vacunales del virus: Topics by WorldWideScience.org

Review of Vaccinia Virus and Baculovirus Viability Versus ...

... 2010-10-01 true Information Technology Virus Security. 2452...Clauses 2452.239-71 Information Technology Virus Security. As prescribed...the following clause: Information Technology Virus Security (FEB...

2010-10-01

8

Podcast: Viruses and Cooking

... hazards. One study targeted the effects of common household disinfectants on virus inactivation (Butcher and Ulaeto, 2005). ...

2008-03-01

9

Effects of cyclosporin A treatment on the pathogenesis of avian leukosis virus subgroup J infection in broiler chickens with Marek's disease virus exposure.

The Podcast: Viruses and Cooking is a segment of the Web Seminar: Teaching Science with Food Safety , April 27 2010. The podcast is 14 minutes 11

1900-01-01

10

Simultaneous Detection of Multiplex-Amplified Human Immunodeficiency Virus Type 1 RNA, Hepatitis C Virus RNA, and Hepatitis B Virus DNA Using a Flow Cytometer Microsphere-Based Hybridization Assay

Full Text Available

2003-12-01

11

RefLexiones sobre la inmensidad del universo.La materia vista desde la Grecia antigua hasta la era m

The feasibility of performing a multiplex assay for the detection of human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) RNAs and hepatitis B virus (HBV) DNA is demonstrated. This...Full Text Available

2000-03-01

12

Programma PLOT per l'esecuzione ed il confronto di grafici con l'RGL-library del VAX-11/780 Digital

RefLexiones sobre la inmensidad del universo.La materia vista desde la Grecia antigua hasta la era m

2009-01-01

13

Understanding and managing the stressed Mexico-USA transboundary Hueco bolson aquifer in the El Paso del Norte region as a complex system

Programma PLOT per l'esecuzione ed il confronto di grafici con l'RGL-library del VAX-11/780 Digital

1985-01-01

14

Influence of chlorides in the atmospheric corrosion of the Gulf of Mexico; Influencia de los cloruros en la corrosion atmosferica del Golfo de Mexico

Not Available

2005-10-01

15

La falta de financiacion publica hipoteca el futuro del acelerador de protones valenciano. La Universidad de Valencia tiene avanzado el diseno de este dispositivo de alta tecnologia

No abstract prepared.

1999-07-01

16

CERN Multimedia

"La Universidad de Valencia tiene avanzado el diseno de un acelerador lineal de protones, cuya culminacion depende del compromiso del Consell en sufragar la plantilla que necesitaria el centro para estar operativo" (1 page).

2002-01-01

17

Effect of radiation on certain animal viruses in liquid swine manure

Light-dependent regulation of DEL1 is determined by the antagonistic action of E2Fb and E2Fc.

The virucidal effect of _6_0Co gamma radiation was studied in cell culture medium and in liquid swine manure involving the most important porcine viruses that can be spread by liquid manure. The radiation doses (20 kGy and 30 kGy) were determined in preliminary experiments employing a porcine enterovirus from the serogroup 1 (Teschen group). In the main experiment, the following viruses were employed: swine vesicular disease (SVD) virus, type C foot-and-mouth disease (FMD) virus, a field strain of Aujeszky's disease (AD) virus, transmissible gastroenteritis (TGE) virus, as well as bovine viral diarrhoea (BVD) virus. The latter strain served as a model for hog cholera virus. The results of the experiments indicate that safe disinfection of the virus infected liquid swine manure by ionizing radiation ...

1983-01-01

18

Spontaneous mutations affecting the host range of the B77 strain of avian sarcoma virus involve type-specific changes in the virion envelope antigen.

Endoreduplication represents a cell cycle variant during which multiple rounds of DNA replication occur without subsequent chromosome separation and cytokinesis, resulting into a cellular increase of the DNA content. Although the DNA ploidy level of cells is controlled by external stimuli such as light, currently limited knowledge is available on how environmental signals regulate the endoreduplication cycle at the molecular level. Previously, we have demonstrated that the conversion from the mitotic cell cycle into an endoreduplication cycle is mediated by the atypical E2F transcription factor DEL1 that operates as a repressor of endocycle onset. Here, we identified DEL1 as a transcriptional target of the classical E2Fb and E2Fc transcription factors that antagonistically control DEL1 transcript levels through competition for a single E2F cis-acting binding site. Correspondingly with the reported opposite effect of light ...

2011-09-01

19

Weak Association between SEN Virus Viremia and Liver Disease

Previously it was shown that the host-range gene of the Bratislava strain of avain sarcoma virus (B77 virus) spontaneously mutates with a very high rate. The wild-type B77 virus called B77 virus-II,...Full Text Available

1977-01-01

20

Variant influenza virus hemagglutinin that induces fusion at elevated pH.

Recently, a novel DNA virus designated SEN virus (SEN-V), which is thought to be related to posttransfusion hepatitis, was discovered. The aim of the present study was to clarify the relationship between...Full Text Available

2002-09-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

21

Two Proline-Rich Nuclear Localization Signals in the Amino- and Carboxyl-Terminal Regions of the Borna Disease Virus Phosphoprotein

The hemagglutinin (HA) glycoprotein of influenza virus performs two critical roles during infection: it binds virus to cell surface sialic acids, and under mildly acidic conditions it induces fusion...Full Text Available

1986-02-01

22

Persistence of genetic variants of the arctic fox strain of Rabies virus in southern Ontario

Borna disease virus (BDV) uses a unique strategy of replication and transcription which takes place in the nucleus, unlike other known, nonsegmented, negative-stranded RNA viruses of animal origin....Full Text Available

1998-12-01

23

Persistence in herpes simplex virus infections.

AbstractGenetic-variant analysis of rabies viruses provides the most sensitive epidemiologic tool for following the spread and persistence of these viruses in their wildlife hosts. Since...Full Text Available

2006-01-01

24

Lethal Silver-Haired Bat Rabies Virus Infection Can Be Prevented by Opening the Blood-Brain Barrier?

Diseases of man caused by the virus of herpes simplex fall into two broad categories. The primary disease occurs only once in any individual's life and is caused by transmission of virus from an already...Full Text Available

1978-09-01

25

Interaction between Bluetongue virus outer capsid protein VP2 and vimentin is necessary for virus egress

Silver-haired bat rabies virus (SHBRV) infection induces a strong virus-specific immune response in the periphery of the host, but death is common due to the failure to open the blood-brain barrier...Full Text Available

2007-08-01

26

Expression of SV40 virus large T antigen by recombinant adenoviruses activates proliferation of corneal endothelium in vitro.

BackgroundThe VP2 outer capsid protein Bluetongue Virus (BTV) is responsible for receptor binding, haemagglutination and eliciting host-specific immunity. However, the assembly of...Full Text Available

27

Dengue virus protein recognition by virus-specific murine CD8+ cytotoxic T lymphocytes.

Infection with the Ad5-SVR4 virus was used to introduce the large T antigen encoding region of the SV40 virus into bovine and human corneal endothelial cells. Expression of large T antigen occurred...Full Text Available

1993-04-01

28

A duplex real-time RT-PCR assay for the detection of California serogroup and Cache Valley viruses

The identification of the protein targets for dengue virus-specific T lymphocytes may be useful for planning the development of subunit vaccines against dengue. We studied the recognition by murine...Full Text Available

1993-02-01

29

A Cellular J-Domain Protein Modulates Polyprotein Processing and Cytopathogenicity of a Pestivirus

A duplex TaqMan real-time RT-PCR assay was developed for the detection of California (CAL) serogroup viruses and Cache Valley virus (CVV), for use in human surveillance. The targets selected...Full Text Available

2009-10-01

30

Viruses and thyroiditis: an update

Pestiviruses are positive-strand RNA viruses closely related to human hepatitis C virus. Gene expression of these viruses occurs via translation of a polyprotein, which is further processed by cellular...Full Text Available

2001-10-01

31

Viral infections are frequently cited as a major environmental factor involved in subacute thyroiditis and autoimmune thyroid diseases This review examines the data related to the role of viruses in...Full Text Available

32

CDC - XMRV Home - XMRV

MRI with SPIR sequences of optic nerve lesions; Utilita' delle sequenze 'Selective Partial Inversion Recover' (SPIR) nelle mallattie del nervo ottico

For more information about this message, please visit this page: About CDC.gov. XMRV (Xenotropic Murine Leukemia Virus-related Virus) XMRV Home Questions & Answers Updates...

2011-10-16

33

Antarctica and the Mediterranean high mountains. - NASA

To evaluate the yield of SPIR sequences with fat suppression in the diagnosis of optic nerve lesions. T1 and T2 weighted fat suppressed SPIR imaging of the optic nerve improves anatomical definition, lesion detection and characterization in optic nerve conditions. [Italian] L'articolo analizza i vantaggi ricavabili dall'utilizzazione delle sequenze SPIR nella documentazione del decorso del nervo ottico e delle sue alterazioni nei pazienti con sospetto clinico e strumentale di malattia retrobulbare. L'utilizzo delle sequenze SPIR consente ottimale visualizzazione del nervo ottico e delle sue eventuali alterazioni senza gli artefatti che limitavano la qualita' delle immagini nelle prime sequenze RM con soppressione del segnale del grasso.

1999-04-01

34

A study of the incubation times of a new solid phase system for the assay of specific IgE with RAST

como un factor determinante del maximo indice de fotosintesis y, consecuentemente potencial de produccion primaria. Comparaciones entre especies ...

35

Xenotropic murine leukaemia virus-related virus (XMRV) does not cause chronic fatigue.

(1985). Italy Pisati, P. Aprile, C. Fondazione Clinica del Lavoro, Pavia (Italy).

36

The antiviral action of common household disinfectants and antiseptics against murine hepatitis virus, a potential surrogate for SARS coronavirus.

The xenotropic murine leukaemia virus-related virus (XMRV), a gammaretrovirus, was discovered in prostate cancer tumours by Virochip technology in 2006. It was subsequently detected in chronic fatigue patients in 2009. The association between XMRV and chronic fatigue has proved to be controversial. No study has confirmed these findings and many have refuted them. Here, we present the evidence for our contention that XMRV is not a human pathogen. PMID:21978843

2011-10-01

37

Studies of inactivation, retardation and accumulation of viruses in porous media by a combination of dye labeled and native bacteriophage probes

PMID:19692148

2009-08-18

38

SURVIVAL OF VIRUSES OF THE ENTEROVIRUS GROUP (POLIOMYELITIS, ECHO, COXSACKIE) IN SOIL AND ON VEGETABLES.

Not Available

2011-06-01

39

Nanomachines: How Viruses Work, and How We Can Stop Them

PMID:14238948

1964-01-01

40

Prenatal Care Visits

Medline Plus

... a test for HIV, the virus that causes AIDS. Do a Pap smear to check for cervical ...

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

41

ScienceCinema

...the development of biomedicine ...

42

METHOD FOR DETECTING PATHOGENIC VIRUS OF CRUSTACEAN BY LAMP METHOD AND DETECTION REAGENT KIT

J-STORE (Japan)

Full Text Available

2009-08-21

43

Large T1 oligonucleotides of Moloney leukemia virus missing in an env gene recombinant, HIX, are present on an intracellular 21S Moloney viral RNA species.

In Vitro Antiviral Activity of Aqueous Extracts from Korean Medicinal Plants Against Influenza Virus Type A

HIX, a recombinant derived from Moloney leukemia virus, has an envelope glycoprotein different from that of the Moloney virus. HIX and Moloney viruses share the majority of the large T1 oligonucleotides...Full Text Available

1978-06-01

44

Identification of a New Potyvirus, Keunjorong mosaic virus in Cynanchum wilfordii and C. auriculatum

Full Text Available

2005-10-01

45

Genetic Characterization of Encephalomyocarditis Virus Isolated from Aborted Swine Fetus in Korea

Full Text Available

2010-12-01

46

ELECTRON MICROSCOPE STUDIES INTO THE ...

Full Text Available

2006-10-01

47

A recombinant Yellow Fever 17D vaccine expressing Lassa virus glycoproteins

ELECTRON MICROSCOPE STUDIES INTO THE MORPHOLOGY AND LOCALIZATION OF OMSK HEMORRHAGIC FEVER VIRUS IN INFECTED ...

1966-08-01

48

Thermoactivation of viruses by microwaves

The Yellow Fever Vaccine 17D (YFV17D) has been used as a vector for the Lassa virus glycoprotein precursor (LASV-GPC) resulting in construction of YFV17D/LASV-GPC recombinant virus. The virus...Full Text Available

2006-02-20

49

Coal liquefaction provides increased security of oil supply. La licuefaccion del carbon ofrece una mayor independencia del suministro petrolero

Eight different viruses, suspended in drinking water, were examined for their ability to be inactivated by microwaves from a microwave oven. Up to a virus content of 10/sup 5/ TCID/sub 50//ml inactivation was successful within a few minutes of microwave treatment and occurred in parallel to the heat stability of the viruses. Evidence for direct effects of microwaves on viruses could not be detected. 7 of the viruses studied were inactivated rapidly when temperatures of 50 to 65/sup 0/C under microwave treatment were reached in the flowing water, while a bovine parvovirus was only inactivated by temperatures above 90/sup 0/C. The advantages of a thermal virus-decontamination of fluids and material by microwaves are discussed.

1981-01-01

50

The UK's reserves of crude oil could last another 300 years. This is the result it is hoped to obtain from tests currently being carried out in British Coal's liquefaction project of which the objective is to produce a high quality liquid fuel from coal. 1 fig.

1991-07-01

51

2006d.rms - NASA

Use of chest sonography in acute-care radiology

... $84JAN07X 133 147.654 ps 56 fs/s RMS_DEL: $95NOV21DX 607 144.552 ps 208 ...... $86MAR09XI 378 34.285 ps 77 fs/s RMS_DEL: $95MAR23XA 1628 34.270 ps 223 ...

52

British Library Electronic Table of Contents (United Kingdom)

Sommario La patologia polmonare acuta rappresenta una sfida quotidiana per il Radiologo d'Urgenza, al quale e richiesto di porre una diagnosi sulla base di un radiogramma del torace, eseguito spesso in condizioni tecniche precarie. La Tomografia Computerizzata (TC), pur essendo uno strumento decisivo per ovviare alla scarsa accuratezza del radiogramma del torace, non sempre e eseguibile in questi pazienti che necessitano di assistenza continua. Recentemente e stato proposto l'impiego dell'ecografia toracica per lo studio della patologia acuta. L'esame e eseguito rapidamente al letto del paziente e non necessita di apparecchiature particolari. In 168 pazienti con patologia toracica acuta e stato eseguito uno studio ecografico del torace a completamento dell'esame radiologico. Sono stati val...

2008-01-01

53

Antigenic characterization of phocine distemper virus causing mass mortality in 2002 and its relationship to other morbilliviruses

British Library Electronic Table of Contents (United Kingdom)

Summary The antigenic relationship between the phocine distemper virus (PDV) strain causing the epidemic in 2002 and the PDV strain of 1988, canine distemper virus from two dogs and one marten, and one measles virus strain was investigated in vivo and in vitro using monospecific polyclonal and monoclonal antibodies directed against five different proteins of canine or phocine distemper virus (N, P, M, F, H). Epitopic mapping revealed no difference between the PDV strains causing the epidemics in 1988 or 2002. However, the use of these antibodies allowed discrimination between different morbilliviruses including a vaccine strain of canine distemper virus. The major differences among the investigated morbilliviruses were found in the H protein.

2007-01-01

54

Adoptive immunotherapy with virus-specific T cells

British Library Electronic Table of Contents (United Kingdom)

Viral infections are still common causes of morbidity and mortality in immunosuppressed patients after allogeneic hematopoietic stem cell transplantation. Infections caused by virus such as cytomegalovirus, adenovirus and Epstein-Barr virus are well-known. In addition, several other viruses such as polyomavirus and human herpesvirus 6 have been recently reported to be causes of significant complications. As the delay in recovery of virus-specific cellular immune response after transplant is associated with viral reactivation and viral disease, adoptive immunotherapy to restore virus-specific cellular immunity is an attractive option. Recent clinical trials showed the safety and effectiveness of adoptive immunotherapy against viral diseases. In this review, we summarize the current status o...

2011-01-01

55

Contrast material extravasation following upper abdominal trauma. Detection with helical Computerized Tomography; Stravaso del mezzo di contrasto nei traumi dell'addome superiore. Riconoscimento con Tomografia Computerizzata spirale

Two separate domains within vesicular stomatitis virus phosphoprotein support transcription when added in trans.

Active contrast material extravasation can be recognized with conventional CT scanners, though it has been considered a rare finding. Helical CT seems to increase the detection rate and especially to boost the radiologist's confidence in this diagnosis. Though active bleeding is defined in severely-injured subjects requiring urgent intervention and may be associated with findings of hypovolaemic shock, it should not be considered itself as a negative prognostic factor. Contrast extravasation is due to ongoing hemorrhage and its detection is critical for urged treatment. Accurate anatomical location permits to choose surgical management or transcatheter embolization and thus decreases time consumption for precis e bleeding site identification. [Italian] Lo stravaso attivo del mdc e' rilevabile anche con appropriate apparecchiature TC tradizionali, sebbene sia stato considerato raro. La TC sprirale sembra aumentare la frequanza ...

1999-03-01

56

Transcriptional mapping of the 3' end of the bovine syncytial virus genome.

The structural phosphoprotein NS of vesicular stomatitis virus, in association with the virion-associated RNA polymerase L protein, transcribes the genome ribonucleoprotein template in vitro. It contains...Full Text Available

1987-12-01

57

Transcriptional Organization of the Avian Adenovirus CELO

The bovine syncytial virus, a member of the retroviral subfamily Spumavirinae, causes a persistent, asymptomatic infection in cattle. Nucleotide sequence analysis of the viral genome revealed two overlapping...Full Text Available

1994-02-01

58

Theoretical Design of a Gene Therapy To Prevent AIDS but Not Human Immunodeficiency Virus Type 1 Infection

A detailed map of the transcriptional organization of the CELO virus genome was produced. Recent computer analysis of CELO virus has indicated the presence of 38 putative open reading frames (ORFs)....Full Text Available

1998-11-01

59

The Viral Replication Complex Is Associated with the Virulence of Newcastle Disease Virus?

Recent reports confirm that, due to the presence of long-lived, latently infected cell populations, eradication of human immunodeficiency virus type 1 (HIV-1) from infected patients by using antiretroviral...Full Text Available

2003-09-01

60

The Influence of Abacavir and Other Antiretroviral Agents on Virologic Response to Hepatitis C Virus Therapy Among Antiretroviral-Treated HIV-Infected Patients

Virulent strains of Newcastle disease virus ([NDV] also known as avian paramyxovirus type 1) can be discriminated from low-virulence strains by the presence of multiple basic amino acid residues at...Full Text Available

2010-10-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

61

Tat-Neutralizing Antibodies in Vaccinated Macaques

BackgroundIt remains unclear if certain antiretroviral medications, particularly abacavir, compromise response to hepatitis C virus (HCV) therapy. Such data can inform...Full Text Available

2010-01-01

62

Structural analysis of a hepatitis B virus genome integrated into chromosome 17p of a human hepatocellular carcinoma.

The human immunodeficiency virus Tat protein is essential for virus replication and is a candidate vaccine antigen. Macaques immunized with Tat or chemically modified Tat toxoid having the same clade...Full Text Available

2003-03-01

63

Stability of Plasma Human Immunodeficiency Virus Load in VACUTAINER PPT Plasma Preparation Tubes during Overnight Shipment

Hepatitis B virus (HBV) is clearly a factor in the development of hepatocellular carcinoma, but its mechanism of action remains obscure. One possibility is that the HBV integration event alters the...Full Text Available

1988-11-01

64

Specific Residues of the Influenza A Virus Hemagglutinin Viral RNA Are Important for Efficient Packaging into Budding Virions?

VACUTAINER PPT plasma preparation tubes were evaluated to determine the effects of various handling and shipping conditions on plasma human immunodeficiency virus (HIV) load determinations. Plasmas...Full Text Available

2000-01-01

65

Selective Degradation of Newly Synthesized Nonmessenger Simian Virus 40 Transcripts

A final step in the influenza virus replication cycle is the assembly of the viral structural proteins and the packaging of the eight segments of viral RNA (vRNA) into a fully infectious virion. The...Full Text Available

2007-09-01

66

Ross River Virus Transmission, Infection, and Disease: a Cross-Disciplinary Review

By pretreating simian virus 40-infected BSC-1 cells with glucosamine, [³H]uridine labeling of both cellular and viral RNA can be halted instantaneously by addition of cold uridine. We have...Full Text Available

1978-11-01

67

Retrograde neuronal tracing with a deletion-mutant rabies virus

Ross River virus (RRV) is a fascinating, important arbovirus that is endemic and enzootic in Australia and Papua New Guinea and was epidemic in the South Pacific in 1979 and 1980. Infection with RRV...Full Text Available

2001-10-01

68

Recovery of Pathogenic Measles Virus from Cloned cDNA

We have constructed a deletion-mutant rabies virus encoding EGFP and find it to be an excellent tool for studying detailed morphology and physiology of neurons projecting to injection sites...Full Text Available

2007-01-01

69

Quantitation of Antibody to Non-Hemagglutinating Viruses by Single Radial Hemolysis: Serological Test for Human Coronaviruses

Reverse genetics technology so far established for measles virus (MeV) is based on the Edmonston strain, which was isolated several decades ago, has been passaged in nonlymphoid cell lines, and is no...Full Text Available

2000-07-01

70

Purification and translation of murine mammary tumor virus mRNA's.

A single radial hemolysis test was developed for quantitation of specific antibody to non-hemagglutinating viruses. With the human coronaviruses as models, this test utilizes the binding properties...Full Text Available

1977-06-01

71

Production of Infectious Genotype 1b Virus Particles in Cell Culture and Impairment by Replication Enhancing Mutations

We have studied the functions of the intracellular RNAs of mouse mammary tumor virus (MMTV) by purification and translation in vitro. Two major size classes of MMTV RNA, 35S and 24S RNA, were isolated...Full Text Available

1981-07-01

72

Phylogenetic and Evolutionary Relationships among Yellow Fever Virus Isolates in Africa

With the advent of subgenomic hepatitis C virus (HCV) replicons, studies of the intracellular steps of the viral replication cycle became possible. These RNAs are capable of self-amplification in cultured...Full Text Available

2009-06-01

73

Patient-Care Practices Associated With an Increased Prevalence Of Hepatitis C Virus Infection among Chronic Hemodialysis Patients

Previous studies with a limited number of strains have indicated that there are two genotypes of yellow fever (YF) virus in Africa, one in west Africa and the other in east and central Africa. We have...Full Text Available

2001-08-01

74

Pathogenicity of molecularly cloned bovine leukemia virus.

ObjectiveTo identify patient-care practices related to an increased prevalence of hepatitis C virus (HCV) infection among chronic hemodialysis patients.Full Text Available

2011-05-01

75

Palindromic Sequence Plays a Critical Role in Human Foamy Virus Dimerization

To delineate the mechanisms of bovine leukemia virus (BLV) pathogenesis, four full-length BLV clones, 1, 8, 9, and 13, derived from the transformed cell line FLK-BLV and a clone construct, pBLV913,...Full Text Available

1993-12-01

76

Overexpression of human virus surface glycoprotein precursors induces cytosolic unfolded protein response in Saccharomyces cerevisiae

The retroviral RNA genome is dimeric, consisting of two identical strands of RNA linked near their 5′ ends by a dimer linkage structure. Previously it was shown that human foamy virus (HFV)...Full Text Available

2001-04-01

77

New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice.

BackgroundThe expression of human virus surface proteins, as well as other mammalian glycoproteins, is much more efficient in cells of higher eukaryotes rather than yeasts. The limitations...Full Text Available

78

Natural Variation of Drug Susceptibility in Wild-Type Human Immunodeficiency Virus Type 1

A new strain of mouse hepatitis virus (MHV) was isolated from pooled gut suspensions from an epizootic of lethal enteritis in newborn mice. Negative-contrast electron microscopy showed an abundance...Full Text Available

1979-05-01

79

Mutational Analysis of cis-Acting RNA Signals in Segment 7 of Influenza A Virus?

Wild-type viruses from the ViroLogic phenotype-genotype database were evaluated to determine the upper confidence limit of the drug susceptibility distributions, or “biological cutoffs,”...Full Text Available

2004-02-01

80

Multiple integration sites for Moloney murine leukemia virus in productively infected mouse fibroblasts.

The genomic viral RNA (vRNA) segments of influenza A virus contain specific packaging signals at their termini that overlap the coding regions. To further characterize cis-acting signals...Full Text Available

2008-12-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

81

Molecular characterization of hepatitis A virus isolates from environmental and clinical samples in Greece

The integration sites for viral DNA in cells infected with Moloney murine leukemia virus (M-MuLV) were studied by restriction endonuclease cleavage of cellular DNA followed by electrophoresis in agarose...Full Text Available

1979-06-01

82

Molecular characterization of a Chinese variant of the Flury-LEP strain

BackgroundHepatitis A virus (HAV) strains detected in environmental and clinical samples were analysed to characterize the genotypes of HAV circulating in Greece. Fifty (50) sewage...Full Text Available

83

M protein (M1) of influenza virus: antigenic analysis and intracellular localization with monoclonal antibodies.

The entire genome of rabies virus vaccine strain Flury-LEP-C, a Chinese variant of the rabies virus vaccine strain Flury-LEP, was sequenced. The overall length of the genome of Flury-LEP-C strain was...Full Text Available

84

Isolation of caprine arthritis encephalitis virus from goats in Mexico.

A panel of 16 monoclonal antibodies recognizing M protein (M1) of influenza virus was generated. Competition analyses resulted in localization of 14 monoclonal antibodies to three antigenic sites. Three...Full Text Available

1989-09-01

85

Intracellular ribonucleoprotein complexes of visna virus are infectious.

A lentivirus was isolated from 2 goats in Mexico that were seropositive to caprine arthritis encephalitis virus (CAEV) by the agar gel immunodiffusion (AGID) test. The lentivirus was identified as CAEV...Full Text Available

1999-07-01

86

Infection dynamics and clinical manifestations following experimental inoculation of gilts at 90 days of gestation with a low dose of porcine reproductive and respiratory syndrome virus

Sheep choroid plexus cells infected with visna virus produce intracytoplasmic viral ribonucleoprotein complexes with sedimentation values of 120S to 200S and buoyant densities of 1.29 to 1.32 g/cm3....Full Text Available

1982-06-01

87

In vitro dimerization of human immunodeficiency virus type 1 (HIV-1) spliced RNAs

Understanding the dynamics of porcine reproductive and respiratory syndrome virus (PRRSV) vertical transmission is important to enhance the accuracy of monitoring protocols for endemically infected...Full Text Available

2009-10-01

88

In vitro activities of purified visna virus integrase.

The human immunodeficiency virus type 1 (HIV-1) packages its genomic RNA as a dimer of homologous RNA molecules that has to be selected among a multitude of cellular and viral RNAs. Interestingly, spliced...Full Text Available

2007-12-01

89

Identification of a Central DNA Flap in Feline Immunodeficiency Virus

Although integration generally is considered a critical step in the retrovirus life cycle, it has been reported that visna virus, which causes degenerative neurologic disease in sheep, can productively...Full Text Available

1994-06-01

90

Identification of Host Proteins Associated with Retroviral Vector Particles by Proteomic Analysis of Highly Purified Vector Preparations?

A duplication of the polypurine tract (PPT) at the center of the human immunodeficiency virus type 1 (HIV-1) genome (the cPPT) has been shown to prime a separate plus-strand initiation and to result...Full Text Available

2001-10-01

91

Human Immunodeficiency Virus-1 Inhibition of Immunoamphisomes in Dendritic Cells Impairs Early Innate and Adaptive Immune Responses

The Moloney murine leukemia virus (MMLV) belongs to the Retroviridae family of enveloped viruses, which is known to acquire minute amounts of host cellular proteins both on the surface...Full Text Available

2008-02-01

92

Host-Encoded Reporters for the Detection and Purification of Multiple Enveloped Viruses

SUMMARYDendritic cells (DCs) in mucosal surfaces are early targets for human immunodeficiency virus-1 (HIV-1). DCs mount rapid and robust immune responses upon pathogen encounter....Full Text Available

2010-05-28

93

Horizontal Transmissible Protection against Myxomatosis and Rabbit Hemorrhagic Disease by Using a Recombinant Myxoma Virus

The identification of host cell factors for virus replication holds great promise for the development of new anti-viral therapies. Recently, high-throughput screening methods have emerged as...Full Text Available

2010-08-01

94

Hepatitis B virus infection and primary hepatocellular carcinoma.

We have developed a new strategy for immunization of wild rabbit populations against myxomatosis and rabbit hemorrhagic disease (RHD) that uses recombinant viruses based on a naturally attenuated field...Full Text Available

2000-02-01

95

Hepatitis B virus infection among different sex and age groups in Pakistani Punjab

For many years, epidemiological studies have demonstrated a strong link between chronic hepatitis B virus (HBV) infection and the development of primary hepatocellular carcinoma (PHC). Other hepatocarcinogens...Full Text Available

1992-07-01

96

Glycyrrhizin as antiviral agent against Hepatitis C Virus

BackgroundHepatitis B virus (HBV) infection is a serious health problem in the developing countries including Pakistan. Various risk factors are responsible for the spread of this...Full Text Available

97

Genomic but Not Antigenomic Hepatitis Delta Virus RNA Is Preferentially Exported from the Nucleus Immediately after Synthesis and Processing

BackgroundHepatitis C virus is a major cause of chronic liver diseases which can lead to permanent liver damage, hepatocellular carcinoma and death. The presently available treatment...Full Text Available

98

Genetic heterogeneity in human T-cell leukemia/lymphoma virus type II.

Hepatitis delta virus (HDV) contains a viroid-like circular RNA that replicates via a double rolling circle replication mechanism. It is generally assumed that HDV RNA is synthesized and remains exclusively...Full Text Available

2002-04-01

99

Expression and purification of a functional human hepatitis B virus polymerase

DNA from the peripheral blood mononuclear cells of 17 different individuals infected with human T-cell lymphoma/leukemia virus type II (HTLV-II) was successfully amplified by the polymerase chain reaction...Full Text Available

1993-03-01

100

Evidence for increased in vitro recombination with insertion of human hepatitis B virus DNA.

AIM: To identify a method for efficient large-scale purification of functional hepatitis B virus polymerase (HBV-Pol) without addition of cellular factors.METHODS: Full-length HBV-Pol (843 amino...Full Text Available

2010-12-07

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

101

Effects of bovine leukemia virus infection on production and reproduction in dairy cattle.

Chromosomal translocation, deletion, and inversion/duplication directly linked to hepatitis B virus (HBV) DNA integration occur frequently in host DNA of human hepatocellular carcinomas. To test the...Full Text Available

1991-10-15

102

Detection of hepatitis B virus DNA among accepted blood donors in Nanjing, China

The purpose of this study was to determine the effects of bovine leukemia virus (BLV) infection on production, reproduction and longevity in dairy cattle. The study population was a commercial Holstein...Full Text Available

1992-10-01

103

Detection of Ockelbo virus RNA in skin biopsies by polymerase chain reaction.

BackgroundPosttransfusion hepatitis B virus (HBV) infection still occurs although its incidence has been substantially reduced since the introduction of screening of hepatitis B...Full Text Available

104

Dengue virus-specific murine T-lymphocyte proliferation: serotype specificity and response to recombinant viral proteins.

A sensitive assay based on the polymerase chain reaction for the detection of Ockelbo virus RNA was developed. Two primer pairs from the gene coding for the E2 glycoprotein were chosen. By use of a...Full Text Available

1993-08-01

105

Defective hepatitis B virus particles are generated by packaging and reverse transcription of spliced viral RNAs in vivo.

Definition of the T-lymphocyte responses to dengue viruses should aid in the development of safe and effective vaccines and help to explain the pathophysiology of dengue hemorrhagic fever and dengue...Full Text Available

1989-06-01

106

Correlates to Increased Lethality of Attenuated Venezuelan Encephalitis Virus Vaccine for Immunosuppressed Hamsters.

Generation of replicative defective viruses is frequently observed during viral infections. We now report that encapsidation and reverse transcription of spliced viral RNA is an additional mechanism...Full Text Available

1991-10-01

107

Cloning of the mouse hepatitis virus (MHV) receptor: expression in human and hamster cell lines confers susceptibility to MHV.

Splenectomy or pretreatment of adult hamsters with cyclophosphamide (Cytoxan) increased the lethality of the TC-83 vaccine strain of Venezuelan encephalitis virus (VEE), inoculated subcutaneously, from 12% for normal hamsters to 75 and 76% respectively. A...

1974-01-01

108

Chromosomal sites for hepatitis B virus integration in human hepatocellular carcinoma.

The cellular receptor for murine coronavirus mouse hepatitis virus (MHV)-A59 is a member of the carcinoembryonic antigen (CEA) family of glycoproteins in the immunoglobulin superfamily. We isolated...Full Text Available

1991-12-01

109

Characterization of the 5'-terminal cap structures of early simian virus 40 mRNA.

The discovery that hepatitis B virus (HBV) integrates into host chromosomes raises the question of whether such viral DNA integration correlates directly with the activation of specific oncogenes or...Full Text Available

1991-12-01

110

Bunyamwera virus can repair both insertions and deletions during RNA replication

Early simian virus 40-specific mRNA was isolated from lytically infected and stably transformed cells and analyzed with respect to the 5'-terminal cap content. An analogous diversity of capped structures...Full Text Available

1980-09-01

111

Biogeography of Tick-Borne Bhanja Virus (Bunyaviridae) in Europe

The genomic termini of RNA viruses contain essential cis-acting signals for such diverse functions as packaging, genome translation, mRNA transcription, and RNA replication, and thus...Full Text Available

2010-06-01

112

BK Virus and Human Cancer: Innocent until Proven Guilty

Bhanja virus (BHAV) is pathogenic for young domestic ruminants and also for humans, causing fever and affections of the central nervous system. This generally neglected arbovirus of the family Bunyaviridae...Full Text Available

2009-01-01

113

Avian Nephritis Virus (ANV) as a New Member of the Family Astroviridae and Construction of Infectious ANV cDNA

BK virus (BKV) is a polyomavirus that ubiquitously infects the human population. Following a typically subclinical primary infection, BKV establishes a lifelong persistent infection in the kidney...Full Text Available

2009-08-01

114

Antiviral Inhibition of Enveloped Virus Release by Tetherin/BST-2: Action and Counteraction

The complete RNA genome of the avian nephritis virus (ANV) associated with acute nephritis in chickens has been molecularly cloned and sequenced. Excluding the poly(A) tail, the genome comprises 6,927...Full Text Available

2000-09-01

115

A second origin of DNA plus-strand synthesis is required for optimal human immunodeficiency virus replication.

Tetherin (BST2/CD317) has been recently recognized as a potent interferon-induced antiviral molecule that inhibits the release of diverse mammalian enveloped virus particles from infected cells. By...Full Text Available

116

A nested case-control study of maternal-neonatal transmission of hepatitis B virus in a Chinese population

We recently reported that human immunodeficiency virus type 1 (HIV-1) unintegrated linear DNA displays a discontinuity in its plus strand, precisely defined by a second copy of the polypurine tract...Full Text Available

1992-05-01

117

A Rare Null Allele Potentially Encoding a Dominant-Negative TRIM5? Protein in Baka Pygmies

AIM: To examine the determinants of maternal-neonatal transmission of hepatitis B virus (HBV).METHODS: A nested case-control study was conducted in Changsha, Hunan, People’s Republic...Full Text Available

2011-08-21

118

Letter to the Editor: 1H, 13C and 15N chemical shift assignments of the capsid protein from Rous sarcoma virus

The global acquired immunodeficiency syndrome (AIDS) pandemic is thought to have arisen by the transmission of human immunodeficiency virus (HIV-1)-like viruses from chimpanzees in southeastern...Full Text Available

2009-08-15

119

Interaction Of Human La Protein With The Internal Ribosome Entry Site Of Hepatitis C Virus : Functional Role In Mediating Internal Initiation Of Translation

No abstract prepared.

1999-11-15

120

Indian Institute of Science Theses & Dissertations (India)

Full Text Available

2004-07-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

121

Fine mapping of a foot-and-mouth disease virus epitope recognized by serotype-independent monoclonal antibody 4B2.

The Acidic Domain of Hepatitis C Virus NS4A Contributes to RNA Replication and Virus Particle Assembly?

Full Text Available

2011-02-01

122

Sensitive enzyme immunoassay for detecting immunoglobulin M antibodies to Sindbis virus and further evidence that Pogosta disease is caused by a western equine encephalitis complex virus.

Hepatitis C virus NS3-4A is a membrane-bound enzyme complex that exhibits serine protease, RNA helicase, and RNA-stimulated ATPase activities. This enzyme complex is essential for viral genome replication...Full Text Available

2011-02-01

123

HTLV-3/STLV-3 and HTLV-4 Viruses: Discovery, Epidemiology, Serology and Molecular Aspects

An antibody capture enzyme immunoassay (EIA) was adapted for the detection of immunoglobulin M (IgM) antibody to Sindbis (SIN) virus. Sera from humans with a febrile illness characterized by rash and...Full Text Available

1985-10-01

124

Equine infectious anemia virus and human immunodeficiency virus DNA synthesis in vitro: characterization of the endogenous reverse transcriptase reaction.

Human T cell leukemia/lymphoma virus Type 1 and 2 (HTLV-1 and HTLV-2), together with their simian counterparts (STLV-1, STLV-2), belong to the Primate T lymphotropic viruses group (PTLV). The high percentage...Full Text Available

125

Elimination of L-A double-stranded RNA virus of Saccharomyces cerevisiae by expression of gag and gag-pol from an L-A cDNA clone.

The endogenous reverse transcriptase reaction of equine infectious anemia virus (EIAV) has been studied, and conditions allowing synthesis of full-length minus-strand DNA have been determined. In contrast...Full Text Available

1991-04-01

126

Ebola Virus VP40-Induced Particle Formation and Association with the Lipid Bilayer

We report that expression of a nearly full-length cDNA clone of the L-A double-stranded RNA virus causes virus loss in a wild-type strain of Saccharomyces cerevisiae. We show that in this system exclusion...Full Text Available

1993-05-01

127

Chemical and Immunological characterization of the major structural protein (p28) of MMC-1, a rhesus monkey endogenous type C virus: homology with the major structural protein of avian reticuloendotheliosis virus.

Viral protein 40 (VP40) of Ebola virus appears equivalent to matrix proteins of other viruses, yet little is known about its role in the viral life cycle. To elucidate the functions of VP40, we investigated...Full Text Available

2001-06-01

128

Characterization and engineering of sequences controlling in vivo synthesis of brome mosaic virus subgenomic RNA.

The major core protein (p28) of MMC-1, an endogenous type C virus of the rhesus monkey (Macaca mulatta), was purified and subjected to structural and immunological analyses. The NH2-terminal amino acid...Full Text Available

1981-09-01

129

CD5 Is Dissociated from the B-Cell Receptor in B Cells from Bovine Leukemia Virus-Infected, Persistently Lymphocytotic Cattle: Consequences to B-Cell Receptor-Mediated Apoptosis

Expression of brome mosaic virus (BMV) coat protein and internal genes of many other positive-strand RNA viruses requires initiation of subgenomic mRNA synthesis from specific internal sites on minus-strand...Full Text Available

1988-07-01

130

Towards a Systematic Construction of Realistic D-brane Models on a del Pezzo Singularity

Bovine leukemia virus (BLV), a retrovirus related to human T-cell leukemia virus types 1 and 2, can induce persistent nonneoplastic expansion of the CD5⁺ B-cell population, termed...Full Text Available

2001-02-01

131

Statistical Support Document for Proposed Effluent Limitations Guidelines and Standards for the Metal Products and Machinery Industry.

A systematic approach is followed in order to identify realistic D-brane models at toric del Pezzo singularities. Requiring quark and lepton spectrum and Yukawas from D3 branes and massless hypercharge, we are led to Pati-Salam extensions of the Standard Model. Hierarchies of masses, flavour mixings and control of couplings select higher order del Pezzo singularities, minimising the Higgs sector prefers toric del Pezzos with dP3 providing the most successful compromise. Then a supersymmetric local string model is presented with the following properties at low energies: (i) the MSSM spectrum plus a local B-L gauge field or additional Higgs fields depending on the breaking pattern, (ii) a realistic hierarchy of quark and lepton masses and (iii) realistic flavour mixing between quark and lepton families with computable CKM and PMNS matrices, and CP violation consistent with observations. In this construction, kinetic terms are ...

2011-01-01

132

Pyogenic abscess of the PSOAS - Description of cases registered in the university hospital of the valley

Table of Contents: Overview of Organization and Contents of Document; Analytical Data Collection Efforts and Definition of Options; Description of Data Conventions; Statistical Methodology: Modified Delta-Lognormal Model; Estimation Under the Modified Del...

2000-01-01

133

NASA - Informe De la Misi?n STS-126

In this chart review the authors describe 51 cases of PSOAS abscess treated at Hospital Universitario del Valle in Cali, Colombia in the last 10 years. It is not a rare condition and require high index of suspicion. epidemiology, bacteriology, diagnosis and treatment are discussed.

2006-06-01

134

NASA - COLABORACI?N INNOVATIVA REVOLUCIONAR? LA SUPERCOMPUTACION ...

17 Nov 2008 ... El Analizador de Carbono Org?nico Total ser? usado para probar muestras que vendr?n del nuevo sistema de Recobre de Agua. ...

135

Endometriosis: MedlinePlus tutorial interactivo del Instituto...

3 Ago 2004 ... Siguiendo las recomendaciones de la OSTP, una parte del nuevo sistema estar? disponible en la base general para asegurar que toda la ...

136

Method to generate the first design of the reload pattern to be used with the Presto-B code in the simulation of the CNLV U-1 reactor; Metodo para generar el primer diseno del patron de recarga a ser utilizado con el codigo Presto-B, en la simulacion del reactor de la CNLV U-1

Compartir Skip to Content Endometriosis Empezar Programa Interactivo de X-Plain Empezar Programa Interactivo de X-Plain Empezar el Programa de X-Plain Auto-operado Empezar el...

2011-04-27

137

Heparin binding sites on Ross River virus revealed by electron cryo-microscopy

This guide is applied for the reload pattern's formation for mirror symmetry of a core room and in accordance with the Control Cell core technique (of the english Control Cell Core - CCC) for the PRESTO-B code. (Author)

1992-08-15

138

Construction and Analytical Application of Internal Amplification Controls (IAC) for Detection of Food Supply Chain-Relevant Viruses by Real-Time PCR-Based Assays

Cell surface glycosaminoglycans play important roles in cell adhesion and viral entry. Laboratory strains of two alphaviruses, Sindbis and Semliki Forest virus, have been shown to utilize heparan sulfate as an attachment receptor, whereas Ross River virus (RRV) does not significantly interact with it. However, a single amino acid substitution at residue 218 in the RRV E2 glycoprotein adapts the virus to heparan sulfate binding and expands the host range of the virus into chicken embryo fibroblasts. Structures of the RRV mutant, E2 N218R, and its complex with heparin were determined through the use of electron cryo-microscopy and image reconstruction methods. Heparin was found to bind at the distal end of the RRV spikes, in a region of the E2 glycoprotein that has been previously implicated in cell-receptor recognition and antibody binding.

2005-02-20

139

British Library Electronic Table of Contents (United Kingdom)

Internal amplification controls (IACs) were constructed for incorporation into real-time nucleic acid amplification assays for bovine polyomavirus, hepatitis A virus, hepatitis E virus, human adenovirus, human norovirus genogroup I, human norovirus genogroup II, murine norovirus and porcine adenovirus. The addition of optimised amounts of IAC into the assays did not affect the limits of detection for each specific target virus. A poorly performed extraction of viral nucleic acids was simulated, and the effectiveness of IACs in identifying failed assays was demonstrated. The IACs constructed in this study can be reliably used in their specific assays to provide a robust control that can be routinely applied in the analysis of foods for viruses.

2011-01-01

140

The technical-economic potential of thermal energy saving in hospitals; El potencial tecnico-economico de ahorro de energia termica en hospitales

Lung studies with spiral CT. pitch 1 versus pitch 2; Confronto tra diversi parametri (pitch 1 e 2) nello studio del polmone con Tomografia Computerizzata spirale

The hospitals are important consumers of energy. At the General Hospital of Zone (HGZ) N of the IMSS in Aguscalientes (HGZ N . IMSS Ags.), the diesel oil is the main fuel that is used to satisfy the requirements of thermal energy of the hospital. According to the data collected by this author, this fuel represented in 2001, 75% of its total energy consumption and the 67.9% of its total costs in energy that ascended to $396.131 (December 2001 Dollars) Since this last amount represents an important percentage of the total expenses of the hospital (29.367 million dollars) it is important to determine the technical-economic possibilities of thermal energy saving of the hospital. The HGZ N 1 IMSS Ags. is located in a ampler conglomerate where the IMSS units are located, such as, the regional laundry, the sport unit, the center of social security, the familiar medicine unit N 1 and the hospital. Nevertheless departing from the installation of the electrical systems and the provision of steam ...

2001-07-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

141

Dentascan CT of mandibular incisive canal. Radiological anatomy and therapeutic implications; Tomografia computerizzata con Dentascan del canale incisivo mandibolare. Anatomia radiologica e implicazioni terapeutiche

In Spiral CT, the pitch is the ratio of the distance to tabletop travels per 360 degrees rotation to nominal slice width, expressed in mm. Performing Spiral CT examination with pitch 2 allows to reduce examination time, exposure and contrast dose, and X-ray tube overload. The authors investigated the yield of pitch 2 in lung parenchyma studies, particular relative to diagnostic image quality. [Italian] Nella Tomografia Computerizzata (TC) spirale il rapporto tra la distanza percorsa dal lettino portapaziente in una rotazione e lo spessore nominale dello strato, entrambi espressi in millimetri, si definische pitch. Se i due valori del rapporto coincidono si avra' pitch uguale a 1, mentre raddoppiando la distanza percorsa dal lettino portapaziente, per il medesimo spessore nominale, conseguentemente raddoppia il pitch (pitch 2). Eseguire gli esami TC spirale utilizzando pitch 2, consente di ridurre la durata dell'esame, la dose di esposizione, il ...

1999-03-01

142

Elimination of a new ampelovirus (GLRaV-Pr) and Grapevine rupestris stem pitting associated virus (GRSPaV) from two Vitis vinifera cultivars combining in vitro thermotherapy with shoot tip culture

The main purpose of this paper is to stress the importance of CT depiction of the mandibular incisive canal. This anatomical structure contains a major neurovascular bundle and is thus very important in the planning of mandibular implants in the canine-incisive area. The importance of careful assessment of the mandibular canal course before implantology is now widely recognized. The same holds true for the canine-incisive region in the jaw, to detect the incisive canal if present and prevent any complications from its accidental damage. CT with a dedicated software showed the incisive canal in a large number of patients, which calls for precise reporting of its presence, course, and relationships with teeth. [Italian] Scopo di questo lavoro e' sottolineare l'importanza di rilevare e segnalare la visibilita' con Tomografia Computerizzata del canale incisivo mandibolare, struttura anatomica al cui interno decorre un fascio vasculo nervoso e ...

1999-11-01

143

British Library Electronic Table of Contents (United Kingdom)

A new virus species designated as Grapevine leafroll associated virus-Pr (GLRaV-Pr), which is classified in a distinct phylogenetic group of the genus Ampelovirus (Closteroviridae), was recently characterized from Greek grapevine cultivars. Elimination studies of GLRaV-Pr were carried out in two grapevine cultivars, 'Mantilaria' and 'Prevezaniko', co-infected with Grapevine rupestris stem pitting associated virus (GRSPaV, Flexiviridae). Both viruses were detected by nested RT-PCR assays. Virus elimination was achieved by combining in vitro thermotherapy with meristem ( 0.2mm) or shoot tip culture ( 0.5cm). The survival and regeneration rate of meristems was very low. On the other hand, high survival rates were observed in the cultured shoot tips accompanied with high elimination rates for ...

2009-01-01

144

Absence of detectable xenotropic murine leukemia virus-related virus in plasma or peripheral blood mononuclear cells of human immunodeficiency virus Type 1-infected blood donors or individuals in Africa

British Library Electronic Table of Contents (United Kingdom)

BACKGROUND: Since the identification of xenotropic murine leukemia virus-related virus (XMRV) in prostate cancer patients in 2006 and in chronic fatigue syndrome patients in 2009, conflicting findings have been reported regarding its etiologic role in human diseases and prevalence in general populations. In this study, we screened both plasma and peripheral blood mononuclear cells (PBMNCs) collected in Africa from blood donors and human immunodeficiency virus Type 1 (HIV-1)-infected individuals to gain evidence of XMRV infection in this geographic region. STUDY DESIGN AND METHODS: A total of 199 plasma samples, 19 PBMNC samples, and 50 culture supernatants from PBMNCs of blood donors from Cameroon found to be infected with HIV-1 and HIV-1 patients from Uganda were screened for XMRV infecti...

2011-01-01

145

Phase diagram and effective shape of semi-flexible colloidal rods and biopolymers

We study suspensions of semi-flexible colloidal rods and biopolymers using an Onsager-type second-virial functional for a segmented-chain model. For suspensions of thin and thick fd virus particles we calculate phase diagrams in quantitative agreement with experimental observations, and we find their effective state-point dependent shape to be much shorter and thicker than the actual shape. We also calculate the stretching of worm-like micelles in a host fd virus solution, again finding agreement with experiments. For both systems, our results show that the fd virus stiffness can play a key role in system behavior.

2011-01-01

146

The dengue viruses.

The Factors Determining the Level of Virus Production. Report ...

Dengue, a major public health problem throughout subtropical and tropical regions, is an acute infectious disease characterized by biphasic fever, headache, pain in various parts of the body, prostration,...Full Text Available

1990-10-01

147

Targeted integration of baboon endogenous virus in the BEVI locus on human chromosome 6.

... After formation of the monolayer (usually in 48 hours), the growth medium was poured off, and the cells washed twice with a Henkes solution and ...

1971-12-02

148

Series of surveys for enteric viruses and indicator organisms in Tokyo Bay after an event of combined sewer overflow.

The infection of cultured human cells with baboon endogenous virus (BEV) frequently leads to an association of viral DNA with a specific genetic locus (termed BEVI, for baboon endogenous virus infection) on chromosome 6. Restriction endonuclease digestion of DNA from BEV-infected human cells and their derived somatic cell clones frequently revealed a common cellular DNA sequence in the proximity of one of the junctions between cellular DNA and the integrated virus. We propose that a short cellular DNA sequence, repeated on chromosome 6 and separated by unique DNA sequences, presents a high-affinity target for the integration of BEV in human cells. PMID:6401843

1983-01-13

149

Novel Cytotoxic Vectors Based on Adeno-Associated Virus

Combined sewer overflows (CSOs) have been recognised as one of the serious sources of pollution to the water environment during rain events, although field surveys to investigate the effect of their magnitude and duration on receiving waters have been very limited. The fates of enteric viruses (norovirus G1, G2, enteroviruses) and coliforms were determined in the wastewater treatment plant on a fine day and on a rainy day. Not all microorganisms were reduced in the primary treatment, but were reduced in the secondary treatment. Occurrences of enteric viruses and levels of coliforms were surveyed in the receiving coastal area after a CSO event, with the profiles of the enteric viruses in the coastal seawater being almost at the same positive ratio for 4 d after the CSO event. PMID:15318519

2004-01-01

150

Wastenet

positive primary PymT breast cancer cells in primary co-cultured tumor tissue, suggesting target specificity of

151

Nosocomial Vaccinia Infection

Human viral gastroenteritis.

Although hospital-associated spread of vaccinia has been reported in the past, there have been no recent reports. This paper describes hospital-associated spread of vaccinia virus infection, supplies...Full Text Available

1976-10-01

152

Human endogenous retroviruses: transposable elements with potential ?

During the last 15 years, several different groups of fastidious viruses that are responsible for a large proportion of acute viral gastroenteritis cases have been discovered by the electron microscopic...Full Text Available

1989-01-01

153

Effect of Diaporthe RNA virus 1 (DRV1) on growth and pathogenicity of different Diaporthe species

Human endogenous retroviruses (HERVs) are a significant component of a wider family of retroelements that constitute part of the human genome. These viruses, perhaps representative of previous exogenous...Full Text Available

2004-10-01

154

British Library Electronic Table of Contents (United Kingdom)

A 4.1?kbp positive-strand RNA virus known as Diaporthe RNA virus 1 (DRV1) occurs in hypovirulent, non-sporulating isolates of the fungal pathogen Diaporthe perjuncta. A full-length cDNA clone of DRV1 was developed and RNA transcribed from the cDNA clone used to transfect different Diaporthe spp. The transfected species included three D. ambigua isolates and an unidentified Phomopsis asexual state of a Diaporthe sp. Successful transfections were confirmed using RT-PCR. Although the in vitro-transcribed positive sense single-stranded RNA used for transfection included vector sequences at both ends, the genomes of progeny virus from DRV1-transfected isolates were free of the vector sequences. Transfection resulted in morphological changes in these fungal pathogens. However, the presence of DR...

2011-01-01

155

Defense.gov News Article: DARPA Effort Speeds Biothreat ...

Cuba's response to the HIV epidemic.

... H1N1 a pandemic virus in June 2009, the Defense Advanced Research Projects Agency had a plan to address the crisis. The DARPA effort, called ...

156

Clinical, virological and immunological responses in Danish HIV patients receiving raltegravir as part of a salvage regimen

BACKGROUND: Cuba's response to the human immunodeficiency virus (HIV) epidemic has been to conduct mass testing of the population to ascertain seroprevalence, to enforce mandatory relative quarantine...Full Text Available

1991-05-01

157

Analysis of codon usage and nucleotide composition bias in polioviruses

Raltegravir is the first integrase inhibitor approved for treatment of HIV-infected patients harboring multiresistant viruses.

2010-01-01

158

Analysis of cerebrospinal fluid from chronic fatigue syndrome patients for multiple human ubiquitous viruses and xenotropic murine leukemia-related virus

BackgroundPoliovirus, the causative agent of poliomyelitis, is a human enterovirus and a member of the family of Picornaviridae and among the most rapidly evolving viruses known....Full Text Available

159

British Library Electronic Table of Contents (United Kingdom)

Abstract Recent reports showed many patients with chronic fatigue syndrome (CFS) harbor a retrovirus, xenotropic murine leukemia-related virus (XMRV), in blood; other studies could not replicate this finding. A useful next step would be to examine cerebrospinal fluid, because in some patients CFS is thought to be a brain disorder. Finding a microbe in the central nervous system would have greater significance than in blood because of the integrity of the blood-brain barrier. We examined cerebrospinal fluid from 43 CFS patients using polymerase chain reaction techniques, but did not find XMRV or multiple other common viruses, suggesting that exploration of other causes or pathogenetic mechanisms is warranted. Ann Neurol 2011;

2011-01-01

160

A Transposon and Transposase System for Human Application

Diagnostic imaging and interventional radiology of the amoebic liver abscess. Personal experience; Diagnostica per immagini e radiologia interventistica degli ascessi amebici del fegato: esperienza personale

The stable introduction of therapeutic transgenes into human cells can be accomplished using viral and nonviral approaches. Transduction with clinical-grade recombinant viruses offers the potential...Full Text Available

2010-04-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

161

Comparative study of the electrochemical behaviour of iron in alkaline media containing red mud and grey slurries. the effect of Al{sup 3}+; Estudio comparativo del comportamiento electroquimico del hierro en medio alcalino en presencia de lodos rojos y lodos grises. Efecto del Al{sup 3}+

The diagnostic imaging findings in hepatic amoebiasis and the capabilities of percutaneous drainage have already been described but some debate is open on both diagnosis and treatment. It is reported the experience with the ultrasound (US) and Computed Tomography (CT) studies of the hepatic amoebic abscess and its management. [Italian] I reperti con diagnostica per immagini nell'amebiasi epatica e le possibilita' del drenaggio percutaneo sono stati gia' illustrati; esistono tuttavia controversie sul piano sia diagnostico che terapeutico. Obiettivo di questo lavoro e' quello di riportare l'esperienza personale nella diagnostica con ecografia e tomografia computerizzata (TC) e nel trattamento degli ascessi amebici del fegato.

1999-10-01

162

Validation of a new software version for monitoring of the core of the Unit 2 of the Laguna Verde power plant with ARTS; Validacion de una nueva version del software para monitoreo del nucleo de la Unidad 2 de la Central Laguna Verde con ARTS

Red mud (Rm) is the main by-product of the Bayer process for alumina extraction from bauxite, and grey slurries (GS) are generated during the granite rocks transformation processes. the huge volume of those wastes generated every year suppose a serious environmental problem. Recent works by our group demonstrate the efficiency of both types of muds as corrosion inhibitors for iron and steel in alkaline media containing chlorides. The elemental analysis of these slurried shows that Al{sup 3}+ is the main common element. Therefore, the aim of this paper is to study the effect of the soluble Al{sup 3}+ in the electrochemical behaviour of iron in alkaline media, in presence and absence of chlorides. The electrochemical study using Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) together with surface analysis techniques allowed to conclude that Al{sup 3}+ blocks the surface of steel in alkaline media chloride increasing the steel resistance to chlorides attack. ...

2009-07-01

163

Scientific information and public opinion. Daily press analysis about ozone hole in the years 1996-1997; Informazione scientifica e opinione pubblica. Analisi della stampa quotidiana italiana negli anni 1996-1997 sul tema del buco dell'ozono

In this work it is intended a methodology to validate a new version of the software used for monitoring the reactor core, which requires of the evaluation of the thermal limits settled down in the Operation Technical Specifications, for the Unit 2 of Laguna Verde with ARTS (improvements to the APRMs, Rod Block Monitor and Technical specifications). According to the proposed methodology, those are shown differences found in the thermal limits determined with the new versions and previous of the core monitoring software. Author)

2005-07-01

164

CT colonoscopy with volume rendering. Results and comparison with endoscopy and surgery; Cononscopia con tomografia computerizzata con ricostruzioni di volume: risultati e confronto con endoscopia

One of ENEA's many activities consists in dealing with the analysis of information strategies. In this case the analysis concerns an environmental issue, the ozone hole which, in spite of the consideration given by the media, is not sufficiently highlighted, as well as in the case of climate changes issue. A survey of the coverage of four daily newspapers was realized; the time period covered by the analysis spans form January 1996 to December 1997, 77 articles in total. The newspapers involved are: La Repubblica, Il Corriere della Sera, L'Unita' on a national scale, and Il Resto del Carlino on a regional scale. The number of articles analyzed is divided as follows: 26 for the Repubblica, 24 for the Corriere della Sera, 21 for the Unita', 6 for the Resto del Carlino. The purpose of the work was to detect quality and quantity indicators of the Italian press about the ozone hole issue and possible environmental ...

1999-07-01

165

A case of subfascial lipoma in the lateral space of neck: US and CT findings

The paper reports the results of a preliminary experience with volume rendering technique of virtual computerized tomography colonoscopy and compares it with endoscopy and surgery showing its capability in detecting colon and rectal cancers. [Italian] Scopo del lavoro e' riportare l'esperienza personale preliminare nell'uso della colonscopia TC virtuale confrontando i risultati con quelli dell'endoscopia e dei riscontri operatori, dimostrandone l'efficacia nella diagnosi delle malattie tumorali del colon-retto.

1999-09-01

166

British Library Electronic Table of Contents (United Kingdom)

Sommario I lipomi del collo e della testa in genere si verificano nel tessuto sottocutaneo superficiale. Quelli sottofasciali intramuscolari sono molto rari. Riportiamo di seguito il caso di una donna di 53 anni con un lipoma sottofasciale localizzato nello spazio antero-laterale del collo. La diagnosi della lesione e stata ottenuta con esame clinico e confermata con Ecografia (US) e Tomografia Computerizzata (TC). La lesione, caratterizzata da capsula e aspetto fibrolipomatoso, e stata escissa con intervento chirurgico.

2009-01-01

167

CT assessment of thoracic gunshot wounds. Our personal experience; Ruolo della Tomografia Computerizzata nelle lesioni da arma da fuoco del torace. Esperienza personale

Wild-type p53 is not a negative regulator of simian virus 40 DNA replication in infected monkey cells.

CT is a valuable tool in assessing thoracic gunshot wounds. CT is also the method of choice in emergency, because it permits rapid depiction of bullet damage to the chest and to other body districts. This in turn permits correct assessment of the main thoracic injuries, plus adequate and prompt planning of surgical treatment or support intensive care. It is reported on the role of CT in diagnosing the complex pleuropulmonary, cardiovascular and thoracic wall injuries caused by gunshot wounds, with their specific and acute signs which differ greatly fro those of other types of chest trauma. In the last 4 years, it was observed an amount of 76 cases of gunshot injury, twenty-six of them involved the chest. The patients, 25 men and 1 woman (mean age: 32 years, range: 17-48), were all submitted to emergency CT with iv contrast agent injection and the CT-angiography technique. The reanimator was always presented to monitor the patients' vital functions and shock state. CT of the ...

1999-11-01

168

Viral suppression of multiple escape mutants by de novo CD8⁺T cell responses in a human immunodeficiency virus-1 Infected elite suppressor

To analyze the proposed growth-inhibitory function of wild-type p53, we compared simian virus 40 (SV40) DNA replication in primary rhesus monkey kidney (PRK) cells, which express wild-type p53, and...Full Text Available

1993-02-01

169

Unusual resistance to ionizing radiation of the viruses of kuru, Creutzfeldt-Jakob disease, and scrapie.

Elite suppressors or controllers (ES) are HIV-1 infected patients who maintain undetectable viral loads without treatment. While HLA-B*57-positive ES are usually infected with virus that is unmutated...Full Text Available

170

Tissue tropism and neuroinvasion of West Nile virus do not differ for two mouse strains with different survival rates

The titers of several preparations of kuru. Creutzfeldt-Jacob disease, and scrapie viruses were reduced by only 1/10th or less by high doses of gamma radiation of 50 kGy and by only 1/10th-1/1000th...Full Text Available

1978-12-01

171

Spatial Risk Assessments Based on Vector-Borne Disease Epidemiologic Data: Importance of Scale for West Nile Virus Disease in Colorado

West Nile virus (WNV) is a mosquito-borne flavivirus that infects the central nervous system of humans and other animals. In this study, we found that C3H/HeN (C3H) mice exhibited a higher morbidity...Full Text Available

2007-11-25

172

Safety and immunogenicity of ERA strain of rabies virus propagated in a BHK-21 cell line.

We used epidemiologic data for human West Nile virus (WNV) disease in Colorado from 2003 and 2007 to determine 1) the degree to which estimates of vector-borne disease occurrence is influenced by spatial...Full Text Available

2010-05-01

173

Reversible conformational change in herpes simplex virus glycoprotein B with fusion-from-without activity is triggered by mildly acidic pH

The ERA strain of rabies virus was propagated in a baby hamster kidney cell line (BHK-21/C13). The viral titer was 10(1.8) tissue culture infective doses (TCID) higher than that of commercial ERA vaccine....Full Text Available

1989-10-01

174

Radial distribution of dose and cross-sections for the inactivation of dry enzymes and viruses

BackgroundThe pre-fusion form of the herpes simplex virus (HSV) fusion protein gB undergoes pH-triggered conformational change in vitro and during viral entry (Dollery...Full Text Available

175

Quantitation of transcribing native simian virus 40 minichromosomes extracted from CV1 cells late in infection.

A new semi-empirical algorithm for the radial distribution of dose is compared with available data. The algorithm is used to calculate the inactivation cross section for dry enzymes and viruses using an extended target model of a 1-hit detector. Agreement with data is at about the 15% level, approximating the precision of the data itself. (author).

1985-05-01

176

Presence of antibodies to a putatively immunosuppressive part of human immunodeficiency virus (HIV) envelope glycoprotein gp41 is strongly associated with health among HIV-positive subjects.

Simian virus 40 transcriptional complexes could be extracted from CV1 cells late in infection and separated from the bulk of inactive viral chromatin. Sucrose gradient sedimentation, cesium sulfate...Full Text Available

1981-04-01

177

Prediction of conformational changes by single mutation in the hepatitis B virus surface antigen (HBsAg) identified in HBsAg-negative blood donors

The IgG response to gp41 (envelope glycoprotein of Mr 41,000) of the human immunodeficiency virus (HIV) was studied with eight synthetic peptides derived from three different regions of the protein....Full Text Available

1988-07-01

178

Overview of the activity of a Brucella abortus preparation, Bru-Pel.

BackgroundSelection of hepatitis B virus (HBV) by host immunity has been suggested to give rise to variants with amino acid substitutions at or around the 'a' determinant...Full Text Available

179

Morphological, Histochemical, Immunohistochemical, and Ultrastructural Characterization of Tumors and Dysplastic and Non-Neoplastic Lesions Arising in BK Virus/tat Transgenic Mice

The properties of a nonviable, aqueous ether-extracted Brucela abortus preparation, Bru-Pel, are described. In addition to inducing a "virus-type" interferon response and protecting mice against challenge with otherwise lethal doses of Semliki Forest virus, Bru-Pel is demonstrated to have potent antitumor properties in mice. These antitumor effects appear to be mediated by an increase in nonspecific resistance similar to that seen with other experimental antitumor agents. PMID:728911

1978-11-01

180

Long-Term Specific Immune Responses Induced in Humans by a Human Immunodeficiency Virus Type 1 Lipopeptide Vaccine: Characterization of CD8⁺-T-Cell Epitopes Recognized

To study the role in AIDS pathogenesis of the human immunodeficiency virus type 1 (HIV-1) Tat protein, a transactivator of viral and cellular genes, we generated transgenic mice with a recombinant DNA...Full Text Available

1999-04-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

181

Local and systemic antibody response to bovine respiratory syncytial virus infection and reinfection in calves with and without maternal antibodies.

We studied the effect of booster injections and the long-term immune response after injections of an anti-human immunodeficiency virus type 1 (HIV-1) lipopeptide vaccine. This vaccine was injected alone...Full Text Available

2003-10-01

182

Interferon-? expressed by a rabies virus-based HIV-1 vaccine vector serves as a molecular adjuvant and decreases pathogenicity

Enzyme-linked immunosorbent assays for the detection of immunoglobulin M (IgM), IgA, IgG1, and IgG2 antibodies against bovine respiratory syncytial virus (BRSV) were used to measure antibody responses...Full Text Available

1987-06-01

183

Induction of Antibody Responses to African Horse Sickness Virus (AHSV) in Ponies after Vaccination with Recombinant Modified Vaccinia Ankara (MVA)

Type I interferon is important in anti-viral responses and in coordinating the innate immune response. Here we explore the use of interferon-β to adjuvant the response to a rabies virus...Full Text Available

2008-12-20

184

Hepatitis C Virus RNA Replication Requires a Conserved Structural Motif within the Transmembrane Domain of the NS5B RNA-Dependent RNA Polymerase ?

BackgroundAfrican horse sickness virus (AHSV) causes a non-contagious, infectious disease in equids, with mortality rates that can exceed 90% in susceptible horse populations....Full Text Available

185

Features of two hepatitis B virus (HBV) DNA integrations suggest mechanisms of HBV integration.

Hepatitis C virus (HCV) nonstructural protein 5B (NS5B), the viral RNA-dependent RNA polymerase (RdRp), is a tail-anchored protein with a highly conserved C-terminal transmembrane domain (TMD) that...Full Text Available

2010-11-01

186

Expression of alternatively spliced human T-lymphotropic virus type I pX mRNA in infected cell lines and in primary uncultured cells from patients with adult T-cell leukemia/lymphoma and healthy carriers.

Two integrated hepatitis B virus (HBV) DNA molecules were cloned from two primary hepatocellular carcinomas each containing only a single integration. One integration (C3) contained a single linear...Full Text Available

1989-06-01

187

Establishment of stable Huh-7 cell lines expressing various hepatitis C virus genotype 3a protein: an in-vitro testing system for novel anti-HCV drugs

Although human T-cell lymphotropic virus type I (HTLV-I) is the etiologic agent of adult T-cell leukemia/lymphoma (ATL), the role of viral gene expression in the progression to and maintenance of the...Full Text Available

1992-04-01

188

Duck Egg-Drop Syndrome Caused by BYD Virus, a New Tembusu-Related Flavivirus

BackgroundHepatitis C virus (HCV) infection is the leading cause of chronic hepatitis which progresses to hepatocellular carcinoma (HCC) afflicting > 170 million people worldwide....Full Text Available

189

Detecting Sugarcane yellow leaf virus infection in asymptomatic leaves with hyperspectral remote sensing and associated leaf pigment analysis

Since April 2010, a severe outbreak of duck viral infection, with egg drop, feed uptake decline and ovary-oviduct disease, has spread around the major duck-producing regions in China. A new virus, named...Full Text Available

190

Dengue Plaque Reduction Neutralization Test (PRNT) in Primary and Secondary Dengue Virus Infections: How Alterations in Assay Conditions Impact Performance

Sugarcane yellow leaf caused by Sugarcane yellow leaf virus (ScYLV) does not produce visual symptoms in most susceptible plants until late in the growing season. An experiment was conducted to determine if leaf reflectance and pigment analysis could be used to determine ScYLV infection prior to sym...

191

Comparative Study of ¹²⁵I- and [³H]Acetate-Labeled Antibodies in Detecting Iridescent Viruses

Dengue virus (DENV) infection is a worsening global health problem. The plaque reduction neutralization test (PRNT) is currently considered to be the “gold standard” to characterize...Full Text Available

2009-11-01

192

Chimeric Matrix Proteins Encoded by Defective Proviruses with Large Internal Deletions in Human T-Cell Leukemia Virus Type 1-Infected Humans

Radioimmunoassays for detecting cell-associated or released virus are described using either ¹²⁵I- or [³H]acetate-labeled antibodies. In the first assay system, antigen-antibody...Full Text Available

1974-07-01

193

Antigenic drift in influenza virus H3 hemagglutinin from 1968 to 1980: multiple evolutionary pathways and sequential amino acid changes at key antigenic sites.

Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent of adult T-cell leukemia/lymphoma (ATLL), HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), and other diseases....Full Text Available

2000-05-01

194

Analysis of the transcript encoding the latent Epstein-Barr virus nuclear antigen I: a potentially polycistronic message generated by long-range splicing of several exons.

Surveys of the antigenic properties of a wide range of variants of the H3N2 (Hong Kong) influenza virus subtype have revealed complex patterns of variants cocirculating during each of the main epidemic...Full Text Available

1983-10-01

195

A single-stranded gap in human immunodeficiency virus unintegrated linear DNA defined by a central copy of the polypurine tract.

The Epstein-Barr virus nuclear antigen (EBNA I) present in latently infected cells is encoded in a 2-kilobase exon contained in the BamHI K viral genomic fragment. This exon is, however, found within...Full Text Available

1985-12-01

196

??⁺ T-Lymphocyte Cytotoxicity against Envelope-Expressing Target Cells Is Unique to the Alymphocytic State of Bovine Leukemia Virus Infection in the Natural Host

The structure of unintegrated human immunodeficiency virus type 1 (HIV-1) DNA from acutely infected human lymphoid cells was analyzed by nuclease S1 cleavage. We observed a unique, discrete single-stranded...Full Text Available

1991-05-01

197

Investigation of xenotropic murine leukemia virus-related virus (XMRV) in human and other cell lines.

Bovine leukemia virus (BLV) is a complex B-lymphotrophic retrovirus of cattle and the causative agent of enzootic bovine leukosis. Serum antibody in infected animals does not correlate with protection...Full Text Available

2000-09-01

198

First report of the complete sequence of Sida golden yellow vein virus from Jamaica.

Xenotropic murine leukemia virus-related virus (XMRV) was discovered in human prostate tumors and later in some chronic fatigue syndrome (CFS) patients. However, subsequent studies have identified various sources of potential contamination with XMRV and other murine leukemia virus (MLV)-related sequences in test samples. Biological and nucleotide sequence analysis indicates that XMRV is distinct from known xenotropic MLVs and has a broad host range and cell tropism including human cells. Therefore, it is prudent to minimize the risk of human exposure to infection by evaluating XMRV contamination in cell lines handled in laboratory research and particularly those used in the manufacture of biological products. Nested DNA PCR assays were optimized for investigating XMRV gag and env sequences in various cell lines, which included MRC-5, Vero, HEK-293, MDCK, HeLa, and A549, that may be used in the development of some vaccines ...

2011-10-11

199

Evolutionary dynamics of Newcastle disease virus

Begomoviruses are phytopathogens that threaten food security [18]. Sida spp. are ubiquitous weed species found in Jamaica. Sida samples were collected island-wide, DNA was extracted via a modified Dellaporta method, and the viral genome was amplified using degenerate and sequence-specific primers [2, 11]. The amplicons were cloned and sequenced. Sequence analysis revealed that a DNA-A molecule isolated from a plant in Liguanea, St. Andrew, was 90.9% similar to Sida golden yellow vein virus-[United States of America:Homestead:A11], making it a strain of SiGYVV. It was named Sida golden yellow vein virus-[Jamaica:Liguanea 2:2008] (SiGYVV-[JM:Lig2:08]). The cognate DNA-B, previously unreported, was successfully cloned and was most similar to that of Malvastrum yellow mosaic Jamaica virus (MaYMJV). Phylogenetic analysis suggested that this virus was most closely related to begomoviruses that infect ...

2011-05-29

200

The use of molecular biology techniques for the diagnosis and epidemiological study of foot-and-mouth disease virus in Thailand

A comprehensive dataset of NDV genome sequences was evaluated using bioinformatics to characterize the evolutionary forces affecting NDV genomes. Despite evidence of recombination in most genes, only one event in the fusion gene of genotype V viruses produced evolutionarily viable progenies. The codon-associated rate of change for the six NDV proteins revealed that the highest rate of change occurred at the fusion protein. All proteins were under strong purifying (negative) selection; the fusion protein displayed the highest number of amino acids under positive selection. Regardless of the phylogenetic grouping or the level of virulence, the cleavage site motif was highly conserved implying that mutations at this site that result in changes of virulence may not be favored. The coding sequence of the fusion gene and the genomes of viruses from wild birds displayed higher yearly rates of change in virulent viruses than in ...

2009-08-15

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

201

The detection of foot-and-mouth disease (FMD) virus from various kinds of field samples (tissue extract and cell culture isolate) was studied using the polymerase chain reaction (PCR) technique. The gene selected for diagnosis was the polymerase gene and an amplification target product of 454 bp in length was produced using AP5/AP6 primer sets. The PCR product was further examined by NcoI endonuclease digestion. The presence of the internal restriction site was confirmed by demonstration of two small fragments of 330 bp and 124 bp in length. Forty-nine samples that gave positive and negative results by ELISA typing and were positive by the PCR test were tested by NcoI digestion to confirm the results. About 10% of PCR products could not be confirmed by the method. Furthermore the FMD RNA polymerase gene could be detected by the PCR method in samples negative in both ELISA typing and the virus isolation test. A total of 23 samples were examined ...

2000-05-01

202

lla4892q.305

Structure of Mesita del Buey at TA-54, Los Alamos National Laboratory, New Mexico

PeDEL GRcXPmDtlSEZ`H==>T32\\ZtrPOP6tM UKwNEH5|J?:O TONRHwIN O1?)J`MiO\\ ?CQBYIL robb_M RSM7A8: qL6;90f44:7\\I07QO AE/U U_PbqIBYc6mTL?:2NDerX=Z =P<;K aRITGUN ...

203

III International Symposium on analytical methodology in the Environment. III Symposium Internacional de Metodologia Analitica en el campo del medio ambiente

The geological structure of Mesita del Buey at Technical Area 54 (TA-54) was examined using precise surveying of the contact between units 1v and 2 of the Tshirege Member of the Bandelier Tuff at 3.5 km along the north wall of Pajarito Canyon and 0.6 km along the north wall of a tributary to Canada del Buey. Estimated structure contours on this contact indicate typical strikes of N40E to N70E along this part of Mesita del Buey, although the apparent strike of the tuff is E-W at the western part of the survey. Typical dips are 1.0{degree} to 2.0{degree} to the east or southeast, with an estimated maximum dip of 3.2{degree} near the west end of Material Disposal Area G. Thirty seven faults with vertical displacements of 5 to 65 cm were observed in outcrop along the Pajarito Canyon traverse, and, due to the incomplete exposure of the unit 1v-unit 2 contact, many more faults of this magnitude undoubtedly exist. The faults have ...

1998-04-23

204

Contrast-enhanced helical CT in sigmoid diverticulitis. Findings and impact on diagnosis and treatment; Ruolo della tomografia computerizzata spirale nella diverticolite del sigma e implicazioni diagnostico-terapeutiche

The symposium had four areas in analytical methodology to study global environmental change: air; water, wastes and speciation, analytical methodology and environmental management. The symposium had 24 conferences and papers.

1994-01-01

205

Proposal to neutralize acid fluids from wells in the Los Humeros, Pue., geothermal field; Propuesta para la neutralizacion de fluidos acidos provenientes de pozos del campo geotermico de Los Humeros, Pue.

Aim of this retrospective study was to assess the capability of contrast-enhanced helical computerised tomography (CT) in sigmoid diverticulitis, especially relative to selection of the appropriate treatment. The findings of 41 patients were reviewed from 1998 to 1999 June. Contrast-enhanced helical CT is the imaging modality of choice in assessing mild or severe acute diverticulitis because it provides useful information for appropriate treatment planning in the emergency setting. This technique is most useful in questionable cases and in patients with suspected severe diverticulitis where a choice must be made between different treatment options. [Italian] Scopo di questo studio retrospettivo e' illustrare i molteplici aspetti radiologici della flogosi diverticolare sigmoidea con tomografia computerizzata (TC) spirale, con specifico riguardo alle implicazioni terapeutiche che scaturiscono dall'analisi e dal confronto dei reperti TC con i riscontri clinici e ...

2000-03-01

206

Ionospheric response to the phenomena occurring below and above it: a summary

Neutralizing an acidic fluid consists of adding a sodium hydroxide solution to neutralize the H group of acids, therefore increasing the pH. The injection of sodium hydroxide has to be continuous and at a proper depth inside the well to protect against the corrosion of casing and surface equipment. Neutralization is a common practice used in geothermal fields, such as at The Geysers in the US and Miravalles in Costa Rica-places where aggressive fluids cause problems for extracting and using geothermal fluids commercially. A zone surrounding wells H-4, H-16 and H-29 in the northern section of the Los Humeros, Pue., geothermal field, known as the Colapso Central, has shown evidence of aggressively acidic fluids. Several wells drilled in the area had to be repaired, thus plugging and isolating the deepest production zones. Well H-43 was drilled two years ago in the northern zone of the field, and even though it is not located in the aggressive-fluid zone, the well presents mineralogical ...

2009-07-15

207

Phylogeography of rabies virus isolated from dogs in Brazil between 1985 and 2006

Some of well known F{sub 2} layer anomalies might have their origin in lower atmosphere and some have extra terrestrial sources. It has been suggested that the phenomena occurring below the ionosphere such as thunderstorms, lightning/sprites, volcanic eruptions and earthquakes and above the phenomena like corona mass ejection, solar flares and extra terrestrial events may produce F{sub 2} layer signatures. In the present paper we have tried to summarize the effect of some phenomena occurring below the ionosphere like thunderstorms, lightning/sprites and seismic activity and the phenomena such as solar flares which occur above the ionosphere, on the ionospheric electron and ion temperatures. The paper also discusses possibilities. The ionospheric electron and ion temperatures were measured by the RrPAa payload aboard the Iindian SRrOSS-C2 satellite. The normal day's ion and electron temperatures have been compared to the temperatures recorded during the disturbed day. The ...

2009-01-15

208

British Library Electronic Table of Contents (United Kingdom)

To establish the phylogeographic relationships in rabies viruses in Brazil, we studied a dataset retrieved from GenBank consisting of 71 genetic sequences from the coding region of the N gene of rabies viruses isolated in dogs over a period of 22?years. The Bayesian Markov chain Monte Carlo method available in the BEAST package was used with the GTR+G+?4 evolutionary model in conjunction with the relaxed uncorrelated lognormal molecular clock model and an exponential growth tree prior. A discrete phylogeographic diffusion model was also analyzed using a standard continuous-time Markov chain viewed with Google Earth to provide a spatial projection of the diffusion of genetic lineages based on their phylogeographic relationships. The topology of the time and substitution phylogenetic trees a...

2011-01-01

209

Bioinformatic analysis of BBTV satellite DNA in Hainan

British Library Electronic Table of Contents (United Kingdom)

Banana bunchy top virus (BBTV), family Nanaviridae, genus Babuvirus, is a single stranded DNA virus (ssDNA) that causes banana bunchy top disease (BBTD) in banana plants. It is the most common and most destructive of all viruses in these plants and is widespread throughout the Asia-Pacific region. In this study we isolated, cloned and sequenced a BBTV sample from Hainan Island, China. The results from sequencing and bioinformatics analysis indicate this isolate represents a satellite DNA component with 12 DNA sequences motifs. We also predicted the physical and chemical properties, structure, signal peptide, phosphorylation, secondary structure, tertiary structure and functional domains of its encoding protein, and compare them with the corresponding quantities in the replication initiatio...

2011-01-01

210

A Hypothesis: Supplementation with Mushroom-Derived Active Compound Modulates Immunity and Increases Survival in Response to Influenza Virus (H1N1) Infection.

Non cardiopatic and cardiopatic beta thalassaemic patients: quantitative and qualitative cardiac iron deposition evaluation with MRI; Pazienti {beta} talassemici non cardiopatici e cardiopatici: valutazione quantitativa e qualitativa del deposito di ferro cardiaco con RM

We hypothesize that the mushroom-derived active compound may be a potential strategy for increasing survival in response to influenza virus (H1N1) infection through the stimulation of host innate immune response. The validity of the hypothesis can be tested by immune response to influenza infection as seen through survival percentage, virus clearance, weight loss, natural killer cell cytotoxicity, Tumor Necrosis Factor-? (TNF-?) and Interferon-gamma (IFN-?) levels, lytic efficiency in the spleens of mice and inducible nitric oxide synthase mRNA expressions in RAW 264.7 murine macrophage cells. The hypothesis may improve people's quality of life, reduce the medical cost of our healthcare system and eliminate people's fears of influenza outbreak. PMID:21660092

2011-03-20

211

When autophagy meets viruses: a double-edged sword with functions in defense and offense

Purpose: Cardiomyopathy is one of the major complications of {beta} thalassaemia major as a result of transfusion iron overload. The aim of our study is to evaluate with MR if there is any difference of iron deposition signal intensity (SI) or distribution between non-cardiopatic and cardiopatic thalassaemic patients in order to establish if there is a relationship between cardiopathy and iron deposition. Materials and methods: We studied 20 patients affected by {beta} thalassaemia major, of whom 10 cardiopatic and 10 non-cardiopatic, and 10 healthy volunteers as control group. Serum ferritin and left ventricular ejection fraction were calculated in thalassaemic patients. All patients were examinated using a 1.5 MR unit with ECG-gated GE cine-MR T2*-weighted, SE T1-weighted and GE T2*-weighted sequences. In all cases, using an adequate ROI, the myocardial and skeletal muscle signal intensity (SI), the myocardial/skeletal muscle signal intensity radio (SIR) and the SI average of the ...

2005-02-01

212

Viruses with More Than 1,000 Genes: Mamavirus, a New Acanthamoeba polyphaga mimivirus Strain, and Reannotation of Mimivirus Genes

Autophagy is a ubiquitous catabolic process that ensures organism’s well-being by sequestering a wide array of undesired intracellular constituents into double-membrane vesicles termed...Full Text Available

2010-12-01

213

Virological and serological surveillance for type A influenza in the black-legged kittiwake (Rissa tridactyla)

The genome sequence of the Mamavirus, a new Acanthamoeba polyphaga mimivirus strain, is reported. With 1,191,693 nt in length and 1,023 predicted protein-coding genes, the Mamavirus...Full Text Available

2011-01-01

214

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

BackgroundThe epidemiology of avian influenza viruses (AIVs) in gulls is only partially known. The role of the world's most numerous gull species, the black-legged kittiwake (Rissa...Full Text Available

215

Unusual Topological Arrangement of Structural Motifs in the Baboon Reovirus Fusion-Associated Small Transmembrane Protein

The diagnosis of viral causes of many infectious diseases is difficult due to the inherent sequence diversity of viruses as well as the ongoing emergence of novel viral pathogens, such as SARS coronavirus...Full Text Available

216

Translation Elongation Factor 1A Facilitates the Assembly of the Tombusvirus Replicase and Stimulates Minus-Strand Synthesis

Select members of the Reoviridae are the only nonenveloped viruses known to induce syncytium formation. The fusogenic orthoreoviruses accomplish cell-cell fusion through a distinct...Full Text Available

2005-05-01

217

Trans-activation of the JC virus late promoter by the tat protein of type 1 human immunodeficiency virus in glial cells

Replication of plus-strand RNA viruses depends on host factors that are recruited into viral replicase complexes. Previous studies showed that eukaryotic translation elongation factor (eEF1A) is one...Full Text Available

2010-11-01

218

Threats and countermeasures for network security

Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system caused by the JC virus (JCV), a human papovavirus. PML is a relatively rare disease seen predominantly in immunocompromised individuals and is a frequent complication observed in AIDS patients. The significantly higher incidence of PML in AIDS patients than in other immunosuppressive disorders has suggested that the presence of human immunodeficiency virus type 1 (HIV-1) in the brain may directly or indirectly contribute to the pathogenesis of this disease. In the present study the authors have examined the expression of the JCV genome in both glial and non-glial cells in the presence of HIV-1 regulatory proteins. They find that the HIV-1-encoded trans-regulatory protein tat increases the basal activity of the JCV late promoter, JCV{sub L}, in glial cells. They conclude that the presence of the HIV-1-encoded tat protein may positively ...

1990-05-01

219

The p14 FAST Protein of Reptilian Reovirus Increases Vesicular Stomatitis Virus Neuropathogenesis?

In the late 1980's, the traditional threat of anonymous break-ins to networked computers was joined by viruses and worms, multiplicative surrogates that carry out the bidding of their authors. Technologies for authentication and secrecy, supplemented by good management practices, are the principal countermeasures. Four articles on these subjects are presented.

1991-01-01

220

The Use of a Mobile Laboratory Unit in Support of Patient Management and Epidemiological Surveillance during the 2005 Marburg Outbreak in Angola

The fusogenic orthoreoviruses express nonstructural fusion-associated small transmembrane (FAST) proteins that induce cell-cell fusion and syncytium formation. It has been speculated that the FAST proteins...Full Text Available

2009-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

221

The Relationship between Leishmaniasis and AIDS: the Second 10 Years

BackgroundMarburg virus (MARV), a zoonotic pathogen causing severe hemorrhagic fever in man, has emerged in Angola resulting in the largest outbreak of Marburg hemorrhagic fever...Full Text Available

222

The Exceptionally Large Genome of Hendra Virus: Support for Creation of a New Genus within the Family Paramyxoviridae

Summary: To date, most Leishmania and human immunodeficiency virus (HIV) coinfection cases reported to WHO come from Southern Europe. Up to the year 2001, nearly 2,000 cases of coinfection were identified,...Full Text Available

2008-04-01

223

The Case for Selection at CCR5-?32

An outbreak of acute respiratory disease in Hendra, a suburb of Brisbane, Australia, in September 1994 resulted in the deaths of 14 racing horses and a horse trainer. The causative agent was a new member...Full Text Available

2000-11-01

224

Synergism among BIOLF-62, phosphonoformate, and other antiherpetic compounds.

The C-C chemokine receptor 5, 32 base-pair deletion (CCR5-Δ32) allele confers strong resistance to infection by the AIDS virus HIV. Previous studies have suggested...Full Text Available

2005-11-01

225

Syndecan-Fc Hybrid Molecule as a Potent In Vitro Microbicidal Anti-HIV-1 Agent?

9-[[2-Hydroxy-1-(hydroxymethyl)ethoxy]methyl]guanine (BIOLF-62) is highly synergistic with either phosphonoformate or phosphonoacetate when used in combination against herpes simplex virus types 1 and...Full Text Available

1982-12-01

226

Studies on the mechanism of 1,3-butadiene-induced leukemogenesis: the potential role of endogenous murine leukemia virus.

In the absence of a vaccine, there is an urgent need for the development of safe and effective topical microbicides to prevent the sexual transmission of human immunodeficiency virus type 1 (HIV-1)....Full Text Available

2010-07-01

227

Structural studies of peptides from HPVs (Human Papiloma Viruses) 9, 16 and 30 with LxCxE anchoring in the presence or absence of pRB (RetinoBlastoma protein)

Previous studies have revealed marked differences in the incidence of leukemia between rats and mice exposed to 1,3-butadiene that do not appear to be readily explained on the basis of pharmacokinetics...Full Text Available

1990-06-01

228

Strand displacement synthesis capability of Moloney murine leukemia virus reverse transcriptase.

... AUREMN), Rio de Janeiro, RJ (Brazil) [vp.] APPLIED LIFE SCIENCES cpb

2004-07-27

229

Sociocultural and epidemiological aspects of HIV/AIDS in Mozambique

The accepted model of retroviral reverse transcription includes a circular DNA intermediate which requires strand displacement synthesis for linearization and creation of an integration-competent, long...Full Text Available

1994-08-01

230

Silicates as Nonspecific Adsorbents of Bacteriophage: a Model for Purification of Water from Viruses

BackgroundA legacy of colonial rule coupled with a devastating 16-year civil war through 1992 left Mozambique economically impoverished just as the human immunodeficiency virus (HIV)...Full Text Available

231

Safety and immunogenicity of a vaccine bait containing ERA strain of attenuated rabies virus.

Amorphous silicates having small particles with a large surface area were found to have high nonspecific adsorption capacity. Investigations with Escherichia coli T4 bacteriophage have...Full Text Available

1980-01-01

232

Safety and Immunogenicity of Novel Recombinant BCG and Modified Vaccinia Virus Ankara Vaccines in Neonate Rhesus Macaques ?

Ninety percent of foxes fed commercial ERA vaccine in a specially designed bait developed rabies serum neutralizing antibodies. The vaccine bait did not cause clinical signs of rabies when consumed...Full Text Available

1987-10-01

233

Rifampin pharmacokinetics in children, with and without human immunodeficiency virus infection, hospitalized for the management of severe forms of tuberculosis

Although major inroads into making antiretroviral therapy available in resource-poor countries have been made, there is an urgent need for an effective vaccine administered shortly after birth, which...Full Text Available

2010-08-01

234

Recognition of historic influenza epidemics from parish burial records: a test of prediction from a new hypothesis of influenzal epidemiology.

BackgroundRifampin is a key drug in antituberculosis chemotherapy because it rapidly kills the majority of bacilli in tuberculosis lesions, prevents relapse and thus enables 6-month...Full Text Available

235

Rearrangement of a common cellular DNA domain on chromosome 4 in human primary liver tumors.

On the current conception of the epidemiology of epidemic influenza, as caused by a mechanism of direct spread of the virus from the sick, epidemics must have travelled much more slowly in former times...Full Text Available

1983-10-01

236

Passive immunity to feline leukemia: evaluation of immunity from dams naturally infected and experimentally vaccinated.

Hepatitis B virus (HBV) DNA integration has been shown to occur frequently in human hepatocellular carcinomas. We have investigated whether common cellular DNA domains might be rearranged, possibly...Full Text Available

1988-02-01

237

Paracetamol reduces influenza-induced immunopathology in a mouse model of infection without compromising virus clearance or the generation of protective immunity

Antibodies against feline leukemia virus (FeLV) and the feline oncornavirus-associated cell membrane antigen (FOCMA) were transferred from pregnant cats to their suckling kittens. All of these kittens...Full Text Available

1977-04-01

238

BackgroundSeasonal influenza A infection affects a significant cohort of the global population annually, resulting in considerable morbidity and mortality. Therapeutic strategies...Full Text Available

2011-05-01

239

On the defence notion

Nucleocapsid and Matrix Protein Contributions to Selective Human Immunodeficiency Virus Type 1 Genomic RNA Packaging

'Trojan horses', 'logic bombs', 'armoured viruses' and 'cryptovirology' are terms recalling war gears. In fact, concepts of attack and defence drive the world of computer virology, which looks like a war universe in an information society. This war has several shapes, from invasions of a network by worms, to military and industrial espionage ...

2007-01-01

240

Novel Approach for Differential Diagnosis of HIV Infections in the Face of Vaccine-Generated Antibodies

The nucleocapsid protein (NC) of retroviruses plays a major role in genomic RNA packaging, and some evidence has implicated the matrix protein (MA) of certain retroviruses in viral RNA binding. To further...Full Text Available

1998-03-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

241

Network security defence methods in IHEP

SummaryBecause increasing numbers of HIV vaccine candidates are being tested globally, it is essential to differentiate vaccine-from virus-induced antibodies. Most of the currently...Full Text Available

2006-11-01

242

Neoplastic transformation of prostatic and urogenital epithelium by the polyoma virus middle T gene.

This article is about the network security defence method and technique at IHEP. Including: the experience, research result and application in network outlet security, server security, local network security, network security monitoring and collecting evidence, anti-virus etc

2002-12-01

243

Naturally occurring double-stranded RNA and immune responses. Effects on plaque-forming cells and antibody formation.

Male transgenic mice expressing the polyomavirus middle T (PyV-MT) gene exhibited growth and developmental abnormalities in prostatic and other urogenital epithelium. Expression of PyV-MT was directed...Full Text Available

1996-10-01

244

Modulation of B-cell endoplasmic reticulum calcium homeostasis by Epstein-Barr virus Latent Membrane Protein-1

A highly purified preparation of double-stranded RNA, obtained from virus-like particles in Penicillium cultures, was found to affert humoral immune responses in mice differentially depending on its...Full Text Available

1975-03-01

245

Misdiagnosed HIV infection in pregnant women: implications for clinical care.

BackgroundCalcium signaling plays an important role in B lymphocyte survival and activation, and is critically dependent on the inositol-1,4,5-tris-phosphate-induced...Full Text Available

246

Minigenome-Based Reporter System Suitable for High-Throughput Screening of Compounds Able To Inhibit Ebolavirus Replication and/or Transcription ?

Out of nearly 900 women in a research study of human immunodeficiency virus infection in pregnancy, 8 were subsequently found not to be infected. Misdiagnoses could have resulted from (a) laboratory...Full Text Available

1994-09-01

247

Memories of John N. Brady: scientist, mentor and friend

We describe an Ebolavirus minigenome-based system that is suitable for high-throughput screening of compounds able to impair Ebolavirus virus replication and/or transcription....Full Text Available

2010-07-01

248

Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human

Friends and colleagues remember John N. Brady, Ph.D., Chief of the Virus Tumor Biology Section of the Laboratory of Cellular Oncology, who died much too young at the age of 57 on April 27, 2009 of colon...Full Text Available

249

Mechanism of Host Defense Suppression Induced by Viral Infection: Mode of Action of Inosiplex as an Antiviral Agent

In 1871, the observation of yellowish nodules in the enlarged spleen of a cow was considered to be the first reported case of bovine leukemia. The etiological agent of this lymphoproliferative disease,...Full Text Available

250

Long-term impact of acyclovir suppressive therapy on genital and plasma HIV RNA in Tanzanian women: a randomised controlled trial

The mechanism of influenza virus (INFV)-induced immunosuppression and the mode of inosiplex action against INFV infection were studied. INFV suppressed both anti-lipopolysaccharide and anti-sheep erythrocyte...Full Text Available

1982-10-01

251

Lnk controls mouse hematopoietic stem cell self-renewal and quiescence through direct interactions with JAK2

BackgroundHerpes simplex virus (HSV) suppressive therapy reduces genital and plasma HIV-1 RNA over periods up to three months but the longer-term effect is unknown.Full Text Available

2010-05-01

252

Lessons in Signaling and Tumorigenesis from Polyomavirus Middle T Antigen

In addition to its role in megakaryocyte production, signaling initiated by thrombopoietin (TPO) activation of its receptor, myeloproliferative leukemia virus protooncogene (c-Mpl, or Mpl), controls...Full Text Available

2008-08-01

253

Lentiviral vector-mediated stable expression of sTNFR-Fc in human macrophage and neuronal cells as a potential therapy for neuroAIDS

Summary: The small DNA tumor viruses have provided a very long-lived source of insights into many aspects of the life cycle of eukaryotic cells. In recent years, the emphasis has been on cancer-related...Full Text Available

2009-09-01

254

Involvement of the Matrix and Nucleocapsid Domains of the Bovine Leukemia Virus Gag Polyprotein Precursor in Viral RNA Packaging

BackgroundHuman immunodeficiency virus type 1 (HIV-1) infection frequently causes neurologic disease, which is the result of viral replication and activation of macrophages and microglia...Full Text Available

255

Inactivation and Heat Stabilization of Poliovirus by 2-Thiouracil ¹

The RNA packaging process for retroviruses involves a recognition event of the genome-length viral RNA by the viral Gag polyprotein precursor (PrGag), an important step in particle morphogenesis. The...Full Text Available

2003-09-01

256

In vivo hypermutation of xenotropic murine leukemia virus-related virus DNA in peripheral blood mononuclear cells of rhesus macaque by APOBEC3 proteins.

Treatment of poliovirus Type I with 10⁻³m 2-thiouracil (2-TU) resulted in the inactivation of greater than 90% of the virus infectivity and stabilization of approximately...Full Text Available

1967-08-01

257

Human spumaretrovirus-related sequences in the DNA of leukocytes from patients with Graves disease.

The gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV), replicates to high titers in some human cell lines and is able to infect non-human primates. To determine whether APOBEC3 (A3) proteins restrict XMRV infections in a non-human primate model, we sequenced proviral DNA from peripheral blood mononuclear cells of XMRV-infected rhesus macaques. Hypermutation characteristic of A3DE, A3F and A3G activities was observed in the XMRV proviral sequences in vivo. Furthermore, expression of rhesus A3DE, A3F, or A3G in human cells inhibited XMRV infection and caused hypermutation of XMRV DNA. These studies show that some rhesus A3 isoforms are highly effective against XMRV in the blood of a non-human primate model of infection and in cultured human cells. PMID:21982221

2011-10-01

258

Human Immunodeficiency Virus type-1 reverse transcriptase exists as post-translationally modified forms in virions and cells

Viruses, and more particularly retroviruses, have been postulated to play a role in the pathogenesis of autoimmune diseases. In a search for spumaretrovirus infection markers, we screened a group of...Full Text Available

1992-11-01

259

High-Sensitivity Analysis of Naturally Occurring Sugar Chains, Using a Novel Fluorescent Linker Molecule

BackgroundHIV-1 reverse transcriptase (RT) is a heterodimer composed of p66 and p51 subunits and is responsible for reverse transcription of the viral RNA genome into DNA. RT can...Full Text Available

260

HIV/AIDS Counseling Skills and Strategies: Can Testing and Counseling Curb the Epidemic?

To analyse the binding of sugar chains to proteins, viruses and cells, the surface plasmon resonance (SPR) technique is very convenient and effective because it is a real-time, non-destructive detection...Full Text Available

2009-07-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

261

HIV prevention in prisons and jails: obstacles and opportunities.

Objectives:The human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) epidemic is in its third decade and has reached to alarming proportions worldwide....Full Text Available

2011-01-01

262

Genomic Expression Libraries for the Identification of Cross-Reactive Orthopoxvirus Antigens

High rates of human immunodeficiency virus (HIV) infection among jail and prison inmates suggest that HIV prevention efforts should focus on incarcerated populations. Overcrowding, the high prevalence...Full Text Available

1994-09-01

263

Followup study of possible HIV seropositivity among abusers of parenteral drugs in 1971-72.

Increasing numbers of human cowpox virus infections that are being observed and that particularly affect young non-vaccinated persons have renewed interest in this zoonotic disease. Usually causing...Full Text Available

264

Experimental avian paramyxovirus serotype-3 infection in chickens and turkeys

Serum specimens obtained from a nationwide sample of parenteral drug abusers (PDAs) during the period 1971-72 had previously been screened for human immunodeficiency virus (HIV) antibodies. Some specimens...Full Text Available

1991-07-01

265

Epigallocatechin gallate inhibits HBV DNA synthesis in a viral replication - inducible cell line

Avian paramyxoviruses (APMV) are divided into nine serotypes. Newcastle disease virus (APMV-1) is the most extensively characterized, while relatively little information is available for the other APMV...Full Text Available

2010-09-01

266

Encephalitis lethargica and the influenza virus. III. The influenza pandemic of 1918/19 and encephalitis lethargica: neuropathology and discussion*

AIM: To analyze the antiviral mechanism of Epigallocatechin gallate (EGCG) against hepatitis B virus (HBV) replication.METHODS: In this research, the HBV-replicating cell line HepG2.117 was used...Full Text Available

2011-03-21

267

Emulsified Nanoparticles Containing Inactivated Influenza Virus and CpG Oligodeoxynucleotides Critically Influences the Host Immune Responses in Mice

This is the second of two papers which critically examine the relationship between the 1918/19 influenza pandemic and encephalitis lethargica (EL). The role of influenza in the etiology of EL...Full Text Available

2009-10-01

268

Effect of 9-(2-Hydroxyethoxymethyl)guanine on Herpesvirus-Induced Keratitis and Iritis in Rabbits

BackgroundAntigen sparing and cross-protective immunity are regarded as crucial in pandemic influenza vaccine development. Both targets can be achieved by adjuvantation strategy...Full Text Available

269

Determination of antibody response to influenza virus surface glycoproteins by kinetic enzyme-linked immunosorbent assay.

Drugs used for the inhibition of DNA viruses, such as iododeoxyuridine, adenine arabinoside, or trifluorothymidine, are not biochemically selective in their action and also interfere with normal cellular...Full Text Available

1978-12-01

270

Determinants of antiviral treatment initiation in a hepatitis C-infected population benefiting from universal health care coverage

We modified an existing enzyme-linked immunosorbent assay (ELISA) to be able to use new spectrophotometers which can measure the rate of color development in microtiter wells. This new kinetic-based...Full Text Available

1988-10-01

271

Detecting Retroviral Sequences in Chronic Fatigue Syndrome

BACKGROUND AND AIMS:In view of increasing therapeutic efficacy, the delivery of hepatitis C virus (HCV) antiviral treatment is expected to increase. Yet practical experience reveals...Full Text Available

2007-06-01

272

Deformed Wing Virus Implicated in Overwintering Honeybee Colony Losses ?

XMRV or xenotropic murine leukemia virus-related retrovirus, a recently discovered retrovirus, has been linked to both prostate cancer and chronic fatigue syndrome (CFS). Recently, the teams of Drs....Full Text Available

273

Defining and measuring the costs of the HIV epidemic to business firms.

The worldwide decline in honeybee colonies during the past 50 years has often been linked to the spread of the parasitic mite Varroa destructor and its interaction with certain honeybee...Full Text Available

2009-11-01

274

Complexes of hepatitis B surface antigen and immunoglobulin M in the sera of patients with hepatitis B virus infection.

Most published estimates of the costs of the epidemic of human immunodeficiency virus (HIV) infection and acquired immunodeficiency syndrome (AIDS) have been developed from the societal perspective,...Full Text Available

1994-05-01

275

Comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon

Hepatitis B surface antigen (HBsAg) bound to immunoglobulin M (IgM) was detected in sera of HBsAg carriers by a radioimmunoassay based on selective absorption of the immunoglobulin on a solid phase...Full Text Available

1983-09-01

276

Coat protein gene duplication in a filamentous RNA virus of plants.

BackgroundWhite spot syndrome (WSS) is a viral disease that affects most of the commercially important shrimps and causes serious economic losses to the shrimp farming industry worldwide....Full Text Available

277

Breaking the 1000-gene barrier for Mimivirus using ultra-deep genome and transcriptome sequencing

Computer-assisted analysis revealed a striking sequence similarity between the putative 24-kDa protein (p24) encoded by open reading frame (ORF) 5 of beet yellows closterovirus and the coat protein...Full Text Available

1992-10-01

278

Breakdown of the Blood-Brain Barrier during Tick-Borne Encephalitis in Mice Is Not Dependent on CD8⁺ T-Cells

BackgroundMimivirus, a giant dsDNA virus infecting Acanthamoeba, is the prototype of the mimiviridae family, the latest addition to the family of the nucleocytoplasmic...Full Text Available

279

Bone Disease in HIV Infection: A Practical Review and Recommendations for HIV Care Providers

Tick-borne encephalitis (TBE) virus causes severe encephalitis with serious sequelae in humans. The disease is characterized by fever and debilitating encephalitis that can progress to chronic illness...Full Text Available

280

Activation of Cytotoxic and Regulatory Functions of NK Cells by Sindbis Viral Vectors

Low bone mineral density (BMD) is prevalent in human immunodeficiency virus (HIV)–infected subjects. Initiation of antiretroviral therapy is associated with a 2%–6% decrease...Full Text Available

2010-10-15

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

281

A versatile and potentially general approach to the targeting of specific cell types by retroviruses: Application to the infection of human cells by means of major histocompatibility complex class I and class II antigens by mouse ecotropic murine leukemia virus-derived viruses

Oncolytic viruses (OVs) represent a relatively novel anti-cancer modality. Like other new cancer treatments, effective OV therapy will likely require combination with conventional treatments. In order...Full Text Available

282

A molecular epidemiological study of rabies epizootics in kudu (Tragelaphus strepsiceros) in Namibia

A technique for delivering genes carried by recombinant retroviruses into specific cell types could have numerous applications in oncology, developmental biology, and gene therapy. As a first step toward this remote goal the authors designed a procedure allowing in vitro cell targeting by retroviruses. Biotinylated antibodies against the viral envelope protein on one side, and against specific cell membrane markers on the other side, were bridged by streptavidin and used to link the virus to the host. The method was successfully used to infect human cells with ecotropic murine retroviruses by means of major histocompatibility complex class I and class II antigens and appears easily adaptable to other cell, membrane markers. Moreover, the sequential protocol they design, although allowing infection of human cells, requires less stringent safety constraints than would handling of amphotropic virus stocks.

1989-12-01

283

The wavelength dependence of ultraviolet enhanced reactivation in a mammalian cell-virus system

BackgroundA panel of 37 rabies virus isolates were collected and studied, originating mainly from the northern and central regions of Namibia, between 1980 and 2003.ResultsThese...Full Text Available

284

Magnetic silica extraction for low-viremia human immunodeficiency virus type 1 genotyping.

The effect of UV radiation in the wavelength region 230 nm to 302 nm on the ability of an irradiated mammalian cell to reactivate UV-irradiated mammalian virus was tested. An action spectrum for radiation enhanced reactivation (RER) is presented. The shape of the action spectrum points to a combined nucleic acid-protein target for UV radiation effects on this cellular parameter. An analysis of the results of others involving the biochemical and photobiological events involved in RER does not allow us to distinguish which macromolecule is the major contributor to this effect. Studies involving an analogous phenomenon in bacteris (Weigle reactivation) imply that RER and WR amy involve similar mechanisms. (author).

285

The carrying out process of Kyoto Protocol in Italy. Methods, scenarios and policy evaluation; Il processo di attuazione del Protocollo di Kyoto in Italia. Metodi, scenari e valutazione di politiche e misure

Nucleic acid extraction and human immunodeficiency virus type 1 (HIV-1) genotyping using the NucliSens miniMAG platform and the TruGene HIV-1 genotyping kit gave HIV-1 sequence data from HIV-1-negative plasma spiked with 100 copies/ml reference HIV-1 RNA and from low-viremia clinical samples (<500 copies/ml) without the need for ultracentrifugation or nested second-round PCR. PMID:17122019

2006-11-22

286

Mesenchymal tumors of the pancreas. CT patterns; Tumori mesenchimali del pancreas: aspetti con tomografia computerizzata

This paper reports some technical contributes related to the Kyoto Protocol and its application in Italian decision making policy. [Italian] Il presente volume raccoglie una decina di contributi tecnici originariamente preparati o commissionati dall'ENEA, Ente Nazionale per le Nuove Tecnologie, l'Energia e l'Ambiente, in vista della Conferenza Nazionale Energia e Ambiente. Questa fotografia di una situazione in rapida evoluzione e' stata preparata da esperti del settore che contribuiscono a redigere il materiale tecnico di base utilizzato dai negoziatori nazionali durante le trattative internazionali e dai responsabili nazionali per avviare il processo di presa di decisioni in materia.

2000-07-01

287

Liquid and gaseous effluent control and monitoring at Cernavoda NPP and the assessment of the environmental impact

Purpose of this item is to report the CT patterns of pancreatic mesenchymal lesions and to investigate the capabilities and limitations of this technique in terms of characterization. In selected cases, CT can provide virtually diagnostic information (lipoma and teratoma), or at least highly suggestive findings (NHL, plexiform neurofibroma, lymphangioma). Moreover, knowledge of the variable morphostructural patterns of mesenchymal hisototypes (SCH, Sarcoma etc.) permits to include them in the differential diagnosis of pancreatic masses. [Italian] Scopo di questo rapporto e' descrivere gli aspetti semeiologici dei tumori mesenchimali del pancreas con la TC verificandone le possibilita e i limiti per la caratterizzazione. Pur nella rarita' dei reperti considerati, la TC puo' consentire l'effettiva caratterizzazione in alcuni casi (lipoma, neurofibroma plessiforme, linfangioma) o includere anche istotipi meno comuni (Schwannoma, ...

1999-10-01

288

Interleukin-8 Expression in Lung Tissue During Ischemia-Reperfusion

The release of any potential radioactive pollutant to the environment during routine operation of a Nuclear Power Plant should be the subject of appropriate controls and assessments. It is impossible to monitor directly the dose contribution of normal releases because the environmental radioactivity levels are very small but source monitoring provides a means of assessing the radiation exposure of population groups, critical groups and individual members of the public. Derived emissions limits ( DELs ) are used to quantify the relationship between releases of radioactivity and doses to public - critical groups. CNE Cernavoda DELs are based on a pathway analysis conducted for Cernavoda site specific conditions and they were computed using a compartment transfer model. Annual air and water emissions for the most significant radionuclides between 1997 and 2008 are presented in terms of doses and can be observed that population doses are far below ...

2009-10-12

289

British Library Electronic Table of Contents (United Kingdom)

Objetivo La generacion local de citocinas es un factor patogenico en el dano por isquemia-reperfusion en la disfuncion precoz del injerto. Este estudio analiza la expresion en tejido pulmonar de ARN mensajero de interleucina-8 (ARNm de IL-8) y su relacion con los cambios intersticiales pulmonares en un modelo experimental de isquemia-reperfusion pulmonar normotermica. MATERIAL Y METODOS Se estudiaron 16 conejos de la raza Nueva Zelanda en 3 grupos de estudio: a) basal; b) isquemia (lobulo inferior derecho tras isquemia de 1, 2 o 3 h), y c) reperfusion (lobulos superior y medio derechos tras 1 h de isquemia y 1, 2 o 3 h de reperfusion). Se deter- mino la expresion del ARNm de IL-8 mediante transcripcion inversa y reaccion en cadena de la polimerasa y estudio la infiltracion intersticial por...

2007-01-01

290

Development of a new tool for predicting water quality. Verification on a stretch of the river Ebro; Desarrollo de una nueva herramienta para la prediccion de la calidad del agua. Verificacion en un tramo del rio Ebro

Determination of seepage path in a river levee; Sulla determinazione della linea d'infiltrazione al'interno di un argine fluviale

The Water Frame work Directive (December 2000) proposes integrated water management in regard to both quality and quality. A mathematical model has therefore been produced at the CEIT and EPTISA research centre, in collaboration with the Ebro Hydrographic. Confederation (CHE), to predict water quality and thus begin integrated management of it in the river basins. A description is given of the characteristics of the simulation tool. The hydraulic model is based on the numerical (weighted, four-point implicit) resolution of the complete Saint Venant equations. The quality model is based on the IWA River Water Quality Model Number 1 which has clear advantages (consistency, mass balance and easy integration with the biological models of the waste water treatment plants and the collector networks compared to traditional models. The experimental validation, which includes calibration in wintertime of the hydraulic and quality models most representative coefficients was assessed on the basis ...

2004-07-01

291

Color Doppler sonography in the study of chronic ischemic nephropathy

After a short review of the classical simplified expressions able to evaluate the seepage path in the core of a river levee, this paper first discusses the results obtained assuming the Dupuit approximation. Dimensionless nomographs are then showed, obtained by means of numerical integration of the seepage equations, These nomographs allow a quick levee design to be developed, after the definition of flood hydrography, seepage and embankment characteristics. [Italian] Dopo un sintetico richiamo di alcune classiche formulazioni semplificate per la determinazione dell'andamento della linea di infiltrazioner in un rilevato arginale, nell'articolo vengono presentati e discussi i risultati conseguiti prima assumendo la sola ipotesi di Dupuit e, successivamente, risolvendo l'equazione completa del moto di filtrazione. In particolare, vengono forniti abachi adimensionali che consentono, una volta fissate le caratteristiche ...

1999-10-01

292

British Library Electronic Table of Contents (United Kingdom)

SommarioNei Paesi occidentali il rischio di malattie cardiovascolari e notevolmente aumentato negli ultimi decenni. Di pari passo, il miglioramento prognostico delle malattie cardiovascolari, l'invecchiamento e l'aumento della vita media della popolazione generale stanno rivelando un crescente numero di casi di malattia renale cronica d'origine vascolare. E del tutto plausibile pensare che nei prossimi anni il numero di pazienti anziani con malattia vascolare aterosclerotica, che sara inserito nei programmi di dialisi cronica, aumentera rapidamente. Questo fara lievitare in modo drammatico i costi sociali ed economici della dialisi configurando una vera emergenza clinica. In questo scenario epidemiologico, la diagnosi precoce della malattia ischemica cronica del rene e la definizione di nu...

2008-01-01

293

Abdominal adipose tissue distribution in obese children. US versus CT measurements; Valutazione della distribuzione del tessuto adiposo addominale nei bambini obesi. Confronto tra ecografia e Tomografia Computerizzata

Vascular injuries in intestinal obstruction. The role of computed tomography; Complicazioni vascolari nelle occlusioni intestinali: ruolo della tomografia computerizzata

Computed Tomography (CT) and, more recently, ultrasound (US), have proved excellent tools for quantifying adipose tissue distribution. Body fat distribution is an important factor in the treatment of obesity and its complications. In this work it is investigated the correlation between CT and US measurements in pediatric obesity. Forty obese children and adolescents aged 4.1-14.8 years were submitted to CT and US. Intra-abdominal, subcutaneous and total body fat were calculated (in cm{sup 2}), with the CT image analysis software. The rectus muscle-spine and rectus muscle-aorta distances, as indicative of visceral fat thickness, were measured on US images with(out) compression. The distance between skin fat and fat-rectus muscle interfaces was measured as subcutaneous fat thickness. US-CT findings have been compared with other morphometric variables-i.e., patient's (ideal) body weight and skin fold measures. A statistically significant correlation was found between the CT ...

1999-12-01

294

Suburban amoebiasis. CT and US findings and percutaneous treatment of amoebic liver abscess; Amebiasi sub-urbana: aspetti diagnostici con tomografia computerizzata ed ecografia e trattamento percutaneo degli ascessi amebici del fegato

The paper investigates computerized tomography capabilities in showing vascular complications secondary to intestinal obstruction and for assessing the site and cause of obstruction. [Italian] Scopo del lavoro e' lo studio dell'uso della tomografia computerizzata nel riconoscimento di complicanze vascolari secondarie ad occlusione intestinale e della presenza, della natura e della sede dell'ostacolo alla canalizzazione intestinale.

1999-09-01

295

Gunshot wounds of abdomen detected at CT. Personal experience in 30 patients; Ferite da arma da fuoco dell'addome studiate con tomografia computerizzata: esperienza personale in 30 casi

The study reports ultrasonography and computerise tomography findings in 16 patients with amoebic abscesses, 12 of whom lived in a temperate peripheral area north-east of Naples (Italy). All patients have a clinical-diagnostic condition called sub-urban amoebiasis. The personal experience with the US guided therapeutic drainage of amoebic abscess with repeated cavity washing, which is important for positive parasitology. Combined US and CT assessment facilitated the diagnosis of amoebiasis and its differentiation from pyogenic abscess and hepatoma. [Italian] La colonizzazione epatica rappresenta la localizzazione piu' comune dell'amebiasi extraintestinale e gli ascessi epatici ne costituiscono la manifestazione piu' frequente sviluppandosi nel 3-9 % dei pazienti affetti da infezione parassitaria. Diversi studi confermano che la terapia medica dell'amebiasi risulta piu' efficace quando viene associato il drenaggio percutaneo degli ascessi ...

2000-03-01

296

Bone geometry, biomechanics, and mineral content of the distal radius in uremic patients with severe hyperparathyroidism; Valutazione delle proprieta' geometriche, biomeccaniche e osteodensitometriche del radio ultradistale mediante tomografia computerizzata quantitativa periferica nei pazienti uremici affetti da iperparatiroidismo grave

The paper investigates the yield and the role of the computerized tomography in diagnosing abdominal gunshot wounds, with their rich and varied radiological signs and associated injuries, and subsequently used in the diagnosis. [Italian] Scopo del lavoro e' riportare l'esperienza personale nell'uso della tomografia computerizzata per riconoscere le lesioni da arma da fuoco dell'addome e nelle fasi successive dell'approfondimento diagnostico.

1999-09-01

297

The microzooplankton and its distribution in different aquatic environment; Il microzooplancton e la sua distribuzione nei diversi ambienti

Bone integrity and mineral status were studied with a noninvasive method in uremic patients with severe secondary hyperparathyroidism undergoing maintenance hemodialysis. [Italian] Gli autori propongono un metodo non invasivo per studiare la densita' minerale e le caratteristiche geometriche e biomeccaniche dell'osso nei pazienti uremici affetti da iperparatiroidismo secondario grave.

1999-04-01

298

Environmental management; La gestione ambientale

In this work some examples of studies performed with the use of microzooplankton as biological indicator are reported. [Italian] Scopo del lavoro e' quello di dimostrare come lo studio della distribuzione del microzooplancton in ambienti aquatici, possa permettere la caratterizzazione trofica dell'ambiente oggetto di studio. Il microzooplancton, infatti, essendo un componente fondamentale della catena trofica di differenti ambienti acquatici, puo' essere considerato un buon {sup i}ndicatore biologico{sup .} E' quindi riportata un'ampia ed approfondita analisi sistematica, necessaria al fine di individuarne le diverse specie dalla cui presenza (oltreche' dal numero degli esemplari) e' possibile desumere lo stato chimico-fisico e trofico di un determinato ambiente acquatico. Sono poi indicati i diversi metodi per il campionamento, la conservazione, la concentrazione ed il conteggio ...

1999-07-01

299

Aarskog-Scott syndrome: Clinical update and report of nine novel mutations of the FGD1 gene

Nowadays, unlike in the past, companies have to operate in a context of sustainable development, in which the economic and social development, production and consumption have to take into account the medium and long term impact on environment. The article sets forth some considerations about these subjects, which are assuming a growing importance in the management of companies: the variable environment may for instance be a factor of discrimination between being competitive or not. In order to characterise the context within which the environmental management has to be applied, some basic concepts about environmental management systems, Life Cycle Assessment, and Eco labeling are illustrated. As an example of application of the methodology described, a brief reference to the Italgas Group Environmental Report is given. [Italian] Oggi, a differenza del passato, le imprese si trovano ad operare in un contesto di sviluppo sostenibile, nel quale l'evoluzione ...

2000-04-01

300

CT assessment of blunt diaphragmatic injuries. A retrospective study on 35 cases; Sensibilita' diagnostica della Tomografia Computerizzata nei traumi chiusi del diaframma. Studio retrospettivo di 35 casi consecutivi

Mutations in the FGD1 gene have been shown to cause Aarskog-Scott syndrome (AAS), or facio-digito-genital dysplasia (OMIM#305400), an X-linked disorder characterized by distinctive genital and skeletal developmental abnormalities with a broad spectrum of clinical phenotypes. To date, 20 distinct mutations have been reported, but little phenotypic data are available on patients with molecularly confirmed AAS. In the present study, we report on our experience of screening for mutations in the FGD1 gene in a cohort of 60 European patients with a clinically suspected diagnosis of AAS. We identified nine novel mutations in 11 patients (detection rate of 18.33%), including three missense mutations (p.R402Q; p.S558W; p.K748E), four truncating mutations (p.Y530X; p.R656X; c.806delC; c.1620delC), one in-frame deletion (c.2020_2022delGAG) and the first reported splice site mutation (c.1935+3A>C). A recurrent mutation (p.R656X) was ...

2010-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

301

Production of Infectious Genotype 1b Virus Particles in Cell Culture and Impairment by Replication Enhancing Mutations

Purpose of this work is to evaluate the effectiveness and role of CT in blunt diaphragmatic injuries by reviewing our 8-year experience. It is reviewed the preoperative CT findings of 35 patients with surgically confirmed diaphragmatic rupture. Surgical repair was performed in the acute setting (within 12 hours of trauma) in 22 cases, and late (8 months-5 years) in 13 cases. Twenty-eight patients (80%) were examined with conventional CT and 7 (20%) with Helical CT. Of the 13 patients examined long after trauma, the left hemi diaphragm was ruptured in 12 cases (with visceral herniation in 4), and the right hemi diaphragm in 1, with no herniation. The diaphragmatic rent was found in the dome (15 cases, 43%), muscolotendinous junction (11 cases, 31%), muscular portion (8 cases, 23%), and at the muscular attachments on the ribs (1 case, 3%). As for the site of diaphragmatic injury, CT never depicted the diaphragmatic rent in the dome and at the muscolotendinous junction (74%), not even ...

2000-02-01

302

Histopathological and immunohistochemical findings of 20 autopsy cases with 2009 H1N1 virus infection.

With the advent of subgenomic hepatitis C virus (HCV) replicons, studies of the intracellular steps of the viral replication cycle became possible. These RNAs are capable of self-amplification in cultured human hepatoma cells, but save for the genotype 2a isolate JFH-1, efficient replication of these HCV RNAs requires replication enhancing mutations (REMs), previously also called cell culture adaptive mutations. These mutations cluster primarily in the central region of non-structural protein 5A (NS5A), but may also reside in the NS3 helicase domain or at a distinct position in NS4B. Most efficient replication has been achieved by combining REMs residing in NS3 with distinct REMs located in NS4B or NS5A. However, in spite of efficient replication of HCV genomes containing such mutations, they do not support production of infectious virus particles. By using the genotype 1b isolate Con1, in this study we show that REMs interfere with HCV ...

2009-06-12

303

Characterization of monocyte differentiation-inducing (MDI) factors derived from human fetal membrane chorion cells undergoing apoptosis after influenza virus infection.

Twenty autopsy cases with 2009 pandemic influenza A (2009 H1N1) virus infection, performed between August 2009 and February 2010, were histopathologically analyzed. Hematoxylin-eosin staining, immunohistochemistry for type A influenza nucleoprotein antigen, and real-time reverse transcription-PCR assay for viral RNA were performed on formalin-fixed and paraffin-embedded specimens. In addition, the D222G amino acid substitution in influenza virus hemagglutinin, which binds to specific cell receptors, was analyzed in formalin-fixed and paraffin-embedded trachea and lung sections by direct sequencing of PCR-amplified products. There were several histopathological patterns in the lung according to the most remarkable findings in each case: acute diffuse alveolar damage (DAD) with a hyaline membrane (four cases), organized DAD (one case), acute massive intra-alveolar edema with variable degrees of hemorrhage (three cases), neutrophilic ...

2011-08-26

304

Liver trauma from penetrating injuries. Miscellanea, personal series, clinical and CT findings; Traumi epatici da lesioni penetranti. Miscellanea, casistica personale, aspetti clinici e con Tomografia Computerizzata

Influenza virus infection during pregnancy has been implicated as one of cause of premature delivery, abortion and stillbirth. We have reported that cultured human fetal membrane chorion cells undergoing apoptosis by influenza virus infection secrete unidentified heat-stable monocyte differentiation-inducing (MDI) factors. In this study, cellular, biological and immunochemical characteristics of MDI factors were investigated using human monocytic leukemia THP-1 cells by nitroblue tetrazolium reduction and cell adhesion assays. The treatment of THP-1 cells with culture supernatants from the influenza virus-infected chorion cells induced the nitroblue tetrazolium reduction ability, which was inhibited by the addition of superoxide dismutase and diphenyleneiodonium chloride, an inhibitor for reduced nicotinamide adenine dinucleotide phosphate oxidase. The phenomenon was also observed in human peripheral blood monocytes and ...

2006-06-06

305

Replication-defective vectors of reticuloendotheliosis virus transduce exogenous genes into somatic stem cells of the unincubated chicken embryo

Penetrating liver wounds are related to many causes and rank second after blunt abdominal and liver trauma. In this report are examined the clinical and radiological findings of personal series of patients with penetrating trauma, especially by firearms and stab and cut wounds. It will also tried to define the diagnostic workup of these traumas, which is especially based on CT signs of liver damage and associated changes and which is of basic importance for following treatment, both surgical or conservative. In the last seven years it was retrospectively reviewed 31 cases of penetrating liver trauma. The patients were 19 men and 12 women, ranging in age 18 to 73 (mean 42), with penetrating liver injuries from firearms (16 patients) and stab (9 cases) wounds; 6 patients had injuries from different cases. Abdominal CT was carried out in emergency with the CT Angiography (CTA) technique in all patients. In the patients with suspected chest and abdomen involvement CT was performed from the ...

2000-12-01

306

Human T-cell lymphotropic virus type III infection of the central nervous system: a preliminary in situ analysis

Replication-defective vectors derived from reticuloendotheliosis virus were used to transduce exogenous genes into early somatic stem cells of the chicken embryo. One of these vectors transduced and expressed the chicken growth hormone coding sequence. The helper cell line, C3, was used to generate stocks of vector containing about 10/sup 4/ transducing units per ml. Injection of 5- to 20-..mu..l volumes of vector directly beneath the blastoderm of unincubated chicken embryos led to infection of somatic stem cells. Infected embryos and adults contained unrearranged integrated proviral DNAs. Embryos expressed the transduced chicken growth hormone gene and contained high levels of serum growth hormone. Blood, brain, muscle, testis, and semen contained from individuals injected as embryos contained vector DNA. Replication-defective vectors of the reticuloendotheliosis virus transduced exogenous genes into chicken embryonic stem cells in vivo.

1989-06-01

307

Current status of a hepatitis C vaccine: encouraging results but significant challenges ahead

Patients with acquired immunodeficiency syndrome (AIDS) are subject to a spectrum of central nervous system (CNS) disorders. Recent evidence implicates the human T-cell lymphotropic virus type III (HTLV-III) in the pathogenesis of some of these illnesses, although the cells infected by the virus have yet to be identified. Using in situ hybridization, the authors examined brain tissue from two patients with AIDS encephalopathy for the presence of HTLV-III RNA. In both cases, viral RNA was detected and concentrated in, though not limited to, the white matter. The CNS cells most frequently infected included macrophages, pleomorphic microglia, and multinucleated giant cells. Less frequently, cells morphologically consistent with astrocytes, oligodendroglia, and rarely neurons were also infected. The findings strengthen the association of HTLV-III with the pathogenesis of AIDS encephalopathy. In situ hybridization can be applied to routinely ...

1986-11-07

308

Characteristic findings of pediatric inpatients with pandemic (H1N1) 2009 virus infection among severe and nonsevere illnesses

Persistent hepatitis C virus (HCV) infection affects 170 million people worldwide. Acute HCV infection is often asymptomatic, but many infected individuals develop persistent infections that may lead to development of end-stage liver diseases, including liver cirrhosis and hepatocellular carcinoma. Thus, an HCV vaccine that could significantly lower the chronicity rate would have a major impact on the disease burden. Unfortunately, HCV is a highly mutable virus, and escape mutations can undermine vaccine-induced virus-specific immunity. Also, HCV exists as multiple genotypes, and so genotype-specific vaccines might be required to achieve broad protection. Finally, vaccine development has been hampered by the lack of a small animal model and cell culture systems, but these are currently being established. Despite these obstacles, several vaccine candidates tested in the chimpanzee HCV model have shown some encouraging ...

2007-01-01

309

British Library Electronic Table of Contents (United Kingdom)

We analyzed the clinical features of inpatients at a Japanese pediatric department who were infected with pandemic (H1N1) 2009 virus. Study participants included 46 children hospitalized from July 2009 to January 2010. Infection with the virus was confirmed using real-time reverse transcriptase polymerase chain reaction (RT-PCR). The epidemic month was October 2009; 34 patients were boys, and median age was 7?years. Pandemic influenza-associated respiratory diseases included pneumonia (n?=?42), bronchitis (n?=?3), and pharyngitis (n?=?1). The median time from onset to admission was 3?days. Children were divided into those with severe (n?=?32) versus nonsevere illnesses (n?=?14) according to Japanese guidelines. Significant features in the severe group were younger age, previous asthmatic a...

2011-01-01

310

Abundance of West Nile virus mosquito vectors in relation to climate and landscape variables.

The antiviral action of common household disinfectants and antiseptics against murine hepatitis virus, a potential surrogate for SARS coronavirus

It is currently unclear if the potential for West Nile virus transmission by mosquito vectors in the eastern United States is related to landscape or climate factors or both. We compared abundance of vector species between urban and suburban neighborhoods of Henrico County, VA, in relation to the following factors: temperature, precipitation, canopy cover, building footprint, and proximity to drainage infrastructure. Mosquitoes were collected throughout the 2005, 2006, and 2007 seasons and tested for West Nile virus (WNV) in pools of 10-50. Test results of mosquito pools were compared to average site abundance from 37 sites in Henrico County, VA; abundance was then examined in relation to ecological variables. Urban infrastructure was positively correlated with the abundance of Culex pipiens L./Cx. restuans, and our findings implicate combined sewer overflow systems as large contributors to Culex vector populations. No measure of urbanization ...

2011-06-01

311

British Library Electronic Table of Contents (United Kingdom)

Background The 2003 outbreak of severe acute respiratory syndrome (SARS) infected over 8000 people and killed 774. Transmission of SARS occurred through direct and indirect contact and large droplet nuclei. The World Health Organization recommended the use of household disinfectants, which have not been previously tested against SARS coronavirus (SARS-CoV), to disinfect potentially contaminated environmental surfaces. There is a need for a surrogate test system given the limited availability of the SARS-CoV for testing and biosafety requirements necessary to safely handle it. In this study, the antiviral activity of standard household products was assayed against murine hepatitis virus (MHV), as a potential surrogate for SARS-CoV. Methods A surface test method, which involves drying an amo...

2009-01-01

312

Selective vulnerability of dorsal root ganglia neurons in experimental rabies after peripheral inoculation of CVS-11 in adult mice

British Library Electronic Table of Contents (United Kingdom)

The involvement of dorsal root ganglia was studied in an in vivo model of experimental rabies virus infection using the challenge virus standard (CVS-11) strain. Dorsal root ganglia neurons infected with CVS in vitro show prolonged survival and few morphological changes, and are commonly used to study the infection. It has been established that after peripheral inoculation of mice with CVS the brain and spinal cord show relatively few neurodegenerative changes, but detailed studies of pathological changes in dorsal root ganglia have not previously been performed in this in vivo experimental model. In this study, adult ICR mice were inoculated in the right hindlimb footpad with CVS. Spinal cords and dorsal root ganglia were evaluated at serial time points for histopathological and ultrastru...

2009-01-01

313

Rabies in the Face of the 21st Century

British Library Electronic Table of Contents (United Kingdom)

Summary Reference to an ancient Hindu picture of a snarling dog may be a convincing enough proof to consider the fact that rabies has been known in the world for the past 50 centuries. Prior to the monumental observation about rabies of Fracastoro in the 16th century, facts and fantasies were intermingled in the study of rabies. In the realm of fantasy, consider the statement of Aristotle (otherwise a great philosopher) that only animals and not humans die of rabies. It took 19 centuries before Fracastoro finally established that infection with rabies is lethal for all warm-blooded beings including humans. The new era of rabies dates from the time of Galtier who isolated the virus and Pasteur who was able to create a somewhat attenuated strain of virus fixe which became the tool of laborat...

2009-01-01

314

Quantitation of infectious myonecrosis virus in different tissues of naturally infected Pacific white shrimp, Litopenaeus vannamei, using real-time PCR with SYBR Green chemistry

British Library Electronic Table of Contents (United Kingdom)

The Pacific white shrimp, Litopenaeus vannamei, is the most important shrimp species in volume in world aquaculture. However, in recent decades, outbreaks of diseases, especially viral diseases, have led to significant economic losses, threatening the sustainability of shrimp farming worldwide. In 2004, Brazilian shrimp farming was seriously affected by a new disease caused by the Infectious myonecrosis virus (IMNV). Thus, disease control based on rapid and sensitive pathogen detection methods has become a priority. In this study, a specific quantitation method for IMNV was developed using real-time PCR with SYBR Green chemistry and viral load of the principal target tissues of chronically infected animals was quantified. The quantitative analysis revealed that mean viral load ranged from ...

2011-01-01

315

Molecular environment of the IIId subdomain of the IRES element of hepatitits C virus RNA on the human 40S ribosomal subunit

British Library Electronic Table of Contents (United Kingdom)

The molecular environment of the key subdomain IIId of the internal ribosome entry site (IRES) element of hepatitis C virus (HCV) RNA in the binary complex with the human 40S ribosomal subunit was studied. To this end, HCV IRES derivatives bearing perfluorophenylazido groups activatable by mild UV at nucleotides G263 or A275 in the subdomain IIId stem were used. They were prepared by the complementary addressed modification of the corresponding RNA transcript with alkylating oligodeoxyribonucleotide derivatives. None of the RNA derivatives were shown to be crosslinked to the 18S rRNA. It was found that the photoreactive groups of the IRES G263 and A275 nucleotides are crosslinked to ribosomal proteins S3a, S14, and S16. For the IRES derivative with the photoreactive group in nucleotide G26...

2009-01-01

316

Is XMRV a causal virus for prostate cancer?

British Library Electronic Table of Contents (United Kingdom)

The potential association between xenotropic murine leukaemia virus-related gammaretrovirus (XMRV) and prostate cancer (PCa) has been documented since 2006. It is important for furthering our understanding of the biological mechanisms of PCa to ascertain whether this association is causal. To summarize the available information on the epidemiological and laboratory findings of the association, we conducted a literature search of the PubMed electronic database (from March 2006 to February 2011) to identify relevant published studies that examined the association between XMRV and PCa. Although several studies showed the positive association between XMRV and PCa, more recent studies did not support this conclusion. The positive findings might be due to contamination of human samples. Further ...

2011-01-01

317

Inhibitory effect of ?/?-carrageenan from red alga Tichocarpus crinitus on the development of a potato virus X infection in leaves of Datura stramonium L.

British Library Electronic Table of Contents (United Kingdom)

The effect of ?/?-carrageenan from red alda Tichocarpus crinitus on the development of a potato virus X (PVX) infection in the leaves of Datura stramonium L. has been studied. The treatment of leaves with carrageenan stimulates a protein synthesis in the cells, causing an increase in the size of nucleoli and in the number of mitochondria and membranes of the rough endoplasmic reticulum. At the same time, such treatment slightly stimulates lytic processes, causing an increase in the number of smooth endoplasmic reticulum cisternae, dictyosomes, and cytoplasmic vacuoles and the formation of cytoplasmic electron-transparent zones. The carrageenan-induced stimulation of lytic processes results in the destruction of viral particles and can be considered as one of the defense mechanisms, prevent...

2010-01-01

318

Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with cervical carcinoma

Hepatitis C virus core upregulates the methylation status of the RASSF1A promoter through regulation of SMYD3 in hilar cholangiocarcinoma cells

Normal human foreskin keratinocytes cotransfected with the neomycin resistance gene and recombinant human papillomavirus (HPV) DNAs (types 16, 18, 31, and 33) that have a high or moderate association with cervical malignancy acquired immortality and contained integrated and transcriptionally active viral genomes. Only transcripts from the intact E6 and E7 genes were detected in at least one cell line, suggesting that one or both of these genes are responsible for immortalization. Recombinant HPV DNAs with low or no oncogenic potential for cervical cancer (HPV1a, -5, -6b, and -11) induced small G418-resistant colonies that senesced as did the nontransfected cells. These colonies contained only episomal virus DNA; therefore, integration of HPV sequences is important for immortalization of keratinocytes. This study suggests that the virus-encoded immortalization function contributes to the pathogenesis of cervical carcinoma.

1989-01-01

319

British Library Electronic Table of Contents (United Kingdom)

Increasing evidence has been accumulated indicating the important role of epigenetic regulation in tumor genesis. Previously, we observed that the transfection of hepatitis C virus core (HCVc) protein led to malignant transformation in normal biliary cells, and that tumor suppressor gene RASSF1A was downregulated in many hilar cholangiocarcinoma patients by hypermethylation in the promoter region. In the present study, we found SET and MYND domain-containing protein 3 (SMYD3), a novel histone methyltransferase, was overexpressed in cholangiocarcinoma patients especially in those with HCV infection. Transfection of HCVc into hilar cholangiocarcinoma cell lines QBC939 and FRH0201 could upregulate the expression of SMYD3 and promote cell growth, which was consistent with the results of our cl...

2011-01-01

320

Changing Treatment Paradigms Hepatitis C Virus in HIV-Infected Patients

British Library Electronic Table of Contents (United Kingdom)

The evaluation of hepatic histology and treatment of hepatitis C virus (HCV)/HIV-coinfected patients is rapidly changing. HCV has become an important cause of mortality in HIV-infected patients. Consequently, assessment of liver histology in all coinfected patients is particularly important. The evaluation of hepatic histology is shifting from reliance on the liver biopsy toward noninvasive modalities. Additionally, the importance of HCV-associated morbidity and mortality in HIV-infected patients has prompted increasing numbers of these patients to be HCV treatment candidates. Prospective trials in coinfected patients have reported lower sustained virologic responses compared to HCV-monoinfected patients. Consequently, the numbers of coinfected nonresponders to pegylated interferon/ribavir...

2007-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

321

Bortezomib induces apoptosis in T lymphoma cells and natural killer lymphoma cells independent of Epstein-Barr virus infection

British Library Electronic Table of Contents (United Kingdom)

Abstract Epstein-Barr virus (EBV), which infects not only B cells, but also T cells and natural killer (NK) cells, is associated with multiple lymphoid malignancies. Recently, the proteasome inhibitor bortezomib was reported to induce apoptosis of EBV-transformed B cells. We evaluated the killing effect of this proteasome inhibitor on EBV-associated T lymphoma cells and NK lymphoma cells. First, we found that bortezomib treatment decreased the viability of multiple T and NK cell lines. No significant difference was observed between EBV-positive and EBV-negative cell lines. The decreased viability in response to bortezomib treatment was abrogated by a pan-caspase inhibitor. The induction of apoptosis was confirmed by flow cytometric assessment of annexin V staining. Additionally, cleavage o...

2011-01-01

322

An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection

British Library Electronic Table of Contents (United Kingdom)

Oral vaccination is the most desirable immunization method by which to prevent fish disease occurring at the early larval stage. We developed an Escherichia coli-based oral nervous necrosis virus (NNV) subunit vaccine and demonstrated its effectiveness in orange spotted grouper (Epinephelus coioides) fry. However, the incubation period was determined to be over 2weeks. To enhance the efficacy and shorten the incubation period, a safe and digestible biological adjuvant should be found. Vibrio anguillarum, a common marine bacterium with immune-stimulatory capability was selected as an expression host. An expression vector containing its heat shock protein 60 gene (groE) as an inducible promoter was constructed to express recombinant NNV coat protein as an antigen. The recombinant V. anguilla...

2011-01-01

323

The law aspects of biotechnology patenting. The living source between discovery and invention; Aspetti giuridici del brevetto biotecnologico. La materia vivente tra scoperta e invenzione

Monte Carlo analysis of radiative transport in oceanographic lidar measurements

The aim of this work is to provide a comprehensive and consistent description of the complexity of laws regulating the field of biotechnology, mainly focusing on the definition of items to be patented, which is one of the most important and also controversial issues within the new 98/44/EC set of rules. As biotechnology is a specialist and multidisciplinary field, it has begun by giving a general description of the field, outlining the grounds for the new rules, and then thoroughly examined and evaluated previous national and international laws especially with regard to patenting. This was followed by an evaluation of what can be patented according to the new instructions by systematically assessing the section defining items to be patented in the field of biotechnology. It is used to be a matter of opinion whether biotechnological material was to be considered a discovery or an invention, the former being impatentable according to patenting laws. The new law pays particular attention ...

2001-07-01

324

AIDS and lung infection by Mycobacterium xenopi. Role of Computed Tomography; Sindrome da immunodeficienza acquisita e malattie polmonari da Mycobacterium xenopi. Ruolo della Tomografia Computerizzata

The analysis of oceanographic lidar systems measurements is often carried out with semi-empirical methods, since there is only a rough understanding of the effects of many environmental variables. The development of techniques for interpreting the accuracy of lidar measurements is needed to evaluate the effects of various environmental situations, as well as of different experimental geometric configurations and boundary conditions. A Monte Carlo simulation model represents a tool that is particularly well suited for answering these important questions. The PREMAR-2F Monte Carlo code has been developed taking into account the main molecular and non-molecular components of the marine environment. The laser radiation interaction processes of diffusion, re-emission, refraction and absorption are treated. In particular are considered: the Rayleigh elastic scattering, produced by atoms and molecules with small dimensions with respect to the laser emission wavelength (i.e. water molecules), ...

2001-07-01

325

Transformation of a car diesel engine with direct injection and common rail into a dual fuel engine; Trasformazione di un motore automobilistico diesel ad iniezione diretta dotato di common rail in un motore dual fuel

Mycobacterium xenopi is one of the most common agents responsible for nontubercolar mycobacterial pulmonary disease on AIDS patients. These lesions have been studied with conventional radiography while CT has been used in patients with aspecific mycobacterioses or non-AIDS pulmonary conditions from Mycobacterium xenopi. 12 AIDS patients were examined. They had pulmonary lesions from Mycobacterium xenopi, patients age ranged 30 to 46 years. All patients had CD4 blood levels lower than 250 cells/mL and Mycobacterium xenopi in the sputum. All patients underwent a standard chest radiograph and a CT examination. CT images were evaluated by three radiologists independently and the definitive diagnosis was made in the presence of a fourth radiologist. Chest CT showed parenchymal consolidation in 66% of cases, associated with bilateral basal bands in 16% of cases. Consolidation was unilateral in 41% of cases and most frequently involved the right lower lobe. Bilateral reticular interstitial ...

2000-06-01

326

The new industrial political tools based on the sustainable development of productive activities; Gli strumenti attuativi di una politica di sviluppo sostenibile dell'impresa

The reduced polluting emissions make natural gas a quite interesting alternative fuel for automotive applications. Therefore a car diesel engine has been transformed into a dual fuel engine with pilot injection via the common rail injection system used to ignite the methane-air charge. Standard injection pumps show a certain instability at low flow rates and high engine speed. On the opposite the new common rail system allows to ignite the fuel in all conditions with an amount of gas oil less than 8% of the entire energy required by the engine was enough to ignite the fuel. Furthermore, a power increase has been obtained, with an overall efficiency equal to or even higher than a conventional engine. The article deals with a series of test carried out on 1929 cm{sup 3} direct injection turbo-charged engine and presents the preliminary results. [Italian] La riduzione delle emissioni inquinanti rende il metano un combustibile alternativo piuttosto interessante per applicazioni ...

1999-08-01

327

Use of sodium aluminate in waste water treatment plants: wishes of saving money and innovating; Uso del aluminato sodico en las EDAR: una propuesta para innovar y economizar costes de explotacion

The new industrial political tools based on the sustainable development of productive activities, and originating at the Rio Conference in 1992, late introduced by the European Union, the Government and voluntary organizations (ISO, CEM, UNI) are gradually entering the market, thereby underling their effect in terms of industrial competitiveness. The main tools were examined in the report and mostly refer to the voluntary ones which, in surpassing the command and control logic, increasingly tend to get close to traditional fiscal, tariff and financial ones. Voluntary agreements, environmental management systems (EMAS, ISO14000), the risk prevention systems (UNI 10617), certification (Ecolabel, ISO 14040) and environment communications tools (environment balance sheet, environmental report, environmental statement) are synthetically described. Finally, the tools regarding the incentives still under Government concern an their diffusion are examined. [Italian] I nuovi strumenti di ...

1999-07-01

328

Unilateral NMR, 13C CPMAS NMR spectroscopy and micro-analytical techniques for studying the materials and state of conservation of an ancient Egyptian wooden sarcophagus

Most waste water treatment plants have processes to remove nutrients in order to avoid eutrophication in water receiving bodies. Regarding phosphorus removal. the most common option is chemical precipitation with ferric or aluminical precipitation with ferric or aluminium salts. We show here the successful experience carried out by the WWTP of Blanes and the company Safloc. A method ato remove phosphorus from waste water was developed by adding sodium aluminate. The use of this compound has turned out to be a sustainable way for this purpose in terms of costs, reliability and minimization of sludge production. (Author)

2006-07-01

329

British Library Electronic Table of Contents (United Kingdom)

A multi-technique approach was employed to study a decorated Egyptian wooden sarcophagus (XXV?XXVI dynasty, Third Intermediate Period), belonging to the Museo del Vicino Oriente of the Sapienza University of Rome. Portable non-invasive unilateral NMR was applied to evaluate the conservation state of the sarcophagus. Moreover, using unilateral NMR, a non-invasive analytical protocol was established to detect the presence of organic substances on the surface and/or embedded in the wooden matrix. This protocol allowed for an educated sampling campaign aimed at further investigating the state of degradation of the wood and the presence of organic substances by 13C cross polarization magic angle spinning (CPMAS) NMR spectroscopy. The composition of the painted layer was analysed by optical micr...

2011-01-01

330

The history of the coastal zones of the Great Caribbean tell through their sediments; La historia de las zonas costeras del Gran Caribe contada a traves de sus sedimentos

Synthesis by plasma and characterization of semiconductor compounds derived of polyacetylene; Sintesis por plasma y caracterizacion de compuestos semiconductores derivados del poliacetileno

The pollution of the aquatic zones grows with the time, principally due to industrial and urban settlements, among others, in the surroundings of these sites. The sources of this pollution can be identified by the study of the characteristics of the sediment, this gives relevant information to evaluate any aquatic system. Once the sediment is obtained, a series of preliminary tests are carry out (loss of ignition, 210{sup P}b dating) to be sure that we have a sedimentary core that hans .t be mixed, once this has be done we determine radionuclides, heavy metals and organic compounds. (Author) 2 refs.

2008-07-01

331

Softwood forest thinning as a biomass source for ethanol production: A feasibility study for California

In this work it is made a study of the structure and electric properties of chlorate polyethylene (PE-CI) with double and simple bonds obtained by continuous plasma with resistive coupling to 13.5 MHz. The synthesis conditions are power between 10 and 14 W and pressure of (6-7) x 10{sup -2} Torr. The synthesized PE-Cl in that way is soluble in acetone what indicates that probably is formed of short chains and not it shows the generalized inter crossing that is presented in some syntheses by plasma and that it can degrade the electric properties of these polymers. The IR and XPS analysis show the vibration of the C-C, C=C and C-CI bonds. The morphology of the polymer after being dissolved shows a compact and flat configuration. The electric conductivity has an approximately lineal behavior in an interval of 35 to 90% of relative humidity. (Author)

2003-07-01

332

Reconstructions of archaeological contexts in OpenGl environments; Intelligenza artificiale per i beni culturali e le biblioteche digitali. Atti del Workshop

A plan has been put forth to strategically thin northern California forests to reduce fire danger and improve forest health. The resulting biomass residue, instead of being open burned, can be converted into ethanol that can be used as a fuel oxygenate or an octane enhancer. Economic potential for a biomass-to-ethanol facility using this softwood biomass was evaluated for two cases: Stand-alone and co-located. The co-located case refers to a specific site with an existing biomass power facility owned by Wheelabrator Environmental Systems Inc. near Martell, California. A two-stage dilute acid hydrolysis process is used for the production of ethanol from softwoods, and the residual lignin is used to generate steam and electricity. For a plant processing 800 dry tonnes per day of feedstock, the co-located case is an economically attractive concept. Total estimated capital investment is approximately $ 70 million for the co-located case. The resulting internal rate of return is about 24% ...

2000-07-01

333

Project of the new measurement system for energy invoicing - Itaipu Hydroelectric Central; Proyecto del nuevo sistema de medicion de energia para facturacion de la Central Hidroelectrica de Itaipu

DVR project concerns the creation of a virtual reality OpenGL system for desktop applications in archaeology. The main goal is the creation of a 3D information system using the real time interaction of OpenGL environments. The first case study implemented is the Vettii House, one of the most important and outstanding monuments in Pompei. The virtual reconstruction of these architectural models is based on very detailed photogrammetric surveys and it is connected with information layers and archaeometric data-bases regarding the state of conservation of walls, structures and any type of materials of the monument. A full 3D interface characterizes the system, comprising a high resolution texturing with detailed volumetric and geometric data. Finally, for the future, the project plans to fit the system for scientific and end users through the installation of stations on site.

2001-07-01

334

Progressive neurostructural changes in adolescent and adult patients with bipolar disorder

Itaipu Binacional has taken a decision of executing a technological updating for the energy measurement system through the substitution of the current system by other one equipped with digital devices. The main reasons for that were the following: a) The equipment in operation were of an outdated generation b) The accuracy class of the existing equipment was of 0.5%, but the tendency for invoicing measurements was to install meters with accuracy class of 0.2% in the enterprises c) The requirements of ONS/MAE, in Brazil, which establishes the minimum requisites to be attended by invoicing measurement systems.

2001-07-01

335

British Library Electronic Table of Contents (United Kingdom)

Lisy ME, Jarvis KB, DelBello MP, Mills NP, Weber WA, Fleck D, Strakowski SM, Adler CM. Progressive neurostructural changes in adolescent and adult patients with bipolar disorder.-Bipolar Disord 2011: 13: 396-405. 2011 The Authors.-Journal compilation 2011 John Wiley & Sons A/S. Objectives:- Several lines of evidence suggest that bipolar disorder is associated with progressive changes in gray matter volume (GMV), particularly in brain structures involved in emotional regulation and expression. The majority of these studies however, have been cross-sectional in nature. In this study we compared baseline and follow-up scans in groups of bipolar disorder and healthy subjects. We hypothesized bipolar disorder subjects would demonstrate significant GMV changes over time. Methods:- A total of 58 ...

2011-01-01

336

Lecturas neobarrocas del Espejo de paciencia y reconceptualizaciones de la naci?n: Jos? Lezama Lima, Cintio Vitier y Severo Sarduy

British Library Electronic Table of Contents (United Kingdom)

This article examines the successive reinterpretations of one of Cuba?s foundational texts, namely the Espejo de paciencia (?Model of Patience??, 1608) by Silvestre de Balboa, as a baroque poem. The revalorization of the baroque by twentieth-century Cuban authors and critics has been explained as a consequence of the erasure of indigenous cultures and the subsequent imposition of a metropolitan culture. In this sense, the baroque is supposed to be paradigmatic for ?roots thinking?? about nation-building and culture. The readings of Balboa?s poem by neo-baroque writers such as Jos? Lezama Lima, Cintio Vitier and Severo Sarduy, however, put forward a transhistorical vision of the baroque and Cubanhood as an eternal and immutable phenomenon or, alternatively, a reading that privileges the tec...

2010-01-01

337

Kinetics of the methylparathion hydrolysis in aqueous medium; Cinetica de la hidrolisis del metilparation en medio acuoso

Evidence for a 4700-2100 BC palaeoearthquake recorded in a fluvial-archaeological sequence of the Segura River, SE Spain

The kinetics of alkaline hydrolysis of methylparathion was studied at different temperatures (0-50 Centigrade) in the p H range of 8-12 by ultraviolet-visible absorption spectroscopy. Optimum p H and wavelength conditions were defined to carry out the simultaneous determination of methylparathion and one of its hydrolysis product, paranitrophenol, in buffered aqueous medium. Based on the experimental data and the mathematical equation of the kinetics, a rate constant (k) of first-order and an activation energy (Ea) of 9.2 Kcal/mol, were estimated. (Author) activation energy (Ea) of 9.2 Kcal/mol, were estimated. (Author)

1997-12-31

338

British Library Electronic Table of Contents (United Kingdom)

The archaeological excavation of a rock shelter (Abrigo del Pozo) in one of the slopes of the Segura River (SE Spain) has revealed a exceptionally preserved sedimentary record spanning from the Paleolithic to the present-day, which includes an anomalous layer of stones (RFB) fallen from the roof. The sedimentary analysis of the stratigraphic sequence exhumed by the excavation indicates that human occupation of the rock shelter was controlled by fluvial environmental evolution. However, the RFB level resulted in a disturbance of human occupation and normal fluvial sedimentation. From the sedimentary and archaeological pieces of evidence, the RFB level has been interpreted as related to a palaeoearthquake responsible for the collapse of the roof and walls of the rock shelter. The palaeoearth...

2011-01-01

339

Enzymatic activity of the cellulolytic complex produced by Trichoderma reesei. Enzymatic hydrolysis of cellulose; Actividad enzimatica del complejo celulolitico producido por Trichoderma reesei. Hidrolisis enzimatica de la celulosa

Calcified metastases of the kidney. Etiology, pathogenesis, CT findings and diagnostic problems; Metastasi renali calcificate: eziologia, patogenesi, aspetti con tomografia computerizzata e diagnostica differenziale

The enzymatic activity characterization of the cellulolytic complex obtained from Trichoderma reesei QM 9414 and the influence of the enzymatic hydrolysis conditions on the hydrolysis yield are studied. Pure cellulose and native or alkali pretreated biomass Onopordum nervosum have been used as substrates. The values of pH, temperature, substrate concentration and enzyme-substrate ratio for the optimum activity of that complex, evaluated as glucose and reducing sugars production, have been selected. Previous studies on enzymatic hydrolysis of 0. nervosum have shown a remarkable effect of the alkaline pretreatments on the final hydrolysis yield. (Author) 10 refs.

1986-07-01

340

Translation products of pre-S(1), pre-S(2) regions and the S gene of hepatitis B virus: susceptibility of their antigenic activities to treatment with heat, urea formalin or pepsin

Aim of the work was to describe computerised tomography (CT) findings of calcified renal metastases focusing on differential diagnosis problems. Abdominal CT scans of 8000 patients with tumor, performed from 1984 to 1998, have been retrospectively reviewed. [Italian] Scopo del lavoro e' quello di descrivere gli aspetti con tomografia computerizzata delle metastasi renali calcificate enfatizzando le problematiche diagnostico differenziali. Sono state valutate retrospettivamente le indagini TC addominali di 8000 pazienti affetti da tumore nel periodo 1984-1998, analizzando le lesioni calcificate riscontrate.

2000-03-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

341

Therapy-induced selective loss of leukemia-initiating activity in murine adult T cell leukemia

Hepatitis B subviral particles, purified from plasma of asymptomatic carriers seropositive for hepatitis B e antigen, were treated with various conditions reported for the processing of vaccines. Thereafter,...Full Text Available

1986-12-01

342

The mitogenic activity of human T-cell leukemia virus type I is T-cell associated and requires the CD2/LFA-3 activation pathway.

Chronic HTLV-I (human T cell lymphotropic virus type I) infection may cause adult T cell leukemia/lymphoma (ATL), a disease with dismal long-term prognosis. The HTLV-I transactivator, Tax, initiates...Full Text Available

2010-12-20

343

Roles of the TNF receptor-associated factor (TRAF)2/3 binding site in differential B cell signaling by CD40 and its viral oncogenic mimic, latent membrane protein 1 (LMP1)

The presence of a high number of activated T cells in the bloodstream and spontaneous proliferation of peripheral blood mononuclear cells in vitro are striking characteristics of human T-cell leukemia...Full Text Available

1993-06-01

344

Replication, Pathogenesis and Transmission of Pandemic (H1N1) 2009 Virus in Non-Immune Pigs

The Epstein-Barr virus protein, LMP1, is a functional mimic of the cellular receptor CD40, but signals to B lymphocytes in an amplified and sustained manner compared to CD40. LMP1 contributes...Full Text Available

2009-09-01

345

Patient- and delivery-level factors related to acceptance of HIV counseling and testing services among tuberculosis patients in South Africa: a qualitative study with community health workers and program managers

The declaration of the human influenza A pandemic (H1N1) 2009 (H1N1/09) raised important questions, including origin and host range [1],...Full Text Available

346

No difference in antibody titers against xenotropic MLV related virus in prostate cancer cases and cancer-free controls

BackgroundSouth Africa has a high tuberculosis (TB)-human immunodeficiency virus (HIV) coinfection rate of 73%, yet only 46% of TB patients are tested for HIV. To date, relatively...Full Text Available

347

British Library Electronic Table of Contents (United Kingdom)

New ELISA assays were developed to measure immunoreactivity for XMRV. Antibody titers were measured in a cohort of prostate cancer cases and cancer free controls from the central United States. No statistically significant differences were observed in immunoreactivity between cases and controls for either the XMRV-env or the XMRV-gag antigen.

2011-01-01

348

Model system using coliphage phi X174 for testing virus removal by air filters.

Mobilization of the genetically engineered plasmid pHSV106 from Escherichia coli HB101(pHSV106) to Enterobacter cloacae in drinking water.

Short-term (15-min-duration) and long-term (5- to 6-day-duration) test procedures have been developed for determining the efficiency of the removal of bacteriophage phi X174 by air-sterilizing filters....Full Text Available

1992-03-01

349

Lytic HSV-1 infection induces the multifunctional transcription factor Early Growth Response-1 (EGR-1) in rabbit corneal cells

We have used triparental matings to demonstrate transfer (mobilization) of the nonconjugative genetically engineered plasmid pHSV106, which contains the thymidine kinase gene of herpes simplex virus...Full Text Available

1991-01-01

350

LY294002 may overcome 5-FU resistance via down-regulation of activated p-AKT in Epstein-Barr virus-positive gastric cancer cells

BackgroundHerpes simplex virus type-1 (HSV-1) infections can cause a number of diseases ranging from simple cold sores to dangerous keratitis and lethal encephalitis. The interaction...Full Text Available

351

Infection disclosure in the injecting dyads of Hungarian and Lithuanian injecting drug users who self-reported being infected with hepatitis C virus or human immunodeficiency virus.

BackgroundAs EBV-associated gastric cancer has unique features that are different from EBV (-) gastric cancer, EBV is considered to have a key role in gastric carcinogenesis. It...Full Text Available

352

Hepatitis B virus-induced defect of monocyte-derived dendritic cells leads to impaired T helper type 1 response in vitro: mechanisms for viral immune escape

The aim of this study was to assess the prevalence and correlates of disclosure to network members of being hepatitis C virus (HCV)- or human immunodeficiency virus (HIV)-infected among injecting dyads of infected injection drug users (IDUs) in Budapest, Hungary and Vilnius, Lithuania,. Multivariate generalized estimating equations (GEE) were used to assess associations. Very strong infection disclosure norms exist in Hungary, and HCV disclosure was associated with using drugs and having sex within the dyad. Non-ethnic Russian IDUs in Lithuania were more likely to disclose HCV infection to non-Roma, emotionally close and HCV-infected network members, and to those with whom they shared cookers, filters, drug solutions or rinse water or got used syringes from, and if they had fewer non-IDU or IDU network members. Ethnic Russian Lithuanian IDUs were more likely to disclose HCV if they had higher disclosure attitude and knowledge scores, 'trusted' ...

2010-09-15

353

Ewald sphere correction for single-particle electron microscopy

Dendritic cells (DC) are the most potent antigen-presenting cells and play a central role in the induction of antiviral immune responses. Recently, we have shown that monocyte-derived DC (MoDC) from...Full Text Available

2003-08-01

354

Evolution of Hepatitis B Virus in a Chronic HBV-Infected Patient over 2 Years

Most algorithms for three-dimensional (3D) reconstruction from electron micrographs assume that images correspond to projections of the 3D structure. This approximation limits the attainable resolution of the reconstruction when the dimensions of the structure exceed the depth of field of the microscope. We have developed two methods to calculate a reconstruction that corrects for the depth of field. Either method applied to synthetic data representing a large virus yields a higher resolution reconstruction than a method lacking this correction.

2006-03-15

355

Efficacy of the Carbocyclic 2?-Deoxyguanosine Nucleoside BMS-200475 in the Woodchuck Model of Hepatitis B Virus Infection

Mutations in full-length HBV isolates obtained from a chronic HBV-infected patient were evaluated at three time points: 1 day, 6 months, and 31 months. While 5 nucleotides variation, and an 18 bp...Full Text Available

2011-01-01

356

Efficacy of a heat inactivated hepatitis B vaccine in male homosexuals: outcome of a placebo controlled double blind trial.

Daily oral treatment with the cyclopentyl 2′-deoxyguanosine nucleoside BMS-200475 at doses ranging from 0.02 to 0.5 mg/kg of body weight for 1 to 3 months effectively reduced the level of woodchuck...Full Text Available

1998-12-01

357

Dissecting toxin immunity in virus-infected killer yeast uncovers an intrinsic strategy of self-protection

The efficacy of a heat inactivated hepatitis B virus vaccine, containing 3 micrograms hepatitis B surface antigen (HBsAg), was studied in a high risk group of 800 susceptible homosexual men by a randomised...Full Text Available

1983-04-23

358

Decay-accelerating factor CD55 is identified as the receptor for echovirus 7 using CELICS, a rapid immuno-focal cloning method.

Toxin-secreting “killer” yeasts were initially identified >40 years ago in Saccharomyces cerevisiae strains infected with a double-stranded RNA “killer”...Full Text Available

2006-03-07

359

Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 Nucleic Acid Extraction Systems for Detection of RNA and DNA Respiratory Viruses in Nasopharyngeal Aspirate Samples?

Using an anti-receptor mAb that blocks the attachment of echovirus 7 and related viruses (echoviruses 13, 21, 29 and 33), we have isolated a complementary DNA clone that encodes the human decay-accelerating...Full Text Available

1994-11-01

360

Cellular DNA region involved in induction of thymic lymphomas (Mlvi-2) maps to mouse chromosome 15.

The NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid...Full Text Available

2008-07-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

361

CNS Expression of B7-H1 Regulates Pro-Inflammatory Cytokine Production and Alters Severity of Theiler's Virus-Induced Demyelinating Disease

Two cellular DNA regions representing common domains for proviral DNA integration ( Mlvi -1 and Mlvi -2) have been identified in Moloney murine leukemia virus-induced rat thymic lymphomas. Cellular...Full Text Available

1984-05-01

362

Amino-Terminal Region of the Human Immunodeficiency Virus Type 1 Nucleocapsid Is Required for Human APOBEC3G Packaging

The CNS is a unique organ due to its limited capacity for immune surveillance. As macrophages of the CNS, microglia represent a population originally known for the ability to assist neuronal stability,...Full Text Available

363

Subsidence monitoring in the Cerro Prieto geothermal field, B.C., Mexico; Monitoreo de la subsidencia en el campo geotermico de Cerro Prieto, B.C., Mexico

APOBEC3G exerts its antiviral activity by targeting to retroviral particles and inducing viral DNA hypermutations in the absence of Vif. However, the mechanism by which APOBEC3G is packaged into virions...Full Text Available

2004-11-01

364

Simulations of the design basis accident at conditions of power increase and the o transient of MSIV at overpressure conditions of the Laguna Verde Power Station; Simulaciones del accidente base de diseno a condiciones de aumento de potencia y del transitorio de cierre de MSIV a condiciones de sobrepresion de la Central Laguna Verde

Since 1977, precise leveling surveys have been carried out to determine whether there have been vertical terrain displacements and the subsidence speed rate in the Cerro Prieto Geothermal field area. The elevation contours for each leveling show since 1962 that there was a basin that involves the geothermal field. The differences in the last three levelings show a generalized subsidence between Cerro Prieto (BN 10049) and Imperial faults (BN CD-14). [Spanish] A partir de 1977 se realizan trabajos de nivelacion de precision, con el fin de conocer si existen desplazamientos verticales del terreno y cual es la velocidad de subsidencia que existe en el area del Campo Geotermico de Cerro Prieto. Las configuraciones de cotas para cada uno de los anos nivelados, muestran que desde 1962 existe una cuenca que incluye al campo geotermico; la diferencia de cotas obtenidas en las tres ultimas nivelaciones, indican una subsidencia generalizada entre la ...

1999-04-01

365

Politics and instruments for a sustainable development; Politiche e strumenti per uno sviluppo sostenibile

This document presents the analysis of the simulation of the loss of coolant accident at uprate power conditions, that is 2027 MWt (105% of the current rated power of 1931MWt). This power was reached allowing an increase in the turbine steam flow rate without changing the steam dome pressure value at its rated conditions (1020 psiaJ. There are also presented the results of the simulation of the main steam isolation va/ve transient at overpressure conditions 1065 psia and 1067 MWt), for Laguna Verde Nuclear Power Station. Both simulations were performed with the best estimate computer code TRA C BF1. The results obtained in the loss of coolant accident show that the emergency core coolant systems can recover the water level in the core before fuel temperature increases excessively, and that the peak pressure reached in the drywell is always below its design pressure. Therefore it is concluded that the integrity of the containment is not challenged during a loss of coolant accident at ...

2001-07-01

366

Photovoltaic plants in the electronic system; Gli impianti fotovolatici nel sistema elettrico. Stato e prospettive

The new industrial political tools based on the sustainable development of productive activities, and originating at the Rio Conference in 1992, late introduced by the EU, the Government and voluntary organizations (ISO, CEM, UNI) are gradually entering the market, thereby underling their effect in terms of industrial competitiveness. The main tools were examined in the report and mostly refer to the voluntary ones which, in surpassing the 'command and control' logic, increasingly tend to get close to traditional fiscal, tariff and financial ones. Voluntary agreements, environmental management systems (EMAS, ISO 14001), the risk prevention systems (UNI 10617), certification (Eco label, ISO 14040) and environment communication tools (environment balance sheet, environmental report, environmental statement) are synthetically described. [Italian] I nuovi strumenti di politica industriale orientati allo sviluppo sostenibile delle attivita' produttive che traggono ...

1999-06-01

367

Fracture Characteristics in a Disposal Pit on Mesita del Buey, Los Alamos National Laboratory

The article provides a 1998 updated picture of Italy's and the world's photovoltaic market in terms of produced modules and total installed capacity, as well as market growth forecasts up to 2010. After a short description of the state-of-the-art of cell and module manufacturing, ana analysis of the cost of producing a photovoltaic kw is reported for different plant types: stand-alone plants with energy storage batteries, plants connected to low low voltage networks or intended for supporting medium voltage networks, hybrid plants with diesel sets. The article is concluded by illustrating ENEL's (Electric Power Production Company) engagement in the field of photovoltaic solar energy as regards theoretical studies, research and testing of new technologies, and installing plants; over nearly twenty years of activity, ENEL has designed and built a few hundreds of photovoltaic plants for a total capacity of about 4.000 kw, and is currently in the process of ...

1999-04-01

368

Experimental evaluation of the production of the poisons Xe-135 and Sm-149 of the TRIGA Mark III reactor with mixed core, configuration No. 16 (Final report of the project); Evaluacion experimental de la produccion de los venenos Xe-135 y Sm-149 del reactor TRIGA Mark III con nucleo mixto, config. No. 16 (Informe final del proyecto)

The characteristics of fractures in unit 2 of the Tshirege Member of the Bandelier Tuff were documented in Pit 39, a newly excavated 13.7 m deep disposal pit at Material Disposal Area G on Mesita del Buey. The average spacing between fractures is about 1.0 to 1.3 m, the average fracture aperture is about 3 to 5 mm, and the average fracture dip is about 76o to 77o. Fracture spacing and dip in Pit 39 are generally consistent with that reported from other fracture studies on the Pajarito Plateau, although the fracture apertures in Pit 39 are less than reported elsewhere. Measured fracture orientations are strongly affected by biases imparted by the orientations of the pit walls, which, combined with a small data set, make identification of potential preferred orientations dlfflcult. The most prominent fracture orientations observed in Pit 39, about E-W and N20E, are often not well represented elsewhere on the Pajarito Plateau. Fracture fills contain smectite to about ...

1998-12-01

369

Eco-compatible products and processes development. ENEA promotion actions; Sviluppo di prodotti e processi ecocompatibili. Le attivita' dell'ENEA

It was generated the concentration curve of the Xe{sup 135} (t) during the TRIGA Mark III reactor operation cycle, for a continuous irradiation of 72 h to 1 MW of thermal power, as well as the accumulation curve of the isotope after the shutdown, for the fuel configuration No. 16 in the thermal column. The maximum negative reactivities generated by the Xe{sup 135} for operation times greater than 60 h to 1 MW and after the reactor shutdown its were of 1.968 {+-} 0.15 dollars and 2.30 {+-} 0.15 dollars respectively. When comparing these results with those theoretically calculated we find differences of the order of 3.6% and 5.34% which are understood inside the experimental error that on the average was of 7.6%. The results before mentioned have an important application during the start up process of the Reactor, when analyzing the value of the weekly reactivity excess of the core and when is choice the pattern of bars to use for experiments of but of 2 h, where is required to minimize ...

1991-11-15

370

Helical CT defecography; La defecografia con Tomografia Computerizzata spirale

The new industrial political tools based on the Sustainable development of productive activities and originating at the Rio Conference in 1992, late introduced by the EU, the Government and voluntary organizations (ISO, CEM, UNI) are gradually entering the market, thereby underlying their effect in terms of industrial competitiveness. The main tools were examined in this report and mostly refer to the voluntary ones which, in surpassing the Command and Control logic, increasingly tend to get close to traditional fiscal, tariff and financial ones. [Italian] I nuovi strumenti di politica industriale orientali allo Sviluppo Sostenibile delle attivita' produttive che traggono origine dalla Conferenza di Rio del 1992 e sono stati introdotti successivamente da pare della Comunita' Europea, dal Governo e dagli Organismi di normazione volontaria (ISO, CEN, UNI), stanno gradualmente penetrando sul mercato facendo valere i loro effetti in termini di ...

2001-07-01

371

Gastric stromal tumors. CT findings; Tumori stromali gastrici. Aspetti con Tomografia Computerizzata

The purpose of this work is to investigate the possible role of Helical CT defecography in pelvic floor disorders by comparing the results of the investigations with those of conventional defecography. The series analyzed consisted of 90 patients, namely 62 women and 28 men, ranging in age 24-82 years. They were all submitted to conventional defecography, and 18 questionable cases were also studied with Helical CT defecography. The conventional examination was performed during the 4 standard phases of resting, squeezing, Valsalva and straining; it is used a remote-control unit. The parameters for Helical CT defecography were: 5 mm beam collimation, pitch 2, 120 KV, 250 m As and 18-20 degrees gantry inclination to acquire coronal images of the pelvic floor. The rectal ampulla was distended with a bolus of 300 mL nonionic iodinated contrast agent (dilution: 3g/cc). The patient wore a napkin and was seated on the table, except for those who could not hold the position and were thus ...

1999-11-01

372

Multiphasic helical Computed Tomography of hepatocellular carcinoma. Evaluation after various percutaneous ablation procedures; Tomografia Computerizzata spirale multifasica dell'epatocarcinoma. Valutazione dopo differenti procedure ablative percutanee

Gastric stromal tumors are an ill-defined group of lesions arising from muscle wall cells and characterized by extremely variable biological patterns. Thanks to modern immunohistochemical and ultrastructural techniques, four main classes of these lesions have been identified, namely: (1) tumors with differentiation toward smooth muscle cells; (2) tumors with differentiation toward neural elements; (3) tumors with differentiation toward neural elements; (3) tumors with dual differentiation toward either cell type. It was investigated the yield of CT in diagnosing and characterizing gastric stromal tumors. It was retrospectively reviewed the CT findings of 38 patients (15 men and 23 women; mean age 51 years) with pathologically proven gastric stromal tumors, namely 31 of myoid origin, 4 of neural origin, 2 with both muscle and neural differentiation, 1 lacking differentiation with either cell type. The myoid tumors involved gastric fundus in 9/13 and 5/7 benign lesions and lesions with ...

2000-02-01

373

Helical CT of traumatic injuries of the thoracic aorta; Applicazioni della Tomografia Computerizzata spirale nello studio delle lesioni traumatiche dell'aorta toracica

The purpose of this paper is to report the personal experience with helical CT evaluation of hepatocellular carcinoma treated with various percutaneous interventional procedures. From December 1996 to September 1998 it were examined with helical CT 41 patients (73 nodules in all) with hepatocellular carcinoma treated with percutaneous ablation therapies: conventional ethanol injection in 18 subjects (31 nodules), one-shot ethanol injection 3 (8 nodules), radiofrequency thermal ablation in 16 (25 nodules), and combined chemo embolization and ethanol injection in 4 (9 nodules). CT performed was 4-27 days after the last session, acquiring biphasic volumetric images in 14 patients and triphasic volumetric images in 27. A second treatment with subsequent CT study was performed for 28 lesions; 15 underwent 3 serial studies and 6 underwent 4 studies. Compared with pretreatment findings, the diameter was unchanged in 62% of the nodules and increased in 38%. Morphology was unchanged in 63% of ...

1999-12-01

374

Multistep process of neoplastic transformation of normal human fibroblasts by 60Co gamma rays and Harvey sarcoma viruses

Acute thoracic aortic injuries account for up to 10-20% of fatalities in high-speed deceleration road accidents and have an estimated immediate fatality rate of 80-90%. Untreated survivors to acute trauma (10-20%) have a dismal prognosis: 30% of them die within 6 hours, 40-50% die within 24 hours, and 90% within 4 months. It was investigated the diagnostic accuracy of Helical Computed Tomography (Helical CT) in acute traumatic injuries of the thoracic aorta, and the role of this technique in the diagnostic management of trauma patients with a strong suspicion of aortic rupture. It was compared retrospectively the chest Helical CT findings of 256 trauma patients examined June 1995 through August 1999. Chest Helical CT examinations were performed according to trauma score, to associated traumatic lesions and to plain chest radiographic findings. All the examinations were performed with no intravenous contrast agent administration and the pitch 2 technique. After a previous baseline ...

2000-09-01

375

Magnetic resonance imaging: early detection of central nervous system involvement in acquired immunodeficiency syndrome (AIDS)

As reported previously (Namba et al., 1985), normal human fibroblasts were transformed by 60Co gamma-ray irradiation into immortal cells with abnormal karyotypes. These transformed cells (KMST-6), however, showed a low cloning efficiency in soft agar and no transplantability. However, upon treatment with Harvey murine sarcoma virus (Ha-MSV), the cells acquired elevated clonability in soft agar and transplantability in nude mice. Ha-MSV alone, however, did not convert normal human fibroblasts into either immortal or tumorigenic cells. The Ha-MSV-transformed KMST-6 cells showed an enhanced expression of the ras oncogene, but normal and 60Co gamma-ray-transformed cells did not. Our current data suggest that gamma rays worked against normal human cells as an initiator, giving rise to chromosome aberrations and immortality, and that Ha-MSV, probably through its ras oncogene, played a role in the progression of the malignant cell population to a more malignant one ...

1986-03-15

376

Hepatic aflatoxin B1-DNA adducts and TP53 mutations in patients with hepatocellular carcinoma despite low exposure to aflatoxin B1 in southern Japan

Central Nervous System (CNS) involvement, whether primary by the Human Immunodeficiency Virus - HIV - itself, or secondary (toxoplasmosis or lymphoma) is remarkably frequent in AIDS, in 40 to 70% of cases, depending upon the author. In order to study the natural history of this illness, a cohort of 25 asymptomatic seropositive patients have been established. Every 6 months these patients undergo biological and clinical examinations, as well as Magnetic Resonance brain scans. After two examinations at a 6 month's interval, the first results are reported. Out of these 25 cases, 9 present anomalies: One patient with diffuse cerebral atrophy and 8 others with high signal intensity areas on T2 weighted sequences, like those of the Multiple Sclerosis. No relationship could be demonstrated between the existence of these lesions and various criteria such as age, sex, risk factors and T4 cells count. The nature of these lesions is not lear. They certainly indicate early ...

377

British Library Electronic Table of Contents (United Kingdom)

Abstract Background & aims: Hepatitis B or C virus infection is considered to be the main cause of hepatocellular carcinoma (HCC) in Japan. Aflatoxin B1 (AFB1) is a carcinogen associated with HCC in regions with high exposure. Mutations in codon 249, exon 7 are a hallmark of AFB1 exposure. Therefore, to clarify the role of AFB1 in hepatocarcinogenesis, we examined AFB1-DNA in liver tissue and sequenced TP53 in Japanese patients with HCC. Methods: Hepatocyte AFB1-DNA adducts were determined immunohistochemically and direct sequencing of TP53 was done to determine mutations in 188 of 279 patients who underwent hepatic resection for HCC. We assessed hepatitis C virus antibodies (HCV Ab) and HBSAg expression; patients without either were defined as having non-B non-C hepatocellular carcinoma (...

2011-01-01

378

GROWTH REGULATION IN ROUS SARCOMA VIRUS INFECTED CHICKEN EMBRYO FIBROBLASTS: THE ROLE OF THE src GENE

Functional expression of anti-hepatitis B virus (HBV) preS2 antigen scFv by cspA promoter system in Escherichia coli and application as a recognition molecule for single-walled carbon nanotube (SWNT) field effect transistor (FET)

We report here a study of the mechanisms leading to loss of growth control in chicken embryo fibroblasts transformed by Rous sarcoma virus (RSV). We have been particularly concerned with the role of the src gene in this process, and have used RSV mutants temperature sensitive (ts) for transformation to investigate the nature of the growth regulatory lesion. The two principal findings were (1) the stationary phase of the cell cycle (G{sub 1}) in chick embryo fibroblasts seems to have two distinct regulatory compartments (using the terminology of Brooks et al. we refer to these as 'Q' and 'A' states). When rendered stationary at 41.5 C by serum deprivation, normal cells enter a Q state, but cells infected with the ts-mutant occupy an A state. (2) Whereas normal cells can occupy either state depending on culture conditions, the ts-infected cells, at 41.5 C, do not seem to enter Q even though a known src gene product, a kinase, is ...

1980-07-01

379

British Library Electronic Table of Contents (United Kingdom)

The preS2 antigens of hepatitis B virus (HBV), which causes a serious health problem in the world, have been implicated in hepatocyte cell binding and viral penetration. Therefore, the importance of antibody production against preS2 antigen for early diagnosis of HBV has been well established. In this study, the recombinant HBV preS2 single chain variable fragment (scFv) antibody was successfully expressed in E. coli with the novel cold shock vector (pCold) under the cspA promoter, and its expression level was compared with the pET vector under the T7 promoter. Additionally, a host with an oxidizing cytoplasm, E. coli trxB/gor double mutant, was used to improve the soluble expression. The anti-HBV preS2 scFv using pCold vector was successfully expressed in a soluble and functional form in ...

2010-01-01

380

Evaluation of the effectiveness of an antimicrobial air filter to avoid porcine reproductive and respiratory syndrome virus (PRRSV) aerosol transmission, after 16 months of exposure to a commercial swine environmental conditions

Development of recombinant adeno-associated virus and adenovirus cocktail system for efficient hTERTC27 polypeptide-mediated cancer gene therapy

The effectiveness of Noveko's antimicrobial filter was evaluated after 16 months of exposure to commercial swine production. The experiment involved the use of a scaled model of a commercial swine facility consisting of 2 small chambers connected by a duct containing the filters. A 5 kg naive pig was placed in the reception chamber for a period of 6 hours after aerosolization with porcine reproductive and respiratory syndrome virus (PRRSV). Blood samples from pigs were collected before and after aerosolization to test for the presence of PRRSV RNA. Only blood samples were tested for PRRSV antibodies by IDEXX 2XR ELISA. None of the 9 pigs tested were found to be infected. The study showed that the technology used to integrate the antimicrobial agent into the filter fibers allows the filter combination to withstand extreme weather and endure commercial swine production for at least 16 months, and can maintain its effectiveness to avoid airborne transmission ...

2010-07-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

381

British Library Electronic Table of Contents (United Kingdom)

The low in vivo transduction efficiency of recombinant adeno-associated virus (rAAV) and the undesirably strong immunogenicity of adenovirus (rAdv) have limited their clinical utilization in cancer gene therapy. We have previously demonstrated that intratumoral injection of rAAV expressing a C-terminal polypeptide of human telomerase reverse transcriptase (rAAV-hTERTC27) effectively inhibits the growth of glioblastoma xenografts in nude mice. To further improve its efficacy, we combined rAAV-hTERTC27 with rAdv and investigated the efficiency of the cocktail vectors in vivo. At a nontherapeutic dose (1 x 108 plaque-forming units (PFUs)), rAdv-null and rAdv-hTERTC27 were equipotent in enhancing the therapeutic efficacy of rAAV-hTERTC27 (1.5 x 1011?v.g.), and complete tumor regression w...

2008-01-01

382

Development of a multiplex PCR method for simultaneous detection of diagnostic DNA markers of five disease and pest resistance genes in potato

British Library Electronic Table of Contents (United Kingdom)

Multiplex PCR is practically a reasonable choice for molecular marker-assisted selection in potato breeding. We had developed and were using a multiplex PCR method for selection of resistance genes to cyst nematode (H1), Potato virus X (Rx1) and late blight (R1 and R2). Since then, more reliable and tightly linked markers for H1 and R2, and a new marker for resistance to Potato virus Y (Ry chc ) were developed. In this article, all these superior markers, including a positive marker to eliminate PCR-failed samples, were incorporated into one multiplex PCR assay. Using the newly developed multiplex PCR technique, five plants potentially harboring all five resistance genes were selected from 96 hybrid plants approximately 5?h after DNA extraction, which is a third of the operation time compa...

2011-01-01

383

State of Surgery in Tropical Africa: A Review

British Library Electronic Table of Contents (United Kingdom)

This is a review of recently published literature on surgery in tropical Africa. It presents the current state of surgical need and surgical practice on the continent. We discuss the enormous burden of surgical pathology (as far as it is known) and the access to and acceptability of surgery. We also describe the available facilities in terms of equipment and manpower. The study looked at the effects of the human immunodeficiency virus, the role of traditional healers, anesthesia, and the economics of surgery. Medical training and research are discussed, as are medical migration out of Africa and the concept of task shifting, where surgical procedures are performed by others when surgeons are not available. It closes with recommendations for involvement and action in this area of great glob...

2011-01-01

384

Clinical aspects of Marburg hemorrhagic fever

British Library Electronic Table of Contents (United Kingdom)

Marburg virus belongs to the genus Marburgvirus in the family Filoviridae and causes a severe hemorrhagic fever, known as Marburg hemorrhagic fever (MHF), in both humans and nonhuman primates. Similar to the more widely known Ebola hemorrhagic fever, MHF is characterized by systemic viral replication, immunosuppression and abnormal inflammatory responses. These pathological features of the disease contribute to a number of systemic dysfunctions including hemorrhages, edema, coagulation abnormalities and, ultimately, multiorgan failure and shock, often resulting in death. A detailed understanding of the pathological processes that lead to this devastating disease remains elusive, a fact that contributes to the lack of licensed vaccines or effective therapeutics. This article will review the...

2011-01-01

385

Advances in molecular biology: impact on rotavirus vaccine development.

Virostatic potential of micro-nano filopodia-like ZnO structures against herpes simplex virus-1.

The first candidate rotavirus vaccine was a live attenuated oral vaccine made by the classical empirical method of serial passage of virus in tissue culture cells. Current tetravalent vaccine candidates that are in the final stages of efficacy testing in the United States were made by genetic reassortment. This article briefly highlights how advances in the basic understanding of the molecular biology of rotaviruses have facilitated vaccine development. New approaches for second-generation vaccines and improvements in vaccine efficacy based on further exploitation of the tools and knowledge of rotavirus molecular biology and pathogenesis are discussed. PMID:8752289

1996-09-01

386

Viral RNA testing and automation on the bead-based CBNE detection microsystem.

Herpes simplex virus type-1 (HSV-1) entry into target cell is initiated by the ionic interactions between positively charged viral envelop glycoproteins and a negatively charged cell surface heparan sulfate (HS). This first step involves the induction of HS-rich filopodia-like structures on the cell surface that facilitate viral transport during cell entry. Targeting this initial first step in HSV-1 pathogenesis, we generated different zinc oxide (ZnO) micro-nano structures (MNSs) that were capped with multiple nanoscopic spikes mimicking cell induced filopodia. These MNSs were predicted to target the virus to compete for its binding to cellular HS through their partially negatively charged oxygen vacancies on their nanoscopic spikes, to affect viral entry and subsequent spread. Our results demonstrate that the partially negatively charged ZnO-MNSs efficiently trap the virions via a novel virostatic mechanism rendering them unable to enter into ...

2011-08-26

387

Comparative analysis of budded virus infectivity of Bombyx mandarina and B. mori nucleopolyhedroviruses.

We developed prototype chemistry for nucleic acid hybridization on our bead-based diagnostics platform and we established an automatable bead handling protocol capable of 50 part-per-billion (ppb) sensitivity. We are working towards a platform capable of parallel, rapid (10 minute), raw sample testing for orthogonal (in this case nucleic acid and immunoassays) identification of biological (and other) threats in a single sensor microsystem. In this LDRD we developed the nucleic acid chemistry required for nucleic acid hybridization. Our goal is to place a non-cell associated RNA virus (Bovine Viral Diarrhea, BVD) on the beads for raw sample testing. This key pre-requisite to showing orthogonality (nucleic acid measurements can be performed in parallel with immunoassay measurements). Orthogonal detection dramatically reduces false positives. We chose BVD because our collaborators (UC-Davis) can supply samples from persistently infected animals; and because ...

2008-09-01

388

A single amino acid substitution modulates low-pH-triggered membrane fusion of GP64 protein in Autographa californica and Bombyx mori nucleopolyhedroviruses

Bombyx mandarina nucleopolyhedrovirus (BomaNPV) is a variant of Bombyx mori nucleopolyhedrovirus (BmNPV). BomaNPV S1 strain has been reported to be significantly less virulent than the BmNPV T3 strain via the oral infection route in B. mori larvae, but other features of S1 including budded virus (BV) infectivity and virus propagation in cultured cells are still unknown. In this study, we compared BV infectivity of S1 and T3 in B. mori larvae and cultured cells. Larval bioassays by intrahemocoelic BV injection revealed that the median lethal dose of S1's BV was approximately three times lower than that of T3. In addition, S1 produced more BVs and occlusion bodies (OBs) in the hemolymph of B. mori larvae compared with T3. Furthermore, we observed that the locomotion was enhanced earlier and the median lethal time was shorter in S1-infected larvae compared with those in T3-infected larvae. Western blot analysis of S1- and T3-infected BmN cells ...

2011-05-22

389

Voltage and reactive power control by means of static compensators of vars in electric power systems; Control de voltaje y potencia reactiva mediante compensadores estaticos de Vars en sistemas electricos de potencia

We have previously shown that budded viruses of Bombyx mori nucleopolyhedrovirus (BmNPV) enter the cell cytoplasm but do not migrate into the nuclei of non-permissive Sf9 cells that support a high titer of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) multiplication. Here we show, using the syncytium formation assay, that low-pH-triggered membrane fusion of BmNPV GP64 protein (Bm-GP64) is significantly lower than that of AcMNPV GP64 protein (Ac-GP64). Mutational analyses of GP64 proteins revealed that a single amino acid substitution between Ac-GP64 H155 and Bm-GP64 Y153 can have significant positive or negative effects on membrane fusion activity. Studies using bacmid-based GP64 recombinant AcMNPV harboring point-mutated ac-gp64 and bm-gp64 genes showed that Ac-GP64 H155Y and Bm-GP64 Y153H substitutions decreased and increased, respectively, the multiplication and cell-to-cell spread of progeny viruses. These results ...

2010-09-01

390

Treatment and Course of Community-Acquired Pneumonia Caused by Atypical Pathogens

In this thesis a study is made of the of voltage characteristics and reactive power control in the transmission network of the Electric Power Systems (EPS) with static compensators of VARs (SCVs). The necessity of voltage and reactive power control and the application of SCVs as a solution means for three representative operation stages of the EPS, considering balanced operation conditions and fundamental frequency is analyzed: Operation in the presence of incremental voltage changes, quasi-stationary operation or steady state and behavior at the occurrence of big disturbances. The application of SCVs to systems of integral transmission is in general a closed loop control system, reason why the analysis of the interaction of the system EPS-SCV is considered as an essential part of the voltage and reactive power control study. Firstly, the response analysis of the control loop is made for incremental voltage changes, representing the power system by means of its equivalent of Thevenin ...

1987-07-01

391

British Library Electronic Table of Contents (United Kingdom)

Objetivo Estudiar la evolucion de un grupo de neumonias extrahospitalarias causadas por germenes atipicos (Mycoplasma pneumoniae, Legionella spp., Coxiella burnetii y Chlamydophila pneumoniae) en funcion del tratamiento empirico recibido. Pacientes y metodos Entre enero de 1996 y febrero de 2001 ingresaron en nuestra unidad 390 casos de neumonia, de los que 89 estaban causados por germenes atipicos. Los pacientes se dividieron retrospectivamente en 2 grupos segun el tratamiento empirico pautado: grupo A, al que se habia proporcionado cobertura frente a germenes atipicos (quinolonas o macrolidos), y grupo B, al que no se habia proporcionado dicha cobertura. Se estudio la evolucion segun las diferencias entre ambos grupos en la estancia hospitalaria, la resolucion radiologica, el reingreso e...

2006-01-01

392

The angiogram sign in pulmonary atelectasis studied with helical Computed Tomography. Incidence and value; Il segno dell'angiogramma nelle atelettasie polmonari studiate con Tomografia Computerizzata spirale. Incidenza e validita' semeiologica

Results of the Interlaboratory Exercise CSN/CIEMAT-100 Among Environmental Radioactivity Laboratories (Soil); Resultados del Ejercicio Interlaboratorios de Radiactividad Ambiental CSN/CIEMAT-00 (Suelo)

In this issue it has been investigated the yield of Helical CT in the study of lung vessels, the incidence of the angiogram sign and its actual value. July 1997 to December 1998, were studied 30 cases of pulmonary collapse of different origin. It was found 15 cases of passive collapse (10 from pleural effusion, 2 from diaphragmatic compression, 2 from traumatic pneumothorax, and 1 from isthmic aortic aneurysm), 12 cases of obstructive collapse (9 from bronchogenic carcinoma, 1 from mucoid obstruction, 1 from ilar lymphadenopathy, and 1 from mediastinal cancer), 2 cases of adhesive collapse and 1 case of round atelectasis. All the examinations were performed with the Helical technique during nonionic iodinated contrast agent injection (bolus, 300-350 mg/mL); it was used an automatic injector set at 2-3 mL/s. It was studied the images for the angiogram sign, that is hyperdense bands, usually longer than 2 cm, through the collapsed lung, which correspond to normal pulmonary vascular ...

1999-12-01

393

Rational protection of the quality of coastal waters by means of integrated, real-time management of the water environment; Proteccion racional de la calidad de las aguas costeras mediante la gestion integrada y en tiempo real del medio hidrico

The document describes the outcome of the CSN/CIEMAT-00 interlaboratory test comparison among environmental radioactivity laboratories. the exercise was organised according to the ISO-43 and the ISO/IUPAC/AOAC Harmonized Protocol for the proficiency testing of analytical laboratories. the test sample was a soil containing environmental levels of K-40, Ra-226, Ac-228, Sr-90, Cs-137, Cs-134, Pu (239-240) y Am-241. the Universidad Autonoma de Barcelona prepared the material and reported adequate statistical studies of homogeneity. The results of the exercise were computed for 30 participating laboratories, and their analytical performance was assessed using the u-score approach. A raised percentage of satisfactory laboratory performance has been obtained for all the analysis, being the best performance in gamma measurements. The exercise has drawn that several laboratories have difficulties in the evaluation of combined uncertainty, mainly in analysis involving radiochemical steps. The ...

2002-07-01

394

Radiofrequency ablation of hepatocellular carcinoma extended into the portal vein: Preliminary results

Before the implementation Water Framework directive, it was usual to forget that a good environment protection of the receiving waters needs a correct and coordinated operation of the subsystems of the water cycle, specially sewerage system, WWTP and receiving waters. This explains that most of the countries have focused their efforts in the treatment of dry weather flows forgetting the management of wet weather flows. Actually the idea that a sewerage system or a WWTP can not be planned or managed independently without considering the effects on the receiving waters is commonly accepted because not only each one of these systems must work correctly but also it is required a minimum impact in the receiving waters of the sewerage and WWTP overflows in dry and wet weather. All these links will affect the management strategy of the sewerage system (storm water detection tanks, gates, pumping stations, etc)., the interceptor, the WWTP and the receiving waters. Only an integral planning of ...

2005-07-01

395

British Library Electronic Table of Contents (United Kingdom)

SommarioScopo In questo studio riportiamo i dati relativi all'ablazione percutanea con Radiofrequenza (RF) di HCC e trombosi neoplastica dei vasi portali. Pazienti e metodi Dieci pazienti (pz) cirrotici, 7 maschi e 3 femmine, eta media 68 anni, con 10 noduli di HCC e trombosi neoplastica dei vasi portali sono stati sottoposti ad ablazione percutanea con RF del trombo neoplastico e dell'HCC. La RF veniva ritenuta efficace quando alla TC con mdc si osservava la necrosi completa dell'HCC e al Color Doppler si osservava la ricanalizzazione completa dei vasi portali. Risultati La necrosi completa dell'HCC e la ricanalizzazione completa dei vasi portali si osservava in 7 (70%) dei 10 pazienti. Nei rimanenti 3 (30%) la necrosi dell'HCC e la ricanalizzazione dei vasi portali non risultavano comple...

2009-01-01

396

Quality controls in radiotherapy. From experiences of the first course-debate; Controlli di qualita' in radioterapia. Dalle esperienze del primo corso-dibattito

Prevalence and Predisposing Factors for Bundle Branch Block in Patients Starting Dialysis

The document reports the main issues discussed during the first course-debate on quality controls in radiotherapy, held in Rome in December 3-4, 1998 and May 3-5 1999 in collaboration between the Istituto Superiore di Sanita' and the Istituto Regina Elena. The points considered critical to the quality assurance in radiotherapy by the participants are presented in relation to the total radiotherapy procedure. [Italian] Questo documento e' dedicato al primo corso-dibattito sui controlli di qualita' in radioterapia svoltosi a Roma il 3-4 dicembre 1998 e il 3-5 maggio 1999 in collaborazione tra l'Istituto Superiore di Sanita' e l'Istituto Regina Elena. Esso presenta gli argomenti ritenuti critici per l'assicurazione di qualita' in radioterapia in relazione all'intera procedura radioterapica, in base alle esperienze dei partecipanti.

2000-07-01

397

British Library Electronic Table of Contents (United Kingdom)

Introduccion y objetivos Aunque los bloqueos de rama (BR) se consideran hallazgos frecuentes, la informacion sobre su prevalencia real en la poblacion general es escasa, y es inexistente en los pacientes en dialisis. El objetivo es establecer la prevalencia del BR completo en la poblacion que inicia dialisis y los factores relacionados con su aparicion. Como analisis adicional, se explora su asociacion con la mortalidad y con la aparicion de un evento cardiovascular. Metodos Se incluyo a los pacientes que iniciaron dialisis en nuestro centro entre el 1 de noviembre de 2003 y el 31 de diciembre de 2006. Todos fueron sometidos a valoracion cardiologica al inicio de dialisis. Determinamos el BR y su relacion con factores clinicos y parametros ecocardiograficos y bioquimicos. Los pacientes fue...

2008-01-01

398

New technology for the emulsification of petroleum residuals in water; Nueva tecnologia para la emulsificacion de residuales del petroleo en agua

Malignant pulmonary lesion. Possible causes of CT misdiagnosis; Possibilita' di falsi negativi nella diagnosi delle neoplasie polmonari con Tomografia Computerzzata

It turns out of interest to Comision Federal de Electricidad and Petroleos Mexicanos to reduce costs of fuel oil, reducing the viscosity of the residues petroleum of high vacuum distillation emulsifying these with water. The parameters that define the characteristics of the emulsions are mainly the relation of their components, viscosity, size and drop distribution and its stability. The experimental work began producing emulsions in form of lots, using samples of approximately 500 milliliters of vacuum residue. In this first stage, at different concentrations, were examined eight different surfactants, as well as combinations among them, also seven different stabilizers were used. With these tests it has been managed to identify the concentrations, as well as the most adequate relations of surfactants and stabilizers for the production of emulsions of vacuum residues in water. The size and drop distribution has been determined by means of an optical LEICA microscope, model DMLS, that ...

2001-07-01

399

Is intraoperative ultrasound (IOUS) still useful for the detection of liver metastases?

The purpose of this work is to describe the radiological appearance of overlooked malignant pulmonary lesions at CT and to analyze the reasons of misdiagnosis. Ten patient with pulmonary lesion (PML) overlooked at forst CT examination were selected among patients with lung cancer. Endobronchial location of the tumors and their small size were the most frequent causes of misdiagnosis of PML at chest CT in the series. However, a systematic evaluation of CT scans can reduce the percentage of missed lesions. [Italian] Scopo del presente lavoro e' di descrivere le caratteristiche radiologiche delle lesioni polmonari maligne non identificate con la Tomografia Computerizzata (TC) e definire le possibili cause di errore. Sono stati esaminato 10 pazienti con tumore polmonare. Le sedi endobronchiale e centrale nonche' le piccole dimensioni delle lesioni polmonari maligne rappresentano le cause piu' frequenti di errore diagnostico della TC. La ...

1999-06-01

400

British Library Electronic Table of Contents (United Kingdom)

SommarioScopo Valutare limpatto clinico negli anni dellecografia intraoperatoria (IOUS) nella identificazione delle metastasi epatiche rispetto alle altre metodiche di imaging. Materiali e metodi risultati delle ecografie intraoperatorie effettuate per lidentificazione delle metastasi epatiche in un periodo compreso tra gli anni 2000 e 2006 sono stati retrospettivamente revisionati e comparati con le metodiche di imaging preoperatorio: Ecografia (US), Tomografia Computerizzata (TC) e/o Risonanza Magnetica (RM). Tutti i casi sono stati patologicamente provati. Sono stati calcolati per anno i casi discordanti in termini di presenza/assenza di lesione focale epatica. Il confronto statistico e stato effettuato con il test di McNemar. Un valore del p<0,05 e stato considerato statisticamente sig...

2009-01-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

401

Estimation of the renewable power resource (Chapter 2 in 'A vision of year 2030 on the use of the renewable energies in Mexico'); Estimacion del recurso energetico renovable (Capitulo 2 en 'Una vision al 2030 de la utilizacion de las energias renovables en Mexico')

Electrochemical treatment of olive oil mill wastewater

Most of the information in this chapter is obtained from 1, 2, 3, 6, 8 and 9 annexes. It is worthwhile to clarify that in most of the cases they are estimations of the available resources, such as: trash, geothermal, solar energy, wind energy, biomass and mini and micro-hydro-energy. In several cases punctual measurements have been made, which do not represent a generic value of a national reserve. [Spanish] La mayor parte de la informacion en este capitulo es obtenida de los anexos 1, 2, 3, 6, 8 y 9. Cabe aclarar que en la mayoria de los casos son estimaciones de los recursos disponibles, tales como: la basura, la geotermia, la energia solar, la energia eolica, la biomasa y, la mini y la micro-hidroenergia. En varios casos se han realizado mediciones puntuales, las cuales no representan un valor generico de una reserva nacional.

2005-08-15

402

Effect of water purification process in radioactive content: analysis on small scale purification plants; Efecto del proceso de purificacion de agua en el contenido radiactivo: analisis en plantas purificadoras a pequena escala

The possibility of oxidizing at a PbO{sub 2} anode the phenols and polyphenols, present in the olive oil mill wastewater, has been studied as a pre-treatment for the submission of such wastewater to the traditional biological treatments. The results obtained operating at current densities ranging 500 to 2000 A/m{sup 2} show that it is possible to reduce the concentration of the phenolic components, which interfere with the biological treatments, down to low values without decreasing too much the total organic content of the wastewater. [Italian] E' stata studiata la possibilita' di ossidare anodicamente i componenti fenolici delle acque reflue di frantoio, quale pretrattamento delle stesse prima del loro invio ai processi di trattamento biologico. I risultati ottenuti impiegando PbO{sub 2} quale materiale anodico e operando con densita' di corrente comprese tra 500 e 2000 A/m{sup 2} mostrano come sia possibile eliminare, o almeno ...

2001-04-01

403

Effect of the steam explosion pretreatment on enzymatic hydrolysis of eucalyptus wood and sweet sorghum baggages; Efecto del pretratamiento con explosion por vapor en la hidrolisis enzimatica de madera de eucalipto y bagazo de sorgo

Water from small scale purification plants is a low cost alternative for consumers in comparison to the bottled commercial presentations. Because of its low cost per liter, the consumption of this product has increased in recent years, stimulating in turn the installation of purification systems for these small businesses. The purpose of this study was to estimate the efficiency of small scale purification systems located in the cities of Zacatecas and Guadalupe, Zacatecas, to reduce the radioactive content of water. It was measured the total alpha and beta activity in water samples of entry and exit to process, through the liquid scintillation technique. In general it was observed that the process is more efficient in removing alpha that beta activity. The fraction of total alpha activity removed varied between 27 and 100%, while between 0 and 77% of the total beta activity was removed by the analyzed plants. In all cases, the total radioactivity level was lower than the maximum ...

2009-10-15

404

Durability of bare and anodised aluminium in atmosphere of very different corrosivities I. Bare aluminium; Durabilidad del aluminio desnudo y anodizado en atmosferas de muy diferentes corrosividades. I. Aluminio desnudo

The effect of steam explosion treatment on the enzymatic hydrolysis yield of two different lignocellulosic substrates is studied. Raw materials have been pretreated in a pilot plant designed to work in batch and equipped with a reactor vessel of 2 1 working volume where biomass was heated at the desired temperature and then exploded and recovered in a cyclone. Temperatures from 190 to 230 degree celsius and reaction times from 2 to 8 min. have been assayed. The efficiency of the steam explosion treatment has been evaluated on the composition of the lignocellulosic materials as well as on their enzymatic hydrolysis yield using a cellulolytic complex from T. reesel. Results show a high solubilization rate of hemicelluloses and variable losses of cellulose and lignin depending on the conditions tested. Enzymatic hydrolysis yields of both substrates experimented remarkable increments, corresponding the highest values obtained to 210 degree celsius; 2 min. and 21O degree celsius; 4 min. for ...

1991-07-01

405

Determination of the hydrolysis constants of Europium (III), in ion strength media 4, 5 and 6 M NaClO{sub 4} at 303 K; Determinacion de las constantes de hidrolisis del Europio (III), en medios de fuerza ionica 4, 5 y 6 M de NaClO{sub 4} a 303 K

The behaviour of bare aluminium is studied in atmospheric exposure at 11 natural testing stations with salinity levels ranging between 2.1 and 684 mg Cl''- m''-2 d''-1. In atmospheres of low or moderate aggressivity aluminium behaves as a passive material, though the insignificant corrosion that is produced is sufficient to spoil its appearance. In contrast, at salinity levels of 50 mg Cl''- m''-2 ''-1 or above, aluminium is susceptible to pitting corrosion even in the first year of atmospheric exposure, or in the second year at salinities of {<=} 10 mg Cl''- m''-2 d''-1. For comparative purposes, results are included for aluminium protected with an anodic film of 28 {mu}m thickness exposed at the same testing stations. A 28 {mu}m anodic film, correctly sealed, prevents the risk of localised corrosion even in the most ...

2004-07-01

406

Degradation of recalcitrant organic pollutants by mixed catalysts; Degradazione di inquinanti organici recalcitranti mediante catalizzatori misti

This work was made with the purpose to complete information about the hydrolysis constants of Europium (III) in high ion strength media. So it was determined at a ion forces media 4, 5 and 6 M of sodium perchlorate at 303 K. The method used was the potentiometric with the aid of the Super quad computer program. In high ion strength media, the measurements of p H do not correspond directly to negative logarithm of the concentration of hydrogen ions, by this it is necessary to calibrate the electrode in these conditions. The Europium was hydrolized at pC{sub H} values greater 6 in all cases. The potentiometric method used under the described experimental conditions is adequate to determine the hydrolysis constants of Europium (III). According to the results and diagrams of chemical species of Europium obtained we can conclude that the hydrolysis constants, differ by its distribution but not in its identity. (Author)

1999-07-01

407

Constructing Folksonomies from User-specified Relations on Flickr

The photodegradation of two well known recalcitrant compounds was studied. At this aim, a photosystem constituted by titanium dioxide and polyaniline, both immobilised in a PVC membrane, and a sunlight simulating irradiation source was used. The optimisation of the experimental conditions, ph, ionic strength and, when operating on soils, humic acid concentration, allowed the photodegradation of Paraquat at a nearly 100% extent, while, an oxidation by hypochlorite was needed to degrade cyanuric acid. [Italian] E' stata studiata la fotodegradazione di due composti notoriamente recalcitranti a questo tipo di rimozione. Con un sistema fotocatalitico costituito da biossido di titanio e polianilina immobilizzati in una membrana di PVC ed operando con luce solare simulata e' possibile, ottimizzando le condizioni sperimentali di pH, forza ionica e - operando su terreno - concentrazione di acidi umici, ottenere la degradazione del paraquat fino a valori ...

2001-05-01

408

CT findings after normal and unsuccessful functional endoscopic sinus surgery; Ruolod della tomografia computerizzata dopo chirurgia funzionale dei seni paranasali: rilievi normali e valutazioni degli insuccessi chirurgici

Many social Web sites allow users to publish content and annotate with descriptive metadata. In addition to flat tags, some social Web sites have recently began to allow users to organize their content and metadata hierarchically. The social photosharing site Flickr, for example, allows users to group related photos in sets, and related sets in collections. The social bookmarking site Del.icio.us similarly lets users group related tags into bundles. Although the sites themselves don't impose any constraints on how these hierarchies are used, individuals generally use them to capture relationships between concepts, most commonly the broader/narrower relations. Collective annotation of content with hierarchical relations may lead to an emergent classification system, called a folksonomy. While some researchers have explored using tags as evidence for learning folksonomies, we believe that hierarchical relations described above offer a high-quality source of evidence ...

2008-01-01

409

CT diagnosis of subphrenic abscess after splenectomy for trauma; Ruolo della tomografia computerizzata nella valutazione degli ascessi subfrenici dopo splenectomia post-traumatica

The paper investigates the functional endoscopic sinus surgery with the Messer-linger's technique which treats benign or inflammatory diseases of paranasal sinuses resistant to medical therapy. The goal of this type of surgery is to open the obstructed sinus ostia and restore normal aeration and mucociliary clearance. The computerized tomography permits an accurate assessment of extension and results of functional endoscopic sinus surgery. [Italian] L'articolo discute l'uso della tecnica di Messerklinger nella chirurgia funzionale endoscopica dei seni paranasali per il trattamento delle malattie benigne e delle infiammazioni croniche. Essa disostruisce gli osti sinusali ripristinando la normale areazione e la pulizia mucociliare. La tomografia computerizzata consente di valutare l'estensione e i risultati del trattamento chirurgico funzionale, soprattutto nel caso di insuccesso terapeutico, rilevando malattia nasosinusale ...

1999-09-01

410

Black tide from the Bay of Campeche. [June 3, 1976 blowout

The paper studies subphrenic inflammatory abscesses and splenic fluid collections after splenectomy for trauma. These complications may appear early or late postoperatively; they are easily demonstrated with computerized tomography, which permits accurate spatial assessment of the lesions and appropriate treatment with percutaneous drainage. It has been also investigates the diagnostic accuracy of the CT in subphrenic inflammatory conditions after emergency splenectomy for traumatic spleen rupture and found that CT is a precious tool for rapid and easy diagnosis and follow-up of subphrenic abscesses treated with percutaneous drainage. [Italian] Scopo del lavoro e' di valutare il ruolo della tomografia computerizzata nelle flogrosi subfreniche secondarie a splenectomia d'urgenza per rottura traumatica della milza, documentando come tale metodica risulti accurata nel diagnosticare e monitorare in modo semplice e rapido l'evoluzione e il ...

1999-09-01

411

Behaviour of OFHC copper in highly pure water; Comportamento del rame OFHC in acqua di elevata purezza

The ruptured offshore oil well 80 miles from Mexican town of Ciudad del Carmen, which poured 8000 barrels of light oil a day into the Gulf of Mexico, provided marine scientists with the opportunity to monitor the spill and see what happens to petroleum hydrocarbons in sea water. Scientists followed changes in viscosity, surface tension, and buoyancy of individual oil globs as they moved through the water and interacted with the surface slick. Using gas chromatography and mass spectroscopy, chemists are gaining an understanding of the unique characteristics of the oil. Computer-generated models projected the slick's movement in the Gulf in response to wind and wave pressures, but the sophisticated system did not give an accurate daily prediction. Immediate damage to fish, turtles, and sea birds was documented by tagging. Long-term effects will take longer to ascertain since 25 years is generally required to flush oil out of sediments. Fishermen do not agree ...

1980-01-24

412

Anode Front-End Electronics for the Cathode Strip Chambers of the CMS Endcap Muon Detector

In order to gain a wider knowledge of corrosion phenomena in OFHC (oxygen free high conductivity) copper pipes of the stator cooling circuit of a power plant turboalternator, a research program was laid out which is based on the use of an experimental loop simulating real operating conditions as closely as possible. The samples examined were either copper foils or hollow conductors of the type that is normally used in the stator cooling system, and the circulating fluid was high purity water with controlled O/sub 2/, H/sub 2/ and C0/sub 2/ contents. Investigations were focused on the kinetics of O/sub 2/ removal through catalytic resins covered with metallic palladium (at different temperatures and pressures) and on the kinetics of CO/sub 2/ removal through mixed bed resins. Subsequently, attention was directed to the corrosion of copper (in the form of foils or hollow conductors) under different experimental conditions simulating either optimal or anomalous situations that can occur ...

1991-12-31

413

Anatomic and radiologic correlations in spontaneous hematomas of the rectus abdomninis muscles; Correlazioni anatomo-radiologiche negli ematomi spontanei dei muscoli retti dell'addome

The front-end electronics system for the anode signals of the CMS Endcap Muon cathode strip chambers has about 183000 channels. The purposes of the anode front-end electronics are to acquire precise muon timing information for bunch crossing number identification at the Level-1 muon trigger system and to provide a coarse radial position of the muon track. Each anode channel consists of an input protection network, amplifier, shaper, constant-fraction discriminator, and a programmable delay. The essential parts of the electronics include a 16-channel amplifier-shaper-discriminator ASIC CMP16 and a 16-channel ASIC D16G providing programmable time delay. The ASIC CMP16 was optimized for the large cathode chamber size (up to 3 x 2.5 m2) and for the large input capacitance (up to 200 pf). The ASIC combines low power consumption (30 mW/channel) with good time resolution (2 - 3 ns). The del ay ASIC D16G makes possible the alignment of signals with an accuracy of 2.2 ns. ...

2005-01-01

414

Analytical surveys on water quality of Trasimeno Lake: Definition of trophic levels; Indagine sulla qualita` delle acque del Lago Trasimeno: Definizione dei livelli trofici

Rectus sheath hematomas are a frequent but sometimes misdiagnosed disease in patients under anticoagulative drugs, hemodialysis, or simply in the elderly. The most frequent localization is in the lower part of the abdomen: the explanation lies in the anatomy of the abdominal wall, especially in the arcuate line of the rectus sheath. Aim of this work is to explain the reason of the almost constant location correlating the anatomy with the CT features. The rectus abdominis muscle lies between the aponeuroses of the transverse and oblique muscles which form the so called rectus sheath. This arrangement is found from the costal arch to a level approximately between the umbilicus and the pubic symphisis, where the rear layer of the rectus sheath ends with a curved edge, called the arcuate or semicircular line of Douglas. Beneath this line the aponeuroses of the three muscles pass in front of the rectus which is separated from the peritoneum only by the fascia trasversalis, a thin connective ...

2000-06-01

415

A method for the optimal design of networks of drinking water distribution; Un metodo para el diseno optimo de redes de distribucion de agua potable

The previous judgment of eutrophic state, once proposed for the Trasimeno Lake (Umbria region, Italy), has been revised. According to Vollenweider-OECD classification criterion, the lake waters are at present to be classified as mesotrophic waters, with phosphorus limitation. However this level of moderate productivity results to be conditioned by the hydrochemical characteristics of the lake waters, that control phosphorus concentrations independently from the loading coming from the basin, and prevent dangerous and massive algal blooms. Different processes of chemical nature, regulating the phosphorus amount in the lake waters, are then reviewed: adsorption of P/P0(4) onto suspended materials, particularly clay mineral particles, and direct precipitation as hydroxylapatite or co-precipitation with carbonates, are found to be the principal mechanisms that determine chemical removal of phosphorus from the water column. A preliminary mass balance of phosphorus subtracted from the system ...

1995-06-01

416

Osteoporosis and prevention. Assessment of mineral density, geometry and biomechanics of bone by means of peripheral quantitative Computed Tomography (pQCT) in premenopausal women assuming phytoestrogens; Osteoporosi e fitoestrogeni: valutazione della densita' minerale ossea mediante tomografia computerizzata quantitativa periferica nelle donne lattoovovegetariane nella premenopausa

A method to select, among several commercial diameter options, the most economic combination of pipeline networks is presented. These networks comply with the discharge conditions in the pipes and pressure in the knots. It is applied a static method for the hydraulics analysis of the Engineering Institute of the Universidad Nacional Autonoma de Mexico (UNAM) and an optimization technique to reduce the number of calculations. [Spanish] Se presenta un metodo para seleccionar, de entre varias opciones de diametros comerciales, la combinacion que forma la red de tuberias mas economica que satisface las condiciones de gasto en los tubos y de presion en los nudos de la misma. Para el analisis hidraulico se emplea un metodo estatico del Instituto de Ingenieria de la Universidad Nacional Autonoma de Mexico (UNAM) y se aplica una tecnica de optimacion para reducir el numero de calculos y alcanzar la solucion.

1998-12-01

417

Integration of SPECT with CT to plan radiotherapy fields. n application to conformal irradiation for lung carcinoma; Integrazione delle immagini della Tomografia Computerizzata con emissione di fotone singolo in un sistema commerciale per l'elaborazione dei piani di radioterapia. Applicazione all'irradiazione conformazionale del carcinoma polmonare

Aim of the work was to describe the noninvasive assessment of bone mineral density, geometrical and biochemical properties in premenopausal women with dietary intake of phytoestrogens and comparison of these parameters with those of age-matched female subjects with Mediterranean dietary intake lacking in these substances. Volumetric cortical, trabecular and total mineral density and bone geometrical properties were evaluated with peripheral quantitative computed tomography (pQCT) at the distal radius of the non dominant forearm. pQCT showed higher bone mineral density (total and trabecular) and SSI values in premenopausal with dietary intake of phytoestrogens. Despite the lack of statistical significance, these preliminary results, should further support the few literature findings about the potential role of phytoestrogens consumption in preventing trabecular bone loss. However, further studies are warranted to evaluate definitively postmenopausal osteoporosis. [Italian] Scopo ...

2000-04-01

418

Gunshot wounds to the head. Correlations between CT and clinical findings, neurosurgical treatment and long-term sequels; Traumi cranio-encefalici da proiettile: correlazione tra tomografia computerizzata, clinica, trattamento neurochirurgico e sequele a distanza

Single photon emission computed tomography (SPECT) of lung perfusions permits to map functioning lung parenchyma with higher sensitivity than CT. Delivering higher radiations doses it used to increase local control in lung carcinoma; this strategy is based on radiobiological and clinical studies. Lung parenchyma is a dose-limiting tissue in patients irradiated for lung cancer. Functional mapping based on SPECT and CT findings permits to design radiation beams such as to minimize irradiation of functioning lung. [Italian] L'integrazione delle immagini diagnostiche aventi diverso contenuto informativo anatomico-funzionale puo' essere utilizzata nella radioterapia per migliorare le procedure diagnostiche e ottimizzare la geometria dell'irraggiamento. I sistemi per l'elaborazione di piani di radioterapia oggi in uso sono direttamente interfacciati con gli apparecchi di Tomografia Computerizzata (TC) e l'impiego di immagini provenienti da altre ...

1999-04-01

419

Multiphasic helical CT of hepatocellular carcinoma. Evaluation after chemoembolization; Tomografia Computerizzata spirale multifasica dell'epatocarcinoma. Valutazione dopo chemioembolizzazione

With the aim of demonstrating the usefulness of computerised tomography (CT) findings in the planning of brain neurosurgery in gunshot victims, for prompt and successful treatment, 30 patients with brain gunshot wounds were examined with CT over 5 years. The mortality rate of firearm wounds of the skull base was 34% higher than that of the hemisphere; this is due to carotid hemorrhage and midbrain damage. Such traumas require emergency radiological diagnosis and neurosurgical treatment because of their severity and early irreversible complications. Complex operations and skilled surgeons may prevent disabling postoperative sequels. CT findings are indispensable and must be correctly interpreted. The radiologist and the neurosurgeon must collaborate closely and both must consider several diagnostic factors affecting surgical planning. [Italian] Scopo dello studio e' stato quello di dimostrare l'utilita' della semeiologia della tomografia computerizzata in ...

2000-03-01

420

CT in the study of thoracic-pulmonary complications after liver transplantation; La Tomografia Computerizzata nello studio delle complicanze toraco-polmonari dopo trapianto del fegato

The main purpose of this work is to report the personal experience with addition of contrast-enhanced multiphase helical CT to unenhanced CT (Lipiodol CT) in the evaluation of patients with hepatocellular carcinoma treated with chemoembolization and to analyze the present role of oily agent CT. It has been retrospectively reviewed the examinations of 42 consecutive patients submitted to global chemoembolization over a 2-year period. CT was performed 18-30 days after the treatment. The Lipiodol CT study was carried out with volume acquisitions. It has been considered as nodules all well-defined areas with dense oily agent uptake; uptake itself was classified as: 0=absent, I=lower than 10% of the tumor volume; II=lower than 50%, III=50%, IV=homogeneous. Contrast-enhanced helical CT was performed with the 2-phase technique in 28 patients and with the 3-phase technique in 14; it has been considered as nodules all well-defined and relatively homogeneous areas with hyper attenuation in the ...

2000-06-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

421

Role of Spiral and Multislice Computed Tomography in the evaluation of traumatic and spontaneous oesophageal perforation. Our experience; Ruolo della Tomografia Computerizzata Spirale e Multistrato nello studio delle lesioni traumatiche e spontanee dell'esofago: nostra esperienza

In this article is reviewed the role of Computed Tomography (CT) in thoracic complications following ortho topic liver transplantation (OLT). In a post-OLT population of 567 patients transplanted in the Italian institutions, 100 patients (17.6%) were examined with chest CT. It was reviewed data relative to the total number of examinations, clinical and/or radiographic indications, the CT technique-i.e., conventional (with (out) intravenous, i.v., contrast material) or high-resolution (HRCT). It also reviewed the radiologic patterns and their correlation with the other clinical, bronchoscopic and/or laboratory results. Of 152 chest CT examinations, 45 (29.6%) were performed because of clinical indications, 31 (20.4%) because of a radiographic abnormality, 64 (42.1%) because of clinical and radiographic abnormality, 64 (42.1%) because of clinical and radiographic indications, while in 12 cases (7.9%) the reasons were unknown; 133/152 (87.5%) examinations had been performed with ...

2000-12-01

422

Strategies to optimize the outcome of children given T-cell depleted HLA-haploidentical hematopoietic stem cell transplantation

Purpose: To assess the role of CT in the evaluation of traumatic and spontaneous oesophageal perforation. Materials and methods: From March 2001 to May 2003, we studied 12 patients (7 males and 5 females; age range: 25-66 years, mean age: 43.5 years) with suspected oesophageal perforation due to motor-vehicle accidents (4 cases), stab wound (one case), post-intubation (2 cases), foreign body ingestion (2 cases) and spontaneous (3 cases). Five patients underwent standard chest and cervical radiography; two patients with suspected foreign body ingestion also underwent a gastrografin swallow study; all of the 12 patients underwent CT of the neck, chest and abdomen before and after intravenous, and in four cases oral, administration of contrast material. Results: In 5 patients with cervical, thoracic and abdominal trauma, the CT examination showed the presence of pleuroparenchymal injury (pneumothorax, pleural effusion and subcutaneous emphysema) as well as findings suggestive of ...

2005-03-01

423

British Library Electronic Table of Contents (United Kingdom)

The most advanced frontier of allogeneic hematopoietic stem cell transplantation (allo-HSCT) is represented by the use of an HLA-partially matched relative as donor. In this type of transplantation, donor-derived natural killer (NK) cells, which are alloreactive towardtoward recipient cells, significantly contribute to the eradication of leukemia blasts. Alloreactive NK cells may also kill host dendritic cells and T lymphocytes, thus preventing graft-versus-host disease and graft rejection, respectively. Sophisticated strategies of adoptive infusion of T-cell lines/clones specific for the most life-threatening pathogens (namely cytomegalovirus, Epstein-Barr virus, Aspergillus and Adenovirus) have been envisaged, and successfully tested in a few pilot trials, to protect the recipient in the...

2011-01-01

424

Specialized Circuits from Primary Visual Cortex to V2 and Area MT

British Library Electronic Table of Contents (United Kingdom)

SummaryPrimary visual cortex recombines inputs from magnocellular (M) and parvocellular (P) streams to create functionally specialized outputs. Understanding these input-output relationships is complicated by the fact that layer 4B, which provides outputs to dorsal visual areas, contains multiple cell types. Using a modified rabies virus that expresses green fluorescent protein, we show that layer 4B neurons projecting to MT are a majority spiny stellate, whereas those projecting to V2 are overwhelmingly pyramidal. Regardless of cell type, MT-projecting neurons have larger cell bodies, more dendritic length, and are deeper within layer 4B. Furthermore, MT-projecting pyramidal neurons are located preferentially underneath cytochrome oxidase blobs, indicating that MT-projecting neurons of bo...

2007-01-01

425

Serological and virological survey and resighting of marked wild geese in Germany

British Library Electronic Table of Contents (United Kingdom)

In order to investigate the potential role of arctic geese in the epidemiology, the spatial and temporal spread of selected avian diseases, in autumn 2002, a virological and serological survey designed as capture-mark-resighting study was conducted in one of the most important coastal resting sites for migratory waterfowl in Germany. Oropharyngeal, cloacal swabs and blood samples were collected from a total of 147 birds comprising of three different arctic geese species including White-fronted Goose (Anser albifrons), Tundra Bean Goose (Anser fabalis rossicus), Pink-footed Goose (Anser brachyrhynchus) as well as from 29 non-migratory Canada Geese (Branta canadensis). Altogether, six adeno-like viruses (ALV; 95% CI, 1.74?9.92%) and two avian paramyxoviruses (APMV-4; 95% CI, 0.19?5.53%) were...

2011-01-01

426

Review on the immunology of European sea bass Dicentrarchus labrax

British Library Electronic Table of Contents (United Kingdom)

European sea bass (Dicentrarchus labrax L.) is a marine species of great economic importance, particularly in Mediterranean aquaculture. However, numerous pathogenic viruses, bacteria, fungi and parasites affect the species, causing various infectious diseases and thereby leading to the most heavy losses in aquaculture production of sea bass. In this respect, knowledge on molecular and genetic mechanisms of resistance to pathogens and specific features of immune response against various infectious agents should greatly benefit the development of effective vaccines and proper vaccination strategies in marker-assisted selection of fish resistant to a range of infections. To date, genetic knowledge on sea bass immune regulatory genes responsible for resistance to pathogens is relatively poor ...

2007-01-01

427

Response to measles-mumps-rubella vaccine in children on dialysis.

Prostate cancer immunology - an update for Urologists

Ten children receiving maintenance dialysis were immunized with the standard dose of measles-mumps-rubella vaccine between 15 and 33 months of age. Immune responses to vaccination were determined using commercially available enzyme-linked immunosorbent assays for measles, mumps, and rubella viruses. Eight children responded to measles vaccine, 5 to mumps vaccine, 8 to rubella vaccine, and only 3 children to all three vaccines, compared with a seroconversion rate of over 90% to all three vaccines in healthy children (P less than 0.0001). We contend that the relatively poor immunocompetence of our dialysis patients explains their less than optimal vaccine response and suggest that children vaccinated while undergoing dialysis be tested to confirm serological evidence of immunity. PMID:1571219

1992-03-01

428

British Library Electronic Table of Contents (United Kingdom)

A better understanding of the immune processes in the pathogenesis and progression of prostate cancer (CaP) may point the way towards improved treatment modalities. The challenge is to amplify immune responses to combat tumour escape mechanisms. Infection and inflammation may have a role in prostate carcinogenesis, including the newly discovered xenotropic murine leukaemia virus (XMRV). These inflammatory states damage defence mechanisms and induce a high proliferative state favouring further mutation and impaired immune surveillance. With this knowledge we are able to explore the use of immunotherapy to rejuvenate the immune system in combating CaP. Recently Sipuleucel-T, an immunotherapeutic agent for metastatic androgen independent CaP, has resulted in improved survival and might be the...

2011-01-01

429

Probiotic-Induced Priming of Innate Immunity to Protect Against Rotaviral Infection

British Library Electronic Table of Contents (United Kingdom)

Understanding of probiotic-induced regulatory gene expression and networking is critical to further explore their roles in controlling infection. Transcriptional profile of selected innate immune genes in primary bovine intestinal epithelial cells was assessed over a time course of incubation with the probiotic Lactobacillus plantarum 299v. Based on gene expression results, a time point was chosen to prime epithelial cells with the probiotic prior to infection with rotavirus. Plaque assays and genomic analysis provided the basis for establishing the efficacy of probiotics in preventing a rotaviral infection. Plaque assays revealed that the probiotic is capable of decreasing (at least by 100-fold) the levels of live virus when the cells were primed with the probiotic. Results from gene expr...

2010-01-01

430

Preliminary crystallographic analysis of a possible transcription factor encoded by the mimivirus L544 gene

British Library Electronic Table of Contents (United Kingdom)

Mimivirus is the prototype of a new family (the Mimiviridae) of nucleocytoplasmic large DNA viruses (NCLDVs), which already include the Poxviridae, Iridoviridae, Phycodnaviridae and Asfarviridae. Mimivirus specifically replicates in cells from the genus Acanthamoeba. Proteomic analysis of purified mimivirus particles revealed the presence of many subunits of the DNA-directed RNA polymerase II complex. A fully functional pre-transcriptional complex appears to be loaded in the virions, allowing mimivirus to initiate transcription within the host cytoplasm immediately upon infection independently of the host nuclear apparatus. To fully understand this process, a systematic study of mimivirus proteins that are predicted (by bioinformatics) or suspected (by proteomic analysis) to be involved in...

2011-01-01

431

Opportunistic esophagitis in AIDS: Radiographic diagnosis

No detectable XMRV in subjects with chronic fatigue syndrome from Quebec

Between 1983 and 1986, 35 of 90 patients with acquired immunodeficiency syndrome (AIDS) had double-contrast esophagograms to rule out opportunistic esophagitis. The radiographs were reviewed without knowledge of the clinical or endoscopic findings. Candida esophagitis was diagnosed radiographically in 17 patients who had varying degrees of plaque formation and viral esophagitis in three who had discrete ulcers without plaques. All three patients with viral esophagitis (herpes in 2 and cytomegalo virus in one) and 15 of 17 with Candida esophagitis had endoscopic and/or clinical corroboration of the radiographic diagnosis. Thus, the authors' experience suggests that fungal and viral esophagitis can often be differentiated on double-contrast esophagography, so that appropriate antifungal or antiviral therapy can be instituted without need for endoscopic intervention.

432

British Library Electronic Table of Contents (United Kingdom)

We investigated the presence of XMRV in a cohort of Quebec patients with chronic fatigue syndrome (CFS). DNA was purified from activated peripheral blood mononuclear cells (PBMCs) and PCR was used to detect XMRV gag and env in 72 patients. Anti-XMRV antibodies were searched in sera of 62 patients by Western blot analysis. Attempts to detect XMRV antigens was made, using immunofluorescence with Gag anti-p30 antiserum on activated PBMC from 50 patients. Plasma viremia was measured by RT-PCR on 9 subjects. Finally, detection of infectious virus in 113 CFS subjects was made by co-culture of PHA+IL-2 activated PBMC with human LNCaP carcinoma cells, and by infecting the same susceptible cells with plasma, using a reverse transcriptase (RT) assay as a readout in both experiments. No detection of ...

2011-01-01

433

Involvement of the Tpl-2/cot oncogene in MMTV tumorigenesis.

Hepatocellular Carcinoma (HCC)

We report for the first time a relationship between the Tpl-2/cot oncogene and Mouse Mammary Tumor Virus (MMTV) associated transformation of mammary gland cells. A sub-genomic library generated from a primary mammary gland tumor yielded a novel MMTV integration site which disrupted the Tpl-2/cot proto-oncogene between exons 7 and 8. Comparison of a cell line derived from normal mammary gland (comma-D) and a cell line established from an MMTV induced mammary tumor (GR) demonstrated similar rearrangements within Tpl-2/cot for the GR cells but not in the comma-D cells. These rearrangements in the cell line were accompanied by an increase in the level of Tpl-2/cot specific mRNA. This data suggests that Tpl-2/cot expression may be important in epithelial cell transformation or tumor progression. PMID:8934549

1996-11-01

434

Hepatitis B and C in dialysis units in Iran: Changing the epidemiology

Hepatocellular carcinoma (HCC) is considered to be one of the most common malignancies worldwide, and the most common one in Africa and Asia. Over the last decade, a rising incidence of up to 10-15/100,000 per population has been seen in the Western world, with an estimate of 250,000 deaths and more than a million worldwide per year. By the year 2010, the World Health Organization expects that HCC will be the leading cause of cancer mortality surpassing lung cancer. This increasing incidence is most likely related to an increasing prevalence of chronic hepatitis C (HC) and B (HB) virus infections and other diseases inducing chronic inflammation (Befeler and Di Bisceglie 2002; Llovet et al. 2003).

2006-01-01

435

British Library Electronic Table of Contents (United Kingdom)

Abstract Hepatitis B (HBV) and C (HCV) viruses are the most important infections transmitted by the parenteral route in patients receiving maintenance dialysis. The prevalence varies markedly from country to country. The aim of this study is to review the efficacy of the strategies to reduce the incidence of these infections and the trend of results in Iran. As a routine, all hemodialysis patients in Iran have biannual blood samples for assessment of serum HBSAg, HBS Abs, and HCV Abs. The data are collected in the Ministry of Health. For statistical analysis, prevalence, and incidence were calculated. There is an increasing prevalence/incidence of end-stage renal disease (ESRD) in Iran, from 238/49.9 pmp in 2000 to 357/63.8 pmp in 2006. The prevalence of positive HBSAg and HCV Abs decrease...

2008-01-01

436

Follow-up of pregnant women exposed to chicken pox: an audit of relationship between level of antibody and development of chicken pox

British Library Electronic Table of Contents (United Kingdom)

The purpose of this study was to validate through natural exposure a cut-off level of varicella zoster IgG as protective against infection with varicella zoster virus (VZV). Laboratory testing to determine VZV immune status of pregnant women exposed to varicella is recommended. Quantitative assays are now available which are sensitive and specific. More than 200 consecutive requests for screening in pregnant patients with recent varicella contacts were followed-up by questionnaire. DiaSorin LIAISON and VZV time resolved fluorescence immuno assay (VZV TRFIA) were used to measure VZV antibody level. One hundred fifty out of 209 (72%) questionnaires were returned; 14 patients developed varicella, 129 did not and seven were not known. Patients who had been given VZIG and developed varicella on...

2011-01-01

437

Effective Population Size Is Positively Correlated with Levels of Adaptive Divergence among Annual Sunflowers

British Library Electronic Table of Contents (United Kingdom)

The role of adaptation in the divergence of lineages has long been a central question in evolutionary biology, and as multilocus sequence data sets have become available for a wide range of taxa, empirical estimates of levels of adaptive molecular evolution are increasingly common. Estimates vary widely among taxa, with high levels of adaptive evolution in Drosophila, bacteria, and viruses but very little evidence of widespread adaptive evolution in hominids. Although estimates in plants are more limited, some recent work has suggested that rates of adaptive evolution in a range of plant taxa are surprisingly low and that there is little association between adaptive evolution and effective population size in contrast to patterns seen in other taxa. Here, we analyze data from 35 loci for si...

2011-01-01

438

Detecting retroviral sequences in chronic fatigue syndrome.

Channelrhodopsin-2 gene transduced into retinal ganglion cells restores functional vision in genetically blind rats

XMRV or xenotropic murine leukemia virus-related retrovirus, a recently discovered retrovirus, has been linked to both prostate cancer and chronic fatigue syndrome (CFS). Recently, the teams of Drs. Shyh-Ching Lo and Harvey Alter discovered the presence of sequences closely related to XMRV in the blood of 86.5% of patients with CFS [1]. These findings are important because since the initial discovery of XMRV in CFS, several studies have failed to find XMRV in specimens collected from CFS patients. While the current study also did not find XMRV in CFS, Lo et al. did detect sequences that belong to polytropic mouse endogenous retroviruses (PMV), which share considerable similarity with XMRV. Criteria for future studies that will help bring greater clarity to the issue of retroviral sequences in CFS are proposed below. PMID:21994623

2010-11-03

439

British Library Electronic Table of Contents (United Kingdom)

To test the hypothesis that transduction of the channelrhodopsin-2 (ChR2) gene, a microbial-type rhodopsin gene, into retinal ganglion cells of genetically blind rats will restore functional vision, we recorded visually evoked potentials and tested the experimental rats for the presence of optomotor responses. The N-terminal fragment of the ChR2 gene was fused to the fluorescent protein Venus and inserted into an adeno-associated virus to make AAV2-ChR2V. AAV2-ChR2V was injected intravitreally into the eyes of 6-month-old dystrophic RCS (rdy/rdy) rats. Visual function was evaluated six weeks after the injection by recording visually evoked potentials (VEPs) and testing optomotor responses. The expression of ChR2V in the retina was investigated histologically. We found that VEPs could not b...

2010-01-01

440

Benign lymphoepithelial lesion of the parotid gland in AIDS patients: CT characteristics

A novel glioblastoma cancer gene therapy using AAV-mediated long-term expression of human TERT C-terminal polypeptide

Contrast agent-enhanced CT scans in nine male patients with histologically proved benign lymphoepithelial lesions of the perotid gland were reviewed. All scans showed cystic-appearing masses with peripheral rim enhancement corresponding to the macroscopic appearance of the lesion. Five patients were seropositive for human immunodeficiency virus (HIV) infection or had infections seen in acquired immunodeficiency syndrome. Three patients were members of high-risk groups. Only one patient had symptoms of the SICCA syndrome. Once a rare cause of parotid gland enlargement, benign lymphoepithelial lesions have recently been seen with increasing frequency in patients with HIV infection. Although the CT appearance is not pathognomic, correlation results of aspiration cytology and with clinical history can lead to a preoperative diagnosis of a benign lymphoepithelial lesion.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

441

British Library Electronic Table of Contents (United Kingdom)

Glioblastoma multiforme is the most aggressive form of human brain tumor, which has no effective cure. Previously, we have demonstrated that overexpression of the C-terminal fragment of the human telomerase reverse transcriptase (hTERTC27) inhibits the growth and tumorigenicity of human cervical cancer HeLa cells. In this study, the therapeutic effect and molecular mechanisms of hTERTC27-mediated cancer gene therapy were further explored in vivo in established human glioblastoma xenografts in nude mice. We showed that intratumoral injection of adeno-associated virus carrying hTERTC27 (rAAV-hTERTC27) is highly effective in reducing the growth of the subcutaneously transplanted glioblastoma tumors. Histological analyses showed that rAAV-hTERTC27 treatment leads to profound necrosis, apoptosi...

2007-01-01

442

Computed Tomography of the diabetic kidney; La Tomografia Computerizzata nello studio del rene diabetico

Virtual colonoscopy (CT colonography) in the identification of colorectal cancer. A prospective study in symptomatic patients; Impiego dell'endoscopia virtuale con Tomografia Computerizzata nell'identificazione delle neoplasie colorettali. Studio prospettivo in pazienti sintomatici

The purpose of this work is to investigate whether any morphometric or densitometric are detectable in the kidneys of type 2 diabetic patients. 40 diabetic patients were examined and of 20 non-diabetics (the control group) were submitted to triphasic helical CT for different abdominal conditions. The type 2 diabetic patients were 23 men and 17 women, mean age 62 years, while the nondiabetic controls were 12 men and 8 women, mean age 58 years. All the CT images were analyzed using the Multiplanar Reconstruction (MPR) software. It was evaluated morphometric features, such as the presence of parenchymal or vascular calcifications, axial and coronal renal diameters, coronal renal area, and cortico medullary ratio, and densitometric and functional features, such as unenhanced renal density, cortical and medullary density in the arterial phase, parenchymal density in the nephrographic phase, and contrast elimination. It was also compared the results in the subgroups of patients with those in ...

2000-02-01

443

The CYP2A3 gene product catalyzes coumarin 7-hydroxylation in human liver microsomes

Aim of this study was to evaluate the sensitivity of virtual colonoscopy (CT colonography) in the identification of colorectal cancer and to define the limitations and the advantages of this imaging modality, as well as indications to the examination. It was examined prospectively 62 symptomatic patients aged 36 to 82 years (28 women and 34 men). All patients underwent both conventional and virtual colonoscopy on the same day; the conventional examination allowed exploration of the entire colon. Conventional colonoscopy identified 89 lesions 3-50 mm in diameter, namely 84 benign and 5 malignant lesions. No lesions were identified in 12 patients. CT colonography identified 52 of the 89 lesions, with 57.1% diagnostic accuracy. They were 11 false positives (82.5% positive predictive value and 52.2% specificity) and 37 false negatives (24.5% negative predictive value and 58.4% sensitivity). Sensitivity was significantly higher (85.7%) for polyps {>=} 1 cm. Virtual colonoscopy is an ...

2000-06-01

444

The CYP2A3 gene product catalyzes coumarin 7-hydroxylation in human liver microsomes

Three cDNAs, designated IIA3, IIA3v, and IIA4, coding for P450s in the CYP2A gene subfamily were isolated from a {lambda}gt11 library prepared from human hepatic mRNA. Only three nucleotide differences and a single amino acid difference, Leu{sup 160}{yields}His, were found between IIA3 and IIA3v, indicating that they are probably allelic variants. IIA4 displayed 94% amino acid similarity with IIA3 and IIA3v. The three cDNAs were inserted into vaccinia virus, and recombinant viruses were used to infect human hepatoma Hep G2 cells. Only IIA3 was able to produce an enzyme that had a reduced CO-bound spectrum with a {lambda}{sub max} at 450 nm. This expressed enzyme was able to carry out coumarin 7-hydroxylation and ethoxycoumarin O-deethylation. cDNA-expressed IIA3v and IIA4 failed to incorporate heme and were enzymatically inactive. Analysis of IIA proteins in human liver microsomes, using antibody against rat IIA2, revealed two proteins of 49 ...

1990-02-06

445

Susceptibility of the human retrovirus XMRV to antiretroviral inhibitors

Three cDNAs, designated IIA3, IIA3v, and IIA4, coding for P450s in the CYP2A gene subfamily were isolated from a #lambda#gt11 library prepared from human hepatic mRNA. Only three nucleotide differences and a single amino acid difference, Leu"1"6"0#->#His, were found between IIA3 and IIA3v, indicating that they are probably allelic variants. IIA4 displayed 94% amino acid similarity with IIA3 and IIA3v. The three cDNAs were inserted into vaccinia virus, and recombinant viruses were used to infect human hepatoma Hep G2 cells. Only IIA3 was able to produce an enzyme that had a reduced CO-bound spectrum with a #lambda#_m_a_x at 450 nm. This expressed enzyme was able to carry out coumarin 7-hydroxylation and ethoxycoumarin O-deethylation. cDNA-expressed IIA3v and IIA4 failed to incorporate heme and were enzymatically inactive. Analysis of IIA proteins in human liver microsomes, using antibody against rat IIA2, revealed two proteins of 49 and 50 ...

1990-02-01

446

Protein expression in white spot syndrome virus infected Penaeus monodon fabricius.

BackgroundXMRV (xenotropic murine leukemia virus-related virus) is the first known example of an exogenous gammaretrovirus that can infect humans. A limited number of reports suggest that XMRV is intrinsically resistant to many of the antiretroviral drugs used to treat HIV-1 infection, but is sensitive to a small subset of these inhibitors. In the present study, we used a novel marker transfer assay to directly compare the antiviral drug sensitivities of XMRV and HIV-1 under identical conditions in the same host cell type.ResultsWe extend the findings of previous studies by showing that, in addition to AZT and tenofovir, XMRV and HIV-1 are equally sensitive to AZddA (3'-azido-2',3'-dideoxyadenosine), AZddG (3'-azido-2',3'-dideoxyguanosine) and adefovir. These results indicate that specific 3'-azido or acyclic nucleoside analog inhibitors of HIV-1 reverse transcriptase (RT) also block XMRV infection with comparable efficacy in vitro. Our data ...

2010-08-31

447

Origin of XMRV and its Demise as a Human Pathogen Associated with Chronic Fatigue Syndrome.

White spot syndrome virus (WSSV) is the causative agent of the white spot disease of shrimp. Penaeus monodon were captured from Muttukadu Estuary in Chennai, India, transported to the laboratory and maintained in an aerated system with continuous water circulation-biofiltration. WSSV-free P. monodon were challenged by feeding them only once with WSSV-infected tissues of P. monodon. Cumulative mortality (100%) of the infected individuals was determined. Tissues from infected and uninfected shrimp such as muscles, hepatopancreas, heart, gills and eye tissues (100mg of each) and haemolymph (50 microl) were subjected to SDS-PAGE. In infected muscle tissue, six newly expressed proteins were detected. In infected haemolymph, four new proteins and three intensely expressed high molecular weight proteins were observed. Three intensely expressed high molecular weight proteins were detected in infected heart tissue and two new proteins in infected hepatopancreatic tissues. ...

2005-06-01

448

Friend Spleen Focus-Forming Virus Activates the Tyrosine Kinase sf-Stk and the Transcription Factor PU.1 to Cause a Multi-Stage Erythroleukemia in Mice.

Retroviruses are well known pathogens of mammals, birds and fish. Their potential to induce cancer in chickens was already described almost 100 years ago and murine retroviruses have been a subject of study for 50 years. The first human retroviruses, HTLV and HIV, were discovered more than 30 years ago, surprising researchers and physicians by the profound differences in the diseases they cause. HTLV-1 is able to induce, after decades of infection, lymphomas/leukemia or neuroimmune disorders whereas untreated HIV infection leads almost inevitably to AIDS. The recently described XMRV (xenotropic murine leukemia virus-related virus) appeared to possess many of the features known for HTLV and was regarded by some to be the third human retrovirus. However, recent publications by Knox et al. [1] and Paprotka et al. [2] have shed new light on this gammaretrovirus. Knox and colleagues clearly demonstrate that XMRV is absent in patients belonging to a ...

2011-07-27

449

Evaluation of the Performance of the Automated NucliSENS easyMAG and EasyQ Systems versus the Roche AmpliPrep-AMPLICOR Combination for High-Throughput Monitoring of Human Immunodeficiency Virus Load?

HEMATOLOGICAL MALIGNANCIES IN HUMANS TYPICALLY INVOLVE TWO TYPES OF GENETIC CHANGES: those that promote hematopoietic cell proliferation and survival (often the result of activation of tyrosine kinases) and those that impair hematopoietic cell differentiation (often the result of changes in transcription factors). The multi-stage erythroleukemia induced in mice by Friend spleen focus-forming virus (SFFV) is an excellent animal model for studying the molecular basis for both of these changes. Significant progress has been made in understanding the molecular basis for the multi-stage erythroleukemia induced by Friend SFFV. In the first stage of leukemia, the envelope protein encoded by SFFV interacts with and activates the erythropoietin (Epo) receptor and the receptor tyrosine kinase sf-Stk in erythroid cells, causing their Epo-independent proliferation, differentiation and survival. In the second stage, SFFV integration into the Sfpi1 locus activates the myeloid ...

2010-10-11

450

Effective use of optimized, high-dose (50 kGy) gamma irradiation for pathogen inactivation of human bone allografts.

This study presents the data of an evaluation of the automated Nuclisens easyMAG and EasyQ systems versus the Roche AmpliPrep-AMPLICOR combination for testing of high-volume human immunodeficiency virus (HIV) load. This represents a follow-up of a previous study investigating the performance of the real-time Nuclisens assay using the semiautomated NucliSENS miniMAG extraction procedure. Three hundred eighteen patient samples were analyzed using both methods. The easyMAG-EasyQ HIV type 1 system has a higher sensitivity and broader dynamic range than the Cobas AmpliPrep-AMPLICOR system when the standard Roche assay is used alone, 25 to 3,000,000 IU/ml versus 400 to 750,000 HIV RNA copies/ml, respectively. There was significant correlation between the assays (0.93; P < 0.0001), with good accuracy (percent similarity mean ? = 96%), good precision (percent similarity standard deviation = 4.97%), and overall good agreement with a low percent similarity coefficient of ...

2007-05-01

451

Development of recombinant adeno-associated virus and adenovirus cocktail system for efficient hTERTC27 polypeptide-mediated cancer gene therapy.

The safety of tissue allografts has come under increased scrutiny due to recent reports of allograft-associated bacterial and viral infections in tissue recipients. We report that 50 kGy of gamma irradiation, nearly three times the dose currently used, is an effective pathogen inactivation method when used under optimized conditions that minimize damage to the tissue. Cancellous bone dowels treated with a radioprotectant solution and 50 kGy of optimized irradiation had an ultimate compressive strength and modulus of elasticity equal to conventionally irradiated (18 kGy) and non-irradiated control bone grafts. We subjected bone dowels treated with this pathogen inactivation method to an in vitro cytotoxicity test using three different mammalian cell lines and concluded that the treated grafts were not cytotoxic. The log reduction of nine pathogens spiked into radioprotectant-treated bone irradiated to 50 kGy was also tested. We achieved 4.9 logs of inactivation of a model ...

2005-05-01

452

Depuration of shellfish by irradiation: Final technical report, October 1, 1987--March 31, 1989

The low in vivo transduction efficiency of recombinant adeno-associated virus (rAAV) and the undesirably strong immunogenicity of adenovirus (rAdv) have limited their clinical utilization in cancer gene therapy. We have previously demonstrated that intratumoral injection of rAAV expressing a C-terminal polypeptide of human telomerase reverse transcriptase (rAAV-hTERTC27) effectively inhibits the growth of glioblastoma xenografts in nude mice. To further improve its efficacy, we combined rAAV-hTERTC27 with rAdv and investigated the efficiency of the cocktail vectors in vivo. At a nontherapeutic dose (1 x 10(8) plaque-forming units (PFUs)), rAdv-null and rAdv-hTERTC27 were equipotent in enhancing the therapeutic efficacy of rAAV-hTERTC27 (1.5 x 10(11) v.g.), and complete tumor regression was achieved in 25% of the treated animals. Importantly, the combination of rAAV-hTERTC27 and a therapeutic dose (2.5 x 10(9) PFU) of rAdv-hTERTC27 significantly augmented the ...

2008-06-06

453

Characterization of hyaluronate binding proteins isolated from 3T3 and murine sarcoma virus transformed 3T3 cells

Studies by the University of Lowell Radiation Laboratory and the US National Marine Fisheries Service N.E. Laboratory in Gloucester, MA on softshelled clams (Mya arenaria) demonstrated the effectiveness of low to medium doses of Cobalt 60 source gamma irradiation in the inactivation of Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and Streptococcus faecalis. Post-irradiation survival and organoleptic studies when extended to hardshelled clams (Mercinaria mercenaria) and American oysters (Crassostrea virginica) showed no significant decline in consumer qualities or 6 day post irradiation survival in oysters at doses of up to 3.0 kGy. The capacities of the American oyster to sustain relatively high doses of gamma irradiation were demonstrated by 6 day post-exposure survivorship values of greater than 90% for samples receiving 3.0, 5.0 and 7.0 kGy. Initial studies of inactivation of Polio I virus and a simian rotavirus (SA-11) was conducted in both ...

454

Unusual Recharge Processes near Arroyos of the Rio Grande Aquifer, El Paso/Juarez Area

A hyaluronic acid binding fraction was purified from the supernatant media of both 3T3 and murine sarcoma virus (MSV) transformed 3T3 cultures by hyaluronate and immunoaffinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis resolved the hyaluronate affinity-purified fraction into three major protein bands of estimated molecular weight (M/sub r,e/) 70K, 66K, and 56K which contained hyaluronate binding activity and which were termed hyaluronate binding proteins (HABP). Hyaluronate affinity chromatography combined with immunoaffinity chromatography, using antibody directed against the larger HABP, allowed a 20-fold purification of HABP. Fractions isolated from 3T3 supernatant medium also contained additional binding molecules in the molecular weight range of 20K. This material was present in vanishingly small amounts and was not detected with a silver stain or with (/sup 35/S)methionine label. The three protein species isolated by ...

1987-06-02

455

Submarine tsunamigenic landslides at Stromboli Volcano: characterization and estimation of recurrence time

The twin-cities of El Paso and Juarez share the water resources of the Hueco Bolson aquifer and overlying Rio Grande aquifer. Both aquifers span the international border between Mexico and the United States. Salinity in the Rio Grande aquifer varies widely, some parts of the shallow aquifer containing less than 1,000 mg/L total dissolved solids (TDS), other parts of the aquifer exceeding 5,000 mg/L TDS. One sizable part of the "Lower Valley" area, approximately 45 km below El Paso contains very dilute water near the outer edge of the floodplain. Historically it had been thought that the dilute waters in this location were derived from recharge from arroyos that drained proximal parts of the Hueco Bolson. Instead, our hydrogen and oxygen isotope data and carbon-14 data indicate that these dilute waters were derived from pre-dam infiltration of the Rio Grande. Relatively light and slightly evaporated pre-dam waters (-11.5 del O18) at the arroyos are also ...

2005-12-01

456

Physicochemical characterization and thermal behavior of guanabana (Annona muricata) seed almond oil; Caracterizacion fisicoquimica y comportamiento termico del aceite de almendra de guanabana (Annona muricata, L)

Seafloor mapping and morphometric analysis of landslide scars can provide useful insights for marine geo-hazard assessment, as demonstrated by several studies performed on different geological settings. The availability of high-resolution multibeam bathymetry and long-range side scan sonar data on the submarine portions of Stromboli Volcano allow us to map and characterize the main mass-wasting features that affect, on the whole, about the 90% of its submarine extension. In particular, two main kinds of tsunamigenic landslides have been recognized and analyzed. Large-scale sector collapses (Fig. 1) are catastrophic events that mobilize 1-2 cubic kilometers of material, generating huge tsunami waves that may affect Stromboli and propagate in surrounding areas; related hazard is not very high, as they show recurrence periods of some (or more) thousand years. Conversely, medium-scale landslides are more hazardous, as they occur at higher frequency with respect to the previous events, i.e. ...

2010-12-01

457

Nuclear magnetic resonance spectroscopy in biomedicine. Course organized by the Istituto Superiore di Sanita`. Marciana Marina (Isola d`Elba), September 18-23, 1995; Spettroscopia di risonanza magnetica nucleare in biomedicina. Corso organizzato dall`Istituto Superiore di Sanita`. Marciana Marina (Isola d`Elba), 18-23 settembre 1995

In this work some physicochemical properties and the thermal behavior and stability of sour sop or guanabana (Annona muricata) seed almond oil were studied by means of chemical, DSC and TG analysis. The results showed that the almond has 2.5% ash, 17.9% crude fiber, 15.7% protein, 26.0% de carbohydrates and 37.7% oil (dry base). The composition of almond oil showed 68.5% unsaturated fatty acids, mainly oleic and linoleic, and some palmitoleic acids, and 31.5% saturated, principally palmitic and stearic fatty acids; refraction index was 1.468 and saponification and iodine value were 168.2 and 87.0, respectively. DSC thermal analysis showed that oil crystallization initiates at -4.5 degree centigrade and ends at -79.0 degree centigrade with a crystallization enthalpy of 48.2 J/g; the oil melts in a temperature range from -42.4 to -16.9 degree centigrade, with a maximum peak at -15 degree centigrade and a fusion enthalpy of 80.5 J/g. The oil remained liquid at refrigeration temperatures ...

2010-07-01

458

Machinery management data for willow harvest with a bio-baler

Nuclear magnetic resonance is a powerful technique that can be used in a wide range of applications, such as the structural characterization of high molecular weight molecules, conformational studies on enzymes in solution, enzyme-substrate or DNA-protein interactions, monitoring of cell metabolism in vivo, and for diagnostic purposes, employing spectroscopic and imaging techniques. This course was organized in order to introduce the participants to the fundamentals of NMR spectroscopy, and offer practical advice on performing NMR experiments on cell systems, cell and tissue extracts and animal models. The main implications regarding human experiments were also discussed. Finally the quantification of information and the interpretation of data were considered with regard to the main nuclei observed. [Italiano] La risonanza magnetica nucleare e` una delle tecniche spettroscopiche che meglio risponde all`ampio spettro di condizioni imposto dalla ricerca in biofisica e biomedicina. Il ...

1997-12-01

459

Increased Italian maritime transport alternative to road transport: Feasibility relative to normatives, technology, socio-economics and environment; Risparmio energetico ed uso razionale dell`energia nei trasporti: Aspetti normativi, tecnologici, socioeconomici ed ambientali del traffico merci nel cabotaggio

Willow harvested in 2-to-3-year rotations has been touted as a reliable source of biomass. A newly designed harvester based on a round baler was developed in 2006. The third generation biobaler was evaluated in 2009 at 2 willow plantations in Quebec. The first site at Godmanchester was a three-year old regrowth with an estimated 52,000 stems/ha of large diameter willow. The biobaler with a flail cutter harvested three plots totalling 4,136 m{sup 2}. Total harvested biomass was 10.36 t DM in 42 bales. The harvest rate averaged 29 bale/h with an average bale size of 1.22 m wide by 1.30 m in diameter. Diesel fuel consumption averaged 0.81 L/bale. Measured losses averaged 11 per cent of the yield. The second site at Saint-Roch-del'Achigan was a two-year old growth with an estimated 49,000 stems/ha of large stem willow. The biobaler harvested seven plots totalling 15,740 m{sup 2}. Total harvest was 30.70 t DM in 148 bales. The first 14 bales were harvested with ...

2010-07-01

460

Gene expression profiles deciphering rice phenotypic variation between Nipponbare (Japonica) and 93-11 (Indica) during oxidative stress.

This paper examines the technological, socio-economic and environmental feasibility of a proposal to strengthen the Italian commercial coastal shipping system as an alternative to bolstering existing road transport facilities in order to meet the requirements of increased trade in the new European free market. This prospect is of special importance to Italy due to its long coastlines. The principal elements taken into account are: investment capital, labour and energy requirements. The study first examines the supply and demand dynamics of the European transport system. Here, attention is given to energy consumption. This examination is followed by a review of the European situation with regard to normatives governing navigation and customs duties. Road, rail and maritime transport costs are compared, and an assessment is made of the economic feasibility of an integrated-means system of transport involving the coordinated use of special containers, tractor-trailers and interchangeable ...

1991-11-01

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

461

Evaluation of node involvement in non small cell bronchogenic carcinoma. CT-pathology correlation; Valutazione del fattore <<N>> nel carcinoma polmonare non a piccole cellule. Correlazione tra tomografia computerizzata e anatomia patologica

Rice is a very important food staple that feeds more than half the world's population. Two major Asian cultivated rice (Oryza sativa L.) subspecies, japonica and indica, show significant phenotypic variation in their stress responses. However, the molecular mechanisms underlying this phenotypic variation are still largely unknown. A common link among different stresses is that they produce an oxidative burst and result in an increase of reactive oxygen species (ROS). In this study, methyl viologen (MV) as a ROS agent was applied to investigate the rice oxidative stress response. We observed that 93-11 (indica) seedlings exhibited leaf senescence with severe lesions under MV treatment compared to Nipponbare (japonica). Whole-genome microarray experiments were conducted, and 1,062 probe sets were identified with gene expression level polymorphisms between the two rice cultivars in addition to differential expression under MV treatment, which were assigned as Core Intersectional Probesets ...

2010-01-08

462

Development and application of a third order scheme of finite differences centered in mesh; Desarrollo y aplicacion de un esquema de tercer orden de diferencias finitas centradas en malla

The main purpose of this work is to evaluate the role of CT in identifying other morphological signs of met static lymph node involvement from non small cell bronchogenic carcinoma. This is done to improve N staging, a critical step in this disease. In fact, since diameter is the only criterion used to distinguish normal form abnormal lymph nodes, mediastinal CT only has 80% diagnostic accuracy. 137 patients with known or suspected lung cancer were examined with Helical CT during early and late arterial phases (2 min. delay, 3 mm thickness, 5 mm inter slice gap) to depict node characteristics. Mediastinal lymph nodes, located according to the American Thoracic Society mapping, were considered normal when they were not visible or, if visible, less than 1 cm in diameter and of homogeneous density; lymph nodes over 1 cm in diameter and homogenous density were considered reactive. A lymph node was considered metastatic when, independent of size, the following signs were found: central hypo ...

2000-05-01

463

Defecographic diagnosis of the puborectalis syndrome; Diagnosi mediante defecografia della sindrome del muscolo puborettale

In this work the development of a third order scheme of finite differences centered in mesh is presented and it is applied in the numerical solution of those diffusion equations in multi groups in stationary state and X Y geometry. Originally this scheme was developed by Hennart and del Valle for the monoenergetic diffusion equation with a well-known source and they show that the one scheme is of third order when comparing the numerical solution with the analytical solution of a model problem using several mesh refinements and boundary conditions. The scheme by them developed it also introduces the application of numeric quadratures to evaluate the rigidity matrices and of mass that its appear when making use of the finite elements method of Galerkin. One of the used quadratures is the open quadrature of 4 points, no-standard, of Newton-Cotes to evaluate in approximate form the elements of the rigidity matrices. The other quadrature is that of 3 points of Radau ...

2003-07-01

464

CF findings of bowel infarction in 56 surgical patients, 5 of whom with reversible ischemia; Aspetti alla tomografia computerizzata dell'infarto intestinale. 56 pazienti sottoposti a interventi chirurgico di cui 5 con ischemia mesenterica reversibile.

The puborectalis syndrome is a defecation disorder supported by the nonrelaxation of puborectalis sling with consequent dyschezia. They report on a series of 98 patients submitted to clinical examination, defecography, anorectal manometry, electromyography and intestinal transit time studies. The main symptoms of puborectalis syndrome in their patients were incomplete defecation (89 %) and intermittent evacuation (63 %); 28 % of patients turned to finger defecation. In all patients, defecography showed an abnormal increase in puborectalis impression on the posterior anorectal wall, reduced anorectal angle opening under straining (mean value: 113 deg) and prolonged expulsion time with barium pooling in the ampulla (mean evacuation time: 38 seconds). Such anorectal abnormalities as rectal mucosal prolapse (47 cases) and anterior rectocele (36 cases) were also associated. In 33 of 98 patients (34 % of cases), sling assessment by bidigital palpation at preliminary clinical examination ...

1997-04-01

465

19. National seminar on the rational use of energy and exposition of equipment and services. Transactions. [Selected Papers]; 19. Seminario nacional sobre el uso racional de la energia y exposicion de equipos y servicios. Memoria Tecnica. [Ponencias seleccionadas

Bowel infarction is a rare and typical condition of the elderly; despite improvements in diagnostic imaging and vascular surgery, bowel infarction remains a major cause of acute abdomen, with mortality rates ranging 70-80%. Diagnosis on often late because clinical signs, laboratory data and radiologic findings are a specific. The authors investigated radiographic and particular CT patterns of intestinal infraction in 56 patients submitted to surgery within 12 hours of admission. We also report the CT findings of 5 of these patients who had reversible mesenteric ischemia. CT is more sensitive than radiography and does not exhibit the limitations of angiography (i.e., invasiveness, radiation exposure and complex organization). Therefore CT can presently be considered the method of choice in patients with suspected bowel infarction. [Italian] L'infarto intestinale rappresenta una malattia rara e tipica del soggeto anziano; nonostante il notevole ...

1999-04-01

466

Towards Quantum Superposition of Living Organisms

This document contains the technical proceedings of the 19 national seminar on the rational use of energy and exposition of equipment and services, organized by the Asociacion de Tecnicos y Pofesionistas en Aplicacion Energetica (ATPAE) (Association of Technicians and Professionals on Energy Application) conducted in Mexico City on September 21-25, 1998. This seminar encompassed subjects such as: energy saving in the energy sector, energy efficiency in industrial processes and practical developments, practical cases of energy saving in industry, energy saving in buildings, transportation, non-renewable energy utilization and environmental impact and eco-energies. [Espanol] Este documento contiene la memoria tecnica del 19 seminario nacional sobre el uso racional de la energia y exposicion de equipos y servicios, organizado por la Asociacion de Tecnicos y Profesionistas en Aplicacion Energetica (ATPAE), en Mexico, D.F, los dias 21-25 de septiembre de 1998. Este ...

1998-12-31

467

Shellfish depuration by gamma irradiation: Final report for the period October 1, 1985-September 30, 1986

The most striking feature of quantum mechanics is the existence of superposition states, where an object appears to be in different situations at the same time. Up to now, the existence of such states has been tested with small objects, like atoms, ions, electrons and photons, and even with molecules. Recently, it has been even possible to create superpositions of collections of photons, atoms, or Cooper pairs. Current progress in optomechanical systems may soon allow us to create superpositions of even larger objects, like micro-sized mirrors or cantilevers, and thus to test quantum mechanical phenomena at larger scales. Here we propose a method to cool down and create quantum superpositions of the motion of sub-wavelength, arbitrarily shaped dielectric objects trapped inside a high--finesse cavity at a very low pressure. Our method is ideally suited for the smallest living organisms, such as viruses, which survive under low vacuum pressures, and optically behave ...

2009-01-01

468

Regulation of human ribosomal RNA transcription.

Irradiation of live bivalve molluscs is a promising new technique to reduce the public health threats of viral and bacterial pathogens in the consumption of raw shellfish. In studies conducted within the past year, live hardshelled clams and oysters were innoculated with Simian Rotavirus SA-11, Poliovirus I, and Hepatitis-A virus and treated with gamma ray ionizing radiation from University of Lowell/DOE 800,000 Curie "6"0Co source over a dose range of 0.5--10 kiloGray (50--1000 kilorad) to determine the effectiveness of the process in eliminating viral activity and thus easing the potential viral health threats associated with uncooked bivalves. Two viral strains, SA-11 and Poliovirus I, were employed as models to perfect laboratory technique and to estimate the most appropriate range of dose exposures necessary for optimal inactivation of the infectious pathogen, Hepatitis A. Post-irradiation survivorship of the oysters and quahogs was assessed over a range of ...

469

Rearrangement of a common cellular DNA domain on chromosome 4 in human primary liver tumors

We have used a cell-free polymerase I transcription system derived from HeLa cells to study the regulation of human rRNA synthesis. Analysis of deletion mutants spanning the start site of transcription at nucleotide +1 indicates that the control region affecting initiation of human rRNA synthesis is contained within sequences from nucleotides -158 to +18. This promoter region can be subdivided into (i) a central segment of approximately 40 base pair that is required for transcription and (ii) flanking sequences that influence the efficiency of transcription in vitro. We have examined the in vitro transcriptional activity of the human extract under various conditions that are thought to modulate rRNA synthesis in vivo. Cell-free extracts prepared from HeLa cells infected with adenovirus 2 synthesize human rRNA at levels greatly decreased relative to uninfected cell extracts. By contrast, in vitro transcription of human rRNA is stimulated 2- to 3-fold by the addition of purified simian ...

1983-06-01

470

Rearrangement of a common cellular DNA domain on chromosome 4 in human primary liver tumors

Hepatitis B virus (HBV) DNA integration has been shown to occur frequently in human hepatocellular carcinomas. The authors have investigated whether common cellular DNA domains might be rearranged, possibly by HBV integration, in human primary liver tumors. Unique cellular DNA sequences adjacent to an HBV integration site were isolated from a patient with hepatitis B surface antigen-positive hepatocellular carcinoma. These probes detected rearrangement of this cellular region of chromosomal DNA in 3 of 50 additional primary liver tumors studied. Of these three tumor samples, two contained HBV DNA, without an apparent link between the viral DNA and the rearranged allele; HBV DNA sequences were not detected in the third tumor sample. By use of a panel of somatic cell hybrids, these unique cellular DNA sequences were shown to be located on chromosome 4. Therefore, this region of chromosomal DNA might be implicated in the formation of different tumors at one step of ...

1988-02-01

471

Radiosynthesis of hydrogel confined to hollow-fiber membranes for the design of a bioartificial extra-corporeal liver

Hepatitis B virus (HBV) DNA integration has been shown to occur frequently in human hepatocellular carcinomas. The authors have investigated whether common cellular DNA domains might be rearranged, possibly by HBV integration, in human primary liver tumors. Unique cellular DNA sequences adjacent to an HBV integration site were isolated from a patient with hepatitis B surface antigen-positive hepatocellular carcinoma. These probes detected rearrangement of this cellular region of chromosomal DNA in 3 of 50 additional primary liver tumors studied. Of these three tumor samples, two contained HBV DNA, without an apparent link between the viral DNA and the rearranged allele; HBV DNA sequences were not detected in the third tumor sample. By use of a panel of somatic cell hybrids, these unique cellular DNA sequences were shown to be located on chromosome 4. Therefore, this region of chromosomal DNA might be implicated in the formation of different tumors at one step of ...

472

Molecular resemblance of an AIDS-associated lymphoma and endemic Burkitt lymphomas: Implications for their pathogenesis

Current bioartificial extra-corporeal systems are bioreactors where cells are separated from the surrounding media by porous polymeric membranes. The present work focuses on the design of membranes that allow the differential diffusion of plasma metabolites and proteins such as immunoglobulin (IgG). This design will improve catabolites removal and reduce possible immune response and virus infection. We demonstrate the feasibility to synthesize the hydrogels confined to the macroporous structure of membranes by radiation-induced in situ polymerization. The hollow-fiber membranes were soaked in aqueous monomeric solution, rinsed and irradiated while submerged in oil. This procedure confined the hydrogel to the void internal volume of the pores of the membrane. Hydrogels of polyacrylamide and polyHEMA were synthesized this way by irradiation at 10 kGy. Hydraulic permeability and diffusion of glucose, albumin and IgG were measured in these hydrogel-filled membranes. ...

2007-08-15

473

MR findings of central nervous system involvement in acquired immunodeficiency syndrome patient : a report of two cases

Non-Hodgkin lymphoma is a common feature of AIDS. Approximately 30-40% of these tumors exhibit clinical features suggestive of endemic Burkitt lymphoma: they are aggressive malignancies that occur in association with Epstein-Barr virus infection, they arise in the setting of immunosuppression, and they carry t(8;14) translocations without detectable rearrangement of the MYC oncogene. To understand the molecular basis of these parallels, the authors analyzed a case of Epstein-Barr-positive AIDS-associated undifferentiated lymphoma. Southern blots show that the tumor exhibits immunoglobulin joining segment rearrangement but no rearrangement of the MYC oncogene. Cloning of the rearranged joining segment allowed the isolation of recombinant clones encompassing the translocation breakpoint, and sequencing of the translocation junction disclosed that the breakpoint is situated 7 base pairs from the chromosome 14 site involved in a previously described endemic Burkitt ...

474

Increased transcription of the c-myc oncogene in two methylcholanthrene-induced quail fibroblastic cell lines

Central nervous system (CNS) manifestations in acquired immunodeficiency syndrome (AIDS) patients are an early and common feature. The spectrum of AIDS-related CNS diseases are encephalitis caused by the human immunodeficiency virus(HIV) itself, opportunistic infection, infarct and malignancy. We experienced two cases of CNS involvement in AIDS and they were serologically diagnosed as HIV encephalitis and CNS toxoplasmosis, respectively. In the case of the HIV encephalitis patient, brain MRI showed a non-enhancing lesion with high signal intensity on T2WI and low signal on T1WI and there was no mass effect on the right frontal lobe, periventricular white matter, splenium of the corpus callosum or bilateral basal ganglia. In the other case of CNS toxoplasmosis, MR showed multiple nodular and rim enhanced mass lesions in the right basal ganglia, thalamus and periventricular white matter, which were of low signal intensity on T1WI and of high intensity on T2WI. We ...

1996-10-01

475

Conocimiento de Transmision de SIDA y Percepcion Hacia los Ninos con SIDA en el Salon de Clases de los Maestros de Educacion Especial (Knowledge of AIDS Transmission and Special Education Teachers' Attitudes towards Children with AIDS in the Classroom).

The expression of three c-onc genes (c-erb, c-myc, c-myb) was investigated in five cell lines established from fibrosarcomas induced with 20-methylcholanthrene (MCA) of Japanese quails. These cell lines showed low levels of the three c-onc genes, with the exception of two cell lines that accumulated moderate (MCAQ 1-4) and large amounts (MCAQ 3-5) of c-myc RNA. Molecular cloning and restriction endonuclease analyses indicated that expression of c-myc in these two cell lines were not associated with detectable rearrangements in the c-myc locus, that the size of the c-myc transcript (2.7 kb) in MCAQ 3-5 was similar to that of the normal c-myc messenger RNAs (mRNA) and that the transcriptional activatin observed in MCAQ 3-5 was not mediated by the LTR (long terminal repeat) of a proximate ALV (avian leukosis virus) provirus. Finally, when analyzed with the restriction enzymes Msp I and Hpa II, the c-myc locus of MCAQ 3-5 and MCAQ 1-4 was found hypomethylated as ...

1984-12-01

476

Computer Network Security: Best Practices for Alberta School Jurisdictions.

This Spanish-language master's thesis presents a study which measured special education teachers' knowledge of AIDS (Acquired Immune Deficiency Syndrome) virus transmission and their attitudes toward children with AIDS in schools. Attitudes were then related to social variables such as sex, teacher's age, and knowing someone with AIDS. A survey of 101 Oswego County, New York, special education teachers found that 52 percent had a moderate knowledge about AIDS transmission, and 48 percent of respondents had a positive perception of children with AIDS. Findings also indicated that teachers had received effective training and information about AIDS; teachers seemed to feel insecure about applying that knowledge to particular situations of possible risk; knowing people with AIDS provoked negative or inadequate perceptions of that population; and age, sex, and teaching experience were not related to teachers' perceptions or AIDS knowledge. Several recommendations are ...

1993-12-01

477

Bayesian Based Comment Spam Defending Tool

This paper provides a snapshot of the computer network security industry and addresses specific issues related to network security in public education. The following topics are covered: (1) security policy, including reasons for establishing a policy, risk assessment, areas to consider, audit tools; (2) workstations, including physical security, protecting workstation components, and computer viruses; (3) the local network, including the OSI (Open Systems Interconnection) reference model, protocols, network segmentation, network management, network sniffing, and data encryption; (4) servers, including UNIX and other server operating systems; (5) remote access, including technologies, remote access servers, protocols, and authentication/authorization; (6) crackers and hackers, including threats and hacking tools/techniques; (7) Internet firewalls, including functions, issues and problems, types, rules, logs, firewall accessories, buying a firewall, and firewall ...

1999-02-01

478

Argentinian doctors accused of spreading AIDS.

Spam messes up user's inbox, consumes network resources and spread worms and viruses. Spam is flooding of unsolicited, unwanted e mail. Spam in blogs is called blog spam or comment spam.It is done by posting comments or flooding spams to the services such as blogs, forums,news,email archives and guestbooks. Blog spams generally appears on guestbooks or comment pages where spammers fill a comment box with spam words. In addition to wasting user's time with unwanted comments, spam also consumes a lot of bandwidth. In this paper, we propose a software tool to prevent such blog spams by using Bayesian Algorithm based technique. It is derived from Bayes' Theorem. It gives an output which has a probability that any comment is spam, given that it has certain words in it. With using our past entries and a comment entry, this value is obtained and compared with a threshold value to find if it exceeds the threshold value or not. By using this concept, we developed a software ...

2010-01-01

479

A single amino acid substitution results in a retinoblastoma protein defective in phosphorylation and oncoprotein binding

The provincial ministry of health has taken over a dialysis center in La Plata, Argentina, following the discovery that at least 20 of the clinic's 34 regular patients have been infected with the human immunodeficiency virus (HIV). Catheters of the clinic's dialysis machines were found to be contaminated with HIV. The 5 physicians associated with the Centro Modelo de Dialisis have been discharged for negligence. 20 of the regular patients also contracted hepatitis C. Argentinian law stipulates that filters for the same patient must be changed after 4 times and prohibits the use of the same vessel to administer medication to more than 1 patient. Testimony from nurses at the La Plata clinic revealed that filters were reused for several patients and basic sanitary procedures were not followed. In 1990, 33 kidney disease patients at a dialysis center in Cordoba became infected with HIV and it was discovered that filters had been used up to 8 times for different ...

1993-09-01

480

In vitro evaluation of herpes simplex virus type 1 thymidine kinase reporter system in dynamic studies of transcriptional gene regulation

The authors have previously identified a small-cell lung cancer cell line (NCI-H209) that expresses an aberrant, underphosphorylated form of the retinoblastoma protein RB1. Molecular analysis of RB1 mRNA from this cell line revealed a single point mutation within exon 21 that resulted in a nonconservative amino acid substitution (cysteine to phenylalanine) at codon 706. Stable expression of this mutant RB1 cDNA in a human cell line lacking endogenous RB1 demonstrated that this amino acid change was sufficient to inhibit phosphorylation. In addition, this cysteine-to-phenylalanine substitution also resulted in loss of RB1 binding to the simian virus 40 large tumor and adenovirus E1A transforming proteins. These results confirm the importance of exon 21 coding sequences and suggest that the cysteine residue at codon 706 may play a role in achieving a specific protein conformation essential for protein-protein interactions.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

481

Organizational models for new economy in the public and the private one; Modelli organizzativi per la new economy nel pubblico e nel privato

The herpes simplex virus type 1 thymidine kinase (HSV1-TK) reporter system is being used to directly and indirectly monitor therapeutic gene expression, immune cell trafficking and protein-protein interactions in various living animals. However, the issues of HSV1-TK enzyme stability in living cells and whether this reporter system is optimal for dynamic studies of gene expression events in genetic imaging have not be addressed. The purpose of the present study was to evaluate the application of this reporter system in dynamic studies of transcriptional gene regulation. To achieve this purpose, we established two tetracycline-inducible murine sarcoma cell lines, tetracycline-turn-off HSV1-tk-expressing cell line (NG4TL4/tet-off-HSV1-tk) and tetracycline-turn-off Luc-expressing cell line (NG4TL4/tet-off-Luc), to create an artificially regulated gene expression model in vitro. The dynamic transcriptional events mediating a series of doxycycline (Dox) inductions were ...

2006-07-15

482

Importance of the planning activities, cutting, handling and analysis of the nuclei of oil well drilling; Importancia de las actividades de planificacion, corte, manejo y analisis de los nucleos de perforacion de pozos petroleros

Historically the organizational models have followed an evolution that has underlined a parallel trend to the complexity of the market, introducing as different mechanisms of coordination. Up to yesterday the winning model it was that hierarchical that however it is not certainly immune from limits. Beginning from eight hundred and especially in the nine hundred experts of organization have studied an alternative or supplementary model to that hierarchical: the bureaucratic model. This last entrusts the organization of the firm to the norms and the procedures considering them like the best integrating mechanism of development of the organizations. Today it is not made that to speak of the model to net, but it would be a serious error if this had to a substitutive model thought of the precedents, that can work then without hierarchy and without alone procedures entirely thanks to the use of Internet. In the present job are individualized new models of management of the private firms and ...

2001-07-01

483

Nephron-sparing percutaneous ablation of a 5 cm renal cell carcinoma by superselective embolization and percutaneous RF-ablation

In the geologic formation where the oil deposits are located, diverse types of rocks perform functions as structural elements, storage containers for hydrocarbon water and calorific energy, as well as impermeable barriers and transport means of the energy and the fluids. Among the most important properties that are used to describe these functions are the porosity, the permeability, the compressibility of the pores volume, the formation resistivity factor, the saturation exponent, the velocity of acoustic waves P and S, the relative permeability, the capillary pressures, the elastic constants and other mechanical properties, the thermal expansion, coefficient, the thermal conductivity, the thermal diffusivity and the specific heat. The execution of an ample variety of activities related to the stages of exploration, location, evaluation and development of the oil deposits, depends strongly on having a good knowledge of the magnitude and the spatial variability of these properties. The ...

2007-07-01

484

Banks, environment and sustainable development. EMAS regulation n. 761/2001/EC applied to the financial institutions; Banche, ambiente e sviluppo sostenibile l'adesione degli istituti finanziari al regolamento EMAS

Purpose: To report on the nephron-sparing, percutaneous ablation of a large renal cell carcinoma by combined superselective embolization and percutaneous radiofrequency ablation. Materials and Methods: A 5 cm renal cell carcinoma of a 43-year-old drug abusing male with serologically proven HIV, hepatitis B and C infection, who refused surgery, was superselectively embolized using microspheres (size: 500 - 700 {mu}m) and a platinum coil under local anesthesia. Percutaneous radiofrequency ablation using a 7F LeVeen probe (size of expanded probe tip: 40 mm) and a 200 Watt generator was performed one day after transcatheter embolization under general anesthesia. Results: The combined treatment resulted in complete destruction of the tumor without relevant damage of the surrounding healthy renal tissue. The patient was discharged 24 hours after RF ablation. No complications like urinary leaks or fistulas were observed and follow up CT one day and 4 weeks after the radiofrequency ...

2001-11-01

485

Silica, silicosis and tuberculosis.

The purpose of this work is to characterize the role of the financial institutions in achieving sustainable development meaning a process of development which leaves at least the same amount of capital, natural and man-made, to future generations as current generations have access to. This makes it clear that sustainable development is about capital allocation and thus should be at the core of financial markets activity. On a more practical level the different ways banks affect environment are analyzed: as investors - supplying the investments with environmental perspective; as lenders - developing new financial products to encourage sustainable development; as valuers - estimating risks and opportunities in project-financing; as powerful stake holders - as shareholders and lenders they can exercise considerable influence over companies; - as polluters - while not dirty industries, financial institutions do consume natural resources. By the study of these environmental aspects it comes ...

2001-07-01

486

Risk of malignant lymphoma in patients with inflammatory bowel diseases: A Dutch nationwide study.

Exposure to crystalline silica dust causes multiple diseases, but silicosis and silica dust-associated tuberculosis (TB), in particular, are the two diseases that remain high on the list of occupational health priorities in low-income countries and that still occur in some high-income countries. The prevalence of silica-related TB is exacerbated by the human immunodeficiency virus (HIV) epidemic in low-income countries. This review describes the morphology of silica and the variable potency of the different forms. Sources of crystalline silica are discussed, with emphasis on less commonly recognised sources, such as small-scale mining operations and agriculture. Trends in the prevalence of silicosis are also presented. Although efforts have been made for many years in most countries to reduce silica dust levels, silicosis continues to occur even in young people. The clinical and pathological features and diagnosis of silicosis, with emphasis on chest radiography, ...

2007-05-01

487

Relationship of DNA repair processes to mutagenesis and carcinogenesis in mammalian cells. Progress report, November 1, 1979-October 31, 1980

BACKGROUND: Immune suppressant medications such as thiopurines and anti-tumor necrosis factor agents are important for maintaining disease control in most patients with inflammatory bowel diseases (IBDs); however, their use has been associated with the development of malignant lymphoma. The purpose of this Dutch nationwide study was to estimate the relative risk of malignant lymphoma in IBD patients. METHODS: IBD patients who developed a lymphoma between 1997 and 2004 were identified using the Dutch National Database of PALGA. Data from confirmed cases were collected from individual hospitals, including data on Epstein-Barr virus (EBV). The age-adjusted 8-year incidence of malignant lymphoma in the Netherlands was retrieved from the Central Bureau of Statistics. RESULTS: Forty-two hospitals were visited and 285 matches evaluated in the total cohort of 17,834 IBD patients. Forty-four lymphomas were observed, resulting in a relative risk of 1.27 (95% confidence ...

2010-12-22

488

Regional inventory of environmental health: experience in Southeastern France; Tableau de bord regional sante-environnement en Provence-Alpes-Cotes d'Azur: retour d'experience

The objective of this research is to determine the role of DNA repair in mutagenesis and carcinogenesis in mammalian cells. Use of the host-cell reactivation viral suicide enrichment procedure was initiated in the isolation of repair-deficient mutants. Lightly mutagenized BHK cells were infected with irradiated Herpes simplex virus (HSV); several radiation-sensitive strains were isolated among the survivors of the infection. The characterization of these strains is progressing and the enrichments are continuing. That alterations in the frequency of mutation of C3H/10T 1/2 cells, occurring as a result of holding the cells in a confluent state following treatment with ethylmethane sulfonate, parallel the alterations in the frequency of neoplastic transformation was found. The repair capabilities of BHK cells were found to be intermediate in comparison to repair-proficient and -deficient human cells with regard to the reactivation of HSV treated with various ...

489

Prospective benefit and effect of lipiodol marking in hepatocellular carcinoma; Moeglicher Nutzen und Auswirkung einer Lipiodolmarkierung auf die Histologie beim hepatozellulaeren Karzinom

In 2004, the Southeastern France regional council asked the regional health observatory to conduct an environmental health inventory to i) identify environmental health problems in this area; ii) examine the relations between environmental exposure to pollutants and human health; and iii) help public officials to identify priorities in this area. We collected and validated data from national, regional and local institutions, constructed environmental (levels of emissions and pollutants), health (mortality, incidence, prevalence) and behavioral indicators (e.g., complaints about odors), and compared these over time and between places when possible. For each topic, we summarized current knowledge about the links between environment and health. In southeastern France, as in other French areas, indoor air pollution, home and leisure injuries and other home-related risks are public health issues. Other topics are more significant or particular to southeastern France: transportation (air ...

2005-07-15

490

Potent, selective and cell penetrant inhibitors of SF-1 by functional ultra-high-throughput screening.

Purpose: To assess the benefit and the effect of intraarterial lipiodol application on histological analysis of patients suspected of having HCC. To determine whether lipiodol marking leads to a better biopsy result and how lipiodol or a transarterial chemoembolization influences the histological diagnosis. Materials and Methods: Two groups of patients were examined. Group A included 14 patients highly suspected of having an HCC which had previously been unsuccessfully biopsied. A transarterial embolization with lipiodol was performed in these patients to mark intrahepatic tumors which could not otherwise be defined in unenhanced CT. A biopsy was then repeated. Group B included 22 patients undergoing therapy using transarterial chemoembolization (TACE). A biopsy was performed to support the diagnosis of a multifocal HCC before changing the therapy. Results: The transversal diameters of the biopsied tumors were similar in both groups (A: 22 mm; B: 21 mm). The length of percutaneous ...

2005-10-01

491

One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase.

The steroidogenic factor 1 (SF-1, also known as NR5A1) is a transcription factor belonging to the nuclear receptor superfamily. Whereas most of the members of this family have been extensively characterized, the therapeutic potential and pharmacology of SF-1 still remains elusive. Described here is the identification and characterization of selective inhibitory chemical probes of SF-1 by a rational ultra-high-throughput screening (uHTS) strategy. A set of 64,908 compounds from the National Institute of Health's Molecular Libraries Small Molecule Repository was screened in a transactivation cell-based assay employing a chimeric SF-1 construct. Two analogous isoquinolinones, ethyl 2-[2-[2-(2,3-dihydro-1,4-benzodioxin-7-ylamino)-2-oxoethyl]-1-oxoisoquinolin-5-yl]oxypropanoate (SID7969543) and ethyl 2-[2-[2-(1,3-benzodioxol-5-ylmethylamino)-2-oxoethyl]-1-oxoisoquinolin-5-yl]oxypropanoate and (SID7970631), were identified as potent submicromolar inhibitors, yielding IC(50) values of 760 and ...

2008-03-11

492

Host resistance to rat cytomegalovirus (RCMV) and immune function in adult PVG rats fed herring from the contaminated Baltic Sea

We describe the cloning and characterization of a mutated thermostable DNA polymerase from Thermus aquaticus (Taq) that exhibits an increased reverse transcriptase activity and is therefore designated for one-step PCR pathogen detection using established real-time detection methods. We demonstrate that this Taq polymerase mutant (Taq M1) has similar PCR sensitivity and nuclease activity as the respective Taq wild-type DNA polymerase. In addition, and in marked contrast to the wild-type, Taq M1 exhibits a significantly increased reverse transcriptase activity especially at high temperatures (>60 degrees C). RNA generally hosts highly stable secondary structure motifs, such as hairpins and G-quadruplexes, which complicate, or in the worst case obviate, reverse transcription (RT). Thus, RT at high temperatures is desired to weaken or melt secondary structure motifs. To demonstrate the ability of Taq M1 for RNA detection of pathogens, we performed TaqMan probe-based diagnostics of ...

2010-02-01

493

Host based internet protocol (IP) packet analysis to enhance network security

We carried out a feeding study in rats aimed at extending our aboservations of contaminant-induced immunosuppression in harbour seals. Two herring batches were freeze-dried, supplemented and fed to female adult PVG rats for a period of 4 1/2 months. Daily contaminant intakes of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxic equivalents (TEQ) were estimated to be 0.3 ng/kg body weight and 1.6 ng/kg in the Atlantic and Baltic groups, respectively. At the end of the feeding experiment, no contaminant-related changes in spleen CD{sub 4}{sup +}/CD{sub 8}{sup +} cellularity, natural killer cell activity, or mitogen-induced proliferative responses of thymus or spleen cells could be detected. However, total thymocyte numbers and thymus CD{sub 4}{sup +}/CD{sub 8}{sup +} ratios were reduced in the Baltic group. A novel model was established to assess the specific T-cell response to rat cytomegalovirus (RCMV). When applied to the feeding study, no differences between the Atlantic and Baltic ...

1996-08-01

494

Genomic analysis of the symbiotic marine crenarchaeon, Cenarchaeumsymbiosum

Data communication in a computer network environment is facing serious security threats from numerous sources such as viruses, worms, Zombies etc. These threats can be broadly characterized as internal or external security threats. Internal threats are mainly attributed to sneaker-nets, utility modems and unauthorized users, which can be minimized by skillful network administration, password management and optimum usage policy definition. The external threats need more serious attention as these attacks are mostly coming from public networks such as Internet. Frequency and complexity of such attacks is much higher as compared to internal attacks. This paper presents a host based network layer screening of external and internal IP packets for logging, analyzing and real-time detection of possible IP spoofing and Denial of Service attacks. This work can also be used in tuning security rules definition for gateway firewalls. Software has been developed which ...

2007-01-01

495

Genetic control of immune response to pseudorabies and atrophic rhinitis vaccines: II. Comparison of additive direct and maternal genetic effects.

Crenarchaea are ubiquitous and abundant microbial constituents of soils, sediments, lakes and ocean waters, yet relatively little is known about their fundamental evolutionary, ecological, and physiological properties. To better describe the ubiquitous nonthermophilic Crenarchaea, we analyzed the genome sequence of one representative, the uncultivated sponge symbiont, Cenarchaeum symbiosum. C. symbiosum genotypes coinhabiting the same host partitioned into two dominant populations, corresponding to previously described a- and b-type ribosomal RNA variants. Although synthetic, overlapping a- and b-type ribotypes harbored significant genetic variability. A single tiling path comprising the dominant a-type genotype was assembled, and used to explore the biological properties of C. symbiosum and its planktonic relatives. Out of a total of 2,066 predicted open reading frames, 36% were more highly conserved with other Archaea. The remainder partitioned between bacteria (18%), eukaryotes ...

2006-06-24

496

Crystal and solution structures of an odorant-binding protein from the southern house mosquito complexed with an oviposition pheromone

Data from 52 litters farrowed in two seasons of a cross-fostering experiment were analyzed to estimate variances and covariances for additive direct and maternal genetic effects on immune response to pseudorabies virus and B. bronchiseptica vaccine. Twenty purebred boars and 44 sows of the Duroc, Landrace and Yorkshire breeds were used. Immune response was measured after vaccine challenge. A modified-live pseudorabies (PR) vaccine was administered to piglets at 28 d of age; response was measured by log2 serum neutralization titers at 56 d. An inactivated B. bronchiseptica bacterin was administered at 28, 42 and 112 d. Antibody levels were measured relative to positive and negative controls at 28, 56 and 119 d by using an enzyme-linked immunosorbent assay (ELISA). Results from this study for heritability were .18 +/- .09 for PR titer and .15 +/- .07 and .52 +/- .15 for 56- and 119-d ELISA values, respectively. The variability due to nurse environment (maternal ...

1987-02-01

497

Biomarkers of fibrosis and impaired liver function in chronic hepatitis C: how well do they predict clinical outcomes?

Culex mosquitoes introduce the pathogens responsible for filariasis, West Nile virus, St. Louis encephalitis, and other diseases into humans. Currently, traps baited with oviposition semiochemicals play an important role in detection efforts and could provide an environmentally friendly approach to controlling their populations. The odorant binding proteins (OBPs) in the female's antenna play a crucial, if yet imperfectly understood, role in sensing oviposition cues. Here, we report the X-ray crystallography and NMR 3D structures of OBP1 for Culex quinquefasciatus (CquiOBP1) bound to an oviposition pheromone (5R,6S)-6-acetoxy-5-hexadecanolide (MOP). In both studies, CquiOBP1 had the same overall six-helix structure seen in other insect OBPs, but a detailed analysis revealed an important previously undescribed feature. There are two models for OBP-mediated signal transduction: (i) direct release of the pheromone from an internal binding pocket in a ...

2010-11-15

498

Antitumor and Antiviral Activity of Colombian Medicinal Plant Extracts

PURPOSE OF REVIEW: To review the recent literature on the prognostic value of biomarkers of liver fibrosis and impaired liver function in patients with chronic hepatitis C with or without HIV coinfection. RECENT FINDINGS: A combination of standard blood tests seems to be useful in identifying patients at risk of liver-related complications. Findings from studies investigating the validity of the Model for End-Stage Liver Disease (MELD) score in HIV-infected liver transplant candidates are conflicting. Two large studies of HIV/hepatitis C virus (HCV) coinfected patients have shown that plasma levels of the fibrosis marker hyaluronic acid are a strong predictor of clinical complications. A smaller study found hyaluronic acid and two other fibrosis tests, aspartate aminotransferase-to-platelet ratio index (APRI) and Fib-4, to be independent predictors of mortality when included in models with the MELD or the Child-Pugh-Turcotte scores. SUMMARY: Although the data are ...

2010-01-01

499

Scientific Electronic Library Online (English)

Abstract in english Extracts of nine species of plants traditionally used in Colombia for the treatment of a variety of diseases were tested in vitro for their potential antitumor (cytotoxicity) and antiherpetic activity. MTT (Tetrazolium blue) and Neutral Red colorimetric assays were used to evaluate the reduction of viability of cell cultures in presence and absence of the extracts. MTT was also used to evaluate the effects of the extracts on the lytic activity of herpes simplex virus type (more) 2 (HSV-2). The 50% cytotoxic concentration (CC50) and the 50% inhibitory concentration of the viral effect (EC50) for each extract were calculated by linear regression analysis. Extracts from Annona muricata, A. cherimolia and Rollinia membranacea, known for their cytotoxicity were used as positive controls. Likewise, acyclovir and heparin were used as positive controls of antiherpetic activity. Methanolic extract from Annona sp. on HEp-2 cells presented a CC50 value at ...

1999-07-01

500

A single amino acid substitution (R441A) in the receptor-binding domain of SARS coronavirus spike protein disrupts the antigenic structure and binding activity