Earlier studies have indicated that xylitol may increase the absorption and urinary excretion of dietary oxalate. It has also been indicated that xylitol increases the absorption of calcium. Intestinal absorption of lead, a divalent contaminant in the diet, is in many respects similar to that of calcium. The purpose of this study was to evaluate the effects of xylitol on the intestinal absorption of lead using two different approaches: the in situ ligated intestinal loop technique in cockerels and gastric gavage in mice.
Neutralized hydrolysate and pretreated rice straw obtained from a 2% (w/v) sulfuric acid pretreatment were mixed at 10% (w/v) and subjected to simultaneous saccharification and co-fermentation (SSCF), with cellulase, b-glucosidase, and Candida tropicalis cells at 15FPU/g-ds, 15IU/g-ds and 1x10^9cells/ml, respectively. A 36-h SSCF with adapted cells resulted in YP/S and ethanol volumetric productivity of 0.36g/g and 0.57g/l/h, respectively. In addition to ethanol, insignificant amounts of glycerol and xylitol were also produced. Adapted C. tropicalis cells produced nearly 1.6 times more ethanol than non-adapted cells. Ethanol yield (Yp/s), ethanol volumetric productivity and a xylitol concentration of 0.48g/g, 0.33g/l/h and 0.89g/l, respectively, were produced from fermentation of remaining...
Methods were developed for the separation of radioactively labeled carbohydrate components of proteoglycans by isocratic ion-moderated partition HPLC. Neutral sugars were separated after hydrolysis in trifluoroacetic acid with baseline separation between glucose, xylose, galactose, fucose, and mannose. N-Acetylneuraminic acid, N-acetylated hexosamines, glucose, galactose, and xylitol were likewise well separated from each other under isocratic elution conditions. Glucuronic acid, iduronic acid, and their lactones were separated after hydrolysis in formic acid and sulfuric acid. Glucosamine, galactosamine, galactosaminitol, and glucosaminitol were separated by HPLC on a cation exchanger with neutral buffer after hydrolysis in hydrochloric acid. THe separation techniques also proved useful in fractionation of exoglycosidase digests of O- and N-linked oligosaccharides. Separations of aldoses, hexosamines, and uronic acids were adapted to sensitive photometric ...