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Physiological and physico-chemical characterization of dietary fibre from the green seaweed Ulva fasciata Delile Caracterização fisiológica e físico-química da fibra alimentar da macroalga marinha verde Ulva fasciata Delile  

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This work aims to assess the potential of the green seaweed Ulva fasciata Delile as an alternative source of dietary fibre (DF). Total DF content was determined, some of its physico-chemical properties described and the physiological effects of U. fasciata meal on rats fed a hypercholesterolemic diet were investigated. U. fasciata may be considered a potential alternative source of DF with a total content of about 400 g.kg-1 (dry basis) and interesting physico-chemical properties: water reten...

Afu, Carvalho; Mcc, Portela; Mb, Sousa; Fs, Martins; Fc, Rocha; Df, Farias; Jpa, Feitosa

2009-01-01

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Physiological and physico-chemical characterization of dietary fibre from the green seaweed Ulva fasciata Delile.  

Science.gov (United States)

This work aims to assess the potential of the green seaweed Ulva fasciata Delile as an alternative source of dietary fibre (DF). Total DF content was determined, some of its physico-chemical properties described and the physiological effects of U. fasciata meal on rats fed a hypercholesterolemic diet were investigated. U. fasciata may be considered a potential alternative source of DF with a total content of about 400 g.kg-1 (dry basis) and interesting physico-chemical properties: water retention capacity of 8.74 g/water.g-1 dry sample (seaweed meal) and 0.90 (seaweed carbohydrate extract), lipid adsorption capacity of 4.52 g/oil.g-1 dry sample (seaweed meal) and 5.70 (seaweed carbohydrate extract), intrinsic viscosity of 2.4 dl.g-1 (seaweed carbohydrate extract) and cation exchange capacity of 3.51 Eq.kg-1 (seaweed carbohydrate extract). The diet containing seaweed meal was able to keep rats' total cholesterol (TC) down without causing any undesirable increase in LDL-C fraction. No evidence of toxic and/or antinutritional components in the seaweed meal was detected. Rats showed a fecal volume much greater (13 g) than that fed on cellulose diet (7 g) (p < 0.05). These properties confer on the seaweed the potential to be used in food technology for the acquisition of low-calorie food and might be important in body weight control, reduction of blood TC and LDL-C as well as in prevention of gastrointestinal diseases. PMID:19802460

Carvalho, A F U; Portela, M C C; Sousa, M B; Martins, F S; Rocha, F C; Farias, D F; Feitosa, J P A

2009-08-01

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Physiological and physico-chemical characterization of dietary fibre from the green seaweed Ulva fasciata Delile / Caracterização fisiológica e físico-química da fibra alimentar da macroalga marinha verde Ulva fasciata Delile  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Este trabalho objetivou avaliar o potencial da alga marinha verde Ulva fasciata Delile como fonte alternativa de fibra alimentar. Foram realizadas a determinação do teor de fibra alimentar total e a descrição de algumas propriedades físico-químicas, e os efeitos fisiológicos da farinha da alga seca [...] sobre ratos alimentados com dieta hipercolesterolemizante foram investigados. Esta alga pode ser considerada uma fonte alternativa potencial de fibra com cerca de 400 g.kg-1 (base seca) e propriedades físico-químicas interessantes: uma capacidade de retenção de água de 8,74 g/água.g-1 de amostra seca (farinha de alga) e 0,90 (extrato de carboidratos), uma capacidade de adsorção de lipídeos de 4,52 g/óleo.g-1 de amostra seca (farinha de alga) e 5,70 (extrato de carboidratos), uma viscosidade intrínseca de 2,4 dl.g-1 (extrato de carboidrato da alga) e capacidade de troca iônica de 3,51 Eq.kg-1 (extrato de carboidrato). A dieta contendo farinha de alga foi capaz de manter baixos os níveis de colesterol total de ratos sem causar aumento indesejável na fração LDL-C. Nenhuma evidência de componentes tóxicos e/ou antinutricionais na farinha de alga foi encontrada. Os ratos mostraram um volume fecal maior (13 g) do que aqueles alimentados com dieta contendo celulose como fonte de fibra (7 g) (p Abstract in english This work aims to assess the potential of the green seaweed Ulva fasciata Delile as an alternative source of dietary fibre (DF). Total DF content was determined, some of its physico-chemical properties described and the physiological effects of U. fasciata meal on rats fed a hypercholesterolemic die [...] t were investigated. U. fasciata may be considered a potential alternative source of DF with a total content of about 400 g.kg-1 (dry basis) and interesting physico-chemical properties: water retention capacity of 8.74 g/water.g-1 dry sample (seaweed meal) and 0.90 (seaweed carbohydrate extract), lipid adsorption capacity of 4.52 g/oil.g-1 dry sample (seaweed meal) and 5.70 (seaweed carbohydrate extract), intrinsic viscosity of 2.4 dl.g-1 (seaweed carbohydrate extract) and cation exchange capacity of 3.51 Eq.kg-1 (seaweed carbohydrate extract). The diet containing seaweed meal was able to keep rats' total cholesterol (TC) down without causing any undesirable increase in LDL-C fraction. No evidence of toxic and/or antinutritional components in the seaweed meal was detected. Rats showed a fecal volume much greater (13 g) than that fed on cellulose diet ( 7 g) (p

AFU., Carvalho; MCC., Portela; MB., Sousa; FS., Martins; FC., Rocha; DF., Farias; JPA., Feitosa.

2009-08-01

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Chemical composition of Ulvaria oxysperma (Kützing) bliding, Ulva lactuca (Linnaeus) and Ulva fascita (Delile)  

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Chemical composition of three species of Chlorophyta, Ulva lactuca, Ulva fasciata and Ulvaria oxysperma, was determined. Ulvaria oxysperma showed humidity (16-20%), ash (17-31% dry-base), proteins (6-10%db), lipids (0.5-3.2%db), fibers (3-12%db) and carbohydrates (46-72%db) which corresponded to 192-270 kcal.100g-1 (wet-base). U lactuca (15-18%db) and U fasciata (13-16%db) revealed grades slightly higher for proteins, but with similar energetic contents (250-272 and 225-239 kcal.100g-1), resp...

Márcia de Pádua; Paulo Sérgio Growoski Fontoura; Alvaro Luiz Mathias

2004-01-01

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Chemical composition of Ulvaria oxysperma (Kützing) bliding, Ulva lactuca (Linnaeus) and Ulva fascita (Delile)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese A composição química de três espécies de Chlorophyta foi determinada. Ulva lactuca se desenvolve em Guaraqueçaba e Ulva fasciata na Ilha do Mel, enquanto que Ulvaria oxysperma se desenvolve nos dois ecossistemas. Umidade (16-20%), cinzas (17-31%, base seca), proteínas (6-10%bs), lipídeos (0,5-3.2%bs [...] ), fibras (3-12%bs) e carboidratos (46-72%bs) para U.oxysperma correspondeu a 192-270 kcal.100g-1 (base úmida). U.lactuca (15-18%bs) e U.fasciata (13-16%bs) mostraram teores levemente maiores para a proteína, mas com conteúdos energéticos similares (250-272 e 225-239 kcal.100g-1), respectivamente. As folhas in natura de U.lactuca e de U.fasciata eram mais rígidas do que as de U.oxysperma. Outras algas nativas apresentaram teores de proteínas maiores. Ulvaria sp pode ser cultivada sobre redes, sem apresentar competição com outras algas ou ação significativa de predadores. Ulvaria sp cultivada apresentou composição química similar ao da Ulvaria sp crescida sobre substratos naturais. É essencial escolher um local protegido da ação destrutiva da maré. Abstract in english Chemical composition of three species of Chlorophyta, Ulva lactuca, Ulva fasciata and Ulvaria oxysperma, was determined. Ulvaria oxysperma showed humidity (16-20%), ash (17-31% dry-base), proteins (6-10%db), lipids (0.5-3.2%db), fibers (3-12%db) and carbohydrates (46-72%db) which corresponded to 192 [...] -270 kcal.100g-1 (wet-base). U lactuca (15-18%db) and U fasciata (13-16%db) revealed grades slightly higher for proteins, but with similar energetic contents (250-272 and 225-239 kcal.100g-1), respectively. Natural blades of U lactuca and of U fasciata were more rigid than blades of U oxysperma. Other native algae showed higher protein levels. Ulvaria sp could be cultivated on the cotton-nylon net without having significance competition from other algae or predators degradation. On the other hand, it was essential choose a place without destructive tide action. Cultivated Ulvaria sp showed similar chemical composition as that grown on natural substrates.

Márcia de, Pádua; Paulo Sérgio Growoski, Fontoura; Alvaro Luiz, Mathias.

2004-03-01

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Chemical composition of Ulvaria oxysperma (Kützing bliding, Ulva lactuca (Linnaeus and Ulva fascita (Delile  

Directory of Open Access Journals (Sweden)

Full Text Available Chemical composition of three species of Chlorophyta, Ulva lactuca, Ulva fasciata and Ulvaria oxysperma, was determined. Ulvaria oxysperma showed humidity (16-20%, ash (17-31% dry-base, proteins (6-10%db, lipids (0.5-3.2%db, fibers (3-12%db and carbohydrates (46-72%db which corresponded to 192-270 kcal.100g-1 (wet-base. U lactuca (15-18%db and U fasciata (13-16%db revealed grades slightly higher for proteins, but with similar energetic contents (250-272 and 225-239 kcal.100g-1, respectively. Natural blades of U lactuca and of U fasciata were more rigid than blades of U oxysperma. Other native algae showed higher protein levels. Ulvaria sp could be cultivated on the cotton-nylon net without having significance competition from other algae or predators degradation. On the other hand, it was essential choose a place without destructive tide action. Cultivated Ulvaria sp showed similar chemical composition as that grown on natural substrates.A composição química de três espécies de Chlorophyta foi determinada. Ulva lactuca se desenvolve em Guaraqueçaba e Ulva fasciata na Ilha do Mel, enquanto que Ulvaria oxysperma se desenvolve nos dois ecossistemas. Umidade (16-20%, cinzas (17-31%, base seca, proteínas (6-10%bs, lipídeos (0,5-3.2%bs, fibras (3-12%bs e carboidratos (46-72%bs para U.oxysperma correspondeu a 192-270 kcal.100g-1 (base úmida. U.lactuca (15-18%bs e U.fasciata (13-16%bs mostraram teores levemente maiores para a proteína, mas com conteúdos energéticos similares (250-272 e 225-239 kcal.100g-1, respectivamente. As folhas in natura de U.lactuca e de U.fasciata eram mais rígidas do que as de U.oxysperma. Outras algas nativas apresentaram teores de proteínas maiores. Ulvaria sp pode ser cultivada sobre redes, sem apresentar competição com outras algas ou ação significativa de predadores. Ulvaria sp cultivada apresentou composição química similar ao da Ulvaria sp crescida sobre substratos naturais. É essencial escolher um local protegido da ação destrutiva da maré.

Márcia de Pádua

2004-03-01

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Antiviral activity of the green marine alga Ulva fasciata on the replication of human metapneumovirus Atividade antiviral da alga verde marinha Ulva fasciata na replicação do metapneumovírus humano  

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We evaluated the antiviral activity of the marine alga, Ulva fasciata, collected from Rasa beach and Forno beach, Búzios, Rio de Janeiro, Brazil on the replication of human metapneumovirus (HMPV). The algae extracts were prepared using three different methodologies to compare the activity of different groups of chemical composites obtained through these different methodologies. Four out of the six extracts inhibited nearly 100% of viral replication. The results demonstrated that the majority...

Gabriella da Silva Mendes; Angélica Ribeiro Soares; Fernanda Otaviano Martins; Maria Carolina Maciel de Albuquerque; Sonia Soares Costa; Yocie Yoneshigue-Valentin; Lísia Mônica de Souza Gestinari; Norma Santos; Maria Teresa Villela Romanos

2010-01-01

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Efficacy of marine green alga Ulva fasciata extract on the management of shrimp bacterial diseases Eficacia del extracto del alga marina verde Ulva fasciata sobre el manejo de las enfermedades bacterianas en camarones  

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Secondary metabolites of the green algae, Ulva fasciata, were tested to determine the efficacy of controlling shrimp bacterial pathogens. Exploratory experiments indicated that an intermediate dose (1 g kg-1 of shrimp) of Ulva in the diet was highly effective at controlling bacterial pathogens of shrimp, as compared to lower (500 mg kg-1) and higher (1.5 g kg-1) doses. The pilot experiments evaluated the percent of relative protection afforded shrimps treated with Ulva diet and faced with var...

Joseph Selvin; Aseer Manilal; Suganthan Sujith; George Seghal Kiran; Aron Premnath Lipton

2011-01-01

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Efficacy of marine green alga Ulva fasciata extract on the management of shrimp bacterial diseases / Eficacia del extracto del alga marina verde Ulva fasciata sobre el manejo de las enfermedades bacterianas en camarones  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in spanish Metabolites secundario de algas verdes Ulva fasciata fue probado para determinar la eficacia de controlar el camarón pathogens bacterial. Las conclusiones de experimentos exploratorios indicaron que la dosis mediana (1 g kg-1 de camarón) de dieta Ulva era sumamente eficaz en el control de pathogens [...] bacterial de camarón cuando comparado al más abajo (500 mg kg-1) y más alto (1,5 g kg-1) dosis. En los experimentos pilotos, la protección de pariente de por ciento de camarones trató con la dieta Ulva y desafió con varias concentraciones de bacterial patógeno fueron evaluados. La supervivencia de camarones trató con la dieta Ulva era significativo (P Abstract in english Secondary metabolites of the green algae, Ulva fasciata, were tested to determine the efficacy of controlling shrimp bacterial pathogens. Exploratory experiments indicated that an intermediate dose (1 g kg-1 of shrimp) of Ulva in the diet was highly effective at controlling bacterial pathogens of sh [...] rimp, as compared to lower (500 mg kg-1) and higher (1.5 g kg-1) doses. The pilot experiments evaluated the percent of relative protection afforded shrimps treated with Ulva diet and faced with various concentrations of bacterial pathogen. The survival of shrimps treated with Ulva diet was significant (P

Joseph, Selvin; Aseer, Manilal; Suganthan, Sujith; George, Seghal Kiran; Aron, Premnath Lipton.

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Efficacy of marine green alga Ulva fasciata extract on the management of shrimp bacterial diseases / Eficacia del extracto del alga marina verde Ulva fasciata sobre el manejo de las enfermedades bacterianas en camarones  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in spanish Metabolites secundario de algas verdes Ulva fasciata fue probado para determinar la eficacia de controlar el camarón pathogens bacterial. Las conclusiones de experimentos exploratorios indicaron que la dosis mediana (1 g kg-1 de camarón) de dieta Ulva era sumamente eficaz en el control de pathogens [...] bacterial de camarón cuando comparado al más abajo (500 mg kg-1) y más alto (1,5 g kg-1) dosis. En los experimentos pilotos, la protección de pariente de por ciento de camarones trató con la dieta Ulva y desafió con varias concentraciones de bacterial patógeno fueron evaluados. La supervivencia de camarones trató con la dieta Ulva era significativo (P Abstract in english Secondary metabolites of the green algae, Ulva fasciata, were tested to determine the efficacy of controlling shrimp bacterial pathogens. Exploratory experiments indicated that an intermediate dose (1 g kg-1 of shrimp) of Ulva in the diet was highly effective at controlling bacterial pathogens of sh [...] rimp, as compared to lower (500 mg kg-1) and higher (1.5 g kg-1) doses. The pilot experiments evaluated the percent of relative protection afforded shrimps treated with Ulva diet and faced with various concentrations of bacterial pathogen. The survival of shrimps treated with Ulva diet was significant (P

Joseph, Selvin; Aseer, Manilal; Suganthan, Sujith; George, Seghal Kiran; Aron, Premnath Lipton.

2011-07-01

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FACILE GREEN SYNTHESIS OF GOLD NANOPARTICLES WITH GREAT CATALYTIC ACTIVITY USING ULVA FASCIATA  

Directory of Open Access Journals (Sweden)

Full Text Available We report a facile, green, and high yielding approache for the synthesis and stabilization of monodisperse gold nanoparticles (AuNPs using green seaweed Ulva fasciata extract. Characterization of the obtained AuNPs was performed using UV-visible, Fourier transform infrared (FTIR, X-ray diffraction (XRD and transmission electron microscopy (TEM. UV-visible absorption spectroscopy was used to determine the yield of the gold nanoparticles. The UV-visible absorption spectrum showed a characteristic optical peak of AuNPs at 541 nm. The X-ray diffraction pattern suggested the formation and crystallinity of AuNPs. Spherical AuNPs synthesized with an average particle size of 10 ± 3 nm were confirmed by TEM. FTIR analysis supported the role of phytochemicals of Ulva fasciata extract for bioreduction and stabilization of AuNPs. Moreover, the synthesized AuNPs exhibit remarkable catalytic efficiency by using the reduction of 4-nitroaniline by potassium borohydride in aqueous solution using UV-visible absorption spectroscopy. Catalytic reduction followed pseudo-first-order kinetics with respect to 4-Nitrophenol.

V. Sugantha Kumari

2014-03-01

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Development and application of a marine sediment pore-water toxicity test using Ulva fasciata zoospores  

Science.gov (United States)

An acute (96 h) pore-water toxicity test protocol using germination and growth of Ulva fasciata zoospores as endpoints was developed to test the toxicity of marine and estuarine sediment pore-water samples. Tests with an organic toxicant (sodium dodecyl sulfate; SDS), three metals (Cd, Cu, and Zn), and ammonia (NH3) were conducted to determine zoospore sensitivity. Zoospore germination and gametophyte growth were as sensitive to SDS as sea urchin (Arbacia punctulata) fertilization and embryological development. Zoospore sensitivity to metals was greater than or comparable to that of adult macroalgae. Zoospores were less sensitive to NH3 than were other commonly used toxicity test organisms. Test results using this algal assay with sediment pore-water samples with high NH3 concentrations were compared with results from sea urchin fertilization and embryological development tests for the same samples. Ulva fasciata zoospore germination was not affected by samples with high NH3 concentrations that were toxic in both sea urchin tests. Zoospore tolerance of NH3 and sensitivity to other contaminants indicate that their response may be useful in toxicity identification evaluation studies with pore-water samples that contain high concentrations of unionized NH3.

Hooten, R.L.; Carr, R.S.

1998-01-01

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Development and application of a marine sediment pore-water toxicity test using Ulva fasciata zoospores  

Energy Technology Data Exchange (ETDEWEB)

An acute (96 h) pore-water toxicity test protocol using germination and growth of Ulva fasciata zoospores as endpoints was developed to test the toxicity of marine and estuarine sediment pore-water samples. Tests with an organic toxicant (sodium dodecyl sulfate; SDS), three metals (Cd, Cu, and Zn), and ammonia (NH{sub 3}) were conducted to determine zoospore sensitivity. Zoospore germination and gametophyte growth were as sensitive to SDS as sea urchin (Arbacia punctulata) fertilization and embryological development. Zoospore sensitivity to metals was greater than or comparable to that of adult macroalgae. Zoospores were less sensitive to NH{sub 3} than were other commonly used toxicity test organisms. Test results using this algal assay with sediment pore-water samples with high NH{sub 3} concentrations were compared with results from sea urchin fertilization and embryological development tests for the same samples. Ulva fasciata zoospore germination was not affected by samples with high NH{sub 3} concentrations that were toxic in both sea urchin tests. Zoospore tolerance of NH{sub 3} and sensitivity to other contaminants indicate that their response may be useful in toxicity identification evaluation studies with pore-water samples that contain high concentrations of unionized NH{sub 3}.

Hooten, R.L.; Carr, R.S. [Texas A and M Univ., Corpus Christi, TX (United States). Geological Survey

1998-01-01

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Antiviral activity of the green marine alga Ulva fasciata on the replication of human metapneumovirus / Atividade antiviral da alga verde marinha Ulva fasciata na replicação do metapneumovírus humano  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Neste artigo, foi avaliada a atividade antiviral da alga marinha Ulva fasciata, coletada nas Praias do Forno e Rasa, em Búzios, Rio de Janeiro, Brasil, sobre a replicação do metapneumovírus humano (HMPV). Os extratos desta alga foram preparados utilizando três diferentes metodologias, visando a comp [...] aração da atividade de diferentes grupos de compostos químicos que são obtidos dependendo da metodologia empregada. Quatro, do total de seis extratos foram capazes de inibir praticamente 100% da replicação viral. Os resultados demonstram também que a maioria dos extratos (cinco, dos seis), possui atividade virucida e, portanto, possuem a habilidade de interagir com a partícula viral extracelularmente impedindo a infecção. Por outro lado, apenas dois extratos (coletado da Praia do Forno e, preparado através de maceração e maceração do decocto) foram capazes de se ligar a receptores celulares, impossibilitando assim a entrada das partículas virais nas células. Finalmente, apenas o extrato que foi preparado por maceração da alga coletada na Praia do Forno, demonstrou atividade intracelular. Até onde sabemos, este é um estudo pioneiro sobre a atividade antiviral de algas marinhas sobre o HMPV. É também o primeiro estudo sobre atividade antiviral sobre HMPV realizado no Brasil. O estudo também mostra o efeito de diferentes condições ambientais e procedimentos químicos utilizados na preparação do extrato sobre suas propriedades biológicas. Abstract in english We evaluated the antiviral activity of the marine alga, Ulva fasciata, collected from Rasa beach and Forno beach, Búzios, Rio de Janeiro, Brazil on the replication of human metapneumovirus (HMPV). The algae extracts were prepared using three different methodologies to compare the activity of differe [...] nt groups of chemical composites obtained through these different methodologies. Four out of the six extracts inhibited nearly 100% of viral replication. The results demonstrated that the majority of the extracts (five out of six) possess virucidal activity and therefore have the ability to interact with the extracellular viral particles and prevent the infection. On the other hand, only two extracts (from Forno beach, obtained by maceration and maceration of the decoction) were able to interact with cell receptors, hindering the viral entry. Finally, only the extract of algae collected at Forno beach, obtained by maceration presented intracellular activity. To our knowledge, this is a pioneer study on antiviral activity of marine algae against HMPV. It is also the first on antiviral activity against HMPV ever done in Brazil. The study also shows the effect of different environment factors and different chemical procedures used to obtain the extract on its biological properties.

Gabriella da Silva, Mendes; Angélica Ribeiro, Soares; Fernanda Otaviano, Martins; Maria Carolina Maciel de, Albuquerque; Sonia Soares, Costa; Yocie, Yoneshigue-Valentin; Lísia Mônica de Souza, Gestinari; Norma, Santos; Maria Teresa Villela, Romanos.

2010-02-01

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Rheology and characteristics of sulfated polysaccharides from chlorophytan seaweeds Ulva fasciata.  

Science.gov (United States)

The rheological characteristics of polysaccharides which were extracted and separated from Ulva fasciata (UFP) were investigated in aqueous solutions under conditions of concentration, temperature, solution pH and salt concentrations. It was described by the power-law model with a consistency index (k) and a flow behavior index (n). The rheology results showed UFP exhibited as a shear-thickening fluid and a possible mechanism was proposed to explain this phenomenon that might be the collapse of UFP necklace-type structures. UFP characteristics were evaluated by determining the chemical analysis and zeta potential. The findings indicated UFP may consist of partially ulvan, as the results were in accordance with the ulvan structure. Additionally, a rod-climbing effect and cold-set gelation were observed in the UFP semidilute solution. Therefore, the cold-set gelling properties and unique shear-thickening fluid properties in this work could be valuable for the exploration of U. fasciata as a new source of water-soluble gelling polysaccharides. PMID:25256496

Shao, Ping; Qin, Minpu; Han, Longfei; Sun, Peilong

2014-11-26

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Effects of partial desulfation on antioxidant and inhibition of DLD cancer cell of Ulva fasciata polysaccharide.  

Science.gov (United States)

Ulva fasciata belonging to the family Ulvaceae, commonly known as 'sea lettuce', is an abundantly growing green seaweed in coastal seashore of South China. Three different molecular weight sulfated polysaccharides (UFP1, UFP2 and UFP3) were extracted and separated from U. fasciata by hot water extraction and ultrafiltration. The three native UFP fractions had partial desulfurization by solvolytic desulfation respectively and the effect of sulfate content on the exhibition of the antioxidant and anti-tumor capacities had been evaluated and compared. The results showed that each native polysaccharide (UFP1, UFP2, UFP3) with high sulfate content exhibited better antioxidant activities compared with the partial desulfated polysaccharides (DS-UFP1, DS-UFP2, DS-UFP3). Specifically, UFP2 with relatively high sulfate content, molecular weight and uronic acid content had consistently excellent antioxidant performances. However, UFP2 demonstrated the minimal inhibitory effects on growth of DLD intestinal cancer cells. Instead, DS-UFP3 with the lowest sulfate content but highest uronic acid content and molecular weight exhibited the best antitumor activity. PMID:24463264

Shao, Ping; Pei, Yaping; Fang, Zhongxian; Sun, Peilong

2014-04-01

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Death by Ulva  

Science.gov (United States)

We report on a series of field and laboratory mesocosm experiments where we examined the effects of two levels of decomposing Ulva on Spartina alterniflora growth, soil biogeochemistry, and nitrogen dynamics. Monitoring of porewater revealed rapid mineralization to ammonium from...

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Photosynthetic properties of three Brazilian seaweeds Propriedades fotossintéticas de três macroalgas marinhas brasileiras  

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Photosynthetic performance of distinct marine macroalgae, Ulva fasciata Delile (green alga), Lobophora variegata (J. V. Lamouroux) Womersley ex E. C. Oliveira (brown alga), and Plocamium brasiliensis (Greville) M. A. Howe & W. R. Taylor (red alga), were compared using a pulse amplitude-modulated fluorometer. The maximum quantum yield (Fv/Fm) ranged from 0.80 to 0.51, and the lowest value was found in P. brasiliensis. Under 400 µmol photons m-2 s-1 irradiance, the highest value of photochemic...

Chaloub, Ricardo M.; Fernanda Reinert; Nassar, Cristina A. G.; Fleury, Beatriz G.; Mantuano, Dulce G.; Larkum, Anthony W. D.

2010-01-01

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[Parthenogenetic generations of Helicometra fasciata Rud., 1819 (Trematoda: Opecoelidae) in the Black Sea molluscs Gibbula adriatica].  

Science.gov (United States)

Morphology of the Helicometra fasciata Rud., 1819 parthenogenetic generation from the Black Sea gastropods Gibbula adriatica (Phil.) was studied for the first time. Data on seasonal dynamics of the hemipopulation of daughter sporocysts are given. Daughter sporocysts of H. fasciata infest 10 +/- 0.2 % of G. adriatica (mainly specimens of larger size and elder age classes). As a rule, local microhemipopulations of daughter sporocysts castrate mollusc hosts. Reproduction of H. fasciata daughter sporocysts is asynchronous: daughter sporocysts born specimens of next sporocyst generation during autumn and winter, and then they begin producing cercaria. In winter development of the cercaria embryo is blocked. Second change of the character of the each sporocyst' posterity is impossible because of the annual life cycle of G. adriatica. Endogenous agglomeration of the H. fasciata daughter sporocysts is extremely little: individuals of next sporocyst generation develop from no more than 2 % of embryonic balls. Energy resources of the mollusc host are used by the H. fasciata daughter sporocysts mainly for producing cercaria; this fact can be interpreted as an adaptation of H. fasciata to using medium-sized, short-living mollusc hosts. PMID:18543805

Korni?chuk, Iu M

2008-01-01

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Energy production from marine biomass (Ulva lactuca)  

Energy Technology Data Exchange (ETDEWEB)

In this project, methods for producing liquid, gaseous and solid biofuel from the marine macroalgae Ulva lactuca has been studied. To get an understanding of the growth conditions of Ulva lactuca, laboratory scale growth experiments describing N, P, and CO{sub 2} uptake and possible N{sub 2}O and CH{sub 4} production are carried out. The macroalgae have been converted to bioethanol and methane (biogas) in laboratory processes. Further the potential of using the algae as a solid combustible biofuel is studied. Harvest and conditioning procedures are described together with the potential of integrating macroalgae production at a power plant. The overall conclusions are: 1. Annual yield of Ulva lactuca is 4-5 times land-based energy crops. 2. Potential for increased growth rate when bubbling with flue gas is up to 20%. 3. Ethanol/butanol can be produced from pretreated Ulva of C6 and - for butanol - also C5 sugars. Fermentation inhibitors can possibly be removed by mechanical pressing. The ethanol production is 0,14 gram pr gram dry Ulva lactuca. The butanol production is lower. 4. Methane yields of Ulva are at a level between cow manure and energy crops. 5. Fast pyrolysis produces algae oil which contains 78 % of the energy content of the biomass. 6. Catalytic supercritical water gasification of Ulva lactuca is feasible and a methane rich gas can be obtained. 7. Thermal conversion of Ulva is possible with special equipment as low temperature gasification and grate firing. 8. Co-firing of Ulva with coal in power plants is limited due to high ash content. 9. Production of Ulva only for energy purposes at power plants is too costly. 10. N{sub 2}O emission has been observed in lab scale, but not in pilot scale production. 11. Analyses of ash from Ulva lactuca indicates it as a source for high value fertilizers. 12. Co-digestion of Ulva lactuca together with cattle manure did not alter the overall fertilization value of the digested cattle manure alone. (LN)

Nikolaisen, L.; Daugbjerg Jensen, P.; Svane Bech, K. [Danish Technological Institute (DTI), Taastrup (Denmark)] [and others

2011-11-15

 
 
 
 
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Photosynthèse et nutrition carbonée chez ULVA SP  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Laboratory experiments have been conducted in order to examine the grouth of ULVA SP. Results show that growth suddenly stops at a precise moment. Collectively, the results of simultaneously analysis of culture media constituents demonstrate that several components may alter the growth process of UIva sp. Based on the culture experiments, we argue that the gowth responses of UIva sp. are unaffected by pH or oxygen concentrations, but field plants of ULVA sp. are libely to be ponctually limite...

Piriou, Jean-yves; Duval, Vale?rie

1990-01-01

22

Essential oil constituents of Eclipta prostrata (L.) L. and Vernonia amygdalina Delile.  

Science.gov (United States)

The chemical composition of the essential oils from the leaves and stem bark of Eclipta prostrata (L.) L. and the aerial parts of Vernonia amygdalina Delile (Asteraceae) have been analyzed by capillary gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The investigation led to the identification of 33 and 30 compounds in the oils of the leaves and stem of E. prostrate, respectively, and 40 compounds in the oil of V. amygdalina. While the oil of the leaves of E. prostrata was highly dominated by sesquiterpenoids (89.3%), the stem bark was comprised of sesquiteprenoids (47.7%), straight chain hydrocarbons (25.6%) and monoterpenoids (11.1%). The main constituents of both oils were P-caryophyllene (47.7% and 15.9%) and a-humulene (31.8 and 12.9%) in the leaves and stem, respectively. In addition, (E)-beta-farnesene (10.0%) was also identified in significant amount in the stem bark. On the other hand, the major component of V. amygdalina oil was alpha-muurolol (45.7%). PMID:19413125

Ogunbinu, Akinola O; Flamini, Guido; Cioni, Pier L; Ogunwande, Isiaka A; Okeniyi, Sunday O

2009-03-01

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CRYSTALS BANDS ON THE SURFACE OF FRESHWATER ULVA TAXA  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The incrustation process is an important occurrence whose effects were noticeable in the changes in growth of species from genera Ulva. It consisted in the precipitation of calcium carbonate on the surface of the cells wall of the freshwater macrogreen alga. The calcium carbonate occurred abundantly and formed characteristic bands on the surface of Ulva thalli. Surface incrustation of calcite was clearly rugged. In the first phase of development Ulva thalli growth naturally, could reach up to...

Messyasz, Beata; Rybak, Andrzej; Pikosz, Marta

2013-01-01

24

Physiological and Biochemical Responses of Ulva prolifera and Ulva linza to Cadmium Stress  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Responses of Ulva prolifera and Ulva linza to Cd2+ stress were studied. We found that the relative growth rate (RGR), Fv/Fm, and actual photochemical efficiency of PSII (Yield) of two Ulvaspecies were decreased under Cd2+ treatments, and these reductions were greater in U. prolifera than in U. linza. U. prolifera accumulated more cadmium than U. linza under Cd2+ stress. While U. linza showed positive osmotic adjustment ability (OAA) at a wider Cd2+ range than U. prolifera. U. linza had greate...

Jiang, He-ping; Gao, Bing-bing; Li, Wen-hui; Zhu, Ming; Zheng, Chun-fang; Zheng, Qing-song; Wang, Chang-hai

2013-01-01

25

Anatomical characters of the medicinal leaf and stem of Gymnanthemum amygdalinum (Delile) Sch.Bip. ex Walp. (Asteraceae)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Gymnanthemum amygdalinum (Delile) Sch.Bip. ex Walp. (Asteraceae), espécie mais conhecida pela sinonímia Vernonia amygdalina Delile, é um pequeno arbusto empregado na medicina popular como antipirético, laxativo, antimalárico e anti-helmíntico. Estudos demonstraram que diferentes extratos do vegetal [...] possuem atividades antioxidante, antimicrobiana e antiparasitária. Entre os metabólitos bioativos presentes, citam-se lactonas sesquiterpenoides, saponinas, polifenóis e flavonoides. Este estudo investigou os caracteres microscópicos de folha e caule de G. amygdalinum, a fim de ampliar o conhecimento acerca dessa espécie medicinal e apontar estruturas anatômicas características. O material foi fixado e seccionado à mão livre e em micrótomo. Os cortes foram corados ou submetidos aos testes histoquímicos clássicos. Empregou-se microscopia eletrônica de varredura para investigar a ultraestrutura da superfície epidérmica. A folha é anfiestomática com estômatos anomocíticos. Há cutícula estriada, tricomas glandular e tector e mesofilo dorsiventral. Em secção transversal, a nervura central e o pecíolo têm contorno plano-convexo. Ambos mostram vários feixes vasculares colaterais e poucos cristais de oxalato de cálcio. No caule, a epiderme permanece e o felogênio tem instalação periférica. São observadas endoderme típica e calotas esclerenquimáticas apostas ao floema. Os aspectos que fornecem elementos para a identificação da espécie são a ocorrência de estômatos em ambas as superfícies foliares, a organização da nervura central e do pecíolo, a endoderme e as calotas esclerenquimáticas no caule, além dos diferentes tipos de tricoma nos órgãos aéreos estudados. Abstract in english Gymnanthemum amygdalinum (Delile) Sch.Bip. ex Walp. (Asteraceae), better known by its former name Vernonia amygdalina Delile, is a small shrub used in folk medicine as an antipyretic, laxative, antimalarial and anthelmintic. Studies have demonstrated that different vegetal extracts possess antioxida [...] nt, antimicrobial and antiparasitic activities. Among the bioactive metabolites, there are sesquiterpene lactones, saponins, polyphenols and flavonoids. This study investigated the leaf and stem microscopic characters of G. amygdalinum, aiming to expand the knowledge on this medicinal species and indicate anatomical structures. Plant material was fixed and sectioned by freehand and using a microtome. The sections were either stained or underwent standard histochemical tests. Scanning electron microscopy was performed to investigate epidermal relief. The leaf is amphistomatic with anomocytic stomata. There are striate cuticle, glandular and non-glandular trichomes and dorsiventral mesophyll. In transverse section, the midrib and the petiole have a plano-convex shape. Both show several collateral vascular bundles and few crystals of calcium oxalate. In the stem, the epidermis persists and the phellogen has a peripheral origin. It presents typical endodermis and sclerenchymatic caps adjoining the phloem. The aspects that contribute to characterizing the species are stomata on both leaf surfaces, midrib and petiole features, the endodermis and sclerenchymatic caps in the stem, as well as the different types of trichome on both aerial organs.

Márcia do Rocio, Duarte; Ariane Gonçalves, Silva.

26

Anatomical characters of the medicinal leaf and stem of Gymnanthemum amygdalinum (Delile) Sch.Bip. ex Walp. (Asteraceae)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Gymnanthemum amygdalinum (Delile) Sch.Bip. ex Walp. (Asteraceae), espécie mais conhecida pela sinonímia Vernonia amygdalina Delile, é um pequeno arbusto empregado na medicina popular como antipirético, laxativo, antimalárico e anti-helmíntico. Estudos demonstraram que diferentes extratos do vegetal [...] possuem atividades antioxidante, antimicrobiana e antiparasitária. Entre os metabólitos bioativos presentes, citam-se lactonas sesquiterpenoides, saponinas, polifenóis e flavonoides. Este estudo investigou os caracteres microscópicos de folha e caule de G. amygdalinum, a fim de ampliar o conhecimento acerca dessa espécie medicinal e apontar estruturas anatômicas características. O material foi fixado e seccionado à mão livre e em micrótomo. Os cortes foram corados ou submetidos aos testes histoquímicos clássicos. Empregou-se microscopia eletrônica de varredura para investigar a ultraestrutura da superfície epidérmica. A folha é anfiestomática com estômatos anomocíticos. Há cutícula estriada, tricomas glandular e tector e mesofilo dorsiventral. Em secção transversal, a nervura central e o pecíolo têm contorno plano-convexo. Ambos mostram vários feixes vasculares colaterais e poucos cristais de oxalato de cálcio. No caule, a epiderme permanece e o felogênio tem instalação periférica. São observadas endoderme típica e calotas esclerenquimáticas apostas ao floema. Os aspectos que fornecem elementos para a identificação da espécie são a ocorrência de estômatos em ambas as superfícies foliares, a organização da nervura central e do pecíolo, a endoderme e as calotas esclerenquimáticas no caule, além dos diferentes tipos de tricoma nos órgãos aéreos estudados. Abstract in english Gymnanthemum amygdalinum (Delile) Sch.Bip. ex Walp. (Asteraceae), better known by its former name Vernonia amygdalina Delile, is a small shrub used in folk medicine as an antipyretic, laxative, antimalarial and anthelmintic. Studies have demonstrated that different vegetal extracts possess antioxida [...] nt, antimicrobial and antiparasitic activities. Among the bioactive metabolites, there are sesquiterpene lactones, saponins, polyphenols and flavonoids. This study investigated the leaf and stem microscopic characters of G. amygdalinum, aiming to expand the knowledge on this medicinal species and indicate anatomical structures. Plant material was fixed and sectioned by freehand and using a microtome. The sections were either stained or underwent standard histochemical tests. Scanning electron microscopy was performed to investigate epidermal relief. The leaf is amphistomatic with anomocytic stomata. There are striate cuticle, glandular and non-glandular trichomes and dorsiventral mesophyll. In transverse section, the midrib and the petiole have a plano-convex shape. Both show several collateral vascular bundles and few crystals of calcium oxalate. In the stem, the epidermis persists and the phellogen has a peripheral origin. It presents typical endodermis and sclerenchymatic caps adjoining the phloem. The aspects that contribute to characterizing the species are stomata on both leaf surfaces, midrib and petiole features, the endodermis and sclerenchymatic caps in the stem, as well as the different types of trichome on both aerial organs.

Márcia do Rocio, Duarte; Ariane Gonçalves, Silva.

2013-12-01

27

The feeding ecology of Bonelli’s eagle (Aquila fasciata) floaters in southern Spain: implications for conservation  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Abstract Although many studies have investigated the feeding habits of Palaearctic raptors, few have analysed non-breeding populations during dispersal. Bonelli’s eagle (Aquila fasciata), a threatened species in Western Europe, has a relatively long and critical dispersal period. We studied feeding habits, prey selection, and the influence of prey density on floater abundance in this species during its dispersal period in southern Spain. Differences were found between the diet of...

2011-01-01

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Intra-organismal distribution of tetrodotoxin in two species of blue-ringed octopuses (Hapalochlaena fasciata and H. lunulata).  

Science.gov (United States)

In-depth studies on the intra-organismal distribution of toxin may yield valuable clues about potential ecological functions. The distribution of tetrodotoxin (TTX) in previously unexamined tissues of two species of blue-ringed octopuses, wild-caught Hapalochlaena fasciata and Hapalochlaena lunulata from the aquarium industry, was surveyed. Tissues from each individual were examined separately. Tetrodotoxin was detected in posterior salivary gland (PSG), arm, mantle, anterior salivary glands, digestive gland, testes contents, brachial heart, nephridia, gill, and oviducal gland of H. fasciata. By contrast TTX was found only in the PSG, mantle tissue, and ink of H. lunulata. The highest concentrations of TTX resided in the PSG of both species; however, the arms and mantle contained the greatest absolute amounts of TTX. Minimum total amounts of TTX per octopus ranged from 60 to 405 microg in H. fasciata and from 0 to 174 microg in H. lunulata and correlated well with the amounts in the PSG. Transport of TTX in the blood is loosely suggested by the presence of the toxin in blood-rich organs such as the gill and brachial hearts. The distributional data also suggest both offensive and defensive functions of TTX. PMID:19481562

Williams, Becky L; Caldwell, Roy L

2009-09-01

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OCCURRENCE OF MACROALGAE FROM THE ULVA GENERA (ULVACEAE; CHLOROPHYTA) IN THE WIELKOPOLSKA REGION  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The work presents the specification of the reports from the appearance of species from Ulva genera (Ulvaceae, Chlorophyta) in the Wielkopolska region. The stands of the three species (Ulva compressa, U. intestinalis and U. prolifera) were marked on the map of the region in the ATPOL squares sequence. Since seventies till 2008 thalluses of the Ulva genera have been noticed in the 10 stands. Ulva species occurred in the lakes, ponds and rivers localized in the central part of the Wielkopolska r...

Rybak, Andrzej; Messyasz, Beata

2009-01-01

30

Onchocerca fasciata Railliet and Henry, 1910 and its nodule development in camels in Saudi Arabia.  

Science.gov (United States)

A total of 192 male camels of three age groups (young, adult and old) from Saudi Arabia were examined for Onchocerca fasciata infection by detection of microfilariae in skin snips and nodules in the nuchal ligaments and subcutaneous tissues of the neck and shoulder. The overall prevalence rates were 10.9 and 33.3%, respectively. The prevalence rate by the skin snip technique and the number of microfilariae per gram of skin were higher in young and adult camels than in old camels. However, the prevalence rate by the detection of nodules and the number of nodules per infected camel, increased with increase in age of the camels. An increase in size and weight of nodules was reported with an increase in age of the camels. Nodules varied in diameter from 2 to 36 mm and in weight from 0.5 to 5.0 g. The overall percentage of soft viable and calcified nodules was 42.5 and 57.5%, respectively. The viability of worms decreased, but calcification increased with increased age of the camels. Four levels of degeneration and calcification of worms were described following scanning electron microscopy. PMID:1897120

Ghandour, A M; al-Amoudi, A A; Banaja, A A

1991-07-01

31

Reproducción y dimorfismo sexual en una población de Homonota fasciata (Squamata: Phyllodactylidae) del monte de San Juan, Argentina / Reproduction and sexual dimorphism in a population of Homonota fasciata (Squamata: Phyllodactylidae) from Mount of San Juan, Argentina  

Scientific Electronic Library Online (English)

Full Text Available SciELO Mexico | Language: Spanish Abstract in spanish El objetivo de este trabajo fue conocer y describir el ciclo reproductivo y el dimorfismo sexual morfométrico de Homonota fasciata en el monte de San Juan. Para analizar la reproducción, se midió en hembras la longitud y ancho de folículos yemados y/o huevos en oviducto; en machos se midió el ancho [...] y largo de testículos, con el fin de estimar sus volúmenes. El dimorfismo sexual se examinó midiendo 10 variables morfométricas en 49 individuos (26 hembras y 23 machos). Homonota fasciata inició su actividad reproductiva en primavera para los 2 años de registro. El tamaño de camada fue de un huevo. En hembras, se evidenció al menos una triple postura y el ciclo gonadal varió con las estaciones. En machos, el desarrollo testicular no exhibió variaciones, demostrando una condición reproductiva continua a través de la estación de actividad. Se halló dimorfismo sexual en 2 variables: longitud radio-cúbito, siendo mayor en hembras que en machos; y longitud tibia-fíbula, siendo esta variable mayor en machos y atribuyendo el dimorfismo encontrado a la marcada territorialidad de la especie. Este estudio es un valioso aporte al conocimiento de la biología de la especie, en un contexto tanto ecológico como de conservación. Abstract in english The aim of this study was to know and describe the reproductive cycle and sexual dimorphism of Homonota fasciata form the Mount of San Juan. To analyze reproduction, females were measured in the length and breadth of yemados follicles and/or eggs in the oviduct, in males testes length and width were [...] measured, in order to estimate their volumes. Sexual dimorphism was examined measuring 10 morphometric variables in 49 individuals (26 females and 23 males). Homonota fasciata started their reproductive activity in spring for 2 years recorded. The smallest size was 1 egg. Females, showed at least a triple posture and the gonadal cycle varied with seasons. In males, testicular development did not exhibit variations, showing a continuous reproductive condition through the activity season. We found sexual dimorphism in 2 variables: radio-ulna length, being higher in females than in males, and tibia-fibula length, being this variable major in males and attributing dimorphism found to the marked the territoriality of the species. This study is a valuable contribution to the knowledge of this species in a context of ecology and conservation.

Rodrigo A., Nieva; Graciela M., Blanco; Juan C., Acosta; Matías, Olmedo.

1266-12-01

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TEMPORAL VARIATION IN THE ABUNDANCE AND DISTRIBUTION OF POECILIOPSIS FASCIATA IN NEAR-SHORE HABITAT OF THE HIGH ELEVATION LAKE, LAGO DE ACHICHILCA, PUEBLA, MEXICO  

Directory of Open Access Journals (Sweden)

Full Text Available The distribution of organisms within a pond or lake can reflect the result of a variety of factors. We examined the distribution and abundance of the fish, Poecilioposis fasciata, in Lago de Achichilca, Puebla, Mexico, as well as how the distribution and abundance varied among months. Dissolved oxygen, temperature, and salinity varied among months. The abundance of P. fasciata peaked in December and February. For the months when fish were observed, their abundances were positively related to dissolved oxygen concentration and were generally not related to temperature. Our results make it clear that there is substantial seasonal variation in the abundances of P. fasciata and that within months, their distributions are likely driven more by dissolved oxygen than either temperature or salinity.

Montoya-Ayala Raymundo

2012-04-01

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Homonota fasciata Duméril y Bibron, 1839 (Reptilia, Squamata, Phyllodactylidae. Primer registro para la provincia de Corrientes (República Argentina  

Directory of Open Access Journals (Sweden)

Full Text Available Homonota fasciata Duméril & Bibron, 1839. República Argentina, Provincia de Corrientes, Departamento Bella Vista, Cuarta Sección, Colonia Progreso, Loma Sur, Toropí (28°35'55" S; 59°03'38" W (Figura 1. Colectores: Eduardo Etchepare, María del Rosario Ingaramo, Camila Falcione y Roberto Aguirre. 05 de abril de 2011. Se colectaron 4 ejemplares adultos (2 machos y 2 hembras y 1 juvenil, depositados en la Colección Herpetológica de la Universidad Nacional del Nordeste (UNNEC-11212, UNNEC-11213, UNNEC- 11214, UNNEC-11215 y UNNEC-11216.

Falcione, Camila

2011-05-01

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Photosynthetic properties of three Brazilian seaweeds / Propriedades fotossintéticas de três macroalgas marinhas brasileiras  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese O desempenho fotossintético de três grupos distintos de macroalgas marinhas, Ulva fasciata Delile (alga verde), Lobophora variegata (J. V. Lamouroux) Womersley ex E. C. Oliveira (alga parda) e Plocamium brasiliensis (Greville) M. A. Howe & W. R. Taylor (alga vermelha), foi comparado com auxílio de u [...] m fluorímetro de pulso e amplitude modulada. O potencial fotoquímico máximo do PS II (Fv/Fm) variou de 0,80 a 0,51, sendo que os menores valores foram observados em P. brasiliensis. Sob a irradiância de 400 µmol fótons m-2 s-1, o maior valor de dissipação fotoquímica (qP = 0,92 ± 0,13) foi observado para U. fasciata. A alga vermelha P. brasiliensis dissipou elevada quantidade de energia de excitação (qN = 0,56 ± 0,09), resultando em valores baixos de potencial fotoquímico efetivo do PS II (0,23 ± 0,04), e também de taxa relativa de transporte de elétrons (3,3 ± 0,7). O elevado potencial fotossintético encontrado para U. fasciata explica, parcialmente, a capacidade da espécie de crescimento rápido e de alta produtividade. Abstract in english Photosynthetic performance of distinct marine macroalgae, Ulva fasciata Delile (green alga), Lobophora variegata (J. V. Lamouroux) Womersley ex E. C. Oliveira (brown alga), and Plocamium brasiliensis (Greville) M. A. Howe & W. R. Taylor (red alga), were compared using a pulse amplitude-modulated flu [...] orometer. The maximum quantum yield (Fv/Fm) ranged from 0.80 to 0.51, and the lowest value was found in P. brasiliensis. Under 400 µmol photons m-2 s-1 irradiance, the highest value of photochemical quenching (qP = 0.92 ± 0.13) was observed for U. fasciata. The red alga P. brasiliensis dissipated high amounts of excitation energy (qN = 0.56 ± 0.09), resulting in relatively low values for the effective quantum yield of PS-II (0.23 ± 0.04), as well as for the relative electron transport rate (3.3 ± 0.7). The high photosynthetic potential found for U. fasciata partially explains the species ability for rapid growth and high productivity.

Ricardo M., Chaloub; Fernanda, Reinert; Cristina A. G., Nassar; Beatriz G., Fleury; Dulce G., Mantuano; Anthony W. D., Larkum.

2010-06-01

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Interference with the germination and growth of Ulva zoospores by quorum-sensing molecules from Ulva-associated epiphytic bacteria.  

Science.gov (United States)

Ulva zoospores preferentially settle on N-acylhomoserine lactone (AHL) producing marine bacterial biofilms. To investigate whether AHL signal molecules also affect the success and rate of zoospore germination in addition to zoospore attraction, the epiphytic bacteria associated with mature Ulva linza were characterized and bacterial isolates representative of this community tested for the ability to produce AHLs. Two of these AHL-producing isolates, Sulfitobacter spp. 376 and Shewanella spp. 79, were transformed with plasmids expressing the Bacillus spp. AHL lactonase gene aiiA to generate AHL-deficient variants. The germination and growth of U.?linza zoospores was studied in the presence of these AHL-deficient strains and their AHL-producing counterparts. This revealed that the AHLs produced by Sulfitobacter spp. and Shewanella spp. or the bacterial products they regulate have a negative impact on both zoospore germination and the early growth of the Ulva germling. Further experiments with Escherichia coli biofilms expressing recombinant AHL synthases and synthetic AHLs provide data to demonstrate that zoospores germinated and grown in the absence of AHLs were significantly longer than those germinated in the presence of AHLs. These results reveal an additional role for AHLs?per se in the interactive relationships between marine bacteria and Ulva zoospores. PMID:23879807

Twigg, Matthew S; Tait, Karen; Williams, Paul; Atkinson, Steve; Cámara, Miguel

2014-02-01

36

Nutritional value of green seaweed (Ulva lactuca) for broiler chickens  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The current work aimed to assess the potential of the green seaweed Ulva lactuca (U. lactuca) as an alternative ingredient in broiler chicken diets. The effect of substituting 1.0 or 3.0% of corn with U. lactuca on performance, carcass characteristics, serum constituents and nutrients retention of broilers from 12 to 33 d of age was evaluated. Three treatments were distributed in a RCBD design: T1 = control diet (0% U. lactuca); T2 = 1....

Al-haidary, Ahmad A.; Samara, Emad M.; Abdoun, Kalid A.; Aljumaah, Riyadh S.; Okab, Aly B.; Abudabos, Alaeldein M.

2013-01-01

37

Strong Endemism of Bloom-Forming Tubular Ulva in Indian West Coast, with Description of Ulva paschima Sp. Nov. (Ulvales, Chlorophyta).  

Science.gov (United States)

Ulva intestinalis and Ulva compressa are two bloom-forming morphologically-cryptic species of green seaweeds widely accepted as cosmopolitan in distribution. Previous studies have shown that these are two distinct species that exhibit great morphological plasticity with changing seawater salinity. Here we present a phylogeographic assessment of tubular Ulva that we considered belonging to this complex collected from various marine and estuarine green-tide occurrences in a ca. 600 km stretch of the Indian west coast. Maximum Likelihood and Bayesian Inference phylogenetic reconstructions using ITS nrDNA revealed strong endemism of Indian tubular Ulva, with none of the Indian isolates forming part of the already described phylogenetic clades of either U. compressa or U. intestinalis. Due to the straightforward conclusion that Indian isolates form a robust and distinct phylogenetic clade, a description of a new bloom-forming species, Ulva paschima Bast, is formally proposed. Our phylogenetic reconstructions using Neighbor-Joining method revealed evolutionary affinity of this new species with Ulva flexuosa. This is the first molecular assessment of Ulva from the Indian Subcontinent. PMID:25329833

Bast, Felix; John, Aijaz Ahmad; Bhushan, Satej

2014-01-01

38

Adsorption of uranium(VI) onto Ulva sp. -sepiolite composite  

International Nuclear Information System (INIS)

Ulva sp. and sepiolite were used to prepare composite adsorbent. The adsorption of uranium(VI) from aqueous solutions onto Ulva sp.-sepiolite has been studied by using a batch adsorber. The parameters that affect the uranium(VI) adsorption, such as solution pH, initial uranium(VI) concentration, and temperature, have been investigated and the optimum conditions determined. The adsorption patterns of uranium on the composite adsorbent followed the Freundlich and Dubinin-Radushkevich (D-R) isotherms. The Freundlich, Langmuir, and Dubinin-Radushkevich (D-R) models have been applied and the data correlate well with Freundlich model. The sorption is physical in nature (sorption energy, E = 4.01 kJ/mol). The thermodynamic parameters such as variation of enthalpy ?H, variation of entropy ?S and variation of Gibbs free energy ?G were calculated from the slope and intercept of lnKd vs. 1/T plots. Thermodynamic parameters (?Hads = -22.17 kJ/mol, ?Sads = -17.47 J/mol x K, ?Gdegads (298.15 K) = -16.96 kJ/mol) show the exothermic heat of adsorption and the feasibility of the process. The results suggested that the Ulva sp-sepiolite composite adsorbent is suitable as a sorbent material for recovery and biosorption/adsorption of uranium ions from aqueous solutions. (author)

39

Electroantennogram responses of the tea slug moth, Iragoides fasciata to some plant volatiles associated with tea, Camellia sinensis.  

Science.gov (United States)

Electroantennogram responses to a wide range of plant volatile compounds that have been identified in tea plants Camellia sinensis L. (Ericales: Theaceae) were recorded from males and females of the tea slug moth, Iragoides fasciata Moore (Lepidoptera: Limacodidae). The responses to 26 compounds, belonging to several chemical classes, and two mixtures were evaluated. The results showed significantly different electroantennogram responses to the different chemicals, as well as significantly different responses according to gender. The green leaf volatile components elicited significantly greater responses in males. In general, the antennae of males were more sensitive, and responded more strongly, to most of the compounds. Responses to sesquiterpenoids were lower in both males and females. Dose-dependent response studies indicated differences in response between genders and concentrations, suggesting the existence of sexual dimorphism. Compounds belonging to the green leaf volatiles class appeared to be important clues in host-plant selection by this oligophagous species. PMID:23414096

Huang, An-Ping; Bao, Xiao-Cun; Liu, Ben-Ying; Wang, Yuan-Jiang; Zhou, Ling-Yun; Ning, Jing; Han, Bao-Yu

2012-01-01

40

Microdistribution of tetrodotoxin in two species of blue-ringed octopuses (Hapalochlaena lunulata and Hapalochlaena fasciata) detected by fluorescent immunolabeling.  

Science.gov (United States)

Blue-ringed octopuses (genus Hapalochlaena) possess the potent neurotoxin tetrodotoxin (TTX). We examined the microdistribution of TTX in ten tissues of Hapalochlaena lunulata and Hapalochlaena fasciata by immunolabeling for fluorescent light microscopy (FLM). We visualized TTX throughout the posterior salivary gland, but the toxin was concentrated in cells lining the secretory tubules within the gland. Tetrodotoxin was present just beneath the epidermis of the integument (mantle and arms) and also concentrated in channels running through the dermis. This was suggestive of a TTX transport mechanism in the blood of the octopus, which would also explain the presence of the toxin in the blood-rich brachial hearts, gills, nephridia, and highly vascularized Needham's sac (testes contents). We also present the first report of TTX in any cephalopod outside of the genus Hapalochlaena. A specimen of Octopus bocki from French Polynesia contained a small amount of TTX in the digestive gland. PMID:22983011

Williams, Becky L; Stark, Michael R; Caldwell, Roy L

2012-12-01

 
 
 
 
41

Sodium and potassium uptake of Ulva - Application of marine macro-algae for space agriculture  

Science.gov (United States)

The treatment of sodium, Na, and potassium, K, presents a challenge in space agriculture material recycling, as humans require Na and plants cannot grow at high Na concentrations. Hence, we are proposing the use of marine macro-algae to harvest K and other minerals from composted human waste. Ulva was selected for this feasibility study, since it tolerates a wide range of salinity levels. Growth capability of Ulva was examined under various total salinity levels and proportions of Na and K in the incubation medium. A homeostatic feature of Ulva was found in its intra-cellular concentration of Na and K, and in the intra-cellular ratio between Na and K (at 0.58 ± 0.30, lower than that of human metabolic waste). Intracellular concentration of K in Ulva is 20 times higher than seawater. Because of these characteristics, Ulva is a good candidate species for space agriculture.

Yamashita, M.; Tomita-Yokotani, K.; Hashimoto, H.; Sawaki, N.; Notoya, M.

2009-04-01

42

Alterações na composição florística das algas da Praia de Boa Viagem (Niterói, RJ) / Changes in the marine flora of the Boa Viagem beach (Niterói, RJ)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese A Baía de Guanabara no decorrer da ocupação de suas margens sofreu uma continua degradação, levando-a a grandes modificações em seu entorno e a inúmeros danos ambientais. Além de uma significativa alteração paisagística, ocorreu também uma queda na qualidade de suas águas, devido ao lançamento de gr [...] ande quantidade de efluentes não tratados (domésticos e industriais). Este trabalho teve como objetivo inventariar a flora algácea da praia de Boa Viagem (Niterói, RJ), compará-la com levantamentos realizados há três décadas. Os resultados mostraram desaparecimento de 30 táxons (49%) outrora existentes no início da década de 70 e o aparecimento de 14 espécies (23%). A flora atual é dominada por Ulva fasciata Delile e Enteromorpha compressa (L.) Nees, espécies potencialmente indicadoras de poluição orgânica. Tais resultados evidenciam que, ao longo dessas três décadas, uma série de impactos trouxe profundas alterações na comunidade de macroalgas. Abstract in english During the past three decades, the Guanabara Bay suffered continuous degradation, leading to severe changes in the surrounding area and environmental damages. Besides significant loss of its natural landscape, the quality of its water decreased with continuous disposal of non-treated domestic organi [...] c sewage and industrial effluents. The present study aimed to survey the algal flora of Boa Viagem beach (Niterói, RJ) and to compare it with information gathered three decades ago. Results showed a decrease of 30 taxa (49%) of the algal species and an increase of 14 species (23%) in comparison with algal flora recorded three decades ago. The actual algal flora is dominated by Ulva fasciata Delile and Enteromorpha compressa (L.) Nees, species indicative of organic pollution. These results elucidate the environmental impacts caused upon the macroalgae community of the Guanabara Bay during throughout the period.

ANDRÉ, TAOUIL; YOCIE, YONESHIGUE-VALENTIN.

2002-12-01

43

Alterações na composição florística das algas da Praia de Boa Viagem (Niterói, RJ Changes in the marine flora of the Boa Viagem beach (Niterói, RJ  

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Full Text Available A Baía de Guanabara no decorrer da ocupação de suas margens sofreu uma continua degradação, levando-a a grandes modificações em seu entorno e a inúmeros danos ambientais. Além de uma significativa alteração paisagística, ocorreu também uma queda na qualidade de suas águas, devido ao lançamento de grande quantidade de efluentes não tratados (domésticos e industriais. Este trabalho teve como objetivo inventariar a flora algácea da praia de Boa Viagem (Niterói, RJ, compará-la com levantamentos realizados há três décadas. Os resultados mostraram desaparecimento de 30 táxons (49% outrora existentes no início da década de 70 e o aparecimento de 14 espécies (23%. A flora atual é dominada por Ulva fasciata Delile e Enteromorpha compressa (L. Nees, espécies potencialmente indicadoras de poluição orgânica. Tais resultados evidenciam que, ao longo dessas três décadas, uma série de impactos trouxe profundas alterações na comunidade de macroalgas.During the past three decades, the Guanabara Bay suffered continuous degradation, leading to severe changes in the surrounding area and environmental damages. Besides significant loss of its natural landscape, the quality of its water decreased with continuous disposal of non-treated domestic organic sewage and industrial effluents. The present study aimed to survey the algal flora of Boa Viagem beach (Niterói, RJ and to compare it with information gathered three decades ago. Results showed a decrease of 30 taxa (49% of the algal species and an increase of 14 species (23% in comparison with algal flora recorded three decades ago. The actual algal flora is dominated by Ulva fasciata Delile and Enteromorpha compressa (L. Nees, species indicative of organic pollution. These results elucidate the environmental impacts caused upon the macroalgae community of the Guanabara Bay during throughout the period.

ANDRÉ TAOUIL

2002-12-01

44

Alterações na composição florística das algas da Praia de Boa Viagem (Niterói, RJ) / Changes in the marine flora of the Boa Viagem beach (Niterói, RJ)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese A Baía de Guanabara no decorrer da ocupação de suas margens sofreu uma continua degradação, levando-a a grandes modificações em seu entorno e a inúmeros danos ambientais. Além de uma significativa alteração paisagística, ocorreu também uma queda na qualidade de suas águas, devido ao lançamento de gr [...] ande quantidade de efluentes não tratados (domésticos e industriais). Este trabalho teve como objetivo inventariar a flora algácea da praia de Boa Viagem (Niterói, RJ), compará-la com levantamentos realizados há três décadas. Os resultados mostraram desaparecimento de 30 táxons (49%) outrora existentes no início da década de 70 e o aparecimento de 14 espécies (23%). A flora atual é dominada por Ulva fasciata Delile e Enteromorpha compressa (L.) Nees, espécies potencialmente indicadoras de poluição orgânica. Tais resultados evidenciam que, ao longo dessas três décadas, uma série de impactos trouxe profundas alterações na comunidade de macroalgas. Abstract in english During the past three decades, the Guanabara Bay suffered continuous degradation, leading to severe changes in the surrounding area and environmental damages. Besides significant loss of its natural landscape, the quality of its water decreased with continuous disposal of non-treated domestic organi [...] c sewage and industrial effluents. The present study aimed to survey the algal flora of Boa Viagem beach (Niterói, RJ) and to compare it with information gathered three decades ago. Results showed a decrease of 30 taxa (49%) of the algal species and an increase of 14 species (23%) in comparison with algal flora recorded three decades ago. The actual algal flora is dominated by Ulva fasciata Delile and Enteromorpha compressa (L.) Nees, species indicative of organic pollution. These results elucidate the environmental impacts caused upon the macroalgae community of the Guanabara Bay during throughout the period.

ANDRÉ, TAOUIL; YOCIE, YONESHIGUE-VALENTIN.

45

Morphology and reproduction of Asiatic Ulva pertusa (Ulvales, Chlorophyta) in Thau Lagoon (France, Mediterranean Sea)  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Une étude détaillée des stades végétatif et fertile de plusieurs populations d'une espèce d'Ulva commune dans l'étang de Thau (France, Méditerranée) a montré qu'elle appartenait à l'espèce asiatique Ulva pertusa Kjellman. Depuis octobre 1994, date de sa première observation dans l'étang de Thau, U. pertusa a développé des populations importantes et fertiles. L'espèce a probablement été introduite à Thau avec des importations d'huîtres en provenance du Pacifique. Ulva pert...

Verlaque, Marc; Belsher, Thomas; Deslous-paoli, Jean-marc

2002-01-01

46

Trichomonas stableri n. sp., an agent of trichomonosis in Pacific Coast band-tailed pigeons (Patagioenas fasciata monilis).  

Science.gov (United States)

Trichomonas gallinae is a ubiquitous flagellated protozoan parasite, and the most common etiologic agent of epidemic trichomonosis in columbid and passerine species. In this study, free-ranging Pacific Coast band-tailed pigeons (Patagioenas fasciata monilis) in California (USA) were found to be infected with trichomonad protozoa that were genetically and morphologically distinct from T. gallinae. In microscopic analysis, protozoa were significantly smaller in length and width than T. gallinae and were bimodal in morphology. Phylogenetic analysis of the ITS1/5.8S/ITS2, rpb1, and hydrogenosomal Fe-hydrogenase regions revealed that the protozoan shares an ancestor with Trichomonas vaginalis, the sexually-transmitted agent of trichomoniasis in humans. Clinical and pathologic features of infected birds were similar to infections with T. gallinae. Evidence presented here strongly support taxonomical distinction of this parasite, which we hereby name Trichomonas stableri n. sp. This work contributes to a growing body of evidence that T. gallinae is not the sole etiologic agent of avian trichomonosis, and that the incorporation of molecular tools is critical in the investigation of infectious causes of mortality in birds. PMID:24918075

Girard, Yvette A; Rogers, Krysta H; Gerhold, Richard; Land, Kirkwood M; Lenaghan, Scott C; Woods, Leslie W; Haberkern, Nathan; Hopper, Melissa; Cann, Jeff D; Johnson, Christine K

2014-04-01

47

Flat-plate techniques for measuring reflectance of macro-algae (Ulva curvata)  

Science.gov (United States)

We tested the consistency and accuracy of flat-plate spectral measurements (400–1000 nm) of the marine macrophyte Ulva curvata. With sequential addition of Ulva thallus layers, the reflectance progressively increased from 6% to 9% with six thalli in the visible (VIS) and from 5% to 19% with ten thalli in the near infrared (NIR). This progressive increase was simulated by a mathematical calculation based on an Ulva thallus diffuse reflectance weighted by a transmittance power series. Experimental and simulated reflectance differences that were particularly high in the NIR most likely resulted from residual water and layering structure unevenness in the experimental progression. High spectral overlap existed between fouled and non-fouled Ulva mats and the coexistent lagoon mud in the VIS, whereas in the NIR, spectral contrast was retained but substantially dampened by fouling.

Ramsey, Elijah W., III; Rangoonwala, Amina; Thomsen, Mads Solgaard; Schwarzschild, Arthur

2012-01-01

48

Morphological and ultrastructural studies on Ulva flexuosa subsp. pilifera (Chlorophyta from Poland  

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Full Text Available Ulva flexuosa subsp. pilifera (Kütz. M. J. Wynne 2005 (= Enteromorpha pilifera Kützing 1845 was previously found in Argentina, the Czech Republic, Germany, Hungary, Romania, Slovakia and Sweden, recently also in Poland. The genus Ulva was first time described as Enteromorpha. Interestingly, Enteromorpha is used nowadays as a synonym for Ulva, a development which is based on molecular data. The morphologies of both young and mature specimens were studied, and most life cycle stages could be observed. Further, the formation of calcium carbonate crystals on the surface of Ulva thalli seems to influence the arrangement of the cells. A detailed ultrastructural (TEM analysis of cell walls is presented. The TEM reveals in great details highly complex, irregular structures with stratification lines.

Beata Messyasz

2013-06-01

49

Methods for the induction of reproduction in a tropical species of filamentous ulva.  

Science.gov (United States)

The green seaweed Ulva is a major fouling organism but also an edible aquaculture product in Asia. This study quantified for the first time the effect of key factors on the reproduction of a tropical species of filamentous Ulva (Ulva sp. 3). The controlled timing of release of swarmers (motile reproductive bodies) was achieved when experiments were initiated in the early afternoon by exposing the thalli to a temperature shock (4°C) for 10 min and subsequently placing them into autoclaved filtered seawater under a 12 h light: 12 h dark photoperiod at 25°C. The release of swarmers then peaked two days after initiation. In contrast, segmentation, dehydration, salinity or time of initiation of experiments had no effect of any magnitude on reproduction. The released swarmers were predominantly biflagellate (95%), negatively phototactic and germinated without complementary gametes. This indicates that Ulva sp. 3 has a simple asexual life history dominated by biflagellate zoids. PMID:24824896

Carl, Christina; de Nys, Rocky; Lawton, Rebecca J; Paul, Nicholas A

2014-01-01

50

Effect of background colour on growth and adhesion strength of Ulva sporelings.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

This study examined the effects of a range of black, grey and white substrata on the growth and attachment strength of Ulva sporelings on glass and polydimethylsiloxane (Silastic-T2) surfaces. The rate of development of sporelings was strongly influenced by the colour of the substratum on which they grew. On black backgrounds, sporelings grew slowly and germination was delayed. Laboratory screening methods for antifouling and fouling-release coatings that rely on the growth of Ulva sporelings...

Finlay, Ja; Fletcher, Br; Callow, Me; Callow, Ja

2008-01-01

51

The signal based relationship between the green seaweed Ulva and its indigenous bacterial community  

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This project has focused on the relationship between the green seaweed Ulva, commonly found in the intertidal zone of the UK coastline and its cognate bacterial community. It has previously been reported that motile Ulva zoospores are attracted to N-Acylhomoserine lactones (AHLs), signalling molecules utilised by Gram-negative bacteria in a density dependent form of cellular communication termed quorum sensing (QS) and produced by several biofilm dwelling species of marine bacteria. The speci...

Twigg, Matthew

2013-01-01

52

Abiotic factors affecting the development of Ulva sp. (Ulvophyceae; Chlorophyta) in freshwater ecosystems  

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The influence of physicochemical factors on the development of Ulva species with distromatic tubular morphology was studied in three streams located in Poznan, Poland. The study evaluated key environmental factors that may influence the colonisation and growth of Ulva populations in freshwater systems. In total, nine environmental parameters were included: temperature, water depth, pH, oxygen (O2), ammonium (NH4),nitrate (NO3-), phosphate(PO43-), sodium chloride (NaCl) and total iron (Fe). Mo...

Messyasz, Beata; Rybak, Andrzej

2010-01-01

53

Fertility effects on Ulva thalli mass development in inland waters of Poland  

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The genus Ulva (Ulvophyceae, Chlorophyta) consists of some 140 species which are present mainly in marine waters. Nine species occur in the inland waters in Poland (central Europe), of which U. flexuosa subsp. pilifera (Kützing) Bliding is the most widespread in the Wielkopolska region. The occurrence of Ulva species in any particular locality seems to be governed mainly by the availability of nutrients (N, P) than by the high levels of anthropogenic salinity in freshwaters. Fres...

Messyasz, Beata; Rybak, Andrzej; Pikosz, Marta; Szendzina, Lidia

2013-01-01

54

Isolation Bacterial Symbiont from Ulva reticulata and Caulerpa racemosa as Candidate of Antimicrobial Producer  

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Isolation of bacterial symbionts from Ulva reticulata and Caulerpa racemosa was performed by crowded plate technique method using marine agar media. The purpose of this study was to search for antimicrobial-producing bacterial symbiont in green alga Ulva reticulata and Caulerpa racemosa from Takalar Coast, South Sulawesi. The isolates of bacterial symbiont were fermented in production media for seven days at 37oC. Antimicrobial activity was done by agar diffusion method using Bacillus subti...

Sartini; Suryadi; Nur Afni; Ayu Permata Sari

2013-01-01

55

EPIPHYTIC DIATOM COMMUNITY AND CALCIUM CARBONATE CRYSTALS CHARACTERISTICS ON THE SURFACE OF FRESHWATER Ulva THALLI  

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On the surface of the freshwater form of Ulva thalli the incrustations process takes place which resulted in the precipitate of calcium carbonate crystals (CaCO3 ). At the same time, this area is also inhabited by large numbers of periphytic communities, mainly by diatoms. Siliceous frustules and calcite crystals are responsible for the thalli roughness in all stages of its growth. Diatom communities colonizing young, thin thallus of Ulva were dominated by Cocconeis pla...

Messyasz, Beata; Pikosz, Marta; Rybak, Andrzej; ?epkowska, Karolina

2012-01-01

56

Isolation Becterial Symbiont from Ulva reticulata and Caulerpa racemosa as Candidate of Antimicrobial Producer  

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Isolation of bacterial symbionts from Ulva reticulate and Caulerpa racemosa was performed by crowded plate technique method using marine agar media. The purpose of this study was to search for antimicrobial-producing bacterial symbiont in green alga Ulva reticulate and caulerpa racemosa from takalar coast, south Sulawesi. The isolaties of bacterial symbiont were fermented in production media for seven days at 37???C. Antimicrobial activity was done by agar diffusion method using Bacillus subt...

Sartini; suryadi; Afni, Nur; Permata Sari, Ayu

2012-01-01

57

Cross-kingdom signalling: exploitation of bacterial quorum sensing molecules by the green seaweed Ulva  

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The green seaweed Ulva has been shown to detect signal molecules produced by bacteria. Biofilms that release N-acylhomoserine lactones (AHLs) attract zoospores—the motile reproductive stages of Ulva. The evidence for AHL involvement is based on several independent lines of evidence, including the observation that zoospores are attracted to wild-type bacteria that produce AHLs but are not attracted to mutants that do not produce signal molecules. Synthetic AHL also attracts zoospores and the...

Joint, Ian; Tait, Karen; Wheeler, Glen

2007-01-01

58

Purification and characterization of Ulva pertusa Kjellm alkaline phosphatase.  

Science.gov (United States)

The activity of alkaline phosphatase (ALP, EC 3.1.3.1.) was found in seaweeds, including five kinds of green alga, eighteen kinds of red alga, and six kinds of brown alga, collected from the seaside of Dalian in China. The enzyme was purified 1230-fold from Ulva pertusa Kjellm. It had a specific activity of 48.6 U/mg protein and was proven to be homogeneous by SDS-PAGE with a subunit molecular mass of 19.5 kDa. The activity of ALP peaked at pH9.8, and was completely inhibited by DTT and partly by NBS. The Michaelis-Menten constant Km and the maximum reaction velocity Vmax, at pH 9.8 and 37 degrees C were 0.950 mM and 5.00 microM/min, respectively. PMID:12784882

Yang, Dong; Wang, Jingyun; Bao, Yongming; An, Lijia

2003-05-01

59

Structural analysis of immunostimulating sulfated polysaccharides from Ulva pertusa.  

Science.gov (United States)

Sulfated polysaccharides were extracted from Ulva pertusa and fractionated to obtain the most immunostimulating fraction (F(2)). The glycosidic linkages of the polysaccharides in the fraction F(2) were determined using GC-MS and 2D-NMR spectroscopy after chemical modifications, including reduction and desulfation under various conditions. Methanol was used as a sulfate acceptor for the removal of sulfates from the polysaccharides. When desulfation was carried out at 120°C, the sulfates were removed upto 90.1% from the F(2) fraction without considerable backbone degradation. The GC-MS analysis as well as NMR spectra revealed that the backbone of the polysaccharides was mainly composed of ?-(1?4)-L-rhamnopyranosyl, ?-(1?4)-D-glucuronosyl, ?-(1?2)-L-rhamnopyranosyl, and ?-(1?4)-D-xylopyranosyl residues with branches at O-2 position of rhamnose. The sulfate groups were mostly found on glucuronic acid at O-3 position. PMID:23023040

Tabarsa, Mehdi; Lee, Sung-Joon; You, SangGuan

2012-11-01

60

The influence of sulfathiazole on the macroalgae Ulva lactuca.  

Science.gov (United States)

Sulfonamides (SA) are a class of antibiotics routinely found in environmental matrices and therefore their role as contaminants should be investigated in non-target organisms. With this purpose the present experimental work has evaluated the exposure of the chlorophycean Ulva lactuca L. to sulfathiazole (STZ), a SA drug commonly used in aquaculture, at two concentrations representing prophylactic (25 ?g mL(-1)) and therapeutic (50 ?g mL(-1)) administrations. Results showed that STZ exhibits high stability in seawater with only 18% degradation over the 5d assay at both dosages tested. Also, macroalgae demonstrated an efficient uptake capacity with constant internal concentrations after 24h regardless of the external solutions and thus should be considered as a bioindicator species in risk assessment. Both STZ concentrations induced a slight inhibition of the macroalgae growth after 96 h. PMID:24393561

Leston, Sara; Nunes, Margarida; Viegas, Ivan; Nebot, Carolina; Cepeda, Alberto; Pardal, Miguel Ângelo; Ramos, Fernando

2014-04-01

 
 
 
 
61

Ekologia i zmienno?? morfologiczno-molekularna taksonów z rodzaju Ulva (Ulvaceae, Chlorophyta) w ekosystemach s?odkowodnych Polski  

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W ekosystemach ?ródl?dowych Polski od po?owy XIX wieku obserwowano wyst?powanie makroglonów reprezentuj?cych kosmopolityczny rodzaj Ulva (Ulvaceae, Chlorophyta). Taksony z tego rodzaju pojawia?y si? najcz??ciej efemerycznie i cz?sto tworzy?y „zakwity”. Wyst?powanie gatunków Ulva w ?ródliskach, torfiankach i w wodach solnisk ?ródl?dowych t?umaczono wysokim zasoleniem wód tego typu uk?adów. Jednak?e notowano równie? plechy Ulva w jeziorach, rzekach i strumieniach,...

Rybak, Andrzej Stanis?aw

2014-01-01

62

[Research on the characteristics of Ulva. Prolifera in Shandong Peninsula during 2008-2012 based on MODIS data].  

Science.gov (United States)

In the present study, the MODIS data were used to monitor the situation of Ulva. prolifera in the Shandong Peninsula waters during the period of 2008-2012. Those studies mainly calculate the area of NDVI, and get the information of the time, area , scope , floating path of Ulva prolifera by using threshold segmentation method. The feasibility of monitoring Ulva. prolifera information based on MODIS data and the macroscopic regularity of the outburst of Ulva. prolifera was elementally studied. The results showed that Ulva. prolifera first generated in the middle of May or early June, the time, area, scope of Ulva. prolifera reached a maximum, but the relative crowding density was earlier or later when Ulva. prolifera developed into a outburst. Finally, Ulva. prolifera died away after existing for 71 days in the late July or the early August. Wholly, the floating path moved to the northwest from off the coast to offshore. Based on those aspects above, the outburst of Ulva. prolifera in 2008 and 2009 was more serious than others. PMID:25095429

Wu, Meng-Quan; Guo, Hao; Zhang, An-Ding; Jia, Li-Li; Xiao, Lu-Xiang; Wang, Jing-Pu

2014-05-01

63

Effects of UV-B radiation on the growth interaction of Ulva pertusa and Alexandrium tamarense.  

Science.gov (United States)

Enhanced UV-B (280 - 320 nm) radiation resulting from ozone depletion is one of global environmental problems. Not only marine organisms but also marine ecosystems can be affected by enhanced UV-B radiation. The effects of UV-B radiation on interaction of macro-algae and micro-algae were investigated using Ulva pertusa Kjellman and Alexandrium tamarense as the materials in this study. The results demonstrated that UV-B radiation could inhibit the growth of Ulva pertusa and Alexandrium tamarense when they were both mono-cultured, and the growth inhibition of algae was more significant with increasing doses of UV-B radiation. Alexandrium tamarense could inhibit the growth of Ulva pertusa in mixed culture, and the growth inhibition was more significant when increasing the initial cell density. However, Ulva pertusa could inhibit the growth of Alexandrium tamarense in early phase and stimulate the growth in latter phase when they were grown in mixed culture. Lower initial cell density (10(2) cell/ml) of Alexandrium tamarense could inhibit the growth of Ulva pertusa under UV-B radiation treatment, however, with the initial cell density increasing (10(3) and 10(4) cell/ml), the growth of Ulva pertusa was stimulated under lower dose of UV-B radiation and inhibited under higher dose of UV-B radiation by Alexandrium tamarense. Compared with that in mixed culture, Ulva pertusa showed more positive inhibition to the growth of Alexandrium tamarense under UV-B radiation treatment. PMID:16158588

C ai, Heng-Jiang; Tang, Xue-Xi; Zhang, Pei-Yu; Dong, Dong; Qu, Liang

2005-01-01

64

Existence of a deep subtidal stock of drifting Ulva in relation to intertidal algal mat developments  

Science.gov (United States)

Blooms of drifting green algae often develop in shallow coastal zones that receive significant nutrient inputs. Each spring and summer, some fifty bays and coves in Brittany (France) are affected in this way. Until now, in this region, only the algae present in the surf zone or stranded ashore, constituting an intertidal stock, have been taken into account. Another stock of algae, which was subtidal and of the same species ( Ulva spp.), was found in the Bay of Douarnenez, one of the ten areas most affected by these algal blooms. This subtidal Ulva stock was located beyond the surf zone, at depths reaching 15 m. It was about the same size as the intertidal stock, viz., a few thousand tons on average. Subtidal Ulva stocks were generally found lying on the sandy bottom in a distribution showing no particular pattern. Biomass ranged from almost zero to 1.547 kg m -2 of fresh and spun-dried algae. However, at depths from 3 to 7 m they were often arranged in strips a few dm wide, due to the swell's effect. The bottom conditions of temperature, salinity, irradiance and dissolved inorganic nitrogen measured during spring and summer are suitable for the growth of Ulva in the subtidal zone. Both intertidal and subtidal drifting Ulva stocks are mobile and capable of exchanging material. In spring, the intertidal stock's inoculum is likely to come from the subtidal. Later in the season, the subtidal stock could be supplied, at least partially, by the intertidal.

Merceron, M.; Morand, P.

2004-11-01

65

Effects of Tissue Nitrogen and Media Nitrate on Trace Metal Uptake and Trophic Transfer by Ulva spp.  

Science.gov (United States)

A general survey of trace metal content in Ulva spp. (Linnaeus) around Moss Landing, California was carried out. The next objective was to evaluate whether tissue nitrogen or media nitrate affects metal uptake (As, Pb, Mn, Zn) by Ulva spp. under eutrophic...

S. M. Sankaran

2012-01-01

66

Análise da contaminação fúngica em amostras de Cassia acutifolia Delile (sene e Peumus boldus (Molina Lyons (boldo-do-Chile comercializadas na cidade de Campinas, Brasil Analysis of the fungal contamination in Cassia acutifolia Delile (sene and Peumus boldus (Molina Lyons (boldo-do-Chile sold in Campinas, Brazil  

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Full Text Available A sociedade atual tem buscado a fitoterapia como um importante recurso terapêutico, sendo a avaliação da qualidade microbiológica destes produtos um requisito essencial, considerando a sua origem. Deste modo, o objetivo da pesquisa foi realizar a contagem e a identificação de fungos filamentosos em 20 amostras de folhas de Cassia acutifolia Delile (sene e de Peumus boldus (Molina Lyons (boldo-do-Chile comercializadas em farmácias de manipulação e mercados da cidade de Campinas, Brasil, usando as técnicas microbiológicas clássicas. Os resultados obtidos evidenciaram que 45% das amostras analisadas se situavam fora dos padrões estabelecidos pela Organização Mundial da Saúde (OMS. Não foram observadas diferenças significativas na análise quantitativa da contaminação fúngica entre amostras comercializadas em farmácias de manipulação e mercados, tanto para o boldo-do-Chile como para a sene. Os fungos identificados nestas amostras foram: Aspergillus, Penicillium, Phoma, Cladosporium, Trichoderma, Rhizopus, Mucor, Aureobasidium pullulans, Mycelia sterilia, Acremonium e Monilia sitophila. Estes resultados demonstraram o baixo nível de qualidade desses fitoterápicos, pois, além do elevado número de amostras contaminadas, foram identificados fungos de gêneros produtores de micotoxinas, como o Aspergillus e o Penicillium. Verifica-se a urgência na realização de adequado controle de qualidade microbiológico dos fitoterápicos e de implantação de fiscalização efetiva, para garantir a segurança e eficácia destes produtos.The consumption of medicinal plants has increased during the last years. The purpose of this study was to evaluate and identify fungi specimens present in samples of Cassia acutifolia Delile (20 (sene and Peumus boldus (Molina Lyons (Boldo-do-Chile (20, that were purchased in drugstores and markets of Campinas, Brazil, by usual methods. The results showed that 45% of samples did not fit the minimum quality standards recommended by the World Health Organization (WHO. No differences were observed in the quantitative analysis between the samples sold at the drugstores and at the markets. The identified genera and species of fungi were: Aspergillus, Penicillium, Phoma, Cladosporium, Trichoderma, Rhizopus, Mucor, Aureobasidium pullulans, Mycelia sterilia, Acremonium and Monilia sitophila. Considering the level of contamination and the presence of Aspergillus and Penicillium, which are able to produce mycotoxins, there is an urgent need for quality control of the phytotherapic products in order to assure their efficacy and safety.

Liliana de O. Rocha

2004-12-01

67

Análise da contaminação fúngica em amostras de Cassia acutifolia Delile (sene) e Peumus boldus (Molina) Lyons (boldo-do-Chile) comercializadas na cidade de Campinas, Brasil / Analysis of the fungal contamination in Cassia acutifolia Delile (sene) and Peumus boldus (Molina) Lyons (boldo-do-Chile) sold in Campinas, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese A sociedade atual tem buscado a fitoterapia como um importante recurso terapêutico, sendo a avaliação da qualidade microbiológica destes produtos um requisito essencial, considerando a sua origem. Deste modo, o objetivo da pesquisa foi realizar a contagem e a identificação de fungos filamentosos em [...] 20 amostras de folhas de Cassia acutifolia Delile (sene) e de Peumus boldus (Molina) Lyons (boldo-do-Chile) comercializadas em farmácias de manipulação e mercados da cidade de Campinas, Brasil, usando as técnicas microbiológicas clássicas. Os resultados obtidos evidenciaram que 45% das amostras analisadas se situavam fora dos padrões estabelecidos pela Organização Mundial da Saúde (OMS). Não foram observadas diferenças significativas na análise quantitativa da contaminação fúngica entre amostras comercializadas em farmácias de manipulação e mercados, tanto para o boldo-do-Chile como para a sene. Os fungos identificados nestas amostras foram: Aspergillus, Penicillium, Phoma, Cladosporium, Trichoderma, Rhizopus, Mucor, Aureobasidium pullulans, Mycelia sterilia, Acremonium e Monilia sitophila. Estes resultados demonstraram o baixo nível de qualidade desses fitoterápicos, pois, além do elevado número de amostras contaminadas, foram identificados fungos de gêneros produtores de micotoxinas, como o Aspergillus e o Penicillium. Verifica-se a urgência na realização de adequado controle de qualidade microbiológico dos fitoterápicos e de implantação de fiscalização efetiva, para garantir a segurança e eficácia destes produtos. Abstract in english The consumption of medicinal plants has increased during the last years. The purpose of this study was to evaluate and identify fungi specimens present in samples of Cassia acutifolia Delile (20) (sene) and Peumus boldus (Molina) Lyons (Boldo-do-Chile) (20), that were purchased in drugstores and mar [...] kets of Campinas, Brazil, by usual methods. The results showed that 45% of samples did not fit the minimum quality standards recommended by the World Health Organization (WHO). No differences were observed in the quantitative analysis between the samples sold at the drugstores and at the markets. The identified genera and species of fungi were: Aspergillus, Penicillium, Phoma, Cladosporium, Trichoderma, Rhizopus, Mucor, Aureobasidium pullulans, Mycelia sterilia, Acremonium and Monilia sitophila. Considering the level of contamination and the presence of Aspergillus and Penicillium, which are able to produce mycotoxins, there is an urgent need for quality control of the phytotherapic products in order to assure their efficacy and safety.

Liliana de O., Rocha; Maria Magali S. R., Soares; Cristiana Leslie, Corrêa.

68

Co-biosorption of copper and glyphosate by Ulva lactuca.  

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This study investigated the adsorption of glyphosate (PMG) onto the green algae Ulva lactuca. PMG was not adsorbed by U. lactuca but PMG was adsorbed when the process was mediated by Cu(II) with molar ratios Cu(II):PMG?1.5:1. U. lactuca was characterized by water adsorption surface area, FTIR, SEM and EDS. The Langmuir and Freundlich models were applied. Results showed that the biosorption processes for copper and PMG in the presence of copper were described described by the Langmuir model (qmax=0.85±0.09 mmol g(-1), KL=0.55±0.14 l mmol(-1) and qmax=3.65±0.46 mmol g(-1), KL=0.103±0.03 l mmol(-1), respectively). Copper adsorption was greater in the presence of PMG than in the absence of the pesticide and the adsorption can only be represented by the Freundlich model (KF=0.08±0.01, 1/n=1.86±0.07). In all cases studied, the maximum metal uptake (qmax) increased with increasing pH. Surface complexes with a stoichiometry ranging from ?Cu-PMG-Cu to ?Cu-PMG-Cu3 are suggested as reaction products of the process. Due to the increasing amounts of PMG applied in Argentina, natural reservoirs present considerable amounts of this herbicide. The value of this work resides in using U. lactuca, a marine seaweed commonly found along coastlines all over the world, as a biosorbent for PMG. PMID:23376752

Trinelli, María Alcira; Areco, María Mar; Afonso, María dos Santos

2013-05-01

69

The effects of the nitrofuran furaltadone on Ulva lactuca.  

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The use of pharmaceuticals in the food production industry as prophylatic and therapeutic agents is necessary to promote animal health, but may entail significant consequences to natural ecosystems, especially in the cases of overdosing and use of banned pharmaceuticals. The vast effects that antibiotics released into the environment have on non-target organisms are already under the scope of researchers but little attention has been given to primary producers such as macroalgae. The present study assessed furaltadone's, an antibacterial agent illegally used for veterinary purposes, uptake capacity by Ulva lactuca and its effect in the growth of this cosmopolitan macroalgae. Differences in macroalgal growth were shown when submitted to prophylactic and therapeutic concentrations of furaltadone in the water (16 and 32 ?g mL?¹, respectively). The therapeutic concentration caused higher growth impairment than the prophylactic treatment did, with 87.5% and 58% reductions respectively. Furthermore, together with data collected from the accumulation assays, with values of internal concentrations as high as 18.84 ?g g?¹ WW, suggest that the macroalgae U. lactuca should be included in field surveys as a biomonitor for the detection of nitrofurans. PMID:21109284

Leston, Sara; Nunes, Margarida; Viegas, Ivan; Lemos, Marco F L; Freitas, Andreia; Barbosa, Jorge; Ramos, Fernando; Pardal, Miguel A

2011-02-01

70

Nutritional value of green seaweed (Ulva lactuca for broiler chickens  

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Full Text Available The current work aimed to assess the potential of the green seaweed Ulva lactuca (U. lactuca as an alternative ingredient in broiler chicken diets. The effect of substituting 1.0 or 3.0% of corn with U. lactuca on performance, carcass characteristics, serum constituents and nutrients retention of broilers from 12 to 33 d of age was evaluated. Three treatments were distributed in a RCBD design: T1 = control diet (0% U. lactuca; T2 = 1.0 % U. lactuca; T3 = 3.0 % U. lactuca. Cumulative feed intake (FI, body weight gain (BWG, feed conversion ratio (FCR and nutrients retention from 12 to 33 d of age were not affected by treatment (P>0.05. Birds which had received T3 had a higher dressing percentage and breast muscle yield compared to those which had received T1 or T2. Serum total lipid, cholesterol and uric acid concentrations were significantly lower in birds which had received T2 and T3 (P green seaweed (U. lactuca.

Ahmad A. Al-Haidary

2013-04-01

71

Analysis of expressed sequence tags from the Ulva prolifera (Chlorophyta)  

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In 2008, a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao. The causative species was determined to be Enteromorpha prolifera ( Ulva prolifera O. F. Müller), a familiar green macroalga along the coastline of China. Rapid accumulation of a large biomass of floating U. prolifera prompted research on different aspects of this species. In this study, we constructed a nonnormalized cDNA library from the thalli of U. prolifera and acquired 10 072 high-quality expressed sequence tags (ESTs). These ESTs were assembled into 3 519 nonredundant gene groups, including 1 446 clusters and 2 073 singletons. After annotation with the nr database, a large number of genes were found to be related with chloroplast and ribosomal protein, GO functional classification showed 1 418 ESTs participated in photosynthesis and 1 359 ESTs were responsible for the generation of precursor metabolites and energy. In addition, rather comprehensive carbon fixation pathways were found in U. prolifera using KEGG. Some stress-related and signal transduction-related genes were also found in this study. All the evidences displayed that U. prolifera had substance and energy foundation for the intense photosynthesis and the rapid proliferation. Phylogenetic analysis of cytochrome c oxidase subunit I revealed that this green-tide causative species is most closely affiliated to Pseudendoclonium akinetum (Ulvophyceae).

Niu, Jianfeng; Hu, Haiyan; Hu, Songnian; Wang, Guangce; Peng, Guang; Sun, Song

2010-01-01

72

Kinetics of stereoselective enrichment of Dechlorane Plus in Ulva Pertusa.  

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Dechlorane Plus (DP) has been detected extensively in both biotic and abiotic matrices, and stereoselective enrichment of Dechlorane Plus isomers has also been observed in organisms. In this laboratory study, Ulva pertusa were firstly exposed to known Dechlorane Plus concentrations for 21d (uptake period), and then transferred to clean seawater for 14d (depuration period) to investigate the kinetics of Dechlorane Plus isomers in U. pertusa. Dechlorane Plus isomers reached steady-state after 21d of exposure. The mean fractional abundance of syn-DP (fsyn) exceeded than that of 0d (0.23±0.02) during uptake, but decreased during depuration. This study is the first to define the kinetics of stereoselective enrichment of Dechlorane Plus isomers in U. pertusa using two-box kinetic model. The uptake rate constants of the syn- and anti-DP were 0.164±0.056d(-1) and 0.083±0.071d(-1), respectively. The depuration rate constants were 0.337±0.057d(-1) (syn-DP) and 0.236±0.095d(-1) (anti-DP), suggesting that syn-DP is eliminated quicker than anti-DP by U. pertusa. These results are consistent with observation of fsyn and stereoselective enrichment of Dechlorane Plus isomers in U. pertusa. PMID:24997969

Zhao, Lei; Gong, Ning; Mi, Dong; Luan, Chundi; Shao, Kuishuang; Jia, Hongliang; Sun, Yeqing

2014-09-01

73

Subsequent influences of feeding intact green seaweed Ulva lactuca to growing lambs on the seminal and testicular characteristics in rams.  

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The present experiment was designed to investigate the subsequent influences of supplementing different levels of intact green seaweed Ulva lactuca (0%, 3%, and 5% DM) to growing sexually immature lambs during the growth period (74 d) on the seminal and testicular characteristics of sexually mature rams. Ulva lactuca was manually collected, adequately prepared, and then incorporated into lambs' diets. Eighteen male 3-mo-old lambs of the Awassi breed with a mean BW of 22.57 kg (SD = 1.08) were randomly assigned into treatments. The obtained results indicate that offering Ulva lactuca at the level of 3% or 5% DM to lambs during the growth period had no subsequent impacts (P > 0.05) on liver and kidney functions as well as blood water balance in rams, thereby suggesting that Ulva lactuca can be safely supplemented to lambs during growing. However, our findings point out that feeding a lamb diet supplemented with intact Ulva lactuca failed to demonstrate any subsequent benefit (P > 0.05) on the growth performance, thermoregulatory responses, and plasma oxidative status in rams. Above all, it was clearly evident that supplementing intact Ulva lactuca to lambs had demonstrated subsequent negative influences (P Ulva lactuca during the growth period compared to control rams. The deleterious impacts of feeding intact Ulva lactuca on spermatogenesis and germ cell loss were proven to be attributed to the dysfunction of Sertoli cells. Collectively, these results provide novel insights on the subsequent influences of dietary supplementation of intact Ulva lactuca to lambs. The consistent evidence of profound negative impacts on seminal and testicular characteristics as well as the resulting data of no improvement of subsequent growth, thermoregulation, and plasma oxidative status in rams prompts us to tentatively recommend the avoidance of feeding intact Ulva lactuca to lambs. PMID:24146153

Samara, E M; Okab, A B; Abdoun, K A; El-Waziry, A M; Al-Haidary, A A

2013-12-01

74

Macroalga Ulva intestinalis (L.) Occurrence in freshwater ecosystems of Poland: a new locality in Wielkopolska  

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A new locality of Ulva intestinalis was found near Kr?plewo in the River Samica St?szewska located in the Wielkopolski National Park region (Wielkopolska). On the basis of Carlson's index ranges, waters of the Samica St?szewska river were qualified as eutrophic. In the river single thalluses of U. intestinalis which appeared by its banks were observed. The presence of this Ulva species thalluses in the Samica St?szewska river confirmed the results of trophy examinations of this river. ...

Messyasz, Beata; Rybak, Andrzej

2008-01-01

75

Efectos de hidrocarburos sobre la fisiología y el crecimiento de Ulva sp. (Chlorophyta)  

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Se realizaron experimentos de laboratorio para investigar las respuestas fisiológicas de una especie de Ulva. Los discos de Ulva fueron expuestos a 16 °C hasta 12 días, a emulsiones en agua de mar con cada una de las cuatro fracciones de petróleo estándar (P1, d 0,7, bp 80-110 °C; P2, d 0,73, bp 100-140 °C; P3, d 0,76, bp 140-160 °C; P4, d 0,76, bp 180-220 °C), en cada caso a una concentración de 500, 1 000, 2 000, 4 000 y 8 000 ppm ( ?/? ). Las variables fisiológicas determina...

Zambrano, J.; Carballeira, A.

2001-01-01

76

Ulva linza zoospore sensitivity to systematic variation of surface topography  

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The use of surface topographical microstructure is abundant in nature. The lotus plant uses a fractal-like topography to create a highly non-wetting surface that self-cleans as water drops take dirt particles with them as they roll off. Analysis of how topography affects surface interactions offers a unique opportunity to attack a problem that affects our economy and societal health significantly. The attachment of biological material to manmade surfaces can be looked at as fouling or directed adhesion. Marine fouling on ship hulls costs the United States $600 million each year due to increased fuel usage caused by drag. Hospital-acquired methicillin-resistant Staphylococcus aureus infections cause thousands of deaths annually as a result of colonization of hospital surfaces. The lack of biocompatible synthetic surfaces for implants such as vascular grafts lead to restenosis as cells are unable to develop a natural interaction with the graft surface. In each circumstance there is much to learn about the complicated attachment process. This work expands the investigation of the role of topography in the attachment of the green fouling algae Ulva linza to poly(dimethylsiloxane) surfaces. Spore attachment density was correlated to the Wenzel roughness ratio on low surface energy, high-modulus poly(dimethylsiloxane)-grafted-silicon topographies. The role of topography on a scale less than the size of a spore was investigated on nano-roughened poly(dimethylsiloxane) elastomer surfaces. For a specific group of patterns, the spatial distribution of spores attached to topographies was quantitatively analyzed and shown to correlate with feature dimensions.

Sheats, Julian Taylor

77

The accumulation of metal (Co, Cr, Cu, Mn and Zn) in freshwater Ulva (Chlorophyta) and its habitat  

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The possibility of using freshwater Ulva (Chlorophyta) as a bioaccumulator of metals (Co, Cr, Cu, Mn and Zn) in lake and river water was examined weekly in the summer of 2010 in three types of samples: the water, the sediment and the thalli of Ulva. Samples of freshwater Ulva were collected from two aqueous ecosystems lie 250 km away from the basin of the Baltic Sea and 53 km from each other. A flow lake located in the centre of the big city was the first water reservoir (10 sites) and second...

Rybak, Andrzej; Messyasz, Beata; ?e?ska, Bogus?awa

2013-01-01

78

[Analysis of fatty acid composition of Ulva pertusa Kjellm by gas chromatography/mass spectrometry].  

Science.gov (United States)

A method of gas chromatography/mass spectrometry (GC/MS) was established to determine the fatty acids of Ulva pertusa Kjellm. The total lipids of Ulva pertusa Kjellm were extracted using Folch method, derivatized with HCl-CH3OH solution, and analyzed by GC/MS. The fragmentation patterns and mass spectrometry characteristics of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids were analyzed and concluded by regular patterns of organic mass spectrometry. According to the database index and standard controls, twenty-four fatty acid components in Ulva pertusa Kjellm were identified, and the contents of 9,12,15-octadecatrienoic acid, 4,7,10,13-hexadecatetraenoic acid and 6,9,2,15-octadecatetraenoic acid accounted for 45.14% of the total fatty acids. The qualitative results of fatty acids in Ulva pertusa Kjellm show that it is very useful in identifying fatty acid methyl esters by characteristic ions, especially polyunsaturated fatty acid methyl esters. PMID:21046785

Lou, Qiaoming; Xu, Jie; Wang, Yuming; Xue, Changhu; Sun, Zhaomin

2010-07-01

79

USE OF ULVA LACTUCA TO DISTINGUISH PH DEPENDENT TOXICANTS IN MARINE WATERS AND SEDIMENTS  

Science.gov (United States)

Ulva lactuca (sea lettuce) is a cosmopolitan marine attached green seaweed capable of sequestering high environmental levels of ammonia. Ammonia can be acutely toxic to marine organisms and is often found in dredged sediments from highly industrial areas or from areas with high c...

80

USE OF ULVA LACTUTA TO IDENTIFY AMMONIA TOXICITY IN MARINE AND ESTUARINE SEDIMENTS  

Science.gov (United States)

Toxicity identification evaluation(TIE) methods are being developed for use with whole sediments. Although a phase I TIE method has been developed to characterize ammonia toxicity in aqueous samples using the marine macroalga Ulva lactuca, the relationship between amphipod and my...

 
 
 
 
81

The seeding and cultivation of a tropical species of filamentous Ulva for algal biomass production.  

Science.gov (United States)

Filamentous species of Ulva are ideal for cultivation because they are robust with high growth rates and maintained across a broad range of environments. Temperate species of filamentous Ulva are commercially cultivated on nets which can be artificially 'seeded' under controlled conditions allowing for a high level of control over seeding density and consequently biomass production. This study quantified for the first time the seeding and culture cycle of a tropical species of filamentous Ulva (Ulva sp. 3) and identified seeding density and nursery period as key factors affecting growth and biomass yield. A seeding density of 621,000 swarmers m(-1) rope in combination with a nursery period of five days resulted in the highest growth rate and correspondingly the highest biomass yield. A nursery period of five days was optimal with up to six times the biomass yield compared to ropes under either shorter or longer nursery periods. These combined parameters of seeding density and nursery period resulted in a specific growth rate of more than 65% day(-1) between 7 and 10 days of outdoor cultivation post-nursery. This was followed by a decrease in growth through to 25 days. This study also demonstrated that the timing of harvest is critical as the maximum biomass yield of 23.0 ± 8.8 g dry weight m(-1) (228.7 ± 115.4 g fresh weight m(-1)) was achieved after 13 days of outdoor cultivation whereas biomass degraded to 15.5 ± 7.3 g dry weight m(-1) (120.2 ± 71.8 g fresh weight m(-1)) over a longer outdoor cultivation period of 25 days. Artificially seeded ropes of Ulva with high biomass yields over short culture cycles may therefore be an alternative to unattached cultivation in integrated pond-based aquaculture systems. PMID:24897115

Carl, Christina; de Nys, Rocky; Paul, Nicholas A

2014-01-01

82

Population structure, dynamics and production of Hydrobia ulvae (Pennant) (Mollusca: Prosobranchia) along an eutrophication gradient in the Mondego estuary (Portugal)  

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Eutrophication in the Mondego estuary gave rise to qualitative changes in the benthic community, involving the replacement of eelgrass, Zostera noltii, by green algae such as Enteromorpha spp. and Ulva sp. It seems reasonable to assume that, through time, such changes may determine a selected new trophic structure. Hydrobia ulvae, a dominant species in terms of abundance and biomass, was studied with regard to life history, population dynamics and productivity in relation to changing...

Lillebø, Ana Isabel; Pardal, Miguel A?ngelo; Marques, Joa?o Carlos

1999-01-01

83

The distribution of green algae species from the Ulva genera (syn. Enteromorpha; Chlorophyta) in Polish inland waters  

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Marine algae in inland waters in Poland have been rarely recorded. The distribution of 5 species and 1 subspecies of the Ulva genus (syn. Enteromorpha, Chlorophyta) observed in different inland aquatic ecosystems is reported. The algal distribution was established on the basis of the available literature, unpublished material, and oral reports. Information about the algal morphology and habitat conditions, from all of the 58 reported locations of ulvas in Poland, were assimilated and...

Messyasz, Beata; Rybak, Andrzej

2008-01-01

84

Anaerobic digestion of Ulva sp 3. liquefaction juices extraction by pressing and a technico-economic budget  

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In many countries, the algae of "green tides" are harvested in the fight against pollution. Ulva often represents the main component of the tide, and intensive research has been conducted on the possibility to use the algae as a methanisation substrate. However, methanisation is hampered by various practical obstacles, which requires a compromise between productivity and biological yield. The process described here calls upon a pre-digestion phase of Ulva which, besides the economy of ti...

Morand, Berenice; Briand, X.; Charlier, R.

2006-01-01

85

Biomethanation potential of macroalgae Ulva spp. and Gracilaria spp. and in co-digestion with waste activated sludge  

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Biochemical methane potential of four species of Ulva and Gracilaria genus was assessed in batch assays at mesophilic temperature. The results indicate a higher specific methane production (per volatile solids) for one of the Ulva sp. compared with other macroalgae and for tests running with 2.5% of total solids (196 ± 9 L CH4 kg?1 VS). Considering that macroalgae can potentially be a post treatment of municipal wastewater for nutrients removal, co-digestion of macroalgae with waste activa...

Costa, J. C.; Gonc?alves, P. R.; Nobre, A. M.; Alves, M. M.

2012-01-01

86

How do spores select where to settle? : A holographic motility analysis of Ulva zoospores on different surfaces  

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Wie entscheiden Sporen, wo sie siedeln? - Eine holographische Bewegungsanalyse von Ulva Zoosporen an Oberflächen mit unterschiedlichen physikochemischen Eigenschaften Ulva Sporen siedeln auf Oberflächen, um dann makroskopisch sichtbare Algen zu bilden. Dieser Bewuchs auf künstlichen Oberflächen (z.B. Bootsrümpfen) verursacht enorme Kosten, z.B. durch den erhöhten Treibstoffverbrauch von Schiffen. Um die initiale Phase der Besiedlung von Oberflächen, die schließlich zu dem Bewuchs füh...

Heydt, Matthias Rudolf

2010-01-01

87

Nitrógeno y fósforo en Ulva sp. en las Rías Bajas gallegas: cambios estacionales e influencia en el crecimiento  

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La eutrofización costera es un problema de creciente preocupación y las rías gallegas son ecosistemas sensibles a este fenómeno. En este trabajo se intenta evaluar la posible limitación nutritiva en Ulva sp., un alga que forma parte de las denominadas mareas verdes. Se realizaron análisis de nitrógeno (N) y fósforo (P), así como bioensayos de crecimiento en Ulva sp. recogida mensualmente en cuatro rías de la costa gallega durante un año. Los niveles mínimos de ambos nutrientes se ...

Villares, R.; Puente, X.; Carballeira, A.

2001-01-01

88

Impact of mercury contamination on the population dynamics of Peringia ulvae (Gastropoda): Implications on metal transfer through the trophic web  

Science.gov (United States)

The effects of mercury contamination on the population structure and dynamics of the gastropod Peringia ulvae (also known as Hydrobia ulvae) and its impact on the trophic web were assessed along a mercury gradient in Ria de Aveiro (Portugal). The gastropod was revealed to be a tolerant species to the contaminant, since the highest densities, biomasses and growth productivity values were recorded at the intermediate contaminated area followed by the most contaminated one and finally the least contaminated area. P. ulvae was however negatively affected by mercury in terms of growth and life span. So, in the most contaminated area the population was characterised mainly by the presence of juveniles and young individuals. The intermediate contaminated area showed a greater equilibrium in terms of groups' proportion, being the adults the dominant set. The least contaminated area presented intermediate values. P. ulvae life spans were shortest in the most contaminated area (7-8 mo), followed by the least contaminated area (10-11 mo) and finally, the intermediate one (11-14 mo). P. ulvae revealed to be an important vehicle of mercury transfer from sediments to the trophic web, incorporating approximately 15 g of Hg, annually, in the inner area of the Laranjo Bay (0.6 Km2). Therefore, despite P. ulvae being revealed to be not a good bio-indicator of mercury contamination, since it did not suffer profound modifications in its structure and functioning, it is a crucial element in the mercury biomagnification processes throughout the food web.

Cardoso, P. G.; Sousa, E.; Matos, P.; Henriques, B.; Pereira, E.; Duarte, A. C.; Pardal, M. A.

2013-09-01

89

Effects of Ulva rigida on the Growth, Feed Intake and Body Composition of Common Carp, Cyprinus carpio L.  

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Full Text Available This study is the first trial to evaluate algae meal, Ulva rigida, as an inexpensive and locally available feed ingredient in the diet of common carp, Cyprinus carpio. Five experimental diets were supplemented with Ulva meal at 0, 5, 10, 15 and 20% (C0, U5, U10, U15 and U20, respectively to investigate the effect of replacement of wheat meal by Ulva meal for common carp, Cyprinus carpio during a 112-day growth trial. Carp fingerlings, each initially weighing 3.1 g, were stocked into 60 l-glass tanks and were fed to apparent satiation three times daily. Performances of fish fed the test diets were evaluated in terms of survival, final mean weight, percent weight gain, specific growth rate, feed conversion ratio, protein efficiency ratio and body composition. Poorest growth performance was recorded from fish fed the diet with 20% Ulva meal supplementation (U20 (p< 0.05. Fish group fed with 5% Ulva meal inclusion (U5 achieved the best growth performance (p>0.05. Results suggested that the dietary Ulva meal inclusion of 5 to 15% replacing wheat meal in carp diets could be acceptable.

Ibrahim Diler

2007-01-01

90

Tracking the algal origin of the Ulva bloom in the Yellow Sea by a combination of molecular, morphological and physiological analyses  

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Abstract In 2008, Qingdao (36°06? N, 120°25? E, P. R. China) experienced the world largest drifting macroalgal bloom composed of the filamentous macroalga Ulva prolifera. No convincing biological evidence regarding the algal source is available so far. A series of field collections of both Ulva sp. and waters in various sites along Jiangsu coasts were conducted in March to May of 2009. Density of microscopic Ulva germlings in the waters sampled from different sites ranged fro...

2010-01-01

91

Gastropod communities associated with Ulva spp. in the littoral zone in southeast Brazil / Comunidades de gasterópodos asociados con Ulva spp. en la zona litoral del sudeste de Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in spanish Las comunidades phytales están influenciadas espacial y temporalmente por parámetros físicos y biológicos cambiantes. Este estudio describe y compara los gasterópodos asociados con Ulva spp. entre sitios en la costa de Espirito Santo, con sustrato laterítico ferruginoso y con diferentes modos de exp [...] osición al oleaje. Camburi está caracterizado por la presencia de partículas de mineral de hierro. Las muestras fueron recolectadas bimensualmente. En cada sitio se realizaron cinco cuadrantes de muestreo al azar en la region intermareal. Se encontraron 2.964 individuos y 53 taxa. En Camburi, la especie dominante fue Amphitalamus valley, y en Capuba y Manguinhos fue Eulithidium affine. El análisis realizado indicó que Camburi es distinto de los otros sitios. El menor impacto de las olas y la estructura más compleja de las algas en Camburi, debido a la presencia de mineral de hierro, puede explicar esta diferencia. Abstract in english Phytal communities are characterized by spatial and temporal variation and are influenced by fluctuating biological and physical parameters. This study aimed to describe and compare the gastropods associated with Ulva spp., at three sites of the Espirito Santo coast with ferruginous laterite substra [...] te and different modes of wave exposure. Camburi is characterized by the presence of iron ore particles. Samples were collected bimonthly. At each site, five sampling quadrats were launched at random in the intertidal region. Individuals of Ulva spp. were collected (2964 individuals) and 53 taxa were found. In Camburi the dominant species was Amphitalamus vallei (Barleeidae), while in Capuba and Manguinhos Eulithidium affine (Phasianellidae) predominated. The analyses indicated that Camburi is distinct from the other sites. The lesser wave impacts and the more complex structure of the algae in Camburi, due to the presence of iron ore, may explain this variation.

Gabriela C, Zamprogno; Mércia B, Costa; Danielle C, Barbiero; Brisa S, Ferreira; Fernanda T.V.M., Souza.

92

Gastropod communities associated with Ulva spp. in the littoral zone in southeast Brazil / Comunidades de gasterópodos asociados con Ulva spp. en la zona litoral del sudeste de Brasil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in spanish Las comunidades phytales están influenciadas espacial y temporalmente por parámetros físicos y biológicos cambiantes. Este estudio describe y compara los gasterópodos asociados con Ulva spp. entre sitios en la costa de Espirito Santo, con sustrato laterítico ferruginoso y con diferentes modos de exp [...] osición al oleaje. Camburi está caracterizado por la presencia de partículas de mineral de hierro. Las muestras fueron recolectadas bimensualmente. En cada sitio se realizaron cinco cuadrantes de muestreo al azar en la region intermareal. Se encontraron 2.964 individuos y 53 taxa. En Camburi, la especie dominante fue Amphitalamus valley, y en Capuba y Manguinhos fue Eulithidium affine. El análisis realizado indicó que Camburi es distinto de los otros sitios. El menor impacto de las olas y la estructura más compleja de las algas en Camburi, debido a la presencia de mineral de hierro, puede explicar esta diferencia. Abstract in english Phytal communities are characterized by spatial and temporal variation and are influenced by fluctuating biological and physical parameters. This study aimed to describe and compare the gastropods associated with Ulva spp., at three sites of the Espirito Santo coast with ferruginous laterite substra [...] te and different modes of wave exposure. Camburi is characterized by the presence of iron ore particles. Samples were collected bimonthly. At each site, five sampling quadrats were launched at random in the intertidal region. Individuals of Ulva spp. were collected (2964 individuals) and 53 taxa were found. In Camburi the dominant species was Amphitalamus vallei (Barleeidae), while in Capuba and Manguinhos Eulithidium affine (Phasianellidae) predominated. The analyses indicated that Camburi is distinct from the other sites. The lesser wave impacts and the more complex structure of the algae in Camburi, due to the presence of iron ore, may explain this variation.

Gabriela C, Zamprogno; Mércia B, Costa; Danielle C, Barbiero; Brisa S, Ferreira; Fernanda T.V.M., Souza.

2013-11-01

93

Accumulation of Cu and Zn from antifouling paint particles by the marine macroalga, Ulva lactuca  

Energy Technology Data Exchange (ETDEWEB)

The marine macroalga, Ulva lactuca, has been exposed to different concentrations of antifouling paint particles (4-200 mg L{sup -1}) in the presence of a fixed quantity of clean estuarine sediment and its photosynthetic response and accumulation of Cu and Zn monitored over a period of 2 days. An immediate (<2 h) toxic effect was elicited under all experimental conditions that was quantitatively related to the concentration of contaminated particles present. Likewise, the rate of leaching of both Cu and Zn was correlated with the concentration of paint particles added. Copper accumulation by the alga increased linearly with aqueous Cu concentration, largely through adsorption to the cell surface, but significant accumulation of Zn was not observed. Thus, in coastal environments where boat maintenance is practiced, discarded antifouling paint particles are an important source of Cu, but not Zn, to U. lactuca. - The marine macroalga, Ulva lactuca, is able to accumulate Cu but not Zn from discarded antifouling paint particles.

Turner, Andrew, E-mail: aturner@plymouth.ac.u [School of Earth, Ocean and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Pollock, Heather [School of Earth, Ocean and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Brown, Murray T. [School of Biological Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom)

2009-08-15

94

Accumulation of Cu and Zn from antifouling paint particles by the marine macroalga, Ulva lactuca  

International Nuclear Information System (INIS)

The marine macroalga, Ulva lactuca, has been exposed to different concentrations of antifouling paint particles (4-200 mg L-1) in the presence of a fixed quantity of clean estuarine sediment and its photosynthetic response and accumulation of Cu and Zn monitored over a period of 2 days. An immediate (<2 h) toxic effect was elicited under all experimental conditions that was quantitatively related to the concentration of contaminated particles present. Likewise, the rate of leaching of both Cu and Zn was correlated with the concentration of paint particles added. Copper accumulation by the alga increased linearly with aqueous Cu concentration, largely through adsorption to the cell surface, but significant accumulation of Zn was not observed. Thus, in coastal environments where boat maintenance is practiced, discarded antifouling paint particles are an important source of Cu, but not Zn, to U. lactuca. - The marine macroalga, Ulva lactuca, is able to accumulate Cu but not Zn from discarded antifouling paint particles.

95

Aspects of iron nutrition in macroalgae Ulva pertusa (Chlorophyta) under iron stress  

Science.gov (United States)

Fe, Chlorophyll (Chl) and total nitrogen (TN) content in tissues were measured in Fe-deficient cultures of Ulva. pertusa over a period of 60 days. Photosynthetic carbon fixation rates were studied at the start of and 30 days after Fe-deficiency culture, when the effects of Fe-deficiency on the ultrastructure were also analyzed. The iron content in tissue decreased exponentially during Fe-deficiency (from 726.7 to 31.6 ?g/gdw) and simultaneously Chl and TN content declined to 4.35% and 59.9% of their original levels respectively. Maximum carbon fixation rate (50 250 ?mol/m2 s) under Fe-deficiency decreased significantly compared with the control (pUlva. pertusa under Fe-deficiency could be due mainly to nitrogen utilization and inhibition of photosynthesis.

Liu, Jing-Wen; Dong, Shuang-Lin; Liu, Xiao-Yun

2002-06-01

96

Adsorption and thermodynamic behavior of uranium(VI) on Ulva sp.-Na bentonite composite adsorbent  

International Nuclear Information System (INIS)

The algae-clay composite adsorbent was tested for its ability to recover U(VI) from diluted aqueous solutions. Macro marine algae (Ulva sp.) and clay (Na bentonite) were used to prepare composite adsorbent. The ability of the composite adsorbent to adsorp uranium(VI) from aqueous solution has been studied at different optimized conditions of pH, concentration of U(VI), temperature, contact time. Parameters of desorption were also investigated to recover the adsorbed uranium. The adsorption patterns of uranium on the composite adsorbent followed the Freundlich and Dubinin-Radushkevich isotherms. The thermodynamic parameters such as the enthalpy ?H, entropy ?S and Gibbs free energy ?G were calculated from the slope and intercept of lnKd vs. 1/T plots. The results suggested that the Ulva sp.-Na bentonite composite adsorbent is suitable as sorbent material for recovery and biosorption/adsorption of uranium ions from aqueous solutions. (author)

97

Avaliação da ação da macroalga marinha Ulva lactuca em animais de experimentação  

Directory of Open Access Journals (Sweden)

Full Text Available Ulva lactuca é uma macroalga verde marinha comum em ambientes de temperatura elevada e com grandes variações de salinidade. A flora ficológica marinha vem sendo objeto de estudo por apresentar propriedades farmacoterapêuticas sobre o metabolismo animal, além de oferecer uma alternativa viável no tratamento de patologias que acometem o ser humano e afeta a sua qualidade de vida, ela é bastante utilizada na culinária, como iogurte, sushi. Objetivando avaliar a toxicidade aguda da Ulva lactuca foram utilizados camundongos albinos Swiss (Mus musculus, durante os estudos foi evidenciado que a alga tem efeitos estimulante do sistema nervoso central e periférico, e que em doses elevadas como 2000 a 3750 mg/kg/ip podem causar alterações na morfologia dos órgão comprometendo seu funcionamento,já que foram observado na analise macroscópica dos órgãos que os vasos do trato gastrointestinal estavam dilatados, os rins hipertrofiados,o fígado isquêmico , pulmões hemorrágicos,e a bexiga cheia.

Carla Andrea de Moura

2013-09-01

98

Interacting effects of Hydrobia ulvae bioturbation and microphytobenthos on the erodibility of mudflat sediments  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Microphytobenthos-macrofauna sediment interactions and their effects on sediment erodability were examined in laboratory experiments. Sediment beds were manipulated in a tidal mesocosm to produce diatom mats in exponential or in stationary phases of development after 6, 8 or 11 d of culture. These sediment beds were used in flume experiments to investigate the influence of bioturbation by the gastropod Hydrobia ulvae on both sediment and pigment resuspension as a function of the physiological...

Orvain, Francis; Sauriau, Pierre-guy; Sygut, A.; Joassard, Lucette; Le Hir, Pierre

2004-01-01

99

Production of acetone, butanol, and ethanol from biomass of the green seaweed Ulva lactuca  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Green seaweed Ulva lactuca harvested from the North Sea near Zeeland (The Netherlands) was characterized as feedstock for acetone, ethanol and ethanol fermentation. Solubilization of over 90% of sugars was achieved by hot-water treatment followed by hydrolysis using commercial cellulases. A hydrolysate was used for the production of acetone, butanol and ethanol (ABE) by Clostridium acetobutylicum and Clostridium beijerinckii. Hydrolysate-based media were fermentable without nutrient supplemen...

Wal, H.; Sperber, B. L. H. M.; Houweling-tan, G. B. N.; Bakker, R. R. C.; Brandenburg, W. A.; Lo?pez-contreras, A. M.

2013-01-01

100

Comparative Studies of the Pyrolytic and Kinetic Characteristics of Maize Straw and the Seaweed Ulva pertusa  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Seaweed has attracted considerable attention as a potential biofuel feedstock. The pyrolytic and kinetic characteristics of maize straw and the seaweed Ulva pertusa were studied and compared using heating rates of 10, 30 and 50°C min?1 under an inert atmosphere. The activation energy, and pre-exponential factors were calculated by the Flynn-Wall-Ozawa (FWO), Kissinger-Akahira-Sunose (KAS) and Popescu methods. The kinetic mechanism was deduced by the Popescu method. The results indicate tha...

Ye, Naihao; Li, Demao; Chen, Limei; Zhang, Xiaowen; Xu, Dong

2010-01-01

 
 
 
 
101

Physiological responses of Ulva pertusa and U. armoricana to copper exposure.  

Science.gov (United States)

A comparative study of copper (Cu) toxicity and tolerance in two species of Ulva from Korea, the native Ulva pertusa and alien Ulva armoricana, was conducted by examining the effects on growth, pigmentation, chlorophyll fluorescence, antioxidant capacity and nitrate reductase activity. Toxic effects of Cu were less expressed in U. armoricana than in U. pertusa. At lower concentrations (25-50 microgL(-1)), exposure to Cu did not affect thallus growth of U. armoricana, whilst growth was significantly reduced in U. pertusa. An increase in chlorophyll concentrations was observed in U. armoricana exposed up to 100 microgL(-1), whereas Cu caused a significant chlorophyll reduction in U. pertusa. Chlorophyll b was reduced to a lesser extent than chlorophyll a by higher Cu concentrations. In U. armoricana, the maximum efficiency of photosystem II, minimum fluorescence, maximum electron transport rate and non-photochemical quenching were unaffected by Cu except at the highest concentration tested. U. pertusa showed a significant decrease in those parameters at much lower Cu concentrations. It was notable that in this alga the maximum efficiency of photosystem II was reduced at higher Cu concentrations than relative electron transport rate. Elevated concentrations of Cu induced a strong activation of antioxidant activity in U. armoricana, whereas the generation of high levels of reactive oxygen species probably decreased the non-enzymatic antioxidant defense system in U. pertusa. An increase in the nitrate reductase activity of U. armoricana at 50-100 microgL(-1) Cu coincided with the increase in chlorophyll contents, whereas U. pertusa showed a significant decrease at the higher Cu concentration. Differences in the sensitivity of the two species of Ulva to Cu may influence their competitive interactions in Korean coastal waters experiencing temporal increases in the loading of heavy metals. PMID:18083244

Han, Taejun; Kang, Sung-Ho; Park, Ji-Sook; Lee, Han-Kyun; Brown, Murray T

2008-01-31

102

Eutrophication and macroalgal blooms in temperate and tropical coastal waters: nutrient enrichment experiments with Ulva spp.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Receiving coastal waters and estuaries are among the most nutrient-enriched environments on earth, and one of the symptoms of the resulting eutrophication is the proliferation of opportunistic, fast-growing marine seaweeds. Here, we used a widespread macroalga often involved in blooms, Ulva spp., to investigate how supply of nitrogen (N) and phosphorus (P), the two main potential growth-limiting nutrients, influence macroalgal growth in temperate and tropical coastal waters ranging from low- ...

Teichberg, Mirta; Fox, Sophia E.; Olsen, Ylva S.; Valiela, Ivan; Martinetto, Paulina; Iribarne, Oscar; Muto, Elizabeti Yuriko; Petti, Monica A. V.; Corbisier, Thai?s N.; Soto-jime?nez, Marti?n; Pa?ez-osuna, Federico; Castro, Paula; Freitas, Helena; Zitelli, Andreina; Cardinaletti, Massimo

2010-01-01

103

Sterol composition of the Adriatic Sea algae Ulva lactuca, Codium dichotomum, Cystoseira adriatica and Fucus virsoides  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The sterol composition of two green algae and two brown algae from the South Adriatic was determined. In the green alga Ulva lactuca, the principal sterols were cholesterol and isofucosterol. In the brown alga Cystoseira adriatica, the main sterols were cholesterol and stigmast-5-en-3ß-ol, while the characteristic sterol of the brown algae, fucosterol, was found only in low concentration. The sterol fractions of the green alga Codium dichotomum and the brown alga Fucus virsoides contained pr...

RADOMIR KAPETANOVIC; DUSAN SLADIC; SIMEON POPO; MARIO ZLATOVIC; ZORAN KLJAJIC; Gasic, Miroslav J.

2005-01-01

104

Physiological responses of Ulva pertusa and U. armoricana to copper exposure  

Energy Technology Data Exchange (ETDEWEB)

A comparative study of copper (Cu) toxicity and tolerance in two species of Ulva from Korea, the native Ulva pertusa and alien Ulva armoricana, was conducted by examining the effects on growth, pigmentation, chlorophyll fluorescence, antioxidant capacity and nitrate reductase activity. Toxic effects of Cu were less expressed in U. armoricana than in U. pertusa. At lower concentrations (25-50 {mu}g L{sup -1}), exposure to Cu did not affect thallus growth of U. armoricana, whilst growth was significantly reduced in U. pertusa. An increase in chlorophyll concentrations was observed in U. armoricana exposed up to 100 {mu}g L{sup -1}, whereas Cu caused a significant chlorophyll reduction in U. pertusa. Chlorophyll b was reduced to a lesser extent than chlorophyll a by higher Cu concentrations. In U. armoricana, the maximum efficiency of photosystem II, minimum fluorescence, maximum electron transport rate and non-photochemical quenching were unaffected by Cu except at the highest concentration tested. U. pertusa showed a significant decrease in those parameters at much lower Cu concentrations. It was notable that in this alga the maximum efficiency of photosystem II was reduced at higher Cu concentrations than relative electron transport rate. Elevated concentrations of Cu induced a strong activation of antioxidant activity in U. armoricana, whereas the generation of high levels of reactive oxygen species probably decreased the non-enzymatic antioxidant defense system in U. pertusa. An increase in the nitrate reductase activity of U. armoricana at 50-100 {mu}g L{sup -1} Cu coincided with the increase in chlorophyll contents, whereas U. pertusa showed a significant decrease at the higher Cu concentration. Differences in the sensitivity of the two species of Ulva to Cu may influence their competitive interactions in Korean coastal waters experiencing temporal increases in the loading of heavy metals.

Han, Taejun [Division of Biology and Chemistry, University of Incheon, Incheon 402-749 (Korea, Republic of)], E-mail: hanalgae@incheon.ac.kr; Kang, Sung-Ho [Korea Polar Research Institute, Incheon 406-840 (Korea, Republic of); Park, Ji-Sook; Lee, Han-Kyun [Division of Biology and Chemistry, University of Incheon, Incheon 402-749 (Korea, Republic of); Brown, Murray T. [School of Biological Sciences, University of Plymouth, Plymouth, Devon, PL4 8AA (United Kingdom)

2008-01-31

105

ANTIMICROBIAL ACTIVITY OF GREEN SEAWEED Ulva reticulata COLLECTED FROM TAKALAR WATERS OF SOUTH SULAWESI  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Introduction Seaweeds are the extraordinary sustainable resources in the marine ecosystem which have been used as a source of food, feed and medicine. These marine natural products possess a broad range of activity as antibacterial, antiviral, antifungal, cytotoxic and anticancer. Methods Study of antimicrobial activity and TLC-bioautography of green seaweed Ulva reticulata extracts against human pathogenic bacteria and yeast has been done invitro by agar diffusion met...

Zainuddin, Elmi N.; Manggau, Marianti A.; Sartini; Wan Nawi, W. N. F.

2014-01-01

106

Allelopathic Interactions between the Opportunistic Species Ulva prolifera and the Native Macroalga Gracilaria lichvoides  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Allelopathy, one type of direct plant competition, can be a potent mechanism through which plant communities are structured. The aim of this study was to determine whether allelopathic interactions occur between the opportunistic green tide-forming species Ulva prolifera and the native macroalga Gracilaria lichvoides, both of which were collected from the coastline of East China sea. In laboratory experiments, the presence of G. lichvoides at 1.25 g wet weight L?1 significantly inhibited gr...

Xu, Dong; Gao, Zhengquan; Zhang, Xiaowen; Fan, Xiao; Wang, Yitao; Li, Demao; Wang, Wei; Zhuang, Zhimeng; Ye, Naihao

2012-01-01

107

Physiological responses of Ulva pertusa and U. armoricana to copper exposure  

International Nuclear Information System (INIS)

A comparative study of copper (Cu) toxicity and tolerance in two species of Ulva from Korea, the native Ulva pertusa and alien Ulva armoricana, was conducted by examining the effects on growth, pigmentation, chlorophyll fluorescence, antioxidant capacity and nitrate reductase activity. Toxic effects of Cu were less expressed in U. armoricana than in U. pertusa. At lower concentrations (25-50 ?g L-1), exposure to Cu did not affect thallus growth of U. armoricana, whilst growth was significantly reduced in U. pertusa. An increase in chlorophyll concentrations was observed in U. armoricana exposed up to 100 ?g L-1, whereas Cu caused a significant chlorophyll reduction in U. pertusa. Chlorophyll b was reduced to a lesser extent than chlorophyll a by higher Cu concentrations. In U. armoricana, the maximum efficiency of photosystem II, minimum fluorescence, maximum electron transport rate and non-photochemical quenching were unaffected by Cu except at the highest concentration tested. U. pertusa showed a significant decrease in those parameters at much lower Cu concentrations. It was notable that in this alga the maximum efficiency of photosystem II was reduced at higher Cu concentrations than relative electron transport rate. Elevated concentrations of Cu induced a strong activation of antioxidant activity in U. armoricana, whereas the generation of high levels of reactive oxygen species probably decreased the non-enzymatic antioxidant defense syste non-enzymatic antioxidant defense system in U. pertusa. An increase in the nitrate reductase activity of U. armoricana at 50-100 ?g L-1 Cu coincided with the increase in chlorophyll contents, whereas U. pertusa showed a significant decrease at the higher Cu concentration. Differences in the sensitivity of the two species of Ulva to Cu may influence their competitive interactions in Korean coastal waters experiencing temporal increases in the loading of heavy metals

108

Comparison of the effectiveness of four organic chemoattractants towards zoospores of Ulva pertusa and macrofouling.  

Science.gov (United States)

Algal spores respond to many environmental variables, especially to chemical "cues". This chemotactic response can be utilized to attract spores, thereby colonization of a new substrata is possible to be influenced. In this attempt, four chemoattractant candidates were screened against spores of Ulva pertusa to reveal their efficiencies. Attachment and subsequent germination of Ulva spores were effectively influenced by these chemoattractant candidates. In particular 100 microg cm2 of D-glucose coating was found to enhance spore attachment by > 150%. Furthermore, field investigations carried out with test panels, clearly indicate the chemoattractive properties of test coatings. In recent years, various anthropogenic activities and natural hazards cause detrimental impacts on the benthic algae and other fishery resources. Artificial reefs have been laid on many coastal regions to increase or restore marine resources. Chemoattractant coatings can be applied on artificial surfaces to increase the colonization of benthic forms. It also can be used in the mariculture devices. Influence of chemoattractants on Ulva spores and fouling biomass estimated on test panels are discussed. PMID:19195407

Lee, Ji Hyun; Sidharthan, M; Jung, Sang Mok; Jo, Qtae; Rahman, Mohammad M; Shin, Hyun Woung

2008-07-01

109

Colonization potential of the genus Ulva (Chlorophyta, Ulvales in Comodoro Rivadavia Harbor (Chubut, Argentina  

Directory of Open Access Journals (Sweden)

Full Text Available Temporal and spatial changes in the coverage of five Ulva L. species (U. prolifera O.F. Müller, U. intestinalis L., U. hookeriana (Kützing Hayden et al., U. compressa L. and U. linza L. were analyzed on rocky substrata experimentally immersed in Comodoro Rivadavia Harbor (Argentina. Colonization was studied during an annual period at three levels: upper intertidal, middle intertidal and subtidal. Coverage was significantly higher in the middle intertidal and subtidal levels than in the upper intertidal level, where all species of Ulva were absent. Seasonal changes were also significant: coverage in summer and spring was significantly higher than in autumn and winter. Most species showed high spatial variability among replicates from the same level and season. Zonation patterns within the harbor differed from those observed in natural areas, probably due to low wave exposure and its interaction with herbivory and desiccation stress. Colonization patterns observed in this study indicate niche segregation in closely related species of the genus Ulva.

2005-01-01

110

Morphology and molecular identification of Ulva forming green tides in Qingdao, China  

Science.gov (United States)

Green tides are caused by the proliferation of chlorophytes under suitable hydrographic conditions. These blooms lead to environmental degradation and negatively impact the waters and seagrass beds, as well as fishing and other recreational activities in the bay. A comprehensive ecological understanding of the bloom dynamics, including the origin and persistence, is needed to foster management decisions. The algae in the great majority of green tide blooms usually belong to two genera of Ulvophyceae, Ulva and Enteromorpha. Ulva has been observed more often in recent years. In China, green tides occurred for the first time in the middle area of the Yellow Sea in 2007, and a large-scale algae blooming broke out in the middle and southern areas of the Yellow Sea in late May 2008. We identified them as Ulva prolifera by comparative analysis of the rDNA internal transcribed spacer 1 (ITS1), 5.8S and ITS2 sequences in combination with microscopic observation. Morphological differences were found between the free-floating algae and the attached thalli. Various reproduction patterns of the free-floating algae include sexual, asexual and vegetative propagations, which played important roles in the long-term green tide persistence in China. The ITS sequences of the blooming algae were identical to those of the samples from the Lianyungang sea area but were different from the attached samples from the Qingdao sea area. The results infer that the blooms are originated from other sea areas rather than from the local attached populations.

Kong, Fanna; Mao, Yunxiang; Cui, Fujun; Zhang, Xingkui; Gao, Zhen

2011-03-01

111

Protective and antigenotoxic effect of Ulva rigida C. Agardh in experimental hypothyroid.  

Science.gov (United States)

The presence of chromosomal damage in bone marrow cells affected by several diseases such as thyroid, cancer etc., was detected by the micronucleus (MN) assay. The present study was designed to evaluate: i) volatile components of Ulva rigida, ii) effects of hypothyroidism on bone marrow MN frequency, iii) effects of oral administration of Ulva rigida ethanolic extract (URE) on MN frequency produced by hypothyroidism, and iv) thyroid hormone levels in normal and 6-n-Propylthiouracil (PTU)-induced hypothyroid rats. The volatile components of Ulva rigida was studied using a direct thermal desorption (DTD) technique with comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GCxGC-TOF/MS). URE administration was of no significant impact on thyroid hormone levels in control group, while PTU administration decreased thyroid hormone levels compared to control group (p < 0.001). Moreover, URE supplementation resulted in a significant decrease in MN frequency in each thyroid group (p < 0.0001). This is the first in vivo study that shows the strong antigenotoxic and protective effect of URE against the genotoxicity produced by hypothyroidism. PMID:24561891

Celikler, Serap; Tas, Sibel; Ziyanok-Ayvalik, Sedef; Vatan, O; Yildiz, Gamze; Ozel, M

2014-03-01

112

Allometry of skull morphology, gape size and ingestion performance in the banded watersnake (Nerodia fasciata) feeding on two types of prey.  

Science.gov (United States)

Small body size imposes limitations on the feeding capabilities of juveniles, particularly in species that consume their prey whole. It has been hypothesized that juveniles exhibit exceptional performance measures to compensate for their small size. However, few studies have examined whether juveniles have better feeding performance relative to adults and investigations of snake feeding ontogeny have not shown enhanced performance in smaller snakes. I tested the hypothesis that juvenile snakes have better feeding performance by comparing maximum gape circumference and ingestion performance (time and number of pterygoid protractions) in a series of banded watersnakes (Nerodia fasciata) of different sizes fed fish and frogs. I also measured several external and osteological dimensions of the head and used Akaike's information criterion to determine which morphological measurements were the strongest predictors of relative gape. All skull measurements and maximum gape circumference showed negative allometry relative to snout-vent length (SVL). Given the available models, Akaike information criterion (AIC) analysis indicated that both skull length and mandible length were the strongest predictors of gape circumference for both external and osteological measurements. Multiple regression analysis of ingestion performance indicated SVL was negatively correlated with the time and number of pterygoid protractions required to consume fish or frogs, indicating that juveniles do not have a higher ingestion performance than adults. While exaggerated morphology in juvenile snakes does not appear to improve ingestion performance, a larger gape should increase the ability of juvenile snakes to consume a wide range of encountered prey shapes and sizes. PMID:24143025

Hampton, Paul M

2014-02-01

113

Establishment of caudal fin cell lines from tropical ornamental fishes Puntius fasciatus and Pristolepis fasciata endemic to the Western Ghats of India.  

Science.gov (United States)

Two new cell lines, PFF and CFF were established from the caudal fin of the Puntius fasciatus, and Pristolepis fasciata respectively. Since their initiation, these cell lines (PFF and CFF) have been subcultured in L-15 medium with 10% fetal bovine serum for more than 35 passages at 28°C and both the cell lines were characterized. Karyotyping analysis of PFF and CFF cells at 25th passage indicated that the modal chromosome number was 2n=50 and 2n=48 respectively. The cell line was cryopreserved in liquid nitrogen at -196°C and could be recovered from storage after six months with good cell viability. Polymerase chain reaction amplification of the fragments of two mitochondrial genes, 16S rRNA and COI confirmed that the cell lines originated from the respective species. The bacterial extracellular products from Vibrio cholerae MTCC3904 and Aeromonas hydrophila were found to be toxic to PFF and CFF. Both the cells were resistant to the marine viral nervous necrosis virus (VNNV). No CPE could be found in both cell lines inoculated with the fish samples and cell culture supernatants were demonstrated free of SVC, iridovirus and KHV by molecular methods. These results indicated the absence of SVC, iridovirus and KHV in the ornamental fishes collected from the Western Ghats of India. PMID:23933168

Swaminathan, T Raja; Basheer, V S; Gopalakrishnan, A; Rathore, Gaurav; Chaudhary, Dharmendra K; Kumar, Raj; Jena, J K

2013-12-01

114

[Marine inorganic carbon system responses to macro-DIN supply coupled with Ulva pertusa in simulated experiments].  

Science.gov (United States)

Effects of macronutrient (NO3(-) -N and NH4(+) -N) on inorganic carbon system of water with Ulva pertusa existed were studied in laboratory simulation experiments. The results demonstrated that nutrient enrichment induced DIC, HCO3- and p(CO2) decreased while pH and CO3(2-) increased. The seawater changed from carbon source to carbon sink. During the experiments, the concentration of DIC, HCO3- and p(CO2) decreased with increasing concentration of nutrient when the NO3(-) -N and N4(+) -N were less than critical concentration. The concentration of DIC changed most at the NO3(-3) and NH4(-)3 groups, which decreased 151 micromol x L(-1) and 232 micromol x L(-1) compared with the control groups in the end of experiment. The increased dry weight of Ulva pertusa (deltam) of nutrient addition groups showed a significant negative correlation with deltaDIC (r = - 0.91, p Ulva pertusa to different DIN. When the concentration of DIN facilitates the growth of Ulva pertusa, the concentration of DIC decreased and dry weight of Ulva pertusa increased. NH(4) -N has more influence on inorganic carbon system than NO(3-) -N. PMID:19774983

Zhang, Nai-Xing; Song, Jin-Ming; Guo, Ming-Ke; Cao, Cong-Hu; Ren, Rong-Zhu; Wu, Feng-Cong; Wang, Jin-Wen

2009-07-15

115

Inorganic nitrogen control in wastewater treatment ponds from a fish farm (Orbetello, Italy): Denitrification versus Ulva uptake  

International Nuclear Information System (INIS)

The aim of this study was to quantify the N removal efficiency of an Ulva-based phytotreatment system receiving wastewaters from a land-based fish farm (Orbetello, Italy), to identify the main biogeochemical pathways involved and to provide basic guidelines for treatment implementation and management. Fluxes of O2 and nutrients in bare and in Ulva colonised sediments were assessed by light/dark core incubations; denitrification by the isotope pairing technique and Ulva growth by in situ incubation of macroalgal disks in cages. O2 and nutrient budgets were estimated as sum of individual processes and further verified by 24-h investigations of overall inlet and outlet loads. Ulva uptake (up to 7.8mmolNm-2h-1) represented a net sink for water column and regenerated NH4+ whilst N removal via denitrification (10-170?molNm-2h-1) accounted for a small percentage of inorganic nitrogen load (-2h-1) indicating that N loss could be enhanced. The control of Ulva standing stocks by optimised harvesting of surplus biomass may represent an effective strategy to maximise DIN removal and could result in the assimilation of ?50% of produced inorganic nitrogen

116

Inorganic nitrogen control in wastewater treatment ponds from a fish farm (Orbetello, Italy): Denitrification versus Ulva uptake  

Energy Technology Data Exchange (ETDEWEB)

The aim of this study was to quantify the N removal efficiency of an Ulva-based phytotreatment system receiving wastewaters from a land-based fish farm (Orbetello, Italy), to identify the main biogeochemical pathways involved and to provide basic guidelines for treatment implementation and management. Fluxes of O{sub 2} and nutrients in bare and in Ulva colonised sediments were assessed by light/dark core incubations; denitrification by the isotope pairing technique and Ulva growth by in situ incubation of macroalgal disks in cages. O{sub 2} and nutrient budgets were estimated as sum of individual processes and further verified by 24-h investigations of overall inlet and outlet loads. Ulva uptake (up to 7.8mmolNm{sup -2}h{sup -1}) represented a net sink for water column and regenerated NH{sub 4}{sup +} whilst N removal via denitrification (10-170{mu}molNm{sup -2}h{sup -1}) accounted for a small percentage of inorganic nitrogen load (<5%). Laboratory experiments demonstrated a high potential for denitrification (over 800{mu}MNm{sup -2}h{sup -1}) indicating that N loss could be enhanced. The control of Ulva standing stocks by optimised harvesting of surplus biomass may represent an effective strategy to maximise DIN removal and could result in the assimilation of {approx}50% of produced inorganic nitrogen.

Bartoli, Marco [DSA, Universita di Parma, 43100 PR (Italy)]. E-mail: bartoli@dsa.unipr.it; Nizzoli, Daniele [DSA, Universita di Parma, 43100 PR (Italy); Naldi, Mariachiara [DSA, Universita di Parma, 43100 PR (Italy); Vezzulli, Luigi [DIPTERIS, Universita di Genova, 16142 GE (Italy); Porrello, Salvatore [ICRAM, 300-00166 Rome (Italy); Lenzi, Mauro [LEALab, OPL s.r.l., 58015 Orbetello, GR (Italy); Viaroli, Pierluigi [DSA, Universita di Parma, 43100 PR (Italy)

2005-11-15

117

Influence of five organic antifouling candidates on spore attachment and germination of a fouling alga Ulva pertusa.  

Science.gov (United States)

Screening of test chemicals or formulations for antifouling (AF) activity is important to get first hand information on their nontoxic repelling activities. Especially spores of a fouling alga, Ulva pertusa were used in this study to test the AF efficiency of five organic chemicals. Coatings made with 100 microg cm2 of citral and eugenol significantly inhibited the spore attachment. A low concentration (1 microg cm2) of solanesol exhibited effective AF activity against spore attachment. Spore germination was sensitive to different AF candidates screened in this study. Based on the attachment and germination response of Ulva pertusa spores, AF efficiency of five organic AF candidates is discussed. PMID:17717983

Sidharthan, M; Shin, H W

2007-01-01

118

A NEW LOCALITY OF THE FRESHWATER POPULATION OF Ulva flexuosa subsp. pilifera (Chlorophyta, Ulvophyceae) IN POZNA? (WIELKOPOLSKA)  

Digital Repository Infrastructure Vision for European Research (DRIVER)

In the latter part of the April of 2011 green algae thalli from the genus Ulva were found at the new, until now not known site in the Wielkopolska region. Thalli of Ulva (syn. Enteromorpha) flexuosa subsp. pilifera (Kützing) Bliding 1963 were collected to a further morphological research from the small, nameless, artificial dam reservoir located in the north part of Pozna?. It was the second site, after the Malta Lake, of its taxon in Pozna? and the sixth site in Poland. At the newly desc...

Rybak, Andrzej; Messyasz, Beata; Szendzina, Lidia; Pikosz, Marta; Koperski, Maciej

2011-01-01

119

Allelopathic interactions between the macroalga Ulva pertusa and eight microalgal species  

Science.gov (United States)

Growth of Ulva pertusa and eight microalgal species, Heterosigma akashiwo, Skeletonema costatum, Tetraselmis subcordiformis, Nitzschia closterium, Chaetoceros gracile, Chroomonas placoidea 1967, Isochrysis galbana 8701, and Alexandrium tamarense, was examined in a series of batch, semi-continuous and isolated co-cultures ( U. pertusa and one microalgal species). The results of the experiments with co-cultures confirmed the secretion of allelopathic substances by U. pertusa. Growth was significantly ( pclosterium (-23%) and C. gracile (-30%), but was not significantly affected by I. galbana, A. tamarense and C. placoidea. The microalgae tested exhibited no clear allelopathic effects on U. pertusa.

Nan, Chunrong; Zhang, Haizhi; Zhao, Guangqiang

2004-11-01

120

Requisite Morphologic Interaction for Attachment between Ulva pertusa (Chlorophyta) and Symbiotic Bacteria.  

Science.gov (United States)

: In order to understand the morphogenesis-inducing mechanism of Ulva pertusa by symbiotic bacteria, we observed the requisite conditions of bacteria for attachment to U. pertusa for algal morphogenesis. Non-morphogenesis-inducing bacterial mutants derived by ultraviolet irradiation did not attach onto the surface of this alga. Scanning electron microscopic observation during the process of morphogenesis in U. pertusa revealed a network-like structure formed on the algal surface within 1 week after application of bacteria. The bacteria attached onto the alga after 2 weeks of incubation. After this attachment process the morphologic change was observed in U. pertusa. PMID:10373618

Nakanishi; Nishijima; Nomoto; Yamazaki; Saga

1999-01-01

 
 
 
 
121

Effect of extraction conditions on the yied and purity of ulvan extracted from Ulva lactuca  

Digital Repository Infrastructure Vision for European Research (DRIVER)

A study of the influence of extraction conditions (pH: 1.5 and 2; temperature: 80 °C and 90 °C; extraction time: 1-3 h), on the yield, chemical composition and purity of the sulphated cell wall polysaccharides ulvan, extracted from the green seaweed Ulva lactuca and precipitated by alcohol is carried out. The alcohol precipitate yields varied from 21.68% to 32.67% (%dw/dw) depending on the pH. At pH 2, the alcohol precipitate yields and the uronic acid recovery from extract juice are higher...

Yaich, Hela; Garna, Haikel; Besbes, Souhail; Paquot, Michel; Blecker, Christophe; Attia, Hamadi

2013-01-01

122

Adsorption characteristics of the heavy metals, Cd(II) and Pb(II) ions, on the Si-immobilized ulva pertusa  

Energy Technology Data Exchange (ETDEWEB)

Adsorption characteristics of heavy metal ions, Cd(II) and Pb(II), on eastcoast-living algae, ulva pertusa, has been studied in our experiment. The maximum adsorption amount of Cd(II) and Pb(II) ions on 1 g of the ulva pertusa were 2.3 mg, 3.1 mg in alkaline and 2.0 mg, 2.8 mg in acidic solution. However 3.4 mg, 7.3 mg in alkaline and 3.1 mg, 5.5 mg in acidic solution were shown on the Si-immobilized ulva pertusa in the same condition .Thus, Si-immobilized ulva pertusa adsorbs more amount of heavy metals, Cd(II), Pb(II), than the ulva pertusa, and more effective absorbent in alkaline. Furthermore, more amounts of Pb(II) ion were absorbed compare to Cd(II) ion in our work. Recovery ratio of Cd(II) and Pb(II) ions on the ulva pertusa were 55.0{approx}61.0%, 59.7{approx}66.8% respectively and 87.6{approx}97.5%, 83.5{approx}99.3% on the Si-immobilized ulva pertusa. 28 refs., 10 figs., 4 tabs.

Park, K.H.; Park, M.A.; Kim, Y.H. [Kangnung National University, Kangnung (Korea, Republic of); Choi, B.J. [Kwan Dong University, Yang Yang (Korea, Republic of)

1999-04-01

123

Exposure of the marine deposit feeder Hydrobia ulvae to sediment associated LAS  

International Nuclear Information System (INIS)

Linear Alkylbenzene Sulfonates (LAS) effects (mortality, egestion rate, behaviour) on the marine deposit feeder Hydrobia ulvae were assessed in whole-sediment and water-only systems. The results were combined with a bioenergetic-based kinetic model of exposure pathways to account for the observed toxicity. The 10-d LC50 value based on the freely dissolved fraction was 9.3 times lower in spiked sediment (0.152 ± 0.001 (95% CI) mg/L) than in water-only (1.390 ± 0.020 (95% CI) mg/L). Consequently, the actual 10-d LC50 value (208 mg/kg) was overestimated by the Equilibrium Partitioning calculation (1629 mg/kg). This suggests that the sediment associated LAS fraction was bioavailable to the snails. It could also be due to modifications in physiological parameters in absence of sediment, the organism natural substrate. - Lethality of the marine gastropod deposit feeder Hydrobia ulvae exposed to LAS in water-only system was inappropriate to predict LAS toxicity in sediment system.

124

Molecular characterization of a new lectin from the marine alga Ulva pertusa.  

Science.gov (United States)

A new lectin, named UPL1, was purified from a green alga Ulva pertusa by an affinity chromatography on the bovine-thyroglobulin-Sepharose 4B column. The molecular mass of the algal lectin was about 23 kD by SDS-PAGE, and it specifically agglutinated rabbit erythrocytes. The hemagglutinating activity for rabbit erythrocytes could be inhibited by bovine thyroglobulin and N-acetyl-D-glucosamine. The lectin UPL1 required divalent cations for maintenance of its biological activity, and was heat-stable, and had higher activity within pH 6-8. The N-terminal amino acid sequence of the purified lectin was determined (P83209) and a set of degenerate primers were designed. The full-length cDNA of the lectin was cloned by rapid amplification of cDNA ends (RACE) method (AY433960). Sequence analysis of upl1 indicated it was 1084 bp long, and encoded a premature protein of 203 amino acids. The N-terminal sequence of the mature UPL1 polypeptide started at amino acid 54 of the deduced sequence from the cDNA, indicating 53 amino acids lost due to posttranslational modification. The primary structure of the Ulva pertusa lectin did not show amino acid sequence similarity with known plant and animal lectins. Hence, this protein may be the paradigm of a novel lectin family. PMID:14970906

Wang, Sheng; Zhong, Fu-Di; Zhang, Yong-Jiang; Wu, Zu-Jian; Lin, Qi-Ying; Xie, Lian-Hui

2004-02-01

125

Exposure of the marine deposit feeder Hydrobia ulvae to sediment associated LAS  

Energy Technology Data Exchange (ETDEWEB)

Linear Alkylbenzene Sulfonates (LAS) effects (mortality, egestion rate, behaviour) on the marine deposit feeder Hydrobia ulvae were assessed in whole-sediment and water-only systems. The results were combined with a bioenergetic-based kinetic model of exposure pathways to account for the observed toxicity. The 10-d LC50 value based on the freely dissolved fraction was 9.3 times lower in spiked sediment (0.152 +- 0.001 (95% CI) mg/L) than in water-only (1.390 +- 0.020 (95% CI) mg/L). Consequently, the actual 10-d LC50 value (208 mg/kg) was overestimated by the Equilibrium Partitioning calculation (1629 mg/kg). This suggests that the sediment associated LAS fraction was bioavailable to the snails. It could also be due to modifications in physiological parameters in absence of sediment, the organism natural substrate. - Lethality of the marine gastropod deposit feeder Hydrobia ulvae exposed to LAS in water-only system was inappropriate to predict LAS toxicity in sediment system.

Mauffret, A., E-mail: aourell.mauffret@icman.csic.e [Marine Sciences Institute of Andalusia (CSIC), Puerto Real (Cadiz) (Spain); Rico-Rico, A. [Institute for Risk Assessment Sciences, Utrecht University (Netherlands); Temara, A. [The Procter and Gamble Company, Brussels (Belgium); Blasco, J. [Marine Sciences Institute of Andalusia (CSIC), Puerto Real (Cadiz) (Spain)

2010-02-15

126

Toxicity of tire wear particle leachate to the marine macroalga, Ulva lactuca  

Energy Technology Data Exchange (ETDEWEB)

Tire wear particles filed from the treads of end-of-life vehicle tires have been added to sea water to examine the release of Zn and the toxicity of the resulting leachate and dilutions thereof to the marine macroalga, Ulva lactuca. Zinc release appeared to be diffusion-controlled, with a conditional rate constant of 5.4 {mu}g[L(h){sup 1/2}]{sup -1}, and about 1.6% of total Zn was released after 120 h incubation. Exposure to increasing concentrations of leachate resulted in a non-linear reduction in the efficiency of photochemical energy conversion of U. lactuca and, with the exception of the undiluted leachate, increasing accumulation of Zn. Phototoxicity was significantly lower on exposure to equivalent concentrations of Zn added as Zn(NO{sub 3}){sub 2}, suggesting that organic components of leachate are largely responsible for the overall toxicity to the alga. Given the ubiquity and abundance of TWP in urban coastal sediments, the generation, biogeochemistry and toxicity of tire leachate in the marine setting merit further attention. - Tire wear leachate is toxic to Ulva lactuca and zinc is a potential bioindicator of leachate contamination in urban marine systems.

Turner, Andrew, E-mail: aturner@plymouth.ac.u [School of Geography, Earth and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Rice, Lynsey [School of Geography, Earth and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom)

2010-12-15

127

Bioremediation of reject water from anaerobically digested waste water sludge with macroalgae (Ulva lactuca, Chlorophyta).  

Science.gov (United States)

Phosphorus and biologically active nitrogen are valuable nutrient resources. Bioremediation with macroalgae is a potential means for recovering nutrients from waste streams. In this study, reject water from anaerobically digested sewage sludge was successfully tested as nutrient source for cultivation of the green macroalgae Ulva lactuca. Maximal growth rates of 54.57±2.16% FW d(-1) were achieved at reject water concentrations equivalent to 50 ?M NH4(+). Based on the results, the growth and nutrient removal was parameterised as function of NH4(+) concentration a tool for optimisation of any similar phycoremediation system. Maximal nutrient removal rates of 22.7 mg N g DW(-1) d(-1) and 2.7 mg P g DW(-1) d(-1) were achieved at reject water concentrations equivalent to 80 and 89 ?M NH4(+), respectively. A combined and integrated use of the produced biomass in a biorefinery is thought to improve the feasibility of using Ulva for bioremediation of reject water. PMID:23954716

Sode, Sidsel; Bruhn, Annette; Balsby, Thorsten J S; Larsen, Martin Mørk; Gotfredsen, Annemarie; Rasmussen, Michael Bo

2013-10-01

128

Toxicity of tire wear particle leachate to the marine macroalga, Ulva lactuca  

International Nuclear Information System (INIS)

Tire wear particles filed from the treads of end-of-life vehicle tires have been added to sea water to examine the release of Zn and the toxicity of the resulting leachate and dilutions thereof to the marine macroalga, Ulva lactuca. Zinc release appeared to be diffusion-controlled, with a conditional rate constant of 5.4 ?g[L(h)1/2]-1, and about 1.6% of total Zn was released after 120 h incubation. Exposure to increasing concentrations of leachate resulted in a non-linear reduction in the efficiency of photochemical energy conversion of U. lactuca and, with the exception of the undiluted leachate, increasing accumulation of Zn. Phototoxicity was significantly lower on exposure to equivalent concentrations of Zn added as Zn(NO3)2, suggesting that organic components of leachate are largely responsible for the overall toxicity to the alga. Given the ubiquity and abundance of TWP in urban coastal sediments, the generation, biogeochemistry and toxicity of tire leachate in the marine setting merit further attention. - Tire wear leachate is toxic to Ulva lactuca and zinc is a potential bioindicator of leachate contamination in urban marine systems.

129

The nucleotide sequences of 5S rRNAs from a multicellular green alga, Ulva pertusa, and two brown algae, Eisenia bicyclis and Sargassum fulvellum.  

Science.gov (United States)

The nucleotide sequences of 5S rRNA from a multicellular green alga Ulva pertusa, and multicellular brown algae Eisenia bicyclis and Sargassum fulvellum, have been determined. The 5S rRNA from Ulva is composed of 120 nucleotides, and those from Eisenia and Sargassum have 118 nucleotides. The nucleotide sequence of Ulva 5S rRNA is rather similar to 5S rRNAs from unicellular green algae and higher plants, while those of Eisenia and Sargassum 5S rRNAs are unique. PMID:6835842

Lim, B L; Hori, H; Osawa, S

1983-03-25

130

The nucleotide sequences of 5S rRNAs from a multicellular green alga, Ulva pertusa, and two brown algae, Eisenia bicyclis and Sargassum fulvellum.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The nucleotide sequences of 5S rRNA from a multicellular green alga Ulva pertusa, and multicellular brown algae Eisenia bicyclis and Sargassum fulvellum, have been determined. The 5S rRNA from Ulva is composed of 120 nucleotides, and those from Eisenia and Sargassum have 118 nucleotides. The nucleotide sequence of Ulva 5S rRNA is rather similar to 5S rRNAs from unicellular green algae and higher plants, while those of Eisenia and Sargassum 5S rRNAs are unique.

Lim, B. L.; Hori, H.; Osawa, S.

1983-01-01

131

Alpha-oxidation of long-chain unsaturated fatty acids in the marine green alga Ulva pertusa.  

Science.gov (United States)

When long-chain unsaturated fatty acids such as oleic, linoleic, and linolenic acid were incubated with crude enzymes from the marine green alga Ulva pertusa, the corresponding (R)-2-hydroperoxy acids were formed with a high enantiomeric excess (>99%). PMID:11210134

Akakabe, Y; Matsui, K; Kajiwara, T

2000-12-01

132

DEVELOPMENTAL CHANGES IN THE GERMINATION, GROWTH AND CHLOROPHYLLASE ACTIVITY OF VIGNA MUNGO L. USING SEAWEED EXTRACT OF ULVA RETICULATA FORSSKAL.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The effect of seaweed extract prepared from Ulva reticulata on seed germination, seedling growth and chlorophyllase activity of Vigna mungo L. was studied. 100% germination was recorded in the seeds treated with lower concentration of seaweed extract. The V. mungo seeds soaked with lower concentrations of the seaweed extracts showed higher rates of germination, while the higher concentrations of the extracts inhibited the germination.

Ganapathy Selvam G.; Balamurugan M; Thinakaran T.; Sivakumar K

2013-01-01

133

Influence of environment factors on bacterial ingestion rate of the deposit-feeder Hydrobia ulvae and comparison with meiofauna  

Science.gov (United States)

Deposit feeders are able to process a considerable volume of sediment, containing large quantities of associated bacteria. However, conclusions concerning the trophic role played by benthic bacteria in marine sediments are still not fully elucidated. This study deals with bacterivory by the gastropod Hydrobia ulvae, one of the most abundant deposit-feeding species in intertidal mudflats in Western Europe. Ingestion rates of bacteria were determined during grazing experiments using 15N pre-enriched bacteria. Grazing experiments were performed in order to measure effects of abiotic (temperature, salinity and luminosity) and biotic (bacterial and algal abundances) factors on ingestion rates of bacteria by H. ulvae of an intertidal mudflat (Brouage, Marennes-Oléron, France). The mean ingestion rate of bacteria by H. ulvae was 1149 ng C ind - 1 h - 1 . The general trend showed a temperature effect with an optimum around 30 °C, and the assimilation rate was significantly lower at 5 °C. Bacterial assimilation did not significantly differ between salinity 18 and salinity 31. Ingestion was the same in light and in dark conditions. Results were compared with those of other grazing experiments conducted simultaneously in similar conditions with two other grazers with different size and feeding modes: the foraminifera Ammonia tepida and a nematode community from the superficial sediment of the Brouage mudflat. H. ulvae and nematodes presented a feeding behavior less influenced by environmental changes than A. tepida. H. ulvae ingested bacteria at a higher rate than smaller meiofaunal grazers and seemed to have a lower ability to selectively ingest diatoms than meiofaunal grazers.

Pascal, Pierre-Yves; Dupuy, Christine; Richard, Pierre; Haubois, Anne-Gaelle; Niquil, Nathalie

2008-10-01

134

INFLUÊNCIA DO CANAL MARINHO DA ILHA DE VITÓRIA-ES SOBRE A BIOMASSA DE ALGAS DO GÊNERO Ulva sp. (CHLOROPHYTA, ULVALES)  

Digital Repository Infrastructure Vision for European Research (DRIVER)

As informações a respeito do tamanho da biomassa de Ulva sp aqui apresentadas têm por objetivo contribuirpara o conhecimento deste gênero, focando o fornecimento de subsídios para estratégias de conservação doecossistema aquático. O objetivo deste estudo foi observar se ocorre diferença na biomassa de Ulva sp emdiferentes pontos localizados próximos a um canal com poluição orgânica de esgoto. As condições doambiente ou fatores abióticos, como a poluição, induzem uma serie d...

Vitor Araújo-Lima; Nuno Gouvea de Souza Caliman

2012-01-01

135

Bioaccumulation of alkaline soil metals (Ca, Mg) and heavy metals (Cd, Ni, Pb) patterns expressed by freshwater species of Ulva (Wielkopolska, Poland)  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The paper includes results of the first study on the accumulation of selected metals in freshwater populations of Ulva taxa at 16 sites in Poland. The thalli examined contained very high concentrations of Ca and Mg, owing to well-developed surface incrustations of carbonate. Among the heavy metals investigated, the most significant concentration in the thalli was Ni, whereas the lowest was Pb. The median concentrations of Ni and Cd in the freshwater Ulva thalli were significantly ...

Rybak, Andrzej; Messyasz, Beata; ?e?ska, Bogus?awa

2012-01-01

136

Ulva flexuosa subsp. pilifera (Chlorophyta, Ulvophyceae) from the Wielkopolska region (West Poland): a new observation of the ultrastructure of vegetative cells  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Ulva flexuosa subsp. pilifera previously known from northern Poland, from the channel near Szczecin and ponds near ?ód?, has recently been found in the Malta Reservoir in the Wielkopolska (West Poland) region. Specimens collected in the Wielkopolska region were examined in detail, also under a transmission electron microscope (TEM). The morphometric analysis of Ulva thalli (both young and mature specimens) was performed in order to study the differences in the ultrastructure of vegetative ...

Messyasz, Beata; Czerwik-marcinkowska, Joanna; Uher, Bohuslav; Rybak, Andrzej; Szendzina, Lidia; Pikosz, Marta

2013-01-01

137

Freshwater Ulva (Chlorophyta) as a bioaccumulator of selected heavy metals (Cd, Ni and Pb) and alkaline earth metals (Ca and Mg)  

Digital Repository Infrastructure Vision for European Research (DRIVER)

We analyzed the ability of freshwater taxa of the genus Ulva (Ulvaceae, Chlorophyta) to serve as bioindicators of metal in lakes and rivers. Changes in heavy metal (Ni, Cd and Pb) and alkaline earth metal (Ca and Mg) concentrations in freshwater Ulva thalli were investigated during the period from June to August 2010. The study was conducted in two ecosystems in Western Poland, the Malta lake (10 sites) and the Nielba river (six sites). Three components were collected for each sample, includi...

Rybak, Andrzej; Messyasz, Beata; ?e?ska, Bogus?awa

2012-01-01

138

Effects of sediment type and intra- and interspecific competition on growth rate of the marine snails Hydrobia ulvae and Hydrobia ventrosa.  

Science.gov (United States)

The combined effects of sediment grain size and intra- and interspecific competition on growth rate of Hydrobia ulvae and H. ventrosa were investigated in a 2-month laboratory experiment. Individuals of H. ulvae grew faster than H. ventrosa at all competition intensities. Growth rate did not differ between fine- and coarse-grained sediments in any of the species. This suggests that other factors than the sediment quality cause the often observed size differences between Hydrobia snails from these sediment types in nature. Competition from 1.6 to 1.9 times larger competitors affected growth of the smaller target snails. Competition between the species was asymmetrical and, in both species, per capita effect of competition was stronger from H. ulvae than from H. ventrosa, regardless of sediment type. From growth, Lotka-Volterra competition coefficients were estimated to 0.2 from H. ventrosa on H. ulvae and 1.8 from H. ulvae on H. ventrosa. Since competition between the species seems to be independent of sediment grain size, the shift in numerical dominance in nature, from H. ulvae on coarse-grained sediment to H. ventrosa on fine-grained, could not be explained with different competitive ability in different environments. PMID:11018240

Grudemo; Bohlin

2000-10-01

139

Bioenergy potential of Ulva lactuca: Biomass yield, methane production and combustion  

DEFF Research Database (Denmark)

The biomass production potential at temperate latitudes (56°N), and the quality of the biomass for energy production (anaerobic digestion to methane and direct combustion) were investigated for the green macroalgae, Ulva lactuca. The algae were cultivated in a land based facility demonstrating a production potential of 45T (TS) ha(-1)y(-1). Biogas production from fresh and macerated U. lactuca yielded up to 271ml CH(4) g(-1) VS, which is in the range of the methane production from cattle manure and land based energy crops, such as grass-clover. Drying of the biomass resulted in a 5-9-fold increase in weight specific methane production compared to wet biomass. Ash and alkali contents are the main challenges in the use of U. lactuca for direct combustion. Application of a bio-refinery concept could increase the economical value of the U. lactuca biomass as well as improve its suitability for production of bioenergy.

Bruhn, Annette; Dahl, Jonas

2011-01-01

140

Bioenergy potential of Ulva lactuca: Biomass yield, methane production and combustion  

DEFF Research Database (Denmark)

The biomass production potential at temperate latitudes (56°N), and the quality of the biomass for energy production (anaerobic digestion to methane and direct combustion) were investigated for the green macroalgae, Ulva lactuca. The algae were cultivated in a land based facility demonstrating a production potential of 45 T (TS) ha?1 y?1. Biogas production from fresh and macerated U. lactuca yielded up to 271 ml CH4 g?1 VS, which is in the range of the methane production from cattle manure and land based energy crops, such as grass-clover. Drying of the biomass resulted in a 5–9-fold increase in weight specific methane production compared to wet biomass. Ash and alkali contents are the main challenges in the use of U. lactuca for direct combustion. Application of a bio-refinery concept could increase the economical value of the U. lactuca biomass as well as improve its suitability for production of bioenergy.

Bruhn, Annette; Dahl, Jonas

2011-01-01

 
 
 
 
141

Kinetics of inactivation of Ulva pertusa Kjellm alkaline phosphatase by ethylenediaminetetraacetic acid disodium.  

Science.gov (United States)

Ulva pertusa Kjellm alkaline phosphatase (EC 3.3.3.1) is a metalloenzyme, the active site of which contains a tight cluster of two zinc ions and one magnesium ion. The kinetic theory described by Tsou of the substrate reaction during irreversible inhibition of enzyme activity has been employed to study the kinetics of the course of inactivation of the enzyme by EDTA. The kinetics of the substrate reaction at different concentrations of the substrate p-nitrophenyl phosphate (PNPP) and inactivator EDTA indicated a complexing mechanism for inactivation by, and substrate competition with, EDTA at the active site. The inactivation kinetics are single phasic, showing that the initial formation of an enzyme-EDTA complex is a relative rapid reaction, following by a slow inactivation step that probably involves a conformational change of the enzyme. The presence of Zn2+ apparently stabilizes an active-site conformation required for enzyme activity. PMID:11916136

Yang, D; Wang, J; Peng, X; An, L

2001-10-01

142

Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitro.  

Science.gov (United States)

Polysaccharide extracted from Ulva pertusa (Chlorophyta) is a group of sulfated heteropolysaccharide; for simplicity, the sulfated polysaccharide is referred to as ulvan in this paper. In this study, different sulfate content ulvans were prepared with sulfur trioxide/N,N-dimethylformamide (SO3-DMF) in formamide, and their antioxidant activities were investigated including scavenging activity of superoxide and hydroxyl radicals, reducing power and metal chelating ability. As expected, we obtained several satisfying results, as follows: firstly, high sulfate content ulvans had more effective scavenging activity on hydroxyl radical than natural ulvan. Secondly, comparing with natural ulvan, high sulfate content ulvans exhibited stronger reducing power. Thirdly, HU4 (sulfate content, 30.8%) and HU5 (sulfate content, 32.8%) showed more pronounce chelating ability on ferrous ion at high concentration than other samples. PMID:16310843

Qi, Huimin; Zhang, Quanbin; Zhao, Tingting; Chen, Rong; Zhang, Hong; Niu, Xizhen; Li, Zhien

2005-12-15

143

Cardiac inhibitory action of constituents of the marine green alga Ulva pertusa.  

Science.gov (United States)

As part of a search for substances from marine organisms, which exhibit inotropic effects, the chemical constituents of the marine green alga, Ulva pertusa were investigated. The fractionated extract was tested for inotropic effects on the isolated guinea pig atria. The aqueous layer obtained from the acetone extract of fresh algae was concentrated, and the residue was extracted with methanol. The methanolic extract was fractionated by chromatography using mixtures of aqueous methanol. Elution with 50% aqueous methanol afforded material that had a significant negative inotropic effect. Further purification of this material by high-performance liquid chromatography (HPLC) using methanol-water (2:5) afforded crystalline adenosine, which was shown to be the active substance of U. pertusa, causing a negative inotropic action. PMID:6620154

Yamada, K; Shizuri, Y; Ishida, Y; Shibata, S

1983-08-01

144

Extreme irgarol tolerance in an Ulva lactuca L. population on the Swedish west coast.  

Science.gov (United States)

The herbicide irgarol 1051 is commonly used on ship hulls to prevent growth of algae, but as a component of self-eroding paints it can also spread in the surrounding waters and affect non-target organisms. The effect of irgarol on settlement and growth of zoospores from the marine macro algae Ulva lactuca from the Gullmar fjord on the Swedish west coast was investigated in the present study. The zoospores were allowed to settle and grow in the presence of irgarol, but neither settlement - nor growth inhibition was observed at concentrations of up to 2000 nmol l(-1). This is between 10 and 100 times higher than effect concentrations reported earlier for algae. Irgarol also induced the greening effect (4-fold increase in chlorophyll a content) in the settled zoospore/germling population, typical for photosystem II inhibitors like irgarol. This study support previous findings that irgarol constitutes a selection pressure in the marine environment. PMID:24054733

Wendt, Ida; Arrhenius, Åsa; Backhaus, Thomas; Hilvarsson, Annelie; Holm, Kristina; Langford, Katherine; Tunovic, Timur; Blanck, Hans

2013-11-15

145

Subchronic toxicity study of ulvan from Ulva pertusa (Chlorophyta) in Wistar rats.  

Science.gov (United States)

Ulvan extracted from Ulva pertusa (Chlorophyta) is a group of sulfated heteropolysaccharide, for simplicity, the sulfated polysaccharide is referred to as ulvan in this paper. To our knowledge, there is no detailed report investigating the toxicity of ulvan. In this study, the subchronic (6 months) toxicity of varying levels of ulvan extracted from U. pertusa was investigated in Wistar rats after oral administration. ALT, ALB, ALP, WBC, PLT, and liver relative organ weigh of female rats showed significantly difference at 3000 mg/kg body weight per day, compared with control group. On the other hand, TG, T-CHO concentrations of female rats (6 months) were significantly decreased at 600, 1200 and 3000 mg/kg body weight per day. This result proved that ulvan had antihyperlipidemic activity. Beside, ulvan showed anticoagulant activity in this study. Overall, our findings indicated that ulvan had affected specific hematology, serum biochemistry parameters and liver, and had great differences between males and females rats. PMID:24084035

Qi, Huimin; Liu, Xiaolei; Wang, Kai; Liu, Dongmei; Huang, Liye; Liu, Shunmei; Zhang, Quanbin

2013-12-01

146

Odmiany polimorficzne w?glanu wapnia na powierzchni plech Ulva (Chlorophyta) notowanych w wodach s?odkich  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Process of calcification, which is the formation of calcite — a pure calcium carbonate mineral, commonly takes place among both marine and freshwater algae. The available literature referring to inland waters provides a lot of information about calcareous macroalgae, mainly on extensively encrusted species of Chara, called stoneworts. In addition to this group, where CaCO3 may provide 60% of dry weight, also freshwater green alga Ulva is prone to CaCO3 precipitati...

Messyasz, Beata; Pikosz, Marta; Rybak, Andrzej

2014-01-01

147

Culture and Hybridization Experiments on an Ulva Clade Including the Qingdao Strain Blooming in the Yellow Sea  

Digital Repository Infrastructure Vision for European Research (DRIVER)

In the summer of 2008, immediately prior to the Beijing Olympics, a massive green tide of the genus Ulva covered the Qingdao coast of the Yellow Sea in China. Based on molecular analyses using the nuclear encoded rDNA internal transcribed spacer (ITS) region, the Qingdao strains dominating the green tide were reported to be included in a single phylogenetic clade, currently regarded as a single species. On the other hand, our detailed phylogenetic analyses of the clade, using a higher resolut...

Hiraoka, Masanori; Ichihara, Kensuke; Zhu, Wenrong; Ma, Jiahai; Shimada, Satoshi

2011-01-01

148

DEVELOPMENTAL CHANGES IN THE GERMINATION, GROWTH AND CHLOROPHYLLASE ACTIVITY OF VIGNA MUNGO L. USING SEAWEED EXTRACT OF ULVA RETICULATA FORSSKAL.  

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Full Text Available The effect of seaweed extract prepared from Ulva reticulata on seed germination, seedling growth and chlorophyllase activity of Vigna mungo L. was studied. 100% germination was recorded in the seeds treated with lower concentration of seaweed extract. The V. mungo seeds soaked with lower concentrations of the seaweed extracts showed higher rates of germination, while the higher concentrations of the extracts inhibited the germination.

Ganapathy Selvam G.

2013-01-01

149

The influence of surface lubricity on the adhesion of Navicula perminuta and Ulva linza to alkanethiol self-assembled monolayers  

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The settlement and adhesion of Navicula perminuta and Ulva linza to methyl-terminated alkanethiol self-assembled monolayers (SAMs) of increasing chain length has been investigated. Organisms were allowed to settle onto the monolayers and were subsequently exposed to hydrodynamic shear stress in order to determine their adhesion strength. Results show that as the SAM structure changes from amorphous to crystalline (C14), there is a marked change in the adhesion of N. perminuta and U. linza. Gi...

Bowen, J.; Pettitt, M. E.; Kendall, K.; Leggett, G. J.; Preece, J. A.; Callow, M. E.; Callow, J. A.

2007-01-01

150

Seaweeds along KwaZulu-Natal coast of South Africa-3: elemental uptake by Ulva lactuca (Sea lettuce).  

Science.gov (United States)

The elemental uptake by Ulva lactuca (Sea lettuce), a marine macro-algae (chlorophyta, green alga) grown richly along KwaZulu-Natal coastline. The total elemental concentrations of seven important elements, namely manganese, iron, arsenic, boron, titanium, zinc and mercury, selected based on their abundance in U. lactuca were investigated for one year cycle (June 2002 to May 2003). The four selected sampling sites, Zinkwasi, Ballito, Treasure Beach and Park Ryrie are spread over 150 km wide along the KwaZulu-Natal coastline from North to South. The Ulva lectuca possess good manganese and arsenic accumulating ability and an excellent bio-indicator for most of the metals studied. A typical U. lectuca sample at Zinkwasi (in winter) recorded Mn (25.3 +/- 1.16 ppm), Fe (21.0 +/- 0.85 ppm), As (6.2 +/- 0.30 ppm), B (935 +/- 14 ppb), Ti (863 +/- 34 ppb), Zn (421 +/- 21 ppb), and Hg (61.3 +/- 1.2 ppb). The general trend found at all sites was high elemental concentrations in winter and a decrease in concentrations from winter to spring and summer. Iron uptake was lowest in summer and autumn at all sites. Ulva lactuca recorded highest mercury levels (>400 ppb) during the spring season at the Treasure Beach site near Durban. PMID:16760099

Misheer, Natasha; Kindness, A; Jonnalagadda, S B

2006-01-01

151

Sulphur fate and anaerobic biodegradation potential during co-digestion of seaweed biomass (Ulva sp.) with pig slurry.  

Science.gov (United States)

Seaweed (Ulva sp.) stranded on beaches were utilized as co-substrate for anaerobic digestion of pig slurry in three-month co-digestion tests in pilot scale anaerobic digesters in the laboratory. The methanogenic potential of Ulva sp. was low compared to that of other potential co-substrates available for use by farmers: 148 N m3CH4/t of volatile solids or 19 N m3CH4/t of crude product. When used as a co-substrate with pig manure (48%/52% w/w), Ulva sp. seaweed did not notably disrupt the process of digestion; however, after pilot stabilisation, biogas produced contained 3.5% H2S, making it unsuitable for energy recovery without treatment. Sequentially addition of the sulphate reduction inhibitor, potassium molybdate, to a final concentration of 3mM, temporarily reduced H2S emissions, but was unable to sustain this reduction over the three-month period. According to these pilot tests, the use of seaweed stranded on beaches as co-substrate in farm-based biogas plants shows some limitations. PMID:21982451

Peu, P; Sassi, J-F; Girault, R; Picard, S; Saint-Cast, Patricia; Béline, F; Dabert, P

2011-12-01

152

Biomasa de Ulva spp. (Chlorophyta) en tres localidades del malecón de La Paz, Baja California Sur, México / Biomass of Ulva spp. (Chlorophyta) in three locations along the bayfront of La Paz, Baja California Sur, México  

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Full Text Available SciELO Chile | Language: Spanish Abstract in spanish Se determinó la distribución, biomasa y potencial cosechable de Ulva spp. en tres localidades del malecón de La Paz, Baja California Sur, durante 2001 y 2002. Algunas especies de este género, antes en el género Enteromorpha, tienen una buena demanda en otros países para consumo humano. Se realizó un [...] muestreo sistemático utilizando como unidad de muestreo cuadros de 25 x 25 cm colocados cada 10 m a lo largo de transectos perpendiculares a la línea de costa trazados cada 40 m. En ambos años la mayor biomasa promedio se encontró en El Palmar. La mayor biomasa de Ulva spp. se registró durante mayo de 2001 y en febrero de 2002. La biomasa promedio fue de 351 g m-2 en 2001, mientras que en 2002 fue de 537 g m-2. Los máximos valores de biomasa cosechable se encontraron en El Kiwi en ambos años. El gran incremento en substrato que se presentó en 2002 podría explicar los altos volúmenes de cosecha estimados de 115 t húmedas. Recomendamos investigar si hay mercado para esta biomasa que permita su explotación sostenida Abstract in english Distribution and standing crop of Ulva spp. were measured in three localities along the bayfront of La Paz, Baja California Sur, during 2001 and 2002. Some species of Ulva, formerly in the genus Enteromorpha, are utilized in some countries for human consumption. We utilized a systematic sampling met [...] hod, using as sampling unit a 25 x 25 cm square placed every 10 m throughout transects perpendicular to the coastline which were drawn every 40 m. In both years the greatest average biomass was found at El Palmar. The largest biomass of Ulva spp. was recorded during May 2001 and February 2002. The average biomass was 351 g m-2 in 2001 and 537 g m-2 in 2002. The maximum values of the standing crop were found at El Kiwi in both years. The great increase in substratum in 2002 may explain the large volumes of the standing crop, estimated as 115 t wet. We recommend investigation to see if there is a market for this biomass leading to its sustainable exploitation

Ruth N, Aguila-Ramírez; Margarita, Casas-Valdez; Claudia J, Hernández-Guerrero; Alejandro, Marín-Álvarez.

2005-07-01

153

Fatty acid profiles indicate the habitat of mud snails Hydrobia ulvae within the same estuary: Mudflats vs. seagrass meadows  

Science.gov (United States)

Mud snails Hydrobia ulvae occupy different habitats in complex estuarine ecosystems. In order to determine if fatty acid profiles displayed by mud snails can be used to identify the habitat that they occupy within the same estuary, fatty acids of H. ulvae from one mudflat and one seagrass meadow in the Ria de Aveiro (Portugal) were analyzed and compared to those displayed by microphytobenthos (MPB), the green leaves (epiphyte-free) of Zostera noltii, as well as those exhibited by the epiphytic community colonizing this seagrass. MPB and epiphytic diatom-dominated samples displayed characteristic fatty acids, such as 16:1 n-7 and 20:5 n-3, while 18:2 n-6 and 18:3 n-3 were the dominant fatty acids in the green leaves of Z. noltii. Significant differences between the fatty acid profiles of H. ulvae specimens sampled in the mudflat and the seagrass meadow could be identified, with those from the mudflat displaying higher levels of fatty acids known to be characteristic of MPB. This result points towards the well known existence of grazing activity on MPB by mud snails. The fatty acid profiles displayed by H. ulvae inhabiting the seagrass meadows show no evidence of direct bioaccumulation of the two most abundant polyunsaturated fatty acids of Z. noltii (18:2 n-6 and 18:3 n-3) in the mud snails, which probably indicates that either these compounds can be metabolized to produce energy, used as precursors for the synthesis of essential fatty acids, or that the snails do not consume seagrass leaves at all. Moreover, the fatty acid profiles of mud snails inhabiting the seagrass meadows revealed the existence of substantial inputs from microalgae, suggesting that the epiphytic community colonizing the leaves of Z. noltii displays an important role on the diet of these organisms. This assumption is supported by the high levels of 20:5 n-3 and 22:6 n-3 recorded in mud snails sampled from seagrass meadows. In conclusion, fatty acid analyses of H. ulvae can be successfully used to identify the habitat occupied by these organisms within the same estuary (e.g. mudflats and seagrass meadows) and reveal the existence of contrasting dietary regimes.

Coelho, Helena; Lopes da Silva, Teresa; Reis, Alberto; Queiroga, Henrique; Serôdio, João; Calado, Ricardo

2011-03-01

154

A LC-MS/MS methodology to determine furaltadone residues in the macroalgae Ulva lactuca.  

Science.gov (United States)

Presently, the rise of new contaminants in the environment has widened the scope of pharmaceutical analyses as to face the demanding new challenges. An increasing tendency for the interconnection and overlap of research fields, such as ecology and biochemistry, is intensifying the demand for new methodologies to be applied to the survey of drugs in unconventional matrices. Integrated in this group are macrophytes, such as the green macroalgae Ulva lactuca, which are under study as to ascertain their ability as indicators of contamination for many substances. Nonetheless, methodologies for extraction and determination of drugs in such matrices are scarce and new studies on the subject are pressing. A new methodology for the determination of the antibiotic furaltadone in U. lactuca by liquid chromatography-tandem mass spectrometric (LC-MS/MS) procedure was developed, optimized and validated following the guidelines of the EC Decision 2002/657. The calibration curves showed linearity above 0.99 (R(2)). The relative standard deviations obtained for repeatability, expressed as CV, were between 15.3 and 20.5 and for reproducibility 25.3 and 28.2 whereas accuracy was in the interval of 88.9-95.5 (%). The limit of decision (CC?) and the detection capability (CC?) were respectively 5.57 ?g kg(-1) and 10.97 ?g kg(-1). The method was successfully applied to experimental samples. PMID:22105023

Leston, Sara; Nunes, Margarida; Freitas, Andreia; Barbosa, Jorge; Ramos, Fernando; Pardal, Miguel Ângelo

2011-12-15

155

Desiccation induces accumulations of antheraxanthin and zeaxanthin in intertidal macro-alga Ulva pertusa (Chlorophyta).  

Science.gov (United States)

For plants and algae, exposure to high light levels is deleterious to their photosynthetic machineries. It also can accelerate water evaporation and thus potentially lead to drought stress. Most photosynthetic organisms protect themselves against high light caused photodamages by xanthophyll cycle-dependent thermal energy dissipation. It is generally accepted that high light activates xanthophyll cycle. However, the relationship between xanthophyll cycle and drought stress remains ambiguous. Herein, Ulva pertusa (Chlorophyta), a representative perennial intertidal macro-algae species with high drought-tolerant capabilities and simple structures, was used to investigate the operation of xanthophyll cycle during desiccation in air. The results indicate that desiccation under dim light induced accumulation of antheraxanthin (Ax) and zeaxanthin (Zx) at the expense of violaxanthin (Vx). This accumulation could be arrested by dithiothreitol completely and by uncoupler (carbonyl cyanide p-trifluoromethoxyphenylhydrazone) partially, implying the participation of Vx de-epoxidase in conversion of Vx to Ax and Zx. Treatment with inhibitors of electron transport along thylakoid membrane, e.g. DCMU, PG and DBMIB, did not significantly arrest desiccation-induced accumulation of Ax and Zx. We propose that for U. pertusa, besides excess light, desiccation itself could also induce accumulation of Ax and Zx. This accumulation could proceed without electron transport along thylakoid membrane, and is possibly resulting from the reduction of thylakoid lumen volume during desiccation. Considering the pleiotropic effects of Ax and Zx, accumulated Ax and Zx may function in protecting thylakoid membrane and enhancing thermal quenching during emersion in air. PMID:24039824

Xie, Xiujun; Gao, Shan; Gu, Wenhui; Pan, Guanghua; Wang, Guangce

2013-01-01

156

Growth inhibition to three red tide microalgae by extracts of Ulva pertusa  

Science.gov (United States)

Growth inhibition effect of different concentration of distilled water extract and four polar organic solvent (methanol, acetone, ether and chloroform) extracts of Ulva pertusa on three typical red tide microalgae ( Heterosigma akashiwo, Alexandrium tamarense and Prorocentrum micans) were investigated. Liquid-liquid fractionation and HPLC analysis for methanol extract of U. pertusa were carried out. Growth of the three microalgae was significantly inhibited by the distilled water extract of U. pertusa at relatively higher concentration. However, the cells of the three microalgae did not die completely even at high concentration. Methanol extract of U. pertusa showed the highest growth inhibition on the three microalgae, and all the cells of the three microalgae were killed at relatively high concentration. The other three organic solvent extracts of U. pertusa had no apparent effect on the three microalgae. The results of bioassays and HPLC analysis suggested that the inhibitory substances in U. pertusa to the microalgal growth had relatively high polarities. H. akashiwo was the most sensitive one while A. tamarense was the most tolerant one to the growth inhibitory substances.

Qiu, Jin; Shuanglin, Dong; Changyun, Wang

2006-06-01

157

Comparative studies of the pyrolytic and kinetic characteristics of maize straw and the seaweed Ulva pertusa.  

Science.gov (United States)

Seaweed has attracted considerable attention as a potential biofuel feedstock. The pyrolytic and kinetic characteristics of maize straw and the seaweed Ulva pertusa were studied and compared using heating rates of 10, 30 and 50°C min(-1) under an inert atmosphere. The activation energy, and pre-exponential factors were calculated by the Flynn-Wall-Ozawa (FWO), Kissinger-Akahira-Sunose (KAS) and Popescu methods. The kinetic mechanism was deduced by the Popescu method. The results indicate that there are three stages to the pyrolysis; dehydration, primary devolatilization and residual decomposition. There were significant differences in average activation energy, thermal stability, final residuals and reaction rates between the two materials. The primary devolatilization stage of U. pertusa can be described by the Avramic-Erofeev equation (n=3), whereas that of maize straw can be described by the Mampel Power Law (n=2). The average activation energy of maize straw and U. pertusa were 153.0 and 148.7 KJ mol(-1), respectively. The pyrolysis process of U.pertusa would be easier than maize straw. And co-firing of the two biomass may be require less external heat input and improve process stability. There were minor kinetic compensation effects between the pre-exponential factors and the activation energy. PMID:20844751

Ye, Naihao; Li, Demao; Chen, Limei; Zhang, Xiaowen; Xu, Dong

2010-01-01

158

Molecular characteristics and immunomodulatory activities of water-soluble sulfated polysaccharides from Ulva pertusa.  

Science.gov (United States)

Sulfated polysaccharides isolated from Ulva pertusa and fractionated using anion-exchange chromatography were investigated to determine their molecular characteristics and bioactivities. The crude and fractionated polysaccharides (F(1), F(2), and F(3)) were mainly composed of carbohydrates (59.9-65.9%), sulfates (11.6-15.3%), and uronic acid (7.30-16.4%) with small amounts of proteins (1.40-4.80%). Rhamnose (62.5-80.7%) was the major monosaccharide unit of these polysaccharides, with different levels of glucose (13.5-27.4%) and xylose (2.74-11.5%). The polysaccharides contained one or two major subfractions with weight-average molecular mass ranging from 51.1×10(3) to 1,690×10(3) g/mol. The relatively low in vitro anticancer activity of the polysaccharides (22.3-42.4%) suggested that they had little cytotoxicity against the cancer cell line used (AGS). On the other hand, the polysaccharides significantly stimulated Raw 264.7 cells, inducing considerable amounts of nitric oxide and various cytokines production, which suggested that they could be strong immunostimulators. PMID:22191629

Tabarsa, Mehdi; Han, Jung H; Kim, Chul Young; You, Sang Guan

2012-02-01

159

Preparation of polysaccharides in different molecular weights from Ulva pertusa Kjellm (Chorophyta)  

Science.gov (United States)

As molecular weight controls the biological activities of polysaccharides, screening the optimal molecular weight of polysaccharides is important in drug research and application. In this study, hydrogen peroxide was employed as oxidant, and temperature, reaction time, and concentration of polysaccharides and hydrogen peroxide were examined for their effects on the preparation of polysaccharides in different molecular weights from Ulva pertusa. Our experiment suggested that the optimal degradation concentrations for polysaccharides and hydrogen peroxide were 2.5% (w/v) and 8.0% (v/v), respectively. The range of degradation measured by relative viscosity was mainly controlled by temperature and time. Results revealed that 35°C was the optimal temperature for obtaining low-degradation samples, and 50°C was the most favorable temperature to accelerate the reaction to yield highly-degraded samples. Four samples in different molecular weights A, B, C and D were finally prepared. The controllability was evaluated by the relative standard deviation (RSD) of relative viscosity, and the peak molecular weights and the polydispersity indexes (Mw/Mn) of molecular weights were measured by high performance gel permeation chromatography (HPGPC).

Yu, Pengzhan; Zhang, Quanbin; Zhang, Hong; Niu, Xizhen; Li, Zhien

2004-12-01

160

Antihyperlipidemic effects of different molecular weight sulfated polysaccharides from Ulva pertusa (Chlorophyta).  

Science.gov (United States)

Ulvan, a sulfated polysaccharide from Ulva pertusa, was degraded to yield two low molecular weight fractions U1 and U2. The molecular weights of ulvan and its fractions were determined and varied from 151.6 to 28.2 kDa. They were fed to rats on a hypercholesterolemic diet for 21 days to evaluate and compare the antihyperlipidemic actions. Ulvan-based diet significantly lowered the levels of serum total cholesterol (-45.2%, P<0.05) and low density lipoprotein cholesterol (LDL-cholesterol, -54.1%, P<0.05). While U1- and U2-based diets significantly elevated the levels of serum high density lipoprotein cholesterol (HDL-cholesterol, +22.0% for U1, not significant; +61.0% for U2; P<0.05) and reduced triglyceride (TG, -82.4% for U1, -77.7% for U2; P<0.05) in rats as compared to control diet. In addition, consumptions of various ulvans significantly increased fecal bile acid excrement. The results indicated that ulvans with different molecular weights exhibited diverse effects on lipid metabolism. The high molecular weight ulvan was effective in serum total and LDL-cholesterol, whereas low molecular weight fractions were in TG and HDL-cholesterol. The fractions were considered to be more beneficial to hyperlipidemia associated with diabetes over ulvan. PMID:14527817

Pengzhan, Yu; Ning, Li; Xiguang, Liu; Gefei, Zhou; Quanbin, Zhang; Pengcheng, Li

2003-12-01

 
 
 
 
161

Purification and properties of cytochrome b-562.5 from Ulva pertusa.  

Science.gov (United States)

Cytochrome b-562.5 (Ulva pertusa) was extracted from a green alga, U. pertusa, by homogenization of the thalli in phosphate buffer solution. Purification was carried out by acrinol treatment, ammonium sulfate fractionation, DEAE-cellulose and DEAE-Sephadex column chromatographies, and Sephadex gel filtration. Cytochrome b-562.5 has absorption maxima at 562.5 (alpha), 530.5 (beta), 429 (gamma), and 326 nm (delta) in the reduced form and at 537, 415 (gamma), and 275 nm in the oxidized form. The alpha-band of the reduced form is asymmetric with a shoulder at 560 nm, at liquid nitrogen temperature this band splits into two distinct peaks at 562 and 556.5 nm. The absorption maxima of the pyridine ferrohemochrome appear at 556 (alpha), 523 (beta), and 418 nm (gamma). The cytochrome does not combine with carbon monoxide or cyanide. The preparation of the cytochrome shows little peroxidase activity. The cytochrome is oxidized by ferricyanide and reduced by cysteine, ascorbate, and hydrosulfite. Autoxidation of the cytochrome was found to be very slow. The midpoint potential (Em) of the cytochrome was determined by equilibration with the ferro- and ferri-EDTA system to be +0.20 V at pH7.0. The molecular weight of the cytochrome was estimated by Sephadex gel filtration to be 23x10(3). PMID:429258

Sugimura, Y; Yakushiji, E

1979-03-01

162

Effect of UV-B irradiation on interspecific competition between Ulva pertusa and Grateloupia filicina  

Science.gov (United States)

We report the effect of UV-B irradiation (9.6 kJ m-2 day-1) on interspecific competition between two species of macroalgae, Ulva pertusa (U) and Grateloupia filicina (G), in co-culture. Growth of U. pertusa and G. filicina was inhibited by UV-B irradiation in mono-culture and specific growth rate ( ?) declined as a result. Interspecific competition between U. pertusa and G. filicina was closely related to the initial weights when co-cultured. When initial ratios of U. pertusa (U) to G. filicina (G) were U:G=1.2:1 and 1:1, U. pertusa was the dominant algae. When the initial U:G ratio was 1:1.2, G. filicina was competitively dominant in the earlier stage, but U. pertusa grew faster, superseding G. filicina in the later stage. At initial ration U:G = 1:1.4, G. filicina was predominant. Under UV-B irradiation, the competitive ability of G. filicina was weakened and the interspecific competitive balance favored U. pertusa, which suggests that G. filicina was more sensitive to UV-B irradiation. We also probed the potential allelopathic effects between the two species, which led to mutual growth inhibition.

Li, Lixia; Zhang, Peiyu; Zhao, Jiqiang; Zhou, Wenli; Tang, Xuexi

2010-03-01

163

Desiccation Induces Accumulations of Antheraxanthin and Zeaxanthin in Intertidal Macro-Alga Ulva pertusa (Chlorophyta)  

Science.gov (United States)

For plants and algae, exposure to high light levels is deleterious to their photosynthetic machineries. It also can accelerate water evaporation and thus potentially lead to drought stress. Most photosynthetic organisms protect themselves against high light caused photodamages by xanthophyll cycle-dependent thermal energy dissipation. It is generally accepted that high light activates xanthophyll cycle. However, the relationship between xanthophyll cycle and drought stress remains ambiguous. Herein, Ulva pertusa (Chlorophyta), a representative perennial intertidal macro-algae species with high drought-tolerant capabilities and simple structures, was used to investigate the operation of xanthophyll cycle during desiccation in air. The results indicate that desiccation under dim light induced accumulation of antheraxanthin (Ax) and zeaxanthin (Zx) at the expense of violaxanthin (Vx). This accumulation could be arrested by dithiothreitol completely and by uncoupler (carbonyl cyanide p-trifluoromethoxyphenylhydrazone) partially, implying the participation of Vx de-epoxidase in conversion of Vx to Ax and Zx. Treatment with inhibitors of electron transport along thylakoid membrane, e.g. DCMU, PG and DBMIB, did not significantly arrest desiccation-induced accumulation of Ax and Zx. We propose that for U. pertusa, besides excess light, desiccation itself could also induce accumulation of Ax and Zx. This accumulation could proceed without electron transport along thylakoid membrane, and is possibly resulting from the reduction of thylakoid lumen volume during desiccation. Considering the pleiotropic effects of Ax and Zx, accumulated Ax and Zx may function in protecting thylakoid membrane and enhancing thermal quenching during emersion in air. PMID:24039824

Xie, Xiujun; Gao, Shan; Gu, Wenhui; Pan, Guanghua; Wang, Guangce

2013-01-01

164

Growth Promotion of a Sea Water Algae with Sterile, Ulva pertusa Kjellman, Due to Supplemental Lighting  

Science.gov (United States)

Ulva pertusa is widely used as human food and also to feed cultured fish. U. pertusa is expected to be used as a bio-filter for purification of breeding - circulation water in the terrestrial aquaculture of fish. Insufficient sunshine and lower temperature causes bad commercial production. In order to realize stable cultivation of U. pertusa throughout the year, experiments on the growth of a sea water algae with sterile, U. pertusa Kjellman, due to supplemental lighting were performed. An illuminance of 1×104 lx in the treatment areas and 2×104 lx in the control, when the illuminance was 8×104 lx in the greenhouse. Artificial lighting was added during the daytime in treatments 1˜3. The mounting height of each luminaire was adjusted to have a PPFD of 140 ?mol m-2s-1. Each lamp had the R/FR PF ratio of 3.8, 1.2, 0.8. Considering of relationship between the growth and cumulative PPFD including daylight and supplemental lighting, the growth of the experimental area were superior to the control area. U. pertusa can be grown with warm sea water using supplemental lighting. But under the growth environment with daylight and supplemental lighting together, it is difficult to recognize whether the R/FR PF ratio influences photomorphogenesis.

Kosaka, Shinichi; Masuda, Atsunori; Ozawa, Tomoko; Ishiwata, Masaki; Yokoji, Shigemi; Saito, Naoki; Murakami, Katsusuke

165

Effect of Sea Lettuce (Ulva lactuca on Soil Water Holding Capacity  

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Full Text Available In this study, the effect of sea lettuce (Ulva lactuca on soil water-holding capacity and the variation by over time were investigated. Sea lettuce (algae was collected from the Black Sea coast, rinsed with rain water and dried to constant weight. Dried algae were added to soil at rate of 0%, 2.5%, 5%, 10% and 20%. Mixtures were filling the pots and incubated for 0, 20, 40, 60 days at 25 ºC. At the end of the incubation, soil organic matter and water holding capacity were determined. Adding of algae to soil was increased the porosity and water holding capacity. The amount of the organic matter was decreased by the incubation progress. At the 20 and 40 days, micro-porosity was increased, air capacity decreased and water-holding capacity has not changed. At the 60th incubation days, reduction of organic matter content was slowed, air capacity was reached to normal values and water holding capacity was decreased. Total porosity was not changed. At the beginning (0th and the 60th incubation days, the distinctive dose was obtained at 5% for porosity and water holding capacity increases. Increased doses of the above have been limited. The optimum dose of 5% as determined by the addition of algae was increased soil water holding capacity by 48% on average.

Ömer Hulusi Dede

2011-06-01

166

A novel ether-linked phytol-containing digalactosylglycerolipid in the marine green alga, Ulva pertusa.  

Science.gov (United States)

Galactosylglycerolipids (GGLs) and chlorophyll are characteristic components of chloroplast in photosynthetic organisms. Although chlorophyll is anchored to the thylakoid membrane by phytol (tetramethylhexadecenol), this isoprenoid alcohol has never been found as a constituent of GGLs. We here described a novel GGL, in which phytol was linked to the glycerol backbone via an ether linkage. This unique GGL was identified as an Alkaline-resistant and Endogalactosylceramidase (EGALC)-sensitive GlycoLipid (AEGL) in the marine green alga, Ulva pertusa. EGALC is an enzyme that is specific to the R-Gal?/?1-6Gal?1-structure of galactolipids. The structure of U. pertusa AEGL was determined following its purification to 1-O-phytyl-3-O-Gal?1-6Gal?1-sn-glycerol by mass spectrometric and nuclear magnetic resonance analyses. AEGLs were ubiquitously distributed in not only green, but also red and brown marine algae; however, they were rarely detected in terrestrial plants, eukaryotic phytoplankton, or cyanobacteria. PMID:25157808

Ishibashi, Yohei; Nagamatsu, Yusuke; Miyamoto, Tomofumi; Matsunaga, Naoyuki; Okino, Nozomu; Yamaguchi, Kuniko; Ito, Makoto

2014-10-01

167

Consumption and feeding preference of Echinogammarus marinus on two different algae: Fucus vesiculosus and Ulva intestinalis  

Science.gov (United States)

Echinogammarus marinus constitutes the most abundant amphipod species in Fucus spp. assemblages from many North Atlantic estuaries. However, there are some doubts about the real use of fucoids by the amphipod. Whilst some studies report the ingestion of Fucus vesiculosus by E. marinus, others suggest that the amphipod preference for fucoids is mostly related to sheltering rather than feeding, due to the high phlorotannin content of brown algae. The purpose of the present work was to disentangle this issue by checking the consumption rate and feeding preference of E. marinus on F. vesiculosus, its preferential habitat, and on Ulva intestinalis, a green algae abundant in the Mondego estuary (Western Coast of Portugal) and usually considered as highly palatable for herbivores. In a 2-stage laboratorial setup, fresh disks of the two types of algae were offered to E. marinus for three days. Consumption rates were estimated from differences between algal and animal initial and final fresh weights using a control correction factor, while preference was tested by differences in algal consumption rates when no choice was offered (stage 1) and when the two algae were offered simultaneously (stage 2). Results showed that E. marinus effectively consumed fresh F. vesiculosus in much higher amounts than U. intestinalis and significantly preferred to consume F. vesiculosus over U. intestinalis. Therefore, feeding habits must be one of the factors related to the close association of the amphipod with F. vesiculosus, although other factors may also be involved (e.g. sheltering).

Martins, Irene; Leite, Nuno; Constantino, Emanuel

2014-01-01

168

Role of Ulva lactuca Extract in Alleviation of Salinity Stress on Wheat Seedlings  

Science.gov (United States)

Seaweeds are potentially excellent sources of highly bioactive materials that could represent useful leads in the alleviation of salinity stress. The effects of presoaking wheat grains in water extract of Ulva lactuca on growth, some enzymatic activities, and protein pattern of salinized plants were investigated in this study. Algal presoaking of grains demonstrated a highly significant enhancement in the percentage of seed germination and growth parameters. The activity of superoxide dismutase (SOD) and catalase (CAT) increased with increasing the algal extract concentration while activity of ascorbate peroxidase (APX) and glutathione reductase (GR) was decreased with increasing concentration of algal extract more than 1% (w/v). The protein pattern of wheat seedling showed 12 newly formed bands as result of algal extract treatments compared with control. The bioactive components in U. lactuca extract such as ascorbic acid, betaine, glutathione, and proline could potentially participate in the alleviation of salinity stress. Therefore, algal presoaking is proved to be an effective technique to improve the growth of wheat seedlings under salt stress conditions.

Ibrahim, Wael M.; Ali, Refaat M.; Hemida, Khaulood A.; Sayed, Makram A.

2014-01-01

169

Distributions of radionuclides among green alga (Ulva pertusa), sea water and marine sediment  

International Nuclear Information System (INIS)

Distributions of radionuclides (60Co, 137Cs, 95Zr-95Nb and 106Ru-106Rh) among green alga (Ulva pertusa), sea water and marine sediment were examined by radioisotope tracer experiment in order to estimate the influence of sediment on the accumulation of radionuclides by the alga. By the application of the compartment model to the experimental results, exponential formulas of distributions were obtained. Through comparison of the transfer coefficients of radionuclides calculated from the exponential formulas, the influence of the sediment on the accumulation of the radionuclides by the green alga was determined to be the largest for 60Co, followed by 95Zr,-95Nb, 106Ru-106Rh and 137Cs in this order. The activity ratios of 95Zr-95Nb and 106Ru-106Rh calculated from the transfer coefficients are larger for the alga than for the sediment, inversely those of 60Co and 137Cs show higher values for the sediment than for the alga. Especially, in the case of 60Co, the activity ratio for the sediment is approximately 20 times greater than that for the alga. Biological half lives in green alga estimated from the transfer coefficients were 10 days for 60Co, 7 days for 137Cs, 26 days for 95Zr-95Nb and 24 days for 95Zr-95Nb and 24 days for 106Ru-106Rh. (auth.)

170

Biosorption of nickel from synthetic and electroplating industrial solutions using a Green marine algae ulva reticulata  

Energy Technology Data Exchange (ETDEWEB)

The present work investigated the biosorption of nickel from synthetic and electroplating industrial effluents using a green marine algae Ulva reticulata. Preliminary batch results imply that pH 4.5 was optimum for nickel uptake and the isotherm experiments conducted at this pH condition indicated that U. reticulata can biosorb 62.3 mg g{sup -1} nickel ions from synthetic solutions, according to the Langmuir model. Desorption was effective and practical using 0.1 M CaCl{sub 2} (pH 2.5, HCl) and the biomass was regenerated and reused for three cycles. Continuous biosorption experiments were performed in an upflow packed column (2 cm I.D and 35 cm height). Among the two electroplating effluents used, effluent-1 is characterized by excess co-ions and high nickel ion content. This influenced the column nickel uptake with U. reticulata exhibiting 52.1 mg g{sup -1} in the case of effluent-1 compared to 56.5 mg g{sup -1} in the case of synthetic solution. On the other hand U. reticulata performed well in effluent-2 with uptakes of 53.3 and 54.3 mg g{sup -1}for effluent-2 and synthetic solution, respectively. Mathematical modeling of column experimental data was performed using nonlinear forms of the Thomas- and modified dose-response models, with the latter able to simulate breakthrough curves with high correlation coefficients. (Abstract Copyright [2008], Wiley Periodicals, Inc.)

Vijayaraghavan, Kuppusamy [Division of Environmental and Chemical Engineering, Chonbuk National University, Chonju (Korea)

2008-03-15

171

Inhibition of growth of Ulva expansa (chlorophyta) by ultraviolet-B radiation  

International Nuclear Information System (INIS)

We examined the effect of ultraviolet-B radiation (UV-B, 290-320 nm) on the growth rate of the intertidal marine alga Ulva expansa (Setch.) S ampersand G. (Chlorophyta). Segments of thallus collected from a natural population were grown in outdoor seawater tanks. Combinations of UV-B-opaque screens, UV-B transparent screens, and UV-B lamps were used to investigate the effects of solar UV-B and solar plus supplemental UV-B on the growth of these segments. Growth was measured by changes in segment surface area, damp weight, and dry weight. Growth rates of segments were inhibited under both solar UV-B and solar plus supplemental UV-B treatments. Growth rates were also inhibited by high levels of photosynthetically active radiation, independent of UV-B fluence. These results indicate that increases in UV-B resulting from further ozone depletion will have a negative impact on the growth of this alga. 32 refs., 5 figs., 2 tabs

172

Seasonality and environmental conditioning in Ulva rigida in Venice lagoon. In situ growth measurement with thalli discs; Stagionalita` e condizionamenti ambientali nello sviluppo di Ulva rigida nella laguna di Venezia. Prove di accrescimento in sity di frammenti di tallo algale  

Energy Technology Data Exchange (ETDEWEB)

The growth trend, the sites and the environmental conditions for the macro algae Ulva rigida have been investigated in Venice lagoon. A method based on in situ growth, using transparent plastic bottles containing thalli discs, and on bi-weekly measurement has been applied for a number of significant lagoon sites. Results pointed out the higher growth period, being from April to June. There was no correlation between the sites where maximum growth was measured and those where algal accumulation used to occur. The effects of some of the environmental factors conditioning algal growth in the different sites have been studied by using cluster analysis.

Rismondo, R.; Zanella, L.; Rismondo, A. [Societa` Biotecnica, Venezia-Mestre (Italy)

1997-11-01

173

An evaluation of the toxicity and bioaccumulation of cisplatin in the marine environment using the macroalga, Ulva lactuca  

Energy Technology Data Exchange (ETDEWEB)

The cytotoxic drug, cisplatin (cis-PtCl{sub 2}(NH{sub 3}){sub 2}), has been added to cultures of the marine macroalga, Ulva lactuca, under various experimental conditions. Both accumulation and internalisation over a 48 h period was greater when cisplatin was added to coastal sea water (salinity = 33) from a distilled water solution than when added to either sea water or estuarine water (salinity = 16.5) from a saline solution. This effect is attributed to the greater abundance of the more reactive monoaqua complex (cis-PtCl(OH{sub 2})(NH{sub 3}){sub 2}{sup +}) in the distilled water solution and kinetic constraints on its conversion back to cis-PtCl{sub 2}(NH{sub 3}){sub 2} in sea water. Despite its mode of action at the cellular level, cisplatin added up to concentrations of 150 nM did not incur a measurable reduction in the efficiency of photochemical energy conversion under any of experimental conditions tested. - Highlights: > This study is the first to examine the biogeochemistry and toxicity of a cytotoxic drug in the marine environment. > Cisplatin is accumulated and internalised by the marine macroalga, Ulva lactuca. > Accumulation is greater when the drug is administered from a distilled water solution than from a saline solution. > Results are consistent with the greater abundance of the more reactive aquated complexes in pure water. > Cisplatin is not phytotoxic to the alga over the concentration range (<150 nM) studied. - The cytotoxic drug, cisplatin, is accumulated and internalised by the marine macroalga, Ulva lactuca, but is not phytotoxic up to concentrations of 150 nM

Easton, Cecilia [School of Geography, Earth and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Turner, Andrew, E-mail: aturner@plymouth.ac.uk [School of Geography, Earth and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Sewell, Graham [School of Health Professions, University of Plymouth, Peninsula Allied Health Centre, Plymouth PL6 8BH (United Kingdom)

2011-12-15

174

An evaluation of the toxicity and bioaccumulation of cisplatin in the marine environment using the macroalga, Ulva lactuca  

International Nuclear Information System (INIS)

The cytotoxic drug, cisplatin (cis-PtCl2(NH3)2), has been added to cultures of the marine macroalga, Ulva lactuca, under various experimental conditions. Both accumulation and internalisation over a 48 h period was greater when cisplatin was added to coastal sea water (salinity = 33) from a distilled water solution than when added to either sea water or estuarine water (salinity = 16.5) from a saline solution. This effect is attributed to the greater abundance of the more reactive monoaqua complex (cis-PtCl(OH2)(NH3)2+) in the distilled water solution and kinetic constraints on its conversion back to cis-PtCl2(NH3)2 in sea water. Despite its mode of action at the cellular level, cisplatin added up to concentrations of 150 nM did not incur a measurable reduction in the efficiency of photochemical energy conversion under any of experimental conditions tested. - Highlights: ? This study is the first to examine the biogeochemistry and toxicity of a cytotoxic drug in the marine environment. ? Cisplatin is accumulated and internalised by the marine macroalga, Ulva lactuca. ? Accumulation is greater when the drug is administered from a distilled water solution than from a saline solution. ? Results are consistent with the greater abundance of the more reactive aquated complexes in pure water. ? Cisplatin is not phytotoxic to the alga over the concentration the alga over the concentration range (<150 nM) studied. - The cytotoxic drug, cisplatin, is accumulated and internalised by the marine macroalga, Ulva lactuca, but is not phytotoxic up to concentrations of 150 nM

175

B?onica oszczepowata Ulva flexuosa subsp. pilifera (Chlorophyta, Ulvophyceae) na nowym s?odkowodnym stanowisku w Poznaniu  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The research site was located on the flow water reservoir in Pozna? (Wielkopolska). The place of U. flexuosa subsp pilifera (Kütz.) Blinding 1963 appearing on the studied area is the 4th inland site of this species in Poland (ATPOL BD09) (Fig. 1). In 2009 the new locality of Ulva flexuosa subsp. pilifera was found in Malta Lake in Pozna? City (Fig. 2). Presence of considerable biomass of U. flexuosa subsp pilifera in this freshwater ecosystem was pointed out to the great fertil...

Rybak, Andrzej; Messyasz, Beata

2011-01-01

176

VARIACIONES EN LA MORFOMETRÍA DE ULVA LACTUCA L (ULVOPHYCEAE EN DOS ZONAS CONTAMINADAS DE LA BAHÍA DE SANTIAGO DE CUBA  

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Full Text Available El presente trabajo expone los resultados obtenidos acerca de la dinámica de crecimiento de la especie Ulva lactuca L en dos zonas contaminadas de la bahía de Santiago de Cuba, durante el período de febrero a mayo de 2007. En cada zona se efectuaron cada 15 días tres transectos para analizar las variaciones morfométricas. El largo de los frondes presentó variaciones entre 0,8 cm y 24,6 cm y el ancho entre 3,0 cm y 42,5 cm. La estación más idónea para el crecimiento de la especie fue Cayo Granma.

Miguel \\u00C1ngel Castell-Puchades

2013-01-01

177

Determination of Heavy Metal Levels in Sediment and Macroalgae (Ulva Sp. and Enteromorpha Sp.) on the Mersin Coast  

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Mersin ili s?n?rlar? içerisinde halk taraf?ndan yo?un olarak kullan?lan dört plajda makroskobik ye?il algler(Ulva sp. ve Enteromorpha sp.) ve sedimentte a??r metallerin birikiminin belirlenmesi amac?yla, Alüminyum(Al), Krom (Cr), Mangan (Mn), Demir (Fe), Nikel (Ni), Bak?r (Cu), Çinko (Zn), Kur?un (Pb), veKadmiyum (Cd) düzeyleri ayl?k olarak tespit edilmi?tir. Yap?lan analizler sonucunda Cu ve Zn istasyon 1,Cd istasyon 2 ve Al, Mn, Fe ve Ni ise istasyon 4’de alglerde en y...

Alp, Mehmet Tahir; Ozgur Ozbay; Sungur, Mehmet Ali

2012-01-01

178

Ocorrência de Ulva spp., Polysiphonia sp., e Microcystis aeruginosa nas praias do Saco do Laranjal, Pelotas, RS  

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Full Text Available O trabalho relata a ocorrência de algas indicadoras de processos de eutrofização no Saco do Laranjal (Lagoa dos Patos no período compreendido entre 2005 e 2012. A ocorrência de grandes quantidades da alga macroscópica verde Ulva L. (Chlorophyta, formando marés verde, foram registradas nos verão de 2008, 2009 e 2012. A cianofícea potencialmente tóxica, Microcystis aeruginosa (Kütz. Kütz. foi observada em quase todos os anos, nos meses de verão e, em floração massiva, no verão de 2010. São fornecidas ilustrações dos eventos de crescimento massivos observados. Os resultados sugerem que o Saco do Laranjal provavelmente apresenta-se em processo de eutrofização.

Vanessa Correa da Rosa

2013-10-01

179

Biosorption of Copper, Nickel and Manganese Using Non-Living Biomass of Marine Alga, Ulva lactuca  

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Full Text Available The adsorption of Cu2+, Ni2+ and Mn2+ onto the marine algal biomass of Ulva lactuca was investigated in single and multimetal solutions. This study was intended to determine the role of different pH values (2-8 on the biosorption of metals at different concentrations (10, 20 and 30 mg L-1. The biosorption capacity of Cu2+, Ni2+ and Mn2+ for 10 mg L-1 was the same as 20 and 30 mg L-1, increase with increasing pH up to pH 5.0 and then decreased, in single and multimetal solutions. The optimum pH value was observed in the pH range 4-5 for Cu2+ and pH 5-6 for Ni2+ and Mn2+. The maximum biosorption capacities of tested alga for Cu2+, Ni2+ and Mn2+ were 92, 80 and 75%, respectively in single metal solution at 10 mg L-1 and pH 5.0. At a further increase of pH (8.0 the biosorption process for Cu2+, Ni2+ and Mn2+ (75, 69 and 63%, respectively at 10 mg L-1 was decreased. The minimum biosorptions were 60, 49 and 44% for Cu2+, Ni2+ and Mn2+, respectively in single metal solution at 10 mg L-1 and pH 2.0. In the multimetal solution, algal biomass exhibited the maximum and the minimum biosorption capacity at different pH values the same as in single metal solution. The inhibitory role of other ions on sorption process can be well observed in multimetal mixture, where biosorption capacity of Cu2+, Ni2+ and Mn2+ were significantly decreased in the multimetal solutions. The maximum biosorption was recorded for Cu2+ (83% in solution of Cu2+ + Mn2+, Mn2+ (67% in solution of Ni2+ + Mn2+ and for Ni2+ (74% in solution of Ni2+ + Mn2+ at the concentration 10 mg L-1 and pH 5.0. The observed reduction in the biosorption of Cu2+, Ni2+ and Mn2+ (65, 57 and 52%, respectively at 10 mg L-1 and pH 5.0 was more pronounced in the multimetal solution of Cu2+ + Ni2+ + Mn2+ as compared with single metal solution. The results demonstrated that the affinity of the tested alga for sorption of the investigated metal ions in single and multimetal solutions runs in the order Cu2+ > Ni2+ > Mn2+. Biosorption equilibrium was established by the Langmuir and Freundlich isotherm models. According to the analyses conducted, the biosorption of Cu2+, Ni2+ and Mn2+ to Ulva lactuca was more consistent with Freundlich isotherm.

Hanan Hafez Omar

2008-01-01

180

On the recurrent Ulva prolifera blooms in the Yellow Sea and East China Sea  

Science.gov (United States)

A massive bloom of the green macroalgae Ulva prolifera (previously known as Enteromorpha prolifera) occurred in June 2008 in the Yellow Sea (YS), resulting in perhaps the largest "green tide" event in history. Using a novel index (Floating Algae Index) and multiresolution remote sensing data from MODIS and Landsat, we show that U. prolifera patches appeared nearly every year between April and July 2000-2009 in the YS and/or East China Sea (ECS), which all originated from the nearshore Subei Bank. A finite volume numerical circulation model, driven by realistic forcing and boundary conditions, confirmed this finding. Analysis of meteorological/environmental data and information related to local aquaculture activities strongly supports the hypothesis that the recurrent U. prolifera in the YS and ECS resulted from aquaculture of the seaweed Porphyra yezoensis (or nori) conducted along the 200 km shoreline of the Subei Bank north of the Changjiang (Yangtze) River mouth. Given the continuous growth in aquaculture efforts in the region, similar macroalgae bloom events, such as the summer 2008 event, are likely to occur in the future, particularly between May and July. This was confirmed by the 2009 bloom event in the same regions and the same period. The profit of the local P. yezoensis aquaculture industry (˜16,000 Ha in 2007) is estimated as U.S. 53 million, yet the cost to manage the impact of the summer 2008 U. prolifera bloom exceeded U.S. 100 million. Therefore, better strategies are required to balance the economic benefit of seaweed aquaculture and the costs of environmental impacts.

Hu, Chuanmin; Li, Daqiu; Chen, Changsheng; Ge, Jianzhong; Muller-Karger, Frank E.; Liu, Junpeng; Yu, Feng; He, Ming-Xia

2010-05-01

 
 
 
 
181

Prevalence and mechanism of polyunsaturated aldehydes production in the green tide forming macroalgal genus Ulva (Ulvales, Chlorophyta).  

Science.gov (United States)

Lipoxygenase/hydroperoxide lyase mediated transformations convert polyunsaturated fatty acids into various oxylipins. First, lipoxygenases catalyze fatty acid oxidation to fatty acid hydroperoxides. Subsequently, breakdown reactions result in a wide array of metabolites with multiple physiological and ecological functions. These fatty acid transformations are highly diverse in marine algae and play a crucial rule in e.g., signaling, chemical defense, and stress response often mediated through polyunsaturated aldehydes (PUAs). In this study, green tide-forming macroalgae of the genius Ulva (Chlorophyta) were collected at various sampling sites in the lagoon of the Ria Formosa (Portugal) and were surveyed for PUAs. We demonstrated that sea-lettuce like but not tube-like morphotypes produce elevated amounts of volatile C10-polyunsaturated aldehydes (2,4,7-decatrienal and 2,4-decadienal) upon tissue damage. Moreover, morphogenetic and phylogenetic analyses of the collected Ulva species revealed chemotaxonomic significance of the perspective biosynthetic pathways. The aldehydes are derived from omega-3 and omega-6 polyunsaturated fatty acids (PUFA) with 20 or 18 carbon atoms including eicosapentaenoic acid (C20:5 n-3), arachidonic acid (C20:4 n-6), stearidonic acid (C18:4 n-3), and ?-linolenic acid (C18:3 n-6). We present first evidences that lipoxygenase-mediated (11-LOX and 9-LOX) eicosanoid and octadecanoid pathways catalyze the transformation of C20- and C18-polyunsaturated fatty acids into PUAs and concomitantly into short chain hydroxylated fatty acids. PMID:24915501

Alsufyani, Taghreed; Engelen, Aschwin H; Diekmann, Onno E; Kuegler, Stefan; Wichard, Thomas

2014-10-01

182

Levels of essential and toxic elements in Porphyra columbina and Ulva sp. from San Jorge Gulf, Patagonia Argentina  

Energy Technology Data Exchange (ETDEWEB)

Baseline concentration levels of As, B, Ca, Cd, Cr, Cu, Co, Fe, Mg, Mn, Mo, Ni, P, Pb, Se, V, and Zn were determined for Porphyra columbina and Ulva sp. collected from three locations along San Jorge Gulf, in Patagonia Argentina. Elements were quantified by inductively coupled plasma-optical emission spectrometry, with the exception of lead and cadmium in some samples which were determined by electrothermal atomic absorption spectrometry. Three stations with different exposure degree to human activities, Bahia Solano, the mouth of Arroyo La Mata stream and Punta Maqueda, were selected as sampling points. The results showed a wide range of metal retention capacity between the two studied species. Regarding the levels of pollutants found in the researched sites, Punta Maqueda seemed to be less influenced by anthropogenic activities than the other two sites except for Cd. Taking into account their toxicities seasonal variations in Pb and Cd levels were studied in both algae in Punta Maqueda. Maximum concentrations of Cd (9.8 {mu}g g{sup -1} dry wt.) were observed in P. columbina during winter, and maximum levels of Pb (0.82 {mu}g g{sup -1} dry wt.) were detected in Ulva sp. during summer. Legislative and health safety aspects were evaluated for Cd and Pb.

Perez, Adriana Angela [Catedras de Toxicologia y Quimica Legal, Biologia y Bromatologia y Nutricion, Universidad Nacional de la Patagonia San Juan Bosco, Chubut (Argentina); Farias, Silvia Sara [Comision Nacional de Energia Atomica, Gerencia de Tecnologia y Medio Ambiente, Buenos Aires (Argentina); Strobl, Analia Mabel [Catedras de Toxicologia y Quimica Legal, Biologia y Bromatologia y Nutricion, Universidad Nacional de la Patagonia San Juan Bosco, Chubut (Argentina); Perez, Laura Beatriz [Catedras de Toxicologia y Quimica Legal, Biologia y Bromatologia y Nutricion, Universidad Nacional de la Patagonia San Juan Bosco, Chubut (Argentina); Lopez, Clara Magdalena [Catedra de Toxicologia y Quimica Legal, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires (Argentina); Pineiro, Adriana [Catedra de Toxicologia y Quimica Legal, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires (Argentina); Roses, Otmaro [Catedra de Toxicologia y Quimica Legal, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires (Argentina); Fajardo, Maria Angelica [Catedras de Toxicologia y Quimica Legal, Biologia y Bromatologia y Nutricion, Universidad Nacional de la Patagonia San Juan Bosco, Chubut (Argentina)]. E-mail: copipat@sinectis.com.ar

2007-04-15

183

The appearance of Ulva laetevirens (Ulvophyceae, Chlorophyta) in the northeast coast of the United States of America  

Science.gov (United States)

Introduced species may outcompete or hybridize with native species, resulting in the loss of native biodiversity or even alteration of ecosystem processes. In this study, we reported an alien distromatic Ulva species, which was found in an embayment (Holly Pond) connected with Long Island Sound, USA. The morphological and anatomical observations in combination with molecular data were used for its identification to species. Anatomy of collected specimens showed that the cell shape in rhizoidal and basal regions was round and the marginal teeth along the basal and median region were not found. These characteristics were primarily identical to the diagnostic characteristics of Ulva laetevirens Areschoug (Chlorophyta). The plastid-encoding tufA and nucleusencoding ITS1 were used for its molecular identification. Phylogenetic analysis for the tufA gene placed the specimens from Holly Pond in a well-supported clade along with published sequences of U. laetevirens identified early without any sequence divergence. In ITS tree, the sample also formed well-supported clades with the sequences of U. laetevirens with an estimated sequence divergence among the taxa in these clades as low as 1%. These findings confirmed the morpho-anatomical conclusion. Native to Australia, this species was reported in several countries along the Mediterranean coast after the late of 1990s. This is the first time that U. laetevirens is found in the northeast coast of United States and the second record for Atlantic North America.

Mao, Yunxiang; Kim, Jang Kyun; Wilson, Roderick; Yarish, Charles

2014-10-01

184

Levels of essential and toxic elements in Porphyra columbina and Ulva sp. from San Jorge Gulf, Patagonia Argentina  

International Nuclear Information System (INIS)

Baseline concentration levels of As, B, Ca, Cd, Cr, Cu, Co, Fe, Mg, Mn, Mo, Ni, P, Pb, Se, V, and Zn were determined for Porphyra columbina and Ulva sp. collected from three locations along San Jorge Gulf, in Patagonia Argentina. Elements were quantified by inductively coupled plasma-optical emission spectrometry, with the exception of lead and cadmium in some samples which were determined by electrothermal atomic absorption spectrometry. Three stations with different exposure degree to human activities, Bahia Solano, the mouth of Arroyo La Mata stream and Punta Maqueda, were selected as sampling points. The results showed a wide range of metal retention capacity between the two studied species. Regarding the levels of pollutants found in the researched sites, Punta Maqueda seemed to be less influenced by anthropogenic activities than the other two sites except for Cd. Taking into account their toxicities seasonal variations in Pb and Cd levels were studied in both algae in Punta Maqueda. Maximum concentrations of Cd (9.8 ?g g-1 dry wt.) were observed in P. columbina during winter, and maximum levels of Pb (0.82 ?g g-1 dry wt.) were detected in Ulva sp. during summer. Legislative and health safety aspects were evaluated for Cd and Pb

185

Determination of Heavy Metal Levels in Sediment and Macroalgae (Ulva Sp. and Enteromorpha Sp. on the Mersin Coast  

Directory of Open Access Journals (Sweden)

Full Text Available Mersin ili s?n?rlar? içerisinde halk taraf?ndan yo?un olarak kullan?lan dört plajda makroskobik ye?il algler(Ulva sp. ve Enteromorpha sp. ve sedimentte a??r metallerin birikiminin belirlenmesi amac?yla, Alüminyum(Al, Krom (Cr, Mangan (Mn, Demir (Fe, Nikel (Ni, Bak?r (Cu, Çinko (Zn, Kur?un (Pb, veKadmiyum (Cd düzeyleri ayl?k olarak tespit edilmi?tir. Yap?lan analizler sonucunda Cu ve Zn istasyon 1,Cd istasyon 2 ve Al, Mn, Fe ve Ni ise istasyon 4’de alglerde en yüksek birikimi gösteren a??r metallerolmu?lard?r. A??r metallerin birikim düzeylerinin alglerde ve sedimentte s?ralanmas?, Ulva sp.’de Fe>Al>Mn>Ni>Zn>Cr>Cu>Pb>Cd; Enteromorpha sp.’de Fe>Al>Ni>Mn>Zn>Cr>Cu>Pb>Cd;sediment örneklerinde ise Fe>Al>Mn>Ni>Cr>Zn>Pb>Cd>Cu ?eklinde tespit edilmi?tir.

Mehmet Tahir ALP

2012-03-01

186

Macroalgae mitigation potential for fish aquaculture effluents: an approach coupling nitrogen uptake and metabolic pathways using Ulva rigida and Enteromorpha clathrata.  

Science.gov (United States)

Aquaculture effluents are rich in nitrogen compounds that may enhance local primary productivity, leading to the development of algae blooms. The goal of this study was to assess the potential use of naturally occurring green macroalgae (Ulva and Enteromorpha) as bioremediators for nitrogen-rich effluents from a fish aquaculture plant, by evaluating their respective uptake dynamics under controlled conditions. Ulva and Enteromorpha were incubated separately in aquaculture effluent from a local pilot station. Algae tissue and water samples were collected periodically along 4 h. For each sample, nitrate, nitrite, and ammonia concentrations were quantified in the effluent, while internal algae reserve pools and nitrate reductase activity (NRA) were determined within the algae tissues. Both macroalgae absorbed all dissolved inorganic nitrogen compounds in less than 1 h, favoring ammonia over nitrate. Ulva stored nitrate temporarily as an internal reserve and only used it after ammonia availability decreased, whereas Enteromorpha stored and metabolized ammonia and nitrate simultaneously. These distinct dynamics of ammonia and nitrate uptake supported an increase in NRA during the experiment. This study supports the hypothesis that Ulva or Enteromorpha can be used as bioremediators in aquaculture effluents to mitigate excess of dissolved inorganic nitrogen. PMID:24338110

Aníbal, Jaime; Madeira, Hélder T; Carvalho, Liliana F; Esteves, Eduardo; Veiga-Pires, Cristina; Rocha, Carlos

2014-12-01

187

[Response of interspecific competition between Ulva pertusa and Grateloupia filicina to UV-B irradiation enhancement].  

Science.gov (United States)

The interspecific competition between Ulva pertusa and Grateloupia filicina and it's response to the UV-B irradiation enhancement were analyzed using mono-culture and co-culture methods. The study adopted reasonable experimental design and took biomass as the main examined index. Results showed that the relation of interspecific competition included both allelopathy effect and nutrient competition. Specific growth rates of U. pertusa under treatment with abundant nutrition and limited nutrition was 2.54 and 2.47 times of those of G. filicina. Thus, compared to U. pertusa, G. filicina was in inferior position. UV-B irradiation could inhibit the growth of U. pertusa and G. filicina under the condition of mono-culture. The higher the dosage and the longer exposure of UV-B irradiation were, the more significant the inhibitive effect was. When they were cultured together, low dosage [1.6 kJ x (m2 x d)(-1)] and medium dosage [4.8 kJ x(m2 x d)(-1)] of UV-B irradiation reduced the competitive ability of U. pertusa, and weights of U. pertusa and G. filicina declined 6.81% and 3.88% in low dosage, and 10.47% and 6.98% in medium dosage, respectively. So the relation of interspecific competition tended to be at a balanced level even though U. pertusa was still the dominant algae. However, on the 12th day, weight of U. pertusa decreased by 13.09%, but the value of G. filicina was 14.72%, which was higher than that of U. pertusa. Therefore, high dosage [9.6 kJ x (m2 x d)(-1)] of UV-B irradiation had more serious inhibitive effect on G. filicina, and competitive dominant position of U. pertusa tended to be more obvious. Thus, UV-B changed the relation of competitive balance of U. pertusa and G. filicina, which changed along with the dosage of UV-B. Moreover, UV-B irradiation might influence the metabolism of the allelochemicals produced by U. pertusa and G. filicina in a long time. PMID:19143368

Li, Li-Xia; Dong, Kai-Sheng; Tang, Xue-Xi

2008-10-01

188

Effects of ulvoid (Ulva spp.) accumulation on the structure and function of eelgrass (Zostera marina L.) bed.  

Science.gov (United States)

The objective of this study is to clarify the effect of ulvoid (Ulva spp.) accumulation on the structure and function of an eelgrass bed by the coast of Iwakuni, Seto Inland Sea, Japan. We monitored eelgrass shoot density and volume of ulvoid accumulation in the study site and evaluated effects of the accumulated ulvoid canopy on the percent survival, seedling density, growth rates, photosynthetic photon flux density (PPFD) and carbon contents of eelgrass. Eelgrass shoot density decreased by the accumulation of ulvoid. Also, seedling density decreased by the increase in the ulvoid volumes. Shoot density, seedling density and leaf elongation were negatively correlated with ulvoid volume. Carbon contents in eelgrass decreased by the accumulation of ulvoid (canopy height: 25cm). These results suggest that accumulation of ulvoid bloom has significant negative impacts on the structure and function of eelgrass bed, i.e. decreases in vegetative shoot density, seedling density, shoot height and growth rate. PMID:17692339

Sugimoto, Kenji; Hiraoka, Kiyonori; Ohta, Seiji; Niimura, Yoko; Terawaki, Toshinobu; Okada, Mitsumasa

2007-10-01

189

Comparative studies on phosphorus uptake and growth kinetics of the microalga Tetraselmis subcordiformis and the macroalga Ulva pertusa  

Science.gov (United States)

Short-term uptake experiments and long-term semicontinuous culture experiments were performed under the condition of phosphorus (P) limitation to estimate and compare the P uptake and growth kinetics of the microalga Tetraselmis subcordiformis and the macroalga Ulva pertusa. Two new parameters, the maximum specific uptake rate ( V {m/sp}) and the maximal growth efficiency (?), are introduced to achieve uniformity for the comparison of nutrient uptake and growth efficiency between microalgae and macroalgae. T. subcordiformis possesses 3 times lower half saturation uptake constant, 4 times higher maximal growth rate and 20 times higher maximum specific uptake rate than U. pertusa, while U. pertusa possesses 4 times higher maximal growth efficiency than T. subcordiformis.

Nan, Chunrong; Dong, Shuanglin

2004-04-01

190

In vivo anti-radiation activities of the Ulva pertusa polysaccharides and polysaccharide-iron(III) complex.  

Science.gov (United States)

Polysaccharides with different molecular weights were extracted from Ulva pertusa and fractionated by ultrafiltration. Iron(III) complex of the low molecular-weight U. pertusa polysaccharides were synthesized. Atomic absorption spectrum showed that the iron content of iron(III)-polysaccharide complex was 27.4%. The comparison between U. pertusa polysaccharides and their iron(III) complex showed that iron chelating altered the structural characteristics of the polysaccharides. The bioactivity analysis showed that polysaccharide with low molecular weight was more effective than polysaccharide with high molecular weight in protecting mice from radiation induced damages on bone marrow cells and immune system. Results also proved that the anti-radiation and anti-oxidative activity of iron(III) complex of low molecular-weight polysaccharides were not less than that of low molecular-weight polysaccharides. PMID:23751317

Shi, Jinming; Cheng, Cuilin; Zhao, Haitian; Jing, Jing; Gong, Ning; Lu, Weihong

2013-09-01

191

Biosorption of bovine serum albumin by Ulva lactuca biomass from industrial wastewater: Equilibrium, kinetic and thermodynamic study  

International Nuclear Information System (INIS)

Batch biosorption experiments have been carried out for the removal of bovine serum albumin (BSA) from simulated industrial wastewater onto Ulva lactuca seaweed. Various vital parameters influencing the biosorption process such as initial concentration of BSA, pH of the solution, adsorbent dosage and temperature have been determined. The biosorption kinetics follows a pseudo-second order kinetic model. Equilibrium isotherm studies demonstrate that the biosorption followed the Freundlich isotherm model, which implies a heterogeneous sorption phenomenon. Various thermodynamic parameters such as changes in enthalpy, free energy and entropy have been calculated. The positive value of ?Ho and the negative value of ?Go show that the sorption process is endothermic and spontaneous. The positive value of change in entropy ?So shows increased randomness at the solid-liquid interface during the biosorption of BSA onto U. lactuca seaweed.

192

Effects of ulvoid (Ulva spp.) accumulation on the structure and function of eelgrass (Zostera marina L.) bed  

International Nuclear Information System (INIS)

The objective of this study is to clarify the effect of ulvoid (Ulva spp.) accumulation on the structure and function of an eelgrass bed by the coast of Iwakuni, Seto Inland Sea, Japan. We monitored eelgrass shoot density and volume of ulvoid accumulation in the study site and evaluated effects of the accumulated ulvoid canopy on the percent survival, seedling density, growth rates, photosynthetic photon flux density (PPFD) and carbon contents of eelgrass. Eelgrass shoot density decreased by the accumulation of ulvoid. Also, seedling density decreased by the increase in the ulvoid volumes. Shoot density, seedling density and leaf elongation were negatively correlated with ulvoid volume. Carbon contents in eelgrass decreased by the accumulation of ulvoid (canopy height: 25 cm). These results suggest that accumulation of ulvoid bloom has significant negative impacts on the structure and function of eelgrass bed, i.e. decreases in vegetative shoot density, seedling density, shoot height and growth rate

193

Biosorption of bovine serum albumin by Ulva lactuca biomass from industrial wastewater: Equilibrium, kinetic and thermodynamic study  

Energy Technology Data Exchange (ETDEWEB)

Batch biosorption experiments have been carried out for the removal of bovine serum albumin (BSA) from simulated industrial wastewater onto Ulva lactuca seaweed. Various vital parameters influencing the biosorption process such as initial concentration of BSA, pH of the solution, adsorbent dosage and temperature have been determined. The biosorption kinetics follows a pseudo-second order kinetic model. Equilibrium isotherm studies demonstrate that the biosorption followed the Freundlich isotherm model, which implies a heterogeneous sorption phenomenon. Various thermodynamic parameters such as changes in enthalpy, free energy and entropy have been calculated. The positive value of {Delta}H{sup o} and the negative value of {Delta}G{sup o} show that the sorption process is endothermic and spontaneous. The positive value of change in entropy {Delta}S{sup o} shows increased randomness at the solid-liquid interface during the biosorption of BSA onto U. lactuca seaweed.

Rathinam, Aravindhan [Chemical Laboratory, Central Leather Research Institute, Adyar, Chennai 600020 (India); Zou, Linda, E-mail: linda.zou@unisa.edu.au [SA Water Centre for Water Management and Reuse, University of South Australia, Mawson Lakes Campus, Adelaide, SA 5095 (Australia)

2010-12-15

194

Effects of ulvoid (Ulva spp.) accumulation on the structure and function of eelgrass (Zostera marina L.) bed  

Energy Technology Data Exchange (ETDEWEB)

The objective of this study is to clarify the effect of ulvoid (Ulva spp.) accumulation on the structure and function of an eelgrass bed by the coast of Iwakuni, Seto Inland Sea, Japan. We monitored eelgrass shoot density and volume of ulvoid accumulation in the study site and evaluated effects of the accumulated ulvoid canopy on the percent survival, seedling density, growth rates, photosynthetic photon flux density (PPFD) and carbon contents of eelgrass. Eelgrass shoot density decreased by the accumulation of ulvoid. Also, seedling density decreased by the increase in the ulvoid volumes. Shoot density, seedling density and leaf elongation were negatively correlated with ulvoid volume. Carbon contents in eelgrass decreased by the accumulation of ulvoid (canopy height: 25 cm). These results suggest that accumulation of ulvoid bloom has significant negative impacts on the structure and function of eelgrass bed, i.e. decreases in vegetative shoot density, seedling density, shoot height and growth rate.

Sugimoto, Kenji [Hiroshima Environment and Health Association, 9-11, Hirosekitamachi Naka-ku, Hiroshima 730-8631 (Japan)], E-mail: kenji.sugimoto@kanhokyo.or.jp; Hiraoka, Kiyonori [Hiroshima Environment and Health Association, 9-11, Hirosekitamachi Naka-ku, Hiroshima 730-8631 (Japan); Ohta, Seiji [Hiroshima Defense Facilities Administration Bureau, 6-30, Kamihattyobori, Naka-ku, Hiroshima 730-0012 (Japan); Niimura, Yoko [National Research Institute of Fisheries and Environment of Inland Sea, 2-17-5 Maruishi Ohno, Saeki, Hiroshima 739-0452 (Japan); Terawaki, Toshinobu [Fisheries Research Agency, Queen' s Tower-B 15F, Minato-Mirai, West, Yokohama 220-6115 (Japan); Okada, Mitsumasa [Hiroshima University, 1-4-1, Kagamiyama, Higashihiroshima 739-8527 (Japan)

2007-10-15

195

Screening of priority pesticides in Ulva sp. seaweeds by selective pressurized solvent extraction before gas chromatography with electron capture detector analysis.  

Science.gov (United States)

This work reports a fast and reliable analytical method for the screening of priority pesticides (PPs) in Ulva sp. seaweeds by gas chromatography with electron capture detection. Extraction and sample clean-up were performed in one single step by selective pressurized liquid extraction (SPLE). Several parameters affecting SPLE performance were optimized. Method performance was compared with standard Soxhlet extraction. Significant decrease of the time of analysis with better recoveries for a greater number of PPs was achieved by SPLE. Average recoveries ranged from 71 to 103% with RSD Ulva sp. seaweeds tend to accumulate PPs and have the potential to be used as early alert signals of aquatic pollution especially after rains and storm events. PMID:24854703

Pinto, M I; Micaelo, C; Vale, C; Sontag, G; Noronha, J P

2014-11-01

196

Effects of desiccation and salinity on the outbreak of a green tide of Ulva pertusa in a created salt marsh along the coast of Osaka Bay, Japan  

Science.gov (United States)

Field surveys and laboratory experiments were conducted to examine the effect of desiccation and salinity on the outbreak of a green tide of Ulva pertusa at Osaka Nanko bird sanctuary. Reduction of biomass of Ulva spp. was observed at stations where the exposure rate to air was from 30 to 40%. In addition, the exposure rate of 30-40% to air showed no negative impacts on the biomass of benthic microalgae, infauna and non-motile epibenthos. Laboratory experiments revealed that photosynthetic activity of U. pertusa decreased when exposed to air for 4-7 h at 25-35 °C. Salinity decreases from 30 to 25 or 20 accompanied with exposure to air drastically reduced the rate of photosynthesis of this species. These results suggest the possibility of controlling a green tide of U. pertusa without serious physico-ecological damage to benthic microalgae, infauna and non-motile epibenthos by a combination of exposure to air with low salinity.

Yamochi, Susumu

2013-01-01

197

Estrutura populacional de Hyale media (Dana (Amphipoda, Gammaridea, Hyalidae, habitante dos fitais de Caiobá, Matinhos, Paraná, Brasil Population structure of the seaweed dweller Hyale media (Dana (Amphipoda, Gammaridea, Hyalidae from Caiobá, Matinhos, Paraná, Brazil  

Directory of Open Access Journals (Sweden)

Full Text Available A study of correlation between the total body length and the somites length was carried out in a population of Hyale media (Dana, 1857, in order to know which somite or group of somites has the highest correlation index with the total body length. As the sum of the length of the first to fourth pereonites showed the highest linear correlation index (Y=0.0764+0.2736X; r=0.9723, this meristic parameter was chosen to describe the population structure of the species. The following aspects were treated: distribution of the body size classes in the various phytals, population composition, seasonal fluctuation of population density. relative frequency of the ovigerous females and correlation between the body length and the number of eggs inside the marsupium of the ovigerous females. The amphipods were obtained from the seasonal collections of six phytals from a rocky seashore of Caiobá, Paraná State: Pterosiphonia pennata (Roth Falkenberg. Gymrogongrus griffithsiae (Turner Martius, Pterocladia capillacea (Gmelin Bornet & Thured, Sargassum cymosum Garth, Gelidium sp and Ulva fasciata Delile; they did not occurred in Padina gymnospora (Kútsing Vickers and Porphyra atropurpurea (Olivi De Toni. The air temperature oscillated from 16ºC (winter and autumn to 23ºC (summer, the surface water temperature from 17ºC (winter to 25ºC (summer and the surface water salinity, from 29.3‰ (autumn to 32.8‰ (winter. The density oi Hyale media varied from 0.20 ind.g-1 (in Ulva to 26.37 ind.g-1 (in Pterosiphonia of alga-substratum weigth, and the population was distributed mainly in branched algae. It was determined three size classes in the population, within a range from 0.01 to 2.99mm of pereonits 1-4 length. Small amphipods prefer finely branched algae like Gymnogongrusand Pterosiphonia, whereas broad-thallii or less branched algae such as Sargassum, Pterocladia, Gelidium and Ulva harbour proporcionally high number of large individuais. The life cycle of Hyale media takes place wholly in the phytals and the species reproduces continually all year round: males, ovigerous females and juveniles are present every season. The highest femalc reproductive activity occurs in winter and the juveniles are more numerous in summer. The number of eggs inside the marsupium and the pereonites 1-4 length has a linear correlation (Y=-9,9682+12,0729X; r=0.8024.

Janete Dubiaski-Silva

1998-01-01

198

Estrutura populacional de Hyale media (Dana) (Amphipoda, Gammaridea, Hyalidae), habitante dos fitais de Caiobá, Matinhos, Paraná, Brasil / Population structure of the seaweed dweller Hyale media (Dana) (Amphipoda, Gammaridea, Hyalidae) from Caiobá, Matinhos, Paraná, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese [...] Abstract in english A study of correlation between the total body length and the somites length was carried out in a population of Hyale media (Dana, 1857), in order to know which somite or group of somites has the highest correlation index with the total body length. As the sum of the length of the first to fourth per [...] eonites showed the highest linear correlation index (Y=0.0764+0.2736X; r=0.9723), this meristic parameter was chosen to describe the population structure of the species. The following aspects were treated: distribution of the body size classes in the various phytals, population composition, seasonal fluctuation of population density. relative frequency of the ovigerous females and correlation between the body length and the number of eggs inside the marsupium of the ovigerous females. The amphipods were obtained from the seasonal collections of six phytals from a rocky seashore of Caiobá, Paraná State: Pterosiphonia pennata (Roth) Falkenberg. Gymrogongrus griffithsiae (Turner) Martius, Pterocladia capillacea (Gmelin) Bornet & Thured, Sargassum cymosum Garth, Gelidium sp and Ulva fasciata Delile; they did not occurred in Padina gymnospora (Kútsing) Vickers and Porphyra atropurpurea (Olivi) De Toni. The air temperature oscillated from 16ºC (winter and autumn) to 23ºC (summer), the surface water temperature from 17ºC (winter) to 25ºC (summer) and the surface water salinity, from 29.3‰ (autumn) to 32.8‰ (winter). The density oi Hyale media varied from 0.20 ind.g-1 (in Ulva) to 26.37 ind.g-1 (in Pterosiphonia) of alga-substratum weigth, and the population was distributed mainly in branched algae. It was determined three size classes in the population, within a range from 0.01 to 2.99mm of pereonits 1-4 length. Small amphipods prefer finely branched algae like Gymnogongrusand Pterosiphonia, whereas broad-thallii or less branched algae such as Sargassum, Pterocladia, Gelidium and Ulva harbour proporcionally high number of large individuais. The life cycle of Hyale media takes place wholly in the phytals and the species reproduces continually all year round: males, ovigerous females and juveniles are present every season. The highest femalc reproductive activity occurs in winter and the juveniles are more numerous in summer. The number of eggs inside the marsupium and the pereonites 1-4 length has a linear correlation (Y=-9,9682+12,0729X; r=0.8024).

Janete, Dubiaski-Silva; Setuko, Masunari.

199

Copper-induced calcium release from ER involves the activation of ryanodine-sensitive and IP3-sensitive channels in Ulva compressa  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The marine alga Ulva compressa (Chlorophyta) showed a triphasic release of intracellular calcium with maximal levels at 2, 3 and 12 h and a biphasic accumulation of intracellular hydrogen peroxide with peaks at 3 and 12 h when cultivated with copper excess. Intracellular hydrogen peroxide originated exclusively in organelles. In this work, we analyzed the intracellular origin of calcium release and the type of calcium channels activated in response to copper excess. U. compressa was treated w...

Gonza?lez, Alberto; Trebotich, Jovanka; Vergara, Eva; Medina, Cristo?bal; Morales, Bernardo; Moenne, Alejandra

2010-01-01

200

The influence of sediment cohesiveness on bioturbation effects due to Hydrobia ulvae on the initial erosion of intertidal sediments: A study combining flume and model approaches  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Laboratory experiments performed in a recirculating flume were designed to quantify the bioturbation influence of the mud snail Hydrobia ulvae, one of the most abundant deposit feeders on European intertidal mudflats. Variations in sediment moisture content that occur between bedforms in shore-normal, ridge and runnel systems of intertidal mudflats were added to the model definition. Sediment erosion thresholds, erosion rates and the microalgal pigment composition of resuspended material were...

Orvain, F.; Sauriau, Pierre-guy; Bacher, Cedric; Prineau, Michel

2006-01-01

 
 
 
 
201

The stoichiometry and antenna size of the two photosystems in marine green algae, Bryopsis maxima and Ulva pertusa, in relation to the light environment of their natural habitat.  

Science.gov (United States)

The stoichiometry and antenna sizes of the two photosystems in two marine green algae, Bryopsis maxima and Ulva pertusa, were investigated to examine whether the photosynthetic apparatus of the algae can be related to the light environment of their natural habitat. Bryopsis maxima and Ulva pertusa had chlorophyll (Chl) a/b ratios of 1.5 and 1.8, respectively, indicating large levels of Chl b, which absorbs blue-green light, relative to Chl a. The level of photosystem (PS) II was equivalent to that of PS I in Bryopsis maxima but lower than that of PS I in Ulva pertusa. Analysis of Q(A) photoreduction and P-700 photo-oxidation with green light revealed that >50% of PS II centres are non-functional in electron transport. Thus, the ratio of the functional PS II to PS I is only 0.46 in Bryopsis maxima and 0.35 in Ulva pertusa. Light-response curves of electron transport also provided evidence that PS I had a larger light-harvesting capacity than did the functional PS II. Thus, there was a large imbalance in the light absorption between the two photosystems, with PS I showing a larger total light-harvesting capacity than PS II. Furthermore, as judged from the measurements of low temperature fluorescence spectra, the light energy absorbed by Chl b was efficiently transferred to PS I in both algae. Based on the above results, it is hypothesized that marine green algae require a higher ATP:NADPH ratio than do terrestrial plants to grow and survive under a coastal environment. PMID:15797939

Yamazaki, Jun-Ya; Suzuki, Takahisa; Maruta, Emiko; Kamimura, Yasumaro

2005-06-01

202

Allelopathic effects of Ulva pertusa, Corallina pilulifera and Sargassum thunbergii on the growth of the dinoflagellates Heterosigma akashiwo and Alexandrium tamarense  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The allelopathic effects of fresh tissue, dry powder and aqueous extracts of three macroalgae, Ulva pertusa, Corallina pilulifera and Sargassum thunbergii, on the growth of the dinoflagellates Heterosigma akashiwo and Alexandrium tamarense were evaluated using coexistence culture systems in which concentrations of the three macroalga were varied. The results of the coexistence assay showed that the growth of the two microalgae was strongly inhibited by using fresh tissue, dry powder and aqueo...

Wang, Renjun; Xiao, Hui; Zhang, Peiyu; Qu, Liang; Cai, Hengjiang; Tang, Xuexi

2007-01-01

203

Efeito da substituição da farinha de peixe por farinha de algas Gracilaria sp. e Ulva rigida no crescimento e nos parâmetros metabólicos da dourada (Sparus aurata)  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Este estudo foi conduzido com o intuito de investigar o efeito da substituição de farinha de peixe por farinha de algas Gracilaria sp. e Ulva rigida no crescimento e nos parâmetros metabólicos da dourada (Sparus aurata). Seis dietas experimentais foram testadas com farinha de alga a diferentes percentagens de substituição: 5, 15 e 25% e uma dieta controlo sem incorporação de algas. A experiência foi levada a cabo num sistema aberto de circulação usando exemplares ima...

Batista, Sara Isabel Mendes

2008-01-01

204

Accumulation of heavy metals (Co, Cr, Cu, Mn, Zn) in the freshwater alga Ulva type, sediments and water of the Wielkopolska region, Poland.  

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The concentration of five trace elements: cobalt (Co), chrome (Cr), copper (Cu), manganese (Mn) and zinc (Zn) was determined in the Ulva thalli, in the water and sediment collection from several inland sites (lakes, stream and river) from the Wielkopolska region during summer 2010. The multielemental analysis of the heavy metal concentration was carried out with the use of ICP-OES method. The aim of this study was to determine the role of tubular forms as biomonitoring species....

Rybak, Andrzej; Messyasz, Beata; Pikosz, Marta; Szendzina, Lidia; ?e?ska, Bogus?awa

2013-01-01

205

Chemical and biological evaluation of the nutritive value of Algerian green seaweed Ulva lactuca using in vitro gas production technique fior ruminant animals  

Digital Repository Infrastructure Vision for European Research (DRIVER)

This study aimed to determine the nutritive value of seaweed Ulva lactuca collected from the Algerian coast by estimation of its chemical composition and fermentation characteristics, comparatively to vetch-oat hay (control), using in vitro gas production technique. Seaweed and control were incubated with rumen liquor taken from fistulated and non lactating cows. Gas production was recorded at: 2, 4, 6, 8, 12, 24, 48 and 72h. The in vitro rumen fermentation parameters were measured after 24h ...

Zitouni, Hind; Arhab, Rabah; Boudry, Christelle; Bousseboua, Hace?ne; Beckers, Yves

2014-01-01

206

Oxidative stress and enzymatic scavenging of superoxide radicals induced by solar UV-B radiation in Ulva canopies from southern Spain  

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The generation of reactive oxygen species (ROS) and scavenging of the superoxide radical by superoxide dismutase (SOD) was studied in mat-like canopies of the green macroalga Ulva rotundata Bliding in a tidal brine pond system in southern Spain. Artificial canopies were covered with different cut-off filters, generating different radiation conditions. ROS and SOD were assessed after three days of exposure. ROS induced lipid peroxidation depended on the position of individual thalli within the...

Bischof, K.; Janknegt, P. J.; Buma, A. G. J.; Rijstenbil, J. W.; Peralta, G.; Breeman, A. M.

2003-01-01

207

The influence of surface energy on the wetting behaviour of the spore adhesive of the marine alga Ulva linza (synonym Enteromorpha linza)  

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The environmental scanning electron microscope has been used to image the adhesive pads secreted by zoospores of the marine alga Ulva linza as they settle on a range of self-assembled and grafted monolayers of different wettability, under natural, hydrated conditions. Results reveal that the diameter of the adhesive pad is strongly influenced by surface wettability, the adhesive spreading more (i.e. wetting the surface better) on the more hydrophilic surfaces. This is in direct contrast to pr...

Callow, J. A.; Callow, M. E.; Ista, L. K.; Lopez, G.; Chaudhury, M. K.

2005-01-01

208

Zonação de comunidade bêntica do entremarés em molhes sob diferente hidrodinamismo na costa norte do estado do Rio de Janeiro, Brasil / Zonation of intertidal benthic communities on breakwaters of different hydrodynamics in the north coast of the state of Rio de Janeiro, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O presente estudo pretende comparar a distribuição vertical da comunidade bêntica na zona entremarés em dois locais compostos por matacões graníticos caracterizados por hidrodinâmica distinta, reflexo da diferença na orientação dos molhes nas praias do Farol de São Tomé (Píer) e na Barra do Furado ( [...] Barra), norte do Estado do Rio de Janeiro. Quadrados de 400 cm² foram sobrepostos ao longo de três perfis verticais de ambos os sítios e amostrados através do método de fotoquadrats, desde o nível 0,2 m da maré até o limite superior de Littorina spp. O limite superior dos organismos marinhos foi ampliado na Barra (3,8 m) em relação ao Píer (2,2 m). Quanto à composição taxonômica, nove espécies foram comuns. Chaetomorpha sp., Chondracanthus teedii (Mertens ex Roth) Fredericq e Grateloupia sp. foram exclusivas na Barra, enquanto Tetraclita stalactifera (Lamarck, 1818), Fissurella clench, Gracilaria domingensis (Kützing) Sonder ex Dickie e Hypnea musciformis (Wulfen in Jacqu.) Lamouroux ocorreram somente no Píer. Em ambos os locais, a riqueza e a diversidade de espécies foram superiores nos quadrados intermediários. Os maiores valores foram registrados na Barra. As maiores diferenças nos agrupamentos entre faixas equivalentes de locais distintos ocorreram na faixa eulitorânea superior, seguida pela faixa eulitorânea inferior e franja sublitorânea. Apenas a orla litorânea não revelou diferença significativa entre os locais, mas uma maior extensão desta franja e da faixa eulitorânea superior era bastante evidente. As demais faixas na Barra do Furado foram caracterizadas em grande parte por espécies típicas de ambientes mais expostos como Chaetomorpha sp. na faixa eulitorânea superior e Perna perna (Linnaeus, 1758) na eulitorânea inferior, além de C. teedii e Ulva fasciata Delile, 1813 na franja sublitorânea. No Píer, as diferentes faixas apresentavam distribuição eqüitativa, refletindo um ambiente menos estressante. As diferenças observadas na distribuição vertical dos organismos bênticos, principalmente na extensão das faixas superiores, evidenciam condições de exposição a ondas variáveis. Abstract in english The present study aims to compare the vertical distribution of intertidal benthic communities in two sites composed by granitic boulders with distint hydrodynamics due to different wave swells at Farol de São Tomé (Pier) and Barra do Furado beaches (Barra), both in northern state of Rio de Janeiro. [...] Quadrats of 400 cm² were overlapped along three vertical profiles on each site and were sampled by the photoquadrat method from 0.2 m of the tide level to the upper limit of Littorina spp. The upper limit of the marine organisms was higher at Barra site (3.8 m) than at Píer site (2.2 m). Nine species were common to both sites. Chaetomorpha sp., Chondracanthus teedii (Mertens ex Roth) Fredericq, and Grateloupia sp. were unique to Barra, whereas Tetraclita stalactifera (Lamarck, 1818), Fissurella clench, Gracilaria domingensis (Kützing) Sonder ex Dickie, and Hypnea musciformis (Wulfen in Jacqu.) Lamouroux occurred only at Píer boulders. On both sites species richness and diversity were superior at the intermediate quadrats of the intertidal zone. The highest values were recorded at Barra. The most pronounced assemblage differences between equivalent areas of either site occurred on the lower upper eulittoral band followed by the lower eulittoral and the sub-littoral fringe, respectively. The littoral fringe assemblage was the only one that did not show significant differences among the studied sites, yet a larger range of this fringe and the upper eulittoral band at Barra was quite evident. This site was mostly characterized by species of more exposed areas, such as Chaetomorpha sp. and Perna perna (Linnaeus, 1758) on upper and lower eulittoral bands, and by C. teedii and Ulva fasciata Delile, 1813 on the sub-littoral fringe. At Píer, the intertidal zone showed an even distr

Bruno P., Masi; Ilana R., Zalmon.

209

Zonação de comunidade bêntica do entremarés em molhes sob diferente hidrodinamismo na costa norte do estado do Rio de Janeiro, Brasil Zonation of intertidal benthic communities on breakwaters of different hydrodynamics in the north coast of the state of Rio de Janeiro, Brazil  

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Full Text Available O presente estudo pretende comparar a distribuição vertical da comunidade bêntica na zona entremarés em dois locais compostos por matacões graníticos caracterizados por hidrodinâmica distinta, reflexo da diferença na orientação dos molhes nas praias do Farol de São Tomé (Píer e na Barra do Furado (Barra, norte do Estado do Rio de Janeiro. Quadrados de 400 cm² foram sobrepostos ao longo de três perfis verticais de ambos os sítios e amostrados através do método de fotoquadrats, desde o nível 0,2 m da maré até o limite superior de Littorina spp. O limite superior dos organismos marinhos foi ampliado na Barra (3,8 m em relação ao Píer (2,2 m. Quanto à composição taxonômica, nove espécies foram comuns. Chaetomorpha sp., Chondracanthus teedii (Mertens ex Roth Fredericq e Grateloupia sp. foram exclusivas na Barra, enquanto Tetraclita stalactifera (Lamarck, 1818, Fissurella clench, Gracilaria domingensis (Kützing Sonder ex Dickie e Hypnea musciformis (Wulfen in Jacqu. Lamouroux ocorreram somente no Píer. Em ambos os locais, a riqueza e a diversidade de espécies foram superiores nos quadrados intermediários. Os maiores valores foram registrados na Barra. As maiores diferenças nos agrupamentos entre faixas equivalentes de locais distintos ocorreram na faixa eulitorânea superior, seguida pela faixa eulitorânea inferior e franja sublitorânea. Apenas a orla litorânea não revelou diferença significativa entre os locais, mas uma maior extensão desta franja e da faixa eulitorânea superior era bastante evidente. As demais faixas na Barra do Furado foram caracterizadas em grande parte por espécies típicas de ambientes mais expostos como Chaetomorpha sp. na faixa eulitorânea superior e Perna perna (Linnaeus, 1758 na eulitorânea inferior, além de C. teedii e Ulva fasciata Delile, 1813 na franja sublitorânea. No Píer, as diferentes faixas apresentavam distribuição eqüitativa, refletindo um ambiente menos estressante. As diferenças observadas na distribuição vertical dos organismos bênticos, principalmente na extensão das faixas superiores, evidenciam condições de exposição a ondas variáveis.The present study aims to compare the vertical distribution of intertidal benthic communities in two sites composed by granitic boulders with distint hydrodynamics due to different wave swells at Farol de São Tomé (Pier and Barra do Furado beaches (Barra, both in northern state of Rio de Janeiro. Quadrats of 400 cm² were overlapped along three vertical profiles on each site and were sampled by the photoquadrat method from 0.2 m of the tide level to the upper limit of Littorina spp. The upper limit of the marine organisms was higher at Barra site (3.8 m than at Píer site (2.2 m. Nine species were common to both sites. Chaetomorpha sp., Chondracanthus teedii (Mertens ex Roth Fredericq, and Grateloupia sp. were unique to Barra, whereas Tetraclita stalactifera (Lamarck, 1818, Fissurella clench, Gracilaria domingensis (Kützing Sonder ex Dickie, and Hypnea musciformis (Wulfen in Jacqu. Lamouroux occurred only at Píer boulders. On both sites species richness and diversity were superior at the intermediate quadrats of the intertidal zone. The highest values were recorded at Barra. The most pronounced assemblage differences between equivalent areas of either site occurred on the lower upper eulittoral band followed by the lower eulittoral and the sub-littoral fringe, respectively. The littoral fringe assemblage was the only one that did not show significant differences among the studied sites, yet a larger range of this fringe and the upper eulittoral band at Barra was quite evident. This site was mostly characterized by species of more exposed areas, such as Chaetomorpha sp. and Perna perna (Linnaeus, 1758 on upper and lower eulittoral bands, and by C. teedii and Ulva fasciata Delile, 1813 on the sub-littoral fringe. At Píer, the intertidal zone showed an even distribution, reflecting a less stressful environment. The differences on vertical distribution of the local benthic,

Bruno P. Masi

2008-12-01

210

Zonação de comunidade bêntica do entremarés em molhes sob diferente hidrodinamismo na costa norte do estado do Rio de Janeiro, Brasil / Zonation of intertidal benthic communities on breakwaters of different hydrodynamics in the north coast of the state of Rio de Janeiro, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O presente estudo pretende comparar a distribuição vertical da comunidade bêntica na zona entremarés em dois locais compostos por matacões graníticos caracterizados por hidrodinâmica distinta, reflexo da diferença na orientação dos molhes nas praias do Farol de São Tomé (Píer) e na Barra do Furado ( [...] Barra), norte do Estado do Rio de Janeiro. Quadrados de 400 cm² foram sobrepostos ao longo de três perfis verticais de ambos os sítios e amostrados através do método de fotoquadrats, desde o nível 0,2 m da maré até o limite superior de Littorina spp. O limite superior dos organismos marinhos foi ampliado na Barra (3,8 m) em relação ao Píer (2,2 m). Quanto à composição taxonômica, nove espécies foram comuns. Chaetomorpha sp., Chondracanthus teedii (Mertens ex Roth) Fredericq e Grateloupia sp. foram exclusivas na Barra, enquanto Tetraclita stalactifera (Lamarck, 1818), Fissurella clench, Gracilaria domingensis (Kützing) Sonder ex Dickie e Hypnea musciformis (Wulfen in Jacqu.) Lamouroux ocorreram somente no Píer. Em ambos os locais, a riqueza e a diversidade de espécies foram superiores nos quadrados intermediários. Os maiores valores foram registrados na Barra. As maiores diferenças nos agrupamentos entre faixas equivalentes de locais distintos ocorreram na faixa eulitorânea superior, seguida pela faixa eulitorânea inferior e franja sublitorânea. Apenas a orla litorânea não revelou diferença significativa entre os locais, mas uma maior extensão desta franja e da faixa eulitorânea superior era bastante evidente. As demais faixas na Barra do Furado foram caracterizadas em grande parte por espécies típicas de ambientes mais expostos como Chaetomorpha sp. na faixa eulitorânea superior e Perna perna (Linnaeus, 1758) na eulitorânea inferior, além de C. teedii e Ulva fasciata Delile, 1813 na franja sublitorânea. No Píer, as diferentes faixas apresentavam distribuição eqüitativa, refletindo um ambiente menos estressante. As diferenças observadas na distribuição vertical dos organismos bênticos, principalmente na extensão das faixas superiores, evidenciam condições de exposição a ondas variáveis. Abstract in english The present study aims to compare the vertical distribution of intertidal benthic communities in two sites composed by granitic boulders with distint hydrodynamics due to different wave swells at Farol de São Tomé (Pier) and Barra do Furado beaches (Barra), both in northern state of Rio de Janeiro. [...] Quadrats of 400 cm² were overlapped along three vertical profiles on each site and were sampled by the photoquadrat method from 0.2 m of the tide level to the upper limit of Littorina spp. The upper limit of the marine organisms was higher at Barra site (3.8 m) than at Píer site (2.2 m). Nine species were common to both sites. Chaetomorpha sp., Chondracanthus teedii (Mertens ex Roth) Fredericq, and Grateloupia sp. were unique to Barra, whereas Tetraclita stalactifera (Lamarck, 1818), Fissurella clench, Gracilaria domingensis (Kützing) Sonder ex Dickie, and Hypnea musciformis (Wulfen in Jacqu.) Lamouroux occurred only at Píer boulders. On both sites species richness and diversity were superior at the intermediate quadrats of the intertidal zone. The highest values were recorded at Barra. The most pronounced assemblage differences between equivalent areas of either site occurred on the lower upper eulittoral band followed by the lower eulittoral and the sub-littoral fringe, respectively. The littoral fringe assemblage was the only one that did not show significant differences among the studied sites, yet a larger range of this fringe and the upper eulittoral band at Barra was quite evident. This site was mostly characterized by species of more exposed areas, such as Chaetomorpha sp. and Perna perna (Linnaeus, 1758) on upper and lower eulittoral bands, and by C. teedii and Ulva fasciata Delile, 1813 on the sub-littoral fringe. At Píer, the intertidal zone showed an even distr

Bruno P., Masi; Ilana R., Zalmon.

2008-12-01

211

Characterization of chlorophyll-protein complexes isolated from two marine green algae, Bryopsis maxima and Ulva pertusa, growing in the intertidal zone.  

Science.gov (United States)

Three Chl-protein complexes were isolated from thylakoid membranes of Bryopsis maxima and Ulva pertusa, marine green algae that inhabit the intertidal zone of the Pacific Ocean off the eastern coast of Japan by dodecyl-beta-D-maltoside polyacrylamide gel electrophoresis. The slowest-moving fractions showed low Chl a/b and Chl/P-700 ratios, indicating that this fraction corresponds to complexes in PS I, which is large in both algae. The intermediate and fastest-moving fractions showed the traits of PS II complexes, with some associated Chl a/b-protein complexes and LHC II, respectively. The spectral properties of the separated Chl-proteins were also determined. The absorption spectra showed a shallow shoulder at 540 nm derived from siphonaxanthin in Bryopsis maxima, but not in Ulva pertusa. The 77 K emission spectra showed a single peak in Bryopsis maxima and two peaks in Ulva pertusa. Besides the excitation spectra indicated that the excitation energy transfer to the PS I complexes differed quite a lot higher plants. This suggested that the mechanisms of energy transfer in both of these algae differ from those of higher plants. Considering the light environment of this coastal area, the large size of the antennae of PS I complexes implies that the antennae are arranged so as to balance light absorption between the two photosystems. In addition, we discuss the relationships among the photosystem stoichiometry, the energy transfer, and the distribution between the two photosystems. PMID:16729200

Yamazaki, Jun-ya; Kozu, Arisu; Fukunaga, Yuko

2006-07-01

212

Reduction of nitrogen in the excretion on Japanese flounder using Ulva and Capitellid; Anaaosa to itogokai ni yoru hirame haisetsubutsuchu no chisso shori  

Energy Technology Data Exchange (ETDEWEB)

To develop the culture residue treatment technique using aquatic organisms, the ammonia and nitrate uptake rates of seaweed Ulva and the nitrogen reduction rate of polychaeta annelid Captella sp. with organic sediment predaceous ability were examined in the excretion of Japanese flounder. Nitrogen uptake rate of Ulva was affected by water temperature. It was highest at 20degC, followed at 15degC and 25degC in the order. It was not affected by light intensity between 1500 and 6000 lux. Ammonia and nitrate uptake rates by Ulva were estimated to be 28.2 and 14.6 {mu}g-N/g/h at 20degC under 3000 lux, respectively. Proportion of feces excreted from Capitellid to ingested sediments was 0.38. At 25degC, Capitellid population of one thousand individuals ingested-N at the rate of 24 mg-N/day, and excreted the feces-N of Capitellid at the rate of 7 mg-N/day. About 70% of nitrogen in the sediment was reduced through this process. 15 refs., 9 figs., 13 tabs.

Honda, H.; Kikuchi, K.; Sakaguchi, I. [Central Research Institute of Electric Power Industry, Tokyo (Japan)

1997-03-01

213

INFLUÊNCIA DO CANAL MARINHO DA ILHA DE VITÓRIA-ES SOBRE A BIOMASSA DE ALGAS DO GÊNERO Ulva sp. (CHLOROPHYTA, ULVALES  

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Full Text Available As informações a respeito do tamanho da biomassa de Ulva sp aqui apresentadas têm por objetivo contribuirpara o conhecimento deste gênero, focando o fornecimento de subsídios para estratégias de conservação doecossistema aquático. O objetivo deste estudo foi observar se ocorre diferença na biomassa de Ulva sp emdiferentes pontos localizados próximos a um canal com poluição orgânica de esgoto. As condições doambiente ou fatores abióticos, como a poluição, induzem uma serie de respostas fisiológicas em organismos,que determinam se o ambiente físico é habitável ou não . Foram escolhidos 3 pontos alinhados em relação aoponto de poluição, de modo que a incidência de poluição seria maior no primeiro do que no segundo e maiorno segundo do que no terceiro (200 metros equidistantes. Cinco quadrantes foram retirados de cada ponto,triados e pesados. No presente estudo foi evidenciado uma resposta fisiológica quanto ao crescimento da algaverde do gênero Ulva conhecida como alface-do-mar. Observa-se que os pontos dois e três não foram tãoafetados quanto o ponto um, este localizado mais próximo da poluição respondeu tendo metade do pesoencontrado nos outros pontos.

Vitor Araújo-Lima

2012-04-01

214

Influence of combined changes in salinity and copper modulation on growth and copper uptake in the tropical green macroalga Ulva reticulata  

Science.gov (United States)

The influence of salinity on growth and Cu uptake in the green macroalga Ulva reticulata collected from the intertidal area in the Western Indian Ocean was studied under controlled laboratory conditions. Exposure concentrations ranged from 5 to 500 ?g Cu l -1 at five salinities (ranging 20-40). The accumulation of Cu increased with decreasing salinity, so that the uptake at 500 ?g Cu l -1 was approximately 2.7, 2.4 and 2.0 times higher at salinities of 20, 25, and 30 respectively, than uptake at salinity of 35, and with uptake being lowest at salinity of 40. Ulva maintained a positive growth rate over the whole salinity range (20-40), with highest rates at salinity of 35. When exposing to Cu at low salinities (20 and 25), the growth rate of Ulva was strongly inhibited suggesting an increase in toxicity of Cu with decreasing salinity. EC50 and NOEC increased with increase in salinity, implying a reduced Cu toxicity at high salinities. It was concluded that salinity needs to be considered when using macroalgae, such as U. reticulata, as a bioindicator of heavy metals in areas with heavy rainfall, underground fresh water intrusion or in estuaries, as they might accumulate more metals and be more negatively affected.

Mamboya, Florence; Lyimo, Thomas J.; Landberg, Tommy; Björk, Mats

2009-09-01

215

Nitrous oxide emission from Ulva lactuca incubated in batch cultures is stimulated by nitrite, nitrate and light  

DEFF Research Database (Denmark)

Biomass yields from some species of macroalgae exceed the yields in traditional terrestrial production systems. This renewable carbon source possesses a potential for energy purposes and thus reduction in fossil fuel carbon dioxide (CO2) emissions. Previous experiments have indicated that nitrous oxide (N2O) may be produced by green algae. We investigated the N2O emissions in the green alga Ulva lactuca. Significant N2O emissions, along with CO2 uptake, were demonstrated from vital U. lactuca material from different natural populations incubated in the laboratory with nitrite (NO2?) and nitrate (NO3?) and at a light intensity of 225?molphotonsm?2s?1. No emission of N2O was observed in darkness. The N2O emission increased in a Michaelis–Menten characteristic manner with increasing concentrations of both NO3? and NO2?. The light dependency indicated that the N2O emission was related to algal photosynthesis, and not bacterial activity. As algal NO3? reductase (NR) converts NO3? to NO2? in light, and N2O emissionwas observed from both NO3? and NO2?, it is proposed that NO2? reductase (NiR) activity may have generated the observed N2O, however the mechanism needs further investigation. This apparent N2O production by algae emphasizes the need for experiments under natural conditions in order to evaluate potential greenhouse gas balances associated with large-scale productions for energy purposes.

Albert, Kristian Rost; Ambus, Per

2013-01-01

216

[Effects of Ulva pertusa and Gracilaria lemaneiformis on growth of Heterosigma akashiwo (Raphidophyceae) in co-culture].  

Science.gov (United States)

We studied the effects of fresh tissue and culture medium filtrate of two species of macroalgae, Ulva pertusa (Chlorophyta) and Gracilaria lemaneiformis (Rhodophyta) on growth of Heterosigma akashiwo (Raphidophyceae) in co-culture. Both U. pertusa and G. lemaneiformis, and especially their fresh tissues, significantly impede the growth of H. akashiwo. Carbonate limitations and the presence of environment bacteria are not necessary for the negative effects of macroalgal on H. akashiwo. The simultaneous nutrient assays show that nitrate and phosphate are almost exhausted in the G. lemaneiformis co-culture system, but remain at acceptable levels in the U. pertusa system, when all cells of H. akashiwo are completely dead. When f/2 medium is supplied daily to G. lemaneiformis culture, the growth of H. akashiwo is greatly inhibited but not completely terminated. Furthermore, different amounts of fresh seaweed tissue, and culture medium filtrate prepared from different macroalgal concentrations are analyzed to determine their effects on the growth of H. akashiwo. The results show a positive correlation between the initial macroalgal concentration and the negative effects they exert on the co-cultured microalgae. Results suggest that the allelopathic effects of U. pertusa may be essential for negative effects on H. akashiwo; however, the combined roles of allelopathy and nutrient competition may be responsible for the negative effect of G. lemaneiformis the release of allelochemicals by U. pertusa. PMID:16686183

Wang, You; Yu, Zhi-ming; Song, Xiu-xian; Zhang, Shan-dong

2006-02-01

217

Isolation and characterization of a single-copy actin gene from a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta).  

Science.gov (United States)

We constructed a cDNA library from sterile Ulva pertusa (Ulvales, Chlorophyta), and isolated and characterized a full-length cDNA clone encoding actin. The actin (ACT) cDNA consisted of 1487 nucleotides (nt) and had an open reading frame (ORF) encoding a polypeptide of 377 amino acid (AA) residues. The ACT gene had one intron in the 5'-untranslated region and three introns in the coding region. Transcription started 26 nt downstream of the putative TATA box. A potential polyadenylation signal, TGTAG, was located 100 nt downstream of the terminator codon, TAG. Amino acid alignment with actins from various algae and land plants showed that sterile U. pertusa actin was more similar to actins from Chlorophyta, Phaeophyta, Euglenophyta, and higher plants (over 76.9%) than to actins from Rhodophyta. Southern blot analysis indicated that the sterile U. pertusa genome has only a single actin-encoding gene. Thalli grown on a 12D/12L photoperiod increased in surface area some two-fold over 24 h regardless of the nutritional conditions. The growth rate of thalli during the light period was significantly higher than that during the dark period. Northern hybridization indicated that the expression of actin mRNA was induced and repressed by the light and dark treatments, respectively. These results suggest that the U. pertusa cell division cycle has a periodicity of 24 h and that the ACT gene is highly transcribed during cell growth and development in the light period. PMID:15256264

Kakinuma, Makoto; Coury, Daniel A; Inagaki, Eri; Itoh, Sayaka; Yoshiura, Yasutoshi; Amano, Hideomi

2004-06-01

218

Application of Ulva lactuca and Systoceira stricta algae-based activated carbons to hazardous cationic dyes removal from industrial effluents.  

Science.gov (United States)

Marine algae Ulva lactuca (ULV-AC) and Systoceira stricta (SYS-AC) based activated carbons were investigated as potential adsorbents for the removal of hazardous cationic dyes. Both algae were surface oxidised by phosphoric acid for 2 and subsequently air activated at 600 °C for 3 h. Dyes adsorption parameters such as solution pH, contact time, carbon dosage, temperature and ionic strength were measured in batch experiments. Adsorption capacities of 400 and 526 mg/g for Malachite green and Safranine O by the SYS-AC and ULV-AC respectively were significantly enhanced by the chemical treatments. Model equations such as Langmuir, Freundlich and Temkin isotherms were used to analyse the adsorption equilibrium data and the best fits to the experimental data were provided by the first two isotherm models. BET, FT-IR, iodine number and methylene blue index determination were also performed to characterize the adsorbents. To describe the adsorption mechanism, kinetic models such as pseudo-second-order and the intra particle diffusion were applied. Thermodynamic analysis of the adsorption processes of both dyes confirms their spontaneity and endothermicity. Increasing solution ionic strength increased significantly the adsorption of Safranine O. This study shows that surface modified algae can be an alternative to the commercially available adsorbents for dyes removal from liquid effluents. PMID:23597681

Salima, Attouti; Benaouda, Bestani; Noureddine, Benderdouche; Duclaux, Laurent

2013-06-15

219

Evidence for abrasion and enhanced growth of Ulva lactuca L. in the presence of colliery waste particles  

Energy Technology Data Exchange (ETDEWEB)

Previous studies have highlighted a reduction in occurrence and biomass of Ulva lactuca L. (Chlorophyceae) on shores where inputs of colliery waste occur. It was postulated that this was owing to an abrasive effect of colliery waste on macroalgal fronds. To test this, individual U. lactuca plants were exposed to colliery waste (three different grain size categories: {lt} 500 {mu}m, 500-2000 {mu}m, and 0-2000 {mu}m) in both shaken (turbulent) and still conditions in the laboratory. Over an 8-day period, U. lactuca plants lost weight when colliery waste was present and gained weight when no colliery waste was present. The results suggest that `large` grains of colliery waste act as a physical abrading agent on macroalgae when in turbulent conditions, and may be responsible for lowering of species richness of macroalgae where colliery waste inputs occur. However, by contrast, colliery waste in still conditions promotes the growth of U. lactuca, suggesting that, for example, rock pool flora may benefit from its presence.

Hyslop, B.T.; Davies, M.S. [University of Sunderland, Sunderland (United Kingdom). Northumbrian Water Ecology Centre

1998-12-01

220

Effects of five antifouling biocides on settlement and growth of zoospores from the marine macroalga Ulva lactuca L.  

Science.gov (United States)

Antifouling biocides are found in the marine ecosystem were they can affect non-target organisms. In this study the effects of five antifouling biocides on the settlement and growth of Ulva lactuca zoospores were investigated. The biocides investigated were copper (Cu(2+)), 4,5-dichloro-2-n-octyl-3(2H)-isothiazolone (DCOIT), triphenylborane pyridine (TPBP), tolylfluanid and medetomidine. Full concentration-response curves where determined for each compound. EC50 values were determined for copper, DCOIT, TPBP and tolylfluanid, all of which inhibited settlement and growth in a concentration dependent manner with the following toxicity ranking; tolylfluanid (EC50 80 nmol L(-1)) ~ DCOIT (EC50 83 nmol L(-1)) > TPBP (EC50 400 nmol L(-1)) > Cu(2+) (EC50 2,000 nmol L(-1)). Medetomidine inhibited settlement and growth only at the extreme concentration of 100,000 nmol L(-1) (93% effect). The low toxicity is possibly a consequence of a lack of receptors that medetomidine can bind to in the U. lactuca zoospores. PMID:23846394

Wendt, Ida; Arrhenius, Åsa; Backhaus, Thomas; Hilvarsson, Annelie; Holm, Kristina; Langford, Katherine; Tunovic, Timur; Blanck, Hans

2013-10-01

 
 
 
 
221

The consumption of algae, Ulva lactuca, by the snail, Littorina littorea, in relation to colliery waste contamination  

Energy Technology Data Exchange (ETDEWEB)

Colliery waste is a persistent contaminant of intertidal areas in north-east England where it can reduce biodiversity. In an attempt to understand the ways in which colliery waste exerts its effects, the authors tested how a common molluscan grazer (Littorina littorea) from a range of contaminated and uncontaminated sites reacts to a common macroalgal component of its diet (Ulva lactuca) from the same range of sites. It is hypothesised that algae that are exposed to colliery waste are rejected (eaten less) by snails than those algae that are not so exposed. It is also hypothesised that snails exposed to colliery waste would prefer (eat more) algae from contaminated sites and those animals not so exposed would prefer algae from uncontaminated sites. No differences in feeding were observed in relation to these hypotheses and it is concluded that the contamination of shores by colliery waste does not affect the feeding of L. littorea on U. lactuca. However, snails from contaminated sites ate significantly more algae than those from uncontaminated sites. This may be because of differential digestion or metabolism of the algae or because of a paucity of suitable macroalgae at contaminated sites, snails feeding heavily on the rare U. lactuca when it is encountered.

Davies, M.S.; Falconer, F. [University of Sunderland, Durham (United Kingdom). Centre of Ecology

2001-07-01

222

Effects of culture conditions on the growth and reproduction of Gut Weed, Ulva intestinalis Linnaeus (Ulvales, Chlorophyta  

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Full Text Available In vitro cultivation of Gut Weed, Ulva intestinalis Linnaeus, was experimentally studied to support its near optimalfarming, with potential impact in Thailand on its direct use as human food or its co-cultures in shrimp farming.Germling clusters (2 weeks old and 7.50±2.98 mm long were seeded into 500 mL flasks and biomass growth rateoptimized with respect to the main controllable factors; seedling density, salinity, light intensity, and temperature. These factorswere assumed to each have an optimal value independent of the others, and the factors were optimized one at a time. Themaximum growth at three to four weeks of cultivation was obtained at the factor levels of 0.05 gL-1, 20 ppt, 80 ?mol photonm-2s-1 and 25°C. Early zoosporangia were obtained from 2nd to 4th weeks. The relative growth rate ranged from 9.47 to 22.18 %day-1, and only asexual reproduction of U. intestinalis was observed under these culture conditions.

Rapeeporn Ruangchuay

2012-11-01

223

Metabolic regulation of ammonium uptake by Ulva rigida (Chlorophyta): A compartmental analysis of the rate-limiting step for uptake  

International Nuclear Information System (INIS)

Non-linear time courses of ammonium (NH4+) depletion from the medium and internal accumulation of soluble nitrogen (N) in macroalgae imply that the rate-limiting step for ammonium uptake changes over time. We tested this hypothesis by measuring the time course of N accumulation in N-limited Ulva rigida C. Agardh. Total uptake was measured as removal of NH4+ from medium. Rates for the component processes (transport of NH4+ across the membrane = Rt, assimilation of tissue NH4+ into soluble N compounds = Ra, and incorporation of soluble N compounds into macromolecules = Ri) were determined by measuring the rate of labelling of the major tissue N pools after the addition of 15N-ammonium. The results indicate that nitrogen-specific rates (mass N taken up/mass N present/unit time) are ranked in the order of Rt > Ra > Ri. Absolute uptake rates (?mol N·mg dry wt-1·h-1) showed a different relationship. Membrane transport appears to be inhibited when NH4+ accumulates in the tissue. Maximum uptake rates occur when assimilation of NH4+ into soluble N compounds begins. Assimilation of NH4+ into soluble N compounds was initially faster than incorporation of soluble N compounds into macromolecules. Implications of rate limitations ules. Implications of rate limitations caused by differences in maximal rates and maximal pool sizes are discussed

224

Antioxidant potential of Ulva rigida extracts: protection of HeLa cells against H?O? cytotoxicity.  

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The rising demand for natural antioxidants instead of synthetic materials, especially in biomedical applications, has led to increased interest in the search for bioactive compounds with potent antioxidant activity. In the present study, we tested the antioxidant effect of both a crude extract and an ethanol precipitate of Ulva rigida in HeLa cells exposed to hydrogen peroxide (H?O?). HeLa cells treated with H?O? (1 mmol l?¹ for 3 h) exhibited significant damage to their morphology, a significant decrease in cell survival, and a remarkable leakage of lactate dehydrogenase (LDH). However, the co-exposure of cells to H?O? and the crude extract or ethanol precipitate of U. rigida induced fewer morphological cytotoxic effects, a significant increase in cell viability, and a significant decrease in LDH release. Biochemical studies have demonstrated that U. rigida extracts have a strong radicalscavenging activity and contain protein, sugar, and phenolic content. The overall results suggest that U. rigida extracts protect HeLa cells from death induced by oxidative stress, and it is likely that these effects are related to the phenolic, protein, and polysaccharide compounds contained in this alga. Hence, U. rigida can be used to treat diseases ascribed to oxidative disorders. PMID:24088791

Mezghani, Sana; Bourguiba, Ines; Hfaiedh, Imen; Amri, Mohamed

2013-09-01

225

Identification of toxicity variations in a stream affected by industrial effluents using Daphnia magna and Ulva pertusa.  

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A comprehensive toxicity monitoring study from August to October 2011 using Daphnia magna and Ulva pertusa was conducted to identify the cause of toxicity in a stream receiving industrial effluents (IEs) from a textile and leather products manufacturing complex. Acute toxicity toward both species was observed consistently in IE, which influenced toxicity of downstream (DS) water. A toxicity identification evaluation (TIE) confirmed that both Cu and Zn were key toxicants in the IE, and that the calculated toxicity based on Cu and Zn concentrations well simulated the variation in the observed toxicity (r(2)=0.9216 and 0.7256 for D. magna and U. pertusa, respectively). In particular, U. pertusa was sensitive enough to detect acute toxicity in DS and was useful to identify Zn as a key toxicant. Activities of catalase, superoxide dismutase, glutathione peroxidase, glutathione S-transferase, and malondialdehyde were induced significantly in D. magna, although acute toxicity was not observed. In addition, higher levels of antioxidant enzymes were expressed in DS than upstream waters, likely due to the Cu and Zn from IE. Overall, TIE procedures with a battery of bioassays were effective for identifying the cause of lethal and sub-lethal toxicity in effluent and stream water. PMID:23892313

Yoo, Jisu; Ahn, Byeongyong; Oh, Jeong-Ju; Han, Taejun; Kim, Woo-Keun; Kim, Sanghoon; Jung, Jinho

2013-09-15

226

The Influence of Charged Xerogel Side Chains on the Settlement and Adhesion of Ectocarpus crouaniorum and Ulva linza  

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A series of five xerogel coatings were prepared to evaluate the influence of charged surface moieties on the settlement and adhesion strength of Ectocarpus crouaniorum and Ulva linza. The coatings were prepared from mixtures of 3-(N,N-dimethylaminopropyl)-trimethoxysilane (DMAP), N-methylaminopropyl trimethoxysilane (MAP), 3-aminopropyl triethoxysilane (APTES), (3,3,3-trifluoropropyl) trimethoxysilane (TFP), and phenyltriethoxysilane (PH), and tetraethoxysilane (TEOS). Contact angle analysis and X-ray photoelectron spectroscopy were used to characterize the surface of each coating. After immersion in artificial seawater, the coatings possessed broadly similar surface energies (50+/-1 - 69+/-3 mN m-1) and a widely varying ability to have positively charged functionality at the surface. The settlement and percent removal assay for E. crouaniorum revealed a stronger adhesion of the alga to coatings possessing positively charged functionalities at the surface 1:9 DMAP/TEOS, 1:9 MAP/TEOS, and 1:9 AP/TEOS relative to the uncharged, non-basic 1:4 TFP/TEOS and 1:4 PH/TEOS coatings. The settlement and percent removal assay for U. linza also revealed stronger adhesion of sporelings to positively charged surfaces functionalities. These results suggest that charged moieties present at the surface is an important parameter to consider when developing coatings for foul-release purposes.

Gatley, Caitlyn M.

227

Influence of synthetic surfactants on the uptake of Pd, Cd and Pb by the marine macroalga, Ulva lactuca  

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Uptake of Pd, Cd and Pb by the marine macroalga, Ulva lactuca, has been studied in the presence of an anionic (sodium dodecyl sulphate, SDS), cationic (hexadecyltrimethylammonium bromide; HDTMA) and non-ionic (Triton X-100; TX) surfactant. Compared with the surfactant-free system, metal sorption was reduced in the presence of SDS or TX. Neither surfactant, however, had any measurable impact on cell membrane permeability, determined by leakage of dissolved free amino acids (DFAA), or on metal internalisation. We attribute these observations to the stabilisation of aqueous Cd and Pb by SDS and the shielding of otherwise amenable sorption sites by TX. Presence of HDTMA resulted in a reduction in the extent of both sorption and internalisation of all metals and a significant increase in the leakage of DFAA. Thus, by enhancing membrane permeability, HDTMA exerts the greatest influence on metal behaviour in the presence of U. lactuca. - Synthetic surfactants exert a significant impact on the uptake and internalisation of metals by a marine macroalga

228

Biosorption of uranium (VI) and thorium (IV) onto Ulva gigantea (Kuetzing) bliding. Discussion of adsorption isotherms, kinetics and thermodynamic  

International Nuclear Information System (INIS)

Ulva gigantea (Kuetzing) bliding (UGB) obtained from sea inlet of Izmir-Turkey has been studied as a biosorbent for removal of radioactive metals from water. In this study, unmodified UGB and modified UGB with glutaraldehyde (GUGB) characterized by FTIR spectroscopy were used as biosorbents for removal of U(VI) and Th(IV) ions from aqueous solution. Adsorption experiments performed under batch process with initial pH, contact time, adsorbent mass and temperature as variables. In order to determine the adsorption characteristics, Langmuir, Freundlich and Dubinin-Raduschkevich adsorption isotherms were applied to the adsorption data. Adsorption experiments showed that the adsorption isotherms correlated well with the Freundlich model. The sorption followed pseudo-second-order kinetics. The thermodynamic parameters such as variations of ?H0, ?G0 and ?S0 were estimated as a function of temperature. The thermodynamics of the adsorption of U(VI) and Th(IV) onto UGB and GUGB indicates that the spontaneous and exothermic nature of the process. The results showed that UGB and GUGB were potential for application in removal of U(VI) and Th(IV) from aqueous solution. (author)

229

Influence of synthetic surfactants on the uptake of Pd, Cd and Pb by the marine macroalga, Ulva lactuca  

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Uptake of Pd, Cd and Pb by the marine macroalga, Ulva lactuca, has been studied in the presence of an anionic (sodium dodecyl sulphate, SDS), cationic (hexadecyltrimethylammonium bromide; HDTMA) and non-ionic (Triton X-100; TX) surfactant. Compared with the surfactant-free system, metal sorption was reduced in the presence of SDS or TX. Neither surfactant, however, had any measurable impact on cell membrane permeability, determined by leakage of dissolved free amino acids (DFAA), or on metal internalisation. We attribute these observations to the stabilisation of aqueous Cd and Pb by SDS and the shielding of otherwise amenable sorption sites by TX. Presence of HDTMA resulted in a reduction in the extent of both sorption and internalisation of all metals and a significant increase in the leakage of DFAA. Thus, by enhancing membrane permeability, HDTMA exerts the greatest influence on metal behaviour in the presence of U. lactuca. - Synthetic surfactants exert a significant impact on the uptake and internalisation of metals by a marine macroalga.

Masakorala, Kanaji [School of Earth, Ocean and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Turner, Andrew [School of Earth, Ocean and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom)], E-mail: aturner@plymouth.ac.uk; Brown, Murray T. [School of Biological Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom)

2008-12-15

230

Shifts in bacterial and archaeal community structures during the batch biomethanation of Ulva biomass under mesophilic conditions.  

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Mesophilic biomethanation of Ulva biomass was performed in a batch bioreactor, and a high organic removal of 77% was obtained on the basis of chemical oxygen demand (COD) after a month of operation. The estimated methane yield was 0.43 ± 0.02 L CH4/g COD(removed) which is close to the theoretical methane potential. Transitions of bacterial and archaeal community structures, associated with process performance data, were investigated using a combination of molecular fingerprinting and biostatistical tools. During the operation, archaeal community structure had no significant changes while bacterial community structure shifted continuously and dynamically. The reactor completely stabilized volatile fatty acids (primarily acetate and propionate) accumulated from the acidogenesis phase, with Methanosaeta- and Methanolinea-related microbes respectively being the main aceticlastic and hydrogenotrophic methanogens. Methanolinea- and Syntrophobacter-related populations were likely the key members to form a syntrophic propionate-degrading consortium. A Methanolinea-related population was likely the dominant methane producer in the experimental reactor. PMID:25086435

Kim, Jaai; Jung, Heejung; Lee, Changsoo

2014-10-01

231

[Seasonal variations of metal contents (Cd, Cu, Fe, Mn and Zn) in seaweed Ulva lactuca from the coast of El Jadida city (Morocco)].  

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The quality of El Jadida Atlantic coastal water was monitored from April 1998 to March 1999 by measuring hydrological parameters (dissolved oxygen, suspended particulate matter, phosphates and nitrites) and using the seaweed Ulva lactuca as a quantitative bio-indicator of cadmium, copper, iron, manganese and zinc contamination. Metal content in seaweeds, collected every month from four stations characterized by the discharge of urban and industrial waste water, showed significant variations depending on the station and sampling period. However, the seaweed of El Jadida exhibited generally lower contents compared to those of similar species from other geographical areas. PMID:15212368

Kaimoussi, Aziz; Mouzdahir, Abdelkrim; Saih, Abdelkbir

2004-04-01

232

Production changes and distribution of ulva rigida in Venice Lagoon; Decremento di produzione e cambio nella vegetazione macroalgale nella laguna di Venezia  

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Aim of this work in supplying information on the Ulva rigida C. Ag. distribution and production changes recently monitored in the central part of the lagoon of Venice. This species from 1970s replaced the meadows of rhizophytes which in the past populated the lagoon and nowadays is declining. Estimations of the amounts of nutrients which were involved in the growth of this species before and after its decline are supplied. On the basis of data collected in field during the period 1989-1991, some hypotheses on the main causes promoting these progressive changes are presented.

Sfriso, A. [Venice Univ. (Italy). Dip. di Scienze Ambientali

1996-05-01

233

Metal accumulation and oxidative stress responses in Ulva spp. in the presence of nocturnal pulses of metals from sediment: a field transplantation experiment under eutrophic conditions.  

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In aquatic systems under eutrophic conditions, remobilization of metals from sediment to the overlying water may occur. Consequently, adaptive responses of local organisms could result from the accumulation of metals intermittently released from the sediment. In summer 2007, a field transplantation experiment was performed in the Óbidos lagoon (Portugal) with Ulva spp. comprising three short-term exposures (between 15:30-23:30; 23:30-07:30; 07:30-15:30) during a 24-h period. In each period, Ulva spp. was collected at a reference site located in the lower lagoon (LL) and transplanted to a eutrophic site located at the Barrosa branch (BB), characterized by moderate metal contamination. For comparison purposes, macroalgae samples were simultaneously exposed at LL under the same conditions. Both sites were surveyed in short-time scales (2-4 h) for the analysis of the variability of physical-chemical parameters in the water and metal levels in suspended particulate matter. The ratios to Al of particulate Mn, Fe, Cu and Pb increased during the period of lower water oxygenation at the eutrophic site, reaching 751 × 10??, 0.67, 12 × 10??, 9.9 × 10??, respectively, confirming the release of metals from the sediment to water during the night. At the reference site, dissolved oxygen oscillated around 100%, Mn/Al ratios were considerably lower (81 × 10??-301 × 10??) compared to BB (234 × 10??-790 × 10??), and no increases of metal/Al ratios were found during the night. In general, algae uptake of Mn, Cu, Fe, Pb and Cd was significantly higher at the eutrophic site compared to the reference site. The results confirmed the potential of Ulva spp. as bioindicator of metal contamination and its capability to respond within short periods. An induction of SOD, an inhibition of CAT and the increase of LPO were recorded in Ulva spp. exposed at BB (between 23:30 and 7:30) probably as a response to the higher incorporation of Mn, Fe and Pb in combination with the lack of dissolved oxygen in the water. Current findings emphasize the importance of assessing, in eutrophic systems, the relationship between the variability of chemical conditions and its repercussions on autochthonous organisms over day-night cycles. PMID:24373387

Pereira, Patrícia; de Pablo, Hilda; Guilherme, Sofia; Carvalho, Susana; Santos, Maria Ana; Vale, Carlos; Pacheco, Mário

2014-03-01

234

Copper-induced intracellular calcium release requires extracellular calcium entry and activation of L-type voltage-dependent calcium channels in Ulva compressa  

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The marine alga Ulva compressa exposed to 10 µM copper showed a triphasic increase of intracellular calcium with maximal levels at 2, 3 and 12 h involving the activation of ryanodine-, Ins(1,4,5)P3- and NAADP-sensitive calcium channels. In order to analyze the requirement of extracellular calcium entry for intracellular calcium release as well as the activation of voltage-dependent calcium channels (VDCC) and phospholipase C, U. compressa was treated with EGTA, a non-permeable calcium chelat...

Gonza?lez, Alberto; Los A?ngeles Cabrera, Mari?a; Mellado, Macarena; Cabello, Susana; Ma?rquez, Sebastia?n; Morales, Bernardo; Moenne, Alejandra

2012-01-01

235

Combined effects of light intensity and NH{4/+}-enrichment on growth, pigmentation, and photosynthetic performance of Ulva prolifera (Chlorophyta)  

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The aim of this study was to investigate the effects of light intensity and enhanced nitrogen supply on the growth and photosynthesis of the green-tide macroalga, Ulva prolifera. Thalli of U. prolifera were grown in natural or NH{4/+}-enriched seawater under two different light intensities for 7 days, and then the growth rate, pigmentation, and photosynthetic performance of the thalli were evaluated. The results show that the relative growth rate (RGR) was markedly higher under the high light level than under the low light level. Enrichment with NH{4/+} enhanced the RGR under high light intensity, but did not affect RGR under low light intensity. In low light conditions, NH{4/+} -enrichment resulted in a marked decrease in the maximal photosynthetic rate ( P m) and the maximum carbon fixation rate ( V max), but it did not affect the half saturation constant for carbon ( K 0.5) or the ratio of V max to K 0.5, which reflects the carbon acquisition efficiency. In high light conditions, P m, K 0.5, and the dark respiration rate ( R d) increased under NH{4/+} enrichment, but V max and the V max / K 0.5 ratio decreased. Regardless of the light intensity, NH{4/+}-enrichment did not affect the apparent photosynthetic efficiency ( ?), which reflects the ability of the alga to use light energy at low light levels. Under both low and high light intensities, the chlorophyll a (Chl a), chlorophyll b (Chl b), and carotenoids (Car) contents in thalli were higher in NH{4/+}-enriched than in natural seawater, except that there was a decrease in the Chl b content of thalli in NH{4/+}-enriched seawater under low light intensity. Therefore, NH{4/+} enrichment improved the growth and photosynthetic performance of U. prolifera under high light intensity, but not under low light intensity. We discuss the possible mechanisms underlying these physiological responses.

Xu, Zhiguang; Wu, Haiyi; Zhan, Dongmei; Sun, Fuxin; Sun, Jianzhang; Wang, Guangce

2014-09-01

236

Nitrate and phosphate regimes induced lipidomic and biochemical changes in the intertidal macroalga Ulva lactuca (Ulvophyceae, Chlorophyta).  

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This study was carried out in order to understand the lipid and biochemical alterations resulting from different nutritional regimes of nitrate and phosphate in Ulva lactuca. The algal thalli cultured in artificial seawater (ASW) showed higher levels of carbohydrates and non-polar lipids and increased phosphatase activities, accompanied by degradation of polar lipids, proteins and pigments. Further, higher levels of lipid hydroperoxides indicated reative oxygen species (ROS)-mediated non-enzymatic lipid peroxidation due to nutritional limitation-induced oxidative stress. Those thalli cultured in ASW supplemented with nitrate showed responses corresponding to nitrate addition, such as an increase in pigments, monogalactosyldiacylglycerols, polyunsaturated fatty acids and nitrate reductase. In addition, these thalli showed partial induction of phosphatases, low phospholipids, and high sulfolipid and 1,2-diacylglyceryl-3-O-4'-(N,N,N-trimethyl)-homoserine (DGTS) due to phosphate limitation. Similarly, algal thalli cultured in ASW supplemented with phosphate showed down-regulation of phosphatases, an increase in phospholipids due to availability of phosphate as well as a decrease in nitrate reductase, pigment, monogalactosyldiacylglycerols and polyunsaturated fatty acids due to nitrate limitation. On the other hand, algal thalli cultured in ASW supplemented with both nitrate and phosphate showed recovery of lost pigments and proteins, a high monogalactosyldiacylglycerol/digalactosyldiacylglycerol ratio, high unsaturation and high oxylipin levels (both C18 and C20). Further, the accumulation of indole-3-acetic acid in nutrient-limited thalli and of kinetin and kinetin riboside in nutrient-supplemented thalli indicated their antagonistic roles under nutrient stress. Thus, U. lactuca copes with nitrate and phosphate nutritional stress by altering the metabolic pathways involved in lipid biosynthesis including a shift in lipid classes, fatty acids, oxylipins and indole-3-acetic acid/kinetin cross-talk. PMID:24192295

Kumari, Puja; Kumar, Manoj; Reddy, C R K; Jha, Bhavanath

2014-01-01

237

NMR spectroscopic characterisation of oligosaccharides from two Ulva rigida ulvan samples (Ulvales, Chlorophyta) degraded by a lyase  

International Nuclear Information System (INIS)

The chemical structure and the sequence of repeating units in ulvans of similar compositions from two different Ulva rigida samples collected in the Canary Islands and in Brittany were studied after ulvan-lyase degradation and NMR spectroscopic analysis of the reaction products. Both ulvans were composed of ulvanobiuronic acid 3-sulfate type A[-4)-?-d-GlcA-(1-4)-?-l-Rha 3-sulfate-(1-] (symbolised as A3s) and contained disaccharides composed of [-4)-?-d-Xyl-(1-4)-?-l-Rha 3-sulfate-(1-] and [-4)-?-d-Xyl 2-sulfate-(1-4)-?-l-Rha 3-sulfate], respectively referred to as ulvanobiose 3-sulfate (U3s) and ulvanobiose 2minutes or feet,3-disulfate (U2minutesorfeets,3s). In the Canary Islands sample, these U3s and U2minutesorfeets,3s occurred dispersed among A3s sequences and as short blocks of two or three units. In contrast, in the Brittany samples, these units were dispersed among A3s structures and next to A3s units branched at O-2 of ?-l-Rha 3-sulfate by a terminal ?-d-GlcA and symbolised as A2g,3s. However, more complex structures are likely to occur in the enzyme resistant fraction remaining from this ulvan. An average structure sequence of these two ulvans was proposed. The transposition of the 13C NMR data of the new identified structures to the parent polysaccharides was not possible, probably due to the differesible, probably due to the different sequence distributions affecting the carbons chemical shifts. (Copyright (c) 1998 Elsevier Science B.V., Amsterdam. All rights reserved.)

238

NMR spectroscopic characterisation of oligosaccharides from two Ulva rigida ulvan samples (Ulvales, Chlorophyta) degraded by a lyase  

Energy Technology Data Exchange (ETDEWEB)

The chemical structure and the sequence of repeating units in ulvans of similar compositions from two different Ulva rigida samples collected in the Canary Islands and in Brittany were studied after ulvan-lyase degradation and NMR spectroscopic analysis of the reaction products. Both ulvans were composed of ulvanobiuronic acid 3-sulfate type A[-4)-{beta}-d-GlcA-(1-4)-{alpha}-l-Rha 3-sulfate-(1-] (symbolised as A{sub 3s}) and contained disaccharides composed of [-4)-{beta}-d-Xyl-(1-4)-{alpha}-l-Rha 3-sulfate-(1-] and [-4)-{beta}-d-Xyl 2-sulfate-(1-4)-{alpha}-l-Rha 3-sulfate], respectively referred to as ulvanobiose 3-sulfate (U{sub 3s}) and ulvanobiose 2minutes or feet,3-disulfate (U{sub 2minutes or feets,3s}). In the Canary Islands sample, these U{sub 3s} and U{sub 2minutes or feets,3s} occurred dispersed among A{sub 3s} sequences and as short blocks of two or three units. In contrast, in the Brittany samples, these units were dispersed among A{sub 3s} structures and next to A{sub 3s} units branched at O-2 of {alpha}-l-Rha 3-sulfate by a terminal {beta}-d-GlcA and symbolised as A{sub 2g,3s}. However, more complex structures are likely to occur in the enzyme resistant fraction remaining from this ulvan. An average structure sequence of these two ulvans was proposed. The transposition of the {sup 13}C NMR data of the new identified structures to the parent polysaccharides was not possible, probably due to the different sequence distributions affecting the carbons chemical shifts. (Copyright (c) 1998 Elsevier Science B.V., Amsterdam. All rights reserved.)

Lahaye, M. [Institut National de la Recherche Agronomique, Unite de Recherche sur les Polysaccharides, leurs Organisations et Interactions, BP 71627, F-44316- Nantes (France)

1998-12-21

239

Preliminary study on the responses of three marine algae, Ulva pertusa (Chlorophyta), Gelidium amansii (Rhodophyta) and Sargassum enerve (Phaeophyta), to nitrogen source and its availability  

Science.gov (United States)

An experiment was designed to select economically valuable macroalga species with high nutrient uptake rates. Such species cultured on a large scale could be a potential solution to eutrophication. Three macroalgae species, Ulva pertusa (Chlorophyta), Gelidium amansii (Rhodophyta) and Sargassum enerve (Phaeophyta), were chosen for the experiment because of their economic values and availability. Control and four nitrogen concentrations were achieved by adding NH{4/+} and NO{3/-}. The results indicate that the fresh weights of all species increase faster than that of control after 5 d culture. The fresh weight of Ulva pertusa increases fastest among the 3 species. However, different species show different responses to nitrogen source and its availability. They also show the advantage of using NH{4/+} than using NO{3/-}. U. pertusa grows best and shows higher capability of removing nitrogen at 200µmolL-1, but it has lower economical value. G. amansii has higher economical value but lower capability of removing nitrogen at 200 µmolL-1. The capability of nitrogen assimilation of S. enerve is higher than that of G. amansii at 200µmolL -1, but the former’s increase of fresh weight is lower than those of other two species. Then present preliminary study demonstrates that it is possible to use macroalgae as biofilters and further development of this approach could provide biologically valuable information on the source, fate, and transport of N in marine ecosystems. Caution is needed should we extrapolate these findings to natural environments.

Liu, Dongyan; Amy, Pickering; Sun, Jun

2004-04-01

240

Ulva and Enteromorpha (Ulvaceae, Chlorophyta) from two sides of the Yellow Sea: analysis of nuclear rDNA ITS and plastid rbcL sequence data  

Science.gov (United States)

Ulvacean green seaweeds are common worldwide; they formed massive green tides in the Yellow Sea in recent years, which caused marine ecological problems as well as a social issue. We investigated two major genera of the Ulvaceae, Ulva and Enteromorpha, and collected the plastid rbcL and nuclear ITS sequences of specimens of the genera in two sides of the Yellow Sea and analyzed them. Phylogenetic trees of rbcL data show the occurrence of five species of Enteromorpha ( E. compressa, E. flexuosa, E. intestinalis, E. linza and E. prolifera) and three species of Ulva ( U. pertusa, U. rigida and U. ohnoi). However, we found U. ohnoi, which is known as a subtropical to tropical species, at two sites on Jeju Island, Korea. Four ribotypes in partial sequences of 5.8S rDNA and ITS2 from E. compressa were also found. Ribotype network analysis revealed that the common ribotype, occurring in China, Korea and Europe, is connected with ribotypes from Europe and China/Japan. Although samples of the same species were collected from both sides of the Yellow Sea, intraspecific genetic polymorphism of each species was low among samples collected worldwide.

Wang, Jinfeng; Li, Nan; Jiang, Peng; Boo, Sung Min; Lee, Wook Jae; Cui, Yulin; Lin, Hanzhi; Zhao, Jin; Liu, Zhengyi; Qin, Song

2010-07-01

 
 
 
 
241

Phylogenetic analyses of four species of Ulva and Monostroma grevillei using ITS, rbc L and 18S rDNA sequence data  

Science.gov (United States)

Chlorophyta species are common in the southern and northern coastal areas of China. In recent years, frequent green tide incidents in Chinese coastal waters have raised concerns and attracted the attention of scientists. In this paper, we sequenced the 18S rDNA genes, the internal transcribed spacer (ITS) regions and the rbc L genes in seven organisms and obtained 536-566 bp long ITS sequences, 1 377-1 407 bp long rbc L sequences and 1 718-1 761 bp long partial 18S rDNA sequences. The GC base pair content was highest in the ITS regions and lowest in the rbc L genes. The sequencing results showed that the three Ulva prolifera (or U. pertusa) gene sequences from Qingdao and Nan'ao Island were identical. The ITS, 18S rDNA and rbc L genes in U. prolifera and U. pertusa from different sea areas in China were unchanged by geographic distance. U. flexuosa had the least evolutionary distance from U. californica in both the ITS regions (0.009) and the 18S rDNA (0.002). These data verified that Ulva and Enteromorpha are not separate genera.

Lin, Zhongheng; Shen, Songdong; Chen, Weizhou; Li, Huihui

2013-01-01

242

Ecologia populacional dos Amphipoda (Crustacea dos fitais de Caiobá, Matinhos, Paraná, Brasil Population ecolocy of Amphipoda (Crustacea from the phytals of Caiobá, Matinhos, Paraná, Brazil  

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Full Text Available Spalial and temporal density distributions of Amphipoda from the phytals of Caiobá are described. Air temperature oscillated from 16ºC (August and May to 23ºC (March, surface water temperature from 17ºC (August to 25ºC (March and the salinity from 29.3‰ (May to 32.8‰ (August. Two samples of 25cm² (for algae less than 5cm long, 100 cm² (for algae between 5-10cm long and whole plants (for algae more than 10cm long were removed with a spatula from the rocky surface at Caiobá Beach, in August/86, November/86, March/87 and May/87. After sorting, the algal substrata were weighted, their adsorption coefficient calculated and the sediment retained among the thallii weighted. The average distance between the branching was measured for all branched algae. The densities were calculated in relation to the weight of the algal substrate in grams. Eight phytals were considered: Ulva fasciata Delile, Padina gymnospora (Kútzing Vickers, Sargassum cymosum Garth, Porphyra atropurpurea (Olivi De Toni, Gelidium sp., Gymnogongrus griffithsiae (Turner Martius, Pterocladia capillacea (Gmelin Bornet &Thurel and Pterosiphonia pennata (Roth Falkenberg, over which nine Amphipoda species live: Ampithoe ramondi Audouin, 1816, Cymadusa filosa Savigny, 1852, Elasmopus pectenicrus Bate, 1857, Hyale media Dana, 1857, Hyale sp.l, Jassa falcata Montagu, 1895 and Sunampithoe pelagica H. Milne-Edwards, 1830 (Gammaridea. Caprella danilevskii Czerniavski, 1861 and Caprella penantis Leach, 1814 (Caprellidea. Amphipoda densities ranged from 0.27 ind.g-1 to 45.68. ind.g-1. The broad-thallii algae Porphyra, Ulva and Padina harbored lower densities of Amphipoda, whereas those finely branched Pterocladia, Pterosiphonia and Gymnogongrus, the highest values and the less branched Sargassum and Gelidium, intermediate values. The high densities found in the finely branched algae had as main contribution the juvenile recruiting of most Amphipoda. The tide level might have influenced the temporal distribution of the Amphipoda density, due to the distinct time of air exposition in eaeh collection data. Most Amphipoda did not show specific algal substratum colonization: only Sunampithoe pelagica occurred solely in Sargassum. Four species occurred in different branched algae: J. falcata. S. pelagica. C. danilevskii and C. penantis. H. media had Sargassum, Pterocladia, Pterosiphonia and Gymnogongrus as the best algal substrata, whereas Caprellidea, the Pterocladia and Pterosiphonia phytals. High sediment weight in Padina was the main reason for high densities of Hyale sp.l in this phytal. The occurrence of males, females (including ovigerous ones and juveniles of most Amphipoda species found in the present study indicates a complete life cycle whithin these phytals and corroborates with the assumption of the complexity of this marine coastal ecosystem.

Janete Dubiaski-Silva

1995-01-01

243

Marine Bacteria from Danish Coastal Waters Show Antifouling Activity against the Marine Fouling Bacterium Pseudoalteromonas sp. Strain S91 and Zoospores of the Green Alga Ulva australis Independent of Bacteriocidal Activity  

DEFF Research Database (Denmark)

The aims of this study were to determine if marine bacteria from Danish coastal waters produce antifouling compounds and if antifouling bacteria could be ascribed to specific niches or seasons. We further assess if antibacterial effect is a good proxy for antifouling activity. We isolated 110 bacteria with anti-Vibrio activity from different sample types and locations during a 1-year sampling from Danish coastal waters. The strains were identified as Pseudoalteromonas, Phaeobacter, and Vibrionaceae based on phenotypic tests and partial 16S rRNA gene sequence similarity. The numbers of bioactive bacteria were significantly higher in warmer than in colder months. While some species were isolated at all sampling locations, others were niche specific. We repeatedly isolated Phaeobacter gallaeciensis at surfaces from one site and Pseudoalteromonas tunicata at two others. Twenty-two strains, representing the major taxonomic groups, different seasons, and isolation strategies, were tested for antiadhesive effect against the marine biofilm-forming bacterium Pseudoalteromonas sp. strain S91 and zoospores of the green alga Ulva australis. The antiadhesive effects were assessed by quantifying the number of strain S91 or Ulva spores attaching to a preformed biofilm of each of the 22 strains. The strongest antifouling activity was found in Pseudoalteromonas strains. Biofilms of Pseudoalteromonas piscicida, Pseudoalteromonas tunicata, and Pseudoalteromonas ulvae prevented Pseudoalteromonas S91 from attaching to steel surfaces. P. piscicida killed S91 bacteria in the suspension cultures, whereas P. tunicata and P. ulvae did not; however, they did prevent adhesion by nonbactericidal mechanism(s). Seven Pseudoalteromonas species, including P. piscicida and P. tunicata, reduced the number of settling Ulva zoospores to less than 10% of the number settling on control surfaces. The antifouling alpP gene was detected only in P. tunicata strains (with purple and yellow pigmentation), so other compounds/mechanisms must be present in theother Pseudoalteromonas strains with antifouling activity.

Bernbom, Nete; Gram, Lone

2011-01-01

244

A novel marine algal toxicity bioassay based on sporulation inhibition in the green macroalga Ulva pertusa (Chlorophyta).  

Science.gov (United States)

A 5-day aquatic toxicity test based on sporulation inhibition of the green macroalga Ulva pertusa Kjellman has been developed. Optimal test conditions determined for photon irradiance, salinity and temperature were 60-200 micromol photons m(-2)s(-1), 25-35 per thousand and 15-20 degrees C, respectively. Tests were conducted by exposing U. pertusa thallus disks to a reference toxicant (sodium dodecyl sulfate; SDS), metals (Cd2+, Cu2+, Zn2+, Pb2+) and elutriates of sludge collected from nine different locations. The EC(50) values for SDS was 5.35 mg L(-1). When four heavy metals were assayed, the NOECs were highest for lead (0.625 mg L(-1)) and lowest for copper (0.031 mg L(-1)). The EC50 values showed the following toxicity rankings: Cu(2+) (0.061 mg L(-1))>Cd2+ (0.326 mg L(-1))>Zn2+ (0.738 mg L(-1))>Pb2+ (0.877 mg L(-1)). The bioassay indicated also that the sporulation endpoint could be a sensitive indicator of toxicity effects of elutriates of sludge as reflected from the NOEC values equal to or lower than the lowest concentration employed (6.25%). Sporulation was significantly inhibitied in all elutriates with the greatest and least effects observed in elutriates of sludge from industrial waste (EC50 6.78%) and filtration bed (EC50 15.0%), respectively. The results of the Spearman rank correlation analysis for EC50 data versus the concentrations of toxicants in the sludge presented a significant correlation between toxicity and four heavy metals (Cd2+, Cu2+, Pb2+, Zn2+). Introduction of the concept of toxicity unit (TU) showed that these metals were the main cause of toxicity in elutriates of at least four out of nine sludge samples. Members of the order Ulvales show a wide geographic distribution and have similar reproductive characteristics, thus making it possible to apply the present test method to other algae of this taxa, elsewhere. This novel method will be a useful tool for assessing the aquatic toxicity of a wide range of toxicants, once the respective sensitivities are demonstrated. PMID:16157398

Han, Taejun; Choi, Gye-Woon

2005-11-10

245

A novel marine algal toxicity bioassay based on sporulation inhibition in the green macroalga Ulva pertusa (Chlorophyta)  

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A 5-day aquatic toxicity test based on sporulation inhibition of the green macroalga Ulva pertusa Kjellman has been developed. Optimal test conditions determined for photon irradiance, salinity and temperature were 60-200 {mu}mol photons m{sup -2} s{sup -1}, 25-35%o and 15-20 deg C, respectively. Tests were conducted by exposing U. pertusa thallus disks to a reference toxicant (sodium dodecyl sulfate; SDS), metals (Cd{sup 2+}, Cu{sup 2+}, Zn{sup 2+}, Pb{sup 2+}) and elutriates of sludge collected from nine different locations. The EC{sub 50} values for SDS was 5.35 mg L{sup -1}. When four heavy metals were assayed, the NOECs were highest for lead (0.625 mg L{sup -1}) and lowest for copper (0.031 mg L{sup -1}). The EC{sub 50} values showed the following toxicity rankings: Cu{sup 2+} (0.061 mg L{sup -1}) > Cd{sup 2+} (0.326 mg L{sup -1}) > Zn{sup 2+} (0.738 mg L{sup -1}) > Pb{sup 2+} (0.877 mg L{sup -1}). The bioassay indicated also that the sporulation endpoint could be a sensitive indicator of toxicity effects of elutriates of sludge as reflected from the NOEC values equal to or lower than the lowest concentration employed (6.25%). Sporulation was significantly inhibitied in all elutriates with the greatest and least effects observed in elutriates of sludge from industrial waste (EC{sub 50} 6.78%) and filtration bed (EC{sub 50} 15.0%), respectively. The results of the Spearman rank correlation analysis for EC{sub 50} data versus the concentrations of toxicants in the sludge presented a significant correlation between toxicity and four heavy metals (Cd{sup 2+}, Cu{sup 2+}, Pb{sup 2+}, Zn{sup 2+}). Introduction of the concept of toxicity unit (TU) showed that these metals were the main cause of toxicity in elutriates of at least four out of nine sludge samples. Members of the order Ulvales show a wide geographic distribution and have similar reproductive characteristics, thus making it possible to apply the present test method to other algae of this taxa, elsewhere. This novel method will be a useful tool for assessing the aquatic toxicity of a wide range of toxicants, once the respective sensitivities are demonstrated.

Han, Taejun [Division of Biology and Chemistry, University of Incheon, Incheon 402-749 (Korea, Republic of)]. E-mail: hanalgae@incheon.ac.kr; Choi, Gye-Woon [Department of Civil and Environmental System Engineering, University of Incheon, Incheon 402-749 (Korea, Republic of)

2005-11-10

246

De novo sequencing and analysis of the Ulva linza transcriptome to discover putative mechanisms associated with its successful colonization of coastal ecosystems  

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Full Text Available Abstract Background The green algal genus Ulva Linnaeus (Ulvaceae, Ulvales, Chlorophyta is well known for its wide distribution in marine, freshwater, and brackish environments throughout the world. The Ulva species are also highly tolerant of variations in salinity, temperature, and irradiance and are the main cause of green tides, which can have deleterious ecological effects. However, limited genomic information is currently available in this non-model and ecologically important species. Ulva linza is a species that inhabits bedrock in the mid to low intertidal zone, and it is a major contributor to biofouling. Here, we presented the global characterization of the U. linza transcriptome using the Roche GS FLX Titanium platform, with the aim of uncovering the genomic mechanisms underlying rapid and successful colonization of the coastal ecosystems. Results De novo assembly of 382,884 reads generated 13,426 contigs with an average length of 1,000 bases. Contiguous sequences were further assembled into 10,784 isotigs with an average length of 1,515 bases. A total of 304,101 reads were nominally identified by BLAST; 4,368 isotigs were functionally annotated with 13,550 GO terms, and 2,404 isotigs having enzyme commission (EC numbers were assigned to 262 KEGG pathways. When compared with four other full sequenced green algae, 3,457 unique isotigs were found in U. linza and 18 conserved in land plants. In addition, a specific photoprotective mechanism based on both LhcSR and PsbS proteins and a C4-like carbon-concentrating mechanism were found, which may help U. linza survive stress conditions. At least 19 transporters for essential inorganic nutrients (i.e., nitrogen, phosphorus, and sulphur were responsible for its ability to take up inorganic nutrients, and at least 25 eukaryotic cytochrome P450s, which is a higher number than that found in other algae, may be related to their strong allelopathy. Multi-origination of the stress related proteins, such as glutamate dehydrogenase, superoxide dismutases, ascorbate peroxidase, catalase and heat-shock proteins, may also contribute to colonization of U. linza under stress conditions. Conclusions The transcriptome of U. linza uncovers some potential genomic mechanisms that might explain its ability to rapidly and successfully colonize coastal ecosystems, including the land-specific genes; special photoprotective mechanism based on both LhcSR and PsbS; development of C4-like carbon-concentrating mechanisms; muti-origin transporters for essential inorganic nutrients; multiple and complex P450s; and glutamate dehydrogenase, superoxide dismutases, ascorbate peroxidase, catalase, and heat-shock proteins that are related to stress resistance.

Zhang Xiaowen

2012-10-01

247

Comparative studies on utilizing nitrogen capacity between two macroalgae Gracilaria tenuistipitata var. liui (rhodophyta) and Ulva pertusa (chlorophyta). II. Feedback controls of intracellular nitrogen pools on nitrogen uptake.  

Science.gov (United States)

The potential feedback by intracellular nitrogen pools on maximum N uptake (NH4+) rate were determined for Gracilaria tenuistipitata var. liui and Ulva pertusa. The results of correlation matrix analyzing showed that the surge uptake of ammonium seemed related to rapid changes in small intracellular pools of inorganic nitrogen or small peptide and amino acids rather than to changes in TN content of the macroalgae. The assimilation rates of nitrogen of U. pertusa and G. tenuistipitata increased slowly during N starvation and were mainly regulated by amino acids and some incorporation of amino acids into macromolecules. From ecological point of view, the fast-growing and uptaking nutrient U. pertusa is more suitable to improve water quality in integrated shrimp culture ponds in which external nutrient supplies are usually high and constant during the culture period, while G. tenuistipitata var. liui is more suitable to be polycultured in a waters with intermittence supply of nutrients. PMID:11590764

Liu, J W; Dong, S L

2001-07-01

248

Spectral definition of the macro-algae Ulva curvata in the back-barrier bays of the Eastern Shore of Virginia, USA  

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We have developed methods to determine the visible (VIS) to near-infrared (NIR) spectral properties of thalli and epiphytes of bloom-forming and green macrophyte Ulva curvata in back-barrier lagoons in Virginia, USA. A 2% increase in NIR thalli reflectance from winter to summer matched the drop in summer NIR transmittance. In contrast, summer and winter VIS reflectance were nearly identical while winter transmittance was 10-20% higher. NIR absorption remained at 5% but VIS absorption increased by 10-20% from winter to summer. Replicate consistency substantiated the high transmittance difference indicating thallus composition changed from summer to winter. Epiphytes increased thallus reflectance and decreased transmittance and exhibited broadband VIS and NIR absorptions in summer and selective peaks in winter. A simulation coupling water extinction with thallus reflectance and transmittance found seven submerged thalli maximized the surface reflectance enhancement.

Ramsey, Elijah W., III; Rangoonwala, Amina; Solgaard, Mads; Schwarzchild, Arthur

2012-01-01

249

Cultivo y valoración de Hydropuntia cornea (Rhodophyta) y Ulva rigida (Chlorophyta) para la producción de biodiesel: dinámica de la disponibilidad de amonio y de las condiciones de irradiación sobre la calidad de la biomasa  

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Las macroalgas Hydropuntia cornea (Rhodophyta) y Ulva rigida (Chlorophyta) fueron cultivadas en tanques de 90 L de manera intensiva; a escala de planta piloto en un sistema de biofiltración de aguas ricas en nutrientes (N-amonio y P-fosfato) y bajo diferentes condiciones de irradiación: (1) Exterior (outdoor) con radiación natural y, (2) bajo condiciones de invernadero (80% PAR + 10% UVA + 0% UVB), en las instalaciones de cultivo del Centro de Biotecnología Marina (Taliarte...

Gonza?lez-gonza?lez, Rayco

2010-01-01

250

Dicty_cDB: Contig-U13894-1 [Dicty_cDB  

Full Text Available Contig-U13894-1 no gap 1550 2 2081463 2079913 MINUS 30 31 U13894 1 0 15 0 9 1 1 0 1 1 1 0 0 0 Sh ... ypoth... 220 4e-69 DQ286546_1( DQ286546 |pid:none) Ulva ... fasciata glutathione reductas... 169 9e-69 AP00725 ...

251

Synseed production for storage and conservation of ochradenus baccatus delile  

International Nuclear Information System (INIS)

Ochradenus baccatus is a medicinal plant of high value, spread over sandy and stony places of Kingdom of Saudi Arabia and most of the desert regions of Egypt. This species contains several flavanoids and specific constituents which are important as these have already been effectively used in lowering cholesterol in the blood of rats and high inhibition potential of the malarial parasite (Plasmodium falciparum). Synseeds were produced from stem segments and apical bud of O. baccatus growing In vitro. Two sets of synseeds were produced, one non-dried and the other dried under running laminar air-flow for 30 min. Regeneration and regrowth were evaluated for 16 weeks storage under various temperatures (4, 8 and 12degree C). The maximum frequency of conversion into plantlets was achieved on the MS medium containing 1.0 micro M BA in encapsulated nodal segments stored at 4 degree C. Rooting in these shoots was induced by the pulse treatment of 100 micro M IBA for 10 days, and the rooted shoots were transferred on the MS medium devoid of any PGR. Fair percent rooting occurred after one week of transfer on the MS medium. Plantlets were successfully established. No phenotypic variations were observed between the synseed originated plants with mother plant. Genetic stability of synseed grown plants and mother plant was evaluated by inter-simple sequence repeat (ISSR) marker. The mother plant as well as regenerated plants from synseed resulted in a monomorphic banding pattern developed from ISSR markers confirming genetic stability among the clones. This protocol will help multiply and conserve the plant as well as for short-term storage of germplasm for commercial use and exchange. (author)

252

Activity of commercial enzymes on settlement and adhesion of cypris larvae of the barnacle Balanus amphitrite, spores of the green alga Ulva linza, and the diatom Navicula perminuta.  

Science.gov (United States)

Fouling species produce adhesive polymers during the settlement, adhesion and colonization of new surfaces in the marine environment. The present paper tests the hypothesis that enzymes of the appropriate specificity may prevent biofouling by hydrolysing these adhesive polymers. Seventeen commercially available enzyme preparations designed originally for bulk use in a range of end-use applications were tested for their effects on the settlement and/or adhesion of three major fouling species, viz. the green alga Ulva linza, the diatom Navicula perminuta and the barnacle Balanus amphitrite. The serine-proteases were found to have the broadest antifouling potential reducing the adhesion strength of spores and sporelings of U. linza, cells of N. perminuta and inhibiting settlement of cypris larvae of B. amphitrite. Mode-of-action studies on the serine-protease, Alcalase, indicated that this enzyme reduced adhesion of U. linza in a concentration-dependent manner, that spores of the species could recover their adhesive strength if the enzyme was removed and that the adhesive of U. linza and juvenile cement of B. amphitrite became progressively less sensitive to hydrolysis as they cured. PMID:15804714

Pettitt, M E; Henry, S L; Callow, M E; Callow, J A; Clare, A S

2004-12-01

253

Allelopathic effects of Ulva pertusa, Corallina pilulifera and Sargassum thunbergii on the growth of the dinoflagellates Heterosigma akashiwo and Alexandrium tamarense  

Science.gov (United States)

The allelopathic effects of fresh tissue, dry powder and aqueous extracts of three macroalgae, Ulva pertusa, Corallina pilulifera and Sargassum thunbergii, on the growth of the dinoflagellates Heterosigma akashiwo and Alexandrium tamarense were evaluated using coexistence culture systems in which concentrations of the three macroalga were varied. The results of the coexistence assay showed that the growth of the two microalgae was strongly inhibited by using fresh tissue, dry powder and aqueous extracts of the three macroalga; the allelochemicals were lethal to H. akashiwo at relatively higher concentrations of the three macroalga. The macroalgae showing the most allelopathic effect on H. akashiwo and A. tamarense using fresh tissue were U. pertusa and S. thunbergii, using dry powder were S. thunbergii and U. pertusa, and using aqueous extracts were U. pertusa and C. pilulifera. We also examined the potential allelopathic effect on the two microalgae of culture filtrate of the three macroalga; culture medium filtrate initially exhibited no inhibitory effects when first added but inhibitory effects became apparent under semi-continuous addition, which suggested that continuous release of small quantities of rapidly degradable allelochemicals from the fresh macroalgal tissue were essential to effectively inhibit the growth of the two microalgae. PMID:19396350

Wang, Renjun; Xiao, Hui; Zhang, Peiyu; Qu, Liang; Cai, Hengjiang

2006-01-01

254

Fouling coverage of a green tide alga, Ulva pertusa on some antifouling test surfaces exposed to Ayagin harbor waters, east coast of South Korea.  

Science.gov (United States)

Toxic antifouling chemicals released into the seawaters leads to marine environmental degradation. In order to identify a nontoxic antifoulant, an assessment of antifouling (AF) efficacy of some AF candidates was made at Ayagin harbor, east coast of South Korea. In this static panel study conducted during October 2000-March 2001, some commercial antifoulants, seaweed and seagrass extracts were screened. On panel surfaces coated with a seaweed extract, Ishige okamurae exhibited effective AF activity. Ulva pertusa was encountered as a 'monospecific' fouler with fairly high fouling coverage on many of the test panel surfaces. In recent years the increased influx of inorganic pollutants in the coastal waters causes exorbitant growth of fouling marine algae found all along the Korean peninsula. Especially, a cosmopolitan ship fouling alga U. pertusa occur with high abundance. It was largely suggested that the proposed international ban on the toxic antifoulant tributyltin (TBT) had significant effect on the 'green tide' phenomenon occurring in different parts of the world. However, it appears that Korean scenario of 'green tide' is a localized. Antifouling efficacy of some AF coatings and fouling coverage of a green tide alga, U. pertusa are discussed. PMID:15303702

Sidharthan, M; Shin, Hyun Woung; Joo, Jin Hyung

2004-01-01

255

Comparative studies on utilizing nitrogen capacity between two macroalgae Gracilaria tenuistipitata var. liui (rhodophyta) and Ulva pertusa (chlorophyta). I. Nitrogen storage under nitrogen enrichment and starvation.  

Science.gov (United States)

This paper deals with the N storage of Gracilaria tenuistipitata var. liui and Ulva pertusa under ammonium enrichment and starvation. After 10 days of ammonium enrichment, ammonium NH4+, free amino acid (FAA), protein (pro), chlorophyll (Chl), phycoerythrin (PE) and total dissolved nitrogen (TDN) of the two macroalgae increased significantly. Total nitrogen (TN) increased significantly from 3.65% to 5.78% dry weight of G. tenuistipitata var. liui and 2.82% to 5.07% dw of U. pertusa, respectively. Protein and FAA were the most important N storage pools in the macroalgae. During N-starvation period, individual N pools of the two species were depleted at exponential rates. Each N pool in U. pertusa decreased more rapidly than in G. tenuistipitata, var. liui and the latter was able to sustain fast growth for more time (> 20 days) than U. pertusa. N demands for supporting growth were different between the two species, U. pertusa grew more rapidly and had higher N demands than G. tenuistipitata var. liui did. PMID:11590763

Liu, J W; Dong, S L

2001-07-01

256

Allelopathic effects of Ulva pertusa, Corallina pilulifera and Sargassum thunbergii on the growth of the dinoflagellates Heterosigma akashiwo and Alexandrium tamarense.  

Science.gov (United States)

The allelopathic effects of fresh tissue, dry powder and aqueous extracts of three macroalgae, Ulva pertusa, Corallina pilulifera and Sargassum thunbergii, on the growth of the dinoflagellates Heterosigma akashiwo and Alexandrium tamarense were evaluated using coexistence culture systems in which concentrations of the three macroalga were varied. The results of the coexistence assay showed that the growth of the two microalgae was strongly inhibited by using fresh tissue, dry powder and aqueous extracts of the three macroalga; the allelochemicals were lethal to H. akashiwo at relatively higher concentrations of the three macroalga. The macroalgae showing the most allelopathic effect on H. akashiwo and A. tamarense using fresh tissue were U. pertusa and S. thunbergii, using dry powder were S. thunbergii and U. pertusa, and using aqueous extracts were U. pertusa and C. pilulifera. We also examined the potential allelopathic effect on the two microalgae of culture filtrate of the three macroalga; culture medium filtrate initially exhibited no inhibitory effects when first added but inhibitory effects became apparent under semi-continuous addition, which suggested that continuous release of small quantities of rapidly degradable allelochemicals from the fresh macroalgal tissue were essential to effectively inhibit the growth of the two microalgae. PMID:19396350

Wang, Renjun; Xiao, Hui; Zhang, Peiyu; Qu, Liang; Cai, Hengjiang; Tang, Xuexi

2007-04-01

257

Isolation and characterization of a cDNA encoding a heat shock protein 70 from a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta).  

Science.gov (United States)

Synthesis and accumulation of molecular chaperones are universal responses found in all cellular organisms when exposed to a variety of unfavorable conditions. Heat shock protein 70 (Hsp70), which is one of the major classes of molecular chaperones, plays a particularly important role in cellular stress responses, and the Hsp70 system is the most intensely studied in higher plants and algae. Therefore, we isolated and characterized a cDNA clone encoding Hsp70 from a sterile strain of Ulva pertusa (Ulvales, Chlorophyta). The sterile U. pertusa Hsp70 (UpHsp70) cDNA consisted of 2,272 nucleotides and had an open reading frame encoding a polypeptide of 663 amino acid (AA) residues with a molecular mass of 71.7 kDa. Amino acid alignment and phylogenetic analysis of Hsp70s from other organisms showed that UpHsp70 was more similar to cytoplasmic Hsp70s from green algae and higher plants (> or =75%) than to those from other algae and microorganisms. Southern blot analysis indicated that the sterile U. pertusa genome had at least four cytoplasmic Hsp70-encoding genes. UpHsp70 mRNA levels were significantly affected by diurnal changes, rapidly increased by high-temperature stress, and gradually increased by exposure to copper, cadmium, and lead. These results suggest that UpHsp70 plays particularly important roles in adaptation to high-temperature conditions and diurnal changes, and is potentially involved in tolerance to heavy metal toxicity. PMID:20111994

Tominaga, Hiroshi; Coury, Daniel Adam; Amano, Hideomi; Kakinuma, Makoto

2010-03-01

258

Preparation of novel alginate based anion exchanger from Ulva japonica and its application for the removal of trace concentrations of fluoride from water.  

Science.gov (United States)

A green seaweed, Ulva japonica, was modified by loading multivalent metal ions such as Zr(IV) and La(III) after CaCl2 cross-linking to produce metal loaded cross-linked seaweed (M-CSW) adsorbents, which were characterized by elemental analysis, functional groups identification, and metal content determination. Maximum sorption potential for fluoride was drastically increased after La(III) and Zr(IV) loading, which were evaluated as 0.58 and 0.95 mmol/g, respectively. Loaded fluoride was quantitatively desorbed by using dilute alkaline solution for its regeneration. Mechanism of fluoride adsorption was inferred in terms of ligand exchange reaction between hydroxyl ion on co-ordination sphere of the loaded metal ions of M-CSW and fluoride ion in aqueous solution. Application of M-CSW for the treatment of actual waste plating solution exhibited successful removal of fluoride to clear the effluent and environmental standards in Japan, suggesting high possibility of its application for the treatment of fluoride rich waste water. PMID:24055965

Paudyal, Hari; Pangeni, Bimala; Inoue, Katsutoshi; Kawakita, Hidetaka; Ohto, Keisuke; Ghimire, Kedar Nath; Alam, Shafiq

2013-11-01

259

?(15)N variation in Ulva lactuca as a proxy for anthropogenic nitrogen inputs in coastal areas of Gulf of Gaeta (Mediterranean Sea).  

Science.gov (United States)

We tested the capacity of Ulva lactuca to mark N sources across large marine areas by measuring variation in its ?(15)N at several sites in the Gulf of Gaeta. Comparisons were made with the macroalga Cystoseira amentacea. Variation of ?(15)N values was assessed also in the coastal waters off the Circeo Natural Park, where U. lactuca and C. amentacea were harvested, as these waters are barely influenced by human activities and were used as reference site. A small fragment from each frond was preserved before deployment in order to characterize the initial isotopic values. After 48 h of submersion, U. lactuca was more responsive than C. amentacea to environmental variation and ?(15)N enrichment in the Gulf of Gaeta was observed. The spatial distribution of ?(15)N enrichment indicated that different macro-areas in the Gulf were affected by N inputs from different origins. Comparison of the ?(15)N values of fragments taken from the same transplanted frond avoided bias arising from natural isotopic variability. PMID:24923814

Orlandi, Lucia; Bentivoglio, Flavia; Carlino, Pasquale; Calizza, Edoardo; Rossi, David; Costantini, Maria Letizia; Rossi, Loreto

2014-07-15

260

Effects of three macroalgae, Ulva linza (Chlorophyta), Corallina pilulifera (Rhodophyta) and Sargassum thunbergii (Phaeophyta) on the growth of the red tide microalga Prorocentrum donghaiense under laboratory conditions  

Science.gov (United States)

Allelopathic effects of several concentrations of fresh tissue and dry powder of three macroalgae, Ulva linza, Corallina pilulifera and Sargassum thunbergii, on the red tide microalga Prorocentrum donghaiense were evaluated in microcosms. Preliminary studies on the algicidal effects of one aqueous and four organic solvent extracts from the macroalgae on the microalga were carried out to confirm the existence of allelochemicals in the tissues of the macroalgae. The effects of macroalgal culture medium filtrate on P. donghaiense were investigated using initial or semi-continuous filtrate addition. Furthermore, the potential effects of the microalga on these three macroalgae were also tested. The results of the microcosm assay showed that the growth of P. donghaiense was strongly inhibited by using fresh tissues and dry powder of the three macroalgae. Both aqueous and methanol extracts of the macroalgae had strong growth inhibitory effects on P. donghaiense, while the other three organic solvent extracts (acetone, ether and chloroform) had no apparent effect on its growth; this suggested that the allelochemicals from these three macroalga had relatively high polarities. The three macroalgal culture medium filtrates exhibited apparent growth inhibitory effect on the microalgae under initial or semi-continuous addition, which suggested that the cells of P. donghaiense are sensitive to the allelochemicals. In contrast, P. donghaiense had no apparent effect on the growth of the macroalgae in coexistence experiment.

Wang, Renjun; Xiao, Hui; Wang, You; Zhou, Wenli; Tang, Xuexi

2007-10-01

 
 
 
 
261

Ti?m n?ng rong Ulva sp. trong l?c sinh h?c và s?n xu?t n?ng l??ng sinh h?c  

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In order to evaluate the effect of seaweeds in bio-filtration for removing nitrogen from marine aquaculture and in bioenergy production, Ulva sp. was used in this study. Experiments were triplicated and run in 3-day incubation at salinities with 30 psu, 10 psu and 5 psu in different initial ammonium nitrogen concentrations from 100 ?M to 10,000 ?M, equivalently to marine aquaculture conditions. The highest concentrations of ammonium removed were about 690 ?mol (12.42 mg) NH4+ at 30 psu, 41...

Dang, Thom Thi; Yasufumi, Mishima; Dang, Kim Dinh

2012-01-01

262

Photosystem I shows a higher tolerance to sorbitol-induced osmotic stress than photosystem II in the intertidal macro-algae Ulva prolifera (Chlorophyta).  

Science.gov (United States)

The photosynthetic performance of the desiccation-tolerant, intertidal macro-algae Ulva prolifera was significantly affected by sorbitol-induced osmotic stress. Our results showed that photosynthetic activity decreased significantly with increases in sorbitol concentration. Although the partial activity of both photosystem I (PS I) and photosystem II (PS II) was able to recover after 30?min of rehydration, the activity of PS II decreased more rapidly than PS I. At 4?M sorbitol concentration, the activity of PS II was almost 0 while that of PS I was still at about one third of normal levels. Following prolonged treatment with 1 and 2?M sorbitol, the activity of PS I and PS II decreased slowly, suggesting that the effects of moderate concentrations of sorbitol on PS I and PS II were gradual. Interestingly, an increase in non-photochemical quenching occurred under these conditions in response to moderate osmotic stress, whereas it declined significantly under severe osmotic stress. These results suggest that photoprotection in U. prolifera could also be induced by moderate osmotic stress. In addition, the oxidation of PS I was significantly affected by osmotic stress. P700(+) in the thalli treated with high concentrations of sorbitol could still be reduced, as PS II was inhibited by 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), but it could not be fully oxidized. This observation may be caused by the higher quantum yield of non-photochemical energy dissipation in PS I due to acceptor-side limitation (Y(NA)) during rehydration in seawater containing DCMU. PMID:24628656

Gao, Shan; Zheng, Zhenbing; Gu, Wenhui; Xie, Xiujun; Huan, Li; Pan, Guanghua; Wang, Guangce

2014-10-01

263

Annually recurrent macroalgal blooms (Ulva prolifera) resulting in the world's largest green-tides caused by expansion of coastal aquaculture in the Yellow Sea off China  

Science.gov (United States)

The largest macroalgal blooms ever recorded occurred in the Yellow Sea of China in 2008 and 2009 and resulted in extensive green tides along the Shandong Province coastline, including at Qingdao. At their peak these Ulva prolifera blooms covered more than 4,000 km2 and affected 40,000 km2. A smaller bloom was recorded in 2007, but not earlier. Since then massive blooms have occurred annually in summer from 2008 to 2012. Using remote sensing methods, we tracked the source of the 2008 and 2009 blooms to an area along the Jiangsu Province coastline near Yancheng, over 200 km south of Qingdao, where there had been rapid expansion of Porphyra aquaculture to as much as 13 km offshore, prior to the appearance of the first bloom in 2007. Porphyra is grown on rafts which can become heavily fouled with U. prolifera which is disposed of into the sea when the Porphyra is harvested. The timing of the blooms occurred post the April harvest period when daily tidal ranges in this region can be in excess of 7 m. This provides the mechanism for transportation of the floating algae offshore and into the warm nutrient rich waters of the Yellow Sea where it grows rapidly forming large patches. As the patches of algae grow and join, they gradually move north, as a result of wind driven surface currents that prevail in the Yellow Sea in summer, ultimately washing ashore on the Shandong Peninsula. We present a range of oceanographic, biological, ecological and genetic data to support the hypothesis that Porphyra aquaculture provides the source biomass for the Yellow Sea green-tides. Improved aquaculture waste disposal methods in the southern area of Jiangsu Province are likely to reduce or prevent the Yellow Sea green tides and present a feasible solution to a recurrent problem.

Keesing, John; Liu, Dongyan

2013-04-01

264

Detecting massive green algae ( Ulva prolifera) blooms in the Yellow Sea and East China Sea using Geostationary Ocean Color Imager (GOCI) data  

Science.gov (United States)

The historically massive bloom of the green macroalgae Ulva prolifera reported in June-August 2008 around the Qingdao, Yellow Sea, East China Sea and Japan coasts has recurred in a similar season and region. On June 13, 2011, around Qingdao, China, the world's first Geostationary Ocean Color Imager (GOCI) detected an enormous bloom of floating green algae, which originated from the nearshore Subei Bank, China. The large floating green algae patches were observed along and across the Yellow Sea and in the East China Sea during 2011 summer by various oceanic cruises. To detect the massive macroalgae blooms from space, we analyzed their spectral characteristics from in situ optical measurements and satellite-derived green algae spectra. An "Index of floating Green Algae for GOCI" (IGAG) was developed from the multiple spectral band ratios using three wavelengths (555, 660, 745 nm), which the spectral response of green algae reflected at 555, 745, and 865 nm and absorbed at 660 and 680 nm. The results were compared with those obtained by the normalized difference vegetation index (NDVI), enhanced vegetation index (EVI), and Korea Ocean Satellite Center (KOSC) approaches. An advantage of the IGAG method was that muted or subtle signals of floating green algae were enhanced and separated from surrounding complex water signals. Although maps of floating green algae derived by the other approaches delineated dense green algae, they were less sensitive to subtle (less dense) features and in cases of nearby cloudy or complex water conditions. The floating green algae maps from IGAG provided a more robust estimate of wide floating green algae blooms than those derived using NDVI, EVI, or KOSC approaches. The IGAG approach should be useful for tracing and monitoring changes in green algae blooms on regional and global scales.

Son, Young Baek; Min, Jee-Eun; Ryu, Joo-Hyung

2012-09-01

265

A surface complexation model of YREE sorption on Ulva lactuca in 0.05-5.0 M NaCl solutions  

Science.gov (United States)

We present distribution coefficients, log iKS, for the sorption of yttrium and the rare earth elements (YREEs) on BCR-279, a dehydrated tissue homogenate of a marine macroalga, Ulva lactuca, resembling materials featured in chemical engineering studies aimed at designing renewable biosorbents. Sorption experiments were conducted in NaCl solutions of different ionic strength (0.05, 0.5, and 5.0 M) at T = 25 °C over the pH range 2.7-8.5. Distribution coefficients based on separation of the dissolved and particulate phase by conventional filtration (3 kDa) using an existing pH-dependent model. Colloid-corrected values were renormalized to free-cation concentrations by accounting for YREE hydrolysis and chloride complexation. At each ionic strength, the pH dependence of the renormalized values is accurately described with a non-electrostatic surface complexation model (SCM) that incorporates YREE binding to three monoprotic functional groups, previously characterized by alkalimetric titration, as well as binding of YREE-hydroxide complexes (MOH2+) to the least acidic one (pKa ? 9.5). In non-linear regressions of the distribution coefficients as a function of pH, each pKa was fixed at its reported value, while stability constants of the four YREE surface complexes were used as adjustable parameters. Data for a single fresh U. lactuca specimen in 0.5 M NaCl show generally the same pH-dependent behavior but a lower degree of sorption and were excluded from the regressions. Good linear free-energy relations (LFERs) between stability constants of the YREE-acetate and YREE-hydroxide solution complex and surface complexes with the first and third functional group, respectively, support their prior tentative identifications as carboxyl and phenol. A similar confirmation for the second group is precluded by insufficient knowledge of the stability of YREE-phosphate complexes and a perceived lack of YREE binding in 0.05 M NaCl; this issue awaits further study. The results indicate that SCMs can be successfully applied to sorbents as daunting as marine organic matter. Despite remnant challenges, for instance resolving the contributions of individual groups to the aggregate sorption signal, our approach helps formalize seaweed’s avowed promise as an ideal biomonitor or biofilter of metal pollution in environments ranging from freshwaters to brines by uncovering what chemical mechanisms underlie its pronounced affinity for YREEs and other surface-reactive elements.

Zoll, Alison M.; Schijf, Johan

2012-11-01

266

Nutrient uptake efficiency of Gracilaria chilensis and Ulva lactuca in an IMTA system with the red abalone Haliotis rufescens / Eficiencia de absorción de nutrientes de Gracilaria chilensis y Ulva lactuca en un sistema multitrófico integrado con el abalón rojo Haliotis rufescens  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in spanish Se analizó la eficiencia de captación de nutrientes de Ulva lactuca y Gracilaria chilensis, cultivadas en estanques asociados a aguas de desecho proveniente de un cultivo del abalón rojo Haliotis rufescens. Los experimentos consideraron evaluar diferentes densidades de cultivo de algas (1200, 1900, [...] 2600 and 3200 g m-2) y tasas de recambio de agua (60, 80, 125 y 250 L h-1). Los resultados mostraron que tanto U. lactuca como G. chilensis fueron eficientes en la captación de nutrientes inorgánicos provenientes del cultivo de abalón, en todas las condiciones probadas, con remoción total de los nutrientes aportados por el cultivo. De la misma forma se realizó un experimento anual con U. lactuca, siendo cultivada con densidad de 1900 g m-2 y tasa de recambio de agua de 125 L h-1 para evaluar cambios estacionales en la eficiencia de captación de nutrientes, así como en la productividad y tasa de crecimiento de U. lactuca. Los resultados confirmaron una alta eficiencia de captación de U. lactuca durante todo el año, equivalente a la remoción del 100% del NH4, NO3 y PO4 aportado por el cultivo de abalón. La tasa de crecimiento y la productividad de U. lactuca presentó una marcada estacionalidad, incrementando de otoño al verano, variando de 0,5 ± 0,2 a 2,6 ± 0,2% d-1 y 10 ± 6,1 a 73,6 ± 8,4 g m-2 d-1, respectivamente. Se concluye que existen antecedentes suficientes que demuestran que es altamente posible cambiar la actividad tradicional de cultivo de abalón por un sistema integrado multitrófico, alcanzando externalidades ambientales positivas que incluyen U. lactuca como unidad de biofiltración. Abstract in english The current study examined the nutrient uptake efficiency of Ulva lactuca and Gracilaria chilensis cultivated in tanks associated with the wastewater of a land-based abalone culture. The experiments evaluated different seaweed stocking densities (1200, 1900, 2600, and 3200 g m-2) and water exchange [...] rates (60, 80, 125, and 250 L h-1). The results show that both U. lactuca and G. chilensis were efficient in capturing and removing all of the inorganic nutrients originating from the abalone cultivation for all of the tested conditions. Furthermore, an annual experiment was performed with U. lactuca, cultivated at a stocking density of 1900 g m-2 and at a water exchanged rate of 125 L h-1, in order to evaluate seasonal changes in the nutrient uptake efficiency, productivity, and growth rate associated with the wastewater of a land-based abalone culture. The results confirmed high uptake efficiency during the entire year, equivalent to a 100% removal of the NH4, NO3, and PO4 produced by the land-based abalone culture. The growth rate and productivity of U. lactuca presented a marked seasonality, increasing from fall until summer and varying from 0.5 ± 0.2% to 2.6 ± 0.2% d-1 and 10 ± 6.1% to 73.6 ± 8.4% g m-2 d-1 for sustainable growth rate and productivity, respectively. We conclude that there is sufficient evidence that demonstrates the high possibility of changing the traditional monoculture system of abalone in Chile, to a sustainable integrated multi-trophic aquaculture system, generating positive environmental externalities, including the use of U. lactuca as a biofiltration unit.

Juan, Macchiavello; Cristian, Bulboa.

267

Enzymatic regulation of photosynthetic and light-independent carbon fixation in Laminaria setchellii (Phaeophyta), Ulva lactuca (Chlorophyta) and Iridaea cordata (Rhodophyta) / Regulación enzimática de la fotosíntesis y la fijación de carbono en obscuridad por Laminaria setchellii (Phaeophyta), Ulva lactuca (Chlorophyta) e Iridaea cordata (Rhodophyta)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in spanish La incorporación de carbono en algas marinas se lleva a cabo mediante procesos fotosintéticos y no-fotosintéticos. Sin embargo, poco se sabe sobre la regulación bioquímica de estas rutas metabólicas en el tejido de algas marinas. En consecuencia, el objetivo de este estudio fue el de evaluar la dist [...] ribución de la carboxilación in vivo y relacionarlas a la actividad in vitro de ribulosa 1,5-bisfosfato carboxilasa/oxigenasa (RUBISCO), fosfoenolpiruvato carboxikinasa (PEPCK) y fosfoenolpiruvato carboxilasa (PEPC) en la Phaeophyta Laminaria setchellii, la Chlorophyta Ulva lactuca y la Rhodophyta Iridaea cordata. Los niveles de clorofila-a no variaron en U. lactuca e I. cordata. Sin embargo, los niveles de pigmentos fueron significativamente menores en la región meristemática de L. setchellii probablemente debido a una falta de diferenciación de los cloroplastos de esta región. De una manera similar, la fotosíntesis neta no varió en el talo de U. lactuca e I. cordata, mientras que se incrementó desde el estipe y el meristemo hacia la lámina de L. setchellii. En contraste con la fotosíntesis, la fijación de carbono en oscuridad fue significantemente mayor en la zona meristemática de L. setchellii sugiriendo un mecanismo para la compensación de la incorporación de carbono en tejido fotosintéticamente limitado. La actividad enzimática de RUBISCO y PEPCK se comportó de una manera similar a los procesos carboxilantes in vivo, indicando que la fotosíntesis y la fijación de carbono en oscuridad son reguladas por la actividad de las enzimas carboxilantes a lo largo del talo de L. setchellii Abstract in english Carbon is acquired through photosynthetic and non-photosynthetic processes in marine algae. However, little is known about the biochemical regulation of these metabolic pathways along the thallus of seaweeds. Consequently, the objective of this study was to assess the distribution of in vivo carboxy [...] lation pathways and to relate them to the in vitro activity of ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO), phosphoenolpyruvate carboxykinase (PEPCK), and phosphoenolpyruvate carboxylase (PEPC) in the Phaeophyte Laminaria setchellii, the Chlorophyte Ulva lactuca, and the Rhodophyte Iridaea cordata. Chlorophyll-a levels did not vary in U. lactuca and I. cordata. However, pigment levels were significantly lower in the meristematic region of L. setchellii probably as a result of a lack of differentiation of the chloroplasts in this region. Similarly, net photosynthesis did not vary in the thallus of U. lactuca and I. cordata, while it increased from the stipe and meristem towards the lamina of L. setchellii. In contrast to photosynthesis, light-independent carbon fixation rates were significantly greater in the meristematic region of L. setchellii suggesting a compensating mechanism for carbon incorporation in photosynthetically limited tissue. The activity of RUBISCO and PEPCK followed a pattern similar to that of in vivo carboxylation processes indicating that in vivo carbon assimilation is regulated by the activity of the carboxylating enzymes throughout the thallus of L. setchellii

ALEJANDRO, CABELLO-PASINI; RANDALL S., ALBERTE.

2001-06-01

268

Nutrient uptake efficiency of Gracilaria chilensis and Ulva lactuca in an IMTA system with the red abalone Haliotis rufescens / Eficiencia de absorción de nutrientes de Gracilaria chilensis y Ulva lactuca en un sistema multitrófico integrado con el abalón rojo Haliotis rufescens  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in spanish Se analizó la eficiencia de captación de nutrientes de Ulva lactuca y Gracilaria chilensis, cultivadas en estanques asociados a aguas de desecho proveniente de un cultivo del abalón rojo Haliotis rufescens. Los experimentos consideraron evaluar diferentes densidades de cultivo de algas (1200, 1900, [...] 2600 and 3200 g m-2) y tasas de recambio de agua (60, 80, 125 y 250 L h-1). Los resultados mostraron que tanto U. lactuca como G. chilensis fueron eficientes en la captación de nutrientes inorgánicos provenientes del cultivo de abalón, en todas las condiciones probadas, con remoción total de los nutrientes aportados por el cultivo. De la misma forma se realizó un experimento anual con U. lactuca, siendo cultivada con densidad de 1900 g m-2 y tasa de recambio de agua de 125 L h-1 para evaluar cambios estacionales en la eficiencia de captación de nutrientes, así como en la productividad y tasa de crecimiento de U. lactuca. Los resultados confirmaron una alta eficiencia de captación de U. lactuca durante todo el año, equivalente a la remoción del 100% del NH4, NO3 y PO4 aportado por el cultivo de abalón. La tasa de crecimiento y la productividad de U. lactuca presentó una marcada estacionalidad, incrementando de otoño al verano, variando de 0,5 ± 0,2 a 2,6 ± 0,2% d-1 y 10 ± 6,1 a 73,6 ± 8,4 g m-2 d-1, respectivamente. Se concluye que existen antecedentes suficientes que demuestran que es altamente posible cambiar la actividad tradicional de cultivo de abalón por un sistema integrado multitrófico, alcanzando externalidades ambientales positivas que incluyen U. lactuca como unidad de biofiltración. Abstract in english The current study examined the nutrient uptake efficiency of Ulva lactuca and Gracilaria chilensis cultivated in tanks associated with the wastewater of a land-based abalone culture. The experiments evaluated different seaweed stocking densities (1200, 1900, 2600, and 3200 g m-2) and water exchange [...] rates (60, 80, 125, and 250 L h-1). The results show that both U. lactuca and G. chilensis were efficient in capturing and removing all of the inorganic nutrients originating from the abalone cultivation for all of the tested conditions. Furthermore, an annual experiment was performed with U. lactuca, cultivated at a stocking density of 1900 g m-2 and at a water exchanged rate of 125 L h-1, in order to evaluate seasonal changes in the nutrient uptake efficiency, productivity, and growth rate associated with the wastewater of a land-based abalone culture. The results confirmed high uptake efficiency during the entire year, equivalent to a 100% removal of the NH4, NO3, and PO4 produced by the land-based abalone culture. The growth rate and productivity of U. lactuca presented a marked seasonality, increasing from fall until summer and varying from 0.5 ± 0.2% to 2.6 ± 0.2% d-1 and 10 ± 6.1% to 73.6 ± 8.4% g m-2 d-1 for sustainable growth rate and productivity, respectively. We conclude that there is sufficient evidence that demonstrates the high possibility of changing the traditional monoculture system of abalone in Chile, to a sustainable integrated multi-trophic aquaculture system, generating positive environmental externalities, including the use of U. lactuca as a biofiltration unit.

Juan, Macchiavello; Cristian, Bulboa.

2014-07-01

269

Enzymatic regulation of photosynthetic and light-independent carbon fixation in Laminaria setchellii (Phaeophyta, Ulva lactuca (Chlorophyta and Iridaea cordata (Rhodophyta Regulación enzimática de la fotosíntesis y la fijación de carbono en obscuridad por Laminaria setchellii (Phaeophyta, Ulva lactuca (Chlorophyta e Iridaea cordata (Rhodophyta  

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Full Text Available Carbon is acquired through photosynthetic and non-photosynthetic processes in marine algae. However, little is known about the biochemical regulation of these metabolic pathways along the thallus of seaweeds. Consequently, the objective of this study was to assess the distribution of in vivo carboxylation pathways and to relate them to the in vitro activity of ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO, phosphoenolpyruvate carboxykinase (PEPCK, and phosphoenolpyruvate carboxylase (PEPC in the Phaeophyte Laminaria setchellii, the Chlorophyte Ulva lactuca, and the Rhodophyte Iridaea cordata. Chlorophyll-a levels did not vary in U. lactuca and I. cordata. However, pigment levels were significantly lower in the meristematic region of L. setchellii probably as a result of a lack of differentiation of the chloroplasts in this region. Similarly, net photosynthesis did not vary in the thallus of U. lactuca and I. cordata, while it increased from the stipe and meristem towards the lamina of L. setchellii. In contrast to photosynthesis, light-independent carbon fixation rates were significantly greater in the meristematic region of L. setchellii suggesting a compensating mechanism for carbon incorporation in photosynthetically limited tissue. The activity of RUBISCO and PEPCK followed a pattern similar to that of in vivo carboxylation processes indicating that in vivo carbon assimilation is regulated by the activity of the carboxylating enzymes throughout the thallus of L. setchelliiLa incorporación de carbono en algas marinas se lleva a cabo mediante procesos fotosintéticos y no-fotosintéticos. Sin embargo, poco se sabe sobre la regulación bioquímica de estas rutas metabólicas en el tejido de algas marinas. En consecuencia, el objetivo de este estudio fue el de evaluar la distribución de la carboxilación in vivo y relacionarlas a la actividad in vitro de ribulosa 1,5-bisfosfato carboxilasa/oxigenasa (RUBISCO, fosfoenolpiruvato carboxikinasa (PEPCK y fosfoenolpiruvato carboxilasa (PEPC en la Phaeophyta Laminaria setchellii, la Chlorophyta Ulva lactuca y la Rhodophyta Iridaea cordata. Los niveles de clorofila-a no variaron en U. lactuca e I. cordata. Sin embargo, los niveles de pigmentos fueron significativamente menores en la región meristemática de L. setchellii probablemente debido a una falta de diferenciación de los cloroplastos de esta región. De una manera similar, la fotosíntesis neta no varió en el talo de U. lactuca e I. cordata, mientras que se incrementó desde el estipe y el meristemo hacia la lámina de L. setchellii. En contraste con la fotosíntesis, la fijación de carbono en oscuridad fue significantemente mayor en la zona meristemática de L. setchellii sugiriendo un mecanismo para la compensación de la incorporación de carbono en tejido fotosintéticamente limitado. La actividad enzimática de RUBISCO y PEPCK se comportó de una manera similar a los procesos carboxilantes in vivo, indicando que la fotosíntesis y la fijación de carbono en oscuridad son reguladas por la actividad de las enzimas carboxilantes a lo largo del talo de L. setchellii

ALEJANDRO CABELLO-PASINI

2001-06-01

270

Ecologia populacional dos Amphipoda (Crustacea) dos fitais de Caiobá, Matinhos, Paraná, Brasil / Population ecolocy of Amphipoda (Crustacea) from the phytals of Caiobá, Matinhos, Paraná, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese [...] Abstract in english Spalial and temporal density distributions of Amphipoda from the phytals of Caiobá are described. Air temperature oscillated from 16ºC (August and May) to 23ºC (March), surface water temperature from 17ºC (August) to 25ºC (March) and the salinity from 29.3‰ (May) to 32.8‰ (August). Two samples of 25 [...] cm² (for algae less than 5cm long), 100 cm² (for algae between 5-10cm long) and whole plants (for algae more than 10cm long) were removed with a spatula from the rocky surface at Caiobá Beach, in August/86, November/86, March/87 and May/87. After sorting, the algal substrata were weighted, their adsorption coefficient calculated and the sediment retained among the thallii weighted. The average distance between the branching was measured for all branched algae. The densities were calculated in relation to the weight of the algal substrate in grams. Eight phytals were considered: Ulva fasciata Delile, Padina gymnospora (Kútzing) Vickers, Sargassum cymosum Garth, Porphyra atropurpurea (Olivi) De Toni, Gelidium sp., Gymnogongrus griffithsiae (Turner) Martius, Pterocladia capillacea (Gmelin) Bornet &Thurel and Pterosiphonia pennata (Roth) Falkenberg, over which nine Amphipoda species live: Ampithoe ramondi Audouin, 1816, Cymadusa filosa Savigny, 1852, Elasmopus pectenicrus Bate, 1857, Hyale media Dana, 1857, Hyale sp.l, Jassa falcata Montagu, 1895 and Sunampithoe pelagica H. Milne-Edwards, 1830 (Gammaridea). Caprella danilevskii Czerniavski, 1861 and Caprella penantis Leach, 1814 (Caprellidea). Amphipoda densities ranged from 0.27 ind.g-1 to 45.68. ind.g-1. The broad-thallii algae Porphyra, Ulva and Padina harbored lower densities of Amphipoda, whereas those finely branched Pterocladia, Pterosiphonia and Gymnogongrus, the highest values and the less branched Sargassum and Gelidium, intermediate values. The high densities found in the finely branched algae had as main contribution the juvenile recruiting of most Amphipoda. The tide level might have influenced the temporal distribution of the Amphipoda density, due to the distinct time of air exposition in eaeh collection data. Most Amphipoda did not show specific algal substratum colonization: only Sunampithoe pelagica occurred solely in Sargassum. Four species occurred in different branched algae: J. falcata. S. pelagica. C. danilevskii and C. penantis. H. media had Sargassum, Pterocladia, Pterosiphonia and Gymnogongrus as the best algal substrata, whereas Caprellidea, the Pterocladia and Pterosiphonia phytals. High sediment weight in Padina was the main reason for high densities of Hyale sp.l in this phytal. The occurrence of males, females (including ovigerous ones) and juveniles of most Amphipoda species found in the present study indicates a complete life cycle whithin these phytals and corroborates with the assumption of the complexity of this marine coastal ecosystem.

Janete, Dubiaski-Silva; Setuko, Masunari.

271

Are anti-fouling effects in coralline algae species specific?  

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The crustose coralline algae are susceptible to be covered by other algae, which in turn can be affected by anti-fouling effects. In this study the hypothesis tested was that these algae can inhibit the growth of epiphytes in a species specific way. In the laboratory, propagules of Sargassum furcatum and Ulva fasciata were liberated and cultivated on pieces of coralline algae and slide covers (controls) and their survival and growth were compared. Spongites and Hydrolithon significantly inhib...

Alexandre Bigio Villas Bôas; Figueiredo, Marcia A. O.

2004-01-01

272

A Structural Modelling Study on Marine Sediments Toxicity  

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Quantitative structure-activity relationship models were obtained by applying the Molecular Descriptor Family approach to eight ordnance compounds with different toxicity on five marine species (arbacia punctulata, dinophilus gyrociliatus, sciaenops ocellatus, opossum shrimp, and ulva fasciata). The selection of the best among molecular descriptors generated and calculated from the ordnance compounds structures lead to accurate monovariate models. The resulting models obtained for six endpoin...

Bolboaca?„a?ƒ, Sorana D.; Lorentz Jäntschi

2008-01-01

273

EVALUATION OF GLYCOLIPIDS OF SOME EGYPTIAN MARINE ALGAE AS A SOURCE OF BIOACTIVE SUBSTANCES  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Glycolipids in five species of marine algae: two species of Rhodophyta (Laurencia popillose, Galaxoura cylindriea); and one species of Chlorophyta (Ulva fasciata), and two species of Phaeophyta (Dilophys fasciola, Taonia atomaria ) collected from Red and Mediterranean sea, respectively were extracted, purified on silica gel column chromatography and identified by liquid chromatography MS/MS. Total glycolipid contents (GL) (as % of total lipid) were found to be in ranges 10.9 to 28.7%. T. ato...

El Baroty Gamal S; El-Baz; Farouk K; Abd-Elmoein Ibtisam; Abd El Baky Hanaa H; Ali Mohamed M; Ibrahim Eman A

2011-01-01

274

Tratamiento de efluentes del cultivo de Litopenaeus vannamei mediante procesos de sedimentación, filtración y absorción Treatments of effluents from Litopenaeus vannamei shrimp cultures through sedimentation, filtration and absorption  

Digital Repository Infrastructure Vision for European Research (DRIVER)

La eficiencia de remoción de materia particulada y nutrientes disueltos de efluentes de estanques de reproductores de Litopenaeus vannamei fue evaluada a escala de laboratorio, empleando tratamientos de sedimentación, filtración por Crassostrea rhizophorae y absorción por Ulva fasciata. En cada tratamiento se empleó un tiempo de residencia hidráulica del efluente de seis horas. En la etapa de sedimentación se utilizaron estanques con 90 L del efluente. Para la ...

Roberto Ramos; Luis Vinatea; Julia Santos; Rejane Da Costa

2010-01-01

275

Inhibition of attachment of some fouling diatoms and settlement of Ulva lactuca zoospores by film-forming bacterium and their extracellular products isolated from biofouled substrata in Northern Chile  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in english The biofouling of surfaces submerged in the marine environment includes primary colonization of the substrate by microorganisms including bacteria, microalgae, and microscopic reproductive propagules of macroorganisms such as algal zoospores. The present study reports the evaluation of the inhibitor [...] y potential of biofilms and extracellular products (EP) of the indigenous bacterium Alteromonas sp strain Ni1-LEM on the settlement of marine biofouling such as: (i) eight marine benthic diatoms and (ii) zoospores of the alga Ulva lactuca, as well as the germination of these zoospores and was compared with reference strains with proven antifouling properties, Halomonas marina (ATCC 25374) and Pseudoalteromonas tunicata. Highest antifouling activity was found for the indigenous strain. In attempts to better define the chemical nature of the antifouling substance in the EP of the Alteromonas sp strain Ni1-LEM, the culture filtrates were tested for activity after heat treatment, enzymatic treatments, dialysis through semipermeable membranes, and separation into polar (aqueous) and non-polar (organic) fractions. The results suggested that the antifouling substance in the culture filtrates to be protein or peptide in nature, thermostable, hydrophilic, and equal to or greater than 3500 daltons in molecular size. Antifouling substances from bacteria may lead to the development of novel antifouling agents in the future.

Fernando, Silva-Aciares; Carlos, Riquelme.

2008-01-15

276

Are anti-fouling effects in coralline algae species specific?  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese As algas calcárias crostosas são susceptíveis ao recobrimento por outras algas, entretanto, estas podem ser afetadas por efeitos anti-incrustantes. Neste estudo foi testada a hipótese de que estas algas possam inibir o crescimento somente de algumas espécies de epífitas. No laboratório, propágulos d [...] e Sargassum furcatum e Ulva fasciata foram liberados e cultivados sobre pedaços de algas calcárias e lamínulas de microscopia (controle) e as suas sobrevivência e crescimento comparadas. Spongites e Hydrolithon inibiram significativamente o crescimento de U. fasciata, mas não de Sargassum. No campo, pedaços de três espécies de algas calcárias vivas, mortas e cópias destas em discos de massa epóxi foram fíxos na rocha. Após um mês as algas epífitas foram identificadas e sua massa seca quantificada. Lithophyllum não inibiu o crescimento das epífitas, em contraste Spongites e outra coralinácea indeterminada inibiram o crescimento de Enteromorpha spp., Ulva fasciata e Hincksia mitchelliae. Colpomenia sinuosa esteve sempre ausente sobre as crostas vivas, porém presente nos controles. Resultados demonstram que a relação epífita-hospedeiro depende das espécies que estejam interagindo. O desprendimento de células superficiais das crostas coralináceas aponta para um possível efeito físico anti-incrustante, não se excluindo o químico. Abstract in english The crustose coralline algae are susceptible to be covered by other algae, which in turn can be affected by anti-fouling effects. In this study the hypothesis tested was that these algae can inhibit the growth of epiphytes in a species specific way. In the laboratory, propagules of Sargassum furcatu [...] m and Ulva fasciata were liberated and cultivated on pieces of coralline algae and slide covers (controls) and their survival and growth were compared. Spongites and Hydrolithon significantly inhibited the growth of U. fasciata but not Sargassum. In the field, pieces of three species of live and dead coralline algae and their copies in epoxy putty discs were fixed on the rock. After one month epiphytic algae were identified and their dry mass quantified. Lithophyllum did not affect the epiphyte growth. In contrast Spongites and an unidentified coralline significantly inhibited the growth of Enteromorpha spp., Ulva fasciata and Hincksia mitchelliae. Colpomenia sinuosa was absent on all living crusts, but present on controls. Results show that the epiphyte-host relation depends on the species that are interacting. The sloughing of superficial cells of coralline crusts points to the possible action of physical anti-fouling effect, though a chemical one is not rejected.

Alexandre Bigio, Villas Bôas; Marcia A. de O., Figueiredo.

2004-03-01

277

Are anti-fouling effects in coralline algae species specific?  

Directory of Open Access Journals (Sweden)

Full Text Available The crustose coralline algae are susceptible to be covered by other algae, which in turn can be affected by anti-fouling effects. In this study the hypothesis tested was that these algae can inhibit the growth of epiphytes in a species specific way. In the laboratory, propagules of Sargassum furcatum and Ulva fasciata were liberated and cultivated on pieces of coralline algae and slide covers (controls and their survival and growth were compared. Spongites and Hydrolithon significantly inhibited the growth of U. fasciata but not Sargassum. In the field, pieces of three species of live and dead coralline algae and their copies in epoxy putty discs were fixed on the rock. After one month epiphytic algae were identified and their dry mass quantified. Lithophyllum did not affect the epiphyte growth. In contrast Spongites and an unidentified coralline significantly inhibited the growth of Enteromorpha spp., Ulva fasciata and Hincksia mitchelliae. Colpomenia sinuosa was absent on all living crusts, but present on controls. Results show that the epiphyte-host relation depends on the species that are interacting. The sloughing of superficial cells of coralline crusts points to the possible action of physical anti-fouling effect, though a chemical one is not rejected.As algas calcárias crostosas são susceptíveis ao recobrimento por outras algas, entretanto, estas podem ser afetadas por efeitos anti-incrustantes. Neste estudo foi testada a hipótese de que estas algas possam inibir o crescimento somente de algumas espécies de epífitas. No laboratório, propágulos de Sargassum furcatum e Ulva fasciata foram liberados e cultivados sobre pedaços de algas calcárias e lamínulas de microscopia (controle e as suas sobrevivência e crescimento comparadas. Spongites e Hydrolithon inibiram significativamente o crescimento de U. fasciata, mas não de Sargassum. No campo, pedaços de três espécies de algas calcárias vivas, mortas e cópias destas em discos de massa epóxi foram fíxos na rocha. Após um mês as algas epífitas foram identificadas e sua massa seca quantificada. Lithophyllum não inibiu o crescimento das epífitas, em contraste Spongites e outra coralinácea indeterminada inibiram o crescimento de Enteromorpha spp., Ulva fasciata e Hincksia mitchelliae. Colpomenia sinuosa esteve sempre ausente sobre as crostas vivas, porém presente nos controles. Resultados demonstram que a relação epífita-hospedeiro depende das espécies que estejam interagindo. O desprendimento de células superficiais das crostas coralináceas aponta para um possível efeito físico anti-incrustante, não se excluindo o químico.

Alexandre Bigio Villas Bôas

2004-03-01

278

Novel insight into the copper-ligand geometry in the crystal structure of Ulva pertusa plastocyanin at 1.6-A resolution. Structural basis for regulation of the copper site by residue 88.  

Science.gov (United States)

The crystal structure of plastocyanin from a green alga, Ulva pertusa, has been determined at 1.6-A resolution. At its copper site, U. pertusa plastocyanin has a distorted tetrahedral coordination geometry similar to other plastocyanins. In comparison with structures of plastocyanins reported formerly, a Cu(II)-Sdelta(Met92) bond distance (2.69 A) is shorter by about 0.2 A and a Cu(II)-Sgamma(Cys84) distance is longer by less than 0.1 A in U. pertusa plastocyanin. These subtle but significant differences are caused by the structural change at a His-Met loop (His87-Met92) due to an absence of a O(Asp85)-Ogamma(Ser88) hydrogen bond which is found in Enteromorpha prolifera plastocyanin. In addition, poplar and Chlamydomonas reinhardtii plastocyanins with a glutamine at residue 88 have a weak cation-pi interaction with Tyr83. This interaction lengthens the Cu(II)-Sdelta(Met92) bond of poplar and C. reinhardtii plastocyanins by 0.14 and 0.20 A, respectively. As a result of structural differences, U. pertusa plastocyanin has a less distorted geometry than the other plastocyanins. Thus, the cupric geometry is finely tuned by the interactions between residues 85 and 88 and between residues 83 and 88. This result implies that the copper site is more flexible than reported formerly and that the rack mechanism would be preferable to the entatic theory. The His-Met loop may regulate the electron transfer rate within the complex between plastocyanin and cytochrome f. PMID:9933621

Shibata, N; Inoue, T; Nagano, C; Nishio, N; Kohzuma, T; Onodera, K; Yoshizaki, F; Sugimura, Y; Kai, Y

1999-02-12

279

Influence of microhabitat on seedling survival and growth of the mediterranean seagrass posidonia oceanica (l.) Delile  

Science.gov (United States)

Early life history phases are crucial stages limiting species distribution and abundance, thus influencing assemblage composition in marine benthic environments. In seagrass systems the period between seed germination and establishment is one of the most vulnerable phases for plant development. This study analyzes the influence of microhabitat structure, in terms of substrate nature and algal canopy, on the persistence and growth over two years of seedlings of Posidonia oceanica, the dominant Mediterranean seagrass. Long time persistence of seedlings only occurred on microhabitats providing vegetated rocky substrates, with a maximum value of 81% on rock covered by Cystoseira spp. No seedling was found on unvegetated sand and gravel after the first year. Seedling growth resulted increased on rock covered by Halopteris spp. and Dilophus spp. than on rock covered by Cystoseira spp. Results suggest that high canopy onto a stable substrate enhances seedling persistence, probably because these allow the best anchorage by roots while hampering water flow. In contrast, turf algal cover promotes better seedling growth, possibly through higher light irradiance and nutrient availability. Our findings support the view that the understanding of the factors controlling early life processes is a necessary prerequisite for the comprehension of seagrass species distribution patterns, colonization and recovery potentials, which, in turn, can guide sound strategies for seagrass management and restoration.

Alagna, Adriana; Fernández, Tomás Vega; Terlizzi, Antonio; Badalamenti, Fabio

2013-03-01

280

A NOVEL ACYLATED FLAVONOIDIC GLYCOSIDE FROM THE WOOD OF CULTIVATED ACACIA NILOTICA (L. WILLD. EX. DELILE.  

Directory of Open Access Journals (Sweden)

Full Text Available Acacia is a fast-growing plant that has high potential in commercial plantations in tropical areas. It is already being grown as a plantation crop for building and industrial raw materials, as well as for reforestation of difficult sites. Extensive cultivation of this promising tree would enrich the natural resources besides being useful for industrial raw material, waste land management, and afforestation. In addition, Acacia exhibits extensive medicinal values. In view of the medicinal importance of Acacia nilotica and the therapeutic utility of flavonoids, an attempt has been made to isolate novel flavonoids from the wood of cultivated A. nilotica. The extraction of crude ethanol extract from the A. nilotica wood was followed by fractionation with chloroform, ethyl acetate, and methanol in increasing order of polarity of the solvent. The mixed ethyl acetate and methanol extract afforded three pure compounds through column chromtomatography and fractional crystallization. Among the isolated phenolic compounds, a new acylated flavonoidic glycoside, tricin-4?-O-?-(6??-hydroxycinnamic-glucoside (1 was isolated from the wood of A. nilotica together with two known compounds, gallic acid and apigenin. Their structures were established by chemical evidence, spectroscopic techniques (FT-IR, 1H-NMR, 13C-NMR, HSQC, HMBC, and ESI-MS, and by comparison with already existing spectroscopic data. The yield of novel tricin glucoside showed that it make up to 0.0786% of mixed ethyl acetate and acetone extract.

Zakia Khanam

2011-07-01

 
 
 
 
281

Phenology of Posidonia Oceanica (Linneaus Delile in the West Coast of Algeria  

Directory of Open Access Journals (Sweden)

Full Text Available The Posidonia oceanica seagrass in the western coast of Algeria on the subject of annual monitoring from November 2008 to November 2009.Two sites are selected and each are represented by a station located 10meters deep. The first is Cap Carbon which is polluted site subject to various major releases, and the second one is Ain Franin considered as a reference site relatively far from sources of pollution other than pollution considered sporadic during the summer where there is a rare attendance of holidaymakers. A study of the phenology of Posidonia oceanica meadows, on the leaf biometry, the coefficient "A" and the surface shows that these parameters are strongly influenced by seasonal factors and not by the depth as our samples were made at same depth.

F. Chahrour

2013-01-01

282

Energy Production from Marine Biomass (Ulva lactuca)  

DEFF Research Database (Denmark)

The background for this research activity is that the 2020 goals for reduction of the CO2 emissions to the atmosphere are so challenging that exorbitant amounts of biomass and other renewable sources of energy must be mobilised in order to – maybe – fulfil the ambitious 2020 goals. The macroalgae is an unexploited, not researched, not developed source of biomass and is at the same time an enormous resource by mass. It is therefore obvious to look into this vast biomass resource and by this report give some of the first suggestions of how this new and promising biomass resource can be exploited.

Nikolaisen, Lars; Daugbjerg Jensen, Peter

2011-01-01

283

Are metals of antifouling paints transferred to marine biota?  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Because of its high toxicity, TBT (trybutiltin) was banned since 2003, which resulted in a greater re-use of Cu as based-biocide in antifouling paints (AFP). The aim of this work is to determine if metals form of AFP are transferred to benthic organisms from Guanabara Bay (GB) (Rio de Janeiro, Brazil). Metal concentrations were measured in two main fouling algae species Ulva flexuosa and U. fasciata and one isopod species, Sphaeroma serratum, in two GB marinas areas from sites with artificial...

Paradas, Wladimir C.; Amado Filho, Gilberto M.

2007-01-01

284

A Structural Modelling Study on Marine Sediments Toxicity  

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Full Text Available Quantitative structure-activity relationship models were obtained by applying the Molecular Descriptor Family approach to eight ordnance compounds with different toxicity on five marine species (arbacia punctulata, dinophilus gyrociliatus, sciaenops ocellatus, opossum shrimp, and ulva fasciata. The selection of the best among molecular descriptors generated and calculated from the ordnance compounds structures lead to accurate monovariate models. The resulting models obtained for six endpoints proved to be accurate in estimation (the squared correlation coefficient varied from 0.8186 to 0.9997 and prediction (the correlation coefficient obtained in leave-one-out analysis varied from 0.7263 to 0.9984.

Sorana D. Bolboacă

2008-06-01

285

Feeding habits of Hyale media (Dana, 1853) (Crustacea-Amphipoda)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english Feeding of males and females of the Gammaridea Hyate media at mature and immature stages were tested in laboratory experiments. Macro and microscopic algae as well as dead or alive animals were utilized as food. This gammarid is omnivorous, feeding by predation, scavenging, browsing and scraping. Fe [...] eding behaviour was discontinuous. Padina vickersiae was more utilized in winter and Ulva fasciata in summer. The feeding activity of all the animals showed great variability in relation to the type of food. Higher temperatures probably account for the higher consumption observed in summer.

Airton Santo, Tararam; Yoko, Wakabara; Hilda de Sousa Lima, Mesquita.

286

Feeding habits of Hyale media (Dana, 1853 (Crustacea-Amphipoda  

Directory of Open Access Journals (Sweden)

Full Text Available Feeding of males and females of the Gammaridea Hyate media at mature and immature stages were tested in laboratory experiments. Macro and microscopic algae as well as dead or alive animals were utilized as food. This gammarid is omnivorous, feeding by predation, scavenging, browsing and scraping. Feeding behaviour was discontinuous. Padina vickersiae was more utilized in winter and Ulva fasciata in summer. The feeding activity of all the animals showed great variability in relation to the type of food. Higher temperatures probably account for the higher consumption observed in summer.

Airton Santo Tararam

1985-01-01

287

Active fractions from four species of marine algae  

Scientific Electronic Library Online (English)

Full Text Available SciELO Argentina | Language: English Abstract in english A bioassay-directed is utilized to detect substances with biological activity from Gracilaria tikvahiae, Ulva lactuca, Ulva fasciata and Sargassum fluitans. In a preliminary assessment, polar and non polar extracts of four species of marine protoctist form were screened for antibacterial and antifun [...] gal properties against seven microorganisms by the diffusion method, non polar extracts of Sargassumfluitans,and polar extracts of Gracilaria tikvahiae inhibited the growth of more than four microorganisms. Extracts were separated using chromatography column and fractions were tested againstStapylococcusaureus andCandida albicans. The eighty fraction of petroleum ether of S. fluitans exhibited high activity against C.albicans, MIC 0.16 µg/mL.

MA, Oranday; MJ, Verde; SJ, Martínez-Lozano; NH, Waksman.

2004-12-01

288

Actividad fotosintética y asociación bacteriana en semillas de la fanerógama marina Posidonia oceanica (L.) Delile = Photosynthetic activity and bacterial association in seeds of the seagrass Posidonia oceanica (L.) Delile.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

La semilla de Posidonia oceanica es de color verde y al desprenderse del fruto queda libre en el mar en contacto con microorganismos marinos. Por estas dos razones nuestras hipótesis fueron confirmar si La semilla de Posidonia oceanica, y dos parientes australianas: Posidonia australis y Posidonia sinuosa tienen actividad fotosintética y si la semilla crea alguna relación con microorganismos marinos, (Marinomonas posidonica y Marinomonas mediterranea.). Nuestros resultados concluyeron que ...

Celdra?n Sabater, Manuel David

2012-01-01

289

El subestrat algal de l'herbei de Posidonia oceanica (L.) Delile de Cala Gat (NE de Mallorca)  

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Algal undergrowth of the Posidonia oceanica meadow in Cala Gat (NE of Majorca, Balearic Islands) The floristic composition of the Posidonia oceanica beds in Cala Gat is studied . The dominance of Melobesiae and Sphacelaria cirrosa is remarked . Records of Gelidiella antipai M. Celan and Lophosiphonia scopulorum (Harvey) Womersley are new to the Balearic Islands.

Ballesteros, Enric

1984-01-01

290

Assessment of the perception of Majorcan society of the ecological and economical values of Posidonia oceanica (L. Delile) seagrass beds.  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The perception of the Majorcan society of the ecological functions and services provided by Posidonia oceanica seagrass beds and their importance to the economy of the island was assessed by (1) the analysis of the cultural frameworks of the news related to this species published in the main daily Majorcan newspapers from October 2001 to August 2003, (2) a review of the actions performed by public and private institutions, and socio-economic and social groups of Mallorca with an interest and/...

Medina-pons, Francisco Javier; Terrados, Jorge; Ta?bara Villalba, David

2004-01-01

291

The performance, application and integration of various seabed classification systems suitable for mapping Posidonia oceanica (L.) Delile meadows.  

Science.gov (United States)

In the context of current global environmental changes, mapping and monitoring seagrass meadows have become highly important for management and preservation of coastal zone ecosystems. The purpose of this research was to determine the numerical precision of various cost-effective benthic habitat mapping techniques and their suitability for mapping and monitoring of Posidonia oceanica meadows in the Croatian Adriatic. We selected ultra-high resolution aerial imagery, single-beam echo sounder (SBES) seabed classification system from Quester Tangent Co. (QTC), and surface based underwater videography as affordable, non-destructive and simple to use systems for data acquisition. The ultra-high resolution digital imagery was capable of detecting P. oceanica meadows up to 4m depth with 94% accuracy, from 4m to 12.5m depth the accuracy dropped to app. 76%, and from 12.5 to 20 m the system was only capable of distinguishing seabed biota from substrata, though with 97% accuracy. The results of the QTC system showed over 90% detection accuracy for Cymodocea nodosa covered seabed, excellent separation capabilities (>92%) of different sediment types (slightly gravelly sand, gravelly muddy sand and slightly gravelly muddy sand) and reasonable accuracy for mapping underwater vegetation regardless of the bathymetric span. The system proved incapable of separating P. oceanica from dense macroalgae on the same type of substratum. Surface-based underwater videography demonstrated great potential for estimating P. oceanica cover in a sampled region using either a single human rater or a computer estimate. The consistency between two human scorers in evaluating P. oceanica bottom coverage was near perfect (>98%) and high between digital and human scorers (80%). The results indicate that although the selected systems are suitable for mapping seagrasses, they all display limitations in either detection accuracy or spatial coverage, which leads to a conclusion that suitable system integration is essential for producing high quality seagrass spatial distribution maps. PMID:24157476

Puhr, Kristian; Schultz, Stewart; Pikelj, Kristina; Petricioli, Donat; Bakran-Petricioli, Tatjana

2014-02-01

292

An unexplored sedimentary record for the study of environmental change in mediterranean coastal environments: Posidonia oceanica (L.) Delile peats  

International Nuclear Information System (INIS)

Information on seagrass paleo-ecology is very scarce because detailed seagrass paleorecords are virtually lacking. The endemic Mediterranean seagrass Posidonia oceanica conjugates two unusual features that allow the reconstruction of the past history of the plant at two different time scales. On the one hand, the study of the leaf sheaths that remain attached to the rhizomes after leaf abcision (lepidochronology), allows to differentiate up to 30 yearly cycles. On the other hand, radiocarbon dating of peat-like deposits derived from Posidonia oceanica rhizomes and roots ('mattes'), reveals a chronological organic record of the plant spanning several thousands of years. Changes in the isotopic signature (?13C) of the sheaths along Posidonia rhizomes from a meadow off Medes Islands (NW Mediterranean, Spain), were highly correlated with changes in annual leave production and with water transparency. These relationships and the isotopic analysis of sheath debris from several Posidonia peats along the Spanish Mediterranean coast are used to make some preliminary inferences about long-term meadow history. Several phenomena potentially making difficult the interpretation of the information contained in Posidonia peats are critically discussed. It is concluded that a detailed study of P. oceanica peats will open new vistas in Mediterranean paleo-ecological and paleo-environmental research (author)

293

Relationship of non-protein thiol pools and accumulated Cd or Hg in the marine macrophyte Posidonia oceanica (L.) Delile  

Energy Technology Data Exchange (ETDEWEB)

The accumulation of cadmium or mercury and the effect of these elements on the levels of non-protein thiols in the blades of the marine macrophyte Posidonia oceanica were investigated. A significant accumulation of cadmium or mercury, dependent on metal concentration supplied, was observed in metal-treated blades. In the blades treated either with cadmium or mercury, a significant increase in the levels of non-protein thiols (other than glutathione) and a marked depletion of the reduced glutathione content as a function of the metal, exposure time and metal concentration supplied were found. This investigation provides first experimental report on the relationship between non-protein thiol pools and accumulated cadmium or mercury in P. oceanica.

Maserti, B.E. [National Research Council (CNR), Institute of Biophysics, Area della Ricerca CNR, Via Moruzzi 1, 56125 Pisa (Italy)]. E-mail: bianca.elena.maserti@pi.ibf.cnr.it; Ferrillo, V. [National Research Council (CNR), Institute of Biophysics, Area della Ricerca CNR, Via Moruzzi 1, 56125 Pisa (Italy); University of Pisa, Department of Agricultural Plant, Section of Genetics, Via Matteotti 1, 56100 Pisa (Italy); Avdis, O. [National Centre for Marine Research (N.C.M.R.), 16604 Hellenion (Greece); Nesti, U. [National Research Council (CNR), Institute of Biophysics, Area della Ricerca CNR, Via Moruzzi 1, 56125 Pisa (Italy); Central Institute for Marine Research (I.C.R.A.M.), Via di Casalotti, 300-00166 Rome (Italy); Di Garbo, A. [National Research Council (CNR), Institute of Biophysics, Area della Ricerca CNR, Via Moruzzi 1, 56125 Pisa (Italy); Catsiki, A. [National Centre for Marine Research (N.C.M.R.), 16604 Hellenion (Greece); Maestrini, P.L. [University of Pisa, Department of Agricultural Plant, Section of Genetics, Via Matteotti 1, 56100 Pisa (Italy)

2005-11-10

294

Relationship of non-protein thiol pools and accumulated Cd or Hg in the marine macrophyte Posidonia oceanica (L.) Delile  

International Nuclear Information System (INIS)

The accumulation of cadmium or mercury and the effect of these elements on the levels of non-protein thiols in the blades of the marine macrophyte Posidonia oceanica were investigated. A significant accumulation of cadmium or mercury, dependent on metal concentration supplied, was observed in metal-treated blades. In the blades treated either with cadmium or mercury, a significant increase in the levels of non-protein thiols (other than glutathione) and a marked depletion of the reduced glutathione content as a function of the metal, exposure time and metal concentration supplied were found. This investigation provides first experimental report on the relationship between non-protein thiol pools and accumulated cadmium or mercury in P. oceanica

295

Modelling Zostera marina and Ulva spp. in a coastal lagoon  

Digital Repository Infrastructure Vision for European Research (DRIVER)

We have implemented new modules of seagrass and macroalgae in the European Regional Seas Ecosystem Model (ERSEM). The modules were tested using a version of ERSEM coupled with the General Ocean Turbulence Model (GOTM) in San Quintin Bay (SQB), a coastal lagoon in Baja California, Mexico. As we are working in a region where horizontal advective transport of nutrients is important, we have included the horizontal nutrient gradients which result in nutrient advection when combined with the local...

Aveytua-alcazar, Leslie; Camacho-ibar, Victor F.; Souza, Alejandro J.; Allen, J. Icarus; Torres, Ricardo

2008-01-01

296

Identification of the toxic constituents of sediments in a Brazilian subtropical estuary.  

Science.gov (United States)

Phase 1 of the TIE method was applied to samples of elutriates from sediments of the Itajaí-Açu estuary and adjacent coastal region in southern Brazil. Embryo-larval toxicity assays were used with the sea urchin Arbacia lixula in samples of raw elutriate, and treated with Ulva fasciata, EDTA and sodium thiosulfate. Inside the estuary, ammonia was responsible for more than 40% of the toxicity in both the dredged and undredged regions. A toxicity gradient was observed, between the estuary and the coastal region, with an increase in the importance of metals for the latter. Temporally, there is strong evidence of the influence of dredging and disposal of sediments in the contamination of the coastal dumping site. The results indicating that this area presents limitations in its saturation capacity. Chemical analysis indicated the metal Cu is probably responsible for the toxicity of the sediments observed, without the interference of ammonia. PMID:24503379

Poleza, Fernanda; Souza, Rafael C; Resgalla, Charrid

2014-03-15

297

Tropical Atlantic marine macroalgae with bioactivity against virulent and antibiotic resistant Vibrio / Macroalgas marinas tropicales atlánticas con actividad biológica contra vibrios virulentos y resistentes a antimicrobianos  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in spanish Se investigó la actividad antibacteriana de los extractos de etanol, metanol, hexano y acetona de las macroalgas Padina gymnospora (PG), Hypnea musciformes (HM), Ulva fasciata (UF) y Caulerpa prolifera (CP). Se utilizó el método de difusión en disco para evaluar el efecto antimicrobiano de las algas [...] contra cepas patrón de Vibrio parahaemolyticus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa y Salmonella enterica y cinco cepas virulentas y resistentes a los antimicrobianos: V. brasiliensis, V. xuii y V. navarrensis (aislado de la hemolinfa de Litopenaeus vannamei). Los extractos de etanol de PG y HM inhibieron todas las cepas de Vibrio. E. coli y P. aeruginosa solo eran susceptibles a los extractos de etanol de PG. Entre los extractos de metanol, solo UF fue bioactivo, inhibiendo V. navarrensis. El efecto antibacteriano de los extractos de etanol de PG, HM y UF contra bacterias virulentas y resistentes a los antimicrobianos sugiere que estas especies de macroalgas constituyen una fuente potencial de compuestos bioactivos. Abstract in english The antibacterial activity of ethanol, methanol, hexane and acetone-based extracts of the macroalgae Padina gymnospora (PG), Hypnea musciformes (HM), Ulva fasciata (UF) and Caulerpa prolifera (CP) was investigated. The disk diffusion method was used to evaluate the algae antimicrobial effect against [...] standard strains of Vibrio parahaemolyticus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella enterica and five virulent antibiotic-resistant strains of V. brasiliensis, V. xuii and V. navarrensis (isolated from the hemolymph of Litopenaeus vannamei). Ethanol extracts of PG and HM inhibited all Vibrio strains. E. coli and P. aeruginosa were only susceptible to ethanol extracts of PG. Among the methanol extracts, only UF was bioactive, inhibiting V. navarrensis. The observed inhibitory effect of ethanol extracts of PG, HM and UF against virulent antibiotic-resistant bacteria suggests these macroalgal species constitute a potential source of bioactive compounds.

Giselle Cristina, Silva; Renata, Albuquerque-Costa; Jackson R, Oliveira-Peixoto; Fernando E, Pessoa-Nascimento; Pedro B, de Macedo-Carneiro; Regine H, Silva dos Fernandes-Vieira.

2013-03-01

298

Tropical Atlantic marine macroalgae with bioactivity against virulent and antibiotic resistant Vibrio / Macroalgas marinas tropicales atlánticas con actividad biológica contra vibrios virulentos y resistentes a antimicrobianos  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in spanish Se investigó la actividad antibacteriana de los extractos de etanol, metanol, hexano y acetona de las macroalgas Padina gymnospora (PG), Hypnea musciformes (HM), Ulva fasciata (UF) y Caulerpa prolifera (CP). Se utilizó el método de difusión en disco para evaluar el efecto antimicrobiano de las algas [...] contra cepas patrón de Vibrio parahaemolyticus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa y Salmonella enterica y cinco cepas virulentas y resistentes a los antimicrobianos: V. brasiliensis, V. xuii y V. navarrensis (aislado de la hemolinfa de Litopenaeus vannamei). Los extractos de etanol de PG y HM inhibieron todas las cepas de Vibrio. E. coli y P. aeruginosa solo eran susceptibles a los extractos de etanol de PG. Entre los extractos de metanol, solo UF fue bioactivo, inhibiendo V. navarrensis. El efecto antibacteriano de los extractos de etanol de PG, HM y UF contra bacterias virulentas y resistentes a los antimicrobianos sugiere que estas especies de macroalgas constituyen una fuente potencial de compuestos bioactivos. Abstract in english The antibacterial activity of ethanol, methanol, hexane and acetone-based extracts of the macroalgae Padina gymnospora (PG), Hypnea musciformes (HM), Ulva fasciata (UF) and Caulerpa prolifera (CP) was investigated. The disk diffusion method was used to evaluate the algae antimicrobial effect against [...] standard strains of Vibrio parahaemolyticus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella enterica and five virulent antibiotic-resistant strains of V. brasiliensis, V. xuii and V. navarrensis (isolated from the hemolymph of Litopenaeus vannamei). Ethanol extracts of PG and HM inhibited all Vibrio strains. E. coli and P. aeruginosa were only susceptible to ethanol extracts of PG. Among the methanol extracts, only UF was bioactive, inhibiting V. navarrensis. The observed inhibitory effect of ethanol extracts of PG, HM and UF against virulent antibiotic-resistant bacteria suggests these macroalgal species constitute a potential source of bioactive compounds.

Giselle Cristina, Silva; Renata, Albuquerque-Costa; Jackson R, Oliveira-Peixoto; Fernando E, Pessoa-Nascimento; Pedro B, de Macedo-Carneiro; Regine H, Silva dos Fernandes-Vieira.

299

Atividade antioxidante in vitro de extratos de algumas algas verdes (Chlorophyta do litoral catarinense (Brasil Antioxidant in vitro activity of extracts of some green seaweed (Chlorophyta from southern Brazilian coast  

Directory of Open Access Journals (Sweden)

Full Text Available O efeito antioxidante de quatro espécies de algas marinhas do filo Chlorophyta (Codium decorticatum, Enteromorpha intestinalis, Ulva fasciata e Chaetomorpha anteninna foi avaliado através da inibição da peroxidação do ácido linoléico em emulsão. Os extratos etéreos e metanólicos, na concentração de 0,01%, foram obtidos por extração seqüencial das biomassas secas. As espécies mais efetivas sobre a peroxidação lipídica foram Enteromorpha intestinalis e Chaetomorpha anteninna, com porcentagens de inibição acima de 70%. A habilidade dos extratos metanólicos para seqüestrar o peróxido de hidrogênio foi avaliada, obtendo-se valores médios para porcentagens de captura entre 1,26% e 20,01%. Além disto, quantificaram-se os teores de clorofila a, carotenóides totais e compostos fenólicos totais nas biomassas algais. Os resultados indicam que as algas verdes estudadas são uma fonte promissora de compostos biologicamente ativos com propriedades antioxidantes.The antioxidant activity of four species of green seaweeds of the phylo Chlorophyta (Codium decorticatum, Enteromorpha intestinalis, Ulva fasciata and Chaetomorpha anteninna collected at the seacoast of the State of Santa Catarina, Brazil, was evaluated by means of the inhibition of peroxidation of linoleic acid converted into emulsion. Both ethereal and methanolic extracts were obtained from dried biomasses by sequential extraction procedures in concentrations of 0.01%. The most efficient species towards lipid peroxidation were E. intestinalis and C. anteninna with inhibition yields above 70% .The capacity of methanolic extracts to quench hydrogen peroxide was also estimated. Mean values varied from 1.26 to 20.01%. Chlorophylls a, total carotenoids and phenolic compounds were also quantified in the biomasses. Results indicated that studied green seaweeds are a very promising source of biologically active compounds with antioxidant properties.

Melissa dos Santos Raymundo

2004-12-01

300

Aplicação foliar de tratamentos para o controle do míldio e da podridão-de-escamas de bulbos de cebola / Foliar spray of treatments in the control of downy mildew and bulb rot in onion  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese Em experimento de campo, avaliou-se o efeito da aplicação foliar de tratamentos para o controle do míldio (Peronospora destructor) e da podridão de bulbos (Burkholderia cepacia) de cebola: testemunha, clorotalonil/metalaxyl + clorotalonil, fosfito de potássio, fertilizante foliar (03-00-16, N-P-K), [...] calda bordalesa, calda bordalesa/fosfito de potássio, acibenzolar-S-methyl, pulverizados semanalmente; extrato de alga (Ulva fasciata) e ulvana, aplicados a cada 7, 14 e 21 dias. Somente a pulverização semanal com fungicidas sintéticos ou com o fertilizante (03-00-16; 400 mL de p.c./100 L) foi capaz de reduzir significativamente a severidade do míldio, em 60 ou 23%, respectivamente, em relação à testemunha não pulverizada, sem aumentar o rendimento de bulbos. O tratamento com fertilizantes ricos em potássio resultou em maior incidência da podridão de bulbos armazenados por cinco meses. O conteúdo de açúcares solúveis e incidência da podridão de bulbos de cebola foram correlacionados significativamente (-0,629, p Abstract in english A field experiment was carried out to evaluate the effect of foliar sprays with the following treatments on the downy mildew (Peronospora destructor) and bulb rot (Burkholderia cepacia) in onions: non-treated control, fungicide chlorotalonil/metalaxyl + chlorotalonil, potassium phosphite, foliar fer [...] tilizer (03-00-16, N-P-K), bordeaux mixture, bordeaux mixture/potassium phosphite, acibenzolar-S-methyl weekly applied; extract of alga Ulva fasciata and ulvan sprayed every 7, 14 and 21 days. Only the weekly spraying of fungicides and fertilizer (03-00-16, 400 mL/100 L) significantly reduced the mildew severity by 60 and 23%, respectively, but did not increase the bulb yield. The foliar application of potassium rich fertilizers resulted in a higher incidence of rotten bulbs after 5 months in storage. Soluble sugar content and rot incidence of onion bulbs were significantly correlated (-0,629, p

João Américo, Wordell Filho; Daniel A, Martins; Marciel J., Stadnik.

2007-12-01

 
 
 
 
301

Aplicação foliar de tratamentos para o controle do míldio e da podridão-de-escamas de bulbos de cebola Foliar spray of treatments in the control of downy mildew and bulb rot in onion  

Directory of Open Access Journals (Sweden)

Full Text Available Em experimento de campo, avaliou-se o efeito da aplicação foliar de tratamentos para o controle do míldio (Peronospora destructor e da podridão de bulbos (Burkholderia cepacia de cebola: testemunha, clorotalonil/metalaxyl + clorotalonil, fosfito de potássio, fertilizante foliar (03-00-16, N-P-K, calda bordalesa, calda bordalesa/fosfito de potássio, acibenzolar-S-methyl, pulverizados semanalmente; extrato de alga (Ulva fasciata e ulvana, aplicados a cada 7, 14 e 21 dias. Somente a pulverização semanal com fungicidas sintéticos ou com o fertilizante (03-00-16; 400 mL de p.c./100 L foi capaz de reduzir significativamente a severidade do míldio, em 60 ou 23%, respectivamente, em relação à testemunha não pulverizada, sem aumentar o rendimento de bulbos. O tratamento com fertilizantes ricos em potássio resultou em maior incidência da podridão de bulbos armazenados por cinco meses. O conteúdo de açúcares solúveis e incidência da podridão de bulbos de cebola foram correlacionados significativamente (-0,629, p A field experiment was carried out to evaluate the effect of foliar sprays with the following treatments on the downy mildew (Peronospora destructor and bulb rot (Burkholderia cepacia in onions: non-treated control, fungicide chlorotalonil/metalaxyl + chlorotalonil, potassium phosphite, foliar fertilizer (03-00-16, N-P-K, bordeaux mixture, bordeaux mixture/potassium phosphite, acibenzolar-S-methyl weekly applied; extract of alga Ulva fasciata and ulvan sprayed every 7, 14 and 21 days. Only the weekly spraying of fungicides and fertilizer (03-00-16, 400 mL/100 L significantly reduced the mildew severity by 60 and 23%, respectively, but did not increase the bulb yield. The foliar application of potassium rich fertilizers resulted in a higher incidence of rotten bulbs after 5 months in storage. Soluble sugar content and rot incidence of onion bulbs were significantly correlated (-0,629, p < 0,05.

João Américo Wordell Filho

2007-12-01

302

Atividade antioxidante in vitro de extratos de algumas algas verdes (Chlorophyta) do litoral catarinense (Brasil) / Antioxidant in vitro activity of extracts of some green seaweed (Chlorophyta) from southern Brazilian coast  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O efeito antioxidante de quatro espécies de algas marinhas do filo Chlorophyta (Codium decorticatum, Enteromorpha intestinalis, Ulva fasciata e Chaetomorpha anteninna) foi avaliado através da inibição da peroxidação do ácido linoléico em emulsão. Os extratos etéreos e metanólicos, na concentração de [...] 0,01%, foram obtidos por extração seqüencial das biomassas secas. As espécies mais efetivas sobre a peroxidação lipídica foram Enteromorpha intestinalis e Chaetomorpha anteninna, com porcentagens de inibição acima de 70%. A habilidade dos extratos metanólicos para seqüestrar o peróxido de hidrogênio foi avaliada, obtendo-se valores médios para porcentagens de captura entre 1,26% e 20,01%. Além disto, quantificaram-se os teores de clorofila a, carotenóides totais e compostos fenólicos totais nas biomassas algais. Os resultados indicam que as algas verdes estudadas são uma fonte promissora de compostos biologicamente ativos com propriedades antioxidantes. Abstract in english The antioxidant activity of four species of green seaweeds of the phylo Chlorophyta (Codium decorticatum, Enteromorpha intestinalis, Ulva fasciata and Chaetomorpha anteninna) collected at the seacoast of the State of Santa Catarina, Brazil, was evaluated by means of the inhibition of peroxidation of [...] linoleic acid converted into emulsion. Both ethereal and methanolic extracts were obtained from dried biomasses by sequential extraction procedures in concentrations of 0.01%. The most efficient species towards lipid peroxidation were E. intestinalis and C. anteninna with inhibition yields above 70% .The capacity of methanolic extracts to quench hydrogen peroxide was also estimated. Mean values varied from 1.26 to 20.01%. Chlorophylls a, total carotenoids and phenolic compounds were also quantified in the biomasses. Results indicated that studied green seaweeds are a very promising source of biologically active compounds with antioxidant properties.

Melissa dos Santos, Raymundo; Paulo, Horta; Roseane, Fett.

2004-12-01

303

Antioxidant and anticancer activities of selected persian gulf algae.  

Science.gov (United States)

In the present study, the effect of red (Gracillaria corticata), green (Ulva fasciata) and brown (Sargassum ilicifolium) seaweeds alcoholic extract, against five important human cancer cell lines (MCF-7, MDA-MB-231, HeLa, HepG2, and HT-29) proliferation, apoptosis and cell cycle arrest were evaluated. The reducing activity and total polyphenol content were also investigated. MTT assay was used for cytotoxicity test. Morphological alterations were examined using phase contrast, fluorescent and electron microscopy. All the extracts were antiproliferative against all the cancer cell lines, dose-dependently, with G. corticata methanol extract (GCME) having the greatest inhibition activity against MCF-7 cell line. The percentage of apoptosis increased from 18 to 78 %. The cell cycle analysis also showed that GCME can induce apoptosis which confirm by TEM. Algal extract reducing activities were as follows: G. corticata > S. ilicifolium > U. fasciata. The GCME is a good source of potential complementary and alternative functional food for prevention and treatment of cancer. PMID:24478544

Namvar, F; Baharara, J; Mahdi, A A

2014-01-01

304

Bioaccumulation of aluminium in the seagrasses Cymodocea nodosa (Ucria) Aschers. and Posidonia oceanica (L.) Delile and macroalgae of the Gulf of Antikyra (Greece)  

Energy Technology Data Exchange (ETDEWEB)

Mean concentrations of Aluminium ({+-}SE) in two seagrasses, four Chlorophytes, three Chromophytes and seven Rhodophyte species from the Gulf of Antikyra (Greece) decreased in the order: Chlorophyta > Chromophyta > Rhodophyta > P. oceanica > C. nodosa. This area is of particular interest because of the bauxite composition of the substrate and of the wate discharge from an aluminium factory in the Gulf. Aluminium concentrations in the two seagrasses were evenly distributed at the Gulf stations. Only the concentrations of Al in C. nodosa displayed significant seasonal variation. The mean concentrations increased from spring to summer, at which point they reached their maxima. This pattern is discussed in relation to the leaf-age of the seagrass. Regarding the amount of Al accumulated by the macroalgae of each division, there were interspecific differences which were attributed to differences in the structure, the ecology and the binding sites offered by the plants. The dependence of Al concentrations in the macrophytes on the concentrations in the sediment and the correlation of Al concentrations with the concentrations of some other metals in the plants were also investigated. (orig.)

Malea, P. [Thessaloniki Univ. (Greece). Inst. of Botany

1993-12-31

305

Assessment of Posidonia Oceanica (L. Delile conservation status by standard and putative approaches: the case study of Santa Marinella meadow (Italy, W Mediterranean  

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Full Text Available The conservation status of the Posidonia oceanica meadow at Santa Marinella (Rome was evaluated through both standard (bed density, leaf biometry, "A" coefficient, Leaf Area Index, rhizome production and biochemical/genetic approaches (total phenol content and Random Amplified Polymorphic DNA marker. The bio-chemical/genetic results are in agreement with those obtained by standard approaches. The bed under study was ranked as a disturbed one, due to its low density, and high heterogeneity in leaf biometry, LAI values, "A" coefficient and primary production. This low quality ranking is confirmed by both mean phenol content in plants, quite high and scattered, and by the low genetic variability in the meadow, with a very high similarity of specimen at a local scale. Hence, these two putative approaches clearly identify the endangered conservation status of the meadow. They link plant biodiversity and ecophysiology to ecosystem 'health'. Furthermore, they are repeatable and standardizable and could be usefully introduced in meadows monitoring to check environmental quality.

Alice Rotini

2011-08-01

306

Benthic Community zonation in a breakwater on the north coast of the state of Rio de Janeiro, Brazil  

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Full Text Available The present study presents the vertical zonation pattern of the intertidal benthic organisms on granitic boulders in the Farol de São Tomé beach (22º02'S, 41º03'W, Rio de Janeiro State. Four sampling profiles using 20 x 20 cm quadrats through a photo quadrat technique revealed four horizontal bands: (1 Littoral Fringe characterized by empty space and Littorina; (2 Upper eulittoral mainly occupied by Chthamalus and bare rock; (3 Lower eulittoral characterized by Chthamalus, Phragmatopoma, Ulva and Centroceras; and (4 Sublittoral Fringe characterized by Ulva, Phragmatopoma and Hypnea. The high variability in the relative abundance of the dominant species among the profiles may be attributed to the high topographic irregularity, reflecting in different wave action intensity at the same height, and consequently favoring the establishment of different species. The frequent substrate alternation from granitic rock to Phragmatopoma sandy reefs might contribute to the small-scale spatial variability. The implantation of a breakwater on an area without natural rocky shores allowed the study of intertidal benthic communty zonation, and provide a baseline for the experimental studies.O presente estudo teve como objetivo apresentar um esquema de zonação vertical de organismos bênticos da região entremarés em blocos graníticos na praia do Farol de São Tomé (22º02'S, 41º03'W na costa norte do estado do Rio de Janeiro. Quadrados de 20 x 20 cm foram sobrepostos em quatro perfis e utilizado o método por foto quadrat com 100 pontos de interseção. A análise de agrupamento e a porcentagem de cobertura de organismos bênticos indicadores sugeriram um padrão na distribuição vertical da região entremarés com quatro faixas: Franja do Supralittoral caracterizado por espaço vazio e Littorina ziczac; Médiolitoral Superior ocupado por Chthamalus spp e espaço vazio; médiolitoral inferior caracterizado por Chthamalus spp, Phragmatopoma lapidosa, Ulva fasciata e Centroceras clavulatum; e Franja do Infralitoral caracterizado por U. fasciata, P. lapidosa e Hypnea musciformis. A alta variabilidade na abundância relativa das espécies dominantes entre os perfis na zona Médiolitoral é atribuída a alta irregularidade topográfica do substrato, refletindo em diferentes intensidades de exposição às ondas em uma mesma altura, o que favorece o estabelecimento de diferentes espécies. A alternância freqüente do tipo de substrato de rocha granítica para recifes arenosos de P. lapidosa pode ter contribuído para a variabilidade espacial em pequena escala. Este estudo preenche uma lacuna referente à comunidade bêntica e sua distribuição vertical no norte do estado do Rio de Janeiro. A adição do molhe em uma área caracterizada pela ausência de substrato consolidado natural possibilitou o estudo da zonação da comunidade bêntica entremarés, oferecendo uma base de dados para futuros trabalhos experimentais.

Bruno Pereira Masi

2009-06-01

307

Bioprospecção de macroalgas marinhas e plantas aquáticas para o controle da antracnose do feijoeiro / Bioprospecting of marine seaweeds and aquatic plants for controlling the bean anthracnose  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O objetivo deste trabalho foi testar o efeito local, residual e sistêmico, de extratos de 17 espécies de macroalgas marinhas e de duas plantas aquáticas, sobre a antracnose do feijoeiro. Para tanto, os espécimes foram coletados, identificados, secos em estufa (50ºC/ 48 h), moídos e seus compostos ex [...] traídos com etanol. Plantas de feijoeiro (Phaseolus vulgaris cv. Uirapuru) foram cultivadas em vasos, em casa-de-vegetação. Os 19 extratos foram subdivididos e testados em duas etapas de seleção e comparação independentes, utilizando-se o delineamento inteiramente ao acaso, com cinco repetições (vasos com três plantas). As plantas foram pulverizadas com extratos na concentração de 50 mg de peso seco/mL quando apresentavam o primeiro trifólio expandido. Para verificar o efeito local, as plantas foram inoculadas com uma suspensão de 1,2 x 10(6) conídios/mL 4 horas após o tratamento, enquanto que para o estudo do efeito residual e sistêmico, as plantas foram inoculadas 7 dias após o tratamento. A severidade da antracnose foi avaliada 7 dias após a inoculação (dai) na planta inteira e no trifólio não tratado (efeito sistêmico), utilizando-se uma escala de 1 a 9. As algas e plantas que reduziram significativamente a severidade da doença foram comparadas em experimento avaliado aos 7 e aos 12 dai. O extrato de Bryothamnion seaforthii apresentou efeito local, reduzindo em 35% a severidade da antracnose, enquanto o extrato de Ulva fasciata demonstrou efeito residual com redução de 22% na doença aos 12 dai. Somente os extratos de Lemna sp. e U. fasciata reduziram sistemicamente a severidade de doença aos 7 dai na ordem de 55 e 44%, respectivamente, em relação à testemunha. O possível modo de ação desses extratos é discutido. Abstract in english The goal of this work was to test the local, residual as well as systemic effect of extracts from 17 marine seaweeds and two aquatic plant species against the bean anthracnose. For that, specimens were collected, identified, dried into an oven at 50ºC for 48 h, ground to powder and their compounds e [...] xtracted with ethanol. Bean plants (Phaseolus vulgaris cv. Uirapuru) were grown in pots under greenhouse conditions. The 19 extracts were shared into two independent groups for screening and comparison in a completely randomized design, with five replications (pot with 3 plants). Plants at the first trifoliolate leaf stage were sprayed with extracts at concentration of 50 mg dry weight/mL. To assess the local effect, plants were inoculated with a suspension of 1.2 x 10(6) conidia/mL 4 h after the treatment, whereas to study the residual and systemic effects, inoculation was performed after 7 days. Disease severity was evaluated 7 days after inoculation (dai) on either whole plants or the non-treated leaf (systemic effect), using a scale from 1 to 9. Seaweeds and plants which significantly reduced anthracnose were compared in a follow-up experiment evaluated at both 7 and 12 dai. The extract of Bryothamnion seaforthii revealed local effect reducing the anthracnose severity by 35%. Ulva fasciata extract showed residual effect reducing the anthracnose at 12 dai by 22%. Compared to control, only extracts of Lemna sp. and U. fasciata systemically reduced the disease severity at 7 dai by 55 e 44%, respectively. The possible mode of action of these extracts is discussed.

Guilherme Fernandes de, Abreu; Viviane, Talamini; Marciel João, Stadnik.

2008-02-01

308

Bioprospecção de macroalgas marinhas e plantas aquáticas para o controle da antracnose do feijoeiro Bioprospecting of marine seaweeds and aquatic plants for controlling the bean anthracnose  

Directory of Open Access Journals (Sweden)

Full Text Available O objetivo deste trabalho foi testar o efeito local, residual e sistêmico, de extratos de 17 espécies de macroalgas marinhas e de duas plantas aquáticas, sobre a antracnose do feijoeiro. Para tanto, os espécimes foram coletados, identificados, secos em estufa (50ºC/ 48 h, moídos e seus compostos extraídos com etanol. Plantas de feijoeiro (Phaseolus vulgaris cv. Uirapuru foram cultivadas em vasos, em casa-de-vegetação. Os 19 extratos foram subdivididos e testados em duas etapas de seleção e comparação independentes, utilizando-se o delineamento inteiramente ao acaso, com cinco repetições (vasos com três plantas. As plantas foram pulverizadas com extratos na concentração de 50 mg de peso seco/mL quando apresentavam o primeiro trifólio expandido. Para verificar o efeito local, as plantas foram inoculadas com uma suspensão de 1,2 x 10(6 conídios/mL 4 horas após o tratamento, enquanto que para o estudo do efeito residual e sistêmico, as plantas foram inoculadas 7 dias após o tratamento. A severidade da antracnose foi avaliada 7 dias após a inoculação (dai na planta inteira e no trifólio não tratado (efeito sistêmico, utilizando-se uma escala de 1 a 9. As algas e plantas que reduziram significativamente a severidade da doença foram comparadas em experimento avaliado aos 7 e aos 12 dai. O extrato de Bryothamnion seaforthii apresentou efeito local, reduzindo em 35% a severidade da antracnose, enquanto o extrato de Ulva fasciata demonstrou efeito residual com redução de 22% na doença aos 12 dai. Somente os extratos de Lemna sp. e U. fasciata reduziram sistemicamente a severidade de doença aos 7 dai na ordem de 55 e 44%, respectivamente, em relação à testemunha. O possível modo de ação desses extratos é discutido.The goal of this work was to test the local, residual as well as systemic effect of extracts from 17 marine seaweeds and two aquatic plant species against the bean anthracnose. For that, specimens were collected, identified, dried into an oven at 50ºC for 48 h, ground to powder and their compounds extracted with ethanol. Bean plants (Phaseolus vulgaris cv. Uirapuru were grown in pots under greenhouse conditions. The 19 extracts were shared into two independent groups for screening and comparison in a completely randomized design, with five replications (pot with 3 plants. Plants at the first trifoliolate leaf stage were sprayed with extracts at concentration of 50 mg dry weight/mL. To assess the local effect, plants were inoculated with a suspension of 1.2 x 10(6 conidia/mL 4 h after the treatment, whereas to study the residual and systemic effects, inoculation was performed after 7 days. Disease severity was evaluated 7 days after inoculation (dai on either whole plants or the non-treated leaf (systemic effect, using a scale from 1 to 9. Seaweeds and plants which significantly reduced anthracnose were compared in a follow-up experiment evaluated at both 7 and 12 dai. The extract of Bryothamnion seaforthii revealed local effect reducing the anthracnose severity by 35%. Ulva fasciata extract showed residual effect reducing the anthracnose at 12 dai by 22%. Compared to control, only extracts of Lemna sp. and U. fasciata systemically reduced the disease severity at 7 dai by 55 e 44%, respectively. The possible mode of action of these extracts is discussed.

Guilherme Fernandes de Abreu

2008-02-01

309

Temporal Variations of the Chemical Composition of Three Seaweeds in Two Tropical Coastal Environments  

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Full Text Available The seaweeds Chaetomorpha antennina, Gymnogongrus griffithsiae and Ulva fasciata were studied regarding tissue concentrations of total nitrogen, total phosphorus, total protein, hydrosoluble protein, total carbohydrate, chlorophyll a and total carotenoid throughout a 39-month survey in two coastal environments of Rio de Janeiro State, Brazil. One of the sites (Itapuca Stone has high concentrations of dissolved nutrients and an intense long-term process of cultural eutrophication; the second site (Bananal Inlet is thought to have lower concentrations of dissolved nutrients and no relevant anthropic impact. Seaweeds experienced changes in the concentrations of the substances in the thalli; however they did not show any cyclic seasonal pattern, except for pigments, with lower values in summer in both sites. The differences found for each species in each sampling at the sites were small (e.g. U. fasciata, more total nitrogen at Itapuca Stone or absent (e.g. C. antennina, no significant differences for hydrosoluble protein in the sites. Differences in the concentrations of dissolved nutrients in the sites did not generate contrasting chemical profiles in the seaweeds. There is no evidence of nitrogen- or phosphorus-limitation in any season. It is presumable that the concentrations of dissolved nutrients at the nutrient-poorer site are sufficient to generate high concentrations of the substances in the thalli of the species tested, similar to the concentrations measured in the eutrophic site. Experimental data are needed to elucidate the factors that promote the success of the species tested under contrasting nutrient availability and environmental disturbance.

Andyara Nascimento

2014-02-01

310

Are metals of antifouling paints transferred to marine biota?  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Devido sua alta toxicidade, o TBT está banido desde 2003, o que resultou na re-utilização de tintas a base de cobre. O objetivo deste trabalho é determinar se os metais provenientes das tintas anti-incrustantes (AFP) são transferidos para organismos bentônicos da Baía de Guanabara (BG) (Rio de janei [...] ro, Brasil). Concentrações de metais foram analisadas em duas espécies de algas Ulva flexuosa e U. fasciata e no isópoda, Sphaeroma serratum, em duas áreas de marinas em locais de substrato artificial coberto com tintas AFP e em locais de substrato natural. Também foram coletadas amostras em uma área oceânica (controle). Concentrações de Cd, Cr, Cu, Pb e Zn foram determinadas por Espectrofotometria de Absorção Atômica. Concentrações mais elevadas de Cu, Pb e Zn foram detectadas na BG em ambas espécies de algas em relação a área controle. Dentre as espécies de algas e do isópoda da BG, as populações coletadas sobre as superfícies cobertas com AFP apresentaram concentrações significativamente mais elevadas do que as populações do substrato natural. Os resultados obtidos demonstram que a liberação de metais presentes nas AFP dos decks e embarcações, estão sendo acumulados pelas algas e isópodas. Esses resultados indicam que o revestimento com AFP é a principal fonte de metais para a biota de marinas em áreas da BG. Abstract in english Because of its high toxicity, TBT (trybutiltin) was banned since 2003, which resulted in a greater re-use of Cu as based-biocide in antifouling paints (AFP). The aim of this work is to determine if metals form of AFP are transferred to benthic organisms from Guanabara Bay (GB) (Rio de Janeiro, Brazi [...] l). Metal concentrations were measured in two main fouling algae species Ulva flexuosa and U. fasciata and one isopod species, Sphaeroma serratum, in two GB marinas areas from sites with artificial substrate covered by AFP and natural substrate.In addition, control samples were collected in an adjacent open ocean area. Concentrations of Cd, Cr, Cu, Pb and Zn were determined by Atomic Absortion Spectrophotometry. Higher concentrations of Cu, Pb and Zn were detected in both algal species from GB in relation to control areas. Among samples of algae and isopod species from GB, populations collected over artificial surfaces covered by AFP presented significantly higher metal concentration than population of rocky natural substrate. Our data showed that the leaching of metals by antifouling paints present on decks and boats are being taken up by algae and isopods. These results indicate that antifouling coatings are the main source of heavy metal to biota of GB marina area.

Wladimir C., Paradas; Gilberto M., Amado Filho.

2007-03-01

311

Are metals of antifouling paints transferred to marine biota?  

Directory of Open Access Journals (Sweden)

Full Text Available Because of its high toxicity, TBT (trybutiltin was banned since 2003, which resulted in a greater re-use of Cu as based-biocide in antifouling paints (AFP. The aim of this work is to determine if metals form of AFP are transferred to benthic organisms from Guanabara Bay (GB (Rio de Janeiro, Brazil. Metal concentrations were measured in two main fouling algae species Ulva flexuosa and U. fasciata and one isopod species, Sphaeroma serratum, in two GB marinas areas from sites with artificial substrate covered by AFP and natural substrate.In addition, control samples were collected in an adjacent open ocean area. Concentrations of Cd, Cr, Cu, Pb and Zn were determined by Atomic Absortion Spectrophotometry. Higher concentrations of Cu, Pb and Zn were detected in both algal species from GB in relation to control areas. Among samples of algae and isopod species from GB, populations collected over artificial surfaces covered by AFP presented significantly higher metal concentration than population of rocky natural substrate. Our data showed that the leaching of metals by antifouling paints present on decks and boats are being taken up by algae and isopods. These results indicate that antifouling coatings are the main source of heavy metal to biota of GB marina area.Devido sua alta toxicidade, o TBT está banido desde 2003, o que resultou na re-utilização de tintas a base de cobre. O objetivo deste trabalho é determinar se os metais provenientes das tintas anti-incrustantes (AFP são transferidos para organismos bentônicos da Baía de Guanabara (BG (Rio de janeiro, Brasil. Concentrações de metais foram analisadas em duas espécies de algas Ulva flexuosa e U. fasciata e no isópoda, Sphaeroma serratum, em duas áreas de marinas em locais de substrato artificial coberto com tintas AFP e em locais de substrato natural. Também foram coletadas amostras em uma área oceânica (controle. Concentrações de Cd, Cr, Cu, Pb e Zn foram determinadas por Espectrofotometria de Absorção Atômica. Concentrações mais elevadas de Cu, Pb e Zn foram detectadas na BG em ambas espécies de algas em relação a área controle. Dentre as espécies de algas e do isópoda da BG, as populações coletadas sobre as superfícies cobertas com AFP apresentaram concentrações significativamente mais elevadas do que as populações do substrato natural. Os resultados obtidos demonstram que a liberação de metais presentes nas AFP dos decks e embarcações, estão sendo acumulados pelas algas e isópodas. Esses resultados indicam que o revestimento com AFP é a principal fonte de metais para a biota de marinas em áreas da BG.

Wladimir C. Paradas

2007-03-01

312

?-, ?-caroteno e ?-tocoferol em algas marinhas in natura ?- and ?-carotene, and ?-tocopherol in fresh seaweeds  

Directory of Open Access Journals (Sweden)

Full Text Available O objetivo deste trabalho foi avaliar o potencial de 32 espécies de algas marinhas das divisões Chlorophyta, Rhodophyta e Phaeophyta como fontes de ?- e ?-caroteno e ?-tocoferol. Todas as clorofíceas analisadas apresentaram ?- e ?-caroteno. Os teores máximo e mínimo de ?-caroteno foram detectados nas espécies do gênero Caulerpa e em Codium decorticatum, respectivamente; e ?-caroteno foi mais baixo em Caulerpa mexicana e mais elevado em Ulva fasciata. Dentre as rodofíceas, 11 espécies apresentaram ?-caroteno, com máximo em Botryocladia occidentalis. ?-caroteno foi encontrado em todas as algas vermelhas analisadas com teores mínimo e máximo em Gracilaria caudata e Bryothamnion triquetrum, respectivamente. As feofíceas apresentaram apenas ?-caroteno, com mínimo e máximo em Dictyopteris delicatula e Padina gymnospora, respectivamente. Na divisão Chlorophyta, ?-tocoferol, foi máximo em Codium decorticatum e mínimo em Caulerpa prolifera. Na Rhodophyta, 12 espécies apresentaram ?-tocoferol com teor máximo em Enantiocladia duperreyi. Na Phaeophyta, ?-tocoferol foi encontrado com valores mínimo e máximo em Lobophora variegata e Dictyota dichotoma, respectivamente.The aim of this work was to evaluate the potential of 32 marine macro algae species, members of Chlorophyta, Rhodophyta and Phaeophyta, as sources of a-carotene, b-carotene and a-tocopherol. Both b-carotene and a-carotene were found in all species of green macroalgae analyzed. The maximum content of a-carotene was detected in algae belonging to Caulerpa genus and the minimum in Codium decorticatum. The amount of b-carotene found was minimum in Caulerpa mexicana and maximum in Ulva fasciata. Among the Rhodophyta species, eleven contain a-carotene, the maximum content was found in Botryocladia occidentalis. b-Carotene was found in all red macroalgae analyzed presenting the lowest and highest values in Gracilaria caudata and Bryothamnion triquetrum, respectively. Species of Phaeophyta contained b-carotene but no a-carotene. The lowest value for b-carotene was found in Dictyopteris delicatula and the highest in Padina gymnospora. In Chlorophyta, the amount of a-tocopherol was maximum in Codium decorticatum and minimum in Caulerpa prolifera. In Rhodophyta, twelve species contained a-tocopherol, the highest value was found in Enantiocladia duperreyi. a-Tocopherol was detected in all Phaeophyta species analyzed. The highest and lowest values were found in Lobophora varigata and Dictyota dichotoma, respectively.

Márcia Barbosa de Sousa

2008-12-01

313

Comparative study of the estimated sample size for benthic intertidal species and communities Estudio comparativo del tamaño estimativo de muestra para especies bentónicas intermareales y de la comunidad  

Directory of Open Access Journals (Sweden)

Full Text Available The objective of this study was to determine the minimum sample size for studies of community structure and/or dominant species at different heights of a rocky intertidal zone at Rio de Janeiro. Community structure indicators suggested a variation in the minimum surface of 100 to 800 cm , with a minimum of 2 to 8 pro files and at least 20 to 80 quadrant sampling points, depending on the height. Indicators of species abundance suggest 100 cm for Hypnea musciformis and 400 cm for Ulva fasciata, Phragmatopoma lapidosa Kinberg, (1867 and Gymnogongrus griffthsiae at lower heights; 200 cm² for Chthamalus spp. at intermedíate heights; and 800 cm for Littorina ziczac at the greatest height. In general, seven to eight profiles and 10 to 20 sampling points were used. Different sample sizes were related to the abundance and spatial distributions of individual species, which varied at each intertidal height according to the degree of environmental stress.El objetivo de este estudio fue determinar el tamaño mínimo de muestra para estudios de estructura de la comunidad y para las especies dominantes a diferentes alturas, en una zona intermareal rocosa en Río de Janeiro. Los indicadores de la estructura de la comunidad sugirieron una variación en la superficie mínima de 100 a 800 cm , 2 a 8 el número mínimo de perfiles y 20 a 80 el número mínimo de puntos de muestreo de cuadrantes, dependiendo en la altura. Los indicadores de abundancia de especies sugieren 100 cm para Hypnea musciformis, 400 cm para Ulva fasciata, Phragmatopoma lapidosa Kinberg, (1867 y Gymnogongrus griffthsiae a las alturas inferiores; 200 cm² para Chthamalus spp. a las alturas mediales y 800 cm para Littorina ziczac a la altura superior. El número de perfiles y puntos de muestreo fue, en general, 7-8 y 10-20, respectivamente. Diferentes tamaños de la muestra fueron relacionados con la abundancia de especies individuales y su distribución espacial, que varían en cada altura de la zona intermareal de acuerdo con el grado de stress ambiental.

Danielle C Barbiero

2011-01-01

314

Comparative study of the estimated sample size for benthic intertidal species and communities / Estudio comparativo del tamaño estimativo de muestra para especies bentónicas intermareales y de la comunidad  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in spanish El objetivo de este estudio fue determinar el tamaño mínimo de muestra para estudios de estructura de la comunidad y para las especies dominantes a diferentes alturas, en una zona intermareal rocosa en Río de Janeiro. Los indicadores de la estructura de la comunidad sugirieron una variación en la su [...] perficie mínima de 100 a 800 cm , 2 a 8 el número mínimo de perfiles y 20 a 80 el número mínimo de puntos de muestreo de cuadrantes, dependiendo en la altura. Los indicadores de abundancia de especies sugieren 100 cm para Hypnea musciformis, 400 cm para Ulva fasciata, Phragmatopoma lapidosa Kinberg, (1867) y Gymnogongrus griffthsiae a las alturas inferiores; 200 cm² para Chthamalus spp. a las alturas mediales y 800 cm para Littorina ziczac a la altura superior. El número de perfiles y puntos de muestreo fue, en general, 7-8 y 10-20, respectivamente. Diferentes tamaños de la muestra fueron relacionados con la abundancia de especies individuales y su distribución espacial, que varían en cada altura de la zona intermareal de acuerdo con el grado de stress ambiental. Abstract in english The objective of this study was to determine the minimum sample size for studies of community structure and/or dominant species at different heights of a rocky intertidal zone at Rio de Janeiro. Community structure indicators suggested a variation in the minimum surface of 100 to 800 cm , with a min [...] imum of 2 to 8 pro files and at least 20 to 80 quadrant sampling points, depending on the height. Indicators of species abundance suggest 100 cm for Hypnea musciformis and 400 cm for Ulva fasciata, Phragmatopoma lapidosa Kinberg, (1867) and Gymnogongrus griffthsiae at lower heights; 200 cm² for Chthamalus spp. at intermedíate heights; and 800 cm for Littorina ziczac at the greatest height. In general, seven to eight profiles and 10 to 20 sampling points were used. Different sample sizes were related to the abundance and spatial distributions of individual species, which varied at each intertidal height according to the degree of environmental stress.

Danielle C, Barbiero; Isabela M, Macedo; Bruno, Mais; Ilana R, Zalmon.

315

Iron ore particles on four seaweed species from Camburi Beach (Espírito Santo state, Brazil  

Directory of Open Access Journals (Sweden)

Full Text Available The present study estimated the iron-ore concentration found on four species of seaweed. The species tested grow on a site heavily contaminated by this ore, in the city of Vitória, state of Espírito Santo, Brazil. Under natural conditions, the iron ore reached a temperature 5.0ºC higher than the sand on a sunny day. All the species had iron ore adhered to their fronds. Udotea cyathiformis was the species with the highest iron-ore concentration varing from 0.07 to 0.90 g wet weight, followed by Lobophora variegata (from 0.07 to 0.62 g wet weight, Padina gymnospora (from 0.08 to 0.55 g wet weight and Ulva fasciata (from 0.05 to 0.25 g wet weight. Even after four changes of water over a 12-hour period, the fronds still had particles adhered to their outside cell wall. All the species showed similar tendencies to release the iron, with the highest percentage of particles (40 to 60% released in the first change of water.Minério de ferro particulado sobre quatro macroalgas da Praia de Camburi (Estado do Espírito Santo-Brasil. O presente trabalho determinou a concentração de minério de ferro presente em quatro macroalgas. As espécies testadas ocorrem em um local extremamente contaminado por este particulado, na cidade de Vitória, Estado do Espírito Santo, Brasil. Sob condições naturais, o minério de ferro alcançou um temperatura de até 5,0ºC acima da temperatura da areia em um dia ensolarado.Todas as espécies estudadas apresentavam minério em suas paredes externas. A espécie Udotea cyathiformis apresentou a maior concentração de minério em sua fronde variando de 0,07 a 0,90 g massa úmida, seguida por Lobophora variegata (de 0,07 a 0,62 g massa úmida, Padina gymnospora (de 0,08 a 0,55 g massa úmida e Ulva fasciata (de 0,05 a 0,25 g massa úmida. Mesmo após sucessivas trocas de água, as frondes ainda apresentavam partículas aderidas às suas paredes celulares externas. As espécies apresentaram a mesma tendência de liberação de minério, sendo a maior percentagem do particulado liberada na primeira troca de água (40 a 60%.

Cristina Aparecida Gomes Nassar

2006-09-01

316

?-, ?-caroteno e ?-tocoferol em algas marinhas in natura / ?- and ?-carotene, and ?-tocopherol in fresh seaweeds  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O objetivo deste trabalho foi avaliar o potencial de 32 espécies de algas marinhas das divisões Chlorophyta, Rhodophyta e Phaeophyta como fontes de ?- e ?-caroteno e ?-tocoferol. Todas as clorofíceas analisadas apresentaram ?- e ?-caroteno. Os teores máximo e mínimo de ?-caroteno foram detectados na [...] s espécies do gênero Caulerpa e em Codium decorticatum, respectivamente; e ?-caroteno foi mais baixo em Caulerpa mexicana e mais elevado em Ulva fasciata. Dentre as rodofíceas, 11 espécies apresentaram ?-caroteno, com máximo em Botryocladia occidentalis. ?-caroteno foi encontrado em todas as algas vermelhas analisadas com teores mínimo e máximo em Gracilaria caudata e Bryothamnion triquetrum, respectivamente. As feofíceas apresentaram apenas ?-caroteno, com mínimo e máximo em Dictyopteris delicatula e Padina gymnospora, respectivamente. Na divisão Chlorophyta, ?-tocoferol, foi máximo em Codium decorticatum e mínimo em Caulerpa prolifera. Na Rhodophyta, 12 espécies apresentaram ?-tocoferol com teor máximo em Enantiocladia duperreyi. Na Phaeophyta, ?-tocoferol foi encontrado com valores mínimo e máximo em Lobophora variegata e Dictyota dichotoma, respectivamente. Abstract in english The aim of this work was to evaluate the potential of 32 marine macro algae species, members of Chlorophyta, Rhodophyta and Phaeophyta, as sources of a-carotene, b-carotene and a-tocopherol. Both b-carotene and a-carotene were found in all species of green macroalgae analyzed. The maximum content of [...] a-carotene was detected in algae belonging to Caulerpa genus and the minimum in Codium decorticatum. The amount of b-carotene found was minimum in Caulerpa mexicana and maximum in Ulva fasciata. Among the Rhodophyta species, eleven contain a-carotene, the maximum content was found in Botryocladia occidentalis. b-Carotene was found in all red macroalgae analyzed presenting the lowest and highest values in Gracilaria caudata and Bryothamnion triquetrum, respectively. Species of Phaeophyta contained b-carotene but no a-carotene. The lowest value for b-carotene was found in Dictyopteris delicatula and the highest in Padina gymnospora. In Chlorophyta, the amount of a-tocopherol was maximum in Codium decorticatum and minimum in Caulerpa prolifera. In Rhodophyta, twelve species contained a-tocopherol, the highest value was found in Enantiocladia duperreyi. a-Tocopherol was detected in all Phaeophyta species analyzed. The highest and lowest values were found in Lobophora varigata and Dictyota dichotoma, respectively.

Márcia Barbosa de, Sousa; Kelma Maria dos Santos, Pires; Daniel Barroso de, Alencar; Alexandre Holanda, Sampaio; Silvana, Saker-Sampaio.

2008-12-01

317

Iron ore particles on four seaweed species from Camburi Beach (Espírito Santo state, Brazil)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Minério de ferro particulado sobre quatro macroalgas da Praia de Camburi (Estado do Espírito Santo-Brasil). O presente trabalho determinou a concentração de minério de ferro presente em quatro macroalgas. As espécies testadas ocorrem em um local extremamente contaminado por este particulado, na cida [...] de de Vitória, Estado do Espírito Santo, Brasil. Sob condições naturais, o minério de ferro alcançou um temperatura de até 5,0ºC acima da temperatura da areia em um dia ensolarado.Todas as espécies estudadas apresentavam minério em suas paredes externas. A espécie Udotea cyathiformis apresentou a maior concentração de minério em sua fronde variando de 0,07 a 0,90 g massa úmida, seguida por Lobophora variegata (de 0,07 a 0,62 g massa úmida), Padina gymnospora (de 0,08 a 0,55 g massa úmida) e Ulva fasciata (de 0,05 a 0,25 g massa úmida). Mesmo após sucessivas trocas de água, as frondes ainda apresentavam partículas aderidas às suas paredes celulares externas. As espécies apresentaram a mesma tendência de liberação de minério, sendo a maior percentagem do particulado liberada na primeira troca de água (40 a 60%). Abstract in english The present study estimated the iron-ore concentration found on four species of seaweed. The species tested grow on a site heavily contaminated by this ore, in the city of Vitória, state of Espírito Santo, Brazil. Under natural conditions, the iron ore reached a temperature 5.0ºC higher than the san [...] d on a sunny day. All the species had iron ore adhered to their fronds. Udotea cyathiformis was the species with the highest iron-ore concentration varing from 0.07 to 0.90 g wet weight, followed by Lobophora variegata (from 0.07 to 0.62 g wet weight), Padina gymnospora (from 0.08 to 0.55 g wet weight) and Ulva fasciata (from 0.05 to 0.25 g wet weight). Even after four changes of water over a 12-hour period, the fronds still had particles adhered to their outside cell wall. All the species showed similar tendencies to release the iron, with the highest percentage of particles (40 to 60%) released in the first change of water.

Cristina Aparecida Gomes, Nassar; Yocie, Yoneshigue-Valentin.

2006-09-01

318

Environmental and behavioural factors affecting activity in the intertidal gastropod Hydrobia ulvae  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Laboratory microcosms were used to investigate the mud snail Hydrobia ulvac (Pennant) bioturbation activities and behavioural changes in response to snail density, algal food, sediment moisture content, light regime and water cover conditions. Density-dependent kinetics of bioturbated muddy areas were described by von Bertalanffy equations, which provided reliable estimates of mud surface covering rates by snail tracks (m(2) h(-1) snail(-1)), Snails need a wet habitat to be active either cove...

Orvain, Francis; Sauriau, Pierre-guy

2002-01-01

319

In vitro antioxidant activity of acetylated and benzoylated derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta).  

Science.gov (United States)

The antioxidant activity of natural ulvan and its derivatives (acetylated and benzoylated ulvans) in vitro was determined, including scavenging activity against superoxide and hydroxyl radicals, reducing power, and chelating ability. Obvious differences in antioxidant activity between natural ulvan and its derivatives were observed, moreover, the antioxidant activity of acetylated and benzoylated ulvans was stronger than that of natural ulvan. PMID:16481163

Qi, Huimin; Zhang, Quanbin; Zhao, Tingting; Hu, Rugui; Zhang, Kun; Li, Zhien

2006-05-01

320

Synthesis and antihyperlipidemic activity of acetylated derivative of ulvan from Ulva pertusa.  

Science.gov (United States)

In this study, acetylated ulvan (AU) was prepared with acetic anhydride in N,N-dimethylacetamide, and the antihyperlipidemic activity of natural ulvan and its acetylated ulvan derivative (AU) in mice was determined. Obvious differences in antihyperlipidemic activity between natural ulvan and its derivative were observed, moreover, AU showed stronger antihyperlipidemic activity on triglyceride (TG) and low density lipoprotein cholesterol (LDL-C). PMID:22115715

Qi, Huimin; Liu, Xiaolei; Zhang, Jinbao; Duan, Yu; Wang, Xiaolan; Zhang, Quanbin

2012-01-01

 
 
 
 
321

Mathematical-statistical model for analysis of Ulva algal net photosynthesis in Venice lagoon  

International Nuclear Information System (INIS)

The algal net photosynthesis, an important factor for the characterization of water quality in Venice lagoon, has been studied experimentally providing a mathematical model, validated by using statistical methods. This model relates oxygen production with irradiance, according to a well known law in biological literature. Its observed an inverted proportion between algal oxygen production and temperature, thus seasonality

322

METALE CI??KIE W WODZIE ORAZ PLECHACH ZIELENICY ULVA (ULVOPHYCEAE, CHLOROPHYTA)  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Glony maj? bardzo szerokie zastosowanie i znaczenie w przyrodzie. Mog? by? wska?nikami zanieczyszczenia wody oraz ?rodowiska w jakim si? znajduj?. Stanowi? cz?sto najwi?ksze ?ród?o biomasy w wodach, szczególnie morskich. Pe?ni? bardzo wa?n? funkcj? w utrzymaniu równowagi ?rodowiska wodnego a ich obecno?? jest czynnikiem niezb?dnym do prawid?owego funkcjonowania ekosystemów oceanicznych, morskich i s?odkowodnych. Formy planktonowe glonów odgrywaj? tak?e du?e znac...

?e?ska, Bogus?awa; Ptaszkiewicz, ?ukasz; Messyasz, Beata; Rybak, Andrzej

2010-01-01

323

Application of destructive neutron activation analysis for determination of microelements in Zostera marina and Ulva lactuca  

International Nuclear Information System (INIS)

The content of microelements has been determined in two algal species from the Bulgarian Black Sea coast by neutron activation analysis. The disturbing effects of the main components 24Na, 42K and 32P were eliminated by radiochemical separation. The results obtained for Fe, Co, Cr, Cd, Se, Zn, Ce, Sc, Rb, Au, Sm, As, Cu, Mn, Sb, and Hg are presented in ppm

324

FRESHWATER ULVA (CHLOROPHYTA) AS A BIOACCUMULATOR OF HEAVY METALS POLLUTION IN NIELBA RIVER (WIELKOPOLSKA REGION)  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Human activities still increase and causes negative impact to environment and consequently lead to ecological degradation. Water pollution, whose source are pesticides and chemical fertilizers from catchment areas, in result enrich the aquatic environment in heavy metals. These water contamination is accumulated in tissues of living organisms. A high degree of accumulation of dissolved metals is noticeable in macroalgae. To the one of the most tolerant group to high levels of water contaminat...

Messyasz, Beata; Rybak, Andrzej; Pikosz, Marta

2012-01-01

325

Charakterystyka inkrustacji pokrywaj?cych plechy s?odkowodnej formy zielenicy Ulva (Ulvaceae, Chlorophyta)  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Powstawanie inkrustacji w?glanu wapnia na p?dach ro?lin wodnych zachodzi jako efekt przeprowadzania procesu fotosyntezy w wodach zasadowych [Dodson, 1974]. Powstawanie inkrustacji na p?dach ro?lin wodnych jest procesem zachodz?cym szczególnie intensywnie w wodach o wysokim pH. Zwapnienie jako proces osadzania si? soli wapnia jest obserwowany równie? na powierzchni glonów. Odk?adanie si? w?glanu wapnia mo?e zachodzi? w ró?nych miejscach komórki: na zewn?trz, wewn?trz jak i...

Messyasz, Beata; Rybak, Andrzej; ?e?ska, Bogus?awa; Pikosz, Marta

2010-01-01

326

The taxonomy of ulva linnaeus, 1753, and enteromorpha link, 1820, (chlorophyceae) in the Netherlands  

Digital Repository Infrastructure Vision for European Research (DRIVER)

De meeste natuurlijke Nederlandse zeekusten zijn ongeschikt voor de groei van zeewieren. Denk maar aan de kale Noordzeestranden, en de zandplaten in de Waddenzee en langs de kusten van Z.W.-Nederland. Dit komt omdat zeewieren aangewezen zijn op vaste substraten voor hun vasthechting; op zand en slik kunnen ze zich niet goed bevestigen. ... Zie: Samenvatting

Koeman, Reinoud Pieter Teunis

1985-01-01

327

Screening of marine algae of Oman gulf for bio sorption of Cobalt  

International Nuclear Information System (INIS)

Batch experiments were conducted to study the ability of marine algae collected from Oman Gulf, Iran, for sorption of cobalt from the metal nitrate solution. The bio sorption experiments were performed using native and chemically pretreated sun-dried biomass of marine algae. Our finding indicated that MgCl2 pretreated Cystoseria indica, Sargassum glaucescens and Padina australis had more sorption capacities, while the CaCl2 pretreated ones showed lower capacity (comparing with non-treated native biomass). Also, a fall in the cobalt uptake capacity of Nizimuddinia zanardini, Gracilaria corticata, G. arcuata, Botryocladia leptopoda, Scinaia carnosa, Hypnea valentiae, Ulva fasciata and Codium sp. took place after treatment with chemicals, including CaCl2 (0.1 M), MgCl2 (0.1M), CaCl2 (0.1 M)/HCL (pH2) and HCl(0.1 M). Bio sorption of cobalt was rapidly took place onto algal bio sorbents and most of the sorbed metal ion was bound in the first minutes of contact. Uptake of cobalt was pH-dependent and the most cobalt removal occurred at pH 4. In our screening investigations, brown algae (Dictyota indica, N. zanardini, P. australis, S.glaucescens, and C. indica) removed cobalt most efficiently from aqueous solution, respectively. The capability of marine algae for separation of 60Co removal was demonstrated

328

Antiviral activity of extracts from Brazilian seaweeds against herpes simplex virus  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in english Organic extracts of 36 species of marine algae (sixteen species of Rhodophyta, eight species of Ochrophyta and twelve species of Chlorophyta) from seven locations on the Brazilian coast were evaluated for their anti-HSV-1 and anti-HSV-2 activity resistant to Acyclovir (ACV). Activity tests in crude [...] extracts, followed by the identification of the major compounds present, were performed for all species. The chemical profiles of all crude extracts were obtained by ¹H-NMR and 13C-NMR spectroscopy. The percentage of extracts with antiviral activity was higher for HSV-1 (86.1%) than for HSV-2 (55.5%). The green algae Ulva fasciata and Codium decorticatum both showed the highest activity (99.9%) against HSV-1, with triacylglycerols and fatty acids as the major components. The red alga Laurencia dendroidea showed good activity against HSV-1 (97.5%) and the halogenated sesquiterpenes obtusol and (-)-elatol were identified as the major components in the extract. Against HSV-2, the green alga Penicillus capitatus (Chlorophyta) and Stypopodium zonale (Ochrophyta) were the most active (96.0 and 95.8%). Atomaric acid, a meroditerpene, was identified as the major secondary metabolite in the S. zonale extract. These results reinforce the role of seaweeds as important sources of compounds with the potential to enter into the pipeline for development of new drugs against herpes simplex.

Angélica Ribeiro, Soares; Marcela C. S., Robaina; Gabriella S., Mendes; Thalia S. L., Silva; Lísia M. S., Gestinari; Odinéia S., Pamplona; Yocie, Yoneshigue-Valentin; Carlos R, Kaiser; Maria Teresa Villela, Romanos.

2012-08-01

329

Benthic Community zonation in a breakwater on the north coast of the state of Rio de Janeiro, Brazil  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese O presente estudo teve como objetivo apresentar um esquema de zonação vertical de organismos bênticos da região entremarés em blocos graníticos na praia do Farol de São Tomé (22º02'S, 41º03'W) na costa norte do estado do Rio de Janeiro. Quadrados de 20 x 20 cm foram sobrepostos em quatro perfis e ut [...] ilizado o método por foto quadrat com 100 pontos de interseção. A análise de agrupamento e a porcentagem de cobertura de organismos bênticos indicadores sugeriram um padrão na distribuição vertical da região entremarés com quatro faixas: Franja do Supralittoral caracterizado por espaço vazio e Littorina ziczac; Médiolitoral Superior ocupado por Chthamalus spp e espaço vazio; médiolitoral inferior caracterizado por Chthamalus spp, Phragmatopoma lapidosa, Ulva fasciata e Centroceras clavulatum; e Franja do Infralitoral caracterizado por U. fasciata, P. lapidosa e Hypnea musciformis. A alta variabilidade na abundância relativa das espécies dominantes entre os perfis na zona Médiolitoral é atribuída a alta irregularidade topográfica do substrato, refletindo em diferentes intensidades de exposição às ondas em uma mesma altura, o que favorece o estabelecimento de diferentes espécies. A alternância freqüente do tipo de substrato de rocha granítica para recifes arenosos de P. lapidosa pode ter contribuído para a variabilidade espacial em pequena escala. Este estudo preenche uma lacuna referente à comunidade bêntica e sua distribuição vertical no norte do estado do Rio de Janeiro. A adição do molhe em uma área caracterizada pela ausência de substrato consolidado natural possibilitou o estudo da zonação da comunidade bêntica entremarés, oferecendo uma base de dados para futuros trabalhos experimentais. Abstract in english The present study presents the vertical zonation pattern of the intertidal benthic organisms on granitic boulders in the Farol de São Tomé beach (22º02'S, 41º03'W), Rio de Janeiro State. Four sampling profiles using 20 x 20 cm quadrats through a photo quadrat technique revealed four horizontal bands [...] : (1) Littoral Fringe characterized by empty space and Littorina; (2) Upper eulittoral mainly occupied by Chthamalus and bare rock; (3) Lower eulittoral characterized by Chthamalus, Phragmatopoma, Ulva and Centroceras; and (4) Sublittoral Fringe characterized by Ulva, Phragmatopoma and Hypnea. The high variability in the relative abundance of the dominant species among the profiles may be attributed to the high topographic irregularity, reflecting in different wave action intensity at the same height, and consequently favoring the establishment of different species. The frequent substrate alternation from granitic rock to Phragmatopoma sandy reefs might contribute to the small-scale spatial variability. The implantation of a breakwater on an area without natural rocky shores allowed the study of intertidal benthic communty zonation, and provide a baseline for the experimental studies.

Bruno Pereira, Masi; Isabela Maria, Macedo; Ilana Rosental, Zalmon.

2009-06-01

330

EVALUATION OF GLYCOLIPIDS OF SOME EGYPTIAN MARINE ALGAE AS A SOURCE OF BIOACTIVE SUBSTANCES  

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Full Text Available Glycolipids in five species of marine algae: two species of Rhodophyta (Laurencia popillose, Galaxoura cylindriea; and one species of Chlorophyta (Ulva fasciata, and two species of Phaeophyta (Dilophys fasciola, Taonia atomaria collected from Red and Mediterranean sea, respectively were extracted, purified on silica gel column chromatography and identified by liquid chromatography MS/MS. Total glycolipid contents (GL (as % of total lipid were found to be in ranges 10.9 to 28.7%. T. atomaria had the highest level (28.7% followed by L. popillose (22.5 %. GL groups were analyzed for their sugars and fatty acids composition, and the result showed that the highest carbohydrate content of GLs were found in U. fasciata (6.05% and L. popillose (5.8%, and characterized by high content of monosaccharide: mannouronic acid, galactose and rhamnose. Amongst of the glycolipids of algal species, the most predominate fatty acid identified by GC were palmatic (C16:0 19.20 - 65.89% of total fatty acid, ecosatrienoic (C20:3 7.52 - 54.41%. GL analyzed by LC/MS/MS, revealed the peak at m/z 956 corresponding to the molecular formula of C51H104O17 was the most abundant molecular ion among all GLs of algal species and its fragments peaks at m/z 617(C37H58O4 and m/z 337 (C21H58O3, were tentatively identified as digalactosyldiacylglycerol (DGDG. The in vitro anticancer, antimicrobial, and antiviral activities of algal glycolipids were evaluated. GL of all algae species showed a remarkable antiviral activity in dose dependent manner. GL from D. fasciola has shown the most potent effect against HSV1 (IC50 of 10 µg/ml, comparable to that of the current antiviral drug acyclovir (IC50 55 µg/ml. On the other hand, GL of all algal species possessed a moderate antimicrobial activity. GL of T. atomaria exhibited a high inhibition effects against all test microorganisms, with MIC value ranged from 60 to 80 µg/ml. Moreover, all algal GL exhibited remarkable anticancer activities against both breast (MCF7 and liver human (HepG2 cancer cells, with an IC50 values ranging from 0.47 to 2.89 µg/ml.

El Baroty Gamal S

2011-03-01

331

Mathematical-statistical model for analysis of Ulva algal net photosynthesis in Venice lagoon; Modello matematico-statistico per l`analisi della produttivita` primaria dell`alga Ulva nella laguna di Venezia  

Energy Technology Data Exchange (ETDEWEB)

The algal net photosynthesis, an important factor for the characterization of water quality in Venice lagoon, has been studied experimentally providing a mathematical model, validated by using statistical methods. This model relates oxygen production with irradiance, according to a well known law in biological literature. Its observed an inverted proportion between algal oxygen production and temperature, thus seasonality.

Izzo, G.; Rizzo, V. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dip. Ambiente; Bella, A.; Picci, M. [Rome Univ. La Sapienza (Italy). Dip. di Statistica e Probabilita` Applicata; Giordano, P. [Rome Univ. La Sapienza (Italy). Dip. di Biologia Vegetale

1996-08-01

332

Detection of Epigenetic Variations in the Protoplast-Derived Germlings of Ulva reticulata Using Methylation Sensitive Amplification Polymorphism (MSAP)  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Regeneration of protoplasts into de novo plants was reported for a large number of seaweed species. The regeneration of protoplasts into different morphotypes as a result of epigenetic variations was discussed for the first time in this study. The loci assessed for methylation modifications in normal filamentous thalli showed a frequency of 32.43% as unmethylated DNA, 24.32% as a hemimethylated, and 20.27% as a methylation of internal cytosine at both the strands. The corresponding methylatio...

Gupta, Vishal; Bijo, A. J.; Kumar, Manoj; Reddy, C. R. K.; Jha, Bhavanath

2012-01-01

333

Variations of morphology and photosynthetic performances of Ulva prolifera during the whole green tide blooming process in the Yellow Sea.  

Science.gov (United States)

Since 2007, the world's largest macroalgal blooms have occurred along the coastal area of the Yellow Sea for 6 consecutive years. In 2012, shipboard surveying and satellite remote sensing were used to monitor the whole blooming process. The blooms originated in Rudong sea area of the South Yellow Sea where bloom patches were of dark green and filamentous thalli were the dominant morphology. The scale of the blooms reached its peak size in Rizhao sea area of the North Yellow Sea, and decreased promptly and became insignificant in Qingdao coast where the blooms turned yellow, mostly with air sac blades. Meanwhile, vegetative cells of the green tide algae changed into cytocysts gradually from which germ cells were released as the blooms drifted northward. Additionally, chlorophyll contents and fluorescence activity of free-floating thalli in the North Yellow Sea were both significantly lower than that in the South Yellow Sea. Those studies presented here contributed to increasing our understanding about how the green tide declined gradually in the North Yellow Sea. PMID:24054465

Zhang, Jian Heng; Huo, Yuan Zi; Zhang, Zheng Long; Yu, Ke Feng; He, Qing; Zhang, Lin Hui; Yang, Li Li; Xu, Ren; He, Pei Min

2013-12-01

334

REMOVAL OF AMMONIA TOXCITY IN MARINE SEDIMENT TIES: A COMPARISON OF ULVA LACTUCA, ZEOLITE AND AREATION METHODS  

Science.gov (United States)

Ammonia is suspected of causing some of the toxicity observed in marine sediment toxicity tests because it is sometimes found at elevated concentrations in marine interstitial waters. In marine waters, ammonia exists as un-ionized ammonia (NH3) and ammonium (NH4+) which combine ...

335

Two-step process using immobilized Saccharomyces cerevisiae and Pichia stipitis for ethanol production from Ulva pertusa Kjellman hydrolysate.  

Science.gov (United States)

We established a two-step production process using immobilized S. cerevisiae and P. stipitis yeast to produce ethanol from seaweed (U. pertusa Kjellman) hydrolysate. The process was designed to completely consume both glucose and xylose. In particular, the yeasts were immobilized using DEAE-corncob and DEAE-cotton, respectively. The first step of the process included a continuous column reactor using immobilized S. cerevisiae, and the second step included a repeated-batch reactor using immobilized P. stipitis. It was verified that the glucose and xylose in 20 L of medium containing the U. pertusa Kjellman hydrolysate was converted completely to about 5.0 g/l ethanol through the two-step process, in which the overall ethanol yield from total reducing sugar was 0.37 and the volumetric ethanol productivity was 0.126 g/ l/h. The volumetric ethanol productivity of the two-step process was about 2.7 times greater than that when P. stipitis was used alone for ethanol production from U. pertusa Kjellman hydrolysate. In addition, the overall ethanol yield from glucose and xylose was superior to that when P. stipitis was used alone for ethanol production. This two-step process will not only contribute to the development of an integrated process for ethanol production from glucose and xylose-containing biomass hydrolysates, but could also be used as an alternative method for ethanol production. PMID:23851267

Lee, Sang-Eun; Kim, Yi-Ok; Choi, Woo Yong; Kang, Do-Hyung; Lee, Hyeon-Yong; Jung, Kyung-Hwan

2013-10-28

336

Novel Antibacterial Proteins from the Microbial Communities Associated with the Sponge Cymbastela concentrica and the Green Alga Ulva australis? †  

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The functional metagenomic screening of the microbial communities associated with a temperate marine sponge and a green alga identified three novel hydrolytic enzymes with antibacterial activities. The results suggest that uncultured alpha- and gammaproteobacteria contain new classes of proteins that may be a source of antibacterial agents.

Yung, Pui Yi; Burke, Catherine; Lewis, Matt; Kjelleberg, Staffan; Thomas, Torsten

2011-01-01

337

Controle da mancha acinzentada da cebola e seu impacto sobre a qualidade de mudas / Control of onion leaf blight (Botrytis squamosa) and their impact on the seedling quality  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O presente trabalho estudou o efeito da adubação química e orgânica, fungicidas, fertilizantes foliares e extratos vegetais sobre a severidade da mancha acinzentada causada por Botrytis squamosa e a qualidade de mudas de cebola. Para tanto, realizou-se um experimento na Epagri/Estação Experimental d [...] e Ituporanga, SC, no período de maio a julho de 2005. Em um delineamento experimental de blocos casualizados com quatro repetições, em esquema de parcela sub-dividida, testou-se os seguintes tratamentos (Fator A): a) testemunha; b) fungicida ciprodinil (75 g i.a./100 L); c) fosfito de potássio (00-30-20, 250 mL/100 L); d) fertilizante foliar (03-00-16, 400 mL/100 L); e) calda bordalesa (0,3%); f) calda bordalesa/fosfito de potássio; g) extrato da alga Ulva fasciata (0,2%, peso seco: volume); h) extrato de cavalinha Equisetum arwense (0,26%); i) extrato de Urtiga dióica (0,47%). Os canteiros foram conduzidos com adubação química ou orgânica (Fator B). A área foliar necrosada foi avaliada semanalmente e usada para calcular a área abaixo da curva de progresso da doença (AACPD). O tipo de fertilização não afetou o desenvolvimento da mancha acinzentada, porém a adubação química aumentou o comprimento e o diâmetro do pseudocaule das mudas. Os tratamentos ciprodinil, fosfito de potássio, fertilizante foliar (03-00-16), calda bordalesa e fosfito combinado com calda bordalesa, reduziram significativamente a doença, enquanto que os extratos não foram eficazes. Abstract in english This work studied the effect of mineral and organic fertilization, fungicides, foliar fertilizers and plant extracts on the severity of onion leaf blight caused by Botrytis squamosa and onseedling quality. Field experiment was carried out at Epagri's experimental station in Ituporanga-SC from May to [...] July 2005. In a split-plot design with four replicates the following treatments (factor A) were tested: a) control; b) fungicide ciprodinil (75 g a.i./100 L); c) potassium fosfite (00-30-20, 250 mL./100 L); d) foliar fertilizer (03-00-16, 400 mL/100 L); e) Bordeaux mixture (0.3%); f) Bordeaux mixture/potassium fosfite; g) extract of the alga Ulva fasciata (0.2%, dry weight: volume); h) field horsetail (Equisetum arwense) extract (0.26%) and i) Urtiga dioica extract (0.47%). Seedling beds were established under mineral or organic fertilization (factor B). Leaf necrosis area (%) was weekly evaluated and used to calculate the area under disease progress curve (AUDPC). Fertilizer type did not affect the development of leaf blight, but the mineral fertilizer increased both plant height and pseudostem diameter. While plant extracts were not effective, the application of fungicide, potassium fosfite, foliar fertilizer (03-00-16), Bordeaux mixture alone or combined with fosfite, significantly reduced leaf blight.

João Américo, Wordell Filho; Marciel J, Stadnik.

338

Structural characterization and Biological Activity of Sulfolipids from selected Marine Algae  

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Full Text Available The sulfolipid classes (SLs in the total lipids of five species of marine algae, two species of Rhodophyta (Laurencia popillose, Galaxoura cylindriea, one species of Chlorophyta (Ulva fasciata, and two species of Phaeophyta (Dilophys fasciola, Taonia atomaria were separated and purified on DEAE-cellulose column chromatography. The SLs component was identified by IR, gas chromatography MS/MS and liquid chromatography MS/MS. The level of SLs contents va ried from 1.25% (in L. papillose to 11.82% (in D. fasciola of the total lipid contents. However, no significant differences in sulfate content (0.13 – 0.21% were observed among all these algae species. All SLs were characterized by high contents of palmitic acid (C 16:0, which ranged from 30.91% in G. cylindriea to 63.11% in T. atomatia. The main constitutes of algal sulfolipids were identified as sulfoquinovosyl-di-acylglycerol and sulfoquinovosyl acylglycerol. The sulfolipids of different algal species exhibited remarkable antiviral activity against herps simplex virus type 1 (HSV-1 with an IC50 ranging from 18.75 to 70. 2 ?g mL–1. Moreover, algal sulfolipid inhibited the growth of the tumor cells of breast and liver human cancer cells with IC50 values ranging from 0.40 to 0.67 ?g mL–1 for human breast adenocarcinoma cells (MCF7.Se separaron diferentes clases sulfolípidos (SL a partir de los lípidos totales de cinco especies de algas marinas: una especie de Chlorophyta (Ulva fasciata, dos especies de Phaeophyta (Dilophys fasciola, Taonia atomaria y dos especies de Rhodophyta (Laurencia popillose, Galaxoura cylindriea que se purificaron mediante cromatografía en columna de DEAE-celulosa. Los components de SLs fueron identificados por IR, cromatografía de gases MS/MS y cromatografía líquida MS/ MS. Los contenidos de SL en relación al total de lípidos varió de 1,25% (en L. papilosa al 11,82% (en D. fasciola. Sin embargo, no hay diferencias significativas en el contenido de sulfato observado entre todas estas especies de algas (desde 0,13 hasta 0,21%. Todos los SL se caracterizaron por un alto contenido de ácido palmítico (C16:0, que osciló entre 30,91% en G. cylindriea a 63,11% en T. atomatia. Sulfoquinovosyl-di-acilglicerol y acilglicerol sulfoquinovosyl fueron identificados como los principales constituyentes de los sulfolípidos de estas algas. Los sulfolípidos de las diferentes especies de algas estudiadas mostraron una notable actividad antiviral contra el virus del herpes simple tipo 1 (VHS-1 con una IC50 que osciló entre 18,75 y 70. 2 g mL–1. Por otra parte, los sulfolípidos de estas algas inhibieron el crecimiento de células tumorales de mama y células de cáncer de hígado humano con valores de IC50 que van desde 0,40 hasta 0,67 g mL–1 para las células de adenocarcinoma de mama humano (MCF7.

El Baz, F. K.

2013-12-01

339

Controle da mancha acinzentada da cebola e seu impacto sobre a qualidade de mudas Control of onion leaf blight (Botrytis squamosa and their impact on the seedling quality  

Directory of Open Access Journals (Sweden)

Full Text Available O presente trabalho estudou o efeito da adubação química e orgânica, fungicidas, fertilizantes foliares e extratos vegetais sobre a severidade da mancha acinzentada causada por Botrytis squamosa e a qualidade de mudas de cebola. Para tanto, realizou-se um experimento na Epagri/Estação Experimental de Ituporanga, SC, no período de maio a julho de 2005. Em um delineamento experimental de blocos casualizados com quatro repetições, em esquema de parcela sub-dividida, testou-se os seguintes tratamentos (Fator A: a testemunha; b fungicida ciprodinil (75 g i.a./100 L; c fosfito de potássio (00-30-20, 250 mL/100 L; d fertilizante foliar (03-00-16, 400 mL/100 L; e calda bordalesa (0,3%; f calda bordalesa/fosfito de potássio; g extrato da alga Ulva fasciata (0,2%, peso seco: volume; h extrato de cavalinha Equisetum arwense (0,26%; i extrato de Urtiga dióica (0,47%. Os canteiros foram conduzidos com adubação química ou orgânica (Fator B. A área foliar necrosada foi avaliada semanalmente e usada para calcular a área abaixo da curva de progresso da doença (AACPD. O tipo de fertilização não afetou o desenvolvimento da mancha acinzentada, porém a adubação química aumentou o comprimento e o diâmetro do pseudocaule das mudas. Os tratamentos ciprodinil, fosfito de potássio, fertilizante foliar (03-00-16, calda bordalesa e fosfito combinado com calda bordalesa, reduziram significativamente a doença, enquanto que os extratos não foram eficazes.This work studied the effect of mineral and organic fertilization, fungicides, foliar fertilizers and plant extracts on the severity of onion leaf blight caused by Botrytis squamosa and onseedling quality. Field experiment was carried out at Epagri's experimental station in Ituporanga-SC from May to July 2005. In a split-plot design with four replicates the following treatments (factor A were tested: a control; b fungicide ciprodinil (75 g a.i./100 L; c potassium fosfite (00-30-20, 250 mL./100 L; d foliar fertilizer (03-00-16, 400 mL/100 L; e Bordeaux mixture (0.3%; f Bordeaux mixture/potassium fosfite; g extract of the alga Ulva fasciata (0.2%, dry weight: volume; h field horsetail (Equisetum arwense extract (0.26% and i Urtiga dioica extract (0.47%. Seedling beds were established under mineral or organic fertilization (factor B. Leaf necrosis area (% was weekly evaluated and used to calculate the area under disease progress curve (AUDPC. Fertilizer type did not affect the development of leaf blight, but the mineral fertilizer increased both plant height and pseudostem diameter. While plant extracts were not effective, the application of fungicide, potassium fosfite, foliar fertilizer (03-00-16, Bordeaux mixture alone or combined with fosfite, significantly reduced leaf blight.

João Américo Wordell Filho

2006-12-01

340

Efeito do extrato de alga e da argila silicatada na severidade da alternariose e na produtividade da cebolinha comum (Allium fistulosum L.) / Effect of seaweed extract and silicate clay on Alternaria leaf spot and on the yield of green onion (Allium fistulosum L.)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese A alternariose é uma das doenças mais importantes da cebolinha em cultivo orgânico. Assim, o objetivo deste trabalho foi avaliar o efeito de pulverizações semanais de argila silicatada (Rocksil®) e extrato de Ulva fasciata no controle da doença e produtividade da cebolinha neste sistema de cultivo. [...] Avaliou-se a severidade final e a área abaixo da curva de progresso da doença nas folhas centrais (Yf e AACPDf), bem como da planta inteira (Yp e AACPDp). Na colheita, determinou-se o comprimento e peso fresco das folhas. O experimento foi repetido em quatro ciclos da cultura. Os dados de severidade da doença e produtividade foram submetidos à análise de variância e teste de Duncan (P?0.05). Na maioria dos ciclos experimentais, diferenças significativas foram verificadas na sanidade e produtividade dos tratamentos em relação à testemunha (plantas tratadas com água). Ambos preparados propiciaram reduções médias de 41 a 62% na severidade final (Yf e Yp) e de 28 a 58% na área abaixo da curva de progresso da doença (AACPDf e AACPDp). Os tratamentos das plantas com argila silicatada e extrato de alga aumentaram o peso fresco das folhas em média de 46% e 32%, respectivamente. Apesar deste trabalho indicar que a argila silicatada e o extrato de algo têm potencial para o controle da alternariose da cebolinha, mais estudos são necessários para elucidar os níveis de controle desses tratamentos em diferentes condições edafoclimáticas. ?0.05). Na maioria dos ciclos experimentais, diferenças significativas foram verificadas na sanidade e produtividade dos tratamentos em relação à testemunha (plantas tratadas com água). Ambos preparados propiciaram reduções médias de 41 a 62% na severidade final (Yf e Yp) e de 28 a 58% na área abaixo da curva de progresso da doença (AACPDf e AACPDp). Os tratamentos das plantas com argila silicatada e extrato de alga aumentaram o peso fresco das folhas em média de 46% e 32%, respectivamente. Apesar deste trabalho indicar que a argila silicatada e o extrato de algo têm potencial para o controle da alternariose da cebolinha, mais estudos são necessários para elucidar os níveis de controle desses tratamentos em diferentes condições edafoclimáticas. Abstract in english Alternaria leaf spot is one of the most important diseases of green onion in organic farming. The objective of this study was to evaluate the effect of weekly sprays of silicate clay (Rocksil®) and extract of Ulva fasciata in disease control and yield of the green onion in organic farming. Final dis [...] ease severity and area under the disease progress curve were evaluated on central leaves (Yl and AUDPCl) as well as the whole plant (Yp and AACPDp). Length and fresh weight of leaves was determined at harvest. The experiment was repeated in four crop cycles. Data on disease severity and yield were subjected to analysis of variance and Duncan test (P

Isaac B., Araújo; Luiz A.M., Peruch; Marciel J., Stadnik.

2012-10-01

 
 
 
 
341

Substrate selection of the caprellid Caprella dilatata (Crustacea, Amphipoda) / Selección de sustrato del caprélido Caprella dilatata (Crustacea, Amphipoda)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: English Abstract in spanish La diversidad del espacio y la variabilidad de las condiciones ambientales que afectan la calidad del hábitat para los organismos son factores determinantes en la selección de un hábitat ideal. El presente estudio tuvo como objetivo determinar la selección del sustrato del anfípodo caprélido Caprell [...] a dilatata, en ambientes naturales al largo de la costa del sur de Brasil y en condiciones de laboratorio. Se contabilizaron 31 sustratos biológicos distintos que albergaban a invertebrados, la mayoría fueron sustratos algales. C. dilatata fue registrada en once de estos sustratos, y resultó ser especie dominante únicamente en algas finamente ramificadas, ascidias, briozoos y en las superficies de las boyas y cuerdas. Los experimentos de laboratorio para selección de sustrato por C. dilatata se realizaron utilizando dos y cuatro especies de algas que tenían contrastante ramificación; Gracilaria cervicornis, Pterocladia capillacea, Sargassum cymosum y Ulva fasciata. En el primer experimento, caprélidos fueron trasladados a tres acuarios que contenían combinaciones variadas de dos sustratos de algas con un total de nueve combinaciones. Para el segundo experimento (cuatro algas), todas las especies de algas se combinaron en cada acuario. Los anfípodos obtenidos para experimentos en laboratorio mostraron el color del dermatoesqueleto siempre muy similar al color de las algas. Hubo una fuerte preferencia de caprélidos por el sustrato original. A pesar de la morfología cosmopolita de C. dilatata para la selección del sustrato, los individuos se encuentraron principalmente en sustratos estructuralmente complejos, y demostraron la importancia del camuflaje en estos caprélidos. Abstract in english The diversity of space and the variability of environmental conditions that affect habitat quality for organisms, are determining factors in the selection of an ideal habitat. The present study aimed to understand the substrate selectivity of a caprellid amphipod, Caprella dilatata, in natural envir [...] onments along the southern Brazilian coast and in laboratory conditions. A total of 31 biological substrates sheltering invertebrates were collected across various localities. C. dilatata was recorded in 11 substrate samples, and was a dominant species only in finely branched and softly surfaced algal substrate, ascidian and bryozoan colonies, and the surfaces of buoys and ropes. Laboratory experiments for substrate selection by C. dilatata were performed under two conditions: two-algal substrates and four-algal substrates. The phytals of Gracilaria cervicornis, Pterocladia capillacea, Sargassum cymosum and Ulva fasciata were selected for the experiments due to their contrasting degrees of ramification. In the first experiment (two-algal substrates), caprellids were transferred to three aquaria containing varied combinations of two algal substrates with a total of nine combinations. For the second experiment (four-algal substrates), all species of algae were combined together in each aquarium. Amphipods obtained for laboratory experiments always showed an exoskeleton color very close to that of the original algal substrate. Caprellids showed a strong preference for the original algal substrate. Despite the cosmopolitan morphology of C. dilatata for selection of the substrate, the individuals were mostly found in structurally complex substrates, and the experiments show the importance of camouflage for these caprellids.

Mariana B, Lacerda; Setuko, Masunari.

2011-08-01

342

Controle da mancha acinzentada da cebola e seu impacto sobre a qualidade de mudas / Control of onion leaf blight (Botrytis squamosa) and their impact on the seedling quality  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: Portuguese Abstract in portuguese O presente trabalho estudou o efeito da adubação química e orgânica, fungicidas, fertilizantes foliares e extratos vegetais sobre a severidade da mancha acinzentada causada por Botrytis squamosa e a qualidade de mudas de cebola. Para tanto, realizou-se um experimento na Epagri/Estação Experimental d [...] e Ituporanga, SC, no período de maio a julho de 2005. Em um delineamento experimental de blocos casualizados com quatro repetições, em esquema de parcela sub-dividida, testou-se os seguintes tratamentos (Fator A): a) testemunha; b) fungicida ciprodinil (75 g i.a./100 L); c) fosfito de potássio (00-30-20, 250 mL/100 L); d) fertilizante foliar (03-00-16, 400 mL/100 L); e) calda bordalesa (0,3%); f) calda bordalesa/fosfito de potássio; g) extrato da alga Ulva fasciata (0,2%, peso seco: volume); h) extrato de cavalinha Equisetum arwense (0,26%); i) extrato de Urtiga dióica (0,47%). Os canteiros foram conduzidos com adubação química ou orgânica (Fator B). A área foliar necrosada foi avaliada semanalmente e usada para calcular a área abaixo da curva de progresso da doença (AACPD). O tipo de fertilização não afetou o desenvolvimento da mancha acinzentada, porém a adubação química aumentou o comprimento e o diâmetro do pseudocaule das mudas. Os tratamentos ciprodinil, fosfito de potássio, fertilizante foliar (03-00-16), calda bordalesa e fosfito combinado com calda bordalesa, reduziram significativamente a doença, enquanto que os extratos não foram eficazes. Abstract in english This work studied the effect of mineral and organic fertilization, fungicides, foliar fertilizers and plant extracts on the severity of onion leaf blight caused by Botrytis squamosa and onseedling quality. Field experiment was carried out at Epagri's experimental station in Ituporanga-SC from May to [...] July 2005. In a split-plot design with four replicates the following treatments (factor A) were tested: a) control; b) fungicide ciprodinil (75 g a.i./100 L); c) potassium fosfite (00-30-20, 250 mL./100 L); d) foliar fertilizer (03-00-16, 400 mL/100 L); e) Bordeaux mixture (0.3%); f) Bordeaux mixture/potassium fosfite; g) extract of the alga Ulva fasciata (0.2%, dry weight: volume); h) field horsetail (Equisetum arwense) extract (0.26%) and i) Urtiga dioica extract (0.47%). Seedling beds were established under mineral or organic fertilization (factor B). Leaf necrosis area (%) was weekly evaluated and used to calculate the area under disease progress curve (AUDPC). Fertilizer type did not affect the development of leaf blight, but the mineral fertilizer increased both plant height and pseudostem diameter. While plant extracts were not effective, the application of fungicide, potassium fosfite, foliar fertilizer (03-00-16), Bordeaux mixture alone or combined with fosfite, significantly reduced leaf blight.

João Américo, Wordell Filho; Marciel J, Stadnik.

2006-12-01

343

Tratamiento de efluentes del cultivo de Litopenaeus vannamei mediante procesos de sedimentación, filtración y absorción / Treatments of effluents from Litopenaeus vannamei shrimp cultures through sedimentation, filtration and absorption  

Scientific Electronic Library Online (English)

Full Text Available SciELO Chile | Language: Spanish Abstract in spanish [...] Abstract in english La eficiencia de remoción de materia particulada y nutrientes disueltos de efluentes de estanques de reproductores de Litopenaeus vannamei fue evaluada a escala de laboratorio, empleando tratamientos de sedimentación, filtración por Crassostrea rhizophorae y absorción por Ulva fasciata. En cada trat [...] amiento se empleó un tiempo de residencia hidráulica del efluente de seis horas. En la etapa de sedimentación se utilizaron estanques con 90 L del efluente. Para la etapa de filtración, se emplearon estanques con 20 L del efluente sobrenadante de la etapa de sedimentación. En la absorción se utilizaron estanques con 15 L del efluente sobrenadante de la etapa de filtración. Al final de cada etapa se colectaron muestras de 500 mL para los análisis de calidad de agua. Los resultados indicaron que en la sedimentación, la turbidez, clorofila-a y sólidos suspendidos totales presentaron una remoción de 93,8%, 94,5% y 65,9%, respectivamente. La filtración alcanzó eficiencias de 89,3% y 100% para bacterias totales y clorofila a, respectivamente. En la absorción, fosfato (PO4-3 ), amonio (N-NH4-) y nitrato (N-NO3) fueron reducidos en 53,6%, 49,6% y 70,2%, respectivamente, en relación a los valores del estanque control. Los tratamientos combinados de sedimentación, filtración y absorción, bacterias totales (95,1%), turbidez (97,1%) sólidos suspendidos totales (81,3%), clorofila-a (99,1%), amonio (54,1%), nitrito (58,0%), nitrato (69,2%) y fosfato (52,9%), presentaron una alta eficiencia de remoción, en relación a los valores del efluente bruto. Se concluye que la combinación de tratamientos mejora significativamente la calidad de los efluentes del cultivo del camarón Litopenaeus vannamei.

Roberto, Ramos; Luis, Vinatea; Julia, Santos; Rejane, Da Costa.

344

Tratamiento de efluentes del cultivo de Litopenaeus vannamei mediante procesos de sedimentación, filtración y absorción Treatments of effluents from Litopenaeus vannamei shrimp cultures through sedimentation, filtration and absorption  

Directory of Open Access Journals (Sweden)

Full Text Available La eficiencia de remoción de materia particulada y nutrientes disueltos de efluentes de estanques de reproductores de Litopenaeus vannamei fue evaluada a escala de laboratorio, empleando tratamientos de sedimentación, filtración por Crassostrea rhizophorae y absorción por Ulva fasciata. En cada tratamiento se empleó un tiempo de residencia hidráulica del efluente de seis horas. En la etapa de sedimentación se utilizaron estanques con 90 L del efluente. Para la etapa de filtración, se emplearon estanques con 20 L del efluente sobrenadante de la etapa de sedimentación. En la absorción se utilizaron estanques con 15 L del efluente sobrenadante de la etapa de filtración. Al final de cada etapa se colectaron muestras de 500 mL para los análisis de calidad de agua. Los resultados indicaron que en la sedimentación, la turbidez, clorofila-a y sólidos suspendidos totales presentaron una remoción de 93,8%, 94,5% y 65,9%, respectivamente. La filtración alcanzó eficiencias de 89,3% y 100% para bacterias totales y clorofila a, respectivamente. En la absorción, fosfato (PO4-3 , amonio (N-NH4- y nitrato (N-NO3 fueron reducidos en 53,6%, 49,6% y 70,2%, respectivamente, en relación a los valores del estanque control. Los tratamientos combinados de sedimentación, filtración y absorción, bacterias totales (95,1%, turbidez (97,1% sólidos suspendidos totales (81,3%, clorofila-a (99,1%, amonio (54,1%, nitrito (58,0%, nitrato (69,2% y fosfato (52,9%, presentaron una alta eficiencia de remoción, en relación a los valores del efluente bruto. Se concluye que la combinación de tratamientos mejora significativamente la calidad de los efluentes del cultivo del camarón Litopenaeus vannamei.

Roberto Ramos

2010-01-01

345

Charakterystyka morfometryczna i siedliskowa makrozielenicy Ulva prolifera O.F. Müller J. Agardh 1883 (Chlorophyta, Ulvophyceae) na s?odkowodnym stanowisku w Wielkopolsce  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Badania nad morfometri? i czynnikami fizyczno-chemicznymi kszta?tuj?cymi rozwój U. prolifera (Enteromorpha prolifera) prowadzono od maja do ko?ca lipca 2008 r. Stanowisko badawcze zlokalizowano na stawie przep?ywowym we wsi Tulce pod Poznaniem (Wielkopolska). Stwierdzone miejsce wyst?powania U. prolifera jest szóstym ?ródl?dowym stanowiskiem tego gatunku w Polsce. Do analiz morfometrycznych i struktury plechy pobrano szereg prób. Odnaleziony gatunek makrozielenicy cechowa? si? z...

Messyasz, Beata; Rybak, Andrzej

2009-01-01

346

Nutritional composition and physicochemical properties of two green seaweeds (Ulva pertusa and U. intestinalis) from the Pattani Bay in Southern Thailand  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The chemical composition, amino acid, and element contents, as well as some physicochemical properties of Ulvapertusa and U. intestinalis, collected from the Pattani Bay in Southern Thailand in the rainy and summer seasons of 2007–2008, were investigated in order to gain more nutritional information. It was found that the two green seaweed speciescontained high level of protein (14.6–19.5% DW), lipid (2.1–8.7% DW), ash (25.9–28.6% DW), soluble fiber (25.3–39.6% DW),insoluble fiber (...

Ommee Benjama; Payap Masniyom

2011-01-01

347

Nutritional composition and physicochemical properties of two green seaweeds (Ulva pertusa and U. intestinalis from the Pattani Bay in Southern Thailand  

Directory of Open Access Journals (Sweden)

Full Text Available The chemical composition, amino acid, and element contents, as well as some physicochemical properties of Ulvapertusa and U. intestinalis, collected from the Pattani Bay in Southern Thailand in the rainy and summer seasons of 2007–2008, were investigated in order to gain more nutritional information. It was found that the two green seaweed speciescontained high level of protein (14.6–19.5% DW, lipid (2.1–8.7% DW, ash (25.9–28.6% DW, soluble fiber (25.3–39.6% DW,insoluble fiber (21.8–33.5% DW and total dietary fiber (51.3–62.2% DW. Comparing the element contents of the two species,U. pertusa was rich in Mg, K and Ca, while U. intestinalis was rich in Mg, K, Cl, Na, and Ca. The essential amino acids of thetwo species were rich in leucine, valine, and arginine contents. The most limiting essential amino acid of both species waslysine. However, the nutritional composition of the two seaweeds varied depending on seasonal change. As for the physicochemicalproperties of both seaweeds, their swelling capacity (SWC, water holding capacity (WHC, and oil holding capacity(OHC ranged from 4.0 to 6.4 ml/g DW, 7.8 to 15.0 g/g DW and 1.4 to 4.8 g oil/g DW, respectively. WHC and OHC of U.intestinalis was higher than those of U. pertusa (P<0.05. This study suggested that both species could be potentially usedas raw materials or ingredients to improve the nutritive value and texture of functional food and healthy products for humanbeings.

Ommee Benjama

2011-10-01

348

THE EFFECT OF AN HERBIVOROUS ESTUARINE AMPHIPOD, ALLORCHESTES ANGUSTA, IN THE BIOMASS ACCUMULATION OF THE GREEN MACROALGAE, ENTEROMORPHA INTESTINALIS AND ULVA EXPANSA. (R827637)  

Science.gov (United States)

The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

349

Seasonal variability of community structure and breeding activity in marine phytal harpacticoid copepods on Ulva pertusa from Pohang, east coast of Korea  

Science.gov (United States)

Seasonal changes in community structure and reproductive status of phytal harpacticoid copepods in the shallow sublittoral bottom at two sites (Masan-ri and Guryongpo) in Pohang (Korea) are described monthly over a period of 1 year (October 1996 to September 1997). A total of 36 harpacticoid species was identified and the numerically dominant copepods were made up of the families Porcellidiidae and Tisbidae. Although the number of species did not show a seasonal trend, total harpacticoid density revealed a favorable distribution for the warmer season (spring and summer) at both sites. Multiple linear regression analyses showed that univariate indices such as density, evenness and diversity are closely associated with certain environmental parameters. For example, the dominant species fluctuated seasonally in abundance and their maximum densities were found to be temperature- (+ with Porcellidium ofunatense) and nutrient-dependent (+ with Scutellidium longicauda acheloides, + with Zaus unisetosus, and - with P. wandoensis). In addition, the dominant species appeared to breed year round and their reproductive indices are significantly correlated with some environmental parameters such as temperature (-), pH (+), and phosphate (+). Three species ( S. l. acheloides, P. wandoensis, and P. ofunatense) showed maximum density two or three months after their reproductive activity reached a maximum. Overall, the seasonal changes in a phytal harpacticoid community could be explained by combinations of environmental parameters supporting the complexity and biodiversity for this specific group of species in coastal ecosystems.

Song, Sung Joon; Ryu, Jongseong; Khim, Jong Seong; Kim, Won; Yun, Sung Gyu

2010-01-01

350

Enzymatic regulation of photosynthetic and light-independent carbon fixation in Laminaria setchellii (Phaeophyta), Ulva lactuca (Chlorophyta) and Iridaea cordata (Rhodophyta) Regulación enzimática de la fotosíntesis y la fijación de carbono en obscuridad por Laminaria setchellii (Phaeophyta), Ulva lactuca (Chlorophyta) e Iridaea cordata (Rhodophyta)  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Carbon is acquired through photosynthetic and non-photosynthetic processes in marine algae. However, little is known about the biochemical regulation of these metabolic pathways along the thallus of seaweeds. Consequently, the objective of this study was to assess the distribution of in vivo carboxylation pathways and to relate them to the in vitro activity of ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO), phosphoenolpyruvate carboxykinase (PEPCK), and phosphoenolpyruvate carboxyl...

ALEJANDRO CABELLO-PASINI; Alberte, Randall S.

2001-01-01

351

Diversity in the seeds of Trigonella occulta Defile  

Directory of Open Access Journals (Sweden)

Full Text Available Dimorphism in the seeds of Trigonella occulta Delile of 4 studied seed provenances has been observed. The seeds of various origin show also diversity in their germination and imbibition behaviour, however low temperature of nearly 10°C is essential for germination in all cases. There is the correlation between morphological and physiological characteristics of some seed lots.

Ramesh C. Bhatia

1983-03-01

352

AcEST: BP916672 [AcEST  

Full Text Available YMU001_000090_C04 508 Adiantum capillus-veneris mRNA. clone: YMU001_000090_C04. BP916672 CL27Con ... ... 48 2e-05 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 47 7e-05 sp|P13133|PLAS_ ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 45 2e-04 sp|P07465|PLAS_ ...

353

Antioxidant Properties of two Edible Green Seaweeds From Northern Coasts of the Persian Gulf  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Background: Ulva genus, an edible seaweed, and an important food source in many south-east Asian countries is also recognized by its synonymous name as Enteromorpha.Objectives: This study was carried out to evaluate antioxidant activity, contents of total phenolics, and flavonoids of methanolic extracts of edible green seaweeds including Ulva clathrata (Roth) C. Agardh and three samples of Ulva prolifera O.F.Müller grown at different parts of Bushehr Province along the northern coasts of the...

Massoumeh Farasat; Ramazan-Ali Khavari-Nejad; Seyed Mohammad Bagher Nabavi; Foroogh Namjooyan

2013-01-01

354

AcEST: DK955489 [AcEST  

Full Text Available TST39A01NGRL0023_D18 638 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0023_D18. 5' end seq ... ... 111 3e-24 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 110 6e-24 sp|P13133|PLAS ... _ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 10 ...

355

Sulfur isotope variability of oceanic DMSP generation and its contributions to marine biogenic sulfur emissions  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Oceanic dimethylsulfoniopropionate (DMSP) is the precursor to dimethylsulfide (DMS), which plays a role in climate regulation through transformation to methanesulfonic acid (MSA) and non-seasalt sulfate (NSS-SO42?) aerosols. Here, we report measurements of the abundance and sulfur isotope compositions of DMSP from one phytoplankton species (Prorocentrum minimum) and five intertidal macroalgal species (Ulva lactuca, Ulva linza, Ulvaria obscura, Ulva prolifera, and Polysiphonia hendryi) in ma...

Oduro, Harry; Alstyne, Kathryn L.; Farquhar, James

2012-01-01

356

AcEST: BP916965 [AcEST  

Full Text Available YMU001_000094_B02 350 Adiantum capillus-veneris mRNA. clone: YMU001_000094_B02. BP916965 CL27Con ... e... 43 6e-04 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 42 7e-04 sp|P13133|PLAS_ ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 41 0.002 sp|P07465|PLAS_ ...

357

Marine Bacteria from Danish Coastal Waters Show Antifouling Activity against the Marine Fouling Bacterium Pseudoalteromonas sp. Strain S91 and Zoospores of the Green Alga Ulva australis Independent of Bacteriocidal Activity?†  

Digital Repository Infrastructure Vision for European Research (DRIVER)

The aims of this study were to determine if marine bacteria from Danish coastal waters produce antifouling compounds and if antifouling bacteria could be ascribed to specific niches or seasons. We further assess if antibacterial effect is a good proxy for antifouling activity. We isolated 110 bacteria with anti-Vibrio activity from different sample types and locations during a 1-year sampling from Danish coastal waters. The strains were identified as Pseudoalteromonas, Phaeobacter, and Vibrio...

Bernbom, Nete; Ng, Yoke Yin; Kjelleberg, Staffan; Harder, Tilmann; Gram, Lone

2011-01-01

358

The underestimation of seagrass biological cycle in the biomonitoring of coastal trace element pollution  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Previous monitoring surveys using the Mediterranean seagrass Posidonia oceanica (L.) Delile mostly studied coastal pollutions in Cr, Ni, Cu, Zn, Cd, Pb and/or Fe and the distribution of their bioaccumulated levels between the seagrass compartments. In contrast, other trace elements (TEs) like As, V, Ag, Be, Al, Mn, Co, Se, Mo, Sn, Sb and Bi, many of them categorized as TEs of environmental emerging concern, have been subject to nearly no ecotoxicological survey with that species. Moreover, th...

Richir, Jonathan; Sartoretto, Ste?phane; Gobert, Sylvie

2013-01-01

359

Anti-Helicobacter pylori and Urease Inhibition Activities of Some Traditional Medicinal Plants  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Different parts of Acacia nilotica (L.) Delile, Calotropis procera (Aiton) W.T. Aiton, Adhatoda vasica Nees, Fagoniaar abica L. and Casuarina equisetifolia L. are traditionally used in folk medicine for the treatment of a variety of common ailments like nausea, cold, cough, asthma, fevers, diarrhea, sore throat, swelling, etc. The present study was aimed to evaluate the anti-Helicobacter pylori and urease inhibition activities of extracts produced from the above selected medicinal plants nati...

Tahir Mehmood; Nazamid Saari; Fauqia Naz; Farooq Anwar; Muhammad Amin

2013-01-01

360

A Summary of Reports of Ulvaceae (Chlorophyta From Turkey*  

Directory of Open Access Journals (Sweden)

Full Text Available A revised list of the taxa of the Ulvaceae occurring in Turkey is reported in this research. A total of 25 taxa of this family is herein reported from Turkey. Also, the transfers of four taxa of Enteromorpha to Ulva are proposed: Ulva intestinalis L. var. asexualis (Bliding E. Taskin comb. nov. (Enteromorpha intestinalis var. asexualis Bliding, 1963, Ulva intestinalis forma ramosa (Vinogradova E. Taskin comb. nov. (Enteromorpha intestinalis forma ramosa Vinogradova, 1974, Ulva intestinalis forma saprobia (Vinogradova E. Taskin comb. nov. (Enteromorpha intestinalis forma saprobia Vinogradova, 1974 and Ulva prolifera subsp. gullmariensis (Bliding E. Taskin comb. nov. (Enteromorpha prolifera subsp. gullmariensis Bliding, 1963. A total of 25 taxa (species and intraspecific reported from Turkey.

Ergun Taskin

2007-01-01

 
 
 
 
361

Regulation of spatial and seasonal variation of macroalgal biomass in a brackish, eutrophic lake  

Science.gov (United States)

Processes leading to biomass variation of Ulva were investigated at two contrasting sites in the eutrophic Veerse Meer (The Netherlands). Ulva species dominated at the Middelplaten site, while at the Kwistenburg site a mixture of Ulva spp. and Chaetomorpha linum dominated. Total summer macroalgal biomass was higher at Middelplaten than at Kwistenburg (282 and 79 g DW m-2, respectively). Growth rates of Ulva spp. were high at both sites in May 1992 (cage mean 0.28-0.30 day-1), but quickly dropped to lower values (0.05-0.10 day-1). In May, growth rates were significantly highest at Kwistenburg, while during the rest of the season growth rates were similar for both sites. Temperature, pH, dissolved oxygen, salinity, light attenuation, phytoplankton and nutrient concentrations did not differ between sites. The relation between variation in Ulva spp. growth rates and environmental parameters was analysed using stepwise multiple regression, showing that light and temperature were the main variables regulating Ulva spp. growth rates. As Ulva growth rates were similar for both sites but biomass was much lower at Kwistenburg it was concluded that a large amount of produced biomass was lost at Kwistenburg. Although the exact reason for this remains unclear, it seems most likely that transport of macroalgae by wind and waves is a very important factor. This study shows the importance of simultaneously measuring growth rates and biomass at a high temporal resolution to reveal the mechanisms responsible for spatial variation in macroalgal biomass in shallow coastal areas.

Malta, Erik-jan; Verschuure, Jacobus; Nienhuis, Pieter

2002-08-01

362

Raptors and "campo-cerrado" bird mixed flock led by Cypsnagra Hirundinacea (Emberizidae:Thraupinae) / Predadores aéreos e bandos mistos de aves, em campo-cerrado, liderados por Cypsnagra hirundinacea (Emberizidae: Thraupinae)  

Scientific Electronic Library Online (English)

Full Text Available SciELO Brazil | Language: English Abstract in portuguese Bandos mistos de aves incluindo Cypsnagra hirundinacea e Neothraupis fasciata como espécies que vigiam por sentinelas foram estudados em campo-cerrado de março a setembro de 1996. O propósito desse estudo foi verificar a possível relação entre o grau de alerta de uma espécie e a liderança dos bandos [...] . Durante as observações (em média de 2h30) foi quantificado o tempo vigiado por sentinelas de ambas as espécies. Os sentinelas de Cypsnagra hirundinacea realizaram a quase totalidade da vigilância (tempo com sentinela foi em média de 42 ± 17% do tempo, sendo 41 ± 17% vigiado por C. hirundinacea e 1,2% por N. fasciata), além de emitirem todos os chamados de alarme registrados (54% dos encontros com predadores aéreos estimularam chamados de alarme). O tempo vigiado por sentinelas nos bandos apresentou relação significativa com a taxa de encontro entre os predadores aéreos e os bandos (ANOVA, F = 3,0; p Abstract in english Bird mixed flocks including Cypsnagra hirundinacea and Neothraupis fasciata as species with sentinels were studied in "campo-cerrado" in order to investigate the possible relationship between alertness and the mixed flock leadership. This study was conducted from March to September 1996 and mixed fl [...] ocks were observed on average for 2:30h. The time with sentinels were recorded for C. hirundinacea and N. fasciata. The sentinels of Cypsnagra hirundinacea performed most of the vigilance (time with sentinel was on average 42 ± 17%, 41 ± 17% of which by C. hirundinacea, whereas only 1.2 % by N. fasciata) and gave all the alarm calls recorded (54% of the encounters with raptors stimulated alarm calls). A relationship was verified between time with sentinel and the rate of encounters with raptor (ANOVA, F = 3.0, P

J., RAGUSA-NETTO.

363

AcEST: DK952094 [AcEST  

Full Text Available TST38A01NGRL0013_C17 684 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0013_C17. 5' end seq ... a... 73 1e-12 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 72 3e-12 sp|P07465|PLAS_ ... OS=Nicotiana tabacum P... 71 5e-12 sp|P13133|PLAS_ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 71 ...

364

AcEST: DK952653 [AcEST  

Full Text Available TST38A01NGRL0014_K21 712 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0014_K21. 5' end seq ... a... 73 1e-12 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 72 3e-12 sp|P07465|PLAS_ ... OS=Nicotiana tabacum P... 71 5e-12 sp|P13133|PLAS_ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 71 ...

365

AcEST: DK951595 [AcEST  

Full Text Available TST38A01NGRL0011_N06 674 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0011_N06. 5' end seq ... a... 73 1e-12 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 72 3e-12 sp|P07465|PLAS_ ... OS=Nicotiana tabacum P... 71 5e-12 sp|P13133|PLAS_ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 71 ...

366

AcEST: BP917488 [AcEST  

Full Text Available YMU001_000101_D10 357 Adiantum capillus-veneris mRNA. clone: YMU001_000101_D10. BP917488 CL27Con ... o... 36 0.069 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 34 0.20 sp|P07465|PLAS_E ... eromorpha prolifera GN=P... 33 0.34 sp|P13133|PLAS_ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 33 ...

367

AcEST: DK945641 [AcEST  

Full Text Available YMU02A01NGRL0009_P19 579 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0009_P19. 5' end seq ... 1... 71 4e-12 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 70 6e-12 sp|P07465|PLAS_ ... cia faba GN=PETE PE=1 SV=1 69 1e-11 sp|P13133|PLAS_ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 69 ...

368

AcEST: DK952686 [AcEST  

Full Text Available TST38A01NGRL0014_M09 728 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0014_M09. 5' end seq ... a... 73 1e-12 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 72 3e-12 sp|P07465|PLAS_ ... OS=Nicotiana tabacum P... 71 5e-12 sp|P13133|PLAS_ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 71 ...

369

AcEST: DK943864 [AcEST  

Full Text Available YMU02A01NGRL0004_D05 602 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0004_D05. 5' end seq ... ... 49 3e-05 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 47 1e-04 sp|P13133|PLAS_ ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 45 2e-04 sp|P07465|PLAS_ ...

370

AcEST: DK952613 [AcEST  

Full Text Available TST38A01NGRL0014_J05 639 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0014_J05. 5' end seq ... a... 73 1e-12 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 72 2e-12 sp|P07465|PLAS_ ... OS=Nicotiana tabacum P... 71 4e-12 sp|P13133|PLAS_ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 71 ...

371

AcEST: DK952097 [AcEST  

Full Text Available TST38A01NGRL0013_C20 621 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0013_C20. 5' end seq ... a... 73 1e-12 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 72 2e-12 sp|P07465|PLAS_ ... OS=Nicotiana tabacum P... 71 4e-12 sp|P13133|PLAS_ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 71 ...

372

AcEST: BP919923 [AcEST  

Full Text Available YMU001_000130_G02 415 Adiantum capillus-veneris mRNA. clone: YMU001_000130_G02. BP919923 CL27Con ... o... 44 3e-04 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 42 7e-04 sp|Q9SXW9|PLAS_ ... isoform, chloroplastic ... 42 7e-04 sp|P13133|PLAS_ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 41 ...

373

AcEST: BP920088 [AcEST  

Full Text Available YMU001_000132_H03 428 Adiantum capillus-veneris mRNA. clone: YMU001_000132_H03. BP920088 CL27Con ... ... 42 9e-09 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 47 2e-08 sp|P00298|PLAS_ ... plastic OS=Silene prate... 42 4e-08 sp|P13133|PLAS_ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 45 ...

374

AcEST: DK947252 [AcEST  

Full Text Available YMU02A01NGRL0015_E22 473 Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0015_E22. 5' end seq ... teris crassirhizoma GN... 108 2e-25 sp|P13133|PLAS_ULVA R Plastocyanin OS=Ulva ... arasakii GN=PETE PE=1 ... 42 ... ... 42 0.002 sp|P56274|PLAS_ULVPE Plastocyanin OS=Ulva ... pertusa GN=PETE PE=1 SV=1 41 0.002 sp|P00298|PLAS_ ...

375

Utilização de macroalgas verdes como biorremediadores em efluentes de indústrias agroalimentares: dinâmica das vias metabólicas relacionadas com os compostos azotados  

Digital Repository Infrastructure Vision for European Research (DRIVER)

Ulva e Enteromorpha são duas das algas associadas a fenómenos de eutrofização, que ocorrem naturalmente na Ria Formosa, Algarve. Estas macroalgas verdes, normalmente consideradas como problema ambiental, podem ser vistas como uma solução de biorremediação para os problemas causados pelas descargas de resíduos e efluentes provenientes de agroindústrias. Este trabalho teve como objetivo principal o estudo da capacidade biorremediadora das algas Ulva e Enteromorpha. A atividade da nitr...

Madeira, He?lder Trindade

2013-01-01

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