yeast alters meiotic: Topics by WorldWideScience.org

Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli

BackgroundThe production of high yields of recombinant proteins is an enduring bottleneck in the post-genomic sciences that has yet to be addressed in a truly rational manner. Typically...Full Text Available

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Survey and analysis of simple sequence repeats in the Laccaria bicolor genome, with development of microsatellite markers

BackgroundThe large sensitivity, high reproducibility and essentially unlimited dynamic range of real-time PCR to measure gene expression in complex samples provides the opportunity...Full Text Available

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Use of polarized light microscopy in porcine reproductive technologies

It is becoming clear that simple sequence repeats (SSRs) play a significant role in fungal genome organization, and they are a large source of genetic markers for population genetics and meiotic maps. We identified SSRs in the Laccaria bicolor genome by in silico survey and analyzed their distribution in the different genomic regions. We also compared the abundance and distribution of SSRs in L. bicolor with those of the following fungal genomes: Phanerochaete chrysosporium, Coprinopsis cinerea, Ustilago maydis, Cryptococcus neoformans, Aspergillus nidulans, Magnaporthe grisea, Neurospora crassa and Saccharomyces cerevisiae. Using the MISA computer program, we detected 277,062 SSRs in the L. bicolor genome representing 8% of the assembled genomic sequence. Among the analyzed basidiomycetes, L. bicolor exhibited the highest SSR density although no correlation between relative abundance and the genome sizes was observed. In most genomes the short motifs (mono- to ...

2011-01-01

5

British Library Electronic Table of Contents (United Kingdom)

The meiotic spindle in the oocyte is composed of microtubules and plays an important role during chromosome alignment and separation at meiosis. Polarized light microscopy (PLM) could be useful for a non-invasive evaluation of the meiotic spindle and may allow removal of nuclear structures without fluorochrome staining and ultraviolet exposure. In this study, PLM was used to assess its potential application in porcine reproductive technologies. The objectives of the present study were to assess the efficiency of PLM to detect microtubule-polymerized protein in in vitro-matured porcine oocytes; to examine its effects on the oocyte developmental competence; to select oocytes based on the presence of the meiotic spindle detected by PLM; and to assess the efficiency oocyte enucleation assisted...

2011-01-01

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Misregulation of the Kinesin-like Protein Subito Induces Meiotic Spindle Formation in the Absence of Chromosomes and Centrosomes

Meiotic recombination in the beta globin gene cluster causing an error in prenatal diagnosis of beta thalassaemia.

Bipolar spindles assemble in the absence of centrosomes in the oocytes of many species. In Drosophila melanogaster oocytes, the chromosomes have been proposed to initiate spindle assembly...Full Text Available

2007-09-01

7

Reactive biomolecular divergence in genetically altered yeast cells and isolated mitochondria as measured by biocavity laser spectroscopy : a rapid diagnostic method for studying cellular responses to stress and disease.

In the course of a prenatal diagnosis for beta thalassaemia by linkage analysis of restriction fragment length polymorphisms, a homozygous beta thalassaemia fetus was misdiagnosed as beta thalassaemia...Full Text Available

1988-05-01

8

Survival of Genetically Modified and Self-Cloned Strains of Commercial Baker's Yeast in Simulated Natural Environments: Environmental Risk Assessment

We report an analysis of four strains of baker's yeast (Saccharomyces cerevisiae) using biocavity laser spectroscopy. The four strains are grouped in two pairs (wild type and altered), in which one strain differs genetically at a single locus, affecting mitochondrial function. In one pair, the wild-type rho+ and a rho0 strain differ by complete removal of mitochondrial DNA (mtDNA). In the second pair, the wild-type rho+ and a rho- strain differ by knock-out of the nuclear gene encoding Cox4, an essential subunit of cytochrome c oxidase. The biocavity laser is used to measure the biophysical optic parameter Deltalambda, a laser wavelength shift relating to the optical density of cell or mitochondria that uniquely reflects its size and biomolecular composition. As such, Deltalambda is a powerful parameter that rapidly interrogates the biomolecular state of single cells and mitochondria. Wild-type cells and mitochondria produce ...

2006-12-01

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Comparison of the Survival and Metabolic Activity of Psychrophilic and Mesophilic Yeasts Subjected to Freeze-Thaw Stress

Although genetic engineering techniques for baker's yeast might improve the yeast's fermentation characteristics, the lack of scientific data on the survival of such strains in natural environments...Full Text Available

2005-11-01

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Global Transcriptional Responses of Fission Yeast to Environmental Stress

A mesophilic yeast, Candida utilis, and a psychrophilic yeast, Leucosporidium stokesii, were subjected to freeze-thaw cycling over the range 25 to -60 C. Viability...Full Text Available

1975-06-01

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Apoptosis at Inflection Point in Liquid Culture of Budding Yeasts

We explored transcriptional responses of the fission yeast Schizosaccharomyces pombe to various environmental stresses. DNA microarrays were used to characterize changes in expression...Full Text Available

2003-01-01

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Promoter Structure Which Affects on the Expression of Yeast MGMT Gene

Budding yeasts are highly suitable for aging studies, because the number of bud scars (stage) proportionally correlates with age. Its maximum stages are known ...Full Text Available

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Effect of Zinc-Enriched Yeast Supplementation on Serum Zinc and Testosterone Concentrations in Ethanol Feeding Rats

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1997-01-01

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Application of Quantitative Real-Time PCR for Enumeration of Total Bacterial, Archaeal, and Yeast Populations in Kimchi

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2008-07-01

15

Altering Sexual Development in Arabidopsis

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2009-12-01

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Small heat shock protein LimHSP16.45 protects pollen mother cells and tapetal cells against extreme temperatures during late zygotene to pachytene stages of meiotic prophase I in David Lily

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1998-06-01

17

British Library Electronic Table of Contents (United Kingdom)

Plant meiotic prophase I is a complicated process involving the late zygotene and pachytene stages, both crucial for completing synapsis and recombination. Using David Lily (Lilium davidii var. Willmottiae) as our research material, we performed suppression subtractive hybridization to construct EST library of anthers at various stages of development by the pollen mother cells. From this library, we identified small heat shock protein LimHSP16.45 was highly expressed during the late zygotene to pachytene stages. Our results also showed that LimHSP16.45 was almost specifically expressed in the anther compared with the root, stem, or leaf, and in situ expression of LimHSP16.45 mRNAs showed strong signals in the pollen mother cells and tapetal cells. LimHSP16.45 could be induced by heat and c...

2011-01-01

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Modular coherence of protein dynamics in yeast cell polarity system

METABOLIC REGULATION OF ADENOSINE TRIPHOSPHATE SULFURYLASE IN YEAST

In this study, we investigated on a systems level how complex protein interactions underlying cell polarity in yeast determine the dynamic association of proteins with the polar cortical domain (PCD)...Full Text Available

2011-05-03

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In vivo expression and mitochondrial targeting of yeast apoiso-1-cytochrome c fusion proteins.

de Vito, Peter C. (Princeton University, Princeton, N.J.), and Jacques Dreyfuss. Metabolic regulation of adenosine triphosphate sulfurylase in yeast. J. Bacteriol. 88:1341–1348....Full Text Available

1964-11-01

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Evaluation of Acyl Coenzyme A Oxidase (Aox) Isozyme Function in the n-Alkane-Assimilating Yeast Yarrowia lipolytica

To define the import pathway for apoiso-1-cytochrome c in vivo, the coding region for bacterial chloramphenicol acetyltransferase (CAT) or yeast copper metallothionein (CuMT) was fused to the carboxy...Full Text Available

1990-11-01

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Antagonistic Gcn5-Hda1 interactions revealed by mutations to the Anaphase Promoting Complex in yeast

We have identified five acyl coenzyme A (CoA) oxidase isozymes (Aox1 through Aox5) in the n-alkane-assimilating yeast Yarrowia lipolytica, encoded by the POX1...Full Text Available

1999-09-01

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A Novel Function of the DNA Repair Gene rhp6 in Mating-Type Silencing by Chromatin Remodeling in Fission Yeast

BackgroundHistone post-translational modifications are critical for gene expression and cell viability. A broad spectrum of histone lysine residues have been identified in yeast...Full Text Available

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DNA double strand breakage after UV irradiation of yeast cells; DNA-Doppelstrangbrueche nach UV-Bestrahlung in Hefezellen

Recent studies have indicated that the DNA replication machinery is coupled to silencing of mating-type loci in the budding yeast Saccharomyces cerevisiae, and a similar silencing mechanism...Full Text Available

1998-09-01

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Cholera toxin binding sites in yeast triggers biochemical pathway

No abstract prepared.

2000-07-01

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Characterization of commercially available selenized yeast food supplements

... e Biologia Molecular (SBBq), Sao Paulo, SP (Brazil) 217 p. APPLIED LIFE

1998-05-23

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Effect of UV radiation on the killer phenotype in the wine yeast-saccharomycetes and spontaneous variation of this character

... Rome (Italy) Istituto Superiore di Sanita (Italy) 207 p. ENVIRONMENTAL

2009-04-01

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Removal of lead from solution using non-living residual brewery yeast

Spontaneous and ultraviolet-induced changeabilities of wine yeasts from the killer state to sensitive one have been studied. Observed often spontaneous changes of killer and neutral phenotypes under laboratory store conditions as well as high mutation frequency of genetic elements responsible for the killer indication on ultraviolet irradiation testify that often encounterability in nature and in the production of sensitive yeasts is attributed to high frequency of mutation changes of the killer and neutral phenotypes to the sensitive state.

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Genetical approach to oxygen toxicity. [Saccharomyces cerevisiae

A number of preparations of residual non-living brewery yeast were examined for their ability to remove lead from solution. Those preparations included washed and un-washed intact yeast and washed and un-washed homogenates of the yeast cells. Using biosorption isotherm analysis it was found that the washed and un-washed preparations of intact, non-living yeast exhibited maximum biosorption capacities for lead of 127 and 99 mg/g dry weight biomass, respectively. The washed and un-washed cell homogenates exhibited maximum biosorption capacities of 38 and 139 mg lead/g dry weight biomass, respectively. Since it had previously been shown that these preparations of biomass were capable of removing uranium from solution by combined biosorption and precipitation processes, it was decided to examine removal of lead from solution using a form of equilibrium dialysis in which the biomass was retained within a ...

1998-10-01

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Change management altering mindsets in a global context

The role of intermediate products of dioxygen reduction in cytotoxic effects ascribed to oxygen molecules was studied in vivo using various yeast mutants with changed response to oxygen stress. It has been documented that superoxide radical exerts its deleterious effects on yeast cells directly and the role of other oxygen species derived from it is hardly detectable. Yeast Saccharomyces cerevisiae, however, cannot be considered as a typical eukaryotic organism due to its inability of synthesizing polyunsaturated fatty acids (pufa). These fatty acids are known as main target molecules during oxidative stress and their peroxidation leads to cytotoxic effects. As fatty acid content could be easily manipulated in yeast, this organism was used to evaluate the contribution of pufa peroxidation process to the cytotoxic effects of oxygen. Results obtained show, that yeast cells containing ...

1986-01-01

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All diesel exhaust is not the same: engine load alters toxicity.

Change management

2006-01-01

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Transformation by complementation of an adenine auxotroph of the lignin-degrading basidiomycete Phanerochaete chrysosporium

PMID:21807581

2011-08-01

32

Sirtuins, Bioageing, and Cancer

Swollen basiodiospores of an adenine auxotroph of Phanerochaete chrysosporium were protoplasted with Novozyme 234 and transformed to prototrophy by using a plasmid containing the gene for an adenine biosynthetic enzyme from Schizophyllum commune. Transformation frequencies of 100 transformants per {mu}g of DNA were obtained. Southern blot analysis of DNA extracted from transformants demonstrated that plasmid DNA was integrated into the chromosomal DNA in multiple tandem copies. Analysis of conidia and basiodiospores from transformants demonstrated that the transforming character was mitotically and meiotically stable on both selective and nonselective media. Genetic crosses between double mutants transformed for adenine prototrophy and other auxotrophic strains yielded Ade{sup {minus}} progeny, which indicated that integration occurred at a site(s) other than the resident adenine biosynthetic gene.

1989-02-01

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Iodination of glyceraldehyde 3-phosphate dehydrogenase

The Sirtuins are a family of orthologues of yeast Sir2 found in a wide range of organisms from bacteria to man. They display a high degree of conservation between species, in both sequence and function,...Full Text Available

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Effect of Photosynthetic Bacterial Addition to Chlorella or ${\\omega}-Yeast$ on Growth of Rotifer, Brachionus plicatilis, and its Dietary Value for Flounder, Paralichthys olivaceus, Larvae

1. A high degree of homology in the positions of tyrosine residues in glyceraldehyde 3-phosphate dehydrogenase from lobster and pig muscle, and from yeast, prompted an examination of the reactivity...Full Text Available

1970-09-01

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The Functionally Conserved Nucleoporins Nup124p from Fission Yeast and the Human Nup153 Mediate Nuclear Import and Activity of the Tf1 Retrotransposon and HIV-1 VprV?

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2000-03-01

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Morphological Instabilities in a growing Yeast Colony Experiment and Theory

We report that the fission yeast nucleoporin Nup124p is required for the nuclear import of both, retrotransposon Tf1-Gag as well as the retroviral HIV-1 Vpr. Failure to import Tf1-Gag into the nucleus...Full Text Available

2005-04-01

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Kes1p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis.

We study the growth of colonies of the yeast Pichia membranaefaciens on agarose film. The growth conditions are controlled in a setup where nutrients are supplied through an agarose film suspended over a solution of nutrients. As the thickness of the agarose film is varied, the morphology of the front of the colony changes. The growth of the front is modeled by coupling it to a diffusive field of inhibitory metabolites. Qualitative agreement with experiments suggests that such a coupling is responsible for the observed instability of the front.

1997-01-01

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Dissecting toxin immunity in virus-infected killer yeast uncovers an intrinsic strategy of self-protection

The yeast phosphatidylinositol transfer protein (Sec14p) is required for biogenesis of Golgi-derived transport vesicles and cell viability, and this essential Sec14p requirement is abrogated by inactivation...Full Text Available

1996-12-02

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Toxin-secreting “killer” yeasts were initially identified >40 years ago in Saccharomyces cerevisiae strains infected with a double-stranded RNA “killer”...Full Text Available

2006-03-07

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DNA repair genes

Arginine aminoacylation identity is context-dependent and ensured by alternate recognition sets in the anticodon loop of accepting tRNA transcripts.

Fission yeast S. pombe is assumed to be a good model for cloning of human DNA repair genes, because human gene is normally expressed in S. pombe and has a very similar protein sequence to yeast protein. We have tried to elucidate the DNA repair mechanisms of S. pombe as a model system for those of mammals. (J.P.N.)

1995-12-01

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Amino acid sequences that determine the nuclear localization of yeast histone 2B.

Yeast arginyl-tRNA synthetase recognizes the non-modified wild-type transcripts derived from both yeast tRNA(Arg) and tRNA(Asp) with equal efficiency. It discriminates its cognate natural substrate,...Full Text Available

1996-09-16

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A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism.

Histone-beta-galactosidase protein fusions were used to identify the domain of yeast histone 2B, which targets this protein to the nucleus. Amino acids 28 to 33 in H2B were required for nuclear localization...Full Text Available

1987-11-01

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A New Metal-Binding Site for Yeast Phosphoglycerate Kinase as Determined by the Use of a Metal-ATP Analog

Transcription activation of sulfur metabolism in yeast is dependent on two DNA binding factors, the centromere binding factor 1 (Cbf1) and Met4. While the role of Met4 was clearly established by showing...Full Text Available

1996-05-15

44

Update on the oxidative stress theory of aging: Does oxidative stress play a role in aging or healthy aging?

Suicide substrate β, γ-bidentate Rh(III)ATP (RhATP) was used to map the metal ion-binding site in yeast phosphoglycerate kinase (PGK). Cleavage of the RhATP-inactivated enzyme with...Full Text Available

1997-02-01

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Morphologie des cellules de levure et la reproduction sexuelle - Apercu general et quelques considerations

The oxidative stress theory of aging predicts that manipulations that alter oxidative stress/damage will alter aging. The gold standard for determining whether aging is altered is lifespan,...Full Text Available

2010-03-01

46

British Library Electronic Table of Contents (United Kingdom)

Over the decades, basic research in life sciences has profited greatly from the study of the small unicellular fungal species Saccharomyces cerevisiae. This yeast turned out to be key for the identification and understanding of molecular mechanisms that underlay the basic functions of all eukaryotic cells. These include, but are not limited to, the regulatory mechanisms behind cellular reproduction (cell cycle control), cellular morphogenesis (cell polarity, cytoskeleton and membrane trafficking) and the management of cellular information (chromosome biology, transcription and translation). Rapid access to genomic information of many yeast species, combined with bioinformatics analyses, provide information on the evolutionary history of yeasts and the molecular ancestry of their constituen...

2011-01-01

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Alcohol fermentation in olive oil extraction effluents

Alteration of reactor installation (alteration of No.1 and No.2 reactors) in Sendai Power Station, Kyushu Electric Power Co., Inc. (report)

Eight culture-collection yeast strains of various species and five newly isolated stains were tested for both growth in olive oil extraction effluents and fermentation of the sugars in the same media. The culture-collection yeast strains did not grow in an effluent containing 2.86% sugar (w/v), 8 g/litre phenolic substances, 4.58 g/litre titratable acidity and pH 4.96, whereas the newly isolated strains of Torulopsis sp. MK-1, Saccharomyces norbensis MC-1, S. oleaceus MC-2 and S. oleaginosus grew well and fermented the sugars. In the medium mentioned above, they produced alcohol in amounts of 1.63 to 1.38%, respectively. None of the yeasts grew in an olive oil extraction effluent vacuum-concentrated to over 13-14% of dry matter. The strain of T. sp. MK-1 showed a higher stability.

1989-01-01

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Alteration of reactor installation (alteration of No. 1 and No. 2 reactors) in Sendai Power Station, Kyushu Electric Power Co. , Inc. (report)

Published in summary form only.

1990-01-01

49

Variants within the yeast Ty sequence family encode a class of structurally conserved proteins.

Published in summary form only.

1990-09-01

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The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription

The Ty transposable elements of Saccharomyces cerevisiae form a heterogeneous family within which two broad structural classes (I and II) exist. The two classes differ by two large substitutions and...Full Text Available

1985-06-11

51

The recruitment of acetylated and unacetylated tropomyosin to distinct actin polymers permits the discrete regulation of specific myosins in fission yeast

The mini-chromosome maintenance proteins Mcm2–7 are essential for DNA replication. They are loaded onto replication origins during G1 phase of the cell cycle to form a pre-replication complex...Full Text Available

2005-01-01

52

The fission yeast gene pmt1+ encodes a DNA methyltransferase homologue.

Tropomyosin (Tm) is a conserved dimeric coiled-coil protein, which forms polymers that curl around actin filaments in order to regulate actomyosin function. Acetylation of the Tm N-terminal...Full Text Available

2010-10-01

53

Structure-Function Study of the N-terminal Domain of Exocyst Subunit Sec3*

DNA methylation of cytosine residues is a widespread phenomenon and has been implicated in a number of biological processes in both prokaryotes and eukaryotes. This methylation occurs at the 5-position...Full Text Available

1995-01-25

54

Some highlights of research on aging with invertebrates, 2010

The exocyst is an evolutionarily conserved octameric complex involved in polarized exocytosis from yeast to humans. The Sec3 subunit of the exocyst acts as a spatial landmark for exocytosis through...Full Text Available

2010-04-02

55

SIRT1 contributes to telomere maintenance and augments global homologous recombination

This annual review focuses on invertebrate model organisms, which continue to yield fundamental new insights into mechanisms of aging. This year, the budding yeast has been used to understand how asymmetrical...Full Text Available

2011-02-01

56

PtdIns 3-Kinase Orchestrates Autophagosome Formation in Yeast

Yeast Sir2 deacetylase is a component of the silent information regulator (SIR) complex encompassing Sir2/Sir3/Sir4. Sir2 is recruited to telomeres through Rap1, and this complex spreads into subtelomeric...Full Text Available

2010-12-27

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Overexpression of human virus surface glycoprotein precursors induces cytosolic unfolded protein response in Saccharomyces cerevisiae

Eukaryotic cells can massively transport their own cytoplasmic contents into a lytic compartment, the vacuole/lysosome, for recycling through a conserved system called autophagy. The key process in...Full Text Available

2011-01-01

58

O2-dependent methionine auxotrophy in Cu,Zn superoxide dismutase-deficient mutants of Saccharomyces cerevisiae.

BackgroundThe expression of human virus surface proteins, as well as other mammalian glycoproteins, is much more efficient in cells of higher eukaryotes rather than yeasts. The limitations...Full Text Available

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Nuclear Pore Complex Number and Distribution throughout the Saccharomyces cerevisiae Cell Cycle by Three-Dimensional Reconstruction from Electron Micrographs of Nuclear Envelopes

Mutant strains of the yeast Saccharomyces cerevisiae which lack functional Cu,Zn superoxide dismutase (SOD-1) do not grow aerobically unless supplemented with methionine. The molecular basis of this...Full Text Available

1990-04-01

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Mitochondrial transmission during mating in Saccharomyces cerevisiae is determined by mitochondrial fusion and fission and the intramitochondrial segregation of mitochondrial DNA.

The number of nuclear pore complexes (NPCs) in individual nuclei of the yeast Saccharomyces cerevisiae was determined by computer-aided reconstruction of entire nuclei from electron...Full Text Available

1997-11-01

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Mammalian Sirtuins and Energy Metabolism

To gain insight into the process of mitochondrial transmission in yeast, we directly labeled mitochondrial proteins and mitochondrial DNA (mtDNA) and observed their fate after the fusion of two cells....Full Text Available

1997-07-01

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MAPPING THE INITIATOR BINDING TAF2 SUBUNIT IN THE STRUCTURE OF HYDRATED YEAST TFIID

Sirtuins are highly conserved NAD⁺-dependent protein deacetylases and/or ADP-ribosyltransferases that can extend the lifespan of several lower model organisms including yeast, worms and flies....Full Text Available

63

Legionella fairfieldensis sp. nov. isolated from cooling tower waters in Australia.

SummaryThe general transcription factor TFIID is a large multi-subunit complex required for the transcription of most protein-encoding genes by RNA polymerase II. Taking advantage...Full Text Available

2009-03-11

64

Insights into Cdc13 dependent telomere length regulation

Three Legionella-like organisms were isolated from water from the cooling towers of two Australian institutions. The strains grew on buffered charcoal-yeast extract (BCYE) agar but not on BCYE agar...Full Text Available

1991-03-01

65

Increased Ethanol Productivity in Xylose-Utilizing Saccharomyces cerevisiae via a Randomly Mutagenized Xylose Reductase?

Cdc13 is a single stranded telomere binding protein that specifically localizes to the telomere ends of budding yeasts and is essential for cell viability. It caps the ends of chromosomes thus preventing...Full Text Available

66

Improving yield of industrial biomass propagation by increasing the Trx2p dosage

Baker's yeast (Saccharomyces cerevisiae) has been genetically engineered to ferment the pentose sugar xylose present in lignocellulose biomass. One of the reactions controlling the...Full Text Available

2010-12-01

67

Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels

The beneficial effect of improving yeast redox response by increasing thioredoxin levels has been shown. Decreased lipid and protein oxidation is reflected in an increased biomass yield. In addition,...Full Text Available

2010-09-01

68

Identification and Characterization of CPS1 as a Hyaluronic Acid Synthase Contributing to the Pathogenesis of Cryptococcus neoformans Infection?

Calorie restriction (CR) induces a metabolic shift towards mitochondrial respiration; however, molecular mechanisms underlying CR remain unclear. Recent studies suggest that CR-induced mitochondrial...Full Text Available

69

Fuzzy-decision-making problems of fuel ethanol production using a genetically engineered yeast

Cryptococcus neoformans is a pathogenic yeast that often causes devastating meningoencephalitis in immunocompromised individuals. We have previously identified the C. neoformans...Full Text Available

2007-08-01

70

Fermentability of Corn Syrups with Different Dextrose Equivalents Added to Various Grape Juices¹

A fuzzy-decision-making procedure is applied to find the optimal feed policy of a fed-batch fermentation process for fuel ethanol production using a genetically engineered Saccharomyces yeast 1400 (pLNH33). The policy consisted of feed flow rate, feed concentration, and fermentation time. The recombinant yeast 1400 (pLNH33) can utilize glucose and xylose simultaneously to produce ethanol. However, the parent yeast utilizes glucose only. A partially selective model is used to describe the kinetic behavior of the process. In this study, this partially selective fermentation process is formulated as a general multiple-objective optimal control problem. By using an assigned membership function for each of the objectives, the general multiple-objective optimization problem can be converted into a maximizing decision problem. In order to obtain a global solution, a hybrid method of differential evolution is introduced to solve ...

1998-08-01

71

Expansions, contractions, and fragility of the spinocerebellar ataxia type 10 pentanucleotide repeat in yeast

It was found that neither the enzymes of the grapes nor those of wine yeast Saccharomyces ellipsoideus strain 223 attacked the higher polysaccharides present in corn syrups. The alcohol...Full Text Available

1967-03-01

72

Exit from the Golgi Is Required for the Expansion of the Autophagosomal Phagophore in Yeast Saccharomyces cerevisiae

Spinocerebellar ataxia 10 (SCA10) is an autosomal dominant disease caused by large-scale expansions of the (ATTCT)_n repeat within an intron of the human ATXN10 gene. In contrast...Full Text Available

2011-02-15

73

Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules

The delivery of proteins and organelles to the vacuole by autophagy involves membrane rearrangements that result in the formation of large vesicles called autophagosomes. The mechanism underlying autophagosome...Full Text Available

2010-07-01

74

Cloning and Analysis of a Candida albicans Gene That Affects Cell Surface Hydrophobicity

The programming of cellular networks to achieve new biological functions depends on the development of genetic tools that link the presence of a molecular signal to gene-regulatory activity. Recently,...Full Text Available

2011-07-01

75

Chemical complementation: A reaction-independent genetic assay for enzyme catalysis

The opportunistic pathogenic yeast Candida albicans exhibits growth phase-dependent changes in cell surface hydrophobicity, which has been correlated with adhesion to host tissues....Full Text Available

2001-06-01

76

Biotransformation of Explosives by the Old Yellow Enzyme Family of Flavoproteins

A high-throughput assay for enzyme activity has been developed that is reaction independent. In this assay, a small-molecule yeast three-hybrid system is used to link enzyme catalysis to transcription...Full Text Available

2002-12-24

77

Arabidopsis thaliana Chromosome 4 Replicates in Two Phases That Correlate with Chromatin State

Several independent studies of bacterial degradation of nitrate ester explosives have demonstrated the involvement of flavin-dependent oxidoreductases related to the old yellow enzyme (OYE) of yeast....Full Text Available

2004-06-01

78

Affinity maturation of human botulinum neurotoxin antibodies by light chain shuffling via yeast mating

DNA replication programs have been studied extensively in yeast and animal systems, where they have been shown to correlate with gene expression and certain epigenetic modifications. Despite the conservation...Full Text Available

2010-06-01

79

A Novel Form of Transcriptional Silencing by Sum1-1 Requires Hst1 and the Origin Recognition Complex

Botulism is caused by the botulinum neurotoxins (BoNTs), the most poisonous substance known. Because of the high potency of BoNT, development of diagnostic and therapeutic antibodies for botulism requires...Full Text Available

2010-04-01

80

Yeast as a Model System to Study Tau Biology

In the yeast Saccharomyces cerevisiae, a and α mating-type information is stored in transcriptionally silenced cassettes called HML and HMR....Full Text Available

2001-05-01

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Cell cultures are more sensitive than Saccharamoyces cervisiae tests for assessing the toxicity of aquatic pollutants

Hyperphosphorylated and aggregated human protein tau constitutes a hallmark of a multitude of neurodegenerative diseases called tauopathies, exemplified by Alzheimer's disease. In spite of an enormous amount of research performed on tau biology, several crucial questions concerning the mechanisms of tau toxicity remain unanswered. In this paper we will highlight some of the processes involved in tau biology and pathology, focusing on tau phosphorylation and the interplay with oxidative stress. In addition, we will introduce the development of a human tau-expressing yeast model, and discuss some crucial results obtained in this model, highlighting its potential in the elucidation of cellular processes leading to tau toxicity.

2011-04-06

82

The effect of substrate modification on microbial growth on surfaces

Cultured fish and human cells have been used as bioassay systems for the evaluation of the toxicity of aquatic pollutants. Numerous assays using bacteria and yeast have also been used for such purposes. The authors report the toxicity of aquatic pollutants (Cd, Hg, and Ni), using cell culture systems and the yeast Saccharomyces cervisiae test. Cd, Hg, and Ni were chosen as model compounds of pollutants because the related toxicity is now fairly well established.

1988-07-01

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Cytosolic Calcium Alteration and Cell Injury by Silica in Rat Hepatocytes

The principle aim of the program was to produce a novel, non-leaching antimicrobial surface for commercial development and future use in the liquid food packaging industry. Antimicrobial surfaces which exist presently have been produced to combat the growth of prokaryotic organisms and usually function as slow release systems. A system which could inhibit eukaryotic growth without contaminating the surrounding 'environment' with the inhibitor was considered of great commercial importance. The remit of this study was concerned with creating a surface which could control the growth of eukaryotic organisms found in fruit juice with particular interest in the yeast, Saccharomyces cerevisiae. Putative antimicrobial surfaces were created by the chemical modification of the test substrate polymers; nylon and ethylvinyl alcohol (EVOH). Surfaces were chemically modified by the covalent coupling of antimicrobial agents known to be active against the ...

1998-07-01

84

Ameliorating Effect of Capsaicin on Alterations in Lipid Metabolism during Mice Lung Carcinoma

Full Text Available

1998-12-01

85

Alterations in dopamine and glutamate neurotransmission in tetrahydrobiopterin deficient spr-/- mice: relevance to schizophrenia

Full Text Available

2009-02-01

86

2,4-Dinitrochlorobenzene-induced Atopic Dermatitis Like Immune Alteration in Mice

Full Text Available

2010-09-01

87

Yeast artificial chromosome libraries containing large inserts from mouse and human DNA

Full Text Available

2006-12-01

88

Homology analyses of the protein sequences of fatty acid synthases from chicken liver, rat mammary gland, and yeast

Yeast artificial chromosome (YAC) libraries have been difficult to construct with average insert sizes >400 kilobase pairs when DNA is size-fractionated in low-melting-point agarose. By using yeast chromosomes in mock cloning experiments, the authors found that polyamines should be present whenever agarose containing high molecular weight DNA is melted to protect DNA from degradation. By incorporating polyamines during the cloning procedure, they constructed YAC libraries from mouse and human DNA with average insert sizes of 700 and 620 kilobase pairs, respectively. Several genome equivalents of these YAC libraries were replicated onto the surface of many duplicate agar plates using a 40,000 multipin transfer device. High-density filter replicas were screened by hybridization, and 70 mouse YAC clones from 31 loci and 132 human YAC clones from 49 loci were isolated.

1991-05-15

89

Assessment of the role of oxygen and mitochondria in heat shock induction of radiation and thermal resistance in Saccharomyces cerevisiae

Homology analyses of the protein sequences of chicken liver and rat mammary gland fatty acid synthases were carried out. The amino acid sequences of the chicken and rat enzymes are 67% identical. If conservative substitutions are allowed, 78% of the amino acids are matched. A region of low homologies exists between the functional domains, in particular around amino acid residues 1059-1264 of the chicken enzyme. Homologies between the active sites of chicken and rat and of chicken and yeast enzymes have been analyzed by an alignment method. A high degree of homology exists between the active sites of the chicken and rat enzymes. However, the chicken and yeast enzymes show a lower degree of homology. The DADPH-binding dinucleotide folds of the {beta}-ketoacyl reductase and the enoyl reductase sites were identified by comparison with a known consensus sequence for the DADP- and FAD-binding dinucleotide folds. The active sites of all of the enzymes ...

1989-11-01

90

No Effect of the Altered Peptide Ligand NBI-6024 on ?-Cell Residual Function and Insulin Needs in New-Onset Type 1 Diabetes

In response to a heat shock, the yeast Saccharomyces cerevisiae undergoes a large increase in its resistance to heat and, by the induction of its recombinational DNA repair capacity, a corresponding increase in resistance to radiation. Yeast which lack mitochondrial DNA, mitochondria-controlled protein synthetic apparatus, aerobic respiration, and electron transport (rho/sup 0/ strain) were used to assess the role of O/sub 2/, mitochondria, and oxidative processes controlled by mitochondria in the induction of these resistances. We have found that rho/sup 0/ yeast grown and heat shocked in either the presence or absence of O/sub 2/ are capable of developing both radiation and heat resistance. We conclude that neither the stress signal nor its cellular consequences of induced heat and radiation resistance are directly dependent on O/sub 2/, mitochondrial DNA, or mitochondria-controlled protein synthetic or oxidative ...

1983-10-01

91

Neonatal E. coli infection alters glial, cytokine, and neuronal gene expression in response to acute amphetamine in adolescent rats

OBJECTIVEThis randomized, four-arm, placebo-controlled, dose-ranging phase 2 trial was conducted to determine whether repeated subcutaneous injections of the altered peptide ligand,...Full Text Available

2009-11-01

92

Genetically-engineered mouse models for pancreatic cancer: Advances and current limitations

Neonatal bacterial infection in rats alters the responses to a variety of subsequent challenges later in life. Here we explored the effects of neonatal bacterial infection on a subsequent drug...Full Text Available

2010-04-19

93

Exome sequencing identifies GRIN2A as frequently mutated in melanoma

Recently, there has been significant progress in the development of genetically-engineered mouse (GEM) models. By introducing genetic alterations and/or signaling alterations of human pancreatic cancer...Full Text Available

2011-05-10

94

DNA alterations detected in the progeny of paternally irradiated Japanese medaka fish (Oryzias latipes).

The incidence of melanoma is increasing more than any other cancer, and knowledge of its genetic alterations is limited. To systematically analyze such alterations, we performed whole-exome...Full Text Available

2011-05-01

95

Basement membrane chondroitin sulfate proteoglycan alterations in a rat model of polycystic kidney disease.

A nonmammalian test system for germ-cell mutagenesis has been developed by using the Japanese medaka fish. We describe a system for detecting DNA alterations in F1 progeny descended from the gamma-irradiated...Full Text Available

1995-01-03

96

The Influence of Timber Harvest on the Distribution of Exotic Riparian

Alterations in basement membrane components, notably proteoglycans, in a rat model of polycystic kidney disease have been investigated. Rats were fed phenol II (2-amino-4-hydroxyphenyl-5-phenyl thiazole)...Full Text Available

1994-03-01

97

Removal of uranium from solution using residual brewery yeast: combined biosorption and precipitation

... the Redwood Creek basin in north coastal California, timber harvest and associated streamside landsliding altered the composition ... ...

98

Pectinolytic yeast isolates for cold-active polygalacturonase production

Whilst unwashed preparations of biomass from a local brewery had an apparent maximum biosorption capacity for uranium of 360 mg/g (dry weight biomass) washing reduced this maximum to 150 mg/g. Homogenization of both biomass preparations and recovery of cellular debris had no significant effect on the maximum biosorption capacities although at lower equilibrium concentrations of uranium differences in the biosorption capacities were detected. When unwashed biomass was retained by a semi-permeable membrane 40% of uranium used in the experiments precipitated outside that membrane. Therefore a significant proportion of the uranium removed from solution, and previously attributed to biosorption by the yeast biomass, resulted from precipitation brought about by interaction with low molecular weight components loosely associated with the biomass. (Author).

1997-04-01

99

British Library Electronic Table of Contents (United Kingdom)

Pectin rich cold stored spoiled fruits, vegetables and cold soils were screened and different pectinolytic isolates were obtained by enrichment culturing and ruthenium red plate assay. Among the primary isolates 10-15% were yeast isolates. Six isolates with higher zones of pectin hydrolysis were selected and tested for polygalacturonase (PGU) production at room temperature (25 degrees C) and at 5 degrees C. One isolate identified as Saccharomyces sp. with highest polygalacturonase activity at 5 degrees C was used for enzyme production using raw fruit pectins as substrates. The isolate was identified by preliminary cultural, morphological and sugar fermentation tests. PGU production was high in raw pectin substrates like orange peel (21 U/ml), apple peel (20 U/ml ), mango peel (19 U/ml), ...

2011-01-01

100

Microbiological quality and biophenol content of hot air-dried Thassos cv. table olives upon storage

British Library Electronic Table of Contents (United Kingdom)

Abstract Microbiological quality and biophenol content evolution was studied in minimally processed Thassos cv table olives by hot air dehydration under mild conditions (40C, 24-h, aw-=-0.893) and storage under characteristic packaging conditions (vacuum, 100% N2 and air) at 4 and 20C over a period of 180 days. No salt was used in the production line or packaging. The undesirable microorganisms (Enterobacteriaceae, Staphylococcus aureus, Bacillus, Clostridium) were undetectable until the end of the storage period. Also, modified atmospheres prevented fungal growth at both temperatures apart from the samples stored in air, in which Penicillium and Aspergillus spp. were identified. At 20C, a coexistence of mesophilic bacteria and yeasts occurred. At 4C, yeasts were the predominant microflora...

2011-01-01

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101

Extrinsic allergic alveolitis induced by the yeast Debaryomyces hansenii.

Effects of dietary glucosylceramide on dermatitis in atopic dermatitis model mice

A 65-yr-old female developed cough, fever and dyspnoea following repeated exposure to a home ultrasonic humidifier. High-resolution computed tomography showed ground-glass opacity in both lung fields. Arterial blood gas analysis gave an oxygen tension of 8.38 kPa (63 Torr). Pulmonary function testing revealed restrictive ventilatory impairment with a reduction in the diffusing capacity. The diagnosis of extrinsic allergic alveolitis (EAA) was confirmed by radiographic findings, pathological evidence of alveolitis and reproductive development by a provocation test to the humidifier water. The yeast Debaryomyces Hansenii was the only microorganism cultured from the water of the humidifier. The double diffusion precipitating test and lymphocyte proliferative response was positive for an extract of D. Hansenii, providing evidence to incriminate this fungus. This is the first described case of EAA caused by D. Hansenii. PMID:12449192

2002-11-01

102

British Library Electronic Table of Contents (United Kingdom)

The effects of dietary plant and yeast cerebroside (glucosylceramide), a major sphingolipid in plants and yeast, on atopic dermatitis (AD) like symptoms were investigated in a mouse model. After 7 wk of feeding with a diet containing maize glucosylceramide, plasma IgE levels became significantly lower and in contrast, the levels of interleukin (IL)-12, which induces cellular immunity, became significantly higher in the AD mice than in the controls. However, the sphingolipid constituents of the skin fraction in the maize glucosylceramide fed group did not contain sphingoid bases of plant origin, such as 8-unsaturated sphingoid bases. The results of the present study indicated that dietary plant glucosylceramide prevented AD-like symptoms in AD model mice via regulation of Th1/Th2 balance. P...

2010-01-01

103

Investigation of genomic instability by assay of DNA fingerprint from the offspring of male mice exposed to chronic low-level #gamma#-radiation

Yeast ribosomal protein L1 is required for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits.

By polymerase chain reaction with arbitrary primer (AP-PCR), the possibility of transmission of genome instability to somatic cells of the offspring (F_1 generation) from male parents of mice exposed to chronic low-dose #gamma#-radiation was studied. Male mice 15 days after exposure to 10-50 cGy were mated with unirradiated females. Biopsies were taken from tale tips of two month-old mice progeny for DNA separation. Primer in the AP-PCR was 20-mer oligonucleotide flanking the micro-satellite locus Atplb2 on chromosome 11 of the mouse. Comparative analysis of individual fingerprints of AP-PCR products on DNA-templates from the offspring of irradiated and unirradiated male mice revealed an increased variability of micro-satellite-associated sequences in the genome of the offspring of males exposed to 25 and 50 cGy. DNA-fingerprints of the offspring of male mice exposed to chronic irradiation doses 10 and 25 cGy. 15 days before fertilization (at the post-meiotic stage ...

2000-11-20

104

Wide cross-species aminoacyl-tRNA synthetase replacement in vivo: yeast cytoplasmic alanine enzyme replaced by human polymyositis serum antigen.

Ribosomal protein L1 from Saccharomyces cerevisiae binds 5S rRNA and can be released from intact 60S ribosomal subunits as an L1-5S ribonucleoprotein (RNP) particle. To understand the nature of the...Full Text Available

1993-05-01

105

The k43 gene, required for chorion gene amplification and diploid cell chromosome replication, encodes the Drosophila homolog of yeast origin recognition complex subunit 2

Because of variations in tRNA sequences in evolution, tRNA synthetases either do not acylate their cognate tRNAs from other organisms or execute misacylations which can be deleterious in vivo. We report...Full Text Available

1995-05-23

106

The distribution of active RNA polymerase II along the transcribed region is gene-specific and controlled by elongation factors

Lethal alleles of the Drosophila k43 gene result in small or missing imaginal discs, greatly reduced mitotic index, and fragmented and abnormally condensed chromosomes. A female-sterile...Full Text Available

1997-04-15

107

Specific requirement of the chromatin modifier mSin3B in cell cycle exit and cellular differentiation

In order to study the intragenic profiles of active transcription, we determined the relative levels of active RNA polymerase II present at the 3′- and 5′-ends of 261 yeast genes by...Full Text Available

2010-08-01

108

SWI/SNF and Asf1 Independently Promote Derepression of the DNA Damage Response Genes under Conditions of Replication Stress

The Sin3-histone deacetylase (HDAC) corepressor complex is conserved from yeast to humans. Mammals possess two highly related Sin3 proteins, mSin3A and mSin3B, which serve as scaffolds tethering HDAC...Full Text Available

2008-03-18

109

Primary organization of nucleosomal core particles is invariable in repressed and active nuclei from animal, plant and yeast cells.

The histone chaperone Asf1 and the chromatin remodeler SWI/SNF have been separately implicated in derepression of the DNA damage response (DDR) genes in yeast cells treated with genotoxins that cause...Full Text Available

110

A refined map for the linear arrangement of histones along DNA in nucleosomal core particles has been determined by DNA-protein crosslinking. On one strand of 145-bp core DNA, histones are aligned in...Full Text Available

1985-05-24

111

Molecular methods

Metabolomic analysis of the plant pathogenic fungi Fusarium graminearum and Fusarium culmorum

Chapter 5, describes some of the most important molecular methods used in the study of chromosome structure and function. The methods discussed include fragmentation of DNA, cloning, flow cytometry and chromosome sorting, is situ hybridization, polymerase chain reaction (PCR), and yeast artificial chromosomes (YACs). 18 refs., 3 figs., 1 tab.

1993-12-31

112

Environmental Research Database

DescriptionThis project is part of the BBSRCs special initiative on plant and microbial metabolomics. The project will primarily focus on the trichothecene mycotoxin producing Ascomycete fungus Fusarium graminearum (Fg) which causes ear blight disease of small grain cereals. The project aims to explore the metabolome of various wild-type and single gene deletion Fg strains and to compare some of these with the identical gene mutation in the budding yeast, S. cerevisiae (Sc) and the saprophytic filamentous [continued...

2008-01-31

113

Hybridization with synthetic oligonucleotides

Genome-wide analysis of N¹-methyl-adenosine modification in human tRNAs

Procedures are described for the use of synthetic oligonucleotides for Southern blot experiments and gene bank screening, and the effect of various mismatches on the efficiency of hybridization is demonstrated. The following topics are discussed: sensitivity vs. specificity, hybridization of a 12-mer to the lambda endolysin gene; hybridization of oligonucleotide probes to the E. coli lac operator; hybridization of synthetic probes to the CYC1 gene of yeast; and cloning eucaryotic genes. (HLW)

1978-01-01

114

Functional and Topological Analysis of Yeast Acyl-CoA:Diacylglycerol Acyltransferase 2, an Endoplasmic Reticulum Enzyme Essential for Triacylglycerol Biosynthesis*

The N¹-methyl-Adenosine (m¹A58) modification at the conserved nucleotide 58 in the TΨC loop is present in most eukaryotic tRNAs. In yeast, m¹A58 modification...Full Text Available

2010-07-01

115

Enhanced Degradation of an Endocrine-Disrupting Chemical, Butyl Benzyl Phthalate, by Fusarium oxysporum f. sp. pisi Cutinase

Acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20) is a membrane protein present mainly in the endoplasmic reticulum. It catalyzes the final and committed step in the biosynthesis of triacylglycerol,...Full Text Available

2011-04-15

116

Biological solubilization of coal in aqueous and nonaqueous media

Compared to yeast esterase, fungal cutinase degraded butyl benzyl phthalate (BBP) far more efficiently; i.e., almost 60% of the BBP disappeared within 7.5 h. Also, the final chemical composition significantly...Full Text Available

2002-09-01

117

Assembly of 60S ribosomal subunits is perturbed in temperature-sensitive yeast mutants defective in ribosomal protein L16.

Current investigations into the biological solubilization of coal with microorganisms focus on the production of solubilizing activity in fungi. Test organisms for this work include a species of the yeast Candida previously isolated from a lignite outcrop, and P. chrysosporium, a filamentous higher fungus which has played a major role in lignin biodegradation research. The studies described are primarily exploratory in nature, and are fundamental to the design of more sophisticated inquiries into the physiology of fungal coal solubilization.

1987-01-01

118

Tobacco-induced alterations to Porphyromonas gingivalis-host interactions

Temperature-sensitive mutants defective in 60S ribosomal subunit protein L16 of Saccharomyces cerevisiae were isolated through hydroxylamine mutagenesis of the RPL16B gene and plasmid shuffling. Two...Full Text Available

1991-11-01

119

The effect of aqueous composition on diffusion coefficient in bentonite

SUMMARYSmokers are more susceptible than non-smokers to persistent infection by Porphyromonas gingivalis, a causative agent of periodontitis. Patients who smoke...Full Text Available

2009-05-01

120

The Importance of Mitochondrial DNA in Aging and Cancer

The diffusion coefficients (De) in bentonite were measured to understand and quantify the influence of groundwater chemistry such as ionic strength and to quantify the alteration of smectite mineralogical transformations. (author)

2009-12-01

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121

Telomeres and telomerase in cancer

Mitochondrial dysfunction has been implicated in premature aging, age-related diseases, and tumor initiation and progression. Alterations of the mitochondrial genome accumulate both in aging tissue...Full Text Available

122

Structural alterations in the seminiferous tubules of rats treated with immunosuppressor tacrolimus

Myriad genetic and epigenetic alterations are required to drive normal cells toward malignant transformation. These somatic events commandeer many signaling pathways that cooperate to endow aspiring...Full Text Available

2010-01-01

123

BackgroundTacrolimus (FK-506) is an immunosuppressant that binds to a specific immunophilin, resulting in the suppression of the cellular immune response during transplant rejection....Full Text Available

124

Stickiness to Glass

Site-Specific Methylation of the Promoter Alters Deoxyribonucleic Acid-Protein Interactions and Prevents ...

Conditions were found in which Chlamydomonas reinhardi exhibits a circadian alteration of its cell surface, measured as ability to stick to glass. Under these same conditions the cells...Full Text Available

1979-06-01

125

... and R. L. Momparler. DNA methylation of retinoic acid receptor beta in breast cancer and possible therapeutic role of ... ...

126

Reversing B cell aging

Regulation of Glutamate Dehydrogenases in nit-2 and am Mutants of Neurospora crassa

Age-related alterations in the cellular composition of the B lineage are a major cause of the poor antibody response to vaccination and to infectious agents among the elderly population. The mechanisms...Full Text Available

127

Principles of antibody therapy.

The regulation of the glutamate dehydrogenases was investigated in wild-type Neurospora crassa and two classes of mutants altered in the assimilation of inorganic nitrogen, as either...Full Text Available

1979-03-01

128

Prenatal immunotoxicant exposure and postnatal autoimmune disease.

The success of monoclonal antibodies in clinical practice is dependent on good design. Finding a suitable target is the most important part as other properties of the antibody can be altered by genetic...Full Text Available

1992-12-05

129

Immune Alteration Studies in Irradiated Dogs.

Reports in humans and rodents indicate that immune development may be altered following perinatal exposure to immunotoxic compounds, including chemotherapeutics, corticosteroids, polycyclic hydrocarbons,...Full Text Available

1999-10-01

130

HDL in humans with cardiovascular disease exhibits a proteomic signature

... objective of this research work were to 1 ... function of experimentally manipulated dogs to define ... Escherichia coli; Sepsis; Military medicine; Medical ...

1986-10-31

131

Fractal dynamics in physiology: Alterations with disease and aging

BackgroundAlterations in protein composition and oxidative damage of high-density lipoprotein (HDL) have been proposed to impair the cardioprotective properties of...Full Text Available

2010-07-04

132

Environmental estrogens alter early development in Xenopus laevis.

According to classical concepts of physiologic control, healthy systems are self-regulated to reduce variability and maintain physiologic constancy. Contrary to the predictions of homeostasis, however,...Full Text Available

2002-02-19

133

Copper deficiency alters the neurochemical profile of developing rat brain

A growing number of environmental toxicants found in pesticides, herbicides, and industrial solvents are believed to have deleterious effects on development by disrupting hormone-sensitive processes....Full Text Available

2003-04-01

134

Cardiac Enzymes, Renal Failure and Renal Transplantation

Copper deficiency is associated with impaired brain development and mitochondrial dysfunction. Perinatal copper deficiency was produced in Holtzman rats. In vivo proton NMR...Full Text Available

2009-06-01

135

Age and the Thyrotropin Response to Hypothyroxinemia

Diagnostic accuracy of the currently available serum markers of cardiac injury, such as myoglobin, creatine kinase and its myocardial isoform, are altered in patients with renal failure. It is shown...Full Text Available

2006-03-01

136

Abnormal chromosome repair and risk of developing cancer.

Context: Some studies suggest altered pituitary functioning and TSH production with aging.Objective: Our objective was to test the hypothesis that less TSH production...Full Text Available

2010-08-01

137

AMPA-receptor trafficking and injury-induced cell death

Several scientists have proposed that DNA repair deficiencies and the induction of a mutator phenotype are responsible for the generation of multiple mutagenic alterations in cancer cells. I propose...Full Text Available

1993-10-01

138

Mutations in cyr1 and pat1 reveal pheromone-induced G1 arrest in the fission yeast Schizosaccharomyces pombe

AMPA receptors (AMPARs) are critical for synaptic plasticity, and are subject to alterations based on subunit composition and receptor trafficking to and from the plasma membrane. One of the...Full Text Available

2010-07-01

139

DEFF Research Database (Denmark)

Investigations into sexual differentiation and pheromone response in the fission yeast Schizosaccharomyces pombe are complicated by the need to first starve the cells of nitrogen. Most mating-related experiments are therefore performed on non-dividing cells. Here we overcome this problem by using two mutants that bypass the nutritional requirements and respond to the M-factor mating pheromone in rich medium. The first mutant lacks the cyr1 gene which encodes adenylate cyclase and these cells contain no measurable amounts of cAMP. When M-factor is added to a growing h+ cyr1- strain it causes a transient G1 arrest of cell division, transcription of mat1-Pm, and elongation of the cells to form shmoos. The second mutant contains the temperature-sensitive pat1-114 allele. At 30 degrees C this mutant was previously shown not only to bypass the nutritional signal but also to stop growing in a state derepressed for pheromone-controlled functions. We now report that an h+ ...

1994-01-01

140

Mitochondrial genetic damage induced in yeast by a photoactivated furocoumarin in combination with ethidium bromide or ultraviolet light

Management of industrial solid wastes in Alexandria, Egypt

Ethidium bromide (EB) and ultraviolet light (UV) in combination are known to produce a synergistic induction of 'petite' mutants in yeast. Two other agents were combined with EB, 3-Carbethoxypsoralene (3 CPs) activated by 365 nm light or #gamma# rays. EB in combination with 3 CPs also resulted in an enhanced production of 'petite' mutants. After the photoaddition of 3 CPs in exponential phase cells, recovery of the 'petite' mutation during dark liquid holding was inhibited by the presence of EB producing an enhanced number of 'petite' mutants. The behavior of mitochondrial antibiotic resistance markers after individual and combined treatments with EB and 3 CPs indicates a random loss of markers after EB and a preferential loss of a certain region for the 3 CPs photoaddition. The combination of the two agents leads to an additivity of total drug marker losses rather than a synergistic loss. The combination of EB with #gamma# rays produced no enhancement in 'petite' ...

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141

Mineral biotechnology. Microbial aspects of mineral beneficiation, metal extraction, and environmental control

This paper presents a summary of the first phase of the EPA project, which encompasses surveys of residues from industrial sources in Alexandria. Studies to date indicate that wastes from various industries can be recovered economically. Wastes such as tin cans, glass, wastepaper, and food residues from processing of fruits, starch, and beer are examples of reusable industrial wastes in Egypt. The results of experimental studies for reuse of residues from oil refining, starch and yeast processing, and steel pickling are presented. Spent clay from edible oil refining is currently discarded, causing both handling and disposal problems. This clay contains as much as 40% oil; 90% can be recovered by extraction. The recovered oil can be successfully used in soap production, and the spent clay can be reused in oil bleaching. Other examples include starch and yeast wastes, which can be used for animal feed, and spent pickling liquor, which can be used ...

1983-03-01

142

Mechanism of biodegradation of paraquat by Lipomyces starkeyi

Papers in this book illustrate the utility of mineral biotechnology with respect to biobeneficiation, bioleaching, bioremediation and biomineralization. Papers of particular interest to the coal industry include: depression of pyrite flotation by yeast and bacteris (S.K. Kawatra and T.C. Eisele); desulfurization of coal by microbial flotation in a semicontinuous system (T. Nagaoka and others); biochemical removal of HAP precursors from coal - INEEL slurry column testing (K.S. Noah and G.J. Olson); microorganisms, biotechnology and acid rock drainage - emphasis on passive-biological control and treatment methods (N. Kuyucak); and utility of bioreagents in mineral processing (P. Somasundaran and others).

2001-07-01

143

Mechanism of biodegradation of paraquat by Lipomyces starkeyi

The biodegradation of ring-/sup 14/C- and methyl-/sup 14/C-labeled paraquat by the soil yeast Lipomyces starkeyi was studied in vitro. It was found that the degradation of paraquat (acting as a sole source of culture nitrogen) resulted in the accumulation in the extracellular medium of radiolabeled acetic acid. The culture also evolved radiolabeled CO/sub 2/. The results suggest that the degradation of paraquat by L. starkeyi is associated with the integrity of the cell wall and that disruption or removal of the wall results in a complete loss of degradative capability. A mechanism for the degradation of paraquat by this organism is postulated.

1985-05-01

144

Latvian scientists research into chemical uses of timber

The biodegradation of ring-"1"4C- and methyl-"1"4C-labeled paraquat by the soil yeast Lipomyces starkeyi was studied in vitro. It was found that the degradation of paraquat (acting as a sole source of culture nitrogen) resulted in the accumulation in the extracellular medium of radiolabeled acetic acid. The culture also evolved radiolabeled CO_2. The results suggest that the degradation of paraquat by L. starkeyi is associated with the integrity of the cell wall and that disruption or removal of the wall results in a complete loss of degradative capability. A mechanism for the degradation of paraquat by this organism is postulated.

145

Antifungal activity of Glycyrrhiza glabra extracts and its active constituent glabridin

Scientists of the Institute of Wood Chemistry of the Latvian Academy of Sciences have developed two highly efficient processes for producing furfural, a feedstock for varnishes, synthetic resins and plastics. It is made of production wastes, including branches and small-dimension timber. By one process, the raw material is chipped, treated first with diluted sulphuric acid and then with steam heated to 250 degrees C. The other uses concentrated sulphuric acid as a catalyst. Besides furfural, this process also yields sugar solutions used in alcohol and nutrient yeast production.

1982-03-20

146

British Library Electronic Table of Contents (United Kingdom)

Glabridin, an active constituent of Glycyrrhiza glabra roots, was found to be active against both yeast and filamentous fungi. Glabridin also showed resistance modifying activity against drug resistant mutants of Candida albicans at a minimum inhibitory concentration of 31.25-250 g/mL. Although the compound was reported earlier to be active against Candida albicans, but this is the first report of its activity against drug resistant mutants. Copyright Copyright 2009 John Wiley & Sons, Ltd.

2009-01-01

147

Antifungal activity of the extracts and neolignans from Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck

Transgenerational Effects of Di (2-Ethylhexyl) Phthalate in the Male CRL:CD(SD) Rat: Added Value of Assessing Multiple Offspring per Litter

Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck (Piperaceae) is a medicinal plant traditionally used in Brazil to treat infectious diseases. The extracts obtained from the leaves of P. regnellii were investigated for their antifungal activities against the yeasts Candida albicans, C. krusei, C. parapsilosis, and C. tropicalis. The EtOAc extract presented gnificant activity against Candida albicans with MIC at 125 {mu}g mL{sup -1}, and a moderate activity against both C. krusei and C. parapsilosis with MIC at 500 {mu}g mL{sup -1}. Candida tropicalis was not inhibited by this extract at concentrations as high as 1000 {mu}g mL{sup -1}. Based on these findings, the EtOAc extract was fractionated by silica gel column chromatography into nine fractions. The hexane and CHCl{sub 3} fractions showed varied levels of antifungal activity against all test yeasts. Further column chromatography separation of the hexane fraction afforded the pure ...

2005-11-15

148

A single early life seizure impairs short-term memory but does not alter spatial learning, recognition memory, or anxiety

In the rat, some phthalates alter sexual differentiation at relatively low dosage levels by altering fetal Leydig cell development and hormone synthesis, thereby inducing abnormalities of the testis,...Full Text Available

2009-08-01

149

A System-Based Comparison of Gene Expression Reveals Alterations in Oxidative Stress, Disruption of Ubiquitin-Proteasome System and Altered Cell Cycle Regulation after Exposure to Cadmium and Methylmercury in Mouse Embryonic Fibroblast

The impact of a single seizure on cognition remains controversial. We hypothesized that a single early life seizure (sELS) on rat post-natal day (P) 7 would alter only hippocampal-dependent...Full Text Available

2008-11-01

150

Alteration in reactor installation (addition of Unit 2) in the Sendai Nuclear Power Station of Kyushu Electric Power Co., Inc

Environmental and occupational exposures to heavy metals such as methylmercury (MeHg) and cadmium (Cd) pose significant health risks to humans, including neurotoxicity. The underlying mechanisms of...Full Text Available

2010-04-01

151

Alteration in reactor installation (addition of Unit 2) in the Sendai Nuclear Power Station of Kyushu Electric Power Co. , Inc

The deliveration by the Nuclear Safety Commission was commenced on the alteration in reactor installation, as it had been inquired by the Ministry of International Trade and Industry. The alteration is the additional installation of the reactor No. 2 in the Sendai Nuclear Power Station, Kyushu Electric Power Co., Inc. It is a PWR power plant with thermal output of about 2,660 MW (electric output of 890 MW), to be installed, adjoining to the reactor No. 1 of the same type and capacity under construction. In the examination by MITI, it was confirmed that the technological capabilities for its construction and operation and the radiation protection measures in power generation are both sufficient. The contents of the examination include the siting conditions, the location and construction of reactor facilities, etc. (J.P.N.).

1980-01-01

152

ras gene alterations in invasive and non-invasive rat bladder carcinomas induced by N-methyl-N-nitrosourea.

The deliberation by the Nuclear Safety Commission was initiated on the alteration in reactor installation, as was required by the Ministry of International Trade and Industry. The alteration is the additional installation of the reactor No. 2 in the Sendai Nuclear Power Station, Kyushu Electric Power Co., Inc. It is a PWR power plant with thermal output of about 2,660 MW (electric output of 890 MW), to be installed, adjoining to the reactor No. 1 of the same type and capacity under construction. In the examination by MITI, it was confirmed that the technological capabilities for its construction and operation and the radiation protection measures in power generation are both sufficient. The contents of the examination include the siting conditions, the location and construction of reactor facilities, etc.

1980-10-01

153

Upregulation of prolylcarboxypeptidase (PRCP) in lipopolysaccharide (LPS) treated endothelium promotes inflammation

We have established a reliable method to induce invasive and non-invasive carcinomas in the heterotopically transplanted urinary bladder of rats by repeated injection of N-methyl-N-nitrosourea (MNU),...Full Text Available

1991-07-01

154

Tumour-stromal interactions: Phenotypic and genetic alterations in mammary stroma - implications for tumour progression

BackgroundProlylcarboxypeptidase (Prcp) gene, along with altered PRCP and kallikrein levels, have been implicated in inflammation pathogenesis. PRCP regulates angiotensin...Full Text Available

155

Transplantation of allogeneic T cells alters iron homeostasis in NOD/SCID mice

In addition to the well documented role of cytokines in mediating tissue-level interactions, it is now clear that matrix macromolecules fulfil a complementary regulatory function. Data highlighted in...Full Text Available

2001-01-01

156

The protozoan parasite Theileria annulata alters the differentiation state of the infected macrophage and suppresses musculoaponeurotic fibrosarcoma oncogene (MAF) transcription factors

Iron overload is common in patients undergoing allogeneic hematopoietic cell transplantation (HCT), but the mechanisms leading to overload are unknown. Here, we determined iron levels and the expression...Full Text Available

2009-02-19

157

The protein encoded by the rolB plant oncogene hydrolyses indole glucosides.

AbstractThe tick-borne protozoan parasite Theileria annulata causes a debilitating disease of cattle called Tropical Theileriosis. The parasite predominantly invades...Full Text Available

2009-08-01

158

The orphan nuclear receptor small heterodimer partner mediates male infertility induced by diethylstilbestrol in mice

The rolB gene of Agrobacterium rhizogenes, whose expression stimulates the formation of roots by transformed plant tissues and other growth alterations in transgenic plants, codes for a beta-glucosidase...Full Text Available

1991-11-01

159

The long lifespan of two bat species is correlated with resistance to protein oxidation and enhanced protein homeostasis

Studies in rodents have shown that male sexual function can be disrupted by fetal or neonatal administration of compounds that alter endocrine homeostasis, such as the synthetic nonsteroidal estrogen...Full Text Available

2009-12-01

160

The Role of Thyroid Hormone in Testicular Development and Function

Altered structure, and hence function, of cellular macromolecules caused by oxidation can contribute to loss of physiological function with age. Here, we tested whether the lifespan of bats, which generally...Full Text Available

2009-07-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

161

The Effects of Time Varying Curvature on Species Transport in Coronary Arteries

Thyroid hormone is a critical regulator of growth, development and metabolism in virtually all tissues, and altered thyroid status affects many organs and systems. Although for many years testis...Full Text Available

2008-12-01

162

The Effect of Age on the Gingival Crevicular Fluid Composition During Experimental Gingivitis. A Pilot Study

Alterations in mass transport patterns of low-density lipoproteins (LDL) and oxygen are known to cause atherosclerosis in larger arteries. We hypothesise that the species transport processes in coronary...Full Text Available

2006-12-01

163

The Cell Non-Autonomous Nature of Electron Transport Chain-Mediated Longevity

Background:Cytokines have been proposed as potentially useful diagnostic or prognostic markers of periodontal inflammation related alterations during the experimental gingivitis...Full Text Available

164

The Arabidopsis ref2 Mutant Is Defective in the Gene Encoding CYP83A1 and Shows Both Phenylpropanoid and Glucosinolate Phenotypes

SummaryThe life span of C. elegans can be increased via reduced function of the mitochondria; however, the extent to which mitochondrial alteration in a single,...Full Text Available

2011-01-07

165

Surprising Alteration of Antibacterial Activity of 5?-Modified Neomycin against Resistant Bacteria

The Arabidopsis ref2 mutant was identified in a screen for plants having altered fluorescence under UV light. Characterization of the ref2 mutants showed that they...Full Text Available

2003-01-01

166

Structural analysis of a hepatitis B virus genome integrated into chromosome 17p of a human hepatocellular carcinoma.

A facile synthetic protocol for the production of neomycin B derivatives with various modifications at the 5″ position has been developed. Structural activity relationship (SAR) against...Full Text Available

2008-12-11

167

SirT3 suppresses hypoxia inducible factor 1? and tumor growth by inhibiting mitochondrial ROS production

Hepatitis B virus (HBV) is clearly a factor in the development of hepatocellular carcinoma, but its mechanism of action remains obscure. One possibility is that the HBV integration event alters the...Full Text Available

1988-11-01

168

Serotonin Transporter Binding and Genotype in the Nonhuman Primate Brain using [C-11]DASB PET

It has become increasing clear that alterations in cellular metabolism have a key role in the generation and maintenance of cancer. Some of the metabolic changes can be attributed to the activation...Full Text Available

2011-06-30

169

Role of interferon-gamma in interleukin 12-induced pathology in mice.

The length polymorphism of the serotonin (5-HT) transporter gene promoter region has been implicated in altered 5-HT function and, in turn, neuropsychiatric illnesses, such as anxiety and depression....Full Text Available

2009-10-01

170

Reciprocal Silencing, Transcriptional Bias and Functional Divergence of Homeologs in Polyploid Cotton (Gossypium)

Interleukin 12 (IL-12) activates natural killer (NK) and T cells with the secondary synthesis and release of interferon-gamma (IFN-gamma) and other cytokines. IL-12-induced organ alterations are reported...Full Text Available

1995-12-01

171

Quantitative Analysis of Endocytosis and Turnover of Epidermal Growth Factor (EGF) and EGF Receptor

Polyploidy is an important force in the evolution of flowering plants. Genomic merger and doubling induce an extensive array of genomic effects, including immediate and long-term alterations in the...Full Text Available

2009-06-01

172

Proliferative Hypothalamic Neurospheres Express NPY, AGRP, POMC, CART and Orexin-A and Differentiate to Functional Neurons

Binding of epidermal growth factor (EGF) to the EGF receptor (EGFR) initiates signal transduction, ultimately leading to altered gene expression. Ligand-activated EGFR is also rapidly internalized...Full Text Available

2010-03-01

173

Progressive ratio performance following challenge with antipsychotics, amphetamine, or NMDA antagonists in adult rats treated perinatally with phencyclidine

Some pathological conditions with feeding pattern alterations, including obesity and Huntington disease (HD) are associated with hypothalamic dysfunction and neuronal cell death. Additionally, the hypothalamus...Full Text Available

174

Presence of preactivated T cells in hemodialyzed patients: their possible role in altered immunity.

RationalePrevious research has shown that rats exposed perinatally to phencyclidine (PCP) exhibited neuroanatomical abnormalities and altered cognition. In addition...Full Text Available

2004-12-01

175

Phytoestrogens: epidemiology and a possible role in cancer protection.

Interleukin 2 (IL-2) and B-cell growth factors I and II (BCGF I and BCGF II) are lymphokines produced by T cells that play a major role in T- and B-cell cooperation. Peripheral blood lymphocytes from...Full Text Available

1986-10-01

176

Osmotic Shock Inhibits Auxin-stimulated Acidification and Growth ¹

Because many diseases of the Western Hemisphere are hormone-dependent cancers, we have postulated that the Western diet, compared to a vegetarian or semivegetarian diet, may alter hormone production,...Full Text Available

1995-10-01

177

Non-Invasive Stem Cell Therapy in a Rat Model for Retinal Degeneration and Vascular Pathology

Cells of oat coleoptiles (Avena sativa L. cv. “Garry”) have been osmotically shocked in order to observe the effect of alterations of the plasma membrane on some auxin...Full Text Available

1977-03-01

178

New retinal imaging for the visualization and analysis of vitreoretinal interface (VRI) by short-wavelength scanning laser ophthalmoscope (swSLO)

BackgroundRetinitis pigmentosa (RP) is characterized by progressive night blindness, visual field loss, altered vascular permeability and loss of central vision. Currently there...Full Text Available

179

Neuronatin: A New Inflammation Gene Expressed on the Aortic Endothelium of Diabetic Mice

Background:The purpose of the study was to evaluate vitreoretinal interface (VRI) alteration with a short-wavelength scanning laser ophthalmoscope (swSLO), the Nidek F-10, and compare...Full Text Available

2011-01-01

180

Neonatal Alcohol Exposure Differentially Alters Clock Gene Oscillations Within the Suprachiasmatic Nucleus, Cerebellum, and Liver of Adult Rats

OBJECTIVE—Identification of arterial genes and pathways altered in obesity and diabetes.RESEARCH DESIGN AND METHODS—Aortic gene expression profiles of...Full Text Available

2008-10-01

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181

Move or Die: the Fate of the Tax Oncoprotein of HTLV-1

BackgroundIn rats, alcohol exposure during the period of rapid brain growth produces long-term changes in the free-running period, photoentrainment and phase-shifting...Full Text Available

2008-03-01

182

Mouse model predicts effects of smoking and varenicline on event-related potentials in humans

The HTLV-1 Tax protein both activates viral replication and is involved in HTLV-1-mediated transformation of T lymphocytes. The transforming properties of Tax include altering the expression of select...Full Text Available

183

Modulation of leg muscle function in response to altered demand for body support and forward propulsion during walking

Background:Nicotine alters auditory event-related potentials (ERPs) in rodents and humans and is an effective treatment for smoking cessation. Less is known about the effects of...Full Text Available

2010-06-01

184

Modular control of human walking: Adaptations to altered mechanical demands

A number of studies have examined the functional roles of individual muscles during normal walking, but few studies have examined which are the primary muscles that respond to changes in external...Full Text Available

2009-05-11

185

Moderate Caloric Restriction during Gestation in Rats Alters Adipose Tissue Sympathetic Innervation and Later Adiposity in Offspring

Studies have shown that the nervous system may adopt a control scheme in which synergistic muscle groups are controlled by common excitation patters, or modules, to simplify the coordination...Full Text Available

2010-02-10

186

Methods to Quantify Pharmacologically Induced Alterations in Motor Function in Human Incomplete SCI

Maternal prenatal undernutrition predisposes offspring to higher adiposity in adulthood. Mechanisms involved in these programming effects, apart from those described in central nervous system development,...Full Text Available

187

Membrane alteration is necessary but not sufficient for effective glutamate secretion in Corynebacterium glutamicum.

Spinal cord injury (SCI) is a debilitating disorder, which produces profound deficits in volitional motor control. Following medical stabilization, recovery from SCI typically involves long term rehabilitation....Full Text Available

188

Loss of red cell chemokine scavenging promotes transfusion-related lung inflammation

We showed recently that secretion of glutamate in biotin-limited cells of Corynebacterium glutamicum is mediated by carrier systems in the plasma membrane (C. Hoischen and R. Krämer, Arch. Microbiol....Full Text Available

1990-06-01

189

Large-scale cross-species oncogenomics identifies candidate oncogenes and tumor suppressor genes

Red cell transfusions are associated with the development of acute lung injury in the critically ill. Recent evidence suggests that storage induced alterations of the red blood cell (RBC) collectively...Full Text Available

2009-01-29

190

Intrinsic plasticity complements LTP in parallel fiber input gain control in cerebellar Purkinje cells

While genomic alterations identified in human tumors using techniques such as comparative genomic hybridisation (CGH) may be recurrent, they frequently encompass large regions, in some cases...Full Text Available

2010-02-01

191

Intrinsic expression of host genes and intronic miRNAs in prostate carcinoma cells

Synaptic gain control and information storage in neural networks are mediated by alterations in synaptic transmission, such as in long-term potentiation (LTP). Here, we show using both in...Full Text Available

2010-10-13

192

Interactive Toxicity of Inorganic Mercury and Trichloroethylene in Rat and Human Proximal Tubules

BackgroundRecent data show aberrant and altered expression of regulatory noncoding micro (mi) RNAs in prostate cancer (PCa). A large number of miRNAs are encoded in organized intronic...Full Text Available

193

Insect growth regulators and insect control: a critical appraisal.

Simultaneous or prior exposure to one chemical may alter the concurrent or subsequent response to another chemical, often in unexpected ways. This is particularly true when the two chemicals...Full Text Available

2007-06-15

194

Inhibition of lactate dehydrogenase A induces oxidative stress and inhibits tumor progression

Insect growth regulators (IGRs) of the juvenile hormone type alter physiological processes essential to insect development and appear to act specifically on insects. Three natural juvenile hormones...Full Text Available

1976-04-01

195

In vivo cartilage contact strains in patients with lateral ankle instability

As the result of genetic alterations and tumor hypoxia, many cancer cells avidly take up glucose and generate lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc...Full Text Available

2010-02-02

196

Impact of repeated four-monthly anthelmintic treatment on Plasmodium infection in preschool children: a double-blind placebo-controlled randomized trial

Damage to the anterior talofibular ligament (ATFL) and cacaneofibular ligament (CFL) during ankle sprain may be linked to the development of osteoarthritis. Altered tibiotalar kinematics have...Full Text Available

2010-09-17

197

Impact of Common Medications on Serum Total Prostate-Specific Antigen Levels: Analysis of the National Health and Nutrition Examination Survey

BackgroundHelminth infections can alter susceptibility to malaria. Studies need to determine whether or not deworming programs can impact on Plasmodium infections...Full Text Available

198

Glucocorticoids exacerbate hypoxia induced expression of the pro-apoptotic gene Bnip3 in the developing cortex

PurposePrevious studies suggest that some common medications alter prostate-specific antigen (PSA) levels. It remains unclear whether these reported medication effects are due to...Full Text Available

2010-09-01

199

Fourier-Domain Optical Coherence Tomography and Adaptive Optics Reveal Nerve Fiber Layer Loss and Photoreceptor Changes in a Patient With Optic Nerve Drusen

Neonatal administration of the synthetic glucocorticoid, dexamethasone (DEX) retards brain growth, alters adult behaviors and induces cell death in the rat brain, thereby implicating glucocorticoids...Full Text Available

2007-01-19

200

Fetal, perinatal, and infant death with congenital renal anomaly

BackgroundNew technology allows more precise definition of structural alterations of all retinal layers although it has not been used previously in cases of optic...Full Text Available

2008-06-01

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201

Expression of CD1d in human scalp skin and hair follicles: hair cycle related alterations

Aims: To ascertain why 19.6% of pregnancies in which a fetal renal anomaly has been detected fail to produce a surviving child, and whether antenatal diagnostic accuracy has altered since...Full Text Available

2002-08-01

202

Exercise alters SIRT1, SIRT6, NAD and NAMPT levels in skeletal muscle of aged rats

Background: CD1d belongs to a family of antigen presenting molecules that are structurally and distantly related to the classic major histocompatibility complex class I (MHC I)...Full Text Available

2005-12-01

203

Electromigration study of focused ion beam modified metal lines

Silent information regulators are potent NAD⁺-dependent protein deacetylases, which have been shown to regulate gene silencing, muscle differentiation and DNA damage repair. Here,...Full Text Available

2010-01-01

204

Effects of exposure to low concentrations of chlorinated hydrocarbons on the kidney and liver of industrial workers.

Focus ion beam (FIB) technology is a commonly used tool for integrated circuit device modification, failure analysis, and a variety of other applications. However, limited reliability data of FIB altered circuit is available. This study describes the electromigration mechanism of FIB-altered Al(Cu-Si)/Ti-layered metal lines. The electromigration failures encountered resulted in Al voids at the anode end of FIB-deposited W and Al{sub 2}Cu precipitates at the cathode end. A higher frequency of Al extrusions was also observed on samples stressed at higher temperatures. These observations indicated that FIB-W was an effective blocking boundary for Al and Cu, and confirmed that Cu does electromigrate before Al. The electromigration mechanism of the FIB-altered line closely resembles the published results from two-level Al/W-line/stud interconnect studies. An activation energy of 0.66 eV, with a current exponent of 1.9 and a ...

1995-12-31

205

Effects of cyclooxygenase inhibitors on the alterations in lung mechanics caused by endotoxemia in the unanesthetized sheep.

An assessment has been made of biochemical alterations in renal and hepatic functions of 73 male operators employed for an average of 8.2 years (range 0.5-23 years) in a chemical plant producing chlorinated...Full Text Available

1993-04-01

206

Effects of Kombucha on oxidative stress induced nephrotoxicity in rats

The effects of Escherichia coli endotoxin on lung mechanics, hemodynamics, gas exchange, and lung fluid and solute exchange were studied in 12 chronically instrumented unanesthetized sheep. A possible...Full Text Available

1983-07-01

207

Effect of neonatal exposure to estrogenic compounds on development of the excurrent ducts of the rat testis through puberty to adulthood.

BackgroundTrichloroethylene (TCE) may induce oxidative stress which generates free radicals and alters antioxidants or oxygen-free radical scavenging enzymes.MethodsTwenty...Full Text Available

208

Effect of Surgical Alteration of the Rat Gastrointestinal Tract On the Growth and Development of Hymenolepis Diminuta

Neonatal exposure to diethylstilbestrol (DES) can alter the structure of the testicular excurrent ducts in rats. We characterized these changes according to dose and time posttreatment and established...Full Text Available

1999-05-01

209

DosT and DevS are oxygen-switched kinases in Mycobacterium tuberculosis

... Gastrointestinal Tract On the Growth and Development of Hymenolepis DiminutaKerry L. Dwinell, Paul Bass, Gordon L. Telford, ... central (CNS) nervous systems in the development of Hymenolepis diminuta usi...

210

Determination of the thickness and density of the ion bombardment induced altered layer in SiC by means of reflection electron energy loss study

Exposure of Mycobacterium tuberculosis to hypoxia is known to alter the expression of many genes, including ones thought to be involved in latency, via the transcription factor DevR...Full Text Available

2007-08-01

211

DNA ploidy, nuclear size, proliferation index and DNA-hypomethylation in ovarian cancer

The steady state surfaces of ion bombarded 3C-, 4H- and 6H-SiC samples were studied by means of reflected electron energy loss spectroscopy (REELS). The REELS exhibit a well-defined loss peak in the region of about 20 eV. The position of the maximum of the loss peak depends on the bombarding ion energy (decreasing with increasing ion energy), and on the primary electron beam energy (increasing with increasing primary energy). This behavior can be explained if we suppose that the plasmon energy in the altered layer (produced by ion bombardment) is different from that of the unaltered bulk. In this case the measured loss peak is the sum of two overlapping plasmon peaks. With modeling the system as a homogeneous altered layer and a homogeneous unaltered substrate the plasmon energy in the altered layer was derived to be 19.8 eV. The large change of the plasmon energy with respect to the bulk value of 23 eV is explained by a ...

2005-12-15

212

Cytokinin-Deficient Transgenic Arabidopsis Plants Show Multiple Developmental Alterations Indicating Opposite Functions of Cytokinins in the Regulation of Shoot and Root Meristem Activity

ObjectiveThe present study was undertaken to analyze the impact of epigenetic alterations with a main focus on nuclear area, aneuploidy, hyperploidy, and proliferation...Full Text Available

2011-04-01

213

Corticosteroid can alter antigen expression on alveolar macrophages.

Cytokinins are hormones that regulate cell division and development. As a result of a lack of specific mutants and biochemical tools, it has not been possible to study the consequences of cytokinin...Full Text Available

2003-11-01

214

Copy Number Alterations and Methylation in Ewing's Sarcoma

Normal healthy volunteers underwent broncho-alveolar lavage and the cells obtained were cultured for 24 h and 48 h, either alone or in the presence of the corticosteroid, Budesonide. Cell differentials...Full Text Available

1991-09-01

215

Construction and Application of a Zebrafish Array CGH Platform

Ewing's sarcoma is the second most common bone malignancy affecting children and young adults. The prognosis is especially poor in metastatic or relapsed disease. The cell of origin remains elusive,...Full Text Available

2011-01-01

216

Conditional deletion of Abca3 in alveolar type II cells alters surfactant homeostasis in newborn and adult mice

The zebrafish is emerging as a prominent model system for studying the genetics of human development and disease. Genetic alterations that underlie each mutant model can exist in the form of...Full Text Available

2009-02-01

217

Complexity of the microRNA repertoire revealed by next-generation sequencing

ATP-binding cassette A3 (ABCA3) is a lipid transport protein required for synthesis and storage of pulmonary surfactant in type II cells in the alveoli. Abca3 was conditionally deleted...Full Text Available

2010-05-01

218

Challenges of functional imaging research of pain in children

MicroRNAs (miRNAs) have been implicated to play key roles in normal physiological functions, and altered expression of specific miRNAs has been associated with a number of diseases. It is of great interest...Full Text Available

2010-11-01

219

Castration and training in a spatial task alter the number of immature neurons in the hippocampus of male mice

Functional imaging has revolutionized the neurosciences. In the pain field it has dramatically altered our understanding of how the brain undergoes significant functional, anatomical and chemical changes...Full Text Available

220

CERES BDS Quality Summaries - Atmospheric Science Data Center - NASA

New neurons are generated in the granule cell layer of the dentate gyrus (GCL) throughout adulthood. This process is modulated by many environmental and neurochemical factors. We previously...Full Text Available

2010-05-06

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221

Application of tumor, bacterial and parasite susceptibility assays to study immune alterations induced by environmental chemicals

Nov 18, 2010 ... Roughly speaking, the fidelity interval we quote is a "3 sigma" value. ... surface of the foremost quartz filter which is then chemically altered by ... ( CERES) scanner point accuracy using a coastline detection system", ...

222

Antioxidant Treatment Alters Peripheral Vascular Dysfunction Induced by Postnatal Glucocorticoid Therapy in Rats

Model systems to study the effects of chemicals of environmental concern on bacterial and parasitic diseases as well as the immunosurveillance and destruction of transplantable tumor cells were described...Full Text Available

1982-02-01

223

Analysis on the alterations of lens proteins by Vitex negundo in selenite cataract models

BackgroundPostnatal glucocorticoid therapy in premature infants diminishes chronic lung disease, but it also increases the risk of hypertension in adulthood. Since glucocorticoid...Full Text Available

224

An imbalance between apoptosis and proliferation contributes to follicular persistence in polycystic ovaries in rats

PurposeCataract is the leading cause of blindness and is associated with oxidative damage and protein modification in the lens. In the present study, we have employed proteomic and...Full Text Available

225

Ameliorative Potential of Quercetin Against Paracetamol-induced Oxidative Stress in Mice Blood

BackgroundCystic ovarian disease is an important cause of infertility that affects bovine, ovine, caprine and porcine species and even human beings. Alterations in the ovarian micro-environment...Full Text Available

226

Altering high temperature subterranean formation permeability

The aim of the present study was to evaluate the ameliorative potential of quercetin (QC) against paracetamol (PCM)-induced oxidative stress and biochemical alterations in mice blood. A total of 36...Full Text Available

2011-07-01

227

Altered myocardial substrate metabolism is associated with myocardial dysfunction in early diabetic cardiomyopathy in rats: studies using positron emission tomography

This patent describes a delayed acrylamide containing polymer crosslinker having stability in an aqueous solution at high temperatures. It comprises: a combination of an aldehyde and a salicylic acid derivative selected from salicylamide and acetysalicylic acid.

1991-02-19

228

Altered Brain Activation in Ventral Frontal-Striatal Regions Following a 16-week Pharmacotherapy in Unmedicated Obsessive-Compulsive Disorder

BackgroundIn vitro data suggest that changes in myocardial substrate metabolism may contribute to impaired myocardial function in diabetic cardiomyopathy (DCM)....Full Text Available

229

Alterations in the steroid hormone receptor co-chaperone FKBPL are associated with male infertility: a case-control study

Recent studies have reported that cognitive inflexibility associated with impairments in a frontal-striatal circuit and parietal region is a core cognitive deficit of obsessive-compulsive disorder (OCD)....Full Text Available

2011-05-01

230

Alteration of the chemical environment disrupts communication in a freshwater fish

BackgroundMale infertility is a common cause of reproductive failure in humans. In mice, targeted deletions of the genes coding for FKBP6 or FKBP52, members of the FK506 binding...Full Text Available

231

Alteration of installation of reactors (alteration of No. 1 and No. 2 reactor facilities) in the Sendai Nuclear Power Station, Kyushu Electric Power Co. , Inc

It is well established that changes to the chemical environment can impair development, physiology and reproductive biology; by contrast, impacts on communication have not been widely reported. This...Full Text Available

2006-05-22

232

Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome

The Nuclear Safety Commission presented the report to the Minister of International Trade and Industry on April 5, 1984, after the careful investigation and deliberation on the alteration of installation of No.1 and No.2 reactor facilities in the Sendai Nuclear Power Station. The technical capability of Kyushu Electric Power Co., Inc., was recognized to be adequate. It was judged that the safety after this alteration of installation of the reactor facilities can be ensured. The main items of examination were as follows. The mechanical, nuclear and thermo-hydraulic designs of 17 x 17 B-type fuel assemblies were regarded as adequate. The coexistence of A-type and B-type fuel assemblies does not cause any problem about the safety. The safety at the time of abnormal transient change and accident in the mixed fuel assembly core was confirmed. In No.2 reactor, the degree of enrichment of the fuel for replacement and the number of fuel assemblies to ...

1984-08-01

233

Addressing the Question of the Effect of RBC Storage on Clinical Outcomes: The Red Cell Storage Duration Study (RECESS) (Section 7)

Decreased cognitive performance reduces independence and quality of life for aging individuals. Healthy brain aging does not involve significant neuronal loss, but little is known about the...Full Text Available

2010-06-01

234

Acute atrial arrhythmogenicity and altered Ca²⁺ homeostasis in murine RyR2-P2328S hearts

The question of whether storage of red blood cells (RBCs) alters their capacity to deliver oxygen and affects patient outcomes remains in a state of clinical equipoise. Studies of the changes...Full Text Available

2010-08-01

235

Actin Fusion Proteins Alter the Dynamics of Mechanically Induced Cytoskeleton Rearrangement

AimsThe experiments explored for atrial arrhythmogenesis and its possible physiological background in recently developed hetero-(RyR2^+/S) and homozygotic...Full Text Available

2011-03-01

236

Acetylation of Histones and Transcription-Related Factors

Mechanical forces can regulate various functions in living cells. The cytoskeleton is a crucial element for the transduction of forces in cell-internal signals and subsequent biological responses. Accordingly,...Full Text Available

237

Abscisic Acid Inhibition of Radicle Emergence But Not Seedling Growth Is Suppressed by Sugars1

The state of chromatin (the packaging of DNA in eukaryotes) has long been recognized to have major effects on levels of gene expression, and numerous chromatin-altering strategies—including...Full Text Available

2000-06-01

238

Abscisic Acid Alters the Metabolism of Indole-3-Acetic Acid in Senescing Flowers of Cucumis melo L

Low concentrations of sugars altered the sensitivity of seed germination to inhibition by exogenous abscisic acid (ABA). Germination of wild-type and ABA-insensitive (abi) Arabidopsis...Full Text Available

2000-04-01

239

Ab binding alters gene expression in Cryptococcus neoformans and directly modulates fungal metabolism

Experiments were conducted to investigate indole-3-acetic acid (IAA) and abscisic acid (ABA) metabolism associated with postanthesis senescence of ovaries from nonpollinated muskmelon (Cucumis...Full Text Available

1990-11-01

240

ALTERATION OF THE PKC-MEDIATED SIGNALING PATHWAY FOR SMOOTH MUSCLE CONTRACTION IN OBSTRUCTION-INDUCED HYPERTROPHY OF THE URINARY BLADDER

Abs facilitate humoral immunity via the classical mechanisms of opsonization, complement activation, Ab-dependent cellular cytotoxicity, and toxin/viral neutralization. There is also evidence that some...Full Text Available

2010-04-01

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241

AICAR inhibits adipocyte differentiation in 3T3L1 and restores metabolic alterations in diet-induced obesity mice model

Normal urinary bladder function requires contraction and relaxation of the detrusor smooth muscle (DSM). The DSM undergoes compensatory hypertrophy in response to partial bladder outlet obstruction...Full Text Available

2009-07-01

242

A rare case of neuroleptic malignant syndrome presenting with serious hyperthermia treated with a non-invasive cooling device: a case report

BackgroundObesity is one of the principal causative factors involved in the development of metabolic syndrome. AMP-activated protein kinase (AMPK) is an energy sensor that regulates...Full Text Available

243

A decrease in retinal progenitor cells is associated with early features of diabetic retinopathy in a model that combines diabetes and hypertension

IntroductionA rare side effect of antipsychotic medication is neuroleptic malignant syndrome, mainly characterized by hyperthermia, altered mental state, haemodynamic dysregulation,...Full Text Available

244

A conserved switch in sensory processing prepares developing neocortex for vision

PurposeHyperglycemia and hypertension contribute to the development of diabetic retinopathy, and this may involve alterations in the normal retinal cell cycle. In this work, we examined...Full Text Available

245

Recent advances in the neurobiology of anxiety disorders: Implications for novel therapeutics

Developing cortex generates endogenous activity that modulates the formation of functional units, but how this activity is altered to support mature function is poorly understood. Using recordings...Full Text Available

2010-08-12

246

British Library Electronic Table of Contents (United Kingdom)

Anxiety disorders are a highly prevalent and disabling class of psychiatric disorders. This review focuses on new directions in neurobiological research and implications for the development of novel psychopharmacological treatments. Neuroanatomical and neuroimaging research in anxiety disorders has centered on the role of the amygdala, reciprocal connections between the amygdala and the prefrontal cortex, and, most recently, alterations in interoceptive processing by the anterior insula. Anxiety disorders are characterized by alterations in a diverse range of neurochemical systems, suggesting ample novel targets for drug therapies. Corticotropin-releasing factor (CRF) concentrations are elevated in a subset of anxiety disorders, which suggests the potential utility of CRF receptor antagoni...

2008-01-01

247

Macro and micro observations on mortar alternation mechanism under the various solution conditions

Wiley::The Wiley Encyclopedia of Packaging Technology, 3rd Edition

Accelerated aging tests have been conducted to evaluate the long-term durability of cementitious material against aggressive ions. In tests, cementitious specimens were immersed in the solutions containing concentrated aggressive ions at high temperature and it promoted diffusion of the ions in the specimen. This method would be suitable for the evaluation on the aging as the alteration of the specimen would be expected to resemble the natural behavior. This paper presents a classification of alteration mechanism in the immersion tests using MgCl{sub 2} and Na{sub 2}SO{sub 4} solution. This classification was done by relating the changes of compressive strength to microscopic and mineralogical changes.

1995-12-31

248

Wastenet

...The+Wiley+Encyclopedia+of+Packaging+Technology%2C+3rd+Edition FO21+Food+Packaging Wiley::The Wiley Encyclopedia of Packaging Technology, 3rd Edition WILEY ...US | HELP Home / Chemistry / Food Science & Technology / Food Processing, Production & Manufacture / Food Packaging / The Wiley Encyclopedia of Packaging Technology, 3rd Edition ...Manufacture Sensory Science Food Biotechnology Food Chemistry Food Engineering Related Titles Food Packaging Brewing Yeast and Fermentation by Chris Boulton, David Quain Food ...Editor) Journal of Food Process Engineering Journal of Food Processing and Preservation Packaging Research in Food Product Design and Development by Howard R. ...

249

The antimicrobial efficacy of a silver alginate dressing against a broad spectrum of clinically relevant wound isolates

British Library Electronic Table of Contents (United Kingdom)

Wound dressings impregnated with silver have a role to play in aiding to reduce both the dressing and wound microbial bioburden. It is therefore imperative that antimicrobial wound dressings have efficacy on a broad range of clinical significant microorganisms. Accordingly, this study aimed to determine the antimicrobial efficacy of a silver alginate dressing against 115 wound isolates that had been isolated routinely from patients at West Virginia University Hospital. Standardised corrected zones of inhibition (CZOIs) were performed on all clinical isolates. It was found that the silver alginate dressing was able to inhibit the growth of all microorganisms tested. In particular, the silver alginate dressing inhibited the growth of Candida albicans and yeasts with CZOI of 3-115 mm. All met...

2011-01-01

250

The DFNA5 gene, responsible for hearing loss and involved in cancer, encodes a novel apoptosis-inducing protein

British Library Electronic Table of Contents (United Kingdom)

DFNA5 was first identified as a gene causing autosomal dominant hearing loss (HL). Different mutations have been found, all exerting a highly specific gain-of-function effect, in which skipping of exon 8 causes the HL. Later reports revealed the involvement of the gene in different types of cancer. Epigenetic silencing of DFNA5 in a large percentage of gastric, colorectal and breast tumors and p53-dependent transcriptional activity have been reported, concluding that DFNA5 acts as a tumor suppressor gene in different frequent types of cancer. Despite these data, the molecular function of DFNA5 has not been investigated properly. Previous transfection studies with mutant DFNA5 in yeast and in mammalian cells showed a toxic effect of the mutant protein, which was not seen after transfection ...

2011-01-01

251

Synthesis and study of the antifungal activity of new mono- and disubstituted derivatives of a genetically engineered polyene antibiotic 28,29-didehydronystatin A1 (S44HP)

British Library Electronic Table of Contents (United Kingdom)

Mono- and disubstituted novel derivatives of the heptaene nystatin analog 28,29-didehydronystatin A1 (S44HP, 1) were obtained by chemical modification of the exocyclic C-16 carboxyl and/or an amino group of mycosamine moiety. The strategy of preparation of mono- and double-modified polyene macrolides was based on the use of intermediate hydrophobic N-Fmoc (9-fluorenylmethoxycarbonyl) derivatives that facilitated the procedures of isolation and purification of new compounds. The antifungal activity of the new derivatives was first tested in vitro against yeasts and filamentous fungi, allowing the selection of the most active compounds that were subsequently tested for acute toxicity in mice. 2-(N,N-dimethylamino)ethylamide of 1 (2) and 2-(N,N-dimethylamino)ethylamide of N-fructopyranosyl-28...

2010-01-01

252

Loss of cell components during rehydration of dried Rhodotorula glutinis and its implications for lead uptake

British Library Electronic Table of Contents (United Kingdom)

Abstract Microbial cells are routinely dried and ground before they are used in metal biosorption studies. In this work, a metal biosorbent was prepared by drying biomass of the yeast Rhodotorula glutinis in an oven at 70C for 24-h followed by grinding. Two forms of the prepared biosorbent particles, washed and unwashed, were examined for their ability to remove lead from solution. It was found that the unwashed biosorbent exhibited higher lead uptake than the washed biosorbent. Analysis of the supernatant of washed cells incubated in water and that of unwashed cells incubated in lead solution revealed the presence of protein, carbohydrates, organic acids and inorganic phosphate. Overall, the washed and unwashed cells leached, respectively, 14.5 and 13.4% of their initial dry weight (100-m...

2011-01-01

253

In vitro effects of medium tonicity, nutrient concentration, and free chlorine content on Acanthamoeba

British Library Electronic Table of Contents (United Kingdom)

Background: The environment preferred by Acanthamoeba trophozoites and the mechanism by which the amebae enters the cornea are not yet fully understood. A better understanding of the pathogenesis of this disease may help with prevention and treatment. Purpose: To define the preferred environments for Acanthamoeba survival and proliferation in vitro by examining the effect of tonicity, nutrient concentration, and free chlorine content on Acanthamoeba. Materials and methods: Human corneal isolates of Acanthamoeba castellanii and Acanthamoeba polyphaga trophozoites were cultured at 22^oC (room temperature) in PYG (peptone-yeast extract-glucose) medium. The effect of tonicity on amebae was determined by incubating trophozoites in sodium chloride solutions in concentrations ranging from 0% to 1...

2011-01-01

254

Human type I pituitary adenylate cyclase activating polypeptide receptor (ADCYAP1R): Localization to chromosome band 7p14 and integration into the cytogenetic, physical, and genetic map of chromosome 7

Enhancement of bioleaching of a spent Ni/Mo hydroprocessing catalyst by Penicillium simplicissimum

The gene encoding the human type I pituitary adenylate cyclase activating polypeptide receptor (ADCYAP1R1) was mapped to chromosome 7 by PCR analysis of genomic DNA from a human/rodent somatic cell hybrid mapping panel. This assignment was confirmed and the gene localized to chromosome band 7p14 by fluorescence in situ hybridization. A yeast artificial chromosome containing ADCYAP1R1 was identified in the CEPH {open_quotes}B{close_quotes} Mega-YAC library. This YAC includes two highly polymorphic dinucleotide repeat sequences that will facilitate genetic studies of the contribution of ADCYAP1R1 in disease states of the central nervous and neuroendocrine systems. 13 refs., 1 fig.

1994-10-01

255

British Library Electronic Table of Contents (United Kingdom)

Statistically based experimental designs were applied to screen and optimize the bioleaching of spent hydrocracking catalyst by Penicillium simplicissimum. Eleven factors were examined for their significance on bioleaching using a Plackett-Burman factorial design. Four significant variables (pulp density, sucrose, NaNO"3, and yeast extract concentrations) were selected for the optimization studies. The combined effect of these variables on metal bioleaching was studied using a central composite design (CCD). Second-order polynomials were established to identify the relationship between the recovery percent of the metals and the four significant variables. The optimal values of the variables for maximum metals bioleaching were as follows: pulp density (4.0%, w/v), sucrose (90g/L), NaNO"3 (2...

2011-01-01

256

Curcumin Binding to DNA and RNA

British Library Electronic Table of Contents (United Kingdom)

Curcumin, the yellow pigment from the rhizoma of Curcuma longa, is a widely studied phytochemical with a variety of biological activities. The ongoing research and clinical trials have proved that this natural phenolic compound has great and diverse pharmacological potencies. Beside its effective antioxidant, antiinflammatory, and antimicrobial/antiviral properties, curcumin is also considered as a cancer chemopreventive agent. While the antioxidant activity of curcumin is well documented, its interaction with DNA and RNA is not fully investigated. This study was designed to examine the interactions of curcumin with calf thymus DNA and yeast RNA in aqueous solution at physiological conditions, using constant DNA and RNA concentration (6.25?mM) and various curcumin/polynucleotide (phosphate...

2009-01-01

257

Comparison of media and methods for detecting and enumerating Listeria monocytogenes in refrigerated cabbage.

Chromosomal localization and structure of the human type II IMP dehydrogenase gene

Direct plating, selective enrichment, and cold enrichment followed by secondary selective enrichment procedures were compared for detecting and enumerating Listeria monocytogenes in chopped cabbage stored at 5 degrees C for up to 64 days. Addition of Fe3+ to solid media enhanced detection of the organism. Cold enrichment (5 degrees C) in nutrient broth and brain heart infusion broth followed by secondary enrichment (48 h, 30 degrees C) in Trypticase soy-yeast extract-antibiotic broth and thiocyanate-nalidixic acid broth and plating on selective agar media (Doyle and Schoeni selective enrichment agar [minus acriflavin hydrochloride, supplemented with 5 micrograms of Fe3+/ml] and McBride Listeria agar) resulted in the detection of highest populations. PMID:3111369

1987-05-01

258

Biological treatment of wine of distilleries

We determined the chromosomal localization and structure of the gene encoding human type II inosine 5{prime}-monophosphate dehydrogenase (IMPDH, EC 1.1.1.205), an enzyme associated with cellular proliferation, malignant transformation, and differentiation. Using polymerase chain reaction (PCR) primers specific for type II IMPDH, we screened a panel of human-Chinese hamster cell somatic hybrids and a separate deletion panel of chromosome 3 hybrids and localized the gene to 3p21.1{yields}p24.2. Two overlapping yeast artificial chromosome clones containing the full gene for type II IMPDH were isolated and a physical map of 117 kb of human genomic DNA in this region of chromosome 3 was constructed. The gene for type II IMPDH was localized and oriented on this map and found to span no more than 12.5 kb.

1994-05-01

259

Bioconvertion of spent cellulose sausage casings

The potential of the yeast Candida tropicalis and Candida guillermondii was evaluated and an isolated partnership of microorganisms of waters of the Medellin River, conformed by two bacteria and one leavening, to degrade the content of organic matter present in wine produced by the factory of Licores and Alcoholes de Antioquia (FLA) in aerobic process with biomass production. For each one of the microorganisms in study this capacity of removal in units of chemical demand of oxygen was quantified (CDO); in addition, parameters were analyzed such as yield of the biomass in relation to the removed CDO and to total reducing sugars (TRS) consumed, time of fermentation and speed of growth different dilutions from wine. Also the possible inhibition was analyzed that the present phenolic compounds in this wine can cause in the biological process of degradation.

260

British Library Electronic Table of Contents (United Kingdom)

Cellulose sausage cellulose casings are used extensively in the manufacture of sausages in meat packaging. After stripping the meat, spent casings mainly contain cellulose and residual meat juice with salt, nitrate and nitrite. Disposal of spent sausage casings has serious economic and environmental concerns for the sausage industry. This work describes bioconversion of spent cellulose casings (SCC) into enzymes, lactic acid and ethanol by using cellulolytic fungi, lactobacillus and yeasts. The solid substrate cultivation (SSC) of Trichoderma reesei RUT C-30 on SCC and blends gave a maximum of 152 filter paper cellulase (FPase) activity and about 100 carboxymethylcellulase activity (CMCase)/g dry weight substrate. The SSC produced enzyme-rich casing with 50 FPase when directly mixed as suc...

2008-01-01

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261

Antimicrobial silver-montmorillonite nanoparticles to prolong the shelf life of fresh fruit salad

British Library Electronic Table of Contents (United Kingdom)

In this work, silver-montmorillonite (Ag-MMT) antimicrobial nanoparticles have been obtained by allowing silver ions from nitrate solutions to replace the Na^+ of natural montmorillonite and then to be reduced by a thermal treatment. Ag-MMT were used as active antimicrobial compounds to improve the shelf life of fresh fruit salad. In order to assess their influence on product shelf life, sensorial and microbiological quality has been monitored during the storage. The microbiological quality was determined by monitoring the principal spoilage microorganisms (mesophilic and psychrotrophic bacteria, coliforms, lactic acid bacteria, yeasts and molds). Additionally, the evolution of sensorial quality was assessed by monitoring color, odor, firmness and product overall quality. The Ag-MMT nanopa...

2011-01-01

262

Amphiphysin (Amph) maps to the proximal region of mouse chromosome 13

Aerobic Decolorization and Detoxification of a Disperse Dye in Textile Effluent by a New Isolate of Bacillus sp.

Amphiphysin is a protein concentrated in neuronal synapses and peripherally associated with neurotransmitter vesicles. It is expressed in many neurons of the central and peripheral nervous systems, in the adrenal medulla, in the anterior and posterior pituitary, in cell lines of the endocrine pancreas, and in spermatocytes. Its subcellular localization and tissue distribution indicate a potential involvement in mechanisms of regulated exocytosis. A role in the dynamic organization of the membrane-associated cytoskeleton is suggested by structural homology to the products of two yeast genes, RVS161 and RVS167, whose mutation results in an abnormal actin distribution, disturbs budding morphology, and impairs cell entry into stationary phase. Limited stretches of sequence similarity, including an SH3 domain, are also shared with other actin-binding proteins. Amphiphysin is the dominant autoantigen in paraneoplastic Stiff-Man syndrome, a neurological autoimmune ...

1995-07-20

263

British Library Electronic Table of Contents (United Kingdom)

A number of aerobic species capable of decolorizing some of the dyes in a textile mill effluent were isolated. One of the isolates was able to decolorize Terasil black dye under aerobic conditions in the presence of an exogenous carbon source after 5 days. Glucose or starch (%1 ea) are essential for decolorization but the process proceeds faster in the presence of 0.5% yeast extract. Results of the BOD5 show that the untreated effluent samples have a low BOD value, whereas treated samples show an initial increase in BOD up to 15 days followed by a decrease after 20 days. FT-IR and GC-MS data also reveal that the initial components in the untreated effluent disappear after 20 days of treatment, confirming biodegradation of the dye. Phytotoxicity tests on the untreated effluent samples using...

2006-01-01

264

A lytic enzyme cocktail from Streptomyces sp. B578 for the control of lactic and acetic acid bacteria in wine

British Library Electronic Table of Contents (United Kingdom)

Beside yeasts, lactic acid bacteria (LAB) are the most abundant microbes in must during vinification. Whereas Oenococcos oeni is commercially used as a starter culture for the biological acid reduction in wines, other species are responsible for different types of wine spoilage. Members of the genera Pediococcus, Weissella, Leuconostoc, and Lactobacillus are producers of exopolysaccharide slimes, biogenic amines, acetic acid, and other off-flavors. In order to control microbial growth, different procedures such as heating of must and addition of sulfite or lysozyme from egg white are generally applied. Yet, because of health risks, the application of sulfite should be reduced and lysozyme is not effective against all LAB. In this study, we describe exoenzymes from a Streptomyces sp. strain...

2009-01-01

265

Ethanol production and a case study of ethanol produced from sweet sorghum stalks via solid state fermentation

3-Methyl-3-deazaadenine, a stable isostere of N3-methyl-adenine, is efficiently bypassed by replication in vivo and by transcription in vitro.

Ethanol has excellent fuel properties, such as high octane, high heat of vaporization and low photochemical reactivity in the atmosphere. It is less volatile than gasoline and there is lower smog formation from evaporative emissions of pure ethanol compared to gasoline. As such, ethanol has emerged as an important alternative energy source that is sustainable, efficient, cost effective, convenient and safe. In 2006, global production of ethanol reached 13.5 billion gallons, up from 12.1 billion gallons in 2005. However, in light of the current debate of food versus fuel, the industry must shift to non-food feedstocks. This paper described an emerging technology to cost-effectively produce ethanol from sweet sorghum stalks, the most promising alternative feedstock to corn, via solid state fermentation (SSF). Experiments of advanced solid state fermentation (ASSF) for ethanol production from sweet sorghum by Saccharomyces cerevisiae were conducted in laboratory and pilot scales studies. ...

2008-07-01

266

System suitable for use in neutron radiography

The goal of the present work was to determine the impact of N3-methyladenine (3-mA), an important lesion generated by many environmental agents and anticancer drugs, on in vivo DNA replication and in vitro RNA transcription. Due to 3-mA chemical instability, the stable isostere 3-methyl-3-deazaadenine (3-m-c(3)A) was site specifically positioned into an oligodeoxynucleotide. The oligomer was, then incorporated into a vector system that is rapidly converted to ssDNA inside yeast cells and requires DNA replication opposite the lesion for plasmid clonal selection. For control purposes, an adenine or a stable apurinic/apyrimidinic (AP)-lesion was placed at the same site. The presence of each lesion in the oligonucleotide was confirmed by MALDI-TOF analysis. Plasmids were then transfected into yeast cells. While the AP-site dramatically reduced plasmid replication in all strains, the 3-m-c(3)A had a slight effect in the rad30 background which ...

2011-06-14

267

System suitable for use in neutron radiography

A system for real-time neutron radiography of moving objects is described. It comprise a source adapted to generate a continuous uninterrupted series of neutron pulses. Each pulse has a predetermined peak power and duration compatible with the framing times of a real-time video imaging system. A transporter moves an object to be examined to bring sequentially position selected portions of the object in the path of the pulses. Where the object itself has moving components, pulses are sequentially incident on the object. According to any dissimilarities, the object will alter the intensity of the neutrons passing therethrough. In order to record separately the changes in the neutron intensity, for each pulse, an imager is positioned to receive the neutron beam pulses which have been altered by their passage through the object and produce images indicating intensity alterations. A refrigerated beryllium filter can be used to ...

1990-02-07

268

Protracted ethanol withdrawal in rats: Tolerance to the anxiolytic effects of diazepam and pentobarbital but not phenobarbital

A system for real-time neutron radiography of moving objects is described. It comprise a source adapted to generate a continuous uninterrupted series of neutron pulses. Each pulse has a predetermined peak power and duration compatible with the framing times of a real-time video imaging system. A transporter moves an object to be examined to bring sequentially position selected portions of the object in the path of the pulses. Where the object itself has moving components, pulses are sequentially incident on the object. According to any dissimilarities, the object will alter the intensity of the neutrons passing therethrough. In order to record separately the changes in the neutron intensity, for each pulse, an imager is positioned to receive the neutron beam pulses which have been altered by their passage through the object and produce images indicating intensity alterations. A refrigerated beryllium filter can be used to ...

1988-07-20

269

Primary adenocarcinoma of the urinary bladder: value of cell cycle biomarkers.

Anxiety is a common symptom during ethanol withdrawal contributing to its continuous abuse and alcoholism. Ethanol withdrawal in rats produces an interoceptive discriminative stimulus (IDS) similar to that produced by the anxiogenic drug pentylenetetrazol (PTZ). This stimulus peaks at 12 hours after last dose of ethanol and thereafter the IDS is detected for several days (protracted withdrawal) by sensitization to a probe drug. previously, the authors have shown that during the protracted withdrawal, the IDS is enhanced by GABA receptor antagonists suggesting alteration of brain GABA systems. This report provides further evidence that chronic ethanol alters GABAergic systems. Rats were trained to discriminate PTZ (20 mg/kg, ip) from saline. Diazepam, pentobarbital and phenobarbital blocked the PTZ-IDS dose dependently. Ethanol, 4.5% w/v, was then given in a nutritionally complete diet for a week. On termination of the ethanol diet, rats ...

1990-02-26

270

Protective effect of intra-peritoneal epinephrine on postoperative renal function after cisplatin-based intra-peritoneal intra-operative chemotherapy

Primary adenocarcinomas of the urinary bladder are uncommon, and the molecular pathways are currently not well defined. In this study, we assessed the association between biologic markers and clinicopathologic characteristics in a cohort of 21 patients with primary urinary bladder adenocarcinoma. Immunohistochemical staining for cell cycle-specific markers, including p53, p21, p27, Ki-67, and cyclin E, were performed on sections of a tissue microarray construct. The tumors were high grade in 12 (57%) and pT2 or higher in 18 (86%); lymph nodes were involved in 6 cases (29%); and there was pathologic evidence of schistosomiasis in 14 (67%). The best prognostic combination of markers was combined alterations in p27 and Ki-67 and was associated with stage (P = .012), grade (P = .005), DNA ploidy (P = .005), and lymph node involvement (P = .04). Stage, lymph node involvement, combined alterations of p27 and Ki-67, and combined ...

2011-06-01

271

British Library Electronic Table of Contents (United Kingdom)

Objectives: Intra-operative cisplatin-based intra-peritoneal chemotherapy (IPC) may alter renal function in patients with advanced ovarian cancer. The aim of this study was to describe postoperative alteration of renal function after cisplatin-based IPC and to identify risk factors for moderate to severe acute renal failure. Study design: This prospective observational study was carried out on 77 consecutive patients who underwent cisplatin-based IPC procedures, with (n=23) or without (n=54) intra-peritoneal epinephrine, for advanced ovarian cancer. Postoperative renal function was assessed using serial serum creatinine measurements and was based on serum creatinine changes from pre-operative values, according to the risk, injury, failure, loss, end-stage kidney disease (RIFLE) classificat...

2011-01-01

272

Process of preparing metal parts to be heated by means of infrared radiance

Observation of the study of protein metabolism in relation to nutrition

A method for preparing metal for heating by infrared radiance to enable uniform and consistent heating. The surface of one or more metal parts, such as aluminum or aluminum alloy parts, is treated to alter the surface finish to affect the reflectivity of the surface. The surface reflectivity is evaluated, such as by taking measurements at one or more points on the surface, to determine if a desired reflectivity has been achieved. The treating and measuring are performed until the measuring indicates that the desired reflectivity has been achieved. Once the treating has altered the surface finish to achieve the desired reflectivity, the metal part may then be exposed to infrared radiance to heat the metal part to a desired temperature, and that heating will be substantially consistent throughout by virtue of the desired reflectivity.

2009-06-09

273

Influence of interphase morphology on adhesion and composite durability in semicrystalline polymer matrix composites

It is a useful hypothesis to thing of the reaction to protein deprivation as made up of two parts. First there is an initial adaptation involving rapid losses of protein. This is followed by slow continuing depletion. When considering two organ groups, such as the liver and muscle, it appears that the curve of urinary nitrogen excretion reflects the different parts they play in these two phases. The changes in the relative amounts of protein in different organs results in an alteration in the pattern of body protein at the level of the organ, the tissue and the cell. These changes are themselves the result of altered rates of synthesis and breakdown. The questions that must be asked and hopefully will be answered with the help of isotopes, are how these changes are brought about, how they are inter-related and controlled, and what is their significance? This paper contains a discussion of the few results that have been obtained so far. 1 fig.

1965-06-01

274

Impaired mitochondrial energy metabolism and kinetic properties of cytochrome oxidase following acute aluminium phosphide exposure in rat liver

The microstructure of the interphase in semicrystalline polymer matrix composites has a dramatic influence on their mechanical properties. Studies have been performed to alter this region and to correlate various interphase morphologies with changes in fiber-matrix adhesion. A reinforced nylon 66 composite, when subjected to specific thermal histories, contains an interphase composed of transcrystallinity. This region has been altered by coating fibers with a diluent, poly(vinyl pyrrolidone), and/or adding the diluent to the matrix material in very small quantities. Interphase morphology was investigated with optical microscopy, and adhesion was measured using a modified fiber pull-out test. It was found that transcrystallinity increases the interfacial shear strength. The effect different interphase morphologies have on the durability of bulk composite samples is currently under investigation.

1996-12-31

275

British Library Electronic Table of Contents (United Kingdom)

The present study was designed with an aim to analyze the effect of acute aluminium phosphide (ALP) exposure (10mg/kg b.wt, intragastrically) on the kinetic characteristics of cytochrome oxidase and energy metabolism in male Wistar rat liver mitochondria. Liver mitochondrial preparations from ALP-treated rats exhibited significant decrease (66%) in the activity of cytochrome oxidase suggesting that there was a decrease in the catalytic efficiency of the active oxidase molecules on ALP treatment. The decreased activity of cytochrome oxidase with altered NADH and succinic dehydrogenase activities might have contributed towards a significant decline in state 3 and state 4 respiration as observed. These alterations in the electron transport chain complexes in turn adversely affected the ATP sy...

2010-01-01

276

Hyperspectral remote sensing for mineral exploration in Pulang, Yunnan Province, China

British Library Electronic Table of Contents (United Kingdom)

The launch of the first spaceborne hyperspectral instrument, Hyperion, in 2000 has provoked further research into its capabilities with regard to mineral exploration. Our study in the remote, mountainous region of Pulang, China employed a two-step progressive approach, first to locate target areas characterized by hydrothermal mineral alteration, using the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER), and secondly, to attempt detailed mineral mapping using Hyperion. The preliminary target detection involved principal components and broad-band spectral analysis and led to the detection of two target areas characterized by argillic alteration, iron-oxide- and sulphate-bearing minerals. A focused hyperspectral study followed using Spectral Angle Mapper (SAM) and Mixt...

2011-01-01

277

Epigenetic regulation of Myc on retinoic acid receptor beta and PDLIM4 in RWPE1 cells

British Library Electronic Table of Contents (United Kingdom)

BACKGROUND Hypermethylation of CpG islands is a common epigenetic alteration associated with cancer. Tumor suppressor genes retinoic acid receptor beta (RARb) and PDLIM4 are hypermethylated and silenced in prostate cancer (PCa) tissues and PCa cell lines compared to normal prostate cells. METHODS In this study, a benign prostate epithelial cell line RWPE1 was used as a model to study the epigenetic regulation of Myc on the RARb and PDLIM4 promoters. Forced Myc overexpression inhibited the RARb and PDLIM4 expression. RESULTS Pyrosequencing study showed that Myc overexpression increased methylation in several CpG sites of both promoters. A DNA methylation inhibitor 5-aza-2prime-deoxycytidine reversed the epigenetic alteration effect of Myc on both RARb and PDLIM4. CONCLUSION The epigenetic r...

2009-01-01

278

Cell fate regulation by coupling mechanical cycles to biochemical signaling pathways

British Library Electronic Table of Contents (United Kingdom)

Many aspects of cellular motility and mechanics are cyclic in nature such as the extension and retraction of lamellipodia or filopodia. Inherent to the cycles of extension and retraction that test the environment is the production of mechano-chemical signals that can alter long-term cell behavior, transcription patterns, and cell fate. We are just starting to define such cycles in several aspects of cell motility, including periodic contractions, integrin cycles of binding and release as well as the normal oscillations in motile activity. Cycles of local cell contraction and release are directly coupled to cycles of stressing and releasing extracellular contacts (matrix or cells) as well as cytoplasmic mechanotransducers. Stretching can alter external physical properties or sites exposed b...

2009-01-01

279

Biological radiation effects

Biochemical alterations induced by Zn and Cd individually or in combination in the serum of Oreochromis niloticus

The stages of processes leading to radiation damage are studied, as well as, the direct and indirect mechanics of its production. The radiation effects on nucleic acid and protein macro moleculas are treated. The physical and chemical factors that modify radiosensibility are analysed, in particular the oxygen effects, the sensibilization by analogues of nitrogen bases, post-effects, chemical protection and inherent cell factors. Consideration is given to restoration processes by excision of injured fragments, the bloching of the excision restoration processes, the restoration of lesions caused by ionizing radiations and to the restoration by genetic recombination. Referring to somatic effects of radiation, the early ones and the acute syndrome of radiation are discussed. The difference of radiosensibility observed in mammalian cells and main observable alterations in tissues and organs are commented. Referring to delayed radiation effects, carcinogeneses, ...

1976-01-01

280

British Library Electronic Table of Contents (United Kingdom)

Measurement of serum biochemical parameters in response to metal exposures can be especially useful to help identify target organs of toxicity as well as the general health status of animals. Oreochromis niloticus were exposed to 5.0?mg/L?Zn, 1.0?mg/L?Cd, and 5.0?mg/L?Zn?+?1.0?mg/L?Cd mixture for 7 and 28?days, and alterations in serum enzyme activities and ion levels were measured. Significant changes in all the biochemical parameters were found to be time dependent. Following metal exposure, alkaline phosphatase activity was elevated at both exposure periods. No changes in activities of lactate dehydrogenase and lipase were observed in response to single or combined Zn and Cd exposure at 7?days while they increased at 28?days. Fish exposed to metals showed a decrease in cholinesterase ac...

2010-01-01

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281

Atrazine exposure leads to altered growth of HepG2 cells

British Library Electronic Table of Contents (United Kingdom)

Atrazine is one of the most commonly used herbicides in the United States. While effective on target plants, it has been associated with harmful health effects in non-target organisms such as fish, amphibians and mammals. In this study, growth effects on human liver cells were determined after exposure to increasing concentrations of this herbicide. Growth of immortalized human hepatoma HepG2 cells was inhibited by atrazine concentrations of 625 ppb after 72 h exposure and flow cytometry analysis demonstrated HepG2 cells exposed to 100 ppb atrazine accumulated in S phase after 48 h compared to untreated cells. Expression of cell cycle specific cyclin proteins was altered after atrazine exposure with cyclin E levels significantly decreased after a 24 h exposure and cyclin B levels decreased...

2011-01-01

282

Studies on the biosorption of uranium by a thermotolerant, ethanol-producing strain of Kluyveromyces marxianus

Potential anti fungal agents. Synthesis and activity of 2-alkylthiopyridine-4-carbothioamides

The ability of residual biomass from the thermotolerant ethanol-producing yeast strain Kluyveromyces marxianus IMB3 to function as a biosorbent for uranium has been examined. It was found that the biomass had an observed maximum biosorption capacity of 120 mg U/g dry weight of biomass. The calculated value for the biosorption maximum, obtained by fitting the data to the Langmuir model was found to be 130 mg U/g dry weight biomass. Maximum biosorption capacities were examined at a number of temperatures and both the observed and calculated values obtained for those capacities increased with increasing temperature. Decreasing the pH of the biosorbate solution resulted in a decrease in uptake capacity. When biosorption reactions were carried out using sea-water as the diluent it was found that the maximum biosorption capacity of the biomass increased significantly. Using transmission electron microscopy, uranium crystals were shown to be concentrated on the outer ...

1997-06-01

283

NADP Regulates the Yeast GAL Induction System

A series of 2-alkylthiopyrine-4-carbothioamides were synthesized, and their anti-fungal potency was tested. The chemical structures were proved by infrared spectroscopy (IR) and {sup 1}H nuclear magnetic resonance ({sup 1}H-NMR) data and by elemental analysis. The minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) assessment were used for the estimation of potential activity in vitro. The study comprising 21 clinical isolates of fungi showed that two compounds exhibited fair inhibitory activity against some yeasts and dermatophytes. Selective fungistatic activity against non-dermatophytes (MIC = 3.12-25.0 {mu}g/mL) was found also in another compound. None of the above compounds showed inhibitory activity against non-dermatophyte filamentous fungi. Microbiological activity of 2-alkylthiopyridine-4-carbothioamides appears to be mainly related to hydrophobicity of alkyl in position 2. (authors). 10 refs., 8 tabs.

1996-05-01

284

Molecular studies of the uncoupling protein

Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UASGAL); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.

2008-01-01

285

Evaluation of heavy-metal ion toxicity in fish cells using a combined stress protein and cytotoxicity assay

The uncoupling protein (UCP) is a proton/anion transporter found in the inner mitochondrial membrane of brown adipocyte. Although UCP has nor been detected in mitochondria from any other tissue, it shares structural and catalytic properties with several other mitochondrial carrier proteins. Although UCP was discovered only recently it is one of the most extensively studied mitochondrial carrier proteins.More recently, the mouse, rat, and human genes encoding for UCP have been isolated and sequenced. The availability of these various tools has led to several significant observations. UCP gene expression is strongly controlled at the level of transcription by signals that are activated after the stimulation of brown adipocytes by norepinephrine. The comparison of UCP gene with the genes encoding the adenine nucleotide translocator revealed the existence of structural and evolutionary homologies. Moreover, in humans the UCP gene and one form of adenine nucleotide translocator gene are ...

1991-06-01

286

Effects of paraquat on Escherichia coli: Differences between B and K-12 strains

All organisms, from bacteria and yeast to humans, respond to physical and chemical stressors by increasing the synthesis of a small group of cellular stress proteins.'' The authors have developed a simple in vitro system for quickly screening environmentally relevant stressors to detect stress-induced proteins that are good candidates for biomarkers. Polyacrylamide gel electrophoresis was used to detect stressor-induced, concentration-dependent changes in cellular stress protein levels in two fish cell culture systems, whereas simultaneous in vitro neutral red uptake cytotoxicity assays measured the stressors effect on cellular physiology. There was a direct concentration-dependent relationship between sublethal cytotoxic effects and the increases in stress protein levels. Increases of 50 to 200% were detected in stress proteins from desert topminnow, Poeciliopsis lucida, hepatoma-derived cell cultures exposed to cadmium or copper. Three proteins ...

1994-08-01

287

Construction of a human MluI YAC library

Escherichia coli B and K-12 are equally susceptible to the bacteriostatic effects of aerobic paraquat, but they differed strikingly when the lethality of paraquat was evaluated. E. coli B suffered an apparent loss of viability when briefly exposed to paraquat, whereas E. coli K-12 did not. This difference depended on the ability of the B-strain, but not the K-12 strain, to retain internalized paraquat; the B strain was killed on aerobic tryptic soy-yeast extract plates during the incubation which preceded the counting of colonies. This difference in retention of paraquat between strains was demonstrated by delayed loss of viability, by growth inhibition, and by cyanide-resistant respiration after brief exposure to paraquat, washing, and testing in fresh medium. This difference was also shown by using ({sup 14}C)paraquat. This previously unrecognized difference between E. coli B and K-12 has been the cause of apparently contradictory reports and should lead to some ...

1990-02-01

288

Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22

The authors describe a cloning strategy for the construction of a human genomic library in yeast artificial chromosomes (YACs) based on complete digestion of high-molecular-weight DNA with the infrequently cutting restriction enzyme MluI. Cloning of MluI fragments in the vector pYAC-RC and one subsequent size fractionation by preparative pulsed-field gel electrophoresis yielded a library with average insert sizes of 600 kb. Ninety-seven percent of the colonies were recombinant. An additional size fractionation of MluI fragments prior to ligation had no significant influence on the size of the YACs. The library currently consists of 5000 clones, which is the equivalent of one human genome. Nineteen percent of the YACs were larger than 1.2 Mb. Since smaller MluI fragments are lost during sizing, they also performed cloning without size fractionation. Only 20% of the colonies were recombinant, probably due to unligated vector fragments that were present during the ...

1994-05-01

289

Biosorption of Ni (II) by Schizosaccharomyces pombe: kinetic and thermodynamic studies

Two overlapping cDNA clones encoding human DNA topoisomerase II were identified by two independent methods. In one, a human cDNA library in phage {lambda} was screened by hybridization with a mixed oligonucleotide probe encoding a stretch of seven amino acids found in yeast and Drosophila DNA topoisomerase II; in the other, a different human cDNA library in a {lambda}gt11 expression vector was screened for the expression of antigenic determinants that are recognized by rabbit antibodies specific to human DNA topoisomerase II. The entire coding sequences of the human DNA topoisomerase II gene were determined from these and several additional clones, identified through the use of the cloned human TOP2 gene sequences as probes. Hybridization between the cloned sequences and mRNA and genomic DNA indicates that the human enzyme is encoded by a single-copy gene. The location of the gene was mapped to chromosome 17q21-22 by in situ hybridization of a cloned fragment to ...

1988-10-01

290

British Library Electronic Table of Contents (United Kingdom)

The potential of the dried yeast, wild-type Schizosaccharomyces pombe, to remove Ni(II) ion was investigated in batch mode under varying experimental conditions including pH, temperature, initial metal ion concentration and biosorbent dose. Optimum pH for biosorption was determined as 5.0. The highest equilibrium uptake of Ni(II) on S. pombe, q e, was obtained at 25??C as 33.8?mg?g?1. It decreased with increasing temperature within a range of 25?50??C denoting an exothermic behaviour. Increasing initial Ni(II) concentration up to 400?mg?L?1 also elevated equilibrium uptake. No more adsorption took place beyond 400?mg?L?1. Equilibrium data fitted better to Langmuir model rather than Freundlich model. Sips, Redlich?Peterson, and Kahn isotherm equations modelled the investigated system with a...

2011-01-01

291

A procedure for batch separation of sup 14 C-hexose from sup 14 C-sucrose

Transcription of the E2F-1 gene is rendered cell cycle dependent by E2F DNA-binding sites within its promoter.

This presentation describes a method for separating {sup 14}C-hexose from {sup 14}C-sucrose in extracts of plant tissue. Portions of ethanol extracts are treated with activated charcoal in microcentrifuge tubes. Aliquots are removed, ethanol evaporated and replaced with reaction mixture that phosphorylates hexose (HEPPS, K{sub 2}HPO{sub 4}, Mg(C{sub 2}H{sub 3}O{sub 2}){sub 2}, ovalbumen, Na{sub 2}ATP, yeast hexokinase). After a time course, the hexokinase reaction is stopped (slowed considerably) to minimize effects of contamination enzyme activities. The stopping agent used is lyxose, a nonphosphorylable analogue of glucose. The strong anionic charge of phosphate introduced through the hexokinase action results in binding (> 95%) of hexose-phosphate to anion-exchange resin. Sucrose remains unbound (> 95%) in solution. This batch ion-exchange is performed in microcentrifuge tubes to allow many samples to be processed simultaneously. Recovery of radiolabel in ...

1991-05-01

292

The Charles University in Prague Environment Centre - Environmental Economics

The cell cycle-regulatory transcription factor E2F-1 is regulated by interactions with proteins such as the retinoblastoma gene product and by cell cycle-dependent alterations in E2F-1 mRNA abundance....Full Text Available

1994-10-01

293

Wastenet

... By applying non-market valuation methods, we focus on analysing individual preferences in respect of (environmental) non-market goods. The principal object of research interest is the valuation of pollution impacts and effects of working processes on human health resulting in premature deaths, risk alterations, and various disease symptoms. We also deal with valuation of non-production ecosystem (forest) functions and benefits, inter alia ...

294

Synergistic Activation of Glucose-6-Phosphate Dehydrogenase and NAD(P)H oxidase by Src kinase Elevates Superoxide in Type 2 Diabetic, Zucker fa/fa, Rat Liver

Spectroscopic study of some historical arts

Glucose metabolism through glycolysis and hexosamine pathway has been shown to be altered in type 2 diabetes. However, its fate through the pentose phosphate pathway (PPP) is currently unclear....Full Text Available

2009-08-01

295

Silicon L/sub 2/ /sub 3/VV Auger Lineshape and oxygen chemisorption study of Pd/sub 4/Si

This work presents the spectroscopic results of the investigations of ancient pottery remains excavated in ancient Kremna (BURDUR-TURKEY). The micro-Raman and FT-IR spectroscopic studies of the pottery remains belonging to sixth century allowed us to identify the mineral composition. Some examples are described of the use of this technique in the field of art conservation and diagnostics, with regard to pigments, and some products of metal alteration.

2009-09-24

296

Shorter anogenital distance predicts poorer semen quality in young men in Rochester, New York

The Si L/sub 2/ /sub 3/VV Auger Lineshape for Pd/sub 4/Si was measured and found to be in good agreement with the self-fold of the Si partial density of states model calculated by Riley et al. Oxygen chemisorption altered both the Auger lineshape and the HeI photoemission spectrum, especially near the Fermi energy.

1981-01-01

297

DEFF Research Database (Denmark)

In male rodents, anogenital distance (AGD) provides a sensitive and continuous correlate of androgen exposure in the intrauterine environment and predicts later reproductive success. Some endocrine-disrupting chemicals can alter male reproductive tract development, including shortening AGD, in both rodents and humans. Whether AGD is related to semen quality in human is unknown.

2011-01-01

298

S179D prolactin diminishes the effects of UV light on epidermal gamma delta T cells

Radiation accidents with multi-organ failure in the United States.

Epidermal gamma delta T cells (γδ T) and Langerhans cells (LC) are immune cells altered by exposure to ultraviolet radiation (UVB), a powerful stressor resulting in immune suppression....Full Text Available

2008-01-02

299

Prenatal stress does not alter innate novelty-seeking behavioral traits, but differentially affects individual differences in neuroendocrine stress responsivity

Only a small number of radiation accidents in the United States have been severe enough to result in multi-organ failure (MOF). Medical details of selected medical misadministration and criticality cases are reviewed, with an emphasis on pathophysiology. The four criticality cases are particularly relevant for analysis of MOF, since medical treatment was supportive and did not appreciably alter the clinical evolution of radiation injury. PMID:15975871

2005-01-01

300

Polymorphisms of delta-aminolevulinic acid dehydratase (ALAD) and peptide transporter 2 (PEPT2) genes in children with low-level lead exposure

SummaryExposure to stress during prenatal or early postnatal life can dramatically impact adult behavior and neuroendocrine function. We recently began to selectively breed Sprague-Dawley...Full Text Available

2008-02-01

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301

Pitting corrosion resistance of silicon-implanted stainless steels

Low-level lead exposure during early childhood has long been associated with altered neurocognitive development and diminished cognitive functions. Over nine thousand U.S. industrial facilities...Full Text Available

2009-11-01

302

Parahydrogen clusters: Numerical estimates and physical effects

The pitting corrosion resistance of three different types of stainless steel implanted with silicon is investigated using the potentiokinetic polarization technique. The specimens are tested in 3% NaCl and 0.1 N HCl solutions. Silicon ion implantation inhibits pitting corrosion of the steels in both aqueous media. The corrosion resistance depends on the silicon dose. Post implantation annealing only slightly alters the localized corrosion. (author).

303

Overexpression of eIF-5A2 in mice causes accelerated organismal aging by increasing chromosome instability

We study by means of Quantum Monte Carlo simulations based on the Worm Algorithm the low temperature (down to T = 0.05 K) properties of parahydrogen clusters comprising up to 40 molecules. Three different intermolecular interactions are employed: the Silvera-Goldman, the Buck and the Lennard-Jones potential. Despite important discrepancies observed in the numerical estimates of energy and superfluid fraction, the mechanism by which clusters melt at low T is independent of the particular choice of the potential, whose only effect is to alter the temperature scale.

2009-02-01

304

Monocyte responses to sulfatide from Mycobacterium tuberculosis: inhibition of priming for enhanced release of superoxide, associated with increased secretion of interleukin-1 and tumor necrosis factor alpha, and altered protein phosphorylation.

BackgroundAmplification of 3q26 is one of the most frequent genetic alterations in many human malignancies. Recently, we isolated a novel oncogene eIF-5A2 within...Full Text Available

305

Method of altering the permeability of a subterranean formation

In monocytes, sulfatide, a lipid from Mycobacterium tuberculosis, blocked priming for enhanced release of superoxide (O2-) by the macrophage activating factors lipopolysaccharide, gamma interferon,...Full Text Available

1991-08-01

306

Increasing ?-3 Desaturase Expression in Tomato Results in Altered Aroma Profile and Enhanced Resistance to Cold Stress¹^[W]^[OA]

The production of water from a subterranean formation is reduced by contacting the formation with a water dispersible hydrophilic organic polymer having a molecular weight greater than 100,000 and containing carboxyl functionality and a crosslinking composition comprising water, a zirconium compound having a value of 4+, an alpha-hydroxy acid and an amine compound.

1985-06-25

307

Inbred Strain-Specific Effects of Exercise in Wild Type and Biglycan Deficient Mice

One of the drawbacks in improving the aroma properties of tomato (Solanum lycopersicum) fruit is the complexity of this organoleptic trait, with a great variety of volatiles contributing...Full Text Available

2010-06-01

308

Hydrocarbon fuels having one or more fullerenes therein as identification media

Biglycan (bgn)-deficient mice (KO) have defective osteoblasts which lead to changes in the amount and quality of bone. Altered tissue strength in C57BL6/129 (B6;129) KO mice, a property which...Full Text Available

2010-04-01

309

Feynman rules for gauge fields in the presence of a dielectric medium

An improved hydrocarbon fuel is described selected from the group consisting of gasoline, diesel fuel, kerosene, jet fuel, No. 1 heating oil, and No. 2 heating oil containing a detectable amount of one or more fullerene additives therein serving as identification means for said fuel wherein said fullerenes are present in an amount insufficient to alter any of the combustion properties of the fuel.

1993-08-10

310

Electric regulating energy produced in the Black Forest region. Elektrische Regelenergie aus dem Schwarzwald

T. D. Lee's method for developing the Feynman rules for gauge fields in a cavity is reformulated in terms of path integral. His work is then extended by deriving these rules, in the Feynman gauge, for a spherical cavity. In any gauge only the gauge particle propagator is altered, while all the Feynman rules for gauge field couplings are left unaltered by the presence of a dielectric medium.

1983-10-15

311

Effects of Short-Term Treadmill Exercise Training or Growth Hormone Supplementation on Diastolic Function and Exercise Tolerance in Old Rats

Within the Westeuropean network of the electric current supply a continuing compensation is necessary with respect to the permanent alterations of capacity and frequency. Pumped-storage plants are of considerable significance to meet these requests. The Schluchseewerk AG, Freiburg/Breisgau, has among others five pumped-storage plants in the southern part of the Black Forest. The conception and the operation of them are described. (orig.).

1991-03-01

312

Diffusion absorption heat pump. Diffusion-Absorptions-Waermepumpe

Whether the lusitropic potential of short-term exercise in aged rats is linked to an augmentation in the growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis and an alteration in the...Full Text Available

2008-09-01

313

Differential diagnosis of tumorous lesions in the iliac bone in children

The development of a gas-operated diffusion absorption heat pump for the heating of living spaces is described. By various improvement an energy efficiency of the prototypes of 1.5 was achieved. Structural alterations led to a lower overall height and lower production costs. The CFCs used in electric heat pumps were replaced by environmentally neutral ammonia. Compared with conventional gas heating systems, the CO2 output could be reduced by more than 30%. figs., tabs.

1992-02-01

314

Description and mineralogy of Tertiary volcanic ash partings and their relationship to coal seams, near Homer, Alaska

The radiologic appearance of inflammatory and tumorous lesions in the iliac bone is characterized by destructive alterations and consolidations simultaneously. This pattern is nonspecific. The value of plain films of this area is compromised by the anatomy of the iliac bone and by overlying structures. Therefore tomography, computer tomography and bone scans are necessary. Difficulties in differentiation between benign and malignant lesions are discussed and case reports are given. The importance of histologic examination is stressed.

1984-02-01

315

Dengue Plaque Reduction Neutralization Test (PRNT) in Primary and Secondary Dengue Virus Infections: How Alterations in Assay Conditions Impact Performance

Outcrops of Tertiary coal-bearing units in sea cliffs of the Kenai Peninsula provide an excellent study area for volcanic ash partings in coals. Twenty mid-to late-Miocene, 50-cm to 3-m thick coal seams exposed in the sea cliffs about 10 km west of Homer contain an average of 10 volcanic ash or lapilli tuff partings each. The bedding relationships of the coal with any one parting cannot be predicted, and the contacts of the partings with the coal range from very sharp to predominantly gradational. These bedding relationships provide clues about the surface on which the ashes fell and on which the coal was accumulating. For example, some ashes fell in standing water, others on irregular subaerial surfaces. The partings are in various stages of alteration to kaolinite and bentonite, and vary in thickness from a few millimeters to about 10 cm. The consistency and texture of the partings depend on the degree of alteration; the less ...

1985-04-01

316

CNS Expression of B7-H1 Regulates Pro-Inflammatory Cytokine Production and Alters Severity of Theiler's Virus-Induced Demyelinating Disease

Dengue virus (DENV) infection is a worsening global health problem. The plaque reduction neutralization test (PRNT) is currently considered to be the “gold standard” to characterize...Full Text Available

2009-11-01

317

Bullous pemphigoid after radiation therapy

The CNS is a unique organ due to its limited capacity for immune surveillance. As macrophages of the CNS, microglia represent a population originally known for the ability to assist neuronal stability,...Full Text Available

318

Blockade of androgen receptors is sufficient to alter the sexual differentiation of the substantia nigra pars reticulata seizure-controlling network

Electron beam therapy applied to a lymph node metastasis from a squamous cell carcinoma was followed by the development of histologically and immunologically typical bullous pemphigoid, the lesions being initially strictly confined to the irradiation area. This observation suggests that the bullous pemphigoid antigen may be altered or unmasked by electron beam radiotherapy, leading subsequently to the production of autoantibodies. The disease in this case effectively responded to the administration of tetracycline and niacinamide, a therapeutic regimen described recently.

1988-02-01

319

An example of alkalization of SiO{sub 2} in a blast furnace coke

The substantia nigra pars reticulata (SNR) controls seizures in a sex-specific manner. At postnatal day 15 (P15), SNR infusion of GABA_A receptor agonist muscimol have proconvulsant...Full Text Available

2008-03-01

320

An activated renin-angiotensin system maintains normal blood pressure in aryl hydrocarbon receptor heterozygous mice but not in null mice

Scanning electron microscopy and an electron-microprobe analysis of a sample of blast furnace (BF) coke have revealed alkalization (5.64 wt % Na{sub 2}O + K{sub 2}O) and Al saturation (17.28 wt % Al{sub 2}O{sub 3}) of SiO{sub 2} by BF gases. The K/Na{sub at} value of 1.15 in the new phase (alteration zone) reflects close atomic proportions of the elements and suggests that the abilities to incorporate K and Na during the process are almost equal. This Al saturation and alkalization of SiO{sub 2} indicates an active role for Al along with alkali metals in BF gases. The average width of the altered area in the SiO{sub 2} grain is about 10 m, which suggests that SiO{sub 2} particles of that size can be transformed fully to the new phase, provided that at least one of their faces is open to an external pore (surface of the coke) or internal pore with circulating BF gases. The grains that exceed 10 {mu}m can only be partly ...

2007-09-15

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321

Altered GABA_A receptor-mediated synaptic transmission disrupts the firing of gonadotropin-releasing hormone neurons in male mice under conditions that mimic steroid abuse

It has been postulated that fetal vascular abnormalities in aryl hydrocarbon receptor null (ahr^−/−) mice may alter cardiovascular homeostasis in adulthood....Full Text Available

2010-07-15

322

Alterations in Adenylate Kinase Activity in Human PBMCs after In Vitro Exposure to Electromagnetic Field: Comparison between Extremely Low Frequency Electromagnetic Field (ELF) and Therapeutic Application of a Musically Modulated Electromagnetic Field (TAMMEF)

Gonadotropin–releasing hormone (GnRH) neurons are the central regulators of reproduction. GABAergic transmission plays a critical role in pubertal activation of pulsatile GnRH secretion....Full Text Available

2010-05-12

323

Advancing Age Alters the Contribution of Calcium Release From Smooth Endoplasmic Reticulum Stores in Superior Cervical Ganglion Cells

This study investigated the effects of electromagnetic fields on enzymes involved in purine metabolism in human peripheral blood mononuclear cells in vitro. Cells were obtained from 20 volunteers. We...Full Text Available

2009-01-01

324

?_1A/B-Knockout mice explain the native ?_1D-adrenoceptor's role in vasoconstriction and show that its location is independent of the other ?₁-subtypes

In superior cervical ganglion (SCG) neurons calcium-induced calcium release (CICR), mediated by ryanodine receptors (RyRs), contributes to stimulation-evoked intracellular calcium ([Ca²⁺]_i)...Full Text Available

2009-01-01

325

Structural and transport properties of illitized samples from drillhole OL-KR12 in Olkiluoto. Porosity, diffusion coefficient, permeability and tomographic imaging

Background and purpose:Theoretically, three α₁-adrenoceptor subtypes can interact at the signalling level to alter vascular contraction or at the molecular level...Full Text Available

2009-12-01

326

No short-term effects of digital mobile radio telephone on the awake human electroencephalogram

Six illitized samples from drillhole OL-KR12 in Olkiluoto were analyzed by helium gas methods for their diffusion coefficient, permeability and porosity. The diffusion coefficients measured varied in the range 2.1 x 10-10 - 3.5 x 10-8 m2/s, permeabilities in the range 6 x 10-21 - 5.8 x 10-16 m2, and porosities in the range 0.2 6.2%. Variations observed in these quantities were concluded to arise from the heterogeneous structure of the sample rock. A 4 mm x 4 mm x 4 mm subsample was sawed from each of the six samples for structural analysis by x-ray microtomography. The three-dimensional structure of tomographic reconstructions, and thereby that of the samples themselves, was analyzed visually, which confirmed their heterogeneity. Samples represent altered rock whose structure and thereby transport properties vary significantly depending on the local alteration history of the rock. (orig.)

2009-10-15

327

Low-level microwave irradiations affect central cholinergic activity in the rat

A recent study reported the results of an exploratory study of alterations of the quantitative sleep profile due to the effects of a digital mobile radio telephone. Rapid eye movement (REM) was suppressed, and the spectral power density in the 8--13 Hz frequency range during REM sleep was altered. The aim of the present study was to illuminate the influence of digital mobile radio telephone on the awake electroencephalogram (EEG) of healthy subjects. For this purpose, the authors investigated 34 male subjects in a single-blind cross-over design experiment by measuring spontaneous EEGs under closed-eyes condition from scalp positions C{sub 3} and C{sub 4} and comparing the effects of an active and an inactive digital mobile radio telephone (GSM) system. During exposure of nearly 3.5 min to the 900 MHz electromagnetic field pulsed at a frequency of 217 Hz and with a pulse width of 580 {micro}s, the authors could not detect any difference in the ...

1997-05-01

328

Localized mutagenesis of the tetracycline promoter region in pBR322 by 4,5',8-trimethylpsoralen

Sodium-dependent high-affinity choline uptake was measured in various regions of the brains of rats irradiated for 45 min with either pulsed or continuous-wave low-level microwaves (2,450 MHz; power density, 1 mW/cm2; average whole-body specific absorption rate, 0.6 W/kg). Pulsed microwave irradiation (2-microseconds pulses, 500 pulses/s) decreased choline uptake in the hippocampus and frontal cortex but had no significant effect on the hypothalamus, striatum, and inferior colliculus. Pretreatment with a narcotic antagonist (naloxone or naltrexone; 1 mg/kg i.p.) blocked the effect of pulsed microwaves on hippocampal choline uptake but did not significantly alter the effect on the frontal cortex. Irradiation with continuous-wave microwaves did not significantly affect choline uptake in the hippocampus, striatum, and hypothalamus but decreased the uptake in the frontal cortex. The effect on the frontal cortex was not altered by pretreatment with ...

1987-01-01

329

Ionizing radiation alters beta-endorphin-like immunoreactivity in brain but not blood

In vitro mutagenesis of functional DNA gene fragments by covalently reactive agents permits one in principle to examine the consequent alterations in DNA sequence directly. I have carried out selective mutagenesis of the tetracycline resistance gene in the plasmid pBR322 using the long wavelength UV light activated reaction of 4,5',8-trimethylpsoralen (TMP). The mutagenized DNA sequence was the EcoR1-Hind III restriction fragment in the vicinity of the Tcsup(R) promoter. Two classes of mutants were obtained. One exhibited a high level of Tc resistance (40-60 ..mu..g/ml) but still lower than the wild-type. Interestingly, these showed no sequence alterations at all in the vicinity of the TMP-reacted fragment. The other class of mutants exhibited low levels of drug resistance (< 20 ..mu..g/ml) and two of those that were sequenced were found to contain a 15-base pair insertion to the right of the original Hind III site. Under the ...

1982-04-01

330

Effects of clove (Caryophyllus aromaticus L.) on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells

Previous behavioral and pharmacological studies have implicated endorphins in radiation-induced locomotor hyperactivity of the C57BL/6J mouse. However, the endogenous opiate(s) responsible for this behavioral change have not been identified. The present study measured beta-endorphin-like immunoreactivity (beta-END-LI) in brain, blood, and combined brain and pituitary samples from irradiated and sham-irradiated C57BL/6J mice. After radiation exposure, levels of beta-END-LI decreased significantly in the brain. A similar, but not statistically significant, decline was measured in combined brain and pituitary samples. Concentrations of blood beta-END-LI were not changed by irradiation. These radiogenic changes in beta-END-LI are in some ways similar to those observed after other stresses. However, radiation-induced locomotor hyperactivity may be mediated more by alterations of beta-END-LI in the brain than in the periphery. Other endogenous opiate systems may also ...

1983-12-01

331

Dynamic {sup 31}P-MR-spectroscopy of the quadriceps muscle. Influence of sex and age on spectroscopic results; Die dynamische 31-Phosphor-Magnetresonanz-Spektroskopie des M. quadriceps. Einfluss von Geschlecht und Alter auf spektroskopische Parameter

Clove (Caryophyllus aromaticus L.) has been used for clinical procedures. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to evaluate the effects of clove extract on the labeling blood constituents with 99mTc and on the morphology of red blood cells. Blood samples were incubated with clove, stannous chloride and 99mTc. Plasma, blood cells, insoluble fractions of plasma and blood cells were separated. The radioactivity was counted and percentage of radioactivity (%ATI) to each blood fraction was calculated. The shape and morphometric parameter (perimeter/area ratio) were evaluated. Clove extract altered significantly (p<0.05) the %ATI of blood constituents and the shape of red blood cells without modifying the perimeter/area ratio. The results indicate that clove extract presents chemical compounds that interfere with the radiolabeling of blood constituents and alter the ...

2007-09-15

332

DNA alterations photosensitized by tetracycline and some of its derivatives

Purpose: {sup 31}P-MRS is used to assess the influence of sex and age on quadriceps muscle metabolism before and after exercise. Materials and Methods: 32 healthy volunteers (15 women, 17 men; mean age: 38{+-}17 yrs.) were examined by dynamic phosphorus-31 ({sup 31}P) magnetic resonance spectroscopy (MRS). In the magnet, the quadriceps muscle was stressed by an isometric and an isotonic form of exercise until exhaustion, respectively. Results: Resting conditions: With increasing subjects` age, the ratio {beta}-adenosine triphosphate/total phosphate decreased (r=-0.37; p=0.02). With increasing subjects` age, the ratios inorganic phosphate/phosphocreatine (r=0.79; p=5x10{sup -8}), phosphomonoester/{beta}-adenosine triphosphate (r=0.74; p=10{sup -6}) and phosphodiester/{beta}-adenosine triphosphate (r=0.62; p=10{sup -4}) increased. The pH was the only one of the evaluated spectroscopic parameters which showed a sex-dependence: Female subjects had a significantly lower pH (7.03{+-}0.02) ...

1999-05-01

333

Copy number and orientation determine the susceptibility of a gene to silencing by nearby heterochromatin in Drosophila

Bacteriophage M13 mp10 DNA were irradiated with near-UV light in the presence of tetracycline derivatives and primed with synthetic oligonucleotide to be used for DNA synthesis using Escherichia coli DNA polymerase. Chain terminations were observed by denaturing polyacrylamide gel electrophoresis and mapped precisely. All the synthesis stops occurred before or at the level of guanine residues, showing that the photoreaction mediated by tetracycline derivatives led to a preferential alteration of guanine residues. These lesions were demonstrated to be induced in DNA through a pathway involving singlet oxygen. Tetracycline derivatives also photoinduced the breakage of the DNA sugar-phosphate backbone monitored by the conversion of supercoiled phi X174 DNA to a relaxed form. This lesion was shown to be initiated by hydroxyl radicals. The production of this free radical has been confirmed by electron paramagnetic resonance (EPR) spin trapping experiments using ...

1986-06-01

334

Chromosome mapping of human CDC25A and CDC25B phosphatases

The classical phenomenon of position-effect variegation (PEV) is the mosaic expression that occurs when a chromosomal rearrangements moves a euchromatic gene near heterochromatin. A striking feature of this phenomenon is that genes far away from the junction with heterochromatin can be affected, as if the heterochromatic state {open_quotes}spreads.{close_quotes} We have investigated classical PEV of a Drosophila brown transgene affected by a heterochromatic junction {approximately} 60 kb away. PEV was enhanced when the transgene was locally duplicated using P transposase. Successive rounds of P transpose mutagenesis and phenotypic selection produced a series of PEV alleles with differences in phenotype that depended on transgene copy number and orientation. As for other examples of classical PEV, nearby heterochromatin was required for gene silencing. Modifications of classical PEV by alterations at a single site are unexpected, and these observations contradict ...

1996-02-01

335

Alteration of the enterohepatic recirculation of bile acids in rats after exposure to ionizing radiation

The human CDC25 tyrosine phosphatases trigger activation of CDC2 by removing inhibitory phosphates; thus the genes encoding these phosphatases may be suspected as potential oncogenes due to their role in promoting cell division. To date, three human CDC25 genes have been identified: CDC25A, B, and C. This communication describes the mapping of CDC25A to chromosome 3p21 and CDC25B to chromosome 20p13 by fluorescence in situ hybridization with confirmation by the polymerase chain reaction of hamster-human somatic cell hybrid DNA. 3p21 is near an area frequently involved in karyotypic abnormalities in renal carcinomas, small cell carcinomas of the lung, and benign tumors of the salivary gland. 20p13 does not seem to be a common area for karyotypic alteration in tumors. Mapping of these genes to their chromosomal loci may help identify tumors with abnormal regulation of CDC25 genes due to genomic alterations. 15 refs., 3 figs.

1993-10-01

336

Alteration of alpha 1 Na+,K(+)-ATPase "8"6Rb"+ influx by a single amino acid substitution

The aim of this work was to study acute alterations of the enterohepatic recirculation (EHR) of bile acids 3 days after an 8-Gy radiation exposure in vivo in the rat by a washout technique. Using this technique in association with HPLC analysis, the EHR of the major individual bile acids was determined in control and irradiated animals. Ex vivo ileal taurocholate absorption was also studied in Ussing chambers. Major hepatic enzyme activities involved in bile acid synthesis were also measured. Measurements of bile acid intestinal content and intestinal absorption efficiency calculation from washout showed reduced intestinal absorption with significant differences from one bile acid to another: absorption of taurocholate and tauromuricholate was decreased, whereas absorption of the more hydrophobic taurochenodeoxycholate was increased, suggesting that intestinal passive diffusion was enhanced, whereas ileal active transport might be reduced. Basal hepatic secretion ...

2004-02-01

337

`Ecological forestry' and eucalypt forests managed for wood production in south-western Australia

The sodium- and potassium-dependent adenosine triphosphatase (Na+,K(+)-ATPase) maintains the transmembrane Na+ gradient to which is coupled all active cellular transport systems. The R and S alleles of the gene encoding the Na+,K(+)-ATPase alpha 1 subunit isoform were identified in Dahl salt-resistant (DR) and Dahl salt-sensitive (DS) rats, respectively. Characterization of the S allele-specific Na+,K(+)-ATPase alpha 1 complementary DNA identified a leucine substitution of glutamine at position 276. This mutation alters the hydropathy profile of a region in proximity to T3(Na), the trypsin-sensitive site that is only detected in the presence of Na+. This mutation causes a decrease in the rubidium-86 influx of S allele-specific sodium pumps, thus marking a domain in the Na+,K(+)-ATPase alpha subunit important for K+ transport, and supporting the hypothesis of a putative role of these pumps in hypertension.

338

British Library Electronic Table of Contents (United Kingdom)

The model of `ecological forestry' has evolved as a part of the development of the concept of ecosystem management. `Ecological forestry' emphasises that manipulation of a forest ecosystem should consider, and as far as practicable work within the limits of, natural disturbance patterns prior to extensive human alteration of the landscape. This paper evaluates the extent to which forest management practices in jarrah (Eucalyptus marginata) and karri (Eucalyptus diversicolor) forests of south-western Australia align with this view of the characteristics and appropriate silviculture of `ecological forestry'. Characteristics and appropriate silviculture of `ecological forestry' are evaluated in relation to (i) the stand level decisions of stand structure and harvest timing and (ii) the landsc...

2007-01-01

339

Various clinical situations and their influence on linear occlusion in treating combination syndrome: a discussion of treatment options.

The role of lesions of DNA in senescence of seeds of Lupinus polyphyllus L. induced by chronic low-intensity irradiation

Eliminating anterior tooth contact is paramount when treating combination syndrome. It is possible to do so through the use of linear occlusion with a non-interceptive arrangement and the bilateral fulcrum of protrusive stability. The presence of mandibular anterior teeth often complicates this procedure. When establishing the horizontal plane of occlusion, clinical circumstances often necessitate modifications; nevertheless, certain principles must be incorporated into the design of the prostheses. Clinical circumstances may dictate altering the approach but these principles must be maintained to eliminate anterior hyperfunction. PMID:15055634

340

The influence of scattered radiation on recording systems and quality-assurance test parameters

A nonlinear relationship between the time of accelerated aging of Lupine seeds and the indices of its survival as well as the single-strand DNA amount in cells from these seeds is established. The character of this relationship is essentially altered in chronically irradiated lupine seeds from the Chernobyl NPP exclusion zone and seems more complicated. The possible role of repair systems in these effects is discussed. The fact that chronic irradiation in low doses can modify the course of senescence in lupine seeds reflects its high biological efficiency comparing with acute irradiation.

2000-08-01

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341

The influence of scattered radiation on recording systems and quality-assurance test parameters

Scattered radiation generated in patient and imaging system has to be considered when quality-assurance tests involve dose detection or image-quality estimations. Measurement of automatic-exposure control dose can be altered by backscattering from intensifying screens of more than 10% and the equipment transmission factor can be overestimated up to a factor of 10 when only primary radiation is used. The sensitivity of intensifying screens depends on the angle of incidence of the radiation and so primary and scattered radiation are detected differently. The quality-control aspects of anti-scatter grids are discussed. (author).

1988-02-23

342

String theory, black holes, and SL(2,R) current algebra

Scattered radiation generated in patient and imaging system has to be considered when quality-assurance tests involve dose detection or image-quality estimations. Measurement of automatic-exposure control dose can be altered by backscattering from intensifying screens of more than 10% and the equipment transmission factor can be overestimated up to a factor of 10 when only primary radiation is used. The sensitivity of intensifying screens depends on the angle of incidence of the radiation and so primary and scattered radiation are detected differently. The quality-control aspects of anti-scatter grids are discussed. (author).

343

Strained silicon for quantum computing

We analyse in detail the SL(2, R) black hole by extending standard techniques of Kac-Moody current algebra to the non-compact case. We construct the elements of the ground ring and exhibit W_#infinity# type structure in the fusion algebra of the discrete states. As a consequence, we can identify some of the exactly marginal deformations of the black hole. We show that these deformations alter not only the spacetime metric but also turn on non-trivial backgrounds for the tachyon and all of the massive modes of the string. (orig.).

1993-05-01

344

Spin-orbit splittings in the relativistic mean-field theory

Strains in multivalley semiconductors can destroy the strict equivalence of the valleys that is demanded by cubic symmetry. Significant changes in the properties of a semiconductor may result. A proposed implementation of quantum computing with donor atoms in silicon would suffer from alterations of the donor wave functions caused by strains that are produced by fabrication processes. Deliberately straining the silicon to an extent that removed all but one valley from participation in the lowest donor state, would prevent further changes in the wave function by strain. The strain required can be achieved with established technology for depositing silicon on SiGe alloys. (author)

2002-03-07

345

Proceedings of the Indo-British workshop on remote sensing of environment in mining field

We have investigated the effect of the rho tensor coupling on binding energies, matter root-mean-square radii and spin orbit splittings of Ca isotopes in the relativistic mean-field theory with sigma, omega, and rho mesons. It is shown that binding energies and matter root-mean-square radii are insensitive to an alteration in the strength of the rho tensor coupling and an explanation of this is given. We have further shown that inclusion of the rho tensor coupling will give isospin-dependent spin-orbit splittings and this will greatly affect spin-orbit splittings of nuclei near the neutron drip line. (author). Letter-to-the-editor.

1995-11-01

346

Problems and approach to geological disposal of radioactive waste

16 papers are presented covering: remote sensing - an aid to environment management; remote sensing application; monitoring environmental changes over iron ore mining areas; monitoring and management of environmental degradation due to geological factors; use of Landsat multispectral scanner data in environmental monitoring; geotechnical engineering problems with abandoned limestone mines; photogrammetric monitoring of mining environment; slope monitoring in open cast mines using close range remote sensing method; geobotanical remote sensing and mineral prospecting; geographical information in study of geo-environment through remote sensing; and alteration mapping in cuprite mining district.

1993-01-01

347

PET/CT cardiology: an area whose boundaries are still out of sight

This feature articles described a concept and technical problems of geological disposal of high-level radioactive waste in the civil engineering. It consists of six papers such as the present statues and subjects of geological disposal by KITAYAMA Kazumi, the diastrophism, igneous activity, and upheaval and erosion by YAMAZAKI Haruo, the groundwater flow and evaluation of nuclear transfer by IJIRI Yuji, evaluation of alteration of cement materials in the ultra-long period by HAGA Kazuko, The Mizunami Underground Research Laboratory in course of construction by SAKAMAKI Masanori, and interview of the ninetieth president of JSCE (Japan Society of Civil Engineers), he places his hope on JSCE and civil engineers by KISHI Kiyoshi. (S.Y.)

2006-11-01

348

Oncogenic transformation produced by agents and modalities used in cancer therapy and its modulation

Positron emission tomography (PET) and X-ray computed tomography (CT) performed with PET/CT cameras allow us to obtain concurrently information on the presence and degree of alterations of myocardial perfusion and metabolism and on coronary arteries calcification. Furthermore, by gated myocardial perfusion studies, PET may provide crucial information on regional coronary blood flow reserve and endothelial dysfunction. A number of recent papers provide some insight on the potential of PET/CT in cardiology and in the assessment of various cardiovascular diseases including various types of vasculitis and metabolic diseases.

2006-05-01

349

Omic Approaches in Environmental Issues

The long-term survival of certain patients after treatment with radiation or chemotherapeutic agents has allowed the realization that agents that effectively control cancer in the human subject may also possess an oncogenic potential, resulting in secondary malignancies in a significant proportion of surviving patients. A quantitative assessment of the oncogenic effects of these agents at a cellular level is important, as is information on conditions and agents that may effectively alter the development of the neoplastic state. Cell culture systems where the neoplastic transformation of cells can be scored after exposure to carcinogens offer powerful tools for evaluating the oncogenic potential of radiation and chemotherapeutic agents.

1982-01-01

350

British Library Electronic Table of Contents (United Kingdom)

Biomonitoring requires the application of batteries of different biomarkers, as environmental contaminants induce multiple responses in organisms that are not necessarily correlated. Omic technologies were proposed as an alternative to conventional biomarkers since these techniques quantitatively monitor many biological molecules in a high-throughput manner and thus provide a general appraisal of biological responses altered by exposure to contaminants. As the studies using omic technologies increase, it is becoming clear that any single omic approach may not be sufficient to characterize the complexity of ecosystems. This work aims to provide a preliminary working scheme for the use of combined transcriptomic and proteomic methodologies in environmental biomonitoring. There are difficulti...

2011-01-01

351

Neutron star evolution with internal heating

Neuroinflammation and brain infections: Historical context and current perspectives

The thermal evolution predicted by current models of the superfluid-crust interaction is noted to differ substantially from the thermal evolution predicted by models without internal heating as well as previous models of heating. Heating rates approaching the maximum predicted by current models enhance the photon luminosity of the star in the neutrino cooling era, and dramatically alter the thermal evolution in the photon cooling era. Standard cooling models are consistent with current pulsar temperature estimates and upper limits, except those for the Vela pulsar, which are lower than predicted. 77 refs.

1989-11-01

352

British Library Electronic Table of Contents (United Kingdom)

An overview of current concepts on neuroinflammation and on the dialogue between neurons and non-neuronal cells in three important infections of the central nervous systems (rabies, cerebral malaria, and human African trypanosomiasis or sleeping sickness) is here presented. Large numbers of cases affected by these diseases are currently reported. In the context of an issue dedicated to Camillo Golgi, historical notes on seminal discoveries on these diseases are also presented. Neuroinflammation is currently closely associated with pathogenetic mechanisms of chronic neurodegenerative diseases. Neuroinflammatory signaling in brain infections is instead relatively neglected in the neuroscience community, despite the fact that the above infections provide paradigmatic examples of alterations o...

2011-01-01

353

Microbiological Transformations of Radionuclides in the Subsurface

Interacting CO2 and O3 effects on litter production, chemistry and decomposition in an aggrading northern forest ecosystem: final report

Microorganisms are ubiquitous in subsurface environments although their populations sizes and metabolic activities can vary considerably depending on energy and nutrient inputs. As a result of their metabolic activities and the chemical properties of their cell surfaces and the exopolymers they produce, microorganisms can directly or indirectly facilitate the biotransformation of radionuclides, thus altering their solubility and overall fate and transport in the environment. Although biosorption to cell surfaces and exopolymers can be an important factor modifying the solubility of some radionuclides under specific conditions, oxidation state is often considered the single most important factor controlling their speciation and, therefore, environmental behavior.

2010-01-04

354

Influence of a combination of two tetrachlorobiphenyl congeners (PCB 47; PCB 77) on thyroid status, choline acetyltransferase (ChAT) activity, and short- and long-term memory in 30-day-old Sprague-Dawley rats

The overall purpose of this research was to evaluate the independent and interactive effects of elevated levels of CO{sub 2} and O{sub 3} on tree leaf litter quality and decomposition. This research was conducted at the Aspen FACE (Free Air CO{sub 2} Enrichment) facility near Rhinelander, Wisconsin. This research comprised one facet of a larger project assessing how CO{sub 2} and O{sub 3} pollutants will alter carbon sequestration and nutrient cycling in north temperate forest ecosystems.

2004-08-03

355

In vivo recovery of glycogen metabolism in hemolymph and tissues of a freshwater field crab Barytelphusa guerini on exposure to hexavalent chromium

The important role of thyroid hormones in growth and development, maintenance of body temperature, digestion, cardiac function, and normal brain development can be disrupted by environmental contaminants like polychlorinated biphenyls (PCB). Polychlorinated biphenyls are environmental contaminants that are widespread, persistent, lipophilic, and bioaccumulate through food webs, concentrating in adipose tissue. Placental and lactational PCB exposure of offspring causes metabolic and endocrine disruptions including hypothyroxinemia, spatial learning and memory deficits, neurochemical and neurobehavioral alterations, and reproductive problems. Previous studies in our lab using the individual congeners PCB 47 (2,2',4,4'-tetrachlorobiphenyl, ortho-substituted) and PCB 77 (3,3',4,4'-tetrachlorobiphenyl, non-ortho-substituted) have demonstrated alterations in thyroid hormone levels, alterations in brain choline acetyltransferase ...

2004-10-15

356

Fluoride induced changes in protein metabolism in the tissues of freshwater crab Barytelphusa guerini.

The in vivo toxic effects of hexavalent chromium (20 mg/liter) on hemolymph glucose, tissue glycogen, total free sugars, and active and total phosphorylases of an edible, freshwater crab Barytelphusa guerini were studied. In a 15-day exposure span followed by a 15-day postexposure recovery, the time-course alterations in these constituent segments of the glycogen metabolism indicate an inconsistent depletion in metabolite levels and elevated enzyme activities during exposure period as well as hyperglycemia. An insignificant recovery was observed in these parameters on the 15th day of the postexposure phase.

1990-08-01

357

Experiments to investigate the effects of radiative cooling on plasma jet collimation

Exposure of the freshwater field crab Barytelphusa guerini to a sublethal concentration of NaF (30 mg litre(-1)) caused significant alterations in protein metabolism. After an exposure time of 15 days, the crab was found to have a marked depletion of total protein and free amino acid content. A significant elevation in transaminases, Aspartate aminotransferase (AAT) and Alanine aminotransferase (ALAT), and Glutamate dehydrogenase (GDH) activities was reflected in the free amino acid levels of the tissues. PMID:15092215

1990-01-01

358

Effects of variable hardness, ph, alkalinity, suspended clay, and humics on the chemical speciation and aquatic toxicity of copper

Preliminary experiments have been performed to investigate the effects of radiative cooling on plasma jets. Thin (3 um - 5 um) conical shells were irradiated with an intense laser, driving jets with velocities > 100 km/s. Through use of different target materials - aluminium, copper and gold - the degree of radiative losses was altered, and their importance for jet collimation investigated. A number of temporally resoved optical diagnostics was used, providing information about the jet evolution. Gold jets were seen to be narrower than those from copper targets, while aluminium targets produced the least collimated flows.

2010-01-01

359

Dipyrone-induced changes in DNA repair and other cell membrane associated processes in Escherichia coli

The effects of variable hardness, pH, alkalinity, humics, and suspended clay on the chemical speciation of copper and its toxicity to fathead minnow larvae in Lake Superior water were investigated. Two proposed methods (toxicity factors and chemical speciation) for predicting LC50 values in specific natural waters from laboratory toxicity data and the average site specific values of general water quality parameters were evaluated. The accuracy of the cupric ion-selective electrode in determining CU/sup +2/ activities in ambient and chemically altered Lake Superior water was also determined.

1986-03-01

360

Dipyrone-induced changes in DNA repair and other cell membrane associated processes in Escherichia coli

The analgesic, dipyrone (1,phenyl-2,3-dimethyl-5-pyrazolone-4-methylamino methane sulphonate sodium), at 20 mM concentration, inhibited the rejoining of single-strand scissions in DNA of Escherichia coli B/r cells induced by 20 krad gamma-radiation. The chemical altered the cell membrane structure as evidenced from the uptake of acriflavin, the efflux of potassium ions from the bacterial cells and the inhibition of alkaline phosphatase-a cell membrane associated enzyme. (author). 18 refs., 6 figures.

1982-09-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

361

Core preservation with a laminated, heat-sealed package

The analgesic, dipyrone (1,phenyl-2,3-dimethyl-5-pyrazolone-4-methylamino methane sulphonate sodium), at 20 mM concentration, inhibited the rejoining of single-strand scissions in DNA of Escherichia coli B/r cells induced by 20 krad gamma-radiation. The chemical altered the cell membrane structure as evidenced from the uptake of acriflavin, the efflux of potassium ions from the bacterial cells and the inhibition of alkaline phosphatase-a cell membrane associated enzyme. (author).

362

A new reconfiguration scheme for voltage stability enhancement of radial distribution systems

A core preservation package was developed to maintain the reservoir characteristics of core samples and consequently to improve the quality of data obtained through laboratory core analyses. The package is a heat-sealable plastic-aluminum laminate similar to those common in the food-packaging industry. The laminated core preservation package acts as an impermeable barrier to water vapor and gases, and is resistant to chemical alteration and degradation by core fluids. These performance characteristics result in effective core preservation by maintaining the fluid content of the core. Other advantages of the laminated package are that it is fast and simple to use and eliminates the cumbersome dip-coat step used in some core preservation methods.

1988-12-01

363

"1H MR spectroscopy in pediatrics

Network reconfiguration is an operation problem, which entails altering the topological structure of the distribution feeders by rearranging the status of switches in order to obtain an optimal configuration in order to minimise the system losses. This paper presents a new reconfiguration algorithm that enhances voltage stability and improves the voltage profile besides minimising losses without incurring any additional cost for installation of capacitors, tap changing transformers and related switching equipment in the distribution system. Test results on a 69 node distribution system reveal the superiority of this algorithm.

2009-09-01

364

Zonal differences in DNA synthesis activity and cytochrome P450 gene expression in livers of male F344 rats treated with five nongenotoxic carcinogens

Spectral appearance and concentrations of the most prominent metabolites are affected by brain development. This knowledge is essential for the detection of pathological changes in pediatric patients. This paper discusses specific conditions of MR spectroscopic examination of children and the effects of age on MR spectra quality and quantitation of the studied metabolites. Clinical examples show several diseases that are reflected in changes in "1H MR spectra due to pathological alterations in the biochemical pathways of the observed metabolites. Attention is given to the main metabolites such as N-acetylaspartate, creatine/phosphocreatine, cholines, lactate, inositol, etc.

2008-08-01

365

The effect of ethanol on "3"5-S-TBPS binding to mouse brain membranes in the presence of chloride

Both increased cell proliferation and {open_quotes}altered{close_quotes}CYP gene expression are prominent phenomena associated with liver tumor promotion by nongenotoxic carcinogen treatment. BRDU-labeled parenchymal nuclei were observed primarily in the periportal area of groups of rats, independent of nongenotoxic carcinogen treatment. Treatment with each of the 5 nongenotoxic carcinogens resulted in profound alterations in CPY gene expression at both the transcriptional and translational levels. Expression of CYP1A1, 1A1/2, 3A1, 2B1/2, and 4A immunoproteins demonstrated nongenotoxic carcinogen-specific patterns in both magnitude and zonal distribution. In agreement with the CYP immunoprotein data, treatment with each of the five nongenotoxic carcinogens resulted in a unique composition of mRNAs of CYP2B1, 2B2, 2C6, 2C11, 3A1, 3A2, and 4A1, which were variably increased or decreased relative to the untreated control livers, depending on the ...

1995-12-31

366

Long-Term Exposure to Ambient Fine Particulate Pollution Induces Insulin Resistance and Mitochondrial Alteration in Adipose Tissue.

The effect of in vitro and in vivo administration of ethanol on the binding of "3"5S-t-butyl-bicyclophosphorothionate ("3"5S-TBPS) to cortical brain membranes of C57B1 mice was investigated using KCl (100 mM) containing assay media. The in vitro addition of ethanol produced a dose-dependent inhibition of basal "3"5S-TBPS binding. In the presence of chloride ions, GABA and pentobarbital had a biphasic action (stimulation followed by inhibition) on "3"5S-TBPS binding, whereas diazepam only stimulated the binding. Ethanol reduced the stimulatory effects of GABA and pentobarbital in a dose-dependent manner, but had no effect on the enhancement of "3"5S-TBPS binding produced by diazepam. "3"5S-TBPS binding to cortical brain membranes was inhibited by the putative Cl"- channel blocking agent DIDS. This inhibitory action of DIDS was significantly, and dose-dependently reduced by ethanol (#<=# 100 mM ethanol). Chronic ethanol ingestion in vivo, which produced tolerance to and physical ...

367

Glutamate and the aggression neural circuit in adolescent anabolic steroid-treated Syrian hamsters (Mesocricetus auratus).

Objectives: We have previously shown that chronic exposure to ambient fine particulate matter (less than 2.5 ?m in aerodynamic diameter, PM(2.5)) pollution in conjunction with high-fat diet induces insulin resistance through alterations in inflammatory pathways. In this study we evaluated the effects of PM(2.5) exposure over a substantive duration of a rodent's lifespan and focused on the impact of long-term exposure on adipose structure and function.Methods and Results: C57BL/6 mice were exposed to PM(2.5) or filtered air (FA) (6 hours/day, 5 days/week) for duration of 10 months in Columbus, OH. At the end of the exposure, PM(2.5)-exposed mice demonstrated insulin resistance (IR) and a decrease in glucose tolerance compared with the FA-exposed group. Although there were no significant differences in circulating cytokines between PM(2.5)- and FA-exposed groups, circulating adiponectin and leptin were significantly decreased in PM(2.5)-exposed group. PM(2.5) ...

2011-08-27

368

Effect of transforming growth factor beta on synthesis of glycosaminoglycans by human lung fibroblasts

Adolescent exposure to anabolic androgenic steroids (AAS) alters the development and activity of the glutamate neural system in the latero-anterior hypothalamus (LAH) in hamsters (Mesocricetus auratus); that is, an important neural component of the adolescent AAS-induced aggressive response. In this article, we used retrograde tracing to investigate glutamate-specific alterations in the connections between the LAH and several other nuclei implicated in adolescent AAS-induced aggression. Briefly, hamsters were treated with AAS or sesame-oil control during adolescence and then microinjected with retrograde tracer into the medial amygdala (MeA), lateral septum (LS), or bed nucleus of the stria terminalis (BNST). Brains were then processed for vesicular glutamate transporter 2 (VGLUT2) and examined for AAS-induced changes in the number VGLUT2 cells containing retrograde tracer (VGLUT2/tracer) within the LAH. It is interesting to note that while ...

2011-08-22

369

Chronic treatment with polychlorinated biphenyls (PCB) during pregnancy and lactation in the rat

The processes of lung growth, injury, and repair are characterized by alterations in fibroblast synthesis and interstitial distribution of extracellular matrix components. Transforming growth factor beta (TGF-beta), which is postulated to play a role in modulating lung repair, alters the distribution of several matrix components such as collagen and fibronectin. We studied the effect of TGF-beta on the synthesis and distribution of the various glycosaminoglycans (GAGs) and whether these effects may explain its role in lung repair. Human diploid lung fibroblasts (IMR-90) were exposed to various concentrations of TGF-beta (0-5 nM) for variable periods of time (0-18 h). Newly synthesized GAGs were labeled with either (3H)glucosamine or (35S)sulfate. Individual GAGs were separated by size exclusion chromatography after serial enzymatic and chemical digestions and quantitated using scintillation counting. There was a dose-dependent increase in total ...

1990-09-01

370

Alteration of leached glass surface

The gender-specific expression pattern of aromatase and 5alpha-reductases (5alpha-R) during brain development provides neurons the right amount of estradiol and DHT to induce a dimorphic organization of the structure. Polychlorinated biphenyls (PCBs) are endocrine disruptive pollutants; exposure to PCBs through placental transfer and breast-feeding may adversely affect the organizational action of sex steroid, resulting in long-term alteration of reproductive neuroendocrinology. The study was aimed at: a) evaluating the hypothalamic expression of aromatase, 5alpha-R1 and 5alpha-R2 in fetuses (GD20), infant (PN12), weaning (PN21) and young adult (PN60) male and female rats exposed to PCBs during development; b) correlating these parameters with the time of testicular descent, puberty onset, estrous cyclicity and copulatory behavior; c) evaluating possible alterations of some non reproductive behaviors (locomotion, learning and memory, ...

2009-08-15

371

Yeast allosteric chorismate mutase is locked in the activated state by a single amino acid substitution

The leached glass is subject to leachant attack and results in surface layer alteration which greatly affects the corrosion progress of glass forms. This work studied the composition change, precipitation, pitting corrosion as well as surface layer break and spallation of the leached glass forms. 6 conclusions can be made: 1. The extent of surface layer alteration is related to many factors such as temperature, pH, leachant chemistry, flow rate and leach duration, etc. 2. The alkali element Na is seriously depleted and the elements like U.Ti,Fe, Ca and Mg are enriched in the surface layer. The influence of media has such order: Fe{sub 2}O{sub 3} > Na-bentonite > Zeolite. 3. The precipitate formed in leach test for 56 days at 90 {infinity}C is mostly amorphous, but after 91 days has crystallized. There is K - Ca - Al - Si crystal,the amount of Al, K and Si is increasing, but the amount of Ca is decreasing along with leaching time. ...

1997-07-01

372

The effect of pulse voltage and capacitance on biosorption of uranium by biomass derived from whiskey distillery spent wash

Chorismate mutase, a branch-point enzyme in the aromatic amino acid pathway of Saccharomyces cerevisiae, and also a mutant chorismate mutase with a single amino acid substitution in the C-terminal part of the protein have been purified approximately 20-fold and 64-fold from overproducing strains, respectively. The wild-type enzyme is activated by tryptophan and subject to feedback inhibition by tyrosine, whereas the mutant enzyme does not respond to activation by tryptophan nor inhibition by tyrosine. Both enzymes are dimers consisting of two identical subunits of M_r 30,000, each one capable of binding one substrate and one activator molecule. Each subunit of the wild-type enzyme also binds one inhibitor molecule, whereas the mutant enzyme lost this ability. The enzyme reaction was observed by "1H NMR and shows a direct and irreversible conversion of chorismate to prephenate without the accumulation of any enzyme-free intermediates. The kinetic data of the wild-type chorismate mutase ...

373

Quality changes of irradiated spices stored in different indigenous packaging materials

Biosorption of uranium by residual biomass from The Old Bushmill`s Distillery Co. Ltd., Bushmills, Co. Antrim, Northern Ireland, following exposure to short and intense electric pulses has been examined. The biomass was prepared from the distillery spent wash and consisted of non-viable yeast and bacterial cells. As shown previously, untreated biomass had a maximum biosorption capacity of 170 mg uranium/g dry weight biomass. When biosorption reactions were placed between two electrodes and exposed to electric pulses with field strengths ranging from 1.25-3.25 kV/cm at a capacitance of 25 {mu}F, biosorption increased from 170 mg of uranium to 275 mg uranium/g dry weight biomass. The data were obtained from biosorption isotherm analyses and taken as the degree of biosorption at residual uranium concentrations of 3 mM. In addition, when the capacitance of the electric pulses increased from 0.25 {mu}F to 25 {mu}F at a fixed pulse field strength the degree of ...

1999-01-01

374

Multicolor FISH mapping with Alu-PCR-amplified YAC clone DNA determines the order of markers in the BRCA1 region on chromosome 17q12-q21

The changes in quality of irradiated spices, i.e., nutmeg (Myristica fragrans), black pepper and white pepper (Piper nigrum) stored in different indigenous packaging materials were evaluated. Packaging materials used were tin cans; tin can lined with polypropylene film, tin can lined with wrapping paper, woven polypropylene bag and woven polypropylene bag lined with polypropylene film. Packaging in woven polypropylene bag could cause significant changes in a_w-values and moisture contents of the spices. In the tin can, no significant change was found during storage. By applying a polypropylene film liner inside the plastic bag, the changes could be retarded. The other parameters observed, i.e. total plate count, total mould and yeast count and organoleptic scores of the samples were not significantly affected by the packaging method. A dose of 5 kGy could reduce the microbial load of the spices by about 2-3 log cycles. A significant decrease in a_w-value and ...

1990-03-01

375

Molecular evolution and characterization of fungal indoleamine 2,3-dioxygenases.

A gene designated BRCA1, implicated in the susceptibility to early-onset familial breast cancer, has recently been localized to chromosome 17q12-q21. To date, the order of DNA markers mapped within this region has been based on genetic linkage analysis. The authors report the use of multicolor fluorescence in situ hybridization to establish a physically based map of five polymorphic DNA markers and 10 cloned genes spanning this region. Three cosmid clones and Alu-PCR-Generated products derived from 12 yeast artificial chromosome clones representing each of these markers were used in two-color mapping experiments to determine an initial proximity of markers relative to each other on metaphase chromosomes. Interphase mapping was then employed to determine the order and orientation of closely spaced loci by direct visualization of fluorescent signals following hybridization of three probes, each detected in a different color. Statistical analysis of the combined data ...

1993-09-01

376

Functions of mammalian Cdc7 kinase in initiation/monitoring of DNA replication and development

Indoleamine 2,3-dioxygenase (IDO) and tryptophan 2,3-dioxygenase (TDO) are tryptophan-degrading enzymes. Mammalian IDO expression is induced by cytokines and has antimicrobial and immunomodulatory effects. A major role of mammalian TDO is to supply nicotinamide adenine dinucleotide (NAD(+)). In fungi, the IDO homologue is thought to be expressed constitutively and supply NAD(+), as TDO is absent from their genomes. Here, we reveal the distribution of IDO genes among fungal species and characterize their enzymatic activity. The yeast, Saccharomyces cerevisiae has only one IDO gene, whereas the koji-mold, Aspergillus oryzae has two genes, IDO? and IDO?. The A. oryzae IDO? showed more similar enzymatic properties to those of S. cerevisiae IDO than IDO?, suggesting that the A. oryzae IDO? is a functional homologue of the S. cerevisiae IDO. From the IDO? gene, two isoforms, IDO? and IDO?(+) could be generated by alternative splicing. The latter contained a 17 amino ...

2010-12-18

377

Caldicellulosiruptor obsidiansis sp. nov., an anaerobic, extremely thermophilic, cellulolytic bacterium isolated from Obsidian Pool, Yellowstone National Park

Cdc7 kinase plays an essential role in firing of replication origins by phosphorylating components of the replication complexes. Cdc7 kinase has also been implicated in S phase checkpoint signaling downstream of the ATR and Chk1 kinases. Inactivation of Cdc7 in yeast results in arrest of cell growth with 1C DNA content after completion of the ongoing DNA replication. In contrast, conditional inactivation of Cdc7 in undifferentiated mouse embryonic stem (ES) cells leads to growth arrest with rapid cessation of DNA synthesis, suggesting requirement of Cdc7 functions for continuation of ongoing DNA synthesis. Furthermore, loss of Cdc7 function induces recombinational repair (nuclear Rad51 foci) and G2/M checkpoint responses (inhibition of Cdc2 kinase). Eventually, p53 becomes highly activated and the cells undergo massive p53-dependent apoptosis. Thus, defective origin activation in mammalian cells can generate DNA replication checkpoint signals. Efficient removal of ...

2003-11-27

378

Biological assessments of a mixture of endocrine disruptors at environmentally relevant concentrations in water following UV/H{sub 2}O{sub 2} oxidation

A novel, obligately anaerobic, extremely thermophilic, cellulolytic bacterium, designated OB47T, was isolated from Obsidian Pool, Yellowstone National Park, WY, USA. The isolate was a non-motile, non-spore forming, Gram-positive rod approximately 2 m long by 0.2 m wide and grew at temperatures between 55-85oC with the optimum at 78oC. The pH range for growth was 6.0-8.0 with values of near 7.0 being optimal. Growth on cellobiose produced the fastest specific growth rates at 0.75 hr-1. The organism also displayed fermentative growth on glucose, maltose, arabinose, fructose, starch, lactose, mannose, sucrose, galactose, xylose, arabinogalactan, Avicel, xylan, filter paper, processed cardboard, pectin, dilute acid-pretreated switchgrass and Populus. OB47T was unable to grow on mannitol, fucose, lignin, Gelrite, acetate, glycerol, ribose, sorbital, carboxymethylcellulose and casein. Yeast extract stimulated growth and thiosulfate, sulfate, nitrate, and sulfur were not ...

2010-02-01

379

Y Chromosome microdeletion and altered sperm quality in human males with high concentration of seminal hexachlorocyclohexane (HCH)

Numerous studies have investigated degradation of individual endocrine disrupting compounds (EDCs) in lab or natural waters. However, natural variations in water matrices and mixtures of EDCs in the environment may confound analysis of the treatment efficiency. Because chemical based analytical methods cannot represent the combined or synergistic activities between water quality parameters and/or the EDC mixtures at environmentally relevant concentrations ({mu}g L{sup -1}-ng L{sup -1}), bioanalytical assessments of residual estrogenic activity in treated water were used to evaluate the performance of the UV based advanced oxidation process for estrogenic contaminants in water. Four EDCs including estradiol (E{sub 2}), ethinyl estradiol (EE{sub 2}), bisphenol-A (BPA) and nonylphenol (NP) were spiked individually or as a mixture at {mu}g L{sup -1}-ng L{sup -1} in laboratory or natural river water. The removal rates of estrogenic activity were quantitatively evaluated by in vitro ...

2007-04-15

380

British Library Electronic Table of Contents (United Kingdom)

Recent studies have shown Y chromosome microdeletions associated with male infertility. The factors responsible for Y chromosome microdeletions in spermatozoa remain unresolved. However, the environmental pollutants are known to damage DNA in differentiating and maturing germ cells in the male reproductive tract. Therefore, the aim of this study was to investigate the effects of seminal hexachlorocyclohexane (HCH) and its isomers, an environmental pollutant, in 50 fertile and 50 infertile males in relation to semen quality and the incidence of Y chromosome microdeletion in azoospermic factor (AZF) region. As compared to control, an increased HCH level and significantly decreased semen quality were observed in the infertile males. A positive significant association was found between sperm c...

2010-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

381

Well log interpretation of certain geothermal fields in the Imperial Valley, California

Well Test Analysis in Naturally Fissured, Geothermal Reservoirs with Fracture Skin

This study reviews the wireline log responses of some geothermal fields in the Imperial Valley, California. The fields under study include the Heber, the East Mesa, the Brawley, and the Westmoreland. The well logs used in the study did not include all the wireline surveys obtained by the operators. The selected well logs obtained under special arrangements with the operators were chosen to maintain the anonymity of specific well locations but are only representative of each area. Analysis of the well logs indicates that on an individual field basis, the well logs are excellent for correlation purposes. The presence of extremely saline fluids in some fields precludes the monitoring of Q/sub v/ (cation exchange capacity per unit volume) profile for detection of hydrothermally altered zones. The producing sections in all the fields are characterized by low porosity and high resistivity.

1984-03-01

382

Use of measured time delays in gravitational lenses to determine the hubble constant

Mineral deposition or alteration is commonly found at fracture-block interfaces is fissured, geothermal reservoirs. In response to pressure reduction in the fissures such mineralization, if less permeable than the matrix rock, will retard the flow of fluid from the blocks to the fissures and is termed fracture skin in this paper. The problem of fluid flow to a production well in a double-porosity reservoir with fracture skin was analyzed theoretically. One of the findings of the analysis was that fully transient block-to-fissure flow can be approximated by pseudo-steady state flow if fracture skin permeability is sufficiently low. Type curves generated by numerical inversion of Laplace transform solutions are used to cooroborate the results of a finite-difference model of steam transport to a well in a naturally fissured, geothermal reservoir with fracture skin.

1983-12-15

383

Uncoupling action of polychlorinated biphenyls (Kanechlor-400) on oxidative phosphorylation in rat liver mitochondria

Gravitational lenses are rare in the known samples of quasars, indicating that the conditions involved in their formation are unusual. In particular, the distribution of matter along the light rays from the observer through the deflector to the quasar may be very different from mean conditions. We show that reasonable deviations in the density of matter along the beams can significantly alter the relationship between time delays and the Hubble constant (H/sub 0/) and conclude that gravitational lenses are not promising estimators of H/sub 0/. However, should an independent, precise determination of H/sub 0/ become available, gravitational lenses could be used to probe long-range density fluctuations.

1985-04-15

384

Transforming Growth Factor-b Induces Differentiation of the Labyrinthine Trophoblast Stem Cell Line SM10

A study was made of the uncoupling action of polychlorinated biphenyls (Kanechlor-400) on oxidative phosphorylation in rat liver mitochondria. Kanechlor-400 (KC-400) at 20 ..mu..g/ml stimulated state 4 respiration of rat liver mitochondria more than 4-fold with ..cap alpha..-ketoglutarate/malate as a substrate, and released the oligomycin-inhibited state 3 respiration. KC-400 also dissipated the membrane potential across the mitochondrial membranes; thus, it acts as an uncoupler of oxidative phosphorylation in rat liver mitochondria. KC-400 altered the permeability properties of mitochondrial membranes as evidenced by the release of endogenous K/sup +/ and the oxidation of exogenously supplied NADH. It is concluded that KC-400 produces a nonspecific increase in mitochondrial ion permeability, thereby dissipating membrane potential, which leads to the uncoupling.

1984-03-01

385

British Library Electronic Table of Contents (United Kingdom)

The mammalian placenta consists of different trophoblast cell types that assist in the variety of functions required for the maintenance of pregnancy. In rodents, labyrinthine trophoblasts of the placenta are especially important, because they are capable of differentiating into fused labyrinthine cells, which form the feto-maternal exchange surface. Even though the molecular signals triggering labyrinthine trophoblast differentiation are poorly understood, transforming growth factor-b (TGF-b) has been shown to be present in the placental environment and alter trophoblast development. In this study, we investigated the effects of TGF-b on the differentiation of the labyrinthine trophoblast stem cell lines SM10 and HRP-1. RT-PCR analyses demonstrated that while the molecular expression of l...

2005-01-01

386

Thermal energy storage system with stearic acid as phase change material

The high-density regime of kinetic-dominated loop quantum cosmology

A simple tube-in-tube heat exchanger system for thermal energy storage employing stearic acid as PCM has been investigated. The performance and heat transfer characteristics of such a system were studied. Phase transition temperature range and times were measured and the speed of the phase transition front was computed. The melting front was found to move in the radial direction inward as well as in the axial direction from the top toward the bottom of the phase change material PCM tube. The speed of the melting front is enhanced by a convection heat transfer mechanism in the melted PCM. The heat transfer rate and, consequently, phase transition time can be altered by changing the water inlet temperature to the heat exchanger. In addition, a faster phase transition is realized by placing the heat exchanger in a horizontal position rather than a vertical one. (Author)

1994-10-01

387

The effects of soil type and chemical treatment on nickel speciation in refinery enriched soils: A multi-technique investigation

We study the dynamics of states perturbatively expanded about a harmonic system of loop quantum cosmology, exhibiting a bounce. In particular, the evolution equations for the first and second order moments of the system are analyzed. These moments back-react on the trajectories of the expectation values of the state and hence alter the energy density at the bounce. This analysis is performed for isotropic loop quantum cosmology coupled to a scalar field with a small but non-zero constant potential, hence in a regime in which the kinetic energy of matter dominates. Analytic restrictions on the existence of dynamical coherent states and the meaning of semi-classicality within these systems are discussed. A numerical investigation of the trajectories of states that remain semi-classical across the bounce demonstrates that, at least for such states, the bounce persists and that its properties are similar to the standard case, in which the moments of the states are ...

2010-01-01

388

British Library Electronic Table of Contents (United Kingdom)

Aerial deposition of Ni from a refinery in Port Colborne, Ontario, Canada has resulted in the enrichment of 29km2 of land with Ni concentrations exceeding the Canadian Ministry of the Environment's remedial action level of 200mgkg-1. Several studies on these soils have shown that making the soils calcareous was effective at reducing chemically extractable Ni, as well as alleviating Ni phytotoxicity symptoms in vegetable crops grown in the vicinity of the refinery. Conversely, dolomitic limestone additions resulted in increased uptake of Ni in the Ni hyperaccumulator Alyssum murale `Kotodesh', a plant whose use was proposed as a remediation strategy for this area. In this paper we use multiple techniques to directly assess the role soil type and lime treatments play in altering the speciati...

2007-01-01

389

The effect of metal ceramic restoration framework design on tooth color

British Library Electronic Table of Contents (United Kingdom)

Statement of problem The opacity of the metal framework in metal ceramic restorations results in reduced light transmission through the tooth that diminishes the esthetics of the tooth. Purpose The purpose of this study was to investigate the color change that occurs apically to a metal ceramic restoration as a result of altering the metal framework design. Material and methods Color change relative to the unprepared tooth (DE) between various metal ceramic restorations was determined using computer imaging and CIELAB coordinates and compared to previously determined levels of clinical acceptability. Color change was evaluated by examining 2 conditions (the metal copings and the completed metal ceramic restorations), 4 framework designs (metal collar and porcelain margins with 0-mm, 1-mm, ...

2008-01-01

390

Testosterone reduces amygdalaorbitofrontal cortex coupling

British Library Electronic Table of Contents (United Kingdom)

Testosterone influences various aspects of affective behavior, which is mediated by different brain regions within the emotion circuitry. Previous neuroimaging studies have demonstrated that testosterone increases neural activity in the amygdala. To investigate whether this could be due to altered regulation of amygdala functioning which is thought to be mediated by the prefrontal cortex, we studied the effects of exogenous testosterone on the interaction between the amygdala and other brain regions. Healthy middle-aged women received a single nasal testosterone dose in a randomized, placebo-controlled, crossover manner, and performed an emotional face matching task while their brain activity was measured with functional MRI. The results show that testosterone rapidly reduced functional co...

2010-01-01

391

Summing Radiative Corrections to the Effective Potential

Stress-induced alterations in anxiety-like behavior and adaptations in plasticity in the bed nucleus of the stria terminalis

When one uses the Coleman-Weinberg renormalization condition, the effective potential $V$ in the massless $\\phi_4^4$ theory with O(N) symmetry is completely determined by the renormalization group functions. It has been shown how the $(p+1)$ order renormalization group function fix the N$^{p}$LL order contribution to $V$. We discuss here how, in addition to fixing the N$^{p}$LL contribution to $V$, the $(p+1)$ order renormalization group functions also can be used to determine portions of the N$^{p+n}$LL contributions to $V$. When these contributions are summed to all orders, the singularity structure of \\mcv is altered. An alternate approach to fixing \\mcv in terms of the renormalization group functions is shown to eliminate dependence on the background field if spontaneous symmetry breaking occurs.

2010-01-01

392

British Library Electronic Table of Contents (United Kingdom)

In vulnerable individuals, exposure to stressors can result in chronic disorders such as generalized anxiety disorder (GAD), major depressive disorder (MDD), and post-traumatic stress disorder (PTSD). The extended amygdala is critically implicated in mediating acute and chronic stress responsivity and anxiety-like behaviors. The bed nucleus of the stria terminalis (BNST), a subregion of the extended amygdala, serves as a relay of corticolimbic information to the paraventricular nucleus of the hypothalamus (PVN) to directly influence the stress response. To investigate the influence of the corticosteroid milieu and housing conditions on BNST function, adult C57Bl/6J were either acutely or chronically administered corticosterone (CORT, 25mg/kg in sesame oil) or vehicle (sesame oil) or were g...

2011-01-01

393

Strategies and computational tools for improving randomized protein libraries.

Spinal axis irradiation with electrons: Measurements of attenuation by the spinal processes

In the last decade, directed evolution has become a routine approach for engineering proteins with novel or altered properties. Concurrently, a trend away from purely 'blind' randomization strategies and towards more 'semi-rational' approaches has also become apparent. In this review, we discuss ways in which structural information and predictive computational tools are playing an increasingly important role in guiding the design of randomized libraries: web servers such as ConSurf-HSSP and SCHEMA allow the prediction of sites to target for producing functional variants, while algorithms such as GLUE, PEDEL and DRIVeR are useful for estimating library completeness and diversity. In addition, we review recent methodological developments that facilitate the construction of unbiased libraries, which are inherently more diverse than biased libraries and therefore more likely to yield improved variants. PMID:16095966

2005-10-01

394

Sorbent extraction and high performance liquid chromatography (HPLC) of homidium bromide and isometamidium chloride in bovine plasma

Electrons may be used beneficially for spinal axis irradiation in medulloblastoma children to avoid some of the long-term sequelae induced by megavoltage photons. However, the attenuation by the intervening bone ought to be considered. Three-dimensional computer treatment planning with inhomogeneity correction for electron beams is not yet generally available, and alternate methods are needed to evaluate the attenuation by the complex bony structure of the spine. Here, we present our experimental data showing the alteration in the electron isodoses due to the intervening spinous processes. Film dosimetric measurements were made in the vertebral columns obtained from autopsies of a goat, a dog, and a child. Our results show that electron beam therapy for the spinal axis is a viable option.

1986-07-01

395

Solute carrier transporters as targets for drug delivery and pharmacological intervention for chemotherapy

Homidium bromide and isometamidium chloride are used extensively in the treatment of bovine trypanosomiasis in Africa, but no specific and sensitive method to detect the drug in plasma has been published yet. Comparing the methods of other investigators it was found that they could not overcome the protein binding of these drugs which reduced the available and determined drug to 10% of the real concentrations. These techniques overcome the protein binding by enzyme digestion and an alteration of the pH before adding the sample directly on clean-up columns. Drug recover rates above 80% were obtained. For the detection, the HPLC was employed using a C18 reversed phase analytical column and UV detection, determining both drugs as intact molecules. (author).

1992-01-01

396

British Library Electronic Table of Contents (United Kingdom)

Abstract Many solute carrier transporters that interact with anticancer agents and contribute to their pharmacokinetics have been shown to be differentially upregulated in cancer cells as a result of adaptive response to altered nutritional requirements. This review focuses on pathophysiological function of membrane transporters responsible for the influx of physiological substances including oligopeptides, amino acids, and organic cations and anions, and summarizes the recent knowledge regarding mechanisms in their gene expressions. Broad substrate specificity of enhanced oligopeptide H+/peptide cotransporter 1 activity in cancer cells is useful for tumor tissue-specific delivery of chemotherapeutic agents and positron emission tomography diagnostic probes. Amino acid transporters such as...

2011-01-01

397

Short-term oral administration of several manganese compounds in mice: Physiological and behavioral alterations caused by different forms of manganese

British Library Electronic Table of Contents (United Kingdom)

In Kanazawa, Japan, air pollution from a Mn treatment factory was found to affect local junior high school students, who showed reduced respiratory function relative to their exposure to Mn. The use of Mn as an antiknock ingredient in automobile fuels has recently been introduced, placing the general population at risk of chronic low-level exposure. There are few animal studies on the effects of compounds other than manganese chloride (MnCl{sub 2}). In the case of environmental pollution by Mn, the differing physiological effects of the various chemical forms of Mn must be considered. The present study therefore examined the differences in the effects of several Mn compounds on the physiology and behavior of mice by short-term oral administration.

1991-06-01

398

Abstract Capillary permeability is a tightly regulated feature of microcirculation in all organ beds. In sepsis, this feature is fundamentally altered. We have previously reported elevated levels of angiopoietin-2 in patients with septic shock, and have investigated tumor necrosis factor (TNF)-related and weak inducer of apoptosis (TWEAK), which mediates both angiogenesis and inflammation, in those patients. Enzyme-linked immunoassay was used to measure serum TWEAK levels in 20 patients with septic shock, all of whom were treated by direct hemoperfusion with a polymyxin B-immobilized fiber column (DHP-PMX), and in 20 non-septic controls. The TWEAK levels were higher in patients with septic shock (192.8--230.5-pg/mL) than in controls (84.1--28.7-pg/mL, P-=-0.043). Between 11 survivors and 1...

2011-01-01

399

Reversed light-dark cycle and cage enrichment effects on ethanol-induced deficits in motor coordination assessed in inbred mouse strains with a compact battery of refined tests

British Library Electronic Table of Contents (United Kingdom)

The laboratory environment existing outside the test situation itself can have a substantial influence on results of some behavioral tests with mice, and the extent of these influences sometimes depends on genotype. For alcohol research, the principal issue is whether genotype-related ethanol effects will themselves be altered by common variations in the lab environment or instead will be essentially the same across a wide range of lab environments. Data from 20 inbred strains were used to reduce an original battery of seven tests of alcohol intoxication to a compact battery of four tests: the balance beam and grip strength with a 1.25g/kg ethanol dose and the accelerating rotarod and open-field activation tests with 1.75g/kg. The abbreviated battery was then used to study eight inbred str...

2011-01-01

400

Responses of terrestrial ecosystems to temperature and precipitation change: a meta analysis of experimental manipulation

British Library Electronic Table of Contents (United Kingdom)

Abstract Global mean temperature is predicted to increase by 2 7 C and precipitation to change across the globe by the end of this century. To quantify climate effects on ecosystem processes, a number of climate change experiments have been established around the world in various ecosystems. Despite these efforts, general responses of terrestrial ecosystems to changes in temperature and precipitation, and especially to their combined effects, remain unclear. We used meta analysis to synthesize ecosystem level responses to warming, altered precipitation, and their combination. We focused on plant growth and ecosystem carbon (C) balance, including biomass, net primary production (NPP), respiration, net ecosystem exchange (NEE), and ecosystem photosynthesis, synthesizing results from 85 studi...

2011-01-01

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401

Research update: Alpha7 nicotinic acetylcholine receptor mechanisms in Alzheimer's disease

British Library Electronic Table of Contents (United Kingdom)

Aberrant amyloid-b peptide (Ab) accumulation along with altered expression and function of nicotinic acetylcholine receptors (nAChRs) stand prominently in the etiology of Alzheimer's disease (AD). Since the discovery that Ab is bound to a7 nAChRs under many experimental settings, including post-mortem AD brain, much effort has been expended to understand the implications of this interaction in the disease milieu. This research update will review the current literature on the a7 nAChR-Ab interaction in vitro and in vivo, the functional consequences of this interaction from sub-cellular to cognitive levels, and discuss the implications these relationships might have for AD therapies.

2011-01-01

402

Research and development for treatment and disposal technologies of TRU waste

Recycling flow control device for a nuclear reactor

After the publication of the 2nd progress report of geological disposal of TRU waste in Japan, policy and general scheme of future study for the waste disposal in Japan was published by ANRE and JAEA. This annual report summarized aim and progress of individual problem, which was assigned into JAEA in the published policy and general scheme. The problems are as follows; characteristics of TRU waste and its geologic disposal, treatment and waste production, quality control and inspection methodology for waste, mechanical analysis of near-field, data acquisition and preparation on radionuclides migration, cementitious material transition, bentonite and rock alteration in alkaline solution, nitrate effect, performance assessment of the disposal system and decomposition of nitrate as an alternative technology. (author)

2007-04-22

403

Radiation treatment of medical devices and packaging materials. Pt. 1

Object: To permit a valve operation test to be periodically made during plant operation without causing variations in plant power by detecting flow control valve defect on the basis of a valve aperture alteration instruction. Structure: Step signals which are equal in absolute value and opposite in sign are coupled to the input side of flow controllers provided on the recycling loops of two or more recycling flow control systems. With these inputs the aperture of the flow control valve on one side is increased (or reduced) while the aperture of the valve on the other side is reduced (or increased). As a result, the recycling flow rate in the loop on one side is increased (or reduced) while that on the other side is reduced (or increased). Whether the valve is normally operating or not is confirmed by checking the recycling flow rate and valve aperture. (Nakamura, S.).

404

Proteomic analysis of apoptosis induction in human lung cancer cells by recombinant MVL

The first part of the study contains a literature compilation of more than 50 original publications reporting the radiation induced effects in 17 different high polymer materials and glass which are relevant in the manufacturing of medical devices or packing materials. The results collected demonstrate that high energy radiation, i.e. gamma- or X-rays, causes various physical and chemical alterations in high polymer materials. A detailed summary and discussion of the results of the original publications is not included in the present report, it will be presented in the second part of the study. Furthermore, the second part of the study will refer to the aspect of wholesomeness of irradiated medical devices or packing materials in a more general manner of representation. (orig.).

405

British Library Electronic Table of Contents (United Kingdom)

Lung cancer is still difficult to treat by current chemotherapeutic procedures. We recently found that MVL, an anti-HIV lectin from blue-green algae Microcystis viridis, also has antitumor activity. The objective of this study was to investigate apoptosis-inducing activity of recombinant MVL (R-MVL) and proteomic changes in A549 cells, and to identify the molecular pathways responsible for the anti-cancer action of R-MVL. We found that R-MVL induces A549 cells apoptosis in a dose-dependent manner by using MTT assay, fluorescent microscope (FM) and flow cytometry (FCM), and the IC50 was calculated to be 24.12??g/ml. Subsequently, 7 altered proteins in R-MVL-treated A549 cells were identified, including upregulated aldehyde dehydrogenase 1 and ?-actin, and five downregulated proteins: heat s...

2011-01-01

406

Probing anomalous quartic couplings in e{gamma} and {gamma}{gamma} colliders

Probing anomalous quartic couplings in e#gamma# and #gamma##gamma# colliders

We analyze the potential of the e{sup +}e{sup -} linear colliders, operating in the e{gamma} and {gamma}{gamma} modes, to probe anomalous quartic vector-boson interactions through the multiple production of W's and Z's. We examine all SU(2){sub L}(circle times)U(1){sub Y} chiral operators of order p{sup 4} that lead to new four-gauge-boson interactions but do not alter trilinear vertices. We show that the e{gamma} and {gamma}{gamma} modes are able not only to establish the existence of a strongly interacting symmetry breaking sector but also to probe for anomalous quartic couplings of the order of 10{sup -2} at 90% C.L. Moreover, the information gathered in the e{gamma} mode can be used to reduce the ambiguities of the e{sup +}e{sup -} mode.

2001-10-01

407

Pretreatment of empty palm fruit bunch for production of chemicals via catalytic pyrolysis

We analyze the potential of the e"+e"- linear colliders, operating in the e#gamma# and #gamma##gamma# modes, to probe anomalous quartic vector-boson interactions through the multiple production of W's and Z's. We examine all SU(2)_L(circle times)U(1)_Y chiral operators of order p"4 that lead to new four-gauge-boson interactions but do not alter trilinear vertices. We show that the e#gamma# and #gamma##gamma# modes are able not only to establish the existence of a strongly interacting symmetry breaking sector but also to probe for anomalous quartic couplings of the order of 10"-"2 at 90% C.L. Moreover, the information gathered in the e#gamma# mode can be used to reduce the ambiguities of the e"+e"- mode.

2001-10-01

408

British Library Electronic Table of Contents (United Kingdom)

The effect of chemical pretreatments using NaOH, H2O2, and Ca(OH)2 on Empty Palm Fruit Bunches (EPFB) to degrade EPFB lignin before pyrolyis was investigated. Spectrophotometer analysis proved consecutive addition of NaOH and H2O2 decomposed almost 100% of EPFB lignin compared to 44% for the Ca(OH)2, H2O2 system while NaOH and Ca(OH)2 used exclusively could not alter lignin much. Next, the pretreated EPFB was catalytically pyrolyzed. Experimental results indicated the phenolic yields over Al-MCM-41 and HZSM-5 catalysts were 90wt% and 80wt%, respectively compared to 67wt% yield for the untreated sample under the same set of conditions. Meanwhile, the experiments with HY zeolite yielded 70wt% phenols.

2009-01-01

409

Power correlation for vertical axis wind turbines with varying geometries

British Library Electronic Table of Contents (United Kingdom)

Abstract In this paper, a new predictive model that can forecast the performance of a vertical axis wind turbine (VAWT) is presented. The new model includes four primary variables (rotor velocity, wind velocity, air density, and turbine power output) as well as five geometrical variables (rotor radius, turbine height, turbine width, stator spacing, and stator angle). These variables are reduced to include the power coefficient (Cp) and tip speed ratio (TSR). A power coefficient correlation for a novel VAWT (called a Zephyr Vertical axis Wind Turbine (ZVWT)) is developed. The turbine is an adaptation of the Savonius design. The new correlation can predict the turbine's performance for altered stator geometry and varying operating conditions. Numerical simulations with a rotating reference f...

2011-01-01

410

Port-induced erosion prediction and valuation of a local recreational beach

British Library Electronic Table of Contents (United Kingdom)

This study attempts to integrate environmental economics and coastal engineering in managing port-induced coastal erosion occurring at a common beach by using Map Ta Phut port in Thailand as a case study. The existence of the port creates coastal erosion which can be considered an externality that affects local inhabitants, and a port owner and shipping companies can be seen as ?polluters??. Overlaying of aerial photographs provided strong evidence that the coastline was severely eroded after the construction of the port. Coastal engineering softwares, LITPACK and MIKE 21 PMS, were utilized to predict future shoreline positions and investigate wave patterns around the port. The port alters wave climate and the port-induced erosion is jeopardizing a local recreational beach called Nam Rin, ...

2008-01-01

411

Pineal and photoperiodic influences on fat deposition, pelage, and testicular activity in male meadow voles.

Physiologically Based Pharmacokinetic Modeling of Fetal and Neonatal Manganese Exposure in Humans: Describing Manganese Homeostasis during Development

Pinealectomy completely prevented gonadal regression as well as reduction in body weight and white adipose tissue content of the gonadal and retroperitoneal fat deposits in male meadow voles transferred from long to short day lengths. Pineal influences on pelage characteristics depended on which parameter was assessed. For instance, the increase in guard hair length observed in short-day control voles was blocked by pinealectomy; however, a similar increase in underhair length was unaffected by removal of the pineal gland. Photoperiod-dependent changes in fat deposition, testicular activity, and guard hair length presumably rely on altered pineal secretory activity to transduce the effects of day length on the neuroendocrine axis; however, mechanisms independent of pineal activity may be capable of mediating photoperiodic control of underhair growth. PMID:2979644

1988-01-01

412

British Library Electronic Table of Contents (United Kingdom)

Concerns for potential vulnerability to manganese (Mn) neurotoxicity during fetal and neonatal development have been raised due to increased needs for Mn for normal growth, different sources of exposure to Mn, and pharmacokinetic differences between the young and adults. A physiologically based pharmacokinetic (PBPK) model for Mn during human gestation and lactation was developed to predict Mn in fetal and neonatal brain using a parallelogram approach based upon extrapolation across life stages in rats and cross-species extrapolation to humans. Based on the rodent modeling, key physiological processes controlling Mn kinetics during gestation and lactation were incorporated, including alterations in Mn uptake, excretion, tissue-specific distributions, and placental and lactational transfer ...

2011-01-01

413

Physical therapy for chronic pain conditions—A novel approach using mind–body connection

British Library Electronic Table of Contents (United Kingdom)

The treatment of chronic pain conditions continue to present a challenge to the medical field. Physical therapy treatment needs to approach the management of chronic pain differently than the approach used to resolve acute pain. Patients with chronic pain often have many factors that have perpetuated the pain condition. These factors, as well as pain itself, influence their ability to reactivate and perform functional daily activities. Physical therapists can begin to address some of the factors, including fear and deconditioning, by building confidence and body awareness in a self-care model including a slow graduated reactivation program. There is evidence that patients with chronic pain conditions have altered motor control that may be influencing reoccurrences and possible flares of pa...

2011-01-01

414

Photoperiod and gonadal hormones influence odor preferences of the male meadow vole, Microtus pennsylvanicus.

Pentobarbital anesthesia alters neural responses in the precedence effect

Male meadow voles housed in a long photoperiod (14 h light/day, LP) preferred female to male odors, whereas males maintained in a short photoperiod (10 h light/day, SP) did not display preferences for odors of either sex. These odor-preference patterns matched those of free-living males during spring and autumn, respectively. The preference of LP male voles for female over male odors was eliminated by gonadectomy and reinstated by treatment with testosterone. In SP males, although gonadectomy did not affect odor choices, a preference for female odors was induced by testosterone treatment. Treatment with estradiol did not alter odor preferences of LP or SP males. In conjunction with previous result, the present findings suggest that hormonal responsiveness of neural substrates that control odor preferences are sexually dimorphic and may reflect sex differences in reproductive strategies. PMID:1615048

1992-05-01

415

British Library Electronic Table of Contents (United Kingdom)

The precedence effect (PE) is thought to be beneficial for proper localization and perception of sounds. The majority of recent physiological studies focus on the neural discharges correlated with PE in the inferior colliculus (IC). Pentobarbital anesthesia is widely used in physiological studies. However, little is known of the effect of pentobarbital on the discharge of neurons in PE. Neuronal responses in the IC from 23 male SD rats were recorded by standard extracellular recording techniques following presentation of 4ms white noise bursts, presented from either or both of two loud speakers, at different interstimulus delays (ISDs). The neural responses were recorded for off-line analysis before or after intraperitoneal administration of pentobarbital at a loading or maintenance dose. ...

2011-01-01

416

Painful Love--Hispareunia- after Sling Erosion of the Female Partner

British Library Electronic Table of Contents (United Kingdom)

Abstract Introduction.- Sling erosion/extrusion is a complication after suburethral sling insertion for female stress urinary incontinence that occurs in approximately 6% of patients. Symptoms may include vaginal discharge, infections, postcoital bleeding, and alterations of the sexual function. Little is known about the effect of sling erosion on the sexual function of the male partner. Aim.- The aim of this study was to determine male sexual function in partners of women who had undergone sling insertion for stress urinary incontinence and who developed sling erosion postoperatively. Main Outcome Measures.- Main outcome measures were the Brief Male Sexual Function Inventory (BMSFI) and visual analog scale (VAS) scores. Methods.- Male partners of patients who presented with sling erosion ...

2011-01-01

417

Ozone risk for crops and pastures in present and future climates

British Library Electronic Table of Contents (United Kingdom)

Ozone is the most important regional-scale air pollutant causing risks for vegetation and human health in many parts of the world. Ozone impacts on yield and quality of crops and pastures depend on precursor emissions, atmospheric transport and leaf uptake and on the plant?s biochemical defence capacity, all of which are influenced by changing climatic conditions, increasing atmospheric CO2 and altered emission patterns. In this article, recent findings about ozone effects under current conditions and trends in regional ozone levels and in climatic factors affecting the plant?s sensitivity to ozone are reviewed in order to assess implications of these developments for future regional ozone risks. Based on pessimistic IPCC emission scenarios for many cropland regions elevated mean ozone lev...

2009-01-01

418

Ozone health effects

Onset of epilepsy and menarche-Is there any relationship?

Ozone is a principal component of photochemical air pollution endogenous to numerous metropolitan areas. It is primarily formed by the oxidation of NOx in the presence of sunlight and reactive organic compounds. Ozone is a highly active oxidizing agent capable of causing injury to the lung. Lung injury may take the form of irritant effects on the respiratory tract that impair pulmonary function and result in subjective symptoms of respiratory discomfort. These symptoms include, but are not limited to, cough and shortness of breath, and they can limit exercise performance. The effects of ozone observed in humans have been primarily limited to alterations in respiratory function, and a range of respiratory physiological parameters have been measured as a function of ozone exposure in adults and children. These affects have been observed under widely varying (clinical experimental and environmental settings) conditions

1994-07-01

419

British Library Electronic Table of Contents (United Kingdom)

SummaryPurposeWomen with epilepsy have increased frequency of reproductive health problems compared to women without epilepsy. In puberty, reproductive hormonal changes during sexual maturation may affect epilepsy and induce the debut of seizures as indicated in some studies. On the other hand, epileptic activity affects sex hormone function, which may induce alterations in pubertal endocrine maturation and thereby menarche age. We wanted to investigate the relation between epilepsy and menarche age in a larger population of female epilepsy patients.MethodsA retrospective, questionnaire study of a cohort of 265 female outpatients from three Norwegian hospitals and 142 controls, aged 18-45 years was conducted. Parameters regarding epilepsy and reproductive health issues were registered. Per...

2006-01-01

420

Nuclear astrophysics with radioactive ion beams

No association between affective and behavioral dysregulation and parameters of thyroid function in youths

Nuclear astrophysics seeks for a possible explanation of the observed abundance distribution of various elements and their isotopes in the universe. Most of the relevant nuclear reactions take place in thermally equilibrium environments with bare nuclei, rather than accelerated and head-on colliding situations with low ionisation states of reactant atoms and molecules that are emulated in the laboratories. Moreover, the temperature of the astrophysical environments is quite often low compared to the centre-of-mass energy of the projectile nuclides, that is required for the reaction to be meaningfully investigated in the laboratory. Therefore, an extrapolation of the data on the reaction cross sections to very low energies and to extremely high density situations is generally called for, which are substantially altered every now and then for a number of astrophysically important reactions. The radioactive ion beams will provide us important data on some of those ...

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421

British Library Electronic Table of Contents (United Kingdom)

Objective: Evidence from adults suggests that changes in thyroid function are associated with the development of bipolar disorder (BD) and severe mood dysregulation. A dysregulation profile based on the Child Behavior Checklist (CBCL-DP) describes a phenotype with severe mood problems in youth. The present study investigated whether altered thyroid functioning in youths is associated with the severe mood dysregulation symptoms characterized by the CBCL-DP. Methods: We analyzed the thyroid function data from 262 children and adolescents (n=262 for serum TSH, n=148 for free triiodothyronine [fT3] and n=153 for free thyroxine [fT4]) with their CBCL-DP composite score. We created and compared high CBCL-DP and low CBCL-DP subgroups with regard to their serum TSH, fT3 and fT4 concentrations as w...

2011-01-01

422

Nickel induced structural and functional alterations in mouse Leydig cells in vitro

British Library Electronic Table of Contents (United Kingdom)

The present study was aimed at investigating effects of nickel (NiCl2) on secretion of testosterone (T), cell viability, ultrastructure and apoptosis in mouse Leydig cells. Testosterone release was measured after 48h of culture with 15.67, 31.25, 62.5, 125, 250, 500 and 1000mmol/L NiCl2 or without NiCl2 using radioimmunoassay. Cell viability was assessed by a MTT (metabolic activity assay). Quantification of apoptotic cells was performed using TUNEL assay and the ultrastructural changes were analyzed using transmission electron microscopy. The viability was decreased after addition of >=250mmol/L NiCl2. A concentration-dependent depression of T production was observed. The percentage of apoptotic cells was significantly increased only after addition of 125, 250 and 1000mmol/L NiCl2. After ...

2011-01-01

423

New Ideas in the Theory of Extrasolar Giant Planets and Brown Dwarfs

Negotiating Conflict: Rifā'a Rāfi' al-TahTāwi and the Translation of the "Other" in Nineteenth-century Egypt

We summarize and extend recent work on the theory of extrasolar giant planets (EGPs) and brown dwarfs, paying particular attention to Gliese 229 B, the albedos of EGPs, the compositions of substellar atmospheres, the connections with the giant planets in the solar system, cloud physics, and non-gray spectral synthesis. The role of condensates in altering the optical spectrum of Gliese 229 B is explored, as are the systematics of the reflection spectra from extrasolar giant planets near their primaries. In addition, we discuss the role of convection and disequilibrium chemistry in explaining the anomalous detection of CO in Gliese 229 B. Throughout, we highlight the distinctive chemistry that defines this new class of objects and set goals for future study.

1998-01-01

424

British Library Electronic Table of Contents (United Kingdom)

This paper analyses the role of the translator in the representation of alterity and the construction of national identity, with reference to the work of a nineteenth-century Egyptian translator, essayist and educationalist, Rif?'a R?fi' al-TahT?wi (1801-1874). The essay takhliS al-ibriz fi talkhiS b?ris ("The Extraction of Gold in the Summarizing of Paris") includes numerous examples of constructive translation and representation, which familiarised and legitimised the "other" through the identification of parallels, common values and experience. Al-TahT?wi negotiated between conflicting discourses of modernism and traditionalism, and it is argued that the issues of representation raised in his work are of particular relevance to contemporary concerns in the ...

2007-01-01

425

Multidate image analysis of forest degradation in equatorial Africa

Mottling score predicts survival in septic shock

A section of the northern margin of the Guineo-Congolian rain forest of the Central African Republic is studied to determine to what extent deforestation is evolving. Three sites are presented to highlight the diversity in local environmental settings at the northern margin of the closed equatorial rain forest: the contiguous equatorial rain forest, the boundary between the closed rain forest and the grasslands, and a predominantly secondary grassland environment. Proven image processing procedures for determining land cover and vegetation vigor were applied to Landsat MSS data to determine land cover characteristics and identify alterations in land cover that indicate potentially degraded forest environments. Land cover was independently assessed using spectral signatures determined from a statistical clustering routine. The images presented and image analyses contribute insights and information to an ongoing effort to determine more reliable data on the status of ...

1990-12-01

426

British Library Electronic Table of Contents (United Kingdom)

Background Experimental and clinical studies have identified a crucial role of microcirculation impairment in severe infections. We hypothesized that mottling, a sign of microcirculation alterations, was correlated to survival during septic shock. Methods We conducted a prospective observational study in a tertiary teaching hospital. All consecutive patients with septic shock were included during a 7-month period. After initial resuscitation, we recorded hemodynamic parameters and analyzed their predictive value on mortality. The mottling score (from 0 to 5), based on mottling area extension from the knees to the periphery, was very reproducible, with an excellent agreement between independent observers [kappa?=?0.87, 95% CI (0.72?0.97)]. Results Sixty patients were included. The SOFA scor...

2011-01-01

427

Modification of streaming potential by precipitation of calcite in a sand-water system: laboratory measurements in the pH range from 4 to 12

British Library Electronic Table of Contents (United Kingdom)

SUMMARY Spontaneous potentials associated with volcanic activity are often interpreted by means of the electrokinetic potential, which is usually positive in the flow direction (i.e. Zeta potential of the rock is negative). The water-rock interactions in hydrothermal zones alter the primary minerals leading to the formation of secondary minerals. This work addresses the study of calcite precipitation in a sand composed of 98 per cent quartz and 2 per cent calcite using streaming potential measurements. The precipitation of calcite as a secondary mineral phase, inferred by high calcite saturation indices and by a fall in permeability, has a significant effect on the electrokinetic behaviour, leading to a significant reduction in the Zeta potential (in absolute value) and even a change in si...

2006-01-01

428

Modification of intergrain connectivity, upper critical field anisotropy and critical current density in ion irradiated MgB{sub 2} films

Metabolic engineering of carotenoid accumulation by creating a metabolic sink

We compare the effect of isotropic point defects vis a vis extended defects on the inter and intra grain properties of superconducting MgB{sub 2} thin films. In a recent paper Gandikota et al. [Appl. Phys. Lett. 86 (2005) 012508] reported that after 200 MeV {alpha} particle irradiation intergrain connectivity remains unaffected. Our results on the contrary indicate that connectivity does depend on irradiation dose and type of ions used. We ascertain that extended defects alter the {sigma} band properties of this two-band superconductor more effectively than the point defects. The improvement in upper critical field and critical current density is intricately related to the type and density of defects created.

2006-08-01

429

British Library Electronic Table of Contents (United Kingdom)

Carotenoids are highly beneficial for human nutrition and health because they provide essential nutrients and important antioxidants in our diets. However, many food crops, especially the major staple crops contain only trace to low amounts of carotenoids. Although significant progress has been made in developing food crops rich in carotenoids by altering the expression of carotenoid biosynthetic genes, in many cases it has proved to be difficult to reach the desired levels of carotenoid enrichment. The recent identification and characterization of a novel gene mutation in cauliflower reveals that creating a metabolic sink to sequester carotenoids is an important mechanism to control carotenoid accumulation in plants. The successful demonstration of increased carotenoid accumulation in ass...

2007-01-01

430

Mammary gland tumor formation in transgenic mice overexpressing stromelysin-1

Landscape approach for quantifying land use land cover change (1972?2006) and habitat diversity in a mining area in Central India (Bokaro, Jharkhand)

An intact basement membrane (BM) is essential for the proper function, differentiation and morphology of many epithelial cells. The disruption or loss of this BM occurs during normal development as well as in the disease state. To examine the importance of BM during mammary gland development in vivo, we generated transgenic mice that inappropriately express autoactivating isoforms of the matrix metalloproteinase stromelysin-1. The mammary glands from these mice are both functionally and morphologically altered throughout development. We have now documented a dramatic incidence of breast tumors in several independent lines of these mice. These data suggest that overexpression of stromelysin-1 and disruption of the BM may be a key step in the multi-step process of breast cancer.

1995-06-01

431

British Library Electronic Table of Contents (United Kingdom)

The rate and intensity of land use land cover (LULC) change has increased considerably during the past couple of decades. Mining brings significant alterations in LULC specifically due to its impact on forests. Parts of Central India are well endowed with both forests and minerals. Here, the conflict between human interests and nature has intensified over time. Monitoring and assessment of such conflicts are important for land management and policy making. Remote sensing and Geographical Information System have the potential to serve as accurate tools for environmental monitoring. Understanding the importance of landscape metrics in land use planning is challenging but important. These metrics calculated at landscape, class, and patch level provide an insight into changing spatiotemporal d...

2010-01-01

432

Investigation of passive films on nickel Alloy 690 in lead-containing environments

Investigating the biological and clinical significance of human dysbioses

Passive films formed on Alloy UNS N06690 were investigated in simulated crevice chemistries. It was found the role of lead in corrosion processes is strongly dependent on the pH value of the testing solutions. At pH 1.5 the effect of lead is narrowly noticeable; while at pH 12.7, lead has a significant influence on the electrochemical performance of alloy UNS N06690. The lead alters the surface morphologies at both pH and account for higher hydroxide content in the surface film at pH 12.7. The lead incorporation hinders the formation of spinel oxides during the passivation in alkaline solution. Nanoindentation tests indicate a significant lead-induced degradation in the mechanical properties of passive films. The passivation degradation is attributed to detrimental effects of lead via interrupting the dehydration process and hindering the formation of protective layers on the alloy surface.

2008-09-01

433

British Library Electronic Table of Contents (United Kingdom)

Culture-independent microbiological technologies that interrogate complex microbial populations without prior axenic culture, coupled with high-throughput DNA sequencing, have revolutionized the scale, speed and economics of microbial ecological studies. Their application to the medical realm has led to a highly productive merger of clinical, experimental and environmental microbiology. The functional roles played by members of the human microbiota are being actively explored through experimental manipulation of animal model systems and studies of human populations. In concert, these studies have appreciably expanded our understanding of the composition and dynamics of human-associated microbial communities (microbiota). Of note, several human diseases have been linked to alterations in th...

2011-01-01

434

Inhibition of prepenetration processes of the powdery mildew Golovinomyces orontii on host inflorescence stems is reduced in the Arabidopsis cuticular mutant cer3 but not in cer1

British Library Electronic Table of Contents (United Kingdom)

The obligate biotrophic fungus Golovinomyces orontii causes powdery mildew (PM) disease on its host Arabidopsis thaliana. Most research on the G. orontii?Arabidopsis pathosystem uses rosette leaves as experimental materials, so little is known about the behavior of G. orontii on other Arabidopsis organs. We thus conducted microscopic analyses of the PM infection process on leaves, stems, fruits and roots of Arabidopsis. Adaxial and abaxial surfaces of mature rosette leaves supported G. orontii infection, but growth was somewhat suppressed on cauline leaves. Prepenetration processes (germination and appressorium formation) were strongly inhibited on stems, fruits and roots. To test the effect of alterations in the Arabidopsis cuticle on the inhibition of prepenetration processes on stems, w...

2011-01-01

435

Impact of ocean pollution

Identification of mutations leading to the Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA

Man's wastes are polluting the oceans from various sources and delivered by various routes. The result has been the loss of habitat and the irreversible altering of marine ecosystems. Development in the coastal zones and offshore activities that disrupt biologically sensitive areas have led to international negotiations to regulate these impacts and develop a law of the sea. Basic to international cooperation, however, is the need to develop answers to questions about the ecological consequences of development programs and the carrying capacity of the oceans. Current information does not demonstrate long-term global impacts, making it difficult to detect and predict incremental changes and causal relationships. Society needs to determine how much environmental damage it will accept and then to develop appropriate technology, such as biomonitoring. Society also needs to reexamine its positions on technology-based regulatory controls to see if the effects of ...

1981-03-01

436

Hypomethylation and induction of retinoic acid receptor beta 2 by concurrent action of adenosine analogues and natural compounds in breast cancer cells

The Lesch-Nyhan (LN) syndrome is a severe X chromosome-linked disease that results from a deficiency of the purine salvage enzyme hypoxanthine phosphoribosyltransferase (HPRT). The mutations leading to the disease are heterogeneous and frequently arise as de novo events. The authors have identified nucleotide alterations in 15 independently arising HPRT-deficiency cases by direct DNA sequencing of in vitro amplified HPRT cDNA. They also demonstrate that the direct DNA sequence analysis can be automated, further simplifying the detection of new mutations at this locus. The mutations include DNA base substitutions, small DNA deletions, a single DNA base insertion, and errors in RNA splicing. The application of these procedures allows DNA diagnosis and carrier identification by the direct detection of the mutant alleles within individual families affected by LN.

1989-03-01

437

British Library Electronic Table of Contents (United Kingdom)

DNA methylation is considered as a potential cause of aberrations in regulation of gene expression during carcinogenesis. Therefore, changes in DNA methylation patterns may be targets for chemoprevention. In the present study, we investigated effects of all-trans retinoic acid (ATRA), vitamin D3, and resveratrol alone and in combination with adenosine analogues: 2-chloro-2'-deoxyadenosine (2CdA) and 9-beta-d-arabinosyl-2-fluoroadenine (F-ara-A), on methylation and expression of retinoic acid receptor beta 2 (RARbeta2) in MCF-7 and MDA-MB-231 breast cancer cell lines. Alterations in methylation and expression levels after treatment of cells with the tested compounds were evaluated by methylation-sensitive restriction analysis (MSRA) and real-time PCR, respectively. RARbeta2 promoter in the ...

2010-01-01

438

Hydrological Classification of Natural Flow Regimes to Support Environmental Flow Assessments in Intensively Regulated Mediterranean Rivers, Segura River Basin (Spain)

British Library Electronic Table of Contents (United Kingdom)

Hydrological classification constitutes the first step of a new holistic framework for developing regional environmental flow criteria: the ?Ecological Limits of Hydrologic Alteration (ELOHA)?. The aim of this study was to develop a classification for 390 stream sections of the Segura River Basin based on 73 hydrological indices that characterize their natural flow regimes. The hydrological indices were calculated with 25?years of natural monthly flows (1980/81?2005/06) derived from a rainfall-runoff model developed by the Spanish Ministry of Environment and Public Works. These indices included, at a monthly or annual basis, measures of duration of droughts and central tendency and dispersion of flow magnitude (average, low and high flow conditions). Principal Component Analysis (PCA) indi...

2011-01-01

439

Human brain development in infants with PET and FDG

High-gain harmonic generation free electron laser seeded by few-cycle laser

The authors used studies of local cerebral metabolic rate for glucose (LCMRGlc) to examine development of cerebral organization in 5 days to 1 year old children. A group (n=8) of infants with diverse pediatric disorders allowed investigation of developmental changes in LCMRGlc, while also providing relevant clinical management information. Patients consisted of questionable and definite neonatal seizures, cerebral embolism from cardiac sources, and otherwise normal infants with facial nevi with consideration of Sturge-Weber. Gradual increase in cortical LCMRGlc coincides with suppression of intrinsic subcortical reflexes present in all newborns. Two retarded children (2 years old) showed LCMRGlc developmental patterns of a few days old, which corresponded to their functional and mental status. These studies illustrate great potential of PET to study normal and altered states of human brain development.

1985-06-02

440

Geothermal studies of the Outokumpu Deep Drill Hole, Finland: Vertical variation in heat flow and palaeoclimatic implications

Tunable output wavelength is an important character of a free electron laser (FEL). As generally understood, to alter the wavelength of a high-gain harmonic generation (HGHG) FEL laser, the seed laser must be tunable. A few-cycle, high intensity laser obtained by optical compression usually has pretty broad bandwidth in the spectral domain, which can be used as the seed laser of a HGHG and may open the possibility to approach a HGHG scheme with fully tunable wavelength. In this paper, a HGHG FEL seeded by a few-cycle laser is theoretically discussed and numerically investigated. A few-cycle seed laser significantly influences HGHG FEL performance, especially in output wavelength tuning. For a HGHG FEL seeded by a 4-cycle 786 nm laser, output wavelength tunability range up to 14.9% of the central wavelength 262 nm is observed by adjusting the resonant condition.

2008-06-07

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441

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Detailed geothermal studies of deep drill holes provide insights to heat transfer processes in the crust, and allow separation of different factors involved, such as palaeoclimatic and structural conductive effects as well as advective fluid flow effects. We present high resolution geothermal results of the 2516m deep Outokumpu Deep Drill Hole in eastern Finland drilled in 2004-2005 into a Palaeoproterozoic formation with metasedimentary rocks, ophiolite-derived altered ultramafic rocks and pegmatitic granite. The down-hole temperatures have been logged five times after end of drilling and extend to day 948 after drilling. The hole is completely cored (79% core coverage) and thermal conductivity measurements were done at 1m intervals. The geothermal results on temperature gradient, thermal...

2011-01-01

442

Geology, physical properties, and surface effects at Discus Thrower Site, Yucca Flat, Nevada Test Site

Genomic survey of prepulse inhibition in mouse chromosome substitution strains

Geologic studies in connection with Project Discus Thrower have furnished detailed stratigraphic and structural information about northwestern Yucca Flat, Nevada Test Site. The Paleozoic rocks consist of a lower carbonate sequence, argillite of the Eleana Formation, and an upper carbonate sequence. The distribution of these rocks suggests that both top and bottom of the Eleana are structural contacts, probably thrusts or reverse faults. The overlying tuff includes several units recognized in the subsurface, such as the Fraction Tuff and tuff of Redrock Valley. Other units recognized include bedded tuff associated with the Grouse Canyon Member of Belted Range Tuff, and the Rainier Mesa and Ammonia Tanks Members of the Timber Mountain Tuff. The Timber Mountain and Grouse Canyon are extensively altered to montmorillonite (a swelling clay), possibly as a result of ponding of alkaline water. The overlying alluvium locally contains at the base a clayey, tuffaceous ...

443

British Library Electronic Table of Contents (United Kingdom)

Prepulse inhibition (PPI) is a measure of sensorimotor gating, a pre-attentional inhibitory brain mechanism that filters extraneous stimuli. Prepulse inhibition is correlated with measures of cognition and executive functioning, and is considered an endophenotype of schizophrenia and other psychiatric illnesses in which patients show PPI impairments. As a first step toward identifying genes that regulate PPI, we performed a quantitative trait locus (QTL) screen of PPI phenotypes in a panel of mouse chromosome substitution strains (CSSs). We identified five CSSs with altered PPI compared with the host C57BL/6J strain: CSS-4 exhibited decreased PPI, whereas CSS-10, -11, -16 and -Y exhibited higher PPI compared with C57BL/6J. These data indicate that A/J chromosomes 4, 10, 11, 16 and Y harbor...

2009-01-01

444

Genesis of the Jurassic Carbonate-Hosted Pb-Zn Deposits of Jebel Ressas (North-Eastern Tunisia): Evidence from Mineralogy, Petrography and Trace Metal Contents and Isotope (O, C, S, Pb) Geochemistry

British Library Electronic Table of Contents (United Kingdom)

Abstract The Jebel Ressas Pb-Zn deposits in North-Eastern Tunisia occur mainly as open-space fillings (lodes, tectonic breccia cements) in bioclastic limestones of the Upper Jurassic Ressas Formation and along the contact of this formation with Triassic rocks. The galena-sphalerite association and their alteration products (cerussite, hemimorphite, hydrozincite) are set within a calcite gangue. The Triassic rocks exhibit enrichments in trace metals, namely Pb, Co and Cd enrichment in clays and Pb, Zn, Cd, Co and Cr enrichment in carbonates, suggesting that the Triassic rocks have interacted with the ore-bearing fluids associated with the Jebel Ressas Pb-Zn deposits. The 18O content of calcite associated with the Pb-Zn mineralization suggests that it is likely to have precipitated from a fl...

2011-01-01

445

Generation of N-ethyl-N-nitrosourea-induced mouse mutants with deviations in hematological parameters

British Library Electronic Table of Contents (United Kingdom)

Research on hematological disorders relies on suitable animal models. We retrospectively evaluated the use of the hematological parameters hematocrit (HCT), hemoglobin (HGB), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), red blood cell count (RBC), white blood cell count (WBC), and platelet count (PLT) in the phenotype-driven Munich N-ethyl-N-nitrosourea (ENU) mouse mutagenesis project as parameters for the generation of novel animal models for human diseases. The analysis was carried out on more than 16,000 G1 and G3 offspring of chemically mutagenized inbred C3H mice to detect dominant and recessive mutations leading to deviations in the levels of the chosen parameters. Identification of animals exhibiting altered valu...

2011-01-01

446

Food reward functions as affected by obesity and bariatric surgery

British Library Electronic Table of Contents (United Kingdom)

Roux-en-Y gastric bypass surgery (RYGB) remains to be the most effective long-term treatment for obesity and its associated comorbidities, but the specific mechanisms involved remain elusive. Because RYGB patients appear to no longer be preoccupied with thoughts about food and are satisfied with much smaller meals and calorically dilute foods, brain reward mechanisms could be involved. Just as obesity can produce maladaptive alterations in reward functions, reversal of obesity by RYGB could normalize these changes or even further reset the food reward system through changes in gut hormone secretion, aversive conditioning and/or secondary effects of weight loss. Future studies with longitudinal assessments of reward behaviors and their underlying neural circuits before and after surgery wil...

2011-01-01

447

Failure analyses and weld repair of boiler feed water pumps

Engineering Properties of Polymeric-Based Antimicrobial Films for Food Packaging: A Review

During a regular inspection of the Boiler Auxiliaries at one of the Dutch Electricity Production Companies serious cracks were found in the cover and casings of the feed water circulation pumps in two units after 108.000 and 122.000 hours of boiler operation. Kema Laboratories carried out Failure analyses on boat samples at the cracked areas. Corrosion fatigue cracking was found on the inner side of the GS-24CrNiMo325 casing. Shop Weld repairs were carried out using a newly developed mechanized Plasma Welding Technique. The repaired feed water circulation pumps showed no problems alter several years of operation. The costs of repair were substantially lower than the costs of replacement. (orig.) 3 refs.

1998-12-31

448

British Library Electronic Table of Contents (United Kingdom)

The concept of antimicrobial packaging has received great attention because of its potential to enhance food safety. Several studies have explored its applications and effectiveness to suppress pathogenic microorganisms. However, few studies have analyzed the alterations caused in the engineering properties of food-packaging polymers after the incorporation of antimicrobials. Such information is very important to understand the feasibility of producing antimicrobial packaging films on the industrial scale. This review explores the work done so far to evaluate how the incorporation of antimicrobial substances affects the properties of food-packaging systems. This article also emphasizes diffusion studies on antimicrobial substances through packaging films and the analytical solutions used t...

2011-01-01

449

Enforcing Semantic Integrity on Untrusted Clients in Networked Virtual Environments

Effects of thermal constancy and seasonal temperature displacement on community structure of stream macroinvertebrates

During the last years, large-scale simulations of realistic physical environments which support the interaction of multiple participants over the Internet have become increasingly available and economically significant, most notably in the computer gaming industry. Such systems, commonly called networked virtual environments (NVEs), are usually based on a client-server architecture where for performance reasons and bandwidth restrictions, the simulation is partially deferred to the clients. This inevitable architectural choice renders the simulation vulnerable to attacks against the semantic integrity of the simulation: malicious clients may attempt to compromise the physical and logical laws governing the simulation, or to alter the causality of events a posteriori. In this paper, we initiate the systematic study of semantic integrity in NVEs from a security point of view. We argue that naive policies to enforce semantic integrity involve intolerable network load, ...

2005-01-01

450

Effects of grazer identity on the probability of escapes by a canopy-forming macroalga

Diurnal and seasonal thermal constancy, a greatly delayed seasonal temperature maximum, and summer cool and winter warm conditions characterize the stream environment below a deep-release dam in Colorado. Low diversity index and equitability values and changes in macroinvertebrate species composition may result from failure of the temperature regime to provide the thermal stimuli essential for various life-cycle phenomena. It is hypothesized that the following sublethal effects, directly or indirectly resulting from the modified temperature regime, may further alter macroinvertebrate community structure: reduction of niche overlap and a shift toward an equilibrium community as a consequence of reduced environmental fluctuation; more intense competition associated with greater productivity; elimination of major invertebrate predators; and failure of the limited temperature range to provide optimal temperatures for various physiological processes. Effects of the ...

451

British Library Electronic Table of Contents (United Kingdom)

Through their grazing activities limpets have an important role in controlling macroalgal abundance and as a result the structure and dynamics of rocky shore assemblages. Using two congeneric limpet species, with different biogeographic distributions, and whose ranges are expected to alter with climatic warming, we separated the magnitude of their grazing activity over time and the subsequent consequence for macroalgal growth.The northern/boreal limpet, Patella vulgata (L.), consistently grazed more than the southern/lusitanian limpet, P. depressa (Pennant), particularly during spring and summer when P. depressa was reproductively active. Individuals of Fucus vesiculosus (L.) that settled during this time were able to grow to a size where they escaped the grazing activities of P. depressa,...

2007-01-01

452

Effect of biodegradation on vanadylporphyrin distribution

Differential facilitative and competitive effects of a dominant macrophyte in grazed subtropical wetlands

Oils, tars, and degraded oils of varying degrees of biodegradation occur in the Permian Phosphoria Formation along the eastern flank of the Wind River mountains, Wyoming, US. Biodegradation has altered the isomer distributions of steranes, the hopanes, and the mono- and triaromatised steroids in these oils. The conventional maturity parameters based on these biomarkers are of limited use in estimating their maturity. On the other hand, even in severely biodegraded oils the vanadylporphyrin distributions are unaltered. Because of this, the Porphyrin Maturity Parameter (PMP) derived from the vanadylporphyrin distribution is an ideal parameter for estimating the maturity of these oils. This study also confirms the previous observation that T[sub s] and T[sub m] are unaffected by even severe biodegradation. 14 refs., 5 figs., 2 tabs.

1993-05-01

453

British Library Electronic Table of Contents (United Kingdom)

Summary 1.-Plant-plant interactions fluctuate between competition and facilitation depending upon ecological conditions and species traits. Facilitative interactions are expected to increase in frequency via associational defences with increasing consumer pressure. The ability of species to cope with competition and/or ecological stressors may alter the outcome of plant-plant interactions. 2.-We conducted a transplant experiment to determine if native and non-native grasses and forbs respond similarly to interactions with Juncus effusus L., an unpalatable benefactor species, along a grazing intensity gradient in two contrasting pasture types: intensively managed and semi-natural. We expected competitive taller, erect species (grasses) and non-natives to obtain stronger facilitative effects...

2011-01-01

454

Dietary turmeric modulates DMBA-induced p21ras, MAP kinases and AP-1/NF-?B pathway to alter cellular responses during hamster buccal pouch carcinogenesis

British Library Electronic Table of Contents (United Kingdom)

The chemopreventive efficacy of turmeric has been established in experimental systems. However, its mechanism(s) of action are not fully elucidated in vivo. The present study investigates the mechanism of turmeric-mediated chemoprevention in 7,12-dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis at 2, 4, 6, 10 and 12?weeks. Dietary turmeric (1%) led to decrease in DMBA-induced tumor burden and multiplicity, and enhanced the latency period in parallel, to its modulatory effects on oncogene products and various cellular responses during HBP tumorigenesis. DMBA-induced expression of ras oncogene product, p21 and downstream target, the mitogen-activated protein kinases were significantly decreased by turmeric during HBP carcinogenesis. Turmeric also diminished ...

2008-01-01

455

Diagenesis and porosity development in the carbonate reservoirs in the Ordovician Majiagou Formation, northern Ordos Basin, China

Development of a petrographic technique for the quality control of concrete mixes used in mines. Final report; Mise au point d`une technique petrographique pour le controle de qualite des betons miniers. Rapport final

The carbonate rocks of the Lower Ordovician Majiagou Formation represent important hydrocarbon reservoirs in the Ordos basin in the western part of the North China plate. Cores and thin sections from more than 60 wells were examined. The main reservoir in the Majiagou Formation consists of fine crystalline dolomite, which was altered by meteoric water during an extended subaerial exposure. Three phreatic zones have been identified in the Majiagou Formation in the northern part of the basin. Oxygen isotopes and fluid inclusion microthermometry data indicates that the porosity developed during the early karst event was considerably reduced by the late-stage, coarse crystalline calcite cements that appear to be related to hydrothermal fluids during deep burial. It was concluded that the main factors that determine the porosity and permeability of these carbonate reservoirs are dolomitization, karstification, and calcite cementation.

2006-07-01

456

Development and application of a diaphragm micro-pump with piezoelectric device

Petrographical analysis is a microscopical analytical tool which allows definition of the exact nature of the components of different concrete mixes, as well as definition of internal structures and alteration processes. In order to improve the precision and the swiftness of the petrographical analysis, an automated image analysis system has been developed. In the next stage of this study, which consisted in the testing of new concrete mixes containing fluidised bed ashes, petrography has been used, not only to identify the nature of the mix components, but also assess the behaviour of these new concrete mixes when exposed to aggressive conditions.

1997-11-01

457

British Library Electronic Table of Contents (United Kingdom)

In this study, a new type of thin, compact, and light weighed diaphragm micro-pump has been successfully developed to actuate liquid by the vibration of a diaphragm. The micro-diaphragm pump with two valves is fabricated in an aluminum case by using highly accurate CNC machine, and the cross-section dimension is 28?mm??5?mm. Both valves and diaphragm are manufactured from PDMS. The amplitude of vibration by a piezoelectric device produces an oscillating flow and alters the chamber volume by the curvature change of a diaphragm. Several experimental set-ups for performance tests in a single micro-diaphragm pump, isothermal flow open system, and a closed liquid cooling system are designed and implemented. The performance of a one-side actuating micro-diaphragm pump is affected by the design ...

2008-01-01

458

Detection of behavioral alterations and learning deficits in mice lacking synaptophysin

British Library Electronic Table of Contents (United Kingdom)

The integral membrane protein synaptophysin is one of the most abundant polypeptide components of synaptic vesicles. It is not essential for neurotransmission despite its abundance but is believed to modulate the efficiency of the synaptic vesicle cycle. Detailed behavioral analyses were therefore performed on synaptophysin knockout mice to test whether synaptophysin affects higher brain functions. We find that these animals are more exploratory than their wild type counterparts examining novel objects more closely and intensely in an enriched open field arena. We also detect impairments in learning and memory, most notably reduced object novelty recognition and reduced spatial learning. These deficits are unlikely caused by impaired vision, since all electroretinographic parameters measur...

2009-01-01

459

Detecting retroviral sequences in chronic fatigue syndrome.

Decreased dendritic spine density and abnormal spine morphology in Fyn knockout mice

XMRV or xenotropic murine leukemia virus-related retrovirus, a recently discovered retrovirus, has been linked to both prostate cancer and chronic fatigue syndrome (CFS). Recently, the teams of Drs. Shyh-Ching Lo and Harvey Alter discovered the presence of sequences closely related to XMRV in the blood of 86.5% of patients with CFS [1]. These findings are important because since the initial discovery of XMRV in CFS, several studies have failed to find XMRV in specimens collected from CFS patients. While the current study also did not find XMRV in CFS, Lo et al. did detect sequences that belong to polytropic mouse endogenous retroviruses (PMV), which share considerable similarity with XMRV. Criteria for future studies that will help bring greater clarity to the issue of retroviral sequences in CFS are proposed below. PMID:21994623

2010-11-03

460

British Library Electronic Table of Contents (United Kingdom)

Fyn is a Src-family tyrosine kinase that affects long term potentiation (LTP), synapse formation, and learning and memory. Fyn is also implicated in dendritic spine formation both in vitro and in vivo. However, whether Fyn's regulation of dendritic spine formation is brain-region specific and age-dependent is unknown. In the present study, we systematically examined whether Fyn altered dendritic spine density and morphology in the cortex and hippocampus and if these effects were age-dependent. We found that Fyn knockout mice trended toward a decrease in dendritic spine density in cortical layers II/III, but not in the hippocampus, at 1month of age. Additionally, Fyn knockout mice had significantly decreased dendritic spine density in both the cortex and hippocampus at 3months and 1year, an...

2011-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Computed tomography of middle ear cholesteatomas without tympanic membrane perforation

Color change during the surface preparation stages of metal ceramic alloys

The growth of a middle ear cholesteatoma behind a normal tympanic membrane is a rate though possible event. In such cases, CT may provide useful information for diagnosis. The results are presented of a CT study carried out on 14 patients affected with unilateral conductive hearing loss and with normal tympanic membrane. CT allowed the diagnosis of meddle ear cholesteatoma to be made in all cases. All patients were treated with surgery: 8 of them underwent tympanoplasty and 6 explorative tympanotomy. While the diagnosis of cholesteatoma was confirmed in 13 patients, in 1 case tympanosclerosis was diagnosed. CT diagnosis of middle ear cholesteatoma is based on the demonstration of a low-density soft-tissue mass, in association with bone erosion or ossicular dislocation. The author emphasizes the difficulty of a CT diagnosis of cholesteatoma in the patients with middle ear soft-tissue masses in the absence of bone alterations.

1991-01-01

462

British Library Electronic Table of Contents (United Kingdom)

Statement of problem Even though metal ceramic restorations (MCRs) are widely used by clinicians, the influence of the metal on the color of overlaying porcelain is unknown. Purpose The purpose of this study was to analyze the color alterations of different types of metal ceramic alloys during several stages of metal surface preparation and to determine the effect of those changes on the resulting color of opaque porcelain (OP). Material and Methods Seven different types of alloys (3 base metal, 3 noble, and 1 high noble) were used to prepare disk-shaped specimens (1 mm x 10 mm, n=3), followed by OP application (0.1 mm). Lab values of specimens were recorded after different stages of metal surface preparation (ingot, after casting, after oxidation, and after the OP application) in addition...

2011-01-01

463

Coalification by clay-catalyzed oligomerization of plant monomers. [Methyleugenol

Chromosomal rearrangement segregating with adrenoleukodystrophy: A molecular analysis

During this report period, we have obtained a model of montmorillonite clay, and this model has been of great assistance in visualizing how the chemistry of substrate molecules might be altered as it occurs on the surface of the clay. A stereochemical representation of this montmorillonite model is shown. Of particular significance, this model indicates that hydroxyl groups are located in the center of each siloxane ring on the surface of the montmorillonite clay. These hydroxyl groups might serve to bond substrate molecules to the surface of the clay. The next step in our systematic examination of the radical cation-initiated dimerization of plant monomers from the C{sub 6}-C{sub 3} pool of shikimic acid metabolites was to study the dimerization of cinnamic acid and its derivatives. In the next block of research, we examined the reaction of montmorillonite clay (K-10) with methyleugenol. 2 refs.

1990-01-01

464

Characterization of surface defects after flanging of metallic sheets

The relationship between X chromosome-linked adrenoleukodystrophy and the red/green color pigment gene cluster on Xq28 was investigated in a large kindred. The DNA in a hemizygous male showed altered restriction fragment sizes compatible with at least a deletion extending from the 5[prime] end of the color pigment genes. Segregation analysis using a DNA probe within the color pigment gene cluster showed significant linkage with adrenoleukodystrophy (logarithm of odds score of 3.19 at [theta] = 0.0). These data demonstrate linkage, rather than association, between a unique molecular rearrangement in the color pigment gene cluster and adrenoleukodystrophy. The DNA changes in this region are thus likely to be helpful for determining the location and identity of the responsible gene. 33 refs., 4 figs.

1993-10-15

465

British Library Electronic Table of Contents (United Kingdom)

This paper deals with surface defects of automobile outer panels, which alter significantly the vehicle quality. Such defects occur during springback, after forming or flanging steps, and are characterized by concave depression of small amplitude over the convex shape of the part. The aim of this work is to reproduce at the laboratory scale a surface defect that occurs after flanging on a geometry similar to a door upper corner. A dedicated device has been designed in order to generate small size surface defects during flanging of metallic thin sheets. The outer surface of the sample was digitalized and the spatial geometry of the defect was evaluated from curvature change along 2D profiles. This study shows that the flanging height does not influence significantly the surface defect geome...

2011-01-01

466

Banana peel extract mediated synthesis of gold nanoparticles

British Library Electronic Table of Contents (United Kingdom)

Gold nanoparticles were synthesized by using banana peel extract (BPE) as a simple, non-toxic, eco-friendly 'green material'. The boiled, crushed, acetone precipitated, air-dried peel powder was used to reduce chloroauric acid. A variety of nanoparticles were formed when the reaction conditions were altered with respect to pH, BPE content, chloroauric acid concentration and temperature of incubation. The reaction mixtures displayed vivid colors and UV-vis spectra characteristic of gold nanoparticles. Dynamic light scattering (DLS) studies revealed that the average size of the nanoparticles under standard synthetic conditions was around 300nm. Scanning electron microscopy and energy dispersive spectrometry (EDS) confirmed these results. A coffee ring phenomenon, led to the aggregation of th...

2010-01-01

467

Banana peel extract mediated novel route for the synthesis of silver nanoparticles

British Library Electronic Table of Contents (United Kingdom)

Bio-inspired silver nanoparticles were synthesized with the aid of a novel, non-toxic, eco-friendly biological material namely, banana peel extract (BPE). Boiled, crushed, acetone precipitated, air-dried peel powder was used for reducing silver nitrate. Silver nanoparticles were formed when the reaction conditions were altered with respect to pH, BPE content, concentration of silver nitrate and incubation temperature. The colorless reaction mixtures turned brown and displayed UV-visible spectra characteristic of silver nanoparticles. Scanning electron microscope (SEM) observations revealed the predominance of silver nanosized crystallites after short incubation periods. When the reaction mixtures were incubated for 15 days, some micro-aggregates were also observed. Energy dispersive spectr...

2010-01-01

468

Are ecologists blind to small things? The missed stories on non-tropical seed predation on feces

British Library Electronic Table of Contents (United Kingdom)

Seed dispersal involves several complex stages that can be affected by multiple peripheral processes. Thus, a major dispersal event can be followed by secondary dispersal, which can reduce density-dependent mortality and consolidate the dispersal phase. But predation events can also follow, and predation of seeds in feces is particularly interesting because it can heavily alter the fate of seeds dispersed through vertebrate guts. Our review of 20 cases involving this kind of interaction found that research has chiefly been in tropical and subtropical areas (85% of cases), suggesting that it is a minor phenomenon at higher latitudes. However, the small size of both the seeds and predators involved may have caused this process to go unnoticed outside the tropics. We tested the presence of pr...

2010-01-01

469

An X-ray photoelectron spectroscopic study of B-N-Ti system

Amino acid substitutions in inherited albumin variants from Amerindian and Japanese populations

Composite nitrides (such as BN, TiN) are widely used in various industrial applications because of their extreme wear and corrosion resistance, thermal and electrical properties. in order to obtain composite materials with these optimal properties, it is important to elucidate whether any chemical reactions occur at nitride/metal interfaces, e.g., those involving BN-Ti/TiN. Materials of interest include the deposition by PVD of Ti and TiN on BN substrates. Some of these systems were then subjected to varying degrees of physical and thermal alteration. Detailed X-ray photoelectron spectroscopy (XPS) has therefore been rendered of these interfaces using cross-sectional display and sputter etching. Resulting structural and morphological features have been investigated with transmission electron microscopy (TEM) and X-ray diffraction (XRD). Diffusion of the nitridation, oxynitride formation and interfacial growth are of general interest.

1997-04-04

470

APC and KRAS mutations in distal colorectal polyps are related to smoking habits in men: results of a cross-sectional study

The authors report an effort to determine the basis for the altered migration of seven inherited albumin variants detected by one-dimensional electrophoresis in population surveys involving tribal Amerindians and Japanese children. An amino acid substitution has thus far been determined for four of the variants. The randomness in the albumin polypeptide of these and the other sixteen independently ascertained amino acid substitutions of albumin and proalbumin thus far established was analyzed; the clustering of eight of these at two positions in the six-amino acid propeptide sequence seems noteworthy. By comparison with other proteins studied by electrophoresis, albumin exhibits average variability. It is a paradox that individuals who, for genetic reasons, lack albumin exhibit no obvious ill effects; yet, electrophoretic variants of albumin are no more numerous than are variants of proteins, the absence of which results in severe disease.

471

British Library Electronic Table of Contents (United Kingdom)

Background The purpose of this study was (a) to evaluate the association between cigarette smoking and the prevalence of distal colorectal polyps and adenocarcinoma and (b) to analyse genetic alterations representing different molecular pathways of the colorectal carcinogenesis. Methods A total of 623 asymptomatic male (mean age: 53 years; 50?65) car factory workers were included. Information on smoking habits and other lifestyle factors were collected followed by a 60 cm colonoscopy. APC and KRAS mutations and microsatellite status were determined in colorectal lesions (colorectal carcinoma (CRC), hyperplastic (HP) and adenomatous polyps (AP)). Data were analysed using unconditional multiple logistic regression models. Results Smokers had a higher prevalence of AP (OR 2.1; 95% CI 1.2?3.6;...

2011-01-01

472

5,8,11,14-Eicosatetraynoic acid-induced destruction of mitochondria in human prostate cells (PC-3).

2010 power generation sector restructuring in Romania-A critical assessment

Culturing human prostate PC-3 cells for 4, 24, or 72 h in the presence of 5,8,11,14-eicosatetraynoic acid (ETYA), an inhibitor of arachidonic acid metabolism and cholesterol biosynthesis, markedly altered the morphology and reduced the number of mitochondria in the treated cells. Using quantitative electron microscopic morphometry, we documented changes in the number, form, area, matrix density, and integrity of the cristae and limiting membranes of mitochondria in cells cultured with ETYA. The inhibition of cholesterol synthesis or the substitution of ETYA for polyunsaturated fatty acids in the inner membrane may participate in the disruption of the mitochondria, which resembles the morphologic sequelae of oxidative stress. If sufficiently extensive, these changes could contribute to the inhibition of cellular proliferation by ETYA.

1992-01-01

473

1983 Northern Australia mine rehabilitation workshop - papers presented

In the spring of 2009, the Romanian government has proposed a restructuring of the national power generation sector segment that is still owned by the state. As most of the power generation capacity is still state-owned, this restructuring will dramatically alter the competitive landscape of this sector. This paper is analyzing the rationale and principles of the restructuring as stated by government documents and officials, the assessment methodology and criteria proposed by the government, as well as the proposed final outcome of the restructuring initiative. Based on this critical assessment and the early results of the restructuring's implementation, we are able to make recommendations that can be considered for a revised restructuring approach. - Research highlights: #-># Power generation sector restructuring is proposed in 2009 by the Romanian government. #-># We assess the objectives, methodology and proposed outcome of the restructuring. #-># ...

2011-03-01

474

Thermal action of 2. 45 GHz microwaves on the cytoplasm of Chinese hamster cells

Papers presented at the workshop include: the longterm viability of mined land in Queensland; the determination and achievement of rehabilitation objectives: a state government objective; monitoring native vegetation in regenerated bauxite mines at Weipa: a belt transect method; rehabilitation strategies at the Collinsville open cut coal mine; the alteration of the environment by the construction and operation of the coal export facility at Abbot Point; some aspects of the premining environment at Collinsville; the prospects for long term revegetation of acid coal mine spoil and reject materials; recovery and stability in disturbed and artificially revegetated plant communities: a theoretical viewpoint; revegetation in the arid tropics: Mount Isa Mines' experience; Nabralek and its rehabilitation; rehabilitation and management strategies for acid mine wastes; the purpose and design of water quality monitoring networks at mine sites; Worsley alumina project ...

1983-01-01

475

The thorny path linking cellular senescence to organismalaging

In order to demonstrate possible specific effects of microwaves at the cellular level V-79 Chinese hamster cells were exposed to 2.45-GHz radiation at power levels of 20-200 mW/cm2 and at specific absorption rates of 10-100 mW/g. Intracellular cytoplasmic changes were observed by fluorescence polarization using a method based on the intracellular enzymatic hydrolysis of nonfluorescent fluorescein diacetate (FDA). At levels of absorbed energy below 90 J/g, modifications of microviscosity and mitochondrial state were absent, but a slight stimulation of enzymatic hydrolysis of FDA was observed which may be explained by microwave-induced alterations of cellular membranes possibly due to differences in heating pattern of microwaves compared to water-bath heating. At levels of absorbed energy above 90 J/g, the decrease of enzymatic hydrolysis of FDA, increase in degree of polarization, and increase of permeation of the fluorescent marker correlated well with the decrease ...

1984-01-01

476

Study of the effect of gamma radiation on the molecule of tetracycline concerning its behavior as complexing and extracting agent; Estudo do efeito da radiacao gama sobre a molecula de tetraciclina relativamente ao seu comportamento como agente complexante e extrator

Half a century is fast approaching since Hayflick and colleagues formally described the limited ability of normal human cells to proliferate in culture (Hayflick and Moorhead, 1961). This finding--that normal somatic cells, in contrast to cancer cells, cannot divide indefinitely--challenged the prevailing idea that cells from mortal multicellular organisms were intrinsically ''immortal'' (Carrell, 1912). It also spawned two hypotheses, essential elements of which persist today. The first held that the restricted proliferation of normal cells, now termed cellular senescence, suppresses cancer (Hayflick, 1965; Sager, 1991; Campisi, 2001). The second hypothesis, as explained in the article by Lorenzini et al., suggested that the limited proliferation of cells in culture recapitulated aspects of organismal aging (Hayflick, 1965; Martin, 1993). How well have these hypotheses weathered the ensuing decades? Before answering this question, we first consider ...

2005-08-09

477

Structural Analysis for Gold Mineralization Using Remote Sensing and Geochemical Techniques in a GIS Environment: Island of Lesvos, Hellas

Both solvent extraction and spectrophotometric techniques were used to show the alterations that gamma radiation causes in the behavior of tetracycline molecule as far as its extracting and complexing power are concerned. The effect of gamma radiation on the solid tetracycline molecule, benzyl alcohol and on the solution of both was examined in solvent extraction systems whose aqueous phases were made up by {sup 152} Eu-{sup 154}Eu radioactive tracer solutions and whose organic phases were constituted by tetracycline-benzyl alcohol solutions. Experiments were performed in order to determine whether or not the water used for the pre-saturation of benzyl alcohol would influence the radiolysis of tetracycline. Solvent extraction and spectrophotometry were the techniques used to obtain the necessary data. Absorption spectra of irradiated tetracycline benzyl alcohol solutions submitted to several gamma radiation doses were examined and the ...

1982-07-01

478

Saturated bonds and anomalous electronic transport in transition-metal aluminides

Exploration for epithermal Au has been active lately in the Aegean Sea of the eastern Mediterranean Basin, both in the islands of the Quaternary arc and in those of the back-arc region. The purpose of this study was the structural mapping and analysis for a preliminary investigation of possible epithermal gold mineralization, using remotely sensed data and techniques, structural and field data, and geochemical information, for a specific area on the Island of Lesvos. Therefore, Landsat-TM and SPOT-Pan satellite images and the Digital Elevation Model (DEM) of the study area were processed digitally using spatial filtering techniques for the enhancement and recognition of the geologically significant lineaments, as well as algebraic operations with band ratios and Principal Component Analysis (PCA), for the identification of alteration zones. Statistical rose diagrams and a SCHMIDT projection Stereo Net were generated from the lineament maps and the collected field ...

2000-12-01

479

Response of a non-Hodgkin lymphoma to "6"0Co therapy monitored by 31P MRS in situ

This thesis deals with the special electronic properties of the transition-metal aluminides. Following quasicrystals and their approximants it is shown that even materials with small elementary cells exhibit the same surprising effects. So among the transition-metal aluminides also semi-metallic and semiconducting compounds exist, although if they consist of classic-metallic components like Fe, Al, or Cr. These properties are furthermore coupled with a deep pseusogap respectively gap in the density of states and strongly covalent bonds. Bonds are described in this thesis by two eseential properties. First by the bond charge and second by the energetic effect of the bond. It results that in the caes of semiconducting transition-metal aluminides both a saturation of certain bonds and a bond-antibond alteration in the Fermi level is present. By the analysis of the near-order in form of the so-calles coordination polyeders it has been succeeded to establish a simple ...

2006-05-22

480

Relationship of DNA repair processes to mutagenesis and carcinogenesis in mammalian cells. Progress report, November 1, 1979-October 31, 1980

High quality "3"1P MR spectra (signal to noise ratio (S/N) approximately 18, 15 min acquisition for each spectrum) were consistently obtained with surface coils over a period of 6-week RT. Both transient and steady state alterations in metabolites in response to RT were found in this case. The transient changes occurred during the first 3 hr immediately after the 3rd fractionated RT, these changes include the transient elevation of the PCr resonance, a decrease in PDE and an increase in intracellular pH. The monitoring showed that the metabolites approached steady state approximately 2 hr after the fractionated radiation intervention, suggesting that in vivo MRS can be useful for studying the dynamics of tumor response to RT such as repair of potential lethal damage, growth delay, and reoxygenation etc. The steady-state MR spectra showed the net response to each intervention and can clinically be useful for predicting and measuring the result of the fractionated ...

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

481

Reintrusion of silicic magma chambers by mafic dike complex: evidence from the northern Semail ophiolite

The objective of this research is to determine the role of DNA repair in mutagenesis and carcinogenesis in mammalian cells. Use of the host-cell reactivation viral suicide enrichment procedure was initiated in the isolation of repair-deficient mutants. Lightly mutagenized BHK cells were infected with irradiated Herpes simplex virus (HSV); several radiation-sensitive strains were isolated among the survivors of the infection. The characterization of these strains is progressing and the enrichments are continuing. That alterations in the frequency of mutation of C3H/10T 1/2 cells, occurring as a result of holding the cells in a confluent state following treatment with ethylmethane sulfonate, parallel the alterations in the frequency of neoplastic transformation was found. The repair capabilities of BHK cells were found to be intermediate in comparison to repair-proficient and -deficient human cells with regard to the reactivation of HSV treated ...

482

Processing of asparagine-linked oligosaccharides in HT-29 cells is a function of their state of enterocytic differentiation: an accumulation of Man/sub 9-8/-GlcNAc_2-Asn species is indicative of an impaired N-glycan trimming in undifferentiated cells

Late plagiogranite bodies in the Semail ophiolite have been previously suggested to represent late stage fractionates within an episodic spreading center magma chamber or the roots of seamount chains. Field and lab observations suggest that these late silicic magma chambers represent zones of repeated injection by dikes of intermediate to mafic composition. Multiple generations of intrusion, partial resorption and reintrusion are preserved in the plagiogranite as 1) relict phantom xenoliths, 2) angular xenoliths with quartz-rich margins, 3) deformed fine-grained dikes with distinct chilled margins, and 4) planes of rectangular blocks with cuspate margins or ellipsoids of similar fine grained mafic materials. The blocks and ellipsoids are actually dismembered mafic dikes that chilled by intruding a cooler silicic liquid and were either thermally fractured or pinched out. All of the dikes are hydrothermally altered to assemblages including amph., qtz., epi., preh., ...

1985-01-01

483

Occupational exposure to benzene: a prevention program for employees and contractors; PPEOB - Programa de Prevencao a Exposicao Ocupacional ao Benzeno para Empregados Proprios e Contratados

Studies on the regulation of the enterocytic differentiation of the human colon cancer cell line HT-29, which is differentiated in the absence (Glc"-) but not in the presence of glucose (Glc"+), have recently shown that the post-translational processing of sucrase-isomaltase and particularly its glycosylation vary as a function of cell differentiation. Other studies indicate that in undifferentiated HT-29 Glc"+ cells there is an accumulation of UDP-N-acetylhexosamine, which is involved in the glycosylation process. The purpose of the present work is to investigate whether an overall alteration of protein glycosylation is associated with the inability of HT-29 cells to differentiate. At least three alterations are detected: (i) after a 10-min pulse, the incorporation of D-[2-"3H]mannose in undifferentiated cells is severely reduced, compared to differentiated cells. (ii) After a 24-h period of labeling with D-[2-"3H]mannose, undifferentiated ...

484

Monte Carlo simulation of electron cones used in electron beam therapy

PETROBRAS/TRANSPETRO Pipelines and Terminals has 500 regular employees and 5.064 out sourced workers in its Southeast Division. The out sourced employees work through 125 contracts involving a wide range of activities such as maintenance, operational, pipeline launching , engineering, administrative and auxiliary services. Among these workers, 200 people are subjected to benzene occupational exposure, which might be present in the products we transport in our pipelines. Benzene is recognized as a carcinogen according to ACGIH and Brazilian Ministry of Labour regulation NR- 15. Exposure to benzene in an uncontrolled way, be it chronic or sharp, may affect the worker's health such as: hematological alterations, neoplasys, neurobehavior alterations. Our program PPEOB (acronym in Portuguese for benzene occupational exposure prevention program) involved the work force and fosters health by anticipation, recognition, evaluation and control ...

2004-07-01

485

Insulin regulation of protein biosynthesis in differentiated 3T3 adipocytes. Regulation of glyceraldehyde-3-phosphate dehydrogenase

A Siemens Mevatron KV2 accelerator installed at the Royal Adelaide Hospital employs cylindrical solid-walled electron cones for some electron collimation. The cones being used at present result in treatment fields that do not always conform with the International Electrotechnical Commission (IEC) Standards (particularly at high energies). The aim of this project was to simulate the existing cones using Monte Carlo methods in order to evaluate potential cone modifications required to overcome the field irregularities. Simulations were performed using the EGS4 (Electron Gamma Shower version 4, distribution II) Monte Carlo code installed on a DEC Alpha workstation at the University of South Australia. To rigorously simulate the existing electron cones it was necessary to also simulate various components within the treatment head of the linear accelerator. Results of simulations for existing cones were found to be consistent with experimental data. (obtained from Royal Adelaide Hospital ...

1998-09-01

486

Induction of biotransformation in the liver of Eel (Anguilla anguilla L. ) by sublethal exposure to dinitro-o-cresol: An ultrastructural and biochemical study

The effect of insulin on protein biosynthesis was examined in differentiated 3T3-L1 and 3T3-F442A adipocytes. Insulin altered the relative rate of synthesis of specific proteins independent of its ability to hasten conversion of the fibroblast (preadipocyte) phenotype to the adipocyte phenotype. Although more than one pattern of response to insulin was observed, the authors focused on the induction of a Mr 33,000 protein which was identified as the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Exposure of 3T3 adipocytes to insulin throughout differentiation specifically increased GAPDH activity and protein content by 2- to 3-fold as compared to 3T3 adipocytes differentiated in the absence of insulin. These changes in enzyme activity and content could be accounted for by a 4-fold increase in the relative rate of synthesis of GAPDH and a 9-fold increase in hybridizable mRNA levels. Within 2 h of insulin addition to 3T3 adipocytes differentiated ...

1985-10-05

487

Fractionation of combined heat and radiation in asynchronous CHO cells. II. The role of cell-cycle redistribution

Structural and functional alterations in hepatocytes of the European eel, Anguilla anguilla, following a 4-week-exposure to 5, 50, and 250 micrograms/liter dinitro-o-cresol (DNOC) were investigated by means of electron microscopy and biochemistry and compared to liver pathology in eels exposed to the chemical spill into the Rhine river at Basle in November 1986. Whereas phenological parameters (growth, condition factor) are unaffected, ultrastructural and biochemical alterations are detectable at greater than or equal to 50 and 5 micrograms/liter DNOC, respectively. Structural modifications include: rounding-up of the nuclei; fractionation and reduction of the rough endoplasmic reticulum; proliferation of the smooth endoplasmic reticulum (SER), mitochondria, peroxisomes, and lysosomes; bundles of rod-shaped SER profiles; annulate lamellae; membrane whorls within mitochondria; crystallization of the peroxisomal matrix and glycogen bodies; ...

1991-04-01

488

Effects of chronic swimming training on cardiac sarcolemmal function and composition.

Asynchronous Chinese hamster ovary (CHO) cells treated with conditioning hyperthermia (10 min at 45/sup 0/C) followed immediately by x irradiation with 4 Gy at room temperature display a decreased radiation response when either irradiated, or heated and irradiated again 24 h later. In addition, conditioning by 4 Gy of x radiation alone followed at 24 h by the combined heat and radiation treatment als resulted in a larger radiation D/sub 0/. Since the differential cell-cycle responses to heat- and x-ray-induced lethality are well established for CHO cells, the potential role of cell-cycle redistribution in the observed radiation desensitization was investigated by flow cytometry. At 24 h after the combined treatment, the fraction of the cells found in the G/sub 2/ + M portion of the DNA histograms was about three times that of the controls and there was a concomitant reduction in both the G/sub 1/ and S fractions. Similar observations were made after the conditioning heat treatment ...

1979-11-01

489

Climate change, agriculture and wetlands in Eastern Europe: vulnerability, adaptation and policy

Cardiac contractile function is dependent on the integrity and function of the sarcolemmal membrane. Swimming exercise training is known to increase cardiac contractile performance. The purpose of the present study was to examine whether a swimming exercise program would alter sarcolemmal enzyme activity, ion flux, and composition in rat hearts. After approximately 11 wk of exercise training, cardiac myosin and actomyosin Ca2+-adenosinetriphosphatase (ATPase) activity was significantly higher in exercised rat hearts than in sedentary control rat hearts. Glycogen content was increased in plantaris and gastrocnemius muscles from exercised animals as was succinic dehydrogenase activity in gastrocnemius muscle of exercised rats in comparison to sedentary rat preparations. Sarcolemmal vesicles were isolated from hearts of exercise-trained and control rats. Sarcolemmal Na+-K+-ATPase and K+-p-nitrophenylphosphatase activities, Na+-Ca2+ exchange, and passive Ca2+ binding ...

1989-04-01

490

Chemical Makeup of Microdamaged Bone Differs from Undamaged Bone

Naturally-occurring wetlands perform such functions as flood control, pollution filtration, nutrient recycling, sediment accretion, groundwater recharge and water supply, erosion control, and plant and wildlife preservation. A large concentration of wetlands is located in Eastern Europe. A significant amount of Eastern European wetlands has been converted to agricultural use in the past, and remaining wetlands are subject to agricultural drainage. Drained wetlands are used as prime agriculture lands for a variety of food crops. Other agricultural uses of wetlands range from growing Phragmites australis (common reed) for thatch and livestock feed, to collecting peat for heating and cooking fuel. Altered hydrologic regimes due to global climate change could further exacerbate encroachment of agricultural land use into wetlands. The vulnerability and adaptation studies of the US Country Studies Program are used to analyze where climate change impacts to agriculture ...

1997-05-01

491

Behaviour of silicon released during alteration of nuclear waste glass in compacted clay

Microdamage naturally occurs in bone tissue as a result of cyclic loading placed on the body from normal daily activities. While it is usually repaired through the bone turnover process, accumulation of microdamage may result in reduced bone quality and increased fracture risk. It is unclear whether certain areas of bone are more susceptible to microdamage than others due to compositional differences. This study examines whether areas of microdamaged bone are chemically different than undamaged areas of bone. Bone samples (L3 vertebrae) were harvested from 15 dogs. Samples were stained with basic fuchsin, embedded in poly-methylmethacrylate, and cut into 5-(micro)m-thick sections. Fuchsin staining was used to identify regions of microdamage, and synchrotron infrared microspectroscopic imaging was used to determine the local bone composition. Results showed that microdamaged areas of bone were chemically different than the surrounding undamaged areas. Specifically, the mineral ...

2006-01-01

492

Altered lauric acid metabolism in renal microsomes from spontaneously hypertensive rats (SHR)

Long term integrated in situ experiments are performed in the HADES underground research facility (Mol, Belgium) in order to study the coupled reactivity between the different components of an underground repository for vitrified high level radioactive waste (HLW): glass, compacted clay, and stainless steel containers, at 90 degrees C and under gamma irradiation. Studies pertaining to the behaviour of silicon, a major element released during glass alteration, are presented here. Data collected from the integrated experiment, from simplified tests, and from modelling are put together, giving complementary information. The integrated experiment is used to investigate overall reactivity, whereas diffusion experiments coupled with modelling focused on the precipitation of silica in clay media. In the integrated in situ experiment, a bentonite clay (FoCa7) mixed with 5 wt.% of powdered glass frit was put in contact with U/Th-doped SON68 reference glass specimens for 1.2 ...

2007-02-15

493

A PIXE/PIGE study of gold mineralisation in lateritic terrain, Tanami Desert, Australia

Studies investigated whether changes in omega- and (omega-1)-hydroxylation (OH) of lauric acid (LA) occurred in renal microsomes prepared from SHR compared to Wistar-Kyoto (WK) control rats. Systolic blood pressure in age-matched adult SHR and WKR were 189 +/- 3 and 123 +/- 4 mm Hg(anti X +/- SE) respectively (p < 0.001). No significant differences between SHR and WKR were seen in body weight, kidney weight or renal microsomal protein content. Renal microsomes, prepared from whole kidneys, were incubated with 10 mM NADPH and ["1"4C]LA at concentrations between 5-50 #mu#M. The 11- and 12-OH metabolites of LA were separated by HPLC using a reverse phase column with a methanol:water:acetic acid (62:37.8:0.2) mobile phase. Apparent (app) V/sub max/ values for 12-OH in WKR and SHR were 0.87 +/- 0.19 vs 1.48 +/- .11 nmoles/mg protein/min (p < 0.05), respectively, while values for 11-OH were 0.51 +/- 0.12 vs 0.60 +/- .07, respectively. No significant differences were found in app K/sub ...

1986-04-13

494

The radiation hardening and microstructural defect evolution in ion irradiated Fe-Cr alloys with irradiation temperature

Proton induced X-ray and {gamma}-ray emission (PIXE/PIGE) have been used to analyze major and trace elements in a suite of 140 core samples from around of the Jim`s Find South gold anomaly in the Tanami desert, located in heavily weathered terrain. Simultaneous analyses were obtained for 30 elements, ranging in atomic number from {sup 3}Li to {sup 90}Th. The method was chosen because of its speed and the wide range of determination, its flexibility, precision and low detection limits. The regolith powder samples were treated by hot aqua regia before making them into pills. The PIXE/PIGE data of the acid insoluble residue give three factor analysis clusters. The first cluster comprises the elements F, Al, K, V, Mn, Fe, Ga, Rb, W and Au and is essentially related to sericitic wallrock alteration. The second cluster consists of Ti, As, Y, Zr, and Nb and is largely related to resistant minerals. The third cluster consists of Na, Ca and Sr and is interpreted to comprise ...

1997-12-31

495

Mechanisms of renal handling of lead

Generally, neutron, ion and electron Irradiations cause a substantial amount of hardening and significantly alter the deformation behavior of metals and alloys at relatively low irradiation temperatures. A radiation hardening is caused by the formation of microstructural defects such as dislocation loops, voids and precipitates under irradiation. Therefore, it is important to have a better knowledge of the irradiation induced microstructural defects under irradiation condition. As a part of the National mid- and long-term atomic energy R and D program, we are dealing with the radiation hardening behavior in Fe-Cr binary alloy. Fe-Cr binary alloy is a base alloy of Ferritic/Martensitic steel(F/M steel) planning to use for the Gen IV nuclear system. In this work, we investigated the radiation hardening and microstructural defect evolution in ion irradiated Fe-Cr alloys with irradiation temperature using nano-indentation measurement and transmission electron ...

2009-05-01

496

Magnetic resonance imaging: early detection of central nervous system involvement in acquired immunodeficiency syndrome (AIDS)

The relative roles of glomerular filtration, tubular reabsorption and tubular secretion in lead handling were evaluated using renal clearance technique, stop-flow analysis, and renal lead accumulation. In addition, the effect of altering acid-base status on renal handling of Pb was determined. Clearance of ultrafilterable lead was measured in anesthetized dogs during infusion of "2"0"3Pb with small doses of carrier Pb(0.5 to 50 #mu#g/kg/h). Filterable lead concentration was estimated by in vitro ultrafiltration of non-anticoagulated plasma. Fractional excretion of Pb was calculated using creatinine clearance to estimate glomerular filtration rate. Dogs in acute metabolic acidosis had the lowest fractional excretion of ultrafilterable Pb (< 0.1); those in alkalosis had high fractional Pb excretion (0.75 to 2.5) while normal dogs were intermediate (0.1 to .4). The fractional excretion of Pb during metabolic alkalosis varied with the dose of Pb administered; only ...

497

In vivo evaluation of a lead-labeled monoclonal antibody using the DOTA ligand

Central Nervous System (CNS) involvement, whether primary by the Human Immunodeficiency Virus - HIV - itself, or secondary (toxoplasmosis or lymphoma) is remarkably frequent in AIDS, in 40 to 70% of cases, depending upon the author. In order to study the natural history of this illness, a cohort of 25 asymptomatic seropositive patients have been established. Every 6 months these patients undergo biological and clinical examinations, as well as Magnetic Resonance brain scans. After two examinations at a 6 month's interval, the first results are reported. Out of these 25 cases, 9 present anomalies: One patient with diffuse cerebral atrophy and 8 others with high signal intensity areas on T2 weighted sequences, like those of the Multiple Sclerosis. No relationship could be demonstrated between the existence of these lesions and various criteria such as age, sex, risk factors and T4 cells count. The nature of these lesions is not lear. They certainly indicate early involvement of the CNS ...

498