zebrafish pigment cells: Topics by WorldWideScience.org

Purpurin is a key molecule for cell differentiation during the early development of zebrafish retina

BackgroundThe zebrafish is recognized as a versatile cancer and drug screening model. However, it is not known whether the estrogen-responsive genes and signaling pathways that are...Full Text Available

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British Library Electronic Table of Contents (United Kingdom)

Recently, we cloned purpurin cDNA as an upregulated gene in the axotomized fish retina. The retina-specific protein was secreted from photoreceptors to ganglion cell layer during an early stage of optic nerve regeneration in zebrafish retina. The purpurin worked as a trigger molecule for axonal regrowth in adult injured fish retina. During zebrafish development, purpurin mRNA first appeared in ventral retina at 2 days post-fertilization (dpf) and spread out to the outer nuclear layer at 3 dpf. Here, we investigated the role of purpurin for zebrafish retinal development using morpholino gene knockdown technique. Injection of purpurin morpholino into the 1-2 cell stage of embryos significantly inhibited the transcriptional and translational expression of purpurin at 3 dpf. In the purpurin mo...

2009-01-01

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Zebrafish embryo extracts promote sphere-forming abilities of human melanoma cell line

British Library Electronic Table of Contents (United Kingdom)

Sphere-forming abilities in culture condition are considered a hallmark of cancer stem-like cells, which represents tumor cell invasiveness and stem-like characteristics. We aimed to show that the sphere-forming subpopulation of human malignant melanoma cell line WM-266-4 acts differently to zebrafish embryo extracts compared with their bulk counterpart. Spheres were maintained in neural stem cell culture conditions. The embryos of zebrafish at specific developmental stages were collected and the extracts were purified under 100 kDa. Spheres were treated with embyo extracts and proliferation assay and immunocytochemistry were conducted. Spheroid cells expressed nestin and epidermal growth factor receptor (EGFR) but not melanoma antigen recognized by T-cells (MART)1, indicating their stem-l...

2009-01-01

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Experimental Approaches to the Study of A2E, a Bisretinoid Lipofuscin Chromophore of Retinal Pigment Epithelium

nkx2.2a promotes specification and differentiation of a myelinating subset of oligodendrocyte lineage cells in zebrafish

Bisretinoid lipofuscin compounds that accumulate in retinal pigment epithelial (RPE) cells are implicated in the pathogenesis of some forms of macular degeneration. In the development of approaches...Full Text Available

2010-01-01

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Programmed cell death during pigment gland formation in Gossypium hirsutum leaves

During development, multipotent neural precursors give rise to oligodendrocyte progenitor cells (OPCs), which migrate and divide to produce additional OPCs. Near the end of embryogenesis and...Full Text Available

2008-05-01

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British Library Electronic Table of Contents (United Kingdom)

Abstract Ultrastructural studies have shown that the formation of pigment glands in Gossypium hirsutum L. leaves is a lysigenous process, originating from a cluster of cells in the ground meristem. Various techniques were used here to investigate whether programmed cell death (PCD) plays a critical role in this developmental process. Nuclei of internal cells in the pigment gland-forming tissue were TUNEL-positive and DAPI-negative, suggesting that DNA cleavage is an early event and complete DNA degradation is a late event. Smeared bands and a lack of laddering after gel electrophoresis indicate that DNA cleavage is random. Ultrastructurally, secretory cells in the pigment glands become distorted, nuclei are densely stained, and chromosomes become condensed until completely degraded at late...

2010-01-01

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Zebrafish TRPA1 Channels are Required for Chemosensation but not for Thermosensation or Mechanosensory Hair Cell Function

A highly conserved regulatory element controls hematopoietic expression of GATA-2 in zebrafish

Transient receptor potential (TRP) ion channels have been implicated in detecting chemical, thermal and mechanical stimuli in organisms ranging from mammals to Caenorhabditis elegans....Full Text Available

2008-10-01

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Model for phase III autografts of epidermal cells cultured on a collagen-proteoglycan biomatrix.

BackgroundGATA-2 is a transcription factor required for hematopoietic stem cell survival as well as for neuronal development in vertebrates. It has been shown that specific expression...Full Text Available

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Neuroprotectin D1/Protectin D1 Stereoselective and Specific Binding With Human Retinal Pigment Epithelial Cells and Neutrophils

The primary aim of this study was to develop a model system that uses epidermal cells (keratinocytes and accessory pigmented cells) cultured on a reconstituted basement membrane biomatrix for use in...Full Text Available

1989-10-01

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Transcription regulation of the vegf gene by the BMP/Smad pathway in the angioblast of zebrafish embryos

Retinal pigment epithelial (RPE) cells, derived from the neuroectoderm, biosynthesize the novel lipid mediator neuroprotectin D1 (NPD1) from docosahexaenoic acid (DHA) in response to oxidative...Full Text Available

2010-01-01

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Alpha particles for treatment of disseminated melanoma

Vascular endothelial growth factor (VEGF) is a mitogen that is critically involved in vasculogenesis, angiogenesis, and hematopoiesis. However, what and how transcription factors participate in the regulation of vegf gene expression are not fully understood. Here we report the cloning and sequencing of the zebrafish vegf promoter which revealed that the promoter contains a number of bone morphogenetic protein (BMP)-activated Smad binding elements (SBE), implicating Smad1 and Smad5 in the regulation of BMP-induced expression of vegf. Electrophoretic mobility shift assays of adding recombinant Smad proteins to the SBE-containing DNA oligonucleotides that represent portions of zebrafish vegf promoter resulted in mobility shift of the oligonucleotides. These changes demonstrate potential interactions between Smad1/5 and the vegf promoter. Reporter activity assays using the wild-type or SBE-deleted vegf promoters to drive the luciferase reporter ...

2005-04-01

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A2e mediated phototoxic effects of endoilluminators

Invading melanoma spreads to local and unpredictable distant location at the early stages of its development. It is justifiable, therefore, to classify the disease as a systemic disorder. This requires a systemic treatment that reaches all melanoma cells irrespective of whether they are singly dispersed and in circulation or already forming solid tumours of various sizes. Targeted radiotherapy affects directly and selectively cancer cells provided an appropriate radionuclide and its carrier are chosen. Melanoma is a pigmented tumour. Methylene blue (MTB)) accumulates selectively in melanoma cells due to its exceptionally high affinity to melanin. MTB serves, therefore, as a carrier for radionuclides. "2"1"1At-MTB has proved to be particularly effective in treating disseminated melanoma when administered systemically and, at the same time, non-toxic to normal non-pigmented and ...

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Antenna organization in green photosynthetic bacteria. Progress report, March 1986--February 1987

AimsTo compare the theoretical retinal threshold time for endoilluminators and experimental phototoxic effect using A2e laden retinal pigment epithelial (RPE) cells.MethodsThe...Full Text Available

2006-02-01

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Celastrol regulates innate immunity response via NF-@kB and Hsp70 in human retinal pigment epithelial cells

This project is concerned with the structure and function of the unique antenna system found in the green photosynthetic bacteria. The antenna system in these organisms is contained within a vesicle known as a chlorosome, which is attached to the cytoplasmic side of the cell membrane. Additional antenna pigments and reaction centers are contained in integral membrane proteins. Energy absorbed by the bacteriochlorophyll c (BChl c) pigments in the chlorosome is transferred via a ``baseplate`` array of BChl a antenna pigments into the membrane and to the reaction center. This system is similar in some respects to the phycobilisome antenna system found in cyanobacteria and some types of algae, in that a membrane-associated structure absorbs light and transfers it to the membrane where conversion to chemical energy takes place. However, the overall structure, the type of pigments ...

1987-12-31

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British Library Electronic Table of Contents (United Kingdom)

Elevated nuclear factor kappa B (NF-@kB) activity and interleukin-6 (IL-6) secretion participates in the pathology of several age and inflammatory-related diseases, including age-related macular degeneration (AMD), in which retinal pigment epithelial cells are the key target. Recent findings reveal that heat shock protein 70 (Hsp70) may affect regulation of NF-@kB. In the current study, effects of Hsp70 expression on NF-@kB RelA/p65 activity were evaluated in human retinal pigment epithelial cells (ARPE-19) by using celastrol, a novel anti-inflammatory compound. Anti-inflammatory properties of celastrol were determined by measuring expression levels of IL-6 and endogenous NF-@kB levels during lipopolysaccharide (LPS) exposure by using enzyme-linked immunosorbent assays (ELISA). Cell viabil...

2011-01-01

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PI3K/Akt and mTOR/p70S6K Pathways Mediate Neuroprotectin D1-Induced Retinal Pigment Epithelial Cell Survival during Oxidative Stress-Induced Apoptosis

HSP70, the earliest-induced gene in the zebrafish retina during optic nerve regeneration: Its role in cell survival

The initiation and progression of several forms of retinal degenerations involve excessive, repetitive, and/or sustained oxidative stress that, in turn, mediate photoreceptor cell damage and...Full Text Available

2010-06-01

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British Library Electronic Table of Contents (United Kingdom)

Fish retinal ganglion cells (RGCs) can survive and regrow their axons after optic nerve injury. Injured RGCs express anti-apoptotic proteins, such as Bcl-2, after nerve injury; however, upstream effectors of this anti-apoptotic protein are not yet fully understood. Heat shock proteins (HSPs) play a crucial role in cell survival against various stress conditions. In this study, we focused on HSP70 expression in the zebrafish retina after optic nerve injury. HSP70 mRNA and protein levels increased rapidly 2.3-fold in RGCs by 1-6 h after injury and returned to control levels by 1-3 days. HSP70 transcription is regulated by heat shock factor 1 (HSF1). HSF1 mRNA and phosphorylated-HSF1 protein rapidly increased by 2.2-fold in RGCs 0.5-6 h after injury. Intraocular injection of HSP inhibitor I s...

2011-01-01

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Defective Glycinergic Synaptic Transmission in Zebrafish Motility Mutants

NEW METHOD FOR PRODUCING PIGMENT NANOPARTICLE

Glycine is a major inhibitory neurotransmitter in the spinal cord and brainstem. Recently, in vivo analysis of glycinergic synaptic transmission has been pursued in zebrafish using...Full Text Available

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J-STORE (Japan)

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2004-05-14

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Malignancy without immortality? Cellular immortalization as a possible late event in melanoma progression

British Library Electronic Table of Contents (United Kingdom)

Summary Cell senescence is a permanent growth arrest following extended proliferation. Cultured cancer cells including metastatic melanoma cells often appear immortal (proliferate indefinitely), while uncultured benign nevi (moles) show senescence markers. Here, with new explantation methods, we investigated which classes of primary pigmented lesions are typically immortal. Nevi yielded a few proliferating cells, consistent with most nevus cells being senescent. No nevus culture (0/28) appeared immortal. Some thin and thick melanoma cultures proved immortal under these conditions, but surprisingly few (4/37). All arrested cultures displayed three senescence markers in some cells: -galactosidase, nuclear p16, and heterochromatic foci/aggregates. However, melanoma cultures also showed featur...

2011-01-01

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Comparative action spectrum for ultraviolet light killing of mouse melanocytes from different genetic coat color backgrounds

The Effect of Static Magnetic Forces on Water Contents and Photosynthetic Pigments in Sweet Basil Ocimum basilicum L. (Lamiaceae)

The photobiology of mouse melanocyte lines with different pigment genotypes was studied by measuring colony-forming ability after irradiation. The cell lines were wild-type black (melan-a) and the mutants brown (melan-b) and albino (melan-c). Four lamps emitting various UV wavelengths were used. These were germicidal (UVC, 200-280 nm), 82.3% output at 254 nm, TL01 (UVB, 280-320 nm), 64.2% at 310-311 nm, FS20, broadband with peak output at 312 nm and Alisun-S (UVA, 320-400 nm), broadband with peak output at 350-354 nm. Appropriate filtration reduced the contaminating UVC to nonlethal levels for the longer waverange lamps. It is clear from these studies that, in pigment cells, monochromatic results cannot predict polychromatic responses and that cell death from solar irradiations is a complex phenomenon that depends on more than DNA damage. (author).

1997-01-01

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Elastic Properties of Nematic Liquid Crystals Formed by Living and Migrating Cells

Three different magnetic regimes; aerial, surface and buried; each with three different forces, have been used to investigate their effects on the water contents and photosynthetic pigments of sweet basil plants (Ocimum basilicum L.). Two groups of sweet basil seeds, Ocimum basilicum L. have been cultivated, one under normal conditions and the second has been subdivided into three portion (aerial, surface and buried) to examine the effect of different magnetic forces coming from the three directions on the resulted plants. At all directions of magnets, water contents have been significantly affected by the magnetic forces. Chlorophyll A and carotene contents have been affected, as well, according to the three magnetic forces coming from soil surface regime only. Chlorophyll B did not significantly affected by differences magnetic forces in the three regimes, but it is affected by magnetism wherever its direction or force. But all the photosynthetic ...

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The Formation of Anthocyanic Vacuolar Inclusions in Arabidopsis thaliana and Implications for the Sequestration of Anthocyanin Pigments

In culture migrating and interacting amoeboid cells can form nematic liquid crystal phases. A polar nematic liquid crystal is formed if the interaction has a polar symmetry. One type of white blood cells (granulocytes) form clusters where the cells are oriented towards the center. The core of such an orientational defect (disclination) is either a granulocyte forced to be in an isotropic state or another cell type like a monocyte. An apolar nematic liquid crystal is formed if the interaction has an apolar symmetry. Different cell types like human melanocytes (=pigment cells of the skin), human fibroblasts (=connective tissue cells), human osteoblasts (=bone cells), human adipocytes (= fat cells) etc., form an apolar nematic liquid crystal. The orientational elastic energy is ...

1998-01-01

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Zebrafish: A Model System for the Study of Eye Genetics

Anthocyanins are flavonoid pigments that accumulate in the large central vacuole of most plants. Inside the vacuole, anthocyanins can be found uniformly distributed or as part of sub-vacuolar pigment...Full Text Available

2010-01-01

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PRELIMINARY STUDY ON MODIFICATION OF YOLK SAC OF ZEBRAFISH EMBRYOS (DANIO RERIO) USING MICROINJECTION

Over the last decade, the use of the zebrafish as a genetic model has moved beyond the proof-of-concept for the analysis of vertebrate embryonic development to demonstrated utility as a mainstream...Full Text Available

2008-01-01

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Duplication and Diversification of the Hypoxia-Inducible IGFBP-1 Gene in Zebrafish

Low membrane permeability is one of the major obstacles to the successful cryopreservation of zebrafish embryos. The aim of the present study was to explore if this could be overcome by yolk...Full Text Available

2006-01-01

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Construction and Application of a Zebrafish Array CGH Platform

BackgroundGene duplication is the primary force of new gene evolution. Deciphering whether a pair of duplicated genes has evolved divergent functions is often challenging. The zebrafish...Full Text Available

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Analysis of the astray/robo2 zebrafish mutant reveals that degenerating tracts do not provide strong guidance cues for regenerating optic axons

The zebrafish is emerging as a prominent model system for studying the genetics of human development and disease. Genetic alterations that underlie each mutant model can exist in the form of...Full Text Available

2009-02-01

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Cadmium inhibits neurogenesis in zebrafish embryonic brain development

During formation of the optic projection in astray/robo2 mutant zebrafish, optic axons exhibit rostro-caudal pathfinding errors, ectopic midline crossing and...Full Text Available

2010-10-13

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Pigment Analysis by HPLC at Horn Point Laboratory

Cadmium is a non-essential heavy metal found abundantly in the environment. Children of women exposed to cadmium during pregnancy display lower motor and perceptual abilities. High cadmium body burden in children is also related to impaired intelligence and lowered school achievement. However, little is known about the molecular and cellular basis of developmental neurotoxicity in the sensitive early life stages of animals. In this study, we explore neurological deficits caused by cadmium during early embryonic stages in zebrafish by examining regionalization of the neural tube, pattern formation and cell fate determination, commitment of proneural genes and induction of neurogenesis. We show that cadmium-treated embryos developed a smaller head with unclear boundaries between the brain subdivisions, particularly in the mid-hindbrain region. Embryos display normal anterior to posterior regionalization; however, the commitment of neural ...

2008-05-01

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HPLC Phytoplankton Pigment Measurements ... - Ocean Color - NASA

Oct 30, 2006 ... Pigment Analysis by HPLC at Horn Point Laboratory. Laurie Van Heukelem. Crystal Thomas. Meg Maddox. University of Maryland Center for ...

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Organ culture of mammalian skin and the effects of ultraviolet light and testosterone on melanocyte morphology and function

CHORS, SDSU. OCRT Meeting (Washington, D.C., 14-16 Apr 2004). HPLC Phytoplankton Pigment Measurements: Continuation of Climate Quality Data Records ...

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Mapping of the gene encoding the melanocortin-1 ([alpha]-melanocyte stimulating hormone) receptor (MC1R) to human chromosome 16q24. 3 by fluorescence in situ hybridization

Scrotal skin of black Long-Evans rats and human thigh skin were maintained in vitro as organ cultures for as long as 14 days, and examined histologically using the combined skin splitting and Dopa techniques. Selected rat skin cultures received testosterone in the culture medium and/or were irradiated with ultraviolet light (290 to 320 nm uvl). With increased time in culture, scrotal melanocytes round up and there is an increase in epidermal pigmentation. Human skin behaves similarly; after eight days in vitro human melanocytes also become rounded, but remain strongly Dopa-positive. Addition of exogenous testosterone to cultured rat skin maintains dendritic morphology of melanocytes, but cell body size is still reduced. uvl irradiation stimulates melanocytes in rat skin cultures, maintaining their dendritic morphology and increasing epidermal and dermal pigmentation. Cultured skin receiving both uvl and testosterone ...

1978-05-01

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Effects of Simulated Acid Rain on Growth, Pigments and Leaf Surface Morphology of the Seedlings of Amaranthus tricolor L.

[alpha]-Melanocyte stimulating hormone ([alpha]-MSH), a hormone originally named for its ability to regulate pigmentation of melanocytes, is a 13-amino-acid post-translational product of the pro-opiomelanocortin (POMC) gene. [alpha]-MSH and the other products of POMC processing, which share the core heptapeptide amino acid sequence Met-Glu (Gly)-His-Phe-Arg-Trp-Gly (Asp), the adrenocorticotropic hormone (ACTH), [beta]-MSH, and [gamma]-MSH, are collectively referred to as melanocortins. While best known for their effects on the melanocyte (pigmentation) and adrenal cortical cells (steroidogenesis), melanocortins have been postulated to function in diverse activities, including enhancement of learning and memory, control of the cardiovascular system, analgesia, thermoregulation, immunomodulation, parturition, and neurotrophism. To identify the chromosomal band encoding the human melanocortin-1 receptor gene, 1 [mu]g of an ...

1994-01-15

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The neural basis of visual behaviors in the larval zebrafish

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1994-08-01

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British Library Electronic Table of Contents (United Kingdom)

We review visually guided behaviors in larval zebrafish and summarise what is known about the neural processing that results in these behaviors, paying particular attention to the progress made in the last 2 years. Using the examples of the optokinetic reflex, the optomotor response, prey tracking and the visual startle response, we illustrate how the larval zebrafish presents us with a very promising model vertebrate system that allows neurocientists to integrate functional and behavioral studies and from which we can expect illuminating insights in the near future.

2009-01-01

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Molecular cloning of the human homeobox gene goosecoid (GSC) and mapping of the gene to human chromosome 14q32. 1

Visual acuity in larval zebrafish: behavior and histology

Goosecoid is a homeobox gene first isolated from a Xenopus dorsal lip cDNA library. Homologous genes have been isolated from mouse, zebrafish, and chick. In all species examined, the gene is expressed and plays an important role during the process of gastrulation in early embryonic development. The authors report here the cloning of the human goosecoid (GSC) from a genomic library and the sequence of its encoded protein. The genomic organization and protein sequence of the human gene are highly conserved with respect to those of its Xenopus and mouse counterparts: all three genes consist of three exons, with conserved exon-intron boundaries. The sequence of the homeo-domain is 100% conserved in most vertebrates. Using somatic cell hybrid and chromosomal in situ hybridization, the gene was mapped to chromosome 14q32.1. 30 refs., 3 figs., 2 tabs.

1994-05-15

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Embryonic Senescence and Laminopathies in a Progeroid Zebrafish Model

BackgroundVisual acuity, the ability of the visual system to distinguish two separate objects at a given angular distance, is influenced by the optical and neuronal properties of...Full Text Available

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Using Regulatory and Epistatic Networks to Extend the Findings of a Genome Scan: Identifying the Gene Drivers of Pigmentation in Merino Sheep

BackgroundMutations that disrupt the conversion of prelamin A to mature lamin A cause the rare genetic disorder Hutchinson-Gilford progeria syndrome and a group of laminopathies....Full Text Available

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Short and long term axotomy-induced ERG changes in albino and pigmented rats

Extending genome wide association analysis by the inclusion of gene expression data may assist in the dissection of complex traits. We examined piebald, a pigmentation phenotype in both human and Merino...Full Text Available

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Chronobiological analysis and mass spectrometric characterization of pigment-dispersing factor in the cockroach Leucophaea maderae

PurposeTo investigate the different components of full-field flash electroretinogram (ERG) responses in adult albino and pigmented rats at various time intervals following optic...Full Text Available

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Characterization of a pigment-dispersing hormone in eyestalks of the fiddler crab Uca pugilator

Pigment-dispersing factor (PDF) is a neuropeptide that plays a prominent role in the circadian clock of several insects. The cockroach Leucophaea maderae...Full Text Available

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Macular Pigment Density Evolution in Unilateral Wet AMD Versus Non AMD Patients With or Without Lutein and Zeaxanthine Supplementation

A pigment-dispersing hormone (PDH) from eyestalks of the fiddler crab Uca pugilator has been purified by gel filtration, ion-exchange chromatography, partition chromatography, and reversed-phase...Full Text Available

1985-08-01

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Functional Characterization of Melanocyte Stem Cells in Hair Follicles.

Exudative Age-related Macular Degeneration

2011-08-03

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Synthesis, characterization and optical properties of a high NIR reflecting yellow inorganic pigment: Mo"6"+ doped Y_2Ce_2O_7 as a cool colorant

In mice, coat pigmentation requires a stem cell (SC) system in which the survival, proliferation, and differentiation of melanocytes (MCs) are regulated by microenvironments in hair follicles (HFs). In vitro systems are required to analyze the behavior of single melanocyte stem cells (MCSCs) and their potential to form SC systems in vivo. We describe here an experimental system for the isolation, self-renewal, and differentiation of MCSCs, as well as an in vivo reconstitution assay for assessing their potential. Using Dct(tm1(Cre)Bee)/CAG-CAT-GFP mice, we show that, in the presence of stem cell factor and basic fibroblast growth factor and the XB2 feeder cell line, purified MCSCs can undergo clonogenic proliferation, resulting in c-Kit(low) side scatter(low) cells. In culture, these cells maintain their capacity to differentiate and ...

2011-07-14

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Investigations of biomimetic light energy harvesting pigments

Full text: Pigments possessing the ability to confer high solar reflectance have received considerable attention in recent years. The inorganic class of NIR reflective pigments are mainly metal oxides and are primarily employed in two applications: (i) visual camouflage and (ii) reducing heat build up. More than half of the solar radiation consists of near-infrared radiation (52%), the remaining being 43% visible light and 5% ultraviolet radiation. Over heating due to solar radiation negatively affects comfort in the built environment and contributes substantially to electrical consumption for air conditioning and release of green house gases. A pigment which has strong reflections in the NIR region (780-2500 nm) can be referred to as a 'cool' pigment. However, most of the NIR reflective inorganic pigments particularly yellow (eg. cadmium yellow, lead chromate, chrome titanate ...

2010-11-24

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misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance

This is the final report of a three-year, Laboratory Directed Research and Development (LDRD) project at Los Alamos National Laboratory (LANL). Nature uses chlorophyll and other porphyrinic pigments to capture and transfer light energy as a preliminary step in photosynthesis. The design of synthetic assemblies of light harvesting and energy directing pigments has been explored through synthesis and characterization of porphyrin oligomers. In this project, pigment electronic and vibrational structures have been explored by electrochemistry and dynamic and static optical measurements. Transient absorption data reveal energy transfer between pigments with lifetimes on the order of 20--200 picoseconds, while Raman data reveal that the basic porphyrin core structure is unperturbed relative to the individual monomer units. These two findings, along with an extensive series of experiments on the oxidized ...

1998-12-01

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Transposable elements in fish functional genomics: technical challenges and perspectives

Somite boundary formation is crucial for segmentation of vertebrate somites and vertebrae and skeletal muscle morphogenesis. Previously, we developed a Tol2 transposon-mediated...Full Text Available

2008-04-15

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The zebrafish heart regenerates after cryoinjury-induced myocardial infarction

The recent introduction of several transposable elements in zebrafish opens new frontiers for genetic manipulation in this important vertebrate model. This review discusses transposable elements as...Full Text Available

2007-01-01

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The rapid generation of chimerical genes expanding protein diversity in zebrafish

BackgroundIn humans, myocardial infarction is characterized by irreversible loss of heart tissue, which becomes replaced with a fibrous scar. By contrast, teleost fish and urodele...Full Text Available

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Shared versus specialized glycinergic spinal interneurons in axial motor circuits of larval zebrafish

BackgroundVariation of gene number among species indicates that there is a general process of new gene origination. One of the major mechanism providing raw materials for the origin...Full Text Available

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Sequence and expression of the zebrafish alpha-actinin gene family reveals conservation and diversification among vertebrates

The neuronal networks in spinal cord can produce a diverse array of motor behaviors. In aquatic vertebrates such as fishes and tadpoles, these include escape behaviors, swimming across a range...Full Text Available

2008-11-26

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PGE2-regulated wnt signaling and N-acetylcysteine are synergistically hepatoprotective in zebrafish acetaminophen injury

alpha-actinins are actin microfilament crosslinking proteins. Vertebrate actinins fall into two classes: the broadly-expressed actinins 1 and 4 (actn1 and actn4)...Full Text Available

2009-11-01

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Myofibrillogenesis in the developing zebrafish heart: A functional study of tnnt2

Acetaminophen (APAP) toxicity is the most common drug-induced cause of acute liver failure in the United States. The only available treatment, N-acetylcysteine (NAC), has a limited...Full Text Available

2010-10-05

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Knockdown of Bicaudal C in Zebrafish (Danio rerio) Causes Cystic Kidneys: A Nonmammalian Model of Polycystic Kidney Disease

Various hypotheses have been proposed to explain the molecule processes of sarcomere assembly, partially due to the lack of systematic genetic studies of sarcomeric genes in an in vivo...Full Text Available

2009-07-15

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Identification of a distant cis-regulatory element controlling pharyngeal arch-specific expression of zebrafish gdf6a/radar

Polycystic kidney disease (PKD) is one of the leading causes of end-stage renal disease in humans and is characterized by progressive cyst formation, renal enlargement, and abnormal tubular development....Full Text Available

2010-04-01

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Efficient discovery of ASCL1 regulatory sequences through transgene pooling

Skeletal formation is an essential and intricately regulated part of vertebrate development. Humans and mice deficient in Growth and Differentiation Factor 6 (Gdf6) have numerous...Full Text Available

2010-04-01

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A genome-wide survey of Major Histocompatibility Complex (MHC) genes and their paralogues in zebrafish

Zebrafish transgenesis is a powerful and increasingly common strategy to assay vertebrate transcriptional regulatory control. Several challenges remain, however, to the broader application of...Full Text Available

2010-06-01

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One-electron oxidation of photosynthetic pigments in micelles. Bacteriochlorophyll a, chlorophyll a, chlorophyll b, and pheophytin a

BackgroundThe genomic organisation of the Major Histocompatibility Complex (MHC) varies greatly between different vertebrates. In mammals, the classical MHC consists of a large number...Full Text Available

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abstract - 2008 NASA Carbon Cycle and Ecosystems Joint Science ...

Chlorophyll a, chlorophyll b, and bacteriochlorophyll a in aqueous micellar solutions of Trition X 100 (2%) are readily oxidized by pulse-radiolytically generated N_3., Br_2"-., and (SCN)_2"-. radicals at nearly diffusion-controlled rates. The kinetic study suggests that pigment molecules occupy multiple sites in the micelle. Pheophytin a is only oxidized by N_3. and Br_2"-. radicals. The absolute spectra and the molar extinction coefficients of chlorophyll a, bacteriochlorophyll a, chlorophyll b, and pheophytin a cations have been determined. The chlorophyll a cation has been observed in the presence of pigment aggregates.

1981-11-01

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Gpnmb is a Melanoblast-Expressed, MITF-Dependent Gene

Microscopy and chemotaxonomy from HPLC analysis of phytoplankton pigments initiated in 2003 suggest that Noctiluca miliaris blooms are becoming more intense ...

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GENETIC STUDIES OF PIGMENTATION IN ...

SUMMARYExpression profile analysis clusters Gpnmb with known pigment genes, Tyrp1, Dct, and Si. During development,...Full Text Available

2009-02-01

63

Enhancement of Keratinocyte Differentiation by Rose Absolute Oil

... Strains Smith and -aybush were both grown in broth containing from 0.1 to 50 pg/ml of acriflavin, acridine orange, or proflavin. ...

1966-05-01

64

Chromosomal rearrangement segregating with adrenoleukodystrophy: A molecular analysis

BackgroundThrough differentiation processes, keratinocytes provide a physical barrier to our bodies and control skin features such as moisturization, wrinkles and pigmentation. Keratinocyte...Full Text Available

2010-08-01

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Detecting Sugarcane yellow leaf virus infection in asymptomatic leaves with hyperspectral remote sensing and associated leaf pigment analysis

The relationship between X chromosome-linked adrenoleukodystrophy and the red/green color pigment gene cluster on Xq28 was investigated in a large kindred. The DNA in a hemizygous male showed altered restriction fragment sizes compatible with at least a deletion extending from the 5[prime] end of the color pigment genes. Segregation analysis using a DNA probe within the color pigment gene cluster showed significant linkage with adrenoleukodystrophy (logarithm of odds score of 3.19 at [theta] = 0.0). These data demonstrate linkage, rather than association, between a unique molecular rearrangement in the color pigment gene cluster and adrenoleukodystrophy. The DNA changes in this region are thus likely to be helpful for determining the location and identity of the responsible gene. 33 refs., 4 figs.

1993-10-15

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Development of a pozzolanic pigment from red mud

Sugarcane yellow leaf caused by Sugarcane yellow leaf virus (ScYLV) does not produce visual symptoms in most susceptible plants until late in the growing season. An experiment was conducted to determine if leaf reflectance and pigment analysis could be used to determine ScYLV infection prior to sym...

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Activation of a multisensory, multifunctional nucleus in the zebrafish midbrain during diverse locomotor behaviors

Red mud is a waste generated by the aluminum industry, and its disposal is a major problem for this industry. Very rich in iron, it can be used as cheap pigment for colored concrete. The red coloration can be enhanced by calcination in the range of 600 to 800 C. Such operation also transforms the aluminum hydroxides (goethite and boehmite) and clays minerals into pozzolanic admixtures that are able to consume the calcium hydroxide produced by cement hydration. Thus, it is possible to develop a new admixture for concrete: a pozzolanic pigment. The pozzolanic properties of calcined red mud were investigated by monitoring lime consumption of different mixtures of OPC and red mud. The main products of hydration were C-S-H and mono-carboaluminate (C{sub 4}A{bar C}H{sub 11}). A uniform and durable colored concrete was obtained using white cement interground with 11% of burnt red mud.

1997-10-01

68

British Library Electronic Table of Contents (United Kingdom)

Action potentials from the brain control the activity of spinal neural networks to produce, by as yet unknown mechanisms, a variety of motor behaviors. Particularly lacking are details on how identified descending neurons integrate diverse sensory inputs to generate specific locomotor patterns. We have examined the operations of the principal neurons in an intriguing midbrain nucleus, the nucleus of the medial longitudinal fasciculus (nMLF), in the larval zebrafish. The nMLF is the most rostral grouping of neurons that projects from the brain well into the spinal cord of teleost fishes, yet there is little direct physiological data available regarding its function. We report here that a distinct set of large, individually-identifiable neurons in nMLF (the MeL and MeM neurons) are activated...

2010-01-01

69

Transduction noise induced by 4-hydroxy retinals in rod photoreceptors.

The red-green visual pigment gene region in adrenoleukodystrophy.

New visual pigments were formed with 4-hydroxy retinals in isolated vertebrate rod photoreceptors by exposing bleached rods from the tiger salamander, Ambystoma tigrinum, to lipid vesicles containing...Full Text Available

1990-01-01

70

The determination of hemoglobin and myoglogin residues as a parameter for testing heat exposure in back bacon.

Although recent data established that a specific very-long-chain fatty acyl-CoA synthetase is defective in X-linked adrenoleukodystrophy (ALD), the ALD gene is still unidentified. The ALD locus has...Full Text Available

1990-03-01

71

SeaWiFS Postlaunch Technical Report Series

The use of an extraction of the heme pigments hemoglobin and myoglobin as a test for the heat exposure of back bacon was investigated by treating back bacon at varying temperatures of 50-70 degrees...Full Text Available

1976-07-01

72

Isolation and characterization of a new Cytophaga species implicated in a work-related lung disease.

tion and the Copenhagen symposium in many regards heralded the birth of modern marine bio-optics and the .... given to conducting a pigment intercomparison based on .... assignment of personnel and equipment, so the Hout Bay deployments were suspended. ...... submarine light field. Upwelling radiance is measured at ...

73

Inhibition of the visual cycle by A2E through direct interaction with RPE65 and implications in Stargardt disease

A yellow-pigmented, gram-negative, gliding bacterium isolated from an industrial water spray air humidification system was implicated as a causative agent in several occurrences of lung disease with...Full Text Available

1984-11-01

74

Hair Follicle Pigmentation

Stargardt disease (STGD) is the major form of inherited juvenile macular degeneration. Pyridinium bis-retinoid A2E is a major component of lipofuscin which accumulates in retinal pigment epithelium...Full Text Available

2010-10-12

75

Evidence of an inflammatory-like response in non-normally pigmented tissues of two scleractinian corals

Hair shaft melanin components (eu- or/and pheomelanin) are a long-lived record of precise interactions in the hair follicle pigmentary unit, e.g., between follicular melanocytes, keratinocytes,...Full Text Available

2005-01-01

76

Ceramics Thermosynthesis in Combustion Wave

Increasing evidence of links between climate change, anthropogenic stress and coral disease underscores the importance of understanding the mechanisms by which reef-building corals resist infection...Full Text Available

2008-12-07

77

International Science & Technology Center (ISTC)

Development of New Resource-Saving Technologies for Ceramic Material Production: High resistant Pigments, Heat Shielding, Plasters, Filters, etc.; by the Method of Controllable Thermosynthesis in Combustion Wave Using Industrial Metal-Containing Wastes

78

Antibacterial Colorants: Characterization of Prodiginines and Their Applications on Textile Materials

Aging of the Hair Follicle Pigmentation System

A strain of Vibrio sp. isolated from marine sediments produced large quantities of bright red pigments that could be used to dye many fibers including wool, nylon, acrylics,...Full Text Available

2008-05-01

79

Moessbauer spectroscopy study of iron corrosion underneath painting system

Skin and hair phenotypes are powerful cues in human communication. They impart much information, not least about our racial, ethnic, health, gender and age status. In the case of the latter parameter,...Full Text Available

2009-07-01

80

Computing with cells advances in membrane computing

The effect of pigments on the development of corrosion products between the painting system and metal surface when exposed to marine environments has been discussed. The pigments studied were: red mud zinc chromate, zinc chromate, red oxide zinc phosphate, manganese phosphate barium chromate and basic lead silico chromate. Moessbauer spectroscopy revealed that the upper rust layer in all the cases consisted of #gamma#-Fe_2O_3, #gamma#-FeOOH and #alpha#-FeOOH. The lower rust layer immediately in contact with the metal surface consisted of an asymmetrical doublet due to #gamma#-FeOOH. (Auth.).

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81

Spectroscopic study of some historical arts

Computing with cells

2009-01-01

82

This work presents the spectroscopic results of the investigations of ancient pottery remains excavated in ancient Kremna (BURDUR-TURKEY). The micro-Raman and FT-IR spectroscopic studies of the pottery remains belonging to sixth century allowed us to identify the mineral composition. Some examples are described of the use of this technique in the field of art conservation and diagnostics, with regard to pigments, and some products of metal alteration.

2009-09-24

83

News from Smithers Rapra

Molecular and functional characterization of a novel chromoplast-specific lycopene ?-cyclase from Citrus and its relation to lycopene accumulation

...of Mixing Sections 9 Interacting Rotor/Stator Mixers 10 Floating Ring Mixing Devices 11 Static (or Motionless) Mixers 12 Incorporation of Liquid Additives and Dispersions by Direct Addition 13 Dispersive Mixing of Fillers and Pigments 14 Dispersive Mixing Applied to Polymer Blending 15 Compounding with Single Screw Extruders Appendix - Preparation of Microtome Sections for Assessment of Dispersive In order to get a feel for this book, you can view Chapter 1 now, simply follow the link ...

84

Mechanism for the anti-thyroid action of minocycline

Carotenoids are the main pigments responsible of the colouration of Citrus fruits. The β-cyclization of lycopene, catalysed by the lycopene β-cyclases (β-LCY),...Full Text Available

2009-04-01

85

Lamp system for uniform semiconductor wafer heating

Administration of minocycline (MN), a tetracycline antibiotic, produces a black pigment in the thyroids of humans and several species of experimental animals and antithyroid effects in rodents. We have previously shown that these effects appear to be related to interactions of MN with thyroid peroxidase (TPO), the key enzyme in thyroid hormone synthesis. In the present study, the mechanisms for inhibition of TPO-catalyzed iodination and coupling reactions by MN were investigated. 37 refs., 7 figs., 3 tabs.

1997-01-01

86

ECRbase: Database of Evolutionary Conserved Regions, Promoters, and Transcription Factor Binding Sites in Vertebrate Genomes

A lamp system with a very soft high-intensity output is provided over a large area by water cooling a long-arc lamp inside a diffuse reflector of polytetrafluorethylene (PTFE) and titanium dioxide (TiO.sub.2) white pigment. The water is kept clean and pure by a one micron particulate filter and an activated charcoal/ultraviolet irradiation system that circulates and de-ionizes and biologically sterilizes the coolant water at all times, even when the long-arc lamp is off.

2001-01-01

87

Evaluation of a convenient method of assessing rodent visual function in safety pharmacology studies: Effects of sodium iodate on visual acuity and retinal morphology in albino and pigmented rats and mice

Evolutionary conservation of DNA sequences provides a tool for the identification of functional elements in genomes. We have created a database of evolutionary conserved regions (ECRs) in vertebrate genomes entitled ECRbase that is constructed from a collection of pairwise vertebrate genome alignments produced by the ECR Browser database. ECRbase features a database of syntenic blocks that recapitulate the evolution of rearrangements in vertebrates and a collection of promoters in all vertebrate genomes presented in the database. The database also contains a collection of annotated transcription factor binding sites (TFBS) in all ECRs and promoter elements. ECRbase currently includes human, rhesus macaque, dog, opossum, rat, mouse, chicken, frog, zebrafish, and two pufferfish genomes. It is freely accessible at http://ECRbase.dcode.org.

2006-08-08

88

British Library Electronic Table of Contents (United Kingdom)

Introduction: We have evaluated the ability of a semi-automated, optomotor reflex method to assess drug-induced visual dysfunction, in albino and pigmented rats and mice. Methods: Male Han Wistar (HW) and Long Evans (LE) rats and mice (CD-1 and C57BL/6) were tested in a chamber formed by 4 computer monitors displaying a rotating vertical grating, to elicit head-tracking movements. The highest visible grating frequency was taken as the threshold of visual acuity, in cycles per degree (c/d). Animals received an intravenous infusion of either sodium iodate (50mg/kg) or 0.9% w/v NaCl (aq). They were tested 2h later, then re-tested daily for a further 3days. The time course of the effect was assessed in HW rats over a 6-week period, including electron microscopy, and immunohistochemical analysi...

2011-01-01

89

Toxicity: Polymeric materials in food-contact applications. January 1977-September 1989 (Citations from the Rubber and Plastics Research Association data base). Report for January 1977-September 1989

Manufacturing of golf club using wood-plastic combination produced by. gamma. -irradiation

This bibliography contains citations concerning toxicity investigations of polymeric materials in food-contact applications. Polymeric food-packaging materials and regulations are discussed. Toxicity testing, polymeric equipment in food processing, and the use of additives in food packaging are included. Discussions also include coating materials for food containers and pigments for food packaging films. (This updated bibliography contains 275 citations, 10 of which are new entries to the previous edition.)

1989-09-01

90

Manufacturing of golf club using wood-plastic combination produced by #gamma#-irradiation

Wood-plastic combination (WPC) was produced by {gamma}-irradiation of persimmon impregnated with acrylonitrile and styrene. The hardness and strength of WPC obtained were higher than those of an unmodified wood. Thus, it was found that the WPC is suited for a head of golf club, because the Shore hardness value of WPC is 36% greater than that of unmodified wood. An impregnation method of monomers with some pigments could produce colored WPC without diminishing natural grain. Head of golf club could be manufactured from colored WPC in practice. (auhtor).

1992-01-01

91

FUEL CELL AND FUEL CELL SYSTEM

Wood-plastic combination (WPC) was produced by #gamma#-irradiation of persimmon impregnated with acrylonitrile and styrene. The hardness and strength of WPC obtained were higher than those of an unmodified wood. Thus, it was found that the WPC is suited for a head of golf club, because the Shore hardness value of WPC is 36% greater than that of unmodified wood. An impregnation method of monomers with some pigments could produce colored WPC without diminishing natural grain. Head of golf club could be manufactured from colored WPC in practice. (auhtor).

1992-01-01

92

J-STORE (Japan)

Full Text Available

2008-12-12

93

Thin Film Solar Cells and Solar Cell Testing, Volume II Proceedings of the Fourth Photovoltaic Specialists Conference

Stem cells: Research tools and clinical treatments

Thin film solar cells and solar cell testing - photovoltaic cells, radiation damage to cadmium sulfide solar cells, and airplane testing of solar cells

1964-01-01

94

British Library Electronic Table of Contents (United Kingdom)

Abstract The term -stem cell- most commonly refers to embryonic stem cells, particularly in the lay media; however, it also describes other cell types. A stem cell represents a cell of multi-lineage potential with the ability for self-renewal. It is now clear that the plasticity and immortality of a given stem cell will depend on what type of stem cell it is, whether an embryonic stem cell, a fetal-placental stem cell or an adult stem cell. Stem cells offer great promise as cell-based therapies for the future. With evolving technology, much of the socio-political debate regarding stem cells can now be avoided.

2011-01-01

95

cDNA cloning and sequence analysis of preproendothelin-1 (PPET-1) from salmon, Oncorhynchus keta.

Long-Lasting Inhibitory Effects of Fetal Liver Mesenchymal Stem Cells on T-Lymphocyte Proliferation

The presence of endothelin (ET)-like immunoreactivity and the cardiovascular effects of mammalian ET-1 in fish have been reported. To identify ET-related peptides in fish, we screened the cDNA library of the salmon (Oncorhynchus keta) stomach by means of rapid amplification of cDNA ends, and we cloned cDNAs encoding an ET-related peptide. The salmon ET-related sequence of 21 amino acids is identical to the trout ET-1 peptide recently purified from kidney specimens of Oncorhynchus mykiss. The deduced amino acid sequence of salmon pre-proET-1 (PPET-1) comprises 244 amino acids, including a putative signal sequence and mature ET-1, as well as big ET-1 and ET-1-like sequences. This precursor, the first reported PPET-1 sequence for Salmoniformes, Teleostei, has low homology with the sequences of human, mouse, frog (Xenopus laevis), and zebrafish (Danio rerio) PPET-1 (26%, 29%, 24%, and 39%, respectively). PMID:16740985

2006-06-01

96

Surface modification and characterization for dispersion stability of inorganic nanometer-scaled particles in liquid media

Human bone marrow mesenchymal stem cells (BM-MSC) are multipotent progenitor cells that have transient immunomodulatory properties on Natural Killer (NK) cells, Dendritic Cells (DC), and T cells. This...Full Text Available

97

Studies on application of radiation and radioisotopes -The application of irradiation techniques for food preservation and process improvement-

Inorganic nanoparticles are indispensable for science and technology as materials, pigments and cosmetics products. Improving the dispersion stability of nanoparticles in various liquids is essential for those applications. In this review, we discuss why it is difficult to control the stability of nanoparticles in liquids. We also overview the role of surface interaction between nanoparticles in their dispersion and characterization, e.g. by colloid probe atomic force microscopy (CP-AFM). Two types of surface modification concepts, post-synthesis and in situ modification, were investigated in many previous studies. Here, we focus on post-synthesis modification using adsorption of various kinds of polymer dispersants and surfactants on the particle surface, as well as surface chemical reactions of silane coupling agents. We discuss CP-AFM as a technique to analyze the surface interaction between nanoparticles and the effect of surface modification on the ...

2010-08-01

98

Optical effects of fine-particle carbon on urban atmospheres

The project was designed to solve the infra structural problem required for commercialization of food irradiation. In improvement of physical properties of corn starch, gamma irradiation was effective for increasing glucose productivity and for substituting traditional modified starches (acid modified starch, oxidized starch). In immobilization of microorganisms, the mass production method of natural red pigment was developed by using immobilized mold pellets. In Korean medicinal plants, 10 kGy gamma irradiation was effective for improving sanitary quality and increasing extraction yield. In evaluation of wholesomeness, gamma irradiated red ginseng could be safe on the genotoxic point of view. And also, six items of irradiated foods approved for human consumption from Korea ministry of health and welfare in May 19, 1995. 30 figs, 20 tabs, 54 refs. (Author).

1995-07-01

99

Molecular phylogeography of the microturbellarian Monocelis lineata (Platyhelminthes: Proseriata) in the North-East Atlantic

The effects of fine-particle C, such as diesel soot, on the optical properties of urban haze in the visible wavelength range were explored to determine the dominant effects and to see if simple parameters (such as visual range in green) provide an adequate measure of these effects. It is known that fine-particle C absorbs more strongly in the blue than in the red, so that when it is mixed with a white pigment, the resulting gray can appear somewhat brown. The possibility of a similar effect in urban hazes was investigated, but found not to occur. When the sun is overhead, the near-horizon sky chromaticities caused by mixtures of fine-particle C and non-absorbing aerosol can also be produced by non-absorbing aerosols alone. It is shown that absorbing aerosol will darken the horizon sky, and a simple equation for the radiance of the horizon sky is derived. The effect of absorbing aerosol on the distance at which white and black objects can be seen is calculated.

1986-01-01

100

British Library Electronic Table of Contents (United Kingdom)

Monocelis lineata is a complex of cryptic species (three in the Mediterranean and one in the Atlantic) widespread in midlittoral habitats. Throughout the range, populations with or without an ocular pigmented shield are found. We investigated the genetic structure of the North-East Atlantic populations with the aim of shedding light on their phylogeography and reconstructing possible patterns of recolonization after the Wrmian glaciation. Fourteen samples were investigated using cytochrome c oxidase subunit I (COI) and 13 by inter-simple sequence repeats (ISSRs). COI did not exhibit a clear pattern of decreased genetic diversity along a latitudinal gradient. Populations from Ferrol (Spain), Doolin (Ireland), and Helsingr (Denmark) showed a higher genetic variability, whereas a reduction in...

2011-01-01

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101

Lead uptake and lead loss in the fresh water field crab, Barytelphusa guerini, on exposure to organic and inorganic lead

Curcumin Binding to DNA and RNA

Lead is a heavy metal which is widely used in paint industry, pigments, dyes, electrical components and electronics, plastic chemicals and in various other things. Since some of the lead salts are soluble in water, lead presents a potential threat to aquatic organisms. Studies dealing with invertebrates include those on mortality, growth and lead uptake in Lymnaea palustris and bioaccumulation of heavy metals in oysters and mussels. Little information exists regarding the effect of lead on the fresh water crustaceans. Hence the present investigation has been undertaken to study the uptake and loss of lead on exposure to subtoxic levels or organic and inorganic lead.

1987-07-01

102

British Library Electronic Table of Contents (United Kingdom)

Curcumin, the yellow pigment from the rhizoma of Curcuma longa, is a widely studied phytochemical with a variety of biological activities. The ongoing research and clinical trials have proved that this natural phenolic compound has great and diverse pharmacological potencies. Beside its effective antioxidant, antiinflammatory, and antimicrobial/antiviral properties, curcumin is also considered as a cancer chemopreventive agent. While the antioxidant activity of curcumin is well documented, its interaction with DNA and RNA is not fully investigated. This study was designed to examine the interactions of curcumin with calf thymus DNA and yeast RNA in aqueous solution at physiological conditions, using constant DNA and RNA concentration (6.25?mM) and various curcumin/polynucleotide (phosphate...

2009-01-01

103

A novel volumetric method for quantitation of titanium dioxide in cosmetics

British Library Electronic Table of Contents (United Kingdom)

Nowadays there are many sun-protection cosmetics incorporating organic or inorganic UV filters as active ingredients. Chemically stable inorganic sunscreen agents, usually metal oxides, are widely employed in high-SPF (sun protection factor) products. Titanium dioxide is one of the most frequently used inorganic UV filters. It has been used as a pigment for a long period of cosmetic history. With the development of micronization techniques, it has become possible to incorporate titanium dioxide in sunscreen formulations without the previous whitening effect, and hence its use in cosmetics has become an important research topic. However, there are very few works related to quantitation of titanium dioxide in sunscreen products. In this research, we analysed the amounts of titanium dioxide i...

2007-01-01

104

Targeting vaccines to dendritic cells

Simultaneous recognition and segmentation of cells: application in C.elegans

Udgivelsesdato: 2002-Mar

2002-01-01

105

Adenosquamous cell carcinoma of the gallbladder.

Motivation: Automatic recognition of cell identities is critical for quantitative measurement, targeting and manipulation of cells of model animals at single-cell resolution. It has been...Full Text Available

2011-10-15

106

Spectral absorption properties of colored dissolved organic matter (CDOM) and total suspended matter (TSM) of inland waters

PMID:21290990

2011-01-01

107

Multiple courses of high-dose total skin electron beam therapy in the management of mycosis fungoides

Spectral absorption properties of total suspended matter (TSM) and colored dissolved organic matter (CDOM) are important for the use of the bio-optical model to estimate water quality parameters. This study aims to investigate the variation in the absorption coefficients of TSM and CDOM of inland waters. A total of 92 water samples were collected from Shitoukoumen Reservoir and Songhua Lake in Northeast China, analyzed for TSM and Chl-a, and measured for the absorption coefficient of TSM, CDOM and total pigments using a laboratory spectrophotometer. The absorption coefficient of TSM has been decomposed for phytoplankton and inorganic sediments. The results show that for Shitoukoumen Reservoir, CDOM has strong absorptions with shallow absorption slopes (i.e., the coefficient S in a(?)=a(?0)exp[-S(?- ?0)]) and large absorption at 355 nm; and for Songhua Lake, CDOM follows similar spectral absorption curves but less variation in the S value. The results also show TSM ...

2010-08-01

108

The extracellular calcium sensing receptor is expressed in mouse mesangial cells and modulates cell proliferation.

Purpose: A retrospective analysis was undertaken to determine the indications for, the efficacy of, and the long-term complications of two courses of total skin electron beam therapy for mycosis fungoides. Methods and Materials: A retrospective analysis of 15 patients with the pathologic diagnosis of mycosis fungoides treated in the Department of Radiation Oncology at Stanford University Medical Center between 1968 and 1990 was performed. All patients received two courses of high-dose electron beam therapy to the skin. The mean dose for the total skin treatment for the first course was 32.6 Gy and 23.4 Gy for the second course of treatment. Results: Following the first course of total skin electron beam therapy, 11 of 15 had a complete response, with a mean duration of 11.6 months. All patients received adjuvant therapies between the first and second courses of high-dose total skin electron beam therapy. The mean interval between the first and the second courses of therapy was 41.3 ...

1995-07-30

109

Specific Btk inhibition suppresses B cell? and myeloid cell?mediated arthritis

Full Text Available

2005-10-01

110

Investigation of a Thermal Stress for the Unit Cell of a Solid Oxide Fuel Cell

Abstract not provided

2011-08-29

111

First-generation fuel cell demonstration and commercialization activities

Full Text Available

2011-05-01

112

Applied and fundamental aspects of plant cell, tissue, and organ culture

... electric utilities electrochemistry energy storage fuel cells organizational models

113

Telomeric DNA in normal and leukemic blood cells.

Applied and fundamental aspects of plant cell, tissue, and organ culture

1977-01-01

114

Putative intermediates in the nerve cell differentiation pathway in hydra have properties of multipotent stem cells

We studied telomeric DNA in leukemic cells as well as in normal T cells, B cells, monocytes, polymorphonuclear leukocytes, and bone marrow hematopoietic progenitor cells. No marked differences were...Full Text Available

1995-03-01

115

Solar Cell Radiation Response near the Interface of Different ...

We have investigated the properties of nerve cell precursors in hydra by analyzing the differentiation and proliferation capacity of interstitial cells in the peduncle of Hydra oligactis, which is a region of active nerve cell differentiation. Our results indicate that about 50% of the interstitial cells in the peduncle can grow rapidly and also give rise to nematocyte precursors when transplanted into a gastric environment. If these cells were committed nerve cell precursors, one would not expect them to differentiate into nematocytes nor to proliferate apparently without limit. Therefore we conclude that cycling interstitial cells in peduncles are not intermediates in the nerve cell differentiation pathway but are stem cells. The remaining interstitial cells in the peduncle are ...

1990-12-01

116

Silicon solar cell assembly

... Solar Cell Radiation Respinnse Near the Interface o~f fliffprerv- ... 5 4. CALCUTl-ATED SOLAR CELL RLSPONSE FOR VARIOUS BASE MATERIALS ...

1971-11-01

117

Patterns of proliferation and differentiation of irradiated haemopoietic stem cells cultured on normal 'stromal' cell colonies in vitro

A silicon solar cell assembly comprising a large, thin silicon solar cell bonded to a metal mount for use when there exists a mismatch in the thermal expansivities of the device and the mount.

1979-01-01

118

Oxidation of ethane by an Acremonium species.

Experiments were designed to elucidate whether or not the irradiated bone marrow cells receive any stimulation for the self-replication and differentiation from normal 'stromal' cell colonies in the bone marrow cell culture in vitro. When irradiated or unirradiated bone marrow cells were overlaid on the normal adherent cell colonies, the proliferation of haemopoietic stem cells was supported, the degree of the stimulation depending on the starting cellular concentration. There was, however, no significant changes in the concentration of either CFUs or CFUc regardless of the dose of irradiation on the bone marrow cells overlaid. This was a great contrast to the dose-dependent decrease of CFUs or CFUc within the culture in which both the stem cells and stromal cells were ...

1981-09-01

119

Of Microenvironments and Mammary Stem Cells

Ethane oxidation was studied in ethane-grown resting cells (mycelia) of an Acremonium sp. and in cell-free preparations of such mycelia. From resting cell experiments evidence was found for a pathway...Full Text Available

1976-07-01

120

Laser-Based Fuel Cell Manufacturing for Thermal ...

In most adult tissues there reside pools of stem and progenitor cells inside specialized microenvironments referred to as niches. The niche protects the stem cells from inappropriate expansion...Full Text Available

2007-06-01

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121

Fuel Cell Technologies Equipment on Environmental Expert

... FLUENT PEM fuel cell toolbox is actually composed of many User Defined Functions that are incorporated into the fuel cell model developed by ...

2005-10-12

122

Epithelial cells derived from human embryonic stem cells display p16INK4A senescence, hypermotility, and differentiation properties shared by many P63+ somatic cell types

... Fuel Cell Technologies Equipment on Environmental Expert Find and compare a variety of fuel cell technologies equipment on the world's largest environmental industry portal. View ...

123

Embryonic Retinal Cells and Support to Mature Retinal Neurons

Udgivelsesdato: 2009-Jun

2009-01-01

124

Culture of cells from beagles with bronchioloalveolar carcinoma

Purpose.There is a paucity of neuron replacement studies for retinal ganglion cells. Given the complex phenotype of these neurons, replacement of ganglion cells may be impossible....Full Text Available

2010-04-01

125

Circulating Skeletal Stem Cells

Cell cultures were prepared from lung tumors occurring in beagles following exposure to inhaled plutonium. Morphologic and growth characteristics of two of these cell lines are described.

1977-05-01

126

Cancer cells cause vascular endothelial cell (vEC) retraction via 12(S)HETE secretion; the possible role of cancer cell derived microparticle.

We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from the blood of four mammalian species. These cells phenotypically resemble but are...Full Text Available

2001-05-28

127

Effects of cell concentrations on the survival and repopulation of haemopoietic stem cells in irradiated bone marrow cell culture in vitro

PMID:17103063

2006-11-12

128

Stability of lutein in wholegrain bakery products naturally high in lutein or fortified with free lutein.

Effects of cell concentrations on the survival and repopulation of haemopoietic stem cells after irradiation were studied in the long-term culture of mouse bone marrow cells in vitro. No difference was observed in the survival of the stem cells among cultures in which 0 - 10/sup 7/ cells were re-inoculated on the adherent cell colonies in the culture flask. Stem cells showed a significant proliferation within 1 week and the number of the stem cells exceeded the control in 3 weeks after irradiation in the cultures with less than 10/sup 6/ re-inoculated cells per flask. In contrast, there was a considerable delay in the onset of stem cell proliferation after irradiation in the culture with 10/sup 7/ cells per flask. Based on these results, a possibility that a ...

1981-12-01

129

Rhodopsin photochemistry is vibrationally coherent

Lutein is a yellow pigment found in common foods that promotes the health of eyes and skin and is associated with reduced risk of age-related macular degeneration and cataracts. In the present study, selected high-lutein wheat and corn were milled into wholegrain flours by two mills to improve flour uniformity. The high-lutein and lutein-fortified wholegrain flours were processed into breads, cookies, and muffins to study lutein stability during baking and subsequent storage. Lutein and its isomers were separated, identified, and quantified by LC-UV/vis and LC-MS following extraction with water-saturated 1-butanol. Baking resulted in a significant reduction in all-trans-lutein and the formation of cis-lutein and cis-zeaxanthin isomers. Subsequent storage at ambient temperature had a slight impact on the content of all-trans-lutein. Effects of processing were more pronounced in lutein-fortified products, and the degradation rate of lutein was influenced by ...

2010-09-22

130

Radiation-induced reduction of ditetrazolium salt in aqueous solutions

Visual excitation is initiated by the absorption of a photon by the 11-cis retinal chromophore bound within the pigment called rhodopsin. We have used a variety of vibrational spectroscopies to obtain information about the vibrational nuclear dynamics that lead to this efficient photochemical isomerization. The cis-trans isomerization in rhodopsin is complete in only 200 fs. The extreme speed of this process, which is consistent with the {approximately}50 fs lifetime indicated by the spontaneous emission yield, suggests that the photochemistry involves non-stationary states or vibrational coherence. Recent studies have in fact observed vibrationally coherent oscillations of the ground state photoproduct called bathorhodopsin following impulsive excitation of the rhodopsin reactant. This conclusively demonstrates that the isomerization process in rhodopsin is vibrationally coherent. These observations further suggest that the isomerization quantum yield is directly ...

1995-12-31

131

Lead recycling; Namari no recycle

Color formation in aqueous solutions of the ditetrazolium salt blue tetrazolium (BT{sup 2+}) in the absence or presence of oxygen is a complex radiation chemical reaction. The final stable product is the poorly soluble diformazan violet to blue pigment having a broad spectral absorption band ({lambda}{sub max}=552 nm). The reaction of BT{sup 2+} with the hydrated electron proceeds by rapid reduction of BT{sup 2+} followed by protonation at the nitrogen closest to the unsubstituted phenyl group, via the two intermediate tetrazolinyl radicals shared by the ditetrazole ring nitrogens. The effect of solution pH, N{sub 2}O saturation, and the presence of the reducing agent dextrose are examined. The system serves as a radiochromic sensor and a dosimeter of ionizing radiations. Solutions of 5 mmol l{sup -1} BT{sup 2+} at pH 7.3 serve as dosimeters over an absorbed-dose range of approximately 0.2-6 kGy (dearated, with a range of 1-8 mmol l{sup -1} dextrose) and of about ...

2002-04-01

132

EB-curing of coatings on wood composite boards

In Japan, lead has been widely used for gasoline additives, inorganic chemicals such as pigment, lead pipes/plates, and coating materials of cable. Because of the steady increase in car population and the mounting of environmental concern, lead consumption ratio for lead-acid batteries is tending to increase gradually up to 70% with decreasing the consumption for gasoline additives. This paper describes the recycling of lead-acid batteries in Japan. Since the latter half of FY 1994, the battery manufacturing industry started the new lead recycling system. The recycling ratio of the used lead-acid batteries became 90% in 1996 from 84% in 1994. In near future, it can reach to 95%, the recycling ratio in some European countries. The primary smelting occupies 53%, and the secondary smelting occupies 47%. For the conventional method by secondary smelting makers, reduction smelting and copper removing of lead electrodes are conducted, and lead is recycled as tertiary ...

1997-12-25

133

Detailed industrial hygiene survey, formaldehyde production, E. I. DuPont de Nemours and Co. , Chemicals and Pigment Department, Grasselli Plant, Linden, New Jersey

The industrial radiation processing using low energy electron beam (EB) accelerators lower than 300 keV offers high speed, safe technologies for the chemical conversion of thin layer coatings. Because of the nonselective mode of initiating chain reaction polymerization involving free radicals in synthetic coating layers and suitable substrates, the EB curing of the coatings on woods and papers has particular advantage. Hungary decided to start an up-to-date EB line to process cement-bound (CB) wood chipboards with pigmented acrylic coatings. The CB wood chipboards contain more than 60 % of portland cement and up to 40 % of wood particles. They are produced as large boads of 6 - 16 mm thickness. In their fireproof character and other aspects, they are similar to asbestos-cement boards without containing carcinagenic asbestos, and are stable against moisture and atmospheric influences. EB-cured acrylate coating improved further those properties, and makes them ...

1988-10-01

134

The inhibition of tumor cell intravasation and subsequent metastasis through the regulation of in vivo tumor cell motility by the tetraspanin CD151

A survey to assess the techniques used to control occupational exposure to formaldehyde (50000) and methanol (67561) was conducted at the E. I. DuPont de Nemours and Company (SIC-2869) formaldehyde production unit, Linden, New Jersey, in October 1982. Exposure concentrations were reduced primarily by the use of a process that was completely enclosed except for process sampling, methanol unloading, and formaldehyde discharge points. Local and area exhaust ventilation, and work practices were also used to control exposures. Formaldehyde concentrations in the absorber area were controlled by burning the exit gases in an afterburner and discharging them high above the ground. Analysis of area and breathing zone samples showed methanol and formaldehyde concentrations below the OSHA limits of 200 and 3 parts per million, respectively. The authors conclude that workers are not overexposed to either methanol or formaldehyde on a short or long term basis. Maintenance workers should not be ...

1983-09-01

135

Subcellular Localization of Expansin mRNA in Xylem Cells12

SummaryIn vivo tumor cell migration through integrin-dependent pathways is key to the metastatic behavior of malignant cells. Using quantitative in vivo...Full Text Available

2008-03-01

136

Regulation of asymmetric cell division in the epidermis

Terminal differentiation of many vascular cells involves cell wall changes. Cells first elongate their primary wall, then lay down a lignified secondary wall, which is often followed by digestion of...Full Text Available

2000-06-01

137

RNA recognition by the embryonic cell fate determinant and germline totipotency factor MEX-3

For proper tissue morphogenesis, cell divisions and cell fate decisions must be tightly and coordinately regulated. One elegant way to accomplish this is to couple them with asymmetric cell divisions....Full Text Available

138

RNA expression in a cartilaginous fish cell line reveals ancient 3? noncoding regions highly conserved in vertebrates

Totipotent stem cells have the potential to differentiate into every cell type. Renewal of totipotent stem cells in the germline and cellular differentiation during early embryogenesis rely upon posttranscriptional...Full Text Available

2009-12-01

139

Proteome of human colon cancer stem cells: A comparative analysis

We have established a cartilaginous fish cell line [Squalus acanthias embryo cell line (SAE)], a mesenchymal stem cell line derived from the embryo of an elasmobranch, the spiny dogfish...Full Text Available

2007-01-23

140

Presence of preactivated T cells in hemodialyzed patients: their possible role in altered immunity.

AIM: To isolate and identify the biological characteristics of human colon cancer stem cells (SW1116 cells) and further study their proteome.METHODS: SW1116 cells were isolated and cultured with...Full Text Available

2011-03-14

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141

Parameter Optimization for a Polymer Electrolyte Membrane Fuel Cell Model

Interleukin 2 (IL-2) and B-cell growth factors I and II (BCGF I and BCGF II) are lymphokines produced by T cells that play a major role in T- and B-cell cooperation. Peripheral blood lymphocytes from...Full Text Available

1986-10-01

142

Onset of Quiescence Following p53 Mediated Down-Regulation of H2AX in Normal Cells

Not Available

2010-11-01

143

Nucleoside Drugs Induce Cellular Differentiation by Caspase-Dependent Degradation of Stem Cell Factors

Normal cells, both in vivo and in vitro, become quiescent after serial cell proliferation. During this process, cells can develop immortality with genomic instability,...Full Text Available

144

NOTCH3 expression is induced in mural cells through an autoregulatory loop that requires endothelial-expressed JAGGED1

BackgroundStem cell characteristics are an important feature of human cancer cells and play a major role in the therapy resistance of tumours. Strategies to target cancer stem cells...Full Text Available

145

Muscle satellite cell proliferation and association: new insights from myofiber time-lapse imaging

Endothelial cells and mural cells (smooth muscle cells, pericytes, or fibroblasts) are known to communicate with one another. Their interactions not only serve to support fully functional blood...Full Text Available

2009-02-27

146

Mesenchymal Stem Cells for Craniofacial Tissue Regeneration: Designing Hydrogel Delivery Vehicles

BackgroundAs the resident stem cells of skeletal muscle, satellite cells are activated by extracellular cues associated with local damage. Once activated, satellite cells will re-enter...Full Text Available

147

Knockout of Arabidopsis ACCELERATED-CELL-DEATH11 encoding a sphingosine transfer protein causes activation of programmed cell death and defense

Craniofacial injuries require a variety of different cell types to repopulate areas of bone, cartilage, tendon, and fat. Mesenchymal stem cells (MSCs) provide a multipotent cell source for tissue...Full Text Available

2009-08-01

148

Induced pluripotent stem cells, new tools for drug discovery and new hope for stem cell therapies

We describe the lethal, recessive accelerated-cell-death11 Arabidopsis mutant (acd11). Cell death in acd11 exhibits characteristics of animal apoptosis...Full Text Available

2002-02-15

149

Function of mesenchymal stem cells following loading of gold nanotracers

Somatic cell nuclear transfer or therapeutic cloning has provided great hope for stem cell-based therapies. However therapeutic cloning has been experiencing both ethical and technical difficulties....Full Text Available

2009-01-01

150

Extrafollicular reticulum cells in pathologic lymph nodes.

Background:Stem cells can differentiate into multiple cell types, and therefore can be used for cellular therapies, including tissue repair. However, the participation of stem cells...Full Text Available

2011-01-01

151

Experimental Study of Assembly Clamping Pressure on Performance of PEM Fuel Cells

Full Text Available

1994-02-01

152

Evidence that cell surface heparan sulfate is involved in the high affinity thrombin binding to cultured porcine aortic endothelial cells.

Not Available

2010-12-01

153

Evidence for Diversity in Transcriptional Profiles of Single Hematopoietic Stem Cells

It has been postulated that thrombin binds to endothelial cells through, at least in part, cell surface glycosaminoglycans such as heparan sulfate, which could serve as antithrombin cofactor on the...Full Text Available

1985-04-01

154

Epigenetic changes accompanying human mammary epithelial cell Immortalization

Hematopoietic stem cells replenish all the cells of the blood throughout the lifetime of an animal. Although thousands of stem cells reside in the bone marrow, only a few contribute to blood production...Full Text Available

2006-09-01

155

Electrochemical Performances of Lithium-air Cell with Carbon Materials

No abstract prepared.

2000-10-03

156

Effect of Electromagnetic Fields on Growth of Human Cell Lines

Full Text Available

2010-11-01

157

Cell-density-dependent Changes in the Metabolism of Chloronema Cell Cultures

Full Text Available

2001-10-01

158

Cell Polarity Regulator PARD6B Is Essential for Trophectoderm Formation in the Preimplantation Mouse Embryo1

In the growing chloronema cell suspension cultures of the moss Funaria hygrometrica Hedw., activities of several enzymes have been found to be cell-density-dependent. Cyclic nucleotide...Full Text Available

1979-07-01

159

3 CFR - Guidelines for Human Stem Cell Research

In preimplantation mouse development, the first cell lineages to be established are the trophectoderm (TE) and inner cell mass. TE possesses epithelial features, including apical-basal cell polarity...Full Text Available

2010-09-01

160

Cell-mediated mutagenesis and cell transformation of mammalian cells by chemical carcinogens. [Rats, hamsters

...false Guidelines for Human Stem Cell Research Presidential Documents ...2009 Guidelines for Human Stem Cell Research Memorandum for the Heads...scientifically worthy human stem cell research, including human...

2010-01-01

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161

Basic Information | Fuel Cells &

We have developed a cell-mediated mutagenesis assay in which cells with the appropriate markers for mutagenesis are co-cultivated with either lethally irradiated rodent embryonic cells that can metabolize carcinogenic hydrocarbons or with primary rat liver cells that can metabolize chemicals carcinogenic to the liver. During co-cultivation, the reactive metabolites of the procarcinogen appear to be transmitted to the mutable cells and induce mutations in them. Assays of this type make it possible to demonstrate a relationship between carcinogenic potency of the chemicals and their ability to induce mutations in mammalian cells. In addition, by simultaneously comparing the frequencies of transformation and mutation induced in normal diploid hamster cells by benzo(a)pyrene (BP) and one of its metabolites, it is possible to estimate the genetic ...

1977-01-01

162

Transfection of endothelial cells with EJ-ras oncogene affects cell cycle and the expression of hyaluronic acid, syndecan-4, epimerase and sulfotransferases

...Basic Information | Fuel Cells & Vehicles | US EPA This web page provides basic information on EPA's Fuel Cells & Vehicles web site ...including the chemical composition of fuel cell technology, how it works, descriptions of the various types of fuel cells , their availability and ... background,electrochemical,hydrogen,fuel cell,fuel cell vehicle,fuel reformer,performace,improved fuel economy,increased engine efficiency,lower emissions,zero emissions,availablity,fuel cell types,diagram,Proton ...Exchange Membrane,PEM Basic Information | Fuel Cells & Vehicles | US EPA background,electrochemical,hydrogen,fuel cell,fuel cell vehicle,fuel reformer,performace,improved fuel economy,...

163

Stem Cell Research Policy Lesson Plan

No abstract prepared.

2005-07-01

164

Solar-cell airplane Solair 1

Students get a background on Stem Cell technology and learn to write an effective opinion essay.

2007-09-25

165

Laser-assisted solar cell metallization processing. Quarterly report, December 1984-February 1985

The article describes the Solair 1 solar cell-powered aircraft. Measures to reduce the weight of the craft are cited.

1983-03-01

166

Heavy Metals Effect on Animal Cells

Laser-assisted processing techniques for producing high-quality solar cell metallization patterns are investigated, developed, and characterized. The tasks comprising these investigations are outlined. A new batch of solar cells was processed using the laser decomposition of spun-on silver neodecanoate to metallize cells. Decomposition of silver neodecanoate was carried out at different laser powers on different cells on a given wafer to determine whether this would have any effect on cell performance. Solar cells which were written with laser powers of 5 to 8 watts, gave excellent results with cell efficiencies in the range of 14 to 16%.

1985-04-04

167

International Science & Technology Center (ISTC)

Studies of the Superheavy-metal Effect as a Stress-factor on Protein Biosynthesis and Cytoskeleton Integrity in Animal Cells

168

A NEW FORM OF SOLID STATE SOLAR GENERATOR

Establishment and Maintenance of Human Embryonic Stem Cell Lines on Human Feeder Cells Derived from Uterine Endometrium ...

... nent to the design and construction of metal-semiconductor solar cells, in that both the photovoltage and the efficiency of metal-semiconductor cells ...

1962-01-01

169

Bortezomib induces apoptosis in T lymphoma cells and natural killer lymphoma cells independent of Epstein-Barr virus infection

... and pathogen transmission. Because the ultimate object of stem cell research is cell-based clinical therapy, hES cells should ... 4%) Supported by grants (SC12021 and SC11012) from Stem Cell Research...

170

British Library Electronic Table of Contents (United Kingdom)

Abstract Epstein-Barr virus (EBV), which infects not only B cells, but also T cells and natural killer (NK) cells, is associated with multiple lymphoid malignancies. Recently, the proteasome inhibitor bortezomib was reported to induce apoptosis of EBV-transformed B cells. We evaluated the killing effect of this proteasome inhibitor on EBV-associated T lymphoma cells and NK lymphoma cells. First, we found that bortezomib treatment decreased the viability of multiple T and NK cell lines. No significant difference was observed between EBV-positive and EBV-negative cell lines. The decreased viability in response to bortezomib treatment was abrogated by a pan-caspase inhibitor. The induction of apoptosis was confirmed by flow cytometric assessment of annexin V staining. Additionally, cleavage o...

2011-01-01

171

Convoluted cells as a marker for maternal cell contamination in CVS cultures

Two subpopulations of stem cells for T cell lineage

In order to identify cells of maternal origin in CVS cultures, tissue from 1st trimester abortions were cultivated and the cultures stained in situ for X-chromatin. Convoluted cells and maternal fibroblasts were found to be positive. By chromosome analysis of cultures from 105 diagnostic placenta biopsies, obtained by the transabdominal route, metaphases of maternal origin were found in nine cases. In eight of these cases colonies of convoluted cells were observed. We conclude that convoluted cells are of maternal origin and are a reliable marker for maternal cell contamination in CVS cultures.

1987-01-01

172

Telomerase-immortalized non-malignant human prostate epithelial cells retain the properties of multipotent stem cells

An assay system for the stem cell that colonizes the thymus and differentiates into T cells was developed, and by using this assay system the existence of two subpopulations of stem cells for T cell lineage was clarified. Part-body-shielded and 900-R-irradiated C57BL/6 (H-2b, Thy-1.2) recipient mice, which do not require the transfer of pluripotent stem cells for their survival, were transferred with cells from B10 X Thy-1.1 (H-2b, Thy-1.1) donor mice. The reconstitution of the recipient's thymus lymphocytes was accomplished by stem cells in the donor cells and those spared in the shielded portion of the recipient that competitively colonize the thymus. Thus, the stem cell activity of donor cells can be evaluated by determining the proportion of donor-type (Thy-1.1+) ...

1985-11-01

173

In vitro atrazine-exposure inhibits human natural killer cell lytic granule release

Understanding prostate stem cells may provide insight into the origin of prostate cancer. Primary cells have been cultured from human prostate tissue but they usually survive only 15-20 population doublings before undergoing senescence. We report here that RC-170N/h/clone 7 cells, a clonal cell line from hTERT-immortalized primary non-malignant tissue-derived human prostate epithelial cell line (RC170N/h), retain multipotent stem cell properties. The RC-170N/h/clone 7 cells expressed a human embryonic stem cell marker, Oct-4, and potential prostate epithelial stem cell markers, CD133, integrin #alpha#2#beta#1"h"i and CD44. The RC-170N/h/clone 7 cells proliferated in KGM and Dulbecco's Modified Eagle Medium with 10% fetal bovine serum and 5 #mu#g/ml insulin (DMEM + 10% FBS + Ins.) ...

2008-01-01

174

British Library Electronic Table of Contents (United Kingdom)

The herbicide atrazine is a known immunotoxicant and an inhibitor of human natural killer (NK) cell lytic function. The precise changes in NK cell lytic function following atrazine exposure have not been fully elucidated. The current study identifies the point at which atrazine exerts its affect on the stepwise process of human NK cell-mediated lyses of the K562 target cell line. Using intracellular staining of human peripheral blood lymphocytes, it was determined that a 24-h in vitro exposure to atrazine did not decrease the level of NK cell lytic proteins granzyme A, granzyme B or perforin. Thus, it was hypothesized that atrazine exposure was inhibiting the ability of the NK cells to bind to the target cell and subsequently inhibit the release of lytic protein from the NK cell. To test t...

2007-01-01

175

Immunoselection and clinical use of T regulatory cells in HLA-haploidentical stem cell transplantation

British Library Electronic Table of Contents (United Kingdom)

Introduction: Haploidentical transplantation, with extensive T cell depletion to prevent GvHD, is associated with a high incidence of infection-related deaths. The key challenge is to improve immune recovery with allogeneic donor T cells without triggering GvHD. As T regulatory cells (Tregs) controlled GvHD in pre-clinical studies, the present study evaluated the impact of an infusion of donor CD4/CD25 + Tregs, followed by an inoculum of donor mature T cells (Tcons) and positively immunoselected CD34 + cells in the setting of haploidentical stem cell transplantation. Patients and methods: Twenty-eight patients were enrolled in this study (22 AML; 5 ALL; 1 NHL). All received immunoselected Tregs (CliniMACS, Miltenyi Biotec) followed by positively immunoselected CD34 + cells together with Tc...

2011-01-01

176

The mitogenic activity of human T-cell leukemia virus type I is T-cell associated and requires the CD2/LFA-3 activation pathway.

The presence of a high number of activated T cells in the bloodstream and spontaneous proliferation of peripheral blood mononuclear cells in vitro are striking characteristics of human T-cell leukemia...Full Text Available

1993-06-01

177

Solar cells

In vitro atrazine-exposure inhibits human natural killer cell lytic granule release

The article is the second part of a review dealing with latest developments in the area of solar cell technologies and application. Physical principles, design and efficiency as well as advantages and disadvantages of GaAs- and CdS-solar cells are described. Power generation solar cell systems with voltage converters, combined solar cell/solar collector systems and thermoelectric solar systems are presented in the second part of the article.

1983-04-01

178

Improved Mobilization of the CD34⁺ and CD133⁺ Bone Marrow-Derived Circulating Progenitor Cells by Freshly Isolated Intracoronary Bone Marrow Cell Transplantation in Patients with Ischemic Heart Disease

The herbicide atrazine is a known immunotoxicant and an inhibitor of human natural killer (NK) cell lytic function. The precise changes in NK cell lytic function following atrazine exposure have not been fully elucidated. The current study identifies the point at which atrazine exerts its affect on the stepwise process of human NK cell-mediated lyses of the K562 target cell line. Using intracellular staining of human peripheral blood lymphocytes, it was determined that a 24-h in vitro exposure to atrazine did not decrease the level of NK cell lytic proteins granzyme A, granzyme B or perforin. Thus, it was hypothesized that atrazine exposure was inhibiting the ability of the NK cells to bind to the target cell and subsequently inhibit the release of lytic protein from the NK cell. To test this hypothesis, flow cytometry ...

2007-06-01

179

Identification of a nuclear-localized nuclease from wheat cells undergoing programmed cell death that is able to trigger DNA fragmentation and apoptotic morphology on nuclei from human cells

Cell therapy is a promising novel option for treatment of cardiovascular disease. Because the role of bone marrow-derived circulating progenitor cells (BM-CPCs) after cell therapy is less clear, we...Full Text Available

2011-09-01

180

Expression of alternatively spliced human T-lymphotropic virus type I pX mRNA in infected cell lines and in primary uncultured cells from patients with adult T-cell leukemia/lymphoma and healthy carriers.

PCD (programmed cell death) in plants presents important morphological and biochemical differences compared with apoptosis in animal cells. This raises the question of whether PCD arose independently...Full Text Available

2006-08-01

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181

CD5 Is Dissociated from the B-Cell Receptor in B Cells from Bovine Leukemia Virus-Infected, Persistently Lymphocytotic Cattle: Consequences to B-Cell Receptor-Mediated Apoptosis

Although human T-cell lymphotropic virus type I (HTLV-I) is the etiologic agent of adult T-cell leukemia/lymphoma (ATL), the role of viral gene expression in the progression to and maintenance of the...Full Text Available

1992-04-01

182

Basic study of solid oxide fuel cells. Part 5: investigation of fuel cell materials

Bovine leukemia virus (BLV), a retrovirus related to human T-cell leukemia virus types 1 and 2, can induce persistent nonneoplastic expansion of the CD5⁺ B-cell population, termed...Full Text Available

2001-02-01

183

Wafer and Solar Cell Characterization by GT-PVSCAN6000

Solid Oxide Fuel Cell (SOFC) is expected as a new power generation source. The development of SOFC is being conducted by CRIEPI, and so far several reports of the reults were presented. This report examines materials of SOFC. For the purpose, cells were made using some of typical materials and manufacturing processes, and cell performance tests were carried out.

1991-01-01

184

Uptake and Intracellular Activity of Moxifloxacin in Human Neutrophils and Tissue-Cultured Epithelial Cells

The PVSCAN is an instrument designed to characterize silicon solar cell materials and devices. It performs a host of measurements that yield spatial maps of dislocation density, grain distribution, reflectance, and photoresponses from near-junction and the bulk of a solar cell.

2002-08-01

185

Synovial cells are potent antigen-presenting cells for superantigen, staphylococcal enterotoxin B (SEB).

The penetration by moxifloxacin of human neutrophils (polymorphonuclear leukocytes [PMN]) and tissue-cultured epithelial cells (McCoy cells) was evaluated by a fluorometric assay. At...Full Text Available

1999-01-01

186

Structure of Natural Killer Cell Receptor KLRG1 Bound to E-Cadherin Reveals Basis for MHC-Independent Missing Self Recognition

There is ample evidence suggesting that superantigens may act as a triggering factor in the pathogenesis of rheumatoid arthritis (RA). We investigated whether superantigen could activate T cells in...Full Text Available

1995-03-01

187

Stem Cell Research Policies around the World

SUMMARYThe cytolytic activity of natural killer (NK) cells is regulated by inhibitory receptors that detect the absence of self molecules on target cells. Structural studies of...Full Text Available

2009-07-17

188

Stem Cell Research (Updated July 26, 2002).

The proliferation of stem cell research, conflated with its ethical and moral implications, has led governments to attempt regulation of both the science and funding of stem cells. Due to a diversity...Full Text Available

2009-09-01

189

Squamous Cell Carcinoma in South-Eastern Equatorial Rain Forest in Calabar, Nigeria

Embryonic stem cells have the ability to develop into virtually any cell in the body, and may have the potential to treat medical conditions such as diabetes and Parkinsons disease. On August 9, 2001, President Bush announced that for the first time feder...

2002-01-01

190

Spindle cell carcinoma of head and neck: an immunohistochemical and molecular approach to its pathogenesis

Background: In North America and Europe, 80% of invasive skin cancers are basal cell carcinoma while 20% are squamous cell carcinoma (SCC). In contrast, African studies reveal a preponderance...Full Text Available

191

Shield-verification survey of a large hot cell at the FFTF

BackgroundSpindle cell carcinoma (SpCC) is a rare microscopic type of cancer of the mouth and oropharynx. Although SpCC is thought to arise from squamous cell carcinoma (SCC), it...Full Text Available

2007-05-01

192

SELF-RENEWAL AND DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS AS MEASURED BY Oct4 GENE EXPRESSION: EFFECTS OF LIF, ...

This paper describes a radiation shield verification survey of a large hot cell at the Fast Flux Test Facility (FFTF). The following aspects of the shield test are discussed: description of the FFTF; description of the hot cell; the test procedures; radiation protection, and the test results.

1980-01-01

193

SAMHD1: a new insight into HIV-1 restriction in myeloid cells

... from Dr. Austin Smith of the Institute for Stem Cell Research, University of Edinburgh) was modified from the CGR8 ... to thank Dr. Austin Smith, the Institute for Stem Cell Research, University of Edinbu...

194

Results of Source Emissions Testing. UTC Fuel Cell Model ...

Human myeloid-lineage cells are refractory to HIV-1 infection. The Vpx proteins from HIV-2 and sooty mangabey SIV render these cells permissive to HIV-1 infection through proteasomal degradation of...Full Text Available

195

Regulation of G1 Cell Cycle Progression

... Results of Source Emissions Testing UTC Fuel Cell Model PC25C ... Results of Source Emissions Testing: UTC Fuel Cell Model PC25C ...

2004-09-01

196

Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation

Most genetic changes that promote tumorigenesis involve dysregulation of G1 cell cycle progression. A key regulatory site in G1 is a growth factor–dependent restriction point (R) where cells...Full Text Available

2010-11-01

197

Progress with Nonhuman Primate Embryonic Stem Cells1

Target of rapamycin (TOR) kinases control cell growth through two functionally distinct multiprotein complexes. TOR complex 1 (TORC1) controls temporal cell growth and is sensitive to rapamycin, whereas...Full Text Available

2008-09-23

198

Programmed cell death in castor bean endosperm is associated with the accumulation and release of a cysteine endopeptidase from ricinosomes

... based, disease treatment remains promising, the emphasis for stem cell research from the biomedical research community is clear, and ... diagnosis as a novel source of embryos for stem cell research. Repr...

199

Production of placental alkaline phosphatase (PLAP) and PLAP-like material by epithelial germ cell and non-germ cell tumours in vitro.

The cells of the endosperm of castor bean seeds (Ricinus communis) undergo programmed cell death during germination, after their oil and protein reserves have been mobilized. Nuclear...Full Text Available

1999-11-23

200

Product toxicity and cometabolic competitive inhibition modeling of chloroform and trichloroethylene transformation by methanotrophic resting cells.

Placental and placental-like alkaline phosphatase (PLAP) levels in the culture media of 87 cell lines of neoplastic and 'normal' origin were measured by a conventional immunosorbent enzymatic assay...Full Text Available

1994-02-01

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201

Pressure Probe Technique for Measuring Water Relations of Cells in Higher Plants 1

The rate and capacity for chloroform (CF) and trichloroethylene (TCE) transformation by a mixed methanotrophic culture of resting cells (no exogenous energy source) and formate-fed cells were measured....Full Text Available

1991-04-01

202

Ophthalmic abnormalities in patients with cutaneous T-cell lymphoma.

A new method is described for continuously measuring cell turgor pressure (P), hydraulic conductivity (L_p), and volumetric elastic modulus (ε) in higher plant cells, using a pressure...Full Text Available

1978-02-01

203

Neutral endopeptidase inhibits prostate cancer cell migration by blocking focal adhesion kinase signaling

PURPOSE: To determine the frequency of ophthalmic abnormalities in patients with cutaneous T-cell lymphoma (mycosis fungoides and Sézary syndrome) and T-cell lymphoma involving the skin and...Full Text Available

1998-01-01

204

Method of restoring degraded solar cells

Neutral endopeptidase 24.11 (NEP, CD10) is a cell-surface enzyme expressed by prostatic epithelial cells that cleaves and inactivates neuropeptides implicated in the growth of androgen-independent prostate...Full Text Available

2000-12-01

205

Mechanoreceptor Cells on the Tertiary Pulvini of Mimosa pudica L.

Amorphous silicon solar cells have been shown to have efficiencies which degrade as a result of long exposure to light. Annealing such cells in air at a temperature of about 200.degree. C. for at least 30 minutes restores their efficiency.

1983-01-01

206

Loss of red cell chemokine scavenging promotes transfusion-related lung inflammation

Special red cells were found on the adaxial surface of tertiary pulvini of Mimosa pudica and experiments performed to determine the origin and function of these cells. Using anatomical...Full Text Available

2007-11-01

207

Light-induced Adhesion of Spirogyra Cells to Glass ¹

Red cell transfusions are associated with the development of acute lung injury in the critically ill. Recent evidence suggests that storage induced alterations of the red blood cell (RBC) collectively...Full Text Available

2009-01-29

208

Iron Overload, Hematopoietic Cell Transplantation, and Graft-versus-Host Disease

Adhesion of Spirogyra (tentatively, Spirogyra fluviatilis) cells to glass is described. The cells of an algal filament can adhere to a substrate only when they are...Full Text Available

1977-04-01

209

Identification of the Neoplastically Transformed Cells in Marek's Disease Herpesvirus-Induced Lymphomas: Recognition by the Monoclonal Antibody AV37

Many patients who undergo hematopoietic cell transplantation (HCT) present with anemia and have received red blood cell transfusions before HCT. As a result, iron overload is frequent and appears...Full Text Available

2009-10-01

210

Identification of a stem cell candidate in the normal human prostate gland

Understanding the interactions between herpesviruses and their host cells and also the interactions between neoplastically transformed cells and the host immune system is fundamental to understanding...Full Text Available

2002-07-01

211

Human hair genealogies and stem cell latency

Stem cells of the human prostate gland have not yet been identified utilizing a structural biomarker. We have discovered a new prostatic epithelial cell phenotype-expressing cytokeratin 6a (Ck6a+...Full Text Available

2005-03-01

212

BackgroundStem cells divide to reproduce themselves and produce differentiated progeny. A fundamental problem in human biology has been the inability to measure how often stem cells...Full Text Available

213

Human Cloning

Hepatocyte entry leads to degradation of autoreactive CD8 T cells

... is known as "reproductive cloning." The Link to Stem Cell Research Stem cell research and research cloning are closely linked. Scientists in ... to arrive at a position on cloning and stem cell research. Many nations, including the UK, China, and South ...

214

Glycolipids of human primary testicular germ cell tumours.

Although most self-reactive T cells are eliminated in the thymus, mechanisms to inactivate or control T cells specific for extrathymic antigens are required and exist in the periphery. By investigating...Full Text Available

2011-10-04

215

Germ cell sex determination in mammals

The glycolipid content of human non-seminomatous germ cell tumour cell lines correlates with their differentiation lineage. To analyse whether this reflects the situation in primary tumours, we studied...Full Text Available

1996-07-01

216

Genetic heterogeneity in human T-cell leukemia/lymphoma virus type II.

One of the major decisions that germ cells make during their development is whether to differentiate into oocytes or sperm. In mice, the germ cells’ decision to develop as male or female depends...Full Text Available

2009-04-01

217

Experimental attempt to produce mRNA transfected dendritic cells derived from enriched CD34+ blood progenitor cells

DNA from the peripheral blood mononuclear cells of 17 different individuals infected with human T-cell lymphoma/leukemia virus type II (HTLV-II) was successfully amplified by the polymerase chain reaction...Full Text Available

1993-03-01

218

British Library Electronic Table of Contents (United Kingdom)

It Peripheral blood progenitor enriched CD34+ cells (PBPC) are rather often used as stem cell background in cancer patients following high dose therapy. Keeping in mind that precursor dendritic cells (DCs) originate from haematopoietic progenitor cells, purified CD34+ cells might also serve as starting cells for ex-vivo production of DC. The aim of the present study is to develop a clinical grade procedure for ex-vivo production of DC derived from enriched CD34+ cells. Various concentrations of CD34+ cells were grown in gas-permeable Teflon bags with different serum-free and serum-containing media supplemented with GM-CSF, IL-4, TNF-a, SCF, Flt-3L and INF-a. Serum-free CellGroSCGM medium for 7 days followed by CellGroDC medium in 7 days gave equal results as serum-containing ...

2008-01-01

219

Exceptional sensitivity of testicular germ cell tumour cell lines to the new anti-cancer agent, temozolomide.

Establishment of trophectoderm and inner cell mass lineages in the mouse embryo

Metastatic testicular germ cell tumours are cured in approximately 85% of patients using cisplatin-based combination chemotherapy. Patients who fail to respond have a poor prognosis, and there is a...Full Text Available

1995-05-01

220

Establishment and expression of cellular polarity in fucoid zygotes.

The first cell lineage specification in mouse embryo development is the formation of trophectoderm (TE) and inner cell mass (ICM) of the blastocyst. This article is to review and discuss the...Full Text Available

2009-11-01

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221

Energetic constraints on the creation of cell membrane pores by magnetic particles.

Zygotes of fucoid algae have long been studied as a paradigm for cell polarity. Polarity is established early in the first cell cycle and is then expressed as localized growth and invariant cell division....Full Text Available

1992-06-01

222

Effects of ultrafine particles-induced oxidative stress on Clara cells in allergic lung inflammation

Naturally occurring and contaminant ferromagnetic and ferrimagnetic particles have been found within or near cells, and might allow pulsed magnetic fields to create transient cell membrane opening ("pores")....Full Text Available

1996-08-01

223

Effects of Protons and HZE Particles on Glutamate Transport in Astrocytes, Neurons and Mixed Cultures

BackgroundClara cell protein (CC16), the main secretory product of bronchiolar Clara cells, plays an important protective role in the respiratory tract against oxidative stress and...Full Text Available

224

Effects of Perfluorocarbons on surfactant exocytosis and membrane properties in isolated alveolar type II cells

... ions. MATERIALS AND METHODS Cell Culture Culture of NT2/D1 cells was carried out as described previously (18, 19). NT2 cells were plated at a density of 2. ... ...

225

Characteristics of early- and late-recruited oxytocin bursting cells at the beginning of suckling in rats.

BackgroundPerfluorocarbons (PFC) are used to improve gas exchange in diseased lungs. PFC have been shown to affect various cell types. Thus, effects on alveolar type II (ATII) cells...Full Text Available

2010-01-01

226

Cerebellar cell surface antigens of mouse brain.

1. Paired or single recordings of paraventricular and/or supraoptic oxytocin cells at the beginning of suckling in urethane-anaesthetized rats enabled us to study cell recruitment and compare the characteristics...Full Text Available

1988-05-01

227

Cells navigate with a local-excitation, global-inhibition-biased excitable network

Reaggregated cells from 6- to 8-day-old mouse cerebella have been used to raise antibodies in rabbits. The interaction of these antibodies with cerebellar cell surface components was assessed by cytotoxicity...Full Text Available

1975-10-01

228

Cells involved in the graft-versus host reaction in vitro

Cells have an internal compass that enables them to move along shallow chemical gradients. As amoeboid cells migrate, signaling events such as Ras and PI3K activation occur spontaneously on pseudopodia....Full Text Available

2010-10-05

229

Cell resilience in species lifespans: a link to inflammation?

The cell types involved in the cellular immune response were studied with the GVH in vitro as a test system. Comparison of the activities of cells of different lymphoid organs in the...Full Text Available

1973-07-01

230

CXCL12-Mediated Guidance of Migrating Embryonic Stem Cell-Derived Neural Progenitors Transplanted into the Hippocampus

Species differences in lifespan have been attributed to cellular survival during various stressors, designated here as ‘cell resilience’. In primary fibroblast cultures, cell...Full Text Available

2010-08-01

231

C. elegans as a model for stem cell biology

Stem cell therapies for neurodegenerative disorders require accurate delivery of the transplanted cells to the sites of damage. Numerous studies have established that fluid injections to the hippocampus...Full Text Available

232

Apoptosis of human seminoma cells upon disruption of their microenvironment.

We review the application of C. elegans as a model system to understand key aspects of stem cell biology. The only bona fide stem cells in C. elegans...Full Text Available

2010-05-01

233

An intact microtubule cytoskeleton is not needed for cell cycle progression if the preceding mitosis is of normal duration

One of the main obstacles encountered when trying to culture human seminoma (SE) cells in vitro is massive degeneration of the tumour cells. We investigated whether dissociation of tumour tissue, to...Full Text Available

1996-05-01

234

Activation of peripheral blood mononuclear cells in bronchoalveolar lavage fluid from patients with sarcoidosis: visualisation of single cell activation products.

SummaryFor mammalian somatic cells the importance of microtubule cytoskeleton integrity in interphase cell cycle progression is uncertain. The loss, diminishment, or stabilization...Full Text Available

2007-12-04

235

Abrogation of E-Cadherin-Mediated Cellular Aggregation Allows Proliferation of Pluripotent Mouse Embryonic Stem Cells in Shake Flask Bioreactors

BACKGROUND--Interstitial lung diseases are characterised by the recruitment of mononuclear cells to disease sites where maturation occurs and activation products, including lysozyme (LZM), are released....Full Text Available

1994-11-01

236

A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells

BackgroundA fundamental requirement for the exploitation of embryonic stem (ES) cells in regenerative medicine is the ability to reproducibly derive sufficient numbers of cells of...Full Text Available

237

A homozygous P86S mutation of the human glucagon receptor is associated with hyperglucagonemia, ? cell hyperplasia, and islet cell tumor

Cultured mouse or human embryonic stem (ES) cells provide access to all of the genes required to elaborate the fundamental components and physiological systems of a mammalian cell. Chemical or insertional...Full Text Available

2009-04-01

238

A Practical Approach to Genetic Inducible Fate Mapping: A Visual Guide to Mark and Track Cells In Vivo

ObjectiveThe goal of the study was to investigate the genetic and molecular basis of a novel syndrome of marked hyperglucagonemia and pancreatic α cell hyperplasia...Full Text Available

2009-11-01

239

The advancement of stem cells in radiation medicine

Fate maps are generated by marking and tracking cells in vivo to determine how progenitors contribute to specific structures and cell types in developing and adult tissue. An advance in this...Full Text Available

240

Review of the application of molecular beam epitaxy for high efficiency solar cell research

It may result in acute radiation syndrome after body is exposed to ionizing radiation. The one of long-term effects of irradiation injury is leukemia. The bone marrow cells (BMC) transplantation including stem cells is the only effective therapy for acute radiation syndrome patients. Recently, with the advancement of stem cell research that the stem cells have multipotential and can convert each other, it may supply the new stem source for the irradiation injury patients. At the same time with the further research of radioprotective reagents, the hematopoietic stem cells proliferation after irradiation injury is promoted

2003-02-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

241

Recent progress in a-Si solar cells

In the last two years, rapid progress has been made in the energy conversion efficiencies of GaAs solar cells fabricated from molecular beam epitaxy (MBE) material. The efficiencies of cells fabricated from MBE material are now comparable with those fabricated from metal-organic chemical vapor deposition material, even for cells of dimension 2 cmx4 cm. This paper reviews the progress in MBE cell efficiencies. Also discussed is the role oval defects play in GaAs diode and solar cell performance. (orig.).

1991-05-01

242

Moving toward personalized cell-based interventions for adrenal cortical disorders: Part 2 - Human diseases and tissue engineering

As concern regarding global environmental problems such as the greenhouse effect and acid rain has increased, so too has the demand for commercially viable solar cells as a clean energy source. Interest in amorphous silicon (a-Si) solar cells has been particularly high, due to their low cost. Technological developments in the field of a-Si solar cells are discussed from the viewpoints of fabrication process, materials, and cell structures. Various applications and systems that take advantage of the a-Si solar cell are then introduced. Finally, future prospects are mentioned

1997-04-14

243

British Library Electronic Table of Contents (United Kingdom)

Transdifferentiation of an individual's own cells into functional differentiated cells to replace an organ's lost function would be a personalized approach to therapeutics. In this two part series, we will describe the progress toward establishing functional transdifferentiated adrenal cortical cells. In this article (Part 2), we describe the disorders of the adrenal cortex, therefore establishing why there is the need for personalized cell-based therapy for individuals with these disorders. We then present our pilot studies of cell transdifferentiation toward an adrenal cortical fate using genes described in the first article of this pair (Part 1).

2011-01-01

244

Laser-assisted solar cell metallization processing

High efficiency GaInP/GaAs tandem solar cells

In this contract, Laser-assisted processing techniques for producing high-quality solar cell metallization patterns are being investigated, developed, and characterized. The tasks comprising these investigations are outlined. Four new batches of solar cells were processed, in addition to several test runs on wafers, using the laser decomposition of spun-on silver neodecanoate to metallize cells. Decomposition of silver neodecanoate was carried out at different laser powers on different cells on a given wafer to determine whether this would have any effect on cell performance. A one watt laser power gave an electroplated linewidth of 50 microns , while at 8 watts the line width was 90 microns.

1986-01-01

245

Efficiency of silicon and GaAs concentrator solar cells operated inside integrating cavity receivers

We report on multijunction GaInP/GaAs photovoltaic cells with total-area efficiencies of 29.5% at one-sun concentration and air mass (AM) 1.5 global and 25.7% one-sun, AM0. These values represent the highest efficiencies achieved by any solar cell under these illumination conditions. Three key areas in this technology are identified and discussed: the grid design, front surface passivation of the top cell, and bottom surface passivation of both cells. Aspects of cell design related to its operation under different solar spectra and under concentration are also discussed.

1994-06-30

246

tfrsc fa so - NASA Technical Report Server (NTRS)

The theory for the general case of solar cells operating inside integrating cavity receivers is established. This is applied to the particular case of different configurations of silicon and GaAs cells. The results of the analysis show that a composite system of silicon and GaAs cells manufactured using relatively simple technology could reach an efficiency of 34%. The optimal configuration is that in which the GaAs cells are placed in the directly illuminated area of the receiver and the silicon cells are placed in the indirectly illuminated area of the receiver. (orig.).

1991-06-01

247

Yttrium Y 90 Ibritumomab Tiuxetan, Fludarabine, Radiation Therapy, and Donor Stem Cell Transplant in Treating Patients With Relapsed or Refractory Non-Hodgkin's Lymphoma

pletely new PW fuel cell model had to be developed for CINDA. Several ...... following linear equation, which is used in the SINDA fuel cell model: ...

248

Variation of Mesenchymal Cells in Polylactic Acid Scaffold in an Osteochondral Repair Model

B-cell Chronic Lymphocytic Leukemia; Nodal Marginal Zone B-cell Lymphoma; Recurrent Adult Burkitt Lymphoma; Recurrent Adult Diffuse Large Cell Lymphoma; Recurrent Adult Diffuse Mixed Cell Lymphoma; Recurrent Adult Diffuse Small Cleaved Cell Lymphoma; Recurrent Adult Grade III Lymphomatoid Granulomatosis; Recurrent Adult Immunoblastic Large Cell Lymphoma; Recurrent Adult Lymphoblastic Lymphoma; Recurrent Grade 1 Follicular Lymphoma; Recurrent Grade 2 Follicular Lymphoma; Recurrent Grade 3 Follicular Lymphoma; Recurrent Mantle Cell Lymphoma; Recurrent Marginal Zone Lymphoma; Recurrent Small Lymphocytic Lymphoma; Splenic Marginal Zone Lymphoma; Waldenstrom Macroglobulinemia

2010-10-12

249

Unmasking Stem/Progenitor Cell Properties in Differentiated ...

ObjectiveTo achieve osteochondral regeneration utilizing transplantation of cartilage-lineage cells and adequate scaffolds, it is essential to characterize the behavior of transplanted...Full Text Available

2009-12-01

250

Towards a Synthetic Chloroplast

... In addition to changes in BrdU, we also observed transient changes in p63 gene expression in the myoepithelial/stem cell layer. ...

2007-08-01

251

The manufacture of cadmium sulfide thin film solar cells at the rates of 1 kilowatt per month and 5 kilowatts per month

BackgroundThe evolution of eukaryotic cells is widely agreed to have proceeded through a series of endosymbiotic events between larger cells and proteobacteria or cyanobacteria,...Full Text Available

252

The formation and degradation of Ti-Ag and Ti-Pd-Ag solar cell contacts

Manufacture of cadmium sulfide thin film solar cells

1964-01-01

253

The alpha-gamma lead cell system in the post-irradiation laboratory of Metallurgy Division, AERE, Harwell

Ti-Ag and Ti-Pd-Ag solar cell contacts structure and degradation dependence on high temperature and humidity environmental exposure

1970-01-01

254

The Structure of Plant Cell Walls

... alpha particles beta particles gamma radiation lead hot cells bricks shielding

255

TARDEC Brief to OnPoint Technologies

The molecular structure, chemical properties, and biological function of the xyloglucan polysaccharide isolated from cell walls of suspension-cultured sycamore (Acer pseudoplatanus)...Full Text Available

1973-01-01

256

Strategies to optimize the outcome of children given T-cell depleted HLA-haploidentical hematopoietic stem cell transplantation

... Program: Quallion Matrix Design (small cells) - Investigate the feasibly of a hybrid battery matrix composed of small D-sized cells for use in HEVs ...

2007-02-28

257

British Library Electronic Table of Contents (United Kingdom)

The most advanced frontier of allogeneic hematopoietic stem cell transplantation (allo-HSCT) is represented by the use of an HLA-partially matched relative as donor. In this type of transplantation, donor-derived natural killer (NK) cells, which are alloreactive towardtoward recipient cells, significantly contribute to the eradication of leukemia blasts. Alloreactive NK cells may also kill host dendritic cells and T lymphocytes, thus preventing graft-versus-host disease and graft rejection, respectively. Sophisticated strategies of adoptive infusion of T-cell lines/clones specific for the most life-threatening pathogens (namely cytomegalovirus, Epstein-Barr virus, Aspergillus and Adenovirus) have been envisaged, and successfully tested in a few pilot trials, to protect the recipient in the...

2011-01-01

258

Stochastic gene expression and its consequences

Gene expression is a fundamentally stochastic process, with randomness in transcription and translation leading to significant cell-to-cell variations in mRNA and protein levels. This variation...Full Text Available

2008-10-17

259

Stickiness to Glass

Role of mast cells in the pathogenesis of hypersensitivity pneumonitis.

Conditions were found in which Chlamydomonas reinhardi exhibits a circadian alteration of its cell surface, measured as ability to stick to glass. Under these same conditions the cells...Full Text Available

1979-06-01

260

Role of Mast Cells in Early and Delayed Radiation Injury in Rat Intestine

To examine the possibility that mast cells have a central role in the pathogenesis of hypersensitivity pneumonitis, 20 patients with this disease were studied with the aim of seeking evidence for mast...Full Text Available

1987-08-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

261

Regulatory T cells in human disease and their potential for therapeutic manipulation

... mast cell staining; ref. 16). The severity of structural radiation injury was assessed using a histopathological radiation injury score ... ...

262

Optical Spectroscopy for Noninvasive Monitoring of Stem Cell Differentiation

Regulatory T cells are proposed to play a central role in the maintenance of immunological tolerance in the periphery, and studies in many animal models demonstrate their capacity to inhibit inflammatory...Full Text Available

2006-05-01

263

On the spontaneous emergence of cell polarity

There is a requirement for a noninvasive technique to monitor stem cell differentiation. Several candidates based on optical spectroscopy are discussed in this review: Fourier transform infrared (FTIR)...Full Text Available

2010-01-01

264

Neural Tissues from the Implanted Stem Cells

Diverse cell polarity networks require positive feedback for locally amplifying distributions of signalling molecules at the plasma membrane1. Additional...Full Text Available

2008-08-14

265

International Science & Technology Center (ISTC)

Morphological, Electrophysiological and Behavioral Investigations of the Nervous Tissue Developed from the Embryonic Matrix Zone Cells of the Dorsolateral Walls of Lateral Ventricles, Implanted into the Lesioned Regions of the Adult Rat's Brain

266

NASA - Pico-Satellite Solar Cell Experiment (PSSC)

Modeling of batteries and fuel cells; Proceedings of the Symposium, Phoenix, AZ, Oct. 13-19, 1991

Jun 10, 2011 ... The PSSC is a picosatellite designed to test the space environment by providing a testbed to gather data on new solar cell technologies.

267

Misfolded Proteins and Retinal Dystrophies

The present volume on modeling of batteries and fuel cells discusses the significance of the effectiveness factor for flooded porous electrodes, active pore distribution spectroscopy for characterizing porous battery electrodes, the agglomerate model for porous electrodes, and dynamic-performance measurements of battery cells for electric vehicles and other applications. Attention is given to mathematical modeling of a primary zinc/air battery, mathematical modeling of Grace Li-TiS2 cells, modeling of electrocrystallization processes in battery systems, and rotating disk electrode studies in molten Li/K carbonate eutectic. Topics addressed include the variability of nickel oxide cathode dissolution in molten carbonate fuel cells, water transport properties of fuel cell ionomers, modeling water content effects in polymer electrolyte fuel cells, and computer ...

1991-01-01

268

Laser-assisted solar cell metallization processing - NASA ...

Many mutations associated with retinal degeneration lead to the production of misfolded proteins by cells of the retina. Emerging evidence suggests that these abnormal proteins cause cell death...Full Text Available

2010-01-01

269

Large scintillation cells for high sensitivity radon concentration measurements

Oct 15, 1985 ... Title: Laser-assisted solar cell metallization processing. No Digital Version Available: Go to Tips on Ordering ...

270

Large scintillation cells for high sensitivity radon concentration measurements

Methods for improving the sensitivity of scintillation cells for radon concentration measurements were studied with emphasis on improving light collection efficiency. This allows the length and hence the volume of the cell to be increased. Variables studied were choice of scintillator material, its method of application and thickness, length of cell, cell material, type and configuration of reflectors, choice of photomultipliers, and factors affecting background. Response from various areas of the cell surface was studied with an alpha source and with radon filling. Coating the window with phosphor was found to be counter-productive. The optimum results obtained were with the inside of the cell (other than the window) covered with a thick layer of ZnS(Ag), or with a thick layer of reflective material coated with a thin layer of phosphor. With it, a 10 cm ...

1983-07-01

271

Isolation and differentiation of chondrocytic cells derived from human embryonic stem cells using dlk1/FA1 as a novel surface marker

Methods for improving the sensitivity of scintillation cells for radon concentration measurements were studied with emphasis on improving light collection efficiency. This allows the length and hence the volume of the cell to be increased. Variables studied were choice of scintillator material, its method of application and thickness, length of cell, cell material, type and configuration of reflectors, choice of photomultipliers, and factors affecting background. Response from various areas of the cell surface was studied with an alphy source and with radon filling. Coating the window with phosphor was found to be counter-productive. The optimum results obtained were with the inside of the cell (other than the window) covered with a thick layer of ZnS(Ag), or with a thick layer of reflective material coated with a thin layer of phosphor. With it, a 10 cm ...

272

Immunohistochemical Analysis of Sarcoid Granulomas

Udgivelsesdato: 2009-Dec

2009-01-01

273

Hydrolysis of cis- and trans-Epoxyeicosatrienoic Acids by Rat Red Blood Cells

Proliferating cells have been immunophenotypically characterized in lymph node and bronchoalveolar lavage (BAL) samples obtained from patients with active and inactive sarcoidosis with the cell-cycle-related...Full Text Available

1988-05-01

274

Hormonal Control of Cell Proliferation Requires PASTICCINO Genes

Erythrocytes serve as reservoirs for cis- and trans-epoxyeicosatrienoic acids (EETs). Incubation of rat red blood cells (RBCs) with cis- and...Full Text Available

2008-07-01

275

High-Temperature Solar Cell Development - GLTRS - NASA

PASTICCINO (PAS) genes are required for coordinated cell division and differentiation during plant development. In loss-of-function pas mutants,...Full Text Available

2003-07-01

276

General Disclaimer One or more of the Following Statements may ...

High-Temperature Solar Cell Development. Geoffrey A. Landis,. NASA John Glenn Research Center. 21000 Brookpark Road, Cleveland, OH 44135. Danielle ...

277

Expression of cell proliferation and apoptosis biomarkers in pterygia and normal conjunctiva

1 The Elemental Fuel Cell Model. The cell reactions are also illustrated in. Figure 1. Hydrogen is oxidized at the anode. Oxy- ...

278

Durability of Membrane Electrode Assemblies (MEAs) in PEM Fuel ...

PurposeTo analyze the expression of apoptosis and cell proliferation molecules in pterygium tissues of Chinese patients.MethodsThirty-three pterygia...Full Text Available

279

Dietary Lipids, Cells Adhesion and Breast Cancer Metastasis

Proton exchange membrane (PEM) fuel cells are energy sources that have the ... for H2 /02 PEM fuel cells because their catalysts have properties suitable for 0 ...

280

Development of optical coatings for cos thin film solar cells third quarterly report, jun. 1 - aug. 1, 1965

... Accession Number : ADA396364. Title : Dietary Lipids, Cells Adhesion and Breast Cancer Metastasis. Descriptive Note : Annual rept. ...

2000-10-01

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281

Detection of genetic mutations in repetitive DNA sequences in somatic human cells after low-dose irradiation

Sputtering of glass coatings on cadmium sulfide thin film solar cells

1965-01-01

282

Continuous human cell lines and method of making same

... 3-89701-147-6 0949-2224 245 p. animal cells dna genetic radiation effects

1998-03-01

283

Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo(a)pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors. 2 tabs.

1985-07-01

284

Continuity and change

Cell proliferation and chemical carcinogenesis: symposium overview.

... with the loosening of Bush-era restrictions on stem cell research, a move that was widely hailed by the ... The first two explore the contentious debate over stem cell research. Using a series of intervie...

285

CD44 Occupancy Prevents Macrophage Multinucleation

Cancer, by definition, is a proliferative disease. The fundamental scientific issue explored at the international symposium "Cell Proliferation and Chemical Carcinogenesis" was the impact of chemically...Full Text Available

1993-12-01

286

Analysis of heterogeneous cell populations: A density-based modeling and identification framework

Cells of the mononuclear phagocyte lineage have the capability to adhere to and fuse with each other and to differentiate into osteoclasts and giant cells. To investigate the macrophage adhesion/fusion...Full Text Available

1998-11-02

287

British Library Electronic Table of Contents (United Kingdom)

Abstract: In many biological processes heterogeneity within clonal cell populations is an important issue. One of the most striking examples is a population of cancer cells in which after a common, identical death signal some cells die whereas others survive. The reason for this heterogeneity is intrinsic and extrinsic noise. In this paper we present a mechanistic multi-scale modeling framework for cell populations, in which the dynamics of every individual cell is captured by a parameter dependent stochastic differential equation (SDE). Heterogeneity among individual cells is accounted for by differences in parameter values, modeling extrinsic influences. Based on the statistical properties of the extrinsic noise and the SDE model for the individual cell, a partial differential equation (...

2011-01-01

288

A Theoretical Solid Oxide Fuel Cell Model for - The NASA Glenn ...

2004 Office of Fossil Energy Fuel Cell Program Annual Report

May 31, 2011 ... A Theoretical Solid Oxide Fuel Cell Model for System Controls and Stability Design AUTHOR(S): Kopasakis, George; Brinson, Thomas; Credle, ...

289

20 Watt-Hour Per Pound Regenerative Fuel Cell

Annual report of fuel cell projects sponsored by Department of Energy, National Energy Technology Laboratory.

2004-11-01

290

Committed T lymphocyte stem cells of rats. Characterization by surface W3/13 antigen and radiosensitivity

... for evaluation of the electrochemiral performance of the materials and components used in EOS Rechargeable Fuel Cell Model RHO-24AH-Mod ...

1972-03-01

291

A sample preparation for quantitative determination of magnesium in individual lymphocytes by electron probe X-ray microanalysis.

The existence of stem cells committed to the T lymphoid lineage was deduced from studying how rat T and B stem cells differ in their expression of membrane W3/13 antigen and in their susceptibility in vivo to gamma irradiation. Stem cell activity of rat bone marrow and fetal liver was measured in long-term radiation chimeras using B and T cell alloantigenic surface markers to identify the progeny of donor cells. Monoclonal mouse anti-rat thymocyte antibody W3/13 labeled approximately 40% of fetal liver cells and 60-70% of young rat bone marrow cells (40% brightly, 25% dimly). Bright, dim, and negative cells were separated on a fluorescence-activated cell sorter. All B and T lymphoid stem cells in fetal liver were W3/13 bright, as were B lymphoid stem cells in ...

1981-01-01

292

Some thoughts on stem cells and carcinogenesis. The thyroid gland

We present a sample preparation method for measuring magnesium in individual whole lymphocytes by electron probe X-ray microanalysis. We use Burkitt's lymphoma cells in culture as the test sample and compare X-ray microanalysis of individual cells with atomic absorption analysis of pooled cell populations. We determine the magnesium peak-to-local continuum X-ray intensity ratio by electron probe X-ray microanalysis and calculate a mean cell magnesium concentration of 39 +/- 19 mmol/kg dry weight from analysis of 100 cells. We determine a mean cell magnesium concentration of 34 +/- 4 mmol/kg dry weight by atomic absorption analysis of pooled cells in three cell cultures. The mean cell magnesium concentrations determined by the two methods are not significantly different. We find a 10% coefficient of ...

1986-01-01

293

Role of limited cell replicative capacity in pathological age change. A review

The purpose of this review is to consider the hypothesis that cancer frequently originates from stem cells. Using the spleen transplantation assay where stem cells were transplanted in the spleen of mice lethally irradiated by ionizing radiation, the author undertook a study aimed at defining the risk of radiogenic cancer per susceptible cells with use of rat radiogenic mammary and thyroid cancers because of the high incidences of these cancers in a-bomb survivors. Measured were the number of cancer-susceptible cells initially present in the tissue, the number of such cells that survived at a given dose and the number of cancers that developed per surviving cell. Thyroid cell differentiation and proliferation in rats transplanted with thyroid cells were enhanced by thyroidectomy and low iodine diet. Further, the ...

2000-07-01

294

Gap-junctional communication of bone marrow stromal cells is resistant to irradiation in vitro

Physiological functions are carried out by differentiated cells, with finite lifespans, which age and need to be replaced. In young individuals, tissue functions are sustained at optimal levels because cellular dysfunction and cell loss are balanced by the emergence of newly differentiated cells as stem cells and their partially differentiated descendants replicate. However, with the passage of time the mitotic rates of these cells diminish. Eventually, replications occur too infrequently to offset the loss. It is at this point that the tissue begins to show structural changes and declining function which, as they become pervasive, are identified as ageing. In this paper the theory is set forth that: (1) Diminishing mitotic activity in older tissues results from limited stem cell replicative capacity. (2) All stem cells, regardless of ...

1982-01-01

295

Gap-junctional communication of bone marrow stromal cells is resistant to irradiation in vitro

Bone marrow is one of the most radiosensitive organs. Irradiation causes a marked decrease in the total number of hematopoietic cells in the bone marrow. The reticular meshwork structure of marrow stromal cells, however, is relatively resistant to irradiation. Unimpaired stromal cell structure has been thought to be a prerequisite for the repopulation of hematopoietic cells during recovery from the effects of irradiation. The reticular framework is maintained by cell adhesion apparatuses such as gap junctions. The in vitro radiobiologic survival values of a cloned stromal cell line, H-1/A, were studied (n = 1.8, D0 = 138 cGy). Radiation doses of up to 4000 cGy had no detectable effects on the production of colony-stimulating factor 1. H-1/A cells communicate with each other via gap junctions as determined by the sensitive dye-transfer ...

1990-10-01

296

Electric-field-induced deformation of biological cells

Bone marrow is one of the most radiosensitive organs. Irradiation causes a marked decrease in the total number of hematopoietic cells in the bone marrow. The reticular meshwork structure of marrow stromal cells, however, is relatively resistant to irradiation. Unimpaired stromal cell structure has been thought to be a prerequisite for the repopulation of hematopoietic cells during recovery from the effects of irradiation. The reticular framework is maintained by cell adhesion apparatuses such as gap junctions. The in vitro radiobiologic survival values of a cloned stromal cell line, H-1/A, were studied (n = 1.8, D0 = 138 cGy). Radiation doses of up to 4000 cGy had no detectable effects on the production of colony-stimulating factor 1. H-1/A cells communicate with each other via gap junctions as determined by the sensitive dye-transfer ...

297

The Future of Induced Pluripotent Stem Cells for Cardiac Therapy and Drug Development.

The cell is modeled as an ellipsoid with shell and the surface stress distribution calculated by means of the Maxwell Stress Tensor. The cell membrane is treated as incompressible material having both bending and shear energies of deformation. Employing the principle of virtual work, cell volume (and hence shape) is computed as a function of the external field strength and orientation with respect to the field. The following results were obtained: (1) The surface stresses that develop or the cell membrane are distributed having both normal and tangential components that act together to produce a rounding of the cell. (2) If the tangential component of the stress is ignored, then higher field strengths are needed to produce similar deformation (shape change) of cells. (3) The threshold value of the applied field, i.e, the field strength at which the volume ...

1989-01-01

298

Physical, biochemical and physiological effects of ultraviolet radiation on Brassica napus and Phaseolus vulgaris

The field of stem cell research was revolutionized with the advent of induced pluripotent stem cells. By reprogramming somatic cells to pluripotent stem cells, most ethical concerns associated with the use of embryonic stem cells are overcome, such that many hopes from the stem cell field now seem a step closer to reality. Several methods and cell sources have been described to create induced pluripotent stem cells and we discuss their characteristics in terms of feasibility and efficiency. From these cells, cardiac progenitors and cardiomyocytes can be derived by several protocols and most recent advances as well as remaining limitations are being discussed. However in the short time period this technology has been around, evidence emerges that induced pluripotent stem cells may ...

2011-09-15

299

In order to follow some of the changes induced by ultraviolet-B (UV-B, 280-320 nm) radiation in Phaseolus vulgaris and Brassica napus, experiments were designed to localize sites of changes in leaves and to correlate some of the physiological and biochemical changes with penetration of UV-B radiation. B.napus was exposed to 8.9 kJ m"-"2 day"-"1 biologically effective UV-B radiation (UV-B_B_E). The penetration of UV-B radiation into the leaf was followed using a quartz fibre optic microprobe. Monochromatic radiation at 310 nm was decreased by ca 50 and 34% in the adaxial and abaxial epidermis, respectively, in plants not exposed to UV-B, whereas the radiation was decreased by ca 70 and 42%, respectively, in the same region in UV-treated plants. Polychromatic radiation showed a wavelength dependent change mainly for the collimated radiation. The results correlated with the distribution of phenolic compounds analysed from 40 #mu#m paradermal leaf sections. The first adaxial section ...

1991-11-05

300

Intelligent Extruder

Contribution of calcium ions and hydrogen ions to the signal transduction chain in phytochrome-mediated spore germination. [Onoclea sensibilis L

''Intelligent Extruder'' described in this report is a software system and associated support services for monitoring and control of compounding extruders to improve material quality, reduce waste and energy use, with minimal addition of new sensors or changes to the factory floor system components. Emphasis is on process improvements to the mixing, melting and de-volatilization of base resins, fillers, pigments, fire retardants and other additives in the :finishing'' stage of high value added engineering polymer materials. While GE Plastics materials were used for experimental studies throughout the program, the concepts and principles are broadly applicable to other manufacturers materials. The project involved a joint collaboration among GE Global Research, GE Industrial Systems and Coperion Werner & Pleiderer, USA, a major manufacturer of compounding equipment. Scope of the program included development ...

2003-04-24

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

301

Sex and seasonal differences in the rate of cell proliferation in the dentate gyrus of adult wild meadow voles.

Red light stimulates germination in the spores of Onoclea sensibilis L. Phytochrome is confirmed to be the photoreceptor pigment in the germination response by demonstrating red-far-red photoreversibility. External Ca/sup 2 +/ is required for this response with a threshold at a submicromolar concentration. Red light stimulates an increase in the total concentration of intracellular calcium in the spores as determined by atomic absorption spectroscopy. Subsequent exposure to far-red light inhibits the red light-induced increase in intracellular calcium. The majority of the increase occurs 5 minutes after the onset of irradiation. The calcium-antagonist, La/sup 3 +/ inhibits both germination and the red light-induced increase in intracellular calcium. Using /sup 31/P-nuclear magnetic resonance spectroscopy, the author tested the hypothesis that a sustained increase in intracellular pH contributes to the signal transduction chain. He never detected a red light-induced ...

1985-01-01

302

[Intraspecies antagonism of Sh. flexneri in an HEp-2 cell line model].

In order to study the neurobiological basis of seasonal changes in hippocampal structure and function, the rate of cell proliferation was examined in male and female wild meadow voles captured during different seasons. We found that the number of [3H]thymidine-labeled cells varied across the seasons and across sex in the meadow vole. Non-breeding female meadow voles had a higher rate of cell proliferation and cell death than males captured during either season or breeding females. These seasonal changes in the female meadow vole were associated with both fluctuating levels of adrenal steroids and gonadal steroids. Estradiol level was highly correlated with both the number of [3H]thymidine-labeled cells and the number of pyknotic cells in female meadow voles, with high levels of estradiol being associated with low levels of cell proliferation ...

1999-03-01

303

Turgor Regulation in Osmotically Stressed Arabidopsis Epidermal Root Cells. Direct Support for the Role of Inorganic Ion Uptake as Revealed by Concurrent Flux and Cell Turgor Measurements1

The authors describe an effect of suppression of invasion of the guinea pig eye conjunctiva and the HEp-2 epithelial cells by virulent Sh. flexneri bacilli, with a simultaneous administration of the same dose of avirulent shigella mutants, genetically connected with them. The data of morphological study and experiments with 3H-glucose labeled shigellae carried out on the cell species model indicated that the bacterial competition for the specific sites for absorption on the epithelial cells underlay the observed phenomenon. PMID:331774

1977-06-01

304

The human melanocyte: A model system to study the complexity of cellular aging and transformation in non-fibroblastic cells

Hyperosmotic stress is known to significantly enhance net uptake of inorganic ions into plant cells. Direct evidence for cell turgor recovery via such a mechanism, however, is still lacking. In the...Full Text Available

2002-05-01

305

The degradation mechanism of Ti-Pd-Ag solar cell contacts by an accelerated electrochemical testing technique

No abstract prepared.

2002-05-23

306

The AMOS cell - An improved metal-semiconductor solar cell

No Abstract Available

1984-01-01

307

Testing of solar cells for communication satellites

A new fabrication process is being developed which significantly improves the efficiency of metal-semiconductor solar cells. The resultant effect, a marked increase in the open-circuit voltage, is produced by the addition of an interfacial layer oxide on the semiconductor. Cells using gold on n-type gallium arsenide have been made in small areas (0.17 sq cm) with conversion efficiencies of 15% in terrestrial sunlight.

1975-01-01

308

Stimulation of porcine bone marrow stromal cells by hyaluronan, dexamethasone and rhBMP-2

... cadmium sulfides communications electrons performance testing physical

309

Simon A. Levin's Passion for Ecology

Udgivelsesdato: 2004-Oct

2004-01-01

310

Signet ring cell carcinomas of gastric mucosa in hypocatalasemic mice treated with X-ray or chemicals

... prominent example today, perhaps, is the issue of stem cell research, but there is a whole list of issues ... ...

311

S179D prolactin diminishes the effects of UV light on epidermal gamma delta T cells

Short communication.

312

Round Cell batteries in Class 1E applications

Epidermal gamma delta T cells (γδ T) and Langerhans cells (LC) are immune cells altered by exposure to ultraviolet radiation (UVB), a powerful stressor resulting in immune suppression....Full Text Available

2008-01-02

313

Reproductive Toxicity Assessment on 2-Bromopropane using Spematogenesis Stage Classification and Sertoli Cell Indices

The AT and T Round Cell has recently become a viable alternative to the conventional, rectangular, lead acid storage batteries in Class 1E applications at US nuclear generating stations. This paper discusses the qualification of the Round Cell for nuclear generating stations, its principal design attributes, the types of Class 1E applications most suited for the Round Cell, and industry experience related to its installation and operation.

1996-04-09

314

Regulatory T cells inhibit stable contacts between CD4⁺ T cells and dendritic cells in vivo

Full Text Available

2001-12-01

315

Regulation of cloned, Ca2+-activated K+ channels by cell volume changes.

Regulatory T (T reg) cells exert powerful down-modulatory effects on immune responses, but it is not known how they act in vivo. Using intravital two-photon laser scanning microscopy we determined that,...Full Text Available

2006-03-20

316

Radiation Protection of the Organism (Selected Chapters)

Udgivelsesdato: 2002-May

2002-01-01

317

Proteomic Functional Characterization of Bovine Stromal Vascular Cells from Omental, Subcutaneous and Intramuscular Adipose Depots

... biologically important compounds is sharply lowered with passage of the solutions to the cell an organ, where their sensitivity ...

1970-12-11

318

Partial retraction. Detection of an infectious retrovirus, XMRV, in blood cells of patients with chronic fatigue syndrome.

Full Text Available

2011-01-01

319

Optimized chondrogenesis of ATCD5 cells through sequential regulation of oxygen conditions

PMID:21998366

2011-10-14

320

On the effects of boron and phosphorus primary impurities in p-type silicon material for solar cells

Udgivelsesdato: MAR

2006-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

321

Morphine postconditioning attenuates icam-1 expression on endothelial cells.

Not Available

1979-01-01

322

Microfluidic systems and methods for transport and lysis of cells and analysis of cell lysate

Full Text Available

2011-02-01

323

Micro-Fabricated Thin-Film Fuel Cells for Portable Power Requirements

Microfluidic systems and methods are disclosed which are adapted to transport and lyse cellular components of a test sample for analysis. The disclosed microfluidic systems and methods, which employ an electric field to rupture the cell membrane, cause unusually rapid lysis, thereby minimizing continued cellular activity and resulting in greater accuracy of analysis of cell processes.

2008-09-02

324

Mechanisms of the elimination of low dose hyper-radiosensitivity in T-47D cells by low dose-rate priming

No abstract prepared.

2002-03-21

325

MODIFIED SILK POWDER HAVING EXCELLENT CELL GROWING PROPERTY, METHOD FOR PRODUCING THE SAME AND USE THEREOF

Udgivelsesdato: Dec

2009-01-01

326

J-STORE (Japan)

Full Text Available

2005-02-16

327

Laser-Sintered {TiO}_{2} Films for Dye Solar Cell Fabrication: An Electrical, Morphological, and Electron Lifetime Investigation

Intracellular Calcium Concentration in the Glutamate-induced Cytotoxicity in PCl2 Cell

Not Available

2011-09-01

328

Internalization of cholera toxin by different endocytic mechanisms

Full Text Available

2002-12-01

329

Interaction of an immunodominant epitope with Ia molecules in T-cell activation

Cell biology, clathrin, endocytosis, dynamin, caveolae, cholera toxin

2001-01-01

330

Impairing Effects of Acute Glucose Overload on Calcium Homeostasis in Vascular Endothelial Cells

Udgivelsesdato: 1988-Jul

1988-01-01

331

Illuminating Cell Biology

Full Text Available

1998-12-01

332

Identifying Contributing Degradation Phenomena in PEM Fuel Cell Membrane Electrode Assemblies Via Electron Microscopy

NASA's Ames Research Center awarded Ciencia, Inc., a Small Business Innovation Research contract to

2002-01-01

333

Human breast cancer cell lines contain stem-like cells that self-renew, give rise to phenotypically diverse progeny and survive chemotherapy

No abstract prepared.

2006-10-01

334

Human Mammary Luminal Epithelial Cells Contain Progenitors to Myoepithelial Cells

IntroductionThe phenotypic and functional differences between cells that initiate human breast tumors (cancer stem cells) and those that comprise the tumor bulk are difficult to...Full Text Available

2008-01-01

335

G2 checkpoint siRNA screen in irradiated cancer cells: validation of candidate positive hits

The origin of the epithelial and myoepithelial cells in the human breast has not been delineated. In this study we have addressed whether luminal epithelial cells and myoepithelial cells are vertically connected, i.e., whether one is the precursor for the other. We used a primary culture assay allowing preservation of basic phenotypic traits of luminal epithelial and myoepithelial cells in culture. The two cell types were then separated immunomagnetically using antibodies directed against lineage-specific cell surface antigens into at best 100% purity. The cellular identity was ascertained by cytochemistry, immunoblotting, and 2-D gel electrophoresis. Luminal epithelial cells were identified by strong expression of cytokeratins 18 and 19 while myoepithelial cells were recognized by expression of vimentin and ...

1999-02-01

336

Norwegian Open Research Archives (NORA)

Full Text Available

337

Effect of Extracelluar Matrix on Cell-Surface Interactions and Growth of Cardiomyocyte(HL-1)

Distribution of Calretinin-immunoreactive Unipolar Brush Cells in Ataxic Mutant Pogo Mice Cerebellum

Full Text Available

2010-01-01

338

Distinct roles for dystroglycan, beta1 integrin and perlecan in cell surface laminin organization.

Full Text Available

2005-08-01

339

Different telomere-length dynamics at the inner cell mass versus established embryonic stem (ES) cells

Udgivelsesdato: 2001-Mar

2001-01-01

340

Cytosolic Calcium Alteration and Cell Injury by Silica in Rat Hepatocytes

Murine embryonic stem (ES) cells have unusually long telomeres, much longer than those in embryonic tissues. Here we address whether hyper-long telomeres are a natural property of pluripotent stem cells,...Full Text Available

2011-09-13

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

341

Crosslinked sulfonated poly (bis-A)-sulfones as proton exchange membrane for PEM fuel cell application

Full Text Available

1998-12-01

342

Comparison of Nitrogen Removal Between Reed and Cattail Wetland Cells in a Treatment Pond System

Not Available

2010-02-01

343

Comparison of Conditions for Cell Death-Inducing Agents Using a High Throughput-Compatible Nuclear Staining Assay

Full Text Available

2004-12-01

344

Full Text Available

2008-09-01

345

Cell culture systems to study glial transformation

The state of chromatin during the cell cycle was examined using synchronized cultures of CHO hamster cells. Results support Mazia's dynamic chromosome cycle model and indicate that DNA-interactive chemotherapeutic agents elicit different types of kinetic responses in treated cells, suggesting a degree of specificity of interaction between various alkylating and intercalating agents and the genome. Effects of sarkosyl crystals, heparin, and chemotherapeutic agents, neocarzinostatin and adriamycin, on chromation are discussed. (HLW)

1976-01-01

346

Cell Cycle-dependent Expression of Chitin Synthase Genes in Aspergillus nidulans

The transformation of two different types of glial cells has been studied using an in vivo-/in vitro model and a complete in vitro model. The purpose of the study and to define in vitro model systems is to study the the neoplastic transformation of pure populations of glial cells. Data are presented to demonstrate that the transformed cells are glial and tumorigenic. (ACR)

1980-01-01

347

Capsaicin induced apoptosis and the enhanced anticancer effect of anticancer drugs in cancer cells

Full Text Available

2001-03-01

348

Antiproliferation Effects of Germinated-Korean Rough Rice Extract on Human Cancer Cells

Full Text Available

2008-03-01

349

Amplified B Lymphocyte CD40 Signaling Drives Regulatory B10 Cell Expansion in Mice

Full Text Available

2010-03-01

350

Action of the venom of Apis mellifera and gamma radiation on mammalian cells

BackgroundAberrant CD40 ligand (CD154) expression occurs on both T cells and B cells in human lupus patients, which is suggested to enhance B cell CD40 signaling and play a role...Full Text Available

351

A study on improving the surface morphology of recycled wafer forsolar cells using micro_blaster

Published in summary form only.

1988-07-01

352

A Scheme for Reuse of Residual Energy in a Multi-cell Battery System

Full Text Available

2010-07-01

353

A Monoclonal Antibody That Specifically Binds Chitosan In Vitro and In Situ on Fungal Cell Walls

Full Text Available

2009-11-01

354

Zinc-air battery/fuel cell

Full Text Available

2010-08-01

355

The effects of high-intensity pulsed electromagnetic field on proliferation and differentiation of neural stem cells of neonatal rats in vitro

The zinc-air battery/fuel cell is an old technology invented one hundred years ago. However, there is renewed interest in this technology in response to the growing need for clean energy technology. The zinc-air battery/fuel cell is more attractive than similar technologies because its characteristics include high power density, safe operation and storage, and low cost. Zinc-air battery/fuel cells can be made in milliwatts to mega watts to accommodate different applications. The zinc-air battery/fuel cell has four major designs, namely primary, mechanically rechargeable, continuous feed and electrically rechargeable zinc-air battery/fuel cells. Among the different designs, the most common is the air cathode. There are 3 generations of catalysts used in the air cathodes. This paper discussed the different designs of the zinc-air battery/fuel cell, and more ...

2010-07-01

356

British Library Electronic Table of Contents (United Kingdom)

Summary The effects of high-intensity pulsed electromagnetic stimulation (HIPEMS) on proliferation and differentiation of neonatal rat neural stem cells in vitro were investigated. Neural stem cells derived from neonatal rats were exposed to 0.1 Hz, 0.5-10 Tesla (T) [8 groups of B-I, respectively], 5 stimuli of HIPEMF. The sham exposure controls were correspondingly established. Inverted phase contrast microscope was used to observe the cultured cells, MTT assay to detect the viability of the cells as expressed by absorbance (A) value, and flow cytometry to measure differentiation of neural stem cells. The results showed that A values of neural stem cells in both 3.0 T and 4.0 T groups were significantly higher than the other groups 24 to 168 h post HPEMS, indicating a strong promotion of ...

2009-01-01

357

Role of the diffuse layer in acidic and alkaline fuel cells

British Library Electronic Table of Contents (United Kingdom)

A numerical model is developed to study electrolyte dependent kinetics in fuel cells. The model is based on the Poisson-Nernst-Planck (PNP) and generalized-Frumkin-Butler-Volmer (gFBV) equations, and is used to understand how the diffuse layer and ionic transport play a role in the performance difference between acidic and alkaline systems. The laminar flow fuel cell (LFFC) is used as the model fuel cell architecture to allow for the appropriate comparison of equivalent acidic and alkaline systems. We study the overall cell performance and individual electrode polarizations of acidic and alkaline fuel cells for both balanced and unbalanced electrode kinetics as well as in the presence of transport limitations. The results predict cell behavior based on electrolyte composition that strongly...

2011-01-01

358

Resveratrol causes COX-2- and p53-dependent apoptosis in head and neck squamous cell cancer cells

British Library Electronic Table of Contents (United Kingdom)

Cyclooxygenase-2 (COX-2) content is increased in many types of tumor cells. We have investigated the mechanism by which resveratrol, a stilbene that is pro-apoptotic in many tumor cell lines, causes apoptosis in human head and neck squamous cell carcinoma UMSCC-22B cells by a mechanism involving cellular COX-2. UMSCC-22B cells treated with resveratrol for 24 h, with or without selected inhibitors, were examined: (1) for the presence of nuclear activated ERK1/2, p53 and COX-2, (2) for evidence of apoptosis, and (3) by chromatin immunoprecipitation to demonstrate p53 binding to the p21 promoter. Stilbene-induced apoptosis was concentration-dependent, and associated with ERK1/2 activation, serine-15 p53 phosphorylation and nuclear accumulation of these proteins. These effects were blocked by ...

2008-01-01

359

Polymer Fuel Cells Challenge

PKH26 as a fluorescent label for live human umbilical mesenchymal stem cells

...Polymer Fuel Cells Challenge Publications News Events Login Register Search Content type All Web pages Case studies Publications News Video Home ...Buildings Carbon capture & storage Combined heat & power Electricity transmission & distribution Energy storage Fuel cells Geothermal Hydroelectric Hydrogen Industry Lighting Marine Metering Nuclear Solar Transport Wind ...Home Emerging technologies Current focus areas Polymer Fuel Cell Challenge Polymer Fuel Cells Challenge The objective of the Polymer Fuel Cells Challenge is to develop,...prove and commercialise novel polymer fuel cell technologies that have the potential to deliver a step-change in overall system cost. What are ...

360

British Library Electronic Table of Contents (United Kingdom)

To determine whether PKH26 labeling affects the morphologies, phenotypes, proliferation, and secretion abilities of human umbilical mesenchymal stromal cells (HUMSCs) were investigated. Isolated HUMSCs were labeled with PKH26, and cell morphology was observed under microscope. Cell cycle, apoptotic cell death, expression of PKH26, and the proliferation rate were evaluated. Additionally, fluorescence intensity of PKH26 labeling at different passage times was quantified. There were no detectable differences in cell morphology, cell growth, and proliferation rate after PKH26 labeling. In addition, fluorescence intensity of PKH26 labeling was gradually reduced with increase of the passage times. The PKH26 labeling disappeared after passage six times. In summary, PKH26 labeling is a safe and ef...

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

361

Molecular pathology of tumor-initiating cells: Lessons from Philadelphia chromosome-positive leukemia

British Library Electronic Table of Contents (United Kingdom)

Recent improvements in cell purification and transplantation techniques have contributed to the identification of cell populations known as tumor-initiating cells (TIC). This discovery has led to the -cancer stem cell hierarchy- concept, which holds that tumors are organized as a hierarchy of malignant tissues sustained by such TIC. However, this concept remains controversial. In this review, we examine recent advances in cancer stem cell research that have been generated from studies of Philadelphia (Ph) chromosome-positive leukemia. The abnormal Ph chromosome, which arises from a translocation creating the BCR-ABL1 fusion gene, is most commonly associated with chronic myelogenous leukemia (CML) and precursor B cell acute lymphoblastic leukemia (B-ALL). Examination of the pathophysiology ...

2011-01-01

362

Molecular marks for epigenetic identification of developmental and cancer stem cells

British Library Electronic Table of Contents (United Kingdom)

Epigenetic regulations of genes by reversible methylation of DNA (at the carbon-5 of cytosine) and numerous reversible modifications of histones play important roles in normal physiology and development, and epigenetic deregulations are associated with developmental disorders and various disease states, including cancer. Stem cells have the capacity to self-renew indefinitely. Similar to stem cells, some malignant cells have the capacity to divide indefinitely and are referred to as cancer stem cells. In recent times, direct correlation between epigenetic modifications and reprogramming of stem cell and cancer stem cell is emerging. Major discoveries were made with investigations on reprogramming gene products, also known as master regulators of totipotency and inducer of pluoripotency, na...

2011-01-01

363

Lysis of Escherichia coli cells by lysozyme: Discrimination between adsorption and enzyme action

British Library Electronic Table of Contents (United Kingdom)

The key factors of enzymatic lysis of cells are the interaction between the enzyme and the cell - catalytic and non-catalytic adsorption of enzyme on cell surface. Here, the studies of lysis of intact Escherichia coli cells by chicken egg white lysozyme were performed. It was found that the ionic strength has a dual effect onto the system. On the one hand, the desorption constant of the enzyme increases with the increase of the solution ionic strength, which results in a better enzyme performance. On the other hand, due to the higher osmosis, the cell lysis rate decreases with the increasing of ionic strength of the system. It was found that pH 8.6 and 30mM NaCl are optimal conditions for lysis of E. coli cells by lysozyme.

2011-01-01

364

Long-Term Follow-Up of Patients with Breast Cancer Transplanted with Autologous Ex Vivo Expanded Peripheral Blood Progenitor Cells

British Library Electronic Table of Contents (United Kingdom)

Ex vivo expanded peripheral blood progenitor cells (PBPC) have been shown to provide rapid neutrophil engraftment, and in some patients, to eliminate neutropenia after transplantation to support high-dose chemotherapy. However, the effect of expansion culture on stem cell content and potential loss of stem cells caused by induction of differentiation remains a concern. We have transplanted 21 patients with breast cancer with expanded autologous PBPC, with 11 patients receiving expanded PBPC as their sole hematopoietic cell source. In these studies, the CD34+ cells were selected and cultured for 10 days in defined media containing 100 ng/mL each of recombinant human stem cell factor (rhSCF), recombinant human granulocyte colony stimulating factor (rhG-CSF), and recombinant human megakaryocy...

2006-01-01

365

Laser beam apparatus and method for analyzing solar cells

Involvement of the Tpl-2/cot oncogene in MMTV tumorigenesis.

A laser beam apparatus and method for analyzing, inter alia, the current versus voltage curve at the point of illumination on a solar cell and the open circuit voltage of a solar cell. The apparatus incorporates a lock-in amplifier, and a laser beam light chopper which permits the measurement of the AC current of the solar cell at an applied DC voltage at the position on the solar cell where the cell is illuminated and a feedback scheme which permits the direct scanning measurements of the open circuit voltage. The accuracy of the measurement is a function of the intensity and wavelength of the laser light with respect to the intensity and wavelength distribution of sunlight and the percentage the dark current is at the open circuit voltage to the short circuit current of the solar cell.

1980-01-01

366

Importance of serum source for the in vitro replicative senescence of human bone marrow derived mesenchymal stem cells

We report for the first time a relationship between the Tpl-2/cot oncogene and Mouse Mammary Tumor Virus (MMTV) associated transformation of mammary gland cells. A sub-genomic library generated from a primary mammary gland tumor yielded a novel MMTV integration site which disrupted the Tpl-2/cot proto-oncogene between exons 7 and 8. Comparison of a cell line derived from normal mammary gland (comma-D) and a cell line established from an MMTV induced mammary tumor (GR) demonstrated similar rearrangements within Tpl-2/cot for the GR cells but not in the comma-D cells. These rearrangements in the cell line were accompanied by an increase in the level of Tpl-2/cot specific mRNA. This data suggests that Tpl-2/cot expression may be important in epithelial cell transformation or tumor progression. PMID:8934549

1996-11-01

367

British Library Electronic Table of Contents (United Kingdom)

Abstract Human mesenchymal stem cells (hMSCs) may be used for therapeutic applications. Culture conditions such as the serum source may impact on cell quality and the onset of replicative senescence. We have examined the effect of culturing hMSCs in autologous serum (AS) versus fetal bovine serum (FBS) on factors involved in in vitro replicative senescence. hMSCs from four donors were cultured in 10% FBS or 10% AS until they reached senescence. Cells were harvested at early passage and near senescence to study factors known to be involved in cellular senescence. The number of population doublings till senescence was similar for cells cultured in FBS, but varied greatly for hMSCs cultured in AS. FBS cells accumulated in S phase of cell cycle. This could not be explained by increased express...

2011-01-01

368

Establishment of functional primary cultures of heart cells from the clam Ruditapes decussatus

British Library Electronic Table of Contents (United Kingdom)

Heart cells from the clam Ruditapes decussatus were routinely cultured with a high level of reproducibility in sea water based medium. Three cell types attached to the plastic after 2?days and could be maintained in vitro for at least 1?month: epithelial-like cells, round cells and fibroblastic cells. Fibroblastic cells were identified as functional cardiomyocytes due to their spontaneous beating, their ultrastructural characteristics and their reactivity with antibodies against sarcomeric ?-actinin, sarcomeric tropomyosin, myosin and troponin T-C. Patch clamp measurements allowed the identification of ionic currents characteristic of cardiomyocytes: a delayed potassium current (I K?slow) strongly suppressed (95%) by tetraethylammonium (1?mM), a fast inactivating potassium current (I K?fas...

2011-01-01

369

Developing defined culture systems for human pluripotent stem cells

British Library Electronic Table of Contents (United Kingdom)

Human pluripotent stem cells hold promising potential in many therapeutics applications including regenerative medicine and drug discovery. Over the past three decades, embryonic stem cell research has illustrated that embryonic stem cells possess two important and distinct properties: the ability to continuously self-renew and the ability to differentiate into all specialized cell types. In this article, we will discuss the continuing evolution of human pluripotent stem cell culture by examining requirements needed for the maintenance of self-renewal in vitro. We will also elaborate on the future direction of the field toward generating a robust and completely defined culture system, which has brought forth collaborations amongst biologists and engineers. As human pluripotent stem cell re...

2011-01-01

370

Cytokine-induced impairment of short-chain fatty acid oxidation and viability in human colonic epithelial cells

Cancer-cell-specific cytotoxicity of non-oxidized iron elements in iron core-gold shell NPs

Pro-inflammatory cytokines may directly influence the viability and metabolic function of colonic epithelial cells (CEC) as an early event in the development of inflammatory bowel disease. We report here that TNF-alpha+IFN-gamma induced a synergistic, concentration-dependent decline in butyrate oxidation, an essential energy supply, in HT-29 and DLD-1 cells. TNF-alpha+IFN-gamma induced a parallel profound decline in cell viability in HT-29 cells, but not in DLD-1 cells, where impairment of butyrate oxidation seemed to precede later occurrence of cell damage. TNF-alpha+INF-gamma induced CEC damage was independent on NO formation and involved the IFN-gamma signalling pathway as well as induction of apoptosis. If cytokines have similar effects in vivo, these may lead to energy deficiency and thus contribute to CEC damage and disturbance of the epithelial integrity.

2000-01-01

371

British Library Electronic Table of Contents (United Kingdom)

Gold-coated iron nanoparticles (NPs) selectively and significantly (P <0.0001) inhibit proliferation of oral- and colorectal-cancer cells in vitro at doses as low as 5 mg/mL, but have little adverse effect on normal healthy control cells. The particle treatment caused delay in cell-cycle progression, especially in the S-phase. There was no significant difference in the NP uptake between cancer and control cells, and cytotoxicity resulted primarily from the iron core, before oxidation, rather than from the Fe ions released from the core. In contrast with magnetic NPs that usually serve as drug carriers, diagnostic probes or hyperthermia media, the iron, before oxidation, in the NPs selectively suppressed cancer cell growth and left healthy control cells unaffected in vitro and in vivo. This...

2011-01-01

372

Bufalin induces autophagy-mediated cell death in human colon cancer cells through reactive oxygen species generation and JNK activation

British Library Electronic Table of Contents (United Kingdom)

Colorectal cancer is the second most common cause of cancer death in the world and about half of the patients with colorectal cancer require adjuvant therapy after surgical resection. Therefore, the eradication of cancer cells via chemotherapy constitutes a viable approach to treating patients with colorectal cancer. In this study, the effects of bufalin isolated from a traditional Chinese medicine were evaluated and characterized in HT-29 and Caco-2 human colon cancer cells. Contrary to its well-documented apoptosis-promoting activity in other cancer cells, bufalin did not cause caspase-dependent cell death in colon cancer cells, as indicated by the absence of significant early apoptosis as well as poly(ADP-ribose) polymerase and caspase-3 cleavage. Instead, bufalin activated an autophagy...

2011-01-01

373

Biphasic Low-Grade Nasopharyngeal Papillary Adenocarcinoma with a Prominent Spindle Cell Component: Report of a Case Localized to the Posterior Nasal Septum

British Library Electronic Table of Contents (United Kingdom)

A case (female, 39?years of) of thyroid-like nasopharyngeal low-grade papillary adenocarcinoma with a significant spindle cell component is presented. The tumor was located on the posterior nasal septum. The spindle cells displayed nuclear features very much similar to the epithelial component and the two cell types merged imperceptibly. Immunohistochemically, the neoplastic cells (including the spindle cell component) were strongly and diffusely positive for TTF-1, cytokeratins (AE1-3), cytokeratin 19 and vimentin. C-kit immunohistochemistry showed diffuse mild to moderate membranous positivity with focal areas displaying moderate to strong immunoreactivity. EMA was strongly positive in the epithelial component with membranous and cytoplasmic reactivity whereas the spindle cell component ...

2011-01-01

374

Applications and benefits of the FFTF IEM cell training facility

The influence of electromagnetic field irradiated by high-voltage transmission lines on properties of cells.

The Interim Examination and Maintenance (IEM) Cell is located within the Fast Flux Test Facility (FFTF) Reactor Containment Building. This cell is a complex vertical hot cell whose purpose is to process reactor experiments and to perform maintenance on reactor and refueling components. Because access to this very complex cell is limited, a mock-up called the IEM Training Facility (IEMTF) has been developed. The IEMTF provides the IEM cell with many valuable benefits. Four of these benefits are: (1) development of alternate processing methods; (2) hands-on evaluation of equipment problems; (3) a ready source of verified parts, and (4) training facilities for IEM Cell technicians.

1982-05-01

375

Laser-assisted solar cell-metallization processing. Quarterly report, June 13-September 12, 1984

The influence of low-frequency electromagnetic field irradiating by high-voltage transmission lines on signal transduction of cell in spleen cells of the rates have been studied by molecular-biology techniques. The spleen cells are extracted from skilled rates, which are exposed in the electromagnetic field of high-voltage transmission lines with 4000 V/m and 0.09-0.1 G about 400 days. The quantity or level of phosphorylation of signal transducer and activator of transcription (STAT3) in JAK-STAT signal transduction pathway of spleen cells, which are stimulated and unstimulated by IL-2, respectively, are detected by the immunoblotting and immunobiochemistry. The results show that the expression of phospho-STAT3 in spleen cell stimulated by IL-2 differ not from that in the unstimulated cell. The former is significantly large than the latter. This shows that ...

2005-01-01

376

Extreme sensitivity of some intestinal crypt cells to X and #gamma# irradiation

High-quality solar cells have been fabricated by utilizing localized argon-ion laser decomposition of silver neodecanoate spun onto diffused silicon substrates and subsequent electroplating. Early adhesion problems during electroplating have been carefully studied and finally solved using a novel coating procedure. The laser-metallized solar cells have been characterized using lighted and dark current-voltage measurements and compared with baseline cells metallized using standard photolithographic procedures. Non-AR-coated cell efficiencies ranging from 10 to over 11% have been obtained for the laser-metallized cells, comparable with the best baseline cells. The laser-metallized cells have 30 to 40% lower series resistance than the unsintered baseline cells, indicating that in-situ sintering takes place during laser ...

1984-10-30

377

Cyclin-Dependent Kinase Inhibitor p16 and p21 Expression, and Cell Cycle Change in Human Lens Epithelial Cell Line SRA 01/04 following Contact Inhibition in Normal Culture

Reference is made to Cheng and Leblond (Amer. J. Anat.; 141:537 (1974)) who used tritiated thymidine to kill cells synthesising DNA in the crypts of the small intestine. Amongst their findings was that very low doses caused measurable cell killing and that the killing was not random throughout the crypt but occurred selectively at the crypt base where stem cells are presumably located. It is here reported that the presence of hypersensitive cells at the base of the crypts can be demonstrated after whole-body X- or gamma-irradiation, and the time sequence for the production and loss of the killed cells is described together with their dose-response relationship. The studies were conducted on mice. The data were expressed as surviving rather than killed cells, and it was established that the crypt are amongst the most sensitive of mammalian ...

1977-10-01

378

British Library Electronic Table of Contents (United Kingdom)

Abstract Purpose: To describe the pattern of expression of the cyclin-dependent kinase inhibitors (CDKIs) p16, p21 and p27, and the cell cycle in SRA 01/04 cells relative to contact inhibition. Methods: SRA 01/04 cells were grown to overconfluence under normal conditions. At various phases of the cell growth, cells were assayed by flow cytometry and Western blotting for the expression of CDKIs. Results: Expression of p16 was detected from early logarithmic growth to stationary phases, during which the number of cells in G0/G1 increased from 46 to 69%. Expression of p21 was detected only during the overgrowth phase, when 60% of the cells were in G0/G1. Expression of p27 was not observed in SRA 01/04 cells. Conclusions: p16 expression was likely mediated by G0/G1 arrest to induce contact inh...

2011-01-01

379

Conceptual design of a sodium sulfur cell for US electric-van batteries

State-of-the-art in photovoltaic research and application (except for use in concentrators)

A conceptual design of an advanced sodium/sulfur cell for US electric-van applications has been completed. The important design factors included specific physical and electrical requirements, service life, manufacturability, thermal management, and safety. The capacity of this cell is approximately the same as that for the ``PB`` cell being developed by Silent Power Limited (10 Ah). The new cell offers a 50% improvement in energy capacity and nearly a 100% improvement in peak power over the existing PB cells. A battery constructed with such cells would significantly exceed the USABC`s mid-term performance specifications. In addition, a similar cell and battery design effort was completed for an advanced passenger car application. A battery using the van cell would have nearly 3 times the energy compared to lead-acid ...

1993-05-01

380

Lead oxides-lithium cells

A review is given of the state-of-the-art of single and polycrystalline solar cells, which includes a short theoretical review, laboratory achievements, and production methods. The Si single and polycrystalline cell and the amorphous Si cell are described, including material preparation, crystal and sheet growth, and cell and panel production. Promising second generation thin film solar cells including GaAs, CdS(CuInSe/sub 2/), and CdTe are briefly described. Economical aspects are discussed.

1987-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

381

Labeled cells as research, diagnostic and therapeutic tools

The possibility of using lead and lead-bismuth mixed oxides as positive active materials in organic electrolyte lithium cells with a working voltage similar to those of silver zinc cells has been considered. Button cells of SR 44 size have been developed as a test vehicle and studied under various conditions of discharge rate and storage. This paper describes the performance characteristics obtained under these conditions and suggests in conclusion the possible replacement of silver zinc cells by such systems for a large range of low-rate applications on the basis of cost effectiveness.

1979-01-01

382

Experimental Proof Of Principle For The Link-Up Of A Solid Oxide Fuel Cell With A Counter-Current Wood Gasifier

Scientists at JPL have developed chemical and biological techniques using microspheres filled with drugs, electron-opaque metals, or radioactive, fluorescent, magnetic or electrically charged materials to label specific groups of cells. Synthetic polymeric microspheres are coupled with specific antibodies to form reagents called immunomicrospheres, which can seek out and attach themselves to any specific group of cells. These cell-labeling techniques, therefore, open new avenues not only to the basic study of cells but also to the diagnosis and treatment of many diseases, including cancer.

1983-09-01

383

Dipyrone-induced changes in DNA repair and other cell membrane associated processes in Escherichia coli

The combination of wood gasification and high temperature fuel cells like solid oxide fuel cells (SOFC) is an interesting approach to achieve high electrical efficiencies (40%) in small scale combined heat and power plants. This combination is called 'Biomass - Integrated Gasification Fuel Cell System' (B-IGFC). The main challenge is the adjustment of the three main components gasification, gas cleaning and fuel cell. In this technology, the first proof of principle by combining a gasifier with a SOFC in a laboratory scale for more than 100 hours was achieved. During this period, no deactivation of the system was found. (author)

2005-03-01

384

Dipyrone-induced changes in DNA repair and other cell membrane associated processes in Escherichia coli

The analgesic, dipyrone (1,phenyl-2,3-dimethyl-5-pyrazolone-4-methylamino methane sulphonate sodium), at 20 mM concentration, inhibited the rejoining of single-strand scissions in DNA of Escherichia coli B/r cells induced by 20 krad gamma-radiation. The chemical altered the cell membrane structure as evidenced from the uptake of acriflavin, the efflux of potassium ions from the bacterial cells and the inhibition of alkaline phosphatase-a cell membrane associated enzyme. (author). 18 refs., 6 figures.

1982-09-01

385

Determination of the cell and mucous distribution in the airways of the lung

The analgesic, dipyrone (1,phenyl-2,3-dimethyl-5-pyrazolone-4-methylamino methane sulphonate sodium), at 20 mM concentration, inhibited the rejoining of single-strand scissions in DNA of Escherichia coli B/r cells induced by 20 krad gamma-radiation. The chemical altered the cell membrane structure as evidenced from the uptake of acriflavin, the efflux of potassium ions from the bacterial cells and the inhibition of alkaline phosphatase-a cell membrane associated enzyme. (author).

386

A robust cell voltage monitoring system for analysis and diagnosis of fuel cell or battery systems

Models of the human airways have played a major role in evaluating the health effects of inhaled radionuclides. While models such as those of Weibel (1963) provide data necessary for characterizing deposition of aerosol, they have not characterized the cells at risks in the airspaces. Given the advancements in techniques and study of cell cultures exposed to ionizing radiation there is a need to extrapolate between the simple structures of cell culture systems and the complex architecture of the human airways. The preliminary data in this paper provide a complete characterization of the size and number of cells in the airways and represents a significant advance in our study of the health consequences of exposure to inhaled radionuclides. 26 refs., 2 figs., 4 tabs.

1991-01-01

387

The radiosensitivity of spermatogonial stem cells in C3H/101 F[sub 1] hybrid mice

Cell voltage monitoring (CVM) systems are essential for the operation of fuel cell stacks and some battery systems, in the field as well as in the laboratory, because they allow the diagnosis and correction of problems that would otherwise go unnoticed and cause impaired performance or even permanent damage. A robust, safe, and low-cost design for a CVM unit is presented, using electromechanical relays as multiplexing switches. Some examples from the application of the unit on the University of Delaware's fuel cell battery hybrid buses are presented, including its use in automatically correcting anode flooding and diagnosing air channel blockage. (author)

2010-12-15

388

The effects of high-intensity pulsed electromagnetic field on proliferation and differentiation of neural stem cells of neonatal rats in vitro.

The radiosensitivity of spermatogonial stem cells of C3H/HeHx101/H F[sub 1] hybrid mice was determined by counting undifferentiated spermatogonia at 10 days after X-irradiation. During the spermatogenic cycle, differences in radiosensitivity were found, which were correlated with the proliferative activity of the spermatogonial stem cells. In stage VIII[sub irr], during quiescence, the spermatogonial stem cells were most radiosensitive with a D[sub 0] of 1.4 Gy. In stages XI[sub irr]-V[sub irr], when the cells were proliferatively active, the D[sub 0] was about 2.6 Gy. Based on the D[sub 0] values for sensitive and resistant spermatogonia and on the D[sub 0] for the total population, a ratio of 45:55% of sensitive to resistant spermatogonial stem cells was estimated for cell killing. When the present data were compared with data on translocation induction ...

1993-12-01

389

Solid oxide fuel cell simulation and design optimization with numerical adjoint techniques

The effects of high-intensity pulsed electromagnetic stimulation (HIPEMS) on proliferation and differentiation of neonatal rat neural stem cells in vitro were investigated. Neural stem cells derived from neonatal rats were exposed to 0.1 Hz, 0.5-10 Tesla (T) [8 groups of B-I, respectively], 5 stimuli of HIPEMF. The sham exposure controls were correspondingly established. Inverted phase contrast microscope was used to observe the cultured cells, MTT assay to detect the viability of the cells as expressed by absorbance (A) value, and flow cytometry to measure differentiation of neural stem cells. The results showed that A values of neural stem cells in both 3.0 T and 4.0 T groups were significantly higher than the other groups 24 to 168 h post HPEMS, indicating a strong promotion of the growth of neural stem cells (PHPEMS groups was the same ...

2009-12-29

390

Pre-oxidized and nitrided stainless steel alloy foil for proton exchange membrane fuel cell bipolar plates. Part 2: Single-cell fuel cell evaluation of stamped plates

This dissertation reports on the application of numerical optimization techniques as applied to fuel cell simulation and design. Due to the "multi-physics" inherent in a fuel cell, which results in a highly coupled and non-linear behavior, an experimental program to analyze and improve the performance of fuel cells is extremely difficult. This program applies new optimization techniques with computational methods from the field of aerospace engineering to the fuel cell design problem. After an overview of fuel cell history, importance, and classification, a mathematical model of solid oxide fuel cells (SOFC) is presented. The governing equations are discretized and solved with computational fluid dynamics (CFD) techniques including unstructured meshes, non-linear solution methods, numerical derivatives with complex variables, and sensitivity analysis with ...

2008-01-01

391

British Library Electronic Table of Contents (United Kingdom)

Thermal (gas) nitridation of stainless steel alloys can yield low interfacial contact resistance (ICR), electrically conductive and corrosion-resistant nitride containing surface layers (Cr2N, CrN, TiN, V2N, VN, etc.) of interest for fuel cells, batteries, and sensors. This paper presents results of proton exchange membrane (PEM) single-cell fuel cell studies of stamped and pre-oxidized/nitrided developmental Fe-20Cr-4V weight percent (wt.%) and commercial type 2205 stainless steel alloy foils. The single-cell fuel cell behavior of the stamped and pre-oxidized/nitrided material was compared to as-stamped (no surface treatment) 904L, 2205, and Fe-20Cr-4V stainless steel alloy foils and machined graphite of similar flow field design. The best fuel cell behavior among the alloys was exhibited...

2010-01-01

392

Natural killer cell activity influences outcome after T cell depleted stem cell transplantation from matched unrelated and haploidentical donors

British Library Electronic Table of Contents (United Kingdom)

Lytic activity and recovery of natural killer (NK) cells was monitored in pediatric patients with leukemias (ALL, AML, CML, JMML) and myelodysplastic syndromes after transplantation of T cell depleted stem cells from matched unrelated (n = 18) and mismatched related (haploidentical, n = 29) donors. CD34 + selection with magnetic microbeads resulted in 8 x 10^3/kg residual T cells. No post-transplant immune suppression was given. NK cells recovered rapidly after transplantation (300 CD56+/@mL at day 30, median), whereas T cell recovery was delayed (median: 12 CD3+/@mL at day 90). NK activity was measured as specific lysis of K 562 targets several times (mean: 3 assays per patient). Four temporal patterns of lytic activity could be differentiated: consistently low, consistently high, decreas...

2011-01-01

393

High-efficiency GaAs solar cells

Glycosaminoglycan synthesis by subpopulations of epithelial cells from a mammary adenocarcinoma

An updated review of the state of the art in the development of GaAs solar cells is provided, with emphasis on AlGaAs-GaAs cells suitable for space applications. A set of theoretically derived characteristics is given for this type of solar cell. Comparison of measured performance with theory shows excellent agreement. Data on the effects of radiation damage (high-energy electrons, protons, and neutrons) is also integrated into a form useful for evaluation purposes. Techniques for fabricating (AlGa)As-GaAs solar cells in quantities large enough for practical applications are discussed and are shown to have been demonstrated. The possibility of extending these techniques to the fabrication of very thin low-weight cells for space applications is also considered. Finally, the results obtained to date in the development of GaAs solar cells for applications requiring ...

1984-05-01

394

Effect of chronic fractionated low-dose gamma irradiation on division potential of human embryonic cells in vitro

Glycosaminoglycan synthesis by two subpopulations of a mouse mammary tumor cell line was compared. The two sublines express distinctly different growth characteristics in vitro and in vivo which indicate differences in growth regulation. Newly made glycosaminoglycans were recovered from the culture media, the cell surfaces, and residual cellular material. The cell population which grows more aggressively in vivo (+SA subline, a subline that grows in soft agarose) incorporated about 8 times more (/sup 14/C)glucosamine per cell into total glycosaminoglycans than did the slower-growing population (-SA subline, which does not grow in soft agarose). Appropriate control experiments indicated that the apparent difference in rates of synthesis was not due to discrepancies in glucosamine uptake. The main residual cellular molecule labeled was heparan sulfate, but the predominant molecule at the ...

1982-06-01

395

Effect of Nucleus Pulposus Cells Having Different Phenotypes on Chondrogenic Differentiation of Adipose-Derived Stromal Cells in a Coculture System Using Porous Membranes

We investigated the in vitro phenotypic transformation of human embryo (HE) cells that were repeatedly irradiated (7.5 cGy once a week) throughout their life-span. Irradiation was repeated until the cells had accumulated 195 cGy (equivalent to the 26th passage). Samples of cells were assayed for survival by colony formation, as well as for mutation at the hypoxanthine guanine phosphoribosyl transferase (HGPRT) locus and for transformation by focus formation. The life-span (mean number of population doublings) of multiply irradiated cells with a total dose of 97.5 cGy was slightly but significantly prolonged over that of controls. After HE cells had accumulated 195 cGy, the maximum number of divisions increased to 130-160% of the number in non-irradiated control cells. Transformed foci were not observed until cells had accumulated 97.5 cGy, ...

1991-12-01

396

British Library Electronic Table of Contents (United Kingdom)

In this study, adipose-derived stem cells (ASCs) were cocultured with nucleus pulposus (NP) cells using a porous membrane to investigate the effect of NP cell phenotype on ASC chondrogenic differentiation. Human NP cells were collected from 14 patients and classified into two groups (normal vs. degenerative) depending on the level of type II collagen, aggrecan (AGG), type I collagen, and bax gene expression. Human ASCs were then cocultured with each group of NP cells on porous membranes in the absence of chondrogenic supplements. After 2 weeks, real-time-polymerase chain reaction results showed that ASCs cocultured with normal NP cells had much higher type II collagen and AGG gene expression than ASCs cocultured with degenerative NP cells. The production of AGG was also observed only in th...

2011-01-01

397

Cell behaviour on chemically microstructured surfaces

Assessing the risk of ovarian hyperstimulation syndrome in egg donation: implications for human embryonic stem cell research.

Micropatterned surfaces with different chemical topographies were synthesised in order to investigate the influence of surface chemistry and topography on cell behaviour. The microstructured materials were synthesised by photoimmobilising natural Hyaluronan (Hyal) and its sulphated derivative (HyalS), both adequately functionalised with a photorective moiety, on glass substrates. Four different grating patterns (10, 25, 50 and 100 {mu}m) were used to pattern the hyaluronan. The micropatterned samples were analysed by Secondary Ions Mass Spectrometry, Scanning Electron Microscopy (SEM) and Atomic Force Microscopy to investigate the chemistry and the topography of the surfaces. The spectroscopic and microscopic analysis of the microstructured surfaces revealed that the photoimmobilisation process was successful, demonstrating that the photomask patterns were well reproduced on the sample surface. The influence of chemical topographies on the cell ...

2003-03-03

398

15th Workshop on Crystalline Silicon Solar Cells and Modules: Materials and Processes; Extended Abstracts and Papers

Stem cell research has important implications for medicine. The source of stem cells influences their therapeutic potential, with stem cells derived from early-stage embryos remaining the most versatile. Somatic cell nuclear transfer (SCNT), a source of embryonic stem cells, allows for understandings about disease development and, more importantly, the ability to yield embryonic stem cell lines that are genetically matched to the somatic cell donor. However, SCNT requires women to donate eggs, which involves injection of ovulation-inducing hormones and egg retrieval through laparoscopy or transvaginal needle aspiration. Risks from this procedure are fiercely debated, most notably risk of ovarian hyperstimulation syndrome (OHSS). This review examines risk of OHSS resulting from oocyte donation. We conclude that risk posed by OHSS in egg ...

2011-09-01

399

Variation in the sensitivity of the mouse spermatogonial stem cell population to fission neutron irradiation during the cycle of the seminiferous epithelium

The National Center for Photovoltaics sponsored the 15th Workshop on Crystalline Silicon Solar Cells & Modules: Materials and Processes, held in Vail, CO, August 7-10, 2005. This meeting provided a forum for an informal exchange of technical and scientific information between international researchers in the photovoltaic and relevant non-photovoltaic fields. The workshop addressed the fundamental properties of PV silicon, new solar cell designs, and advanced solar cell processing techniques. A combination of oral presentations by invited speakers, poster sessions, and discussion sessions reviewed recent advances in crystal growth, new cell designs, new processes and process characterization techniques, and cell fabrication approaches suitable for future manufacturing demands. The theme of this year's meeting was 'Providing the Scientific Basis for Industrial ...

2005-11-01

400

Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation.

Dose-response studies of the radiosensitivity of spermatogonial stem cells in various epithelial stages after irradiation with graded doses of fission neutrons of 1 MeV mean energy were carried out in the Cpb-N mouse. These studies on the stem cell population in stages IX-XI yielded simple exponential lines characterized by an average D0 value of 0.76 +/- 0.02 Gy. In the subsequent epithelial stages XII-III, a significantly lower D0 value of 0.55 +/- 0.02 Gy was found. In contrast to the curves obtained for stem cells in stages IX-III, the curves obtained in stages IV-VIII indicated the presence of a mixture of radioresistant and radiosensitive stem cells. In stage VII, almost no radioresistant stem cells appeared to be present and a D0 value for the radiosensitive stem cells of 0.22 +/- 0.01 Gy was derived. Previously, data were obtained on the size of colonies ...

1986-12-01

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401

To Get Overall Shapes and New Data of the 120-Cell and the 600-Cell

Multicellular tumor spheroids (MCTS) are three dimensional cell culture systems induced by suspension culture. MCTS are widely used in cancer research because of their similarity to solid tumors. CaSki cells are derived from a metastatic cervical cancer containing human papillomavirus 16 (HPV16). Cell death of CaSki cells in MCTS has been previously reported, and our model is used to better characterize the mechanisms of cell death of HPV16-positive keratinocytes. In this study, we found that apoptosis of CaSki cells was induced by suspension culture along with the formation of MCTS after 24 h of incubation. In suspended CaSki cells, monoclonal antibodies blocking E-cadherin function inhibited MCTS formation and suppressed suspension-induced apoptosis in a dose-dependent manner. Western blot for E-cadherin detected upregulation of the ...

2008-01-01

402

Thyroid cancer stem cells

This research will be helpful for people to display the 2-dimensiona projective models of 4-variable actual problems in many fields, in order to investigate deeply those actual problems. By using the theory of N-dimensional finite rotation group of the regular polytopes, the author established the 2-dimensional projective model of 4-dimensional rectangular coordinate system, and deduced a transformation matrix, and adopt it to display successfully the 2-dimensional overall shapes of two most complicated regular polytopes 120-Cell and 600-Cell. In the meantime, the author calculated all the vertex coordinates and determine the joint relationships between adjacent vertices of the regular polytopes 120-Cell and 600-Cell. Also, this provided a pattern for displaying the 2-dimensional projective model of 4-variable actual problem.

2009-01-01

403

British Library Electronic Table of Contents (United Kingdom)

Thyroid cancer is the most frequently diagnosed endocrine cancer and causes more deaths than all other endocrine cancers combined. Research findings support the concept that a subpopulation of thyroid cancer cells displays properties characteristic of stem cells. These putative cancer-forming entities drive tumorigenesis as a result of their dual ability to undergo self-renewal and to differentiate into various types of cancer cells; they also mediate metastasis and are resistant to the effects of chemotherapy and radiation therapy. This Review discusses the cellular origin of thyroid cancer and the properties of the thyroid cancer stem cell niche. The article critically evaluates the methods used to identify molecular markers expressed by thyroid-cancer-initiating cells and outlines prosp...

2011-01-01

404

Stem Cells, Nitrogen-Rich Plasma-Polymerized Culture Surfaces, and Type X Collagen Suppression

British Library Electronic Table of Contents (United Kingdom)

Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into chondrocytes, osteoblasts, myocytes, adipocytes, and a variety of other cell types. Several studies have been directed toward using MSCs from patients with osteoarthritis (OA) for cartilage repair, not only because these are the ones that will require a source of autologous stem cells if biological repair of cartilage lesions is to be a therapeutic option, but also to further an understanding of stem cell differentiation. Previous studies have shown that a major drawback of current cartilage and intervertebral disc tissue repair is that human MSCs from OA patients express type X collagen (COL X). COL X, a marker of late-stage chondrocyte hypertrophy, is implicated in endochondral ossification. However, those st...

2011-01-01

405

Role of regulatory T cell populations in controlling graft vs host disease

British Library Electronic Table of Contents (United Kingdom)

Immune function is critical in health and disease. The control and regulation of immune reactions is an area of intense investigation that has important implications for allogeneic hematopoietic cell transplantation. Immune reactions are regulated in a number of important ways. Compartmentalization of immune responses and the production of both pro-inflammatory and anti-inflammatory cytokines play a major role. More recently several populations of T cells that regulate immune responses termed regulatory T cells have been identified. This manuscript will focus on CD4^+CD25^+FoxP3^+ natural regulatory T cells (T"r"e"g) and @a@bTCR^+CD4^+NK1.1^+ natural killer T (NK-T) cells which both suppress graft vs host disease but appear to function by distinct mechanisms.

2011-01-01

406

Results of cycling with battery charging management; Resultats de cyclage avec gestion de charge au niveau batterie

Nickel induced structural and functional alterations in mouse Leydig cells in vitro

In order to investigate the charging mode of an in-series assembly of lithium-carbon battery cells, a test has been performed on 5 commercial cells (18650) of 0.95 Ah nominal capacity. Results show that it is possible to cycle the cells at 80% of their output capacities during more than 2000 cycles. The management of the battery consists in maintaining a constant battery voltage as soon as a cell reaches its limit voltage during constant current charging. The initial dispersion of cells has been maintained practically constant during the cycling and the charge state of all cells has decreased progressively. (J.S.)

1996-12-31

407

British Library Electronic Table of Contents (United Kingdom)

The present study was aimed at investigating effects of nickel (NiCl2) on secretion of testosterone (T), cell viability, ultrastructure and apoptosis in mouse Leydig cells. Testosterone release was measured after 48h of culture with 15.67, 31.25, 62.5, 125, 250, 500 and 1000mmol/L NiCl2 or without NiCl2 using radioimmunoassay. Cell viability was assessed by a MTT (metabolic activity assay). Quantification of apoptotic cells was performed using TUNEL assay and the ultrastructural changes were analyzed using transmission electron microscopy. The viability was decreased after addition of >=250mmol/L NiCl2. A concentration-dependent depression of T production was observed. The percentage of apoptotic cells was significantly increased only after addition of 125, 250 and 1000mmol/L NiCl2. After ...

2011-01-01

408

MOCVD growth of GaAs solar cells on silicon substrates

Life span of multipotential hematopoietic stem cells in vivo

This paper reports advances in the development of solar cells made from GaAs-on-Si structures prepared by metalorganic chemical vapor deposition (MOCVD). The use of concentrator cells, operating at [similar to]200 suns, has led to the efficiency achievements of 21.3% (AM1.5D) for a GaAs-on-Si solar cell, and 27.6 (AM1.5D) for a homoepitaxial GaAs cell. The development of epitaxial multilayer dielectric mirrors (Bragg reflectors), as back-surface reflectors in thin-film GaAs cells, on both Si and GaAs substrates, is shown to lead to modest efficiency increases, over that of conventional designs.

1992-12-01

409

Layer- and cell-type-specific suprathreshold stimulus representation in rat primary somatosensory cortex

The findings reported in this study highlight several important features of the development of hematopoietic stem cells after transplantation into irradiated recipients. First, they demonstrate the existence of a class of primitive multipotential stem cells that can function for a significant portion of the lifetime of a mouse (15 mo). In addition, they clearly show that these primitive stem cells can be infected with recombinant retroviruses and thus would be appropriate targets for gene therapy in somatic tissues. Second, our data indicate that the progeny of some, but not all, of the primitive stem cells have fully expanded into the various hematopoietic lineages by 2 mo after reconstitution. Finally, our analysis of the secondary recipients provides strong evidence suggesting that the primitive stem cell population can actually clonally expand. Our current experiments are aimed ...

1990-05-01

410

British Library Electronic Table of Contents (United Kingdom)

Sensory stimuli are encoded differently across cortical layers and it is unknown how response characteristics relate to the morphological identity of responding cells. We therefore juxtasomally recorded action potential (AP) patterns from excitatory cells in layer (L) 2/3, L4, L5 and L6 of rat barrel cortex in response to a standard stimulus (e.g. repeated deflection of single whiskers in the caudal direction). Subsequent single-cell filling with biocytin allowed for post hoc identification of recorded cells. We report three major conclusions. First, sensory-evoked responses were layer- and cell-type-specific but always

2007-01-01

411

Laser-assisted solar cell metallization processing. Annual report, December 13, 1984-September 30, 1985

Ionizing radiation is a potent inducer of mitotic recombination in mouse embryonic stem cells

In this contract, Laser-assisted processing techniques for producing high-quality solar cell metallization patterns are being investigated, developed, and characterized. The tasks comprising these investigations are outlined. Four new batches of solar cells were processed, in addition to several test runs on wafers, using the laser decomposition of spun-on silver neodecanoate to metallize cells. Decomposition of silver neodecanoate was carried out at different laser powers on different cells on a given wafer to determine whether this would have any effect on cell performance. A one watt laser power gave an electroplated linewidth of 50 ..mu..m, while at 8 watts the line width was 90 ..mu..m.

1986-01-06

412

British Library Electronic Table of Contents (United Kingdom)

Maintenance of genomic integrity in embryonic cells is pivotal to proper embryogenesis, organogenesis and to the continuity of species. Cultured mouse embryonic stem cells (mESCs), a model for early embryonic cells, differ from cultured somatic cells in their capacity to remodel chromatin, in their repertoire of DNA repair enzymes, and in the regulation of cell cycle checkpoints. Using 129XC3HF1 mESCs heterozygous for Aprt, we characterized loss of Aprt heterozygosity after exposure to ionizing radiation. We report here that the frequency of loss of heterozygosity mutants in mESCs can be induced several hundred-fold by exposure to 5-10Gy of X-rays. This induction is 50-100-fold higher than the induction reported for mouse adult or embryonic fibroblasts. The primary mechanism underlying the...

2011-01-01

413

Green Tea Extract (Epigallocatechin-3-Gallate) Reduces Efficacy of Radiotherapy on Prostate Cancer Cells

British Library Electronic Table of Contents (United Kingdom)

ObjectivesTo assess the influence of epigallocatechin-3-gallate (EGCG) on the efficacy of ionizing radiation on prostate cancer cells because of the increased use of dietary interventions, especially by patients with prostate cancer. Radiotherapy is used to treat localized prostate cancer. Some people consume green tea (EGCG) as a chemopreventive agent against prostate cancer. Green tea can act as an antioxidant and induce superoxide dismutase enzymes, which could scavenge the free oxygen radicals generated by radiotherapy. MethodsProstate cancer cell line DU145 cells were treated with EGCG or radiotherapy, or both. Cell death was assessed using trypan blue cell counting, and apoptosis was confirmed by assessing poly (adenosine phosphate ribose) polymerase cleavage. The antioxidant potenti...

2011-01-01

414

Gonadal hormone modulation of neurogenesis in the dentate gyrus of adult male and female rodents

British Library Electronic Table of Contents (United Kingdom)

Gonadal hormones modulate neurogenesis in the dentate gyrus differentially in male and female adult rodents. Neurogenesis is comprised of at least two components: cell proliferation (the production of new cells) and cell survival (the number of new neurons that survive to maturity). Previous studies have found sex differences in the level of cell proliferation in the dentate gyrus only when comparing females in a high estrogen state to males. This review focuses on the effects of acute and chronic levels of estrogens or androgens on hippocampal neurogenesis in the adult male and female rodent. Evidence is also reviewed for the co-localization of androgen receptors and estrogen receptors (ER) with markers for cell proliferation or immature new cell survival. Briefly, evidence suggests that ...

2008-01-01

415

Functionally defined substates within the human embryonic stem cell compartment.

Flow cytometric analysis of respiratory tract cells exposed to oil shale and silica particulates. [Hamsters

Human embryonic stem (ES) cells can undergo spontaneously differentiation in standard culture conditions, demonstrating that the undifferentiated state is relatively unstable. The heterogeneous expression of SSEA3 observed within human ES colonies, provides a means to examine undifferentiated stem cell substates. Through functional testing of single cells we have shown that undifferentiated ES cells can be segregated into functionally discrete subpopulations on the basis of SSEA3 expression: SSEA3(High), SSEA(Low) and SSEA3(Negative). Human ES subpopulations were found to be interconvertible, but they possess distinct properties when challenged to differentiate along the neural lineage. These data suggest that ES cells with pluripotent/self-renewal capacities can exhibit different responses to induction of differentiation. PMID:21763622

2011-05-11

416

Electrochemical and gas phase parameters of cathodes for intermediate temperature solid oxide fuel cells

Flow cytometric techniques were used to measure the cytological and biochemical damage to respiratory tract cells in animals exposed to particulates. Hamsters were exposed to raw and spent oil shale particulates and silica by intratracheal instillation. Exfoliated lung cells were obtained by sacrificing the animals and lavaging the respiratory tract posterior to the trachea with saline. Cell samples were fixed in ethanol and stained with mithramycin for fluorescence analysis of DNA content. DNA content distributions from hamsters exposed to spent oil shale and silica particulates showed atypical changes 28 to 35 days later. Cell counts and total numbers of macrophages, leukocytes, and epithelial cells in the lavage fluid also showed marked changes related to time after exposure.

1979-01-01

417

Effects of cell culture conditions on antibody N-linked glycosylation-what affects high mannose 5 glycoform

A series of cyclic voltammetry, chronoamperometry and electrochemical impedance experiments have been carried out in order to investigate the effect of cathode composition and porosity on the electrochemical characteristics of strontium-doped lanthanum, praseodymium and gadolinium cobaltite cathodes. The impedance responses at different electrode potentials of the half cell and symmetric single cell setups are compared and analyzed by the equivalent circuit modeling method. The deconvolution of impedance spectra for single cell cathode and anode reactions contributions based on the results of simultaneous analysis of half cells and symmetric single cells has been made by differential impedance real part vs. ac frequency plot analysis method. Noticeable influence of cathode chemical composition, meso-porosity and macro-porosity on the electrochemical activity of the oxygen ...

2010-11-01

418

British Library Electronic Table of Contents (United Kingdom)

Abstract The glycosylation profile of therapeutic antibodies is routinely analyzed throughout development to monitor the impact of process parameters and to ensure consistency, efficacy, and safety for clinical and commercial batches of therapeutic products. In this study, unusually high levels of the mannose-5 (Man5) glycoform were observed during the early development of a therapeutic antibody produced from a Chinese hamster ovary (CHO) cell line, model cell line A. Follow up studies indicated that the antibody Man5 level was increased throughout the course of cell culture production as a result of increasing cell culture medium osmolality levels and extending culture duration. With model cell line A, Man5 glycosylation increased more than twofold from 12% to 28% in the fed-batch process...

2011-01-01

419

Effect of various coal gas contaminants on the performance of solid oxide fuel cells: Part III. Synergistic effects

British Library Electronic Table of Contents (United Kingdom)

The coal-derived gas from a coal gasifier contains multiple contaminants, and their synergistic effects may not be simply the additive influences of individual contaminants. The present work presents the results of a study of the synergistic effects of four contaminants of major concern-S, As, P, and Cl, at the ppm level and in combinations of two, three, or four kinds-on the performance of Ni-YSZ/YSZ/LSM solid oxide fuel cells. The results indicate that both cell performance and morphology differ significantly in cells exposed to a single contaminant, and that cell performance is not simply the additive influence of each contaminant. Synergistic effects can be very destructive (accelerated degradation or even cell failure) when S is in the presence with As/P, but can also be beneficial (s...

2010-01-01

420

Effect of Relative Humidity on Deinococcus radiodurans? Resistance to Prolonged Desiccation, Heat, Ionizing, Germicidal, and Environmentally Relevant UV Radiation

British Library Electronic Table of Contents (United Kingdom)

To test the effect of humidity on the radiation resistance of Deinococcus radiodurans, air-dried cells were irradiated with germicidal 254?nm UV, and simulated environmental UV or ?-radiation and survival was compared to cells in suspension. It was observed that desiccated cells exhibited higher levels of resistance than cells in suspension toward UV or ?-radiation as well as after 85?C heat shock. It was also shown that low relative humidity improves survival during long-term storage of desiccated D. radiodurans cells. It can be concluded that periods or environments in which cells exist in a dehydrated state are beneficial for D. radiodurans? survival exposed to various other stresses.

2011-01-01

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421

Deep-level defects and numerical simulation of radiation damage in GaAs solar cells

Cell fate regulation by coupling mechanical cycles to biochemical signaling pathways

A review of the deep-level defects observed in both electron- and proton-irradiated GaAs solar cells is presented. Studies of the effects of periodic and continuous thermal annealing on the radiation-induced electron and hole traps and the recombination parameters in GaAs solar cells were made for a wide range of electron and proton energies, fluence, annealing temperature and annealing time. A refined model for numerical simulations of the displacement damage was developed for computing the defect density and the cell parameters in the electron- and proton-irradiated GaAs solar cells. Excellent agreement was obtained between the calculated values and the experimental data for the proton-irradiated GaAs solar cells. (orig.).

1991-09-01

422

British Library Electronic Table of Contents (United Kingdom)

Many aspects of cellular motility and mechanics are cyclic in nature such as the extension and retraction of lamellipodia or filopodia. Inherent to the cycles of extension and retraction that test the environment is the production of mechano-chemical signals that can alter long-term cell behavior, transcription patterns, and cell fate. We are just starting to define such cycles in several aspects of cell motility, including periodic contractions, integrin cycles of binding and release as well as the normal oscillations in motile activity. Cycles of local cell contraction and release are directly coupled to cycles of stressing and releasing extracellular contacts (matrix or cells) as well as cytoplasmic mechanotransducers. Stretching can alter external physical properties or sites exposed b...

2009-01-01

423

Atrazine exposure leads to altered growth of HepG2 cells

British Library Electronic Table of Contents (United Kingdom)

Atrazine is one of the most commonly used herbicides in the United States. While effective on target plants, it has been associated with harmful health effects in non-target organisms such as fish, amphibians and mammals. In this study, growth effects on human liver cells were determined after exposure to increasing concentrations of this herbicide. Growth of immortalized human hepatoma HepG2 cells was inhibited by atrazine concentrations of 625 ppb after 72 h exposure and flow cytometry analysis demonstrated HepG2 cells exposed to 100 ppb atrazine accumulated in S phase after 48 h compared to untreated cells. Expression of cell cycle specific cyclin proteins was altered after atrazine exposure with cyclin E levels significantly decreased after a 24 h exposure and cyclin B levels decreased...

2011-01-01

424

A mechanistic understanding of production instability in CHO cell lines expressing recombinant monoclonal antibodies

British Library Electronic Table of Contents (United Kingdom)

Abstract One of the most significant problems in industrial bioprocessing of recombinant proteins using engineered mammalian cells is the phenomenon of cell line instability, where a production cell line suffers a loss of specific productivity (qP). This phenomenon occurs with unpredictable kinetics and has been widely observed in Chinese hamster ovary (CHO) cell lines and with all commonly used gene expression systems. The underlying causes (both genetic and physiological) and the precise molecular mechanisms underpinning cell line instability have yet to be fully elucidated, although recombinant gene silencing and loss of recombinant gene copies have been shown to cause qP loss. In this work we have investigated the molecular mechanisms underpinning qP instability over long-term sub-cult...

2011-01-01

425

Nonphotochemical Hole-Burning Imaging Studies of in vitro Carcinoma and Normal Cells Utilizing a Mitochondrial Specific Dye

Nonphotochemical Hole-Burning Imaging Studies of In Vitro Carcinoma and Normal Cells Utilizing a Mitochondrial Specific Dye

Low temperature Nonphotochemical Hole Burning (NPHB) Spectroscopy of the dye rhodamine 800 (MF680) was applied for the purpose of discerning differences between cultured normal and carcinoma ovarian surface epithelial (OSE) cells. Both the cell lines were developed and characterized at the Mayo Clinic (Rochester, MN), with the normal cell line having been transfected with a strain of temperature sensitive Simian Virus 40 Large T Antigen (SV40) for the purpose of extending the life of the cell culture without inducing permanent changes in the characteristics of the cell line. The cationic lipophilic fluorophore rhodamine 800 preferentially locates in in situ mitochondria due to the high lipid composition of mitochondria and the generation of a large negative membrane potential (relative to the cellular cytoplasm) for oxidative phosphorylation. Results presented for NPHB of MF680 ...

2002-06-27

426

The p75"N"T"R tumor suppressor induces cell cycle arrest facilitating caspase mediated apoptosis in prostate tumor cells

Low temperature Nonphotochemical Hole Burning (NPHB) Spectroscopy of the dye rhodamine 800 (MF680) was applied for the purpose of discerning differences between cultured normal and carcinoma ovarian surface epithelial (OSE) cells. Both the cell lines were developed and characterized at the Mayo Clinic (Rochester, MN), with the normal cell line having been transfected with a strain of temperature sensitive Simian Virus 40 Large T Antigen (SV40) for the purpose of extending the life of the cell culture without inducing permanent changes in the characteristics of the cell line. The cationic lipophilic fluorophore rhodamine 800 preferentially locates in in situ mitochondria due to the high lipid composition of mitochondria and the generation of a large negative membrane potential (relative to the cellular cytoplasm) for oxidative phosphorylation. Results presented for NPHB of MF680 ...

2002-08-01

427

Processing of asparagine-linked oligosaccharides in HT-29 cells is a function of their state of enterocytic differentiation: an accumulation of Man/sub 9-8/-GlcNAc_2-Asn species is indicative of an impaired N-glycan trimming in undifferentiated cells

The p75 neurotrophin receptor (p75"N"T"R) is a death receptor which belongs to the tumor necrosis factor receptor super-family of membrane proteins. This study shows that p75"N"T"R retarded cell cycle progression by induced accumulation of cells in G0/G1 and a reduction in the S phase of the cell cycle. The rescue of tumor cells from cell cycle progression by a death domain deleted (#DELTA#DD) dominant-negative antagonist of p75"N"T"R showed that the death domain transduced anti-proliferative activity in a ligand-independent manner. Conversely, addition of NGF ligand rescued retardation of cell cycle progression with commensurate changes in components of the cyclin/cdk holoenzyme complex. In the absence of ligand, p75"N"T"R-dependent cell cycle arrest facilitated an increase in apoptotic nuclear fragmentation of the prostate cancer ...

2006-03-24

428

On the cells of origin of radiogenic thyroid cancer: New studies based on an old idea

Studies on the regulation of the enterocytic differentiation of the human colon cancer cell line HT-29, which is differentiated in the absence (Glc"-) but not in the presence of glucose (Glc"+), have recently shown that the post-translational processing of sucrase-isomaltase and particularly its glycosylation vary as a function of cell differentiation. Other studies indicate that in undifferentiated HT-29 Glc"+ cells there is an accumulation of UDP-N-acetylhexosamine, which is involved in the glycosylation process. The purpose of the present work is to investigate whether an overall alteration of protein glycosylation is associated with the inability of HT-29 cells to differentiate. At least three alterations are detected: (i) after a 10-min pulse, the incorporation of D-[2-"3H]mannose in undifferentiated cells is severely reduced, compared to differentiated ...

429

Olfactomedin 4 suppresses prostate cancer cell growth and metastasis via negative interaction with cathepsin D and SDF-1.

We have presented evidence that the functional thyroid follicles (follicular units, FU) which are formed in grafts of monodispersed rat thyroid cells, and hence the thyroid tumors which later develop in such grafts, are clonal in origin. Recent studies have been designed to investigate: whether cell number-dependent inhibition of promotion-progression is mediated by remote hormonal feed-back, local cell-cell interactions, or both; the cell population kinetics of the clonogen subpopulation during goitrogenesis and goiter involution; and the effect of prolonged exposure to high levels of TSH (thyrotropin) on the capacity of the clonogens to give rise to functional FU. The results indicate that local cell-cell interactions play an important role in the cell number-dependent suppression of neoplastic promotion-progression. They also show that if sufficient thyroid ...

1990-01-01

430

Nuclear heparan sulfate and doubling time in hepatocytes

The human olfactomedin 4 gene (OLFM4) encodes an olfactomedin-related glycoprotein. OLFM4 is normally expressed in a limited number of tissues, including the prostate, but its biological functions in prostate are largely unknown. In this study, we found that OLFM4 messenger RNA was reduced or undetectable in prostate cancer tissues and prostate cancer cell lines. To study the effects of OLFM4 on prostate cancer progression, we transfected PC-3 prostate cancer cells with OLFM4 to establish OLFM4-expressing PC-3 cell clones. The OLFM4-expressing PC-3 cell clones were found to have decreased proliferation and invasiveness compared with vector-transfected control PC-3 cells in vitro. In addition, nude mice injected with OLFM4-expressing PC-3 cells demonstrated reduced tumor growth and bone invasion and metastasis compared with mice injected with vector-transfected ...

2011-04-05

431

Honokiol-mediated inhibition of PI3K/mTOR pathway: a potential strategy to overcome immunoresistance in glioma, breast, and prostate carcinoma without impacting T cell function.

A rat hepatocyte cell line was shown previously to contain a nuclear pool of free heparan SO/sub 4/ (HS) chains which turns over rapidly. These cells were labeled with /sup 35/SO/sub 4//sup 2 -/ and the steady state levels of (/sup 35/SO/sub 4/)HS in the nucleus were measured under culture conditions chosen to give different cell doubling times (DT's). In each log phase culture growing at a constant rate, the level of nuclear HS did not change as the cell density increased. Rapidly dividing cells had levels of nuclear HS within the range of 30-42 pmol /sup 35/SO/sub 4/ in the nuclear HS/10/sup 6/ cells. However, in cultures with decreased DT's, there was a corresponding decrease in the level of nuclear HS below 30 pmol/10/sup 6/ cells that was proportional to the decrease in the DT. In contrast, when the ...

1987-05-01

432

Inhibition of the phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway is an appealing method for decreasing the immunoresistance and augmenting T cell-mediated immunotherapy. A major impediment to this strategy is the impact of conventional PI3K/mTOR pathway inhibitors on T cell function. In particular, rapamycin, is a well-known immunosuppressant that can decrease the activity of the PI3K/mTOR pathway in tumor cells, but also has a profound inhibitory effect on T cells. Here we show that Honokiol, a natural dietary product isolated from an extract of seed cones from Magnolia grandiflora, can decrease PI3K/mTOR pathway-mediated immunoresistance of glioma, breast and prostate cancer cell lines, without affecting critical proinflammatory T cell functions. Specifically, we show that at doses sufficient to down-regulate levels of phospho-S6 ...

433

A model of episodic memory: mental time travel along encoded trajectories using grid cells.

Glycosaminoglycan synthesis was studied in cell populations of ultraviolet light-induced murine cutaneous fibrosarcoma cells under conditions of varying growth rates in vitro. After labeling with the precursors, /sup 3/H-glucosamine and /sup 35/SO/sub 4/, sulfated glycosaminoglycans recoverable by direct proteolysis of the culture monolayers increased approximately 5-fold on a per cell basis from sparsely populated, exponential cell cultures (greater than 85% of cells in S, G2, or M phases) to stationary cultures inhibited by high cell density (greater than 50% of cells in G1). Within this cell surface-associated material, the relative ratio of heparan sulfate to the chondroitin sulfates was approximately 60/40% under conditions of exponential growth; in the growth-arrested cultures, the reverse ratio was found. The ...

1985-08-01

434

On the impact of low power density microwaves in some living tissues

The definition of episodic memory includes the concept of mental time travel: the ability to re-experience a previously experienced trajectory through continuous dimensions of space and time, and to recall specific events or stimuli along this trajectory. Lesions of the hippocampus and entorhinal cortex impair human episodic memory function and impair rat performance in tasks that could be solved by retrieval of trajectories. Recent physiological data suggests a novel model for encoding and retrieval of trajectories, and for associating specific stimuli with specific positions along the trajectory. During encoding in the model, external input drives the activity of head direction cells. Entorhinal grid cells integrate the head direction input to update an internal representation of location, and drive hippocampal place cells. Trajectories are encoded by Hebbian modification of excitatory synaptic connections between ...

2009-07-15

435

var gene transcription and PfEMP1 expression in the rosetting and cytoadhesive Plasmodium falciparum clone FCR3S1.2

The biomedical and ecological interest for the microwave impact on the Earth biosphere is continuously increased since the industrial, military and communication activities strongly contribute to the electromagnetic stress of living bodies. In the next the authors present some of the main results obtained regarding the microwave exposure of various types of biological material: bacteria, fungi, young plant seedlings, dry seeds, animal tissues. The electromagnetic exposure was carried out in open space in well controlled environmental conditions by using 10.75 GHz/1 m W cm{sup -2} microwaves. Biochemical assays and cytogenetic tests have been carried out to reveal the changes induced post irradiation. The response of some pathogen bacteria, have been emphasized by means of turbidimetric measurements - the stimulatory effect being noticed at the level of the microbial population density (the stimulation of the human body microbial flora seems to be one of the side effects of microwave ...

2006-07-01

436

Wiskott-Aldrich syndrome protein is required for regulatory T cell homeostasis

BackgroundThe pathogenicity of Plasmodium falciparum is in part due to the ability of the parasitized red blood cell (pRBC) to adhere to intra-vascular host cell...Full Text Available

437

Variable domain I of nematode CLEs directs post-translational targeting of CLE peptides to the extracellular space

Wiskott-Aldrich syndrome protein (WASp) is essential for optimal T cell activation. Patients with WAS exhibit both immunodeficiency and a marked susceptibility to systemic autoimmunity. We investigated...Full Text Available

2007-02-01

438

Unusual Topological Arrangement of Structural Motifs in the Baboon Reovirus Fusion-Associated Small Transmembrane Protein

Effector proteins expressed in the esophageal gland cells of cyst nematodes are delivered into plant cells through a hollow, protrusible stylet. Although evidence indicates that effector proteins function...Full Text Available

2010-12-01

439

Tumor vascular permeability factor stimulates endothelial cell growth and angiogenesis.

Select members of the Reoviridae are the only nonenveloped viruses known to induce syncytium formation. The fusogenic orthoreoviruses accomplish cell-cell fusion through a distinct...Full Text Available

2005-05-01

440

Transplantation of allogeneic T cells alters iron homeostasis in NOD/SCID mice

Vascular permeability factor (VPF) is an Mr 40-kD protein that has been purified from the conditioned medium of guinea pig line 10 tumor cells grown in vitro, and increases fluid permeability from blood...Full Text Available

1989-11-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

441

Three-Dimensional Traction Force Microscopy: A New Tool for Quantifying Cell-Matrix Interactions

Iron overload is common in patients undergoing allogeneic hematopoietic cell transplantation (HCT), but the mechanisms leading to overload are unknown. Here, we determined iron levels and the expression...Full Text Available

2009-02-19

442

The p14 FAST Protein of Reptilian Reovirus Increases Vesicular Stomatitis Virus Neuropathogenesis?

The interactions between biochemical processes and mechanical signaling play important roles during various cellular processes such as wound healing, embryogenesis, metastasis, and cell migration. While...Full Text Available

443

The frequency of CD25+CD4+ and FOXP3+ regulatory T cells in ectopic endometrium and ectopic decidua

The fusogenic orthoreoviruses express nonstructural fusion-associated small transmembrane (FAST) proteins that induce cell-cell fusion and syncytium formation. It has been speculated that the FAST proteins...Full Text Available

2009-01-01

444

The biological and clinical significance of HCG-containing cells in seminoma.

BackgroundThe presence of regulatory T (Treg) cells in human endometrium is crucial for maintaining immunological homeostasis within the uterus. For this study we decided to evaluate...Full Text Available

445

Slug down-regulation by RNA interference inhibits invasion growth in human esophageal squamous cell carcinoma

The morphological appearance, incidence and prognostic significance of human chorionic gonadotrophin (HCG)-containing cells in seminomas were examined in a retrospective series of 228 orchidectomy specimens,...Full Text Available

1985-04-01

446

Role of bile salt in regulating Mcl-1 phosphorylation and chemoresistance in hepatocellular carcinoma cells

BackgroundEsophageal squamous cell carcinoma (ESCC) is one of the most aggressive carcinomas of the gastrointestinal tract. We assessed the relevance of Slug in measuring the invasive...Full Text Available

447

Regulation of Calcium Influx in Chara¹

BackgroundGlycochenodeoxycholate (GCDA) is one of the major human bile salts. Bile salts stimulate cell survival and proliferation through the mitogen-activated protein kinase, but...Full Text Available

448

Purpose and regulation of stem cells: a systems-biology view from the Caenorhabditis elegans germ line

Measurements were made of ⁴⁵Ca influx into isolated internodal cells of Chara corallina and also into internodal cells of intact plants. ⁴⁵Ca influx was closely...Full Text Available

1992-10-01

449

Proteasomal chymotrypsin-like peptidase activity is required for essential functions of human monocyte-derived dendritic cells

Stem cells are expected to play a key role in the development and maintenance of organisms, and hold great therapeutic promises. However, a number of questions must be answered to achieve an...Full Text Available

2009-01-01

450

Programmed Cell Death during Pollination-Induced Petal Senescence in Petunia1

The ubiquitin–proteasome pathway is the principal system for extralysosomal protein degradation in eukaryotic cells, and is essential for the regulation and maintenance of basic cellular processes,...Full Text Available

2007-01-01

451

Normal human B lymphocytes and mononuclear cells respond to the mitogenic and cytokine-stimulatory activities of Borrelia burgdorferi and its lipoprotein OspA.

Petal senescence, one type of programmed cell death (PCD) in plants, is a genetically controlled sequence of events comprising its final developmental stage. We characterized the pollination-induced...Full Text Available

2000-04-01

452

Non-myeloablative conditioning with allogeneic hematopoietic cell transplantation for the treatment of high-risk acute lymphoblastic leukemia

Borrelia burgdorferi produces potent cell-activating molecules capable of stimulating polyclonal proliferation and immunoglobulin production by murine B lymphocytes and cytokine production by a variety...Full Text Available

1994-02-01

453

New common variants affecting susceptibility to basal cell carcinoma

BackgroundAllogeneic hematopoietic cell transplantation is a potentially curative treatment for patients with acute lymphoblastic leukemia. However, the majority of older adults...Full Text Available

2011-08-01

454

Near-infrared spectroscopy technique to evaluate the effects of red blood cell transfusion on tissue oxygenation

In a follow-up to our previously reported genome-wide association study of cutaneous basal cell carcinoma (BCC)1, we describe here several new susceptibility...Full Text Available

2009-08-01

455

Mutagenic and cytotoxic activity of doxorubicin and daunorubicin derivatives on prokaryotic and eukaryotic cells.

IntroductionThe aim of this study was to evaluate the effects of red blood cell (RBC) transfusions on muscle tissue oxygenation, oxygen metabolism and microvascular reactivity in...Full Text Available

2009-01-01

456

Miranda as a multidomain adapter linking apically localized Inscuteable and basally localized Staufen and Prospero during asymmetric cell division in Drosophila

The mutagenic and cytotoxic activity of two newly synthesized doxorubicin derivatives and of one daunorubicin derivative were studied in V79 Chinese hamster cells and bacteria (Salmonella typhimurium...Full Text Available

1984-07-01

457

Measurements of accurate x-ray scattering data of protein solutions using small stationary sample cells

Neuroblasts in the developing Drosophila CNS asymmetrically localize the cell fate determinants Numb and Prospero as well as prospero RNA to the basal cortex during...Full Text Available

1998-06-15

458

Management of poor-prognosis testicular germ cell tumors

In this paper, we report a method of precise in situ x-ray scattering measurements on protein solutions using small stationary sample cells. Although reduction in the radiation damage...Full Text Available

2009-01-01

459

Ligands for the peroxisome proliferator-activated receptor-#gamma# have inhibitory effects on growth of human neuroblastoma cells in vitro

Currently, the outcome of patients with intermediate-and poor-risk germ cell tumors at diagnosis is optimized by the use of risk-appropriate chemotherapy and post-chemotherapy surgical resection of...Full Text Available

2010-01-01

460

Laser-assisted solar-cell metallization processing

The thiazolidinedione (TZD) or glitazone class of peroxisome proliferator-activated-#gamma# (PPAR-#gamma#) ligands not only induce adipocyte differentiation and increase insulin sensitivity, but also exert growth inhibitory effects on several carcinoma cell lines in vitro as well as in vivo. In the current study the in vitro effect of four PPAR-#gamma# agonists (ciglitazone, pioglitazone, troglitazone, rosiglitazone) on the cell growth of seven human neuroblastoma cell lines (Kelly, LAN-1, LAN-5, LS, IMR-32, SK-N-SH, SH-SY5Y) was investigated. Growth rates were assessed by a colorimetric XTT-based assay kit. Expression of PPAR-#gamma# protein was examined by immunohistochemistry and Western blot analysis. All glitazones inhibited in vitro growth and viability of the human neuroblastoma cell lines in a dose-dependent manner showing considerable effects only at high concentrations (10 #mu#M and 100 ...

2005-09-15

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Laser-assisted solar cell metallization processing. Quarterly Report, December 1984-February 1985

Laser assisted solar cell metallization processing which is a one step process is examined. The potential advantages of laser disposition techniques for photovoltaic systems are: a high resolution, no photolithography, clean and contamination free, in-situ sintering, and low contact resistance.

1984-03-01

462

K⁺ Channels Are Responsible for an Inwardly Rectifying Current in the Plasma Membrane of Mesophyll Protoplasts of Avena sativa 12

Laser-assisted processing techniques for producing high-quality solar cell metallization patterns are being investigated, developed, and characterized. The tasks comprising these investigations are outlined.

1985-04-01

463

Intra- and extracellular calcium modulates stereocilia stiffness on chick cochlear hair cells.

In whole-cell recording, the conductance of the plasma membrane of protoplasts isolated from mesophyll cells of leaves of oat (Avena sativa) was greater for inward than outward current....Full Text Available

1992-03-01

464

Influence of microenvironment on engraftment of transplanted ?-cells

Segments of the chick basilar papilla were isolated and maintained in culture medium. The sensory hair bundle of individual hair cells was observed with light microscopy and stimulated with a water...Full Text Available

1994-02-01

465

Increased endothelial and vascular smooth muscle cell adhesion on nanostructured titanium and CoCrMo

Pancreatic islet transplantation into the liver provides a possibility to treat selected patients with brittle type 1 diabetes mellitus. However, massive early β-cell death increases the number...Full Text Available

2011-02-01

466

Identification of Human Prostate Cancer Stem Cells by ...

In the body, vascular cells continuously interact with tissues that possess nanostructured surface features due to the presence of proteins (such as collagen and elastin) embedded in the vascular wall....Full Text Available

2006-03-01

467

INDUCIBLE RNAi-MEDIATED GENE SILENCING USING NANOSTRUCTURED GENE DELIVERY ARRAYS

... The basal markers CK5, alpha 6 integrin (CD49f), CD44, and p63 were strongly expressed by the majority of sphere-forming cells. ... p63 p63 PSCA ...

2008-04-01

468

Human Immunodeficiency Virus-1 Inhibition of Immunoamphisomes in Dendritic Cells Impairs Early Innate and Adaptive Immune Responses

RNA interference has become a powerful biological tool over the last decade. In this study, a tetracycline-inducible shRNA vector system was designed for silencing CFP expression and delivered alongside the yfp marker gene into Chinese hamster ovary cells using impalefection on spatially indexed vertically aligned carbon nanofiber arrays (VACNFs). The VACNF architecture provided simultaneous delivery of multiple genes, subsequent adherence and proliferation of interfaced cells, and repeated monitoring of single cells over time. Following impalefection and tetracycline induction, 53.1% 10.4% of impalefected cells were fully silenced by the inducible CFP-silencing shRNA vector. Additionally, efficient CFP-silencing was observed in single cells among a population of cells that remained CFP-expressing. This effective transient expression system enables rapid ...

2008-01-01

469

How cells tiptoe on adhesive surfaces before sticking

SUMMARYDendritic cells (DCs) in mucosal surfaces are early targets for human immunodeficiency virus-1 (HIV-1). DCs mount rapid and robust immune responses upon pathogen encounter....Full Text Available

2010-05-28

470

Guided Cell Migration on Microtextured Substrates with Variable Local Density and Anisotropy

Cell membranes are studded with protrusions that were thoroughly analyzed with electron microscopy. However, the nanometer-scale three-dimensional motions generated by cell membranes to fit the topography of foreign surfaces and initiate adhesion remain poorly understood. Here, we describe the dynamics of surface deformations displayed by monocytic cells bumping against fibronectin-coated surfaces. We observed membrane undulations with typically 5 nm amplitude and 5-10 second lifetime. Cell membranes behaved as independent units of micrometer size. Cells detected the presence of foreign surfaces at 50 nm separation, resulting in time-dependent amplification of membrane undulations. Molecular contact then ensued with apparent cell-membrane separation of 30-40 nm, and this distance steadily decreased during the following tens of seconds. Contact maturation was ...

2008-01-01

471

Genome-Wide Analysis Reveals a Major Role in Cell Fate Maintenance and an Unexpected Role in Endoreduplication for the Drosophila FoxA Gene Fork Head

This work reports the design of and experimentation with a topographically patterned cell culture substrate of variable local density and anisotropy as a facile and efficient platform to guide...Full Text Available

2009-02-06

472

Factors Affecting Daughter Cells' Arrangement during the Early Bacterial Divisions

Transcription factors drive organogenesis, from the initiation of cell fate decisions to the maintenance and implementation of these decisions. The Drosophila embryonic salivary gland...Full Text Available

473

Extraction of immune and inflammatory cells from human lung parenchyma: evaluation of an enzymatic digestion procedure.

On agar plates, daughter cells of Escherichia coli mutually slide and align side-by-side in parallel during the first round of binary fission. This phenomenon has been previously attributed...Full Text Available

474

Establishment of an Immunoglobulin M Antibody-Forming Cell Response Model for Characterizing Immunotoxicity in Primary Human B Cells

The inflammatory and immune cell populations of the human lung parenchyma have not been characterized in detail. This report describes a novel and efficient procedure for their extraction. Histologically...Full Text Available

1986-10-01

475

Epigallocatechin gallate inhibits HBV DNA synthesis in a viral replication - inducible cell line

Rodent models have been extensively utilized to identify putative human immunotoxicants; however, even when immunotoxicity is established, uncertainty remains whether the effects are predictive of human...Full Text Available

2009-12-01

476

Embryonic anti-aging niche

AIM: To analyze the antiviral mechanism of Epigallocatechin gallate (EGCG) against hepatitis B virus (HBV) replication.METHODS: In this research, the HBV-replicating cell line HepG2.117 was used...Full Text Available

2011-03-21

477

Effect of differentiation on specific receptor sites and endocytosis of transferrin in a cell (HT-29) derived from human colic adenocarcinoma

Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. The poor performance of stem cells in an aged...Full Text Available

478

Duration of red blood cell storage is associated with increased incidence of deep vein thrombosis and in hospital mortality in patients with traumatic injuries

The specific receptor sites and the endocytosis of transferrin (Tf) are evidenced in a cell line (HT-29) derived from a human colic adenocarcinoma by means of "1"2"5I radiolabeled Tf. The receptor density is studied in undifferentiated (UD) or differentiated (D) cells with respective doubling times of about 24 hours and 46 hours. The number of binding sites for Tf is 162,000 (K_d = 7.8 nmol/l in ND cells and 68,000 (K_d = 7.40 nmol/l) in D cells. The distribution between the Tf bound to the cell surface and the internalized Tf is investigated by elimination of Tf bound to the surface by an acid wash method. The intracellular cycle of Tf seems to be characterized by a slower kinetics in UD cells. The high density of Tf receptor sites in HT-29 UD cells should allow the detection or the treatment of highly evolutive colic adenocarcinoma by ...

479

Distribution of lead-203 in human peripheral blood in vitro.

IntroductionIn critically ill patients the relationship between the storage age of red blood cells (RBCs) transfused and outcomes are controversial. To determine if duration of RBC...Full Text Available

2009-01-01

480

In-vitro experiments using 203Pb were performed to identify the lead binding components in human peripheral blood. The distribution of lead in plasma, in the red cell membrane, and within the red cell...Full Text Available

1980-02-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

481

Displaced capillary dies

Differentiation of trophoblast stem cells into giant cells is triggered by p57/Kip2 inhibition of CDK1 activity

An asymmetrical shaped capillary die made exclusively of graphite is used to grow silicon ribbon which is capable of being made into solar cells that are more efficient than cells produced from ribbon made using a symmetrically shaped die.

1982-01-01

482

Different doses of bone morphogenetic protein 4 promote the expression of early germ cell-specific gene in bone marrow mesenchymal stem cells

Genome endoreduplication during mammalian development is a rare event for which the mechanism is unknown. It first appears when fibroblast growth factor 4 (FGF4) deprivation induces differentiation...Full Text Available

2008-11-01

483

British Library Electronic Table of Contents (United Kingdom)

Bone morphogenetic protein (BMP)-4 has a crucial role on primordial germ cells (PGCs) development in vivo which can promote stem cell differentiation to PG-like cells. In this study, we investigated the expression of Mvh as one of the specific genes in primordial germ cells after treatment with different doses of BMP4 on bone mesenchymal stem cells (BMSCs)-derived PGCs. Following isolation of BMSCs from male mouse femur and tibia, cells were cultured in medium for 72?h. Passage 4 murine BMSCs were characterized by CD90, CD105, CD34, and CD45 markers and osteo-adipogenic differentiation. Different doses of BMP4 (0, 0.01, 0.1, 1, 5, 25, 50, and 100?ng/ml) were added to BMSCs for PGCs differentiation during 4-days culture. Viability percent, proliferation rates, and expression of Mvh gene wer...

2011-01-01

484

Corticosteroid can alter antigen expression on alveolar macrophages.

Continuous monitoring of receptor-mediated changes in the metabolic rates of living cells.

Normal healthy volunteers underwent broncho-alveolar lavage and the cells obtained were cultured for 24 h and 48 h, either alone or in the presence of the corticosteroid, Budesonide. Cell differentials...Full Text Available

1991-09-01

485

Cloning and Analysis of a Candida albicans Gene That Affects Cell Surface Hydrophobicity

Activation of beta-adrenergic or muscarinic acetylcholine receptors expressed in transfected cells or epidermal growth factor receptors in human keratinocytes produces 15% to 200% changes in cellular...Full Text Available

1990-05-01

486

Chronic inflammatory disease, lymphoid tissue neogenesis and extranodal marginal zone B-cell lymphomas

The opportunistic pathogenic yeast Candida albicans exhibits growth phase-dependent changes in cell surface hydrophobicity, which has been correlated with adhesion to host tissues....Full Text Available

2001-06-01

487

Characterization of Cytokinin and Adenine Transport in Arabidopsis Cell Cultures1[OA

Chronic autoimmune or pathogen-induced immune reactions resulting in lymphoid neogenesis are associated with development of malignant lymphomas, mostly extranodal marginal zone B-cell lymphomas (MZBCLs)....Full Text Available

2009-08-01

488

Carcinoma in situ of contralateral testis in patients with testicular germ cell cancer: study of 27 cases in 500 patients.

Cytokinins are distributed through the vascular system and trigger responses of target cells via receptor-mediated signal transduction. Perception and transduction of the signal can occur at the plasma...Full Text Available

2008-12-01

489

Blood conservation strategies to reduce the need for red blood cell transfusion in critically ill patients

Carcinoma in situ in the contralateral testis was diagnosed in 27 of 500 patients (5.4%) with unilateral testicular germ cell cancer. Eight of the 27 patients received intensive chemotherapy for spread...Full Text Available

1986-11-29

490

Bacterial Particle Endocytosis by Epithelial Cells Is Selective and Enhanced by Tumor Necrosis Factor Receptor Ligands?

Anemia commonly affects critically ill patients. The causes are multifactorial and include acute blood loss, blood loss from diagnostic testing and blunted red blood cell production. Blood transfusions...Full Text Available

2008-01-01

491

Association between length of storage of red blood cell units and outcome of critically ill children: a prospective observational study

Bacterial pathogens use virulence strategies to invade epithelial barriers, but active processes of epithelial cells may also contribute to the endocytosis of microbial particles. To focus on the latter,...Full Text Available

2009-03-01

492

Annual Report for Gravity Collection Lysimeter Monitoring Plan - ERDF Cells 5 and 6

IntroductionTransfusion is a common treatment in pediatric intensive care units (PICUs). Studies in adults suggest that prolonged storage of red blood cell units is associated with...Full Text Available

2010-01-01

493

Agonist-dependent up-regulation of thyrotrophin-releasing hormone receptor protein.

The data and analyses contained in this report reflect the initial characterization of construction and consolidation water in Cells 5 and 6 lysimeters. Therefore, the scope of this report will be to establish constituent levels and document dewatering activities completed to date.

2006-12-19

494

Addressing the Question of the Effect of RBC Storage on Clinical Outcomes: The Red Cell Storage Duration Study (RECESS) (Section 7)

To study the effect of agonist on the TRH (thyrotrophin-releasing hormone) receptor protein, an epitope-tagged receptor was stably expressed in HEK-293 cells (human embryonic kidney 293 cells) and receptor...Full Text Available

2004-06-15

495

Abscisic Acid Increases Terrestrial Plant Cell Resistance to Hydrostatic Pressure 1

The question of whether storage of red blood cells (RBCs) alters their capacity to deliver oxygen and affects patient outcomes remains in a state of clinical equipoise. Studies of the changes...Full Text Available

2010-08-01

496

A versatile and potentially general approach to the targeting of specific cell types by retroviruses: Application to the infection of human cells by means of major histocompatibility complex class I and class II antigens by mouse ecotropic murine leukemia virus-derived viruses

Cells of the terrestrial plant species bromegrass (Bromus inermis L.) are not naturally adapted to withstand the hydrostatic pressures encountered in aquatic environments. However,...Full Text Available

1992-02-01

497

A proteomic study of cMyc improvement of CHO culture

A technique for delivering genes carried by recombinant retroviruses into specific cell types could have numerous applications in oncology, developmental biology, and gene therapy. As a first step toward this remote goal the authors designed a procedure allowing in vitro cell targeting by retroviruses. Biotinylated antibodies against the viral envelope protein on one side, and against specific cell membrane markers on the other side, were bridged by streptavidin and used to link the virus to the host. The method was successfully used to infect human cells with ecotropic murine retroviruses by means of major histocompatibility complex class I and class II antigens and appears easily adaptable to other cell, membrane markers. Moreover, the sequential protocol they design, although allowing infection of human cells, requires less stringent safety constraints than ...

1989-12-01

498

A global role for KLF1 in erythropoiesis revealed by ChIP-seq in primary erythroid cells

BackgroundThe biopharmaceutical industry requires cell lines to have an optimal proliferation rate and a high integral viable cell number resulting in a maximum volumetric recombinant...Full Text Available

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