u1-independent pre-mrna splicing: Topics by WorldWideScience.org

A novel Na⁺ channel splice form contributes to the regulation of an androgen-dependent social signal

BackgroundSubtle alternative splicing events involving tandem splice sites separated by a short (2-12 nucleotides) distance are frequent and evolutionarily widespread in eukaryotes,...Full Text Available

Sequence features involved in the mechanism of 3' splice junction wobbling

Na⁺ channels are often spliced but little is known about the functional consequences of splicing. We have been studying the regulation of Na⁺ current inactivation in an...Full Text Available

2008-09-10

Computational analysis of candidate intron regulatory elements for tissue-specific alternative pre-mRNA splicing

BackgroundAlternative splicing is an important mechanism mediating the diversified functions of genes in multicellular organisms, and such event occurs in around 40-60% of human...Full Text Available

Synaptic Signaling and Aberrant RNA Splicing in Autism Spectrum Disorders

No abstract prepared.

2001-04-01

In vitro dimerization of human immunodeficiency virus type 1 (HIV-1) spliced RNAs

Interactions between presynaptic and postsynaptic cellular adhesion molecules (CAMs) drive synapse maturation during development. These trans-synaptic interactions are regulated by alternative splicing...Full Text Available

Defective hepatitis B virus particles are generated by packaging and reverse transcription of spliced viral RNAs in vivo.

The human immunodeficiency virus type 1 (HIV-1) packages its genomic RNA as a dimer of homologous RNA molecules that has to be selected among a multitude of cellular and viral RNAs. Interestingly, spliced...Full Text Available

2007-12-01

Conserved Alternative Splicing and Expression Patterns of Arthropod N-Cadherin

Generation of replicative defective viruses is frequently observed during viral infections. We now report that encapsidation and reverse transcription of spliced viral RNA is an additional mechanism...Full Text Available

1991-10-01

Differential regulation of iPLA2beta splice variants by in vitro ischemia in C2C12 myotubes

Metazoan development requires complex mechanisms to generate cells with diverse function. Alternative splicing of pre-mRNA not only expands proteomic diversity but also provides a means to regulate...Full Text Available

2009-04-01

DEFF Research Database (Denmark)

Udgivelsesdato: March

2006-01-01

DESIGN, MANUFACTURE, DEVELOPMENT, TEST, AND EVALUATION OF BORON ...

Science.gov (United States)

the shear-beam design and the larger scale component test specimen. The subcompo - nents designed were: a. Web splice specimen. ...

Mechanism of Dephosphorylation of the SR Protein ASF/SF2 by Protein Phosphatase 1

British Library Electronic Table of Contents (United Kingdom)

SR proteins are essential splicing factors whose function is controlled by multi-site phosphorylation of a C-terminal domain rich in arginine-serine repeats (RS domain). The protein kinase SRPK1 has been shown to polyphosphorylate the N-terminal portion of the RS domain (RS1) of the SR protein ASF/SF2, a modification that promotes nuclear entry of this splicing factor and engagement in splicing function. Later, dephosphorylation is required for maturation of the spliceosome and other RNA processing steps. While phosphates are attached to RS1 in a sequential manner by SRPK1, little is known about how they are removed. To investigate factors that control dephosphorylation, we monitored region-specific mapping of phosphorylation sites in ASF/SF2 as a function of the protein phosphatase PP1. W...

2010-01-01

The Vasoactive Potential of Kisspeptin-10 in the Peripheral Vasculature

Splice assembly tool and method of splicing

Splice products of the Kiss1 protein (kisspeptins) have been shown to be involved in a diverse range of functions, including puberty, metastasis and vasoconstriction in large human arteries. Circulating...Full Text Available

Improving the Arabidopsis genome annotation using maximal transcript alignment assemblies

A splice assembly tool for assembling component parts of an electrical conductor while producing a splice connection between electrical cables therewith, comprises a first structural member adaptable for supporting force applying means thereon, said force applying means enabling a rotary force applied manually thereto to be converted to a longitudinal force for subsequent application against a first component part of said electrical connection, a second structural member adaptable for engaging a second component part in a manner to assist said first structural member in assembling the component parts relative to one another and transmission means for conveying said longitudinal force between said first and said second structural members, said first and said second structural members being coupled to one another by said transmission means, wherein at least one of said component parts comprises a tubular elastomeric sleeve and said force applying ...

1980-01-01

Characterization, phylogeny, alternative splicing and expression of Sox30 gene

The spliced alignment of expressed sequence data to genomic sequence has proven a key tool in the comprehensive annotation of genes in eukaryotic genomes. A novel algorithm was developed to assemble...Full Text Available

2003-10-01

A new VCAN/versican splice acceptor site mutation in a French Wagner family associated with vascular and inflammatory ocular features

BackgroundMembers of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including...Full Text Available

The human gastrin/cholecystokinin type B receptor gene: alternative splice donor site in exon 4 generates two variant mRNAs.

PurposeTo detail the highly variable ocular phenotypes of a French family affected with an autosomal dominantly inherited vitreoretinopathy and to identify the disease gene.MethodsSixteen...Full Text Available

The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding.

Gastrin and its carboxyl-terminal homolog cholecystokinin (CCK) exert a variety of biological actions in the brain and gastrointestinal tract that are mediated in part through one or more G protein-coupled...Full Text Available

1993-10-01

Selective Expression in Carotid Body Type I Cells of a Single Splice Variant of the Large Conductance Calcium- and Voltage-activated Potassium Channel Confers Regulation by AMP-activated Protein Kinase*

We have isolated and sequenced cDNA clones encoding the human U1-70K snRNP protein, and have mapped this locus (U1AP1) to human chromosome 19. The gene produces two size classes of RNA, a major 1.7-kb...Full Text Available

1987-12-23

Identification of a Drosophila G protein alpha subunit (dGq alpha-3) expressed in chemosensory cells and central neurons.

Inhibition of large conductance calcium-activated potassium (BK_Ca) channels mediates, in part, oxygen sensing by carotid body type I cells. However, BK_Ca channels remain active...Full Text Available

2011-04-08

1 2 3

Expression of alternatively spliced human T-lymphotropic virus type I pX mRNA in infected cell lines and in primary uncultured cells from patients with adult T-cell leukemia/lymphoma and healthy carriers.

We have identified another Drosophila GTP-binding protein (G protein) alpha subunit, dGq alpha-3. Transcripts encoding dGq alpha-3 are derived from alternative splicing of the dGq alpha locus previously...Full Text Available

1995-12-05

Evaluation of 230 kV HPFF pipe-type cable with wrinkled and creased insulating tapes

Although human T-cell lymphotropic virus type I (HTLV-I) is the etiologic agent of adult T-cell leukemia/lymphoma (ATL), the role of viral gene expression in the progression to and maintenance of the...Full Text Available

1992-04-01

Analysis of the transcript encoding the latent Epstein-Barr virus nuclear antigen I: a potentially polycistronic message generated by long-range splicing of several exons.

Severe collapse wrinkles and circumferential creases were discovered in the cellulose paper insulating tapes of a newly installed IIPFF pipe-type cable during splicing and terminating. An evaluation program was developed to assess the electrical and mechanical integrity of the cable having wrinkled and creased insulating tapes. The test results indicated that the cable would perform satisfactorily in service.

1995-01-01

Alternative mRNA Splicing Produces a Novel Biologically Active Short Isoform of PGC-1?*

The Epstein-Barr virus nuclear antigen (EBNA I) present in latently infected cells is encoded in a 2-kilobase exon contained in the BamHI K viral genomic fragment. This exon is, however, found within...Full Text Available

1985-12-01

A Targeted Bypass Screen Identifies Ynl187p, Prp42p, Snu71p, and Cbp80p for Stable U1 snRNP/Pre-mRNA Interaction?

The transcriptional co-activator PGC-1α regulates functional plasticity in adipose tissue by linking sympathetic input to the transcriptional program of adaptive thermogenesis. We report here...Full Text Available

2009-11-20

PILC cable technology and accessories

To understand how DEXD/H-box proteins recognize and interact with their cellular substrates, we have been studying Prp28p, a DEXD/H-box splicing factor required for switching the U1 snRNP with the U6...Full Text Available

2009-07-01

Detection of Second-Layer Corrosion in Aging Aircraft Fuselage

Studies were conducted to examine the pressure on fluid-filled cables of electric power transmission networks in the event of an oil leak. Transition splices between paper-insulated oil-impregnated cable systems (PILC) and solid dielectric cable is difficult to perform. A proper PILC is composed of a means to contain oil within the cable structure and to keep moisture out. A failure of the cable joint could occur if an oil leak dries out the paper. Since oil pressure in most PILC cables is high, strong mechanical means must be devised to contain the oil within the cable. Resin systems, heat shrink systems and wiped lead sleeves to reseal the PILC cable over the splice, are among the methods used to stop an oil leak. A system has been designed using an EPDM tube contained under mechanical pressure; it has proven to be effective over a wide range of pressure conditions. Test results utilizing this system were presented. 1 ref., 1 tab., 4 figs.

1996-08-01

[Molecular analysis of cytoplasmic male sterility]. Progress report, June 1, 1989--June 15, 1991

A Digital X-ray imaging system using Compton backscattering has been developed to obtain a cross-sectional profile and mass loss of corroded lap-splices of aging aircraft from density variation. A slit-type camera was designed to focus on a small scattering volume inside the material, from which the backscattered photons are collected by a collimated scintillator detector for interpretation of material characteristics. The cross section of the lap-joint is scanned by moving the scattering volume through the thickness direction of the specimen. The mass loss of each layer has been estimated from a Compton backscatter A-scan to obtain the thickness of each layer including the aluminum sheet, the corrosion layer and the sealant. Quantitative information such as location and width of planar corrosion in the lap splices of fuselages is obtained by deconvolution using a nonlinear least-square error minimization method(BFGS method): A simple ...

2006-12-01

Tissue-specific expression of Fgfr2b and Fgfr2c isoforms, Fgf10 and Fgf9 in the developing chick mandible

We have determined the actual RNA sequence of four mitochondrial genes: pcf, nad3, rps12, and coxII. Because plant mitochondrial proteins cannot be predicted from DNA sequences, the actual primary structures of the encoded proteins were unknown. We have gained information concerning the process of RNA editing. Editing can occur before splicing. Our data is consistent with the hypothesis that RNA editing is not simultaneous with transcription. Unlike other systems, the process of editing in plant does not exhibit a discernable direction. As a result of our RNA editing studies, we have produced a number of clones of the proper coding regions tube used for incorporating these mitochondrial genes into the nucleus. 6 refs., 4 figs.

1991-12-31

British Library Electronic Table of Contents (United Kingdom)

SummaryExperimental evidence has demonstrated the importance of FGF signalling in morphogenesis of the mandibular processes. FGFs transmit their signals through four tyrosine kinase transmembrane receptors (FGFRs). Alternative splicing in FGFRs including FGFR2 generates different isoforms that exhibit different ligand-specificities, exclusive tissue distributions and specific biological functions. Despite extensive information regarding the isoform-specific patterns of expression Fgfr2c and Fgfr2b during morphogenesis of many organs, a comparative analysis of these specific isoforms in the chick mandible has not been reported. To better understand the function of FGFR2 in mandibular morphogenesis, we have analysed the expression Fgfr2b, Fgfr2c and their putative ligands Fgf10 and Fgf9, in ...

2006-01-01

Selective changes of retroelement expression in human prostate cancer

British Library Electronic Table of Contents (United Kingdom)

Retroelements constitute a large part of the human genome. These sequences are mostly silenced in normal cells, but genome-wide DNA hypomethylation in cancers might lead to their re-expression. Whether this re-expression really occurs in human cancers is largely unkown. We therefore investigated expression and DNA methylation of several classes of retroelements in human prostate cancer tissues and cell lines by quantitative reverse transcription-polymerase chain reaction and pyrosequencing, respectively. The most striking finding was strong and generalized increased expression of the HERV-K_22q11.23 provirus in cancers, including de novo expression of a spliced accessory Np9 transcript in some tumors. In parallel, DNA methylation in the long terminal repeat (LTR) decreased. Conversely, HER...

2011-01-01

Levels of mesenchymal FGFR2 signaling modulate smooth muscle progenitor cell commitment in the lung

British Library Electronic Table of Contents (United Kingdom)

Fibroblast growth factor (FGF) signaling has been shown to regulate lung epithelial development but its influence on mesenchymal differentiation has been poorly investigated. To study the role of mesenchymal FGF signaling in the differentiation of the mesenchyme and its impact on epithelial morphogenesis, we took advantage of Fgfr2c+/? mice, which due to a splicing switch express Fgfr2b in mesenchymal tissues and manifest Apert syndrome-like phenotypes. Using a set of in vivo and in vitro studies, we show that an autocrine FGF10?FGFR2b signaling loop is established in the mutant lung mesenchyme, which has several consequences. It prevents the entry of the smooth muscle progenitors into the smooth muscle cell (SMC) lineage and results in reduced fibronectin and elastin deposition. Levels of...

2006-01-01

Identification of mutations leading to the Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA

GFAP in health and disease

The Lesch-Nyhan (LN) syndrome is a severe X chromosome-linked disease that results from a deficiency of the purine salvage enzyme hypoxanthine phosphoribosyltransferase (HPRT). The mutations leading to the disease are heterogeneous and frequently arise as de novo events. The authors have identified nucleotide alterations in 15 independently arising HPRT-deficiency cases by direct DNA sequencing of in vitro amplified HPRT cDNA. They also demonstrate that the direct DNA sequence analysis can be automated, further simplifying the detection of new mutations at this locus. The mutations include DNA base substitutions, small DNA deletions, a single DNA base insertion, and errors in RNA splicing. The application of these procedures allows DNA diagnosis and carrier identification by the direct detection of the mutant alleles within individual families affected by LN.

1989-03-01

British Library Electronic Table of Contents (United Kingdom)

Glial fibrillary acidic protein (GFAP) is the main intermediate filament protein in mature astrocytes, but also an important component of the cytoskeleton in astrocytes during development. Major recent developments in astrocyte biology and the discovery of novel intermediate filament functions enticed the interest in the function of GFAP. The discovery of various GFAP splice variants gave an additional boost to explore this protein in more detail.The structural role of GFAP in astrocytes has been widely accepted for a long time, but over the years, GFAP has been shown to be involved in astrocyte functions, which are important during regeneration, synaptic plasticity and reactive gliosis. Moreover, different subpopulations of astrocytes have been identified, which are likely to have distinc...

2011-01-01

Cloning of the cDNA and gene for a human D sub 2 dopamine receptor

Repair of failed 138kV pipe type cable line

A clone encoding a human D{sub 2} dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D{sub 2} receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.

1989-12-01

Non-Destructive Testing of Bus-Bar Joints Powering LHC Superconducting Magnets, by Using Gamma Sources

On February 9, 1995 ComEd experienced a pipe type cable failure on 138kV line 13717. Line 1737 is a bulk power transmission cable circuit connecting The Fisk and Crawford generating stations located within city limits. The line is approximately 4.5 miles long and was originally installed in 1975. This line is forced cooled, has 2,500 KCMIL copper conductors with 505 mils of paper insulation in an 8 inch pritec coated steel pipe. The splices are constructed with Voltalit castings. The failure occurred approximately 3,500 feet from Crawford Station. It was easy to locate the failure since the cable joint casing was breached resulting in the loss of approximately 2,600--2,700 gallons of dielectric fluid. Upon field examination B Phase was determined to have failed. The failure occurred 12 inches inside the casing in an area where it was impossible to install a long connector or add an additional short length of cable to create a piece out. The cause of the failure is ...

1996-11-01

CERN Document Server

The main LHC superconducting magnets (dipoles and quadrupoles) use Rutherford type cables, stabilized electrically and thermally with copper profiles. The portions of cables are connected to each other by a soft soldering technique (Sn96Ag4) with an overlapping length corresponding to one pitch of the superconducting strands. The splice constitutes a "composite" structure with the interchanging layers of Sn96Ag4 and NbTi superconductor, located inside a Cu cage. In order to ensure a high level of reliability (failure probability not exceeding 10-8) for some 10000 connections in the LHC, a non-destructive technique to check the quantity of solder in the joint is foreseen. The technique is based on a gamma ray source (241Am) and the detection is position-sensitive in the transmission mode. Scintillating detectors of gamma rays are used and their accumulated length corresponds to the length of the radioactive source (120 mm). The method can be used in-situ, the ...

2004-01-01

Laboratory development of +- 600 kVdc pipe type cable systems

Heat shock factor binds to heat shock elements upstream of heat shock protein 70a and Samui genes to confer transcriptional activity in Bombyx mori diapause eggs exposed to 5^oC

In 1976, the US Department of Energy and New York State - ERDA granted a contract for the development of a +-600 kV dc underground transmission cable system looking to inevitable future requirements in the US for economic, high-capacity, long-distance underground transmission linking remote generating stations to metropolitan load centers. This project was comprehensive and dealt with three separate system components, i.e., cable, splice, and terminals. In each instance, for each component, development required an intensive R and D effort, focused on the following categories: insulating materials, model testing, design of full-scale prototype, manufacturer of prototype, and laboratory development testing. Insulating papers and oils were selected, and model studies performed, for both self-contained type and pipe type oil-filled cables. However, only the pipe cable design was finally designated for full-size cable manufacture and EHV laboratory testing. Each of the ...

1982-07-01

British Library Electronic Table of Contents (United Kingdom)

To understand the molecular mechanisms of how 5^oC-incubation activates mRNA expression of Hsp70a and Samui genes in Bombyx mori diapause eggs, we first searched the 5'-upstream regions of the Hsp70a and Samui genes for heat shock elements (HSEs) and found two regions [Hsp70aHSE-1 (-95 to -58) and -2 (-145 to -121), and SamuiHSE-1 (-84 to -55) and -2 (-304 to -290)] corresponding to HSEs (repeats of nGAAn and/or nTTCn). We cloned four cDNAs encoding heat shock factor (HSF)-a2 (627 amino acids), -b (685 aa), -c (682 aa) and -d (705 aa), which were produced by alternative splicing. When we exposed diapause eggs to 5^oC beginning at 2 day post-oviposition to break diapause, HSFd mRNA only increased after chilling for 6-8 days, a pattern very similar to those of Hsp70a and Samui mRNAs. To exam...

2011-01-01

Free and forced convective cooling of pipe-type electric cables. Volume 2: electrohycrodynamic pumping. Final report

Free and forced convective cooling of pipe-type electric cables. Volume 1: forced cooling of cables. Final report

A multi-faceted research program has been performed to investigate in detail several aspects of free and forced convective cooling of underground electric cable systems. There were two main areas of investigation. The first one, reported in Volume 1, dealt with the fluid dynamic and thermal aspects of various components of the cable system. In particular, friction factors for laminar flow in the cable pipes with various configurations were determined using a finite element technique; the temperature distributions and heat transfer in splices were examined using a combined analytical numerical technique; the pressure drop and heat transfer characteristics of cable pipes in the transitional and turbulent flow regime were determined experimentally in a model study; and full-scale model experimental work was carried out to determine the fluid dynamic and thermal characteristics of entrance and exit chambers for the cooling oil. The second major area of activity, ...

1981-05-01

1 2 3

Dynamic Rating and Underground Monitoring System (DRUMS)

A multi-faceted research program has been performed to investigate in detail several aspects of free and forced convective cooling of underground electric cable systems. There were two main areas of investigation. The first one reported in this volume dealt with the fluid dynamic and thermal aspects of various components of the cable system. In particular, friction factors for laminar flow in the cable pipes with various configurations were determined using a finite element technique; the temperature distributions and heat transfer in splices were examined using a combined analytical numerical technique; the pressure drop and heat transfer characteristics of cable pipes in the transitional and turbulent flow regime were determined experimentally in a model study; and full-scale model experimental work was carried out to determine the fluid dynamic and thermal characteristics of entrance and exit chambers for the cooling oil. The second major area of activity, ...

1981-05-01

Detection of Second-Layer Corrosion in Aging Aircraft

Drums is an acronym for Dynamic Rating and Underground Monitoring System, with specific application to high-pressure pipe-type cable systems which are the predominant means of underground electric power transmission in the United States. The primary objective of this EPRI-sponsored feasibility study was to establish the technical feasibility of seven DRUMS functions and two novel DRUMS communication schemes. The seven DRUMS functions which monitor the system's vital and measurable parameters in real time and perform system diagnostics in the event of component deterioration, malfunction or failure are: Real Time Current Monitoring and Rating; Leak Detection and Location; Fault Location; Pothead Monitoring; Oil Monitoring; Pipe Protection; and Splice Monitoring. The two DRUMS communication schemes are internal to the pipe cable system. Mechanical Communication will use pressure pulses to transmit data along the system, while the Electrical Communication ...

1991-07-01

Use of polymer conductors for welding thermoplastic materials

The Compton backscatter technique has been applied to lap-joint in aircraft structure in order to determine mass loss due to exfoliative corrosion of the aluminum alloy sheet skin. The mass loss of each layer has been estimated from Compton backscatter A-scan including the aluminum sheet, the corrosion layer, and the sealant. A Compton backscattering imaging system has been also developed to obtain a cross-sectional profile of corroded lap-splices of aging aircraft using a specially designed slit-type camera. The camera is to focus on a small scattering volume inside the material from which the backscattered photons are collected by a collimated scintillator detector for interpretation of material characteristics. The cross section of the layered structure is scanned by moving the scattering volume through the thickness direction of the specimen. The theoretical model of the Compton scattering based on Boltzmann transport theory is presented for quantitative ...

2009-12-01

Molecular evolution and characterization of fungal indoleamine 2,3-dioxygenases.

Although some polymer conductors of electricity (ex. Polyanilines) are materials known for more than 100 years, only recently have the interesting chemical, electrical and optic properties of their insulating and conducting forms been recognized. Advances made in the chemistry of polymer conductors have also led to improvements in processing them. This work studies a practical application of these materials: the use of polymer conductors for the remote welding of insulating thermoplastic polymers, using energy from microwaves for the local heating of the union. Many thermoplastics (for ex. Polyethylene) do not absorb, or absorb very little, energy from microwaves. Different conductor materials (conductor polymers, carbon nanotubes), however, heavily absorb energy from microwaves with the resulting heating. In this way the welding zone can be heated without affecting the rest of the piece. Conductor polymers (polyanilines) were synthesized chemically and they were characterized by ...

2004-11-01

Science.gov (United States)

Indoleamine 2,3-dioxygenase (IDO) and tryptophan 2,3-dioxygenase (TDO) are tryptophan-degrading enzymes. Mammalian IDO expression is induced by cytokines and has antimicrobial and immunomodulatory effects. A major role of mammalian TDO is to supply nicotinamide adenine dinucleotide (NAD(+)). In fungi, the IDO homologue is thought to be expressed constitutively and supply NAD(+), as TDO is absent from their genomes. Here, we reveal the distribution of IDO genes among fungal species and characterize their enzymatic activity. The yeast, Saccharomyces cerevisiae has only one IDO gene, whereas the koji-mold, Aspergillus oryzae has two genes, IDO? and IDO?. The A. oryzae IDO? showed more similar enzymatic properties to those of S. cerevisiae IDO than IDO?, suggesting that the A. oryzae IDO? is a functional homologue of the S. cerevisiae IDO. From the IDO? gene, two isoforms, IDO? and IDO?(+) could be generated by alternative splicing. The latter contained a 17 amino ...

2010-12-18

Expression and function of fibroblast growth factor (FGF) 9 in hepatic stellate cells and its role in toxic liver injury

An ultraviolet-inducible adenosine-adenosine cross-link reflects the catalytic structure of the Tetrahymena ribozyme

Hepatic injury and regeneration of the liver are associated with activation of hepatic stellate cells (HSC). Fibroblast growth factors (FGFs) and their receptors are important regulators of repair in various tissues. HSC express FGFR3IIIc as well as FGFGR4 and different spliced FGFR1IIIc and FGFR2IIIc isoforms which differ in the presence or absence of the acid box and of the first Ig-like domain. Expression of FGF9, known to be capable to activate the HSC FGFR2/3-isoforms, was increased in HSC in liver slice cultures after exposition to carbon tetrachloride, as an acute liver injury model. FGF9 significantly stimulated 3-H thymidine incorporation of hepatocytes, but failed to induce DNA synthesis in HSC despite the fact that FGF9 induced a sustained activation of extracellular signal-related kinases (ERK) 1/2. FGF9 induced an increased phosphorylation of Tyr436 of the fibroblast growth factor receptor substrate (FRS) 2, while phosphorylation of Tyr196 which is ...

2007-09-21