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Sample records for 01lla 01llb k58

  1. Preparation of a Lipopolysaccharide from Escherichia coli 01lla, 01llb, k58: h21 bacterial wall, labeled with carbon-14; Preparacion de un lipopolisacarido de la pared baceteriana de escherichia coli 01lla, 01llb, K58: H21, marcado con carbono-14

    Energy Technology Data Exchange (ETDEWEB)

    Solano Aunon, M. L.; Pacheco Lopez, J.; Garcia Pineda, M. D.; Roca, M.; Bayon, A.

    1981-07-01

    A brief description of the morphological and chemical structure of Li po polysaccharides is given, as well as its occurrence in nature and its mechanisms of action. It is emphasized the usefulness for actual biochemical and biomedical research of the labeled Lipopolysaccharide. The method for the labelling, isolation and purification of 14''C-Lipopolysacchari de is described. (Author) 23 refs.

  2. Glycosaminoglycans enhance the fibrillation propensity of the ß2-microglobulin cleavage variant--¿K58-ß2m

    DEFF Research Database (Denmark)

    Corlin, Dorthe B; Johnsen, Christina K; Nissen, Mogens H

    2010-01-01

    in the early aggregates, hereby promoting the assembly of these into fibrils, whereas the copper ions appear to have a destabilizing effect on the monomers. This keeps them in a structure forming amorphous aggregates for a longer period of time, leading to the formation of spherical bodies followed....... In this study, we show that fibrillogenesis of a cleavage variant of ß2m, ¿K58-ß2m, which can be found in the circulation of hemodialysis patients and is able to fibrillate at near-physiological pH in vitro, is affected by the presence of copper ions and heparan sulfate. It is found that the fibrils generated...... when heparan sulfate is present have increased length and diameter, and possess enhanced stability and seeding properties. However, when copper ions are present the fibrils are short, thin and less stable, and form at a slower rate. We suggest that heparan sulfate stabilizes the cleaved monomers...

  3. Observation of a 331 K (58 degrees C) spin transition for bis[hydrotris (1,2,4-triazol-1-yl)borate]iron(II) by variable temperature infrared spectroscopy and magnetic susceptibility measurements

    NARCIS (Netherlands)

    van Koningsbruggen, Petra J.; Miller, Joel S.

    2008-01-01

    Fe{HB(C(2)H(2)N(3))(3)}(2) is observed by variable temperature infrared and magnetic studies to have a spin transition between the low spin S = 0 and high spin S = 2 states at 331 K (58 degrees C) with thermal hysteresis of similar to 1.5 K. Changes in the triazole ligand IR absorptions demonstrate

  4. EVALUATION OF MANDIBULAR HARD AND SOFT TISSUES IN CLEFT PATIENTS*

    Directory of Open Access Journals (Sweden)

    Işıl ARAS

    2017-04-01

    Full Text Available Purpose: The aim of this study was to compare the mandibular hard and soft tissue measurements of unilateral and bilateral cleft lip and palate patients with non-cleft individuals. Materials and Methods: The study sample comprised of lateral cephalograms of 45 subjects. Sample included 15 non-cleft (NC, 15 unilateral cleft lip and palate (UCLP and 15 bilateral cleft lip and palate (BCLP cases whose age were between 15 to 17. 1 angular 13 linear measurements were carried out using Arnett and Gunson soft tissue cephalometric analysis and 4 angular measurements were calculated with Steiner Analysis. Results: Mandibular incisor inclinations relative to the occlusal plane (Md1-Md OP were significantly greater and mandibular incisor projections (Md1-TVL were significantly retrusive in cleft subjects (p<0.05. Projection values pertaining to lower lip anterior (LLA-TVL, soft tissue B point (B’-TVL, and soft tissue pogonion (Pog’-TVL were significantly deficient as well in cleft patients (p<0.05. Sagittal position of the maxilla (SNA (p<0.001 and intermaxillary relation of the jaws (ANB were significantly deficient in UCLP subjects (p<0.05 and BCLP individuals (p<0.01. LLA-TVL and B’-TVL correlated with SNB in cleft patients (p<0.05. Conclusion: Decreased lower lip and chin projection values suggest that mandibular region of cleft patients should be taken into account in forming the treatment plan to improve the esthetic outcome of orthodontic and plastic surgery interventions.

  5. Transformational leadership sub-dimensions and their link to leaders' personality and performance

    NARCIS (Netherlands)

    Deinert, A.; Homan, A.C.; Boer, D.; Voelpel, S.C.; Gutermann, D.

    2015-01-01

    The multi-dimensionality of the transformational leadership construct has been under debate in the last decades. To shed more light on this issue, we conducted a meta-analysis (k = 58 studies), examining the transformational leadership sub-dimensions and their links to leader personality and perform

  6. Transformational leadership sub-dimensions and their link to leaders' personality and performance

    NARCIS (Netherlands)

    Deinert, A.; Homan, A.C.; Boer, D.; Voelpel, S.C.; Gutermann, D.

    2015-01-01

    The multi-dimensionality of the transformational leadership construct has been under debate in the last decades. To shed more light on this issue, we conducted a meta-analysis (k = 58 studies), examining the transformational leadership sub-dimensions and their links to leader personality and

  7. Studies on the Biology of the Tube-Building Amphipod Microdeutopus gryllotalpa. A Model for Marine Fouling Processes.

    Science.gov (United States)

    1984-01-01

    144-153. Dingle, H. & Caldwell, R.L. 1972. Reproductive and maternal behaicr of the mantis shrimp Gonodactylus bredini manning (Crustacea: Stomatopoda...restricted to Acknowledgements. I thank P. Fenimore for his help in the peracarids. Individuals of the snapping shrimp Alpheus collecting animals, and R...Knowlton. N.: Sexual selection and dimorphism in two demes of mar. biol. Ass. U.K. 58. 585-596 (1978) a symbiotic. snapping shrimp . Evolution 34. 161

  8. Half-a-Century of the »Körmend Growth Study«

    OpenAIRE

    Eiben, O. G.; Tóth, G.

    2000-01-01

    The authors give a sketch about the „Körmend Growth Study« which is series of cross-sectional growth studies, carried out in Körmend, a small town in Western Hungary. The first investigation was carried out in 1958 (K-58) and it has been repeated every ten years (K-68, K-78, K-88, and K-98). All 3-18 year-old healthy boys and girls in nurseries and schools in Körmend were involved in the study. Twenty-three body measurements were taken. This paper focuses on changes in height, ...

  9. Amino Acid Substitutions That Affect Receptor Binding and Stability of the Hemagglutinin of Influenza A/H7N9 Virus

    Science.gov (United States)

    Schrauwen, Eefje J. A.; Burke, David F.; Rimmelzwaan, Guus F.; Herfst, Sander; Fouchier, Ron A. M.

    2016-01-01

    Receptor-binding preference and stability of hemagglutinin have been implicated as crucial determinants of airborne transmission of influenza viruses. Here, amino acid substitutions previously identified to affect these traits were tested in the context of an A/H7N9 virus. Some combinations of substitutions, most notably G219S and K58I, resulted in relatively high affinity for α2,6-linked sialic acid receptor and acid and temperature stability. Thus, the hemagglutinin of the A/H7N9 virus may adopt traits associated with airborne transmission. PMID:26792744

  10. Unfolding, aggregation, and seeded amyloid formation of lysine-58-cleaved beta(2)-microglobulin

    DEFF Research Database (Denmark)

    Heegaard, N.H.H.; Jørgensen, T.J.D.; Rozlosnik, N.;

    2005-01-01

    beta(2)-Microglobulin (beta(2)m) is the amyloidogenic protein in dialysis-related amyloidosis, but the mechanisms underlying beta(2)m fibrillogenesis in vivo are largely unknown. We study a structural variant of beta(2)M that has been linked to cancer and inflammation and may be present in the ci......beta(2)-Microglobulin (beta(2)m) is the amyloidogenic protein in dialysis-related amyloidosis, but the mechanisms underlying beta(2)m fibrillogenesis in vivo are largely unknown. We study a structural variant of beta(2)M that has been linked to cancer and inflammation and may be present...... in the circulation of dialysis patients. This beta(2)M variant, Delta K58-beta(2)m, is a disulfide-linked two-chain molecule consisting of amino acid residues 1-57 and 59-99 of intact beta(2)m, and we here demonstrate and characterize its decreased conformational stability as compared to wild-type (wt) beta(2)M......, and at 37 degrees C the half-time for unfolding is more than 170-fold faster than at 15 degrees C. Conformational changes are also reflected by a very prominent Congo red binding of Delta K58-beta(2)m at 37 degrees C, by the evolution of thioflavin T fluorescence, and by changes in intrinsic fluorescence...

  11. Influence of acid-soluble proteins from bivalve Siliqua radiata ligaments on calcium carbonate crystal growth

    Science.gov (United States)

    Huang, Zeng-Qiong; Zhang, Gang-Sheng

    2016-08-01

    In vitro biomimetic synthesis of calcium carbonate (CaCO3) in the presence of shell proteins is a heavily researched topic in biomineralization. However, little is known regarding the function of bivalve ligament proteins in the growth of CaCO3 crystals. In this study, using fibrous protein K58 from Siliqua radiata ligaments or coverslips as substrates, we report the results of our study of CaCO3 precipitation in the presence or absence of acid-soluble proteins (ASP) from inner ligament layers. ASP can disturb the controlling function of K58 or a coverslip on the crystalline phase, resulting in the formation of aragonite, calcite, and vaterite. In addition, we identified the following four primary components from ASP by mass spectroscopy: alkaline phosphatase (ALP), ABC transporter, keratin type II cytoskeletal 1 (KRT 1), and phosphate ABC transporter, phosphate-binding protein (PstS). Further analysis revealed that the first three proteins and especially ALP, which is important in bone mineralisation, could affect the polymorphism and morphology of CaCO3 crystals by trapping calcium ions in their domains. Our results indicate that ALP may play an important role in the formation of aragonite in S. radiata ligaments. This paper may facilitate our understanding of the biomineralization process.

  12. Sumoylation Inhibits the Growth Suppressive Properties of Ikaros.

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    Apostol Apostolov

    Full Text Available The Ikaros transcription factor is a tumor suppressor that is also important for lymphocyte development. How post-translational modifications influence Ikaros function remains partially understood. We show that Ikaros undergoes sumoylation in developing T cells that correspond to mono-, bi- or poly-sumoylation by SUMO1 and/or SUMO2/3 on three lysine residues (K58, K240 and K425. Sumoylation occurs in the nucleus and requires DNA binding by Ikaros. Sumoylated Ikaros is less effective than unsumoylated forms at inhibiting the expansion of murine leukemic cells, and Ikaros sumoylation is abundant in human B-cell acute lymphoblastic leukemic cells, but not in healthy peripheral blood leukocytes. Our results suggest that sumoylation may be important in modulating the tumor suppressor function of Ikaros.

  13. Identification of amino acid residues involved in the interaction between measles virus Haemagglutin (MVH) and its human cell receptor (signaling lymphocyte activation molecule, SLAM).

    Science.gov (United States)

    Xu, Qin; Zhang, Peng; Hu, Chunling; Liu, Xin; Qi, Yipeng; Liu, Yingle

    2006-07-31

    Signaling lymphocyte activation molecule (SLAM; also known as CD150) is a newly identified cellular receptor for measles virus (MV). The interaction between MV Haemagglutin (MVH) and SLAM is an initial step for MV entry. We have identified several novel SLAM binding sites at residues S429, T436 and H437 of MVH protein and MVH mutants in these residues dramatically decrease the ability to interaction with the cell surface SLAM and fail to coprecipitation with SLAM in vivo as well as malfunction in syncytium formation. At the same time, K58, S59 and H61 of SLAM was also identified to be critical for MVH and SLAM binding. Further, these residues may be useful targets for the development of measles therapy.

  14. Angular momentum and topological dependence of Kepler's Third Law in the Broucke-Hadjidemetriou-H\\'enon family of periodic three-body orbits

    CERN Document Server

    Janković, Marija R

    2016-01-01

    We use 57 recently found topological satellites of Broucke-Hadjidemetriou-Henon's periodic orbits with values of the topological exponent $k$ ranging from $k$ = 3 to $k$ = 58 to plot the angular momentum $L$ as a function of the period $T$, with both $L$ and $T$ rescaled to energy $E=-\\frac12$. Upon plotting $L(T/k)$ we find that all our solutions fall on a curve that is virtually indiscernible by naked eye from the $L(T)$ curve for non-satellite solutions. The standard deviation of the satellite data from the sixth-order polynomial fit to the progenitor data is $\\sigma = 0.13$. This regularity supports Henon's 1976 conjecture that the linearly stable Broucke-Hadjidemetriou-Henon orbits are also perpetually, or Kolmogorov-Arnold-Moser stable.

  15. Hydrogenation/Dehydrogenation Performances of the MgH2-WS2 Composites

    Institute of Scientific and Technical Information of China (English)

    WANG Jiasheng; ZHANG Wei; CHENG Ying; KE Dandan; HAN Shumin

    2015-01-01

    The hydrogenation/dehydrogenation kinetics and thermodynamic behaviors of the MgH2-WS2 composites were investigated. The TPD (Temperature-Programmed-Desorption) curves showed that the onset dehydrogenation temperature of the MgH2 + 20wt% WS2 composite was 615 K, 58 K lower than that of the pristine MgH2. The kinetic measurements showed that within 21 min, the MgH2 + 20wt% WS2 composite could absorb 2.818wt% at 423 K, and release 4.244 wt% of hydrogen at 623 K, while the hydriding/dehydriding capacity of MgH2 reached only 0.979wt% and 2.319wt% respectively under identical conditions. The improvement of hydrogenation/dehydrogenation performances for the composite was attributed to the co-catalytic effect between the new phases W and MgS which formed during the ball-milling process.

  16. Nutritional disorder of lettuce cv. Veronica in nutrient solution with suppressed macronutrients

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    Thiago Batista Firmato de Almeida

    2011-06-01

    Full Text Available Lettuce is the most popular of the leafy vegetables. It is known worldwide and its consumption occurs mainly in the natural form. The objective was to evaluate the effect of macronutrient omission on the growth and nutritional status of the lettuce cv. Veronica, and to describe the visual symptoms of nutritional deficiency. The treatments complete consisted of the solution and the individual omission of N, P, K, Ca, Mg and S, under a completely randomized design with three replications. Plants were grown in Hoagland & Arnon, in pots (8L. At 56 days after transplant we evaluated plant height, leaf number, leaf area, SPAD index, dry matter of shoots, roots and whole plant, levels of macronutrients in shoots and roots, and the nature of nutritional disorders. The omission of nutrients affected the growth variables. The nutrients found in lettuce plants from nutrient solution and the complete omission in the shoot were, respectively, N= 23.2 to 9.5, P= 5.4 to 1.3, K= 58,9 to 3.2, Ca= 12.1 to 3.6, Mg= 5.5 to 0.7 and S= 3.2 to 1.5g.kg-1. The omission of macronutrients caused quality losses, since it affected the nutrition of the vegetables and this resulted in morphological changes, reflected as symptoms of deficiency for each nutrient.

  17. 2010年福建南平及永安监测点致泻大肠杆菌的监测报告%Surveillance of diarrheagenic Escherichia coli infection in Nanping and Yongan monitoring sites of Fujian in 2010

    Institute of Scientific and Technical Information of China (English)

    林杰; 陈爱平; 陈建辉; 李玉燕; 杨劲松; 郑金凤

    2011-01-01

    目的:为了解福建省腹泻病人中致泻大肠杆菌的感染情况.方法:应用多重PCR或单重PCR方法检测致泻大肠杆菌的EPEC/EHEC eaeA基因、EHEC stx基因、EAEC aggR基因、EIECipaH、EIEC virA、ETEC ST、ETEC LT、EPECbp基因.API 20E生化鉴定条进行生化试验.血清学鉴定.结果:2010年度共分离得19株致泻大肠杆菌,检出率为10.9%.1株EAEC;1株tEPEC;4株aEPEC; 13株ETEC.19株分离的致泻大肠杆菌经API 20E鉴定均为大肠埃希菌.1株tEPEC与EPEC三种多价诊断血清型均不凝集,而1株aEPEC血清型为O111:K58(B4).13株ETEC菌株血清型:多数为O6:K15,1株O25:K19(L),3株未能分型.结论:监测结果对于我们了解福建省致泻大肠杆菌感染情况,预警潜在发生疫情的可能性有其重要性.这些菌种的获得可以为下一步研究其毒力特征、分子分型及耐药性积累原始材料.%Objective:To understand the condition of Diarrheagenic Escherichia coli (DEC) infection in Fujian province. Methods: Genes such as EPEC/EHEC eaek, EHEC stx, EAEC aggR, EIEC ipaH, EIEC virA, ETEC ST, ETEC LT, EPEC bfp were detected by multiplex or single PCR. Biochemical identification was determined by API 20E strip and serotyping was performed. Results: 19 strains of DEC (1 EAEC, 1 tEPEC, 4 aEPEC and 13 ETEC) were isolated in the surveillance in 2010, and the detection rate was 10.9%. All isolated strains were identified as E. Coli by API 20E strip. 1 tEPEC didn't agglutinate with EPEC multivalence diagnostic serum, whereas 1 aEPEC agglutinated with EPEC serum 0111 :K58( B4). Serotypes of 13 ETEC were mainly 06:K15, while the others were 1 strain for O25:K19(L) , 3 strains untyped. Conclusion: The surveillance is important to understand DEC infection in Fujian province and early warn the potential of DEC epidemic. The isolated strains provide material for further study of their virulence characteristics, molecular typing and antibiotic resistance.

  18. Molecular and Genetic Characterization of HIV-1 Tat Exon-1 Gene from Cameroon Shows Conserved Tat HLA-Binding Epitopes: Functional Implications

    Science.gov (United States)

    Teto, Georges; Fonsah, Julius Y.; Tagny, Claude T.; Mbanya, Dora; Nchindap, Emilienne; Kenmogne, Leopoldine; Fokam, Joseph; Njamnshi, Dora M.; Kouanfack, Charles; Njamnshi, Alfred K.; Kanmogne, Georgette D.

    2016-01-01

    HIV-1 Tat plays a critical role in viral transactivation. Subtype-B Tat has potential use as a therapeutic vaccine. However, viral genetic diversity and population genetics would significantly impact the efficacy of such a vaccine. Over 70% of the 37-million HIV-infected individuals are in sub-Saharan Africa (SSA) and harbor non-subtype-B HIV-1. Using specimens from 100 HIV-infected Cameroonians, we analyzed the sequences of HIV-1 Tat exon-1, its functional domains, post-translational modifications (PTMs), and human leukocyte antigens (HLA)-binding epitopes. Molecular phylogeny revealed a high genetic diversity with nine subtypes, CRF22_01A1/CRF01_AE, and negative selection in all subtypes. Amino acid mutations in Tat functional domains included N24K (44%), N29K (58%), and N40K (30%) in CRF02_AG, and N24K in all G subtypes. Motifs and phosphorylation analyses showed conserved amidation, N-myristoylation, casein kinase-2 (CK2), serine and threonine phosphorylation sites. Analysis of HLA allelic frequencies showed that epitopes for HLAs A*0205, B*5301, Cw*0401, Cw*0602, and Cw*0702 were conserved in 58%–100% of samples, with B*5301 epitopes having binding affinity scores > 100 in all subtypes. This is the first report of N-myristoylation, amidation, and CK2 sites in Tat; these PTMs and mutations could affect Tat function. HLA epitopes identified could be useful for designing Tat-based vaccines for highly diverse HIV-1 populations, as in SSA. PMID:27438849

  19. Budgeting of major nutrients and the mitigation options for nutrient mining in semi-arid tropical agro-ecosystem of Tamil Nadu, India using NUTMON model.

    Science.gov (United States)

    Surendran, U; Rama Subramoniam, S; Raja, P; Kumar, V; Murugappan, V

    2016-04-01

    Mining of nutrients from soil is a major problem in developing countries causing soil degradation and threaten long-term food production. The present study attempts to apply NUTrient MONitoring (NUTMON) model for carrying out nutrient budgeting to assess the stocks and flows of nitrogen (N), phosphorus (P), and potassium (K) in defined geographical unit based on the inputs, viz., mineral fertilizers, manures, atmospheric deposition, and sedimentation, and outputs, viz., harvested crop produces, residues, leaching, denitrification, and erosion losses. The study area covers Coimbatore and Erode Districts, which are potential agricultural areas in western agro-ecological zone of Tamil Nadu, India. The calculated nutrient balances for both the districts at district scale, using NUTMON methodology, were negative for nitrogen (N -3.3 and -10.1 kg ha(-1)) and potassium (K -58.6 and -9.8 kg ha(-1)) and positive for phosphorus (P +14.5 and 20.5 kg ha(-1)). Soil nutrient pool has to adjust the negative balance of N and K; there will be an expected mining of nutrient from the soil reserve. A strategy was attempted for deriving the fertilizer recommendation using Decision Support System for Integrated Fertilizer Recommendation (DSSIFER) to offset the mining in selected farms. The results showed that when DSSIFER recommended fertilizers are applied to crops, the nutrient balance was positive. NUTMON-Toolbox with DSSIFER would serve the purpose on enhancing soil fertility, productivity, and sustainability. The management options to mitigate nutrient mining with an integrated system approach are also discussed.

  20. A shock tube and theoretical study on the pyrolysis of 1,4-dioxane.

    Science.gov (United States)

    Yang, X; Jasper, A W; Giri, B R; Kiefer, J H; Tranter, R S

    2011-03-07

    The dissociation of 1, 2 and 4% 1,4-dioxane dilute in krypton was studied in a shock tube using laser schlieren densitometry, LS, for 1550-2100 K with 56 ± 4 and 123 ± 3 Torr. Products were identified by time-of-flight mass spectrometry, TOF-MS. 1,4-dioxane was found to initially dissociate via C-O bond fission followed by nearly equal contributions from pathways involving 2,6 H-atom transfers to either the O or C atom at the scission site. The 'linear' species thus formed (ethylene glycol vinyl ether and 2-ethoxyacetaldehyde) then dissociate by central fission at rates too fast to resolve. The radicals produced in this fission break down further to generate H, CH(3) and OH, driving a chain decomposition and subsequent exothermic recombination. High-level ab initio calculations were used to develop a potential energy surface for the dissociation. These results were incorporated into an 83 reaction mechanism used to simulate the LS profiles with excellent agreement. Simulations of the TOF-MS experiments were also performed with good agreement for consumption of 1,4-dioxane. Rate coefficients for the overall initial dissociation yielded k(123Torr) = (1.58 ± 0.50) × 10(59) × T(-13.63) × exp(-43970/T) s(-1) and k(58Torr) = (3.16 ± 1.10) × 10(79) × T(-19.13) × exp(-51326/T) s(-1) for 1600 < T < 2100 K.

  1. Molten salt CO2 capture and electro-transformation (MSCC-ET) into capacitive carbon at medium temperature: effect of the electrolyte composition.

    Science.gov (United States)

    Deng, Bowen; Chen, Zhigang; Gao, Muxing; Song, Yuqiao; Zheng, Kaiyuan; Tang, Juanjuan; Xiao, Wei; Mao, Xuhui; Wang, Dihua

    2016-08-15

    Electrochemical transformation of CO2 into functional materials or fuels (i.e., carbon, CO) in high temperature molten salts has been demonstrated as a promising way of carbon capture, utilisation and storage (CCUS) in recent years. In a view of continuous operation, the electrolysis process should match very well with the CO2 absorption kinetics. At the same time, in consideration of the energy efficiency, a molten salt electrochemical cell running at lower temperature is more beneficial to a process powered by the fluctuating renewable electricity from solar/wind farms. Ternary carbonates (Li : Na : K = 43.5 : 31.5 : 25.0) and binary chlorides (Li : K = 58.5 : 41.5), two typical kinds of eutectic melt with low melting points and a wide electrochemical potential window, could be the ideal supporting electrolyte for the molten salt CO2 capture and electro-transformation (MSCC-ET) process. In this work, the CO2 absorption behaviour in Li2O/CaO containing carbonates and chlorides were investigated on a home-made gas absorption testing system. The electrode processes as well as the morphology and properties of carbon obtained in different salts are compared to each other. It was found that the composition of molten salts significantly affects the absorption of CO2, electrode processes and performance of the product. Furthermore, the relationship between the absorption and electro-transformation kinetics are discussed based on the findings.

  2. Development of The Viking Speech Scale to classify the speech of children with cerebral palsy.

    Science.gov (United States)

    Pennington, Lindsay; Virella, Daniel; Mjøen, Tone; da Graça Andrada, Maria; Murray, Janice; Colver, Allan; Himmelmann, Kate; Rackauskaite, Gija; Greitane, Andra; Prasauskiene, Audrone; Andersen, Guro; de la Cruz, Javier

    2013-10-01

    Surveillance registers monitor the prevalence of cerebral palsy and the severity of resulting impairments across time and place. The motor disorders of cerebral palsy can affect children's speech production and limit their intelligibility. We describe the development of a scale to classify children's speech performance for use in cerebral palsy surveillance registers, and its reliability across raters and across time. Speech and language therapists, other healthcare professionals and parents classified the speech of 139 children with cerebral palsy (85 boys, 54 girls; mean age 6.03 years, SD 1.09) from observation and previous knowledge of the children. Another group of health professionals rated children's speech from information in their medical notes. With the exception of parents, raters reclassified children's speech at least four weeks after their initial classification. Raters were asked to rate how easy the scale was to use and how well the scale described the child's speech production using Likert scales. Inter-rater reliability was moderate to substantial (k>.58 for all comparisons). Test-retest reliability was substantial to almost perfect for all groups (k>.68). Over 74% of raters found the scale easy or very easy to use; 66% of parents and over 70% of health care professionals judged the scale to describe children's speech well or very well. We conclude that the Viking Speech Scale is a reliable tool to describe the speech performance of children with cerebral palsy, which can be applied through direct observation of children or through case note review. Copyright © 2013 Elsevier Ltd. All rights reserved.

  3. Position for Site-Specific Attachment of a DOTA Chelator to Synthetic Affibody Molecules Has a Different Influence on the Targeting Properties of 68Ga-Compared to 111In-Labeled Conjugates

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    Hadis Honarvar

    2014-12-01

    Full Text Available Affibody molecules, small (7 kDa scaffold proteins, are a promising class of probes for radionuclide molecular imaging. Radiolabeling of Affibody molecules with the positron-emitting nuclide 68Ga would permit the use of positron emission tomography (PET, providing better resolution, sensitivity, and quantification accuracy than single-photon emission computed tomography (SPECT. The synthetic anti-HER2 ZHER2:S1 Affibody molecule was conjugated with DOTA at the N-terminus, in the middle of helix 3, or at the C-terminus. The biodistribution of 68Ga- and 111In-labeled Affibody molecules was directly compared in NMRI nu/nu mice bearing SKOV3 xenografts. The position of the chelator strongly influenced the biodistribution of the tracers, and the influence was more pronounced for 68Ga-labeled Affibody molecules than for the 111In-labeled counterparts. The best 68Ga-labeled variant was 68Ga-[DOTA-A1]-ZHER2:S1 which provided a tumor uptake of 13 ± 1 %ID/g and a tumor to blood ratio of 39 ± 12 at 2 hours after injection. 111In-[DOTA-A1]-ZHER2:S1 and 111In-[DOTA-K58]-ZHER2:S1 were equally good at this time point, providing a tumor uptake of 15 to 16 %ID/g and a tumor to blood ratio in the range of 60 to 80. In conclusion, the selection of the best position for a chelator in Affibody molecules can be used for optimization of their imaging properties. This may be important for the development of Affibody-based and other protein-based imaging probes.

  4. Electrocardiography and echocardiography in athletic heart imagining

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    D Biały

    2003-03-01

    Full Text Available The aim of the study was evaluation of electrocardiographic and echocardiographic parameters in athletes. „Athletic heart” characteristics were compared with fit persons’ heart. 96 athletes participated in the study. Sportsmen were divided into: static (S, dynamic (D and composite (SD exercise groups and Polish (I, European (II, World (III champions. 30 students from Sport Academy formed the control group (K. Electrocardiographic and echocardiographic examination were performed in everyone. As regards the type of exercise, the end-systolic left ventricular (LV dimension was smaller in S in comparison with D and SD (28.9 vs 32.2 and 32.68 mm; P<0.05. LV mass was bigger in D in comparison with K (273.2 vs 218.6 g; P<0.05. Medium Pulmonary Artery Pressure (MPAP in S and SD was lower in respect to D and K (11.75; 11.08 vs 15.52; 17.43 mm Hg; P<0.05. We observed lower heart rate in D, SD in comparison with K (58.64; 60.54 vs 68.8; P<0.05, bigger R wave amplitude in V5 (RV5 (21.65; 23.5 vs 15.03 mm; P<0.05 and V6 (RV6 (23.5 vs 15.3 mm; P<0.05 in group S in respect to K. LV mass was bigger in III than in K (261.3 vs 218.6 g; P<0.05. MPAP was lower in I and II in comparison with K (11.42; 13.13 vs 17.43 mmHg; P<0.05. HR was lower in categories I, II than in K (61.32; 60.13 vs 68.8; P<0.05, RV5 was bigger in I in comparison with K (19.5 vs 15.03 mm; P<0.05. The electrocardiography and echocardiography proves to find some significant differences between athletic and fit persons’ heart especially as concerns MPAP, RV5, RV6 values.

  5. Export of soil macronutrients in area cultivated with maize to feeding feedlot steers

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    Robson Kyoshi Ueno

    2013-12-01

    Full Text Available The maize cultivated for forage requires special care with soil management. The nutrients exportation by silage might cause reducing of soil fertility, with consequent decrease of yield and nutritional quality of posterior crops. The aim of this work was assess levels of macronutrients in maize plant fragments (stem, leaves, bracts plus cob and grains, at stage of ensilage, in entire plant (silage forage and in the mature grains; measure the nutrients exportation from soil by forage or grains. After, two diets were used in feedlot cattle, first was based on silage and other based in grains, to appraise the potential of nutrients to return into soil by manure and the final account of nutrients balance. The levels of N, P, K, Ca and Mg from forage were, respectively: 1,37; 0,24; 0,81; 0,2 and 0,22%; and in grains: 1,8; 0,28; 0,34; 0,12; and 0,11%. The fertilization performed was enough to supply extracted nutrients when was harvested only grains, promoting deficits in soil of 93 kg ha-1 of N and 84 kg ha-1 of K when was harvested forage. The manure of animals feed with silage+concentrated, compared to diet without roughage (80% grains and 20% protein core, cycled different amounts of N (276,28 vs 59,37, P (128,12 vs 33,37, K (58,32 vs 5,38, Ca (177,7 vs 78,89 and Mg (43,64 vs 11,81 in the cultivated area. The evaluated production systems presented capacity to maintain the levels of nutrients at soil through replacement via manure, and might contribute to the enrichment of fertility and reduce costs with mineral fertilizers in successive crops. The system which forage was used presented deficit of 25 kg ha-1 de K, probably due to insufficient amount of K applied, which recommendation is frequently made to grain production.

  6. Quasi-2D characteristics in the ac susceptibility response of polycrystalline (Bi,Pb)SrCaCuO/2223 multiphase systems

    Energy Technology Data Exchange (ETDEWEB)

    Mihalache, V. [Department of Low Temperature Physics and Superconductivity, National Institute for Materials Physics, POB MG-7, 077125 Bucharest-Magurele (Romania)], E-mail: vmihal@infim.ro; Cosereanu, L. [METRA, P.O. Box 51-16, 076550 Bucharest (Romania); Miu, L. [Department of Low Temperature Physics and Superconductivity, National Institute for Materials Physics, POB MG-7, 077125 Bucharest-Magurele (Romania)

    2008-01-01

    Fundamental and third harmonic ac susceptibility measurements as a function of temperature T, ac magnetic field, and dc magnetic field have been performed on Bi(Pb)SrCaCuO/2223 bulk samples consisting of both Bi{sub 2}Sr{sub 2}Ca{sub 2}Cu{sub 3}O{sub 10+{delta}} (2223) and Bi{sub 2}Sr{sub 2}CaCu{sub 2}O{sub 8+{delta}} (2212) phases. Since the {chi}{sub 1}{sup ''}(T) peak corresponding to the losses in the weak links between the grains shifts rapidly to lower T and its amplitude is strongly suppressed with increasing the dc magnetic field H{sub dc}, the other peaks related to the losses in the grains become visible. For the samples consisting of single phase 2223, these intragranular peaks are situated near 38 K, 58 K, and 80-88 K (depending on the sample), whereas for the samples consisting of single phase 2212, they are situated near 20 K, 43 K, and 77 K. For H{sub dc} {>=} 1 kOe, the temperature positions of the intragranular peaks are weakly field dependent. This shows that the transition line determined from the loss peaks behaves like the melting line in two-dimensional (2D) systems. Also, as the field increases, the amplitude of the loss peaks can decrease up to their disappearance, suggesting a layer decoupling process. In this way, we show that, above a crossover field of {approx}1 kOe, the vortex lattice in the superconducting grains of polycrystalline samples reproduces the behaviour of the vortex lattice in a quasi-2D object consisting of a set of separated 'thin films' with one-, two-, and three-effective-layer thicknesses. For the samples consisting of both 2223 and 2212 phases, three additional peaks were observed, situated near 28 K, 50 K and 75-80 K (depending on the sample). These peaks were attributed to the regions in which the 2212 and 2223 layers alternate.

  7. Tamarugite in the Steam-Condensate Alteration Paragenesis in Diana Cave (SW Romania)

    Science.gov (United States)

    Puscas, C. M.; Onac, B. P.; Effenberger, H. S.; Povară, I.

    2012-12-01

    The double-salt hydrate tamarugite [NaAl(SO4)2 6H2O] is an uncommon mineral in the cave environment, forming as a result of chemical reactions between water and bedrock only under very specific conditions. The Diana Cave hosts a unique tamarugite occurrence, the first one to be reported from a typical karst environment. The cave is located within the limits of Băile Herculane township in the Cerna Mountains, SW Romania. It consists of a 14 m long, westward-oriented single passage, developed along the Diana Fault. In 1974 a concrete-clad mine gallery was created to channel the thermal water (Diana 1+2 Spring) flowing through the cave to a pumping station. The spring's chemical and physical parameters fluctuated through time, averaging 51.98° C, discharge of 0.96 Ls-1, pH of 7.46, 5768.66 ppm TDS, 9303 μScm-1 conductivity, 5.02 salinity. The major chemical components of the thermo-mineral water in Diana Cave are, Na+ (1392.57 ppm), K+ (58.55 ppm), Ca2+ (725.16 ppm), Mg2+ (10.78 ppm), Cl- (3376.83 ppm), and SO42- (92.27 ppm), and H2S (24.05 ppm), with traces of Si, Fe2+, Br+, I-, and Li+. The general air circulation pattern within the cave is fairly simple: cold air from the outside sweeps into the cave along the floor, heats up at the contact with the thermo-mineral water, ascends, and exists the cave along the ceiling. At the contact with the cold walls of the Diana Cave, the hot steam condenses and gives rise to a rich and exotic sulfate-mineral paragenesis (including halotrichite-series minerals, gypsum, bassanite, anhydrite, epsomite, alunite, halite, native sulfur, etc.). The most exotic minerals precipitate at or below the contact between the Tithonic - Neocomian limestone and the overlaying Cretaceous shaly limestone, as a result of steam-condensate alteration. Minerogenetic mechanisms responsible for the peculiar sulfate mineral assemblage in Diana Cave are evaporation, oxidation, hydrolysis, double exchange reactions, and deposition from vapours or

  8. Reconstruction of External Auditory Meatus (Re-EAMs) and Tympanoplasty for、Patients with Congenital Deformation of the Middle and Outer Ears%先天性中外耳畸形外耳道再造与鼓室成形术

    Institute of Scientific and Technical Information of China (English)

    邹艺辉; 杨仕明; 戴朴; 韩维举; 韩东一

    2014-01-01

    Objective To explore the hearing improvement after reconstruction of external auditory meatus (Re-EAMs) and tympanoplasty for patients with congenital deformation of the middle and outer ears, and provide reference for later treat⁃ment to these kinds of patients. Methods a retrospective analysis of 41 cases (41 ears) with preoperative and postoperative hearing findings undergone Re-EAMs and tympanoplasty patients with congenital deformation of the middle and outer ears in General Hospital of PLA during 2006.7-2012.12. Results The methods for operations were Ossicular chain loosening 41.5%(17/41),Porp implantation 46.3%(19/41), Torp implantation 7.3%(3/41) and inner ear fenestration 4.9%(2/41),with average air conduction hearing threshold pre-and post-operation for 0.5k、1k、2k、4k (58 dB/53dB),(64 dB/52dB),(73 dB/73dB)and (62 dB/46dB)respectively. Conclusion Hearing improvement is not obvious after ossicular chain loosening and Torp implan⁃tation, while significant for Porp implantation and inner ear fenestration, but can’t reach the normal hearing threshold level.%目的:探索先天性中外耳畸形患者耳道再造与鼓室成形术后听力改善情况,为此类患者方案选择提供参考。方法回顾性分析2006年6月~2012年12月我们进行外耳道再造与鼓室成形患者中有术前术后听力的41例(41耳)的情况。结果手术采用听骨链松解41.5%(17/41),Porp植入46.3%(19/41),Torp植入7.3%(3/41),内耳开窗4.9%(2/41)。术前术后0.5k、1k、2k、4k平均气导听阈值分别为(58 dB/53dB),(64 dB/52dB),(73 dB/73dB),(62 dB/46dB).结论听骨链松解与Torp植入术后听力改善不明显,Porp植入和内耳开窗术后听力有明显改善,但未能达到正常听阈水平。