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Sample records for 2-hour urokinase regime

  1. Efficacy and safety of 2-hour urokinase regime in acute pulmonary embolism: a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Huang Kewu

    2009-12-01

    Full Text Available Abstract Backgrounds Urokinase (UK 2 200 U/kg·h for 12 hours infusion(UK-12 his an ACCP recommended regimen in treating acute pulmonary embolism (PE. It is unclear whether this dose and time can be reduced further. We compared the efficacy and safety of 20, 000 U/kg for 2 hours (UK-2 h with the UK-12 h regime in selected PE patients. Methods A randomized trial involving 129 patients was conducted. Patients with acute PE were randomly assigned to receive either UK-12 h (n = 70, or UK-2 h (n = 59. The efficacy was determined by the improvement of right heart dysfunction and perfusion defect at 24 h and 14 d post UK treatment. The bleeding incidence, death rate and PE recurrence were also evaluated. Results Similarly significant improvements in right heart dysfunction and lung perfusion defects were observed in both groups. Overall bleeding incidents were low in both groups. Major bleeding directly associated with UK infusion occurred in one patient in the UK-2 h group and one in the UK-12 h group. Mortality rates were low, with one reported fatal recurrent in the UK-12 h group and none in the UK-2 h group. When the rate of bleeding, death and PE recurrence were compared separately in the hemodynamic instability and the massive anatomic obstruction subgroups, no significant difference was found. Conclusions The UK-2 h regimen exhibits similar efficacy and safety as the UK-12 h regimen for acute PE. Trial Registration Clinical trial registered with http://clinicaltrials.gov/ct2/show/NCT00799968 (Identifier: NCT 00799968

  2. Molecular-specific urokinase antibodies

    Science.gov (United States)

    Atassi, M. Zouhair (Inventor); Morrison, Dennis R. (Inventor)

    2009-01-01

    Antibodies have been developed against the different molecular forms of urokinase using synthetic peptides as immunogens. The peptides were synthesized specifically to represent those regions of the urokinase molecules which are exposed in the three-dimensional configuration of the molecule and are uniquely homologous to urokinase. Antibodies are directed against the lysine 158-isoleucine 159 peptide bond which is cleaved during activation from the single-chain (ScuPA) form to the bioactive double chain (54 KDa and 33 KDa) forms of urokinase and against the lysine 135 lysine 136 bond that is cleaved in the process of removing the alpha-chain from the 54 KDa form to produce the 33 KDa form of urokinase. These antibodies enable the direct measurement of the different molecular forms of urokinase from small samples of conditioned medium harvested from cell cultures.

  3. Thrombolytic treatment for acute ischemic cerebral stroke: intraarterial urokinase infusion vs. intravenous heparin and urokinase infusion

    International Nuclear Information System (INIS)

    To evaluate the efficacy and limitation of intra-arterial urokinase (IAUK) infusion for treatment of acute cerebral stroke. Twenty-seven acute cerebral stroke patients treated with IAUK infusion within six hours of stroke onset were reviewed. All patients showed normal initial brain findings on CT. In 21 patients, urokinase(5-15 x 105IU) was administered through a microcatheter placed into or proximal to occluded segment. Mechanical disruption of thrombus by guidewire was performed in 17 patients. Angiographic and clinical responses and complications after IAUK infusion, were evaluated and the results were compared with those of intravenous heparin(N=19) and urokinase infusion(N=19). Complete or partial angiographic recanalization of occluded segment was found in 18 patients (67%), and neurologic improvement was followed in 14 patients(52%). The degree of improvement on the stroke scale score after IAUK infusion was statistically more significant(p<0.05) than that shown after intravenous heparin and urokinase infusion. Complications after IAUK infusion were large(15%) and small amount intracerebral hemorrhage(15%), contrast leakage into brain parenchyma(11%), and gastrointestinal bleeding(4%). Between the IAVK and the intravenous urokinase infusion group, differences in extent and types of complications were statistically insignificant, but were significantly higher in those two groups than in the intravenous heparin infusion group. IAUK infusion may be effective for the treatment of acute cerebral stroke

  4. Urokinase-catalysed cleavage of the urokinase receptor requires an intact glycolipid anchor

    DEFF Research Database (Denmark)

    Høyer-Hansen, G; Pessara, U; Holm, A;

    2001-01-01

    Urokinase (uPA) has the striking ability to cleave its receptor, uPAR, thereby inactivating the binding potential of this molecule. Here we demonstrate that the glycosylphosphatidylinositol (GPI) anchor of uPAR, which is attached to the third domain, is an important determinant in governing this ...

  5. A composite role of vitronectin and urokinase in the modulation of cell morphology upon expression of the urokinase receptor

    DEFF Research Database (Denmark)

    Hillig, Thore; Engelholm, Lars H; Ingvarsen, Signe;

    2008-01-01

    The urokinase receptor, urokinase receptor (uPAR), is a glycosylphosphatidylinositol-anchored membrane protein engaged in pericellular proteolysis and cellular adhesion, migration, and modulation of cell morphology. A direct matrix adhesion is mediated through the binding of uPAR to vitronectin, ...

  6. Using urokinase to restore patency in double lumen catheters.

    Science.gov (United States)

    Northsea, C

    1994-08-01

    All hemodialysis patients with temporary or permanent double lumen catheters are at risk for catheter occlusion. Clinical outcomes and cost-effectiveness of using urokinase, a thrombolytic agent, to declot occluded double lumen dialysis catheters were evaluated for 2 years. Patency was restored in 95 of 102 catheters. These data support the use of urokinase to safely and effectively restore patency, thereby extending the length of time a catheter can be used for dialysis.

  7. The intact urokinase receptor is required for efficient vitronectin binding

    DEFF Research Database (Denmark)

    Høyer-Hansen, G; Behrendt, N; Ploug, M;

    1997-01-01

    The urokinase receptor (uPAR) is a receptor for both urokinase plasminogen activator (uPA) and the adhesion protein vitronectin. There are two forms of cell surface-bound uPAR; intact uPAR and a cleaved form, uPAR(2+3), which is formed by uPA-catalyzed cleavage of uPAR. In ligand-blotting experim...

  8. A urokinase receptor-associated protein with specific collagen binding properties

    DEFF Research Database (Denmark)

    Behrendt, N; Jensen, Ole Nørregaard; Engelholm, L H;

    2000-01-01

    The plasminogen activation cascade system, directed by urokinase and the urokinase receptor, plays a key role in extracellular proteolysis during tissue remodeling. To identify molecular interaction partners of these trigger proteins on the cell, we combined covalent protein cross-linking with mass...... spectrometry based methods for peptide mapping and primary structure analysis of electrophoretically isolated protein conjugates. A specific tri-molecular complex was observed upon addition of pro-urokinase to human U937 cells. This complex included the urokinase receptor, pro-urokinase, and an unknown, high...... molecular weight urokinase receptor-associated protein. The tryptic peptide mixture derived from a cross-linked complex of pro-urokinase and the latter protein was analyzed by nanoelectrospray tandem mass spectrometric sequencing. This analysis identified the novel protein as the human homologue of a murine...

  9. Signaling through urokinase and urokinase receptor in lung cancer cells requires interactions with beta1 integrins.

    Science.gov (United States)

    Tang, Chi-Hui; Hill, Marla L; Brumwell, Alexis N; Chapman, Harold A; Wei, Ying

    2008-11-15

    The urokinase receptor (uPAR) is upregulated upon tumor cell invasion and correlates with poor lung cancer survival. Although a cis-interaction with integrins has been ascribed to uPAR, whether this interaction alone is critical to urokinase (uPA)- and uPAR-dependent signaling and tumor promotion is unclear. Here we report the functional consequences of point mutations of uPAR (H249A-D262A) that eliminate beta1 integrin interactions but maintain uPA binding, vitronectin attachment and association with alphaV integrins, caveolin and epidermal growth factor receptor. Disruption of uPAR interactions with beta1 integrins recapitulated previously reported findings with beta1-integrin-derived peptides that attenuated matrix-dependent ERK activation, MMP expression and in vitro migration by human lung adenocarcinoma cell lines. The uPAR mutant cells acquired enhanced capacity to adhere to vitronectin via uPAR-alphaVbeta5-integrin, rather than through the uPAR-alpha3beta1-integrin complex and they were unable to initiate uPA signaling to activate ERK, Akt or Stat1. In an orthotopic lung cancer model, uPAR mutant cells exhibited reduced tumor size compared with cells expressing wild-type uPAR. Taken together, the results indicate that uPAR-beta1-integrin interactions are essential to signals induced by integrin matrix ligands or uPA that support lung cancer cell invasion in vitro and progression in vivo. PMID:18940913

  10. Bicyclic peptide inhibitor of urokinase-type plasminogen activator

    DEFF Research Database (Denmark)

    Roodbeen, Renée; Jensen, Berit Paaske; Jiang, Longguang;

    2013-01-01

    The development of protease inhibitors for pharmacological intervention has taken a new turn with the use of peptide-based inhibitors. Here, we report the rational design of bicyclic peptide inhibitors of the serine protease urokinase-type plasminogen activator (uPA), based on the established...

  11. The pro-urokinase plasminogen-activation system in the presence of serpin-type inhibitors and the urokinase receptor

    DEFF Research Database (Denmark)

    Behrendt, Niels; List, Karin; Andreasen, Peter A;

    2003-01-01

    The reciprocal pro-enzyme activation system of plasmin, urokinase-type plasminogen activator (uPA) and their respective zymogens is a potent mechanism in the generation of extracellular proteolytic activity. Plasminogen activator inhibitor type 1 (PAI-1) acts as a negative regulator. This system ...

  12. Characterization of urokinase receptor expression by human placental trophoblasts.

    Science.gov (United States)

    Zini, J M; Murray, S C; Graham, C H; Lala, P K; Karikó, K; Barnathan, E S; Mazar, A; Henkin, J; Cines, D B; McCrae, K R

    1992-06-01

    The processes of implantation and placentation are both dependent on the invasion and remodeling of the uterine endometrium and vasculature by trophoblasts. Because the secretion and autocrine binding of urokinase (uPA) appears to be a common mechanism used by cells to facilitate plasmin-dependent tissue invasion, we measured the production of uPA and expression of uPA receptors by trophoblasts. Prourokinase bound specifically, reversibly, and with high affinity to cultured trophoblasts, via the uPA epidermal growth factor-like domain. Trophoblasts derived from two first-trimester placentae bound more prourokinase than cells isolated from term placentae. Furthermore, in vitro differentiation of cultured cytotrophoblasts into syncytiotrophoblasts was associated with diminished expression of urokinase receptors and a parallel decrease in the cellular content of uPA receptor mRNA. Trophoblasts also secreted prourokinase and plasminogen activator inhibitors types 1 and 2 (PAI-1 and PAI-2). Although prourokinase was secreted in amounts sufficient to endogenously saturate trophoblast uPA receptors, trophoblasts secreted greater amounts of PAI-1 and PAI-2 than uPA, and no net plasminogen activator activity was detected in trophoblast conditioned medium. In contrast, plasminogen added directly to cultured trophoblasts was readily converted to plasmin. Although the invasion and remodeling of uterine tissues by trophoblasts is a complex process dependent on several proteases of varying specificity, our findings suggest that the expression and modulation of urokinase receptors on the trophoblast cell surface may play an important role in this process. PMID:1316787

  13. Plasma soluble urokinase plasminogen activator receptor in children with urinary tract infection

    DEFF Research Database (Denmark)

    Wittenhagen, Per; Andersen, Jesper Brandt; Hansen, Anita;

    2011-01-01

    In this prospective study we investigated the role of plasma levels of soluble urokinase plasminogen activator receptor (suPAR) in children with urinary tract infection.......In this prospective study we investigated the role of plasma levels of soluble urokinase plasminogen activator receptor (suPAR) in children with urinary tract infection....

  14. Urokinase receptor forms in serum from non-small cell lung cancer patients

    DEFF Research Database (Denmark)

    Almasi, Charlotte Elberling; Christensen, Ib Jarle; Høyer-Hansen, Gunilla;

    2011-01-01

    To study the prognostic impact of the different forms of the receptor for urokinase plasminogen activator (uPAR) in serum from 171 non-small cell lung cancer (NSCLC) patients.......To study the prognostic impact of the different forms of the receptor for urokinase plasminogen activator (uPAR) in serum from 171 non-small cell lung cancer (NSCLC) patients....

  15. Purification of urokinase by combined cation exchanger and affinity chromatographic cartridges.

    Science.gov (United States)

    Hou, K C; Zaniewski, R

    1990-02-23

    Crude urokinase from human urine processed through foam flotation and ammonium sulfate precipitation containing 720 National Health Institute Committee on Thrombolytic Agents U/mg activity was purified by an SP cation exchanger followed by a zinc-chelated affinity chromatographic cartridge. The cartridges were of a radial-flow type formed by using acrylic and cellulose composite matrices. The high rigidity of the matrix structure permits fast flow of protein solutions (liters per minute) and thus allows processing of a large volume of crude urokinase under low operating pressures. A greater than six-fold increase in specific enzyme activity of urokinase was achieved by adsorbing and eluting 1 l of a 3 mg/ml crude urokinase solution on an SP cartridge. The eluent was further purified by passing through a zinc-chelated affinity cartridge to achieve greater than a eighteen-fold increase in urokinase specific activity. This report demonstrates the combined use of a cation exchanger with zinc-chelated chromatographic cartridges in purifying urokinase on a relatively large scale. The relationship between the amount of zinc chelated in the matrix to its effect on urokinase purification is also discussed. PMID:2329161

  16. Transgenic chickens expressing human urokinase-type plasminogen activator.

    Science.gov (United States)

    Lee, Sung Ho; Gupta, Mukesh Kumar; Ho, Young Tae; Kim, Teoan; Lee, Hoon Taek

    2013-09-01

    Urokinase-type plasminogen activator is a serine protease that is clinically used in humans for the treatment of thrombolytic disorders and vascular diseases such as acute ischemic stroke and acute peripheral arterial occlusion. This study explored the feasibility of using chickens as a bioreactor for producing human urokinase-type plasminogen activator (huPA). Recombinant huPA gene, under the control of a ubiquitous Rous sarcoma virus promoter, was injected into the subgerminal cavity of freshly laid chicken eggs at stage X using the replication-defective Moloney murine leukemia virus (MoMLV)-based retrovirus vectors encapsidated with VSV-G (vesicular stomatitis virus G) glycoprotein. A total of 38 chicks, out of 573 virus-injected eggs, hatched and contained the huPA gene in their various body parts. The mRNA transcript of the huPA gene was present in various organs, including blood and egg, and was germ-line transmitted to the next generation. The level of active huPA protein was 16-fold higher in the blood of the transgenic chicken than in the nontransgenic chicken (P pharming of the huPA drug but also be useful for studying huPA-induced bleeding and other disorders. PMID:23960123

  17. Transgenic chickens expressing human urokinase-type plasminogen activator.

    Science.gov (United States)

    Lee, Sung Ho; Gupta, Mukesh Kumar; Ho, Young Tae; Kim, Teoan; Lee, Hoon Taek

    2013-09-01

    Urokinase-type plasminogen activator is a serine protease that is clinically used in humans for the treatment of thrombolytic disorders and vascular diseases such as acute ischemic stroke and acute peripheral arterial occlusion. This study explored the feasibility of using chickens as a bioreactor for producing human urokinase-type plasminogen activator (huPA). Recombinant huPA gene, under the control of a ubiquitous Rous sarcoma virus promoter, was injected into the subgerminal cavity of freshly laid chicken eggs at stage X using the replication-defective Moloney murine leukemia virus (MoMLV)-based retrovirus vectors encapsidated with VSV-G (vesicular stomatitis virus G) glycoprotein. A total of 38 chicks, out of 573 virus-injected eggs, hatched and contained the huPA gene in their various body parts. The mRNA transcript of the huPA gene was present in various organs, including blood and egg, and was germ-line transmitted to the next generation. The level of active huPA protein was 16-fold higher in the blood of the transgenic chicken than in the nontransgenic chicken (P pharming of the huPA drug but also be useful for studying huPA-induced bleeding and other disorders.

  18. Clinical study of urokinase-bound fibrocollagenous tubes.

    Science.gov (United States)

    Senatore, F; Bernath, F; Meisner, K

    1986-02-01

    Dacron-reinforced fibrocollagenous tubes (FCT) were synthesized from canine mongrels using the mandril-rod technique in order to develop a small diameter (i.e., 4 mm i.d.) vascular graft. They were rendered fibrinolytic by immobilizing urokinase on to the inner surface of the tubes. Urokinase-bound fibrocollagenous tubes (UK-FCT), control FCTs (i.e., no bound enzyme), Perloff grafts (Dr. Perloff, Department of Surgery, University Medical Center, Sidney Australia, has developed a mandril-derived collagenous tube from goats. Samples were implanted for comparative purposes.) and autogeneous saphenous veins, were interposed in the carotid or femoral artery in chronic studies involving 21 canine mongrels. On the basis of Doppler auscultation and palpation, the UK-FCTs were statistically more patent than other candidate prostheses. Fibrin degradation product (FDP) increased in the dogs' systemic circulation with a postoperative peak of 5 days. The host's increase in fibrinolytic activity was shown to be local to the anastamosis. A carotid arterial extracorporeal shunt was designed to evaluate acute patency. Results indicated a rapid thrombosis but no platlet or fibrin adherence to the graft surface was observed, as evidenced by scanning electron microscopy. PMID:2937788

  19. A Novel Gene Delivery System Targeting Urokinase Receptor

    Institute of Scientific and Technical Information of China (English)

    Xing-Hui SUN; Li TAN; Chun-Yang LI; Chang TONG; Jin FAN; Ping LI; Yun-Song ZHU

    2004-01-01

    Recombinant proteins that combine different functions required for cell targeting and intracellular delivery of DNA present an attractive approach for the development of nonviral gene delivery vectors. Here, we described a novel protein termed ATF-lys10 which facilitated cell-specific gene transfer via receptor-mediated endocytosis. ATF-lys 10 was composed of the amino-terminal fragment of urokinase and ten lysines at the carboxyl terminus. Bacterially expressed ATF-lys 10 protein existed in soluble form, and had antigenicity of human urokinase. Purified ATF-lys 10 specifically bound to uPAR-expressing cells and formed protein-DNA complexes with plasmid pGL3-control. After neutralization of excess negative charge with poly-L-lysine, these complexes served as a specific gene delivery vector for uPAR-expressing cells. Lysosomotropic compounds, such as chloroquine, drastically increased the ATF-lysl0 mediated gene delivery efficiency. Our results suggest that the recombinant protein ATF-lys 10 with the properties of DNA binding and tumor cell targeting represents a promising method for gene transfer and expression in tumor cells.

  20. Structural investigations of recombinant urokinase growth factor-like domain.

    Science.gov (United States)

    Beloglazova, I B; Beabealashvilli, R Sh; Gursky, Ya G; Bocharov, E V; Mineev, K S; Parfenova, E V; Tkachuk, V A

    2013-05-01

    Urokinase-type plasminogen activator (uPA) is a serine protease that converts the plasminogen zymogen into the enzymatically active plasmin. uPA is synthesized and secreted as the single-chain molecule (scuPA) composed of an N-terminal domain (GFD) and kringle (KD) and C-terminal proteolytic (PD) domains. Earlier, the structure of ATF (which consists of GFD and KD) was solved by NMR (A. P. Hansen et al. (1994) Biochemistry, 33, 4847-4864) and by X-ray crystallography alone and in a complex with the soluble form of the urokinase receptor (uPAR, CD87) lacking GPI (C. Barinka et al. (2006) J. Mol. Biol., 363, 482-495). According to these data, GFD contains two β-sheet regions oriented perpendicularly to each other. The area in the GFD responsible for binding to uPAR is localized in the flexible Ω-loop, which consists of seven amino acid residues connecting two strings of antiparallel β-sheet. It was shown by site-directed mutagenesis that shortening of the Ω-loop length by one amino acid residue leads to the inability of GFD to bind to uPAR (V. Magdolen et al. (1996) Eur. J. Biochem., 237, 743-751). Here we show that, in contrast to the above-mentioned studies, we found no sign of the β-sheet regions in GFD in our uPA preparations either free or in a complex with uPAR. The GFD seems to be a rather flexible and unstructured domain, demonstrating in spite of its apparent flexibility highly specific interaction with uPAR both in vitro and in cell culture experiments. Circular dichroism, tryptophan fluorescence during thermal denaturation of the protein, and heteronuclear NMR spectroscopy of ¹⁵N/¹³C-labeled ATF both free and in complex with urokinase receptor were used to judge the secondary structure of GFD of uPA. PMID:23848154

  1. Enhanced levels of urokinase plasminogen activator and its soluble receptor in common variable immunodeficiency

    DEFF Research Database (Denmark)

    Fevang, Børre; Eugen-Olsen, Jesper; Yndestad, Arne;

    2009-01-01

    Common variable immunodeficiency (CVID) is a heterogeneous syndrome characterized by defective immunoglobulin production and high frequency of bacterial infections, autoimmunity and manifestations of chronic inflammation. The urokinase plasminogen activator (uPA), its cell bound and soluble recep...

  2. Expression of urokinase receptors by human trophoblast. A histochemical and ultrastructural analysis

    DEFF Research Database (Denmark)

    Multhaupt, H A; Mazar, A; Cines, D B;

    1994-01-01

    BACKGROUND: Through their ability to invade endometrium, remodel the uterine spiral arteries, and sustain placental blood fluidity, trophoblast cells play a central role in establishing and maintaining the integrity of the uteroplacental vasculature. The expression of urokinase receptors by troph...

  3. Continuous quality improvement: improving hemodialysis catheter patency using urokinase.

    Science.gov (United States)

    Northsea, C

    1996-12-01

    Opportunities for improvements in patient outcomes through applied continuous quality improvement (CQI) programs are endless and exciting. Improving vascular access outcomes has been a long-standing clinical problem for hemodialysis patients and the nephrology team. During the past few years there has been a dramatic increase in the use of dialysis catheters as permanent accesses for hemodialysis patients. All hemodialysis with dialysis catheters are at risk for catheter occlusion. An innovative, 2-year CQI program was developed, implemented, and designed to improve dialysis catheter patency rates with the use of urokinase. The CQI program resulted in a number of clinical outcomes that were beneficial to the patients and dialysis staff, and were cost-effective to the program.

  4. Macroeconomic regimes

    NARCIS (Netherlands)

    Baele, L.T.M.; Bekaert, G.R.J.; Cho, S.; Inghelbrecht, K.; Moreno, A.

    2015-01-01

    A New-Keynesian macro-model is estimated accommodating regime-switching behavior in monetary policy and macro-shocks. A key to our estimation strategy is the use of survey-based expectations for inflation and output. Output and inflation shocks shift to the low volatility regime around 1985 and 1990

  5. The urokinase plasminogen activator receptor-associated protein/endo180 is coexpressed with its interaction partners urokinase plasminogen activator receptor and matrix metalloprotease-13 during osteogenesis

    DEFF Research Database (Denmark)

    Engelholm, L H; Nielsen, B S; Netzel-Arnett, S;

    2001-01-01

    The urokinase plasminogen activator receptor-associated protein/Endo180 (uPARAP/Endo180) is a newly discovered member of the macrophage mannose receptor family that was reported to interact with ligand-bound urokinase plasminogen activator receptor (uPAR), matrix metalloprotease-13 (MMP-13), and ......, uPARAP/Endo180 expression was detected only in a mesenchymal condensation of the midbrain and in the developing lungs. The data suggest a function of this novel protease receptor in bone development, possibly mediated through its interactions with uPAR, MMP-13, or collagen V....

  6. Prolonged radiation damage in rat colon and urokinase expression in epithelium

    Energy Technology Data Exchange (ETDEWEB)

    Hayashida, Masayuki; Minami, Kazunori; Okimoto, Tomoaki [Nagasaki Univ. (Japan). School of Medicine; Shichijo, Kazuko; Matsuu, Mutsumi; Nakayama, Toshiyuki; Sekine, Ichiro

    2001-12-01

    Although radiation therapy plays important role in the treatment of gynecological tumors, it may cause radiation injury as a late effect. Several recent reports show that urokinase such as urokinase type plasminogen activator (uPA) contributes to the repair of ulcerative lesions of the colon epithelium. We studied radiation induced enterocolitis using rat animal models. Seventy-two female Wistar rats were irradiated by a single fraction dose of 36 Gy at laparotomy. Histological changes and activity of urokinase system were investigated after irradiation. Ulcers were observed in irradiated field in 12 of 19 animals (63%) even at 60th week after irradiation. Urokinase expressions were observed in the margins of active ulcer. Urokinase was thought to play important role in exacerbation of ulcer formation. Expression of uPA was also observed in submucosal glands. Ischaemic changes were not observed in irradiated colon despite sclerosing vasculitis. It is suggested that uPA played reciprocal roles in radiation induced enterocolitis: healing and aggravation of ulcer. (author)

  7. Cell-surface acceleration of urokinase-catalyzed receptor cleavage

    DEFF Research Database (Denmark)

    Høyer-Hansen, G; Ploug, M; Behrendt, N;

    1997-01-01

    The urokinase-type plasminogen activator (uPA) binds to a specific cell-surface receptor, uPAR. On several cell types uPAR is present both in the full-length form and a cleaved form, uPAR(2+3), which is devoid of binding activity. The formation of uPAR(2+3) on cultured U937 cells is either directly...... by a prior incubation of the cells with uPA inactivated by diisopropyl fluorophosphate, demonstrating a requirement for specific receptor binding of the active uPA to obtain the high-efficiency cleavage of cell-bound uPAR. Furthermore, amino-terminal sequence analysis revealed that uPAR(2+3), purified from U......937 cell lysates, had the same amino termini as uPAR(2+3), generated by uPA in a purified system. In both cases cleavage had occurred at two positions in the hinge region connecting domain 1 and 2, between Arg83-Ala84 and Arg89-Ser90, respectively. The uPA-catalyzed cleavage of uPAR is a new negative...

  8. Application of Molecular Modeling to Urokinase Inhibitors Development

    Directory of Open Access Journals (Sweden)

    V. B. Sulimov

    2014-01-01

    Full Text Available Urokinase-type plasminogen activator (uPA plays an important role in the regulation of diverse physiologic and pathologic processes. Experimental research has shown that elevated uPA expression is associated with cancer progression, metastasis, and shortened survival in patients, whereas suppression of proteolytic activity of uPA leads to evident decrease of metastasis. Therefore, uPA has been considered as a promising molecular target for development of anticancer drugs. The present study sets out to develop the new selective uPA inhibitors using computer-aided structural based drug design methods. Investigation involves the following stages: computer modeling of the protein active site, development and validation of computer molecular modeling methods: docking (SOL program, postprocessing (DISCORE program, direct generalized docking (FLM program, and the application of the quantum chemical calculations (MOPAC package, search of uPA inhibitors among molecules from databases of ready-made compounds to find new uPA inhibitors, and design of new chemical structures and their optimization and experimental examination. On the basis of known uPA inhibitors and modeling results, 18 new compounds have been designed, calculated using programs mentioned above, synthesized, and tested in vitro. Eight of them display inhibitory activity and two of them display activity about 10 μM.

  9. Regime change?

    International Nuclear Information System (INIS)

    Following the 1998 nuclear tests in South Asia and later reinforced by revelations about North Korean and Iraqi nuclear activities, there has been growing concern about increasing proliferation dangers. At the same time, the prospects of radiological/nuclear terrorism are seen to be rising - since 9/11, concern over a proliferation/terrorism nexus has never been higher. In the face of this growing danger, there are urgent calls for stronger measures to strengthen the current international nuclear nonproliferation regime, including recommendations to place civilian processing of weapon-useable material under multinational control. As well, there are calls for entirely new tools, including military options. As proliferation and terrorism concerns grow, the regime is under pressure and there is a temptation to consider fundamental changes to the regime. In this context, this paper will address the following: Do we need to change the regime centered on the Treaty on the Nonproliferation of Nuclear Weapons (NPT) and the International Atomic Energy Agency (IAEA)? What improvements could ensure it will be the foundation for the proliferation resistance and physical protection needed if nuclear power grows? What will make it a viable centerpiece of future nonproliferation and counterterrorism approaches?

  10. Adsorption and Step Elution of Urokinase Using, Affinity Chromatography -Comparison of Data with Rate Model Simulation

    Institute of Scientific and Technical Information of China (English)

    MohammadRezaAboudzadehRovais; JiawenZhu; BinWu

    2004-01-01

    A non-equilibrium chromatographic rate model was employed to simulate the affinity chromatography of urokinase. The chromatography process was developed to a yield of high purity product of urokinase from crude materials. The affinity gel used in the process was prepared by an epichlorohydrin-activation method using epichlorohydrin activated Sepharose 4B as a matrix and p-aminobenzamidine as a ligand. The chromatographic process were numerically simulated and analyzed with the aid of VERSE-LC computer simulator. Considering the basic principles, rate model with the back mixing in column inlet was utilized in simulating and studying the effect of the column inlet pattern on other parameters. Comparison of the simulation results with the experimental data showed that the rate model can be used to describe the affinity chromatography of urokinase in a fixed bed column with satisfactory accuracy.

  11. Two distinct expression patterns of urokinase, urokinase receptor and plasminogen activator inhibitor-1 in colon cancer liver metastases

    DEFF Research Database (Denmark)

    Illemann, Martin; Bird, Nigel; Majeed, Ali;

    2009-01-01

    Metastatic growth and invasion by colon cancer cells in the liver requires the ability of the cancer cells to interact with the new tissue environment. Plasmin(ogen) is activated on cell surfaces by urokinase-type PA (uPA), and is regulated by uPAR and plasminogen activator inhibitor-1 (PAI-1......). To compare the expression patterns of uPA, uPAR and PAI-1 in colon cancer with that in their liver metastases, we analysed matched samples from 14 patients. In all 14 primary colon cancers, we found upregulation of uPAR, uPA mRNA and PAI-1 in primarily stromal cells at the invasive front. In 5 of the 14......, whereas 8 of the remaining 9 showed direct contact between the cancer cells and the liver parenchyma. We conclude that there are 2 distinct patterns of expression of uPAR, uPA and PAI-1 in colon cancer liver metastases and that these correlate closely with 2 morphological growth patterns. These findings...

  12. Efficacy of 2-hour post glucose insulin levels in predicting insulin resistance in polycystic ovarian syndrome with infertility

    Science.gov (United States)

    Saxena, Pikee; Prakash, Anupam; Nigam, Aruna

    2011-01-01

    BACKGROUND: Insulin resistance (IR) is central to the pathogenesis of polycystic ovarian syndrome (PCOS), but tests for determining IR are elaborate, tedious and expensive. AIMS: To evaluate if “2-hour post-glucose insulin level” is an effective indicator of IR and can aid in diagnosing IR in infertile PCOS women. SETTINGS AND DESIGN: Observational study at infertility clinic of a tertiary care center. MATERIALS AND METHODS: 50 infertile women with PCOS and 20 females with tubal/male factor infertility were evaluated for the presence of IR, as defined by the fasting/2-hour post-glucose insulin levels cutoffs of >25/>41 μU/mL, respectively. The clinical, metabolic and endocrinologic profile was determined in both the groups. STATISTICAL ANALYSIS: Statistical analysis was performed using SPSS (Chicago, IL, USA). RESULTS: Body mass index, post load glucose, insulin, glucose/insulin ratio, area under curve (AUC) of glucose and insulin and insulinogenic index were significantly lower in the controls as compared to the PCOS group. “2-hour post-glucose insulin levels” were elevated in 88% of PCOS individuals but were normal in all females not suffering from PCOS. These levels significantly correlated with AUC of glucose and insulin, and insulinogenic index and inversely correlated with 2-hour glucose to insulin ratio (r=0.827, 0.749 and –0.732, respectively). CONCLUSIONS: “2-hour post-glucose insulin levels” appears to be a good indicator of IR. It can be a useful tool, especially in low resource setting where a single sample can confirm the diagnosis, thus reducing cost and repeat visits. PMID:21772735

  13. Efficacy of 2-hour post glucose insulin levels in predicting insulin resistance in polycystic ovarian syndrome with infertility

    Directory of Open Access Journals (Sweden)

    Pikee Saxena

    2011-01-01

    Full Text Available Background : Insulin resistance (IR is central to the pathogenesis of polycystic ovarian syndrome (PCOS, but tests for determining IR are elaborate, tedious and expensive. Aims : To evaluate if "2-hour post-glucose insulin level" is an effective indicator of IR and can aid in diagnosing IR in infertile PCOS women. Settings and Design : Observational study at infertility clinic of a tertiary care center. Materials and Methods : 50 infertile women with PCOS and 20 females with tubal/male factor infertility were evaluated for the presence of IR, as defined by the fasting/2-hour post-glucose insulin levels cutoffs of >25/>41 μU/mL, respectively. The clinical, metabolic and endocrinologic profile was determined in both the groups. Statistical Analysis : Statistical analysis was performed using SPSS (Chicago, IL, USA. Results : Body mass index, post load glucose, insulin, glucose/insulin ratio, area under curve (AUC of glucose and insulin and insulinogenic index were significantly lower in the controls as compared to the PCOS group. "2-hour post-glucose insulin levels" were elevated in 88% of PCOS individuals but were normal in all females not suffering from PCOS. These levels significantly correlated with AUC of glucose and insulin, and insulinogenic index and inversely correlated with 2-hour glucose to insulin ratio (r=0.827, 0.749 and −0.732, respectively. Conclusions : "2-hour post-glucose insulin levels" appears to be a good indicator of IR. It can be a useful tool, especially in low resource setting where a single sample can confirm the diagnosis, thus reducing cost and repeat visits.

  14. Neuroprotection by urokinase plasminogen activator in the hippocampus.

    Science.gov (United States)

    Cho, Eunsil; Lee, Kyung Jin; Seo, Jung-Woo; Byun, Catherine Jeonghae; Chung, Sun-Ju; Suh, Dae Chul; Carmeliet, Peter; Koh, Jae-Young; Kim, Jong S; Lee, Joo-Yong

    2012-04-01

    Tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), which are both used for thrombolytic treatment of acute ischemic stroke, are serine proteases that convert plasminogen to active plasmin. Although recent experimental evidences have raised controversy about the neurotoxic versus neuroprotective roles of tPA in acute brain injury, uPA remains unexplored in this context. In this study, we evaluated the effect of uPA on neuronal death in the hippocampus of mice after kainate-induced seizures. In the normal brain, uPA was localized to both nuclei and cytosol of neurons. Following severe kainate-induced seizures, uPA completely disappeared in degenerating neurons, whereas uPA-expressing astrocytes substantially increased, suggesting reactive astrogliosis. uPA-knockout mice were more vulnerable to kainate-induced neuronal death than wild-type mice. Consistent with this, inhibition of uPA by intracerebral injection of the uPA inhibitor UK122 increased the level of neuronal death. In contrast, prior administration of recombinant uPA significantly attenuated neuronal death. Collectively, these results indicate that uPA renders neurons resistant to kainate-induced excitotoxicity. Moreover, recombinant uPA suppressed cell death in primary cultures of hippocampal neurons exposed to H2O2, zinc, or various excitotoxins, suggesting that uPA protects against neuronal injuries mediated by the glutamate receptor, or by oxidation- or zinc-induced death signaling pathways. Considering that tPA may facilitate neurodegeneration in acute brain injury, we suggest that uPA, as a neuroprotectant, might be beneficial for the treatment of acute brain injuries such as ischemic stroke.

  15. Competition Regime

    OpenAIRE

    Danilo Icaza Ortiz

    2013-01-01

    This paper is a review of the competition regime works of various authors, published under the auspices of the University of the Hemispheres and the Corporation for Studies and Publications. Analyzes the structure, the general concepts, case law taken for development. Includes comments on the usefulness of this work for the study of competition law and the contribution to the lawyers who want to practice in this branch of economic law.

  16. Competition Regime

    Directory of Open Access Journals (Sweden)

    Danilo Icaza Ortiz

    2013-01-01

    Full Text Available This paper is a review of the competition regime works of various authors, published under the auspices of the University of the Hemispheres and the Corporation for Studies and Publications. Analyzes the structure, the general concepts, case law taken for development. Includes comments on the usefulness of this work for the study of competition law and the contribution to the lawyers who want to practice in this branch of economic law.

  17. Activation of human pro-urokinase by unrelated proteases secreted by Pseudomonas aeruginosa : LasB and protease IV activate human pro-urokinase

    OpenAIRE

    Beaufort, Nathalie; Seweryn, Paulina; De Bentzmann, Sophie; Tang, Aihua; Kellermann, Josef; Grebenchtchikov, Nicolai; Schmitt, Manfred; Sommerhoff, Christian,; Pidard, Dominique; Magdolen, Viktor

    2010-01-01

    Abstract Pathogenic bacteria, including Pseudomonas aeruginosa, interact with and engage the host plasminogen (Plg) activation system, which encompasses the urokinase(uPA)-type Plg activator, and is involved in extracellular proteolysis, including matrilysis and fibrinolysis. We hypothesized that secreted bacterial proteases might contribute to the activation of this major extracellular proteolytic system, thereby participating in bacterial dissemination. We report that LasB, a...

  18. Effects of acute and 2-hour postphysical activity on the estimation of body fat made by the bod pod.

    Science.gov (United States)

    Harrop, Bradley J; Woodruff, Sarah J

    2015-06-01

    The Bod Pod has been found to be reliable/valid against several criterion methods, including hydrostatic weighing and dual-energy x-ray absorptiometry, and under different conditions, such as clothing, dehydrated states, and body temperature changes. However, questions remain regarding the effects of an acute bout of exercise. Therefore, the purpose was to determine the effects of an acute bout of exercise on the estimations made by the Bod Pod. Participants (15 men and 22 women) were of age 18-27 years and were currently exercising. Baseline Bod Pod measures were completed followed by a 30-minute cycling trial at 75% of maximum heart rate. Bod Pod measures were taken immediately after exercise and 2 hours after exercise. Differences between men and women were found at baseline between height (p Bod Pod testing seems to alter the estimate of %BF, and continues to affect the prediction 2 hours after exercise in women.

  19. Efficacy of 2-hour post glucose insulin levels in predicting insulin resistance in polycystic ovarian syndrome with infertility

    OpenAIRE

    Pikee Saxena; Anupam Prakash; Aruna Nigam

    2011-01-01

    Background : Insulin resistance (IR) is central to the pathogenesis of polycystic ovarian syndrome (PCOS), but tests for determining IR are elaborate, tedious and expensive. Aims : To evaluate if "2-hour post-glucose insulin level" is an effective indicator of IR and can aid in diagnosing IR in infertile PCOS women. Settings and Design : Observational study at infertility clinic of a tertiary care center. Materials and Methods : 50 infertile women with PCOS and 20 females with tubal/male fact...

  20. The urokinase receptor (uPAR) facilitates clearance of Borrelia burgdorferi

    NARCIS (Netherlands)

    J.W.R. Hovius; M.F. Bijlsma; G.J.W. van der Windt; W.J. Wiersinga; B.J.D. Boukens; J. Coumou; A. Oei; R. de Beer; A.F. de Vos; C. van 't Veer; A.P. van Dam; P. Wang; E. Fikrig; M.M. Levi; J.J.T.H. Roelofs; T. van der Poll

    2009-01-01

    The causative agent of Lyme borreliosis, the spirochete Borrelia burgdorferi, has been shown to induce expression of the urokinase receptor (uPAR); however, the role of uPAR in the immune response against Borrelia has never been investigated. uPAR not only acts as a proteinase receptor, but can also

  1. Jet and ultrasonic nebulization of single chain urokinase plasminogen activator (scu-PA)

    DEFF Research Database (Denmark)

    Münster, Anna-Marie; Bendstrup, E; Jensen, J.I.;

    2000-01-01

    Recent studies have indicated that the deposition of intra-alveolar fibrin may play a central role in the pathogenesis of acute respiratory distress syndrome (ARDS). Our aim was to study whether the indigenous fibrinolytic agent (urokinase) normally present in the alveoli can be administered...

  2. Superselective Urokinase Infusion Therapy for Dorsalis Pedis Artery Occlusion in Buerger's Disease

    International Nuclear Information System (INIS)

    Occlusion of the proximal left dorsalis pedis artery (DPA) in a patient with Buerger's disease was treated by continuous urokinase intraarterial infusion using a microcatheter. Recanalization of the DPA and healing of a toe ulcer were achieved. The patient remains asymptomatic during a 4-year follow-up

  3. Tissue plasminogen activator and urokinase plasminogen activator in human epileptogenic pathologies

    NARCIS (Netherlands)

    A.M. Iyer; E. Zurolo; K. Boer; J.C. Baayen; F. Giangaspero; A. Arcella; G.C. Di Gennaro; V. Esposito; W.G.M. Spliet; P.C. van Rijen; D. Troost; J.A. Gorter; E. Aronica

    2010-01-01

    A growing body of evidence demonstrates the involvement of plasminogen activators (PAs) in a number of physiologic and pathologic events in the CNS. Induction of both tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) has been observed in different experimental models of ep

  4. Soluble Urokinase Plasminogen Activator Receptor for Risk Prediction in Patients Admitted with Acute Chest Pain

    DEFF Research Database (Denmark)

    Lyngbæk, Stig; Andersson, Charlotte; Marott, Jacob L;

    2013-01-01

    Plasma concentrations of soluble urokinase plasminogen activator receptor (suPAR) predict mortality in several clinical settings, but the long-term prognostic importance of suPAR in chest pain patients admitted on suspicion of non-ST-segment elevation acute coronary syndrome (NSTEACS) is uncertain....

  5. Circulating intact and cleaved forms of the urokinase-type plasminogen activator receptor

    DEFF Research Database (Denmark)

    Røndbjerg, Tine Thurison; Christensen, Ib J; Lund, Ida K;

    2015-01-01

    BACKGROUND: High levels of circulating forms of the urokinase-type plasminogen activator receptor (uPAR) are significantly associated to poor prognosis in cancer patients. Our aim was to determine biological variations and reference intervals of the uPAR forms in blood, and in addition, to test t...

  6. Characterization of human endothelial cell urokinase-type plasminogen activator receptor protein and messenger RNA

    DEFF Research Database (Denmark)

    Barnathan, E S; Kuo, A; Karikó, K;

    1990-01-01

    Human umbilical vein endothelial cells in culture (HUVEC) express receptors for urokinase-type plasminogen activators (u-PA). The immunochemical nature of this receptor and its relationship to u-PA receptors expressed by other cell types is unknown. Cross-linking active site-blocked u-PA to HUVEC...

  7. Elevated plasma urokinase receptor predicts low birth weight in maternal malaria

    DEFF Research Database (Denmark)

    Ostrowski, S R; Shulman, C E; Peshu, N;

    2007-01-01

    The blood level of soluble urokinase receptor (suPAR) is increased and associated with a poor clinical or fatal outcome in children with acute malaria. This study hypothesized that the suPAR level would be associated with foetal outcome in maternal malaria. suPAR was measured by ELISA in maternal...

  8. Urokinase-type plasminogen activator receptor as a predictor of poor outcome in patients with systemic inflammatory response syndrome

    OpenAIRE

    Wu, Xiao-Ling; Long, Ding; Yu, Li; Yang, Jun-hui; Zhang, Yuan-chao; Geng, Feng

    2013-01-01

    BACKGROUND: Urokinase-type plasminogen activator (uPA) and urokinase-type plasminogen activator receptor (uPAR) are known as important factors, which mediate a variety of functions in terms of vascular homeostasis, inflammation and tissue repair. However, their role in systemic inflammatory response syndrome (SIRS) has been less well studied. This study aimed to test the hypothesis that the abnormalities of fibrinolysis and degradation of extracellular matrix mediated by uPA and uPAR are dire...

  9. Effect of urokinase on the proliferation of primary cultures of human prostatic cells

    International Nuclear Information System (INIS)

    The effects of exogenously added urokinase type plasminogen activator, tissue type plasminogen activator, plasmin and thrombin on the proliferation of primary cultures of cells derived from prostatic hyperplasia or prostatic carcinomas were investigated by measuring the incorporation of 3H-thymidine into the cultures. Addition of urokinase type plasminogen activator (1.35 x 10(-9) M) or thrombin (10(-7) M) to the culture medium caused a two-fold increase of 3H-thymidine incorporation, regardless of the origin of the prostatic cells. Tissue type plasminogen activator did not alter the rate of 3H-thymidine incorporation, whereas plasmin caused a 25% decrease of 3H-thymidine incorporation in all cultures

  10. Dictyostelium discoideum: a model for testing novel inhibitors of urokinase-type plasminogen activator

    OpenAIRE

    Thompson, Elinor

    2013-01-01

    The social amoeba Dictyostelium discoideum is a useful non-animal eukaryote for testing novel compounds and dissecting cell regulatory molecular networks. We used this model organism to investigate the effect of a series of arylboronic acids and pinacol esters on development, chemotaxis and viability. These compounds were studied in parallel by collaborators for serine protease and urokinase-type plasminogen activator (uPA) inhibition, both in vitro and in vivo. In those biochemical assays, t...

  11. Urokinase receptor cleavage correlates with tumor volume in a transgenic mouse model of breast cancer

    DEFF Research Database (Denmark)

    Thurison, Tine; Almholt, Kasper; Gårdsvoll, Henrik;

    2015-01-01

    marker for cancer than increased expression of muPAR per se. The levels of the muPAR forms are dramatically affected by in vivo challenge with a urokinase -blocking antibody, demonstrating a functional role of uPA in uPAR cleavage. The levels of the muPAR forms are, however, unaffected by u......The urokinase plasminogen activator system plays a key role in tissue degradation during cancer invasion. The linker region between domains I and II of the intact, three domain urokinase receptor uPAR(I-III) is highly susceptible to proteolytic cleavage and the resulting cleaved uPAR forms...... are strong prognostic biomarkers in several types of cancer, i.e., high levels of the cleaved uPAR forms indicate poor survival. To better understand the role of uPAR cleavage in cancer, we have designed immunoassays for specific quantification of intact mouse uPAR [muPAR(I-III)] and mouse uPAR domain I [mu...

  12. Urokinase perfusion prevents intrahepatic ischemic-type biliary lesion in donor livers

    Institute of Scientific and Technical Information of China (English)

    Ren Lang; Qiang He; Zhong-Kui Jin; Dong-Dong Han; Da-Zhi Chen

    2009-01-01

    AIM: To evaluate whether urokinase perfusion of non-heart-beating cadaveric donor livers reduces the incidence of intrahepatic ischemic-type biliary lesions (IITBLs). METHODS: A prospective study was conducted to investigate potential microthrombosis in biliary microcirculation when non-heart-beating cadaveric livers were under warm or cold ischemic conditions. The experimental group included 140 patients who underwent liver transplantation during the period of January 2006 to December 2007, and survived for more than 1 year. The control group included 220 patients who received liver transplantation between July 1999 and December 2005 and survived for more than 1 year. In the experimental group, the arterial system of the donor liver was perfused twice with urokinase during cold perfusion and after trimming of the donor liver. The incidence of IITBLs was compared between the two groups. RESULTS: In the control group, the incidence of IITBLs was 5.9% (13/220 cases) after 3-11 mo of transplantation. In the experimental group, two recipients (1.4%) developed IITBLs at 3 and 6 mo after transplantation, respectively. The difference in the incidence between the two groups was statistically significant ( P < 0.05). CONCLUSION: Double perfusion of cadaveric livers from non-heart-beating donors with urokinase may reduce the incidence of IITBLs.

  13. Correlation of 2 hour, 4 hour, 8 hour and 12 hour urine protein with 24 hour urinary protein in preeclampsia.

    Directory of Open Access Journals (Sweden)

    Savita Rani Singhal

    2014-09-01

    Full Text Available To find shortest and reliable time period of urine collection for determination of proteinuria.It is a prospective study carried out on 125 pregnant women with preeclampsia after 20 weeks of gestation having urine albumin >1 using dipstick test. Urine was collected in five different time intervals in colors labeled containers with the assistance of nursing staff; the total collection time was 24 hours. Total urine protein of two-hour, four-hour, eight-hour, 12-hour and 24-hour urine was measured and compared with 24-hour collection. Data was analyzed using the Pearson correlation coefficient.There was significant correlation (p value < 0.01 in two, four, eight and 12-hour urine protein with 24-urine protein, with correlation coefficient of 0.97, 0.97, 0.96 and 0.97, respectively. When a cut off value of 25 mg, 50 mg. 100 mg, and 150 mg for urine protein were used for 2-hour, 4-hours, 8-hour and 12-hour urine collection, a sensitivity of 92.45%, 95.28%, 91.51%, and 96.23% and a specificity of 68.42%, 94.74%, 84.21% and 84.21% were obtained, respectively.Two-hour urine proteins can be used for assessment of proteinuria in preeclampsia instead of gold standard 24-hour urine collection for early diagnosis and better patient compliance.

  14. Comparison of the Current Diagnostic Criterion of HbA1c with Fasting and 2-Hour Plasma Glucose Concentration.

    Science.gov (United States)

    Karnchanasorn, Rudruidee; Huang, Jean; Ou, Horng-Yih; Feng, Wei; Chuang, Lee-Ming; Chiu, Ken C; Samoa, Raynald

    2016-01-01

    To determine the effectiveness of hemoglobin A1c (HbA1c) ≥ 6.5% in diagnosing diabetes compared to fasting plasma glucose (FPG) ≥ 126 mg/dL and 2-hour plasma glucose (2hPG) ≥ 200 mg/dL in a previously undiagnosed diabetic cohort, we included 5,764 adult subjects without established diabetes for whom HbA1c, FPG, 2hPG, and BMI measurements were collected. Compared to the FPG criterion, the sensitivity of HbA1c ≥ 6.5% was only 43.3% (106 subjects). Compared to the 2hPG criterion, the sensitivity of HbA1c ≥ 6.5% was only 28.1% (110 subjects). Patients who were diabetic using 2hPG criterion but had HbA1c STD), female (53.2% versus 38.2%, P = 0.008), leaner (29.7 ± 6.1 versus 33.0 ± 6.6 kg/m(2), P = 0.000005), and less likely to be current smokers (18.1% versus 29.1%, P = 0.02) as compared to those with HbA1c ≥ 6.5%. The diagnostic agreement in the clinical setting revealed the current HbA1c ≥ 6.5% is less likely to detect diabetes than those defined by FPG and 2hPG. HbA1c ≥ 6.5% detects less than 50% of diabetic patients defined by FPG and less than 30% of diabetic patients defined by 2hPG. When the diagnosis of diabetes is in doubt by HbA1c, FPG and/or 2hPG should be obtained. PMID:27597979

  15. Interconversion of Active and Inactive Conformations of Urokinase-Type Plasminogen Activator

    DEFF Research Database (Denmark)

    Liu, Zhuo; Kromann-Hansen, Tobias; Lund, Ida K;

    2012-01-01

    The catalytic activity of serine proteases depends on a salt-bridge between the amino group of residue 16 and the side chain of Asp194. The salt-bridge stabilizes the oxyanion hole and the S1 specificity pocket of the protease. Some serine proteases exist in only partially active forms, in which...... the amino group of residue 16 is exposed to the solvent. Such a partially active state is assumed by a truncated form of the murine urokinase-type plasminogen activator (muPA), consisting of residues 16-243. Here we investigated the allosteric interconversion between partially active states and the...

  16. Synthesis of New Ligands of Urokinase Receptor and New Urotensin-II Derivatives

    OpenAIRE

    Yousif, Ali Munaim

    2015-01-01

    Unit 1: The urokinase-type plasminogen activator receptor (uPAR) is involved in the regulation of cell migration. The uPAR is formed by three domains connected by short linker region. The Ser88-Arg-Ser- Arg-Tyr92 is the minimum chemotactic sequence of uPAR required to induce the same intracellular signaling as result of its binding with formyl peptide receptor (FPRs). With aim to perform a SAR study on this sequence we have synthesized a large peptide library using phosphorylated ...

  17. Urokinase Expression by Tumor Suppressor Protein p53: A Novel Role in mRNA Turnover

    OpenAIRE

    Shetty, Praveenkumar; Velusamy, Thirunavukkarasu; Bhandary, Yashodhar P.; Shetty, Rashmi S.; Liu, Ming-Cheh; Shetty, Sreerama

    2008-01-01

    Lung carcinoma (H1299) cells deficient in p53 (p53−/−) express large amounts of urokinase-type plasminogen activator (uPA) protein and uPA mRNA, and exhibit slower degradation of uPA mRNA than that of p53-expressing nonmalignant Beas2B human airway epithelial cells. Expression of p53 protein in H1299 cells, upon transfection with p53 cDNA, suppressed basal as well as uPA-induced expression of uPA protein in both conditioned media and cell lysates, and decreased the level of steady-state uPA m...

  18. Soluble Urokinase Plasminogen Activator Receptor Levels in Tuberculosis Patients at High Risk for Multidrug Resistance

    OpenAIRE

    Tri Yudani Mardining Raras; Triwahju Astuti; Iin Noor Chozin

    2012-01-01

    The soluble urokinase plasminogen activator receptor (suPAR) has been shown to be a strong prognostic biomarker for tuberculosis (TB). In the present study, the profiles of plasma suPAR levels in pulmonary TB patients at high risk for multidrug resistance were analyzed and compared with those in multidrug resistant (MDR)-TB patients. Forty patients were prospectively included, consisting of 10 MDR-TB patients and 30 TB patients at high risk for MDR, underwent clinical assesment. Plasma suPAR ...

  19. Aberrant glomerular filtration of urokinase-plasminogen activator in nephrotic syndrome leads to amiloride-sensitive plasminogen activation in urine

    DEFF Research Database (Denmark)

    Stæhr, Mette; Buhl, Kristian Bergholt; Andersen, René F;

    2015-01-01

    In nephrotic syndrome, aberrant glomerular filtration of plasminogen and conversion to active plasmin in pre-urine is thought to activate proteolytically ENaC and contribute to sodium retention and edema. The ENaC blocker amiloride is an off-target inhibitor of urokinase-type plasminogen activator...

  20. Soluble urokinase receptor levels in plasma during 5 years of highly active antiretroviral therapy in HIV-1-infected patients

    DEFF Research Database (Denmark)

    Ostrowski, Sisse R; Katzenstein, Terese L; Piironen, Timo;

    2004-01-01

    High blood levels of the soluble urokinase receptor (suPAR) strongly predict increased mortality in human immunodeficiency virus-1 (HIV-1)-infected patients. This study investigated the plasma concentration of suPAR in 29 treatment-naive HIV-1-infected patients during 5 years treatment with highl...

  1. The liberated domain I of urokinase plasminogen activator receptor--a new tumour marker in small cell lung cancer

    DEFF Research Database (Denmark)

    Almasi, Charlotte E; Drivsholm, Lars; Pappot, Helle;

    2013-01-01

    The prognosis of small cell lung cancer (SCLC) remains poor with a 5-year survival rate of 4-6%. In non-small cell lung cancer (NSCLC), high levels of intact and cleaved forms of the receptor for urokinase plasminogen activator (uPAR) are significantly associated with short overall survival. Our...

  2. Serum levels of soluble urokinase plasminogen activator receptor is associated with parasitemia in children with acute Plasmodium falciparum malaria infection

    DEFF Research Database (Denmark)

    Perch, M; Kofoed, Pe; Fischer, Torge;

    2004-01-01

    Serum levels of soluble urokinase plasminogen activator receptor (suPAR) are significantly elevated and of prognostic value in patients suffering from serious infectious diseases such as HIV and tuberculosis. Our objective was to investigate suPAR levels during symptomatic malaria infection and 7...... an important association between suPAR and acute malaria infection in humans....

  3. Soluble urokinase plasminogen activator receptor is a marker of dysmetabolism in HIV-infected patients receiving highly active antiretroviral therapy

    DEFF Research Database (Denmark)

    Andersen, Ove; Eugen-Olsen, Jesper; Kofoed, Kristian;

    2008-01-01

    Circulating soluble urokinase plasminogen activator receptor (suPAR) reflects the immune and pro-inflammatory status of the HIV-infected patient. Highly active antiretroviral therapy (HAART) suppresses suPAR. Independent of the immune response to HAART, suPAR remains elevated in some HIV-infected...

  4. Soluble urokinase receptor is elevated in cerebrospinal fluid from patients with purulent meningitis and is associated with fatal outcome

    DEFF Research Database (Denmark)

    Ostergaard, Christian; Benfield, Thomas; Lundgren, Jens D;

    2004-01-01

    The urokinase-type plasminogen activator system has been suggested to play a pathophysiological role in brain damage. The aim of this study was to evaluate CSF levels of suPAR in 183 patients clinically suspected of having meningitis on admission. Of these, 54 patients were found to have purulent...

  5. Administration of Recombinant Soluble Urokinase Receptor Per Se Is Not Sufficient to Induce Podocyte Alterations and Proteinuria in Mice

    DEFF Research Database (Denmark)

    Cathelin, Dominique; Placier, Sandrine; Ploug, Michael;

    2014-01-01

    Circulating levels of soluble forms of urokinase-type plasminogen activator receptor (suPAR) are generally elevated in sera from children and adults with FSGS compared with levels in healthy persons or those with other types of kidney disease. In mice lacking the gene encoding uPAR, forced increa...

  6. Genetic association of urokinase-type plasminogen activator gene rs2227564 site polymorphism with sporadic Alzheimer's disease in the Han Chinese population

    Institute of Scientific and Technical Information of China (English)

    Xuelian Ji; Longfei Jia; Jianping Jia; Li Qi

    2012-01-01

    A missense C/T polymorphism in exon 6 (the NCBI rsID is rs2227564) of the urokinase-type plasminogen activator gene has been identified as a possible hot spot for Alzheimer's disease risk.The present study analyzed urokinase-type plasminogen gene polymorphisms of rs2227564 with sporadic Alzheimer's disease by PCR-restriction fragment length polymorphism.Results showed that CC,CT and TT genotype distribution frequencies had significant differences between sporadic Alzheimer's disease patients and healthy controls.In-depth analysis of the association between urokinase-type plasminogen gene rs2227564 polymorphisms and sporadic Alzheimer's disease indicated that people with the C-positive genotype CC + CT were at a higher risk for developing sporadic Alzheimer's disease.These results support the contribution of the polymorphisms of rs2227564 in the urokinase-type plasminogen gene to the pathogenesis of sporadic Alzheimer's disease in the Han Chinese population.

  7. Urokinase plasminogen activator receptor affects bone homeostasis by regulating osteoblast and osteoclast function

    DEFF Research Database (Denmark)

    Furlan, Federico; Galbiati, Clara; Jørgensen, Niklas R;

    2007-01-01

    reorganization in mature osteoclasts. INTRODUCTION: Urokinase receptor (uPAR) is actively involved in the regulation of important cell functions, such as proliferation, adhesion, and migration. It was previously shown that the major players in bone remodeling, osteoblasts and osteoclasts, express u...... with other osteoblasts markers. On the resorptive side, the number of osteoclasts formed in vitro from uPAR KO monocytes was decreased. Podosome imaging in uPAR KO osteoclasts revealed a defect in actin ring formation. CONCLUSIONS: The defective proliferation and differentiation of bone cells, coincident......The uPAR and its ligand uPA are expressed by both osteoblasts and osteoclasts. Their function in bone remodeling is unknown. We report that uPAR-lacking mice display increased BMD, increased osteogenic potential of osteoblasts, decreased osteoclasts formation, and altered cytoskeletal...

  8. Urokinase plasminogen activator receptor on invasive cancer cells: A prognostic factor in distal gastric adenocarcinoma

    DEFF Research Database (Denmark)

    Alpizar, Warner Enrique Alpizar; Christensen, Ib Jarle; Santoni-Rugiu, Eric;

    2012-01-01

    Gastric cancer is the second cancer causing death worldwide. The five-year survival for this malignancy is below 25% and few parameters have shown an impact on the prognosis of the disease. The receptor for urokinase plasminogen activator (uPAR) is involved in extracellular matrix degradation...... by mediating cell surface associated plasminogen activation, and its presence on gastric cancer cells is linked to micrometastasis and poor prognosis. Using immunohistochemistry, the prognostic significance of uPAR was evaluated in tissue samples from a retrospective series of 95 gastric cancer patients. u...... association between the expression of uPAR on tumor cells in the peripheral invasion zone and overall survival of gastric cancer patients (HR = 2.16; 95% CI: 1.13-4.14; p = 0.02). Multivariate analysis showed that uPAR immunoreactivity in cancer cells at the invasive front is an independent prognostic factor...

  9. The human receptor for urokinase plasminogen activator. NH2-terminal amino acid sequence and glycosylation variants

    DEFF Research Database (Denmark)

    Behrendt, N; Rønne, E; Ploug, M;

    1990-01-01

    The receptor for human urokinase-type plasminogen activator (u-PA) was purified from phorbol 12-myristate 13-acetate-stimulated U937 cells by temperature-induced phase separation of detergent extracts, followed by affinity chromatography with immobilized diisopropyl fluorophosphate-treated u......, but no N-acetyl-D-galactosamine. Glycosylation is responsible for substantial heterogeneity in the receptor on phorbol ester-stimulated U937 cells, and also for molecular weight variations among various cell lines. The amino acid composition and the NH2-terminal amino acid sequence are reported...... amino-terminal fragment; 2) the identical electrophoretic mobilities observed for cross-linked conjugates, formed between either the purified protein or the u-PA receptor on intact U937 cells and the above ligands; 3) the identity of the apparent molecular weight of the purified protein...

  10. Targeting the autolysis loop of urokinase-type plasminogen activator with conformation-specific monoclonal antibodies

    DEFF Research Database (Denmark)

    Bøtkjær, Kenneth Alrø; Fogh, Sarah; Bekes, Erin C;

    2011-01-01

    PA-specific antibody by shielding the access of plasminogen to the active site. Furthermore, using immunofluorescence, the conformation-specific antibodies mAb-112 and mAb-12E6B10 enabled us to selectively stain pro-uPA or active uPA on the surface of cultured cells. Moreover, in various independent model systems......Tight regulation of serine proteases is essential for their physiological function, and unbalanced states of protease activity have been implicated in a variety of human diseases. One key example is the presence of uPA (urokinase-type plasminogen activator) in different human cancer types......, with high levels correlating with a poor prognosis. This observation has stimulated efforts into finding new principles for intervening with uPA's activity. In the present study we characterize the so-called autolysis loop in the catalytic domain of uPA as a potential inhibitory target. This loop was found...

  11. Soluble Urokinase-Type Plasminogen Activator Receptor Levels in Patients With Schizophrenia

    DEFF Research Database (Denmark)

    Nielsen, Jimmi; Røge, Rasmus; Pristed, Sofie Gry;

    2015-01-01

    BACKGROUND: The etiology of schizophrenia remains largely unknown but alterations in the immune system may be involved. In addition to the psychiatric symptoms, schizophrenia is also associated with up to 20 years reduction in life span. Soluble urokinase-type plasminogen activator receptor (su......PAR) is a protein that can be measured in blood samples and reflects the levels of inflammatory activity. It has been associated with mortality and the development of type 2 diabetes and cardiovascular disease. METHODS: suPAR levels in patients with schizophrenia were compared to healthy controls from the Danish...... Blood Donor Study. SuPAR levels were dichotomized at >4.0 ng/ml, which is considered the threshold for low grade inflammation. A multiple logistic regression model was used and adjusted for age, sex, and current smoking. RESULTS: In total we included 1009 subjects, 105 cases with schizophrenia (10...

  12. Urokinase mediates endothelial cell survival via induction of the X-linked inhibitor of apoptosis protein

    DEFF Research Database (Denmark)

    Prager, Gerald W; Mihaly, Judit; Brunner, Patrick M;

    2008-01-01

    factor kappaB (NF-kappaB) p52 activation. Indeed, blocking NF-kappaB activation by using specific NF-kappaB inhibitors abolished uPA-induced cell survival as it blocked uPA-induced XIAP up-regulation. Furthermore, down-regulating XIAP expression by small interfering RNA (siRNA) significantly reduced u......Urokinase-type plasminogen activator (uPA) additionally elicits a whole array of pro-angiogenic responses, such as differentiation, proliferation, and migration. In this study, we demonstrate that in endothelial cells uPA also protects against apoptosis by transcriptional up-regulation and......PA-dependent endothelial cell survival. This mechanism is also important for VEGF-induced antiapoptosis because VEGF-dependent up-regulation of XIAP was found defective in uPA(-/-) endothelial cells. This led us to conclude that uPA is part of a novel NF-kappaB-dependent cell survival pathway....

  13. Ligand binding alters dimerization and sequestering of urokinase receptors in raft-mimicking lipid mixtures.

    Science.gov (United States)

    Ge, Yifan; Siegel, Amanda P; Jordan, Rainer; Naumann, Christoph A

    2014-11-01

    Lipid heterogeneities, such as lipid rafts, are widely considered to be important for the sequestering of membrane proteins in plasma membranes, thereby influencing membrane protein functionality. However, the underlying mechanisms of such sequestration processes remain elusive, in part, due to the small size and often transient nature of these functional membrane heterogeneities in cellular membranes. To overcome these challenges, here we report the sequestration behavior of urokinase receptor (uPAR), a glycosylphosphatidylinositol-anchored protein, in a planar model membrane platform with raft-mimicking lipid mixtures of well-defined compositions using a powerful optical imaging platform consisting of confocal spectroscopy XY-scans, photon counting histogram, and fluorescence correlation spectroscopy analyses. This methodology provides parallel information about receptor sequestration, oligomerization state, and lateral mobility with single molecule sensitivity. Most notably, our experiments demonstrate that moderate changes in uPAR sequestration are not only associated with modifications in uPAR dimerization levels, but may also be linked to ligand-mediated allosteric changes of these membrane receptors. Our data show that these modifications in uPAR sequestration can be induced by exposure to specific ligands (urokinase plasminogen activator, vitronectin), but not via adjustment of the cholesterol level in the planar model membrane system. Good agreement of our key findings with published results on cell membranes confirms the validity of our model membrane approach. We hypothesize that the observed mechanism of receptor translocation in the presence of raft-mimicking lipid mixtures is also applicable to other glycosylphosphatidylinositol-anchored proteins.

  14. The Prognostic Significance of Soluble Urokinase Plasminogen Activator Receptor in Acute Myeloid Leukemia

    Directory of Open Access Journals (Sweden)

    Nergiz Erkut

    2016-05-01

    Full Text Available Objective: The soluble urokinase plasminogen activator receptor (suPAR is a soluble form of the urokinase plasminogen activator receptor expressed in various immune and cancer cells. The levels of suPAR have been demonstrated to correlate with prognosis in various cancers. This study was intended to investigate serum suPAR levels and their effect on prognosis in patients with acute myeloid leukemia (AML. Materials and Methods: Thirty newly diagnosed patients with AML and 29 healthy individuals were enrolled. Serum suPAR levels were analyzed by enzyme-linked immunosorbent assay. Results: Serum suPAR levels were significantly higher in patients with AML than in healthy individuals (9±5.9 ng/mL and 2.4±1.4 ng/mL, respectively; p<0.001. Positive correlation was determined between suPAR levels and white blood cell counts (p<0.01. Serum suPAR levels were lower in patients who achieved complete response than in patients not achieving complete response (5.5±2.2 ng/mL and 12±6.6 ng/mL, respectively; p<0.001. The median overall survival was longer in patients with serum suPAR levels below 6.71 ng/mL than in those with serum suPAR levels above 6.71 ng/mL (12.6±13.2 months and 1.71±0.6 months, respectively; p=0.02. Multivariate Cox regression analysis showed that suPAR had independent prognostic value (95% confidence interval: 1.029-6.259; p<0.05 in AML. Conclusion: Serum suPAR levels can be used as a prognostic marker in AML.

  15. Activity and expression of urokinase-type plasminogen activator and matrix metalloproteinases in human colorectal cancer

    International Nuclear Information System (INIS)

    Matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), and urokinase-type plasminogen activator (uPA) are involved in colorectal cancer invasion and metastasis. There is still debate whether the activity of MMP-2 and MMP-9 differs between tumors located in the colon and rectum. We designed this study to determine any differences in the expression of MMP-2, MMP-9 and uPA system between colon and rectal cancer tissues. Cancer tissue samples were obtained from colon carcinoma (n = 12) and rectal carcinomas (n = 10). MMP-2 and MMP-9 levels were examined using gelatin zymography and Western blotting; their endogenous inhibitors, tissue inhibitor of metalloproteinase-2 (TIMP-2) and tissue inhibitor of metalloproteinase-1 (TIMP-1), were assessed by Western blotting. uPA, uPAR and PAI-1 were examined using enzyme-linked immunosorbent assay (ELISA). The activity of uPA was assessed by casein-plasminogen zymography. In both colon and rectal tumors, MMP-2, MMP-9 and TIMP-1 protein levels were higher than in corresponding paired normal mucosa, while TIMP-2 level in tumors was significantly lower than in normal mucosa. The enzyme activities or protein levels of MMP-2, MMP-9 and their endogenous inhibitors did not reach a statistically significant difference between colon and rectal cancer compared with their normal mucosa. In rectal tumors, there was an increased activity of uPA compared with the activity in colon tumors (P = 0.0266), however urokinase-type plasminogen activator receptor (uPAR) and plasminogen activator inhibitor-1 (PAI-1) showed no significant difference between colon and rectal cancer tissues. These findings suggest that uPA may be expressed differentially in colon and rectal cancers, however, the activities or protein levels of MMP-2, MMP-9, TIMP-1, TIMP-2, PAI-1 and uPAR are not affected by tumor location in the colon or the rectum

  16. The superoxide scavenger TEMPOL induces urokinase receptor (uPAR expression in human prostate cancer cells

    Directory of Open Access Journals (Sweden)

    Francis Joseph

    2006-06-01

    Full Text Available Abstract There is little understanding of the effect that reactive oxygen metabolites have on cellular behavior during the processes of invasion and metastasis. These oxygen metabolites could interact with a number of targets modulating their function such as enzymes involved in basement membrane dissolution, adhesion molecules involved in motility or receptors involved in proliferation. We investigated the effect of increased scavenging of superoxide anions on the expression of the urokinase receptor (uPAR in PC-3M human prostate cancer cells. Urokinase receptor is a GPI-linked cell surface molecule which mediates multiple functions including adhesion, proliferation and pericellular proteolysis. Addition of the superoxide scavenger 4-hydroxy-2,2,6,6-tetramethylpiperidinyloxy (TEMPOL to PC-3M cultures stimulated expression of uPAR protein peaking between 48 and 72 hours. Cell surface expression of the uPAR was also increased. Surprisingly, uPAR transcript levels increased only slightly and this mild increase did not coincide with the striking degree of protein increase. This disparity indicates that the TEMPOL effect on uPAR occurs through a post-transcriptional mechanism. TEMPOL presence in PC-3M cultures reduced intracellular superoxide-type species by 75% as assayed by NBT dye conversion; however this reduction significantly diminished within hours following TEMPOL removal. The time gap between TEMPOL treatment and peak uPAR protein expression suggests that reduction of reactive oxygen metabolites in prostate cancer cells initiates a multistep pathway which requires several hours to culminate in uPAR induction. These findings reveal a novel pathway for uPAR regulation involving reactive oxygens such as superoxide anion.

  17. The receptor for urokinase regulates TLR2 mediated inflammatory responses in neutrophils.

    Directory of Open Access Journals (Sweden)

    Gang Liu

    Full Text Available The urokinase-type plasminogen activator receptor (uPAR, a glycosylphosphatidylinositol (GPI anchored membrane protein, regulates urokinase (uPA protease activity, chemotaxis, cell-cell interactions, and phagocytosis of apoptotic cells. uPAR expression is increased in cytokine or bacteria activated cell populations, including macrophages and monocytes. However, it is unclear if uPAR has direct involvement in the response of inflammatory cells, such as neutrophils and macrophages, to Toll like receptor (TLR stimulation. In this study, we found that uPAR is required for optimal neutrophil activation after TLR2, but not TLR4 stimulation. We found that the expression of TNF-α and IL-6 induced by TLR2 engagement in uPAR-/- neutrophils was less than that in uPAR+/+ (WT neutrophils. Pretreatment of neutrophils with PI-PLC, which cleaves GPI moieties, significantly decreased TLR2 induced expression of TNF-α in WT neutrophils, but demonstrated only marginal effects on TNF-α expression in PAM treated uPAR-/- neutrophils. IκB-α degradation and NF-κB activation were not different in uPAR-/- or WT neutrophils after TLR2 stimulation. However, uPAR is required for optimal p38 MAPK activation after TLR2 engagement. Consistent with the in vitro findings that uPAR modulates TLR2 engagement induced neutrophil activation, we found that pulmonary and systemic inflammation induced by TLR2, but not TLR4 stimulation is reduced in uPAR-/- mice compared to WT counterparts. Therefore, our data suggest that neutrophil associated uPAR could be a potential target for treating acute inflammation, sepsis, and organ injury related to severe bacterial and other microbial infections in which TLR2 engagement plays a major role.

  18. Outcome evaluation of intra-arterial infusion of urokinase for acute ischemic stroke

    International Nuclear Information System (INIS)

    To evaluate the results of intra-arterial urokinase thrombolysis in cases of acute ischemic stroke and to define the factors affecting prognosis. Forty-eight patients with angiographically proven occlusion of the intracranial arteries were treated with local intra-arterial infusion of urokinase within six hours of the onset of symptoms. Neurologic status was evaluated on admission and on discharge using the NIH (National Institute of Health) stroke scale score (SSS). When the SSS decreased by at least four points, this was considered indicative of an improved clinical outcome. Complete recanalization was achieved in 17/48 patients (35%), including 8 of 13 (62%) with occlusion of the vertebrobasilar artery (VBA), 9 of 20 (45%) with occlusion of the middle cerebral artery (MCA), and none of 15 with occlusion of the internal carotid artery (ICA). Neurologic status improved in 12 (60%) of patients with MCA occlusion, in five (38%) of those with VBA occlusion and in three (20%) of those with ICA occlusion (p less than 0.005). Patients in whom occluded MCA was completely recanalized showed greater clinical improvement than those with partial or no recanalization (p less than 0.05). The overall mortality rate was 21%, 43% (9/21) in patients in whom CT revealed signs of early infarct, but only 4% (1/27) in those without this sign (p less than 0.05). The mortality rate of patients with parenchymal hematoma (4/5) was higher than that of those with hemorrhagic infarct (3/9) or without hemorrhage (3/34) (p less than 0.005). In patients in whom occluded MCA was completely recanalized, the clinical outcome was better, while patients with VBA occlusion did not benefit from recanalization. The presence on CT scans of signs of early infarct and of parenchymal hematoma after thrombolysis correlated with a high mortality rate. (author)

  19. Outcome evaluation of intra-arterial infusion of urokinase for acute ischemic stroke

    Energy Technology Data Exchange (ETDEWEB)

    Shi, Hai Bin [First Affiliated Hospital of Nanjing Medical University, Nanjing (China); Suh, Dae Chul; Lim, Soo Mee [Asan Medical Center, College of Medicine, University of Ulsan, Seoul (Korea, Republic of); And Others

    2000-06-01

    To evaluate the results of intra-arterial urokinase thrombolysis in cases of acute ischemic stroke and to define the factors affecting prognosis. Forty-eight patients with angiographically proven occlusion of the intracranial arteries were treated with local intra-arterial infusion of urokinase within six hours of the onset of symptoms. Neurologic status was evaluated on admission and on discharge using the NIH (National Institute of Health) stroke scale score (SSS). When the SSS decreased by at least four points, this was considered indicative of an improved clinical outcome. Complete recanalization was achieved in 17/48 patients (35%), including 8 of 13 (62%) with occlusion of the vertebrobasilar artery (VBA), 9 of 20 (45%) with occlusion of the middle cerebral artery (MCA), and none of 15 with occlusion of the internal carotid artery (ICA). Neurologic status improved in 12 (60%) of patients with MCA occlusion, in five (38%) of those with VBA occlusion and in three (20%) of those with ICA occlusion (p less than 0.005). Patients in whom occluded MCA was completely recanalized showed greater clinical improvement than those with partial or no recanalization (p less than 0.05). The overall mortality rate was 21%, 43% (9/21) in patients in whom CT revealed signs of early infarct, but only 4% (1/27) in those without this sign (p less than 0.05). The mortality rate of patients with parenchymal hematoma (4/5) was higher than that of those with hemorrhagic infarct (3/9) or without hemorrhage (3/34) (p less than 0.005). In patients in whom occluded MCA was completely recanalized, the clinical outcome was better, while patients with VBA occlusion did not benefit from recanalization. The presence on CT scans of signs of early infarct and of parenchymal hematoma after thrombolysis correlated with a high mortality rate. (author)

  20. Activation of human pro-urokinase by unrelated proteases secreted by Pseudomonas aeruginosa.

    Science.gov (United States)

    Beaufort, Nathalie; Seweryn, Paulina; de Bentzmann, Sophie; Tang, Aihua; Kellermann, Josef; Grebenchtchikov, Nicolai; Schmitt, Manfred; Sommerhoff, Christian P; Pidard, Dominique; Magdolen, Viktor

    2010-06-15

    Pathogenic bacteria, including Pseudomonas aeruginosa, interact with and engage the host plasminogen (Plg) activation system, which encompasses the urokinase (uPA)-type Plg activator, and is involved in extracellular proteolysis, including matrilysis and fibrinolysis. We hypothesized that secreted bacterial proteases might contribute to the activation of this major extracellular proteolytic system, thereby participating in bacterial dissemination. We report that LasB, a thermolysin-like metalloprotease secreted by Ps. aeruginosa, converts the human uPA zymogen into its active form (kcat=4.9 s-1, Km=8.9 microM). Accordingly, whereas the extracellular secretome from the LasB-expressing pseudomonal strain PAO1 efficiently activates pro-uPA, the secretome from the isogenic LasB-deficient strain PDO240 is markedly less potent in pro-uPA activation. Still, both secretomes induce some metalloprotease-independent activation of the human zymogen. The latter involves a serine protease, which we identified via both recombinant protein expression in Escherichia coli and purification from pseudomonal cultures as protease IV (PIV; kcat=0.73 s-1, Km=6.2 microM). In contrast, neither secretomes nor the pure proteases activate Plg. Along with this, LasB converts Plg into mini-Plg and angiostatin, whereas, as reported previously, it processes the uPA receptor, inactivates the plasminogen activator inhibitor 1, and activates pro-matrix metalloproteinase 2. PIV does not target these factors at all. To conclude, LasB and PIV, although belonging to different protease families and displaying quite different substrate specificities, both activate the urokinase-type precursor of the Plg activation cascade. Direct pro-uPA activation, as also reported for other bacterial proteases, might be a frequent phenomenon that contributes to bacterial virulence. PMID:20337595

  1. Sustainable urban regime adjustments

    DEFF Research Database (Denmark)

    Quitzau, Maj-Britt; Jensen, Jens Stissing; Elle, Morten;

    2013-01-01

    The endogenous agency that urban governments increasingly portray by making conscious and planned efforts to adjust the regimes they operate within is currently not well captured in transition studies. There is a need to acknowledge the ambiguity of regime enactment at the urban scale. This directs......, inspired by Actor-Network Theory to demonstrate that regime incumbent actors can induce gradual regime adjustments at the urban scale. This is done through a case study of an urban development project, where the Municipality of Egedal in Denmark has successfully promoted energy efficient buildings through...

  2. Interleukin-6 infusion during human endotoxaemia inhibits in vitro release of the urokinase receptor from peripheral blood mononuclear cells

    DEFF Research Database (Denmark)

    Ostrowski, S R; Plomgaard, P; Fischer, C P;

    2005-01-01

    Leucocyte expression of the urokinase receptor [urokinase-type plasminogen activator receptor (uPAR)] is regulated by inflammatory mediators. This study investigated the in vivo effect of endotoxin, interleukin (IL)-6 and tumour necrosis factor (TNF)-alpha on uPAR-release in vivo and in vitro in...... humans. Healthy subjects received intravenous endotoxin injection [high-dose, 2 ng/kg (n=8) and low-dose, 0.06 ng/kg (n=7)], coadministration of 0.06 ng/kg endotoxin and 3 h recombinant human (rh)IL-6 infusion (n=7) or 3 h infusion of rhIL-6 (n=6), rhTNF-alpha (n=6) or NaCl (n=5). Soluble uPAR (su...

  3. Localization of urokinase-type plasminogen activator in stromal cells in adenocarcinomas of the colon in humans.

    OpenAIRE

    Grøndahl-Hansen, J.; Ralfkiaer, E; Kirkeby, L. T.; P. Kristensen; Lund, L. R.; Danø, K

    1991-01-01

    Human colon adenocarcinomas and adjacent normal colon tissues were stained immunohistochemically with three different monoclonal antibodies and one preparation of polyclonal antibodies against each of the two plasminogen activators, uPA (urokinase type) and tPA (tissue type). The staining patterns seen with the respective sets of antibodies were identical. In all of 10 cases, staining for uPA in the normal colon tissue was confined to scattered fibroblastlike cells in the lamina propria. Othe...

  4. Quantitative PET of human urokinase-type plasminogen activator receptor with 64Cu-DOTA-AE105

    DEFF Research Database (Denmark)

    Persson, Morten; Madsen, Jacob; Østergaard, Søren;

    2012-01-01

    Expression levels of the urokinase-type plasminogen activator receptor (uPAR) represent an established biomarker for poor prognosis in a variety of human cancers. The objective of the present study was to explore whether noninvasive PET can be used to perform a quantitative assessment of expression...... levels of uPAR across different human cancer xenograft models in mice and to illustrate the clinical potential of uPAR PET in future settings for individualized therapy....

  5. The structure and function of the urokinase receptor, a membrane protein governing plasminogen activation on the cell surface

    DEFF Research Database (Denmark)

    Behrendt, N; Rønne, E; Danø, K

    1995-01-01

    Proteolytic degradation processes, resulting from the activation of plasminogen by the specific enzyme, urokinase plasminogen activator (uPA), take place in the extracellular matrix during cancer invasion as well as in tissue remodelling under certain normal-physiological conditions. The uPA rece...... with the continuous identification of inhibitory reagents, this knowledge should open the possibility to interfere with the resulting, degradative events....

  6. Urokinase-targeted recombinant bacterial protein toxins-a rationally designed and engineered anticancer agent for cancer therapy

    Institute of Scientific and Technical Information of China (English)

    Yizhen LIU; Shi-Yan LI

    2009-01-01

    Urokinase-targeted recombinant bacterial protein toxins are a sort of rationally designed and engineered anticancer recombinant fusion proteins representing a novel class of agents for cancer therapy.Bacterial protein toxins have long been known as the primary virulence factor(s) for a variety of pathogenic bacteria and are the most powerful human poisons.On the other hand,it has been well documented that urokinase-type plasminogen activator (uPA) and urokinase plasminogen activator receptor (uPAR),making up the uPA system,are overexpressed in a variety of human tumors and tumor cell lines.The expression of uPA system is highly correlated with tumor invasion and metastasis.To exploit these characteristics in the design of tumor cell-selective cytotoxins,two prominent bacterial protein toxins,i.e.,the diphtheria toxin and anthrax toxin are deliberately engineered through placing a sequence targeted specifically by the uPA system to form anticancer recombinant fusion proteins.These uPA system-targeted bacterial protein toxins are activated selectively on the surface of uPA systemexpressing tumor cells,thereby killing these cells.This article provides a review on the latest progress in the exploitation of these recombinant fusion proteins as potent tumoricidal agents.It is perceptible that the strategies for cancer therapy are being innovated by this novel therapeutic approach.

  7. 纳米尿激酶的性状研究%Characterization of urokinase chitosan nanoparticles

    Institute of Scientific and Technical Information of China (English)

    金海将; 张皓; 张柏根; 孙敏莉

    2011-01-01

    背景:尿激酶半衰期短,需持续大量给药,并发症也不小,故有必要研制具有缓释作用的溶栓药物.目的:了解包载尿激酶的水溶性壳聚糖纳米粒子的性状.方法:将壳聚糖和三聚磷酸钠以离子凝集法制备尿激酶纳米粒子后,用透射电镜观察其形态,采用粒径仪测纳米粒子粒径,酶标仪比色法测粒子包封率,纳米粒子冻干法测其载药量,并检测体内外纳米粒子缓释特性.结果与结论:当壳聚糖、三聚磷酸钠、尿激酶的质量比为7∶1∶1时,不仅溶液稳定,形成的纳米粒子粒径小,而且包封率和载药量均合适.制备出包封率最高达94.8%的尿激酶纳米粒子,载药量为14.5%,此时平均粒径236 nm,透射电镜观察示粒子形态较规则,呈球形;粒子具有较好的缓释性能;表明将尿激酶包被于纳米粒子中,避免了消化酶的直接作用,延长了半衰期,不仅在体内能保持较长时间的活性,而且具有明显的缓释效果.%BACKGROUND: Because urokinase has a short half-life, and has a sustained large dose, resulting in in negligible complications,it is necessary to develops low- release thrombolytic drugs .OBJECTIVE: To study the characterization of urokinase chitosan nanoparticles.METHODS: The nanoparticles were prepared via the self-assembly of chitosan and sodium tripolyphosphate. Themorphololywas observed by transmission electron microscopy (TEM). Particle size was measured with particle size instrument Theencapsulation efficiency was tested by ELISA Reader. Drug loading efficiency was measured by the way of weighing lyophiizedpowder. Release characteristics of the nanoparticles were investigated both in vitro and in vivo.RESULT SAND CONCLUSION: When the mass ratio of chitosan, sodium trip olyphosph ate, and ur ok in as was 7:1:1, the solutionwas stable, the nanoparticle size was small, and drug encapsulation efficiency and loading efficiency were appropriate. When thehighest encapsulation

  8. Measuring autocratic regime stability

    Directory of Open Access Journals (Sweden)

    Joseph Wright

    2016-01-01

    Full Text Available Researchers measure regime stability in autocratic contexts using a variety of data sources that capture distinct concepts. Often this research uses concepts developed for the study of democratic politics, such as leadership change or institutionalized authority, to construct measures of regime breakdown in non-democratic contexts. This article assesses whether the measure a researcher chooses influences the results they obtain by examining data on executive leadership, political authority, and autocratic regimes. We illustrate the conceptual differences between these variables by extending recent studies in the literature on the political consequences of non-tax revenue and unearned foreign income.

  9. Elevated soluble urokinase plasminogen activator receptor (suPAR) predicts mortality in Staphylococcus aureus bacteremia

    DEFF Research Database (Denmark)

    Mölkänen, T; Ruotsalainen, E; Thorball, C W;

    2011-01-01

    are scarce. To elucidate the role of suPAR in a common bacteremic infection, the serum suPAR levels in 59 patients with Staphylococcus aureus bacteremia (SAB) were measured using the suPARnostic ELISA assay and associations to 1-month mortality and with deep infection focus were analyzed. On day three, after......The soluble form of urokinase-type plasminogen activator receptor (suPAR) is a new inflammatory marker. High suPAR levels have been shown to associate with mortality in cancer and in chronic infections like HIV and tuberculosis, but reports on the role of suPAR in acute bacteremic infections......PAR levels as compared to patients with no deep infection focus. suPAR was found to be prognostic for mortality in receiver operator characteristic (ROC) curve analysis, which was not observed for serum C-reactive protein (CRP); the area under the curve (AUC) for suPAR was 0.754 (95% confidence interval [CI...

  10. Identification of a Novel Nucleus Protein Involved in the Regulation of Urokinase in 95D Cells

    Institute of Scientific and Technical Information of China (English)

    Chang TONG; Li TAN; Ping LI; Yun-Song ZHU

    2005-01-01

    The urokinase-type plasminogen activator (uPA) plays an important role in cellular invasion.By using the downstream part of a 74 bp DNA region called the cooperation mediator (COM) of the uPA promoter as a bait sequence in the yeast one-hybrid screen, a gene called PBK1 was previously cloned from the cDNA library of the 95D lung cancer cell strain. In this study, the intracellular distribution of PBK1 was studied by using the transient transfection of pEGFP-C3-PBK1, and PBK1 was found to be localized in the nucleus. Co-transfection of pEGFP-C3-PBK1 and the deletion mutants of the pGL3-uPA promoter indicated that PBK1 can increase the uPA promoter activity by about 25% and this effect is uPA enhancer-dependent.Western blotting and Enzyme-linked immunoadsordent assay further confirmed that PBK1 can upregulate the expression of uPA. Our results suggest that PBK1 is involved in the regulation of uPA expression, which might provide a new clue to further understanding the regulation mechanism of uPA expression.

  11. Involvement of urokinase-type plasminogen activator system in cancer: an overview.

    Science.gov (United States)

    Mekkawy, Ahmed H; Pourgholami, Mohammad H; Morris, David L

    2014-09-01

    Currently, there are several studies supporting the role of urokinase-type plasminogen activator (uPA) system in cancer. The association of uPA to its receptor triggers the conversion of plasminogen into plasmin. This process is regulated by the uPA inhibitors (PAI-1 and PAI-2). Plasmin promotes degradation of basement membrane and extracellular matrix (ECM) components as well as activation of ECM latent matrix metalloproteases. Degradation and remodeling of the surrounding tissues is crucial in the early steps of tumor progression by facilitating expansion of the tumor mass, release of tumor growth factors, activation of cytokines as well as induction of tumor cell proliferation, migration, and invasion. Hence, many tumors showed a correlation between uPA system component levels and tumor aggressiveness and survival. Therefore, this review summarizes the structure of the uPA system, its contribution to cancer progression, and the clinical relevance of uPA family members in cancer diagnosis. In addition, the review evaluates the significance of uPA system in the development of cancer-targeted therapies.

  12. Soluble urokinase plasminogen activator receptor as a marker for use of antidepressants.

    Directory of Open Access Journals (Sweden)

    Eva Haastrup

    Full Text Available OBJECTIVES: Inflammation is involved in the pathogenesis of depression. A few cross-sectional population-based studies have found that depression is associated with increased levels of inflammatory markers. Soluble urokinase plasminogen activation receptor (suPAR is known to be a stable marker for inflammation. We investigated the bidirectional association between suPAR levels and use of antidepressants. METHODS: suPAR level was measured in 9305 blood donors and analysed in relation to 5-years follow-up data on purchase of antidepressants and hospital diagnoses of depression from a nationwide Danish register. RESULTS: For men and women without prior use of antidepressants we found a significantly higher risk for incident use of antidepressants with higher suPAR values. For men, the risk of first use of antidepressants increased by 72% from the 1st to the 4th quartile (HR = 1.72, 95% CI: 1.11-2.69. For women, it increased by 108% from the 1st to the 4th quartile (HR = 2.08, 95% CI: 1.45-2.98. Previous use of antidepressants was also significantly associated with higher suPAR levels (p = 0.002. CONCLUSIONS: High suPAR levels are associated with an increased risk for both previous and future use of antidepressants in healthy men and women. High suPAR are also associated with increased risk for a hospital diagnosis of depression.

  13. Soluble urokinase plasminogen activator receptor (suPAR) in acute care

    DEFF Research Database (Denmark)

    Rasmussen, Line Jee Hartmann; Ladelund, Steen; Haupt, Thomas Huneck;

    2016-01-01

    . METHODS: This registry-based retrospective cohort study included 4343 consecutively admitted patients from the Acute Medical Unit at a large Danish university hospital. Time to readmission and death were analysed by multiple Cox regression. Results were reported as HRs for 30-day and 90-day follow......OBJECTIVE: Soluble urokinase plasminogen activator receptor (suPAR) is an inflammatory biomarker associated with presence and progression of disease and with increased risk of mortality. We aimed to evaluate the unspecific biomarker suPAR as a prognostic marker in patients admitted to acute care......-up. RESULTS: During 30-day follow-up, 782 patients (18.0%) were readmitted and 224 patients (5.2%) died. Comparing 30-day readmission and mortality between patients in the highest and lowest suPAR quartiles yielded HRs of 2.11 (95% CI 1.70 to 2.62) and 4.11 (95% CI 2.46 to 6.85), respectively, when adjusting...

  14. [Preparation of novel magnetic dextran affinity adsorbents and their application to purify urokinase].

    Science.gov (United States)

    Dong, Y S; Liang, F; Yu, X Y; Guo, L A; Chang, J H

    2001-01-01

    The reverse phase suspension and embedment technique were adopted to prepare magnetic dextran microsphere (MDMS). The dispersion medium was mixture of some organic solvents. Span-80 was used as stabilizer. The aqueous dextran with magnetic fluid was suspended in dispersion medium with epichlorohydrin as cross-linking reagent. The mixture was stirred for 30 minutes at room temperature and then heated at 70 degrees C for 4 hours, MDMS was thus obtained. MDMS was activated by epichlorohydrin on which 6-aminohexanoic acid, glycine or ethylene diamine was bonded as spacers. Then it was coupled with p-aminobenzamide, L-arginine methyl ester or guanidohexanoic acid and five magnetic affinity adsorbents were prepared. The MDMS was polydisperse particles with the size of 50-300 meshes and the content of Fe3O4 was about 6.2 per cent in the MDMS. Influence of some parameters such as viscosity and density of organic phase, the volume ratio of organic and aqueous phase, the quantity of surfactant and stirring speed on preparing MDMS was studied. Magnetic affinity adsorbents were used to purify crude urokinase in a bath mode and the effect of coupling reagents and ligands on results of purification was discussed. The bioactivity recovery was 40.0 to 60.7 per cent, the purification-fold was between 14.9 and 32.8, and the adsorptive capacity varies from 89 mg to 121 mg per milliliter of adsorbent. PMID:12541840

  15. EXPRESSION AND SIGNIFICANCE OF UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN BREAST CANCER

    Institute of Scientific and Technical Information of China (English)

    XIAO Jiping; ZHANG Guangde; XIA Wenhua; CHENG Deji

    1999-01-01

    Objective: To study the expression and clinical significance of urokinase-type plasminogen activator (uPA) in breast cancer. Methods: Applying streptavidin-biotin complex (SABC) immunohistochemical technique, expression of uPA was studied in 100 patients with primary breast cancer. Results: There were 55 patients with high uPA expression, and 45 with lower expression. There was significant correlation between uPA expression and TNM stage, lymph node status, and the tumor size. Neither age, menopausal status, nor ER status was significantly related with level of uPA expression. The patients with high expression of uPA had significantly shorter disease-free survival (DFS)and overall survival (OS) than did those with low expression of uPA. Univariate analysis showed that uPA as a prognostic factor was of similar magnitude to lymph node status and TNM stage, but stronger than that of ER status and tumor size. UPA was an independent prognostic factor affecting disease-free survival and overall survival. Conclusion: uPA appears to be a strong and independent biologic marker for predicting prognosis of breast cancer.

  16. Soluble Urokinase Receptors in Focal Segmental Glomerulosclerosis: A Review on the Scientific Point of View

    Science.gov (United States)

    Saleem, Moin A.; Meijers, Björn

    2016-01-01

    Focal segmental glomerulosclerosis (FSGS) is one of the primary glomerular disorders in both children and adults which can progress to end-stage renal failure. Although there are genetic and secondary causes, circulating factors have also been regarded as an important factor in the pathogenesis of FSGS, because about 40% of the patients with FSGS have recurrence after renal transplantation. Soluble urokinase-type plasminogen activator receptor (suPAR) is a soluble form of uPAR, which is a membrane-bound protein linked to GPI in various immunologically active cells, including podocytes. It has recently been suggested as a potential circulating factor in FSGS by in vitro podocyte experiments, in vivo mice models, and human studies. However, there have also been controversies on this issue, because subsequent studies showed conflicting results. suPAR levels were also increased in patients with other glomerular diseases and were inversely correlated with estimated glomerular filtration rate. Nevertheless, there has been no balanced review on this issue. In this review, we compare the conflicting data on the involvement of suPAR in the pathogenesis of FSGS and shed light on interpretation by taking into account many points and the potential variables and confounders influencing serum suPAR levels.

  17. Soluble Urokinase Receptors in Focal Segmental Glomerulosclerosis: A Review on the Scientific Point of View

    Directory of Open Access Journals (Sweden)

    Andreas Kronbichler

    2016-01-01

    Full Text Available Focal segmental glomerulosclerosis (FSGS is one of the primary glomerular disorders in both children and adults which can progress to end-stage renal failure. Although there are genetic and secondary causes, circulating factors have also been regarded as an important factor in the pathogenesis of FSGS, because about 40% of the patients with FSGS have recurrence after renal transplantation. Soluble urokinase-type plasminogen activator receptor (suPAR is a soluble form of uPAR, which is a membrane-bound protein linked to GPI in various immunologically active cells, including podocytes. It has recently been suggested as a potential circulating factor in FSGS by in vitro podocyte experiments, in vivo mice models, and human studies. However, there have also been controversies on this issue, because subsequent studies showed conflicting results. suPAR levels were also increased in patients with other glomerular diseases and were inversely correlated with estimated glomerular filtration rate. Nevertheless, there has been no balanced review on this issue. In this review, we compare the conflicting data on the involvement of suPAR in the pathogenesis of FSGS and shed light on interpretation by taking into account many points and the potential variables and confounders influencing serum suPAR levels.

  18. TFRC and ACTB as the best reference genes to quantify Urokinase Plasminogen Activator in breast cancer

    Directory of Open Access Journals (Sweden)

    Abdoli Nasrin

    2011-06-01

    Full Text Available Abstract Background Biomedical researchers have long looked for ways to diagnose and treat cancer patients at the early stages through biomarkers. Although conventional techniques are routinely applied in the detection of biomarkers, attitudes towards using Real-Time PCR techniques in detection of many biomarkers are increasing. Normalization of quantitative Real-Time PCR is necessary to validate non-biological alteration occurring during the steps of RNA quantification. Selection of variably expressed housekeeping genes (HKs will affect the validity of the data. The aim of the present study was to identify uniformly expressed housekeeping genes in order to use in the breast cancer gene expression studies. Urokinase Plasminogen Activator was used as a gene of interest. Findings The expression of six HKs (TFRC, GUSB, GAPDH, ACTB, HPRT1 and RPLP0 was investigated using geNorm and NormFinder softwares in forty breast tumor, four normal and eight adjacent tissues. RPLP0 and GAPDH revealed maximum M value, while TFRC demonstrated lowest M value. Conclusions In the present study the most and the least stable genes were TFRC and RPLP0 respectively. TFRC and ACTB were verified as the best combination of two genes for breast cancer quantification. The result of this study shows that in each gene expression analysis HKs selection should be done based on experiment conditions.

  19. Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple myeloma progression.

    Science.gov (United States)

    Khan, Rehan; Gupta, Nidhi; Kumar, Raman; Sharma, Manoj; Kumar, Lalit; Sharma, Alpana

    2014-06-01

    Multiple myeloma (MM) represents a B cell malignancy, characterized by a monoclonal proliferation of malignant plasma cells. Interactions between tumor cells and extracellular matrix (ECM) are of importance for tumor invasion and metastasis. Protein levels of urokinase plasminogen activator (uPA) and fibulin 1, nidogen and laminin in plasma and serum respectively and mRNA levels of these molecules in peripheral blood mononuclear cells were determined in 80 subjects by using ELISA and quantitative PCR and data was analyzed with severity of disease. Pearson correlation was determined to observe interrelationship between different molecules. A statistical significant increase for ECM proteins (laminin, nidogen and fibulin 1) and uPA at circulatory level as well as at mRNA level was observed compared to healthy controls. The levels of these molecules in serum might be utilized as a marker of active disease. Significant positive correlation of all ECM proteins with uPA was found and data also correlates with severity of disease. Strong association found between ECM proteins and uPA in this study supports that there might be interplay between these molecules which can be targeted. This study on these molecules may help to gain insight into processes of growth, spread, and clinical behavior of MM.

  20. Electroanalysis of pM-levels of urokinase plasminogen activator in serum by phosphorothioated RNA aptamer.

    Science.gov (United States)

    Jarczewska, Marta; Kékedy-Nagy, László; Nielsen, Jesper S; Campos, Rui; Kjems, Jørgen; Malinowska, Elżbieta; Ferapontova, Elena E

    2015-06-01

    Protein biomarkers of cancer allow a dramatic improvement in cancer diagnostics as compared to the traditional histological characterisation of tumours by enabling a non-invasive analysis of cancer development and treatment. Here, an electrochemical label-free assay for urokinase plasminogen activator (uPA), a universal biomarker of several cancers, has been developed based on the recently selected uPA-specific fluorinated RNA aptamer, tethered to a gold electrode via a phosphorothioated dA tag, and soluble redox indicators. The binding properties of the uPA-aptamer couple and interference from the non-specific adsorption of bovine serum albumin (BSA) were modulated by the electrode surface charge. A nM uPA electroanalysis at positively charged surfaces, complicated by the competitive adsorption of BSA, was tuned to the pM uPA analysis at negative surface charges of the electrode, being improved in the presence of negatively charged BSA. The aptamer affinity for uPA displayed via the binding/dissociation constant relationship correspondingly increased, ca. three orders of magnitude, from 0.441 to 367. Under optimal conditions, the aptasensor allowed 10(-12)-10(-9) M uPA analysis, also in serum, being practically useful for clinical applications. The proposed strategy for optimization of the electrochemical protein sensing is of particular importance for the assessment and optimization of in vivo protein ligand binding by surface-tethered aptamers.

  1. Endovascular Treatment of Left Iliofemoral Deep Vein Thrombosis Using Urokinase Thrombolysis and Adjunctive Aspiration Thrombectomy

    Energy Technology Data Exchange (ETDEWEB)

    Suh, Sang Hyun; Lee, Do Yun; Won, Jong Yun [Yonsei University College of Medicine, Seoul (Korea, Republic of)

    2010-02-15

    To evaluate the efficacy of adjunctive aspiration thrombectomy for the treatment of iliofemoral deep vein thrombosis (DVT). 24 patients (9 males and 15 females; mean age, 53 years), treated by aspiration thrombectomy were enrolled in this study. The day after undergoing urokinase (UK) thrombolysis, any residual thrombus over a long segment was treated by aspiration thrombectomy using a 12 Fr long sheath. Residual short-segment (< 10 cm) iliac vein thrombus and/or stenosis were treated with a stent. The evaluation of venous patency was conducted by color Doppler ultrasonography, venography and/or computed tomography. The technical and clinical success rates were 100% and 92%, respectively. Twenty-three patients were treated by UK thrombolysis and iliac stent. The overall patency rate at 1, 2 and 3 years was 85%, 82% and 81%, respectively. Over the course of the follow-up period, occlusion was observed in 4 cases (1 acute and 3 chronic cases). Periprocedural complication occurred in 4 cases (17%) in the form of a minimal hematoma or pain on the puncture site as well as a case of pulmonary embolism at one month after treatment. The adjunctive aspiration thrombectomy with conventional thrombolysis and stent placement can be an effective and safe method in the treatment of left iliofemoral DVT

  2. THE INFLUENCED FLOW REGIMES

    Directory of Open Access Journals (Sweden)

    Gavril PANDI

    2011-03-01

    Full Text Available The influenced flow regimes. The presence and activities ofhumanity influences the uniform environmental system, and in this context, therivers water resources. In concordance with this, the natural runoff regime suffersbigger and deeper changes. The nature of these changes depending on the type anddegree of water uses. The multitude of the use cause different types of influence,whit different quantitative aspects. In the same time, the influences havequalitative connotations, too, regarding to the modifications of the yearly watervolume runoff. So the natural runoff regime is modified. After analyzing thedistribution laws of the monthly runoff, there have been differenced four types ofinfluenced runoff regimes. In the excess type the influenced runoff is bigger thanthe natural, continuously in the whole year. The deficient type is characterized byinverse rapports like the first type, in the whole year. In the sinusoidal type, theinfluenced runoff is smaller than the natural in the period when the water isretained in the lake reservoirs, and in the depletion period the situation inverts. Atthe irregular type the ratio between influenced and natural runoff is changeable ina random meaner monthly. The recognition of the influenced regime and the gradeof influence are necessary in the evaluation and analysis of the usable hydrologicalriver resources, in the flood defence activities, in the complex scheme of thehydrographic basins, in the environment design and so on.

  3. Structure, function and expression on blood and bone marrow cells of the urokinase-type plasminogen activator receptor, uPAR

    DEFF Research Database (Denmark)

    Plesner, T; Behrendt, N; Ploug, M

    1997-01-01

    Several important functions have been assigned to the receptor for urokinase-type plasminogen activator, uPAR. As implied by the name, uPAR was first identified as a high affinity cellular receptor for urokinase plasminogen activator (uPA). It mediates the binding of the zymogen, pro-uPA, to the...... shown that urokinase-type plasminogen activator (uPAR) is expressed as a differentiation antigen on cells of the myelomonocytic lineage and as an activation antigen on monocytes and T lymphocytes. Neutrophils contain intracellular reservoirs of uPAR that are translocated to the plasma membrane upon...... activation, and neutrophils from patients with the rare blood disease paroxysmal nocturnal hemoglobinuria (PNH) that fail to express glycosyl-phosphatidylinositol-anchored proteins including uPAR, show a very significantly reduced transmigration over an endothelial barrier. Cell-associated plasminogen...

  4. World Nonproliferation Regime

    Institute of Scientific and Technical Information of China (English)

    Ouyang Liping; Wu Xingzuo

    2007-01-01

    2006 witnessed an intense struggle between nuclear proliferation and nonproliferation. Iran's nuclear issue and North Korea's nuclear test have cast a deep shadow over the current international nonproliferation regime. The international contest for civil nuclear development became especially fierce as global energy prices went up. Such a situation , to some extent, accelerated the pace of nuclear proliferation. Furthermore, the existing international nonproliferation regime, based upon the Nuclear Nonproliferation Treaty (NPT), was affected by loopholes, and the U.S. failed in its ambition to unite other forces to mend fences. The international community needs to come up with a comprehensive and long-term strategy to meet the demand for an effective future nonproliferation regime in a healthy nuclear order.

  5. Supply regimes in fisheries

    DEFF Research Database (Denmark)

    Nielsen, Max

    2006-01-01

    -economic supply model with mesh sizes is developed. It is found that in the presence of realistic management schemes, the supply curves are close to vertical in the relevant range. Also, the supply curve under open access with mesh size limitations is almost vertical in the relevant range, owing to constant......Supply in fisheries is traditionally known for its backward bending nature, owing to externalities in production. Such a supply regime, however, exist only for pure open access fisheries. Since most fisheries worldwide are neither pure open access, nor optimally managed, rather between the extremes......, the traditional understanding of supply regimes in fisheries needs modification. This paper identifies through a case study of the East Baltic cod fishery supply regimes in fisheries, taking alternative fisheries management schemes and mesh size limitations into account. An age-structured Beverton-Holt based bio...

  6. Dimagrire in regime mediatico

    OpenAIRE

    Boutaud, Jean-Jacques

    2012-01-01

    publication en italien : BOUTAUD Jean-Jacques, « Dimagrire in regime mediatico », Dietetica e semiotica. Regimi di Senso, a cura di Dario Mangano e Gianfranco Marrone, Mimesis Edizioni, Milano, 2013, p. 19-38 Il titolo può leggersi in molti modi. Per esempio: dietetica e semiotica sono due regimi di senso, uno alimentare e l'altro comunicativo, con pari dignità, dunque da paragonare fra loro. Oppure: grazie alla semiotica la dietetica appare, non più come un regime alimentare, ma come un r...

  7. Cellular receptor for urokinase plasminogen activator. Carboxyl-terminal processing and membrane anchoring by glycosyl-phosphatidylinositol

    DEFF Research Database (Denmark)

    Ploug, M; Rønne, E; Behrendt, N;

    1991-01-01

    The cellular receptor for human urokinase-type plasminogen activator (u-PAR) is shown by several independent criteria to be a true member of a family of integral membrane proteins, anchored to the plasma membrane exclusively by a COOH-terminal glycosyl-phosphatidylinositol moiety. 1) Amino acid...... acid compositions derived from cDNA sequence and amino acid analysis shows that a polypeptide of medium hydrophobicity is excised from the COOH terminus of the nascent u-PAR. A similar proteolytic processing has been reported for other proteins that are linked to the plasma membrane by a glycosyl...

  8. Risk Factors Associated with Serum Levels of the Inflammatory Biomarker Soluble Urokinase Plasminogen Activator Receptor in a General Population

    DEFF Research Database (Denmark)

    Haupt, Thomas H; Kallemose, Thomas; Ladelund, Steen;

    2014-01-01

    The soluble urokinase plasminogen activator receptor (suPAR) is a biomarker of mortality risk in various patient populations. However, little is known about the implications of lifestyle for suPAR levels in the general population. Lifestyle, demographic, and cardiovascular disease (CVD) risk factor.......001). An unhealthy diet and alcohol abstinence in men were also associated with higher suPAR levels. Physical activity in leisure time had a modest impact on suPAR levels in univariate analysis, but not in the final adjusted model. In conclusion, smoking and morbid obesity were strongly associated with higher serum...

  9. Serum soluble urokinase-type plasminogen activator receptor levels in male patients with acute exacerbation of schizophrenia.

    Science.gov (United States)

    Genc, Abdullah; Kalelioglu, Tevfik; Karamustafalioglu, Nesrin; Tasdemir, Akif; Genc, Esra Sena; Akkus, Mustafa; Emul, Murat

    2016-02-28

    Inflammatory abnormalities have been shown in the pathogenesis of schizophrenia. Soluble urokinase-type plasminogen activator receptor (suPAR) is a protein that is measurable in the circulating blood and reflects the inflammation in the body. We aimed to investigate serum suPAR levels in patients with schizophrenia who were in acute state and to compare with healthy controls. Forty five patients and 43 healthy controls were included in the study. We found no significant difference in suPAR levels between patients and controls, suggesting that suPAR as an inflammatory marker does not have a role in the inflammatory process of acute schizophrenia.

  10. Urokinase plasminogen activator and plasminogen activator inhibitor type-1 in nonsmall-cell lung cancer: relation to prognosis and angiogenesis

    DEFF Research Database (Denmark)

    Offersen, Birgitte Vrou; Pfeiffer, Per; Andreasen, Peter;

    2007-01-01

    BACKGROUND: Urokinase plasminogen activator (uPA) and plasminogen activator inhibitor type-1 (PAI-1) have previously been suggested as prognostic markers in nonsmall-cell lung carcinomas (NSCLC). We investigate whether uPA and PAI-1 are prognostic markers in NSCLC and whether they are related...... identified as non-angiogenic we found significantly lower contents of both uPA and PAI-1 as compared to angiogenic tumours, thus we hypothesize that uPA and PAI-1 stimulate angiogenesis in NSCLC. Udgivelsesdato: 2007-Apr...

  11. The urokinase receptor (uPAR facilitates clearance of Borrelia burgdorferi.

    Directory of Open Access Journals (Sweden)

    Joppe W R Hovius

    2009-05-01

    Full Text Available The causative agent of Lyme borreliosis, the spirochete Borrelia burgdorferi, has been shown to induce expression of the urokinase receptor (uPAR; however, the role of uPAR in the immune response against Borrelia has never been investigated. uPAR not only acts as a proteinase receptor, but can also, dependently or independently of ligation to uPA, directly affect leukocyte function. We here demonstrate that uPAR is upregulated on murine and human leukocytes upon exposure to B. burgdorferi both in vitro as well as in vivo. Notably, B. burgdorferi-inoculated C57BL/6 uPAR knock-out mice harbored significantly higher Borrelia numbers compared to WT controls. This was associated with impaired phagocytotic capacity of B. burgdorferi by uPAR knock-out leukocytes in vitro. B. burgdorferi numbers in vivo, and phagocytotic capacity in vitro, were unaltered in uPA, tPA (low fibrinolytic activity and PAI-1 (high fibrinolytic activity knock-out mice compared to WT controls. Strikingly, in uPAR knock-out mice partially backcrossed to a B. burgdorferi susceptible C3H/HeN background, higher B. burgdorferi numbers were associated with more severe carditis and increased local TLR2 and IL-1beta mRNA expression. In conclusion, in B. burgdorferi infection, uPAR is required for phagocytosis and adequate eradication of the spirochete from the heart by a mechanism that is independent of binding of uPAR to uPA or its role in the fibrinolytic system.

  12. Uncharged isocoumarin-based inhibitors of urokinase-type plasminogen activator

    Directory of Open Access Journals (Sweden)

    Deck Lorraine M

    2006-02-01

    Full Text Available Abstract Background Urokinase-type plasminogen activator (uPA plays a major role in extracellular proteolytic events associated with tumor cell growth, migration and angiogenesis. Consequently, uPA is an attractive target for the development of small molecule active site inhibitors. Most of the recent drug development programs aimed at nonpeptidic inhibitors targeted at uPA have focused on arginino mimetics containing amidine or guanidine functional groups attached to aromatic or heterocyclic scaffolds. There is a general problem of limited bioavailability of these charged inhibitors. In the present study, uPA inhibitors were designed on an isocoumarin scaffold containing uncharged substituents. Results 4-Chloro-3-alkoxyisocoumarins were synthesized in which the 3-alkoxy group contained a terminal bromine; these were compared with similar inhibitors that contained a charged terminal functional group. Additional variations included functional groups attached to the seven position of the isocoumarin scaffold. N- [3-(3-Bromopropoxy-4-chloro-1-oxo-1H-isochromen-7-yl]benzamide was identified as an uncharged lead inhibitor of uPA, Ki = 0.034 μM. Molecular modeling of human uPA with these uncharged inhibitors suggests that the bromine occupies the same position as positively charged arginino mimetic groups. Conclusion This study demonstrates that potent uncharged inhibitors of uPA can be developed based upon the isocoumarin scaffold. A tethered bromine in the three position and an aromatic group in the seven position are important contributors to binding. Although the aim was to develop compounds that act as mechanism-based inactivators, these inhibitors are competitive reversible inhibitors.

  13. Chemotactic effect of urokinase-type plasminogen activator on mouse spermatozoa in vitro

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The aim of this study is to investigate the chemotactic effect of urokinase-type plasminogen activator (uPA)on mouse spermatozoa.Capillary assays were applied to study the chemotactic activity of ascending and descending gradients of uPA.Firstly,the chemotactic effect of an ascending gradient of uPA on mouse spermatozoa was observed by counting the number of spermatozoa that migrated into the capillary after incubation with uPA for 5,10,20,and 30 min,respectively,compared with that after incubation with F10.Twenty minutes was a suitable incubation time to obtain a plateau of sperm accumulation.Meanwhile,to confirm the specific effect of uPA on mouse sperm chemotaxis,uPA inhibitor (PAI-1)and anti-uPAR rabbit IgG were added to the test solution containing 20 U/mL uPA,respectively.To exclude the possibility that PAI-1 and anti-uPAR rabbit IgG may affect sperm accumulation nonspecifically,PAIl and anti-uPAR rabbit IgG were added to F10,respectively.It was found that the chemotactic effect of uPA was neutralized completely by PAI-1 and anti-uPAR rabbit IgG.PAI-1 and anti-uPAR rabbit IgG had no neutralizing effect on the sperm chemotactic effect.Lastly,the sperm chemotaxis response to a descending gradient of uPA was also observed.Taken together,the results suggest that uPA can induce sperm chemotaxis in vitro by binding to its receptor on the sperm membrane and may act as a chemoattractant in precontacting sperm-egg communication thereby increasing the chance encounter of spermatozoa and eggs.

  14. Hypoxia stimulates urokinase receptor expression through a heme protein-dependent pathway.

    Science.gov (United States)

    Graham, C H; Fitzpatrick, T E; McCrae, K R

    1998-05-01

    Hypoxia underlies a number of biologic processes in which cellular migration and invasion occur. Because earlier studies have shown that the receptor for urokinase-type plasminogen activator (uPAR) may facilitate such events, we studied the effect of hypoxia on the expression of uPAR by first trimester human trophoblasts (HTR-8/SVneo) and human umbilical vein endothelial cells (HUVEC). Compared with control cells cultured under standard conditions (20% O2), HTR-8/SVneo cells and HUVEC cultured in 1% O2 expressed more uPAR, as determined by flow cytometric and [125I]-prourokinase ligand binding analyses. Increased uPAR expression paralleled increases in uPAR mRNA. The involvement of a heme protein in the hypoxia-induced expression of uPAR was suggested by the observations that culture of cells with cobalt chloride, or sodium 4, 5-dihydroxybenzene-1,3-disulfonate (Tiron), an iron-chelating agent, also stimulated uPAR expression, and that the hypoxia-induced uPAR expression was inhibited by adding carbon monoxide to the hypoxic atmosphere. Culture of HTR-8/SVneo cells with vascular endothelial growth factor (VEGF) did not increase uPAR mRNA levels, suggesting that the hypoxia-mediated effect on uPAR expression by these cells did not occur through a VEGF-dependent mechanism. The functional importance of these findings is suggested by the fact that HTR-8/SVneo cells cultured under hypoxia displayed higher levels of cell surface plasminogen activator activity and greater invasion through a reconstituted basement membrane. These results suggest that hypoxia may promote cellular invasion by stimulating the expression of uPAR through a heme protein-dependent pathway. PMID:9558386

  15. Safety and Efficacy of Low Dosage of Urokinase for Catheter-directed Thrombolysis of Deep Venous Thrombosis

    Institute of Scientific and Technical Information of China (English)

    Xiao-Long Du; Ling-Shang Kong; Qing-You Meng; Aimin Qian; Wen-Dong Li; Hong Chen; Xiao-Qiang Li

    2015-01-01

    Background:Catheter-directed thrombolysis (CDT) has been a mainstay in treating deep venous thrombosis (DVT).However,the optimal dosage of a thrombolytic agent is still controversial.The goal of this study was to evaluate the safety and efficacy of low dosage urokinase with CDT for DVT.Methods:A retrospective analysis was performed using data from a total of 427 patients with DVT treated with CDT in our single center between July 2009 and December 2012.Early efficacy of thrombolysis was assessed with a thrombus score based on daily venography.The therapeutic safety was evaluated by adverse events.A venography or duplex ultrasound was performed to assess the outcome at 6 months,1 year and 2 years postoperatively.Results:The mean total dose of 3.34 (standard deviation [SD] 1.38) million units of urokinase was administered during a mean of 5.18 (SD 2.28) days.Prior to discharge,Grade Ⅲ (complete lysis) was achieved in 154 (36%) patients;Grade Ⅱ (50-99% lysis) in 222 (52%);and Grade Ⅰ (50% lysis) in 51 (12%).The major complications included one intracranial hemorrhage,one hematochezia,five gross hematuria,and one pulmonary embolism.Moreover,no death occurred in the study.Conclusions:Treatment of low-dose catheter-directed thrombosis is an efficacious and safe therapeutic approach in patients with DVT offering good long-term outcomes and minimal complications.

  16. Prognostic value of plasma soluble urokinase plasminogen activator receptor (suPAR) in Danish patients with recurrent epithelial ovarian cancer (REOC)

    DEFF Research Database (Denmark)

    Begum, Farah Diba; Høgdall, Estrid V S; Riisbo, Rikke;

    2006-01-01

    The level of the soluble urokinase plasminogen activator receptor (suPAR) is elevated in tumour tissue from several types of cancer. This is the first study aiming to predict the prognosis for survival by the use of a pre-chemotherapeutic plasma suPAR value in 71 patients with recurrent epithelial...

  17. Mimicry of the regulatory role of urokinase in lamellipodia formation by introduction of a non-native interdomain disulfide bond in its receptor

    DEFF Research Database (Denmark)

    Gårdsvoll, Henrik; Kjærgaard, Magnus; Jacobsen, Benedikte;

    2011-01-01

    The high-affinity interaction between the urokinase-type plasminogen activator (uPA) and its glycolipid-anchored receptor (uPAR) plays a regulatory role for both extravascular fibrinolysis and uPAR-mediated adhesion and migration on vitronectin-coated surfaces. We have recently proposed that the ...

  18. High plasma levels of intact and cleaved soluble urokinase receptor reflect immune activation and are independent predictors of mortality in HIV-1-infected patients

    DEFF Research Database (Denmark)

    Ostrowski, Sisse Rye; Piironen, Timo; Høyer-Hansen, Gunilla;

    2005-01-01

    BACKGROUND: High blood levels of soluble urokinase receptor (suPAR) measured by enzyme-linked immunoassay (ELISA) (bulk measurement of 3-domain and 2-domain suPAR [suPAR(I-III), suPAR(II-III)], and suPAR(I-III) ligand complexes) strongly predict mortality in HIV-1-infected patients. This study in...

  19. High plasma levels of intact and cleaved soluble urokinase receptor reflect immune activation and are independent predictors of mortality in HIV-1-infected patients

    DEFF Research Database (Denmark)

    Ostrowski, Sisse Rye; Piironen, Timo; Høyer-Hansen, Gunilla;

    2005-01-01

    High blood levels of soluble urokinase receptor (suPAR) measured by enzyme-linked immunoassay (ELISA) (bulk measurement of 3-domain and 2-domain suPAR [suPAR(I-III), suPAR(II-III)], and suPAR(I-III) ligand complexes) strongly predict mortality in HIV-1-infected patients. This study investigated p...

  20. Soluble Urokinase Plasminogen Activator Receptor Level Is an Independent Predictor of the Presence and Severity of Coronary Artery Disease and of Future Adverse Events

    DEFF Research Database (Denmark)

    Eapen, Danny J; Manocha, Pankaj; Ghasemzedah, Nima;

    2014-01-01

    INTRODUCTION: Soluble urokinase plasminogen activator receptor (suPAR) is an emerging inflammatory and immune biomarker. Whether suPAR level predicts the presence and the severity of coronary artery disease (CAD), and of incident death and myocardial infarction (MI) in subjects with suspected CAD...

  1. A 55,000-60,000 Mr receptor protein for urokinase-type plasminogen activator. Identification in human tumor cell lines and partial purification

    DEFF Research Database (Denmark)

    Nielsen, L S; Kellerman, G M; Behrendt, N;

    1988-01-01

    The iodinated Mr approximately equal to 15,000 amino-terminal fragment (ATF) of the urokinase-type plasminogen activator (u-PA) molecule bound specifically to the cell surface of all of seven cultured human tumor cell lines studied. Cross-linking of iodinated ATF to the cell surface using a bifun...

  2. Urokinase receptor mRNA level and gene transcription are strongly and rapidly increased by phorbol myristate acetate in human monocyte-like U937 cells

    DEFF Research Database (Denmark)

    Lund, L R; Rønne, E; Roldan, A L;

    1991-01-01

    We have studied the effect of the tumor promotor phorbol myristate acetate (PMA) on the level of mRNA for the receptor for urokinase-type plasminogen activator (u-PAR) in the human monocyte-like cell line U937. PMA causes an early increase in the u-PAR mRNA level which reaches a maximal 50-fold e...

  3. The immune marker soluble urokinase plasminogen activator receptor is associated with new-onset diabetes in non-smoking women and men

    DEFF Research Database (Denmark)

    Haugaard, S B; Andersen, O; Hansen, T W;

    2012-01-01

    Aim: To explore the putative association of new-onset diabetes and the soluble urokinase plasminogen activator receptor (suPAR), which is a new and stable plasma marker of immune function and low-grade inflammation. This association has been previously suggested by using the less sensitive Intern...

  4. The “Lipid Accumulation Product” Is Associated with 2-Hour Postload Glucose Outcomes in Overweight/Obese Subjects with Nondiabetic Fasting Glucose

    Science.gov (United States)

    Malavazos, Alexis Elias; Cereda, Emanuele; Ermetici, Federica; Caccialanza, Riccardo; Briganti, Silvia; Rondanelli, Mariangela; Morricone, Lelio

    2015-01-01

    “Lipid accumulation product” (LAP) is a continuous variable based on waist circumference and triglyceride concentration previously associated with insulin resistance. We investigated the accuracy of LAP in identifying oral glucose tolerance test (OGTT) abnormalities and compared it to the homeostasis model assessment of insulin resistance (HOMA-IR) in a population of overweight/obese outpatients presenting with nondiabetic fasting glucose. We studied 381 (male: 23%) adult (age: 18–70 years) overweight/obese Caucasians (body mass index: 36.9 ± 5.4 Kg/m2) having fasting plasma glucose < 7.0 mmol/L. OGTT was used to diagnose unknown glucose tolerance abnormalities: impaired glucose tolerance (IGT) and type-2 diabetes mellitus (T2-DM). According to OGTT 92, subjects had an IGT and 33 were diagnosed T2-DM. Logistic regression analysis detected a significant association for both LAP and HOMA-IR with single (IGT and T2-DM) and composite (IGT + T2-DM) abnormal glucose tolerance conditions. However, while the association with diabetes was similar between LAP and HOMA-IR, the relationship with IGT and composite outcomes by models including LAP was significantly superior to those including HOMA-IR (P = 0.006 and P = 0.007, resp.). LAP seems to be an accurate index, performing better than HOMA-IR, for identifying 2-hour postload OGTT outcomes in overweight/obese patients with nondiabetic fasting glucose. PMID:25792981

  5. The “Lipid Accumulation Product” Is Associated with 2-Hour Postload Glucose Outcomes in Overweight/Obese Subjects with Nondiabetic Fasting Glucose

    Directory of Open Access Journals (Sweden)

    Alexis Elias Malavazos

    2015-01-01

    Full Text Available “Lipid accumulation product” (LAP is a continuous variable based on waist circumference and triglyceride concentration previously associated with insulin resistance. We investigated the accuracy of LAP in identifying oral glucose tolerance test (OGTT abnormalities and compared it to the homeostasis model assessment of insulin resistance (HOMA-IR in a population of overweight/obese outpatients presenting with nondiabetic fasting glucose. We studied 381 (male: 23% adult (age: 18–70 years overweight/obese Caucasians (body mass index: 36.9 ± 5.4 Kg/m2 having fasting plasma glucose < 7.0 mmol/L. OGTT was used to diagnose unknown glucose tolerance abnormalities: impaired glucose tolerance (IGT and type-2 diabetes mellitus (T2-DM. According to OGTT 92, subjects had an IGT and 33 were diagnosed T2-DM. Logistic regression analysis detected a significant association for both LAP and HOMA-IR with single (IGT and T2-DM and composite (IGT + T2-DM abnormal glucose tolerance conditions. However, while the association with diabetes was similar between LAP and HOMA-IR, the relationship with IGT and composite outcomes by models including LAP was significantly superior to those including HOMA-IR (P=0.006 and P=0.007, resp.. LAP seems to be an accurate index, performing better than HOMA-IR, for identifying 2-hour postload OGTT outcomes in overweight/obese patients with nondiabetic fasting glucose.

  6. Combined use of serum adiponectin and tumor necrosis factor-alpha receptor 2 levels was comparable to 2-hour post-load glucose in diabetes prediction.

    Directory of Open Access Journals (Sweden)

    Yu-Cho Woo

    Full Text Available BACKGROUND: Adipose tissue inflammation and dysregulated adipokine secretion are implicated in obesity-related insulin resistance and type 2 diabetes. We evaluated the use of serum adiponectin, an anti-inflammatory adipokine, and several proinflammatory adipokines, as biomarkers of diabetes risk and whether they add to traditional risk factors in diabetes prediction. METHODS: We studied 1300 non-diabetic subjects from the prospective Hong Kong Cardiovascular Risk Factor Prevalence Study (CRISPS. Serum adiponectin, tumor necrosis factor-alpha receptor 2 (TNF-α R2, interleukin-6 (IL-6, adipocyte-fatty acid binding protein (A-FABP and high-sensitivity C-reactive protein (hsCRP were measured in baseline samples. RESULTS: Seventy-six participants developed diabetes over 5.3 years (median. All five biomarkers significantly improved the log-likelihood of diabetes in a clinical diabetes prediction (CDP model including age, sex, family history of diabetes, smoking, physical activity, hypertension, waist circumference, fasting glucose and dyslipidaemia. In ROC curve analysis, "adiponectin + TNF-α R2" improved the area under ROC curve (AUC of the CDP model from 0.802 to 0.830 (P = 0.03, rendering its performance comparable to the "CDP + 2-hour post-OGTT glucose" model (AUC = 0.852, P = 0.30. A biomarker risk score, derived from the number of biomarkers predictive of diabetes (low adiponectin, high TNF-α R2, had similar performance when added to the CDP model (AUC = 0.829 [95% CI: 0.808-0.849]. CONCLUSIONS: The combined use of serum adiponectin and TNF-α R2 as biomarkers provided added value over traditional risk factors for diabetes prediction in Chinese and could be considered as an alternative to the OGTT.

  7. Structure-based engineering of species selectivity in the interaction between urokinase and its receptor: implication for preclinical cancer therapy

    DEFF Research Database (Denmark)

    Lin, Lin; Gårdsvoll, Henrik; Huai, Qing;

    2010-01-01

    The high affinity interaction between the urokinase-type plasminogen activator (uPA) and its glycolipid-anchored receptor (uPAR) is decisive for cell surface-associated plasminogen activation. Because plasmin activity controls fibrinolysis in a variety of pathological conditions, including cancer...... and wound healing, several intervention studies have focused on targeting the uPA.uPAR interaction in vivo. Evaluations of such studies in xenotransplanted tumor models are, however, complicated by the pronounced species selectivity in this interaction. We now report the molecular basis underlying...... the structural basis required for a successful rational design of the species selectivity in the uPA.uPAR interaction, which is highly relevant for functional studies in mouse models, but it also suggests the possible development of general inhibitors that will target the uPA.uPAR interaction across species...

  8. The plasma level of soluble urokinase receptor is elevated in patients with Streptococcus pneumoniae bacteraemia and predicts mortality

    DEFF Research Database (Denmark)

    Wittenhagen, P; Kronborg, G; Weis, N;

    2004-01-01

    This multicentre prospective study was conducted to investigate whether the level of the soluble form of urokinase-type plasminogen activator receptor (suPAR) is elevated during pneumococcal bacteraemia and is of predictive value in the early stage of the disease. Plasma levels of suPAR were...... increased significantly (median 5.5; range 2.4-21.0 ng/mL) in 141 patients with pneumococcal bacteraemia, compared to 31 healthy controls (median 2.6, range 1.5-4.0 ng/mL, p 0.001). Furthermore, suPAR levels were elevated significantly in patients who died from the infection (n = 24) compared to survivors...... (n = 117; p mortality (p

  9. The complex between urokinase (uPA) and its type-1 inhibitor (PAI-1) in pulmonary adenocarcinoma: Relation to prognosis

    DEFF Research Database (Denmark)

    Pappot, Helle; Pedersen, Anders N; Brünner, Nils;

    2006-01-01

    In a lung cancer population comprising tumor tissue from 99 pulmonary adenocarcinoma patients, the relationship between tumor tissue level of the complex formed of urokinase (uPA) and its type-1 inhibitor (PAI-1) and survival was studied. The study included patient material previously investigated....... The amounts of uPA-PAI-1 complex measured in pulmonary adenocarcinoma tissue were within the same range as previously reported in breast cancer tissue (0.11-5.74 ng/mg protein). uPA and PAI-1 levels were weakly correlated to the uPA-PAI-1 complex, r = 0.52 and r = 0.47, respectively, and no relation was found...... these interactions and the clinical importance of the tissue levels of uPA, PAI-1 and uPA-PAI-1 complex, the results suggest further exploratory studies of the components in pulmonary adenocarcinomas and other cancers....

  10. The complex between urokinase (uPA) and its type-1 inhibitor (PAI-1) in pulmonary adenocarcinoma

    DEFF Research Database (Denmark)

    Pappot, Helle; Pedersen, Anders N.; Brünner, Nils;

    2006-01-01

    In a lung cancer population comprising tumor tissue from 99 pulmonary adenocarcinoma patients, the relationship between tumor tissue level of the complex formed of urokinase (uPA) and its type-1 inhibitor (PAI-1) and survival was studied. The study included patient material previously investigated....... The amounts of uPA-PAI-1 complex measured in pulmonary adenocarcinoma tissue were within the same range as previously reported in breast cancer tissue (0.11-5.74 ng/mg protein). uPA and PAI-1 levels were weakly correlated to the uPA-PAI-1 complex, r = 0.52 and r = 0.47, respectively, and no relation was found...... these interactions and the clinical importance of the tissue levels of uPA, PAI-1 and uPA-PAI-1 complex, the results suggest further exploratory studies of the components in pulmonary adenocarcinomas and other cancers....

  11. The plasma level of soluble urokinase receptor is elevated in patients with Streptococcus pneumoniae bacteraemia and predicts mortality

    DEFF Research Database (Denmark)

    Wittenhagen, p; Kronborg, Gitte; Nielsen, H;

    2004-01-01

    This multicentre prospective study was conducted to investigate whether the level of the soluble form of urokinase-type plasminogen activator receptor (suPAR) is elevated during pneumococcal bacteraemia and is of predictive value in the early stage of the disease. Plasma levels of suPAR were...... (n = 117; p cerebral symptoms and high serum concentrations of protein YKL-40 and suPAR were associated significantly with mortality (p ....05). In multivariate analysis, only suPAR remained a significant predictor of death (mortality rate of 13 for suPAR levels of > 10 ng/mL; 95% CI: 1.1-158). The increase in suPAR levels may reflect increased expression by vascular or inflammatory cells in the setting of pneumococcal sepsis. This plasma protein may...

  12. Soluble urokinase plasminogen activator receptor levels are elevated and associated with complications in patients with type 1 diabetes

    DEFF Research Database (Denmark)

    Theilade, S; Lyngbaek, S; Hansen, T W;

    2015-01-01

    OBJECTIVES: Soluble urokinase plasminogen activator receptor (suPAR) is a marker of inflammation and endothelial dysfunction. We investigated the associations between suPAR and diabetes, including diabetes duration and complications, in patients with type 1 diabetes. DESIGN, SETTING AND SUBJECTS...... diabetes and is associated with diabetes duration and complications independent of other risk factors. suPAR is a potential novel risk marker for the management of diabetes.......: From 2009 to 2011, 667 patients with type 1 diabetes and 51 nondiabetic control subjects were included in a cross-sectional study at Steno Diabetes Center, Gentofte, Denmark. suPAR levels were measured with an enzyme-linked immunosorbent assay. MAIN OUTCOME MEASURES: The investigated diabetic...

  13. Soluble urokinase receptor levels in plasma during 5 years of highly active antiretroviral therapy in HIV-1-infected patients

    DEFF Research Database (Denmark)

    Ostrowski, Sisse R; Katzenstein, Terese L; Piironen, Timo;

    2004-01-01

    High blood levels of the soluble urokinase receptor (suPAR) strongly predict increased mortality in human immunodeficiency virus-1 (HIV-1)-infected patients. This study investigated the plasma concentration of suPAR in 29 treatment-naive HIV-1-infected patients during 5 years treatment with highly...... active antiretroviral therapy (HAART). Plasma suPAR decreased after introducing HAART, most pronounced during the first treatment year. The change in plasma suPAR was independent of changes in viral replication and CD4+ cells but it was strongly correlated with plasma levels of the soluble TNF receptor...... II. Compared with healthy individuals, plasma suPAR and sTN-FrII was increased in untreated patients. After initiating HAART, plasma sTNFrII remained increased whereas plasma suPAR decreased to a level comparable with healthy individuals. The present data indicate that the circulating suPAR level is...

  14. Effect of low-molecular-weight heparin and urokinase on pulmonary arteries involved in pulmonary embolism

    Institute of Scientific and Technical Information of China (English)

    WU Jun-ping; SUN Xin; WU Qi; DU Zhong-zhen; LI Li; WU Qian; SUN Hong-fen

    2013-01-01

    Background Pulmonary embolism (PE) is a common and often fatal disease.Early after pulmonary thromboembolism,inflammation and associated intimal hyperplasia occur within the pulmonary arteries,similar to what is observed with chronic thromboembolic pulmonary hypertension.This study tested the hypothesis that thrombolytic and anticoagulant agents would have anti-inflammatory effects or inhibit intimal hyperplasia of involved pulmonary arteries.Methods Seventy-two male New Zealand white rabbits were randomly divided into two groups (54 rabbits in the PE group and 18 in the sham group).Experimental PE was induced in 54 rabbits by femoral vein injection of autologous blood clots and confirmed with pulmonary angiography,and other 18 rabbits underwent sham operations.Fifty-four rabbits in the PE group were randomly divided into three groups:a control group (treated with normal saline),a low-molecularweight heparin (LMWH) group (treated with LMWH),and a urokinase (UK) group (treated with UK).Arterial blood gas was analyzed at 2,7,and 28 days (n=6 per time point by random group division),then lung tissues were removed and were analyzed for pro-inflammatory cytokines and chemokines,and were stained for intimal hyperplasia.Results The overall survival of rabbits undergoing PE was 100%.PE distribution detected on digital signal angiography (DSA) and histopathology was shown in 67% of rabbits (36/54) in the bilateral low lobar pulmonary arteries (PAs).The results showed that alveolar-arterial partial pressure of oxygen (PO2) difference (PA-aO2) significantly increased and PO2 decreased in the control group compared with the sham group.Compared with controls,the UK group had a decreased level of PA-aO2 on day 2 (P <0.05),however,there was no significant difference in the LMWH group.Compared with controls,the LMWH group had a decreased level of monocyte chemoattractant protein-1 (MCP-1) in affected tissue and serum samples on days 7 and 28 (P <0.05),and the UK group had

  15. Preclinical evaluation of a urokinase plasminogen activator receptor-targeted nanoprobe in rhesus monkeys

    Directory of Open Access Journals (Sweden)

    Chen Y

    2015-10-01

    Full Text Available Yushu Chen,1 Li Gong,2 Ning Gao,3 Jichun Liao,1 Jiayu Sun,1 Yuqing Wang,1 Lei Wang,1 Pengjin Zhu,1 Qing Fan,1 Yongqiang Andrew Wang,4 Wen Zeng,2 Hui Mao,3 Lily Yang,5 Fabao Gao11Molecular Imaging Center, Department of Radiology, West China Hospital, Sichuan University, Chengdu, 2Sichuan Primed Bio-Tech Group Co, Ltd, Chengdu, People’s Republic of China; 3Department of Radiology and Imaging Sciences, Emory University School of Medicine, Atlanta, GA, 4Ocean NanoTech, LLC, San Diego, CA, 5Department of Surgery, Emory University School of Medicine, Atlanta, GA, USAPurpose: To translate a recombinant peptide containing the amino-terminal fragment (ATF of urokinase plasminogen activator receptor-targeted magnetic iron oxide (IO nanoparticles (uPAR-targeted human ATF-IONPs into clinical applications, we conducted a pilot study to evaluate the toxicity and pharmacokinetics of this nanoparticle in normal rhesus monkeys.Methods: We assessed the changes in the following: magnetic resonance imaging (MRI signals from pretreatment stage to 14 days posttreatment, serum iron concentrations from 5 minutes posttreatment to 12 weeks posttreatment, routine blood examination and serum chemistry analysis results from pretreatment stage to 12 weeks after administration, and results of staining of the liver with Perls’ Prussian Blue and hematoxylin–eosin at 24 hours and 3 months posttreatment in two rhesus monkeys following an intravenous administration of the targeted nanoparticles either with a polyethylene glycol (ATF-PEG-IONP or without a PEG (ATF-IONP coating.Results: The levels of alkaline phosphatase, alanine transaminase, and direct bilirubin in the two monkeys increased immediately after the administration of the IONPs but returned to normal within 20 days and stayed within the normal reference range 3 months after the injection. The creatinine levels of the two monkeys stayed within the normal range during the study. In addition, red blood cells

  16. Construction, Expression, and Characterization of a Recombinant Annexin B1-Low Molecular Weight Urokinase Chimera in Escherichia coli

    Institute of Scientific and Technical Information of China (English)

    Hong-Li YAN; Wei-Ting WANG; Yan HE; Zhuan-You ZHAO; Yuan-Jian GAO; Yi ZHANG; Shu-Han SUN

    2004-01-01

    To produce a thrombi-targeting plasminogen activator,low molecular weight single-chain urokinase gene(scuPA32k)was spliced with the full-length cDNA of annexin B 1 gene(anxB1)by overlap extension method.The fused gene anxBlscuPA was ligated into pET28a vector,transformed into E.coli BL21-RIL,and then induced to express under the control of T7 promoter.The AnxB 1ScuPA protein expressed amounted to 22% of the total bacterial proteins.The product was refolded,and then purified by using DEAE Sepharose fast flow ion-exchange column and Superdex S-200 gel-filtration column.HPLC analysis revealed that the final purity is about 95%.The specific activity ofAnxB 1ScuPA,measured as amidolytic activity,reached 100,000 IU/mg.It had a similar S2444 catalytic efficiency(kcat/Km)to ScuPA32k,and also showed high activated-platelet membrane-binding activity and anticoagulant activity,indicating that the chimera fully retained the components of enzymatic and membrane-binding activities of the parent molecules.In vivo test revealed that,the dogs administered with AnxB 1ScuPA had less reperfusion time,higher reperfusion ratio,and less bleeding effects than those with urokinase.These findings indicated that AnxB 1ScuPAmight have advantages over current available thrombolytic agents.

  17. Influential factors of clinical outcome of local intra-arterial thrombolysis using urokinase in patients with hyperacute ischemic stroke

    International Nuclear Information System (INIS)

    To evaluate the clinical outcome and other relevant factors in cases where local intra-arterial thrombolysis (LIT) is used for the treatment of hyperacute ischemic stroke. Forty-eight hyperacute ischemic stroke patients were treated by LIT, using urokinase, within six hours of ictus, and for evaluation of their neurological status, the national institutes of health stroke scale (NIHSS) score was used. Angiography recanalization was classified according to Mori recanalization grades. Three months after LIT, the outcome was assessed by clinical examination using the modified rankin scale (good outcome: RS=0-3; poor outcome: RS=4-6). In all patients, the findings of pre- and post- LIT CT, and angiography, as well as neurological status and hemorrhagic complications, were also analysed. Thirty-three patients had occlusions of the middle cerebral artery (MCA), and 15, of the internal carotid artery (ICA). The NIHSS score averaged 16.9 at the onset of therapy and 13.5 at 24 hours later. Successful recanalization (Mori grade 3,4) was achieved in 28 (58.3%) of 48 patients, but in 20 (41.7%) the attempt failed. Twenty-two (45.8%) of the 48 patients had a good outcome, but in (54.2%) the outcome was poor. Thirteen (40.6%) of 32 patients with MCA occlusions and 13 (81.2%) of 16 with ICA occlusions had a poor outcome. Eight patients (16.7%) died. Overall, hemorrhages occured in 20 (41.7%) of 48 patients, with symptomatic hemorrhage in ten. Five (50%) of these ten died. LIT using urokinase for hyperacute ischemic stroke is feasible; patients with MCA occlusions had better outcomes than those with ICA occlusions. Hemorrhagic complications of LIT were frequent, and in cases of symptomatic hemorrhage a fatal outcome may be expected

  18. Influential factors of clinical outcome of local intra-arterial thrombolysis using urokinase in patients with hyperacute ischemic stroke

    Energy Technology Data Exchange (ETDEWEB)

    Song, Jae Min; Yoon, Woong; Kim, Jae Kyu; Seo, Jeong Jin; Heo, Sook Hee; Park, Jin Gyoon; Jeong, Yoon Yeon; Kang, Heoung Keun [Chonam University Hospital, Kwangju (Korea, Republic of)

    2002-10-01

    To evaluate the clinical outcome and other relevant factors in cases where local intra-arterial thrombolysis (LIT) is used for the treatment of hyperacute ischemic stroke. Forty-eight hyperacute ischemic stroke patients were treated by LIT, using urokinase, within six hours of ictus, and for evaluation of their neurological status, the national institutes of health stroke scale (NIHSS) score was used. Angiography recanalization was classified according to Mori recanalization grades. Three months after LIT, the outcome was assessed by clinical examination using the modified rankin scale (good outcome: RS=0-3; poor outcome: RS=4-6). In all patients, the findings of pre- and post- LIT CT, and angiography, as well as neurological status and hemorrhagic complications, were also analysed. Thirty-three patients had occlusions of the middle cerebral artery (MCA), and 15, of the internal carotid artery (ICA). The NIHSS score averaged 16.9 at the onset of therapy and 13.5 at 24 hours later. Successful recanalization (Mori grade 3,4) was achieved in 28 (58.3%) of 48 patients, but in 20 (41.7%) the attempt failed. Twenty-two (45.8%) of the 48 patients had a good outcome, but in (54.2%) the outcome was poor. Thirteen (40.6%) of 32 patients with MCA occlusions and 13 (81.2%) of 16 with ICA occlusions had a poor outcome. Eight patients (16.7%) died. Overall, hemorrhages occured in 20 (41.7%) of 48 patients, with symptomatic hemorrhage in ten. Five (50%) of these ten died. LIT using urokinase for hyperacute ischemic stroke is feasible; patients with MCA occlusions had better outcomes than those with ICA occlusions. Hemorrhagic complications of LIT were frequent, and in cases of symptomatic hemorrhage a fatal outcome may be expected.

  19. Political Regimes and Economic Growth

    Directory of Open Access Journals (Sweden)

    Carlos Pinho

    2009-12-01

    Full Text Available Are political regimes drivers of economic growth? While political institutions are influenced by economic development, they are in turn a key determinant of the development process. This study builds in the Neoclassical Growth theory to identify the influence of political regimes on economic development through a panel data sample of 170 countries from 1960 to 2000. Results suggest that once fixed effects are considered, the positive relationship between income per capita and political regimes measured by different democracy variables disappears.

  20. Reduced release of intact and cleaved urokinase receptor in stimulated whole-blood cultures from human immunodeficiency virus-1-infected patients

    DEFF Research Database (Denmark)

    Ostrowski, S R; Piironen, T; Høyer-Hansen, G;

    2005-01-01

    The blood levels of the soluble forms of the urokinase receptor (suPAR) are increased in human immunodeficiency virus (HIV)-1-infected patients. This study investigated whether the release of urokinase-type plasminogen activator receptor (uPAR) in whole-blood cultures was affected by HIV infection...... controls, whereas the correlation was weaker to leucocytes and nonexisting to circulating suPAR levels in HIV patients. These findings demonstrate that HIV infection affects stimulated and spontaneous uPAR release in whole-blood cultures. Given that high blood levels of suPAR in HIV patients are linked to....... The release of different uPAR forms in whole-blood cultures incubated 24 h with or without phytohemagglutinin and lipopolysaccharide was analysed in 47 HIV patients and 19 controls. suPAR was measured by enzyme-linked immunosorbent assay (ELISA) (bulk-suPAR) and three different time...

  1. Mapping the topographic epitope landscape on the urokinase plasminogen activator receptor (uPAR) by surface plasmon resonance and X-ray crystallography

    DEFF Research Database (Denmark)

    Zhao, Baoyu; Gandhi, Sonu; Yuan, Cai;

    2015-01-01

    The urokinase-type plasminogen activator receptor (uPAR or CD87) is a glycolipid-anchored membrane protein often expressed in the microenvironment of invasive solid cancers and high levels are generally associated with poor patient prognosis (Kriegbaum et al., 2011 [1]). uPAR is organized as a dy...... of these mAbs by X-ray crystallography alone and in complex with uPAR [deposited in the PDB database as 4QTH and 4QTI, respectively]....

  2. Soluble urokinase-type plasminogen activator receptor levels in patients with burn injuries and inhalation trauma requiring mechanical ventilation: an observational cohort study

    OpenAIRE

    Backes, Yara; van der Sluijs, Koenraad F; Tuip de Boer, Anita M; Hofstra, Jorrit Jan; Vlaar, Alexander PJ; Determann, Rogier M; Knape, Paul; David P Mackie; Schultz, Marcus J.

    2011-01-01

    Introduction Soluble urokinase-type plasminogen activator receptor (suPAR) has been proposed as a biologic marker of fibrinolysis and inflammation. The aim of this study was to investigate the diagnostic and prognostic value of systemic and pulmonary levels of suPAR in burn patients with inhalation trauma who need mechanical ventilation. Methods suPAR was measured in plasma and nondirected lung-lavage fluid of mechanically ventilated burn patients with inhalation trauma. The samples were obta...

  3. Urokinase plasminogen activator (uPA and plasminogen activator inhibitor type-1 (PAI-1 in breast cancer - correlation with traditional prognostic factors

    Directory of Open Access Journals (Sweden)

    Lampelj Maja

    2015-12-01

    Full Text Available Background. Urokinase plasminogen activator (uPA and plasminogen activator inhibitor type-1 (PAI-1 play a key role in tumour invasion and metastasis. High levels of both proteolytic enzymes are associated with poor prognosis in breast cancer patients. The purpose of this study was to evaluate the correlation between traditional prognostic factors and uPA and PAI-1 expression in primary tumour of breast cancer patients.

  4. Phorbol ester induces the biosynthesis of glycosylated and nonglycosylated plasminogen activator inhibitor 2 in high excess over urokinase-type plasminogen activator in human U-937 lymphoma cells

    OpenAIRE

    1987-01-01

    The tumor-promoting phorbol ester PMA induces changes in the histiocytic human lymphoma cell line U-937 akin to cellular differentiation (Ralph, P., N. Williams, M. A. S. Moore, and P. B. Litcofsky, 1982, Cell. Immunol., 71:215-223) and concomitantly stimulates the biosynthesis of plasminogen activator inhibitor 2 (PAI 2) and of urokinase-type plasminogen activator (u-PA). PAI 2 is found in a nonglycosylated intracellular and a glycosylated secreted form. The former appears to be identical to...

  5. Unitary Housing Regimes in Transition

    DEFF Research Database (Denmark)

    Bengtsson, Bo; Jensen, Lotte

    2013-01-01

    to the Danish and Swedish housing regimes are analysed and the responses and outcomes in terms of policy change and/or institutional continuity (path dependence) are compared. Overall, the more decentralized Danish housing regime seems to have resisted pressures for change and retrenchment better so far than...

  6. 'Regime shopping' across (blurring) boundaries

    NARCIS (Netherlands)

    Houwerzijl, M.S.; Evju, Stein

    2014-01-01

    This book chapter identifies and explores the (blurring) boundaries between the legal regimes for labour mobility across the EU. In the context of - what is sometimes called - 'regime shopping' a close look is taken into the law on freedom of movement within the EU. Several categories of transnation

  7. Regime shifts in resource management

    NARCIS (Netherlands)

    de Zeeuw, A.J.

    2014-01-01

    Resource management has to take account of the possibility of tipping points and regime shifts in ecological systems that provide the resources. This article focuses on the typical model of regime shifts in the ecological literature and analyzes optimal management and common-property issues when tra

  8. The Choice of Monetary Regime

    DEFF Research Database (Denmark)

    Østrup, Finn

    , a comparisonbetween monetary regimes suggests that welfare is highest under nominalincome targeting where the nominal income target is determined to bring aboutprice stability.Keywords: Monetary regimes; fiscal policy; monetary non-neutrality.JEL classicification: E42, E61, E62.......The article examines how government spending is determined in a closedeconomy where the nominal wage is pre-set through contracts and the wage settershave perfect foresight regarding subsequent policy decisions. The monetaryregime affects government spending because: (i) with a pre-set nominal wage......, agiven change in government spending has different effects on employment andinflation under different monetary regimes, and (ii) the authorities' inclinationto expand government spending is affected by the inflation rate which dependson the monetary regime. If the costs related to inflation are high...

  9. ELISA for complexes between urokinase-type plasminogen activator and its receptor in lung cancer tissue extracts

    DEFF Research Database (Denmark)

    de Witte, H; Pappot, H; Brünner, N;

    1997-01-01

    A sandwich-type ELISA has been developed for the assessment of complexes between urokinase-type plasminogen activator (uPA) and its receptor (uPAR) in extracts of squamous cell lung carcinomas. The assay is based on a combination of rabbit polyclonal anti-uPA antibodies and a biotinylated mouse...... extraction of uPAR yields the highest amounts of uPA:uPAR complexes. Absorption of tumor extracts with anti-uPA or anti-uPAR MAbs results in a complete disappearance of the ELISA signal, demonstrating the specificity of the ELISA. The recovery of chemically cross-linked uPA:uPAR complexes added to tumor...... extracts varies between 80% and 105%. The intra- and inter-assay variation coefficients are 5.3% and 9.8%, respectively. Furthermore, a peptide antagonist for uPAR was employed to evaluate de novo uPA:uPAR complex formation during tumor tissue extraction and the immunoassay procedure. Our results strongly...

  10. Interventional recanalization of artificial arteriovenous fistula and graft for hemodialysis: angioplasty and pulsed-spray thrombolysis with Urokinase

    International Nuclear Information System (INIS)

    To evaluate the effectiveness of percutaneous transluminal angioplasty (PTA) and pul-sed-spray pharmaco-mechanical thrombolysis (PSPMT) using urokinase for the management of insufficient hemodialysis access. Between September 1996 and May 1998, 21 insufficient hemodialysis accesses were treated in 16 patients(3 artificial arteriovenous fistulae, AVF; and 13 arteriovenous graft, AVG). PTA and PSPMT were performed in 6 and 15 cases, respectively, and success and long-term patency rates were evaluated. The overall success rate of PTA and PSPMT for insufficient hemodialysis access was 76. 2%(16/21). The success rates of PTA and PSPMT were 83.3%(5/6) and 73.3%(11/15), respectively. The primary patency rates of PSPMT were 69±12.8% at 6 months and 38±18.6% at 12 months. One of the two initially successful PTAs had been patent for 7 months, and the second PTA was performed at that time due to venous stenosis. The other was patent for 15 months throughout the follow-up period. PTA and PSPMT are effective primary methods for the treatment of insufficient hemodialysis access;success and patency rates were high, and the procedures can be performed repeatedly.=20

  11. Prognostic significance of circulating intact and cleaved forms of urokinase plasminogen activator receptor in inoperable chemotherapy treated cholangiocarcinoma patients

    DEFF Research Database (Denmark)

    Grunnet, M; Christensen, I J; Lassen, U;

    2014-01-01

    determine if pre-treatment serum levels of uPAR forms and a decrease in levels during chemotherapy are predictive of survival in patients with inoperable cholangiocarcinoma. DESIGN AND METHODS: Patients with inoperable cholangiocarcinoma were consecutively included in the training set (n=108). A test set......PAR(I-III)+uPAR(II-III) after 2cycles of chemotherapy was associated with poor survival (HR=1.79, 95% CI:1.08-2.97, p=0.023, n=57). This predictor, however, was not significant in the test set (p=0.21, 26 events in 27 patients). CONCLUSION: The baseline level of uPAR(I-III)+uPAR(II-III) is a predictor of survival in inoperable......BACKGROUND: High levels of intact and cleaved forms of the urokinase-type plasminogen activator receptor (uPAR) in both tissue and blood are associated with poor survival in several cancer diseases. The prognostic significance of uPAR in cholangiocarcinoma is unknown. The aims of this study were to...

  12. Cigarette Smoke and the Induction of Urokinase Plasminogen Activator Receptor In Vivo: Selective Contribution of Isoforms to Bronchial Epithelial Phenotype.

    Science.gov (United States)

    Portelli, Michael A; Stewart, Ceri E; Hall, Ian P; Brightling, Christopher E; Sayers, Ian

    2015-08-01

    The urokinase plasminogen activator receptor (uPAR) gene (PLAUR) has been identified as an asthma susceptibility gene, with polymorphisms within that gene being associated with baseline lung function, lung function decline, and lung function in a smoking population. Soluble cleaved uPAR (scuPAR), a molecule identified as a marker of increased morbidity and mortality in a number of diseases, has been shown to be elevated in the airways of patients with asthma and in patients with chronic obstructive pulmonary disease. However, the functionality of soluble receptor isoforms and their relationship with an important initiator for obstructive lung disease, cigarette smoke, remains undefined. In this study, we set out to determine the effect of cigarette smoke on soluble uPAR isoforms, its regulatory pathway and the resultant effect on bronchial epithelial cell function. We identified a positive association between cigarette pack-years and uPAR expression in the airway bronchial epithelium of biopsies from patients with asthma (n = 27; P = 0.0485). In vitro, cigarette smoke promoted cleavage of uPAR from the surface of bronchial epithelial cells (1.5× induction; P bronchial epithelial cells. This suggests that cigarette smoke elevates soluble receptor isoforms in bronchial epithelial cells through direct (cleavage) and indirect (messenger RNA expression) means. These findings provide further insight into how cigarette smoke may influence changes in the airways of importance to airway remodeling and obstructive lung disease progression. PMID:25490122

  13. Structure-based Engineering of Species Selectivity in the Interaction Between Urokinase and its Receptor: Implication for Preclinical Cancer Therapy

    International Nuclear Information System (INIS)

    The high affinity interaction between the urokinase-type plasminogen activator (uPA) and its glycolipid-anchored receptor (uPAR) is decisive for cell surface-associated plasminogen activation. Because plasmin activity controls fibrinolysis in a variety of pathological conditions, including cancer and wound healing, several intervention studies have focused on targeting the uPA · uPAR interaction in vivo. Evaluations of such studies in xenotransplanted tumor models are, however, complicated by the pronounced species selectivity in this interaction. We now report the molecular basis underlying this difference by solving the crystal structure for the murine uPA · uPAR complex and demonstrate by extensive surface plasmon resonance studies that the kinetic rate constants for this interaction can be swapped completely between these orthologs by exchanging only two residues. This study not only discloses the structural basis required for a successful rational design of the species selectivity in the uPA · uPAR interaction, which is highly relevant for functional studies in mouse models, but it also suggests the possible development of general inhibitors that will target the uPA · uPAR interaction across species barriers.

  14. Comparison of Video-Assisted Thoracoscopic Surgery and Intrapleural Urokinase as an Initial Treatment for Parapneumonic Effusion and Thoracic Empyema

    Directory of Open Access Journals (Sweden)

    Shungo Yukumi

    2014-05-01

    Full Text Available Introduction: The treatment of complicated parapneumonic effusion (PPE and thoracic empyema (TE is controversial; and the choice of treatment after confirming the failure of simple drainage remains unclear. The purpose of this study was to compare the outcomes of intrapleural urokinase (UK administration and video-assisted thoracoscopic surgery (VATS as initial treatment options for PPE and TE. Materials and Methods: We retrospectively reviewed and compared the data of 20 patients with PPE and TE diagnosed between January 2010 and December 2012 at our hospital, dividing them on the basis of the initial treatment into a video-assisted thoracoscopic surgery (VATS group (n=9 and UK group (n=11. Results: Age was the only statistically different parameter between both groups (P=0.025; with the mean age of the VATS and UK groups being 64 and 76 years, respectively. There was no significant difference in the duration of drainage or success rate between the UK or VATS groups. Although no statistically significant differences (P=0.20 were observed, duration of hospital stay was longer in the UK group (21 and 28 day for VATS and UK, respectively. Conclusion: VATS for PPE and TE may shorten the duration of hospital stay.However, UK administration may be used for selective patients because it is considered to yield outcomes similar to VATS.

  15. The Clinical Value of Soluble Urokinase Plasminogen Activator Receptor (suPAR) Levels in Autoimmune Connective Tissue Disorders.

    Science.gov (United States)

    Vasarhelyi, Barna; Toldi, Gergely; Balog, Attila

    2016-04-01

    The assessment of the general inflammatory condition of patients with autoimmune connective tissue disorders (ACTD) is a major challenge. The use of traditional inflammatory markers including CRP-levels and erythrocyte sedimentation rate (ESR) is limited by several preanalytical factors and their low specificities. Soluble urokinase plasminogen activator receptor (suPAR) is one of the novel candidate markers that is increasingly used in immune mediated disorders. In our studies we compared suPAR levels of patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), systemic sclerosis (SSc) and ankylosing spondylitis with those of healthy controls. suPAR provided valuable clinical information on disease activity in RA, SLE and SSc. We identified a subgroup of remitted RA patients, who presented still clinical symptoms of inflammatory activity which correlated to high plasma suPAR (while ESR and CRP were normal). In SLE we established specific suPAR cut-off values that support the discrimination between patients with high and those with moderate SLE activity. In patients with SSc suPAR correlated with objective measures of lung and other complications. In the majority of ACTDs including SLE, SSc or RA, suPAR is seemingly a good biomarker that would provide valuable clinical information. However, before the introduction of this novel parameter in laboratory repertoire important issues should be elucidated. These include the establishment of appropriate and disease specific cutoff values, clarification of interfering preanalytical values and underlying conditions and declaration of age- and gender-specific reference ranges. PMID:27683525

  16. MULTILATERAL DIPLOMACY AND INTERNATIONAL REGIMES

    Directory of Open Access Journals (Sweden)

    BENEA Ciprian-Beniamin

    2014-12-01

    Full Text Available The history of diplomacy can by divided in three main periods: one is that of occasional diplomacy peculiar to Middle Ages, while other belong to permane diplomacy, peculiar to modern times. But this one can be divided in two parts, too: one with a bilateral character, previos to 1st World War, and one with a multilateral character, manifested especially after the end on 1st World War. This third type is the focus of present paper. And it cannot be separated from the newly international constructs: international regimes, and international organizations. International instritutions the area where international regimes are belonging to – are legal constructs which provide the formal (and legal framework for continous negotiations. They are the most visible part of the new diplomacy – the one which has a permanent character, and it has an more open face. Anyway, the most important connection has to do with the international institutions, international regimes, and multilateral international negotiations. In the era of the new diplomacy, they all have a permanent character. International institutions help international negotiations carring on; while in their turn, they provide the base for international regimes’ creation, and especially for their evolution. The international regimes’ evolution is an inseparable part of a permanent international framework. And if there is missing a permanent international framework (international organization connected to a specific regime, this regime is a difuse one, its members have only informal relations among them, while they survey each other, looking at their behavior, but they don’t have a formal relationship among them, which could help them solving their future common interests, and protect them from their common fears. International regimes are very important in the era when evrithing touches, and influences everything. In the same time, the complexity of our present world can be successfully

  17. Great Lakes' regional climate regimes

    Science.gov (United States)

    Kravtsov, Sergey; Sugiyama, Noriyuki; Roebber, Paul

    2016-04-01

    We simulate the seasonal cycle of the Great Lakes' water temperature and lake ice using an idealized coupled lake-atmosphere-ice model. Under identical seasonally varying boundary conditions, this model exhibits more than one seasonally varying equilibrium solutions, which we associate with distinct regional climate regimes. Colder/warmer regimes are characterized by abundant/scarce amounts of wintertime ice and cooler/warmer summer temperatures, respectively. These regimes are also evident in the observations of the Great Lakes' climate variability over recent few decades, and are found to be most pronounced for Lake Superior, the deepest of the Great Lakes, consistent with model predictions. Multiple climate regimes of the Great Lakes also play a crucial role in the accelerated warming of the lakes relative to the surrounding land regions in response to larger-scale global warming. We discuss the physical origin and characteristics of multiple climate regimes over the lakes, as well as their implications for a longer-term regional climate variability.

  18. Cloud regimes as phase transitions

    Science.gov (United States)

    Stechmann, Samuel N.; Hottovy, Scott

    2016-06-01

    Clouds are repeatedly identified as a leading source of uncertainty in future climate predictions. Of particular importance are stratocumulus clouds, which can appear as either (i) closed cells that reflect solar radiation back to space or (ii) open cells that allow solar radiation to reach the Earth's surface. Here we show that these clouds regimes -- open versus closed cells -- fit the paradigm of a phase transition. In addition, this paradigm characterizes pockets of open cells as the interface between the open- and closed-cell regimes, and it identifies shallow cumulus clouds as a regime of higher variability. This behavior can be understood using an idealized model for the dynamics of atmospheric water as a stochastic diffusion process. With this new conceptual viewpoint, ideas from statistical mechanics could potentially be used for understanding uncertainties related to clouds in the climate system and climate predictions.

  19. Urokinase-type plasminogen activator deficiency has little effect on seizure susceptibility and acquired epilepsy phenotype but reduces spontaneous exploration in mice.

    Science.gov (United States)

    Rantala, J; Kemppainen, S; Ndode-Ekane, X E; Lahtinen, L; Bolkvadze, Tamuna; Gurevicius, K; Tanila, H; Pitkänen, A

    2015-01-01

    Urokinase-type plasminogen activator (uPA), a serine protease, converts plasminogen to plasmin. Activation of plasmin leads to degradation of the extracellular matrix, which is critical for tissue recovery, angiogenesis, cell migration, and axonal and synaptic plasticity. We hypothesized that uPA deficiency would cause an abnormal neurophenotype and would lead to exacerbated epileptogenesis after brain injury. Wild-type (Wt) and uPA-/- mice underwent a battery of neurologic behavioral tests evaluating general reactivity, spontaneous exploratory activity, motor coordination, pain threshold, fear and anxiety, and memory. We placed particular emphasis on the effect of uPA deficiency on seizure susceptibility, including the response to convulsants (pentylenetetrazol, kainate, or pilocarpine) and kainate-induced epileptogenesis and epilepsy. The uPA-/- mice showed no motor or sensory impairment compared with the Wt mice. Hippocampus-dependent spatial memory also remained intact. The uPA-/- mice, however, exhibited reduced exploratory activity and an enhanced response to a tone stimulus (p<0.05 compared with the Wt mice). The urokinase-type plasminogen activator deficient mice showed no increase in spontaneous or evoked epileptiform electrographic activity. Rather, the response to pilocarpine administration was reduced compared with the Wt mice (p<0.05). Also, the epileptogenesis and the epilepsy phenotype after intrahippocampal kainate injection were similar to those in the Wt mice. Taken together, uPA deficiency led to diminished interest in the environmental surroundings and enhanced emotional reactivity to unexpected aversive stimuli. Urokinase-type plasminogen activator deficiency was not associated with enhanced seizure susceptibility or worsened poststatus epilepticus epilepsy phenotype.

  20. Friction Regimes in the Lubricants Solid-State Regime

    NARCIS (Netherlands)

    Schipper, D.J.; Maathuis, O.; Dowson, D.; Taylor, C.M.; Childs, T.H.C.; Dalmaz, G.

    1995-01-01

    Friction measurements were performed in the lubricant's solid-state regime to study the transition from full-film lubrication, in which the separation is maintained by a solidified lubricant, to mixed lubrication. Special attention is paid to the influence of temperature (inlet viscosity) and roughn

  1. On the regimes of premixing

    Energy Technology Data Exchange (ETDEWEB)

    Angelini, S.; Theofanous, T.G.; Yuen, W.W. [California Univ., Santa Barbara, CA (United States). Center for Risk Studies and Safety

    1998-01-01

    The conditions of the MAGICO-2000 experiment are extended to more broadly investigate the regimes of premixing, and the corresponding internal structures of mixing zones. With the help of the data and numerical simulations using the computer code PM-ALPHA, we can distinguish extremes of behavior dominated by inertia and thermal effects - we name these the inertia and thermal regimes, respectively. This is an important distinction that should guide future experiments aimed at code verification in this area. Interesting intermediate behaviors are also delineated and discussed. (author)

  2. Renin angiotensin system blockade reduces urinary levels of soluble urokinase plasminogen activator receptor (suPAR) in patients with type 2 diabetes

    DEFF Research Database (Denmark)

    Persson, Frederik; Theilade, Simone; Eugen-Olsen, Jesper;

    2016-01-01

    angiotensin system (RAS) single and dual blockade on suPAR levels in patients with type 2 diabetes and albuminuria. We conducted a post-hoc analysis of a randomized controlled crossover trial. Urine and plasma samples were analyzed for suPAR levels. The placebo period was considered reference and all......Soluble urokinase plasminogen activator receptor (suPAR) is associated with faster decline in kidney function and the pathogenesis of diabetic nephropathy. However, little is known about the impact of treatment on plasma and urinary levels of suPAR. We aimed to investigate the impact of renin...

  3. Prognostic value of intact and cleaved forms of the urokinase plasminogen activator receptor in a retrospective study of 518 colorectal cancer patients

    DEFF Research Database (Denmark)

    Lomholt, Anne Fog; Christensen, Ib J; Høyer-Hansen, Gunilla;

    2010-01-01

    The levels of the soluble urokinase plasminogen activator receptor (suPAR) in blood have been shown to correlate with prognosis in various cancers. Plasma levels of the combined suPAR forms have previously shown to be a strong prognostic marker in the present cohort of CRC patients and could...... potentially identify high-risk patients among those with early stage disease. In order to investigate whether the individual suPAR forms are stronger prognostic markers than the combined amount we measured the different uPAR forms in serum from the same cohort and evaluated their prognostic significance....

  4. Soluble Urokinase Plasminogen Activator Receptor (suPAR) is Associated with Metabolic Changes in HIV-1-Infected Africans: A Prospective Study

    DEFF Research Database (Denmark)

    Fourie, Carla M T; Van Rooyen, Johannes M; Olsen, Michael H;

    2011-01-01

    Soluble urokinase plasminogen activator receptor (suPAR) is associated with inflammation and may predict lipodystrophy and dysmetabolism in human immunodeficiency virus (HIV)-infected individuals on antiretroviral therapy. We aimed to assess firstly, whether suPAR levels are elevated in treated......-infected black South Africans had significantly higher suPAR levels than uninfected controls at baseline and at follow-up 3 years later. However, only the treated HIV-1-infected participants showed an increase in suPAR levels at follow-up. The treated group also showed signs of lipodystrophy...

  5. Lysosomal degradation of receptor-bound urokinase-type plasminogen activator is enhanced by its inhibitors in human trophoblastic choriocarcinoma cells

    DEFF Research Database (Denmark)

    Jensen, Poul Henning; Christensen, Erik Ilsø; Ebbesen, P.;

    1990-01-01

    We have studied the effect of plasminogen activator inhibitors PAI-1 and PAI-2 on the binding of urokinase-type plasminogen activator (u-PA) to its receptor in the human choriocarcinoma cell line JAR. With 125I-labeled ligands in whole-cell binding assays, both uncomplexed u-PA and u-PA-inhibitor...... for the removal of u-PA after its complex formation with a specific inhibitor. The data suggest a novel mechanism by which receptor-mediated endocytosis is initiated by the binding of a secondary ligand. Full text...

  6. Direct interaction of the kringle domain of urokinase-type plasminogen activator (uPA) and integrin alpha v beta 3 induces signal transduction and enhances plasminogen activation

    OpenAIRE

    Tarui, Takehiko; Akakura, Nobuaki; Majumdar, Mousumi; Andronicos, Nicholas; Takagi, Junichi; Mazar, Andrew P.; Bdeir, Khalil; Kuo, Alice; Yarovoi, Serge V.; Cines, Douglas B.; Takada, Yoshikazu

    2006-01-01

    It has been questioned whether there are receptors for urokinase-type plasminogen activator (uPA) that facilitate plasminogen activation other than the high affinity uPA receptor (uPAR/CD87) since studies of uPAR knockout mice did not support a major role of uPAR in plasminogen activation. uPA also promotes cell adhesion, chemotaxis, and proliferation besides plasminogen activation. These uPA-induced signaling events are not mediated by uPAR, but mediated by unidentified, lower-affinity recep...

  7. Catheter-Directed Thrombolysis with a Continuous Infusion of Low-Dose Urokinase for Non-Acute Deep Venous Thrombosis of the Lower Extremity

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Binbin; Zhang, Jingyong; Wu, Xuejun; Han, Zonglin; Zhou, Hua; Dong, Dianning; Jin, Xing [Shandong Provincial Hospital, Shandong University, Ji' nan (China)

    2011-02-15

    We wanted to evaluate the feasibility of catheter-directed thrombolysis with a continuous infusion of low-dose urokinase for treating non-acute (less than 14 days) deep venous thrombosis of the lower extremity. The clinical data of 110 patients who were treated by catheter-directed thrombolysis with a continuous infusion of low-dose urokinase for lower extremity deep venous thrombosis was analysed. Adjunctive angioplasty or/and stenting was performed for the residual stenosis. Venous recanalization was graded by pre- and posttreatment venography. Follow-up was performed by clinical evaluation and Doppler ultrasound. A total of 112 limbs with deep venous thrombosis with a mean symptom duration of 22.7 days (range: 15-38 days) were treated with a urokinase infusion (mean: 3.5 million IU) for a mean of 196 hours. After thrombolysis, stent placement was performed in 25 iliac vein lesions and percutaneous angioplasty (PTA) alone was done in fi ve iliac veins. Clinically significant recanalization was achieved in 81% (90 of 112) of the treated limbs: complete recanalization was achieved in 28% (31 of 112) and partial recanalization was achieved in 53% (59 of 112). Minor bleeding occurred in 14 (13%) patients, but none of the patients suffered from major bleeding or symptomatic pulmonary embolism. During followup (mean: 15.2 months, range: 3-24 months), the veins were patent in 74 (67%) limbs. Thirty seven limbs (32%) showed progression of the stenosis with luminal narrowing more than 50%, including three with rethrombosis, while one revealed an asymptomatic iliac vein occlusion: 25 limbs (22%) developed mild post-thrombotic syndrome, and none had severe post-thrombotic syndrome. Valvular reflux occurred in 24 (21%) limbs. Catheter-directed thrombolysis with a continuous infusion of low-dose urokinase combined with adjunctive iliac vein stenting is safe and effective for removal of the clot burden and for restoration of the venous flow in patients with non-acute lower

  8. Mapping the topographic epitope landscape on the urokinase plasminogen activator receptor (uPAR) by surface plasmon resonance and X-ray crystallography

    OpenAIRE

    Baoyu Zhao; Sonu Gandhi; Cai Yuan; Zhipu Luo; Rui Li; Henrik Gårdsvoll; Valentina de Lorenzi; Nicolai Sidenius; Mingdong Huang; Michael Ploug

    2015-01-01

    The urokinase-type plasminogen activator receptor (uPAR or CD87) is a glycolipid-anchored membrane protein often expressed in the microenvironment of invasive solid cancers and high levels are generally associated with poor patient prognosis (Kriegbaum et al., 2011 [1]). uPAR is organized as a dynamic modular protein structure composed of three homologous Ly6/uPAR domains (LU).This internally flexible protein structure of uPAR enables an allosteric regulation of the interactions with its two ...

  9. Proteomic Identification of LASP-1 Down-regulation After RNAi Urokinase Silencing in Human Hepatocellular Carcinoma Cells

    Directory of Open Access Journals (Sweden)

    Alessandro Salvi

    2009-02-01

    Full Text Available In human hepatocellular carcinoma (HCC, the high expression of urokinase-type plasminogen activator (uPA is an unfavorable prognostic factor and a therapeutic target. To identify the downstream effects of uPA silencing by RNA interference, we studied proteome modifications of uPA-inhibited SKHep1C3 cells, an HCC-derived cell line. The study with two-dimensional difference gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry showed Lim and SH3 protein 1 (LASP-1, cytokeratin 1 (CK-1, cytokeratin 10 (CK-10, and heterogeneous nuclear ribonucleoprotein H1 down-modulation after uPA inhibition. LASP-1, CK-1, and CK-10 are involved in cytoskeleton dynamics as heterogeneous nuclear ribonucleoprotein H1 takes part in the mRNA processing and stability. We first confirmed the proteomic data by Western blot and immunoflorescence and then explored the link between uPA and LASP-1. The ectopic expression of uPA and LASP-1 supported the proteomic results and showed that uPA up-regulation increased LASP-1 expression and that both were implicated in SKHep1C3 motility. siRNA LASP-1 inhibition showed that LASP-1 was involved in actin microfilaments organization of SKHep1C3 cells. The disruption of the actin microfilaments after LASP-1 depletion increased uPA secretion and SKHep1C3 motility. Our results would suggest the hypothesis that uPA and LASP-1 expression may be coordinated in HCC-derived cells. In summary, the proteomic identification of a set of uPA downstream proteins provides new insight into the function of uPA in HCC cells.

  10. CFTR suppresses tumor progression through miR-193b targeting urokinase plasminogen activator (uPA) in prostate cancer.

    Science.gov (United States)

    Xie, C; Jiang, X H; Zhang, J T; Sun, T T; Dong, J D; Sanders, A J; Diao, R Y; Wang, Y; Fok, K L; Tsang, L L; Yu, M K; Zhang, X H; Chung, Y W; Ye, L; Zhao, M Y; Guo, J H; Xiao, Z J; Lan, H Y; Ng, C F; Lau, K M; Cai, Z M; Jiang, W G; Chan, H C

    2013-05-01

    Cystic fibrosis (CF) transmembrane conductance regulator (CFTR) is expressed in the epithelial cells of a wide range of organs/tissues from which most cancers are derived. Although accumulating reports have indicated the association of cancer incidence with genetic variations in CFTR gene, the exact role of CFTR in cancer development and the possible underlying mechanism have not been elucidated. Here, we report that CFTR expression is significantly decreased in both prostate cancer cell lines and human prostate cancer tissue samples. Overexpression of CFTR in prostate cancer cell lines suppresses tumor progression (cell growth, adhesion and migration), whereas knockdown of CFTR leads to enhanced malignancies both in vitro and in vivo. In addition, we demonstrate that CFTR knockdown-enhanced cell proliferation, cell invasion and migration are significantly reversed by antibodies against either urokinase plasminogen activator (uPA) or uPA receptor (uPAR), which are known to be involved in various malignant traits of cancer development. More interestingly, overexpression of CFTR suppresses uPA by upregulating the recently described tumor suppressor microRNA-193b (miR-193b), and overexpression of pre-miR-193b significantly reverses CFTR knockdown-enhanced malignant phenotype and abrogates elevated uPA activity in prostate cancer cell line. Finally, we show that CFTR gene transfer results in significant tumor repression in prostate cancer xenografts in vivo. Taken together, the present study has demonstrated a previously undefined tumor-suppressing role of CFTR and its involvement in regulation of miR-193b in prostate cancer development. PMID:22797075

  11. Plasminogen activator inhibitor-1 (PAI-1 and urokinase plasminogen activator (uPA in sputum of allergic asthma patients.

    Directory of Open Access Journals (Sweden)

    Sebastian Zukowski

    2008-06-01

    Full Text Available Urokinase plasminogen activator (uPA and its inhibitor (PAI-1 have been associated with asthma. The aim of this study was to evaluate concentration of uPA and PAI-1 in induced sputum of house dust mite allergic asthmatics (HDM-AAs. The study was performed on 19 HDM-AAs and 8 healthy nonatopic controls (HCs. Concentration of uPA and PAI-1 was evaluated in induced sputum supernatants using ELISA method. In HDM-AAs the median sputum concentration of uPA (128 pg/ml; 95% CI 99 to 183 pg/ml and PAI-1 (4063 pg/ml; 95%CI 3319 to 4784 pg/ml were significantly greater than in HCs (17 pg/ml; 95%CI 12 to 32 pg/ml; p<0.001 and 626 pg/ml; 95%CI 357 to 961 pg/ml; p<0.001 for uPA and PAI-1 respectively. The sputum concentration of uPA correlated with sputum total cell count (r=0.781; p=0.0001 and with logarithmically transformed exhaled nitric oxide concentration (eNO (r=0.486; p=0.035 but not with FEV1 or bronchial reactivity to histamine. On the contrary, the sputum PAI-1 concentration correlated with FEV1 (r=-0,718; p=0.0005 and bronchial reactivity to histamine expressed as log(PC20 (r=-0.824; p<0.0001 but did not correlate with sputum total cell count or eNO. The results of this study support previous observations linking PAI-1 with airway remodeling and uPA with cellular inflammation. Moreover, the observed effect of uPA seems to be independent of its fibrynolytic activity.

  12. Flow cytometry evaluation of urokinase-type plasminogen activator receptor (UPA-R) in acute myeloid leukemia cells.

    Science.gov (United States)

    Castagnari, B; Moretti, S; Latorraca, A; Rigolin, G M; Balsamo, R; Lanza, F; Castoldi, G L

    1995-01-01

    The aim of this study was to investigate by flow cytometry the expression of the UPA-R (Urokinase type plasminogen activator receptor-CD87) on the blastic population of AML and ALL patients in order to evaluate whether the presence of this molecule could be associated with peculiar clinical and biologic features of leukemic cells. Five different monoclonal antibodies (MoAbs) (clones: 3B10#; VIM5*; 109#; 68#; 100#) were used in order to detect the distinct forms of this cellular receptor. Cell reactivity varied significantly from case to case, also depending on the MoAb used for the flow cytometry analysis. In brief, 3B10# and VIM5* MoAbs were found to be positive in more than 90% of monocytes and neutrophils from healthy subjects, while the number of positive cells was decreased (60%) using the 109# MoAb. However, either 68# and 100# MoAbs recognised only a low number of blood monocytes and neutrophils (8-20%), while lymphocytes were unreactive with all the five UPA-R MoAbs. ALL cells were found to be CD87 negative in all cases. Blasts from AML showed a heterogeneous pattern of expression for the UPA-R MoAbs, being the reactivity strictly dependent on the MoAb used, and, to a higher extent, on the degree and type of maturation of the blastic cells. The number of blasts recognising 3B10# and VIM5* MoAbs was significantly higher than that reacting with the remaining MoAbs irrespective of the FAB subtype. Since proteolytic enzymes, like UPA, play a key role in the dissolution of the extracellular matrix, and in facilitating the cell egress from the bone marrow, it is conceivable that the expression of the UPA-R could contribute to the invasive properties and, possibly, metastatic potential of leukemic cells. PMID:8519488

  13. Monetary regimes in open economies

    NARCIS (Netherlands)

    Korpos, A.

    2006-01-01

    This thesis presents a two-country open economy framework for the analysis of strategic interactions among monetary authorities and wage bargaining institutions. From this perspective, the thesis investigates the economic consequences of replacing flexible and fixed exchange rate regimes with a mone

  14. Friction Regimes in the Lubricants Solid-State Regime

    OpenAIRE

    Schipper, D.J.; Maathuis, O.; Dowson, D; Taylor, C.M.; Childs, T.H.C.; Dalmaz, G.

    1995-01-01

    Friction measurements were performed in the lubricant's solid-state regime to study the transition from full-film lubrication, in which the separation is maintained by a solidified lubricant, to mixed lubrication. Special attention is paid to the influence of temperature (inlet viscosity) and roughness on this transition. The friction measurements showed that in the lubricants solid-state region three lubrication modes can be distinguished: A) full-film lubrication; separation is maintained b...

  15. Inhibitory effect of berberine on the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2

    International Nuclear Information System (INIS)

    Berberine, a compound isolated from medicinal herbs, has been reported with many pharmacological effects related to anti-cancer and anti-inflammation capabilities. In this study, we observed that berberine exerted a dose- and time-dependent inhibitory effect on the motility and invasion ability of a highly metastatic A549 cells under non-cytotoxic concentrations. In cancer cell migration and invasion process, matrix-degrading proteinases are required. A549 cell treated with berberine at various concentrations showed reduced ECM proteinases including matrix metalloproteinase-2 (MMP2) and urokinase-plasminogen activator (u-PA) by gelatin and casein zymography analysis. The inhibitory effect is likely to be at the transcriptional level, since the reduction in the transcripts levels was corresponding to the proteins. Moreover, berberine also exerted its action via regulating tissue inhibitor of metalloproteinase-2 (TIMP-2) and urokinase-plasminogen activator inhibitor (PAI). The upstream mediators of the effect involved c-jun, c-fos and NF-κB, as evidenced by reduced phosphorylation of the proteins. These findings suggest that berberine possesses an anti-metastatic effect in non-small lung cancer cell and may, therefore, be helpful in clinical treatment

  16. Urokinase plasminogen receptor and the fibrinolytic complex play a role in nerve repair after nerve crush in mice, and in human neuropathies.

    Directory of Open Access Journals (Sweden)

    Cristina Rivellini

    Full Text Available Remodeling of extracellular matrix (ECM is a critical step in peripheral nerve regeneration. In fact, in human neuropathies, endoneurial ECM enriched in fibrin and vitronectin associates with poor regeneration and worse clinical prognosis. Accordingly in animal models, modification of the fibrinolytic complex activity has profound effects on nerve regeneration: high fibrinolytic activity and low levels of fibrin correlate with better nerve regeneration. The urokinase plasminogen receptor (uPAR is a major component of the fibrinolytic complex, and binding to urokinase plasminogen activator (uPA promotes fibrinolysis and cell movement. uPAR is expressed in peripheral nerves, however, little is known on its potential function on nerve development and regeneration. Thus, we investigated uPAR null mice and observed that uPAR is dispensable for nerve development, whereas, loss of uPAR affects nerve regeneration. uPAR null mice showed reduced nerve repair after sciatic nerve crush. This was a consequence of reduced fibrinolytic activity and increased deposition of endoneurial fibrin and vitronectin. Exogenous fibrinolysis in uPAR null mice rescued nerve repair after sciatic nerve crush. Finally, we measured the fibrinolytic activity in sural nerve biopsies from patients with peripheral neuropathies. We showed that neuropathies with defective regeneration had reduced fibrinolytic activity. On the contrary, neuropathies with signs of active regeneration displayed higher fibrinolytic activity. Overall, our results suggest that enforced fibrinolysis may facilitate regeneration and outcome of peripheral neuropathies.

  17. The Concept of Truth Regime

    Directory of Open Access Journals (Sweden)

    Lorna Weir

    2008-07-01

    Full Text Available “Truth regime” is a much used but little theorized concept, with the Foucauldian literature presupposing that truth in modernity is uniformly scientific/quasi-scientific and enhances power. I argue that the forms of truth characteristic of our present are wider than Foucault recognized, their relations to power more various, and their historicity more complex. The truth regime of advanced modernity is characterized by multiple, irreducible truth formulae that co-exist and sometimes vie for dominance. A truth formula stabilizes a network of elements: a relation between representation and presentation (words and things, truth and non-truth, and the place of the subject in discourse. Our contemporary truth regime comprises radically heterogeneous truthful knowledges – science, governance, religion/politics, and common culture – that have distinct histories and relations to power.

  18. Demystifying optimal dynamic treatment regimes.

    Science.gov (United States)

    Moodie, Erica E M; Richardson, Thomas S; Stephens, David A

    2007-06-01

    A dynamic regime is a function that takes treatment and covariate history and baseline covariates as inputs and returns a decision to be made. Murphy (2003, Journal of the Royal Statistical Society, Series B 65, 331-366) and Robins (2004, Proceedings of the Second Seattle Symposium on Biostatistics, 189-326) have proposed models and developed semiparametric methods for making inference about the optimal regime in a multi-interval trial that provide clear advantages over traditional parametric approaches. We show that Murphy's model is a special case of Robins's and that the methods are closely related but not equivalent. Interesting features of the methods are highlighted using the Multicenter AIDS Cohort Study and through simulation. PMID:17688497

  19. Quantitative RT-PCR assays for the determination of urokinase-type plasminogen activator and plasminogen activator inhibitor type 1 mRNA in primary tumor tissue of breast cancer patients: comparison to antigen quantification by ELISA.

    NARCIS (Netherlands)

    Biermann, J.C.; Holzscheiter, L.; Kotzsch, M.; Luther, T.; Kiechle-Bahat, M.; Sweep, F.C.; Span, P.N.; Schmitt, M.; Magdolen, V.

    2008-01-01

    Urokinase-type plasminogen activator (uPA) and its inhibitor plasminogen activator inhibitor type 1 (PAI-1) play a key role in tumor-associated processes such as the degradation of extracellular matrix proteins, tissue remodeling, cell adhesion, migration, and invasion. High antigen levels of uPA an

  20. The EU Commission consultation regime

    OpenAIRE

    Quittkat, Christine; Finke, Barbara

    2008-01-01

    "The present consultation regime of the European Commission is marked by the role the Commission assigns to non-governmental actors or civil society organisations (CSOs). The Commission's documents on its policy of consultation and cooperation with external non-governmental actors reveal that a reflective approach has emerged during the 1980s, referring to a more elaborate concept of 'good governance'. The gradual extension is most noticeable in the change of terminology, from ‘consultation’ ...

  1. The International Climate Change Regime

    Science.gov (United States)

    Yamin, Farhana; Depledge, Joanna

    2005-01-01

    Aimed at the increasing number of policy-makers, stakeholders, researchers, and other professionals working on climate change, this volume presents a detailed description and analysis of the international regime established in 1992 to combat the threat of global climate change. It provides a comprehensive accessible guide to a high-profile area of international law and politics, covering not only the obligations and rights of countries, but ongoing climate negotiations as well.

  2. Disclosure regimes and corporate governance

    OpenAIRE

    Wüstemann, Jens

    2004-01-01

    As there is a strong relationship of disclosure regimes and corporate governance the internationally varying distribution of information rights can be interpreted as a function of different systems of corporate governance. A disclosure system comprises all legally recognized information claims that a system of corporate governance or a financial system furnishes financial contracting parties with: Both public disclosure and disclosure via private information channels may serve value-relevant ...

  3. Exchange Rate Regimes and Location

    OpenAIRE

    Ricci, Luca Antonio

    1997-01-01

    This paper investigates the effects of fixed versus flexible exchange rate regimes on location choices of firms and on the degree of specialization of countries. In a two-country two-differentiated-good monetary model, demand, supply, and monetary shocks arise after wages are set and prices are optimally chosen. The exchange rate performs then an adjustment role for firms located in the country relatively specialized in the good they produce, but it constitutes a factor of disturbance for the...

  4. Exchange rate regimes and monetary arrangements

    Directory of Open Access Journals (Sweden)

    Ivan Ribnikar

    2005-06-01

    Full Text Available There is a close relationship between a country’s exchange rate regime and monetary arrangement and if we are to examine monetary arrangements then exchange rate regimes must first be analysed. Within the conventional and most widely used classification of exchange rate regimes into rigid and flexible or into polar regimes (hard peg and float on one side, and intermediate regimes on the other there, is a much greater variety among intermediate regimes. A more precise and, as will be seen, more useful classification of exchange rate regimes is the first topic of the paper. The second topic is how exchange rate regimes influence or determine monetary arrangements and monetary policy or monetary policy regimes: monetary autonomy versus monetary nonautonomy and discretion in monetary policy versus commitment in monetary policy. Both topics are important for countries on their path to the EU and the euro area

  5. Merging of Rhine flow regimes

    Science.gov (United States)

    Boessenkool, Berry; Bronstert, Axel; Bürger, Gerd

    2016-04-01

    The Rhine flow regime is changing: (a) in the alpine nival regime, snow melt floods occur earlier in the year and (b) in the pluvial middle-Rhine regime, rainfall induced flood magnitudes rise. The seasonality of each is currently separated in time, but it is conceivable that this may shift due to climate change. If extremes of both flood types coincide, this would create a new type of hydrologic extreme with disastrous consequences. Quantifying the probability for a future overlap of pluvial and nival floods is therefore of high relevance to society and particularly to reinsurance companies. In order to investigate possible changes in magnitude and timing of flood types, we are developing a chain of physical models for spatio-temporal combination of flood probabilities. As input, we aim to use stochastically downscaled temperature and rainfall extremes from climate model weather projections. Preliminary research shows a six-week forward-shift of peak discharge at the nival gauge Maxau in the past century. The aim of presenting our early-stage work as a poster is to induce an exchange of ideas with fellow scientists in close research disciplines.

  6. Role of Urokinase-type Plasminogen Activator in the Precontact Sperm-egg Communication and Fertility of Mice in vitro

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Objective To explore the role of urokinase-type plasminogen activator(uPA) in precontact sperm-egg communication and fertility of mice in vitro.Methods Firstly, sperm chemotaxis (SC) induced by uPA was assayed by measuring the sperm densities in capillaries with a descending gradient or no gradient of uPA respectively. Secondly, the role of uPAR that exists in sperm plasma membrane in SC was studied by examining the change of sperm density in capillary after incubating spermatozoa with anti-uPAR antibody. Thirdly, SC induced by eggs, which had been treated with uPA, PAI-1 and anti-uPAR beforehand respectively, was assayed to study the role of uPA in PSEC. Lastly, the fertilization capability of spermatozoa treated with uPA was examined by counting the number of fertilized eggs.Results 1)The density of spermatozoa that migrated down the gradient of uPA into the capillary was significantly lower than that into the capillary containing no-gradient uPA. 2) When uPAR of spermatozoa was inhibited by anti-uPAR antibody, the density of spermatozoa that migrated into the capillary with ascending gradient of uPA decreased correspondingly. 3) The density of spermatozoa attracted by eggs, which were treated with uPA beforehand, increased significantly than that of attracted by non-treated eggs. On the contrary, the sperm density decreased correspondingly when the egg was treated with PAI-1. 4) The number of fertilized eggs increased significantly after the spermatozoa used here was treated with uPA beforehand.Conclusion uPA could induce SC of mice sperm in vitro through the uPAR on its membrane, enhance the capability of egg inducing SC, and promote spermatozoa to fertilize eggs. Thus, uPA may act as an attractant in PSEC, increase the chance encounter of spermatozoa and eggs, therefore, enhance the fertility success correspondingly.This study, in some degree, provides an evidence that uPA may be used as a new medicine and diagnostic reagent for male infertility.

  7. Plasma levels of soluble urokinase-type plasminogen activator receptor associate with the clinical severity of acute Puumala hantavirus infection.

    Directory of Open Access Journals (Sweden)

    Tuula K Outinen

    Full Text Available OBJECTIVES: Urokinase-type plasminogen activator receptor is a multifunctional glycoprotein, the expression of which is increased during inflammation. It is known to bind to β3-integrins, which are elementary for the cellular entry of hantaviruses. Plasma soluble form of the receptor (suPAR levels were evaluated as a predictor of severe Puumala hantavirus (PUUV infection and as a possible factor involved in the pathogenesis of the disease. DESIGN: A single-centre prospective cohort study. SUBJECTS AND METHODS: Plasma suPAR levels were measured twice during the acute phase and once during the convalescence in 97 patients with serologically confirmed acute PUUV infection using a commercial enzyme-linked immunosorbent assay (ELISA. RESULTS: The plasma suPAR levels were significantly higher during the acute phase compared to the control values after the hospitalization (median 8.7 ng/ml, range 4.0-18.2 ng/ml vs. median 4.7 ng/ml, range 2.4-12.2 ng/ml, P<0.001. The maximum suPAR levels correlated with several variables reflecting the severity of the disease. There was a positive correlation with maximum leukocyte count (r = 0.475, p<0.001, maximum plasma creatinine concentration (r = 0.378, p<0.001, change in weight during the hospitalization (r = 0.406, p<0.001 and the length of hospitalization (r = 0.325, p = 0.001, and an inverse correlation with minimum platelet count (r = -0.325, p = 0.001 and minimum hematocrit (r = -0.369, p<0.001. CONCLUSION: Plasma suPAR values are markedly increased during acute PUUV infection and associate with the severity of the disease. The overexpression of suPAR possibly activates β3-integrin in PUUV infection, and thus might be involved in the pathogenesis of the disease.

  8. Plasma Levels of Soluble Urokinase-Type Plasminogen Activator Receptor Associate with the Clinical Severity of Acute Puumala Hantavirus Infection

    Science.gov (United States)

    Outinen, Tuula K.; Tervo, Laura; Mäkelä, Satu; Huttunen, Reetta; Mäenpää, Niina; Huhtala, Heini; Vaheri, Antti; Mustonen, Jukka; Aittoniemi, Janne

    2013-01-01

    Objectives Urokinase-type plasminogen activator receptor is a multifunctional glycoprotein, the expression of which is increased during inflammation. It is known to bind to β3-integrins, which are elementary for the cellular entry of hantaviruses. Plasma soluble form of the receptor (suPAR) levels were evaluated as a predictor of severe Puumala hantavirus (PUUV) infection and as a possible factor involved in the pathogenesis of the disease. Design A single-centre prospective cohort study. Subjects and Methods Plasma suPAR levels were measured twice during the acute phase and once during the convalescence in 97 patients with serologically confirmed acute PUUV infection using a commercial enzyme-linked immunosorbent assay (ELISA). Results The plasma suPAR levels were significantly higher during the acute phase compared to the control values after the hospitalization (median 8.7 ng/ml, range 4.0–18.2 ng/ml vs. median 4.7 ng/ml, range 2.4–12.2 ng/ml, P<0.001). The maximum suPAR levels correlated with several variables reflecting the severity of the disease. There was a positive correlation with maximum leukocyte count (r = 0.475, p<0.001), maximum plasma creatinine concentration (r = 0.378, p<0.001), change in weight during the hospitalization (r = 0.406, p<0.001) and the length of hospitalization (r = 0.325, p = 0.001), and an inverse correlation with minimum platelet count (r = −0.325, p = 0.001) and minimum hematocrit (r = −0.369, p<0.001). Conclusion Plasma suPAR values are markedly increased during acute PUUV infection and associate with the severity of the disease. The overexpression of suPAR possibly activates β3-integrin in PUUV infection, and thus might be involved in the pathogenesis of the disease. PMID:23990945

  9. Prognostic value analysis of urokinase-type plasminogen activator receptor in oral squamous cell carcinoma: an immunohistochemical study

    Directory of Open Access Journals (Sweden)

    Rocchetti Romina

    2008-08-01

    Full Text Available Abstract Background Oral squamous cell carcinoma (OSCC represents the most common oral malignancy. Despite recent advances in therapy, up to 50% of the cases have relapse and/or metastasis. There is therefore a strong need for the identification of new biological markers able to predict the clinical behaviour of these lesions in order to improve quality of life and overall survival. Among tumour progression biomarkers, already known for their involvement in other neoplasia, a crucial role is ascribed to the urokinase-type plasminogen activator receptor (uPAR, which plays a multiple role in extracellular proteolysis, cell migration and tissue remodelling not only as a receptor for the zymogen pro-uPA but also as a component for cell adhesion and as a chemoattractant. The purpose of this study was to gain information on the expression of uPAR in OSCC and to verify whether this molecule can have a role as a prognostic/predictive marker for this neoplasia. Methods In a retrospective study, a cohort of 189 OSCC patients was investigated for uPAR expression and its cellular localization by immunohistochemistry. As standard controls, 8 normal oral mucosal tissues free of malignancy, obtained from patients with no evidence or history of oral cavity tumours, were similarly investigated. After grouping for uPAR expression, OSCCs were statistically analyzed for the variables age, gender, histological grading (G, tumour size, recurrence, TNM staging and overall survival rate. Results In our immunohistochemical study, 74 cases (39.1% of OSCC showed a mostly cytoplasmic positivity for uPAR, whereas 115 were negative. uPAR expression correlated with tumour differentiation grade and prognosis: percentage of positive cases was the greatest in G3 (70.4% and patients positives for uPAR expression had an expectation of life lower than those for uPAR negatives. Conclusion The results obtained in this study suggest a role of uPAR as a potential biomarker useful to

  10. Expression and functional role of urokinase-type plasminogen activator receptor in normal and acute leukaemic cells.

    Science.gov (United States)

    Lanza, F; Castoldi, G L; Castagnari, B; Todd, R F; Moretti, S; Spisani, S; Latorraca, A; Focarile, E; Roberti, M G; Traniello, S

    1998-10-01

    Urokinase-type plasminogen activator receptor (UPA-R-CD87) is a GPI-anchored membrane protein which promotes the generation of plasmin on the surface of many cell types, probably facilitating cellular extravasation and tissue invasion. A flow cytometric quantitative analysis of expression levels for UPA-R was performed on fresh blast cells from patients with acute myeloid leukaemia (AML, n = 74), acute lymphoblastic leukaemia (ALL, n = 24), and biphenotypic leukaemia (BAL, n = 3) using two CD87 monoclonal antibodies (McAbs) (3B10 and VIM5). Peripheral blood and bone marrow (BM) cells from 15 healthy adults served as controls. Using 3B10 McAb, UPA-R was expressed (>99%) by blood monocytes, neutrophils, and BM myelomonocytic precursors in controls, whereas resting T and B lymphocytes, and CD34+ cells were UPA-R negative. We also attempted to clarify whether UPA-R has a role in mediating neutrophil functions. Oriented locomotion induced by different chemotaxins and lysozyme release by granules stimulated with fMLP or PMA were significantly decreased when UPA-R was neutralized by CD87 McAb. In contrast, the anti-UPA-R McAb had no effect on superoxide anion generation of normal neutrophils. Blasts from AML showed a heterogenous pattern of expression for the UPA-R McAbs, with reactivity strictly dependent on FAB subtype. The highest UPA-R expression was seen in the M5 group: all patients tested (n = 20) showed strong positivity for the UPA-R McAb whereas only 12% (3/24) of ALL patients were CD87 positive, and 2/3 of BAL patients showed a dim expression for CD87. The number of receptors expressed by blast cells in 6/74 (8.1%) AML patients was higher than those of normal samples: in addition, since co-expression of UPA-R and CD34 was not found in normal haemopoietic cells, it may be postulated that CD87 can be used alone (when overexpressed) or in combination with CD34 for the detection of minimal residual disease. Results also indicated that patients with UPA

  11. Soluble Urokinase-Type Plasminogen Activator Receptor Plasma Concentration May Predict Susceptibility to High Altitude Pulmonary Edema

    Directory of Open Access Journals (Sweden)

    Matthias Peter Hilty

    2016-01-01

    Full Text Available Introduction. Acute exposure to high altitude induces inflammation. However, the relationship between inflammation and high altitude related illness such as high altitude pulmonary edema (HAPE and acute mountain sickness (AMS is poorly understood. We tested if soluble urokinase-type plasminogen activator receptor (suPAR plasma concentration, a prognostic factor for cardiovascular disease and marker for low grade activation of leukocytes, will predict susceptibility to HAPE and AMS. Methods. 41 healthy mountaineers were examined at sea level (SL, 446 m and 24 h after rapid ascent to 4559 m (HA. 24/41 subjects had a history of HAPE and were thus considered HAPE-susceptible (HAPE-s. Out of the latter, 10/24 HAPE-s subjects were randomly chosen to suppress the inflammatory cascade with dexamethasone 8 mg bid 24 h prior to ascent. Results. Acute hypoxic exposure led to an acute inflammatory reaction represented by an increase in suPAR (1.9±0.4 at SL versus 2.3±0.5 at HA, p<0.01, CRP (0.7±0.5 at SL versus 3.6±4.6 at HA, p<0.01, and IL-6 (0.8±0.4 at SL versus 3.3±4.9 at HA, p<0.01 in all subjects except those receiving dexamethasone. The ascent associated decrease in PaO2 correlated with the increase in IL-6 (r=0.46, p<0.001, but not suPAR (r=0.27, p=0.08; the increase in IL-6 was not correlated with suPAR (r=0.16, p=0.24. Baseline suPAR plasma concentration was higher in the HAPE-s group (2.0±0.4 versus 1.8±0.4, p=0.04; no difference was found for CRP and IL-6 and for subjects developing AMS. Conclusion. High altitude exposure leads to an increase in suPAR plasma concentration, with the missing correlation between suPAR and IL-6 suggesting a cytokine independent, leukocyte mediated mechanism of low grade inflammation. The correlation between IL-6 and PaO2 suggests a direct effect of hypoxia, which is not the case for suPAR. However, suPAR plasma concentration measured before hypoxic exposure may predict HAPE susceptibility.

  12. Tumour microenvironments induce expression of urokinase plasminogen activator receptor (uPAR and concomitant activation of gelatinolytic enzymes.

    Directory of Open Access Journals (Sweden)

    Synnøve Magnussen

    Full Text Available The urokinase plasminogen activator receptor (uPAR is associated with poor prognosis in oral squamous cell carcinoma (OSCC, and increased expression of uPAR is often found at the invasive tumour front. The aim of the current study was to elucidate the role of uPAR in invasion and metastasis of OSCC, and the effects of various tumour microenvironments in these processes. Furthermore, we wanted to study whether the cells' expression level of uPAR affected the activity of gelatinolytic enzymes.The Plaur gene was both overexpressed and knocked-down in the murine OSCC cell line AT84. Tongue and skin tumours were established in syngeneic mice, and cells were also studied in an ex vivo leiomyoma invasion model. Soluble factors derived from leiomyoma tissue, as well as purified extracellular matrix (ECM proteins, were assessed for their ability to affect uPAR expression, glycosylation and cleavage. Activity of gelatinolytic enzymes in the tissues were assessed by in situ zymography.We found that increased levels of uPAR did not induce tumour invasion or metastasis. However, cells expressing low endogenous levels of uPAR in vitro up-regulated uPAR expression both in tongue, skin and leiomyoma tissue. Various ECM proteins had no effect on uPAR expression, while soluble factors originating from the leiomyoma tissue increased both the expression and glycosylation of uPAR, and possibly also affected the proteolytic processing of uPAR. Tumours with high levels of uPAR, as well as cells invading leiomyoma tissue with up-regulated uPAR expression, all displayed enhanced activity of gelatinolytic enzymes.Although high levels of uPAR are not sufficient to induce invasion and metastasis, the activity of gelatinolytic enzymes was increased. Furthermore, several tumour microenvironments have the capacity to induce up-regulation of uPAR expression, and soluble factors in the tumour microenvironment may have an important role in the regulation of posttranslational

  13. Alberta oil sands royalty regime

    International Nuclear Information System (INIS)

    The long term objective of the Oil Sands Business Unit of Alberta Energy is to pave the way for Alberta's bitumen production to reach 3 million barrels per day by 2020. This presentation described the national government's role in resource development. It was emphasized that since the Crown is the owner of the oil sands resource, it would benefit by providing strategic leadership and by generating a larger royalty base. The oil sands fiscal regime was described with reference to generic royalty, risk sharing, investment, and project economics. Business rule principles were also outlined along with criteria for project expansions. Both upstream and downstream challenges and opportunities were listed. 4 figs

  14. Accommodating human values in the climate regime

    NARCIS (Netherlands)

    Cook, Rosalind; Tauschinsky, Eljalill

    2008-01-01

    The climate regime addresses one of the most important challenges facing humankind today. However, while the environmental and economic sides of the problem are well represented, it lacks the inclusion of social and human aspects. The human rights regime, in contrast, is a regime which has been esta

  15. Adaptation in Collaborative Governance Regimes

    Science.gov (United States)

    Emerson, Kirk; Gerlak, Andrea K.

    2014-10-01

    Adaptation and the adaptive capacity of human and environmental systems have been of central concern to natural and social science scholars, many of whom characterize and promote the need for collaborative cross-boundary systems that are seen as flexible and adaptive by definition. Researchers who study collaborative governance systems in the public administration, planning and policy literature have paid less attention to adaptive capacity specifically and institutional adaptation in general. This paper bridges the two literatures and finds four common dimensions of capacity, including structural arrangements, leadership, knowledge and learning, and resources. In this paper, we focus on institutional adaptation in the context of collaborative governance regimes and try to clarify and distinguish collaborative capacity from adaptive capacity and their contributions to adaptive action. We posit further that collaborative capacities generate associated adaptive capacities thereby enabling institutional adaptation within collaborative governance regimes. We develop these distinctions and linkages between collaborative and adaptive capacities with the help of an illustrative case study in watershed management within the National Estuary Program.

  16. Measuring the effectiveness of international environmental regimes

    Energy Technology Data Exchange (ETDEWEB)

    Helm, C.; Sprinz, D.F.

    1999-05-01

    While past research has emphasized the importance of international regimes for international governance, systematic assessments of regime effects are missing. This article derives a standardized measurement concept for the effectiveness of international environmental regimes by developing an operational rational choice calculus to evaluate actual policy simultaneously against a non-regime counterfactual and a collective optimum. Subsequently, the empirical feasibility of the measurement instrument is demonstrated by way of two international treaties regulating transboundary air pollution in Europe. The results demonstrate that the regimes indeed show positive effects - but fall substantially short of the collective optima. (orig.)

  17. Presence of urokinase plasminogen activator, its inhibitor and receptor in small cell lung cancer and non-small cell lung cancer

    DEFF Research Database (Denmark)

    Pappot, H.; Pfeiffer, P.; Grøndahl Hansen, J.;

    1997-01-01

    Spreading of cancer cells is dependent on the combined action of several proteolytic enzymes, such as serine proteases, comprising the urokinase pathway of plasminogen activation. Previous studies of lung cancer indicate that expression, localization and prognostic impact of the components of the...... plasminogen activation system differ in the different non-small cell lung cancer (NSCLC) types, whereas the expression of the components in small cell lung cancer (SCLC) has only sparingly been investigated. In the present study we investigate the presence of the components of the plasminogen activation...... the clinical parameters. This is the first report of a study using a quantitative method to compare levels of the components of the plasminogen activation system in tissue extracts from the two major lung cancer groups. The study shows that uPA, PAI-1 and uPAR are present in SCLC-tissue, suggesting...

  18. Distinctive binding modes and inhibitory mechanisms of two peptidic inhibitors of urokinase-type plasminogen activator with isomeric P1 residues

    DEFF Research Database (Denmark)

    Jiang, Longguang; Zhao, Baoyu; Xu, Peng;

    2015-01-01

    Two isomeric piperidine derivatives (meta and para isomers) were used as arginine mimics in the P1 position of a cyclic peptidic inhibitor (CPAYSRYLDC) of urokinase-type plasminogen activator. The two resulting cyclic peptides showed vastly different affinities (~70 fold) to the target enzyme. X......-ray crystal structure analysis showed that the two P1 residues were inserted into the S1 specificity pocket in indistinguishable manners. However, the rest of the peptides bound in entirely different ways on the surface of the enzyme, and the two peptides have different conformations, despite the highly...... similar sequence. These results demonstrate how the subtle difference in P1 residue can dictate the exosite interactions and the potencies of peptidic inhibitors, and highlight the importance of P1 residue for protease inhibition. This study provides important information for the development of peptidic...

  19. Elevated soluble urokinase receptor values in CSF, age and bacterial meningitis infection are independent and additive risk factors of fatal outcome

    DEFF Research Database (Denmark)

    Tzanakaki, G; Paparoupa, M; Kyprianou, M;

    2012-01-01

    The aim of the present study was to evaluate the potential role of cerebrospinal fluid soluble urokinase receptor (suPAR) level, infection and age as risk factors for fatal outcome in patients suspected of having meningitis and/or bacteraemia on admission to hospital. A total of 545 cerebrospinal...... fluid samples from patients with clinically suspected meningitis were sent to the Hellenic National Meningitis Reference Laboratory. Ten of 545 (1.83%) patients died. Analysis by receiver operating characteristics (ROC) curve revealed that both suPAR and age were significant for prediction of fatal...... outcome. Patients with levels of suPAR above the cut-off values and age ≥51 years, or patients in which either Neisseria meningitis or Streptococcus pneumoniae were detected were categorized as high risk patients. The combination of the above three predictors (suPAR, age and infectious agent...

  20. Levels of plasminogen activator inhibitor type 1 and urokinase plasminogen activator receptor in non-small cell lung cancer as measured by quantitative ELISA and semiquantitative immunohistochemistry

    DEFF Research Database (Denmark)

    Pappot, Helle; Skov, Birgit Guldhammer; Pyke, Charles;

    1997-01-01

    , results in non-small cell lung cancer (NSCLC) studies obtained by quantitative ELISA and semiquantitative immunohistochemistry differ. If the prognostic value of the components of the plasminogen activation system is to be exploited clinically in the future, it is important to choose an easy and valid......PAR with the aim of predicting prognosis. In conclusion, a larger comparative study is needed to clarify the relationship between ELISA and immunohistochemical results, before a methodology for clinical use can be chosen in non-small cell lung cancer....... methodology. In the present study we investigated levels of plasminogen activator inhibitor type 1 (PAI-I) and urokinase plasminogen activator receptor (uPAR), as quantitated by ELISA in tumour extracts from 64 NSCLC patients (38 squamous cell carcinomas, 26 adenocarcinomas), and compared them to staining...

  1. Soluble urokinase plasminogen activator receptor (suPAR) is a novel, independent predictive marker of myocardial infarction in HIV-1-infected patients

    DEFF Research Database (Denmark)

    Rasmussen, Line Jee Hartmann; Knudsen, A; Katzenstein, T L;

    2016-01-01

    OBJECTIVES: Patients infected with HIV are at increased risk of myocardial infarction (MI). Increased plasma levels of the inflammatory biomarker soluble urokinase plasminogen activator receptor (suPAR) have been associated with increased risk of cardiovascular diseases (CVD), including MI in the......, gender, duration of antiretroviral therapy (ART), smoking and no known CVD. suPAR was measured in the four plasma samples available for each patient at different time-points; 1, Before initiation of ART; 2, 3 months after initiation of ART; 3, 1 year before the case's MI; and 4, The last sample available...... before the case's MI. RESULTS: In unadjusted conditional regression analysis, higher levels of suPAR were associated with a significant increase in risk of MI at all time-points. Patients in the third and fourth suPAR quartiles had a three- to 10-fold higher risk of MI compared to patients in the lowest...

  2. Plasma soluble urokinase-type plasminogen activator receptor level is independently associated with coronary microvascular function in patients with non-obstructive coronary artery disease

    DEFF Research Database (Denmark)

    Mekonnen, Girum; Corban, Michel T; Hung, Olivia Y;

    2015-01-01

    BACKGROUND: Soluble urokinase-type plasminogen activator receptor (suPAR) is a novel biomarker released from leukocytes and endothelial cells that has been associated with atherosclerotic cardiovascular disease. We hypothesized that plasma suPAR level is an independent predictor of coronary...... microvascular function. METHODS: Coronary blood flow velocity and plasma suPAR levels were evaluated in patients with non-obstructive coronary artery disease. Coronary flow reserve (CFR) was calculated as the ratio of hyperemic to basal average peak blood flow velocity and coronary microvascular dysfunction was...... defined as CFR ≤ 2.0 in the setting of a fractional flow reserve value of ≥0.75. Plasma suPAR levels were measured using ELISA technique. The association between suPAR and CFR was investigated using univariate and multivariate regression analyses. RESULTS: In 66 patients, 47% were men, 26% had diabetes...

  3. Gene expression of fibrinolytic factors urokinase plasminogen activator and plasminogen activator inhibitor-1 in rabbit temporo-mandibular joint cartilage with disc displacement

    Institute of Scientific and Technical Information of China (English)

    ZHAN Jing; GU Zhi-yuan; WU Li-qun; ZHANG Yin-kai; HU Ji-an

    2005-01-01

    Background The urokinase plasminogen activator system is believed to play an important role in degradation of the extracellular matrix associated with cartilage and bone destruction; however its precise roles in temporomandibular disorders have not yet been clarified. The aims of this study were to investigate the gene expression of fibrinolytic factors urokinase plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) in the articular cartilage of rabbit temporomandibular joint (TMJ) with disc displacement (DD) and to probe the relationship between fibrinolytic activity and cartilage remodeling. Methods Disc displacement of right joints was performed in 36 of 78 rabbits under investigation. The animals were sacrificed at 4 days and 1, 2, 4, 8 and 12 weeks after surgery, respectively. The right joints of these animals were harvested and processed for the examination of mRNA expression of uPA and PAI-1 in articular cartilage using in situ hybridization techniques. Results The expression of uPA and PAI-1 was co-expressed weakly in the chondrocytes from transitive zone to hypertrophic zone and mineralized zone, while no hybridizing signals were shown in proliferative zone and superficial zone in control rabbits. The most striking was the up-regulation of uPA and PAI-1 mRNA in 4-day rabbits postoperatively at the onset of cartilage degeneration. The strongest hybridizing signals for uPA and PAI-1 were seen in 2-week rabbits postoperatively. After 2 weeks, the expression of uPA and PAI-1 began to decrease and reached nearly normal level at 12 weeks. Conclusions The expression of the uPA/PAI-1 system coincides with the pathological changes in condylar cartilage after DD. The uPA/PAI-1 system may be one of the essential mediators in articular cartilage remodeling.

  4. Transcriptional Regulation of Urokinase-type Plasminogen Activator Receptor by Hypoxia-Inducible Factor 1 Is Crucial for Invasion of Pancreatic and Liver Cancer

    Directory of Open Access Journals (Sweden)

    Peter Büchler

    2009-02-01

    Full Text Available Angioinvasion is critical for metastasis with urokinase-type plasminogen activator receptor (uPAR and tumor hypoxia-activated hypoxia-inducible factor 1 (HIF-1 as key players. Transcriptional control of uPAR expression by HIF has never been reported. The aim of the present study, therefore, was to test whether tumor hypoxia-induced HIF expression may be linked to transcriptional activation of uPAR and dependent angioinvasion. We used human pancreatic cancer cells and a model of parental and derived HIF-1β-deficient mouse liver cancer cell lines and performed Northern blot analysis, nuclear runoff assays, electrophoretic mobility shift assay, polymerase chain reaction-generated deletion mutants, luciferase assays, Matrigel invasion assays, and in vivo angioinvasion assays in the chorioallantoic membrane of fertilized chicken eggs. Urokinase-type plasminogen activator receptor promoter analysis resulted in four putative HIF binding sites. Hypoxia strongly induced de novo transcription of uPAR mRNA. With sequential deletion mutants of the uPAR promoter, it was possible to identify one HIF binding site causing a nearly 200-fold increase in luciferase activity. Hypoxia enhanced the number of invading tumor cells in vitro and in vivo. In contrast, HIF-1β-deficient cells failed to upregulate uPAR expression, to activate luciferase activity, and to invade on hypoxia. Taken together, we show for the first time that uPAR is under transcriptional control of HIF and that this is important for hypoxia-induced metastasis.

  5. Urokinase-type Plasminogen Activator-like Proteases in Teleosts Lack Genuine Receptor-binding Epidermal Growth Factor-like Domains*

    Science.gov (United States)

    Bager, René; Kristensen, Thomas K.; Jensen, Jan K.; Szczur, Agnieszka; Christensen, Anni; Andersen, Lisbeth M.; Johansen, Jesper S.; Larsen, Niels; Baatrup, Erik; Huang, Mingdong; Ploug, Michael; Andreasen, Peter A.

    2012-01-01

    Plasminogen activation catalyzed by urokinase-type plasminogen activator (uPA) plays an important role in normal and pathological tissue remodeling processes. Since its discovery in the mid-1980s, the cell membrane-anchored urokinase-type plasminogen activator receptor (uPAR) has been believed to be central to the functions of uPA, as uPA-catalyzed plasminogen activation activity appeared to be confined to cell surfaces through the binding of uPA to uPAR. However, a functional uPAR has so far only been identified in mammals. We have now cloned, recombinantly produced, and characterized two zebrafish proteases, zfuPA-a and zfuPA-b, which by several criteria are the fish orthologs of mammalian uPA. Thus, both proteases catalyze the activation of fish plasminogen efficiently and both proteases are inhibited rapidly by plasminogen activator inhibitor-1 (PAI-1). But zfuPA-a differs from mammalian uPA by lacking the exon encoding the uPAR-binding epidermal growth factor-like domain; zfuPA-b differs from mammalian uPA by lacking two cysteines of the epidermal growth factor-like domain and a uPAR-binding sequence comparable with that found in mammalian uPA. Accordingly, no zfuPA-b binding activity could be found in fish white blood cells or fish cell lines. We therefore propose that the current consensus of uPA-catalyzed plasminogen activation taking place on cell surfaces, derived from observations with mammals, is too narrow. Fish uPAs appear incapable of receptor binding in the manner known from mammals and uPA-catalyzed plasminogen activation in fish may occur mainly in solution. Studies with nonmammalian vertebrate species are needed to obtain a comprehensive understanding of the mechanism of plasminogen activation. PMID:22733817

  6. Bacterial endotoxin enhances colorectal cancer cell adhesion and invasion through TLR-4 and NF-kappaB-dependent activation of the urokinase plasminogen activator system.

    LENUS (Irish Health Repository)

    Killeen, S D

    2009-05-19

    Perioperative exposure to lipopolysaccharide (LPS) is associated with accelerated metastatic colorectal tumour growth. LPS directly affects cells through Toll-like receptor 4 (TLR-4) and the transcription factor NF-kappaB. The urokinase plasminogen activator (u-PA) system is intimately implicated in tumour cell extracellular matrix (ECM) interactions fundamental to tumour progression. Thus we sought to determine if LPS directly induces accelerated tumour cell ECM adhesion and invasion through activation of the u-PA system and to elucidate the cellular pathways involved. Human colorectal tumour cell lines were stimulated with LPS. u-PA concentration, u-PA activity, active u-PA, surface urokinase plasminogen activator receptor (u-PAR) and TLR-4 expression were assessed by ELISA, colorimetric assay, western blot analysis and flow cytometry respectively. In vitro tumour cell vitronectin adhesion and ECM invasion were analysed by vitronectin adhesion assay and ECM invasion chambers. u-PA and u-PAR function was inhibited with anti u-PA antibodies or the selective u-PA inhibitors amiloride or WXC-340, TLR-4 by TLR-4-blocking antibodies and NF-kappaB by the selective NF-kappaB inhibitor SN-50. LPS upregulates u-PA and u-PAR in a dose-dependent manner, enhancing in vitro tumour cell vitronectin adhesion and ECM invasion by >40% (P<0.01). These effects were ameliorated by u-PA and u-PAR inhibition. LPS activates NF-kappaB through TLR-4. TLR-4 and NF-kappaB inhibition ameliorated LPS-enhanced u-PA and u-PAR expression, tumour cell vitronectin adhesion and ECM invasion. LPS promotes tumour cell ECM adhesion and invasion through activation of the u-PA system in a TLR-4- and NF-kappaB-dependent manner.

  7. Mapping the topographic epitope landscape on the urokinase plasminogen activator receptor (uPAR by surface plasmon resonance and X-ray crystallography

    Directory of Open Access Journals (Sweden)

    Baoyu Zhao

    2015-12-01

    Full Text Available The urokinase-type plasminogen activator receptor (uPAR or CD87 is a glycolipid-anchored membrane protein often expressed in the microenvironment of invasive solid cancers and high levels are generally associated with poor patient prognosis (Kriegbaum et al., 2011 [1]. uPAR is organized as a dynamic modular protein structure composed of three homologous Ly6/uPAR domains (LU.This internally flexible protein structure of uPAR enables an allosteric regulation of the interactions with its two principal ligands: the serine protease urokinase-type plasminogen activator (uPA and the provisional matrix protein vitronectin (Vn (Mertens et al., 2012; Gårdsvoll et al., 2011; Madsen et al., 2007 [2–4]. The data presented here relates to the non-covalent trapping of one of these biologically relevant uPAR-conformations by a novel class of monoclonal antibodies (Zhao et al., 2015 [5] and to the general mapping of the topographic epitope landscape on uPAR. The methods required to achieve these data include: (1 recombinant expression and purification of a uPAR-hybrid protein trapped in the desired conformation [patent; WO 2013/020898 A12013]; (2 developing monoclonal antibodies with unique specificities using this protein as antigen; (3 mapping the functional epitope on uPAR for these mAbs by surface plasmon resonance with a complete library of purified single-site uPAR mutants (Zhao et al., 2015; Gårdsvoll et al., 2006 [5,6]; and finally (4 solving the three-dimensional structures for one of these mAbs by X-ray crystallography alone and in complex with uPAR [deposited in the PDB database as 4QTH and 4QTI, respectively].

  8. Mapping the topographic epitope landscape on the urokinase plasminogen activator receptor (uPAR) by surface plasmon resonance and X-ray crystallography.

    Science.gov (United States)

    Zhao, Baoyu; Gandhi, Sonu; Yuan, Cai; Luo, Zhipu; Li, Rui; Gårdsvoll, Henrik; de Lorenzi, Valentina; Sidenius, Nicolai; Huang, Mingdong; Ploug, Michael

    2015-12-01

    The urokinase-type plasminogen activator receptor (uPAR or CD87) is a glycolipid-anchored membrane protein often expressed in the microenvironment of invasive solid cancers and high levels are generally associated with poor patient prognosis (Kriegbaum et al., 2011 [1]). uPAR is organized as a dynamic modular protein structure composed of three homologous Ly6/uPAR domains (LU).This internally flexible protein structure of uPAR enables an allosteric regulation of the interactions with its two principal ligands: the serine protease urokinase-type plasminogen activator (uPA) and the provisional matrix protein vitronectin (Vn) (Mertens et al., 2012; Gårdsvoll et al., 2011; Madsen et al., 2007 [2-4]). The data presented here relates to the non-covalent trapping of one of these biologically relevant uPAR-conformations by a novel class of monoclonal antibodies (Zhao et al., 2015 [5]) and to the general mapping of the topographic epitope landscape on uPAR. The methods required to achieve these data include: (1) recombinant expression and purification of a uPAR-hybrid protein trapped in the desired conformation [patent; WO 2013/020898 A12013]; (2) developing monoclonal antibodies with unique specificities using this protein as antigen; (3) mapping the functional epitope on uPAR for these mAbs by surface plasmon resonance with a complete library of purified single-site uPAR mutants (Zhao et al., 2015; Gårdsvoll et al., 2006 [5,6]); and finally (4) solving the three-dimensional structures for one of these mAbs by X-ray crystallography alone and in complex with uPAR [deposited in the PDB database as 4QTH and 4QTI, respectively]. PMID:26504891

  9. Regime Jumps in Electricity Prices

    International Nuclear Information System (INIS)

    Electricity prices are known to be very volatile and subject to frequent jumps due to system breakdown, demand shocks, and inelastic supply. As many international electricity markets are in some state of deregulation, more and more participants in these markets are exposed to these stylised facts. Appropriate pricing, portfolio, and risk management models should incorporate these facts. Authors have introduced stochastic jump processes to deal with the jumps, but we argue and show that this specification might lead to problems with identifying the true mean-reversion within the process. Instead, we propose using a regime jump model that disentangles mean-reversion from jump behaviour. This model resembles more closely the true price path of electricity prices

  10. Propagation Regime of Iron Dust Flames

    Science.gov (United States)

    Tang, Francois-David; Goroshin, Samuel; Higgins, Andrew J.

    2012-01-01

    A flame propagating through an iron-dust mixture can propagate in two asymptotic regimes. When the characteristic time of heat transfer between particles is much smaller than the characteristic time of particle combustion, the flame propagates in the continuum regime where the heat released by reacting particles can be modelled as a space-averaged function. In contrast, when the characteristic time of heat transfer is much larger than the particle reaction time, the flame can no longer be treated as a continuum due to dominating effects associated with the discrete nature of the particle reaction. The discrete regime is characterized by weak dependence of the flame speed on the oxygen concentration compared to the continuum regime. The discrete regime is observed in flames propagating through an iron dust cloud within a gas mixture containing xenon, while the continuum regime is obtained when xenon is substituted with helium.

  11. Accommodating human values in the climate regime

    Directory of Open Access Journals (Sweden)

    Rosalind Cook

    2008-12-01

    Full Text Available The climate regime addresses one of the most important challenges facing humankind today. However, while the environmental and economic sides of the problem are well represented, it lacks the inclusion of social and human aspects. The human rights regime, in contrast, is a regime which has been established precisely to implement human values. This article ex-plains the problems of climate change in terms of human values and argues that some proce-dures from the human rights regime offer possibilities for improvement. It is submitted that through the inclusion of human rights instruments, such as individual communication, pro-gressive realisation and authoritative interpretation, the inclusion of human values into the climate regime will be facilitated. This article presents these instruments and discusses their potential for inclusion in the climate regime.

  12. Commodity Price Volatility Across Exchange Rate Regimes

    OpenAIRE

    John T. Cuddington; Hong Liang

    1998-01-01

    This paper documents a new "stylized fact" regarding commodity prices using alternative datasets covering the period from 1880 to 1996: The volatility of real commodity prices, defined as nominal commodity prices deflated by the manufacturing unit value index, is higher under flexible-exchange rate regimes than fixed-exchange rate regimes. Furthermore, changes in exchange regime are associated with changes in the persistence of commodity price shocks. Implications of this finding for open-eco...

  13. US Forest Service LANDFIRE Historical Fire Regimes

    Data.gov (United States)

    US Forest Service, Department of Agriculture — Historical fire regimes, intervals, and vegetation conditions are mapped using the Vegetation Dynamics Development Tool (VDDT). These data support fire and...

  14. Synergies between nonproliferation regimes: A pragmatic approach

    International Nuclear Information System (INIS)

    Full text: With the recent progress in establishing international nonproliferation regimes, the question of synergies between different verification and monitoring regimes is becoming more acute. Three multilateral and universal nonproliferation organisations covering safeguards on civil nuclear materials, nuclear testing, and chemical weapons are up and running. A regime on biological weapons is under negotiation. Several regional organisations concerned with monitoring nonproliferation commitments in the nuclear field are in place; others are being established. Past discussions on synergies between these regimes have suffered from being too far-reaching. These discussions often have not reflected adequately the political difficulties of cooperation between regimes with different membership, scope and institutional set-up. This paper takes a pragmatic look at exploiting synergies and identifies some potential and real overlaps in the work between different verification regimes. It argues for a bottom-up approach and identifies building blocks for collaboration between verification regimes. By realising such, more limited potential for cooperation, the ground could be prepared for exploiting other synergies between these regimes. (author)

  15. Capacitance densitometer for flow regime identification

    Science.gov (United States)

    Shipp, Jr., Roy L.

    1978-01-01

    This invention relates to a capacitance densitometer for determining the flow regime of a two-phase flow system. A two-element capacitance densitometer is used in conjunction with a conventional single-beam gamma densitometer to unambiguously identify the prevailing flow regime and the average density of a flowing fluid.

  16. LEGAL MATRIMONIAL REGIME IN B&H

    Directory of Open Access Journals (Sweden)

    Boris Krešić

    2011-12-01

    Full Text Available Matrimonial regime between spouses or between extramarital partners, and between parents and children is regulated by the Family Law Act of Bosnia and Herzegovina Federation, hereinafter FLA B&HF (SG FBiH 35/05, 41/05, Family Law Act of the Republic of Srpska, hereinafter FLA RS (SG RS”54/02, 41/08 and the Family Law Act of Brčko District, hereinafter FLA BD (SG RS, 66/07. Legal rules used for the regulation of the matrimonial regime between spouses, as well as between spouses and third parties make matrimonial regime (Ponjavić, 2005, p. 361. Matrimonial regime between spouses in family legislation in Bosnia and Herzegovina (B&H is regulated in two following ways: as legal matrimonial regime and as contract matrimonial regime. Legal regime is the one which applies on spouses if not arranged otherwise prior to contracting marriage or during marriage. In this paper the author indicates the differences between the legal matrimonial regimes of the two entities as well as those between the entities and Brčko District of Bosnia and Herzegovina.

  17. Explaining the Transition Between Exchange Rate Regimes

    OpenAIRE

    Masson, Paul; RUGE-MURCIA, Francisco J.

    2003-01-01

    This paper studies the transition between exchange rate regimes using a Markov chain model with time-varying transition probabilities. The probabilities are parameterized as nonlinear functions of variables suggested by the currency crisis and optimal currency area literature. Results using annual data indicate that inflation, and to a lesser extent, output growth and trade openness help explain the exchange rate regime transition dynamics.

  18. Multiscale regime shifts and planetary boundaries

    NARCIS (Netherlands)

    Hughes, T.P.; Carpenter, S.; Rockstrom, J.; Scheffer, M.; Walker, B.

    2013-01-01

    Life on Earth has repeatedly displayed abrupt and massive changes in the past, and there is no reason to expect that comparable planetary-scale regime shifts will not continue in the future. Different lines of evidence indicate that regime shifts occur when the climate or biosphere transgresses a ti

  19. LOW DOSE MAGNESIUM SULPHATE REGIME FOR ECLAMPSIA

    Directory of Open Access Journals (Sweden)

    Bangal V

    2009-09-01

    Full Text Available Pre- eclampsia is one of the commonest medical complications seen during pregnancy. It contributes significantly to maternal and perinatal morbidity and mortality. Dr.J.A.Pritchard in 1955, introduced magnesium sulphate for control of convulsions in eclampsia and is used worldwide. Considering the low body mass index of indian women, a low dose magnesium sulphate regime has been introduced by some authors. Present study was carried out at tertiary care centre in rural area. Fifty cases of eclampsia were randomly selected to find out the efficacy of low dose magnesium sulphate regime to control eclamptic convulsions. Maternal and perinatal outcome and magnesium toxicity were analyzed. It was observed that 86% cases responded to initial intravenous dose of 4 grams of 20% magnesium sulphate . Eight percent cases, who got recurrence of convulsion, were controlled by additional 2 grams of 20% magnesium sulphate. Six percent cases required shifting to standard Pritchard regime, as they did not respond to low dose magnesium sulphate regime. The average total dose of magnesium sulphate required for control of convulsions was 20 grams ie. 54.4% less than that of standard Pritchard regime. The maternal and perinatal morbidity and mortality in the present study werecomparable to those of standard Pritchard regime. The study did not find a single case of magnesium related toxicity with low dose magnesium sulphate regime. Low dose magnesium sulphate regime was found to be safe and effective in eclampsia.

  20. Framing of regimes and transition strategies

    DEFF Research Database (Denmark)

    Jensen, Jens Stissing

    2012-01-01

    This article suggests that transition strategies are always formulated in the context of specific representations of the regime and the challenges it faces. It is argued that the framing of a regime affects the envisioning of transition strategies. An analysis of the current development agenda...

  1. Food Regimes Revisited: A New Zealand Perspective

    Directory of Open Access Journals (Sweden)

    Michael Roche

    2012-01-01

    Full Text Available ‘Food Regimes’ was coined by Friedmann and McMichael in 1989 and provided a organising framework for a considerable amount of Australian and New Zealand research during a period of economic restructuring and ‘deregulation’. Subsequently Food regimes were overtaken by other perspectives in New Zealand and elsewhere including an interest in commodity productions chains, regulation, post-productivist landscapes, and post structural political economy. More recently McMichael has reintroduced Food Regimes to his analysis. The paper will compare and contrast McMichael’s earlier and more recent engagements with Food Regimes. The export meat, the dairy, and pip fruit industries of Zealand it will be used to illustrate points about the timing, boundaries and margins as well as the transformation of Food Regimes. Finally the paper will attempt to connect the more recent focus on commodity chain analysis and post productivist landscapes with McMichael’s renewed interest in food Regimes.

  2. Signalling networks associated with urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 in breast cancer tissues: new insights from protein microarray analysis.

    Science.gov (United States)

    Wolff, Claudia; Malinowsky, Katharina; Berg, Daniela; Schragner, Kerstin; Schuster, Tibor; Walch, Axel; Bronger, Holger; Höfler, Heinz; Becker, Karl-Friedrich

    2011-01-01

    The urokinase-type plasminogen activator (uPA) and the main uPA inhibitor PAI-1 play important roles in cell migration and invasion in both physiological and pathological contexts. Both factors are clinically applicable predictive markers in node-negative breast cancer patients that are used to stratify patients for adjuvant chemotherapy. In addition to their classical functions in plasmin regulation, both factors are key components in cancer-related cell signalling. Such signalling cascades are well described in cell culture systems, but a better understanding of uPA- and PAI-1-associated signalling networks in clinical tissues is needed. We examined the expression of uPA, PAI-1, and 21 signalling molecules in 201 primary breast cancer tissues using protein microarrays. Expression of uPA was significantly correlated with the expression of ERK and Stat3, while expression of PAI-1 was correlated with the uPA receptor and Akt activation, presumably via integrin and HER-receptor signalling. Analysis of uPA expression did not reveal any significant correlation with staging, grading or age of the patients. The PAI-1 expression was correlated with nodal stage. Network monitoring for uPA and PAI-1 in breast cancer reveals interactions with main signalling cascades and extends the findings from cell culture experiments. Our results reveal possible mechanisms underlying cancer development.

  3. TISSUE INHIBITOR OF METALLOPROTEINASE 1, MATRIX METALLOPROTEINASE 9, ALPHA-1 ANTITRYPSIN, METALLOTHIONEIN AND UROKINASE TYPE PLASMINOGEN ACTIVATOR RECEPTOR IN SKIN BIOPSIES FROM PATIENTS AFFECTED BY AUTOIMMUNE BLISTERING DISEASES

    Directory of Open Access Journals (Sweden)

    Ana Maria Abreu Velez

    2013-07-01

    Full Text Available Introduction: Proteinases and proteinase inhibitors have been described to play a role in autoimmune skin blistering diseases. We studied skin lesional biopsies from patients affected by several autoimmune skin blistering diseases for proteinases and proteinase inhibitors. Methods: We utilized immunohistochemistry to evaluate biopsies for alpha-1-antitrypsin, human matrix metalloproteinase 9 (MMP9, human tissue inhibitor of metalloproteinases 1 (TIMP-1, metallothionein and urokinase type plasminogen activator receptor (uPAR. We tested 30 patients affected by endemic pemphigus, 30 controls from the endemic area, and 15 normal controls. We also tested 30 biopsies from patients with bullous pemphigoid (BP, 20 with pemphigus vulgaris (PV, 8 with pemphigus foliaceus, and 14 with dermatitis herpetiformis (DH. Results: Contrary to findings in the current literature, most autoimmune skin blistering disease biopsies were negative for uPAR and MMP9. Only some chronic patients with El Bagre-EPF were positive to MMP9 in the dermis, in proximity to telocytes. TIMP-1 and metallothionein were positive in half of the biopsies from BP patients at the basement membrane of the skin, within several skin appendices, in areas of dermal blood vessel inflammation and within dermal mesenchymal-epithelial cell junctions.

  4. The diagnostic value of soluble urokinase plasminogen activator receptor compared with C-reactive protein and procalcitonin in children with febrile neutropenia.

    Science.gov (United States)

    Sirinoglu, Melis; Soysal, Ahmet; Karaaslan, Ayşe; Kepenekli Kadayifci, Eda; Cinel, Ismail; Koç, Ahmet; Tokuç, Gülnur; Yaman, Ali; Haklar, Goncagül; Şirikçi, Önder; Turan, Serap; Altınkanat Gelmez, Gülşen; Söyletir, Güner; Bakır, Mustafa

    2016-04-01

    The aim of the present study was to determine the diagnostic value of soluble urokinase plasminogen activator receptor (suPAR) in pediatric patients with febrile neutropenia. A prospective case-control study was performed. Patients included 29 children with febrile neutropenia (FN) and 27 control subjects without any infection or immunosuppressive condition. Blood samples were obtained on the day of admission and on the 4th to 7th days of the hospital stay. The median (minimum-maximum) serum levels of suPAR obtained on the first day of the admission were 2.08 (0.93-9.42) and 2.22 (1.08-5.13) ng/mL for the FN group and the control group, respectively. The median serum levels of suPAR in the FN and control groups were not significantly different (P = .053). The mean serum suPAR level was significantly higher in nonsurvivors than in survivors in the FN group (P < .05). In the FN group, the area under the receiver operating characteristics curve (AUCROC) for suPAR was 0.546, but no optimum cutoff value, sensitivity, specificity, negative predictive value (NPV), or positive predictive value (PPV) was obtained. We conclude that suPAR is not useful as a diagnostic biomarker in children with febrile neutropenia; however, persistent high serum suPAR level may predict mortality in FN in children. PMID:27057782

  5. Short-Term Catheter-Directed Thrombolysis with Low-Dose Urokinase Followed by Aspiration Thrombectomy for Treatment of Symptomatic Lower Extremity Deep Venous Thrombosis

    Energy Technology Data Exchange (ETDEWEB)

    Jung, Se Hee; Lim, Nam Yeul; Song, Jang Hyeon [Dept. of Radiology, Chonnam National University Hospital, Gwangju (Korea, Republic of); Kim, Jae Kyu; Lim, Jae Hoon [Dept. of Radiology, Hospital, Ulsan University School of Medicine, Gweangju (Korea, Republic of); Chang, Nam Kyu [Dept. of Radiology, Chonnam National University Hwasun Hospital, Hwasun (Korea, Republic of); Choi, Soo Jin Na; Chung, Sang Young [Dept. of Radiology, Chonnam National University Hospital, Chonnam National University School of Medicine, Gwangju (Korea, Republic of)

    2011-10-15

    To evaluate the venous patency in patients treated by catheter-directed thrombolysis with low-dose urokinase (UK) for symptomatic lower extremity deep venous thrombosis (DVT). Eighty-nine consecutive patients (46 women and 43 men; mean age, 58.1 years), treated by catheter-directed thrombolysis with low-dose UK were included in this study. Immediate venous patency was evaluated in terms of technical success (successful restoration of antegrade in-line flow in the treated vein with residual stenosis rate of less than 30%) and clinical success (significant reduction of clinical symptoms before hospital discharge). Late venous patency was evaluated in terms of primary patency rate and clinical success. Immediate technical success was achieved in all patients and immediate clinical success in 80 (90%) patients. There was no major systemic bleeding complication. The primary patency rate at 6 months and 12 months was 84% and 79%, respectively. Fifty-six (63%) patients were asymptomatic after a median clinical follow-up of 18 months, eleven (12%) patients improved moderately, seven (8%) patients remained unchanged, and fifteen (17%) patients had no clinical follow-up. Short-term catheter-directed thrombolysis with low-dose UK can be an effective, safe method to manage DVT of the lower extremities.

  6. The diagnostic value of soluble urokinase plasminogen activator receptor compared with C-reactive protein and procalcitonin in children with febrile neutropenia.

    Science.gov (United States)

    Sirinoglu, Melis; Soysal, Ahmet; Karaaslan, Ayşe; Kepenekli Kadayifci, Eda; Cinel, Ismail; Koç, Ahmet; Tokuç, Gülnur; Yaman, Ali; Haklar, Goncagül; Şirikçi, Önder; Turan, Serap; Altınkanat Gelmez, Gülşen; Söyletir, Güner; Bakır, Mustafa

    2016-04-01

    The aim of the present study was to determine the diagnostic value of soluble urokinase plasminogen activator receptor (suPAR) in pediatric patients with febrile neutropenia. A prospective case-control study was performed. Patients included 29 children with febrile neutropenia (FN) and 27 control subjects without any infection or immunosuppressive condition. Blood samples were obtained on the day of admission and on the 4th to 7th days of the hospital stay. The median (minimum-maximum) serum levels of suPAR obtained on the first day of the admission were 2.08 (0.93-9.42) and 2.22 (1.08-5.13) ng/mL for the FN group and the control group, respectively. The median serum levels of suPAR in the FN and control groups were not significantly different (P = .053). The mean serum suPAR level was significantly higher in nonsurvivors than in survivors in the FN group (P febrile neutropenia; however, persistent high serum suPAR level may predict mortality in FN in children.

  7. Extracellular Matrix Biomarker, Fibulin-1, Is Closely Related to NT-proBNP and Soluble Urokinase Plasminogen Activator Receptor in Patients with Aortic Valve Stenosis (The SEAS Study)

    DEFF Research Database (Denmark)

    Kruger, Ruan; Rasmussen, Lars M; Argraves, William S;

    2014-01-01

    BACKGROUND: Fibulin-1, a circulating extracellular matrix glycoprotein, has been associated with arterial disease and elevated N-terminal prohormone B-type natriuretic peptide (NT-proBNP) in diabetes. Soluble urokinase plasminogen activator receptor (suPAR), a marker of inflammation, has been...... associated with subclinical atherosclerosis. Therefore, we aimed to explore the interplay between these biomarkers and mild to moderate aortic valve stenosis (AS). METHODS: In 374 patients with mild to moderate AS, we investigated the relationship of fibulin-1 with NT-proBNP, levels of suPAR and the degree...... of AS at baseline and after one and four years of treatment with Simvastatin 40 mg and Ezetimibe 10 mg or placebo. RESULTS: During treatment, fibulin-1 became more closely associated with NT-proBNP (βyear0 = 0.10, p = 0.08, βyear1 = 0.16, p = 0.005, βyear4 = 0.22, p

  8. Phorbol ester induces the biosynthesis of glycosylated and nonglycosylated plasminogen activator inhibitor 2 in high excess over urokinase-type plasminogen activator in human U-937 lymphoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Genton, C.; Kruithof, E.K.; Schleuning, W.D.

    1987-03-01

    The tumor-promoting phorbol ester PMA induces changes in the histiocytic human lymphoma cell line U-937 akin to cellular differentiation and concomitantly stimulates the biosynthesis of plasminogen activator inhibitor 2 (PAI 2) and of urokinase-type plasminogen activator (u-PA). PAI 2 is found in a nonglycosylated intracellular and a glycosylated secreted form. The former appears to be identical to PAI 2 previously purified from placental extracts and large-scale U-937 cell cultures. The sixfold increase of PAI 2 antigen measured 24 h after PMA treatment in cell extracts and conditioned media is accompanied by an equal increase of active PAI 2 mRNA, whereas the 6 to 13-fold increase of u-PA antigen in the same samples is associated with only a 1.5-fold mRNA increase. The increase of PAI 2, but not of u-PA, biosynthesis requires transcription. A 50-fold molar excess of PAI 2 over u-PA is found in both extracts and conditioned media of PMA-treated cells. PAI 2 represents at least 0.3% of total de novo synthesized protein 24 h after induction with PMA. Thus, PAI 2, but not u-PA, is an abundant product of this precursor analogue of the mononuclear phagocyte lineage, and might represent a new marker for monocyte/macrophage differentiation.

  9. What is the economy of Ancien Regime?

    OpenAIRE

    Jean-Yves Grenier

    2012-01-01

    The aim of this article is to describe the main features of the Ancien Regime economy and develop a “political economy” of European societies, particularly the French, during the central period of modernity (XVII-XVIII centuries

  10. Earth Regime Network Evolution Study (ERNESt)

    Science.gov (United States)

    Menrad, Bob

    2016-01-01

    Speaker and Presenter at the Lincoln Laboratory Communications Workshop on April 5, 2016 at the Massachusetts Institute of Technology Lincoln Laboratory in Lexington, MA. A visual presentation titled Earth Regimes Network Evolution Study (ERNESt).

  11. FLOW REGIMES BELOW AERATORS FOR DISCHARGE TUNNELS

    Institute of Scientific and Technical Information of China (English)

    MA Fei; WU Jian-hua

    2012-01-01

    The flow regimes below an aerator influence directly the air entrainment and the cavitation damage control.Based on the theoretical considerations,the experiments of the aerator for a discharge tunnel were conducted,and the relationships between the flow regime and hydraulic and geometric parameters were investigated.The results showed that,there are two kinds of threshold values for the flow regime conversions.One is Fr1-2 standing for the conversion from the fully filled cavity to the partially filled cavity,and the other is Fr2-3 which shows the change from the partially filled cavity to the net air cavity.Two empirical expressions were obtained for the conversions of the flow regimes,which can be used in the designs of the aerators.

  12. The Two Regimes of Postwar Shipping

    DEFF Research Database (Denmark)

    Iversen, Martin Jes; Tenold, Stig

    2014-01-01

    the bargaining that accompanied the shift from the national regime to the competitive regime. Specifically, we show that the new regime primarily accommodated the interests of private actors such as shipping companies, rather than the interests of the authorities and the trade unions.......The aim of this article is to illustrate the most important changes in the regulatory framework of the shipping sector from the 1960s to 2010, and to analyse the basis for, and effects of, these changes. In order to explain how the transformation has occurred, we use two traditional maritime...... nations—Denmark and Norway—as case studies. First, we introduce the two regimes of Danish and Norwegian shipping: ‘the national regime’ from the early 1960s to the mid-1970s; and ‘the competitive regime’, which was fully established by the middle of the 1990s and still persists. Then, we briefly sketch...

  13. Regimes of validity for balanced models

    Science.gov (United States)

    Gent, Peter R.; McWilliams, James C.

    1983-07-01

    Scaling analyses are presented which delineate the atmospheric and oceanic regimes of validity for the family of balanced models described in Gent and McWilliams (1983a). The analyses follow and extend the classical work of Charney (1948) and others. The analyses use three non-dimensional parameters which represent the flow scale relative to the Earth's radius, the dominance of turbulent or wave-like processes, and the dominant component of the potential vorticity. For each regime, the models that are accurate both at leading order and through at least one higher order of accuracy in the appropriate small parameter are then identified. In particular, it is found that members of the balanced family are the appropriate models of higher-order accuracy over a broad range of parameter regimes. Examples are also given of particular atmospheric and oceanic phenomena which are in the regimes of validity for the different balanced models.

  14. The international climate regime: towards consolidation collapse

    International Nuclear Information System (INIS)

    This article deals with the different modalities that exist to manage a problem of collective action in the field of climate negotiation. It uses two concepts of the International Political Economy (IPE): the concept of International Regime (IR) and the concept of Hegemony and / or Leadership. The course the international negotiation has taken between 1992 (Rio Convention) and march 2001 (the US rejection of the Kyoto Protocol of 1997) leads us, first, to question the conditions of existence as well as the viability of a non-hegemonic International Regime (Part One). Then, we discuss the perspectives for the 'post - Kyoto' era. After having examined the preferences of the three most active actors in the negotiation (USA, Europe, G77 + China) combined with the leadership capacities they possess, we identify three scenarios for the future: i) anarchy, ii) an international regime under the American hegemony, iii) an international regime under the European leadership (Part Two). (author)

  15. Developmental Regimes in Africa synthesis report

    OpenAIRE

    Booth, D.; Dietz, A.J.; Golooba-Mutebi, F.; Fuady, A.H.; Henley, D; Kelsall, T.; Leliveld, A.H.M.; Donge, van, J.K.

    2015-01-01

    Many African countries have experienced sustained economic growth, but few have achieved the type of structural change, driven by rising productivity, that has transformed mass living standards in parts of Asia. In the Developmental Regimes in Africa Synthesis Report, editor David Booth examines how DRA research has shed new light on how developmental regimes might emerge and be sustained in Africa in the 21st century. He outlines a concept with defining features at three levels: policy conte...

  16. Regime shifts and inflation uncertainty in Peru

    OpenAIRE

    Paúl Castillo; Alberto Humala; Vicente Tuesta

    2012-01-01

    The link between inflation and inflation uncertainty is evaluated using Peruvian data, in a context of changing monetary policies because of regime shifts. A Markov regime-switching heteroskedasticity model that includes unobserved components is used. The model shows how periods of high (low) inflation accompany periods of high (low) short- and long-run uncertainty in inflation. The results of the model also illustrate how, during the recent period of price stability in Peru, both permanent a...

  17. Regime Diagrams for K-Theory Dispersion

    Science.gov (United States)

    Smith, Ronald B.

    2011-06-01

    In atmospheric dispersion, the "non-Gaussian" effects of gravitational settling, the vertical gradient in diffusivity and the surface deposition do not enter uniformly but rather break up parameter space into several discrete regimes. Here, we describe regime diagrams that are constructed for K-theory dispersion of effluent from a surface line source in unsheared inhomogeneous turbulence, using a previously derived Fourier-Hankel method. This K-theory formulation differs from the traditional one by keeping a non-zero diffusivity at the ground. This change allows for turbulent exchange between the canopy and the atmosphere and allows new natural length scales to emerge. The axes on the regime diagrams are non-dimensional distance defined as the ratio of downwind distance to the characteristic length scale for each effect. For each value of the ratio of settling speed to the K gradient, two to four regimes are found. Concentration formulae are given for each regime. The regime diagrams allow real dispersion problems to be categorized and the validity of end-state concentration formulae to be judged.

  18. 尿激酶和肝素钠在断指再植术后的应用%Comparison of curative effect of urokinase and heparin sodium after digital replantation

    Institute of Scientific and Technical Information of China (English)

    吴晓华; 樊涛; 严明忠

    2013-01-01

    Objective To contrast the curative effect of urokinase and heparin sodium after digital replantation.Methods All of 259 patients were randomly divided into urokinase group with 130 cases(217 fingers) and Heparin sodium group with 129 cases (199 fingers).Two groups received the same basic therapy,and respectively injected with urokinase and heparin sodium.The incidence rate of vascular crisis,survival percent of fingers and the recovery of finger function of two group were compared.Results The incidence rate of vascular crisis was 26.7 % and 36.7 % (P < 0.05),the survival percent of fingers was 88.9 % and 81.9 % (P < 0.05),the excellent and good rate of finger function was 69.3 % and 53.8% (P < 0.05),in 2 groups respectively.Conclusion Urokinase has more advantages than heparin sodium in treatment after digital replantation,with reducing the occurrence of vascular crisis,improving the survival rate of replantation of finger and promoting the finger function recovery.%目的 对比分析尿激酶和肝素钠应用在断指再植术后的治疗效果.方法 将259例断指伤患者随机分成尿激酶组130例(217指)和肝素钠组129例(199指),在基础治疗相同的情况下,分别使用尿激酶、肝素钠对再植术后断指进行治疗,比较2组的血管危象发生率、患指成活率、功能恢复等情况.结果 在尿激酶组、肝素钠组中,血管危象发生率分别为26.7%和36.7%(P<0.05),患指成活率分别为88.9%和81.9% (P <0.05),活动功能优良率分别为69.3%和53.8% (P <0.05).结论 在断指再植术后治疗中,尿激酶较肝素钠更能减少血管危象的发生率,提高患指的成活率,促进患指功能恢复.

  19. Laser-Nucleus Interactions: The Quasiadiabatic Regime

    CERN Document Server

    Pálffy, Adriana; Hoefer, Axel; Weidenmüller, Hans A

    2015-01-01

    The interaction between nuclei and a strong zeptosecond laser pulse with coherent MeV photons is investigated theoretically. We provide a first semi-quantitative study of the quasiadiabatic regime where the photon absorption rate is comparable to the nuclear equilibration rate. In that regime, multiple photon absorption leads to the formation of a compound nucleus in the so-far unexplored regime of excitation energies several hundred MeV above the yrast line. The temporal dynamics of the process is investigated by means of a set of master equations that account for dipole absorption, stimulated dipole emission, neutron decay and induced fission in a chain of nuclei. That set is solved numerically by means of state-of-the-art matrix exponential methods also used in nuclear fuel burnup and radioactivity transport calculations. Our quantitative estimates predict the excitation path and range of nuclei reached by neutron decay and provide relevant information for the layout of future experiments.

  20. Fermi's golden rule beyond the Zeno regime

    Science.gov (United States)

    Debierre, Vincent; Goessens, Isabelle; Brainis, Edouard; Durt, Thomas

    2015-08-01

    We reconsider the problem of the spontaneous emission of light by an excited atomic state. We scrutinize the survival probability of this excited state for very short times, in the so-called Zeno regime, for which we show that the dynamics is dictated by a coherent, in-phase, response of the on-shell and off-shell vacuum modes. We also develop a perturbative approach in order to interpolate between different temporal regimes: the Zeno, golden rule (linear), and Wigner-Weisskopf (exponential) regimes. We compare results obtained with the E ̂.x ̂ and A ̂.p ̂ interaction Hamiltonians, using successively the dipole approximation and the exact coupling.

  1. Interpreting MAD within multiple accretion regimes

    CERN Document Server

    Mocz, Philip

    2014-01-01

    General relativistic magnetohydrodynamic (GRMHD) simulations of accreting black holes in the radiatively inefficient regime show that systems with sufficient magnetic poloidal flux become magnetically arrested disc (MAD) systems, with a well-defined relationship between the magnetic flux and the mass accretion rate. Recently, Zamaninasab (2014) report that the jet magnetic flux and accretion disc luminosity are tightly correlated over 7 orders of magnitude for a sample of 76 radio-loud active galaxies, concluding that the data are explained by the MAD mode of accretion. Their analysis assumes radiatively efficient accretion, and their sample consists primarily of radiatively efficient sources, while GRMHD simulations of MAD thus far have been carried out in the radiatively inefficient regime. We propose a model to interpret MAD systems in the context of multiple accretion regimes, and apply it to the sample in Zamaninasab (2014), along with additional radiatively inefficient sources from archival data. We sho...

  2. Regimes of turbulence without an energy cascade

    CERN Document Server

    Barenghi, C F; Baggaley, A W

    2016-01-01

    Experiments and numerical simulations of turbulent $^4$He and $^3$He-B have established that, at hydrodynamic length scales larger than the average distance between quantum vortices, the energy spectrum obeys the same 5/3 Kolmogorov law which is observed in the homogeneous isotropic turbulence of ordinary fluids. The importance of the 5/3 law is that it points to the existence of a Richardson energy cascade from large eddies to small eddies. However, there is also evidence of quantum turbulent regimes without Kolmogorov scaling. This raises the important questions of why, in such regimes, the Kolmogorov spectrum fails to form, what is the physical nature of turbulence without energy cascade, and whether hydrodynamical models can account for the unusual behaviour of turbulent superfluid helium. In this work we describe simple physical mechanisms which prevent the formation of Kolmogorov scaling in the thermal counterflow, and analyze the conditions necessary for emergence of quasiclassical regime in quantum tu...

  3. Early detection of ecosystem regime shifts

    DEFF Research Database (Denmark)

    Lindegren, Martin; Dakos, Vasilis; Groeger, Joachim P.;

    2012-01-01

    Critical transitions between alternative stable states have been shown to occur across an array of complex systems. While our ability to identify abrupt regime shifts in natural ecosystems has improved, detection of potential early-warning signals previous to such shifts is still very limited...... methods may have limited utility in ecosystem-based management as they show no or weak potential for early-warning. We therefore propose a multiple method approach for early detection of ecosystem regime shifts in monitoring data that may be useful in informing timely management actions in the face....... Using real monitoring data of a key ecosystem component, we here apply multiple early-warning indicators in order to assess their ability to forewarn a major ecosystem regime shift in the Central Baltic Sea. We show that some indicators and methods can result in clear early-warning signals, while other...

  4. Determination of the Hall Thruster Operating Regimes

    Energy Technology Data Exchange (ETDEWEB)

    L. Dorf; V. Semenov; Y. Raitses; N.J. Fisch

    2002-04-09

    A quasi one-dimensional (1-D) steady-state model of the Hall thruster is presented. For the same discharge voltage two operating regimes are possible -- with and without the anode sheath. For given mass flow rate, magnetic field profile and discharge voltage a unique solution can be constructed, assuming that the thruster operates in one of the regimes. However, we show that for a given temperature profile the applied discharge voltage uniquely determines the operating regime: for discharge voltages greater than a certain value, the sheath disappears. That result is obtained over a wide range of incoming neutral velocities, channel lengths and widths, and cathode plane locations. It is also shown that a good correlation between the quasi 1-D model and experimental results can be achieved by selecting an appropriate electron mobility and temperature profile.

  5. Soluble Urokinase Receptor Levels Are Correlated with Focal Segmental Glomerulosclerosis Lesions in IgA Nephropathy: A Cohort Study from China.

    Directory of Open Access Journals (Sweden)

    Shui-Ming Guo

    Full Text Available Soluble urokinase receptor (suPAR may be involved in the pathological mechanisms of focal segmental glomerulosclerosis (FSGS changes. However, it remains unclear whether suPAR is correlated with the FSGS-like lesions in IgA nephropathy (IgAN.We measured the plasma suPAR levels in 138 patients with IgAN, and then their clinical and pathological relationships were analyzed.We found that the plasma suPAR levels were significantly correlated with age and renal function by both univariate and multivariate analysis in our IgAN patient cohort. Female had higher plasma suPAR levels and no significant correlation was observed between plasma suPAR levels and 24-h urine protein and highly sensitive C-reaction protein with multivariate analysis. In our cohort, sixty of these IgAN patients could be diagnosed with a type of FSGS lesions. The plasma suPAR levels were higher in the IgAN patients with FSGS lesions than in the IgAN patients without FSGS lesions by univariate (P < 0.0001 and multivariate (P < 0.001 analysis adjusting for other predictor variables, which might be helpful to differentiate the pathological changes with and without FSGS lesions. And the optimal cutoff value was 1806 pg/ml in this study. The plasma suPAR concentrations were also associated with the degree of tubular atrophy/interstitial fibrosis in both univariate and multivariate analysis. In multivariate analysis, the plasma suPAR levels were correlated with the percentage of crescents, not global sclerosis and arterial lesions.Our study suggested that the plasma suPAR levels were associated with age, gender, renal function, the degree of tubular atrophy/interstitial fibrosis and the percentage of crescent formation. The plasma suPAR might be a potential predictor for the presence of FSGS pathological lesions in Chinese patients with IgAN.

  6. Plasma levels of soluble urokinase-type plasminogen activator receptor (suPAR and early mortality risk among patients enrolling for antiretroviral treatment in South Africa

    Directory of Open Access Journals (Sweden)

    Bangani Nonzwakazi

    2007-05-01

    Full Text Available Abstract Background Serum concentrations of soluble urokinase-type plasminogen activator receptor (suPAR have a strong independent association with HIV-1-related mortality. The practical utility of plasma suPAR in assessing short-term all-cause mortality risk was evaluated in patients with advanced immunodeficiency enrolling in an antiretroviral treatment (ART programme in South Africa. Methods An enzyme-linked immunosorbent assay (ELISA was used to measure plasma concentrations of suPAR in patients at the time of enrolment to the ART programme. The association between plasma suPAR concentrations, baseline patient characteristics and cohort outcomes after 4 months of ART were determined. Results Patients (n = 293, 70% female had a median age of 33 years and were followed up for a median of 5 months from enrolment. The median CD4 cell count was 47 cells/μl (IQR = 22–72 and 38% of patients had WHO stage 4 disease. 218 (74% patients remained alive after 4 months of ART; 39 (13% died and 36 (12% were lost to the programme for other reasons. Patients who died had significantly higher plasma suPAR concentrations compared to those who either survived (P 10 suPAR concentrations were significantly associated with lower CD4 cell counts, WHO clinical stage 4 disease and male sex. In multivariate analysis to identify factors associated with death, log10 suPAR concentration was the most strongly associated variable (P Conclusion Plasma suPAR concentration was the strongest independent predictor of short-term mortality risk among patients with advanced immunodeficiency enrolling in this ART programme. However, lack of a discriminatory threshold did not permit this marker to be used to triage patients according to short-term mortality risk.

  7. Concentrations of plasminogen activator inhibitor-1 (PAI-1 and urokinase plasminogen activator (uPA in induced sputum of asthma patients after allergen challenge

    Directory of Open Access Journals (Sweden)

    Krzysztof Kowal,

    2010-04-01

    Full Text Available Urokinase plasminogen activator (uPA and its inhibitor (PAI-1 are involved in tiisue remodeling and repairprocesses associated with acute and chronic inflammation. The aim of the study was to evaluate the effect of allergen challengeon concentration of uPA and PAI-1 in induced sputum of house dust mite allergic asthmatics (HDM-AAs. ThirtyHDM-AAs and ten healthy persons (HCswere recruited for the study. In 24 HDM-AAs bronchial challenge with Dermatophagoidespteronyssinus (Dp and in 6 HDM-AAs sham challenege with saline were performed. In HDM-AAs sputumwas induced 24 hours before (T0 and 24 hours (T24 after the challenge. Concentration of uPA and PAI-1 in induced sputumwere determined using immunoenzymatic assays. At T0 in HDM-AAs mean sputum uPA (151±96 pg/ml and PAI-1(4341±1262 pg/ml concentrations were higher than in HC (18.8±6.7 pg/ml; p=0.0002 and 596±180 pg/ml; p<0.0001; foruPA and PAI-1 respectively. After allergen challenge further increase in sputum uPA (187±144 pg/ml; p=0.03 and PAI-1(6252±2323 pg/ml; p<0.0001 concentrations were observed. Moreover, in Dp challenged, but not in saline challengedHDM-AAs the mean uPA/PAI-1 ratio decreased significantly at T24. No significant increase in the studied parameters werefound in sham challenged patients. In HDM-AAs allergen exposure leads to activation of the plasmin system in the airways.Greater increase of the PAI-1 concentration than uPA concentration after allergen challenge may promote airway remodelingand play an important role in the development of bronchial hyperreactivity.

  8. Concentrations of plasminogen activator inhibitor-1 (PAI-1 and urokinase plasminogen activator (uPA in induced sputum of asthma patients after allergen challenge.

    Directory of Open Access Journals (Sweden)

    Marcin Moniuszko

    2011-04-01

    Full Text Available Urokinase plasminogen activator (uPA and its inhibitor (PAI-1 are involved in tiisue remodeling and repair processes associated with acute and chronic inflammation. The aim of the study was to evaluate the effect of allergen challenge on concentration of uPA and PAI-1 in induced sputum of house dust mite allergic asthmatics (HDM-AAs. Thirty HDM-AAs and ten healthy persons (HCswere recruited for the study. In 24 HDM-AAs bronchial challenge with Dermatophagoides pteronyssinus (Dp and in 6 HDM-AAs sham challenege with saline were performed. In HDM-AAs sputum was induced 24 hours before (T0 and 24 hours (T24 after the challenge. Concentration of uPA and PAI-1 in induced sputum were determined using immunoenzymatic assays. At T0 in HDM-AAs mean sputum uPA (151 Âą 96 pg/ml and PAI-1 (4341 Âą 1262 pg/ml concentrations were higher than in HC (18.8 Âą 6.7 pg/ml; p=0.0002 and 596 Âą 180 pg/ml; p<0.0001; for uPA and PAI-1 respectively. After allergen challenge further increase in sputum uPA (187 Âą 144 pg/ml; p=0.03 and PAI-1 (6252 Âą 2323 pg/ml; p<0.0001 concentrations were observed. Moreover, in Dp challenged, but not in saline challenged HDM-AAs the mean uPA/PAI-1 ratio decreased significantly at T24. No significant increase in the studied parameters were found in sham challenged patients. In HDM-AAs allergen exposure leads to activation of the plasmin system in the airways. Greater increase of the PAI-1 concentration than uPA concentration after allergen challenge may promote airway remodeling and play an important role in the development of bronchial hyperreactivity.

  9. The receptor for urokinase-plasminogen activator (uPAR) controls plasticity of cancer cell movement in mesenchymal and amoeboid migration style.

    Science.gov (United States)

    Margheri, Francesca; Luciani, Cristina; Taddei, Maria Letizia; Giannoni, Elisa; Laurenzana, Anna; Biagioni, Alessio; Chillà, Anastasia; Chiarugi, Paola; Fibbi, Gabriella; Del Rosso, Mario

    2014-03-30

    The receptor for the urokinase plasminogen activator (uPAR) is up-regulated in malignant tumors. Historically the function of uPAR in cancer cell invasion is strictly related to its property to promote uPA-dependent proteolysis of extracellular matrix and to open a path to malignant cells. These features are typical of mesenchymal motility. Here we show that the full-length form of uPAR is required when prostate and melanoma cancer cells convert their migration style from the "path generating" mesenchymal to the "path finding" amoeboid one, thus conferring a plasticity to tumor cell invasiveness across three-dimensional matrices. Indeed, in response to a protease inhibitors-rich milieu, prostate and melanoma cells activated an amoeboid invasion program connoted by retraction of cell protrusions, RhoA-mediated rounding of the cell body, formation of a cortical ring of actin and a reduction of Rac-1 activation. While the mesenchymal movement was reduced upon silencing of uPAR expression, the amoeboid one was almost completely abolished, in parallel with a deregulation of small Rho-GTPases activity. In melanoma and prostate cancer cells we have shown uPAR colocalization with β1/β3 integrins and actin cytoskeleton, as well integrins-actin co-localization under both mesenchymal and amoeboid conditions. Such co-localizations were lost upon treatment of cells with a peptide that inhibits uPAR-integrin interactions. Similarly to uPAR silencing, the peptide reduced mesenchymal invasion and almost abolished the amoeboid one. These results indicate that full-length uPAR bridges the mesenchymal and amoeboid style of movement by an inward-oriented activity based on its property to promote integrin-actin interactions and the following cytoskeleton assembly. PMID:24681666

  10. Procedures for the quantitative protein determination of urokinase and its inhibitor, PAI-1, in human breast cancer tissue extracts by ELISA.

    Science.gov (United States)

    Schmitt, Manfred; Sturmheit, Alexandra S; Welk, Anita; Schnelldorfer, Christel; Harbeck, Nadia

    2006-01-01

    The determination of the protein content of urokinase-type plasminogen activator (uPA) and its inhibitor, PAI-1, in breast cancer tissue extracts is used clinically to identify patients at risk to experience disease recurrence (metastasis) or early death. The serine protease uPA, in concert with its inhibitor PAI-1, promotes tumor cell adhesion, migration, and proliferation, as well as extracellular matrix degradation and, thus, facilitates tumor cell invasion and metastasis. The various technical steps to recover uPA and PAI-1 protein from archived breast cancer tissues and to quantitatively determine uPA and PAI-1 protein content in tumor tissue extracts by enzyme-linked immunosorbent assay (ELISA) are described in detail. The technical steps involved require fresh-frozen breast cancer tissue, a dismembrator machine (ball mill) to pulverize the tissue in the frozen state, detergent (Triton X-100) containing Tris-buffered saline to extract uPA and PAI-1 from the pulverized breast cancer tissue, an ultracentrifuge to separate the detergent fraction from cellular debris, uPA and PAI-1 ELISA kits, protein determination reagents, and a 96-well spectrophotometer (ELISA reader) to assess uPA, PAI-1, and total protein in the detergent extract. The uPA/PAI-1 ELISAs and the protein determination format described are robust and highly sensitive. In addition to the macromethod of tissue disintegration, we present a simple but sensitive micro-extraction procedure using cryostat sections or core biopsies as the source of breast cancer tissue. Such a technique allows rapid and quantitative determination of uPA and PAI-1, even in small breast cancer specimens.

  11. Regimes, Non-State Actors and the State System: A 'Structurational' Regime Model

    NARCIS (Netherlands)

    Arts, B.J.M.

    2000-01-01

    Regime analysis has become a popular approach in International Relations theory and in international policy studies. However, current regime models exhibit some shortcomings with regard to (1) addressing non-state actors, and in particular nongovernmental organizations (NGOs), (2) the balancing of a

  12. Causas estruturais e consequências dos regimes internacionais: regimes como variáveis intervenientes

    Directory of Open Access Journals (Sweden)

    Stephen D. Krasner

    2012-06-01

    Full Text Available Os regimes internacionais são definidos como princípios, normas, regras e procedimentos de tomada de decisões ao redor dos quais as expectativas dos atores convergem em uma dada área-tema. Como ponto de partida, os regimes são conceituados como variáveis intervenientes, estando entre fatores causais básicos e os resultados e comportamentos relacionados. Há três visões a respeito da importância dos regimes: as orientações estruturais convencionais desvalorizam os regimes como sendo, na melhor das hipóteses, ineficazes; as orientações grocianas vêem os regimes como componentes íntimos do sistema internacional; as perspectivas estruturalistas modificadas vêem os regimes como significativos somente em certas condições restritas. Para os argumentos grociano e estruturalista modificado - que concordam com a visão de que os regimes podem influenciar resultados e comportamentos - , o desenvolvimento de regimes é visto como uma função de cinco variáveis causais básicas: auto-interesse egoísta; poder político; normas e princípios difusos; usos e costumes; conhecimento.

  13. Supercurrent in the quantum Hall regime.

    Science.gov (United States)

    Amet, F; Ke, C T; Borzenets, I V; Wang, J; Watanabe, K; Taniguchi, T; Deacon, R S; Yamamoto, M; Bomze, Y; Tarucha, S; Finkelstein, G

    2016-05-20

    A promising route for creating topological states and excitations is to combine superconductivity and the quantum Hall (QH) effect. Despite this potential, signatures of superconductivity in the QH regime remain scarce, and a superconducting current through a QH weak link has been challenging to observe. We demonstrate the existence of a distinct supercurrent mechanism in encapsulated graphene samples contacted by superconducting electrodes, in magnetic fields as high as 2 tesla. The observation of a supercurrent in the QH regime marks an important step in the quest for exotic topological excitations, such as Majorana fermions and parafermions, which may find applications in fault-tolerant quantum computing. PMID:27199424

  14. Economic performance and North Korean regime legitimacy

    OpenAIRE

    Moore, Patrick J.

    2014-01-01

    This thesis examines the sources of legitimacy for the North Korean regime in an effort to explain what role, if any, economic performance has played in keeping the Kim family in power. This thesis provides a historical look at the development of the North Korean regime from the beginning under Kim Il-sung to the current generation of rule under Kim Jong-un. The core argument of the thesis is broken into two major time periods, with the economic downturn of the early 1990s serving as the divi...

  15. WELFARE REGIMES IN LATIN AMERICA AND CARIBBEAN

    Directory of Open Access Journals (Sweden)

    Melisa Campana-Alabarce

    2015-06-01

    Full Text Available This article provides a characterization of Latin American and Caribbean Welfare regimes in historiographical perspective. Firstly, it makes a review of the emergence conditions of Welfare States in Western Europe and its core features, with particular emphasis on its role as a method to regulate inequalities in industrial capitalism. Dialoguing with it, then stops in the specific configurations that welfare regimes have taken in Latin America during the course of the twentieth century. Finally, it provides a map of its contemporary features and the major challenges that the States of the region face in his capacity as right guarantors for the future.

  16. Light focusing in the Anderson Regime

    CERN Document Server

    Leonetti, Marco; Mafi, Arash; Conti, Claudio

    2014-01-01

    Anderson localization is a regime in which diffusion is inhibited and waves (also electromagnetic waves) get localized. Here we exploit adaptive optics to achieve focusing in disordered optical fibers in the Anderson regime. By wavefront shaping and optimization, we observe the generation of a propagation invariant beam, where light is trapped transversally by disorder, and show that Anderson localizations can be also excited by extended speckled beams. We demonstrate that disordered fibers allow a more efficient focusing action with respect to standard fibers in a way independent of their length, because of the propagation invariant features and cooperative action of transverse localizations.

  17. Synchronization regimes in conjugate coupled chaotic oscillators.

    Science.gov (United States)

    Karnatak, Rajat; Ramaswamy, Ram; Prasad, Awadhesh

    2009-09-01

    Nonlinear oscillators that are mutually coupled via dissimilar (or conjugate) variables display distinct regimes of synchronous behavior. In identical chaotic oscillators diffusively coupled in this manner, complete synchronization occurs only by chaos suppression when the coupled subsystems drive each other into a regime of periodic dynamics. Furthermore, the coupling does not vanish but acts as an "internal" drive. When the oscillators are mismatched, phase synchronization occurs, while in a master slave configuration, generalized synchrony results. These effects are demonstrated in a system of coupled chaotic Rossler oscillators.

  18. Supercurrent in the quantum Hall regime

    Science.gov (United States)

    Amet, F.; Ke, C. T.; Borzenets, I. V.; Wang, J.; Watanabe, K.; Taniguchi, T.; Deacon, R. S.; Yamamoto, M.; Bomze, Y.; Tarucha, S.; Finkelstein, G.

    2016-05-01

    A promising route for creating topological states and excitations is to combine superconductivity and the quantum Hall (QH) effect. Despite this potential, signatures of superconductivity in the QH regime remain scarce, and a superconducting current through a QH weak link has been challenging to observe. We demonstrate the existence of a distinct supercurrent mechanism in encapsulated graphene samples contacted by superconducting electrodes, in magnetic fields as high as 2 tesla. The observation of a supercurrent in the QH regime marks an important step in the quest for exotic topological excitations, such as Majorana fermions and parafermions, which may find applications in fault-tolerant quantum computing.

  19. Anomalous Hall effect in localization regime

    Science.gov (United States)

    Wu, Lin; Zhu, Kai; Yue, Di; Tian, Yuan; Jin, Xiaofeng

    2016-06-01

    The anomalous Hall effect in the ultrathin film regime is investigated in Fe(001)(1-3 nm) films epitaxial on MgO(001). The logarithmic localization correction to longitudinal resistivity and anomalous Hall resistivity are observed at low temperature. We identify that the coefficient of skew scattering has a reduction from metallic to localized regime, while the contribution of side jump has inconspicuous change except for a small drop below 10 K. Furthermore, we discover that the intrinsic anomalous Hall conductivity decreases with the reduction of thickness below 2 nm. Our results provide unambiguous experimental evidence to clarify the problem of localization correction to the anomalous Hall effect.

  20. The oil tax regime of Azerbaijan

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, Gerard

    1998-07-01

    Azerbaijan has a long history in the oil business and a chance of a spectacular future. To understand why the oil tax regime evolved into its present form and how it is likely to develop, it is necessary to know something of the country's history and the commercial environment. Consequently the presentation begins by discussing these items. It then outlines the Production Sharing Agreement regime in Azerbaijan and then deals with the Kazakh and Georgian Tax Codes, as these are likely to be the basis of a new general tax law in Azerbaijan from 1999. The presentation includes comments on the New Draft Tax Code of 1998.

  1. Effect of vacuum-assisted closure on the expression of urokinase-type plaminogen activator and urokinase-type plasminogen activator receptor in acute and chronic wounds healing%封闭负压引流技术对创面愈合过程纤溶酶原激活剂级联表达的影响

    Institute of Scientific and Technical Information of China (English)

    李跃军; 曹大勇; 陈绍宗

    2006-01-01

    目的研究封闭负压引流技术对急慢性创面纤溶酶原激活剂(plasminogen activator,PA)级联中尿激酶型纤溶酶激活剂(urokinase-type plaminogen activator,uPA)和尿激酶型纤溶酶激活剂受体(urokinase-type plasminogen activator receptor,uPA)表达的影响.方法雄性小家猪5头,背部两侧形成急性创面,分为实验组和对照组,仅实验组动物接受封闭负压引流治疗.分别在治疗前和治疗后第1、3、6、9、12、18、25天于创缘切取标本,用兔抗人uPA和uPAR单克隆抗体按ABC程序进行免疫组织化学染色,并计算uPAA和uPAR的标记指数.人慢性创面6例,清创后进行持续封闭负压引流治疗,在治疗后第1、3、5、7天采集创面渗出液,并用ELISA法检测渗出液中uPA和uPAR的含量.结果猪急性创面在封闭负压引流治疗后uPA和uPAR均增加,在第3天达到高峰,然后急速下降,但实验组的基因表达量和染色强度均显著高于对照组.6例人慢性创面进行治疗前uPA和uPAR表达较多,随着治疗时间的延长而逐渐减少.结论封闭负压引流治疗既能上调急性创面伤口周围的表皮角质形成细胞的uPA和uPAR表达,使之迅速增殖迁移;又能通过抑制慢性创面uPA和uPAR表达,从而减少细胞外基质的降解.

  2. Regimes of flow past a vortex generator

    DEFF Research Database (Denmark)

    Velte, Clara Marika; Okulov, V.L.; Naumov, I.V.

    2012-01-01

    particle image velocimetry (SPIV). Based on the obtained SPIV data, a map of the regimes of flow past the vortex generator has been constructed. One region with a developed stable multivortex system on this map reaches the vicinity of the optimum angle of attack of the vortex generator....

  3. In Search of the Chiral Regime

    CERN Document Server

    Beane, S R

    2004-01-01

    A critical appraisal is given of a recent analysis of the quark-mass and finite-size dependence of unquenched lattice QCD data for the nucleon mass. We use this forum to estimate the boundary of the chiral regime for nucleon properties.

  4. Optimal dividend distribution under Markov regime switching

    NARCIS (Netherlands)

    Z. Jiang; M. Pistorius

    2012-01-01

    We investigate the problem of optimal dividend distribution for a company in the presence of regime shifts. We consider a company whose cumulative net revenues evolve as a Brownian motion with positive drift that is modulated by a finite state Markov chain, and model the discount rate as a determini

  5. European welfare regimes: Political orientations versus poverty

    Directory of Open Access Journals (Sweden)

    Josifidis Kosta

    2011-01-01

    Full Text Available This inquiry analyzes how political orientations shape welfare states and labour market institutions when seeking to reduce poverty. In order to identify effects of these two key variables, we conduct a panel regression analysis that includes two poverty measures: poverty rates before and after social spending. This inquiry considers 14 EU countries, and in the period from 1995 to 2008, which are grouped according to welfare state regimes. We consider Social Democratic, Corporatist, Mediterranean and Liberal welfare state regimes. Panel regression results indicate that political orientation engenders no significant statistically measurable effects on poverty rates before social spending. Effects register, however, as significant when considering poverty rates after social spending. With respect to the first set of results, we advance two key explanations. First, we note a longer period of time is necessary in order to observe actual effects of political orientation on market generated poverty. Second, political parties with their respective programs do not register as influential enough to solve social problems related to income distribution when taken alone. Influences register as indirect and are expressed through changes in employment rates and social spending. The second set of results support the hypothesis that a selected political regime does indeed contribute to poverty reduction. In sum, political orientation and political regime does indeed affect poverty through welfare state institutions, as well as through labour market institutions.

  6. Estimation in autoregressive models with Markov regime

    OpenAIRE

    Ríos, Ricardo; Rodríguez, Luis

    2005-01-01

    In this paper we derive the consistency of the penalized likelihood method for the number state of the hidden Markov chain in autoregressive models with Markov regimen. Using a SAEM type algorithm to estimate the models parameters. We test the null hypothesis of hidden Markov Model against an autoregressive process with Markov regime.

  7. A Comparative Typology of Pension Regimes

    NARCIS (Netherlands)

    Arjan Soede; Cok Vrooman

    2008-01-01

    This report presents an empirical typology of pension regimes in the European Union, the US, Canada, Australia and Norway. The categorisation is based on 34 quantitative and qualitative characteristics of the mandatory parts of the pension systems in these countries. The empirical analysis shows tha

  8. Developmental Regimes in Africa synthesis report

    NARCIS (Netherlands)

    Booth, D.; Dietz, A.J.; Golooba-Mutebi, F.; Fuady, A.H.; Henley, D.; Kelsall, T.; Leliveld, A.H.M.; Donge, van J.K.

    2015-01-01

    Many African countries have experienced sustained economic growth, but few have achieved the type of structural change, driven by rising productivity, that has transformed mass living standards in parts of Asia. In the Developmental Regimes in Africa Synthesis Report, editor David Booth examines how

  9. Radiative effects of global MODIS cloud regimes

    Science.gov (United States)

    Oreopoulos, Lazaros; Cho, Nayeong; Lee, Dongmin; Kato, Seiji

    2016-03-01

    We update previously published Moderate Resolution Imaging Spectroradiometer (MODIS) global cloud regimes (CRs) using the latest MODIS cloud retrievals in the Collection 6 data set. We implement a slightly different derivation method, investigate the composition of the regimes, and then proceed to examine several aspects of CR radiative appearance with the aid of various radiative flux data sets. Our results clearly show that the CRs are radiatively distinct in terms of shortwave, longwave, and their combined (total) cloud radiative effect. We show that we can clearly distinguish regimes based on whether they radiatively cool or warm the atmosphere, and thanks to radiative heating profiles, to discern the vertical distribution of cooling and warming. Terra and Aqua comparisons provide information about the degree to which morning and afternoon occurrences of regimes affect the symmetry of CR radiative contribution. We examine how the radiative discrepancies among multiple irradiance data sets suffering from imperfect spatiotemporal matching depend on CR and whether they are therefore related to the complexity of cloud structure, its interpretation by different observational systems, and its subsequent representation in radiative transfer calculations.

  10. De facto and official exchange rate regimes in transition economies

    OpenAIRE

    von Hagen, Jürgen; Zhou, Jizhong

    2002-01-01

    This paper provides an empirical investigation on the discrepancies between official exchange rate regimes and de facto exchange rate policies in transition economies. Since official and de facto regime choices are not independent of each other, we adopt a bivariate probit model to describe the joint determination of the two regime choices. After finding the important determinants of both regime choices, we use a univariate probit model to describe the determination of regime discrepancies. W...

  11. The Effect of Market Regimes on Style Allocation

    OpenAIRE

    Ammann, Manuel; Verhofen, Michael

    2006-01-01

    We analyse time-varying risk premia and the implications for portfolio choice. Using Markov Chain Monte Carlo (MCMC) methods, we estimate a multivariate regime-switching model for the Carhart (1997) four factor model. We find two clearly separable Regimes with different mean returns, volatilities and correlations. In the High-Variance Regime, only value stocks deliver a good performance, whereas in the Low-Variance Regime, the market portfolio and momentum stocks promise high returns. Regime-...

  12. Effect Observation of Small Dose Urokinase in Treatment of Thrombolytic Therapy of Acute Myocardial Infarction%小剂量尿激酶在急性心肌梗塞溶栓治疗中的疗效分析

    Institute of Scientific and Technical Information of China (English)

    刘兴君

    2015-01-01

    目的:小剂量尿激酶在心肌梗死溶栓治疗中的疗效分析。方法选取2012年7月—2014年4月该院收治的急性心肌梗塞患者75例,并分为常规组(n=38)和小剂量组(n=37)。常规组给予150U尿激酶治疗,小剂量组给予50U尿激酶治疗,比较两组的溶栓再通率、急性期死亡率和溶栓不良反应。结果常规组溶栓总再通率为84.21%,显著高于小剂量组45.94%(c2=12.119,P=0.000);常规治疗组的急性期死亡率为2.63%,显著低于小剂量组33.33%(c2=4.730,P=0.030)。常规剂量组,溶栓治疗后不良发生率为18.42%,小剂量组则为18.91%,两组比较,差异无统计学意义(c2=0.003,P=0.956)。结论在急性心肌梗塞溶栓治疗中,需给予足量的尿激酶,小剂量的治疗效果不甚理想。%Objective To investigate effect of small dose urokinase in treatment of thrombolytic therapy of acute myocardial infarction.Methods 75 patients from 2012 July to 2014 April in our hospital were selected,and were divided into two groups.The routine group was given 150U urokinase,while small dose group was given 50U urokinase.Thrombolytic recanalization rate, acute mortality and adverse reaction rate of two groups were compared.Results Thrombolysis total recanalization rate of routine group was 84.21%,which was significantly higher than the small dose group(45.94%)(c2=12.119,P=0.000). Thrombolysis total recanalization rate of routine group was 2.63%,which was significantly lower than that of the small dose group(18.91%) (c2=4.730,P=0.030). The adverse reaction rate of routine group was 18.42%,while routine group was 18.91%,and there was no significant difference between two groups(c2=0.003,P=0.956).Conclusion In the thrombolytic therapy of acute myocardial infarction treatment, enough small dose urokinase should be given, and treatment effect of small dose of urokinase is not very ideal.

  13. Regime shifts in the anthropocene: drivers, risks, and resilience.

    Science.gov (United States)

    Rocha, Juan Carlos; Peterson, Garry D; Biggs, Reinette

    2015-01-01

    Many ecosystems can experience regime shifts: surprising, large and persistent changes in the function and structure of ecosystems. Assessing whether continued global change will lead to further regime shifts, or has the potential to trigger cascading regime shifts has been a central question in global change policy. Addressing this issue has, however, been hampered by the focus of regime shift research on specific cases and types of regime shifts. To systematically assess the global risk of regime shifts we conducted a comparative analysis of 25 generic types of regime shifts across marine, terrestrial and polar systems; identifying their drivers, and impacts on ecosystem services. Our results show that the drivers of regime shifts are diverse and co-occur strongly, which suggests that continued global change can be expected to synchronously increase the risk of multiple regime shifts. Furthermore, many regime shift drivers are related to climate change and food production, whose links to the continued expansion of human activities makes them difficult to limit. Because many regime shifts can amplify the drivers of other regime shifts, continued global change can also be expected to increase the risk of cascading regime shifts. Nevertheless, the variety of scales at which regime shift drivers operate provides opportunities for reducing the risk of many types of regime shifts by addressing local or regional drivers, even in the absence of rapid reduction of global drivers. PMID:26267896

  14. A combination of desmopressin and docetaxel inhibit cell proliferation and invasion mediated by urokinase-type plasminogen activator (uPA) in human prostate cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Sasaki, Hiroshi; Klotz, Laurence H. [Division of Urology, Sunnybrook Health Sciences Center, Toronto, ON (Canada); Sugar, Linda M. [Department of Pathology, Sunnybrook Health Sciences Center, Toronto, ON (Canada); Kiss, Alexander [Department of Research Design and Biostatistics, Institute for Clinical Evaluative Sciences, Sunnybrook Health Sciences Center, Toronto, ON (Canada); Venkateswaran, Vasundara, E-mail: vasundara.venkateswaran@sunnybrook.ca [Division of Urology, Sunnybrook Health Sciences Center, Toronto, ON (Canada)

    2015-08-28

    Background: This study was designed to assess the effectiveness of a combination treatment using both desmopressin and docetaxel in prostate cancer treatment. Desmopressin is a well-known synthetic analogue of the antidiuretic hormone vasopressin. It has recently been demonstrated to inhibit tumor progression and metastasis in in vivo models. Docetaxel is widely used for the treatment of castration resistant prostate cancer (CRPC) patients. However, durable responses have been uncommon to date. In this study, we investigated the anti-tumor effect of desmopressin in combination with docetaxel in vitro and in vivo. Methods: Two prostate cancer cells (PC3, LNCaP) were treated with different concentrations of desmopressin alone, docetaxel alone, and a combination of desmopressin and docetaxel. Cell proliferation was determined by MTS assay. The anti-invasive and anti-migration potential of desmopressin and in combination with docetaxel were examined by wound healing assay, migration chamber assay, and matrigel invasion assay. Results: The combination of desmopressin and docetaxel resulted in a significant inhibition of PC3 and LNCaP cell proliferation (p < 0.01). Additionally, cell migration and invasion were also inhibited by the combination when compared to that of either treatment alone in PC3 cells (p < 0.01). The anti-tumor effect of this combination treatment was associated with down-regulation of both urokinase-type plasminogen activator (uPA) and matrix metalloproteinase (MMP-2 and MMP-9) in PC3 cells. Conclusions: We are the first to elucidate the anti-tumor and anti-metastatic potential of desmopressin in combination with docetaxel in a prostate cancer model via the uPA-MMP pathway. Our finding could potentially contribute to the therapeutic profile of desmopressin and enhance the efficacy of docetaxel based treatment for CRPC. - Highlights: • Desmopressin inhibits cell proliferation in prostate cancer cells. • The expression of cyclin A and CDK2

  15. Urokinase plasminogen activator inhibits HIV virion release from macrophage-differentiated chronically infected cells via activation of RhoA and PKCε.

    Directory of Open Access Journals (Sweden)

    Francesca Graziano

    Full Text Available BACKGROUND: HIV replication in mononuclear phagocytes is a multi-step process regulated by viral and cellular proteins with the peculiar feature of virion budding and accumulation in intra-cytoplasmic vesicles. Interaction of urokinase-type plasminogen activator (uPA with its cell surface receptor (uPAR has been shown to favor virion accumulation in such sub-cellular compartment in primary monocyte-derived macrophages and chronically infected promonocytic U1 cells differentiated into macrophage-like cells by stimulation with phorbol myristate acetate (PMA. By adopting this latter model system, we have here investigated which intracellular signaling pathways were triggered by uPA/uPAR interaction leading the redirection of virion accumulation in intra-cytoplasmic vesicles. RESULTS: uPA induced activation of RhoA, PKCδ and PKCε in PMA-differentiated U1 cells. In the same conditions, RhoA, PKCδ and PKCε modulated uPA-induced cell adhesion and polarization, whereas only RhoA and PKCε were also responsible for the redirection of virions in intracellular vesicles. Distribution of G and F actin revealed that uPA reorganized the cytoskeleton in both adherent and polarized cells. The role of G and F actin isoforms was unveiled by the use of cytochalasin D, a cell-permeable fungal toxin that prevents F actin polymerization. Receptor-independent cytoskeleton remodeling by Cytochalasin D resulted in cell adhesion, polarization and intracellular accumulation of HIV virions similar to the effects gained with uPA. CONCLUSIONS: These findings illustrate the potential contribution of the uPA/uPAR system in the generation and/or maintenance of intra-cytoplasmic vesicles that actively accumulate virions, thus sustaining the presence of HIV reservoirs of macrophage origin. In addition, our observations also provide evidences that pathways controlling cytoskeleton remodeling and activation of PKCε bear relevance for the design of new antiviral strategies aimed

  16. Direct interaction of the kringle domain of urokinase-type plasminogen activator (uPA) and integrin alpha v beta 3 induces signal transduction and enhances plasminogen activation.

    Science.gov (United States)

    Tarui, Takehiko; Akakura, Nobuaki; Majumdar, Mousumi; Andronicos, Nicholas; Takagi, Junichi; Mazar, Andrew P; Bdeir, Khalil; Kuo, Alice; Yarovoi, Serge V; Cines, Douglas B; Takada, Yoshikazu

    2006-03-01

    It has been questioned whether there are receptors for urokinase-type plasminogen activator (uPA) that facilitate plasminogen activation other than the high affinity uPA receptor (uPAR/CD87) since studies of uPAR knockout mice did not support a major role of uPAR in plasminogen activation. uPA also promotes cell adhesion, chemotaxis, and proliferation besides plasminogen activation. These uPA-induced signaling events are not mediated by uPAR, but mediated by unidentified, lower-affinity receptors for the uPA kringle. We found that uPA binds specifically to integrin alpha v beta 3 on CHO cells depleted of uPAR. The binding of uPA to alpha v beta 3 required the uPA kringle domain. The isolated uPA kringle domain binds specifically to purified, recombinant soluble, and cell surface alpha v beta 3, and other integrins (alpha 4 beta 1 and alpha 9 beta 1), and induced migration of CHO cells in an alpha v beta 3-dependent manner. The binding of the uPA kringle to alpha v beta 3 and uPA kringle-induced alpha v beta 3-dependent cell migration were blocked by homologous plasminogen kringles 1-3 or 1-4 (angiostatin), a known integrin antagonist. We studied whether the binding of uPA to integrin alpha v beta 3 through the kringle domain plays a role in plasminogen activation. On CHO cell depleted of uPAR, uPA enhanced plasminogen activation in a kringle and alpha v beta 3-dependent manner. Endothelial cells bound to and migrated on uPA and uPA kringle in an alpha v beta 3-dependent manner. These results suggest that uPA binding to integrins through the kringle domain plays an important role in both plasminogen activation and uPA-induced intracellular signaling. The uPA kringle-integrin interaction may represent a novel therapeutic target for cancer, inflammation, and vascular remodeling. PMID:16525582

  17. Recent Activities on Global Nuclear Safety Regime

    International Nuclear Information System (INIS)

    Recently, rapid progress on the globalization of the nuclear safety issues is being made in IAEA (International Atomic Energy Agency) and its member states. With the globalization, the need for international cooperation among international bodies and member states continues to grow for resolving these universal nuclear safety issues. Furthermore, the importance of strengthening the global nuclear safety regime is emphasized through various means, such as efforts in application of IAEA safety standards to all nuclear installations in the world and in strengthening the code of conduct and the convention on nuclear safety. In this regards, it is important for us to keep up with the activities related with the global nuclear safety regime as an IAEA member state and a leading country in nuclear safety regulation

  18. Online recognition of the multiphase flow regime

    Institute of Scientific and Technical Information of China (English)

    BAI BoFeng; ZHANG ShaoJun; ZHAO Liang; ZHANG XiMin; GUO LieJin

    2008-01-01

    The key reasons that the present method cannot be used to solve the industrial multi-phase flow pattern recognition are clarified firstly. The prerequisite to realize the online recognition is proposed and recognition rules for partial flow pattern are obtained based on the massive experimental data. The standard templates for every flow regime feature are calculated with self-organization cluster algorithm. The multi-sensor data fusion method is proposed to realize the online recognition of multiphase flow regime with the pressure and differential pressure signals, which overcomes the severe influence of fluid flow velocity and the oil fraction on the recognition. The online recognition method is tested in the practice, which has less than 10 percent measurement error. The method takes advantages of high confidence, good fault tolerance and less requirement of single sensor performance.

  19. Online recognition of the multiphase flow regime

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The key reasons that the present method cannot be used to solve the industrial multi- phase flow pattern recognition are clarified firstly. The prerequisite to realize the online recognition is proposed and recognition rules for partial flow pattern are obtained based on the massive experimental data. The standard templates for every flow regime feature are calculated with self-organization cluster algorithm. The multi-sensor data fusion method is proposed to realize the online recognition of multiphase flow regime with the pressure and differential pressure signals, which overcomes the severe influence of fluid flow velocity and the oil fraction on the recognition. The online recognition method is tested in the practice, which has less than 10 percent measurement error. The method takes advantages of high confidence, good fault tolerance and less requirement of single sensor performance.

  20. Regimes de espaço

    Directory of Open Access Journals (Sweden)

    Eric Landowski

    2015-06-01

    Full Text Available Space Regimes - Based on a general model of interaction, the present analysis attempts to account for the diversity of the ways space may be apprehended in lived experience. It leads to the definition of four configurations that correspond to as many regimes of relation with the environing world. The conventional space of the circulation of values takes the archetypical shape of the net (incarnated today by Internet. The operational space is that of our dealing with objects within a material environment seen as a tissue of stable and intelligible relations. The experienced space of bodies’ movement, to which one may give as an emblem the spiral, expresses the dynamics of sensitive relationships between the self and the other. The existential space is that of our presence in a universe without boundaries, which no one can properly represent oneself but that nonetheless haunts art and thought: its figure is the abyss.

  1. Deuteron structure in the deep inelastic regime

    CERN Document Server

    Canal, C A Garcìa; Vento, V

    2016-01-01

    We study nuclear effects on the deuteron in the deep inelastic regime using the newest available data analyzing their $Q^2$ dependence. We conclude that precise EMC ratios for large $Q^2$ (> 30, GeV$^2$) cannot be obtained without considering these nuclear effects. For this purpose we use a scheme which parametrizes these effects in a simple manner and compare our results with other recent proposals.

  2. The Forex Regime and EMU Expansion

    OpenAIRE

    Foreest, P.W.; de Vries, Casper

    2002-01-01

    This paper provides evidence that the choice of the foreign exchange regime is not of first order importance for achieving high output growth. It is argued that due to the forward looking nature of the foreign exchange market, exchange rate stability hinges on the current and anticipated coherency of monetary and fiscal policies. We demonstrate this empirically on a panel including potential EMU accession countries. By means of rank regression analysis we uncover the partial links across the ...

  3. Dynamic Regime of Ignition of Solid Propellant

    Directory of Open Access Journals (Sweden)

    Zolotorev Nikolay

    2016-01-01

    Full Text Available This article presents a dynamic regime of exposure of the radiant flux on the sample of gun-cotton. Obtained time the ignition of gun-cotton in the heating conditions of increasing heat flux in the range from 0.2 W/cm2 to 22 W/cm2. A comparison of the delay times of the ignition when heated variable and constant heat flux.

  4. Exchange Rate Regimes and International Business Cycles

    OpenAIRE

    Theptida Sopraseuth

    2003-01-01

    This paper investigates the impact of exchange rate regimes on international business cycles and focuses on the consequences of membership to the European Monetary System. The volatility puzzle uncovered by Baxter and Stockman [1989, Journal of Monetary Economics 23, 377–401] after assessing the consequences of the Bretton Woods system turns out to be a robust stylized fact: real and nominal exchange rates display a higher volatility under floating rates while the variability of macroeconom...

  5. APPLICABLE LAW PRINCIPLES FOR MATRIMONIAL PROPERTY REGIMES

    OpenAIRE

    Andreea-Lorena CODREANU

    2014-01-01

    More and more often, marriages are concluded between people of different nationalities, which brings an extraneous dimension to the legal status and matrimonial property regime jurisdiction conflict. Many European states (among which Romania, through its new Civil Code) offer the advantage of clauses that could be used by any of the spouses, with regard to the applicable law. In case of divorce, consequences differ with the applicable law. Spouses who divorce and divide their assets based on ...

  6. Monetary policy regimes and the Nordic model

    OpenAIRE

    Schewe, Theo

    2015-01-01

    In many contexts, the Nordic countries are regarded as a natural bloc of nations that have common political, cultural and economic characteristics. Open to globalisation, the small Nordic countries are exposed to strong global competition and cyclical influences. Therefore, the basis of the model may be to combine collective risk sharing and openness to globalisation. Studies of the Nordic model do not focus on the question whether the monetary policy regime is a constitutive element of the m...

  7. Financing welfare regimes: mapping heterogeneous revenue structures

    OpenAIRE

    Gough, Ian; Abu-Sharkh, Miriam

    2011-01-01

    This article studies how the composition of public revenues in terms of sources (such as taxation, social insurance contributions, mineral rents, foreign aid) is associated with different welfare regimes and social policy outcomes. It is divided into two halves: a review of literature and research, and a cross-national data analysis. The first half reviews literature on the emergence of tax and revenue systems in the West, and on the relevance of these frameworks and findings to developing co...

  8. Snowpack regimes of the Western United States

    OpenAIRE

    Trujillo, Ernesto; Molotch, Noah P.

    2014-01-01

    Snow accumulation and melt patterns play a significant role in the water, energy, carbon, and nutrient cycles in the montane environments of the Western United States. Recent studies have illustrated that changes in the snow/rainfall apportionments and snow accumulation and melt patterns may occur as a consequence of changes in climate in the region. In order to understand how these changes may affect the snow regimes of the region, the current characteristics of the snow accumulation and mel...

  9. Tax incentive regimes: models and research methods

    OpenAIRE

    Sokolovska, Olena; Sokolovskyi, Dmytro

    2014-01-01

    Paper deals with problem of effectiveness of tax incentive regimes. Support of priority industries remains currently important for transition economies. At once, such countries more often than developed ones face the imperfection of tax incentive mechanisms. Notably, such problems are common to Ukrainian and Moldavian economies, where the legislative support or its implementation in the area of preferential taxation of innovative enterprises which contribute essentially to sustainable develop...

  10. Macro Regime and Economic Growth in China

    OpenAIRE

    Yuwen Dai

    2007-01-01

    In this paper, we investigate the relationship between Chinese macroeconomic policy and economic growth, and examine how the choice of macroeconomic regime affects economic performance in China. An open-economy model is developed for this purpose. It is a three-sector “almost small" open-economy macroeconomic model, with asset markets and forward-looking agents. This open-economy model is then adopted to analyse the implications of both domestic and external growth shocks to the Chinese econo...

  11. Exchange Rate Regimes, Location, and Specialization

    OpenAIRE

    Luca Antonio Ricci

    2006-01-01

    This paper investigates the effects of fixed versus flexible exchange rates on firms' location choices and on countries' specialization patterns. In a two-country, twodifferentiated-goods monetary model, uncertainty arises after wages are set and prices are optimally chosen. The paper shows that countries are more specialized under flexible than fixed rates, which indicates that the pattern of specialization is not uniquely defined by trade models but also depends on the exchange rate regime....

  12. British Columbia's new coalbed methane royalty regime

    International Nuclear Information System (INIS)

    The British Columbia Ministry of Energy and Mines is promoting the development of the coalbed methane (CBM) industry in the province in order to make CBM a viable and competitive investment option for industry. It is establishing a regulatory and fiscal regime for CBM development. Issues of concern regarding CBM development include water production, gas production rates, well numbers, and marginal economics. The features of the CBM royalty regime include a new producer cost of service allowance, the creation of a CBM royalty tax bank to collect excess PCOS allowances, and a royalty tax credit for wells drilled by the end of February, 2004. The marginal well adjustment factor threshold has been raised from 180 mcf per day to 600 mcf per day for CBM only. It was noted that royalties will probably not be payable for several years following the first commercial well because royalties are very depending on capital and operating costs, local infrastructure and price. Royalty regimes cannot save CBM from low gas prices, poor resources or economics. 2 figs

  13. Constructing an interdisciplinary flow regime recommendation

    Science.gov (United States)

    Bartholow, J.M.

    2010-01-01

    It is generally agreed that river rehabilitation most often relies on restoring a more natural flow regime, but credibly defining the desired regime can be problematic. I combined four distinct methods to develop and refine month-by-month and event-based flow recommendations to protect and partially restore the ecological integrity of the Cache la Poudre River through Fort Collins, Colorado. A statistical hydrologic approach was used to summarize the river's natural flow regime and set provisional monthly flow targets at levels that were historically exceeded 75% of the time. These preliminary monthly targets were supplemented using results from three Poudre-specific disciplinary studies. A substrate maintenance flow model was used to better define the high flows needed to flush accumulated sediment from the river's channel and help sustain the riparian zone in this snowmelt-dominated river. A hydraulic/habitat model and a water temperature model were both used to better define the minimum flows necessary to maintain a thriving cool water fishery. The result is a range of recommended monthly flows and daily flow guidance illustrating the advantage of combining a wide range of available disciplinary information, supplemented by judgment based on ecological principles and a general understanding of river ecosystems, in a highly altered, working river. ?? 2010 American Water Resources Association.

  14. The kinetic regime of the Vicsek model

    Science.gov (United States)

    Chepizhko, A. A.; Kulinskii, V. L.

    2009-12-01

    We consider the dynamics of the system of self-propelling particles modeled via the Vicsek algorithm in continuum time limit. It is shown that the alignment process for the velocities can be subdivided into two regimes: "fast" kinetic and "slow" hydrodynamic ones. In fast kinetic regime the alignment of the particle velocity to the local neighborhood takes place with characteristic relaxation time. So, that the bigger regions arise with the velocity alignment. These regions align their velocities thus giving rise to hydrodynamic regime of the dynamics. We propose the mean-field-like approach in which we take into account the correlations between density and velocity. The comparison of the theoretical predictions with the numerical simulations is given. The relation between Vicsek model in the zero velocity limit and the Kuramoto model is stated. The mean-field approach accounting for the dynamic change of the neighborhood is proposed. The nature of the discontinuity of the dependence of the order parameter in case of vectorial noise revealed in Gregorie and Chaite, Phys. Rev. Lett., 92, 025702 (2004) is discussed and the explanation of it is proposed.

  15. Autonomia e relevância dos regimes The autonomy and relevance of regimes

    Directory of Open Access Journals (Sweden)

    Gustavo Seignemartin de Carvalho

    2005-12-01

    Full Text Available Teorias institucionalistas na disciplina de relações internacionais usualmente definem regimes como um conjunto de normas e regras formais ou informais que permitem a convergência de expectativas ou a padronização do comportamento de seus participantes em uma determinada área de interesses com o objetivo de resolver problemas de coordenação que tenderiam a resultados não pareto-eficientes. Como estas definições baseadas meramente na "eficiência" dos regimes não parecem suficientes para explicar sua efetividade, o presente artigo propõe uma definição diferente para regimes: a de arranjos políticos que permitem a redistribuição dos ganhos da cooperação pelos participantes em uma determinada área de interesses em um contexto de interdependência. Regimes possuiriam efetividade pela sua autonomia e relevância, ou seja, por possuírem existência objetiva autônoma da de seus participantes e por influenciarem seu comportamento e expectativas de maneiras que não podem ser reduzidas à ação individual de nenhum deles. O artigo inicia-se com uma breve discussão sobre as dificuldades terminológicas associadas ao estudo de regimes e a definição dos conceitos de autonomia e relevância. Em seguida, classifica os diversos autores participantes do debate em duas perspectivas distintas, uma que nega (não-autonomistas e outra que atribui (autonomistas aos regimes autonomia e relevância, e faz uma breve análise dos autores e tradições mais significativos para o debate, aprofundando-se nos autonomistas e nos argumentos que reforçam a hipótese aqui apresentada. Ao final, o artigo propõe uma decomposição analítica dos regimes nos quatro elementos principais que lhes propiciam autonomia e relevância: normatividade, atores, especificidade da área de interesses e interdependência complexa com o contexto.Regimes are defined by institutionalist theories in the discipline of International Relations as formal or informal sets

  16. 尿激酶联合依达拉奉治疗急性脑梗死的临床观察%Efficacy Observation of Urokinase Combined with Edaravone in the Treatment of Acute Cerebral Infarction

    Institute of Scientific and Technical Information of China (English)

    王宪州

    2011-01-01

    目的 观察尿激酶与依达拉奉联合治疗急性脑梗死的临床疗效.方法 将我院2008年3月-2010年3月期间收治的52例急性脑梗死患者随机均分为观察组和对照组.2组患者经明确诊断后均给予依达拉奉(30 mg,bid)治疗,观察组在对照组的基础上加用尿激酶(20万U,qd)联合治疗,治疗前后比较2组的疗效、日常生活能力评分(ADL)及神经功能缺损评分(ESS).结果 治疗后与对照组比较,观察组痊愈率和总有效率以及治疗后的ADL评分均明显提高,而治疗后的ESS评分则明显降低,2组比较差异均具有统计学意义(P<0.05).2组均未见严重不良反应.结论 尿激酶与依达拉奉联合治疗急性脑梗死,其疗效确切,且不良反应少.%OBJECTIVE: To probe into the clinical efficacy of urokinase combined with edaravone in the treatment of acute cerebral infarction. METHODS: 52 patients with acute cerebral infarction were selected from our hospital during Mar. 2008 - Mar. 2010. Those patients were randomly divided into observation group and control group. 2 groups were given edaravone(30 mg.bid), and observation group was additionally given urokinase(200 000 U,qd). The efficacy ADL and ESS were compared before and after treatment. RESULTS: Compared with control group, the cure rate, total effective rate and the activity of daily living scores in observation group were significantly unproved while neurological deficit scores after treatment were significantly lowered, there were statistically significant difference (P<0.05). There was no serious ADRs found in both groups. CONCLUSION: Urokinase combined with edaravone have remarkable curative effect on acute cerebral infarction. The ADRs is low.

  17. Refrigeration. Two-Phase Flow. Flow Regimes and Pressure Drop

    DEFF Research Database (Denmark)

    Knudsen, Hans-Jørgen Høgaard

    2002-01-01

    The note gives the basic definitions used in two-phase flow. Flow regimes and flow regimes map are introduced. The different contributions to the pressure drop are stated together with an imperical correlation from the litterature....

  18. Asymptotic Regime in N Random Interacting Species

    CERN Document Server

    Fiasconaro, A; Valenti, D

    2005-01-01

    The asymptotic regime of a complex ecosystem with N random interacting species and in the presence of an external multiplicative noise is analyzed. We find the role of the external noise on the long time probability distribution of the i_th density species, the extinction of species and the local field acting on the i_th population. We analyze in detail the transient dynamics of this field and the cavity field, which is the field acting on the i_th species when this is absent. We find that the presence or the absence of some population give different asymptotic distributions of these fields.

  19. REVIEW OF REGIME THEORY OF ALLUVIAL CHANNELS

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    One of the most important problems in river engineering is to determine a stable cross section geomenry and slope for an alluvial channel. This has been the subject of considerable research for about a century and continues to be of great practical interest. Lgnoring plan geometry, an alluvi-al channel can adjust its slope, depth and width, to develop a dynamic stable condition in which it can transport a certain a-mount of water and sediment. The brief history of regime the-ory and its new development are reviewed in this paper.

  20. Muscle's Motion in an Overdamped Regime

    Institute of Scientific and Technical Information of China (English)

    AI Bao-Quan; WANG Xian-Ju; LIU Liang-Gang; M. Nakano; H. Matsuura

    2002-01-01

    Based on the stochastic inclined rods model proposed by H. Matsuura et al., we study the motion of actin myosin system in an overdamped regime. Our model is composed ofan inclined spring (rod), a myosin head and a myosin filament. The results of calculation show that the modelcan convert the random motion to one-directional motion, and the myosin head works as a resonator of random noise, which absorbs the energy through a stochastic resonance. The results show that the inclined rod and the intermolecular potential are very important for the system to move.

  1. Autoritarismus in Peru : das Fujimori-Regime

    OpenAIRE

    Crabtree, John

    2007-01-01

    The ten-years presidency of Alberto Fujimori in Peru is a classic example of the sort of hybrid democracy that emerged in various Latin American countries in the 1990s, combining a mixture of democratic and clearly non-democratic elements. Fujimori was one of the more autocratic Latin American leaders of the 1990s, he is being the only regime formally to break with pre-existing constitutional rules. Closely tied to the military and intelligence apparatus, his government involved a concentrati...

  2. Efficiency of Rectification: Reversible vs. Irreversible Regimes

    Science.gov (United States)

    Sokolov, I. M.

    2002-11-01

    Both man-made locomotive devices and molecular motors use gears to transform a reciprocating motion into a directed one. One of the most common gears is a rectifier, a mechanically irreversible appliance. The maximal energetic efficiency of an isothermic gear is bounded by unity, as a consequence of the Second Law. However, approaching this ideal efficiency does not imply approaching reversibility. We discuss what properties of a rectifier mostly influence the transduction efficiency and show that an appliance which locks under backward force is just the one which can approach the ideal efficiency either in the reversible or in the irreversible regime.

  3. The Tributary Regime in the oil sector

    International Nuclear Information System (INIS)

    The tributary regime of the oil sector, is framed by the fiscal crisis that the country, maxim if one keeps in mind the importance of the fiscal revenues originated in the exploitation of these resources in Colombia, so much for the tribute coming from the foreign investment of the sector, like for the utilities generated by ECOPETROL and its impact in the public finances. However in front of this focus, the strategic importance of maintaining the investment in hydrocarbons and the paper that the fiscal politics and the tributary politics for the sector should play in the future and they constitute the government's key pieces

  4. Random nanolasing in the Anderson localized regime

    DEFF Research Database (Denmark)

    Liu, Jin; Garcia, P. D.; Ek, Sara;

    2014-01-01

    multiple scattering. The applicability of random lasers has been limited due to multidirectional emission, lack of tunability, and strong mode competition with chaotic fluctuations due to a weak mode confinement. The regime of Anderson localization of light has been proposed for obtaining stable multimode...... random lasing, and initial work concerned macroscopic one-dimensional layered media. Here, we demonstrate on-chip random nanolasers where the cavity feedback is provided by the intrinsic disorder. The strong confinement achieved by Anderson localization reduces the spatial overlap between lasing modes...

  5. Asian Aspirations for Climate Regime Beyond 2012

    International Nuclear Information System (INIS)

    This report is based on the second round of 'The Asia-Pacific Consultations on Climate Regime Beyond 2012' in 2006 to further advance the frank exchange of opinions of policy makers, academics, and the private sector on specific issues of high priority to the region. These include: energy security and development, Clean Development Mechanism (CDM), technology development and transfer, and adaptation. This report presents a summary of what has been learnt through the current consultations, interviews, and questionnaire surveys with policy makers and climate policy researchers across the region

  6. Langmuir wave filamentation in the kinetic regime

    CERN Document Server

    Silantyev, Denis A; Rose, Harvey A

    2016-01-01

    Nonlinear Langmuir wave in the kinetic regime $k\\lambda_D\\gtrsim0.2$ has a transverse instability, where $k$ is the wavenumber and $\\lambda_D$ is the Debye length. The nonlinear stage of that instability development leads to the filamentation of Langmuir waves. Here we study the linear stage of transverse instability of both Bernstein-Greene-Kruskal (BGK) modes and dynamically prepared BGK-like initial conditions to find the same instability growth rate suggesting the universal mechanism for the kinetic saturation of stimulated Raman scatter in laser-plasma interaction experiments. Multidimensional Vlasov simulations results are compared to the theoretical predictions.

  7. Reconfigurable photoinduced metamaterials in the microwave regime

    CERN Document Server

    Rizza, Carlo; De Paulis, Francesco; Palange, Elia; Orlandi, Antonio; Columbo, Lorenzo; Prati, Franco

    2014-01-01

    We investigate optically reconfigurable dielectric metamaterials at gigahertz frequencies. More precisely, we study the microwave response of a subwavelength grating optically imprinted into a semiconductor slab. In the homogenized regime, we analytically evaluate the ordinary and extraordinary component of the effective permittivity tensor by taking into account the photo-carrier dynamics described by the ambipolar diffusion equation. We analyze the impact of semiconductor parameters on the gigahertz metamaterial response which turns out to be highly reconfigurable by varying the photogenerated grating and which can show a marked anisotropic behavior.

  8. The design of environmental regimes: Social construction, contextuality, and improvisation

    OpenAIRE

    Lejano

    2006-01-01

    While much of the literature on environmental regimes has focused on effectiveness, this article takes a new look at a lesser-studied topic, the evolution of regime design. Understanding how regimes differ in design, and how various factors and processes shape such design, is important if we are to more carefully craft these regimes. We should also pay close attention to the formative role of social construction and context. Focusing on transboundary marine programs, we see that their designs...

  9. Low-frequency regime transitions and predictability of regimes in a barotropic model

    CERN Document Server

    Nadiga, B T

    2016-01-01

    Predictability of flow is examined in a barotropic vorticity model that admits low frequency regime transitions between zonal and dipolar states. Such transitions in the model were first studied by Bouchet and Simonnet (2009) and are reminiscent of regime change phenomena in the weather and climate systems wherein extreme and abrupt qualitative changes occur, seemingly randomly, after long periods of apparent stability. Mechanisms underlying regime transitions in the model are not well understood yet. From the point of view of atmospheric and oceanic dynamics, a novel aspect of the model is the lack of any source of background gradient of potential-vorticity such as topography or planetary gradient of rotation rate (e.g., as in Charney & DeVore '79). We consider perturbations that are embedded onto the system's chaotic attractor under the full nonlinear dynamics as bred vectors---nonlinear generalizations of the leading (backward) Lyapunov vector. We find that ensemble predictions that use bred vector per...

  10. Hard and Soft Excitation Regimes of Kerr Frequency Combs

    CERN Document Server

    Matsko, Andrey B; Ilchenko, Vladimir S; Seidel, David; Maleki, Lute

    2011-01-01

    We theoretically study the stability conditions and excitation regimes of hyper-parametric oscillation and Kerr frequency comb generation in continuously pumped nonlinear optical resonators possessing anomalous group velocity dispersion. We show that both hard and soft excitation regimes are possible in the resonators. Selection between the regimes is achieved via change in the parameters of the pumping light.

  11. Level-ARCH Short Rate Models with Regime Switching

    DEFF Research Database (Denmark)

    Christiansen, Charlotte

    This paper introduces regime switching volatility into level- ARCH models for the short rates of the US, the UK, and Germany. Once regime switching and level effects are included there are no gains from including ARCH effects. It is of secondary importance exactly how the regime switching...

  12. Change of secondary water regime of Paks NPP. Change of secondary water regime at Unit 2

    International Nuclear Information System (INIS)

    The installation of high pH water regime during the 17th cycle of Unit 2 aimed to decrease the amount of transportation inlet of erosion-corrosion products (magnitude) in feedwater to SGs. The resolution of OAH-NBI permitting the installation ordained to make an evaluation of the process. The main conclusions and results are discussed. The high pH water regime proved to be adequate in the case of Unit 2 as well, similarly to Units 3, 4 and 1. (R.P.)

  13. Clinical application on urokinase, papaverine angiography in diabetic foot%尿激酶、罂粟碱在糖尿病足血管造影中的临床应用

    Institute of Scientific and Technical Information of China (English)

    任毅; 陈君辉; 贺平

    2012-01-01

    To discuss urokinase, papaverine angiography in diabetic foot in clinical application. Methods Forty-seven cases of vascular doppler examination of lower extremity arterial stenosis prompted the merger and the performance of lower limb ischemia in diabetic patients, after the DSA angiography ,nineteen cases who had tibial, peroneal artery, the distal or foot small artery stenosis or occlusion , used transcatheter bolus infusion method using 25 - 50Uten-thousand units of urokinase, papaverine 30 ~60mg. Results Fifteen cases of transcatheter infusion of urokinase, papaverine had marked improvement in the performance of lower limb ischemia, and through the comprehensive medical treatment, target vessel around the collateral vessels increased significantly in the total group of 100 percent of cases, stenosis lumen expansion over the previous was 68% , re-opening of vascular occlusion accounted for 36. 8%. 0 — Ⅱ level surface of foot ulcer disease healed , Ⅱ ~ Ⅳ grade of foot ulcer surface drying, shrinking around the inflammation subsided. Conclusion Intra-arterial infusion of urokinase, papaverine can to some extent to improve lower limb blood circulation, so as to achieve remission in patients with lower extremity pain, numbness and other symptoms , has short-term efficacy, no heroatoma, vascular rupture, distal embolism and other vascular complications occurred.%目的 探讨尿激酶、罂粟碱在糖尿病足血管造影中的临床应用.方法 对47例血管多普勒检查提示下肢动脉狭窄且合并下肢缺血表现糖尿病患者,行DSA血管造影后,对其中19例胫、腓动脉中、远段或足部微小动脉狭窄或闭塞患者,经导管采用团注法灌注尿激酶25~ 50U、罂粟碱30~60mg行溶栓,扩血管治疗.结果 19例经导管灌注尿激酶、罂粟碱患者下肢缺血表现明显改善,并通过内科综合治疗,靶血管周围侧支血管明显增多占全组病例的100%,狭窄段血管腔较前扩张占68%,

  14. The Asymptotic Regime of High Density QCD

    CERN Document Server

    Gay-Ducati, M B

    2000-01-01

    We discuss the distinct approaches for high density QCD (hdQCD) in the asymptotic regime of large values of parton density. We derive the AGL equation for running coupling constant and obtain the asymptotic solution, demonstrating that the property of partial saturation of the solution of the AGL equation is not modified by the running of the coupling constant. We show that in this kinematical regime, the solution of the AGL equation coincides with the solution of an evolution equation, obtained recently using the McLerran-Venugopalan approach. Using the asymptotic behavior of the gluon distribution we calculate the $F_2$ structure function assuming first that the leading twist relation between these two quantities is valid and second that this relation is modified by the higher twist terms associated to the unitarity corrections. In the first case we obtain that the corresponding $F_2$ structure function is linearly proportional to $ln s$, which agrees with the results obtained recently by Kovchegov using a ...

  15. Dynamic regimes of random fuzzy logic networks

    Science.gov (United States)

    Wittmann, Dominik M.; Theis, Fabian J.

    2011-01-01

    Random multistate networks, generalizations of the Boolean Kauffman networks, are generic models for complex systems of interacting agents. Depending on their mean connectivity, these networks exhibit ordered as well as chaotic behavior with a critical boundary separating both regimes. Typically, the nodes of these networks are assigned single discrete states. Here, we describe nodes by fuzzy numbers, i.e. vectors of degree-of-membership (DOM) functions specifying the degree to which the nodes are in each of their discrete states. This allows our models to deal with imprecision and uncertainties. Compatible update rules are constructed by expressing the update rules of the multistate network in terms of Boolean operators and generalizing them to fuzzy logic (FL) operators. The standard choice for these generalizations is the Gödel FL, where AND and OR are replaced by the minimum and maximum of two DOMs, respectively. In mean-field approximations we are able to analytically describe the percolation and asymptotic distribution of DOMs in random Gödel FL networks. This allows us to characterize the different dynamic regimes of random multistate networks in terms of FL. In a low-dimensional example, we provide explicit computations and validate our mean-field results by showing that they agree well with network simulations.

  16. Variety of synchronous regimes in neuronal ensembles

    Science.gov (United States)

    Komarov, M. A.; Osipov, G. V.; Suykens, J. A. K.

    2008-09-01

    We consider a Hodgkin-Huxley-type model of oscillatory activity in neurons of the snail Helix pomatia. This model has a distinctive feature: It demonstrates multistability in oscillatory and silent modes that is typical for the thalamocortical neurons. A single neuron cell can demonstrate a variety of oscillatory activity: Regular and chaotic spiking and bursting behavior. We study collective phenomena in small and large arrays of nonidentical cells coupled by models of electrical and chemical synapses. Two single elements coupled by electrical coupling show different types of synchronous behavior, in particular in-phase and antiphase synchronous regimes. In an ensemble of three inhibitory synaptically coupled elements, the phenomenon of sequential synchronous dynamics is observed. We study the synchronization phenomena in the chain of nonidentical neurons at different oscillatory behavior coupled with electrical and chemical synapses. Various regimes of phase synchronization are observed: (i) Synchronous regular and chaotic spiking; (ii) synchronous regular and chaotic bursting; and (iii) synchronous regular and chaotic bursting with different numbers of spikes inside the bursts. We detect and study the effect of collective synchronous burst generation due to the cluster formation and the oscillatory death.

  17. Regimes of piezoelectric transformer operation II

    International Nuclear Information System (INIS)

    Piezoelectric transformers (PTs) have some attractive features for using them in power supply systems such as high efficiency and high power density, a high level of isolation between primary and secondary sides, a flat design which is well suited for a lot of applications and a low level of electromagnetic noise, just to name a few. For any application it is of utmost importance to know the behavior of a piezoelectric transformer under different conditions, which we call regimes of transformer operation. Based on this knowledge, criteria for safe operation of the PT in the power supply system and hence for the whole system as well can be derived. After having introduced the regimes of piezoelectric transformer operation in a first article on this topic in 2006 [1], in this paper we will present the derivation of important parameters based on measurements by way of an example of a symmetric PT. These measurements facilitate the basic understanding for choosing the appropriate transformer parameters necessary for its optimal as well as safe and controlled operation in power supply systems

  18. Characterizing multistationarity regimes in biochemical reaction networks.

    Directory of Open Access Journals (Sweden)

    Irene Otero-Muras

    Full Text Available Switch like responses appear as common strategies in the regulation of cellular systems. Here we present a method to characterize bistable regimes in biochemical reaction networks that can be of use to both direct and reverse engineering of biological switches. In the design of a synthetic biological switch, it is important to study the capability for bistability of the underlying biochemical network structure. Chemical Reaction Network Theory (CRNT may help at this level to decide whether a given network has the capacity for multiple positive equilibria, based on their structural properties. However, in order to build a working switch, we also need to ensure that the bistability property is robust, by studying the conditions leading to the existence of two different steady states. In the reverse engineering of biological switches, knowledge collected about the bistable regimes of the underlying potential model structures can contribute at the model identification stage to a drastic reduction of the feasible region in the parameter space of search. In this work, we make use and extend previous results of the CRNT, aiming not only to discriminate whether a biochemical reaction network can exhibit multiple steady states, but also to determine the regions within the whole space of parameters capable of producing multistationarity. To that purpose we present and justify a condition on the parameters of biochemical networks for the appearance of multistationarity, and propose an efficient and reliable computational method to check its satisfaction through the parameter space.

  19. Elastic regimes of subisostatic athermal fiber networks.

    Science.gov (United States)

    Licup, A J; Sharma, A; MacKintosh, F C

    2016-01-01

    Athermal models of disordered fibrous networks are highly useful for studying the mechanics of elastic networks composed of stiff biopolymers. The underlying network architecture is a key aspect that can affect the elastic properties of these systems, which include rich linear and nonlinear elasticity. Existing computational approaches have focused on both lattice-based and off-lattice networks obtained from the random placement of rods. It is not obvious, a priori, whether the two architectures have fundamentally similar or different mechanics. If they are different, it is not clear which of these represents a better model for biological networks. Here, we show that both approaches are essentially equivalent for the same network connectivity, provided the networks are subisostatic with respect to central force interactions. Moreover, for a given subisostatic connectivity, we even find that lattice-based networks in both two and three dimensions exhibit nearly identical nonlinear elastic response. We provide a description of the linear mechanics for both architectures in terms of a scaling function. We also show that the nonlinear regime is dominated by fiber bending and that stiffening originates from the stabilization of subisostatic networks by stress. We propose a generalized relation for this regime in terms of the self-generated normal stresses that develop under deformation. Different network architectures have different susceptibilities to the normal stress but essentially exhibit the same nonlinear mechanics. Such a stiffening mechanism has been shown to successfully capture the nonlinear mechanics of collagen networks. PMID:26871101

  20. Efficient expression of recombinant human pro-urokinase in CHO cells and its purification%重组人尿激酶原在CHO细胞中的高效表达及其纯化

    Institute of Scientific and Technical Information of China (English)

    李世崇; 叶玲玲; 刘红; 张正光; 高丽华; 胡显文; 陈昭烈

    2012-01-01

    The efficient expression vector for recombinant human pro-urokinase (rhPro-UK) was constructed with the combination of chicken β-globin MAR sequences, human elongation factor alphal ( hEF-1α) housekeeping gene regulatory sequences, and RNA stability and the output sequence. The rhPro-UK expression level reached 1299 IU/cells6/d in CHO ( Chinese hamster ovary) cell lines. The rhPro-UK containing supernatant purified with cation exchange chromatography, hydrophobic interaction chromatog-raphy, gel exclusion chromatography process resulted in rhPro-UK purity over 95% with recovery rate of 60% -70%.%用鸡β- globin的MAR序列和人看家基因延伸因子1α(hEF-1α)的调控序列以及旱獭RNA稳定与输出序列,构建了重组人尿激酶原(recombinant human pro-urokinase,rhPro - UK)的高效表达载体,在CHO细胞中获得了rhPro - UK的高效稳定表达,rhPro - UK的表达水平达到1299 IU(以百万细胞1d的表达量计).采用阳离子交换层析、疏水层析和凝胶排阻层析的三步工艺纯化表达rhPro - UK的CHO细胞培养上清液,rhPro - UK的纯度达到98%、回收率为60% ~70%.

  1. Flavonol-enriched fraction from Vaccinium macrocarpon fruit inhibits matrix metalloproteinase-2, matrix metalloproteinase-9 and urokinase-type plasminogen activator expression in human prostate cancer cells in vitro

    Directory of Open Access Journals (Sweden)

    James MacPhee

    2014-11-01

    Full Text Available Background: Prostate cancer, amongst other cancer types has a genetic and environmental component, which can contribute to prostate cancer development and progression. Vaccinum macrocarpon (American cranberry is a botanical that contains several phytochemicals which have been suggested to play a role in preventing cardiovascular disease, cancer, and urinary tract infections as well as in the maintenance of oral health. Context and purpose of this study: This investigation evaluated the effects of a flavonolenriched fraction (FL from the American cranberry (Vaccinium macrocarpon containing quercetin and myricetin glycosides on matrix metalloproteinase (MMP and urokinase-type plasminogen activator (uPA activities and their associated regulatory proteins in DU145 human prostate cancer cells in vitro. Results: A flavonol-enriched fraction (FL was prepared from Vaccinium macrocarpon berries and the effect of this fraction on prostate cancer cell behaviour was assessed using biochemical and molecular approaches including cytotoxicity assays and Western blot analysis to determine protein expression. Cranberry FL decreased cellular viability of DU145 cells at a concentration of 25 ug/ml by 20% after 6 hours of treatment. Further investigations determined that associated with this cytotoxicity, cranberry FL decreases matrix metalloproteinase (MMP ( specifically MMP-2 and MMP-9 activity and urokinase plasminogen activator (uPA activity through effects on specific temporal MMP regulators and uPA regulators and by affecting either the phosphorylation status and/or expression of specific MAP kinase, PI-3 kinase, NF-kB and AP-1 pathway associated proteins. Conclusion: This study demonstrates, for the first time, the ability of Vaccinium macrocarpon flavonols to modulate cellular pathways associated with migration, invasion, and proliferation, suggesting that cranberry (Vaccinium macrocarpon is a viable candidate for further research as a natural product that

  2. Essence and Prospect of International Export Control Regimes

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ International export control regimes are referring to the regimes and systems concerning limitation of export and transference of military-use and dual-use items and related parts as well as technologies. In the past couple of years, the control regimes have shown a strengthening posture. In addition, a new global export control regime is brewing. Hence export control has caught the attention of the international community, and particularly of the circles of armament control and commerce. The author would like to make some analyses of the posture and then try to predict the trend of international export control regimes.

  3. Quaternary Faults and Stress Regime of Venezuela

    Directory of Open Access Journals (Sweden)

    F.A. Audemard M.

    2006-12-01

    Full Text Available Spatial configuration of Quaternary active tectonic features along the southern Caribbean plate boundary suggests that the region is subject to a compressive strike-slip (transpressional senso lato regime, characterized by a NNW-SSE maximum horizontal stress (sH=s1 and/or an ENE-WSW minimum (s h=s3 or s2 horizontal stress. Stress inversion applied to fault-plane kinematic indicators measured essentially in Plio-Quaternary sedimentary rocks confirms this tectonic regime. Accordingly, this stress regime is responsible for the Quaternary activity and kinematics of six sets of brittle features along northern Venezuela (from Colombia in the west to Trinidad in the east: (1 east-west rightlateral faults, (2 NW right-lateral faults -acting as synthetic Riedel shears-, (3 ENE to east-west dextral faults -P shears-, (4 NNW normal faults, (5 almost north-south left-lateral faults -antithetic Riedel shears- and (6 mostly subsurface ENE reverse faults associated with folding of the same orientation. Brittle deformation conforms to the simple shear model, although not all the deformation can be accounted for it since strain partitioning is also taking place because regional folding and thrusting are due to the normal-to-structure component of the relative slip vector between the Caribbean and South America plates. On the other hand, the maximum horizontal stress in western Venezuela, particularly in the Maracaibo block and south of the Oca-Ancón fault, progressively turns counter-clockwise to become more east-west oriented, producing left- and right-lateral slip along the north-south striking and NE-SW striking faults, respectively. The orientation and spatial variation of this regional stress field in western Venezuela results from the superposition of the two major neighboring interplate maximum horizontal stress orientations (sH: roughly east-west trending stress across the Nazca-South America type-B subduction along the pacific coast of Colombia and NNW

  4. Institutional design and regime effectiveness in transboundary river management – the Elbe water quality regime

    Directory of Open Access Journals (Sweden)

    I. Dombrowsky

    2007-06-01

    Full Text Available The literature on transboundary river management suggests that institutions play an important role in bringing cooperation about. However, the knowledge on how they should be designed in order to do so remains limited. One way to learn more about adequate institutional design is to assess the effectiveness of existing regimes, and to trace the causal relationships leading to the respective outcomes. In order to gain further insights into the relationship of institutional design and regime effectiveness, this paper presents a study on the water quality regime of the International Commission for the Protection of the Elbe (ICPE. The analysis is based on a review of pertinent documents and ten qualitative interviews with Czech and German Commission members and NGO representatives. Particular emphasis has been put on determining the ICPE's specific contribution and the no-regime counterfactual as well as on the perceived expedience of the institutional arrangements. The study shows that overall due to external as well as internal institutional factors the ICPE proved relatively successful, and as such it also provides insights into how institutions matter: The commission served as platform for joint problem solving by identifying priorities for action. These international obligations increased the power of national administrations and their access to funds. At the same time, the Commission's reporting to the public served as an enforcement mechanism. However, the ICPE's contribution towards achieving the various goals varied significantly between the different areas of activity. It was high where the main responsibility for action was with the public authorities, such as in the area of wastewater treatment and the establishment of an international alarm plan and model. It was practically non existent in the reduction of non-point pollution from agriculture, where the success depended on the behavior of individual private actors (farmers. From a

  5. Two regimes of forced turbulent convection

    CERN Document Server

    Eidelman, A; Kleeorin, N; Rogachevskii, I; Sapir-Katiraie, I

    2009-01-01

    We study experimentally a forced turbulent convection in the Rayleigh-B\\'{e}nard apparatus with an additional source of turbulence produced by the two oscillating grids located nearby the side walls of the chamber. Two different regimes have been observed in the forced turbulent convection. When the frequency of the grid oscillations f>2 Hz, the large-scale circulation (LSC) is totally destroyed, and the destruction of the LSC is accompanied by a strong change of the mean temperature distribution. For the very low frequency the thermal structure inside the LSC is inhomogeneous and anisotropic. The hot thermal plumes accumulate at one side of LSC, and cold plumes concentrate at the opposite side of LSC. The mean temperature gradient in the horizontal direction inside the LSC is significantly larger than in the vertical direction. For the high frequency (f > 10 Hz), LSC has not been observed and the mean temperature gradient in the central flow region in the vertical direction, \

  6. The Great Lakes' regional climate regimes

    Science.gov (United States)

    Sugiyama, Noriyuki

    For the last couple of decades, the Great Lakes have undergone rapid surface warming. In particular, the magnitude of the summer surface-warming trends of the Great Lakes have been much greater than those of surrounding land (Austin and Colman, 2007). Among the Great Lakes, the deepest Lake Superior exhibited the strongest warming trend in its annual, as well as summer surface water temperature. We find that many aspects of this behavior can be explained in terms of the tendency of deep lakes to exhibit multiple regimes characterized, under the same seasonally varying forcing, by the warmer and colder seasonal cycles exhibiting different amounts of wintertime lake-ice cover and corresponding changes in the summertime lake-surface temperatures. In this thesis, we address the problem of the Great Lakes' warming using one-dimensional lake modeling to interpret diverse observations of the recent lake behavior. (Abstract shortened by ProQuest.).

  7. Regime shifts in models of dryland vegetation

    CERN Document Server

    Zelnik, Yuval R; Yizhaq, Hezi; Bel, Golan; Meron, Ehud

    2013-01-01

    Drylands are pattern-forming systems showing self-organized vegetation patchiness, multiplicity of stable states and fronts separating domains of alternative stable states. Pattern dynamics, induced by droughts or disturbances, can result in desertification shifts from patterned vegetation to bare soil. Pattern-formation theory suggests various scenarios for such dynamics; an abrupt global shift involving a fast collapse to bare soil, a gradual global shift involving the expansion and coalescence of bare-soil domains, and an incipient shift to a hybrid state consisting of stationary bare-soil domains in an otherwise periodic pattern. Using models of dryland vegetation we address the question which of these scenarios can be realized. We found that the models can be split into two groups: models that exhibit multiplicity of periodic-pattern and bare-soil states, and models that exhibit, in addition, multiplicity of hybrid states. Furthermore, in all models we could not identify parameter regimes in which bare-s...

  8. Environment Flow Assessment with Flow Regime Transition

    Science.gov (United States)

    Su, J.; Ho, C. C.; Chang, L. C.

    2015-12-01

    To avoid worsen river and estuarine ecosystems cause by overusing water resources, environmental flows conservation is applied to reduce the impact of river environment. Environmental flows refer to water provided within a river, wetland or coastal zone to sustain ecosystems and benefits to human wellbeing. Environment flow assessment is now widely accepted that a naturally variable flow regime, rather than just a minimum low flow. In this study, we propose four methods, experience method, Tenant method, hydraulic method and habitat method to assess the environmental flow of base flow, flush flow and overbank flow with different discharge, frequency and occurrence period. Dahan River has been chosen as a case to demonstrate the assessment mechanism. The alternatives impact analysis of environment and human water used provides a reference for stakeholders when holding an environmental flow consultative meeting.

  9. Nonlinear regimes of forced magnetic reconnection

    Energy Technology Data Exchange (ETDEWEB)

    Vekstein, G., E-mail: g.vekstein@manchester.ac.uk [JBCA, School of Physics and Astronomy, University of Manchester, Manchester M13 9PL (United Kingdom); STEL, Nagoya University, Nagoya, Aichi 464-8601 (Japan); Kusano, K. [STEL, Nagoya University, Nagoya, Aichi 464-8601 (Japan)

    2015-09-15

    This letter presents a self-consistent description of nonlinear forced magnetic reconnection in Taylor's model of this process. If external boundary perturbation is strong enough, nonlinearity in the current sheet evolution becomes important before resistive effects come into play. This terminates the current sheet shrinking that takes place at the linear stage and brings about its nonlinear equilibrium with a finite thickness. Then, in theory, this equilibrium is destroyed by a finite plasma resistivity during the skin-time, and further reconnection proceeds in the Rutherford regime. However, realization of such a scenario is unlikely because of the plasmoid instability, which is fast enough to develop before the transition to the Rutherford phase occurs. The suggested analytical theory is entirely different from all previous studies and provides proper interpretation of the presently available numerical simulations of nonlinear forced magnetic reconnection.

  10. Different spreading regimes of spray-flames

    Science.gov (United States)

    Suard, Sylvain; Haldenwang, Pierre; Nicoli, Colette

    2004-05-01

    We present a minimal model of spray combustion to investigate a flame front propagating through a fuel-lean mixture of fuel vapor, droplets and air. The model relies on a main control parameter, Da, named the Damkoehler number, which allows us to take into account a large variety of fuel sprays. Numerical results reveal, as a function of Da, a wide range of spray-flame structures, including the classical gaseous premixed flame, a specific regime controlled by vaporisation, and a pulsating mode of propagation. The latter appears when the vaporisation is smaller than (or equal to) the reaction time, and it occurs even with a unit Lewis number. To cite this article: S. Suard et al., C. R. Mecanique 332 (2004).

  11. Fasting and 2-Hour Plasma Glucose and Insulin

    Science.gov (United States)

    Libman, Ingrid M.; Barinas-Mitchell, Emma; Bartucci, Andrea; Chaves-Gnecco, Diego; Robertson, Robert; Arslanian, Silva

    2010-01-01

    OBJECTIVE To determine whether elevated fasting or 2-h plasma glucose and/or insulin better reflects the presence of cardiovascular disease (CVD) risk markers in an overweight pediatric population with normal glucose tolerance. RESEARCH DESIGN AND METHODS A total of 151 overweight youths (8–17 years old) were evaluated with oral glucose tolerance tests and measurement of CVD risk factors. The study population was categorized according to quartiles of fasting and 2-h glucose and insulin levels. ANCOVA, adjusted for age, sex, race, Tanner stage, and percent body fat (measured by dual-energy X-ray absorptiometry), was used to compare metabolic variables between the quartiles of glucose and insulin groups. RESULTS Increasing quartiles of fasting and 2-h insulin were associated with increasing CVD risk factors. Glucose quartiles on the other hand, either fasting or at 2 h, were not. CONCLUSIONS These data suggest that hyperinsulinemia may be the earliest and/or primary metabolic alteration in childhood associated with risk markers for CVD. Prospective studies are needed. PMID:21115769

  12. The role of the media in establishing international security regimes

    Directory of Open Access Journals (Sweden)

    Chanan Naveh

    2005-04-01

    Full Text Available This article focuses primarily on the media's impact on international security regimes. It explores the ways in which the media affect the lifecycles of international regimes, from the time they are first conceived of, through their establishment, consolidation and stabilization, up until their ultimate demise. Although this paper highlights the media's role in the evolution of security regimes, it is clear that, regardless of the regime in question, media play a role throughout the lifecycle of all international regimes, whatever their character. In order to analyze the relationships of the media with security regimes in general, and specifically their contribution to each stage in their development, the article utilizes methodologies from the field of communication studies. It examines the media's agenda, "news values" and various functions, and their ability to mobilize public support for the particular issue of the regime. To date, most studies have explored the interaction between media communication patterns and global developments at the state level, or in relation to the formulation of foreign policy, while largely ignoring the international dimension of the relationship. This article attempts to remedy this situation, and the relevant processes are analysed in a case study of the anti-Iraq international security regime. It should be noted that although the paper focuses on the specific anti-Iraqi regime, it is part of a more general Anti-Rogue actors regime which includes the war against global terrorism. The study of the development of the anti-Iraq press-security regime teaches us that during international crises the media mobilize and unanimously support the regime fighting the "bad guys." But, when the regime develops and enters disputed turfs and begins to lose its legitimacy, media support diminishes, and the media may even develop into an opposing force and may join the actors fighting against this regime. Moreover, the study of

  13. Fiscal Policy and Welfare under Different Exchange Rate Regimes

    DEFF Research Database (Denmark)

    Østrup, Finn

    , and secondbecause the government's inclination to expand government spending is affectedby inflation which depends on the exchange rate regime. At low rates of inflation,the government is inclined to set a higher level of government spending under afixed exchange rate regime than under a floating exchange rate...... regime in whichthe monetary authority optimises preferences which include an employment targetand an inflation target. As government spending affects the representativeindividual's utility, the choice of exchange rate regime has an impact on welfare.Keywords: exchange rate regimes; fiscal policy......The article analyses how government spending is determined under differentexchange rate regimes in the context of a small open economy. Assumingnominal wage contracts which last for one period and assuming a benevolentgovernment which determines government spending to optimise...

  14. No interactions between previously associated 2-hour glucose gene variants and physical activity or BMI on 2-hour glucose levels

    DEFF Research Database (Denmark)

    Scott, Robert A; Chu, Audrey Y; Grarup, Niels;

    2012-01-01

    Gene-lifestyle interactions have been suggested to contribute to the development of type 2 diabetes. Glucose levels 2 h after a standard 75-g glucose challenge are used to diagnose diabetes and are associated with both genetic and lifestyle factors. However, whether these factors interact to dete...

  15. Detection and Assessment of Ecosystem Regime Shifts from Fisher Information

    OpenAIRE

    B. Roy Frieden; Christopher W. Pawlowski; Heriberto Cabezas; Arunprakash T. Karunanithi

    2008-01-01

    Ecosystem regime shifts, which are long-term system reorganizations, have profound implications for sustainability. There is a great need for indicators of regime shifts, particularly methods that are applicable to data from real systems. We have developed a form of Fisher information that measures dynamic order in complex systems. Here we propose the use of Fisher information as a means of: (1) detecting dynamic regime shifts in ecosystems, and (2) assessing the quality of the shift in terms...

  16. Spacetime-noncommutativity regime of Loop Quantum Gravity

    OpenAIRE

    AMELINO-CAMELIA, Giovanni; da Silva, Malú Maira; Ronco, Michele; Cesarini, Lorenzo; Lecian, Orchidea Maria

    2016-01-01

    A recent study by Bojowald and Paily provided a path toward the identification of an effective quantum-spacetime picture of Loop Quantum Gravity, applicable in the "Minkowski regime", the regime where the large-scale (coarse-grained) spacetime metric is flat. A pivotal role in the analysis is played by Loop-Quantum-Gravity-based modifications to the hypersurface deformation algebra, which leave a trace in the Minkowski regime. We here show that the symmetry-algebra results reported by Bojowal...

  17. Apparel exportism in Kenya: international regimes, chain governance and upgrading

    OpenAIRE

    Kindiki, M. M.

    2011-01-01

    An important question in development is how far can the contemporary global context create industrial development opportunities for the South, particularly for Low Income Countries? In an important sense, this can only be answered in non-abstract terms, since the institutional conditions facing particular industries at particular times are highly specific. In this research, a configuration of four regimes- the structural regime on production and trade in apparel, the labour regime, the neo-st...

  18. Flow regimes in a trapped vortex cell

    Science.gov (United States)

    Lasagna, D.; Iuso, G.

    2016-03-01

    This paper presents results of an experimental investigation on the flow in a trapped vortex cell, embedded into a flat plate, and interacting with a zero-pressure-gradient boundary layer. The objective of the work is to describe the flow features and elucidate some of the governing physical mechanisms, in the light of recent investigations on flow separation control using vortex cells. Hot-wire velocity measurements of the shear layer bounding the cell and of the boundary layers upstream and downstream are reported, together with spectral and correlation analyses of wall-pressure fluctuation measurements. Smoke flow visualisations provide qualitative insight into some relevant features of the internal flow, namely a large-scale flow unsteadiness and possible mechanisms driving the rotation of the vortex core. Results are presented for two very different regimes: a low-Reynolds-number case where the incoming boundary layer is laminar and its momentum thickness is small compared to the cell opening, and a moderately high-Reynolds-number case, where the incoming boundary layer is turbulent and the ratio between the momentum thickness and the opening length is significantly larger than in the first case. Implications of the present findings to flow control applications of trapped vortex cells are also discussed.

  19. Scaling regimes in spherical shell rotating convection

    CERN Document Server

    Gastine, T; Aubert, J

    2016-01-01

    Rayleigh-B\\'enard convection in rotating spherical shells can be considered as a simplified analogue of many astrophysical and geophysical fluid flows. Here, we use three-dimensional direct numerical simulations to study this physical process. We construct a dataset of more than 200 numerical models that cover a broad parameter range with Ekman numbers spanning $3\\times 10^{-7} \\leq E \\leq 10^{-1}$, Rayleigh numbers within the range $10^3 < Ra < 2\\times 10^{10}$ and a Prandtl number unity. We investigate the scaling behaviours of both local (length scales, boundary layers) and global (Nusselt and Reynolds numbers) properties across various physical regimes from onset of rotating convection to weakly-rotating convection. Close to critical, the convective flow is dominated by a triple force balance between viscosity, Coriolis force and buoyancy. For larger supercriticalities, a subset of our numerical data approaches the asymptotic diffusivity-free scaling of rotating convection $Nu\\sim Ra^{3/2}E^{2}$ in ...

  20. Multimode optomechanical system in the quantum regime

    CERN Document Server

    Nielsen, William H P; Møller, Christoffer B; Polzik, Eugene S; Schliesser, Albert

    2016-01-01

    We realise a simple and robust optomechanical system with a multitude of long-lived ($Q>10^7$) mechanical modes in a phononic-bandgap shielded membrane resonator. An optical mode of a compact Fabry-Perot resonator detects these modes' motion with a measurement rate ($96~\\mathrm{kHz}$) that exceeds the mechanical decoherence rates already at moderate cryogenic temperatures ($10\\,\\mathrm{K}$). Reaching this quantum regime entails, i.~a., quantum measurement backaction exceeding thermal forces, and thus detectable optomechanical quantum correlations. In particular, we observe ponderomotive squeezing of the output light mediated by a multitude of mechanical resonator modes, with quantum noise suppression up to -2.4 dB (-3.6 dB if corrected for detection losses) and bandwidths $\\lesssim 90\\,\\mathrm{ kHz}$. The multi-mode nature of the employed membrane and Fabry-Perot resonators lends itself to hybrid entanglement schemes involving multiple electromagnetic, mechanical, and spin degrees of freedom.

  1. Global fishery prospects under contrasting management regimes.

    Science.gov (United States)

    Costello, Christopher; Ovando, Daniel; Clavelle, Tyler; Strauss, C Kent; Hilborn, Ray; Melnychuk, Michael C; Branch, Trevor A; Gaines, Steven D; Szuwalski, Cody S; Cabral, Reniel B; Rader, Douglas N; Leland, Amanda

    2016-05-01

    Data from 4,713 fisheries worldwide, representing 78% of global reported fish catch, are analyzed to estimate the status, trends, and benefits of alternative approaches to recovering depleted fisheries. For each fishery, we estimate current biological status and forecast the impacts of contrasting management regimes on catch, profit, and biomass of fish in the sea. We estimate unique recovery targets and trajectories for each fishery, calculate the year-by-year effects of alternative recovery approaches, and model how alternative institutional reforms affect recovery outcomes. Current status is highly heterogeneous-the median fishery is in poor health (overfished, with further overfishing occurring), although 32% of fisheries are in good biological, although not necessarily economic, condition. Our business-as-usual scenario projects further divergence and continued collapse for many of the world's fisheries. Applying sound management reforms to global fisheries in our dataset could generate annual increases exceeding 16 million metric tons (MMT) in catch, $53 billion in profit, and 619 MMT in biomass relative to business as usual. We also find that, with appropriate reforms, recovery can happen quickly, with the median fishery taking under 10 y to reach recovery targets. Our results show that commonsense reforms to fishery management would dramatically improve overall fish abundance while increasing food security and profits. PMID:27035953

  2. Crossing into the substellar regime in Praesepe

    CERN Document Server

    Chappelle, R J; Steele, I A; Dobbie, P D; Magazzù, A

    2005-01-01

    We present the results of a deep optical 2.6 square degree survey with near infrared follow-up measurements of the intermediate-aged Praesepe open cluster. The survey is complete to I=21.3, Z=20.5, corresponding to ~0.06 Solar Masses assuming a cluster age of 0.5 Gyrs. Using 3-5 pass-bands to constrain cluster membership, we identify 32 new low mass cluster members, at least 4 of which are likely to be substellar. We use the low mass census to trace the region where the sequence moves away from the NEXTGEN towards the Dusty regime at Teff = 2200K. In doing so, we identify four unresolved binaries, yielding a substellar binary fraction of ~30 percent. The binary fractions appear to decrease below 0.1 Solar Masses, in contrast to the rising fractions found in the Pleiades. We also identify a paucity of late M dwarfs, thought to be due to a steepening in the mass-luminosity relation at these spectral types, and compare the properties of this gap in the sequence to those observed in younger clusters. We note an o...

  3. On the ontological emergence from quantum regime

    Energy Technology Data Exchange (ETDEWEB)

    Luty, Damian [Adam Mickiewicz University, Poznan (Poland)

    2014-07-01

    There are several views on the relation between quantum physics and theory of relativity (especially General Relativity, GR). A popular perspective is this: GR with its macroscopic gravitational effects will turn out to be a limit of a more fundamental theory which should consider discrete physics and not deal with continuity (like theory of relativity). Thus, GR will emerge from a more basic theory, which should be quantum-like. One could call this an epistemic emergence view towards fundamental theories. The question is, given that scientific realism is valid: should emergence be a fundamental notion in our ontological view about the evolving, physical Universe? Is there an ontological emergence fully compatible with the notion of fundamentality? I argue that if we want to defend ontological emergence (from quantum to macroscopic regime) as something fundamental, we will arrive at the position of metaphysics of dispositions (and I argue, why this is undesirable), or conclude, that we cannot square fully fundamental ontology with the notion of emergence, and that we have to accept an ontological pluralism relativised to a certain scale. I defend the latter proposition, showing, that epistemic emergence doesn't entail (logically) ontological emergence.

  4. Regime shifts in a social-ecological system

    OpenAIRE

    Lade, Steven J.; Tavoni, Alessandro; Levin, Simon A.; Schlüter, Maja

    2013-01-01

    Ecological regime shifts are rarely purely ecological. Not only is the regime shift frequently triggered by human activity, but the responses of relevant actors to ecological dynamics are often crucial to the development and even existence of the regime shift. Here, we show that the dynamics of human behaviour in response to ecological changes can be crucial in determining the overall dynamics of the system. We find a social-ecological regime shift in a model of harvesters of a common-pool re...

  5. Institutional design and regime effectiveness in transboundary river management – the Elbe water quality regime

    Directory of Open Access Journals (Sweden)

    I. Dombrowsky

    2008-02-01

    Full Text Available The literature on transboundary river management suggests that institutions play an important role in bringing about cooperation. However, knowledge about how such institutions should be designed in order to do so remains limited. One way to learn more about adequate institutional design is to assess the effectiveness of existing regimes, and to trace the causal relationships that lead to the respective outcomes. In order to gain further insights into the relationship between institutional design and regime effectiveness, this paper presents a study on the water quality regime of the International Commission for the Protection of the Elbe (ICPE. The analysis is based on a review of pertinent documents and ten qualitative interviews with Czech and German Commission members and NGO representatives. Particular emphasis has been put on determining the ICPE's specific contribution and the no-regime counterfactual as well as on the perceived expediency of the institutional arrangements. The study shows overall that the countries were relatively successful in improving water quality in the Elbe basin. However, this outcome can only partly be attributed to the ICPE itself. Furthermore, the ICPE's contribution towards achieving the various goals varied significantly between the different areas of activity: it was relatively significant where the main responsibility for action lay with the public authorities, such as in the area of wastewater treatment and the establishment of an international alarm plan and model, but was practically non-existent in the reduction of non-point pollution from agriculture, where success depended on the behavior of individual private actors (farmers. The commission contributed towards problem solving by serving as a forum for the joint identification of priorities for action from a basin-wide perspective. The resulting international obligations increased the power of national water administrations and their access to funds

  6. Varieties of Economic Growth Regimes, Types of Macroeconomic Policies, and Policy Regime: A Post-Keynesian Analysis

    OpenAIRE

    Hiroshi Nishi

    2012-01-01

    This study explores the effects of macroeconomic policies on measures of macroeconomic performance such as growth and inflation by setting up a dynamic post-Keynesian model with government and central bank interventions. In doing so, this study reconsiders the arguments in favor of a policy regime. The model in this paper generates several varieties of economic growth regimes and inflation dynamics. The economic growth regimes are defined by the relationship between economic growth, the incom...

  7. CSDP: Seismology of continental thermal regime

    Energy Technology Data Exchange (ETDEWEB)

    Aki, K.

    1989-04-01

    This is a progress report for the past one year of research (year 2 of 5-year project) under the project titled CSDP: Seismology of Continental Thermal Regime'', in which we proposed to develop seismological interpretation theory and methods applicable to complex structures encountered in continental geothermal areas and apply them to several candidate sites for the Continental Scientific Drilling Project. During the past year, two Ph.D. thesis works were completed under the present project. One is a USC thesis on seismic wave propagation in anisotropic media with application to defining fractures in the earth. The other is a MIT thesis on seismic Q and velocity structure for the magma-hydrothermal system of the Valles Caldera, New Mexico. The P.I. co-organized the first International Workshop on Volcanic Seismology at Capri, Italy in October 1988, and presented the keynote paper on the state-of-art of volcanic seismology''. We presented another paper at the workshop on Assorted Seismic Signals from Kilauea Volcano, Hawaii. Another international meeting, namely, the Chapman Conference on seismic anisotropy in the earth's crust at Berkeley, California in May 1988, was co-organized by the co-P.I. (P.C.L), and we presented our work on seismic waves in heterogeneous and anisotropic media. Adding the publications and presentations made in the past year to the list for the preceding year, the following table lists 21 papers published, submitted or presented in the past two years of the present project. 65 refs., 334 figs., 1 tab.

  8. Dissipation regimes for short wind waves

    Science.gov (United States)

    Caulliez, Guillemette

    2013-02-01

    The dissipation processes affecting short wind waves of centimeter and decimeter scales are investigated experimentally in laboratory. The processes include damping due to molecular viscosity, generation of capillary waves, microbreaking, and breaking. The observations were made in a large wind wave tank for a wide range of fetches and winds, using a laser sheet and a high-resolution video camera. The work aims at constructing a comprehensive picture of dissipative processes in the short wind wave field, to find for which scales particular dissipative mechanism may become important. Four distinct regimes have been identified. For capillary-gravity wave fields, i.e., for dominant waves with scales below 4 cm, viscous damping is found to be the main dissipation mechanism. The gravity-capillary wave fields with dominant wavelength less than 10 cm usually exhibit a train of capillary ripples at the crest wavefront, but no wave breaking. For such waves, the main dissipation process is molecular viscosity occurring through nonlinear energy cascade toward high-frequency motions. Microscale breaking takes place for waves longer than 10 cm and manifests itself in a very localized surface disruption on the forward face of the crest. Such events generate turbulent motions in water and thus enhance wave dissipation. Plunging breaking, characterized by formation of a crest bulge, a microjet hitting the water surface and a splash-up, occurs for short gravity waves of wavelength exceeding 20 cm. Macroscale spilling breaking is also observed for longer waves at high winds. In both cases, the direct momentum transfer from breaking waves to the water flow contributes significantly to wave damping.

  9. The seismology of geothermal regimes. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Aki, K.

    1997-04-01

    The authors have been developing seismological interpretation theory and methods applicable to complex structures encountered in geothermal areas for a better understanding of the earth`s geothermal regimes. The questions the y have addressed in their research may be summarized as ``What is going on in the earth`s crust under tectonically active regions; what are the structures and processes responsible for such activities as earthquakes and volcanic eruptions; and how can one capture their essence effectively by means of seismological studies?`` First, the authors found clear evidence for localization of scattered seismic energy in the deep magmatic system of the volcano on the island of Reunion in the Indian Ocean. The seismic coda of local earthquakes show concentrated energy in the intrusive zones as late as 30 to 40 seconds after the origin time. This offers a very effective method for defining a zone of strong heterogeneity on a regional scale, complementary to the high resolution study using trapped modes as pursued in the past project. Secondly, the authors identified about 700 long-period events with various frequencies and durations from the data collected during the past 5 years which included three episodes of eruption. They are applying a finite-element method to the simplest event with the longest period and the shortest duration in order to find the location, geometry and physical properties of their source deterministically. The preliminary result described here suggests that their sources may be a horizontally lying magma-filled crack at a shallow depth under the summit area. In addition to the above work on the Reunion data, they have continued the theoretical and observational studies of attenuation and scattering of seismic waves.

  10. Antecedents of Teachers Fostering Effort within two different management regimes: An assessment-based accountability regime and regime without external pressure on results

    OpenAIRE

    Knut-Andreas Christophersen; Eyvind Elstad; Are Turmo

    2012-01-01

    This article focuses on the comparison of organizational antecedents of teachers' fostering of students' effort in two quite different accountability regimes: one management regime with an external-accountability system and one with no external accountability devices. The methodology involves cross-sectional surveys from two different management systems: (1) teachers working under assessment-based accountability (N = 236) and (2) folk–high school teachers who work without tests and examinatio...

  11. 血清尿酸水平变化在急性脑梗死患者尿激酶溶栓治疗中的临床意义%Clinical significance of serum uric acid level changes in patients with acute cerebral infarction treated with urokinase

    Institute of Scientific and Technical Information of China (English)

    杨文军; 王丽杰; 陈婉秋; 石小晶; 王帅; 曹信杰; 段宏军; 张春荣

    2012-01-01

    Objective To explore the changes of serum uric acid level before and after the treatment with urokinase in patients with acute cerebral infarction and their clinical significance. Methods Fifty-nine patients with acute cerebral infarction were selected and treated with urokinase. Thirtysix patients were in effective therapy group and 23 patients were in ineffective therapy group according to the result of urokinase therapy. The blood samples of all patients were collected before, on days 2,7 of treatment and at the third month after the onset of acute cerebral infarction, and the serum uric acid levels were detected. Results There were statistically significant differences in the serum uric acid levels between effective therapy and ineffective therapy groups on the second day after urokinase therapy(P0. 05). Conclusion The result suggests that uric acid may play a role during reperfusion in ischemic brain,and the serum uric acid was decreased after urokinase therapy.%目的 探讨急性脑梗死患者溶栓治疗前后血清尿酸水平变化及意义.方法 选择急性脑梗死患者59例,均进行尿激酶治疗,根据治疗效果分为好转组36例和无好转组23例.检测2组溶栓前、溶栓第2、7天及发病3个月后血清尿酸水平.结果 与无好转组比较,好转组溶栓第2天尿酸水平明显下降,差异有统计学意义(P<0.05),2组溶栓治疗前及溶栓第7天以及3个月后尿酸水平比较,差异无统计学意义(P>0.05).结论 尿酸有可能在缺血再灌注过程中发挥一定作用,存在溶栓后尿酸水平衰减.

  12. Antecedents of Teachers Fostering Effort within two different management regimes: An assessment-based accountability regime and regime without external pressure on results

    Directory of Open Access Journals (Sweden)

    Knut-Andreas Christophersen

    2012-12-01

    Full Text Available This article focuses on the comparison of organizational antecedents of teachers' fostering of students' effort in two quite different accountability regimes: one management regime with an external-accountability system and one with no external accountability devices. The methodology involves cross-sectional surveys from two different management systems: (1 teachers working under assessment-based accountability (N = 236 and (2 folk–high school teachers who work without tests and examinations and, thereby, without external accountability devices (N = 366. The purpose of the study is to estimate the path coefficients in structural equation modeling in the two regimes and compare the significance of relationships between concepts in the structural models. Through this comparison, inferences are drawn suggesting how accountability repercussions and other leadership organizational antecedents may influence teachers' fostering of students' efforts and how qualitative aspects among school professionals may influence the fostering of effort. Implications for practice and directions for future research are discussed.Christopherson, K, Elstad, E, & Turmo, A. (2012. Antecedents of teachers fostering effort within two different management regimes: an assessment-based accountability regime and regime without external pressure on results. International Journal of Education Policy and Leadership 7(6. Retrieved from www.ijepl.org.

  13. Transition to ballistic regime for heat transport in helium II

    Energy Technology Data Exchange (ETDEWEB)

    Sciacca, Michele, E-mail: michele.sciacca@unipa.it [Dipartimento Scienze Agrarie e Forestali, Università degli studi di Palermo, Viale delle Scienze, 90128 Palermo (Italy); Departament de Física, Universitat Autònoma de Barcelona, 08193 Bellaterra, Catalonia (Spain); Sellitto, Antonio, E-mail: ant.sellitto@gmail.com [Dipartimento di Matematica, Informatica ed Economia, Università della Basilicata, Campus Macchia Romana, 85100 Potenza (Italy); Jou, David, E-mail: david.jou@uab.cat [Departament de Física, Universitat Autònoma de Barcelona, 08193 Bellaterra, Catalonia (Spain); Institut d' Estudis Catalans, Carme 47, 08001 Barcelona, Catalonia (Spain)

    2014-07-04

    The size-dependent and flux-dependent effective thermal conductivity of narrow capillaries filled with superfluid helium is analyzed from a thermodynamic continuum perspective. The classical Landau evaluation of the effective thermal conductivity of quiescent superfluid, or the Gorter–Mellinck regime of turbulent superfluids, is extended to describe the transition to ballistic regime in narrow channels wherein the radius R is comparable to (or smaller than) the phonon mean-free path ℓ in superfluid helium. To do so, we start from an extended equation for the heat flux incorporating non-local terms, and take into consideration a heat slip flow along the walls of the tube. This leads from an effective thermal conductivity proportional to R{sup 2} (Landau regime) to another one proportional to Rℓ (ballistic regime). We consider two kinds of flows: along cylindrical pipes and along two infinite parallel plates. - Highlights: • Heat transport in counterflow helium in the ballistic regime. • The one-fluid model based on the Extended Thermodynamics is used. • The transition from the Landau regime to the ballistic regime. • The transition from quantum turbulence to ballistic regime.

  14. Consumption, investment and life insurance under different tax regimes

    DEFF Research Database (Denmark)

    Bruun, Kenneth

    2013-01-01

    , and compare tax effects on consumption/savings contributions, investment and purchase of life insurance under the regimes. The optimization problems have analytical solutions, which allow for easy comparison of tax effects under the different regimes. To substantiate the results we also present a numerical...

  15. Bargaining among Nations: Culture, History, and Perceptions in Regime Formation.

    Science.gov (United States)

    Lipschutz, Ronnie D.

    1991-01-01

    The formation of regimes (collective international schemes) for managing global problems depends on culture, history, and perceptions. The ways in which these elements affect bargaining among nations over issues of the global commons are discussed. Implications are reviewed for a regime to deal with atmospheric conditions and global warming. (SLD)

  16. Disciplinary Regimes of "Care" and Complementary Alternative Education

    Science.gov (United States)

    Thomson, Pat; Pennacchia, Jodie

    2016-01-01

    In schools, the notion of "care" is often synonymous with welfare and disciplinary regimes. Drawing on Foucault, and a study of alternative education (AE) across the UK, and looking in depth at two cases of complementary AE, we identify three types of disciplinary regimes at work in schools: (1) dominant performative reward and…

  17. Transition to ballistic regime for heat transport in helium II

    International Nuclear Information System (INIS)

    The size-dependent and flux-dependent effective thermal conductivity of narrow capillaries filled with superfluid helium is analyzed from a thermodynamic continuum perspective. The classical Landau evaluation of the effective thermal conductivity of quiescent superfluid, or the Gorter–Mellinck regime of turbulent superfluids, is extended to describe the transition to ballistic regime in narrow channels wherein the radius R is comparable to (or smaller than) the phonon mean-free path ℓ in superfluid helium. To do so, we start from an extended equation for the heat flux incorporating non-local terms, and take into consideration a heat slip flow along the walls of the tube. This leads from an effective thermal conductivity proportional to R2 (Landau regime) to another one proportional to Rℓ (ballistic regime). We consider two kinds of flows: along cylindrical pipes and along two infinite parallel plates. - Highlights: • Heat transport in counterflow helium in the ballistic regime. • The one-fluid model based on the Extended Thermodynamics is used. • The transition from the Landau regime to the ballistic regime. • The transition from quantum turbulence to ballistic regime

  18. States agree on stronger physical protection regime

    International Nuclear Information System (INIS)

    Full text: Delegates from 89 countries agreed on 8 July to fundamental changes that will substantially strengthen the Convention on the Physical Protection of Nuclear Material (CPPNM). IAEA Director General Mohamed ElBaradei welcomed the agreement in saying 'This new and stronger treaty is an important step towards greater nuclear security by combating, preventing, and ultimately punishing those who would engage in nuclear theft, sabotage or even terrorism. It demonstrates that there is indeed a global commitment to remedy weaknesses in our nuclear security regime.' The amended CPPNM makes it legally binding for States Parties to protect nuclear facilities and material in peaceful domestic use, storage as well as transport. It will also provide for expanded cooperation between and among States regarding rapid measures to locate and recover stolen or smuggled nuclear material, mitigate any radiological consequences of sabotage, and prevent and combat related offences. The original CPPNM applied only to nuclear material in international transport. Conference President Dr. Alec Baer said 'All 89 delegations demonstrated real unity of purpose. They put aside some very genuine national concerns in favour of the global interest and the result is a much improved convention that is better suited to addressing the nuclear security challenges we currently face.' The new rules will come into effect once they have been ratified by two-thirds of the 112 States Parties of the Convention, expected to take several years. 'But concrete actions are already taking place around the world. For more than 3 years, the IAEA has been implementing a systematic Nuclear Security plan, including physical protection activities designed to prevent, detect and respond to malicious acts,' said Anita Nillson, Director of the IAEA's Office of Nuclear Security. The Agency's Nuclear Security Fund, set up after the events of 9/11, has delivered $19.5 million in practical assistance to 121 countries

  19. Boundary between Stable and Unstable Regimes of Accretion. Ordered and Chaotic Unstable Regimes

    CERN Document Server

    Blinova, A A; Lovelace, R V E

    2015-01-01

    We search for the boundary between stable and Rayleigh-Taylor unstable regimes of accretion to magnetized stars in a new set of high grid resolution simulations. We found that the boundary between stable and unstable regimes is mainly determined by the ratio of the corotation radius r_cor (where the Keplerian angular velocity in the disc matches the angular velocity of the star) to the magnetospheric radius r_m (where the magnetic stress in the magnetosphere matches the matter stress in the disc). Instability is stronger when r_cor is larger with respect to r_m, that is, when the gravitational force is larger than the centrifugal force at the inner disc. In the cases of a small tilt of the magnetosphere, Theta=5 deg, and a small alpha-parameter of viscosity, alpha=0.02, the boundary is located at r_cor approx. 1.4 r_m. Instability becomes stronger at higher values of viscosity, and occurs at lower values of r_cor/r_m. At higher values of Theta, the variability associated with instability decreases. Simulation...

  20. Study on Effect of Shenmai Injection Protecting Myocardium against Ischemia-Reperfusion Injury in Thrombolytic Therapy with Urokinase for Acute Myocardial Infarction Patient Evaluated by 99mTc-MIBI Myocardial Imaging

    Institute of Scientific and Technical Information of China (English)

    郭松鹏; 张言镇

    2001-01-01

    Objective: To evaluate the myocardial protecting effect of Shenmai injection (SMI) against ischemia/reperfusion injury in thrombolytic therapy with urokinase (UK) for acute myocardial infarction patients by 99mTc-MIBI myocardial imaging (SPECT). Methods: Five hundred and thirty-seven patients were divided into two groups randomly. The SMI group (n=292) was treated with thrombolytictreatment plus SMI and the control group (n=245) with thrombolytic treatment solely. Single photon emission computed tomography (SPECT) was carried out on the 7th day after thrombolysis to determine the ischemic myocardial area (IMA) and ejection fraction (EF) in both groups and compared. Results: The infarction related area (IRA) of reperfusion rate in the two groups was not different significantly (72.26% vs 72.65%, P >0.05). The IMA in patients of the SMI group, no matter with or without reperfused IRA (211 cases and 81 cases) respectively, was significantly lower than that in the control group (178 cases and 67 cases) respectively, P<0.01 and P<0.05 respectively. The EF value in the SMI group was significantly higher than that in the control group (P<0.01). Conclusion:Using SMI in early stage of thrombolytic treatment in acute myocardial infarction could significantly reduce IMA and increase EF. SMI showed good protective effect against myocardial ischemia/reperfusion injury in thrombolytic treatment.

  1. Study on Effect of Shenmai Injection Protecting Myocardium against Ischemia-Reperfusion Injury in Thrombolytic Therapy with Urokinase for Acute Myocardial Infarction Patient Evaluated by 99mTc-MIBI Myocardial Imaging

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective: To evaluate the myocardial protecting effect of Shenmai injection (SMI) against ischemia/reperfusion injury in thrombolytic therapy with urokinase (UK) for acute myocardial infarction patients by 99mTc-MIBI myocardial imaging (SPECT). Methods: Five hundred and thirty-seven patients were divided into two groups randomly. The SMI group (n=292) was treated with thrombolytictreatment plus SMI and the control group (n=245) with thrombolytic treatment solely. Single photon emission computed tomography (SPECT) was carried out on the 7th day after thrombolysis to determine the ischemic myocardial area (IMA) and ejection fraction (EF) in both groups and compared. Results: The infarction related area (IRA) of reperfusion rate in the two groups was not different significantly (72.26% vs 72.65%, P >0.05). The IMA in patients of the SMI group, no matter with or without reperfused IRA (211 cases and 81 cases) respectively, was significantly lower than that in the control group (178 cases and 67 cases) respectively, P<0.01 and P<0.05 respectively. The EF value in the SMI group was significantly higher than that in the control group (P<0.01). Conclusion:Using SMI in early stage of thrombolytic treatment in acute myocardial infarction could significantly reduce IMA and increase EF. SMI showed good protective effect against myocardial ischemia/reperfusion injury in thrombolytic treatment.

  2. An Examination of the Nature of Global MODIS Cloud Regimes

    Science.gov (United States)

    Oreopoulos, Lazaros; Cho, Nayeong; Lee, Dongmin; Kato, Seiji; Huffman, George J.

    2014-01-01

    We introduce global cloud regimes (previously also referred to as "weather states") derived from cloud retrievals that use measurements by the Moderate Resolution Imaging Spectroradiometer (MODIS) instrument aboard the Aqua and Terra satellites. The regimes are obtained by applying clustering analysis on joint histograms of retrieved cloud top pressure and cloud optical thickness. By employing a compositing approach on data sets from satellites and other sources, we examine regime structural and thermodynamical characteristics. We establish that the MODIS cloud regimes tend to form in distinct dynamical and thermodynamical environments and have diverse profiles of cloud fraction and water content. When compositing radiative fluxes from the Clouds and the Earth's Radiant Energy System instrument and surface precipitation from the Global Precipitation Climatology Project, we find that regimes with a radiative warming effect on the atmosphere also produce the largest implied latent heat. Taken as a whole, the results of the study corroborate the usefulness of the cloud regime concept, reaffirm the fundamental nature of the regimes as appropriate building blocks for cloud system classification, clarify their association with standard cloud types, and underscore their distinct radiative and hydrological signatures.

  3. Marine regime shifts: drivers and impacts on ecosystems services

    Science.gov (United States)

    Rocha, J.; Yletyinen, J.; Biggs, R.; Blenckner, T.; Peterson, G.

    2015-01-01

    Marine ecosystems can experience regime shifts, in which they shift from being organized around one set of mutually reinforcing structures and processes to another. Anthropogenic global change has broadly increased a wide variety of processes that can drive regime shifts. To assess the vulnerability of marine ecosystems to such shifts and their potential consequences, we reviewed the scientific literature for 13 types of marine regime shifts and used networks to conduct an analysis of co-occurrence of drivers and ecosystem service impacts. We found that regime shifts are caused by multiple drivers and have multiple consequences that co-occur in a non-random pattern. Drivers related to food production, climate change and coastal development are the most common co-occurring causes of regime shifts, while cultural services, biodiversity and primary production are the most common cluster of ecosystem services affected. These clusters prioritize sets of drivers for management and highlight the need for coordinated actions across multiple drivers and scales to reduce the risk of marine regime shifts. Managerial strategies are likely to fail if they only address well-understood or data-rich variables, and international cooperation and polycentric institutions will be critical to implement and coordinate action across the scales at which different drivers operate. By better understanding these underlying patterns, we hope to inform the development of managerial strategies to reduce the risk of high-impact marine regime shifts, especially for areas of the world where data are not available or monitoring programmes are not in place.

  4. Shearing box simulations in the Rayleigh unstable regime

    CERN Document Server

    Nauman, Farrukh

    2015-01-01

    We study the stability properties of Rayleigh unstable flows both in the purely hydrodynamic and magnetohydrodynamic (MHD) regimes for two different values of the shear $q=2.1, 4.2$ ($q = - d\\ln\\Omega / d\\ln r$) and compare it with the Keplerian case $q=1.5$. The Rayleigh stability criterion states that hydrodynamic shear flows are stable for $q2$ regime as the volume averaged velocities ($k=0$ mode) are unstable in this regime but the advantage of using a pseudospectral code is that the $k=0$ mode is conserved. We find that the $q>2$ regime is unstable to turbulence both in the hydrodynamic and in the MHD limit (with an initially weak magnetic field). In the $q>2$ regime, the velocity fluctuations dominate the magnetic fluctuations whereas in the $q2$ regime the instability produces primarily velocity fluctuations that cause magnetic fluctuations, with the causality reversed for the $q<2$ MRI unstable regime. We also find that the magnetic field correlation is increasingly localized as the shear is increa...

  5. Perspectives on the International and Internal Nuclear Security Regime

    Energy Technology Data Exchange (ETDEWEB)

    Jang, Sung Soon [Korea Nuclear Non-proliferation and Control, Daejeon (Korea, Republic of)

    2015-10-15

    The term, 'Nuclear Security' became more familiar to Korean public after the government hosted 2012 Seoul Nuclear Security Summit. Nuclear Security is prevention of, detection of and response to criminal or intentional unauthorized acts involving or directed at nuclear material, other radioactive material, associated facilities, or associated activities. Nuclear Security includes physical protection, security of radioactive sources, nuclear forensics, nuclear smuggling prevention, border monitoring, and cyber security with regard to nuclear and other radiological materials. This abstract will review recent international trends and discuss the nuclear security regime in the Republic of Korea (ROK). The international Nuclear Security Regime has been strengthened. The upcoming Chicago Summit in 2016 and the entry into force of the Amendment of Convention on Physical Protection of Nuclear Materials (CPPNM) will bring major changes in nuclear security. The Republic of Korea hosted the 2012 Seoul Summit and strengthened domestic regime to meet international standards. The ROK has worked hard to contribute to the international security regime and to establish a robust domestic security regime against terrorist threats. Even if the nuclear security regime is robust, Risk-informed Nuclear Security management should be established to meet international standards and to implement effective as well as an efficient nuclear security regime.

  6. Conceptualization of UN Peacekeeping as an International Regime

    Directory of Open Access Journals (Sweden)

    Jana Urbanovská

    2012-12-01

    Full Text Available It is widely recognized that UN peacekeeping operations are an undertheorized topic. In this respect, scholars call to devote more attention to the incorporation of the study of UN peacekeeping operations into the framework of international relations theories. The article responds to these calls and suggests conceptualizing UN peacekeeping as an international regime. Some authors have already referred to UN peacekeeping as an international regime, however, the references have been rather fragmentary and there is no detailed identification of individual dimensions of this regime. The article uses the conception of Stephen Krasner who defines regimes as “principles, norms, rules, and decision-making procedures around which actors’ expectations converge in a given area of international relations” to carefully identify and characterize the dimensions of the UN peacekeeping regime. The conceptualization of UN peacekeeping as an international regime is understood as a first and necessary step to opening new ground to the theoretical study of UN peacekeeping operations within the regime analysis.

  7. The NPT regime: Progress and promises

    International Nuclear Information System (INIS)

    Full text: Thesis. The 'NPT regime' has arrived at a fateful crossroads. Though extended indefinitely in 1995, its future is my no means secure. The future 'progress' of this treaty will depend upon whether the 'promises' of its States parties are fully implemented and, eventually, upon the treaty's success in achieving fully universal membership. Challenges The treaty faces many short-term and longer-term challenges: Short term - The first Preparatory Committee meeting for the 2005 Review Conference will meet next year. NNWS will want to see some evidence of progress on nuclear disarmament (Art. VI), along the lines prescribed in the 13 'practical steps' agreed at the last Review Conference. Yet progress has been set back by: uncertainties over the future of the ABM Treaty; the failure of START II and the CTBT to enter into force; the lack of a FISMAT treaty and a treaty establishing a NWFZ in Central Asia; continued qualitative improvements in nuclear weapons; hints that nuclear testing may one day resume; the persistence of doctrines of first-use, pre-emptive use, and use against states that use CBW. Other compliance-related questions will arise over safeguards (e.g. the inability of the IAEA to conduct inspections in the DPRK; signs of a breakdown of the norm of full-scope IAEA safeguards, e.g. in South Asia). There are also concerns over the implementation of non-proliferation commitments (e.g. persisting allegations about nuclear weapon programmes in existing NNWS). The terrorist attacks in New York and Washington, D.C. on 11 September should also serve as a reminder of the new terrorist dangers relating to the possible use of weapons of mass destruction and unorthodox delivery systems. Longer term - Selectivity in the enforcement of NPT norms; unilateralism; IAEA funding uncertainties and shortfalls; difficulties in reaching universal membership (India, Pakistan, and Israel); continuing compliance problems with respect to both non-proliferation and

  8. Past and Future Drought Regimes in Turkey

    Science.gov (United States)

    Sen, Burak; Topcu, Sevilay; Turkes, Murat; Sen, Baha

    2010-05-01

    Climate variability in the 20th century was characterized by apparent precipitation variability at both temporal and spatial scales. In addition to the well-known characteristic seasonal and year-to-year variability, some marked and long-term changes in precipitation occurred in Turkey, particularly after the early 1970s. Drought, originating from a deficiency of precipitation over an extended time period (which is usually a season or more) has become a recurring phenomenon in Turkey in the past few decades. Spatially coherent with the significant drought events since early 1970s, water stress and shortages for all water user sectors have also reached their critical points in Turkey. Analyzing the historical occurrence of drought provides an understanding of the range of climate possibilities for a country, resulting in more informed management decision-making. However, future projections about spatial and temporal changes in drought characteristics such as frequency, intensity and duration can be challenging for developing appropriate mitigation and adaptation strategies. Hence, the objectives of this study are (i) to analyze the spatial and temporal dimensions of historical droughts in Turkey, (2) to predict potential intensity, frequency and duration of droughts in Turkey for the future (2070-2100). The Standardized Precipitation Index (SPI) and the Percent to Normal Index (PNI) have been used to assess the drought characteristics. Rainfall datasets for the reference period, 1960-1990, were acquired from 52 stations (representative of all kinds of regions with different rainfall regimes in the country) of the Turkish State Meteorological Service (TSMS). The future rainfall series for the 2070-2100 period were simulated using a regional climate model (RegCM3) for IPCC's SRESS-A2 scenario conditions. For verification of RegCM3 simulations, the model was performed for the reference period and simulated rainfall data were used for computing two drought indices (SPI

  9. Considerations on nonproliferation regime meeting in a changing world

    Energy Technology Data Exchange (ETDEWEB)

    Kurihara, Hiroyoshi; Kikuchi, Masahiro [Nuclear Material Control Center, Tokyo (Japan)

    1994-12-31

    This paper summarizes the past history of worldwide nonproliferation regime, then proposes the future improvements on the regime. Present worldwide nonproliferation regime have been formulated during the cold war era. Therefore, the structure and measures of the regime were heavily influenced by the features of cold war era. Though the cold war was over, still new international order does not seem to be on the horizon, we need to review the present regime and to improve the regime compatible to new world situation. Generally speaking, the nonproliferation regime have gained moderate success so far. We could point out the following features as a kind of success: (1) No increase of overt Nuclear Weapon State (NWS), (2) All five NWSs have finally participated to the NPT, (3) South Africa has destroyed its nuclear weapons and became Non-Nuclear Weapon State (NNWS), (4) Successful conclusions of some regional arrangements, such as Tlatelolco, Ralotonga, and (5) Strengthening of export control on sensitive items. On the other hand, we recognize the following points as the failures of the regime. (6) India, Pakistan and Israel reject to join the NPT, (7) Existence of some violation against NPT regime, i.e. Iraqi case and DPRK case, (8) Insufficient effective measures against brain drain problem, (9) Risk exists for the long term extension of NPT, and (10) Insufficient flexibility to meet changing boundary conditions. We would propose the various measures for strengthening to meet changing boundary conditions, as follows: (11) Measures to be taken along with future civil use of Plutonium, (12) Strengthening and rationalizing international safeguards, (13) Countermeasures for emerging new types of nuclear proliferation, (14) Strengthening nuclear material control in NWS, (15) Measures to be taken for nuclear material from dismantled nuclear weapons, and (16) Nuclear disarmament. (author).

  10. Laserlike and atomlike regimes in a one-atom laser

    OpenAIRE

    Boozer, A. D.

    2008-01-01

    We consider a three-level model of a one-atom laser, and show that there are two limiting regimes of parameter space, one in which the system behaves like a conventional laser and one in which the system exhibits novel quantum properties. We show that in the first limiting regime, the model can be approximated by semiclassical laser theory, and in the second limiting regime the model can be approximated as an effective two-level atom. We also perform numerical simulations that confirm the lim...

  11. Diversity-Multiplexing Tradeoff in the Low-SNR Regime

    CERN Document Server

    Loyka, Sergey

    2011-01-01

    An extension of the popular diversity-multiplexing tradeoff framework to the low-SNR (or wideband) regime is proposed. The concept of diversity gain is shown to be redundant in this regime since the outage probability is SNR-independent and depends on the multiplexing gain and the channel power gain statistics only. The outage probability under the DMT framework is obtained in an explicit, closed form for a broad class of channels. The low and high-SNR regime boundaries are explicitly determined for the scalar Rayleigh-fading channel, indicating a significant limitation of the SNR-asymptotic DMT when the multiplexing gain is small.

  12. Nonlinear Regime of a Single-mode CSR Instability

    International Nuclear Information System (INIS)

    Close to the shielding threshold of synchrotron radiation in a toroidal waveguide the coherent synchrotron radiation (CSR) instability may be driven by a single synchronous mode. The linear analysis of the problem is presented elsewhere. In this paper we study a nonlinear regime of the single-mode CSR instability. We show that in the nonlinear regime the instability is described by a system of equations which, after a proper scaling, coincides with the 1D FEL instability theory. We present numeric and analytic analysis of the non-linear regime for the time interval large compared with the damping time taking into account radiation damping and diffusion due to the quantum fluctuations

  13. Formations and transformations of language regimes: Turkey, a case study

    OpenAIRE

    Balçık, Mehmet Berk; Balcik, Mehmet Berk

    2009-01-01

    There are two main aims of this dissertation: to present a legal and ideological history of the formation of the language regime in Turkey in the Republican period; and to analyze its transformation in the post-1980 era. A language regime is defined in this dissertation as a de jure or de facto regulation of the linguistic behavior, in its content or in its status, within a space of communicative action, such as that of a nation-state or a speech group. In other words, a language regime is a ...

  14. Raman amplification in the broken-wave regime

    CERN Document Server

    Farmer, John P

    2015-01-01

    In regimes far beyond the wavebreaking theshold of Raman amplification, we show that significant amplifcation can occur after the onset of wavebreaking, before phase mixing destroys the coupling between pump and probe. The amplification efficiency in this regime is therefore strongly dependent on the energy-transfer rate when wavebreaking occurs, and is, as such, sensitive to both the probe amplitude and profile. In order to access the higher-efficiency broken-wave regime, a short, intense probe is required. Parameter scans show the marked difference in behaviour compared to below wavebreaking, where longer, more energetic pulses lead to improved efficiencies.

  15. Policy Ideas, Knowledge Regimes and Comparative Political Economy

    DEFF Research Database (Denmark)

    Campbell, John L.; Pedersen, Ove K.

    2015-01-01

    Despite much attention to how ideas affect policy making, where these ideas come from is a blind spot in comparative political economy. We show that an important source of policy ideas are knowledge regimes—fields of policy research organizations. We show as well that the organization of knowledge...... regimes is heavily influenced by the organization of their surrounding political economies such that knowledge regimes have particular national characters. Furthermore, when people perceive that the utility of their knowledge regime for the rest of the political economy breaks down, they often try......, France and Germany. Theoretical implications are explored for two literatures in comparative political economy: ideational analysis and the varieties of capitalism....

  16. Regime-Switching Risk: To Price or Not to Price?

    Directory of Open Access Journals (Sweden)

    Tak Kuen Siu

    2011-01-01

    “normative” issues to be addressed in pricing contingent claims under a Markovian, regime-switching, Black-Scholes-Merton model. We address this issue using a minimal relative entropy approach. Firstly, we apply a martingale representation for a double martingale to characterize the canonical space of equivalent martingale measures which may be viewed as the largest space of equivalent martingale measures to incorporate both the diffusion risk and the regime-switching risk. Then we show that an optimal equivalent martingale measure over the canonical space selected by minimizing the relative entropy between an equivalent martingale measure and the real-world probability measure does not price the regime-switching risk. The optimal measure also justifies the use of the Esscher transform for option valuation in the regime-switching market.

  17. Regime shifts and resilience in China's coastal ecosystems.

    Science.gov (United States)

    Zhang, Ke

    2016-02-01

    Regime shift often results in large, abrupt, and persistent changes in the provision of ecosystem services and can therefore have significant impacts on human wellbeing. Understanding regime shifts has profound implications for ecosystem recovery and management. China's coastal ecosystems have experienced substantial deterioration within the past decades, at a scale and speed the world has never seen before. Yet, information about this coastal ecosystem change from a dynamics perspective is quite limited. In this review, I synthesize existing information on coastal ecosystem regime shifts in China and discuss their interactions and cascading effects. The accumulation of regime shifts in China's coastal ecosystems suggests that the desired system resilience has been profoundly eroded, increasing the potential of abrupt shifts to undesirable states at a larger scale, especially given multiple escalating pressures. Policy and management strategies need to incorporate resilience approaches in order to cope with future challenges and avoid major losses in China's coastal ecosystem services.

  18. Spacetime-noncommutativity regime of Loop Quantum Gravity

    CERN Document Server

    Amelino-Camelia, Giovanni; Ronco, Michele; Cesarini, Lorenzo; Lecian, Orchidea Maria

    2016-01-01

    A recent study by Bojowald and Paily provided a path toward the identification of an effective quantum-spacetime picture of Loop Quantum Gravity, applicable in the "Minkowski regime", the regime where the large-scale (coarse-grained) spacetime metric is flat. A pivotal role in the analysis is played by Loop-Quantum-Gravity-based modifications to the hypersurface deformation algebra, which leave a trace in the Minkowski regime. We here show that the symmetry-algebra results reported by Bojowald and Paily are consistent with a description of spacetime in the Minkowski regime given in terms of the $\\kappa$-Minkowski noncommutative spacetime, whose relevance for the study of the quantum-gravity problem had already been proposed for independent reasons.

  19. A new regime for future telecommunication network operations

    NARCIS (Netherlands)

    Gustafsson, S.; Trommelen, P.H.; Vos, C. de

    2010-01-01

    The increasingly crucial role that telecommunications plays in today's information society calls for a new regime for telecommunication network operations. An unbundling of business roles and associated responsibilities is proposed, providing more economic network operations and higher availability

  20. The Hadley and Rossby regimes in a spherical atmosphere

    Science.gov (United States)

    Feldstein, S. B.; Clark, J. H. E.

    1985-01-01

    The properties of the steady Hadley and Rossby regimes for a thermally forced rotating fluid on a sphere are studied. The two layer modified geostrophic model is employed which allows for thermal advection by the divergent wind and time dependent static stability. Heating processes are parameterized using the Newtonian approximation and Rayleigh friction is accounted for. The equations are transformed to spectral form using spherical harmonics and then truncated retaining a simple axisymmetric state and initial, one wave. A time independent Hadley circulation is obtained which is neutral to axisymmetric disturbances but unstable to wave like perturbations for intermediate values of the meridional temperature gradient, indicating the existence of both an upper and lower symmetric Hadley regime. An analytical solution for the steady Rossby circulation is determined for values of the meridional temperature gradient where the Hadley regime is unstable. Linear perturbation theory is used to show that within the steady Rossby regime two or more waves cannot exist simultaneously.

  1. Capillary Breakup of a Liquid Bridge: Identifying Regimes and Transitions

    CERN Document Server

    Li, Yuan

    2016-01-01

    Computations of the breakup of a liquid bridge are used to establish the limits of applicability of similarity solutions derived for different breakup regimes. These regimes are based on particular viscous-inertial balances, that is different limits of the Ohnesorge number $Oh$. To accurately establish the transitions between regimes, the minimum bridge radius is resolved through four orders of magnitude using a purpose-built multiscale finite element method. This allows us to construct a quantitative phase diagram for the breakup phenomenon which includes the appearance of a recently discovered low-$Oh$ viscous regime. The method used to quantify the accuracy of the similarity solutions allows us to identify a number of previously unobserved features of the breakup, most notably an oscillatory convergence towards the viscous-inertial similarity solution. Finally, we discuss how the new findings open up a number of challenges for both theoretical and experimental analysis.

  2. Determination of three characteristic regimes of weakly charged polyelectrolytes monolayers

    International Nuclear Information System (INIS)

    We have demonstrated that monolayer films of randomly charged polystyrene sulfonated acid (PSSA) can be produced by the Langmuir technique, and observed the micro-domain structures, produced by the phase separation of electrostatically charged moieties and the hydrophobic moieties. Using atomic force microscopy and Langmuir isotherm, we found three specific regimes for the polyelectrolytes with various degrees of sulfonation (4-35%); very low charged PSSA (4-5%) in the hydrophobic regime, moderately charged PSSA (6-16%) which possessed a well-balanced nature between electrostatic and the hydrophobic interactions, and strongly amphiphilic nature of PSSA (6-16%) in the ionomer regime. Finally, we could categorize PSSA 35% in the polyelectrolyte regime, due to the dominance of the electrostatic interactions over the hydrophobic interactions

  3. Evolving International Trade and Monetary Regimes and Related Policy Issues

    OpenAIRE

    Kumiharu Shigehara

    1991-01-01

    This essay focuses on some aspects of policy issues relating to evolving international trade and monetary regimes, and patterns of national saving and investment balances conducive to better global economic performance.

  4. Quantifying flooding regime in floodplain forests to guide river restoration

    OpenAIRE

    Christian O. Marks; Keith H Nislow; Magilligan, Francis J.

    2014-01-01

    Abstract Determining the flooding regime needed to support distinctive floodplain forests is essential for effective river conservation under the ubiquitous human alteration of river flows characteristic of the Anthropocene Era. At over 100 sites throughout the Connecticut River basin, the largest river system in New England, we characterized species composition, valley and channel morphology, and hydrologic regime to define conditions promoting distinct floodplain forest assemblages. Species...

  5. Shock-Induced Flows through Packed Beds: Transient Regimes

    CERN Document Server

    Shtemler, Yuri M; Britan, Alex

    2006-01-01

    The early stage of the transient regimes in the shock-induced flows within solid-packed beds are investigated in the linear longwave and high-frequency approximation. The transient resistance law is refined as the Duhameltime integral that follows from the general concept of dynamic tortuosity and compressibility of the packed beds. A closed-form solution is expected to describe accurately the early stage of the transient regime flow and is in qualitative agreement with available experimental data.

  6. Capital Controls and the Timing of Exchange Regime Collapse

    OpenAIRE

    Daekuen Park; Jeffrey Sachs

    1987-01-01

    This paper investigates the nature of balance of payments crises in regimes with capital controls. It extends earlier work on capital controls by assuming that households manage their consumption and asset portfolios to maximize intertemporal utility. Our main result is that capital controls are effective in delaying, but not preventing, a breakdown of a fixed exchange rate regime in the presence of money-financed fiscal deficits.

  7. Constructing a Transnational Timber Legality Assurance Regime: Architecture, Accomplishments, Challenges

    OpenAIRE

    Overdevest, C.; Zeitlin, J

    2013-01-01

    The emerging transnational timber legality assurance regime comprises a set of interrelated policy instruments, both public and private, aimed at controlling trade in illegally logged wood and wood products. The potentially productive interactions among these instruments in the emerging forestry regime create prospects for engendering learning, stimulating cross-fertilization, and enhancing accountability. In this article, we analyze the EU's Forest Law Enforcement Governance and Trade (FLEGT...

  8. Were there regime switches in U.S. monetary policy?

    OpenAIRE

    Christopher A. Sims; Zha, Tao

    2004-01-01

    A multivariate model, identifying monetary policy and allowing for simultaneity and regime switching in coefficients and variances, is confronted with U.S. data since 1959. The best fit is with a model that allows time variation in structural disturbance variances only. Among models that also allow for changes in equation coefficients, the best fit is for a model that allows coefficients to change only in the monetary policy rule. That model allows switching among three main regimes and one r...

  9. Laboratory experiment on boundaries of upper stage plane bed regime

    Science.gov (United States)

    Zrostlík, Štěpán; Matoušek, Václav

    2016-04-01

    Results are discussed of laboratory experiments on criteria determining the transition between the regime of dunes and the upper stage plane bed (UPB) regime and the transition between the UPB regime and the regime of wavy flow. The experiments were carried for 3 fractions of plastic material and two fractions of glass beads in a broad range of flow conditions (different discharges of water and solids and longitudinal bed slopes) in a tilting flume. The experiments reveal that, contrary to expectations, a constant value of the Shields parameter is not an appropriate criterion for the transition between the dune regime and the UPB regime. Furthermore, the transition appears to be insensitive to the total discharge of solids and water. Instead, the criterion seems to be well represented by a constant value of the average transport concentration of sediment (the ratio of volumetric discharge of solids and volumetric discharge of mixture). The experimental results exhibit a very tight correlation between the transport concentration and the longitudinal bed slope. Hence, a constant value of the bed slope can be considered an appropriate criterion for the transition. The transition between the UPB regime and the wavy regime (significant waves develop but they are not always standing waves) is found at a constant value of Froude number, which is in agreement with literature, although it is found at a higher value than the literature usually suggests (Fr = 1.2 instead of 1.0). Hence, the transition occurs in the super-critical flow but it is not necessarily associated with the critical flow.

  10. Generating Schemes for Long Memory Processes: Regimes, Aggregation and Linearity

    OpenAIRE

    Davidson, James; Sibbertsen, Philipp

    2002-01-01

    This paper analyses a class of nonlinear time series models exhibiting long memory. These processes exhibit short memory fluctuations around a local mean (regime) which switches randomly such that the durations of the regimes follow a power law. We show that if a large number of independent copies of such a process are aggregated, the resulting processes are Gaussian, have a linear representation, and converge after normalisation to fractional Brownian motion. Two cases arise, a stationary ca...

  11. Et dansk regime for overgang fra uddannelse til arbejde?

    DEFF Research Database (Denmark)

    Jørgensen, Christian Helms

    2008-01-01

    I dette kapitel vil jeg først gennemgå en række forskningstilgange som på forskellig måde kan begribe de særlige træk ved unges overgang i Danmark i det man kan kalde et dansk 'regime for overgang' (transition regime). Dernæst følger en mere beskrivende gennemgang af det danske uddannelsessystem ...

  12. Real Output and Prices Adjustments under Different Exchange Rate Regimes

    OpenAIRE

    Rajmund MIRDALA

    2013-01-01

    Exchange rate regimes evolution in the European transition economies refers to one of the most crucial policy decision in the beginning of the 1990s employed during the initial stages of the transition process. During the period of last two decades we may identify some crucial milestones in the exchange rate regimes evolution in the European transition economies. due to existing diversity in exchange rate arrangements in the European transition economies in the pre-ERM2 period there seems to ...

  13. Sensitivity of streamflows to hydroclimatic fluctuations: resilience and regime shifts

    Science.gov (United States)

    Botter, Gianluca; Basso, Stefano; Rodriguez-Iturbe, Ignacio; Rinaldo, Andrea

    2016-04-01

    Landscape and climate alterations foreshadow global-scale shifts of river flow regimes. However, a theory that identifies the range of foreseen impacts on streamflows resulting from inhomogeneous forcings and sensitivity gradients across diverse regimes is lacking. In this contribution, we use a dimensionless index embedding simple climate and landscape attributes (the ratio of the mean interarrival of streamflow-producing rainfall events and the mean catchment response time) to discriminate erratic regimes with enhanced intra-seasonal streamflow variability from persistent regimes endowed with regular flow patterns. The proposed classification is successfully applied to 110 seasonal streamflow distributions observed in 44 catchments of the Alps and the United States, allowing the identification of emerging patterns in space and time. In the same framework, the impact of multi-scale fluctuations of the underlying climatic drivers (temperature, precipitation) on the streamflow distributions can be analyzed. Theoretical and empirical data show that erratic regimes, typical of rivers with low mean discharges, are highly resilient in that they hold a reduced sensitivity to variations in the external forcing. Specific temporal trajectories of streamflow distributions and flow regime shifts driven by land-cover change and rainfall patterns can be also evidenced. The approach developed offers an objective basis for the analysis and prediction of the impact of climate/landscape change on water resources.

  14. International regime formation: Ozone depletion and global climate change

    Energy Technology Data Exchange (ETDEWEB)

    Busmann, N.E.

    1994-03-01

    Two theoretical perspectives, neorealism and neoliberal institutionalism, dominate in international relations. An assessment is made of whether these perspectives provide compelling explanations of why a regime with specific targets and timetables was formed for ozone depletion, while a regime with such specificity was not formed for global climate change. In so doing, the assumptions underlying neorealism and neoliberal institutionalism are examined. A preliminary assessment is offered of the policymaking and institutional bargaining process. Patterns of interstate behavior are evolving toward broader forms of cooperation, at least with regard to global environmental issues, although this process is both slow and cautious. State coalitions on specific issues are not yet powerful enough to create a strong community of states in which states are willing to devolve power to international institutions. It is shown that regime analysis is a useful analytic framework, but it should not be mistaken for theory. Regime analysis provides an organizational framework offering a set of questions regarding the principles and norms that govern cooperation and conflict in an issue area, and whether forces independent of states exist which affect the scope of state behavior. An examination of both neorealism and neoliberal institutionalism, embodied by four approaches to regime formation, demonstrates that neither has sufficient scope to account for contextual dynamics in either the ozone depletion or global climate change regime formation processes. 261 refs.

  15. Development of Risk Assessment Methodology for State's Nuclear Security Regime

    International Nuclear Information System (INIS)

    Threats of nuclear terrorism are increasing after 9/11 terrorist attack. Treats include nuclear explosive device (NED) made by terrorist groups, radiological damage caused by a sabotage aiming nuclear facilities, and radiological dispersion device (RDD), which is also called 'dirty bomb'. In 9/11, Al Qaeda planed to cause radiological consequences by the crash of a nuclear power plant and the captured airplane. The evidence of a dirty bomb experiment was found in Afganistan by the UK intelligence agency. Thus, the international communities including the IAEA work substantial efforts. The leaders of 47 nations attended the 2010 nuclear security summit hosted by President Obama, while the next global nuclear summit will be held in Seoul, 2012. Most states established and are maintaining state's nuclear security regime because of the increasing threat and the international obligations. However, each state's nuclear security regime is different and depends on the state's environment. The methodology for the assessment of state's nuclear security regime is necessary to design and implement an efficient nuclear security regime, and to figure out weak points. The IAEA's INPRO project suggests a checklist method for State's nuclear security regime. The IAEA is now researching more quantitative methods cooperatively with several countries including Korea. In this abstract, methodologies to evaluate state's nuclear security regime by risk assessment are addressed

  16. Oscillations of a highly discrete breather with a critical regime

    Science.gov (United States)

    Coquet; Remoissenet; Dinda

    2000-10-01

    We analyze carefully the essential features of the dynamics of a stationary discrete breather in the ultimate degree of energy localization in a nonlinear Klein-Gordon lattice with an on-site double-well potential. We demonstrate the existence of three different regimes of oscillatory motion in the breather dynamics, which are closely related to the motion of the central particle in an effective potential having two nondegenerate wells. In given parameter regions, we observe an untrapped regime, in which the central particle executes large-amplitude oscillations from one to the other side of the potential barrier. In other parameter regions, we find the trapped regime, in which the central particle oscillates in one of the two wells of the effective potential. Between these two regimes we find a critical regime in which the central particle undergoes several temporary trappings within an untrapped regime. Importantly, our study reveals that in the presence of purely anharmonic coupling forces, the breather compactifies, i.e., the energy becomes abruptly localized within the breather.

  17. 阿替普酶与尿激酶治疗急性脑梗死安全性的比较研究%THE STUDY ON SAFETY OF ALTEPLASE AND UROKINASE IN TREATMENT OF ACUTE CEREBRAL INFARCTION

    Institute of Scientific and Technical Information of China (English)

    鲍宇; 李国忠

    2015-01-01

    Objective To compare the safety of alteplase and urokinase in the treatment of acute cerebral infarc‐tion and to analyze the influencing factors of intracranial hemorrhage and death .Methods The data of 158 patients with acute cerebral infarction receiving thrombolytic therapy in Department of Neurology ,No .1 Affiliated Hospital of Harbin Medical University were colected .The patients were divided into the UK group of 105 patients and the Rt‐pa group of 53 patients based on the use of thrombolytic drugs .Conditions of patients with reinfarction ,hemor‐rhage and death were observed after thrombolysis .The influencing factors of hemorrhage and death were analyzed by logistic regression .Results In the UK group ,26 patients had bleeding complications ,13 patients got intracrani‐al hemorrhage ,and 4 patients suffered from symptomatic intracerebral hemorrhage .In the Rt‐pa group ,13 pa‐tients had bleeding complications ,6 patients got intracranial hemorrhage ,and no symptomatic intracranial hemor‐rhage occurred .After thrombolysis ,11 patients got reinfarction in the UK group ,9 patients got reinfarction in the Rt‐pa group .The deaths and were 11 in the UK group and 3 in the Rt‐pa group respectively .Logistic regression analysis showed that NIHSS score on admission was the dependent risk factor of cerebral hemorrhage and death . Conclusion There are no significant differences in the safety of urokinase and alteplase in the treatment of acute cer‐ebral infarction . NIHSS score on amdission is the independent factor of cerebral hemorrhage and death ,w hich indicates the higher the NIHSS scores are ,the greater the possibility of hemorrhage and death is .%目的:探索阿替普酶和尿激酶治疗急性脑梗死的安全性,以及溶栓后颅内出血、死亡的影响因素。方法选取哈尔滨医科大学附属第一医院神经内科静脉溶栓治疗的急性脑梗死患者158例,根据所用溶栓药物不同分为两组:U K

  18. Tumor marker utility and prognostic relevance of cathepsin B, cathepsin L, urokinase-type plasminogen activator, plasminogen activator inhibitor type-1, CEA and CA 19-9 in colorectal cancer

    International Nuclear Information System (INIS)

    Cathepsin B and L (CATB, CATL), urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 play an important role in colorectal cancer invasion. The tumor marker utility and prognostic relevance of these proteases have not been evaluated in the same experimental setting and compared with that of CEA and CA-19-9. Protease, CEA and CA 19-9 serum or plasma levels were determined in 56 patients with colorectal cancer, 25 patients with ulcerative colitis, 26 patients with colorectal adenomas and 35 tumor-free control patients. Protease, CEA, CA 19-9 levels have been determined by ELISA and electrochemiluminescence immunoassay, respectively; their sensitivity, specificity, diagnostic accuracy have been calculated and correlated with clinicopathological staging. The protease antigen levels were significantly higher in colorectal cancer compared with other groups. Sensitivity of PAI-1 (94%), CATB (82%), uPA (69%), CATL (41%) were higher than those of CEA or CA 19-9 (30% and 18%, respectively). PAI-1, CATB and uPA demonstrated a better accuracy than CEA or CA 19-9. A combination of PAI-1 with CATB or uPA exhibited the highest sensitivity value (98%). High CATB, PAI-1, CEA and CA 19-9 levels correlated with advanced Dukes stages. CATB (P = 0.0004), CATL (P = 0.02), PAI-1 (P = 0.01) and CA 19-9 (P = 0.004) had a significant prognostic impact. PAI-1 (P = 0.001), CATB (P = 0.04) and CA 19-9 (P = 0.02) proved as independent prognostic variables. At the time of clinical detection proteases are more sensitive indicators for colorectal cancer than the commonly used tumor markers. Determinations of CATB, CATL and PAI-1 have a major prognostic impact in patients with colorectal cancer

  19. A serpin-induced extensive proteolytic susceptibility of urokinase-type plasminogen activator implicates distortion of the proteinase substrate-binding pocket and oxyanion hole in the serpin inhibitory mechanism.

    Science.gov (United States)

    Egelund, R; Petersen, T E; Andreasen, P A

    2001-02-01

    The formation of stable complexes between serpins and their target serine proteinases indicates formation of an ester bond between the proteinase active-site serine and the serpin P1 residue [Egelund, R., Rodenburg, K.W., Andreasen, P.A., Rasmussen, M.S., Guldberg, R.E. & Petersen, T.E. (1998) Biochemistry 37, 6375-6379]. An important question concerning serpin inhibition is the contrast between the stability of the ester bond in the complex and the rapid hydrolysis of the acyl-enzyme intermediate in general serine proteinase-catalysed peptide bond hydrolysis. To answer this question, we used limited proteolysis to detect conformational differences between free urokinase-type plasminogen activator (uPA) and uPA in complex with plasminogen activator inhibitor-1 (PAI-1). Whereas the catalytic domain of free uPA, pro-uPA, uPA in complex with non-serpin inhibitors and anhydro-uPA in a non-covalent complex with PAI-1 was resistant to proteolysis, the catalytic domain of PAI-1-complexed uPA was susceptible to proteolysis. The cleavage sites for four different proteinases were localized in specific areas of the C-terminal beta-barrel of the catalytic domain of uPA, providing evidence that the serpin inhibitory mechanism involves a serpin-induced massive rearrangement of the proteinase active site, including the specificity pocket, the oxyanion hole, and main-chain binding area, rendering the proteinase unable to complete the normal hydrolysis of the acyl-enzyme intermediate. The distorted region includes the so-called activation domain, also known to change conformation on zymogen activation.

  20. Cadmium induces urokinase-type plasminogen activator receptor expression and the cell invasiveness of human gastric cancer cells via the ERK-1/2, NF-κB, and AP-1 signaling pathways.

    Science.gov (United States)

    Khoi, Pham Ngoc; Xia, Yong; Lian, Sen; Kim, Ho Dong; Kim, Do Hyun; Joo, Young Eun; Chay, Kee-Oh; Kim, Kyung Keun; Jung, Young Do

    2014-10-01

    Cadmium exposure has been linked to human cancers, including stomach cancer. In this study, the effects of cadmium on urokinase-type plasminogen activator receptor (uPAR) expression in human gastric cancer cells and the underlying signal transduction pathways were investigated. Cadmium induced uPAR expression in a time- and concentration-dependent manner. Cadmium also induced uPAR promoter activity. Additionally, cadmium induced the activation of extracellular signal regulated kinase-1/2 (ERK-1/2), p38 mitogen-activated protein kinase (MAPK), and the activation of c-Jun amino terminal kinase (JNK). A specific inhibitor of MEK-1 (PD98059) inhibited cadmium-induced uPAR expression, while JNK and p38 MAPK inhibitors did not. Expression vectors encoding dominant-negative MEK-1 (pMCL-K97M) also prevented cadmium-induced uPAR promoter activity. Site-directed mutagenesis and electrophoretic mobility shift studies showed that sites for the transcription factors nuclear factor (NF)-κB and activator protein-1 (AP-1) were involved in cadmium-induced uPAR transcription. Suppression of the cadmium-induced uPAR promoter activity by a mutated-type NF-κB-inducing kinase and I-κB and an AP-1 decoy oligonucleotide confirmed that the activation of NF-κB and AP-1 are essential for cadmium-induced uPAR upregulation. Cells pretreated with cadmium showed markedly enhanced invasiveness and this effect was partially abrogated by uPAR-neutralizing antibodies and by inhibitors of ERK-1/2, NF-κB, and AP-1. These results suggest that cadmium induces uPAR expression via ERK-1/2, NF-κB, and AP-1 signaling pathways and, in turn, stimulates cell invasiveness in human gastric cancer AGS cells.

  1. Stochastic Parametrisations and Regime Behaviour of Atmospheric Models

    Science.gov (United States)

    Arnold, Hannah; Moroz, Irene; Palmer, Tim

    2013-04-01

    The presence of regimes is a characteristic of non-linear, chaotic systems (Lorenz, 2006). In the atmosphere, regimes emerge as familiar circulation patterns such as the El-Nino Southern Oscillation (ENSO), the North Atlantic Oscillation (NAO) and Scandinavian Blocking events. In recent years there has been much interest in the problem of identifying and studying atmospheric regimes (Solomon et al, 2007). In particular, how do these regimes respond to an external forcing such as anthropogenic greenhouse gas emissions? The importance of regimes in observed trends over the past 50-100 years indicates that in order to predict anthropogenic climate change, our climate models must be able to represent accurately natural circulation regimes, their statistics and variability. It is well established that representing model uncertainty as well as initial condition uncertainty is important for reliable weather forecasts (Palmer, 2001). In particular, stochastic parametrisation schemes have been shown to improve the skill of weather forecast models (e.g. Berner et al., 2009; Frenkel et al., 2012; Palmer et al., 2009). It is possible that including stochastic physics as a representation of model uncertainty could also be beneficial in climate modelling, enabling the simulator to explore larger regions of the climate attractor including other flow regimes. An alternative representation of model uncertainty is a perturbed parameter scheme, whereby physical parameters in subgrid parametrisation schemes are perturbed about their optimal value. Perturbing parameters gives a greater control over the ensemble than multi-model or multiparametrisation ensembles, and has been used as a representation of model uncertainty in climate prediction (Stainforth et al., 2005; Rougier et al., 2009). We investigate the effect of including representations of model uncertainty on the regime behaviour of a simulator. A simple chaotic model of the atmosphere, the Lorenz '96 system, is used to study

  2. Positron acceleration in doughnut wakefields in the blowout regime

    Science.gov (United States)

    Vieira, Jorge; Mendonca, Jose; Fonseca, Ricardo; Silva, Luis

    2014-10-01

    Most important plasma acceleration results were reached in the so called bubble or blowout regime. Although ideally suited for electron acceleration, it has been recognized that non-linear regimes are not adequate to accelerate positrons. New configurations enabling positron acceleration in non-linear regimes would therefore open new research paths for future plasma based collider configurations. In this work, we explore, analytically and through 3D OSIRIS simulations, a novel configuration for positron acceleration in strongly non-linear laser wakefield excitation regimes using Laguerre-Gaussian laser drivers to drive doughnut shaped wakefields with positron focusing and accelerating fields. We demonstrate that positron focusing-fields can be up to an order of magnitude larger than electron focusing in the spherical blowout regime. The amplitude of the accelerating fields is similar to the spherical blowout. Simulations demonstrate laser self-guiding and stable positron acceleration until the laser energy has been exhausted to the plasma. Other realisations of the scheme, using two Gaussian laser pulses, will also be explored. FCT Grant No EXPL/FIS-PLA/0834/2012 and European Research Council ERC-2010-AdG Grant No. 267841.

  3. Early signatures of regime shifts in complex dynamical systems

    Indian Academy of Sciences (India)

    Indrani Bose; Mainak Pal

    2015-02-01

    A large number of studies have recently been carried out on the early signatures of regime shifts in a number of dynamical systems, e.g., ecosystems, the climate, fish and wildlife populations, financial markets, complex diseases and gene circuits. The underlying model in most cases is that of the fold-bifurcation in which a sudden regime shift occurs at a bifurcation point. The shift involves a discontinuous jump from one type of stable steady state to another. The dynamics of natural systems have both deterministic and stochastic components. The early signatures of abrupt regime shifts include the critical slowing down as a transition point is approached, rising variance and the lag-1 autocorrelation function, increased skewness of the steady-state probability distribution and the ratio of two mean first passage times for the exits from the stable steady states as the bifurcation point is approached. Noise-induced regime shifts are also possible for which the vicinity of the bifurcation point is not essential. In this paper, we review examples of regime shifts in natural systems and the associated early signatures. We further discuss how such approaches provide useful insights on a cell biological process involving the fold-bifurcation.

  4. Shear Capacity as Prognostic of Nocturnal Boundary Layer Regimes

    Science.gov (United States)

    van Hooijdonk, Ivo; Donda, Judith; Bosveld, Fred; Moene, Arnold; Clercx, Herman; van de Wiel, Bas

    2015-04-01

    After sunset the surface temperature can drop rapidly in some nights and may lead to ground frost. This sudden drop is closely related to the occurrence of fundamentally different behaviour of turbulence in the nocturnal boundary layer. Recent theoretical findings predict the appearance of two different regimes: the continuously turbulent (weakly stable) boundary layer and the relatively 'quiet' (very stable) boundary layer. Field observations from a large number of nights (approx. 4500 in total) are analysed using an ensemble averaging technique. The observations support the existence of these two fundamentally different regimes: weakly stable (turbulent) nights rapidly reach a steady state (within 2-3 hours). In contrast, very stable nights reach a steady state much later after a transition period (2-6 hours). During this period turbulence is weak and non-stationary. To characterise the regime a new parameter is introduced: the Shear Capacity. This parameter compares the actual shear after sunset with the minimum shear needed to sustain continuous turbulence. In turn, the minimum shear is dictated by the heat flux demand at the surface (net radiative cooling), so that the Shear Capacity combines flow information with knowledge on the boundary condition. It is shown that the Shear Capacity enables prediction of the flow regimes. The prognostic strength of this non-dimensional parameter appears to outperform the traditional ones like z/L and Ri as regime indicator.

  5. THE IMPACT OF THE GLOBAL NUCLEAR SAFETY REGIME IN BRAZIL

    International Nuclear Information System (INIS)

    A turning point of the world nuclear industry with respect to safety occurred due to the accident at Chernobyl, in 1986. A side from the tragic personal losses and the enormous financial damage, the Chernobyl accident has literally demonstrated that ''a nuclear accident anywhere is an accident everywhere''. The impact was felt immediately by the nuclear industry, with plant cancellations (e.g. Austria), elimination of national programs (e.g. Italy) and general construction delays. However, the reaction of the nuclear industry was equally immediate, which led to the proposal and establishment of a Global Nuclear Safety Regime. This regime is composed of biding international safety conventions, globally accepted safety standard, and a voluntary peer review system. In a previous work, the author has presented in detail the components of this Regime, and briefly discussed its impact in the Brazilian nuclear power organizations, including the Regulatory Body. This work, on the opposite, briefly reviews the Global Nuclear Safety Regime, and concentrates in detail in the discussion of its impact in Brazil, showing how it has produced some changes, and where the peer pressure regime has failed to produce real results

  6. Early Regimes of Water Capillary Flow in Slit Silica Nanochannels

    DEFF Research Database (Denmark)

    Oyarzua, Elton; Walther, Jens Honore; Mejia, Andres;

    2015-01-01

    Molecular dynamics simulations are conducted to investigate the initial stages of spontaneous imbibitionof water in slit silica nanochannels surrounded by air. An analysis is performed for the effects of nanoscopicconfinement, initial conditions of liquid uptake and air pressurization on the dyna......Molecular dynamics simulations are conducted to investigate the initial stages of spontaneous imbibitionof water in slit silica nanochannels surrounded by air. An analysis is performed for the effects of nanoscopicconfinement, initial conditions of liquid uptake and air pressurization...... on the dynamics of capillaryfilling. The results indicate that the nanoscale imbibition process is divided into three main flow regimes:an initial regime where the capillary force is balanced only by the inertial drag and characterized by aconstant velocity and a plug flow profile. In this regime, the meniscus...... formation process plays a centralrole in the imbibition rate. Thereafter, a transitional regime takes place, in which, the force balance hassignificant contributions from both inertia and viscous friction. Subsequently, a regime wherein viscousforces dominate the capillary force balance is attained. Flow...

  7. Hydrological minimal model for fire regime assessment in Mediterranean ecosystem

    Science.gov (United States)

    Ursino, N.; Rulli, M. C.

    2012-04-01

    A new model for Mediterranean forest fire regime assessment is presented and discussed. The model is based on the experimental evidence that fire is due to both hydrological and ecological processes and the relative role of fuel load versus fuel moisture is an important driver in fire ecology. Diverse scenarios are analyzed where either the hydrological forcing or the feedback between fire and hydrological characterization of the site is changed. The model outcome demonstrates that the two way interaction between hydrological processes, biology and fire regime drives the ecosystem toward a typical fire regime that may be altered either by an evolution of the biological characterization of the site or by a change of the hydrological forcing. This tenet implies that not every fire regime is compatible with the ecohydrological characterization of the site under study. This means that natural (non antropogenic) fire cannot be modeled as an arbitrary external forcing because the coupled hydrological and biological processes determines its statistical characterization, and conversely, the fire regime affects the soil moisture availability and the outcome of different species competition under possible water stress. The new modelling approach presented here, when provided by a proper model parameterization, can advance the capability in predicting and managing fires in ecosystems influenced by climate and land use changes.

  8. Soil magnetic susceptibility reflects soil moisture regimes and the adaptability of tree species to these regimes

    Science.gov (United States)

    Wang, J.-S.; Grimley, D.A.; Xu, C.; Dawson, J.O.

    2008-01-01

    Flooded, saturated or poorly drained soils are frequently anaerobic, leading to dissolution of the strongly magnetic minerals, magnetite and maghemite, and a corresponding decrease in soil magnetic susceptibility (MS). In this study of five temperate deciduous forests in east-central Illinois, USA, mean surface soil MS was significantly higher adjacent to upland tree species (31 ?? 10-5 SI) than adjacent to floodplain or lowland tree species (17 ?? 10-5 SI), when comparing regional soils with similar parent material of loessal silt. Although the sites differ in average soil MS for each tree species, the relative order of soil MS means for associated tree species at different locations is similar. Lowland tree species, Celtis occidentalis L., Ulmus americana L., Acer saccharinum L., Carya laciniosa (Michx. f.) Loud., and Fraxinus pennsylvanica Marsh. were associated with the lowest measured soil MS mean values overall and at each site. Tree species' flood tolerance rankings increased significantly, as soil MS values declined, the published rankings having significant correlations with soil MS values for the same species groups. The three published classifications of tree species' flood tolerance were significantly correlated with associated soil MS values at all sites, but most strongly at Allerton Park, the site with the widest range of soil drainage classes and MS values. Using soil MS measurements in forests with soil parent material containing similar initial levels of strongly magnetic minerals can provide a simple, rapid and quantitative method to classify soils according to hydric regimes, including dry conditions, and associated plant composition. Soil MS values thus have the capacity to quantify the continuum of hydric tolerances of tree species and guide tree species selection for reforestation. ?? 2007 Elsevier B.V. All rights reserved.

  9. A Markov Switching Regime Model of Malaysia Property Cycle

    Directory of Open Access Journals (Sweden)

    Abdul M. Beksin

    2011-01-01

    Full Text Available Problem statement: Non-linear models such as the Markov Switching regime (MS method of modelling business cycles, in principle can be used to model property cyle. Approach: The MS model can distinguish property cycle in recession and expansion phases and is sufficiently flexible to allow different relationships to apply over these phases. In this study, the Malaysian property cycle is modelled using a MS model. Results: This technique can be used to simultaneously estimate the data generating process of real GDP growth and classify each observation into one of two regimes (i.e., low-growth and high-growth regimes. Conclusions: This finding has important policy implications, since the yield spread was used to generate the time-varying probabilities of the MS model as well as the recession probabilities of the logit model. In other words, a strong relationship exists between interest rates and the business cycle, where interest rates lead the business cycle.

  10. Inhomogeneous stationary and oscillatory regimes in coupled chaotic oscillators.

    Science.gov (United States)

    Liu, Weiqing; Volkov, Evgeny; Xiao, Jinghua; Zou, Wei; Zhan, Meng; Yang, Junzhong

    2012-09-01

    The dynamics of linearly coupled identical Lorenz and Pikovsky-Rabinovich oscillators are explored numerically and theoretically. We concentrate on the study of inhomogeneous stable steady states ("oscillation death (OD)" phenomenon) and accompanying periodic and chaotic regimes that emerge at an appropriate choice of the coupling matrix. The parameters, for which OD occurs, are determined by stability analysis of the chosen steady state. Three model-specific types of transitions to and from OD are observed: (1) a sharp transition to OD from a nonsymmetric chaotic attractor containing random intervals of synchronous chaos; (2) transition to OD from the symmetry-breaking chaotic regime created by negative coupling; (3) supercritical bifurcation of OD into inhomogeneous limit cycles and further evolution of the system to inhomogeneous chaotic regimes that coexist with complete synchronous chaos. These results may fill a gap in the understanding of the mechanism of OD in coupled chaotic systems.

  11. Quantum SASE Regime for X-Ray FEL

    CERN Document Server

    Avetissian, H K

    2005-01-01

    For the X-ray FEL if the amplifying photon energy is larger or comparable to resonance widths caused by an electron beam spreads or by the finite interaction length then the quantum effects may play a significant role [1] and the generation process shifts from the classical to the quantum regime. The recent advancement of high brightness particle and laser beams technology makes achievable the fulfillment of these conditions in the scheme of X-ray Backscatter Compton laser. In this work we consider in general the SASE X-ray FEL in the quantum regime of amplification arising from the self-consistent set of the Maxwell and relativistic quantum kinetic equations. It is shown a considerable increase in start-up and narrowing of the spectrum of the SASE power for amplifying X-ray frequencies compared with the classical SASE regime.

  12. Dynamo regimes and transitions in the VKS experiment

    CERN Document Server

    Berhanu, M; Boisson, J; Gallet, B; Gissinger, C; Fauve, S; Mordant, N; Pétrélis, F; Bourgoin, M; Odier, P; Pinton, J -F; Plihon, N; Aumaître, S; Chiffaudel, A; Daviaud, F; Dubrulle, B; Pirat, C

    2015-01-01

    The Von K{\\'a}rm{\\'a}n Sodium experiment yields a variety of dynamo regimes, when asymmetry is imparted to the flow by rotating impellers at different speed F1 and F2. We show that as the intensity of forcing, measured as F1 + F2, is increased, the transition to a self-sustained magnetic field is always observed via a supercritical bifurcation to a stationary state. For some values of the asymmetry parameter $\\\\theta$ = (F1--F2)/(F1+F2), time dependent dynamo regimes develop. They are observed either when the forcing is increased for a given value of asymmetry, or when the amount of asymmetry is varied at sufficiently high forcing. Two qualitatively different transitions between oscillatory and stationary regimes are reported, involving or not a strong divergence of the period of oscillations. These transitions can be interpreted using a low dimensional model based on the interactions of two dynamo modes.

  13. Challenges in Finding AGNs in the Low Luminosity Regime

    Science.gov (United States)

    Satyapal, Shobita; Abel, Nick; Secrest, Nathan; Singh, Amrit; Ellison, Sara

    2016-08-01

    Low luminosity AGNs are an important component of the AGN population. They are often found in the lowest mass galaxies or galaxies that lack classical bulges, a demographic that places important constraints to models of supermassive black hole seed formation and merger-free models of AGN fueling. The detection of AGNs in this low luminosity regime is challenging both because star formation in the host galaxy can dominate the optical spectrum and gas and dust can obscure the central engine at both optical and X-ray wavelengths. Thus while mid-infrared color selection and X-ray observations at energies <10 keV are often powerful tools in uncovering optically unidentified AGNs at higher luminosities, this is not the case in the low luminosity regime. In this talk, I will review the effectiveness of uncovering AGNs in the low luminosity regime using multiwavength investigations, with a focus on infrared spectroscopic signatures.

  14. Nonlinear-optical frequency-doubling metareflector: pulsed regime

    Science.gov (United States)

    Popov, A. K.; Myslivets, S. A.

    2016-01-01

    The properties of backward-wave second-harmonic metareflector operating in pulse regime are investigated. It is made of metamaterial which enables phase matching of contra-propagating fundamental and second-harmonic waves. References are given to the works that prove such a possibility. Physical principles underlying differences in the proposed and standard settings as well as between continuous-wave and pulsed regimes are discussed. Pulsed regime is more practicable and has a broader scope of applications. A set of partial differential equations which describe such a reflector with the account for losses are solved numerically. It is shown that unlike second-harmonic generation in standard settings, contra-propagating pulse of second harmonic may become much longer than the incident fundamental one and the difference grows with decrease in the input pulse length as compared to thickness of the metaslab. The revealed properties are important for applications and may manifest themselves beyond the optical wavelength range.

  15. Adelie penguin foraging location predicted by tidal regime switching.

    Directory of Open Access Journals (Sweden)

    Matthew J Oliver

    Full Text Available Penguin foraging and breeding success depend on broad-scale environmental and local-scale hydrographic features of their habitat. We investigated the effect of local tidal currents on a population of Adélie penguins on Humble Is., Antarctica. We used satellite-tagged penguins, an autonomous underwater vehicle, and historical tidal records to model of penguin foraging locations over ten seasons. The bearing of tidal currents did not oscillate daily, but rather between diurnal and semidiurnal tidal regimes. Adélie penguins foraging locations changed in response to tidal regime switching, and not to daily tidal patterns. The hydrography and foraging patterns of Adélie penguins during these switching tidal regimes suggest that they are responding to changing prey availability, as they are concentrated and dispersed in nearby Palmer Deep by variable tidal forcing on weekly timescales, providing a link between local currents and the ecology of this predator.

  16. The political constitution of the EU citizen rights regime

    DEFF Research Database (Denmark)

    Olsen, Tore Vincents

    2011-01-01

    Reactions to decisions by the European Court of Justice (ECJ) demonstrate that the political institutions in the Union should take responsibility for the development of the structure of the European Union's (EU) citizen rights regime. This article analyses different political views on the EU...... citizen rights regime. It argues that the disagreement between them is largely a disagreement between ‘reasonable views’. The disagreement is mainly based on different views about the levels (European, national) at which individuals are to be seen as equals and about the contribution of different...... communities and institutions to the good life of citizens, both individually and collectively. Taking the contestation between the different views seriously, the article argues in favour of political constitutionalism, according to which the development of the EU citizen rights regime is the responsibility...

  17. Modeling whistler wave generation regimes in magnetospheric cyclotron maser

    Directory of Open Access Journals (Sweden)

    D. L. Pasmanik

    2004-11-01

    Full Text Available Numerical analysis of the model for cyclotron instability in the Earth's magnetosphere is performed. This model, based on the self-consistent set of equations of quasi-linear plasma theory, describes different regimes of wave generation and related energetic particle precipitation. As the source of free energy the injection of energetic electrons with transverse anisotropic distribution function to the interaction region is considered. A parametric study of the model is performed. The main attention is paid to the analysis of generation regimes for different characteristics of energetic electron source, such as the shape of pitch angle distributions and its intensity. Two mechanisms of removal of energetic electrons from a generation region are considered, one is due to the particle precipitation through the loss cone and another one is related to the magnetic drift of energetic particles.

    It was confirmed that two main regimes occur in this system in the presence of a constant particle source, in the case of precipitation losses. At small source intensity relaxation oscillations were found, whose parameters are in good agreement with simplified analytical theory developed earlier. At a larger source intensity, transition to a periodic generation occurs. In the case of drift losses the regime of self-sustained periodic generation regime is realized for source intensity higher than some threshold. The dependencies of repetition period and dynamic spectrum shape on the source parameters were studied in detail. In addition to simple periodic regimes, those with more complex spectral forms were found. In particular, alteration of spikes with different spectral shape can take place. It was also shown that quasi-stationary generation at the low-frequency band can coexist with periodic modulation at higher frequencies.

    On the basis of the results obtained, the model for explanation of

  18. How will climate change modify river flow regimes in Europe?

    Directory of Open Access Journals (Sweden)

    C. Schneider

    2013-01-01

    Full Text Available Worldwide, flow regimes are being modified by various anthropogenic impacts and climate change induces an additional risk. Rising temperatures, declining snow cover and changing precipitation patterns will interact differently at different locations. Consequently, in distinct climate zones, unequal consequences can be expected in matters of water stress, flood risk, water quality, and food security. In particular, river ecosystems and their vital ecosystem services will be compromised as their species richness and composition have evolved over long time under natural flow conditions. This study aims at evaluating the exclusive impacts of climate change on river flow regimes in Europe. Various flow characteristics are taken into consideration and diverse dynamics are identified for each distinct climate zone in Europe. In order to simulate present-day natural flow regimes and future flow regimes under climate change, the global hydrology model WaterGAP3 is applied. All calculations for current and future conditions (2050s are carried out on a 5' × 5' European grid. To address uncertainty, bias-corrected climate forcing data of three different global climate models are used to drive WaterGAP3. Finally, the hydrological alterations of different flow characteristics are quantified by the Indicators of Hydrological Alteration approach. Results of our analysis indicate that on the European scale, climate change can be expected to modify flow regimes remarkably. This is especially the case in the Mediterranean (due to drier conditions with reduced precipitation across the year and in the boreal climate zone (due to reduced snowmelt, increased precipitation, and strong temperature rises. In the temperate climate zone, impacts increase from oceanic to continental. Regarding single flow characteristics, strongest impacts on timing were found for the boreal climate zone. This applies for both high and low flows. Flow magnitudes, in turn, will be

  19. Terahertz Quantum Plasmonics of Nanoslot Antennas in Nonlinear Regime.

    Science.gov (United States)

    Kim, Joon-Yeon; Kang, Bong Joo; Park, Joohyun; Bahk, Young-Mi; Kim, Won Tae; Rhie, Jiyeah; Jeon, Hyeongtag; Rotermund, Fabian; Kim, Dai-Sik

    2015-10-14

    Quantum tunneling in plasmonic nanostructures has presented an interesting aspect of incorporating quantum mechanics into classical optics. However, the study has been limited to the subnanometer gap regime. Here, we newly extend quantum plasmonics to gap widths well over 1 nm by taking advantage of the low-frequency terahertz regime. Enhanced electric fields of up to 5 V/nm induce tunneling of electrons in different arrays of ring-shaped nanoslot antennas of gap widths from 1.5 to 10 nm, which lead to a significant nonlinear transmission decrease. These observations are consistent with theoretical calculations considering terahertz-funneling-induced electron tunneling across the gap.

  20. Selection Criteria in Regime Switching Conditional Volatility Models

    Directory of Open Access Journals (Sweden)

    Thomas Chuffart

    2015-05-01

    Full Text Available A large number of nonlinear conditional heteroskedastic models have been proposed in the literature. Model selection is crucial to any statistical data analysis. In this article, we investigate whether the most commonly used selection criteria lead to choice of the right specification in a regime switching framework. We focus on two types of models: the Logistic Smooth Transition GARCH and the Markov-Switching GARCH models. Simulation experiments reveal that information criteria and loss functions can lead to misspecification ; BIC sometimes indicates the wrong regime switching framework. Depending on the Data Generating Process used in the experiments, great care is needed when choosing a criterion.

  1. Phase contrast and operation regimes in multifrequency atomic force microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Sergio [Departament de Disseny i Programació de Sistemes Electrònics, UPC—Universitat Politècnica de Catalunya Av. Bases, 61, 08242 Manresa (Barcelona) (Spain)

    2014-04-07

    In amplitude modulation atomic force microscopy the attractive and the repulsive force regimes induce phase shifts above and below 90°, respectively. In the more recent multifrequency approach, however, multiple operation regimes have been reported and the theory should be revisited. Here, a theory of phase contrast in multifrequency atomic force microscopy is developed and discussed in terms of energy transfer between modes, energy dissipation and the kinetic energy and energy transfer associated with externally driven harmonics. The single frequency virial that controls the phase shift might undergo transitions in sign while the average force (modal virial) remains positive (negative)

  2. Dynamic two state stochastic models for ecological regime shifts

    DEFF Research Database (Denmark)

    Møller, Jan Kloppenborg; Carstensen, Niels Jacob; Madsen, Henrik;

    2009-01-01

    of the model illustrates that hysteresis effect and regime shifts can be obtained for a limited range of parameter values only. The effect of multiplicative noise components entering at different levels of the model is presented and discussed. Including noise leads to very different results on the stability...... of regimes, depending on how the noise propagates through the system. The dynamical properties of a system should therefore be described through propagation of the state distributions rather than the state means and consequently, stochastic models should be compared in a probabilistic framework....

  3. PREDICTION OF FLOW REGIMES IN SPOUT-FLUIDIZED BEDS

    Institute of Scientific and Technical Information of China (English)

    Jiyu Zhang; Fengxiang Tang

    2006-01-01

    Five main flow regimes in spout-fluidized bed were identified in this study, namely, fixed bed, spout with aeration, spout-fluidization, jet in fluidized bed and slugging, together with their corresponding major frequencies translated from pressure signals. The empirical equation A=aBb, in which A=Fr* /(H/Di) and B=(Fr*/(H/D))/(μg/μmf) are respectively the spout-geometry and spout-geometry-fluidization dimensionless numbers, was proposed to distinguish these flow regimes.

  4. NAZI REGIME IN THE PERIODICAL A NOTÍ­CIA.

    Directory of Open Access Journals (Sweden)

    Bruna Luíza Barcellos

    2009-08-01

    Full Text Available The periodical A Notícia, of Joinville, city colonized for Germans, supported the nazi regime since the ascension of Adolf Hitler to the power. Between 1932 and 1944, some editions had printed the figure of Hitler and the nazi symbol, beyond dither messages to that regime. Based in the Análise de conteúdo (BARDIN, 1977, this article searchs to understand the representation made for the periodical A Notícia on this historical landmark, in other words, bring to the light the consequences of a part of occured world-wide history in catarinense territory.

  5. Development and current trends in the international nonproliferation regime

    International Nuclear Information System (INIS)

    The IAEA and its safeguards system is placed into a historical perspective. A personal perspective on the nonproliferation regime and on the Agency's role in it is then presented. This is done initially by discussing some of the landmark events in the history of the nonproliferation regime. Subsequently some of the history of arms control agreements and of the role of the IAEA are noted. Then political motivations of state and ways the Agency has an impact in the political nonproliferation sphere are addressed

  6. Measurement of flow in supercritical flow regime using cutthroat flumes

    Indian Academy of Sciences (India)

    Shrikant A Tekade; Avinash D Vasudeo; Aniruddha D Ghare; Ramesh N Ingle

    2016-02-01

    Cutthroat flume is commonly used for measurement of subcritical flow in open channel because of its simplicity and ease of construction. No experimental data is available in literature for measurement of flow in supercritical regime using cutthroat flume. The present paper finds the feasibility of cutthroat flume as a measurement device for flow in supercritical regime. Experimental data are generated to develop the relation between discharge and observed head at a specified location on upstream of throat section. Regression analysis for discharge and head indicated a good correlation. Based on all the experimental data generated, a relationship between discharge and head is proposed.

  7. Explicit Mapping of Acoustic Regimes For Wind Instruments

    CERN Document Server

    Missoum, Samy; Doc, Jean-Baptiste

    2014-01-01

    This paper proposes a methodology to map the various acoustic regimes of wind instruments. The maps can be generated in a multi-dimensional space consisting of design, control parameters, and initial conditions. The bound- aries of the maps are obtained explicitly in terms of the parameters using a support vector machine (SVM) classifier as well as a dedicated adaptive sam- pling scheme. The approach is demonstrated on a simplified clarinet model for which several maps are generated based on different criteria. Examples of computation of the probability of occurrence of a specific acoustic regime are also provided. In addition, the approach is demonstrated on a design optimization example for optimal intonation.

  8. Using Clustering to Establish Climate Regimes from PCM Output

    Science.gov (United States)

    Oglesby, Robert; Arnold, James E. (Technical Monitor); Hoffman, Forrest; Hargrove, W. W.; Erickson, D.

    2002-01-01

    A multivariate statistical clustering technique--based on the k-means algorithm of Hartigan has been used to extract patterns of climatological significance from 200 years of general circulation model (GCM) output. Originally developed and implemented on a Beowulf-style parallel computer constructed by Hoffman and Hargrove from surplus commodity desktop PCs, the high performance parallel clustering algorithm was previously applied to the derivation of ecoregions from map stacks of 9 and 25 geophysical conditions or variables for the conterminous U.S. at a resolution of 1 sq km. Now applied both across space and through time, the clustering technique yields temporally-varying climate regimes predicted by transient runs of the Parallel Climate Model (PCM). Using a business-as-usual (BAU) scenario and clustering four fields of significance to the global water cycle (surface temperature, precipitation, soil moisture, and snow depth) from 1871 through 2098, the authors' analysis shows an increase in spatial area occupied by the cluster or climate regime which typifies desert regions (i.e., an increase in desertification) and a decrease in the spatial area occupied by the climate regime typifying winter-time high latitude perma-frost regions. The patterns of cluster changes have been analyzed to understand the predicted variability in the water cycle on global and continental scales. In addition, representative climate regimes were determined by taking three 10-year averages of the fields 100 years apart for northern hemisphere winter (December, January, and February) and summer (June, July, and August). The result is global maps of typical seasonal climate regimes for 100 years in the past, for the present, and for 100 years into the future. Using three-dimensional data or phase space representations of these climate regimes (i.e., the cluster centroids), the authors demonstrate the portion of this phase space occupied by the land surface at all points in space and time

  9. Using Clustering to Establish Climate Regimes from PCM Output

    Science.gov (United States)

    Hoffman, F.; Oglesby, R.; Hargrove, W. W.; Erickson, D.

    2002-12-01

    A multivariate statistical clustering technique--based on the k-means algorithm of Hartigan--has been used to extract patterns of climatological significance from 200 years of general circulation model (GCM) output. Originally developed and implemented on a Beowulf-style parallel computer constructed by Hoffman and Hargrove from surplus commodity desktop PCs, the high performance parallel clustering algorithm was previously applied to the derivation of ecoregions from map stacks of 9 and 25 geophysical conditions or variables for the conterminous U.S. at a resolution of 1 sq km. Now applied both across space and through time, the clustering technique yields temporally-varying climate regimes predicted by transient runs of the Parallel Climate Model (PCM). Using a business-as-usual (BAU) scenario and clustering four fields of significance to the global water cycle (surface temperature, precipitation, soil moisture, and snow depth) from 1871 through 2098, the authors' analysis shows an increase in spatial area occupied by the cluster or climate regime which typifies desert regions (i.e., an increase in desertification) and a decrease in the spatial area occupied by the climate regime typifying winter-time high latitude perma-frost regions. The patterns of cluster changes have been analyzed to understand the predicted variability in the water cycle on global and continental scales. In addition, representative climate regimes were determined by taking three 10-year averages of the fields 100 years apart for northern hemisphere winter (December, January, and February) and summer (June, July, and August). The result is global maps of typical seasonal climate regimes for 100 years in the past, for the present, and for 100 years into the future. Using three-dimensional data or phase space representations of these climate regimes (i.e., the cluster centroids), the authors demonstrate the portion of this phase space occupied by the land surface at all points in space and

  10. 2型糖尿病患者空腹和进食后2 h检验结果的比较研究%Comparative Study of the Examination Results under Fasting State and 2 Hours after Eating in Type 2 Diabetes

    Institute of Scientific and Technical Information of China (English)

    吕月

    2015-01-01

    目的:结合临床实践经验,对比2型糖尿病患者空腹状态下以及进食后2 h胰岛素浓度,分析2型糖尿病的发病机制,为其临床预后提供参考信息。方法研究对象取自于2013年3月—2014年12月该院接诊的80例2型糖尿病患者,全体患者均接受100 g馒头餐试验。分别测量患者空腹状态下以及进食后2h极胰岛素浓度,借助稳态模型计算β细胞功能及胰岛素抵抗指数。结果80例患者空腹状态以及进食后2 h血糖与HOMA-IR以及糖化血红蛋白呈正相关性,与HOM-β呈负相关性。相较于空腹状态,进食后2 h血糖浓度表现出明显的上升趋势,组间对比差异有统计学意义(P<0.05)。经pearson相关分析最终发现,两者间具有高度相关性。结论2型糖尿病患者用餐2 h后血糖异常,多与胰岛β细胞功能受损以及胰岛素抵抗有关,且进食后2h血糖水平对空腹血糖存在密切关联,临床务必对此给予高度重视。%Objective To compare the concentration of insulin under fasting state and 2 hours after eating in type 2 diabetes, and analyze the pathogenesis of type 2 diabetes based on the clinical practice so as to provide a reference for clinical prognosis of the disease. Methods 80 cases with type 2 diabetes admitted in our hospital from March 2013 to December 2014 were selected as the subjects. Eating 100g steamed bun test was conducted in all the subjects. And the concentration of insulin under fasting state and 2 hours after eating was measured respectively in the subjects. Steady-state model was used to calculate theβcells function and insulin resistance index. Results The fasting blood glucose and 2h postprandial blood glucose levels of the 80 subjects were positively correlated with HOMA-IR and glycosylated hemoglobin while those were negatively correlated with HOM-β. Compared with the fasting blood glucose level, the 2h postprandial blood glucose level increased more

  11. Predicting weather regime transitions in Northern Hemisphere datasets

    Energy Technology Data Exchange (ETDEWEB)

    Kondrashov, D. [University of California, Department of Atmospheric and Oceanic Sciences and Institute of Geophysics and Planetary Physics, Los Angeles, CA (United States); Shen, J. [UCLA, Department of Statistics, Los Angeles, CA (United States); Berk, R. [UCLA, Department of Statistics, Los Angeles, CA (United States); University of Pennsylvania, Department of Criminology, Philadelphia, PA (United States); D' Andrea, F.; Ghil, M. [Ecole Normale Superieure, Departement Terre-Atmosphere-Ocean and Laboratoire de Meteorologie Dynamique (CNRS and IPSL), Paris Cedex 05 (France)

    2007-10-15

    A statistical learning method called random forests is applied to the prediction of transitions between weather regimes of wintertime Northern Hemisphere (NH) atmospheric low-frequency variability. A dataset composed of 55 winters of NH 700-mb geopotential height anomalies is used in the present study. A mixture model finds that the three Gaussian components that were statistically significant in earlier work are robust; they are the Pacific-North American (PNA) regime, its approximate reverse (the reverse PNA, or RNA), and the blocked phase of the North Atlantic Oscillation (BNAO). The most significant and robust transitions in the Markov chain generated by these regimes are PNA {yields} BNAO, PNA {yields} RNA and BNAO {yields} PNA. The break of a regime and subsequent onset of another one is forecast for these three transitions. Taking the relative costs of false positives and false negatives into account, the random-forests method shows useful forecasting skill. The calculations are carried out in the phase space spanned by a few leading empirical orthogonal functions of dataset variability. Plots of estimated response functions to a given predictor confirm the crucial influence of the exit angle on a preferred transition path. This result points to the dynamic origin of the transitions. (orig.)

  12. Is the Economic Crisis Challenging the Prevailing Gender Regime?

    DEFF Research Database (Denmark)

    Leschke, Janine; Jepsen, Maria

    2014-01-01

    This paper addresses the question of whether the current economic and financial crisis is challenging the prevailing gender-equality model in four European countries: Denmark, Germany, Slovakia, and the United Kingdom. After situating the countries in relation to the underlying gender regime...

  13. Detection and Assessment of Ecosystem Regime Shifts from Fisher Information

    Directory of Open Access Journals (Sweden)

    B. Roy Frieden

    2008-06-01

    Full Text Available Ecosystem regime shifts, which are long-term system reorganizations, have profound implications for sustainability. There is a great need for indicators of regime shifts, particularly methods that are applicable to data from real systems. We have developed a form of Fisher information that measures dynamic order in complex systems. Here we propose the use of Fisher information as a means of: (1 detecting dynamic regime shifts in ecosystems, and (2 assessing the quality of the shift in terms of intensity and pervasiveness. Intensity is reflected by the degree of change in dynamic order, as determined by Fisher information, and pervasiveness is a reflection of how many observable variables are affected by the change. We present a new robust methodology to calculate Fisher information from time series field data. We demonstrate the use of Fisher information to detect regime shifts on a model for a shallow lake. Next, we use Fisher information to analyze marine ecosystem response to physical changes using real time-series data of a coastal marine ecosystem, the North Pacific Ocean.

  14. Exploiting synergies between nonproliferation verification regimes: A pragmatic approach

    International Nuclear Information System (INIS)

    This paper considers the potential for expanding interaction and exploiting synergies between different multilateral disarmament and nonproliferation regimes. It concludes that although there are political barriers to high-level and pervasive synergization, there are opportunities for pragmatic, functional steps to be taken as a way of building confidence and experience. (author)

  15. Dynamic Financial Constraints: Distinguishing Mechanism Design from Exogenously Incomplete Regimes.

    Science.gov (United States)

    Karaivanov, Alexander; Townsend, Robert M

    2014-05-01

    We formulate and solve a range of dynamic models of constrained credit/insurance that allow for moral hazard and limited commitment. We compare them to full insurance and exogenously incomplete financial regimes (autarky, saving only, borrowing and lending in a single asset). We develop computational methods based on mechanism design, linear programming, and maximum likelihood to estimate, compare, and statistically test these alternative dynamic models with financial/information constraints. Our methods can use both cross-sectional and panel data and allow for measurement error and unobserved heterogeneity. We estimate the models using data on Thai households running small businesses from two separate samples. We find that in the rural sample, the exogenously incomplete saving only and borrowing regimes provide the best fit using data on consumption, business assets, investment, and income. Family and other networks help consumption smoothing there, as in a moral hazard constrained regime. In contrast, in urban areas, we find mechanism design financial/information regimes that are decidedly less constrained, with the moral hazard model fitting best combined business and consumption data. We perform numerous robustness checks in both the Thai data and in Monte Carlo simulations and compare our maximum likelihood criterion with results from other metrics and data not used in the estimation. A prototypical counterfactual policy evaluation exercise using the estimation results is also featured.

  16. Just for Fun? The Emotional Regime of Experiential Consumption

    DEFF Research Database (Denmark)

    Jantzen, Christian; Fitchett, James; Østergaard, Per;

    2012-01-01

    Experiential consumption emphasizes emotional and hedonic qualities in the marketplace stressing the importance of experiences for ‘the good life’ and positioning consumption as a legitimate way to generate interesting and relevant experiences. The concept of emotional regimes (Reddy, 2001) is used...

  17. Process-based humidity control regime for greenhouse crops

    NARCIS (Netherlands)

    Korner, O.; Challa, H.

    2003-01-01

    Modern greenhouses in The Netherlands are designed for efficient use of energy. Climate control traditionally aims at optimal crop performance. However, energy saving is a major issue for the development of new temperature regimes. Temperature integration (TI) results in fluctuating and often high r

  18. Operating regimes of signaling cycles: statics, dynamics, and noise filtering.

    Directory of Open Access Journals (Sweden)

    Carlos Gomez-Uribe

    2007-12-01

    Full Text Available A ubiquitous building block of signaling pathways is a cycle of covalent modification (e.g., phosphorylation and dephosphorylation in MAPK cascades. Our paper explores the kind of information processing and filtering that can be accomplished by this simple biochemical circuit. Signaling cycles are particularly known for exhibiting a highly sigmoidal (ultrasensitive input-output characteristic in a certain steady-state regime. Here, we systematically study the cycle's steady-state behavior and its response to time-varying stimuli. We demonstrate that the cycle can actually operate in four different regimes, each with its specific input-output characteristics. These results are obtained using the total quasi-steady-state approximation, which is more generally valid than the typically used Michaelis-Menten approximation for enzymatic reactions. We invoke experimental data that suggest the possibility of signaling cycles operating in one of the new regimes. We then consider the cycle's dynamic behavior, which has so far been relatively neglected. We demonstrate that the intrinsic architecture of the cycles makes them act--in all four regimes--as tunable low-pass filters, filtering out high-frequency fluctuations or noise in signals and environmental cues. Moreover, the cutoff frequency can be adjusted by the cell. Numerical simulations show that our analytical results hold well even for noise of large amplitude. We suggest that noise filtering and tunability make signaling cycles versatile components of more elaborate cell-signaling pathways.

  19. Accessing Imagined Communities and Reinscribing Regimes of Truth

    Science.gov (United States)

    Carroll, Sherrie; Motha, Suhanthie; Price, Jeremy N.

    2008-01-01

    In this article, we explore the complex and nebulous terrain between two theoretical concepts, imagined communities (Norton, 2000, 2001), that is, individuals' imagined affiliations with certain groups, and regimes of truth (Foucault, 1980), dominant images inscribed and reinscribed into individual consciousness until they become normative. Using…

  20. Regimes of flow induced vibration for tandem, tethered cylinders

    Science.gov (United States)

    Nave, Gary; Stremler, Mark

    2015-11-01

    In the wake of a bluff body, there are a number of dynamic response regimes that exist for a trailing bluff body depending on spacing, structural restoring forces, and the mass-damping parameter m* ζ . For tandem cylinders with low values of m* ζ , two such regimes of motion are Gap Flow Switching and Wake Induced Vibration. In this study, we consider the dynamics of a single degree-of-freedom rigid cylinder in the wake of another in these regimes for a variety of center-to-center cylinder spacings (3-5 diameters) and Reynolds numbers (4,000-11,000). The system consists of a trailing cylinder constrained to a circular arc around a fixed leading cylinder, which, for small angle displacements, bears a close resemblance to the transversely oscillating cylinders found more commonly in existing literature. From experiments on this system, we compare and contrast the dynamic response within these two regimes. Our results show sustained oscillations in the absence of a structural restoring force in all cases, providing experimental support for the wake stiffness assumption, which is based on the mean lift toward the center line of flow.

  1. Exploring the epsilon regime with twisted mass fermions

    CERN Document Server

    Jansen, K; Shindler, A; Urbach, C; Wenger, U

    2007-01-01

    In this proceeding contribution we report on a first study in order to explore the so called epsilon regime with Wilson twisted mass (Wtm) fermions. To show the potential of this approach we give a preliminary determination of the chiral condensate.

  2. Dynamic Financial Constraints: Distinguishing Mechanism Design from Exogenously Incomplete Regimes.

    Science.gov (United States)

    Karaivanov, Alexander; Townsend, Robert M

    2014-05-01

    We formulate and solve a range of dynamic models of constrained credit/insurance that allow for moral hazard and limited commitment. We compare them to full insurance and exogenously incomplete financial regimes (autarky, saving only, borrowing and lending in a single asset). We develop computational methods based on mechanism design, linear programming, and maximum likelihood to estimate, compare, and statistically test these alternative dynamic models with financial/information constraints. Our methods can use both cross-sectional and panel data and allow for measurement error and unobserved heterogeneity. We estimate the models using data on Thai households running small businesses from two separate samples. We find that in the rural sample, the exogenously incomplete saving only and borrowing regimes provide the best fit using data on consumption, business assets, investment, and income. Family and other networks help consumption smoothing there, as in a moral hazard constrained regime. In contrast, in urban areas, we find mechanism design financial/information regimes that are decidedly less constrained, with the moral hazard model fitting best combined business and consumption data. We perform numerous robustness checks in both the Thai data and in Monte Carlo simulations and compare our maximum likelihood criterion with results from other metrics and data not used in the estimation. A prototypical counterfactual policy evaluation exercise using the estimation results is also featured. PMID:25246710

  3. Dynamic Financial Constraints: Distinguishing Mechanism Design from Exogenously Incomplete Regimes*

    Science.gov (United States)

    Karaivanov, Alexander; Townsend, Robert M.

    2014-01-01

    We formulate and solve a range of dynamic models of constrained credit/insurance that allow for moral hazard and limited commitment. We compare them to full insurance and exogenously incomplete financial regimes (autarky, saving only, borrowing and lending in a single asset). We develop computational methods based on mechanism design, linear programming, and maximum likelihood to estimate, compare, and statistically test these alternative dynamic models with financial/information constraints. Our methods can use both cross-sectional and panel data and allow for measurement error and unobserved heterogeneity. We estimate the models using data on Thai households running small businesses from two separate samples. We find that in the rural sample, the exogenously incomplete saving only and borrowing regimes provide the best fit using data on consumption, business assets, investment, and income. Family and other networks help consumption smoothing there, as in a moral hazard constrained regime. In contrast, in urban areas, we find mechanism design financial/information regimes that are decidedly less constrained, with the moral hazard model fitting best combined business and consumption data. We perform numerous robustness checks in both the Thai data and in Monte Carlo simulations and compare our maximum likelihood criterion with results from other metrics and data not used in the estimation. A prototypical counterfactual policy evaluation exercise using the estimation results is also featured. PMID:25246710

  4. Political Regime and Human Capital: A Cross-Country Analysis

    Science.gov (United States)

    Klomp, Jeroen; de Haan, Jakob

    2013-01-01

    We examine the relationship between different dimensions of the political regime in place and human capital using a two-step structural equation model. In the first step, we employ factor analysis on 16 human capital indicators to construct two new human capital measures (basic and advanced human capital). In the second step, we estimate the…

  5. Political regime and human capital : A cross-country analysis

    NARCIS (Netherlands)

    Klomp, J.G.; de Haan, J.

    2013-01-01

    We examine the relationship between different dimensions of the political regime in place and human capital using a two-step structural equation model. In the first step, we employ factor analysis on 16 human capital indicators to construct two new human capital measures (basic and advanced human ca

  6. Political regime and human capital: A cross-country analysis

    NARCIS (Netherlands)

    Klomp, J.G.; Haan, de J.

    2013-01-01

    We examine the relationship between different dimensions of the political regime in place and human capital using a two-step structural equation model. In the first step, we employ factor analysis on 16 human capital indicators to construct two new human capital measures (basic and advanced human ca

  7. Syria: The Consolidation of the Asad Regime, 1970-1975.

    Science.gov (United States)

    Olson, Robert W.

    1982-01-01

    Up to 1975-76, Syria's Hafiz al-Asad was successful in consolidating his power, securing the maintenance of his regime, and broadening his base of political power. His system started weakening in 1975 due to increased opposition stemming from Syria's intervention in the Lebanese civil war. (AM)

  8. Shearing box simulations in the Rayleigh unstable regime

    DEFF Research Database (Denmark)

    Nauman, Farrukh; Blackman, Eric G.

    2015-01-01

    We study the stability properties of Rayleigh unstable flows both in the purely hydrodynamic and magnetohydrodynamic (MHD) regimes for two different values of the shear $q=2.1, 4.2$ ($q = - d\\ln\\Omega / d\\ln r$) and compare it with the Keplerian case $q=1.5$. The Rayleigh stability criterion states...

  9. Welfare Attitudes and Social Expenditure: Do Regimes Shape Public Opinion?

    Science.gov (United States)

    Jakobsen, Tor Georg

    2011-01-01

    This article examines the link between regime types, social expenditure, and welfare attitudes. By employing data on 19 countries taken from the World Values Survey, the main aim is to see to what degree the institutions of a country affect the attitudes of its citizens. According to Esping-Andersen ("The three worlds of welfare capitalism".…

  10. Alternative pricing regimes in Ontario : exploring the impacts

    International Nuclear Information System (INIS)

    Legislative goals were recently established in Ontario to promote reliability and quality of electricity service and to ensure that distribution rates for customers remain reasonable. This presentation explored the effect of changing electricity pricing structures on residential customers in Ontario. This study investigated a period between May to December 2005, in the town of Milton, Ontario. Monthly demand was measured for each month, and monthly weighted averages were presented. Residents with electric heating were removed from the sample. Four pricing structure scenarios were examined: (1) flat rates; (2) time-of-use pricing regimes; (3) real time pricing regimes; and (4) critical peak pricing. Average monthly consumption rates for July and August for all 4 scenarios were presented. Results for time-of-use were compared to flat rates, which showed a slight increase in monthly costs. Real time average monthly electricity costs were significantly higher. Time-of-use costs increased by 57 per cent during the periods examined. Real time pricing regimes resulted in a 196 per cent rise in costs. It was concluded that more research must be done to explore the policy implications of pricing regimes and their effect on consumer behaviour. refs., tabs., figs

  11. Towards an Orderly Exit Regime in English Higher Education

    Science.gov (United States)

    Fisher, Andrew

    2016-01-01

    In a competitive market, the exit of those suppliers who cannot offer an attractive product at an attractive price is seen as desirable. However, the consequences for consumers when their own supplier leaves the market in an unplanned or disorderly way may be undesirable. Exit regimes exist in regulated markets to ensure that consumers are not…

  12. Oil and gas fiscal regimes of the western Canadian provinces

    International Nuclear Information System (INIS)

    This report compares the fiscal regimes in British Columbia, Alberta, Saskatchewan and Manitoba. During 1985-1988, federal and provincial governments have made numerous fiscal changes, many in response to the drop in world oil prices. The new fiscal policies generally have reflected governments' willingness to forego revenues in an effort to aid the oil and gas industry, with certain exemptions. Since 1988, changes have reflected trends of consolidation and less government willingness to forego revenues. A federal large corporations capital tax has been introduced, the natural gas exploration holiday in Alberta expired, new oil royalties were introduced, and changes were made in fiscal regimes to accomodate horizontal drilling in Saskatchewan and Manitoba. In this document, the existing corporate tax regime is described. A comparison of fiscal regimes must recognize the differing scale and nature of oil and gas operations among the 4 provinces, with Alberta accounting for 80-90% of Canada's oil and gas productions, while British Columbia, Saskatchewan and Manitoba are much smaller producers. The document describes Crown royalties and incentives and freehold taxes for each type of fuel (crude oil, natural gas, natural gas byproducts, nonconventional oil). 8 figs

  13. Nonlinear regimes in mean-field full-sphere dynamo

    CERN Document Server

    Pipin, V V

    2016-01-01

    The mean-field dynamo model is employed to study the non-linear dynamo regimes in a fully convective star of mass 0.3$M_{\\odot}$ rotating with period of 10 days. The differential rotation law was estimated using the mean-field hydrodynamic and heat transport equations. For the intermediate parameter of the turbulent magnetic Reynolds number, $Pm_{T}=3$ we found the oscillating dynamo regimes with period about 40Yr. The higher $Pm_{T}$ results to longer dynamo periods. The meridional circulation has one cell per hemisphere. It is counter-clockwise in the Northen hemisphere. The amplitude of the flow at the surface around 1 m/s. Tne models with regards for meridional circulation show the anti-symmetric relative to equator magnetic field. If the large-scale flows is fixed we find that the dynamo transits from axisymmetric to non-axisymmetric regimes for the overcritical parameter of the $\\alpha$effect. The change of dynamo regime occurs because of the non-axisymmetric non-linear $\\alpha$-effect. The situation pe...

  14. Microgravity Flow Regime Data: Buoyancy and Mixing Apparatus Effects

    Science.gov (United States)

    Shephard, Adam; Best, Frederick

    2010-01-01

    Zero-g two-phase flow data set qualification and flight experiment design have not been standardized and as a result, agreement among researchers has not been reached regarding what experimental conditions adequately approximate those of microgravity. The effects of buoyancy forces and mixing apparatus on the flow regime transitions are presented in this study. The gravity conditions onboard zero-g aircraft are at best 10-3 g which is used to approximate the 10-5 g conditions of microgravity, thus the buoyancy forces present on zero-g aircraft can become significantly large and unrepresentative of microgravity. When buoyancy forces approach those of surface tension forces, buoyancy induced coalescence occurs. When discussing flow regime transitions, these large buoyancy forces lead to flow regime transitions which otherwise would not occur. The buoyancy attributes of the two-phase flow data sets available in the literature are evaluated to determine which data sets exhibit buoyancy induced transitions. Upon comparison of the representative data sets, the affects of different mixing apparatus can be seen in the superficial velocity flow regime maps.

  15. Sputtering of Ge(001): transition between dynamic scaling regimes

    DEFF Research Database (Denmark)

    Smilgies, D.-M.; Eng, P.J.; Landemark, E.;

    1997-01-01

    We have studied the dynamic behavior of the Ge(001) surface during sputtering in situ and in real time using synchrotron X-ray diffraction. We find two dynamic regimes as a function of surface temperature and sputter current which are separated by a sharp transition. The boundary between these two...

  16. Search Regimes and the Industrial Dynamics of Science

    Science.gov (United States)

    Bonaccorsi, Andrea

    2008-01-01

    The article addresses the issue of dynamics of science, in particular of new sciences born in twentieth century and developed after the Second World War (information science, materials science, life science). The article develops the notion of search regime as an abstract characterization of dynamic patterns, based on three dimensions: the rate of…

  17. Regime shifts limit the predictability of land-system change

    DEFF Research Database (Denmark)

    Müller, Daniel; Sun, Zhanli; Vongvisouk, Thoumthone;

    2014-01-01

    (China, Laos, Vietnam and Indonesia). The results show how sudden events and gradual changes in underlying drivers caused rapid, surprising and widespread land-system changes, including shifts to different regimes in China, Vietnam and Indonesia, whereas land systems in Laos remained stable in the study...

  18. Globalisation, the "Idea of a University" and Its Ethical Regimes

    Science.gov (United States)

    Marginson, Simon

    2007-01-01

    This paper sketches the impact of globalisation and internationalisation on the terrain of values and ethics in higher education. The first part of the paper discusses values and ethics in higher education in relation to the "Idea of a University", and identifies the ethical regimes essential to the functioning of HEIs as knowledge-forming…

  19. Morphodynamic regime and long-term evolution of meandering rivers

    Science.gov (United States)

    Frascati, Alessandro; Lanzoni, Stefano

    2009-06-01

    In the present contribution we focus our attention on the long-term behavior of meandering rivers, a very common pattern in nature. This class of dynamical systems is driven by the coexistence of various intrinsically nonlinear mechanisms which determine the possible occurrence of two different morphodynamic regimes: the subresonant and the superresonant regimes. Investigating the full range of morphodynamic conditions, we objectively compare the morphologic characteristics exhibited by synthetically generated and observed planimetric patterns. The analysis is carried out examining, through principal component analysis, a suitable set of morphological variables. We show that even in the presence of the strong filtering action exerted by cutoff processes, a closer, although not yet complete, similarity with natural meandering planforms can be achieved only by adopting a flow field model which accounts for the full range of morphodynamic regimes. We also introduce a new morphodynamic length scale, ?m, associated with spatially oscillating disturbances. Once normalized with this length scale, the relevant morphologic features of the simulated long-term patterns (e.g., the probability density function of local curvature and the geometric characteristics of oxbow lakes) tend to collapse on two distinct behaviors, depending on the dominant morphologic regime.

  20. Analysis of the Two-Regime Method on Square Meshes

    KAUST Repository

    Flegg, Mark B.

    2014-01-01

    The two-regime method (TRM) has been recently developed for optimizing stochastic reaction-diffusion simulations [M. Flegg, J. Chapman, and R. Erban, J. Roy. Soc. Interface, 9 (2012), pp. 859-868]. It is a multiscale (hybrid) algorithm which uses stochastic reaction-diffusion models with different levels of detail in different parts of the computational domain. The coupling condition on the interface between different modeling regimes of the TRM was previously derived for onedimensional models. In this paper, the TRM is generalized to higher dimensional reaction-diffusion systems. Coupling Brownian dynamics models with compartment-based models on regular (square) two-dimensional lattices is studied in detail. In this case, the interface between different modeling regimes contains either flat parts or right-angle corners. Both cases are studied in the paper. For flat interfaces, it is shown that the one-dimensional theory can be used along the line perpendicular to the TRM interface. In the direction tangential to the interface, two choices of the TRM parameters are presented. Their applicability depends on the compartment size and the time step used in the molecular-based regime. The two-dimensional generalization of the TRM is also discussed in the case of corners. © 2014 Society for Industrial and Applied Mathematics.

  1. The perturbative Regge-calculus regime of loop quantum gravity

    Energy Technology Data Exchange (ETDEWEB)

    Bianchi, Eugenio [Scuola Normale Superiore and INFN Pisa, Piazza dei Cavalieri 7, I-56126 Pisa (Italy)], E-mail: e.bianchi@sns.it; Modesto, Leonardo [Department of Physics, Bologna University and INFN Bologna, V. Irnerio 46, I-40126 Bologna (Italy); Centre de Physique Theorique de Luminy, Universite de la Mediterranee, F-13288 Marseille (France)], E-mail: modesto@bo.infn.it

    2008-06-21

    The relation between loop quantum gravity and Regge calculus has been pointed out many times in the literature. In particular the large spin asymptotics of the Barrett-Crane vertex amplitude is known to be related to the Regge action. In this paper we study a semiclassical regime of loop quantum gravity and show that it admits an effective description in terms of perturbative area-Regge-calculus. The regime of interest is identified by a class of states given by superpositions of four-valent spin networks, peaked on large spins. As a probe of the dynamics in this regime, we compute explicitly two- and three-area correlation functions at the vertex amplitude level. We find that they match with the ones computed perturbatively in area-Regge-calculus with a single 4-simplex, once a specific perturbative action and measure have been chosen in the Regge-calculus path integral. Correlations of other geometric operators and the existence of this regime for other models for the dynamics are briefly discussed.

  2. Second regime tokamak operation at large aspect ratio

    International Nuclear Information System (INIS)

    The equilibrium, stability, and transport properties of large aspect ratio tokamaks operating at the second stability regime are described theoretically using numerical and analytical techniques. It has been shown that, at large aspect ratio, significant current profile control is possible with relatively modest amounts of neutral beam current drive, and the power needed to access and maintain the second regime operation is calculated to be about 3 MW using the results of an integrated 1.5D transport and stability code. An example second regime experiment has been described and the results are presented of extensive calculations illustrating several possible operating scenarios, external and internal model stability boundaries, and the experimental features needed to evaluate and test the high beta tokamak theories. The theory which describes the stabilizing effect of energetic particles during high beta operation was extended to finite aspect ratio. A key technical problem for application of this technique appears to be caused by ripple transport. Plasma rotation effects are found to be generally destabilizing and several other schemes for improved access to the second stability regime are discussed including ponderomotive stabilization of the plasma edge region and active feedback control

  3. Learning, competency nomads, and post-signifying regimes

    DEFF Research Database (Denmark)

    Krejsler, John

    2007-01-01

    of these changes as a transition from ‘industrial' to ‘knowledge society'; this is done by re-describing this transition from within as a transition from ‘signifying' to ‘post-signifying' regimes. From this conceptual experiment the ‘competency nomad' emerges as a powerful concept that seems useful to replace...

  4. Perfect photon absorption in nonlinear regime of cavity quantum electrodynamics

    CERN Document Server

    Agarwal, G S; Wang, Liyong; Zhu, Yifu

    2016-01-01

    It has been shown that perfect photon absorption can occur in the linear excitation regime of cavity quantum electrodynamics (CQED), in which photons from two identical light fields coupled into two ends of the cavity are completely absorbed and result in excitation of the polariton state of the CQED system. The output light from the cavity is totally suppressed by the destructive interference and the polariton state can only decay incoherently back to the ground state. Here we analyze the perfect photon absorption and onset of optical bistability in the nonlinear regime of the CQED and show that the perfect photon absorption persists in the nonlinear regime of the CQED below the threshold of the optical bistability. Therefore the perfect photon absorption is a phenomenon that can be observed in both linear and nonlinear regimes of CQED. Furthermore, our study reveals for the first time that the optical bistability is influenced by the input-light interference and can be manipulated by varying the relative ph...

  5. Primordial black holes in linear and non-linear regimes

    CERN Document Server

    Allahyari, Alireza; Abolhasani, Ali Akbar

    2016-01-01

    Using the concept of apparent horizon for dynamical black holes, we revisit the formation of primordial black holes (PBH) in the early universe for both linear and non-linear regimes. First, we develop the perturbation theory for spherically symmetric spacetimes to study the formation of spherical PBHs in linear regime and we fix two gauges. We also introduce a well defined gauge invariant quantity for the expansion. Using this quantity, we argue that PBHs do not form in the linear regime. Finally, we study the non-linear regime. We adopt the spherical collapse picture by taking a closed FRW model in the radiation dominated era to investigate PBH formation. Taking the initial condition of the spherical collapse from the linear theory of perturbations, we allow for both density and velocity perturbations. Our model gives a constraint on the velocity perturbation. This model also predicts that the apparent horizon of PBHs forms when $\\delta > 3$. Applying the sound horizon constraint, we have shown the threshol...

  6. Regime-switching models to study psychological process

    NARCIS (Netherlands)

    Hamaker, E.L.; Grasman, R.P.P.P.; Kamphuis, J.H.

    2010-01-01

    Many psychological processes are characterized by recurrent shifts between different states. To model these processes at the level of the individual, regime-switching models may prove useful. In this chapter we discuss two of these models: the threshold autoregressive model and the Markov switching

  7. 急性高危肺栓塞介入取栓、溶栓中应用 r-tPA 与尿激酶溶栓时间及疗效比较%Comparison of curative effect and thrombolysis time between r-tPA application and urokinase in the interventional thrombectomy and thrombolysis for acute and severe pulmonary embolism

    Institute of Scientific and Technical Information of China (English)

    冯琦琛; 李选; 董国祥; 傅军; 王昌明

    2014-01-01

    Objective:To study the influence of r-tPA and urokinase in curative effect and thrombolysis time on patients with acute and severe pulmonary embolism after interventional thrombectomy .Methods:After reviewing and analyzing the clinical data of 19 acute and severe pulmonary embolism patients , we classified them into two groups in accordance with the application of r-tPA and urokinase to compare the changes of their heart rate , blood pressure , pulmonary arterial pressure , arterial partial pressure of oxygen and hemachrome before and after thrombolysis , as well as the thrombolysis effect time the two groups took.Results: The heart rate, blood pressure, pulmonary arterial pressure , arterial partial pressure of oxygen of the patients in both groups gained remarkable improvement after operation ( P0.05); the pulmonary arterial pressure of the urokinase patient group dropped significantly compared with that study before operation ( P0 .05 );the thrombolysis effect time by adop-ting r-tPA was remarkably shorter than that caused in thrombolysis by adopting urokinase ( P<0 .05 ) . Conclusion: Both r-tPA and urokinase are effective in interventional thrombolysis for acute and severe pulmonary embolism .However , r-tPA could significantly shorten the time caused in thrombolysis without increasing any bleeding risk.%目的:探讨急性高危型肺栓塞患者经介入取栓后,应用r-tPA和尿激酶行介入溶栓治疗,对疗效和溶栓时间的影响。方法:回顾性分析19例急性高危型肺栓塞患者的临床资料,根据其应用r-tPA或尿激酶行介入溶栓分为两组,比较两组间溶栓前后心率、血压、肺动脉压、动脉血氧分压和血红蛋白的变化,以及两组的溶栓时间。结果:各组患者术前和术后心率、血压、动脉血氧分压均有明显改善(P<0.05);应用r-tPA组患者术后肺动脉压较术前有所降低,但差异无统计学意义(P>0.05),应用尿激酶组患者术

  8. Clinical curative effect observation of continuous transfusion of heparin sodium combined with urokinase and warfarin by micropump in the treatment of deep venous thrombosis%微量泵持续输注肝素钠联合尿激酶、华法林治疗下肢深静脉血栓形成的临床效果观察

    Institute of Scientific and Technical Information of China (English)

    赵晓玲; 王明; 陈建明

    2016-01-01

    Objective:To investigate the clinical curative effect of continuous transfusion of heparin sodium combined with urokinase and warfarin by micropump in the treatment of deep venous thrombosis.Methods:86 cases of patients with deep venous thrombosis were divided into two groups randomly.The treatment group was treated with continuous transfusion of heparin sodium combined with urokinase and warfarin by micropump on the basis of conventional treatment,the control group was treated with urokinase,warfarin and low molecular heparin calcium on the basis of conventional treatment,the cure rate and effective rate of the two groups were compared.Results:The cure rate of the treatment group was 72.5%,and the total effective rate was 89.7%.The cure rate of the control group was 60.2%,and the total effective rate was 78.9%.The difference of clinical curative effect between the two groups was statistically significant(P<0.05).No serious bleeding events occurred in the two groups.Conclusion:The clinical curative effect of continuous transfusion of heparin sodium combined with urokinase and warfarin by micropump in the treatment of deep venous thrombosis was significantly better than that of low molecular heparin calcium combined with urokinase and warfarin,and the bleeding events were not significantly increased.%目的:探讨微量泵持续输注肝素钠联合尿激酶、华法林治疗下肢深静脉血栓形成的临床效果.方法:收治下肢深静脉血栓形成患者86例,随机分为两组.治疗组在常规治疗基础上,以微量泵持续输注肝素钠联合尿激酶、华法林,对照组在常规治疗基础上给予尿激酶、华法林、低分子肝素钙,比较两组治愈率及有效率.结果:治疗组治愈率 72.5%,总有效率 89.7%.对照组治愈率 60.2%,总有效率 78.9%.两组临床疗效比较,差异具有统计学意义(P<0.05).两组均未出现严重出血事件.结论:微量泵持续输注肝素钠联合尿激酶、华法林治疗下肢深静

  9. The human dimension of fire regimes on Earth

    Science.gov (United States)

    Bowman, David M.J.S.; Balch, Jennifer; Artaxo, Paulo; Bond, William J.; Cochrane, Mark A.; D'Antonio, Carla M.; DeFries, Ruth; Johnston, Fay H.; Keeley, Jon E.; Krawchuk, Meg A.; Kull, Christian A.; Michelle, Mack; Moritz, Max A.; Pyne, Stephen; Roos, Christopher I.; Scott, Andrew C.; Sodhi, Navjot S.; Swetnam, Thomas W.

    2011-01-01

    Humans and their ancestors are unique in being a fire-making species, but 'natural' (i.e. independent of humans) fires have an ancient, geological history on Earth. Natural fires have influenced biological evolution and global biogeochemical cycles, making fire integral to the functioning of some biomes. Globally, debate rages about the impact on ecosystems of prehistoric human-set fires, with views ranging from catastrophic to negligible. Understanding of the diversity of human fire regimes on Earth in the past, present and future remains rudimentary. It remains uncertain how humans have caused a departure from 'natural' background levels that vary with climate change. Available evidence shows that modern humans can increase or decrease background levels of natural fire activity by clearing forests, promoting grazing, dispersing plants, altering ignition patterns and actively suppressing fires, thereby causing substantial ecosystem changes and loss of biodiversity. Some of these contemporary fire regimes cause substantial economic disruptions owing to the destruction of infrastructure, degradation of ecosystem services, loss of life, and smoke-related health effects. These episodic disasters help frame negative public attitudes towards landscape fires, despite the need for burning to sustain some ecosystems. Greenhouse gas-induced warming and changes in the hydrological cycle may increase the occurrence of large, severe fires, with potentially significant feedbacks to the Earth system. Improved understanding of human fire regimes demands: (1) better data on past and current human influences on fire regimes to enable global comparative analyses, (2) a greater understanding of different cultural traditions of landscape burning and their positive and negative social, economic and ecological effects, and (3) more realistic representations of anthropogenic fire in global vegetation and climate change models. We provide an historical framework to promote understanding

  10. The human dimension of fire regimes on Earth.

    Science.gov (United States)

    Bowman, David M J S; Balch, Jennifer; Artaxo, Paulo; Bond, William J; Cochrane, Mark A; D'Antonio, Carla M; Defries, Ruth; Johnston, Fay H; Keeley, Jon E; Krawchuk, Meg A; Kull, Christian A; Mack, Michelle; Moritz, Max A; Pyne, Stephen; Roos, Christopher I; Scott, Andrew C; Sodhi, Navjot S; Swetnam, Thomas W; Whittaker, Robert

    2011-12-01

    Humans and their ancestors are unique in being a fire-making species, but 'natural' (i.e. independent of humans) fires have an ancient, geological history on Earth. Natural fires have influenced biological evolution and global biogeochemical cycles, making fire integral to the functioning of some biomes. Globally, debate rages about the impact on ecosystems of prehistoric human-set fires, with views ranging from catastrophic to negligible. Understanding of the diversity of human fire regimes on Earth in the past, present and future remains rudimentary. It remains uncertain how humans have caused a departure from 'natural' background levels that vary with climate change. Available evidence shows that modern humans can increase or decrease background levels of natural fire activity by clearing forests, promoting grazing, dispersing plants, altering ignition patterns and actively suppressing fires, thereby causing substantial ecosystem changes and loss of biodiversity. Some of these contemporary fire regimes cause substantial economic disruptions owing to the destruction of infrastructure, degradation of ecosystem services, loss of life, and smoke-related health effects. These episodic disasters help frame negative public attitudes towards landscape fires, despite the need for burning to sustain some ecosystems. Greenhouse gas-induced warming and changes in the hydrological cycle may increase the occurrence of large, severe fires, with potentially significant feedbacks to the Earth system. Improved understanding of human fire regimes demands: (1) better data on past and current human influences on fire regimes to enable global comparative analyses, (2) a greater understanding of different cultural traditions of landscape burning and their positive and negative social, economic and ecological effects, and (3) more realistic representations of anthropogenic fire in global vegetation and climate change models. We provide an historical framework to promote understanding

  11. Climate change and future fire regimes: Examples from California

    Science.gov (United States)

    Keeley, Jon E.; Syphard, Alexandra D.

    2016-01-01

    Climate and weather have long been noted as playing key roles in wildfire activity, and global warming is expected to exacerbate fire impacts on natural and urban ecosystems. Predicting future fire regimes requires an understanding of how temperature and precipitation interact to control fire activity. Inevitably this requires historical analyses that relate annual burning to climate variation. Fuel structure plays a critical role in determining which climatic parameters are most influential on fire activity, and here, by focusing on the diversity of ecosystems in California, we illustrate some principles that need to be recognized in predicting future fire regimes. Spatial scale of analysis is important in that large heterogeneous landscapes may not fully capture accurate relationships between climate and fires. Within climatically homogeneous subregions, montane forested landscapes show strong relationships between annual fluctuations in temperature and precipitation with area burned; however, this is strongly seasonal dependent; e.g., winter temperatures have very little or no effect but spring and summer temperatures are critical. Climate models that predict future seasonal temperature changes are needed to improve fire regime projections. Climate does not appear to be a major determinant of fire activity on all landscapes. Lower elevations and lower latitudes show little or no increase in fire activity with hotter and drier conditions. On these landscapes climate is not usually limiting to fires but these vegetation types are ignition-limited. Moreover, because they are closely juxtaposed with human habitations, fire regimes are more strongly controlled by other direct anthropogenic impacts. Predicting future fire regimes is not rocket science; it is far more complicated than that. Climate change is not relevant to some landscapes, but where climate is relevant, the relationship will change due to direct climate effects on vegetation trajectories, as well as

  12. Early warning of atmospheric regime transitions using transfer operators

    Science.gov (United States)

    Tantet, Alexis; Dijkstra, Henk

    2015-04-01

    The existence of persistent midlatitude atmospheric regimes, such as blocking events, with time scales larger than 5-10 days and indications of preferred transition paths between them motivates the development of early-warning indicators of regime transitions. Here, we use a barotropic model of the northern midlatitudes winter flow to study such meta-stable regimes. We look at estimates of transfer operators acting on densities evolving on a reduced phase space spanned by the first Empirical Orthogonal Functions of the streamfunction and develop an early-warning indicator of zonal to blocked flow transition. The study of the spectra of transfer operators estimated for different lags reveals a multi-level structure in the flow as well as the effect of memory on the reduced dynamics due to past interactions between the resolved and unresolved variables. The slowest motions in the reduced phase space are thereby found to have time scales larger than 8 days and to behave as Markovian for larger lags. These motions are associated with meta-stable regimes and their transitions and can be detected as almost-invariant sets of the transfer operator. The early-warning indicator is based on the action on an initial density of products of the transfer operators estimated for sufficiently long lags, making use of the semi-group property of these operators and shows relatively good Peirce skill score. From the energy budget of the model, we are able to explain the meta-stability of the regimes and the existence of preferred transition paths as the manifestation of barotropic instability. Finally, even though the model is highly simplified, the skill of the early warning indicator is promising, suggesting that the transfer operator approach can be used in parallel to an operational deterministic model for stochastic prediction or to assess forecast uncertainty.

  13. How will climate change modify river flow regimes in Europe?

    Directory of Open Access Journals (Sweden)

    C. Schneider

    2012-08-01

    Full Text Available Worldwide, flow regimes are being modified by various anthropogenic impacts and climate change induces an additional risk. Rising evapotranspiration rates, declining snow cover and changing precipitation patterns will interact differently at different locations. Consequently, in distinct climate zones, unequal consequences can be expected in matters of water stress, flood risk, water quality, and food security. In particular, river ecosystems and their vital ecosystem services will be compromised as their species richness and composition have evolved over long time under natural flow conditions. This study aims at evaluating the exclusive impacts of climate change on river flow regimes in Europe. Various flow characteristics are taken into consideration and diverse dynamics are identified for each distinct climate zone in Europe. In order to simulate natural and modified flow regimes, the global hydrology model WaterGAP3 is applied. All calculations for current and future conditions (2050s are carried out on a 5' × 5' European grid. To address uncertainty, climate forcing data of three different global climate models are used to drive WaterGAP3. Finally, the hydrological alterations of different flow characteristics are quantified by the Indicators of Hydrological Alteration approach. Results of our analysis indicate that on European scale, climate change can be expected to modify flow regimes significantly. This is especially the case in the Mediterranean climate zone (due to drier conditions with reduced precipitation across the year and in the continental climate zone (due to reduced snowmelt and drier summers. Regarding single flow characteristics, strongest impacts on timing were found for the boreal climate zone. This applies for both, high and low flows. While low flow magnitudes are likely to be stronger influenced in the Mediterranean climate, high flow magnitudes will be mainly altered in snow climates with warmer summers. At the end

  14. Climate Change and Future Fire Regimes: Examples from California

    Directory of Open Access Journals (Sweden)

    Jon E. Keeley

    2016-08-01

    Full Text Available Climate and weather have long been noted as playing key roles in wildfire activity, and global warming is expected to exacerbate fire impacts on natural and urban ecosystems. Predicting future fire regimes requires an understanding of how temperature and precipitation interact to control fire activity. Inevitably this requires historical analyses that relate annual burning to climate variation. Fuel structure plays a critical role in determining which climatic parameters are most influential on fire activity, and here, by focusing on the diversity of ecosystems in California, we illustrate some principles that need to be recognized in predicting future fire regimes. Spatial scale of analysis is important in that large heterogeneous landscapes may not fully capture accurate relationships between climate and fires. Within climatically homogeneous subregions, montane forested landscapes show strong relationships between annual fluctuations in temperature and precipitation with area burned; however, this is strongly seasonal dependent; e.g., winter temperatures have very little or no effect but spring and summer temperatures are critical. Climate models that predict future seasonal temperature changes are needed to improve fire regime projections. Climate does not appear to be a major determinant of fire activity on all landscapes. Lower elevations and lower latitudes show little or no increase in fire activity with hotter and drier conditions. On these landscapes climate is not usually limiting to fires but these vegetation types are ignition-limited. Moreover, because they are closely juxtaposed with human habitations, fire regimes are more strongly controlled by other direct anthropogenic impacts. Predicting future fire regimes is not rocket science; it is far more complicated than that. Climate change is not relevant to some landscapes, but where climate is relevant, the relationship will change due to direct climate effects on vegetation

  15. Detecting regime shifts in the ocean: Data considerations [review article

    Science.gov (United States)

    deYoung, B.; Harris, R.; Alheit, J.; Beaugrand, G.; Mantua, N.; Shannon, L.

    2004-02-01

    We review observational data sets that have been used to detect regime shifts in the ocean. Through exploration of data time series we develop a definition of a regime shift from a pragmatic perspective, in which a shift is considered as an abrupt change from a quantifiable ecosystem state. We conclude that such changes represent a restructuring of the ecosystem state in some substantial sense that persists for long enough that a new quasi-equilibrium state can be observed. The abruptness of the shift is relative to the life-scale or the reproductive time-scale of the higher predators that are influenced by the shift. In general, the event-forcing is external to the biological ecosystem, usually the physical climate system, but we also identify shifts that can be ascribed to anthropogenic forcing, in our examples fishing. This pragmatic definition allows for several different types of regime shift ranging from simple biogeographic shifts to non-linear state changes. In practice it is quite difficult to determine whether observed changes in an oceanic ecosystem are primarily spatial or temporally regulated. The determination of ecosystem state remains an unresolved, and imprecise, oceanographic problem. We review observations and interpretation from several different oceanic regions as examples to illustrate this pragmatic definition of a regime shift: the Northeast Pacific, the Northwest and Northeast Atlantic, and Eastern Boundary Currents. For each region, different types of data (biological and physical) are available for differing periods of time, and we conclude, with varying degrees of certainty, whether a regime shift is in fact detectable in the data.

  16. Regimes of science production and diffusion: towards a transverse organization of knowledge

    Directory of Open Access Journals (Sweden)

    Anne Marcovich

    2012-01-01

    Full Text Available This article is a contribution to the critical sociology of science perspective introduced and developed by Pierre Bourdieu. The paper proposes a transversalist theory of science and technology production and diffusion. It is here argued that science and technology are comprised of multiple regimes where each regime is historically grounded, possesses its own division of labour, modes of cognitive and artifact production and has specific audiences. The major regimes include the disciplinary regime, utilitarian regime, transitory regime and research-technology regime. Though each regime is autonomous, they are simultaneously closely interlaced. In science and technology, autonomy is not antithetical to interdependence and reciprocity. This study demonstrates for the four specified regimes of production and diffusion that differentiation is not contrary to interaction. In science, differentiation and interaction comprise two sides of the same coin. All regimes exhibit a measure of transversality.

  17. Numerical simulation of flows from free molecular regime to continuum regime by a DVM with streaming and collision processes

    Science.gov (United States)

    Yang, L. M.; Shu, C.; Wu, J.; Wang, Y.

    2016-02-01

    A discrete velocity method (DVM) with streaming and collision processes is presented in this work for simulation of flows from free molecular regime to continuum regime. The present scheme can be considered as a semi-Lagrangian like scheme. At first, we follow the conventional DVM to discretize the phase velocity space by a number of discrete velocities. Then, for each discrete velocity, the kinetic equation with BGK-Shakhov model is integrated in space and time within one time step. As a result, a simple algebraic formulation can be obtained, and its solution can be marched in time by the streaming and collision processes. However, differently from the conventional semi-Lagrangian scheme, the present scheme uses the MUSCL approach with van Albada limiter in the process of reconstructing the distribution function at the surrounding points of the cell center, and the transport distance is controlled in order to avoid extrapolation. This makes the present scheme be capable of simulating the hypersonic rarefied flows. In addition, as compared to the unified gas kinetic scheme (UGKS), the present scheme is simpler and easier for implementation. Thus, the computational efficiency can be improved accordingly. To validate the proposed numerical scheme, test examples from free molecular regime to continuum regime are simulated. Numerical results showed that the present scheme can predict the flow properties accurately even for hypersonic rarefied flows.

  18. Exchange Rate Regimes – A periodical overview and a critical analysis of exchange rate regimes in Kosovo

    Directory of Open Access Journals (Sweden)

    Flamur Bunjaku

    2015-03-01

    Full Text Available Exchange rate regimes and the monetary policy are the key instruments governments use to achieve their economic and financial objectives. Moreover, due to global financial crisis the latter instruments get more importance. Empirical evidences show that exchange rate regimes in Kosovo and its monetary policy throughout their development were mainly influenced by different political and historical developments. In regard of Euroisation of monetary system in Kosovo it was found that this action generated macro - financial stability in terms of inflation and price fluctuation. However, in terms of microeconomic aspects, the unilateral adaptation of Euro as the official currency of Kosovo failed to provide microeconomic advantages such as to export stimulation, and so forth. The main exchange rate regime systems were discussed focusing in their advantages and disadvantages, and it was concluded that there is no commonly accepted theory regarding the optimality of exchange rate regimes. In addition, the global financial crisis impact in the financial system of Kosovo is also discussed and it was found that negative impacts of global financial crisis were moderate and indirect.

  19. 尿激酶不同给药方法治疗动静脉内瘘血栓的疗效观察%Urokinase different medication for the treatment of thecurative effect of venous fistula thrombosis observation

    Institute of Scientific and Technical Information of China (English)

    毛艳华

    2014-01-01

    Objective To observe the urokinase two differentmedication for the treatment of hemodialysisarteriovenous fistula in patients receiving thrombolyticeffect. Methods In our hospital from 2010 October to 2013October autologous artery fistula thrombosis in 26 cases of intravenous, were randomly divided into two groups.Group A:continuous micro pump infusion of thrombolyticgroup;group B:intermittent intravenous thrombolysis group. Results The effect of the two groups were compared with A group of thrombolysis in 11 cases,accounting for 84.6%;group B thrombolysis in 8 cases,accounting for 61.5%. Continuous micro injection pump is better than that of treatment with intermittent intravenousinjection group. The difference between groups was statistically significant at P<0.05. Conclusion Urokinasethrombolysis continued micro pump injection lasting effect,its efficacy and thromboembolism is closely related toearly thrombolytic effect.%目的:观察尿激酶两种不同的给药方法治疗血液透析患者自体动静脉内瘘血栓溶栓的疗效。方法选取我院2010年10月-2013年10月自体动静脉内瘘血栓形成患者26例,随机分为两组。 A组:连续性微泵注射溶栓组;B组:间断静脉注射溶栓组。结果比较两组溶栓的效果A组溶通11例,占84.6%;B组溶通8例,占61.5%。连续性微泵注射溶栓治疗效果优于间断静脉注射组。组间比较差异有统计学意义P<0.05。结论尿激酶持续微泵注射溶栓作用持久,其疗效与血栓栓塞时间密切相关,早期溶栓效果显著。

  20. Mechanical surface treatment of steel-Optimization parameters of regime

    Science.gov (United States)

    Laouar, L.; Hamadache, H.; Saad, S.; Bouchelaghem, A.; Mekhilef, S.

    2009-11-01

    Mechanical treatment process by superficial plastic deformation is employed for finished mechanical part surface. It introduces structural modifications that offer to basic material new properties witch give a high quality of physical and geometrical on superficial layers. This study focuses on the application of burnishing treatment (ball burnishing) on XC48 steel and parameters optimisation of treatment regime. Three important parameters were considered: burnishing force ' Py', burnishing feed 'f' and ball radius 'r'. An empirical model has been developed to illustrate the relationship between these parameters and superficial layer characteristics defined by surface roughness ' Ra' and superficial hardness ' Hv'. A program was developed in order to determine the optimum treatment regimes for each characteristic.

  1. Nano-optomechanical measurement in the photon counting regime

    CERN Document Server

    de Lépinay, Laure Mercier; Rohr, Sven; Gloppe, Arnaud; Kuhn, Aurélien; Verlot, Pierre; Dupont-Ferrier, Eva; Besga, Benjamin; Arcizet, Olivier

    2015-01-01

    Optically measuring in the photon counting regime is a recurrent challenge in modern physics and a guarantee to develop weakly invasive probes. Here we investigate this idea on a hybrid nano-optomechanical system composed of a nanowire hybridized to a single Nitrogen-Vacancy (NV) defect. The vibrations of the nanoresonator grant a spatial degree of freedom to the quantum emitter and the photon emission event can now vary in space and time. We investigate how the nanomotion is encoded on the detected photon statistics and explore their spatio-temporal correlation properties. This allows a quantitative measurement of the vibrations of the nanomechanical oscillator at unprecedentedly low light intensities in the photon counting regime when less than one photon is detected per oscillation period, where standard detectors are dark-noise-limited. These results have implications for probing weakly interacting nanoresonators, for low temperature experiments and for investigating single moving markers.

  2. Pulsar Timing Arrays and Gravity Tests in the Radiative Regime

    CERN Document Server

    Lee, K J

    2014-01-01

    In this paper, we focus on testing gravity theories in the radiative regime using pulsar timing array observations. After reviewing current techniques to measure the dispersion and alternative polarization of gravitational waves, we extend the framework to the most general situations, where the combinations of a massive graviton and alternative polarization modes are considered. The atlas of the Hellings-Downs functions is completed by the new calculations for these dispersive alternative polarization modes. We find that each mode and corresponding graviton mass introduce characteristic features in the Hellings-Downs function. Thus, in principal, we can not only detect each polarization mode, measure the corresponding graviton mass, but also discriminate the different scenarios. In this way, we can test gravity theories in the radiative regime in a generalized fashion, and such method is a direct experiment, where one can address the gauge symmetry of the gravity theories in their linearised limits. Although ...

  3. New Mobilities Regimes in Art and Social Sciences

    DEFF Research Database (Denmark)

    Witzgall, Susanne; Vogl, Gerlinde; Kesselring, Sven

    New Mobilities Regimes analyses how global mobilities are changing the world of today and the role of political and economic power. Bringing together essays by leading scholars and social scientists, including Mimi Sheller and Bülent Diken with the work of well-known artists and art theorists....... Illustrated by a range of studies which examine the regulation and structure of mobility, such as the daily routines of teleworkers, Ukrainian cleaners in Western Europe, the mobility policies of global corporations, and the impact of bicycle policies on public space, New Mobilities Regimes emphasizes...... the routes and crossroads of migration flows as well as at the interaction of mobility and new spatial concepts. The contributors are concerned with both the positive outcomes and the disappointments of the global mobilizations in modern lives. This book is ground-breaking in that it calls...

  4. The case for regime-based water quality standards

    Science.gov (United States)

    Poole, Geoffrey C.; Dunham, J.B.; Keenan, D.M.; Sauter, S.T.; McCullough, D.A.; Mebane, Christopher; Lockwood, Jeffrey C.; Essig, Don A.; Hicks, Mark P.; Sturdevant, Debra J.; Materna, E.J.; Spalding, M.; Risley, John; Deppman, Marianne

    2004-01-01

    Conventional water quality standards have been successful in reducing the concentration of toxic substances in US waters. However, conventional standards are based on simple thresholds and are therefore poorly structured to address human-caused imbalances in dynamic, natural water quality parameters, such as nutrients, sediment, and temperature. A more applicable type of water quality standarda??a a??regime standarda??a??would describe desirable distributions of conditions over space and time within a stream network. By mandating the protection and restoration of the aquatic ecosystem dynamics that are required to support beneficial uses in streams, well-designed regime standards would facilitate more effective strategies for management of natural water quality parameters.

  5. Adaptive two-regime method: application to front propagation

    CERN Document Server

    Robinson, Martin; Erban, Radek

    2013-01-01

    The Adaptive Two-Regime Method (ATRM) is developed for hybrid (multiscale) stochastic simulation of reaction-diffusion problems. It efficiently couples detailed Brownian dynamics simulations with coarser lattice-based models. The ATRM is a generalization of the previously developed Two-Regime Method [Flegg et al, Journal of the Royal Society Interface, 2012] to multiscale problems which require a dynamic selection of regions where detailed Brownian dynamics simulation is used. Typical applications include a front propagation or spatio-temporal oscillations. In this paper, the ATRM is used for an in-depth study of front propagation in a stochastic reaction-diffusion system which has its mean-field model given in terms of the Fisher equation [Fisher, Annals of Eugenics, 1937]. It exhibits a travelling reaction front which is sensitive to stochastic fluctuations at the leading edge of the wavefront. Previous studies into stochastic effects on the Fisher wave propagation speed have focused on lattice-based models...

  6. A Cold-Strontium Laser in the Superradiant Crossover Regime

    CERN Document Server

    Norcia, Matthew A

    2015-01-01

    Recent proposals suggest that lasers based on narrow dipole-forbidden transitions in cold alkaline earth atoms could achieve linewidths that are orders of magnitude smaller than linewidths of any existing lasers. Here, we demonstrate a laser based on the 7.5 kHz linewidth dipole forbidden $^3 $P$_1$ to $^1 $S$_0$ transition in laser-cooled and tightly confined $^{88}$Sr. We can operate this laser in the bad-cavity regime, where coherence is primarily stored in the atoms, or continuously tune to the more conventional good-cavity regime, where coherence is primarily stored in the light field. We show that the cold-atom gain medium can be repumped to achieve quasi steady-state lasing, and demonstrate up to an order of magnitude suppression in the sensitivity of laser frequency to changes in cavity length, the primary limitation for the most frequency stable lasers today.

  7. A Cold-Strontium Laser in the Superradiant Crossover Regime

    Science.gov (United States)

    Norcia, Matthew; Thompson, James

    2016-05-01

    We demonstrate and study a laser based on the 7.5 kHz linewidth dipole forbidden 3 P1 to 1 S0 transition in laser-cooled and tightly confined 88 Sr. We can operate this laser in the bad-cavity or superradiant regime, where coherence is primarily stored in the atoms, or continuously tune to the more conventional good-cavity regime, where coherence is primarily stored in the light field. We show that the cold-atom gain medium can be repumped to achieve quasi steady-state lasing. We also demonstrate up to an order of magnitude suppression in the sensitivity of laser frequency to changes in cavity length, verifying a key feature of proposed narrow linewidth lasers based on dipole-forbidden transitions in alkaline earth atoms.

  8. Option Pricing when the Regime-Switching Risk is Priced

    Institute of Scientific and Technical Information of China (English)

    Tak Kuen Siu; Hailiang Yang

    2009-01-01

    We study the pricing of an option when the price dynamic of the underlying risky asset is governed by a Markov-modulated geometric Brownian motion. We suppose that the drift and volatility of the underlying risky asset are modulated by an observable continuous-time, finite-state Markov chain. We develop a twostage pricing model which can price both the diffusion risk and the regime-switching risk based on the Esscher transform and the minimization of the maximum entropy between an equivalent martingale measure and the real-world probability measure over different states. Numerical experiments are conducted and their results reveal that the impact of pricing regime-switching risk on the option prices is significant.

  9. Onset of the Asymptotic Regime for Finite Orders

    CERN Document Server

    Henson, Joe; Sorkin, Rafael D; Surya, Sumati

    2015-01-01

    We describe a Markov-Chain-Monte-Carlo algorithm which can be used to generate naturally labeled n-element posets at random with a probability distribution of one's choice. Implementing this algorithm for the uniform distribution, we explore the approach to the asymptotic regime in which almost every poset takes on the three-layer structure described by Kleitman and Rothschild (KR). By tracking the n-dependence of several order-invariants, among them the height of the poset, we observe an oscillatory behavior which is very unlike a monotonic approach to the KR regime. Only around n=40 or so does this "finite size dance" appear to give way to a gradual crossover to asymptopia which lasts until n=85, the largest n we have simulated.

  10. Biphoton spectroscopy in a strongly nondegenerate regime of SPDC

    International Nuclear Information System (INIS)

    The absorption spectrum of Cr3+ ions in Al2O3 crystal in the range from 691 nm to 697 nm was measured at the room temperature using the biphoton light generated by nondegenerate spontaneous parametric down-conversion of the He-Cd laser radiation (325 nm) in the LiIO3 crystal. The spectrum of the sample was measured by counting the coincidences when signal photons go through the impurity crystal, whilst the wavelength of the idler photons is resolved by a monochromator. A strongly nondegenerate regime of SPDC, when the difference in frequencies of correlated photons exceeds significantly their spectral width, was used in the biphoton spectroscopy for the first time. Such a regime allows one to obtain an absorption spectrum by measuring the wavelength in a completely different spectral range

  11. Biphoton spectroscopy in a strongly nondegenerate regime of SPDC

    Science.gov (United States)

    Kalachev, A. A.; Kalashnikov, D. A.; Kalinkin, A. A.; Mitrofanova, T. G.; Shkalikov, A. V.; Samartsev, V. V.

    2008-08-01

    The absorption spectrum of Cr3+ ions in Al2O3 crystal in the range from 691 nm to 697 nm was measured at the room temperature using the biphoton light generated by nondegenerate spontaneous parametric down-conversion of the He-Cd laser radiation (325 nm) in the LiIO3 crystal. The spectrum of the sample was measured by counting the coincidences when signal photons go through the impurity crystal, whilst the wavelength of the idler photons is resolved by a monochromator. A strongly nondegenerate regime of SPDC, when the difference in frequencies of correlated photons exceeds significantly their spectral width, was used in the biphoton spectroscopy for the first time. Such a regime allows one to obtain an absorption spectrum by measuring the wavelength in a completely different spectral range.

  12. Adult learning, education, and the labour market inthe employability regime

    Directory of Open Access Journals (Sweden)

    Staffan Nilsson

    2013-10-01

    Full Text Available The purpose of this paper is to draw on the research and scholarly literature to explorethe changing discourses and perspectives concerning adult learning, education, and thelabour market in the employability regime. The focus of the nalysis is a Nordic context.The dominant employability regime maintains a technical-rational perspective onlearning and employability. Education is predominantly regarded as an instrumentalpreparation for the labour market. The future demands of the labour market are largelyunknown, however, and vocational and professional training may not provide sufficientpreparation for the increasing complexities of work. Theoretical discussions have beendominated by an alleged mismatch between individual competence and thequalifications that are required in the world of work. There is no consensus regardinghow the gap should be described, explained, or bridged. New demands on educationaldesign have emerged, and ideas related to liberal education and ‘bildung’ have beenreinserted into the political agenda, offering general preparation for a wider array ofchallenges.

  13. Heavy ion acceleration in the Breakout Afterburner regime

    CERN Document Server

    Petrov, G M; Thomas, A G R; Krushelnick, K; Beg, F N

    2015-01-01

    Theoretical study of heavy ion acceleration from an ultrathin (20 nm) gold foil irradiated by sub-picosecond lasers is presented. Using two dimensional particle-in-cell simulations we identified two highly efficient ion acceleration schemes. By varying the laser pulse duration we observed a transition from Radiation Pressure Acceleration to the Breakout Afterburner regime akin to light ions. The underlying physics and ion acceleration regimes are similar to that of light ions, however, nuances of the acceleration process make the acceleration of heavy ions more challenging. Two laser systems are studied in detail: the Texas Petawatt Laser and the Trident laser, the former having pulse duration 180 fs, intermediate between very short femtosecond pulses and picosecond pulses. Both laser systems generated directional gold ions beams (~10 degrees half-angle) with fluxes in excess of 1011 ion/sr and normalized energy >10 MeV/nucleon.

  14. The regime and the constitutional principles from 1938

    Directory of Open Access Journals (Sweden)

    Florin Grecu

    2012-12-01

    Full Text Available The authoritarian monarchy regime, from 1938-1940, functioned based on an exceptional status, because it was founded on a decree-law of state of siege and therefore all the power in the State was held in the hands of the army colonels and generals. By suspending the political parties, the political pluralism and the Parliament, the basic institutions of a democratic regime were abolished. Press censorship, the denying of the right to manifest and gather were assigned to the army and the militarization of the State institutions became a reality. The proclamation of the King as an absolute leader of the unique party and of the government transformed Romania into a ministerial and undemocratic monarchy, with authoritarian valence.

  15. Automatic Classification of Offshore Wind Regimes With Weather Radar Observations

    DEFF Research Database (Denmark)

    Trombe, Pierre-Julien; Pinson, Pierre; Madsen, Henrik

    2014-01-01

    fluctuations. The information they provide could then be integrated into an advanced prediction system for improving offshore wind power predictability and controllability. In this paper, we address the automatic classification of offshore wind regimes (i.e., wind fluctuations with specific frequency...... systems are extracted from weather radar images. These attributes describe the global intensity, spatial continuity and motion of precipitation echoes on the images. Finally, a CART classification tree is used to find the broad relationships between precipitation attributes and wind regimes......Weather radar observations are called to play an important role in offshore wind energy. In particular, they can enable the monitoring of weather conditions in the vicinity of large-scale offshore wind farms and thereby notify the arrival of precipitation systems associated with severe wind...

  16. THE POLICY AND THE TRADE REGIME IN ROMANIA AFTER 1990

    Directory of Open Access Journals (Sweden)

    Dobrotă Gabriela

    2009-11-01

    Full Text Available The growth of connections between national economies has generated a consolidation of foreign currency activities, requiring a continuous adjusting regime, both for the foreign currency policy and for the exchange rate to the market mechanisms. In Romania, there have been frequent alterations of the foreign currency policy, requiring the need to create a juridical framework adequate for the market economy and the evolution of financial instruments. This paper presents the main characteristics of the trade regime and the exchange rate policy in Romania after 1990. Practically, the paper reflects a radiography of the main events on the Romanian foreign exchange rate within the context of specific conditions of the Romanian economy and in correlation with the measures adopted by countries from the Central Europe.

  17. THE POLICY AND THE TRADE REGIME IN ROMANIA AFTER 1990

    Directory of Open Access Journals (Sweden)

    Dobrotă Gabriela

    2009-11-01

    Full Text Available The growth of connections between national economies has generated a consolidation of foreign currency activities, requiring a continuous adjusting regime, both for the foreign currency policy and for the exchange rate to the market mechanisms. In Romania, there have been frequent alterations of the foreign currency policy, requiring the need to create a juridical framework adequate for the market economy and the evolution of financial instruments. This paper presents the main characteristics of the trade regime and the exchange rate policy in Romania after 1990. Practically, the paper reflects a radiography of the main events on the Romanian foreign exchange rate within the context of specific conditions of the Romanian economy and in correlation with the measures adopted by countries from the Central Europe

  18. Relationship between fire regime and vegetation symphenological timing in Sardinia

    Directory of Open Access Journals (Sweden)

    Carlo Ricotta

    2008-01-01

    Full Text Available Fire is a basic ecological factor that contributes to determine vegetation biodiversity and dynamics in time and space. Fuel characteristics play an essential role in fire ignition and propagation; for instance fuel availability and flammability are closely related to the coarse-scale vegetation phenological trends that directly affect wildfire distribution in time and space. In this perspective, the annual NDVI profiles derived from high temporal resolution satellite images like SPOT Vegetation represent an effective tool for monitoring plant seasonal dynamics at the landscape scale. The objective of this study consists in relating the wildfire regime in Sardinia during 2000-2004 with the remotely sensed phenological parameters of the vegetation, segmented according to potential natural vegetation characteristics. The results of our study highlight a good correspondence between the NDVI temporal dynamics of the potential natural vegetation of Sardinia and the corresponding fires regime characteristics.

  19. $H_{2}$ Formation on Interstellar Grains in Different Physical Regimes

    CERN Document Server

    Biham, O; Katz, N; Pirronello, V; Vidali, G

    1998-01-01

    An analysis of the kinetics of H2 formation on interstellar dust grains is presented using rate equations. It is shown that semi-empirical expressions that appeared in the literature represent two different physical regimes. In particular, it is shown that the expression given by Hollenbach, Werner and Salpeter [ApJ, 163, 165 (1971)] applies when high flux, or high mobility, of H atoms on the surface of a grain, makes it very unlikely that H atoms evaporate before they meet each other and recombine. The expression of Pirronello et al.\\ [ApJ, 483, L131 (1997)] -- deduced on the basis of accurate measurements on realistic dust analogue -- applies to the opposite regime (low coverage and low mobility). The implications of this analysis for the understanding of the processes dominating in the Interstellar Medium are discussed.

  20. Accretion to a Magnetized Neutron Star in the "Propeller" Regime

    CERN Document Server

    Toropina, O D; Lovelace, R V E

    2006-01-01

    We investigate spherical accretion to a rotating magnetized star in the "propeller" regime using axisymmetric resistive magnetohydrodynamic simulations. The regime is predicted to occur if the magnetospheric radius is larger than the corotation radius and smaller than the light cylinder radius. The simulations show that accreting matter is expelled from the equatorial region of the magnetosphere and that it moves away from the star in a supersonic, disk-shaped outflow. At larger radial distances the outflow slows down and becomes subsonic. The equatorial matter outflow is initially driven by the centrifugal force, but at larger distances the pressure gradient force becomes significant. We find the fraction of the Bondi accretion rate which accretes to the surface of the star.