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Purification and characterization of four keratinases produced by Streptomyces sp. strain 16 in native human foot skin medium  

British Library Electronic Table of Contents (United Kingdom)

Four extracellular keratinases (designated KI, KII, KIII, and KIV) were produced during submerged aerobic cultivation in a medium containing native human foot skin (NHFS) for enzyme synthesis. The molecular weights, determined by SDS-PAGE, were 25, 50, 34, and 19kDa, respectively. Gel filtration of the four purified enzymes in native conditions indicated that active keratinase KI is a novel homo-octamer, KII a homo-dimer, and KIII and KIV monomers. All four keratinases exhibited high activities at pH 8.0-10.0 with an optimal pH of 9.0. The optimal temperature for keratinolytic activity of KI, KII, and KIII was approximately 50, and 60degreeC for KIV. One millimolar of PMSF completely inhibited the keratinolytic activities of the four enzymes. The N-terminal sequences of KI, KII, and KIII s...

2010-01-01

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Elise plans and progress  

International Nuclear Information System (INIS)

Elise is a heavy ion induction linear accelerator that will demonstrate beam manipulations required in a driver for inertial fusion energy. With a line charge density similar to that of heavy ion drivers, Elise will accelerate a #>=# 1 gs beam pulse of K"+ ions from an initial energy of 2 MeV to a final energy #>=# 5 MeV. In the present design, the Elise electrostatic quadrupoles (ESQ) will have a 2.33 cm radius aperture operating at #+-#59 kV. The half-lattice periods range from 21 cm to 31 cm. The entire machine will be approximately 30 m long, half of that is the induction accelerator and the remaining half is the injector (including the Marx generator) and the matching section. Elise will be built in a way that allows future expansion into the full ILSE configuration, therefore it will have an array of four ESQ focusing channels capable of transporting up to a total of 3.2 A of beam current. Elise will also have an active alignment system with an ...

1995-09-06