or more thin beams before applying the sorting. There are many approaches, which can transform the hollow electron beam to thin beams; among them, two approaches similar to the tilted electric field collectors of traveling wave tubes are conceptually studied in this paper: the first one transforms the hollow circular electron beam to an elongated elliptic beam, and then the thin elliptic beam is collected by the E × B drift; the second one splits an elliptic or a circular electron beam into two arc-shaped sheet beams; these two parts are collected individually. The functionality of these concepts is proven by CST simulations. A model of a three-stage collector for a 170 GHz, 1 MW gyrotron using the latter approach shows 76% collector efficiency while taking secondary electrons and realistic electron beam characteristics into account.

  13. Effect of ion compensation of the beam space charge on gyrotron operation

    Energy Technology Data Exchange (ETDEWEB)

    Fokin, A. P.; Glyavin, M. Yu. [Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod 603950 (Russian Federation); Nusinovich, G. S. [Institute for Research in Electronics and Applied Physics, University of Maryland, College Park, Maryland 20742-3511 (United States)

    2015-04-15

    In gyrotrons, the coherent radiation of electromagnetic waves takes place when the cyclotron resonance condition between the wave frequency and the electron cyclotron frequency or its harmonic holds. The voltage depression caused by the beam space charge field changes the relativistic cyclotron frequency and, hence, can play an important role in the beam-wave interaction process. In long pulse and continuous-wave regimes, the beam space charge field can be partially compensated by the ions, which appear due to the beam impact ionization of neutral molecules of residual gases in the interaction space. In the present paper, the role of this ion compensation of the beam space charge on the interaction efficiency is analyzed. We also analyze the effect of the electron velocity spread on the limiting currents and discuss some effects restricting the ion-to-beam electron density ratio in the saturation stage. It is shown that the effect of the ion compensation on the voltage depression caused by the beam space charge field can cause significant changes in the efficiency of gyrotron operation and, in some cases, even result in the break of oscillations.

  14. The 5.8 T Cryogen-Free Gyrotron Superconducting Magnet System on HL-2A

    Science.gov (United States)

    Xia, Donghui; Huang, Mei; Zhou, Jun; Bai, Xingyu; Zheng, Tieliu; Rao, Jun; Zhuang, Ge

    2014-04-01

    A 5.8 T cryogen-free superconducting magnet (SCM) system with a warm bore hole of 160 mm in diameter, used for gyrotrons operating in the frequency range from 68 GHz to 140 GHz, is installed on the site of the HL-2A tokamak. The SCM consists of two separate solenoidal magnetic coils connected in series, a 4.2 K Gifford-McMahon (GM) refrigerator, a compressor, a coil power supply and two temperature monitors. The performance, test and preliminary experimental results of this SCM system are described in this paper. The magnetic field distribution was measured along the axis, and a dummy tube was used for adjusting the magnet system. Finally, the magnet was used for the operation of a 68 GHz/500 kW gyrotron, which is part of an electron cyclotron resonance heating (ECRH) system. With an additional auxiliary coil and after adjusting the magnet system, a maximum output power for the ECRH system of up to 400 kW was achieved.

  15. Preparation and LSC Standardization of ''89Sr (DNP) Using the CIEMAT/NIST Method; Preparacion del ''89Sr(DNP) y calibracion por centelleo liquido, mediante el metodo CIEMAT/NIST

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez Barquero, L.; Los Arcos Merino, J. M.; Grau Malonda, A.

    1994-07-01

    A procedure for preparation of liquid scintillation counting samples of the strontium DNP complex, labelled with ''89Sr, is described, the chemical quench, the counting stability and spectral evolution of this compound is studied in six scintillators, Toluene, Toluene-alcohol, Dioxane-naphthalene, HiSafe II, Ultima- Gold and Instagel. The liquid scintillation standardization of 89Sr-DNP by the CIEMAT/NIST method, using HiSafe II and Ultima-Gold scintillators, has been carried out. The discrepancies between experimental and computed efficiencies are lower than 0.38% and 0.48%, respectively. The solution has been standardized in terms of activity concentration to an overall uncertainty of 0,38%. (Author) 10 refs.

  16. Implementation of DNP3.0 Protocol in Integrated Automation System Based on RTU%DNP3.0在基于RTU的综合自动化系统中的实现

    Institute of Scientific and Technical Information of China (English)

    安艳萍; 申群太

    2006-01-01

    DNP是目前电力系统自动化产品市场上的一种主流通信规约,简要介绍了DNP3.0的基本结构和功能,结合基于RTU的电力综合自动化系统开发实例,给出了DNP协议部分功能的具体实现方法,通过该协议系统实现了"四遥"功能.现场运行结果显示,DNP3.0协议具有较高的通信效率和通信可靠性,运行方式灵活多样,误码率极低,完全能够满足电力系统实时性和可靠性的要求.

  17. A comparison investigation of DNP-binding effects to HSA and HTF by spectroscopic and molecular modeling techniques.

    Science.gov (United States)

    Zolfagharzadeh, Mahboobeh; Pirouzi, Maliheh; Asoodeh, Ahmad; Saberi, Mohammad Reza; Chamani, Jamshidkhan

    2014-12-01

    This paper describes the interaction between 2,4-dinitrophenol (DNP) with the two drug carrier proteins - human serum albumin (HSA) and human holo transferrin (HTF). Hence, binding characteristics of DNP to HSA and HTF were analyzed by spectroscopic and molecular modeling techniques. Based on results obtained from fluorescence spectroscopy, DNP had a strong ability to quench the intrinsic fluorescence of HSA and HTF through a static quenching procedure. The binding constant and the number of binding sites were calculated as 2.3 × 10(11) M(-1) and .98 for HSA, and 1.7 × 10(11) M(-1) and 1.06 for HTF, respectively. In addition, synchronous fluorescence results showed that the microenvironment of Trp had a slight tendency of increasing its hydrophobicity, whereas the microenvironment of the Tyr residues of HSA did not change and that of HTF showed a significant trend (red shift of about 4 nm) of an increase in polarity. The distance between donor and acceptor was obtained by the Förster energy according to fluorescence resonance energy transfer, and was found to be 3.99 and 3.72 nm for HSA and HTF, respectively. The critical induced aggregation concentration (CCIAC) of the drug on both proteins was determined and confirmed by an inflection point of the zeta potential behavior. Circular dichroism data revealed that the presence of DNP caused a decrease of the α-helical content of HSA and HTF, and induced a remarkable mild denaturation of both proteins. The molecular modeling data confirmed our experimental results. This study is deemed useful for determining drug dosage.

  18. Formation of Multicharged Metal Ions in Vacuum Arc Plasma Heated by Gyrotron Radiation%Formation of Multicharged Metal Ions in Vacuum Arc Plasma Heated by Gyrotron Radiation

    Institute of Scientific and Technical Information of China (English)

    G. Yu. YUSHKOV; K. P. SAVKIN; A. G. NIKOLAEV; E. M. OKS; A.V. VODOPYANOV; I. V. IZOTOV; D. A. MANSFELD

    2011-01-01

    A new method for the generation of high charged state metal ion beams is developed. This method is based on microwave heating of vacuum arc plasma in a magnetic trap under electron cyclotron resonance (ECR) conditions. Two gyrotrons for plasma heating were used, which were with the following parameters. The first is with a wave frequency of 37.5 GHz, a pulse duration of 1 ms and power of 100 kW, another is with 75 GHz, 0.15 ms and 400 kW. Two different magnetic traps were considered for vacuum arc plasma confinement. The first one is a simple mirror trap. Such system was already investigated and could provide high charge state ions. The second trap was with a cusp magnetic field configuration with native "minimum-B" field structure. Two different ways of metal plasma injection into the magnetic trap were used. The first one is an axial injection from an arc source located out of the trap, and the second is a radial injection from four arc sources mounted at the center of the trap. Both traps provide up to 200 eMA of ion beam current for platinum ions with highest charge state 10+. Ion beams were successfully extracted from the plasma and accelerated by a voltage of up to 20 kV.

  19. DNP-enhanced ultrawideline (207)Pb solid-state NMR spectroscopy: an application to cultural heritage science.

    Science.gov (United States)

    Kobayashi, Takeshi; Perras, Frédéric A; Murphy, Anna; Yao, Yao; Catalano, Jaclyn; Centeno, Silvia A; Dybowski, Cecil; Zumbulyadis, Nicholas; Pruski, Marek

    2017-03-14

    Dynamic nuclear polarization (DNP) is used to enhance the (ultra)wideline (207)Pb solid-state NMR spectra of lead compounds of relevance in the preservation of cultural heritage objects. The DNP SSNMR experiments enabled, for the first time, the detection of the basic lead carbonate phase of the lead white pigment by (207)Pb SSNMR spectroscopy. Variable-temperature experiments revealed that the short T'2 relaxation time of the basic lead carbonate phase hinders the acquisition of the NMR signal at room temperature. We additionally observe that the DNP enhancement is twice as large for lead palmitate (a lead soap, which is a degradation product implicated in the visible deterioration of lead-based oil paintings), than it is for the basic lead carbonate. This enhancement has allowed us to detect the formation of a lead soap in an aged paint film by (207)Pb SSNMR spectroscopy; which may aid in the detection of deterioration products in smaller samples removed from works of art.

  20. Crystal Structure and Thermal Decomposition Mechanism of [K(DNP)(H2O)0.5]n

    Institute of Scientific and Technical Information of China (English)

    MA, Gui-Xia(马桂霞); ZHANG, Tong-Lai(张同来); ZHANG, Jian-Guo(张建国); SHAO, Bing(邵兵); LI, Yu-Feng(李玉锋); SONG, Jiang-Chuang(宋江闯); YU, Kai-Bei(郁开北)

    2004-01-01

    The new coordination polymer, [K(DNP)(H2O)0.5]n (DNP=2,4-dinitrophenol) was prepared by the reaction of 2,4-dinitrophenol with potassium hydroxide. The molecular structure was determined by X-ray single-crystal diffraction analysis, FT-IR techniques, and elemental analysis. The crystal is monoclinic, space group C(2)/c with the empirical formula of C6H4N2O5.5K, Mr=231.21. The unit cell parameters are as follows: a=2.0789(3) nm, b=1.2311(2) nm, c=0.7203(1) nm, β=109.611(15)°, V=1.7366(4) nm3, Z=4, Dc=1.769 g/cm3, F(000)=936, μ (Mo Kα)=0.617 mm-1. The potassium ions coordinate with all the oxygen atoms of phenolic hydroxyl group and nitro-group of different 2,4-dinitrophenol (DNP) ligands with high coordination number, and form a good cross-linked three-dimensional net structure of which makes the complex with good stabilities. The thermal decomposition of [K(DNP)(H2O)0.5]n has only an intense exothermic process in the temperature range of 338-343 ℃ corresponding to a mass loss of 75.02%. The final decomposition residue is potassium isocyanide.

  1. Velocity-space tomography of fusion plasmas by collective Thomson scattering of gyrotron radiation

    DEFF Research Database (Denmark)

    Salewski, Mirko; Jacobsen, A.S.; Jensen, Thomas;

    2016-01-01

    We propose a diagnostic capable of measuring 2D fast-ion velocity distribution functions 푓2퐷푣 in the MeV-range in magnetized fusion plasmas. Today velocity-space tomography based on fast-ion D훼 spectroscopy is regularly used to measure 푓2퐷푣 for ion energies below 100 keV. Unfortunately, the signal......-tonoise ratio becomes fairly low for MeV-range ions. Ions at any energy can be detected well by collective Thomson scattering of mm-wave radiation from a high-power gyrotron. We demonstrate how collective Thomson scattering can be used to measure 푓2퐷푣 in the MeV-range in reactor relevant plasmas...

  2. Systematic Observation of Time-Dependent Phenomena in the RF Output Spectrum of High Power Gyrotrons

    Directory of Open Access Journals (Sweden)

    Kern Stefan

    2012-09-01

    Full Text Available At IHM/KIT, high power gyrotrons with conventional cavity (e.g. 1 MW CW at 140 GHz for the stellarator Wendelstein 7-X and coaxial cavity (2 MW shortpulse at 170 GHz for ITER for fusion applications are being developed and verified experimentally. Especially with respect to the problem of parasitic RF oscillations in the beam tunnel of some W7-X tubes, investigations of the gyrotron RF output spectrum have proved to be a valuable source of diagnostic information. Signs of transient effects in millisecond pulses, like frequency switching or intermittent low-frequency modulation, have indicated that truly time-dependent measurements with high frequency resolution and dynamic range could give deeper insight into these phenomena. In this paper, an improved measurement system is presented, which employs a fast oscilloscope as receiver. Shorttime Fourier transform (STFT is applied to the time-domain signal, yielding time-variant spectra with frequency resolutions only limited by acquisition length and STFT segmentation choice. Typical reasonable resolutions are in the range of 100 kHz to 10 MHz with a currently memory-limited maximum acquisition length of 4 ms. A key feature of the system consists in the unambiguity of frequency measurement: The system receives through two parallel channels, each using a harmonic mixer (h = 9 – 12 to convert the signal from RF millimeter wave frequencies (full D-Band, 110 – 170 GHz to IF (0 – 3 GHz. For each IF output signal of each individual mixer, injection side and receiving harmonic are initially not known. Using accordingly determined LO frequencies, this information is retrieved from the redundancy of the channels, yielding unambiguously reconstructed RF spectra with a total span of twice the usable receiver IF bandwidth, up to ≈ 6 GHz in our case. Using the system, which is still being improved continuously, various transient effects like cavity mode switching, parasitic oscillation frequency variation

  3. Continuous-flow DNP polarizer for MRI applications at 1.5 T

    Science.gov (United States)

    Denysenkov, V.; Terekhov, M.; Maeder, R.; Fischer, S.; Zangos, S.; Vogl, T.; Prisner, T. F.

    2017-03-01

    Here we describe a new hyperpolarization approach for magnetic resonance imaging applications at 1.5 T. Proton signal enhancements of more than 20 were achieved with a newly designed multimode microwave resonator situated inside the bore of the imager and used for Overhauser dynamic nuclear polarization of the water proton signal. Different from other approaches in our setup the hyperpolarization is achieved continuously by liquid water flowing through the polarizer under continuous microwave excitation. With an available flow rate of up to 1.5 ml/min, which should be high enough for DNP MR angiography applications in small animals like mice and rats. The hyperpolarized liquid cooled to physiological temperature can be routed by a mechanical switch to a quartz capillary for injection into the blood vessels of the target object. This new approach allows hyperpolarization of protons without the need of an additional magnet and avoids the losses arising from the transfer of the hyperpolarized solution between magnets. The signal-to-noise improvement of this method is demonstrated on two- and three-dimensional phantoms of blood vessels.

  4. Hyperpolarized [2-13C]-fructose: a hemiketal DNP substrate for in vivo metabolic imaging.

    Science.gov (United States)

    Keshari, Kayvan R; Wilson, David M; Chen, Albert P; Bok, Robert; Larson, Peder E Z; Hu, Simon; Van Criekinge, Mark; Macdonald, Jeffrey M; Vigneron, Daniel B; Kurhanewicz, John

    2009-12-09

    Hyperpolarized (13)C labeled molecular probes have been used to investigate metabolic pathways of interest as well as facilitate in vivo spectroscopic imaging by taking advantage of the dramatic signal enhancement provided by DNP. Due to the limited lifetime of the hyperpolarized nucleus, with signal decay dependent on T(1) relaxation, carboxylate carbons have been the primary targets for development of hyperpolarized metabolic probes. The use of these carbon nuclei makes it difficult to investigate upstream glycolytic processes, which have been related to both cancer metabolism as well as other metabolic abnormalities, such as fatty liver disease and diabetes. Glucose carbons have very short T(1)s (metabolic probe of glycolysis. However, the pentose analogue fructose can also enter glycolysis through its phosphorylation by hexokinase and yield complementary information. The C(2) of fructose is a hemiketal that has a relatively longer relaxation time (approximately 16 s at 37 degrees C) and high solution state polarization (approximately 12%). Hyperpolarized [2-(13)C]-fructose was also injected into a transgenic model of prostate cancer (TRAMP) and demonstrated difference in uptake and metabolism in regions of tumor relative to surrounding tissue. Thus, this study demonstrates the first hyperpolarization of a carbohydrate carbon with a sufficient T(1) and solution state polarization for ex vivo spectroscopy and in vivo spectroscopic imaging studies.

  5. Systematic cavity design approach for a multi-frequency gyrotron for DEMO and study of its RF behavior

    Science.gov (United States)

    Kalaria, P. C.; Avramidis, K. A.; Franck, J.; Gantenbein, G.; Illy, S.; Pagonakis, I. Gr.; Thumm, M.; Jelonnek, J.

    2016-09-01

    High frequency (>230 GHz) megawatt-class gyrotrons are planned as RF sources for electron cyclotron resonance heating and current drive in DEMOnstration fusion power plants (DEMOs). In this paper, for the first time, a feasibility study of a 236 GHz DEMO gyrotron is presented by considering all relevant design goals and the possible technical limitations. A mode-selection procedure is proposed in order to satisfy the multi-frequency and frequency-step tunability requirements. An effective systematic design approach for the optimal design of a gradually tapered cavity is presented. The RF-behavior of the proposed cavity is verified rigorously, supporting 920 kW of stable output power with an interaction efficiency of 36% including the considerations of realistic beam parameters.

  6. Thermal and Structural Analysis and its Effect on Beam-Wave Interaction for 170-GHz, 1-MW Gyrotron Cavity

    Science.gov (United States)

    Kumar, Anil; Kumar, Nitin; Singh, Udaybir; Khatun, Hasina; Vyas, V.; Sinha, A. K.

    2012-04-01

    In this paper thermal and structural analysis for 170 GHz, 1 MW gyrotron interaction cavity and the effect of structural deformation on beam wave interaction is presented. Finite element analysis codes ANSYS has been used for the thermal and structural analysis. Electromagnetic simulator-MAGIC, a Particle-in-Cell (PIC) code, has been used to carry out the effect of the radial expansion of the interaction cavity on beam wave interaction. The change in output power and resonant frequency for operating mode TE34,10 due to thermal expansion is 10 kW and 0.07 GHz, respectively. These values are under the tolerance limit of power and frequency of the gyrotron. The major variation is found in the power growth stability time.

  7. Free-electron masers vs. gyrotrons prospects for high-power sources at millimeter and submillimeter wavelengths

    CERN Document Server

    Thumm, M K

    2002-01-01

    The possible applications of high-power millimeter (mm) and sub-mm waves from free-electron masers (FEMs) and gyro-devices span a wide range of technologies. The plasma physics community has already taken advantage of recent advances in applying high-power mm waves generated by long pulse or continuous wave (CW) gyrotron oscillators and short pulse very high-power FEMs in the areas of RF-plasma production, heating, non-inductive current drive, plasma stabilization and active plasma diagnostics for magnetic confinement thermonuclear fusion research, such as electron cyclotron resonance heating (28-170 GHz), electron cyclotron current drive , collective Thomson scattering , microwave transmission and heat-wave propagation experiments. Continuously frequency tunable FEMs could widen these fields of applications. Another important application of CW gyrotrons is industrial materials processing, e.g. sintering of high-performance functional and structural nanostructured ceramics. Sub-mm wave sources are employed in...

  8. UTAUT2 Based Predictions of Factors Influencing the Technology Acceptance of Phablets by DNP

    Directory of Open Access Journals (Sweden)

    Chi-Yo Huang

    2015-01-01

    Full Text Available The smart mobile devices have emerged during the past decade and have become one of the most dominant consumer electronic products. Therefore, exploring and understanding the factors which can influence the acceptance of novel mobile technology have become the essential task for the vendors and distributors of mobile devices. The Phablets, integrated smart devices combining the functionality and characteristics of both tablet PCs and smart phones, have gradually become possible alternatives for smart phones. Therefore, predicting factors which can influence the acceptance of Phablets have become indispensable for designing, manufacturing, and marketing of such mobile devices. However, such predictions are not easy. Meanwhile, very few researches tried to study related issues. Consequently, the authors aim to explore and predict the intentions to use and use behaviors of Phablets. The second generation of the Unified Theory of Acceptance and Use of Technology (UTAUT2 is introduced as a theoretic basis. The Decision Making Trial and Evaluation Laboratory (DEMATEL based Network Process (DNP will be used to construct the analytic framework. In light of the analytic results, the causal relationships being derived by the DEMATEL demonstrate the direct influence of the habit on other dimensions. Also, based on the influence weights being derived, the use intention, hedonic motivation, and performance expectancy are the most important dimensions. The analytic results can serve as a basis for concept developments, marketing strategy definitions, and new product designs of the future Phablets. The proposed analytic framework can also be used for predicting and analyzing consumers’ preferences toward future mobile devices.

  9. Towards the optimization of the thermal–hydraulic performance of gyrotron collectors

    Energy Technology Data Exchange (ETDEWEB)

    Savoldi, Laura; Bertani, Cristina [Dipartimento Energia, Politecnico di Torino, 10129 Torino (Italy); Cau, Francesca; Cismondi, Fabio [F4E, Barcelona (Spain); Gantenbein, Gerd; Illy, Stefan [Karlsruhe Institute of Technology (KIT), Institute for Pulsed Power and Microwave Technology (IHM), Kaiserstr. 12, 76131 Karlsruhe (Germany); Monni, Grazia [Dipartimento Energia, Politecnico di Torino, 10129 Torino (Italy); Rozier, Yoann [Thales Electron Devices, 78141 Vélizy-Villacoublay (France); Zanino, Roberto, E-mail: roberto.zanino@polito.it [Dipartimento Energia, Politecnico di Torino, 10129 Torino (Italy)

    2015-11-15

    Different configurations of water-cooled Cu collector for gyrotrons are investigated using the StarCCM + CFD code, aimed at optimizing its thermal–hydraulic (TH) performance. Although the current collectors show a good performance, the collector can be subjected to transient heat loads, due to the spent electron beam, of up to several tens of MW/m{sup 2}, and there is an interest to increase the gyrotron output power in the future. Furthermore, an optimized cooling will lead to improved reliability and lifetime of the collector. Starting from a hypervapotron (HV)-like collector, characterized by 100+ deep rectangular cavities with aspect ratio (AR) = 3, we present in the first part of the paper a single-cavity steady-state parametric analysis of the effect of AR on the heat exhaust capabilities. The investigation is then extended to other collector designs, including circumferential ribs and dimples, in order to assess the options for further improvements of the TH performance. The peak Cu temperature is computed by the code and its minimization is the target of the present optimization exercise. A self-consistent estimate of the heat transfer coefficient between collector and coolant is also obtained, which could be useful for fatigue and lifetime assessments. In the second part of the paper the most promising collector geometries identified in the first part are analyzed in the case of a transient heat load (vertical sweeping), first at the level of a single spatial period of the collector structure, then at the full-collector level. The results of the TH transient analysis are compared with both the results of the first part and with the transient purely thermal analysis of the full collector, showing for all geometries considered in this study a room for cooling efficiency improvement with respect to the HV-like design with AR = 3, at least in the operating conditions considered for this study (V ∼ 4 m/s, almost 100 °C sub-cooling).

  10. Numerical investigation of collector cooling for a 1 MW ITER gyrotron operated with vertical sweeping

    Energy Technology Data Exchange (ETDEWEB)

    Savoldi, Laura; Bertani, Cristina [Dipartimento Energia, Politecnico di Torino (Italy); Cau, Francesca; Cismondi, Fabio [Fusion for Energy, Barcelona (Spain); Gantenbein, Gerd; Illy, Stefan [KIT, Karlsruhe (Germany); Monni, Grazia [Dipartimento Energia, Politecnico di Torino (Italy); Zanino, Roberto, E-mail: roberto.zanino@polito.it [Dipartimento Energia, Politecnico di Torino (Italy)

    2015-11-15

    The present gyrotron designs for EC plasma heating in nuclear fusion reactors require the safe exhaust of a power comparable to that injected into the plasma, in order to keep the maximum temperature below the acceptable value of 300 °C. In this paper, the commercial computational fluid dynamics (CFD) software STAR-CCM+{sup ®} is used to analyze the thermal performance of the annular copper collector of a 1 MW ITER gyrotron, equipped with a hypervapotron structure made of annular fins with rectangular cavities of aspect ratio (depth/width) = 3, cooled by highly subcooled (90–100 °C) pressurized water flowing at ∼4 m/s. It is assumed that the simple vertical sweeping strategy is used to reduce the very high peak heat flux on the collector (up to 30 MW/m{sup 2} transient, 5 MW/m{sup 2} time average), due to the spent electron beam. The 2D steady-state conjugate heat transfer problem is solved assuming azimuthal symmetry and accounting for 2-phase flow. The single-cavity flow and heat transfer problem is considered first, to optimize the mesh and the selection of the turbulence model. For the operating conditions considered in this paper, the full collector (100+ cavities) solution shows that boiling occurs only in a limited number of cavities close to the peaks of the heat flux, with the vapor remaining trapped in the bottom of the cavities, i.e. no full hypervapotron regime should be achieved in these operating conditions. The steady-state analysis allows the numerical evaluation of the heat transfer coefficients between Cu and water; these are then used as input for the simplified, purely thermal (solid only) analysis of the actual transient problem for the full collector. The results of the simplified model, which allows a huge reduction of the computational effort, are successfully benchmarked against those of a comprehensive thermal–hydraulic simulation. The computed peak Cu temperature is below the acceptable limit under the steady-state (time averaged

  11. Mechanisms of amplification of ultrashort electromagnetic pulses in gyrotron traveling wave tube with helically corrugated waveguide

    Science.gov (United States)

    Ginzburg, N. S.; Zotova, I. V.; Sergeev, A. S.; Zaslavsky, V. Yu.; Zheleznov, I. V.; Samsonov, S. V.; Mishakin, S. V.

    2015-11-01

    A time-domain self consistent theory of a gyrotron traveling wave tube with a helically corrugated operating waveguide has been developed. Based on this model, the process of short pulse amplification was studied in regimes of grazing and intersection of the dispersion curves of the electromagnetic wave and the electron beam. In the first case, the possibility of amplification without pulse form distortion was demonstrated for the pulse spectrum width of the order of the gain bandwidth. In the second case, when the electrons' axial velocity was smaller than the wave's group velocity, it was shown that the slippage of the incident signal with respect to the electron beam provides feeding of the signal by "fresh" electrons without initial modulation. As a result, the amplitude of the output pulse can exceed the amplitude of its saturated value for the case of the grazing regime, and, for optimal parameters, the peak output power can be even larger than the kinetic power of the electron beam.

  12. Intense high-frequency gyrotron-based microwave beams for material processing

    Energy Technology Data Exchange (ETDEWEB)

    Hardek, T.W.; Cooke, W.D.; Katz, J.D.; Perry, W.L.; Rees, D.E.

    1997-03-01

    Microwave processing of materials has traditionally utilized frequencies in the 0.915 and 2.45 GHz regions. Microwave power sources are readily available at these frequencies but the relatively long wavelengths can present challenges in uniformly heating materials. An additional difficulty is the poor coupling of ceramic based materials to the microwave energy. Los Alamos National Laboratory scientists, working in conjunction with the National Center for Manufacturing Sciences (NCMS), have assembled a high-frequency demonstration processing facility utilizing gyrotron based RF sources. The facility is primarily intended to demonstrate the unique features available at frequencies as high as 84 GHz. The authors can readily provide quasi-optical, 37 GHz beams at continuous wave (CW) power levels in the 10 kW range. They have also provided beams at 84 GHz at 10 kW CW power levels. They are presently preparing a facility to demonstrate the sintering of ceramics at 30 GHz. This paper presents an overview of the present demonstration processing facility and describes some of the features they have available now and will have available in the near future.

  13. Gyrotron-driven high current ECR ion source for boron-neutron capture therapy neutron generator

    Science.gov (United States)

    Skalyga, V.; Izotov, I.; Golubev, S.; Razin, S.; Sidorov, A.; Maslennikova, A.; Volovecky, A.; Kalvas, T.; Koivisto, H.; Tarvainen, O.

    2014-12-01

    Boron-neutron capture therapy (BNCT) is a perspective treatment method for radiation resistant tumors. Unfortunately its development is strongly held back by a several physical and medical problems. Neutron sources for BNCT currently are limited to nuclear reactors and accelerators. For wide spread of BNCT investigations more compact and cheap neutron source would be much more preferable. In present paper an approach for compact D-D neutron generator creation based on a high current ECR ion source is suggested. Results on dense proton beams production are presented. A possibility of ion beams formation with current density up to 600 mA/cm2 is demonstrated. Estimations based on obtained experimental results show that neutron target bombarded by such deuteron beams would theoretically yield a neutron flux density up to 6·1010 cm-2/s. Thus, neutron generator based on a high-current deuteron ECR source with a powerful plasma heating by gyrotron radiation could fulfill the BNCT requirements significantly lower price, smaller size and ease of operation in comparison with existing reactors and accelerators.

  14. Mechanisms of amplification of ultrashort electromagnetic pulses in gyrotron traveling wave tube with helically corrugated waveguide

    Energy Technology Data Exchange (ETDEWEB)

    Ginzburg, N. S., E-mail: ginzburg@appl.sci-nnov.ru; Zaslavsky, V. Yu. [Institute of Applied Physics of the Russian Academy of Sciences, 46 Ul' yanov Str., 603950 Nizhny Novgorod (Russian Federation); Nizhny Novgorod State University, 23 Gagarin Ave., 603950 Nizhny Novgorod (Russian Federation); Zotova, I. V.; Sergeev, A. S.; Zheleznov, I. V.; Samsonov, S. V.; Mishakin, S. V. [Institute of Applied Physics of the Russian Academy of Sciences, 46 Ul' yanov Str., 603950 Nizhny Novgorod (Russian Federation)

    2015-11-15

    A time-domain self consistent theory of a gyrotron traveling wave tube with a helically corrugated operating waveguide has been developed. Based on this model, the process of short pulse amplification was studied in regimes of grazing and intersection of the dispersion curves of the electromagnetic wave and the electron beam. In the first case, the possibility of amplification without pulse form distortion was demonstrated for the pulse spectrum width of the order of the gain bandwidth. In the second case, when the electrons' axial velocity was smaller than the wave's group velocity, it was shown that the slippage of the incident signal with respect to the electron beam provides feeding of the signal by “fresh” electrons without initial modulation. As a result, the amplitude of the output pulse can exceed the amplitude of its saturated value for the case of the grazing regime, and, for optimal parameters, the peak output power can be even larger than the kinetic power of the electron beam.

  15. Experimental results of the 140 GHz, 1 MW long-pulse gyrotron for W7-X

    Science.gov (United States)

    Koppenburg, K.; Arnold, A.; Borie, E.; Dammertz, G.; Giguet, E.; Heidinger, R.; Illy, S.; Kuntze, M.; Le Cloarec, G.; Legrand, F.; Leonhardt, W.; Lievin, C.; Neffe, G.; Piosczyk, B.; Schmid, M.; Thumm, M.

    2003-02-01

    Gyrotrons at high frequency with high output power are mainly developed for microwave heating and current drive in plasmas for thermonuclear fusion. For the stellarator Wendelstein 7-X now under construction at IPP Greifswald, Germany, a 10 MW ECRH system is foreseen. A 1 MW, 140 GHz long-pulse gyrotron has been designed and a pre-prototype (Maquette) has been constructed and tested in an European collaboration between FZK Karlsruhe, CRPP Lausanne, IPF Suttgart, IPP Greifswald, CEA Cadarache and TED Vélizy [1]. The cylindrical cavity is designed for operating in the TE28,8 mode. It is a standard tapered cavity with linear input downtaper and a non-linear uptaper. The diameter of the cylindrical part is 40.96 mm. The transitions between tapers and straight section are smoothly rounded to avoid mode conversion. The TE28,8-cavity mode is transformed to a Gaussian TEM0,0 output mode by a mode converter consisting of a rippled-wall waveguide launcher followed by a three mirror system. The output window uses a single, edge cooled CVD-diamond disk with an outer diameter of 106 mm, a window aperture of 88 mm and a thickness of 1.8 mm corresponding to four half wavelengths. The collector is at ground potential, and a depression voltage for energy recovery can be applied to the cavity and to the first two mirrors. Additional normal-conducting coils are employed to the collector in order to produce an axial magnetic field for sweeping the electron beam with a frequency of 7 Hz. A temperature limited magnetron injection gun without intermediate anode ( diode type ) is used. In short pulse operation at the design current of 40 A an output power of 1 MW could be achieved for an accelerating voltage of 82 kV without depression voltage and with a depression voltage of 25 kV an output power of 1.15 MW at an accelerating voltage of 84 kV has been measured. For these values an efficiency of 49% was obtained. At constant accelerating voltages, the output power did not change up to

  16. Gyrotron-driven high current ECR ion source for boron-neutron capture therapy neutron generator

    Energy Technology Data Exchange (ETDEWEB)

    Skalyga, V., E-mail: skalyga.vadim@gmail.com [Institute of Applied Physics, RAS, 46 Ul’yanova st., 603950 Nizhny Novgorod (Russian Federation); Lobachevsky State University of Nizhny Novgorod (UNN), 23 Gagarina st., 603950 Nizhny Novgorod (Russian Federation); Izotov, I.; Golubev, S.; Razin, S. [Institute of Applied Physics, RAS, 46 Ul’yanova st., 603950 Nizhny Novgorod (Russian Federation); Sidorov, A. [Institute of Applied Physics, RAS, 46 Ul’yanova st., 603950 Nizhny Novgorod (Russian Federation); Lobachevsky State University of Nizhny Novgorod (UNN), 23 Gagarina st., 603950 Nizhny Novgorod (Russian Federation); Maslennikova, A. [Lobachevsky State University of Nizhny Novgorod (UNN), 23 Gagarina st., 603950 Nizhny Novgorod (Russian Federation); Nizhny Novgorod State Medical Academy, 10/1 Minina Sq., 603005 Nizhny Novgorod (Russian Federation); Volovecky, A. [Lobachevsky State University of Nizhny Novgorod (UNN), 23 Gagarina st., 603950 Nizhny Novgorod (Russian Federation); Kalvas, T.; Koivisto, H.; Tarvainen, O. [University of Jyvaskyla, Department of Physics, PO Box 35 (YFL), 40500 Jyväskylä (Finland)

    2014-12-21

    Boron-neutron capture therapy (BNCT) is a perspective treatment method for radiation resistant tumors. Unfortunately its development is strongly held back by a several physical and medical problems. Neutron sources for BNCT currently are limited to nuclear reactors and accelerators. For wide spread of BNCT investigations more compact and cheap neutron source would be much more preferable. In present paper an approach for compact D–D neutron generator creation based on a high current ECR ion source is suggested. Results on dense proton beams production are presented. A possibility of ion beams formation with current density up to 600 mA/cm{sup 2} is demonstrated. Estimations based on obtained experimental results show that neutron target bombarded by such deuteron beams would theoretically yield a neutron flux density up to 6·10{sup 10} cm{sup −2}/s. Thus, neutron generator based on a high-current deuteron ECR source with a powerful plasma heating by gyrotron radiation could fulfill the BNCT requirements significantly lower price, smaller size and ease of operation in comparison with existing reactors and accelerators.

  17. Use of the Iowa Model of Research in Practice as a Curriculum Framework for Doctor of Nursing Practice (DNP) Project Completion.

    Science.gov (United States)

    Lloyd, Susan T; D'Errico, Ellen; Bristol, Shirley T

    2016-01-01

    Doctoral education requires academic motivation and persistence on the part of nursing students; commitment to the process is essential and should be linked to programmatic structure. Programmatic issues in doctor of nursing practice (DNP) programs may be barriers to completion of the final project and lead to attrition. A large, private health care university developed an infrastructure for the DNP curriculum and final project utilizing the Iowa Model of Research in Practice. The purpose was to ensure competency fulfillment, retention and timely completion, and implementation of evidence-based practice and translation science utilizing a leadership approach. The program has experienced a high completion rate to date.

  18. Long-lived states of magnetically equivalent spins populated by dissolution-DNP and revealed by enzymatic reactions.

    Science.gov (United States)

    Bornet, Aurélien; Ji, Xiao; Mammoli, Daniele; Vuichoud, Basile; Milani, Jonas; Bodenhausen, Geoffrey; Jannin, Sami

    2014-12-15

    Hyperpolarization by dissolution dynamic nuclear polarization (D-DNP) offers a way of enhancing NMR signals by up to five orders of magnitude in metabolites and other small molecules. Nevertheless, the lifetime of hyperpolarization is inexorably limited, as it decays toward thermal equilibrium with the nuclear spin-lattice relaxation time. This lifetime can be extended by storing the hyperpolarization in the form of long-lived states (LLS) that are immune to most dominant relaxation mechanisms. Levitt and co-workers have shown how LLS can be prepared for a pair of inequivalent spins by D-DNP. Here, we demonstrate that this approach can also be applied to magnetically equivalent pairs of spins such as the two protons of fumarate, which can have very long LLS lifetimes. As in the case of para-hydrogen, these hyperpolarized equivalent LLS (HELLS) are not magnetically active. However, a chemical reaction such as the enzymatic conversion of fumarate into malate can break the magnetic equivalence and reveal intense NMR signals.

  19. Long-Lived States of Magnetically Equivalent Spins Populated by Dissolution-DNP and Revealed by Enzymatic Reactions**

    Science.gov (United States)

    Bornet, Aurélien; Ji, Xiao; Mammoli, Daniele; Vuichoud, Basile; Milani, Jonas; Bodenhausen, Geoffrey; Jannin, Sami

    2014-01-01

    Hyperpolarization by dissolution dynamic nuclear polarization (d-DNP) offers a way of enhancing NMR signals by up to five orders of magnitude in metabolites and other small molecules. Nevertheless, the lifetime of hyperpolarization is inexorably limited, as it decays toward thermal equilibrium with the nuclear spin-lattice relaxation time. This lifetime can be extended by storing the hyperpolarization in the form of long-lived states (LLS) that are immune to most dominant relaxation mechanisms. Levitt and co-workers have shown how LLS can be prepared for a pair of inequivalent spins by d-DNP. Here, we demonstrate that this approach can also be applied to magnetically equivalent pairs of spins such as the two protons of fumarate, which can have very long LLS lifetimes. As in the case of para-hydrogen, these hyperpolarized equivalent LLS (HELLS) are not magnetically active. However, a chemical reaction such as the enzymatic conversion of fumarate into malate can break the magnetic equivalence and reveal intense NMR signals. PMID:25346515

  20. Suppression of the Oscillatory Modes of a Space Charge in the Magnetron Injection Guns of Technological Gyrotrons

    Science.gov (United States)

    Glyavin, M. Yu.; Kuntsevich, A. D.; Manuilov, V. N.

    2015-01-01

    We present the results of based on the PIC method numerical simulation of the dynamic processes of trapping of electrons into the adiabatic trap of a technological gyrotron for different configurations of the electric and magnetic fields in the electron beam formation region. The electrode geometry providing a low reflection coefficient of the magnetic mirror to suppress oscillatory modes in the space-charge cloud and ensure the stability of the electron beam with a high fraction of oscillatory energy in such a system has been found.

  1. Real-time DNP NMR observations of acetic acid uptake, intracellular acidification, and of consequences for glycolysis and alcoholic fermentation in yeast.

    Science.gov (United States)

    Jensen, Pernille R; Karlsson, Magnus; Lerche, Mathilde H; Meier, Sebastian

    2013-09-27

    Uptake and upshot in vivo: Straightforward methods that permit the real-time observation of organic acid influx, intracellular acidification, and concomitant effects on cellular-reaction networks are crucial for improved bioprocess monitoring and control. Herein, dynamic nuclear polarization (DNP) NMR is used to observe acetate influx, ensuing intracellular acidification and the metabolic consequences on alcoholic fermentation and glycolysis in living cells.

  2. Neutron generator for BNCT based on high current ECR ion source with gyrotron plasma heating.

    Science.gov (United States)

    Skalyga, V; Izotov, I; Golubev, S; Razin, S; Sidorov, A; Maslennikova, A; Volovecky, A; Kalvas, T; Koivisto, H; Tarvainen, O

    2015-12-01

    BNCT development nowadays is constrained by a progress in neutron sources design. Creation of a cheap and compact intense neutron source would significantly simplify trial treatments avoiding use of expensive and complicated nuclear reactors and accelerators. D-D or D-T neutron generator is one of alternative types of such sources for. A so-called high current quasi-gasdynamic ECR ion source with plasma heating by millimeter wave gyrotron radiation is suggested to be used in a scheme of D-D neutron generator in the present work. Ion source of that type was developed in the Institute of Applied Physics of Russian Academy of Sciences (Nizhny Novgorod, Russia). It can produce deuteron ion beams with current density up to 700-800 mA/cm(2). Generation of the neutron flux with density at the level of 7-8·10(10) s(-1) cm(-2) at the target surface could be obtained in case of TiD2 target bombardment with deuteron beam accelerated to 100 keV. Estimations show that it is enough for formation of epithermal neutron flux with density higher than 10(9) s(-1) cm(-2) suitable for BNCT. Important advantage of described approach is absence of Tritium in the scheme. First experiments performed in pulsed regime with 300 mA, 45 kV deuteron beam directed to D2O target demonstrated 10(9) s(-1) neutron flux. This value corresponds to theoretical estimations and proofs prospects of neutron generator development based on high current quasi-gasdynamic ECR ion source.

  3. Three-Dimensional Numerical Simulation of a 30-GHz Gyrotron Resonator With an Explicit High-Order Discontinuous-Galerkin-Based Parallel Particle-In-Cell Method

    DEFF Research Database (Denmark)

    Stock, Andreas; Neudorfer, Jonathan; Riedlinger, Marc;

    2012-01-01

    Fast design codes for the simulation of the particle–field interaction in the interior of gyrotron resonators are available. They procure their rapidity by making strong physical simplifications and approximations, which are not known to be valid for many variations of the geometry and the operat...

  4. Free-electron masers vs. gyrotrons: prospects for high-power sources at millimeter and submillimeter wavelengths

    Energy Technology Data Exchange (ETDEWEB)

    Thumm, Manfred E-mail: manfred.thumm@ihm.fzk.de

    2002-05-01

    The possible applications of high-power millimeter (mm) and sub-mm waves from free-electron masers (FEMs) and gyro-devices span a wide range of technologies. The plasma physics community has already taken advantage of recent advances in applying high-power mm waves generated by long pulse or continuous wave (CW) gyrotron oscillators and short pulse very high-power FEMs in the areas of RF-plasma production, heating, non-inductive current drive, plasma stabilization and active plasma diagnostics for magnetic confinement thermonuclear fusion research, such as electron cyclotron resonance heating (28-170 GHz), electron cyclotron current drive , collective Thomson scattering , microwave transmission and heat-wave propagation experiments. Continuously frequency tunable FEMs could widen these fields of applications. Another important application of CW gyrotrons is industrial materials processing, e.g. sintering of high-performance functional and structural nanostructured ceramics. Sub-mm wave sources are employed in high-frequency broadband electron paramagnetic resonance and other types of spectroscopy. Future applications which await the development of novel high-power FEM amplifiers and gyro-amplifiers include high-resolution radar ranging and imaging in atmospheric and planetary science as well as deep-space and specialized satellite communications and RF drivers for next-generation high-gradient linear accelerators (supercolliders). The present paper reviews the state-of-the-art and future prospects of these recent applications of gyro-devices and FEMs and compares their specific advantages.

  5. Insight into the Supramolecular Architecture of Intact Diatom Biosilica from DNP-Supported Solid-State NMR Spectroscopy.

    Science.gov (United States)

    Jantschke, Anne; Koers, Eline; Mance, Deni; Weingarth, Markus; Brunner, Eike; Baldus, Marc

    2015-12-01

    Diatom biosilica is an inorganic/organic hybrid with interesting properties. The molecular architecture of the organic material at the atomic and nanometer scale has so far remained unknown, in particular for intact biosilica. A DNP-supported ssNMR approach assisted by microscopy, MS, and MD simulations was applied to study the structural organization of intact biosilica. For the first time, the secondary structure elements of tightly biosilica-associated native proteins in diatom biosilica were characterized in situ. Our data suggest that these proteins are rich in a limited set of amino acids and adopt a mixture of random-coil and β-strand conformations. Furthermore, biosilica-associated long-chain polyamines and carbohydrates were characterized, thereby leading to a model for the supramolecular organization of intact biosilica.

  6. Effects of INH, DNP, 2,4-D and CMU on the photosynthetic activity of barley and maize plants; Efecto de cuatro inhibidores metabolicos (INH, DNP, 2, 4-D y CMU) sobre la actividad fotosintetica de plantular de cebada (Hordeum vulgare L.) y Maiz (Zea mais L.)

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez, J.; Prieto, M. P.

    1979-07-01

    Determinations of the rate of photosynthesis were made in barley and maize leaves treated with INH, DNP, 2,4-D or CMU. 1 ppm of the chemicals in nutritive solutions was absorbed by roots during 24 or 48 hours in both dark and light conditions. After this period, photosynthetic activity, compensation point and 14{sup C}O{sub 2} assimilation were determined. Results show that INH increases the rate of photosynthesis, DNP and 2,4-D do not alter it sensibly and CMU acts as a strong inhibitor of photosynthesis. Some possible applications for ths obtention of labelled compounds by biosynthesis are discussed. (Author) 87 refs.

  7. 60 GHz gyrotron development program. Final report, April 1979-June 1984

    Energy Technology Data Exchange (ETDEWEB)

    Shively, J.F.; Bier, R.E.; Caplan, M.; Cheng, M.K.; Choi, E.; Conner, C.C.; Craig, L.J.; Evans, S.J.; Evers, S.J.; Felch, K.L.

    1986-01-01

    The original objective of this program was to develop a microwave amplifier or oscillator capable of producing 200 kW CW power output at 110 GHz. The use of cyclotron resonance interaction was pursued, and the design phases of this effort are discussed. Later, however, the program's objective was changed to develop a family of oscillators capable of producing 200 kw of peak output power at 60 GHz. Gyrotron behavior studies were performed at 28 GHz to obtain generic design information as quickly as possible. The first experimental device at 60 GHz produced over 200 kw of peak power at a pulse duration of 20 ..mu..s. Heating problems and mode interference were encountered. The second experimental tube incorporated an optimized gun location but also suffered from mode interference. The third experimental tube included modifications that reduced mode interference. It demonstrated 200 kw of peak output at 100 ms pulse duration. The fourth experimental tube, which used an older rf circuit design but in a CW configuration, produced 71.5 kW CW. The fifth experimental tube incorporated a thinner double-disc output window which improved window bandwidth and reduced window loss. This tube also incorporated modifications to the drift tunnel and cavity coupling, which had proven successful in the third experimental pulse tube tests. It produced 123 kW of CW output power at 60 GHz rf load coolant boiling and tube window failure terminated the tests. A new waterload was designed and constructed, and alternative window designs were explored. A secondary task of developing a 56 GHz CW tube produced in excess of 100 kW CW at this alternate frequency. Testing of the sixth experimental tube resulted in operation at CW output power in the range of 200 to 206 kW for an hour. Output mode purity of the seventh experimental tube was measured at 95% TE/sub 02/. The tube was operated for about forty-five minutes with CW power output over 200 kW.

  8. Programación de un sistema de adquisición de datos utilizando el sistema embebido DNP/1110; Programming of a data acquisition system using the embedded system dil/netpc DNP/1110

    Directory of Open Access Journals (Sweden)

    Josnier Ramos Guardarrama

    2011-06-01

    Full Text Available En el trabajo se presenta una alternativa económica de un sistema de adquisición de datos (SAD basado en el kit dedesarrollo DIL/NetPC DNP/1110, el cual esta formado por un microcontrolador de INTEL SA-1110 StrongARM quetrabaja a 206 MHz y que tiene incorporado un controlador de Ethernet que permite desarrollar aplicaciones con el usode la red. El sistema embebido dispone de un sistema operativo Linux, kernel versión 2.4.18. Como parte del diseñose incorporan de forma compacta los elementos necesarios para que el SAD sea capaz de muestrear ocho señalescapturadas simultáneamente. De particular interés resulta la programación, presentándose dos variantes de solución:cuando se ejecuta la aplicación con un software que corre en el área de usuario y cuando el control se logra con unmódulo del sistema operativo específico para el trabajo del SAD. El sistema está soportado por la integración desoftware libre y propietario.  This work focuses on an economic alternative for developing a data acquisition system (DAS which is made up forThe DIL/NetPC DNP/1110, which provides a very compact Intel 206 MHz SA-1110 StrongARM-based low powerembedded controller with TCP/IP stack and web server for high-speed embedded networking applications. Theembedded system has an operating system Linux, kernel version 2.4.18. The built data acquisition system has thenecessary elements to take charge of governing the sampling process of eight signals. Of particular interest it is theprogramming, being presented two solution variants: when the application is executed with software that it runs inuser's area and when the control is achieved with a specific build module of the operating system for the work of theDAS.. The system is supported by the integration of free software and property software.

  9. Programación de un sistema de adquisición de datos utilizando el sistema embebido DNP/1110;Programming of a data acquisition system using the embedded system dil/netpc DNP/1110

    Directory of Open Access Journals (Sweden)

    Josnier Ramos - Guardarrama,et al.

    2011-06-01

    Full Text Available En el trabajo se presenta una alternativa económica de un sistema de adquisición de datos (SAD basado en el kit de desarrollo DIL/NetPC DNP/1110, el cual esta formado por un microcontrolador de INTEL SA-1110 StrongARM que trabaja a 206 MHz y que tiene incorporado un controlador de Ethernet que permite desarrollar aplicaciones con el uso de la red. El sistema embebido dispone de un sistema operativo Linux, kernel versión 2.4.18. Como parte del diseño se incorporan de forma compacta los elementos necesarios para que el SAD sea capaz de muestrear ocho señales capturadas simultáneamente. De particular interés resulta la programación, presentándose dos variantes de solución: cuando se ejecuta la aplicación con un software que corre en el área de usuario y cuando el control se logra con un módulo del sistema operativo específico para el trabajo del SAD. El sistema está soportado por la integración de software libre y propietario.This work focuses on an economic alternative for developing a data acquisition system (DAS which is made up for The DIL/NetPC DNP/1110, which provides a very compact Intel 206 MHz SA-1110 StrongARM-based low power embedded controller with TCP/IP stack and web server for high-speed embedded networking applications. The embedded system has an operating system Linux, kernel version 2.4.18. The built data acquisition system has the necessary elements to take charge of governing the sampling process of eight signals. Of particular interest it is the programming, being presented two solution variants: when the application is executed with software that it runs in user's area and when the control is achieved with a specific build module of the operating system for the work of the DAS.. The system is supported by the integration of free software and property software.

  10. Study on the Before Cavity Interaction in a Second Harmonic Gyrotron Using 3D CFDTD PIC Simulations

    Science.gov (United States)

    Lin, M. C.; Illy, S.; Thumm, M.; Jelonnek, J.

    2016-10-01

    A computational study on before cavity interaction (BCI) in a 28 GHz second harmonic (SH) gryotron for industrial applications has been performed using a 3-D conformal finite-difference time-domain (CFDTD) particle-in-cell (PIC) method. On the contrary to the after cavity interaction (ACI), i.e. beam wave interaction in the non-linear uptaper after the cavity, which has been widely investigated, the BCI, i.e. beam wave interaction in the non-linear downtaper before the cavity connected to the beam tunnel with an entrance, is less noticed and discussed. Usually the BCI might be considered easy to be eliminated. However, this is not always the case. As the SH gyrotron had been designed for SH TE12 mode operation, the first harmonic (FH) plays the main competition. In the 3-D CFDTD PIC simulations, a port boundary has been employed for the gyro-beam entrance of the gyrotron cavity instead of a metallic short one which is not reflecting a realistic situation as an FH backward wave oscillation (BWO) is competing with the desired SH generation. A numerical instability has been found and identified as a failure of the entrance port boundary caused by an evanescent wave or mode conversion. This indicates the entrance and downtaper are not fully cut-off for some oscillations. A further study shows that the undesired oscillation is the FH TE11 BWO mode concentrated around the beam tunnel entrance and downtaper. A mitigation strategy has been found to suppress this undesired BCI and avoid possible damage to the gun region.

  11. Study on the After Cavity Interaction in a 140 GHz Gyrotron Using 3D CFDTD PIC Simulations

    Science.gov (United States)

    Lin, M. C.; Illy, S.; Avramidis, K.; Thumm, M.; Jelonnek, J.

    2016-10-01

    A computational study on after cavity interaction (ACI) in a 140 GHz gryotron for fusion research has been performed using a 3-D conformal finite-difference time-domain (CFDTD) particle-in-cell (PIC) method. The ACI, i.e. beam wave interaction in the non-linear uptaper after the cavity has attracted a lot of attention and been widely investigated in recent years. In a dynamic ACI, a TE mode is excited by the electron beam at the same frequency as in the cavity, and the same mode is also interacting with the spent electron beam at a different frequency in the non-linear uptaper after the cavity while in a static ACI, a mode interacts with the beam both at the cavity and at the uptaper, but at the same frequency. A previous study on the dynamic ACI on a 140 GHz gyrotron has concluded that more advanced numerical simulations such as particle-in-cell (PIC) modeling should be employed to study or confirm the dynamic ACI in addition to using trajectory codes. In this work, we use a 3-D full wave time domain simulation based on the CFDTD PIC method to include the rippled-wall launcher of the quasi-optical output coupler into the simulations which breaks the axial symmetry of the original model employing a symmetric one. A preliminary simulation result has confirmed the dynamic ACI effect in this 140 GHz gyrotron in good agreement with the former study. A realistic launcher will be included in the model for studying the dynamic ACI and compared with the homogenous one.

  12. Real-Time DNP NMR Observations of Acetic Acid Uptake, Intracellular Acidification, and of Consequences for Glycolysis and Alcoholic Fermentation in Yeast

    DEFF Research Database (Denmark)

    Jensen, Pernille Rose; Karlsson, Magnus; Lerche, Mathilde Hauge

    2013-01-01

    Uptake and upshot in vivo: Straightforward methods that permit the real-time observation of organic acid influx, intracellular acidification, and concomitant effects on cellular-reaction networks are crucial for improved bioprocess monitoring and control (see scheme). Herein, dynamic nuclear pola...... polarization (DNP) NMR is used to observe acetate influx, ensuing intracellular acidification and the metabolic consequences on alcoholic fermentation and glycolysis in living cells....

  13. THE ROLE OF THREE WILD ANIMALS IN THE DISTRIBUTION OF PREFERRED FORAGE PLANTS IN THE DINDER NATIONAL PARK (D.N.P SUDAN

    Directory of Open Access Journals (Sweden)

    F. A. MOHAMMED

    2013-07-01

    Full Text Available This study was conducted in Dinder National Park (D.N.P. of Sudan during the dry season (March, April and May. Waterbuck (Kobus defassa, warthog (Phacochoerus aethiopicus and Tiang (Damaliscus korrigum in D.N.P were chosen for this study. Seeds of Acacia nubica, Acacia seyal and Piliostigma reticulatum recovered from the fecal samples of waterbuck showed a highly increased rate of germination above the control. Acacia polycantha and Sesbania sesban showed decreased rate of germination below the control. The germination rate of Acacia siberiana showed no positive effect (zero versus the control. The germination rate of the seeds of Ziziphus-spina-christi remained more or less above the control (53% and 50%, respectively. The germination of seeds of Ziziphus spina-christi from fecal samples of warthog showed higher increased rate of germination. The results of this study confirmed that the three wild herbivores are grazers, but they shift their diets towards forbs, woody plants and fruits of leguminous trees during the dry season. Waterbuck, Tiang and Warthog they depended on the plant diversely around water collecting places in the pank (Mayas for their diets, but they selected other plant species from the surrounding. Also this study provides the information regarding food habits and feed requirements of these wild herbivores. Such information might help in the management of the habitat (Mayas and the protection and sustainability of wild herbivores in D.N.P.

  14. Control of the Superconducting Magnets current Power Supplies of the TJ-II Gyrotrons; Control de las Fuentes de Corriente de las Bobinas Superconductoras de los Girotrones del TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Ros, A.; Fernandez, A.; Tolkachev, A.; Catalan, G.

    2006-07-01

    The TJ-II ECRH heating system consists of two gyrotrons, which can deliver a maximum power of 300 kW at a frequency of 53.2 GHz. Another 28 GHz gyrotron is going to be used in the Bernstein waves heating system. In order to get the required frequency, the gyrotrons need and homogeneous magnetic field of several tesla, which is generated by a superconducting coil field by a current source. This document describes the current source control as well as the high precision ammeters control. These ammeters measure the current in the superconducting coils. The user interface and the programming of the control system are described. The communication between devices is also explained. (author) 9 Refs.

  15. Effects of INH, DNP, 2, 4-D and CMU on the sugar content of the barley and maize leaves; Efecto de cuatro inhibidores metabolicos (INH, DNP, 2, 3-D y CMU) sobre el contenido en azucares de hohas de cebada (Hordeum vulgare L.) y Maiz (Zea mais L.)

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez, J.; Sancho, P.

    1979-07-01

    1 ppm of the chemicals in nutritive solution was absorbed by barley and maize roots during 24 and 48 hours in dark or light conditioners in order to determine the best conditions. for the obtention of labelled sugars with high specific activity. Results show that the highest specific activity was obtained In maize plants treated with DNP for 24 hours in dark conditions. (Author) 51 refs.

  16. Chemical-shift-resolved ¹⁹F NMR spectroscopy between 13.5 and 135 MHz: Overhauser-DNP-enhanced diagonal suppressed correlation spectroscopy.

    Science.gov (United States)

    George, Christy; Chandrakumar, Narayanan

    2014-08-01

    Overhauser-DNP-enhanced homonuclear 2D (19)F correlation spectroscopy with diagonal suppression is presented for small molecules in the solution state at moderate fields. Multi-frequency, multi-radical studies demonstrate that these relatively low-field experiments may be operated with sensitivity rivalling that of standard 200-1000 MHz NMR spectroscopy. Structural information is accessible without a sensitivity penalty, and diagonal suppressed 2D NMR correlations emerge despite the general lack of multiplet resolution in the 1D ODNP spectra. This powerful general approach avoids the rather stiff excitation, detection, and other special requirements of high-field (19)F NMR spectroscopy.

  17. A high-order particle-in-cell method for low density plasma flow and the simulation of gyrotron resonator devices

    Energy Technology Data Exchange (ETDEWEB)

    Stock, Andreas

    2013-04-26

    Within this thesis a parallelized, transient, three-dimensional, high-order discontinuous Galerkin Particle-in-Cell solver is developed and used to simulate the resonant cavity of a gyrotron. The high-order discontinuous Galerkin approach - a Finite-Element type method - provides a fast and efficient algorithm to numerically solve Maxwell's equations used within this thesis. Besides its outstanding dissipation and dispersion properties, the discontinuous Galerkin approach easily allows for using unstructured grids, as required to simulate complex-shaped engineering devices. The discontinuous Galerkin approach approximates a wavelength with significantly less degrees of freedom compared to other methods, e.g. Finite Difference methods. Furthermore, the parallelization capabilities of the discontinuous Galerkin framework are excellent due to the very local dependencies between the elements. These properties are essential for the efficient numerical treatment of the Vlasov-Maxwell system with the Particle-in-Cell method. This system describes the self-consistent interaction of charged particles and the electromagnetic field. As central application within this thesis gyrotron resonators are simulated with the discontinuous Galerkin Particle-in-Cell method on high-performance-computers. The gyrotron is a high-power millimeter wave source, used for the electron cyclotron resonance heating of magnetically confined fusion plasma, e.g. in the Wendelstein 7-X experimental fusion-reactor. Compared to state-of-the-art simulation tools used for the design of gyrotron resonators the Particle-in-Cell method does not use any significant physically simplifications w.r.t. the modelling of the particle-field-interaction, the geometry and the wave-spectrum. Hence, it is the method of choice for validation of current simulation tools being restricted by these simplifications. So far, the Particle-in-Cell method was restricted to be used for demonstration calculations only, because

  18. Instrumentation for solid-state dynamic nuclear polarization with magic angle spinning NMR

    Science.gov (United States)

    Rosay, Melanie; Blank, Monica; Engelke, Frank

    2016-03-01

    Advances in dynamic nuclear polarization (DNP) instrumentation and methodology have been key factors in the recent growth of solid-state DNP NMR applications. We review the current state of the art of solid-state DNP NMR instrumentation primarily based on available commercial platforms. We start with a general system overview, including options for microwave sources and DNP NMR probes, and then focus on specific developments for DNP at 100 K with magic angle spinning (MAS). Gyrotron microwave sources, passive components to transmit microwaves, the DNP MAS probe, a cooling device for low-temperature MAS, and sample preparation procedures including radicals for DNP are considered.

  19. Comparative analysis of fourth-harmonic multiplying gyrotron traveling-wave amplifiers operating at different frequency multiplications

    Science.gov (United States)

    Yeh, Y. S.; Kao, W. J.; Li, L. J.; Guo, Y. W.

    2017-01-01

    The harmonic multiplying operation in a gyrotron traveling-wave amplifier (gyro-TWA) permits magnetic field reduction and frequency multiplication. This study presents a comparative analysis of fourth-harmonic multiplying gyro-TWAs with three schemes of operation. An improved mode-selective circuit using circular waveguides with various radii provides the rejection points within the range of operating frequencies to suppress the competing modes of gyro-TWAs. The simulated results reveal that gyro-TWAs are the most susceptible to the fundamental-harmonic TE11 competing mode, regardless of the operating scheme, and that the mode-selective circuit can provide an attenuation of more than 20 dB to suppress the competing modes. The amplification of the waves in a gyro-TWA depends on the lengths of the sections, and the simulated results show that the gain increases for all schemes, as the length of the lossy section or the length of the copper section increases. All schemes exhibit nearly the same saturated output powers and bandwidths; however, the saturated gain of the scheme at a high frequency multiplication ratio is less than that of the scheme at a low frequency multiplication ratio. Extensive numerical calculations of power and gain scaling are conducted for all schemes.

  20. A Stable 0.2-THz Coaxial-Waveguide Gyrotron Traveling-Wave-Tube Amplifier with Distributed Losses

    Science.gov (United States)

    Hung, C. L.; Yeh, Y. S.; Chang, T. H.; Fang, R. S.

    2016-08-01

    For high-power operation, a THz gyrotron traveling-wave-tube (gyro-TWT) amplifier must operate in a high-order waveguide mode to enlarge the transverse dimension of an interaction waveguide. However, a gyro-TWT amplifier operating in a high-order waveguide mode is susceptible to spurious oscillations. To improve the device stability, in this study, we investigate the possibility of using a coaxial waveguide with distributed losses as the interaction structure. For the same required attenuation, all threatening oscillating modes can be suppressed using different combinations of losses of inner and outer cylinders. This provides flexibility in designing distributed losses when considering the ohmic loading of the interaction structure. We predict that the 0.2-THz gyro-TWT can stably produce a peak power of 14 kW with an efficiency of 23 %, a 3-dB bandwidth of 3.5 GHz, and a saturated gain of 50 dB for a 20-kV 3-A electron beam with a 5 % velocity spread and 1.0 velocity ratio.

  1. Gyrotron whispering gallery mode coupler with a mode conversion reflector for exciting a circular symmetric uniform phase RF beam in a corrugated waveguide

    Energy Technology Data Exchange (ETDEWEB)

    Neilson, Jeffrey M.

    2017-07-25

    A cylindrical waveguide with a mode converter transforms a whispering gallery mode from a gyrotron cylindrical waveguide with a helical cut launch edge to a quasi-Gaussian beam suitable for conveyance through a corrugated waveguide. This quasi-Gaussian beam is radiated away from the waveguide using a spiral cut launch edge, which is in close proximity to a first mode converting reflector. The first mode converting reflector is coupled to a second mode converting reflector which provides an output free-space HE11 mode wave suitable for direct coupling into a corrugated waveguide. The radiated beam produced at the output of the second mode converting reflector is substantially circular.

  2. W波段三次谐波回旋管实验研究%Experimental study on W-band third-harmonic gyrotron

    Institute of Scientific and Technical Information of China (English)

    孙迪敏; 胡鹏; 马国武; 陈洪斌; 孟凡宝; 陈怀璧

    2013-01-01

    It is the first time that the W-band,third harmonic gyrotron radiation is observed in China.The operation mode of the third harmonic gyrotron is TE61.The magnetic field is 1.2 T and the frequency is 94.86 GHz.When beam current changes from 1.6 A to 4.4 A,keeping the anode voltage 45 kV,the third harmonic radiation can be observed.The largest output power is 4.9 kW,and the efficiency is about 3%.%国内首次成功进行W波段三次谐波回旋管实验.回旋管工作模式为TE61,磁场1.2T,采用拍频法测定工作频率为94.86 GHz.电子束电压为45 kV时,电流1.6~4.4A范围内都观测到了三次谐波振荡信号.采用焦热计测定最大输出功率4.9 kW,效率约3%.

  3. 三次谐波光子带隙谐振腔回旋管%Third-Harmonic of Photonic-Band-Gap Cavity Gyrotron

    Institute of Scientific and Technical Information of China (English)

    夏玉玺; 唐昌建

    2011-01-01

    Effective coupling of interaction between high-order electromagnetic mode gyrotron oscillator and high-effective electron cyclotron mode is achieved through the analysis of mode selection function of the photonic band gap cavity(PBGC).After band characteristics of the PBGC are investigated,TE34-mode is set as working mode and the mode competition is suppressed successfully.The concept of equivalent radius of PBGC gyrotron is established,self-consistent nonlinear theory and related computer simulation program are completed.Studies have shown that TE34-mode can interact with third electronic cyclotron harmonics effectively,the coupling frequency of which is 130.5GHz,and meanwhile it reduces the required magnetic field greatly.The parameters are optimized when many physical factors are taken into account,and a third-harmonic PBGC gyrotron oscillator with voltage 430KV,current 35A,output power 1.75MW and interaction efficiency 11.5% are obtained consequently.%分析了光子晶体谐振腔(PBGC)的模式选择功能,实现PBGC回旋管振荡器高阶电磁模与高次电子回旋模的有效耦合。通过对PBGC禁带特性的分析,定出了工作模式TE34模,并成功抑制了模式竞争。文中建立了PBGC回旋管的等效半径的概念,完成了自洽非线性理论和相关的计算机数值模拟程序。研究发现TE34模能有效地与电子的3次回旋谐波相互作用,其耦合频率为130.5GHz,并极大地降低了对工作磁场的要求。在考虑诸多物理因素影响的情况下,对该3次谐波PBGC回旋管振荡器进行了参数优化研究,得到了参数为:电压430kV、

  4. Automated Microwave Complex on the Basis of a Continuous-Wave Gyrotron with an Operating Frequency of 263 GHz and an Output Power of 1 kW

    Science.gov (United States)

    Glyavin, M. Yu.; Morozkin, M. V.; Tsvetkov, A. I.; Lubyako, L. V.; Golubiatnikov, G. Yu.; Kuftin, A. N.; Zapevalov, V. E.; V. Kholoptsev, V.; Eremeev, A. G.; Sedov, A. S.; Malygin, V. I.; Chirkov, A. V.; Fokin, A. P.; Sokolov, E. V.; Denisov, G. G.

    2016-02-01

    We study experimentally the automated microwave complex for microwave spectroscopy and diagnostics of various media, which was developed at the Institute of Applied Physics of the Russian Academy of Sciences in cooperation with GYCOM Ltd. on the basis of a gyrotron with a frequency of 263 GHz and operated at the first gyrofrequency harmonic. In the process of the experiments, a controllable output power of 0 .1 -1 kW was achieved with an efficiency of up to 17 % in the continuous-wave generation regime. The measured radiation spectrum with a relative width of about 10 -6 and the frequency values measured at various parameters of the device are presented. The results of measuring the parameters of the wave beam, which was formed by a built-in quasioptical converter, as well as the data obtained by measuring the heat loss in the cavity and the vacuum output window are analyzed.

  5. Rapid Sintering of Silica Xerogel Ceramic Derived from Sago Waste Ash Using Sub-millimeter Wave Heating with a 300 GHz CW Gyrotron

    Science.gov (United States)

    Aripin, Haji; Mitsudo, Seitaro; Sudiana, I. Nyoman; Tani, Shinji; Sako, Katsuhide; Fujii, Yutaka; Saito, Teruo; Idehara, Toshitaka; Sabchevski, Sliven

    2011-06-01

    In this paper, we present and discuss experimental results from a microwave sintering of a silica-glass ceramic, produced from a silica xerogel extracted from a sago waste ash. As a radiation source for the microwave heating a sub-millimeter wave gyrotron (Gyrotron FU CW I) with an output frequency of 300 GHz has been used. The powders of silica xerogel have been dry pressed and then sintered at temperatures ranging from 300°C to 1500°C. The influence of the sintering temperature on the technological properties such as porosity and bulk density was studied in detail. Furthermore, X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) spectroscopy have been used in order to study the structure of the produced silica glass-ceramics. It has been found that the silica xerogel crystallizes at a temperature of 800°C, which is about 200°C lower than the one observed in the conventional process. The silica xerogel samples sintered by their irradiation with a sub-millimeter wave at 900°C for 18 minutes are fully crystallized into a silica glass-ceramic with a density of about 2.2 g/cm3 and cristobalite as a major crystalline phase. The results obtained in this study allow one to conclude that the microwave sintering with sub-millimeter waves is an appropriate technological process for production of silica glass-ceramics from a silica xerogel and is characterized with such advantages as shorter times of the thermal cycle, lower sintering temperatures and higher quality of the final product.

  6. Effects of DNP on Thermolysis and Combustion Characteristics of CMDB Propellant%DNP对CMDB推进剂燃烧性能及热分解的影响

    Institute of Scientific and Technical Information of China (English)

    齐晓飞; 严启龙; 王晗; 张腊莹

    2009-01-01

    用燃速测试和高压差示扫描量热法(PDSC)试验,研究了N,N-二硝基哌嗪(DNP)逐渐取代改性双基推进剂(CMDB)中的黑索今(RDX)后,对推进剂燃烧性能和热分解特性的影响.研究结果表明,DNP的加入减缓了CMDB推进剂中RDX的热分解反应,使CMDB推进剂燃速降低,压强指数变小,且高压下(12~18 MPa)燃速降低幅度更加明显.当DNP含量增加到20%(DN3)时,DNP的放热分解峰从推进剂主分解放热峰中分离出来,此放热峰在9 MPa下出现肩峰.

  7. Research on Gyrotrons.

    Science.gov (United States)

    1985-04-15

    perpendicular to a magnetic field tics, radar, and far-infrared astronomy. One wit ’ loss in a collisionless plasma.4 It is * such mechanism is the...16 I -3.218 B13 B16 B17 B18 I Big 0.1283 x 10- 𔃻 9.623 I 0.1558 x 10- 11 -0.1431 x 10- ! 0.8756 x 10 - 20 64 Eq.(4.25), the corresponding...10- 1 -0.8546 x 10- 3 I B 15 B16 B17 I Big Big -0.8612 x 10’u j -. 1283 x 10 0.4692 x 10’ " -0.7586 x 10 0.4973 x 10- We may conclude that for

  8. A 0.52THz third harmonic little-orbit gyrotron%小回旋三次谐波0.52THz回旋管

    Institute of Scientific and Technical Information of China (English)

    袁学松; 马春燕; 韩煜; 鄢扬

    2012-01-01

    A third harmonic little-orbit gyrotron oscillator is investigated theoretically in order to develop high power and efficiency terahertz (THz) radiation sources. Mode competition with different electron-beam parameters have been investigated in a 0.52 THz third harmonic TE37 mode gyrotron oscillator, which can generate 3.7kW of RF power at resonant magnetic field 6.98T. In addition, a high magnetic compression ratio magnetron injection gun (MIG) with 65 kV/2. 5 A has been developed. PIC simulation results show that the velocity ratio of electron beam is 1.24, the average beam radius in the cavity is 0.35 mm. The spread of perpendicular and parallel velocities are 6.1% and 6.6% , respectively.%为了发展大功率高效率太赫兹辐射源,对小回旋电子注激励三次谐波太赫兹电子回旋脉塞进行了研究,分析了不同参数情况下的模式竞争.研究结果表明,采用近轴小回旋电子注能够实现三次谐波单模振荡.在此基础上设计了一只0.52 THz、TE37模三次谐波回旋管,数值计算表明,该回旋管在工作磁场为6.98 T下输出功率可以达到3.7 kW.对产生近轴小回旋电子注的高磁压缩比磁控注入式电子光学系统进行的粒子模拟研究结果表明,该电子枪能够产生满足实验要求的65 kV/2.5 A,横纵速度比为1.24,引导中心半径0.35 mm的小回旋电子注,其纵向速度离散6.6%,横向速度离散6.1%.

  9. 170 GHz回旋管电子枪的设计%Design of the magnetron injection gun for 170 GHz gyrotron

    Institute of Scientific and Technical Information of China (English)

    曾旭; 王峨锋; 刘本田; 李志良; 冯进军

    2013-01-01

    A 170 GHz gryotron is an ideal power source for the Electron Cyclotron Resonance Heating (ECRH) in the plasma fusion. The required output power from a single gyrotron tube at 170 GHz operating frequency for ECRH application is 1 MW or more. For generating the high output power,a Magnetron Injection Gun(MIG) which can provide the electron beam with large enough transverse energy for interacting with the transverse filed of the electromagnetic wave is designed. Ideal parameters of the double-anode MIG are obtained according to the adiabatic compression theory and the correlative simulation software. The designed MIG can provide the necessary beams for the 170 GHz fundamental harmonic gyrotron under 80 kV of voltage and 40 A of current with the guidance center radius of 8.27 mm and the pitch factor of 1.5.%  170 GHz回旋管是等离子体核聚变中电子回旋谐振加热的理想功率源,在功率上要求具有1 MW以上的输出功率。要产生这么大的输出功率,就需要具有足够大横向能量的电子注与高频场的横向电场进行互作用,因此要对电子枪进行专门的设计。利用绝热压缩理论及相关的仿真软件对双阳极磁控注入电子枪进行了设计,得到了较好的电子注参数。所设计的电子枪能在工作电压80 kV、工作电流40 A 的条件下为170 GHz 基波回旋管提供所需的回旋电子注,其引导中心半径为8.27 mm,横纵速度比1.5。

  10. 170 GHz兆瓦级同轴回旋振荡管的分析计算%Analysis and calculation of a 170 GHz megawatt-level coaxial gyrotron

    Institute of Scientific and Technical Information of China (English)

    覃觅觅; 罗勇; 杨阔; 黄勇

    2014-01-01

    回旋管是最有希望应用于正在实施的国际热核实验反应堆计划的微波源器件,然而研究设计符合要求的回旋管还存在很多困难需要解决.对170 GHz兆瓦级光滑同轴回旋管的注-波互作用进行了研究.选取模式谱相对稀疏的TE31,12作为工作模式,利用Matlab编制源程序,计算了同轴回旋管的注-波耦合系数、起振电流.在考虑电子速度零散、腔壁电阻率和单模近似的基础上,对光滑同轴谐振腔的优化设计和注-波互作用进行了仿真,给出了磁场、电压、电流和内导体倾角等参量与回旋管效率的关系.结果表明,电压和磁场对回旋管效率影响较大,电子速度零散对回旋管效率影响较小,因而可降低电子枪的设计要求.此外,优化内导体倾角和同轴谐振腔结构参数可提高注-波互作用效率,降低电子速度零散对互作用效率的影响,获得了约50%的电子效率及1.7 MW输出功率.%Gyrotrons are the most promising microwave source devices that can be used in the International Thermonuclear Experimental Reactor, but there are many diffculties to be solved in study and design of gyrotrons to meet the require-ments. In this paper, the beam-wave interactions of a 170 GHz megawatt-level smooth-wall coaxial gyrotron are studied numerically. In order to attain high effciency and stable radiation, TE31,12 mode that lies in a relative sparse spectrum is selected as the operating mode, and the beam-wave coupling coeffcient and start oscillation current are calculated by a set of source codes developed by Matlab. Taking into account the electronic velocity spread and cavity wall resistivity, and based on a single-mode approximation, the optimization design and simulation of beam-wave interaction of a 170 GHz megawatt smooth-wall coaxial gyrotron have been fulfilled. The relationships between effciency and magnetic field, and the voltage, current, taper angle of insert, and other parameters are

  11. Emittance of short-pulsed high-current ion beams formed from the plasma of the electron cyclotron resonance discharge sustained by high-power millimeter-wave gyrotron radiation

    Science.gov (United States)

    Razin, S.; Zorin, V.; Izotov, I.; Sidorov, A.; Skalyga, V.

    2014-02-01

    We present experimental results on measuring the emittance of short-pulsed (≤100 μs) high-current (80-100 mA) ion beams of heavy gases (Nitrogen, Argon) formed from a dense plasma of an ECR source of multiply charged ions (MCI) with quasi-gas-dynamic mode of plasma confinement in a magnetic trap of simple mirror configuration. The discharge was created by a high-power (90 kW) pulsed radiation of a 37.5-GHz gyrotron. The normalized emittance of generated ion beams of 100 mA current was (1.2-1.3) π mm mrad (70% of ions in the beams). Comparing these results with those obtained using a cusp magnetic trap, it was concluded that the structure of the trap magnetic field lines does not exert a decisive influence on the emittance of ion beams in the gas-dynamic ECR source of MCI.

  12. Design cusp electron gun for of 0.6 THz 3rd-harmonic large orbit gyrotron%0.6THz三次谐波大回旋电子枪设计

    Institute of Scientific and Technical Information of China (English)

    马春燕; 胡强; 袁学松; 韩煜; 鄢扬

    2012-01-01

    Large orbit axis-encircling electron beams can provide improved coupling and mode selectivity in a high harmonic terahertz gyrotron. For developing a 0. 6 THz 3rd-harmonic large orbit gyrotron, a cusp gun with a beam voltage of 55 kV and a beam current of 1 A has been designed based on a comparatively simple Pierce-type electron gun and cusp magnetic field. The optimized result shows that the velocity ratio, axial velocity spread and transverse velocity spread are 1. 53, 7. 10% and 3. 39% , respectively.%基于会切磁场的理论模型,采用粒子模拟软件对0.6 THz三次谐波的太赫兹回旋管所需的大回旋电子光学系统进行研究.文中通过大量的模拟计算,分析讨论了不同参数对电子注的横向速度离散、纵向速度离散及横纵速度比的影响,优化了电子光学系统的性能参量,得到符合设计要求且具有工程实际应用的电子枪,该电子枪能够产生55 kV,1A,横向速度离散为3.39%、纵向速度离散为7.10%、横纵速度比为1.53的大回旋电子注.

  13. Determinação de efedrinas em urina por cromatografia em fase gasosa (CG/DNP para o controle da dopagem no esporte Gas chromatographic method for the determination of ephedrines in urine for doping control purposes

    Directory of Open Access Journals (Sweden)

    Paula Rodrigues Garcia

    2005-09-01

    Full Text Available Efedrinas são aminas simpatomiméticas componentes de diversas especialidades farmacêuticas, utilizadas no tratamento de doenças respiratórias devido à sua ação descongestionante e broncodilatora. Atualmente, diversos produtos comercializados como suplementos nutricionais contêm efedrinas e são amplamente utilizados no meio esportivo, com o objetivo de facilitar a queima de gorduras e melhorar o desempenho. Entretanto, o uso indiscriminado destas substâncias pode acarretar série de efeitos tóxicos como hipertensão, taquicardia, cefaléia e tremores. Devido à sua ação psicoestimulante, foram incluídas na lista de substâncias proibidas nas atividades esportivas pelo Comitê Olímpico Internacional (COI e estabelecidas concentrações na urina para o controle da dopagem (efedrina e metilefedrina: 10 µg/mL. O presente trabalho teve como objetivo a validação de um método para quantificação de efedrinas, por cromatografia em fase gasosa acoplada a detetor de nitrogênio/fósforo (CG/DNP, em amostras de urina com a finalidade de controle da dopagem. O método consistiu em extração líquido-líquido e posterior derivação das efedrinas com anidrido trifluoroacético, e demonstrou ser simples e prático, apresentando linearidade nas faixas de concentração estudadas. Amostras de urina de voluntários que relataram uso de efedrinas foram submetidas à análise pelo método proposto.Ephedrines are sympathomimetic amines present in many pharmaceutical preparations used in the treatment of respiratory diseases due to their actions against broncospasm and congestion. Nowadays, several products sold as nutritional supplements contain ephedrines and are widely used in a diverse range of sports as weight loss aids and enhancement of athletic performance. However, the abuse of ephedrines may lead to a number of adverse effects including hypertension, headache, tachycardia and seizure. Due to their CNS stimulating action, ephedrines are

  14. 太阳光下TiO2/SiO2气凝胶复合光催化剂光催化降解2,4-二硝基酚%Solar photocatalytic degradation of 2,4-dinitrophenol (DNP) using TiO2/SiO2 aerogel composite photocatalysts

    Institute of Scientific and Technical Information of China (English)

    梁文珍; 王慧龙; 姜文凤

    2011-01-01

    以正硅酸乙酯(TEOS)为原料,采用溶胶-凝胶法(so1-ge1)和常压干燥法制备SiO2气凝胶,在此基础上,将硅气凝胶掺杂进入TiO2溶胶中制备出TiO2/SiO2气凝胶复合光催化剂.利用xRD、TEM和FI-IR等技术对其晶型、形貌和结构进行表征,研究了太阳光下TiO2/SiO2气凝胶复合光催化荆对2,4-二硝基苯酚(DNP)的光催化降解性能.结果表明,500℃焙烧后的TiO2/SiO2气凝胶复合材料对DNP溶液的吸附率和光降解率明显优于纯TiO2样品,加入7.5%(质量分数)SiO2气凝胶的复合光催化剂表现出最佳的光催化活性.根据Langmuir-Hinshelwood方程,在低浓度下光催化降解DNP的反应符合一级反应动力学.%Silica aerogels were synthesized by sol-gel technique at ambient pressure using tetraethyl orthosilioate. The TiO2/silica aerogel composite photocatalysts were prepared by doping silica aerogel into the nanosized TiO2 particles. The microstrueture and morphology of the photocatnlysts were characterized by XRD, TEM and FT-IR techniques. The photocatalytic degradation of 2,4-dinitrophenol (DNP) was investigated by using this novel TiO2/silica aerogel composite photocatalyst under solar light irradiation. The results showed that the highest photocatalytic activity was obtained when the composite sample was calcined at 500℃ with an optimal silica aerogel content of 7.5% (wt.). Compared to the pure TiO2 catalyst, the TiO2/silica aerogel composite photocatalyst showed much higher adsorption capacity and photocatalytic degradation efficiency for DNP. The photocatalytic reaction is discussed in terms of Langmuir-Hinshelwood model and it followed pseudo-first order kinetics at low concentration.

  15. Simulation study for measurement of horizontal wind profiles in the polar stratosphere and mesosphere using ground-based observations of ozone and carbon monoxide lines in the 230-250 GHz region

    Science.gov (United States)

    Newnham, David A.; Ford, George P.; Moffat-Griffin, Tracy; Pumphrey, Hugh C.

    2016-07-01

    Meteorological and atmospheric models are being extended up to 80 km altitude but there are very few observing techniques that can measure stratospheric-mesospheric winds at altitudes between 20 and 80 km to verify model datasets. Here we demonstrate the feasibility of horizontal wind profile measurements using ground-based passive millimetre-wave spectroradiometric observations of ozone lines centred at 231.28, 249.79, and 249.96 GHz. Vertical profiles of horizontal winds are retrieved from forward and inverse modelling simulations of the line-of-sight Doppler-shifted atmospheric emission lines above Halley station (75°37' S, 26°14' W), Antarctica. For a radiometer with a system temperature of 1400 K and 30 kHz spectral resolution observing the ozone 231.28 GHz line we estimate that 12 h zonal and meridional wind profiles could be determined over the altitude range 25-74 km in winter, and 28-66 km in summer. Height-dependent measurement uncertainties are in the range 3-8 m s-1 and vertical resolution ˜ 8-16 km. Under optimum observing conditions at Halley a temporal resolution of 1.5 h for measuring either zonal or meridional winds is possible, reducing to 0.5 h for a radiometer with a 700 K system temperature. Combining observations of the 231.28 GHz ozone line and the 230.54 GHz carbon monoxide line gives additional altitude coverage at 85 ± 12 km. The effects of clear-sky seasonal mean winter/summer conditions, zenith angle of the received atmospheric emission, and spectrometer frequency resolution on the altitude coverage, measurement uncertainty, and height and time resolution of the retrieved wind profiles have been determined.

  16. Profile of telomerase and telomerase RNA expression in nasopharyngeal carcinogen esis of rats induced by N, N'dinitrosopiperazine (DNP)%鼻咽上皮癌变过程中端粒酶活性和端粒酶RNA的表达

    Institute of Scientific and Technical Information of China (English)

    唐发清; 蒋海鹰; 段朝军; 谌兵来; 荆照政; 吴尚辉

    2001-01-01

    目的研究二亚硝基哌嗪(DNP)诱发大鼠鼻咽癌变过程中端粒酶的表达规律。方法以DNP诱导大鼠鼻咽癌;用PCR-ELISA 和Nested RT-PCR检测DNP诱导大鼠鼻咽癌变不同阶段端粒酶活性和端粒酶RNA的表达,同时作病理形态学检测。结果 DNP诱导大鼠鼻咽癌变过程中,端粒酶活性不断升高,端粒酶的变化与鼻咽癌变呈正相关,而且端粒酶中RNA表达先于端粒酶的表达。在大鼠鼻咽上皮细胞异型增生阶段即出现端粒酶的激活和端粒酶RNA的表达。结论化学致癌物DNP诱导细胞癌变端粒酶的激活,且端粒酶的激活和端粒酶RNA的表达是鼻咽上皮癌变的早发事件,与鼻咽癌的发生、发展有关。%Objective  To investigate the profile of telomerase and telomerase RNA expression in nasopharyngeal carcinogenesis (NPC) induced by N,N'dinitrosopi perazine (DNP) and examined histolofically. Methods Nasopharyngeal carcinomas o f rats were induced by DNP and examined histologically. PCR-ELISA and nested R T -PCR were used to assay telomerase and telomerase RNA expression at different s tages in the nasopharyngeal tissues of rats. Results  During the carcinogenesis p rocess, telomerase activity increased along with the formation of a nasopharynge al carcinoma. Telomerase expression was positively related with nasopharyngeal c arcinogenesis. Telomerase RNA expression was present and did not change during t he NPC process. Expression of telomease RNA was earlier than telomerase activat ion. Telomerase activation and telomerase RNA expressin were also detected in th e pre-cancerous nasopharyngeal lesions. Conclusion  Telomerase activation may p articipate in the onset and progression of NPC, and is an early step in NPC.

  17. Anti-DNP IgE 联合 DNFB 诱导免疫性接触性荨麻疹小鼠模型的建立%A Mouse Model of Immunological Contact Urticaria by Anti-dinitrophenol IgE Combined with 2, 4-Dinitrofluorobenzene

    Institute of Scientific and Technical Information of China (English)

    李润祥; 冯承恩; 梁碧华; 田歆; 朱慧兰

    2015-01-01

    Objective:To establish an animal model of immunological contact urticaria of mice . Methods:Thirty-six BALB/c mice were randomly divided into 3 groups.The mice of model group were injected with anti-dinitrophenol IgE monoclonal antibody by tail vein .The mice of control groups were injected with anti-dinitrophenol IgE monoclonal antibody or normal saline by tail vein . After 24 hours, 2, 4 -dinitrofluorobenzene ( DNFB) was painted on the ears of model group and physiological saline was painted on the ears of control groups respectively .Mice were observed within 14 days of the wheal, pruritus, ear thickness, serum levels of IgE, IL-4 and IFN-γ, hista-mine and skin pathology .Results:Wheals were seen on the mice of all of mode group .Compared with control groups , scratching number , ear thickness and serum levels of histamine of the mice of model group were increased significantly (P0.05 ) .Conclusion:A mouse model of immunological contact urticaria would be quickly and reliably established by induction of anti -dini-trophenol IgE monoclonal antibody and DNFB .%目的:利用抗-二硝基苯酚(anti-DNP)IgE单克隆抗体和2,4-二硝基氟苯(DNFB)建立免疫性接触性荨麻疹小鼠模型。方法:将36只BALB/c小鼠随机分成3组,每组12只:造模组小鼠尾静脉注射anti-DNP IgE单克隆抗体,24 h后在小鼠双耳涂DNFB进行激发;单纯IgE组仅注射anti-DNP IgE单克隆抗体,空白对照组小鼠注射生理盐水,均在24 h后在小鼠双耳涂生理盐水。观察小鼠在14 d内的风团、瘙痒、耳朵肿胀度情况,并检测血清中IgE、IL-4、IFN-γ、组胺水平和皮肤病理。结果:造模组全部小鼠产生风团;与其它两组相比,造模组小鼠搔抓次数、耳朵肿胀度及组胺血清水平均明显升高(P值均<0.01),但三组间IgE、IL-4、IFN-γ水平无明显差异(P>0.05)。结论:静脉注射anti-DNP IgE单克隆抗体联合外用DNFB可快速、可

  18. Rapid frequency step-switching in submillimeter wave gyrotrons (Gyrotrons FU III and FU IV)

    OpenAIRE

    Idehara, T.; Shimizu, Y; Ogawa, I.; Tatsukawa, T.; BRAND, G.F.

    1999-01-01

    The rapid frequency step-switching between two nearby cyclotron fundamental modes achieved by switching beam energy is analyzed by a computer simulation and demonstrated experimentally. More dramatic frequency switching between a fundamental mode and a second harmonic mode is analyzed and demonstrated experimentally.

  19. Study of molecular interactions with 13C DNP-NMR.

    Science.gov (United States)

    Lerche, Mathilde H; Meier, Sebastian; Jensen, Pernille R; Baumann, Herbert; Petersen, Bent O; Karlsson, Magnus; Duus, Jens Ø; Ardenkjaer-Larsen, Jan H

    2010-03-01

    NMR spectroscopy is an established, versatile technique for the detection of molecular interactions, even when these interactions are weak. Signal enhancement by several orders of magnitude through dynamic nuclear polarization alleviates several practical limitations of NMR-based interaction studies. This enhanced non-equilibrium polarization contributes sensitivity for the detection of molecular interactions in a single NMR transient. We show that direct (13)C NMR ligand binding studies at natural isotopic abundance of (13)C gets feasible in this way. Resultant screens are easy to interpret and can be performed at (13)C concentrations below muM. In addition to such ligand-detected studies of molecular interaction, ligand binding can be assessed and quantified with enzymatic assays that employ hyperpolarized substrates at varying enzyme inhibitor concentrations. The physical labeling of nuclear spins by hyperpolarization thus provides the opportunity to devise fast novel in vitro experiments with low material requirement and without the need for synthetic modifications of target or ligands.

  20. Frequency swept microwaves for hyperfine decoupling and time domain dynamic nuclear polarization.

    Science.gov (United States)

    Hoff, Daniel E M; Albert, Brice J; Saliba, Edward P; Scott, Faith J; Choi, Eric J; Mardini, Michael; Barnes, Alexander B

    2015-11-01

    Hyperfine decoupling and pulsed dynamic nuclear polarization (DNP) are promising techniques to improve high field DNP NMR. We explore experimental and theoretical considerations to implement them with magic angle spinning (MAS). Microwave field simulations using the high frequency structural simulator (HFSS) software suite are performed to characterize the inhomogeneous phase independent microwave field throughout a 198GHz MAS DNP probe. Our calculations show that a microwave power input of 17W is required to generate an average EPR nutation frequency of 0.84MHz. We also present a detailed calculation of microwave heating from the HFSS parameters and find that 7.1% of the incident microwave power contributes to dielectric sample heating. Voltage tunable gyrotron oscillators are proposed as a class of frequency agile microwave sources to generate microwave frequency sweeps required for the frequency modulated cross effect, electron spin inversions, and hyperfine decoupling. Electron spin inversions of stable organic radicals are simulated with SPINEVOLUTION using the inhomogeneous microwave fields calculated by HFSS. We calculate an electron spin inversion efficiency of 56% at a spinning frequency of 5kHz. Finally, we demonstrate gyrotron acceleration potentials required to generate swept microwave frequency profiles for the frequency modulated cross effect and electron spin inversions. Copyright © 2015 Elsevier Inc. All rights reserved.

  1. Investigations of Cavity Designs for a High Power Gyrotron.

    Science.gov (United States)

    1982-05-20

    WGM in a coaxial resonator, one has to work with very small annulus spacing, thus making electron beam alignment difficult and, more importantly...are shown in Table 1. In order to operate with a low order mode, one has to work with a small annulus spacing (making beam alignment critical) and

  2. The DNP/MPH Dual Degree: An Innovative Graduate Education Program for Advanced Public Health Nursing.

    Science.gov (United States)

    Shaw, Kathy; Harpin, Scott; Steinke, Geraldine; Stember, Marilyn; Krajicek, Marilyn

    2017-03-01

    Strong professional priorities, evolving Affordable Care Act requirements, and a significantly limited public health nursing workforce prompted the University of Colorado College of Nursing to collaborate with the School of Public Health to implement one of the first Doctor of Nursing Practice/Master of Public Health dual degree programs in the nation. Federal grant funding supported the development, implementation, and evaluation of this unique post-baccalaureate dual degree program, for which there were no roadmaps, models, or best practices to follow. Several key issues emerged that serve as lessons learned in creating a new, novel higher education pathway for Advanced Public Health Nursing. This paper highlights two of those: (1) marketing, admission, and matriculation across two programs, and (2) enhancing curricula through distance coursework and interprofessional education. When collaboration with a school of public health is possible, the Doctor of Nursing Practice/Master of Public Health dual degree is an efficient way to prepare public health nurses' with the highest level of public health knowledge, practice, and leadership expertise. © 2016 Wiley Periodicals, Inc.

  3. Experimental Determination of Physical Properties of DNGU, TNBA, LLM-105, HK-56, and DNP

    Science.gov (United States)

    2016-09-01

    TGA thermogravimetric analysis TNBA 2,4,6-trinitro-3-bromoanisole TNT 2, 4, 6-trinitrotoluene UV ultraviolet Approved for public release...for public release; distribution unlimited. 13. SUPPLEMENTARY NOTES 14. ABSTRACT Chemical analysis of 5 energetic materials—1,4...differential scanning calorimetry; and thermogravimetric analysis traces. The hydrolysis half-life time for DNGU is also reported. 15. SUBJECT TERMS

  4. Large dose hyperpolarized water with dissolution-DNP at high magnetic field

    Science.gov (United States)

    Lipsø, Kasper Wigh; Bowen, Sean; Rybalko, Oleksandr; Ardenkjær-Larsen, Jan Henrik

    2017-01-01

    We demonstrate a method for the preparation of hyperpolarized water by dissolution Dynamic Nuclear Polarization at high magnetic field. Protons were polarized at 6.7 T and 1.1 K to >70% with frequency modulated microwave irradiation at 188G Hz. 97.2 ± 0.7% of the radical was extracted from the sample in the dissolution in a two-phase system. 16 ± 1 mL of 5.0 M 1H in D2O with a polarization of 13.0 ± 0.9% in the liquid state was obtained, corresponding to an enhancement factor of 4000 ± 300 compared to the thermal equilibrium at 9.4 T and 293 K. A longitudinal relaxation time constant of 16 ± 1 s was measured. The sample was polarized and dissolved in a fluid path compatible with clinical polarizers. The volume of hyperpolarized water produced by this method enables angiography and perfusion measurements in large animals, as well as NMR experiments for studies of e.g. proton exchange and polarization transfer to other nuclei.

  5. Overcoming the Tension: Building Effective DNP-PhD Faculty Teams.

    Science.gov (United States)

    Staffileno, Beth A; Murphy, Marcia Pencak; Carlson, Elizabeth

    2016-01-01

    A shift in terminal degrees held by nursing faculty is occurring as the number of doctor of nursing practice graduates continues to rise. This change has resulted in some uncertainty and tension. At the same time, there is a pressing need for collaboration among doctoral-prepared nurse leaders to improve care and outcomes for the population. An intellectual community that nurtures learning for all members serves as a blueprint for moving toward a professoriate that engages both doctor of nursing practice- and doctor of philosophy-prepared faculty and enhances scholarly activities, research, and teaching productivity. Although this may seem like an intangible concept, strategic steps can be instituted to promote positive relationships and transform the academic culture into an environment that fosters a vibrant intellectual life for all faculty members. Therefore, the purpose of this article is to propose that doctoral faculty embrace a transformational change to promote a community of scholars who serve as stewards of the discipline. Specific strategies for transforming the culture and building an intellectual community are described. Copyright © 2016 Elsevier Inc. All rights reserved.

  6. Large dose hyperpolarized water with dissolution-DNP at high magnetic field

    DEFF Research Database (Denmark)

    Lipsø, Hans Kasper Wigh; Bowen, Sean; Rybalko, Oleksandr

    2016-01-01

    We demonstrate a method for the preparation of hyperpolarized water by dissolution Dynamic Nuclear Polarization at high magnetic field. Protons were polarized at 6.7T and 1.1K to >70% with frequency modulated microwave irradiation at 188GHz. 97.2±0.7% of the radical was extracted from the sample...... in the dissolution in a two-phase system. 16±1mL of 5.0M (1)H in D2O with a polarization of 13.0±0.9% in the liquid state was obtained, corresponding to an enhancement factor of 4000±300 compared to the thermal equilibrium at 9.4T and 293K. A longitudinal relaxation time constant of 16±1s was measured. The sample...

  7. Microfluorometric assessment of the DNA-DNP complex in human spermatozoa.

    Science.gov (United States)

    Johannisson, E; Jacolino, H

    1987-06-01

    During spermiogenesis, the DNA-nucleoprotein complex undergoes alterations that are reflected in a decreasing capacity for binding DNA-specific dyes, such as ethidium bromide (EB). Human spermatozoa with a low or high capacity for EB binding were depleted of RNA and most nuclear proteins by exposure to RNAse, EDTA and trypsin, with and without additional high salt buffer (HSB) treatment. When treated with RNAse, EDTA and trypsin only, the haploid DNA fluorescence value (calculated from the diploid value of the standard cell population) was found at EB concentrations of 6.5 to 12.5 micrograms/mL. At these EB concentrations, a significantly lower fluorescence was found in the material also treated with HSB, probably reflecting an unwinding of the highly negatively supercoiled DNA loops that are induced by HSB treatment. Maximal fluorescence was not found until a concentration of 50 micrograms EB/mL. This may be due to an overwinding of the DNA by the positive supercoiling caused by EB. The significant difference in EB uptake initially found between the two groups whose spermatozoa showed low and high capacities for EB binding disappeared after removal of the nucleoproteins, suggesting that this compartment of the nucleoprotein-DNA complex is responsible for the different uptakes of EB.

  8. New Radiation Input/Output Systems for Millimeter-Wave Gyrotron Traveling-Wave Tubes

    Science.gov (United States)

    Denisov, G. G.; Bogdashov, A. A.; Gachev, I. G.; Mishakin, S. V.; Samsonov, S. V.

    2016-03-01

    We consider in detail the method allowing one to input and output the microwave radiation produced by an elecrovacuum amplifier through the same barrier window, which was proposed earlier, in the context of its application in a traveling-wave tube based on a waveguide with a helically corrugated surface. Special attention is given to the splitter of differently polarized radiation, and the results of studying this splitter at wavelengths of about 6 and 1 mm theoretically and experimentally are presented.

  9. Klystrons, traveling wave tubes, magnetrons, crossed-field amplifiers, and gyrotrons

    CERN Document Server

    Gilmour, A S

    2011-01-01

    Microwave tubes are vacuum electron devices used for the generation and amplification of radio frequencies in the microwave range. An established technology area, the use of tubes remains essential in the field today for high-power applications. The culmination of the author's 50 years of industry experience, this authoritative resource offers you a thorough understanding of the operations and major classes of microwave tubes.Minimizing the use of advanced mathematics, the book places emphasis on clear qualitative explanations of phenomena. This practical reference serves as an excellent intro

  10. On a Wide-Band Fast Wave Gyrotron Travelling Wave Amplifier.

    Science.gov (United States)

    1980-12-10

    Laboratory Attn: Dr. Paul Tallerico P.O. Box 1663 Los Alamos, New Mexico 87545 (1) Massachusetts Institute of Technology Research Laboratory of...Addressee (1) TFR Group DPH - PFC Attn: Dr. A. Cavallo 92260 Fontenay-auix Roses France (1 ) Thompson C.S.F./DET/ TDH Attn: Dr. G. Mourier 2 Rue

  11. Experimental Examination of the Enhancement of Gyrotron Efficiencies by use of Profiled Magnetic Fields.

    Science.gov (United States)

    1981-02-04

    Alamos, New Mexico 87545 (1) Massachusetts Institute of Technology Research Laboratory of Electronics Attn: Dr. G. Bekefi Bldg. 36, Rm. 36-225 Cambridge...92260 Fontenay-auix Roses France (1) Thompson C.S.F./DET/ TDH Attn: Dr. G. Mourier 2 Rue Latecoere 78140 Velizy Villa conblay France (1) UKAEA Culham Laboratory Attn: Dr. A.C. Riviere Abingdon Oxfordshire United Kingdom 20

  12. Generation and Improvement of the Gyrotron Electron Beam in a Biased Cusp Magnetic Field.

    Science.gov (United States)

    1981-04-23

    3100 Lomita Boulevard Torrance, California 90509 (1) Los Alamos Scientific Laboratory Attn: Dr. Paul Tallerico P.O. Box 1663 Los Alamos, New Mexico ...Roses France (1) Thompson C.S.F./DET/ TDH Attn: Dr. G. Mourier 2 Rue Latecoere 78140 Velizy Villa conblay France (1) UKAEA Cuiham Laboratory Attn: Dr. A.C. Riviere Abingdon Oxfordshire United Kingdom 20

  13. Particle-in-cell (PIC) simulations of beam instabilities in gyrotron beam tunnels

    Energy Technology Data Exchange (ETDEWEB)

    Tran, T.M.; Jost, G.; Appert, K.; Sauter, O. [Ecole Polytechnique Federale, Lausanne (Switzerland). Centre de Recherche en Physique des Plasma (CRPP); Wuthrich, S. [CRAY Research, PATP/PSE, Ecole Polytechnique Federale, Lausanne (Switzerland)

    1995-10-01

    Experimental observations seem to indicate that the beam velocity and energy spreads are larger than those calculated from the electron trajectory codes which do not take into account the effects of beam instabilities. On the other hand, parasitic oscillations of the beam with frequencies close to the electron cyclotron frequency {omega}{sub ce} have been observed experimentally, suggesting the possibility that instabilities can be excited in the beam tunnels and are responsible for the beam degradation. 2D electrostatic and electromagnetic time-dependent PIC codes have been developed to simulate the beam transport in the beam tunnel. The results of extensive parametric runs, using these codes (which were ported on the Cray T3D massively parallel computer), together with the role of the beam instabilities around {omega}{sub ce} on the beam degradation will be reported. (author) 2 figs., 9 refs.

  14. Hyperpolarized [1,4-13C]-Diethylsuccinate: A Potential DNP Substrate for In Vivo Metabolic Imaging

    OpenAIRE

    Billingsley, Kelvin L.; Josan, Sonal; Park, Jae Mo; Tee, Sui Seng; Spielman-Sun, Eleanor; Hurd, Ralph; Mayer, Dirk; Spielman, Daniel

    2014-01-01

    The tricarboxylic acid cycle (TCA) performs an essential role in the regulation of energy and metabolism, and deficiencies in this pathway are commonly correlated with various diseases. However, the development of non-invasive techniques for the assessment of the cycle in vivo has remained challenging. In this work, the applicability of a novel imaging agent, [1,4-13C]-diethylsuccinate, for hyperpolarized 13C metabolic imaging of the TCA cycle was explored. In vivo spectroscopic studies were ...

  15. Measuring absolute spin polarization in dissolution-DNP by Spin PolarimetrY Magnetic Resonance (SPY-MR).

    Science.gov (United States)

    Vuichoud, Basile; Milani, Jonas; Chappuis, Quentin; Bornet, Aurélien; Bodenhausen, Geoffrey; Jannin, Sami

    2015-11-01

    Dynamic nuclear polarization at 1.2 K and 6.7 T allows one to achieve spin temperatures on the order of a few millikelvin, so that the high-temperature approximation (ΔEPolarimetrY Magnetic Resonance (SPY-MR), is illustrated for various pairs of (13)C spins (I, S) in acetate and pyruvate.

  16. Measuring absolute spin polarization in dissolution-DNP by Spin PolarimetrY Magnetic Resonance (SPY-MR)

    Science.gov (United States)

    Vuichoud, Basile; Milani, Jonas; Chappuis, Quentin; Bornet, Aurélien; Bodenhausen, Geoffrey; Jannin, Sami

    2015-11-01

    Dynamic nuclear polarization at 1.2 K and 6.7 T allows one to achieve spin temperatures on the order of a few millikelvin, so that the high-temperature approximation (Δ E spy'), provided perturbations due to second-order (strong coupling) effects are properly taken into account. If spin S is suitably discreet and does not affect the relaxation of spin I, this provides an elegant way of measuring spin polarizations 'on the fly' in a broad range of molecules, thus obviating the need for laborious measurements of signal intensities at thermal equilibrium. The method, dubbed Spin PolarimetrY Magnetic Resonance (SPY-MR), is illustrated for various pairs of 13 C spins (I, S) in acetate and pyruvate.

  17. Offspring of xenogeneically-reconstituted scid scid mice are capable of a primary xenogeneic immune response to DNP-KLH

    NARCIS (Netherlands)

    Greenwood, JD; Bos, NA; Croy, BA

    1996-01-01

    Human peripheral blood leukocyte (PBL) reconstitution of severe combined immunodeficient (SCID) mice has provided a small animal model system (hu-PBL-SCID) useful for the study of the human immune system and disease pathogenesis. Transfer of xenogeneic PBL from donors other than humans has also been

  18. Offspring of xenogeneically-reconstituted scid scid mice are capable of a primary xenogeneic immune response to DNP-KLH

    NARCIS (Netherlands)

    Greenwood, JD; Bos, NA; Croy, BA

    1996-01-01

    Human peripheral blood leukocyte (PBL) reconstitution of severe combined immunodeficient (SCID) mice has provided a small animal model system (hu-PBL-SCID) useful for the study of the human immune system and disease pathogenesis. Transfer of xenogeneic PBL from donors other than humans has also been

  19. Design and Implementation of a 200kW, 28GHz gyrotron system for the Compact Toroidal Hybrid Experiment

    Science.gov (United States)

    Hartwell, G. J.; Knowlton, S. F.; Ennis, D. A.; Maurer, D. A.; Bigelow, T.

    2016-10-01

    The Compact Toroidal Hybrid (CTH) is an l = 2 , m = 5 torsatron/tokamak hybrid (R0 = 0.75 m, ap 0.2 m, and | B | supplement the existing 15 kW klystron system operating at the fundamental frequency; the latter will be used to initially generate the plasma. Ray-tracing calculations that guide the selection of launching position, antenna focal length, and beam-steering characteristics of the ECRH have been performed with the TRAVIS code [ 1 ] . The calculated absorption is up to 95.7% for vertically propagating rays, however, the absorption is more sensitive to magnetic field variations than for a side launch where the field gradient is tokamak-like. The design of the waveguide path and components for the top-launch scenario will be presented. This work is supported by U.S. Department of Energy Grant No. DE-FG02-00ER54610.

  20. Study on statistical breakdown delay time in argon gas using a W-band millimeter-wave gyrotron

    Science.gov (United States)

    Kim, Dongsung; Kim, Sung Gug; Sawant, Ashwini; Yu, Dongho; Choe, MunSeok; Choi, EunMi

    2016-04-01

    In this study, we investigated plasma initiation delay times for argon volume breakdown at the W-band frequency regime. The threshold electric field is defined as the minimum electric field amplitude needed for plasma breakdown at various pressures. The measured statistical delay time showed an excellent agreement with the theoretical Gaussian distribution and the theoretically estimated formative delay time. Also, we demonstrated that the normalized effective electric field as a function of the product of pressure and formative time shows an outstanding agreement to that of 1D particle-in-cell simulation coupled with a Monte Carlo collision model [H. C. Kim and J. P. Verboncoeur, Phys. Plasmas 13, 123506 (2006)].

  1. Effects of Velocity Spread and Wall Resistivity on the Gain and Bandwidth of the Gyrotron Travelling-Wave Amplifier.

    Science.gov (United States)

    1980-11-12

    Scientific Laboratory Attn: Dr. Paul Tallerico P.O. Box 1663 Los Alamos, New Mexico 87545 (1) Massachusetts Institute of Technology Research Laboratory of...No. of Copies Addressee (1) TFR Group DPH - PFC’ Attn: Dr. A. Cavallo 92260 Fontenay-auix Roses France (1) Thompson C.S.F./DET/ TDH Attn: Dr. G

  2. An all MMIC Replacement for Gunn Diode Oscillators Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The objective of this Phase II project is to develop and demonstrate a compact and reliable oscillator technology for the frequency band from 100 ? 250 GHz for use...

  3. Josephson junction spectrum analyzer for millimeter and submillimeter wavelengths

    Energy Technology Data Exchange (ETDEWEB)

    Larkin, S.Y.; Anischenko, S.E.; Khabayev, P.V. [State Research Center, Kiev (Ukraine)

    1994-12-31

    A prototype of the Josephson-effect spectrum analyzer developed for the millimeter-wave band is described. The measurement results for spectra obtained in the frequency band from 50 to 250 GHz are presented.

  4. Strategy-aligned fuzzy approach for market segment evaluation and selection: a modular decision support system by dynamic network process (DNP)

    Science.gov (United States)

    Mohammadi Nasrabadi, Ali; Hosseinpour, Mohammad Hossein; Ebrahimnejad, Sadoullah

    2013-05-01

    In competitive markets, market segmentation is a critical point of business, and it can be used as a generic strategy. In each segment, strategies lead companies to their targets; thus, segment selection and the application of the appropriate strategies over time are very important to achieve successful business. This paper aims to model a strategy-aligned fuzzy approach to market segment evaluation and selection. A modular decision support system (DSS) is developed to select an optimum segment with its appropriate strategies. The suggested DSS has two main modules. The first one is SPACE matrix which indicates the risk of each segment. Also, it determines the long-term strategies. The second module finds the most preferred segment-strategies over time. Dynamic network process is applied to prioritize segment-strategies according to five competitive force factors. There is vagueness in pairwise comparisons, and this vagueness has been modeled using fuzzy concepts. To clarify, an example is illustrated by a case study in Iran's coffee market. The results show that success possibility of segments could be different, and choosing the best ones could help companies to be sure in developing their business. Moreover, changing the priority of strategies over time indicates the importance of long-term planning. This fact has been supported by a case study on strategic priority difference in short- and long-term consideration.

  5. Toxicology Study No. S.0024589d 15, Human Cell Line Activation Test of the Novel Energetic, 3,4 -Dinitropyrazole (DNP)

    Science.gov (United States)

    2016-04-01

    CO2 . Cells for the assay had not been in culture for more than 30 passages or 60 days. Prior to passage or test plating, cell density was...The following plots were captured for each sample: 2-dimensional plot of forward and side scatter, 2-dimensional dot plot of FITC vs PI and a...or 30,000 total hits on the PI channel. For each sample, the geometric mean fluorescence intensity (MFI) was captured for all hits and live/viable

  6. Initial result of collective Thomson scattering using 77 GHz gyrotron for bulk and tail ion diagnostics in the Large Helical Device

    Energy Technology Data Exchange (ETDEWEB)

    Nishiura, M; Kubo, S; Tanaka, K; Shimozuma, T; Mutoh, T; Kawahata, K; Watari, T [National Institute for Fusion Science, 322-6 Oroshi, Toki, Gifu 509-5292 (Japan); Tamura, N [Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601 (Japan); Saito, T; Tatematsu, Y [FIR FU, University of Fukui, 3-9-1 Bunkyo, Fukui, 910-8507 (Japan); Notake, T, E-mail: nishiura@nifs.ac.j [RIKEN, 519-1399 Aoba, Aramaki, Aoba-ku, Sendai, 980-0845 (Japan)

    2010-05-01

    The collective Thomson scattering (CTS) technique has been utilized with the backscattering configuration in the collective scattering regime to diagnose the velocity distribution functions in the Large Helical Device (LHD). The receiver was equipped with 16 channels and the first test has been carried out using the eight channels for scattered radiation and these channels cover a few GHz frequency shift from the 76.95 GHz probe beam. During the discharge, the electron density and temperature at the central region of the LHD are 1x10{sup 19}m{sup -3}, and 1.0 keV, respectively. The probing beam with rectangular wave modulation is injected by 50 Hz in order to be distinct from the background electron cyclotron emission (ECE). The scattered radiation is resolved successfully at each channel of CTS receiver system. The detected signals of bulk ion and electron components are by a factor of 10 {approx} 10{sup 2} larger than the background ECE signal. We found that the measured spectra are in reasonably agreement with the theoretical spectra calculated by using the reliable measured electron temperature and density for input parameters. The CTS receiver system will be improved to obtain more accurate velocity distributions in high temperature plasmas.

  7. A versatile and modular quasi optics-based 200 GHz dual dynamic nuclear polarization and electron paramagnetic resonance instrument

    Science.gov (United States)

    Siaw, Ting Ann; Leavesley, Alisa; Lund, Alicia; Kaminker, Ilia; Han, Songi

    2016-03-01

    Solid-state dynamic nuclear polarization (DNP) at higher magnetic fields (>3 T) and cryogenic temperatures (∼2-90 K) has gained enormous interest and seen major technological advances as an NMR signal enhancing technique. Still, the current state of the art DNP operation is not at a state at which sample and freezing conditions can be rationally chosen and the DNP performance predicted a priori, but relies on purely empirical approaches. An important step towards rational optimization of DNP conditions is to have access to DNP instrumental capabilities to diagnose DNP performance and elucidate DNP mechanisms. The desired diagnoses include the measurement of the "DNP power curve", i.e. the microwave (MW) power dependence of DNP enhancement, the "DNP spectrum", i.e. the MW frequency dependence of DNP enhancement, the electron paramagnetic resonance (EPR) spectrum, and the saturation and spectral diffusion properties of the EPR spectrum upon prolonged MW irradiation typical of continuous wave (CW) DNP, as well as various electron and nuclear spin relaxation parameters. Even basic measurements of these DNP parameters require versatile instrumentation at high magnetic fields not commercially available to date. In this article, we describe the detailed design of such a DNP instrument, powered by a solid-state MW source that is tunable between 193 and 201 GHz and outputs up to 140 mW of MW power. The quality and pathway of the transmitted and reflected MWs is controlled by a quasi-optics (QO) bridge and a corrugated waveguide, where the latter couples the MW from an open-space QO bridge to the sample located inside the superconducting magnet and vice versa. Crucially, the versatility of the solid-state MW source enables the automated acquisition of frequency swept DNP spectra, DNP power curves, the diagnosis of MW power and transmission, and frequency swept continuous wave (CW) and pulsed EPR experiments. The flexibility of the DNP instrument centered around the QO MW

  8. Radio and Millimeter Observations of the COSMOS Field

    OpenAIRE

    Schinnerer, E.; Bertoldi, F.; Carilli, C.L.; Smolčić, V.; Scoville, N.; K. Menten; Voss, H.; Blain, A; Lutz, D.

    2007-01-01

    The Cosmic Evolution Survey (COSMOS) targets an equatorial two square degree field covering the full electromagnetic spectrum. Here we present first results from observations of the COSMOS field in the millimeter and centimeter regime done with the IRAM 30m/MAMBO array and NRAO's Very Large Array (VLA) at 250GHz and 1.4GHz, respectively.

  9. Comparison of the Performance and Capabilities of Femtocell versus Wi-Fi Networks

    Science.gov (United States)

    2012-09-01

    identify its “neighborhood.” If a femtocell sees that it is in an unauthorized area it can disable itself or notify the provider. Due to the...Hewlett-Packard HP G62 Notebook PC Microprocessor: 2.50GHz VISION Technology from AMD with AMD Turion II Dual-Core Mobile Processor N530 Memory: 4GB

  10. Spectral Dynamics of the Fem

    NARCIS (Netherlands)

    Eecen, P. J.; Tulupov, A. V.; Schep, T. J.

    1995-01-01

    The FOM Fusion FEM project involves the construction and operation of a 1-MW, 100 ms pulse, rapidly tunable FEM in the 130-250 GHz range for fusion applications. The undulator is a novel step-tapered undulator, consisting of two sections with different strengths and lengths and equal periodicities,

  11. Effect of the drift gap between the undulator sections on the operation of the Fusion-FEM

    NARCIS (Netherlands)

    van der Geer, C. A. J.; Militsyn, B. L.; Bongers, W. A.; Bratman, V. L.; Denisov, G. G.; Manintveld, P.; Savilov, A. V.; Varfolomeev, A. A.; Verhoeven, A. G. A.; Urbanus, W. H.

    2000-01-01

    The 'Fusion-FEM' is a free electron MASER based on an electrostatic accelerator. An electron beam of 12 A, 1.35-2 MeV is injected into a step-tapered undulator to generate 1 MW of radiation in the range 130-250 GHz. The undulator is built from two sections with different field strength

  12. Investigation of Multifrequency Generation in the Fem

    NARCIS (Netherlands)

    Eecen, P. J.; Tulupov, A. V.; Schep, T. J.

    1994-01-01

    The FOM Fusion FEM project involves the construction and operation of a 1-MW, 100 ms pulse, rapid tunable FEM in the 130-250 GHz range for fusion applications. The undulator of the FEM consists of two sections with different strengths and different lengths separated by a gap without undulator field.

  13. Synthesis of Insensitive N-2,4-dinitrophenyl-N1-nitroguanidine

    Science.gov (United States)

    Warner, Kirstin F.; Bellitto, Victor J.; Sorensen, Daniel N.; Remmers, Daniel L.

    2016-10-01

    A new, insensitive nitroguanidyl functionalized nitrogen-rich material derived from 2,4-dinitrophenyl derivative was synthesized. N-2,4-dinitrophenyl-N1-nitroguanidine (DNP-NQ) has a crystal density of 1.74 g/cm3. The synthesis and properties of DNP-NQ are described. DNP-NQ was characterized by nuclear magnetic resonance (NMR), infrared spectroscopy, and gas pycnometry. Thermal properties were studied by differential scanning calorimetry (DSC). Results from small-scale sensitivity tests indicate that DNP-NQ is less sensitive than Cyclotrimethylenetrinitramine (RDX). DNP-NQ can be prepared in high yield.

  14. Separation by column chromatography of cells active in delayed-onset hypersensitivities.

    Science.gov (United States)

    Godfrey, H P; Gell, P G

    1976-01-01

    Lymph node cells from guinea-pigs contact sensitive to 1-thiocyamo-2,4-dinitrobenzene have been fractionated by affinity chromatography over modified polyacrylamide beads. Cells mediating lymphokine release in response to active sensitizer were depleted only by chromatography over dinitrophyenyl (DNP) containing substrates and could be specifically eluted with DNP-glycine. DNP rosette-forming cells (RFC) were equally well depleted by chromatography using either DNP or trinitrophenyl containing materials but could not be eluted from the columns by DNP-glycine. While the antigen receptors of cells mediating the release of macrophage agglutination factor in response to DNP-containing antigens and of DNP-RFC were found to be hapten-specific, their specificity was shown to differ using chromatography over trinitrophenyl containing polyacrylamide. PMID:776818

  15. Status of the new multi-frequency ECRH system for ASDEX Upgrade

    DEFF Research Database (Denmark)

    Wagner, D.; Grünwald, G.; Leuterer, F.

    2008-01-01

    Currently, a new multi-frequency ECRH system is under construction at the ASDEX Upgrade tokamak experiment. This system employs, for the first time in a fusion device, multi-frequency gyrotrons, step-tunable in the range 105-140 GHz. The first two gyrotrons, working at 105 and 140 GHz, were...

  16. Development of Mode Conversion Waveguides at KIT

    Directory of Open Access Journals (Sweden)

    Jin Jianbo

    2015-01-01

    Full Text Available The development of mode conversion waveguides (launchers for high power gyrotrons has gone through three stages at KIT. Formerly, harmonically deformed launchers have been used in the series gyrotrons developed for the stellarator W7-X. In 2009, a numerical method for the analysis and synthesis of mirror-line launchers was developed at KIT. Such a launcher with adapted mode-converting mirrors for a 2 MW TE34,19-mode, 170GHz coaxial-cavity gyrotron has been designed and tested, and also a mirror-line launcher for the 1MW EU ITER gyrotron has been designed. Recently, based on the Helmholtz-Kirchhoff integral theorem, a novel numerical method for the synthesis of hybrid-type gyrotron launchers has been developed. As an example, TE32,9 mode launchers operating at 170GHz that have been designed using the three different methods are being compared.

  17. State-of-the-art of high power gyro-devices and free electron masers. Update 2015

    Energy Technology Data Exchange (ETDEWEB)

    Thumm, Manfred [Karlsruher Institut fuer Technologie (KIT), Eggenstein-Leopoldshafen (Germany). Inst. fuer Hochleistungsimpuls- und Mikrowellentechnik, Programm Fusion

    2016-07-01

    Gyrotron oscillators (gyromonotrons) are mainly used as high power millimeter wave sources for electron cyclotron resonance heating (ECRH), electron cyclotron current drive (ECCD), stability control and diagnostics of magnetically confined plasmas for generation of energy by controlled thermonuclear fusion. The maximum pulse length of commercially available 140 GHz, megawatt- class gyrotrons employing synthetic diamond output windows is 30 minutes (CPI and European KIT-CRPP-TED collaboration). The world record parameters of the European megawatt-class 140 GHz gyrotron are: 0.92 MW output power at 30 min. pulse duration, 97.5% Gaussian mode purity and 44% efficiency, employing a single-stage depressed collector (SDC) for energy recovery. A maximum output power of 1.5 MW in 4.0 s pulses at 45% efficiency was generated with the JAEA-TOSHIBA 110 GHz gyrotron. The Japan 170 GHz ITER gyrotron achieved 1 MW, 800 s at 55% efficiency and holds the energy world record of 2.88 GJ (0.8 MW, 60 min.) and the efficiency record of 57% for tubes with an output power of more than 0.5 MW. The Russian 170 GHz ITER gyrotron achieved 0.99 (1.2) MW with a pulse duration of 1000 (100) s and 53 (53) % efficiency. The prototype tube of the European 2 MW, 170 GHz coaxial-cavity gyrotron achieved in short pulses the record power of 2.1 MW at 46% efficiency and 96% Gaussian mode purity. Gyrotrons with pulsed magnet for various short-pulse applications deliver P{sub out}=210 kW with τ=20 μs at frequencies up to 670 GHz (η≅20%), P{sub out}=5.3 kW at 1 THz (η=6.1%), and P{sub out}=0.5 kW at 1.3 THz (η=0.6%). Gyrotron oscillators have also been successfully used in materials processing. Such technological applications require gyrotrons with the following parameters: f ≥ 24 GHz, P{sub out}=4-50 kW, CW, η≥30%. This paper gives an update of the experimental achievements related to the development of high power gyrotron oscillators for long-pulse or CW operation and pulsed gyrotrons for

  18. Membrane and cytoskeletal changes associated with IgE-mediated serotonin release from rat basophilic leukemia cells

    OpenAIRE

    1985-01-01

    Binding of antigen to IgE-receptor complexes on the surface of RBL-2H3 rat basophilic leukemia cells is the first event leading to the release of cellular serotonin, histamine, and other mediators of allergic, asthmatic, and inflammatory responses. We have used dinitrophenol- conjugated bovine serum albumin (DNP-BSA) as well as the fluorescent antigen, DNP-B-phycoerythrin, and the electron-dense antigen, DNP-BSA- gold, to investigate dynamic membrane and cytoskeletal events associated with th...

  19. Cross-suppression of specific immune responses after oral tolerance

    Directory of Open Access Journals (Sweden)

    Nelson M. Vaz

    1981-03-01

    Full Text Available Adult normal inbred mice rendered tolerant to OVA by previous oral exposure do not respond to intraperitonela immunization with DNP-OVA in adjuvant. These tolerant mice also form less DNP-specific antibodies to DNP-KLH when immunized with mixtures of DNP-KLH and DNP-OVA, or less HGG-specific antibodies when immunized with cross-linked conjugates of OVA and HGG. These same procedures increased DNP-specific or HGG-specific responses in non-tolerant control mice. The cross-supperssion was ineffective, however, to inhibit already ongoing antibody responses.Camundongos adultos normais tornados imunologicamente tolerantes a ovoalbumina (OVA por exposição oral não formam anticorpos antidinitrofenil (anti-DNP quando imunizados com DNP-OVA, mas respondem normalmente à DNP-hemocianina (DNO-KLH. Entretanto, a adição de DNP-OVA à injeção de DNP-KLH reduz a formação de anticorpos anti-DNP em animais tolerantes a OVA, mas não em animais normais. Similarmente animais tolerantes à OVA formam menos anticorpos antiglobulina humana (HGG quando imunizados com agregados (por glutaraldeído de OVA e HGG. A tolerância oral e, portanto, capaz de inibir a indução de respostas imunes por um esquema de supressão-cruzada. Esse esquema, no entanto, não foi capaz de inibir respostas imunes já iniciadas.

  20. ACE variants interact with the RAS pathway to confer risk and protection against type 2 diabetic nephropathy

    DEFF Research Database (Denmark)

    Ahluwalia, Tarun Veer Singh; Ahuja, Monica; Rai, Taranjit Singh

    2009-01-01

    , in the present study, we evaluated the association of ACE haplotypes and the interactions of ACE, angiotensinogen (AGT), and angiotensin II receptor type I (AGTR1) gene polymorphisms with DNP in Asian Indians. We genotyped seven variants of the RAS pathway genes (ACE, AGT, and AGTR1) in type 2 diabetic cohorts....../D) was significantly higher in DNP (p AGTR1: A1166C) was higher and associated with increased risk of DNP (235T, p

  1. Dinitrosopiperazine-Mediated Phosphorylated-Proteins Are Involved in Nasopharyngeal Carcinoma Metastasis

    Directory of Open Access Journals (Sweden)

    Gongjun Tan

    2014-11-01

    Full Text Available N,N'-dinitrosopiperazine (DNP with organ specificity for nasopharyngeal epithelium, is involved in nasopharyngeal carcinoma (NPC metastasis, though its mechanism is unclear. To reveal the pathogenesis of DNP-induced metastasis, immunoprecipitation was used to identify DNP-mediated phosphoproteins. DNP-mediated NPC cell line (6-10B motility and invasion was confirmed. Twenty-six phosphoproteins were increased at least 1.5-fold following DNP exposure. Changes in the expression levels of selected phosphoproteins were verified by Western-blotting analysis. DNP treatment altered the phosphorylation of ezrin (threonine 567, vimentin (serine 55, stathmin (serine 25 and STAT3 (serine 727. Furthermore, it was shown that DNP-dependent metastasis is mediated in part through ezrin at threonine 567, as DNP-mediated metastasis was decreased when threonine 567 of ezrin was mutated. Strikingly, NPC metastatic tumors exhibited a higher expression of phosphorylated-ezrin at threonine 567 than the primary tumors. These findings provide novel insight into DNP-induced NPC metastasis and may contribute to a better understanding of the metastatic mechanisms of NPC tumors.

  2. Photothermal therapy combined with dinitrophenyl hapten for the treatment of late stage malignant melanoma

    Science.gov (United States)

    Li, Xiaosong; Du, Nan; Li, Haijun; Long, Shan; Chen, Dianjun; Zhou, Feifan; Xu, Yuanyuan; Wang, Fuli; Chen, Wei R.

    2017-02-01

    To evaluate the efficacy and safety of photothermal with dinitrophenyl hapten (DNP) for patients with malignant melanoma (MM), Patients with pathology confirmed stage III or IV MM were enrolled. Seventy-two patients were randomized into two groups, DNP alone group (n=36) and DNP plus photothermal therapy group (n=36). The results showed that the patients in the combination treatment group had longer median progression-free survival time (19.0m vs. 12.0m, p=0.007). No severe adverse events were observed in both groups. Thus, the combination of photothermal therapy and DNP maybe a new therapeutic strategy for patients with advanced MM.

  3. On the adjuvant effect of aluminum hydroxide for mice

    Directory of Open Access Journals (Sweden)

    Nelson M. Vaz

    1981-03-01

    Full Text Available Linear relationships were found between the dose of A1(OH3 adjuvant and the titer of anti-OVA antibodies formed by BDF1 mice. Mice immunized with OVA, DNP-KLH and then boosted with DNP-OVA formed anti-DNP antibodies only when A1(OH3 was added to the injection of DNP-KLH; addition of A1(OH3 to the priming injection of OVA decreased, rather than increased antibody formation.Há relações lineares entre a dose de Al (OH3 usado como adjuvante imunológico e o título de anticorpos anti-ovoalbumina (anti-OVA formado por camundongos BDFI. Camundongos que receberam OVA, depois uma imunização acessoria com dinitrofenil-hemocianina (DNP-KLH e então uma imunização secundária com DNP-OVA, só formaram anticorpos anti-DNP quando havia Al (OH3 incluído na injeção de DNP-KLH; a inclusão de Al (OH3 na injeção primária de OVA baixou em vez de elevar o título de anticorpos anti-DNP. E sugerido que a ação adjuvante do Al(OH3 se deva a ações sobre linfócitos B.

  4. Preliminary design for a pierce wiggler beamstick and addendum

    Energy Technology Data Exchange (ETDEWEB)

    Pirkle, D.

    1988-05-01

    Lawrence Livermore National Laboratory is developing a fast tunable microwave source for operation at 250 GHz and 10kW peak output power. This report presents the preliminary design of a Pierce gun and solenoid magnet that will be compatible with a Pierce-wiggler electron beam formation system (beamstick). The beamstick will be an appropriate power source for a tunable gyro-BWO at 250 GHz. Figure 1 presents the major components of the Pierce-wiggler beamstick: the electron gun, solenoid, beam tunnel, wiggler, and vacuum valve. Figure 2 shows an artistic conception of how the beamstick will interface with the interaction magnet, modulator and gyro-BWO circuit at MIT. 15 figs.

  5. InGaAs Schottky barrier diode array detector for a real-time compact terahertz line scanner.

    Science.gov (United States)

    Han, Sang-Pil; Ko, Hyunsung; Park, Jeong-Woo; Kim, Namje; Yoon, Young-Jong; Shin, Jun-Hwan; Kim, Dae Yong; Lee, Dong Hun; Park, Kyung Hyun

    2013-11-04

    We present a terahertz (THz) broadband antenna-integrated 1 × 20 InGaAs Schottky barrier diode (SBD) array detector with an average responsivity of 98.5 V/W at a frequency of 250 GHz, which is measured without attaching external amplifiers and Si lenses, and an average noise equivalent power (NEP) of 106.6 pW/√Hz. The 3-dB bandwidth of the SBD detector is also investigated at approximately 180 GHz. For implementing an array-type SBD detector by a simple fabrication process to achieve a high yield, a structure comprising an SiN(x) layer instead of an air bridge between the anode and the cathode is designed. THz line beam imaging using a Gunn diode emitter with a center frequency of 250 GHz and a 1 × 20 SBD array detector is successfully demonstrated.

  6. High order mode beam waveguide for technological medium power millimeter wave applications

    Energy Technology Data Exchange (ETDEWEB)

    Rio, C. del; Gonzalo, R.; Marin, M.; Sorolla, M.; Moebius, A.; Thumm, M. [Universidad Publica de Navarra, Pamplona (Spain)

    1995-12-31

    The use of medium power millimeter CW gyrotrons (10-30 kW and 30-100 GHz) has several potential applications in advanced materials processing. Since a stochastic field distribution in the applicator is desirable no pencil beam is necessary. Then the possibility to couple the circular symmetric gyrotron output to a higher order free space mode can be considered. Beam waveguides based on iterative reflection of such high order beams on properly disigned mirrors opens the possibility to increase the efficiency and to reduce costs of present compact transmission lines in gyrotron technological systems.

  7. Design and operation of the power installation for the TCV ECR additional heating

    Energy Technology Data Exchange (ETDEWEB)

    Fasel, D.; Favre, A.; Goodman, T.; Henderson, M.; Isoz, P.F.; Perez, A.; Tran, M.Q. [Ecole Polytechnique Federale, Lausanne (Switzerland). Centre de Recherche en Physique des Plasma (CRPP); Alex, J. [Thomcast AG, Turgi (Switzerland)

    1996-10-01

    Following a brief introduction to the TCV project, this paper concentrates on the Regulated High Voltage Power Supply (RHVPS) system chosen to supply the nine gyrotrons, distributed in three clusters, that will deliver 4.5 MW of Electron Cyclotron Resonance Heating (ECRH) to TCV plasmas. The configuration of these clusters is described in some detail, including the results of site test both with dummy load (80 kV, 85 A, 2 sec) and the gyrotrons themselves (70 kV, 25 A, 2 sec). Some details are also given of gyrotron auxiliaries, interlock circuitry, control and data acquisition, and integration into TCV control environment. (author) 4 figs., 1 tab., 4 refs.

  8. Plane gyroklinotron at first and third harmonics of cyclotron frequency

    Energy Technology Data Exchange (ETDEWEB)

    Kurayev, A.A.; Lukashonok, D.V.; Sinitsyn, A.K., E-mail: kurayev@bsuir.by, E-mail: timka86@gmail.com [Belarusian State University of Informatics and Radioelectronics, Minsk (Belarus)

    2011-07-01

    The results of gyroklinotron's parameters optimization for efficiency at f = 100 GHz with interaction on first and third harmonics of the cyclotron frequency are presented. The predicted electron gyroklinotron's efficiency reaches 70% on first harmonic and 40% on third harmonic. This is more than in usual gyrotron. Besides in contrast to usual gyrotron the width electron beam on radius of guiding centers of electron orbits in gyroklinotron may considerable exceed working wave length {lambda}. This allows to use in it considerable more power of electron beams EB then in usual gyrotron. (author)

  9. EC-5 fifth international workshop on electron cyclotron emission and electron cyclotron heating

    Energy Technology Data Exchange (ETDEWEB)

    Prater, R.; Lohr, J. [eds.

    1985-12-31

    This report contains papers on the following topics: electron cyclotron emission measurements; electron cyclotron emission theory; electron cyclotron heating; gyrotron development; and ECH systems and waveguide development. These paper have been indexed separately elsewhere. (LSP).

  10. Dispersion of helically corrugated waveguides: Analytical, numerical, and experimental study

    Science.gov (United States)

    Burt, G.; Samsonov, S. V.; Ronald, K.; Denisov, G. G.; Young, A. R.; Bratman, V. L.; Phelps, A. D.; Cross, A. W.; Konoplev, I. V.; He, W.; Thomson, J.; Whyte, C. G.

    2004-10-01

    Helically corrugated waveguides have recently been studied for use in various applications such as interaction regions in gyrotron traveling-wave tubes and gyrotron backward-wave oscillators and as a dispersive medium for passive microwave pulse compression. The paper presents a summary of various methods that can be used for analysis of the wave dispersion of such waveguides. The results obtained from an analytical approach, simulations with the three-dimensional numerical code MAGIC, and cold microwave measurements are analyzed and compared.

  11. Microwave-gated dynamic nuclear polarization

    DEFF Research Database (Denmark)

    Bornet, Aurélien; Pinon, Arthur; Jhajharia, Aditya

    2016-01-01

    Dissolution dynamic nuclear polarization (D-DNP) has become a method of choice to enhance signals in nuclear magnetic resonance (NMR). Recently, we have proposed to combine cross-polarization (CP) with D-DNP to provide high polarization P((13)C) in short build-up times. In this paper, we show...

  12. High Δnp73/TAp73 ratio is associated with poor prognosis in acute promyelocytic leukemia

    NARCIS (Netherlands)

    A.R. Lucena-Araujo (Antonio); H.T. Kim (Haesook T.); C. Thomé (Carolina); R.H. Jacomo (Rafael); R.A. Melo (Raul); H.N.S. Bittencourt (Henrique); R. Pasquini (Ricardo); K. Pagnano (Katia); A.B.F. Glória (Ana Beatriz F.); M. de Lourdes Chauffaille (Maria); M. Athayde (Melina); C.S. Chiattone (Carlos); I. Mito (Ingrid); R. Bendlin (Rodrigo); C. Souza (Carmino); C. Bortolheiro (Cristina); J.L. Coelho-Silva (Juan L.); M. Schrier (Mariëtte); M.S. Tallman (Martin); D. Grimwade (David); A. Ganser (Arnold); N. Berliner (Nancy); R.C. Ribeiro (Raul); F. Lo-Coco (Francesco); B. Löwenberg (Bob); M.A. Sanz (Miguel Angel); E.M. Rego (Eduardo)

    2015-01-01

    textabstractThe TP73 gene transcript is alternatively spliced and translated into the transcriptionally active (TAp73) or inactive (DNp73) isoforms, with opposite effects on the expression of p53 target genes and on apoptosis induction. The imbalance between DNp73 and TAp73 may contribute to tumorig

  13. The Sensors Connectivity within SCADA Automation Environment and New Trends for Security Development during Multicasting Routing Transmission

    OpenAIRE

    Aamir Shahzad; Kalum Priyanath Udagepola; Young-keun Lee; Soojin Park; Malrey Lee

    2015-01-01

    This study examined the security of SCADA system and its protocols, more specifically, SCADA/DNP3 protocol security. To achieve the study goals, a SCADA simulation environment is designed for water pumping process through connectivity of intelligent sensors, the payload is constructed, and security is deployed inside DNP3 protocol stack and then bytes are multicast to subcontrollers.

  14. A Comparison of the Democratic Security Policy in Colombia and Provincial Reconstruction Teams in Iraq

    Science.gov (United States)

    2009-09-01

    program. Republica de Colombia. Departamento Nacional de Planeacion , Porgrama de Desarrollo Alternativo, Documento CONPES 2734-DNP-UDA-UJS, Bogotá...Ministry of Defense, 2003. Republica de Colombia. “Departmento Nacional de Planeacion , Porgrama de Desarrollo Alternativo, Documento CONPES 2734-DNP

  15. Kerosene/Water/2,4-Dinitrophenol.

    Science.gov (United States)

    Whyman, Derek

    1986-01-01

    Background information/theory, procedures used, and typical results obtained are discussed for a system in which kerosene, water, and 2,4-dinitrophenol (DNP) are used to demonstrate the principles of liquid-liquid extraction in a Graesser rotary contractor. The DNP is almost equal between the two disimilar solvents. (JN)

  16. Barriers to integrating information technology content in doctor of nursing practice curricula.

    Science.gov (United States)

    Lilly, Kezia; Fitzpatrick, Joyce; Madigan, Elizabeth

    2015-01-01

    To date, there is no benchmark data available on the measurement of program outcomes in doctor of nursing practice (DNP) programs. The purpose of this study was to identify barriers to integration of IT content in the curriculum in DNP programs, perceived IT competencies taught, and DNP faculty perception of competencies. The study location was DNP programs in the United States, and focus was on doctorate-prepared faculty with a DNP or PhD. A descriptive design using an Internet-based survey was done with 113 DNP programs administrators and faculty across the United States. Limitation of the study was that few DNP administrators forwarded the study to faculty, limiting the sample size. For the purpose of this study, the results were limited to responses from DNP administrators, and some comparative data of the faculty were used. Barriers measured included lack of qualified faculty, faculty's limited knowledge or skills in IT, lack of interest, age, lack of time to learn IT, lack of time to use IT, too many work demands, lack of administrative vision, unclear expectations of faculty, lack of technical support to faculty, or lack of resources. Leading barriers to IT implementation were lack of time of faculty, too many other work demands of faculty, lack of resources dedicated to IT, and lack of qualified faculty to teach IT. Further research is necessary on doctorate-prepared faculty and on interventions to overcome these barriers is needed. Published by Elsevier Inc.

  17. MEASUREMENT OF AFFINITY IN SERUM SAMPLES OF ANTIGEN-FREE, GERM-FREE AND CONVENTIONAL MICE AFTER HYPERIMMUNIZATION WITH 2,4-DINITROPHENYL KEYHOLE LIMPET HEMOCYANIN, USING SURFACE-PLASMON RESONANCE

    NARCIS (Netherlands)

    BAKKER, R; LASONDER, E; BOS, NA

    We previously investigated the primary and secondary responses and hyperimmunization to the T cell-dependent antigen 2,4-dinitrophenyl keyhole limpet hemocyanin (DNP-KLH) in antigen-free (AF), germ-free (GF) and conventional (CV) mice. Both the absolute and relative numbers of DNP-specific

  18. HUMORAL IMMUNE-RESPONSE TO 2,4-DINITROPHENYL KEYHOLE LIMPET HEMOCYANIN IN ANTIGEN-FREE, GERM-FREE AND CONVENTIONAL BALB/C MICE

    NARCIS (Netherlands)

    BOS, NA; PLOPLIS, VA

    The B cell immune response to 2,4-dinitrophenyl (DNP) keyhole limpet hemocyanin was compared in antigen-free, germ-free and conventional BALB/c mice. The numbers of total and of DNP-specific IgM-, IgG- and IgA-secreting cells in the spleen were determined by enzyme-linked immunosorbent plaque assays

  19. Novel Insights into p63 Expression and Function in Prostate

    Science.gov (United States)

    2007-11-02

    Ad-C control adenovirus AdDNp63 DNp63 adenovirus Admtp53 mtp53 adenovirus ADULT acro- dermato -ungual-lacrimal-tooth Adwtp53 wtp53 adenovirus AR...Mammary (LM) and Acro- Dermato -Ungual-Lacrimal-Tooth (ADULT), with similar clinical features which have also been associated with p63 mutation (Celli

  20. A 282 GHz Probe for Dynamic Nuclear Polarization

    DEFF Research Database (Denmark)

    Rybalko, Oleksandr; Bowen, Sean; Zhurbenko, Vitaliy

    Introduction In DNP, microwave irradiation of a sample facilitates the transfer of spin polarization from electrons tonuclei. One of the way to improve the DNP enhancement is to transfer microwave power from the mm-wave source tothe sample more effectively. Several methods and techniques to effic...

  1. Dissolution Dynamic Nuclear Polarization of Non-Self-Glassing Agents: Spectroscopy and Relaxation of Hyperpolarized [1-13C]Acetate

    DEFF Research Database (Denmark)

    Flori, Alessandra; Liserani, Matteo; Bowen, Sean

    2015-01-01

    The intrinsic physicochemical properties of the sample formulation are the key factors for efficient hyperpolarization through dissolution dynamic nuclear polarization (dissolution-DNP). We provide a comprehensive characterization of the DNP process for Na-[1-13C]acetate selected as a model for non...

  2. Instrumentation and control system architecture of ECRH SST1

    Science.gov (United States)

    Patel, Harshida; Patel, Jatin; purohit, Dharmesh; Shukla, B. K.; Babu, Rajan; Mistry, Hardik

    2017-07-01

    The Electron Cyclotron Resonance Heating (ECRH) system is an important heating system for the reliable start-up of tokamak. The 42GHz and 82.6GHz Gyrotron based ECRH systems are used in tokomaks SST-1 and Aditya to carry out ECRH related experiments. The Gyrotrons are high power microwave tubes used as a source for ECRH systems. The Gyrotrons need to be handled with optimum care right from the installation to its Full parameter control operation. The Gyrotrons are associated with the subsystems like: High voltage power supplies (Beam voltage and anode voltage), dedicated crowbar system, magnet, filament and ion pump power supplies and cooling system. The other subsystems are transmission line, launcher and dummy load. A dedicated VME based data acquisition & control (DAC) system is developed to operate and control the Gyrotron and its associated sub system. For the safe operation of Gyrotron, two level interlocks with fail-safe logic are developed. Slow signals that are operated in scale of millisecond range are programmed through software and hardware interlock in scale of microsecond range are designed and developed indigenously. Water-cooling and the associated interlock are monitored and control by data logger with independent human machine interface.

  3. ECH Technology Development

    Energy Technology Data Exchange (ETDEWEB)

    Temkin, Richard [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

    2014-12-24

    Electron Cyclotron Heating (ECH) is needed for plasma heating, current drive, plasma stability control, and other applications in fusion energy sciences research. The program of fusion energy sciences supported by U. S. DOE, Office of Science, Fusion Energy Sciences relies on the development of ECH technology to meet the needs of several plasma devices working at the frontier of fusion energy sciences research. The largest operating ECH system in the world is at DIII-D, consisting of six 1 MW, 110 GHz gyrotrons capable of ten second pulsed operation, plus two newer gyrotrons. The ECH Technology Development research program investigated the options for upgrading the DIII-D 110 GHz ECH system. Options included extending present-day 1 MW technology to 1.3 – 1.5 MW power levels or developing an entirely new approach to achieve up to 2 MW of power per gyrotron. The research consisted of theoretical research and designs conducted by Communication and Power Industries of Palo Alto, CA working with MIT. Results of the study would be validated in a later phase by research on short pulse length gyrotrons at MIT and long pulse / cw gyrotrons in industry. This research follows a highly successful program of development that has led to the highly reliable, six megawatt ECH system at the DIII-D tokamak. Eventually, gyrotrons at the 1.5 megawatt to multi-megawatt power level will be needed for heating and current drive in large scale plasmas including ITER and DEMO.

  4. The spin-temperature theory of dynamic nuclear polarization and nuclear spin-lattice relaxation

    Science.gov (United States)

    Byvik, C. E.; Wollan, D. S.

    1974-01-01

    A detailed derivation of the equations governing dynamic nuclear polarization (DNP) and nuclear spin lattice relaxation by use of the spin temperature theory has been carried to second order in a perturbation expansion of the density matrix. Nuclear spin diffusion in the rapid diffusion limit and the effects of the coupling of the electron dipole-dipole reservoir (EDDR) with the nuclear spins are incorporated. The complete expression for the dynamic nuclear polarization has been derived and then examined in detail for the limit of well resolved solid effect transitions. Exactly at the solid effect transition peaks, the conventional solid-effect DNP results are obtained, but with EDDR effects on the nuclear relaxation and DNP leakage factor included. Explicit EDDR contributions to DNP are discussed, and a new DNP effect is predicted.

  5. The magnetic field dependence of cross-effect dynamic nuclear polarization under magic angle spinning

    Energy Technology Data Exchange (ETDEWEB)

    Mance, Deni; Baldus, Marc, E-mail: m.baldus@uu.nl [NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, 3584 CH Utrecht (Netherlands); Gast, Peter; Huber, Martina [Department of Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, PO Box 9504, 2300 RA Leiden (Netherlands); Ivanov, Konstantin L., E-mail: ivanov@tomo.nsc.ru [International Tomography Center, Siberian Branch of the Russian Academy of Science, Institutskaya 3a, Novosibirsk 630090, Russia and Novosibirsk State University, Pirogova 2, Novosibirsk 63009 (Russian Federation)

    2015-06-21

    We develop a theoretical description of Dynamic Nuclear Polarization (DNP) in solids under Magic Angle Spinning (MAS) to describe the magnetic field dependence of the DNP effect. The treatment is based on an efficient scheme for numerical solution of the Liouville-von Neumann equation, which explicitly takes into account the variation of magnetic interactions during the sample spinning. The dependence of the cross-effect MAS-DNP on various parameters, such as the hyperfine interaction, electron-electron dipolar interaction, microwave field strength, and electron spin relaxation rates, is analyzed. Electron spin relaxation rates are determined by electron paramagnetic resonance measurements, and calculations are compared to experimental data. Our results suggest that the observed nuclear magnetic resonance signal enhancements provided by MAS-DNP can be explained by discriminating between “bulk” and “core” nuclei and by taking into account the slow DNP build-up rate for the bulk nuclei.

  6. Bis-gadolinium complexes for solid effect and cross effect dynamic nuclear polarization

    Energy Technology Data Exchange (ETDEWEB)

    Kaushik, Monu; Corzilius, Bjoern [Goethe-Universitaet Frankfurt am Main, Institut fuer Physikalische und Theoretische Chemie, Institut fuer Biophysikalische Chemie und Biomolekulares Magnetresonanzzentrum (BMRZ) (Germany); Qi, Mian; Godt, Adelheid [Fakultaet fuer Chemie und Centrum fuer Molekulare Materialien (CM2), Universitaet Bielefeld (Germany)

    2017-04-03

    High-spin complexes act as polarizing agents (PAs) for dynamic nuclear polarization (DNP) in solid-state NMR spectroscopy and feature promising aspects towards biomolecular DNP. We present a study on bis(Gd-chelate)s which enable cross effect (CE) DNP owing to spatial confinement of two dipolar-coupled electron spins. Their well-defined Gd..Gd distances in the range of 1.2-3.4 nm allowed us to elucidate the Gd..Gd distance dependence of the DNP mechanism and NMR signal enhancement. We found that Gd..Gd distances above 2.1 nm result in solid effect DNP while distances between 1.2 and 2.1 nm enable CE for {sup 1}H, {sup 13}C, and {sup 15}N nuclear spins. We compare 263 GHz electron paramagnetic resonance (EPR) spectra with the obtained DNP field profiles and discuss possible CE matching conditions within the high-spin system and the influence of dipolar broadening of the EPR signal. Our findings foster the understanding of the CE mechanism and the design of high-spin PAs for specific applications of DNP. (copyright 2017 Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim)

  7. Utilizing Team Debate to Increase Student Abilities for Mentoring and Critical Appraisal of Global Health Care in Doctor of Nursing Practice Programs.

    Science.gov (United States)

    Elliott, Naomi; Farnum, Karen; Beauchesne, Michelle

    2016-01-01

    Although graduates of doctor of nursing practice (DNP) programs are expected to demonstrate competence in advanced clinical scholarship, mentoring, and leadership, little is published about how team debate on a global health care topic supports DNP student learning and skill development. This article reports on an illuminative evaluation of DNP student learning experiences of team debate in the context of a 2-week international school program in Ireland. A focused illuminative evaluation approach involving a cohort of seven DNP students, who had participated in an international school team debate, was used. Data were collected using a Web-based qualitative questionnaire designed to elicit in-depth reflective accounts of DNP students' learning experiences. Content analysis revealed that team debate on a global health care topic enhanced learning in relation to fostering critical thinking and critical appraisal skills; encouraging teamwork; providing opportunities for mentoring, relationship building, and socialization into profession; and, from the DNP student perspective, increasing knowledge and global understanding of health care. This evaluation provides insights for nurse educators into the benefits of introducing team debate as a group activity to enhancing scholarly inquiry and mentoring skills of DNP students. Further research to evaluate team debate in other nurse education programs is needed. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. Regulatory functions of hapten-reactive helper and suppressor T lymphocytes. I. Detection and characterization of hapten-reactive suppressor T-cell activity in mice immunized with hapten-isologous protein conjugate

    Science.gov (United States)

    Yamamoto, H; Hamaoka, T; Yoshizawa, M; Kuroki, M; Kitagawa, M

    1977-01-01

    Helper and suppressor T-cell activities were detected simultaneously in the spleen cells of mice immunized with para-azobenzoate (PAB)-mouse gammaglobulin (MGG). Dinitrophenyl (DNP)-specific B cells were raised by immunization with DNP-keyhole limpet hemocyanin (KLH) and used as the indicator B-cell population. The helper and suppressor T-cell activities were determined after adoptively transferring spleen cells from PAB-MGG- primed donors and DNP-KLH-primed donors into X-irradiated recipients. Stimulation of these recipients with DNP-MGG-PAB detected helper T-cell activity, which was measured in terms of increased anti-DNP antibody responses of DNP-KLH-primed cells over these responses in the presence of unprimed cells. On the other hand, when DNP-KLH-primed cells were stimulated with DNP-KLH-PAB in the presence of PAB-MGG-primed cells, anti-DNP antibody responses were substantially lower than in unprimed normal cells. This suppressor cell population was (a) hapten-reactive, (b) present in B-cell-depleted spleen cells, (c) Thy-1 positive, (d) detectable earlier than the helper T-cell activities after priming (e) more radiosensitive than helper cells, and (f) found in the spleen but not the lymph nodes in contrast to helper T cells. These data indicate that these suppressor T cells are distinct from the helper T cells. PAB-reactive T cells clearly suppressed the antibody response by inhibiting KLH-reactive helper T-cell functions. The hapten-reactive T-lymphocyte system described here should be useful for analyzing and manipulating the immune response and for studying regulatory interactions of helper and suppressor T cells in the induction of antibody responses. PMID:68994

  9. Combined administration of cerebrolysin and donepezil induces plastic changes in prefrontal cortex in aged mice.

    Science.gov (United States)

    Alcántara-González, Faviola; Mendoza-Perez, Claudia Rebeca; Zaragoza, Néstor; Juarez, Ismael; Arroyo-García, Luis Enrique; Gamboa, Citlalli; De La Cruz, Fidel; Zamudio, Sergio; Garcia-Dolores, Fernando; Flores, Gonzalo

    2012-11-01

    Cerebrolysin (Cbl) shows neurotrophic and neuroprotective properties while donepezil (Dnp) is a potent acetylcholinesterase (AChE) inhibitor, both drugs are prescribed for Alzheimer's disease (AD) treatment. Previous studies have shown that the Dnp and Cbl administered separately, modify dendritic morphology of neurons in the prefrontal cortex and hippocampus in senile rodents. Since the deficit of neurotrophic factor activity is implicated in the degeneration of cholinergic neurons of basal forebrain, a combination therapy of Dnp and Cbl has been tested recently in Alzheimer's patients. However, the plastic changes that may underlie this combined treatment have not yet been explored. We present here the effect of the combined administration of Cbl and Dnp on dendritic morphology in brain regions related to learning and memory in aged mice. The Golgi-Cox staining protocol and Sholl analysis were used for studying dendritic changes. Cbl and Dnp were administrated daily for 2 months to 9-months-old mice. Locomotor activity was assessed, as well as the dendritic morphology of neurons in several limbic regions was analyzed. Results showed that Cbl and Dnp induced an increase in locomotor activity without synergistic effect. The Cbl or Dnp treatment modified the dendritic morphology of neurons from prefrontal cortex (PFC), dorsal hippocampus (DH), dentate gyrus (DG), and the shell of nucleus accumbens (NAcc). These changes show an increase in the total dendritic length and spine density, resulting in an improvement of dendritic arborization. Prominently, a synergistic effect of Cbl and Dnp was observed on branching order and total dendritic length of pyramidal neurons from PFC. These results suggest that Dnp and Cbl may induce plastic changes in a manner independent of each other, but could enhance their effect in target cells from PFC.

  10. 含N,N-二硝基哌嗪无烟改性双基推进剂的燃烧性能%Combustion Characteristics of Smokeless CMDB Propellants Containing N, N' -dinitropiperazine

    Institute of Scientific and Technical Information of China (English)

    刘芳莉; 李吉祯; 齐晓飞; 宋振伟

    2012-01-01

    以CMDB推进剂为基础,用N,N-二硝基哌嗪(DNP)替代推进剂中的RDX,研究了DNP含量、燃烧稳定剂(CaCO3、TiO2、MgO及Al2O3)、燃烧催化剂(铅盐、铅盐/铜盐、铅盐/铜盐/炭黑)对DNP-CMDB推进剂燃烧性能的影响.结果表明,DNP可明显降低无烟CMDB推进剂的燃速,当DNP完全替代RDX时,在18MPa压强下推进剂的燃速降低约68%;铅盐/铜盐/炭黑燃烧催化剂复配体系能够有效降低DNP-CMDB推进剂的燃速压强指数,使其出现平台燃烧效应.%N,N'- dinitropiperazine(DNP)was used to replace RDX in RDX-composite modified double base(CMDB) propellant,and the influences of DNP content,combustion stabilizers (CaCO3 ,TiO2 ,MgO and Al2O3 ) and combustion catalysts (Pb salt,Pb salt/Cu salt/Carbon black) on the combustion characteristics of DNP-CMDB propellant were studied. Results showed that the burning rate of CMDB propellants containing DNP could be decreased obviously. The burning rate of CMDB propellant at the pressure of 18. 0 MPa was reduced by 68% when RDX in the propellant was completely replaced by DNP. The pressure exponent of the DNP-CMDB propellants could be reduced obviously by the addition of Pb salt/Cu salt/Carbon black catalysts and there were plateaus in some pressure ranges for the combustion of the propellant.

  11. Competition of chemically related antigens for presentation by accessory cells to T cells requires expenditure of metabolic energy by the accessory cells

    DEFF Research Database (Denmark)

    Werdelin, O; Buus, S

    1983-01-01

    The immune responsiveness of guinea pigs both to dinitrophenyl-poly-L-lysine (DNP-PLL) and to the lysine-rich random copolymer of L-glutamic acid and L-lysine (GL) is controlled by the 'poly-L-lysine gene'. We have previously demonstrated that accessory cells of responder strains can be made...... incapable of presenting DNP-PLL to responsive T cells in assays for proliferation, by in vitro exposure of the cells to GL before and during their exposure to DNP-PLL. We demonstrate here that the presence of anti-Ia antibody in the cultures does not interfere with the apparent competition of the two...

  12. Frozen Acrylamide Gels as Dynamic Nuclear Polarization Matrices.

    KAUST Repository

    Viger-Gravel, Jasmine

    2017-05-24

    We show that aqueous acrylamide gels can be used to provide dynamic nuclear polarization (DNP) NMR signal enhancements of around 200 at 9.4 T and 100 K. The enhancements are shown to increase with cross linker concentration and low concentrations of the AMUPol biradical. We show that this DNP matrix can be used in situations where conventional incipient wetness methods fail, such as to obtain DNP surface enhanced NMR spectra from inorganic nanoparticles. In particular, we obtain 113Cd spectra from CdTe-COOH NPs in minutes. The spectra clearly indicate a highly-disordered cadmium rich surface.

  13. Identification of oxidised proteins in the matrix of rice leaf mitochondria by immunoprecipitation and two-dimensional liquid chromatography-tandem mass spectrometry

    DEFF Research Database (Denmark)

    Kristensen, B.K.; Askerlund, P.; Bykova, N.V.

    2004-01-01

    Highly purified mitochondria were isolated from green 7-day-old rice leaves. The mitochondria were sonicated and the matrix fraction isolated as the 100,000g supernatant. Part of the matrix fraction was left untreated while the other part was subjected to a mild oxidative treatment (0.5 mM H2O2 + 0.......2 mM CuSO4 for 10 min at room temperature). The oxidised proteins in both samples were tagged with dinitrophenylhydrazine (DNP), which forms a covalent bond with carbonyl groups. The DNP-tagged proteins were immunoprecipitated using anti-DNP antibodies and digested with trypsin. The mixture...

  14. The ethics curriculum for doctor of nursing practice programs.

    Science.gov (United States)

    Peirce, Anne Griswold; Smith, Jennifer A

    2008-01-01

    Ethical questions dealt with by nurses who have Doctor of Nursing Practice (DNP) degrees include traditional bioethical questions, but also business and legal ethics. Doctorally prepared nurses are increasingly in positions to make ethical decisions rather than to respond to decisions made by others. The traditional master's-degree advanced practice nursing curriculum does not address the extended expertise and decision-making skills needed by DNP practitioners as they face these new types of ethical dilemmas. We propose that a curricular framework that addresses clinical, research, business, and legal ethics is needed by all DNP students.

  15. Dynamic nuclear polarization and nuclear magnetic resonance in the simplest pseudospin quantum Hall ferromagnet

    Science.gov (United States)

    Liu, H. W.; Yang, K. F.; Mishima, T. D.; Santos, M. B.; Hirayama, Y.

    2010-12-01

    We present dynamic nuclear polarization (DNP) in the simplest pseudospin quantum Hall ferromagnet (QHF) of an InSb two-dimensional electron gas with a large g factor using tilted magnetic fields. The DNP-induced amplitude change in a resistance spike of the QHF at large current enables observation of the resistively detected nuclear magnetic resonance of the high nuclear spin isotope I115n with nine quadrupole splittings. Our results demonstrate the importance of domain structures in the DNP process. The nuclear spin relaxation time T1 in this QHF was relatively short (˜120s) and almost temperature independent.

  16. High-Fidelity Bidirectional Nuclear Qubit Initialization in SiC

    Science.gov (United States)

    Ivády, Viktor; Klimov, Paul V.; Miao, Kevin C.; Falk, Abram L.; Christle, David J.; Szász, Krisztián; Abrikosov, Igor A.; Awschalom, David D.; Gali, Adam

    2016-11-01

    Dynamic nuclear polarization (DNP) is an attractive method for initializing nuclear spins that are strongly coupled to optically active electron spins because it functions at room temperature and does not require strong magnetic fields. In this Letter, we theoretically demonstrate that DNP, with near-unity polarization efficiency, can be generally realized in weakly coupled electron spin-nuclear spin systems. Furthermore, we theoretically and experimentally show that the nuclear spin polarization can be reversed by magnetic field variations as small as 0.8 Gauss. This mechanism offers new avenues for DNP-based sensors and radio-frequency free control of nuclear qubits.

  17. Determination of electromagnetic modes in oversized corrugated waveguides on the electron cyclotron resonance heating installation at the tokamak Tore Supra; Determination de modes electromagnetiques de guides d'ondes corrugues surdimensionnes sur l'installation de chauffage des electrons de tokamak Tore Supra

    Energy Technology Data Exchange (ETDEWEB)

    Courtois, L

    2001-03-09

    Electron cyclotron resonance heating (ECRH) in the Tore Supra tokamak constitutes an important step in the research aimed at obtaining thermonuclear fusion reactions. Electron heating is achieved by transmitting an electromagnetic wave from the oscillators (gyrotrons) to the plasma via the fundamental mode, propagating in oversized corrugated waveguides. Maximizing the proportion of the gyrotron power coupled to the fundamental waveguide mode is essential for the good functioning of the transmission line and for maximizing the effect on the plasma. This thesis gives all necessary tools for finding the proportion of the fundamental mode and all other modes present in passive components and at the output of the gyrotron as installed in the Tore Supra ECRH plant. This characterisation is based on obtaining amplitude and phase diagrams of the electric field on a plane transverse to the propagation axis. The most difficult part of obtaining these diagrams is measuring the phase which, despite the very short wavelength, is measured directly at low power levels. At high power levels the phase is numerically reconstructed from amplitude measurements for gyrotron characterisation. A complete theoretical study of the phase reconstruction code is given including its validation with theoretical diagrams. This study allows the realisation of a modal characterisation unit electromagnetic for measurement of radiated beams and usable in each part of the ECRH installation. At the end, the complete modal characterisation is given at low level for a mode converter and also at high level for the first series gyrotron installed at TORE SUPRA. (author)

  18. Design of the ITER Electron Cyclotron Heating and Current Drive Waveguide Transmission Line

    Science.gov (United States)

    Bigelow, T. S.; Rasmussen, D. A.; Shapiro, M. A.; Sirigiri, J. R.; Temkin, R. J.; Grunloh, H.; Koliner, J.

    2007-11-01

    The ITER ECH transmission line system is designed to deliver the power, from twenty-four 1 MW 170 GHz gyrotrons and three 1 MW 127.5 GHz gyrotrons, to the equatorial and upper launchers. The performance requirements, initial design of components and layout between the gyrotrons and the launchers is underway. Similar 63.5 mm ID corrugated waveguide systems have been built and installed on several fusion experiments; however, none have operated at the high frequency and long-pulse required for ITER. Prototype components are being tested at low power to estimate ohmic and mode conversion losses. In order to develop and qualify the ITER components prior to procurement of the full set of 24 transmission lines, a 170 GHz high power test of a complete prototype transmission line is planned. Testing of the transmission line at 1-2 MW can be performed with a modest power (˜0.5 MW) tube with a low loss (10-20%) resonant ring configuration. A 140 GHz long pulse, 400 kW gyrotron will be used in the initial tests and a 170 GHz gyrotron will be used when it becomes available. Oak Ridge National Laboratory, managed by UT-Battelle, LLC, for the U.S. Dept. of Energy under contract DE-AC05-00OR22725.

  19. Initial results for a 170 GHz high power ITER waveguide component test stand

    Science.gov (United States)

    Bigelow, Timothy; Barker, Alan; Dukes, Carl; Killough, Stephen; Kaufman, Michael; White, John; Bell, Gary; Hanson, Greg; Rasmussen, Dave

    2014-10-01

    A high power microwave test stand is being setup at ORNL to enable prototype testing of 170 GHz cw waveguide components being developed for the ITER ECH system. The ITER ECH system will utilize 63.5 mm diameter evacuated corrugated waveguide and will have 24 >150 m long runs. A 170 GHz 1 MW class gyrotron is being developed by Communications and Power Industries and is nearing completion. A HVDC power supply, water-cooling and control system has been partially tested in preparation for arrival of the gyrotron. The power supply and water-cooling system are being designed to operate for >3600 second pulses to simulate the operating conditions planned for the ITER ECH system. The gyrotron Gaussian beam output has a single mirror for focusing into a 63.5 mm corrugated waveguide in the vertical plane. The output beam and mirror are enclosed in an evacuated duct with absorber for stray radiation. Beam alignment with the waveguide is a critical task so a combination of mirror tilt adjustments and a bellows for offsets will be provided. Analysis of thermal patterns on thin witness plates will provide gyrotron mode purity and waveguide coupling efficiency data. Pre-prototype waveguide components and two dummy loads are available for initial operational testing of the gyrotron. ORNL is managed by UT-Battelle, LLC, for the U.S. Dept. of Energy under Contract DE-AC-05-00OR22725.

  20. Compact wavelength router based on a Silicon-on-insulator arrayed waveguide grating pigtailed to a fiber array.

    Science.gov (United States)

    Dumon, P; Bogaerts, W; Van Thourhout, D; Taillaert, D; Baets, R; Wouters, J; Beckx, S; Jaenen, P

    2006-01-23

    We demonstrate a compact, fiber-pigtailed, 4-by-4 wavelength router in Silicon-on-insulator photonic wires, fabricated using CMOS processing methods. The core is an AWG with a 250GHz channel spacing and 1THz free spectral range, on a 425x155 microm(2) footprint. The insertion loss of the AWG was reduced to 3.5dB by applying a two-step processing technique. The crosstalk is -12dB. The device was pigtailed using vertical fiber couplers and an eight-fiber array connector.

  1. Photoassociative production of ultracold heteronuclear YbLi* molecules

    Science.gov (United States)

    Roy, Richard; Shrestha, Rajendra; Green, Alaina; Gupta, Subhadeep; Li, Ming; Kotochigova, Svetlana; Petrov, Alexander; Yuen, Chi Hong

    2016-09-01

    We report on the production of ultracold heteronuclear YbLi* molecules in a dual-species magneto-optical trap by photoassociation (PA). The formation of the electronically excited molecules close to dissociation was observed by trap loss spectroscopy. We find 4 rovibrational states within a range of 250 GHz below the Yb (S10) +Li (1/2 2P) asymptote and observe isotopic PA line shifts in mixtures of 6Li with 174Yb, 172Yb, and 176Yb. We also describe our theoretical ab initio calculation for the relevant electronic potentials and utilize it to analyze and identify the lines in the experimentally observed spectrum.

  2. Photoassociative production of ultracold heteronuclear YbLi* molecules

    CERN Document Server

    Roy, Richard; Green, Alaina; Gupta, Subhadeep; Li, Ming; Kotochigova, Svetlana; Petrov, Alexander; Yuen, Chi Hong

    2016-01-01

    We report on the production of ultracold heteronuclear YbLi* molecules in a dual-species magneto-optical trap by photoassociation (PA). The formation of the electronically excited molecules close to dissociation was observed by trap loss spectroscopy. We find 4 rovibrational states within a range of $250\\,$GHz below the Yb($^1S_0$) + Li($^2P_{1/2}$) asymptote and observe isotopic PA line shifts in mixtures of $^6$Li with $^{174}$Yb, $^{172}$Yb, and $^{176}$Yb. We also describe our theoretical ab-initio calculation for the relevant electronic potentials and utilize it to analyze and identify the lines in the experimentally observed spectrum.

  3. Observation of ferromagnetic resonance in strontium ruthenate (SrRuO3)

    Energy Technology Data Exchange (ETDEWEB)

    Langner, Matthew C.; Kantner, Colleen L.S.; Chu, Y.H.; Martin, Lane M.; Yu, Pu; Ramesh, R.; Orenstein, Joe

    2008-12-03

    We report the observation of ferromagnetic resonance (FMR) in SrRuO3 using the time-resolved magnetooptical Kerr effect. The FMR oscillations in the time-domain appear in response to a sudden, optically induced change in the direction of easy-axis anistropy. The high FMR frequency, 250 GHz, and large Gilbert damping parameter, alpha ~;; 1, are consistent with strong spin-orbit coupling. We find that the parameters associated with the magnetization dynamics, including alpha, have a non-monotonic temperature dependence, suggestive of a link to the anomalous Hall effect.

  4. Dynamic nuclear polarization for magnetic resonance imaging. An in-bore approach

    Energy Technology Data Exchange (ETDEWEB)

    Krummenacker, Jan G.

    2012-07-01

    In this thesis, the development of an in-bore liquid state DNP polarizer for MRI applications operating in flow through mode at a magnetic field strength of 1.5 T was described. After an introductory chapter 1 and a chapter 2 on the theoretical background, chapter 3 dealt chiefly with the challenge of performing liquid state DNP at a high magnetic field of 9.2 T. The feasibility of performing liquid state DNP at this field was demonstrated for various solvents, as well as for metabolites in solution. Chapter 4 then moved to the aim of this work, the application of liquid state DNP for MRI experiments. It introduced the rationale of our approach, the hardware that was developed and demonstrated its performance in a clinical MRI tomograph.

  5. Smoking Plus Diabetes a Very Deadly Mix

    Science.gov (United States)

    ... Patricia Folan, D.N.P., director, Center for Tobacco Control, Northwell Health, Great Neck, N.Y.; Joel Zonszein, M.D., director, Clinical Diabetes Center, Montefiore Medical Center, New York City; Gerald ...

  6. Resonance-inclined optical nuclear spin polarization of liquids in diamond structures

    CERN Document Server

    Chen, Qiong; Jelezko, Fedor; Retzker, Alex; Plenio, Martin B

    2015-01-01

    Dynamic nuclear polarization (DNP) of molecules in a solution at room temperature has potential to revolutionize nuclear magnetic resonance spectroscopy and imaging. The prevalent methods for achieving DNP in solutions are typically most effective in the regime of small interaction correlation times between the electron and nuclear spins, limiting the size of accessible molecules. To solve this limitation, we design a mechanism for DNP in the liquid phase that is applicable for large interaction correlation times. Importantly, while this mechanism makes use of a resonance condition similar to solid-state DNP, the polarization transfer is robust to a relatively large detuning from the resonance due to molecular motion. We combine this scheme with optically polarized nitrogen vacancy (NV) center spins in nanodiamonds to design a setup that employs optical pumping and is therefore not limited by room temperature electron thermal polarisation. We illustrate numerically the effectiveness of the model in a flow cel...

  7. Recommendations for Creating a Resource Management System to Support the Colombian Army in a New Environment

    Science.gov (United States)

    1992-04-15

    World Military and Social Expenditures. 1991. 4. Republica de Colombia, Departamento Nacional de Planeacion , Documento DNP-2570-UIP-MinHacienda...Center, The Planning. Programmina and Budgeting System (PPBS), Technical Report, 1991. Departamento Nacional de Planeacion de Colombia, Plan ouinuenal

  8. Doctor of Nursing Practice programs: opportunities for faculty development.

    Science.gov (United States)

    Sebastian, Juliann G; White Delaney, Connie

    2013-08-01

    This article examines development opportunities for faculty teaching in Doctor of Nursing Practice (DNP) programs. Although faculty development for DNP programs is similar to that of other academic programs, faculty may need different strategies for teaching, scholarship, and service because DNP programs focus on translation of science into practice, systems-level changes, clinical scholarship, and the highest levels of advanced nursing practice. Faculty and student collaboration across DNP and PhD programs provide new approaches for translating research into practice and generating practice questions in need of further scientific development. Specific faculty development strategies for facilitating this collaboration are essential. Capstone projects pose special opportunities for faculty development due to the integration of these projects within diverse practice environments, with differing expectations, regulations, and pacing compared with research. Linking new care delivery models with health informatics is expected to facilitate rapid translation of research and development of improvements in practice. Copyright 2013, SLACK Incorporated.

  9. Synthesis of long T silicon nanoparticles for hyperpolarized Si magnetic resonance imaging

    DEFF Research Database (Denmark)

    Atkins, T.M.; Ganguly, S.; Kauzlarich, S.M.

    2013-01-01

    We describe the synthesis, materials characterization, and dynamic nuclear polarization (DNP) of amorphous and crystalline silicon nanoparticles for use as hyperpolarized magnetic resonance imaging (MRI) agents. The particles were synthesized by means of a metathesis reaction between sodium silic...

  10. Manipulation of dynamic nuclear spin polarization in single quantum dots by photonic environment engineering

    Science.gov (United States)

    Fong, C. F.; Ota, Y.; Iwamoto, S.; Arakawa, Y.

    2017-06-01

    Optically induced dynamic nuclear spin polarization (DNP) in a semiconductor quantum dot (QD) requires many cycles of excitation of spin polarized carriers and carrier recombination. As such, the radiative lifetime of the exciton containing the electron becomes one of the limiting factors of DNP. In principle, changing the radiative lifetime of the exciton will affect DNP and thus the nuclear spin polarization. Here, we demonstrate the manipulation of DNP in single QDs through the engineering of the photonic environment using two-dimensional photonic crystals. We find that the achievable degree of nuclear spin polarization can be controlled through the modification of exciton radiative lifetime. Our results show the promise of achieving a higher degree of nuclear spin polarization via photonic environment engineering, with implications on spin-based quantum information processing.

  11. Azole energetic materials: initial mechanisms for the energy release from electronical excited nitropyrazoles.

    Science.gov (United States)

    Yuan, Bing; Yu, Zijun; Bernstein, Elliot R

    2014-01-21

    Decomposition of energetic material 3,4-dinitropyrazole (DNP) and two model molecules 4-nitropyrazole and 1-nitropyrazole is investigated both theoretically and experimentally. The initial decomposition mechanisms for these three nitropyrazoles are explored with complete active space self-consistent field (CASSCF) level. The NO molecule is observed as an initial decomposition product from all three materials subsequent to UV excitation. Observed NO products are rotationally cold (energetic material DNP, NO is produced on the ground state surface, as the S1 decomposition pathway is energetically unavailable. The theoretically predicted mechanism is consistent with the experimental results, as DNP decomposes in a lower electronic state than do the model systems and thus the vibrational energy in the NO product from DNP should be hotter than from the model systems. The observed rotational energy distributions for NO are consistent with the final structures of the respective transition states for each molecule.

  12. 78 FR 37234 - Notice of Invitation; Coal Exploration License Application NDM 105349, ND

    Science.gov (United States)

    2013-06-20

    ... Office, 5001 Southgate Drive, Billings, Montana. A written notice to participate in the exploration license should be sent to the State Director, BLM Montana State Office, 5001 Southgate Drive, Billings, MT.... BILLING CODE 4310-DN-P...

  13. Topical Developments in High-Field Dynamic Nuclear Polarization

    Science.gov (United States)

    Kiesewetter, Matthew K.; Frantz, Derik K.; Walish, Joseph J.; Ravera, Enrico; Luchinat, Claudio; Swager, Timothy M.; Griffin, Robert G.

    2015-01-01

    We report our recent efforts directed at improving high-field DNP experiments. We investigated a series of thiourea nitroxide radicals and the associated DNP enhancements ranging from ε = 25 to 82 that demonstrate the impact of molecular structure on performance. We directly polarized low-gamma nuclei including 13C, 2H, and 17O using trityl via the cross effect. We discuss a variety of sample preparation techniques for DNP with emphasis on the benefit of methods that do not use a glass-forming cryoprotecting matrix. Lastly, we describe a corrugated waveguide for use in a 700 MHz / 460 GHz DNP system that improves microwave delivery and increases enhancements up to 50%. PMID:25977588

  14. 1H and 15N Dynamic Nuclear Polarization Studies of Carbazole

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Jian Zhi; Solum, Mark S.; Wind, Robert A.; Nilsson, Brad L.; Peterson, Matt A.; Pugmire, Ronald J.; Grant, David M.

    2000-01-01

    15N NMR experiments, combined with dynamic nuclear polarization (DNP), are reported on carbazole doped with the stable free radical 1,3 bisdiphenylene-2 phenylally1 (BDPA). Doping shortens the nuclear relaxation times and provides paramagnetic centers that can be used to enhance the nuclear signal by means of DNP so that 15 N NMR experiments can be done in minutes. The factors were measured in a 1.4 T external field, using both unlabeled and 98% 15N labeled carbazole with doping levels varying between 0.65 and 5.0 wt % BDPA. A doping level of approximately 1 wt % produced optimal results. DNP enhancement factors of 35 and 930 were obtained for 1H and 15N, respectively making it possible to perform 15N DNP NMR experiments at the natural abundance level.

  15. A two-dimensionally focusing, quasi-optical antenna for millimeter-wave scattering in plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Idehara, T.; Tatsukawa, T. (Faculty of Engineering, Fukui University, Fukui 910, Japan (JP)); Brand, G.F.; Fekete, P.W.; Moore, K.J. (School of Physics, University of Sydney, NSW 2006 (Australia))

    1990-06-01

    A two-dimensionally focusing, quasi-optical antenna having one elliptical reflector and one parabolic reflector has been built for use with a tunable gyrotron in order to carry out millimeter-wave scattering measurements on the TORTUS tokamak plasma at the University of Sydney. The advantages of this antenna are the following: (1) The elliptical reflector focuses the radiation beam in the toroidal direction, while the parabolic reflector focuses in the direction of major radius. This gives excellent two-dimensional focusing in the plasma region, and consequently excellent spatial resolution. (2) The focal point can be easily swept along the direction of major radius in the whole plasma region, simply by changing the angle of the parabolic reflector by a small amount. These features have been demonstrated experimentally using the tunable gyrotron source, GYROTRON III, and in computations of the radiated fields.

  16. Remote Control System of the TJ-II Microwave Transmission Lines Mirrors; Sistema de Control Remoto de los Espejos de las Lineas de Transmision de Microondas del TJ-II

    Energy Technology Data Exchange (ETDEWEB)

    Lopez Sanchez, A.; Fernandez, A.; Cappa, A.; Gama, J. de la; Olivares, J.; Garcia, R.; Chamorro, M.

    2007-09-27

    The ECRH system of the TJ-II stellarator has two gyrotrons, which deliver a maximum power of 300 kW each at a frequency of 53.2 GHz. Another 28 GHz gyrotron will be used to heat the plasma by electron Bernstein waves (EBWH). The microwave power is transmitted from the gyrotrons to the vacuum chamber by two quasi-optical transmission lines for ECRH and a corrugated waveguide for EBWH. All transmission lines have an internal movable mirror inside the vacuum chamber to focus the beam and to be able to change the launching angle. The control of the beam polarization is very important and the lines have two corrugated mirrors, which actuate as polarizers. In this report the control system of the position of these three internal mirrors and the polarizers of the EBWH transmission line is described. (Author) 20 refs.

  17. Use of a problem-based learning exercise to teach the lean 8-step problem-solving method.

    Science.gov (United States)

    Tovar, Elizabeth G; Warshawsky, Nora

    2015-01-01

    Doctor of nursing practice (DNP) graduates must be prepared to lead quality improvement (QI) initiatives in health care settings; however, effective and feasible teaching strategies pose a challenge for many DNP program faculties. This article describes a successful and practical problem-based learning exercise for students to work through the QI process using the Lean 8-step problem-solving method. Suggestions for faculty and recommendations for future activities are discussed.

  18. Leadership redefined: educating the Doctorate of Nursing Practice nurse leader through innovation.

    Science.gov (United States)

    Montgomery, Kathryn Lothschuetz

    2011-01-01

    In today's society, health care systems are characterized by change, unpredictability, increasing speed of information and knowledge exchanges, redefined organizational boundaries and hierarchy, emphasis on value, teamwork, interdisciplinary collaboration, diversity, and interconnectedness. This new reality has forced nurse educators to redefine nursing leadership and prepare the Doctorate of Nursing Practice (DNP) leader through innovative courses offering experiential learning based on complex adaptive systems and quantum leadership theory. This article describes the experiential learning approach and integrated learning experience for DNP students.

  19. Multifunctional Materials: Transparent Reactive Armor Utilizing Single-Walled Carbon Nanotube Frameworks

    Science.gov (United States)

    2010-03-03

    reagent was needed for DNP- SWNT than for MNP -SWNT if a similar level of functionalization was desired. However, for TNP-SWNT, a certain level of...It should be noted that MNP -SWNT has the highest level of functionalization and TNP- SWNT has the lowest, which is the reason why all NP-SWNTs...appear to produce similar amount of heat. The reaction completed within the scanning cycle for TNP-SWNT but not for DNP- and MNP -SWNT. In addition

  20. Development of Sampling and Preservation Techniques to Retard Chemical and Biological Changes in Water Samples

    Science.gov (United States)

    1983-06-24

    accomplishing the original scope of work (development of sampling and pres- ervation techniques). System No. I is described in Table 1. Quadruplicate SARM ...Rate: 1.0 ml/min Injection Volume: 40-100 p1 Quadruplicate SARM reference solutions were analyzed for the four munitions at the following concentration...tion of SARM Reference Solutions of DNP, RDX, TNBI DNB, 2,4-DNT, TNT, Tetryl, and DPA ...... .............. A-6 2 Statistical Evaluation of DNP in

  1. Quantitative dynamic nuclear polarization-NMR on blood plasma for assays of drug metabolism.

    Science.gov (United States)

    Lerche, Mathilde H; Meier, Sebastian; Jensen, Pernille R; Hustvedt, Svein-Olaf; Karlsson, Magnus; Duus, Jens Ø; Ardenkjaer-Larsen, Jan H

    2011-01-01

    Analytical platforms for the fast detection, identification and quantification of circulating drugs with a narrow therapeutic range are vital in clinical pharmacology. As a result of low drug concentrations, analytical tools need to provide high sensitivity and specificity. Dynamic nuclear polarization-NMR (DNP-NMR) in the form of the hyperpolarization-dissolution method should afford the sensitivity and spectral resolution for the direct detection and quantification of numerous isotopically labeled circulating drugs and their metabolites in single liquid-state NMR transients. This study explores the capability of quantitative in vitro DNP-NMR to assay drug metabolites in blood plasma. The lower limit of detection for the anti-epileptic drug (13)C-carbamazepine and its pharmacologically active metabolite (13)C-carbamazepine-10,11-epoxide is 0.08 µg/mL in rabbit blood plasma analyzed by single-scan (13)C DNP-NMR. An internal standard is used for the accurate quantification of drug and metabolite. Comparison of quantitative DNP-NMR data with an established analytical method (liquid chromatography-mass spectrometry) yields a Pearson correlation coefficient r of 0.99. Notably, all DNP-NMR determinations were performed without analyte derivatization or sample purification other than plasma protein precipitation. Quantitative DNP-NMR is an emerging methodology which requires little sample preparation and yields quantitative data with high sensitivity for therapeutic drug monitoring.

  2. HIV-Associated Distal Neuropathic Pain is Associated with Smaller Total Cerebral Cortical Gray Matter

    Science.gov (United States)

    Keltner, John R.; Fennema-Notestine, Christine; Vaida, Florin; Wang, Dongzhe; Franklin, Donald R.; Dworkin, Robert H.; Sanders, Chelsea; McCutchan, J. Allen; Archibald, Sarah L.; Miller, David J.; Kesidis, George; Cushman, Clint; Kim, Sung Min; Abramson, Ian; Taylor, Michael J.; Theilmann, Rebecca J.; Julaton, Michelle D.; Notestine, Randy J.; Corkran, Stephanie; Cherner, Mariana; Duarte, Nichole A.; Alexander, Terry; Robinson-Papp, Jessica; Gelman, Benjamin B.; Simpson, David M.; Collier, Ann C.; Marra, Christina M.; Morgello, Susan; Brown, Greg; Grant, Igor; Atkinson, J. Hampton; Jernigan, Terry L.; Ellis, Ronald J.

    2014-01-01

    Despite modern antiretroviral therapy, HIV-associated sensory neuropathy affects over 50% of HIV patients. The clinical expression of HIV neuropathy is highly variable: many individuals report few symptoms, but about half report distal neuropathic pain (DNP), making it one of the most prevalent, disabling and treatment-resistant complications of HIV disease. The presence and intensity of pain is not fully explained by the degree of peripheral nerve damage, making it unclear why some patients do, and others do not, report pain. To better understand central nervous system contributions to HIV DNP, we performed a cross-sectional analysis of structural magnetic resonance imaging (MRI) volumes in 241 HIV-infected participants from an observational multi-site cohort study at five US sites (CNS HIV Antiretroviral Treatment Effects Research Study, CHARTER). The association between DNP and the structural imaging outcomes was investigated using both linear and nonlinear (Gaussian Kernel support vector) multivariable regression, controlling for key demographic and clinical variables. Severity of DNP symptoms was correlated with smaller total cerebral cortical gray matter volume (R = −0.24; p = 0.004). Understanding the mechanisms for this association between smaller total cortical volumes and DNP may provide insight into HIV DNP chronicity and treatment-resistance. PMID:24549970

  3. Molecular dynamics-based selectivity for Fast-Field-Cycling relaxometry by Overhauser and solid effect dynamic nuclear polarization

    Science.gov (United States)

    Neudert, Oliver; Mattea, Carlos; Stapf, Siegfried

    2017-03-01

    In the last decade nuclear spin hyperpolarization methods, especially Dynamic Nuclear Polarization (DNP), have provided unprecedented possibilities for various NMR techniques by increasing the sensitivity by several orders of magnitude. Recently, in-situ DNP-enhanced Fast Field Cycling (FFC) relaxometry was shown to provide appreciable NMR signal enhancements in liquids and viscous systems. In this work, a measurement protocol for DNP-enhanced NMR studies is introduced which enables the selective detection of nuclear spin hyperpolarized by either Overhauser effect or solid effect DNP. Based on field-cycled DNP and relaxation studies it is shown that these methods allow for the independent measurement of polymer and solvent nuclear spins in a concentrated solution of high molecular weight polybutadiene in benzene doped with α,γ-bisdiphenylene-β-phenylallyl radical. Appreciable NMR signal enhancements of about 10-fold were obtained for both constituents. Moreover, qualitative information about the dynamics of the radical and solvent was obtained. Selective DNP-enhanced FFC relaxometry is applied for the measurement of the 1H nuclear magnetic relaxation dispersion of both constituents with improved precision. The introduced method is expected to greatly facilitate NMR studies of complex systems with multiple overlapping signal contributions that cannot be distinguished by standard methods.

  4. HIV-associated distal neuropathic pain is associated with smaller total cerebral cortical gray matter.

    Science.gov (United States)

    Keltner, John R; Fennema-Notestine, Christine; Vaida, Florin; Wang, Dongzhe; Franklin, Donald R; Dworkin, Robert H; Sanders, Chelsea; McCutchan, J Allen; Archibald, Sarah L; Miller, David J; Kesidis, George; Cushman, Clint; Kim, Sung Min; Abramson, Ian; Taylor, Michael J; Theilmann, Rebecca J; Julaton, Michelle D; Notestine, Randy J; Corkran, Stephanie; Cherner, Mariana; Duarte, Nichole A; Alexander, Terry; Robinson-Papp, Jessica; Gelman, Benjamin B; Simpson, David M; Collier, Ann C; Marra, Christina M; Morgello, Susan; Brown, Greg; Grant, Igor; Atkinson, J Hampton; Jernigan, Terry L; Ellis, Ronald J

    2014-06-01

    Despite modern antiretroviral therapy, HIV-associated sensory neuropathy affects over 50 % of HIV patients. The clinical expression of HIV neuropathy is highly variable: many individuals report few symptoms, but about half report distal neuropathic pain (DNP), making it one of the most prevalent, disabling, and treatment-resistant complications of HIV disease. The presence and intensity of pain is not fully explained by the degree of peripheral nerve damage, making it unclear why some patients do, and others do not, report pain. To better understand central nervous system contributions to HIV DNP, we performed a cross-sectional analysis of structural magnetic resonance imaging volumes in 241 HIV-infected participants from an observational multi-site cohort study at five US sites (CNS HIV Anti-Retroviral Treatment Effects Research Study, CHARTER). The association between DNP and the structural imaging outcomes was investigated using both linear and nonlinear (Gaussian Kernel support vector) multivariable regression, controlling for key demographic and clinical variables. Severity of DNP symptoms was correlated with smaller total cerebral cortical gray matter volume (r = -0.24; p = 0.004). Understanding the mechanisms for this association between smaller total cortical volumes and DNP may provide insight into HIV DNP chronicity and treatment-resistance.

  5. Investigation of first mirror heating for the collective Thomson scattering diagnostic in ITER

    DEFF Research Database (Denmark)

    Salewski, Mirko; Meo, Fernando; Bindslev, Henrik

    2008-01-01

    Collective Thomson scattering (CTS) has the capabilities to measure phase space densities of fast ion populations in ITER resolved in configuration space, in velocity space, and in time. In the CTS system proposed for ITER, probing radiation at 60 GHz generated by two 1 MW gyrotrons is scattered...... in the plasma and collected by arrays of receivers. The transmission lines from the gyrotrons to the plasma and from the plasma to the receivers contain several quasioptical mirrors among other components. These are designed to produce astigmatic beam patterns in the plasma where the beam shapes will have...

  6. Radio and millimeter properties of $z \\sim 5.7$ Ly$\\alpha$ emitters in the COSMOS field: limits on radio AGN, submm galaxies, and dust obscuration

    CERN Document Server

    Carilli, C L; Wang, R; Schinnerer, E; Taniguchi, Y; Smolcic, V; Bertoldi, F; Ajiki, M; Nagao, T; Sasaki, S S; Shioya, Y; Aguirre, J E; Blain, A W; Scoville, N Z; Sanders, D B

    2006-01-01

    We present observations at 1.4 and 250 GHz of the $z\\sim 5.7$ Ly$\\alpha$ emitters (LAE) in the COSMOS field found by Murayama et al.. At 1.4 GHz there are 99 LAEs in the lower noise regions of the radio field. We do not detect any individual source down to 3$\\sigma$ limits of $\\sim 30\\mu$Jy beam$^{-1}$ at 1.4 GHz, nor do we detect a source in a stacking analysis, to a 2$\\sigma$ limit of $2.5\\mu$Jy beam$^{-1}$. At 250 GHz we do not detect any of the 10 LAEs that are located within the central regions of the COSMOS field covered by MAMBO ($20' \\times 20'$) to a typical 2$\\sigma$ limit of $S_{250} 6\\times 10^{24}$ W Hz$^{-1}$ in the LAE sample. The radio and millimeter observations also rule out any highly obscured, extreme starbursts in the sample, ie. any galaxies with massive star formation rates $> 1500$ M$_\\odot$ year$^{-1}$ in the full sample (based on the radio data), or 500 M$_\\odot$ year$^{-1}$ for the 10% of the LAE sample that fall in the central MAMBO field. The stacking analysis implies an upper li...

  7. Exploring the high frequency emission of radio loud X-ray binaries

    CERN Document Server

    Paredes, J M; Peracaula, M; Pooley, G G; Mirabel, I F

    2000-01-01

    We report millimetre-wave continuum observations of the X-ray binaries Cygnus X-3, SS 433, LSI+61 303, Cygnus X-1 and GRS 1915+105. The observations were carried out with the IRAM 30 m-antenna at 250 GHz (1.25 mm) from 1998 March 14 to March 20. These millimetre measurements are complemented with centimetre observations from the Ryle Telescope, at 15 GHz (2.0 cm) and from the Green Bank Interferometer at 2.25 and 8.3 GHz (13 and 3.6 cm). Both Cygnus X-3 and SS 433 underwent moderate flaring events during our observations, whose main spectral evolution properties are described and interpreted. A significant spectral steepening was observed in both sources during the flare decay, that is likely to be caused by adiabatic expansion, inverse Compton and synchrotron losses. Finally, we also report 250 GHz upper limits for three additional undetected X-ray binary stars: LSI+65 010, LSI+61 235 and X Per.

  8. Dynamic nuclear polarization studies on deuterated nitroxyl spin probes.

    Science.gov (United States)

    David Jebaraj, D; Utsumi, Hideo; Milton Franklin Benial, A

    2017-10-01

    Detailed dynamic nuclear polarization and electron spin resonance studies were carried out for 3-carbamoyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl, 3-carboxy-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl,3-methoxycarbonyl-2,2,5,5-tetramethy pyrolidine-1-oxyl nitroxyl radicals and their corresponding deuterated nitroxyl radicals, used in Overhauser-enhanced magnetic resonance imaging for the first time. The dynamic nuclear polarization parameters such as dynamic nuclear polarization (DNP) factor, longitudinal relaxivity, saturation parameter, leakage factor and coupling factor were estimated for deuterated nitroxyl radicals. DNP enhancement increases with agent concentration up to 3 mm and decreases above 3 mm. The proton spin-lattice relaxation time and the longitudinal relaxivity parameters were estimated. The leakage factor increases with increasing agent concentration up to 3 mm and reaches plateau in the region 3-5 mm. The coupling parameter shows the interaction between the electron and nuclear spins to be mainly dipolar in origin. DNP spectrum exhibits that the full width at half maximum values are higher for undeuterated nitroxyl radicals compared with deuterated nitroxyl radicals, which leads to the increase in DNP enhancement. The ESR parameters such as, the line width, line shape, signal intensity ratio, rotational correlation time, hyperfine coupling constant and g-factor were calculated. The narrow line width was observed for deuterated nitroxyl radicals compared with undeuterated nitroxyl radicals, which leads to the higher saturation parameter value and DNP enhancement. The novelty of the work permits clear understanding of the DNP parameters determining the higher DNP enhancement compared with the undeuterated nitroxyl radicals. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

  9. Dynamic Nuclear Polarization enhanced NMR at 187 GHz/284 MHz using an Extended Interaction Klystron amplifier

    Science.gov (United States)

    Kemp, Thomas F.; Dannatt, Hugh R. W.; Barrow, Nathan S.; Watts, Anthony; Brown, Steven P.; Newton, Mark E.; Dupree, Ray

    2016-04-01

    A Dynamic Nuclear Polarisation (DNP) enhanced solid-state Magic Angle Spinning (MAS) NMR spectrometer which uses a 187 GHz (corresponding to 1H NMR frequency of 284 MHz) Extended Interaction Klystron (EIK) amplifier as the microwave source is briefly described. Its performance is demonstrated for a biomolecule (bacteriorhodopsin), a pharmaceutical, and surface functionalised silica. The EIK is very compact and easily incorporated into an existing spectrometer. The bandwidth of the amplifier is sufficient that it obviates the need for a sweepable magnetic field, once set, for all commonly used radicals. The variable power (CW or pulsed) output from the EIK is transmitted to the DNP-NMR probe using a quasi-optic system with a high power isolator and a corrugated waveguide which feeds the microwaves into the DNP-NMR probe. Curved mirrors inside the probe project the microwaves down the axis of the MAS rotor, giving a very efficient system such that maximum DNP enhancement is achieved with less than 3 W output from the microwave source. The DNP-NMR probe operates with a sample temperature down to 90 K whilst spinning at 8 kHz. Significant enhancements, in excess of 100 for bacteriorhodopsin in purple membrane (bR in PM), are shown along with spectra which are enhanced by ≈25 with respect to room temperature, for both the pharmaceutical furosemide and surface functionalised silica. These enhancements allow hitherto prohibitively time consuming experiments to be undertaken. The power at which the DNP enhancement in bR in PM saturates does not change significantly between 90 K and 170 K even though the enhancement drops by a factor of ≈11. As the DNP build up time decreases by a factor 3 over this temperature range, the reduction in T1n is presumably a significant contribution to the drop in enhancement.

  10. Endomorphin-2 Inhibition of Substance P Signaling within Lamina I of the Spinal Cord Is Impaired in Diabetic Neuropathic Pain Rats

    Science.gov (United States)

    Wan, Fa-Ping; Bai, Yang; Kou, Zhen-Zhen; Zhang, Ting; Li, Hui; Wang, Ya-Yun; Li, Yun-Qing

    2017-01-01

    Opiate analgesia in the spinal cord is impaired in diabetic neuropathic pain (DNP), but until now the reason is unknown. We hypothesized that it resulted from a decreased inhibition of substance P (SP) signaling within the dorsal horn of the spinal cord. To investigate this possibility, we evaluated the effects of endomorphin-2 (EM2), an endogenous ligand of the μ-opioid receptor (MOR), on SP release within lamina I of the spinal dorsal horn (SDH) in rats with DNP. We established the DNP rat model and compared the analgesic efficacy of EM2 between inflammation pain and DNP rat models. Behavioral results suggested that the analgesic efficacy of EM2 was compromised in the condition of painful diabetic neuropathy. Then, we measured presynaptic SP release induced by different stimulating modalities via neurokinin-1 receptor (NK1R) internalization. Although there was no significant change in basal and evoked SP release between control and DNP rats, EM2 failed to inhibit SP release by noxious mechanical and thermal stimuli in DNP but not in control and inflammation pain model. We also observed that EM2 decreased the number of FOS-positive neurons within lamina I of the SDH but did not change the amount of FOS/NK1R double-labeled neurons. Finally, we identified a remarkable decrease in MORs within the primary afferent fibers and dorsal root ganglion (DRG) neurons by Western blot (WB) and immunohistochemistry (IHC). Taken together, these data suggest that reduced presynaptic MOR expression might account for the loss of the inhibitory effect of EM2 on SP signaling, which might be one of the neurobiological foundations for decreased opioid efficacy in the treatment of DNP. PMID:28119567

  11. Stereospecific capillary electrophoresis assays using pentapeptide substrates for the study of Aspergillus nidulans methionine sulfoxide reductase A and mutant enzymes.

    Science.gov (United States)

    Zhu, Qingfu; El-Mergawy, Rabab G; Zhou, Yuzhen; Chen, Chunyang; Heinemann, Stefan H; Schönherr, Roland; Robaa, Dina; Sippl, Wolfgang; Scriba, Gerhard K E

    2016-07-01

    Stereospecific capillary electrophoresis-based methods for the analysis of methionine sulfoxide [Met(O)]-containing pentapeptides were developed in order to investigate the reduction of Met(O)-containing peptide substrates by recombinant Aspergillus nidulans methionine sulfoxide reductase A (MsrA) as well as enzymes carrying mutations in position Glu99 and Asp134. The separation of the diastereomers of the N-acetylated, C-terminally 2,4-dinitrophenyl (Dnp)-labeled pentapeptides ac-Lys-Phe-Met(O)-Lys-Lys-Dnp, ac-Lys-Asp-Met(O)-Asn-Lys-Dnp and ac-Lys-Asn-Met(O)-Asp-Lys-Dnp was achieved in 50 mM Tris-HCl buffers containing sulfated β-CD in fused-silica capillaries, while the diastereomer separation of ac-Lys-Asp-Met(O)-Asp-Lys-Dnp was achieved by sulfated β-CD-mediated MEKC. The methods were validated with regard to range, linearity, accuracy, limits of detection and quantitation as well as precision. Subsequently, the substrates were incubated with wild-type MsrA and three mutants in the presence of dithiothreitol as reductant. Wild-type MsrA displayed the highest activity towards all substrates compared to the mutants. Substitution of Glu99 by Gln resulted in the mutant with the lowest activity towards all substrates except for ac-Lys-Asn-Met(O)-Asp-Lys-Dnp, while replacement Asn for Asp134 lead to a higher activity towards ac-Lys-Asp-Met(O)-Asn-Lys-Dnp compared with the Glu99 mutant. The mutant with Glu instead of Asp134 was the most active among the mutant enzymes. Molecular modeling indicated that the conserved Glu99 residue is buried in the Met-S-(O) groove, which might contribute to the correct placing of substrates and, consequently, to the catalytic activity of MsrA, while Asp134 did not form hydrogen bonds with the substrates but only within the enzyme.

  12. Convergence and cross talk in urogenital neural circuitries.

    Science.gov (United States)

    Hubscher, C H; Gupta, D S; Brink, T S

    2013-10-01

    Despite common comorbidity of sexual and urinary dysfunctions, the interrelationships between the neural control of these functions are poorly understood. The medullary reticular formation (MRF) contributes to both mating/arousal functions and micturition, making it a good site to test circuitry interactions. Urethane-anesthetized adult Wistar rats were used to examine the impact of electrically stimulating different nerve targets [dorsal nerve of the penis (DNP) or clitoris (DNC); L6/S1 trunk] on responses of individual extracellularly recorded MRF neurons. The effect of bladder filling on MRF neurons was also examined, as was stimulation of DNP on bladder reflexes via cystometry. In total, 236 MRF neurons responded to neurostimulation: 102 to DNP stimulation (12 males), 64 to DNC stimulation (12 females), and 70 to L6/S1 trunk stimulation (12 males). Amplitude thresholds were significantly different at DNP (15.0 ± 0.6 μA), DNC (10.5 ± 0.7 μA), and L6/S1 trunk (54.2 ± 4.6 μA), whereas similar frequency responses were found (max responses near 30-40 Hz). In five males, filling/voiding cycles were lengthened with DNP stimulation (11.0 ± 0.9 μA), with a maximal effective frequency plateau beginning at 30 Hz. Bladder effects lasted ≈ 2 min after DNP stimulus offset. Many MRF neurons receiving DNP/DNC input responded to bladder filling (35.0% and 68.3%, respectively), either just before (43%) or simultaneously with (57%) the voiding reflex. Taken together, MRF-evoked responses with neurostimulation of multiple nerve targets along with different responses to bladder infusion have implications for the role of MRF in multiple aspects of urogenital functions.

  13. Inhibitory effect of dendroaspis natriuretic peptide on spontaneous contraction in gastric antral circular smooth muscles of guinea pigs

    Institute of Scientific and Technical Information of China (English)

    Hui-shu GUO; Zheng-xu CAI; Tai-hua WU; Jing XU; Yang QIU; Wen-xie XU

    2007-01-01

    Aim:To determine whether the natriuretic peptide receptor (NPR) is present in the stomach of guinea pigs and to investigate the effect of dendroaspis natriuretic peptide (DNP) on the gastric motility of guinea pigs and its mechanism. Methods:The distribution of the NPR was analyzed by autoradioimmunography. The spontaneous contraction of gastric antral circular muscles of guinea pigs was recorded by a 4-channel physiograph. The whole cell patch-clamp technique was introduced to record calcium-activated potassium currents in the gastric myocytes isolated by collagenase. Results:The NPR existed in the gastric fundus,gastric body,and gastric antrum of guinea pigs,and its density was largest in the gastric antrum. DNP inhibited spontaneous contraction and exhibited a dose-dependent manner. The DNP-induced inhibition was diminished by LY83583 (a guanylate cyclase inhibitor) and was potentiated by zaprinast (a cGMP-sensitive phosphoesterase inhibitor). The inhibitory effect of DNP on spontaneous contraction was also inhibited by tetraethylammonium (a non-selective potassium channel blocker);10 nmol/L DNP increased the calcium-activated potassium currents in the gastric circular myocytes of guinea pigs. Conclusion:The NPR is most common in the gastric antrum of guinea pigs. DNP significantly inhibits gastric motility in the gastric antrum of guinea pigs. The inhibitory effect occurs via a cGMP-dependent pathway,and a calcium-activated potassium channel may be also involved in the relaxation induced by DNP in gastric antral circular smooth muscles.

  14. Fast ion millimeter wave collective Thomson scattering diagnostics on TEXTOR and ASDEX upgrades

    DEFF Research Database (Denmark)

    Michelsen, S.; Korsholm, Søren Bang; Bindslev, H.

    2004-01-01

    Collective Thomson scattering (CTS) diagnostic systems for measuring fast ions in TEXTOR and ASDEX Upgrade are described in this article. Both systems use millimeter waves generated by gyrotrons as probing radiation and the scattered radiation is detected with heterodyne receivers having 40...

  15. Commissioning activities and first results from the collective Thomson scattering diagnostic on ASDEX Upgrade (invited)

    DEFF Research Database (Denmark)

    Meo, Fernando; Bindslev, Henrik; Korsholm, Søren Bang

    2008-01-01

    The collective Thomson scattering (CTS) diagnostic installed on ASDEX Upgrade uses millimeter waves generated by the newly installed 1 MW dual frequency gyrotron as probing radiation at 105 GHz. It measures backscattered radiation with a heterodyne receiver having 50 channels (between 100 and 110...

  16. Fast-ion dynamics in the TEXTOR tokamak measured by collective Thomson scattering

    DEFF Research Database (Denmark)

    Bindslev, H.; Nielsen, S.K.; Porte, L.;

    2006-01-01

    Here we present the first measurements by collective Thomson scattering of the evolution of fast-ion populations in a magnetically confined fusion plasma. 150 kW and 110 Ghz radiation from a gyrotron were scattered in the TEXTOR tokamak plasma with energetic ions generated by neutral beam injecti...

  17. EC power sources: European technological developments towards ITER

    Energy Technology Data Exchange (ETDEWEB)

    Bonicelli, T. [EFDA Close Support Unit Garching, Boltzmannstr 2, 85748 Garching (Germany)], E-mail: tullio.bonicelli@tech.efda.org; Alberti, S. [EPFL-CRPP, Association Euratom-Confederation Suisse, CH-1015 Lausanne (Switzerland); Cirant, S. [Istituto di Fisica del Plasma EURATOM-ENEA-CNR Association, Milan (Italy); Dormicchi, O. [Ansaldo Superconduttori SpA, C.so Perrone, Genoa (Italy); Fasel, D.; Hogge, J.P. [EPFL-CRPP, Association Euratom-Confederation Suisse, CH-1015 Lausanne (Switzerland); Illy, S.; Jin, J. [Forschungszentrum Karlsruhe, Association EURATOM FZK, IHM, 76344 Eggenstein-Leopoldshafen (Germany); Lievin, C. [Thales Electron Devices (TED), 2 Rue de Latecoere, F-78141 Velizy-Villacoublay (France); Mondino, P.L. [EFDA Close Support Unit Garching, Boltzmannstr 2, 85748 Garching (Germany); Piosczyk, B.; Rzesnicki, T. [Forschungszentrum Karlsruhe, Association EURATOM FZK, IHM, 76344 Eggenstein-Leopoldshafen (Germany); Santinelli, M. [ENEA Frascati, Via E Fermi 45, 00044 Frascati, Rome (Italy); Taddia, G. [OCEM, Via 2 Agosto 1980 11, 40016 San Giorgio di Piano (Italy); Thumm, M. [Forschungszentrum Karlsruhe, Association EURATOM FZK, IHM, 76344 Eggenstein-Leopoldshafen (Germany); Tran, M.Q. [EPFL-CRPP, Association Euratom-Confederation Suisse, CH-1015 Lausanne (Switzerland)

    2007-10-15

    The activities in Europe towards the development of the EC power sources for ITER are centered on the development of a 170 GHz, 2 MW, CW coaxial cavity gyrotron of collector potential depressed (CPD) type. A gyrotron with a higher unit power than the ITER reference (1 MW) would yield a reduction of the installation costs, a more compact launcher design and, if required, an increase of the power delivered through one port. Tests proving the principle were successfully performed on a short-pulse experimental gyrotron delivering up to 2.2 MW in single mode. Following this success, a coordinated and fully consistent programme of development has been launched. The first industrial 2 MW prototype is now at an advanced stage of construction. The associated superconductive magnet producing 6.86 T on the cavity axis is also being procured. Dummy loads suitable for short and CW operation are also part of the development effort. Finally, a new EC test facility, with the features necessary for the testing of the gyrotron up to full power in CW, has been established and includes a fully solid-state power supply system.0.

  18. Review of Negative Hydrogen Ion Sources

    Science.gov (United States)

    1990-09-01

    is v -senritiv-t-t-ih- -tliffe•p- asma -sffi-ei onverter surface which is biased negative -dfikensions and confitiioS in thigregion.0 ]t ihe-extitifoin...National Laboratory, Accelerator Technology Division, MS H85 1, Los Alamos, New Mexico , 87545 ABSTRACT The Large Orbit Gyrotron Los Alamos is investigating

  19. Three-wave interaction during electron cyclotron resonance heating and current drive

    DEFF Research Database (Denmark)

    Nielsen, Stefan Kragh; Jacobsen, Asger Schou; Hansen, Søren Kjer

    2016-01-01

    Non-linear wave-wave interactions in fusion plasmas, such as the parametric decay instability (PDI) of gyrotron radiation, can potentially hamper the use of microwave diagnostics. Here we report on anomalous scattering in the ASDEX Upgrade tokamak during electron cyclotron resonance heating...

  20. Annual Industrial Capabilities Report to Congress

    Science.gov (United States)

    2009-03-01

    The System Development and Demonstration Phase of the Joint Tactical Radio System Airborne Maritime/Fixed (JTRS AMF ) Cluster continues through FY11...that will use HTS coated conductor include: gyrotron magnets, power generators, power converters and 98 transformers, motors, primary power cabling

  1. Electron cyclotron resonance heating on TEXTOR

    NARCIS (Netherlands)

    Westerhof, E.; Hoekzema, J. A.; Hogeweij, G. M. D.; Jaspers, R. J. E.; Schüller, F. C.; Barth, C. J.; Bongers, W. A.; Donne, A. J. H.; Dumortier, P.; van der Grift, A. F.; van Gorkom, J. C.; Kalupin, D.; Koslowski, H. R.; Kramer-Flecken, A.; Kruijt, O. G.; Cardozo, N. J. L.; Mantica, P.; van der Meiden, H. J.; Merkulov, A.; Messiaen, A.; Oosterbeek, J. W.; Oyevaar, T.; Poelman, A. J.; Polman, R. W.; Prins, P. R.; Scholten, J.; Sterk, A. B.; Tito, C. J.; Udintsev, V.S.; Unterberg, B.; Vervier, M.; van Wassenhove, G.

    2003-01-01

    The 110 GHz and the new 140 GHz gyrotron systems for electron cyclotron resonance heating (ECRH) and ECCD on TEXTOR are described and results of ECRH experiments with the 110 GHz system are reported. Central ECRH on Ohmic plasmas shows the presence of an internal electron transport barrier near q =

  2. Spectrum response and analysis of 77 GHz band collective Thomson scattering diagnostic for bulk and fast ions in LHD plasmas

    DEFF Research Database (Denmark)

    Nishiura, M.; Kubo, S.; Tanaka, K.

    2014-01-01

    A collective Thomson scattering (CTS) diagnostic was developed and used to measure the bulk and fast ions originating from 180 keV neutral beams in the Large Helical Device (LHD). Electromagnetic waves from a gyrotron at 77 GHz with 1 MW power output function as both the probe and electron cyclot...

  3. Contributions to resolving issues impeding the operation of high power microwave devices

    Science.gov (United States)

    Kashyn, Dmytro

    This thesis reports an experimental study aimed at extending high power, high efficiency gyrotron operation to submillimeter wavelengths. A series of experiments carried out both at the University of Maryland and the Institute of Applied Physics of the Russian Academy of Science, succeeded in demonstrating output power at 670 GHz of 180 kilowatts with 20% efficiency (gyrotron voltage was 57 kV and beam current was 16 amperes). The maximum output power achieved in the experiments was 210kW at somewhat higher voltage and current (viz. 58kV and 22A). The achieved output power and efficiency are twice as large as achieved in previous experiments in this frequency range with pulse duration in the range of tens of microseconds. These performance parameters are relevant to a previously proposed application of detecting concealed radioactive materials by air breakdown in a focused beam of sub-millimeter radiation. The 670 GHz gyrotron combined features of two lines of previous experiments: (a) to operate at the required frequency, pulsed solenoids producing 28T magnetic were employed and (b) to obtain high efficiency a very high order mode was used in the gyrotron cavity, as in the experiments with gyrotrons for plasma heating. Evidence of multimode beating was observed in submillimeter output envelope. The excitation of spurious modes, especially during the rise of the gyrotron voltage pulse, was analyzed and the method of avoiding this was proposed which also allows to reduce collector loading in gyrotrons operating in modulated regimes. The present study also includes theoretical analysis of the processes that deepens the understanding of microwave breakdown (arcing) in high power microwave devices. The effect of the dust particles microprotrusions on the device operation was analyzed. These microprotrusions were observed and their negative effects were remedied by careful polishing and machining of the resonator surface. Finally, the generated 670 GHz radiation was

  4. Dynamic nuclear polarization properties of nitroxyl radical in high viscous liquid using Overhauser-enhanced Magnetic Resonance Imaging (OMRI).

    Science.gov (United States)

    Kumara Dhas, M; Utsumi, Hideo; Jawahar, A; Milton Franklin Benial, A

    2015-08-01

    The dynamic nuclear polarization (DNP) studies were carried out for (15)N labeled carbamoyl-PROXYL in pure water and pure water/glycerol mixtures of different viscosities (1.8cP, 7cP and 14cP). The dependence of DNP parameters was demonstrated over a range of agent concentration, viscosities, RF power levels and ESR irradiation time. DNP spectra were also recorded for 2mM concentration of (15)N labeled carbamoyl-PROXYL in pure water and pure water/glycerol mixtures of different viscosities. The DNP factors were measured as a function of ESR irradiation time, which increases linearly up to 2mM agent concentration in pure water and pure water/glycerol mixtures of different viscosities. The DNP factor started declining in the higher concentration region (∼3mM), which is due to the ESR line width broadening. The water proton spin-lattice relaxation time was measured at very low Zeeman field (14.529mT). The increased DNP factor (35%) was observed for solvent 2 (η=1.8cP) compared with solvent 1 (η=1cP). The increase in the DNP factor was brought about by the shortening of water proton spin-lattice relaxation time of solvent 2. The decreased DNP factors (30% and 53%) were observed for solvent 3 (η=7cP) and solvent 4 (η=14cP) compared with solvent 2, which is mainly due to the low value of coupling parameter in high viscous liquid samples. The longitudinal relaxivity, leakage factor and coupling parameter were estimated. The coupling parameter values reveal that the dipolar interaction as the major mechanism. The longitudinal relaxivity increases with the increasing viscosity of pure water/glycerol mixtures. The leakage factor showed an asymptotic increase with the increasing agent concentration. It is envisaged that the results reported here may provide guidelines for the design of new viscosity prone nitroxyl radicals, suited to the biological applications of DNP. Copyright © 2015 Elsevier Inc. All rights reserved.

  5. Hiring and incorporating doctor of nursing practice-prepared nurse faculty into academic nursing programs.

    Science.gov (United States)

    Agger, Charlotte A; Oermann, Marilyn H; Lynn, Mary R

    2014-08-01

    Semistructured interviews were conducted with 15 deans and directors of nursing programs across the United States to gain an understanding of how Doctor of Nursing Practice (DNP)-prepared nurses seeking academic positions are hired and used in schools of nursing. Interviews sought to gain information regarding (a) differences and similarities in the roles and responsibilities of DNP- and Doctor of Philosophy (PhD)-prepared faculty, (b) educational advancement and mentoring of DNP-prepared nurse faculty, (c) recruitment of doctorally prepared nurse faculty, and (d) shortages of nursing faculty. DNP- and PhD-prepared nurse faculty are hired for varying roles in baccalaureate and higher degree schools of nursing, some similar to other faculty with master's degrees and others similar to those with PhDs; in associate degree in nursing programs, they are largely hired for the same type of work as nurse faculty with master's degrees. Regardless of program or degree type, the main role of DNP-prepared faculty is teaching. Copyright 2014, SLACK Incorporated.

  6. Preparing practice scholars: teaching knowledge application in the Doctor of Nursing Practice curriculum.

    Science.gov (United States)

    Buchholz, Susan Weber; Budd, Geraldine M; Courtney, Maureen R; Neiheisel, Mary B; Hammersla, Margaret; Carlson, Elizabeth D

    2013-09-01

    The purpose of this article is to explore the scholarship role of the Doctor of Nursing Practice (DNP) and the associated knowledge and skills required for success. There are excellent competencies provided by national organizations that present guidelines for design and application of this practice scholar's contributions. Although evidence-based research translation is known to be essential for the DNP scholar, a consensus does not exist about the required research knowledge and skill levels that are needed. A model was developed to depict the scholarship roles of the DNP and the Doctor of Philosophy (PhD). This model indicates both DNP and PhD scholars are alike in their enactment of active scholarship but have different areas of expertise. They are different in their major roles that lead to the development of practice science; the DNP is the expert in knowledge application while the PhD is the expert in knowledge generation. A nurse practice scholar needs to have a fundamental and strong understanding of research design and interpretation in order to appraise and implement research-based evidence into practice and conduct clinical projects. ©2013 The Author(s) ©2013 American Association of Nurse Practitioners.

  7. Structural Analysis and Immuno-Stimulating Activity of an Acidic Polysaccharide from the Stems of Dendrobium nobile Lindl.

    Science.gov (United States)

    Wang, Jun-Hui; Zuo, Shu-Rong; Luo, Jian-Ping

    2017-04-10

    Dendrobium nobile Lindl., an epiphytic herb distributed in the Southeast Asia, is used as a tonic and antipyretic herbal medicine in China. In this study, a water-soluble acidic heteropolysaccharide, DNP-W4, containing mannose, glucose, galactose, xylose, rhamnose, and galacturonic acid, in the molar ratios of 1.0:4.9:2.5:0.5:1.0:0.9, was obtained from the stems of Dendrobium nobile Lindl. Using methylation analysis, partial acid hydrolysis, pectolyase treatment, NMR, and ESI-MS, the structure of DNP-W4 was elucidated. The obtained data indicated that DNP-W4 was a complex heteropolysaccharide and possessed a backbone composed of (1→4)-linked β-d-Glcp, (1→6)-linked β-d-Glcp, and (1→6)-linked β-d-Galp, with substitutes at O-4/6 of Glcp residues and O-3 of Galp. The branches of DNP-W4 were composed of terminal Manp, (1→6)-linked β-d-Manp, (1→3)-linked β-d-Glcp, β-d-Glcp, β-d-Galp, (1→4)-linked α-d-GalAp, (1→2)-linked α-L-Rhap, and Xylp. DNP-W4 had little immunological activities, but its derivatives had immuno-stimulating activities to some extent.

  8. Stabilization of endogenous Nrf2 by minocycline protects against Nlrp3-inflammasome induced diabetic nephropathy

    Science.gov (United States)

    Shahzad, Khurrum; Bock, Fabian; Al-Dabet, Moh’d Mohanad; Gadi, Ihsan; Nazir, Sumra; Wang, Hongjie; Kohli, Shrey; Ranjan, Satish; Mertens, Peter R.; Nawroth, Peter P.; Isermann, Berend

    2016-01-01

    While a plethora of studies support a therapeutic benefit of Nrf2 activation and ROS inhibition in diabetic nephropathy (dNP), the Nrf2 activator bardoxolone failed in clinical studies in type 2 diabetic patients due to cardiovascular side effects. Hence, alternative approaches to target Nrf2 are required. Intriguingly, the tetracycline antibiotic minocycline, which has been in clinical use for decades, has been shown to convey anti-inflammatory effects in diabetic patients and nephroprotection in rodent models of dNP. However, the mechanism underlying the nephroprotection remains unknown. Here we show that minocycline protects against dNP in mouse models of type 1 and type 2 diabetes, while caspase -3,-6,-7,-8 and -10 inhibition is insufficient, indicating a function of minocycline independent of apoptosis inhibition. Minocycline stabilizes endogenous Nrf2 in kidneys of db/db mice, thus dampening ROS-induced inflammasome activation in the kidney. Indeed, minocycline exerts antioxidant effects in vitro and in vivo, reducing glomerular markers of oxidative stress. Minocycline reduces ubiquitination of the redox-sensitive transcription factor Nrf2 and increases its protein levels. Accordingly, minocycline mediated Nlrp3 inflammasome inhibition and amelioration of dNP are abolished in diabetic Nrf2−/− mice. Taken together, we uncover a new function of minocycline, which stabilizes the redox-sensitive transcription factor Nrf2, thus protecting from dNP. PMID:27721446

  9. Pulsed Dynamic Nuclear Polarization with Trityl Radicals.

    Science.gov (United States)

    Mathies, Guinevere; Jain, Sheetal; Reese, Marcel; Griffin, Robert G

    2016-01-07

    Continuous-wave (CW) dynamic nuclear polarization (DNP) is now established as a method of choice to enhance the sensitivity in a variety of NMR experiments. Nevertheless, there remains a need for the development of more efficient methods to transfer polarization from electrons to nuclei. Of particular interest are pulsed DNP methods because they enable a rapid and efficient polarization transfer that, in contrast with CW DNP methods, is not attenuated at high magnetic fields. Here we report nuclear spin orientation via electron spin-locking (NOVEL) experiments using the polarizing agent trityl OX063 in glycerol/water at a temperature of 80 K and a magnetic field of 0.34 T. (1)H NMR signal enhancements up to 430 are observed, and the buildup of the local polarization occurs in a few hundred nanoseconds. Thus, NOVEL can efficiently dynamically polarize (1)H atoms in a system that is of general interest to the solid-state DNP NMR community. This is a first, important step toward the general application of pulsed DNP at higher fields.

  10. Production and characterisation of polyclonal antisera against feline IgE.

    Science.gov (United States)

    Gilbert, S; Halliwell, R E

    1998-05-29

    Cats, naturally or experimentally infected with Toxocara cati were immunised with dinitrophenylated ascaris antigen (DNP-Asc). All cats developed immediate skin reactivity to DNP coupled to bovine serum albumin (DNP-BSA) and the sera of the nine cats had a heat labile homocytotropic antibody detectable by homologous Prausnitz-Küstner (PK) tests. Reagin-rich fractions were prepared from these sera and used for the preparation of polyclonal antisera in rabbits. Resultant antisera were passed through a immunoabsorbent column of Sepharose 4B coupled to heated normal cat serum. An immunoabsorbent column prepared with the resultant antisera removed the PK reactivity from the cat sera, and the activity was recovered following acid elution. The antiserum failed to detect any recognised immunoglobulin in cat sera, but precipitated with a heat labile protein with gamma-1 electrophoretic mobility in the sera of parasited cats. These findings support the contention that the antisera are specific for feline IgE.

  11. Reactive surface organometallic complexes observed using dynamic nuclear polarization surface enhanced NMR spectroscopy

    KAUST Repository

    Pump, Eva

    2016-08-15

    Dynamic Nuclear Polarization Surface Enhanced NMR Spectroscopy (DNP SENS) is an emerging technique that allows access to high-sensitivity NMR spectra from surfaces. However, DNP SENS usually requires the use of radicals as an exogenous source of polarization, which has so far limited applications for organometallic surface species to those that do not react with the radicals. Here we show that reactive surface species can be studied if they are immobilized inside porous materials with suitably small windows, and if bulky nitroxide bi-radicals (here TEKPol) are used as the polarization source and which cannot enter the pores. The method is demonstrated by obtaining significant DNP enhancements from highly reactive complelxes [(equivalent to Si-O-)W(Me)(5)] supported on MCM-41, and effects of pore size (6.0, 3.0 and 2.5 nm) on the performance are discussed.

  12. Multimethod teaching strategies to integrate selected QSEN competencies in a Doctor of Nursing Practice distance education program.

    Science.gov (United States)

    Manning, Mary Lou; Frisby, Anthony J

    2011-01-01

    The Quality and Safety Education for Nurses (QSEN) initiative identified 6 competencies for the education of nurses (patient-centered care, teamwork and collaboration, evidence-based practice, quality improvement, safety, and informatics) and the related knowledge, skills, and attitudes (KSAs) for each competency. The initial QSEN focus was on competency development during prelicensure nursing education, with subsequent attention on adapting the KSAs for graduate programs that prepare advanced practice nurses for clinical roles. Description of successful QSEN competency integration in Doctor of Nursing Practice (DNP) programs is limited. Although the ultimate goal is executing DNP programs where quality and safety is thoroughly integrated throughout the curricula, the focus of this article is on multimethod teaching strategies to integrate selected QSEN KSAs into an existing online post-master's DNP quality and safety course.

  13. Mild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.

    Directory of Open Access Journals (Sweden)

    Fernanda M Cerqueira

    Full Text Available Enhanced mitochondrial biogenesis promoted by eNOS activation is believed to play a central role in the beneficial effects of calorie restriction (CR. Since treatment of mice with dinitrophenol (DNP promotes health and lifespan benefits similar to those observed in CR, we hypothesized that it could also impact biogenesis. We found that DNP and CR increase citrate synthase activity, PGC-1α, cytochrome c oxidase and mitofusin-2 expression, as well as fasting plasma levels of NO• products. In addition, eNOS and Akt phosphorylation in skeletal muscle and visceral adipose tissue was activated in fasting CR and DNP animals. Overall, our results indicate that systemic mild uncoupling activates eNOS and Akt-dependent pathways leading to mitochondrial biogenesis.

  14. Instrumentation for cryogenic magic angle spinning dynamic nuclear polarization using 90L of liquid nitrogen per day.

    Science.gov (United States)

    Albert, Brice J; Pahng, Seong Ho; Alaniva, Nicholas; Sesti, Erika L; Rand, Peter W; Saliba, Edward P; Scott, Faith J; Choi, Eric J; Barnes, Alexander B

    2017-10-01

    Cryogenic sample temperatures can enhance NMR sensitivity by extending spin relaxation times to improve dynamic nuclear polarization (DNP) and by increasing Boltzmann spin polarization. We have developed an efficient heat exchanger with a liquid nitrogen consumption rate of only 90L per day to perform magic-angle spinning (MAS) DNP experiments below 85K. In this heat exchanger implementation, cold exhaust gas from the NMR probe is returned to the outer portion of a counterflow coil within an intermediate cooling stage to improve cooling efficiency of the spinning and variable temperature gases. The heat exchange within the counterflow coil is calculated with computational fluid dynamics to optimize the heat transfer. Experimental results using the novel counterflow heat exchanger demonstrate MAS DNP signal enhancements of 328±3 at 81±2K, and 276±4 at 105±2K. Copyright © 2017 Elsevier Inc. All rights reserved.

  15. Environmental conditions influence the plant functional diversity effect on potential denitrification.

    Directory of Open Access Journals (Sweden)

    Ariana E Sutton-Grier

    Full Text Available Global biodiversity loss has prompted research on the relationship between species diversity and ecosystem functioning. Few studies have examined how plant diversity impacts belowground processes; even fewer have examined how varying resource levels can influence the effect of plant diversity on microbial activity. In a field experiment in a restored wetland, we examined the role of plant trait diversity (or functional diversity, (FD and its interactions with natural levels of variability of soil properties, on a microbial process, denitrification potential (DNP. We demonstrated that FD significantly affected microbial DNP through its interactions with soil conditions; increasing FD led to increased DNP but mainly at higher levels of soil resources. Our results suggest that the effect of species diversity on ecosystem functioning may depend on environmental factors such as resource availability. Future biodiversity experiments should examine how natural levels of environmental variability impact the importance of biodiversity to ecosystem functioning.

  16. Development on dynamic nuclear polarized targets

    CERN Document Server

    Penttila, S I

    2002-01-01

    Our interest in understanding the spin content of the nucleon has left its marks on the recent development of the dynamic nuclear polarized (DNP) targets. This can be seen from the targets developed at CERN and SLAC for the measurement of the polarized spin structure functions in deep inelastic scattering. The results of the experiments indicated that less than 30% of the nucleon spin is carried by the quarks. This unpredicted small value initiated planning of new polarized target experiments to determine the gluon polarization on the nucleon using polarized real photons and polarized /sup 6/LiD targets. In several facilities very intense polarized photon beams are available at a wide energy range. During the next few years these photon beams with DNP targets will be used to test the fundamental GDH sum rule. Other DNP target developments are also discussed. (61 refs).

  17. H-1 Dynamic Nuclear Polarization in Supercritical Ethylene at 1.4 T

    Energy Technology Data Exchange (ETDEWEB)

    Wind, Robert A.(BATTELLE (PACIFIC NW LAB)); Shi, Bai (PNNL); Hu, Jian Zhi (BATTELLE (PACIFIC NW LAB)); Solum, Mark S.(Utah, Univ Of); Ellis, Paul D.(BATTELLE (PACIFIC NW LAB)); Grant, David M.(Utah, Univ Of); Pugmire, Ronald J.(Utah, Univ Of); Taylor, Craig M.(DOE); Yonker, Clement R.(BATTELLE (PACIFIC NW LAB))

    2000-03-01

    H1 dynamic nuclear polarization (DNP) has been measured in supercritical ethylene in the pressure range 60-300 bar and in an external field of 1.4 T. A single-cell sapphire tube was used as a high pressure cell and powdered 1,3-bisdiphenylene-2-phenyl allyl(BDPA) free radicals were added and distributed at the wall of the cell. At all pressures the dominant DNP effect was a positive Overhauser enhancement, caused by proton-electron contact interactions at the fluid/solid radical interface. The observed enhancements varied from 12 at 67 bar to 17 at 300 bar. Besides the Overhauser enhancement, also a small solid state and thermal mixing enhancement were observed, indicating that part of the ethylene is absorbed at the radical surface for a prolonged time. These data indicate that DNP-enhanced NMR has the potential of extending the impact of NMR in research areas involving supercritical fluids.

  18. Doctoral education for WOC nurses considering advanced practice nursing.

    Science.gov (United States)

    Pieper, Barbara; Colwell, Janice

    2012-01-01

    Advanced practice nursing education is at a crossroads. Societal changes, increased health care demands, and leadership nursing organizations have identified the need of a doctor of nursing practice (DNP) degree as the advanced practice degree. WOC nurses need to examine DNP programs when considering returning for an advanced practice degree. This article explores nursing education at the doctorate level and areas the WOC nurse should consider when making a decision about attending a program. The WOC nurse needs to understand the similarities and differences of the doctor of philosophy and the DNP, issues about each program and its completion, personal factors, and the application process. Although selecting a doctoral program is a daunting experience, the education will provide opportunities for the WOC nurse to excel as a scholar, thus influencing the profession and the practice.

  19. New probes for angiotensin II receptors. Synthesis, radioiodination and biological properties of biotinylated and haptenated angiotensin derivatives.

    Science.gov (United States)

    Bonnafous, J C; Tence, M; Seyer, R; Marie, J; Aumelas, A; Jard, S

    1988-05-01

    The present work delineates the basis for chemical modifications which can be introduced on the angiotensin II (AII) molecule to design probes suitable for indirect affinity techniques, especially for receptor purification. Using the solid-phase synthesis strategy, biotin or dinitrophenyl moieties have been added at the N-terminus of AII, with aminohexanoic acid as spacer arm. The resulting probes, (6-biotinylamido)hexanoyl-AII (Bio-Ahx-AII) and dinitrophenylaminohexanoyl-AII (Dnp-Ahx-AII), were prepared in their monoiodinated and highly labelled radioiodinated forms, with possible sulphoxidation of biotin. In addition to their ability to interact with streptavidin and anti-Dnp antibodies respectively, the two ligands displayed almost unchanged affinities for hepatic AII receptors as compared with AII. Bio-Ahx-AII and Dnp-Ahx-AII behaved as agonists on several AII-sensitive systems. The potential applications of these probes, receptor purification, cell labelling and sorting and histochemical receptor visualization, are discussed.

  20. Development of Redox Metabolic Imaging Using Endogenous Molecules.

    Science.gov (United States)

    Hyodo, Fuminori; Ito, Shinji; Eto, Hinako; Nakaji, Tomoko; Yasukawa, Keiji; Kobayashi, Ryoma; Utsumi, Hideo

    2016-01-01

    Redox metabolism plays a central role in maintaining homeostasis in living organisms. The electron transfer system in mitochondria produces ATP via endogenous redox molecules such as flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD), and coenzyme Q10 (CoQ10), which have flavin or quinone moieties. One-electron transfer reactions convert FMN, FAD, and CoQ10 to the free radical intermediates FMNH and FADH, and CoQ10H, respectively. Dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) allows us to visualize free radicals in vitro and in vivo. We present a spectroscopic imaging technology with DNP-MRI, which enables the imaging of multiple free radical intermediates such as FADH and CoQH. DNP-MRI can also identify various endogenous free radical intermediates derived from redox transformations.

  1. Test of a theory relating to the cross-linking of IgE antibody on the surface of human basophils

    Energy Technology Data Exchange (ETDEWEB)

    MacGlashan, D.W. Jr.; Dembo, M.; Goldstein, B.

    1985-12-01

    Recent mathematical models of bivalent hapten-induced histamine release from basophils predict that under appropriate conditions histamine release is maximum when cross-link formation is maximum, at a hapten concentration equal to 1/(2K/sub a/), where K/sub a/ is the average affinity constant of the hapten for a single IgE binding site. To test this prediction the authors sensitized human basophils with a monoclonal anti-dinitrophenol IgE and generated histamine release dose-response curves with a bivalent hapten, ..cap alpha..,epsilon-DNP-lysine. The monoclonal IgE has a published affinity constant of 7.1 x 10/sup 7/ M/sup -1/ for epsilon-DNP-lysine as determined by equilibrium dialysis. From the position of the maximum of the histamine dose-response curves, both in the presence and in the absence of monovalent DNP hapten, the authors determine that the sensitizing IgE has an intrinsic affinity constant of 6.9 +/- 0.5 x 10/sup 7/ M/sup -1/ for such that-DNP-lysine and 1.2 +/- 0.6 x 10/sup 6/ M/sup -1/ for ..cap alpha..-DNP-lysine. The agreement between the two estimates of the epsilon-DNP-lysine affinity constant, one from histamine release experiments involving surface bound IgE and one from binding experiments involving IgE free in solution, 1) is consistent with a central prediction of the theory of cross-linking and 2) indicates that the hapten-binding properties of the IgE are unaffected by its being bound to Fc/sub epsilon/ receptors on the basophil surface. 30 references, 3 figures, 3 tables.

  2. Multiple metabolic hits converge on CD36 as novel mediator of tubular epithelial apoptosis in diabetic nephropathy.

    Directory of Open Access Journals (Sweden)

    Katalin Susztak

    2005-02-01

    Full Text Available BACKGROUND: Diabetic nephropathy (DNP is a common complication of type 1 and type 2 diabetes mellitus and the most common cause of kidney failure. While DNP manifests with albuminuria and diabetic glomerulopathy, its progression correlates best with tubular epithelial degeneration (TED and interstitial fibrosis. However, mechanisms leading to TED in DNP remain poorly understood. METHODS AND FINDINGS: We found that expression of scavenger receptor CD36 coincided with proximal tubular epithelial cell (PTEC apoptosis and TED specifically in human DNP. High glucose stimulated cell surface expression of CD36 in PTECs. CD36 expression was necessary and sufficient to mediate PTEC apoptosis induced by glycated albumins (AGE-BSA and CML-BSA and free fatty acid palmitate through sequential activation of src kinase, and proapoptotic p38 MAPK and caspase 3. In contrast, paucity of expression of CD36 in PTECs in diabetic mice with diabetic glomerulopathy was associated with normal tubular epithelium and the absence of tubular apoptosis. Mouse PTECs lacked CD36 and were resistant to AGE-BSA-induced apoptosis. Recombinant expression of CD36 in mouse PTECs conferred susceptibility to AGE-BSA-induced apoptosis. CONCLUSION: Our findings suggest a novel role for CD36 as an essential mediator of proximal tubular apoptosis in human DNP. Because CD36 expression was induced by glucose in PTECs, and because increased CD36 mediated AGE-BSA-, CML-BSA-, and palmitate-induced PTEC apoptosis, we propose a two-step metabolic hit model for TED, a hallmark of progression in DNP.

  3. Effect of duloxetine on expression of Toll-like receptor 4 in spinal dorsal horn in a rat model of dia-betic neuropathic pain%度洛西汀对糖尿病神经病理性痛大鼠脊髓背角Toll样受体4表达的影响

    Institute of Scientific and Technical Information of China (English)

    陈文君; 周冬梅; 苗蓓; 李伟

    2015-01-01

    目的:评价度洛西汀对糖尿病神经病理性痛大鼠脊髓背角Toll样受体4( TLR4)表达的影响。方法雄性SD大鼠,体重180~220 g,2月龄,采用高糖高脂饮食8周后腹腔注射链脲佐菌素( STZ)35 mg∕kg的方法制备2型糖尿病模型,取糖尿病神经病理性痛模型制备成功的大鼠75只,采用随机数字表法,将其分为5组( n=15):糖尿病神经病理性痛组( DNP 组)、DNP+生理盐水组( DNP+NS组)、DNP+不同剂量度洛西汀组( DNP+D5组、DNP+D10组和DNP+D20组)。另取正常大鼠15只作为对照组( C组)。 DNP+D5组、DNP+D10组和DNP+D20组于注射STZ后15 d时分别腹腔注射度洛西汀5、10和20 mg∕kg,1次∕d,连续14 d;DNP+NS组给予等容量生理盐水。于注射STZ前、注射STZ后14、17、21、28 d时测定机械缩足反应阈( MWT)和热缩足反应潜伏期( TWL)。于最后一次痛阈测定后,取L4⁃6节段脊髓,采用免疫组化法和Western blot法检测脊髓背角TLR4表达水平。结果与C组比较,DNP组、DNP+D5组、DNP+D10组和DNP+D20组MWT降低,TWL缩短,脊髓背角TLR4表达上调( P<0.05);与DNP组比较,DNP+D5组、DNP+D10组和DNP+D20组MWT升高,TWL延长,脊髓背角TLR4表达下调(P<0.05),DNP+NS组上述指标差异无统计学意义(P>0.05)。结论度洛西汀减轻大鼠糖尿病神经病理性痛的机制与下调脊髓背角TLR4表达有关。%Objective To evaluate the effect of duloxetine on the expression of Toll⁃like receptor 4 (TLR4) in the spinal dorsal horn in a rat model of diabetic neuropathic pain (DNP). Methods Type 2 di⁃abetes mellitus was induced by high⁃fat and high⁃sucrose diet and intraperitoneal streptozotocin ( STZ) 35 mg∕kg in male Sprague⁃Dawley rats, aged 2 months, weighing 180-220 g. Seventy⁃five rats with type 2 di⁃abetes mellitus were randomly divided into 5 groups ( n

  4. Dynamic nuclear polarization NMR spectroscopy allows high-throughput characterization of microporous organic polymers.

    Science.gov (United States)

    Blanc, Frédéric; Chong, Samantha Y; McDonald, Tom O; Adams, Dave J; Pawsey, Shane; Caporini, Marc A; Cooper, Andrew I

    2013-10-16

    Dynamic nuclear polarization (DNP) solid-state NMR was used to obtain natural abundance (13)C and (15)N CP MAS NMR spectra of microporous organic polymers with excellent signal-to-noise ratio, allowing for unprecedented details in the molecular structure to be determined for these complex polymer networks. Sensitivity enhancements larger than 10 were obtained with bis-nitroxide radical at 14.1 T and low temperature (∼105 K). This DNP MAS NMR approach allows efficient, high-throughput characterization of libraries of porous polymers prepared by combinatorial chemistry methods.

  5. Aged dominant negative p38α MAPK mice are resistant to age-dependent decline in adult-neurogenesis and context discrimination fear conditioning.

    Science.gov (United States)

    Cortez, IbDanelo; Bulavin, Dmitry V; Wu, Ping; McGrath, Erica L; Cunningham, Kathryn A; Wakamiya, Maki; Papaconstantinou, John; Dineley, Kelly T

    2017-03-30

    A major aspect of mammalian aging is the decline in functional competence of many self-renewing cell types, including adult-born neuronal precursors. Since age-related senescence of self-renewal occurs simultaneously with chronic up-regulation of the p38MAPKalpha (p38α) signaling pathway, we used the dominant negative mouse model for attenuated p38α activity (DN-p38α(AF/+)) in which Thr180 and Tyr182 are mutated (T→A/Y→F) to prevent phosphorylation activation (DN-p38α(AF/+)) and kinase activity. As a result, aged DN-p38α(AF/+) mice are resistant to age-dependent decline in proliferation and regeneration of several peripheral tissue progenitors when compared to wild-type littermates. Aging is the major risk factor for non-inherited forms of Alzheimer's disease (AD); environmental and genetic risk factors that accelerate the senescence phenotype are thought to contribute to an individual's relative risk. In the present study, we evaluated aged DN-p38α(AF/+) and wildtype littermates in a series of behavioral paradigms to test if p38α mutant mice exhibit altered baseline abnormalities in neurological reflexes, locomotion, anxiety-like behavior, and age-dependent cognitive decline. While aged DN-p38α(AF/+) and wildtype littermates appear equal in all tested baseline neurological and behavioral parameters, DN-p38α(AF/+) exhibit superior context discrimination fear conditioning. Context discrimination is a cognitive task that is supported by proliferation and differentiation of adult-born neurons in the dentate gyrus of the hippocampus. Consistent with enhanced context discrimination in aged DN-p38α(AF/+), we discovered enhanced production of adult-born neurons in the dentate gyrus of DN-p38α(AF/+) mice compared to wildtype littermates. Our findings support the notion that p38α inhibition has therapeutic utility in aging diseases that affect cognition, such as AD.

  6. Generating highly polarized nuclear spins in solution using dynamic nuclear polarization

    DEFF Research Database (Denmark)

    Wolber, J.; Ellner, F.; Fridlund, B.;

    2004-01-01

    and other low-γ nuclei. Subsequent to the DNP process, the solid sample is dissolved rapidly with a warm solvent to create a solution of molecules with highly polarized nuclear spins. Two main applications are proposed: high-resolution liquid state NMR with enhanced sensitivity, and the use......A method to generate strongly polarized nuclear spins in solution has been developed, using Dynamic Nuclear Polarization (DNP) at a temperature of 1.2K, and at a field of 3.354T, corresponding to an electron spin resonance frequency of 94GHz. Trityl radicals are used to directly polarize 13C...

  7. A uranium (VI) complex: Synthesis, structural and thermal kinetic analysis

    Science.gov (United States)

    Goel, Nidhi

    2016-08-01

    A new complex [UO2(2,6-DNP)2phen] (1) (2,6-DNP = 2,6-dinitrophenol, phen = 1,10-phenanthroline) was synthesized, and identified by elemental analysis, IR, Powder XRD and single crystal X-ray crystallography. Crystal structure provides the abundant information's about the bonding and geometry around the U(VI) metal center. The thermal decomposition was studied by TG-DSC, and the kinetics of thermolysis was investigated by applying model fitting as well as isoconversional methods. Explosion delay measurement (De) was also evaluated to determine the response of this complex under the condition of rapid heating.

  8. Distribution of intravenously administered acetylcholinesterase inhibitor and acetylcholinesterase activity in the adrenal gland: 11C-donepezil PET study in the normal rat.

    Directory of Open Access Journals (Sweden)

    Tadashi Watabe

    Full Text Available PURPOSE: Acetylcholinesterase (AChE inhibitors have been used for patients with Alzheimer's disease. However, its pharmacokinetics in non-target organs other than the brain has not been clarified yet. The purpose of this study was to evaluate the relationship between the whole-body distribution of intravenously administered (11C-Donepezil (DNP and the AChE activity in the normal rat, with special focus on the adrenal glands. METHODS: The distribution of (11C-DNP was investigated by PET/CT in 6 normal male Wistar rats (8 weeks old, body weight  = 220 ± 8.9 g. A 30-min dynamic scan was started simultaneously with an intravenous bolus injection of (11C-DNP (45.0 ± 10.7 MBq. The whole-body distribution of the (11C-DNP PET was evaluated based on the Vt (total distribution volume by Logan-plot analysis. A fluorometric assay was performed to quantify the AChE activity in homogenized tissue solutions of the major organs. RESULTS: The PET analysis using Vt showed that the adrenal glands had the 2nd highest level of (11C-DNP in the body (following the liver (13.33 ± 1.08 and 19.43 ± 1.29 ml/cm(3, respectively, indicating that the distribution of (11C-DNP was the highest in the adrenal glands, except for that in the excretory organs. The AChE activity was the third highest in the adrenal glands (following the small intestine and the stomach (24.9 ± 1.6, 83.1 ± 3.0, and 38.5 ± 8.1 mU/mg, respectively, indicating high activity of AChE in the adrenal glands. CONCLUSIONS: We demonstrated the whole-body distribution of (11C-DNP by PET and the AChE activity in the major organs by fluorometric assay in the normal rat. High accumulation of (11C-DNP was observed in the adrenal glands, which suggested the risk of enhanced cholinergic synaptic transmission by the use of AChE inhibitors.

  9. Nose-To-Brain Delivery of PLGA-Diazepam Nanoparticles.

    Science.gov (United States)

    Sharma, Deepak; Sharma, Rakesh Kumar; Sharma, Navneet; Gabrani, Reema; Sharma, Sanjeev K; Ali, Javed; Dang, Shweta

    2015-10-01

    The objective of the present investigation was to optimize diazepam (Dzp)-loaded poly(lactic-co-glycolic acid) nanoparticles (NP) to achieve delivery in the brain through intranasal administration. Dzp nanoparticles (DNP) were formulated by nanoprecipitation and optimized using Box-Behnken design. The influence of various independent process variables (polymer, surfactant, aqueous to organic (w/o) phase ratio, and drug) on resulting properties of DNP (z-average and drug entrapment) was investigated. Developed DNP showed z-average 148-337 d.nm, polydispersity index 0.04-0.45, drug entrapment 69-92%, and zeta potential in the range of -15 to -29.24 mV. Optimized DNP were further analyzed by differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), ex-vivo drug release, and in-vitro cytotoxicity. Ex-vivo drug release study via sheep nasal mucosa from DNP showed a controlled release of 64.4% for 24 h. 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay performed on Vero cell line showed less toxicity for DNP as compared to Dzp suspension (DS). Gamma scintigraphy and biodistribution study of DNP and DS was performed on Sprague-Dawley rats using technetium-99m-labeled ((99m)Tc) Dzp formulations to investigate the nose-to-brain drug delivery pathway. Brain/blood uptake ratios, drug targeting efficiency, and direct nose-to-brain transport were found to be 1.23-1.45, 258, and 61% for (99m)Tc-DNP (i.n) compared to (99m)Tc-DS (i.n) (0.38-1.06, 125, and 1%). Scintigraphy images showed uptake of Dzp from nose-to-brain, and this observation was in agreement with the biodistribution results. These results suggest that the developed poly(D,L-lactide-co-glycolide) (PLGA) NP could serve as a potential carrier of Dzp for nose-to-brain delivery in outpatient management of status epilepticus.

  10. Optical Detection of Nuclear Spin States

    Science.gov (United States)

    2007-01-31

    60 mW Gunn diode source (Millitech). DNP and dipolar diffusion In the DNP process the initial enhancement is local to the site of the...consists of 2 Impatt diode sources, both injection locked with a Gunn in order to reduce the phase noise, whose outputs are combined. The data seems...time, and as a function of microwave power. We used a 100 mW Gunn source, and a 1 W custom–built source from Quinstar Technologies. The 1 W source

  11. Distribution of intravenously administered acetylcholinesterase inhibitor and acetylcholinesterase activity in the adrenal gland: 11C-donepezil PET study in the normal rat.

    Science.gov (United States)

    Watabe, Tadashi; Naka, Sadahiro; Ikeda, Hayato; Horitsugi, Genki; Kanai, Yasukazu; Isohashi, Kayako; Ishibashi, Mana; Kato, Hiroki; Shimosegawa, Eku; Watabe, Hiroshi; Hatazawa, Jun

    2014-01-01

    Acetylcholinesterase (AChE) inhibitors have been used for patients with Alzheimer's disease. However, its pharmacokinetics in non-target organs other than the brain has not been clarified yet. The purpose of this study was to evaluate the relationship between the whole-body distribution of intravenously administered (11)C-Donepezil (DNP) and the AChE activity in the normal rat, with special focus on the adrenal glands. The distribution of (11)C-DNP was investigated by PET/CT in 6 normal male Wistar rats (8 weeks old, body weight  = 220 ± 8.9 g). A 30-min dynamic scan was started simultaneously with an intravenous bolus injection of (11)C-DNP (45.0 ± 10.7 MBq). The whole-body distribution of the (11)C-DNP PET was evaluated based on the Vt (total distribution volume) by Logan-plot analysis. A fluorometric assay was performed to quantify the AChE activity in homogenized tissue solutions of the major organs. The PET analysis using Vt showed that the adrenal glands had the 2nd highest level of (11)C-DNP in the body (following the liver) (13.33 ± 1.08 and 19.43 ± 1.29 ml/cm(3), respectively), indicating that the distribution of (11)C-DNP was the highest in the adrenal glands, except for that in the excretory organs. The AChE activity was the third highest in the adrenal glands (following the small intestine and the stomach) (24.9 ± 1.6, 83.1 ± 3.0, and 38.5 ± 8.1 mU/mg, respectively), indicating high activity of AChE in the adrenal glands. We demonstrated the whole-body distribution of (11)C-DNP by PET and the AChE activity in the major organs by fluorometric assay in the normal rat. High accumulation of (11)C-DNP was observed in the adrenal glands, which suggested the risk of enhanced cholinergic synaptic transmission by the use of AChE inhibitors.

  12. Increased axonal regeneration and swellings in intraepidermal nerve fibers characterize painful phenotypes of diabetic neuropathy

    OpenAIRE

    Cheng, H.T.; Dauch, J.R.; Porzio, M.T.; Yanik, B.M.; Hsieh, W; Smith, A.G.; Singleton, J.R.; Feldman, E.L.

    2013-01-01

    We examined changes in intraepidermal nerve fibers (IENFs) to differentiate patients with diabetic neuropathy (DN) and neuropathic pain (DN-P) from those with DN without pain (DN-NOP). Punch skin biopsies were collected from the proximal thigh (PT) and distal leg (DL) of normal subjects (NS), patients with type 2 diabetes without evidence of DN (DM), or DN-P and DN-NOP patients. Protein gene product 9.5 (PGP) immunohistochemistry was used to quantify total IENF, and growth associated protein ...

  13. Strategic Planning and Doctor Of Nursing Practice Education: Developing Today's and Tomorrow's Leaders.

    Science.gov (United States)

    Falk, Nancy L; Garrison, Kenneth F; Brown, Mary-Michael; Pintz, Christine; Bocchino, Joseph

    2015-01-01

    Strategic planning and thinking skills are essential for today's nurse leaders. Doctor of nursing practice (DNP) programs provide an opportunity for developing effective nurse strategists. A well-designed strategy course can stimulate intellectual growth at all levels of Bloom's Taxonomy. Discussion forums in online education provide new opportunities for rich interaction among peers en route to development of well-informed strategic plans. An interprofessional perspective adds a rich and vital aspect to doctoral nursing education and it serves to inform strategic plan development. A roadmap for teaching strategic planning to current and future nursing leaders will guide the integration of essential content into DNP programs.

  14. Atmosphere characterization for simulation of the two optimal wireless terahertz digital communication links.

    Science.gov (United States)

    Mandehgar, Mahboubeh; Yang, Yihong; Grischkowsky, D

    2013-09-01

    Our studies of terahertz pulse propagation in the atmosphere have identified the two most optimal communication channels. The potential of these channels is demonstrated by physically accurate linear dispersion theory calculations of digital pulse propagation, showing it is possible to have two high-performance, point-to-point digital terahertz links in the atmosphere: a direct 95 GHz, 20 km ground link at 9.5 Gb/s with power loss of 10 dB due to water vapor at RH 58% (10 g/m3) and 20°C, and a direct 250 GHz, geosynchronous satellite link at 20.8 Gb/s with a 2 km zenith path with water vapor loss of 9 dB.

  15. WSPEC: A Waveguide Filter Bank Spectrometer

    CERN Document Server

    Che, George; Underhill, Matthew; Mauskopf, Philip; Groppi, Christopher; Jones, Glenn; Johnson, Bradley; McCarrick, Heather; Flanigan, Daniel; Day, Peter

    2015-01-01

    We have designed, fabricated, and measured a 5-channel prototype spectrometer pixel operating in the WR10 band to demonstrate a novel moderate-resolution (R=f/{\\Delta}f~100), multi-pixel, broadband, spectrometer concept for mm and submm-wave astronomy. Our design implements a transmission line filter bank using waveguide resonant cavities as a series of narrow-band filters, each coupled to an aluminum kinetic inductance detector (KID). This technology has the potential to perform the next generation of spectroscopic observations needed to drastically improve our understanding of the epoch of reionization (EoR), star formation, and large-scale structure of the universe. We present our design concept, results from measurements on our prototype device, and the latest progress on our efforts to develop a 4-pixel demonstrator instrument operating in the 130-250 GHz band.

  16. Assessment of 28 nm UTBB FD-SOI technology platform for RF applications: Figures of merit and effect of parasitic elements

    Science.gov (United States)

    Kazemi Esfeh, B.; Kilchytska, V.; Barral, V.; Planes, N.; Haond, M.; Flandre, D.; Raskin, J.-P.

    2016-03-01

    This work provides a detailed study of 28 nm fully-depleted silicon-on-insulator (FD-SOI) planar ultra-thin body and BOX (UTBB) MOSFETs for high frequency applications. All parasitic elements such as the parasitic gate and source/drain series resistances, total capacitances are extracted and their effects on RF performance are analyzed and compared with previous work on similar devices. Two main RF figures of merit (FoM) such as the current gain cut-off frequency (fT) and the maximum oscillation frequency (fmax) are determined. It is shown that fT of ∼280 GHz and fmax of ∼250 GHz are achievable in the shortest devices. Based on the extracted parameters, the validation of the small-signal equivalent circuit used for modeling UTBB MOSFETs is investigated by comparing simulated and measured S-parameters.

  17. Performance of passive terahertz imaging system

    Science.gov (United States)

    Wang, Jia; Zhao, Guozhong

    2016-11-01

    Terahertz (THz) radiation has the higher penetration to clothing, cardboard boxes, plastic packaging materials and other similar dielectrics. Its lower photon energy compared with X-rays make the detected material and the human being to be not destroyed. THz application in field of security are developed by many countries. In this research, we present a multiband of passive terahertz imaging by the thermal radiation measurement. The Noise Equivalent Temperature Difference(NETD) is obtained. The result shows that NETD of the passive imaging system is 0.8K at 94 GHz, and 1.5K at 250GHz. We found that the main source of noise is the noise from detection circuit. Finally, the improvement methods of detecting sensitivity are analyzed and discussed.

  18. Design Analysis of An Electromagnetic Band Gap Microstrip Antenna

    Directory of Open Access Journals (Sweden)

    M. S. Alam

    2011-01-01

    Full Text Available Problem statement: Wideband compact antenna is highly demandable due to the dynamic development in the wireless technology. Approach: A simple, compact EBG microstrip antenna is proposed in this study that covers a wideband of 250 GHz and the design is conformal with the 2.45 GHz ISM band (WLAN, IEEE 802.11b and g/Bluetooth/RFID applications. Results: A 6×6 array of square unit cell formed the EBG structure which is incorporated with the radiating patch to enhance the antenna performances. This design achieved an impedance bandwidth of 10.14% (2.34-2.59 GHz at -10 dB return loss and VSWR ≤ 2. Simulated radiation pattern is almost omnideirectional. Conclusion/Recommendations: The simulated results prove the compatibility of the EBG antenna with the 2.45 GHz ISM band applications. Further enhancement of the antenna performance with improved design is under consideration.

  19. Observation of harmonic gyro-backward-wave oscillation in a 100 GHz CARM oscillator experiment

    Science.gov (United States)

    McCowan, Robert B.; Sullivan, Carol A.; Gold, Steven H.; Fliflet, Arne W.

    1991-02-01

    A cyclotron autoresonance maser (CARM) oscillator experiment is reported, using a 600 keV, 200 A electron beam, and a whispering gallery-mode rippled-wall Bragg cavity. This device was designed to produce tens of megawatts of radiation at 100 GHz from a CARM interaction, but instead has produced only moderate powers (tens of kWs) in fundamental gyrotron modes near 35 GHz, in third-harmonic-gyro-BWO modes, and possible third-harmonic gyrotron modes at frequencies near the expected CARM frequency, with no discernable CARM radiation. The lack of observable CARM radiation is attributed to excessive ripple on the voltage waveform and to mode competition. Calculations of the spectrum and growth rate of the backward-wave oscillations are consistent with the experimental observation.

  20. Development of the fast arcing protection for ECRH system on the HL-2A tokamak

    Institute of Scientific and Technical Information of China (English)

    LI Xiu-juan; RAO Jun; WANG Ming-wei; WANG Xue-yun; LI Bo

    2007-01-01

    The fast arcing protection of the electron cyclotron resonance heating(ECRH)is very important in the heating experiment of the HL-2A tokamak.ECRH has the ability of 1MW using two gyrotrons (each about 500kW).The states of the protected objects must been detect then deal with effectively arcing-events in order to accomplish the protection function.The fast arcing protection includes two parts such as some arcing detectors and one arcing protector for the one gyrotron and one waveguide line.The ECRH arcing protection system must be fast enough and very reliable,and the total response time is less than 25μs.

  1. Steady-State Fully Noninductive Current Driven by Electron Cyclotron Waves in a Magnetically Confined Plasma

    Science.gov (United States)

    Sauter, O.; Henderson, M. A.; Hofmann, F.; Goodman, T.; Alberti, S.; Angioni, C.; Appert, K.; Behn, R.; Blanchard, P.; Bosshard, P.; Chavan, R.; Coda, S.; Duval, B. P.; Fasel, D.; Favre, A.; Furno, I.; Gorgerat, P.; Hogge, J.-P.; Isoz, P.-F.; Joye, B.; Lavanchy, P.; Lister, J. B.; Llobet, X.; Magnin, J.-C.; Mandrin, P.; Manini, A.; Marlétaz, B.; Marmillod, P.; Martin, Y.; Mayor, J.-M.; Martynov, A. A.; Mlynar, J.; Moret, J.-M.; Nieswand, C.; Nikkola, P.; Paris, P.; Perez, A.; Pietrzyk, Z. A.; Pitts, R. A.; Pochelon, A.; Pochon, G.; Refke, A.; Reimerdes, H.; Rommers, J.; Scavino, E.; Tonetti, G.; Tran, M. Q.; Troyon, F.; Weisen, H.

    2000-04-01

    A steady-state, fully noninductive plasma current has been sustained for the first time in a tokamak using electron cyclotron current drive only. In this discharge, 123 kA of current have been sustained for the entire gyrotron pulse duration of 2 s. Careful distribution across the plasma minor radius of the power deposited from three 0.5-MW gyrotrons was essential for reaching steady-state conditions. With central current drive, up to 153 kA of current have been fully replaced transiently for 100 ms. The noninductive scenario is confirmed by the ability to recharge the Ohmic transformer. The dependence of the current drive efficiency on the minor radius is also demonstrated.

  2. Multistage depressed collector conceptual design for thin magnetically confined electron beams

    Science.gov (United States)

    Pagonakis, Ioannis Gr.; Wu, Chuanren; Illy, Stefan; Jelonnek, John

    2016-04-01

    The requirement of higher efficiency in high power microwave devices, such as traveling wave tubes and gyrotrons, guides scientific research to more advanced types of collector systems. First, a conceptual design approach of a multistage depressed collector for a sheet electron beam confined by a magnetic field is presented. The sorting of the electron trajectories, according to their initial kinetic energy, is based on the E × B drift concept. The optimization of the geometrical parameters is based on the analytical equations under several general assumptions. The analysis predicts very high levels of efficiency. Then, a design approach for the application of this type of collector to a gyrotron cylindrical hollow electron beam is also presented with very high levels of efficiency more than 80%.

  3. Propagation of gamma rays and production of free electrons in air

    CERN Document Server

    Dimant, Y S; Sprangle, P; Penano, J; Romero-Talamas, C A; Granatstein, V L

    2012-01-01

    A new concept of remote detection of concealed radioactive materials has been recently proposed \\cite{Gr.Nusin.2010}-\\cite{NusinSprangle}. It is based on the breakdown in air at the focal point of a high-power beam of electromagnetic waves produced by a THz gyrotron. To initiate the avalanche breakdown, seed free electrons should be present in this focal region during the electromagnetic pulse. This paper is devoted to the analysis of production of free electrons by gamma rays leaking from radioactive materials. Within a hundred meters from the radiation source, the fluctuating free electrons appear with the rate that may exceed significantly the natural background ionization rate. During the gyrotron pulse of about 10 microsecond length, such electrons may seed the electric breakdown and create sufficiently dense plasma at the focal region to be detected as an unambiguous effect of the concealed radioactive material.

  4. Development of a Propagating Millimeter-Wave Beam Position and Profile Monitor in the Oversize Corrugated Waveguide Used in an ECRH System

    Science.gov (United States)

    Shimozuma, Takashi; Kobayashi, Sakuji; Ito, Satoshi; Ito, Yasuhiko; Kubo, Shin; Yoshimura, Yasuo; Nishiura, Masaki; Igami, Hiroe; Takahashi, Hiromi; Mizuno, Yoshinori; Okada, Kohta; Mutoh, Takashi

    2016-01-01

    In a high-power electron cyclotron resonance heating (ECRH) system for plasma heating, a long-distance and low-loss transmission system of the millimeter wave is required. A real-time monitor of the millimeter-wave beam position and its intensity profile, which can be used in a high-power, evacuated, and cooled transmission line, is proposed, designed, manufactured, and tested. The beam-position and profile monitor (BPM) consists of a reflector, Peltier-device array, and a heat-sink, which is installed in the reflector-plate of a miterbend. The BPM was tested using both simulated electric heater power and high-power gyrotron output power. The profile obtained from the monitor using the gyrotron output was well agreed with the burn patter on a thermal sensitive paper. Methods of data analysis and mode-content analysis of a propagating millimeter-wave in the corrugated waveguide are proposed.

  5. High power testing of water-cooled waveguide for ITER-like ECH transmission lines

    Science.gov (United States)

    Anderson, J. P.; Doane, J. L.; Grunloh, H. J.; O'Neill, R. C.; Ikeda, R.; Oda, Y.; Takahashi, K.; Sakamoto, K.

    2017-05-01

    The results of high power testing of new water-cooled ECH waveguide components for ITER are presented. The components are a precision-coupled 4.2 m waveguide assembly, a short expansion joint, and water-cooled waveguide for gyrotron commissioning. The testing was conducted at the QST Naka Fusion Institute using gyrotron pulses of 450 kW at 170 GHz for 300 s. Analysis shows that the power absorbed per unit length for the various waveguide components are dependent on location in the transmission line with respect to high order mode generators, such as miter bends. Additionally, larger-than-expected reflections from the load led to high absorption levels in the transmission line.

  6. Research and Development of 2-frequency (110/138 GHz FADIS for JT-60SA ECHCD system

    Directory of Open Access Journals (Sweden)

    Idei H.

    2015-01-01

    Full Text Available A FAst DIrectional Switch (FADIS of 2-frequency (2-ƒ gyrotron system for the JT-60SA project is being developed under collaboration between Japan Atomic Energy Agency (JAEA and Kyushu University. At first, the frequency drift and dip in the gyrotron operation were measured to consider which kind of FADIS is preferred for application in the Electron Cyclotron Heating and Current Drive (ECHCD system for the JT- 60SA. Various types of the FADIS have been considered. A square corrugated waveguide diplexer system with double resonant rings was considered as one of the most attractive FADIS systems for stable high-power and long-pulse operations in the 2-ƒ JT-60SA ECHCD system.

  7. High-Power Microwave Transmission and Mode Conversion Program

    Energy Technology Data Exchange (ETDEWEB)

    Vernon, Ronald J. [Univ. of Wisconsin, Madison, WI (United States)

    2015-08-14

    This is a final technical report for a long term project to develop improved designs and design tools for the microwave hardware and components associated with the DOE Plasma Fusion Program. We have developed basic theory, software, fabrication techniques, and low-power measurement techniques for the design of microwave hardware associated gyrotrons, microwave mode converters and high-power microwave transmission lines. Specifically, in this report we discuss our work on designing quasi-optical mode converters for single and multiple frequencies, a new method for the analysis of perturbed-wall waveguide mode converters, perturbed-wall launcher design for TE0n mode gyrotrons, quasi-optical traveling-wave resonator design for high-power testing of microwave components, and possible improvements to the HSX microwave transmission line.

  8. Fusion development and technology. Technical progress report, October 15, 1990--October 14, 1993

    Energy Technology Data Exchange (ETDEWEB)

    Montgomery, D.B.

    1992-06-01

    This report discusses the following: superconducting magnet technology; high field superconductors; advanced magnetic system and divertor development; poloidal field coils; gyrotron development; commercial reactor studies--aries; ITER physics: alpha physics and alcator R&D for ITER; lower hybrid current drive and heating in the ITER device; ITER superconducting PF scenario and magnet analysis; ITER systems studies; and safety, environmental and economic factors in fusion development.

  9. Fusion development and technology

    Energy Technology Data Exchange (ETDEWEB)

    Montgomery, D.B.

    1992-01-01

    This report discusses the following: superconducting magnet technology; high field superconductors; advanced magnetic system and divertor development; poloidal field coils; gyrotron development; commercial reactor studies--aries; ITER physics: alpha physics and alcator R D for ITER; lower hybrid current drive and heating in the ITER device; ITER superconducting PF scenario and magnet analysis; ITER systems studies; and safety, environmental and economic factors in fusion development.

  10. Study of Mode Coupling on Coaxial Resonators

    Institute of Scientific and Technical Information of China (English)

    Rui Liu; Hong-Fu Li

    2011-01-01

    A study of mode coupling phenomenon of coaxial resonators has been conducted with theories.Through establishing the source-free transmission line equation,boundary conditions of the coaxial resonators with a corrugated inner conductor are analyzed.In the end,calculations are performed in a wide range of corrugation parameters for the resonator of the Karisruhe Institute of Technology (KIT) relevant coaxial gyrotron.

  11. Program status 3. quarter -- FY 1994: ITER and technology

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-07-19

    During this quarter all technical work and documentation of the PULSAR design was completed. They also assisted UCLA in the planning of the DEMO program.In the area of RF technology, a decision was made to fabricate 4in x 4in gyrotron distributed window. An finally, they obtained good agreement between code predictions and measured data for the up and down field redeposition of tungsten in the DIMES-8 experiment.

  12. Preliminary conceptual design of DEMO EC system

    Energy Technology Data Exchange (ETDEWEB)

    Garavaglia, S., E-mail: garavaglia@ifp.cnr.it; Bin, W.; Bruschi, A.; Granucci, G.; Moro, A.; Rispoli, N. [Institute of Plasma Physics “P.Caldirola”, National Research Council of Italy, Milan (Italy); Grossetti, G.; Strauss, D. [IAM-AWP, Kaiserstr. 12, D-76131 Karlsruhe (Germany); Jelonnek, J. [IHM, KIT, Kaiserstr. 12, D-76131 Karlsruhe (Germany); Tran, Q. M. [CRPP, EPFL, EURATOM Association, CH-1015 Lausanne (Switzerland); Franke, T. [EUROfusion Consortium, Boltzmannstr. 2, D-85748 Garching (Germany); Max-Planck-Institut für Plasmaphysik, Garching (Germany)

    2015-12-10

    In the framework of EUROfusion Consortium the Work Package Heating and Current Drive addresses the engineering design and R&D for the electron cyclotron, ion cyclotron and neutral beam systems. This paper reports the activities performed in 2014, focusing on the work done regarding the input for the conceptual design of the EC system, particularly for the gyrotron, the transmission line and the launchers.

  13. ¿Llegó el tiempo de la agricultura?

    Directory of Open Access Journals (Sweden)

    Machado C. Absalón

    1995-06-01

    Full Text Available El objetivo de este breve comentario es analizar los postuladosque se formulan en el Plan de Desarrollo El Salto Social, [Presidencia-DNP 1994] de modo que no se consideran otros documentos oficiales expedidos antes o después de la publicación del Plan.

  14. Seasonal population density and winter survival strategies of endangered Kashmir gray langur (Semnopithecus ajax) in Dachigam National Park, Kashmir, India.

    Science.gov (United States)

    Mir, Zaffar Rais; Noor, Athar; Habib, Bilal; Veeraswami, Gopi Govindan

    2015-01-01

    The population density of Kashmir gray langurs (Semnopithecus ajax) was studied in Dachigam National Park (DNP), Kashmir using distance sampling method. A total of 13 transects (1.5-2.5 km in length) were surveyed in the intensive study area (~90 km(2)) yielding 170 encounters in different seasons of the study period (2011-2013). Some aspects of behavior and feeding were also studied during the winter months (Dec-Feb) of 2012 and 2013 inside DNP. We used instantaneous scan sampling to collect behavioral data determining the time budget and diet of langurs in winter conditions. Results suggested that the density of Kashmir gray langurs varied marginally across seasons, with the highest density recorded during winter and lowest during summer season. Langurs spent most of their time in carrying out various social activities (34.32 %) and least in resting (18.41 %). Langurs fed upon 13 plant species (belonging to 12 families) and consumed a substantial proportion of bark (37.4 %) in their diet. We conclude that langur density is low in DNP as compared to other plain areas of the Indian subcontinent and langurs in DNP have balanced their time budget and diet so as to increase their chances of survival in the unfavorably cold and food scarce winter conditions.

  15. Theory of coherent dynamic nuclear polarization in quantum dots

    DEFF Research Database (Denmark)

    Neder, Izhar; Rudner, Mark Spencer; Halperin, Bertrand

    2014-01-01

    We consider the production of dynamic nuclear spin polarization (DNP) in a two-electron double quantum dot, in which the electronic levels are repeatedly swept through a singlet-triplet avoided crossing. Our analysis helps to elucidate the intriguing interplay between electron-nuclear hyperfine...

  16. Doctor of Professional Counseling: The Next Step

    Science.gov (United States)

    Southern, Stephen; Cade, Rochelle; Locke, Don W.

    2012-01-01

    Professional doctorates have been established in the allied health professions by clinicians seeking the highest levels of independent practice. Allied health professional doctorates include nursing practice (DNP), occupational therapy (OTD), psychology (PsyD), social work (DSW), and marriage and family therapy (DMFT). Lessons learned from the…

  17. Degradation of 2,4-dinitroanisole (DNAN) by metabolic cooperative activity of Pseudomonas sp. strain FK357and Rhodococcus imtechensis strain RKJ300.

    Science.gov (United States)

    Khan, Fazlurrahman; Pal, Deepika; Ghosh, Anuradha; Cameotra, Swaranjit Singh

    2013-11-01

    2,4-Dinitroanisole (DNAN) is an insensitive explosive ingredient used by many defense agencies as a replacement for 2,4,6-trinitrotoluene. Although the biotransformation of DNAN under anaerobic condition has been reported, aerobic microbial degradation pathway has not been elucidated. An n-methyl-4-nitroaniline degrading bacterium Pseudomonas sp. strain FK357 transformed DNAN into 2,4-dinitrophenol (2,4-DNP) as an end product. Interestingly, when strain FK357 was co-cultured with a 2,4-DNP degrading Rhodococcus imtechensis strain RKJ300, complete and high rate of DNAN degradation was observed with no accumulation of intermediates. Enzyme assay using cell extracts of strain FK357 demonstrated that O-demethylation reaction is the first step of DNAN degradation with formation of 2,4-DNP and formaldehyde as intermediates. Subsequently, 2,4-DNP was degraded by strain RKJ300 via the formation of hydride-Meisenheimer complex. The present study clearly demonstrates that complete degradation of DNAN occurs as a result of the metabolic cooperative activity of two members within a bacterial consortium.

  18. Investigating tumor perfusion by hyperpolarized (13) C MRI with comparison to conventional gadolinium contrast-enhanced MRI and pathology in orthotopic human GBM xenografts

    DEFF Research Database (Denmark)

    Park, Ilwoo; von Morze, Cornelius; Lupo, Janine M

    2016-01-01

    Dissolution dynamic nuclear polarization (DNP) enables the acquisition of (13) C magnetic resonance data with a high sensitivity. Recently, metabolically inactive hyperpolarized (13) C-labeled compounds have shown to be potentially useful for perfusion imaging. The purpose of this study was to va...

  19. Self-assessment of nursing informatics competencies for doctor of nursing practice students.

    Science.gov (United States)

    Choi, Jeungok; Zucker, Donna M

    2013-01-01

    This study examined the informatics competencies of doctor of nursing practice (DNP) students and whether these competencies differed between DNP students in the post-baccalaureate (BS) and post-master's (MS) tracks. Self-reported informatics competencies were collected from 132 DNP students (68 post-BS and 64 post-MS students) in their first year in the program (2007 to 2010). Students were assessed in 18 areas of 3 competency categories: computer skills, informatics knowledge, and informatics skills. Post-BS students were competent in 4 areas (computer skills in communication, systems, documentation, and informatics knowledge about impact of information management), whereas post-MS students were competent in only 1 area (computer skills in communication). Students in both tracks reported computer skills in decision support as their least competent area. Overall, post-BS students reported slightly higher than or similar competency scores as post-MS students, but scores were statistically significant in only 3 of 18 areas. The assessment indicated that knowledge and skills on informatics competencies need to be improved, especially in computer skills for data access and use of decision support systems. Strategies are suggested to integrate competencies into existing informatics course and DNP curricula. Further studies are recommended using an objective measure of informatics competencies.

  20. Resonance-inclined optical nuclear spin polarization of liquids in diamond structures

    Science.gov (United States)

    Chen, Q.; Schwarz, I.; Jelezko, F.; Retzker, A.; Plenio, M. B.

    2016-02-01

    Dynamic nuclear polarization (DNP) of molecules in a solution at room temperature has the potential to revolutionize nuclear magnetic resonance spectroscopy and imaging. The prevalent methods for achieving DNP in solutions are typically most effective in the regime of small interaction correlation times between the electron and nuclear spins, limiting the size of accessible molecules. To solve this limitation, we design a mechanism for DNP in the liquid phase that is applicable for large interaction correlation times. Importantly, while this mechanism makes use of a resonance condition similar to solid-state DNP, the polarization transfer is robust to a relatively large detuning from the resonance due to molecular motion. We combine this scheme with optically polarized nitrogen-vacancy (NV) center spins in nanodiamonds to design a setup that employs optical pumping and is therefore not limited by room temperature electron thermal polarization. We illustrate numerically the effectiveness of the model in a flow cell containing nanodiamonds immobilized in a hydrogel, polarizing flowing water molecules 4700-fold above thermal polarization in a magnetic field of 0.35 T, in volumes detectable by current NMR scanners.

  1. Studies on the Performance of Different Coiled Column Configurations for Compact Type-I Counter-current Chromatography

    Science.gov (United States)

    Yang, Yi; Gu, Dongyu; Aisa, Haji Akber; Ito, Yoichiro

    2011-01-01

    Three types of novel coiled column configurations, i.e., a triangular coiled column and elliptical coiled columns I and II, were designed for type-I countercurrent chromatography and their performances were evaluated with two solvent systems each with suitable test samples. Three DNP-amino acids (DNP-DL-glu, DNP-β-ala and DNP-L-ala) were separated with a moderately hydrophobic two-phase solvent system composed of hexane-ethyl acetate-metanol-0.1M hydrochloric acid (1:1:1:1, v/v), while two dipeptides (tryptophyl tyrosine and valyl-tyrosine) were separated with a polar solvent system composed of 1-butanol-acetic acid-water (4.75:0.25:5, v/v). The overall results indicated that the performance of compact type-I counter-current chromatography was improved by elliptical coiled column II which was mounted with its maximum coil diameter perpendicular to the surface of the column holder. Hydrodynamic effects involved in these separations were discussed. PMID:21491597

  2. Studies on the performance of different coiled column configurations for compact type-I countercurrent chromatography.

    Science.gov (United States)

    Yang, Yi; Gu, Dongyu; Aisa, Haji Akber; Ito, Yoichiro

    2011-05-01

    Three types of novel coiled column configurations, i.e. a triangular coiled column and elliptical coiled columns I and II, were designed for type-I countercurrent chromatography and their performances were evaluated with two solvent systems each with suitable test samples. Three dinitrophenyl (DNP) amino acids (DNP-DL-glu, DNP-β-ala and DNP-L-ala) were separated with a moderately hydrophobic two-phase solvent system composed of hexane-ethyl acetate-metanol-0.1 M hydrochloric acid (1:1:1:1, v/v), while two dipeptides (tryptophyl-tyrosine and valyl-tyrosine) were separated with a polar solvent system composed of 1-butanol-acetic acid-water (4.75:0.25:5, v/v). The overall results indicated that the performance of compact type-I countercurrent chromatography was improved by elliptical coiled column I which was mounted with its maximum coil diameter perpendicular to the surface of the column holder. Hydrodynamic effects involved in these separations were discussed. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. A novel wireless Love wave biosensor platform for multifunctional detection

    Science.gov (United States)

    Song, Taehyeon; Nam, Minwoo; Song, SeungYeon; Yoon, Hyun C.; Lee, Keekeun

    2011-02-01

    This paper presents a novel wireless Love wave biosensor platform for multi-functional detection. A 440MHz wireless and surface acoustic wave (SAW)-based biosensor was developed on a 41° YX LiNbO3 piezoelectric substrate for the simultaneous detection of Anti- Dinitrophenyl-KLH (anti-DNP) immunoglobulin G (IgG). The developed biosensor was composed of a SAW reflective delay lines structured by an interdigital transducer (IDT), shorted grating reflectors, poly(methyl-methacrylate) (PMMA) layer and two sensitive films (Cr/Au). The PMMA was used for the waveguide layer. Coupling of mode (COM) modeling was conducted to determine the optimal device parameters prior to fabrication. The fabricated devices were wirelessly characterized by using the network analyzer as the reader unit. The binding of anti-DNP to DNP receptor molecules induced a change in phase shifts of the original reflection peaks due to a mass loading effect. The phase shifts increased linearly with increasing anti-DNP concentration. The measured reflective coefficient S11 in the time domain showed high signal/noise (S/N) ratio, small signal attenuation, and few spurious peaks. The time positions of the reflection peaks were well matched with the predicted values from the simulation. The obtained sensitivity was 167.9°/μg/ml and 44.8°/ μg/ml for the 1st and the 2nd sensing area, respectively.

  4. Metabolism of acyclic and cyclic N-nitrosamines in cultured human bronchi

    DEFF Research Database (Denmark)

    1977-01-01

    bronchial specimens, these N-nitrosamines and/or their metabolites bound to bronchial mucosal DNA and protein. Binding levels were higher to protein than to DNA. Binding levels of DNP were as high as those with the two acyclic N-nitrosamines DMN and DEN, but binding levels of NPy and NPd were lower. Human...

  5. Wirelessly Driven and Battery-Free Love Wave Biosensor Based on Dinitrophenyl Immobilization

    Science.gov (United States)

    Oh, Haekwan; Wang, Wen; Lee, Keekeun; Yoon, Hyun C.; Yang, Sangsik

    2009-06-01

    A Love wave-based wireless immunosensor with a 440 MHz operating frequency was developed on a 41° YX LiNbO3 piezoelectric substrate. The developed sensor was composed of a shear horizontal surface acoustic wave (SH SAW) reflective delay line, a poly(methyl methacrylate) (PMMA) waveguide layer, and a 2,4-dinitrophenyl (DNP) receptor layer that specifically responds to an anti-DNP immunoglobulin G (IgG). Fabricated devices were wirelessly tested using a network analyzer. Four reflection peaks with large signal/noise (S/N) ratios and sharp reflection peaks were observed in the time domain, and are attributed to three shorted grating reflectors and DNP-bound gold film. With 10 mW radio frequency (RF) power from the network analyzer, a readout distance of ˜1 m was observed. The binding of IgG to DNP receptor molecules induced a change in the Love wave velocity owing to a mass loading effect, resulting in phase shifts of the original reflection peaks. The phase shifts increased linearly with increasing IgG concentration. Fast response time and long-term stability of the developed sensor in a liquid environment were observed. The phase shifts depending on different PMMA thicknesses were also investigated to identify the optimal PMMA waveguide thickness.

  6. Simultaneous Acquisition of Multi-nuclei Enhanced NMR/MRI by Solution State Dynamic Nuclear Polarization

    CERN Document Server

    He, Yugui; Feng, Jiwen; Huang, Chongyang; Chen, Fang; Liu, Maili; Liu, Chaoyang

    2015-01-01

    Dynamic nuclear polarization (DNP) has become a very important hyperpolarization method because it can dramatically increase the sensitivity of nuclear magnetic resonance (NMR) of various molecules. Liquid-state DNP based on Overhauser effect is capable of directly enhancing polarizations of all kinds of nuclei in the system. The combination of simultaneous Overhauser multi-nuclei enhancements with the multi-nuclei parallel acquisitions provides a variety of important applications in both MR spectroscopy (MRS) and image (MRI). Here we present two simple illustrative examples for simultaneously enhanced multi-nuclear spectra and images to demonstrate the principle and superiority. We have observed very large simultaneous DNP enhancements for different nuclei, such as 1H and 23Na, 1H and 31P, 19F and 31P, especially for the first time to report sodium ion enhancement in liquid. We have also obtained the simultaneous imaging of 19H and 31P at low field by solution-state DNP for the first time. This method can ob...

  7. Hyperpolarization of nitrogen-15 nuclei by cross polarization and dissolution dynamic nuclear polarization

    Science.gov (United States)

    Milani, Jonas; Vuichoud, Basile; Bornet, Aurélien; Melzi, Roberto; Jannin, Sami; Bodenhausen, Geoffrey

    2017-01-01

    Dynamic Nuclear Polarization (DNP) is often achieved by the direct transfer of polarization from electrons to nuclei such as 13C, induced by microwave saturation of the wings of narrow EPR lines of radicals like trityl. In the indirect approach on the other hand, DNP is used to transfer the polarization from the electrons of radicals such as nitroxides that have broad EPR lines to nuclear spins I = 1H, followed by cross-polarization (CP) from I = 1H to S = 13C or other nuclei with low gyromagnetic ratios. This approach is particularly attractive for S = 15N, since direct DNP yields modest polarizations P(15N) 2 h. In this paper, we show that CP from 1H to 15N at 1.2 K can yield P(15N) = 25% with τCP-DNP(15N) = 10-15 min. After rapid dissolution and transfer to a solution-state NMR spectrometer, a polarization P(15N) = 20% was observed at 300 K. The longitudinal relaxation times in solution can be as long as T1(15N) > 800 s in favorable cases.

  8. Highly sensitive and adaptable fluorescence-quenched pair discloses the substrate specificity profiles in diverse protease families

    Science.gov (United States)

    Poreba, Marcin; Szalek, Aleksandra; Rut, Wioletta; Kasperkiewicz, Paulina; Rutkowska-Wlodarczyk, Izabela; Snipas, Scott J.; Itoh, Yoshifumi; Turk, Dusan; Turk, Boris; Overall, Christopher M.; Kaczmarek, Leszek; Salvesen, Guy S.; Drag, Marcin

    2017-01-01

    Internally quenched fluorescent (IQF) peptide substrates originating from FRET (Förster Resonance Energy Transfer) are powerful tool for examining the activity and specificity of proteases, and a variety of donor/acceptor pairs are extensively used to design individual substrates and combinatorial libraries. We developed a highly sensitive and adaptable donor/acceptor pair that can be used to investigate the substrate specificity of cysteine proteases, serine proteases and metalloproteinases. This novel pair comprises 7-amino-4-carbamoylmethylcoumarin (ACC) as the fluorophore and 2,4-dinitrophenyl-lysine (Lys(DNP)) as the quencher. Using caspase-3, caspase-7, caspase-8, neutrophil elastase, legumain, and two matrix metalloproteinases (MMP2 and MMP9), we demonstrated that substrates containing ACC/Lys(DNP) exhibit 7 to 10 times higher sensitivity than conventional 7-methoxy-coumarin-4-yl acetic acid (MCA)/Lys(DNP) substrates; thus, substantially lower amounts of substrate and enzyme can be used for each assay. We therefore propose that the ACC/Lys(DNP) pair can be considered a novel and sensitive scaffold for designing substrates for any group of endopeptidases. We further demonstrate that IQF substrates containing unnatural amino acids can be used to investigate protease activities/specificities for peptides containing post-translationally modified amino acids. Finally, we used IQF substrates to re-investigate the P1-Asp characteristic of caspases, thus demonstrating that some human caspases can also hydrolyze substrates after glutamic acid. PMID:28230157

  9. Clinical value of ipsi- and contralateral sacral reflex latency measurement: a normative data study in man.

    Science.gov (United States)

    Amarenco, G; Kerdraon, J

    2000-01-01

    The latency of the bulbocavernosus reflex (BCR) evoked by electrical stimulation of the penis provides a measure of the conduction velocity over the sacral reflex arc at the S2-4 level but does not allow evaluation of the side affected since it results from the simultaneous excitation of both dorsal nerves of the penis (DNP) at the penile root. To evaluate the reliability of the side-to-side BCR latency measurement, this study compared the reflex characteristics of the response elicited by both DNP stimulation and unilateral DNP block. After a unilateral selective DNP anesthesic block, we found that the early response of the contralateral BCR is strictly ipsilateral with no differences in terms of latency, morphology, and reflex threshold from controls. This result may indicate that the side-to-side BCR latency measurement allows a comparative study of the respective right and left sacral reflex arcs in men. We found a mean inter-latency difference of 1.8 +/- 0.4 millisecond of the early BCR response after simultaneous recording of the right and left sides in 10 normal men. We established that an inter-latency difference >3 milliseconds may be indicative of a significant alteration in the conduction over the sacral reflex arc.

  10. Theory of coherent dynamic nuclear polarization in quantum dots

    DEFF Research Database (Denmark)

    Neder, Izhar; Rudner, Mark Spencer; Halperin, Bertrand

    2014-01-01

    coupling, electronic spin-orbit coupling, and electron and nuclear Larmor precession in an externally- applied magnetic field, in guiding the production of DNP. In particular, we specifically address the roles of multiple nuclear spin species. By treating the nuclear spin dynamics semi-classically, we...

  11. Investigating the Tolerance of Tomato (Lycopersicon esculentum Cultivars to Broomrape (Orobanche aegyptiaca pers in Khorassan Razavi

    Directory of Open Access Journals (Sweden)

    M. Zafarian,

    2014-03-01

    Full Text Available To investigate the tolerance of tomato cultivars to Egyptian broomrape (Orobanche aegyptiaca pers., an experiment was conducted in a randomized complete block design with 11 treatments and 3 replications in mazrae Nemoune Astan Quds Razavi in Mashhad, Iran, 2012. Treatment were 11 varieties (Peto early CH, Sterling (Karon, Khorram, Petorak, DNP 3005, PS 6515, SPEEDY, IDEN, VADI STAR, FIRINZEH and DNP 3001 which were transplanted in the field along with broomrape. Sampling was done at two stages: 1- after appearance and establishment of broomrape on tomato root where the dry weight, stem number and node numbers of broomrape on tomato root and tomato dry weight were measured and 2- at the end of growing season where tomato fruit weight and its yield were determined. Result indicated that Sterling and Khorram cultivars did have the least broomrape dry weight, stem number and node numbers of broomrape on tomato root and while produced highest plant dry weight, fruit and yield as compared to the other cultivars. Thus, may be considered as tolerant cultivars. Petorak and DNP 3001 on the other hand, presented the most broomrape dry weight, stem number and node number on tomato root. However, Petorak, Peto early CH and FIRINZEH cultivars produced the least plant weight, fruit and yield and thus, they can be called the sensitive cultivars. DNP 3001 being highly attacked by broomrape produced increased fruit yield and therefore compensated its ill effects.

  12. Mentoring Nurses in Political Skill to Navigate Organizational Politics

    Science.gov (United States)

    2016-01-01

    Objective. The aim of this study was to describe and analyze the correlations between mentoring functions and political skill development among nurses who have earned or are candidates for a Ph.D. or doctorate of nursing practice (DNP) degree. Background. The healthcare system is in flux; future generations of Ph.D. and DNP nurse leaders will be required to demonstrate political acumen. Political skill to navigate organizational politics has had limited research within nursing. Methods. A cross-sectional research design using a web-based survey of 222 nurses who have earned or are candidates for a Ph.D. or DNP. This study utilized two validated tools to measure mentoring functions and political skill. Results. The response rate was 52% (n = 115) of which 86 were Ph.D. and 29 were DNPs. An informal mentoring relationship was described by 62% of the respondents and formal mentoring by 35% of the protégés; only 25% (n = 74) established a mentoring contract. Mentoring score showed significance for total political skill and moderate effect on the networking ability. The mentoring functions of advocacy, career development facilitation, learning facilitation, and friendship were found to correlate significantly with total political skill scores. Conclusions. This study established a benefit for nurses who have earned or are candidates for a Ph.D. or DNP from mentoring to support political skill development. PMID:27777798

  13. Overhauser effects in insulating solids

    NARCIS (Netherlands)

    Can, T. V.; Caporini, M. A.; Mentink-Vigier, F.; Corzilius, B.; Walish, J. J.; Rosay, M.; Maas, W. E.; Baldus, M.; Vega, S.; Swager, T. M.; Griffin, R. G.

    2014-01-01

    We report magic angle spinning, dynamic nuclear polarization (DNP) experiments at magnetic fields of 9.4 T, 14.1 T, and 18.8 T using the narrow line polarizing agents 1,3-bisdiphenylene-2-phenylallyl (BDPA) dispersed in polystyrene, and sulfonated-BDPA (SA-BDPA) and trityl OX063 in glassy glycerol/w

  14. Chitosan aerogel: a recyclable, heterogeneous organocatalyst for the asymmetric direct aldol reaction in water.

    Science.gov (United States)

    Ricci, Alfredo; Bernardi, Luca; Gioia, Claudio; Vierucci, Simone; Robitzer, Mike; Quignard, Françoise

    2010-09-14

    Aerogel microspheres of chitosan, an abundant biopolymer obtained from marine crustaceans, have been successfully applied to catalyze the asymmetric aldol reaction in water, providing the products in high yields and with good stereoselectivity (up to 93% ee) and recyclability (up to 4 runs). Yields were favourably affected by additives such as DNP and stearic acid.

  15. Electroanalytical determination of donepezil HCl in tablets and human serum by differential pulse and osteryoung square wave voltammetry at a glassy carbon electrode.

    Science.gov (United States)

    Golcu, A; Ozkan, S A

    2006-09-01

    Donepezil hydrochloride (DNP) is used for the treatment of mild to moderate dementia of the Alzheimer's type. The voltammetric behavior of DNP was studied at a glassy carbon electrode using cyclic, linear sweep, differential pulse (DPV) and square-wave (OSWV) voltammetric techniques. DNP exhibited irreversible anodic waves within the pH range 1.80 and 9.00 in different supporting electrolytes. The peak was characterized as being irreversible and diffusion-controlled. The possible mechanism of the oxidation process is discussed. The current-concentration plot was rectilinear over the range from 1 x 10(-6) to 1 x 10(-4) M in Britton-Robinson buffer at pH 7.0 with a correlation coefficient between 0.997 and 0.999 in supporting electrolyte and human serum samples using the DPV and SWV techniques. The repeatability and reproducibility of the methods for both media (supporting electrolyte and serum sample) were determined. Precision and accuracy of the developed methods were demonstrated by recovery studies. The standard addition method was used for the recovery studies. No electroactive interferences were found in biological fluids from endogenous substances or additives present in tablets. The methods developed were successfully applied to the determination of DNP in tablets and in spiked human serum.

  16. 76 FR 38206 - Notice of Application for Disclaimer of Interest; Pennington County, South Dakota

    Science.gov (United States)

    2011-06-29

    ... Montana State Office, 5001 Southgate Drive, Billings, MT 59101-4669. Only written comments will be... Office, 5001 Southgate Drive, Billings, MT 59101-4669; 406-896-5053. Persons who use a telecommunications... Resources. BILLING CODE 4310-DN-P...

  17. 75 FR 3489 - Notice of Public Meeting, Eastern Montana Resource Advisory Council Meeting

    Science.gov (United States)

    2010-01-21

    ... Montana Resource Advisory Council will be held on March 4, 2010, in Billings, MT. The meeting will start... in Montana. At these meetings, topics will include: Miles City and Billings Field Office manager..., 2010. M. Elaine Raper, District Manager. BILLING CODE 4310-DN-P...

  18. Mitochondrial ATP is required for the maintenance of membrane integrity in stallion spermatozoa, whereas motility requires both glycolysis and oxidative phosphorylation

    NARCIS (Netherlands)

    Davila, M Plaza; Muñoz, P Martin; Bolaños, J M Gallardo; Stout, T A E; Gadella, B M; Tapia, J A; da Silva, C Balao; Ferrusola, C Ortega; Peña, F J

    2016-01-01

    To investigate the hypothesis that oxidative phosphorylation is a major source of ATP to fuel stallion sperm motility, oxidative phosphorylation was suppressed using the mitochondrial uncouplers CCCP and 2,4,-dinitrophenol (DNP) and by inhibiting mitochondrial respiration at complex IV using sodium

  19. On The Potential of Dynamic Nuclear Polarization Enhanced Diamonds in Solid-State and Dissolution (13) C NMR Spectroscopy.

    Science.gov (United States)

    Bretschneider, Christian O; Akbey, Ümit; Aussenac, Fabien; Olsen, Greg L; Feintuch, Akiva; Oschkinat, Hartmut; Frydman, Lucio

    2016-09-01

    Dynamic nuclear polarization (DNP) is a versatile option to improve the sensitivity of NMR and MRI. This versatility has elicited interest for overcoming potential limitations of these techniques, including the achievement of solid-state polarization enhancement at ambient conditions, and the maximization of (13) C signal lifetimes for performing in vivo MRI scans. This study explores whether diamond's (13) C behavior in nano- and micro-particles could be used to achieve these ends. The characteristics of diamond's DNP enhancement were analyzed for different magnetic fields, grain sizes, and sample environments ranging from cryogenic to ambient temperatures, in both solution and solid-state experiments. It was found that (13) C NMR signals could be boosted by orders of magnitude in either low- or room-temperature solid-state DNP experiments by utilizing naturally occurring paramagnetic P1 substitutional nitrogen defects. We attribute this behavior to the unusually long electronic/nuclear spin-lattice relaxation times characteristic of diamond, coupled with a time-independent cross-effect-like polarization transfer mechanism facilitated by a matching of the nitrogen-related hyperfine coupling and the (13) C Zeeman splitting. The efficiency of this solid-state polarization process, however, is harder to exploit in dissolution DNP-enhanced MRI contexts. The prospects for utilizing polarized diamond approaching nanoscale dimensions for both solid and solution applications are briefly discussed.

  20. Exchange interaction-driven dynamic nuclear polarization in Mn-doped InGaAs/GaAs quantum dots

    Science.gov (United States)

    Krebs, O.; Baudin, E.; Lemaître, A.

    2016-11-01

    We investigated optical spin orientation and dynamic nuclear polarization (DNP) in individual self-assembled InGaAs/GaAs quantum dots (QDs) doped by a single Mn atom, a magnetic impurity providing a neutral acceptor A0 with an effective spin J =1 . We find that the spin of an electron photocreated in such a quantum dot can be efficiently oriented by a quasiresonant circularly polarized excitation. For the electron spin levels which are made quasidegenerate by a magnetic field compensating the exchange interaction Δe with A0, there is however a full depolarization due the anisotropic part of the exchange. Still, in most studied QDs, the spin polarized photoelectrons give rise to a pronounced DNP which grows with a longitudinal magnetic field until a critical field where it abruptly vanishes. For some QDs, several replica of such DNP sequence are observed at different magnetic fields. This striking behavior is qualitatively discussed as a consequence of different exchange interactions experienced by the electron, driving the DNP rate via the energy cost of electron-nucleus spin flip-flops.

  1. Imagining the Past

    DEFF Research Database (Denmark)

    Kinzel, Moritz; Tanaka, Mina

    2016-01-01

    experience and to support the dissemination of results of the ongoing excavations. Besides traditional techniques, the Qatar Islamic Archaeology and Heritage Project in cooperation with DNP have developed two mobile applications with AR-elements and interactive components to engage especially young visitors...

  2. Milli-tesla NMR and spectrophotometry of liquids hyperpolarized by dissolution dynamic nuclear polarization

    Science.gov (United States)

    Zhu, Yue; Chen, Chia-Hsiu; Wilson, Zechariah; Savukov, Igor; Hilty, Christian

    2016-09-01

    Hyperpolarization methods offer a unique means of improving low signal strength obtained in low-field NMR. Here, simultaneous measurements of NMR at a field of 0.7 mT and laser optical absorption from samples hyperpolarized by dissolution dynamic nuclear polarization (D-DNP) are reported. The NMR measurement field closely corresponds to a typical field encountered during sample injection in a D-DNP experiment. The optical spectroscopy allows determination of the concentration of the free radical required for DNP. Correlation of radical concentration to NMR measurement of spin polarization and spin-lattice relaxation time allows determination of relaxivity and can be used for optimization of the D-DNP process. Further, the observation of the nuclear Overhauser effect originating from hyperpolarized spins is demonstrated. Signals from 1H and 19F in a mixture of trifluoroethanol and water are detected in a single spectrum, while different atoms of the same type are distinguished by J-coupling patterns. The resulting signal changes of individual peaks are indicative of molecular contact, suggesting a new application area of hyperpolarized low-field NMR for the determination of intermolecular interactions.

  3. Embryotoxicity of nitrophenols to the early life stages of zebrafish (Danio rerio).

    Science.gov (United States)

    Ceylan, Zeynep; Şişman, Turgay; Yazıcı, Zehra; Altıkat, Aysun Özen

    2016-08-01

    The nitrophenols (NPs) are water-soluble compounds. These compounds pose a significant health threat since they are priority environmental pollutants. In this study, 2-Nitrophenol (2NP) and 2,4-dinitrophenol (DNP) were examined for embryo and early life stage toxicity in zebrafish (Danio rerio). Acute toxicity and teratogenicity of 2NP and DNP were tested for 4 days using zebrafish embryos. The typical lesions observed were no somite formation, incomplete eye and head development, tail curvature, weak pigmentation (≤48 hours postfertilization (hpf)), kyphosis, scoliosis, yolk sac deformity, and nonpigmentation (72 hpf). Also, embryo and larval mortality increased and hatching success decreased. The severity of abnormalities and mortalities were concentration- and compound-dependent. Of the compounds tested, 2,4-DNP was found to be highly toxic to the fish embryos following exposure. The median lethal concentrations and median effective concentrations for 2NP are 18.7 mg/L and 7.9 mg/L, respectively; the corresponding values for DNP are 9.65 mg/L and 3.05 mg/L for 48 h. The chorda deformity was the most sensitive endpoint measured. It is suggested that the embryotoxicity may be mediated by an oxidative phosphorylation uncoupling mechanism. This article is the first to describe the teratogenicity and embryotoxicity of two NPs to the early life stages of zebrafish.

  4. 78 FR 67368 - Agency Information Collection Activities: Submission to OMB for Review and Approval; Public...

    Science.gov (United States)

    2013-11-12

    ....11 217.7 Degree and Post Nursing Master's Certificate Student To Receive Traineeship Support by Role.../or DNP) Degree Nursing Students To Receive Traineeship Support by Role (budget year 1 and budget year... Education Nursing Traineeship (AENT) Program Application. OMB No.: 0915-xxxx--NEW Abstract: The...

  5. Waveguide transition with vacuum window for multiband dynamic nuclear polarization systems

    DEFF Research Database (Denmark)

    Rybalko, Oleksandr; Bowen, Sean; Zhurbenko, Vitaliy

    2016-01-01

    A low loss waveguide transition section and oversized microwave vacuum window covering several frequency bands (94 GHz, 140 GHz, 188 GHz) is presented. The transition is compact and was optimized for multiband Dynamic Nuclear Polarization (DNP) systems in a full-wave simulator. The window is more...

  6. Tunable 13C/1H dual channel matching circuit for dynamic nuclear polarization system with cross-polarization

    DEFF Research Database (Denmark)

    Rybalko, Oleksandr; Bowen, Sean; Zhurbenko, Vitaliy

    2016-01-01

    In this paper we report initial results of design and practical implementation of tuning and matching circuit to estimate a performance of Dynamic Nuclear Polarization (DNP) at a magnetic field of 6.7 T. It is shown that developed circuit for signal observation is compact, easy to make and provides...

  7. Cluster formation restricts dynamic nuclear polarization of xenon in solid mixtures

    DEFF Research Database (Denmark)

    Kuzma, N. N.; Pourfathi, M.; Kara, H.

    2012-01-01

    During dynamic nuclear polarization (DNP) at 1.5 K and 5 T, Xe-129 nuclear magnetic resonance (NMR) spectra of a homogeneous xenon/1-propanol/trityl-radical solid mixture exhibit a single peak, broadened by H-1 neighbors. A second peak appears upon annealing for several hours at 125 K. Its...

  8. Recycling and imaging of nuclear singlet hyperpolarization

    DEFF Research Database (Denmark)

    Pileio, Giuseppe; Bowen, Sean; Laustsen, Christoffer

    2013-01-01

    observation of the same batch of polarized nuclei over a period of 30 min and more. We report a recycling protocol in which the enhanced nuclear polarization achieved by dissolution-DNP is observed with full intensity and then returned to singlet order. MRI experiments may be run on a portion of the available...

  9. Electron paramagnetic resonance and dynamic nuclear polarization of char suspensions: surface science and oximetry

    DEFF Research Database (Denmark)

    Clarkson, R B; Odintsov, B M; Ceroke, P J

    1998-01-01

    ; they can be calibrated and used for oximetry. Biological stability and low toxicity make chars good sensors for in vivo measurements. Scalar and dipolar interactions of water protons at the surfaces of chars may be utilized to produce dynamic nuclear polarization (DNP) of the nuclear spin population...

  10. Oxidative effect of several intravenous iron complexes in the rat.

    Science.gov (United States)

    Bailie, George R; Schuler, Catherine; Leggett, Robert E; Li, Hsin; Li, Hsin-Dat; Patadia, Hiten; Levin, Robert

    2013-06-01

    The objective of this study was to compare the oxidative stress induced in rat internal organs by the administration of the following clinically used intravenous (IV) iron (Fe) containing compounds: iron sucrose (IS), iron dextran (ID), ferric carboxymaltose and ferumoxytol. Groups of six adult rats received 1 mg/kg of each compound weekly for 5 doses. Seven days following the last dose, animals were euthanized and tissue samples of heart, lung, liver, and kidney were obtained, washed in warmed saline and frozen under liquid nitrogen and stored at -80 °C for analysis for nitrotyrosine (NT) and dinitro phenyl (DNP) as markers of oxidative stress. All tissues showed a similar pattern of oxidative stress. All Fe products stimulated an increase in the tissue concentration of both NT and DNP. In general, DNP was stimulated significantly less than NT except for IS. DNP was stimulated to an equal degree except for ID where NT was significantly higher than the NT concentrations in all other Fe compounds. ID produced over 10-fold the concentration of NT than any other Fe. IV Fe compounds present a risk of oxidative stress to a variety of internal organs. However, we found that IS was the least damaging and ID was the worst.

  11. Delta-cyclodextrin as novel chiral probe for enantiomeric separation by electromigration methods

    DEFF Research Database (Denmark)

    Wistuba, Dorothee; Bogdanski, Anja; Larsen, Kim Lambertsen

    2006-01-01

    Native d-CD has been employed as chiral selector in CE and MEKC. To investigate the potential of the enantiodiscriminating properties of d-CD, negatively charged 5-dimethylamino-1-naphthalene-sulfonyl (dansyl)-, 2,4-dinitrophenyl (DNP)- and FMOC-derivatives of several amino acids, 1,1’-binaphthyl...

  12. Poly(L-histidine) based copolymers: Effect of the chemically substituted L-histidine on the physio-chemical properties of the micelles and in vivo biodistribution.

    Science.gov (United States)

    Zhang, Xiaojun; Chen, Dawei; Ba, Shuang; Chang, Jing; Zhou, Jiaying; Zhao, Haixia; Zhu, Jia; Zhao, Xiuli; Hu, Haiyang; Qiao, Mingxi

    2016-04-01

    Even though the Poly(l-histidine) (PHis) based copolymers have been well studied, the effect of the chemically substituted l-histidine on the physio-chemical and biological properties of the micelles has never been elucidated to date. To address this issue, triblock copolymer of poly(ethylene glycol)-poly(D,L-lactide)-poly(2,4-dinitrophenol-L-histidine)(mPEG-b-PLA-b-DNP-PHis) with DNP group substituted to the saturated nitrogen of l-histidine were synthesized. The pH sensitive properties of the copolymer micelles were characterized using an acid-base titration method, fluorescene probe technique, DLS observation, in vitro drug release and cytotoxicity against MCF-7 cells under different pH conditions, respectively. The results suggest that mPEG-b-PLA-b-DNP-PHis copolymers showed similar micellar stability for DOX loaded micelles, increased particle size, and similar pH responsive properties with mPEG-b-PLA-b-PHis copolymers. The subcellular distribution observation demonstrated that mPEG-b-PLA-b-DNP-PHis micelles showed a slightly compromised endo-lysosmal escape of doxorubicin as compared to mPEG-b-PLA-b-PHis micelles. The mPEG-b-PLA-b-DNP-PHis micelles showed higher cellular uptake by MCF-7 cells than mPEG-b-PLA-b-PHis micelles due to the different uptake pathways. Effect of DNP substitution on the in vivo distribution of the copolymer micelles was studied using non-invasive near-infrared fluorescence (NIRF) imaging with mPEG-b-PLA-b-PHis micelles as control. The results indicate that the mPEG-b-PLA-b-DNP-PHis micelles showed a reduced passive targeting to the tumor due to the larger particle size. These results suggest that saturated nitrogen of PHis may serve as a valuable site for chemical modification of the PHis based copolymers because of the little effect on the pH responsive properties. However, selection of the substitution group needs to be considered due to the possible increase of micellar particle size of the micelles, leading to compromised passive

  13. Inhibition of oxidative phosphorylation for enhancing citric acid production by Aspergillus niger.

    Science.gov (United States)

    Wang, Lu; Zhang, Jianhua; Cao, Zhanglei; Wang, Yajun; Gao, Qiang; Zhang, Jian; Wang, Depei

    2015-01-16

    The spore germination rate and growth characteristics were compared between the citric acid high-yield strain Aspergillus niger CGMCC 5751 and A. niger ATCC 1015 in media containing antimycin A or DNP. We inferred that differences in citric acid yield might be due to differences in energy metabolism between these strains. To explore the impact of energy metabolism on citric acid production, the changes in intracellular ATP, NADH and NADH/NAD+ were measured at various fermentation stages. In addition, the effects of antimycin A or DNP on energy metabolism and citric acid production was investigated by CGMCC 5751. By comparing the spore germination rate and the extent of growth on PDA plates containing antimycin A or DNP, CGMCC 5751 was shown to be more sensitive to antimycin A than ATCC 1015. The substrate-level phosphorylation of CGMCC 5751 was greater than that of ATCC 1015 on PDA plates with DNP. DNP at tested concentrations had no apparent effect on the growth of CGMCC 5751. There were no apparent effects on the mycelial morphology, the growth of mycelial pellets or the dry cell mass when 0.2 mg L(-1) antimycin A or 0.1 mg L(-1) DNP was added to medium at the 24-h time point. The concentrations of intracellular ATP, NADH and NADH/NAD+ of CGMCC 5751 were notably lower than those of ATCC 1015 at several fermentation stages. Moreover, at 96 h of fermentation, the citric acid production of CGMCC 5751 reached up to 151.67 g L(-1) and 135.78 g L(-1) by adding 0.2 mg L(-1) antimycin A or 0.1 mg L(-1) DNP, respectively, at the 24-h time point of fermentation. Thus, the citric acid production of CGMCC 5751 was increased by 19.89% and 7.32%, respectively. The concentrations of intracellular ATP, NADH and NADH/NAD+ of the citric acid high-yield strain CGMCC 5751 were notably lower than those of ATCC 1015. The excessive ATP has a strong inhibitory effect on citric acid accumulation by A. niger. Increasing NADH oxidation and appropriately reducing the concentration of

  14. Operational performance and plans for the ECH system on DIII-D

    Science.gov (United States)

    Lohr, J.; Cengher, M.; Gorelov, Y. A.; Ponce, D.; Torrezan, A.; Ives, L.; Read, M.; Leviness, A.

    2016-10-01

    The ECH system on the DIII-D tokamak currently comprises 6 gyrotrons operating at 110 GHz and injecting 3.5 MW for administratively limited pulse lengths up to 5 sec. A 7th gyrotron generating 1.5 MW at 117.5 GHz is planned for installation late in 2016. Production of this tube was delayed due to issues related to reflected electrons resulting in internal arcs during initial testing. Performance reliability of the individual gyrotrons in the DIII-D complex has exceeded 90% for a wide variety of operational modes, including fast modulation and rapid poloidal sweeping of the rf beams using high speed dc motors and magnetic position encoders. Measures have been taken to reduce the risk of damage to launcher hardware and diagnostics from inadvertent operation of the tokamak at densities such that the right hand cutoff frequency is present in the plasma. The system has occasionally been used in non-fusion applications. The most recent of these has been testing a CVD diamond waveguide blocking window at the Brewster angle. Work supported by the US DOE under DE-FC02-04ER54698 and DE-AC02-09CH11466.

  15. NTM stabilization by alternating O-point EC current drive using a high-power diplexer

    Science.gov (United States)

    Kasparek, W.; Doelman, N.; Stober, J.; Maraschek, M.; Zohm, H.; Monaco, F.; Eixenberger, H.; Klop, W.; Wagner, D.; Schubert, M.; Schütz, H.; Grünwald, G.; Plaum, B.; Munk, R.; Schlüter, K. H.; ASDEX Upgrade Team

    2016-12-01

    At the tokamak ASDEX Upgrade, experiments to stabilize neoclassical tearing modes (NTMs) by electron cyclotron (EC) heating and current drive in the O-points of the magnetic islands were performed. For the first time, injection into the O-points of the revolving islands was performed via a fast directional switch, which toggled the EC power between two launchers synchronously to the island rotation. The switching was performed by a resonant diplexer employing a sharp resonance in the transfer function, and a small frequency modulation of the feeding gyrotron around the slope of the resonance. Thus, toggling of the power between the two outputs of the diplexer connected to two articulating launchers was possible. Phasing and control of the modulation were performed via a set of Mirnov coils and appropriate signal processing. In the paper, technological issues, the design of the diplexer, the tracking of the diplexer resonance to the gyrotron frequency, the generation and processing of control signals for the gyrotron, and the typical performance concerning switching contrast and efficiency are discussed. The plasma scenario is described, and plasma experiments are presented, where the launchers scanned the region of the resonant surface continuously and also where the launchers were at a fixed position near to the q  =  1.5-surface. In the second case, complete stabilization of a 3/2 NTM could be reached. These experiments are also seen as a technical demonstration for the applicability of diplexers in large-scale ECRH systems.

  16. Self-consistent modeling of terahertz waveguide and cavity with frequency-dependent conductivity

    Science.gov (United States)

    Huang, Y. J.; Chu, K. R.; Thumm, M.

    2015-01-01

    The surface resistance of metals, and hence the Ohmic dissipation per unit area, scales with the square root of the frequency of an incident electromagnetic wave. As is well recognized, this can lead to excessive wall losses at terahertz (THz) frequencies. On the other hand, high-frequency oscillatory motion of conduction electrons tends to mitigate the collisional damping. As a result, the classical theory predicts that metals behave more like a transparent medium at frequencies above the ultraviolet. Such a behavior difference is inherent in the AC conductivity, a frequency-dependent complex quantity commonly used to treat electromagnetics of metals at optical frequencies. The THz region falls in the gap between microwave and optical frequencies. However, metals are still commonly modeled by the DC conductivity in currently active vacuum electronics research aimed at the development of high-power THz sources (notably the gyrotron), although a small reduction of the DC conductivity due to surface roughness is sometimes included. In this study, we present a self-consistent modeling of the gyrotron interaction structures (a metallic waveguide or cavity) with the AC conductivity. The resulting waveguide attenuation constants and cavity quality factors are compared with those of the DC-conductivity model. The reduction in Ohmic losses under the AC-conductivity model is shown to be increasingly significant as the frequency reaches deeper into the THz region. Such effects are of considerable importance to THz gyrotrons for which the minimization of Ohmic losses constitutes a major design consideration.

  17. TFTR 60 GHz alpha particle collective Thomson Scattering diagnostic

    Energy Technology Data Exchange (ETDEWEB)

    Machuzak, J.S.; Woskov, P.P.; Gilmore, J. [Massachusetts Inst. of Tech., Cambridge, MA (United States); Bretz, N.L.; Park, H.K. [Princeton Univ., NJ (United States). Plasma Physics Lab.; Aamodt, R.E.; Cheung, P.Y.; Russell, D.A. [Lodestar Research Corp., Boulder, CO (United States); Bindslev, H. [JET Joint Undertaking, Abingdon (United Kingdom)

    1995-03-01

    A 60 GHz gyrotron collective Thomson Scattering alpha particle diagnostic has been implemented for the D-T period on TFM. Gyrotron power of 0.1-1 kW in pulses of up to 1 second can be launched in X-mode. Efficient corrugated waveguides are used with antennaes and vacuum windows of the TFTR Microwave Scattering system. A multichannel synchronous detector receiver system and spectrum analyzer acquire the scattered signals. A 200 Megasample/sec digitizer is used to resolve fine structure in the frequency spectrum. By scattering nearly perpendicular to the magnetic field, this experiment will take advantage of an enhancement of the scattered signal which results from the interaction of the alpha particles with plasma resonances in the lower hybrid frequency range. Significant enhancements are expected, which will make these measurements possible with gyrotron power less than 1 kW, while maintaining an acceptable signal to noise ratio. We hope to extract alpha particle density and velocity distribution functions from the data. The D and T fuel densities and temperatures may also be obtainable by measurement of the respective ion cyclotron harmonic frequencies.

  18. Experimental Verification on Remote Detectability of Concealed Radioactive Material Based on the Plasma Discharge Delay Time using High-Power Millimeter-Wave

    Science.gov (United States)

    Kim, Dongsung; Yu, Dongho; Sawant, Ashwini; Choe, Mun Seok; Lee, Ingeun; Choi, Eunmi

    2016-10-01

    We experimentally demonstrate a remote detection method of a radioactive source by plasma breakdown using high-power millimeter-wave source, gyrotron. A number of free electrons near the radioactive source are much higher than those of without the radioactive source (roughly 10 particles/cm3) owing to the interaction of air molecules and strong gamma rays generated by radioactive material. The RF wave beam is focused in ambient air, and the plasmas discharge occurs involving random delay time which means a time interval between the RF wave and a fluorescent light caused by the plasma. We observed that the delay time decreased significantly due to the high density of free electrons in Ar plasma with an existence of Co60 radioactive material. This technique of delay time measurement shows 1000 times more sensitive than a method of detectable mass equation to identify the existence of radioactive source remotely. It is the first experimental verification of radioactive material detection using a high power gyrotron. This study shows that a remote detection of radioactive material based on analysis of precise delay time measurement could be feasible by using a high power millimeter/THz wave gyrotron. NRF-2013R1A1A2061062, NRF-2012-Global Ph.D. Fellowship Program.

  19. Recent progress of high-power millimeter wavelength gyrodevices

    Science.gov (United States)

    Goldenberg, A. L.; Litvak, A. G.

    1995-06-01

    This presentation reviews recent progress of Russian physicists in developing electronic devices of the gyroresonance type, which are so far the most advanced sources of millimeter microwaves. It deals with the concept of the gyrotron as a device operating at higher volume resonator modes with a built-in quasioptical converter of the output radiation into a wave beam. That concept made it possible to create comparatively simple and reliable long-pulse and quasicontinuous devices with a power level of 0.5 MW in the frequency band 30-160 GHz. Analysis of the principal problems of projects for continuous-wave (CW) gyrotrons of near 1 MW output power (choice of an operating mode, requirements to an electron beam, peculiarities of built-in converters) is included, as well as the results of model experiments. For amplifying millimeter wavelength gyroklystrons, a level of several hundreds of kW at the frequency 35 GHz, and up to 65 kW at frequency 94 GHz were also achieved. Some modifications of gyrotrons for material processing and special research are described.

  20. Construction and (13) C hyperpolarization efficiency of a 180 GHz dissolution dynamic nuclear polarization system.

    Science.gov (United States)

    Kiswandhi, Andhika; Niedbalski, Peter; Parish, Christopher; Ferguson, Sarah; Taylor, David; McDonald, George; Lumata, Lloyd

    2017-09-01

    Dynamic nuclear polarization (DNP) via the dissolution method has become one of the rapidly emerging techniques to alleviate the low signal sensitivity in nuclear magnetic resonance (NMR) spectroscopy and imaging. In this paper, we report on the development and (13) C hyperpolarization efficiency of a homebuilt DNP system operating at 6.423 T and 1.4 K. The DNP hyperpolarizer system was assembled on a wide-bore superconducting magnet, equipped with a standard continuous-flow cryostat, and a 180 GHz microwave source with 120 mW power output and wide 4 GHz frequency tuning range. At 6.423 T and 1.4 K, solid-state (13) C polarization P levels of 64% and 31% were achieved for 3 M [1-(13) C] sodium acetate samples in 1 : 1 v/v glycerol:water glassing matrix doped with 15 mM trityl OX063 and 40 mM 4-oxo-TEMPO, respectively. Upon dissolution, which takes about 15 s to complete, liquid-state (13) C NMR signal enhancements as high as 240 000-fold (P=21%) were recorded in a nearby high resolution (13) C NMR spectrometer at 1 T and 297 K. Considering the relatively lower cost of our homebuilt DNP system and the relative simplicity of its design, the dissolution DNP setup reported here could be feasibly adapted for in vitro or in vivo hyperpolarized (13) C NMR or magnetic resonance imaging at least in the pre-clinical setting. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

  1. A profile of U.S. nursing faculty in research- and practice-focused doctoral education.

    Science.gov (United States)

    Smeltzer, Suzanne C; Sharts-Hopko, Nancy C; Cantrell, Mary Ann; Heverly, Mary Ann; Nthenge, Serah; Jenkinson, Amanda

    2015-03-01

    This study, which is part of a larger project, was conducted to profile the nursing faculty in the United States teaching in PhD and DNP programs. This is a descriptive study. A sample of 554 nursing faculty who teach in PhD and DNP programs was recruited by email solicitation to represent all geographic regions of the United States. Data were collected from November 2013 through January 2014 using an online survey instrument. The instrument was developed based on results of review of the literature and of focus groups of doctoral faculty (faculty teaching in doctoral programs) to ascertain characteristics of faculty teaching in doctoral programs and of the schools in which they teach. Frequencies and descriptive statistics are reported. Growth in DNP programs has outpaced growth in PhD programs, and DNP graduates have moved into doctoral education in greater numbers than PhD graduates. DNP faculty report less prior experience and current productivity scholarship than faculty in PhD programs only or both types of programs. Strategies are needed to ensure that doctoral programs are staffed by faculty who are prepared for doctoral education and the development of nursing science. The Institute of Medicine has recommended doubling the number of doctorally prepared nurses in the United States by 2020 to ensure that sufficient numbers of faculty are available to prepare the nursing labor force that is needed for delivery of healthcare services. Nurse scientists are needed to contribute to improvement in patient care quality and safety, and practice leaders are needed to facilitate the translation of research into safe, high-quality, and cost-effective care. The landscape of doctoral education in nursing is rapidly changing. © 2015 Sigma Theta Tau International.

  2. Programmed Cell Death Induced by (−)-8,9-Dehydroneopeltolide in Human Promyelocytic Leukemia HL-60 Cells under Energy Stress Conditions

    Science.gov (United States)

    Fuwa, Haruhiko; Sato, Mizuho; Sasaki, Makoto

    2014-01-01

    (+)-Neopeltolide is a marine macrolide natural product that exhibits potent antiproliferative activity against several human cancer cell lines. Previous study has established that this natural product primarily targets the complex III of the mitochondrial electron transport chain. However, the biochemical mode-of-actions of neopeltolide have not been investigated in detail. Here we report that (−)-8,9-dehydroneopeltolide (8,9-DNP), a more accessible synthetic analogue, shows potent cytotoxicity against human promyelocytic leukemia HL-60 cells preferentially under energy stress conditions. Nuclear morphology analysis, as well as DNA ladder assay, indicated that 8,9-DNP induced significant nuclear condensation/fragmentation and DNA fragmentation, and these events could be suppressed by preincubating the cells with a pan-caspase inhibitor, N-benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone (zVAD). Immunoblot analysis demonstrated the release of cytochrome c from the mitochondria and the cleavage of full-length caspase-3 and poly(ADP-ribose) polymerase (PARP). These results indicated that 8,9-DNP induced caspase-dependent apoptotic programmed cell death under energy stress conditions. It was also found that 8,9-DNP induced non-apoptotic cell death in the presence/absence of zVAD under energy stress conditions. Immunoblot analysis showed the intracytosolic release of apoptosis-inducing factor (AIF), although it did not further translocate to the nucleus. It appears most likely that, in the presence of zVAD, 8,9-DNP triggered necrotic cell death as a result of severe intracellular ATP depletion. PMID:25419998

  3. Programmed Cell Death Induced by (−-8,9-Dehydroneopeltolide in Human Promyelocytic Leukemia HL-60 Cells under Energy Stress Conditions

    Directory of Open Access Journals (Sweden)

    Haruhiko Fuwa

    2014-11-01

    Full Text Available (+-Neopeltolide is a marine macrolide natural product that exhibits potent antiproliferative activity against several human cancer cell lines. Previous study has established that this natural product primarily targets the complex III of the mitochondrial electron transport chain. However, the biochemical mode-of-actions of neopeltolide have not been investigated in detail. Here we report that (−-8,9-dehydroneopeltolide (8,9-DNP, a more accessible synthetic analogue, shows potent cytotoxicity against human promyelocytic leukemia HL-60 cells preferentially under energy stress conditions. Nuclear morphology analysis, as well as DNA ladder assay, indicated that 8,9-DNP induced significant nuclear condensation/fragmentation and DNA fragmentation, and these events could be suppressed by preincubating the cells with a pan-caspase inhibitor, N-benzyloxycarbonyl-Val-Ala-Asp(OMe-fluoromethylketone (zVAD. Immunoblot analysis demonstrated the release of cytochrome c from the mitochondria and the cleavage of full-length caspase-3 and poly(ADP-ribose polymerase (PARP. These results indicated that 8,9-DNP induced caspase-dependent apoptotic programmed cell death under energy stress conditions. It was also found that 8,9-DNP induced non-apoptotic cell death in the presence/absence of zVAD under energy stress conditions. Immunoblot analysis showed the intracytosolic release of apoptosis-inducing factor (AIF, although it did not further translocate to the nucleus. It appears most likely that, in the presence of zVAD, 8,9-DNP triggered necrotic cell death as a result of severe intracellular ATP depletion.

  4. A continuous fluorescent assay for the determination of plasma and tissue angiotensin I-converting enzyme activity

    Directory of Open Access Journals (Sweden)

    M.F. Alves

    2005-06-01

    Full Text Available A continuous assay using internally quenched fluorescent peptides with the general sequence Abz-peptidyl-(DnpP-OH (Abz = ortho-aminobenzoic acid; Dnp = 2,4-dinitrophenyl was optimized for the measurement of angiotensin I-converting enzyme (ACE in human plasma and rat tissues. Abz-FRK(DnpP-OH, which was cleaved at the Arg-Lys bond by ACE, was used for the enzyme evaluation in human plasma. Enzymatic activity was monitored by continuous recording of the fluorescence (lambdaex = 320 nm and lambdaem = 420 nm at 37ºC, in 0.1 M Tris-HCl buffer, pH 7.0, with 50 mM NaCl and 10 µM ZnCl2. The assays can be performed directly in the cuvette of the fluorimeter and the hydrolysis followed for 5 to 10 min. ACE measurements in the plasma of 80 healthy patients with Hip-His-Leu and with Abz-FRK(DnpP-OH correlated closely (r = 0.90, P < 0.001. The specificity of the assay was demonstrated by the complete inhibition of hydrolysis by 0.5 µM lisinopril or captopril. Abz-FRK(DnpP-OH cleavage by ACE was monitored in rat lung, kidney, heart, and liver homogenates in the presence of a cocktail of inhibitors containing trans-epoxy-succinyl-L-leucylamido-(4-guanido-butene, pepstatin, phenyl-methylsulfonyl fluoride, N-tosyl-L-phenylalanyl-chloromethyl ketone, and N-tosyl-lysyl-chloromethyl ketone to prevent undesirable hydrolysis. ACE activity in lung, heart and kidney homogenates, but not in liver homogenates, was completely abolished by 0.5 µM lisinopril or captopril. The advantages of the method are the procedural simplicity and the high sensitivity providing a rapid assay for ACE determinations.

  5. Preparation, characterization, and in vivo evaluation of intranasally administered liposomal formulation of donepezil

    Directory of Open Access Journals (Sweden)

    Al Asmari AK

    2016-01-01

    Full Text Available Abdulrahman K Al Asmari,1 Zabih Ullah,1 Mohammad Tariq,1 Amal Fatani21Department of Research, Prince Sultan, Military Medical City, 2Department of Pharmacy, King Saud University, Riyadh, Saudi ArabiaAbstract: The adequate amount of drug delivery to the brain in neurological patients is a major problem faced by the physicians. Recent studies suggested that intranasal administration of liposomal formulation may improve the drug delivery to the brain. In the present study, an attempt was made to study the brain bioavailability of commonly used anti-Alzheimer drug donepezil (DNP liposomal formulation by intranasal route in rats. We adopted the thin layer hydration technique for the preparation of liposomes by using cholesterol, polyethylene glycol, and 1,2-distearyl-sn-glycero-3-phosphocholine (DSPC. The prepared liposomes were characterized by determining particle size, shape, surface morphology, zeta potential, encapsulation efficiency, and in vitro release of DNP. The pharmacokinetic parameters of liposomal DNP in plasma and brain of rats were determined following oral and nasal administration. The results of this study showed that the DNP liposomal formulation was stable with a consistent size (102±3.3 nm and shape. The prepared liposomes showed high encapsulation efficiency (84.91%±3.31% and sustained-release behavior. The bioavailability of DNP in plasma and brain increased significantly (P<0.05 after administration of liposomal formulation by the intranasal route. Histopathological examination showed that the formulation was safe and free from toxicity. It can be concluded that the nasal administration of liposomal preparation may provide an efficient and reliable mode of drug delivery to the central nervous system.Keywords: donepezil, intranasal, liposomes, bioavailability, blood–brain barrier

  6. Overhauser effects in insulating solids

    Science.gov (United States)

    Can, T. V.; Caporini, M. A.; Mentink-Vigier, F.; Corzilius, B.; Walish, J. J.; Rosay, M.; Maas, W. E.; Baldus, M.; Vega, S.; Swager, T. M.; Griffin, R. G.

    2014-08-01

    We report magic angle spinning, dynamic nuclear polarization (DNP) experiments at magnetic fields of 9.4 T, 14.1 T, and 18.8 T using the narrow line polarizing agents 1,3-bisdiphenylene-2-phenylallyl (BDPA) dispersed in polystyrene, and sulfonated-BDPA (SA-BDPA) and trityl OX063 in glassy glycerol/water matrices. The 1H DNP enhancement field profiles of the BDPA radicals exhibit a significant DNP Overhauser effect (OE) as well as a solid effect (SE) despite the fact that these samples are insulating solids. In contrast, trityl exhibits only a SE enhancement. Data suggest that the appearance of the OE is due to rather strong electron-nuclear hyperfine couplings present in BDPA and SA-BDPA, which are absent in trityl and perdeuterated BDPA (d21-BDPA). In addition, and in contrast to other DNP mechanisms such as the solid effect or cross effect, the experimental data suggest that the OE in non-conducting solids scales favorably with magnetic field, increasing in magnitude in going from 5 T, to 9.4 T, to 14.1 T, and to 18.8 T. Simulations using a model two spin system consisting of an electron hyperfine coupled to a 1H reproduce the essential features of the field profiles and indicate that the OE in these samples originates from the zero and double quantum cross relaxation induced by fluctuating hyperfine interactions between the intramolecular delocalized unpaired electrons and their neighboring nuclei, and that the size of these hyperfine couplings is crucial to the magnitude of the enhancements. Microwave power dependent studies show that the OE saturates at considerably lower power levels than the solid effect in the same samples. Our results provide new insights into the mechanism of the Overhauser effect, and also provide a new approach to perform DNP experiments in chemical, biophysical, and physical systems at high magnetic fields.

  7. Overhauser effects in insulating solids

    Energy Technology Data Exchange (ETDEWEB)

    Can, T. V.; Corzilius, B.; Walish, J. J.; Griffin, R. G. [Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States); Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States); Caporini, M. A.; Rosay, M.; Maas, W. E. [Bruker BioSpin, Billerica, Massachusetts 01821 (United States); Mentink-Vigier, F.; Vega, S. [Weizmann Institute of Science, Rehovot (Israel); Baldus, M. [NMR Spectroscopy, Bijvoet Center for Biomolecular Research, Department of Chemistry, Faculty of Science, Utrecht University, 3584 CH Utrecht (Netherlands); Swager, T. M. [Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)

    2014-08-14

    We report magic angle spinning, dynamic nuclear polarization (DNP) experiments at magnetic fields of 9.4 T, 14.1 T, and 18.8 T using the narrow line polarizing agents 1,3-bisdiphenylene-2-phenylallyl (BDPA) dispersed in polystyrene, and sulfonated-BDPA (SA-BDPA) and trityl OX063 in glassy glycerol/water matrices. The {sup 1}H DNP enhancement field profiles of the BDPA radicals exhibit a significant DNP Overhauser effect (OE) as well as a solid effect (SE) despite the fact that these samples are insulating solids. In contrast, trityl exhibits only a SE enhancement. Data suggest that the appearance of the OE is due to rather strong electron-nuclear hyperfine couplings present in BDPA and SA-BDPA, which are absent in trityl and perdeuterated BDPA (d{sub 21}-BDPA). In addition, and in contrast to other DNP mechanisms such as the solid effect or cross effect, the experimental data suggest that the OE in non-conducting solids scales favorably with magnetic field, increasing in magnitude in going from 5 T, to 9.4 T, to 14.1 T, and to 18.8 T. Simulations using a model two spin system consisting of an electron hyperfine coupled to a {sup 1}H reproduce the essential features of the field profiles and indicate that the OE in these samples originates from the zero and double quantum cross relaxation induced by fluctuating hyperfine interactions between the intramolecular delocalized unpaired electrons and their neighboring nuclei, and that the size of these hyperfine couplings is crucial to the magnitude of the enhancements. Microwave power dependent studies show that the OE saturates at considerably lower power levels than the solid effect in the same samples. Our results provide new insights into the mechanism of the Overhauser effect, and also provide a new approach to perform DNP experiments in chemical, biophysical, and physical systems at high magnetic fields.

  8. Novel design for centrifugal counter-current chromatography: VI. Ellipsoid column.

    Science.gov (United States)

    Gu, Dongyu; Yang, Yi; Xin, Xuelei; Aisa, Haji Akber; Ito, Yoichiro

    2015-01-01

    A novel ellipsoid column was designed for centrifugal counter-current chromatography. Performance of the ellipsoid column with a capacity of 3.4 mL was examined with three different solvent systems composed of 1-butanol-acetic acid-water (4:1:5, v/v) (BAW), hexane-ethyl acetate-methanol-0.1 M HCl (1:1:1:1, v/v) (HEMH), and 12.5% (w/w) PEG1000 and 12.5% (w/w) dibasic potassium phosphate in water (PEG-DPP) each with suitable test samples. In dipeptide separation with BAW system, both stationary phase retention (Sf) and peak resolution (Rs) of the ellipsoid column were much higher at 0° column angle (column axis parallel to the centrifugal force) than at 90° column angle (column axis perpendicular to the centrifugal force), where elution with the lower phase at a low flow rate produced the best separation yielding Rs at 2.02 with 27.8% Sf at a flow rate of 0.07 ml/min. In the DNP-amino acid separation with HEMW system, the best results were obtained at a flow rate of 0.05 ml/min with 31.6% Sf yielding high Rs values at 2.16 between DNP-DL-glu and DNP-β-ala peaks and 1.81 between DNP-β-ala and DNP-L-ala peaks. In protein separation with PEG-DPP system, lysozyme and myolobin were resolved at Rs of 1.08 at a flow rate of 0.03 ml/min with 38.9% Sf. Most of those Rs values exceed those obtained from the figure-8 column under similar experimental conditions previously reported.

  9. Following Metabolism in Living Microorganisms by Hyperpolarized (1)H NMR.

    Science.gov (United States)

    Dzien, Piotr; Fages, Anne; Jona, Ghil; Brindle, Kevin M; Schwaiger, Markus; Frydman, Lucio

    2016-09-21

    Dissolution dynamic nuclear polarization (dDNP) is used to enhance the sensitivity of nuclear magnetic resonance (NMR), enabling monitoring of metabolism and specific enzymatic reactions in vivo. dDNP involves rapid sample dissolution and transfer to a spectrometer/scanner for subsequent signal detection. So far, most biologically oriented dDNP studies have relied on hyperpolarizing long-lived nuclear spin species such as (13)C in small molecules. While advantages could also arise from observing hyperpolarized (1)H, short relaxation times limit the utility of prepolarizing this sensitive but fast relaxing nucleus. Recently, it has been reported that (1)H NMR peaks in solution-phase experiments could be hyperpolarized by spontaneous magnetization transfers from bound (13)C nuclei following dDNP. This work demonstrates the potential of this sensitivity-enhancing approach to probe the enzymatic process that could not be suitably resolved by (13)C dDNP MR. Here we measured, in microorganisms, the action of pyruvate decarboxylase (PDC) and pyruvate formate lyase (PFL)-enzymes that catalyze the decarboxylation of pyruvate to form acetaldehyde and formate, respectively. While (13)C NMR did not possess the resolution to distinguish the starting pyruvate precursor from the carbonyl resonances in the resulting products, these processes could be monitored by (1)H NMR at 500 MHz. These observations were possible in both yeast and bacteria in minute-long kinetic measurements where the hyperpolarized (13)C enhanced, via (13)C → (1)H cross-relaxation, the signals of protons binding to the (13)C over the course of enzymatic reactions. In addition to these spontaneous heteronuclear enhancement experiments, single-shot acquisitions based on J-driven (13)C → (1)H polarization transfers were also carried out. These resulted in higher signal enhancements of the (1)H resonances but were not suitable for multishot kinetic studies. The potential of these (1)H-based approaches for

  10. Role of ATP in the sensitivity to heat and the induction of apoptosis in mammalian cells.

    Science.gov (United States)

    Miyazaki, N; Kurihara, K; Nakano, H; Shinohara, K

    2002-01-01

    Heat-induced cell death and apoptosis were studied with respect to intracellular ATP. Studies on the relationship between hyperthermic cell-killing at 44 degrees C and cellular ATP levels in four cell lines grown as monolayers and six cell lines grown in suspension showed good correlations between cellular ATP levels and the sensitivity to heat. D(0) values (the dose required to reduce survival in the linear portion of the response by 63%) linearly increased with an increase in cellular ATP levels. No such changes in sensitivity to heat were observed between the cells cultured at different cell densities, regardless of the change in the cellular ATP level. These results suggest that cellular intrinsic ability to supply ATP rather than the level of pooled ATP per se is responsible for the thermal response. Heat-induced apoptosis in L5178Y cells was observed following treatment at 42 degrees C for 70 min, 44 degrees C for 20 min or 47 degrees C for 3 min, which corresponded to surviving fractions of 25, 0.6 and 0.8%, respectively, but not at 47 degrees C for 20 min, indicating that mild heat shock induced apoptosis. 2-deoxyglucose (2DG) and 2,4-dinitrophenol (DNP) increased the sensitivity to heat and affected the mode of cell death. Cells treated with 2DG and DNP (2DG/DNP) were heated at 42 degrees C for 20 min, and then incubated at 37 degrees C for up to 2h in the presence or absence of 2DG/DNP. In the absence of 2DG/DNP, the cellular ATP level recovered to 76% of the control level and DNA ladder formation was observed, whereas in the presence of 2DG/DNP, the cellular ATP level was further decreased (3-7% of the control) and no DNA fragmentation was detected. These results suggest that the inhibition of ATP synthesis is closely associated with the enhancement of sensitivity to heat and that ATP is required for the induction of apoptosis.

  11. Role of PTEN protein in spinal cord neurons in diabetic neuropathic pain in rats%脊髓神经元PTEN蛋白在大鼠糖尿病神经痛中的作用

    Institute of Scientific and Technical Information of China (English)

    黄振兴; 黄腾; 何万友; 贺俭; 王汉兵; 杨承祥; 周俊

    2016-01-01

    目的 探讨脊髓神经元第10号染色体同源缺失性磷酸酶张力蛋白基因(PTEN)蛋白在大鼠糖尿病神经痛中的作用.方法 雄性SD大鼠,2月龄,体重180 ~ 200 g,采用腹腔注射1%链脲佐菌素(STZ) 60 mg/kg的方法制备糖尿病模型.取糖尿病神经痛大鼠16只,采用随机数字表法,将其分为2组(n=8):糖尿病神经痛组(DNP组)和糖尿病神经痛+PTEN蛋白抑制剂bpv组(DPN-bpv组);另取16只大鼠,采用随机数字表法,将其分为2组(n=8):对照组(C组)和bpv组.DNP-bpv和bpv组于注射STZ后14-28 d时腹腔注射bpv 0.2 mg/kg,1次/d.分别于注射STZ前(T1)、注射STZ后2、7、14、21、28 d(T2-6)时测定机械缩足反应阈(MWT),测定MWT后处死大鼠,取L45脊髓组织,采用ELISA法检测PTEN蛋白活性,采用Western blot法检测丝氨酸/苏氨酸蛋白激酶(Akt)(s473)磷酸化水平.结果 与C组比较,DNP组和DNP-bpv组T4-6时MWT降低,脊髓PTEN蛋白活性和Akt(s473)磷酸化水平升高(P<0.05或0.01);与DNP组比较,DNP-bpv组T6时MWT升高,脊髓PTEN蛋白活性和Akt(s473)磷酸化水平降低(P<0.05).结论 脊髓神经元PTEN蛋白参与了大鼠糖尿病神经痛的维持.%Objective To investigate the role of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) protein in the spinal cord neurons in diabetic neuropathic pain (DNP) in rats.Methods Male Sprague-Dawley rats,aged 2 months,weighing 180-200 g,were studied.Diabetes mellitus was induced by single intraperitoneal injection of streptozotocin (STZ) 60 mg/kg.Sixteen rats with DNP were randomly divided into 2 groups (n =8 each) using a random number table:DNP group and DNP+PTEN inhibitor bpv (pic) group (DPN-bpv group).Another 16 rats were equally and randomly divided into either control group (group C) or bpv group.In DNP-bpv and bpv groups,bpv (pic) 0.2mg/kg was injected intraperitoneally once a day within 14-28 days after injection of STZ.Before STZ injection (T1),and at 2,7,14,21 and 28 days after STZ

  12. 加巴喷丁对糖尿病神经病理痛大鼠背根神经节Toll样受体4表达的影响%The effect of systemic administration of gabapentin on the expression of toll-like receptor 4 in dorsal root ganglion neurons in diabetic neuropathic pain rats

    Institute of Scientific and Technical Information of China (English)

    苗蓓; 殷悦; 周田田; 邵翠杰; 曹君利

    2013-01-01

    目的 探讨加巴喷丁(gabapentin,GBP)对糖尿病神经病理性疼痛(diabetic neuropathic pain,DNP)大鼠背根神经节(dorsal root ganglia,DRG)Toll样受体4(toll-like receptor 4,TLR4)表达的影响. 方法 108只成年健康雄性SD大鼠,采用完全随机分组法分为4组:对照组(C组)、模型组(DNP组)、生理盐水(SC)+DNP组和GBP+DNP组(每组27只).观察链脲佐菌素(streptozocin,STZ)注射前1d及注射后3、7、10、14、21、28 d测定缩足反射机械刺激阈值(paw withdrawal mechanical threshold,PWMT)和缩足反射热辐射潜伏期(paw withdrawal thermal latency,PWTL),SC+DNP组和GBP+DNP组于STZ注射15d起分别腹腔注射生理盐水或GBP 50 mg/kg,1次/d,连续7d,并于21、28 d测定PWMT和PWTL.行为学测试完成后选择21 d大鼠用免疫组化和Western blot方法检测大鼠DRG中TLR4的表达. 结果 与C组比较,DNP组PWMT (3.9±0.9)g降低,PWTL[(6.1±0.8)s]缩短,DRG中TLR4蛋白的表达显著上调(P<0.05);与DNP组比较,SC+DNP组大鼠在腹腔注射生理盐水后,PWMT、PWTL和TLR4蛋白的表达无明显改变(P>0.05);与DNP组和SC+DNP组比较,GBP+DNP组PWMT(8.3±0.8)g升高,PWTL[(9.9±1.2)s]延长,DRG中TLR4蛋白的表达下调(P<0.05). 结论 GBP可通过抑制DRG中TLR4表达,从而减轻大鼠DNP.%Objective To investigate the effect of systemic administration of gabapentin(GBP) on the expression of toll-like receptor 4(TLR4) in dorsal root ganglion neurons in a rat model of diabetic neuropathic pain (DNP).Methods One hundred and eight male SD rats weighing 200 g-220 g were randomly divided into 4 groups (n=27):control group (group C),DNP group,saline group (SC+DNP group)and gabapentin group(GBP+DNP group).Diabetes was induced with streptozocin(70 mg/kg) subcutaneously.Paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured at 1 d before and 3,7,10,14,21,28 d after streptozocin injection and 21,28 d after administration of GBP (50 mg

  13. Effect of curcumin on p-ERK1/2-AP-1 cascade and diabetic neuropathic pain in rats%p-ERK1/2-AP-1通路在姜黄素抗大鼠糖尿病神经病理性痛中的作用

    Institute of Scientific and Technical Information of China (English)

    黄葱葱; 陈果; 吴艳; 连庆泉; 李军; 曹红

    2011-01-01

    AIM: To evaluate the role of p - ERK1/2 - AP - 1 cascade in the process of curcumin against diabetic neuropathic pain ( DNP ) in rats.METHODS: Ninety - six male Sprague - Dawley rats were randomly divided into 4 groups ( n = 24 ): normal control group, DNP group, DNP with solvent group and DNP with curcumin ( 100 mg/kg ) group.The rat model of diabetes was induced by a single intraperitoneal injection of streptozotocin ( STZ, 75 mg/kg ).Mechanical allodynia and thermal hyperalgesia were tested by mechanical withdrawal threshold ( MWT ) and thermal withdrawal latency ( TWL ) 2 weeks after induction, respectively.The diabetic rats were treated with curcumin ( 100 mg·kg-1·d-1 , ip ) for 2 weeks.The conditions of hyperalgesia and allodynia were determined 2 d before STZ injection, 14 d after STZ injection, and 3 d, 7 d, 14 d after administered with curcumin.The change of p - ERK1/2 was measured by the methods of Western blotting and immunohistochemistry.The expression of AP - 1 in spinal cord dorsal horn and dorsal root ganglion ( DRG ) was detected by electromobility shift assay ( EMSA ).RESULTS: Compared with normal control group, the rats in DNP group developed hyperglycemia and a decrease in MWT and TWL associated with an increase in the activity of p - ERK1/ 2 and AP - 1 in dorsal horn and DRG( P <0.05 ).Compared with DNP group, 7 - day treatment with curcumin significantly attenuated mechanical allodynia and thermal hyperalgesia, and these effects were correlated with inhibiting the hyper -activation of p - ERK1/2 and AP - 1 14 days after treatment with curcumin ( P <0.05 ).CONCLUSION: Curcumin has beneficial effects on hyperalgesia in STZ - induced peripheral neuropathic pain.Activation of p - ERK1/2 and AP - 1 may be the key mechanism of DNP in spinal cord and DRG.%目的:观察p-ERK1/2-AP-1通路在姜黄素(Cur)抗大鼠糖尿病神经病理性痛(DNP)中的作用.方法:雄性SD大鼠96只,随机分为4组(n=24):正常对照组、DNP组、DNP+溶剂组(DNP+Sol组)和DNP

  14. Asymmetric optical nuclear spin pumping in a single uncharged quantum dot

    Energy Technology Data Exchange (ETDEWEB)

    Klotz, Florian; Jovanov, Vase; Kierig, Johannes; Clark, Emily; Bichler, Max; Abstreiter, Gerhard; Brandt, Martin; Finley, Jonathan [Walter Schottky Institut, Technische Universitaet Muenchen, Garching (Germany); Schwager, Heike; Giedke, Geza [Max-Planck-Institut fuer Quantenoptik, Garching (Germany)

    2011-07-01

    We present the observation of a unipolar optically pumped dynamic nuclear polarization (DNP) in a single self assembled InGaAs quantum dot (QD). Electrons are resonantly excited in the QD and polarize the nuclear spin system via the hyperfine contact coupling, creating an Overhauser magnetic field. Remarkably, we observe a strong asymmetry in nuclear spin pumping for excitation of the two Zeeman-split neutral exciton states. Hereby, pumping the higher energy Zeeman branch effectively polarizes the nuclear spin system, whereas the lower energy branch does not. We also find a characteristic dependence of the observed DNP on the applied magnetic field where optically induced nuclear spin pumping is most efficient for an intermediate regime of 4-6 T, with a polarization of the nuclear spin bath of 53%. A theoretical model is developed that successfully explains the empirically found features based on the exciton level structure of the system.

  15. Mutagenicity and Induction of Drug—metabolizing Enzyme Activity by LPG Combustion Particulates in Rats

    Institute of Scientific and Technical Information of China (English)

    YINXue-Jun; LIUJun-Zhuo; 等

    1994-01-01

    Methylene chloride extracts of particulates from liquefied petroleum gas(LPG)combustion appliance were studied by using Ames test,micronucleus test and inducibility of pulmonary and hepatic aryl hydrocarbon hydroxylase(AHH)and glutathione S-transferase(GST)in rats.The extracts showed mutagenicity for Salmonella typhimurium strain TA98 and its derivatives TA98NR and TA98/1,8-DNP6 with or without S9 mix.The revertants in strains TA98NR and TA98/1,8-DNP6 were less than 40%and 50% of than in strain TA98 without S9 mix,respectively.Positive results were obtained in mouse bone marrow micronucleus assay.Intratracheal instillation of the extracts led to increase in pulmonary(but not hepatic)AHH and GST activities in rats.In was seen that AHH was more sensitive than GST to induction by the extracts.

  16. ["E" rosette formation in "active" T lymphocytes: phenomenon modulated by intracellular level of cyclic AMP and GMP (author's transl)].

    Science.gov (United States)

    Machado, J A; Antunes, L J; Silva, E N

    1977-08-01

    The rosette formation envolving the binding of sheep red blood cells (SRBC) with active T lymphocytes was activated when the lymphocytes were incubated with levamisole, acetylcholine or carbamylcholine. Similar activation was seen when to the incubation medium was added substances of glucose metabolism (lactate, fumarate or succinate) or triphosphate de adenosina--ATP. The lymphocytes incubation with aminophyline, isoproterenol or 2-4-dinitrofenol--DNP--inhibited the rosette formation. The inhibition promoted by aminophyline was reversed by levamisole, acetylcholine or carbamylcholine, but not when lactate or ATP was used. When the rosette formation inhibition was caused by DNP, the reversion was only possible by ATP and no affect occurred if guanil cyclase activators were added to the incubation medium.

  17. Biomarkers of oral exposure to 3-nitro-1,2,4-triazol-5-one (NTO) and 2,4-dinitroanisole (DNAN) in blood and urine of rhesus macaques (Macaca mulatta).

    Science.gov (United States)

    Hoyt, Nathan; Brunell, Marla; Kroeck, Karl; Hable, Mike; Crouse, Lee; O'Neill, Art; Bannon, Desmond I

    2013-11-01

    The U.S. Department of Defense is using the chemicals 2,4-dinitroanisole (DNAN) and 3-nitro-1, 2,4-triazol-5-one (NTO) in new munitions development. In a screen for biomarkers of exposure, these compounds were measured in urine and blood of male rhesus monkeys after oral doses. NTO peaked at 4 h, with urinary concentrations at least 100-fold higher than that of blood or serum while 4-dinitrophenol (DNP), a metabolite of DNAN, appeared in blood at concentrations 10- to 20-fold higher than the parent compound. For human exposure monitoring, urine is optimal for NTO while the metabolite DNP in blood is best for DNAN.

  18. ParaHydrogen Induced Polarization of 13C carboxylate resonance in acetate and pyruvate.

    Science.gov (United States)

    Reineri, Francesca; Boi, Tommaso; Aime, Silvio

    2015-01-05

    The advent of nuclear spins hyperpolarization techniques represents a breakthrough in the field of medical diagnoses by magnetic resonance imaging. Dynamic nuclear polarization (DNP) is the most widely used method, and hyperpolarized metabolites such as [1-(13)C]-pyruvate are shown to report on status of tumours. Parahydrogen-induced polarization (PHIP) is a chemistry-based technique, easier to handle and much less expensive in respect to DNP, with significantly shorter polarization times. Its main limitation is the availability of unsaturated precursors for the target substrates; for instance, acetate and pyruvate cannot be obtained by direct incorporation of the parahydrogen molecule. Herein we report a method that allows us to achieve hyperpolarization in this kind of molecule by means of a tailored precursor containing a hydrogenable functionality that, after polarization transfer to the target (13)C moiety, is cleaved to obtain the metabolite of interest. The reported procedure can be extended to a number of other biologically relevant substrates.

  19. Photovoltaic Characteristics of a Naphthylamine Derivative

    Institute of Scientific and Technical Information of China (English)

    FU,Hui-Ying; SONG,Qun-Liang; SUN,Xiao-Yu; HOU,Xiao-Yuan; XIAO,Fei; SHAO,Bing-Xian

    2007-01-01

    Organic photovoltaic (OPV) cells were fabricated via vacuum vapor deposition with {4-[2-(3-dicyanomethylidene-5,5-dimethylcyclohexenyl)vinyl]phenyl}di(1-naphthyl)amine (DNP-2CN) as the electron donor,and fullerene (C60) as the electron acceptor. A thin film (10 nm) of tris(8-quinolinolato)aluminum (Alq3) was adopted as the buffer layer. A device based on this DNP-2CN exhibited an open circuit voltage (Voc) of 370 mV, a short-circuit current density (Jsc) of 0.61 mA·cm-2, and a white-light power conversion efficiency (η) of 0.09%(AM1.5, 75 mW·cm-2).

  20. Acute energy restriction triggers Wallerian degeneration in mouse

    DEFF Research Database (Denmark)

    Alvarez, Susana; Moldovan, Mihai; Krarup, Christian

    2008-01-01

    the neurotoxic effect of NO with the effect of the mitochondrial uncoupler 2,4-dinitrophenol (DNP) on electrically active axons of mouse sciatic nerve. The right tibial nerve was stimulated at the ankle. Muscle responses were recorded from plantar muscles and ascending nerve action potentials were recorded form...... the exposed sciatic nerve by means of a hook electrode. The sciatic nerve was focally immersed over a length of 1 cm in either phosphate buffered saline (PBS), a solution of approximately 4 microM NO obtained from 10 mM of the NO-donor DETA NONOate, or a solution of up to 1 mM DNP. Following 3 hours of 200 Hz...

  1. Post-Electrophoretic Identification of Oxidized Proteins

    Directory of Open Access Journals (Sweden)

    Conrad Craig

    2000-01-01

    Full Text Available The oxidative modification of proteins has been shown to play a major role in a number of human diseases. However, the ability to identify specific proteins that are most susceptible to oxidative modifications is difficult. Separation of proteins using polyacrylamide gel electrophoresis (PAGE offers the analytical potential for the recovery, amino acid sequencing, and identification of thousands of individual proteins from cells and tissues. We have developed a method to allow underivatized proteins to be electroblotted onto PVDF membranes before derivatization and staining. Since both the protein and oxidation proteins are quantifiable, the specific oxidation index of each protein can be determined. The optimal sequence and conditions for the staining process are (a electrophoresis, (b electroblotting onto PVDF membranes, (c derivatization of carbonyls with 2,4-DNP, (d immunostaining with anti DNP antibody, and (e protein staining with colloidal gold.

  2. Studies on the interactions of 2, 4-dinitrophenol and 2, 4-dichlorphenol with trypsin.

    Science.gov (United States)

    Zhang, Hong-Mei; Zhou, Qiu-Hua; Wang, Yan-Qing

    2010-03-01

    The interactions of 2, 4-dinitrophenol and 2, 4-dichlorphenol with trypsin were investigated by fluorescence, synchronous fluorescence, and three-dimensional fluorescence spectra techniques under physiological pH 7.40. The 2, 4-dinitrophenol and 2, 4-dichlorphenol effectively quenched the intrinsic fluorescence of trypsin via static quenching. The process of binding 2, 4-dinitrophenol and 2, 4-dichlorphenol with trypsin was a spontaneous molecular interaction procedure. The electrostatic repulsion does favor the interaction between 2, 4-DNP and trypsin. However, the interaction of 2, 4-DCP and trypsin can be explained on the basis of hydrogen bonding and van der Waals. The results of synchronous fluorescence spectroscopy and three-dimensional fluorescence spectra indicated that the structure of these trytophan and tyrosine residues environments were altered by 2, 4-DNP and 2, 4-DCP.

  3. Preparing emotionally intelligent doctor of nursing practice leaders.

    Science.gov (United States)

    Renaud, Michelle T; Rutledge, Carolyn; Shepherd, Laurel

    2012-08-01

    The Institute of Medicine (IOM) identified the need for interdisciplinary teams that collaborate, communicate, and integrate care across settings to improve health care delivery. Focusing on innovative strategies that address leadership skills in graduate nursing education could have an effect on interdisciplinary partnerships, transformation of patient care, and new styles of leadership to change current practice models. In response to the IOM guidelines, we incorporated emotional intelligence as a component in our Doctor of Nursing Practice (DNP) leadership curriculum. This article describes a new action-oriented leadership model that prepares the DNP graduate for leadership roles to serve the public and the nursing discipline during a time of radical changes in health care. Behavioral profile, nontraditional readings, and online discussions form the basis of the model. The principles and strategies in this article can be applied to nursing education in multiple arenas, at both the undergraduate and graduate settings.

  4. Magnetic resonance imaging without field cycling at less than earth's magnetic field

    Science.gov (United States)

    Lee, Seong-Joo; Shim, Jeong Hyun; Kim, Kiwoong; Yu, Kwon Kyu; Hwang, Seong-min

    2015-03-01

    A strong pre-polarization field, usually tenths of a milli-tesla in magnitude, is used to increase the signal-to-noise ratio in ordinary superconducting quantum interference device-based nuclear magnetic resonance/magnetic resonance imaging experiments. Here, we introduce an experimental approach using two techniques to remove the need for the pre-polarization field. A dynamic nuclear polarization (DNP) technique enables us to measure an enhanced resonance signal. In combination with a π / 2 pulse to avoid the Bloch-Siegert effect in a micro-tesla field, we obtained an enhanced magnetic resonance image by using DNP technique with a 34.5 μT static external magnetic field without field cycling. In this approach, the problems of eddy current and flux trapping in the superconducting pickup coil, both due to the strong pre-polarization field, become negligible.

  5. The Distributed Network Processor: a novel off-chip and on-chip interconnection network architecture

    CERN Document Server

    Biagioni, Andrea; Lonardo, Alessandro; Paolucci, Pier Stanislao; Perra, Mersia; Rossetti, Davide; Sidore, Carlo; Simula, Francesco; Tosoratto, Laura; Vicini, Piero

    2012-01-01

    One of the most demanding challenges for the designers of parallel computing architectures is to deliver an efficient network infrastructure providing low latency, high bandwidth communications while preserving scalability. Besides off-chip communications between processors, recent multi-tile (i.e. multi-core) architectures face the challenge for an efficient on-chip interconnection network between processor's tiles. In this paper, we present a configurable and scalable architecture, based on our Distributed Network Processor (DNP) IP Library, targeting systems ranging from single MPSoCs to massive HPC platforms. The DNP provides inter-tile services for both on-chip and off-chip communications with a uniform RDMA style API, over a multi-dimensional direct network with a (possibly) hybrid topology.

  6. Inhomogeneous dynamic nuclear polarization and suppression of electron polarization decay in a quantum dot

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Na [Department of Optical Science and Engineering, Fudan University, Shanghai 200433 (China); School of Physics and Technology, Wuhan University, Wuhan, Hubei 430072 (China); Ding, Wenkui; Shi, Anqi [School of Physics and Technology, Wuhan University, Wuhan, Hubei 430072 (China); Zhang, Wenxian, E-mail: wxzhang@whu.edu.cn [School of Physics and Technology, Wuhan University, Wuhan, Hubei 430072 (China)

    2016-08-12

    We investigate the dynamic nuclear polarization in a quantum dot. Due to the suppression of direct dipolar and indirect electron-mediated nuclear spin interactions by frequently injected electron spins, our analytical results under independent spin approximation agree well with quantum numerical simulations for a small number of nuclear spins. We find that the acquired nuclear polarization is highly inhomogeneous, proportional to the square of the local electron-nuclear hyperfine interaction constant. Starting from the inhomogeneously polarized nuclear spins, we further show that the electron polarization decay time can be extended 100 times even at a relatively low nuclear polarization. - Highlights: • Nuclear spin polarization is highly inhomogeneous after a dynamic nuclear polarization (DNP) process. • The DNP process is well described by an analytical model based on the independent spin approximation. • The highly inhomogeneous nuclear polarization significantly prolongs the electron spin coherence time.

  7. Batrachochytrium dendrobatidis infection of amphibians in the Doñana National Park, Spain.

    Science.gov (United States)

    Hidalgo-Vila, Judit; Díaz-Paniagua, Carmen; Marchand, Marc A; Cunningham, Andrew A

    2012-03-20

    Amphibian chytridiomycosis, caused by infection with the non-hyphal, zoosporic chytrid fungus Batrachochytrium dendrobatidis (Bd), is an emerging infectious disease recognised as a cause of recent amphibian population declines and extinctions worldwide. The Doñana National Park (DNP) is located in southwestern Spain, a country with widespread Bd infection. This protected area has a great diversity of aquatic habitats that constitute important breeding habitats for 11 native amphibian species. We sampled 625 amphibians in December 2007 and February to March 2008, months that correspond to the early and intermediate breeding seasons for amphibians, respectively. We found 7 of 9 sampled species to be infected with Bd and found differences in prevalence between sampling periods. Although some amphibians tested had higher intensities of infection than others, all animals sampled were apparently healthy and, so far, there has been no evidence of either unusually high rates of mortality or amphibian population declines in the DNP.

  8. THz-waves channeling in a monolithic saddle-coil for Dynamic Nuclear Polarization enhanced NMR.

    Science.gov (United States)

    Macor, A; de Rijk, E; Annino, G; Alberti, S; Ansermet, J-Ph

    2011-10-01

    A saddle coil manufactured by electric discharge machining (EDM) from a solid piece of copper has recently been realized at EPFL for Dynamic Nuclear Polarization enhanced Nuclear Magnetic Resonance experiments (DNP-NMR) at 9.4 T. The corresponding electromagnetic behavior of radio-frequency (400 MHz) and THz (263 GHz) waves were studied by numerical simulation in various measurement configurations. Moreover, we present an experimental method by which the results of the THz-wave numerical modeling are validated. On the basis of the good agreement between numerical and experimental results, we conducted by numerical simulation a systematic analysis on the influence of the coil geometry and of the sample properties on the THz-wave field, which is crucial in view of the optimization of DNP-NMR in solids.

  9. Consenso experto sobre el uso clínico de los tratamientos por vía tópica en el manejo del dolor neuropático periférico

    Directory of Open Access Journals (Sweden)

    C. Pérez

    2013-12-01

    Full Text Available Objetivo: Proponer a partir del consenso de un panel de expertos de ámbito estatal que integre la experiencia clínica y la evidencia disponible más actual, recomendaciones sobre el uso clínico de los tratamientos por vía tópica para el manejo del dolor neuropático periférico (DNP. Métodos: Se proponen, a partir de una revisión bibliográfica sobre las distintas opciones terapéuticas tópicas en DNP, una serie de criterios profesionales y recomendaciones clínicas para la mejora del uso de dichos agentes tópicos. Se empleó el método Delphi modificado en dos rondas para contrastar las opiniones de un panel nacional de 52 reconocidos expertos, seleccionados mediante una estrategia en "bola de nieve" de entre el colectivo de anestesiólogos de unidades del dolor (94 % y otros especialistas (neurólogos y traumatólogos. Se evaluaron 61 recomendaciones clínicas agrupadas en 6 áreas temáticas: a DNP: tratamiento tópico versus sistémico (11 ítems; b dolor neuropático postquirúrgico, postraumático y muñones dolorosos (12 ítems; c neuralgia posherpética, intercostal y del trigémino (9 ítems; d DNP por atrapamiento (8 ítems; e síndrome de dolor regional complejo (11 ítems; y f neuropatía diabética (ND y otras polineuropatías (por VIH, alcohol, toxicidad, etc. (10 ítems. Se empleó una escala ordinal de tipo Likert de 9 puntos (desacuerdo/acuerdo para evaluar cada recomendación. Tras la primera ronda de encuesta, se facilitó al panel información del resultado (resultados estadísticos y opiniones libres de los panelistas y se solicitó la reconsideración del voto sobre los ítems no consensuados. Resultados: Tras la primera ronda del panel se logró consenso en 37 de las 61 cuestiones planteadas. Al final de la segunda ronda el acuerdo ascendió hasta 46 ítems (75 %. En general, se aprecia consenso entre los expertos sobre la conveniencia de introducir los tratamientos tópicos en primera línea de tratamiento del

  10. Suppression of unprimed T and B cells in antibody responses by irradiation-resistant and plastic-adherent suppressor cells in Toxoplasma gondii-infected mice

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Y.; Kobayashi, A.

    1983-04-01

    In the acute phase of Toxoplasma infection, the function of both helper T and B cells was suppressed in primary antibody responses to dinitrophenol (DNP)-conjugated protein antigens. During the course of infection, the suppressive effect on T cells seems to continue longer than that on B cells, since suppression in responses to sheep erythrocytes, a T-dependent antigen, persisted longer than those to DNP-Ficoll, a T-independent antigen. Plastic-adherent cells from the spleens of Toxoplasma-infected and X-irradiated (400 rads) mice had strong suppressor activity in primary anti-sheep erythrocyte antibody responses of normal mouse spleen cells in vitro. These data suggest that the activation of irradiation-resistant and plastic-adherent suppressor cells causes the suppression of both T and B cells in Toxoplasma-infected mice.

  11. Evidence for Rapid Oxidative Phosphorylation and Lactate Fermentation in Motile Human Sperm by Hyperpolarized (13)C Magnetic Resonance Spectroscopy.

    Science.gov (United States)

    Reynolds, Steven; Ismail, Nurul Fadhlina Bt; Calvert, Sarah J; Pacey, Allan A; Paley, Martyn N J

    2017-06-28

    Poor sperm motility is a common cause of male infertility for which there are no empirical therapies. Sperm motility is powered by adenosine triphosphate but the relative importance of lactate fermentation and Oxidative Phosphorylation (OxPhos) is debated. To study the relationship between energy metabolism and sperm motility we used dissolution Dynamic Nuclear Polarization (dDNP) for the first time to show the rapid conversion of (13)C1-pyruvate to lactate and bicarbonate, indicating active glycolytic and OxPhos metabolism in sperm. The magnitude of both lactate and bicarbonate signals were positively correlated with the concentration of progressively motile sperm. After controlling for sperm concentration, increased progressive sperm motility generated more pyruvate conversion to lactate and bicarbonate. The technique of dDNP allows 'snapshots' of sperm metabolism to be tracked over the different stages of their life. This may provide help to uncover the causes of poor sperm motility and suggest new approaches for novel treatments or therapies.

  12. Dynamic nuclear polarization and optimal control spatial-selective 13C MRI and MRS

    DEFF Research Database (Denmark)

    Vinding, Mads Sloth; Laustsen, Christoffer; Maximov, Ivan I.

    2013-01-01

    Aimed at 13C metabolic magnetic resonance imaging (MRI) and spectroscopy (MRS) applications, we demonstrate that dynamic nuclear polarization (DNP) may be combined with optimal control 2D spatial selection to simultaneously obtain high sensitivity and well-defined spatial restriction. This is ach......Aimed at 13C metabolic magnetic resonance imaging (MRI) and spectroscopy (MRS) applications, we demonstrate that dynamic nuclear polarization (DNP) may be combined with optimal control 2D spatial selection to simultaneously obtain high sensitivity and well-defined spatial restriction....... This is achieved through the development of spatial-selective single-shot spiral-readout MRI and MRS experiments combined with dynamic nuclear polarization hyperpolarized [1-13C]pyruvate on a 4.7T pre-clinical MR scanner. The method stands out from related techniques by facilitating anatomic shaped region...

  13. Formulation and utilization of choline based samples for dissolution dynamic nuclear polarization

    DEFF Research Database (Denmark)

    Bowen, Sean; Ardenkjær-Larsen, Jan Henrik

    2013-01-01

    Hyperpolarization by the dissolution dynamic nuclear polarization (DNP) technique permits the generation of high spin polarization of solution state. However, sample formulation for dissolution-DNP is often difficult, as concentration and viscosity must be optimized to yield a dissolved sample...... with sufficient concentration, while maintaining polarization during the dissolution process. The unique chemical properties of choline permit the generation of highly soluble salts as well as deep eutectic mixtures with carboxylic acids and urea. We describe the formulation of these samples and compare...... their performance to more traditional sample formulations. Choline yields stable samples with exceptional polarization performance while simultaneously offering the capability to easily remove the choline after dissolution, perform experiments with the hyperpolarized choline, or anything in between....

  14. Innovación en hidrocarburos en Colombia

    Directory of Open Access Journals (Sweden)

    Juan Benavides Estévez-Bretón

    2011-01-01

    Full Text Available Este artículo presenta parte del Proyecto DNP- Uniandes sobre Clusters en la industria minero energética. El análisis se centrará en la innovación en hidrocarburos: primero, el planteamiento básico; segundo, los retos y oportunidades, y tercero, las líneas de acción en las que Colombia puede trabajar.//This article presents a fragment of the DNP- Uniandes project on Clusters in the energy mining industry. Specifically, the analysis focuses on innovation in hydrocarbons, starting with the project's basic approach. Next, it exposes the challenges and opportunities present in the Colombian context. Finally, it discusses the lines of action within which Colombia will move forward.

  15. Development of high voltage power supply for the KSTAR 170 GHz ECH and CD system

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, J.H., E-mail: jhjeong@nfri.re.kr [National Fusion Research Institute, Daejeon (Korea, Republic of); Bae, Y.S.; Joung, M.; Kim, H.J.; Park, S.I.; Han, W.S.; Kim, J.S.; Yang, H.L.; Kwak, J.G. [National Fusion Research Institute, Daejeon (Korea, Republic of); Sakamoto, K.; Kajiwara, K.; Oda, Y.; Hayashi, K. [Japan Atomic Energy Agency, Naka (Japan)

    2013-06-15

    Highlights: • A 3.6 MW (−66 kV/55 A) gyrotron power supply system was developed for the 170 GHz ECH system in KSTAR. • The main power supply includes a total of 32 PSM based HV power supply modules. • The voltage regulation of individual HV power module and LV power module is 3 kV and 0.5 kV, respectively. • The gyrotron is protected by means of a fast solid-state switch (MOS-FET). • The HV switching system can turn off the 60 kV to the cathode within 3 μs in the event of gyrotron faults. -- Abstract: A 3.6 MW (66 kV/55 A) DC power supply system was developed for the 170 GHz EC H and CD system in KSTAR. The power supply system consists of a cathode power supply (CPS), an anode power supply (APS) and a body power supply (BPS). The cathode power supply is capable of supplying a maximum voltage of −66 kV and a current of 55 A to the cathode with respect to the collector using pulse step modulation (PSM). The high voltage switching system for the cathode is made by a fast MOS-FET solid-state switch which can turn off the high voltage to the cathode within 3 μs in the occurrence of gyrotron faults. The APS is a voltage divider system consisting of a fixed resistor and zener diode units with the capability of 60 kV stand-off voltage. The anode voltage with respect to the cathode is controlled in a range of 0–60 kV by turning the MOS-FET switches connected in parallel to each zener diode on and off. For high frequency current modulation of the gyrotron, the parallel discharge switch is introduced between the cathode and anode in order to clamp the charged voltage in the stray capacitance. The BPS is a DC power supply with the capability of 50 kV/160 mA. The nominal operation parameter of BPS was 23 kV and 10 mA, respectively, and the voltage output is regulated with a stability of 0.025% of the rated voltage. The series MOS-FET solid-state switch is used for on/off modulation in the body voltage sychronizing with anode voltage. The parallel discharge

  16. Inhibition of oxidative phosphorylation for enhancing citric acid production by Aspergillus niger

    OpenAIRE

    Wang, Lu; Zhang, Jianhua; Cao, Zhanglei; Wang, Yajun; Gao, Qiang; Zhang, Jian; Wang, Depei

    2015-01-01

    Background The spore germination rate and growth characteristics were compared between the citric acid high-yield strain Aspergillus niger CGMCC 5751 and A. niger ATCC 1015 in media containing antimycin A or DNP. We inferred that differences in citric acid yield might be due to differences in energy metabolism between these strains. To explore the impact of energy metabolism on citric acid production, the changes in intracellular ATP, NADH and NADH/NAD+ were measured at various fermentation s...

  17. Thermal Analysis of Whole Bacterial Cells Exposed to Potassium Permanganate Using Differential Scanning Calorimetry: a Biphasic Dose-Dependent Response to Stress

    Directory of Open Access Journals (Sweden)

    Marina K. Abuladze

    2009-01-01

    Full Text Available Differential scanning calorimetry (DSC was applied to estimate the impact of the toxic oxidant potassium permanganate (PM on the intracellular structural and functional alterations at whole cell level using soil bacteria Arthrobacter oxydans as a model culture. Differential scanning calorimetry (DSC was applied in order to estimate the impact of the toxic oxidant potassium permanganate (PM on the intracellular structural and functional alterations at the whole cell level using the soil bacteria Arthrobacter oxydans as a model culture. We compared the total melting heat and the temperature of DNA-protein complex (DNP melting at the PM application prior to the calorimetry measurement and after 24-h exposure at the concentration range 0.02–1.4 mM. The initial oxidative effect caused changes in the pattern of the whole cell melting spectra (mainly at the temperature range 56–78°C, the decrease of Tmax °C DNP melting, and did not influence significantly the total heat of bacterial melting at different concentrations of PM. The prolonged effect of permanganate up to 24 h was characterized by a biphasic dose-dependent response to stress estimated by the DSC technique and the colony-forming assay. The low doses of PM (0.02 and 0.2 mM stimulated cell proliferation, and increased the total whole cell melting heat and the temperature of DNP melting. The toxic effect of PM up to 0.04 mM reduced cell viability, changed the character of multipeaked thermograms, and lowered the total melting heat and the temperature of DNP melting in a concentration-dependent manner. This study presents the DSC method for evaluating and monitoring the effects of exposure to potential human and environmental toxicants.

  18. Produção de álcoois superiores por linhagens de Saccharomyces durante a fermentação alcoólica Production of higher alcohols by Saccharomyces strains during alcoholic fermentation

    Directory of Open Access Journals (Sweden)

    L.E. Gutierrez

    1993-12-01

    Full Text Available A produção de álcoois superiores pelas leveduras Saccharomyces cerevisiae M-300-A, Saccharomyces uvarum IZ-1904 e levedura de panificação (Saccharomyces cerevisiae foi estudada em diversas condições de temperatura, concentração de sacarose, pH, fontes de nitrogênio e com inibidor 2-4 dinitrofenol (DNP. Em todas as condições estudadas, a levedura Saccharomyces uvarum IZ-1904 apresentou a menor formação de álcoois superiores enquanto a levedura de panifícação apresentou os teores mais elevados. Com o aumento de temperatura e da concentração de sacarose ocorreu maior formação de álcool isoamílico pelas leveduras estudadas. Em pH 4,5 ocorreu menor produção de álcoois superiores do que em pH 3,0. Na presença do inibidor DNP ocorreu significativa redução (pThe production of higher alcohols by Saccharomyces cerevisiae M-300-A, Saccharomyces uvarum IZ-1904 and baker's yeast (5. cerevisiae was studied under several temperature conditions, sucrose level, pH, nitrogen sources and with 2-4 dinitrophenol (DNP. The yeast IZ-1904 showed lower production of higher alcohols than other yeasts in all conditions studied. With the increase of temperature and higher level of sucrose an increase of isoamyl alcohol production was observed. A lower formation of higher alcohols was observed at pH 4.5 than at pH 3.0. With the addition of DNP occurred a significant reduction in isoamyl alcohol content. The yeasts did not show the sanie production of higher alcohols in relation to urea and ammonium sulfate.

  19. Impact of the Shodan Computer Search Engine on Internet-facing Industrial Control System Devices

    Science.gov (United States)

    2014-03-27

    processing unit CTO Chief Technology Officer DCS distributed control system DHS Department of Homeland Security DMZ demilitarized zones DNP3 distributed...updates” [22]. Eric Byres, Chief Technology Officer ( CTO ) of Belden’s Tofino Security, stated one vendor his firm works with estimates that less than 10...allowing remote control system monitoring and modification. Web management server reconnaissance is defined by a manual traversal and specific site

  20. Study of Adversarial and Defensive Components in an Experimental Machinery Control Systems Laboratory Environment

    Science.gov (United States)

    2014-09-01

    GNU Radio , iPDC, PMU Simulator, Saleae Logic, and Termineter). These tools enhance the vulnerability identification and security assessment of energy...Name INL Kali SamuraiSTFU Developer System(s) OpenDNP3 x Automatak Cross-platform Energy Sector Tools GNU Radio x Eric Blossom / GNU Project...code, Revision Number, as well as the Device IP. https://github.com/digitalbond/Redpoint GNU Radio Eric Blossom / GNU Project Cross

  1. Coal and char studies by advanced EMR techniques

    Energy Technology Data Exchange (ETDEWEB)

    Belford, R.L.; Clarkson, R.B.; Odintsov, B.M.

    1999-03-31

    Advanced magnetic resonance (EMR) methods are used to examine properties of coals, chars, and molecular species related to constituents of coal. During this grant period, further progress was made on proton NMR and low-frequency dynamic nuclear polarization (DNP) to examine the interaction between fluids such as water and the surface of suspended char particles. Effects of char particle size and type on water nuclear spin relaxation, T2, were measured and modeled.

  2. A Secure, Intelligent, and Smart-Sensing Approach for Industrial System Automation and Transmission over Unsecured Wireless Networks

    Directory of Open Access Journals (Sweden)

    Aamir Shahzad

    2016-03-01

    Full Text Available In Industrial systems, Supervisory control and data acquisition (SCADA system, the pseudo-transport layer of the distributed network protocol (DNP3 performs the functions of the transport layer and network layer of the open systems interconnection (OSI model. This study used a simulation design of water pumping system, in-which the network nodes are directly and wirelessly connected with sensors, and are monitored by the main controller, as part of the wireless SCADA system. This study also intends to focus on the security issues inherent in the pseudo-transport layer of the DNP3 protocol. During disassembly and reassembling processes, the pseudo-transport layer keeps track of the bytes sequence. However, no mechanism is available that can verify the message or maintain the integrity of the bytes in the bytes received/transmitted from/to the data link layer or in the send/respond from the main controller/sensors. To properly and sequentially keep track of the bytes, a mechanism is required that can perform verification while bytes are received/transmitted from/to the lower layer of the DNP3 protocol or the send/respond to/from field sensors. For security and byte verification purposes, a mechanism needs to be proposed for the pseudo-transport layer, by employing cryptography algorithm. A dynamic choice security buffer (SB is designed and employed during the security development. To achieve the desired goals of the proposed study, a pseudo-transport layer stack model is designed using the DNP3 protocol open library and the security is deployed and tested, without changing the original design.

  3. Influence of growth regulators and respiration inhibitors on dark transformation of phytochrome in coleoptiles of oat seedlings

    Directory of Open Access Journals (Sweden)

    Jan Kopcewicz

    2014-02-01

    Full Text Available Irradiation with red light leads to the formation of an unstable, undergoing gradual destruction, physiologically active PFR form of phytochrome in the coleoptiles of oat seedlings. Growth substances: IAA, GA3, kinetin, ABA, ethrel as well acetylcholine do not influence the nature and rate of phytochrome dark transformation. Inhibitors of energy-producing processes such as KCN, 2,4-DNP, DCCD and antimycin A inhibit the process of dark destruction of the PFR form of phytochrome.

  4. Increase in signal-to-noise ratio of > 10,000 times in liquid-state NMR

    Science.gov (United States)

    Ardenkjær-Larsen, Jan H.; Fridlund, Björn; Gram, Andreas; Hansson, Georg; Hansson, Lennart; Lerche, Mathilde H.; Servin, Rolf; Thaning, Mikkel; Golman, Klaes

    2003-09-01

    A method for obtaining strongly polarized nuclear spins in solution has been developed. The method uses low temperature, high magnetic field, and dynamic nuclear polarization (DNP) to strongly polarize nuclear spins in the solid state. The solid sample is subsequently dissolved rapidly in a suitable solvent to create a solution of molecules with hyperpolarized nuclear spins. The polarization is performed in a DNP polarizer, consisting of a super-conducting magnet (3.35 T) and a liquid-helium cooled sample space. The sample is irradiated with microwaves at 94 GHz. Subsequent to polarization, the sample is dissolved by an injection system inside the DNP magnet. The dissolution process effectively preserves the nuclear polarization. The resulting hyperpolarized liquid sample can be transferred to a high-resolution NMR spectrometer, where an enhanced NMR signal can be acquired, or it may be used as an agent for in vivo imaging or spectroscopy. In this article we describe the use of the method on aqueous solutions of [13C]urea. Polarizations of 37% for 13C and 7.8% for 15N, respectively, were obtained after the dissolution. These polarizations correspond to an enhancement of 44,400 for 13C and 23,500 for 15N, respectively, compared with thermal equilibrium at 9.4 T and room temperature. The method can be used generally for signal enhancement and reduction of measurement time in liquid-state NMR and opens up for a variety of in vitro and in vivo applications of DNP-enhanced NMR. HR ALIGN=LEFT WIDTH=50% NOSHADE SIZE=1>

  5. Temperature dependence of cross-effect dynamic nuclear polarization in rotating solids

    DEFF Research Database (Denmark)

    Geiger, Michel-Andreas; Orwick-Rydmark, Marcella; Märker, Katharina

    2016-01-01

    Dynamic nuclear polarization exploits electron spin polarization to boost signal-to-noise in magic-angle-spinning (MAS) NMR, creating new opportunities in materials science, structural biology, and metabolomics studies. Since protein NMR spectra recorded under DNP conditions can show improved...... the importance of protons in a sphere of 4-6 Å around the nitroxyl group, presumably for polarization pickup from electron spins....

  6. Prediction of Physicochemical Properties of Energetic Materials for Identification of Treatment Technologies for Waste Streams

    Science.gov (United States)

    2010-11-01

    advanced oxidation processes, chemical reduction processes, and bioremediation processes. Alkaline hydrolysis has been ex- amined as a treatment method...Physical Separation Processes Bioremediation Oxidation Reduction Adsorption, Extraction, Membrane DNAN AH (M) Ex (H); Ad(I) I DNP AH (M) Ex (M); Ad...2002. Prediction of aqueous solubility of drugs and pesticides with COSMO-RS. J. Computational Chemistry. 23(2):275-281. Klamt, A., F. Eckert, and M

  7. Degradation of 2,4-dinitrophenol using a combination of hydrodynamic cavitation, chemical and advanced oxidation processes.

    Science.gov (United States)

    Bagal, Manisha V; Gogate, Parag R

    2013-09-01

    In the present work, degradation of 2,4-dinitrophenol (DNP), a persistent organic contaminant with high toxicity and very low biodegradability has been investigated using combination of hydrodynamic cavitation (HC) and chemical/advanced oxidation. The cavitating conditions have been generated using orifice plate as a cavitating device. Initially, the optimization of basic operating parameters have been done by performing experiments over varying inlet pressure (over the range of 3-6 bar), temperature (30 °C, 35 °C and 40 °C) and solution pH (over the range of 3-11). Subsequently, combined treatment strategies have been investigated for process intensification of the degradation process. The effect of HC combined with chemical oxidation processes such as hydrogen peroxide (HC/H2O2), ferrous activated persulfate (HC/Na2S2O8/FeSO4) and HC coupled with advanced oxidation processes such as conventional Fenton (HC/FeSO4/H2O2), advanced Fenton (HC/Fe/H2O2) and Fenton-like process (HC/CuO/H2O2) on the extent of degradation of DNP have also been investigated at optimized conditions of pH 4, temperature of 35 °C and inlet pressure of 4 bar. Kinetic study revealed that degradation of DNP fitted first order kinetics for all the approaches under investigation. Complete degradation with maximum rate of DNP degradation has been observed for the combined HC/Fenton process. The energy consumption analysis for hydrodynamic cavitation based process has been done on the basis of cavitational yield. Degradation intermediates have also been identified and quantified in the current work. The synergistic index calculated for all the combined processes indicates HC/Fenton process is more feasible than the combination of HC with other Fenton like processes.

  8. Developing an Acquisition Strategy for the Colombian Navy’s New Strategic Surface Ships

    Science.gov (United States)

    2007-06-01

    37 Departamento Nacional de Planeacion (2006), “Visión Colombia II Centenario: 2019.” Retrieved 14 May 2007 from http...40 Jefatura Oficina Planeacion A.R.C. (July 2006), Plan de Desarrollo 2007–2010, conference presented at Dirección General Marítima, Bogota...Departamento Nacional de Planeacion (2006). “Visión Colombia II Centenario: 2019”. Retrieved 14 May 2007 from http://www.dnp.gov.co/paginas_detalle.aspx?idp=888

  9. A Secure, Intelligent, and Smart-Sensing Approach for Industrial System Automation and Transmission over Unsecured Wireless Networks

    Science.gov (United States)

    Shahzad, Aamir; Lee, Malrey; Xiong, Neal Naixue; Jeong, Gisung; Lee, Young-Keun; Choi, Jae-Young; Mahesar, Abdul Wheed; Ahmad, Iftikhar

    2016-01-01

    In Industrial systems, Supervisory control and data acquisition (SCADA) system, the pseudo-transport layer of the distributed network protocol (DNP3) performs the functions of the transport layer and network layer of the open systems interconnection (OSI) model. This study used a simulation design of water pumping system, in-which the network nodes are directly and wirelessly connected with sensors, and are monitored by the main controller, as part of the wireless SCADA system. This study also intends to focus on the security issues inherent in the pseudo-transport layer of the DNP3 protocol. During disassembly and reassembling processes, the pseudo-transport layer keeps track of the bytes sequence. However, no mechanism is available that can verify the message or maintain the integrity of the bytes in the bytes received/transmitted from/to the data link layer or in the send/respond from the main controller/sensors. To properly and sequentially keep track of the bytes, a mechanism is required that can perform verification while bytes are received/transmitted from/to the lower layer of the DNP3 protocol or the send/respond to/from field sensors. For security and byte verification purposes, a mechanism needs to be proposed for the pseudo-transport layer, by employing cryptography algorithm. A dynamic choice security buffer (SB) is designed and employed during the security development. To achieve the desired goals of the proposed study, a pseudo-transport layer stack model is designed using the DNP3 protocol open library and the security is deployed and tested, without changing the original design. PMID:26950129

  10. Development of Silicon-Coated Superparamagnetic Iron Oxide Nanoparticles for Targeted Molecular Imaging and Hyperthermic Therapy of Prostate Cancer

    Science.gov (United States)

    2015-08-01

    and directed to the sample via waveguide and slot antenna . Quality control was monitored using an on-board miniature NMR spectrometer to sample 29Si...were directed to the sample tube using a waveguide and slot antenna . The sample also resides within an in situ NMR coil, allowing quality assurance...using a miniature NMR spectrometer. In situ NMR studies to monitor the buildup of 29Si signal during DNP used a saturation recovery pulse sequence

  11. Selective Imaging of Malignant Ascites in a Mouse Model of Peritoneal Metastasis Using in Vivo Dynamic Nuclear Polarization-Magnetic Resonance Imaging.

    Science.gov (United States)

    Eto, Hinako; Hyodo, Fuminori; Nakano, Kenji; Utsumi, Hideo

    2016-02-16

    The presence of malignant ascites in advanced cancer patients is associated with both a poor prognosis and quality of life with a risk of abdominal infection and sepsis. Contemporary noninvasive visualization methods such as ultrasound, computed tomography, and magnetic resonance imaging (MRI) often struggle to differentiate malignant ascites from surrounding tissues. This study aimed to determine the utility of selective H2O imaging in the abdominal cavity with a free radical probe and deuterium oxide (D2O) contrast agent using in vivo dynamic nuclear polarization-MRI (DNP-MRI). Phantom imaging experiments established a linear relationship between H2O volume and image intensity using in vivo DNP-MRI. Similar results were obtained when the radical-D2O probe was used to determine selective and spatial information on H2O in vivo, modeled by the injection of saline into the abdominal cavity of mice. To demonstrate the utility of this method for disease, malignant ascites in peritoneal metastasis animal model was selected as one of the typical examples. In vivo DNP-MRI of peritoneal metastasis animal model was performed 7-21 days after intraperitoneal injection of luciferase, stably expressing the human pancreatic carcinoma (SUIT-2). The image intensity with increasing malignant ascites was significantly increased at days 7, 16, and 21. This increase corresponded to in vivo tumor progression, as measured by bioluminescent imaging. These results suggest that H2O signal enhancement in DNP-MRI using radical-D2O contrast is positively associated with the progression of dissemination and could be a useful biomarker for malignant ascites with cancer metastasis.

  12. Simultaneous biodegradation of nitrogen-containing aromatic compounds in a sequencing batch bioreactor

    Institute of Scientific and Technical Information of China (English)

    LIU Xing-yu; WANG Bao-jun; JIANG Cheng-ying; ZHAO Ke-xin; Harold L.Drake; LIU Shuang-Jiang

    2007-01-01

    Many nitrogen-containing aromatic compounds (NACs), such as nitrobenzene (NB), 4-nitrophenol (4-NP), aniline (AN), and 2,4-dinitrophenol (2,4-DNP), are environmentally hazardous, and their removal from contaminated water is one of the main challenges facing wastewater treatment plants. In this study, synthetic wastewater containing NB, 4-NP, 2,4-DNP, and AN at concentrations ranging from 50 to 180 mg/L was fed into a sequencing batch reactor (SBR). Analyses of the SBR system indicated that it simultaneously removed more than 99% of the NACs at loading rates of 0.36 kg NB/(m3·d), 0.3 kg 4-NP/(m3·d), 0.25 kg AN/(m3·d), and 0.1 kg 2,4-DNP/(m3·d). Bacterial groups of Bacteriodetes, Candidate division TM7, α-Proteobacteria, and β-Proteobacteria were dominant in the clone libraries of 16S rRNA genes retrieved from the microbial communities in the SBR system. "Cycle tests" designed to alter feeding and aeration parameters of the SBR system demonstrated that the resident microbial biome of the SBR system responded rapidly to changing conditions. Consumption of O2 was concomitant with the apparent mineralization of NACs. Aromatic ring-cleaving dioxygenase activities suggested that (1) AN and NB were degraded via catechol 2,3-dioxygenase; (2) 4-NP was degraded via 1,2,4-benzentriol 1,2-dioxygenase; and (3) 2,4-DNP was degraded via an unresolved pathway.

  13. UCP2- and non-UCP2-mediated electric current in eukaryotic cells exhibits different properties.

    Science.gov (United States)

    Wang, Ruihua; MoYung, K C; Zhang, M H; Poon, Karen

    2015-12-01

    Using live eukaryotic cells, including cancer cells, MCF-7 and HCT-116, normal hepatocytes and red blood cells in anode and potassium ferricyanide in cathode of MFC could generate bio-based electric current. Electrons and protons generated from the metabolic reaction in both cytosol and mitochondria contributing to the leaking would mediate the generation of electric current. Both resveratrol (RVT) and 2,4-dinitrophenol (DNP) used to induce proton leak in mitochondria were found to promote electric current production in all cells except red blood cells without mitochondria. Proton leak might be important for electric current production by bringing the charge balance in cells to enhance the further electron leak. The induced electric current by RVT can be blocked by Genipin, an inhibitor of UCP2-mediated proton leak, while that induced by DNP cannot. RVT could reduce reactive oxygen species (ROS) level in cells better than that of DNP. In addition, RVT increased mitochondrial membrane potential (MMP), while DNP decreased it. Results highly suggested the existence of at least two types of electric current that showed different properties. They included UCP2-mediated and non-UCP2-mediated electric current. UCP2-mediated electric current exhibited higher reactive oxygen species (ROS) reduction effect per unit electric current production than that of non-UCP2-mediated electric current. Higher UCP2-mediated electric current observed in cancer cells might contribute to the mechanism of drug resistence. Correlation could not be established between electric current production with either ROS and MMP without distinguishing the types of electric current.

  14. Dynamic Nuclear Polarization and Relaxation of H and D Atoms in Solid Mixtures of Hydrogen Isotopes

    Science.gov (United States)

    Sheludiakov, S.; Ahokas, J.; Järvinen, J.; Vainio, O.; Lehtonen, L.; Vasiliev, S.; Lee, D. M.; Khmelenko, V. V.

    2016-12-01

    We report on a study of dynamic nuclear polarization and electron and nuclear spin relaxation of atomic hydrogen and deuterium in solid molecular matrices of H2, D2 , and HD mixtures. The electron and nuclear spin relaxation times (T_{1e} and T_{1N} ) were measured within the temperature range 0.15-2.5 K in a magnetic field of 4.6 T, conditions which ensure a high polarization of electron spins. We found that T_{1e} is nearly temperature independent in this temperature range, while T_{1N} decreased by two orders of magnitude upon raising temperature. Such strong temperature dependence is typical for the nuclear Orbach mechanism of relaxation via the electron spins. We found that the nuclear spins of H atoms in solid D2 and D2{:}HD can be efficiently polarized by the Overhauser effect. Pumping the forbidden transitions of H atoms also leads to DNP, with the efficiency strongly dependent on the concentration of D atoms. This behavior indicates the cross effect mechanism of the DNP and nuclear relaxation, which turns out to be well resolved in the conditions of our experiments. Efficient DNP of H atoms was also observed when pumping the middle D line located in the center of the ESR spectrum. This phenomenon can be explained in terms of clusters or pairs of H atoms with a strong exchange interaction. These clusters have partially allowed transitions in the center of the ESR spectrum, and DNP may be created via the resolved cross effect.

  15. Using Standardized Patients to Teach Interprofessional Competencies to Dental Students.

    Science.gov (United States)

    Anders, Patrick L; Scherer, Yvonne Krall; Hatton, Michael; Antonson, Donald; Austin-Ketch, Tammy; Campbell-Heider, Nancy

    2016-01-01

    The aims of this study were to develop, implement, and evaluate a novel interprofessional standardized patient exercise (ISPE) with oral-systemic and interprofessional collaborative practice (IPCP) components. Dental students and doctor of nursing practice (DNP) students at one U.S. university participated in the simulation, which was primarily designed to test their teamwork skills. In spring 2014, DNP students worked in the dental clinics with dental students under the supervision of nursing and dental faculty members. To test the teamwork outcomes for both groups of students, a standardized patient (SP) scenario was designed to include multiple chronic medical diagnoses and an oral-systemic component. The exercise was filmed for later review. Outcomes measures included SP and student self-evaluations and faculty evaluation of student documentation. The primary outcome of interest from a dental standpoint was faculty evaluation of IPCP competencies derived from the Core Competencies of Interprofessional Collaborative Practice and were deemed to be observable by faculty when viewing the videotaped scenario. Eight teams of students participated with an SP trained in the scenario. Each team consisted of a DNP student, a fourth-year dental student, and a second-year dental student. All eligible students in the DNP class (n=20) and eight students from each dental class (approximately 110 each) participated. The results showed that the teams scored highest on the role/responsibilities subscale, indicating students were respectful of each other's roles and expertise and effectively engaged each other to develop strategies to meet the patient's needs. Scores on the three other subscales (values/ethics, interprofessional communication, and teams/teamwork) were also high. These findings appeared to support IPCP as a method to foster knowledge and respect for other roles and responsibilities, improve appreciation of teamwork, and encourage better communication among health

  16. Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Tuo [Iowa State Univ., Ames, IA (United States); Park, Yong Bum [Pennsylvania State Univ., State College, PA (United States); Caporini, Marc A. [Bruker Biospin Corporation, Billerica, MA (United States); Rosay, Melanie [Bruker Biospin Corporation, Billerica, MA (United States); Zhong, Linghao [Pennsylvania State Univ., State College, PA (United States); Cosgrove, Daniel J. [Pennsylvania State Univ., State College, PA (United States); Hong, Mei [Iowa State Univ., Ames, IA (United States)

    2013-08-29

    Structure determination of protein binding to noncrystalline macromolecular assemblies such as plant cell walls (CWs) poses a significant structural biology challenge. CWs are loosened during growth by expansin proteins, which weaken the noncovalent network formed by cellulose, hemicellulose, and pectins, but the CW target of expansins has remained elusive because of the minute amount of the protein required for activity and the complex nature of the CW. Using solid-state NMR spectroscopy, combined with sensitivity-enhancing dynamic nuclear polarization (DNP) and differential isotopic labeling of expansin and polysaccharides, we have now determined the functional binding target of expansin in the Arabidopsis thaliana CW. By transferring the electron polarization of a biradical dopant to the nuclei, DNP allowed selective detection of 13C spin diffusion from trace concentrations of 13C, 15N-labeled expansin in the CW to nearby polysaccharides. From the spin diffusion data of wild-type and mutant expansins, we conclude that to loosen the CW, expansin binds highly specific cellulose domains enriched in xyloglucan, whereas more abundant binding to pectins is unrelated to activity. Molecular dynamics simulations indicate short 13C-13C distances of 4–6 Å between a hydrophobic surface of the cellulose microfibril and an aromatic motif on the expansin surface, consistent with the observed NMR signals. DNP-enhanced 2D 13C correlation spectra further reveal that the expansin-bound cellulose has altered conformation and is enriched in xyloglucan, thus providing unique insight into the mechanism of CW loosening. DNP-enhanced NMR provides a powerful, generalizable approach for investigating protein binding to complex macromolecular targets.

  17. A Secure, Intelligent, and Smart-Sensing Approach for Industrial System Automation and Transmission over Unsecured Wireless Networks.

    Science.gov (United States)

    Shahzad, Aamir; Lee, Malrey; Xiong, Neal Naixue; Jeong, Gisung; Lee, Young-Keun; Choi, Jae-Young; Mahesar, Abdul Wheed; Ahmad, Iftikhar

    2016-03-03

    In Industrial systems, Supervisory control and data acquisition (SCADA) system, the pseudo-transport layer of the distributed network protocol (DNP3) performs the functions of the transport layer and network layer of the open systems interconnection (OSI) model. This study used a simulation design of water pumping system, in-which the network nodes are directly and wirelessly connected with sensors, and are monitored by the main controller, as part of the wireless SCADA system. This study also intends to focus on the security issues inherent in the pseudo-transport layer of the DNP3 protocol. During disassembly and reassembling processes, the pseudo-transport layer keeps track of the bytes sequence. However, no mechanism is available that can verify the message or maintain the integrity of the bytes in the bytes received/transmitted from/to the data link layer or in the send/respond from the main controller/sensors. To properly and sequentially keep track of the bytes, a mechanism is required that can perform verification while bytes are received/transmitted from/to the lower layer of the DNP3 protocol or the send/respond to/from field sensors. For security and byte verification purposes, a mechanism needs to be proposed for the pseudo-transport layer, by employing cryptography algorithm. A dynamic choice security buffer (SB) is designed and employed during the security development. To achieve the desired goals of the proposed study, a pseudo-transport layer stack model is designed using the DNP3 protocol open library and the security is deployed and tested, without changing the original design.

  18. Design and Analysis of Steerable ECRH Launcher for SST-1 Tokamak

    Science.gov (United States)

    Mistry, Hardik; Shukla, B. K.

    2017-07-01

    In the tokamaks ECRH system is used for pre-ionization, start up, heating, current drive and suppression of NTMs (Neo Classical Tearing Modes). A Standard ECRH system consists of high power microwave source Gyrotron, circular corrugated waveguide based transmission line and launcher. The Focused ECH power is launched into plasma through launcher. The microwave beam emerges out from circular corrugated waveguide and propagates freely in air with finite divergence. So focusing and plane mirror combination is used to launch focused beam in plasma. Thus an ECRH launcher consists of metallic profiled and plane mirror, UHV compatible vacuum barrier window and a UHV gate valve. One 42 GHz gyrotron capable of delivering 500 kW of power for 500 ms and other 82 GHz gyrotron capable of delivering 200 kW of power for 1000s are used for SST-1 ECRH system. The launcher design consists of mirror design, design of supports and design of steering mechanism to provide suitable movements with minimum backless error. The whole assembly is UHV compatible. The launcher is capable of steering the beams by ±20° in both toroidal and poloidal directions. Mirrors are given motion by means of one rotary and one linear feedthrough. For 82 GHz launcher active cooling is provided, whereas for 42 GHz launcher no active cooling is provided. A detailed analysis is carried out for the mirrors of the high power launcher. The heat load for the 82 GHz launcher is 2 kW ( 1% absorption) and for 42 GHz launcher it is 5 kW. For 82 GHz launcher, the maximum steady state surface temperatures of focusing and reflecting mirrors are 315K and 323K and von-mises stresses are within 10 MPa. Similarly for 42 GHz launcher maximum temperatures observed during 500 ms pulse are 301K and 303K for focusing and reflecting mirrors respectively. This paper explains the mechanical and thermal design and analysis of the launcher for the ECRH system.

  19. Theory for cross effect dynamic nuclear polarization under magic-angle spinning in solid state nuclear magnetic resonance: the importance of level crossings.

    Science.gov (United States)

    Thurber, Kent R; Tycko, Robert

    2012-08-28

    We present theoretical calculations of dynamic nuclear polarization (DNP) due to the cross effect in nuclear magnetic resonance under magic-angle spinning (MAS). Using a three-spin model (two electrons and one nucleus), cross effect DNP with MAS for electron spins with a large g-anisotropy can be seen as a series of spin transitions at avoided crossings of the energy levels, with varying degrees of adiabaticity. If the electron spin-lattice relaxation time T(1e) is large relative to the MAS rotation period, the cross effect can happen as two separate events: (i) partial saturation of one electron spin by the applied microwaves as one electron spin resonance (ESR) frequency crosses the microwave frequency and (ii) flip of all three spins, when the difference of the two ESR frequencies crosses the nuclear frequency, which transfers polarization to the nuclear spin if the two electron spins have different polarizations. In addition, adiabatic level crossings at which the two ESR frequencies become equal serve to maintain non-uniform saturation across the ESR line. We present analytical results based on the Landau-Zener theory of adiabatic transitions, as well as numerical quantum mechanical calculations for the evolution of the time-dependent three-spin system. These calculations provide insight into the dependence of cross effect DNP on various experimental parameters, including MAS frequency, microwave field strength, spin relaxation rates, hyperfine and electron-electron dipole coupling strengths, and the nature of the biradical dopants.

  20. Exciton mediated dynamic nuclear polarization in an individual self-assembled quantum dot

    Energy Technology Data Exchange (ETDEWEB)

    Klotz, Florian; Jovanov, Vase; Kierig, Johannes; Clark, Emily C.; Rudolph, Daniel; Heiss, Dominik; Bichler, Max; Abstreiter, Gerhard; Brandt, Martin S.; Finley, Jonathan J. [Walter Schottky Institut, Garching (Germany)

    2010-07-01

    We present investigations of dynamic nuclear polarization (DNP) in a single self-assembled InGaAs/GaAs quantum dot subject to an external magnetic field (B{sub 0}) and electric field parallel to B{sub 0}. Resonant excitation of the neutral exciton (X{sup 0}) is shown to lead to a buildup of nuclear spin orientation due to tunneling escape of photoexcited carriers that serves to recycle dark states formed by hyperfine-mediated electron-nuclear spin flip-flop processes. DNP was achieved by tuning one Zeeman level of X{sup 0} into resonance with a single frequency laser to optically pump spin-polarized carriers. We estimated the resulting Overhauser field B{sub N} by locating both Zeeman branches relative to a measurement where DNP was inhibited. The saturation value of B{sub N} depends on whether sweeps are performed from low to high electric fields or vice versa, B{sub 0} and the timescale over which the sweeps are performed. B{sub N} is always found to be oriented parallel to B{sub 0} with magnitude B{sub N}{proportional_to}3-5 T. In addition, we also performed time resolved measurements of the nuclear spin orientation dynamics and found B{sub N} to increase over timescales ranging from a few seconds to several minutes depending on the excitation intensity, electric field and detuning from resonance.

  1. 依帕司他治疗糖尿病周围神经病变疗效观察%The therapeutic effect of Epalrestat on diabetic peripheral neuropathy

    Institute of Scientific and Technical Information of China (English)

    贺颖

    2013-01-01

    目的观察依帕司他治疗糖尿病周围神经病变(DNP)的疗效。方法 DNP患者75例随机分为:治疗组39例,对照组36例。对照组给予甲钴胺0.5mg静脉推注,治疗组在对照组基础上加用依帕司他50mg,日3次口服,疗程12周。结果治疗组临床疗效、神经传导速度改善明显优于对照组,差异有统计学意义(PDNP. Methods 75 DNP patients were randomly divided into two groups:the treatment group with Eepalrestat and mecobalamin(n=39),the control group with mecobalamin (n=36). Each treatment lasted for twelve weeks.Results Treatment group versus control group improved nerve conduction velocity, and also relieved clinical symptoms(P<0.05). Conclusion Eepalrestat and mecobalamin could accelerate the nerve conduction velocity and has a significant effect on DNP.

  2. Design and Development of Layered Security: Future Enhancements and Directions in Transmission

    Directory of Open Access Journals (Sweden)

    Aamir Shahzad

    2016-01-01

    Full Text Available Today, security is a prominent issue when any type of communication is being undertaken. Like traditional networks, supervisory control and data acquisition (SCADA systems suffer from a number of vulnerabilities. Numerous end-to-end security mechanisms have been proposed for the resolution of SCADA-system security issues, but due to insecure real-time protocol use and the reliance upon open protocols during Internet-based communication, these SCADA systems can still be compromised by security challenges. This study reviews the security challenges and issues that are commonly raised during SCADA/protocol transmissions and proposes a secure distributed-network protocol version 3 (DNP3 design, and the implementation of the security solution using a cryptography mechanism. Due to the insecurities found within SCADA protocols, the new development consists of a DNP3 protocol that has been designed as a part of the SCADA system, and the cryptographically derived security is deployed within the application layer as a part of the DNP3 stack.

  3. Suppressive effects of anti-allergic agent suplatast tosilate (IPD-1151T on the expression of co-stimulatory molecules on mouse splenocytes in vivo

    Directory of Open Access Journals (Sweden)

    Masatsugu Kurokawa

    2001-01-01

    Full Text Available The effects of IPD-1151T on the expression of costimulatory molecules, CD40, CD80 and CD86, were investigated in vivo using mice with allergic disorders. BALB/c mice were immunized intraperitoneally with two doses of dinitrophenylated ovalbumin (DNP-OVA at 1-week intervals. These mice then were treated intraperitoneally with 100μg/kg of IPD1151T once a day for 14 days, starting 7 days after the first immunization. On day 21, some mice were challenged intraperitoneally with DNP-OVA and the other mice were not challenged. All mice were autopsied on day 22 and assayed for immunoglobulin E, interleuken (IL-4 and IL-5 productions following DNP-OVA immunization. The intraperitoneal treatment with IPD-1151T strongly suppressed immunoglobulin E contents in serum, which were enhanced by DNA-OVA immunization. IPD-1151T also caused a decrease in both IL-4 and IL-5 levels in splenic lymphocytes. We next examined the influence of IPD1151T on co-stimulatory molecule expression on splenic lymphocytes. IPD-1151T caused suppression of CD40 and CD86 expression; however, the treatments did not affect CD80 expression.

  4. Thermosetting polymer for dynamic nuclear polarization: Solidification of an epoxy resin mixture including TEMPO

    Energy Technology Data Exchange (ETDEWEB)

    Noda, Yohei, E-mail: noda.yohei@jaea.go.jp [Quantum Beam Science Centre, Sector of Nuclear Science Research, Japan Atomic Energy Agency, Shirakata-Shirane, Tokai, Ibaraki 319-1195 (Japan); Kumada, Takayuki [Quantum Beam Science Centre, Sector of Nuclear Science Research, Kansai Photon Science Institute, Japan Atomic Energy Agency, Kizugawa, Kyoto 619-0215 (Japan); Yamaguchi, Daisuke; Shamoto, Shin-ichi [Quantum Beam Science Centre, Sector of Nuclear Science Research, Japan Atomic Energy Agency, Shirakata-Shirane, Tokai, Ibaraki 319-1195 (Japan)

    2015-03-11

    We investigated the dynamic nuclear polarization (DNP) of typical thermosetting polymers (two-component type epoxy resins; Araldite{sup ®} Standard or Araldite{sup ®} Rapid) doped with a (2,2,6,6-tetramethylpiperidine-1-yl)oxy (TEMPO) radical. The doping process was developed by carefully considering the decomposition of TEMPO during the solidification of the epoxy resin. The TEMPO electron spin in each two-component paste decayed slowly, which was favorable for our study. Furthermore, despite the dissolved TEMPO, the mixture of the two-component paste successfully solidified. With the resulting TEMPO-doped epoxy-resin samples, DNP experiments at 1.2 K and 3.35 T indicated a magnitude of a proton-spin polarization up to 39%. This polarization is similar to that (35%) obtained for TEMPO-doped polystyrene (PS), which is often used as a standard sample for DNP. To combine this solidification of TEMPO-including mixture with a resin-casting technique enables a creation of polymeric target materials with a precise and complex structure.

  5. Suppression of gyrase-mediated resistance by C7 aryl fluoroquinolones

    Science.gov (United States)

    Malik, Muhammad; Mustaev, Arkady; Schwanz, Heidi A.; Luan, Gan; Shah, Nirali; Oppegard, Lisa M.; de Souza, Ernane C.; Hiasa, Hiroshi; Zhao, Xilin; Kerns, Robert J.; Drlica, Karl

    2016-01-01

    Fluoroquinolones form drug-topoisomerase-DNA complexes that rapidly block transcription and replication. Crystallographic and biochemical studies show that quinolone binding involves a water/metal-ion bridge between the quinolone C3-C4 keto-acid and amino acids in helix-4 of the target proteins, GyrA (gyrase) and ParC (topoisomerase IV). A recent cross-linking study revealed a second drug-binding mode in which the other end of the quinolone, the C7 ring system, interacts with GyrA. We report that addition of a dinitrophenyl (DNP) moiety to the C7 end of ciprofloxacin (Cip-DNP) reduced protection due to resistance substitutions in Escherichia coli GyrA helix-4, consistent with the existence of a second drug-binding mode not evident in X-ray structures of drug-topoisomerase-DNA complexes. Several other C7 aryl fluoroquinolones behaved in a similar manner with particular GyrA mutants. Treatment of E. coli cultures with Cip-DNP selectively enriched an uncommon variant, GyrA-A119E, a change that may impede binding of the dinitrophenyl group at or near the GyrA-GyrA interface. Collectively the data support the existence of a secondary quinolone-binding mode in which the quinolone C7 ring system interacts with GyrA; the data also identify C7 aryl derivatives as a new way to obtain fluoroquinolones that overcome existing GyrA-mediated quinolone resistance. PMID:26984528

  6. Low-temperature dynamic nuclear polarization with helium-cooled samples and nitrogen-driven magic-angle spinning.

    Science.gov (United States)

    Thurber, Kent; Tycko, Robert

    2016-03-01

    We describe novel instrumentation for low-temperature solid state nuclear magnetic resonance (NMR) with dynamic nuclear polarization (DNP) and magic-angle spinning (MAS), focusing on aspects of this instrumentation that have not been described in detail in previous publications. We characterize the performance of an extended interaction oscillator (EIO) microwave source, operating near 264 GHz with 1.5 W output power, which we use in conjunction with a quasi-optical microwave polarizing system and a MAS NMR probe that employs liquid helium for sample cooling and nitrogen gas for sample spinning. Enhancement factors for cross-polarized (13)C NMR signals in the 100-200 range are demonstrated with DNP at 25K. The dependences of signal amplitudes on sample temperature, as well as microwave power, polarization, and frequency, are presented. We show that sample temperatures below 30K can be achieved with helium consumption rates below 1.3 l/h. To illustrate potential applications of this instrumentation in structural studies of biochemical systems, we compare results from low-temperature DNP experiments on a calmodulin-binding peptide in its free and bound states.

  7. Dynamic nuclear polarization at 40 kHz magic angle spinning.

    Science.gov (United States)

    Chaudhari, Sachin R; Berruyer, Pierrick; Gajan, David; Reiter, Christian; Engelke, Frank; Silverio, Daniel L; Copéret, Christophe; Lelli, Moreno; Lesage, Anne; Emsley, Lyndon

    2016-04-21

    DNP-enhanced solid-state NMR spectroscopy under magic angle spinning (MAS) is rapidly developing into a powerful analytical tool to investigate the structure of a wide range of solid materials, because it provides unsurpassed sensitivity gains. Most developments and applications of DNP MAS NMR were so far reported at moderate spinning frequencies (up to 14 kHz using 3.2 mm rotors). Here, using a 1.3 mm MAS DNP probe operating at 18.8 T and ∼100 K, we show that signal amplification factors can be increased by up to a factor two when using smaller volume rotors as compared to 3.2 mm rotors, and report enhancements of around 60 over a range of sample spinning rates from 10 to 40 kHz. Spinning at 40 kHz is also shown to increase (29)Si coherence lifetimes by a factor three as compared to 10 kHz, substantially increasing sensitivity in CPMG type experiments. The contribution of quenching effects to the overall sensitivity gain at very fast MAS is evaluated, and applications are reported on a functionalised mesostructured organic-inorganic material.

  8. Randomized trial of two schedules of low-dose gemtuzumab ozogamicin as induction monotherapy for newly diagnosed acute myeloid leukemia in older patients not considered candidates for intensive chemotherapy. A phase II study of the EORTC and GIMEMA leukemia groups (AML-19)

    Science.gov (United States)

    Amadori, Sergio; Suciu, Stefan; Selleslag, Dominik; Stasi, Roberto; Alimena, Giuliana; Baila, Liliana; Rizzoli, Vittorio; Borlenghi, Erika; Gaidano, Gianluca; Magro, Domenico; Torelli, Giuseppe; Muus, Petra; Venditti, Adriano; Cacciola, Emma; Lauria, Francesco; Vignetti, Marco; de Witte, Theo

    2010-01-01

    Summary This study compared two schedules of low-dose gemtuzumab ozogamicin (GO) as induction monotherapy for untreated acute myeloid leukemia in older patients unfit for intensive chemotherapy, to identify the more promising regimen for further study. Patients were randomized to receive either best supportive care or a course of GO according to one of two schedules: 3 mg/m2 on days 1, 3 and 5 (arm A), or GO 6 mg/m2 on day 1 and 3 mg/m2 on day 8 (arm B). Primary endpoint was the rate of disease non-progression (DnP), defined as the proportion of patients either achieving a response or maintaining a stable disease following GO induction in each arm. Fifty-six patients were randomized in the two GO arms (A, n=29; B, n=27). The rate of DnP was 38% (90% confidence interval [CI], 23%–55%) in arm A, and 63% (90% CI, 45%–78%) in arm B. Peripheral cytopenias were the most common adverse events for both regimens. The all-cause early mortality rate was 14% in arm A and 11% in arm B. The day 1+8 schedule, which was associated with the highest rate of DnP, met the statistical criteria to be selected as the preferred regimen for phase III comparison with best supportive care. PMID:20230405

  9. Membrane topologies of the PGLa antimicrobial peptide and a transmembrane anchor sequence by Dynamic Nuclear Polarization/solid-state NMR spectroscopy.

    Science.gov (United States)

    Salnikov, Evgeniy Sergeevich; Aisenbrey, Christopher; Aussenac, Fabien; Ouari, Olivier; Sarrouj, Hiba; Reiter, Christian; Tordo, Paul; Engelke, Frank; Bechinger, Burkhard

    2016-02-15

    Dynamic Nuclear Polarization (DNP) has been introduced to overcome the sensitivity limitations of nuclear magnetic resonance (NMR) spectroscopy also of supported lipid bilayers. When investigated by solid-state NMR techniques the approach typically involves doping the samples with biradicals and their investigation at cryo-temperatures. Here we investigated the effects of temperature and membrane hydration on the topology of amphipathic and hydrophobic membrane polypeptides. Although the antimicrobial PGLa peptide in dimyristoyl phospholipids is particularly sensitive to topological alterations, the DNP conditions represent well its membrane alignment also found in bacterial lipids at ambient temperature. With a novel membrane-anchored biradical and purpose-built hardware a 17-fold enhancement in NMR signal intensity is obtained by DNP which is one of the best obtained for a truly static matrix-free system. Furthermore, a membrane anchor sequence encompassing 19 hydrophobic amino acid residues was investigated. Although at cryotemperatures the transmembrane domain adjusts it membrane tilt angle by about 10 degrees, the temperature dependence of two-dimensional separated field spectra show that freezing the motions can have beneficial effects for the structural analysis of this sequence.

  10. Suppression of gyrase-mediated resistance by C7 aryl fluoroquinolones.

    Science.gov (United States)

    Malik, Muhammad; Mustaev, Arkady; Schwanz, Heidi A; Luan, Gan; Shah, Nirali; Oppegard, Lisa M; de Souza, Ernane C; Hiasa, Hiroshi; Zhao, Xilin; Kerns, Robert J; Drlica, Karl

    2016-04-20

    Fluoroquinolones form drug-topoisomerase-DNA complexes that rapidly block transcription and replication. Crystallographic and biochemical studies show that quinolone binding involves a water/metal-ion bridge between the quinolone C3-C4 keto-acid and amino acids in helix-4 of the target proteins, GyrA (gyrase) and ParC (topoisomerase IV). A recent cross-linking study revealed a second drug-binding mode in which the other end of the quinolone, the C7 ring system, interacts with GyrA. We report that addition of a dinitrophenyl (DNP) moiety to the C7 end of ciprofloxacin (Cip-DNP) reduced protection due to resistance substitutions in Escherichia coli GyrA helix-4, consistent with the existence of a second drug-binding mode not evident in X-ray structures of drug-topoisomerase-DNA complexes. Several other C7 aryl fluoroquinolones behaved in a similar manner with particular GyrA mutants. Treatment of E. coli cultures with Cip-DNP selectively enriched an uncommon variant, GyrA-A119E, a change that may impede binding of the dinitrophenyl group at or near the GyrA-GyrA interface. Collectively the data support the existence of a secondary quinolone-binding mode in which the quinolone C7 ring system interacts with GyrA; the data also identify C7 aryl derivatives as a new way to obtain fluoroquinolones that overcome existing GyrA-mediated quinolone resistance.

  11. Randomized trial of two schedules of low-dose gemtuzumab ozogamicin as induction monotherapy for newly diagnosed acute myeloid leukaemia in older patients not considered candidates for intensive chemotherapy. A phase II study of the EORTC and GIMEMA leukaemia groups (AML-19).

    Science.gov (United States)

    Amadori, Sergio; Suciu, Stefan; Selleslag, Dominik; Stasi, Roberto; Alimena, Giuliana; Baila, Liliana; Rizzoli, Vittorio; Borlenghi, Erika; Gaidano, Gianluca; Magro, Domenico; Torelli, Giuseppe; Muus, Petra; Venditti, Adriano; Cacciola, Emma; Lauria, Francesco; Vignetti, Marco; de Witte, Theo

    2010-05-01

    This study compared two schedules of low-dose gemtuzumab ozogamicin (GO) as induction monotherapy for untreated acute myeloid leukaemia in older patients unfit for intensive chemotherapy, to identify the more promising regimen for further study. Patients were randomized to receive either best supportive care or a course of GO according to one of two schedules: 3 mg/m(2) on days 1, 3 and 5 (arm A), or GO 6 mg/m(2) on day 1 and 3 mg/m(2) on day 8 (arm B). Primary endpoint was the rate of disease non-progression (DnP), defined as the proportion of patients either achieving a response or maintaining a stable disease following GO induction in each arm. Fifty-six patients were randomized in the two GO arms (A, n = 29; B, n = 27). The rate of DnP was 38% [90% confidence interval (CI), 23-55] in arm A, and 63% (90% CI, 45-78) in arm B. Peripheral cytopenias were the most common adverse events for both regimens. The all-cause early mortality rate was 14% in arm A and 11% in arm B. The day 1 + 8 schedule, which was associated with the highest rate of DnP, met the statistical criteria to be selected as the preferred regimen for phase III comparison with best supportive care.

  12. The impact of floc size on respiration inhibition by soluble toxicants--a comparative investigation.

    Science.gov (United States)

    Henriques, Inês D S; Holbrook, R David; Kelly, Richard T; Love, Nancy G

    2005-07-01

    Activated sludge facilities are susceptible to upset by shock loads of toxic compounds. We hypothesized that floc size plays an important role in determining the sensitivity of mixed liquor to shock by cadmium and 2,4-dinitrophenol (DNP). To test this hypothesis, heterotrophic respiration inhibition experiments were conducted using mixed liquor from a pilot-scale membrane bioreactor (MBR) and full-scale activated sludge (FSAS) facility with gravity settling secondary clarifiers that were operated under similar process conditions. MBR mixed liquor flocs were both 41% smaller and 2 and 1.25 times more sensitive to equivalent soluble cadmium and DNP concentrations, respectively, compared to FSAS mixed liquor flocs. Similarly, FSAS mixed liquor that had been sheared (resulting in a smaller average floc diameter) was 1.5 times more sensitive to soluble cadmium than non-sheared FSAS mixed liquor. These results suggest that activated sludge process conditions that create smaller floc particles, such as the use of membranes for liquid-solid separation, are more susceptible to upset events caused by shock loads of cadmium and DNP. The particle size distribution (PSD) and average floc diameter of a mixed liquor suspension should be measured and reported when stating the inhibition concentration of a specific toxicant.

  13. Development of a Wireless Love Wave Biosensor Platform for Multi-functional Detection

    Science.gov (United States)

    Song, Taehyeon; Song, Seung Yeon; Yoon, Hyun C.; Lee, Keekeun

    2011-06-01

    A Love wave-based biosensor with a 440 MHz operating frequency was developed for simultaneous detection of two different concentrations of anti-dinitrophenyl-keyhole limpet hemocyanin (Anti-DNP-KLH) rabbit immunoglobulin G (IgG) in a single sensor. The sensor was composed of surface acoustic wave (SAW) reflective delay lines built from interdigital transducer (IDT) and several reflectors, a poly(methyl methacrylate) (PMMA) waveguide layer, and two sensitive films. To extract optimal device parameters, coupling of mode (COM) modeling was carried. According to the device parameters determined, the Love wave biosensor was fabricated and then wirelessly characterized by a network analyzer. Binding of anti-DNP IgG to DNP induced a change in the time positions of the original reflection peaks mainly due to the mass loading effect. The measured time positions were matched well with the predicted values from COM modeling. The sensitivities evaluated from the first and second sensitive films were 167.9 and 44.8 deg·µg-1·ml-1, respectively.

  14. Experimental design-guided development of a stereospecific capillary electrophoresis assay for methionine sulfoxide reductase enzymes using a diastereomeric pentapeptide substrate.

    Science.gov (United States)

    Zhu, Qingfu; Huo, Xingyu; Heinemann, Stefan H; Schönherr, Roland; El-Mergawy, Rabab; Scriba, Gerhard K E

    2014-09-12

    A capillary electrophoresis method has been developed and validated to evaluate the stereospecific activity of recombinant human methionine sulfoxide reductase enzymes employing the C-terminally dinitrophenyl-labeled N-acetylated pentapeptide ac-KIFM(O)K-Dnp as substrate (M(O)=methionine sulfoxide). The separation of the ac-KIFM(O)K-Dnp diastereomers and the reduced peptide ac-KIFMK-Dnp was optimized using experimental design with regard to the buffer pH, buffer concentration, sulfated β-cyclodextrin and 15-crown-5 concentration as well as capillary temperature and separation voltage. A fractional factorial response IV design was employed for the identification of the significant factors and a five-level circumscribed central composite design for the final method optimization. Resolution of the peptide diastereomers as well as analyte migration time served as responses in both designs. The resulting optimized conditions included 50mM Tris buffer, pH 7.85, containing 5mM 15-crown-5 and 14.3mg/mL sulfated β-cyclodextrin, at an applied voltage of 25kV and a capillary temperature of 21.5°C. The assay was subsequently applied to the determination of the stereospecificity of recombinant human methionine sulfoxide reductases A and B2. The Michaelis-Menten kinetic data were determined. The pentapeptide proved to be a good substrate for both enzymes. Furthermore, the first separation of methionine sulfoxide peptide diastereomers is reported.

  15. Design of angiotensin II derivatives suitable for indirect affinity techniques: potential applications to receptor studies.

    Science.gov (United States)

    Bonnafous, J C; Seyer, R; Tence, M; Marie, J; Kabbaj, M; Aumelas, A

    1988-01-01

    The design of angiotensin II (A II)-derived probes suitable for indirect affinity techniques is presented. Biotin or dinitrophenyl moieties have been added at the N-terminus of A II, through aminohexanoic acid as spacer arm, to generate (6-biotinylamido)-hexanoyl-AII (Bio-Ahx-AII) and dinitrophenyl- aminohexanoyl-AII (Dnp-Ahx-AII). Monoiodinated and highly labeled radioiodinated forms of these probes have been prepared. The two bifunctional ligands displayed high affinities for rat liver A II receptors (Kd values in the nanomolar range) and their secondary acceptors: streptavidin and monoclonal anti-Dnp antibodies respectively. Bio-Ahx-AII and Dnp-Ahx-AII behaved as agonists on several AII-sensitive systems. Based on these structural assessments, the parent photoactivable azido probe: Bio-Ahx-(Ala1,Phe(4N3)8)A II. A II was synthesized and proved to possess similar biological properties than the non-azido compound. The hepatic A II receptor could be covalently labeled by the radioiodinated probe, with a particularly high yield (15-20%); SDS-polyacrylamide gel electrophoresis of solubilized complexes revealed specific labeling of a 65 Kdaltons binding unit, in agreement with previous data obtained with other azido AII-derived compounds. The potential applications of these probes are: i) receptor purification by combination of its photoaffinity labeling and adsorption of biotin-tagged solubilized hormone-receptor complexes on avidin gels. ii) cell labeling and sorting. iii) histochemical receptor visualization.

  16. Characterizing the interactions of organic nanoparticles with renal epithelial cells in vivo.

    Science.gov (United States)

    Nair, Anil V; Keliher, Edmund J; Core, Amanda B; Brown, Dennis; Weissleder, Ralph

    2015-01-01

    Nanotechnology approaches are actively being pursued for drug delivery, novel diagnostics, implantable devices, and consumer products. While considerable research has been performed on the effects of these materials on targeted tumor or phagocytic cells, relatively little is known about their effects on renal cells. This becomes critical for supersmall nanoparticles (nanoparticles. To test whether such interactions affect kidney function, we injected mice with either 5 nm dextran-based nanoparticles (DNP) that are similar in composition to FDA-approved materials or poly(amido amine) dendrimer nanoparticles (PNP) of comparable size. These fluorescently tagged nanoparticles were both filtered and internalized by renal tubular epithelial cells in a dose- and time-dependent fashion. The biological effects were quantitated by immunocytochemistry, measuring kidney injury markers and performing functional tests. DNP administration resulted in a dose-dependent increase in urinary output, while cellular albumin endocytosis was increased. The expression of megalin, a receptor involved in albumin uptake, was also increased, but AQP1 expression was unaffected. The effects after PNP administration were similar but additionally resulted in increased clathrin expression and increased endocytosis of dextran. We conclude that there are no major detrimental renal effects of DNP on overall kidney function, but changes in endocytosis-mediating protein expression do occur. These studies provide a framework for the testing of additional nanoparticle preparations as they become available.

  17. New Security Development and Trends to Secure the SCADA Sensors Automated Transmission during Critical Sessions

    Directory of Open Access Journals (Sweden)

    Aamir Shahzad

    2015-10-01

    Full Text Available Modern technology enhancements have been used worldwide to fulfill the requirements of the industrial sector, especially in supervisory control and data acquisition (SCADA systems as a part of industrial control systems (ICS. SCADA systems have gained popularity in industrial automations due to technology enhancements and connectivity with modern computer networks and/or protocols. The procurement of new technologies has made SCADA systems important and helpful to processing in oil lines, water treatment plants, and electricity generation and control stations. On the other hand, these systems have vulnerabilities like other traditional computer networks (or systems, especially when interconnected with open platforms. Many international organizations and researchers have proposed and deployed solutions for SCADA security enhancement, but most of these have been based on node-to-node security, without emphasizing critical sessions that are linked directly with industrial processing and automation. This study concerns SCADA security measures related to critical processing with specified sessions of automated polling, analyzing cryptography mechanisms and deploying the appropriate explicit inclusive security solution in a distributed network protocol version 3 (DNP3 stack, as part of a SCADA system. The bytes flow through the DNP3 stack with security computational bytes within specified critical intervals defined for polling. We took critical processing knowledge into account when designing a SCADA/DNP3 testbed and deploying a cryptography solution that did not affect communications.

  18. Low-temperature dynamic nuclear polarization with helium-cooled samples and nitrogen-driven magic-angle spinning

    Science.gov (United States)

    Thurber, Kent; Tycko, Robert

    2016-03-01

    We describe novel instrumentation for low-temperature solid state nuclear magnetic resonance (NMR) with dynamic nuclear polarization (DNP) and magic-angle spinning (MAS), focusing on aspects of this instrumentation that have not been described in detail in previous publications. We characterize the performance of an extended interaction oscillator (EIO) microwave source, operating near 264 GHz with 1.5 W output power, which we use in conjunction with a quasi-optical microwave polarizing system and a MAS NMR probe that employs liquid helium for sample cooling and nitrogen gas for sample spinning. Enhancement factors for cross-polarized 13C NMR signals in the 100-200 range are demonstrated with DNP at 25 K. The dependences of signal amplitudes on sample temperature, as well as microwave power, polarization, and frequency, are presented. We show that sample temperatures below 30 K can be achieved with helium consumption rates below 1.3 l/h. To illustrate potential applications of this instrumentation in structural studies of biochemical systems, we compare results from low-temperature DNP experiments on a calmodulin-binding peptide in its free and bound states.

  19. Dynamic Nuclear Polarization Fast Field Cycling Method for the Selective Study of Molecular Dynamics in Block Copolymers.

    Science.gov (United States)

    Gizatullin, Bulat; Neudert, Oliver; Stapf, Siegfried; Mattea, Carlos

    2017-09-06

    Dynamic nuclear polarization (DNP) is one of the most useful methods to increase sensitivity in NMR spectroscopy. It is based on the transfer of magnetization from an electron to the nuclear spin system. Based on previous work that demonstrated the feasibility of integrating DNP with fast field cycling (FFC) relaxometry and the possibility to distinguish between different mechanisms, such as the Overhauser effect (OE) and the solid effect (SE), the first FFC study of the differential relaxation properties of a copolymer is presented. For this purpose, concentrated solutions of the polystyrene-block-polybutadiene-block-polystyrene (SBS) triblock copolymer and the corresponding homopolymers were investigated. T1 -T2 relaxation data are discussed in terms of molecular mobility and the presence of radicals. The DNP selective data indicate a dominant SE contribution to the enhancement of the NMR signal for both blocks of the triblock copolymer and for the homopolymer solutions. The enhancement factors are different for both polymer types and in the copolymer, which is explained by the individual (1) H T1 relaxation times and different electron-nucleus coupling strength. The T1 relaxation dispersion measurements of the SE enhanced signal were performed, which led to improved signal-to-noise ratios that allowed the site-specific separation of relaxation times and their dependence on the Larmor frequency with a higher accuracy. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Design and Development of Layered Security: Future Enhancements and Directions in Transmission.

    Science.gov (United States)

    Shahzad, Aamir; Lee, Malrey; Kim, Suntae; Kim, Kangmin; Choi, Jae-Young; Cho, Younghwa; Lee, Keun-Kwang

    2016-01-06

    Today, security is a prominent issue when any type of communication is being undertaken. Like traditional networks, supervisory control and data acquisition (SCADA) systems suffer from a number of vulnerabilities. Numerous end-to-end security mechanisms have been proposed for the resolution of SCADA-system security issues, but due to insecure real-time protocol use and the reliance upon open protocols during Internet-based communication, these SCADA systems can still be compromised by security challenges. This study reviews the security challenges and issues that are commonly raised during SCADA/protocol transmissions and proposes a secure distributed-network protocol version 3 (DNP3) design, and the implementation of the security solution using a cryptography mechanism. Due to the insecurities found within SCADA protocols, the new development consists of a DNP3 protocol that has been designed as a part of the SCADA system, and the cryptographically derived security is deployed within the application layer as a part of the DNP3 stack.