variant macroh2a1 deletion: Topics by WorldWideScience.org

Sequence analysis of the ATM gene in 20 patients with RTOG grade 3 or 4 acute and/or late tissue radiation side effects

Copy number variants (CNVs) are an important component of genomic variation in humans and other mammals. Similar de novo deletions and duplications, or copy number changes (CNCs), are now known to be...Full Text Available

2009-03-13

Retrograde neuronal tracing with a deletion-mutant rabies virus

Purpose: Patients with ataxia-telangiectasia (A-T) show greatly increased radiation sensitivity and cancer predisposition. Family studies imply that the otherwise clinically silent heterozygotes of this autosomal recessive disease run a 3.5 to 3.8 higher risk of developing cancer. In vitro studies suggest moderately increased cellular radiation sensitivity of A-T carriers. They may also show elevated clinical radiosensitivity. We retrospectively examined patients who presented with severe adverse reactions during or after standard radiation treatment for mutations in the gene responsible for A-T, ATM, considering a potential means of future identification of radiosensitive individuals prospectively to adjust dosage schedules. Material and Methods: We selected 20 cancer patients (breast, 11; rectum, 2; ENT, 2; bladder, 1; prostate, 1; anus, 1; astrocytoma, 1; Hodgkins lymphoma, 1) with Grade 3 to 4 (RTOG) acute and/or late tissue radiation side effects by reaction severity. DNA from the ...

1999-07-15

Replication of Extended Lifespan Phenotype in Mice with Deletion of Insulin Receptor Substrate 1

We have constructed a deletion-mutant rabies virus encoding EGFP and find it to be an excellent tool for studying detailed morphology and physiology of neurons projecting to injection sites...Full Text Available

2007-01-01

Deletion of the Receptor for Advanced Glycation End Products Reduces Glomerulosclerosis and Preserves Renal Function in the Diabetic OVE26 Mouse

We previously reported that global deletion of insulin receptor substrate protein 1 (Irs1) extends lifespan and increases resistance to several age-related pathologies in female mice....Full Text Available

Conditional deletion of Abca3 in alveolar type II cells alters surfactant homeostasis in newborn and adult mice

OBJECTIVEPrevious studies showed that genetic deletion or pharmacological blockade of the receptor for advanced glycation end products (RAGE) prevents the early structural changes...Full Text Available

2010-08-01

WIS (WWMCCS (Worldwide Military Command and Control ...

ATP-binding cassette A3 (ABCA3) is a lipid transport protein required for synthesis and storage of pulmonary surfactant in type II cells in the alveoli. Abca3 was conditionally deleted...Full Text Available

2010-05-01

Recurrent miscarriage and variant alleles of mannose binding lectin, tumour necrosis factor and lymphotoxin ? genes

... the print queue, changing the priority of print jobs, -deleting a job from a print queue, aborting a ... "standard terminal interface" should ... f. Stochastic 27 ...

1985-07-01

Persistence of genetic variants of the arctic fox strain of Rabies virus in southern Ontario

Variant alleles of the mannose binding lectin (MBL) gene are associated with increased susceptibility to infection and polymorphisms of tumour necrosis factor and lymphotoxin alpha genes (TNF, LTA)...Full Text Available

2001-12-01

Multiorgan Detection and Characterization of Protease-Resistant Prion Protein in a Case of Variant CJD Examined in the United States

AbstractGenetic-variant analysis of rabies viruses provides the most sensitive epidemiologic tool for following the spread and persistence of these viruses in their wildlife hosts. Since...Full Text Available

2006-01-01

Molecular characterization of a Chinese variant of the Flury-LEP strain

BackgroundVariant Creutzfeldt–Jakob disease (vCJD) is a prion disease thought to be acquired by the consumption of prion-contaminated beef products. To date, over 200 cases...Full Text Available

A Computational Framework Discovers New Copy Number Variants with Functional Importance

The entire genome of rabies virus vaccine strain Flury-LEP-C, a Chinese variant of the rabies virus vaccine strain Flury-LEP, was sequenced. The overall length of the genome of Flury-LEP-C strain was...Full Text Available

[Comparison of HbA(1c) Analyzers: D-10, Variant II Turbo, Cobas Integra 800, and Afinion AS100.

Structural variants which cause changes in copy numbers constitute an important component of genomic variability. They account for 0.7% of genomic differences in two individual genomes, of which...Full Text Available

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2010-08-01

Differential regulation of iPLA2beta splice variants by in vitro ischemia in C2C12 myotubes

Infinitely many nontrivial solutions for a class of biharmonic equations via variant fountain theorems

Udgivelsesdato: March

2006-01-01

British Library Electronic Table of Contents (United Kingdom)

By a variant version of Fountain Theorem due to Zou [W. Zou, Variant fountain theorems and their applications, Manuscripta Math. 104 (2001) 343-358], the existence of infinitely many solutions is obtained for a class of biharmonic equations where the nonlinearity involves a combination of superlinear and asymptotically linear terms.

2011-01-01

Amino acid substitutions in inherited albumin variants from Amerindian and Japanese populations

Crystallography of grain boundary #alpha# precipitates in a #beta# titanium alloy

The authors report an effort to determine the basis for the altered migration of seven inherited albumin variants detected by one-dimensional electrophoresis in population surveys involving tribal Amerindians and Japanese children. An amino acid substitution has thus far been determined for four of the variants. The randomness in the albumin polypeptide of these and the other sixteen independently ascertained amino acid substitutions of albumin and proalbumin thus far established was analyzed; the clustering of eight of these at two positions in the six-amino acid propeptide sequence seems noteworthy. By comparison with other proteins studied by electrophoresis, albumin exhibits average variability. It is a paradox that individuals who, for genetic reasons, lack albumin exhibit no obvious ill effects; yet, electrophoretic variants of albumin are no more numerous than are variants of proteins, the ...

The adenovirus-2 EIIa early gene promoter: sequences required for efficient in vitro and in vivo transcription.

The crystallography of #alpha#(hcp) precipitates formed on the #beta#(bcc) matrix grain boundaries has been studied with transmission electron microscopy (TEM) in a Ti-15V-3Cr-3Sn-3Al alloy. The #alpha# precipitates have a near-Burgers orientation relationship with respect to at least one of the adjacent #beta# grains. Among the possible 12 variants in this orientation relationship, the variant that [11 bar 20]_#alpha# is parallel to the _#beta# closest to the grain boundary plane tends to be preferred by the #alpha# precipitates. Additionally, further variant selections are made so as to minimize the deviation of orientation relationship with respect to the ''opposite'' #beta# grain from the Burgers one. Such rules in variant selection often result in the formation of precipitates with a single variant at a planar grain boundary. Prior small deformation of #beta# matrix changes the ...

1994-10-03

The Case for Selection at CCR5-?32

A series of deletion mutants extending from -250 toward the capsite has been constructed in the early promoter region of the adenovirus 2 EIIa gene and tested both in vitro, and in vivo after transfection...Full Text Available

1983-10-25

Targeted Deletion of Somatotroph Insulin-Like Growth Factor-I Signaling in a Cell-Specific Knockout Mouse Model

The C-C chemokine receptor 5, 32 base-pair deletion (CCR5-Δ32) allele confers strong resistance to infection by the AIDS virus HIV. Previous studies have suggested...Full Text Available

2005-11-01

Rai1 duplication causes physical and behavioral phenotypes in a mouse model of dup(17)(p11.2p11.2)

The role of IGF-I in the negative regulation of GH expression and release is demonstrated by in vitro and in vivo models; however, the targets and mechanisms of IGF-I...Full Text Available

2010-05-01

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RXPERT: A Decision Support Expert System for Drug Product Interchangeability Assessment

Genomic disorders are conditions that result from DNA rearrangements, such as deletions or duplications. The identification of the dosage-sensitive gene(s) within the rearranged genomic interval is...Full Text Available

2006-11-01

Natural variation in life history and aging phenotypes is associated with mitochondrial DNA deletion frequency in Caenorhabditis briggsae

A knowledge-based expert system has been developed to support human decision makers who assess drug products for inclusion in the Saskatchewan Formulary. Formulary inclusion and deletion decisions...Full Text Available

1989-11-08

Identification of copy number variations and common deletion polymorphisms in cattle

BackgroundMutations that impair mitochondrial functioning are associated with a variety of metabolic and age-related disorders. A barrier to rigorous tests of the role of mitochondrial...Full Text Available

Evidence for increased in vitro recombination with insertion of human hepatitis B virus DNA.

BackgroundRecently, the discovery of copy number variation (CNV) led researchers to think that there are more variations of genomic DNA than initially believed. Moreover, a certain...Full Text Available

Deletion of the Plasmodium falciparum Merozoite Surface Protein 7 Gene Impairs Parasite Invasion of Erythrocytes?

Chromosomal translocation, deletion, and inversion/duplication directly linked to hepatitis B virus (HBV) DNA integration occur frequently in host DNA of human hepatocellular carcinomas. To test the...Full Text Available

1991-10-15

Deletion of TolC orthologs in Francisella tularensis identifies roles in multidrug resistance and virulence

Merozoite surface proteins have been implicated in the initial attachment to the host red blood cell membrane that begins the process of invasion, an important step in the life cycle of the malaria...Full Text Available

2008-12-01

Chromatography Equipment on Environmental Expert

The Gram-negative bacterium Francisella tularensis is the causative agent of tularemia. Interest in this zoonotic pathogen has increased due to its classification as a category A agent...Full Text Available

2006-08-22

Wastenet

...for free Subscribe your products Categories All Categories Monitoring & Testing ChromatographyDelete Category Filter anion exchange chromatography biogas chromatographs chromatographic media chromatographic separations chromatographs chromatography ...

Bunyamwera virus can repair both insertions and deletions during RNA replication

Angiotensin II Promotes Development of the Renal Microcirculation through AT₁ Receptors

The genomic termini of RNA viruses contain essential cis-acting signals for such diverse functions as packaging, genome translation, mRNA transcription, and RNA replication, and thus...Full Text Available

2010-06-01

Alterations in the steroid hormone receptor co-chaperone FKBPL are associated with male infertility: a case-control study

Pharmacologic or genetic deletion of components of the renin-angiotensin system leads to postnatal kidney injury, but the roles of these components in kidney development are unknown. To test the hypothesis...Full Text Available

2010-03-01

A novel custom high density-comparative genomic hybridization array detects common rearrangements as well as deep intronic mutations in dystrophinopathies

BackgroundMale infertility is a common cause of reproductive failure in humans. In mice, targeted deletions of the genes coding for FKBP6 or FKBP52, members of the FK506 binding...Full Text Available

A new evolutionary scenario for the Mycobacterium tuberculosis complex

BackgroundThe commonest pathogenic DMD changes are intragenic deletions/duplications which make up to 78% of all cases and point mutations (roughly 20%) detectable...Full Text Available

A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.

The distribution of 20 variable regions resulting from insertion-deletion events in the genomes of the tubercle bacilli has been evaluated in a total of 100 strains of Mycobacterium tuberculosis,...Full Text Available

2002-03-19

The quasimolecular model in transitional nuclei

We have previously shown that a T to G transversion at the second T of the conalbumin "TATA" box drastically decreases specific initiation of transcription by RNA polymerase B (1). We now report that...Full Text Available

1981-04-24

Pneumomediastinum as a complication of emphysematous cholecystitis: Case report

A review is made of some recent developments in the application of the Bohr Hamiltonian to transitional nulcei. The primary concern is to understand the advantages and limitations inherent to the basic model and its many variants. (author).

A Study of Avionics Time Division Multiplex Bus Simulation.

BackgroundEmphysematous cholecystitis is a variant of acute cholecystitis which is generally caused by gas-forming organisms. Emphysematous cholecystitis may cause gas spreading...Full Text Available

Revisiting new variant famine: the case of Swaziland

... which may have embedded remote terminals, and (4 ... model the time-variant or stochastic aspects of ... on such system parameters as queue size, time ...

1980-12-01

British Library Electronic Table of Contents (United Kingdom)

The ?new variant famine? hypothesis posits links between HIV/AIDS and new patterns of impoverishment, food insecurity and hunger, in southern and eastern Africa. This paper explores the relevance of the NVF hypothesis to understanding Swaziland?s recurrent food crises and high HIV prevalence. Evidence exists that all four markers of NVF are present in Swaziland. The national government and the international community will have to contend with this phenomenon in future planning for the wellbeing of Swazi citizens.

2009-01-01

New Variant of CTX-M-Type Extended-Spectrum ?-Lactamases, CTX-M-71, with a Gly238Cys Substitution in a Klebsiella pneumoniae Isolate from Bulgaria?

Meta-Analysis for Genome-Wide Association Study Identifies Multiple Variants at the BIN1 Locus Associated with Late-Onset Alzheimer's Disease

A single Klebsiella pneumoniae strain isolated in a Bulgarian hospital was found to produce CTX-M-71, a new CTX-M variant characterized by one amino acid substitution...Full Text Available

2009-10-01

Antigenic drift in influenza virus H3 hemagglutinin from 1968 to 1980: multiple evolutionary pathways and sequential amino acid changes at key antigenic sites.

Recent GWAS studies focused on uncovering novel genetic loci related to AD have revealed associations with variants near CLU, CR1, PICALM and BIN1....Full Text Available

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Analysis of the 10q11 Cancer Risk Locus Implicates MSMB and NCOA4 in Human Prostate Tumorigenesis

Surveys of the antigenic properties of a wide range of variants of the H3N2 (Hong Kong) influenza virus subtype have revealed complex patterns of variants cocirculating during each of the main epidemic...Full Text Available

1983-10-01

Microstructural development inside the stress induced martensite variant in a Ti-Ni-Nb shape memory alloy

Genome-wide association studies (GWAS) have established a variant, rs10993994, on chromosome 10q11 as being associated with prostate cancer risk. Since the variant is located outside of a protein-coding...Full Text Available

2010-11-01

Characterization of mal recombination plasmids cloned in Streptococcus pneumoniae

The microstructural development inside the stress induced martensite (SIM) variants in Ti-Ni-Nb alloy with various degrees of deformation have been revealed by electron microscopic observations. The orientation relationship between the SIM and the parent phase has been found: [1{bar 1}0]{sub M}{parallel}[11{bar 1}]{sub B2}, (001){sub M} 5{degree} away from (101){sub B2}. The lattice invariant shear of the SIM variants at the slightly deformed stage is dominantly (11{bar 1}) Type I twin. Besides the ordinary slip, the adjustment and development of the internal secondary twinning from (11{bar 1}) Type I twin to {l_angle}011{r_angle} Type II/ or (011) Type I twin, (001)compound twin and (111) Type I twin happen concurrently or in combination inside the SIM variants with the further deformation. The corresponding deformation mechanisms include stress induced reorientation of SIM substructural bands by the most favorably ...

2000-04-03

Detection of the first gross CDC73 germline deletion in an HPT-JT syndrome family

The malM locus of Streptococcus pneumoniae was cloned into one of the two PstI sites of the multicopy S. pneumoniae plasmid pMV158. To eliminate chromosomal transformants in the simultaneous selection for tetracycline resistance (coded by pMV158) and maltose utilization, the host cells contained a chromosomal deletion of the mal gene cluster. Two clones were isolated; one with a 3.3 kb insert (pLS70) which behaved like wild type with respect to maltose utilization, and another with a 2.9 kb insert (pLS69) which behaved as though it contained a down promoter mutation. Preliminary mapping of these clones by restriction analysis placed the 0.4kb deletion on a HindIII fragment in the interior of the chromosomal insert. The recombinant plasmids were able to transform over 50% of a recipient population to Mal/sup +/. Enzyme measurements of the clones indicated an overproduction of amylomaltase, constituting up to 10% of the total cellular protein, ...

1981-01-01

British Library Electronic Table of Contents (United Kingdom)

Abstract Hereditary primary hyperparathyroidism (HPT) may develop as a solitary endocrinopathy (FIHP) or as part of multiple endocrine neoplasia Type 1, multiple endocrine neoplasia Type 2A, or hereditary HPT-jaw tumor syndrome. Inactivating germline mutations of the tumor suppressor gene CDC73 account for 14 and 50% of all FIHP and HPT-JT patients, respectively, and have also been found in almost 20% of apparently sporadic parathyroid carcinoma patients. Although more than 60 independent germline mutations have been described, to date no rearrangement affecting the CDC73 locus has been identified. By means of multiplex-PCR we found a large germline deletion affecting the whole gene in a two-generation HPT-JT family. Subsequently array-CGH and specific PCR analysis determined that the muta...

2011-01-01

Chern classes of graph hypersurfaces and deletion-contraction

Genomic architecture of aggression: Rare copy number variants in intermittent explosive disorder

We study the behavior of the Chern classes of graph hypersurfaces under the operation of deletion-contraction of an edge of the corresponding graph. We obtain an explicit formula when the edge satisfies two technical conditions, and prove that both these conditions hold when the edge is multiple in the graph. This leads to recursions for the Chern classes of graph hypersurfaces for graphs obtained by adding parallel edges to a given (regular) edge. Analogous results for the case of Grothendieck classes of graph hypersurfaces were obtained in previous work. Both Grothendieck classes and Chern classes were used to define `algebro-geometric' Feynman rules. The results in this paper provide further evidence that the polynomial Feynman rule defined in terms of the Chern-Schwartz-MacPherson class of a graph hypersurface reflects closely the combinatorics of the corresponding graph. The key to the proof of the main result is a more general formula for the ...

2011-01-01

British Library Electronic Table of Contents (United Kingdom)

Abstract Copy number variants (CNVs) are known to be associated with complex neuropsychiatric disorders (e.g., schizophrenia and autism) but have not been explored in the isolated features of aggressive behaviors such as intermittent explosive disorder (IED). IED is characterized by recurrent episodes of aggression in which individuals act impulsively and grossly out of proportion from the involved stressors. Previous studies have identified genetic variants in the serotonergic pathway that play a role in susceptibility to this behavior, but additional contributors have not been identified. Therefore, to further delineate possible genetic influences, we investigated CNVs in individuals diagnosed with IED and/or personality disorder (PD). We carried out array comparative genomic hybridizati...

2011-01-01

A generic quantum walk using a coin-embedded shift operator

Variants within the yeast Ty sequence family encode a class of structurally conserved proteins.

The study of quantum walk process has been widely divided into the two standard variants, the discrete-time quantum walk (DTQW) and the continuous-time quantum walk (CTQW). The connection between the two variants has been established by considering limiting value of the coin operation parameter in the DTQW and the coin degree of freedom is show to be unnecessary [26]. But the coin degree of freedom is an additional resource which can be exploited to control the dynamics of the QW process. In this paper we present a generic quantum walk (QW) model using a quantum coin-embedded unitary shift operation U_{C}. The standard version of the DTQW and the CTQW can be conveniently retrieved from this generic model retaining the features of the coin degree of freedom in both the variants.

2008-01-01

Variants in Inflammation Genes and the Risk of Biliary Tract Cancers and Stones: A Population-based Study in China

The Ty transposable elements of Saccharomyces cerevisiae form a heterogeneous family within which two broad structural classes (I and II) exist. The two classes differ by two large substitutions and...Full Text Available

1985-06-11

Variant influenza virus hemagglutinin that induces fusion at elevated pH.

To evaluate the role of chronic inflammation in the development of gallstones and biliary tract cancer, we examined the risk associated with 62 single nucleotide polymorphisms (SNPs), including...Full Text Available

2008-08-01

Transmitted drug resistance in nonsubtype B HIV-1 infection

The hemagglutinin (HA) glycoprotein of influenza virus performs two critical roles during infection: it binds virus to cell surface sialic acids, and under mildly acidic conditions it induces fusion...Full Text Available

1986-02-01

The influence of prior ageing on creep damage development in two variants of Alloy 800

HIV-1 nonsubtype B variants account for the majority of HIV infections worldwide. Drug resistance in individuals who have never undergone antiretroviral therapy can lead to early failure and...Full Text Available

2009-09-01

The Association of Alcohol and Alcohol Metabolizing Gene Variants with Diabetes and Coronary Heart Disease Risk Factors in a White Population

The influence of high temperature thermal ageing treatments on the development of intercrystalline creep damage in two variants of Alloy 800 has been investigated. Ageing up to 3000 h and creep testing were carried out at 800 and 900 C. The high temperature behaviour of the 800HT variant is discussed with reference to the effect of heat treatments on the microstructure. The metallographic methods by which the creep damage was quantitatively determined are described. The growth rate of intercrystalline microcracks was described using a statistical model and the dependence of crack growth rate on the thermal history for both 800HT and 800H was determined. The carbide precipitation and growth processes were determined as functions of the exposure temperature and duration. The results showed the three characteristic stages, precipitation, growth and coarsening (Ostwald ripening). The largest increase in the intergranular creep damage was found in ...

1997-06-01

Soluble Variants of Rhodobacter capsulatus Membrane-anchored Cytochrome c_y Are Efficient Photosynthetic Electron Carriers*

BackgroundEpidemiological studies have shown a J- or U-shaped relation between alcohol and type 2 diabetes and coronary heart disease (CHD). The underlying mechanisms are not clear....Full Text Available

Sequence variants of the DFNB31 gene among Usher syndrome patients of diverse origin

Photosynthetic (Ps) electron transport pathways often contain multiple electron carriers with overlapping functions. Here we focus on two c-type cytochromes (cyt) in facultative phototrophic...Full Text Available

2008-05-16

Properties of a cell-wall-defective variant of Brucella abortus of bovine origin.

PurposeIt has been demonstrated that mutations in deafness, autosomal recessive 31 (DFNB31), the gene encoding whirlin, is responsible for nonsyndromic hearing loss...Full Text Available

Olfactory copy number association with age at onset of Alzheimer disease

The properties of an atypical Brucella strain isolated from lymph node tissue of a cow slaughtered as a brucellosis reactor were examined. The organism was Gram negative and highly pleomorphic, existing...Full Text Available

1980-08-01

New common variants affecting susceptibility to basal cell carcinoma

Objectives:Copy number variants (CNVs) have been recognized as a source of genetic variation that contributes to disease phenotypes. Alzheimer disease (AD) has high heritability...Full Text Available

2011-04-12

Multiplicity of Plasmodium falciparum infection in asymptomatic children in Senegal: relation to transmission, age and erythrocyte variants

In a follow-up to our previously reported genome-wide association study of cutaneous basal cell carcinoma (BCC)1, we describe here several new susceptibility...Full Text Available

2009-08-01

Multiple Genes on Chromosome 7 Regulate Dopaminergic Amacrine Cell Number in the Mouse Retina

BackgroundIndividuals living in malaria endemic areas generally harbour multiple parasite strains. Multiplicity of infection (MOI) can be an indicator of immune status. However,...Full Text Available

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Investigation of Dyslexia and SLI Risk Variants in Reading- and Language-Impaired Subjects

PurposeThe size of neuronal populations is modulated by gene variants that influence cell production and survival, in turn influencing neuronal connectivity, function,...Full Text Available

2009-05-01

Identification of the binding domain for NADP"+ of human glucose-6-phosphate dehydrogenase by sequence analysis of mutants

Dyslexia (or reading disability) and specific language impairment (or SLI) are common childhood disorders that show considerable co-morbidity and diagnostic overlaps and have been suggested to share...Full Text Available

2011-01-01

Functional significance of UDP-glucuronosyltransferase (UGT) variants in the metabolism of active tamoxifen metabolites

Human erythrocyte glucose-6-phosphate is normally quite stable in the presence of 10 #mu#M NADP"+. Certain glucose-6-phosphate dehydrogenase variants lose virtually all their activity at this concentration of NADP"+ but are reactivated by 200 #mu#M NADP"+. Such variants presumably have a defect in their NADP"+-binding site. The authors analyzed the sequence of cDNA or genomic DNA from seven unrelated patients with hemolytic anemia due to the inheritance of variants that are reactivated by NADP"+. Six patients had substitutions of one of three adjacent amino acids, and the seventh patient had another amino acid substitution 23 residues downstream. These amino acids are highly conserved, all being present in rat and all but one being found also in Drosophila. The anomalous electrophoretic behavior of some of the variants can be explained by their loss of ability to bind NADP"+. The conclude that the ...

Endovascular treatment of a Superior Mesenteric Artery Syndrome variant secondary to traumatic pseudoaneurysm

Tamoxifen (TAM) is a selective estrogen receptor modulator widely used in the prevention and treatment of breast cancer. A major mode of metabolism of the major active metabolites of TAM, 4-OH-TAM...Full Text Available

2009-03-01

Detecting variants with Metabolic Design, a new software tool to design probes for explorative functional DNA microarray development

Pseudoaneurysms related to the superior mesenteric artery (SMA) are a recognised complication of trauma to the vessel, and successful treatment with stenting has been previously described. We report...Full Text Available

Dedifferentiated liposarcoma of the small bowel mesentery presenting as a submucosal mass

BackgroundMicroorganisms display vast diversity, and each one has its own set of genes, cell components and metabolic reactions. To assess their huge unexploited metabolic potential...Full Text Available

Common Genetic Variants Associate with Serum Phosphorus Concentration

Dedifferentiated liposarcoma (DDLPS) is a variant of liposarcoma but with a more aggressive course. It occurs most commonly in the retroperitoneum and rarely in any other anatomical location. We describe...Full Text Available

2011-07-15

Cellobiose dehydrogenase from Phanerochaete chrysosporium is encoded by two allelic variants.

Phosphorus is an essential mineral that maintains cellular energy and mineralizes the skeleton. Because complex actions of ion transporters and regulatory hormones regulate serum phosphorus concentrations,...Full Text Available

2010-07-01

Animal models of human amyloidoses: Are transgenic mice worth the time and trouble?

The complete nucleotide sequences of two alleles of cellobiose dehydrogenase, cdh-1 (3,627 bp) and cdh-2 (3,623 bp), from Phanerochaete chrysosporium OGC101 are reported. The nucleotide sequences of...Full Text Available

1997-02-01

Angiomatoid giant cellular blue nevus of vaginal wall associated with pregnancy

The amyloidoses are the prototype gain of toxic function protein misfolding diseases. As such, several naturally occurring animal models and their inducible variants provided some of the first...Full Text Available

2009-08-20

A Spindle Cell Predominant Pancreatic Solid-pseudopapillary Tumor

BackgroundBlue nevi that arise from the Müllerian tract are rare melanocytic lesions. Several histopathologic variants of cellular blue nevi have been described. The angiomatoid...Full Text Available

A Nitrate Reductase-less Variant Isolated from Suspension Cultures of Datura innoxia (Mill.) ¹

A hitherto unrecognized variant of solid-pseudopapillary tumor (SPT) of the pancreas is reported. The tumor presented in the pancreatic tail of a 44-year-old female patient. It was a well-defined, solid...Full Text Available

2008-08-30

A Cytogenetic Abnormality and Rare Coding Variants Identify ABCA13 as a Candidate Gene in Schizophrenia, Bipolar Disorder, and Depression

A comparative study has been carried out of the growth of two lines of Datura innoxia (Mill.) cells, designated DI-6 and NR1, their resistance to chlorate, and their ability to assimilate...Full Text Available

1980-10-01

Transferrin fails to provide protection against Fas-induced hepatic injury in mice with deletion of functional transferrin-receptor type 2

Schizophrenia and bipolar disorder are leading causes of morbidity across all populations, with heritability estimates of ∼80% indicating a substantial genetic component. Population genetics...Full Text Available

2009-12-11

Mutational Analysis of the Carboxy-Terminal (YGX)₄ Repeat Domain of CpsD, an Autophosphorylating Tyrosine Kinase Required for Capsule Biosynthesis in Streptococcus pneumoniae

We reported previously that Fas-induced hepatic failure in normal mice was attenuated or prevented by exogenous transferrin (Tf), particularly apoTf. Here we show in C57BL6J/129 mice with genetic...Full Text Available

2008-08-01

Mice With a Deletion in the Gene for CCAAT/Enhancer-Binding Protein ? are Protected Against Diet-Induced Obesity

In Streptococcus pneumoniae, CpsB, CpsC, and CpsD are essential for encapsulation, and mutants containing deletions of cpsB, cpsC, or cpsD...Full Text Available

2003-05-01

Metabolomic analysis of the plant pathogenic fungi Fusarium graminearum and Fusarium culmorum

The CCAAT/enhancer-binding protein β (C/EBPβ) is required for adipocyte differentiation and maturation. We have studied the role of the transcription factor, C/EBPβ,...Full Text Available

2007-01-01

Environmental Research Database

DescriptionThis project is part of the BBSRCs special initiative on plant and microbial metabolomics. The project will primarily focus on the trichothecene mycotoxin producing Ascomycete fungus Fusarium graminearum (Fg) which causes ear blight disease of small grain cereals. The project aims to explore the metabolome of various wild-type and single gene deletion Fg strains and to compare some of these with the identical gene mutation in the budding yeast, S. cerevisiae (Sc) and the saprophytic filamentous [continued...

2008-01-31

Interplay of Reverse Transcriptase Inhibitor Therapy and Gag p6 Diversity in HIV Type 1 Subtype G and CRF02_AG

Deletion of Genes Implicated in Protecting the Integrity of Male Germ Cells Has Differential Effects on the Incidence of DNA Breaks and Germ Cell Loss

AbstractThe gag p6 region of HIV-1 has various nonsubstitutionary mutations, including insertions, duplications, deletions, and premature stop codons. Studies have linked gag p6 mutations...Full Text Available

2008-09-01

Chimeric Matrix Proteins Encoded by Defective Proviruses with Large Internal Deletions in Human T-Cell Leukemia Virus Type 1-Infected Humans

BackgroundInfertility affects ∼20% of couples in Europe and in 50% of cases the problem lies with the male partner. The impact of damaged DNA originating...Full Text Available

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Mitochondrial DNA variants in Drosophila melanogaster are expressed at the level of the organismal phenotype.

Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent of adult T-cell leukemia/lymphoma (ATLL), HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), and other diseases....Full Text Available

2000-05-01

The ZNF804A gene: characterization of a novel neural risk mechanism for the major psychoses.

A plethora of experimental studies use mtDNA as a marker of demographic processes without questioning the possibility that selection may bias their interpretations. We studied four lines of Drosophila melanogaster that have a standardized nuclear DNA but variable mtDNA. We completed the sequencing of the mitochondrial genomes (excluding the A+T rich region) and compiled the differences. We then assayed male influence on oviposition, starvation resistance, lipid proportion and physical activity. We discuss these results in terms of the known differences between the lines and conclude that naturally occurring mtDNA variants in D. melanogaster are expressed at the level of the organismal phenotype. PMID:21757031

2011-07-05

PICTURE: an interactive tool for creating visual aids. Reference manual, version 2. 0

Schizophrenia and bipolar disorder share genetic risk, brain vulnerability, and clinical symptoms. The ZNF804A risk variant, rs1344706, confers susceptibility for both disorders. This study aimed to identify neural mechanisms common to both schizophrenia and bipolar disorder through this variant's potential effects on cortical thickness, white matter tract integrity, and cognitive function. Imaging, genetics, and cognitive measures were ascertained in 62 healthy adults aged between 18 and 59 years. High-resolution multimodal MRI/DTI imaging was used to measure cortical thickness and major frontotemporal and interhemispheric white matter tracts. The general linear model was used to examine the influence of the ZNF804A rs1344706 risk variant on cortical thickness, white matter tract integrity, and cognitive measures. Individuals homozygous for the risk variant ('A' allele) demonstrated reduced cortical ...

2011-04-27

Origin and spread of a common deletion causing mucolipidosis type II: insights from patterns of haplotypic diversity

The PICTURE utility generates user defined pictures with black-and-white or color output, suitable for viewgraphs, slides, report diagrams - in general, any application requiring visual aids. The interactive menu-driven system allows a user to create a picture consisting of text, straight lines, graphs, and assortment of markers, splines and drawings, all in a variety of colors. Commands for manipulating the picture include moving, scaling, rotating, deleting, copying and changing the color of objects. Text strings may be aligned left, right, or centered, and have a vertical or horizontal path. Markers, circles and the large bold font may be filled or unfilled. The user may add or delete one of two overlay grids for help in positioning items. The final product may be saved in a text file for later retrieval. PICTURE currently runs on a VAX 11/780 with the DI-3000 commercial graphics package. Supported graphics devices include Ramtek 9400 and ...

1984-01-20

British Library Electronic Table of Contents (United Kingdom)

Coutinho MF, Encarnao M, Gomes R, da Silva Santos L, Martins S, Sirois-Gagnon D, Bargal R, Filocamo M, Raas-Rothschild A, Tappino B, Laprise C, Cury GK, Schwartz IV, Artigals O, Prata MJ, Alves S. Origin and spread of a common deletion causing mucolipidosis type II: insights from patterns of haplotypic diversity. Mucolipidosis II (ML II alpha/beta), or I-cell disease, is a rare genetic disease in which activity of the uridine diphosphate (UDP)-N-acetylglucosamine:lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-phosphotransferase) is absent. GlcNAc-phosphotransferase is a multimeric enzyme encoded by two genes, GNPTAB and GNPTG. A spectrum of mutations in GNPTAB has been recently reported to cause ML II alpha/beta. Most of these mutations were found to be private or rare. ...

2011-01-01

Protocol for the E-Area Low Level Waste Facility Disposal Limits Database

Plasmid-conferred tetracycline resistance confers collateral cadmium sensitivity to E. coli cells

A database has been developed to contain the disposal limits for the E-Area Low Level Waste Facility (ELLWF). This database originates in the form of an EXCEL{copyright} workbook. The pertinent sheets are translated to PDF format using Adobe ACROBAT{copyright}. The PDF version of the database is accessible from the Solid Waste Division web page on SHRINE. In addition to containing the various disposal unit limits, the database also contains hyperlinks to the original references for all limits. It is anticipated that database will be revised each time there is an addition, deletion or revision of any of the ELLWF radionuclide disposal limits.

2006-01-31

Computer-assisted rotation and multiple stationary irradiation technique

E. coli HB101 cells transformed to tetracycline resistance with the plasmids pMB9 or pBR322 display a 10/sup 5/-10/sup 6/-fold lower plating efficiency on agar containing 440 ..mu..M CdCl/sub 2/ than nontransformed cells. When DNA is inserted into the BamH1 site of the plasmid tet gene, or when DNA spanning the BamH1 site is deleted, tetracycline resistance and cadmium hypersensitivity are both lost. In contrast, insertion of DNA into the ampicillin resistance gene does not affect cadmium hypersensitivity.

1982-01-01

Radiation-induced mutations at mouse hemoglobin loci

A computer-assisted rotation and stationary conformation radiotherapy system with overrunning multileaf collimators has been developed. This system can produce any shape of target volume, including a number of target areas outside the axis of the rotation, regardless of the location of the axis at 360"0 rotation irradiation. In addition, by changing the dose rate and deleting a partial region within a field, multiple stationary irradiation can produce more homogeneous target volumes - thus avoiding excessive irradiation to critical organs - than the rotation technique. (orig.).

Y Chromosome microdeletion and altered sperm quality in human males with high concentration of seminal hexachlorocyclohexane (HCH).

In experiments designed to detect new mutations affecting hemoglobin, we irradiated the male or female parent in reciprocal crosses of two mouse strains that differ in alleles at the hemoglobin (Hba, Hbb) loci as well as at five other specific loci. Offspring were analyzed for hemoglobin properties (electrophoretic pattern, solubility, crystal pattern), serum albumin differences, erythrocyte lysis, reticulocyte count, and external appearance. Five hemoglobin variants were found among the mutants. In three, the genetic contribution from the irradiated father was not expressed with regard to the #alpha#-chain; one carried a tandem duplication (the first known case in the mouse) involving Hbb; and one probably resulted from double nondisjunction of chromosome 7. The finding that major chromosome aberratios can mimic hemoglobin mutations indicates the need, in similar experiments, to follow F_1 screening with thorough cytogenetic analysis. The ...

Three novel mutations responsible for Cockayne syndrome group A

Recent studies have shown Y chromosome microdeletions associated with male infertility. The factors responsible for Y chromosome microdeletions in spermatozoa remain unresolved. However, the environmental pollutants are known to damage DNA in differentiating and maturing germ cells in the male reproductive tract. Therefore, the aim of this study was to investigate the effects of seminal hexachlorocyclohexane (HCH) and its isomers, an environmental pollutant, in 50 fertile and 50 infertile males in relation to semen quality and the incidence of Y chromosome microdeletion in azoospermic factor (AZF) region. As compared to control, an increased HCH level and significantly decreased semen quality were observed in the infertile males. A positive significant association was found between sperm count with alpha-HCH and beta-HCH in the infertile males. A negative significant association was observed between sperm counts with gamma-HCH in asthenospermia patients and with beta-HCH and total HCH ...

2010-06-18

Mapping a nucleolar targeting sequence of an RNA binding nucleolar protein, Nop25

Cockayne syndrome (CS) is a rare autosomal recessive disease, which shows diverse clinical symptoms such as photosensitivity, severe mental retardation and developmental defects. CS cells are hypersensitive to killing by ultraviolet (UV)-irradiation and defective in transcription-coupled repair. Two genetic complementation groups in CS (CS-A and CS-B) have been identified. We analyzed mutations of the CSA gene in 5 CS-A patients and identified 3 types of mutations. Four unrelated CS-A patients (CS2OS, CS2AW, Nps2 and CS2SE) had a deletion including exon 4, suggesting that there is a founder effect on the CSA mutation in Japanese CS-A patients. Patient CS2SE was a compound heterozygote for this deletion and an amino acid substitution at the 106th glutamine to proline (Q106P) in the WD-40 repeat motif of the CSA protein, which resulted in a defective nucleotide excision repair. Patient Mps1 had a large deletion in the ...

2003-02-01

A genetic model of melanoma tumorigenesis based on allelic losses

Nop25 is a putative RNA binding nucleolar protein associated with rRNA transcription. The present study was undertaken to determine the mechanism of Nop25 localization in the nucleolus. Deletion experiments of Nop25 amino acid sequence showed Nop25 to contain a nuclear targeting sequence in the N-terminal and a nucleolar targeting sequence in the C-terminal. By expressing derivative peptides from the C-terminal as GFP-fusion proteins in the cells, a lysine and arginine residue-enriched peptide (KRKHPRRAQDSTKKPPSATRTSKTQRRRR) allowed a GFP-fusion protein to be transported and fully retained in the nucleolus. When the peptide was fused with cMyc epitope and expressed in the cells, a cMyc epitope was then detected in the nucleolus. Nop25 did not localize in the nucleolus by deletion of the peptide from Nop25. Furthermore, deletion of a subdomain (KRKHPRRAQ) in the peptide or amino acid substitution of lysine and arginine ...

2006-06-10

Texture transformations in thermomechanically treated steels having increased nitrogen content and alloys

Previous karyotypic studies have indicated a possible series of non-random chromosomal events involved in the progression of melanoma. We sought to define a model of melanocyte tumorigenesis by studying allelic deletions of polymorphic simple tandem repeat markers mapping to chromosome 1, 6q, 7, 9p, 10, 11, 17, and 21 in thirty matched pairs of melanoma and constitutional DNAs. The most frequent and earliest deletions were found on 9p (57%) and 10q (32%) and with the exception of one case, no sample has loss of markers on another chromosome without concomitant loss of markers on 9p and/or 10q. Losses on 6q were also a frequent (32%) event that sometimes occurred in primary melanomas, whereas losses of loci on distal 1p (26%) or 11q (26%) occurred only in metastic melanomas. A background rate (0-17%) of allele loss was seen on chromosomes 7, 17, and 21. Homozygous deletions in a panel of 31 melanoma cell lines were only ...

1994-09-01

Strategies and computational tools for improving randomized protein libraries.

In this study the results of mathematical simulation and experimental research of texture transition in stress assisted shear phase transformations are discussed. The orientation distribution function (ODF) calculation model to the any type crystalline lattice symmetry materials has been suggested. By crystalline lattice symmetry analysis this computer program determines necessary amount of the initial experimental data. The experimental verification supports this model well. The ODF calculation model after shear phase transformation with equal use of all possible variants of orientation relationship or with various degrees of variant selection worked out. In this study the experimental and calculating data of the direct and reversal shear transformations #alpha# <=> #gamma# in Fe-alloys and B2 <=> B19' in Ti-Ni alloys are discussed. The stress assisted shear phase transformation model and computer program to calculate ODF after ...

Quantization in the presence of Gribov ambiguities

In the last decade, directed evolution has become a routine approach for engineering proteins with novel or altered properties. Concurrently, a trend away from purely 'blind' randomization strategies and towards more 'semi-rational' approaches has also become apparent. In this review, we discuss ways in which structural information and predictive computational tools are playing an increasingly important role in guiding the design of randomized libraries: web servers such as ConSurf-HSSP and SCHEMA allow the prediction of sites to target for producing functional variants, while algorithms such as GLUE, PEDEL and DRIVeR are useful for estimating library completeness and diversity. In addition, we review recent methodological developments that facilitate the construction of unbiased libraries, which are inherently more diverse than biased libraries and therefore more likely to yield improved variants. PMID:16095966

2005-10-01

Ecological utilization of wastes. A manual for optimum waste management concepts. 3. rev. ed. Oekologische Muellverwertung. Handbuch fuer optimale Abfall-Konzepte

The non-perturbative validity of covariant BRST-quantization of gauge theories on compact Euclidean space-time manifolds is reviewed. BRST-quantization is related to the construction of a Topological Quantum Field Theory (TQFT) of Witten type on the gauge group. The criterion for the non-perturbative validity of the quantization is that the partition function of the corresponding TQFT does not vanish and that its (equi-variant) BRST-algebra is free of anomalies. I sketch the construction of a TQFT whose partition function is proportional to the generalized Euler-characteristic of the coset space S U (n){sub gauge} / SU(n){sub global} with an associated equi-variant BRST-algebra that manifestly preserves translational symmetry. Some non-perturbative consequences of this approach are discussed. (author)

1999-07-01

The normal developmental regulation of a cloned sgs3 'glue' gene chromosomally integrated in Drosophila melanogaster by P element transformation.

From out of the numerous technical alternatives the manual develops a waste management concept with emphasis on methods which can be coordinated for maximum ecological use and minimum economic costs. A clear-cut analysis of the present state of waste utilization and waste disposal in the Federal Republic of Germany (chapter 1) is followed by a detailed description of conventional and modern state-of-the-art waste utilization methods based on ecological evaluation criteria (chapter 3). An optimum waste utilization concept for defined quantities of wastes is derived from the ecological and economic comparison of waste utilization techniques given in chapter 3. Chapter 4 points out different variants of waste utilization and waste disposal methods and shows how to determine the optimum variant of the optimum concept for a particular area or community. (orig.).

1991-01-01

The human gastrin/cholecystokinin type B receptor gene: alternative splice donor site in exon 4 generates two variant mRNAs.

A 7-kb genomic segment containing the coding sequence for the Drosophila melanogaster Formosa variant of salivary gland secretion protein 3 (sgs3) has been inserted into the snw y, bw, st strain of...Full Text Available

1983-01-01

The PTPN22 allele encoding an R620W variant interferes with the removal of developing autoreactive B cells in humans

Gastrin and its carboxyl-terminal homolog cholecystokinin (CCK) exert a variety of biological actions in the brain and gastrointestinal tract that are mediated in part through one or more G protein-coupled...Full Text Available

1993-10-01

100

Ternary aluminides LnPd[sub 2]Al with YPd[sub 2]Si-type structure

Protein tyrosine phosphatase nonreceptor type 22 (PTPN22) gene polymorphisms are associated with many autoimmune diseases. The major risk allele encodes an R620W amino acid change that...Full Text Available

2011-09-01

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101

Selective Expression in Carotid Body Type I Cells of a Single Splice Variant of the Large Conductance Calcium- and Voltage-activated Potassium Channel Confers Regulation by AMP-activated Protein Kinase*

Attempts to synthesize YPd[sub 2]Si-type representatives LnPd[sub 2]Al were successful only with Ln triple bond Ce, Pr, Nd. This orthorhombic structure (oP16; space group Pnma) is an ordered variant of the Fe[sub 3]C type. Only NdPd[sub 2]Al undergoes magnetic ordering above 2 K. (orig.)

1993-04-13

102

Prediction of conformational changes by single mutation in the hepatitis B virus surface antigen (HBsAg) identified in HBsAg-negative blood donors

Inhibition of large conductance calcium-activated potassium (BK_Ca) channels mediates, in part, oxygen sensing by carotid body type I cells. However, BK_Ca channels remain active...Full Text Available

2011-04-08

103

Polyadenylated H3 histone transcripts and H3 histone variants in alfalfa.

BackgroundSelection of hepatitis B virus (HBV) by host immunity has been suggested to give rise to variants with amino acid substitutions at or around the 'a' determinant...Full Text Available

104

Pitting corrosion on a copper canister; Gropfraetning paa kopparkapsel

Histone H3 mRNAs were found in polyA(+) fractions of total RNA prepared from alfalfa plants, calli and somatic embryos. The sequence analysis of cDNAs revealed the presence of a polyA tail on independent...Full Text Available

1989-04-25

105

High yielding mutants in sesamum

It is demonstrated that normal pitting can occur during oxidizing conditions in the repository. It is also concluded that a new theory for pitting corrosion has to be developed, as the present theory is not in accordance with all practical and experimental observations. A special variant of pitting, based on the growth of sulfide whiskers, is suggested to occur during reducing conditions. However, such a mechanism needs to be demonstrated experimentally. A simple calculational model of canister corrosion was developed based on the results of this study. 69 refs, 3 figs.

1996-02-01

106

Helper T-Cell Epitopes Encoded by the Babesia bigemina rap-1 Gene Family in the Constant and Variant Domains Are Conserved among Parasite Strains

Mutation breeding in one of the cultigens TMV-5 in Sesamum is undertaken with 5 kr, 10 kr, 15 kr, 20 kr, 60 kr and 80 kr, dosages of gamma-rays. 60 kr and 80 kr dosages were found to be lethal from the complete failure of seed germination. The mutagen had a heterotic effect on the different characters and the promising variants obtained in this programme are proposed to be maintained by pureline breeding for their possible release as new varieties. (author).

107

Heat recovery in foundries

Among important candidates for babesial vaccines are apical complex proteins, including rhoptry-associated protein 1 (RAP-1) from Babesia bovis and B. bigemina, which...Full Text Available

1998-04-01

108

Enantioselective Transformation of ?-Hexachlorocyclohexane by the Dehydrochlorinases LinA1 and LinA2 from the Soil Bacterium Sphingomonas paucimobilis B90A

A great deal of energy is necessary to manufacture castings, the greater part of which is wasted. Some general instructions are given on how to keep this part as low as possible. This is discussed particularly using the example of a heat recovery plant for foundries, especially in the melting and cooling of castings with the use of cupola furnace stack gas to produce hot gas. Further variants for recovering heat from the individual stages of a foundry process are listed. There are data on using this heat in operation.

1982-01-01

109

Chromosomal Integration of the Extended-Spectrum ?-Lactamase Gene bla_CTX-M-15 in Salmonella enterica Serotype Concord Isolates from Internationally Adopted Children?

Sphingomonas paucimobilis B90A contains two variants, LinA1 and LinA2, of a dehydrochlorinase that catalyzes the first and second steps in the metabolism of hexachlorocyclohexanes (R....Full Text Available

2005-12-01

110

Astrocytes derived from trisomic human embryonic stem cells express markers of astrocytic cancer cells and premalignant stem-like progenitors

We report the emergence of Salmonella enterica isolates of serotype Concord (and its monophasic variant 6,7:l,v:-) producing the extended-spectrum β-lactamases (ESBLs) SHV-12...Full Text Available

2009-05-01

111

A quenched c = 1 critical matrix model

BackgroundTrisomic variants of human embryonic stem cells (hESCs) arise spontaneously in culture. Although trisomic hESCs share many properties with diploid hESCs, they also exhibit...Full Text Available

112

A genome-wide association study of cleft lip with and without cleft palate identifies risk variants near MAFB and ABCA4

We study a variant of the Penner-Distler-Vafa model, proposed as a c = 1 quantum gravity: quenched' matrix model with logarithmic potential. The model is exactly soluble, and exhibits a two-cut branching as observed in multicritical unitary matrix models and multicut Hermitian matrix models. Using analytic continuation of the power in the conventional polynomial potential, we also show that both the Penner-Distler-Vafa model and our quenched' matrix model satisfy Virasoro algebra constraints.

1990-12-01

113

Identification and characterization of noncoding small RNAs in Streptococcus pneumoniae serotype 2 strain D39.

Case-parent trios were used in a genome wide association study of cleft lip with/without cleft palate (CL/P). SNPs near two genes not previously associated with CL/P [MAFB:...Full Text Available

2010-06-01

114

Global Molecular Characterization of the Chromate Stress Response in Shewanella oneidensis MR-1: Identification of a Putative DNA-Binding Response Regulator and Azoreductase Involved in Cr(VI) Detoxification

We report a search for small RNAs (sRNAs) in the low-GC, gram-positive human pathogen Streptococcus pneumoniae. Based on bioinformatic analyses by Livny et al. (J. Livny, A. Brencic, S. Lory, and M. K. Waldor, Nucleic Acids Res. 34:3484-3493, 2006), we tested 40 candidates by Northern blotting and confirmed the expression of nine new and one previously reported (CcnA) sRNAs in strain D39. CcnA is one of five redundant sRNAs reported by Halfmann et al. (A. Halfmann, M. Kovacs, R. Hakenbeck, and R. Bruckner, Mol. Microbiol. 66:110-126, 2007) that are positively controlled by the CiaR response regulator. We characterized 3 of these 14 sRNAs: Spd-sr17 (144 nucleotides [nt]; decreased in stationary phase), Spd-sr37 (80 nt; strongly expressed in all growth phases), and CcnA (93 nt; induced by competence stimulatory peptide). Spd-sr17 and CcnA likely fold into structures containing single-stranded regions between hairpin structures, whereas Spd-sr37 forms a base-paired structure. Primer ...

2010-01-01

115

Epitope mapping and functional analysis of sigma A and sigma NS proteins of avian reovirus

Shewanella oneidensis MR-1 is a model environmental organism that possesses diverse respiratory capacities, including the ability to reduce soluble Cr(VI) to sparingly soluble, less toxic Cr(III). Effective bioremediation of Cr-contaminated sites requires knowledge of the molecular mechanisms and regulation of heavy metal resistance and biotransformation by dissimilatory metal-reducing bacteria. Towards this goal, our ERSP-funded work is focused on the identification and functional analysis of genes/proteins comprising the response pathways for chromate detoxification and/or reduction. Previous transcriptomic profiling and whole-cell proteomic analyses implicated the involvement of a functionally undefined DNA-binding response regulator (SO2426) and a putative azoreductase (SO3585) in the chromate stress response of MR-1. Here we describe a detailed functional analysis of SO2426 and SO3585 in order to begin to understand the role of these proteins in the cellular response to chromate. ...

2006-04-05

116

The type III secretion system is involved in Escherichia coli K1 interactions with Acanthamoeba

We have previously shown that avian reovirus (ARV) #sigma#A and #sigma#NS proteins possess dsRNA and ssRNA binding activity and suggested that there are two epitopes on #sigma#A (I and II) and three epitopes (A, B, and C) on #sigma#NS. To further define the location of epitopes on #sigma#A and #sigma#NS proteins and to further elucidate the biological functions of these epitopes by using monoclonal antibodies (MAbs) 62, 1F9, H1E1, and 4A123 against the ARV S1133 strain, the full-length and deletion fragments of S2 and S4 genes of ARV generated by polymerase chain reaction (PCR) were cloned into pET32 expression vectors and the fusion proteins were overexpressed in Escherichia coli BL21 strain. Epitope mapping using MAbs and E. coli-expressed deletion fragments of #sigma#A and #sigma#NS of the ARV S1133 strain, synthetic peptides, and the cross reactivity of MAbs to heterologous ARV strains demonstrated that epitope II on #sigma#A was located at ...

2005-02-20

117

British Library Electronic Table of Contents (United Kingdom)

The type III secretion system among Gram-negative bacteria is known to deliver effectors into host cell to interfere with host cellular processes. The type III secretion system in Yersina, Pseudomonas and Enterohemorrhagic Escherichia coli have been well documented to be involved in the bacterial pathogenicity. The existence of type III secretion system has been demonstrated in neuropathogenic E. coli K1 strains. Here, it is observed that the deletion mutant of type III secretion system in E. coli strain EC10 exhibited defects in the invasion and intracellular survival in Acanthamoeba castellanii (a keratitis isolate) compared to its parent strain. Next, it was determined whether type III secretion system plays a role in E. coli K1 survival inside Acanthamoeba during the encystment process...

2011-01-01

118

The propagation of relativistic heavy ions in multielement beam lines.

The Shi arrangement and the Ish arrangement

We describe calculations of the energy loss, range, stopping power, multiple scattering, and other related properties of a high-energy heavy-ion beam at any one of a set of beam line elements. A beam line element (e.g., any beam modification, detection, or control device) is characterized by its thickness, areal density, aperture, and function. The loss of multiply scattered particles to any finite-aperture detector is calculated in the small-angle approximation, and the position of the Bragg peak, as given by particles stopping in the second of two ionization chambers used for Bragg curve measurements, is estimated. A general purpose computer program, PROPAGATE, has been written to allow addition, deletion, and modification of the beam line elements used in the calculation and to provide a convenient means of repeating such calculations for arbitrary beam lines. Calculations and experimental measurements are compared and found to be in satisfactory agreement. ...

119

Montana Rivers Information System : Edit/Entry Program User's Manual.

This paper is about two arrangements of hyperplanes. The first --- the Shi arrangement --- was introduced by Jian-Yi Shi to describe the Kazhdan-Lusztig cells in the affine Weyl group of type $A$. The second --- the Ish arrangement --- was recently defined by the first author who used the two arrangements together to give a new interpretation of the $q,t$-Catalan numbers of Garsia and Haiman. In the present paper we will define a mysterious "combinatorial symmetry" between the two arrangements and show that this symmetry preserves a great deal of information. For example, the Shi and Ish arrangements share the same characteristic polynomial, the same numbers of regions, bounded regions, dominant regions, regions with $c$ "ceilings" and $d$ "degrees of freedom", etc. Moreover, all of these results hold in the greater generality of "deleted" Shi and Ish arrangements corresponding to an arbitrary subgraph of the complete graph. Our proofs are based on nice ...

2010-01-01

120

Involvement of a putative response regulator Brrg-1 in the regulation of sporulation, sensitivity to fungicides, and osmotic stress in Botrytis cinerea

The Montana Rivers Information System (MRIS) was initiated to assess the state`s fish, wildlife, and recreation value; and natural cultural, and geologic features. The MRIS is now a set of data bases containing part of the information in the Natural Heritage Program natural features and threatened and endangered species data bases and comprises of the Montana Interagency Stream Fisheries Database; the MDFWP Recreation Database; and the MDFWP Wildlife Geographic Information System. The purpose of this User`s Manual is to describe to the user how to maintain the MRIS database of their choice by updating, changing, deleting, and adding records using the edit/entry programs; and to provide to the user all information and instructions necessary to complete data entry into the MRIS databases.

1992-07-01

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121

British Library Electronic Table of Contents (United Kingdom)

The response regulator protein is a core element of two-component signaling pathway. In this study, we investigated functions of BRRG-1 of Botrytis cinerea, a gene that encodes a putative response regulator protein, which is homologous to Rrg-1 in Neurospora crassa. The BRRG-1 gene deletion mutant ?Brrg1-62 was unable to produce conidia. The mutant showed increased sensitivity to osmotic stress mediated by NaCl and KCl, and to oxidative stress generated by H2O2. Additionally, the mutant was more sensitive to the fungicides iprodione, fludioxonil, and triadimefon than the parental strain. Western-blot analysis showed that the Bos-2 protein, the putative downstream component of Brrg-1, was not phosphorylated in the ?Brrg1-62. Real-time polymerase chain reaction assays showed that expression ...

2011-01-01

122

Identification of mutations leading to the Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA

Hypoxia decreases sclerostin expression and increases Wnt signaling in osteoblasts

The Lesch-Nyhan (LN) syndrome is a severe X chromosome-linked disease that results from a deficiency of the purine salvage enzyme hypoxanthine phosphoribosyltransferase (HPRT). The mutations leading to the disease are heterogeneous and frequently arise as de novo events. The authors have identified nucleotide alterations in 15 independently arising HPRT-deficiency cases by direct DNA sequencing of in vitro amplified HPRT cDNA. They also demonstrate that the direct DNA sequence analysis can be automated, further simplifying the detection of new mutations at this locus. The mutations include DNA base substitutions, small DNA deletions, a single DNA base insertion, and errors in RNA splicing. The application of these procedures allows DNA diagnosis and carrier identification by the direct detection of the mutant alleles within individual families affected by LN.

1989-03-01

123

British Library Electronic Table of Contents (United Kingdom)

Mutations in sclerostin function or expression cause sclerosing bone dysplasias, involving decreased antagonism of Wnt/Lrp5 signaling. Conversely, deletion of the VHL tumor suppressor in osteoblasts, which stabilize HIF-a isoforms and thereby enables HIF-a/b-driven gene transcription, increases bone mineral content and cross-sectional area compared to wild-type controls. We examined the influence of cellular hypoxia (1% oxygen) upon sclerostin expression and canonical Wnt signaling. Osteoblasts and osteocytes cultured under hypoxia revealed decreased sclerostin transcript and protein, and increased expression and nuclear localization of activated b-catenin. Similarly, both hypoxia and the hypoxia mimetic DFO increased b-catenin gene reporter activity. Hypoxia and its mimetics increased exp...

2010-01-01

124

Functional domain analysis of glass, a zinc-finger-containing transcription factor in Drosophila.

Chromosomal rearrangement segregating with adrenoleukodystrophy: A molecular analysis

The glass gene is required for proper photo-receptor differentiation during development of the Drosophila eye glass codes for a DNA-binding protein containing five zinc fingers that we show is a transcriptional activator. A comparison of the sequences of the glass genes from two species of Drosophila and a detailed functional domain analysis of the Drosophila melanogaster glass gene reveal that both the DNA-binding domain and the transcriptional-activation domain are highly conserved between the two species. Analysis of the DNA-binding domain of glass indicates that the three carboxyl-terminal zinc fingers alone are necessary and sufficient for DNA binding. We also show that a deletion mutant of glass containing only the DNA-binding domain can behave in a dominant-negative manner both in vivo and in a cell culture assay that measures transcriptional activation. PMID:7604032

1995-07-01

125

Chromosomal localization and structure of the human type II IMP dehydrogenase gene

The relationship between X chromosome-linked adrenoleukodystrophy and the red/green color pigment gene cluster on Xq28 was investigated in a large kindred. The DNA in a hemizygous male showed altered restriction fragment sizes compatible with at least a deletion extending from the 5[prime] end of the color pigment genes. Segregation analysis using a DNA probe within the color pigment gene cluster showed significant linkage with adrenoleukodystrophy (logarithm of odds score of 3.19 at [theta] = 0.0). These data demonstrate linkage, rather than association, between a unique molecular rearrangement in the color pigment gene cluster and adrenoleukodystrophy. The DNA changes in this region are thus likely to be helpful for determining the location and identity of the responsible gene. 33 refs., 4 figs.

1993-10-15

126

A PAC containing the human mitochondrial DNA polymerase gamma gene (POLG) maps to chromosome 15q25

We determined the chromosomal localization and structure of the gene encoding human type II inosine 5{prime}-monophosphate dehydrogenase (IMPDH, EC 1.1.1.205), an enzyme associated with cellular proliferation, malignant transformation, and differentiation. Using polymerase chain reaction (PCR) primers specific for type II IMPDH, we screened a panel of human-Chinese hamster cell somatic hybrids and a separate deletion panel of chromosome 3 hybrids and localized the gene to 3p21.1{yields}p24.2. Two overlapping yeast artificial chromosome clones containing the full gene for type II IMPDH were isolated and a physical map of 117 kb of human genomic DNA in this region of chromosome 3 was constructed. The gene for type II IMPDH was localized and oriented on this map and found to span no more than 12.5 kb.

1994-05-01

127

Polymorphism in HTR3D shows different risks for acute chemotherapy-induced vomiting after anthracycline chemotherapy

The human mitochondrial DNA (mtDNA) is a closed circular, 16,569-bp double-stranded DNA, encoding 13 genes whose protein products are subunits of the oxidative phosphorylation system required for synthesis of most of the ATP consumed by eukaryotic cells. Point mutations of the mtDNA that cause multi-tissue, loss-of-energy syndromes, called mitochondrial encephalomyopathies (e.g., MERRF and MELAS), have been identified. In addition, large-scale deletions of the human mtDNA have been identified and are the molecular bases for the neonatal and adolescent onset loss-of-energy syndromes Pearson and Kearns-Sayer, respectively. 5 refs., 1 fig.

1997-03-01

128

British Library Electronic Table of Contents (United Kingdom)

Aims: Serotonin (5-hydroxytryptamine 3; 5-HT3) receptors are involved in chemotherapy-induced nausea and vomiting (CINV), and 5-HT3 antagonists are part of the `gold standard' antiemetic treatment during chemotherapy. We investigated the correlation of common variants in 5-HT3 receptor subunit genes with the occurrence of CINV. Materials & methods: A total of 110 previously characterized chemotherapy-naive women with primary breast cancer treated with anthracycline-containing chemotherapy served as a study group for mutational analysis by direct sequencing. Eight common SNPs in the 5-HT3 receptor genes, HTR3A, HTR3B, HTR3D and HTR3E, were selected for association analysis. Results: A nonsynonymous variant in HTR3D, p.G36A (rs6443930), was found to be over-represented in nonresponders, assu...

2010-01-01

129

Improvement of banana through biotechnology and mutation breeding

A CD33-specific single-chain immunotoxin mediates potent apoptosis of cultured human myeloid leukaemia cells.

Protocols were standardized for in vitro propagation of several elite and diverse banana accessions using shoot tip explants. Tissue culture raised plants were field planted at multiple locations. Studies were undertaken for the induction of mutations using multiple shoot cultures of six selected cultivars, Shreemanti (AAA), Basrai (AAA), Lal Kela (AAA), Rasthali (AAB), Karibale Monthan (ABB) and a wild diploid (BB). These shoot cultures were irradiated at different doses of gamma rays (0-100 Gy) and subcultured thrice (up to M_1V_3) to separate shimeras, followed by induction of rooting (M_1V_4). In general, the rate of multiplication had a negative association with the dose of gamma rays. Enhanced multiplication of shoots was noticed at lower doses. The proliferation of shoots was arrested beyond 50 Gy and a dose of 70 Gy was completely lethal for all the genotypes studied. The rooted plantlets were hardened in the green house and in the early stages of field growth, a few ...

1998-10-01

130

Unraveling photosystems. Final technical report

A novel single-chain immunotoxin was constructed by combining a CD33-specific single chain Fv (scFv) antibody fragment with an engineered variant of Pseudomonas exotoxin A (ETA). The variant toxin carries the KDEL peptide at its C-terminus, a cellular peptide mediating improved retrograde transport to the endoplasmic reticulum. The purified recombinant fusion protein induced potent apoptosis of the human myeloid cell lines U937, HL-60 and THP-1. Up to 98% of U937 cells were eliminated after treatment for 72 h with a single dose of 500 ng/ml (c. 7 nmol/l). Killing was antigen-specific and occurred by apoptosis. A control protein, consisting of a CD19-specific scFv antibody fragment fused to the ETA-KDEL toxin, failed to induce death of the CD19-negative cell lines U937, HL-60 and THP-1. The CD33-ETA toxin also mediated apoptosis of fresh patient-derived acute myeloid leukaemia cells from bone marrow and peripheral blood. The pronounced ...

2006-04-01

131

The p75"N"T"R tumor suppressor induces cell cycle arrest facilitating caspase mediated apoptosis in prostate tumor cells

This report highlights four main points. (1) A residue substitution in phosphoribulokinase of Synechocystis PCC 6803 renders the mutant light-sensitive. The authors isolated a light-sensitive mutant (BRLS) of the photosynthetic cyanobacterium Synechocystis 6803 that does not survive exposure to bright light; 70% of BRLS cells die upon exposure to light of > 3000 lux for 2 hr. (2) Excitation energy transfer from phycocyanin to chlorophyll in an apcA-defective mutant of Synechocystis sp. PCC 6803. A greenish mutant of the normally bule-green cyanobacterium Synechocystis sp. PC 6803, designated UV6p, was isolated and characterized. UV6p possesses functional photosystems I and II but lacks normal light harvesting phycobilisomes because allophycocyanin is absent and core-specific linker proteins are almost entirely absent. (3) Deletion of the psbG1 gene of the cyanobacterium Synechocystis sp. PCC 6803 leads to the activation of the cryptic psbG2 gene. The genes psbG1 ...

1997-09-01

132

Linkage analysis in familial Angelman syndrome

The p75 neurotrophin receptor (p75"N"T"R) is a death receptor which belongs to the tumor necrosis factor receptor super-family of membrane proteins. This study shows that p75"N"T"R retarded cell cycle progression by induced accumulation of cells in G0/G1 and a reduction in the S phase of the cell cycle. The rescue of tumor cells from cell cycle progression by a death domain deleted (#DELTA#DD) dominant-negative antagonist of p75"N"T"R showed that the death domain transduced anti-proliferative activity in a ligand-independent manner. Conversely, addition of NGF ligand rescued retardation of cell cycle progression with commensurate changes in components of the cyclin/cdk holoenzyme complex. In the absence of ligand, p75"N"T"R-dependent cell cycle arrest facilitated an increase in apoptotic nuclear fragmentation of the prostate cancer cells. Apoptosis of p75"N"T"R expressing cells occurred via the intrinsic mitochondrial pathway leading to a sequential caspase-9 and ...

2006-03-24

133

Performance Comparisons of PSO based Clustering

Familial Angelman syndrome (AS) can result from mutations in chromosome 15q11q13 that, when transmitted from father to child, result in no phenotypic abnormality but, when transmitted from mother to child, cause AS. These mutations therefore behave neither as dominant nor as recessive mutations but, rather, show an imprinted mode of inheritance. The authors have analyzed two sibling pairs with AS and a larger family with four AS offspring of three sisters with several recently described microsatellite polymorphisms in the AS region. AS siblings inherited the same maternal alleles at the GABRB3 and GABRA5 loci, and the unaffected siblings of AS individuals inherited the other maternal alleles at these loci. In one of the AS sibling pairs, analysis of a recombination event indicates that the mutation responsible for AS is distal to locus D15S63. This result is consistent with a previously described imprinted submicroscopic deletion causing AS, a ...

1993-07-01

134

IDEAS: The Science, Sociology and Economics of Food Production and Access to Food, Springer

In this paper we have investigated the performance of PSO Particle Swarm Optimization based clustering on few real world data sets and one artificial data set. The performances are measured by two metric namely quantization error and inter-cluster distance. The K means clustering algorithm is first implemented for all data sets, the results of which form the basis of comparison of PSO based approaches. We have explored different variants of PSO such as gbest, lbest ring, lbest vonneumann and Hybrid PSO for comparison purposes. The results reveal that PSO based clustering algorithms perform better compared to K means in all data sets.

2010-01-01

135

Wastenet

... (restricted)] 251-260 Revisiting new variant famine: the case of Swaziland by Scott Naysmith & Alex Waal & Alan Whiteside [Downloadable! (restricted)] 261-269 Food prices and the HIV response: findings from rapid regional assessments in eastern and southern Africa in 2008[InlineMediaObject not available: see fulltext.][InlineMediaObject not available: see fulltext.] by Stuart Gillespie & Paul Jere & John Msuya & Scott Drimie [Downloadable! (restricted)] 271-289 Declining global per capita agricultural production and warming oceans ...

136

Hypersersensitivity and Kounis syndrome due to a viper bite

British Library Electronic Table of Contents (United Kingdom)

A 60-year-old male was bitten by a venomous snake (Vipera ammodytes) and gradually developed signs of an allergic reaction including generalized itching, generalized rash, and chest discomfort. This was followed by severe retrosternal pain with electrocardiographic evidence of an inferior myocardial ischemia progressing to acute myocardial infarction. Cardiac enzymes and troponin, serum tryptase, and histamine were elevated. Coronary arteriography showed normal coronary arteries. This is a characteristic type I variant of Kounis syndrome, which is the concurrence of acute coronary syndromes with conditions associated with mast cell activation including allergic or hypersensitivity reactions as well as anaphylactic or anaphylactoid reactions. This is the first report to show that viper bite...

2006-01-01

137

Generalized Tonnetze

British Library Electronic Table of Contents (United Kingdom)

We study a generalization of the classical Riemannian Tonnetz to N-tone equally tempered scales (for all N) and arbitrary triads. We classify all the spaces that result. The torus turns out to be the most common possibility, especially as N grows. Other spaces include 2-simplices, tetrahedra boundaries, and the harmonic strip (in both its cylinder and Mobius band variants). The final and most exotic space we find is something we call a 'circle of tetrahedra boundaries'. These are the Tonnetze for spaces of triads which contain a tritone. They are closely related to Peck's Klein bottle Tonnetz.

2011-01-01

138

GIT Constructions of Moduli Spaces of Stable Curves and Maps

Enzymatic synthesis of N- and O-linked glycopeptides

This largely expository paper first gives an introduction to Hilbert stability and its use in Gieseker's GIT construction of $\\overline{M}_g$. Then I review recent work in this area--variants for unpointed curves that arise in Hassett's log minimal model program, starting with Schubert's moduli space of pseudostable curves, and constructions for weighted pointed stable curves and for pointed stable maps due to Swinarski and to Baldwin and Swinarski respectively. The focus is on the steps at which new ideas are needed. Finally, I list open problems in the area, particularly some arising in the log minimal model program that seem inaccessible to current techniques.

2008-01-01

139

Cubature Kalman smoothers

This paper describes the study of kinetically controlled enzymatic coupling of N- and O-glycopeptide fragments using subtilisin BPN' and two of its variants developed for use in high concentrations of dimethylformamide and in aqueous solution, respectively. Glycosyl amino acids were exploited as the P[sub 1], P[sub 2], P[sub 3], P'[sub 1], P'[sub 2], or P'[sub 3] residue in the enzymatic coupling. Glycosyltransferase-mediated glycosylation of the glycopeptide fragments obtained prior to or after enzymatic peptide bond formation is demonstrated. 19 refs., 5 figs., 1 tab.

1993-07-14

140

British Library Electronic Table of Contents (United Kingdom)

The cubature Kalman filter (CKF) is a relatively new addition to derivative-free approximate Bayesian filters built under the Gaussian assumption. This paper extends the CKF theory to address nonlinear smoothing problems; the resulting state estimator is named the fixed-interval cubature Kalman smoother (FI-CKS). Moreover, the FI-CKS is reformulated to propagate the square-root error covariances. Although algebraically equivalent to the FI-CKS, the square-root variant ensures reliable implementation when committed to embedded systems with fixed precision or when the inference problem itself is ill-conditioned. Finally, to validate the formulation, the square-root FI-CKS is applied to track a ballistic target on reentry.

2011-01-01

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141

Correlation between tensile property and micro-hardness in reduced activation ferritic/martensitic steel irradiated at 573 K

Testing and modification of a simple flow turbine; Messtechnische Untersuchung und Modifikation einer einfachen Durchstroemturbine

Full text of publication follows: Radiation hardening and embrittlement due to high-energy neutron radiation around 623 K are the important issues on reduced-activation ferritic/martensitic (RAF/M) steels. It is expected that the improvement of radiation hardening might be one of effective ways to control the mechanical properties of RAF/M after irradiation. It has been reported that the weld joint has less hardening than the base metal from the tensile test results of TIG weldments irradiated in HFIR. This report indicated that radiation hardening can be reduced by the optimization of heat treatment condition for F82H. The purposes of this study are to establish the condition of heat treatment for minimum of radiation hardening in F82H steel using Neutron/Ion-irradiation and to examine a correlation between tensile property and micro-hardness before/after irradiation. The materials used in this study were F82H IEA heat and F82H heat treatment variants. Neutron ...

2007-12-10

142

Thymidylate synthase expression and genotype have no major impact on the clinical outcome of colorectal cancer patients treated with 5-fluorouracil

A study conducted at the IHS had the aim to obtain basic information on whether it is possible to build flow turbines following the free jet principle that are on a par with, or superior to, the conventional design, especially in terms of performance and the efficiency curve. Several inflow contours with a ventilated free jet and a conventional flow turbine were studied. The direct comparison of these variants revealed a slightly enhanced peak load efficiency and a very distinct superiority of the free jet contour during partial load. But the efficiency of all variants failed to attain the values claimed by commercial manufacturers. As compared to the initial contour, peak load efficiency was enhanced by 5 per cent. During partial load, the variants studied showed a distinct superiority of the free jet concept over the contour of conventional flow turbines. (orig.) [Deutsch] Am IHS wurde eine Untersuchung mit dem Ziel ...

1997-12-31

143

British Library Electronic Table of Contents (United Kingdom)

Background and objectives.Thymidylate synthase (TS) expression levels appear to be related to response to 5-fluorouracil-(5-FU)-based chemotherapy in colorectal cancer (CRC) patients. Three polymorphisms have been proposed as modulators of TS expression: a tandemly repeated sequence (2R/3R) in the 5? UTR, a SNP (G > C) within the 3R allele and a 6 bp deletion in the 3' UTR.To evaluate the influence of TS expression and polymorphisms on clinical outcome of 5-FU-treated patients we performed a comprehensive genetic analysis on 63 CRC patients.Methods.TS expression levels were analyzed in normal and tumor tissues. TS coding sequence and UTR polymorphisms were investigated on DNA from normal tissue. LOH analysis was performed to determine tumor genotype.Results.A difference in disease-free sur...

2011-01-01

144

Skip-webs: Efficient distributed data structures for multi-dimensional data sets

Regulation of human ribosomal RNA transcription.

We present a framework for designing efficient distributed data structures for multi-dimensional data. Our structures, which we call skip-webs, extend and improve previous randomized distributed data structures, including skipnets and skip graphs. Our framework applies to a general class of data querying scenarios, which include linear (one-dimensional) data, such as sorted sets, as well as multi-dimensional data, such as d-dimensional octrees and digital tries of character strings defined over a fixed alphabet. We show how to perform a query over such a set of n items spread among n hosts using O(log n / log log n) messages for one-dimensional data, or O(log n) messages for fixed-dimensional data, while using only O(log n) space per host. We also show how to make such structures dynamic so as to allow for insertions and deletions in O(log n) messages for quadtrees, octrees, and digital tries, and O(log n / log log n) messages for one-dimensional data. Finally, we ...

2005-01-01

145

Peru-15, an improved live attenuated oral vaccine candidate for Vibrio cholerae O1.

We have used a cell-free polymerase I transcription system derived from HeLa cells to study the regulation of human rRNA synthesis. Analysis of deletion mutants spanning the start site of transcription at nucleotide +1 indicates that the control region affecting initiation of human rRNA synthesis is contained within sequences from nucleotides -158 to +18. This promoter region can be subdivided into (i) a central segment of approximately 40 base pair that is required for transcription and (ii) flanking sequences that influence the efficiency of transcription in vitro. We have examined the in vitro transcriptional activity of the human extract under various conditions that are thought to modulate rRNA synthesis in vivo. Cell-free extracts prepared from HeLa cells infected with adenovirus 2 synthesize human rRNA at levels greatly decreased relative to uninfected cell extracts. By contrast, in vitro transcription of human rRNA is stimulated 2- to 3-fold by the addition ...

1983-06-01

146

Nuclear Targeting of Methyl-Recycling Enzymes in Arabidopsis thaliana Is Mediated by Specific Protein Interactions.

Cholera vaccine candidate Peru-15 was derived from a Vibrio cholerae O1 El Tor Inaba strain by deleting the cholera toxin genetic element, introducing the gene encoding cholera toxin B subunit into recA, and screening for nonmotility. In a controlled study, Peru-15 (2 x 10(8) cfu) was administered to 11 volunteers. No vaccinee developed diarrhea, and 10 of 11 had > 4-fold rises in vibriocidal antibody titers. One month later, 5 vaccinees and 5 control volunteers were challenged with wild type V. cholerae O1. Four of 5 controls developed diarrhea (mean, 1.9 L). Two Peru-15 vaccinees developed diarrhea, 1 with < 0.3 L and 1 with approximately 1.0 L; this latter volunteer had not developed a significant vibriocidal immune response to vaccination. Peru-15 shows promise as a single-dose, oral cholera vaccine that is safe, immunogenic, and protective. PMID:7561195

1995-10-01

147

Modulating factors in the expression of radiation-induced oncogenic transformation

Numerous transmethylation reactions are required for normal plant growth and development. S-adenosylhomocysteine hydrolase (SAHH) and adenosine kinase (ADK) act coordinately to recycle the by-product of these reactions, S-adenosylhomocysteine (SAH) that would otherwise competitively inhibit methyltransferase (MT) activities. Here, we report on investigations to understand how the SAH produced in the nucleus is metabolized by SAHH and ADK. Localization analyses using green fluorescent fusion proteins demonstrated that both enzymes are capable of localizing to the cytoplasm and the nucleus, although no obvious nuclear localization signal was found in their sequences. Deletion analysis revealed that a 41-amino-acid segment of SAHH (Gly(150)-Lys(190)) is required for nuclear targeting of this enzyme. This segment is surface exposed, shows unique sequence conservation patterns in plant SAHHs, and possesses additional features of protein-protein interaction motifs. ADK ...

2011-10-01

148

Modulating factors in the expression of radiation-induced oncogenic transformation

Many assays for oncogenic transformation have been developed ranging from those in established rodent cell lines where morphological alteration is scored, to those in human cells growing in nude mice where tumor invasiveness is scored. In general, systems that are most quantitaive are also the least relevant in terms of human carcinogenesis and human risk estimation. The development of cell culture systems has made it possible to assess at the cellular level the oncogenic potential of a variety of chemical, physical and viral agents. Cell culture systems afford the opportunity to identify factors and conditions that may prevent or enhance cellular transformation by radiation and chemicals. Permissive and protective factors in radiation-induced transformation include thyroid hormone and the tumor promoter TPA that increase the transformation incidence for a given dose of radiation, and retinoids, selenium, vitamin E, and 5-aminobenzamide that inhibit the expression of transformation. ...

1990-08-01

149

Methodology for designing air-quality-monitoring networks. 1. Theoretical aspects

1990-01-01

150

Indirect boundary element method for three dimensional problems. Analytical solution for contribution to wave field by triangular element; Sanjigen kansetsu kyokai yosoho. Sankakukei yoso no kiyo no kaisekikai

An objective methodology is presented for determining the number and disposition of ambient air-quality stations in a monitoring network for the primary purpose of compliance with air-quality standards. The methodology utilizes a data base with real or simulated data from an air quality dispersion model for application with a two-step process for ascertaining the optimal monitoring network. In the first step, the air-quality patterns in the data base are collapsed into a single composite pattern through a figure-of-merit (FOM) concept. The most-desirable locations are ranked and identified using the resultant FOM fields. In the second step the network configuration is determined on the basis of the concept of spheres of influence (SOI) developed from cutoff values of spatial-correlation coefficients between potential monitoring sites and adjacent locations. The minimum number of required stations is then determined by deletion of lower-ranked stations whose ...

1986-01-01

151

Identification, characterization, and chromosomal localization of the human homolog (hES) of ES/130

Formulation is introduced for discretizing a boundary integral equation into an indirect boundary element method for the solution of 3-dimensional topographic problems. Yokoi and Takenaka propose an analytical solution-capable reference solution (solution for the half space elastic body with flat free surface) to problems of topographic response to seismic motion in a 2-dimensional in-plane field. That is to say, they propose a boundary integral equation capable of effectively suppressing the non-physical waves that emerge in the result of computation in the wake of the truncation of the discretized ground surface making use of the wave field in a semi-infinite elastic body with flat free surface. They apply the proposed boundary integral equation discretized into the indirect boundary element method to solve some examples, and succeed in proving its validity. In this report, the equation is expanded to deal with 3-dimensional topographic problems. A problem of a P-wave vertically ...

1997-05-27

152

Homo-d-lactic acid production from mixed sugars using xylose-assimilating operon-integrated Lactobacillus plantarum

The chicken extracellular matrix glycoprotein ES/130 is necessary for epithelial-mesenchymal transformation in the developing hear and is also expressed in noncardiac chicken tissues such as limb and notochord. We have identified hES, the human homology of chicken ES/130. Fluorescence in situ hybridization analysis (FISH) localizes hES to human chromosome 20p11.2-p12. FISH analyses of individuals with 20p12 deletions and affected by Alagille syndrome exclude hES as a candidate gene for this disorder. Reverse transcriptase-polymerase chain reaction studies reveal that hES is expressed in both fetal and adult human tissues and that hES expression in the left ventricle is increased in the failing adult heart. Further studies will evaluate how hES mutations may relate to congenital human cardiac and skeletal anomalies as well as cardiac remodeling in the adult. 16 refs., 2 figs.

1996-08-01

153

British Library Electronic Table of Contents (United Kingdom)

In order to achieve efficient d-lactic acid fermentation from a mixture of xylose and glucose, the xylose-assimilating xylAB operon from Lactobacillus pentosus (PXylAB) was introduced into an l-lactate dehydrogenase gene (ldhL1)-deficient Lactobacillus plantarum (?ldhL1-xpk1::tkt-?xpk2) strain in which the phosphoketolase 1 gene (xpk1) was replaced with the transketolase gene (tkt) from Lactococcus lactis, and the phosphoketolase 2 (xpk2) gene was deleted. Two copies of xylAB introduced into the genome significantly improved the xylose fermentation ability, raising it to the same level as that of ?ldhL1-xpk1::tkt-?xpk2 harboring a xylAB operon-expressing plasmid. Using the two-copy xylAB integrated strain, successful homo-d-lactic acid production was achieved from a mixture of 25?g/l xylos...

2011-01-01

154

Highly specific antiserum for arginine vasopressin

Ectopic expression of Cux1 is associated with reduced p27 expression and increased apoptosis during late stage cyst progression upon inactivation of Pkd1 in collecting ducts

A high sensitivity (titer 60-150,000) radioimmunoassay for arginine vasopressin (AVP) has been developed using synthetic AVP as antigen and standard. The specificity of the antiserum has been characterized in detail by measuring the relative binding affinities of 29 analogs and fragments of AVP. In general, single amino acid substitution or deletion in the AVP molecule caused a reduction in binding to the antiserum; binding was further impaired for analogs which had multiple substitutions. ''Ring'' and ''tail'' fragments of the neurohypophysial hormones did not bind to the antiserum, and the only analog which the antiserum could not differentiate from AVP was deamino-AVP. More specifically, the antiserum exhibited the following properties: position 1, no requirement for a position charge at "1/_2 Cys; positions 2 and 3, requirements for #pi#-#pi# interacting pair of aromatic side chains and for Tyr at position 2; position 4, requirement for Gln (>Thr > Val); ...

155

British Library Electronic Table of Contents (United Kingdom)

Abstract Polycystic kidney diseases (PKD) are inherited disorders characterized by fluid-filled cysts primarily in the kidneys. We previously reported differences between the expression of Cux1, p21, and p27 in the cpk and Pkd1 null mouse models of PKD. Embryonic lethality of Pkd1 null mice limits its study to early stages of kidney development. Therefore, we examined mice with a collecting duct specific deletion in the Pkd1 gene. Cux1 was ectopically expressed in the cyst lining epithelial cells of newborn, P7 and P15 Pkd1CD mice. Cux1 expression correlated with cell proliferation in early stages of cystogenesis, however, as the disease progressed, fewer cyst lining cells showed increased cell proliferation. Rather, Cux1 expression in late stage cystogenesis was associated with increased ...

2011-01-01

156

Crystal structure of Spot 14, a modulator of fatty acid synthesis

Absence of linkage of apparently single gene mediated ADHD with the human syntenic region of the mouse mutant coloboma

Spot 14 (S14) is a protein that is abundantly expressed in lipogenic tissues and is regulated in a manner similar to other enzymes involved in fatty acid synthesis. Deletion of S14 in mice decreased lipid synthesis in lactating mammary tissue, but the mechanism of S14's action is unknown. Here we present the crystal structure of S14 to 2.65 {angstrom} and biochemical data showing that S14 can form heterodimers with MIG12. MIG12 modulates fatty acid synthesis by inducing the polymerization and activity of acetyl-CoA carboxylase, the first committed enzymatic reaction in the fatty acid synthesis pathway. Coexpression of S14 and MIG12 leads to heterodimers and reduced acetyl-CoA carboxylase polymerization and activity. The structure of S14 suggests a mechanism whereby heterodimer formation with MIG12 attenuates the ability of MIG12 to activate ACC.

2011-09-06

157

A molecular model for the genetic and phenotypic characteristics of the mouse lethal yellow (A{sup y}) mutation

Attention deficit disorder (ADHD) is a complex biobehavioral phenotype which affects up to 8% of the general population and often impairs social, academic, and job performance. Its origins are heterogeneous, but a significant genetic component is suggested by family and twin studies. The murine strain, coloboma, displays a spontaneously hyperactive phenotype that is responsive to dextroamphetamine and has been proposed as a genetic model for ADHD. Coloboma is a semi-dominant mutation that is caused by a hemizygous deletion of the SNAP-25 and other genes on mouse chromosome 2q. To test the possibility that the human homolog of the mouse coloboma gene(s) could be responsible for ADHD, we have carried out linkage studies with polymorphic markers in the region syntenic to coloboma (20p11-p12). Five families in which the pattern of inheritance of ADHD appears to be autosomal dominant were studied. Segregation analysis of the traits studied suggested that the best ...

1995-12-18

158

Variational method for estimating the rate of convergence of Markov Chain Monte Carlo algorithms

Lethal yellow (A{sup y}) is a mutation at the mouse agouti locus in chromosome 2 that causes a number of dominant pleiotropic effects, including a completely yellow coat color, obesity, an insulin-resistant type II diabetic condition, and an increased propensity to develop a variety of spontaneous and induced tumors. Additionally, homozygosity for A{sup y} results in preimplantation lethality, which terminates development by the blastocyst stage. The A{sup y} mutation is the result of a 170-kb deletion that removes all but the promoter and noncoding first exon of another gene called Raly, which lies in the same transcriptional orientation as agouti and maps 280 kb proximal to the 3{prime} end of the agouti gene. The authors present a model for the structure of the A{sub y} allele that can explain the dominant pleiotropic effects associated with this mutation, as well as the recessive lethality, which is unrelated to the agouti gene.

1994-03-29

159

Twinning mechanism in PrCo_2C_x magnetic phase

We demonstrate the use of a variational method to determine a quantitative lower bound on the rate of convergence of Markov Chain Monte Carlo (MCMC) algorithms as a function of the target density and proposal density. The bound relies on approximating the second largest eigenvalue in the spectrum of the MCMC operator using a variational principle and the approach is applicable to problems with continuous state spaces. We apply the method to one dimensional examples with Gaussian and quartic target densities, and we contrast the performance of the basic Metropolis-Hastings algorithms with a ``smart'' variant that incorporates gradient information into the trial moves. We find that the variational method agrees quite closely with numerical simulations. We also see that the smart MCMC algorithm often fails to converge geometrically in the tails of the target density except in the simplest case we examine, and even then care must be taken to choose the appropriate ...

2006-01-01

160

Thermal performance study for the coal-fired combined cycle with partial gasification and fluidized bed combustion

A magnetic rhombohedral PrCo_2C_x (x = 0.05 #approx#0.25) phase (space group Rbar 3m), which is heavily twinned along the #left brace#110#right brace# and #left brace#211#right brace# planes, was identified. The twinning mechanism was explored by analyzing the reduction of crystal symmetry due to the cubic-rhombohedral phase transformation. The origin of the twinning and the formation of four twin variants were attributed to the insertion of carbon interstitials into Co_4 tetrahedrons along the bar 3 axis in the rhombohedral lattice, which corresponds to one of the four equivalent axes of its parent PrCo_2 cubic-lattice.

1997-04-04

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161

The cost effectiveness of cognitive behavioral therapy for bulimia nervosa delivered via telemedicine versus face-to-face

The coal-fired combined cycle with partial gasification and fluidized bed combustion (PGFBC-CC), also referred to as a hybrid cycle, has advantages of staged energy conversion and utilization, which can attain high thermal efficiency with low emissions. Four kinds of PGFBC-CC are studied in this paper, two based on pressurized fluidized bed combustion (PFBC) and two on atmospheric fluidized bed combustion (AFBC). Thermal performance calculations and parametric analyses were performed. On the basis of the results, from the above analyses, the best integration system for China is suggested. In addition, a preliminary exergetic analysis is carried out for three of the PGFBC-CC variants.

2001-07-01

162

British Library Electronic Table of Contents (United Kingdom)

Objective A number of effective treatments for bulimia nervosa have been developed, but they are infrequently used, in part due to problems with dissemination. The goal of this study was to examine the cost effectiveness of telemedicine delivery of cognitive behavioral therapy for bulimia nervosa. Method A randomized controlled trial of face-to-face versus telemedicine cognitive behavioral therapy for bulimia nervosa. One hundred twenty eight women with DSM-IV bulimia nervosa or eating disorder, not otherwise specified subsyndromal variants of bulimia nervosa were randomized to 20 sessions of treatment over 16 weeks. A cost effectiveness analysis from a societal perspective was conducted. Results The total cost per recovered (abstinent) subject was $9324.68 for face-to-face CBT, and $7300....

2009-01-01

163

The contribution of lower vertebrate animal models in human reproduction research

British Library Electronic Table of Contents (United Kingdom)

Many advances have been carried out on the estrogens, GnRH and endocannabinoid system that have impact in the reproductive field. Indeed, estrogens, the generally accepted female hormones, have performed an unsuspected role in male sexual functions thanks to studies on non-mammalian vertebrates. Similarly, these animal models have provided important contributions to the identification of several GnRH ligand and receptor variants and their possible involvement in sexual behavior and gonadal function regulation. Moreover, the use of non-mammalian animal models has contributed to a better comprehension about the endocannabinoid system action in several mammalian reproductive events. We wish to highlight here how non-mammalian vertebrate animal model research contributes to advancements with i...

2011-01-01

164

The Physics of Cosmic Acceleration

Study of questions of reliability of planning mining operations at open pits

The discovery that the cosmic expansion is accelerating has been followed by an intense theoretical and experimental response in physics and astronomy. The discovery implies that our most basic notions about how gravity work are violated on cosmological distance scales. One simple fix is the introduction of a cosmological constant into the field equations for general relativity. However, the extremely small value of the cosmological constant, relative to theoretical expectations, has led theorists to explore a wide variety of alternative explanations that involve the introduction of an exotic negative-pressure fluid or a modification of general relativity. Here we briefly review the evidence for cosmic acceleration. We then survey some of the theoretical attempts to account for it, including the cosmological constant, quintessence and its variants, mass-varying neutrinos, and modifications of general relativity, such as scalar-tensor and $f(R)$ theories and ...

2009-01-01

165

Studies on the diagnostic accuracy of lymphography

A method is proposed for evaluating reliability of planning and calculation of the reliability factor with current planning of open pit mining operations. For a quantitative evaluation of the reliability of fulfilling the planned assignment, laws were defined for distribution of the quality characteristics of the coal. After establishing that coal ash content follows the normal distribution law, the probability was defined that the actual output of coal is greater or equal to the planned. Calculation of the factor of reliability of the current planning of open mining operations makes it possible to determine the optimum planning for this period and the probability of fulfillment of the planned assignments. The technique of planning with regard for reliability makes it possible to select the most reliable variant of the plan within the planning period.

1982-01-01

166

Studies of human mutation rates

Contradictory reports in the literature on the reliability of lymphography stimulated the authors to test the diagnostic accuracy, employing methods which are approximately analogous to practice, using carcinoma of the cervix as the model on which the study was carried out. Using 21 observers it was found that there was no correlation between their experience and on-target accuracy of the diagnosis. Good observers obtained an accuracy of 85% with good proportions between sensitivity in the recognition of detail, specificity and readiness to arrive at a decision on the basis of discriminatory ability. With the help of the concept of the ROC curves, the position taken up by the observers in respect of diagnostic decisions, and a complex manner of assessing the various characteristic factors determining diagnostic accuracy, are demonstrated. This form of test, which permits manipulation of different variants of diagnosis, is recommended, among other things, for ...

167

Structures, Vibrational And Electronic Properties Of (F2O)N (N=2-4) Clusters

November 1989, marked the beginning of a new three-year cycle of DOE grant support, in connection with which the program underwent a major reorganization. This document presents the progress on the three objectives of the present program which are: to isolate by the technique of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), proteins of special interest because of the relative mutability of the corresponding gene, establish the identity of the protein, and, for selected proteins, move to a characterization of the corresponding gene; to develop a more efficient approach, based on 2-D PAGE, for the detection of variants in DNA, with special reference to the identification of mutations in the parents of the individual whose DNA is being examined; and, to continue an effective interface with the genetic studies on the children of atomic bomb survivors in Japan, with reference to both the planning and implementation of new studies at the molecular level.

1990-01-01

168

Rapid detection of drugs in biofluids using atmospheric pressure chemi/chemical ionization mass spectrometry

Recently, molecular clusters are the subject of several experimental and computational studies by means of their bonding structures. We studied, first time, small difluorine monoxide clusters such as dimer (linear, cyclic, bifurcated), trimer and tetramer structures using B3LYP variant of density functional theory with cc-pVDZ basis set. On the basis of the optimized geometry, various energy properties such as binding energy, molecular orbital energies, two and three body interaction energies have been calculated. Additionally dipole moment, polarizability, anisotropic polarizability and hyper polarizability have been calculated and compared with monomer structure.

2008-08-25

169

British Library Electronic Table of Contents (United Kingdom)

We have demonstrated that, with simple pH adjustment, volatile drugs such as methamphetamine, amphetamine, 3,4-methylenedioxymethamphetamine (MDMA), ketamine, and valproic acid could be analyzed rapidly from raw biofluid samples (e.g. urine and serum) without dilution, or extraction, using atmospheric pressure ionization. The ion source was a variant type of atmospheric pressure chemical ionization (APCI) that used a dielectric barrier discharge (DBD) to generate the metastable helium gas and reagent ions. The sample solution was loaded in a disposable glass pipette, and the volatile compounds were purged by nitrogen gas to be reacted with the metastable helium gas. The electrodes of the DBD were arranged in such a way that the generated glow discharge was confined within the discharge tub...

2009-01-01

170

Precipitation behavior and tensile property of the stress-aged Ti-10Mo-8V-1Fe-3.5Al alloy

British Library Electronic Table of Contents (United Kingdom)

A kind of metastable beta titanium alloy, i.e. Ti-10Mo-8V-1Fe-3.5Al, was aged under elastic compressive stress. Transmission electron microscopy (TEM) investigations revealed that, in the stress-aged samples, precipitated alpha plates were parallel to each other. However, in the conventionally aged samples, alpha plates intersected each other. In other words, variant of alpha plate was selected in the stress-aged samples. Tensile tests showed that, at equivalent tensile strength, the stress-aged samples exhibited higher ductility, compared to the conventionally aged ones.

2011-01-01

171

Non-destructive automated express method for determining the inclination of chromium-nickel steels IGC

Microcystins Induce Morphological and Physiological Changes in Selected Representative Phytoplanktons

Methods of automated control of 18-10-type steel inclination to IGC are developed and a corresponding automated testing complex (ATS) is created. 08Kh18N10T steel samples had two variants of thermal treatment: 1) 1200 deg (5 h), 600 deg (50 h); 2) 1200 deg (5 h). Methods of non-destructive automated control of 18-10-type steel inclination to IGC are developed on the basis of potentiodynamic reactivation (PR) principle. Automated testing complex is developed, which has undergone experimental running and demonstrated a high confidence of results, reliability and easy operation.

1986-01-01

172

British Library Electronic Table of Contents (United Kingdom)

Dissolved microcystins (MCs) are regularly present in water dominated by microcystin-producing, bloom-forming cyanobacteria. In vitro experiments with environmentally feasible concentrations (5 ? 10?7 M) of the three most common microcystins, MC-LR, MC-RR, and MC-YR, revealed that they influence the metabolism of different representative phytoplanktons. At light intensities that are close to the cyanobacterial bloom environment (50??mol m?2 s?1), they produce morphological and physiological changes in both microcystin-producing and -nonproducing Microcystis aeruginosa strains and also have similar effects on the green alga Scenedesmus quadricauda that is frequently present in cyanobacterial blooms. All three microcystin variants tested induce cell aggregation, increase in cell volume, and ...

2006-01-01

173

MA28: a set of Fortran subroutines for sparse unsymmetric linear equations. Revision

Introduction to the Special Issue: Human Linkage Studies for Behavioral Traits

In this report we present a suite of subroutines for the solution of sparse unsymmetric sets of linear equations using a variant of Gaussian elimination. The subroutines are divided into three distinct phases. The first phase optionally preorders the matrix to block triangular form and then performs a sparsity oriented factorization, the second factorizes a matrix of a similar sparsity pattern, while the third uses these decompositions to solve the equations. In this revised edition, the actual Fortran listings have been replaced by a reference to their availability in machine readable form. Other changes to the first edition are minor. This revision is essentially a reprint of the 1979 revision, the only changes being that the MA28A specification sheet has been typeset and has a few minor changes and the existence of a version of the package for complex equations is announced. 11 refs., 11 tabs.

1980-11-01

174

British Library Electronic Table of Contents (United Kingdom)

In the post Genome era, the aim of behavior genetics has shifted from estimating the relative contributions of genes and environmental factors to (co-)variation in human complex traits, to localization of genes and identification of functional genetic variants. This special issue reflects this transition and presents fifteen papers that report on genome-wide linkage scans for complex traits in humans and on methodological tools and innovations. Six papers focus on cognition and report overlapping linkage peaks on chromosomes 6p and 14p. Papers on addictive behavior, i.e. smoking and alcohol dependence and its endophenotypes, find moderate LOD scores on chromosomes 6p, 5q, 4p and 7q, respectively. Three papers concentrate on emotionality, depression and loneliness and examine chromosomes 2q...

2006-01-01

175

Importance of the Adrenal Gland Blood Supply During Laparoscopic Subtotal Adrenalectomy

British Library Electronic Table of Contents (United Kingdom)

Abstract Introduction: Laparoscopic subtotal adrenalectomy (LSA) has emerged as a treatment option in the management of certain adrenal pathologies. After LSA, a satisfactory blood supply to the adrenal remnant is vital to preserve steroid function. The aim of this study was to review the adrenal blood supply with relevance to LSA. Materials and Methods: The adrenal blood supply was examined in three ways: 1) a review of six previous cadaver studies looking at adrenal blood supply; 2) a review of our 164 consecutive laparoscopic adrenalectomies (LAs) (January 1999?January 2009); the details of the main adrenal veins and variants had been prospectively recorded; and 3) dissection of 4 cadaver specimens (macroscopic and histologic examination was performed). Results: The six cadaver studies ...

2010-01-01

176

Global Existence of Weak Solutions to a Nonlocal Cahn-Hilliard-Navier-Stokes System

Genome-wide association study identifies variants in the CFH region associated with host susceptibility to meningococcal disease

A well-known diffuse interface model consists of the Navier-Stokes equations nonlinearly coupled with a convective Cahn-Hilliard type equation. This system describes the evolution of an incompressible isothermal mixture of binary-fluids and it has been investigated by many authors. Here we consider a variant of this model where the standard Cahn-Hilliard equation is replaced by its nonlocal version. More precisely, the gradient term in the free energy functional is replaced by a spatial con- volution operator acting on the order parameter phi. Therefore the coupling with the Navier-Stokes equations is difficult to handle even in two spatial dimensions because of the lack of regularity of phi. We establish the global existence of a weak solution.

2011-01-01

177

British Library Electronic Table of Contents (United Kingdom)

Meningococcal disease is an infection caused by Neisseria meningitidis. Genetic factors contribute to host susceptibility and progression to disease, but the genes responsible for disease development are largely unknown. We report here a genome-wide association study for host susceptibility to meningococcal disease using 475 individuals with meningococcal disease (cases) and 4,703 population controls from the UK. We performed, in Western European and South European cohorts (consisting of 968 cases and 1,376 controls), two replication studies for the most significant SNPs. A cluster of complement factor SNPs replicated independently in both cohorts, including SNPs within complement factor H (CFH) (rs1065489 (p.936D

2010-01-01

178

Genetic fidelity of long-term micropropagated shoot cultures of vanilla (Vanilla planifolia Andrews) as assessed by molecular markers

British Library Electronic Table of Contents (United Kingdom)

Occurrence of genetic variants during micropropagation is occasionally encountered when the cultures are maintained in vitro for long period. Therefore, the micropropagated multiple shoots of Vanilla planifolia Andrews developed from axillary bud explants established 10 years ago were used to determine somaclonal variation using random amplified polymorphic DNA (RAPD) and intersimple sequence repeats markers (ISSR). One thousand micro-plants were established in soil of which 95 plantlets (consisting of four phenotypes) along with the mother plant were subjected to genetic analyses using RAPD and ISSR markers. Out of the 45 RAPD and 20 ISSR primers screened, 30 RAPD and 7 ISSR primers showed 317 clear, distinct and reproducible band classes resulting in a total of 30 115 bands. However, no ...

2007-01-01

179

GFAP in health and disease

British Library Electronic Table of Contents (United Kingdom)

Glial fibrillary acidic protein (GFAP) is the main intermediate filament protein in mature astrocytes, but also an important component of the cytoskeleton in astrocytes during development. Major recent developments in astrocyte biology and the discovery of novel intermediate filament functions enticed the interest in the function of GFAP. The discovery of various GFAP splice variants gave an additional boost to explore this protein in more detail.The structural role of GFAP in astrocytes has been widely accepted for a long time, but over the years, GFAP has been shown to be involved in astrocyte functions, which are important during regeneration, synaptic plasticity and reactive gliosis. Moreover, different subpopulations of astrocytes have been identified, which are likely to have distinc...

2011-01-01

180

Fente mediane cervicomaxillaire inferieure : a propos d'un cas, mise au point ethiopathogenique et therapeutique

British Library Electronic Table of Contents (United Kingdom)

Introduction: The orofacial clefts include 30 variant according to Tessier classification: the number 30 contain mandibular arc damage isolated or associated with damage of surrounding soft tissue. Case report: Our patient was a newborn with median mandibular cleft associated with ankyloglossia, bifid tongue and a top cervical fistula. We have not found polymalformative syndrome. The early surgical management included one time and after-effect were simple within 11months. Discussion: We point up difficulties for antenatal diagnosis and controversy about appropriate time for management of bone defect. The last physiopathologic hypotheses were explicated.

2011-01-01

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181

Experimental Comparison of Representation Methods and Distance Measures for Time Series Data

Disordered bcc γ-phase to δ-phase transformation in Zr-rich U-Zr alloy

The previous decade has brought a remarkable increase of the interest in applications that deal with querying and mining of time series data. Many of the research efforts in this context have focused on introducing new representation methods for dimensionality reduction or novel similarity measures for the underlying data. In the vast majority of cases, each individual work introducing a particular method has made specific claims and, aside from the occasional theoretical justifications, provided quantitative experimental observations. However, for the most part, the comparative aspects of these experiments were too narrowly focused on demonstrating the benefits of the proposed methods over some of the previously introduced ones. In order to provide a comprehensive validation, we conducted an extensive experimental study re-implementing eight different time series representations and nine similarity measures and their variants, and testing their effectiveness on ...

2010-01-01

182

British Library Electronic Table of Contents (United Kingdom)

The transformation mechanism of hexagonal delta phase from the disordered bcc gamma phase has not been reported before in the Zr-rich U-Zr alloy system. With the help of X-ray diffraction, transmission electron microscopy (TEM) and high-resolution TEM analyses it was shown that the gamma to delta conversion takes place by the lattice collapse mechanism of omega transformation. It was also ascertained that a higher aging temperature or time promotes the growth of all four variants of the delta phase within a parent gamma grain. In addition, ab initio electronic structure calculations showed that the bcc to hexagonal transformation, involving partial ordering of the parent bcc phase followed by (111) plane collapse, is energetically favorable.

2011-01-01

183

Crystal structure of the human centromeric nucleosome containing CENP-A

British Library Electronic Table of Contents (United Kingdom)

In eukaryotes, accurate chromosome segregation during mitosis and meiosis is coordinated by kinetochores, which are unique chromosomal sites for microtubule attachment. Centromeres specify the kinetochore formation sites on individual chromosomes, and are epigenetically marked by the assembly of nucleosomes containing the centromere-specific histone H3 variant, CENP-A. Although the underlying mechanism is unclear, centromere inheritance is probably dictated by the architecture of the centromeric nucleosome. Here we report the crystal structure of the human centromeric nucleosome containing CENP-A and its cognate ?-satellite DNA derivative (147 base pairs). In the human CENP-A nucleosome, the DNA is wrapped around the histone octamer, consisting of two each of histones H2A, H2B, H4 and CENP...

2011-01-01

184

Contribution to 3D-operational geodesy. Pt. 3

Consistency conditions in the chiral ring of super Yang-Mills theories

Whereas in part 1 and 2 the detailed observation equations of terrestrial type for an integrated geodetic model are presented this paper will outline how the theory is transferred in an operational program. The main lines of functions of the FORTRAN IV program are discussed. Since the input specifications as well as the data requirements are described in detail and illustrated by example of input and output part 3 can serve as an user-guide for OPERA. OPERA is an acronym for operational adjustment. Besides the integrated determination of 3D-geocentric coordinates and the gravity disturbing potential the program can handle all cases of traditional geodesy (in total 13 variants of solution are provided) equivalent also with adjustments in ellipsoidal coordinates B, L or H, as well as constrained adjustments, pure prediction of the gravity disturbing field, etc.

1983-01-01

185

Concordant Chemical Reaction Networks

Starting from the generalized Konishi anomaly equations at the non-perturbative level, we demonstrate that the algebraic consistency of the quantum chiral ring of the N=1 super Yang-Mills theory with gauge group U(N), one adjoint chiral superfield X and N_f<=2N flavours of quarks implies that the periods of the meromorphic one-form Tr dz/(z-X) must be quantized. This shows in particular that identities in the open string description of the theory, that follow from the fact that gauge invariant observables are expressed in terms of gauge variant building blocks, are mapped onto non-trivial dynamical equations in the closed string description.

2007-01-01

186

Characterization of All-Chromium Tunnel Junctions and Single Electron Tunneling Devices Fabricated by Direct-Writing Multilayer Technique

We describe a large class of chemical reaction networks, those endowed with a subtle structural property called concordance. We show that the class of concordant networks coincides precisely with the class of networks which, when taken with any weakly monotonic kinetics, invariably give rise to kinetic systems that are injective --- a quality that, among other things, precludes the possibility of switch-like transitions between distinct positive steady states. We also provide persistence characteristics of concordant networks, instability implications of discordance, and consequences of stronger variants of concordance. Some of our results are in the spirit of recent ones by Banaji and Craciun, but here we do not require that every species suffer a degradation reaction. This is especially important in studying biochemical networks, for which it is rare to have all species degrade.

2011-01-01

187

Cardiac Channelopathies and Sudden Infant Death Syndrome

We report about the fabrication and analysis of the properties of Cr/CrO_x/Cr tunnel junctions and SET transistors, prepared by different variants of direct-writing multilayer technique. In all cases, the CrO_x tunnel barriers were formed in air under ambient conditions. From the experiments on single junctions, values for the effective barrier height and thickness were derived. For the Cr/CrO_x/Cr SET transistors we achieved minimal junction areas of 17 x 60 nm^2 using a scanning transmission electron microscope for the e-beam exposure on Si_3N_4 membrane substrate. We discuss the electrical performance of the transistor samples as well as their noise behavior.

1999-01-01

188

British Library Electronic Table of Contents (United Kingdom)

Abstract Sudden infant death syndrome (SIDS) is always a devastating and unexpected occurrence. SIDS is the leading cause of death in the first 6 months after birth in the industrialized world. Since the discovery in 1998 of long QT syndrome as an underlying substrate for SIDS, around 10-20% of SIDS cases have been proposed as being caused by genetic variants in either ion channel or ion channel-associated proteins. Until now, 10 cardiac channelopathy susceptibility genes have been found to be implicated in the pathogenesis of SIDS. Four of the genes encode cardiac ion channel a-subunits, 3 genes encode ion channel b-subunits, and 3 genes encode other channel-interacting proteins. All 10 genes have been associated with primary electrical heart diseases. SIDS may hereby be the initial sympt...

2011-01-01

189

Analysis of the fatigue strength of welded joints produced by different techniques in materials of the type 'alloy 800'

Amino acid substitution: its use in detection and analysis of genetic variants

The alloy type 'alloy 800' with its different variants is used for numerous components in high-temperature reactor (HTR) plants, e.g. for hot-gas conductors, the high-pressure part of steam generators, superheaters, steam collecting vessels and live steam conductors. This study deals with the evaluation of creep rupture tests on 28 welded joints of alloy 800 with an accumulated test duration of 410 years, with the objective to obtain quantitative information on a possible loss of strength in comparison with the unwelded material. The longest test period of an individual test amounted to 98,000 h and the analyzed test temperature range lay between 550"0 and 1000"0C. (orig./MM).

1988-11-25

190

Alloy 800: New stress rupture and creep data for pressurised components in high-temperature reactors

Techniques of chemical analysis, amino acid sequencing and autoradiography are being used to study the frequency of incorporation of normally noncoded amino acids into hemoglobins and seminal fluid proteins. We are studying, by the sequencing of radiolabeled proteins followed by the recovery of ["3H] isoleucine phenylthiohydantoin by high-performance liquid chromatography, the frequency at which normally noncoded isoleucine is incorporated into hemoglobin because of base-substitution mutations versus translational errors. Irradiation increases the isoleucine content of human hemoglobin and the frequency of substitution of isoleucine for specific amino acids in rabbit hemoglobin. Studies to date indicate that these techniques have been developed sufficiently for initial analysis of the potential of drugs and environmental pollutants to induce base-substitution mutations in mammalian somatic cells.

191

Alloy 800 specifications in compliance with component requirements

A new evaluation of the 1% time strain limit for creep strength and the isochronous stress/strain relationship was to be undertaken for the type 800 alloy, based on up to date experimental data. After the evaluation of the 1% time strain limit curves and time fracture curves of individual melts, multilinear regression analyses were made to clear up the relationship between parameters of the creep behaviour such as creep strength, 1% time strain limit and time fracture strain on the one hand and metallurgical parameters such as C, Ti, Al, Ni, Cr contents or grain size and type of manufacture, on the other hand. Based on the knowledge gained from these calculations, new limits were determined for HTR material specifications for variants of the 800 alloy of the Rk, NT, DE, and HT type. (orig./DG).

1988-10-06

192

Alloy 800 specifications in compliance with component requirements

In view of the importance of the material Alloy 800 in high-temperature reactor plants (HTR), a material data bank was established which is used for statistical evaluation of mechanical and physical material behaviour. Based on investigations on the interconnection between the mechanical properties at high temperatures and the metallurgical parameters, different types of Alloy 800 were specified in compliance with the component requirements. In addition, aspects of corrosion and toughness behaviour were taken into consideration. The specifications and strength characteristics for the different variants of Alloy 800 were incorporated into draft DIN standards after discussion and approval in expert committees. Further important characteristics of the mechanical and physical material behaviour were summarized in HTR material data sheets so as to furnish an improved basis for the design and stress analyses of Alloy 800 components. (orig.).

1990-04-01

193

AFM Imaging of SWI/SNF action: mapping the nucleosome remodeling and sliding

194

A Unified Model for Evolutionary Multiobjective Optimization and its Implementation in a General Purpose Software Framework: ParadisEO-MOEO

We propose a combined experimental (Atomic Force Microscopy) and theoretical study of the structural and dynamical properties of nucleosomes. In contrast to biochemical approaches, this method allows to determine simultaneously the DNA complexed length distribution and nucleosome position in various contexts. First, we show that differences in the nucleo-proteic structure observed between conventional H2A and H2A.Bbd variant nucleosomes induce quantitative changes in the in the length distribution of DNA complexed with histones. Then, the sliding action of remodeling complex SWI/SNF is characterized through the evolution of the nucleosome position and wrapped DNA length mapping. Using a linear energetic model for the distribution of DNA complexed length, we extract the net wrapping energy of DNA onto the histone octamer, and compare it to previous studies.

2007-01-01

195

A Lamarckian Hybrid of Differential Evolution and Conjugate Gradients for Neural Network Training

This paper gives a concise overview of evolutionary algorithms for multiobjective optimization. A substantial number of evolutionary computation methods for multiobjective problem solving has been proposed so far, and an attempt of unifying existing approaches is here presented. Based on a fine-grained decomposition and following the main issues of fitness assignment, diversity preservation and elitism, a conceptual global model is proposed and is validated by regarding a number of state-of-the-art algorithms as simple variants of the same structure. The presented model is then incorporated into a general-purpose software framework dedicated to the design and the implementation of evolutionary multiobjective optimization techniques: ParadisEO-MOEO. This package has proven its validity and flexibility by enabling the resolution of many real-world and hard multiobjective optimization problems.

2009-01-01

196

British Library Electronic Table of Contents (United Kingdom)

The paper describes two schemes that follow the model of Lamarckian evolution and combine differential evolution (DE), which is a population-based stochastic global search method, with the local optimization algorithm of conjugate gradients (CG). In the first, each offspring is fine-tuned by CG before competing with their parents. In the other CG is used to improve both parents and offspring in a manner that is completely seamless for individuals that survive more than one generation. Experiments involved training weights of feed-forward neural networks to solve three synthetic and four real-life problems. In six out of seven cases the DE?CG hybrid, which preserves and uses information on each solution?s local optimization process, outperformed two recent variants of DE.

2010-01-01

197

Slide rings made of an SiC/silicide composite; Gleitringe aus einem SiC/Silicid-Verbundwerkstoff

Primary structures of four trypsin inhibitor E homologs from venom of Dendroaspis angusticeps: structure-function comparisons with other dendrotoxin homologs.

The objective of the project was to develop an infiltration material based on SiC that should have improved corrosion resistance and should permit higher operating temperatures. From a variety of tested doping agents, zirconium silicide and molybdenum proved to be the most appropriate agents. The respective infiltration materials permit a combination of advantages of the SSiC with those of the SiSiC. Silicide SiC, analogous to the SiSiC, is almost nonshrinking, and above all is more corrosion-resistant than SiSiC in the alkaline regime, due to the replacement of free silicon by silicide phases. The operating temperature of the molybdenum-base variant is 1600 C. (orig./CB) [Deutsch] Ziel des Vorhabens war die Entwicklung eines Infiltrationswerkstoffes auf SiC-Basis mit verbesserter Korrosionsbestaendigkeit und hoeherer Einsatztemperatur. Aus einer Vielzahl von getesteten Dotierungsmoeglichkeiten kristallisierten sich Zirkondisilicid und Molybdaen als geeingete ...

1997-12-31

198

Molecular events involved in ionizing radiation induced skin carcinogenesis

Four trypsin inhibitor homologs, the first known from Dendroaspis angusticeps venom, were characterized using a combination of gel filtration, cation exchange, reverse-phase liquid chromatography, Edman degradation and mass spectrometry. The four toxins comprise two 57 residue and two 59 residue isoforms. The long toxins possess a Lys-Gln N-terminal extension lacked by the short toxins. The only other structural difference is an Arg/His replacement at position 55. The long Arg55 variant is identical to trypsin inhibitor E from the venom of Dendroaspis polylepis. The name epsilon-dendrotoxin is suggested so as to follow the nomenclature of Benishin, C.G., Sorensen, R.G., Brown, W.E., Krueger, B.K., Blaustein, M.P., 1988. Four polypeptide components of green mamba venom selectively block certain potassium channels in rat brain synaptosomes. Mol. Pharmacol. 34, 152-159. Among snake venom protease inhibitors, the epsilon-dendrotoxins are structurally most like the ...

2002-03-01

199

Basic aspects of the concept of reactor compartment (including damaged compartments) management during utilization of nuclear powered submarines -- High priority R and D

The process of mouse skin tumor formation is subdivided into three operational stages. These stages include initiation, promotion and progression. Ionizing radiation has been found to be a weak initiating agent in the production of malignant squamous cell carcinomas, a complete carcinogen and an agent effective in causing tumor progression. Four skin tumor histologies have been seen with ionizing radiation: benign papillomas, squamous (SCC) and basal (BCC) cell carcinomas and fibrosarcomas. Distinct non-ras transforming genes have been detected in radiation initiated SCCs. A benign papilloma cell line (308) was used as a model system to study ionizing radiation induced progression. A variant 308 cell line (308 10 Gy 5) derived by irradiation of the parental 308 cell has been characterized. The 308 10 Gy 5 cells unlike the parental 308 cells from malignant tumors in athymic nude mice upon subcutaneous injection. The variant 308 10 Gy 5 cells ...

200

Why Gabor Frames? Two Fundamental Measures of Coherence and Their Role in Model Selection

Large-scale decommissioning of Russian nuclear-powered submarines (NPS) and their utilization prospects gave rise to numerous complicated scientific and technical, as well as economic, problems. Problems of handling of radioactive equipment from the reactor compartments (RC) are among the vital ones, arousing a growing concern with the public. Without solution of the problems the processes of NPS utilization can not be considered completed. It involves potential hazard, for the environment both from NPS being paid up (temporal on-float storage) with unloaded spent nuclear fuel (SNF), and RC, cut from submarine hull, containing highly radioactive equipment and materials but no SNF. Diverse variations of the concept of reactor compartment handling of NPS subject to, utilization are possible, but, in principle, there are essentially two variants: (1) RC utilization directly in the course of NPS utilization, envisaging removal of radioactive equipment from the reactor ...

1996-03-10

1 2 3 4 5 6 7 8 9 10 11 12 13 14

201

User-friendly algorithms for estimating completeness and diversity in randomized protein-encoding libraries.

The problem of model selection arises in a number of contexts, such as subset selection in linear regression, estimation of structures in graphical models, and signal denoising. This paper generalizes the notion of "incoherence" in the existing literature on model selection and introduces two fundamental measures of coherence---termed as the worst-case coherence and the average coherence---among the columns of a design matrix. It utilizes these two measures of coherence to provide an in-depth analysis of two variants of a simple one-step thresholding (OST) algorithm for model selection and proves that OST is feasible for model selection as long as the design matrix obeys an easily verifiable property. One of the key insights offered by the ensuing analysis in this regard is that if the design matrix has reasonably small worst-case and average coherence then OST performs near-optimally when either (i) the energy of any nonzero entry of the signal is close to the ...

2010-01-01

202

Tribological initial processes on 100Cr6 roller bearing steel in fuel-lubricated point contact; Tribologische Initialprozesse auf 100Cr6-Waelzlagerstahl im kraftstoffgeschmierten Punktkontakt

Directed evolution of proteins depends on the production of molecular diversity by random mutagenesis. While a number of methods have been developed for introducing this diversity, the best ways to sample it are not always clear. Here we used simple statistics to analyse completeness and diversity in randomized libraries generated by oligonucleotide-directed mutagenesis, error-prone polymerase chain reaction (epPCR) and in vitro recombination of highly homologous sequences. For oligonucleotide-directed mutagenesis, we derive equations to estimate how complete a given library is expected to be and also to predict the size of library required to give a fixed probability of being 100% complete. We describe the statistical bases for computer programs which estimate the number of distinct variants represented in epPCR and shuffled libraries, dubbed PEDEL and DRIVeR, respectively. These programs allow the user to calculate (rather than guess) the diversity represented in ...

2003-06-01

203

Treatment and recycling of spent nuclear fuel. Actinide partitioning - Application to waste management

The authors investigated the development of tribochemical reaction layers and surface-level structural changes in a fuel-lubricated roller bearing steel 100Cr6 in the limiting friction range, using two different variants of surface treatment and heat treatment. Tribological experiments were carried out in a laboratory test stand with reversing sliding wear under varying loads and with a varying number of friction cycles. [German] Ziel der vorliegenden Arbeit war es, die Entstehung von tribochemischen Reaktionsschichten und oberflaechennahen Gefuegeaenderungen am Waelzlagerstahl 100Cr6 bei Kraftstoffschmierung im Grenzreibungsbereich fuer je zwei Oberflaechenbearbeitungen und Waermebehandlungen zu untersuchen. Dazu wurden tribologische Kurzversuche in einem Laborpruefstand mit reversierender Gleitbeanspruchung unter Variation der Last und der Anzahl der Reibzyklen durchgefuehrt. (orig.)

2000-08-01

204

Transition of coal industry of the USSR to full self-financing

subsequent to its in-reactor dwell time, spent fuel still contains large amounts of materials that are recoverable, for value-added energy purposes (uranium, plutonium), together with fission products, and minor actinides, making up the residues from nuclear reactions. The treatment and recycling of spent nuclear fuel, as implemented in France, entail that such materials be chemically partitioned. The development of the process involved, and its deployment on an industrial scale stand as a high achievement of French science, and technology. Treatment and recycling allow both a satisfactory management of nuclear waste to be implemented, and substantial savings, in terms of fissile material. Bolstered of late as it has been, due to spectacularly skyrocketing uranium prices, this strategy is bound to become indispensable, with the advent of the next generation of fast reactors. This Monograph surveys the chemical process used for spent fuel treatment, and its ...

2008-01-01

205

Thermodynamic properties of dicarbonyl rhodium o-semiquinonate complex whose crystals display photomechanical properties

Describes progress made with the new economic system in the USSR coal industry since full self-financing was introduced on 1 January 1989, pointing out that mining is now being carried out at greater depths than before (515 m in 1988, 457 m in 1981 on the average) and that an increasing proportion of coal is being produced by mines liable to coal and gas bursts (62.3% in 1988, 59.6% in 1981). More difficult conditions means that mining costs are increasing. Explains system of agreements between teams of workers and mine management regarding output and extraction costs. Reveals that on 1 January 1990 a new list of coal prices will include 90% price rises on average, as a result of need for mines to finance themselves. There are to be 2 variants of this price list: one containing standard nationwide prices, the other based on mining areas, taking into account different geological and other conditions. Concludes by emphasizing the need to improve productivity, by ...

1989-11-01

206

The role of crystallographic and geometrical relationships between #alpha# and #beta# phases in an #alpha#/#beta# titanium alloy

In an adiabatic low-pressure calorimeter, the temperature dependence of the standard molar heat capacity of paramagnetic dicarbonyl rhodium complex with o-semiquinone (CO){sub 2}Rh(SQ) has been determined in the range from T=(6 to 355)K mainly with an accuracy of about 0.3%. Over the ranges from T=(205 to 234) K, T=(266 to 315)K and T=(316 to 345)K physical transformations have been revealed and their enthalpies and entropies have been estimated. The experimental data were used to calculate the thermodynamic functions C{sub p,m}{sup o}/R,{delta}{sub 0}{sup T}H{sub m}{sup o}/(R.K),{delta}{sub 0}{sup T}S{sub m}{sup o}/R and {phi}{sub m}{sup o}={delta}{sub 0}{sup T}S{sub m}{sup o}-{delta}{sub 0}{sup T}H{sub m}{sup o}/T (where R is the universal gas constant) between T=(0 and 355)K. The fractal dimension D in the heat capacity function of the fractal variant of Debye heat capacity theory has been evaluated.

2006-06-15

207

The development of the high-temperature reactor. In memoriam Prof. Dr. rer.nat. Dr.-Ing. e.h. Rudolf Schulten

The present study has examined for #alpha#/#beta#-Ti alloys the relationship between the morphology and crystallography of Widmanstaetten plates of #alpha#-Ti in colonies within a prior grain of #beta#-Ti. Thus, optical metallography, scanning electron microscopy and transmission electron microscopy have been used to characterize the morphological features of the microstructure, whereas orientation-imaging microscopy (OM) and transmission electron microscopy (TEM) have been employed to reveal crystallographic information. It has been discovered that within a prior #beta#-Ti grain, although the growth direction of the Widmanstaetten plates in given colonies may differ by large angles from #alpha#-plates in other colonies, they may exhibit very close crystallographic relationships. For example, inclined #alpha#-plates may share common basal planes and be related by a rotation of #approx#10.5 deg. about the c-axis of the crystals. This phenomenon has been interpreted on the basis of ...

2003-09-15

208

The Waste Management Plan integration into Decommissioning Plan of the WWR-S research reactor from Romania

In the future even more than in the past, nuclear power will be indispensable in the present industrialized countries and in those still under development. The safe, nonpolluting, and economic supply of energy to mankind in the future includes so many different problems that the technology of the high-temperature reactor at its present level of development, and with the possibilities is offers above and beyond those provided by other, established, technologies, does not have to mark the end of some old line of development, but rather should be seen as the starting point of a development offering promise for the future. It is for this very reason that the extensive, valuable knowledge and experience accumulated in the construction, operation, and decommissioning of the AVR and THTR plants, the development of the HTR module and other variants and, last, but not least, the valuable results of projects such as PNP, NFE, and HHT, must be preserved at the research ...

209

TMPRSS6 rs855791 modulates hepcidin transcription in vitro and serum hepcidin levels in normal individuals.

The paper presents the progress of the Radioactive Waste Management Plan which accompanies the Decommissioning Plan for research reactor WWR-S located in Magurele, Ilfov, near Bucharest, Romania. The new variant of the Decommissioning Plan was elaborated taking into account the IAEA recommendation concerning radioactive waste management. A new feasibility study for WWR-S decommissioning was also developed. The preferred safe management strategy for radioactive wastes produced by reactor decommissioning is outlined. The strategy must account for reactor decommissioning, as well as rehabilitation of the existing Radioactive Waste Treatment Plant and the upgrade of the Radioactive Waste Disposal Facility at Baita-Bihor. Furthermore, the final rehabilitation of the laboratories and reusing of cleaned reactor building is envisaged. An inventory of each type of radioactive waste is presented. The proposed waste management strategy is selected in accordance with the IAEA ...

2008-05-28

210

Synthesis of alkylated deoxyno irimycin and 1,5-dideoxy-1,5-iminoxylitol analogues: : Polar side-chain modification, sulfonium and selenonium heteroatom variants, conformational analysis, and evaluation as glycosidase inhibitors

The iron hormone hepcidin is inhibited by matriptase-2, a liver serine-protease encoded by TMPRSS6 gene. Cleaving the BMP-coreceptor hemojuvelin, matriptase-2 impairs the BMP/SMAD signaling pathway, downregulates hepcidin and facilitates iron absorption. TMPRSS6 inactivation causes iron-deficiency-anemia refractory to iron administration both in humans and mice. Genome wide association studies have shown that the SNP rs855791, which causes the matriptase-2 V736A amino acid substitution, is associated with variations of serum iron, transferrin saturation, hemoglobin and erythrocyte traits. Here we show that in vitro matriptase-2 736(A) inhibits hepcidin more efficiently than 736(V). Moreover, in a genotyped population, after exclusion of samples with iron deficiency and inflammation, hepcidin, hepcidin/transferrin saturation and hepcidin/ferritin ratios were significantly lower and iron parameters were consistently higher in homozygotes 736(A) than in 736(V). Our results indicate that ...

2011-08-26

211

Some medical applications of thermomechanically treated titanium nickelide

The syntheses of N-alkylated deoxynojirimycin and 1,5-dideoxy-1,5-iminoxylitol derivatives having either a D- or an L-erythritol-3-sulfate functionalized N-substituent are reported. The alkylating agent used was a cyclic sulfate derivative, whereby selective attack of the nitrogen atom at the least hindered primary center afforded the desired ammonium salt. In aqueous solution, these salts were configurationally labile at the ammonium center. Sulfonium and/or selenonium analogues of the ammonium salts were prepared by analogous reactions. The chalcogen salts were obtained as mixtures of diastereomers, separable in some cases, differing only in the stereochemistry at the configurationally stable sulfur or selenium atoms. Proof of configuration and conformation of each compound was obtained by detailed NMR experiments. The compounds are six-membered ring analogues of salacinol, a known sulfonium-salt glucosidase inhibitor. Evaluation of the target compounds for enzyme inhibition of the ...

2004-01-01

212

Results of in-house cone-cup testing of low to high temperature SPF-alloys

An original device and a method of its application for restoring of the function of relatively incompetent valves (both patented) are elaborated. Application of the new device allows to lower the difficulty of surgical treatment, to decrease the duration of operation and post-operative period. The long-term results of six-year long experience of its application are presented. The patients examination after 2,5-3,0-year post-operation period shows perfect vein valve correction. A device for stone extraction from tubular organs (patented) fabricated with titanium nickelide superelastic alloy is presented. The new suggested design is free of the drawback inherent in the previous one. The working element of the device is formed as a truncated cone or a truncated cone coaxial with the cylinder (the previous design was formed as a full cone) that prevents overstraining and residual strain accumulation during the manipulation process. Since the first publication the design of spiral stent and ...

2001-11-01

213

Resolving the Pu Electronic Structure Enigma: Past Lessons and Future Directions

Material testing of SPF-sheet is usually done with coupon hot tensile testing. The optimum range of strain rates is determined with stepped strain tests. To overcome the problem of very few available testing facilities and time consuming and costly procedure, cone cup testing has been developed since long. This test can be generated directly in the workshop, no machining of samples is necessary and it is very much related to the real SPF-process. The analysis of suitable temperature and strain rate is done by simple measurement of resulting cone height. This paper describes a unique and novel variant of the above mentioned baseline design. It provides on-line data of the actual status of the cone cup forming resulting from the applied, constant pressure. Strain rate variations as a result from texture or from the alloy-related behaviour is detectable. Thinning and ''hardening'' behaviour of different alloys up to maximum failure ...

2004-07-01

214

Online monitoring of power transformers - a new low-cost system for small and medium power transformers; Monitoring von Leistungstransformatoren - Jetzt auch fuer kleine und mittlere Baugroessen

The nature of the 5f electronic structure of plutonium (Pu) remains unclear. [1] Despite many recent attempts to resolve the issue, a plethora of important unanswered questions remain. While many theoretical approaches to the problem have been promulgated, the real source of the difficulty is the absence of sufficient experimental benchmarking. This paucity of impact on the part of experimental results is driven in part by the difficulties of working with Pu: it is highly radioactive, biologically toxic, chemically reactive and restricted in its distribution and permitted access to user facilities. The results of these liabilities include the following: (1) it is very difficult, if not impossible, to get large single crystals of single phase samples and; (2) many state of the art experiments can not be done because general user facilities are not available for use with Pu samples. Additionally, there is the apparently bizarre nature of Pu, which seems to defeat the efforts to analyze ...

2008-05-30

215

Non local theories: New rules for old diagrams

Until now, monitoring of power transformers focussed mainly large generator and network transformers. Siemens goes a step ahead and provides a system especially developed and designed for small and medium power transformers, e.g. for the 100 kV level. This low cost version could be achieved by restriction to really necessary measuring quantities. This allows to realize also for small and medium power transformers an online monitoring including an estimation of aging and as an option an early warning to avoid unexpected failures. (orig.) [German] Die Ueberwachung (Monitoring) von Leistungstransformatoren war bisher vor allem fuer Maschinentransformatoren und grosse Netzkuppeltransformatoren in der Diskussion. Siemens geht einen Schritt weiter und stellt nun auch ein System bereit, das speziell fuer kleine und mittlere Leistungstransformatoren, z.B. der 110-kV-Spannungsebene, konzipiert ist. Diese kostenguenstige Variante wurde durch Beschraenkung auf die aus ...

1999-09-20

216

Neutron irradiation of superconducting compounds

We show that a general variant of the Wick theorems can be used to reduce the time ordered products in the Gell-Mann and Low formula for a certain class on non local quantum field theories, including the case where the interaction Lagrangian is defined in terms of twisted products. The only necessary modification is the replacement of the Stueckelberg-Feynman propagator by the general propagator (the 'contractor' of Denk and Schweda) D(y-y';#tau#-#tau#')=(1)/i(#DELTA#_+(y-y')#theta#(#tau#-#tau#')+#DELTA#+(y'-y)#theta#(#tau#'-#tau#)), where the violations of locality and causality are represented by the dependence of #tau#,#tau#' on other points, besides those involved in the contraction. This leads naturally to a diagrammatic expansion of the Gell-Mann and Low formula, in terms of the same diagrams as in the local case, the only necessary modification concerning the Feynman rules. The ordinary local theory is easily recovered as a special case, and there is a ...

2004-08-01

217

Monte-Carlo-based simulation of LWR cores with innovative fuel concepts

The effects of neutron irradiation on the superconducting and normal state properties of alloys and compounds are presented. Particular emphasis is placed on the A-15 compounds where the effects of neutron irradiation on Tsub(c), Hsub(c_2), long range order parameter and lattice parameter are described. Large depressions (up to 80%) in Tsub(c) are observed for all the A-15 compounds studied with the exception of Mo_3Os where much smaller decreases in Tsub(c) are seen. Along with the decrease in Tsub(c) and increase in lattice parameter, the degree of long range order, as measured by X-ray and neutron diffraction, decreases. Also presented are the results of isothermal and isochronal anneals up to 900"0C. The unirradiated value of Tsub(c) can be restored by annealing, and for those systems where measurements have been made, recovery of the lattice parameter and order parameter also takes place. The effects observed in irradiated material, together with those observed in unirradiated but ...

218

MRI in preoperative evaluation of chronic paranasal sinusitis - a comparison with CT

High resolution Monte-Carlo simulations show that the neutron spectrum, fuel burnup and fuel temperature feedback effect of a PWR core loaded with Thoria-based fuel (Th/Pu-O_2) do not significantly differ from the MOX fuelled one due to the similar neutronic characteristics of both fertile materials (Th-232, U-238). The core physics of this fuel variant is characterized by an enhanced moderator/void temperature coefficient (by factor 2.4) and high incineration rate for Pu (approx. 60 %). A PWR core loaded with the Molybdenum-based inert matrix fuel (IMF) - in contrast to MOX-, shows a harder spectrum, resulting in small temperature coefficients of reactivity and particularly in a higher fuel depletion rate as well as an enhanced TRU reduction performance. The incineration of Pu amounts to 46 % resulting, in turn, in generation of minor actinides of about 10 % of the total Pu consumption. The higher excess reactivity resulting from the initial Pu contents is ...

2009-05-03

219

Light-dependent regulation of DEL1 is determined by the antagonistic action of E2Fb and E2Fc.

Purpose: To determine the value of MRI in the preoperative evaluation of chronic paranasal sinusitis and to compare the results with CT and intraoperative findings. Method/Materials: 42 patients with clinical signs of chronic paranasal sinusitis underwent MRI after CT evaluation on one day, with subsequent functional endoscopic sinus surgery on the next day. Coronary CT was obtained with 5-mm slices and table-feed in the prone position, while MRI was performed in the supine position with coronary T_2-TSE+pd and coronary and transverse HASTE, each with 5-mm slice thickness. Aquisition time in MRI was less than 8 minutes. Two radiologists reviewed the CT and MRI scans for signs of sinusitis and detection of anatomical landmarks. The results were correlated with the intraoperative findings. Results: MRI offered no artifacts of dental work and showed more often high quality pictures than CT. CT and MRI demonstrated a good correlation in the detection of mucosal pathologies ...

2001-04-01

220

Input of cogeneration units in the future Dutch electric power supply. Inzet van warmte/krachteenheden in de toekomstige Nederlandse elektriciteitsvoorziening

Endoreduplication represents a cell cycle variant during which multiple rounds of DNA replication occur without subsequent chromosome separation and cytokinesis, resulting into a cellular increase of the DNA content. Although the DNA ploidy level of cells is controlled by external stimuli such as light, currently limited knowledge is available on how environmental signals regulate the endoreduplication cycle at the molecular level. Previously, we have demonstrated that the conversion from the mitotic cell cycle into an endoreduplication cycle is mediated by the atypical E2F transcription factor DEL1 that operates as a repressor of endocycle onset. Here, we identified DEL1 as a transcriptional target of the classical E2Fb and E2Fc transcription factors that antagonistically control DEL1 transcript levels through competition for a single E2F cis-acting binding site. Correspondingly with the reported opposite effect of light on the E2Fb and E2Fc protein levels, the ...

2011-09-01

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221

Impact of genetic polymorphisms in ABCB1, CYP2B6, OPRM1, ANKK1 and DRD2 genes on methadone therapy in Han Chinese patients

An investigation was carried out to determine the significance of a large input of cogeneration units for the electric power generation, next to the input of coal and uranium. Thereto a production plant has been set up to generate heat and electric power. The plant serves as a basis for a number of comparative calculations: basic calculations to investigate the influence of some strategies on the cogeneration input, and sensitivity calculations to determine the influence of a few changes in the starting points. Some variants have been deduced from this plant, which is based on prognosis for the year 2000. By means of the computer calculation program OPINIE (Optimale Inzet en Instelling van Eenheden: Optimal Input and Installation of Units) the minimal variable costs were calculated for different situations. Finally for each considered situation the total annual costs have been calculated. 53 figs., 13 refs., 30 tabs., 3 apps.

1987-02-01

222

British Library Electronic Table of Contents (United Kingdom)

Aims: The present study explored the integrative effect of genes encoding methadone pharmacokinetic and pharmacodynamic pathways on methadone maintenance doses in Han Chinese Patients. Materials & methods: Genomic DNA was extracted from 321 opioid-dependent patients and 202 healthy controls, and realtime-PCR and PCR-RFLP were conducted to determine the genotypes. Results: Pair-wise comparisons revealed that carriers of the variants ABCB1 3435C>T or CYP2B6 516G>T alleles were more likely to require a higher methadone dose than noncarriers (both p G or 939C>T allele had a two-fold chance of requiring a lower methadone dose than noncarriers (p = 0.001). Proportional odds regression with adjustment of cofactors demonstrated that ...

2011-01-01

223

Hemoglobin of mice with radiation-induced mutations at the hemoglobin loci

Global and trajectory attractors for a nonlocal Cahn-Hilliard-Navier-Stokes system

Chemical analyses were done on the abnormal hemoglobins of the five (101 x SEC)F_1 offspring of X- irradiated adult SEC mice to determine which hemoglobin genes were expressed in each hemoglobin variant. Three offspring of irradiated SEC males did not express either of the two kinds of #alpha#-chains normally found in all SEC mice. The deficient #alpha#-chain synthesis caused these mice to exhibit an #alpha#-thalassemia similar to human #alpha#-thalassemia. Scanning electron microscopy was used to show that many erythrocytes of mice with #alpha#-thalassemia have bizarre shapes; e.g. many erythrocytes appeared flattened or had thorny projections (acanthocytes). One mutant with a tandem duplication of a segment of chromosome 7 (site of locus determining #beta#-chain structure) produced twice as much SEC as 101 #beta#-chain polypeptides. One mutant that probably arose by non-disjunction of chromosome 7's in its unirradiated 101 mother and loss of chromosome 7 from the ...

224

Focal lesions in external auditory canal: computed tomography images

The Cahn-Hilliard-Navier-Stokes system is based on a well-known diffuse interface model and describes the evolution of an incompressible isothermal mixture of binary fluids. A nonlocal variant consists of the Navier-Stokes equations suitably coupled with a nonlocal Cahn-Hilliard equation. The authors, jointly with P. Colli, have already proven the existence of a global weak solution to a nonlocal Cahn-Hilliard-Navier-Stokes system subject to no-slip and no-flux boundary conditions. Uniqueness is still an open issue even in dimension two. However, in this case, the energy identity holds. This property is exploited here to define, following J.M. Ball's approach, a generalized semiflow which has a global attractor. Through a similar argument, we can also show the existence of a (connected) global attractor for the convective nonlocal Cahn-Hilliard equation with a given velocity field, even in dimension three. Finally, we demonstrate that any weak solution fulfilling ...

2011-01-01

225

Effect of low copper content and heat treatment on intergranular corrosion of model AlMgSi alloys

To analyze and review the most common focal lesions of the external auditory canal (EAC), describing the computed tomography (CT) findings. The CT images of EAC were reviewed in a series of 167 patients. Abnormal findings were recorded in 13 cases. The CT images were correlated with otoscopic and/or intraoperative findings. There were four cases of osteoma (one located outside the EAC); all of which were treated surgically, as was the one case of exostosis. Six patients presented debris in EAC, coinciding with clinical evidence of external otitis. There were two cholesteatomas derived from EAC, one of them secondary to survery to treat osteoma. On patient was diagnosed as having a basal cell carcinoma of the ear, extending to EAC, but refused to undergo surgery. Five patients presented small triangular laminae of dense bone arising from the upper wall of the EAC which were defined as abnormal variants of the fusion of the temporal squamous epithelium to the ...

226

Dynamic User-Defined Similarity Searching in Semi-Structured Text Retrieval

Certain 6000-series extrusions may develop susceptibility to intergranular corrosion (IGC) by improper heat treatment, especially if copper is present as an alloying element. Although occurrence of IGC in such cases is documented, the underlying mechanisms are not adequately explained. We present corrosion data for two model alloys, having different Cu content and Mg:Si ratio, showing that the susceptibility to IGC depended primarily on the Cu content and secondly on thermal processing. Low Cu samples (0.0005 wt.% Cu) were essentially resistant to IGC. High Cu samples (0.12 wt.% Cu), which were air cooled after extrusion, exhibited significant IGC. However, IGC susceptibility was reduced significantly as a result of artificial aging to peak strength. Water quenched high Cu samples were essentially resistant to IGC. However, slight IGC susceptibility was introduced after aging. Electron optical characterisation revealed Al_4Mg_8Si_7Cu_2 (Q-phase) grain boundary precipitates on all the ...

2006-01-01

227

Creep-rupture, creep strength and tensile data on Alloy 800 between 500-800"0C

Modern text retrieval systems often provide a similarity search utility, that allows the user to find efficiently a fixed number k of documents in the data set that are most similar to a given query (here a query is either a simple sequence of keywords or the identifier of a full document found in previous searches that is considered of interest). We consider the case of a textual database made of semi-structured documents. Each field, in turns, is modelled with a specific vector space. The problem is more complex when we also allow each such vector space to have an associated user-defined dynamic weight that influences its contribution to the overall dynamic aggregated and weighted similarity. This dynamic problem has been tackled in a recent paper by Singitham et al. in in VLDB 2004. Their proposed solution, which we take as baseline, is a variant of the cluster-pruning technique that has the potential for scaling to very large corpora of documents, and is far ...

2007-01-01

228

Common colorectal cancer risk variants in SMAD7 are associated with survival among prediagnostic nonsteroidal anti-inflammatory drug users: A population-based study of postmenopausal women

The authors present tensile data and creep-rupture data out to approximately 40,000 h duration on low-carbon Alloy 800. Each of the four casts was heat treated to three conditions in tube form. When the test programme was originally conceived, the prime object was to obtain 100,000 hour design data; the programme was designed prior to some of the more recently discovered effects such as that of #gamma#' on ductility. Therefore no specific attempt was made to control to a low level, the Al and Ti contents of the casts. A subsequent programme was embarked upon in 1976 where these factors were taken into account. The bulk data obtained show many characteristics which are probably typical of the low-carbon variant of this material and both the quantity and duration of the data are a contribution to the knowledge of the long-term behaviour of this class of alloy, whose future use lies, inter alia, in nuclear applications over the temperature range for which ERA data ...

229

British Library Electronic Table of Contents (United Kingdom)

Abstract Common single nucleotide polymorphisms (SNPs) in SMAD7 (18q21) have been linked to colorectal cancer (CRC) risk in genome-wide association studies, but little is known about their effects on survival. SMAD7 regulates gastrointestinal inflammation by inhibiting transforming growth factor- (TGFB), which can act as both a tumor suppressor and a promoter of metastasis. Regular use of cyclooxygenase-2 (COX2) inhibitors, such as nonsteroidal anti-inflammatory drugs (NSAIDs), reduces the risk of developing CRC. Because COX2 overexpression reduces the growth suppressing effects of TGFB, we hypothesized that survival may depend on both SMAD7 genotype and prediagnostic NSAID use. Postmenopausal women, ages 50-74, diagnosed with incident invasive CRC from 1997 to 2002 were identified using t...

2011-01-01

230

An adaptive filter to approximate the Bayesian strategy for sonographic beamforming.

A first-principles task-based approach to the design of medical ultrasonic imaging systems for breast lesion discrimination is described. This study explores a new approximation to the ideal Bayesian observer strategy that allows for object heterogeneity. The new method, called iterative Wiener filtering, is implemented using echo data simulations and a phantom study. We studied five lesion features closely associated with visual discrimination for clinical diagnosis. A series of human observer measurements for the same image data allowed us to quantitatively compare alternative beamforming strategies through measurements of visual discrimination efficiency. Employing the Smith-Wagner model observer, we were able to breakdown efficiency estimates and identify the processing stage at which performance losses occur. The methods were implemented using a commercial scanner and a cyst phantom to explore development of spatial filters for systems with shift-variant ...

2010-07-19

231

Amyloidosis

Transcription regulation of the vegf gene by the BMP/Smad pathway in the angioblast of zebrafish embryos

The subjects covered in this Symposium range through almost every clinical medical specialty. From an average of one paper in each of the past three Symposiums, the explosive interest in cerebral amyloidosis has led to the presentation of 12 papers on this subject in the present volume. The genetically predisposed familial amyloidotic processes, such as the polyneuropathies and familial Mediterranean fever have also stimulated extensive and intriguing investigations which have revealed the striking effect of a single amino acid substitution in transforming a normal protein into a lethal ''amyloidogenic'' one. This Symposium clearly depicts the advances since the first amyloid fibril protein was definitively identified and defined 14 years ago. Since all amyloid fibril proteins so far described are variants of normal proteins, attention to gene abnormalities now becomes a significant focus as well as the pathogenic sequences which lead in these cases to twisted ...

1984-11-09

232

The neuronal nicotinic acetylcholine receptor {alpha}7 subunit gene: Cloning, mapping, structure, and targeting in mouse

Vascular endothelial growth factor (VEGF) is a mitogen that is critically involved in vasculogenesis, angiogenesis, and hematopoiesis. However, what and how transcription factors participate in the regulation of vegf gene expression are not fully understood. Here we report the cloning and sequencing of the zebrafish vegf promoter which revealed that the promoter contains a number of bone morphogenetic protein (BMP)-activated Smad binding elements (SBE), implicating Smad1 and Smad5 in the regulation of BMP-induced expression of vegf. Electrophoretic mobility shift assays of adding recombinant Smad proteins to the SBE-containing DNA oligonucleotides that represent portions of zebrafish vegf promoter resulted in mobility shift of the oligonucleotides. These changes demonstrate potential interactions between Smad1/5 and the vegf promoter. Reporter activity assays using the wild-type or SBE-deleted vegf promoters to drive the luciferase reporter gene expression revealed ...

2005-04-01

233

Radiation accidents in the Southern Urals (1949-1967) and human genome damage.

The neuronal nicotinic acetylcholine receptor {alpha}7 subunit is a member of a family of ligand-gated ion channels, and is the only subunit know to bind {alpha}-bungarotoxin in mammalian brain. {alpha}-Bungarotoxin binding sites are known to be more abundant in the hippocampus of mouse strains that are particularly sensitive to nicotine-induced seizures. The {alpha}7 receptor is highly permeable to calcium, which could suggest a role in synaptic plasticity in the nervous system. Auditory gating deficiency, an abnormal response to a second auditory stimulus, is characteristic of schizophrenia. Mouse strains that exhibit a similar gating deficit have reduced hippocampal expression of the {alpha}7 subunit. We have cloned and sequenced the full length cDNA for the mouse {alpha}7 gene (Acra-7) and characterized its gene structure. The murine {alpha}7 shares amino acid identity of 99% and 93% with the rat and human {alpha}7 subunits, respectively. Using an interspecies backcross panel, the ...

1994-09-01

234

Nuclear Forensics and Attribution for Improved Energy Security: The Use of Taggants in Nuclear Fuel

A series of radioactive catastrophes (from 1948 to 1967) in the Southern Urals in the USSR led to intensive environmental contamination. Radioactive wastes were dispersed over the 20000 km(2) territory of four provinces-Chelyabinsk, Sverdlovsk, Tyumen' and Kurgan-due to the activity of the military facility that was built in 1948 for the production of nuclear bomb plutonium. The results of 50 years of investigations into the consequences of these disasters allow a general picture of the events that occurred to be reconstructed and allow the medical consequences of the irradiation of about half a million residents to be depicted. However, due to the atmosphere of secrecy and inadequate medical procedures, the results of medical studies of radiation victims are scant. The current protocols present a unique opportunity to study the DNA damage at the nucleotide resolution level in the genome of inhabitants of the given region, who presumably received chronic doses of irradiation. Studies ...

2002-11-01

235

Murine antibody response to oral infection with live aroA recombinant Salmonella dublin vaccine strains expressing filamentous hemagglutinin antigen from Bordetella pertussis.

The Global Nuclear Energy Partnership (GNEP), recently announced by DOE Secretary Bodman, poses significant new challenges with regard to securing, safeguarding, monitoring and tracking nuclear materials. In order to reduce the risk of nuclear proliferation, new technologies must be developed to reduce the risk that nuclear material can be diverted from its intended use. Regardless of the specific nature of the fuel cycle, nuclear forensics and attribution will play key roles to ensure the effectiveness of nonproliferation controls and to deter the likelihood of illicit activities. As the leader of the DHS nuclear and radiological pre-detonation attribution program, LLNL is uniquely positioned to play a national leadership role in this effort. Ensuring that individuals or organizations engaged in illicit trafficking are rapidly identified and apprehended following theft or diversion of nuclear material provides a strong deterrent against unlawful activities. Key to establishing this ...

236

Insulin-like growth factor binding protein-7 (IGFBP7) functions as a potential tumor suppressor in hepatocellular carcinoma (HCC).

Two plasmids which express either nearly intact or truncated filamentous hemagglutinin (FHA) from Bordetella pertussis and which are marked with a tetracycline resistance (Tcr) gene were transformed into Salmonella dublin SL1438, an aroA deletion mutant intended for use as an attenuated oral vaccine against salmonellosis. These S. dublin recombinants, when fed to mice, induced serum immunoglobulin, immunoglobulin M (IgM), and sometimes IgA antibody responses to FHA and S. dublin. In addition, IgA antibodies against FHA were found in gut wash fluids. S. dublin carrying pDB2300, a multicopy plasmid encoding truncated FHA protein, induced a better antibody response than did S. dublin carrying pDB2000, a low-copy-number plasmid encoding full-sized FHA. Administration of tetracycline to mice enhanced the stability of recombinant plasmids, and tetracycline-treated mice developed higher anti-FHA titers. Although neither strain examined is suitable for use in a human oral ...

1990-08-01

237

Gene expression profiles deciphering rice phenotypic variation between Nipponbare (Japonica) and 93-11 (Indica) during oxidative stress.

PURPOSE: Hepatocellular carcinoma (HCC) is a highly virulent malignancy with no effective treatment thus requiring innovative and effective targeted therapies. The oncogene Astrocyte elevated gene-1 (AEG-1) plays a seminal role in hepatocarcinogenesis and profoundly downregulates insulin-like growth factor binding protein-7 (IGFBP7). The present study focuses on analyzing potential tumor suppressor functions of IGFBP7 in HCC and the relevance of IGFBP7 downregulation in mediating AEG-1 function.EXPERIMENTAL DESIGN: IGFBP7 expression was detected by immunohistochemistry in HCC tissue microarray and real-time PCR and ELISA in human HCC cell lines. Dual Fluorescence in situ hybridization was performed to detect loss of heterozygosity at IGFBP7 locus. Stable IGFBP7-overexpressing clones were established in the background of AEG-1-overexpressing human HCC cells and were analyzed for in vitro proliferation and senescence and in vivo tumorigenesis and angiogenesis.RESULTS: IGFBP7 expression ...

2011-09-16

238

Ectopic expression of a COOH-terminal fragment of the human telomerase reverse transcriptase leads to telomere dysfunction and reduction of growth and tumorigenicity in HeLa cells.

Rice is a very important food staple that feeds more than half the world's population. Two major Asian cultivated rice (Oryza sativa L.) subspecies, japonica and indica, show significant phenotypic variation in their stress responses. However, the molecular mechanisms underlying this phenotypic variation are still largely unknown. A common link among different stresses is that they produce an oxidative burst and result in an increase of reactive oxygen species (ROS). In this study, methyl viologen (MV) as a ROS agent was applied to investigate the rice oxidative stress response. We observed that 93-11 (indica) seedlings exhibited leaf senescence with severe lesions under MV treatment compared to Nipponbare (japonica). Whole-genome microarray experiments were conducted, and 1,062 probe sets were identified with gene expression level polymorphisms between the two rice cultivars in addition to differential expression under MV treatment, which were assigned as Core Intersectional Probesets ...

2010-01-08

239

Characterization of the human lipoprotein lipase (LPL) promoter: Evidence of two cis-regulatory regions, LP-[alpha] and LP-[beta] of importance for the differentation-linked induction of the LPL gene during adipogenesis

The COOH-terminus of telomerase reverse transcriptase (hTERT) has been shown to participatein the nuclear translocation of TERT. Here, we constructed plasmids expressing the COOH-terminal M(r) 27,000 polypeptide of hTERT (hTERTC27) withthe telomerase RNA-binding domains and the reverse transcriptase domains deleted. We showed that ectopic overexpression of this polypeptide caused a defect in telomere maintenance in hTERT-positive HeLa cells, which led to senescence-like growth arrest and apoptosis. The hTERTC27 appears to work by inducing telomere dysfunction, exemplified by significantly increased anaphase chromosome end-to-end fusion events in transfected cells. Significantly, it had no effect on the cellular telomerase enzymatic activity or telomere length. The in vivo effect was further demonstrated as HeLa cells stably expressing hTERTC27 have significantly lower growth rate and reduced tumorigenicity in nude mice xenografts. Results from this study revealed ...

2002-06-01

240

Aarskog-Scott syndrome: Clinical update and report of nine novel mutations of the FGD1 gene

When preadipocytes differentiate into adipocytes, several differentiation-linked genes are activated. Lipo-protein lipase (LPL) is one of the first genes induced during this process. To investigate early events in adipocyte development, we have focused on the transcriptional activation of the LPL gene. For this purpose, we have cloned and fused different parts of intragenic and flanking sequences with a chloramphenicol acetyltransferase reporter gene. Transient transfection experiments and DNase I hypersensitivity assays indicate that several positive as well as negative elements contribute to transcriptional regulation of the LPL gene. When reporter gene constructs were stably introduced into preadipocytes, we were able to monitor and compare the activation patterns of different promoter deletion mutants at selected time points representing the process of adipocyte development. We could delimit two cis-regulatory elements important for gradual activation of the ...

1992-10-01

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241

Using n-ZnO-Al as window layer in pilot production of CIGSSe solar modules: Status and trends. Status of CIGSSe pilot scale production at Munich; Einsatz von n-ZnO:Al als Fensterschicht in der Pilotfertigung von CIGSSe-Solarmodulen: Status and Entwicklung. Status der CIGSSe-Pilotfertigung in Muenchen

Mutations in the FGD1 gene have been shown to cause Aarskog-Scott syndrome (AAS), or facio-digito-genital dysplasia (OMIM#305400), an X-linked disorder characterized by distinctive genital and skeletal developmental abnormalities with a broad spectrum of clinical phenotypes. To date, 20 distinct mutations have been reported, but little phenotypic data are available on patients with molecularly confirmed AAS. In the present study, we report on our experience of screening for mutations in the FGD1 gene in a cohort of 60 European patients with a clinically suspected diagnosis of AAS. We identified nine novel mutations in 11 patients (detection rate of 18.33%), including three missense mutations (p.R402Q; p.S558W; p.K748E), four truncating mutations (p.Y530X; p.R656X; c.806delC; c.1620delC), one in-frame deletion (c.2020_2022delGAG) and the first reported splice site mutation (c.1935+3A>C). A recurrent mutation (p.R656X) was detected in three independent families. ...

2010-01-01

242

Regulation of the pT181 encoded tetracycline resistance gene in Straphylococcus aureus

Shell Solar GmbH is working on a variant of the so-called stacked elemental layer process for CIGSSe absorber production. The metal films resp. the selenium are deposited on the Mb electrode at room temperature by DC sputtering (CIG) or evaporation (Se) and then processed into semiconductors by rapid thermal annealing (RTP). Currently, the mean efficiency of the pilot plant is i 12.6 {+-} 0.2 % for non-sealed 30 x 30 cm{sup 2} modules, the key efficiency is 13.0 %. In addition to the 30 x 30 cm{sup 2} pilot line, a small series of 60x90cm2 modules was constructed in December 2004. Here, the mean efficiency was 13.0 percent while the average module power was higher than 60 W. The contribution also describes investigations of ceramically sputtered n-ZnO:Al layers with reduced Al concedntrations of 1 percent and on reactively sputtered n-ZnO.Al layers from the double roller magnetron. (orig.) [German] Bei Shell Solar GmbH wird eine Variante des ...

2005-07-01

243

Variations in humanized and defined culture conditions supporting derivation of new human embryonic stem cell lines

pT181 is a naturally-occurring 4437 basepair (bp) plasmid isolated from Staphylococcus aureus which encodes inducible resistance to tetracycline (Tc). The DNA sequence data has identified three open reading frames (ORFs). The largest ORF B, has been found to be responsible for the Tc resistance phenotype of pT181. Since most Tc resistance systems appear to be regulated by an effector protein and a repressor protein, several Bal 31 deletion mutants of pT181 were constructed and analyzed in an effort to identify the elements involved in Tc resistance. Two transcomplementing groups of mutants were identified within the tet gene. The mechanism of Tc resistance was studied by assaying the accumulation of (7-/sup 3/H) Tc by Tc sensitive cells, and uninduced and induced pT181-containing cells. A sharp decrease in accumulation of the drug after an initial increase was observed in Tc induced pT181-containing cells. In vivo labeling of Bacillus subtilis minicells containing ...

1986-01-01

244

Tissue culture and micropropagation for forest biomass production

The evolution of "humanized" (i.e., free of animal sourced reagents) and ultimately chemically defined culture systems for human embryo stem cell (hESC) isolation and culture is of importance to improving their efficacy and safety in research and therapeutic applications. This can be achieved by integration of a multitude of individual approaches to replace or eliminate specific animal sourced reagents into a single comprehensive protocol. In the present study our objective was to integrate strategies obviating reliance on some of the most poorly defined and path-critical factors associated with hESC derivation, namely the use of animal immune compliment to isolate embryo inner cell mass, and animal sourced serum products and feeder cells to sustain hESC growth and attachment. As a result we report the derivation of six new hESC lines isolated by outgrowth from whole blastocysts on an extracellular matrix substrate of purified human laminin (Ln) with transitional reliance on ...

2006-01-01

245

The ternary silicide ZrPd{sub 3}Si{sub 3}, a stacking variant of the {alpha}-FeSi{sub 2} and Re{sub 3}B structure types

An increase in forest production will be necessary in the future when wood becomes a major renewable source of energy and chemicals along with its traditional role of fibre source. This increase could eventually by achieved be proper selection and breeding of trees. Clonal forestry by vegetative propagation of cuttings is becoming a viable alternative to a seedling-based forestry with many advantages, and cutting could be used to quickly propagate large numbers of clones of control-pollinated seedlings. Most forest trees are propagated sexually and seed orchards were started in the US and Canada in the last 40-50 years for breeding purposes. Forests could ultimately be established with improved seedlings instead of from seed with unknown genetic potential, or by natural regeneration. Micropropagation is the term used to refer to the propagation of plants raised by tissue culture methods rather than from seeds or cuttings. Many clonal plantlets could be regenerated asexually in the ...

1984-09-01

246

The retinoic acid receptor beta (Rarb) region of Mmu14 is associated with prion disease incubation time in mouse.

The ternary zirconium palladium silicide ZrPd{sub 3}Si{sub 3} has been synthesized by arc-melting of the elemental components. It adopts a new structure type and crystallizes in the orthorhombic space group Cmcm with a = 3.8127(4){angstrom}, b = 15.551(1){angstrom}, c = 7.0390(5){angstrom}, and Z = 4 (Pearson symbol oC28). The structure can be regarded as being built up of Re{sub 3}B-type slabs of composition Pd{sub 3}Si alternating with {alpha}-FeSi{sub 2} slabs of composition ZrSi{sub 2}. Notable features include the presence of Si{sub 2} pairs, square pyramidal and tetrahedral coordination of Pd centers by Si atoms, an unusual distorted cubic coordination of the Zr atoms by the Si{sub 2} pairs, and an extensive network of Zr-Zr, Zr-Pd, and Pd-Pd metal-metal bonds. ZrPd{sub 3}Si{sub 3} is weakly metallic with a room-temperature resistivity of 1.7 x 10{sup {minus}3} {Omega} cm. Extended Hueckel band structure calculations confirm the metallic behavior; support the simultaneous ...

1999-11-01

247

The economic limits of in situ leach mining

In neurodegenerative conditions such as Alzheimer's and prion disease it has been shown that host genetic background can have a significant effect on susceptibility. Indeed, human genome-wide association studies (GWAS) have implicated several candidate genes. Understanding such genetic susceptibility is relevant to risks of developing variant CJD (vCJD) in populations exposed to bovine spongiform encephalopathy (BSE) and understanding mechanisms of neurodegeneration. In mice, aspects of prion disease susceptibility can be modelled by examining the incubation period following experimental inoculation. Quantitative trait linkage studies have already identified multiple candidate genes; however, it is also possible to take an individual candidate gene approach. Rarb and Stmn2 were selected as candidates based on the known association with vCJD. Because of the increasing overlap described between prion and Alzheimer's diseases we also chose Clu, Picalm and Cr1, which ...

2010-12-06

248

The CYP2A3 gene product catalyzes coumarin 7-hydroxylation in human liver microsomes

Different technological procedures used for the chemical mining of uranium differ for one thing with a level of the deposit exploitation and for another with the expenditures. It is necessary to find out the highest acceptable value of the costs per unit of production - limit unit costs. This criterion serves both for selection of the minable parts of deposit, and for determination of the exploitation level of the deposit parts. The paper describes a calculation of the costs and their decomposition into the fixed and variable costs. An evaluation of the different variants of the deposit exploitation is based on the variable costs, expressed in the dependence on the parameters of technological procedure. These parameters are either primary, e.g. density of the wells or dosage of reagents, or secondary, e.g. the uranium production. A relation between primary and secondary variables is defined by the technological models. The first economic optimization calculations ...

2002-03-01

249

The CYP2A3 gene product catalyzes coumarin 7-hydroxylation in human liver microsomes

Three cDNAs, designated IIA3, IIA3v, and IIA4, coding for P450s in the CYP2A gene subfamily were isolated from a {lambda}gt11 library prepared from human hepatic mRNA. Only three nucleotide differences and a single amino acid difference, Leu{sup 160}{yields}His, were found between IIA3 and IIA3v, indicating that they are probably allelic variants. IIA4 displayed 94% amino acid similarity with IIA3 and IIA3v. The three cDNAs were inserted into vaccinia virus, and recombinant viruses were used to infect human hepatoma Hep G2 cells. Only IIA3 was able to produce an enzyme that had a reduced CO-bound spectrum with a {lambda}{sub max} at 450 nm. This expressed enzyme was able to carry out coumarin 7-hydroxylation and ethoxycoumarin O-deethylation. cDNA-expressed IIA3v and IIA4 failed to incorporate heme and were enzymatically inactive. Analysis of IIA proteins in human liver microsomes, using antibody against rat IIA2, revealed two proteins of 49 and 50 kDa, the former ...

1990-02-06

250

Tests for the diagnosis of Clostridium difficile infection: the next generation.

Three cDNAs, designated IIA3, IIA3v, and IIA4, coding for P450s in the CYP2A gene subfamily were isolated from a #lambda#gt11 library prepared from human hepatic mRNA. Only three nucleotide differences and a single amino acid difference, Leu"1"6"0#->#His, were found between IIA3 and IIA3v, indicating that they are probably allelic variants. IIA4 displayed 94% amino acid similarity with IIA3 and IIA3v. The three cDNAs were inserted into vaccinia virus, and recombinant viruses were used to infect human hepatoma Hep G2 cells. Only IIA3 was able to produce an enzyme that had a reduced CO-bound spectrum with a #lambda#_m_a_x at 450 nm. This expressed enzyme was able to carry out coumarin 7-hydroxylation and ethoxycoumarin O-deethylation. cDNA-expressed IIA3v and IIA4 failed to incorporate heme and were enzymatically inactive. Analysis of IIA proteins in human liver microsomes, using antibody against rat IIA2, revealed two proteins of 49 and 50 kDa, the former of ...

1990-02-01

251

Strikingly different penetrance of LHON in two Chinese families with primary mutation G11778A is independent of mtDNA haplogroup background and secondary mutation G13708A

Clostridium difficile (C. difficile) causes 25-30% of cases of antibiotic associated diarrhea and most cases of pseudomembranous colitis. Patients presenting with diarrhea after hospitalization for 3 or more days should be tested for C. difficile. There are many options available for testing, each of which has inherent advantages and disadvantages. Most laboratories perform toxin testing using an enzyme immunoassay method. In general these tests have sensitivities ranging from 60 to 70% and specificities of 98%. When using these methods, symptomatic patients with negative tests should be tested by another more sensitive method. Until recently, cell culture cytotoxicity neutralization assays (CCNAs) were considered the gold standard in the U.S. A two-step algorithm using an EIA for glutamate dehydrogenase detection followed by testing positives using CCNA, offered an improved alternative until the availability of molecular assays. Although early studies that compared the GDH assay to ...

2011-03-03

252

Strikingly different penetrance of LHON in two Chinese families with primary mutation G11778A is independent of mtDNA haplogroup background and secondary mutation G13708A

The penetrance of Leber's hereditary optic neuropathy (LHON) in families with primary mitochondrial DNA (mtDNA) mutations is very complex. Matrilineal and nuclear genetic background, as well as environmental factors, have been reported to be involved in different affected pedigrees. Here we describe two large Chinese families that show a striking difference in the penetrance of LHON, in which 53.3% and 15.0% of members were affected (P < 0.02), respectively. Analysis of the complete mtDNA genome of the two families revealed the presence of the primary mutation G11778A and several other variants suggesting the same haplogroup status G2a. The family with higher penetrance contained a previously described secondary mutation G13708A, which presents a polymorphism in normal Chinese samples and does not affect in vivo mitochondrial oxidative metabolism as described in a previous study. Evolutionary analysis failed to indicate any putatively pathogenic ...

2008-08-25

253

Site-specific semisynthetic variant of human hemoglobin

2008-08-25

254

Safe detection of Co-60. New perspectives in contamination and clearance measurements

A single round of Edman degradation was employed to remove the NH_2-terminal valine from isolated #alpha# chains of human hemoglobin. Reconstitution of normal #beta# chains with truncated or substituted #alpha# chains was used to form truncated (des-Val"1-#alpha#1) and substituted ([[1-"1"3C]Gly"1]#alpha#1) tetrameric hemoglobin analogs. Structural homology of the analogs with untreated native hemoglobin was established by using several spectroscopic and physical methods. Functional studies indicate that the reconstituted tetrameric protein containing des-Val"1-#alpha# chains has a higher affinity for oxygen, is less influenced by chloride ions or 2,3-biphosphoglycerate, and shows lower cooperativity than native hemoglobin. These results confirm the key functional role of the #alpha#-chain NH_2 terminus in mediating cooperative oxygen binding across the dimer interface. The NH_2-terminal pK/sub 1/2/ value was determined for the ["1"3C]glycine-substituted analog to be 7.46 +/- 0.09 at ...

255

Roles of the #beta# 146 histidyl residue in the molecular basis of the Bohr Effect of hemoglobin: A proton nuclear magnetic resonance study

Consistent application of a newly developed coincidence technique to determine activation products in the operation and demolition of nuclear facilities results in very new perspectives in contamination monitoring and clearance measurement of plant components. In particular, it is possible to avoid the critical spread of point-source Co-60 contamination, the so-called hot particles. The CCM (Cobalt Coincidence Measurement) technique includes the coincident detection of prompt-prompt gamma cascades with "6"0Co and other activation products ("1"1"0"mAg, "1"2"4Sb, "2"2Na) with low mass-specific limits for unrestricted clearance. Special features of the technique are the short measurement times, excellent signal-to-noise ratio, and focusing in space achieved. Compared to conventional integral gamma-measurements, the signal-to-background ratio can be improved by 1-2 orders of magnitude. The method can be employed especially where conventional integral or spectroscopic methods suffer from ...

256

Polymorphisms in fatty acid metabolism-related genes are associated with colorectal cancer risk

Assessment of the roles of the carboxyl-terminal #beta#146 histidyl residues in the alkaline Bohr effect in human and normal adult hemoglobin by high-resolution proton nuclear magnetic resonance spectroscopy requires assignment of the resonances corresponding to these residues. By a careful spectroscopic study of human normal adult hemoglobin, enzymatically prepared des(His146#beta#)-hemoglobin, and the mutant hemoglobins Cowtown (#beta#146His #-># Leu) and York (#beta#146His #-># Pro), the authors have resolved some of these conflicting results. By a close incremental variation of pH over a wide range in chloride-free 0.1 M N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid buffer, a single resonance has been found to be consistently missing in the proton nuclear magnetic resonance spectra of these hemoglobin variants. The results indicate that the contribution of the #beta#146 histidyl residues is 0.52 H"+/hemoglobin tetramer at pH 7.6, markedly less ...

257

Novel bifunctional natriuretic peptides as potential therapeutics.

Colorectal cancer is the third most common malignant tumor and the fourth-leading cause of cancer death worldwide. The crucial role of fatty acids for a number of important biological processes suggests a more in depth analysis of inter-individual differences in fatty acid metabolizing genes as contributing factor to colon carcinogenesis. We examined the association between genetic variability in 43 fatty acid metabolism-related genes and colorectal risk in 1225 CRC cases and 2032 controls participating in the European Prospective Investigation into Cancer and Nutrition study. 392 single nucleotide polymorphisms were selected using pairwise tagging with an r(2) cutoff of 0.8 and a minor allele frequency of >5%. Conditional logistic regression models were used to estimate odds ratios and corresponding 95% confidence intervals. Haplotype analysis was performed using a generalized linear model framework. On the genotype level, HPGD, PLA2G6, and TRPV3 were associated with higher risk ...

2010-01-01

258

Marine transportation of oil from Timan Pechora and from inland Russian fields

Synthetic atrial natriuretic peptide (carperitide) and B-type natriuretic peptide (BNP; nesiritide) are used to treat congestive heart failure. However, despite beneficial cardiac unloading properties, reductions in renal perfusion pressures limit their clinical effectiveness. Recently, CD-NP, a chimeric peptide composed of C-type natriuretic peptide (CNP) fused to the C-terminal tail of Dendroaspis natriuretic peptide (DNP), was shown to be more glomerular filtration rate-enhancing than BNP in dogs. However, the molecular basis for the increased responsiveness was not determined. Here, we show that the DNP tail has a striking effect on CNP, converting it from a non-agonist to a partial agonist of natriuretic peptide receptor (NPR)-A while maintaining the ability to activate NPR-B. This effect is specific for human receptors because CD-NP was only a slightly better activator of rat NPR-A due to the promiscuous nature of CNP in this species. Interesting, the DNP tail alone had no effect ...

2008-10-21

259

Mapping of the human cone transducin {alpha} subunit (GNAT2) gene to 1p13 and mutation analysis in patients with Stargardt`s disease

As part of The International Northern Sea Route Programme (INSROP), Subprogram III, Trade and Commercial Shipping, a study has been made concerning seaborne export of liquefied petroleum gas (LPG) from the Northern Russia, especially the West Siberian fields in Tyumen. The main purpose of the total project III.0703, part 2 and this study is to evaluate the economic viability of seaborne export from this area to the European region, mainly with the use of a special ice-strengthened LPG vessel, constructed and designed for such seaborne operations. This study concentrates on both seaborne LPG transportation, the demand and supply for seaborne LPG in the world and marine transportation of LPG from the West Siberian fields. Another purpose is to see which regions are potential exporters, importers and buyers of seaborne export of LPG from Tyumen. Currently large quantities of liquefied gas are flared off at the West Siberian fields due to insufficient infrastructure, lack of modern ...

1996-05-01

260

Influence of two changes in the composition of an acrylic bone cement on its handling, thermal, physical, and mechanical properties.

Transducin {alpha} subunits are members of a large family of G-proteins and play an important role in phototransduction in rod and cone photoreceptors. We report the localization of the human cone {alpha} transducin (GNAT2) gene using fluorescence in situ hybridization (FISH) on chromosome 1 in band p13. The recent assignment of a gene for Stargardt`s disease to the same chromosomal region by linkage analysis prompted us to investigate the possible role of GNAT2 in the pathogenesis of this disease. Stargardt`s disease is characterized by degeneration in late childhood or early adulthood of the macula of the retina, a region rich in cones. We screened patients with Stargardt`s disease, with or without peripheral cone involvement as monitored by the full-field ERG, for mutations in this gene. We investigated 66 unrelated patients including 22 with peripheral cone dysfunction for mutations in the coding region of the GNAT2 gene using polymerase chain reaction-single strand conformation ...

1994-09-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14

261

Genomic analysis of the symbiotic marine crenarchaeon, Cenarchaeumsymbiosum

This study is a contribution to the growing body of work on the influence of changes in the composition of an acrylic bone cement on various properties of the curing and cured material. The focus is on one commercially-available acrylic bone cement brand, Surgical Simplex P, and three variants of it and a series of properties, namely, setting time, maximum exotherm temperature, activation energy and frequency factor for the polymerization reaction, diffusion coefficient for the uptake of phosphate buffered saline, at 37 degrees C, ultimate compressive strength (UCS), plane-strain fracture toughness, fatigue life (under fully-reversed tension-compression stress), hardness (H) and elastic modulus (both determined using quasi-static nanoindentation), and the variation of the storage and loss moduli with frequency of the applied force in a dynamic nanoindentation test. It was found that (a) a 68% reduction in the volume of the activator, N,N dimethyl-4-toluidine, ...

2007-05-05

262

Extracellular ATP4- promotes cation fluxes in the J774 mouse macrophage cell line

Crenarchaea are ubiquitous and abundant microbial constituents of soils, sediments, lakes and ocean waters, yet relatively little is known about their fundamental evolutionary, ecological, and physiological properties. To better describe the ubiquitous nonthermophilic Crenarchaea, we analyzed the genome sequence of one representative, the uncultivated sponge symbiont, Cenarchaeum symbiosum. C. symbiosum genotypes coinhabiting the same host partitioned into two dominant populations, corresponding to previously described a- and b-type ribosomal RNA variants. Although synthetic, overlapping a- and b-type ribotypes harbored significant genetic variability. A single tiling path comprising the dominant a-type genotype was assembled, and used to explore the biological properties of C. symbiosum and its planktonic relatives. Out of a total of 2,066 predicted open reading frames, 36% were more highly conserved with other Archaea. The remainder partitioned between bacteria ...

2006-06-24

263

Cost of ownership for military cargo aircraft using a common versus disparate display configuration

Extracellular ATP stimulates transmembrane ion fluxes in the mouse macrophage cell line J774. In the presence of Mg2+, nonhydrolyzable ATP analogs and other purine and pyrimidine nucleotides do not elicit this response, suggesting the presence of a specific receptor for ATP on the macrophage plasma membrane. One candidate for such a receptor is the ecto-ATPase expressed on these cells. We, therefore, investigated the role of this enzyme in ATP-induced /sup 86/Rb+ efflux in J774 cells. The ecto-ATPase had a broad nucleotide specificity and did not hydrolyze extracellular ATP in the absence of divalent cations. /sup 86/Rb+ efflux was not blocked by inhibition of the ecto-ATPase and did not require Ca2+ or Mg2+. In fact, ATP-stimulated /sup 86/Rb+ efflux was inhibited by Mg2+ and correlated with the availability of ATP4- in the medium. In the absence of divalent cations, the slowly hydrolyzable ATP analogs adenosine 5'-(beta, gamma-imido)triphosphate (AMP-PNP) and adenosine ...

1987-03-05

264

Corrosion of stainless steels in 20 to 75% nitric acid. Final report. Korrosion von Chrom-Nickel-(Molybdaen)-Staehlen in unterazeotroper und azeotroper Salpetersaeure. Schlussbericht

A 2009 paper considered possibilities for applying a common display suite to various front-line bubble canopy fighters, whereas further research suggests the cost savings, post Milestone C production/deployment, might not be advantageous. The situation for military cargo and tanker aircraft, may offer a different paradigm. The primary objective of Defense acquisition is to acquire quality products that satisfy user needs with measurable improvements to mission capability and operational support, in a timely manner, and at a fair and reasonable price. DODD 5000.01 specifies that all participants in the acquisition system shall recognize the reality of fiscal constraints, viewing cost as an independent variable. DoD Components must therefore plan programs based on realistic projections of the dollars and manpower likely to be available in future years and also identify the total costs of ownership, as well as the major drivers of total ownership costs. In theory, therefore, this has ...

2010-04-01

265

Computed tomography in the diagnosis of cavum septum pellucidum

A series of stainless austenitic and ferritic materials was exposed for 100 days to boiling nitric acid which contained no corrosion products; the corrosion rates and depths of the grain boundary attack were observed. - Provided the structure is precipitation-free, the following are suitable for long-term exposure; the austenitic steels X 2 CrNi 19 11, X 1 CrNi 25 21, X 1 CrNiMoN 25 22 2 and X 1 NiCrMoCu 31 27 4, the practically Mo-free and Cu-free development steel X 1 NiCr 31 27, and the highly Mo-alloyed variant X 1 NiCrMoCu 31 27 5. - In the case of alloy NiCr21Mo it is advisable to limit the concentration and/or temperature of the nitric acid. - The 'superferrite' X 1 CrNiMoNb 28 4 2, the Japanese steel X 1 CrNiNb 30 2 and the austenitic steels X 2 CrNiMoN 17 13 3 and X 1 CrNiMoN 25 22 2 in the version with high nickel content are unsuitable. The decisive factor affecting the surface-removal rates is the chromium content of the material. The ...

1988-01-01

266

Comparing compressed sequences for faster nucleotide BLAST searches.

The cavum septum pellucidum is an anatomical variants of the septum pellucidum, and lying between two lateral ventricles and this is referred to as the fifth ventricle. Occasionally, posterior extension of the cavum septum pellucidum towards the splenium is seen and this is known as the cavum vergae, or sixth ventricle. The septum is double membrane and occasionally a narrow cavity containing CSF is present between these membrane. The roof of the cavum septum pellucidum is formed by the corpus callosum and its floor by the anterior pillars of the fornix. This cavum outlined with air is occasionally seen in the pneumoencephalography. Brain CT scans of 1000 patients were performed, and the diagnosis could be made on the basis of the characteristic shape, density and location. Other diagnostic procedure are not necessary in these cases. Patients were examined with CT scanner from June-80' to Dec-80', at department of radiology, Maryknoll Hospital, ...

1981-09-15

267

Comparative analysis of budded virus infectivity of Bombyx mandarina and B. mori nucleopolyhedroviruses.

Molecular biologists, geneticists, and other life scientists use the BLAST homology search package as their first step for discovery of information about unknown or poorly annotated genomic sequences. There are two main variants of BLAST: BLASTP for searching protein collections and BLASTN for nucleotide collections. Surprisingly, BLASTN has had very little attention; for example, the algorithms it uses do not follow those described in the 1997 BLAST paper and no exact description has been published. It is important that BLASTN is state-of-the-art: Nucleotide collections such as GenBank dwarf the protein collections in size, they double in size almost yearly, and they take many minutes to search on modern general purpose workstations. This paper proposes significant improvements to the BLASTN algorithms. Each of our schemes is based on compressed bytepacked formats that allow queries and collection sequences to be compared four bases at a time, permitting very fast ...

268

#omega#-Assisted nucleation and growth of #alpha# precipitates in the Ti-5Al-5Mo-5V-3Cr-0.5Fe #beta# titanium alloy

Bombyx mandarina nucleopolyhedrovirus (BomaNPV) is a variant of Bombyx mori nucleopolyhedrovirus (BmNPV). BomaNPV S1 strain has been reported to be significantly less virulent than the BmNPV T3 strain via the oral infection route in B. mori larvae, but other features of S1 including budded virus (BV) infectivity and virus propagation in cultured cells are still unknown. In this study, we compared BV infectivity of S1 and T3 in B. mori larvae and cultured cells. Larval bioassays by intrahemocoelic BV injection revealed that the median lethal dose of S1's BV was approximately three times lower than that of T3. In addition, S1 produced more BVs and occlusion bodies (OBs) in the hemolymph of B. mori larvae compared with T3. Furthermore, we observed that the locomotion was enhanced earlier and the median lethal time was shorter in S1-infected larvae compared with those in T3-infected larvae. Western blot analysis of S1- and T3-infected BmN cells revealed that expression ...

2011-05-22

269

The new WHO classification of brain tumors. Radiological aspects of 4 new tumor entities; Die revidierte WHO-Klassifikation der Hirntumoren. Radiologische Aspekte unter besonderer Beruecksichtigung von vier neuen Tumorentitaeten

This paper discusses the structural and compositional changes at the nanometer scale associated with the nucleation and growth of #alpha# precipitates in the #beta# titanium alloy Ti-5553 (Ti-5Al-5Mo-5 V-3Cr-0.5Fe) with #omega# precipitates acting as heterogeneous nucleation sites. The microstructural evolution in this alloy, during #beta#-solutionizing, quenching and aging type heat-treatments, has been investigated by combining results from scanning electron microscopy, orientation imaging microscopy, transmission electron microscopy, high-resolution TEM and three-dimensional atom probe (3DAP) tomography. Athermal #omega# precipitates form in this alloy on quenching from above the #beta# transus temperature. On isothermal annealing at low temperatures, these #omega# precipitates coarsen to form chemically ordered #omega# precipitates, accompanied by the nucleation of the stable #alpha# phase. Annealing at higher temperatures leads to dissolution of #omega# and further growth of ...

2009-04-01

270

Nature and distribution of mucosal lesions associated with enteropathogenic and enterohemorrhagic Escherichia coli in piglets and the role of plasmid-mediated factors.

Characterisation of the classification of brain tumours authorised by the WHO. Method of appraisal: This classification was revised and published in its second version. In the revision, some tumours were regrouped histogenetically and some tumour variants were added. Radiologically relevant changes of the classification include the differentiation of four new tumour entities that are easily distinguished by MR imaging. These four tumours belong to the group of childhood tumours or tumours occurring in early adulthood and are characterized by a good prognosis after extirpation. Results of appraisal: Central neurocytomas are small-cyst ventricular tumours associated with the foramen of Monroi and show moderate contrast enhancement. Infantile desmoplastic gangliogliomas/astrocytomas commonly consist of a solid tumour portion related to the leptomeninges with pronounced contrast enhancement and a typically very large cyst. Pleomorphic xanthostrocytomas are ...

1997-06-01

271

Medium voltage network planning based on probabilistic reliability evaluation including the insurance system

Bacterial attachment-effacement (att-eff) is emerging as an important virulence characteristic common to both enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC). The contribution of the plasmid-encoded EPEC adherence factor to the production of mucosal lesions and diarrhea was investigated in gnotobiotic piglets. Bacterial att-aff in the intestinal mucosa of piglets infected with plasmid-cured EPEC strain E2348/69 (O127) was indistinguishable from that in piglets infected with the parent strain, but the distribution of lesions was different; it occurred in the small intestines of 6 of 7 piglets infected with the parent strain compared with only 2 of 11 (P = 0.006) infected with the plasmid-cured strain. Plasmid-encoded factors in EPEC and EHEC strains did not appear to contribute to bacterial competition with normal gut microflora. Of 13 strains belonging to five EPEC serogroups, O55, O142, O26, O119, and O111, 3 fulfilled the criteria for EHEC (2 O26 and 1 ...

1989-04-01

272

Cutaneous and subcutaneous Ewing's sarcoma: an indolent disease

In comparison to deterministic criteria, probabilistic reliability indices like interruption frequency and unavailability give a more differentiated insight into reliability in an electrical energy network. One possibility for network planning based on these indices is the definition of limits for customer nodes, another is the monetarisation of reliability in terms of interruption costs. Because of numerous disadvantages, interruption cost methods could not up to now win broad recognition in network planning in Western Europe. A more recent method for the monetarisation of reliability circumventing most of these disadvantages is the insurance system. In this system the customer selects a tariff class in an insurance system, and the utility reimburses its customers in the case of an interruption. In its first section, the paper describes a method for the flexible and realistic optimisation of MV networks. This method based on Genetic optimisation is able to integrate probabilistic ...

1997-07-01

273

ZZ KAFAX-F22, 80 and 24 Groups Cross-Section Library in MATXS Format Based on JEF-2.2 for Fast Reactors

Purpose: The occurrence of extraosseous Ewing's sarcoma (ES) in deep soft tissues has been well described, but cases in which this tumor occurs in a primary cutaneous or subcutaneous site have rarely been reported. The superficial variant may be less aggressive than are the more common bony and deep soft tissue counterparts with an apparently favorable outcome. A retrospective review of patients with cutaneous or subcutaneous ES was conducted to analyze outcome and patterns of failure. Methods and Materials: Between July 1985 and March 1997, 14 patients with cutaneous or subcutaneous ES were treated at St. Jude Children's Research Hospital. The median age at presentation was 16 years (range 7-21 years). Anatomic locations included trunk and pelvis (7), upper or lower extremity (4), and head and neck (3). The median size of the lesion was 3 cm (range, 1-12 cm). Thirteen had definitive surgical resections, and one had biopsy of the mass at the time of referral. They ...

2000-01-15

274

The development of the high-temperature reactor. In memoriam Prof. Dr. rer.nat. Dr.-Ing. e.h. Rudolf Schulten; Die Entwicklung des Hochtemperaturreaktors. Zum Tode von Prof. Dr. rer.nat. Dr.-Ing. e.h. Rudolf Schulten

1 - Description: Format: MATXS. Number of groups: 80 neutron-, 24 photon-groups. 97 Nuclides: 1-H-1, 1-H-2, 2-He-3, 2-He-4, 3-Li-6, 3-Li-7, 4-Be-9, 5-B-10, 5-B-11, 6-C- nat., 7-N-14, 7-N-15, 8-O-16, 9-F-19, 11-Na-23, 12-Mg-nat., 13-Al-27, 14-Si-nat., 15-P-31, 17-Cl-nat., 18-Ar-40, 19-K-nat., 20-Ca-nat., 22-Ti-nat., 23-V-nat., 24-Cr-50, 24-Cr-52, 24-Cr-53, 24-Cr-54, 25-Mn-25, 26-Fe-54, 26-Fe-56, 26-Fe-57, 26-Fe-58, 27-Co-59, 28-Ni-58, 28-Ni-60, 28-Ni-61, 28-Ni-62, 28-Ni-64, 29-Cu-nat., 31-Ga-nat., 39-Y-89, 40-Zr-nat., 41-Nb-93, 42-Mo-nat., 47-Ag-107, 47-Ag-109, 48-Cd-nat., 50-Sn-nat., 63-Eu-151, 63-Eu-153, 64-Gd-152, 64-Gd-154, 64-Gd-155, 64-Gd-156, 64-Gd-157, 64-Gd-158, 64-Gd-160, 73-Ta-181, 74-W-182, 74-W-183, 74-W-184, 74-W-186, 75-Re-185, 75-Re-187, 79-Au-197, 82-Pb-nat., 83-Bi-209, 90-Th-232, 91-Pa-233, 92-U-232, 92-U-233, 92-U-234, 92-U-235, 92-U-236, 92-U-237, 92-U-238, 93-Np-237, 93-Np-238, 94-Pu-238, 94-Pu-239, 94-Pu-240, 94-Pu-241, 94-Pu-242, 95-Am-241, 95-Am-242, 95-Am-242m, ...

275

MRI in preoperative evaluation of chronic paranasal sinusitis - a comparison with CT; MRT in der praeoperativen Diagnostik der chronischen Sinusitis im Vergleich mit der CT

1996-08-01

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