yeast efficiently secreting: Topics by WorldWideScience.org

Overexpression of human virus surface glycoprotein precursors induces cytosolic unfolded protein response in Saccharomyces cerevisiae

Yeast arginyl-tRNA synthetase recognizes the non-modified wild-type transcripts derived from both yeast tRNA(Arg) and tRNA(Asp) with equal efficiency. It discriminates its cognate natural substrate,...Full Text Available

1996-09-16

4

Efficient quantum secure communication scheme with one-time pad

BackgroundThe expression of human virus surface proteins, as well as other mammalian glycoproteins, is much more efficient in cells of higher eukaryotes rather than yeasts. The limitations...Full Text Available

5

Communication and Round Efficient Information Checking Protocol

In this paper, we proposed a novel quantum secure direct communication scheme with one-time pad in stabilizer formalism. Based on the reuse of qubit sequence, an efficient secure communication of secret messages without first producing a shared secret key can be achieved. One hence may find that the amount of private key needed for quantum communication is smaller than that in the general case. Therefore, the present protocol which is feasible with the present-day techniques may be applied to quantum communication with short-length encoding.

2009-05-01

6

Hybridization with synthetic oligonucleotides

In this paper, we present a very important primitive called Information Checking Protocol (ICP) which plays an important role in constructing statistical Verifiable Secret Sharing (VSS) and Weak Secret Sharing (WSS) protocols. Informally, ICP is a tool for authenticating messages in the presence of computationally unbounded corrupted parties. Here we extend the basic bare-bone definition of ICP, introduced by Rabin et al. and then present an ICP that attains the best communication complexity and round complexity among all the existing ICPs in the literature. We also show that our ICP satisfies several interesting properties such as linearity property which is an important requirement in many applications of ICP. Though not presented in this paper, we can design communication and round efficient statistical (i.e involves negligible error probability in computation) VSS and Multiparty Computation (MPC) protocol using our new ...

2010-01-01

7

Enhanced Degradation of an Endocrine-Disrupting Chemical, Butyl Benzyl Phthalate, by Fusarium oxysporum f. sp. pisi Cutinase

Procedures are described for the use of synthetic oligonucleotides for Southern blot experiments and gene bank screening, and the effect of various mismatches on the efficiency of hybridization is demonstrated. The following topics are discussed: sensitivity vs. specificity, hybridization of a 12-mer to the lambda endolysin gene; hybridization of oligonucleotide probes to the E. coli lac operator; hybridization of synthetic probes to the CYC1 gene of yeast; and cloning eucaryotic genes. (HLW)

1978-01-01

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The enhancement of three-party simultaneous quantum secure direct communication scheme with EPR pairs

Compared to yeast esterase, fungal cutinase degraded butyl benzyl phthalate (BBP) far more efficiently; i.e., almost 60% of the BBP disappeared within 7.5 h. Also, the final chemical composition significantly...Full Text Available

2002-09-01

9

Secrets in the Ancient Goatskins: X-Rays Reveal Archimedes' Oldest Writings

Recently, Wang et al. proposed a three-party simultaneous quantum secure direct communication (3P-SQSDC) scheme with EPR pairs, which enables three involved parties to exchange their secret messages simultaneously by using an EPR pair. This work proposed an enhancement on Wang et al.'s scheme. With the enhancement, the communications in the improved 3P-SQSDC can be paralleled and thus improves the protocol efficiency.

2011-01-01

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ScienceCinema

...english ...

11

An efficient quantum secure direct communication scheme with authentication

Effects of optisol-GS incubation on the profile of corneal synthesis and secretion- partial characterization of the secreted p{sub 25} fraction

In this paper an efficient quantum secure direct communication (QSDC) scheme with authentication is presented, which is based on quantum entanglement and polarized single photons. The present protocol uses Einstein-Podolsky-Rosen (EPR) pairs and polarized single photons in batches. A particle of the EPR pairs is retained in the sender's station, and the other is transmitted forth and back between the sender and the receiver, similar to the ``ping-pong'' QSDC protocol. According to the shared information beforehand, these two kinds of quantum states are mixed and then transmitted via a quantum channel. The EPR pairs are used to transmit secret messages and the polarized single photons used for authentication and eavesdropping check. Consequently, because of the dual contributions of the polarized single photons, no classical information is needed. The intrinsic efficiency and total efficiency are both 1 ...

2007-07-01

12

Latvian scientists research into chemical uses of timber

No abstract prepared.

2007-07-01

13

Survival of Genetically Modified and Self-Cloned Strains of Commercial Baker's Yeast in Simulated Natural Environments: Environmental Risk Assessment

Scientists of the Institute of Wood Chemistry of the Latvian Academy of Sciences have developed two highly efficient processes for producing furfural, a feedstock for varnishes, synthetic resins and plastics. It is made of production wastes, including branches and small-dimension timber. By one process, the raw material is chipped, treated first with diluted sulphuric acid and then with steam heated to 250 degrees C. The other uses concentrated sulphuric acid as a catalyst. Besides furfural, this process also yields sugar solutions used in alcohol and nutrient yeast production.

1982-03-20

14

Comparison of the Survival and Metabolic Activity of Psychrophilic and Mesophilic Yeasts Subjected to Freeze-Thaw Stress

Although genetic engineering techniques for baker's yeast might improve the yeast's fermentation characteristics, the lack of scientific data on the survival of such strains in natural environments...Full Text Available

2005-11-01

15

Global Transcriptional Responses of Fission Yeast to Environmental Stress

A mesophilic yeast, Candida utilis, and a psychrophilic yeast, Leucosporidium stokesii, were subjected to freeze-thaw cycling over the range 25 to -60 C. Viability...Full Text Available

1975-06-01

16

Apoptosis at Inflection Point in Liquid Culture of Budding Yeasts

We explored transcriptional responses of the fission yeast Schizosaccharomyces pombe to various environmental stresses. DNA microarrays were used to characterize changes in expression...Full Text Available

2003-01-01

17

Bootstrapping Key Pre-Distribution: Secure, Scalable and User-Friendly Initialization of Sensor Nodes

Budding yeasts are highly suitable for aging studies, because the number of bud scars (stage) proportionally correlates with age. Its maximum stages are known ...Full Text Available

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Alteration of the enterohepatic recirculation of bile acids in rats after exposure to ionizing radiation

To establish secure (point-to-point and/or broadcast) communication channels among the nodes of a wireless sensor network is a fundamental task. To this end, a plethora of (socalled) key pre-distribution schemes have been proposed in the past. All these schemes, however, rely on shared secret(s), which are assumed to be somehow pre-loaded onto the sensor nodes. In this paper, we propose a novel method for secure initialization of sensor nodes based on a visual out-of-band channel. Using the proposed method, the administrator of a sensor network can distribute keys onto the sensor nodes, necessary to bootstrap key pre-distribution. Our secure initialization method requires only a little extra cost, is efficient and scalable with respect to the number of sensor nodes. Moreover, based on a usability study that we conducted, the method turns out to be quite user-friendly and easy to use by naive human users.

2008-01-01

19

Mycobacterium tuberculosis Chaperonin 10 Is Secreted in the Macrophage Phagosome: Is Secretion Due to Dissociation and Adoption of a Partially Helical Structure at the Membrane?

The aim of this work was to study acute alterations of the enterohepatic recirculation (EHR) of bile acids 3 days after an 8-Gy radiation exposure in vivo in the rat by a washout technique. Using this technique in association with HPLC analysis, the EHR of the major individual bile acids was determined in control and irradiated animals. Ex vivo ileal taurocholate absorption was also studied in Ussing chambers. Major hepatic enzyme activities involved in bile acid synthesis were also measured. Measurements of bile acid intestinal content and intestinal absorption efficiency calculation from washout showed reduced intestinal absorption with significant differences from one bile acid to another: absorption of taurocholate and tauromuricholate was decreased, whereas absorption of the more hydrophobic taurochenodeoxycholate was increased, suggesting that intestinal passive diffusion was enhanced, whereas ileal active transport might be reduced. Basal hepatic ...

2004-02-01

20

The Secretion of ?-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro

To confirm that Mycobacterium tuberculosis chaperonin 10 (Cpn10) is secreted outside the live bacillus, infected macrophages were examined by electron microscopy. This revealed that...Full Text Available

2003-07-01

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Promoter Structure Which Affects on the Expression of Yeast MGMT Gene

Prothoracic glands of tobacco hornworm larvae cultured in vitro secrete into the culture medium a substance which was active in ecdysone bioassays and determined to be ecdysone-like...Full Text Available

1974-03-01

22

Effect of Zinc-Enriched Yeast Supplementation on Serum Zinc and Testosterone Concentrations in Ethanol Feeding Rats

Full Text Available

1997-01-01

23

Application of Quantitative Real-Time PCR for Enumeration of Total Bacterial, Archaeal, and Yeast Populations in Kimchi

Full Text Available

2008-07-01

24

Parietal endoderm secreted S100A4 promotes early cardiomyogenesis in embryoid bodies

Full Text Available

2009-12-01

25

DEFF Research Database (Denmark)

Udgivelsesdato: 2006-May-5

2006-01-01

26

Naltrexone Inhibits Catecholamine Secretion Evoked by Nicotinic Receptor Stimulation in the Perfused Rat Adrenal Medulla

Fermat's last theorem unlocking the secret of an ancient mathematical problem

Full Text Available

2005-08-01

27

3-Methyl-3-deazaadenine, a stable isostere of N3-methyl-adenine, is efficiently bypassed by replication in vivo and by transcription in vitro.

Fermat's last theorem

1997-01-01

28

The type III secretion system is involved in Escherichia coli K1 interactions with Acanthamoeba

The goal of the present work was to determine the impact of N3-methyladenine (3-mA), an important lesion generated by many environmental agents and anticancer drugs, on in vivo DNA replication and in vitro RNA transcription. Due to 3-mA chemical instability, the stable isostere 3-methyl-3-deazaadenine (3-m-c(3)A) was site specifically positioned into an oligodeoxynucleotide. The oligomer was, then incorporated into a vector system that is rapidly converted to ssDNA inside yeast cells and requires DNA replication opposite the lesion for plasmid clonal selection. For control purposes, an adenine or a stable apurinic/apyrimidinic (AP)-lesion was placed at the same site. The presence of each lesion in the oligonucleotide was confirmed by MALDI-TOF analysis. Plasmids were then transfected into yeast cells. While the AP-site dramatically reduced plasmid replication in all strains, the 3-m-c(3)A had a slight effect in the rad30 background which ...

2011-06-14

29

British Library Electronic Table of Contents (United Kingdom)

The type III secretion system among Gram-negative bacteria is known to deliver effectors into host cell to interfere with host cellular processes. The type III secretion system in Yersina, Pseudomonas and Enterohemorrhagic Escherichia coli have been well documented to be involved in the bacterial pathogenicity. The existence of type III secretion system has been demonstrated in neuropathogenic E. coli K1 strains. Here, it is observed that the deletion mutant of type III secretion system in E. coli strain EC10 exhibited defects in the invasion and intracellular survival in Acanthamoeba castellanii (a keratitis isolate) compared to its parent strain. Next, it was determined whether type III secretion system plays a role in E. coli K1 survival inside Acanthamoeba during the encystment process...

2011-01-01

30

Modular coherence of protein dynamics in yeast cell polarity system

METABOLIC REGULATION OF ADENOSINE TRIPHOSPHATE SULFURYLASE IN YEAST

In this study, we investigated on a systems level how complex protein interactions underlying cell polarity in yeast determine the dynamic association of proteins with the polar cortical domain (PCD)...Full Text Available

2011-05-03

31

In vivo expression and mitochondrial targeting of yeast apoiso-1-cytochrome c fusion proteins.

de Vito, Peter C. (Princeton University, Princeton, N.J.), and Jacques Dreyfuss. Metabolic regulation of adenosine triphosphate sulfurylase in yeast. J. Bacteriol. 88:1341–1348....Full Text Available

1964-11-01

32

Heavy metals alter the electrokinetic properties of bacteria, yeasts, and clay minerals.

To define the import pathway for apoiso-1-cytochrome c in vivo, the coding region for bacterial chloramphenicol acetyltransferase (CAT) or yeast copper metallothionein (CuMT) was fused to the carboxy...Full Text Available

1990-11-01

33

Evaluation of Acyl Coenzyme A Oxidase (Aox) Isozyme Function in the n-Alkane-Assimilating Yeast Yarrowia lipolytica

The electrokinetic patterns of four bacterial species (Bacillus subtilis, Bacillus megaterium, Pseudomonas aeruginosa, and Agrobacterium radiobacter), two yeasts (Saccharomyces cerevisiae and Candida...Full Text Available

1992-05-01

34

Antagonistic Gcn5-Hda1 interactions revealed by mutations to the Anaphase Promoting Complex in yeast

We have identified five acyl coenzyme A (CoA) oxidase isozymes (Aox1 through Aox5) in the n-alkane-assimilating yeast Yarrowia lipolytica, encoded by the POX1...Full Text Available

1999-09-01

35

A Novel Function of the DNA Repair Gene rhp6 in Mating-Type Silencing by Chromatin Remodeling in Fission Yeast

BackgroundHistone post-translational modifications are critical for gene expression and cell viability. A broad spectrum of histone lysine residues have been identified in yeast...Full Text Available

36

DNA double strand breakage after UV irradiation of yeast cells; DNA-Doppelstrangbrueche nach UV-Bestrahlung in Hefezellen

Recent studies have indicated that the DNA replication machinery is coupled to silencing of mating-type loci in the budding yeast Saccharomyces cerevisiae, and a similar silencing mechanism...Full Text Available

1998-09-01

37

Cholera toxin binding sites in yeast triggers biochemical pathway

No abstract prepared.

2000-07-01

38

Characterization of commercially available selenized yeast food supplements

... e Biologia Molecular (SBBq), Sao Paulo, SP (Brazil) 217 p. APPLIED LIFE

1998-05-23

39

Effect of UV radiation on the killer phenotype in the wine yeast-saccharomycetes and spontaneous variation of this character

... Rome (Italy) Istituto Superiore di Sanita (Italy) 207 p. ENVIRONMENTAL

2009-04-01

40

Ethanol production and a case study of ethanol produced from sweet sorghum stalks via solid state fermentation

Spontaneous and ultraviolet-induced changeabilities of wine yeasts from the killer state to sensitive one have been studied. Observed often spontaneous changes of killer and neutral phenotypes under laboratory store conditions as well as high mutation frequency of genetic elements responsible for the killer indication on ultraviolet irradiation testify that often encounterability in nature and in the production of sensitive yeasts is attributed to high frequency of mutation changes of the killer and neutral phenotypes to the sensitive state.

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Unconventional Secretion of AcbA in Dictyostelium discoideum through a Vesicular Intermediate?

Ethanol has excellent fuel properties, such as high octane, high heat of vaporization and low photochemical reactivity in the atmosphere. It is less volatile than gasoline and there is lower smog formation from evaporative emissions of pure ethanol compared to gasoline. As such, ethanol has emerged as an important alternative energy source that is sustainable, efficient, cost effective, convenient and safe. In 2006, global production of ethanol reached 13.5 billion gallons, up from 12.1 billion gallons in 2005. However, in light of the current debate of food versus fuel, the industry must shift to non-food feedstocks. This paper described an emerging technology to cost-effectively produce ethanol from sweet sorghum stalks, the most promising alternative feedstock to corn, via solid state fermentation (SSF). Experiments of advanced solid state fermentation (ASSF) for ethanol production from sweet sorghum by Saccharomyces cerevisiae were conducted in laboratory and ...

2008-07-01

42

Type IVB Secretion Systems of Legionella and Other Gram-Negative Bacteria

The acyl coenzyme A (CoA) binding protein AcbA is secreted unconventionally and processed into spore differentiation factor 2 (SDF-2), a peptide that coordinates sporulation in Dictyostelium...Full Text Available

2010-07-01

43

Self-Chaperoning of the Type III Secretion System needle tip proteins IpaD and BipD

Type IV secretion systems (T4SSs) play a central role in the pathogenicity of many important pathogens, including Agrobacterium tumefaciens, Helicobacter pylori, and...Full Text Available

44

Regulation of hyaluronan secretion into rabbit synovial joints in vivo by protein kinase C

Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid...Full Text Available

2007-02-09

45

Membrane alteration is necessary but not sufficient for effective glutamate secretion in Corynebacterium glutamicum.

Hyaluronan (HA) is important for joint cavitation, lubrication, volume regulation and synovial fluid drainage but little is known about the regulation of joint HA synthesis/secretion in vivo....Full Text Available

2003-07-15

46

Lethal protein produced in response to competition between sibling bacterial colonies

We showed recently that secretion of glutamate in biotin-limited cells of Corynebacterium glutamicum is mediated by carrier systems in the plasma membrane (C. Hoischen and R. Krämer, Arch. Microbiol....Full Text Available

1990-06-01

47

Controlled Bidirectional Quantum Direct Communication by Using a GHZ State

Sibling Paenibacillus dendritiformis bacterial colonies grown on low-nutrient agar medium mutually inhibit growth through secretion of a lethal factor. Analysis of secretions reveals...Full Text Available

2010-04-06

48

The hrpK Operon of Pseudomonas syringae pv. tomato DC3000 Encodes Two Proteins Secreted by the Type III (Hrp) Protein Secretion System: HopB1 and HrpK, a Putative Type III Translocator

A controlled bidirectional quantum secret direct communication scheme is proposed by using a Greenberger-Horne-Zeilinger (GHZ) state. In the scheme, two users can exchange their secret messages simultaneously with a set of devices under the control of a third party. The security of the scheme is analysed and confirmed.

2006-07-01

49

Mapping of TH1 helper T-cell epitopes on major secreted mycobacterial antigen 85A in mice infected with live Mycobacterium bovis BCG.

Pseudomonas syringae is a gram-negative bacterial plant pathogen that is dependent on a type III protein secretion system (TTSS) and the effector proteins it translocates into plant...Full Text Available

2005-01-01

50

Human rib bone marrow mononuclear cells spontaneously synthesize and secrete IgE in vitro.

TH1 cytokine secretion was examined in response to synthetic peptides of the 85A component of the major secreted, fibronectin-binding antigen 85 complex from Mycobacterium tuberculosis in seven different...Full Text Available

1994-02-01

51

Quantum secure direct communication by EPR pairs and entanglement swapping

We have examined spontaneous secretion of IgE by human rib bone marrow mononuclear cells (MNC). Bone marrow MNC from nine out of 12 rib specimens synthesized and secreted substantial amounts of IgE...Full Text Available

1991-01-01

52

Entanglement-secured single-qubit quantum secret-sharing

We present a quantum secure direct communication scheme achieved by swapping quantum entanglement. In this scheme a set of ordered Einstein-Podolsky-Rosen (EPR) pairs is used as a quantum information channel for sending secret messages directly. After insuring the safety of the quantum channel, the sender Alice encodes the secret messages directly by applying a series local operations on her particle sequences according to their stipulation. Using three EPR pairs, three bits of secret classical information can be faithfully transmitted from Alice to remote Bob without revealing any information to a potential eavesdropper. By both Alice and Bob's GHZ state measurement results, Bob is able to read out the encoded secret messages directly. The protocol is completely secure if perfect quantum channel is used, because there is not a transmission of the qubits carrying the secret message ...

2004-03-01

53

Removal of lead from solution using non-living residual brewery yeast

In single-qubit quantum secret sharing, a secret is shared between N parties via manipulation and measurement of one qubit at a time. Each qubit is sent to all N parties in sequence; the secret is encoded in the first participant's preparation of the qubit state and the subsequent participants' choices of state rotation or measurement basis. We present a protocol for single-qubit quantum secret sharing using polarization entanglement of photon pairs produced in type-I spontaneous parametric downconversion. We investigate the protocol's security against eavesdropping attack under common experimental conditions: a lossy channel for photon transmission, and imperfect preparation of the initial qubit state. A protocol which exploits entanglement between photons, rather than simply polarization correlation, is more robustly secure. We implement the entanglement-based secret-sharing ...

2011-01-01

54

Genetical approach to oxygen toxicity. [Saccharomyces cerevisiae

A number of preparations of residual non-living brewery yeast were examined for their ability to remove lead from solution. Those preparations included washed and un-washed intact yeast and washed and un-washed homogenates of the yeast cells. Using biosorption isotherm analysis it was found that the washed and un-washed preparations of intact, non-living yeast exhibited maximum biosorption capacities for lead of 127 and 99 mg/g dry weight biomass, respectively. The washed and un-washed cell homogenates exhibited maximum biosorption capacities of 38 and 139 mg lead/g dry weight biomass, respectively. Since it had previously been shown that these preparations of biomass were capable of removing uranium from solution by combined biosorption and precipitation processes, it was decided to examine removal of lead from solution using a form of equilibrium dialysis in which the biomass was retained within a ...

1998-10-01

55

Sirtuins, Bioageing, and Cancer

The role of intermediate products of dioxygen reduction in cytotoxic effects ascribed to oxygen molecules was studied in vivo using various yeast mutants with changed response to oxygen stress. It has been documented that superoxide radical exerts its deleterious effects on yeast cells directly and the role of other oxygen species derived from it is hardly detectable. Yeast Saccharomyces cerevisiae, however, cannot be considered as a typical eukaryotic organism due to its inability of synthesizing polyunsaturated fatty acids (pufa). These fatty acids are known as main target molecules during oxidative stress and their peroxidation leads to cytotoxic effects. As fatty acid content could be easily manipulated in yeast, this organism was used to evaluate the contribution of pufa peroxidation process to the cytotoxic effects of oxygen. Results obtained show, that yeast cells containing ...

1986-01-01

56

Iodination of glyceraldehyde 3-phosphate dehydrogenase

The Sirtuins are a family of orthologues of yeast Sir2 found in a wide range of organisms from bacteria to man. They display a high degree of conservation between species, in both sequence and function,...Full Text Available

57

Effect of Photosynthetic Bacterial Addition to Chlorella or ${\\omega}-Yeast$ on Growth of Rotifer, Brachionus plicatilis, and its Dietary Value for Flounder, Paralichthys olivaceus, Larvae

1. A high degree of homology in the positions of tyrosine residues in glyceraldehyde 3-phosphate dehydrogenase from lobster and pig muscle, and from yeast, prompted an examination of the reactivity...Full Text Available

1970-09-01

58

A Simultaneous Quantum Secure Direct Communication Scheme between the Central Party and Other M Parties

Full Text Available

2000-03-01

59

Capacity of a Simultaneous Quantum Secure Direct Communication Scheme between the Central Party and Other M Parties

We propose a simultaneous quantum secure direct communication scheme between one party and other three parties via four-particle GHZ states and swapping quantum entanglement. In the scheme, three spatially separated senders, Alice, Bob and Charlie, transmit their secret messages to a remote receiver Diana by performing a series of local operations on their respective particles according to the quadripartite stipulation. From Alice, Bob, Charlie and Diana's Bell measurement results, Diana can infer the secret messages. If a perfect quantum channel is used, the secret messages are faithfully transmitted from Alice, Bob and Charlie to Diana via initially shared pairs of four-particle GHZ states without revealing any information to a potential eavesdropper. As there is no transmission of the qubits carrying the secret message in the public channel, it is completely secure for the direct ...

2005-10-01

60

Technical Report for the Period 1 January 1963 to 30 June ...

We analyse the capacity of a simultaneous quantum secure direct communication scheme between the central party and other M parties via M+1-particle GHZ states and swapping quantum entanglement. It is shown that the encoding scheme should be secret if other M parties wants to transmit M+1 bit classical messages to the centre party secretly. However, when the encoding scheme is announced publicly, we prove that the capacity of the scheme in transmitting the secret messages is 2 bits, no matter how large M is.

2006-10-01

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61

Standard of mineralocorticoid secretion in the Cushing Syndrome: evidences of inhibition of the aldosterone path synthesis in the glomerulus zone

... Combustible ..... 24 - S-CRET/DISCREET Page 33. SECRET/D ISCREET Combustible Cartridge Cases CONFIDENTIAL ...

1963-08-23

62

New Developments in the Use of Histamine and Histamine Receptors

Portuguese 1984. p. 146. Brazil Czepielewski, MA Kater, CE Biglieri, EG Escola

1984-10-27

63

Multiparty controlled quantum secure direct communication using Greenberger Horne Zeilinger state

Histamine and the histamine receptors are important regulators of a plethora of biological processes, including immediate hypersensitivity reactions and acid secretion in the stomach. In these...Full Text Available

2011-04-01

64

Cancer cells cause vascular endothelial cell (vEC) retraction via 12(S)HETE secretion; the possible role of cancer cell derived microparticle.

Based on the idea of dense coding of three-photon entangled state and qubit transmission in blocks, we present a multiparty controlled quantum secret direct communication scheme by using Greenberger Horne Zeilinger state. In the present scheme, the sender transmits three bits of secret message to the receiver directly and the secret message can only be recovered by the receiver under the permission of all the controllers. All three-photon entangled states are used to transmit the secret message except those chosen for eavesdropping check and the present scheme has a high source capacity because Greenberger Horne Zeilinger state forms a large Hilbert space.

2006-10-01

65

Alpha-toxin of Staphylococcus aureus.

PMID:17103063

2006-11-12

66

The Functionally Conserved Nucleoporins Nup124p from Fission Yeast and the Human Nup153 Mediate Nuclear Import and Activity of the Tf1 Retrotransposon and HIV-1 VprV?

Alpha-toxin, the major cytotoxic agent elaborated by Staphylococcus aureus, was the first bacterial exotoxin to be identified as a pore former. The protein is secreted as a single-chain, water-soluble...Full Text Available

1991-12-01

67

Morphological Instabilities in a growing Yeast Colony Experiment and Theory

We report that the fission yeast nucleoporin Nup124p is required for the nuclear import of both, retrotransposon Tf1-Gag as well as the retroviral HIV-1 Vpr. Failure to import Tf1-Gag into the nucleus...Full Text Available

2005-04-01

68

Kes1p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis.

We study the growth of colonies of the yeast Pichia membranaefaciens on agarose film. The growth conditions are controlled in a setup where nutrients are supplied through an agarose film suspended over a solution of nutrients. As the thickness of the agarose film is varied, the morphology of the front of the colony changes. The growth of the front is modeled by coupling it to a diffusive field of inhibitory metabolites. Qualitative agreement with experiments suggests that such a coupling is responsible for the observed instability of the front.

1997-01-01

69

The yeast phosphatidylinositol transfer protein (Sec14p) is required for biogenesis of Golgi-derived transport vesicles and cell viability, and this essential Sec14p requirement is abrogated by inactivation...Full Text Available

1996-12-02

70

DNA repair genes

Amino acid sequences that determine the nuclear localization of yeast histone 2B.

Fission yeast S. pombe is assumed to be a good model for cloning of human DNA repair genes, because human gene is normally expressed in S. pombe and has a very similar protein sequence to yeast protein. We have tried to elucidate the DNA repair mechanisms of S. pombe as a model system for those of mammals. (J.P.N.)

1995-12-01

71

A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism.

Histone-beta-galactosidase protein fusions were used to identify the domain of yeast histone 2B, which targets this protein to the nucleus. Amino acids 28 to 33 in H2B were required for nuclear localization...Full Text Available

1987-11-01

72

A New Metal-Binding Site for Yeast Phosphoglycerate Kinase as Determined by the Use of a Metal-ATP Analog

Transcription activation of sulfur metabolism in yeast is dependent on two DNA binding factors, the centromere binding factor 1 (Cbf1) and Met4. While the role of Met4 was clearly established by showing...Full Text Available

1996-05-15

73

Pseudomonas aeruginosa tssC1 Links Type VI Secretion and Biofilm-Specific Antibiotic Resistance.

Suicide substrate β, γ-bidentate Rh(III)ATP (RhATP) was used to map the metal ion-binding site in yeast phosphoglycerate kinase (PGK). Cleavage of the RhATP-inactivated enzyme with...Full Text Available

1997-02-01

74

Nonlinear analysis and prediction of pulsatile hormone secretion

Biofilm-specific antibiotic resistance is influenced by multiple factors. We demonstrated that Pseudomonas aeruginosa tssC1, a gene implicated in type VI secretion (T6S), is important for resistance of biofilms to a subset of antibiotics. We showed that tssC1 expression is induced in biofilms and confirmed that tssC1 is required for T6S. PMID:21784934

2011-07-22

75

Enhanced glycemic responsiveness to epinephrine in insulin-dependent diabetes mellitus is the result of the inability to secrete insulin. Augmented insulin secretion normally limits the glycemic, but not the lipolytic or ketogenic, response to epinephrine in humans.

Pulsatile hormone secretion is observed in almost every hormonal system. The frequency of episodic hormone release ranges from approximately 10 to 100 pulses in 24 hours. This temporal mode of secretion is an important feature of intercellular information transfer in addition to a dose-response dependent regulation. It has been demonstrated in a number of experiments that changes in the temporal pattern of pulsatile hormone secretion specifically regulate cellular and organ function and structure. Recent evidence links osteoporosis, a disease characterized by loss of bone mass and structure, to changes in the dynamics of pulsatile parathyroid hormone (PTH) secretion. In our study we applied nonlinear and linear time series prediction to characterize the secretory dynamics of PTH in both healthy human subjects and patients with osteoporosis. Osteoporotic patients appear to lack periods of high ...

1996-06-01

76

Effects of Temperature on H⁺ Secretion and Uptake by Excised Flexor Cells during Dark-Induced Closure of Samanea Leaflets ¹

To determine if the enhanced glycemic response to epinephrine in patients with insulin-dependent diabetes mellitus (IDDM) is the result of increased adrenergic sensitivity per se, increased glucagon...Full Text Available

1985-06-01

77

Biochemical and Structural Characterization of the Secreted Chorismate Mutase (Rv1885c) from Mycobacterium tuberculosis H₃₇R_v: an *AroQ Enzyme Not Regulated by the Aromatic Amino Acids

Previous studies reveal that dark-induced closure of Samanea leaflets is accompanied by H⁺ secretion from flexor motor cells. We now report that flexor tissue excised in...Full Text Available

1987-11-01

78

Deterministic secure direct communication using GHZ states and swapping quantum entanglement

The gene Rv1885c from the genome of Mycobacterium tuberculosis H₃₇R_v encodes a monofunctional and secreted chorismate mutase (*MtCM) with a 33-amino-acid...Full Text Available

2006-12-01

79

Comparison of the effects of enteral feeding with continuous and intermittent parenteral nutrition on hepatic triglyceride secretion in human beings

We present a deterministic secure direct communication scheme via entanglement swapping, where a set of ordered maximally entangled three-particle states (GHZ states), initially shared by three spatially separated parties, Alice, Bob and Charlie, functions as a quantum information channel. After ensuring the safety of the quantum channel, Alice and Bob apply a series of local operations on their respective particles according to the tripartite stipulation and the secret message they both want to send to Charlie. By three of Alice, Bob and Charlie's Bell measurement results, Charlie is able to infer the secret messages directly. The secret messages are faithfully transmitted from Alice and Bob to Charlie via initially shared pairs of GHZ states without revealing any information to a potential eavesdropper. Since there is no transmission of the qubits carrying the secret message between any two of them in ...

2005-06-24

80

Molecular evolution and characterization of fungal indoleamine 2,3-dioxygenases.

Plasma triglyceride turnover was measured during steady-state conditions in 22 postoperative patients. Nine had received nutritional support with an enteral regimen, seven had received an equivalent regimen as continuous parenteral nutrition, and six received the same parenteral regimen as a cyclical infusion. After 5 days of nutritional support, each patient received an intravenous bolus of tritiated glycerol. Plasma radiolabeled triglyceride content was measured during the subsequent 24 hours. The data were analyzed by means of a simple deterministic model of plasma triglyceride kinetics and compared with the results obtained by stochastic analysis. The rates of hepatic triglyceride secretion obtained by deterministic analysis were higher than those obtained by the stochastic approach. However, the mode of delivery of the nutritional regimen did not affect the rate of hepatic triglyceride secretion regardless of the method of analysis. The ...

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81

Functions of mammalian Cdc7 kinase in initiation/monitoring of DNA replication and development

Indoleamine 2,3-dioxygenase (IDO) and tryptophan 2,3-dioxygenase (TDO) are tryptophan-degrading enzymes. Mammalian IDO expression is induced by cytokines and has antimicrobial and immunomodulatory effects. A major role of mammalian TDO is to supply nicotinamide adenine dinucleotide (NAD(+)). In fungi, the IDO homologue is thought to be expressed constitutively and supply NAD(+), as TDO is absent from their genomes. Here, we reveal the distribution of IDO genes among fungal species and characterize their enzymatic activity. The yeast, Saccharomyces cerevisiae has only one IDO gene, whereas the koji-mold, Aspergillus oryzae has two genes, IDO? and IDO?. The A. oryzae IDO? showed more similar enzymatic properties to those of S. cerevisiae IDO than IDO?, suggesting that the A. oryzae IDO? is a functional homologue of the S. cerevisiae IDO. From the IDO? gene, two isoforms, IDO? and IDO?(+) could be generated by alternative splicing. The latter contained a 17 amino ...

2010-12-18

82

Biological assessments of a mixture of endocrine disruptors at environmentally relevant concentrations in water following UV/H{sub 2}O{sub 2} oxidation

Cdc7 kinase plays an essential role in firing of replication origins by phosphorylating components of the replication complexes. Cdc7 kinase has also been implicated in S phase checkpoint signaling downstream of the ATR and Chk1 kinases. Inactivation of Cdc7 in yeast results in arrest of cell growth with 1C DNA content after completion of the ongoing DNA replication. In contrast, conditional inactivation of Cdc7 in undifferentiated mouse embryonic stem (ES) cells leads to growth arrest with rapid cessation of DNA synthesis, suggesting requirement of Cdc7 functions for continuation of ongoing DNA synthesis. Furthermore, loss of Cdc7 function induces recombinational repair (nuclear Rad51 foci) and G2/M checkpoint responses (inhibition of Cdc2 kinase). Eventually, p53 becomes highly activated and the cells undergo massive p53-dependent apoptosis. Thus, defective origin activation in mammalian cells can generate DNA replication checkpoint signals. ...

2003-11-27

83

Morphologie des cellules de levure et la reproduction sexuelle - Apercu general et quelques considerations

Numerous studies have investigated degradation of individual endocrine disrupting compounds (EDCs) in lab or natural waters. However, natural variations in water matrices and mixtures of EDCs in the environment may confound analysis of the treatment efficiency. Because chemical based analytical methods cannot represent the combined or synergistic activities between water quality parameters and/or the EDC mixtures at environmentally relevant concentrations ({mu}g L{sup -1}-ng L{sup -1}), bioanalytical assessments of residual estrogenic activity in treated water were used to evaluate the performance of the UV based advanced oxidation process for estrogenic contaminants in water. Four EDCs including estradiol (E{sub 2}), ethinyl estradiol (EE{sub 2}), bisphenol-A (BPA) and nonylphenol (NP) were spiked individually or as a mixture at {mu}g L{sup -1}-ng L{sup -1} in laboratory or natural river water. The removal rates of estrogenic activity were quantitatively evaluated ...

2007-04-15

84

British Library Electronic Table of Contents (United Kingdom)

Over the decades, basic research in life sciences has profited greatly from the study of the small unicellular fungal species Saccharomyces cerevisiae. This yeast turned out to be key for the identification and understanding of molecular mechanisms that underlay the basic functions of all eukaryotic cells. These include, but are not limited to, the regulatory mechanisms behind cellular reproduction (cell cycle control), cellular morphogenesis (cell polarity, cytoskeleton and membrane trafficking) and the management of cellular information (chromosome biology, transcription and translation). Rapid access to genomic information of many yeast species, combined with bioinformatics analyses, provide information on the evolutionary history of yeasts and the molecular ancestry of their constituen...

2011-01-01

85

Alcohol fermentation in olive oil extraction effluents

Regulation and secretion of Xanthomonas virulence factors

Eight culture-collection yeast strains of various species and five newly isolated stains were tested for both growth in olive oil extraction effluents and fermentation of the sugars in the same media. The culture-collection yeast strains did not grow in an effluent containing 2.86% sugar (w/v), 8 g/litre phenolic substances, 4.58 g/litre titratable acidity and pH 4.96, whereas the newly isolated strains of Torulopsis sp. MK-1, Saccharomyces norbensis MC-1, S. oleaceus MC-2 and S. oleaginosus grew well and fermented the sugars. In the medium mentioned above, they produced alcohol in amounts of 1.63 to 1.38%, respectively. None of the yeasts grew in an olive oil extraction effluent vacuum-concentrated to over 13-14% of dry matter. The strain of T. sp. MK-1 showed a higher stability.

1989-01-01

86

British Library Electronic Table of Contents (United Kingdom)

Abstract Plant pathogenic bacteria of the genus Xanthomonas cause a variety of diseases in economically important monocotyledonous and dicotyledonous crop plants worldwide. Successful infection and bacterial multiplication in the host tissue often depend on the virulence factors secreted including adhesins, polysaccharides, LPS and degradative enzymes. One of the key pathogenicity factors is the type III secretion system, which injects effector proteins into the host cell cytosol to manipulate plant cellular processes such as basal defense to the benefit of the pathogen. The coordinated expression of bacterial virulence factors is orchestrated by quorum-sensing pathways, multiple two-component systems and transcriptional regulators such as Clp, Zur, FhrR, HrpX and HpaR. Furthermore, virule...

2010-01-01

87

Growth factor signalling in the regulation of -cell fate

British Library Electronic Table of Contents (United Kingdom)

Glucagon plays critical roles in regulating glucose homeostasis, mainly by counteracting the effects of insulin. Consequently, the dysregulated glucagon secretion that is evident in type 2 diabetes has significant implications in the pathophysiology of the disease. Glucagon secretion from pancreatic -cells has been suggested to be modulated by blood glucose, signals from the nervous system and endocrine components. In addition to these regulators, intraislet factors acting in a paracrine manner from neighbouring -cells are emerging as central modulator(s) of -cell biology. One of the most important of these paracrine factors, insulin, modulates glucagon secretion. Indeed, the -cell-specific insulin receptor knockout (IRKO) mouse manifests hypersecretion of glucagon in the postprandial stag...

2011-01-01

88

Variants within the yeast Ty sequence family encode a class of structurally conserved proteins.

The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription

The Ty transposable elements of Saccharomyces cerevisiae form a heterogeneous family within which two broad structural classes (I and II) exist. The two classes differ by two large substitutions and...Full Text Available

1985-06-11

89

The recruitment of acetylated and unacetylated tropomyosin to distinct actin polymers permits the discrete regulation of specific myosins in fission yeast

The mini-chromosome maintenance proteins Mcm2–7 are essential for DNA replication. They are loaded onto replication origins during G1 phase of the cell cycle to form a pre-replication complex...Full Text Available

2005-01-01

90

The fission yeast gene pmt1+ encodes a DNA methyltransferase homologue.

Tropomyosin (Tm) is a conserved dimeric coiled-coil protein, which forms polymers that curl around actin filaments in order to regulate actomyosin function. Acetylation of the Tm N-terminal...Full Text Available

2010-10-01

91

Structure-Function Study of the N-terminal Domain of Exocyst Subunit Sec3*

DNA methylation of cytosine residues is a widespread phenomenon and has been implicated in a number of biological processes in both prokaryotes and eukaryotes. This methylation occurs at the 5-position...Full Text Available

1995-01-25

92

Some highlights of research on aging with invertebrates, 2010

The exocyst is an evolutionarily conserved octameric complex involved in polarized exocytosis from yeast to humans. The Sec3 subunit of the exocyst acts as a spatial landmark for exocytosis through...Full Text Available

2010-04-02

93

SIRT1 contributes to telomere maintenance and augments global homologous recombination

This annual review focuses on invertebrate model organisms, which continue to yield fundamental new insights into mechanisms of aging. This year, the budding yeast has been used to understand how asymmetrical...Full Text Available

2011-02-01

94

PtdIns 3-Kinase Orchestrates Autophagosome Formation in Yeast

Yeast Sir2 deacetylase is a component of the silent information regulator (SIR) complex encompassing Sir2/Sir3/Sir4. Sir2 is recruited to telomeres through Rap1, and this complex spreads into subtelomeric...Full Text Available

2010-12-27

95

O2-dependent methionine auxotrophy in Cu,Zn superoxide dismutase-deficient mutants of Saccharomyces cerevisiae.

Eukaryotic cells can massively transport their own cytoplasmic contents into a lytic compartment, the vacuole/lysosome, for recycling through a conserved system called autophagy. The key process in...Full Text Available

2011-01-01

96

Nuclear Pore Complex Number and Distribution throughout the Saccharomyces cerevisiae Cell Cycle by Three-Dimensional Reconstruction from Electron Micrographs of Nuclear Envelopes

Mutant strains of the yeast Saccharomyces cerevisiae which lack functional Cu,Zn superoxide dismutase (SOD-1) do not grow aerobically unless supplemented with methionine. The molecular basis of this...Full Text Available

1990-04-01

97

Mitochondrial transmission during mating in Saccharomyces cerevisiae is determined by mitochondrial fusion and fission and the intramitochondrial segregation of mitochondrial DNA.

The number of nuclear pore complexes (NPCs) in individual nuclei of the yeast Saccharomyces cerevisiae was determined by computer-aided reconstruction of entire nuclei from electron...Full Text Available

1997-11-01

98

Mammalian Sirtuins and Energy Metabolism

To gain insight into the process of mitochondrial transmission in yeast, we directly labeled mitochondrial proteins and mitochondrial DNA (mtDNA) and observed their fate after the fusion of two cells....Full Text Available

1997-07-01

99

MAPPING THE INITIATOR BINDING TAF2 SUBUNIT IN THE STRUCTURE OF HYDRATED YEAST TFIID

Sirtuins are highly conserved NAD⁺-dependent protein deacetylases and/or ADP-ribosyltransferases that can extend the lifespan of several lower model organisms including yeast, worms and flies....Full Text Available

100

Legionella fairfieldensis sp. nov. isolated from cooling tower waters in Australia.

SummaryThe general transcription factor TFIID is a large multi-subunit complex required for the transcription of most protein-encoding genes by RNA polymerase II. Taking advantage...Full Text Available

2009-03-11

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101

Insights into Cdc13 dependent telomere length regulation

Three Legionella-like organisms were isolated from water from the cooling towers of two Australian institutions. The strains grew on buffered charcoal-yeast extract (BCYE) agar but not on BCYE agar...Full Text Available

1991-03-01

102

Increased Ethanol Productivity in Xylose-Utilizing Saccharomyces cerevisiae via a Randomly Mutagenized Xylose Reductase?

Cdc13 is a single stranded telomere binding protein that specifically localizes to the telomere ends of budding yeasts and is essential for cell viability. It caps the ends of chromosomes thus preventing...Full Text Available

103

Improving yield of industrial biomass propagation by increasing the Trx2p dosage

Baker's yeast (Saccharomyces cerevisiae) has been genetically engineered to ferment the pentose sugar xylose present in lignocellulose biomass. One of the reactions controlling the...Full Text Available

2010-12-01

104

Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels

The beneficial effect of improving yeast redox response by increasing thioredoxin levels has been shown. Decreased lipid and protein oxidation is reflected in an increased biomass yield. In addition,...Full Text Available

2010-09-01

105

Identification and Characterization of CPS1 as a Hyaluronic Acid Synthase Contributing to the Pathogenesis of Cryptococcus neoformans Infection?

Calorie restriction (CR) induces a metabolic shift towards mitochondrial respiration; however, molecular mechanisms underlying CR remain unclear. Recent studies suggest that CR-induced mitochondrial...Full Text Available

106

Fuzzy-decision-making problems of fuel ethanol production using a genetically engineered yeast

Cryptococcus neoformans is a pathogenic yeast that often causes devastating meningoencephalitis in immunocompromised individuals. We have previously identified the C. neoformans...Full Text Available

2007-08-01

107

Fermentability of Corn Syrups with Different Dextrose Equivalents Added to Various Grape Juices¹

A fuzzy-decision-making procedure is applied to find the optimal feed policy of a fed-batch fermentation process for fuel ethanol production using a genetically engineered Saccharomyces yeast 1400 (pLNH33). The policy consisted of feed flow rate, feed concentration, and fermentation time. The recombinant yeast 1400 (pLNH33) can utilize glucose and xylose simultaneously to produce ethanol. However, the parent yeast utilizes glucose only. A partially selective model is used to describe the kinetic behavior of the process. In this study, this partially selective fermentation process is formulated as a general multiple-objective optimal control problem. By using an assigned membership function for each of the objectives, the general multiple-objective optimization problem can be converted into a maximizing decision problem. In order to obtain a global solution, a hybrid method of differential evolution is introduced to solve ...

1998-08-01

108

Expansions, contractions, and fragility of the spinocerebellar ataxia type 10 pentanucleotide repeat in yeast

It was found that neither the enzymes of the grapes nor those of wine yeast Saccharomyces ellipsoideus strain 223 attacked the higher polysaccharides present in corn syrups. The alcohol...Full Text Available

1967-03-01

109

Exit from the Golgi Is Required for the Expansion of the Autophagosomal Phagophore in Yeast Saccharomyces cerevisiae

Spinocerebellar ataxia 10 (SCA10) is an autosomal dominant disease caused by large-scale expansions of the (ATTCT)_n repeat within an intron of the human ATXN10 gene. In contrast...Full Text Available

2011-02-15

110

Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules

The delivery of proteins and organelles to the vacuole by autophagy involves membrane rearrangements that result in the formation of large vesicles called autophagosomes. The mechanism underlying autophagosome...Full Text Available

2010-07-01

111

Cloning and Analysis of a Candida albicans Gene That Affects Cell Surface Hydrophobicity

The programming of cellular networks to achieve new biological functions depends on the development of genetic tools that link the presence of a molecular signal to gene-regulatory activity. Recently,...Full Text Available

2011-07-01

112

Chemical complementation: A reaction-independent genetic assay for enzyme catalysis

The opportunistic pathogenic yeast Candida albicans exhibits growth phase-dependent changes in cell surface hydrophobicity, which has been correlated with adhesion to host tissues....Full Text Available

2001-06-01

113

Biotransformation of Explosives by the Old Yellow Enzyme Family of Flavoproteins

A high-throughput assay for enzyme activity has been developed that is reaction independent. In this assay, a small-molecule yeast three-hybrid system is used to link enzyme catalysis to transcription...Full Text Available

2002-12-24

114

Arabidopsis thaliana Chromosome 4 Replicates in Two Phases That Correlate with Chromatin State

Several independent studies of bacterial degradation of nitrate ester explosives have demonstrated the involvement of flavin-dependent oxidoreductases related to the old yellow enzyme (OYE) of yeast....Full Text Available

2004-06-01

115

Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

DNA replication programs have been studied extensively in yeast and animal systems, where they have been shown to correlate with gene expression and certain epigenetic modifications. Despite the conservation...Full Text Available

2010-06-01

116

Affinity maturation of human botulinum neurotoxin antibodies by light chain shuffling via yeast mating

BackgroundThe production of high yields of recombinant proteins is an enduring bottleneck in the post-genomic sciences that has yet to be addressed in a truly rational manner. Typically...Full Text Available

117

A Novel Form of Transcriptional Silencing by Sum1-1 Requires Hst1 and the Origin Recognition Complex

Botulism is caused by the botulinum neurotoxins (BoNTs), the most poisonous substance known. Because of the high potency of BoNT, development of diagnostic and therapeutic antibodies for botulism requires...Full Text Available

2010-04-01

118

Yeast as a Model System to Study Tau Biology

In the yeast Saccharomyces cerevisiae, a and α mating-type information is stored in transcriptionally silenced cassettes called HML and HMR....Full Text Available

2001-05-01

119

Cell cultures are more sensitive than Saccharamoyces cervisiae tests for assessing the toxicity of aquatic pollutants

Hyperphosphorylated and aggregated human protein tau constitutes a hallmark of a multitude of neurodegenerative diseases called tauopathies, exemplified by Alzheimer's disease. In spite of an enormous amount of research performed on tau biology, several crucial questions concerning the mechanisms of tau toxicity remain unanswered. In this paper we will highlight some of the processes involved in tau biology and pathology, focusing on tau phosphorylation and the interplay with oxidative stress. In addition, we will introduce the development of a human tau-expressing yeast model, and discuss some crucial results obtained in this model, highlighting its potential in the elucidation of cellular processes leading to tau toxicity.

2011-04-06

120

The effect of substrate modification on microbial growth on surfaces

Cultured fish and human cells have been used as bioassay systems for the evaluation of the toxicity of aquatic pollutants. Numerous assays using bacteria and yeast have also been used for such purposes. The authors report the toxicity of aquatic pollutants (Cd, Hg, and Ni), using cell culture systems and the yeast Saccharomyces cervisiae test. Cd, Hg, and Ni were chosen as model compounds of pollutants because the related toxicity is now fairly well established.

1988-07-01

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121

Wnt3a Induces Myofibroblast Differentiation by Upregulating TGF-? Signaling Through SMAD2 in a ?-Catenin-Dependent Manner

The principle aim of the program was to produce a novel, non-leaching antimicrobial surface for commercial development and future use in the liquid food packaging industry. Antimicrobial surfaces which exist presently have been produced to combat the growth of prokaryotic organisms and usually function as slow release systems. A system which could inhibit eukaryotic growth without contaminating the surrounding 'environment' with the inhibitor was considered of great commercial importance. The remit of this study was concerned with creating a surface which could control the growth of eukaryotic organisms found in fruit juice with particular interest in the yeast, Saccharomyces cerevisiae. Putative antimicrobial surfaces were created by the chemical modification of the test substrate polymers; nylon and ethylvinyl alcohol (EVOH). Surfaces were chemically modified by the covalent coupling of antimicrobial agents known to be active against the ...

1998-07-01

122

The effects of nicotine, hexamethonium and ethanol on the secretion of the antidiuretic and oxytocic hormones of the rat

Growing evidence suggests the Wnt family of secreted glycoproteins and their associated signaling pathways, linked to development, are recapitulated during wound repair and regeneration events. However,...Full Text Available

123

The effect of IL6-174C/G polymorphism on postprandial triglyceride metabolism in the GOLDN study*

The actions of nicotine, hexamethonium, and ethanol on the hypothalamo-hypophysial system have been investigated in the rat. The antidiuretic action of nicotine was not inhibited by ethanol, nor by...Full Text Available

1957-12-01

124

Some characteristics of a secreted chlamydial antigen recognized by IgG from C. trachomatis patient sera.

Chronically elevated interleukin-6 (IL-6) affects lipid and lipoprotein metabolism. Individuals genetically predisposed to higher IL-6 secretion may be at risk of dyslipidemia, especially during the...Full Text Available

2008-08-01

125

Secretion of alpha-hemolysin by bovine mammary isolates of Staphylococcus aureus.

Chlamydia trachomatis serovars release a glycolipid antigen (GLXA) into the culture supernatant during the infective cycle. This antigen is recognized by IgG isolated from humans with a natural chlamydial...Full Text Available

1989-12-01

126

Role of Bv8 in neutrophil-dependent angiogenesis in a transgenic model of cancer progression

A total of 262 strains of Staphylococcus aureus isolated from the mammary gland of dairy cows were examined for the production of alpha-hemolysin. Strains were cultured in a liquid medium of casein...Full Text Available

1992-07-01

127

Quantum secure direct communication scheme using a W state and teleportation

The secreted Bv8 protein has been recently characterized as a regulator of myeloid cell mobilization and a neutrophil-derived mediator of tumor angiogenesis in several xenografts, but its role in tumor...Full Text Available

2008-02-19

128

Peptides of human bronchial mucus glycoproteins. Size determination by electron microscopy and by biosynthetic experiments.

A theoretical scheme for quantum secure direct communication (QSDC) is proposed, where a three-qubit symmetric W state functions as a quantum channel. Two legitimate communicators can transmit their secret information by using quantum teleportation and local measurements.

2006-11-01

129

Parallel Evolution of a Type IV Secretion System in Radiating Lineages of the Host-Restricted Bacterial Pathogen Bartonella

Secreted human bronchial mucins, directly collected from macroscopically healthy bronchial mucosa, were prepared in the presence of six proteinase inhibitors, and analysed by electron microscopy. These...Full Text Available

1987-11-15

130

Molecular cloning and nucleotide sequence of the beta-lytic protease gene from Achromobacter lyticus.

Adaptive radiation is the rapid origination of multiple species from a single ancestor as the result of concurrent adaptation to disparate environments. This fundamental evolutionary process is considered...Full Text Available

2011-02-01

131

Introduction of the CIITA gene into tumor cells produces exosomes with enhanced anti-tumor effects

Two bacteriolytic enzymes secreted by Achromobacter lyticus M497-1 were purified and identified as being very similar (considering their amino acid composition and N-terminal sequence) to alpha- and...Full Text Available

1990-11-01

132

Interactive Cytokine Regulation of Synoviocyte Lubricant Secretion

Exosomes are small membrane vesicles secreted from various types of cells. Tumor-derived exosomes contain MHC class I molecules and tumor-specific antigens, receiving attention as a potential cancer...Full Text Available

2011-05-31

133

Insulin and Glucagon Regulate Pancreatic ?-Cell Proliferation

Cytokine regulation of synovial fluid (SF) lubricants, hyaluronan (HA), and proteoglycan 4 (PRG4) is important in health, injury, and disease of synovial joints, and may also provide powerful regulation...Full Text Available

2010-04-01

134

In vitro antigen-induced antibody responses to hepatitis B surface antigen in man. Kinetic and cellular requirements.

Type 2 diabetes mellitus (T2DM) results from insulin resistance and β-cell dysfunction, in the setting of hyperglucagonemia. Glucagon is a 29 amino acid peptide hormone, which is secreted from...Full Text Available

135

Importance of gastrin in the pathogenesis and treatment of gastric tumors

In this report we define the parameters of the human immune response after immunization with hepatitis B vaccine. 2 wk after booster immunization, there is significant spontaneous secretion of antibody...Full Text Available

1984-10-01

136

Glucose Polyester Biosynthesis. Purification and Characterization of a Glucose Acyltransferase1

In addition to regulating acid secretion, the gastric antral hormone gastrin regulates several important cellular processes in the gastric epithelium including proliferation, apoptosis, migration, invasion,...Full Text Available

2009-01-07

137

Excretion of para-aminohippurate in the isolated perfused rat kidney: net secretion and net reabsorption.

Glandular trichomes of the wild tomato species Lycopersicon pennellii secrete 2,3,4-O-tri-acyl-glucose (-Glc), which contributes to insect resistance. A Glc acyltransferase...Full Text Available

1999-10-01

138

Effect of a maltodextrin-electrolyte, a maltodextrin-nutrient-electrolyte and a standard electrolyte solution on water and electrolyte fluxes in the secreting rat intestine.

1. The excretion of para-aminohippurate (PAH) in the isolated perfused rat kidney was examined over a wide range of perfusate PAH concentrations (15 microM to 6 mM). PAH excretion increased steadily...Full Text Available

1988-03-01

139

Developmental Changes in Pituitary Adenylate Cyclase Activating Polypeptide Expression during the Perinatal Period: Possible Role in Fetal Gonadotroph Regulation

The effects of a maltodextrin (dextrose equivalent 12)-electrolyte solution and a maltodextrin-electrolyte solution with added nutrients on net water and electrolyte transport in the secreting rat intestine was compared with the citrate-World Health Organization oral rehydration solution to determine the need for a clinical trial to evaluate the efficacy of these maltodextrin solutions in acute diarrhoea treatment. Cholera toxin consistently produced net water secretion (-36.5 +/- 9.9 mean +/- SEM microliter/min/g dry weight of intestine). All three solutions reversed the cholera toxin-induced net intestinal water secretion to net absorption. Significantly greater net water absorption occurred from the maltodextrin-electrolyte solution compared to the World Health Organization solution (P < 0.05) but not when compared to the maltodextrin-electrolyte-nutrient solution. Net sodium, potassium and chloride fluxes due to the ...

1993-12-01

140

Der beschleunigte Fortschritt

Normal reproductive functioning may require secretion of LH independently of FSH. Variation in GnRH pulse frequency and inhibin negative feedback are mechanisms for differential gonadotropin regulation;...Full Text Available

2009-10-01

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141

CERN Multimedia

Since 50 years, the scientists in European Center for Nuclear Research in Geneva, are looking for the secrets of the nature. Their standard Model would explains a lot of things; but the single question "What is the mass?" still does not have an answer

2004-01-01

142

Construction of a novel bifunctional biogenic amine receptor by two point mutations of the H2-histamine receptor.

Computational simulation of vasopressin secretion using a rat model of the water and electrolyte homeostasis

BACKGROUND: H2-histamine receptors mediate a wide range of physiological functions extending from stimulation of gastric acid secretion to induction of human promyelocyte differentiation. We have previously...Full Text Available

1995-03-01

143

Clouded leopards, the secretive top-carnivore of South-East Asian rainforests: their distribution, status and conservation needs in Sabah, Malaysia

BackgroundIn mammals, vasopressin (AVP) is released from magnocellular neurons of the hypothalamus when osmotic pressure exceeds a fixed set-point. AVP participates to the hydromineral...Full Text Available

144

Cathepsin B facilitates Autophagy mediated apoptosis in SPARC Overexpressed Primitive Neuroectodermal Tumor cells

BackgroundThe continued depletion of tropical rainforests and fragmentation of natural habitats has led to significant ecological changes which place most top carnivores under heavy...Full Text Available

145

Capsaicin-sensitive sensory neurons are involved in the plasma catecholamine response of rats to selective stressors.

Medulloblastoma and neuroblastoma belong to a group of neoplasms designated as primitive neuroectodermal tumors (PNETs). Secreted Protein, Acidic and Rich in Cysteine (SPARC) is a matrix-associated...Full Text Available

2010-10-01

146

Atrial natriuretic peptide signal pathway upregulated in stomach of streptozotocin-induced diabetic mice

1. The effect of capsaicin pre-treatment on adrenal catecholamine (CA) secretion in response to stress is controversial. In earlier experiments performed under pentobarbitone anaesthesia, the release...Full Text Available

1991-02-01

147

Association between a functional polymorphism in the serotonin transporter gene and diarrhoea predominant irritable bowel syndrome in women

AIM: To investigate atrial natriuretic peptide (ANP) secretion from gastric mucosa and the relationship between the ANP/natriuretic peptide receptor type A (NPR-A) pathway and diabetic gastroparesis.METHODS:...Full Text Available

2010-01-07

148

An Integrated Genomics Approach to Define Niche Establishment by Rhodococcus fascians1[C][W][OA

Background and aims: Serotonin (5-hydroxtryptamine, 5-HT) is an important factor in gut function, playing key roles in intestinal peristalsis and secretion, and in sensory...Full Text Available

2004-10-01

149

Adhesion of DOPA-Functionalized Model Membranes to Hard and Soft Surfaces

Rhodococcus fascians is a Gram-positive phytopathogen that induces shooty hyperplasia on its hosts through the secretion of cytokinins. Global transcriptomics using microarrays combined...Full Text Available

2009-03-01

150

Acetic Acid Bacterial Biota of the Pink Sugar Cane Mealybug, Saccharococcus sacchari, and Its Environs

The adhesive proteins secreted by marine mussels form a natural glue that cures rapidly to form strong and durable bonds in aqueous environments. These mussel adhesive proteins contain an unusual...Full Text Available

2009-01-01

151

AGEs Secreted by Bacteria Are Involved in the Inflammatory Response

Saccharococcus sacchari is the primary colonizer of the developing “sterile” tissue between the leaf sheath and stem of sugar cane. The honeydew secreted by the mealybugs...Full Text Available

1990-03-01

152

A mathematical/physiological model of parathyroid hormone secretion in response to blood-ionized calcium lowering in vivo.

Advanced Glycated End Products (AGEs) are formed by non-enzymatic protein glycation and are implicated in several physiological aspects including cell aging and diseases. Recent data indicate that bacteria...Full Text Available

153

DEFF Research Database (Denmark)

The aim of the present study was to test a mathematical model of the biochemical processes in the parathyroid glands responsible for the secretion of parathyroid hormone resulting from extracellular calcium reduction. A double exponential curve described the parathyroid hormone secretion induced by rapid lowering of blood-ionized calcium in humans with normal as well as abnormal parathyroid tissue. Our data show that it was possible to establish a simple mathematical model of the parathyroid hormone response to blood-ionized calcium lowering, sufficient to fit experimental data obtained from patients with abnormal and normal parathyroid tissue. The fitted parameters showed no significant differences between patients with insulin-dependent diabetes mellitus and controls. In primary hyperparathyroidism, the parathyroid hormone production and steady-state transport across the cell membrane were increased, probably due to the larger amount of ...

1997-01-01

154

A genomic and proteomic investigation of the impact of preimplantation factor on human decidual cells

Quantum Secure Direct Communication with Four-Particle Genuine Entangled State and Dense Coding

OBJECTIVEPreimplantation factor (PIF) is a novel, 15 amino acid peptide, secreted by viable embryos. This study aims to elucidate PIF’s effects in human endometrial...Full Text Available

2010-05-01

155

Yeast artificial chromosome libraries containing large inserts from mouse and human DNA

A quantum secure direct communication scheme using dense coding is proposed. At first, the sender (Alice) prepares four-particle genuine entangled states and shares them with the receiver (Bob) by sending two particles in each entangled state to him. Secondly, Alice encodes secret information by performing the unitary transformations on her particles and transmits them to Bob. Finally, Bob performs the joint measurements on his particles to decode the secret information. The two-step security test guarantees the security of communication.

2009-07-01

156

Homology analyses of the protein sequences of fatty acid synthases from chicken liver, rat mammary gland, and yeast

Yeast artificial chromosome (YAC) libraries have been difficult to construct with average insert sizes >400 kilobase pairs when DNA is size-fractionated in low-melting-point agarose. By using yeast chromosomes in mock cloning experiments, the authors found that polyamines should be present whenever agarose containing high molecular weight DNA is melted to protect DNA from degradation. By incorporating polyamines during the cloning procedure, they constructed YAC libraries from mouse and human DNA with average insert sizes of 700 and 620 kilobase pairs, respectively. Several genome equivalents of these YAC libraries were replicated onto the surface of many duplicate agar plates using a 40,000 multipin transfer device. High-density filter replicas were screened by hybridization, and 70 mouse YAC clones from 31 loci and 132 human YAC clones from 49 loci were isolated.

1991-05-15

157

Assessment of the role of oxygen and mitochondria in heat shock induction of radiation and thermal resistance in Saccharomyces cerevisiae

Homology analyses of the protein sequences of chicken liver and rat mammary gland fatty acid synthases were carried out. The amino acid sequences of the chicken and rat enzymes are 67% identical. If conservative substitutions are allowed, 78% of the amino acids are matched. A region of low homologies exists between the functional domains, in particular around amino acid residues 1059-1264 of the chicken enzyme. Homologies between the active sites of chicken and rat and of chicken and yeast enzymes have been analyzed by an alignment method. A high degree of homology exists between the active sites of the chicken and rat enzymes. However, the chicken and yeast enzymes show a lower degree of homology. The DADPH-binding dinucleotide folds of the {beta}-ketoacyl reductase and the enoyl reductase sites were identified by comparison with a known consensus sequence for the DADP- and FAD-binding dinucleotide folds. The active sites of all of the enzymes ...

1989-11-01

158

Removal of uranium from solution using residual brewery yeast: combined biosorption and precipitation

In response to a heat shock, the yeast Saccharomyces cerevisiae undergoes a large increase in its resistance to heat and, by the induction of its recombinational DNA repair capacity, a corresponding increase in resistance to radiation. Yeast which lack mitochondrial DNA, mitochondria-controlled protein synthetic apparatus, aerobic respiration, and electron transport (rho/sup 0/ strain) were used to assess the role of O/sub 2/, mitochondria, and oxidative processes controlled by mitochondria in the induction of these resistances. We have found that rho/sup 0/ yeast grown and heat shocked in either the presence or absence of O/sub 2/ are capable of developing both radiation and heat resistance. We conclude that neither the stress signal nor its cellular consequences of induced heat and radiation resistance are directly dependent on O/sub 2/, mitochondrial DNA, or mitochondria-controlled protein synthetic or oxidative ...

1983-10-01

159

Pectinolytic yeast isolates for cold-active polygalacturonase production

Whilst unwashed preparations of biomass from a local brewery had an apparent maximum biosorption capacity for uranium of 360 mg/g (dry weight biomass) washing reduced this maximum to 150 mg/g. Homogenization of both biomass preparations and recovery of cellular debris had no significant effect on the maximum biosorption capacities although at lower equilibrium concentrations of uranium differences in the biosorption capacities were detected. When unwashed biomass was retained by a semi-permeable membrane 40% of uranium used in the experiments precipitated outside that membrane. Therefore a significant proportion of the uranium removed from solution, and previously attributed to biosorption by the yeast biomass, resulted from precipitation brought about by interaction with low molecular weight components loosely associated with the biomass. (Author).

1997-04-01

160

British Library Electronic Table of Contents (United Kingdom)

Pectin rich cold stored spoiled fruits, vegetables and cold soils were screened and different pectinolytic isolates were obtained by enrichment culturing and ruthenium red plate assay. Among the primary isolates 10-15% were yeast isolates. Six isolates with higher zones of pectin hydrolysis were selected and tested for polygalacturonase (PGU) production at room temperature (25 degrees C) and at 5 degrees C. One isolate identified as Saccharomyces sp. with highest polygalacturonase activity at 5 degrees C was used for enzyme production using raw fruit pectins as substrates. The isolate was identified by preliminary cultural, morphological and sugar fermentation tests. PGU production was high in raw pectin substrates like orange peel (21 U/ml), apple peel (20 U/ml ), mango peel (19 U/ml), ...

2011-01-01

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161

Microbiological quality and biophenol content of hot air-dried Thassos cv. table olives upon storage

British Library Electronic Table of Contents (United Kingdom)

Abstract Microbiological quality and biophenol content evolution was studied in minimally processed Thassos cv table olives by hot air dehydration under mild conditions (40C, 24-h, aw-=-0.893) and storage under characteristic packaging conditions (vacuum, 100% N2 and air) at 4 and 20C over a period of 180 days. No salt was used in the production line or packaging. The undesirable microorganisms (Enterobacteriaceae, Staphylococcus aureus, Bacillus, Clostridium) were undetectable until the end of the storage period. Also, modified atmospheres prevented fungal growth at both temperatures apart from the samples stored in air, in which Penicillium and Aspergillus spp. were identified. At 20C, a coexistence of mesophilic bacteria and yeasts occurred. At 4C, yeasts were the predominant microflora...

2011-01-01

162

Extrinsic allergic alveolitis induced by the yeast Debaryomyces hansenii.

Effects of dietary glucosylceramide on dermatitis in atopic dermatitis model mice

A 65-yr-old female developed cough, fever and dyspnoea following repeated exposure to a home ultrasonic humidifier. High-resolution computed tomography showed ground-glass opacity in both lung fields. Arterial blood gas analysis gave an oxygen tension of 8.38 kPa (63 Torr). Pulmonary function testing revealed restrictive ventilatory impairment with a reduction in the diffusing capacity. The diagnosis of extrinsic allergic alveolitis (EAA) was confirmed by radiographic findings, pathological evidence of alveolitis and reproductive development by a provocation test to the humidifier water. The yeast Debaryomyces Hansenii was the only microorganism cultured from the water of the humidifier. The double diffusion precipitating test and lymphocyte proliferative response was positive for an extract of D. Hansenii, providing evidence to incriminate this fungus. This is the first described case of EAA caused by D. Hansenii. PMID:12449192

2002-11-01

163

British Library Electronic Table of Contents (United Kingdom)

The effects of dietary plant and yeast cerebroside (glucosylceramide), a major sphingolipid in plants and yeast, on atopic dermatitis (AD) like symptoms were investigated in a mouse model. After 7 wk of feeding with a diet containing maize glucosylceramide, plasma IgE levels became significantly lower and in contrast, the levels of interleukin (IL)-12, which induces cellular immunity, became significantly higher in the AD mice than in the controls. However, the sphingolipid constituents of the skin fraction in the maize glucosylceramide fed group did not contain sphingoid bases of plant origin, such as 8-unsaturated sphingoid bases. The results of the present study indicated that dietary plant glucosylceramide prevented AD-like symptoms in AD model mice via regulation of Th1/Th2 balance. P...

2010-01-01

164

Mechanisms of renal handling of lead

Manganese Induces IGF-1 and Cyclooxygenase-2 Gene Expressions in the Basal Hypothalamus during Prepubertal Female Development

The relative roles of glomerular filtration, tubular reabsorption and tubular secretion in lead handling were evaluated using renal clearance technique, stop-flow analysis, and renal lead accumulation. In addition, the effect of altering acid-base status on renal handling of Pb was determined. Clearance of ultrafilterable lead was measured in anesthetized dogs during infusion of "2"0"3Pb with small doses of carrier Pb(0.5 to 50 #mu#g/kg/h). Filterable lead concentration was estimated by in vitro ultrafiltration of non-anticoagulated plasma. Fractional excretion of Pb was calculated using creatinine clearance to estimate glomerular filtration rate. Dogs in acute metabolic acidosis had the lowest fractional excretion of ultrafilterable Pb (< 0.1); those in alkalosis had high fractional Pb excretion (0.75 to 2.5) while normal dogs were intermediate (0.1 to .4). The fractional excretion of Pb during metabolic alkalosis varied with the dose of Pb administered; only ...

165

British Library Electronic Table of Contents (United Kingdom)

Precocious puberty is a significant child health problem, especially in girls, because 95% of cases are idiopathic. Our earlier studies demonstrated that low-dose levels of manganese (Mn) caused precocious puberty via stimulating the secretion of luteinizing hormone-releasing hormone (LHRH). Because glial-neuronal communications are important for the activation of LHRH secretion at puberty, we investigated the effects of prepubertal Mn exposure on specific glial-derived puberty-related genes known to affect neuronal LHRH release. Animals were supplemented with MnCl2 (10 mg/kg) or saline by gastric gavage from day 12 until day 22 or day 29, then decapitated, and brains removed. The site of LHRH release is the medial basal hypothalamus (MBH), and tissues from this area were analyzed by real-...

2011-01-01

166

Eating the enemy in Crohn's disease

British Library Electronic Table of Contents (United Kingdom)

Several old and new observations suggest the existence in Crohn's disease of a phagocytic disorder of macrophages related to impaired bactericidal activity of host cells or to the presence of invasive bacteria that have developed strategies to counteract macrophage killing. It was recently reported that disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease. Secretion of proinflammatory cytokines by CD macrophages was impaired in response to E. coli or specific Toll-like receptor agonists. In addition, major advances in the etiology of Crohn's disease came from the existence of polymorphism in NOD2 and autophagy-related susceptibility genes (ATG16L1 and IRGM) in patients and from the identification of the presence of adhere...

2010-01-01

167

Yeast ribosomal protein L1 is required for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits.

Wide cross-species aminoacyl-tRNA synthetase replacement in vivo: yeast cytoplasmic alanine enzyme replaced by human polymyositis serum antigen.

Ribosomal protein L1 from Saccharomyces cerevisiae binds 5S rRNA and can be released from intact 60S ribosomal subunits as an L1-5S ribonucleoprotein (RNP) particle. To understand the nature of the...Full Text Available

1993-05-01

168

The k43 gene, required for chorion gene amplification and diploid cell chromosome replication, encodes the Drosophila homolog of yeast origin recognition complex subunit 2

Because of variations in tRNA sequences in evolution, tRNA synthetases either do not acylate their cognate tRNAs from other organisms or execute misacylations which can be deleterious in vivo. We report...Full Text Available

1995-05-23

169

The distribution of active RNA polymerase II along the transcribed region is gene-specific and controlled by elongation factors

Lethal alleles of the Drosophila k43 gene result in small or missing imaginal discs, greatly reduced mitotic index, and fragmented and abnormally condensed chromosomes. A female-sterile...Full Text Available

1997-04-15

170

Specific requirement of the chromatin modifier mSin3B in cell cycle exit and cellular differentiation

In order to study the intragenic profiles of active transcription, we determined the relative levels of active RNA polymerase II present at the 3′- and 5′-ends of 261 yeast genes by...Full Text Available

2010-08-01

171

SWI/SNF and Asf1 Independently Promote Derepression of the DNA Damage Response Genes under Conditions of Replication Stress

The Sin3-histone deacetylase (HDAC) corepressor complex is conserved from yeast to humans. Mammals possess two highly related Sin3 proteins, mSin3A and mSin3B, which serve as scaffolds tethering HDAC...Full Text Available

2008-03-18

172

Primary organization of nucleosomal core particles is invariable in repressed and active nuclei from animal, plant and yeast cells.

The histone chaperone Asf1 and the chromatin remodeler SWI/SNF have been separately implicated in derepression of the DNA damage response (DDR) genes in yeast cells treated with genotoxins that cause...Full Text Available

173

Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli

A refined map for the linear arrangement of histones along DNA in nucleosomal core particles has been determined by DNA-protein crosslinking. On one strand of 145-bp core DNA, histones are aligned in...Full Text Available

1985-05-24

174

BackgroundThe large sensitivity, high reproducibility and essentially unlimited dynamic range of real-time PCR to measure gene expression in complex samples provides the opportunity...Full Text Available

175

Molecular methods

Metabolomic analysis of the plant pathogenic fungi Fusarium graminearum and Fusarium culmorum

Chapter 5, describes some of the most important molecular methods used in the study of chromosome structure and function. The methods discussed include fragmentation of DNA, cloning, flow cytometry and chromosome sorting, is situ hybridization, polymerase chain reaction (PCR), and yeast artificial chromosomes (YACs). 18 refs., 3 figs., 1 tab.

1993-12-31

176

Environmental Research Database

DescriptionThis project is part of the BBSRCs special initiative on plant and microbial metabolomics. The project will primarily focus on the trichothecene mycotoxin producing Ascomycete fungus Fusarium graminearum (Fg) which causes ear blight disease of small grain cereals. The project aims to explore the metabolome of various wild-type and single gene deletion Fg strains and to compare some of these with the identical gene mutation in the budding yeast, S. cerevisiae (Sc) and the saprophytic filamentous [continued...

2008-01-31

177

Genome-wide analysis of N¹-methyl-adenosine modification in human tRNAs

Functional and Topological Analysis of Yeast Acyl-CoA:Diacylglycerol Acyltransferase 2, an Endoplasmic Reticulum Enzyme Essential for Triacylglycerol Biosynthesis*

The N¹-methyl-Adenosine (m¹A58) modification at the conserved nucleotide 58 in the TΨC loop is present in most eukaryotic tRNAs. In yeast, m¹A58 modification...Full Text Available

2010-07-01

178

Biological solubilization of coal in aqueous and nonaqueous media

Acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20) is a membrane protein present mainly in the endoplasmic reticulum. It catalyzes the final and committed step in the biosynthesis of triacylglycerol,...Full Text Available

2011-04-15

179

Assembly of 60S ribosomal subunits is perturbed in temperature-sensitive yeast mutants defective in ribosomal protein L16.

Current investigations into the biological solubilization of coal with microorganisms focus on the production of solubilizing activity in fungi. Test organisms for this work include a species of the yeast Candida previously isolated from a lignite outcrop, and P. chrysosporium, a filamentous higher fungus which has played a major role in lignin biodegradation research. The studies described are primarily exploratory in nature, and are fundamental to the design of more sophisticated inquiries into the physiology of fungal coal solubilization.

1987-01-01

180

[Hormonal disorders in chronic alcoholic intoxication].

Temperature-sensitive mutants defective in 60S ribosomal subunit protein L16 of Saccharomyces cerevisiae were isolated through hydroxylamine mutagenesis of the RPL16B gene and plasmid shuffling. Two...Full Text Available

1991-11-01

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181

Three-Party Simultaneous Quantum Secure Direct Communication Scheme with EPR Pairs

Hormone levels were examined in the venous blood in 54 men suffering from stage 2 alcoholism and in 30 normal subjects, using a radioimmunochemical assay. The alcoholics were found to have a statistically significant increase in prolactin and a decrease in testosterone. A definite ratio in the secretion of these hormones differing from that in the control group was elucidated. This ratio (prolactin-testosterone index) is proposed as a diagnostic test of alcoholism. PMID:6441404

1984-01-01

182

The normal developmental regulation of a cloned sgs3 'glue' gene chromosomally integrated in Drosophila melanogaster by P element transformation.

We present a scheme for three-party simultaneous quantum secure direct communication by using EPR pairs. In the scheme, three legitimate parties can simultaneously exchange their secret messages. It is also proved to be secure against the intercept-and-resend attack, the disturbance attack and the entangled-and-measure attack.

2007-09-01

183

Staphylococcus aureus Enterotoxin D Is Secreted in Milk and Stimulates Specific Antibody Responses in Cows in the Course of Experimental Intramammary Infection

A 7-kb genomic segment containing the coding sequence for the Drosophila melanogaster Formosa variant of salivary gland secretion protein 3 (sgs3) has been inserted into the snw y, bw, st strain of...Full Text Available

1983-01-01

184

Specific Sequence Motif of 8-Cys Repeats of TGF-? Binding Proteins, LTBPs, Creates a Hydrophobic Interaction Surface for Binding of Small Latent TGF-?

An enterotoxin D (SED)-producing strain of Staphylococcus aureus was used to infect one mammary gland of each of 17 lactating dairy cows. All glands became infected and shed bacteria...Full Text Available

2006-06-01

185

Signal transduction pathway controlling synthesis of a class of degradative enzymes in Bacillus subtilis: expression of the regulatory genes and analysis of mutations in degS and degU.

Transforming growth factor (TGF)-βs are secreted in large latent complexes consisting of TGF-β, its N-terminal latency-associated peptide (LAP) propeptide, and latent TGF-β...Full Text Available

2000-08-01

186

Seeking nature of God in Big Bang T1 the secrets to the beginning of the universe could lie underground in Switzerland.

The rates of synthesis of a class of both secreted and intracellular degradative enzymes in Bacillus subtilis are controlled by a signal transduction pathway defined by at least four regulatory genes:...Full Text Available

1990-02-01

187

CERN Multimedia

Joanna Geary joined a Birmingham University team of scientists working on the project in Geneva. Simond Hadley took the pictures. "The most exciting thing of all" says professor Peter Watkins with a smile, "is we have absolutely no idea what will happen until we switch it on."

2008-01-01

188

Public agency partnership: Hanford`s history artifacts as a communications tool

Proton-Transfer-Reaction Mass Spectrometry as a New Tool for Real Time Analysis of Root-Secreted Volatile Organic Compounds in Arabidopsis1

The Hanford Site in eastern Washington state currently is engaged in the largest waste cleanup in world history. In order to make informed decisions about remediation options, the public throughout the Pacific Northwest and the nation needs to understand the wastes that are present, their sources of generation, their composition, and their behavior in the environment. The fact that Hanford operations` were conducted in secret for over four decades presents a unique public information challenge to those who currently are responsible for communicating with the public.

1994-02-01

189

NHE8 is an intracellular cation/H⁺ exchanger in renal tubules of the yellow fever mosquito Aedes aegypti

Plant roots release about 5% to 20% of all photosynthetically-fixed carbon, and as a result create a carbon-rich environment for numerous rhizosphere organisms, including plant pathogens and symbiotic...Full Text Available

2004-05-01

190

Monocyte responses to sulfatide from Mycobacterium tuberculosis: inhibition of priming for enhanced release of superoxide, associated with increased secretion of interleukin-1 and tumor necrosis factor alpha, and altered protein phosphorylation.

The goal of this study was to identify and characterize the hypothesized apical cation/H⁺ exchanger responsible for K⁺ and/or Na⁺ secretion in...Full Text Available

2009-04-01

191

Influence of the high-affinity growth hormone (GH)-binding protein on plasma profiles of free and bound GH and on the apparent half-life of GH. Modeling analysis and clinical applications.

In monocytes, sulfatide, a lipid from Mycobacterium tuberculosis, blocked priming for enhanced release of superoxide (O2-) by the macrophage activating factors lipopolysaccharide, gamma interferon,...Full Text Available

1991-08-01

192

Hypothyroidism in Noninterferon Treated-HCV Infected Individuals Is Associated with Abnormalities in the Regulation of Th17 Cells

The discovery of a specific high-affinity growth hormone (GH) binding protein (GH-BP) in plasma adds complexity to the dynamics of GH secretion and clearance. Intuitive predictions are that such a protein...Full Text Available

1993-02-01

193

Growth differentiation factor-9 stimulates progesterone synthesis in granulosa cells via a prostaglandin E₂/EP2 receptor pathway

HCV-Ag-specific TH17 cells secrete IL17, a cytokine involved in autoimmune diseases and regulated by IL10 and TGF-b. 5–12% of patients with chronic HCV infection have hypothyroidism. We evaluated...Full Text Available

2010-01-01

194

Experimental realization of Dicke states of up to six qubits for multiparty quantum networking

Growth differentiation factor-9 (GDF-9), an oocyte-secreted member of the transforming growth factor β superfamily, progesterone receptor, cyclooxygenase 2 (Cox2; Ptgs2), and the EP2 prostaglandin...Full Text Available

2000-08-29

195

Diabetes-Induced Oxidative Stress Is Mediated by Ca²⁺-Independent Phospholipase A2 in Neutrophils

We report the first experimental generation and characterization of a six-photon Dicke state and demonstrate its remarkable versatility by projecting out four- and five-photon Dicke states, in addition to four-photon GHZ- and W-states. These multipartite states are studied by developing experimentally favorable characterization tools. Furthermore, we show that Dicke states have interesting applications in multiparty quantum networking protocols such as open-destination teleportation, telecloning and quantum secret sharing.

2009-01-01

196

Altered GABA_A receptor-mediated synaptic transmission disrupts the firing of gonadotropin-releasing hormone neurons in male mice under conditions that mimic steroid abuse

Neutrophils from people with poorly controlled diabetes present a primed phenotype and secrete excessive superoxide. Phospholipase A₂ (PLA₂)-derived arachidonic acid (AA)...Full Text Available

2010-02-01

197

ASPECTS OF PERFORMANCE EVALUATION OF WATERJET ...

Gonadotropin–releasing hormone (GnRH) neurons are the central regulators of reproduction. GABAergic transmission plays a critical role in pubertal activation of pulsatile GnRH secretion....Full Text Available

2010-05-12

198

Simulation of neutron detector efficiencies

... Propulsive efficiency is equivalent to the product of thrust efficiency and the hull/waterjet interaction efficiency. ... t'waterjet pump Ktorque repeated ...

1967-10-01

199

A - Z Index : Environmental Energy Technologies Division

No abstract prepared.

2002-03-01

200

Mutations in cyr1 and pat1 reveal pheromone-induced G1 arrest in the fission yeast Schizosaccharomyces pombe

Buildings Energy-Efficient Research Laboratories Energy Efficiency in Federal Facilities Energy Efficiency Standards Group Energy Efficient Windows Collaborative Energy &...

2011-07-15

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201

DEFF Research Database (Denmark)

Investigations into sexual differentiation and pheromone response in the fission yeast Schizosaccharomyces pombe are complicated by the need to first starve the cells of nitrogen. Most mating-related experiments are therefore performed on non-dividing cells. Here we overcome this problem by using two mutants that bypass the nutritional requirements and respond to the M-factor mating pheromone in rich medium. The first mutant lacks the cyr1 gene which encodes adenylate cyclase and these cells contain no measurable amounts of cAMP. When M-factor is added to a growing h+ cyr1- strain it causes a transient G1 arrest of cell division, transcription of mat1-Pm, and elongation of the cells to form shmoos. The second mutant contains the temperature-sensitive pat1-114 allele. At 30 degrees C this mutant was previously shown not only to bypass the nutritional signal but also to stop growing in a state derepressed for pheromone-controlled functions. We now report that an h+ ...

1994-01-01

202

Mitochondrial genetic damage induced in yeast by a photoactivated furocoumarin in combination with ethidium bromide or ultraviolet light

Management of industrial solid wastes in Alexandria, Egypt

Ethidium bromide (EB) and ultraviolet light (UV) in combination are known to produce a synergistic induction of 'petite' mutants in yeast. Two other agents were combined with EB, 3-Carbethoxypsoralene (3 CPs) activated by 365 nm light or #gamma# rays. EB in combination with 3 CPs also resulted in an enhanced production of 'petite' mutants. After the photoaddition of 3 CPs in exponential phase cells, recovery of the 'petite' mutation during dark liquid holding was inhibited by the presence of EB producing an enhanced number of 'petite' mutants. The behavior of mitochondrial antibiotic resistance markers after individual and combined treatments with EB and 3 CPs indicates a random loss of markers after EB and a preferential loss of a certain region for the 3 CPs photoaddition. The combination of the two agents leads to an additivity of total drug marker losses rather than a synergistic loss. The combination of EB with #gamma# rays produced no enhancement in 'petite' ...

203

Mineral biotechnology. Microbial aspects of mineral beneficiation, metal extraction, and environmental control

This paper presents a summary of the first phase of the EPA project, which encompasses surveys of residues from industrial sources in Alexandria. Studies to date indicate that wastes from various industries can be recovered economically. Wastes such as tin cans, glass, wastepaper, and food residues from processing of fruits, starch, and beer are examples of reusable industrial wastes in Egypt. The results of experimental studies for reuse of residues from oil refining, starch and yeast processing, and steel pickling are presented. Spent clay from edible oil refining is currently discarded, causing both handling and disposal problems. This clay contains as much as 40% oil; 90% can be recovered by extraction. The recovered oil can be successfully used in soap production, and the spent clay can be reused in oil bleaching. Other examples include starch and yeast wastes, which can be used for animal feed, and spent pickling liquor, which can be used ...

1983-03-01

204

Mechanism of biodegradation of paraquat by Lipomyces starkeyi

Papers in this book illustrate the utility of mineral biotechnology with respect to biobeneficiation, bioleaching, bioremediation and biomineralization. Papers of particular interest to the coal industry include: depression of pyrite flotation by yeast and bacteris (S.K. Kawatra and T.C. Eisele); desulfurization of coal by microbial flotation in a semicontinuous system (T. Nagaoka and others); biochemical removal of HAP precursors from coal - INEEL slurry column testing (K.S. Noah and G.J. Olson); microorganisms, biotechnology and acid rock drainage - emphasis on passive-biological control and treatment methods (N. Kuyucak); and utility of bioreagents in mineral processing (P. Somasundaran and others).

2001-07-01

205

Mechanism of biodegradation of paraquat by Lipomyces starkeyi

The biodegradation of ring-/sup 14/C- and methyl-/sup 14/C-labeled paraquat by the soil yeast Lipomyces starkeyi was studied in vitro. It was found that the degradation of paraquat (acting as a sole source of culture nitrogen) resulted in the accumulation in the extracellular medium of radiolabeled acetic acid. The culture also evolved radiolabeled CO/sub 2/. The results suggest that the degradation of paraquat by L. starkeyi is associated with the integrity of the cell wall and that disruption or removal of the wall results in a complete loss of degradative capability. A mechanism for the degradation of paraquat by this organism is postulated.

1985-05-01

206

Antifungal activity of Glycyrrhiza glabra extracts and its active constituent glabridin

The biodegradation of ring-"1"4C- and methyl-"1"4C-labeled paraquat by the soil yeast Lipomyces starkeyi was studied in vitro. It was found that the degradation of paraquat (acting as a sole source of culture nitrogen) resulted in the accumulation in the extracellular medium of radiolabeled acetic acid. The culture also evolved radiolabeled CO_2. The results suggest that the degradation of paraquat by L. starkeyi is associated with the integrity of the cell wall and that disruption or removal of the wall results in a complete loss of degradative capability. A mechanism for the degradation of paraquat by this organism is postulated.

207

British Library Electronic Table of Contents (United Kingdom)

Glabridin, an active constituent of Glycyrrhiza glabra roots, was found to be active against both yeast and filamentous fungi. Glabridin also showed resistance modifying activity against drug resistant mutants of Candida albicans at a minimum inhibitory concentration of 31.25-250 g/mL. Although the compound was reported earlier to be active against Candida albicans, but this is the first report of its activity against drug resistant mutants. Copyright Copyright 2009 John Wiley & Sons, Ltd.

2009-01-01

208

Antifungal activity of the extracts and neolignans from Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck

Proteomic analysis of the shistosome tegument and its surface membranes

Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck (Piperaceae) is a medicinal plant traditionally used in Brazil to treat infectious diseases. The extracts obtained from the leaves of P. regnellii were investigated for their antifungal activities against the yeasts Candida albicans, C. krusei, C. parapsilosis, and C. tropicalis. The EtOAc extract presented gnificant activity against Candida albicans with MIC at 125 {mu}g mL{sup -1}, and a moderate activity against both C. krusei and C. parapsilosis with MIC at 500 {mu}g mL{sup -1}. Candida tropicalis was not inhibited by this extract at concentrations as high as 1000 {mu}g mL{sup -1}. Based on these findings, the EtOAc extract was fractionated by silica gel column chromatography into nine fractions. The hexane and CHCl{sub 3} fractions showed varied levels of antifungal activity against all test yeasts. Further column chromatography separation of the hexane fraction afforded the pure ...

2005-11-15

209

Scientific Electronic Library Online (English)

Abstract in english The tegument surface of the adult schistosome, bounded by a normal plasma membrane overlain by a secreted membranocalyx, holds the key to understanding how schistosomes evade host immune responses. Recent advances in mass spectrometry (MS), and the sequencing of the Schistosoma mansoni transcriptome/genome, have facilitated schistosome proteomics. We detached the tegument from the worm body and enriched its surface membranes by differential extraction, before subjecting t (more) he preparation to liquid chromatography-based proteomics to identify its constituents. The most exposed proteins on live worms were labelled with impearmeant biotinylation reagents, and we also developed methods to isolate the membranocalyx for analysis. We identified transporters for sugars, amino acids, inorganic ions and water, which confirm the importance of the tegument plasma membrane in nutrient acquisition and solute balance. Enzymes, including phosphohydrolases, ...

2006-10-01

210

Proteomic Analysis of Proteins Secreted by HepG2 Cells Treated with Butyl Benzyl Phthalate

British Library Electronic Table of Contents (United Kingdom)

Proteomic changes in proteins secreted by human hepatocellular carcinomas (HepG2) cells exposed to butyl benzyl phthalate (BBP) were evaluated. HepG2 cells were treated with three different concentrations of BBP (0, 10, or 25 ?M) for 24 or 48 h. Following incubation, the cells were subjected to proteomic analysis using two different pI ranges (4-7 and 6-9) and large-size two-dimensional gel electrophoresis. Results showed resolution of a total of 2776 protein spots. Of these, 29, including 19 upregulated and 10 downregulated proteins, were identified by electrospray ionization-mass spectrometry-mass spectrometry (ESI-MS/MS). Among these, the identities of cystatin C, Rho guanine nucleotide dissociation inhibitor, gelsolin, DEK protein, Raf kinase inhibitory protein, triose phosphate ...

2010-01-01

211

Actual and future strategies in interdisciplinary treatment of medulloblastomas, supratentorial PNET and intracranial germ cell tumors in childhood

Use of integrin-linked kinase to extend function of encapsulated pancreatic tissue

Methods: Systemic irradiation of neuroaxis is an essential part in the management of medulloblastoma, stPNET and intracranial germ cell tumors. The introduction of quality assurance programs in radiooncology assures a precise radiotherapy of target volumes and is a prerequisite to improve survival. Results: Hyperfractionated radiotherapy has the potential of increasing dose to tumor more safely without increasing the risk for late adverse effects. Pilot studies revealed excellent tumor control in medulloblastoma with acceptable acute toxicity and a long-term survival of up to 96%. In medulloblastoma stereotactic radiation techniques reveal an acceptable toxicity and promising results in tumor control in recurrent disease or as primary treatment. They are now part of future treatment protocols in case of persisting residual tumor. Radiotherapy alone in pure germinoma is continuously yielding high cure rates. In secreting germ cell tumors cisplatin containing ...

2001-09-01

212

The early days of parathyroid surgery.

We have studied the impact of overexpression of an intracellular signaling protein, integrin-linked kinase (ILK), on the survival and function of encapsulated islet tissue used for the treatment of type 1 diabetes. The dimensions of the encapsulated tissue can impact the stresses placed on the tissue and ILK overexpression shows the ability to extend function of dissociated cells as well as intact islets. These results suggest that lost cell-extracellular matrix interactions in cell encapsulation systems can lead to decreased insulin secretion and ILK signaling is a target to overcome this phenomenon. (communication)

2010-12-15

213

Monoclonal antibodies to antigens on human neutrophils, activated T lymphocytes, and acute leukemia blast cells

The parathyroid endocrine glands, usually four in number and about the size of a split pea, lie on either side of the posterior aspect of the thyroid gland. Occasionally one or more of these structures are found in an aberrant position in the neck or even in the superior mediastinum. The parathyroid secretion, parathormone, regulates the level of serum calcium within sharply defined limits. Lack of parathormone, with lowering of the serum calcium, results in hyper-irritability of skeletal muscle, the clinical condition known as tetany. This is encountered following inadvertent removal or bruising of the parathyroid glands at thyroidectomy. PMID:21488463

2011-03-01

214

Adrenocortical (dys)function in septic shock - A sick euadrenal state

The authors describe the production of two mouse hybridomas secreting monoclonal antibodies to antigenic determinants of the surface membranes of human neutrophils, activated T lymphocytes, and acute leukemic blast cells. The degree of lymphocyte stimulation was estimated from incorporation of /sup 3/H-thymidine with parallel microculture. Monoclonal antibodies of supernatants of hybridoma cultures shown here reacted in both immunofluorescence test and cytotoxicity test with surface membrane antigens on the majority of neutrophils and PHA-activated peripheral blood lymphocytes from healthy subjects, but did not give positive reactions with unactivated lymphocytes, adherent monocytes, erythrocytes, and alloantigen-stimulated lymphocytes.

1987-11-01

215

British Library Electronic Table of Contents (United Kingdom)

A central feature of the endocrine pathophysiology of septic shock is thought to be the existence of adrenal dysfunction. Based on changes in glucocorticoid secretion and responsiveness, protein binding, and activity. These changes have been described by the terms "Relative Adrenal Insufficiency" (RAI), or "Critical Illness Related Corticosteroid Insufficiency" (CIRCI), and form part of the rationale for trials of glucocorticoid treatment in septic shock. Diagnostic criteria for these conditions have been based on plasma cortisol profiles and have proven notoriously difficult to establish. The uncertainty in this area arises from the inability of current tests to clearly identify who is truly glucocorticoid "deficient" at a cellular level, and hence who requires supplemental glucocorticoid...

2011-01-01

216

On-chip-network Protocol for Efficient Network Utilization

Flexible, Extensible, and Efficient VANET Authentication

Full Text Available

2010-01-01

217

Environmental Effects and Energy Efficiency in Building Design: A Green Building Approach. Part 3. Design Case Studies.

Full Text Available

2009-12-01

218

Efficiency of the second scintillator plane of the ANKE forward detector

Contents: Introduction; Design Criteria for a Green Building; Comparison of Energy Efficiency Measures; Whole House Energy Efficiency; Built Examples of Energy Efficient Houses; and Product Energy of Building Elements.

1993-01-01

219

Determination of the ANKE forward detector MWPC efficiency

No abstract prepared.

2002-03-01

220

First-Order Altitude Effects on the Cruise Efficiency of Subsonic Transport Aircraft

No abstract prepared.

2002-03-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

221

Wiley::The Wiley Encyclopedia of Packaging Technology, 3rd Edition

Aircraft fuel efficiency is a function of many different parameters, including characteristics of

2011-01-01

222

The antimicrobial efficacy of a silver alginate dressing against a broad spectrum of clinically relevant wound isolates

...The+Wiley+Encyclopedia+of+Packaging+Technology%2C+3rd+Edition FO21+Food+Packaging Wiley::The Wiley Encyclopedia of Packaging Technology, 3rd Edition WILEY ...US | HELP Home / Chemistry / Food Science & Technology / Food Processing, Production & Manufacture / Food Packaging / The Wiley Encyclopedia of Packaging Technology, 3rd Edition ...Manufacture Sensory Science Food Biotechnology Food Chemistry Food Engineering Related Titles Food Packaging Brewing Yeast and Fermentation by Chris Boulton, David Quain Food ...Editor) Journal of Food Process Engineering Journal of Food Processing and Preservation Packaging Research in Food Product Design and Development by Howard R. ...

223

British Library Electronic Table of Contents (United Kingdom)

Wound dressings impregnated with silver have a role to play in aiding to reduce both the dressing and wound microbial bioburden. It is therefore imperative that antimicrobial wound dressings have efficacy on a broad range of clinical significant microorganisms. Accordingly, this study aimed to determine the antimicrobial efficacy of a silver alginate dressing against 115 wound isolates that had been isolated routinely from patients at West Virginia University Hospital. Standardised corrected zones of inhibition (CZOIs) were performed on all clinical isolates. It was found that the silver alginate dressing was able to inhibit the growth of all microorganisms tested. In particular, the silver alginate dressing inhibited the growth of Candida albicans and yeasts with CZOI of 3-115 mm. All met...

2011-01-01

224

The DFNA5 gene, responsible for hearing loss and involved in cancer, encodes a novel apoptosis-inducing protein

British Library Electronic Table of Contents (United Kingdom)

DFNA5 was first identified as a gene causing autosomal dominant hearing loss (HL). Different mutations have been found, all exerting a highly specific gain-of-function effect, in which skipping of exon 8 causes the HL. Later reports revealed the involvement of the gene in different types of cancer. Epigenetic silencing of DFNA5 in a large percentage of gastric, colorectal and breast tumors and p53-dependent transcriptional activity have been reported, concluding that DFNA5 acts as a tumor suppressor gene in different frequent types of cancer. Despite these data, the molecular function of DFNA5 has not been investigated properly. Previous transfection studies with mutant DFNA5 in yeast and in mammalian cells showed a toxic effect of the mutant protein, which was not seen after transfection ...

2011-01-01

225

Synthesis and study of the antifungal activity of new mono- and disubstituted derivatives of a genetically engineered polyene antibiotic 28,29-didehydronystatin A1 (S44HP)

British Library Electronic Table of Contents (United Kingdom)

Mono- and disubstituted novel derivatives of the heptaene nystatin analog 28,29-didehydronystatin A1 (S44HP, 1) were obtained by chemical modification of the exocyclic C-16 carboxyl and/or an amino group of mycosamine moiety. The strategy of preparation of mono- and double-modified polyene macrolides was based on the use of intermediate hydrophobic N-Fmoc (9-fluorenylmethoxycarbonyl) derivatives that facilitated the procedures of isolation and purification of new compounds. The antifungal activity of the new derivatives was first tested in vitro against yeasts and filamentous fungi, allowing the selection of the most active compounds that were subsequently tested for acute toxicity in mice. 2-(N,N-dimethylamino)ethylamide of 1 (2) and 2-(N,N-dimethylamino)ethylamide of N-fructopyranosyl-28...

2010-01-01

226

Loss of cell components during rehydration of dried Rhodotorula glutinis and its implications for lead uptake

British Library Electronic Table of Contents (United Kingdom)

Abstract Microbial cells are routinely dried and ground before they are used in metal biosorption studies. In this work, a metal biosorbent was prepared by drying biomass of the yeast Rhodotorula glutinis in an oven at 70C for 24-h followed by grinding. Two forms of the prepared biosorbent particles, washed and unwashed, were examined for their ability to remove lead from solution. It was found that the unwashed biosorbent exhibited higher lead uptake than the washed biosorbent. Analysis of the supernatant of washed cells incubated in water and that of unwashed cells incubated in lead solution revealed the presence of protein, carbohydrates, organic acids and inorganic phosphate. Overall, the washed and unwashed cells leached, respectively, 14.5 and 13.4% of their initial dry weight (100-m...

2011-01-01

227

In vitro effects of medium tonicity, nutrient concentration, and free chlorine content on Acanthamoeba

British Library Electronic Table of Contents (United Kingdom)

Background: The environment preferred by Acanthamoeba trophozoites and the mechanism by which the amebae enters the cornea are not yet fully understood. A better understanding of the pathogenesis of this disease may help with prevention and treatment. Purpose: To define the preferred environments for Acanthamoeba survival and proliferation in vitro by examining the effect of tonicity, nutrient concentration, and free chlorine content on Acanthamoeba. Materials and methods: Human corneal isolates of Acanthamoeba castellanii and Acanthamoeba polyphaga trophozoites were cultured at 22^oC (room temperature) in PYG (peptone-yeast extract-glucose) medium. The effect of tonicity on amebae was determined by incubating trophozoites in sodium chloride solutions in concentrations ranging from 0% to 1...

2011-01-01

228

Human type I pituitary adenylate cyclase activating polypeptide receptor (ADCYAP1R): Localization to chromosome band 7p14 and integration into the cytogenetic, physical, and genetic map of chromosome 7

Enhancement of bioleaching of a spent Ni/Mo hydroprocessing catalyst by Penicillium simplicissimum

The gene encoding the human type I pituitary adenylate cyclase activating polypeptide receptor (ADCYAP1R1) was mapped to chromosome 7 by PCR analysis of genomic DNA from a human/rodent somatic cell hybrid mapping panel. This assignment was confirmed and the gene localized to chromosome band 7p14 by fluorescence in situ hybridization. A yeast artificial chromosome containing ADCYAP1R1 was identified in the CEPH {open_quotes}B{close_quotes} Mega-YAC library. This YAC includes two highly polymorphic dinucleotide repeat sequences that will facilitate genetic studies of the contribution of ADCYAP1R1 in disease states of the central nervous and neuroendocrine systems. 13 refs., 1 fig.

1994-10-01

229

British Library Electronic Table of Contents (United Kingdom)

Statistically based experimental designs were applied to screen and optimize the bioleaching of spent hydrocracking catalyst by Penicillium simplicissimum. Eleven factors were examined for their significance on bioleaching using a Plackett-Burman factorial design. Four significant variables (pulp density, sucrose, NaNO"3, and yeast extract concentrations) were selected for the optimization studies. The combined effect of these variables on metal bioleaching was studied using a central composite design (CCD). Second-order polynomials were established to identify the relationship between the recovery percent of the metals and the four significant variables. The optimal values of the variables for maximum metals bioleaching were as follows: pulp density (4.0%, w/v), sucrose (90g/L), NaNO"3 (2...

2011-01-01

230

Curcumin Binding to DNA and RNA

British Library Electronic Table of Contents (United Kingdom)

Curcumin, the yellow pigment from the rhizoma of Curcuma longa, is a widely studied phytochemical with a variety of biological activities. The ongoing research and clinical trials have proved that this natural phenolic compound has great and diverse pharmacological potencies. Beside its effective antioxidant, antiinflammatory, and antimicrobial/antiviral properties, curcumin is also considered as a cancer chemopreventive agent. While the antioxidant activity of curcumin is well documented, its interaction with DNA and RNA is not fully investigated. This study was designed to examine the interactions of curcumin with calf thymus DNA and yeast RNA in aqueous solution at physiological conditions, using constant DNA and RNA concentration (6.25?mM) and various curcumin/polynucleotide (phosphate...

2009-01-01

231

Comparison of media and methods for detecting and enumerating Listeria monocytogenes in refrigerated cabbage.

Chromosomal localization and structure of the human type II IMP dehydrogenase gene

Direct plating, selective enrichment, and cold enrichment followed by secondary selective enrichment procedures were compared for detecting and enumerating Listeria monocytogenes in chopped cabbage stored at 5 degrees C for up to 64 days. Addition of Fe3+ to solid media enhanced detection of the organism. Cold enrichment (5 degrees C) in nutrient broth and brain heart infusion broth followed by secondary enrichment (48 h, 30 degrees C) in Trypticase soy-yeast extract-antibiotic broth and thiocyanate-nalidixic acid broth and plating on selective agar media (Doyle and Schoeni selective enrichment agar [minus acriflavin hydrochloride, supplemented with 5 micrograms of Fe3+/ml] and McBride Listeria agar) resulted in the detection of highest populations. PMID:3111369

1987-05-01

232

Biological treatment of wine of distilleries

We determined the chromosomal localization and structure of the gene encoding human type II inosine 5{prime}-monophosphate dehydrogenase (IMPDH, EC 1.1.1.205), an enzyme associated with cellular proliferation, malignant transformation, and differentiation. Using polymerase chain reaction (PCR) primers specific for type II IMPDH, we screened a panel of human-Chinese hamster cell somatic hybrids and a separate deletion panel of chromosome 3 hybrids and localized the gene to 3p21.1{yields}p24.2. Two overlapping yeast artificial chromosome clones containing the full gene for type II IMPDH were isolated and a physical map of 117 kb of human genomic DNA in this region of chromosome 3 was constructed. The gene for type II IMPDH was localized and oriented on this map and found to span no more than 12.5 kb.

1994-05-01

233

Bioconvertion of spent cellulose sausage casings

The potential of the yeast Candida tropicalis and Candida guillermondii was evaluated and an isolated partnership of microorganisms of waters of the Medellin River, conformed by two bacteria and one leavening, to degrade the content of organic matter present in wine produced by the factory of Licores and Alcoholes de Antioquia (FLA) in aerobic process with biomass production. For each one of the microorganisms in study this capacity of removal in units of chemical demand of oxygen was quantified (CDO); in addition, parameters were analyzed such as yield of the biomass in relation to the removed CDO and to total reducing sugars (TRS) consumed, time of fermentation and speed of growth different dilutions from wine. Also the possible inhibition was analyzed that the present phenolic compounds in this wine can cause in the biological process of degradation.

234

British Library Electronic Table of Contents (United Kingdom)

Cellulose sausage cellulose casings are used extensively in the manufacture of sausages in meat packaging. After stripping the meat, spent casings mainly contain cellulose and residual meat juice with salt, nitrate and nitrite. Disposal of spent sausage casings has serious economic and environmental concerns for the sausage industry. This work describes bioconversion of spent cellulose casings (SCC) into enzymes, lactic acid and ethanol by using cellulolytic fungi, lactobacillus and yeasts. The solid substrate cultivation (SSC) of Trichoderma reesei RUT C-30 on SCC and blends gave a maximum of 152 filter paper cellulase (FPase) activity and about 100 carboxymethylcellulase activity (CMCase)/g dry weight substrate. The SSC produced enzyme-rich casing with 50 FPase when directly mixed as suc...

2008-01-01

235

Antimicrobial silver-montmorillonite nanoparticles to prolong the shelf life of fresh fruit salad

British Library Electronic Table of Contents (United Kingdom)

In this work, silver-montmorillonite (Ag-MMT) antimicrobial nanoparticles have been obtained by allowing silver ions from nitrate solutions to replace the Na^+ of natural montmorillonite and then to be reduced by a thermal treatment. Ag-MMT were used as active antimicrobial compounds to improve the shelf life of fresh fruit salad. In order to assess their influence on product shelf life, sensorial and microbiological quality has been monitored during the storage. The microbiological quality was determined by monitoring the principal spoilage microorganisms (mesophilic and psychrotrophic bacteria, coliforms, lactic acid bacteria, yeasts and molds). Additionally, the evolution of sensorial quality was assessed by monitoring color, odor, firmness and product overall quality. The Ag-MMT nanopa...

2011-01-01

236

Amphiphysin (Amph) maps to the proximal region of mouse chromosome 13

Aerobic Decolorization and Detoxification of a Disperse Dye in Textile Effluent by a New Isolate of Bacillus sp.

Amphiphysin is a protein concentrated in neuronal synapses and peripherally associated with neurotransmitter vesicles. It is expressed in many neurons of the central and peripheral nervous systems, in the adrenal medulla, in the anterior and posterior pituitary, in cell lines of the endocrine pancreas, and in spermatocytes. Its subcellular localization and tissue distribution indicate a potential involvement in mechanisms of regulated exocytosis. A role in the dynamic organization of the membrane-associated cytoskeleton is suggested by structural homology to the products of two yeast genes, RVS161 and RVS167, whose mutation results in an abnormal actin distribution, disturbs budding morphology, and impairs cell entry into stationary phase. Limited stretches of sequence similarity, including an SH3 domain, are also shared with other actin-binding proteins. Amphiphysin is the dominant autoantigen in paraneoplastic Stiff-Man syndrome, a neurological autoimmune ...

1995-07-20

237

British Library Electronic Table of Contents (United Kingdom)

A number of aerobic species capable of decolorizing some of the dyes in a textile mill effluent were isolated. One of the isolates was able to decolorize Terasil black dye under aerobic conditions in the presence of an exogenous carbon source after 5 days. Glucose or starch (%1 ea) are essential for decolorization but the process proceeds faster in the presence of 0.5% yeast extract. Results of the BOD5 show that the untreated effluent samples have a low BOD value, whereas treated samples show an initial increase in BOD up to 15 days followed by a decrease after 20 days. FT-IR and GC-MS data also reveal that the initial components in the untreated effluent disappear after 20 days of treatment, confirming biodegradation of the dye. Phytotoxicity tests on the untreated effluent samples using...

2006-01-01

238

A lytic enzyme cocktail from Streptomyces sp. B578 for the control of lactic and acetic acid bacteria in wine

British Library Electronic Table of Contents (United Kingdom)

Beside yeasts, lactic acid bacteria (LAB) are the most abundant microbes in must during vinification. Whereas Oenococcos oeni is commercially used as a starter culture for the biological acid reduction in wines, other species are responsible for different types of wine spoilage. Members of the genera Pediococcus, Weissella, Leuconostoc, and Lactobacillus are producers of exopolysaccharide slimes, biogenic amines, acetic acid, and other off-flavors. In order to control microbial growth, different procedures such as heating of must and addition of sulfite or lysozyme from egg white are generally applied. Yet, because of health risks, the application of sulfite should be reduced and lysozyme is not effective against all LAB. In this study, we describe exoenzymes from a Streptomyces sp. strain...

2009-01-01

239

Vaginal Matrix Metalloproteinase Levels in Pregnant Women With Bacterial Vaginosis

British Library Electronic Table of Contents (United Kingdom)

Objective To compare matrix metalloproteinase (MMP)-8 and MMP-9 levels in the vaginal secretions of pregnant women with or without asymptomatic bacterial vaginosis (BV). Methods In this study, vaginal levels and molecular forms of MMP-8 and MMP-9 were studied in 36 pregnant women between 28 and 34 weeks of gestation with asymptomatic BV and 41 pregnant women, matched for gestational age, without BV. Results Vaginal MMP-8 concentrations were significantly higher (P = .023) in BV-positive women. There was no significant difference in MMP-9 levels between healthy pregnant controls and BV-positive pregnant women. The presence of MMP-8 was confirmed by a 38-kd band on Western blots. Conclusions Our findings show that BV is associated with increased levels of MMP-8 in vaginal fluid. Increased pr...

2006-01-01

240

The hidden secrets of the E-center in Si and Ge

Survey o methods for secure connection to the internet; Internet tono anzenna setsuzoku hoshiki no genjo

The group- V vacancy pair, the so-called E-center, has recently been demonstrated to have, both in Si and Ge, more complicated energy-level schemes in the energy gap than were previously assumed. The E-center in silicon has, in addition to its well-established single-acceptor level in the upper half of the band gap, also a donor level in the lower half of the band gap; this donor level has lain hidden for more than 40 years. The E-center in Ge has an even more complicated level scheme as it induces, in addition to two levels analogous to those found in Si, also a double-acceptor level in the upper half of the band gap. Thus the E-center in Si can exist in three charge states and the E-center in Ge in four.

2007-12-15

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

241

Secure Direct Communication Based on Non-Orthogonal Entangled Pairs and Local Measurement

This paper describes a study of a security method of protecting inside network computers against outside miscreants and unwelcome visitors and a control method when these computers are connected with the Internet. In the present Internet, a method to encipher all data cannot be used, so that it is necessary to utilize PEM (Privacy Enhanced Mail) capable of the encipherment and conversion of secret information. For preventing miscreant access by eavesdropping password, one-time password is effective. The most cost-effective method is a firewall system. This system lies between the outside and inside network. By limiting computers that directly communicate with the Internet, control is centralized and inside network Security is protected. If the security of firewall systems is strictly controlled under correct setting, security within the network can be secured even in open networks such as the Internet. 35 refs., 3 figs.

1994-04-01

242

Purpurin is a key molecule for cell differentiation during the early development of zebrafish retina

We propose a quantum secure direct communication scheme based on non-orthogonal entangled pairs and local measurement. In this scheme, we use eight non-orthogonal entangled pairs to act as quantum channels. Due to the non-orthogonality of the quantum channels, the present protocol can availably prohibit from all kinds of valid eavesdropping and acquire a secure quantum channel. By local measurement, the sender acquires a secret random sequence. The process of encoding on the random sequence is identical to the one in one-time-pad. So the present protocol is secure. Even for a highly lossy channel, our scheme is also valid. The scheme is feasible with present-day techniques.

2008-12-01

243

British Library Electronic Table of Contents (United Kingdom)

Recently, we cloned purpurin cDNA as an upregulated gene in the axotomized fish retina. The retina-specific protein was secreted from photoreceptors to ganglion cell layer during an early stage of optic nerve regeneration in zebrafish retina. The purpurin worked as a trigger molecule for axonal regrowth in adult injured fish retina. During zebrafish development, purpurin mRNA first appeared in ventral retina at 2 days post-fertilization (dpf) and spread out to the outer nuclear layer at 3 dpf. Here, we investigated the role of purpurin for zebrafish retinal development using morpholino gene knockdown technique. Injection of purpurin morpholino into the 1-2 cell stage of embryos significantly inhibited the transcriptional and translational expression of purpurin at 3 dpf. In the purpurin mo...

2009-01-01

244

PKH26 as a fluorescent label for live human umbilical mesenchymal stem cells

British Library Electronic Table of Contents (United Kingdom)

To determine whether PKH26 labeling affects the morphologies, phenotypes, proliferation, and secretion abilities of human umbilical mesenchymal stromal cells (HUMSCs) were investigated. Isolated HUMSCs were labeled with PKH26, and cell morphology was observed under microscope. Cell cycle, apoptotic cell death, expression of PKH26, and the proliferation rate were evaluated. Additionally, fluorescence intensity of PKH26 labeling at different passage times was quantified. There were no detectable differences in cell morphology, cell growth, and proliferation rate after PKH26 labeling. In addition, fluorescence intensity of PKH26 labeling was gradually reduced with increase of the passage times. The PKH26 labeling disappeared after passage six times. In summary, PKH26 labeling is a safe and ef...

2011-01-01

245

Nickel induced structural and functional alterations in mouse Leydig cells in vitro

British Library Electronic Table of Contents (United Kingdom)

The present study was aimed at investigating effects of nickel (NiCl2) on secretion of testosterone (T), cell viability, ultrastructure and apoptosis in mouse Leydig cells. Testosterone release was measured after 48h of culture with 15.67, 31.25, 62.5, 125, 250, 500 and 1000mmol/L NiCl2 or without NiCl2 using radioimmunoassay. Cell viability was assessed by a MTT (metabolic activity assay). Quantification of apoptotic cells was performed using TUNEL assay and the ultrastructural changes were analyzed using transmission electron microscopy. The viability was decreased after addition of >=250mmol/L NiCl2. A concentration-dependent depression of T production was observed. The percentage of apoptotic cells was significantly increased only after addition of 125, 250 and 1000mmol/L NiCl2. After ...

2011-01-01

246

Monoclonal antibodies: new agents for cancer detection and targeted therapy

Molecular biology of the renin-angiotensin system

Antibodies directed against markers on cancer cells are gaining in importance for the purpose of targeting diagnostic and therapeutic agents. In the past, this approach has had very limited success principally because the classical methods for producing antibodies from blood serum of animals immunized with cancer cells or extracts were unsatisfactory. The situation has changed dramatically since 1975 following the design of procedures for 'immortalizing' antibody-producing cells (lymphocytes) by fusing them with cultured myeloma cells to form hybridomas which continuously secrete antibodies. Since these hybridomas produce antibodies coded for by a single antibody-producing cell, the antibodies are called monoclonal. Building on these advances in biomedical research, it is now possible to reproducibly manufacture monoclonal antibodies on a scale suitable for use in cancer detection and therapy.

247

Impact of antipsychotics on cytokine production in-vitro

This paper reviews the molecular biology of the renin-angiotensin system. The renin gene structure is analyzed in detail, including an examination of the putative regulatory regions. The combined action of these regulatory sequences would result in the complex, tissue-specific expression and regulation observed in vivo. The expression of the tissue renin-angiotensin systems, which may have important physiological functions, is also described. In addition, the pathway of renin biosynthesis and secretion is reviewed. This includes speculation on the fate of circulating prorenin and the physiological role of multiple renin forms and secretory pathways. The molecular approaches described in this paper have greatly advanced our knowledge of the biology of the renin-angiotensin system. Future studies using these and other approaches should provide further insight into this complex system.

1988-10-01

248

British Library Electronic Table of Contents (United Kingdom)

Objective: A growing body of data from genetic, immunological and clinical studies indicates an involvement of the immune system in the pathophysiology of schizophrenia and suggests that the modulation of the cytokine system by antipsychotics may be one cause for the improvement of psychotic symptoms. However, the influence of the typical antipsychotics chlorpromazine and haloperidol, and the effect of typical and atypical antipsychotics on the TSST-1-stimulated blood cell secretion of cytokines, and specifically the interleukin (IL)-17 production have not been studied so far, although IL-17 is a leading pro-inflammatory cytokine. Method: We measured levels of IL-1@b, IL-2, IL-4, IL-6, IL-17 and tumor necrosis factor-@a (TNF-@a) in stimulated blood of 10 healthy female subjects in a whole ...

2011-01-01

249

Forming the COUNCIL Based Clusters in Securing Wireless Ad Hoc Networks

Food reward functions as affected by obesity and bariatric surgery

In cluster-based routing protocol (CBRP), two-level hierarchical structure is successfully used to reduce over-flooding in wireless Ad Hoc networks. As it is vulnerable to a single point of failure, we propose a new adaptive distributed threshold scheme to replace the cluster head by a group of cluster heads within each cluster, called COUNCIL, and distribute the service of single cluster head to multiple cluster heads using (k,n) threshold secret sharing scheme. An Ad Hoc network formed by COUNCIL based clusters can work correctly when the number of compromised cluster heads is smaller than k. To implement this adaptive threshold scheme in wireless Ad Hoc Networks, membership of the clusters should be defined in an adaptive way. In this paper, we mainly discuss our algorithm for forming COUNCIL based clusters using the concept of dominating set from graph theory.

2010-01-01

250

British Library Electronic Table of Contents (United Kingdom)

Roux-en-Y gastric bypass surgery (RYGB) remains to be the most effective long-term treatment for obesity and its associated comorbidities, but the specific mechanisms involved remain elusive. Because RYGB patients appear to no longer be preoccupied with thoughts about food and are satisfied with much smaller meals and calorically dilute foods, brain reward mechanisms could be involved. Just as obesity can produce maladaptive alterations in reward functions, reversal of obesity by RYGB could normalize these changes or even further reset the food reward system through changes in gut hormone secretion, aversive conditioning and/or secondary effects of weight loss. Future studies with longitudinal assessments of reward behaviors and their underlying neural circuits before and after surgery wil...

2011-01-01

251

Exploration of the hypothalamic?pituitary?adrenal function as a tool to evaluate animal welfare

British Library Electronic Table of Contents (United Kingdom)

Measuring HPA axis activity is the standard approach to the study of stress and welfare in farm animals. Although the reference technique is the use of blood plasma to measure glucocorticoid hormones (cortisol or corticosterone), several alternative methods such as the measurement of corticosteroids in saliva, urine or faeces have been developed to overcome the stress induced by blood sampling itself. In chronic stress situations, as is frequently the case in studies about farm animal welfare, hormonal secretions are usually unchanged but dynamic testing allows the demonstration of functional changes at several levels of the system, including the sensitization of the adrenal cortex to ACTH and the resistance of the axis to feedback inhibition by corticosteroids (dexamethasone suppression t...

2007-01-01

252

Distributions of /sup 35/S-sulfate and /sup 3/H-glucosamine in the angular region of the hamster: light and electron microscopic autoradiography

Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola

The distribution of /sup 35/S-sulfate and /sup 3/H-glucosamine in the angular region of the hamster was studied by light and electron microscopic autoradiography following intraperitoneal injection of these compounds to hamsters. Exposed silver grains of /sup 35/S-sulfate were concentrated in the trabecular meshwork, sclera, and cornea, and grains of /sup 3/H-glucosamine were localized in the trabecular region. The radioactivity of both isotopes was observed in the Golgi apparatuses of the endothelial cells of the angular aqueous plexus and the trabecular meshwork. The grains were noted over the entire cytoplasm, except for the nucleus, and then were incorporated into the amorphous substance and collagen fibers in the region adjacent to the angular aqueous sinus. These results suggest that endothelial cells in the angular region synthesize and secrete the sulfated glycosaminoglycans and hyaluronic acid.

1983-06-01

253

British Library Electronic Table of Contents (United Kingdom)

Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobacterium-mediated transformation. Phytase expression in transgenic plant was enhanced with a synthetic phytase gene according to the Brassica codon usage and an endoplasmic reticulum (ER) retention signal KDEL that confers an ER accumulation of the recombinant phytase. Secretion of the phytase to the extracellular fluid was also established by the use of the tobacco PR-S signal peptide. Phytase accumulation in mature seed accounted for 2.6% of the total soluble proteins. The enzy...

2006-01-01

254

Brain glucose sensing and neural regulation of insulin and glucagon secretion

British Library Electronic Table of Contents (United Kingdom)

Glucose homeostasis requires the tight regulation of glucose utilization by liver, muscle and white or brown fat, and glucose production and release in the blood by liver. The major goal of maintaining glycemia at -5 mM is to ensure a sufficient flux of glucose to the brain, which depends mostly on this nutrient as a source of metabolic energy. This homeostatic process is controlled by hormones, mainly glucagon and insulin, and by autonomic nervous activities that control the metabolic state of liver, muscle and fat tissue but also the secretory activity of the endocrine pancreas. Activation or inhibition of the sympathetic or parasympathetic branches of the autonomic nervous systems are controlled by glucose-excited or glucose-inhibited neurons located at different anatomical sites, mainl...

2011-01-01

255

Apelin, diabetes, and obesity

British Library Electronic Table of Contents (United Kingdom)

Apelin is a peptide known as the ligand of the G-protein-coupled receptor APJ. Several active apelin forms exist such as apelin-36, apelin-17, apelin-13, and the pyroglutamated form of apelin-13. Apelin and APJ are expressed in the central nervous system, particularly in the hypothalamus and in many peripheral tissues. Apelin has been shown to be involved in the regulation of cardiovascular and fluid homeostasis, food intake, cell proliferation, and angiogenesis. In addition to be an ubiquitous peptide, apelin is also produced and secreted by adipocytes and thus considered as an adipokine. This has opened a new field of investigation establishing a link between apelin and metabolic disorders (obesity, type 2 diabetes, etc.) which is the focus of the present review. Several studies, but not...

2011-01-01

256

Amelioration of phytotoxic effects of Cd on mung bean seedlings by gluconic acid secreting rhizobacterium Enterobacter asburiae PSI3 and implication of role of organic acid

British Library Electronic Table of Contents (United Kingdom)

Mung bean seedlings inoculated with Enterobacter asburiae PSI3, a gluconic acid-producing rhizosphere isolate, enhanced plant growth in the presence of phytotoxic levels of Cd2+ in gnotobiotic pot experiments as compared to the uninoculated Cd-treated plants. Addition of organic acids to Cd-stressed seedlings promoted root elongation. Hematoxylin competition assays showed that organic acids could displace Cd2+ from the Cd2+: hematoxylin complex in the same order of effectiveness as was found for restoration of root net elongation viz. oxalate?>?malate?>?succinate while gluconate was effective at higher concentrations. Root associated Cd2+, assessed by hematoxylin staining of roots was found to be reduced when roots were treated with organic acid. Cd stress increased antioxidant enzymes suc...

2008-01-01

257

A network security case study; The Los Alamos National Laboratory integrated computer network

Reactive biomolecular divergence in genetically altered yeast cells and isolated mitochondria as measured by biocavity laser spectroscopy : a rapid diagnostic method for studying cellular responses to stress and disease.

This paper reports on a study to validate the Graphical Network Representation (GRPHREP) model which is being conducted on the Los Alamos National Laboratory Integrated Computer Network (ICN). The GRPHREP model is a software system application based on graph theory and object-oriented programming methodologies. It codified the Department of Energy (DOE) Order 5637.1, which is concerned with classified computer secret policy, restrictions, and requirements. The Los Alamos ICN is required to control access to and support large-scale scientific and administrative computing. Thus, large-scale scientific and administrative computing. Thus we felt that this large, complex, and dynamic network would provide a good test for the graphical and functional capabilities of the model. Furthermore, the ICN is composed of multiple partitions that reflect the sensitivity and classification of the computation (data) and designate the required clearance level for the user.

1991-01-01

258

Studies on the mechanism of action of enterotoxin-induced fluid secretion in the gut

We report an analysis of four strains of baker's yeast (Saccharomyces cerevisiae) using biocavity laser spectroscopy. The four strains are grouped in two pairs (wild type and altered), in which one strain differs genetically at a single locus, affecting mitochondrial function. In one pair, the wild-type rho+ and a rho0 strain differ by complete removal of mitochondrial DNA (mtDNA). In the second pair, the wild-type rho+ and a rho- strain differ by knock-out of the nuclear gene encoding Cox4, an essential subunit of cytochrome c oxidase. The biocavity laser is used to measure the biophysical optic parameter Deltalambda, a laser wavelength shift relating to the optical density of cell or mitochondria that uniquely reflects its size and biomolecular composition. As such, Deltalambda is a powerful parameter that rapidly interrogates the biomolecular state of single cells and mitochondria. Wild-type cells and mitochondria produce Gaussian-like distributions ...

2006-12-01

259

Small-molecule screen identifies inhibitors of a human intestinal calcium-activated chloride channel.

The mechanism of action of Clostridium difficile enterotoxin A (CA), of Escherichia coli enterotoxin (STa) and of cholera toxin (CT), which are known to cause severe diarrhea, were studied in a preparation of ligated jejunal loops of anesthetized rats in vivo. The toxins were administered intraluminally. Pharmacological agents, which were tested for their potency to influence toxin-related effects, were administered subcutaneously. Net fluid transport was determined gravimetrically, prostaglandin (PG) E_2-output into the lumen, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) contents in the mucosa were measured by radioimmunoassay, serotonin-(5-HT)-output into the lumen was determined by high performance liquid chromatography. The histopathological effects of CA and CT were examined by light- and scanning electron microscopy. All three toxins caused net fluid secretion (FS). 5-HT_2-(ketanserin) and 5-HT_3-receptor antagonists ...

1992-01-01

260

Verification of lithium detector efficiency using DD neutron

Calcium-activated chloride channels (CaCCs) are widely expressed in mammalian tissues, including intestinal epithelia, where they facilitate fluid secretion. Potent, selective CaCC inhibitors have not been available. We established a high-throughput screen for identification of inhibitors of a human intestinal CaCC based on inhibition of ATP/carbachol-stimulated iodide influx in HT-29 cells after lentiviral infection with the yellow fluorescent halide-sensing protein YFP-H148Q/I152L. Screening of 50,000 diverse, drug-like compounds yielded six classes of putative CaCC inhibitors, two of which, 3-acyl-2-aminothiophenes and 5-aryl-2-aminothiazoles, inhibited by >95% iodide influx in HT-29 cells in response to multiple calcium-elevating agonists, including thapsigargin, without inhibition of calcium elevation, calcium-calmodulin kinase II activation, or cystic fibrosis transmembrane conductance regulator chloride channels. These compounds also inhibited ...

2007-12-14

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

261

Seasonal Variation in Heavy Metal Removal Efficiency and Application of Risk Assessment for Constructed Wetlands

The detection efficiency of a lithium glass detector was calculated using MCNP code, and the calculation was compared with the published results in Pulsed Sphere Plan. A lithium glass detector of our own was made, and its neutron efficiency was calculated. The calculated neutron efficiency was verified with both pulsed and steady DD neutrons. Characteristics of Neutron response of "6Li detector was discussed. (authors)

2005-08-01

262

Removal Efficiency of Organic Matter in Surface Flow Constructed Wetlands

Full Text Available

2010-11-01

263

Photochromes for Optical Memory

Full Text Available

264

International Science & Technology Center (ISTC)

Photochromes for New Generation of Highly Efficient Computers with Three Dimensional (3D) Optical Memory

265

Nutrients Removal Efficiency by Vegetation Density on Constructed Wetland from Small Watershed

Improving the efficiency of steam turbines; Verbesserung des Wirkungsgrades von Dampfturbinen

Full Text Available

2003-12-01

266

Improvement of the Radiation Efficiency for a CPW(Co-Planar Waveguide)-Fed ZOR(Zeroth-Order Resonant) Antenna

Short communication

1996-12-01

267

Efficiency enhancement of steam turbines: newly bladed; Wirkungsgradsteigerung von Dampfturbinen: Neu beschaufelt

Full Text Available

2011-01-01

268

Design of a Block-Based 2D Discrete Wavelet Transform Filter with 100% Hardware Efficiency

Short communication

1997-12-31

269

Design of H.264/AVC CABAC Encoder with an Efficient Storage Reduction of Syntax Elements

Full Text Available

2010-12-01

270

Comparison of Removal Efficiencies in Single and Duplex Ventilation Constructed Wetland Systems for Treating Domestic Sewage

Full Text Available

2010-04-01

271

A Study on the Application of the Mitigation System for Efficient Management of Coastal Wetlands in Korea -Enhancement of Legal Regime-

Full Text Available

2009-09-01

272

Comparative study on efficiency performance of listed coal mining companies in China and the US

Full Text Available

2003-12-01

273

British Library Electronic Table of Contents (United Kingdom)

Continually rising energy prices in global markets highlights a serious concern about the need to improve energy efficiency and the efficiency in energy sector in many countries. China, as one of the fastest growing countries in the world and the largest coal producer, has high coal consumption but a low recovery rate of coal utilization. Coal efficiency and the efficiency in coal industry have therefore attracted a great deal of attention from Chinese policy makers, coal firms and academics. This study attempts to compare the relative technical efficiency performance of listed coal mining companies in China and the US using CCR and BCC models in the advanced DEA linear programming. The results show that the level of relative efficiency in Chinese coal mining enterprises, regardless of tot...

2009-01-01

274

Studies on the biosorption of uranium by a thermotolerant, ethanol-producing strain of Kluyveromyces marxianus

Potential anti fungal agents. Synthesis and activity of 2-alkylthiopyridine-4-carbothioamides

The ability of residual biomass from the thermotolerant ethanol-producing yeast strain Kluyveromyces marxianus IMB3 to function as a biosorbent for uranium has been examined. It was found that the biomass had an observed maximum biosorption capacity of 120 mg U/g dry weight of biomass. The calculated value for the biosorption maximum, obtained by fitting the data to the Langmuir model was found to be 130 mg U/g dry weight biomass. Maximum biosorption capacities were examined at a number of temperatures and both the observed and calculated values obtained for those capacities increased with increasing temperature. Decreasing the pH of the biosorbate solution resulted in a decrease in uptake capacity. When biosorption reactions were carried out using sea-water as the diluent it was found that the maximum biosorption capacity of the biomass increased significantly. Using transmission electron microscopy, uranium crystals were shown to be concentrated on the outer ...

1997-06-01

275

NADP Regulates the Yeast GAL Induction System

A series of 2-alkylthiopyrine-4-carbothioamides were synthesized, and their anti-fungal potency was tested. The chemical structures were proved by infrared spectroscopy (IR) and {sup 1}H nuclear magnetic resonance ({sup 1}H-NMR) data and by elemental analysis. The minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) assessment were used for the estimation of potential activity in vitro. The study comprising 21 clinical isolates of fungi showed that two compounds exhibited fair inhibitory activity against some yeasts and dermatophytes. Selective fungistatic activity against non-dermatophytes (MIC = 3.12-25.0 {mu}g/mL) was found also in another compound. None of the above compounds showed inhibitory activity against non-dermatophyte filamentous fungi. Microbiological activity of 2-alkylthiopyridine-4-carbothioamides appears to be mainly related to hydrophobicity of alkyl in position 2. (authors). 10 refs., 8 tabs.

1996-05-01

276

Molecular studies of the uncoupling protein

Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UASGAL); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.

2008-01-01

277

Evaluation of heavy-metal ion toxicity in fish cells using a combined stress protein and cytotoxicity assay

The uncoupling protein (UCP) is a proton/anion transporter found in the inner mitochondrial membrane of brown adipocyte. Although UCP has nor been detected in mitochondria from any other tissue, it shares structural and catalytic properties with several other mitochondrial carrier proteins. Although UCP was discovered only recently it is one of the most extensively studied mitochondrial carrier proteins.More recently, the mouse, rat, and human genes encoding for UCP have been isolated and sequenced. The availability of these various tools has led to several significant observations. UCP gene expression is strongly controlled at the level of transcription by signals that are activated after the stimulation of brown adipocytes by norepinephrine. The comparison of UCP gene with the genes encoding the adenine nucleotide translocator revealed the existence of structural and evolutionary homologies. Moreover, in humans the UCP gene and one form of adenine nucleotide translocator gene are ...

1991-06-01

278

Effects of paraquat on Escherichia coli: Differences between B and K-12 strains

All organisms, from bacteria and yeast to humans, respond to physical and chemical stressors by increasing the synthesis of a small group of cellular stress proteins.'' The authors have developed a simple in vitro system for quickly screening environmentally relevant stressors to detect stress-induced proteins that are good candidates for biomarkers. Polyacrylamide gel electrophoresis was used to detect stressor-induced, concentration-dependent changes in cellular stress protein levels in two fish cell culture systems, whereas simultaneous in vitro neutral red uptake cytotoxicity assays measured the stressors effect on cellular physiology. There was a direct concentration-dependent relationship between sublethal cytotoxic effects and the increases in stress protein levels. Increases of 50 to 200% were detected in stress proteins from desert topminnow, Poeciliopsis lucida, hepatoma-derived cell cultures exposed to cadmium or copper. Three proteins ...

1994-08-01

279

Construction of a human MluI YAC library

Escherichia coli B and K-12 are equally susceptible to the bacteriostatic effects of aerobic paraquat, but they differed strikingly when the lethality of paraquat was evaluated. E. coli B suffered an apparent loss of viability when briefly exposed to paraquat, whereas E. coli K-12 did not. This difference depended on the ability of the B-strain, but not the K-12 strain, to retain internalized paraquat; the B strain was killed on aerobic tryptic soy-yeast extract plates during the incubation which preceded the counting of colonies. This difference in retention of paraquat between strains was demonstrated by delayed loss of viability, by growth inhibition, and by cyanide-resistant respiration after brief exposure to paraquat, washing, and testing in fresh medium. This difference was also shown by using ({sup 14}C)paraquat. This previously unrecognized difference between E. coli B and K-12 has been the cause of apparently contradictory reports and should lead to some ...

1990-02-01

280

Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22

The authors describe a cloning strategy for the construction of a human genomic library in yeast artificial chromosomes (YACs) based on complete digestion of high-molecular-weight DNA with the infrequently cutting restriction enzyme MluI. Cloning of MluI fragments in the vector pYAC-RC and one subsequent size fractionation by preparative pulsed-field gel electrophoresis yielded a library with average insert sizes of 600 kb. Ninety-seven percent of the colonies were recombinant. An additional size fractionation of MluI fragments prior to ligation had no significant influence on the size of the YACs. The library currently consists of 5000 clones, which is the equivalent of one human genome. Nineteen percent of the YACs were larger than 1.2 Mb. Since smaller MluI fragments are lost during sizing, they also performed cloning without size fractionation. Only 20% of the colonies were recombinant, probably due to unligated vector fragments that were present during the ...

1994-05-01

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281

Biosorption of Ni (II) by Schizosaccharomyces pombe: kinetic and thermodynamic studies

Two overlapping cDNA clones encoding human DNA topoisomerase II were identified by two independent methods. In one, a human cDNA library in phage {lambda} was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and Drosophila DNA topoisomerase II; in the other, a different human cDNA library in a {lambda}gt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific to human DNA topoisomerase II. The entire coding sequences of the human DNA topoisomerase II gene were determined from these and several additional clones, identified through the use of the cloned human TOP2 gene sequences as probes. Hybridization between the cloned sequences and mRNA and genomic DNA indicates that the human enzyme is encoded by a single-copy gene. The location of the gene was mapped to chromosome 17q21-22 by in situ hybridization of a cloned fragment to ...

1988-10-01

282

British Library Electronic Table of Contents (United Kingdom)

The potential of the dried yeast, wild-type Schizosaccharomyces pombe, to remove Ni(II) ion was investigated in batch mode under varying experimental conditions including pH, temperature, initial metal ion concentration and biosorbent dose. Optimum pH for biosorption was determined as 5.0. The highest equilibrium uptake of Ni(II) on S. pombe, q e, was obtained at 25??C as 33.8?mg?g?1. It decreased with increasing temperature within a range of 25?50??C denoting an exothermic behaviour. Increasing initial Ni(II) concentration up to 400?mg?L?1 also elevated equilibrium uptake. No more adsorption took place beyond 400?mg?L?1. Equilibrium data fitted better to Langmuir model rather than Freundlich model. Sips, Redlich?Peterson, and Kahn isotherm equations modelled the investigated system with a...

2011-01-01

283

A procedure for batch separation of sup 14 C-hexose from sup 14 C-sucrose

Theoretical study on device efficiency of pulsed liquid jet pump

This presentation describes a method for separating {sup 14}C-hexose from {sup 14}C-sucrose in extracts of plant tissue. Portions of ethanol extracts are treated with activated charcoal in microcentrifuge tubes. Aliquots are removed, ethanol evaporated and replaced with reaction mixture that phosphorylates hexose (HEPPS, K{sub 2}HPO{sub 4}, Mg(C{sub 2}H{sub 3}O{sub 2}){sub 2}, ovalbumen, Na{sub 2}ATP, yeast hexokinase). After a time course, the hexokinase reaction is stopped (slowed considerably) to minimize effects of contamination enzyme activities. The stopping agent used is lyxose, a nonphosphorylable analogue of glucose. The strong anionic charge of phosphate introduced through the hexokinase action results in binding (> 95%) of hexose-phosphate to anion-exchange resin. Sucrose remains unbound (> 95%) in solution. This batch ion-exchange is performed in microcentrifuge tubes to allow many samples to be processed simultaneously. Recovery of radiolabel in ...

1991-05-01

284

Improvement of the efficiency of a bare solar collector by means of turbulence promoters

The influence of the main factors on device efficiency of pulsed liquid jet pump with gas-liquid piston is analysed, the theoretical equation and its time-averaged solution of pulsed liquid jet pump device efficiency are derived. The theoretical and experimental results show that the efficiency of transmission of energy and mass to use pulsed jet is greatly raised, compared with steady jet, in the same device of liquid jet pump. The calculating results of time-averaged efficiency of pulsed liquid jet pump are approximately in agreement with the experimental results in our and foreign countries

2001-03-01

285

An assessment of the building sector efficiency resource for the Town of Handlova

A steady-state mathematical model was developed for a bare collector. Results obtained by previous authors for heat transfer and friction in rectangular ducts with periodic disturbances were employed to evaluate the effect of such disturbances on bare collector efficiency and on its pressure drop. An effective efficiency, which takes into account extra heat obtained and additional mechanical (electric) energy consumption for moving air when heat-transfer augmentation devices are employed, was defined. Simulation results are given for a bare collector, with and without perturbations, showing efficiency and effective efficiency dependence on collector dimensions, solar radiation intensity, disturbance diameter and pitch for collector performance optimization. (author).

1990-01-01

286

[Natriuretic peptides--relevance in intensive care].

Purpose of this assessment for this town in the Slovak Republic is to develop information on the building sector energy efficiency and improvement potential for use by town management to support decisions on providing energy services to Handlova citizens. Objectives were to characterize baseline space and water heat energy use and efficiency improvement potential in the residential and nonresidential building sectors. Intention was to identify major areas of efficiency improvement potential and cost-effectiveness. Four levels of energy and fuel prices were used. A significant efficiency resource exists in the space and water heating end uses in these building sectors; it amounts to 42% of the total building sector space and water heat energy consumption.

1995-10-01

287

Transforming growth factor-b induces nerve growth factor expression in pancreatic stellate cells by activation of the ALK-5 pathway

The family of natriuretic peptides consists of the atria natriuretic peptide (ANP), the cerebral natriuretic peptide (BNP), the type C natriuretic peptide (CNP) and the peptide isolated from the dendroaspis snakes' poison (DNP), whose presence in humans has not been confirmed. The physiological function of ANP is in the control of arterial blood pressure by regulation of systemic vascular resistance of blood vessels. BNP is produced as one of the factors in the acute response to inflammatory tissue damage, mainly in coronary vessels. Increased serum concentrations of natriuretic peptides have been found in stress situations, such as trauma or major surgery, systemic hypotension, and in intrinsic myocardial dysfunction. High concentrations of natriuretic peptides were observed in severe sepsis, septic shock and in multiple organ failure, probably due to increased secretion by mediators of the inflammatory process.The highest concentrations of ANP and BNP were found ...

288

British Library Electronic Table of Contents (United Kingdom)

Nerve growth factor (NGF), a survival factor for neurons enforces pain by sensitizing nociceptors. Also in the pancreas, NGF was associated with pain and it can stimulate the proliferation of pancreatic cancer cells. Hepatic stellate cells (HSC) respond to NGF with apoptosis. Transforming growth factor (TGF)-b, one of the strongest pro-fibrogenic activators of pancreatic stellate cells (PSC) induced NGF and its two receptors in an immortalized human cell line (ihPSC) and primary rat PSC (prPSC) as determined by RT-PCR, western blot, and immunofluorescence. In contrast to HSC, PSC expressed both NGF receptors, although p75NTR expression was weak in prPSC. In contrast to ihPSC TGF-b activated both Smad signaling cascades in prPSC. NGF secretion was diminished by the activin-like kinase (ALK)...

2009-01-01

289

The frontal weapon of the termite Armitermes euamignathus Silvestri (Isoptera, Termitidae, Nasutitermitinae)

Scientific Electronic Library Online (English)

Abstract in english Frontal weapon is the term used to designate the apparatus consisting of frontal gland and associated structures that participate in the chemical defense of termite soldiers. The ultra structure of the frontal gland and the scanning microscopy of the soldier head was investigated in the termite Amiitermes euamignathus Silvestri, 1901.Campaniform sensilla was not observed but there were 80 to100 sensory bristles around the frontal pore. The glandular epithelium shows only (more) class 1 cells according 10 the classification of NOIROT & QUENNEDEY (1974, 1991). The glandular cells are characterized by apical microvilli, a basal labyrinth and a large quantity of smooth endoplasmic reticulum which forms dense zones throughout the cytoplasm. The secretion is concentrated mainly in the basal pole of the cell and consists of large lipid droplets. The secretory epithelium is covered by a thick apical cuticle composed of a thin outer epicuticle, a ...

2001-06-01

290

Studies on localization of calcitonin gene-related peptide (CGRP) in the thyroid-parathyroid complex.

Steganography using wavelet compressed images

Calcitonin gene-related peptide (CGRP) was localized by an immunocytochemical technique in the thyroid-parathyroid complexes of rat, guinea pig, rabbit, and in normal human thyroids and parathyroids. Human medullary carcinomas and parathyroid adenomas were also studied. In man and all animal species examined CGRP was present in the parafollicular cell, however, in guinea pigs only in small amounts. Except in rabbits, presence of CGRP was demonstrated in nerves of the thyroid and parathyroid capsule as well as in the nerve fibers of the capsular blood vessels. In the thyroid of guinea pigs CGRP was also noted in nerve fibers and in blood vessel walls between follicles. CGRP was also present in the parathyroid glands of rat and man, in nerve fibers localized between parathyroid cells. In rabbit the parafollicular cells between parathyroid cells also expressed CGRP immunoreactivity. No CGRP was noted in the parathyroids of the guinea pig. The proximity of parathyroid cells and CGRP ...

1987-01-01

291

Sertoli cells in culture secrete paracrine factor(s) that inhibit peritubular myoid cell proliferation: identification of heparinoids as likely candidates

Internet bandwidth is in high demand, and one way that web sites lower the amount of bandwidth they use is by compressing their site's images. This lowers the amount of bandwidth used, and makes the site load much faster. There are of course many other useful applications for compressed images. Bit Plane Complexity Segmentation (BPCS) digital picture steganography is a technique to hide data inside an image file. BPCS achieves high embedding rates with low distortion based on the theory that noise-like regions in a bit-plane can be replaced with noise-like secret data without discernible loss in image quality. This is possible because the human eye, while very good at distinguishing anomalies in areas of homogenous texture, is bad at distinguishing anomalies in visually complex areas. However, BPCS is not a robust embedding scheme, and any lossy compression usually destroys the data. Wavelet image compression using the Discreet Wavelet Transform (DWT) is the basis ...

2001-08-01

292

Salivary cortisol assessment in the evaluation of hypothalamic-pituitary-adrenal function

Conditioned medium from Sertoli cells, prepared from testes of 20-day-old rats, contains component(s) that inhibit the incorporation of (3H)-thymidine into DNA of peritubular myoid cells (PMC) and inhibit the proliferation of PMC. These components are trypsin-resistant, heat-stable compounds having a molecular weight less than 30,000. The active inhibitory components in Sertoli cell conditioned medium are inactivated by treatment with heparinase, but not by treatment with hyaluronidase or chondroitin sulfate lyases. Addition of heparin or heparan sulfate results in inhibition of DNA synthesis by PMC in a dose-dependent manner, whereas other glycosaminoglycans (GAGs) examined (hyaluronic acid, keratan sulfate, and chondroitin sulfate) have no detectable effects. Heparin and heparan sulfate are unique among GAGs tested in inhibiting the characteristic multilayer growth pattern of PMC following the attainment of confluence in serum-rich medium. On the basis of these and other data ...

1991-06-01

293

Pharmacokinetics of multiple doses of valsartan in patients with heart failure.

A simple, short and sensitive direct radio-immunoassay technique for the determination of salivary cortisol concentration was employed to assess saliva as a medium for evaluating cortisol response during endocrine testing in 9 controls and 40 patients. Results in controls suggested that an adequate salivary cortisol response to insulin hypoglycaemia was an increase of 150% above the basal value with a minimum peak of 15 nmol/l. Thirty-three patients were classified as being either good or poor responders to insulin hypoglycaemia on the basis of criteria for plasma cortisol levels. When the defined salivary cortisol response was used for assessment, all 33 patients were correctly categorized into the same response groups. The salivary cortisol response to intramuscular tetracosactin in 3 patients and an intravenous dexamethasone infusion in 4 patients confirmed the value of saliva as an assay medium. These studies show that the salivary cortisol response parallels that of total plasma ...

1984-05-01

294

Netware-Specific Network Security.

Angiotensin II has adverse actions in heart failure including vasoconstriction, aldosterone secretion, and activation of the sympathetic nervous system. Valsartan, a potent specific angiotensin II type 1 receptor blocker, may produce beneficial effects in heart failure. The purpose of this study was to evaluate the steady-state pharmacokinetics of valsartan 40, 80, and 160 mg each given every 12 h for 7 days in heart failure patients. Eighteen patients with chronic stable heart failure and left ventricular ejection fractions power model, showed that doubling the dose increased the AUC and Cmax 1.8 times. The pharmacokinetics of valsartan are predictable in heart failure patients within the dose range of 40-160 mg BID. Age did not appear to have influenced the valsartan clearance in heart failure patients. The pharmacokinetic values were higher in heart failure patients than in healthy volunteers. All doses were generally safe and well tolerated.(max) (max) ...

2002-11-01

295

Frame Selected Approach for Hiding Data within MPEG Video Using Bit Plane Complexity Segmentation

This paper focuses on practical and cost-effective NetWare-specific approaches to information systems and computer security. A series of real world experiences is presented that illustrate fundamental information systems and security concepts. A NetWare network is a client-server network which uses a file server to share files while client workstations access the file server via some network typology (usually ethernet or token ring). Passwords are used to authenticate users; thus, password protection is a cornerstone of network security. Passwords can be encrypted, but this feature can be disabled. The bindery, which stores information about users, groups, and printers, is critical to the network and must be backed up. Trade secrets can be hidden from users by restructuring menus. Login scripts should be protected and not used for security purposes. Guest account access should be restricted or removed. Finally, the "security.exe" program can be used to find ...

1994-12-01

296

Enzyme-linked immunosorbent assays for alpha-synuclein with species and multimeric state specificities.

Bit Plane Complexity Segmentation (BPCS) digital picture steganography is a technique to hide data inside an image file. BPCS achieves high embedding rates with low distortion based on the theory that noise-like regions in an image's bit-planes can be replaced with noise-like secret data without significant loss in image quality. . In this framework we will propose a collaborate approach for select frame for Hiding Data within MPEG Video Using Bit Plane Complexity Segmentation. This approach will invent high secure data hidden using select frame form MPEG Video and furthermore we will assign the well-built of the approach; during this review the author will answer the question why they used select frame steganography. In additional to the security issues we will use the digital video as a cover to the data hidden. The reason behind opt the video cover in this approach is the huge amount of single frames image per sec which in turn overcome the problem of the data ...

2009-01-01

297

Effect of clindamycin treatment on vaginal inflammatory markers in pregnant women with bacterial vaginosis and a positive fetal fibronectin test

Abnormal intracellular deposition of aggregated ?-synuclein is the characteristic feature of a number of neurological disorders, including Parkinson's disease (PD). Although ?-synuclein is typically known as a cytosolic protein, a small amount is secreted by exocytosis in both monomeric and aggregated forms. The extracellular forms of ?-synuclein in human body fluids, such as cerebrospinal fluid (CSF) and blood plasma, might be a diagnostic target for PD and related diseases. Here, we characterized a new set of monoclonal antibodies against ?-synuclein, and using different combinations of antibodies, we established ELISA systems to specifically detect human ?-synuclein, mouse and human ?-synuclein together, and multimeric forms of ?-synuclein in biological samples. By employing the Tyramide signal amplification method, the sensitivity of the assay was significantly improved to detect a concentration as low as ?12.5 pg/ml. These assays might be useful tools for ...

2011-05-31

298

British Library Electronic Table of Contents (United Kingdom)

Objective To compare the levels of interleukin (IL)-1?, IL-6, and matrix metalloproteinase (MMP)-8 in the vaginal secretions of pregnant women with a positive fetal fibronectin (fFN) test result with or without asymptomatic bacterial vaginosis (BV) before and after treatment with oral clindamycin. Methods A prospective cohort study was conducted among 43 pregnant women with a positive fFN test result. All patients were treated with clindamycin, and the pre- and post-treatment levels of IL-1?, IL-6, and MMP-8 were compared. Results Before treatment, levels of IL-1? and MMP-8 were significantly higher in women with BV compared with women without BV (P<0.05). Vaginal levels of IL-1? and IL-6, but not MMP-8, decreased after treatment in pregnant women with BV. Conclusions The inability of clin...

2009-01-01

299

Constituents from Maytenus ilicifolia leaves and bioguided fractionation for gastroprotective activity

Celastrol regulates innate immunity response via NF-@kB and Hsp70 in human retinal pigment epithelial cells

Maytenus ilicifolia Mart. ex Reissek is traditionally used in Brazil for treatment of gastric ulcers. Here we report the phytochemical investigation of an ethanol extract of M. ilicifolia leaves (EEMIL) aiming at the isolation of constituents which were used as chemical markers to monitor an activity-guided fractionation of a lyophilized aqueous extract of M. ilicifolia leaves (LAEMIL). From EEMIL, four flavonoids were isolated, namely the tri-flavonoid glycosides mauritianin (1), trifolin, (2) hyperin (4), and epi-catechin (5). Fractionation of LAEMIL led to 5 fractions which afforded the tetra-glycoside kaempferol derivative (3), and galactitol (6). LAEMIL and its fractions were evaluated in rats for their effects on gastric secretion volume and pH. HPLC (High Performance Liquid Chromatography) analysis revealed that only fractions containing the tri- and tetra-flavonoid glycosides 1 and 3 caused significant increase of gastric volume and pH, thus indicating that ...

300

British Library Electronic Table of Contents (United Kingdom)

Elevated nuclear factor kappa B (NF-@kB) activity and interleukin-6 (IL-6) secretion participates in the pathology of several age and inflammatory-related diseases, including age-related macular degeneration (AMD), in which retinal pigment epithelial cells are the key target. Recent findings reveal that heat shock protein 70 (Hsp70) may affect regulation of NF-@kB. In the current study, effects of Hsp70 expression on NF-@kB RelA/p65 activity were evaluated in human retinal pigment epithelial cells (ARPE-19) by using celastrol, a novel anti-inflammatory compound. Anti-inflammatory properties of celastrol were determined by measuring expression levels of IL-6 and endogenous NF-@kB levels during lipopolysaccharide (LPS) exposure by using enzyme-linked immunosorbent assays (ELISA). Cell viabil...

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

301

Comparative study on efficiency performance of listed coal mining companies in China and the US

Comparative study on efficiency performance of listed coal mining companies in China and the US

Continually rising energy prices in global markets highlights a serious concern about the need to improve energy efficiency and the efficiency in energy sector in many countries. China, as one of the fastest growing countries in the world and the largest coal producer, has high coal consumption but a low recovery rate of coal utilization. Coal efficiency and the efficiency in coal industry have therefore attracted a great deal of attention from Chinese policy makers, coal firms and academics. This study attempts to compare the relative technical efficiency performance of listed coal mining companies in China and the US using CCR and BCC models in the advanced DEA linear programming. The results show that the level of relative efficiency in Chinese coal mining enterprises, regardless of total technical efficiency or decomposed pure technical ...

2009-12-15

302

Comparative study on efficiency performance of listed coal mining companies in China and the US

Continually rising energy prices in global markets highlights a serious concern about the need to improve energy efficiency and the efficiency in the energy sector in many countries. China, as one of the fastest growing countries in the world and the largest coal producer, has high coal consumption but a low recovery rate of coal utilization. Coal efficiency and the efficiency in coal industry have therefore attracted a great deal of attention from Chinese policy makers, coal companies and academics. This study attempts to compare the relative technical efficiency performance of listed coal mining companies in China and the US using CCR and BCC models in advanced data envelopment analysis (DEA) linear programming. The results show that the level of relative efficiency in Chinese coal mining enterprises, regardless of total technical ...

2009-12-15

303

Turbines for the turn of the century

Continually rising energy prices in global markets highlights a serious concern about the need to improve energy efficiency and the efficiency in energy sector in many countries. China, as one of the fastest growing countries in the world and the largest coal producer, has high coal consumption but a low recovery rate of coal utilization. Coal efficiency and the efficiency in coal industry have therefore attracted a great deal of attention from Chinese policy makers, coal firms and academics. This study attempts to compare the relative technical efficiency performance of listed coal mining companies in China and the US using CCR and BCC models in the advanced DEA linear programming. The results show that the level of relative efficiency in Chinese coal mining enterprises, regardless of total technical efficiency or decomposed pure technical ...

2009-12-15

304

Spatial distribution of IGC-30 Ge-detector's efficiency

This article describes how a natural gas-fired combined cycle with increased firing temperature, reduced cooling air usage, improved component efficiencies, and cycle enhancements could achieve a lower heating value plant efficiency of greater than 60%. Today's gas turbine systems feature high fuel-to-electricity efficiencies. Efficiencies, on a lower-heating-value (LHV) basis, for large natural gas-fired combined-cycle systems for the utility market have been demonstrated at 54%. Even though manufacturers will make improvements in the 1990s, efficiency levels will reach a plateau. Cycle innovations and gas turbine design changes will achieve LHV efficiencies in the 60% range for natural gas-fired utility machines.

1994-06-01

305

The effect of physician practice organization on efficient utilization of hospital resources.

The dependence of the efficiency of a coaxial IGC-30 Ge detector on the direction of gamma quanta entry, their energy and the distance from the source is examined. A set of point sources QCR-2 (Amersham, UK) has been used which is arranged consecutively in two perpendicular planes passing through the detector axis, at a distance of 0.1 - 1.3 m (pace 0.1) from its geometric centre for 16 different angles in every plane. Dependence curves of the efficiency on the angle of gamma-quanta hits are obtained for energies 60, 81, 122, 356, 662, 834, 1173 and 1332 keV. Changes in efficiency connected with spatial dividing ability are found out. Using the least squares' method the expected spatial distribution of efficiency is examined with a level of authenticity P > 0.9. The possible causes for the efficiency change and its possible effect on the measurements' precision are discussed. ...

1993-12-02

306

The between and within day variation in gross efficiency

OBJECTIVE. This study examines variations in the efficient use of hospital resources across individual physicians. DATA SOURCES AND SETTING. The study is conducted over a two-year period (1989-1990)...Full Text Available

1994-12-01

307

Round Trip Energy Efficiency of NASA Glenn Regenerative Fuel ...

Before the influence of divergent factors on gross efficiency (GE) [the ratio of mechanical power output (PO) to metabolic power input (PI)] can be assessed, the variation in GE between days, i.e. the...Full Text Available

2010-08-01

308

Renewable energy and the ODA energy efficient strategy

Analex Corporation, Brook Park, Ohio. Round Trip Energy Efficiency of NASA Glenn. Regenerative Fuel Cell System. NASA/TM2006-214054. January 2006 ...

309

PEG-conjugated PAMAM Dendrimers Mediate Efficient Intramuscular Gene Expression

The energy strategy of the UK Overseas Development Administration is outlined with specific emphasis on energy efficiency and renewable energies. Based on 29 case studies concerning projects and programmes in both developed and developing countries, critical success factors for renewable energy projects are identified. UK)

1995-12-31

310

Method of restoring degraded solar cells

Generations 5 and 6 (G5 and G6) poly(amidoamine) (PAMAM) dendrimers have been shown to be highly efficient nonviral carriers in in vitro gene delivery. However, their high toxicity...Full Text Available

311

Highly efficient concentration of lenti- and retroviral vector preparations by membrane adsorbers and ultrafiltration

Amorphous silicon solar cells have been shown to have efficiencies which degrade as a result of long exposure to light. Annealing such cells in air at a temperature of about 200.degree. C. for at least 30 minutes restores their efficiency.

1983-01-01

312

Flue gas desulfurization: scrubber types and selection criteria

BackgroundLentiviral vectors (LVs) can efficiently transduce a broad spectrum of cells and tissues, including dividing and non-dividing cells. So far the most widely used method...Full Text Available

313

Flashlamp radiation recycling for enhanced pumping efficiency and reduced thermal load

Economic and performance criteria that should be used in the selection of a scrubber system are presented. Selection should be based on the type of emissions put out by a plant and the removal efficiency required by pollution control standards. Costs and efficiencies for 10 different scrubber systems are reported. (10 diagrams)

1980-05-01

314

Environmental effects and energy efficiency in building design: A green building approach. Part 1, energy efficiency techniques. Research report

A method for recycling laser flashlamp radiation in selected wavelength ranges to decrease thermal loading of the solid state laser matrix while substantially maintaining the pumping efficiency of the flashlamp.

1989-01-01

315

Environmental Effects and Energy Efficiency in Building Design: A Green Building Approach. Part 1, Energy Efficiency Techniques.

;Contents: Energy Use; Building Fabric Performance; Ventilation and Infiltration; Passive Solar Design; Heating Systems and Controls; Hot and Cold Water Provision; and Lighting and Electrical Appliances.

1993-01-01

316

metadata, images and captions. - The Gateway to Astronaut ...

Contents: Energy Use; Building Fabric Performance; Ventilation and Infiltration; Passive Solar Design; Heating Systems and Controls; Hot and Cold Water Provision; and Lighting and Electrical Appliances.

1993-01-01

317

Treatment Efficiency of Pollutants in Constructed Wetlands under Different Hydroponic Wastewater Injection Methods and Characteristic of Filter Media

... the Zaire is not an efficient transportation link because of many rapids and falls to negotiate between Kinshasa and the port city of Matadi. ...

318

The Mission, Organization, and Functions of US Army Rear ...

Full Text Available

2010-06-01

319

Subsolutions of an Isaacs Equation and Efficient Schemes for ...

... counseling, some training, and assure the general health and welfare of the command. ... publications, promotions, OERs/NCOER's (Officer Efficiency ...

1992-06-07

320

Smoke Compositions Based on Phosphorus

... analyze importance sampling schemes for stochastic networks ... an interesting mixed open/closed queueing network ... with a suitable terminal condition ...

2005-08-08

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321

Review of the application of molecular beam epitaxy for high efficiency solar cell research

... Both magnesium and aluminium phosphides are therefore potentially more efficient than either calcium or sodium phosphide. ...

1958-03-01

322

Radiobiological evaluation of efficiency-risk relationships of the pelviogram in medical check-up of sports

In the last two years, rapid progress has been made in the energy conversion efficiencies of GaAs solar cells fabricated from molecular beam epitaxy (MBE) material. The efficiencies of cells fabricated from MBE material are now comparable with those fabricated from metal-organic chemical vapor deposition material, even for cells of dimension 2 cmx4 cm. This paper reviews the progress in MBE cell efficiencies. Also discussed is the role oval defects play in GaAs diode and solar cell performance. (orig.).

1991-05-01

323

Measurement of low-energy antiproton detection efficiency in BESS below 1 GeV

... body body areas diagnostic techniques hazards health hazards mammals

1984-01-16

324

An accelerator experiment was performed using a low-energy antiproton beam to measure antiproton detection efficiency of BESS, a balloon-borne spectrometer with a superconducting solenoid. Measured efficiencies showed good agreement with calculated ones derived from the BESS Monte Carlo simulation based on GEANT/GHEISHA. With detailed verification of the BESS simulation, the relative systematic error of detection efficiency derived from the BESS simulation has been determined to be {+-}5%, compared with the previous estimation of {+-}15% which was the dominant uncertainty for measurements of cosmic-ray antiproton flux.

2002-08-21

325

JW" rV-- - NASA

Is the US Army a Learning Organization?

roller bearing strength suffi- cient to support efficient, rolling friction movement, even in the presence of large, resisting forces. ...

326

High-efficiency resonant rf spin rotator with broad phase space acceptance for pulsed polarized cold neutron beams

... outside observers, and updated counseling requirements for the Army's OER, non-commissioned officer efficiency report (NCOER), and civilian ...

2007-03-08

327

High efficiency USC power plant - present status and future potential

No abstract prepared.

2008-01-01

328

Evaluation of Pollutant Removal Efficiency in Environmentally Friendly Full-scale Constructed Wetlands for Treating Domestic Sewage during Long-term Monitoring

Increasing demand for energy production with low impact on the environment and minimised fuel consumption can be met with high efficient coal fired power plants with advanced steam parameters. An important key to this improvement is the development of high temperature materials with optimised mechanical strength. Based on the results of more than ten years of development a coal fired power plant with an efficiency above 50 % can now be realised. Future developments focus on materials which enable an efficiency of 52-55 %. (orig.) 25 refs.

1998-12-31

329

Evaluating the Efficiency of Asynchronous Systems with FASE

Full Text Available

2009-06-01

330

In this paper, we present FASE (Faster Asynchronous Systems Evaluation), a tool for evaluating the worst-case efficiency of asynchronous systems. The tool is based on some well-established results in the setting of a timed process algebra (PAFAS: a Process Algebra for Faster Asynchronous Systems). To show the applicability of FASE to concrete meaningful examples, we consider three implementations of a bounded buffer and use FASE to automatically evaluate their worst-case efficiency. We finally contrast our results with previous ones where the efficiency of the same implementations has already been considered.

2011-01-01

331

Connections

CARBON DIOXIDE REDUCTION SYSTEM

... many intriguing new possibilities in the materials sciences. Or consider solitons, a mathematical ... point out that advanced instrumentation, new materials, novel production methods, and efficient ...

332

Army Symposium: 'Leadership Challenges of the 21st Century ...

... be easily replaceable, and its compartment or container ... in a simple, efficient manner for storage or disposal. ... and enters the reactor at approximatel ...

1963-01-01

333

Absolute calibration of the antiproton detection efficiency for BESS below 1 GeV

... or the Noncommissioned Officer Efficiency Report (NCOER) for promotion ... Providing effective coaching, delegating, counseling, and role modeling ...

1996-05-01

334

A Secure and Efficient Communications Architecture for ...

An accelerator beam experiment was performed using a low-energy antiproton beam to measure antiproton detection efficiency of the BESS detector. Measured and calculated efficiencies derived from the BESS Monte Carlo simulation based on GRANT/GHEISHA showed good agreement. With detailed verification of the BESS simulation, the relative systematic error of detection efficiency derived from the BESS simulation has been determined to be {+-}5%, compared with the previous estimation of {+-}15% which was the dominant uncertainty for measurements of cosmic-ray antiproton flux. (author)

2001-12-01

335

A Procedure for the separation of sub-microgram quantities of lead and bismuth by vacuum evaporation

... Milstar Military Strategic and Tactical Relay Satellite ... (EHF) system, composed of the Military Strategic and Tactical Relay Satellite (Milstar) ...

2008-06-19

336

A NEW FORM OF SOLID STATE SOLAR GENERATOR

... bismuth chlorides bismuth 207 cadmium chlorides efficiency evaporation high

337

A Medical Integration Framework based on XML for efficient exchange and sharing of Electronic Health Record using HL7

... nent to the design and construction of metal-semiconductor solar cells, in that both the photovoltage and the efficiency of metal-semiconductor cells ...

1962-01-01

338

The market efficiency in the stock markets

Full Text Available

2002-10-01

339

Simplification of the scheme - Department of Energy and Climate Change

We study the temporal evolution of the market efficiency in the stock markets using the complexity, entropy density, standard deviation, autocorrelation function, and probability distribution of the log return for Standard and Poor's 500 (S&P 500), Nikkei stock average index, and Korean composition stock price index (KOSPI). Based on the microscopic spin model, we also find that these statistical quantities in stock markets depend on the market efficiency.

2007-01-01

340

Pulsed plasma processing of effluent pollutants and toxic chemicals

...Simplification of the scheme - Department of Energy and Climate Change simplification, crc energy efficiency scheme Simplification of the scheme Simplification of the CRC Energy ...Efficiency Scheme The Government is considering the simplification of the CRC in the light of experience since the scheme began in April 2010. ...will take into account feedback from stakeholders including: the effectiveness of the CRC framework for driving energy efficiency in large private and public sector ... The perceived complexity of the CRC scheme and hence the administrative burden on: - those organisations that are subject to ...

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

341

Environmental effects and energy efficiency in building design - a green building approach. Pt. 3. Design case studies

The author is exploring the efficiency of pulsed plasma processing in the removal of nitrogen dioxide, nitrogen oxide, and other pollutants. This process uses an electrical discharge to create chemical radicals from air molecules. These radicals can react with pollutants and form harmless compounds. Additives such as hydrocarbons are also used to improve the efficiency of the removal. The efficient removal of nitrogen dioxide has required the presence of dilute aqueous solution of ammonia.

1994-05-01

342

Environmental effects and energy efficiency in building design - a green building approach. Pt. 1. Energy efficiency techniques

This is the third of a series of research reports which builds on the previous contents which dealt with energy efficiency techniques and minimizing the environmental effects of building materials. It presents some specifications for house types and gives built examples of energy efficient homes in Europe. (UK)

1993-12-31

343

Efficient operation of equipment at the mine ''Tsentral'naya'' and ways to improve it

A research report describes the energy efficiency techniques to be employed in designing a building which is ``green``. Topics covered include building fabric performance, ventilation and infiltration, passive solar design, heating systems and controls, hot and cold water provision, lighting and electrical appliances. (UK)

1993-12-31

344

Determination of efficiency curves of germanium detectors using natural radioactive materials

Results are presented from analyzing indicators for efficient operation of the fleet of production equipment under conditions of the ''Tsentral'naya'' mine of the production association ''Krasnoarmeyskugol'.'' Measures are presented which make it possible to increase the operating efficiency with minimum adjusted outlays.

1980-01-01

345

A study of primary sulfate emissions from a coal-fired boiler with FGD. [Flue gas desulfurization

Efficiency calibration curves for different densities and geometries are necessary for activity determination of various environmental samples. Commercially available standards or natural radioactive materials are used for calibration. Potassium compounds are especially suitable, since they can be mixed with unknown samples. It is possible to determine efficiency curve and unknown activity of sample knowing "4"0 K activity. (author).

346

Global residential appliance standards

An extended series of measurements at a conventional coal-fired boiler equipped with a wet-limestone flue gas desulfurization unit has shown that sulfate and sulfur dioxide removal efficiencies are dissimilar. The average SO2 scrubbing efficiency was 76% and was observed to decrease over a five-day operating cycle. Total sulfate scrubbing efficiency, averaging about 29%, was invariant throughout the operating cycle. (1 diagram, 3 tables)

1979-09-01

347

Actual and future strategies in interdisciplinary treatment of medulloblastomas, supratentorial PNET and intracranial germ cell tumors in childhood; Aktuelle und zukuenftige Strategien in der interdisziplinaeren Therapie von Medulloblastomen, supratentoriellen PNET und intrakraniellen Keimzelltumoren im Kindesalter

In most countries, residential electricity consumption typically ranges from 20% to 40% of total electricity consumption. This energy is used for heating, cooling, refrigeration and other end-uses. Significant energy savings are possible if new appliance purchases are for models with higher efficiency than that of existing models. There are several ways to ensure or encourage such an outcome, for example, appliance rebates, innovative procurement, and minimum efficiency standards. This paper focuses on the latter approach. At the present time, the US is the only country with comprehensive appliance energy efficiency standards. However, many other countries, such as Australia, Canada, the European Community (EC), Japan and Korea, are considering enacting standards. The greatest potential impact of minimum efficiency standards for appliances is in the developing countries (e.g., China and India), where ...

1993-03-01

348

Yeast allosteric chorismate mutase is locked in the activated state by a single amino acid substitution

Methods: Systemic irradiation of neuroaxis is an essential part in the management of medulloblastoma, stPNET and intracranial germ cell tumors. The introduction of quality assurance programs in radiooncology assures a precise radiotherapy of target volumes and is a prerequisite to improve survival. Results: Hyperfractionated radiotherapy has the potential of increasing dose to tumor more safely without increasing the risk for late adverse effects. Pilot studies revealed excellent tumor control in medulloblastoma with acceptable acute toxicity and a long-term survival of up to 96%. In medulloblastoma stereotactic radiation techniques reveal an acceptable toxicity and promising results in tumor control in recurrent disease or as primary treatment. They are now part of future treatment protocols in case of persisting residual tumor. Radiotherapy alone in pure germinoma is continuously yielding high cure rates. In secreting germ cell tumors cisplatin containing ...

2001-09-01

349

The effect of pulse voltage and capacitance on biosorption of uranium by biomass derived from whiskey distillery spent wash

Chorismate mutase, a branch-point enzyme in the aromatic amino acid pathway of Saccharomyces cerevisiae, and also a mutant chorismate mutase with a single amino acid substitution in the C-terminal part of the protein have been purified approximately 20-fold and 64-fold from overproducing strains, respectively. The wild-type enzyme is activated by tryptophan and subject to feedback inhibition by tyrosine, whereas the mutant enzyme does not respond to activation by tryptophan nor inhibition by tyrosine. Both enzymes are dimers consisting of two identical subunits of M_r 30,000, each one capable of binding one substrate and one activator molecule. Each subunit of the wild-type enzyme also binds one inhibitor molecule, whereas the mutant enzyme lost this ability. The enzyme reaction was observed by "1H NMR and shows a direct and irreversible conversion of chorismate to prephenate without the accumulation of any enzyme-free intermediates. The kinetic data of the wild-type chorismate mutase ...

350

Quality changes of irradiated spices stored in different indigenous packaging materials

Biosorption of uranium by residual biomass from The Old Bushmill`s Distillery Co. Ltd., Bushmills, Co. Antrim, Northern Ireland, following exposure to short and intense electric pulses has been examined. The biomass was prepared from the distillery spent wash and consisted of non-viable yeast and bacterial cells. As shown previously, untreated biomass had a maximum biosorption capacity of 170 mg uranium/g dry weight biomass. When biosorption reactions were placed between two electrodes and exposed to electric pulses with field strengths ranging from 1.25-3.25 kV/cm at a capacitance of 25 {mu}F, biosorption increased from 170 mg of uranium to 275 mg uranium/g dry weight biomass. The data were obtained from biosorption isotherm analyses and taken as the degree of biosorption at residual uranium concentrations of 3 mM. In addition, when the capacitance of the electric pulses increased from 0.25 {mu}F to 25 {mu}F at a fixed pulse field strength the degree of ...

1999-01-01

351

Multicolor FISH mapping with Alu-PCR-amplified YAC clone DNA determines the order of markers in the BRCA1 region on chromosome 17q12-q21

The changes in quality of irradiated spices, i.e., nutmeg (Myristica fragrans), black pepper and white pepper (Piper nigrum) stored in different indigenous packaging materials were evaluated. Packaging materials used were tin cans; tin can lined with polypropylene film, tin can lined with wrapping paper, woven polypropylene bag and woven polypropylene bag lined with polypropylene film. Packaging in woven polypropylene bag could cause significant changes in a_w-values and moisture contents of the spices. In the tin can, no significant change was found during storage. By applying a polypropylene film liner inside the plastic bag, the changes could be retarded. The other parameters observed, i.e. total plate count, total mould and yeast count and organoleptic scores of the samples were not significantly affected by the packaging method. A dose of 5 kGy could reduce the microbial load of the spices by about 2-3 log cycles. A significant decrease in a_w-value and ...

1990-03-01

352

Caldicellulosiruptor obsidiansis sp. nov., an anaerobic, extremely thermophilic, cellulolytic bacterium isolated from Obsidian Pool, Yellowstone National Park

A gene designated BRCA1, implicated in the susceptibility to early-onset familial breast cancer, has recently been localized to chromosome 17q12-q21. To date, the order of DNA markers mapped within this region has been based on genetic linkage analysis. The authors report the use of multicolor fluorescence in situ hybridization to establish a physically based map of five polymorphic DNA markers and 10 cloned genes spanning this region. Three cosmid clones and Alu-PCR-Generated products derived from 12 yeast artificial chromosome clones representing each of these markers were used in two-color mapping experiments to determine an initial proximity of markers relative to each other on metaphase chromosomes. Interphase mapping was then employed to determine the order and orientation of closely spaced loci by direct visualization of fluorescent signals following hybridization of three probes, each detected in a different color. Statistical analysis of the combined data ...

1993-09-01

353

Reappraisal of solid selective emitters

A novel, obligately anaerobic, extremely thermophilic, cellulolytic bacterium, designated OB47T, was isolated from Obsidian Pool, Yellowstone National Park, WY, USA. The isolate was a non-motile, non-spore forming, Gram-positive rod approximately 2 m long by 0.2 m wide and grew at temperatures between 55-85oC with the optimum at 78oC. The pH range for growth was 6.0-8.0 with values of near 7.0 being optimal. Growth on cellobiose produced the fastest specific growth rates at 0.75 hr-1. The organism also displayed fermentative growth on glucose, maltose, arabinose, fructose, starch, lactose, mannose, sucrose, galactose, xylose, arabinogalactan, Avicel, xylan, filter paper, processed cardboard, pectin, dilute acid-pretreated switchgrass and Populus. OB47T was unable to grow on mannitol, fucose, lignin, Gelrite, acetate, glycerol, ribose, sorbital, carboxymethylcellulose and casein. Yeast extract stimulated growth and thiosulfate, sulfate, nitrate, and sulfur were not ...

2010-02-01

354

Lasing efficiency and tuning range of a dynamic distributed-feedback laser

New rare earth oxide emitters show greater efficiency than previous emitters. As a result, based on a simple model the efficiency of these emitters was calculated. Results indicate that the emission band of the selective emitter must be at relatively low energy (less than or equal to .52 eV) to obtain maximum efficiency at moderate emitter temperatures (less than or equal to 1500 K). Thus low bandgap energy PV materials are required to obtain an efficient thermophotovoltaic (TPV) system. Of the 4 specific rare earths (Nd, Ho, Er, Yb) studied Ho has the largest efficiency at moderate temperatures (72 percent at 1500 K). A comparison was made between a selective emitter TPV system and a TPV system that uses a thermal emitter plus a band pass filter to make the thermal emitter behave like a selective emitter. Results of the comparison indicate that only for very optimistic filter and ...

1990-05-01

355

Characterization of 3D thermal neutron semiconductor detectors

The effects of the contrast of the pump field interference pattern, that is, the effectiveness of the dynamic amplitude-phase grid, and various optical arrangements on the lasing efficiency and tuning range of a dynamic distributed-feedback dye laser are investigated. It is shown that the proper choice of prism apex angle, prism material and dye solvent in a laser in the range 400-1000 microns pumped by two beams of different intensity directed through the congruent sides of an isosceles prism in contact with the active medium solution on its third side can lead to optical losses of less than 2% from the boundaries of the prism. Measurements of polymethine dye laser efficiency and tuning range as a function of the relative intensity of the two ruby-laser pump beams reveals that as the contrast of the pump beam interference pattern decreases, the tuning range remains practically constant and the efficiency increases in the ...

1980-08-01

356

The effects of one- and two-stage orchiopexy on postoperative serum testosterone levels and testicular volume in adult patients with bilateral nonpalpable testes.

Neutron semiconductor detectors for neutron counting and neutron radiography have an increasing importance. Simple silicon neutron detectors are combination of a planar diode with a layer of an appropriate neutron converter such as 6LiF. These devices have limited detection efficiency of not more than 5%. The detection efficiency can be increased by creating a 3D microstructure of dips, trenches or pores in the detector and filling it with a neutron converter. The first results related to the development of such devices are presented. Silicon detectors were fabricated with pyramidal dips on the surface covered with 6LiF and then irradiated by thermal neutrons. Pulse height spectra of the energy deposited in the sensitive volume were compared with simulations. The detection efficiency of these devices was about 6.3%. Samples with different column sizes were fabricated to study the electrical properties of 3D structures. ...

2007-06-11

357

Regulation of the phosphoinositide pathway in cultured Sertoli cells from immature rats: effects of follicle-stimulating hormone and fluoride

In this study, we investigated the effects of one- and two-staged orchiopexy on testicular volume and serum testosterone levels in cases with high, abdominally localized, bilateral nonpalpable testes. Between March 1996 and April 2001, orchiopexy was performed on 46 testes in 23 patients with bilateral nonpalpable testes. In 15 of the 23 patients, a two-stage Fowler-Stephens orchiopexy was performed, and in 8 of the 23 patients, a one-stage laparoscopic orchiopexy was performed. For one patient who lacked both testes, bilateral prosthetic testes were inserted. The patients' ages were between 20 and 23 years (average, 21 years). In the first stage of the two-stage orchiopexy, the spermatic artery was ligated laparoscopically. Six months later, open surgery orchiopexy was performed. Patients were followed for 2 to 16 months (average, 10 months) and were evaluated for testicular atrophy, serum testosterone levels, and complications. In all the patients, the preoperative secondary sexual ...

2002-10-01

358

Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes

Many hormones elicit effects on target cells by stimulating the enzyme phospholipase-C, which catalyzes the hydrolysis of phosphoinositides to the intracellular second messengers diacylglycerol and inositol phosphates. The present study examined the roles of FSH and guanine nucleotide-binding proteins (G-proteins) in regulating the hydrolysis of phosphoinositides in Sertoli cells. Sertoli cell cultures prepared from 16- to 18-day-old rats were incubated for 24 h with myo-[2-3H] inositol to label endogenous phospholipids. Treatment of cells from 0.5-20 min with preparations of ovine FSH ranging in potency from 1-60 times that of NIH FSH S1 did not affect accumulation of inositol phosphates. Levels of total [3H]inositol phosphates [[3H]inositol mono-, di-, and triphosphates (IP, IP2, and IP3)] in FSH-treated cultures was 75-120% the levels in control cultures over the various time intervals studied. Addition of testosterone and the combination of testosterone plus retinoic acid, agents ...

359

Pteromalus puparum venom impairs host cellular immune responses by decreasing expression of its scavenger receptor gene.

To study the regulation of adipose tissue lipoprotein lipase (LPL) in human adipocytes, omental adipose tissue was obtained from healthy subjects and digested in collagenase. The isolated adipocytes thus obtained were suspended in Medium 199 and cultured at 37 degrees C. Cell viability was demonstrated in adipocytes cultured for up to 72 h by constancy of cell number, cell size, trypan-blue exclusion, and specific /sup 125/I-insulin binding. In addition, chloroquine induced an increase in cell-associated /sup 125/I-insulin at 24, 48, and 72 h after preparation. Thus, isolated adipocytes retained their ability to bind, internalize, and degrade insulin. LPL was measured as activity secreted into the culture medium (CM), released from cells by heparin (HR), and extracted from cell digests. A broad range of heparin concentrations produced a prompt release of LPL from a rapidly replenishable pool of cellular activity. When cells were cultured in medium containing 10% ...

1985-01-01

360

Photoaffinity labeling of ATP and NAD"+ binding sites on recombinant human interleukin 2

Insect host/parasitoid interactions are co-evolved systems in which host defenses are balanced by parasitoid mechanisms to disable or hide from host immune effectors. Although there is a rich literature on these systems, parasitoid immune-disabling mechanisms have not been fully elucidated. Here we report on a newly discovered immune-disabling mechanism in the Pieris rapae/Pteromalus puparum host/parasitoid system. Because venom injections and parasitization suppresses host phagocytosis, we turned attention to the P. rapae scavenger receptor (Pr-SR), posing the hypothesis that P. puparum venom suppresses expression of the host Pr-SR gene. To test our hypothesis, we cloned a full-length cDNA of the Pr-SR. Multiple sequences alignment showed the deduced amino acid sequence of Pr-SR is similar to scavenger receptors of other lepidopterans. Bacterial and bead injections induced Pr-SR mRNA and protein expression, which peaked at 4h post-bead injection. Venom injection inhibited Pr-SR ...

2011-07-22

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

361

On the cells of origin of radiogenic thyroid cancer: New studies based on an old idea

Interleukin 2 (IL-2) is a T-cell-derived lymphokine critical in the activation and proliferation of T cells, B cells, and lymphokine-activated killer cells. It is a glycoprotein of #approx#15,500 daltons that is synthesized and secreted after activation by antigen or mitogen. By using the analogs 8-azidoadensoine 5'-[#gamma#-"3"2P]triphosphate ([#gamma#-"3"2P]8N_3ATP) and nicotinamide 2-azidoadenine [adenylate-"3"2P]dinucleotide ([#alpha#-"3"2P]2N_3NAD"+) as photoaffinity probes, the authors have detected specific, metal ion-requiring nucleotide binding sites on recombinant human IL-2 (rhIL-2). The specificity of these nucleotide interactions with rhIL-2 was demonstrated by saturation effects and by competition by the parent nucleotides at physiologically relevant concentrations. Saturation of photoinsertion into rhIL-2 occurred at 50 #mu#M [#gamma#-"3"2P]8N_3ATP. Saturation of photoinsertion with [#alpha#-"3"2P]2N_3NAD"+ was observed at 180 #mu#M. The extent of ...

362

Localisation of the neuropeptide PACAP and its receptors in the rat parathyroid and thyroid glands.

We have presented evidence that the functional thyroid follicles (follicular units, FU) which are formed in grafts of monodispersed rat thyroid cells, and hence the thyroid tumors which later develop in such grafts, are clonal in origin. Recent studies have been designed to investigate: whether cell number-dependent inhibition of promotion-progression is mediated by remote hormonal feed-back, local cell-cell interactions, or both; the cell population kinetics of the clonogen subpopulation during goitrogenesis and goiter involution; and the effect of prolonged exposure to high levels of TSH (thyrotropin) on the capacity of the clonogens to give rise to functional FU. The results indicate that local cell-cell interactions play an important role in the cell number-dependent suppression of neoplastic promotion-progression. They also show that if sufficient thyroid cells are grafted, the thyroid-pituitary axis can be reestablished in thyroidectomized rats fed normal diets. In such animals ...

1990-01-01

363

Inhibitory effect of transforming growth factor-. beta. (TGF-. beta. ) on insulin-like growth factor 1 (IGF-1)-induced proliferation and differentiation in primary cultures of pig preadipocytes

PACAP (pituitary adenylate cyclase activating polypeptide) is widely distributed neuropeptide acting via three subtypes of receptors, PAC(1), VPAC(1) and VPAC(2). Here we examined the localisation and nature of PACAP-immunoreactive nerves in the rat thyroid and parathyroid glands and defined the distribution of PAC(1), VPAC(1) and VPAC(2) receptor mRNA's. In the parathyroid gland a large number of nerve fibres displaying PACAP-immunoreactivity were distributed beneath the capsule, around blood vessels and close to glandular cells. Most of the PACAP-nerves were sensory, since they co-stored CGRP (calcitonin-gene-related peptide) and were sensitive to capsaicin-treatment. mRNA's for PAC(1) and VPAC(2) receptors occurred in the parathyroid gland, mainly located in the glandular cells. In the thyroid gland PACAP-immunoreactive nerve fibres were associated with blood vessels, thyroid follicles and parafollicular C-cells. A high degree of co-existence between PACAP and VIP (vasoactive ...

2010-12-19

364

Inhibitory effect of transforming growth factor-#beta# (TGF-#beta#) on insulin-like growth factor 1 (IGF-1)-induced proliferation and differentiation in primary cultures of pig preadipocytes

The influence of serum, IGF-1 and TGF-{beta} on the differentiation of preadipocytes was examined in primary cultures of porcine adipose tissue cells. In serum-supplemented or serum-free, IGF-1 (1 and 10 nM) had no effect on total cell number. However, IGF-1 (10nM) increased adipocyte number only in serum-supplemented (1% pig serum) cultures, whereas TGF-{beta} (15 pm) reduced the adipocyte number in the presence and absence of IGF-1. Replication of preadipocytes was analyzed with a ({sup 3}H) thymidine assay. Preadipocyte proliferation (cpm in adipocyte fraction) was increased by IGF-1 (10nM) only in cultures containing pig serum. TGF-{beta} had no effect on preadipocyte proliferation specifically, but slightly increased total ({sup 3}H) thymidine incorporation in cultures with serum. Glycerol phosphate dehydrogenase (GPDH) specific activity was decreased by adding TGF-{beta} to serum-free cultures but TGF-{beta} had little effect in serum-supplemented cultures. Cellular ...

1990-02-26

365

Inactivating calcium-sensing receptor mutations in patients with primary hyperparathyroidism.

The influence of serum, IGF-1 and TGF-#beta# on the differentiation of preadipocytes was examined in primary cultures of porcine adipose tissue cells. In serum-supplemented or serum-free, IGF-1 (1 and 10 nM) had no effect on total cell number. However, IGF-1 (10nM) increased adipocyte number only in serum-supplemented (1% pig serum) cultures, whereas TGF-#beta# (15 pm) reduced the adipocyte number in the presence and absence of IGF-1. Replication of preadipocytes was analyzed with a ["3H] thymidine assay. Preadipocyte proliferation (cpm in adipocyte fraction) was increased by IGF-1 (10nM) only in cultures containing pig serum. TGF-#beta# had no effect on preadipocyte proliferation specifically, but slightly increased total ["3H] thymidine incorporation in cultures with serum. Glycerol phosphate dehydrogenase (GPDH) specific activity was decreased by adding TGF-#beta# to serum-free cultures but TGF-#beta# had little effect in serum-supplemented cultures. Cellular ...

1991-04-21

366

Immunological detection and quantification of DNA components structurally modified by alkylating carcinogens, mutagens and chemotherapeutic agents

Objective:? Primary hyperparathyroidism (HPT) is characterised by autonomous secretion of PTH from enlarged parathyroid glands leading, in most patients, to asymptomatic hypercalcaemia. Familial hypocalciuric hypercalcaemia (FHH) is an autosomal dominant disorder caused by inactivating mutations in the calcium sensing receptor (CaSR) gene; it is characterised by lifelong and usually asymptomatic hypercalcaemia. Establishing the correct diagnosis is important because surgery can be curative in HPT, but ineffective in FHH. There is overlap in the diagnostic criteria for the two disorders and some patients carrying inactivating mutations in the CaSR gene, which is suggestive of FHH, also have HPT with hyperplastic parathyroid glands or adenomas. Design and Patients:? CaSR gene mutations were analyzed and clinical and biochemical parameters evaluated in 139 consecutive out-patients presenting with hypercalcaemia and suspected of having HPT. Results:? Six different ...

2011-03-29

367

Genome Sequence of a Lancefield Group C Streptococcus zooepidemicus Strain Causing Epidemic Nephritis: New Information about an Old Disease

The detection and quantification of defined reaction products of chemical mutagens and carcinogens (and of many cancer chemotherapeutic agents) with DNA require highly sensitive analytical techniques. The exceptional capability of immunoglobulins to recognize subtle alterations of molecular structure (especially when monoclonal antibodies are used to maximize specificity), outstanding sensitivity of immunoanalysis by high-affinity antibodies, and the fact that radioactively-labelled agents are not required suggest the utility of a radioimmunoassay to recognize and quantitate alkylated DNA products. We have recently developed a set of high-affinity monoclonal antibodies (secreted by mouse x mouse as well as by rat x rat hybridomas; antibody affinity constants, 10"9 to > 10"1"0 lmol) specifically directed against several DNA alkylation products with possible relevance in relation to both mutagenesis and malignant transformation of mammalian cells. These alkylation ...

1983-09-01

368

Expression of Na(+)-H+ exchange and ATP-dependent proton extrusion in growing rat IMCD cells

Outbreaks of disease attributable to human error or natural causes can provide unique opportunities to gain new information about host-pathogen interactions and new leads for pathogenesis research. Poststreptococcal glomerulonephritis (PSGN), a sequela of infection with pathogenic streptococci, is a common cause of preventable kidney disease worldwide. Although PSGN usually occurs after infection with group A streptococci, organisms of Lancefield group C and G also can be responsible. Despite decades of study, the molecular pathogenesis of PSGN is poorly understood. As a first step toward gaining new information about PSGN pathogenesis, we sequenced the genome of Streptococcus equi subsp. zooepidemicus strain MGCS10565, a group C organism that caused a very large and unusually severe epidemic of nephritis in Brazil. The genome is a circular chromosome of 2,024,171 bp. The genome shares extensive gene content, including many virulence factors, with genetically related group A ...

2008-08-21

369

Development of an assay for a biomarker of pregnancy and early fetal loss

As the last step of urinary acidification, the inner medullary collecting duct (IMCD) is thought to secrete protons into the tubular lumens by means of a H(+)-translocating adenosinetriphosphatase (H(+)-ATPase). However, recent studies have also shown the existence of Na(+)-H+ exchange activity in IMCD cells. Although the physiological function of the antiporter in IMCD cells is unknown, activation of Na(+)-H+ exchange in other cell-culture systems has been suggested to be closely associated with the process of cell growth. Thus presence of Na(+)-H+ exchange may relate to the growth phase of these cells. To examine intracellular pH (pHi) regulation in growing IMCD cells, we studied proton transport by Na(+)-dependent and Na(+)-independent mechanisms by microfluorimetry using the pHi-sensitive dye 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein acetoxymethyl ester (BCECF/AM). Actively growing cells, defined by (3H)thymidine incorporations, ...

1990-03-01

370

Characterization of monocyte differentiation-inducing (MDI) factors derived from human fetal membrane chorion cells undergoing apoptosis after influenza virus infection.

Human chorionic gonadotropin (hCG) is a glycoprotein hormone, secreted by the syncytiotrophoblast cells of the fertilized ovum, that enters the maternal circulation at the time of endometrial implantation. It is composed of two nonidentical subunits; ..cap alpha.. and ..beta.., with molecular weights of 14 kD and 23 kD, respectively. Human chorionic gonadotropin binds to the same receptor as hLH and displays the same biological response, namely, to stimulate the declining function of the corpus luteum to produce progestins and estrogen late in the menstrual cycle. The differences in the structures of hCG and hLH have been exploited to develop antibodies that can measure hCG specifically in the presence of hLH. Two-site antibody binding assays have been developed, based on a surface immunological concept of hCG epitopes, that involve four distinct regions to which antibodies against hCG can bind simultaneously. Antibody cooperative effects, in conjunction with ...

1987-10-01

371

Thermodynamic analysis of a hybrid geothermal heat pump system

Influenza virus infection during pregnancy has been implicated as one of cause of premature delivery, abortion and stillbirth. We have reported that cultured human fetal membrane chorion cells undergoing apoptosis by influenza virus infection secrete unidentified heat-stable monocyte differentiation-inducing (MDI) factors. In this study, cellular, biological and immunochemical characteristics of MDI factors were investigated using human monocytic leukemia THP-1 cells by nitroblue tetrazolium reduction and cell adhesion assays. The treatment of THP-1 cells with culture supernatants from the influenza virus-infected chorion cells induced the nitroblue tetrazolium reduction ability, which was inhibited by the addition of superoxide dismutase and diphenyleneiodonium chloride, an inhibitor for reduced nicotinamide adenine dinucleotide phosphate oxidase. The phenomenon was also observed in human peripheral blood monocytes and histiocytic leukemia U937 cells, but not in ...

2006-06-06

372

British Library Electronic Table of Contents (United Kingdom)

A thermodynamic analysis of a hybrid geothermal heat pump system is carried out. Mass, energy, and exergy balances are applied to the system, which has a cooling tower as a heat rejection unit, and system performance is evaluated in terms of coefficient of performance and exergy efficiency. The heating coefficient of performance for the overall system is found to be 5.34, while the corresponding exergy efficiency is 63.4%. The effect of ambient temperature on the exergy destruction and exergy efficiency is investigated for the system components. The results indicate that the performance of hybrid geothermal heat pump systems is superior to air-source heat pumps.

2011-01-01

373

Thermal-receiver designs for line-focus solar collectors

The effect of temperature on the radiative performance of Ho-YAG thin film selective emitters

Considerable potential exists for increasing the annual efficiency of line-focus collectors by reducing receiver heat losses. Five alternate receiver designs are compared with a standard design. The comparison is based on annual collector-field efficiency using optimized receiver designs. Of the receivers studied, the all-glass design and the cavity design provide the greatest increases in efficiency. Average fluid temperatures range from 200/sup 0/ to 450/sup 0/C. As a guide for further study, areas of uncertainty and potential problems are identified.

1982-02-01

374

The Integration of Energy Efficiency, Renewable Energy, DemandResponse and Climate Change: Challenges and Opportunities for Evaluatorsand Planners

The authors present the emitter efficiency results for the thin film 25 percent Ho YAG (Yttrium Aluminum Garnet, Y3Al5O12) selective emitter from 1000 to 1700 K with a platinum substrate. Spectral emittance and emissive power measurements were made (1.2 less than lambda less than 3.2 microns) and used to calculate the radiative efficiency. The radiative efficiency and power density of rare earth doped selective emitters are strongly dependent on temperature and experimental results indicate an optimum temperature (1650 K for Ho YAG) for thermophotovoltaic (TPV) applications.

1995-01-01

375

Sod peat harvesters - results of time and yield research in summer 1986

This paper explores the feasibility of integrating energyefficiency program evaluation with the emerging need for the evaluationof programs from different "energy cultures" (demand response, renewableenergy, and climate change). The paper reviews key features andinformation needs of the energy cultures and critically reviews theopportunities and challenges associated with integrating these withenergy efficiency program evaluation. There is a need to integrate thedifferent policy arenas where energy efficiency, demand response, andclimate change programs are developed, and there are positive signs thatthis integration is starting to occur.

2007-05-29

376

Performance estimates of photoneutralized negative-ion beams

More efficient and safe production machines are required for sod peat production. The efficiency and durability of machines is especially important in contract work. Two sod peat harvester types, Pala-14 and Turve-Unsa, were tested by the forest department of the Work Efficiency Associationin summer 1986. Sod peat was collected with these machines at three sod peat production sites. The yield of the Pala-14 harvester was 121 m/sup 3//h for harvesting time, and 56 m/sup 3//h for worksite time, and that of Turve-Unsa was 108 m/sup 3//h and 55 m/sup 3//h, respectively.

1986-01-01

377

Parameter optimization of a planar BEGe detector using Monte Carlo simulations

Recent studies have provided data that make it possible to estimate the efficiency and cost of future beamlines using a chemical oxygen-iodine laser as a neutralizer. These studies indicate that a 400-keV neutral deuterium beam of more than 20 A will operate at an efficiency >60%, with the capital cost of the neutralizer at less than $2/W of neutral beam output. Beamlines of lower current and less energy will operate at poorer efficiencies and higher neutralizer costs per watt of neutral beam. These are estimates. As they are very sensitive to changes in the assumptions from which they were derived, they must be used with some caution. Additional studies are expected to provide more reliable estimates.

1984-11-01

378

Optimized hydrogen piston engines

This work reports on the use of three state-of-the-art Monte Carlo codes (MCNPX, PENELOPE, FLUKA) in the efficiency calibration of a Broad-Energy Germanium (BEGe) detector. Initial discrepancies found between the experimental and computational efficiency values are related to the poor knowledge of some physical parameters of the detector (dead-layers, crystal dimensions, etc.). As a consequence, a sensitivity analysis was carried out. Each parameter was systematically analyzed, and an accurate model of the detector was determined. The obtained results are consistent, allowing this model to be used in computational efficiency calibrations of the equipment at stake.

2010-11-21

379

High efficiency GaInP/GaAs tandem solar cells

Hydrogen piston engines can be simultaneously optimized for improved thermal efficiency and for extremely low emissions. Using these engines in constant-speed, constant-load systems such as series hybrid-electric automobiles or home cogeneration systems can result in significantly improved energy efficiency. For the same electrical energy produced, the emissions from such engines can be comparable to those from natural gas-fired steam power plants. These hydrogen-fueled high-efficiency, low-emission (HELE) engines are a mechanical equivalent of hydrogen fuel cells. HELE engines could facilitate the transition to a hydrogen fuel cell economy using near-term technology.

1994-05-10

380

High capacity heavy media processing

We report on multijunction GaInP/GaAs photovoltaic cells with total-area efficiencies of 29.5% at one-sun concentration and air mass (AM) 1.5 global and 25.7% one-sun, AM0. These values represent the highest efficiencies achieved by any solar cell under these illumination conditions. Three key areas in this technology are identified and discussed: the grid design, front surface passivation of the top cell, and bottom surface passivation of both cells. Aspects of cell design related to its operation under different solar spectra and under concentration are also discussed.

1994-06-30

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

381

GaInP/GaAs monolithic tandem concentrator cells

Pittston Coal Company recognized the potential economic benefit of improving process efficiency with increased capacity at it`s U.K. No. 1 Coal Preparation Plant. Accordingly, extensive research, both domestically and internationally, was conducted to select the appropriate technologies to achieve the desired circuit in the most cost effective manner while, at the same time, sacrificing neither structural integrity nor process performance. Large diameter heavy media cyclone technology was combined with highly efficient banana type screens and improved high intensity magnetic separators to create a coordinated process circuit that provided high efficiency at very high capacities in an economically attractive package.

1996-12-31

382

Flue gas desulfurization. Rauchgasentschwefelung

This paper discusses design considerations for the recently introduced GaInP/GaAs monolithic tandem concentrator cell. The prototype device achieves a peak efficiency of 30.2% in a range of 140--180 suns, making this the first two-terminal device to demonstrate a verified efficiency exceeding 30%. At 425 suns the efficiency is still above 29%. The authors focus on the issues of grid design, top-cell thickness, and antireflectance coat. They also examine ways in which these aspects of the device may be modified to provide further performance improvements for future devices.

1994-12-31

383

Engineering study on steam storage power generation. System screening and efficiency

Low-dust flue gases can be washed efficiently of SO/sub 2/, and the flue gas heat can be utilized. The residual heat fraction still required by the process is characterized by a temperature of 15/sup 0/K above the temperature of the purified flue gas. Marketable crystalline ammonium sulfate is produced. The high efficiency and low heat consumption are achieved by combining all process stages in a single, fully integrated cycle process free of heat and materials losses. The process is characterized by high efficiency, low investment and operating costs, recovery of thermal energy, and production of an ammonium sulfate fertilizer.

1983-04-07

384

Efficient capture of nonlinear oscillations into resonance

Large scale steam storage power generation, one of the new energy storage systems for the future of inflexible electric power sources consisting of nuclear and coal power plants has been studied on the subjects of the systems to be attached to coal and nuclear power units, of the definition of storage efficiency and of the vertical steam storage vessel technology. Steam storage power generation may be hopeful for its higher efficiency similarly defined as of pumped storage plants while high temperature heat storage and the internal structure of large vertical steam storage vessel (accumulator) need to be developed.

1981-11-01

385

Computer aided monitoring of pump efficiency by using ART2 neural networks; ART2 nyurarunettowaku niyoru ponpuseino no rekka shindan shien

The problem of efficient capture of nonlinear oscillations into resonance is discussed. The capture is guaranteed by passage through resonance when the system starts in equilibrium and the driving amplitude exceeds a threshold. The threshold problem is described by a universal nonlinear Schroedinger-type equation with a single parameter and cannot be analyzed by perturbation methods. A similar threshold phenomenon is a characteristic of two weakly coupled oscillators with a slow parameter if one of the oscillators starts in equilibrium, allowing efficient capture into resonance and subsequent adiabatic (autoresonant) control of strongly excited nonlinear oscillations.

2008-10-17

386

An efficient control variate method for pricing variance derivatives

As an application of ART2 neural networks, computer aided monitoring of pump efficiency is successfully examined for an industrial waste-liquid treatment process with measured data of valve openness and liquid flow rates. By running the neural networks in parallel, we confirm that accuracy to detect system changes is good, and the adjustment of classifier parameters is relatively easy. Investigating the resulting classes carefully, frequency of each class is correlated with pump efficiency. The relative amount of variables are also related to the classes. (author)

2000-05-10

387

British Library Electronic Table of Contents (United Kingdom)

This paper studies the pricing of variance swap derivatives with stochastic volatility by the control variate method. A closed form solution is derived for the approximate model with deterministic volatility, which plays the key role in the paper, and an efficient control variate technique is therefore proposed when the volatility obeys the log-normal process. By the analysis of moments for the underlying processes, the optimal volatility function in the approximate model is constructed. The numerical results show the high efficiency of our method; the results coincide with the theoretical results. The idea in the paper is also applicable for the valuation of other types of variance swap, options with stochastic volatility and other financial derivatives with multi-factor models.

2010-01-01

388

Transport energy efficiency

The effect of swirl ratio and fuel injection parameters on CO emission and fuel conversion efficiency for high-dilution, low-temperature combustion in an automotive diesel engine.

Enhancing transport sustainability is the password of national, European, and international policies. The development of innovative solutions is ranging from bio fuels to transport analysis models, materials, traction technologies, mobility management, freight villages.

389

Strategy for population protection at the end of the emergency phase: the C.o.d.i.r.p.a. view; Strategie de protection des populations a l'issue de la phase d'urgence: reflexions du Codirpa

No abstract prepared.

2006-01-01

390

Strategy for population protection at the end of the emergency phase: the C.o.d.i.r.p.a. view

In the case of a serious accident and as a precautionary measure, several protection actions can be implemented by the prefect during the emergency phase. In France the levels of intervention are given by a ministerial decree of the 13. october 2003, resuming recommendations of the International Atomic Energy Agency. The selected values are: an efficient dose of 10 mSv for the stake under cover and in the listening: the concerned persons have to put under cover in a building, any openings carefully closed and stay in the listening of the orders of the prefect; an efficient dose of 50 mSv for evacuation; an efficient dose to the thyroid of 100 mSv for the taking of stable iodine tablets. In order to help the authorities to choose between the keeping of populations at their place or immediate evacuation the decision criteria are: if the forecasted efficient dose of the first month is under 1 mSv, the ...

2008-07-15

391

Sleeping Beauty Transposition From Nonintegrating Lentivirus

In the case of a serious accident and as a precautionary measure, several protection actions can be implemented by the prefect during the emergency phase. In France the levels of intervention are given by a ministerial decree of the 13. october 2003, resuming recommendations of the International Atomic Energy Agency. The selected values are: an efficient dose of 10 mSv for the stake under cover and in the listening: the concerned persons have to put under cover in a building, any openings carefully closed and stay in the listening of the orders of the prefect; an efficient dose of 50 mSv for evacuation; an efficient dose to the thyroid of 100 mSv for the taking of stable iodine tablets. In order to help the authorities to choose between the keeping of populations at their place or immediate evacuation the decision criteria are: if the forecasted efficient dose of the first month is under 1 mSv, the ...

2008-07-01

392

STS067-703D-090 Kinshasa ... - Earth from Space - Image Information

Lentiviral vectors enter cells with high efficiency and deliver stable transduction through integration into host chromosomes, but their preference for integration within actively transcribing genes...Full Text Available

2009-07-01

393

New III-V cell design approaches for very high efficiency. Annual subcontract report, 1 August 1990--31 July 1991

... rivers of the world, the Zaire is not an efficient transportation link because of many rapids and falls to negotiate between Kinshasa and the port city of Matadi. ...

394

New III-V cell design approaches for very high efficiency

This report describes progress during the first year of a three-year project. The objective of the research is to examine new design approaches for achieving very high conversion efficiencies. The program is divided into two areas. The first centers on exploring new thin-film approaches specifically designed for III-V semiconductors. The second area centers on exploring design approaches for achieving high conversion efficiencies without requiring extremely high quality material. Research activities consisted of an experimental study of minority carrier recombination in n-type, metal-organic chemical vapor deposition (MOCVD)-deposited GaAs, an assessment of the minority carrier lifetimes in n-GaAs grown by molecular beam epitaxy, and developing a high-efficiency cell fabrication process.

1993-01-01

395

Nail-Gun Injuries to the Hand

This report describes progress during the first year of a three-year project. The objective of the research is to examine new design approaches for achieving very high conversion efficiencies. The program is divided into two areas. The first centers on exploring new thin-film approaches specifically designed for III-V semiconductors. The second area centers on exploring design approaches for achieving high conversion efficiencies without requiring extremely high quality material. Research activities consisted of an experimental study of minority carrier recombination in n-type, metal-organic chemical vapor deposition (MOCVD)-deposited GaAs, an assessment of the minority carrier lifetimes in n-GaAs grown by molecular beam epitaxy, and developing a high-efficiency cell fabrication process.

1993-01-01

396

Background: The nail gun is a commonly utilized tool in carpentry and construction. When used properly with appropriate safety precautions, it can facilitate production and boost efficiency;...Full Text Available

397

NAME=\\

Molecular spectroscopy and planetary atmospheres

... These tankers will be converted to a dual-fuel engine to enable them to run efficiently on the biomethane. The other half will be conditioned and injected into National Gridrsquo;s gas distribution network. This will provide enough renewable energy for all the heating and ...

398

Modeling the Internet's large-scale topology

With the improvements accomplished during the past 15 years in detection techniques and instrumentation and with the opening of space exploration, molecular spectroscopy has become a very efficient way to probe planetary atmospheres.

1984-01-01

399

Measurements of environmental policy for air pollution abatement. Umweltpolitische Massnahmen zur Luftreinhaltung; Kosten-Nutzen-Analyse

Network generators that capture the Internet's large-scale topology are crucial for the development of efficient routing protocols and modeling Internet traffic. Our ability to design realistic generators...Full Text Available

2002-10-15

400

Maximum economic efficiency. Industriepark Hoechst utilizes biogas from liquid effluents of the pharmaceutical industry; ''Wirtschaftlich der guenstigste Weg''. Industriepark Hoechst nutzt Biogaspotenzial aus Pharmaabwaessern

The first part of the study goes into the determination of efficient strategies for the reduction of air pollutants. The developed method is not only derived theoretically but is tested with the concrete example of emissions sources of a German state. The second part goes into the question what the government can do in order to attain that air pollution abatement measures recognized as being efficient will be put into practice. As market economy mechanisms have advantages over central state planning in the allocation of economic resources the question arises if not also for environmental protection market economy tools may contribute to an improvement of the efficiency of air pollution abatement. Therefore the suitability of different tools of environmental policy for the realization of efficient air pollution abatement is investigated and evaluated. This is again not done abstractly but with existing ...

1993-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

401

Magnetic refrigeration: the promise and the problems

No abstract prepared.

2003-05-01

402

Low cost fabrication of sheet structure using a new beta titanium alloy, Ti-15V-3Cr-3Al-3Sn

Magnetic refrigeration uses the temperature- and field-dependence of the entropy of some magnetic materials to accomplish cooling. Because of the intrinsically high efficiency of the magnetization and demagnetization process and because of the potential for excellent heat transfer between solids and fluids, magnetic refrigerators promise to have higher efficiency than existing gas-cycle refrigerators. Many ground-based and space-based applications could benefit significantly from the cost savings implied by higher efficiency. Other attributes of these devices are high reliability and low volume and mass per unit cooling power. The development of these refrigerators is underway at several places around the world, including the Los Alamos National Laboratory. The progress to date has been encouraging but some problems have been clearly identified. The arguments for high efficiency and the problems that ...

1984-01-01

403

Introducing N-glycans into natural products through a chemoenzymatic approach**

Development efforts have been undertaken to improve the processing and structural efficiencies of

1982-01-01

404

International Energy Agency (IEA) Small Solar Power Systems (SSPS) sodium cavity and external receiver performance comparison

The present study describes an efficient chemoenzymatic method for introducing a core N-glycan of glycoprotein origin into various lipophilic natural products. It was found...Full Text Available

2008-11-24

405

Industrial Technologies Program: Chemicals

This report uses experimental data to compare the performance of two sodium cooled solar central receivers operated at the International Energy Agency Small Solar Power Systems project near Almeria, Spain. Performance includes point-in-time steady state efficiency, average efficiency, start-up time, and operation time. Point-in-time steady state efficiency calculations were based on the statistical method of least squares using receiver incident and absorbed powers. One receiver, a cavity type, showed a peak steady state receiver efficiency of 87% +- 5% and an average efficiency of about 67%. The other receiver, an external ''billboard'' type, had a peak steady state receiver efficiency of 96% +- 4% and an average efficiency of about 79%. The original design peak steady state ...

1987-10-01

406

Improved micropower logic ... - NASA Technical Report Server (NTRS)

Efficiency and Renewable Energy EERE Home | Programs & Offices | Consumer Information Chemicals Search Search Help Chemicals EERE » Industrial Technologies Program » Chemicals...

2011-10-01

407

Hurst exponent and prediction based on weak-form efficient market hypothesis of stock markets

4), is quite efficient for multivibrators because complementary outputs are available ... age or ground, much as is done in the complementary multivibrators. ...

408

High efficiency chemical energy conversion system based on a methane catalytic decomposition reaction and two fuel cells. Part II. Exergy analysis

We empirically investigated the relationships between the degree of efficiency and the predictability in financial time-series data. The Hurst exponent was used as the measurement of the degree of efficiency, and the hit rate calculated from the nearest-neighbor prediction method was used for the prediction of the directions of future price changes. We used 60 market indexes of various countries. We empirically discovered that the relationship between the degree of efficiency (the Hurst exponent) and the predictability (the hit rate) is strongly positive. That is, a market index with a higher Hurst exponent tends to have a higher hit rate. These results suggested that the Hurst exponent is useful for predicting future price changes. Furthermore, we also discovered that the Hurst exponent and the hit rate are useful as standards that can distinguish emerging capital markets from mature capital markets.

2007-01-01

409

GTMO Portfolio - IRAD Funded - NASA

A methane catalytic decomposition reactor-direct carbon fuel cell-internal reforming solid oxide fuel cell (MCDR-DCFC-IRSOFC) energy system is highly efficient for converting the chemical energy of methane into electrical energy. A gas turbine cycle is also used to output more power from the thermal energy generated in the IRSOFC. In part I of this work, models of the fuel cells and the system are proposed and validated. In this part, exergy conservation analysis is carried out based on the developed electrochemical and thermodynamic models. The ratio of the exergy destruction of each unit is examined. The results show that the electrical exergy efficiency of 68.24% is achieved with the system. The possibility of further recovery of the waste heat is discussed and the combined power-heat exergy efficiency is over 80%. (author)

2010-10-01

410

FFTF reactor plant procedures plan

Apr 27, 2007... Bruce Gentry: Small, Efficient Direct Detection Doppler Receiver for UAV and Spaceborne measurement of Tropospheric Winds ...

411

FFTF reactor plant procedures plan

The document presented defines the plan to be used to coordinate the preparation, review, approval, and issuance of the operating procedure documents required to ensure safe and efficient operation of FFTF.

1972-01-01

412

Enzymatic engineering of the porcine genome with transposons and recombinases

The document presented defines the plan to be used to coordinate the preparation, review, approval, and issuance of the operating procedure documents required to ensure safe and efficient operation of FFTF.

413

BackgroundSwine is an important agricultural commodity and biomedical model. Manipulation of the pig genome provides opportunity to improve production efficiency, enhance disease...Full Text Available

414

Energy efficient wireless unicast routing alternatives for machine-to-machine networks

No abstract prepared.

2000-07-01

415

British Library Electronic Table of Contents (United Kingdom)

Machine-to-machine (M2M) communications is a new and rapidly developing technology for large-scale networking of devices without dependence on human interaction. Energy efficiency is one of the important design objectives for machine-to-machine network architectures that often contain multi-hop wireless subnetworks. Constructing energy-efficient routes for sending data through such networks is important not only for the longevity of the nodes which typically depend on battery energy, but also for achieving an environmentally friendly system design overall, which will be imperative as M2M networks scale in number of nodes as projected. The objective of this survey is to provide a comprehensive look into shortest-path based energy-efficient routing alternatives to provide a reference for sys...

2011-01-01

416

Energy Cost and Efficiency of a Demanding Combined ...

Efficient DSP Hardware Implementations for Navy RF Systems

... Wright, and Vogel, 1985; Patton, Vogel, Damo- shorter amount of time at the end of the 45-hour kosh, et al., 1989). Lifting strength was signifi- ...

1994-07-01

417

Efficiency study of different photovoltaic plant connection schemes under dynamic shading

... of the loop filter based on ... algorithms, including delta-sigma approaches, routinely uses double-precision floating point number representations for ...

2010-09-30

418

Effective and Efficient Training and Advising in Pakistan

An important growth in the power of the photovoltaic systems connected to a grid has recently been observed. In spite of the advances in module technology, the problems in the system design increased, especially regarding the surface of the earth they occupy. In this work we propose a complete model for plant simulation with different wiring diagrams and under dynamic shading. Results obtained from simulations showed that the configuration with the lowest performance was that of only one serial-parallel group, whereas the highest efficiency corresponded to a design of groups of modules in parallel connected then in series. In general, a higher efficiency was obtained diminishing the quantity of modules in series and increasing their number in parallel. The simulation model proposed allows exploring different alternatives of wiring modules and finding the most efficient configurations for photovoltaic plants of medium and ...

2010-06-15

419

Economic-efficiency considerations in restructuring electric markets

... the more the populace backs the TTP or, at ... the FATA, which does not wholly belong to Pakistan. ... While Congress authorizes and funds SA and the ...

2010-06-01

420

Comments on the Quantum Afterburner

In response to the Energy Policy Act of 1992 and the Federal Energy Regulatory Commission`s subsequent rulemaking on transmission access, many states are exploring options to restructure their electric industries. In their deliberations on restructuring, policymakers should consider (1) the reliability of the electric system; (2) income-distribution effects on ratepayers and utilities; (3) social consequences such as effects on energy conservation, renewable energy, and the environment; and (4) economic efficiency. We address economic-efficiency considerations in this study. Economic efficiency is important because it is one of the primary reasons that policymakers should consider restructuring in the first place: improving the electric-industry`s efficiency lowers costs and, hence, electric prices. In this study, we look at the sources of (in)efficiency in existing and proposed ...

1996-12-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

421

Can microRNAs act as biomarkers of aging?

A process has been proposed to increase the efficiency of an ideal Otto cycle via a quantum heat engine that has no cooler reservoir. We show that such a process is not feasible.

2007-01-01

422

Autologous blood pleurodesis: A good choice in patients with persistent air leak

Aging can be defined as a progressive decline in physiological efficiency regulated by an extremely complex multifactorial process. The genetic makeup of an individual appears to dictate this rate...Full Text Available

423

Assessing farming eco-efficiency: A Data Envelopment Analysis approach

AIM:The study compares the efficiency, side effects and complications of autologous blood pleurodesis with talcum powder and tetracycline.MATERIALS...Full Text Available

2009-10-01

424

British Library Electronic Table of Contents (United Kingdom)

This paper assesses farming eco-efficiency using Data Envelopment Analysis (DEA) techniques. Eco-efficiency scores at both farm and environmental pressure-specific levels are computed for a sample of Spanish farmers operating in the rain-fed agricultural system of Campos County. The determinants of eco-efficiency are then studied using truncated regression and bootstrapping techniques. We contribute to previous literature in this field of research by including information on slacks in the assessment of the potential environmental pressure reductions in a DEA framework. Our results reveal that farmers are quite eco-inefficient, with very few differences emerging among specific environmental pressures. Moreover, eco-inefficiency is closely related to technical inefficiencies in the managemen...

2011-01-01

425

An efficient computational method for a stochastic dynamic lot-sizing problem under service-level constraints

British Library Electronic Table of Contents (United Kingdom)

We provide an efficient computational approach to solve the mixed integer programming (MIP) model developed by Tarim and Kingsman [8] for solving a stochastic lot-sizing problem with service level constraints under the static-dynamic uncertainty strategy. The effectiveness of the proposed method hinges on three novelties: (i) the proposed relaxation is computationally efficient and provides an optimal solution most of the time, (ii) if the relaxation produces an infeasible solution, then this solution yields a tight lower bound for the optimal cost, and (iii) it can be modified easily to obtain a feasible solution, which yields an upper bound. In case of infeasibility, the relaxation approach is implemented at each node of the search tree in a branch-and-bound procedure to efficiently sear...

2011-01-01

426

Adhoc Wireless Network Control: Energy Efficiency and ...

A fuel treatment reduces fire severity and increases suppression efficiency in a mixed conifer forest

... 3 Illustration of the hidden terminal problem in ... satellite downlink subject to stochastic power demands ... be immediately served, queueing effects are ...

2009-12-01

427

A Theoretical Solid Oxide Fuel Cell Model for Systems Controls and Stability Design

Fuel treatments are being implemented on public and private lands across the western United States. Although scientists and managers have an understanding ... ...

428

A New Model for the Organizational Structure of Medical Record Departments in Hospitals in Iran

As the aviation industry moves toward higher efficiency electrical power generation, all electric

2008-01-01

429

3-D depth migration via McClellan transformations

The organizational structure of medical record departments in Iran is not appropriate for the efficient management of healthcare information. In addition, there is no strong information management division...Full Text Available

430

l-Proline-catalyzed one-pot expeditious synthesis of highly substituted pyridines at room temperature

Three-dimensional seismic wavefields may be extrapolated in depth, one frequency at a time, by two-dimensional convolution with a circularly symmetric, frequency- and velocity-dependent filter. This depth extrapolation, performed for each frequency independently, lies at the heart of 3-D finite-difference depth migration. The computational efficiency of 3-D depth migration depends directly on the efficiency of this depth extrapolation. McClellan transformations provide an efficient method for both designing and implementing two-dimensional digital filters that have a particular form of symmetry, such as the circularly symmetric depth extrapolation filters used in 3-D depth migration. Given the coefficients of one-dimensional, frequency- and velocity-dependent filters used to accomplish 2-D depth migration, McClellan transformations lead to a simple and efficient algorithm for 3-D depth migration. 21 ...

1990-01-01

431

British Library Electronic Table of Contents (United Kingdom)

Only 15mol% of l-proline in ethanol proved to be a very efficient catalyst for the one-pot synthesis of a wide variety of highly substituted pyridines at room temperature. The methodology is mild, efficient, high yielding, and the products can be directly recrystallized from hot ethanol.

2010-01-01

432

X-ray generation by the Smith-Purcell effect

USDA Finances Wind for Rural Economic Development (Poster)

Smith-Purcell (S-P) radiation is produced when electrons graze the surface of a grating. Calculations based on the theory of diffraction radiation show that, given severe restrictions on e"--beam quality, S-P radiation is highly efficient. Efficient S-P x-ray generation requires relativistic e"- beams having a transverse momentum and dimension whose product approaches the Heisenberg uncertainty limit.

433

Toward efficient excavators

To foster rural economic development and growth, Congress passed the Renewable Energy Systems and Energy Efficiency Improvements Program as Section 9006 of the 2002 Farm Bill. This program provides financial assistance to farmers, ranchers, and rural small businesses to purchase renewable energy systems or make energy efficiency improvements. The Rural Business and Cooperative Services of the United States Department of Agriculture (USDA) administers this program. This conference poster provides an overview of Section 9006.

2005-05-01

434

Thermal performance of solar air collectors with a passive heat transfer augmentation technique

This article deals with excavation equipment used in open-pit mining operations. Because of competition in the mining industry, a demand for more energy-efficient excavators is being felt. The authors present different types of machinery and give statistics on energy use, weights, bucket capacity, and maximum digging depths. Actual manufacturers specifications are also given for different models. A short discussion on the role computers are playing in this new technology is also given.

1984-05-01

435

The Effect of Cocktail on Radiocarbon Analysis by Direct Absorption of Carbon dioxide

One of the main disadvantages of solar air collectors in practical applications is their relatively low efficiency. In this experimental investigation, the shape and arrangement of absorber surfaces of the collectors were reorganized to provide better heat transfer surfaces suitable for the passive heat transfer augmentation techniques. The performance of such solar air collectors with staggered absorber sheets was tested. The experimental results show that it is possible to provide 10 to 25% enhancement in the efficiency compared to the conventional solar air collectors. (authors)

2000-07-01

436

The AMOS cell - An improved metal-semiconductor solar cell

Carbon-14 was analysed by direct absorption of carbon dioxide on the mixture of organic amine compound and scintillation cocktail then counted by low level liquid scintillation counter. This method can be applied for radiocarbon dating. In this study, the effects of carbon dioxide absorption, and counting efficiency were investigated using various commercial available cocktail. It was found that permafluor V and Ready Gel showed the highest carbon dioxide absorption whereas the Permafluor V showed the highest counting efficiency

1998-12-01

437

Technical-ecologic principles of efficient use of open pit mine motor transport

A new fabrication process is being developed which significantly improves the efficiency of metal-semiconductor solar cells. The resultant effect, a marked increase in the open-circuit voltage, is produced by the addition of an interfacial layer oxide on the semiconductor. Cells using gold on n-type gallium arsenide have been made in small areas (0.17 sq cm) with conversion efficiencies of 15% in terrestrial sunlight.

1975-01-01

438

Sustainable managment of the weaner pig through nutrition

The effect of open pit mine motor transport with ICE on the air basin is examined using the example of shale mines and open pits of the production association ''Estonslanets''. Efficient areas of use of different types of open pit mine motor transport, diesel-trolley tracks and electric cars are determined based on technical-economic and ecologic feasibility.

1981-01-01

439

Environmental Research Database

DescriptionIn young pigs the post-weaning period is generally characterised by a marked reduction in voluntary feed intake, poor growth and development, and diarrhoea. The associated reduction in digestive and absorptive capacity of the gastrointestinal tract decreases the overall efficiency of nutrient capture and utilisation, thereby causing an increase in nutrient loading to the environment. The reduction in digestive and absorptive efficiency after weaning has implications for lifetime performance be [continued...

2006-01-31

440

Supplementary fired combined cycle power plants

Solar collectors with tubes partially filled with porous substrates

The present invention provides an improvement in the art of combined steam turbine and gas turbine power plants of the type wherein the principal source of energy is a fluidized bed combustor fueled by coal. More specifically, the invention pertains to supplemental firing of the gas turbine cycle with a clean hydrocarbon fuel (CHF) at a high incremental efficiency to significantly increase overall plant efficiency.

1981-03-03

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

441

Policy background - Department of Energy and Climate Change

In this work, the thermal performance of a conventional collector is improved by inserting porous substrates at the inner walls of the collector tubes. The porous substrates improve the convective heat transfer coefficient between the tube wall and the fluid. This improvement is investigated numerically and its effects on the efficiency and the useful gain of the collector are evaluated. It is found that inserting the porous substrate may raise the collector efficiency considerably, especially at high values of the overall heat loss coefficient.

1999-02-01

442

Plagiarism Detection in arXiv

...this page document analysis carried out during the policy development of the CRC Energy Efficiency Scheme. policy, background, carbon, reduction,...commitment, energy efficiency, CRC, Policy background Spending Review Announcement In the October 2010 Spending Review, the UK Government announced that the CRC ... Revenue from the sale of CRC allowances, totalling ٟ billion a year by 2014/15, will be used to support ... The implications of this announcement for CRC are: In order to clarify the price signal to participants and to support the public ...

443

PV Conversion Technologies, Session: OPV, Sensitized, Seed (Presentation)

We describe a large-scale application of methods for finding plagiarism in research document collections. The methods are applied to a collection of 284,834 documents collected by arXiv.org over a 14 year period, covering a few different research disciplines. The methodology efficiently detects a variety of problematic author behaviors, and heuristics are developed to reduce the number of false positives. The methods are also efficient enough to implement as a real-time submission screen for a collection many times larger.

2007-01-01

444

Novel procedure for an efficient and sparing cleaning procedure of turbines; Neues chemisches On-site-Reinigungsverfahren zur Wirkungsgraderhoehung von Dampfturbinen

The NREL Sensitized Solar Cell (SSC) Core Program supports the Solar America Initiative by: (1) targeting new devices and processes for commercialization by 2015 that are less expensive, more efficient, highly reliable, and environmentally benign; (2) collaborating with DOE OS/BES to conduct basic research targeting breakthroughs in key areas, such as ultra-high efficiency and/or ultra-low cost materials and devices.

2008-04-01

445

Measurement of radon emanation rates using activated charcoal

A new procedure for an efficient and sparing cleaning procedure of turbines was developed. The procedure uses cleaning and passivation chemicals that solve effectively and homogeneously salt and ferrous oxide coatings from turbines, avoiding attacks from corrosion, plugging and unbalances. In several high-pressure and condensation turbines the cleaning procedure has been already put into practice. (orig.)

2004-07-01

446

Market leadership by example: Government sector energy efficiency in developing countries

The use of charcoal canisters in the measurement of radon emanation rates from surfaces is discussed. The counting efficiency of the canisters is dependent upon the volume of activated charcoal used, varying from approximately 0.7% for a full canister to 1.3% for a canister filled to only 1/3 of its capacity. The inclusion of Si-Gel desiccant reduces the radon collection efficiency of the canister.

1983-07-01

447

MWD aids vital drilling decisions

Government facilities and services are often the largest energy users and major purchasers of energy-using equipment within a country. In developing as well as industrial countries, government ''leadership by example'' can be a powerful force to shift the market toward energy efficiency, complementing other elements of a national energy efficiency strategy. Benefits from more efficient energy management in government facilities and operations include lower government energy bills, reduced greenhouse gas emissions, less demand on electric utility systems, and in many cases reduced dependence on imported oil. Even more significantly, the government sector's buying power and example to others can generate broader demand for energy-efficient products and services, creating entry markets for domestic suppliers and stimulating competition in providing ...

2002-05-20

448

Increasing alpine transit traffic through Switzerland will considerably enhance high altitude alpine pollutant levels

Measurement-While-Drilling (MWD) sensors can supply much of the critical downhole information needed in a systems approach to improving drilling efficiency. The author looks at some areas where MWD information has helped to improve drilling efficiency. To date, most use of MWD has been for directional survey, pressure prediction, and formation evaluation. As MWD systems become more reliable and cost effective, their applications will expand.

1988-03-14

449

Highly efficient ion source for analysis of transuranium elements

Within the EU-Project VOTALP (Vertical Ozone Transports in the Alps), we have shown that deep alpine valleys like the Mesolcina Valley very efficiently transport air out of the polluted valley up to altitudes between 2000 and near 4000 m asl (above sea level). Pollutants emitted in these valleys are very efficiently transported up to high altitudes. (author) 2 figs., 1 tab., 2 refs.

1999-08-01

450

GaInP/GaAs tandem concentrator cells

Results are described of the study of the analytical applicability of a highly efficient ion source developed for a mass spectrometer. Its ionizer is in the form of a partially closed cavity with a small aperture for leading out ions, heated to a high temperature. The new ion source increases the sensitivity of the apparatus in operations with transuranium elements by almost two orders of magnitude. It is possible to perform isotopic analyses with a high salt content in the sample, and to study the characteristics of nuclear fuel, even without chemical separation of the sample elements.

1987-01-01

451

Environmental and thermal efficiency benefits by use of RDF

We discuss the initial development of a concentrator device based on the GaInP/GaAs monolithic tandem cell structure. The very high one-sun efficiency of this device, coupled with its characteristic low operating current, make this a promising candidate for use under high concentration. Test results for a prototype device are presented. This device achieves an efficiency of 29.5% at a concentration of 102 suns.

1994-06-30

452

ENERGY EFFICIENCY AND ENVIRONMENTALLY FRIENDLY DISTRIBUTED ENERGY STORAGE BATTERY

This paper presents a brief overview of refuse derived fuel (RDF) processing systems, and the different types of RDF. The quality of RDF, combustion of RDF in fluidized beds, and moving grate reactors, operating conditions, emissions (sulphur dioxide, nitrogen oxides, carbon monoxide and hydrogen chloride) and thermal efficiency are discussed. (UK).

1994-05-01

453

Drilling on the basis of reliable data. Geothermal exploration; Bohren nach verlaesslichen Planungsdaten. Geothermie

Electro Energy, Inc. conducted a research project to develop an energy efficient and environmentally friendly bipolar Ni-MH battery for distributed energy storage applications. Rechargeable batteries with long life and low cost potentially play a significant role by reducing electricity cost and pollution. A rechargeable battery functions as a reservoir for storage for electrical energy, carries energy for portable applications, or can provide peaking energy when a demand for electrical power exceeds primary generating capabilities.

2006-04-30

454

Development of an efficient, low-NOx domestic gas-range cooktop. Phase 2. Annual report, January 1984-December 1984

Geothermal heat pump systems necessitate a comprehensive approach to geology and building facilities in order to be energetically useful and efficient. Cooperation with geothermal experts should be sought at an early projecting stage in order to obtain reliable data. Geothermal data and the technical facilities of the building must be matched for the project to be both effective and efficient. (orig.)

2008-01-15

455

Development of High-Efficiency Low-Lift Vapor Compression System - Final Report

The objective of the program is to develop a domestic cooktop with low NOx emissions and higher efficiency. During the first phase of the program, a developmental prototype incorporating four IR-Jet burners was assembled. The report describes the work performed during the second phase of the program and covers optimization, and testing of the developmental prototype and design, fabrication and testing of the field-test prototype.

1985-02-01

456

Coumarin-4 laser efficiency up to 0. 14% under coaxial flash-lamp pumping

PNNL, with cofunding from the Bonneville Power Administration (BPA) and Building Technologies Program, conducted a research and development activity targeted at addressing the energy efficiency goals targeted in the BPA roadmap. PNNL investigated an integrated heating, ventilation and air conditioning (HVAC) system option referred to as the low-lift cooling system that potentially offers an increase in HVAC energy performance relative to ASHRAE Standard 90.1-2004.

2010-03-31

457

Computer Algebra meets Finite Elements: an Efficient Implementation for Maxwell's Equations

Under coaxial flash lamp pumping the laser efficiency of coumarin-4 in slightly basic ethyl alcohol solution as an active medium has reached a value of 0.14%. That is three times higher than that in the basic aqueous solution. Its tunable wavelength range of laser output has extended from 440 to 510 nm with the maximum at 460 nm.

1987-02-01

458

Aqueous liquid scintillation counting with fluor-containing nanosuspensions

We consider the numerical discretization of the time-domain Maxwell's equations with an energy-conserving discontinuous Galerkin finite element formulation. This particular formulation allows for higher order approximations of the electric and magnetic field. Special emphasis is placed on an efficient implementation which is achieved by taking advantage of recurrence properties and the tensor-product structure of the chosen shape functions. These recurrences have been derived symbolically with computer algebra methods reminiscent of the holonomic systems approach.

2011-01-01

459

VUV efficiency and radiant emittance characterization of xenon positive column discharges

A microemulsion comprised of water, Brij 78, pentanol and styrene into which PPO and bis-MSB had been dissolved was prepared. Polymerization of the styrene resulted in a suspension of fluor-containing polystyrene nanoparticles (<100 nm). After a concentration step, the aqueous nanosuspension was able to detect {sup 14}C with counting efficiencies over 50% of those of a commercially available scintillation cocktail. Monte Carlo calculations demonstrated that the size and concentration of the nanoparticles were appropriate for optimum detection efficiency.

2004-06-01

460

Use of the WNR spallation neutron source at LAMPF to determine the absolute efficiency of a neutron scintillation detector

The pure xenon dc positive column discharge has been characterized for various xenon pressures, currents, and discharge tube radii. The absolute output at the 147 nm resonance transition has been measured using two independent techniques to yield the radiant emittance at 147 nm (power per unit surface area). The efficiency of 147 nm production is obtained by combining the measured radiant emittance with the measured electrical power per unit length deposited in the gas. The radiant emittance and the efficiency are the two principle figures of merit for characterizing the discharge as a source of VUV radiation. It is observed that the trends in efficiency and radiant emittance change substantially when the discharge transitions from two-step electron impact excitation/ionization at higher pressures to single-step electron impact ionization at lower pressures. Modeling of the xenon positive column discharge yields radiant ...

1996-12-31

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Two-dimensional analysis of energy conversion efficiency for a traveling wave direct energy convertor

Prompt fission neutron spectrum measurements at the University of Massachusetts Lowell 5.5 MV Van de Graaff accelerator laboratory require that the neutron detector efficiency be well known over a neutron energy range of 100 keV to 20 MeV. The efficiency of the detector, has been determined for energies greater than 5.0 MeV using the Weapons Neutron Research (WNR) white neutron source at the Los Alamos Meson Physics Facility (LAMPF) in a pulsed beam, time-of-flight (TOF) experiment. Carbon matched polyethylene and graphite scatterers were used to obtain a hydrogen spectrum. The detector efficiency was determined using the well known H(n,n) scattering cross section. Results are compared to the detector efficiency calculation program SCINFUL available from the Radiation Shielding Information Center at Oak Ridge National Laboratory.

1994-06-01

462

Removal of thorium from simulated acid process streams by fungal biomass: potential for thorium desorption and reuse of biomass and desorbent

Two-dimensional calculations are performed to obtain the conversion efficiency of traveling wave direct energy convertor adopted in a conceptual design of field reversed configuration D-"3He fusion reactor ARTEMIS. For fusion-born 14.67MeV protons, the efficiency obtained is very small compared with that from one-dimensional analysis. This is because the effective electric field for deceleration of the proton is smaller due to distortion of potentials near girds used to excite the traveling wave and because some fractions of the incident proton beams are inevitably lost to the grids with high energy. To increase the efficiency, the potentials may have to be increased. It seems from the two-dimensional analysis performed here that the conversion efficiency obtainable is less than about 60% with the maximum potential of about 6MV, while the corresponding values from the one-dimensional analysis are about ...

463

More efficient combined energy systems. Improved efficiency of combined energy systems using fuel cells; Effizientere Verbundsysteme. Mehr Effizienz bei Energieverbundsystemen durch den Einsatz von Brennstoffzellen

When thorium was biosorbed by biomass of Rhizopus arrhizus or Aspergillus niger it could be removed by treatment using carbonate solutions, but not by phosphates or distilled water. Desorption was rapid, reaching completion in 15 and 30 min respectively. Desorption efficiency was independent of the desorbent concentration above 0.05 mol dm[sup -3] but was strongly dependent on the biomass concentration, decreasing as the biomass concentration increased for both species. Biomass from both species could be regenerated by desorption using sodium carbonate solutions and reused for biosorption. In both species, the efficiency of biosorption and desorption fell as the number of exposures increased although there was an initial rise in desorption efficiency for R. arrhizus. Biosorption and desorption efficiencies of A. niger biomass showed the steeper decline but both were severely reduced in R. arrhizus after ...

1992-01-01

464

High-efficiency solar cell and method for fabrication

Efficiency of energy coversion of primary energy to an end use in industrial countries is about 30%. This is insufficient. Evaluating on basis of exergy efficiency becomes only about 15%. Usage of fuel cells give a great chance to improve efficiency. This concerns in particular to high temperature fuel cell systems SOFC and MCFC as well as their combination with heat pumps. [German] Die Effizienz der Energieversorgung in Industrielaendern ist energetisch bewertet mit rund 30 % unbefriedigend. Legt man den strengeren, energiewirtschaftlich treffenderen exergetischen Massstab an, halbiert sich dieser Wert sogar. Brennstoffzellen bieten durch ihren Einsatz Moeglichkeiten zur Effizienzerhoehung. Dies gilt insbesondere fuer die Hochtemperatursysteme SOFC und MCFC sowie deren Verbund mit Waermepumpen.

2001-07-01

465

Energy and exergy efficiencies of ground-source heat pump systems (GSHPs) for residential applications. Paper no. IGEC-1-067

A high-efficiency 3- or 4-junction solar cell is disclosed with a theoretical AM0 energy conversion efficiency of about 40%. The solar cell includes p-n junctions formed from indium gallium arsenide nitride (InGaAsN), gallium arsenide (GaAs) and indium gallium aluminum phosphide (InGaAlP) separated by n-p tunnel junctions. An optional germanium (Ge) p-n junction can be formed in the substrate upon which the other p-n junctions are grown. The bandgap energies for each p-n junction are tailored to provide substantially equal short-circuit currents for each p-n junction, thereby eliminating current bottlenecks and improving the overall energy conversion efficiency of the solar cell. Additionally, the use of an InGaAsN p-n junction overcomes super-bandgap energy losses that are present in conventional multi-junction solar cells. A method is also disclosed for fabricating the high-efficiency 3- or 4-junction ...

1999-08-31

466

Comparison of the performance of photonic band-edge liquid crystal lasers using different dyes as the gain medium

Ground-source heat pump systems (GSHPSs), also referred to as geothermal heat pump systems (GHPSs), have been widely used in residential and commercial buildings for years due to their outstanding energy utilization efficiencies. In this study, an energy and exergy modeling of solar assisted ground-source heat pump systems for residential applications is presented for system analysis and performance evaluation. In this regard, the performance of a solar assisted ground-source heat pump heating system, installed in Solar Energy Institute of Ege University, Izmir, Turkey, is evaluated based on the actual operational data to show how energy and exergy efficiencies values change with the system. The average heating coefficient of performances (as energy efficiencies) of the solar assisted ground-source heat pump (GSHP) unit and the overall system are obtained to be 2.64 and 2.38, respectively. The average exergy ...

2005-06-12

467

Collection efficiency and decontamination factor of HEPA filter

The primary concern of this work is to study the emission characteristics of a series of chiral nematic liquid crystal lasers doped with different laser dyes (DCM, pyrromethene 580, and pyrromethene 597) at varying concentrations by weight (0.5-2 wt %) when optically pumped at 532 nm. Long-wavelength photonic band-edge laser emission is characterized in terms of threshold energy and slope efficiency. At every dye concentration investigated, the pyrromethene 597-doped lasers exhibit the highest slope efficiency (ranging from 15% to 32%) and the DCM-doped lasers the lowest (ranging from 5% to 13%). Similarly, the threshold was found to be, in general, higher for the DCM-doped laser samples in comparison to the pyrromethene-doped laser samples. These results are then compared with the spectral properties, quantum efficiencies and, where possible, fluorescence lifetimes of the dyes dispersed in a common nematic host. In ...

2010-02-01

468

A novel semidry flue gas desulfurization process with the magnetically fluidized bed reactor.

It is very important in a nuclear air cleaning system that HEPA (High Efficiency Particulate Air) filter is for reduction of releasing amounts of airborne radioactive particles. HEPA filter, by definition, has a minimum collection efficiency of 99.97 % for 0.3 #mu#m particles. However, DF (Decontamination Factor), which is necessary for safety management, can not be directly derived from the efficiency. And the current standard defined for 0.3 #mu#m particles has no scientific justification, because it has been found that the most penetrating particle size through HEPA filter is not always 0.3 #mu#m. In the present paper, a numerical experiment was made in order to estimate a relationship between DF and the efficiency. And new standard, in which the minimum DF is able to be easily obtained, was proposed. In the multistage filtration system, it was found that lower values of DF was possible to be ...

469

Follicle-stimulating hormone receptor-mediated uptake of "4"5Ca"2"+ by cultured rat Sertoli cells does not require activation of cholera toxin- or pertussis toxin-sensitive guanine nucleotide binding proteins or adenylate cyclase

The magnetically fluidized bed (MFB) was used as the reactor in a novel semidry flue gas desulfurization (FGD) process to achieve high desulfurization efficiency. Experiments in a laboratory-scale apparatus were conducted to reveal the effects of approach to adiabatic saturation temperature, Ca/S molar ratio and applied magnetic field intensity on SO(2) removal. Results showed that SO(2) removal efficiency can be obviously enhanced by decreasing approach to adiabatic saturation temperature, increasing Ca/S molar ratio, or increasing applied magnetic field intensity. At a magnetic field intensity of 300Oe and a Ca/S molar ratio of 1.0, the desulfurization efficiency (excluding desulfurization efficiency in the fabric filter) was over 80%, while spent sorbent appeared in the form of dry powder. With the SEM, XRD and EDX research, it can be found that the increase of DC magnetic field intensity can make ...

2009-03-18

470

Strategic Energy Planning (Area 1) Consultants Reports to Citizen Potawatomi Nation Federally Recognized Indian Tribe

We have previously reported that FSH stimulates flux of 45Ca2+ into cultured Sertoli cells from immature rats via voltage-sensitive and voltage-independent calcium channels. In the present study, we show that this effect of FSH does not require cholera toxin (CT)- or pertussis toxin (PT)-sensitive guanine nucleotide binding (G) protein or activation of adenylate cyclase (AC). Significant stimulation of 45Ca2+ influx was observed within 1 min, and maximal response (3.2-fold over basal levels) was achieved within 2 min after exposure to FSH. FSH-stimulated elevations in cellular cAMP paralleled increases in 45Ca2+ uptake, suggesting a possible coupling of AC activation to 45Ca2+ influx. (Bu)2cAMP, however, was not able to enhance 45Ca2+ uptake over basal levels at a final concentration of 1000 microM, although a concentration-related increase in androstenedione conversion to estradiol was evident. Exposure of Sertoli cells to CT (10 ng/ml) consistently stimulated basal levels of ...

1990-01-01

471

Theoretical analysis of the DC electromagnetic flow coupler

The assets that Citizen Potawatomi Nation holds were evaluated to help define the strengths and weaknesses to be used in pursuing economic prosperity. With this baseline assessment, a Planning Team will create a vision for the tribe to integrate into long-term energy and business strategies. Identification of energy efficiency devices, systems and technologies was made, and an estimation of cost benefits of the more promising ideas is submitted for possible inclusion into the final energy plan. Multiple energy resources and sources were identified and their attributes were assessed to determine the appropriateness of each. Methods of saving energy were evaluated and reported on and potential revenue-generating sources that specifically fit the tribe were identified and reported. A primary goal is to create long-term energy strategies to explore development of tribal utility options and analyze renewable energy and energy efficiency options. ...

2004-12-01

472

The efficiency of coal-fired combined-cycle powerplants

A descriptive model and design procedure for the DC electromagnetic flow coupler is developed based on a quasi-one-dimensional analysis previously developed for the DC electromagnetic pump. It is shown that for a particular flow coupler geometry, the total efficiency and the pressure gradients through the pump and generator depend on two parameters - the Hartmann number and the ratio of the pump flow rate to generator flow rate. Thus, for a fixed Hartmann number the efficiency depends only on the flow ratio. However, for a fixed pressure rise through the pump it is shown that the efficiency depends only on the Hartmann number. Nomographs showing the operating characteristics and critical design points are presented. Example calculations for a full-size unit, suitable for use in a liquid-metal cooled fast breeder reactor, are also discussed using the design nomographs.

1983-03-01

473

Removal of NO and SO2 in Corona Discharge Plasma Reactor with Water Film

Concepts involving combined gas-turbine/steam-turbine power-generation plants, in which the fuel gas for the gas turbine is produced via the gasification of coal, are now extremely advanced. This technology already permits efficiencies of around 50% current development targets view 65% as achievable. In conventional technology, efficiencies are tied to conditions, such as air and cooling-water temperatures, at the particular location. In combined-cycle power plants, the properties of the fuel coal also play an important part. There are, in face, coals which can be more advantageously used in a combined-cycle power plant than in a conventional one. These differences, combined with advantageous concepts for coal-fired combined-cycle power-generating plants, are presented and analyzed. Particular attention is devoted to individual losses occurring at coal conversion, thermodynamic cycles, integration of processes and internal consumptions. 14 ...

1996-12-01

474

Reducing barriers to energy efficiency in the German higher education sector. Final report

In this paper, a novel type of a corona discharge plasma reactor was designed, which consists of needle-plate-combined electrodes, in which a series of needle electrodes are placed in a glass container filled with flue gas, and a plate electrode is immersed in the water. Based on this model, the removal of NO and SO2 was tested experimentally. In addition, the effect of streamer polarity on the reduction of SO2 and NO was investigated in detail. The experimental results show that the corona wind formed between the high-voltage needle electrode and the water by corona discharge enhances the cleaning efficiency of the flue gas because of the presence of water, and the cleaning efficiency will increase with the increase of applied dc voltage within a definite range. The removal efficiency of SO2 up to 98%, and about 85% of NOx removal under suitable conditions is obtained in our experiments.

2004-04-01

475

Radioactivity decontamination efficiency of ceramic filter in an incineration volume reduction system of radioactive waste

This report describes the empirical research into barriers to energy efficiency in the German higher education (HE) sector. It is one of nine such reports in the BARRIERS project. The report contains description and analysis of six case studies of energy management in German universities. The results are analysed using the theoretical framework developed for the BARRIERS project (Sorrell et al., 2000). The report also provides brief recommendations on how these barriers to the rational use of energy (RUE) may be overcome and how energy efficiency within the sector may be improved. The results of the study for the higher education sector in Germany are summarised in this executive summary under the following headings: - Characterising the higher education sector; - Case studies of energy management in the German higher education sector; - Evidence of barriers in the German higher education sector; - The role of energy service companies in the ...

2000-12-01

476

Radioactivity decontamination efficiency of ceramic filter in an incineration volume reduction system of radioactive waste

The small pilot facility of a cyclone type suspension incineration system of radioactive waste was set up in order to evaluate the decontamination efficiency of a high efficiency ceramic filter. The evaluation was made by use of /sup 54/Mn, /sup 59/Fe, /sup 60/Co, /sup 65/Zn and /sup 137/Cs. 1. The decontamination factor by one line of ceramic filter for every species were over 10/sup 5/. 2. The decontamination factor increased by one oder when water vapor exists in off-gas. The same tendency was also observed when iron dioxide existed at the incineration of cation exchange resin.

1987-02-01

477

Office of Industrial Technologies research in progress

The small pilot facility of a cyclone type suspension incineration system of radioactive waste was set up in order to evaluate the decontamination efficiency of a high efficiency ceramic filter. The evaluation was made by use of "5"4Mn, "5"9Fe, "6"0Co, "6"5Zn and "1"3"7Cs. 1. The decontamination factor by one line of ceramic filter for every species were over 10"5. 2. The decontamination factor increased by one oder when water vapor exists in off-gas. The same tendency was also observed when iron dioxide existed at the incineration of cation exchange resin. (author).

478

Improved recovery demonstration for Williston basin carbonates. Quarterly technical progress report, October 1, 1995--December 31, 1995

The US Department of Energy (DOE) Office of Industrial Technologies (OIT) conducts research and development activities which focus on improving energy efficiency and providing for fuel flexibility within US industry in the area of industrial conservation. The mission of OIT is to increase the utilization of existing energy-efficient equipment and to find and promote new, cost-effective ways for industrial facilities to improve their energy efficiency and minimize waste products. To ensure advancement of the technological leadership of the United States and to improve the competitiveness of American industrial products in world markets, OIT works closely with industrial partners, the staffs of the national laboratories, and universities to identify research and development needs and to solve technological challenges. This report contains summaries of the currently active projects supported by the Office of Industrial ...

1993-05-01

479

Improved recovery demonstration for Williston basin carbonates. Annual report, June 10, 1994--June 9, 1995

The purpose of this project is to demonstrate targeted infill and extension drilling opportunities, better determinations of oil-in-place, methods for improved completion efficiency and the suitability of waterflooding in certain shallow-shelf carbonate reservoirs in the Williston Basin, Montana, North Dakota and South Dakota. Improved reservoir characterization utilizing 3-dimensional and multi-component seismic area is being investigated for identification of structural and stratigraphic reservoir compartments. These seismic characterization tools are integrated with geological and engineering studies. Improved completion efficiency is being tested with extended-reach jetting lance and other ultra-short radius lateral technologies. Improved completion efficiency, additional wells at closer spacing and better estimates of oil-in-place will result in additional oil production by primary and enhanced recovery processes.

1996-02-01

480

Improved recovery demonstration for Williston Basin carbonates. Quarterly technical progress report, October--December 1996

The purpose of this project is to demonstrate targeted infill and extension drilling opportunities, better determinations of oil-in-place, methods for improved completion efficiency and the suitability of waterflooding in Red River and Ratcliffe shallow-shelf carbonate reservoirs in the Williston Basin, Montana, North Dakota and South Dakota. Improved reservoir characterization utilizing three-dimensional and multi-component seismic are being investigated for identification of structural and stratigraphic reservoir compartments. These seismic characterization tools are integrated with geological and engineering studies. Improved completion efficiency is being tested with extended-reach jetting lance and other ultra-short-radius lateral technologies. Improved completion efficiency, additional wells at closer spacing and better estimates of oil in place will result in additional oil recovery by primary and enhanced recovery ...

1995-09-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

481

Improved recovery demonstration for Williston Basin carbonates. Annual report, June 10, 1995--June 9, 1996

The purpose of this project is to demonstrate targeted infill and extension drilling opportunities, better determinations of oil-in-place, methods for improved completion efficiency and the suitability of waterflooding in certain shallow-shelf carbonate reservoirs in the Williston Basin, Montana, North Dakota and South Dakota. Improved reservoir characterization utilizing 3-dimensional (3D) and multi-component seismic are being investigated for identification of structural and stratigraphic reservoir compartments. These seismic characterization tools are integrated with geological and engineering studies. Improved completion efficiency is being tested with short-lateral and horizontal drilling technologies. Improved completion efficiency, additional wells at closer spacing and better estimates of oil-in-place will result in additional oil production by primary and enhanced recovery processes.

1997-04-01

482

Improved recovery demonstration for Williston Basin carbonates

The purpose of this project is to demonstrate targeted infill and extension drilling opportunities, better determinations of oil-in-place, methods for improved completion efficiency and the suitability of waterflooding in Red River and Ratcliffe shallow-shelf carbonate reservoirs in the Williston Basin, Montana, North Dakota and South Dakota. Improved reservoir characterization utilizing three-dimensional and multi-component seismic are being investigated for identification of structural and stratigraphic reservoir compartments. These seismic characterization tools are integrated with geological and engineering studies. Improved completion efficiency is being tested with extended-reach jetting lance and other ultra-short-radius lateral technologies. Improved completion efficiency, additional wells at closer spacing and better estimates of oil in place will result in additional oil recovery by primary and enhanced recovery ...

1996-09-01

483

Improved recovery demonstration for Williston Basin carbonates

The purpose of this project is to demonstrate targeted infill and extension drilling opportunities, better determinations of oil-in- place, methods for improved completion efficiency and the suitability of waterflooding in certain shallow-shelf carbonate reservoirs in the Williston Basin, Montana, North Dakota and South Dakota. Improved reservoir characterization utilizing 3-dimensional (3D) and multi- component seismic are being investigated for identification of structural and stratigraphic reservoir compartments. These seismic characterization tools are integrated with geological and engineering studies. Improved completion efficiency is being tested with short- lateral and horizontal drilling technologies. Improved completion efficiency, additional wells at closer spacing and better estimates of oil-in-place will result in additional oil production by primary and enhanced recovery processes.

1996-09-01

484

Improved Recovery Demonstration for Williston Basin Carbonates.

The purpose of this project is to demonstrate targeted infill and extension drilling opportunities, better determination of oil-in-place, methods for improved completion efficiency and the suitability of waterflooding in certain shallow-shelf carbonate reservoirs in the Williston Basin, Montana, North Dakota and South Dakota. Improved reservoir characterization utilizing 3-dimensional (3D) is being investigated for identification of structural and stratigraphic reservoir compartments. These seismic characterization tools are integrated with geological and engineering studies. Improved completion efficiency is being tested with short lateral and horizontal drilling technologies. Improved completion efficiency, additional wells at closer spacing and better estimates of oil-in- place will result in additional oil production by primary and enhanced recovery processes.

1997-12-01

485

Improved Recovery Demonstration for Williston Basin Carbonates

The purpose of this project is to demonstrate targeted infill and extension drilling opportunities, better determinations of oil-in- place, methods for improved completion efficiency and the suitability of waterflooding in certain shallow-shelf carbonate reservoirs in the Williston Basin, Montana, North Dakota and South Dakota. Improved reservoir characterization utilizing 3-dimensional (3-D) and multi-component seismic are being investigated for identification of structural and stratigraphic reservoir compartments. These seismic characterization tools are integrated with geological and engineering studies. Improved completion efficiency is being tested with short-lateral and horizontal drilling technologies. Improved completion efficiency, additional wells at closer spacing and better estimate of oil-in-place will result in additional oil production by primary and enhanced recovery processes.

1997-12-31

486

Improved Recovery Demonstration for Williston Basin Carbonates

The purpose of this project is to demonstrate targeted infill and extension drilling opportunities, better determinations of oil-in- place, methods for improved completion efficiency and the suitability of waterflooding in certain shallow-shelf carbonate reservoirs in the Williston Basin, Montana, North Dakota and South Dakota. Improved reservoir characterization utilizing 3-dimensional (3-D) and multi- component seismic are being investigated for identification of structural and stratigraphic reservoir compartments. These seismic characterization tools are integrated with geological and engineering studies. Improved completion efficiency is being tested with short- lateral and horizontal drilling technologies. Improved completion efficiency, additional wells at closer spacing and better estimate of oil-in-place will result in additional oil production by primary and enhanced recovery processes.

1997-03-01

487

Gas turbines aim at world power market dominance

The purpose of this project is to demonstrate targeted infill and extension drilling opportunities, better determinations of oil-in-place, methods for improved completion efficiency and the suitability of waterflooding in certain shallow-shelf carbonate reservoirs in the Williston Basin, Montana, North Dakota and South Dakota. Improved reservoir characterization utilizing three-dimensional is being investigated for identification of structural and stratigraphic reservoir compartments. These seismic characterization tools are integrated with geological and engineering studies. Improved completion efficiency is being tested with short-lateral and horizontal drilling technologies. Improved completion efficiency, additional wells at closer spacing and better estimates of oil-in-place will result in additional oil production by primary and enhanced recovery processes.

1997-12-31

488

Gas turbine tops 40% efficiency; features novel inlet-air cooler

Rapid technology improvements, resulting in high efficiency, emissions reduction and low generation cost, are making gas turbines the generation technology of choice despite some recent reliability problems. The basic reason for the dominance is high efficiency. The rapid pace of gas-turbine technology improvement in the 1990s drove combined-cycle thermal efficiency to nearly 60 percent with natural gas as the fuel. It will probably go even higher after the year 2000. In addition, the gas-fired combined cycle is a bargain. With natural gas prices where they are right now, it is the least-cost generation operation for power producers with access to gas. Many will replace older, high-cost power plants with new gas-fired combined cycle power plants.

1996-06-01

489

From waste prevention to promotion of material efficiency: change of discourse in the waste policy of Finland

This article reports on the world's first gas turbine to top 40% thermal efficiency in simple cycle and is now operating commercially as part of a natural-gas-fired combined-cycle cogeneration (CC) plant serving the Ottawa Health Sciences Center, Ottawa, Ont, Canada. Owned and operated by TransAlta Energy Corp, Calgary, Alta, the CC plant supplies steam and hot and chilled water to the health center and another thermal user while the electricity is sold to Ontario Hydro. Part of the project included the conversion of 15-psig steam-carrying equipment to hot-water service. Commercial success of this installation heralds a new era in the application of efficient gas turbines for power generation.

1993-02-01

490

British Library Electronic Table of Contents (United Kingdom)

This article analyses the process of preparing the proposal for a new Finnish National Waste Plan (NWP 2007-2016). The focus of this study is on the use of the alternative concepts of waste prevention or material efficiency and on the shift in discourse from the former to the latter concept. The strengths and weaknesses of these competing concepts were analysed using criteria such as synergy, semantic aspects, legal context and applicability to monitoring. The discourse presented by different stakeholder groups was analysed. The implications of choosing either of the concepts were illustrated. The author concludes that waste prevention can be promoted just as well, or even better from the perspective of improving material efficiency. The concept must be complemented by policy instruments w...

2009-01-01

491

First and second law analysis of diesel engine powered cogeneration systems

First and second law analysis of diesel engine powered cogeneration systems

In this article, the thermodynamic analysis of the existing diesel engine cogeneration system is performed. All necessary data are obtained from the actual diesel engine cogeneration plant located at Gaziantep, Turkey. The exergy analysis is aimed to evaluate the exergy destruction in each component as well as the exergetic efficiencies. The thermodynamic performance of a 25.32 MW electricity and 8.1 tons/h steam capacity diesel engine cogeneration system at full load conditions is analyzed. The thermal efficiency of the overall plant is found to be 44.2% and the exergetic efficiency is 40.7%. The exergy balance equations developed in this paper may also be utilized in the exergoeconomic analysis to estimate the production costs depending on various input costs in a diesel cogeneration system.

2008-08-01

492

British Library Electronic Table of Contents (United Kingdom)

In this article, the thermodynamic analysis of the existing diesel engine cogeneration system is performed. All necessary data are obtained from the actual diesel engine cogeneration plant located at Gaziantep, Turkey. The exergy analysis is aimed to evaluate the exergy destruction in each component as well as the exergetic efficiencies. The thermodynamic performance of a 25.32MW electricity and 8.1tons/h steam capacity diesel engine cogeneration system at full load conditions is analyzed. The thermal efficiency of the overall plant is found to be 44.2% and the exergetic efficiency is 40.7%. The exergy balance equations developed in this paper may also be utilized in the exergoeconomic analysis to estimate the production costs depending on various input costs in a diesel cogeneration syste...

2008-01-01

493

Engine use of producer gas, experiences and requirements

Electricity demand for South Korean residential sector

The most effective way to generate electricity out of biomass is the gasification and the use of the gas in a gas engine. The conversion of the organic carbon with the gasification of biomass is higher than 95 %. Depending on the gasification concept, the efficiency of gasifiers is found between 70 and 90 %. If the pyrolysis gas is used in a gas engine, an electric efficiency of about 26 % referring to the primary amount of energy can be obtained. With efficient gas cleaning (cleaning for tar and dust), pyrolysis gas is well qualified for engine combustion. Through modern state of engine controlling there are ways to have complete control of the fluctuations in the gas composition. Furthermore, the minor calorific value of offered wood gas poses no problem for turbo charged lean-burn gas engines. (orig.)

1999-07-01

494

Electricity demand for South Korean residential sector

This study estimates the electricity demand function for the residential sector of South Korea with the aim of examining the effects of improved energy efficiency, structural factors and household lifestyles on electricity consumption. In the study, time series data for the period from 1973 to 2007 is used in a structural time series model to estimate the long-term price and income elasticities and annual growth of underlying energy demand trend (UEDT) at the end of the estimation period. The result shows a long-term income elasticity of 1.33 and a long-term price elasticity of -0.27% with -0.93% as the percentage growth of UEDT at the end of the estimation period. This result suggests that, in order to encourage energy efficiency in the residential sector, the government should complement the market based pricing policies with non-market policies such as minimum energy efficiency standards and public enlightenment.

2009-12-15

495

Coordinated synthesis of multimachine power system stabilizer using an efficient decentralized modal control (DMC) algorithm

This study estimates the electricity demand function for the residential sector of South Korea with the aim of examining the effects of improved energy efficiency, structural factors and household lifestyles on electricity consumption. In the study, time series data for the period from 1973 to 2007 is used in a structural time series model to estimate the long-term price and income elasticities and annual growth of underlying energy demand trend (UEDT) at the end of the estimation period. The result shows a long-term income elasticity of 1.33 and a long-term price elasticity of -0.27% with -0.93% as the percentage growth of UEDT at the end of the estimation period. This result suggests that, in order to encourage energy efficiency in the residential sector, the government should complement the market based pricing policies with non-market policies such as minimum energy efficiency standards and public enlightenment. ...

2009-12-15

496

Comparative Evaluation of Different Cell Lysis and Extraction Methods for Studying Benzo(a)pyrene Metabolism in HT-29 Colon Cancer Cell Cultures

This paper presents a new method for simultaneously selecting the power system stabilizer (PSS) parameters in multimachine power systems. Design of local output feedback power system stabilizer using local signal of the generator (e.g. speed or power) is formulated as a decentralized modal control (DMC) problem. Exact model reduction based on modal control theory is proposed to make the determination of PSS parameters highly efficient. Results obtained from the coordinated tuning of the parameters of three power system stabilizers equipped on a multimachine power system show that exact assignment of the eigenvalues associated with the poorly damped electromechanical modes can be achieved in a very efficient manner.

1986-01-01

497

British Library Electronic Table of Contents (United Kingdom)

Abstract Lysis and extraction of cells are essential sample processing steps for investigations pertaining to metabolism of xenobiotics in cell culture studies. Of particular importance to these procedures are maintaining high lysis efficiency and analyte integrity as they influence the qualitative and quantitative distribution of drug and toxicant metabolites in the intra- and extracellular milieus. In this study we have compared the efficiency of different procedures viz. homogenization, sonication, bead beating, and molecular grinding resin treatment for disruption of HT-29 colon cells exposed to benzo(a)pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH) compound and a suspected colon carcinogen. Also, we have evaluated the efficiency of various procedures for extracting BaP parent c...

2011-01-01

498

An efficient algorithm for the design of decentralized output feedback power system stabilizer

A Kuznets curve in environmental efficiency. An application on OECD countries

A new algorithm for the design of decentralized output feedback stabilizers for large-scale electric power systems is presented in this paper. In the proposed approach, the generators which are most effective for stabilizer applications are first identified by using participation factors. Then an efficient algorithm based on decentralized pole assignment is proposed for the determination of the parameters of the power system stabilizers which, due to the difficulty associated with the communication among the geographically dispersed generating stations in a large power system, are essentially decentralized compensators using local generator outputs as their feedback signals. The proposed method is computationally efficient and can be applied to any large-scale system. The simplicity and effectiveness of the proposed method are demonstrated by an example of stabilizer design for a practical power system.

1988-08-01

499